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Sample records for cycle entry eliminates

  1. Salivary paracetamol elimination kinetics during the menstrual cycle.

    OpenAIRE

    Somaja, L; Thangam, J

    1987-01-01

    Studies were done to examine the influence of the menstrual cycle on the elimination kinetics of paracetamol. Salivary concentrations of paracetamol were determined after oral administration of 1 g of paracetamol on day 3, 10, 14, 20 and 25 of the menstrual cycle in normal healthy women volunteers with regular menstrual cycles. There was no significant difference in elimination half-life (t 1/2) or metabolic clearance rate (CL) between the various days of the menstrual cycle. The result sugge...

  2. Entry into the Postparental Phase of the Family Life Cycle

    Directory of Open Access Journals (Sweden)

    Barbara Wawrzyniak

    2015-02-01

    Full Text Available Der Originalbeitrag in deutscher Sprache ist verfügbar unter: Bd. 40 (2015: Ausgewählte deutsche BeiträgeThe article examines entry into the postparental phase of the family life cycle, which is the familial situation when all children have moved out of the parental household. We position this event chronologically within the life course and examine the probability of occurrence. Using panel data (3 survey waves covering a period of 40 years of a cohort of former North-Rhine Westphalian grammar school pupils, event history models (Cox regression are employed to analyse what factors accelerate or decelerate the transition. This revealed that the parent’s individual biography (in particular the age at the own move out, age at the birth of the first child and the number of children has a major impact on the time of occurrence, while the occupational history has no effect. In addition, sons delay the transition, while children’s academic studies and occupation accelerate it.

  3. Phosphate-Activated Cyclin-Dependent Kinase Stabilizes G1 Cyclin To Trigger Cell Cycle Entry

    Science.gov (United States)

    Menoyo, S.; Ricco, N.; Bru, S.; Hernández-Ortega, S.; Escoté, X.; Aldea, M.

    2013-01-01

    G1 cyclins, in association with a cyclin-dependent kinase (CDK), are universal activators of the transcriptional G1-S machinery during entry into the cell cycle. Regulation of cyclin degradation is crucial for coordinating progression through the cell cycle, but the mechanisms that modulate cyclin stability to control cell cycle entry are still unknown. Here, we show that a lack of phosphate downregulates Cln3 cyclin and leads to G1 arrest in Saccharomyces cerevisiae. The stability of Cln3 protein is diminished in strains with low activity of Pho85, a phosphate-sensing CDK. Cln3 is an in vitro substrate of Pho85, and both proteins interact in vivo. More interestingly, cells that carry a CLN3 allele encoding aspartic acid substitutions at the sites of Pho85 phosphorylation maintain high levels of Cln3 independently of Pho85 activity. Moreover, these cells do not properly arrest in G1 in the absence of phosphate and they die prematurely. Finally, the activity of Pho85 is essential for accumulating Cln3 and for reentering the cell cycle after phosphate refeeding. Taken together, our data indicate that Cln3 is a molecular target of the Pho85 kinase that is required to modulate cell cycle entry in response to environmental changes in nutrient availability. PMID:23339867

  4. Proteomic and protein interaction network analysis of human T lymphocytes during cell-cycle entry

    Science.gov (United States)

    Orr, Stephen J; Boutz, Daniel R; Wang, Rong; Chronis, Constantinos; Lea, Nicholas C; Thayaparan, Thivyan; Hamilton, Emma; Milewicz, Hanna; Blanc, Eric; Mufti, Ghulam J; Marcotte, Edward M; Thomas, N Shaun B

    2012-01-01

    Regulating the transition of cells such as T lymphocytes from quiescence (G0) into an activated, proliferating state involves initiation of cellular programs resulting in entry into the cell cycle (proliferation), the growth cycle (blastogenesis, cell size) and effector (functional) activation. We show the first proteomic analysis of protein interaction networks activated during entry into the first cell cycle from G0. We also provide proof of principle that blastogenesis and proliferation programs are separable in primary human T cells. We employed a proteomic profiling method to identify large-scale changes in chromatin/nuclear matrix-bound and unbound proteins in human T lymphocytes during the transition from G0 into the first cell cycle and mapped them to form functionally annotated, dynamic protein interaction networks. Inhibiting the induction of two proteins involved in two of the most significantly upregulated cellular processes, ribosome biogenesis (eIF6) and hnRNA splicing (SF3B2/SF3B4), showed, respectively, that human T cells can enter the cell cycle without growing in size, or increase in size without entering the cell cycle. PMID:22415777

  5. Targeting of cytosolic phospholipase A2α impedes cell cycle re-entry of quiescent prostate cancer cells.

    Science.gov (United States)

    Yao, Mu; Xie, Chanlu; Kiang, Mei-Yee; Teng, Ying; Harman, David; Tiffen, Jessamy; Wang, Qian; Sved, Paul; Bao, Shisan; Witting, Paul; Holst, Jeff; Dong, Qihan

    2015-10-27

    Cell cycle re-entry of quiescent cancer cells has been proposed to be involved in cancer progression and recurrence. Cytosolic phospholipase A2α (cPLA2α) is an enzyme that hydrolyzes membrane glycerophospholipids to release arachidonic acid and lysophospholipids that are implicated in cancer cell proliferation. The aim of this study was to determine the role of cPLA2α in cell cycle re-entry of quiescent prostate cancer cells. When PC-3 and LNCaP cells were rendered to a quiescent state, the active form of cPLA2α with a phosphorylation at Ser505 was lower compared to their proliferating state. Conversely, the phospho-cPLA2α levels were resurgent during the induction of cell cycle re-entry. Pharmacological inhibition of cPLA2α with Efipladib upon induction of cell cycle re-entry inhibited the re-entry process, as manifested by refrained DNA synthesis, persistent high proportion of cells in G0/G1 and low percentage of cells in S and G2/M phases, together with a stagnant recovery of Ki-67 expression. Simultaneously, Efipladib prohibited the emergence of Skp2 while maintained p27 at a high level in the nuclear compartment during cell cycle re-entry. Inhibition of cPLA2α also prevented an accumulation of cyclin D1/CDK4, cyclin E/CDK2, phospho-pRb, pre-replicative complex proteins CDC6, MCM7, ORC6 and DNA synthesis-related protein PCNA during induction of cell cycle re-entry. Moreover, a pre-treatment of the prostate cancer cells with Efipladib during induction of cell cycle re-entry subsequently compromised their tumorigenic capacity in vivo. Hence, cPLA2α plays an important role in cell cycle re-entry by quiescent prostate cancer cells.

  6. c-Myc activates multiple metabolic networks to generate substrates for cell-cycle entry.

    Energy Technology Data Exchange (ETDEWEB)

    Morrish, Fionnuala M.; Isern, Nancy; Sadilek, Martin; Jeffrey, Mark; Hockenbery, David M.

    2009-05-18

    Cell proliferation requires the coordinated activity of cytosolic and mitochondrial metabolic pathways to provide ATP and building blocks for DNA, RNA, and protein synthesis. Many metabolic pathway genes are targets of the c-myc oncogene and cell cycle regulator. However, the contribution of c-Myc to the activation of cytosolic and mitochondrial metabolic networks during cell cycle entry is unknown. Here, we report the metabolic fates of [U-13C] glucose in serum-stimulated myc-/- and myc+/+ fibroblasts by 13C isotopomer NMR analysis. We demonstrate that endogenous c-myc increased 13C-labeling of ribose sugars, purines, and amino acids, indicating partitioning of glucose carbons into C1/folate and pentose phosphate pathways, and increased tricarboxylic acid cycle turnover at the expense of anaplerotic flux. Myc expression also increased global O-linked GlcNAc protein modification, and inhibition of hexosamine biosynthesis selectively reduced growth of Myc-expressing cells, suggesting its importance in Myc-induced proliferation. These data reveal a central organizing role for the Myc oncogene in the metabolism of cycling cells. The pervasive deregulation of this oncogene in human cancers may be explained by its role in directing metabolic networks required for cell proliferation.

