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Sample records for cytectrene marque par

  1. New neutral and lipophilic technetium complexes based on a cytectrene moiety. Synthesis, characterization and biological evaluation

    Energy Technology Data Exchange (ETDEWEB)

    El Aissi, Radhia [National Centre of Sciences and Nuclear Technology, Sidi Thabet (Tunisia). Radiopharmaceutical Unit; CNRS, Laboratoire de Synthese et Physico-Chimie de Molecules d' Interet Biologique, SPCMIB, UMR 5068, Toulouse (France); Toulouse Univ., UPS, Laboratoire de Synthese et Physico-Chimie de Molecules d' Interet Biologique, SPCMIB, UMR 5068, Toulouse (France); Malek-Saied, Nadia; Saidi, Mouldi [National Centre of Sciences and Nuclear Technology, Sidi Thabet (Tunisia). Radiopharmaceutical Unit; Mallet-Ladeira, Sonia [Toulouse Univ., UPS et CNRS, FR2599 (France). Inst. de Chimie de Toulouse; Coulais, Yvon [Toulouse Univ. (France). Lab. ' ' Traceurs et traitement de l' image' ' ; Benoist, Eric [CNRS, Laboratoire de Synthese et Physico-Chimie de Molecules d' Interet Biologique, SPCMIB, UMR 5068, Toulouse (France); Toulouse Univ., UPS, Laboratoire de Synthese et Physico-Chimie de Molecules d' Interet Biologique, SPCMIB, UMR 5068, Toulouse (France)

    2015-05-01

    The synthesis, characterization and biological evaluation of five neutral and lipophilic {sup 99m}Tc-complexes, so-called cytectrenes, obtained from N-substitutedferrocenecarboxamide derivatives are reported. N-substituted ferrocenecarboxamide starting materials were obtained in two steps, with good yield and were fully characterized by classical spectroscopic methods including X-ray diffraction analysis for one of them. Using a microwave strategy for the {sup 99m}Tc-radiolabelling step, each cytectrene were obtained quickly (radiolabelling time < 5 min), from modest to good yield. The {sup 99m}Tc-complexes, characterized by HPLC comparison with cold rhenium complex analogues, are stable, neutral and lipophilic (logP{sub o/w} ranged between 1.8 and 2.9). Unfortunately, despite such suitable features, in vivo studies of two of them gave poor results, in terms of brain uptake. Both radiocompounds exhibited the maximum brain accumulation of 0.31% ID/g and 0.26% ID/g at 5 min post-injection, respectively, followed by a very fast washout from the brain (0.06% ID/g and 0.07% ID/g at 30 min post-injection, respectively). Although our ligand systems exhibited high stability against exchange reactions with blood proteins, the high radioactivity level in stomach, increasing with time, suggests in vivo decomposition of our complex to pertechnetate.

  2. Continuité du discours de la marque et efficacité de la communication

    OpenAIRE

    Darpy, Denis

    2010-01-01

    La vie d'une marque est rythmée par l'innovation, les changements organisationnels, les bouleversements technologiques... Les discontinuités peuvent être dangereuses dans la construction du capital de la marque. Toutefois, la rupture permanente pour une marque peut s'inscrire dans une continuité (cf. Virgin cassant les monopoles). Au travers d'exemples récents, on identifie plusieurs types de marques possibles (« provocante », « guérilla », « marque qui se cherche », «évolution harmonieuse »)...

  3. Marques de Melo: El inventor de tradiciones

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    Gustavo Cimadevilla

    2008-12-01

    Full Text Available Este texto es un acercamiento académico, emotivo y grato, del autor al maestro. Marques de Melo es un inventor de tradiciones simbólicas, icónicas que están en la realidad y que son las forjadoras de una cultura que se resiste a ser vencida.

  4. A vos marques! an accelerated French course

    CERN Document Server

    Andrews, Alison; Tippett-Spirtou, Sandy

    2012-01-01

    A Vos Marques! is an introductory course for students taking French as an option alongside their main degree course. It has been developed specifically for false beginners: students who have a slight acquaintance with the language.The course comprises a student's book, teacher's book and three 60-minute audio cassettes and, through fifteen chapters, follows the progress of an English-speaking student studying in Paris, whose achievements are designed to reflect those of the course user.Special features include:* activities involving pair and group work* an aid to self-

  5. Stratégies de marque : pour quelle création de valeur ? Application aux entreprises agroalimentaires du Languedoc-Roussillon

    OpenAIRE

    Béatrice Siadou-Martin; Philippe Aurier; Jean-Pierre Couderc

    2012-01-01

    La question posée dans la présente contribution est de savoir dans quelle mesure la « stratégie de marquage » retenue par les Petites et Moyennes Entreprises du secteur agroalimentaire influence leur création de valeur et leurs résultats financiers. L’utilisation d’une marque ou non, ainsi que les « modes de marquage » que sont la marque propre, la marque de distributeur ou d’un autre producteur seront indifféremment qualifiés de stratégies (ou politiques) de marquage ou de marque dans ce...

  6. Tiago Marques Aipobureu: um bororo marginal Tiago Marques Aipobureu: a Bororo on the margins

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    Florestan Fernandes

    2007-11-01

    Full Text Available O texto analisa a biografia de um membro da tribo dos Bororo, Tiago Marques Aipobureu, que, nascido no planalto oriental de Mato Grosso, foi levado pelos missionários para estudar em Cuiabá e depois viajou pela Europa. Quando de seu retorno ao Brasil, foi empregado como professor, mas não conseguiu adaptar-se à nova vida. Casado com uma mulher de sua tribo, buscou então reintegrar-se aos Bororo, igualmente sem sucesso. Permanecia, desse modo, no meio do caminho, incapaz de integrar-se plenamente tanto à sociedade e cultura "civilizadas" como à sociedade e cultura indígenas. O desajustamento de Tiago evidencia a situação do homem marginal, daquele que se situa na divisa de duas raças, na margem de duas culturas sem conseguir pertencer integralmente a nenhuma delas.The text analyzes the biography of a member of the Bororo tribe, Tiago Marques Aipobureu. Born on the eastern plateau of Mato Grosso, he was taken by missionaries to study in Cuiabá and later travelled through Europe. On returning to Brazil, he was employed as a teacher, but was unable to adapt to his new life. Married to a woman from his tribe, he tried to rejoin the Bororo, likewise without success. Thus he found himself midway between worlds, unable to join fully either 'civilized' society and culture, or indigenous society and culture. Tiago's maladjustment exemplifies the situation of the marginal person, someone situated on the dividing line between two races, on the margin of two cultures, unable to belong wholly to either.

  7. DOE Theory Graduate Student Fellowship: Gustavo Marques Tavares

    Energy Technology Data Exchange (ETDEWEB)

    Schmaltz, Martin [Boston Univ., MA (United States). Physics Dept.

    2015-12-30

    Marques Tavares was awarded a fellowship for his proposal “The ttbar asymmetry and beyond” to starting in September 2012. This is the final report summarizing the research activities and accomplishments achieved with this grant support. With support from the DOE graduate fellowship Marques Tavares, Katz and Xu at BU have investigated a new technique for obtaining quantitative results in strongly coupled field theories with broken conformal invariance. Such theories are especially interesting as they may be candidates for physics beyond the standard model with possible applications to strongly coupled electroweak symmetry breaking. However, because of the strong coupling even qualitative results about the spectrum of such theories are not rigorously understood.

  8. Marques de Melo: Su pensamiento periodístico

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    Jorge Pedro Sousa

    2008-12-01

    Full Text Available Marques de Melo el periodista investigador, cuyo trabajo es relevante para todo el universo de las Ciencias de la Comunicación, fue el primero en reconocer la existencia de una escuela de pensamiento comunicacional latinoamericano. El texto hace una breve incursión por los caminos seguidos por el teórico del periodismo.

  9. Evidence of a genetic instability induced by the incorporation of a DNA precursor marked with tritium; Mise en evidence d'une instabilite genetique induite par l'incorporation d'un precurseur de L'ADN marque au tritium

    Energy Technology Data Exchange (ETDEWEB)

    Saintigny, Y.; Laurent, D.; Lahayel, J.B. [CEA Fontenay-aux-Roses, IRCM-LRTS, U967 - CEA/INSERM/Universites Paris 7 and Paris-11, 92 (France); Roche, St.; Meynard, D.; Lopez, B.S. [CEA Fontenay-aux-Roses, LMR - UMR 217 - CEA/CNRS, Institut de Radiobiologie Cellulaire et moleculaire, Direction des Sciences du Vivant, 92 (France)

    2009-07-01

    The authors report a molecular geno-toxicology investigation which allowed molecular events induced par intracellular incorporation of tritium to be studied, and the genetic instability resulting from a chronic exposure even at low dose to be analysed. For this purpose, they developed cell models (hamster tumorous cells and human fibroblasts) in which they know how to incorporate given quantities of marked nucleotides in the DNA. They show that the incorporation of tritium, even with doses which are said to be non toxic, causes a prolonged exposure of the cell to a genotoxic stress, and maybe a genetic instability due to a too great number of recombination events

  10. Trois types de stratégies des fabricants pour la fidélisation aux médicaments de marque

    OpenAIRE

    Prémont, Marie-Claude; Gagnon, Marc-André

    2014-01-01

    La restructuration de l'industrie pharmaceutique a mené au développement de trois nouveaux types de stratégies commerciales pour la fidélisation de différentes cohortes de patients à des médicaments: la fidélisation par le rabais, par l'accompagnement et par la compassion. La fidélisation par le rabais vise à maintenir les traitements au produit de marque et décourager la substitution au produit générique. La fidélisation par l'accompagnement est basée sur une offre des services de suivi et d...

  11. El perfil arquetípic de les marques

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    Sabrine Dornelles

    2013-02-01

    Full Text Available Aquest article presenta una manera fàcil d'establir què significa una marca per als públics. Per això, es basa en l'inconscient col · lectiu fonamentat en les creences que tenim tots els éssers humans sobre els déus de la mitologia grega. El mètode fonamentat en l'estudi qualitatiu que permet definir els atributs de l'inconscient col · lectiu, utilitza la metodologia experimental per a concretar en base a un test de recepció el significat de les marques. L'estudi mostra els detalls de la significació i la riquesa de les marques en aquest sentit. Els resultats de l'estudi ens indica que les tres marques estudiades representen elements dels següents arquetips: Nike el d'adolescent, celebritat, explorador, heroi i inventor; Adidas el d'esposa, inventor i artista; i Puma el d'adolescent i príncep.

  12. A SUBVERSÃO DE NÚBIA NASCIMENTO MARQUES ATRAVÉS DA POESIA E DO ROMANCE

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    Elaine Almeida Aires Melnikoff

    2017-11-01

    Ce texte vise à analyser la trajectoire artistique de Núbia Marques, en soulignant la poète et le romancier. Dans sa longue carrière, cet artiste a joué dans plusieurs poèmes et romans primés. Ainsi, nous allons analyser le genre de l'écriture que l'écrivain réfléchit à écrire. Pour donner un caractère durable au texte, nous aborderons les concepts de Literary Field of Bourdieu (1996, Représentation de Chartier (1997. La méthodologie adoptée était la recherche historique, de la trajectoire de l'écrivain Nubia Marques, soutenu par l'hypothèse de l'histoire culturelle. Par conséquent, tout au long de la recherche il a été possible de percevoir que l'auteur a écrit ce qu'elle ressentait, et elle a souvent parlé d'elle-même et a placé des pseudonymes sur les personnages. Mots-clés: Poète; Écrivain Sergipana; Femme Intellectuelle.

  13. SCS118 Marques – New rice cultivar obtained through induced mutation

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    Moacir Antonio Schiocchet

    2014-03-01

    Full Text Available The new rice cultivar, SCS118 Marques, was obtained through gamma irradiation of SCSBRS Tio Taka cultivar. SCS118 Marques presents modern architecture, lodging resistance, late maturity cycle, moderate resistance to blast, high yield potential, long grains and very high cooking quality. Industrial tests performed with SCS118 Marques showed that grains are suitable for parboiling and white rice, and it is recommended to all rice-producing regions of Santa Catarina

  14. Biting behavior of Anopheles mosquitoes in Costa Marques, Rondonia, Brazil

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    Terry A. Klein

    1991-03-01

    Full Text Available Mosquito collections were made in and near Costa Marques, Rondonia, Brazil, to determine anopheline anthropophilic/zoophilic behavior. Collections from a non-illuminated, bovine-baited trap and indoor and outdoor human-bait collections were compared. Anopheles darlingi and Anopheles deaneorum were more anthropophilic than the other anophelines collected. The remainder of the Anopheles species were collected much morefrequently in bovine-baited traps than in human-bait collections. Anopheles darlingi and An. deaneorum were more frequently collected inside houses than the other anopheline species. But, when collections were made in a house with numerous openings in the walls, there were few differences in the percentages of each species biting man indoors versus outdoors. Anopheles darlingi was the predominant mosquito collected, both inside and outside houses, and had the strongest anthropophilic feeding behavior of the anophelines present.Para determinar o comportamento antropofilico e zoofilico dos anofelinos, foram capturados mosquitos na periferia e na zona urbana de Costa Marques, Rondônia, Brasil. Foram comparadas as capturas feitas à noite, com iscas bovinas e humanas, dentro efora de casa. O Anopheles darlingi e o Anopheles deaneorumforam mais antropojilicos do que os outros anofelinos capturados. O restante das espécies anofelinas foi capturado mais freqüentemente nas iscas bovinas do que nas humanas. Anopheles darlingi e Anopheles deaneorumforam capturados dentro de casa com mais freqüência do que as outras espécies anofelinas. Porém, quando a captura foi feita em casas com muitas aberturas nas paredes houve pouca diferença nas porcentagens de cada espécie sugadora de humanos dentro efora de casa. Anopheles darlingi foi o mosquito capturado com mais freqüência, dentro e fora de casa, e apresentava maior antropofilia em relação aos outros anofelinos presentes.

  15. Work and trickery in Marafa by Marques Rebelo

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    Manoel Freire

    2016-12-01

    In Marafa, Marques Rebelo concocts the social dynamics in Rio de Janeiro, bringing into light a great character gallery to compose two distinct universes, which do not communicate or intermingle with one another. On the one hand there is the sphere of order and work, in which belong the individuals who follow the bourgeois social patterns, seeking to live in an honest and dignified way, through labor and self-effort; on the other hand, lies the space of disorder and hustling [malandragem], in which are inserted those who reject the work ethics and live through conning, prostitution and everything related to a licentious life. In this context, the ethics of those who live through hustling and idleness prevails, for, in an excluding and competitive society, in which activism is a founding characteristic, in general those who resource to illegal means or marginal artifices to fulfill their goals succeed, whilst those who resign to their inferior condition end up defeated.

  16. "Ser Original: é ser verdadeiro e sincero": Belo Marques e a Música Negra

    OpenAIRE

    Dias, Michael

    2017-01-01

    O presente trabalho consiste em compreender a categoria “música negra” que Belo Marques utilizou em Música Negra – Estudo do folclore Tonga (1943) referindo-se a práticas expressivas no sul de Moçambique. Subjacente à formulação de tal categoria jaz uma complexa teia de relações e influências culturais e intelectuais, com a qual Belo Marques se relaciona, que importa descortinar de modo a apreender o sentido de “música negra”. É discutida a relação dialética, complexa, que se e...

  17. Nove Noites and Lourenço Marques: Criminal Mazes in the Contemporary Novel

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    Adenize Franco

    2014-10-01

    Full Text Available This article analyzes the characteristics of the crime novel in the contemporary fiction of Bernardo Carvalho, the Brazilian writer, and Francisco José Viegas, the Portuguese writer. The novels Nove Noites (2002 and Lourenço Marques (2002 are present as narratives marked by the characteristics of the detective genre in a movement that transgresses and subverts these elements.

  18. Application of quality improvement strategies in 389 European hospitals: results of the MARQuIS project

    NARCIS (Netherlands)

    Lombarts, M.J.M.H.; Rupp, I.; Vallejo, P.; Suñol, R.; Klazinga, N.S.

    2009-01-01

    Context: This study was part of the Methods of Assessing Response to Quality Improvement Strategies (MARQuIS) research project investigating the impact of quality improvement strategies on hospital care in various countries of the European Union (EU), in relation to specific needs of cross-border

  19. Papeles del Marqués del Risco referentes al Perú [Manuscrito

    OpenAIRE

    López Martínez, Juan Luis, , Marqués del Risco, (1644-1703

    2016-01-01

    Contenido:1. (h.1)5 hojas conteniendo dibujos a pluma, el 1 es una portada que representa un Templete, el 2&xBA una escena de la vida de Moisés, el 3 y el 4&xBA escudos de armas y el 5 retrato de un personaje ricamente vestido, que el autor el índice cree ser el Marqués del Risco.En el fol. 9 descripción de cómo ha de ser una lámina.2. (h.11) -Copia de comunicación de S.M. al Consejo de la Corona de Aragón para que le propongan al Marqués del Risco si hubiere alguna vacante correspondiente a ...

  20. Genealogías y molecular de los descendientes del Marquéz de Yavi

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    Alfaro Gómez, Emma Laura

    2007-01-01

    Full Text Available El título nobiliario de Marqués del Valle del Toxo, conocido comúnmente como Marqués de Yavi, era el más importante en el territorio del virreinato del Río de la Plata y se extendía por toda la Puna argentina y diversos municipios de Bolivia. Hubo cuatro marqueses desde su creación en 1708 por la Real Cédula emitida por Felipe V, rey de España, hasta su disolución en 1820 luego del fallecimiento del cuarto marqués Juan José Feliciano Fernández Campero. La abundancia de referencias históricas sobre el Marquesado de Yavi, el reciente interés político por recuperar la figura histórica del último Marqués sumado al encuentro social de sus descendientes ofreció la extraordinaria posibilidad de realizar una reconstrucción detallada de la genealogía de la familia Campero y relacionarla con el estudio molecular de sus descendientes. Se analizaron muestras de 30 individuos Campero a través de 6 microsatélites del cromosoma Y (DYS:19, 389a y b, 390,392,393 Se encontraron 8 linajes paternos bien definidos, 16 muestras comparten un linaje único, el segundo linaje congrega a 5 individuos, el tercero y cuarto linaje cuentan con 2 individuos cada uno y en 5 muestras se encontraron linajes individuales no relacionados con los anteriores. De estos resultados se interpreta que en la Genealogía Campero coexisten linajes diferentes, de los cuales uno de ellos, el mayoritario, probablemente sea el linaje fundador de esta genealogía. Se discuten estos resultados a la luz de la información genealógica e histórica.

  1. Envenimation mortelle par morsure de serpent chez une femme ...

    African Journals Online (AJOL)

    Les auteurs rapportent un cas de morsure de serpent au niveau de la face chez une femme enceinte, dont l'évolution a été marquée par l'installation d'un oedème cervico-facial nécessitant une trachéotomie en urgence, et une mort foetale in utero avec troubles de l'hémostase responsable du décès maternel dans un ...

  2. Neuropathie optique bilatérale par intoxication aigue au méthanol ...

    African Journals Online (AJOL)

    La consommation d'alcool frelaté, contenant du méthanol, est un phénomène de société au Cameroun. Nous rapportons deux cas d'intoxication cécitante au méthanol dont l'évolution a été marquée pour l'un par l'installation d'une cécité irréversible et pour l'autre par une récupération partielle de l'acuité visuelle. Ces cas ...

  3. Systematics and diversification of Anindobothrium Marques, Brooks & Lasso, 2001 (Eucestoda: Rhinebothriidea).

    Science.gov (United States)

    Trevisan, Bruna; Primon, Juliana F; Marques, Fernando P L

    2017-01-01

    Tapeworms of the genus Anindobothrium Marques, Brooks & Lasso, 2001 are found in both marine and Neotropical freshwater stingrays of the family Potamotrygonidae. The patterns of host association within the genus support the most recent hypothesis about the history of diversification of potamotrygonids, which suggests that the ancestor of freshwater lineages of the Potamotrygonidae colonized South American river systems through marine incursion events. Despite the relevance of the genus Anindobothrium to understand the history of colonization and diversification of potamotrygonids, no additional efforts were done to better investigate the phylogenetic relationship of this taxon with other lineages of cestodes since its erection. This study is a result of recent collecting efforts to sample members of the genus in marine and freshwater potamotrygonids that enabled the most extensive documentation of the fauna of Anindobothrium parasitizing species of Styracura de Carvalho, Loboda & da Silva, Potamotrygon schroederi Fernández-Yépez, P. orbignyi (Castelnau) and P. yepezi Castex & Castello from six different countries, representing the eastern Pacific Ocean, Caribbean Sea, and river basins in South America (Rio Negro, Orinoco, and Maracaibo). The newly collected material provided additional specimens for morphological studies and molecular samples for subsequent phylogenetic analyses that allowed us to address the phylogenetic position of Anindobothrium and provide molecular and morphological evidence to recognize two additional species for the genus. The taxonomic actions that followed our analyses included the proposition of a new family, Anindobothriidae fam. n., to accommodate the genus Anindobothrium in the order Rhinebothriidea Healy, Caira, Jensen, Webster & Littlewood, 2009 and the description of two new species-one from the eastern Pacific Ocean, A. carrioni sp. n., and the other from the Caribbean Sea, A. inexpectatum sp. n. In addition, we also present a

  4. Systematics and diversification of Anindobothrium Marques, Brooks & Lasso, 2001 (Eucestoda: Rhinebothriidea.

    Directory of Open Access Journals (Sweden)

    Bruna Trevisan

    Full Text Available Tapeworms of the genus Anindobothrium Marques, Brooks & Lasso, 2001 are found in both marine and Neotropical freshwater stingrays of the family Potamotrygonidae. The patterns of host association within the genus support the most recent hypothesis about the history of diversification of potamotrygonids, which suggests that the ancestor of freshwater lineages of the Potamotrygonidae colonized South American river systems through marine incursion events. Despite the relevance of the genus Anindobothrium to understand the history of colonization and diversification of potamotrygonids, no additional efforts were done to better investigate the phylogenetic relationship of this taxon with other lineages of cestodes since its erection. This study is a result of recent collecting efforts to sample members of the genus in marine and freshwater potamotrygonids that enabled the most extensive documentation of the fauna of Anindobothrium parasitizing species of Styracura de Carvalho, Loboda & da Silva, Potamotrygon schroederi Fernández-Yépez, P. orbignyi (Castelnau and P. yepezi Castex & Castello from six different countries, representing the eastern Pacific Ocean, Caribbean Sea, and river basins in South America (Rio Negro, Orinoco, and Maracaibo. The newly collected material provided additional specimens for morphological studies and molecular samples for subsequent phylogenetic analyses that allowed us to address the phylogenetic position of Anindobothrium and provide molecular and morphological evidence to recognize two additional species for the genus. The taxonomic actions that followed our analyses included the proposition of a new family, Anindobothriidae fam. n., to accommodate the genus Anindobothrium in the order Rhinebothriidea Healy, Caira, Jensen, Webster & Littlewood, 2009 and the description of two new species-one from the eastern Pacific Ocean, A. carrioni sp. n., and the other from the Caribbean Sea, A. inexpectatum sp. n. In addition, we

  5. La sérialité au cinéma : une stratégie de marque ?

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    Hélène Laurichesse

    2011-04-01

    Full Text Available Le phénomène sériel au cinéma peut s’analyser comme une orientation stratégique tournée vers la reproduction du succès. La production de suites conçues après le succès d’un film (sequels ou prequels, ou encore par des créations pensées dès l’origine sous forme de séries (adaptation de sagas littéraires par ex. illustre cette démarche. La notoriété pré-existante constitue la première composante d’une stratégie de marque qui va rendre possible à terme le développement d’une politique de fidélisation du public. L’étude du cas de la saga Twilight, série de livres déclinée en plusieurs épisodes filmiques, nous permettra d’analyser cette logique.The serial phenomenon in the movie industry can be analysed as a strategy focus on a success reproduction. Sequels or saga literary’s adaptations illustrate this approach. The pre-existing fame is the first component of a brand’s strategy which manages to develop a customer loyalty program. The study of the Twilight's saga case, a series of books turned into multiple film episodes will allow us to analyse this logic.

  6. Marked bengal pink. A dynamic test using marking doses; its value in jaundice diagnosis; Rose bengale marque. Epreuve dynamique a doses tracantes; sa valeur dans le diagnostic des icteres

    Energy Technology Data Exchange (ETDEWEB)

    Kellershohn, C.; Desgrez, A. [Commissariat a l' Energie Atomique, Service Frederic Joliot, Service de Biologie, Saclay (France).Centre d' Etudes Nucleaires; Caroli, J. [Hopital Saint Antoine, 75 - Paris (France); Delaloye, B. [Lausanne (Switzerland)

    1961-07-01

    The authors do a historic calling back of the differential study of jaundices thanks to the semi logarithmic gradients of expurgation of bromine sulphone-phthalein and thanks to the time of appearance of this material in the bile collected by duodenal tubing. They sum up likewise the foreign works which lead to take the place of this test, the outside count at the level of the head, the liver, and the abdomen after intravenous injection of a running dose of marked Rose Bengal. They specify the technical conditions of this test and report an experience which corroborate its value, its accuracy and the interest of its using in the different types of jaundices (hepatitis and obstructive types). (author) [French] Les auteurs font un rappel historique de l'etude differentielle des icteres grace aux pentes semi-logarithmiques d'epuration de la bromesulfonephtaleine et grace au temps d'apparition de cette substance dans la bile recueillie par tubage duodenal. Ils resument egalement les travaux etrangers qui tendent a substituer a cette epreuve le comptage externe au niveau de la tete, du foie et de l'abdomen, apres injection intraveineuse d'une dose tracante de rose bengale marque. Ils precisent les conditions techniques de cette epreuve et rapportent une experience qui confirme sa valeur, son exactitude et l'interet de son utilisation dans les differentes categories d'icteres par hepatite et par obstruction. (auteur)

  7. Cross-border care and healthcare quality improvement in Europe: the MARQuIS research project.

    Science.gov (United States)

    Suñol, R; Garel, P; Jacquerye, A

    2009-02-01

    Citizens are increasingly crossing borders within the European Union (EU). Europeans have always been free to travel to receive care abroad, but if they wished to benefit from their statutory social protection scheme, they were subject to their local or national legislation on social protection. This changed in 1991 with the European Court of Justice defining healthcare as a service, starting a debate on the right balance between different principles in European treaties: movement of persons, goods and services, versus the responsibility of member states to organise their healthcare systems. Simultaneously, cross-border cooperation has developed between member states. In this context, patient mobility has become a relevant issue on the EU's agenda. The EU funded a number of Scientific Support to Policies (SSP) activities within the Sixth Framework Programme, to provide the evidence needed by EU policy makers to deal with issues that European citizens face due to enhanced mobility in Europe. One SSP project "Methods of Assessing Response to Quality Improvement Strategies" (MARQuIS), focused on cross-border care. It aimed to assess the value of different quality strategies, and to provide information needed when: (1) countries contract care for patients moving across borders; and (2) individual hospitals review the design of their quality strategies. This article describes the European context related to healthcare, and its implications for cross-border healthcare in Europe. The background information demonstrates a need for further research and development in this area.

  8. Monumentality and public space in Lourenço Marques in the 1930 and 1940

    Directory of Open Access Journals (Sweden)

    Gerbert Verheij

    2012-01-01

    Full Text Available Lourenço Marques, actual Maputo (Mozambique, is subjected to a series of aesthetic interventions in its public space during the 1930s e 1940s. These seek to “monumentalize” and “portugalize” the city, responding to its recently acquired status as capital of the Colony. Two monuments appear as especially important and exemplary: the Padrão de Guerra, a lately built First War memorial (1935, and the monument to the hero of the “pacification campaigns” of the 1890s, Mouzinho de Albuquerque (1940. Around these monuments, a large number of commemorative and celebrative practices are developed. Such practices posit the monument as a “national allegory” and reproduce, in the context of a modern city, auratic and cult values. As such, they add an important dimension to the monument’s role in the authoritarian reformulation of the city’s public space as an “imperial” space, as well as in the putative hegemonization of the representations of the community imagined as a “Nation”. They allow, therefore, to approach the political-ideological use and utility of the monument within the organization of public space.

  9. A new species of Potamotrygonocestus Brooks & Thorson, 1976 (Eucestoda: Tetraphyllidea) from Plesiotrygon iwamae Rosa, Castello & Thorson (Mylliobatoidea: Potamotrygonidae) and a redescription of Potamotrygonocestus chaoi Marques, Brooks & Araujo, 2003.

    Science.gov (United States)

    Luchetti, N M; Marques, F P L; Charvet-Almeida, P

    2008-06-01

    Potamotrygonocestus Brooks & Thorson, 1976 is currently represented by six recognised species of tetraphyllidean cestodes inhabiting Neotropical freshwater stingrays. Potamotrygonid stingrays examined to date have included only a single specimen of Plesiotrygon iwamae. Only one species of tetraphyllidean, Potamotrygonocestus chaoi Marques, Brooks & Araujo, 2003, has been described from this host, and this description was based on limited material. New efforts to document the diversity in this host species resulted in the collection of eight additional specimens of P. iwamae, one of these from the upper Rio Solimões, at São Paulo de Olivença Amazonas during September, 2003, and seven from the Baía do Marajó, Pará, during November, 2003. The specimen from the upper Solimões was found to be infected with P. chaoi. Voucher material from this stingray was used for the redescription of this cestode, which is characterized by strobila 8.78-22.83 mm long and a great number of proglottides, 58-93; the new material provided strobilar length and proglottis counts for complete worms. Potamotrygonocestus marajoara n. sp. is the second species of this genus reported from Plesiotrygon iwamae, although it appears to be restricted to the lower Amazon. This new species resembles P. chaoi in possessing filitriches and blade-like spinitriches on the scolex, cephalic peduncle and cirrus, but differs from other species of the genus in the number of testes, which is 44 on average per proglottis, and by having apical sucker measuring 95-175 microm in length. Additional data on the distribution and morphology of the microtriches and a detailed description of the female reproductive system are also provided in this study.

  10. Contribution to the study of the biological properties of compounds labeled with radio-chromium {sup 51}Cr; Contribution a l'etude des proprietes biologiques des composes marques au radiochrome {sup 51}Cr

    Energy Technology Data Exchange (ETDEWEB)

    Ingrand, J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-07-15

    Among the radioisotopes commonly used in biology and medicine which are controlled Individually in the Radioelement Departement of the Saclay Nuclear Research Centre before being sent to the users, the author has chosen chromium 51 incorporated in inorganic salts or in organic substrates for a study of the biological properties of the compounds. In the first part, he has compared the pathways followed by the radioactive sodium chromate and chromic chloride mixed with blood or given to the whole animal, the object being to determine whether a reduction of hexavalent chromium occurs, both in vitro and in vivo. In the second part, the author has tried to show the validity of using, various substrates labeled with chromium 51, red cells, haemoglobin, plasma proteins and cytochrome c. The results obtained have contributed to underline the interest of using such compounds for biological applications. (author) [French] Parmi les radioisotopes d'utilisation courants en biologie et en medecine qui sont l'objet d'un controle particulier dans le Departement des Radioelements du Centre d'Etudes Nucleaires de Saclay avant leur diffusion aux utilisateurs, l'auteur a choisi le chrome 51 incorpore a des sels mineraux ou a des substrats organiques, afin d'en etudier les proprietes biologiques. Dans la premiere partie, il a compare le sort du chromate de sodium et du chlorure chromique radioactifs melanges a du sang ou administres a l'animal entier en s'efforcant de mettre en evidence une reduction du chrome hexavalent aussi bien in vitro qu'in vivo. Dans la deuxieme partie, il a cherche a etablir la validite de l'emploi de differents substrats marques au chrome 51, l'hematie, l'hemoglobine, les proteines plasmatiques et le cytochrome c. Les resultats obtenus ont permis de souligner le reel interet des applications biologiques des composes marques par le radioisotope. (auteur)

  11. Mucocèle sphénoïdale révélée par une exophtalmie | Lahiani | Pan ...

    African Journals Online (AJOL)

    La TDM du massif facial en coupe axiale a montré un processus expansif sphénoïdal et ethmoïdal postérieur droit qui comprime le nerf optique et souffle les parois osseuses. L'IRM du massif ... L'évolution était marquée par une régression incomplète des troubles visuels sans notion de récidive avec un recul de 36 mois.

  12. [Characteristics of induced labors at Marqués de Valdecilla University Hospital].

    Science.gov (United States)

    González-Maestro, Marta; Laurrieta-Saiz, Izaskun; García-González, Casandra; López-Mirones, Marta; Terán-Muñoz, Oihane; Alonso-Salcines, Alicia

    2017-01-03

    Induction of labor (IOL) is a common obstetric practice which has increased in recent decades. The main objective of this study is to describe the characteristics of induced labor at Marqués de Valdecilla University Hospital. A descriptive, retrospective study was performed between January 1st and April 30t h of 2014, by reviewing medical histories. A standardised registration has been used to collect data. There were a total of 376 IOL, the incidence was 35.1%. The most frequent indications were premature rupture of membranes (33.9%) and gestational age of 41 completed weeks or more (16.7%). Oxytocin was used to start 66.8% of the IOL. Of all births, 61.4% were spontaneous vaginal births and 25.3% caesarean sections. An increase in the rate of caesarean sections was observed in the group of gestational age of 41 completed weeks or more (27.3%) and hypertensive disorders of pregnancy (29.8%). The main cause of dystocia was suspected fetal compromise (36.4%). Adaptation to extrauterine life was within the parameters of normality in 94% of the newborn infants. Comparing the IOL with spontaneous labor, a decrease in spontaneous vaginal births and an increase in caesarean sections and operative vaginal births was observed in the inductions. This study provides relevant information to the professionals in the fields of gynaecology and obstetrics, and opens the way for future research to obtain results transferable to the clinical practice. Copyright © 2016 Elsevier España, S.L.U. All rights reserved.

  13. Par-tjek Manualen

    DEFF Research Database (Denmark)

    Trillingsgaard, Tea; Due, Mattias Stølen; Nørr Fentz, Hanne

    Par-tjek er et tilbud til par, der ønsker at styrke forholdet og forebygge vanskeligheder. Et Par-tjek består af et indledende online spørgeskema efterfulgt at to samtaler med en psykolog eller lignende vejleder. Ved den sidste samtale modtager parret en personlig feedback-rapport, som de kan...... arbejde videre med. Samtalerne kombinerer elementer af udredning, caseformulering, psykoedukation og parterapi. Par-tjek manualen er en vejledning til fagpersoner, der arbejder med Par-tjek....

  14. FooPar

    DEFF Research Database (Denmark)

    Hargreaves, F. P.; Merkle, D.

    2013-01-01

    We present FooPar, an extension for highly efficient Parallel Computing in the multi-paradigm programming language Scala. Scala offers concise and clean syntax and integrates functional programming features. Our framework FooPar combines these features with parallel computing techniques. FooPar......, results based on a empirical analysis on two supercomputers are given. We achieve close-to-optimal performance wrt. theoretical peak performance. Based on this result we conclude that FooPar allows programmers to fully access Scalas design features without suffering from performance drops when compared...

  15. Nonconsecutive Pars Interarticularis Defects.

    Science.gov (United States)

    Elgafy, Hossein; Hart, Ryan C; Tanios, Mina

    2015-12-01

    Lumbar spondylolysis is a well-recognized condition occurring in adolescents because of repetitive overuse in sports. Nonconsecutive spondylolysis involving the lumbar spine is rare. In contrast to single-level pars defects that respond well to conservative treatment, there is no consensus about the management of multiple-level pars fractures; a few reports indicated that conservative management is successful, and the majority acknowledged that surgery is often required. The current study presents a rare case of pars fracture involving nonconsecutive segments and discusses the management options. In this case report, we review the patient's history, clinical examination, radiologic findings, and management, as well as the relevant literature. An 18-year-old man presented to the clinic with worsening lower back pain related to nonconsecutive pars fractures at L2 and L5. After 6 months of conservative management, diagnostic computed tomography-guided pars block was used to localize the symptomatic level at L2, which was treated surgically; the L5 asymptomatic pars fracture did not require surgery. At the last follow-up 2 years after surgery, the patient was playing baseball and basketball, and denied any back pain. This article reports a case of rare nonconsecutive pars fractures. Conservative management for at least 6 months is recommended. Successful management depends on the choice of appropriate treatment for each level. Single-photon emission computed tomography scan, and computed tomography-guided pars block are valuable preoperative tools to identify the symptomatic level in such a case.

  16. Melanoma vectorized radiotherapy: preclinical evaluation of a new marker labelled by iodine 131; Radiotherapie vectorisee du melanome: evaluation preclinique d un nouveau vecteur marque par l iode 131

    Energy Technology Data Exchange (ETDEWEB)

    Bonnet, M.; Papon, J.; Miot-Noirault, E.; Madelmont, J.C.; Chezal, J.M.; Moins, N. [INSERM/UdA, EA4231, UMR 990, 63 - Clermont Ferrand (France); Mishellany, F.; Penault-Llorca, F.; Cayre, A. [laboratoire d anatomopathologie, CJP, 63 - Clermont-Ferrand (France)

    2010-05-15

    A significant anti tumoral effect of the internal vectorized radiotherapy using the compound {sup 131}I-ICF01012 is demonstrated on different models of melanomas, whatever be their aggressive potential, the quantity and localisation of the melanoma target. this effect is associated to an inhibition of the tumors cells dissemination towards lungs. The optimization of the protocol therapy is underway to get a maximum therapy efficiency associated to a toxicity controlled on non target-organs. (N.C.)

  17. Évaluation de l'application par l'Afrique du Sud d'une taxe d'accise ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Les maladies non transmissibles, qui contribuent de façon marquée à la charge de morbidité de l'Afrique du Sud sont largement causées par l'obésité et les maladies associées. La prévalence de l'obésité a augmenté de 30 % pendant la dernière décennie, tandis que la consommation d'aliments à haute teneur en énergie ...

  18. La mosaïque politique de la France : 15 cartes par canton pour comprendre les élections présidentielles 2002

    Directory of Open Access Journals (Sweden)

    Michel Bussi

    2002-04-01

    Full Text Available Analyser " à chaud " le comportement électoral des 4 000 cantons français... une première Le premier tour de l’élection présidentielle de 2002 est présenté par tous les spécialistes comme une élection de rupture. Cette rupture se marque non seulement par le score sans précédent atteint par le Front-national, mais également par le niveau de l’abstention, et l’éclatement de l’offre politique. Près d’un quart des suffrages exprimés se sont dirigés vers des candidats qui ont atteint moins de 5% ...

  19. suPAR

    DEFF Research Database (Denmark)

    Hodges, Gethin W; Bang, Casper N; Wachtell, Kristian

    2015-01-01

    The fundamental role of inflammation in cardiovascular disease (CVD) has prompted interest in numerous biomarkers that detect subclinical levels of inflammation. Soluble urokinase plasminogen activator receptor (suPAR) is a novel biomarker that correlates significantly with cardiovascular events...... and outperforms traditional markers of inflammation such as C-reactive protein (CRP) in prognosticating a range of CVDs. Furthermore, of particular interest is the suggestion that suPAR reflects a pathophysiological pathway more closely linked with subclinical organ damage than CRP. We provide the first...... comprehensive review of suPAR in CVD and explore its function and usefulness in predicting cardiovascular events....

  20. Isabel la Católica y el marqués de Cádiz, o la cortesía en la representación historiográfica del poder

    Directory of Open Access Journals (Sweden)

    Juan Luis CARRIAZO RUBIO

    2006-03-01

    Full Text Available On analyse ici la reconstruction, dans les chroniques, du processus complexe de soumission de la maison d’Arcos à la nouvelle monarchie qu’incarne la reine Isabelle. La présence d’une femme sur le trône de Castille fut, pour les chroniqueurs, l’occasion de condenser dans une série de scènes d’une grande intensité littéraire, le rapprochement compliqué, non sans négociations, qui fit de Rodrigo Ponce de León, l’adversaire redouté, un héros, voire une icône du projet politique des Rois Catholiques. Palencia, Valera, Pulgar ou encore Bernáldez, entre autres, proposent des versions parfois divergentes où ils attribuent, moyennant quelques écarts sensibles, un rôle plus ou moins important à Isabelle dans sa réconciliation avec le marquis. Mais au-delà des intérêts et des marques de loyauté des chroniqueurs, la présence même de la reine est un moyen de compléter le profil guerrier de ce héros de la frontière par l’évocation de manifestations de courtoisie des plus raffinées.Se analiza aquí la plasmación cronística del complejo proceso de sumisión de la Casa de Arcos a la nueva monarquía encarnada por la reina Isabel. No en vano, la presencia de una mujer en el trono de Castilla brindó a los cronistas la posibilidad de condensar en una serie de escenas de gran intensidad literaria el complicado acercamiento, no exento de negociaciones, que transformó a Rodrigo Ponce de León de adversario temido en héroe e incluso icono del proyecto político de los Reyes Católicos. Palencia, Valera, Pulgar o Bernáldez, entre otros, ofrecen relatos no siempre coincidentes que divergen de manera sensible al conceder a Isabel mayor o menor protagonismo en la reconciliación con el marqués. Pero más allá de los intereses y lealtades de los cronistas, la propia presencia de la reina les permite completar el perfil guerrero del héroe fronterizo con las más refinadas manifestaciones de cortesía.

  1. La metaphore est-elle la marque de l’insuffisance de l’esprit?

    Directory of Open Access Journals (Sweden)

    Isabelle Oliveira

    2010-01-01

    Full Text Available L’une des caractéristiques les plus communément reconnues à la discipline de la terminologie est sa conceptualité. Au contraire de l’opinion émise sur la métaphore, la terminologie s’efforce de travailler à partir de notions précises dont le sens doit être délimité et l’articulation à d’autres notions explicitée afin de bannir les ambiguïtés et les fausses évidences qu’elles véhiculent. Elle se distingue aussi du texte poétique caractérisé en particulier par la richesse des images et le jeu sur les signifiants. On comprend dès lors pourquoi la métaphore apparaît de prime abord comme une ennemie du champ de la terminologie qu’il faudrait bannir à tout prix. Cette figure de rhétorique consistant en un transport de sens risque à tout moment de rendre inopérant l’effort d’univocité et de rigueur du travail du terminologue. Mais la métaphore n’est pourtant pas absente des textes de spécialité. Il faut dès lors se demander si cette présence est « accidentelle”, contingente, ou si elle est incontournable et signifiante. Pour cela, il nous faudra étudier à la fois la place de la métaphore dans le texte de spécialité, et le discours des scientifiques portant sur elle. Mais une telle démarche nous laisse prisonnier de la dichotomie entre concept et métaphore et masque peutêtre une parenté inaperçue entre eux. Si le concept est habité par la métaphore, qu’en est-il du discours de spécialité? N’est-il pas réduit à n’être qu’un vaste poème qui ne s’avouerait pas comme tel? Est-elle seulement une figure de style ou aurait-elle un impact qui outrepasseraitsa modeste fonction discursive ? Il semble bien, même si le débat sur la métaphore et son usage est loin d’être clos. Notre théorie s’inscrit plutôt dans le sens d’une réévaluation du sens, d’une extension toujours plus grande de l’impact de ce trope qui, plus qu’une simple finalité esthétique, aurait une

  2. It's quicker "Par Avignon"!

    CERN Document Server

    2005-01-01

    For a few years, the CERN Library has been receiving books from the University of Hanover sent via Avignon, at least that's what it says on the envelope. Such a detour would mean that parcels were travelling 720 km more than the distance separating Geneva and Hanover, which would be a very strange state of affairs. The explanation lies in a spelling mistake. The sender has been stamping parcels with a stamp that says "Par Avignon prioritaire" (first-class via Avignon) instead of "Par Avion prioritaire" (First Class Air Mail), a source of much amusement to the librarians!

  3. Marquage magnétique interne : essais de tolérance par de jeunes saumons atlantiques (Salmo salar L.

    Directory of Open Access Journals (Sweden)

    DUMAS J.

    1982-10-01

    Full Text Available Les effets de l'implantation d'une marque magnétique "Metalimphy" * de 20 x 1,5 mm dans la cavité abdominale de jeunes saumons d'élevage sont comparés à ceux d'un marquage classique par ablation de la nageoire adipeuse. Les essais sont effectués sur des tacons de deuxième été et des pré-saumoneaux d'un an dans deux piscicultures de Bretagne et des Pyrénées-Atlantiques. Quel que soit le mode d'insertion pratiqué (ventral ou latéral cette technique n'a aucun effet significatif sur la survie et la croissance des poissons, qu'ils soient tacons (observés sur 194 jours ou pré-saumoneaux (observés sur 56 jours. La cicatrisation est rapide et ce marquage n'entrave pas la smoltification. L'emploi d'une poudre antibiotique cicatrisante ne semble pas nécessaire en conditions normales de pisciculture. L'insertion par voie ventrale s'avère préférable à l'insertion latérale ; elle n'entraîne pas de pertes de marques et se révèle plus facile à pratiquer.

  4. Identité de l'Autre, identité par l'Autre : la gestion du patrimoine culturel indien au nord-est du Mexique

    Directory of Open Access Journals (Sweden)

    Anath Ariel de Vidas

    2005-02-01

    Full Text Available La région de la Huastèque (État de Veracruz, dans les tropiques humides au nord-est du Mexique, est marquée par la présence de deux groupes ethniques, les Nahuas et les Teenek. Les Nahuas, descendants des Aztèques, se distinguent de leurs voisins teenek, par la langue, les coutumes, les habits, l'organisation sociale interne et, de manière générale, par leur attitude moins circonspecte à l'égard du monde extérieur. En revanche, les Teenek, descendants de la civilisation huastèque qui donna s...

  5. suPAR

    DEFF Research Database (Denmark)

    Eugen-Olsen, Jesper; Giamarellos-Bourboulis, Evangelos J

    2015-01-01

    promising biomarker is soluble urokinase-type plasminogen activator receptor (suPAR). Three studies in large populations of critically ill patients and patients admitted to the emergency department have shown that concentrations >12ng/mL can safely predict unfavourable outcome. This review presents...

  6. Gold-198 and rose bengal marked with iodine-131 in the diagnostic of hepatic vesicular affections; L'or{sup 198} et le rose bengale marque a l'iode{sup 131} dans le diagnostic des affections hepatovesiculaires

    Energy Technology Data Exchange (ETDEWEB)

    Manambelona Razafimalaza, J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1961-06-15

    Colloidal gold-198 makes it possible to obtain clear images of hepatic parenchyma; the examination can be repeated from different angles thus demonstrating the presence of pathologically inert regions, whether they be hydatic cysts, abscesses or neoplasia. The study of the disappearance curve for the colloid, together with a measurement of the blood volume, makes it possible also to calculate the hepatic flow. Using Rose Bengal marked with iodine-131, it is possible to obtain images of the liver and of the bile ducts, and to follow the elimination of the dye in the intestines. The simultaneous recording of the disappearance curves for the blood and of the appearance of the dye in the intestines constitutes an useful working test which is particularly sensible for evaluating the permeability of the bile ducts and, to a certain degree, the site of an obstruction. (author) [French] L'or colloidal-198 permet d'obtenir avec nettete des images du parenchyme hepatique; l'examen peut etre repete sous plusieurs incidences mettant ainsi en evidence les zones muettes pathologiques, qu'il s'agisse de kystes hydatiques, d'abces ou de neoplasies. L'etude de la courbe d'epuration du colloide completee par une mesure du volume sanguin offre en outre la possibilite de calculer le debit hepatique. A l'aide du rose bengale marque a l'iode-131, il est possible d'obtenir des images du foie et de la vesicule biliaire, et de suivre l'elimination intestinale du colorant. L'enregistrement simultane des courbes d'epuration sanguine et d'apparition intestinale constitue une epreuve fonctionnelle particulierement sensible pour apprecier la permeabilite des voies biliaires et dans une certaine mesure le site d'une obstruction. (auteur)

  7. Célébrer l'ordinaire : vues de Yeoville par ses habitants

    Directory of Open Access Journals (Sweden)

    Claire Bénit-Gbaffou

    2010-09-01

    Full Text Available Illustration 1 – The Ridge, Johannesburg, 2010Cette photographie, prise par un étudiant de troisième année à la School of Architecture and Planning de Wits dans le cadre du projet de recherche/action Yeoville Studio, représente « The Ridge » à Johannesburg. The Ridge marque la frontière entre le quartier de Yeoville et les quartiers denses de Berea et Hillbrow (arrière plan de la photo dans l’inner city. Cet espace interstitiel est un lieu privilégié pour les pratiques religieuses des réside...

  8. Envenimation mortelle par morsure de serpent chez une femme enceinte

    Directory of Open Access Journals (Sweden)

    Abdelkarim Shimi

    2011-02-01

    Full Text Available En Afrique, la prise en charge des envenimations vipérines demeure un problème majeur de santé publique avec un taux de mortalité qui reste élevé.La survenue d’une envenimation vipérine au cours de la grossesse est un événement rare, et grave du fait des conséquences maternelles et fatales qui en découlent. Les auteurs rapportent un cas de morsure de serpent au niveau de la face chez une femme enceinte, dont l’évolution a été marquée par l’installation d’un œdème cervico-facial nécessitant une trachéotomie en urgence, et une mort fatale in utero avec troubles de l’hémostase responsable du décès maternel dans un tableau de choc hémorragique.

  9. PAR Loop Schedule Review

    Energy Technology Data Exchange (ETDEWEB)

    Schaffer, Jr.; W.F.

    1958-04-30

    The schedule for the installation of the PAR slurry loop experiment in the South Facility of the ORR has been reviewed and revised. The design, fabrications and Installation is approximately two weeks behind schedule at this time due to many factors; however, indications are that this time can be made up. Design is estimated to be 75% complete, fabrication 32% complete and installation 12% complete.

  10. Algunas gestiones de mediación del Marqués de Villalobar durante la I Guerra Mundial

    Directory of Open Access Journals (Sweden)

    Álvaro Lozano Cutanda

    2005-01-01

    Full Text Available En este artículo deseo recordar a una figura de primer orden de la diplomacia española: Don Rodrigo Saavedra y Vinent, Marqués de Villalobar. Se trató de un personaje clave dentro la política exterior del Rey Alfonso XIII. Su amistad y vínculos con la Corona, hicieron de él un defensor de la misma en sus puestos diplomáticos y encarnó como nadie la política exterior que quiso llevar a cabo el Rey Alfonso XIII. El gran mérito del Embajador Villalobar fue el de superar una grave incapacidad física y con una gran fuerza de voluntad haber llegado a representar a España. Esa sobrehumana superación personal, unida a su fuerte sentimiento monárquico, aristocrático y humanitario, fueron las constantes en su vida y su profesión.In this article I would like to shed light on an outstanding Spanihs diplomat: Don Rodrigo Saavedra y Vinent, Marquis of Villalobar. He was a key figure in the foreign policy of King Alfonso XIII. His friendship and links with the Spanish royalty made him a strong supporter of the Crown in his diplomatic posts and he embodied like no one else the foreign policy of his King. The greatest merit of Ambassador Villalobar, was to overeóme a big physical handicap, and through sheer will power, to become an ambassador. Without doubts, overcoming that handicap profoundly marked his personality during his entire Ufe. That immense will power and his strong monarchic, aristocratic and humanitarian feelings, were the most outstanding features of his Ufe and work.

  11. Tempo e melancolia: república, modernidade e cidadania negra nos contos de Astolfo Marques (1876-1918 Time and melancholy: Republic, modernity and black citizenship in the short story of Astolfo Marques (1876-1918

    Directory of Open Access Journals (Sweden)

    Matheus Gato de Jesus

    2012-01-01

    Full Text Available A história da Proclamação da República na periferia setentrional do Império brasileiro (1822-1889, assim como as especificidades de suas consequências culturais na região, ainda carece de análises mais detalhadas. No Maranhão de fin de siècle, a crise econômica do sistema agroexportador, somada à crise política do trabalho escravo, legou às primeiras décadas do regime republicano uma intensa luta social em torno da definição dos limites da cidadania, na qual a subalternização racial da população negra é um ponto decisivo. O presente trabalho é uma aproximação desses impasses através da obra ficcional do escritor negro maranhense Raul Astolfo Marques (1876-1918. Filho de uma cafusa livre, o autor é fruto da geração de descendentes escravos que se debateu com a fragilidade da liberdade conquistada na Abolição e do formalismo da igualdade outorgada em 15 de Novembro de 1889. Uma parte significativa da sua literatura poderia ser entendida como uma tentativa sistemática de interpretar essas esperanças e frustrações. O tema da República comparece ao longo de toda a sua trajetória intelectual em textos como "Abnegação" (1901, "A última sessão" (1903, "O discurso do Fabrício" (1903, "A opinião da Euzébia" (1904, "De coroa e barrete" (1908 e "Reis republicanos" (1916. Resta dizer que A nova aurora (1913, seu único romance, é inteiramente dedicado à descrição dos primeiros meses de implantação do regime republicano. Na primeira parte do ensaio indagamos quais "versões da história" e episódios significativos orientam a reconstrução ficcional das consequências do 15 de Novembro na periferia do Brasil, focalizando o conto "O discurso do Fabrício" e a crônica "A última sessão". No segundo momento, abordamos o tratamento literário dado pelo autor ao problema da política pública republicana no governo Rodrigues Alves (1902-1906, período auge da ideologia sanitarista no país e com tremendo impacto

  12. Parálisis cerebral :

    OpenAIRE

    Aguado Navarro, Ana Belén

    2013-01-01

    Se aborda el tema de la parálisis cerebral definiendo qué es, clasificando los tipos de parálisis dependiendo de la afectación y las características principales. Se explican algunos de sus tratamientos, se dan sistemas alternativos y/o aumentativos de comunicación para un alumno con PC (parálisis cerebral).

  13. Art and politics in the former "Portuguese Colonial Empire". The monument to Mouzinho de Albuquerque in Lourenço Marques

    Directory of Open Access Journals (Sweden)

    Verheij, Gerbert

    2013-01-01

    Full Text Available In 1940, a monument to Mouzinho de Albuquerque is inaugurated in the former capital of the Portuguese Colony of Mozambique, Lourenço Marques. The result of a lenghty commissioning process, this homage to one of the main heroes of the colonial pantheon becomes a center-piece in the many political rituals which had become common-place during the 1930s. The concepts of aura and cult value are used to analyse its "traditionalist" aesthetics and the role it played in political spectacles, arguing that it obeys a strategy of representation of the public space as "Empire".

  14. El real decreto y la circular del Marqués de Orovio de 1875: repercusiones en el claustro universitario y la prensa de Sevilla

    Directory of Open Access Journals (Sweden)

    María Nieves GÓMEZ GARCÍA

    2010-03-01

    Full Text Available RESUMEN: La segunda «cuestión universitaria», provocada por el Real Decreto de 26 de febrero de 1875, y por la Circular que, firmada por el mismo Marqués de Orovio se mandaba a los rectores de las Universidades, nos enfrenta a uno de los problemas más conflictivos para la historia de la educación española del siglo XIX, como fue sin duda el problema de la libertad de enseñanza.

  15. Os encantos do arquivo e os trabalhos do historiador: reflexões a partir da coleção Marquês de Valença

    Directory of Open Access Journals (Sweden)

    Eide Sandra Azev\\u00EAdo Abr\\u00EAu

    2011-01-01

    Full Text Available In this article reflections will be developed about issues concerning to the relations between subject and object of historical knowledge, from the reading experience and cataloging the documents of Coleção Marquês de Valença, of Museu Paulista. Through exploration of versions of Estevão Ribeiro de Rezende's biography made by his son Estevão Ribeiro de Souza Rezende, elements are presented in order to think about different options of historical narrative, and its implications on the relation established by the historian with the sources, men of the past and readers of his own time.

  16. Las representaciones de Don Gaspar de Haro y Guzmán, VII Marqués del Carpio: retratos, alegorías y emblemas

    Directory of Open Access Journals (Sweden)

    López-Fanjul y Díez del Corral, María

    2013-12-01

    Full Text Available The author studies various portraits of Don Gaspar de Haro y Guzmán, 7th Marquis of Carpio, and analyses the strong links that they had with his interest in drawing, emblems, allegories, and the search for fame through books.Este artículo está dedicado a estudiar una serie de retratos de Don Gaspar de Haro y Guzmán, VII Marqués del Carpio, especialmente importantes por su relación con el dibujo, las alegorías, los emblemas y la búsqueda de la fama a través de los libros.

  17. Brulures par Diluant

    Science.gov (United States)

    Benbrahim, A.; Jerrah, H.; Diouri, M.; Bahechar, N.; Boukind, E.H.

    2009-01-01

    Summary La flamme de diluant est une cause non rare de brûlure dans le contexte marocain. Nous avons jugé intéressant de faire une étude épidémiologique sur la brûlure par flamme de diluant (BFD) au centre national des brûlés (CNB) du CHU Ibn-Rochd de Casablanca. Ce travail a été réalisé sur une période de 10 mois (septembre 2007/juin 2008). Le but du travail est de montrer les caractéristiques de ce type de brûlures pour les prévenir et ce par l'information sur le diluant, produit causant ces brûlures, et ses différents dangers, la brûlure notamment. Durant cette période, nous avons colligé 17 cas de BFD sur un total de 356 patients admis au CNB pour brûlures aiguës toute étiologie confondue. La moyenne d'age des patients concernés est de 32 ans. Ils sont presque tous de sexe masculin (16 hommes/1 femme) et ont des antécédents de toxicomanie et/ou de délinquance. Tous nos patients sont de bas niveau socio-économique et habitent dans des bidonvilles pour la plupart. La brûlure est souvent secondaire à une agression dans la rue (92% des cas). Concernant les caractéristiques de la brûlure, la surface cutanée brûlée moyenne est de 23%; elle est souvent profonde et siège surtout au niveau des membres supérieurs et du tronc. PMID:21991179

  18. Insights into ParB spreading from the complex structure of Spo0J and parS.

    Science.gov (United States)

    Chen, Bo-Wei; Lin, Ming-Hsing; Chu, Chen-Hsi; Hsu, Chia-En; Sun, Yuh-Ju

    2015-05-26

    Spo0J (stage 0 sporulation protein J, a member of the ParB superfamily) is an essential component of the ParABS (partition system of ParA, ParB, and parS)-related bacterial chromosome segregation system. ParB (partition protein B) and its regulatory protein, ParA, act cooperatively through parS (partition S) DNA to facilitate chromosome segregation. ParB binds to chromosomal DNA at specific parS sites as well as the neighboring nonspecific DNA sites. Various ParB molecules can associate together and spread along the chromosomal DNA. ParB oligomer and parS DNA interact together to form a high-order nucleoprotein that is required for the loading of the structural maintenance of chromosomes proteins onto the chromosome for chromosomal DNA condensation. In this report, we characterized the binding of parS and Spo0J from Helicobacter pylori (HpSpo0J) and solved the crystal structure of the C-terminal domain truncated protein (Ct-HpSpo0J)-parS complex. Ct-HpSpo0J folds into an elongated structure that includes a flexible N-terminal domain for protein-protein interaction and a conserved DNA-binding domain for parS binding. Two Ct-HpSpo0J molecules bind with one parS. Ct-HpSpo0J interacts vertically and horizontally with its neighbors through the N-terminal domain to form an oligomer. These adjacent and transverse interactions are accomplished via a highly conserved arginine patch: RRLR. These interactions might be needed for molecular assembly of a high-order nucleoprotein complex and for ParB spreading. A structural model for ParB spreading and chromosomal DNA condensation that lead to chromosome segregation is proposed.

  19. Las investigaciones del Marqués de Cerralbo en el «Cerro Villar» de Monreal de Ariza : Arcobriga

    Directory of Open Access Journals (Sweden)

    Carmen Jiménez Sanz

    1998-01-01

    Full Text Available Se presenta un avance de los trabajos que se están realizando sobre la aportación del xvii Marqués de Cerralbo a la disciplina arqueológica, orientados al conocimiento de la Historia de la Arqueología española en las primeras décadas del siglo xx. Se revisa el caso del yacimiento romano de Arcobriga desde las circunstancias que motivaron su descubrimiento, objetivos de las investigaciones, metodología, desarrollo de los trabajos de campo y gabinete, intervención de otros especialistas, etc., partiendo de una breve introducción a la figura del Marqués de Cerralbo.We introduce an advance ofour work about XVII Marquis of Cerralbo and his contribution to the spanish Archaeology History, in the first decades of this century. Following a brief history about his Ufe, you will find a review of the case of Arcobriga's román site: circumstances that involved its discovery, research objectives, methodology, development of works, contributions of any other specialists and so on.

  20. Plasticidade de glândulas tegumentares abdominais em Monoeca xanthopyga Harter-Marques, Cunha & Moure (Hymenoptera, Apidae, Tapinotaspidini

    Directory of Open Access Journals (Sweden)

    Camila Gonçalves dos Santos

    2004-06-01

    Full Text Available Glândulas tegumentares são amplamente conhecidas em abelhas sociais e responsáveis por diversas funções nestes insetos. Entretanto, pouco se conhece sobre estas glândulas em abelhas solitárias. Monoeca xanthopyga é uma espécie de abelha solitária que exibe comportamento peculiar nas estratégias de acasalamento e no processo de nidificação. Este estudo visa verificar a ocorrência de glândulas tegumentares abdominais e a natureza química de produtos secretados por machos e fêmeas de M. xanthopyga, em dois momentos do ciclo vital: recém-emergidas e em período de nidificação. O material foi estudado utilizando-se microscopia óptica e eletrônica de varredura e histoquímica. Machos e fêmeas recém-emergidas e em período de nidificação apresentam glândulas tegumentares nos tergos III ao VII, entretanto diferem no tipo e na localização. Nos esternos de fêmeas recém-emergidas, as glândulas estão ausentes, já nas fêmeas em período de nidificação estas glândulas estão presentes nos esternos IV ao VI. Nos machos, as glândulas tegumentares estão presentes nos esternos IV ao VI. A análise histoquímica das glândulas tegumentares de machos e fêmeas sugere a presença de produtos de natureza lipídica, possivelmente envolvidos na comunicação relacionada ao comportamento sexual.Plasticity of abdominal tegumentar glands in Monoeca xanthopyga Harter-Marques, Cunha & Moure (Hymenoptera, Apidae, Tapinotaspidini. Tegumentar glands are highly known in social bees and are responsible for a diversity of functions in these insects. However, little is known about these glands in solitary bees. Monoeca xanthopyga is a solitary bee species that shows a singular behavior of mating and nesting. This study is to verify the occurrence of abdominal tegumentar glands and the chemical nature of the products secreted by males and females of M. xanthopyga, at two moments of the vital cycle: just emerged and at the nesting period. The

  1. Visualization of the macula during elective pars plana vitrectomy in the presence of a dual-optic accommodating intraocular lens.

    Science.gov (United States)

    Marques, Eduardo F; Ferreira, Tiago B; Castanheira-Dinis, António

    2014-05-01

    We describe the case of a 69-year-old patient who had pars plana vitrectomy (PPV) for epiretinal membrane removal 18 months after cataract surgery with implantation of a dual-optic accommodating IOL (Synchrony). During PPV, visualization of the macula was perfect, and the postoperative visual outcome was excellent. To our knowledge, this is the first report of retinal visualization during PPV in the presence of a dual-optic accommodating IOL. When choosing a presbyopia-correcting IOL, surgeons should consider the advantage of macula visualization with an IOL with monofocal optics. Dr. Marques is a consultant to Abbott Medical Optics, Inc. No other author has a financial or proprietary interest in any material or method mentioned. Copyright © 2014 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.

  2. Datation par thermoluminescence

    Directory of Open Access Journals (Sweden)

    1976-01-01

    Full Text Available Depuis 1953, de nombreux chercheurs se sont intéressés à la datation par thermoluminescence de minéraux anciennement brûlés ou cuits. Dans ce travail, après avoir rappelé quelques principes physiques de la thermoluminescence, on présente cette méthode de datation en mettant l'accent sur le mécanisme thermoluminescent dans une poterie. Ainsi la dose d'irradiation reçue par le matériau étant proportionnelle au temps écoulé depuis le 'zéro archéologique', il est possible de déterminer 'la dose archéologique' et d'en déduire l'âge de l'échantillon après avoir calculé la dose d'irradiation annuelle. La réalisation pratique d'un tel ensemble de mesure est cependant très ardue. Dans un prochain article, E. A. Decamps et A. Roman montreront des résultats relatifs à la thermoluminescence d'échantillons de quartz naturels, purs et dopés et la mise au point d'une nouvelle méthode de datation. Desde 1953, muchos investigadores se han interesado en la datación por termoluminiscencia de minerales antiguamente quemados o cocidos. Dentro de este trabajo, luego de haber recordado algunos principios físicos de la termoluminiscencia, se presenta este método de datación poniendo mayor atención en el mecanismo termoluminescente en una vasija de metal o de barro. Siendo proporcional la dosis de irradiación recibida al tiempo transcurrido desde el 'cero arqueológico', es posible determinar 'la dosis arqueológica', y deducir la edad de la muestra luego de haber calculado la dosis de irradiación anual. La realización práctica de un trabajo de tal dimensión es sin embargo muy ardua. En un próximo artículo, E. A. Decamps y A. Román presentarán los resultados relativos a la termoluminiscencia de muestras de cuarzo naturales, puras y dopadas y la elaboración de un nuevo método de datación. Since 1953, a number of scientists have been concerned with the use of thermoluminescence for the dating of burned or fired minerals

  3. Elusloom lennukiga puhkusele / Inge Parring

    Index Scriptorium Estoniae

    Parring, Inge

    2003-01-01

    Ilmunud ka: Delovõje Vedomosti 1. okt. lk. 13. Air Cargo Estonia/ACE Logisticsi müügijuht Inge Parring tutvustab elusloomade transpordivõimalusi. Vt. samas: Loomade transportimiseks vajalikud dokumendid

  4. suPAR: the molecular crystal ball

    DEFF Research Database (Denmark)

    Thunø, Maria; Macho, Betina; Eugen-Olsen, Jesper

    2009-01-01

    soluble urokinase Plasminogen Activator Receptor (suPAR) levels reflect inflammation and elevated suPAR levels are found in several infectious diseases and cancer. suPAR exists in three forms; suPAR(I-III), suPAR(II-III) and suPAR(I) which show different properties due to structural differences. ...... in inflammation and pathogenic processes. We focus on the molecular mechanisms of the suPAR fragments and the link to the inflammatory process,as this could lead to medical applications in infectious and pathological conditions........ Studies suggest that full-length suPAR is a regulator of uPAR/uPA by actingas uPA-scavenger, whereas the cleaved suPAR(II-III) act as a chemotactic agent promoting the immune response via the SRSRY sequence in the linker-region. This review focus on the various suPAR fragments and their involvement...

  5. Immunopathogenic Background of Pars Planitis.

    Science.gov (United States)

    Przeździecka-Dołyk, Joanna; Węgrzyn, Agnieszka; Turno-Kręcicka, Anna; Misiuk-Hojło, Marta

    2016-04-01

    Pars planitis is defined as an intermediate uveitis of unknown background of systemic disease with characteristic formations such as vitreous snowballs, snowbanks and changes in peripheral retina. The incidence of pars planitis varies 2.4-15.4 % of the uveitis patients. The pathogenesis of the disease is to be determined in future. Clinical and histopathological findings suggest an autoimmune etiology, most likely as a reaction to endogenous antigen of unknown source, with T cells predominant in both vitreous and pars plana infiltrations. T cells subsets play an important role as a memory-effector peripheral cell. Snowbanks are formed as an effect of post inflammatory glial proliferation of fibrous astrocytes. There is also a genetic predisposition for pars planitis by human leukocyte antigen and several other genes. A coexistence of multiple sclerosis and optic neuritis has been described in numerous studies. Epiretinal membrane, cataract, cystoid macular edema, retinal detachment, retinal vasculitis, neovascularization, vitreous peripheral traction, peripheral hole formation, vitreous hemorrhage, disc edema are common complications observed in pars planitis. There is a need to expand the knowledge of the pathogenic and immunologic background of the pars planitis to create an accurate pharmacological treatment.

  6. Sentir o sentido: a experiência do código nos “homeóstatos” de José-Alberto Marques

    Directory of Open Access Journals (Sweden)

    Manuel Portela

    2016-09-01

    Full Text Available http://dx.doi.org/10.5007/1807-9288.2016v12n1p69 Este breve ensaio analisa a série de poemas Homeóstatos de José-Alberto Marques como motores textuais. Ao tornar o código numa componente do texto, os Homeóstatos tipificam o poema experimental como uma investigação sobre a linguagem, a escrita e a leitura. A espacialização do texto na página é ativada de modo a produzir efeitos gerativos, combinatórios e ideogramáticos. Simultaneamente textos e instruções para gerar textos, os Homeóstatos constituem um exemplo de uma poética informacional que tira partido da dinâmica entre poema e sistema da língua para explorar os atos de escrita e leitura como processamento de sinais.