  7. c-Myc activates multiple metabolic networks to generate substrates for cell-cycle entry.

    Science.gov (United States)

    Morrish, F; Isern, N; Sadilek, M; Jeffrey, M; Hockenbery, D M

    2009-07-09

    Cell proliferation requires the coordinated activity of cytosolic and mitochondrial metabolic pathways to provide ATP and building blocks for DNA, RNA and protein synthesis. Many metabolic pathway genes are targets of the c-myc oncogene and cell-cycle regulator. However, the contribution of c-Myc to the activation of cytosolic and mitochondrial metabolic networks during cell-cycle entry is unknown. Here, we report the metabolic fates of [U-(13)C] glucose in serum-stimulated myc(-/-) and myc(+/+) fibroblasts by (13)C isotopomer NMR analysis. We demonstrate that endogenous c-myc increased (13)C labeling of ribose sugars, purines and amino acids, indicating partitioning of glucose carbons into C1/folate and pentose phosphate pathways, and increased tricarboxylic acid cycle turnover at the expense of anaplerotic flux. Myc expression also increased global O-linked N-acetylglucosamine protein modification, and inhibition of hexosamine biosynthesis selectively reduced growth of Myc-expressing cells, suggesting its importance in Myc-induced proliferation. These data reveal a central organizing function for the Myc oncogene in the metabolism of cycling cells. The pervasive deregulation of this oncogene in human cancers may be explained by its function in directing metabolic networks required for cell proliferation.

  8. Influence of seasonal cycles in Martian atmosphere on entry, descent and landing sequence

    Science.gov (United States)

    Marčeta, Dušan; Šegan, Stevo; Rašuo, Boško

    2014-05-01

    The phenomena like high eccentricity of Martian orbit, obliquity of the orbital plane and close alignment of the winter solstice and the orbital perihelion, separately or together can significantly alter not only the level of some Martian atmospheric parameters but also the characteristics of its diurnal and seasonal cycle. Considering that entry, descent and landing (EDL) sequence is mainly driven by the density profile of the atmosphere and aerodynamic characteristic of the entry vehicle. We have performed the analysis of the influence of the seasonal cycles of the atmospheric parameters on EDL profiles by using Mars Global Reference Atmospheric Model (Mars-GRAM). Since the height of the deployment of the parachute and the time passed from the deployment to propulsion firing (descent time) are of crucial importance for safe landing and the achievable landing site elevation we paid special attention to the influence of the areocentric longitude of the Sun (Ls) on these variables. We have found that these variables have periodic variability with respect to Ls and can be very well approximated with a sine wave function whose mean value depends only on the landing site elevation while the amplitudes and phases depend only on the landing site latitude. The amplitudes exhibit behavior which is symmetric with respect to the latitude but the symmetry is shifted from the equator to the northern mid-tropics. We have also noticed that the strong temperature inversions which are usual for middle and higher northern latitudes while Mars is around its orbital perihelion significantly alter the descent time without influencing the height of the parachute deployment. At last, we applied our model to determine the dependence of the accessible landing region on Ls and found that this region reaches maximum when Mars is around the orbital perihelion and can vary 50° in latitude throughout the Martian year.

  9. The ABC-F protein EttA gates ribosome entry into the translation elongation cycle

    Science.gov (United States)

    Boël, Grégory; Smith, Paul C.; Ning, Wei; Englander, Michael T.; Chen, Bo; Hashem, Yaser; Testa, Anthony J.; Fischer, Jeffrey J.; Wieden, Hans-Joachim; Frank, Joachim; Gonzalez, Ruben L.; Hunt, John F.

    2014-01-01

    ABC-F proteins have evaded functional characterization even though they comprise one of the most widely distributed branches of the ATP-binding cassette (ABC) superfamily. Herein, we demonstrate that YjjK, the most prevalent eubacterial ABC-F protein, gates ribosome entry into the translation elongation cycle through a nucleotide-dependent interaction sensitive to ATP/ADP ratio. Accordingly, we rename this protein Energy-dependent Translational Throttle A (EttA). We determined the crystal structure of Escherichia coli EttA and used it to design mutants for biochemical studies, including enzymological assays of the initial steps of protein synthesis. These studies suggest that EttA may regulate protein synthesis in energy-depleted cells, which have a low ATP/ADP ratio. Consistent with this inference, ΔettA cells exhibit a severe fitness defect in long-term stationary phase. These studies demonstrate that an ABC-F protein regulates protein synthesis via a novel mechanism sensitive to cellular energy status. PMID:24389466

  10. Advances in understanding of soil biogeochemical cycles: the mechanism of HS entry into the root interior

    Science.gov (United States)

    Aleksandrova, Olga

    2017-04-01

    Humic substances represent the major reservoir of carbon (C) in ecosystems, and their turnover is crucial for understanding the global C cycle. As shown by some investigators [1-2], the phenomenon of the uptake of the whole humic particles by plant roots is a significant step of biogeochemical cycle of carbon in soils. The mechanism of HS entry the root interior remained unknown for a long time. However recently, the last one was discovered [3]. An advanced model [3] includes two hypotheses. These hypotheses are as follows: (1) each nano-size particle possesses a quantum image that can be revealed as a packet of electromagnetic waves; (2) the interaction of nano-size particle with the membrane (plasma membrane) of living cells, on which it is adsorbed, occurs via the development of the Rayleigh-Taylor (RT) instability on the membrane surface. An advanced model allows us to look insight some into some phenomena that were observed by experiments but remained not understood [2]. The authors [2] applied tritium autoradiography to wheat seedlings cultivated with tritium-labeled HS to consider the uptake of humic particles by plant roots. They found a significant increase in the content of some polar (monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulfoquinovosyl diacylglycerol (SQDG) and phosphatidylcholine (PC)) and neutral (free fatty acids, FFA) lipids which were detected in the wheat seedlings treated with humic particles. Authors [2] pointed that lipids MGDG, DGDG, SQDG are crucial for functional and structural integrity of the photosystem complex. Therefore, a stimulating action of adsorbed humic particles evoked phenomena like photosynthesis in root cells that can be interpreted using an advanced model: humic particles being nano-size particles become adsorbed on the plant roots in soils, and influence their micro environment, where they are located, with the specific electromagnetic exposure. Another finding of authors consisted in the

  11. Cell cycle re-entry mechanisms after DNA damage checkpoints Giving it some gas to shut off the breaks!

    NARCIS (Netherlands)

    van Vugt, Marcel A. T. M.; Yaffe, Michael B.

    2010-01-01

    In order to maintain genetic integrity, cells are equipped with cell cycle checkpoints that detect DNA damage, orchestrate repair, and if necessary, eliminate severely damaged cells by inducing apoptotic cell death. The mitotic machinery is now emerging as an important determinant of the cellular re

  12. Use of Multiple Reheat Helium Brayton Cycles to Eliminate the Intermediate Heat Transfer Loop for Advanced Loop Type SFRs

    Energy Technology Data Exchange (ETDEWEB)

    Haihua Zhao; Hongbin Zhang; Samuel E. Bays

    2009-05-01

    The sodium intermediate heat transfer loop is used in existing sodium cooled fast reactor (SFR) plant design as a necessary safety measure to separate the radioactive primary loop sodium from the water of the steam Rankine power cycle. However, the intermediate heat transfer loop significantly increases the SFR plant cost and decreases the plant reliability due to the relatively high possibility of sodium leakage. A previous study shows that helium Brayton cycles with multiple reheat and intercooling for SFRs with reactor outlet temperature in the range of 510°C to 650°C can achieve thermal efficiencies comparable to or higher than steam cycles or recently proposed supercritical CO2 cycles. Use of inert helium as the power conversion working fluid provides major advantages over steam or CO2 by removing the requirement for safety systems to prevent and mitigate the sodium-water or sodium-CO2 reactions. A helium Brayton cycle power conversion system therefore makes the elimination of the intermediate heat transfer loop possible. This paper presents a pre-conceptual design of multiple reheat helium Brayton cycle for an advanced loop type SFR. This design widely refers the new horizontal shaft distributed PBMR helium power conversion design features. For a loop type SFR with reactor outlet temperature 550°C, the design achieves 42.4% thermal efficiency with favorable power density comparing with high temperature gas cooled reactors.

  13. Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System.

    Science.gov (United States)

    Santaguida, Stefano; Richardson, Amelia; Iyer, Divya Ramalingam; M'Saad, Ons; Zasadil, Lauren; Knouse, Kristin A; Wong, Yao Liang; Rhind, Nicholas; Desai, Arshad; Amon, Angelika

    2017-06-19

    Aneuploidy, a state of karyotype imbalance, is a hallmark of cancer. Changes in chromosome copy number have been proposed to drive disease by modulating the dosage of cancer driver genes and by promoting cancer genome evolution. Given the potential of cells with abnormal karyotypes to become cancerous, do pathways that limit the prevalence of such cells exist? By investigating the immediate consequences of aneuploidy on cell physiology, we identified mechanisms that eliminate aneuploid cells. We find that chromosome mis-segregation leads to further genomic instability that ultimately causes cell-cycle arrest. We further show that cells with complex karyotypes exhibit features of senescence and produce pro-inflammatory signals that promote their clearance by the immune system. We propose that cells with abnormal karyotypes generate a signal for their own elimination that may serve as a means for cancer cell immunosurveillance. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Is the time dimension of the cell cycle re-entry in AD regulated by centromere cohesion dynamics?

    Science.gov (United States)

    Bajić, Vladan P; Spremo-Potparević, Biljana; Zivković, Lada; Djelić, Ninoslav; Smith, Mark A

    2008-01-01

    Chromosomal involvement is a legitimate, yet not well understood, feature of Alzheimer disease (AD). Firstly, AD affects more women than men. Secondly, the amyloid-β protein precursor genetic mutations, responsible for a cohort of familial AD cases, reside on chromosome 21, the same chromosome responsible for the developmental disorder Down's syndrome. Thirdly, lymphocytes from AD patients display a novel chromosomal phenotype, namely premature centromere separation (PCS). Other documented morphological phenomena associated with AD include the occurrence of micronuclei, aneuploidy, binucleation, telomere instability, and cell cycle re-entry protein expression. Based on these events, here we present a novel hypothesis that the time dimension of cell cycle re-entry in AD is highly regulated by centromere cohesion dynamics. In view of the fact that neurons can re-enter the cell division cycle, our hypothesis predicts that alterations in the signaling pathway leading to premature cell death in neurons is a consequence of altered regulation of the separation of centromeres as a function of time. It is well known that centromeres in the metaphase-anaphase transition separate in a non-random, sequential order. This sequence has been shown to be deregulated in aging cells, various tumors, syndromes of chromosome instability, following certain chemical inductions, as well as in AD. Over time, premature chromosome separation is both a result of, and a driving force behind, further cohesion impairment, activation of cyclin dependent kinases, and mitotic catastrophe, a vicious circle resulting in cellular degeneration and death.

  15. Two essays on electricity markets: Entry into hydroelectric generation industry and the political cycle of regulated prices

    Science.gov (United States)

    Moita, Rodrigo Menon Simoes

    This dissertation is about the electricity industry and the problems that arise with the liberalization and de-regulation of the industry. Characteristics intrinsic to the electricity market create problems that can compromise an efficient functioning of this market. Each of the two chapters of this dissertation focus on a specific aspect of this industry. The first chapter analyzes entry in the hydroelectric generation industry. The operation of a generator upstream regularizes the river flow for generators located downstream on the same river, increasing the production capacity of the latter. This positive externality increases the attractiveness of the locations downstream whenever a generator decides to enter upstream. Therefore, the entry decision of a generator in a given location may affect all entry decisions in potential locations for plants located downstream. I first model the problem of generators located in cascade on the same river and show the positive effect of the externality. Second, I use a panel of data on investment decisions of hydro-generation firms to estimate an entry model that takes into account the effect of the externality generated by entry upriver. The results show a positive incentive to locate downstream from existing plants and from locations where entry is likely to occur. Location characteristics also play an important role on the entrants' decisions. The model provides estimates of the average expected market price across the different years covered by the sample and shows that it rose one year before the energy crisis of 2001, evidencing that the market anticipated the crisis. This result has important implications on the evaluation of the Brazilian market design. It shows that entry responded to a rise in expectations about excess demand in the future, contradicting the argument that the crisis was a consequence of mis-designed market institutions. The second chapter deals with the problem of the political cycle in regulated

  16. E2F-dependent induction of p14ARF during cell cycle re-entry in human T cells

    DEFF Research Database (Denmark)

    del Arroyo, Ana Gutierrez; El Messaoudi, Selma; Clark, Paula A

    2007-01-01

    The ARF protein, encoded by alternate exon usage within the CDKN2A locus, provides a link between the retinoblastoma (pRb) and p53 tumor suppressor pathways. Agents that disable pRb or otherwise impinge on the E2F family of transcription factors induce expression of ARF, resulting in stabilization...... of p53 and activation of p53-regulated genes. However, in some cell types ARF is not induced upon cell cycle re-entry, as expected of a conventional E2F target gene, leading to the suggestion that the ARF promoter only responds to supra-physiological or aberrant levels of E2F. These properties have...

  17. Effect of air-entry angle on performance of a 2-stroke-cycle compression-ignition engine

    Science.gov (United States)

    Earle, Sherod L; Dutee, Francis J

    1937-01-01

    An investigation was made to determine the effect of variations in the horizontal and vertical air-entry angles on the performance characteristics of a single-cylinder 2-stroke-cycle compression-ignition test engine. Performance data were obtained over a wide range of engine speed, scavenging pressure, fuel quantity, and injection advance angle with the optimum guide vanes. Friction and blower-power curves are included for calculating the indicated and net performances. The optimum horizontal air-entry angle was found to be 60 degrees from the radial and the optimum vertical angle to be zero, under which conditions a maximum power output of 77 gross brake horsepower for a specific fuel consumption of 0.52 pound per brake horsepower-hour was obtained at 1,800 r.p.m. and 16-1/2 inches of Hg scavenging pressure. The corresponding specific output was 0.65 gross brake horsepower per cubic inch of piston displacement. Tests revealed that the optimum scavenging pressure increased linearly with engine speed. The brake mean effective pressure increased uniformly with air quantity per cycle for any given vane angle and was independent of engine speed and scavenging pressure.