  7. Un modèle intégrateur du capital-client de la marque : une perspective psycho-cognitive

    OpenAIRE

    Czellar, Sandor; Denis, Jean-Emile

    2001-01-01

    L'approche psycho-cognitive du capital-client est exposée après avoir examiné les différents courants de recherche sur la valeur de la marque. Celle-ci constitue actuellement le paradigme dominant. Sur la base des travaux antérieurs, un modèle intégrateur des antécédents et des conséquences du capital-client est proposé. Ce modèle englobe l'ensemble du processus de création du capital-client, de la conception d'une stratégie marketing jusqu'aux conséquences à long terme du capital-client. La ...

  8. Les Brulures Electriques par Haut Voltage - A Propos de 10 Cas

    Science.gov (United States)

    Belmir, R.; Fejjal, N.; El Omari, M.; El Mazouz, S.; Gharib, N.; Abassi, A.; Belmahi, A.

    2008-01-01

    Summary Les accidents électriques par haute tension (AEHT) provoquent des brûlures profondes par effet Joule le long des axes vasculo-nerveux entre les points d'entrée et de sortie, qui sont le siège de lésions délabrantes. Les Auteurs rapportent une série de dix cas d'AEHT admis au service de chirurgie réparatrice et de brûlés de l'Hôpital Ibn Sina de Rabat à travers laquelle ils étudient les caractéristiques épidémiologiques, cliniques et thérapeutiques. Tous les patients étaient des adultes de sexe masculin dont l'âge moyen était de 31 ans. Dans 70% des cas, ces brûlures étaient secondaires à un contact avec les distributeurs d'électricité avec une surface brûlée inférieure à 20%. Le traitement des lésions électrothermiques a nécessité des interventions itératives avec amputation des segments de membres nécrosés dans 70% des cas, dont les suites étaient marquées par des séquelles fonctionnelles invalidantes. La prévention des AEHT, en particulier pour les accidents du travail au sein des professions exposées, reste fondamentale. PMID:21991124

  9. Designing Tone Reservation PAR Reduction

    Directory of Open Access Journals (Sweden)

    Johansson Albin

    2006-01-01

    Full Text Available Tone reservation peak-to-average (PAR ratio reduction is an established area when it comes to bringing down signal peaks in multicarrier (DMT or OFDM systems. When designing such a system, some questions often arise about PAR reduction. Is it worth the effort? How much can it give? How much does it give depending on the parameter choices? With this paper, we attempt to answer these questions without resolving to extensive simulations for every system and every parameter choice. From a specification of the allowed spectrum, for instance prescribed by a standard, including a PSD-mask and a number of tones, we analytically predict achievable PAR levels, and thus implicitly suggest parameter choices. We use the ADSL2 and ADSL2+ systems as design examples.

  10. Une terreur par l’image

    Directory of Open Access Journals (Sweden)

    Annie DULONG

    2011-09-01

    Full Text Available Si d’autres événements — Hiroshima, la découverte des camps de concentration, la chute du mur de Berlin — ont, au cours du XXe siècle, marqué suffisamment l’imaginaire pour trouver leur réponse dans des œuvres d’art, le 11 septembre 2001 pose la question de la fictionnalisation autrement : il ne s’agit plus tant de combler les manques de la représentation, liés à l’absence d’images ou au délai dans leur dévoilement, mais bien de composer avec l’omniprésence d’une représentation martelée à la télévision et sur Internet. Que cette représentation soit partielle, tronquée et qu’il lui manque la présence des corps, ces grands absents des photographies et des images tournées par les journalistes, ne change rien au fait que c’est avec le trop-plein que les artistes doivent maintenant négocier.Cet article s’intéresse aux romans qui traitent l’événement de front au lieu de l’aborder sur un mode mineur, et a pour objectif de tracer un portrait de la représentation des médias dans les romans du 11 septembre 2001. Ces romans, outre le fait qu’ils traitent des événements de New York, ont comme point commun un personnage, ou décor : les médias, représentés par la télévision, l’Internet, les photographies de presse, interviennent dans les récits d’une manière significative et témoignent de la force brute des images. À travers une étude de certains des mécanismes à l’œuvre dans The Writing on the Wall (Lynne Sharon Schwartz, Extremely Loud and Incredibly Close (Jonathan Safran Foer, Falling Man (Don DeLillo et A Disorder Peculiar to the Country (Ken Kalfus, il s’agira de proposer des réponses à ces quelques questions : quel rôle les médias jouent-ils, et comment participent-ils au récit ? Quelle pression les images exercent-elles sur les personnages ? Que révèlent les personnages enfants dans leur rapport aux médias ? Quelle critique, finalement, les romans

  11. Increased ParB level affects expression of stress response, adaptation and virulence operons and potentiates repression of promoters adjacent to the high affinity binding sites parS3 and parS4 in Pseudomonas aeruginosa.

    Directory of Open Access Journals (Sweden)

    Adam Kawalek

    Full Text Available Similarly to its homologs in other bacteria, Pseudomonas aeruginosa partitioning protein ParB facilitates segregation of newly replicated chromosomes. Lack of ParB is not lethal but results in increased frequency of anucleate cells production, longer division time, cell elongation, altered colony morphology and defective swarming and swimming motility. Unlike in other bacteria, inactivation of parB leads to major changes of the transcriptome, suggesting that, directly or indirectly, ParB plays a role in regulation of gene expression in this organism. ParB overproduction affects growth rate, cell division and motility in a similar way as ParB deficiency. To identify primary ParB targets, here we analysed the impact of a slight increase in ParB level on P. aeruginosa transcriptome. ParB excess, which does not cause changes in growth rate and chromosome segregation, significantly alters the expression of 176 loci. Most notably, the mRNA level of genes adjacent to high affinity ParB binding sites parS1-4 close to oriC is reduced. Conversely, in cells lacking either parB or functional parS sequences the orfs adjacent to parS3 and parS4 are upregulated, indicating that direct ParB- parS3/parS4 interactions repress the transcription in this region. In addition, increased ParB level brings about repression or activation of numerous genes including several transcriptional regulators involved in SOS response, virulence and adaptation. Overall, our data support the role of partitioning protein ParB as a transcriptional regulator in Pseudomonas aeruginosa.

  12. A trajetória profissional de Flora Costa Marques: ex-aluna da Escola de Enfermagem Aurora de Afonso Costa (1950-1983 La trayectoria profesional de Flora Costa Marques: ex-alumna de la Escuela de Enfermería Aurora de Afonso Costa (1950-1983 The professional trajectory of Flora Costa Marques: former student of the Aurora de Afonso Costa School of Nursing (1950-1983

    Directory of Open Access Journals (Sweden)

    Zenith Rosa Silvino

    2005-12-01

    Full Text Available A pesquisa de cunho histórico social descreve a trajetória de Flora Costa Marques, uma ex-aluna da antiga Escola de Enfermagem do Estado do Rio de Janeiro, atual Escola de Enfermagem Aurora de Afonso Costa, na Saúde Pública do município de Niterói, destacando as contribuições da Escola à formação de enfermeiras para atuação no campo da saúde pública. O recorte temporal é de 1950, ano de formatura da segunda turma do curso de graduação em enfermagem da referida escola, a 1983, ano da aposentadoria da enfermeira. Utilizamos o método história de vida e como técnica de coleta a entrevista aberta. Ao analisarmos a trajetória da enfermeira, observamos que as atividades desenvolvidas na época remetem aos Programas atuais: Programa Saúde da Família, Programa Médico da Família e Programa Agentes Comunitários de Saúde. Assim, as contribuições da Escola, com ênfase na área da saúde pública, consolidaram-se através do preparo de enfermeiras que lidam com as mais variadas adversidades no campo da saúde.La investigación de enfoque histórico y social describe la trayectoria de Flora Costa Marques, una ex-alumna de la antigua Escuela de Enfermería del Estado de Rio de Janeiro - Brasil, actual Escuela de Enfermería Aurora de Afonso Costa, en la Salud Pública del municipio de Niteroi, destacando las contribuciones de la Escuela a la formación de enfermeras para actuación en el campo de la salud pública. El tiempo va desde 1950, año de la licenciatura de la segunda turma del curso de graduación de enfermeros de la referida escuela, hasta 1983, año de la jubilación de la enfermera. Utilizamos el método historia de vida y como técnica de colecta la entrevista abierta. Al analizar la trayectoria de la enfermera, se ha observado que las actividades a la época remiten a los Programas actuales: Programa Salud de la Familia, Programa Médico de Familia y Programa Agentes Comunitarios de Salud. Así, las contribuciones

  13. L'influence du capital marque sur le risque perçu de la substitution d'un médicament de marque par un médicament générique : proposition d'un modèle

    OpenAIRE

    Ferchakhi, Widiane; Cegarra, Jean-Jack

    2014-01-01

    International audience; Face generics laboratories that develop important communication campaigns to increase brand awareness and build a reputation, innovative laboratories are experiencing growing difficulties to extend the life of their original drugs. In this context, brands' equity (brand of innovative drug vs brand of generic) may be considered as a moderator of the risk perceived face of substitution. Based on a review of the literature on the determinants to the purchase of generic dr...

  14. DETERMINATION DU NIVEAU DE CONTAMINATION PAR LES METAUX LOURDS DANS LES POISSONS D'IMPORTATION CAS DE LA CREVETTE ET FILET DE MERLAN

    OpenAIRE

    MOKRANI, Mohammed

    2015-01-01

    L'objectif de cette étude est l'évaluation des niveaux de contamination en métaux lourds (Pb, Cd, Cr, Zn, Cu) dans trois produits congelés transformés, importés de Chine et vendus en Algérie en 2014. Il s'agit de deux marques de crevettes rouges décortiquées et du filet de merlan. Après minéralisation des échantillons, l'analyse des éléments trace métalliques (ETM) était mesurée par l'appareil de la spectrophotométrie d'absorption atomique à flamme du laboratoire « d'Al Zinc » Gha...

  15. Hospital mortality prognostication in sepsis using the new biomarkers suPAR and proADM in a single determination on ICU admission.

    Science.gov (United States)

    Suberviola, B; Castellanos-Ortega, A; Ruiz Ruiz, A; Lopez-Hoyos, M; Santibañez, M

    2013-11-01

    The soluble form of the urokinase-type plasminogen activator receptor (suPAR) and proadrenomedullin (proADM) are two new and promising sepsis biomarkers. We assessed the prognostic value of a single determination of proADM and suPAR, comparing them with C-reactive protein (CRP) and procalcitonin (PCT), and evaluating whether their addition to severity scores (APACHE II and SOFA) could improve their prognostic accuracy. A single-centre prospective observational study conducted in an adult intensive care department at Marques de Valdecilla University Hospital in Spain. APACHE II and SOFA scores, CRP, PCT, suPAR and proADM levels on the day of ICU admission were collected. A total of 137 consecutive septic patients were studied. The best area under the curve (AUC) for the prediction of in-hospital mortality was for APACHE II (0.82) and SOFA (0.75) scores. The ROC curve for suPAR yielded an AUC of 0.67, higher than proADM (0.62), CRP (0.50) and PCT (0.44). Significant dose-response trends were found between hospital mortality and suPAR (OR Q4 = 4.83, 95% CI 1.60-14.62) and pro-ADM (OR Q4 = 3.00, 95% CI 1.06-8.46) quartiles. Non-significant associations were found for PCT and CRP. The combination of severity scores and each biomarker did not provide superior AUCs. SuPAR and, to a lesser extent, proADM levels on ICU admission were better tools in prognosticating in-hospital mortality than CRP or PCT. However, neither of the two new biomarkers has been demonstrated to be excessively useful in the current setting. The prognostic accuracy was better for severity scores than for any of the biomarkers.

  16. Corpos em Dobra: um ensaio gráfico-textual do Homeóstato #04 de José Alberto Marques

    Directory of Open Access Journals (Sweden)

    Luís Cláudio Costa Fajardo

    2016-09-01

    Full Text Available http://dx.doi.org/10.5007/1807-9288.2016v12n1p38 Este ensaio teórico-visual consiste em uma análise teórica e uma intervenção gráfica sobre o poema Homeóstato #04 da série Homeóstatos criada pelo poeta português José-Alberto Marques. A análise teórica surge de uma aproximação entre o poema e o conceito de Autopoiesis formulado por Humberto Maturana e Francisco Varela na década de 1970. A intervenção gráfica inspira-se na sugestão da forma poética desenhada pelo poeta digital brasileiro, André Vallias em seu poema “Nous n'avons pas compris Descartes” concebido em 1991.

  17. La escenificación del poder: el marqués de Castelldosrius, primer virrey Borbón del Perú (1707-1710

    Directory of Open Access Journals (Sweden)

    Sala i Vila, Núria

    2004-06-01

    Full Text Available The change in dynasty brought with it a series of transformations to Peruvian viceroyalty political culture. Analysis is made of the government of the Marquis of Castelldosrius (1707-1710 who, in his eagerness to increase a meagre patrimony, was to attenuate his conflicts with the Creole sectors and the Consulate, with a style of politics that was characterised by courtly and cultural uses that included traditions that were in vogue in the courts of Louis XIV and in his native Catalonia.

    El cambio dinástico comportó una serie de cambios en la cultura política virreinal peruana. Se analiza el gobierno del marqués de Castelldosrius (1707-1710, quien atenuaría sus conflictos con sectores criollos y del Consulado por su afán de acrecentar su magro patrimonio, con un estilo de ejercer la política, caracterizado por usos cortesanos y culturales que incorporaban tradiciones en boga en la corte de Luis XIV y en su Cataluña natal.

  18. Effet de l’endossement par les célébrités sur l’image de marque : Rôle médiateur de la congruence avec l’image de soi

    OpenAIRE

    Jaoued-Abassi, Leyla; Chandon, Jean-Louis

    2007-01-01

    International audience; This article studies the effect of celebrity endorsement on brand image. We examine how celebrity characteristics, in term of credibility and congruence with the brand, impact brand image. 942 respondents participated in this experiment. The results confirm the positive impact of celebrity credibility and congruence on brand image. The mediating role of self-congruity and the moderating role of brand familiarity and brand attitude are also examined.; Cette recherche an...

  19. Significance of cold defects in central skeleton on {sup 99}mTc-HMPAO labelled granulocyte scintigraphy; Scintigraphie aux polynucleaires marques par le {sup 99m}Tc-HMPAO: signification des hypofixations du squelette central

    Energy Technology Data Exchange (ETDEWEB)

    Couret, I.; Bertrand-Caix, J.; Cosculluela, S. [Hopital Pellegrin, 33 - Bordeaux (France); Auvert-Colin, L.; Fajon, O.; Rossi, M. [Hopital Lapeyronie, 34 - Montpellier (France)

    1994-12-31

    Couret et al address osteoarticular infections of the central skeleton which is a difficult condition to diagnose clinically. {sup 99m}Tc-hexamethylpropyleneamine oxime ({sup 99m}Tc-HMPAO) labelled granulocytes scintigraphy is of value for the detection of peripheral bone sepsis, but a cold defect is often encountered in central skeleton scans. The significance of a cold defect in 18 scintigraphic studies from patients referred with suspected infections of central skeleton is discussed. Of these 18 studies, only 4 cold defect corresponded to an acute or chronic sepsis and 6 to a healed sepsis. Of the remaining 8 studies, 4 corresponded to osteoarthritis, 1 to aggressive granulomatous lesions, 1 to chemical discitis, 1 to Paget`s disease, and 1 to bone metastase. All the cold defects on the labelled granulocytes scans corresponded to an increased uptake on the {sup 99m}Tc-diphosphonate ({sup 99m}Tc-MDP) scans. Among the cases studied, no increased uptake was found on the labelled granulocytes images; increased uptake of activity being required to classify the scintigraphy as positive in the appendicular skeleton. In fact, in central bone sepsis, the mechanism(s) inducing the destruction of the granulopoietic bone marrow or an obstacle to the granulocyte migration is not yet well established. We concluded that increased uptake of activity being very rare and cold defect having no specificity, labelled granulocytes scintigraphy is of lower interest in central skeleton bone sepsis detection than in peripheral bone sepsis detection. (authors). 51 refs., 4 figs., 1 tab.

  20. Labelled octreotide and relapse meningioma for a pre therapy evaluation by Sandrostatine: about one case; Octreotide marque et meningiome recidivant pour un bilan pretherapeuthique par sandostatine: a propos d'un cas

    Energy Technology Data Exchange (ETDEWEB)

    Viau, P.; Franken, P.; Benisvy, D.; Fontana, X.; Bussiere, F. [Centre Antoine-Lacassagne, Nice, (France); Darcourt, J. [CHU de Nice, (France)

    2009-05-15

    The scintigraphy with octreotide confirmed the wealth in somatostatin in the recurrent meningioma receptors and allowed to establish the treatment by somatostatin for our patient. Although, this examination does not have a prognosis contribution on the therapy efficiency, the proof of S.S.T.2 expression is a condition necessary to the treatment. (N.C.)

  1. PAR-1 and PAR-2 Expression Is Enhanced in Inflamed Odontoblast Cells.

    Science.gov (United States)

    Alvarez, M M P; Moura, G E; Machado, M F M; Viana, G M; de Souza Costa, C A; Tjäderhane, L; Nader, H B; Tersariol, I L S; Nascimento, F D

    2017-12-01

    Protease-activated receptors (PARs) are G protein-coupled receptors, which are activated by proteolytical cleavage of the amino-terminus and act as sensors for extracellular proteases. We hypothesized that PAR-1 and PAR-2 can be modulated by inflammatory stimulus in human dental pulp cells. PAR-1 and PAR-2 gene expression in human pulp tissue and MDPC-23 cells were analyzed by quantitative polymerase chain reaction. Monoclonal PAR-1 and PAR-2 antibodies were used to investigate the cellular expression of these receptors using Western blot, flow cytometry, and confocal microscopy in MDPC-23 cells. Immunofluorescence assays of human intact and carious teeth were performed to assess the presence of PAR-1 and PAR-2 in the dentin-pulp complex. The results show for the first time that human odontoblasts and MDPC-23 cells constitutively express PAR-1 and PAR-2. PAR-2 activation increased significantly the messenger RNA expression of matrix metalloproteinase (MMP)-2, MMP-9, MMP-13, and MMP-14 in MDPC-23 cells ( P < 0.05), while the expression of these enzymes decreased significantly in the PAR-1 agonist group ( P < 0.05). The high-performance liquid chromatography and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry analysis showed the presence of MMP-13 activity cleaving PAR-1 at specific, noncanonical site TLDPRS42↓F43LL in human dental pulp tissues. Also, we detected a presence of a trypsin-like activity cleaving PAR-2 at canonical site SKGR20↓S21LIGRL in pulp tissues. Confocal microscopy analysis of human dentin-pulp complex showed intense positive staining of PAR-1 and PAR-2 in the odontoblast processes in dentinal tubules of carious teeth compared to intact ones. The present results support the hypothesis of activation of the upregulated PAR-1 and PAR-2 by endogenous proteases abundant during the inflammatory response in dentin-pulp complex.

  2. Differences in clinical characteristics of fallopian canal dehiscence associated with pars flaccida and pars tensa cholesteatomas.

    Science.gov (United States)

    Shinnabe, Akihiro; Yamamoto, Hiroki; Hara, Mariko; Hasegawa, Masayo; Matsuzawa, Shingo; Kanazawa, Hiromi; Yoshida, Naohiro; Iino, Yukiko

    2014-08-01

    This study investigated the difference in clinical characteristics of fallopian canal dehiscence associated with pars flaccida and pars tensa cholesteatomas for the purpose of increasing the preoperative detectability of dehiscence. A total of 189 ears of patients 7-80 years of age (mean 42 years) with pars flaccida cholesteatoma and 63 ears of patients 9-84 years of age (mean 50 years) with pars tensa cholesteatoma were studied. All patients had undergone prior surgical management at our institution from January 2006 to April 2012. The incidence of fallopian canal dehiscence and its location were compared between pars flaccida and pars tensa cholesteatomas. Intraoperative findings of coexistent pathologies, including destruction of the stapes superstructure, labyrinthine fistula, and dural exposure, were compared between the dehiscence and no-dehiscence groups for the two types of cholesteatomas. The incidence of dehiscence was significantly higher in patients with pars tensa cholesteatoma (55.6 %) than in patients with pars flaccida cholesteatoma (26.5 %). Dehiscence located posterior to the cochleariform process occurred slightly more frequently in patients with pars tensa cholesteatoma than in those with pars flaccida cholesteatoma. In patients with pars flaccida cholesteatoma, labyrinthine fistulas and dural exposure were significantly more frequent in the dehiscence group than in the no-dehiscence group. Fallopian canal dehiscence is more frequent in patients with pars tensa cholesteatoma than in those with pars flaccida cholesteatoma. Especially in patients with pars flaccida cholesteatoma, paying special attention to these coexisting pathologies is important to increase preoperative detectability of dehiscence.

  3. La industria lítica del yacimiento achelense de Torralba (Soria, España. Colecciones marqués de Cerralbo y Howell

    Directory of Open Access Journals (Sweden)

    Sánchez-Cervera, Borja

    2015-06-01

    Full Text Available Torralba is a Middle Pleistocene site, with absolute dates that place it in MIS 7. Its lithic industry comes from fluvial levels and is situated in a secondary position. The raw materials were brought to the site from sources located several tens kilometers away. The techno-economic study of the industrial series obtained in the excavations conducted by Marquis of Cerralbo (1909-1913 and Clark Howell (1961-1963, confirms the Acheulean identity of this industry. Production, configuration and maintenance of the industry were all carried out at the site, although at least some macro-tools were introduced already elaborated. We have identified unusual technological aspects in the context of the European Acheulean, for example the frequent use of flint in developing cleavers.Torralba es un yacimiento del Pleistoceno medio, con dataciones numéricas que le sitúan en el MIS 7 (191/243 ka. Su industria, que procede de niveles fluviales y se encuentra fundamentalmente en posición secundaria, fue elaborada sobre materias primas introducidas desde distancias que pueden alcanzar varias decenas de kilómetros. El estudio tecno-económico de las series industriales obtenidas en las excavaciones realizadas por el marqués de Cerralbo (1909-1913 y Clark Howell (1961-1963 confirma la identidad achelense de la industria y la realización de procesos de producción, configuración y mantenimiento en el yacimiento, aunque al menos una parte del macroutillaje fue introducida ya configurada en el mismo. Se identifican aspectos tecnológicos singulares en el contexto del achelense europeo, como el uso frecuente del sílex en la elaboración de hendedores.

  4. Hummingbird Ecstasy footprint and cultural guide for vine and wine. The case of "Marqués of Villa de Leyva"

    Directory of Open Access Journals (Sweden)

    Fabio Aldemar Gómez Sierra

    2012-10-01

    Full Text Available In this article reflecting on viticulture in Boyacá, is analyzed from the project and its product Ain Karim wine Marqués de Villa de Leyva, located in the Valley of Zaquencipá, a structured system of meanings that this type of agriculture does unite in place where it is installed. Historically, viticultural practices have managed to involve substantial elements of cultural dynamics: belief systems, identity, health and disease, and ways to socialize economy ingesting fermented beverages. The wine "Marquis of Villa de Leyva" emerges in a context of climate, soil and subsoil, with potential for development characteristics, which however must be certified with specialized studies of tropical modern winemaking. In this Zaquencipá Valley, as it was called ancestrally, the religious geosmbólica was irradiated by Iguaquemountains, sacred space Muiscas. The conquest and colonization transformed it by the Christian geosimbólica, economic (production of wheat and military. Currently the town of Villa de Leyva and his environment is attractive to tourists, middle-class residents and significant capital, in this context arises the Vineyard founded in the early eighties by Mr. Pablo Toro, originally imported strains Cabernet Sauvignon, Chardonnay and Sauvignon Blanc. This replicates the culture of the vine and wine, which is also supported by a constant of aesthetics, hygiene and ethno-specific technical. its process leaves substantial perceive elements of cultural globalization, internationalization and localities need to analyze the ethno-regional production techniques.

  5. SUIVI DE LA MIGRATION D’AVALAISON DES ANGUILLES PAR UNE CONDUITE DE DEBIT RESERVE

    Directory of Open Access Journals (Sweden)

    LEGAULT A.

    2003-07-01

    Full Text Available Le suivi de la migration catadrome de l’anguille par une conduite de débit réservé a été réalisé sur le barrage de la retenue de Bois Joli sur le Frémur (dénivelé : 13,7 m ; 3 millions de m3. Une partie des migrateurs utilise cette conduite, au début de la saison de dévalaison quand elle représente la seule voie de transit mais également plus tard, lorsque le barrage déverse et que s’ouvre ainsi une seconde voie de passage. En raison des faibles débits d’alimentation et de l’implantation de l’entrée de la conduite on peut supposer que les anguilles prospectent la retenue pour trouver une voie de transit. L’efficacité de l’équipement, qui s’établit à 12 % de la dévalaison totale, concourt donc pour une part non négligeable au transit des anguilles. La partition des débits entre les différentes voies de migration semblerait essentielle pour définir leur efficacité. Malgré l’aménagement de la conduite, on observe une mortalité de 11 % des anguilles qui l’empruntent. Elle semble essentiellement liée à l’abrasion contre les parois et le papillon de la vanne. Dans de nombreux cas, la mortalité lors du passage dans les conduites de débit réservé doit être très importante, voire totale en raison de leur configuration et de leur mode de gestion. Cette mortalité tend à réduire le nombre des géniteurs produit par le bassin versant. Les anguilles marquées qui ont franchi le barrage grâce à la conduite avant le pic de migration stoppent leur progression en aval. Elles la reprennent quelques semaines plus tard quand le barrage déverse ou l’année suivante. Ce second constat confirme les effets des retenues sur la vitesse de migration. L’utilisation des conduites de débit réservé par les anguilles d’avalaison devrait être prise en compte lors de leur conception et de leur gestion afin de réduire les risques de mortalité.

  6. Ouvrages d’assainissement des eaux et qualité du milieu récepteur en zone urbaine : cas de rejets dans La Marque à Villeneuve d’Ascq

    OpenAIRE

    Ivanovsky, Anastasia

    2016-01-01

    The Marque River is a little watercourse located in a peri-urban watershed in Northern France, going through agricultural and urban areas. Nearby Villeneuve d’Ascq, this river receives waters from two main water treatment plants: (i) the waste water treatment plant of Villeneuve d’Ascq; and (ii) the waters from a stormwater basin, the Heron lake. In order to improve the understanding of the functioning of the river in this area, low and high frequencies monitoring have been undertaken in 2014...

  7. The ParB-parS Chromosome Segregation System Modulates Competence Development in Streptococcus pneumoniae.

    Science.gov (United States)

    Attaiech, Laetitia; Minnen, Anita; Kjos, Morten; Gruber, Stephan; Veening, Jan-Willem

    2015-06-30

    ParB proteins bind centromere-like DNA sequences called parS sites and are involved in plasmid and chromosome segregation in bacteria. We previously showed that the opportunistic human pathogen Streptococcus pneumoniae contains four parS sequences located close to the origin of replication which are bound by ParB. Using chromatin immunoprecipitation (ChIP), we found here that ParB spreads out from one of these parS sites, parS(-1.6°), for more than 5 kb and occupies the nearby comCDE operon, which drives competence development. Competence allows S. pneumoniae to take up DNA from its environment, thereby mediating horizontal gene transfer, and is also employed as a general stress response. Mutating parS(-1.6°) or deleting parB resulted in transcriptional up-regulation of comCDE and ssbB (a gene belonging to the competence regulon), demonstrating that ParB acts as a repressor of competence. However, genome-wide transcription analysis showed that ParB is not a global transcriptional regulator. Different factors, such as the composition of the growth medium and antibiotic-induced stress, can trigger the sensitive switch driving competence. This work shows that the ParB-parS chromosome segregation machinery also influences this developmental process. Streptococcus pneumoniae (pneumococcus) is an important human pathogen responsible for more than a million deaths each year. Like all other organisms, S. pneumoniae must be able to segregate its chromosomes properly. Not only is understanding the molecular mechanisms underlying chromosome segregation in S. pneumoniae therefore of fundamental importance, but also, this knowledge might offer new leads for ways to target this pathogen. Here, we identified a link between the pneumococcal chromosome segregation system and the competence-developmental system. Competence allows S. pneumoniae to take up and integrate exogenous DNA in its chromosome. This process plays a crucial role in successful adaptation to--and escape from

  8. ParAB Partition Dynamics in Firmicutes: Nucleoid Bound ParA Captures and Tethers ParB-Plasmid Complexes.

    Directory of Open Access Journals (Sweden)

    Virginia S Lioy

    Full Text Available In Firmicutes, small homodimeric ParA-like (δ2 and ParB-like (ω2 proteins, in concert with cis-acting plasmid-borne parS and the host chromosome, secure stable plasmid inheritance in a growing bacterial population. This study shows that (ω:YFP2 binding to parS facilitates plasmid clustering in the cytosol. (δ:GFP2 requires ATP binding but not hydrolysis to localize onto the cell's nucleoid as a fluorescent cloud. The interaction of (δ:CFP2 or δ2 bound to the nucleoid with (ω:YFP2 foci facilitates plasmid capture, from a very broad distribution, towards the nucleoid and plasmid pairing. parS-bound ω2 promotes redistribution of (δ:GFP2, leading to the dynamic release of (δ:GFP2 from the nucleoid, in a process favored by ATP hydrolysis and protein-protein interaction. (δD60A:GFP2, which binds but cannot hydrolyze ATP, also forms unstable complexes on the nucleoid. In the presence of ω2, (δD60A:GFP2 accumulates foci or patched structures on the nucleoid. We propose that (δ:GFP2 binding to different nucleoid regions and to ω2-parS might generate (δ:GFP2 gradients that could direct plasmid movement. The iterative pairing and unpairing cycles may tether plasmids equidistantly on the nucleoid to ensure faithful plasmid segregation by a mechanism compatible with the diffusion-ratchet mechanism as proposed from in vitro reconstituted systems.

  9. ¡París!

    Directory of Open Access Journals (Sweden)

    Beatriz Caballero

    2003-01-01

    Full Text Available El día no le alcanzaba para caminarlo. Conocía cada puente, cada calle,plaza, museo, rincón, iglesia, café, y la mesa con el mejor ángulo para mirar siempre una iglesia, ojalá Saint-Germain-des-Pres, o Notre-Dame. Siempre contaba la impresión que le había causado a Bolívar ver allí cuando el papa coronó a Napoleón. Él se iba para la oficina todos los días, Luis a un taller a pintar, Antonio a estudiar ciencias políticas, yo al colegio y mamá hacía las cosas prácticas. Aprendíamos francés todos al tiempo y por la noche nos contábamos lo nuevo que cada uno había aprendido. Mamá después, cuando ya se quería volver, decía que París nos había maleado a todos. Estaba en pleno furor el cine francés de la "nueva ola", pero ella sólo me llevaba a ver películas de vaqueros pues no se fiaba de la clasificación de los periódicos.

  10. O srpskim prevodnim ekvivalentima predloga par u romanu Francusko zaveštanje Andreja Makina

    Directory of Open Access Journals (Sweden)

    Stanković Selena

    2007-01-01

    Full Text Available (francuski Dans la langue française d'aujourd'hui, les prépositions marquent avec précision de délicats rapports entre les mots. Par, une des prépositions les plus fréquentes, indique de nombreux rapports entre les mots et les syntagmes et c'est le fait que les équivalents serbes illustrent clairement. Ainsi l'analyse montre que les équivalents de traduction de par dans le serbe sont les prépositions différentes dans les constructions du type préposition cas, en indiquant en même temps de divers rapports casuels: od (le génitif au sens de cause; za (l'accusatif et la valeur de direction, de destination et de temps et l'instrumental en fonction du complément circonstanciel de manière; u (l'accusatif et la fonction du complément circonstanciel de temps et le locatif qui marque la manière; kroz (l'accusatif et la valeur de lieu, d'espace; po (le locatif au sens de moyen; zbog (le génitif et la valeur de cause; na (l'accusatif qui indique le lieu après le mouvement, la quantité du temps et la quantité en général; preko (le génitif au sens de moyen et de lieu au-dessus de quelque chose; iz et usled (le génitif marquant la cause; uz (l'accusatif et la fonction du complément circonstanciel de manière et de moyen; sa (le sociatif et l'instrumental de moyen; oko (le génitif et la valeur d'espace; pod (l'instrumental indiquant le lieu au-dessous de quelque chose. Quelquefois, le syntagme prépositionnel obtient pour l'équivalent de traduction un nom ou un syntagme nominal au cas sans préposition; les cas exprimés sont: l'instrumental (indiquant le moyen l'agent, la cause et la manière et le génitif (temporel et partitif. Les phrases passives et parfois les constructions avec le syntagme prépositionnel ayant la fonction du complément de l'adjectif sont traduites par des phrases actives. De nombreuses locutions construites avec par (locutions prépositionnelles, locutions adverbiales, etc ont pour les équivalents serbes

  11. C9.A/14 steelwork joints de poutres par plaque frontale : assemblages par gousset

    CERN Document Server

    2015-01-01

    Les Tables de résistances ultimes des assemblages boulonnés par plaque frontale et par gousset, complétées par une description des modèles de calcul et des exemples d’application, ont pour but de faciliter la tâche de l'ingénieur et du constructeur. Cette première partie C9.A/14 contient les chapitres suivants: - Joints de poutres par plaque frontale en acier S235 et S355 - Assemblages par gousset en acier S235 et S355 Les Tables contiennent des données relatives à la géométrie ainsi que les valeurs de calcul correspondantes des résistances ultimes des assemblages ; elles remplacent le chapitre « Assemblages par plaques frontales et boulons HR » des anciennes Tables C9.1 de 1983 / 2002. Le calcul de ces assemblages par plaque frontale est basé sur les hypothèses du modèle de la méthode des composants décrite dans la norme SN EN 1993-1-8. Les vérifications sont effectuées selon la norme SIA 263:2013. Les assemblages par gousset remplacent les assemblages par double cornière, (telle...

  12. Structural analysis of the ParR/parC plasmid partition complex

    DEFF Research Database (Denmark)

    Møller-Jensen, Jakob; Ringgaard, Simon; Mercogliano, Christopher P

    2007-01-01

    . coli plasmid pB171. ParR forms a tight dimer resembling a large family of dimeric ribbon-helix-helix (RHH)2 site-specific DNA-binding proteins. Crystallographic and electron microscopic data further indicate that ParR dimers assemble into a helix structure with DNA-binding sites facing outward. Genetic...

  13. Protease-activated receptor (PAR)-2 is required for PAR-1 signalling in pulmonary fibrosis

    NARCIS (Netherlands)

    Lin, Cong; von der Thüsen, Jan; Daalhuisen, Joost; ten Brink, Marieke; Crestani, Bruno; van der Poll, Tom; Borensztajn, Keren; Spek, C. Arnold

    2015-01-01

    Idiopathic pulmonary fibrosis is the most devastating diffuse fibrosing lung disease of unknown aetiology. Compelling evidence suggests that both protease-activated receptor (PAR)-1 and PAR-2 participate in the development of pulmonary fibrosis. Previous studies have shown that bleomycin-induced

  14. LES LESIONS DES ORGANES GENITAUX EXTERNES PAR ARME A

    African Journals Online (AJOL)

    Les lésions par arme à feu définies comme toute blessure produite sur le corps humain par rapprochement ou le choc d'une arme à feu, sont généralement rares qu'il s'agisse de plaies par armes de guerre ou de plaies par projectiles dans un contexte civil“. Parmi les facteurs de gravité des plaies par balles figu-.

  15. Traitement des eaux par électrofloculation

    OpenAIRE

    Cansado, Isabel

    1997-01-01

    Traitement des eaux par electrofloculation Les écosystèmes aquatiques sont très affectés par les eaux de rejet qui sont déversées dans les cours d’eau. La charge de ces eaux existe sous diverses formes: solutés, colloïdes ou particules. Il est important de traiter l’eau usée à de faibles coûts au lieu de la rejeter directement dans le milieu récepeteur. Dans le but d’améliorer les procédés physico-chimiques de déstabilisation des émulsions, nous étudions les traitements par électrocoagulat...

  16. Anníbal marques da costa e a "matemática em versos e prosas": histórias da matemática na são joão del-rei do início do século XX Anníbal Marques da Costa and the "Matemática em versos e prosas": histories of mathematics in Sao João del-Rei in the early twentieth century

    Directory of Open Access Journals (Sweden)

    Romélia Mara Alves Souto

    2011-01-01

    Full Text Available Neste artigo, apresentamos os resultados de um estudo analítico-descritivo que realizamos da obra "Matemática em versos e prosas", de Anníbal Marques da Costa, encontrada no acervo do Clube Teatral Artur Azevedo, na Universidade Federal de São João del-Rei (UFSJ, em Minas Gerais. A obra é um manuscrito, em dois volumes, produzida entre 1942 e 1954, que, com explícita intenção de divulgação da matemática, embora nunca tenha sido impressa, trata de conceitos e propriedades da matemática elementar. A investigação possibilitou-nos elaborar uma descrição seguida de uma análise histórica e crítica do texto, que foi enriquecida pelo acréscimo de algumas informações sobre o autor e a época em que ele viveu.This paper presents the results of an analytical-descriptive study of the book "Matemática em versos e prosas" by Anníbal Marques da Costa, found in Clube Teatral Artur Azevedo, archives from the Universidade Federal de São João del-Rei (UFSJ, in Minas Gerais. The book has never been published and is a manuscript in two volumes produced between 1942 and 1954 aiming to promote Mathematics. It deals with the concepts and properties of elementary mathematics. The research enabled the development of a description and a historical and critical analysis of the text, enriched by some information about the author and the time he lived.

  17. El retrato del obispo Jan Malderus de Van Dyck identificado en la antigua colección del Marqués del Carpió

    Directory of Open Access Journals (Sweden)

    Díaz Padrón, Matías

    2005-12-01

    Full Text Available No es fácil exponer el estado de la cuestión en cuanto a los retratos conocidos del obispo Jan Malderus, con diferente grado de calidad y copiosa documentación literaria. Los últimos estudios reclaman la existencia de un original perdido, al tratar de la repetición de taller de la antigua colección de Pierre Crozat en París [Lienzo, 115 x 96 cm]. Hoy pienso que se trata de la tabla que se encuentra en la actualidad en la Fundación BSCH, desconocida en los estudios consagrados al pintor [125 x 97 cm. Fig. 1]. Los grabados de Johannes Meysens [Fig. 3] y Adrianus Lommelin [Fig. 4] " testifican la identidad del personaje, y el encargo a Van Dyck por parte del omnipotente obispo. El último no está invertido respecto a la pintura como sería habitual, pero es coherente con la tabla que estudiamos y con las repeticiones que tratamos a continuación…

  18. A 66-kDa protein of bovine hypophyseal Pars tuberalis induces luteinizing hormone release from rat Pars distalis.

    Science.gov (United States)

    Lafarque, Martha; Oliveros, Liliana

    2008-12-01

    In this study, evidence for a factor secreted by bovine hypophyseal pars tuberalis that stimulates luteinizing hormone (LH) release from rat pars distalis cells is shown. The secretion products of bovine pars tuberalis cells into the culture medium were assayed on dispersed rat pars distalis cells in 30 min incubations and superfusion experiments. The culture medium from pars tuberalis total cell populations, added at a dose of 6 microg per tube, induced the greater LH release from pars distalis cells, without effect on follicle stimulating hormone (FSH) release. After pars tuberalis cells separation on a discontinuos Percoll gradient, only the culture medium of cells from 50 and 60% strength Percoll were able to release LH from rat pars distalis cells. Therefore, cell fractions from 50 and 60% strenght Percoll were cultured together. To elicit maximal LH release (6 times the basal output), with the addition of 2 microg of pars tuberalis protein was required, suggesting that these cells produce the factor or factors which affect pars distalis gonadotrope cells. After applying the pars tuberalis culture medium on 12% SDS-PAGE, the band with biological activity was that of 66-kDal. Fifty ng protein of its eluate released almost 9 times the basal output of LH from pars distalis cells. Results suggest a modulating effect of a protein from the bovine pars tuberalis on rat cultured gonadotrope cells from the pars distalis.

  19. Les effets du travail en equipe dans l'apprentissage par projets sur la motivation des etudiantes et des etudiants en formation des ingenieurs

    Science.gov (United States)

    Fernandez, Nicolas

    Les representants des secteurs industriels et les, milieux professionnels en Amerique du Nord reprochaient aux universites de former des ingenieurs avec peu d'experience pratique en resolution de problemes et en conception. Quelques programmes de genie ont alors mis en place le travail en equipe dans l'apprentissage par projets. Beaucoup d'ecrits font valoir les benefices de l'apprentissage par projets sur la motivation des etudiants. Or, ces benefices commencent a peine a faire l'objet de recherches visant a produire des donnees probantes a ce sujet. Les travaux sur la motivation en contexte d'apprentissage et les modeles theoriques developpes sont issus d'environnements d'apprentissage marques par l'enseignement magistral. Le modele de la valeur attendue de la tache (Eccles et Wigfield, 1995; Neuville, 2004) et le modele du systeme-groupe (St-Arnaud, 2008) ont ete retenus pour mesurer les effets du travail en equipe dans l'apprentissage par projets sur la motivation. La recherche visait aussi a approfondir et a nuancer la comprehension de la motivation des etudiants universitaires apprenant en contexte innovant. Les sujets constituent des etudiants (n=100) travaillant sur des projets d'integration au cours des trois sessions initiales du programme de genie mecanique d'une universite canadienne. L'analyse de regression multiple revele que les construits de la motivation expliquent un tiers de la variance de l'engagement academique dans la realisation du projet d'integration. Les perceptions de l' "expectancy", de la valeur intrinseque et utilitaire sont les determinants principaux de l'engagement des etudiants. L'analyse de variance multivariee a mesures repetees indique que la motivation des etudiants pour le travail sur les projets d'integration a augmente au cours des trois sessions initiales du parcours de formation. Finalement, malgre l'absence d'interaction significative entre les variables de motivation et de l'equipe, les reponses des sujets indiquent une

  20. Par-4: A New Activator of Myosin Phosphatase

    Science.gov (United States)

    Vetterkind, Susanne; Lee, Eunhee; Sundberg, Eric; Poythress, Ransom H.; Tao, Terence C.; Preuss, Ute

    2010-01-01

    Myosin phosphatase (MP) is a key regulator of myosin light chain (LC20) phosphorylation, a process essential for motility, apoptosis, and smooth muscle contractility. Although MP inhibition is well studied, little is known about MP activation. We have recently demonstrated that prostate apoptosis response (Par)-4 modulates vascular smooth muscle contractility. Here, we test the hypothesis that Par-4 regulates MP activity directly. We show, by proximity ligation assays, surface plasmon resonance and coimmunoprecipitation, that Par-4 interacts with the targeting subunit of MP, MYPT1. Binding is mediated by the leucine zippers of MYPT1 and Par-4 and reduced by Par-4 phosphorylation. Overexpression of Par-4 leads to increased phosphatase activity of immunoprecipitated MP, whereas small interfering RNA knockdown of endogenous Par-4 significantly decreases MP activity and increases MYPT1 phosphorylation. LC20 phosphorylation assays demonstrate that overexpression of Par-4 reduces LC20 phosphorylation. In contrast, a phosphorylation site mutant, but not wild-type Par-4, interferes with zipper-interacting protein kinase (ZIPK)-mediated MP inhibition. We conclude from our results Par-4 operates through a “padlock” model in which binding of Par-4 to MYPT1 activates MP by blocking access to the inhibitory phosphorylation sites, and inhibitory phosphorylation of MYPT1 by ZIPK requires “unlocking” of Par-4 by phosphorylation and displacement of Par-4 from the MP complex. PMID:20130087

  1. CFD analysis of PAR performance as function of inlet design

    Energy Technology Data Exchange (ETDEWEB)

    Park, Kweonha, E-mail: khpark@kmou.ac.kr [Division of Mechanical and Energy systems Engineering, Korea Maritime University, Dongsam-dong, Yeongdo-gu, Busan 606-791 (Korea, Republic of); Khor, Chong Lee, E-mail: itachi_829@hotmail.com [Department of Mechanical Engineering, Korea Maritime University (Korea, Republic of)

    2016-01-15

    Highlights: • The new concept of PAR (passive autocatalytic recombiner) was proposed and analyzed. • Guidance wall was added at the bottom of PAR to enhance the flow rate through the catalyst. • The new concept of PAR was proved to have a better hydrogen removal performance. - Abstract: Passive autocatalytic recombiner (PAR) is very useful hydrogen mitigation measurement. It is widely implemented in the current and advanced light water reactors (ALWRs). The design of the PARs should be optimized for the specific use under severe accident scenarios. Several techniques and innovations have been fused into the PAR, as an effort to increase its efficiency of hydrogen mitigation. This study proposes different concepts of PAR, which applied some changes to the honeycomb catalyst PAR made by the Korea Nuclear Technology (KNT) Inc. Two slices of plate are added to the bottom of PAR model, which intended to act as a reflection wall and promote the gas flow into PAR. Hydrogen volume fraction was given 4 vol. % which tested by KNT to investigate the performance of PAR in different direction gas flow conditions to see maximum hydrogen recombination rate. The new concept of PAR was proved to have a better hydrogen removal performance compared to the original honeycomb catalyst PAR.

  2. PAR-TERRA directs homologous sex chromosome pairing.

    Science.gov (United States)

    Chu, Hsueh-Ping; Froberg, John E; Kesner, Barry; Oh, Hyun Jung; Ji, Fei; Sadreyev, Ruslan; Pinter, Stefan F; Lee, Jeannie T

    2017-08-01

    In mammals, homologous chromosomes rarely pair outside meiosis. One exception is the X chromosome, which transiently pairs during X-chromosome inactivation (XCI). How two chromosomes find each other in 3D space is not known. Here, we reveal a required interaction between the X-inactivation center (Xic) and the telomere in mouse embryonic stem (ES) cells. The subtelomeric, pseudoautosomal regions (PARs) of the two sex chromosomes (X and Y) also undergo pairing in both female and male cells. PARs transcribe a class of telomeric RNA, dubbed PAR-TERRA, which accounts for a vast majority of all TERRA transcripts. PAR-TERRA binds throughout the genome, including to the PAR and Xic. During X-chromosome pairing, PAR-TERRA anchors the Xic to the PAR, creating a 'tetrad' of pairwise homologous interactions (Xic-Xic, PAR-PAR, and Xic-PAR). Xic pairing occurs within the tetrad. Depleting PAR-TERRA abrogates pairing and blocks initiation of XCI, whereas autosomal PAR-TERRA induces ectopic pairing. We propose a 'constrained diffusion model' in which PAR-TERRA creates an interaction hub to guide Xic homology searching during XCI.

  3. Centromere pairing by a plasmid-encoded type I ParB protein

    DEFF Research Database (Denmark)

    Ringgaard, Simon; Löwe, Jan; Gerdes, Kenn

    2007-01-01

    The par2 locus of Escherichia coli plasmid pB171 encodes two trans-acting proteins, ParA and ParB, and two cis-acting sites, parC1 and parC2, to which ParB binds cooperatively. ParA is related to MinD and oscillates in helical structures and thereby positions ParB/parC-carrying plasmids regularly...

  4. SAFARI 2000 PAR Measurements, Kalahari Transect, Botswana, Wet Season 2000

    Data.gov (United States)

    National Aeronautics and Space Administration — ABSTRACT: Ceptometer data from a Decagon AccuPAR (Model PAR-80) were collected at four sites in Botswana during the SAFARI 2000 Kalahari Transect Wet Season Campaign...

  5. SAFARI 2000 PAR Measurements, Kalahari Transect, Botswana, Wet Season 2000

    Data.gov (United States)

    National Aeronautics and Space Administration — Ceptometer data from a Decagon AccuPAR (Model PAR-80) were collected at four sites in Botswana during the SAFARI 2000 Kalahari Transect Wet Season Campaign (March,...

  6. A Combined Global and Local Approach to Elucidate Spatial Organization of the Mycobacterial ParB-parS Partition

    Energy Technology Data Exchange (ETDEWEB)

    Chaudhuri, Barnali [University of Buffalo, The State University of New York; Gupta, Sayan [Case Western Reserve University; Urban, Volker S [ORNL; Chance, Mark [Case Western Reserve University; D' Mello, Rhijuta [Case Western Reserve University; Smith, Lauren [University of Buffalo, The State University of New York; Lyons, Kelly [University of Buffalo, The State University of New York; Gee, Jessica [University of Buffalo, The State University of New York

    2010-01-01

    Combining diverse sets of data at global (size, shape) and local (residue) scales is an emerging trend for elucidating the organization and function of the cellular assemblies. We used such a strategy, combining data from X-ray and neutron scattering with H/D-contrast variation and X-ray footprinting with mass spectrometry, to elucidate the spatial organization of the ParB-parS assembly from Mycobacterium tuberculosis. The ParB-parS participates in plasmid and chromosome segregation and condensation in predivisional bacterial cells. ParB polymerizes around the parS centromere(s) to form a higher-order assembly that serves to recruit cyto-skeletal ParA ATPases and SMC proteins for chromosome segregation. A hybrid model of the ParB-parS was built by combining and correlating computational models with experiment-derived information about size, shape, position of the symmetry axis within the shape, internal topology, DNA-protein interface, exposed surface patches, and prior knowledge. This first view of the ParB-parS leads us to propose how ParB spread on the chromosome to form a larger assembly.

  7. A Combined Global and Local Approach to Elucidate Spatial Organization of the Mycobacterial ParB-parS Partition Assembly

    Energy Technology Data Exchange (ETDEWEB)

    B Chaudhuri; S Gupta; V Urban; M Chance; R DMello; L Smith; K Lyons; J Gee

    2011-12-31

    Combining diverse sets of data at global (size, shape) and local (residue) scales is an emerging trend for elucidating the organization and function of the cellular assemblies. We used such a strategy, combining data from X-ray and neutron scattering with H/D-contrast variation and X-ray footprinting with mass spectrometry, to elucidate the spatial organization of the ParB-parS assembly from Mycobacterium tuberculosis. The ParB-parS participates in plasmid and chromosome segregation and condensation in predivisional bacterial cells. ParB polymerizes around the parS centromere(s) to form a higher-order assembly that serves to recruit cyto-skeletal ParA ATPases and SMC proteins for chromosome segregation. A hybrid model of the ParB-parS was built by combining and correlating computational models with experiment-derived information about size, shape, position of the symmetry axis within the shape, internal topology, DNA-protein interface, exposed surface patches, and prior knowledge. This first view of the ParB-parS leads us to propose how ParB spread on the chromosome to form a larger assembly.

  8. Host response biomarker in sepsis: suPAR detection.

    Science.gov (United States)

    Giamarellos-Bourboulis, Evangelos J; Georgitsi, Marianna

    2015-01-01

    Recent studies of our group have shown that suPAR may complement APACHE II score for risk assessment in sepsis. suPAR may be measured in serum of patients by an enzyme immunosorbent assay developed by Virogates (suPARnostic™). Production of suPAR from circulating neutrophils and monocytes may be assessed after isolation of neutrophils and monocytes and ex vivo culture. This is followed by measurement of suPAR in culture supernatants.

  9. Copenhagen uPAR prostate cancer (CuPCa) database

    DEFF Research Database (Denmark)

    Lippert, Solvej; Berg, Kasper D; Høyer-Hansen, Gunilla

    2016-01-01

    AIM: Urokinase plasminogen activator receptor (uPAR) plays a central role during cancer invasion by facilitating pericellular proteolysis. We initiated the prospective 'Copenhagen uPAR Prostate Cancer' study to investigate the significance of uPAR levels in prostate cancer (PCa) patients. METHODS...

  10. Les Brulures Chimiques Par Le Laurier Rose

    Science.gov (United States)

    Bakkali, H.; Ababou, M.; Nassim Sabah, T.; Moussaoui, A.; Ennouhi, A.; Fouadi, F.Z.; Siah, S.; Ihrai, H.

    2010-01-01

    Summary Le laurier rose ou Nerium oleander est un arbuste qui pousse naturellement dans les régions méditerranéennes. Au Maroc on le trouve dans les lieux humides. Il est réputé par ses risques de toxicité systémique en cas d'empoisonnement à cause de la présence de deux alcaloïdes, surtout l'oléandrine. La littérature illustre des cas d'utilisation locale des feuilles de cette plante contre la gale, les hémorroïdes et les furoncles. Nous rapportons deux cas de brûlures chimiques par le laurier rose de gravité différente. Cela doit aboutir à une information élargie de la population, ainsi qu'une réglementation stricte de sa commercialisation. PMID:21991211

  11. Les Brulures Chimiques Par Le Laurier Rose

    OpenAIRE

    Bakkali, H.; Ababou, M.; Nassim Sabah, T.; Moussaoui, A.; Ennouhi, A.; Fouadi, F.Z.; Siah, S.; Ihrai, H.

    2010-01-01

    Le laurier rose ou Nerium oleander est un arbuste qui pousse naturellement dans les régions méditerranéennes. Au Maroc on le trouve dans les lieux humides. Il est réputé par ses risques de toxicité systémique en cas d'empoisonnement à cause de la présence de deux alcaloïdes, surtout l'oléandrine. La littérature illustre des cas d'utilisation locale des feuilles de cette plante contre la gale, les hémorroïdes et les furoncles. Nous rapportons deux cas de brûlures chimiques par le laurier rose ...

  12. Deltagerinvolveret organisationsudvikling med PAR som metode

    DEFF Research Database (Denmark)

    Sparre, Mogens

    2017-01-01

    resistant to change. The quite widespread narrative of resistance to change, is believed to originate from the many top-down driven change projects implemented by the top management or/and external consultants. Is it possible to create a desired change in the form of the inter-subjective perception building......When a leader wants to initiate or influence some necessary changes in the organization, he must be aware of the fact that the subjects that form the organization create their own meaning about actions the manager wants implemented. This article confronts the assumption that people are by nature...... about the organization in an management group with a Participatory Action Research (PAR) case study? The article deals with a Phenomenological case study, where the researcher, with a PAR approach method, creates a research design with the purpose to influence the participant’s “Subjective perception...

  13. [Ambroise Paré in French literature].

    Science.gov (United States)

    Dumaitre, P

    1995-01-01

    The 16th century by its passionate side has been the favourite one of authors of historical novels in which among the heroes of "cloak and dagger stories" appears sometime Ambroise Paré. Alexandre Dumas (the father) has shown him at the court of Charles IX in La Reine Margot (1845) where he does not however play a great role. On the contrary, Balzac in Le Martyr calviniste (1842) has given him a capital part close to the dying François II, whom he intended to trepanize but had to give up this idea as a consequence of the opposition of the queen-mother Catherine de Médicis. In the present century, Robert Merle in Paris ma bonne ville (Fortune de France, 3, 1980) shows Paré at the time of the Saint Barthélemy.

  14. Los post-incunables de Cisneros de la Biblioteca Histórica Marqués de Valdecilla de la Complutense y la música de Antonio de Cabezón

    Directory of Open Access Journals (Sweden)

    Burgos Bordonau, Esther

    2011-01-01

    Full Text Available The following article talks about several post-incunabula that belonged to the period of Cardenal Cisneros, and are nowadays kept in the Biblioteca Histórica Marqués de Valdecilla (UCM. These books are well known not only in the history of printing but also in the history of music and liturgy due to several reasons. The study has been practised in five different books (Psalterium, 1515; 2 copies of the Intonarium Toletanum, 1519, 1520; and 2 copies of the Diurnum 1519,1520 that have been bibliographically described as well as their bindings and also their musical aspects. It's remarkable to point out that these melodies were all well known at Antonio de Cabezón's time, famous Spanish composer and organist, who could have inspired himself in some of these melodies for his further compositions = El artículo que a continuación se presenta da noticia de una serie de post-incunables de la época del Cardenal Cisneros que actualmente se custodian en la Biblioteca Histórica Marqués de Valdecilla (UCM. Dichos libros han pasado a la historia de la imprenta así como también a la historia de la música y de la liturgia por múltiples motivos. El estudio se ha realizado sobre cinco ejemplares (Psalterium, 1515; 2 ejemplares del Intonarium Toletanum 1519,1520; 2 ejemplares del Diurnum 1519, 1520 que han sido descritos bibliográficamente y desde el punto de vista de las encuadernaciones así como también por su contenido musical. Es notable señalar que estás melodías estaban en circulación en época de Antonio de Cabezón, célebre compositor y organista español, quien pudo reparar en algunas de estas melodías para inspirarse en sus composiciones

  15. étude par AFM dynamique.

    African Journals Online (AJOL)

    user

    Ces forces oscillatoires de solvatation sont carac- térisées par une augmentation en amplitude lorsque la distance de confinement diminue (en tendant vers zéro), la périodicité des oscillations étant approximativement égales au diamètre des molécules du liquide confiné. Dans ce travail, nous sommes partis des récents ...

  16. Proteinase-activated receptors (PARs) as targets for antiplatelet therapy.

    Science.gov (United States)

    Cunningham, Margaret; McIntosh, Kathryn; Bushell, Trevor; Sloan, Graeme; Plevin, Robin

    2016-04-15

    Since the identification of the proteinase-activated receptor (PAR) family as mediators of serine protease activity in the 1990s, there has been tremendous progress in the elucidation of their pathophysiological roles. The development of drugs that target PARs has been the focus of many laboratories for the potential treatment of thrombosis, cancer and other inflammatory diseases. Understanding the mechanisms of PAR activation and G protein signalling pathways evoked in response to the growing list of endogenous proteases has yielded great insight into receptor regulation at the molecular level. This has led to the development of new selective modulators of PAR activity, particularly PAR1. The mixed success of targeting PARs has been best exemplified in the context of inhibiting PAR1 as a new antiplatelet therapy. The development of the competitive PAR1 antagonist, vorapaxar (Zontivity), has clearly shown the value in targeting PAR1 in acute coronary syndrome (ACS); however the severity of associated bleeding with this drug has limited its use in the clinic. Due to the efficacy of thrombin acting via PAR1, strategies to selectively inhibit specific PAR1-mediated G protein signalling pathways or to target the second thrombin platelet receptor, PAR4, are being devised. The rationale behind these alternative approaches is to bias downstream thrombin activity via PARs to allow for inhibition of pro-thrombotic pathways but maintain other pathways that may preserve haemostatic balance and improve bleeding profiles for widespread clinical use. This review summarizes the structural determinants that regulate PARs and the modulators of PAR activity developed to date. © 2016 Authors; published by Portland Press Limited.

  17. Regulatory cross-talk in the double par locus of plasmid pB171

    DEFF Research Database (Denmark)

    Ringgaard, Simon; Ebersbach, Gitte; Borch, Jonas

    2007-01-01

    The double par locus of Escherichia coli virulence factor pB171 consists of two adjacent and oppositely oriented par loci of different types, called par1 and par2. par1 encodes an actin ATPase (ParM), and par2 encodes an oscillating, MinD-like ATPase (ParA). The par loci share a central cis-actin...... well with the observed transcriptional regulation of the par operons in vivo and in vitro. Integration host factor (IHF) was identified as a novel factor involved in par2-mediated plasmid partitioning....

  18. Diagnosis of Parietaria judaica pollen allergy using natural and recombinant Par j 1 and Par j 2 allergens.

    Science.gov (United States)

    González-Rioja, R; Ferrer, A; Arilla, M C; Ibarrola, I; Viguera, A R; Andreu, C; Martínez, A; Asturias, J A

    2007-02-01

    Parietaria judaica pollen is one of the main causes of allergic diseases in the Mediterranean area and contains two major allergens, called Par j 1 and Par j 2. To evaluate the diagnostic potential of natural and recombinant forms of Par j 1 and Par j 2 in comparison with standardized P. judaica pollen extract. Thirty patients allergic to P. judaica pollen and 15 control patients were investigated. Skin prick tests and determination of specific IgE levels were performed with commercial P. judaica extract, natural Par j 1 and Par j 2, and recombinant forms of both allergens expressed in P. pastoris. The whole group of patients with allergy to P. judaica had a positive skin test reaction to purified nPar j 1-Par j 2 and rPar j 2 at 5 microg/mL, and no false-positive reactions were detected. Natural and recombinant Par j 1 and Par j 2 showed no significantly different responses in skin tests compared with P. judaica extract. A high correlation was found between the serum-specific IgE levels to P. judaica extract vs. natural (R=0.996; P<0.001) and recombinant allergens (R=0.887 and 0.982 for rPar j 1 and rPar j 2, respectively; P<0.001). rPar j 2 displayed a 100% sensitivity and specificity among P. judaica-allergic patients. In vivo and in vitro diagnosis of P. judaica pollen allergy could be simplified using rPar j 2. This protein showed comparable IgE response and skin prick reactivity with those produced by P. judaica pollen extract.

  19. Aproximación crítica al pensamiento dicotómico “indios amigos” versus “indios enemigos” bajo el gobierno del Marqués de Baides, Chile,1639-1646

    Directory of Open Access Journals (Sweden)

    Jimena Obregón Iturra

    2008-07-01

    Full Text Available En este artículo nos proponemos cuestionar la diferenciación binaria entre indios amigos e indios enemigos, ampliamente difundida durante el siglo XVII para calificar a los distintos grupos araucano-mapuches del sur de Chile. Restringiendo el análisis al funcionamiento de esta categorización dual bajo el gobierno del Marqués de Baides, mostramos cómo el primer Parlamento de Quillín enuncia y consagra la partición entre los “enemigos” ausentes del parlamento y los “amigos” que han dado las paces, haciendo desaparecer los términos medios. Paralelamente, planteamos que retomar de manera acrítica categorías heredadas del pasado colonial, que además conllevan tal carga ideológica, dificulta la comprensión de una compleja realidad fronteriza que dista mucho de ser dicotómica. Finalmente, recalcamos los arduos problemas plan- teados por la polémica figura de Carl Schmitt que, a lo largo del siglo XX, dejó su engorrosa impronta en la conceptualización amigos/enemigos.

  20. Lope and the Creation of Contemporary Heroes: «La nueva victoria de don Gonzalo de Córdoba» y «La nueva victoria del marqués de Santa Cruz»

    Directory of Open Access Journals (Sweden)

    Teresa Ferrer Valls

    2013-05-01

    Full Text Available Lope defended the usefulness of history presented on stage, that is, theatre’s function of preserving and creating a collective memory. In the case of La nueva victoria de don Gonzalo and La nueva victoria del marqués de Santa Cruz, this playwright dramatized military events that took place immediately before the composition of these plays. In this article i will analyze the fundamental traits of those texts in relation to Lope’s desire to please his patrons and, at the same time, to participate in the making of a Spanish national identity, which intensified during the first years of king Philip IV’s reign through different media, among them painting and theatre. This paper ends reflecting on the risk assumed by the playwright when dealing with recent political events. This is exemplified by the existing news about the prohibition of a play by Lope, nowadays lost, related to the death of the king of Sweden, in which the protagonists of the two aforementioned plays are also referenced.

  1. A single parS sequence from the cluster of four sites closest to oriC is necessary and sufficient for proper chromosome segregation in Pseudomonas aeruginosa.

    Directory of Open Access Journals (Sweden)

    Paulina Jecz

    Full Text Available Among the mechanisms that control chromosome segregation in bacteria are highly-conserved partitioning systems comprising three components: ParA protein (a deviant Walker-type ATPase, ParB protein (a DNA-binding element and multiple cis-acting palindromic centromere-like sequences, designated parS. Ten putative parS sites have been identified in the P. aeruginosa PAO1 genome, four localized in close proximity of oriC and six, diverged by more than one nucleotide from a perfect palindromic sequence, dispersed along the chromosome. Here, we constructed and analyzed P. aeruginosa mutants deprived of each single parS sequence and their different combinations. The analysis included evaluation of a set of phenotypic features, chromosome segregation, and ParB localization in the cells. It was found that ParB binds specifically to all ten parS sites, although with different affinities. The P. aeruginosa parS mutant with all ten parS sites modified (parSnull is viable however it demonstrates the phenotype characteristic for parAnull or parBnull mutants: slightly slower growth rate, high frequency of anucleate cells, and defects in motility. The genomic position and sequence of parS determine its role in P. aeruginosa biology. It transpired that any one of the four parS sites proximal to oriC (parS1 to parS4, which are bound by ParB with the highest affinity, is necessary and sufficient for the parABS role in chromosome partitioning. When all these four sites are mutated simultaneously, the strain shows the parSnull phenotype, which indicates that none of the remaining six parS sites can substitute for these four oriC-proximal sites in this function. A single ectopic parS2 (inserted opposite oriC in the parSnull mutant facilitates ParB organization into regularly spaced condensed foci and reverses some of the mutant phenotypes but is not sufficient for accurate chromosome segregation.

  2. A single parS sequence from the cluster of four sites closest to oriC is necessary and sufficient for proper chromosome segregation in Pseudomonas aeruginosa.

    Science.gov (United States)

    Jecz, Paulina; Bartosik, Aneta A; Glabski, Krzysztof; Jagura-Burdzy, Grazyna

    2015-01-01

    Among the mechanisms that control chromosome segregation in bacteria are highly-conserved partitioning systems comprising three components: ParA protein (a deviant Walker-type ATPase), ParB protein (a DNA-binding element) and multiple cis-acting palindromic centromere-like sequences, designated parS. Ten putative parS sites have been identified in the P. aeruginosa PAO1 genome, four localized in close proximity of oriC and six, diverged by more than one nucleotide from a perfect palindromic sequence, dispersed along the chromosome. Here, we constructed and analyzed P. aeruginosa mutants deprived of each single parS sequence and their different combinations. The analysis included evaluation of a set of phenotypic features, chromosome segregation, and ParB localization in the cells. It was found that ParB binds specifically to all ten parS sites, although with different affinities. The P. aeruginosa parS mutant with all ten parS sites modified (parSnull) is viable however it demonstrates the phenotype characteristic for parAnull or parBnull mutants: slightly slower growth rate, high frequency of anucleate cells, and defects in motility. The genomic position and sequence of parS determine its role in P. aeruginosa biology. It transpired that any one of the four parS sites proximal to oriC (parS1 to parS4), which are bound by ParB with the highest affinity, is necessary and sufficient for the parABS role in chromosome partitioning. When all these four sites are mutated simultaneously, the strain shows the parSnull phenotype, which indicates that none of the remaining six parS sites can substitute for these four oriC-proximal sites in this function. A single ectopic parS2 (inserted opposite oriC in the parSnull mutant) facilitates ParB organization into regularly spaced condensed foci and reverses some of the mutant phenotypes but is not sufficient for accurate chromosome segregation.

  3. Regulatory cross-talk in the double par locus of plasmid pB171

    DEFF Research Database (Denmark)

    Ringgaard, Simon; Ebersbach, Gitte; Borch, Jonas

    2007-01-01

    The double par locus of Escherichia coli virulence factor pB171 consists of two adjacent and oppositely oriented par loci of different types, called par1 and par2. par1 encodes an actin ATPase (ParM), and par2 encodes an oscillating, MinD-like ATPase (ParA). The par loci share a central cis......-acting region of approximately 200 bp, called parC1, located between the two par loci. An additional cis-acting region, parC2, is located downstream of the parAB operon of par2. Here we show that ParR of par1 and ParB of par2 bind cooperatively to unrelated sets of direct repeats in parC1 to form the cognate...... partition and promoter repression complexes. Surprisingly, ParB repressed transcription of the noncognate par operon, indicating cross-talk and possibly epistasis between the two systems. The par promoters, P1 and P2, affected each other negatively. The DNA binding activities of ParR and ParB correlated...

  4. ÉTUDE DE CAS — Brésil : La contamination par le mercure en ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    11 janv. 2011 ... Leishmaniose : maladie sans frontières. Une mouche porteuse d'un parasite mortel hante la jungle des chutes d'Iguazu, qui marque la triple frontière entre le Brésil, l'Argentine et le. Voir davantageLeishmaniose : maladie sans frontières ...

  5. Ionisation D'atomes Par un Laser Carbon Dioxide Intense Par L'effet Tunnel

    Science.gov (United States)

    Xiong, Wei

    Cette these decrit des experiences d'ionisation d'atomes (Xe et K) par un laser CO_2 intense. Des spectrometres de masse et d'energie electronique ont ete developpes pour mesurer le nombre d'ions crees et l'energie des electrons acceleres par le gradient spatial du champ laser. Des details sur ces mesures sont fournis et justifies. On a mesure des energies d'electrons jusqu'a 1000eV, a une intensite de 10^{14} W/cm^2. Les mesures experimentales sont comparees avec deux types de theorie, qui decrivent le processus d'ionisation dans deux limites: l'interaction entre electron et noyau est beaucoup plus forte que celle entre electron et champ laser (processus multiphotonique), ou l'inverse (effect tunnel). Les resultats montrent que la courbe du nombre d'ions crees en fonction de l'intensite n'est pas tres sensible aux details du processus d'ionsisation. Par contre les spectres d'energie des electrons dependent fortement du processus d'ionisation, et on peut en deduire le taux d'ionisation. On trouve que la theorie multiphotonique ne fournit pas une explication satisfaisante aux resultats experimentaux, mais que la theorie de l'effet tunnel s'ajuste bien si on suppose que le seuil d'ionisation peut etre deplace legerement par le champ laser. Le deplacement necessaire du seuil est estime dans cette these, mais une explication theorique reste a trouver.