  18. Irreversible APC(Cdh1) Inactivation Underlies the Point of No Return for Cell-Cycle Entry.

    Science.gov (United States)

    Cappell, Steven D; Chung, Mingyu; Jaimovich, Ariel; Spencer, Sabrina L; Meyer, Tobias

    2016-06-30

    Proliferating cells must cross a point of no return before they replicate their DNA and divide. This commitment decision plays a fundamental role in cancer and degenerative diseases and has been proposed to be mediated by phosphorylation of retinoblastoma (Rb) protein. Here, we show that inactivation of the anaphase-promoting complex/cyclosome (APC(Cdh1)) has the necessary characteristics to be the point of no return for cell-cycle entry. Our study shows that APC(Cdh1) inactivation is a rapid, bistable switch initiated shortly before the start of DNA replication by cyclin E/Cdk2 and made irreversible by Emi1. Exposure to stress between Rb phosphorylation and APC(Cdh1) inactivation, but not after APC(Cdh1) inactivation, reverted cells to a mitogen-sensitive quiescent state, from which they can later re-enter the cell cycle. Thus, APC(Cdh1) inactivation is the commitment point when cells lose the ability to return to quiescence and decide to progress through the cell cycle.

  19. ARTD1 regulates cyclin E expression and consequently cell-cycle re-entry and G1/S progression in T24 bladder carcinoma cells.

    Science.gov (United States)

    Léger, Karolin; Hopp, Ann-Katrin; Fey, Monika; Hottiger, Michael O

    2016-08-02

    ADP-ribosylation is involved in a variety of biological processes, many of which are chromatin-dependent and linked to important functions during the cell cycle. However, any study on ADP-ribosylation and the cell cycle faces the problem that synchronization with chemical agents or by serum starvation and subsequent growth factor addition already activates ADP-ribosylation by itself. Here, we investigated the functional contribution of ARTD1 in cell cycle re-entry and G1/S cell cycle progression using T24 urinary bladder carcinoma cells, which synchronously re-enter the cell cycle after splitting without any additional stimuli. In synchronized cells, ARTD1 knockdown, but not inhibition of its enzymatic activity, caused specific down-regulation of cyclin E during cell cycle re-entry and G1/S progression through alterations of the chromatin composition and histone acetylation, but not of other E2F-1 target genes. Although Cdk2 formed a functional complex with the residual cyclin E, p27(Kip 1) protein levels increased in G1 upon ARTD1 knockdown most likely due to inappropriate cyclin E-Cdk2-induced phosphorylation-dependent degradation, leading to decelerated G1/S progression. These results provide evidence that ARTD1 regulates cell cycle re-entry and G1/S progression via cyclin E expression and p27(Kip 1) stability independently of its enzymatic activity, uncovering a novel cell cycle regulatory mechanism.

  20. Guttiferone K impedes cell cycle re-entry of quiescent prostate cancer cells via stabilization of FBXW7 and subsequent c-MYC degradation.

    Science.gov (United States)

    Xi, Z; Yao, M; Li, Y; Xie, C; Holst, J; Liu, T; Cai, S; Lao, Y; Tan, H; Xu, H-X; Dong, Q

    2016-06-02

    Cell cycle re-entry by quiescent cancer cells is an important mechanism for cancer progression. While high levels of c-MYC expression are sufficient for cell cycle re-entry, the modality to block c-MYC expression, and subsequent cell cycle re-entry, is limited. Using reversible quiescence rendered by serum withdrawal or contact inhibition in PTEN(null)/p53(WT) (LNCaP) or PTEN(null)/p53(mut) (PC-3) prostate cancer cells, we have identified a compound that is able to impede cell cycle re-entry through c-MYC. Guttiferone K (GUTK) blocked resumption of DNA synthesis and preserved the cell cycle phase characteristics of quiescent cells after release from the quiescence. In vehicle-treated cells, there was a rapid increase in c-MYC protein levels upon release from the quiescence. However, this increase was inhibited in the presence of GUTK with an associated acceleration in c-MYC protein degradation. The inhibitory effect of GUTK on cell cycle re-entry was significantly reduced in cells overexpressing c-MYC. The protein level of FBXW7, a subunit of E3 ubiquitin ligase responsible for degradation of c-MYC, was reduced upon the release from the quiescence. In contrast, GUTK stabilized FBXW7 protein levels during release from the quiescence. The critical role of FBXW7 was confirmed using siRNA knockdown, which impaired the inhibitory effect of GUTK on c-MYC protein levels and cell cycle re-entry. Administration of GUTK, either in vitro prior to transplantation or in vivo, suppressed the growth of quiescent prostate cancer cell xenografts. Furthermore, elevation of FBXW7 protein levels and reduction of c-MYC protein levels were found in the xenografts of GUTK-treated compared with vehicle-treated mice. Hence, we have identified a compound that is capable of impeding cell cycle re-entry by quiescent PTEN(null)/p53(WT) and PTEN(null)/p53(mut) prostate cancer cells likely by promoting c-MYC protein degradation through stabilization of FBXW7. Its usage as a clinical modality to

  1. Progesterone receptor membrane component-1 (PGRMC1) and PGRMC-2 interact to suppress entry into the cell cycle in spontaneously immortalized rat granulosa cells.

    Science.gov (United States)

    Peluso, John J; Griffin, Daniel; Liu, Xiufang; Horne, Meghan

    2014-11-01

    Progesterone receptor membrane component 1 (PGRMC1) and PGRMC2 are expressed in rat granulosa cells and spontaneously immortalized granulosa cells (SIGCs) but their biological roles are not well defined. The present studies demonstrate that depleting either Pgrmc1 or Pgrmc2 in SIGCs increases entry into the cell cycle but does not increase cell proliferation. Rather, PGRMC1 and/or PGRMC2-deplete cells accumulate in metaphase and undergo apoptosis. Because both PGRMC1 and PGRMC2 localize to the mitotic spindle, their absence likely accounts for cells arresting in metaphase. Moreover, pull-down assays, colocalization studies and in situ proximity ligation assays (PLA) indicate that PGRMC1 binds PGRMC2. Disrupting the PGRMC1:PGRMC2 complex through the use of siRNA or the cytoplasmic delivery of a PGRMC2 antibody increases entry into the cell cycle. Conversely, overexpressing either PGRMC1-GFP or GFP-PGRMC2 fusion protein inhibits entry into the cell cycle. Subsequent studies reveal that depleting PGRMC1 and/or PGRMC2 reduces the percentage of cells in G0 and increases the percentage of cells in G1. These observations indicate that in addition to their role at metaphase, PGRMC1 and PGRMC2 are involved in regulating entry into the G1 stage of the cell cycle. Interestingly, both PGRMC1 and PGRMC2 bind GTPase-activating protein-binding protein 2 (G3BP2) as demonstrated by pull-down assays, colocalization assays, and PLAs. G3bp2 siRNA treatment also promotes entry into the G1 stage. This implies that dynamic changes in the interaction among PGRMC1, PGRMC2, and G3BP2 play an important protein regulating the rate at which SIGCs enter into the cell cycle.

  2. The Hog Cycle of Law Professors: An Econometric Time Series Analysis of the Entry-Level Job Market in Legal Academia.

    Science.gov (United States)

    Engel, Christoph; Hamann, Hanjo

    2016-01-01

    The (German) market for law professors fulfils the conditions for a hog cycle: In the short run, supply cannot be extended or limited; future law professors must be hired soon after they first present themselves, or leave the market; demand is inelastic. Using a comprehensive German dataset, we show that the number of market entries today is negatively correlated with the number of market entries eight years ago. This suggests short-sighted behavior of young scholars at the time when they decide to prepare for the market. Using our statistical model, we make out-of-sample predictions for the German academic market in law until 2020.