  6. Cataract surgery after pars plana vitrectomy.

    Science.gov (United States)

    Shousha, Mohamed Abou; Yoo, Sonia H

    2010-01-01

    To review recent studies and advances and their possible implications in the care of patients undergoing cataract surgery after pars plana vitrectomy. Optical biometry has shown to be superior to ultrasound biometry in vitrectomized eyes but still not achieving as good results as it does in nonvitrectomized eyes. Blue light-filter intraocular lenses, with their possible advantage of macular protection, have shown no operative or functional disadvantages in vitrectomized eyes, and thus their routine use can be justified. However, presbyopia-correcting intraocular lenses, at least at their current stage of development, generally, are still not accepted for vitrectomized eyes. Combining cataract surgery with intravitreal injections of bevacizumab or triamcinolone acetonide in patients with macular edema and cataract is advisable to avoid exacerbation of the condition and improve visual outcome. Despite the recent advances, incidences of posterior capsular opacification and retinal detachment are still considerable. Understanding ocular anatomical alterations imposed by the previous pars plana vitrectomy surgery and the underlying vitreoretinal disease will allow the surgeon to address the special challenges. Despite that, recent advances in techniques and instrumentation have improved the surgical safety and outcomes, reported complications rates are still relatively high.

  7. A Par-1-Par-3-Centrosome Cell Polarity Pathway and Its Tuning for Isotropic Cell Adhesion.

    Science.gov (United States)

    Jiang, Tao; McKinley, R F Andrew; McGill, Melanie A; Angers, Stephane; Harris, Tony J C

    2015-10-19

    To form regulated barriers between body compartments, epithelial cells polarize into apical and basolateral domains and assemble adherens junctions (AJs). Despite close links with polarity networks that generate single polarized domains, AJs distribute isotropically around the cell circumference for adhesion with all neighboring cells [1-3]. How AJs avoid the influence of polarity networks to maintain their isotropy has been unclear. In established epithelia, trans cadherin interactions could maintain AJ isotropy [4], but AJs are dynamic during epithelial development and remodeling [5, 6], and thus specific mechanisms may control their isotropy. In Drosophila, aPKC prevents hyper-polarization of junctions as epithelia develop from cellularization to gastrulation [7]. Here, we show that aPKC does so by inhibiting a positive feedback loop between Bazooka (Baz)/Par-3, a junctional organizer [5, 8-10], and centrosomes. Without aPKC, Baz and centrosomes lose their isotropic distributions and recruit each other to single plasma membrane (PM) domains. Surprisingly, our loss- and gain-of-function analyses show that the Baz-centrosome positive feedback loop is driven by Par-1, a kinase known to phosphorylate Baz and inhibit its basolateral localization [8, 11, 12]. We find that Par-1 promotes the positive feedback loop through both centrosome microtubule effects and Baz phosphorylation. Normally, aPKC attenuates the circuit by expelling Par-1 from the apical domain at gastrulation. The combination of local activation and global inhibition is a common polarization strategy [13-16]. Par-1 seems to couple both effects for a potent Baz polarization mechanism that is regulated for the isotropy of Baz and AJs around the cell circumference. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. The PAR Proteins: Fundamental Players in Animal Cell Polarization

    OpenAIRE

    Goldstein, Bob; Macara, Ian G.

    2007-01-01

    The par genes were discovered in genetic screens for regulators of cytoplasmic partitioning in the early embryo of C. elegans, and encode six different proteins required for asymmetric cell division by the worm zygote. Some of the PAR proteins are localized asymmetrically and form physical complexes with one another. Strikingly, the PAR proteins have been found to regulate cell polarization in many different contexts in diverse animals, suggesting they form part of an ancient and fundamental ...

  9. Parálisis cerebral Cerebral palsy

    OpenAIRE

    Jorge Malagon Valdez

    2007-01-01

    El término parálisis cerebral (PC) engloba a un gran número de síndromes neurológicos clínicos, de etiología diversa. Estos síndromes se caracterizan por tener una sintomatología común: los trastornos motores. Algunos autores prefieren manejar términos como "encefalopatía fija", "encefalopatías no evolutivas". Se mencionan la utilidad de programas de intervención temprana y métodos especiales de rehabilitación, así como el manejo de las deficiencias asociadas como la epilepsia, deficiencia me...

  10. TMS suppression of right pars triangularis, but not pars opercularis, improves naming in aphasia

    Science.gov (United States)

    Naeser, Margaret A.; Martin, Paula I.; Theoret, Hugo; Kobayashi, Masahito; Fregni, Felipe; Nicholas, Marjorie; Tormos, Jose M.; Steven, Megan S.; Baker, Errol H.; Pascual-Leone, Alvaro

    2011-01-01

    This study sought to discover if an optimum 1 cm2 area in the non-damaged right hemisphere (RH) was present, which could temporarily improve naming in chronic, nonfluent aphasia patients when suppressed with repetitive transcranial magnetic stimulation (rTMS). Ten minutes of slow, 1 Hz rTMS was applied to suppress different RH ROIs in eight aphasia cases. Picture naming and response time (RT) were examined before, and immediately after rTMS. In aphasia patients, suppression of right pars triangularis (PTr) led to significant increase in pictures named, and significant decrease in RT. Suppression of right pars opercularis (POp), however, led to significant increase in RT, but no change in number of pictures named. Eight normals named all pictures correctly; similar to aphasia patients, RT significantly decreased following rTMS to suppress right PTr, versus right POp. Differential effects following suppression of right PTr versus right POp suggest different functional roles for these regions. PMID:21864891

  11. Transcriptome profiling reveals links between ParS/ParR, MexEF-OprN, and quorum sensing in the regulation of adaptation and virulence in Pseudomonas aeruginosa.

    Science.gov (United States)

    Wang, Dongping; Seeve, Candace; Pierson, Leland S; Pierson, Elizabeth A

    2013-09-13

    The ParS/ParR two component regulatory system plays critical roles for multidrug resistance in Pseudomonas aeruginosa. It was demonstrated that in the presence of antimicrobials, ParR enhances bacterial survival by distinct mechanisms including activation of the mexXY efflux genes, enhancement of lipopolysaccharide modification through the arn operon, and reduction of the expression of oprD porin. In this study, we report on transcriptomic analyses of P. aeruginosa PAO1 wild type and parS and parR mutants growing in a defined minimal medium. Our transcriptomic analysis provides the first estimates of transcript abundance for the 5570 coding genes in P. aeruginosa PAO1. Comparative transcriptomics of P. aeruginosa PAO1 and par mutants identified a total of 464 genes regulated by ParS and ParR. Results also showed that mutations in the parS/parR system abolished expression of the mexEF-oprN operon by down-regulating the regulatory gene mexS. In addition to the known effects on drug resistance genes, transcript abundances of the quorum sensing genes (rhlIR and pqsABCDE-phnAB) were higher in both parS and parR mutants. In accordance with these results, a significant portion of the ParS/ParR regulated genes belonged to the MexEF-OprN and quorum sensing regulons. Deletion of the par genes also led to increased phenazine production and swarming motility, consistent with the up-regulation of the phenazine and rhamnolipid biosynthetic genes, respectively. Our results link the ParS/ParR two component signal transduction system to MexEF-OprN and quorum sensing systems in P. aeruginosa. These results expand our understanding of the roles of the ParS/ParR system in the regulation of gene expression in P. aeruginosa, especially in the absence of antimicrobials.

  12. Movement and equipositioning of plasmids by ParA filament disassembly

    DEFF Research Database (Denmark)

    Ringgaard, Simon; van Zon, Jeroen; Howard, Martin

    2009-01-01

    Bacterial plasmids encode partitioning (par) loci that confer stable plasmid inheritance. We showed previously that, in the presence of ParB and parC encoded by the par2 locus of plasmid pB171, ParA formed cytoskeletal-like structures that dynamically relocated over the nucleoid. Simultaneously, ...... of the plasmids. Mathematical modeling of ParA and plasmid dynamics support these interpretations. Mutational analysis supports a molecular mechanism in which the ParB/parC complex controls ParA filament depolymerization....

  13. Pratique du bloc infraclaviculaire par voie sous coracoïdienne par ...

    African Journals Online (AJOL)

    Toutefois, des efforts pour réduire le temps de réalisation, choisir la meilleure réponse motrice distale sur laquelle injecter l'anesthésique local, par une pratique plus fréquente des techniques d'ALR périphérique, sont nécessaires pour augmenter le confort des patients et le taux de succès du bloc. Mots clés: Anesthésie ...

  14. Combined pars plana vitrectomy and pars plana Baerveldt tube placement in eyes with neovascular glaucoma.

    Science.gov (United States)

    Kolomeyer, Anton M; Seery, Christopher W; Emami-Naeimi, Parisa; Zarbin, Marco A; Fechtner, Robert D; Bhagat, Neelakshi

    2015-01-01

    To describe characteristics and outcomes of combined pars plana vitrectomy and Baerveldt tube insertion procedure from 2005 to 2010 in eyes with neovascular glaucoma. Seventy-nine patients (89 eyes) with ≥2 months of follow-up were included. Outcome measures were visual acuity, intraocular pressure (IOP), number of glaucoma medications, and complications. Changes in mean logMAR visual acuity, IOP, and glaucoma medications were compared by a two-tailed t-test. Mean patient age was 69.0 years. Forty-three (54%) were male. Mean follow-up time was 19.9 months. Most common causes of neovascular glaucoma was diabetes (n = 63 [71%]) and central retinal vein occlusion (n = 21 [24%]). Eighty-six eyes (97%) underwent a 250 mm Baerveldt drainage device and 3 (3.4%) a 350 mm Baerveldt. Forty-five (51%) 20-gauge, 12 (13%) 23-gauge, and 32 (36%) 25-gauge pars plana vitrectomies were performed. Fifty-two eyes (58%) preoperatively and 23 (33%) postoperatively received intraocular injections for rubeosis and macular edema. Mean ± standard deviation logMAR visual acuity at 18-, 24-, 36-, and 48-month follow-up time points was significantly better than preoperative vision (P pars plana vitrectomy eyes. Nine eyes (10%) required return to the operating room after combined procedure, including 4 eyes (4.5%) for retinal detachment and 3 (3.4%) for high IOP due to tube occlusion. Three eyes (3.4%) developed endophthalmitis and 2 (2.2%) progressed to being pre/phthisical (none were enucleated). Combined pars plana vitrectomy and Baerveldt glaucoma shunt may be a useful procedure in reducing IOP and number of glaucoma medications in eyes with neovascular glaucoma along with stabilizing visual acuity in a majority of these eyes. Further studies are warranted to verify and expand on these findings.

  15. Doxycycline directly targets PAR1 to suppress tumor progression.

    Science.gov (United States)

    Zhong, Weilong; Chen, Shuang; Zhang, Qiang; Xiao, Ting; Qin, Yuan; Gu, Ju; Sun, Bo; Liu, Yanrong; Jing, Xiangyan; Hu, Xuejiao; Zhang, Peng; Zhou, Honggang; Sun, Tao; Yang, Cheng

    2017-03-07

    Doxycycline have been reported to exert anti-cancer activity and have been assessed as anti-cancer agents in clinical trials. However, the direct targets of doxycycline in cancer cells remain unclear. In this study, we used a chemical proteomics approach to identify the Protease-activated receptor 1 (PAR1) as a specific target of inhibition of doxycycline. Binding assays and single-molecule imaging assays were performed to confirm the inhibition of doxycycline to PAR1. The effect of doxycycline on multi-omics and cell functions were assessed based on a PAR1/thrombin model. Molecular docking and molecular dynamic simulations revealed that doxycycline interacts with key amino acids in PAR1. Mutation of PAR1 further confirmed the computation-based results. Moreover, doxycycline provides highly selective inhibition of PAR1 signaling in tumors in vitro and in vivo. Using pathological clinical samples co-stained for doxycycline and PAR1, it was found that doxycycline fluorescence intensity and PAR1 expression shown a clear positive correlation. Thus, doxycycline may be a useful targeted anti-cancer drug that should be further investigated in clinical trials.

  16. Priapisme induit par la chlorpromazine: A propos de deux cas

    African Journals Online (AJOL)

    I. Marrag

    Nous rapportons ici le cas de deux patients qui ont présenté un priapisme en concomitance avec une pres- cription de chlorpromazine .... ischémiques pourraient être induits par des médicaments dont les neuroleptiques occupent ... vie sexuelle des patients, souvent touchée par la prescription des traitements psychotropes ...

  17. Bacterial mitosis: partitioning protein ParA oscillates in spiral-shaped structures and positions plasmids at mid-cell

    DEFF Research Database (Denmark)

    Ebersbach, Gitte; Gerdes, Kenn; Charbon, Gitte Ebersbach

    2004-01-01

    The par2 locus of Escherichia coli plasmid pB171 encodes oscillating ATPase ParA, DNA binding protein ParB and two cis-acting DNA regions to which ParB binds (parC1 and parC2). Three independent techniques were used to investigate the subcellular localization of plasmids carrying par2. In cells w...

  18. ParABS system in chromosome partitioning in the bacterium Myxococcus xanthus.

    Directory of Open Access Journals (Sweden)

    Antonio A Iniesta

    Full Text Available Chromosome segregation is an essential cellular function in eukaryotic and prokaryotic cells. The ParABS system is a fundamental player for a mitosis-like process in chromosome partitioning in many bacterial species. This work shows that the social bacterium Myxococcus xanthus also uses the ParABS system for chromosome segregation. Its large prokaryotic genome of 9.1 Mb contains 22 parS sequences near the origin of replication, and it is shown here that M. xanthus ParB binds preferentially to a consensus parS sequence in vitro. ParB and ParA are essential for cell viability in M. xanthus as in Caulobacter crescentus, but unlike in many other bacteria. Absence of ParB results in anucleate cells, chromosome segregation defects and loss of viability. Analysis of ParA subcellular localization shows that it clusters at the poles in all cells, and in some, in the DNA-free cell division plane between two chromosomal DNA masses. This ParA localization pattern depends on ParB but not on FtsZ. ParB inhibits the nonspecific interaction of ParA with DNA, and ParA colocalizes with chromosomal DNA only when ParB is depleted. The subcellular localization of ParB suggests a single ParB-parS complex localized at the edge of the nucleoid, next to a polar ParA cluster, with a second ParB-parS complex migrating after the replication of parS takes place to the opposite nucleoid edge, next to the other polar ParA cluster.

  19. Recombination in the human Pseudoautosomal region PAR1.

    Directory of Open Access Journals (Sweden)

    Anjali G Hinch

    2014-07-01

    Full Text Available The pseudoautosomal region (PAR is a short region of homology between the mammalian X and Y chromosomes, which has undergone rapid evolution. A crossover in the PAR is essential for the proper disjunction of X and Y chromosomes in male meiosis, and PAR deletion results in male sterility. This leads the human PAR with the obligatory crossover, PAR1, to having an exceptionally high male crossover rate, which is 17-fold higher than the genome-wide average. However, the mechanism by which this obligatory crossover occurs remains unknown, as does the fine-scale positioning of crossovers across this region. Recent research in mice has suggested that crossovers in PAR may be mediated independently of the protein PRDM9, which localises virtually all crossovers in the autosomes. To investigate recombination in this region, we construct the most fine-scale genetic map containing directly observed crossovers to date using African-American pedigrees. We leverage recombination rates inferred from the breakdown of linkage disequilibrium in human populations and investigate the signatures of DNA evolution due to recombination. Further, we identify direct PRDM9 binding sites using ChIP-seq in human cells. Using these independent lines of evidence, we show that, in contrast with mouse, PRDM9 does localise peaks of recombination in the human PAR1. We find that recombination is a far more rapid and intense driver of sequence evolution in PAR1 than it is on the autosomes. We also show that PAR1 hotspot activities differ significantly among human populations. Finally, we find evidence that PAR1 hotspot positions have changed between human and chimpanzee, with no evidence of sharing among the hottest hotspots. We anticipate that the genetic maps built and validated in this work will aid research on this vital and fascinating region of the genome.

  20. Megestrol acetate NCD oral suspension--Par Pharmaceutical: megestrol acetate nanocrystal dispersion oral suspension, PAR 100.2, PAR-100.2.

    Science.gov (United States)

    2007-01-01

    Par Pharmaceutical has developed megestrol acetate (Megace ES) oral suspension for the treatment of anorexia, cachexia and a significant weight loss associated with AIDS. Par Pharmaceutical used Elan Corporation's NanoCrystal Dispersion (NCD) technology to develop an advanced, concentrated formulation of megestrol acetate with improved bioavailability, more rapid onset of action, more convenient dosing and a lower dosing regimen compared with the original marketed formulation of megestrol acetate oral suspension. Patients are administered a teaspoon (5mL) of the new NCD formulation once daily, compared with a daily 20mL dosage cup of the original formulation. The new megestrol acetate NCD formulation represents a line-extension of Par's megestrol acetate oral suspension (800mg/20mL, Megace O/S) that has been marketed for anorexia, cachexia and AIDS-related weight loss since July 2001. Par's megestrol acetate is the generic version of Bristol-Myers Squibb's Megace Oral Suspension. NanoCrystal Dispersion (NCD) is a trademark of Elan Corporation. Par Pharmaceutical will market megestol acetate NCD oral suspension under the Megace brand name. The company licensed the Megace name from Bristol-Myers Squib in August 2003. The US FDA approved megestrol acetate oral suspension (625 mg/mL) in July 2005 for the treatment of anorexia, cachexia or a significant, unexplained weight loss in patients with AIDS. The NDA for the product was accepted for review by the agency in September 2004, following its submission in June of that year.Par Pharmaceutical commenced the first of two phase III clinical trials of megestrol acetate oral suspension (PAR 100.2) in cancer-induced anorexia in the first quarter of 2006. However, this trial was discontinued in September 2006 because of slow patient enrolment. The company intends to discuss future development options in this indication with the FDA.New formulations or dosage forms of megestrol acetate concentrated suspension are also in

  1. Effects of thrombin, PAR-1 activating peptide and a PAR-1 antagonist on umbilical artery resistance in vitro

    Directory of Open Access Journals (Sweden)

    Elliott John T

    2005-02-01

    Full Text Available Abstract Background The non-thrombotic effects of thrombin in cardiovascular tissues, as mediated via the protease activated receptors (PARs, and particularly PAR-1, have been the focus of much recent research. The aims of this study were to evaluate the effects of thrombin, a specific PAR-1 activating peptide (PAR1-AP, and a PAR-1 antagonist on human umbilical artery tone in vitro. Methods Human umbilical artery samples were obtained from 17 women at term. Arterial rings were suspended under physiologic conditions for isometric recording. The in vitro effects of thrombin (0.5 units/mL to 3 units/mL, PAR1-AP TFLLR-NH2 [10(-9 to 10(-6 M], and PAR-1 antagonist (N-trans cinnamoyl- p-fluoroPhe-p-guanidinoPhe-Leu-Arg-Orn-NH2 [10(-9 M to 10(-5 M] on umbilical artery tone were measured. Results Both thrombin and TFLLR-NH2 exerted a potent cumulative vasodilatory effect on human umbilical artery resistance (P 0.05. Conclusion These findings highlight a potential role for thrombin and PAR-1 receptors in vascular regulation of feto-placental blood flow in normal pregnancy, and in association with the vascular lesions associated with IUGR and pre-eclampsia.

  2. IGF-II receptors in luminal and basolateral membranes isolated from pars convoluta and pars recta of rabbit proximal tubule

    DEFF Research Database (Denmark)

    Jacobsen, Christian; Jessen, H; Flyvbjerg, A

    1995-01-01

    The binding of 125I-labeled insulin-like growth factor-II (125I-IGF-II) to luminal and basolateral membrane vesicles isolated from pars convoluta and the straight part (pars recta) of rabbit proximal tubule was investigated. Analyses of the binding data by use of the general stoichiometric bindin...

  3. Improvements to the properties of uranium by addition of small quantities of other metals; Ameliorations apportees a l'uranium par de faibles additions metalliques

    Energy Technology Data Exchange (ETDEWEB)

    Englander, M. [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1960-07-01

    enrichi en isotope U{sup 235}. Dans les conditions optimales de fonctionnement, un meme materiau combustible doit alors fournir un minimum de 3000 MWj/t, soit 72.10{sup 6} kWh par tonne d'uranium naturel, tout en etant porte a une temperature suffisante pour jouer efficacement son role de source de chaleur (entre 350 et 550 deg. C minimum). Or d'une facon assez surprenante, les agregats polycristallins des billettes d'uranium obtenues par coulee sous vide, ou apres filage a haute temperature, se presentent comme un ensemble de grains tres grossiers, a contours dechiquetes et irreguliers, et comportent de nombreuses marques de deformation intragranulaire (macles, lignes de glissement), accompagnees de fortes sous-structures. En plus, le spectre de la dimension des grains s'etend de quelques microns a quelques millimetres, selon les plages micrographiques examinees. Sous irradiation a ces temperatures, l'uranium metallique pur en barreaux cylindriques de diametre de l'ordre du pouce, se deforme: il se produit des fissures dans la masse, des variations de dimensions longitudinales et transversales (qui se traduisent par des peaux d'orange), soit par croissances plus ou moins directionnelles, soit par deformations superficielles qui peuvent provoquer des ruptures du materiau, de sa gaine ou des fleches suffisamment importantes pour obstruer les canaux de refroidissement. Il a ete reconnu par la suite que cette instabilite, sous l'effet des contraintes thermiques d'origine nucleaire, est due a la morphologie heterogene de l'uranium et a sa structure cristalline anisotrope (U{sub {alpha}} ou U{sub {beta}}). (auteur)

  4. Marques d'oralitat en els xats

    Directory of Open Access Journals (Sweden)

    Marta Torres i Vilatarsana

    2014-06-01

    features which are characteristic of the oral discourse, as Torres and Cuenca (2007 state. We have chosen as corpus of analysis chats which present a low level of formality and which are considered to be representative of the model of language used there. The challenge is focused on revealing how aspects of colloquial orality are present in written texts. The article aims at breaking with some topics associated to chat language: although formal transgression is evident, the level of grammatical respect is similar to colloquial oral discourse.

  5. Par j 1 and Par j 2, the major allergens from Parietaria judaica pollen, have similar immunoglobulin E epitopes.

    Science.gov (United States)

    Asturias, J A; Gómez-Bayón, N; Eseverri, J L; Martínez, A

    2003-04-01

    Parietaria judaica is the main cause of allergy in Mediterranean countries. The major allergens from P. judaica pollen, Par j 1 and Par j 2, have amino acid sequence identity of 45% and contain eight cysteine residues involved in disulphide bonds that compact the structure. The aim of the study was to identify IgE-binding epitopes on Par j 1 and Par j 2, the major allergens from P. judaica pollen and correlate them with the three-dimensional structure of the proteins. Overlapping peptides representing the complete length of Par j 1 and Par j 2 were synthesized on a cellulose-derivatized membrane. Sera from 17 P. judaica-allergenic patients were used to identify IgE-binding epitopes. Homology modelling of the three dimensional structure of both allergens was generated using the Swiss-Model software on the basis of previously reported crystal structures. Five and eight IgE-binding epitopes were identified on Par j 1 and Par j 2 allergens, respectively. Both proteins belonged to the non-specific lipid transfer proteins (ns-LTP) family and therefore a three-dimensional model of both allergens was constructed on the basis of the maize ns-LTP crystal structure. Par j 1 and Par j 2 allergens have three similar allergenic epitopes with high homology and identical conformation. Three similar IgE-epitopes of major allergens from P. judaica have been described. They could be good candidates for designing of IgE haptens as therapeutic agents with reduced anaphylactic side-effects or for developing hypoallergenic variants of these major allergens.

  6. Estratigrafía, evolución estructural y restauración. El caso de la iglesia de Santa Eulalia en Marquínez (Álava

    Directory of Open Access Journals (Sweden)

    Cámara, Leandro

    2010-12-01

    breve revisión de la teoría de estados límite y su aplicación a las estructuras de fábrica y a la iglesia de Santa Eulalia de Marquínez en particular; en paralelo se presenta el resultado del estudio histórico de su evolución constructiva; y se conjugan las conclusiones de ambos análisis en una secuencia temporal de la evolución estructural, una revisión de su seguridad y una propuesta de intervención

  7. Scleral Buckling for Rhegmatogenous Retinal Detachment Associated with Pars Planitis.

    Science.gov (United States)

    Kim, Yong-Kyu; Yoon, Wontae; Ahn, Jae Kyoun; Park, Sung Pyo

    2016-01-01

    Purpose. To evaluate the surgical outcome of scleral buckling (SB) in rhegmatogenous retinal detachment (RRD) patients associated with pars planitis. Methods. Retrospective review of RRD patients (32 eyes of pars planitis RRD and 180 eyes of primary RRD) who underwent SB. We compared primary and final anatomical success rates and visual outcomes between two groups. Results. Primary and final anatomical success were achieved in 25 (78.1%) and 31 (96.8%) eyes in the pars planitis RRD group and in 167 eyes (92.7%) and 176 eyes (97.7%) in primary RRD group, respectively. Both groups showed significant visual improvement (p Pars planitis RRD group was associated with higher rate of postoperative proliferative vitreoretinopathy (PVR) development (12.5% versus 2.8%, p = 0.031). Pars planitis and high myopia were significant preoperative risk factors and pseudophakia was borderline risk for primary anatomical failure after adjusting for various clinical factors. Conclusions. Pars planitis associated RRD showed inferior primary anatomical outcome after SB due to postoperative PVR development. However, final anatomical and visual outcomes were favorable. RRD cases associated with pars planitis, high myopia, and pseudophakia might benefit from different surgical approaches, such as combined vitrectomy and SB.

  8. Scleral Buckling for Rhegmatogenous Retinal Detachment Associated with Pars Planitis

    Directory of Open Access Journals (Sweden)

    Yong-Kyu Kim

    2016-01-01

    Full Text Available Purpose. To evaluate the surgical outcome of scleral buckling (SB in rhegmatogenous retinal detachment (RRD patients associated with pars planitis. Methods. Retrospective review of RRD patients (32 eyes of pars planitis RRD and 180 eyes of primary RRD who underwent SB. We compared primary and final anatomical success rates and visual outcomes between two groups. Results. Primary and final anatomical success were achieved in 25 (78.1% and 31 (96.8% eyes in the pars planitis RRD group and in 167 eyes (92.7% and 176 eyes (97.7% in primary RRD group, respectively. Both groups showed significant visual improvement (p<0.001 and there were no significant differences in final visual acuity. Pars planitis RRD group was associated with higher rate of postoperative proliferative vitreoretinopathy (PVR development (12.5% versus 2.8%, p=0.031. Pars planitis and high myopia were significant preoperative risk factors and pseudophakia was borderline risk for primary anatomical failure after adjusting for various clinical factors. Conclusions. Pars planitis associated RRD showed inferior primary anatomical outcome after SB due to postoperative PVR development. However, final anatomical and visual outcomes were favorable. RRD cases associated with pars planitis, high myopia, and pseudophakia might benefit from different surgical approaches, such as combined vitrectomy and SB.

  9. Multi-scale photoacoustic remote sensing (PARS) (Conference Presentation)

    Science.gov (United States)

    Haji Reza, Parsin; Bell, Kevan; Shi, W.; Zemp, Roger J.

    2016-03-01

    We introduce a novel multi-scale photoacoustic remote sensing (PARS) imaging system. Our system can provide optical resolution details for superficial structures as well as acoustic resolution for deep-tissue imaging down to 5 cm, in a non-contact setting. PARS system does not require any contact with the sample or ultrasound coupling medium. The optical resolution PARS (OR-OARS) system uses optically focused pulsed excitation with optical detection of photoacoustic signatures using a long-coherence interrogation beam co-focused and co-scanned with the excitation spot. In the OR-PARS initial pressures are sampled right at their subsurface origin where acoustic pressures are largest. The Acoustic resolution PARS (AR-PARS) picks up the surface oscillation of the tissue caused by generated photoacoustic signal using a modified version of Michelson interferometry. By taking advantage of 4-meters polarization maintaining single-mode fiber and a green fiber laser we have generated a multi-wavelength source using stimulated Raman scattering. Remote functional imaging using this multi-wavelength excitation source and PARS detection mechanism has been demonstrated. The oxygen saturation estimations are shown for both phantom and in vivo studies. Images of blood vessel structures for an In vivo chicken embryo model is demonstrated. The Phantom studies indicates ~3µm and ~300µm lateral resolution for OR-PARS and AR-PARS respectively. To the best of our knowledge this is the first dual modality non-contact optical and acoustic resolution system used for in vivo imaging.

  10. Quantitation of the major allergen of several Parietaria pollens by an anti-Par 1 monoclonal antibody-based ELISA. Analysis of crossreactivity among purified Par j 1, Par o 1 and Par m 1 allergens.

    Science.gov (United States)

    Ayuso, R; Carreira, J; Polo, F

    1995-10-01

    Plants of the genus Parietaria, Urticaceae family, represent a major cause of pollinosis in the Mediterranean area. Different Parietaria species crossreact to a great extent, but studies on the crossreactivity among the major allergens of these pollens have not been carried out so far. To develop an immunochemical method to quantify the major Parietaria judaica allergen, Par j 1, as well as to verify the presence of Par j 1-like proteins in different Urticaceae pollens. These proteins would be purified in order to study the cross-reactivity among them. Immunoaffinity chromatography with a monoclonal antibody, solid-phase enzyme-linked immunoassays and SDS-PAGE. A monoclonal antibody-based ELISA for the quantification of Par j 1 has been developed. The assay has a sensitivity of 0.2 ng/mL and shows a high correlation with the allergenic activity of P. judaica extracts determined by radioallergosorbent assay (RAST) inhibition. By means of this assay, proteins homologous to Par j 1 were detected in P. officinalis and P. mauritanica. These proteins (Par o 1 and Par m 1, respectively) were purified by affinity chromatography using the same monoclonal antibody employed in the ELISA. Crossed-inhibition experiments demonstrated that Par j 1, Par o 1, and Par m 1, competed for the binding of specific IgE from a P. judaica-sensitive patients serum pool. The results here described suggest that shared allergenic epitopes are present in the three main allergens investigated, which may simplify the diagnosis and therapy for Parietaria allergy.

  11. uPAR as anti-cancer target

    DEFF Research Database (Denmark)

    Lund, Ida K; Illemann, Martin; Thurison, Tine

    2011-01-01

    , and a potential diagnostic and predictive impact of the different uPAR forms has been reported. Hence, pericellular proteolysis seems to be a suitable target for anti-cancer therapy and numerous approaches have been pursued. Targeting of this process may be achieved by preventing the binding of uPA to u...... using mouse monoclonal antibodies (mAbs) against mouse uPA or uPAR. These reagents will target uPA and uPAR in both stromal cells and cancer cells, and their therapeutic potential can now be assessed in syngenic mouse cancer models....

  12. The inflammatory marker suPAR after cardiac arrest

    DEFF Research Database (Denmark)

    Rundgren, Malin; Lyngbaek, Stig; Fisker, Helle

    2015-01-01

    BACKGROUND: Soluble urokinase plasminogen activator receptor (suPAR) is released in response to inflammatory stimuli, and plasma levels are associated with long-term outcomes. The ischemia/reperfusion injury caused by cardiac arrest (CA) and resuscitation triggers an inflammatory response...... analysis shoved an AUC of 0.76 at 6 hours. In the subgroup of CA of cardiac cause, the AUC was 0.84. CONCLUSION: suPAR levels at 6 and 36 hours after CA were significantly higher in nonsurviving patients compared with survivors; however, the overlap in suPAR levels between the outcome groups...

  13. Par j 1 and Par j 2, the two major allergens in Parietaria judaica, bind preferentially to monoacylated negative lipids.

    Science.gov (United States)

    González-Rioja, Roberto; Asturias, Juan A; Martínez, Alberto; Goñi, Félix M; Viguera, Ana Rosa

    2009-03-01

    Par j 1 and Par j 2 proteins are the two major allergens in Parietaria judaica pollen, one of the main causes of allergic diseases in the Mediterranean area. Each of them contains eight cysteine residues organized in a pattern identical to that found in plant nonspecific lipid transfer proteins. The 139- and 102-residue recombinant allergens, corresponding respectively to Par j 1 and Par j 2, refold properly to fully functional forms, whose immunological properties resemble those of the molecules purified from the natural source. Molecular modeling shows that, despite the lack of extensive primary structure homology with nonspecific lipid transfer proteins, both allergens contain a hydrophobic cavity suited to accommodate a lipid ligand. In the present study, we present novel evidence for the formation of complexes of these natural and recombinant proteins from Parietaria pollen with lipidic molecules. The dissociation constant of oleyl-lyso-phosphatidylcholine is 9.1 +/- 1.2 microm for recombinant Par j 1, whereas pyrenedodecanoic acid shows a much higher affinity, with a dissociation constant of approximately 1 microm for both recombinant proteins, as well as for the natural mixture. Lipid binding does not alter the secondary structure content of the protein but is very efficient in protecting disulfide bonds from reduction by dithiothreitol. We show that Par j 1 and Par j 2 not only bind lipids from micellar dispersions, but also are able to extract and transfer negative phospholipids from bilayers.

  14. Parálisis cerebral Cerebral palsy

    Directory of Open Access Journals (Sweden)

    Jorge Malagon Valdez

    2007-01-01

    Full Text Available El término parálisis cerebral (PC engloba a un gran número de síndromes neurológicos clínicos, de etiología diversa. Estos síndromes se caracterizan por tener una sintomatología común: los trastornos motores. Algunos autores prefieren manejar términos como "encefalopatía fija", "encefalopatías no evolutivas". Se mencionan la utilidad de programas de intervención temprana y métodos especiales de rehabilitación, así como el manejo de las deficiencias asociadas como la epilepsia, deficiencia mental, trastornos del lenguaje, audición, visión, déficit de la atención que mejoran el pronóstico de manera significativa. El pronóstico también depende de la gravedad del padecimiento y de las manifestaciones asociadas.The term cerebral palsy (CP, is used for a great number of clinical neurological syndromes. The syndromes are characterized by having a common cause, motor defects. It is important, because they can cause a brain damage by presenting motor defects and some associated deficiencies, such as mental deficiency, epilepsy, language and visual defects and pseudobulbar paralysis, with the nonevolving fact. Some authors prefer using terms such as "non-evolving encephalopathies". In the treatment the utility of prevention programs of early stimulation and special rehabilitation methods, and treatment of associated deficiencies such as epilepsy, mental deficiency, language, audition and visual problems, and the attention deficit improve the prognosis in an important way. The prognosis depends on the severity of the disease and the associated manifestations.

  15. Elimination du cadmium de l'acide phosphorique par électrodialyse

    African Journals Online (AJOL)

    spectromètre d'absorption atomique de marque. Shimadzu AA 6200. Figure 1. Montage de l'installation d'électrodialyse avec une cellule à quatre compartiments. 1 : cellule à quatre compartiments. 2 : alimentation de courant. 3 : ampèremètre. 4 : pompe péristaltique. 5 : réservoir de solutions. 6 : agitateurs magnétiques.

  16. Bovine pars tuberalis secretions release growth hormone from rat pars distalis of pituitary gland.

    Science.gov (United States)

    Lafarque, Martha María; Ezquer, Marcelo; Aguado, Luis Inocencio; Oliveros, Liliana Beatriz

    2004-08-01

    The pituitary pars tuberalis (PT) is characterized by PT-specific secretory cells which have raised the possibility of an endocrine function for this portion of adenohypophysis. To investigate the effect of the secretion of bovine PT cells into culture medium on growth hormone (GH) response of dispersed pars distalis (PD) cells of rats. 48-hour culture medium of all PT cells at 1 microg of protein concentration induced the greatest GH release from PD cells. After PT cells separation on a discontinuos Percoll gradient and culturing, only the culture medium of cells from 50 and 60% Percoll strength released GH from PD cells. Therefore, cells from 50 and 60% strenght Percoll were cultured together. Only 0.2 mg protein of this culture medium was required to induce the maximal GH release from PD cells, suggesting that these cells could be responsible for producing the factor(s) which affect PD somatotrophe cells. After protein separation by 12% SDS-PAGE of this PT culture medium bands were eluted. The biological activity, measured as ng/ml of GH from PD cells, corresponded to a protein(s) of molecular weight between 45 and 66 kDal. The results indicate that there is an active proteic factor(s) secreted by the PT that acts upon PD cells to stimulate GH release and that PD could be an effector organ for some secretory product(s) of the PT.

  17. Récolte et consommation de pollen par les abeilles

    OpenAIRE

    Odoux, Jean Francois

    2005-01-01

    La récolte de pollen par les abeilles fait appel à des organes spécialisés de l'abeille, comme celle du nectar. Par contre, elle est régie par de très nombreux facteurs, en fonction des ressources, bien sûr, mais aussi des besoins de la colonie, alors que son attractivité n'est pas toujours en rapport avec la qualité. Ce texte décrit le contexte de l'utilisation du pollen par les abeilles, de la récolte à la digestion, d'après une synthèse bibliographique.

  18. PARS: Programs for Analysis and Resizing of Structures, user manual

    Science.gov (United States)

    Haftka, R. T.; Prasad, B.; Tsach, U.

    1979-01-01

    PARS processors and their use, flutter analysis, sensitivity analysis for stresses, and resizing are presented. Design variable definition and interface with finite element model, static constraints and their derivatives, flutter derivatives, and optimization are discussed.

  19. First-in-human uPAR PET

    DEFF Research Database (Denmark)

    Persson, Morten; Skovgaard, Dorthe; Brandt-Larsen, Malene

    2015-01-01

    A first-in-human clinical trial with Positron Emission Tomography (PET) imaging of the urokinase-type plasminogen activator receptor (uPAR) in patients with breast, prostate and bladder cancer, is described. uPAR is expressed in many types of human cancers and the expression is predictive...... of invasion, metastasis and indicates poor prognosis. uPAR PET imaging therefore holds promise to be a new and innovative method for improved cancer diagnosis, staging and individual risk stratification. The uPAR specific peptide AE105 was conjugated to the macrocyclic chelator DOTA and labeled with (64)Cu...... for targeted molecular imaging with PET. The safety, pharmacokinetic, biodistribution profile and radiation dosimetry after a single intravenous dose of (64)Cu-DOTA-AE105 were assessed by serial PET and computed tomography (CT) in 4 prostate, 3 breast and 3 bladder cancer patients. Safety assessment...

  20. Peptide-Based Optical uPAR Imaging for Surgery

    DEFF Research Database (Denmark)

    Juhl, Karina; Christensen, Anders; Persson, Morten

    2016-01-01

    Near infrared intra-operative optical imaging is an emerging technique with clear implications for improved cancer surgery by enabling a more distinct delineation of the tumor margins during resection. This modality has the potential to increase the number of patients having a curative radical...... tumor resection. In the present study, a new uPAR-targeted fluorescent probe was developed and the in vivo applicability was evaluated in a human xenograft mouse model. Most human carcinomas express high level of uPAR in the tumor-stromal interface of invasive lesions and uPAR is therefore considered...... an ideal target for intra-operative imaging. Conjugation of the flourophor indocyanine green (ICG) to the uPAR agonist (AE105) provides an optical imaging ligand with sufficiently high receptor affinity to allow for a specific receptor targeting in vivo. For in vivo testing, human glioblastoma xenograft...

  1. Croissance epitaxiale de GaAs sur substrats de Ge par epitaxie par faisceaux chimiques

    Science.gov (United States)

    Belanger, Simon

    La situation energetique et les enjeux environnementaux auxquels la societe est confrontee entrainent un interet grandissant pour la production d'electricite a partir de l'energie solaire. Parmi les technologies actuellement disponibles, la filiere du photovoltaique a concentrateur solaire (CPV pour concentrator photovoltaics) possede un rendement superieur et mi potentiel interessant a condition que ses couts de production soient competitifs. La methode d'epitaxie par faisceaux chimiques (CBE pour chemical beam epitaxy) possede plusieurs caracteristiques qui la rendent interessante pour la production a grande echelle de cellules photovoltaiques a jonctions multiples a base de semi-conducteurs III-V. Ce type de cellule possede la meilleure efficacite atteinte a ce jour et est utilise sur les satellites et les systemes photovoltaiques a concentrateur solaire (CPV) les plus efficaces. Une des principales forces de la technique CBE se trouve dans son potentiel d'efficacite d'utilisation des materiaux source qui est superieur a celui de la technique d'epitaxie qui est couramment utilisee pour la production a grande echelle de ces cellules. Ce memoire de maitrise presente les travaux effectues dans le but d'evaluer le potentiel de la technique CBE pour realiser la croissance de couches de GaAs sur des substrats de Ge. Cette croissance constitue la premiere etape de fabrication de nombreux modeles de cellules solaires a haute performance decrites plus haut. La realisation de ce projet a necessite le developpement d'un procede de preparation de surface pour les substrats de germanium, la realisation de nombreuses sceances de croissance epitaxiale et la caracterisation des materiaux obtenus par microscopie optique, microscopie a force atomique (AFM), diffraction des rayons-X a haute resolution (HRXRD), microscopie electronique a transmission (TEM), photoluminescence a basse temperature (LTPL) et spectrometrie de masse des ions secondaires (SIMS). Les experiences ont permis

  2. Differences in middle ear ventilation disorders between pars flaccida and pars tensa cholesteatoma in sonotubometry and patterns of tympanic and mastoid pneumatization.

    Science.gov (United States)

    Shinnabe, Akihiro; Hara, Mariko; Hasegawa, Masayo; Matsuzawa, Shingo; Kanazawa, Hiromi; Kanazawa, Takeharu; Yoshida, Naohiro; Iino, Yukiko

    2012-07-01

    To investigate differences in middle ear ventilation mechanisms between pars flaccida and pars tensa cholesteatoma. Retrospective case review. A referral hospital otolaryngology department. Sixty-six ears with pars flaccida cholesteatoma (mean age, 45.7 yr) and 19 ears with pars tensa cholesteatoma (mean age, 58.8 yr) were included. Patients with totally adhesive tympanic membranes or mixed-type cholesteatoma were excluded. Patients underwent canal wall down tympanoplasty with canal reconstruction. The canal wall was reconstructed with tragal or conchal cartilage and cortical bone grafts. Habitual sniffing, preoperative sonotubometry results, mastoid pneumatization (maturation), and postoperative aeration around the stapes were investigated. Preoperative mastoid pneumatization and postoperative aeration around the stapes were measured on computed tomography scans. Ten (15.4%) of 65 patients with pars flaccida cholesteatoma and 3 (15.7%) of 19 patients with pars tensa cholesteatoma were habitual sniffers (p = 0.5). Preoperative sonotubometry indicated that a patulous pattern was more common in ears with pars flaccida than pars tensa cholesteatoma (42.8% versus 7.1%, p pars tensa than pars flaccida cholesteatoma (85.8% versus 42.8%, p pars flaccida than pars tensa cholesteatoma (p pars flaccida and pars tensa cholesteatoma.

  3. Prise en charge des intoxications par envenimation chez les enfants ...

    African Journals Online (AJOL)

    Les signes cliniques étaient dominés par les douleurs (98%) suivies des hémorragies (73,80%) pour les morsures de serpent, la douleur pour les piqures de scorpion (100%) et les oedèmes pour les piqures d'abeilles (100%). Les cas de morsure de serpent ont été traités par le sérum antivenimeux polyvalent. Conclusion: ...

  4. Lumbar vertebral body and pars fractures following laminectomy

    OpenAIRE

    Yadhati, Akshay; Kurra, Swamy; Tallarico, Richard A.; Lavelle, William F.

    2017-01-01

    Abstract A 56-year-old alcoholic male incurred L5 vertebral body and bilateral L4 pars fractures with progressive L4 on L5 anterolisthesis following low-energy falls while intoxicated. Recently, he had a L3?S1 laminectomy for lumbar spinal stenosis with claudication. Preoperative imaging and radiographs were negative for pars defects and instability, so an isolated decompressive surgery was performed. Following low-energy falls, his outpatient work-up revealed fractures through the bilateral ...

  5. Analysis of ParB-centromere interactions by multiplex SPR imaging reveals specific patterns for binding ParB in six centromeres of Burkholderiales chromosomes and plasmids.

    Directory of Open Access Journals (Sweden)

    Flavien Pillet

    Full Text Available Bacterial centromeres-also called parS, are cis-acting DNA sequences which, together with the proteins ParA and ParB, are involved in the segregation of chromosomes and plasmids. The specific binding of ParB to parS nucleates the assembly of a large ParB/DNA complex from which ParA-the motor protein, segregates the sister replicons. Closely related families of partition systems, called Bsr, were identified on the chromosomes and large plasmids of the multi-chromosomal bacterium Burkholderia cenocepacia and other species from the order Burkholeriales. The centromeres of the Bsr partition families are 16 bp palindromes, displaying similar base compositions, notably a central CG dinucleotide. Despite centromeres bind the cognate ParB with a narrow specificity, weak ParB-parS non cognate interactions were nevertheless detected between few Bsr partition systems of replicons not belonging to the same genome. These observations suggested that Bsr partition systems could have a common ancestry but that evolution mostly erased the possibilities of cross-reactions between them, in particular to prevent replicon incompatibility. To detect novel similarities between Bsr partition systems, we have analyzed the binding of six Bsr parS sequences and a wide collection of modified derivatives, to their cognate ParB. The study was carried out by Surface Plasmon Resonance imaging (SPRi mulitplex analysis enabling a systematic survey of each nucleotide position within the centromere. We found that in each parS some positions could be changed while maintaining binding to ParB. Each centromere displays its own pattern of changes, but some positions are shared more or less widely. In addition from these changes we could speculate evolutionary links between these centromeres.

  6. Analysis of ParB-centromere interactions by multiplex SPR imaging reveals specific patterns for binding ParB in six centromeres of Burkholderiales chromosomes and plasmids

    Science.gov (United States)

    Pillet, Flavien; Passot, Fanny Marie

    2017-01-01

    Bacterial centromeres–also called parS, are cis-acting DNA sequences which, together with the proteins ParA and ParB, are involved in the segregation of chromosomes and plasmids. The specific binding of ParB to parS nucleates the assembly of a large ParB/DNA complex from which ParA—the motor protein, segregates the sister replicons. Closely related families of partition systems, called Bsr, were identified on the chromosomes and large plasmids of the multi-chromosomal bacterium Burkholderia cenocepacia and other species from the order Burkholeriales. The centromeres of the Bsr partition families are 16 bp palindromes, displaying similar base compositions, notably a central CG dinucleotide. Despite centromeres bind the cognate ParB with a narrow specificity, weak ParB-parS non cognate interactions were nevertheless detected between few Bsr partition systems of replicons not belonging to the same genome. These observations suggested that Bsr partition systems could have a common ancestry but that evolution mostly erased the possibilities of cross-reactions between them, in particular to prevent replicon incompatibility. To detect novel similarities between Bsr partition systems, we have analyzed the binding of six Bsr parS sequences and a wide collection of modified derivatives, to their cognate ParB. The study was carried out by Surface Plasmon Resonance imaging (SPRi) mulitplex analysis enabling a systematic survey of each nucleotide position within the centromere. We found that in each parS some positions could be changed while maintaining binding to ParB. Each centromere displays its own pattern of changes, but some positions are shared more or less widely. In addition from these changes we could speculate evolutionary links between these centromeres. PMID:28562673

  7. The urokinase receptor (uPAR) and the uPAR-associated protein (uPARAP/Endo180)

    DEFF Research Database (Denmark)

    Behrendt, Niels

    2004-01-01

    The breakdown of the barriers formed by extracellular matrix proteins is a pre-requisite for all processes of tissue remodeling. Matrix degradation reactions take part in specific physiological events in the healthy organism but also represent a crucial step in cancer invasion. These degradation...... processes involve a highly organized interplay between proteases and their cellular binding sites as well as specific substrates and internalization receptors. This review article is focused on two components, the urokinase plasminogen activator receptor (uPAR) and the uPAR-associated protein (uPARAP, also...... designated Endo180), that are considered crucially engaged in matrix degradation. uPAR and uPARAP have highly diverse functions, but on certain cell types they interact with each other in a process that is still incompletely understood. uPAR is a glycosyl-phosphatidylinositol-anchored glycoprotein...

  8. Une approche de la conservation in situ par l’étude d’un système semencier informel : cas du cocotier au Vanuatu (Pacifique Sud

    Directory of Open Access Journals (Sweden)

    Labouisse Jean-Pierre

    2001-09-01

    Full Text Available L’amélioration génétique du cocotier (Cocos nucifera L. a été, jusqu’à présent, centrée sur l’augmentation de la productivité, exprimée en coprah par hectare, et accessoirement sur la recherche de résistance aux maladies. La voie de l’hybridation s’est révélée particulièrement efficace pour améliorer le rendement en coprah ainsi que la précocité de mise à fruit par le croisement d’écotypes Nains et Grands [1]. Cependant, d’autres caractéristiques comme la qualité gustative, l’aptitude à la transformation de la noix et l’utilisation des autres composantes de l’arbre ont été peu étudiées et n’ont pas fait l’objet de programmes d’amélioration élaborés comme pour le rendement en coprah. Or, les villageois des zones tropicales utilisent quotidiennement les différentes parties du cocotier pour, entre autres, la confection d’objets domestiques et artisanaux, de matériaux pour l’habitat ou pour la pharmacopée. Aussi observe-t-on, dans nombre de pays, une préférence marquée des communautés rurales pour les écotypes Grands locaux présentant un haut niveau de variabilité intrapopulation et fournissant une grande variété de produits sur une longue période avec un minimum de travail et d’intrants [2]. La volatilité et le faible niveau actuel des prix du coprah et de l’huile de coco sur le marché mondial renforcent l’idée d’une nécessaire diversification des produits du cocotier, notamment pour nombre de pays insulaires du Pacifique où le coprah constitue encore une part importante des ressources d’exportation et une des principales sources de revenus monétaires des populations rurales. Ces pays souffrent, en outre, d’un manque structurel de compétitivité dû à l’éloignement des grands centres de consommation internationaux, à la dispersion de la production de coprah dans les archipels et au coût relativement élevé du travail [3].

  9. Attenuated vasodilator effectiveness of protease-activated receptor 2 agonist in heterozygous par2 knockout mice.

    Directory of Open Access Journals (Sweden)

    John C Hennessey

    Full Text Available Studies of homozygous PAR2 gene knockout mice have described a mix of phenotypic effects in vitro and in vivo. However, there have been few studies of PAR2 heterozygous (wild-type/knockout; PAR2-HET mice. The phenotypes of many hemi and heterozygous transgenic mice have been described as intermediates between those of wild-type and knockout animals. In our study we aimed to determine the effects of intermediary par2 gene zygosity on vascular tissue responses to PAR2 activation. Specifically, we compared the vasodilator effectiveness of the PAR2 activating peptide 2-furoyl-LIGRLO-amide in aortas of wild-type PAR2 homozygous (PAR2-WT and PAR2-HET mice. In myographs under isometric tension conditions, isolated aortic rings were contracted by alpha 1-adrenoeceptor agonist (phenylephrine, and thromboxane receptor agonist (U46619 and then relaxation responses by the additions of 2-furoyl-LIGRLO-amide, acetylcholine, and nitroprusside were recorded. A Schild regression analysis of the inhibition by a PAR2 antagonist (GB-83 of PAR2 agonist-induced aortic ring relaxations was used to compare receptor expression in PAR2-WT to PAR2-HET. PAR2 mRNA in aortas was measured by quantitative real-time PCR. In aortas contracted by either phenylephrine or U46619, the maximum relaxations induced by 2-furoyl-LIGRLO-amide were less in PAR2-HET than in the gender-matched PAR2-WT. GB-83 was 3- to 4-fold more potent for inhibition of 2fly in PAR2-HET than in PAR2-WT. PAR2 mRNA content of aortas from PAR2-HET was not significantly different than in PAR2-WT. Acetylcholine- and nitroprusside-induced relaxations of aortas from PAR2-HET were not significantly different than in PAR2-WT and PAR2 knockout. An interesting secondary finding was that relaxations induced by agonists of PAR2 and muscarinic receptors were larger in females than in males. We conclude that the lower PAR2-mediated responses in PAR2-HET aortas are consistent with evidence of a lower quantity of functional

  10. Contribution of the tomography by positrons emission with fluorodeoxyglucose labelled with fluorine 18 in the case of a Takayasu disease; Apport de la tomographie par emission de positons au fluorodeoxyglucose marque au fluor 18 dans un cas de maladie de Takayasu

    Energy Technology Data Exchange (ETDEWEB)

    Cristofol, H.; Esquerre, J.P.; Payoux, P. [Service de medecine nucleaire, CHU Purpan, Toulouse, (France); Delavigne, K. [service de medecine interne, CHU Purpan, Toulouse, (France)

    2009-05-15

    The objective of the clinical case is to evaluate the interest of the PET with {sup 18}F.D.G. to confirm the active character and the disease extent in a suspicion of Takayasu disease. Conclusions: the PET with {sup 18}F.D.G. is a non invasive and low irradiating mean of metabolic imaging to evaluate the activity and the extent of arteritis of big and middle caliber vessels as the Takayasu disease and in an early way. To do again a PET some months after a cortico-therapy would allow to have an idea of the prognosis in function of the still active or not character. (N.C.)

  11. Plutonium behavior during the early diagenesis of marine sediments: applications to two marine environments labelled by radionuclides released from reprocessing plants; Etude du comportement du plutonium au cours de la diagenese precoce des sediments marins: applications a deux environnements marins marques par les rejets issus d'usines de retraitement de combustibles uses

    Energy Technology Data Exchange (ETDEWEB)

    Gouzy, A

    2004-12-15

    The plutonium released into the English Channel and the Irish Sea by nuclear fuel reprocessing plants is mainly associated to sediments. Nevertheless, this association is partially reversible. This work combines a field study, carried out on the Cumbrian mud patch and the Esk estuary (Eastern Irish Sea), and laboratory experiments performed on carbonaceous coarse-grained sediments collected in the Central Channel. It presents new data on the plutonium solid partition in sediments and suggests realistic scenarios for describing its release from sediments to the water column. The role of reactive sulphides acting as temporary sink phases is shown in anoxic sediments; those sulphides are liable to release dissolved plutonium upon their oxidation. The plutonium is also bound to carbonates within the carbonaceous matrix and as carbonate surface complexes. Conceptual schemes of the behaviour of the plutonium in marine sediments are proposed; they highlight the strong remobilization potential of plutonium from marine sediments to the interstitial water. Its plutonium content can be injected into the overlying water column. (author)

  12. Serum suPAR in patients with FSGS: trash or treasure?

    NARCIS (Netherlands)

    Maas, R.J.H.; Deegens, J.K.J.; Wetzels, J.F.M.

    2013-01-01

    The urokinase-type plasminogen activator receptor (uPAR) has important functions in cell migration. uPAR can be shed from the cell membrane resulting in soluble uPAR (suPAR). Further cleavage gives rise to shorter fragments with largely unknown functions. Recent studies have demonstrated that both

  13. Combined pars plana vitrectomy-scleral buckle versus pars plana vitrectomy for proliferative vitreoretinopathy.

    Science.gov (United States)

    Lai, Frank H P; Lo, Ernie C F; Chan, Vesta C K; Brelen, Mårten; Lo, Wai Ling; Young, Alvin L

    2016-04-01

    The purpose of the study is to evaluate the surgical outcomes of combined pars plana vitrectomy-scleral buckle (PPV-SB) versus pars plana vitrectomy (PPV) for rhegmatogenous retinal detachment complicated with proliferative vitreoretinopathy (PVR). One thousand one hundred and seventy four patients with rhegmatogenous retinal detachment surgery between January 2002 and December 2013 were retrospectively reviewed. Patients with grade C PVR treated with either combined PPV-SB or PPV alone were included in the study. Study outcomes included single surgery anatomic success rate and postoperative visual outcome at 12 months postoperatively. Seventy-seven patients with grade C PVR were identified for analysis. At the end of 12-month follow-up, 80.5 % eyes (33/41) in the PPV-SB group and 58.3 % eyes (21/36) in the PPV group achieved single surgery anatomical success. In a multiple logistic regression model, none of the baseline variables (age, gender, macula status, grade of PVR, extent of detachment, presence of vitreous hemorrhage, lens status, status of high myopia) nor types of retinal detachment surgery (use of scleral buckle, barrier endolaser, 360 degree endolaser, cryopexy, retinectomy, tamponade agent, phacoemulsification) had significant effect on single surgery anatomical success. The post-treatment mean logMAR visual acuity of the PPV-SB group was 1.58 ± 0.58 and the PPV group was 1.57 ± 0.61. There was no significant difference in the postoperative visual acuity between the two groups (P = 0.849). For patients with grade C PVR, PPV-SB did not demonstrate a superiority over PPV alone in achieving single surgery anatomical success.

  14. Pars planitis: Epidemiology, clinical characteristics, management and visual prognosis

    Directory of Open Access Journals (Sweden)

    Pinar Cakar Ozdal

    2015-01-01

    Full Text Available Pars planitis is an idiopathic chronic intermediate uveitis which predominantly affects children and adolescents, and accounts for 5-26.7% of pediatric uveitis. Although an autoimmune process with a genetic predisposition has been suggested, its etiology still remains unknown. The most common presenting symptoms are floaters and blurred vision. Diffuse vitreous cells, haze, snowballs and snowbanks are typical findings of pars planitis. Peripheral retinal vasculitis, optic disc edema and anterior segment inflammation are other well-known findings. Although pars planitis is known to be a benign form of uveitis in most cases, it may become a potentially blinding disease due to complications including cataract, cystoid macular edema, vitreous opacities and optic disc edema. Cystoid macular edema is the most common cause of visual morbidity. Band keratopathy, epiretinal membrane formation, vitreous condensation, neovascularizations, vitreous hemorrhage, retinal detachment, cyclitic membranes, glaucoma and amblyopia may develop as a consequence of the chronic course of the disease. Exclusion of infectious and non-infectious causes which may present with intermediate uveitis is of utmost importance before starting treatment. Treatment of pars planitis has been a controversial issue. There is no consensus specifically for treatment of cases with minimal inflammation and relatively good visual acuity. However, current experience shows that pars planitis may cause severe inflammation and needs an aggressive treatment. A stepladder approach including corticosteroids, immunosupressive agents, anti-tumor necrosis factor-alpha and pars plana vitrectomy and/or laser photocoagulation is the most commonly used method for treatment of pars planitis. Adequate control of inflammation and prompt detection of associated complications are crucial in order to improve the overall prognosis of the disease.

  15. Par Pond vegetation status Summer 1995 -- June survey descriptive summary

    Energy Technology Data Exchange (ETDEWEB)

    Mackey, H.E. Jr.; Riley, R.S.

    1995-06-01

    The water level of Par Pond was lowered approximately 20 feet in mid-1991 in order to protect downstream residents from possible dam failure suggested by subsidence on the downstream slope of the dam and to repair the dam. This lowering exposed both emergent and nonemergent macrophyte beds to drying conditions resulting in extensive losses. A survey of the shoreline aquatic plant communities in June 1995, three months after the refilling of Par Pond to approximately 200 feet above mean sea level, indicated that much of the original plant communities and the intermediate shoreline communities present on the exposed sediments have been lost. The extensive old-field and emergent marsh communities that were present on the exposed shoreline during the drawdown have been flooded and much of the pre-drawdown Par Pond aquatic plant communities have not had sufficient time for re-establishment. The shoreline does, however, have extensive beds of maidencane which extend from the shoreline margin to areas as deep as 2 and perhaps 3 meters. Scattered individual plants of lotus and watershield are common and may indicate likely directions of future wetland development in Par Pond. In addition, within isolated coves, which apparently received ground water seepage and/or stream surface flows during the period of the Par Pond draw down, extensive beds of waterlilies and spike rush are common. Invasion of willow and red maple occurred along the lake shoreline as well. Although not absent from this survey, evidence of the extensive redevelopment of the large cattail and eel grass beds was not observed in this first survey of Par Pond. Future surveys during the growing seasons of 1995, 1996, and 1997 along with the evaluation of satellite date to map the areal extent of the macrophyte beds of Par Pond are planned.

  16. Pars Planitis: Epidemiology, Clinical Characteristics, Management and Visual Prognosis.

    Science.gov (United States)

    Ozdal, Pinar Cakar; Berker, Nilufer; Tugal-Tutkun, Ilknur

    2015-01-01

    Pars planitis is an idiopathic chronic intermediate uveitis which predominantly affects children and adolescents, and accounts for 5-26.7% of pediatric uveitis. Although an autoimmune process with a genetic predisposition has been suggested, its etiology still remains unknown. The most common presenting symptoms are floaters and blurred vision. Diffuse vitreous cells, haze, snowballs and snowbanks are typical findings of pars planitis. Peripheral retinal vasculitis, optic disc edema and anterior segment inflammation are other well-known findings. Although pars planitis is known to be a benign form of uveitis in most cases, it may become a potentially blinding disease due to complications including cataract, cystoid macular edema, vitreous opacities and optic disc edema. Cystoid macular edema is the most common cause of visual morbidity. Band keratopathy, epiretinal membrane formation, vitreous condensation, neovascularizations, vitreous hemorrhage, retinal detachment, cyclitic membranes, glaucoma and amblyopia may develop as a consequence of the chronic course of the disease. Exclusion of infectious and non-infectious causes which may present with intermediate uveitis is of utmost importance before starting treatment. Treatment of pars planitis has been a controversial issue. There is no consensus specifically for treatment of cases with minimal inflammation and relatively good visual acuity. However, current experience shows that pars planitis may cause severe inflammation and needs an aggressive treatment. A stepladder approach including corticosteroids, immunosupressive agents, anti-tumor necrosis factor-alpha and pars plana vitrectomy and/or laser photocoagulation is the most commonly used method for treatment of pars planitis. Adequate control of inflammation and prompt detection of associated complications are crucial in order to improve the overall prognosis of the disease.

  17. PH motifs in PAR1&2 endow breast cancer growth

    OpenAIRE

    Kancharla, A.; Maoz, M.; Jaber, M.; Agranovich, D.; Peretz, T.; Grisaru-Granovsky, S.; Uziely, B.; Bar-Shavit, R.

    2015-01-01

    Although emerging roles of protease-activated receptor1&2 (PAR1&2) in cancer are recognized, their underlying signalling events are poorly understood. Here we show signal-binding motifs in PAR1&2 that are critical for breast cancer growth. This occurs via the association of the pleckstrin homology (PH) domain with Akt/PKB as a key signalling event of PARs. Other PH-domain signal-proteins such as Etk/Bmx and Vav3 also associate with PAR1 and PAR2 through their PH domains. PAR1 and PAR2 bind wi...

  18. Par Pond vegetation status Summer 1995 -- October survey descriptive summary

    Energy Technology Data Exchange (ETDEWEB)

    Mackey, H.E. Jr.; Riley, R.S.

    1995-11-01

    The water level of Par Pond was lowered approximately 20 feet in mid-1991 in order to protect downstream residents from possible dam failure suggested by subsidence on the downstream slope of the dam and to repair the dam. This lowering exposed both emergent and nonemergent macrophyte beds to drying conditions resulting in extensive losses. A survey of the emergent shoreline aquatic plant communities began in June 1995, three months after the refilling of Par Pond to approximately 200 feet above mean sea level and continued with this late October survey. Communities similar to the pre-drawdown Par Pond aquatic plant communities are becoming re-established; especially, beds of maiden cane, lotus, waterlily, and watershield are now extensive and well established. Cattail occurrence continues to increase, but large beds common to Par Pond prior to the drawdown have not formed. Future surveys throughout 1996 and 1997, along with the continued evaluation of satellite data to map the areal extent of the macrophyte beds of Par Pond, are planned.

  19. Plasma suPAR is lowered by smoking cessation

    DEFF Research Database (Denmark)

    Eugen-Olsen, Jesper; Ladelund, Steen; Sørensen, Lars Tue

    2016-01-01

    , in smokers, predictive of long-term lung cancer development. Whether smoking cessation impacts the suPAR level is unknown. MATERIALS AND METHODS: Forty-eight smokers were randomized into three groups of 16: (i) continued to smoke 20 cigarettes per day, (ii) refrained from smoking and used transdermal...... nicotine patches and (iii) refrained from smoking and used placebo patches. Nonsmokers were included for comparison. suPAR and C-reactive protein (CRP) levels were measured by ELISA. RESULTS: At baseline, the suPAR level was significantly higher in the 48 smokers (median 3·2 ng mL, IQR (2·5-3·9)) than...... in 46 never smokers (1·9 ng/mL (1·7-2·2)). In smokers randomized to smoking cessation, suPAR levels after 4 weeks of stopping were decreased and no longer significantly different from the never smokers values. SuPAR decreased in both those who received a placebo as well as nicotine patch. Interestingly...

  20. CFD Application to Hydrogen Risk Analysis and PAR Qualification

    Directory of Open Access Journals (Sweden)

    Jinbiao Xiong

    2009-01-01

    Full Text Available A three dimensional computation fluid dynamics (CFD code, GASFLOW, is applied to analyze the hydrogen risk for Qinshan-II nuclear power plant (NPP. In this paper, the effect of spray modes on hydrogen risk in the containment during a large break loss of coolant accident (LBLOCA is analyzed by selecting three different spray strategies, that is, without spray, with direct spray and with both direct and recirculation spray. A strong effect of spray modes on hydrogen distribution is observed. However, the efficiency of the passive auto-catalytic recombiners (PAR is not substantially affected by spray modes. The hydrogen risk is significantly increased by the direct spray, while the recirculation spray has minor effect on it. In order to simulate more precisely the processes involved in the PAR operation, a new PAR model is developed using CFD approach. The validation shows that the results obtained by the model agree well with the experimental results.

  1. PARS PLANA VITRECTOMY THROUGH A CUSTOM FLEXIBLE IRIS PROSTHESIS.

    Science.gov (United States)

    Toygar, Okan; Snyder, Michael E; Riemann, Christopher D

    2016-08-01

    To demonstrate the outcomes of pars plana vitrectomy in patients with custom flexible iris prosthesis (CFIP). The medical records of patients who underwent CFIP placement were retrospectively reviewed. Patients who underwent pars plana vitrectomy concurrent with or after placement of CFIP were identified. Preoperative, intraoperative, and postoperative parameters were analyzed. Surgeons were surveyed regarding the technical aspects of the vitreoretinal surgery. Outcome measures included: anatomic success, complications, surgeon-rated ease of visualization through CFIP during pars plana vitrectomy, and Snellen best-corrected visual acuity. Twenty-four surgeries were performed in 20 eyes of 20 patients. Postoperative best-corrected visual acuity improved in 11 eyes (55%), remained unchanged in 5 eyes (25%), and declined in 4 eyes (20%) after a mean follow-up of 16.1 ± 16.\

  2. Protease activated receptor-2 (PAR2): possible target of phytochemicals.

    Science.gov (United States)

    Kakarala, Kavita Kumari; Jamil, Kaiser

    2015-09-01

    The use of phytochemicals either singly or in combination with other anticancer drugs comes with an advantage of less toxicity and minimal side effects. Signaling pathways play central role in cell cycle, cell growth, metabolism, etc. Thus, the identification of phytochemicals with promising antagonistic effect on the receptor/s playing key role in single transduction may have better therapeutic application. With this background, phytochemicals were screened against protease-activated receptor 2 (PAR2). PAR2 belongs to the superfamily of GPCRs and is an important target for breast cancer. Using in silico methods, this study was able to identify the phytochemicals with promising binding affinity suggesting their therapeutic potential in the treatment of breast cancer. The findings from this study acquires importance as the information on the possible agonists and antagonists of PAR2 is limited due its unique mechanism of activation.