  3. Enhanced lithium ion battery cycling of silicon nanowire anodes by template growth to eliminate silicon underlayer islands.

    Science.gov (United States)

    Cho, Jeong-Hyun; Picraux, S Tom

    2013-01-01

    It is well-known that one-dimensional nanostructures reduce pulverization of silicon (Si)-based anode materials during Li ion cycling because they allow lateral relaxation. However, even with improved designs, Si nanowire-based structures still exhibit limited cycling stability for extended numbers of cycles, with the specific capacity retention with cycling not showing significant improvements over commercial carbon-based anode materials. We have found that one important reason for the lack of long cycling stability can be the presence of milli- and microscale Si islands which typically form under nanowire arrays during their growth. Stress buildup in these Si island underlayers with cycling results in cracking, and the loss of specific capacity for Si nanowire anodes, due to progressive loss of contact with current collectors. We show that the formation of these parasitic Si islands for Si nanowires grown directly on metal current collectors can be avoided by growth through anodized aluminum oxide templates containing a high density of sub-100 nm nanopores. Using this template approach we demonstrate significantly enhanced cycling stability for Si nanowire-based lithium-ion battery anodes, with retentions of more than ~1000 mA·h/g discharge capacity over 1100 cycles.

  4. Cyclin Cln3 is retained at the ER and released by the J chaperone Ydj1 in late G1 to trigger cell cycle entry.

    Science.gov (United States)

    Vergés, Emili; Colomina, Neus; Garí, Eloi; Gallego, Carme; Aldea, Martí

    2007-06-08

    G1 cyclin Cln3 plays a key role in linking cell growth and proliferation in budding yeast. It is generally assumed that Cln3, which is present throughout G1, accumulates passively in the nucleus until a threshold is reached to trigger cell cycle entry. We show here that Cln3 is retained bound to the ER in early G1 cells. ER retention requires binding of Cln3 to the cyclin-dependent kinase Cdc28, a fraction of which also associates to the ER. Cln3 contains a chaperone-regulatory Ji domain that counteracts Ydj1, a J chaperone essential for ER release and nuclear accumulation of Cln3 in late G1. Finally, Ydj1 is limiting for release of Cln3 and timely entry into the cell cycle. As protein synthesis and ribosome assembly rates compromise chaperone availability, we hypothesize that Ydj1 transmits growth capacity information to the cell cycle for setting efficient size/ploidy ratios.

  5. Insertional inactivation of the methionine s-methyltransferase gene eliminates the s-methylmethionine cycle and increases the methylation ratio.

    Science.gov (United States)

    Kocsis, Michael G; Ranocha, Philippe; Gage, Douglas A; Simon, Eric S; Rhodes, David; Peel, Gregory J; Mellema, Stefan; Saito, Kazuki; Awazuhara, Motoko; Li, Changjiang; Meeley, Robert B; Tarczynski, Mitchell C; Wagner, Conrad; Hanson, Andrew D

    2003-04-01

    Methionine (Met) S-methyltransferase (MMT) catalyzes the synthesis of S-methyl-Met (SMM) from Met and S-adenosyl-Met (Ado-Met). SMM can be reconverted to Met by donating a methyl group to homocysteine (homo-Cys), and concurrent operation of this reaction and that mediated by MMT sets up the SMM cycle. SMM has been hypothesized to be essential as a methyl donor or as a transport form of sulfur, and the SMM cycle has been hypothesized to guard against depletion of the free Met pool by excess Ado-Met synthesis or to regulate Ado-Met level and hence the Ado-Met to S-adenosylhomo-Cys ratio (the methylation ratio). To test these hypotheses, we isolated insertional mmt mutants of Arabidopsis and maize (Zea mays). Both mutants lacked the capacity to produce SMM and thus had no SMM cycle. They nevertheless grew and reproduced normally, and the seeds of the Arabidopsis mutant had normal sulfur contents. These findings rule out an indispensable role for SMM as a methyl donor or in sulfur transport. The Arabidopsis mutant had significantly higher Ado-Met and lower S-adenosylhomo-Cys levels than the wild type and consequently had a higher methylation ratio (13.8 versus 9.5). Free Met and thiol pools were unaltered in this mutant, although there were moderate decreases (of 30%-60%) in free serine, threonine, proline, and other amino acids. These data indicate that the SMM cycle contributes to regulation of Ado-Met levels rather than preventing depletion of free Met.

  6. Neuronal injury external to the retina rapidly activates retinal glia, followed by elevation of markers for cell cycle re-entry and death in retinal ganglion cells.

    Directory of Open Access Journals (Sweden)

    Alba Galan

    Full Text Available Retinal ganglion cells (RGCs are neurons that relay visual signals from the retina to the brain. The RGC cell bodies reside in the retina and their fibers form the optic nerve. Full transection (axotomy of the optic nerve is an extra-retinal injury model of RGC degeneration. Optic nerve transection permits time-kinetic studies of neurodegenerative mechanisms in neurons and resident glia of the retina, the early events of which are reported here. One day after injury, and before atrophy of RGC cell bodies was apparent, glia had increased levels of phospho-Akt, phospho-S6, and phospho-ERK1/2; however, these signals were not detected in injured RGCs. Three days after injury there were increased levels of phospho-Rb and cyclin A proteins detected in RGCs, whereas these signals were not detected in glia. DNA hyperploidy was also detected in RGCs, indicative of cell cycle re-entry by these post-mitotic neurons. These events culminated in RGC death, which is delayed by pharmacological inhibition of the MAPK/ERK pathway. Our data show that a remote injury to RGC axons rapidly conveys a signal that activates retinal glia, followed by RGC cell cycle re-entry, DNA hyperploidy, and neuronal death that is delayed by preventing glial MAPK/ERK activation. These results demonstrate that complex and variable neuro-glia interactions regulate healthy and injured states in the adult mammalian retina.

  7. Effects of modifications to the space shuttle entry guidance and control systems

    Science.gov (United States)

    Powell, R. W.; Stone, H. W.; Rowell, L. F.

    1976-01-01

    A nonlinear six degree of freedom entry simulation study was conducted to identify space shuttle guidance and control system software modifications which reduce the control system sensitivity to the guidance system sampling frequency. Several modifications which eliminated the control system sensitivity and associated control limit cycling were examined. The result of the modifications was a reduction in required reaction control system fuel.

  8. The Circadian Molecular Clock Regulates Adult Hippocampal Neurogenesis by Controlling the Timing of Cell-Cycle Entry and Exit

    Directory of Open Access Journals (Sweden)

    Pascale Bouchard-Cannon

    2013-11-01

    Full Text Available The subgranular zone (SGZ of the adult hippocampus contains a pool of quiescent neural progenitor cells (QNPs that are capable of entering the cell cycle and producing newborn neurons. The mechanisms that control the timing and extent of adult neurogenesis are not well understood. Here, we show that QNPs of the adult SGZ express molecular-clock components and proliferate in a rhythmic fashion. The clock proteins PERIOD2 and BMAL1 are critical for proper control of neurogenesis. The absence of PERIOD2 abolishes the gating of cell-cycle entrance of QNPs, whereas genetic ablation of bmal1 results in constitutively high levels of proliferation and delayed cell-cycle exit. We use mathematical model simulations to show that these observations may arise from clock-driven expression of a cell-cycle inhibitor that targets the cyclin D/Cdk4-6 complex. Our findings may have broad implications for the circadian clock in timing cell-cycle events of other stem cell populations throughout the body.

  9. The circadian molecular clock regulates adult hippocampal neurogenesis by controlling the timing of cell-cycle entry and exit.

    Science.gov (United States)

    Bouchard-Cannon, Pascale; Mendoza-Viveros, Lucia; Yuen, Andrew; Kærn, Mads; Cheng, Hai-Ying M

    2013-11-27

    The subgranular zone (SGZ) of the adult hippocampus contains a pool of quiescent neural progenitor cells (QNPs) that are capable of entering the cell cycle and producing newborn neurons. The mechanisms that control the timing and extent of adult neurogenesis are not well understood. Here, we show that QNPs of the adult SGZ express molecular-clock components and proliferate in a rhythmic fashion. The clock proteins PERIOD2 and BMAL1 are critical for proper control of neurogenesis. The absence of PERIOD2 abolishes the gating of cell-cycle entrance of QNPs, whereas genetic ablation of bmal1 results in constitutively high levels of proliferation and delayed cell-cycle exit. We use mathematical model simulations to show that these observations may arise from clock-driven expression of a cell-cycle inhibitor that targets the cyclin D/Cdk4-6 complex. Our findings may have broad implications for the circadian clock in timing cell-cycle events of other stem cell populations throughout the body.

  10. Regulation of store-operated Ca{sup 2+} entry activity by cell cycle dependent up-regulation of Orai2 in brain capillary endothelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Kito, Hiroaki [Department of Molecular & Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya (Japan); Department of Pharmacology, Division of Pathological Sciences, Kyoto Pharmaceutical University, Kyoto (Japan); Yamamura, Hisao; Suzuki, Yoshiaki; Yamamura, Hideto [Department of Molecular & Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya (Japan); Ohya, Susumu [Department of Molecular & Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya (Japan); Department of Pharmacology, Division of Pathological Sciences, Kyoto Pharmaceutical University, Kyoto (Japan); Asai, Kiyofumi [Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, Nagoya (Japan); Imaizumi, Yuji, E-mail: yimaizum@phar.nagoya-cu.ac.jp [Department of Molecular & Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya (Japan)

    2015-04-10

    Store-operated Ca{sup 2+} entry (SOCE) via Orai1 and STIM1 complex is supposed to have obligatory roles in the regulation of cellular functions of vascular endothelial cells, while little is known about the contribution of Orai2. Quantitative PCR and Western blot analyses indicated the expression of Orai2 and STIM2, in addition to Orai1 and STIM1 in bovine brain capillary endothelial cell line, t-BBEC117. During the exponential growth of t-BBEC117, the knockdown of Orai1 and STIM1 significantly reduced the SOCE activity, whereas Orai2 and STIM2 siRNAs had no effect. To examine whether endogenous SOCE activity contributes to the regulation of cell cycle progression, t-BBEC117 were synchronized using double thymidine blockage. At the G2/M phase, Ca{sup 2+} influx via SOCE was decreased and Orai2 expression was increased compared to the G0/G1 phase. When Orai2 was knocked down at the G2/M phase, the decrease in SOCE was removed, and cell proliferation was partly attenuated. Taken together, Orai1 significantly contributes to cell proliferation via the functional expression, which is presumably independent of the cell cycle phases. In construct, Orai2 is specifically up-regulated during the G2/M phase, negatively modulates the SOCE activity, and may contribute to the regulation of cell cycle progression in brain capillary endothelial cells. - Highlights: • Orai1 is essential for SOCE activity in brain capillary endothelial cells (BCECs). • Cell cycle independent expression of Orai1 regulated SOCE and cell proliferation. • Orai2 was up-regulated only at G2/M phase and this consequently reduced SOCE. • Orai2 as well as Orai1 is a key player controlling SOCE and proliferation in BCECs.

  11. Palladium Nanoparticles Induce Disturbances in Cell Cycle Entry and Progression of Peripheral Blood Mononuclear Cells: Paramount Role of Ions

    Directory of Open Access Journals (Sweden)

    Claudia Petrarca

    2014-01-01

    Full Text Available There is concern about the possible toxicity of palladium nanoparticles (Pd-NP, as they are released in the environment through many applications. We previously studied the toxicity of Pd-NP at high concentrations; here we address the possible toxicity of Pd-NP at low, subtoxic doses. In particular, we have exposed normal human PBMC entering into the first in vitro mitotic division to Pd-NP and to Pd(IV ions to evaluate ROS generation and cell cycle progression. We have measured a statistically significant increase of intracellular ROS in Pd(IV exposed cells, but not in Pd-NP exposed cells. TEM revealed accumulation of lipid droplets and autophagic and mitophagic vacuoles, which appeared more conspicuous in cells exposed to Pd(IV ions than to Pd-NP. Pd-NP were visible in the cytoplasm of Pd-NP exposed cells. Pd-NP addition was associated with a significant increase of cells within the G0/G1-phase and a significant reduction in GS- and G2/M-phases. Cells exposed to Pd(IV ions showed a significant amplification of these cell cycle alterations. These results suggest that ions, per se or released by NPs, are the true inducers of Pd toxicity. It will be essential to verify whether the observed disturbance represents a temporary response or might result in permanent alterations.

  12. Relationship of cell cycle re-entry and Alzheimer's disease%细胞周期重启与阿尔茨海默病

    Institute of Scientific and Technical Information of China (English)

    李妍; 王海红; 董为人

    2011-01-01

    Alzheimer's disease (AD) is the most common neurodegenerative disease and accounts for more than 60% of the dementia in adults. It is found that terminally differentiated cells upon abnormal stimuli may pass the G0/G1 checkpoint into Gl phase and replicate DNA in S phase and that some cells may even arrive at G2 phase. However they eventually can not enter M phase and complete mitosis. Neurons in the adult central nervous system have classically been described as being in a terminally differentiated state meaning that they no longer return the cell cycle. However, more and more studies show that there is a cell cycle re-entry phenotype in the neurons of AD brain. Cell cycle re-entry may lead to neuron death and is closely related with the pathological changes of AD.%阿尔茨海默病(Alzheimer's disease,AD)是最常见的神经退行性疾病,占成人痴呆症的60%以上.研究表明在受到异常刺激时分化成熟的细胞可以跨过G0/G1检查点进入G1期,并在S期发生DNA复制,有的细胞甚至能够到达G2期,但是最终却不能进入M期完成有丝分裂的整个过程.中枢神经元通常被认为处于终末分化状态,意味着它们不再进入细胞周期.然而,越来越多的研究显示AD患者脑中神经元存在细胞周期的重启,细胞周期重启可导致神经元死亡,细胞周期重启与AD的病理变化关系密切.

  13. TRIIODOTHYRONINE INCREASES MYOCARDIAL FUNCTION AND PYRUVATE ENTRY INTO THE CITRIC ACID CYCLE AFTER REPERFUSION IN A MODEL OF INFANT CARDIOPULMONARY BYPASS

    Energy Technology Data Exchange (ETDEWEB)

    Olson, Aaron; Bouchard, Bertrand; Ning, Xue-Han; Isern, Nancy G.; Des Rosiers, Christine; Portman, Michael A.