  3. La projection par plasma : une revue

    Science.gov (United States)

    Fauchais, P.; Grimaud, A.; Vardelle, A.; Vardelle, M.

    are presented in the fields of aeronautics and mechanics. La qualité d'un dépôt projete par plasma dépend de nombreux paramètres que l'on commence à mieux appréhender du fait des progrès de la modélisation et de la métrologie tant des écoulements plasmas que des transferts plasma-particules ou que des conditions d'écrasement et de refroidissement des particules lors de leur impact sur le substrat ou les couches déjà déposées. Les techniques de mesure utilisdes et leurs limitations sont d'abord rappelées tant pour les jets de plasma que pour les particules en vol et l'importance des différents phénomènes intervenant dans les transferts plasma-particules est soulignée : gradients de température et de concentration d'espèces chimiques très élevés autour des particules, effets de propagation de la chaleur, notamment pour les particules céramiques, effet d'évaporation, effet de raréfaction sensible dès la pression atmosphérique. Les problèmes de distribution de taille et de vitesse d'injection des particules sont également abordés car ils conditionnent les distributions de trajectoires et donc le traitement des particules dans le jet de plasma. La génération du plasma montre d'une part 1'influence considérable de l'injection du gaz, de sa nature, du dessin de la chambre d'arc et de la tuyère ainsi que du pompage de l'air ambiant sur la longueur des jets de plasma d'arc et d'autre part les problèmes d'injection pour éviter le couplage avec la décharge dans les jets de plasmas R.E Tout ceci est illustré avec des exemples de dépôt d'alumine, de zircone, de cermet carbure et de nickel. L'écrasement des particules est ensuite abordé avec les problèmes de réactions chimiques, de trempe ultra-rapide et donc de structure cristalline des dépôts, d'adhdsion mais aussi de containtes résiduelles et de leur contrôle via les gradients de température dans les dépôts pendant le tir. Enfin quelques applications actuelles sont pr

  4. Contributions of Protease-Activated Receptors PAR1 and PAR4 to Thrombin-Induced GPIIbIIIa Activation in Human Platelets.

    Science.gov (United States)

    Duvernay, Matthew T; Temple, Kayla J; Maeng, Jae G; Blobaum, Anna L; Stauffer, Shaun R; Lindsley, Craig W; Hamm, Heidi E

    2017-01-01

    Human platelets display a unique dual receptor system for responding to its primary endogenous activator, α-thrombin. Because of the lack of efficacious antagonists, the field has relied on synthetic peptides and pepducins to describe protease-activated receptor PAR1 and PAR4 signaling. The precise contributions of each receptor have not been established in the context of thrombin. We took advantage of newly discovered PAR antagonists to contrast the contribution of PAR1 and PAR4 to thrombin-mediated activation of the platelet fibrin receptor (GPIIbIIIa). PAR1 is required for platelet activation at low but not high concentrations of thrombin, and maximal platelet activation at high concentrations of thrombin requires PAR4. As the concentration of thrombin is increased, PAR1 signaling is quickly overcome by PAR4 signaling, leaving a narrow window of low thrombin concentrations that exclusively engage PAR1. PAR4 antagonism reduces the maximum thrombin response by over 50%. Thus, although the PAR1 response still active at higher concentrations of thrombin, this response is superseded by PAR4. Truncation of a known PAR4 antagonist and identification of the minimum pharmacophore converted the mechanism of inhibition from noncompetitive to competitive, such that the antagonist could be outcompeted by increasing doses of the ligand. Fragments retained efficacy against both soluble and tethered ligands with lower cLogP values and an increased free fraction in plasma. These reversible, competitive compounds represent a route toward potentially safer PAR4 antagonists for clinical utility and the development of tools such as radioligands and positron emission tomography tracers that are not currently available to the field for this target. Copyright © 2016 by The American Society for Pharmacology and Experimental Therapeutics.

  5. La parálisis cerebral en el cine

    OpenAIRE

    María Lucila MERINO MARCOS

    2005-01-01

    [ES] En los últimos veinticinco años se han rodado un número importante de películas en las que el protagonista o algún personaje secundario presentaban o simulaban una parálisis cerebral. Estos personajes en ocasiones han sido interpretados por personas con minusvalías. En la narración de su historia las cintas han incluido diversos aspectos sanitario-asistenciales, sociales y familiares obligados en el campo de la parálisis cerebral. [EN] In the last twenty-five years, a number of import...

  6. Transplantaciones tendinosas por parálisis radial

    OpenAIRE

    De la Puente, Luis; Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú

    2014-01-01

    Entre las parálisis periféricas determinadas por lesiones traumáticas de los nervios, la parálisis del radial es la que origina mayores perturbaciones en las funciones del miembro. La inhabilitación del miembro enfermo es, generalmente, mas seria que la que ocasionan las lesiones del nervio cubital o del mediano, nervios que, funcionalmente, son susceptibles de suplirse en el territorio muscular que inervan conjuntamente. Among peripheral paralysis determined by traumatic nerve injuries, p...

  7. Predicting Spectral and PAR Light Attenuation in Greenlandic Coastal Waters

    DEFF Research Database (Denmark)

    Murray, Ciarán; Stedmon, Colin A.; Markager, Stiig

    present models for spectral and PAR (photosynthetically active radiation) attenuation in two contrasting Greenlandic fjords, Godthåbsfjord (SW Greenland) and Young Sound (NE Greenland). The fjords differ in the character and concentrations of optically active components present: dissolved organic material...... (CDOM), phytoplankton pigments and inorganic particles. These differences are due in part to hydrography and to the sources of meltwater: respectively, fjord-terminating and land-terminating glaciers. We present a model to explain the variation in spectral and PAR irradiance in terms of the variation...

  8. Predicting spectral and PAR light attenuation in Greenlandic coastal waters

    DEFF Research Database (Denmark)

    Murray, Ciarán; Markager, Stiig; Stedmon, Colin

    present models for spectral and PAR (photosynthetically active radiation) attenuation in two contrasting Greenlandic fjords, Godthåbsfjord (SW Greenland) and Young Sound (NE Greenland). The fjords differ in the character and concentrations of optically active components present: dissolved organic material...... (CDOM), phytoplankton pigments and inorganic particles. These differences are due in part to hydrography and to the sources of meltwater: respectively, fjord-terminating and land-terminating glaciers. We present a model to explain the variation in spectral and PAR irradiance in terms of the variation...

  9. Intoxication par la paraphényléne-diamine

    African Journals Online (AJOL)

    raoul

    9 mars 2011 ... La symptomatologie des intoxications aigues par la PPD est stéréotypée, le tableau clinique était dominé au départ par un oedème cervico-facial avec une ... réalisée chez 13% des patients devant une hyperkaliémie (8,6%) et/ou effondrement de la diurèse (5%), en moyenne 2 séances avec des extrêmes ...

  10. Intact and cleaved uPAR forms: diagnostic and prognostic value in cancer

    DEFF Research Database (Denmark)

    Rasch, M.G.; Lund, I.K.; Hoyer-Hansen, G.

    2008-01-01

    PA-catalyzed cleavage of uPAR is fast on the cell surface, when uPA is bound to a neighboring uPAR molecule. uPAR can be shed from the cell surface. However, the soluble form cannot be cleaved by uPA. Glycolipid-anchored and soluble forms of intact, uPAR(I-III), and cleaved receptor, uPAR(II-III) and uPAR(I), have been...... identified in tissue and body fluids. It is well-established, that the total amount of all uPAR forms is a strong prognostic marker in different types of cancer. Using immunoassays, measuring the individual uPAR forms, has revealed that the cleaved uPAR forms are even stronger prognostic markers and have...

  11. [A middle to long-term clinical study on pars flaccida and pars tensa cholesteatoma using survival analysis].

    Science.gov (United States)

    Somekawa, Yukihiro; Nagashima, Tsutomu; Masaki, Tomoyuki; Asano, Katsuji; Yajima, Ryoto; Himi, Tetsuo

    2014-12-01

    The current clinical study was performed on 311 cases of pars flaccida and 89 cases of pars tensa cholesteatoma which were treated with canal wall reconstructed tympanoplasty between 1991 and 2012. The average follow-up time of these patients was 5.3 years. Since follow-up periods were different in each case and some censored patients were involved, we used survival analysis on this study to discuss the cumulative rates of disease-free successful cases and the rates of recurrent cholesteatoma throughout the postoperative course. The disease-free successful cases were defined as those cases in which patients were both out of re-operation with recurrent and residual cholesteatoma and out of revision operation with another problem, furthermore, maintained good hearing outcome. Based on the criteria set by the Japan Otological Society (2010), the cases that satisfied the following were evaluated as good hearing results; (a) a successful case in which preoperative bone conduction was used, and (b) a case in which the postoperative air-bone gap was within 20dB after tympanoplasty for chronic otitis media. The analysis results were shown for each of (a) and (b). 1. In pars flaccida cholesteatoma, the 5-year survival rate of successful case was (a) 76.1% and (b) 83.9%, the 10-year survival rate was (a) 58.9% and (b) 73.0%. In pars tensa cholesteatoma, the 5-year survival rate of successful cases was (a) 57.7% and (b) 63.5%, the 10-year rate was (a) 42.1% and (b) 56.9%. A significant difference was seen between pars flaccida and pars tensa cholesteatoma (p pars flaccida cholesteatoma, the 5-year recurrence rate was 7.6% and the 10-year rate was 15.3%, and the recurrence rate increased gradually throughout the follow-up period. On the other hand, in pars tensa cholesteatoma, the increase in the recurrence rate reached a peak 15.8% at 5.5 years after the surgery. A long-term follow-up is necessary when evaluating the clinical results after tympanoplasty.

  12. A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay to quantify Parietaria judaica major allergens, Par j 1 and Par j 2.

    Science.gov (United States)

    Arilla, M C; González-Rioja, R; Ibarrola, I; Mir, A; Monteseirín, J; Conde, J; Martínez, A; Asturias, J A

    2006-01-01

    Parietaria pollen is one of the most important causes of pollinosis in Mediterranean countries. Parietaria judaica pollen extract presents two major allergens, Par j 1 and Par j 2, that belong to the lipid transfer protein family. To develop an ELISA for quantification of both major allergens of P. judaica pollen extracts, and to assert correlation of these allergens content with the allergenic activity of extracts. Natural Par j 1-Par j 2 allergens were purified by gel filtration, ion exchange, and affinity chromatography and identified by mass spectrometry. Rabbit antisera were obtained using this protein preparation as antigen and used for immunoaffinity purification of nPar j 1-Par j 2. BALB/c mice were immunized with the immunopurified nPar j 1-Par j 2 and after fusion and screening by direct ELISA, 5D4 monoclonal antibody was selected as capture antibody to develop a quantitative two-site ELISA. Bound proteins were detected by a biotinylated Par j 1-Par j 2-specific polyclonal antibody. The optimized ELISA was developed from 25 to 8000 pg/mL of purified Par j 1-Par j 2, and a linear portion of 200-1000 pg/mL. The intraassay and interassay coefficients of variation were lower than 7% and 14% respectively. The assay was very sensitive and specific as it had a detection limit of 25 pg/mL and did not detect reactivity with the same family plants, as Urtica. Par j 1-Par j 2 allergens content was measured in 14 P. judaica and two P. officinalis pollen extracts showing a significant correlation with their allergenic activity measured by enzyme allergosorbent test inhibition. The results proved the usefulness of the two-sandwich ELISA for the standardization of Parietaria pollen extracts intended for clinical use, because of its good correlation with allergenic potency.

  13. La lecture de Merleau-Ponty par Renaud Barbaras

    Czech Academy of Sciences Publication Activity Database

    Pechar, Jiří

    2015-01-01

    Roč. 11, č. 2 (2015), s. 1-6 ISSN 1336-6556 Institutional support: RVO:67985955 Keywords : Merleau-Ponty * Husserl * phenomenology * linguistics of Ferdinand de Saussure Subject RIV: AA - Philosophy ; Religion http://www.ostium.sk/sk/la-lecture-de-merleau-ponty-par-renaud-barbaras/

  14. Radiative transfer code: Application to the calculation of PAR

    Indian Academy of Sciences (India)

    Radiative transfer code: Application to the calculation of PAR. DEVRED EMMANUEL, DUBUISSON .... water vapor from Leckner (1978), with absorption coefficients for these gases taken from Gregg and. Carder (1990). ... law for the size distribution. The particle size ranges from 0.01 "m to 50 "m. 3. Validation of the code.

  15. Indications and Visual Outcome of First Hundred Pars Plana ...

    African Journals Online (AJOL)

    tulyasys

    Indications and Visual Outcome of First. Hundred Pars Plana Vitrectomies at Makkah. Specialist Eye Hospital, Kano, Nigeria. Abdulsalam Shuaib, Abdul Fattah Memon1. Department of Ophthalmology, Bayero University Kano/Aminu Kano Teaching Hospital, Kano State, Nigeria, 1Vitreoretinal Unit,. Al Ibrahim Eye Hospital, ...

  16. Indications and visual outcome of first hundred pars plana ...

    African Journals Online (AJOL)

    Conclusion: Rhegmatogenous retinal detachment and vitreous hemorrhage are the major indications for pars plana vitrectomy in this environment. As vitreoretinal disorders have become more common in this part of the world, there would be a corresponding increase in demand for vitreoretinal services. There is therefore a ...

  17. Sir Mokshagundam Visvesvaraya–A Visionary Engineer par ...

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 7; Issue 5. Sir Mokshagundam Visvesvaraya – A Visionary Engineer par Excellence. A V Shankara Rao. Article-in-a-Box Volume 7 Issue 5 May 2002 pp 3-5. Fulltext. Click here to view fulltext PDF. Permanent link:

  18. Efficacite et tolerance du captopril par voie sublinguale dans la ...

    African Journals Online (AJOL)

    insuffisance rénale chronique étaient les deux principales causes d'échec thérapeutique. Conclusion : Le captopril par voie sublinguale est donc aussi efficace chez le noir africain que chez le caucasien. Dans le contexte de pays en voie de ...

  19. Traitement De La Peripneumonie Contagieuse Bovine Par L ...

    African Journals Online (AJOL)

    oxytétracycline LA) dans le traitement des bovins atteints de péripneumonie contagieuse bovine (PPCB) et de déterminer le risque de transmission de la maladie à partir d'animaux traités. Une transmission expérimentale a été conduite par la mise ...

  20. Parasitisme des champs semenciers de cacaoyers par les ...

    African Journals Online (AJOL)

    SARAH

    30 janv. 2015 ... Parasitisme des champs semenciers de cacaoyers par les Loranthaceae dans la localité de Nkoemvone (sud. Cameroun). ONDOUA Joseph Marie1, DIBONG Siegfried Didier (2,3,4)*, TAFFOUO Victor Désiré (2,4), NGOTTA BIYON. Jacques Bruno (2). (1). Département de Botanique et de Physiologie ...

  1. Parasitisme de Dacryodes edulis par le genre Tapinanthus ...

    African Journals Online (AJOL)

    SARAH

    31 août 2013 ... hôtes de Dacryodes edulis alors que les autres ligneux cultivés étaient parasités par Phragmanthera capitata. Le taux moyen de ...... Mistletoes as parasites host specificity and speciation. Trends. Ecology and Evolution 13: 101-105. Peakall R, 1994. Interactions between orchids and ants. In: Orchid ...

  2. Aqueous misdirection following pars plana vitrectomy and silicone oil injection.

    Science.gov (United States)

    Ghoraba, Hammouda H; Ghali, Ali Ahmed; Mansour, Hosam Othman

    2015-01-01

    To report a retrospective series of seven phakic eyes of seven patients suffering from a malignant glaucoma-like syndrome following pars plana vitrectomy and silicone oil (SO) injection. Seven eyes with retinal detachment treated with pars plana vitrectomy with or without scleral buckling with SO tamponade. This was followed by cataract extraction to manage the elevated intraocular pressure (IOP). This was a retrospective review of seven cases that received pars plana vitrectomy and SO with or without scleral buckling for different causes of retinal detachment (three were rhegmatogenous and four were tractional). After a period ranging from 1 week to 1 month, they presented with malignant glaucoma-like manifestations; high IOP, shallow axial anterior chamber, and remarkable decrease of visual acuity. Atropine eye drops and anti-glaucoma medical treatment (topical and systemic) had been tried but failed to improve the condition. Dramatic decrease of IOP and deepening of the axial anterior chamber was observed in all cases in the first postoperative day after phacoemulsification and posterior chamber foldable intraocular lens implantation with posterior capsulotomy. Aqueous misdirection syndrome may be observed following pars plana vitrectomy and SO tamponade. This must be differentiated from other causes of post vitrectomy glaucoma. Cataract extraction with posterior capsulotomy controls the condition.

  3. BOREAS RSS-10 TOMS Circumpolar One-Degree PAR Images

    Science.gov (United States)

    Dye, Dennis G.; Holben, Brent; Nickeson, Jaime (Editor); Hall, Forrest G. (Editor); Smith, David E. (Technical Monitor)

    2000-01-01

    The Boreal Ecosystem-Atmosphere Study (BOREAS) Remote Sensing Science (RSS)-10 team investigated the magnitude of daily, seasonal, and yearly variations of Photosynthetically Active Radiation (PAR) from ground and satellite observations. This data set contains satellite estimates of surface-incident PAR (400-700 nm, MJ/sq m) at one-degree spatial resolution. The spatial coverage is circumpolar from latitudes of 41 to 66 degrees north. The temporal coverage is from May through September for years 1979 through 1989. Eleven-year statistics are also provided: (1) mean, (2) standard deviation, and (3) coefficient of variation for 1979-89. The PAR estimates were derived from the global gridded ultraviolet reflectivity data product (average of 360, 380 nm) from the Nimbus-7 Total Ozone Mapping Spectrometer (TOMS). Image mask data are provided for identifying the boreal forest zone, and ocean/land and snow/ice-covered areas. The data are available as binary image format data files. The PAR data are available from the Earth Observing System Data and Information System (EOSDIS) Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC). The data files are available on a CD-ROM (see document number 20010000884).

  4. Den eneste ene - hvordan etniske minoritetsunge i Danmark danner par

    DEFF Research Database (Denmark)

    Singla, Rashmi

    Rapporten handler om etniske unges måde at danne par på. Rapporten er baseret på en større empirisk undersøgelse gennemført af forfatteren i 2003 og er desuden inspireret af forfatterens egen ph.d.-afhandling: "Ungdom, etnicitet og psychosocial intervention" (2000, Københavns Universitet...

  5. Ankaferd Blood Stopper induces apoptosis and regulates PAR1 and ...

    African Journals Online (AJOL)

    Mine Mumcuoglu

    2014-12-16

    Dec 16, 2014 ... Expression analyses of PAR1, EPCR and p21 upon ABS treatment were performed by quantitative real time PCR. Annexin V ... 2014 Production and hosting by Elsevier B.V. on behalf of Ain Shams University. 1. Introduction. Ankaferd Blood ..... effects of Ankaferd as an alternative medicine. Altern Med Rev.

  6. Priapisme induit par la chlorpromazine: A propos de deux cas

    Directory of Open Access Journals (Sweden)

    I. Marrag

    2016-06-01

    Le priapisme veineux est une urgence urologique. Il constitue un des effets secondaires des neuroleptiques parmi les quels la chlorpromazine. Cet effet iatrogène, qui est rare mais grave, doit être connu par les cliniciens afin d’être mieux prévenu pour éviter les séquelles érectiles.

  7. Evaluation De L'activite Antifongique Par Bioautographie De ...

    African Journals Online (AJOL)

    Evaluation De L'activite Antifongique Par Bioautographie De Quelques Plantes Medicinales De Côte D'ivoire Contre Deux Formes Speciales De Fusarium Oxysporum. ... Fusariosis are among the most devastating diseases in crops, especially in tomato, cotton and banana. This study was carried out in order to identify ...

  8. PH motifs in PAR1&2 endow breast cancer growth.

    Science.gov (United States)

    Kancharla, A; Maoz, M; Jaber, M; Agranovich, D; Peretz, T; Grisaru-Granovsky, S; Uziely, B; Bar-Shavit, R

    2015-11-24

    Although emerging roles of protease-activated receptor1&2 (PAR1&2) in cancer are recognized, their underlying signalling events are poorly understood. Here we show signal-binding motifs in PAR1&2 that are critical for breast cancer growth. This occurs via the association of the pleckstrin homology (PH) domain with Akt/PKB as a key signalling event of PARs. Other PH-domain signal-proteins such as Etk/Bmx and Vav3 also associate with PAR1 and PAR2 through their PH domains. PAR1 and PAR2 bind with priority to Etk/Bmx. A point mutation in PAR2, H349A, but not in R352A, abrogates PH-protein association and is sufficient to markedly reduce PAR2-instigated breast tumour growth in vivo and placental extravillous trophoblast (EVT) invasion in vitro. Similarly, the PAR1 mutant hPar1-7A, which is unable to bind the PH domain, reduces mammary tumours and EVT invasion, endowing these motifs with physiological significance and underscoring the importance of these previously unknown PAR1 and PAR2 PH-domain-binding motifs in both pathological and physiological invasion processes.

  9. La Féminité Vue Par Simone De Beauvoir : Une Lecture Du ...

    African Journals Online (AJOL)

    Simone de Beauvoir est une féministe et philosophe française dont la théorie féministe et idéologie philosophique ont beaucoup marqué le 20e siècle. Elle fait à travers son oeuvre théorique, Le deuxième sexe publiée en 1949, une dénonciation très rigoureuse de l'infériorisation et de l'assujettissement de l'être féminin (la ...

  10. Los yunteros de Extremadura (Marqués de Villa-Alcázar, 1936: un eslabón recuperado del documental cinematográfico de la Segunda República

    Directory of Open Access Journals (Sweden)

    Poyato Sánchez, Pedro

    2014-09-01

    Full Text Available The recent recovery of Los yunteros de Extremadura (The Plowmen of Extremadura, a documentary produced by the Instituto de Reforma Agraria in 1936, is a step forward in the reconstruction of the filmography of the Second Spanish Republic, a crucial period in the history of the propaganda documentary. Requested by the Republican Propaganda Agency in Paris, Los yunteros de Extremadura was nevertheless especially critical of the Republican government. This study analyzes this question as well as the iconography of the film and its interesting dialogue with Buñuel’s Las Hurdes/ Land Without Bread (1933 / 1936.La reciente recuperación de Los yunteros de Extremadura, documental producido por el Instituto de Reforma Agraria en 1936, ha supuesto un paso más en la reconstrucción de la filmografía de la Segunda República, periodo crucial en la historia del documental del propaganda. Requerida por la Delegación de Propaganda en París, Los yunteros de Extremadura es, sin embargo, una película especialmente crítica con la República. El presente trabajo se ocupa de esta cuestión como también de la iconografía del filme y del interesante diálogo que este mantiene con Las Hurdes/ Tierra sin pan, de Luis Buñuel (1933/1936.

  11. Comparison of Extrapolation and Interpolation Methods for Estimating Daily Photosynthetically Active Radiation (PAR)

    NARCIS (Netherlands)

    Wu, G.; Skidmore, A.K.; Leeuw, de J.; Liu, X.; Prins, H.H.T.

    2010-01-01

    Measurements of photosynthetically active radiation (PAR), which are indispensable for simulating plant growth and productivity, are generally very scarce. This study aimed to compare two extrapolation and one interpolation methods for estimating daily PAR reaching the earth surface within the

  12. Alpine grassland fPAR change over the Northern Tibetan Plateau from 2002 to 2011

    National Research Council Canada - National Science Library

    CHEN Bao-Xiong ZHANG Xian-Zhou SUN Yu-Fang WANG Jing-Sheng HE Yong-Tao

    2017-01-01

    ...) and traditional in situ fPAR observation for measuring the in situ point fPAR of very short alpine grass vegetation were compared, and the Moderate Resolution Imaging Spectroradiometer (MODIS...

  13. Contamination des eaux de puits traditionnels par les nitrates sur le ...

    African Journals Online (AJOL)

    SARAH

    30 juin 2014 ... Objectif : La contamination des eaux souterraines par les nitrates ... Methodology and Results: the origin of nitrate water from traditional wells of Lobo's watershed was ..... avec celles faites par Hudak (1999) au Texas et Liu et.

  14. Apoplejía pituitaria con parálisis del III par craneal: Reporte de caso

    Directory of Open Access Journals (Sweden)

    Miguel Pinto Valdivia

    2011-10-01

    Full Text Available Se describe el caso de un varón de 65 años de edad, sin antecedentes patológicos de importancia, que acudió a emergencia del Hospital Nacional Cayetano Heredia por presentar cefalea intensa y ptosis palpebral izquierda. El examen físico mostró parálisis aislada del III par craneal izquierdo. Los análisis de laboratorio mostraron hiponatremia e hipopituitarismo y la resonancia magnética nuclear un adenoma pituitario con áreas de hemorragia e invasión de los senos cavernosos. El tratamiento incluyó glucocorticoides y descompresión quirúrgica transesfenoidal. La anatomía patológica confirmó el diagnóstico de infarto hemorrágico de un adenoma pituitario. El paciente fue dado de alta con terapia sustitutiva de levotiroxina y prednisona. La ptosis palpebral izquierda se recuperó en forma parcial. La apoplejía pituitaria es un síndrome clínico producido por un proceso expansivo dentro de la silla turca, secundario a hemorragia o infarto de un adenoma pituitario, que se caracteriza por cefalea, déficit visual, oftalmoplejía y alteración del nivel de conciencia. Este proceso expansivo puede comprimir los pares craneales en los senos cavernosos, produciendo diferentes grados de parálisis de los músculos oculomotores. La parálisis aislada del III par craneal es rara.(Rev Med Hered 2011;22:186-189.

  15. ParCAT: A Parallel Climate Analysis Toolkit

    Science.gov (United States)

    Haugen, B.; Smith, B.; Steed, C.; Ricciuto, D. M.; Thornton, P. E.; Shipman, G.

    2012-12-01

    Climate science has employed increasingly complex models and simulations to analyze the past and predict the future of our climate. The size and dimensionality of climate simulation data has been growing with the complexity of the models. This growth in data is creating a widening gap between the data being produced and the tools necessary to analyze large, high dimensional data sets. With single run data sets increasing into 10's, 100's and even 1000's of gigabytes, parallel computing tools are becoming a necessity in order to analyze and compare climate simulation data. The Parallel Climate Analysis Toolkit (ParCAT) provides basic tools that efficiently use parallel computing techniques to narrow the gap between data set size and analysis tools. ParCAT was created as a collaborative effort between climate scientists and computer scientists in order to provide efficient parallel implementations of the computing tools that are of use to climate scientists. Some of the basic functionalities included in the toolkit are the ability to compute spatio-temporal means and variances, differences between two runs and histograms of the values in a data set. ParCAT is designed to facilitate the "heavy lifting" that is required for large, multidimensional data sets. The toolkit does not focus on performing the final visualizations and presentation of results but rather, reducing large data sets to smaller, more manageable summaries. The output from ParCAT is provided in commonly used file formats (NetCDF, CSV, ASCII) to allow for simple integration with other tools. The toolkit is currently implemented as a command line utility, but will likely also provide a C library for developers interested in tighter software integration. Elements of the toolkit are already being incorporated into projects such as UV-CDAT and CMDX. There is also an effort underway to implement portions of the CCSM Land Model Diagnostics package using ParCAT in conjunction with Python and gnuplot. Par

  16. Attic cholesteatoma with tiny retraction of pars flaccida.

    Science.gov (United States)

    Lee, Jun Ho; Hong, Seok Min; Kim, Chang Woo; Park, Yeo Hoon; Baek, So-Hye

    2015-04-01

    This clinical study was performed to analyze the characteristics of attic cholesteatoma occurring behind a tiny retraction of the pars flaccida, which was classified as Tos type I or II and had an intact pars tensa of the tympanic membrane. The clinical records of patients who underwent tympanomastoidectomy for attic cholesteatoma at a tertiary care referral center (Kangdong Sacred Heart Hospital of Seoul, Korea) between March 2004 and December 2012 were retrospectively reviewed. Eleven patients (five men and six women) who underwent tympanomastoidectomy between March 2004 and December 2012 for attic cholesteatoma occurring behind a tiny attic retraction were included. The mean age of patients was 41.1 years (range 20-58 years) and the mean duration of follow-up was 29.5 months (range 13-52 months). Every patient had a unilateral cholesteatoma, and the opposite side was normal except in one patient. Hearing loss was the most common symptom, followed by earfullness and otalgia. Five patients had type I attic retraction, and six patients had type II attic retraction. No patient had definite scutum erosion. Interestingly, during regular postoperative checkup, one patient was found incidentally for the opposite ear. Six patients had a cholesteatoma sac that was separated from the pars flaccida, whereas in five patients it was in contact with the pars flaccida but was easily separated. Six patients had a limited cholesteatoma within the epitympanum, and five patients had extension beyond the epitympanum. The average air-bone gap was 24.3±10.1dB before the operation and 14.2±6.6dB after the operation. Every patient had an intact tympanic membrane without retraction pocket postoperatively. There was no recurrence of cholesteatoma during follow-up. The rate of attic cholesteatomas occurring behind a tiny retraction of the pars flaccida was 7.7% (11 of 142 patients with attic cholesteatoma). Attic retractions must be followed closely using endoscopy, microscopy, and

  17. The Urokinase Receptor (uPAR) Facilitates Clearance of Borrelia burgdorferi

    NARCIS (Netherlands)

    Hovius, Joppe W. R.; Bijlsma, Maarten F.; van der Windt, Gerritje J. W.; Wiersinga, W. Joost; Boukens, Bastiaan J. D.; Coumou, Jeroen; Oei, Anneke; de Beer, Regina; de Vos, Alex F.; van 't Veer, Cornelis; van Dam, Alje P.; Wang, Penghua; Fikrig, Erol; Levi, Marcel M.; Roelofs, Joris J. T. H.; van der Poll, Tom

    2009-01-01

    The causative agent of Lyme borreliosis, the spirochete Borrelia burgdorferi, has been shown to induce expression of the urokinase receptor (uPAR); however, the role of uPAR in the immune response against Borrelia has never been investigated. uPAR not only acts as a proteinase receptor, but can

  18. 78 FR 42584 - Additional Designation of Aluminat, Pars Amayesh Sanaat Kish, Parviz Khaki, Pishro Systems...

    Science.gov (United States)

    2013-07-16

    ... Additional Designation of Aluminat, Pars Amayesh Sanaat Kish, Parviz Khaki, Pishro Systems Research Company... the Treasury and the Attorney General, has determined that Aluminat, Pars Amayesh Sanaat Kish, Parviz..., Parcham Street, Arak, Iran PARS Amayesh Sanaat Kish AKA: PASK AKA: Vacuumkaran AKA: Vacuum Karan AKA...

  19. Five-Port Combined Limbal and Pars Plana Vitrectomy for Infectious Endophthalmitis.

    Science.gov (United States)

    Xu, Kunyong; Chin, Eric K; Almeida, David R P

    2016-01-01

    Pars plana vitrectomy for acute infectious endophthalmitis can be challenging due to severe inflammation in the anterior chamber creating significant media opacity. We describe a surgical technique combining limbal based vitrectomy and pars plana vitrectomy to manage acute infectious endophthalmitis. Limbal based vitrectomy facilitates removal of anterior chamber fibrin and inflammatory membranes for safe and optimal posterior pars plana vitrectomy.

  20. 12 CFR 925.19 - Par value and price of stock.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Par value and price of stock. 925.19 Section... ASSOCIATES MEMBERS OF THE BANKS Stock Requirements § 925.19 Par value and price of stock. The capital stock of each Bank shall be sold at par, unless the Board has fixed a higher price. ...

  1. Neo-Positivist Intrusions, Post-Qualitative Challenges, and PAR's Generative Indeterminacies

    Science.gov (United States)

    Miller, Janet L.

    2017-01-01

    Although committed to PAR's overarching aspirations, many advocates also have noted myriad complexities of engaging in PAR, where ambiguities and disarrays--all kinds of inconclusive evidence--can proliferate. Uncertainties especially can erupt if PAR education-focused projects are positioned, oxymoronically, as expected to produce "high…

  2. Is There an "F" in Your PAR? Understanding, Teaching and Doing Action Research

    Science.gov (United States)

    Lorenzetti, Liza; Walsh, Christine Ann

    2014-01-01

    Participatory Action Research (PAR) is increasingly recognized within academic research and pedagogy. What are the benefits of including feminism within participatory action research and teaching? In responding to this question, we discuss the similarities and salient differences between PAR and feminist informed PAR (FPAR). There are eight themes…

  3. A network of cis and trans interactions is required for ParB spreading

    Science.gov (United States)

    Song, Dan; Rodrigues, Kristen; Graham, Thomas G.W.

    2017-01-01

    Abstract Most bacteria utilize the highly conserved parABS partitioning system in plasmid and chromosome segregation. This system depends on a DNA-binding protein ParB, which binds specifically to the centromere DNA sequence parS and to adjacent non-specific DNA over multiple kilobases in a phenomenon called spreading. Previous single-molecule experiments in combination with genetic, biochemical and computational studies have argued that ParB spreading requires cooperative interactions between ParB dimers including DNA bridging and possible nearest-neighbor interactions. A recent structure of a ParB homolog co-crystallized with parS revealed that ParB dimers tetramerize to form a higher order nucleoprotein complex. Using this structure as a guide, we systematically ablated a series of proposed intermolecular interactions in the Bacillus subtilis ParB (BsSpo0J) and characterized their effect on spreading using both in vivo and in vitro assays. In particular, we measured DNA compaction mediated by BsSpo0J using a recently developed single-molecule method to simultaneously visualize protein binding on single DNA molecules and changes in DNA conformation without protein labeling. Our results indicate that residues acting as hubs for multiple interactions frequently led to the most severe spreading defects when mutated, and that a network of both cis and trans interactions between ParB dimers is necessary for spreading. PMID:28407103

  4. Avis 6 sur la formation à et par la recherche

    OpenAIRE

    Centre de Coopération Internationale en Recherche Agronomique pour le Développement

    2015-01-01

    La formation à et par la recherche s’appuie sur la démarche scientifique, les savoirs, les connaissances à transmettre, tout autant que l’acquisition de compétences en gestion de projet, de capacités au travail en équipe, d’une ouverture d’esprit qui favorisera la créativité. Elle doit permettre de prendre conscience de la responsabilité endossée par la communauté scientifique vis-à-vis de la société et de l’environnement, mais aussi préparer l’avenir professionnel de celle ou de celui qu...

  5. La syphilis congenitale revelee par une fracture spontanee

    Directory of Open Access Journals (Sweden)

    Mounia Lakhdar Idrissi

    2011-11-01

    Full Text Available Alors qu�elle est actuellement oubliee dans les pays developpes, la syphilis congenitale se voit encore chez nous faute du depistage antenatal. Ses formes cliniques sont polymorphes et orientent a tord vers d�autres pathologies surtout en periode neonatale. Le diagnostic n�est donc pas toujours facile. La revelation d�une syphilis congenitale par une fracture spontanee est exceptionnellement decrite. Nous rapportons dans ce travail le cas d�un nourrisson de 2 mois ramene en consultation pour limitation douloureuse des mouvements du bras droit. Le diagnostic est evoque sur les donnees radiologiques et confirme par la serologie syphilitique. Le traitement a repose essentiellement sur l�administration de la penicilline G avec une bonne evolution clinique.

  6. Chaos quantique avec des atomes refroidis par laser

    OpenAIRE

    Lignier, Hans; Thommen, Quentin; Chabé, Julien; Delande, Dominique; Garreau, Jean Claude; Szriftgiser, Pascal; Zehnlé, Véronique

    2005-01-01

    Conférence présentée au 5e Colloque "Chaos Temporel et Chaos Spatio-Temporel", Le Havre, décembre 2005; La dynamique des systèmes chaotiques est le sujet de nombreuses études en physique. Après les percées accomplies par Poincaré au début du XXe siècle, les idées se sont fixées avec la découverte par Lorenz dans les années 60 de la ``sensibilité aux conditions initiales'', qui découle de la non linéarité des équations du mouvement. Plusieurs difficultés se présentent cependant quand l'on tent...

  7. Adoption d'innovations agricoles par le truchement de services ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    L'agriculture est le fer de lance de nombreuses économies en développement, mais la capacité des petits agriculteurs privés de ressources à adopter des innovations agricoles est freinée par le manque d'accès à des services financiers adéquats. Des experts internationaux estiment qu'il existe un manque à gagner ...

  8. Notre experience de la myelopathie par arthrose cervicale a Dakar ...

    African Journals Online (AJOL)

    ... (p=0,48 > 0,05) ne semblent pas influencer la récupération fonctionnelle post opératoire. Conclusion L'incidence de myélopathie par arthrose cervicale apparaît au Sénégal comme une pathologie sousestimée. La laminectomie décompressive sans instrumentation postérieure constitue notre choix thérapeutique majeur.

  9. Démarginalisation des travailleurs pauvres par le droit | CRDI ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Démarginalisation des travailleurs pauvres par le droit. Cette subvention appuiera une étude approfondie de la situation, des revendications et des combats juridiques des travailleurs pauvres (plus particulièrement des femmes) membres de l'économie non structurée au Ghana, au Pérou et en Thaïlande. On aura recours à ...

  10. Inondations dans la banlieue de Dakar : vers une adaptation par ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Inondations dans la banlieue de Dakar : vers une adaptation par des améliorations du bâti, des infrastructures et de la gouvernance locale. La gestion des inondations représente un des défis majeurs du continent africain en regard des changements climatiques. Une urbanisation effrénée, un étalement urbain hors de ...

  11. Anesthesie topique par oxybuprocaine dans la chirurgie de cataracte

    African Journals Online (AJOL)

    Étude prospective portant sur 100 patients opérés pour cataracte simple stade I, II et III sous anesthésie topique par instillation de quatre gouttes d'Oxybuprocaïne 0.4% collyre, nous avons analysé les complications rencontrées et le confort des patients à l'aide de l'échelle visuelle analogique. La tolérance du patient durant ...

  12. La parálisis cerebral en el contexto escolar /

    OpenAIRE

    Rodríguez Gimeno, Ignacio

    2012-01-01

    Se analiza el concepto de parálisis cerebral, los diferentes tipos y sus características. También las adaptaciones que se deben realizar a nivel de centro, de aula, y a nivel individual para estos alumnos, conocer los diferentes sistemas alternativos y aumentativos de comunicación y aplicar las ayudas técnicas que precisa este alumnado

  13. Irrigation endoscopic assisted percutaneous pars repair: technical note.

    Science.gov (United States)

    Soliman, Hesham Magdi

    2016-10-01

    Spondylolysis is a common source of low back pain in children and adolescents. Despite the benign natural history of the pathology, however, it should be noted that as part of conservative management many young individuals are forced to discontinue their routine sports activities. In cases resistant to conservative management, direct repair of a pars defect is considered a safe and effective option in young adults with preserved discs. Recently, some reports of minimally invasive pars repair have been described, aiming at preservation of the paraspinal muscles and improvement of the postoperative course. The study aimed to present a new minimally invasive endoscopic technique for pars repair. This is a technical note. Seven consecutive patients complaining of back pain due to spondylolysis and resistant to conservative management were included. Outcome was assessed using the modified Macnab criteria, and bone union was assessed on postoperative radiographs. Two portals of 0.5 cm were used on each side, 1 cm lateral to the midline. One portal is used for the endoscope and the second for the surgical instruments. Following endoscopic debridement of the defect, the inferior portal was used for percutaneous placement of the Buck screw. There was no source for external funding for this study and no potential conflict of interest to disclose. Outcome was excellent in six cases and good in one case. All patients returned back to their normal level of activities. Complete radiographic union was seen in all patients. The mean period of postoperative hospital stay and follow-up was 8 hours and 21 months, respectively. The irrigation endoscopic technique has previously shown promising results in lumbar discectomy and laminectomy. Similar results were observed in the current study on the ability to achieve minimally invasive pars defect debridement and a smooth postoperative course. This is a pilot study and larger patient series and different surgeons' experience are

  14. Excision Les jeunes changent l'Afrique par les TIC

    International Development Research Centre (IDRC) Digital Library (Canada)

    Encadré 8 - Recherche sur les MGF : des concepts et méthodes à actualiser. 58. Encadré 9 - Faire comme les grands, quand on est jeune. 69. Encadré 10 - Etre un aîné, le rêve. 71. Encadré 11 - Le paradigme transdisciplinaire vu par les jeunes. 78. Encadré 12 - Citoyenneté au féminin/masculin. 83. Encadré 13 - Le projet, ...

  15. Neural crest specification by noncanonical Wnt signaling and PAR-1

    Science.gov (United States)

    Ossipova, Olga; Sokol, Sergei Y.

    2011-01-01

    Neural crest (NC) cells are multipotent progenitors that form at the neural plate border, undergo epithelial-mesenchymal transition and migrate to diverse locations in vertebrate embryos to give rise to many cell types. Multiple signaling factors, including Wnt proteins, operate during early embryonic development to induce the NC cell fate. Whereas the requirement for the Wnt/β-catenin pathway in NC specification has been well established, a similar role for Wnt proteins that do not stabilize β-catenin has remained unclear. Our gain- and loss-of-function experiments implicate Wnt11-like proteins in NC specification in Xenopus embryos. In support of this conclusion, modulation of β-catenin-independent signaling through Dishevelled and Ror2 causes predictable changes in premigratory NC. Morpholino-mediated depletion experiments suggest that Wnt11R, a Wnt protein that is expressed in neuroectoderm adjacent to the NC territory, is required for NC formation. Wnt11-like signals might specify NC by altering the localization and activity of the serine/threonine polarity kinase PAR-1 (also known as microtubule-associated regulatory kinase or MARK), which itself plays an essential role in NC formation. Consistent with this model, PAR-1 RNA rescues NC markers in embryos in which noncanonical Wnt signaling has been blocked. These experiments identify novel roles for Wnt11R and PAR-1 in NC specification and reveal an unexpected connection between morphogenesis and cell fate. PMID:22110058

  16. First (18)F-labeled ligand for PET imaging of uPAR

    DEFF Research Database (Denmark)

    Persson, Morten; Liu, Hongguang; Madsen, Jacob

    2013-01-01

    Urokinase-type plasminogen activator receptor (uPAR) is overexpressed in human prostate cancer and uPAR has been found to be associated with metastatic disease and poor prognosis. AE105 is a small linear peptide with high binding affinity to uPAR. We synthesized an N-terminal NOTA-conjugated vers......-conjugated version (NOTA-AE105) for development of the first (18)F-labeled uPAR positron-emission-tomography PET ligand using the Al(18)F radiolabeling method. In this study, the potential of (18)F-AlF-NOTA-AE105 to specifically target uPAR-positive prostate tumors was investigated....

  17. The Role of Neurosecretory Neurons in the Pars Intercerebralis and Pars Lateralis in Reproductive Diapause of the Blowfly, Protophormia terraenovae

    Science.gov (United States)

    Shiga, S.; Numata, H.

    Microlesions of the brain were made to examine the role of neurosecretory neurons in the pars intercerebralis (PI) and pars lateralis (PL) in the induction of reproductive diapause of the female blowfly Protophormia terraenovae. Under both diapause-inducing (LD 12 : 12, 20° C) and diapause-averting conditions (LD 18 : 6, 25° C), the ovaries invariably failed to develop when the PI was removed. When the PL was removed bilaterally, the ovaries developed in most of the females, irrespective of the rearing conditions. Removal of the PL prevented females from entering reproductive diapause. These results show that certain neurosecretory neurons in the PI are necessary for vitellogenesis, and that the PL contains inhibitory neurons which suppress vitellogenesis during reproductive diapause.

  18. Characterization of a Par j 1/Par j 2 mutant hybrid with reduced allergenicity for immunotherapy of Parietaria allergy.

    Science.gov (United States)

    Bonura, A; Passantino, R; Costa, M A; Montana, G; Melis, M; Bondì, M Luisa; Butteroni, C; Barletta, B; Corinti, S; Di Felice, G; Colombo, P

    2012-03-01

    Parietaria pollen is one of the major cause of pollinosis in the southern Europe. Specific immunotherapy is the only treatment able to modify the natural outcome of the disease restoring a normal immunity against allergens. We designed a recombinant molecule (PjEDloop1) comprised of genetic-engineered variants of the major allergens of the Parietaria pollen (Par j 2/Par j 1). Purity and chemical-physical properties of the derivative were analysed by RP-HPLC chromatography and Photon Correlation Spectroscopy. Immunological activity was evaluated by means of Western blotting, ELISA inhibition and PBMC proliferation assay in 10 Parietaria allergic patients. Basophil activation was studied in six subjects. The immunogenicity of the hybrid was studied looking at the immune responses induced in a mouse model of sensitization. The PjEDloop1 hybrid was produced as a purified recombinant protein with high stability in solution. Western blot, ELISA inhibition and basophil activation test showed that the PjEDloop1 displays a remarkable reduced IgE binding and anaphylactic activity. CD3 reactivity was conserved in all patients. Mice immunization with the rPjEDloop1 induced antibodies and T cell responses comparable to that obtained by the wild type allergens. Such antibodies shared the specificities to rPar j 1 and rPar j 2 with human IgE antibodies. Our results demonstrated that a mutant hybrid expressing genetically engineered forms of the major P. judaica allergens displayed reduced allergenicity and retained T cell reactivity for the induction of protective antibodies in vaccination approaches for the treatment of Parietaria pollinosis. © 2011 Blackwell Publishing Ltd.

  19. PAR Genes: Molecular Probes to Pathological Assessment in Breast Cancer Progression

    Directory of Open Access Journals (Sweden)

    Beatrice Uziely

    2011-01-01

    Full Text Available Taking the issue of tumor categorization a step forward and establish molecular imprints to accompany histopathological assessment is a challenging task. This is important since often patients with similar clinical and pathological tumors may respond differently to a given treatment. Protease-activated receptor-1 (PAR1, a G protein-coupled receptor (GPCR, is the first member of the mammalian PAR family consisting of four genes. PAR1 and PAR2 play a central role in breast cancer. The release of N-terminal peptides during activation and the exposure of a cryptic internal ligand in PARs, endow these receptors with the opportunity to serve as a “mirror-image” index reflecting the level of cell surface PAR1&2-in body fluids. It is possible to use the levels of PAR-released peptide in patients and accordingly determine the choice of treatment. We have both identified PAR1 C-tail as a scaffold site for the immobilization of signaling partners, and the critical minimal binding site. This binding region may be used for future therapeutic modalities in breast cancer, since abrogation of the binding inhibits PAR1 induced breast cancer. Altogether, both PAR1 and PAR2 may serve as molecular probes for breast cancer diagnosis and valuable targets for therapy.

  20. Expression of Partitioning Defective 3 (Par-3 for Predicting Extrahepatic Metastasis and Survival with Hepatocellular Carcinoma

    Directory of Open Access Journals (Sweden)

    Jun-Yang Liou

    2013-01-01

    Full Text Available Partitioning defective 3 (Par-3, a crucial component of partitioning-defective complex proteins, controls cell polarity and contributes to cell migration and cancer cell epithelial-to-mesenchymal transition. However, the clinical relevance of Par-3 in tumor progression and metastasis has not been well elucidated. In this study, we investigated the impact and association of Par-3 expression and clinical outcomes with hepatocellular carcinoma (HCC. We first confirmed that Par-3 was abundantly expressed in HCC cell lines by Western blot analysis. We used immunohistochemistry to analyze the association of Par-3 expression and clinicopathological characteristics in primary and subsequent metastatic tumors of patients with HCC. Par-3 was overexpressed in 47 of 111 (42.3% primary tumors. Increased expression of Par-3 in primary tumors predicted an increased five-year cumulative incidence of extrahepatic metastasis. In addition, multivariate analysis revealed that Par-3 overexpression was an independent risk factor of extrahepatic metastasis. Increased Par-3 expression in primary tumors was associated with poor five-year overall survival rates and was an independent prognostic factor on Cox regression analysis. In conclusion, we show for the first time that increased Par-3 expression is associated with distant metastasis and poor survival rates in patients with HCC. Par-3 may be a novel prognostic biomarker and therapeutic target for HCC.

  1. Effect of PAR-2 Deficiency in Mice on KC Expression after Intratracheal LPS Administration

    Directory of Open Access Journals (Sweden)

    Julie C. Williams

    2011-01-01

    Full Text Available Protease activated receptors (PAR have been shown to play a role in inflammation. PAR-2 is expressed by numerous cells in the lung and has either proinflammatory, anti-inflammatory, or no effect depending on the model. Here, we examined the role of PAR-2 in a model of LPS-induced lung inflammation. We found that PAR-2-deficient mice had significantly less KC expression in bronchial lavage fluid compared with wild-type mice but there was no difference in MIP-2 or TNF-α expression. We also found that isolated alveolar and resident peritoneal macrophages lacking PAR-2 showed a similar deficit in KC after LPS stimulation without differences in MIP-2 or TNF-α. Infiltration of neutrophils and macrophages into the lung following LPS administration was not affected by an absence of PAR-2. Our results support the notion that PAR-2 plays a role in LPS activation of TLR4 signaling in macrophages.

  2. Caracterisation et transformation par hydroviscoreduction du brut lourd de Doba/Tchad: Strategie de pompage par pipeline

    Science.gov (United States)

    Dehkissia, Soumaine

    Le projet de la these est defini par rapport a la recente exploitation commerciale du brut lourd de Doba, une region du Tchad, pays enclave d'ou le brut ainsi produit doit etre achemine par pipeline. Le but dans ce travail est de caracteriser ce brut et de determiner une strategie thermique de transformation en vue de reduire de la facon la plus economique, la viscosite du brut pour permettre son transport par pipeline. Concernant la partie caracterisation, nous avons utilise entre autres, un rheometre rotatif et des analyseurs (LECO CHN-2000 & LECO S-144DR Elemental Analyzers) pour evaluer respectivement la viscosite et les elements tels que C, H, N, O, S. Sur la base des methodes de transformation disponibles, nous avons determine une strategie simple de transformation thermique par hydroviscoreduction , strategie dans laquelle, la fraction legere du brut comme le naphta (80--180°C), pourrait constituer la source d'hydrogene a utiliser. Le brut de Doba, a une faible teneur en soufre (0.14%) et sa densite specifique de 0.940 a 15.6°C, soit 18.8° API, le classe parmi les bruts lourds. Outre le point initial se situant a 85°C, la distillation du brut a revele que la fraction distillant avant 250°C ne represente que 10% (v/v) et que le craquage thermique du substrat debute a 300°C. Par ailleurs, outre son caractere Newtonien, les densites specifiques de la fraction lourde de l'essence (100--200°C) et de la fraction distillant au-dessus 350°C, etant respectivement de 0.813 (0.813 > 0.800) et de 0.951 (0.930 vieillissement meme apres deux mois, ce qui correspond au temps de sejour du brut dans le pipeline. L'etude montre aussi que les asphaltenes du brut traite en absence d'un catalyseur sont moins aromatiques que ceux du brut non-traite.

  3. The double par locus of virulence factor pB171: DNA segregation is correlated with oscillation of ParA

    DEFF Research Database (Denmark)

    Ebersbach, G; Gerdes, K; Charbon, Gitte Ebersbach

    2001-01-01

    Prokaryotic plasmids and chromosomes encode partitioning (par) loci that segregate DNA to daughter cells before cell division. Recent database analyses showed that almost all known par loci encode an ATPase and a DNA-binding protein, and one or more cis-acting regions where the proteins act. All...

  4. Transcriptional profiling of ParA and ParB mutants in actively dividing cells of an opportunistic human pathogen Pseudomonas aeruginosa.

    Directory of Open Access Journals (Sweden)

    Aneta A Bartosik

    Full Text Available Accurate chromosome segregation to progeny cells is a fundamental process ensuring proper inheritance of genetic material. In bacteria with simple cell cycle, chromosome segregation follows replication initiation since duplicated oriC domains start segregating to opposite halves of the cell soon after they are made. ParA and ParB proteins together with specific DNA sequences are parts of the segregation machinery. ParA and ParB proteins in Pseudomonas aeruginosa are important for optimal growth, nucleoid segregation, cell division and motility. Comparative transcriptome analysis of parA null and parB null mutants versus parental P. aeruginosa PAO1161 strain demonstrated global changes in gene expression pattern in logarithmically growing planktonic cultures. The set of genes similarly affected in both mutant strains is designated Par regulon and comprises 536 genes. The Par regulon includes genes controlled by two sigma factors (RpoN and PvdS as well as known and putative transcriptional regulators. In the absence of Par proteins, a large number of genes from RpoS regulon is induced, reflecting the need for slowing down the cell growth rate and decelerating the metabolic processes. Changes in the expression profiles of genes involved in c-di-GMP turnover point out the role of this effector in such signal transmission. Microarray data for chosen genes were confirmed by RT-qPCR analysis. The promoter regions of selected genes were cloned upstream of the promoter-less lacZ gene and analyzed in the heterologous host E. coliΔlac. Regulation by ParA and ParB of P. aeruginosa was confirmed for some of the tested promoters. Our data demonstrate that ParA and ParB besides their role in accurate chromosome segregation may act as modulators of genes expression. Directly or indirectly, Par proteins are part of the wider regulatory network in P. aeruginosa linking the process of chromosome segregation with the cell growth, division and motility.

  5. Validation of MODIS and GEOV1 fPAR Products in a Boreal Forest Site in Finland

    Directory of Open Access Journals (Sweden)

    Titta Majasalmi

    2015-01-01

    Full Text Available Remote sensing of the fraction of absorbed Photosynthetically Active Radiation (fPAR has become a timely option to monitor forest productivity. However, only a few studies have had ground reference fPAR datasets containing both forest canopy and understory fPAR from boreal forests for the validation of satellite products. The aim of this paper was to assess the performance of two currently available satellite-based fPAR products: MODIS fPAR (MOD15A2, C5 and GEOV1 fPAR (g2_BIOPAR_FAPAR, as well as an NDVI-fPAR relationship applied to the MODIS surface reflectance product and a Landsat 8 image, in a boreal forest site in Finland. Our study area covered 16 km2 and field data were collected from 307 forest plots. For all plots, we obtained both forest canopy fPAR and understory fPAR. The ground reference total fPAR agreed better with GEOV1 fPAR than with MODIS fPAR, which showed much more temporal variation during the peak-season than GEOV1 fPAR. At the chosen intercomparison date in peak growing season, MODIS NDVI based fPAR estimates were similar to GEOV1 fPAR, and produced on average 0.01 fPAR units smaller fPAR estimates than ground reference total fPAR. MODIS fPAR and Landsat 8 NDVI based fPAR estimates were similar to forest canopy fPAR.

  6. Parálisis de convergencia Convergence pals

    Directory of Open Access Journals (Sweden)

    Teresita de J Méndez Sánchez

    2010-01-01

    Full Text Available La parálisis de convergencia se caracteriza por la imposibilidad de la convergencia en la mirada próxima, con diplopía horizontal cruzada a partir de 1 metro, con aducción normal. La parálisis de la convergencia es una condición distinta de la insuficiencia de la convergencia y normalmente secundaria a una lesión intracraneal. Acude paciente de 12 años de edad por presentar diplopía horizontal de cerca ± 1 metro de 3 meses de evolución, cefalea, lagrimeo y fotofobia. La impresión diagnóstica fue parálisis de convergencia. En los estudios neurológicos solo se encontró implantación baja de las amígdalas cerebrales. Aún no se precisa la etiología aunque se recoge el antecedente de un traumatismo craneal sin relevancia por la familia.The convergence paralysis is characterized by impossible convergence in the near sight, with crossed horizontal diplopia at one meter distance and normal adduction. The convergence paralysis is a condition different from the inadequate convergence and usually secondary to intracranial lesion. A 12-years old patient went to the hospital because he had suffered horizontal diplopia in near sight (± 1 meter for 3 months, migraine, watering and photophobia. The definitive diagnosis was convergence paralysis. In the neurological studies, the low placing of the tonsils of cerebellum was the only finding. The etiology of this disorder is not determined yet; although there is some history of head trauma to which the family did not pay enough attention.

  7. Is PAR a Good Investment? Understanding the Costs and Benefits of Teacher Peer Assistance and Review Programs

    Science.gov (United States)

    Papay, John P.; Johnson, Susan Moore

    2012-01-01

    Peer Assistance and Review (PAR) is a local labor-management initiative designed to improve teacher quality. In PAR, expert "consulting teachers" mentor, support, and evaluate novice and underperforming veteran teachers. Evaluations under PAR can lead to dismissals. The authors examine the costs and benefits of PAR, both financial and…

  8. The PAR complex regulates pulsed actomyosin contractions during amnioserosa apical constriction in Drosophila.

    Science.gov (United States)

    David, Daryl J V; Tishkina, Alisa; Harris, Tony J C

    2010-05-01

    Apical constriction is a major mechanism underlying tissue internalization during development. This cell constriction typically requires actomyosin contractility. Thus, understanding apical constriction requires characterization of the mechanics and regulation of actomyosin assemblies. We have analyzed the relationship between myosin and the polarity regulators Par-6, aPKC and Bazooka (Par-3) (the PAR complex) during amnioserosa apical constriction at Drosophila dorsal closure. The PAR complex and myosin accumulate at the apical surface domain of amnioserosa cells at dorsal closure, the PAR complex forming a patch of puncta and myosin forming an associated network. Genetic interactions indicate that the PAR complex supports myosin activity during dorsal closure, as well as during other steps of embryogenesis. We find that actomyosin contractility in amnioserosa cells is based on the repeated assembly and disassembly of apical actomyosin networks, with each assembly event driving constriction of the apical domain. As the networks assemble they translocate across the apical patch of PAR proteins, which persist at the apical domain. Through loss- and gain-of-function studies, we find that different PAR complex components regulate distinct phases of the actomyosin assembly/disassembly cycle: Bazooka promotes the duration of actomyosin pulses and Par-6/aPKC promotes the lull time between pulses. These results identify the mechanics of actomyosin contractility that drive amnioserosa apical constriction and how specific steps of the contractile mechanism are regulated by the PAR complex.

  9. Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles.

    Science.gov (United States)

    Bharat, Tanmay A M; Murshudov, Garib N; Sachse, Carsten; Löwe, Jan

    2015-07-02

    Active segregation of Escherichia coli low-copy-number plasmid R1 involves formation of a bipolar spindle made of left-handed double-helical actin-like ParM filaments. ParR links the filaments with centromeric parC plasmid DNA, while facilitating the addition of subunits to ParM filaments. Growing ParMRC spindles push sister plasmids to the cell poles. Here, using modern electron cryomicroscopy methods, we investigate the structures and arrangements of ParM filaments in vitro and in cells, revealing at near-atomic resolution how subunits and filaments come together to produce the simplest known mitotic machinery. To understand the mechanism of dynamic instability, we determine structures of ParM filaments in different nucleotide states. The structure of filaments bound to the ATP analogue AMPPNP is determined at 4.3 Å resolution and refined. The ParM filament structure shows strong longitudinal interfaces and weaker lateral interactions. Also using electron cryomicroscopy, we reconstruct ParM doublets forming antiparallel spindles. Finally, with whole-cell electron cryotomography, we show that doublets are abundant in bacterial cells containing low-copy-number plasmids with the ParMRC locus, leading to an asynchronous model of R1 plasmid segregation.

  10. PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity

    Directory of Open Access Journals (Sweden)

    Henrique FUKUSHIMA

    Full Text Available Abstract Recent studies investigating protease-activated receptor type 2 (PAR-2 suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF of patients with moderate chronic periodontitis (MP. The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19 at baseline, and from MP patients (n = 19 and severe chronic periodontitis (SP (n = 19 patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR. PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p <0.05 PAR-2 expression in patients with periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection.

  11. GESTION "PAR" LES COMPETENCES ET POSITIONNEMENT DU CONTROLE DE GESTION ?

    OpenAIRE

    Rouby, Evelyne; Guy SOLLE

    2002-01-01

    International audience; Cet article a pour but de contribuer à une définition des contours du concept de "gestion par les compétences". De notre point de vue, ce concept relèverait des mêmes principes que la compétence organisationnelle à savoir les principes "systémique, de finalité, de spécificité, de visibilité et de reconnaissance, dynamique et cumulatif". Il consisterait à en assurer l'interaction dynamique entre les niveaux stratégiques et opérationnels et selon des cycles temporels dif...

  12. Caso práctico parálisis cerebral

    OpenAIRE

    Marcos Gómez, Sergio; Queimadelos Navarro, Sofía; Rodríguez Martín, Samuel; Sañudo Herrero, Alba

    2016-01-01

    El presente trabajo está enfocado al área psicomotor, siguiendo las bases de la intervención de Atención Temprana, desde el campo de la Terapia Ocupacional en la afectación de Parálisis Cerebral Infantil (PCI). El tratamiento empleado está dirigido a potenciar la funcionalidad del miembro afecto, para facilitar al paciente su desempeño en las Actividades de la Vida Diaria, Ocio y Tiempo Libre. Tras llevar a cabo la intervención, se realizó una recogida de datos y se analizó si los objet...

  13. INFECTION VIH REVELEE PAR UN ABCES UNILATERAL DU

    African Journals Online (AJOL)

    chez les sujets immunodéprimés en rapport avec l'épidémie du SIDA. Cet article rapporte le cas d'un patient dont l'infection à VIH a été révélée par un abcès primitif du psoas. OBSERVATION. Il s'agit d'un patient âgé de 25 ans, célibataire, artisan de profession à Marrakech, hétéro-sexuel, ayant eu des rapports sexuels.

  14. Analysis of clinical features and visual outcomes of pars planitis.

    Science.gov (United States)

    Berker, Nilufer; Sen, Emine; Elgin, Ufuk; Atilgan, Cemile Ucgul; Dursun, Erdem; Yilmazbas, Pelin

    2017-04-07

    To evaluate the demographic characteristics, clinical features, treatment and outcomes of patients with pars planitis in a tertiary referral center in Turkey. Medical records of patients with pars planitis were retrospectively reviewed. The data including demographic and ocular features and treatment outcomes were recorded. The distribution of clinical findings and complications were evaluated according to age and gender groups. The changes in final BCVA compared to the initial BCVA were noted. Statistical analysis was performed using SPSS software (Version 18.0, SPSS Inc., Chicago, USA). Twenty-seven patients (54 eyes) were included in this study. 16 patients were male (59.3%), and 11 were female (40.7%). Mean age at diagnosis was 12.84 ± 8.26 (range 4-36) years. Mean follow-up period was 61.3 ± 52.15 (range 9-172) months. Mean BCVA was 0.58 ± 0.36 (range 0.03-1.00) (0.40 ± 0.45 logMAR) at presentation, and 0.81 ± 0.28 (range 0.10-1.00) (0.14 ± 0.27 logMAR) at final visit (P = 0.001). Vitreous inflammation (100%), vitreous haze (92.6%), snowballs (74.1%), snowbanks (66.7%), anterior chamber cells (66.7%) and peripheral retinal vascular sheathing (48.1%) were the most common presentations. Ocular complications included vitreous condensation (51.9%), cystoid macular edema (22.2%), cataract (18.5%), inferior peripheral retinal detachment (11.1%), glaucoma (5.6%) and vitreous hemorrhage (3.7%). Treatments included topical, periocular, intravitreal and systemic corticosteroids, immunosuppressives, peripheral laser photocoagulation and pars plana vitrectomy when needed. Pars planitis is an idiopathic chronic intermediate uveitis mostly affecting children and adolescents. In spite of its chronic nature with high potential of causing ocular complications, adequate treatment and close follow-up lead to favorable visual outcomes.

  15. Cartographie par carroyage et précision spatiale

    Directory of Open Access Journals (Sweden)

    Patrice LANGLOIS

    1998-03-01

    Full Text Available Dans le cadre d'une cartographie par carroyage, on étudie la désagrégation de variables zonales, en revenant sur l'hypothèse de l'équirépartition spatiale des populations. L'objectif est d'approcher des règles différenciées de désagrégation des variables en fusionnant des plans d'information de nature morphologique, topologique et environnementale.

  16. Pollution atmospherique par emission de gaz d'echappement des ...

    African Journals Online (AJOL)

    La pollution atmosphérique a été pendant longtemps considérée comme un mal propre aux pauvres mais de nos jours, elle n'épargne ni pays africains, ni pays européens et devient un problème mondial de santé. L'objectif du présent travail est d'étudier la pollution atmosphérique engendrée par les gaz d'échappement ...

  17. La Manufacture d’Armes de Saint-Étienne : un patrimoine militaire saisi par l’économie créative

    Directory of Open Access Journals (Sweden)

    Thomas Zanetti

    2011-07-01

    Full Text Available Cet article prend pour objet d’analyse un projet de renouvellement urbain d’un espace hérité de l’activité industrielle et militaire : la Manufacture d’Armes de Saint-Étienne. L’histoire et les caractéristiques patrimoniales de ce site, à la fois dans leurs dimensions matérielles et immatérielles, sont mobilisées dans un projet urbain hautement stratégique, et donc soumis à de multiples enjeux : la Cité du Design de Saint-Étienne. Cet équipement s’insère quant à lui dans un cadre plus large de concurrence métropolitaine et de course à l’attractivité des territoires. En effet, dans une ville marquée par le traumatisme issu du déclin de l’industrie, l’existence d’un patrimoine militaire est conçue comme une opportunité de spécifier localement la mise en œuvre des « bonnes pratiques » urbaines en circulation dans les villes européennes. Les responsables de la Cité ont donc choisi un haut lieu de l’histoire industrielle et militaire locale pour accueillir le futur symbole de la modernité stéphanoise, dans le but de tirer de cette inscription patrimoniale une plus-value historique nécessaire à la réussite du projet dans un contexte de marketing urbain. Toutefois, le processus de patrimonialisation de la Manufacture d’Armes a donné naissance à un profond conflit opposant des associations locales de protection du patrimoine aux responsables de l’urbanisme. Il importe alors de questionner l’entreprise de valorisation du patrimoine militaire en analysant quels sont les héritages mémoriels associés à l’action publique urbaine contemporaine, voire instrumentés par sa stratégie de développement territorial.This article is an analysis of a project of urban renewal concerning a space inherited from industrial and military activity: the arms factory of Saint-Étienne. The history and the heritage characteristics of the site, in both its tangible and intangible dimensions, have been

  18. Populismo municipal y nacionalcatolicismo en la Valencia del general Primo de Rivera: el marqués de Sotelo (1923-1930 = Populism and Municipal Propaganda in Valencia during Primo de Rivera’s Dictatorship: the Marquis of Sotelo (1923-1930.

    Directory of Open Access Journals (Sweden)

    Julio López Iñíguez

    2016-05-01

    Full Text Available El presente trabajo pretende explicar las principales características políticas del régimen de Primo de Rivera en la ciudad de Valencia. Desde un análisis de la política municipal, el populismo y la publicidad de la construcción de obras públicas se presentan como los principales pilares para la consolidación del régimen en Valencia. La praxis política del marqués de Sotelo supuso para la ciudad el primer contacto con el nacionalcatolicismo. La figura de este alcalde, además, ocupa buena parte del texto al ser un fiel exponente de las prácticas políticas nacidas tras el golpe de septiembre de 1923.This paper aims to explain the main features of the political regime of Primo de Rivera in the city of Valencia. From an analysis of municipal politics, populism and advertising public works construction are presented as the main supports for the consolidation of the regime in Valencia. The political praxis of the Marquis of Sotelo assumed for the city’s first contact with the National Catholicism. The figure of the mayor, also occupies much of the text to be a faithful exponent of political practices born after the September 1923 coup.

  19. Plan estratégico de mercadeo “Un acto de fe por la valorización de la cultura prehispánica” para el Museo Arqueológico Casa del Marqués de San Jorge

    Directory of Open Access Journals (Sweden)

    Karem Lucenith Triviño Weaver

    2012-11-01

    Full Text Available Actualmente, los museos pueden ser clasificados en dos grupos: limitados en su objeto social, coartados en su misión, en sus programas y en sus públicos, faltos de imaginación, de estrategia y de gestión. Por otro lado, están los museos conscientes de la necesidad de competir por sobrevivir en el mercado, que aceptan combatir por el auto sostenimiento a través de la oferta de productos innovadores y apuntan a comunicar a un público, el mensaje correcto. A partir de un ejemplo específico, el Museo Arqueológico Casa del Marqués de San Jorge, el siguiente artículo prueba que es posible aplicar en estas instituciones una metodología de desarrollo de Plan Estratégico de Mercadeo (PEM, obteniendo un museo posicionado que hará parte de la oferta turística y cultural de la localidad de La Candelaria y de la ciudad de Bogotá.

  20. Apprentissage de Dictionnaires Multivariés et Décomposition Parcimonieuse Invariante par Translation et par Rotation 2D

    OpenAIRE

    Barthélemy, Quentin; Larue, Anthony; Mayoue, Aurélien; Mercier, David; MARS, Jerome

    2011-01-01

    National audience; Cet article présente le Multivariate Dictionary Learning Algorithm qui apprend en ligne les structures élémentaires associées à un ensemble de signaux multivariés. Une fois apprises, le Multivariate Orthogonal Matching Pursuit décompose tous les signaux de cet ensemble avec parcimonie. Ces méthodes sont spécifiées dans le cas d'invariance par rotation qui produit un dictionnaire restreint de noyaux. Nos méthodes sont appliquées à des données d'écriture manuscrite, afin d'ex...

  1. PhyloPars: estimation of missing parameter values using phylogeny.

    Science.gov (United States)

    Bruggeman, Jorn; Heringa, Jaap; Brandt, Bernd W

    2009-07-01

    A wealth of information on metabolic parameters of a species can be inferred from observations on species that are phylogenetically related. Phylogeny-based information can complement direct empirical evidence, and is particularly valuable if experiments on the species of interest are not feasible. The PhyloPars web server provides a statistically consistent method that combines an incomplete set of empirical observations with the species phylogeny to produce a complete set of parameter estimates for all species. It builds upon a state-of-the-art evolutionary model, extended with the ability to handle missing data. The resulting approach makes optimal use of all available information to produce estimates that can be an order of magnitude more accurate than ad-hoc alternatives. Uploading a phylogeny and incomplete feature matrix suffices to obtain estimates of all missing values, along with a measure of certainty. Real-time cross-validation provides further insight in the accuracy and bias expected for estimated values. The server allows for easy, efficient estimation of metabolic parameters, which can benefit a wide range of fields including systems biology and ecology. PhyloPars is available at: http://www.ibi.vu.nl/programs/phylopars/.

  2. Lumbar vertebral body and pars fractures following laminectomy.

    Science.gov (United States)

    Yadhati, Akshay; Kurra, Swamy; Tallarico, Richard A; Lavelle, William F

    2017-02-01

    A 56-year-old alcoholic male incurred L5 vertebral body and bilateral L4 pars fractures with progressive L4 on L5 anterolisthesis following low-energy falls while intoxicated. Recently, he had a L3-S1 laminectomy for lumbar spinal stenosis with claudication. Preoperative imaging and radiographs were negative for pars defects and instability, so an isolated decompressive surgery was performed. Following low-energy falls, his outpatient work-up revealed fractures through the bilateral L4 pedicles and posterior third of L5 vertebral body, with recurrence of axial back pain and bilateral lower extremity radiculopathy. He underwent revision decompression from L4-S1 and posterior instrumented fusion with transforaminal lumbar interbody fusion performed at each revised level. His axial back pain and radiculopathy improved postoperatively. Instability of a lumbar spine fracture pattern can be due to the remote or prior iatrogenic disruption of the posterior ligamentous complex. Our patient benefitted from surgery and his low back pain was resolved.

  3. Stress reactions involving the pars interarticularis in young athletes

    Energy Technology Data Exchange (ETDEWEB)

    Jackson, D.W.; Wiltse, L.L.; Dingeman, R.D.; Hayes, M.

    A stress reaction involving the pars interarticularis of the lumbar spine was confirmed in seven young athletes with a positive technetium pyrophosphate bone scan. No pars defects were detectable on their lumbosacral roentgenograms, which included oblique views. The return to normal levels of radioactive uptake on repeat bone scans correlated closely with their clinical course. If the bony reaction is recognized early, it may heal at a subroentgenographic level and prevent the development of lumbar spondylolysis. These early lesions usually show unilateral increased uptake at one lumbar level on the bone scan and, initially, the athlete localizes the pain to the corresponding unilateral lumbar paraspinous area. The ''one-legged hyperextension test'' is positive on the ipsilateral side and aggravates the pain. Treatment consists of avoiding the aggravating activities and resting. The average time for return to pain-free competition was 7.3 months. These developing defects may be the source of considerable prolonged disability in the young athlete, particularly if undiagnosed and untreated.

  4. Basic isoforms of Par o 1, the major allergen of Parietaria officinalis pollen.

    Science.gov (United States)

    Coscia, M R; Ruffilli, A; Oreste, U

    1995-11-01

    We describe a group of basic isoforms of Par o 1 (cumulatively referred to as Par o 1b), purified by anion-exchange chromatography. The allergenic activity of Par o 1b was compared with that of the acidic isoform (Par o 1a) by RAST inhibition. Par o 1b showed a cathodic mobility in crossed immunoelectrophoresis. It was found to be homogeneous in SDS-PAGE and SE-HPLC (14.5 kDa), and heterogeneous in PAG-IEF, yielding five IgE-binding bands with pI ranging between 7.9 and 9.6 PAG-IEF individual components were isolated by cation-exchange HPLC. The N-terminal amino acid sequence of the main component (pI 8.8) was determined and found to be similar to that of Par o 1a.

  5. Paracrine Apoptotic Effect of p53 Mediated by Tumor Suppressor Par-4

    Directory of Open Access Journals (Sweden)

    Ravshan Burikhanov

    2014-01-01

    Full Text Available The guardian of the genome, p53, is often mutated in cancer and may contribute to therapeutic resistance. Given that p53 is intact and functional in normal tissues, we harnessed its potential to inhibit the growth of p53-deficient cancer cells. Specific activation of p53 in normal fibroblasts selectively induced apoptosis in p53-deficient cancer cells. This paracrine effect was mediated by p53-dependent secretion of the tumor suppressor Par-4. Accordingly, the activation of p53 in normal mice, but not p53−/− or Par-4−/− mice, caused systemic elevation of Par-4, which induced apoptosis of p53-deficient tumor cells. Mechanistically, p53 induced Par-4 secretion by suppressing the expression of its binding partner, UACA, which sequesters Par-4. Thus, normal cells can be empowered by p53 activation to induce Par-4 secretion for the inhibition of therapy-resistant tumors.

  6. Réduction du risque d'infection par le VIH au Botswana - essais ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Au Botswana, ce sont les jeunes femmes qui sont le plus touchées par les nouvelles infections par le VIH. Le présent projet a pour but de réduire le nombre de nouveaux cas d'infection par le VIH, en particulier chez les femmes de 15 à 29 ans. Bien que l'on recense des cas de VIH/sida dans la plupart des régions du ...

  7. Traitement de la margine brute d'huile d'olive par distillation suivi de ...

    African Journals Online (AJOL)

    SARAH

    31 juil. 2014 ... Traitement de la margine brute d'huile d'olive par distillation suivi de neutralisation par chaux. 6867 ... Mots clés : margine d'huile d'olive, caractérisation, distillation, neutralisation. The treatment of olive oil mill .... a été déterminée selon la méthode standard (APHA 1992) par une oxydation de la matière.

  8. Alpine grassland fPAR change over the Northern Tibetan Plateau from 2002 to 2011

    Directory of Open Access Journals (Sweden)

    Bao-Xiong Chen

    2017-06-01

    Full Text Available In this study, two different methods including Digital Camera and Reference Panel (DCRP and traditional in situ fPAR observation for measuring the in situ point fPAR of very short alpine grass vegetation were compared, and the Moderate Resolution Imaging Spectroradiometer (MODIS fPAR products were evaluated and validated by in situ point data on the alpine grassland over the Northern Tibetan Plateau, which is sensitive to climate change and vulnerable to anthropogenic activities. Results showed that the MODIS alpine grassland fPAR product, examined by using DCRP, and traditional in situ fPAR observation had a significant relationship at the spatial and temporal scales. The decadal MODIS fPAR trend analysis showed that, average growing season fPAR increased by 1.2 × 10−4 per year and in total increased 0.86% from 2002 to 2011 in alpine grassland, when most of the fPAR increments occurred in southeast and center of the Northern Tibetan Plateau, the alpine grassland tended to recover from degradation slightly. However, climatic factors have influenced the various alpine grassland vegetation fPAR over a period of 10 years; precipitation significantly affected the alpine meadow fPAR in the eastern region, whereas temperature considerably influenced the alpine desert steppe fPAR in the west region. These findings suggest that the regional heterogeneity in alpine grassland fPAR results from various environmental factors, except for vegetation characteristics, such as canopy structure and leaf area.

  9. Urokinase-type plasminogen activator receptor (uPAR) as a promising new imaging target

    DEFF Research Database (Denmark)

    Persson, Morten; Kjaer, Andreas

    2013-01-01

    Urokinase-type plasminogen activator receptor (uPAR) has been shown to be of special importance during cancer invasion and metastasis. However, currently, tissue samples are needed for measurement of uPAR expression limiting the potential as a clinical routine. Therefore, non-invasive methods are...... will discuss the potential future clinical application for uPAR imaging as a new imaging biomarker....

  10. Magnetic activity and radial velocity filtering of young Suns: the weak-line T-Tauri stars Par 1379 and Par 2244

    Science.gov (United States)

    Hill, C. A.; Carmona, A.; Donati, J.-F.; Hussain, G. A. J.; Gregory, S. G.; Alencar, S. H. P.; Bouvier, J.; The Matysse Collaboration

    2017-12-01

    We report the results of our spectropolarimetric monitoring of the weak-line T-Tauri stars (wTTSs) Par 1379 and Par 2244, within the MaTYSSE (Magnetic Topologies of Young Stars and the Survival of close-in giant Exoplanets) programme. Both stars are of a similar mass (1.6 and 1.8 M⊙) and age (1.8 and 1.1 Myr), with Par 1379 hosting an evolved low-mass dusty circumstellar disc, and with Par 2244 showing evidence of a young debris disc. We detect profile distortions and Zeeman signatures in the unpolarized and circularly polarized lines for each star, and have modelled their rotational modulation using tomographic imaging, yielding brightness and magnetic maps. We find that Par 1379 harbours a weak (250 G), mostly poloidal field tilted 65° from the rotation axis. In contrast, Par 2244 hosts a stronger field (860 G) split 3:2 between poloidal and toroidal components, with most of the energy in higher order modes, and with the poloidal component tilted 45° from the rotation axis. Compared to the lower mass wTTSs, V819 Tau and V830 Tau, Par 2244 has a similar field strength, but is much more complex, whereas the much less complex field of Par 1379 is also much weaker than any other mapped wTTS. We find moderate surface differential rotation of 1.4× and 1.8× smaller than Solar, for Par 1379 and Par 2244, respectively. Using our tomographic maps to predict the activity-related radial velocity (RV) jitter, and filter it from the RV curves, we find RV residuals with dispersions of 0.017 and 0.086 km s-1 for Par 1379 and Par 2244, respectively. We find no evidence for close-in giant planets around either star, with 3σ upper limits of 0.56 and 3.54 MJup (at an orbital distance of 0.1 au).

  11. Comparative validation of the IPAQ and the 7-Day PAR among women diagnosed with breast cancer

    Directory of Open Access Journals (Sweden)

    Rock Cheryl L

    2006-03-01

    Full Text Available Abstract Background The criterion-related validity and measurement bias of the long form of the International Physical Activity Questionnaire (IPAQ was compared to the 7-Day Physical Activity Recall (PAR. Methods Participants were women who have been diagnosed with breast cancer and enrolled in the ongoing Women's Healthy Eating and Living Study. Women (N = 159, average age 57 years wore an accelerometer for one week and then completed the IPAQ or the PAR. Results The validity correlation of the PAR was significantly higher (p Conclusion The PAR was superior to the IPAQ in terms of validity, measurement bias, and screening statistics.

  12. Comparative validation of the IPAQ and the 7-Day PAR among women diagnosed with breast cancer

    Science.gov (United States)

    Johnson-Kozlow, Marilyn; Sallis, James F; Gilpin, Elizabeth A; Rock, Cheryl L; Pierce, John P

    2006-01-01

    Background The criterion-related validity and measurement bias of the long form of the International Physical Activity Questionnaire (IPAQ) was compared to the 7-Day Physical Activity Recall (PAR). Methods Participants were women who have been diagnosed with breast cancer and enrolled in the ongoing Women's Healthy Eating and Living Study. Women (N = 159, average age 57 years) wore an accelerometer for one week and then completed the IPAQ or the PAR. Results The validity correlation of the PAR was significantly higher (p ACSM physical activity guideline. Conclusion The PAR was superior to the IPAQ in terms of validity, measurement bias, and screening statistics. PMID:16579852

  13. Tryptase-PAR2 axis in experimental autoimmune prostatitis, a model for chronic pelvic pain syndrome.

    Science.gov (United States)

    Roman, Kenny; Done, Joseph D; Schaeffer, Anthony J; Murphy, Stephen F; Thumbikat, Praveen

    2014-07-01

    Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) affects up to 15% of the male population and is characterized by pelvic pain. Mast cells are implicated in the murine experimental autoimmune prostatitis (EAP) model as key to chronic pelvic pain development. The mast cell mediator tryptase-β and its cognate receptor protease-activated receptor 2 (PAR2) are involved in mediating pain in other visceral disease models. Prostatic secretions and urines from CP/CPPS patients were examined for the presence of mast cell degranulation products. Tryptase-β and PAR2 expression were examined in murine EAP. Pelvic pain and inflammation were assessed in the presence or absence of PAR2 expression and upon PAR2 neutralization. Tryptase-β and carboxypeptidase A3 were elevated in CP/CPPS compared to healthy volunteers. Tryptase-β was capable of inducing pelvic pain and was increased in EAP along with its receptor PAR2. PAR2 was required for the development of chronic pelvic pain in EAP. PAR2 signaling in dorsal root ganglia led to extracellular signal-regulated kinase (ERK)1/2 phosphorylation and calcium influx. PAR2 neutralization using antibodies attenuated chronic pelvic pain in EAP. The tryptase-PAR2 axis is an important mediator of pelvic pain in EAP and may play a role in the pathogenesis of CP/CPPS. Copyright © 2014 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

  14. Aberrations in pseudoautosomal regions (PARs) found in infertile men with Y-chromosome microdeletions.

    Science.gov (United States)

    Jorgez, Carolina J; Weedin, John W; Sahin, Aysegul; Tannour-Louet, Mounia; Han, Shuo; Bournat, Juan C; Mielnik, Anna; Cheung, Sau Wai; Nangia, Ajay K; Schlegel, Peter N; Lipshultz, Larry I; Lamb, Dolores J

    2011-04-01

    The pseudoautosomal regions (PARs) of the Y-chromosome undergo meiotic recombination with the X-chromosome. PAR mutations are associated with infertility and mental and stature disorders. The aim of the study was to determine whether men with Y-chromosome microdeletions have structural defects in PARs. Eighty-seven infertile men with Y-chromosome microdeletions and 35 controls were evaluated for chromosomal rearrangements using commercial or custom (X- and Y-chromosome) array comparative genomic hybridization or by quantitative PCR of selected PAR genes. Multisoftware-defined chromosomal gains or losses were validated by quantitative PCR and FISH. Array comparative genomic hybridization confirmed the AZF deletions identified by multiplex PCR. All men with Y-chromosome microdeletions and an abnormal karyotype displayed PAR abnormalities, as did 10% of men with Y-chromosome microdeletions and a normal karyotype. None of the control subjects or infertile men without Y-chromosome microdeletions had PAR duplications or deletions. SHOX aberrations occurred in 14 men (nine gains and five losses); four were short in stature (95th percentile). In contrast, the height of 23 men with Y-chromosome microdeletions and normal PARs was average at 176.8 cm (50th percentile). Y-chromosome microdeletions can include PAR defects causing genomic disorders such as SHOX, which may be transmitted to offspring. Previously unrecognized PAR gains and losses in men with Y-chromosome microdeletions may have consequences for offspring.

  15. The urokinase receptor (uPAR) facilitates clearance of Borrelia burgdorferi.

    OpenAIRE

    Hovius, Joppe W. R; Bijlsma, Maarten F.; van der Windt, Gerritje J.W.; W Joost Wiersinga; Bastiaan J D Boukens; Jeroen Coumou; Anneke Oei; Regina de Beer; de Vos, Alex F; Veer, Cornelis van 't; van Dam, Alje P.; Penghua Wang; Erol Fikrig; Levi, Marcel M; Roelofs, Joris J. T. H.

    2009-01-01

    The causative agent of Lyme borreliosis, the spirochete Borrelia burgdorferi, has been shown to induce expression of the urokinase receptor (uPAR); however, the role of uPAR in the immune response against Borrelia has never been investigated. uPAR not only acts as a proteinase receptor, but can also, dependently or independently of ligation to uPA, directly affect leukocyte function. We here demonstrate that uPAR is upregulated on murine and human leukocytes upon exposure to B. burgdorferi bo...

  16. Le cinéma mexicain par ses écoles

    OpenAIRE

    Lépine, Cédric

    2016-01-01

    Depuis le début des années 2000, le cinéma mexicain a fait preuve d’une formidable renaissance. Parmi les initiateurs de ce mouvement, on trouve à Mexico deux écoles de cinéma réputées dans toute l’Amérique latine : le CCC et le CUEC. En effet, des cinéastes qui ont marqué la récente actualité du septième art comme Francisco Vargas, Rodrigo Plá, Elisa Miller, Alfonso Cuarón, Fernando Eimbcke… en sont d’anciens élèves. Apparues dans les années 1960 et 1970 pour sortir le cinéma mexicain d’un c...

  17. Un « gouvernement des journalistes par le Pouvoir politique » par défaut ?

    Directory of Open Access Journals (Sweden)

    Matthieu Lardeau

    2013-12-01

    Full Text Available Cet article présente les interactions de deux mouvements concomitants apparusprincipalement dans les décennies 1950 à 1970 dans le champ de la presse française : d’un côté les initiatives – consécutives à la démarche pionnière, en 1951, de création de la première Société des journalistes (SDJ au sein du Monde – menées par les journalistes de rédactions de quotidiens pour créer des SDJ dont la destination peut aller jusqu’à gouverner les journaux; de l’autre côté, les « réponses » apportées par les Pouvoirs politique et public pour contenir ces initiatives visant à étendre le pouvoir des journalistes dans la gestion et la gouvernance de leurs journaux. Cette étude exploratoire repose principalement sur l’analyse de deux types de littérature, souvent déconsidérés ou négligés par le champ académique, en dépit de leur grande richesse : (1 la littérature professionnelle, ayant pour principaux auteurs les journalistes et managers de presse (Périer Daville, Boegner, Pigasse, etc., florissante depuis 1944 et singulièrement durant les décennies 1960 et 1970 ; (2 la littérature grise constituée notamment par des rapports publics rédigés par des institutions comme les services du Premier ministre (commissions Lindon de 1970 et Serisé de 1972, le Conseil économique et social, etc. Nos principaux résultats montrent que les propositions ou actions menées par les journalistes (1 restent le plus souvent inexploitées in concreto par les journalistes eux-mêmes; (2 entrent le plus souvent en opposition avec les politiques de nouveaux actionnaires ou directions de journaux et avec les projets des autorités politiques et publiques qui cherchent à encadrer l’autonomie de la profession journalistique pour mieux gouverner celle-ci ; (3 combien in fine les représentants des différents acteurs impliqués s’accordent pour contenir l’émergence d’un « gouvernement des journalistes » par les

  18. Stereotipi par dzimumu lomu sadali studentu ģimenē

    OpenAIRE

    Vilne, Līva

    2007-01-01

    Bakalaura darba tēma ir „Stereotipi par dzimumu lomu sadali studentu ģimenē”. Darbā tiek apskatītas dzimumu atšķirības, ģimenes skaidrojums un studentu ģimenes raksturojums, kā arī dzimumu lomu sadale studentu ģimenē un ar to saistītie stereotipi. Iegūtie dati liecina, ka studentu ģimenē valdošie sterotipi atbilst sabiedrībā pieņemtajiem tradicionālajiem stereotipiem. Jo dzimumu lomu sadale atbilst priekšstatiem – vīrietis nodrošina ģimeni finasiāli, bet sieviete ir mājsaimniece. Bakalaura da...

  19. IMPROVEMENT OF PRODUCTION PLANNING IN COMPANY PARS KOMPONENTY

    Directory of Open Access Journals (Sweden)

    René Klega

    2014-03-01

    Full Text Available The article analyzes the current status of production planning in company Pars Komponenty s.r.o., proposes a new method of planning based on application of the principle of MRP. It is a discrete type of production with high complexity of BOM and MTO (Make-to-Order and ETO (Engineering-to-Order from the point of decoupling point. The original planning system plans according to production capacity backward without collisions, but for a given type of production does not work in practice. Planning system was analyzed and the main problems were identified, which were high work in progress and material stocks. This article target is to propose a new planining system based on the inclusion of time reserves of purchased material items. New planning system was tested in practise with benefit in reducing both the material inventory and work in progress.

  20. Parálisis cerebral. Tratamiento ortopodológico

    OpenAIRE

    Querol Martínez, Esther; Crespo Martínez, Artur; Concustell Gonfaus, Josep; Sacristan Valero, Sergi; Concustell Fargas, Olga; Carrera Casanova, Ana

    2005-01-01

    Después de varios años de experiencia en la aplicación de las férulas estabilizadoras del sistema aquileocalcáneo plantar FESAP) en pacientes con parálisis cerebral, creemos que deberían ser el tratamiento ortopodológico de elección del pie equino en niños con miopatías y lesiones neurológlcas de la unidad motora, del sistema piramidal o extrapiramidal, tanto por su perfecta adaptación a la morfología de la extremidad inferior como por sus caracteristicas funcionales y sus acciones fisio1ógic...

  1. The polarity protein Par6 is coupled to the microtubule network during molluscan early embryogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Homma, Taihei [Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan); Shimizu, Miho [Kuroda Chiromorphology Team, ERATO-SORST, JST, Komaba, Meguro-ku, Tokyo 153-8902 (Japan); Kuroda, Reiko, E-mail: ckuroda@mail.ecc.u-tokyo.ac.jp [Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan); Kuroda Chiromorphology Team, ERATO-SORST, JST, Komaba, Meguro-ku, Tokyo 153-8902 (Japan); Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902 (Japan)

    2011-01-07

    Research highlights: {yields} The cDNAs encoding Par6 and aPKC homologues were cloned from the snail Lymnaea stagnalis. {yields} L. stagnalis Par6 directly interacts with tubulin and microtubules and localizes to the microtubule cytoskeleton during the early embryogenesis. {yields} Identical sequence and localization of LsPar6 for the dextral and the sinistral snails exclude the possibility of the gene being the primary determinant of body handedness. -- Abstract: Cell polarity, which directs the orientation of asymmetric cell division and segregation of fate determinants, is a fundamental feature of development and differentiation. Regulators of polarity have been extensively studied, and the critical importance of the Par (partitioning-defective) complex as the polarity machinery is now recognized in a wide range of eukaryotic systems. The Par polarity module is evolutionarily conserved, but its mechanism and cooperating factors vary among different systems. Here we describe the cloning and characterization of a pond snail Lymnaea stagnalis homologue of partitioning-defective 6 (Lspar6). The protein product LsPar6 shows high affinity for microtubules and localizes to the mitotic apparatus during embryonic cell division. In vitro assays revealed direct binding of LsPar6 to tubulin and microtubules, which is the first evidence of the direct interaction between the two proteins. The interaction is mediated by two distinct regions of LsPar6 both located in the N-terminal half. Atypical PKC, a functional partner of Par6, was also found to localize to the mitotic spindle. These results suggest that the L. stagnalis Par complex employs the microtubule network in cell polarity processes during the early embryogenesis. Identical sequence and localization of LsPar6 for the dextral and the sinistral snails exclude the possibility of the gene being the primary determinant of handedness.

  2. Paternal age related schizophrenia (PARS): Latent subgroups detected by k-means clustering analysis.

    Science.gov (United States)

    Lee, Hyejoo; Malaspina, Dolores; Ahn, Hongshik; Perrin, Mary; Opler, Mark G; Kleinhaus, Karine; Harlap, Susan; Goetz, Raymond; Antonius, Daniel

    2011-05-01

    Paternal age related schizophrenia (PARS) has been proposed as a subgroup of schizophrenia with distinct etiology, pathophysiology and symptoms. This study uses a k-means clustering analysis approach to generate hypotheses about differences between PARS and other cases of schizophrenia. We studied PARS (operationally defined as not having any family history of schizophrenia among first and second-degree relatives and fathers' age at birth ≥ 35 years) in a series of schizophrenia cases recruited from a research unit. Data were available on demographic variables, symptoms (Positive and Negative Syndrome Scale; PANSS), cognitive tests (Wechsler Adult Intelligence Scale-Revised; WAIS-R) and olfaction (University of Pennsylvania Smell Identification Test; UPSIT). We conducted a series of k-means clustering analyses to identify clusters of cases containing high concentrations of PARS. Two analyses generated clusters with high concentrations of PARS cases. The first analysis (N=136; PARS=34) revealed a cluster containing 83% PARS cases, in which the patients showed a significant discrepancy between verbal and performance intelligence. The mean paternal and maternal ages were 41 and 33, respectively. The second analysis (N=123; PARS=30) revealed a cluster containing 71% PARS cases, of which 93% were females; the mean age of onset of psychosis, at 17.2, was significantly early. These results strengthen the evidence that PARS cases differ from other patients with schizophrenia. Hypothesis-generating findings suggest that features of PARS may include a discrepancy between verbal and performance intelligence, and in females, an early age of onset. These findings provide a rationale for separating these phenotypes from others in future clinical, genetic and pathophysiologic studies of schizophrenia and in considering responses to treatment. Copyright © 2011 Elsevier B.V. All rights reserved.

  3. Clinical outcomes of pars plicata anterior vitrectomy: 2-year results

    Directory of Open Access Journals (Sweden)

    Priya Narang

    2015-01-01

    Full Text Available Purpose: To demonstrate the safety and outcome of a surgical approach that uses pars plicata site for anterior vitrectomy during phacoemulsification procedure complicated by posterior capsule rupture and residual cortical matter. Design: Single center, retrospective, interventional, noncomparative study. Materials and Methods: Medical records of a consecutive series of 35 eyes of 35 patients who underwent pars plicata anterior vitrectomy (PPAV were reviewed. The main outcome measures were corrected and uncorrected distance visual acuity (CDVA, UDVA, early and late postoperative complications and intraocular pressure (IOP. Ultrasound biomicroscopic (UBM evaluation of sclerotomy site and spectral domain optical coherence tomography analysis for central macular thickness (CMT was performed. The final visual outcome at 2 years was evaluated. Results: At 2 years follow-up, the mean postoperative UDVA (logarithm of the minimum angle of resolution [logMAR] and CDVA (logMAR was 0.49 ± 0.26 and 0.19 ± 0.14, respectively. There was no significant change in the IOP (P = 0.061 and the mean CMT at 2 years was 192.5 ± 5.54 mm. The postoperative UBM image of the sclerotomy site at 8 weeks demonstrated a clear wound without any vitreous adhesion or incarceration. Intraoperative hyphema was seen in 1 (2.8% case and postoperative uveitis was seen in 2 (5.7% cases, which resolved with medications. No case of an iatrogenic retinal break or retinal detachment was reported. Conclusions: PPAV enables a closed chamber approach, allows thorough cleanup of vitreous in the pupillary plane and anterior chamber and affords better access to the subincisional and retropupillary cortical remnant with a significant visual outcome and an acceptable complication rate.

  4. Correlation between Olea europaea and Parietaria judaica pollen counts and quantification of their major allergens Ole e 1 and Par j 1-Par j 2.

    Science.gov (United States)

    Moreno-Grau, Stella; Elvira-Rendueles, Belén; Moreno, José; García-Sánchez, Antonio; Vergara, Nuria; Asturias, Juan A; Arilla, M C; Ibarrola, Ignacio; Seoane-Camba, Juan A; Suárez-Cervera, Maria

    2006-06-01

    In patients with pollinosis, allergic symptoms are often correlated with the number of airborne pollen grains, although this correlation is not always close. The direct measurement of the concentration of aeroallergens has only recently been introduced and is an important advance in public health information systems. To compare specific quantification of aeroallergens Ole e 1 and Par j 1-Par j 2 Olea and Urticaceae pollen counts. The Hirst method sampler and the Burkard Cyclone sampler were used for pollen count and allergen quantification, respectively. The aerosol was extracted and quantified for Ole e 1 and Par j 1-Par j 2 content using enzyme-linked immunosorbent assay procedures. Day-to-day variations were observed in both the pollen count and the amount of allergens. Pollen counts and aeroallergen quantification were closely correlated with 99% significance (Olea/Ole e 1: R = 0.892, P < .001; Urticaceae/Par j 1-Par j 2: R = 0.734, P < .001). The technique for the sampling and quantification of aeroallergens presented in this article, based on enzyme-linked immunosorbent assay and applied to the protein extracts directly obtained from the bioaerosol, represents an important advance in the epidemiologic study of allergic respiratory diseases.

  5. A Bipolar Spindle of Antiparallel ParM Filaments Drives Bacterial Plasmid Segregation

    DEFF Research Database (Denmark)

    Gayathri, P; Fujii, T; Møller-Jensen, Jakob

    2012-01-01

    To ensure their stable inheritance by daughter cells during cell division, bacterial low copy-number plasmids make simple DNA segregating machines that use an elongating protein filament between sister plasmids. In the ParMRC system of Escherichia coli R1 plasmid, ParM, an actin-like protein, forms...

  6. Contamination des eaux souterraines par le lixiviat des décharges ...

    African Journals Online (AJOL)

    La percolation du lixiviat en provenance des dechets des decharges constitue une source de pollution des ressources en eau souterraine par infiltration, constituant ... un modele type de deterioration des ressources en eaux par un effet de contamination du lixiviat en provenance de la decharge et des pratiques agricoles.

  7. 177-lu labeled peptide for site-specific uPAR-targeting

    DEFF Research Database (Denmark)

    2015-01-01

    There is provided a 177-Lu labelled peptide for site-specific targeting of the Urokinase Plasminogen Activator Receptor (uPAR) thereby enabling treatment of a cancer disease associated with high uPAR expression; e.g. treatment of colorectal cancer by administering to a patient an effective amount...... of the 177-Lu labelled peptide....

  8. Effect of pre-drying and par-frying conditions on the crispness of French fries

    NARCIS (Netherlands)

    Loon, W.A.M.; Visser, J.E.; Linssen, J.P.H.; Somsen, D.J.; Klok, H.J.; Voragen, A.G.J.

    2007-01-01

    An experimental design was used to study the effect of pre-drying (to 10, 15 and 20% weight loss) and par-frying conditions (160, 170 and 180 °C) on the crispness of French fries. Par-frying time was adjusted with a software program to obtain equal moisture content and internal texture for all

  9. Participatory Action Research (PAR) cum Action Research (AR) in Teacher Professional Development: A Literature Review

    Science.gov (United States)

    Morales, Marie Paz E.

    2016-01-01

    This paper reviews Participatory Action Research as an approach to teacher professional development. It maps the origins of Participatory Action Research (PAR) and discusses the benefits and challenges that have been identified by other researchers in utilizing PAR approaches in conducting research. It draws ideas of combining the features of…

  10. Soluble urokinase plasminogen activator receptor (suPAR) in acute care

    DEFF Research Database (Denmark)

    Rasmussen, Line Jee Hartmann; Ladelund, Steen; Haupt, Thomas Huneck

    2016-01-01

    OBJECTIVE: Soluble urokinase plasminogen activator receptor (suPAR) is an inflammatory biomarker associated with presence and progression of disease and with increased risk of mortality. We aimed to evaluate the unspecific biomarker suPAR as a prognostic marker in patients admitted to acute care...

  11. Narrative Identities and the Plebiscite in Pará: An Analysis of the Front Pages for O Liberal and Diário do Pará

    Directory of Open Access Journals (Sweden)

    Alda Cristina Silva da Costa

    2017-05-01

    Full Text Available On December 11, 2011, a plebiscite was held in Pará proposing to create two separatist states, Carajás and Tapajós, out of the state of Pará. The public voted against both projects. This article analyzes the narrative identities found on the front pages of O Liberal and Diário do Pará newspapers about the plebiscite in Pará. Depth Hermeneutics (DH, as proposed by Thompson (2011, was used as the methodological reference. DH emphasizes the object of analysis as a meaningful symbolic construction requiring interpretation. Narrative analysis, as proposed by Motta (2007, was the main research technique used to highlight the movement of construction of journalistic (discursive characters. The narrative identities built by the two newspapers showed that both were against the creation of the new states, but for very different reasons. The flag of Pará was the main object used to induce the idea of unity. Em 11 de dezembro de 2011, o plebiscito no Pará propôs a criação dos estados de Carajás e de Tapajós a partir da divisão do estado do Pará. O resultado da consulta pública foi negativo aos dois projetos. O artigo analisa as identidades narrativas constituídas pelas primeiras páginas dos jornais O Liberal e Diário do Pará sobre o plebiscito no Pará. Utilizou-se como referencial metodológico a Hermenêutica em Profundidade (HP, proposta por Thompson (2011. A HP evidencia o fato de que o objeto de análise é uma construção simbólica significativa, que exige uma interpretação. Como principal técnica de pesquisa, a análise narrativa, proposta por Motta (2007, com ênfase no movimento de construção de personagens jornalísticas (discursivas. As identidades narrativas construídas pelos dois jornais indicaram que ambos eram contrários à criação dos novos estados, porém por motivos divergentes. A bandeira do Pará foi o principal elemento utilizado para evocar a ideia de unidade. En 11 de diciembre de 2011, el plebiscito en

  12. Activation of protease-activated receptors (PARs)-1 and -2 promotes alpha-smooth muscle actin expression and release of cytokines from human lung fibroblasts.

    Science.gov (United States)

    Asokananthan, Nithiananthan; Lan, Rommel S; Graham, Peter T; Bakker, Anthony J; Tokanović, Ana; Stewart, Geoffrey A

    2015-02-01

    Previous studies have shown that protease-activated receptors (PARs) play an important role in various physiological processes. In the present investigation, we determined the expression of PARs on human lung fibroblasts (HLF-1) and whether they were involved in cellular differentiation and pro-inflammatory cytokine and prostaglandin (PGE2) secretion. PAR-1, PAR-2, PAR-3, and PAR-4 were detected in fibroblasts using RT-PCR, immunocytochemistry, and flow cytometry. Increased expression of PAR-4, but not other PARs, was observed in fibroblasts stimulated with phorbol myristate acetate. The archetypical activators of PARs, namely, thrombin and trypsin, as well as PAR-1 and PAR-2 agonist peptides, stimulated transient increases in intracellular Ca(2+), and promoted increased α-smooth muscle actin expression. The proteolytic and peptidic PAR activators also stimulated the release of IL-6 and IL-8, as well as PGE2, with a rank order of potency of PAR-1 > PAR-2. The combined stimulation of PAR-1 and PAR-2 resulted in an additive release of both IL-6 and IL-8. In contrast, PAR-3 and PAR-4 agonist peptides, as well as all the PAR control peptides examined, were inactive. These results suggest an important role for PARs associated with fibroblasts in the modulation of inflammation and remodeling in the airway. © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

  13. Attic cholesteatoma with closure of the entrance to pars flaccida retraction pocket.

    Science.gov (United States)

    Matsuzawa, Shingo; Iino, Yukiko; Yamamoto, Daiki; Hasegawa, Masayo; Hara, Mariko; Shinnabe, Akihiro; Kanazawa, Hiromi; Yoshida, Naohiro

    2017-12-01

    We report three patients with pars flaccida-type cholesteatoma (attic cholesteatoma) with closure of the entrance to the cholesteatoma at the time of surgery. These patients were diagnosed with attic cholesteatoma requiring surgery on the basis of abnormal findings of the pars flaccida, audiometry, and temporal bone computed tomography during the clinical course. Intraoperatively, cholesteatoma matrix and granulation tissue were observed behind the intact pars flaccida epithelium, which suggested that the entrance had apparently closed and the continuity with the cholesteatoma matrix disappeared after resolution of inflammation at the pars flaccida. In such patients, a normal pars flaccida may cause cholesteatoma to be initially overlooked, or misdiagnosed as congenital cholesteatoma. The diagnosis should be carefully made on the basis of the clinical course and the results of various examinations. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Compression Myelopathy due to Proliferative Changes around C2 Pars Defects without Instability.

    Science.gov (United States)

    Kimura, Tetsuya; Sakai, Toshinori; Tezuka, Fumitake; Abe, Mitsunobu; Yamashita, Kazuta; Takata, Yoichiro; Higashino, Kosaku; Sairyo, Koichi

    2016-06-01

    We report a case with compression myelopathy due to proliferative changes around the C2 pars defects without instability. A 69-year-old man presented with progressive clumsy hands and spastic gait. Plain radiographs showed bilateral spondylolysis (pars defects) at C2 and fusion between C2 and C3 spinous processes. Dynamic views revealed mobility through the pars defects, but there was no apparent instability. Computed tomography showed proliferative changes at the pars defects, which protruded into spinal canal. On magnetic resonance imaging, the spinal cord was compressed and intramedullary high signal change was found. A diagnosis of compression myelopathy due to proliferative changes around the C2 pars defects was made. We performed posterior decompression. Postoperatively, symptoms have been alleviated and images revealed sufficient decompression and no apparent instability. In patients with the cervical spondylolysis, myelopathy caused by instability or slippage have been periodically reported. The present case involving C2 spondylolysis is extremely rare.

  15. Accuracy and efficacy of fluoroscopy-guided pars interarticularis injections on immediate and short-term pain relief.

    Science.gov (United States)

    Kershen, L Michael; Nacey, Nicholas C; Patrie, James T; Fox, Michael G

    2016-10-01

    To determine the accuracy and short-term efficacy of fluoroscopy-guided steroid/anesthetic injections for symptomatic pars interarticularis (pars) defects. Following IRB approval, all fluoroscopically guided pars injections from a single institution (6/2010 to 3/2016) were retrospectively and independently reviewed by two MSK radiologists. The radiologists evaluated the fluoroscopic images to determine if all of the pars injections associated with each procedure were intra-pars (n = 57 procedures; 106 pars injections), peri-pars (n = 3 procedures; three pars injected), or a combination of intra-pars and peri-pars (n = 6 procedures; 12 pars injected). The patients were asked their pain score (graded on a scale of 0-10) pre-injection, 5-10 min and 1-week post-injection. Age, gender, and fluoroscopic times were recorded. Statistical analysis was performed on the all intra-pars injections only. Exact inter-reader agreement was present in 92 % (112/121) of the injections, with 57 of the procedures (106 pars injections) performed on 41 patients (mean age 36; 18 M, 23 F) all intra-pars. The mean pre-injection and 5-10 min post-injection reduction in pain for the all intra-pars injections was -3.0 units (95 % CI: [-3.9, -2.1] units; p pars) reduction in pain of -0.7 units (95 % CI [-1.5, 0.0]; p = 0.06). The geometric mean fluoroscopic time per pars injected was 42 s. Over 92 % of fluoroscopically guided injections for symptomatic spondylolysis are technically successful with minimum fluoroscopic time, resulting in statistically significant pain reduction immediately post-injection and a trend in pain reduction 1-week post-injection.

  16. Par-1b is required for morphogenesis and differentiation of myoepithelial cells during salivary gland development.

    Science.gov (United States)

    Gervais, Elise M; Sequeira, Sharon J; Wang, Weihao; Abraham, Stanley; Kim, Janice H; Leonard, Daniel; DeSantis, Kara A; Larsen, Melinda

    2016-10-01

    The salivary epithelium initiates as a solid mass of epithelial cells that are organized into a primary bud that undergoes morphogenesis and differentiation to yield bilayered acini consisting of interior secretory acinar cells that are surrounded by contractile myoepithelial cells in mature salivary glands. How the primary bud transitions into acini has not been previously documented. We document here that the outer epithelial cells subsequently undergo a vertical compression as they express smooth muscle α-actin and differentiate into myoepithelial cells. The outermost layer of polarized epithelial cells assemble and organize the basal deposition of basement membrane, which requires basal positioning of the polarity protein, Par-1b. Whether Par-1b is required for the vertical compression and differentiation of the myoepithelial cells is unknown. Following manipulation of Par-1b in salivary gland organ explants, Par-1b-inhibited explants showed both a reduced vertical compression of differentiating myoepithelial cells and reduced levels of smooth muscle α-actin. Rac1 knockdown and inhibition of Rac GTPase function also inhibited branching morphogenesis. Since Rac regulates cellular morphology, we investigated a contribution for Rac in myoepithelial cell differentiation. Inhibition of Rac GTPase activity showed a similar reduction in vertical compression and smooth muscle α-actin levels while decreasing the levels of Par-1b protein and altering its basal localization in the outer cells. Inhibition of ROCK, which is required for basal positioning of Par-1b, resulted in mislocalization of Par-1b and loss of vertical cellular compression, but did not significantly alter levels of smooth muscle α-actin in these cells. Overexpression of Par-1b in the presence of Rac inhibition restored basement membrane protein levels and localization. Our results indicate that the basal localization of Par-1b in the outer epithelial cells is required for myoepithelial cell

  17. Inhibition of uPAR-TGFβ crosstalk blocks MSC-dependent EMT in melanoma cells.

    Science.gov (United States)

    Laurenzana, Anna; Biagioni, Alessio; Bianchini, Francesca; Peppicelli, Silvia; Chillà, Anastasia; Margheri, Francesca; Luciani, Cristina; Pimpinelli, Nicola; Del Rosso, Mario; Calorini, Lido; Fibbi, Gabriella

    2015-07-01

    The capacity of cancer cells to undergo epithelial-to-mesenchymal transition (EMT) is now considered a hallmark of tumor progression, and it is known that interactions between cancer cells and mesenchymal stem cells (MSCs) of tumor microenvironment may promote this program. Herein, we demonstrate that MSC-conditioned medium (MSC-CM) is a potent inducer of EMT in melanoma cells. The EMT profile acquired by MSC-CM-exposed melanoma cells is characterized by an enhanced level of mesenchymal markers, including TGFβ/TGFβ-receptors system upregulation, by increased invasiveness and uPAR expression, and in vivo tumor growth. Silencing TGFβ in MSC is found to abrogate ability of MSC to promote EMT characteristics in melanoma cells, together with uPAR expression, and this finding is strengthened using an antagonist peptide of TGFβRIII, the so-called P17. Finally, we demonstrate that the uPAR antisense oligonucleotide (uPAR aODN) may inhibit EMT of melanoma cells either stimulated by exogenous TGFβ or MSC-CM. Thus, uPAR upregulation in melanoma cells exposed to MSC-medium drives TGFβ-mediated EMT. On the whole, TGFβ/uPAR dangerous liaison in cancer cell/MSC interactions may disclose a new strategy to abrogate melanoma progression. Mesenchymal stem cell (MSC)-conditioned medium induces EMT-like profile in melanoma. MSC-derived TGFβ promotes uPAR and TGFβ/TGFβ-receptor upregulation in melanoma. TGFβ gene silencing in MSCs downregulates uPAR expression and EMT in melanoma. uPAR downregulation prevents MSC-induced EMT-like profile in melanoma cells. Inhibition of the dangerous TGFβ/uPAR relationship might abrogate melanoma progression.

  18. Facteurs de risque de mortalité par tuberculose pulmonaire

    Science.gov (United States)

    Janah, Hicham; Souhi, Hicham; Kouismi, Hatim; Mark, Karima; Zahraoui, Rachida; Benamor, Jouda; Soualhi, Mona; Bourkadi, Jamal Eddine

    2014-01-01

    La tuberculose est une maladie infectieuse transmissible provoquée par myco-bacterium tuberculosis (bacille de Koch ou BK). Elle représente, selon les estimations del'Organisation Mondiale de la Santé (OMS), l'une des pathologies infectieuses causant le plus de décès au niveau mondial avec plus de 1 million de décès par an. Pour déterminer les facteurs de risque de mortalité au cours de la tuberculose pulmonaire à microscopie positive nous avons mené une étude rétrospective portant sur tous les cas de tuberculose pulmonaire à microscopie positive et qui étaient décédés au cours de leur hospitalisation. Cette étude a colligé 1803 cas de tuberculose sur une période de 2 ans et demi dont 46 sont décédés. La prévalence de décès est de 2,55%. La population se répartit en 32 hommes et 14 femmes. L’âge moyen était de 53ans ± 17 ans. Le tabagisme était retrouvé chez la moitié des cas. Une comorbidité était retrouvée dans 43%, avec 17% de diabète. Le délai de diagnostic avait une médiane de 60 jours avec percentile (30j; 105j). La symptomatologie clinique était dominée par la toux, la dyspnée et les expectorations soit respectivement: 97,8%, 69,6% et 67,4% des cas. Sur le plan radiologique les lésions étaient diffuses et bilatérales dans 76,1% des cas. Tous les patients étaient mis sous SRHZ. 11% avaient présenté une toxicité aux antibacillaires (de type hépatiques dans 3 cas et neurologiques dans 2 cas). Le délai médian de décès était de 8,5 jours (5j; 17j). Les causes de décès retrouvées étaient: Une hépatite fulminante (3 cas), une décompensation acido-cétosique (3 cas), un SDRA (2 cas), des hémoptysies foudroyantes (2 cas), et respectivement un cas secondaire à une décompensation de BPCO, une décompensation cardiaque, une hypoglycémie et un tableau d'anasarque. Cette étude suggère que le terrain, le retard diagnostique et les effets secondaires du traitement sont les principaux facteurs de risque de

  19. Evaluation de la qualite osseuse par les ondes guidees ultrasonores =

    Science.gov (United States)

    Abid, Alexandre

    La caracterisation des proprietes mecaniques de l'os cortical est un domaine d'interet pour la recherche orthopedique. En effet, cette caracterisation peut apporter des informations primordiales pour determiner le risque de fracture, la presence de microfractures ou encore depister l'osteoporose. Les deux principales techniques actuelles de caracterisation de ces proprietes sont le Dual-energy X-ray Absorptiometry (DXA) et le Quantitative Computed Tomogaphy (QCT). Ces techniques ne sont pas optimales et presentent certaines limites, ainsi l'efficacite du DXA est questionnee dans le milieu orthopedique tandis que le QCT necessite des niveaux de radiations problematiques pour en faire un outil de depistage. Les ondes guidees ultrasonores sont utilisees depuis de nombreuses annees pour detecter les fissures, la geometrie et les proprietes mecaniques de cylindres, tuyaux et autres structures dans des milieux industriels. De plus, leur utilisation est plus abordable que celle du DXA et n'engendrent pas de radiation ce qui les rendent prometteuses pour detecter les proprietes mecaniques des os. Depuis moins de dix ans, de nombreux laboratoires de recherche tentent de transposer ces techniques au monde medical, en propageant les ondes guidees ultrasonores dans les os. Le travail presente ici a pour but de demontrer le potentiel des ondes guidees ultrasonores pour determiner l'evolution des proprietes mecaniques de l'os cortical. Il commence par une introduction generale sur les ondes guidees ultrasonores et une revue de la litterature des differentes techniques relatives a l'utilisation des ondes guidees ultrasonores sur les os. L'article redige lors de ma maitrise est ensuite presente. L'objectif de cet article est d'exciter et de detecter certains modes des ondes guides presentant une sensibilite a la deterioration des proprietes mecaniques de l'os cortical. Ce travail est realise en modelisant par elements finis la propagation de ces ondes dans deux modeles osseux

  20. RepB proteins of the multipartite Rhizobium leguminosarum bv. trifolii genome discriminate between centromere-like parS sequences for plasmid segregational stability.

    Science.gov (United States)

    Koper, Piotr; Żebracki, Kamil; Marczak, Małgorzata; Skorupska, Anna; Mazur, Andrzej

    2016-11-01

    The plasmids of the Rhizobiaceae family members and other Alphaproteobacteria are usually large, low copy-number and contain all elements necessary for active segregation and replication located in one operon comprising repABC genes. The genome of Rhizobium leguminosarum bv. trifolii TA1 (RtTA1) consists of a chromosome and four plasmids (pRleTA1a-d) with repABC operons. In this work, centromere-binding RepB proteins of four RtTA1 plasmids were studied. Stability assays of the truncated derivatives of repABC cassettes demonstrated that RepA, RepB proteins and parS-like elements constituted plasmid partitioning systems, while RepC were sufficient for their replication. Individual RepB proteins bound specifically to centromere-like parS elements of the parental plasmids, which was crucial step toward the proper segregation of plasmids into daughter cells. RtTA1 RepB proteins formed dimers and oligomers in the solution. The C-terminal part of RepB was responsible for dimerization, while the domain engaged in parS binding was located in the middle of the protein. It was concluded that the specific interaction between individual RepB proteins and their target sequences together with the substantial diversity of the Rep proteins and parS originating from different plasmids strongly contributed to the coexistence of several plasmids equipped with similar repABC cassettes in the multipartite bacterial genome. © 2016 John Wiley & Sons Ltd.

  1. Profiling gene expression induced by protease-activated receptor 2 (PAR2 activation in human kidney cells.

    Directory of Open Access Journals (Sweden)

    Jacky Y Suen

    Full Text Available Protease-Activated Receptor-2 (PAR2 has been implicated through genetic knockout mice with cytokine regulation and arthritis development. Many studies have associated PAR2 with inflammatory conditions (arthritis, airways inflammation, IBD and key events in tumor progression (angiogenesis, metastasis, but they have relied heavily on the use of single agonists to identify physiological roles for PAR2. However such probes are now known not to be highly selective for PAR2, and thus precisely what PAR2 does and what mechanisms of downstream regulation are truly affected remain obscure. Effects of PAR2 activation on gene expression in Human Embryonic Kidney cells (HEK293, a commonly studied cell line in PAR2 research, were investigated here by comparing 19,000 human genes for intersecting up- or down-regulation by both trypsin (an endogenous protease that activates PAR2 and a PAR2 activating hexapeptide (2f-LIGRLO-NH(2. Among 2,500 human genes regulated similarly by both agonists, there were clear associations between PAR2 activation and cellular metabolism (1,000 genes, the cell cycle, the MAPK pathway, HDAC and sirtuin enzymes, inflammatory cytokines, and anti-complement function. PAR-2 activation up-regulated four genes more than 5 fold (DUSP6, WWOX, AREG, SERPINB2 and down-regulated another six genes more than 3 fold (TXNIP, RARG, ITGB4, CTSD, MSC and TM4SF15. Both PAR2 and PAR1 activation resulted in up-regulated expression of several genes (CD44, FOSL1, TNFRSF12A, RAB3A, COPEB, CORO1C, THBS1, SDC4 known to be important in cancer. This is the first widespread profiling of specific activation of PAR2 and provides a valuable platform for better understanding key mechanistic roles of PAR2 in human physiology. Results clearly support the development of both antagonists and agonists of human PAR2 as potential disease modifying therapeutic agents.

  2. Le Web pour enseigner par projets et favoriser la collaboration

    Directory of Open Access Journals (Sweden)

    Sylvie Ratté

    2004-01-01

    Full Text Available L’utilisation du Web présentée dans cet article vient appuyer une démarche pédagogique fondée sur le partage des savoirs. Dans le contexte d’une simulation industrielle, les étudiants d’un cours de programmation avancée doivent apprendre à partager leur expertise, à communiquer clairement leurs questions, à décrire des problèmes et à faire face à des retournements inattendus. Le Web devient un espace de collaboration pour diffuser les requêtes d’un client fictif, les directives, les travaux préliminaires et les ententes. Les résultats obtenus tendent à suggérer que cette approche globale force l’étudiant à mieux planifier, l’invite à mieux formuler et décrire les problèmes, augmente le niveau de collaboration et facilite la production d’analogies. Un système facilitant la logistique entourant la revue par les pairs (récupération des travaux, construction d’une grille d’évaluation et diffusion des résultats est également présenté.

  3. Monitoreo de parásitos en efluentes domiciliarios

    Directory of Open Access Journals (Sweden)

    Liliana Semenas

    1999-08-01

    Full Text Available OBJETIVO: Evaluar la presencia de parásitos en efluentes semisólidos y líquidos en distintas localidades de la Patagonia argentina considerando que ésta es una de las restricciones para su uso. MÉTODOS: Las muestras tomadas en 4 Plantas de Tratamiento de Efluentes Domiciliarios se analizaron siguiendo las normativas de Environmental Protection Agency, Organización Mundial de la Salud, Standard Methods for Examination of Water and Wastewater y de algunas clasificaciones. RESULTADOS: Solamente 2 de las 6 muestras de semisólidos analizadas tenían huevos de Ascaris lumbricoides no viables. De las 10 muestras líquidas, solamente 2 no tenían huevos mientras las restantes tenían patógenos de las categorías I (Giardia sp., Hymenolepis diminuta y Enterobius vermicularis y III (Ascaris lumbricoides, Ancylostoma duodenale y Trichuris trichiura. CONCLUSIONES: Todas las muestras de semisólidos analizadas fueron aptas para su uso como fertilizantes porque no se registró en ninguna de ellas la presencia de huevos viables de Ascaris lumbricoides y solamente 6 de las muestras líquidas fueron aptas para riego por carecer de huevos o por ser su concentración igual o inferior a 1 huevo por litro.

  4. Characterization of ParTI Phoswiches Using Charged Pion Beams

    Science.gov (United States)

    Churchman, Emily; Zarrella, Andrew; Youngs, Michael; Yennello, Sherry

    2017-09-01

    The Partial Truncated Icosahedron (ParTI) detector array consists of 15 phoswiches. Each phoswich is made of two scintillating components - a thallium-doped cesium iodide (CsI(Tl)) crystal and an EJ-212 scintillating plastic - coupled to a photomultiplier tube. Both materials have different scintillation times and are sensitive to both charged and neutral particles. The type of particle and amount of energy deposited determine the shape of the scintillation pulse as a function of time. By integrating the fast and slow signals of the scintillation pulses, a ``Fast vs. Slow Integration'' plot can be created that produces particle identification lines based on the energy deposited in the scintillating materials. Four of these phoswiches were taken to the Paul Scherrer Institute (PSI) in Switzerland where π + , π-, and proton beams were scattered onto the phoswiches to demonstrate their particle identification (PID) capabilities. Using digitizers to record the detector response waveforms, pions can also be identified by the characteristic decay pulse of the muon daughters.

  5. Monitoreo de parásitos en efluentes domiciliarios

    Directory of Open Access Journals (Sweden)

    Semenas Liliana

    1999-01-01

    Full Text Available OBJETIVO: Evaluar la presencia de parásitos en efluentes semisólidos y líquidos en distintas localidades de la Patagonia argentina considerando que ésta es una de las restricciones para su uso. MÉTODOS: Las muestras tomadas en 4 Plantas de Tratamiento de Efluentes Domiciliarios se analizaron siguiendo las normativas de Environmental Protection Agency, Organización Mundial de la Salud, Standard Methods for Examination of Water and Wastewater y de algunas clasificaciones. RESULTADOS: Solamente 2 de las 6 muestras de semisólidos analizadas tenían huevos de Ascaris lumbricoides no viables. De las 10 muestras líquidas, solamente 2 no tenían huevos mientras las restantes tenían patógenos de las categorías I (Giardia sp., Hymenolepis diminuta y Enterobius vermicularis y III (Ascaris lumbricoides, Ancylostoma duodenale y Trichuris trichiura. CONCLUSIONES: Todas las muestras de semisólidos analizadas fueron aptas para su uso como fertilizantes porque no se registró en ninguna de ellas la presencia de huevos viables de Ascaris lumbricoides y solamente 6 de las muestras líquidas fueron aptas para riego por carecer de huevos o por ser su concentración igual o inferior a 1 huevo por litro.

  6. ANALYSIS OF PARS PLANA VITRECTOMY INCISIONS USING LIVE BACTERIA.

    Science.gov (United States)

    Cohen, Michael N; Houston, Samuel K; Roberts, Amity L; Eagle, Ralph C; Gupta, Omesh P

    2017-06-01

    To analyze small gauge pars plana vitrectomy sclerotomies using live bacteria transformed with green fluorescent protein (GFP). Twenty-eight human cadaver eyes were specially harvested for this study. Small gauge vitrectomy was performed on each eye and the wounds were closed with various techniques (sutured, sutureless, and cauterization). Live Staphylococcus epidermidis that has been transformed with a green fluorescent protein was applied to the overlying conjunctival surface. Analysis of all vitreous samples was analyzed with confocal laser microscopy to identify the presence of bacteria. All wounds were analyzed histopathologically. A high concentration of bacteria was noted in 2 of 3 eyes in the sutureless, 23-G perpendicular incision group postinoculation. There were no bacteria detected in any postvitrectomy sample that were closed with cautery or a beveled incision. No bacteria were found in postvitrectomy samples of sutureless 27-G perpendicular incisions and sutureless 27-G beveled incisions. Finally, there were no bacteria detected in both eyes with 23-G perpendicular incisions that had a partial air-fill. Live bacteria can be effectively used to analyze wound integrity. Closing sclerotomy sites with cautery proved effective in a model using fresh, human cadaver eyes. 27-G perpendicular incisions may be just as competent as 27-G beveled incisions.

  7. Characterization of a dodecapeptide containing a dominant epitope of Par j 1 and Par o 1, the major allergens of P. judaica and P. officinalis pollen.

    Science.gov (United States)

    Menna, T; Cassese, G; Di Modugno, F; Chersi, A; Buono, C; Ruffilli, A

    1999-10-01

    The pollen of Parietaria, a weed of the Urticaceae family, is a major cause of respiratory allergy in Europe, where the most common species are P. judaica and P. officinalis. Previously, we reported that a beta-galactosidase fusion protein (6a-BG) expressing a 26-bp cDNA fragment (6a cDNA) contained a dominant IgE-binding epitope (6a epitope) of the major allergens Par o 1 and Par j 1. The present study aimed to define the amino-acid sequence containing the 6a epitope. We analyzed the reactivity of anti-Par o 1 antibodies affinity purified from allergic patient sera with: 1) a panel of synthetic peptides deduced from the 6a nucleotide sequence using different reading frames 2) glutathione S-transferase (GST) fusion proteins containing selected peptides. The peptide NSARARADSCRI (p102) specifically bound anti-Par o 1 antibodies affinity purified from allergic patient sera or from rabbit anti-Par o 1 antiserum (ELISA). The related peptide NSARAGTSSCRI (p101) reacted to human but not to rabbit, anti-Par o 1 antibodies. GST fusion proteins containing p101 (GST 3.5) or p102 (GST 3.2) extensively inhibited the binding between Par o 1 and IgE or IgG antibodies from an allergic patient serum pool according to a dose-response curve. Percent inhibition of IgE antibodies binding obtained by absorbing a solution (50 microl) of affinity-purified antibodies with 5 microg of GST 3.2 or with 1.2 mg of GST 3.5 was 69% and 66%, respectively. In conclusion, the results of the present study indicate that the amino-acid sequences NSARARADSCRI (p102) and NSARAGTSSCRI (p101) contain the dominant epitope of Par o 1 and Par j 1 for human IgE and IgG antibodies indicated as 6a epitope. Moreover, the study shows that the epitope is conserved in recombinant molecules containing these peptides, irrespective of the fused polypeptide (beta-galactosidase or GST). The knowledge of the amino-acid sequence of this dominant epitope is important in therapeutic approaches to the development of allergen

  8. Effects of solar PAR and UV radiation on tropical biofouling communities

    KAUST Repository

    Dobretsov, SV

    2010-03-08

    We investigated the effect of solar ultraviolet radiation (UVR) and photosynthetically active radiation (PAR) on the development of tropical micro- and macrofouling communities for 30 d. The experimental design involved 3 treatments: full spectrum (PAR+UVR), PAR only, and minimal light (reduced PAR and UVR). Terminal restriction fragment length polymorphism analysis demonstrated that different light conditions resulted in the formation of highly different microbial communities. The lowest densities of bacteria were found under the full spectrum treatment, while the lowest densities of diatoms were found in the minimal light treatment. Macrofouling communities consisted of 13 species and differed among light treatments. In the presence of UVR, communities had low species diversity, evenness, and richness, while in minimal light and PAR treatments, communities had high species diversity, evenness, and richness. Similarity percentage (SIMPER) analysis revealed that the tubeworm Hydroides elegans, the alga Ulva (Enteromorpha) sp., and the bivalve Perna viridis were the species responsible for most of the dissimilarities in macrofouling communities among treatments. While densities of H. elegans were similar in the PAR and minimal light treatments, this polychaete had higher growth rates under minimal light conditions. We conclude that UVR and PAR directly control the development of shallow micro- and macrofouling communities by inhibiting the recruitment and growth of sensitive species and promoting the growth of resistant species, but also that these forms of solar radiation influence the surface cues available to competent larvae by altering the development of the microbial community.

  9. Pars plana Ahmed valve and vitrectomy in patients with glaucoma associated with posterior segment disease.

    Science.gov (United States)

    Wallsh, Josh O; Gallemore, Ron P; Taban, Mehran; Hu, Charles; Sharareh, Behnam

    2013-01-01

    To assess the safety and efficacy of a modified technique for pars plana placement of the Ahmed valve in combination with pars plana vitrectomy in the treatment of glaucoma associated with posterior segment disease. Thirty-nine eyes with glaucoma associated with posterior segment disease underwent pars plana vitrectomy combined with Ahmed valve placement. All valves were placed in the pars plana using a modified technique, without the pars plana clip, and using a scleral patch graft. The 24 eyes diagnosed with neovascular glaucoma had an improvement in intraocular pressure from 37.6 mmHg to 13.8 mmHg and best-corrected visual acuity from 2.13 logarithm of minimum angle of resolution to 1.40 logarithm of minimum angle of resolution. Fifteen eyes diagnosed with steroid-induced glaucoma had an improvement in intraocular pressure from 27.9 mmHg to 14.1 mmHg and best-corrected visual acuity from 1.38 logarithm of minimum angle of resolution to 1.13 logarithm of minimum angle of resolution. Complications included four cases of cystic bleb formation and one case of choroidal detachment and explantation for hypotony. Ahmed valve placement through the pars plana during vitrectomy is an effective option for managing complex cases of glaucoma without the use of the pars plana clip.

  10. Lumbar lordosis and pars interarticularis fractures: a case-control study.

    Science.gov (United States)

    Bugg, William G; Lewis, Mark; Juette, Arne; Cahir, John G; Toms, Andoni P

    2012-07-01

    The aim of this study is to examine the relationship between lumbar lordosis and pars interarticularis fractures. In this retrospective case-control study we compare the angle of lumbar lordosis and the angle of the S1 vertebral endplate (as a measure of pelvic tilt) in patients with bilateral L5 pars interarticularis fractures with age- and sex-matched control cases with normal MRI examinations of the lumbar spine. Twenty-nine cases of bilateral L5 pars interarticularis fractures with matched control-cases were identified on MRI (16 male, 13 female, age 9-63 years). The angle of lordosis was measured between the inferior L4 and superior S1 vertebral endplates on a standing lateral lumbar spine radiograph for both groups. The mean angle of lordosis about the L5 vertebra was 36.9° (SD = 6.5°) in the pars interarticularis fracture group, and 30.1° (SD = 6.4°) in the control group. The difference between the two groups was significant (mean difference 6.8°, Student's t test: P pars defects. The difference in the means of 14.2° was statistically significantly different (P pars fractures at L5. Steep angulation of the first sacral vertebral segment maybe the predisposing biomechanical factor that leads to pincer-like impingement of the pars interarticularis and then spondylolysis.

  11. The pars intermedia: an anatomic basis for a coordinated vascular response to female genital arousal.

    Science.gov (United States)

    Shih, Cheryl; Cold, Christopher J; Yang, Claire C

    2013-06-01

    The pars intermedia is an area of the vulva that has been inconsistently described in the literature. We conducted anatomic studies to better describe the tissues and vascular structures of the pars intermedia and proposed a functional rationale of the pars intermedia in the female sexual response. Nine cadaveric vulvectomy specimens were used. Each was serially sectioned and stained with hematoxylin and eosin and Masson's trichrome. Histologic ultrastructural description of the pars intermedia. The pars intermedia contains veins traveling longitudinally in the angle of the clitoris, supported by collagen-rich stromal tissues. These veins drain the different vascular compartments of the vulva, including the clitoris, the bulbs, and labia minora; also, the interconnecting veins link the different vascular compartments. The pars intermedia is not composed of erectile tissue, distinguishing it from the erectile tissues of the corpora cavernosa of the clitoris as well as the corpus spongiosum of the clitoral (vestibular) bulbs. The venous communications of the pars intermedia, linking the erectile tissues with the other vascular compartments of the vulva, appear to provide the anatomic basis for a coordinated vascular response during female sexual arousal. © 2012 International Society for Sexual Medicine.

  12. A mechanism for ParB-dependent waves of ParA, a protein related to DNA segregation during cell division in prokaryotes.

    Science.gov (United States)

    Hunding, Axel; Ebersbach, Gitte; Gerdes, Kenn

    2003-05-23

    Prokaryotic plasmids encode partitioning (par) loci involved in segregation of DNA to daughter cells at cell division. A functional fusion protein consisting of Walker-type ParA ATPase and green fluorescent protein (Gfp) oscillates back and forth within nucleoid regions with a wave period of about 20 minutes. A model is discussed which is based on cooperative non-specific binding of ParA to the nucleoid, and local ParB initiated generation of ParA oligomer degradation products, which act autocatalytically on the degradation reaction. The model yields self-initiated spontaneous pattern formation, based on Turing's mechanism, and these patterns are destroyed by the degradation products, only to initiate a new pattern at the opposite nucleoid region. A recurrent wave thus emerges. This may be a particular example of a more general class of pattern forming mechanisms, based on protein oligomerization upon a template (membranes, DNA a.o.) with resulting enhanced NTPase function in the oligomer state, which may bring the oligomer into an unstable internal state. An effector initializes destabilization of the oligomer to yield degradation products, which act as seeds for further degradation in an autocatalytic process. We discuss this mechanism in relation to recent models for MinDE oscillations in E.coli and to microtubule degradation in mitosis. The study points to an ancestral role for the presented pattern types in generating bipolarity in prokaryotes and eukaryotes.

  13. Proteinase-activated receptors (PARs) – focus on receptor-receptor-interactions and their physiological and pathophysiological impact

    Science.gov (United States)

    2013-01-01

    Proteinase-activated receptors (PARs) are a subfamily of G protein-coupled receptors (GPCRs) with four members, PAR1, PAR2, PAR3 and PAR4, playing critical functions in hemostasis, thrombosis, embryonic development, wound healing, inflammation and cancer progression. PARs are characterized by a unique activation mechanism involving receptor cleavage by different proteinases at specific sites within the extracellular amino-terminus and the exposure of amino-terminal “tethered ligand“ domains that bind to and activate the cleaved receptors. After activation, the PAR family members are able to stimulate complex intracellular signalling networks via classical G protein-mediated pathways and beta-arrestin signalling. In addition, different receptor crosstalk mechanisms critically contribute to a high diversity of PAR signal transduction and receptor-trafficking processes that result in multiple physiological effects. In this review, we summarize current information about PAR-initiated physical and functional receptor interactions and their physiological and pathological roles. We focus especially on PAR homo- and heterodimerization, transactivation of receptor tyrosine kinases (RTKs) and receptor serine/threonine kinases (RSTKs), communication with other GPCRs, toll-like receptors and NOD-like receptors, ion channel receptors, and on PAR association with cargo receptors. In addition, we discuss the suitability of these receptor interaction mechanisms as targets for modulating PAR signalling in disease. PMID:24215724

  14. Identification of uPAR-positive chemoresistant cells in small cell lung cancer.

    Directory of Open Access Journals (Sweden)

    Margarita Gutova

    Full Text Available BACKGROUND: The urokinase plasminogen activator (uPA and its receptor (uPAR/CD87 are major regulators of extracellular matrix degradation and are involved in cell migration and invasion under physiological and pathological conditions. The uPA/uPAR system has been of great interest in cancer research because it is involved in the development of most invasive cancer phenotypes and is a strong predictor of poor patient survival. However, little is known about the role of uPA/uPAR in small cell lung cancer (SCLC, the most aggressive type of lung cancer. We therefore determined whether uPA and uPAR are involved in generation of drug resistant SCLC cell phenotype. METHODS AND FINDINGS: We screened six human SCLC cell lines for surface markers for putative stem and cancer cells. We used fluorescence-activated cell sorting (FACS, fluorescence microscopy and clonogenic assays to demonstrate uPAR expression in a subpopulation of cells derived from primary and metastatic SCLC cell lines. Cytotoxic assays were used to determine the sensitivity of uPAR-positive and uPAR-negative cells to chemotherapeutic agents. The uPAR-positive cells in all SCLC lines demonstrated multi-drug resistance, high clonogenic activity and co-expression of CD44 and MDR1, putative cancer stem cell markers. CONCLUSIONS: These data suggest that uPAR-positive cells may define a functionally important population of cancer cells in SCLC, which are resistant to traditional chemotherapies, and could serve as critical targets for more effective therapeutic interventions in SCLC.