    2012-03-01

    We utilized a translational model of infant CPB to test the hypothesis that T3 modulates pyruvate entry into the citric acid cycle (CAC) thereby providing the energy support for improved cardiac function after ischemia-reperfusion. Methods and Results: Neonatal piglets received intracoronary [2-13Carbon(13C)]-pyruvate for 40 minutes (8 mM) during control aerobic conditions (Cont) or immediately after reperfusion (IR) from global hypothermic ischemia. A third group (IR-Tr) received T3 (1.2 ug/kg) during reperfusion. We assessed absolute CAC intermediate levels (aCAC) and flux parameters into the CAC through oxidative pyruvate decarboxylation (PDC ) and anaplerotic carboxylation (PC; ) using 13C-labeled pyruvate and isotopomer analysis by gas and liquid chromatography-mass spectrometry and 13C NMR. Neither IR nor IR-Tr modified aCAC. However, compared to IR, T3 (group IR-Tr) increased cardiac power and oxygen consumption after CPB while elevating both PDC and PC (~ four-fold). T3 inhibited IR induced reductions in CAC intermediate molar percent enrichment (MPE) and oxaloacetate(citrate)/malate MPE ratio; an index of aspartate entry into the CAC. Conclusions: T3 markedly enhances PC and PDC thereby providing substrate for elevated cardiac function and work after reperfusion. The increases in pyruvate flux occur with preservation of the CAC intermediate pool. Additionally, T3 inhibition of reductions in CAC intermediate MPEs indicates that T3 reduces the reliance on amino acids (AA) for anaplerosis after reperfusion. Thus, AA should be more available for other functions such as protein synthesis.

  14. Frailty of two cell cycle checkpoints which prevent entry into mitosis and progression through early mitotic stages in higher plant cells.

    Science.gov (United States)

    del Campo, A; Giménez-Martín, G; López-Sáez, J F; de la Torre, C

    1997-11-01

    Allium cepa L. root meristems were given two short caffeine treatments spaced by 15 hours, the time which roughly corresponds to the duration of one cell cycle. In this way two subsequent cytokineses were prevented, and multinucleate cells with their in complement distributed into two, three or four nuclei were formed. Though all nuclei started to replicate synchronously in these cells, some of them (fast nuclei) completed their replication earlier than others (slow nuclei). The present report shows that two successive checkpoints operate before prometaphase in these cells. The first one prevents the entry of the fast nuclei into prophase until the slow ones have completed their replication. The second checkpoint ensures the synchronous entry into prometaphase after all nuclei have reached and finished prophase. By treating the multinucleate cells with an inhibitor of DNA synthesis at that time when fast but not slow nuclei had finished their replication, it was observed that both checkpoint mechanisms became leaky with time. Under these conditions the fast nuclei entered prophase in the presence of nuclei which were prevented from finishing the replication of their DNA. Subsequently, even prometaphase was triggered after a prolonged prophase. Finally, as expected from the presence of mitotic stages in these cells, nuclei with incompletely replicated DNA endured premature chromosome condensation. The prematurely condensed chromosomes either remained in a prometaphase-like stage until reconstitution nuclei formed or they followed the progression of the fast nuclei into metaphase and anaphase leading to the appearance of acentric chromosomal segments which after reconstitution gave rise to aneuploid nuclei containing unstable and broken DNA.

  15. Triiodothyronine increases myocardial function and pyruvate entry into the citric acid cycle after reperfusion in a model of infant cardiopulmonary bypass.

    Science.gov (United States)

    Olson, Aaron K; Bouchard, Bertrand; Ning, Xue-Han; Isern, Nancy; Rosiers, Christine Des; Portman, Michael A

    2012-03-01

    Triiodothyronine (T3) supplementation improves clinical outcomes in infants after cardiac surgery using cardiopulmonary bypass by unknown mechanisms. We utilized a translational model of infant cardiopulmonary bypass to test the hypothesis that T3 modulates pyruvate entry into the citric acid cycle (CAC), thereby providing the energy support for improved cardiac function after ischemia-reperfusion (I/R). Neonatal piglets received intracoronary [2-(13)Carbon((13)C)]pyruvate for 40 min (8 mM) during control aerobic conditions (control) or immediately after reperfusion (I/R) from global hypothermic ischemia. A third group (I/R-Tr) received T3 (1.2 μg/kg) during reperfusion. We assessed absolute CAC intermediate levels and flux parameters into the CAC through oxidative pyruvate decarboxylation (PDC) and anaplerotic carboxylation (PC) using [2-(13)C]pyruvate and isotopomer analysis by gas and liquid chromatography-mass spectrometry and (13)C-nuclear magnetic resonance spectroscopy. When compared with I/R, T3 (group I/R-Tr) increased cardiac power and oxygen consumption after I/R while elevating flux of both PDC and PC (∼4-fold). Although neither I/R nor I/R-Tr modified absolute CAC levels, T3 inhibited I/R-induced reductions in their molar percent enrichment. Furthermore, (13)C-labeling of CAC intermediates suggests that T3 may decrease entry of unlabeled carbons at the level of oxaloacetate through anaplerosis or exchange reaction with asparate. T3 markedly enhances PC and PDC fluxes, thereby providing potential substrate for elevated cardiac function after reperfusion. This T3-induced increase in pyruvate fluxes occurs with preservation of the CAC intermediate pool. Our labeling data raise the possibility that T3 reduces reliance on amino acids for anaplerosis after reperfusion.

  16. Elimination of the induced malfunctions and use of dynamic prognosis in a thermodynamic diagnosis system of the thermal cycling; Eliminacao das malfuncoes induzidas e utilizacao do prognostico dinamico em sistemas de diagnostico termodinamico de ciclos termicos

    Energy Technology Data Exchange (ETDEWEB)

    Silva, J.A.M.; Venturini, O.J.; Lora, E.E.S. [Universidade Federal de Itajuba (NEST/IEM/UNIFEI), MG (Brazil). Inst. de Engenharia Mecanica. Nucleo de Excelencia em Geracao Termeletrica e Distribuida], Emails: juliomendes@unifei.edu.br, osvaldo@unifei.edu.br, electo@unifei.edu.br

    2009-07-01

    The search for more efficient thermal cycles has led a growing complexity of these cycles and therefore increased the inter-relationships between the thermodynamic components. This complexity is further enhanced with the over time, due to the occurrence of both natural as early degradation in a more or less degree, of all plant components. Several methodologies have been proposed for creating system for diagnostic / prognostic with the objective to detect these degradations (defects) and quantify the gain that can be obtained in the performance indicators of thermal cycles (usually specific fuel consumption and power net) by the elimination of anomalies. Two points have been emphasized as the most difficult in the development of such systems: the distinction between the performance variation of the components due only to the point of operation outside of these project components, caused by the presence of abnormality in another component (induced malfunction) and a decrease in the performance due to the presence of anomalies in the component itself (intrinsic malfunction). The second difficulty is the quantification of the effects induced by the elimination of each of the present anomalies. This work makes use of the method of reconciliation and using individual models of each component to predict unusual behavior of the same design point. The comparison of data from the system plant information, relating to the current state and data from the models, is used to detect anomalies. The methodology is explained in detail and an application of it in a Rankine cycle is used to well understand, especially in parts for the elimination of induced malfunctions, and related to the induced changes by the elimination of each one of the anomalies.

  17. Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis.