  15. MMP-1/PAR-1 signal transduction axis and its prognostic impact in esophageal squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Hong-hua Peng

    2012-01-01

    Full Text Available The matrix metalloprotease-1 (MMP-1/protease-activated receptor-1 (PAR-1 signal transduction axis plays an important role in tumorigenesis. To explore the expression and prognostic value of MMP-1 and PAR-1 in esophageal squamous cell carcinoma (ESCC, we evaluated the expression of two proteins in resected specimens from 85 patients with ESCC by immunohistochemistry. Sixty-two (72.9% and 58 (68.2% tumors were MMP-1- and PAR-1-positive, respectively, while no significant staining was observed in normal esophageal squamous epithelium. MMP-1 and PAR-1 overexpression was significantly associated with tumor node metastasis (TNM stage and regional lymph node involvement. Patients with MMP-1- and PAR-1-positive tumors, respectively, had poorer disease-free survival (DFS than those with negative ESCC (P = 0.002 and 0.003, respectively. Univariate analysis showed a significant relationship between TNM stage [hazard ratio (HR = 2.836, 95% confidence interval (CI = 1.866-4.308], regional lymph node involvement (HR = 2.955, 95%CI = 1.713-5.068, MMP-1 expression (HR = 2.669, 95%CI = 1.229-6.127, and PAR-1 expression (HR = 1.762, 95%CI = 1.156-2.883 and DFS. Multivariate analysis including the above four parameters identified TNM stage (HR = 2.035, 95%CI = 1.167-3.681, MMP-1 expression (HR = 2.109, 95%CI = 1.293-3.279, and PAR-1 expression (HR = 1.967, 95%CI = 1.256-2.881 as independent and significant prognostic factors for DFS. Our data suggest for the first time that MMP-1 and PAR-1 were both overexpressed in ESCC and are novel predictors of poor patient prognosis after curative resection. The MMP-1/PAR-1 signal transduction axis might be a new therapeutic target for future therapies tailored against ESCC.

  16. The urokinase receptor (uPAR facilitates clearance of Borrelia burgdorferi.

    Directory of Open Access Journals (Sweden)

    Joppe W R Hovius

    2009-05-01

    Full Text Available The causative agent of Lyme borreliosis, the spirochete Borrelia burgdorferi, has been shown to induce expression of the urokinase receptor (uPAR; however, the role of uPAR in the immune response against Borrelia has never been investigated. uPAR not only acts as a proteinase receptor, but can also, dependently or independently of ligation to uPA, directly affect leukocyte function. We here demonstrate that uPAR is upregulated on murine and human leukocytes upon exposure to B. burgdorferi both in vitro as well as in vivo. Notably, B. burgdorferi-inoculated C57BL/6 uPAR knock-out mice harbored significantly higher Borrelia numbers compared to WT controls. This was associated with impaired phagocytotic capacity of B. burgdorferi by uPAR knock-out leukocytes in vitro. B. burgdorferi numbers in vivo, and phagocytotic capacity in vitro, were unaltered in uPA, tPA (low fibrinolytic activity and PAI-1 (high fibrinolytic activity knock-out mice compared to WT controls. Strikingly, in uPAR knock-out mice partially backcrossed to a B. burgdorferi susceptible C3H/HeN background, higher B. burgdorferi numbers were associated with more severe carditis and increased local TLR2 and IL-1beta mRNA expression. In conclusion, in B. burgdorferi infection, uPAR is required for phagocytosis and adequate eradication of the spirochete from the heart by a mechanism that is independent of binding of uPAR to uPA or its role in the fibrinolytic system.

  17. The Urokinase Receptor (uPAR) Facilitates Clearance of Borrelia burgdorferi

    Science.gov (United States)

    Hovius, Joppe W. R.; Bijlsma, Maarten F.; van der Windt, Gerritje J. W.; Wiersinga, W. Joost; Boukens, Bastiaan J. D.; Coumou, Jeroen; Oei, Anneke; de Beer, Regina; de Vos, Alex F.; Veer, Cornelis van 't; van Dam, Alje P.; Wang, Penghua; Fikrig, Erol; Levi, Marcel M.; Roelofs, Joris J. T. H.; van der Poll, Tom

    2009-01-01

    The causative agent of Lyme borreliosis, the spirochete Borrelia burgdorferi, has been shown to induce expression of the urokinase receptor (uPAR); however, the role of uPAR in the immune response against Borrelia has never been investigated. uPAR not only acts as a proteinase receptor, but can also, dependently or independently of ligation to uPA, directly affect leukocyte function. We here demonstrate that uPAR is upregulated on murine and human leukocytes upon exposure to B. burgdorferi both in vitro as well as in vivo. Notably, B. burgdorferi-inoculated C57BL/6 uPAR knock-out mice harbored significantly higher Borrelia numbers compared to WT controls. This was associated with impaired phagocytotic capacity of B. burgdorferi by uPAR knock-out leukocytes in vitro. B. burgdorferi numbers in vivo, and phagocytotic capacity in vitro, were unaltered in uPA, tPA (low fibrinolytic activity) and PAI-1 (high fibrinolytic activity) knock-out mice compared to WT controls. Strikingly, in uPAR knock-out mice partially backcrossed to a B. burgdorferi susceptible C3H/HeN background, higher B. burgdorferi numbers were associated with more severe carditis and increased local TLR2 and IL-1β mRNA expression. In conclusion, in B. burgdorferi infection, uPAR is required for phagocytosis and adequate eradication of the spirochete from the heart by a mechanism that is independent of binding of uPAR to uPA or its role in the fibrinolytic system. PMID:19461880

  18. uPAR Expression Pattern in Patients with Urothelial Carcinoma of the Bladder

    DEFF Research Database (Denmark)

    Dohn, Line Hammer; Pappot, Helle; Iversen, Benedikte Richter

    2015-01-01

    The objective of the present study was to confirm the expression and localisation pattern of the urokinase-type plasminogen activator receptor (uPAR) focusing on its possible clinical relevance in patients with urothelial neoplasia of the bladder. uPAR is a central molecule in tissue remodelling...... during cancer invasion and metastasis and is an established prognostic marker in various cancer diseases other than bladder cancer. Formalin-fixed and paraffin-embedded tumour-tissue blocks from 186 patients treated with radical cystectomy were analysed. uPAR expression was scored as either negative...

  19. Los parásitos de los animales domésticos.

    OpenAIRE

    Cruz B. Luis

    2012-01-01

    Desde el año 1937, el Departamento Nacional de Agricultura, desean prestar un servicio más efectivo para el agricultor costarricense, viene publicando en su Revista Agrícola, artículos referentes a los parásitos que infestan nuestro ganados e incluyendo siempre las medidas más prácticas para su control. Durante muchos años, los parásitos de los animales han constituido un verdadero flagelo para la industria costarricense, entorpeciendo su progreso; pero desde que el Servicio de Par...

  20. Profils des enfants infectes par le vih en debut du traitement ...

    African Journals Online (AJOL)

    But : Décrire les profils des enfants infectés par le VIH au début du traitement antirétroviral. Matériels et méthode: Les dossiers des enfants infectés par le VIH dans la région maritime ont été analysés de mai 2008 à février 2010 par le comité thérapeutique. Résultats: Parmi les 96 dossiers analysés, 66,67% venaient du ...

  1. Les dispensées par fantaisie en éducation physique et sportive ...

    African Journals Online (AJOL)

    Cette étude vise à comprendre les raisons avancées par les élèves dispensés par fantaisie des cours d'EPS, pour justifier leur attitude et principalement leur forfait. Une enquête par questionnaire écrit a été menée dans 13 établissements publics de la ville de Porto-Novo au Bénin. Au total, 203 élèves parmi les 771 élèves ...

  2. Data on the regulation of moesin and merlin by the urokinase receptor (uPAR): Model explaining distal activation of integrins by uPAR.

    Science.gov (United States)

    Degryse, Bernard; Britto, Mishan; Shan, Chun Xu; Wallace, Robert G; Rochfort, Keith D; Cummins, Philip M; Meade, Gerardene; Murphy, Ronan P

    2017-12-01

    The data presented herein are connected to our research article (doi: 10.1016/j.biocel.2017.04.012) [1], in which we investigated the functional connections between the urokinase receptor (uPAR), and the ezrin/radixin/moesin (ERM) proteins, moesin and merlin [1]. Firstly, a model of action is proposed that enlightens how uPAR regulates distal integrins. In addition, data show the effects of expressing wild-type moesin or permanently active T558D mutant of moesin on angiogenesis and morphology of human aortic endothelial cells (HAEC). Additional data compare the effects of urokinase (uPA, the main ligand of uPAR) on the same cells. Lastly, we provide technical data demonstrating the effects of specific siRNA for moesin and merlin on moesin and merlin expression, respectively.

  3. Data on the regulation of moesin and merlin by the urokinase receptor (uPAR: Model explaining distal activation of integrins by uPAR

    Directory of Open Access Journals (Sweden)

    Bernard Degryse

    2017-12-01

    Full Text Available The data presented herein are connected to our research article (doi: 10.1016/j.biocel.2017.04.012 [1], in which we investigated the functional connections between the urokinase receptor (uPAR, and the ezrin/radixin/moesin (ERM proteins, moesin and merlin [1]. Firstly, a model of action is proposed that enlightens how uPAR regulates distal integrins. In addition, data show the effects of expressing wild-type moesin or permanently active T558D mutant of moesin on angiogenesis and morphology of human aortic endothelial cells (HAEC. Additional data compare the effects of urokinase (uPA, the main ligand of uPAR on the same cells. Lastly, we provide technical data demonstrating the effects of specific siRNA for moesin and merlin on moesin and merlin expression, respectively. Keywords: Urokinase receptor, Moesin, Merlin, Angiogenesis, siRNA

  4. Caractérisation des changements de phase développés dans un alliage AMF du type Cu Al Ni, par dilatométrie de trempe et microcalorimétrie différentielle

    Science.gov (United States)

    Bouabdallah, M.; Cizeron, G.

    1998-02-01

    The structural behaviour of a SM alloy Cu{-}Al{-}Ni type, manufactured by casting then hot extruded and finally quenched, has been studied by dilatometry and DSC. The resulting martensitic structure (β'_1) is characterized by a noticeable texture; this explains that a memory effect (shown by a contraction) is observed on the dilatometric curve during β'_1rightarrow β_1 transformation when heating (V_h=10 ^circC s^{-1}). At higher temperature, the β_1 phase is disordered in a β (A2) structure. When reverting, the β rightarrowβ_1 ordering occurs in two steps whatever the cooling rate (V_c). On the contrary, at lower temperature, Ms and Mf points linked to the martensite β_1rightarrowβ'_1 transformation vary with V_c and decrease when the cooling rate is high enough. Moreover DSC tests performed at V_h = V_c = 2 ^circC min^{-1} on the as quenched alloy gave evidence of aging processes occuring in the β_1 phase between 200-350 ^circ C; very small precipitates are then formed which dissolve partially when heating from 380 to 440 ^circ C. At still higher temperatures (i.e. between 500 and 560 ^circ C) the β_1 phase decomposes in α and γ_2 (equilibrium phases) while this binary system gives the β phase finally. When cooling at this low rate, the alloy previously heated up to 620 ^circ C, transforms back to (α + γ_2) according to an eutectoïd mode as predicted by the equilibrium diagram. Les évolutions structurales développées dans un alliage à mémoire de forme du type Cu Ni Al (préalablement élaboré par fonderie, puis filé à chaud et finalement trempé à l'eau) ont été caractérisées par dilatométrie et analyse différentielle. La structure résultant de cette suite de traitements est du type martensite β'_1 ; elle présente une texture marquée, ce qui justifie qu'un effet mémoire se manifeste, sur la courbe dilatométrique au chauffage (10 ^circ C/s) lequel se traduit par une contraction lors de la transition β'_1rightarrowβ_1.

  5. La catalanitat a les marques de begudes catalanes

    Directory of Open Access Journals (Sweden)

    Berta Cantó Gargallo

    2014-07-01

    Full Text Available Aquesta tesina posa de manifest la gran importància que té la publicitat per a configurar els imaginaris col·lectius, així com identitaris, i la manera com aquests serveixen per identificar-nos com a grup. Per mitjà no només de l’anàlisi de les campanyes publicitàries sinó també de la llengua de l’etiquetatge, el domini web, els patrocinis, les col·laboracions i les iniciatives que cada marca duu a terme, el lector i consumidor es fa una idea de quins són els elements icònics i verbals que ens defineixen com a poble i de quina manera cadascuna d’aquestes els empra per transmetre la seva versió de la catalanitat.

  6. Les coopératives vinicoles entre marques et terroirs

    OpenAIRE

    Schirmer, Raphaël

    2007-01-01

    Nées lors des crises de la fin du XIXe et du début du XX siècle, les coopératives vinicoles apparaissent désormais comme un acteur majeur de la filière dans un contexte de mondialisation des marchés. Même si leur situation paraît très contrastée sur le territoire français, elles ont fait preuve d'une grande capacité de modernisation des étapes qui encadrent ou suivent la vinification. Elles concentrent désormais leurs efforts sur les processus situés en amont, dans les vignes. Loin de produir...

  7. Tee Bagdadini: 21. sajandi ristisõdijad / Tiago Marques

    Index Scriptorium Estoniae

    Marques, Tiago

    2003-01-01

    Kas USA on tegelikult pühendunud "režiimivahetuse" poliitikale Iraagis või tahab kontrollida terve piirkonna majanduspotentsiaali, küsib autor. Kasvavast lõhest USA valitsuses. Regionaalsetest konfliktidest islamimaailmas. Kaart: Iraagi etniline jaotumus

  8. Eesti edu ei sõltu ainult eurofondidest / Tiago Marques

    Index Scriptorium Estoniae

    Marques, Tiago

    2003-01-01

    Portugali ajakirjaniku hinnangul on Euroopa Liidu teemaline debatt keskendunud teisejärgulisele - eurofondidele ja rahvusliku identiteedi kaotamisele. Tegelikult on oluline EL-iga liitumisel acquis communautaire'i rakendumine Eestis

  9. Resurrecting Letters of Marque and Reprisal to Address Modern Threats

    Science.gov (United States)

    2013-03-01

    power projection and national expansion, was largely motivated by the need to protect national maritime trade from mercantile competitors and... Mercantilism from the 16th to the 18th centuries regarded trade, piracy, and warfare as equals in the field of international competition. As a result...times comparable with those of sovereigns.102 Each was granted great latitude by their governments to ensure their Empire’s mercantile prosperity

  10. Urine suPAR levels compared with plasma suPAR levels as predictors of post-consultation mortality risk among individuals assumed to be TB-negative: a prospective cohort study

    DEFF Research Database (Denmark)

    Rabna, Paulo; Andersen, Andreas; Wejse, Christian

    2010-01-01

    -suPAR), thereby exploring the possibility of replacing the blood sample with an easy obtainable urine sample. We enrolled 1,007 adults, older than 15 years of age, with a negative TB diagnosis between April 2004 and December 2006. Levels of U-suPAR and P-suPAR were available in 863 individuals. U......-suPAR was measured using a commercial ELISA (suPARnostic®). We found that U-suPAR carried significant prognostic information on mortality for HIV-infected subjects with an area under the ROC curve of 0.75. For HIV-negative individuals, little or no prognostic effect was observed. However, in both HIV positives...... and negatives, the predictive effect of U-suPAR was found to be inferior to that of P-suPAR....

  11. [Fiberless miniature chandelier LED endoilluminator for pars plana vitrectomy].

    Science.gov (United States)

    Kölbl, P S; Lindner, C; Lingenfelder, C; Deuchler, S; Singh, P; Koch, F H J; Heßling, M

    2016-01-01

    Currently available chandelier endoilluminators for pars plana vitrectomy consist of conventional optical fibers coupled to a light source. The light probes of these fibers now provide wide emittance angles but it is still often not possible to illuminate the whole intraocular space via just one incision. Therefore, several light probes or additional handheld endoilluminators have to be used simultaneously or the lights have to be repositioned during surgery. The presented prototype of a fiberless chandelier light-emitting diode (LED) endoilluminator aims at illuminating the whole intraocular space with just one incision while reducing the risk of harming the retina. The light source is a white LED with a conical tip that allows stable fixation within an incision. The physical properties of these LEDs were determined and used for calculating the relevant irradiance to assess the risk of causing harm to the eye. The illumination of the intraocular space was investigated using porcine eyes. The illumination of porcine eyes with the modified LEDs was bright and homogeneous. Measurements and subsequent calculations proved that the expected thermal load and photochemical hazard were very low for human eyes. The hitherto existing experimental results on porcine eyes and the theoretical considerations on human eyes were found to be positive; therefore, it is expected that this new fiberless chandelier LED endoilluminator will prove to be advantageous for human patients. It promises a reduced number of incisions with a simplified handling and need to add handheld light sources only if minimal intensity oblique illumination is helpful together with the chandelier illumination. This contributes to a significant reduction of phototoxicity risks and additionally there is a chance for a cost reduction because expensive xenon or mercury lamps are no longer necessary. These expectations have to be verified by further studies on human eyes.

  12. Mesencephalic origin of the rostral Substantia nigra pars reticulata.

    Science.gov (United States)

    Madrigal, M Pilar; Moreno-Bravo, Juan A; Martínez-López, Jesús E; Martínez, Salvador; Puelles, Eduardo

    2016-04-01

    In embryonic development, the neurons that will constitute a heterogeneous nucleus may have distinct origins. The different components of these populations reach their final location by radial and tangential migrations. The Substantia nigra pars reticulata (SNR) presents a high level of neuronal heterogeneity. It is composed by GABAergic neurons located in the mes-diencephalic basal plate. These inhibitory neurons usually display tangential migrations and it has been already described that the caudal SNR is colonized tangentially from rhombomere 1. Our aim is to unveil the origin of the rostral SNR. We have localized a Nkx6.2 positive ventricular domain located in the alar midbrain. Nkx6.2 derivatives' fate map analysis showed mainly a rostral colonization of this GABAergic neuronal population. We confirmed the mesencephalic origin by the expression of Six3. Both transcription factors are sequentially expressed along the differentiation of these neurons. We demonstrated the origin of the rostral SNR; our data allowed us to postulate that this nucleus is composed by two neuronal populations distributed in opposite gradients with different origins, one from rhombomere 1, caudal to rostral, and the other from the midbrain, rostral to caudal. We can conclude that the SNR has multiple origins and follows complex mechanisms of specification and migration. Our results support vital information for the study of genetic modifications in these extremely complex processes that result in devastating behavioral alterations and predisposition to psychiatric diseases. Understanding the development, molecular identity and functional characteristics of these diverse neuronal populations might lead to better diagnosis and treatment of several forms of neurological and psychiatric disease.

  13. La relecture du romancero par les vihuelistes de la Renaissance

    Directory of Open Access Journals (Sweden)

    Pascal Martel

    1999-01-01

    Full Text Available Les romances espagnols de la Reconquête relataient sur le mode épique des faits passés sur une mélodie récurrente qui servait surtout d’outil mnémotechnique pour réciter de longues narrations. Lorsque Diego Pisador reprend un romance, il n’en prend que quelques vers : du coup, le rapport entre les mots et les notes se fait plus étroit. D’un poème épique, il élabore une amplification lyrique, une véritable composition musicale, opère un véritable changement d’esthétique : le passage de la longue narration sur un thème musical bref et récurrent qui caractérisait le romance médiéval de la Reconquista, à une forme musico-littéraire plus brève mais plus élaborée, propre à introduire la vibration lyrique dans la matière épique. Familier de la Cour, il entend ainsi servir les desseins politiques de Philippe Il à qui il dédie son œuvre : le compositeur vihueliste qu’était Pisador en effet participe à l’effort de l’intégration du Maure poursuivi par la politique de la Maison d’Autriche. Mais les noces n’eurent pas lieu et les Maures furent au contraire expulsés en 1609. L’analyse de cette chanson nous donne l’occasion de découvrir les moyens employés par le musicien pour se livrer à une véritable amplification lyrique d’un simple récit par la musique. La chanson savante n’est pas non plus une composition dans laquelle la mélodie traduirait simplement en musique le texte poétique de façon tout à fait parallèle : la musique n’est pas qu’une répétition, sous une autre forme, de la parole, ou bien l’image sonore d’un texte. Musique et poésie constituent deux moyens autonomes d’expression mais qui, dans une chanson, concourent à un même but : ces deux moyens d’expression tantôt se rejoignant, tantôt s’émancipant l’un de l’autre, de sorte que le propre de la chanson réside justement dans cette tension constante entre ses deux composantes. La mise en musique

  14. A mechanism for ParB-dependent waves of ParA, a protein related to DNA segregation during cell division in prokaryotes

    DEFF Research Database (Denmark)

    Hunding, Axel; Gerdes, Kenn; Charbon, Gitte Ebersbach

    2003-01-01

    Prokaryotic plasmids encode partitioning (par) loci involved in segregation of DNA to daughter cells at cell division. A functional fusion protein consisting of Walker-type ParA ATPase and green fluorescent protein (Gfp) oscillates back and forth within nucleoid regions with a wave period of about...... in an autocatalytic process. We discuss this mechanism in relation to recent models for MinDE oscillations in E.coli and to microtubule degradation in mitosis. The study points to an ancestral role for the presented pattern types in generating bipolarity in prokaryotes and eukaryotes....

  15. ParTIES: a toolbox for Paramecium interspersed DNA elimination studies.

    Science.gov (United States)

    Denby Wilkes, Cyril; Arnaiz, Olivier; Sperling, Linda

    2016-02-15

    Developmental DNA elimination occurs in a wide variety of multicellular organisms, but ciliates are the only single-celled eukaryotes in which this phenomenon has been reported. Despite considerable interest in ciliates as models for DNA elimination, no standard methods for identification and characterization of the eliminated sequences are currently available. We present the Paramecium Toolbox for Interspersed DNA Elimination Studies (ParTIES), designed for Paramecium species, that (i) identifies eliminated sequences, (ii) measures their presence in a sequencing sample and (iii) detects rare elimination polymorphisms. ParTIES is multi-threaded Perl software available at https://github.com/oarnaiz/ParTIES. ParTIES is distributed under the GNU General Public Licence v3. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  16. PAR-Aware Large-Scale Multi-User MIMO-OFDM Downlink

    CERN Document Server

    Studer, Christoph

    2012-01-01

    We investigate an orthogonal frequency-division multiplexing (OFDM)-based downlink transmission scheme for large-scale multi-user (MU) multiple-input multiple-output (MIMO) wireless systems. The use of OFDM causes a high peak-to-average (power) ratio (PAR), which necessitates expensive and power-inefficient radio-frequency (RF) components at the base station. In this paper, we present a novel downlink transmission scheme, which exploits the massive degrees-of-freedom available in large-scale MU-MIMO-OFDM systems to achieve low PAR. Specifically, we propose to jointly perform MU precoding, OFDM modulation, and PAR reduction by solving a convex optimization problem. We develop a corresponding fast iterative truncation algorithm (FITRA) and show numerical results to demonstrate tremendous PAR-reduction capabilities. The significantly reduced linearity requirements eventually enable the use of low-cost RF components for the large-scale MU-MIMO-OFDM downlink.

  17. BOREAS TE-09 PAR & Leaf Nitrogen Data for NSA, R1

    Data.gov (United States)

    National Aeronautics and Space Administration — This data table contains data collected by TE09 on the relationship between PAR and foliage nitrogen in the canopies of NSA-OBS, NSA-UBS, NSA-YJP, NSA-OJP and...

  18. A biomechanic study of the surgical repair technique of pars defect in spondylolysis.

    Science.gov (United States)

    Vathana, P; Prasartritha, T

    1998-11-01

    To find out which wiring technique of direct repair of the pars defect is the strongest in resisting anteroposterior translation displacement, fifteen fresh human cadaveric L4-L5 spines were biomechanically tested by a universal testing machine. Two millimeters wide pars defect was created on both sides of L4 vertebrae. Each of the five specimens was wired using Nicol's technique (A), modified Nicol's technique (B) and modified pedicular screw technique respectively (C). At each test, motion was observed to occur initially at the pars defect. The mean minimum tensile strength (increment of the pars defect) for technique A, B and C was 87.64, 82.04 and 110.08 Kg Force respectively. By statistical analysis, technique C was the strongest in resisting anteroposterior displacement of the spinal column. There was no statistically significant difference between technique A and B.

  19. PARS PLANA VITRECTOMY FOR LATE VITREORETINAL SEQUELAE OF INFECTIOUS ENDOPHTHALMITIS: Surgical Management and Outcomes.

    Science.gov (United States)

    Thomas, Benjamin J; Mehta, Neesurg; Yonekawa, Yoshihiro; Sridhar, Jayanth; Kuriyan, Ajay E; Relhan, Nidhi; Liang, Michelle C; Woodward, Maria A; Witkin, Andre J; Shah, Chirag; Flynn, Harry W; Garg, Sunir J; Wolfe, Jeremy D

    2017-04-01

    To report the visual acuity outcomes after pars plana vitrectomy for delayed vitreoretinal sequelae of infectious endophthalmitis. All eyes were initially treated with intravitreal antibiotics (Abx). Multicenter, retrospective, consecutive case series. Forty-two eyes met the study criteria. The mean follow-up was 48 weeks (SD ± 61.8). Mean interval from Abx to pars plana vitrectomy was 13 weeks (SD ± 14.3, range 2-70). Indications for pars plana vitrectomy included vitreous opacities (VO) (n = 22), epiretinal membrane (n = 9), and retinal detachment (n = 11). LogMAR visual acuity improved from 1.87 (Snellen equivalent: 20/1,482) preoperatively to 1.35 (Snellen equivalent: 20/447) at final evaluation (P pars plana vitrectomy for delayed sequelae such as vitreous opacities or for retinal detachment.

  20. Detection of pars plana rupture by ultrasound biomicroscopy after cannula dislodgement during cataract wound hydration.

    Science.gov (United States)

    Yonekawa, Yoshihiro; Thomas, Benjamin J; Lau-Sickon, Laurie K; Anderson, Bradley J; Ruby, Alan J

    2017-01-01

    Ultrasound biomicroscopy (UBM) is a valuable diagnostic modality for imaging anterior ocular structures. Its utility has been well studied in anterior segment, lenticular, and pars plicata pathologies. However, imaging of the pars plana has been seldom described. We present the case of a 66-year-old woman referred for vitreous hemorrhage after expulsive cannula dislodgement into the posterior segment during wound hydration at the end of cataract surgery. B-scan ultrasonography initially detected a very anterior abnormality, but the resolution was insufficient for accurate diagnosis. Subsequent UBM clearly showed rupture of the pars plana and a mild cyclodialysis cleft. To our knowledge, this is the first report of a pars plana rupture detected by ultrasound, which expands the diagnostic capacities and indications for UBM.

  1. Inmunidad frente a infecciones: bacterias, virus, hongos y parásitos

    OpenAIRE

    Sen Fernández, María Luz de la; Sempere Ortells, José Miguel; Marco de la Calle, Francisco Manuel; Vázquez Araujo, Begoña

    2012-01-01

    Respuesta inmune frente a la infección: Bacterias extracelulares, bacterias intracelulares, hongos, virus, parásitos. Inmunidad innata, inmunidad adaptativa, mecanismos de evasión, consecuencias perjudiciales de la respuesta inmune.

  2. Application of passive auto catalytic recombiner (PAR) for BWR plants in Japan

    Energy Technology Data Exchange (ETDEWEB)

    Kobayashi, K.; Murano, K. [Tokyo Electric Power Co., Inc. (Japan); Yamanari, S. [Hitachi Cable, Ltd., Tokyo (Japan); Yamamoto, Y. [Toshiba Corp., Yokohama (Japan)

    2001-07-01

    The passive auto-catalytic recombiner (PAR), which can recombine flammable gases such as hydrogen and oxygen with each other to avoid an explosion in case of a loss-of-coolant accident (LOCA), installed in the primary containment vessel does not require a power supply or dynamic equipment, while the existing flammability gas control system (FCS) of most BWRs as an outer loop of the primary containment vessel needs them to make flammable gases circulate through blowers and heaters in the system. PAR offers a number of advantages over existing FCS, such as high reliability, low cost due to much smaller amount of materials needed, good maintainability, good operability in case of a LOCA, and smaller space for installation. An experimental study has been carried out for the purpose of solving the problems of applying PAR to Japanese BWR plants instead of existing FCS, in which we grasped the basic characteristics of PAR. (author)

  3. La rebelión del Marqués del Valle: un examen del gobierno virreinal en Nueva España en 1566 = The Rebellion of the Marquis of the Valley: A Test for the Viceregal Government in New Spain at 1566

    Directory of Open Access Journals (Sweden)

    Reiko Tateiwa Igarashi

    2016-12-01

    Full Text Available ResumenEl presente trabajo analiza las diversas fuerzas políticas (visitador, virrey, Audiencia y Consejo de Indias, que actuaron e intervinieron durante los primeros gobiernos virreinales de Nueva España en el siglo XVI, a través de la rebelión del marqués del Valle, Martín Cortés, hijo y heredero del conquistador Hernán Cortés a quien consideraron como líder natural los criollos, descendientes de los conquistadores que reclamaban al rey su derecho de sucesión en las encomiendas, el cual se había visto limitado por las Leyes Nuevas. Lejos de responder a la reclamación de estos criollos, las autoridades real y virreinal revelaron su dificultad para comprender la realidad novohispana y la falta de coordinación política entre la metrópoli y la capital mexicana. Finalmente, se decidieron a introducir cambios en su política, entre ellos, la posibilidad de enviar al príncipe heredero Felipe a las Indias con el fin de establecer el orden. AbstractThis paper analyses the attitude of viceregal political institutions –such as the royal visitador, the Viceroy, the royal Audiencia, the Council of Indies–, during the first governments of New Spain in the 16th century through the rebellion of the Marquis of the Valley, Martín Cortés, conqueror Hernán Cortés’ son, natural leader of the second generation of the conquerors who demanded the King their right to the encomienda, limited by the New Laws. Far from giving a response to the criollos, the real and viceregal authorities showed their difficulties to understand the reality of the Indies and lack of coordination on its policies between the metropolis and its colony. Finally, they started to introduce changes, including the possibility to send prince Felipe to the Indies in order to establish the royal authority.

  4. Paracoccidioides brasiliensis induces cytokine secretion in epithelial cells in a protease-activated receptor-dependent (PAR) manner.

    Science.gov (United States)

    de Oliveira, Priscila; Juliano, Maria Aparecida; Tanaka, Aparecida Sadae; Carmona, Adriana Karaoglanovic; Dos Santos, Saara Maria Batista; de Barros, Bianca Carla Silva Campitelli; Maza, Paloma Korehisa; Puccia, Rosana; Suzuki, Erika

    2017-04-01

    Paracoccidioides brasiliensis is one of the etiological agents of the human systemic mycosis paracoccidioidomycosis. Protease-activated receptors (PARs) are expressed in many cell types and comprise a family of G protein-coupled receptors (PAR-1, PAR-2, and PAR-4), which may be activated by proteases secreted by several pathogens. In the present study, we showed that the pathogenic fungus P. brasiliensis secretes components that promote interleukin (IL)-6 and IL-8 secretion by the lung epithelial cell line A549. Cytokine secretion was reduced by antagonistic peptides for PAR-1 and PAR-2, but not for PAR-4. P. brasiliensis proteases were isolated from fungal culture supernatants in a p-aminomethylbenzamidine-Sepharose column. The obtained fractions were tested for enzymatic activity against fluorescence resonance energy transfer (FRET) peptides derived from sequences that spanned the activation sites of human PARs. The eluted fraction, termed PbP, contained protease activities that were able to hydrolyze the FRET peptides. PbP also induced IL-6 and IL-8 secretion in A549 epithelial cells, which was reduced upon heat inactivation of PbP, incubation with antagonistic peptides for PAR-1 and PAR-2, and the protease inhibitors aprotinin, leupeptin, and E-64. Together, these results show for the first time that P. brasiliensis yeasts secrete proteases that activate PARs in lung epithelial cells, leading to cytokine secretion.

  5. Características morfológicas da pars de C2 de humanos Características morfológicas de la pars de C2 de humanos Morphologic characteristics of C2 humans pars

    Directory of Open Access Journals (Sweden)

    Charles André Carazzo

    2009-09-01

    Full Text Available OBJETIVO: avaliar a morfometria da pars da segunda vértebra cervical, verificar as variações existentes e predizer sua segurança para a colocação de parafusos por meio de uma abordagem cervical posterior. MÉTODOS: 58 vértebras foram obtidas para estudo anatômico (116 pars de C2 e foram medidas a altura e largura da pars, além do comprimento eixo-pedículo, largura e comprimento do pedículo, angulação sagital e medial. Todas as medidas foram separadas quanto aos seus lados direito e esquerdo; foi realizado o cálculo da média, variação, desvio padrão e a médica foi comparada com os estudos prévios. RESULTADOS: a largura média da pars de C2 foi de 9,5 mm; a altura, 9,5 mm; não houve diferença significativa entre os lados esquerdo e direito. O comprimento médio do pedículo foi de 10,5 mm; a medida média encontrada para o comprimento eixo-pedículo foi de 24,9 mm; a largura do pedículo foi de 2,8 mm. A angulação sagital encontrada foi de 19,6º e a horizontal foi de 28,5º. CONCLUSÕES: este estudo demonstra a viabilidade para a colocação de parafuso na pars de C2 em uma população caucasiana, destacando-se, porém, a grande variabilidade anatômica encontrada nas referidas medidas.OBJETIVO: evaluar la morfometría de la pars de la segunda vértebra cervical, verificar las variaciones existentes y predecir su seguridad para la colocación de los tornillos por un abordaje cervical posterior. MÉTODOS: 58 vértebras fueron obtenidas para el estudio anatómico (116 pars de C2 y fueron medidas su altura y el ancho de las pars, además de la largura eje-pedículo, ancho y largura del pedículo, ángulo sagital y medial. Todas las medidas fueron separadas según sus lados derecho e izquierdo, se realizó el cálculo de la media, variación, desvío estándar y comparados los valores con estudios previos. RESULTADOS: el ancho promedio de la pars de C2 fue de 9.5 mm, la altura de 9.5 mm, sin diferencia significativa entre los

  6. ParFit: A Python-Based Object-Oriented Program for Fitting Molecular Mechanics Parameters to ab Initio Data.

    Science.gov (United States)

    Zahariev, Federico; De Silva, Nuwan; Gordon, Mark S; Windus, Theresa L; Dick-Perez, Marilu

    2017-03-27

    A newly created object-oriented program for automating the process of fitting molecular-mechanics parameters to ab initio data, termed ParFit, is presented. ParFit uses a hybrid of deterministic and stochastic genetic algorithms. ParFit can simultaneously handle several molecular-mechanics parameters in multiple molecules and can also apply symmetric and antisymmetric constraints on the optimized parameters. The simultaneous handling of several molecules enhances the transferability of the fitted parameters. ParFit is written in Python, uses a rich set of standard and nonstandard Python libraries, and can be run in parallel on multicore computer systems. As an example, a series of phosphine oxides, important for metal extraction chemistry, are parametrized using ParFit. ParFit is in an open source program available for free on GitHub ( https://github.com/fzahari/ParFit ).

  7. The superoxide scavenger TEMPOL induces urokinase receptor (uPAR expression in human prostate cancer cells

    Directory of Open Access Journals (Sweden)

    Francis Joseph

    2006-06-01

    Full Text Available Abstract There is little understanding of the effect that reactive oxygen metabolites have on cellular behavior during the processes of invasion and metastasis. These oxygen metabolites could interact with a number of targets modulating their function such as enzymes involved in basement membrane dissolution, adhesion molecules involved in motility or receptors involved in proliferation. We investigated the effect of increased scavenging of superoxide anions on the expression of the urokinase receptor (uPAR in PC-3M human prostate cancer cells. Urokinase receptor is a GPI-linked cell surface molecule which mediates multiple functions including adhesion, proliferation and pericellular proteolysis. Addition of the superoxide scavenger 4-hydroxy-2,2,6,6-tetramethylpiperidinyloxy (TEMPOL to PC-3M cultures stimulated expression of uPAR protein peaking between 48 and 72 hours. Cell surface expression of the uPAR was also increased. Surprisingly, uPAR transcript levels increased only slightly and this mild increase did not coincide with the striking degree of protein increase. This disparity indicates that the TEMPOL effect on uPAR occurs through a post-transcriptional mechanism. TEMPOL presence in PC-3M cultures reduced intracellular superoxide-type species by 75% as assayed by NBT dye conversion; however this reduction significantly diminished within hours following TEMPOL removal. The time gap between TEMPOL treatment and peak uPAR protein expression suggests that reduction of reactive oxygen metabolites in prostate cancer cells initiates a multistep pathway which requires several hours to culminate in uPAR induction. These findings reveal a novel pathway for uPAR regulation involving reactive oxygens such as superoxide anion.

  8. Expression of guanylin in “pars tuberalis-specific cells” and gonadotrophs of rat adenohypophysis‖

    OpenAIRE

    D'Este, Loredana; Kulaksiz, Hasan; Rausch, Ulrich; Vaccaro, Rosa; Wenger, Tibor; Tokunaga, Yoshimitsu; Renda, Tindaro G.; Cetin, Yalcin

    2000-01-01

    The intestinal peptide guanylin regulates the electrolyte/water transport in the gastrointestinal epithelium by paracrine/luminocrine mechanisms. Because guanylin also circulates in the blood, we investigated the rat hypothalamo-pituitary region for expression and cellular localization of this peptide. Reverse transcriptase–PCR analyses with guanylin-specific primers revealed expression of the peptide in the pars tuberalis and pars distalis of the pituitary. Western blotting analyses in hypop...

  9. Contamination du sol, de l'eau et des produits maraîchers par des ...

    African Journals Online (AJOL)

    ... de l'eau et des produits maraîchers par des substances toxiques et des métaux ... L'usage non maîtrisé d'intrants agricoles et de pesticides a des effets sur la ... maraîchères sont contaminées par des métaux lourds notamment le plomb, ...

  10. Tsitutu , ou mecanisme local de mediation et de reconciliation « par ...

    African Journals Online (AJOL)

    Si le Togo est une nation pluriethnique, du coup, il y en aura des manières particulières de résolution des conflits, donc des réconciliations. Par une approche pluridisciplinaire, cette étude a pour but de restituer le mécanisme local de réconciliation chez les ewe de Kloto. Par ailleurs, ce rituel qui tente de proposer des ...

  11. Endophthalmitis Due to Proteus vulgaris after Pars Plana Vitrectomy with Devastating Outcome.

    Science.gov (United States)

    Al-Kharashi, Abdullah S; Al-Kharshi, Abdulmajeed S; Al-Faky, Yasser H

    2017-01-01

    Postoperative infectious endophthalmitis is rare, yet devastating loss of vision or loss of the eye can occur due to a highly purulent microorganism or uncontrolled endophthalmitis that may spread to all coats of the eye. We report, herewith, a case of rapidly progressive postoperative endophthalmitis after pars plana vitrectomy which ended up with enucleation. The isolated microorganism was Proteus vulgaris which has not been reported as causative bacteria of postoperative infections following pars plana vitrectomy.

  12. 27 Segmentation par logique floue pour l'estimation du nombre de ...

    African Journals Online (AJOL)

    bagui kossan olivier

    modes, par un ensemble de 13 DELs, avec des longueurs d'onde allant de 375nm à 940nm. Une source en ... (13 images par mode), pour la même scène, en utilisant une carte d'acquisition de données (NI-DAQ) couplée ..... malaria diagnostics by multispectral and multimodality light-emitting-diode microscopy. Journal of.

  13. Anatomic relationship between the anterior sylvian point and the pars triangularis.

    Science.gov (United States)

    Ayberk, Giyas; Yagli, O Emre; Comert, Ayhan; Esmer, Ali Firat; Canturk, Nergis; Tekdemir, Ibrahim; Dinc, Hakan

    2012-05-01

    The aim of this study was to show morphological sulcal variations of the pars triangularis of the inferior frontal gyrus and to provide a clearer description of the anterior sylvian point. Thirty-six hemispheres of 18 adult cadavers were studied. The hemispheres were harvested by the classical autopsy method and fixed in 10% formalin solution for three weeks. In six hemispheres, the arteries and veins were filled with colored silicone. The proximal and distal segments of the sylvian fissure, the perpendicular distance of both the anterior sylvian point and inferior rolandic point to the insular cortex and the distances between the anterior ascending ramus and the precentral, central, and postcentral sulcus were measured. The anterior horizontal and ascending rami were exposed. The sulcus located on the pars triangularis was appraised. The relationship between the anterior sylvian point and the vascular structure around the sylvian fissure was examined. The rising of the anterior horizontal and ascending ramus from the sylvian fissure defines the shape of the pars triangularis. The pars triangularis has three shapes: V, U, and Y. In V- and Y-shaped pars triangularis both rami merge but in U-shaped pars triangularis the rami do not merge. The pars triangularis was Y-shaped in 30.76% (4/13) of the right hemispheres and in 50% (7/14) of the left hemispheres; U-shaped in 20.3% (3/13) of the right hemispheres and in 35.71% (5/14) of the left hemispheres; V-shaped in 40.61% (6/13) of the right hemispheres and in 14.29% (2/14) of the left hemispheres. Minimally invasive procedures use basic anatomic landmarks intracranially to reach the targeted area; therefore, exact and detailed knowledge of the anatomy of the sylvian fissure and pars triangularis is of great importance. Copyright © 2011 Wiley Periodicals, Inc.

  14. Les corps etrangers des fosses nasales: Extraction par la pince d ...

    African Journals Online (AJOL)

    Les corps etrangers des fosses nasales: Extraction par la pince d'halsteaD. ... des fosses nasales: Extraction par la pince d'halsteaD. B Amana, E Kpemissi, P Agoda, A Naba-Mouchedou ... Les corps étrangers des fosses nasales représentent 0,94 % des consultations ORL du service. L'âge des patients a varié de 9 mois à ...

  15. Etude par simulation hil des performances d'un statcom pour la ...

    African Journals Online (AJOL)

    Ce travail présente l'étude par simulation Hardware In the Loop (HIL) des performances d'un STATCOM pour la stabilisation de la tension délivrée par une génératrice asynchrone triphasée auto excitée dans un réseau autonome. Nous avons combiné l'utilisation du logiciel de simulation LABVIEW et la carte ARDUINO ...

  16. Molecular mechanism of bundle formation by the bacterial actin ParM

    Energy Technology Data Exchange (ETDEWEB)

    Popp, David, E-mail: dpopp@imcb.a-star.edu.sg [ERATO ' Actin Filament Dynamics' Project, Japan Science and Technology Corporation, c/o RIKEN Harima Institute at Spring 8, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan); Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, 138673 Singapore (Singapore); Narita, Akihiro [ERATO ' Actin Filament Dynamics' Project, Japan Science and Technology Corporation, c/o RIKEN Harima Institute at Spring 8, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan); Nagoya University Graduate School of Science, Structural Biology Research Center and Division of Biological Sciences, Furo-cho, Chikusa-ku, Nagoya 464-8601 (Japan); Iwasa, Mitsusada [ERATO ' Actin Filament Dynamics' Project, Japan Science and Technology Corporation, c/o RIKEN Harima Institute at Spring 8, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan); Maeda, Yuichiro [ERATO ' Actin Filament Dynamics' Project, Japan Science and Technology Corporation, c/o RIKEN Harima Institute at Spring 8, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan); Nagoya University Graduate School of Science, Structural Biology Research Center and Division of Biological Sciences, Furo-cho, Chikusa-ku, Nagoya 464-8601 (Japan); Robinson, Robert C. [Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, 138673 Singapore (Singapore)

    2010-01-22

    The actin homolog ParM plays a microtubule-like role in segregating DNA prior to bacterial cell division. Fluorescence and cryo-electron microscopy have shown that ParM forms filament bundles between separating DNA plasmids in vivo. Given the lack of ParM bundling proteins it remains unknown how ParM bundles form at the molecular level. Here we show using time-lapse TIRF microscopy, under in vitro molecular crowding conditions, that ParM-bundle formation consists of two distinct phases. At the onset of polymerization bundle thickness and shape are determined in the form of nuclei of short helically disordered filaments arranged in a liquid-like lattice. These nuclei then undergo an elongation phase whereby they rapidly increase in length. At steady state, ParM bundles fuse into one single large aggregate. This behavior had been predicted by theory but has not been observed for any other cytomotive biopolymer, including F-actin. We employed electron micrographs of ParM rafts, which are 2-D analogs of 3-D bundles, to identify the main molecular interfilament contacts within these suprastructures. The interface between filaments is similar for both parallel and anti-parallel orientations and the distribution of filament polarity is random within a bundle. We suggest that the interfilament interactions are not due to the interactions of specific residues but rather to long-range, counter ion mediated, electrostatic attractive forces. A randomly oriented bundle ensures that the assembly is rigid and that DNA may be captured with equal efficiency at both ends of the bundle via the ParR binding protein.

  17. La décoloration des rejets liquides de textile (noir de soufre) par ...

    African Journals Online (AJOL)

    Dans cette étude, nous nous intéressons au traitement d'un rejet de coloration de textile par voie électrochimique. Les paramètres qui ont été variés au cours de traitement par cette technique sont le pourcentage de dilution et le courant imposé. Les résultats obtenus ont permis d'obtenir un taux de décoloration pouvant ...

  18. The RNA-binding protein ATX-2 regulates cytokinesis through PAR-5 and ZEN-4.

    Science.gov (United States)

    Gnazzo, Megan M; Uhlemann, Eva-Maria E; Villarreal, Alex R; Shirayama, Masaki; Dominguez, Eddie G; Skop, Ahna R

    2016-10-15

    The spindle midzone harbors both microtubules and proteins necessary for furrow formation and the completion of cytokinesis. However, the mechanisms that mediate the temporal and spatial recruitment of cell division factors to the spindle midzone and midbody remain unclear. Here we describe a mechanism governed by the conserved RNA-binding protein ATX-2/Ataxin-2, which targets and maintains ZEN-4 at the spindle midzone. ATX-2 does this by regulating the amount of PAR-5 at mitotic structures, particularly the spindle, centrosomes, and midbody. Preventing ATX-2 function leads to elevated levels of PAR-5, enhanced chromatin and centrosome localization of PAR-5-GFP, and ultimately a reduction of ZEN-4-GFP at the spindle midzone. Codepletion of ATX-2 and PAR-5 rescued the localization of ZEN-4 at the spindle midzone, indicating that ATX-2 mediates the localization of ZEN-4 upstream of PAR-5. We provide the first direct evidence that ATX-2 is necessary for cytokinesis and suggest a model in which ATX-2 facilitates the targeting of ZEN-4 to the spindle midzone by mediating the posttranscriptional regulation of PAR-5. © 2016 Gnazzo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  19. Willin and Par3 cooperatively regulate epithelial apical constriction through aPKC-mediated ROCK phosphorylation.

    Science.gov (United States)

    Ishiuchi, Takashi; Takeichi, Masatoshi

    2011-06-19

    Apical-domain constriction is important for regulating epithelial morphogenesis. Epithelial cells are connected by apical junctional complexes (AJCs) that are lined with circumferential actomyosin cables. The contractility of these cables is regulated by Rho-associated kinases (ROCKs). Here, we report that Willin (a FERM-domain protein) and Par3 (a polarity-regulating protein) cooperatively regulate ROCK-dependent apical constriction. We found that Willin recruits aPKC and Par6 to the AJCs, independently of Par3. Simultaneous depletion of Willin and Par3 completely removed aPKC and Par6 from the AJCs and induced apical constriction. Induced constriction was through upregulation of the level of AJC-associated ROCKs, which was due to loss of aPKC. Our results indicate that aPKC phosphorylates ROCK and suppresses its junctional localization, thereby allowing cells to retain normally shaped apical domains. Thus, we have uncovered a Willin/Par3-aPKC-ROCK pathway that controls epithelial apical morphology.

  20. Examining relational empowerment for elementary school students in a yPAR program.

    Science.gov (United States)

    Langhout, Regina Day; Collins, Charles; Ellison, Erin Rose

    2014-06-01

    This paper joins relational empowerment, youth empowerment, and Bridging Multiple Worlds frameworks to examine forms of relational empowerment for children in two intermediary institutions-school and a youth participatory action research after-school program (yPAR ASP). Participants were twelve children, most of whom were Latina/o and from im/migrant families, enrolled in a yPAR ASP for 2 years. A mixed-method approach was utilized; we analyzed children's interviews, self-defined goals, and their social networks to examine their experiences of relational empowerment. We conclude that children experienced each of the five relational empowerment factors-collaborative competence, bridging social divisions, facilitating others' empowerment, mobilizing networks, and passing on a legacy-in the yPAR ASP setting, and some factors in school. These experiences, however, were more pronounced in the yPAR ASP setting. Additionally, social network analyses revealed that a small but meaningful percentage of actors bridged worlds, especially home and family, but by year 2, also school and the yPAR ASP. Finally, most helpers for school-based goals came from school, but a sizable number came from family, friends, and home worlds, and by year 2, also came from the yPAR ASP. Implications range from theoretical to methodological development, including the use of social network analysis as a tool to descriptively examine relational power in context.

  1. Evaluation of orthodontists' perception of treatment need and the peer assessment rating (PAR) index.

    Science.gov (United States)

    McGorray, S P; Wheeler, T T; Keeling, S D; Yurkiewicz, L; Taylor, M G; King, G J

    1999-08-01

    This paper examines the relationship between orthodontists' subjective assessment of treatment need and objective measurements obtained during standardized intra- and extraoral examinations. Logistic regression modeling was used to develop predictive models of treatment need. Data were obtained from 1155 eighth-grade students by four orthodontists who used standardized examination forms to assess demographics, trauma, skeletal relationships, morphologic malocclusion traits, and mandibular function. At the conclusion of the examination, the orthodontist rated the subjective treatment need as none, elective, recommended, soon, or immediate. For some analyses, the categories were collapsed to represent no need and need. The peer assessment rating (PAR) index (American validated version) was computed from the clinical exam findings and scoring of dental models; PAR scores were used to document malocclusion severity and treatment difficulty. Spearman rank correlation coefficients quantified the relationship between PAR scores and need categories. Logistic regression analysis modeled treatment need using components of the PAR index as well as other variables. The components of these models, as well as sensitivity and specificity, were compared with malocclusion severity/treatment difficulty scores obtained from malocclusion assessments using the PAR index. The five subjective treatment need categories and the PAR index scores were significantly correlated (rho = 0.62, porthodontists' subjective assessment of treatment need when that assessment is made in the absence of financial considerations and patient desires.

  2. Les déterminants de la migration des clients entre les marques nationales et les marques de distributeurs

    OpenAIRE

    Ramaroson, Andry Haja

    2009-01-01

    For the last years, the consistent development of store brands or private labels has resulted in a market composed of three segments: customers who are national brand loyal, (ii) store brand loyal and (iii) those who combine store and national brands. So the question becomes: why does the latter group of consumers migrate from a national brand to a store brand and vice versa? To our knowledge, no study has been conducted about this aspect of national brand and private label competition. The r...

  3. Clinical relevance and cellular source of elevated soluble urokinase plasminogen activator receptor (suPAR) in acute liver failure.

    Science.gov (United States)

    Koch, Alexander; Zimmermann, Henning W; Gassler, Nikolaus; Jochum, Christoph; Weiskirchen, Ralf; Bruensing, Jan; Buendgens, Lukas; Dückers, Hanna; Bruns, Tony; Gerken, Guido; Neumann, Ulf P; Adams, David H; Trautwein, Christian; Canbay, Ali; Tacke, Frank

    2014-10-01

    Acute liver failure (ALF) is a life-threatening condition with a high mortality rate. The expression of urokinase plasminogen activator receptor (uPAR, CD87) and release of its shedded receptor into serum as soluble uPAR (suPAR) have been closely related to immune activation and prognosis in systemic inflammation and cirrhosis. We now aimed at investigating the clinical relevance and cellular source of uPAR and circulating suPAR in ALF. Serum suPAR concentrations were measured in 48 ALF patients and 62 healthy controls from a German liver transplantation centre. Hepatic immune cell subsets and uPAR expression were studied by FACS, qPCR and immunohistochemistry. Circulating suPAR levels were significantly increased in ALF patients, independent from the underlying aetiology, in comparison to controls. Serum suPAR concentrations were closely correlated with parameters reflecting liver cell injury, decreased liver function and the model of end-stage liver disease (MELD) score in ALF patients. By immunohistochemistry from explanted livers, ALF was associated with distinct immune cell accumulation and strong up-regulation of intrahepatic uPAR mRNA expression. CD87 (uPAR) expression was specifically detected on intrahepatic 'non-classical' monocytes (CD14(+) CD16(+) ), NKT and CD56(dim) NK cells isolated from human liver, but not on parenchymal or other non-parenchymal hepatic cell types. Membrane-bound uPAR was rapidly cleaved from monocytes upon inflammatory stimulation by lipopolysaccharide (LPS) and partially by co-cultured lymphocytes. Similar to its prognostic properties in patients with sepsis or cirrhosis, intrahepatic uPAR activation and serum suPAR concentrations might serve as an interesting biomarker in ALF. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  4. Etiología en parálisis cerebral.

    Directory of Open Access Journals (Sweden)

    Patricia Campos

    1996-07-01

    Full Text Available Objetivo: Determinar las causas mas frecuentes de parálisis cerebral (PC. Material y métodos: Estudio descriptivo realizado en Hospital Nacional Cayetano Heredia y el Hogar Clínica San Juan de Dios (Lima-Perú, entre noviembre 1990 y mayo de 1992. Se revisaron las historias clínicas de 102 pacientes con diagnósticos de PC y se revisó las etiologías. Resultados: En relación a edad gestacional hubieron 15 casos de prematuros (14.7%, 67(65% a término y un postmaduro, en 19 no se tuvo la edad gestacional. El peso al nacer fue adecuado en 56(54.9%, bajo en 17(16.7% y muy bajo en 7(6.9%. Se identificó la causa de PC en 73/102 casos (70%, siendo las causas más frecuentes las perinatales (59.3%, seguidas de las prenatales con 29.1% y las postnatales con 11.6%. En el grupo de causas prenatales, la mitad fueron malformaciones cerebrales congénitas entre las que destaca el quiste porencefálico, presente en la mitad de estos casos. En el grupo perinatal, la más frecuente fue asfixia (83% asociada o no a otro factor metabólico. Hubieron además en este grupo 4 casos de hipoglicemia severa y 2 con encefalopatía hiperbilirrubinémica y PC distónica. Las causas postnatal más frecuente fue la meningoencefalitis bacteriana, en menores de un año de edad. Al correlacionar etiología y momento del daño, peso al nacer y tipo de PC se observa que, en los recién nacidos PT de bajo o muy bajo peso al nacer es mas frecuente la PC de tipo dipléjica y en todos el antecedente fue asfixia perinatal. Conclusiones: La baja frecuencia de PC dipléjica y la elevada frecuencia de causas perinatales (asfixia, marca nuestra diferencia con otras series y obedece en nuestra opinión, a los escasos recursos tecnológicos y las deficiencias en la atención a las gestantes y recién nacidos de nuestro sistema nacional de salud. (Rev Med Hered 1996; 7: 114-118.

  5. Detection of airborne Par j 1 and Par j 2 allergens in relation to Urticaceae pollen counts in different bioclimatic areas.

    Science.gov (United States)

    Jato, Victoria; Rodríguez-Rajo, F Javier; González-Parrado, Zulima; Elvira-Rendueles, Belén; Moreno-Grau, Stella; Vega-Maray, Ana; Fernández-González, Delia; Asturias, Juan A; Suárez-Cervera, María

    2010-07-01

    In aerobiological studies, the Parietaria pollen type usually includes all Parietaria and Urtica species found in the area. Given that Urtica is a nonallergenic plant, the pollen counts report incomplete information on the presence of allergens in the atmosphere. Discordance between the pollen concentrations of Urticaceae and allergic symptoms has been observed in patients with pollinosis. To compare the Urticaceae pollen counts with the Par j 1 and Par j 2 aeroallergen concentrations from 2 different Spanish geographic areas to determine the allergenic load in the atmosphere. Hirst-type volumetric traps and Burkard Cyclone samplers were used for pollen counts and aeroallergen capture, respectively. The quantification of Par j 1 and Par j 2 allergens was performed using specific 2-site antibody enzyme-linked immunosorbent assay. Transmission electron microscopy and immunocytochemical techniques were applied to localize these allergens in the orbicules. Differences between areas and years were obtained in both pollen and aeroallergen concentrations. Despite the lower pollen counts recorded in Cartagena, higher aeroallergen concentrations were registered compared with Ourense. A lower correlation was achieved between Urticaceae pollen concentrations and aeroallergen levels, with a maximum positive significant correlation (adjusted R2 = 0.466, P Parietaria aeroallergens and clinical symptoms. Therefore, the measurement of aeroallergens should be incorporated into the aerobiological studies with clinical applications.

  6. Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA

    Science.gov (United States)

    Minnen, Anita; Bürmann, Frank; Wilhelm, Larissa; Anchimiuk, Anna; Diebold-Durand, Marie-Laure; Gruber, Stephan

    2016-01-01

    Summary Smc/ScpAB promotes chromosome segregation in prokaryotes, presumably by compacting and resolving nascent sister chromosomes. The underlying mechanisms, however, are poorly understood. Here, we investigate the role of the Smc ATPase activity in the recruitment of Smc/ScpAB to the Bacillus subtilis chromosome. We demonstrate that targeting of Smc/ScpAB to ParB/parS loading sites is strictly dependent on engagement of Smc head domains and relies on an open organization of the Smc coiled coils. We find that dimerization of the Smc hinge domain stabilizes closed Smc rods and hinders head engagement as well as chromosomal targeting. Conversely, the ScpAB sub-complex promotes head engagement and Smc rod opening and thereby facilitates recruitment of Smc to parS sites. Upon ATP hydrolysis, Smc/ScpAB is released from loading sites and relocates within the chromosome—presumably through translocation along DNA double helices. Our findings define an intermediate state in the process of chromosome organization by Smc. PMID:26904953

  7. Heat stress-induced disruption of endothelial barrier function is via PAR1 signaling and suppressed by Xuebijing injection.

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    Qiulin Xu

    Full Text Available Increased vascular permeability leading to acute lung injury (ALI and acute respiratory distress syndrome (ARDS is central to the pathogenesis of heatstroke. Protease-activated receptor 1 (PAR1, the receptor for thrombin, plays a key role in disruption of endothelial barrier function in response to extracellular stimuli. However, the role of PAR1 in heat stress-induced endothelial hyper-permeability is unknown. In this study, we measured PAR1 protein expression in heat-stressed human umbilical venous endothelial cells (HUVECs, investigated the influences of PAR1 on endothelial permeability, F-actin rearrangement, and moesin phosphorylation by inhibiting PAR1 with its siRNA, neutralizing antibody (anti-PAR1, specific inhibitor(RWJ56110, and Xuebijing injection (XBJ, a traditional Chinese medicine used for sepsis treatment, and evaluated the role of PAR1 in heatstroke-related ALI/ARDS in mice by suppressing PAR1 with RWJ56110, anti-PAR1and XBJ. We found that heat stress induced PAR1 protein expression 2h after heat stress in endothelial cells, caused the release of endothelial matrix metalloprotease 1, an activator of PAR1, after 60 or 120 min of heat stimulation, as well as promoted endothelial hyper-permeability and F-actin rearrangement, which were inhibited by suppressing PAR1 with RWJ56110, anti-PAR1 and siRNA. PAR1 mediated moesin phosphorylation, which caused F-actin rearrangement and disruption of endothelial barrier function. To corroborate findings from in vitro experiments, we found that RWJ56110 and the anti-PAR1 significantly decreased lung edema, pulmonary microvascular permeability, protein exudation, and leukocytes infiltrations in heatstroke mice. Additionally, XBJ was found to suppress PAR1-moesin signal pathway and confer protective effects on maintaining endothelial barrier function both in vitro and in vivo heat-stressed model, similar to those observed above with the inhibition of PAR1. These results suggest that PAR1 is a

  8. A method for estimating the diffuse attenuation coefficient (KdPAR)from paired temperature sensors

    Science.gov (United States)

    Read, Jordan S.; Rose, Kevin C.; Winslow, Luke A.; Read, Emily K.

    2015-01-01

    A new method for estimating the diffuse attenuation coefficient for photosynthetically active radiation (KdPAR) from paired temperature sensors was derived. We show that during cases where the attenuation of penetrating shortwave solar radiation is the dominant source of temperature changes, time series measurements of water temperatures at multiple depths (z1 and z2) are related to one another by a linear scaling factor (a). KdPAR can then be estimated by the simple equation KdPAR ln(a)/(z2/z1). A suggested workflow is presented that outlines procedures for calculating KdPAR according to this paired temperature sensor (PTS) method. This method is best suited for conditions when radiative temperature gains are large relative to physical noise. These conditions occur frequently on water bodies with low wind and/or high KdPARs but can be used for other types of lakes during time periods of low wind and/or where spatially redundant measurements of temperatures are available. The optimal vertical placement of temperature sensors according to a priori knowledge of KdPAR is also described. This information can be used to inform the design of future sensor deployments using the PTS method or for campaigns where characterizing sub-daily changes in temperatures is important. The PTS method provides a novel method to characterize light attenuation in aquatic ecosystems without expensive radiometric equipment or the user subjectivity inherent in Secchi depth measurements. This method also can enable the estimation of KdPAR at higher frequencies than many manual monitoring programs allow.

  9. Detection of pars injury by SPECT in patients younger than age 10 with low back pain.

    Science.gov (United States)

    Spencer, Hillard T; Sokol, Levi O; Glotzbecker, Michael P; Grant, Frederick D; d'Hemecourt, Pierre A; Micheli, Lyle J; Treves, S Ted

    2013-06-01

    Evaluation of extension-based low back pain in young athletes with suspected pars injury may include a referral for skeletal single photon emission computed tomography (SPECT). However, the diagnostic yield of this technique in children with low back pain before the age of 10 years remains uncertain. We examined a series of consecutive SPECT scans to address this question. A retrospective review of department databases revealed 107 consecutive skeletal Tc-99m MDP SPECT scans performed between January 1, 2007 and December 31, 2009 in children less than 10 years of age. Of these, 72 studies were performed for a referral diagnosis of back pain. There were 43 girls (44 studies) and 28 boys (28 studies). The mean age was 7.2 years (range, 1.9 to 9.9 y). All SPECT scans were reviewed and positive findings documented. In addition, all available anatomic imaging, imaging reports (computed tomography, magnetic resonance, and x-ray) and clinical notes were reviewed, and results were compared with those of SPECT studies. Of the 72 SPECT studies, 35 (49%) identified a focal area in the spine of abnormal increased uptake, with 17 in the region of the pars interarticularis. With additional imaging, 1 case was demonstrated not to be a pars injury (computed tomography showed a transverse process fracture) and 2 patients with negative SPECT scans were shown to have pars injuries that SPECT scan had not detected, for a total of 18 pars injuries (25%) in this cohort. Reported participation in gymnastics or football was related to pars injury (odds ratio 4.3, P=0.04). Pars injury was found in 25% of children referred for SPECT scan with back pain below 10 years of age. SPECT scan was highly sensitive for this injury as well as in identifying other potential sites of pathology, and should be considered in the workup of persistent low back pain in young children. Level II, diagnostic study.

  10. ParsEval: parallel comparison and analysis of gene structure annotations

    Directory of Open Access Journals (Sweden)

    Standage Daniel S

    2012-08-01

    Full Text Available Abstract Background Accurate gene structure annotation is a fundamental but somewhat elusive goal of genome projects, as witnessed by the fact that (model genomes typically undergo several cycles of re-annotation. In many cases, it is not only different versions of annotations that need to be compared but also different sources of annotation of the same genome, derived from distinct gene prediction workflows. Such comparisons are of interest to annotation providers, prediction software developers, and end-users, who all need to assess what is common and what is different among distinct annotation sources. We developed ParsEval, a software application for pairwise comparison of sets of gene structure annotations. ParsEval calculates several statistics that highlight the similarities and differences between the two sets of annotations provided. These statistics are presented in an aggregate summary report, with additional details provided as individual reports specific to non-overlapping, gene-model-centric genomic loci. Genome browser styled graphics embedded in these reports help visualize the genomic context of the annotations. Output from ParsEval is both easily read and parsed, enabling systematic identification of problematic gene models for subsequent focused analysis. Results ParsEval is capable of analyzing annotations for large eukaryotic genomes on typical desktop or laptop hardware. In comparison to existing methods, ParsEval exhibits a considerable performance improvement, both in terms of runtime and memory consumption. Reports from ParsEval can provide relevant biological insights into the gene structure annotations being compared. Conclusions Implemented in C, ParsEval provides the quickest and most feature-rich solution for genome annotation comparison to date. The source code is freely available (under an ISC license at http://parseval.sourceforge.net/.

  11. uPA/uPAR system activation drives a glycolytic phenotype in melanoma cells.

    Science.gov (United States)

    Laurenzana, Anna; Chillà, Anastasia; Luciani, Cristina; Peppicelli, Silvia; Biagioni, Alessio; Bianchini, Francesca; Tenedini, Elena; Torre, Eugenio; Mocali, Alessandra; Calorini, Lido; Margheri, Francesca; Fibbi, Gabriella; Del Rosso, Mario

    2017-09-15

    In this manuscript, we show the involvement of the uPA/uPAR system in the regulation of aerobic glycolysis of melanoma cells. uPAR over-expression in human melanoma cells controls an invasive and glycolytic phenotype in normoxic conditions. uPAR down-regulation by siRNA or its uncoupling from integrins, and hence from integrin-linked tyrosine kinase receptors (IL-TKRs), by an antagonist peptide induced a striking inhibition of the PI3K/AKT/mTOR/HIF1α pathway, resulting into impairment of glucose uptake, decrease of several glycolytic enzymes and of PKM2, a checkpoint that controls metabolism of cancer cells. Further, binding of uPA to uPAR regulates expression of molecules that govern cell invasion, including extracellular matrix metallo-proteinases inducer (EMPPRIN) and enolase, a glycolytyc enzyme that also serves as a plasminogen receptor, thus providing a common denominator between tumor metabolism and phenotypic invasive features. Such effects depend on the α5β1-integrin-mediated uPAR connection with EGFR in melanoma cells with engagement of the PI3K-mTOR-HIFα pathway. HIF-1α trans-activates genes whose products mediate tumor invasion and glycolysis, thus providing the common denominator between melanoma metabolism and its invasive features. These findings unveil a unrecognized interaction between the invasion-related uPAR and IL-TKRs in the control of glycolysis and disclose a new pharmacological target (i.e., uPAR/IL-TKRs axis) for the therapy of melanoma. © 2017 UICC.

  12. Lumbar lordosis and pars interarticularis fractures: a case-control study

    Energy Technology Data Exchange (ETDEWEB)

    Bugg, William G.; Lewis, Mark; Juette, Arne; Cahir, John G.; Toms, Andoni P. [Cotman Centre, Norwich Radiology Academy, Norwich, Norfolk (United Kingdom)

    2012-07-15

    The aim of this study is to examine the relationship between lumbar lordosis and pars interarticularis fractures. In this retrospective case-control study we compare the angle of lumbar lordosis and the angle of the S1 vertebral endplate (as a measure of pelvic tilt) in patients with bilateral L5 pars interarticularis fractures with age- and sex-matched control cases with normal MRI examinations of the lumbar spine. Twenty-nine cases of bilateral L5 pars interarticularis fractures with matched control-cases were identified on MRI (16 male, 13 female, age 9-63 years). The angle of lordosis was measured between the inferior L4 and superior S1 vertebral endplates on a standing lateral lumbar spine radiograph for both groups. The mean angle of lordosis about the L5 vertebra was 36.9 (SD = 6.5 ) in the pars interarticularis fracture group, and 30.1 (SD = 6.4 ) in the control group. The difference between the two groups was significant (mean difference 6.8 , Student's t test: P < 0.001). The mean angle of sacral tilt measured was 122.2 (SD = 10.16 ) for controls and 136.4 (SD = 10.86 ) for patients with pars defects. The difference in the means of 14.2 was statistically significantly different (P < 0.0001). Sacral tilt represented by a steeply angled superior endplate of S1 is associated with a significantly increased angle of lordosis, between L4 and S1, and pars fractures at L5. Steep angulation of the first sacral vertebral segment maybe the predisposing biomechanical factor that leads to pincer-like impingement of the pars interarticularis and then spondylolysis. (orig.)

  13. Clathrin and LRP-1-independent constitutive endocytosis and recycling of uPAR.

    Directory of Open Access Journals (Sweden)

    Katia Cortese

    Full Text Available BACKGROUND: The urokinase receptor (uPAR/CD87 is highly expressed in malignant tumours. uPAR, as a GPI anchored protein, is preferentially located at the cell surface, where it interacts with its ligands urokinase (uPA and the extracellular matrix protein vitronectin, thus promoting plasmin generation, cell-matrix interactions and intracellular signalling events. Interaction with a complex formed by uPA and its inhibitor PAI-1 induces cell surface down regulation and recycling of the receptor via the clathrin-coated pathway, a process dependent on the association to LRP-1. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we have found that along with the ligand-induced down-regulation, uPAR also internalizes and recycles constitutively through a second pathway that is independent of LRP-1 and clathrin but shares some properties with macropinocytosis. The ligand-independent route is amiloride-sensitive, does not require uPAR partitioning into lipid rafts, is independent of the activity of small GTPases RhoA, Rac1 and Cdc42, and does not require PI3K activity. Constitutively endocytosed uPAR is found in EEA1 positive early/recycling endosomes but does not reach lysosomes in the absence of ligands. Electron microscopy analysis reveals the presence of uPAR in ruffling domains at the cell surface, in macropinosome-like vesicles and in endosomal compartments. CONCLUSIONS/SIGNIFICANCE: These results indicate that, in addition to the ligand-induced endocytosis of uPAR, efficient surface expression and membrane trafficking might also be driven by an uncommon macropinocytic mechanism coupled with rapid recycling to the cell surface.

  14. Identification of a new epitope in uPAR as a target for the cancer therapeutic monoclonal antibody ATN-658, a structural homolog of the uPAR binding integrin CD11b (αM.

    Directory of Open Access Journals (Sweden)

    Xiang Xu

    Full Text Available The urokinase plasminogen activator receptor (uPAR plays a role in tumor progression and has been proposed as a target for the treatment of cancer. We recently described the development of a novel humanized monoclonal antibody that targets uPAR and has anti-tumor activity in multiple xenograft animal tumor models. This antibody, ATN-658, does not inhibit ligand binding (i.e. uPA and vitronectin to uPAR and its mechanism of action remains unclear. As a first step in understanding the anti-tumor activity of ATN-658, we set out to identify the epitope on uPAR to which ATN-658 binds. Guided by comparisons between primate and human uPAR, epitope mapping studies were performed using several orthogonal techniques. Systematic site directed and alanine scanning mutagenesis identified the region of aa 268-275 of uPAR as the epitope for ATN-658. No known function has previously been attributed to this epitope Structural insights into epitope recognition were obtained from structural studies of the Fab fragment of ATN-658 bound to uPAR. The structure shows that the ATN-658 binds to the DIII domain of uPAR, close to the C-terminus of the receptor, corroborating the epitope mapping results. Intriguingly, when bound to uPAR, the complementarity determining region (CDR regions of ATN-658 closely mimic the binding regions of the integrin CD11b (αM, a previously identified uPAR ligand thought to be involved in leukocyte rolling, migration and complement fixation with no known role in tumor progression of solid tumors. These studies reveal a new functional epitope on uPAR involved in tumor progression and demonstrate a previously unrecognized strategy for the therapeutic targeting of uPAR.

  15. CFD modelling of hydrogen stratification in enclosures: Model validation and application to PAR performance

    Energy Technology Data Exchange (ETDEWEB)

    Hoyes, J.R., E-mail: james.hoyes@hsl.gsi.gov.uk; Ivings, M.J.

    2016-12-15

    Highlights: • The ability of CFD to predict hydrogen stratification phenomena is investigated. • Contrary to expectation, simulations on tetrahedral meshes under-predict mixing. • Simulations on structured meshes give good agreement with experimental data. • CFD model used to investigate the effects of stratification on PAR performance. • Results show stratification can have a significant effect on PAR performance. - Abstract: Computational Fluid Dynamics (CFD) models are maturing into useful tools for supporting safety analyses. This paper investigates the capabilities of CFD models for predicting hydrogen stratification in a containment vessel using data from the NEA/OECD SETH2 MISTRA experiments. Further simulations are then carried out to illustrate the qualitative effects of hydrogen stratification on the performance of Passive Autocatalytic Recombiner (PAR) units. The MISTRA experiments have well-defined initial and boundary conditions which makes them well suited for use in a validation study. Results are presented for the sensitivity to mesh resolution and mesh type. Whilst the predictions are shown to be largely insensitive to the mesh resolution they are surprisingly sensitive to the mesh type. In particular, tetrahedral meshes are found to induce small unphysical convection currents that result in molecular diffusion and turbulent mixing being under-predicted. This behaviour is not unique to the CFD model used here (ANSYS CFX) and furthermore, it may affect simulations run on other non-aligned meshes (meshes that are not aligned perpendicular to gravity), including non-aligned structured meshes. Following existing best practice guidelines can help to identify potential unphysical predictions, but as an additional precaution consideration should be given to using gravity-aligned meshes for modelling stratified flows. CFD simulations of hydrogen recombination in the Becker Technologies THAI facility are presented with high and low PAR positions

  16. PAR-1 mediated apoptosis of breast cancer cells by V. cholerae hemagglutinin protease.

    Science.gov (United States)

    Ray, Tanusree; Pal, Amit

    2016-05-01

    Bacterial toxins have emerged as promising agents in cancer treatment strategy. Hemagglutinin (HAP) protease secreted by Vibrio cholerae induced apoptosis in breast cancer cells and regresses tumor growth in mice model. The success of novel cancer therapies depends on their selectivity for cancer cells with limited toxicity for normal tissues. Increased expression of Protease Activated Receptor-1 (PAR-1) has been reported in different malignant cells. In this study we report that HAP induced activation and over expression of PAR-1 in breast cancer cells (EAC). Immunoprecipitation studies have shown that HAP specifically binds with PAR-1. HAP mediated activation of PAR-1 caused nuclear translocation of p50-p65 and the phosphorylation of p38 which triggered the activation of NFκB and MAP kinase signaling pathways. These signaling pathways enhanced the cellular ROS level in malignant cells that induced the intrinsic pathway of cell apoptosis. PAR-1 mediated apoptosis by HAP of malignant breast cells without effecting normal healthy cells in the same environment makes it a good therapeutic agent for treatment of cancer.

  17. Lipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 2.

    Science.gov (United States)

    Bondì, Maria Luisa; Montana, Giovanna; Craparo, Emanuela Fabiola; Di Gesù, Roberto; Giammona, Gaetano; Bonura, Angela; Colombo, Paolo

    2011-01-01

    Parietaria pollen is one of the major causes of allergic reaction in southern Europe, affecting about 30% of all allergic patients in this area. Specific immunotherapy is the only treatment able to modify the natural outcome of the disease by restoring a normal immunity against allergens. The preparation of allergen-solid lipid nanoparticles as delivery vehicles for therapeutic proteins, P. judaica major allergen Par j 2, was investigated. The Par j 2 allergen was expressed in a large amount in Escherichia coli and purified to homogeneity. Its immunological properties were studied by western blotting and enzyme-linked immunosorbent assay inhibition. Solid lipid nanoparticles were obtained by water-in-oil-in-water multiple emulsion method and characterized in terms of mean size and surface charge. These systems (approximately 250 nm diameter and negative surface charge) incorporated recombinant Par j 2 with 40% or greater efficiency. Moreover, the endotoxin level and anaphylactic activity of the empty solid lipid nanoparticles and recombinant Par j 2-loaded solid lipid nanoparticles were evaluated by looking at the overexpression of CD203c marker on human basophils. These results demonstrate that recombinant Par j 2-nanoparticles could be proposed as safe compositions for the development of new therapeutic dosage forms to cure allergic reactions.

  18. Valproate alters dopamine signaling in association with induction of Par-4 protein expression.

    Directory of Open Access Journals (Sweden)

    Saebom Lee

    Full Text Available Chromatin remodeling through histone modifications has emerged as a key mechanism in the pathophysiology of psychiatric disorders. Valproate (VPA, a first-line medication for bipolar disorder, is known to have histone deacetylase (HDAC inhibitor activity, but the relationship between its efficacy as a mood stabilizer and HDAC inhibitory activity is unclear. Here we provide evidence that prostate apoptosis response-4 (Par-4, an intracellular binding partner of dopamine D2 receptors (DRD2, plays a role in mediating the effectiveness of VPA. We found that chronic VPA treatment enhanced the expression of Par-4 in cultured neurons and adult mouse brains. This Par-4 induction phenomenon occurred at the transcriptional level and was correlated with an increase in histone H3 and H4 acetylation of the Par-4 promoter regions. Furthermore, chronic VPA treatment potentiated the suppression of the cAMP signaling cascade upon dopamine stimulation, which was blocked by sulpiride treatment. These results indicate that VPA potentiates DRD2 activity by enhancing Par-4 expression via a chromatin remodeling mechanism.

  19. Participatory action research (PAR) in middle school: opportunities, constraints, and key processes.

    Science.gov (United States)

    Ozer, Emily J; Ritterman, Miranda L; Wanis, Maggie G

    2010-09-01

    Late childhood and early adolescence represent a critical transition in the developmental and academic trajectory of youth, a time in which there is an upsurge in academic disengagement and psychopathology. PAR projects that can promote youth's sense of meaningful engagement in school and a sense of efficacy and mattering can be particularly powerful given the challenges of this developmental stage. In the present study, we draw on data from our own collaborative implementation of PAR projects in secondary schools to consider two central questions: (1) How do features of middle school settings and the developmental characteristics of the youth promote or inhibit the processes, outcomes, and sustainability of the PAR endeavor? and (2) How can the broad principles and concepts of PAR be effectively translated into specific intervention activities in schools, both within and outside of the classroom? In particular, we discuss a participatory research project conducted with 6th and 7th graders at an urban middle school as a means of highlighting the opportunities, constraints, and lessons learned in our efforts to contribute to the high-quality implementation and evaluation of PAR in diverse urban public schools.

  20. Lambeaux autofermants pour le traitement des brulures electriques du scalp par haut voltage

    Science.gov (United States)

    Hafidi, J.; El Mazouz, S.; El Mejatti, H.; Fejjal, N.; Gharib, N.E.; Abbassi, A.; Belmahi, A.M.

    2011-01-01

    Summary Les brûlures électriques par haut voltage sont responsables de gros dégâts tissulaires en immédiat et dans les jours suivant l’accident du fait de la chaleur importante dégagée par effet joule et de la thrombose microvasculaire évolutive. Les pertes de substances du scalp secondaires à ces brûlures nécessitent une couverture par lambeaux vu la destruction du périoste et du calvarium en regard. De juin 1997 à juin 2008, 15 patients ont été traités pour des pertes de substance du scalp secondaires à des brûlures électriques par haut voltage de diamètre allant de 8 à 11 cm et siégeant dans la région tonsurale. Ces patients ont été opérés dans la première semaine suivant l’accident. Les pertes de substance du scalp de taille moyenne secondaires à ces brûlures peuvent être couvertes per primam de façon fiable par des lambeaux locaux axialisés et multiples. Nous relatons l’expérience du Service de Chirurgie Plastique du Centre Hospitalier Universitaire Ibn-Sina, Rabat, Maroc, dans la gestion et la prise en charge de ces brûlures. PMID:22262963

  1. Regulation of mature T lymphocyte proliferation and differentiation by Par-4

    Science.gov (United States)

    Lafuente, María José; Martin, Pilar; Garcia-Cao, Isabel; Diaz-Meco, María Teresa; Serrano, Manuel; Moscat, Jorge

    2003-01-01

    The genetic inactivation of the atypical protein kinase C (aPKC) inhibitor, Par-4, gives rise to increased NF-κB activation and decreased stimulation of JNK in embryo fibroblasts. Here we have characterized the immunological phenotype of the Par-4–/– mice and found that the loss of this gene leads to an increased proliferative response of peripheral T cells when challenged through the TCR. This is accompanied by a higher increase in cell cycle entry and inhibition of apoptosis, with enhanced IL-2 secretion but normal CD25 synthesis. Interestingly, the TCR-triggered activation of NF-κB was augmented and that of JNK was severely abrogated. Consistent with previous data from knock outs of different JNKs, NFATc1 activation and IL-4 secretion were augmented in the Par-4-deficient CD4+ T cells, suggesting that the loss of Par-4 drives T-cell differentiation towards a Th2 response. This is compelling evidence that Par-4 is a novel modulator of the immune response through its ability to impact aPKC activity, which translates into lower JNK signaling. PMID:12970181

  2. Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology

    Science.gov (United States)

    Bache, Michael; Bosco, Filippo G.; Brøgger, Anna L.; Frøhling, Kasper B.; Sonne Alstrøm, Tommy; Hwu, En-Te; Chen, Ching-Hsiu; Eugen-Olsen, Jesper; Hwang, Ing-Shouh; Boisen, Anja

    2013-11-01

    In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody-antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable detection platform which can carry valuable prognostic information. In order to optimize the cantilever response the antibody immobilization and unspecific binding are initially characterized using quartz crystal microbalance technology. Also, the choice of antibody is explored in order to generate the largest surface stress on the cantilevers, thus increasing the signal. Using optimized experimental conditions the lowest detectable suPAR concentration is currently around 5 nM. The results reveal promising research strategies for the implementation of specific biochemical assays in a portable and high-throughput microsensor-based detection platform.

  3. MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.

    Directory of Open Access Journals (Sweden)

    Laura P Bernard

    Full Text Available The Par1 kinases, also known as microtubule affinity-regulating kinases (MARKs, are important for the establishment of cell polarity from worms to mammals. Dysregulation of these kinases has been implicated in autism, Alzheimer's disease and cancer. Despite their important function in health and disease, it has been unclear how the activity of MARK/Par1 is regulated by signals from cell surface receptors. Here we show that MARK/Par1 is activated downstream of NMDA receptors in primary hippocampal neurons. Further, we show that this activation is dependent on protein kinase A (PKA, through the phosphorylation of Ser431 of Par4/LKB1, the major upstream kinase of MARK/Par1. Together, our data reveal a novel mechanism by which MARK/Par1 is activated at the neuronal synapse.

  4. Protease-activated receptors (PARs) in cancer: Novel biased signaling and targets for therapy.

    Science.gov (United States)

    Bar-Shavit, R; Maoz, M; Kancharla, A; Jaber, M; Agranovich, D; Grisaru-Granovsky, S; Uziely, B

    2016-01-01

    Despite the fact that G protein-coupled receptors (GPCRs) mediate numerous physiological processes and represent targets for therapeutics for a vast array of diseases, their role in tumor biology is under appreciated. Protease-activated receptors (PARs) form a family which belongs to GPCR class A. PAR1&2 emerge with a central role in epithelial malignancies. Although the part of PAR1&2 in cancer is on the rise, their underlying signaling events are poorly understood. We review hereby past, present, and future cancer-associated PAR biology. Mainly, their role in physiological (placenta-cytotophobalst) and patho-physiological invasion processes. The identification and characterization of signal pleckstrin homology (PH)-domain-binding motifs established critical sites for breast cancer growth in PAR1&2. Among the proteins found to harbor important PH-domains and are involved in PAR biology are Akt/PKB as also Etk/Bmx and Vav3. A point mutation in PAR2, H349A, but not R352A, abrogated PH-protein association and is sufficient to markedly reduce PAR2-instigated breast tumor growth in vivo as also placental extravillous trophoblast (EVT) invasion in vitro is markedly reduced. Similarly, the PAR1 mutant hPar1-7A, which is unable to bind PH-domain, inhibits mammary tumors and EVT invasion, endowing these motifs with physiological significance and underscoring the importance of these previously unknown PAR1 and PAR2 PH-domain-binding motifs in both pathological and physiological invasion processes. Copyright © 2016 Elsevier Inc. All rights reserved.

  5. Causes de d?c?s des patients infect?s par le VIH dans le Centre tunisien

    OpenAIRE

    Chelli, Jih?ne; Bellazreg, Foued; Aouem, Abir; Hattab, Zouhour; Mesmia, H?la; Lasfar, Nadia Ben; Hachfi, Wissem; Masmoudi, Tasnim; Chakroun, Mohamed; Letaief, Amel

    2016-01-01

    La trith?rapie antir?trovirale a contribu? ? une baisse consid?rable de la mortalit? li?e au VIH. Les causes de d?c?s sont domin?es par les infections opportunistes dans les pays en voie de d?veloppement et par les maladies cardiovasculaires et les cancers dans les pays d?velopp?s. L?objectif ?tait de d?terminer les causes et les facteurs de risque de d?c?s des patients infect?s par le VIH dans le Centre Tunisien. Une ?tude transversale aupr?s des patients infect?s par le VIH ?g?s de plus de ...

  6. Transmission and Distribution of Photosymthetically Active Radiation (PAR) for Biomass Production in Exploration Missions [7216-050] Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Physical Sciences Inc. (PSI) proposes to develop a plant lighting system which collects, transmits and distributes photosynthetically active radiation (PAR) for...

  7. Transmission and Distribution of Photosynthetically Active Radiation (PAR) for Biomass Production in Exploration Missions [7226-270] Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Physical Sciences Inc. (PSI) proposes to develop a plant lighting system which collects, transmits and distributes photosynthetically active radiation (PAR) for...

  8. Endothelial progenitor cell-dependent angiogenesis requires localization of the full-length form of uPAR in caveolae.

    Science.gov (United States)

    Margheri, Francesca; Chillà, Anastasia; Laurenzana, Anna; Serratì, Simona; Mazzanti, Benedetta; Saccardi, Riccardo; Santosuosso, Michela; Danza, Giovanna; Sturli, Niccolò; Rosati, Fabiana; Magnelli, Lucia; Papucci, Laura; Calorini, Lido; Bianchini, Francesca; Del Rosso, Mario; Fibbi, Gabriella

    2011-09-29

    Endothelial urokinase-type plasminogen activator receptor (uPAR) is thought to provide a regulatory mechanism in angiogenesis. Here we studied the proangiogenic role of uPAR in endothelial colony-forming cells (ECFCs), a cell population identified in human umbilical blood that embodies all of the properties of an endothelial progenitor cell matched with a high proliferative rate. By using caveolae-disrupting agents and by caveolin-1 silencing, we have shown that the angiogenic properties of ECFCs depend on caveolae integrity and on the presence of full-length uPAR in such specialized membrane invaginations. Inhibition of uPAR expression by antisense oligonucleotides promoted caveolae disruption, suggesting that uPAR is an inducer of caveolae organization. Vascular endothelial growth factor (VEGF) promoted accumulation of uPAR in ECFC caveolae in its undegraded form. We also demonstrated that VEGF-dependent ERK phosphorylation required integrity of caveolae as well as caveolar uPAR expression. VEGF activity depends on inhibition of ECFC MMP12 production, which results in impairment of MMP12-dependent uPAR truncation. Further, MMP12 overexpression in ECFC inhibited vascularization in vitro and in vivo. Our data suggest that intratumor homing of ECFCs suitably engineered to overexpress MMP12 could have the chance to control uPAR-dependent activities required for tumor angiogenesis and malignant cells spreading.

  9. PAR3 and aPKC regulate Golgi organization through CLASP2 phosphorylation to generate cell polarity

    Science.gov (United States)

    Matsui, Toshinori; Watanabe, Takashi; Matsuzawa, Kenji; Kakeno, Mai; Okumura, Nobumasa; Sugiyama, Ikuko; Itoh, Norimichi; Kaibuchi, Kozo

    2015-01-01

    The organization of the Golgi apparatus is essential for cell polarization and its maintenance. The polarity regulator PAR complex (PAR3, PAR6, and aPKC) plays critical roles in several processes of cell polarization. However, how the PAR complex participates in regulating the organization of the Golgi remains largely unknown. Here we demonstrate the functional cross-talk of the PAR complex with CLASP2, which is a microtubule plus-end–tracking protein and is involved in organizing the Golgi ribbon. CLASP2 directly interacted with PAR3 and was phosphorylated by aPKC. In epithelial cells, knockdown of either PAR3 or aPKC induced the aberrant accumulation of CLASP2 at the trans-Golgi network (TGN) concomitantly with disruption of the Golgi ribbon organization. The expression of a CLASP2 mutant that inhibited the PAR3-CLASP2 interaction disrupted the organization of the Golgi ribbon. CLASP2 is known to localize to the TGN through its interaction with the TGN protein GCC185. This interaction was inhibited by the aPKC-mediated phosphorylation of CLASP2. Furthermore, the nonphosphorylatable mutant enhanced the colocalization of CLASP2 with GCC185, thereby perturbing the Golgi organization. On the basis of these observations, we propose that PAR3 and aPKC control the organization of the Golgi through CLASP2 phosphorylation. PMID:25518939

  10. Ataque de viento externo con manifestaciones de parálisis facial periférica tratado con acupuntura

    OpenAIRE

    Pinzón Ramírez, Betty Alejandra

    2012-01-01

    El ataque de viento externo es considerado desde la medicina china como un síndrome patológico que puede cursar con manifestaciones de parálisis facial semejante a la parálisis de Bell o parálisis facial idiopática de la medicina occidental, la cual también es efectivamente tratada con acupuntura; sin embargo en la actualidad no hay estudios al respecto en nuestra población. El objetivo es describir las características de población, presentación y evolución de la parálisis facial asociada ...

  11. Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR and concomitant activation of gelatinolytic enzymes.

    Directory of Open Access Journals (Sweden)

    Synnøve Magnussen

    Full Text Available The urokinase plasminogen activator receptor (uPAR is associated with poor prognosis in oral squamous cell carcinoma (OSCC, and increased expression of uPAR is often found at the invasive tumour front. The aim of the current study was to elucidate the role of uPAR in invasion and metastasis of OSCC, and the effects of various tumour microenvironments in these processes. Furthermore, we wanted to study whether the cells' expression level of uPAR affected the activity of gelatinolytic enzymes.The Plaur gene was both overexpressed and knocked-down in the murine OSCC cell line AT84. Tongue and skin tumours were established in syngeneic mice, and cells were also studied in an ex vivo leiomyoma invasion model. Soluble factors derived from leiomyoma tissue, as well as purified extracellular matrix (ECM proteins, were assessed for their ability to affect uPAR expression, glycosylation and cleavage. Activity of gelatinolytic enzymes in the tissues were assessed by in situ zymography.We found that increased levels of uPAR did not induce tumour invasion or metastasis. However, cells expressing low endogenous levels of uPAR in vitro up-regulated uPAR expression both in tongue, skin and leiomyoma tissue. Various ECM proteins had no effect on uPAR expression, while soluble factors originating from the leiomyoma tissue increased both the expression and glycosylation of uPAR, and possibly also affected the proteolytic processing of uPAR. Tumours with high levels of uPAR, as well as cells invading leiomyoma tissue with up-regulated uPAR expression, all displayed enhanced activity of gelatinolytic enzymes.Although high levels of uPAR are not sufficient to induce invasion and metastasis, the activity of gelatinolytic enzymes was increased. Furthermore, several tumour microenvironments have the capacity to induce up-regulation of uPAR expression, and soluble factors in the tumour microenvironment may have an important role in the regulation of posttranslational

  12. Helicophyllaceae (Bryophyta, nova ocorrência para o Estado do Pará, Brasil Helicophyllaceae (Bryophyta, a new record to Pará State, Brazil

    Directory of Open Access Journals (Sweden)

    Regina C. L. Lisboa

    2005-09-01

    Full Text Available Helicophyllaceae, família monotípica neotropical, é registrada pela primeira vez no Estado do Pará. Sua única espécie, Helicophyllum torquatum (Hook. Brid., registrada anteriormente no Estado do Amazonas e que recentemente havia sido excluída da lista de musgos amazônicos, é apresentada como nova ocorrência para toda a Amazônia brasileira.Helicophyllaceae, a monotypic family of Neotropics, is presented for the Pará State. Helicophyllum torquatum (Hook. Brid., the single species, was recorded for the Amazonas State, but recently was excluded of checklist of Amazonian mosses. H. torquatum is considered a new report for all Brazilian Amazônia.

  13. Potential Ecological Effects of Contaminants in the Exposed Par Pond Sediments

    Energy Technology Data Exchange (ETDEWEB)

    Paller, M.H. [Westinghouse Savannah River Company, AIKEN, SC (United States); Wike, L.D.

    1996-08-01

    Sediment and small mammal samples were collected from the exposed sediments of Par Pond in early 1995, shortly before the reservoir was refilled after a 4-year drawdown. Sampling was confined to elevations between 58 and 61 meters (190 and 200 feet) above mean sea level, which includes the sediments likely to be exposed if the Par Pond water level is permitted to fluctuate naturally. Both soil and small mammal samples were analyzed for a number of radionuclides and metals. Some of the soil samples were also analyzed for organic contaminants. The objective of the study was to determine if contaminant levels in the Par Pond sediments were high enough to cause deleterious ecological effects.

  14. Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells

    DEFF Research Database (Denmark)

    Hansen, Line; Unmack Larsen, Esben Kjær; Nielsen, Erik Holm

    2013-01-01

    patient prognosis shared by several cancers including breast, colorectal, and gastric cancers. Conjugation of a uPAR specific targeting peptide onto polyethylene glycol (PEG) coated USPIO nanoparticles by click chemistry resulted in a five times higher uptake in vitro in a uPAR positive cell line compared......Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted...... towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor...

  15. Development of a semi-parametric PAR partitioning model for the contiguous US

    Science.gov (United States)

    Kathilankal, J. C.; O'Halloran, T. L.; Schmidt, A.; Hanson, C. V.; Law, B. E.

    2014-03-01

    A semi-parametric PAR diffuse radiation model was developed using commonly measured climatic variables from 44 site-years of data from 9 AmeriFlux sites. The model has a logistic form and improves upon previous efforts, using a larger data set and physically viable climate variables as predictors, including relative humidity, clearness index, surface albedo, and solar elevation angle. Model performance was evaluated by comparison with a simple cubic polynomial model developed for the PAR spectral range. The logistic model outperformed the polynomial model with an improved coefficient of determination and slope relative to measured data (logistic: R2 = 0.85; slope = 0.86; cubic: R2 = 0.82; slope = 0.83), making this the most robust PAR-partitioning model for the US subcontinent currently available.

  16. Evaluation of Passive Autocatalytic Recombiner (PAR) Implementation in a Konvoi NPP Containment Type

    Energy Technology Data Exchange (ETDEWEB)

    Lopez-Alonso, E.; Papini, D.; Jimenez, G.

    2015-07-01

    The evaluation of Passive Autocatalytic Recombiner (PAR) implementation has been developed under the methodology extracted from the IAEA document, analysing the size, location and number of the PARs capable to minimize the combustion risk, which arises from a hydrogen release generated during a severe accident and its distribution in containment building. A detailed three-dimensional model of Konvoi (PWR) containment with GOTHIC 8.1 code was used for the simulations. The hydrogen preferential pathways and the accumulation points were studied and identified on the basis of a base case scenario without any mitigation measure. The PAR configuration offers an improvement in the chosen accidental scenario; decreasing the possibility of hydrogen combustion and leading to concentration values below the flammability limit (hydrogen concentration below 7%), in all the containment compartments at the end of the transient. Furthermore, from the analysis, it is concluded that the time required to reach hydrogen concentrations below the combustion limit is considerably reduced. (Author)

  17. The PARS sequence increase the efficiency of stable Pichia pastoris transformation.

    Science.gov (United States)

    Madsen, Claus Krogh; Vismans, Gilles; Brinch-Pedersen, Henrik

    2016-10-01

    The methylotrophic yeast Pichia pastoris is a popular host for recombinant expression of proteins. Plasmids containing the Pichia autonomously replicating sequence (PARS) transform P. pastoris with higher efficiency than linear DNA equipped with termini designed for homologous recombination. Moreover, PARS containing constructs provide higher protein yields. Unfortunately, these autonomous plasmids are inherently unstable and the preferred method of P. pastoris transformation is therefore stable integration in the genome by homologous recombination. In the present study we report that a novel combination of PARS and linearization of plasmids for P. pastoris transformation serves to significantly increase the transformation efficiency. Moreover, it is demonstrated that the constructs do not re-circularize but integrate stably into the P. pastoris genome. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Bufo & Spallanzani e a paródia pós-modernista

    Directory of Open Access Journals (Sweden)

    Saulo Brandão

    2016-02-01

    Full Text Available O presente trabalho tem por objetivo investigar as considerações acerca da paródia e sua implicação na linha teórica pós-modernista, partindo das análises de Fredric Jameson e Linda Hutcheon, quando conceituando pastiche. Desse modo, nos propomos a fazer uma análise das paródias no romance Bufo & Spallanzani, de Rubem Fonseca, a partir da leitura de Ana Cristina T. de B. Carvalho (2013 que corrobora para sonegar a proposta de Jameson. Desse modo, procuraremos, no emaranhado de citações do romance, que remetem aos mais diversos campos da cultura, elementos que qualifiquem a característica paródica do romance, tendo por embasamento a contraproposta feita por Linda Hutcheon.

  19. Lambeaux autofermants pour le traitement des brulures electriques du scalp par haut voltage

    OpenAIRE

    Hafidi, J.; El Mazouz, S.; El Mejatti, H.; Fejjal, N.; Gharib, N.E.; A. Abbassi; Belmahi, A.M.

    2011-01-01

    Les brûlures électriques par haut voltage sont responsables de gros dégâts tissulaires en immédiat et dans les jours suivant l’accident du fait de la chaleur importante dégagée par effet joule et de la thrombose microvasculaire évolutive. Les pertes de substances du scalp secondaires à ces brûlures nécessitent une couverture par lambeaux vu la destruction du périoste et du calvarium en regard. De juin 1997 à juin 2008, 15 patients ont été traités pour des pertes de substance du scalp secondai...

  20. Harmonization and development of resources and tools for Italian natural language processing within the PARLI project

    CERN Document Server

    Bosco, Cristina; Delmonte, Rodolfo; Moschitti, Alessandro; Simi, Maria

    2015-01-01

    The papers collected in this volume are selected as a sample of the progress in Natural Language Processing (NLP) performed within the Italian NLP community and especially attested by the PARLI project. PARLI (Portale per l’Accesso alle Risorse in Lingua Italiana) is a project partially funded by the Ministero Italiano per l’Università e la Ricerca (PRIN 2008) from 2008 to 2012 for monitoring and fostering the harmonic growth and coordination of the activities of Italian NLP. It was proposed by various teams of researchers working in Italian universities and research institutions. According to the spirit of the PARLI project, most of the resources and tools created within the project and here described are freely distributed and they did not terminate their life at the end of the project itself, hoping they could be a key factor in future development of computational linguistics.

  1. Toxina botulínica y fisioterapia en la parálisis cerebral infantil

    OpenAIRE

    Camilieri Rumbau, María Mercé

    2006-01-01

    Introducción: La parálisis cerebral es la causa más común de espasticidad en niños y la toxina botulínica A es un opción para su el tratamiento, ya que produce una parálisis local al ser inyectada en un músculo. Objetivo: Comprobar la eficacia de la aplicación de toxina botulínica y fisioterapia en la parálisis cerebral infantil. Material y método: Se ha realizado una búsqueda en diversas bases de datos electrónicas como son Freejournals, Doyma, Medline, Google y revistas de neuro...

  2. Paraplégie compliquant une plaie abdominale antérieure par arme blanche

    Science.gov (United States)

    Elahmadi, Brahim; Awab, Almahdi; El Moussaoui, Rachid; El Hijri, Ahmed; Azzouzi, Abderrahim; Alilou, Mustapha

    2015-01-01

    Les traumatismes médullaires sont des complications rares des plaies abdominales antérieures par arme blanche. Son diagnostic est difficile parfois retardé. L'imagerie par résonance magnétique reste l'examen de choix. Le traitement dépend du tableau clinique et de la gravité de la souffrance médullaire. Le pronostic est corrélé à l’étendue et à la nature de la lésion médullaire. Nous rapportons un cas exceptionnel d'un traumatisme médullaire chez une patiente victime d'une plaie abdominale antérieure par arme blanche. PMID:25995808

  3. Relación entre la Deseabilidad Social y los parámetros del Modelo Logístico de tres parámetros

    Directory of Open Access Journals (Sweden)

    Facundo Abal

    2015-05-01

    Full Text Available El objetivo de este trabajo es estudiar la relación de los parámetros a , b y c del modelo logístico de tres parámetros y la tendencia de los individuos a atribuirse a sí mismo cualidades socialmente deseables. Participaron 1592 personas que respondieron las escalas Perseverancia y Distorsión del Big Five Questionnaire (BFQ. Se aplicó el ML3p a los ítems de perseverancia mediante el programa MULTILOG. Previamente se debieron eliminar 3 reactivos para cumplir con la condición de unidimensionalidad requerida por el modelo. Como la escala Likert de los ítems de perseverancia tiene 5 de alternativas se contemplaron diferentes procedimientos para establecer 4 condiciones de dicotomización de los datos. Los parámetros de los ítems se estimaron el procedimiento de Máxima Verosimilitud Marginal a Posteriori. El ajuste del ML3p fue adecuado para los 9 ítems analizados. Considerando las diferentes condiciones de dicotomización se identificaron entre 3 y 5 ítems de cuya respuesta clave se asoció a mayores niveles de Distorsión. Sin embargo, los resultados obtenidos no permiten concluir que la respuesta influida por la deseabilidad social repercuta sobre alguno de los parámetros estudiados. Se discuten las limitaciones del presente estudio y se comentan otras líneas de investigación que pueden guiar a futuros estudios.

  4. Comparison of Direct Pars Repair Techniques of Spondylolysis in Pediatric and Adolescent Patients: Pars Compression Screw Versus Pedicle Screw-Rod-Hook.

    Science.gov (United States)

    Karatas, Ali F; Dede, Ozgur; Atanda, Alfred A; Holmes, Larry; Rogers, Kenneth; Gabos, Peter; Shah, Suken A

    2016-08-01

    Retrospective clinical cohort study. To compare the clinical and radiographic outcomes of patients who were treated with intrasegmental pars fixation by either laminar compression screw (LS) or a pedicle screw, rod, and laminar hook (PSRH) construct. Spondylolysis is a nonunion defect of the pars interarticularis. In symptomatic spondylolysis, direct repair of the pars interarticularis defect can preserve motion and prevent abnormal stresses at the adjacent levels. Sixteen patients who failed nonoperative treatment and underwent direct pars repair by using LS (n=9) or PSRH (n=7) constructs were included in the study. Clinical outcome was assessed by using the MacNab criteria. Radiologic fusion and complications were evaluated using plain radiographs or computed tomography images and patient charts. The healing rate was 100% after 6 months. The healing time was similar in both the groups: LS, 6.5 months; PSRH, 6.2 months. Patients with PSRH (5.9 mo) were more likely to return to sports earlier relative to patients with LS (7.7 mo). There were no complications in the LS group; in the PSRH group, 1 patient had mild sensory deficit and 2 had superficial wound infections. The MacNab criteria for pain assessment showed an excellent or good outcome in 8 of 9 patients in LS group and 6 of 7 patients in PSRH group. Relative to LS patients, there was a significant increase in surgical time and estimated blood loss among PSRH patients. Either of the mentioned 2 techniques appears to produce acceptable results. Biplanar fluoroscopy and navigation systems could minimize the risk of screw misplacement with LS construct. Familiarity with the various fixation techniques will allow the surgeon to select the most appropriate surgical technique.

  5. Expression of JAM-A, AF-6, PAR-3 and PAR-6 during the assembly and remodeling of RPE tight junctions.

    Science.gov (United States)

    Luo, Yan; Fukuhara, Masayuki; Weitzman, Matthew; Rizzolo, Lawrence J

    2006-09-19

    The tight junctions of the endothelial and epithelial regions of the blood-brain barrier are regulated by interactions with the neighboring tissue. We examined how the neural retina regulates the assembly of tight junctions in the retinal pigment epithelium (RPE). The proteins JAM-A, AF-6, PAR-3 and PAR-6 have been implicated in the assembly of other epithelial tight junctions. Using chick embryos and primary cell culture, we examined gene expression of these proteins during embryonic development, and whether retinal secretions regulate their expression. Three highly conserved RNA splice sites of AF-6 were identified in chick ocular tissues, but only two were expressed in RPE. JAM-A and AF-6 were expressed at relatively high levels early in development when adherens junctions form, but before tight junctions form. Expression of JAM-A and the AF-6 isoforms actually decreased when tight junctions were forming and expanding. The expression of PAR-3 and PAR-6 was constant. Despite the expression of these proteins in vitro (along with claudins, occludin, ZO-1 and ZO-2), the tight junctional networks that form were discontinuous (Rahner, C., Fukuhara, M., Peng, S., Kojima, S., Rizzolo, L.J., 2004. The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development. J. Cell Sci. 117, 3307-3318). The expression of these assembly proteins was unaffected by a retinal conditioned medium that induced the completion of tight junction formation. These data indicate that the early expression of the assembly proteins corresponds to the initial establishment of the adherens and tight junctions, but secretory products of the neural retina must induce the expression of additional proteins to complete the maturation process.

  6. Analysis of some sepsis markers: C-reactive protein, Procalcitonin, Osteopontin and suPAR

    Directory of Open Access Journals (Sweden)

    Ilaria Crespi

    2010-09-01

    Full Text Available Sepsis appears to be due to an uncontrolled pro and anti-inflammatory response leading to organ dysfunction until shock and death.Accuracy of diagnosis and appropriate treatment affect the outcome. Aim of the study was to investigate if the levels of Osteopontin (OPN and Soluble Urokinase-type Plasminoge Activator Receptor (suPAR might be early markers of sepsis and if these markers play a role in predicting the progression to septic shock. Methods:The levels of OPN, suPAR, C-reactive protein (CRP and procalcitonin (PCT were measured in patients at higher risk of infection. The samples were collected from the day of admission for the following fifteenth day. During recovery Sistemic Inflammatory Response Syndrome (SIRS criteria, blood chemistry data, blood gas- analysis, lactate and diuresis were collected. Microbiological culture were performed according to the clinical condition. Results: 29 patients were enrolled in the study, including 14 subjects with positive blood cultures.The values of OPN, PCT, suPAR and CRP were significantly increased in patients with positive blood cultures than those with negative blood cultures.Analysing changes of the levels of OPN, suPAR, PCT and CRP in patients with septic shock, the values of OPN increased in advance to the clinical diagnosis of septic shock, while the values of suPAR and PCT slight delay compared to those OPN.The levels of CRP were independent from the onset of septic shock state. Conclusions: These preliminary data suggest that Osteopontin and suPAR can be used as early markers of sepsis and might be useful in monitoring of septic outcome to predict a possible evolution to septic shock.

  7. Characterization of a dominant antigenic determinant of Par o I encoded by recombinant DNA.

    Science.gov (United States)

    Scotto d'Abusco, A S; De Santo, C; Menna, T; Coscia, M R; Oreste, U; Geller-Bernstein, C; Ruffilli, A

    1996-02-01

    The pollens from Parietaria judaica and Parietaria officinalis are a major cause of pollinosis in Europe. Par o I (13.5 kDa) and Par j I (12 kDa), the major allergens from these species, are highly crossreactive. We have immunoscreened a P. judaica pollen cDNA expression library with a rabbit antiserum specific for Par j I and with a serum pool from allergic patients. An immunopositive clone containing a 26 bp insert was further characterized. The insert sequence was determined and the beta-galactosidase fusion protein was partially purified by electroelution from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. This fusion protein specifically and extensively inhibited Par o I and Par j I binding of a rabbit antiserum and of a serum pool obtained from allergic patients. The antifusion-protein antiserum obtained in a rabbit (anti 6a) specifically precipitated radioiodinated purified Par o I in the double antibody radioimmunoassay (DARIA) and competed with antibodies of sera from allergic patients for the binding to Parietaria pollen extract allergens by enzyme linked immunosorbent assay (ELISA). We investigated the prevalence of antibody response towards the 6a epitope in patients naturally sensitized to Parietaria. The presence of 6a specific IgE antibodies was assessed in the sera of 33 patients using inhibition assays. All sera had antibodies with this specificity: the extensive percentage of inhibition reached suggested that they dominated individual ab response. In conclusion, the antibody response induced by natural exposure to the pollen of Parietaria appears to be higly focused on a single linear antigenic determinant of the major allergens which may play a relevant role in the development of clinical allergy. This report is, to our knowledge, the first description of a dominant linear epitope of a major allergen.

  8. Linking the fPAR, forest albedo and biomass in the northern biomes of Europe

    Science.gov (United States)

    Lukeš, Petr; Stenberg, Pauline; Manninen, Terhikki; Rautiainen, Miina; Mõttus, Matti

    2014-05-01

    Land surface albedo and the fraction of photosynthetically active radiation (fPAR) absorbed by plant canopies are two of the essential climate variables controlling the planetary radiative energy budget. Albedo is directly related to the energy exchange between land and the atmosphere as it is the reflectivity of the surface - the higher the albedo, the more incoming solar radiation is reflected and the less absorbed by the surface. The fPAR is related to plant productivity, quantifying the amount of absorbed light available for photosynthesis. It is a key parameter in the modelling of net primary production (NPP) of terrestrial ecosystems. Global climate scenarios are very sensitive to albedo and fPAR estimates, and thus, the effect of changes in canopy structure and density (biomass) on these two variables needs to be quantified reliably. Both parameters are routinely retrieved from current Earth Observation sensors using specialized algorithms. To date, these satellite products have not been linked to extensive forest inventory data sets due to the lack of ground reference data. Data availability for Finland has significantly improved in December 2012, when National Forest Inventory (NFI) data became freely available to the public. The dataset covers the geographical area of Finland (26.1 million hectares) at a spatial resolution of 20 meters including several forest structural variables. In this study, we use the NFI data to study the links between forest albedo, fPAR and forest structure and density during the green vegetation season. More specifically, we investigated the seasonal trends in fPAR and albedo of different spectral regions of northern forests. Empirical relationships between forest albedo, fPAR and total aboveground biomass were established for selected days within the vegetation growing period and across a latitudinal transect of Finland.

  9. Protease activated receptors (PARS) mediation in gyroxin biological activity; Mediacao dos receptores ativados por proteases (PARs) em atividades biologicas da giroxina

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Jose Alberto Alves da

    2009-07-01

    Gyroxin is a serine protease enzyme from the South American rattlesnake (Crotalus durissus terrificus) venom; it is only partially characterized and has multiple activities. Gyroxin induces blood coagulation, blood pressure decrease and a neurotoxic behavior named barrel rotation. The mechanisms involved in this neurotoxic activity are not known. Whereas gyroxin is a member of enzymes with high potential to become a new drug with clinical applications such as thrombin, batroxobin, ancrod, tripsyn and kalicrein, it is important to find out how gyroxin works. The analysis on agarose gel electrophoresis and circular dichroism confirmed the molecules' integrity and purity. The gyroxin intravenous administration in mice proved its neurotoxicity (barrel rotation). In vivo studies employing intravital microscopy proved that gyroxin induces vasodilation with the participation of protease activated receptors (PARs), nitric oxide and Na+K+ATPase. The leukocytes' adherence and rolling counting indicated that gyroxin has no pro inflammatory activity. Gyroxin induced platelet aggregation, which was blocked by inhibitors of PAR1 and PAR4 receptors (SCH 79797 and tcY-NH{sub 2}, respectively). Finally, it was proved that the gyroxin temporarily alter the permeability of the blood brain barrier (BBB). Our study has shown that both the protease-activated receptors and nitric oxide are mediators involved in the biological activities of gyroxin. (author)

  10. Identification of marker genes for pars tuberalis morphogenesis in chick embryo: expression of Cytokine-like 1 and Gap junction protein alpha 5 in pars tuberalis.

    Science.gov (United States)

    Aizawa, Sayaka; Higaki, Yuriko; Dudaui, Amrita; Nagasaka, Mai; Takahashi, Sumio; Sakata, Ichiro; Sakai, Takafumi

    2016-12-01

    The adenohypophysis is formed from the oral ectoderm and consists of the pars distalis (PD), pars intermedia, and pars tuberalis (PT). The mechanisms of PD development have been extensively studied, and the cellular differentiation of the PD is well understood. However, the morphogenesis and differentiation of the PT are still unclear, and the genes expressed during PT development remain largely unknown. We have explored genes specifically expressed in the PT during embryonic development and analyzed their spatiotemporal expression patterns. Microarray analysis of laser-captured PT and PD tissues obtained from chick embryos on embryonic day 10 (E10.0) has shown high expression of Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) genes in the PT. Detailed analysis of these spatiotemporal expression patterns during chick embryo development by in situ hybridization has revealed that CYTL1 mRNA first appears in the lateral head ectoderm and ventral head ectoderm at E1.5. The expression of CYTL1 moves into Rathke's pouch at E2.5 and is then localized in the PT primordium where it is continuously expressed until E12.0. GJA5 mRNA is transiently detected in the PT primordium from E6.0 to E12.0, whereas its expression is not detected in the PD during development. Thus, these genes might be involved in the regulation mechanisms of PT development and could be useful markers for PT development.

  11. Parásitos de Fasciola hepática intracoledociano

    OpenAIRE

    MILLÁN A,MARCELO; WAGENKNECHT S,RAUL; CARDENAS P,ALEX; CARRASCO L,CRISTIAN

    2008-01-01

    La fasciolasis es una enfermedad parasitaria producida por el tremátodo digeneo Fasciola hepática. Durante la fase crónica de la enfermedad los parásitos pueden entran en los conductos biliares, y ser causa de ictericia obstructiva, colangitis, pancreatitis y otras complicaciones. Se presenta el caso clínico de una paciente intervenida por sospecha de colelitiasis y colédocolitiasis, a la cual durante la exploración coledociana intraoperatoria le fueron extraídos múltiples parásitos adultos d...

  12. Miasis oral en un paciente con parálisis cerebral: Reporte de un caso

    OpenAIRE

    Hernán Arango Fernández; Martha Rebolledo Cobos; Marisol Sánchez Molina; Pablo Roa Rojas; Pedro Felipe Méndez Malagón

    2016-01-01

    La Miasis Oral es una infección parasitaria ocasionada por larvas de mosca que pueden afectar tejido vivo o necrótico, etiológicamente se asocia a deficiente higiene oral, lesiones supurativas, alcoholismo, pacientes seniles, parálisis cerebral, entre otras condiciones que predisponen al depósito de estas larvas. El presente caso evidencia una paciente femenina de 22 años de edad con diagnóstico de parálisis cerebral y desnutrición severa, la cual es llevada por acudiente presentando tumefacc...

  13. Calidad y parálisis del sueño en estudiantes de medicina

    OpenAIRE

    Charles Huamaní; Arturo Reyes; Percy Mayta-Tristán; Raúl Timana; Abel Salazar; David Sánchez; Hugo Pérez

    2006-01-01

    Introducción: La parálisis del sueño es un evento de inmovilización corporal estando consciente, ocurre espontáneamente o secundaria a situaciones estresantes o una posible mala calidad de sueño. Objetivo: Determinar la asociación y riesgo de tener parálisis del sueño en los estudiantes de la facultad de medicina, que son malos dormidores. Diseño: Estudio analítico, transversal. Lugar: Facultad de Medicina de la Universidad Nacional Mayor de San Marcos. Participantes: Estudiantes de medicina....

  14. Calidad y parálisis del sueño en estudiantes de medicina

    OpenAIRE

    Huamaní, Charles; Universidad Nacional Mayor de San Marcos. Lima, Perú; Sociedad Científica de San Fernando. Lima, Perú; Sociedad Científica Médico Estudiantil Peruana. Lima, Perú; Reyes, Arturo; Instituto Especializado en Ciencias Neurológicas Oscar Trelles Montes. Lima, Perú; Mayta-Tristán, Percy; Instituto Nacional de Salud. Lima, Perú; Timana, Raúl; Universidad Nacional Mayor de San Marcos. Lima, Perú; Sociedad Científica de San Fernando. Lima, Perú; Salazar, Abel; Universidad Nacional Mayor de San Marcos. Lima, Perú; Sociedad Científica de San Fernando. Lima, Perú; Sánchez, David; Universidad Nacional Mayor de San Marcos. Lima, Perú; Sociedad Científica de San Fernando. Lima, Perú; Pérez, Hugo; Universidad Nacional Mayor de San Marcos. Lima, Perú; Sociedad Científica de San Fernando. Lima, Perú

    2013-01-01

    Introducción: La parálisis del sueño es un evento de inmovilización corporal estando consciente, ocurre espontáneamente o secundaria a situaciones estresantes o una posible mala calidad de sueño. Objetivo: Determinar la asociación y riesgo de tener parálisis del sueño en los estudiantes de la facultad de medicina, que son malos dormidores. Diseño: Estudio analítico, transversal. Lugar: Facultad de Medicina de la Universidad Nacional Mayor de San Marcos. Participantes: Estudiantes de medicina....

  15. Fait marquant : discussions sur les données ouvertes entamées par ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    14 avr. 2016 ... La santé dans les Caraïbes Contrer l'obésité par la diversité des aliments locaux. Dans les Caraïbes, la conjugaison de l'augmentation des importations d'aliments transformés et de la faible consommation d'aliments sains comme les. Voir davantageLa santé dans les Caraïbes Contrer l'obésité par la ...

  16. Le Sycoscan : un système d'inspection des conteneurs par balayage radioscopique

    CERN Multimedia

    Krystal Production

    1997-01-01

    La société Europ Scan, le port autonome du Havre et les douanes françaises ont conçu un système de contrôle par rayons X qui permet d’étudier par radiographie le contenu d'un chargement. Les conteneurs sont ainsi vérifiés sans être déchargés ou ouverts. On utilise des faisceaux de rayons X de faible énergie couplés à des détecteurs ultrasensibles inspirés de la chambre à fils de Georges Charpak.

  17. Les plantes des galeries forestières à usage traditionnel par les ...

    African Journals Online (AJOL)

    Des enquêtes ethnobotaniques ont été réalisées de 2006 à 2010 dans les provinces de la Comoé et du Kénédougou localisées dans l'Ouest du Burkina Faso. Ces provinces sont majoritairement peuplées par l'ethnie Sénoufo. Elles sont caractérisées par un climat relativement humide, une présence de rivières ...

  18. Freehand technique for C2 pedicle and pars screw placement: safe or not?

    Science.gov (United States)

    Punyarat, Prachya; Riew, K Daniel; Klawson, Benjamin T; Peters, Colleen; Lertudomphonwanit, Thamrong; Buchowski, Jacob M

    2017-11-16

    During placement of C2 pedicle and pars screws, intraoperative fluoroscopy is used so that neurovascular complications can be avoided, and screws can be placed in the proper position. However, this method is time consuming and increases radiation exposure. Furthermore, it does not guarantee completely safe and accurate screw placement. To evaluate the safety of the C2 pedicle and pars screw placement without fluoroscopic or other guidance methods. Retrospective comparative study. One hundred and ninety-eight patients who underwent placement of C2 pedicle or pars screws without any intraoperative radiographic guidance were included. Medical records and postoperative CT scans were evaluated. Clinical data were reviewed for intraoperative and postoperative complications. Accuracy of screw placement was evaluated with post-op CT scans using a previously published cortical-breach grading system (described by location and percentage of screw diameter over cortical edge (0 = none; grade I = pars screws were inserted by two experienced surgeons. There were no cases of CSF leakage and no neurovascular complications during screw placement. Postoperative CT scans were available for 76 patients, which included 52 pedicle and 87 pars screws. For cases with C2 pedicle screws, there were 12 breaches (23%); these included 10 screws with a grade I breach (19%), 1 screw with a grade II breach (2%), and 1 screw with a grade IV breach (2%). Lateral breaches occurred in 7 screws (13%), inferior breaches in 3 (6%), and superior breaches in 2 (4%). For cases with C2 pars screws, there were 10 breaches (11%); these included 6 screws with a grade I breach (7%), 2 screws with a grade II breach (2%), and 2 screws with a grade IV breach (2%). Medial breaches were found in 4 (5%), lateral breaches in 2 (2%), inferior breaches in 2 (2%), and superior breaches in 2 (2%). 2 of the cases with superior breaches (1 for pedicle and 1 for pars) experienced occipital neuralgia months after surgery

  19. Modeles numeriques de la stimulation optique de neurones assistee par nanoparticules plasmoniques

    Science.gov (United States)

    Le Hir, Nicolas

    La stimulation de neurones par laser emerge depuis plusieurs annees comme une alternative aux techniques plus traditionnelles de stimulation artificielle. Contrairement a celles-ci, la stimulation lumineuse ne necessite pas d'interagir directement avec le tissu organique, comme c'est le cas pour une stimulation par electrodes, et ne necessite pas de manipulation genetique comme c'est le cas pour les methodes optogenetiques. Plus recemment, la stimulation lumineuse de neurones assistee par nanoparticules a emerge comme un complement a la stimulation simplement lumineuse. L'utilisation de nanoparticules complementaires permet d'augmenter la precision spatiale du procede et de diminuer la fluence necessaire pour observer le phenomene. Ceci vient des proprietes d'interaction entre les nanoparticules et le faisceau laser, comme par exemple les proprietes d'absorption des nanoparticules. Deux phenomenes princpaux sont observes. Dans certains cas, il s'agit d'une depolarisation de la membrane, ou d'un potentiel d'action. Dans d'autres experiences, un influx de calcium vers l'interieur du neurone est detecte par une augmentation de la fluorescence d'une proteine sensible a la concentration calcique. Certaines stimulations sont globales, c'est a dire qu'une perturbation se propage a l'ensemble du neurone : c'est le cas d'un potentiel d'action. D'autres sont, au contraire, locales et ne se propagent pas a l'ensemble de la cellule. Si une stimulation lumineuse globale est rendue possible par des techniques relativement bien maitrisees a l'heure actuelle, comme l'optogenetique, une stimulation uniquement locale est plus difficile a realiser. Or, il semblerait que les methodes de stimulation lumineuse assistees par nanoparticules puissent, dans certaines conditions, offrir cette possibilite. Cela serait d'une grande aide pour conduire de nouvelles etudes sur le fonctionnement des neurones, en offrant de nouvelles possibilites experimentales en complement des possibilites

  20. Tratamiento exitoso de un hemangioma de parótida con propranolol

    OpenAIRE

    Edelisa Moredo Romo; Fernanda Pastrana Fundora

    2012-01-01

    Los hemangiomas infantiles son los tumores vasculares benignos más frecuentes en la infancia, que ocurren mayormente en los pacientes pretérmino, en un 80 % afectan cabeza y cuello, y en algunos pacientes provocan serias complicaciones para la vida. El hemangioma de parótida es una forma clínica de presentación en la que suele verse un período proliferativo prolongado con lenta involución. Se presenta el caso clínico de un paciente con diagnóstico de hemangioma de parótida con componente supe...

  1. F-actin-like filaments formed by plasmid segregation protein ParM

    DEFF Research Database (Denmark)

    van den Ent, Fusinita; Møller-Jensen, Jakob; Amos, Linda A.

    2002-01-01

    It was the general belief that DNA partitioning in prokaryotes is independent of a cytoskeletal structure, which in eukaryotic cells is indispensable for DNA segregation. Recently, however, immunofluorescence microscopy revealed highly dynamic, filamentous structures along the longitudinal axis...... of Escherichia coli formed by ParM, a plasmid-encoded protein required for accurate segregation of low-copy-number plasmid R1. We show here that ParM polymerizes into double helical protofilaments with a longitudinal repeat similar to filamentous actin (F-actin) and MreB filaments that maintain the cell shape...

  2. Prise en charge des péritonites par perforation d'ulcères gastro ...

    African Journals Online (AJOL)

    Le but de cette étude a été de décrire la prise en charge des péritonites aiguës généralisées par perforation d'ulcère gastrique ou duodénal et d'évaluer le bénéfice du drainage abdominal. Nous avons réalisé une étude transversale sur 3 ans. Elle a inclus 129 patients opérés pour péritonites par perforation gastrique ou ...

  3. PET imaging of urokinase-type plasminogen activator receptor (uPAR) in prostate cancer

    DEFF Research Database (Denmark)

    Skovgaard, Dorthe; Persson, Morten; Kjaer, Andreas

    2016-01-01

    (intact/cleaved forms)—provides independent additional clinical information to that contributed by PSA, Gleason score, and other relevant pathological and clinical parameters. In this respect, non-invasive molecular imaging by positron emission tomography (PET) offers a very attractive technology platform......, which can provide the required quantitative information on the uPAR expression profile, without the need for invasive procedures and the risk of missing the target due to tumor heterogeneity. These observations support non-invasive PET imaging of uPAR in PC as a clinically relevant diagnostic...

  4. La synergie entre le féminisme et la gauche québécoise vu à travers quelques débats parus dans La Vie En Rose et Le Temps Fou

    National Research Council Canada - National Science Library

    Jacinthe Michaud

    2016-01-01

    ... la première manifestation en 1969. [Les] années 70 porte la marque d'un féminisme marqué par un petit nombre de groupes autonomes de femmes qui n'avaientd'autres choixquede travailler ensemblepourproduireuneplateforme politique cohérente. Du côté...

  5. Films minces de dioxyde de titane déposés sur titane par mocvd : microstructure et biocomptabilité

    OpenAIRE

    Jacota Popescu, Simona Andreia

    2008-01-01

    Des films de TiO2 variant par le teneur relative en anatase et en rutile, par la rugosité, par la mouillabilité, par la morphologie de section transverse ont été déposés sur des plaquettes de titane par MOCVD à basse pression. Du titane oxydé par anodisation ou par explosion à l'air a aussi été préparé. La capacité des dépôts à protéger le titane contre la corrosion par un fluide biologique simulé s'est avérée supérieure à celle de l'oxyde naturel. Le comportement des fibroblastes et d'ostéob...

  6. PR3 and elastase alter PAR1 signaling and trigger vWF release via a calcium-independent mechanism from glomerular endothelial cells.

    Science.gov (United States)

    Tull, Samantha P; Bevins, Anne; Kuravi, Sahithi Jyothsna; Satchell, Simon C; Al-Ani, Bahjat; Young, Stephen P; Harper, Lorraine; Williams, Julie M; Rainger, George Ed; Savage, Caroline O S

    2012-01-01

    Neutrophil proteases, proteinase-3 (PR3) and elastase play key roles in glomerular endothelial cell (GEC) injury during glomerulonephritis. Endothelial protease-activated receptors (PARs) are potential serine protease targets in glomerulonephritis. We investigated whether PAR1/2 are required for alterations in GEC phenotype that are mediated by PR3 or elastase during active glomerulonephritis. Endothelial PARs were assessed by flow cytometry. Thrombin, trypsin and agonist peptides for PAR1 and PAR2, TFLLR-NH(2) and SLIGKV-NH(2,) respectively, were used to assess alterations in PAR activation induced by PR3 or elastase. Endothelial von Willebrand Factor (vWF)release and calcium signaling were used as PAR activation markers. Both PR3 and elastase induced endothelial vWF release, with elastase inducing the highest response. PAR1 peptide induced GEC vWF release to the same extent as PR3. However, knockdown of PARs by small interfering RNA showed that neither PAR1 nor PAR2 activation caused PR3 or elastase-mediated vWF release. Both proteases interacted with and disarmed surface GEC PAR1, but there was no detectable interaction with cellular PAR2. Neither protease induced a calcium response in GEC. Therefore, PAR signaling and serine protease-induced alterations in endothelial function modulate glomerular inflammation via parallel but independent pathways.

  7. PR3 and elastase alter PAR1 signaling and trigger vWF release via a calcium-independent mechanism from glomerular endothelial cells.

    Directory of Open Access Journals (Sweden)

    Samantha P Tull

    Full Text Available Neutrophil proteases, proteinase-3 (PR3 and elastase play key roles in glomerular endothelial cell (GEC injury during glomerulonephritis. Endothelial protease-activated receptors (PARs are potential serine protease targets in glomerulonephritis. We investigated whether PAR1/2 are required for alterations in GEC phenotype that are mediated by PR3 or elastase during active glomerulonephritis. Endothelial PARs were assessed by flow cytometry. Thrombin, trypsin and agonist peptides for PAR1 and PAR2, TFLLR-NH(2 and SLIGKV-NH(2, respectively, were used to assess alterations in PAR activation induced by PR3 or elastase. Endothelial von Willebrand Factor (vWFrelease and calcium signaling were used as PAR activation markers. Both PR3 and elastase induced endothelial vWF release, with elastase inducing the highest response. PAR1 peptide induced GEC vWF release to the same extent as PR3. However, knockdown of PARs by small interfering RNA showed that neither PAR1 nor PAR2 activation caused PR3 or elastase-mediated vWF release. Both proteases interacted with and disarmed surface GEC PAR1, but there was no detectable interaction with cellular PAR2. Neither protease induced a calcium response in GEC. Therefore, PAR signaling and serine protease-induced alterations in endothelial function modulate glomerular inflammation via parallel but independent pathways.

  8. Equações de chuvas intensas para o estado do Pará Intense rainfall equations for the state of Pará, Brazil

    Directory of Open Access Journals (Sweden)

    Rodrigo O. R. de M. Souza

    2012-09-01

    Full Text Available As equações de chuvas intensas têm sido usadas como ferramenta importante para o dimensionamento de obras hidráulicas. Devido à grande carência de informações relativas às equações de chuvas intensas, o presente trabalho teve como objetivo a obtenção das relações de intensidade, duração e frequência de precipitação pluvial para o Estado do Pará, utilizando-se a metodologia da desagregação da chuva de 24 h. Foram utilizadas séries históricas de dados pluviométricos de 74 cidades do Estado do Pará, obtidas no Sistema de Informações Hidrológicas da Agência Nacional de Águas-ANA. As equações de intensidade-duração-frequência foram devidamente ajustadas e apresentaram bom ajuste, com coeficientes de determinação acima de 0,99. A maioria das estações (51,4% apresentou intensidade de precipitação entre 90 e 110 mm h-1, para uma duração de chuva de 30 min e um tempo de retorno de 15 anos. Pode-se perceber uma concentração das maiores precipitações na região próxima ao litoral do nordeste paraense e no sudeste da Ilha do Marajó.The intense rainfall equations have been used as an important tool for design of hydraulic structures. Considering the lack of intense rainfall equations, this study aimed to determine the relations of intensity, duration and frequency of intense rainfall in the Pará State (Brazil, using the one-day rain disaggregation method. In this research rainfall data of 74 cities in the State of Pará were used, obtained from the Hydrological Information System of the National Water Agency-ANA. The equations of intensity-duration-frequency were adjusted and presented good adjustment with coefficients of determination above 0.99. Most stations (51.4% showed intensity of precipitation between 90 and 110 mm h-1 for duration of 30 min and rainfall return period of 15 years. The highest rainfall intensities were in the region near the northeast coast of Pará State and southeast of the Marajo

  9. écrivain-e. Par-delà le genre – par-delà le corps ?

    Directory of Open Access Journals (Sweden)

    Cécile Chamayou-Kuhn

    2009-12-01

    Full Text Available Cet article propose d’étudier la relation qu’entretiennent, d’une part, le langage et le corps et, d’autre part, la parole et l’identité sexuelle dans Die Kränkung d’Evelyn Schlag et Der Tod und das Mädchen : III (Rosamunde ; V (Die Wand d’Elfriede Jelinek. Malgré les divergences de ton que présentent ces oeuvres, qui appartiennent à deux genres littéraires différents, il semble permis de centrer l’analyse sur une problématique commune : le personnage de « l’écrivain-e » parvient-il à saisir son propre corps ? Dans cette perspective, nous nous appuyons sur la critique formulée par Judith Butler à l’égard de la psychanalyse lacanienne, tout en considérant sa théorie sur la construction discursive du corps. Trois thématiques peuvent être développées : la dialectique de « l’in-visibilité » féminine, les processus de matérialisation du corps et la pratique subversive de la mascarade en tant qu’elle met en évidence certains mécanismes d’aliénation dans le cadre d’une genèse du corps et de l’écriture. Aussi peut-on affirmer que le drame du corps investi symboliquement sous-tend ces œuvres : l’écrivain-e tente de s’extraire de l’ordre symbolique et de saper les fondements des catégories constitutives de l’identité que sont le désir, le sexe biologique (sex et l’identité sexuelle (gender. Si les processus d’écriture laissent envisager un dépassement de l’offense narcissique et de la maladie dans Die Kränkung, un tel dénouement paraît exclu dans l’univers jelinekien : ses « héroïnes », qui évoluent comme autant de fantômes du signifiant, entament une éternelle danse de mort.Untersucht wird im folgenden Artikel – anhand der Texte Die Kränkung von Evelyn Schlag und Der Tod und das Mädchen III (Rosamunde und V (Die Wand von Elfriede Jelinek – das Verhältnis von Sprache zum Körper sowie die Beziehung zwischen Sprechen und

  10. Planar Cell Polarity Breaks the Symmetry of PAR Protein Distribution prior to Mitosis in Drosophila Sensory Organ Precursor Cells.

    Science.gov (United States)

    Besson, Charlotte; Bernard, Fred; Corson, Francis; Rouault, Hervé; Reynaud, Elodie; Keder, Alyona; Mazouni, Khalil; Schweisguth, François

    2015-04-20

    During development, cell-fate diversity can result from the unequal segregation of fate determinants at mitosis. Polarization of the mother cell is essential for asymmetric cell division (ACD). It often involves the formation of a cortical domain containing the PAR complex proteins Par3, Par6, and atypical protein kinase C (aPKC). In the fly notum, sensory organ precursor cells (SOPs) divide asymmetrically within the plane of the epithelium and along the body axis to generate two distinct cells. Fate asymmetry depends on the asymmetric localization of the PAR complex. In the absence of planar cell polarity (PCP), SOPs divide with a random planar orientation but still asymmetrically, showing that PCP is dispensable for PAR asymmetry at mitosis. To study when and how the PAR complex localizes asymmetrically, we have used a quantitative imaging approach to measure the planar polarization of the proteins Bazooka (Baz, fly Par3), Par6, and aPKC in living pupae. By using imaging of functional GFP-tagged proteins with image processing and computational modeling, we find that Baz, Par6, and aPKC become planar polarized prior to mitosis in a manner independent of the AuroraA kinase and that PCP is required for the planar polarization of Baz, Par6, and aPKC during interphase. This indicates that a "mitosis rescue" mechanism establishes asymmetry at mitosis in PCP mutants. This study therefore identifies PCP as the initial symmetry-breaking signal for the planar polarization of PAR proteins in asymmetrically dividing SOPs. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Digestive ripening: a synthetic method par excellence for core–shell ...

    Indian Academy of Sciences (India)

    Digestive ripening: a synthetic method par excellence for core–shell, alloy, and composite nanostructured materials. SRILAKSHMI P BHASKAR and BALAJI R JAGIRDAR. ∗. Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore 560 012, India e-mail: jagirdar@ipc.iisc.ernet.in. Abstract.

  12. ParA encoded on chromosome II of Deinococcus radiodurans binds ...

    Indian Academy of Sciences (India)

    2013-07-22

    Jul 22, 2013 ... detected using NBT/BCIP (Roche Biochemical) colour reagent using the manufacturer's protocol. 2.6 Measurements of ATPase and GTPase activities. ATPase activity of recombinant ParA2 was checked using a modified protocol as described in (Kota et al. 2010). In brief, the increasing concentrations of ...

  13. Ambroise Paré (1510-1590)--Traek af kirurgfagets historie og dets medi- kamentelle aspekter

    DEFF Research Database (Denmark)

    Norn, Svend; Permin, Henrik; Kruse, Poul R

    2010-01-01

    the painful practise of cautery to stop bleeding and used ligatures and dressings instead. A multitude of subjects were included in his writings such as military surgery, aneurysm, hernia, obstetrics and plague, and through his techniques he guided the development of the gentle art of surgery. Paré became...

  14. Tuberculosis prevalence and risk factors for water buffalo in Pará, Brazil.

    Science.gov (United States)

    Barbosa, José D; da Silva, Jenevaldo B; Rangel, Charles P; da Fonseca, Adivaldo H; Silva, Natália S; Bomjardim, Henrique A; Freitas, Nayra F Q R

    2014-03-01

    The prevalence of and possible risk factors for tuberculosis were studied in water buffalo from Pará, Brazil. In this study, 3,917 pregnant and nonpregnant female Murrah and Mediterranean buffaloes were studied; 2,089 originated from Marajó Island, and 1,108 were from the mainland. The comparative cervical tuberculin test was used as a diagnostic test for tuberculosis in these animals. The prevalence of positive buffaloes was 3.5 % (100/2,809) on Marajó Island and 7.2 % (80/1,108) on the mainland. The municipalities with the highest tuberculosis prevalence rates in animals were Ipixuna do Pará (10.1 %), Marapanim (9.8 %), Chaves (9.4 %), Paragominas (8.6 %), and Cachoeira do Arari (6.7 %). The tuberculosis prevalence was not significantly different between the Murrah (4.3 %) and Mediterranean (4.8 %) breeds or between pregnant (5 %) and nonpregnant (4.3 %) buffaloes. Tuberculosis was detected in water buffaloes from Pará, Brazil; the mainland buffalo exhibited the highest tuberculosis prevalence. These results indicate that this disease is dangerous to public health and buffalo farming in Pará.

  15. Jesus en geweld: Markus 12:1-12 (en par) en Thomas 65 | van Eck ...

    African Journals Online (AJOL)

    Jesus and violence: Mark 12:1-12 (and par) and. Thomas 65. As an advanced agrarian (aristocratic) society, first-century Palestine's social fabric was built on systemic tensions and conflicts between the elite (rulers) and the peasantry (the ruled). At the root of these conflicts were incompatible values (ideologies) and

  16. Innate and adaptive immune responses to the major Parietaria allergen Par j 1 in healthy subjects.

    Science.gov (United States)

    Bonura, A; Quaratino, S; Gervasi, F; Melis, M R; Di Sano, C; Colombo, P

    2013-07-01

    In this study we wanted to analyse the pattern of the immune response to the Parietaria major allergen Par j 1 in freshly purified peripheral blood mononuclear cell (PBMC) from healthy subjects. We observed that Par j 1 was capable of inducing IFN-γ production by CD3⁻ and CD16⁺/CD56⁺ cells exclusively in healthy individuals. Furthermore, a multiparametric analysis allowed us a better definition of two IFN-γ-Par j 1 specific populations (IFN-γ(dim) and IFN-γ(high)) characterized by the presence of different proportions of NKT and NK cells. We also identified the concomitant presence of a subset of IL-10⁺ NK cells. Moreover, CFSE staining showed that the Par j 1 preferentially induced the proliferation of CD3⁻/CD56⁺/CD335⁺ cells. Finally, a subset of CD4⁺/CD25⁺/FoxP3⁺/IL-10⁻ T cells was identified. The result of this pilot study suggest that during a tolerogenic response, the major allergen of the Parietaria pollen works as an activator of both the innate and the adaptive human immune system. Copyright © 2012 Elsevier GmbH. All rights reserved.

  17. Granulome conjonctival suite à un traumatisme par épine végétale ...

    African Journals Online (AJOL)

    Granulome conjonctival suite à un traumatisme par épine végétale négligé: à propos d'un cas. Taha Elghazi, Amine Eljai, Maryama Elkaddoumi, Omar Lazrek, Sofia Hassani Saoudi, Taib Belkbir, Abdellah Amazouzi, Lalla Ouafae Cherkaoui, Rajae Daoudi ...

  18. Parásitos de animales silvestres en cautiverio en Lima, Perú

    Directory of Open Access Journals (Sweden)

    Lily Arrojo

    2013-06-01

    Full Text Available Los parásitos de los animales silvestres en cautiverio peruanos se registran aquí. La mayoría de las especies son nematodos. Dos especies son al patógeno de host: Prosthenorchois elegans y Strongyloides sp.

  19. Compression robuste du signal EMG par la transformee avec lec B ...

    African Journals Online (AJOL)

    Compression robuste du signal EMG par la transformee avec lec B-splines. PN Eloundou, P Ele, E Tonye. Abstract. No Abstract. Journal of the Cameroon Academy of Sciences Vol. 6 (3) 2006: pp. 195-206. Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT.

  20. CRP and suPAR are differently related to anthropometry and subclinical organ damage

    DEFF Research Database (Denmark)

    Lyngbæk, Stig; Sehestedt, Thomas Berend; Marott, Jacob Louis

    2013-01-01

    BACKGROUND: Low-grade inflammation is a marker for cardiovascular disease (CVD). The inflammatory biomarkers C-reactive protein (CRP) and soluble urokinase plasminogen activator receptor (suPAR) independently predict CVD. We tested the hypothesis that these biomarkers reflect different aspects...

  1. 99 Modélisation de la pollution par les nitrates de la nappe de ...

    African Journals Online (AJOL)

    AKA BOKO

    Modélisation de la pollution par les nitrates de la nappe de. Berrechid, au Maroc. Karima EL BOUQDAOUI1*, Mostafa AACHIB2, Mohammed BLAGHEN1 et. Sanaa KHOLTEI3. 1Université Hassan II, Faculté des Sciences, Département biologie, Ain Chock,. B.P. 5366, Maârif, Casablanca, Maroc. 2 Ecole Hassania des ...

  2. Determining fPAR and leaf area index of several land cover classes ...

    African Journals Online (AJOL)

    Determining the quantum (both annual maxima and minima) and the temporal variation in the leaf area index (LAI), and the fraction of photosynthetically active radiation (fPAR), are three fundamental biophysical characteristics of the plant canopy that should parameterise ecophysiological models of water use ...

  3. CALiPER Report 20.3: Robustness of LED PAR38 Lamps

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2014-12-30

    A small sample of each of the CALiPER Application Summary Report 20 PAR38 lamp types underwent stress testing that included substantial temperature and humidity changes, electrical variation, and vibration. The results do not directly address expected lifetime, but can be compared with one another, as well as with benchmark conventional products, to assess the relative robustness of the product designs.

  4. Development of a par-boiled rice solar dryer | Abubakar | Bayero ...

    African Journals Online (AJOL)

    Energy is required in various forms to do useful work and necessary for the continual improvement in the standard of any society. This study presents the development of a par-boiled batch type rice solar dryer using an indirect mode natural convection solar energy. The dryer was designed and constructed to provide easier, ...

  5. ACUTE INTRAOPERATIVE SUPRACHOROIDAL HEMORRHAGE DURING SMALL-GAUGE PARS PLANA VITRECTOMY.

    Science.gov (United States)

    Sukpen, Intira; Stewart, Jay M

    2017-11-16

    To report the intraoperative occurrence of acute intraoperative suprachoroidal hemorrhage during small-gauge pars plana vitrectomy. A review of a surgical patient who developed acute intraoperative suprachoroidal hemorrhage during small-gauge pars plana vitrectomy because of bucking under general anesthesia. A 32-year-old obese woman with proliferative diabetic retinopathy and traction retinal detachment in the left eye who developed intraoperative suprachoroidal hemorrhage during small-gauge pars plana vitrectomy because of bucking under general anesthesia while doing endolaser under air infusion. The pressure was immediately elevated to stabilize the hemorrhage without sclerotomy creation, and then gas (14% C3F8) was injected, with subsequent face-down positioning. The patient subsequently developed proliferative vitreoretinopathy, requiring additional surgery. The final visual acuity at 14-month follow-up was hand motions with a reattached retina in the left eye. Valsalva-induced suprachoroidal hemorrhage during pars plana vitrectomy under general anesthesia may result in sight-threatening visual consequences. Current small-gauge vitrectomy techniques using valved cannulas may allow for better intraoperative management of this complication through control of the intraocular pressure in a closed system.

  6. Participation and Participatory Action Research (PAR) in Environmental Education Processes: For What Are People Empowered?

    Science.gov (United States)

    Le Grange, Lesley

    2009-01-01

    Participatory action research (PAR) derived from anti-colonial struggles in the third world in the 1960s. Traditionally it has been a method of the margins because of its commitment to linking social justice to research. Because of its counter-hegemonic tendency it has had great appeal among environmental educators advocating a socially critical…

  7. Over de musculatuur van de pars intramularis tubae fallopii en haar functionele betekenis.

    NARCIS (Netherlands)

    Vasen, Ludwig Christiaan Leon Marie

    1959-01-01

    Frequent attempts have been made to discover in the region of the pars intramuralis tubae a sphinctermechanism, which might impede or even prevent the passage of noxious material from the lumen of the uterus to the abdominal cavity. Considerable time ago, anatomical structures were observed in

  8. The Utility of Clinicians Ratings of Anxiety Using the Pediatric Anxiety Rating Scale (PARS)

    Science.gov (United States)

    Ginsburg, Golda S.; Keeton, Courtney P.; Drazdowski, Tess K.; Riddle, Mark A.

    2011-01-01

    Clinician ratings of anxiety hold the promise of clarifying discrepancies often found between child and parent reports of anxiety. The Pediatric Anxiety Rating Scale (PARS) is a clinician-administered instrument that assesses the frequency, severity, and impairment of common pediatric anxiety disorders and has been used as a primary outcome…

  9. Tratamiento exitoso de un hemangioma de parótida con propranolol

    National Research Council Canada - National Science Library

    Edelisa Moredo Romo; Fernanda Pastrana Fundora

    2012-01-01

    .... Se presenta el caso clínico de un paciente con diagnóstico de hemangioma de parótida con componente superficial y profundo, tratado con propranolol, con excelente respuesta al tratamiento, involución completa clínica...

  10. Par@Graph - a parallel toolbox for the construction and analysis of large complex climate networks

    NARCIS (Netherlands)

    Tantet, A.J.J.

    2015-01-01

    In this paper, we present Par@Graph, a software toolbox to reconstruct and analyze complex climate networks having a large number of nodes (up to at least 106) and edges (up to at least 1012). The key innovation is an efficient set of parallel software tools designed to leverage the inherited hybrid

  11. Optimisation de la production d'éthanol par les techniques d ...

    African Journals Online (AJOL)

    hydrolyse de l'amidon. Il vaut mieux produire l'éthanol à partir d'une fermentation contrôlée pour éviter les intoxications provoquées par le méthanol. Mots clés: fermentation alcoolique, Saccharomyces cerevisiae, amidon de ...

  12. uPAR-targeted multimodal tracer for pre- and intraoperative imaging in cancer surgery

    NARCIS (Netherlands)

    Boonstra, M.C.; Van Driel, P.B.A.A.; Van Willigen, D.M.; Stammes, M.A.; Prevoo, H.A.J.M.; Tummers, Q.R.J.G.; Mazar, A.P.; Beekman, F.J.; Kuppen, P.J.K.; Van de Velde, C.J.H.; Löwik, C.W.G.M.; Frangioni, J.V.; Van Leeuwen, F.W.B.; Sier, C.F.M.; Vahrmeijer, A.L.

    2015-01-01

    Pre- and intraoperative diagnostic techniques facilitating tumor staging are of paramount importance in colorectal cancer surgery. The urokinase receptor (uPAR) plays an important role in the development of cancer, tumor invasion, angiogenesis, and metastasis and over-expression is found in the

  13. Une occlusion intestinale aiguë par un os de poulet | Mahmoudi ...

    African Journals Online (AJOL)

    Il s'agit le plus souvent d'un accident survenant chez des enfants, parfois chez des malades psychiatriques. Chez les adultes, l'accident est favorisé par des mauvaises habitudes alimentaires (repas trop rapide, quintes de toux ou dispute lors du repas...). L'introduction ... Les suites opératoires étaient simples. Pan African ...

  14. Funding Research Through the Online Partnership to Accelerate Research (OnPAR

    Directory of Open Access Journals (Sweden)

    Martin A. Dueñas, MPA

    2016-10-01

    Full Text Available OnPAR—the Online Partnership to Accelerate Research—seeks to provide a second opportunity for funding of high-quality, unfunded applications originally submitted to the National Institutes of Health and other national and international funding agencies. OnPAR will match applicable, unfunded applications with the research priorities of nongovernment organizations such as private biomedical foundations, pharmaceutical companies, venture capital funds, and other private funds. Funding organization members will review and make final funding decisions through a simple, 2-step process whereby applicants can submit public abstracts directly to OnPAR. If a member requests additional information, then, by invitation only, an applicant can submit their original unfunded application and their peer review summary statement. Advancing research discovery and drug development to improve clinical outcomes for patients afflicted with or at risk for disease is the primary goal of OnPAR. OnPAR invites the scientific community to fully participate in this new funding paradigm by submitting their National Institutes of Health public abstracts so that funding members can review and potentially support these high-quality, unfunded applications.

  15. Tuberculose de l'épaule masquée par une infection concomitante à ...

    African Journals Online (AJOL)

    Tuberculose de l'épaule masquée par une infection concomitante à enterobacter cloacae: à propos d'un cas. Mariam Gbané-Koné, Samba Koné, Boubacar Ouali, Kouassi Jean-Mermoz Djaha, Mohamed Diomandé, Edmond Eti, Stanislas André Touré, N'zué Marcel Kouakou ...

  16. Préparation des charbons actifs par voie chimique à l'acide ...

    African Journals Online (AJOL)

    Les matieres ligno-cellulosiques sont des precurseurs du charbon actif. Le present travail a eu pour but la preparation de charbons actifs a partir des coques de noix de coco par activation chimique a lfacide phosphorique. Pour optimiser le procede de la preparation, les effets des concentrations de lfacide phosphorique ...

  17. Elimination du cadmium de l'acide phosphorique par électrodialyse ...

    African Journals Online (AJOL)

    homme; elle est devenue un enjeu économique et politique majeur pour le développement durable des pays. L'acide phosphorique est classé en second lieu dans la production mondiale des acides après l'acide sulfurique. Il est fabriqué par ...

  18. Que sont devenues les plantes autrefois consommées par les divers ...

    African Journals Online (AJOL)

    Que sont devenues les plantes autrefois consommées par les divers groupes ethnoculturels du Togo? S Akpavi, AY Woegan, M Dourma, K Tozo, K Batawila, K Wala, KA Gbogbo, M Kanda, K Kossi-Titrikou, B de Foucault, I Butare, K Akpagana ...

  19. La bio-indication de la pollution aquatique par les microalgues (Cas ...

    African Journals Online (AJOL)

    JS.II. Resume. Les milieux aquatiques continentaux sont des ecosystemes complexes, fragiles et regis par des lois physico-chimiques precises. Les microalgues qui les colonisent vivent en equilibre instable et en dependent totalement. Les genres les plus tolerants s'adaptent a l'entree d'une substance exogene ou a une ...

  20. Purification and characterization of Par o I, major allergen of Parietaria officinalis pollen.

    Science.gov (United States)

    Oreste, U; Coscia, M R; Scotto d'Abusco, A; Santonastaso, V; Ruffilli, A

    1991-01-01

    Par o I, a major allergen of Parietaria officinalis, was purified from the pollen extract. The purified allergen was obtained by ultrafiltration, Sephadex gel filtration and DE-52 ion exchange chromatography: the purified preparation yields a single band in polyacrylamide gel isoelectric focusing (PAG-IEF), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting, a single immunoprecipitation arc in crossed immunoelectrophoresis (CIE) and crossed radioimmunoelectrophoresis (CRIE) and a single peak in size exclusion high-performance liquid chromatography (HPLC). Par o I is a glycoprotein with a protein to carbohydrate ratio of 100:21. The molecular weight, determined by SDS-PAGE, Sephadex G-50 gel filtration and size exclusion HPLC, varied between 13.5 and 14.5 kDa according to the method employed. The isoelectric point was 4.6. The amino acid composition and the sequence of the first twelve N-terminal residues were determined. The allergenicity was assayed in vivo and in vitro. 29/29 Parietaria-allergic patients were skin positive to Par o I and possessed high level of specific serum IgE antibody as it determined by radioallergosorbent test (RAST). Par o I contained dominant epitopes for human IgE as inhibited to 85% the pollen extract RAST performed with a pool of sera of allergic patients. The RAST inhibitory activity was not abolished by deglycosylation.

  1. A 3-Year Review of the Outcome of Pars Plana Vitrectomy for ...

    African Journals Online (AJOL)

    A 3-Year Review of the Outcome of Pars Plana Vitrectomy for Dropped Lens Fragments after Cataract Surgery in a Tertiary Eye Hospital in Dhaka, Bangladesh. Lateefat Olokoba ... Details of intra ocular procedure (Cryotherapy, Endo laser, Silicon oil or gas tamponade) in addition to PPV were noted. RESULT: Thirty- two ...

  2. ParA encoded on chromosome II of Deinococcus radiodurans binds ...

    Indian Academy of Sciences (India)

    These results suggested that ParA2 is a nucleoid-binding protein, which could inhibits cell division in E. coli by affecting the correct localization of FtsZ and thereby cytokinesis. Helping slmAminCDE mutant to produce minicells, a phenotype associated with mutations in the `Min' proteins, further indicated the possibility of ...

  3. The interaction between uPAR and vitronectin triggers ligand-independent adhesion signalling by integrins

    Science.gov (United States)

    Ferraris, Gian Maria Sarra; Schulte, Carsten; Buttiglione, Valentina; De Lorenzi, Valentina; Piontini, Andrea; Galluzzi, Massimiliano; Podestà, Alessandro; Madsen, Chris D; Sidenius, Nicolai

    2014-01-01

    The urokinase-type plasminogen activator receptor (uPAR) is a non-integrin vitronectin (VN) cell adhesion receptor linked to the plasma membrane by a glycolipid anchor. Through structure–function analyses of uPAR, VN and integrins, we document that uPAR-mediated cell adhesion to VN triggers a novel type of integrin signalling that is independent of integrin–matrix engagement. The signalling is fully active on VN mutants deficient in integrin binding site and is also efficiently transduced by integrins deficient in ligand binding. Although integrin ligation is dispensable, signalling is crucially dependent upon an active conformation of the integrin and its association with intracellular adaptors such as talin. This non-canonical integrin signalling is not restricted to uPAR as it poses no structural constraints to the receptor mediating cell attachment. In contrast to canonical integrin signalling, where integrins form direct mechanical links between the ECM and the cytoskeleton, the molecular mechanism enabling the crosstalk between non-integrin adhesion receptors and integrins is dependent upon membrane tension. This suggests that for this type of signalling, the membrane represents a critical component of the molecular clutch. PMID:25168639

  4. Structure and ligand interactions of the urokinase receptor (uPAR)

    DEFF Research Database (Denmark)

    Kjaergaard, Magnus; Hansen, Line V.; Jacobsen, Benedikte

    2008-01-01

    The urokinase-type plasminogen activator receptor (uPAR or CD87) is a glycolipid-anchored membrane glycoprotein, which is responsible for focalizing plasminogen activation to the cell surface through its high-affinity binding to the serine protease uPA. This tight interaction (KD less than 1 nM) ...

  5. PAR as a way of organising a social workers labour union in Greenland

    DEFF Research Database (Denmark)

    Andersen, John; Arnfjord, Steven

    Abstract, ALARA2015012:PAR as a way of organising a social workers labour union in Greenland for consideration and inclusion in the programme for the ALARA 9th Action Learning Action Research and 13th Participatory Action Research World Congress to be held at St. George Hotel and Conference Centre...

  6. Usefulness of suPAR as a biological marker in patients with systemic inflammation or infection: a systematic review

    NARCIS (Netherlands)

    Backes, Yara; van der Sluijs, Koenraad F.; Mackie, David P.; Tacke, Frank; Koch, Alexander; Tenhunen, Jyrki J.; Schultz, Marcus J.

    2012-01-01

    Systemic levels of soluble urokinase-type plasminogen activator receptor (suPAR) positively correlate with the activation level of the immune system. We reviewed the usefulness of systemic levels of suPAR in the care of critically ill patients with sepsis, SIRS, and bacteremia, focusing on its

  7. Prognostic significance of APACHE II score and plasma suPAR in Chinese patients with sepsis: a prospective observational study.

    Science.gov (United States)

    Liu, Xuan; Shen, Yong; Li, Zhihua; Fei, Aihua; Wang, Hairong; Ge, Qinmin; Pan, Shuming

    2016-07-29

    Timely risk stratification is the key strategy to improve prognosis of patients with sepsis. Previous study has proposed to develop a powerful risk assessment rule by the combination of Acute Physiology and Chronic Health Evaluation II (APACHE II) score and plasma soluble urokinase plasminogen activator receptor (suPAR). That reaffirmation of suPAR as a prognostic marker in Chinese patients with severe sepsis is the aim of the study. A total of 137 consecutive Chinese patients with sepsis were enrolled in a prospective study cohort. Demographic and clinical characteristics, conventional risk factors and important laboratory data were prospectively recorded. Sequential plasma suPAR concentrations were measured by an enzymeimmunoabsorbent assay on days 1, 3, and 7 after admission to the intensive care unit (ICU). Receiver operating characteristic (ROC) curves and Cox regression analysis were used to examine the performance of suPAR in developing a rule for risk stratification. The results showed that plasma suPAR concentrations remained relatively stable within survivors and non-survivors during the first week of disease course. Regression analysis indicated that APACHE II ≥15 and suPAR ≥10.82 ng/mL were independently associated with unfavorable outcome. With the above cutoffs of APACHE II and suPAR, strata of disease severity were determined. The mortality of each stratum differed significantly from the others. Combination of APACHE II score and suPAR may supply the powerful prognostic utility for the mortality of sepsis.

  8. Rational targeting of the urokinase receptor (uPAR): development of antagonists and non-invasive imaging probes

    DEFF Research Database (Denmark)

    Kriegbaum, M C; Persson, M; Haldager, L

    2011-01-01

    -ray crystallography and surface plasmon resonance studies. Importantly, these structural studies also define possible druggable target sites in uPAR for small molecules and provide guidelines for the development of reporter groups applicable for non-invasive molecular imaging of uPAR expression in vivo by positron...

  9. AP-3 regulates PAR1 ubiquitin-independent MVB/lysosomal sorting via an ALIX-mediated pathway.

    Science.gov (United States)

    Dores, Michael R; Paing, May M; Lin, Huilan; Montagne, William A; Marchese, Adriano; Trejo, JoAnn

    2012-09-01

    The sorting of signaling receptors within the endocytic system is important for appropriate cellular responses. After activation, receptors are trafficked to early endosomes and either recycled or sorted to lysosomes and degraded. Most receptors trafficked to lysosomes are modified with ubiquitin and recruited into an endosomal subdomain enriched in hepatocyte growth factor-regulated tyrosine kinase substrate (HRS), a ubiquitin-binding component of the endosomal-sorting complex required for transport (ESCRT) machinery, and then sorted into intraluminal vesicles (ILVs) of multivesicular bodies (MVBs)/lysosomes. However, not all receptors use ubiquitin or the canonical ESCRT machinery to sort to MVBs/lysosomes. This is exemplified by protease-activated receptor-1 (PAR1), a G protein-coupled receptor for thrombin, which sorts to lysosomes independent of ubiquitination and HRS. We recently showed that the adaptor protein ALIX binds to PAR1, recruits ESCRT-III, and mediates receptor sorting to ILVs of MVBs. However, the mechanism that initiates PAR1 sorting at the early endosome is not known. We now report that the adaptor protein complex-3 (AP-3) regulates PAR1 ubiquitin-independent sorting to MVBs through an ALIX-dependent pathway. AP-3 binds to a PAR1 cytoplasmic tail-localized tyrosine-based motif and mediates PAR1 lysosomal degradation independent of ubiquitination. Moreover, AP-3 facilitates PAR1 interaction with ALIX, suggesting that AP-3 functions before PAR1 engagement of ALIX and MVB/lysosomal sorting.

  10. Manufactering of par-fried french-fries. Part 3: a blueprint to predict the maximum production yield

    NARCIS (Netherlands)

    Somsen, D.J.; Capelle, A.; Tramper, J.

    2004-01-01

    Very little research on the production yield of par-fried French-fries has been reported in the literature. This paper bridges the knowledge gap and outlines the development of a model to predict the maximum production yield of par-fried French-fries. This yield model can be used to calculate the

  11. Pollution bactériologique des puits d'eau utilisés par les populations ...

    African Journals Online (AJOL)

    Les analyses des coliformes ont été faites suivant la technique par inclusion en une couche, et celle des streptocoques par ensemencement dans les tubes à vis avec bouillon de Rothe. Les résultats montrent que ces eaux abritent de nombreuses bactéries pathogènes, coliformes fécaux et streptocoques fécaux.

  12. Project Plan 7930 Cell G PaR Remote Handling System Replacement

    Energy Technology Data Exchange (ETDEWEB)

    Kinney, Kathryn A [ORNL

    2009-10-01

    For over 40 years the US Department of Energy (DOE) and its predecessors have made Californium-252 ({sup 252}Cf) available for a wide range of industries including medical, nuclear fuels, mining, military and national security. The Radiochemical Engineering Development Center (REDC) located within the Oak Ridge National Laboratory (ORNL) processes irradiated production targets from the High Flux Isotope Reactor (HFIR). Operations in Building 7930, Cell G provide over 70% of the world's demand for {sup 252}Cf. Building 7930 was constructed and equipped in the mid-1960s. Current operations for {sup 252}Cf processing in Building 7930, Cell G require use of through-the-wall manipulators and the PaR Remote Handling System. Maintenance and repairs for the manipulators is readily accomplished by removal of the manipulator and relocation to a repair shop where hands-on work can be performed in glove boxes. Contamination inside cell G does not currently allow manned entry and no provisions were created for a maintenance area inside the cell. There has been no maintenance of the PaR system or upgrades, leaving operations vulnerable should the system have a catastrophic failure. The Cell G PaR system is currently being operated in a run to failure mode. As the manipulator is now 40+ years old there is significant risk in this method of operation. In 2006 an assessment was completed that resulted in recommendations for replacing the manipulator operator control and power centers which are used to control and power the PaR manipulator in Cell G. In mid-2008 the chain for the bridge drive failed and subsequent examinations indicated several damaged links (see Figure 1). To continue operations the PaR manipulator arm is being used to push and pull the bridge as a workaround. A retrieval tool was fabricated, tested and staged inside Cell G that will allow positioning of the bridge and manipulator arm for removal from the cell should the PaR system completely fail. A fully

  13. Radiological findings and healing patterns of incomplete stress fractures of the pars interarticularis

    Energy Technology Data Exchange (ETDEWEB)

    Dunn, Andrew J.; Campbell, Robert S.D. [Royal Liverpool and Broadgreen University Teaching Hospitals, Department of Medical Imaging, Liverpool (United Kingdom); Mayor, Peter E. [Leighton Hospital, Department of Medical Imaging, Crewe, Cheshire (United Kingdom); Rees, Dai [Robert Jones and Agnes-Hunt Orthopaedic Hospital, Department of Orthopaedic Surgery, Oswestry, Shropshire (United Kingdom)

    2008-05-15

    The objective was to retrospectively record the CT and MRI features and healing patterns of acute, incomplete stress fractures of the pars interarticularis. The CT scans of 156 adolescents referred with suspected pars interarticularis stress fractures were reviewed. Patients with incomplete (grade 2) pars fractures were included in the study. Fractures were assessed on CT according to vertebral level, location of cortical involvement and direction of fracture propagation. MRI was also performed in 72 of the 156 cases. MRI images of incomplete fractures were assessed for the presence of marrow oedema and cortical integrity. Fracture healing patterns were characterised on follow-up CT imaging. Twenty-five incomplete fractures were identified in 23 patients on CT. All fractures involved the inferior or infero-medial cortex of the pars and propagated superiorly or superolaterally. Ninety-two percent of incomplete fractures demonstrated either complete or partial healing on follow-up imaging. Two (8%) cases progressed to complete fractures. Thirteen incomplete fractures in 11 patients confirmed on CT also had MRI, and 92% demonstrated oedema in the pars. Ten out of thirteen fractures (77%) showed a break in the infero-medial cortex with intact supero-lateral cortex, which correlated with the CT findings. MRI incorrectly graded one case as a complete (grade 3) fracture, and 2 cases as (grade 1) stress reaction. Six fractures had follow-up MRI, 67% showed partial or complete cortical healing, and the same number showed persistent marrow oedema. Incomplete fracture of the pars interarticularis represents a stage of the evolution of a complete stress fracture. The direction of fracture propagation is consistent, and complete healing can be achieved in most cases with appropriate clinical management. CT best demonstrates fracture size and extent, and is the most appropriate modality for follow-up. MRI is limited in its ability to fully depict the cortical integrity of

  14. Plant-derived SAC domain of PAR-4 (Prostate Apoptosis Response 4 exhibits growth inhibitory effects in prostate cancer cells

    Directory of Open Access Journals (Sweden)

    Shayan eSarkar

    2015-10-01

    Full Text Available The gene Par-4 (Prostate Apoptosis Response 4 was originally identified in prostate cancer cells undergoing apoptosis and its product Par-4 showed cancer specific pro-apoptotic activity. Particularly, the SAC domain of Par-4 (SAC-Par-4 selectively kills cancer cells leaving normal cells unaffected. The therapeutic significance of bioactive SAC-Par-4 is enormous in cancer biology; however, its large scale production is still a matter of concern. Here we report the production of SAC-Par-4-GFP fusion protein coupled to translational enhancer sequence (5′ AMV and apoplast signal peptide (aTP in transgenic Nicotiana tabacum cv. Samsun NN plants under the control of a unique recombinant promoter M24. Transgene integration was confirmed by genomic DNA PCR, Southern and Northern blotting, Real-time PCR and Nuclear run-on assays. Results of Western blot analysis and ELISA confirmed expression of recombinant SAC-Par-4-GFP protein and it was as high as 0.15% of total soluble protein. In addition, we found that targeting of plant recombinant SAC-Par-4-GFP to the apoplast and endoplasmic reticulum (ER was essential for the stability of plant recombinant protein in comparison to the bacterial derived SAC-Par-4. Deglycosylation analysis demonstrated that ER-targeted SAC-Par-4-GFP-SEKDEL undergoes O-linked glycosylation unlike apoplast-targeted SAC-Par-4-GFP. Furthermore, various in vitro studies like mammalian cells proliferation assay (MTT, apoptosis induction assays, and NF-κB suppression suggested the cytotoxic and apoptotic properties of plant-derived SAC-Par-4-GFP against multiple prostate cancer cell lines. Additionally, pre-treatment of MAT-LyLu prostate cancer cells with purified SAC-Par-4-GFP significantly delayed the onset of tumor in a syngeneic rat prostate cancer model. Taken altogether, we proclaim that plant made SAC-Par-4 may become a useful alternate therapy for effectively alleviating cancer in the new era.

  15. Prognosis in adenocarcinomas of lower oesophagus, gastro-oesophageal junction and cardia evaluated by uPAR-immunohistochemistry

    DEFF Research Database (Denmark)

    Laerum, Ole Didrik; Ovrebo, Kjell; Skarstein, Arne

    2012-01-01

    Adenocarcinomas of lower oesophagus, gastro-oesophageal junction and cardia in humans are highly invasive tumours with poor prognosis. The localisation of urokinase-type plasminogen activator receptor (uPAR) was determined in 66 patients; 60 with adenocarcinomas and six cases with Barrett......'s oesophagus. uPAR was expressed in nearly all cases of invasive adenocarcinomas by populations of cancer cells, macrophages and myofibroblasts at both the invasion front and the tumour core. In areas with high-grade dysplasia or with Barrett's metaplasia adjacent to the tumour tissue, no u......PAR-positivity on immmunohistochemically stained specimens, a significant association was found between poor overall survival and high uPAR-score for cancer cells in the tumour core and for macrophages peripherally at the tumour invasion zone. In multivariate analysis, these two uPAR-scores were confirmed as highly significant prognostic...

  16. RAPID MACULAR HOLE FORMATION, SPONTANEOUS CLOSURE, AND REOPENING AFTER PARS PLANA VITRECTOMY FOR MACULA-SPARING RETINAL DETACHMENT.

    Science.gov (United States)

    Sridhar, Jayanth; Townsend, Justin H; Rachitskaya, Aleksandra V

    2017-01-01

    To report a single case of macular hole (MH) opening, spontaneous closure, and reopening in the 3-month period after pars plana vitrectomy for rhegmatogenous retinal detachment (RRD). Case report. A 59-year-old man with a macula-sparing RRD underwent uncomplicated pars plana vitrectomy with gas tamponade. The patient developed a FTMH 2 months postoperatively. The hole was noted to spontaneously close 3 months postoperatively, but then reopened 4 months postoperatively and required repeat pars plana vitrectomy and inner limiting membrane peel for definitive closure. The rapidity with which the MH developed after pars plana vitrectomy for macula-sparing RRD and its dynamic behavior have not been previously reported. Full-thickness macular holes may rarely develop rapidly, spontaneous close, and reopen after pars plana vitrectomy for RRD, even in macula-sparing cases.

  17. Percutaneous Direct Repair of a Pars Defect Using Intraoperative Computed Tomography Scan: A Modification of the Buck Technique.

    Science.gov (United States)

    Nourbakhsh, Ali; Preuss, Fletcher; Hadeed, Michael; Shimer, Adam

    2017-06-01

    Case report. To describe a young adult with a pars defect undergoing percutaneous direct fixation using intraoperative computed tomography (CT) scan. Direct pars repair has been utilized since the 1960s. There are no reports in the literature describing a percutaneous technique. Using a percutaneous technique under the guide of intraoperative CT scan, a cannulated partially threaded screw was inserted across the pars defect. Surgery was completed without complication and the patient returned to preoperative activity level 3 months post-op. Postoperative CT scan showed a well-healed L4 pars defect. Percutaneous direct pars repair using intraoperative CT scan offers the advantage of minimal soft tissue dissection, thereby reducing blood loss, infection risk, and recovery time. 5.

  18. Un encadrement éthique du TDM par une « charte éthique »

    OpenAIRE

    Direction de l’Information Scientifique et Technique - CNRS,

    2017-01-01

    Les résultats obtenus par TDM doivent évidemment être interprétés et analysés avec un esprit critique. Un risque serait, par exemple, d’établir des liens de causalité abusifs pour expliquer les statistiques obtenues par TDM, menant à des corrélations fallacieuses. Le TDM ouvre ainsi des nouvelles questions méthodologiques. L’intégrité de la recherche par TDM passe également par un respect de règles d’éthique. En particulier, le traitement des données personnelles doit s’effectuer dans le resp...

  19. Fabrication et caracterisation de nanocristaux de silicium localises, realises par gravure electrochimique pour des applications nanoelectroniques

    Science.gov (United States)

    Ayari-Kanoun, Asma

    Ce travail de these porte sur le developpement d'une nouvelle approche pour la localisation et l'organisation de nanocristaux de silicium realises par gravure electrochimique. Cette derniere represente une technique simple et peu couteuse par rapport aux autres techniques couramment utilisees pour la fabrication de nanocristaux de silicium. L'idee de ce travail a ete d'etudier la nanostructuration de minces couches de nitrure de silicium, d'environ 30 nm d'epaisseur pour permettre par la suite un arrangement periodique des nanocristaux de silicium. Cette pre-structuration est obtenue de facon artificielle en imposant un motif periodique via une technique de lithographie par faisceau d'electrons combinee avec une gravure plasma. Une optimisation des conditions de lithographie et de gravure plasma ont permis d'obtenir des reseaux de trous de 30 nm de diametre debouchant sur le silicium avec un bon controle de leur morphologie (taille, profondeur et forme). En ajustant les conditions de gravure electrochimique (concentration d'acide, temps de gravure et densite de courant), nous avons obtenu des reseaux -2D ordonnes de nanocristaux de silicium de 10 nm de diametre a travers ces masques de nanotrous avec le controle parfait de leur localisation, la distance entre les nanocristaux et leur orientation cristalline. Des etudes electriques preliminaires sur ces nanocristaux ont permis de mettre en evidence des effets de chargement. Ces resultats tres prometteurs confirment l'interet des nanocristaux de silicium realises par gravure electrochimique dans le futur pour la fabrication a grande echelle de dispositifs nanoelectroniques. Mots-cles : localisation, organisation, nanocristaux de silicium, gravure electrochimique, lithographie electronique, gravure plasma, nitrure de silicium.

  20. Outcomes of pars plana glaucoma drainage implant in Boston type 1 keratoprosthesis surgery.

    Science.gov (United States)

    Huh, Eun S; Aref, Ahmad A; Vajaranant, Thasarat S; de la Cruz, Jose; Chau, Felix Y; Cortina, Maria S

    2014-01-01

    Glaucoma drainage implantation in conjunction with Boston Type 1 Keratoprosthesis placement is a surgical option in controlling postoperative glaucoma. The purpose of this study is to report outcomes of combined pars plana vitrectomy and glaucoma drainage implantation with corneal patch graft in Boston Type 1 Keratoprosthesis patients. A retrospective review of patients who underwent pars plana glaucoma drainage implantation in combination with Boston Type 1 Keratoprosthesis was performed. Preoperative and postoperative parameters collected and analyzed included: visual acuity, intraocular pressure, number of glaucoma medications to achieve intraocular pressure control, bandage contact lens fit, and postoperative complications. Twenty eyes of 20 patients were identified; 95% had preoperative diagnosis of glaucoma, utilizing on an average 2.5 medications with an average intraocular pressure of 19.8 mm Hg (±6.3 mm Hg; range, 9 to 32.8 mm Hg). After placement of the pars plana glaucoma drainage implant, an average intraocular pressure of 19 mm Hg (±7.0 mm Hg; range, 8 to 30 mm Hg) by scleral pneumotonometry was achieved and 85% were deemed to have normal pressures by digital palpation. An average of 2.1 intraocular pressure-lowering medications were required on last follow-up. Average follow-up was 31.6 months (±17.4 mo; range, 12.3 to 71.6 mo). Two eyes required glaucoma drainage implant explantation: one eye due to endophthalmitis from a nonhealing corneal ulcer and the other eye due to corneal melt. None of our patients experienced conjunctival erosion over a pars plana positioned glaucoma drainage implant or tube. For the long-term management of glaucoma in keratoprosthesis patients, a posteriorly placed pars plana glaucoma drainage implant with corneal patch graft in conjunction with keratoprosthesis has a low risk of erosion and postoperative complications.

  1. Spondylolysis outcomes in adolescents after direct screw repair of the pars interarticularis.

    Science.gov (United States)

    Snyder, Laura A; Shufflebarger, Harry; O'Brien, Michael F; Thind, Harjot; Theodore, Nicholas; Kakarla, Udaya K

    2014-09-01

    Isthmic spondylolysis can significantly decrease functional abilities, especially in adolescent athletes. Although treatment can range from observation to surgery, direct screw placement through the fractured pars, or Buck's procedure, may be a more minimally invasive procedure than the more common pedicle screw-hook construct. Review of surgical databases identified 16 consecutive patients treated with Buck's procedure from 2004 to 2010. Twelve patients were treated at Miami Children's Hospital and 4 at Barrow Neurological Institute. Demographics and clinical and radiographic outcomes were recorded and analyzed retrospectively. The 16 patients had a median age of 16 years, and 14 were 20 years or younger at the time of treatment. Symptoms included axial back pain in 100% of patients with concomitant radiculopathy in 38%. Pars defects were bilateral in 81% and unilateral in 19% for a total of 29 pars defects treated using Buck's procedure. Autograft or allograft augmented with recombinant human bone morphogenetic protein as well as postoperative bracing was used in all cases. Postoperatively, symptoms resolved completely or partially in 15 patients (94%). Of 29 pars defects, healing was observed in 26 (89.6%) prior to 1 revision surgery, and an overall fusion rate of 97% was observed at last radiological follow-up. There were no implant failures. All 8 athletes in this group had returned to play at last follow-up. Direct screw repair of the pars interarticularis defect as described in this series may provide a more minimally invasive treatment of adolescent patients with satisfactory clinical and radiological outcomes, including return to play of adolescent athletes.

  2. Astragaloside Alleviates Hepatic Fibrosis Function via PAR2 Signaling Pathway in Diabetic Rats

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    Zhenchang Wang

    2017-02-01

    Full Text Available Background/Aims: Astragaloside (AGS extracted from radix astragalin (Huangqi has been considered to be beneficial to liver diseases. In this study, we examined the role played by AGS in alleviating hepatic fibrosis function via protease-activated receptor-2 (PAR2 mechanisms. We hypothesized that AGS affects PAR2 signaling pathway thereby improving hepatic function in rats with hepatic fibrosis induced by carbon tetrachloride (CCl4. We further hypothesized that AGS attenuates impaired hepatic function evoked by CCl4 to a greater degree in diabetic animals. Methods: ELISA and Western Blot analysis were used to examine PAR2 signaling pathway in diabetic CCl4-rats and non-diabetic CCl4-rats. Results: AGS inhibited the protein expression of PAR2 and its downstream pathway PKA and PKCɛ in CCl4-rats. Notably, the effects of AGS were greater in CCl4-rats with diabetes. AGS also significantly attenuated the CCl4-induced upregulations of pro-inflammatory cytokines, namely interleukin-1β, interleukin-6 and tumor necrosis factor-α accompanied with decreases of collagenic parameters such as hexadecenoic acid, laminin and hydroxyproline. Additionally, AGS improved the CCl4-induced exaggerations of liver index and functions including alanine aminotransferase, aspartate aminotransferase. Moreover, TGF-β1, a marker of hepatic fibrosis, was increased in CCl4-rats and AGS inhibited increases in TGF-β1 induced by CCl4. Conclusions: AGS alleviates hepatic fibrosis by inhibiting PAR2 signaling expression and its effects are largely enhanced in diabetic animals. Targeting one or more of these signaling molecules may present new opportunities for treatment and management of hepatic fibrosis; and results of our study are likely to shed light on strategies for application of AGS because it has potentially greater therapeutic effectiveness for hepatic fibrosis in diabetes.

  3. Synthesis of a polycyclic aromatic hydrocarbon marked with carbon-14: (b, d e f) dibenzo-chrysene {sup 14}C-7,14; Synthese d'un hydrocarbure aromatique polycyclique marque au carbone 14: le dibenzo (b, d e f) chrysene {sup 14}C-7,14

    Energy Technology Data Exchange (ETDEWEB)

    Chatelain, G. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1965-07-01

    (b, d e f) dibenzo-chrysene C-7,14 has been synthesized from radioactive carbon dioxide and the organic magnesium compound derived from 1,5 dibromo naphthalene. The product has been purified by a very precise series of fractionated chromatographs on alumina having a chromatographic activity. This has necessitated the development of a special technique. (author) [French] Le dibenzo (b, d e f) chrysene 14C-7,14 a ete synthetise au depart de gaz carbonique radioactif et de bis-organomagnesien derive du dibromo-1,5 naphtalene. Le produit a ete purifie par une serie de chromatographies fractionnees sur alumine d'activite chromatographique tres precise. Ceci a fait l'objet d'une mise au point de technique. (auteur)

  4. Heavy Element Staining of Sedimentary Organic Matter Functional Groups for Backscattered Electron Imaging Marquage par éléments lourds de la matière organique sédimentaire pour la microscopie électronique en électrons rétrodiffusés

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    Belin-Geindre S.

    2006-11-01

    , 1992. Son imagerie est basée sur les contrastes de numéro atomique (Z existant entre les divers constituants. La matière organique apparaît noire et les minéraux gris. Cependant il n'est pas possible de distinguer plusieurs types de matière organique avec le MEB/ER. Afin de mettre en évidence des contrastes au MEB/ER entre différentes matières organiques, les échantillons ont été marqués par des éléments lourds qui ont la particularité de se fixer de manière sélective et spécifique sur les groupes fonctionnels. Cette méthode est largement employée par la biologie cellulaire pour révéler divers corps organiques (Soligo et Lambertenghi-Deliliers, 1987; Langsdorff et al. , 1990; Clark, 1991; Namimatsu, 1992 ou bien par la science des matériaux dans le domaine des polymères (Sawyer et Grubb, 1987; Ohlsson et Törnell, 1990; Sue et al. , 1991; Janik et al. , 1992. L'application à la géologie et à la pétrographie est restée jusqu'à présent assez réduite : sédiments argileux (Whitlatch et Johnson, 1974; Green et al. , 1979; Bishop et al. , 1992; Hillier et Clayton, 1992, charbons (Corcoran et Stephens, 1987; Sommerfeld et al. , 1992, sols (Foster, 1981. Les éléments lourds utilisés pour le marquage sont très variés et présentent une spécificité plus ou moins large (Reynolds, 1963; Lewis et Knight, 1976; Takeuchi et Takeuchi, 1990. La matière organique sédimentaire est un mélange complexe de macromolécules dont la composition dépend de la nature de la matière organique originelle et de son degré d'évolution (Tissot et Welte, 1984. La matière organique peu évoluée possède de nombreuses variétés de groupes fonctionnels (Rouxhet et al. , 1980; Béhar et Vandenbroucke, 1987. La faisabilité de la méthode a d'abord été testée sur des polymères contenant des fonctions chimiques susceptibles d'exister dans les kérogènes naturels (tableau 1. Puis elle a été appliquée à des sédiments presque exclusivement formés de

  5. Pars plana vitrectomy and silicone oil tamponade for acute endophthalmitis treatment Vitrectomia pars plana e tamponamento por óleo de silicone para o tratamento de endoftalmite aguda

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    Rubens Camargo Siqueira

    2009-02-01

    Full Text Available PURPOSE: To evaluate the outcomes of pars plana vitrectomy and silicone oil injection for the treatment of infectious endophthalmitis. METHODS: 35 cases of endophthalmitis secondary to phacoemulsification (20 patients, trabeculectomy (8 patients, perforating trauma (2 patients, trauma (2 patients, corneal transplantation (1 patient, vitrectomy (1 patient and corneal ulceration (1 patient were retrospectively studied. Patients were separated into two groups: Group 1 (n=24: intravitreal antibiotic injection, associated with topical and oral antibiotics; Group 2 (n=11: vitrectomy with intravitreal antibiotic injection and silicone oil injection. The follow-up ranged from 1 to 48 months (mean of 16 months. RESULTS: From 24 patients in group 1, 11 patients (45.83%, had infection controlled with intravitreal antibiotic injection only; 13 patients (54.15% regressed to uncontrolled endophthalmitis, in which two patients (8.33% were submitted to evisceration and one patient (4.16% had corneal melting. The remaining 10 patients (41.66% with uncontrolled endophthalmitis were submitted to pars plana vitrectomy and silicone oil injection. Six patients (25% from Group I had retinal detachment during the first month of follow-up and also required pars plana vitrectomy and silicone oil injection. In Group 2 patients (n=11, all of them had controlled infection at the first procedure. In one case (9.09%, a severe proliferatative vitreoretinopathy induced loss of vision. CONCLUSION: These results suggest that silicone oil tamponade might be beneficial in the treatment strategy of infectious endophthalmitis.OBJETIVO: Avaliar os resultados da vitrectomia pars plana com tamponamento com óleo de silicone no tratamento de endoftalmite aguda. MÉTODOS: Trinta e cinco pacientes com endoftalmite, sendo 20 secundário à facoemulsificação, 8 por trabeculectomia, 2 por trauma perfurante, 2 por trauma, 1 por transplante de córnea, 1 por vitrectomia, e 1 por úlcera de c

  6. Caracterização de farinhas de tapioca produzidas no estado do Pará Characterization of tapioca flour obtained in Pará state, Brazil

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    Priscilla Andrade Silva

    2012-01-01

    Full Text Available A farinha de tapioca é um alimento produzido artesanalmente a partir da fécula de mandioca (Manihot esculenta Crantz, amplamente consumida na Região Amazônica. O objetivo deste trabalho foi caracterizar duas farinhas de tapioca produzidas no estado do Pará: uma no Baixo Amazonas e outra na Zona Bragantina. As duas farinhas apresentaram perfis granulométricos distintos e diferença significativa (P 0,05 para a maioria dos parâmetros físico-químicos e tecnológicos analisados. A farinha de tapioca proveniente do Baixo Amazonas apresentou maior umidade (10,7%, em função da maior capacidade de adsorver água, devido a sua maior área específica (menor granulometria. A microscopia óptica com luz polarizada, juntamente com as características dos dois produtos indicou a inexistência de um processo padrão utilizado na produção da farinha de tapioca.Tapioca flour is a typical food produced from cassava starch (Manihot esculenta Crantz, widely consumed in the Amazon Region. The aim of this study was to characterize two tapioca flours produced in Pará State, Brazil one in the West Region and other in Bragantina Area. Both tapioca flour presented distinct particle-size distribution and significant difference (P 0.05 for majority of the physico-chemical and technological parameters. Flour from West Region presented high moisture (10.7%, this due the greater capacity of water adsorption, attributed at its high specific area (smaller particle-size. The optical microscopy with polarized light and the technologic properties of the products did not showed a standard process for the production of tapioca flour.

  7. Caracterização de farinhas de tapioca produzidas no estado do Pará Characterization of tapioca flour obtained in Pará state, Brazil

    Directory of Open Access Journals (Sweden)

    Priscilla Andrade Silva

    2013-01-01

    Full Text Available A farinha de tapioca é um alimento produzido artesanalmente a partir da fécula de mandioca (Manihot esculenta Crantz, amplamente consumida na Região Amazônica. O objetivo deste trabalho foi caracterizar duas farinhas de tapioca produzidas no estado do Pará: uma no Baixo Amazonas e outra na Zona Bragantina. As duas farinhas apresentaram perfis granulométricos distintos e diferença significativa (P 0,05 para a maioria dos parâmetros físico-químicos e tecnológicos analisados. A farinha de tapioca proveniente do Baixo Amazonas apresentou maior umidade (10,7%, em função da maior capacidade de adsorver água, devido a sua maior área específica (menor granulometria. A microscopia óptica com luz polarizada, juntamente com as características dos dois produtos indicou a inexistência de um processo padrão utilizado na produção da farinha de tapioca.Tapioca flour is a typical food produced from cassava starch (Manihot esculenta Crantz, widely consumed in the Amazon Region. The aim of this study was to characterize two tapioca flours produced in Pará State, Brazil one in the West Region and other in Bragantina Area. Both tapioca flour presented distinct particle-size distribution and significant difference (P 0.05 for majority of the physico-chemical and technological parameters. Flour from West Region presented high moisture (10.7%, this due the greater capacity of water adsorption, attributed at its high specific area (smaller particle-size. The optical microscopy with polarized light and the technologic properties of the products did not showed a standard process for the production of tapioca flour.

  8. Direct pars repair surgery using two different surgical methods : pedicle screw with universal hook system and direct pars screw fixation in symptomatic lumbar spondylosis patients.

    Science.gov (United States)

    Shin, Myung-Hoon; Ryu, Kyeong-Sik; Rathi, Nitesh Kumar; Park, Chun-Kun

    2012-01-01

    The authors performed a retrospective study to assess the clinical and radiological outcome in symptomatic lumbar spondylolysis patients who underwent a direct pars repair surgery using two different surgical methods; pedicle screw with universal hook system (PSUH) and direct pars screw fixation (DPSF), and compared the results between two different treated groups. Forty-seven consecutive patients (PSUH; 23, DPSF; 15) with symptomatic lumbar spondylolysis who underwent a direct pars repair surgery were included. The average follow-up period was 37 months in the PSUH group, and 28 months in the DPSF group. The clinical outcome was measured using visual analogue pain scale (VAS) and Oswestry disability index (ODI). The length of operation time, the amount of blood loss, the duration of hospital stay, surgical complications, and fusion status were also assessed. When compared to the DPSF group, the average preoperative VAS and ODI score of the PSUH group were less decreased at the last follow-up; (the PSUH group; back VAS : 4.9 vs. 3.0, leg VAS : 6.8 vs. 2.2, ODI : 50.6% vs. 24.6%, the DPSF group; back VAS : 5.7 vs. 1.1, leg VAS : 6.1 vs. 1.2, ODI : 57.4% vs. 18.2%). The average operation time was 174.9 minutes in the PSUH group, and 141.7 minutes in the DPSF group. The average blood loss during operation was 468.8 cc in the PSUH group, and 298.8 cc in the DPSF group. The average hospital stay after operation was 8.9 days in the PSUH group, and 7 days in the DPSF group. In the PSUH group, there was one case of a screw misplacement requiring revision surgery. In the DPSF group, one patient suffered from transient leg pain. The successful bone fusion rate was 78.3% in the PSUH group, and 93.3% in the DPSF group. The present study suggests that the technique using direct pars screw would be more effective than the method using pedicle screw with lamina hook system, in terms of decreased operation time, amount of blood loss, hospital stay, and increased fusion success

  9. Impacts of Light Use Efficiency and fPAR Parameterization on Gross Primary Production Modeling

    Science.gov (United States)

    Cheng, Yen-Ben; Zhang, Qingyuan; Lyapustin, Alexei I.; Wang, Yujie; Middleton, Elizabeth M.

    2014-01-01

    This study examines the impact of parameterization of two variables, light use efficiency (LUE) and the fraction of absorbed photosynthetically active radiation (fPAR or fAPAR), on gross primary production(GPP) modeling. Carbon sequestration by terrestrial plants is a key factor to a comprehensive under-standing of the carbon budget at global scale. In this context, accurate measurements and estimates of GPP will allow us to achieve improved carbon monitoring and to quantitatively assess impacts from cli-mate changes and human activities. Spaceborne remote sensing observations can provide a variety of land surface parameterizations for modeling photosynthetic activities at various spatial and temporal scales. This study utilizes a simple GPP model based on LUE concept and different land surface parameterizations to evaluate the model and monitor GPP. Two maize-soybean rotation fields in Nebraska, USA and the Bartlett Experimental Forest in New Hampshire, USA were selected for study. Tower-based eddy-covariance carbon exchange and PAR measurements were collected from the FLUXNET Synthesis Dataset. For the model parameterization, we utilized different values of LUE and the fPAR derived from various algorithms. We adapted the approach and parameters from the MODIS MOD17 Biome Properties Look-Up Table (BPLUT) to derive LUE. We also used a site-specific analytic approach with tower-based Net Ecosystem Exchange (NEE) and PAR to estimate maximum potential LUE (LUEmax) to derive LUE. For the fPAR parameter, the MODIS MOD15A2 fPAR product was used. We also utilized fAPAR chl, a parameter accounting for the fAPAR linked to the chlorophyll-containing canopy fraction. fAPAR chl was obtained by inversion of a radiative transfer model, which used the MODIS-based reflectances in bands 1-7 produced by Multi-Angle Implementation of Atmospheric Correction (MAIAC) algorithm. fAPAR chl exhibited seasonal dynamics more similar with the flux tower based GPP than MOD15A2 fPAR, especially

  10. Developpement d'algorithmes de reconstruction statistique appliques en tomographie rayons-X assistee par ordinateur

    Science.gov (United States)

    Thibaudeau, Christian

    La tomodensitometrie (TDM) permet d'obtenir, et ce de facon non invasive, une image tridimensionnelle de l'anatomie interne d'un sujet. Elle constitue l'evolution logique de la radiographie et permet l'observation d'un volume sous differents plans (sagittal, coronal, axial ou n'importe quel autre plan). La TDM peut avantageusement completer la tomographie d'emission par positrons (TEP), un outil de predilection utilise en recherche biomedicale et pour le diagnostic du cancer. La TEP fournit une information fonctionnelle, physiologique et metabolique, permettant la localisation et la quantification de radiotraceurs a l'interieur du corps humain. Cette derniere possede une sensibilite inegalee, mais peut neanmoins souffrir d'une faible resolution spatiale et d'un manque de repere anatomique selon le radiotraceur utilise. La combinaison, ou fusion, des images TEP et TDM permet d'obtenir cette localisation anatomique de la distribution du radiotraceur. L'image TDM represente une carte de l'attenuation subie par les rayons-X lors de leur passage a travers les tissus. Elle permet donc aussi d'ameliorer la quantification de l'image TEP en offrant la possibilite de corriger pour l'attenuation. L'image TDM s'obtient par la transformation de profils d'attenuation en une image cartesienne pouvant etre interpretee par l'humain. Si la qualite de cette image est fortement influencee par les performances de l'appareil, elle depend aussi grandement de la capacite de l'algorithme de reconstruction a obtenir une representation fidele du milieu image. Les techniques de reconstruction standards, basees sur la retroprojection filtree (FBP, filtered back-projection), reposent sur un modele mathematiquement parfait de la geometrie d'acquisition. Une alternative a cette methode etalon est appelee reconstruction statistique, ou iterative. Elle permet d'obtenir de meilleurs resultats en presence de bruit ou d'une quantite limitee d'information et peut virtuellement s'adapter a toutes formes

  11. Screening of phytochemicals against protease activated receptor 1 (PAR1), a promising target for cancer.

    Science.gov (United States)

    Kakarala, Kavita Kumari; Jamil, Kaiser

    2015-02-01

    Drug resistance and drug-associated toxicity are the primary causes for withdrawal of many drugs, although patient recovery is satisfactory in many instances. Interestingly, the use of phytochemicals in the treatment of cancer as an alternative to synthetic drugs comes with a host of advantages; minimum side effects, good human absorption and low toxicity to normal cells. Protease activated receptor 1 (PAR1) has been established as a promising target in many diseases including various cancers. Strong evidences suggest its role in metastasis also. There are no natural compounds known to inhibit its activity, so we aimed to identify phytochemicals with antagonist activity against PAR1. We screened phytochemicals from Naturally Occurring Plant-based Anticancer Compound-Activity-Target database (NPACT, http://crdd.osdd.net/raghava/npact/ ) against PAR1 using virtual screening workflow of Schrödinger software. It analyzes pharmaceutically relevant properties using Qikprop and calculates binding energy using Glide at three accuracy levels (high-throughput virtual screening, standard precision and extra precision). Our study led to the identification of phytochemicals, which showed interaction with at least one experimentally determined active site residue of PAR1, showed no violations to Lipinski's rule of five along with predicted high human absorption. Furthermore, structural interaction fingerprint analysis indicated that the residues H255, D256, E260, S344, V257, L258, L262, Y337 and S344 may play an important role in the hydrogen bond interactions of the phytochemicals screened. Of these residues, H255 and L258 residues were experimentally proved to be important for antagonist binding. The residues Y183, L237, L258, L262, F271, L332, L333, Y337, L340, A349, Y350, A352, and Y353 showed maximum hydrophobic interactions with the phytochemicals screened. The results of this work suggest that phytochemicals Reissantins D, 24,25-dihydro-27-desoxywithaferin A, Isoguaiacin

  12. Protease-activated receptor-2 (PAR-2) in the rat gastric mucosa: immunolocalization and facilitation of pepsin/pepsinogen secretion

    Science.gov (United States)

    Kawao, Naoyuki; Sakaguchi, Yuriko; Tagome, Ai; Kuroda, Ryotaro; Nishida, Shozo; Irimajiri, Kiyohiro; Nishikawa, Hiroyuki; Kawai, Kenzo; Hollenberg, Morley D; Kawabata, Atsufumi

    2002-01-01

    Agonists of protease-activated receptor-2 (PAR-2) trigger neurally mediated mucus secretion accompanied by mucosal cytoprotection in the stomach. The present study immunolocalized PAR-2 in the rat gastric mucosa and examined if PAR-2 could modulate pepsin/pepsinogen secretion in rats. PAR-2-like immunoreactivity was abundant in the deep regions of gastric mucosa, especially in chief cells. The PAR-2 agonist SLIGRL-NH2, but not the control peptide LSIGRL-NH2, administered i.v. repeatedly at 0.3 – 1 μmol kg−1, four times in total, significantly facilitated gastric pepsin secretion, although a single dose produced no significant effect. The PAR-2-mediated gastric pepsin secretion was resistant to omeprazole, NG-nitro-L-arginine methyl ester (L-NAME) or atropine, and also to ablation of sensory neurons by capsaicin. Our study thus provides novel evidence that PAR-2 is localized in mucosal chief cells and facilitates gastric pepsin secretion in the rats, most probably by a direct mechanism. PMID:11877338

  13. The role of gyrA and parC mutations in fluoroquinolones-resistant Pseudomonas aeruginosa isolates from Iran

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    Roghayeh Nouri

    Full Text Available Abstract The aim of this study was to examine mutations in the quinolone-resistance-determining region (QRDR of gyrA and parC genes in Pseudomonas aeruginosa isolates. A total of 100 clinical P. aeruginosa isolates were collected from different university-affiliated hospitals in Tabriz, Iran. Minimum inhibitory concentrations (MICs of ciprofloxacin and levofloxacin were evaluated by agar dilution assay. DNA sequences of the QRDR of gyrA and parC were determined by the dideoxy chain termination method. Of the total 100 isolates, 64 were resistant to ciprofloxacin. No amino acid alterations were detected in gyrA or parC genes of the ciprofloxacin susceptible or ciprofloxacin intermediate isolates. Thr-83 → Ile substitution in gyrA was found in all 64 ciprofloxacin resistant isolates. Forty-four (68.75% of them had additional substitution in parC. A correlation was found between the number of the amino acid alterations in the QRDR of gyrA and parC and the level of ciprofloxacin and levofloxacin resistance of the P. aeruginosa isolates. Ala-88 → Pro alteration in parC was generally found in high level ciprofloxacin resistant isolates, which were suggested to be responsible for fluoroquinolone resistance. These findings showed that in P. aeruginosa, gyrA was the primary target for fluoroquinolone and additional mutation in parC led to highly resistant isolates.

  14. RAB-5 controls the cortical organization and dynamics of PAR proteins to maintain C. elegans early embryonic polarity.

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    Vincent Hyenne

    Full Text Available In all organisms, cell polarity is fundamental for most aspects of cell physiology. In many species and cell types, it is controlled by the evolutionarily conserved PAR-3, PAR-6 and aPKC proteins, which are asymmetrically localized at the cell cortex where they define specific domains. While PAR proteins define the antero-posterior axis of the early C. elegans embryo, the mechanism controlling their asymmetric localization is not fully understood. Here we studied the role of endocytic regulators in embryonic polarization and asymmetric division. We found that depleting the early endosome regulator RAB-5 results in polarity-related phenotypes in the early embryo. Using Total Internal Reflection Fluorescence (TIRF microscopy, we observed that PAR-6 is localized at the cell cortex in highly dynamic puncta and depleting RAB-5 decreased PAR-6 cortical dynamics during the polarity maintenance phase. Depletion of RAB-5 also increased PAR-6 association with clathrin heavy chain (CHC-1 and this increase depended on the presence of the GTPase dynamin, an upstream regulator of endocytosis. Interestingly, further analysis indicated that loss of RAB-5 leads to a disorganization of the actin cytoskeleton and that this occurs independently of dynamin activity. Our results indicate that RAB-5 promotes C. elegans embryonic polarity in both dynamin-dependent and -independent manners, by controlling PAR-6 localization and cortical dynamics through the regulation of its association with the cell cortex and the organization of the actin cytoskeleton.

  15. Morphometry, asymmetry and variations of the sylvian fissure and sulci bordering and within the pars triangularis and pars operculum: an autopsy study.

    Science.gov (United States)

    Idowu, Olufemi Emmanuel; Soyemi, Sunday; Atobatele, Kazeem

    2014-11-01

    Speech and Language, one of the most lateralized of all cerebral functions is located within the pars opercularis (PO) and pars triangularis (PT). There is also inter-hemispheric variability of the sulcal contours bordering these areas. The study was undertaken to note the morphometry, asymmetry and variations of the Sylvian fissure (SF), and the sulci bordering and within the PO and PT. An adult autopsy cadaveric study was carried. The measurements made amongst others, included fronto-occipital cerebral length, cerebral width, Sylvian fissure length, and anterior Sylvian point (ASP) to inferior Rolandic point distance. The PT and PO were also studied. Sixty-two adult cadaveric hemispheres were studied. The SF length on the right (mean=84.3mm, median=88mm) was significantly shorter than that on the left (mean=89.4mm, median=92.0mm) (p=0.037). The anterior ascending and anterior horizontal rami of the SF arose from the ASP and either divides at this point (43 hemispheres, 69.4%) or have a common short stem before separating distally giving a Y-shape configuration. The triangularis sulcus was noted in 49 hemispheres (79%) while the diagonal sulcus was noted in 26 hemispheres (41.9%). The left SF was significantly longer than the right and both were positively correlated. The presence of the triangularis sulcus was not dependent on the side (p=0.348) or gender (0.622) unlike the diagonal sulcus was side dependent (p= 0.000).

  16. Linear study and bundle adjustment data fusion; Application to vision localization; Recherche lineaire et fusion de donnees par ajustement de faisceaux; Application a la localisation par vision

    Energy Technology Data Exchange (ETDEWEB)

    Michot, J.

    2010-12-09

    integrate this additional information directly into an extended bundle adjustment, by adding a constraint term in the weighted cost function. We evaluate three methods (based on machine learning or regularization) that dynamically select the weight associated to the constraint and show that these methods can be used in a real time multi-sensor SLAM, and validate them with different types of constraint on the orientation or on the scale. Experiments conducted on real video sequences show that this technique of constrained bundle adjustment reduces the drifts observed with the classical vision algorithms and improves the global accuracy of the positioning system. (author) [French] Les travaux presentes dans ce manuscrit concernent le domaine de la localisation et la reconstruction 3D par vision artificielle. Dans ce contexte, la trajectoire d'une camera et la structure 3D de la scene filmee sont initialement estimees par des algorithmes lineaires puis optimisees par un algorithme non-lineaire, l'ajustement de faisceaux. Cette these presente tout d'abord une technique de recherche de l'amplitude de deplacement (recherche lineaire), ou line search pour les algorithmes de minimisation iterative. La technique proposee est non iterative et peut etre rapidement implantee dans un ajustement de faisceaux traditionnel. Cette technique appelee recherche lineaire algebrique globale (G-ALS), ainsi que sa variante a deux dimensions (Two way-ALS), accelerent la convergence de l'algorithme d'ajustement de faisceaux. L'approximation de l'erreur de reprojection par une distance algebrique rend possible le calcul analytique d'une amplitude de deplacement efficace (ou de deux pour la variante Two way-ALS), par la resolution d'un polynome de degre 3 (G-ALS) ou 5 (Two way-ALS). Nos experimentations sur des donnees simulees et reelles montrent que cette amplitude, optimale en distance algebrique, est performante en distance euclidienne, et permet

  17. Activation of PAR2 receptors sensitizes primary afferents and causes leukocyte rolling and adherence in the rat knee joint

    Science.gov (United States)

    Russell, FA; Schuelert, N; Veldhoen, VE; Hollenberg, MD; McDougall, JJ

    2012-01-01

    Background and Purpose The PAR2 receptors are involved in chronic arthritis by mechanisms that are as yet unclear. Here, we examined PAR2 activation in the rat knee joint. Experimental Approach PAR2 in rat knee joint dorsal root ganglia (DRG) cells at L3-L5, retrogradely labelled with Fluoro-gold (FG) were demonstrated immunohistochemically. Electrophysiological recordings from knee joint nerve fibres in urethane anaesthetized Wistar rats assessed the effects of stimulating joint PAR2 with its activating peptide, 2-furoyl-LIGRLO-NH2 (1–100 nmol·100 μL−1, via close intra-arterial injection). Fibre firing rate was recorded during joint rotations before and 15 min after administration of PAR2 activating peptide or control peptide. Leukocyte kinetics in the synovial vasculature upon PAR2 activation were followed by intravital microscopy for 60 min after perfusion of 2-furoyl-LIGRLO-NH2 or control peptide. Roles for transient receptor potential vanilloid-1 (TRPV1) or neurokinin-1 (NK1) receptors in the PAR2 responses were assessed using the selective antagonists, SB366791 and RP67580 respectively. Key Results PAR2 were expressed in 59 ± 5% of FG-positive DRG cells; 100 nmol 2-furoyl-LIGRLO-NH2 increased joint fibre firing rate during normal and noxious rotation, maximal at 3 min (normal; 110 ± 43%, noxious; 90 ± 31%). 2-Furoyl-LIGRLO-NH2 also significantly increased leukocyte rolling and adhesion over 60 min. All these effects were blocked by pre-treatment with SB366791 and RP67580 (P knee joint via TRPV1- and NK1-dependent mechanisms involving both PAR2-mediated neuronal sensitization and leukocyte trafficking. PMID:22849826

  18. Calculs de champs acoustiques à l'harmonique 2 par décomposition en faisceaux gaussiens

    OpenAIRE

    Vander Meulen, François; Haumesser, Lionel; Fortineau, Jérôme; Bou Matar, Olivier

    2010-01-01

    National audience; Dans le cadre de l'approximation quasi-linéaire, une solution de l'équation KZK peut être obtenue par la méthode des approximations successives qui, dans ce cas,peut être obtenue par la convolution de la fonction source et d'une fonction de Green de forme gaussienne. Wen et Breazeale [1] ont montré que la vitesse particulaire de la surface d'un transducteur axisymétrique pouvait être approchée par une somme de faisceaux gaussiens. Cette décomposition (terme source) permet d...

  19. Rabies in humans and non-human in the state of Pará, Brazilian Amazon

    Directory of Open Access Journals (Sweden)

    Marcus Emanuel Barroncas Fernandes

    Full Text Available We evaluate the relationship of positive cases of rabies with the continuing expansion of livestock production, and analyse the trends of this zoonosis in human population in the state of Pará, Brazilian Amazon. The distribution of rabies cases was recorded between 1999 and 2004. Of 148 cases of rabies, 21% were in humans and 79% in non-human mammals. The rapid growth in livestock numbers seems to be associated with the increase of positive cases in bovine livestock transmitted by vampire bats. This idea is supported by positive and significant relationship of both events in time (p < 0.01, but failed when spatial distribution among regions of the state was considered. However, rabies cases tend to occur toward the northeastern of the state of Pará, where rabies cases are proportionally five times greater than other mesoregions, suggesting that increased livestock production may influence the increase of this zoonosis.

  20. Rabies in humans and non-human in the state of Pará, Brazilian Amazon

    Directory of Open Access Journals (Sweden)

    Marcus Emanuel Barroncas Fernandes

    2013-04-01

    Full Text Available We evaluate the relationship of positive cases of rabies with the continuing expansion of livestock production, and analyse the trends of this zoonosis in human population in the state of Pará, Brazilian Amazon. The distribution of rabies cases was recorded between 1999 and 2004. Of 148 cases of rabies, 21% were in humans and 79% in non-human mammals. The rapid growth in livestock numbers seems to be associated with the increase of positive cases in bovine livestock transmitted by vampire bats. This idea is supported by positive and significant relationship of both events in time (p < 0.01, but failed when spatial distribution among regions of the state was considered. However, rabies cases tend to occur toward the northeastern of the state of Pará, where rabies cases are proportionally five times greater than other mesoregions, suggesting that increased livestock production may influence the increase of this zoonosis.