    Science.gov (United States)

    Aoi, Yuki; Kawashima, Shigehiro A; Simanis, Viesturs; Yamamoto, Masayuki; Sato, Masamitsu

    2014-07-01

    Analogue-sensitive (as) mutants of kinases are widely used to selectively inhibit a single kinase with few off-target effects. The analogue-sensitive mutant cdc2-as of fission yeast (Schizosaccharomyces pombe) is a powerful tool to study the cell cycle, but the strain displays meiotic defects, and is sensitive to high and low temperature even in the absence of ATP-analogue inhibitors. This has limited the use of the strain for use in these settings. Here, we used in vivo selection for intragenic suppressor mutations of cdc2-as that restore full function in the absence of ATP-analogues. The cdc2-asM17 underwent meiosis and produced viable spores to a similar degree to the wild-type strain. The suppressor mutation also rescued the sensitivity of the cdc2-as strain to high and low temperature, genotoxins and an anti-microtubule drug. We have used cdc2-asM17 to show that Cdc2 activity is required to maintain the activity of the spindle assembly checkpoint. Furthermore, we also demonstrate that maintenance of the Shugoshin Sgo1 at meiotic centromeres does not require Cdc2 activity, whereas localization of the kinase aurora does. The modified cdc2-asM17 allele can be thus used to analyse many aspects of cell-cycle-related events in fission yeast.

  18. US Ports of Entry

    Data.gov (United States)

    Department of Homeland Security — HSIP Non-Crossing Ports-of-Entry A Port of Entry is any designated place at which a CBP officer is authorized to accept entries of merchandise to collect duties, and...

  19. AAV-tau mediates pyramidal neurodegeneration by cell-cycle re-entry without neurofibrillary tangle formation in wild-type mice.

    Directory of Open Access Journals (Sweden)

    Tomasz Jaworski

    Full Text Available In Alzheimer's disease tauopathy is considered secondary to amyloid, and the duality obscures their relation and the definition of their respective contributions.Transgenic mouse models do not resolve this problem conclusively, i.e. the relative hierarchy of amyloid and tau pathology depends on the actual model and the genes expressed or inactivated. Here, we approached the problem in non-transgenic models by intracerebral injection of adeno-associated viral vectors to express protein tau or amyloid precursor protein in the hippocampus in vivo. AAV-APP mutant caused neuronal accumulation of amyloid peptides, and eventually amyloid plaques at 6 months post-injection, but with only marginal hippocampal cell-death. In contrast, AAV-Tau, either wild-type or mutant P301L, provoked dramatic degeneration of pyramidal neurons in CA1/2 and cortex within weeks. Tau-mediated neurodegeneration proceeded without formation of large fibrillar tau-aggregates or tangles, but with increased expression of cell-cycle markers.We present novel AAV-based models, which demonstrate that protein tau mediates pyramidal neurodegeneration in vivo. The data firmly support the unifying hypothesis that post-mitotic neurons are forced to re-enter the cell-cycle in primary and secondary tauopathies, including Alzheimer's disease.

  20. 78 FR 70951 - Agency Information Collection Activities; Proposed Collection; Comment Request; Importer's Entry...

    Science.gov (United States)

    2013-11-27

    ... appropriate, and other forms of information technology. Information Request Regarding Importer's Entry Notice.... This eliminates issues such as ``port shopping'' (attempts to intentionally slip products through one...

  1. Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells

    Energy Technology Data Exchange (ETDEWEB)

    Lauand, C.; Niero, E.L.; Dias, V.M.; Machado-Santelli, G.M. [Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP (Brazil)

    2015-03-06

    Lung cancer often exhibits molecular changes, such as the overexpression of the ErbB1 gene that encodes epidermal growth factor receptor (EGFR). ErbB1 amplification and mutation are associated with tumor aggressiveness and low response to therapy. The aim of the present study was to design a schedule to synchronize the cell cycle of A549 cell line (a non-small cell lung cancer) and to analyze the possible association between the micronuclei (MNs) and the extrusion of ErbB1 gene extra-copies. After double blocking, by the process of fetal bovine serum deprivation and vincristine treatment, MNs formation was monitored with 5-bromo-2-deoxyuridine (BrdU) incorporation, which is an S-phase marker. Statistical analyses allowed us to infer that MNs may arise both in mitosis as well as in interphase. The MNs were able to replicate their DNA and this process seemed to be non-synchronous with the main cell nuclei. The presence of ErbB1 gene in the MNs was evaluated by fluorescent in situ hybridization (FISH). ErbB1 sequences were detected in the MNs, but a relation between the MNs formation and extrusion of amplified ErbB1could not be established. The present study sought to elucidate the meaning of MNs formation and its association with the elimination of oncogenes or other amplified sequences from the tumor cells.

  2. Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells

    Directory of Open Access Journals (Sweden)

    C. Lauand

    2015-05-01

    Full Text Available Lung cancer often exhibits molecular changes, such as the overexpression of the ErbB1 gene that encodes epidermal growth factor receptor (EGFR. ErbB1 amplification and mutation are associated with tumor aggressiveness and low response to therapy. The aim of the present study was to design a schedule to synchronize the cell cycle of A549 cell line (a non-small cell lung cancer and to analyze the possible association between the micronuclei (MNs and the extrusion of ErbB1 gene extra-copies. After double blocking, by the process of fetal bovine serum deprivation and vincristine treatment, MNs formation was monitored with 5-bromo-2-deoxyuridine (BrdU incorporation, which is an S-phase marker. Statistical analyses allowed us to infer that MNs may arise both in mitosis as well as in interphase. The MNs were able to replicate their DNA and this process seemed to be non-synchronous with the main cell nuclei. The presence of ErbB1 gene in the MNs was evaluated by fluorescent in situ hybridization (FISH. ErbB1 sequences were detected in the MNs, but a relation between the MNs formation and extrusion of amplified ErbB1could not be established. The present study sought to elucidate the meaning of MNs formation and its association with the elimination of oncogenes or other amplified sequences from the tumor cells.

  3. Covered Clause Elimination

    CERN Document Server

    Heule, Marijn; Biere, Armin

    2010-01-01

    Generalizing the novel clause elimination procedures developed in [M. Heule, M. J\\"arvisalo, and A. Biere. Clause elimination procedures for CNF formulas. In Proc. LPAR-17, volume 6397 of LNCS, pages 357-371. Springer, 2010.], we introduce explicit (CCE), hidden (HCCE), and asymmetric (ACCE) variants of a procedure that eliminates covered clauses from CNF formulas. We show that these procedures are more effective in reducing CNF formulas than the respective variants of blocked clause elimination, and may hence be interesting as new preprocessing/simplification techniques for SAT solving.

  4. USCIS Backlog Elimination

    Data.gov (United States)

    Department of Homeland Security — USCIS is streamlining the way immigration benefits are delivered. By working smarter and eliminating redundancies, USCIS is bringing a business model to government....

  5. DLMS Voice Data Entry.

    Science.gov (United States)

    1980-06-01

    between operator and computer displayed on ADM-3A 20c A-I Possible Hardware Configuration for a Multistation Cartographic VDES ...this program a Voice Recognition System (VRS) which can be used to explore the use of voice data entry ( VDE ) in the DIMS or other cartographic data...Multi-Station Cartographic Voice Data Entry System An engineering development model voice data entry system ( VDES ) could be most efficiently

  6. Entry at Venus

    Science.gov (United States)

    Venkatapathy, Ethiraj; Smith, Brandon

    2016-01-01

    This is lecture to be given at the IPPW 2016, as part of the 2 day course on Short Course on Destination Venus: Science, Technology and Mission Architectures. The attached presentation material is intended to be introduction to entry aspects of Venus in-situ robotic missions. The presentation introduces the audience to the aerodynamic and aerothermodynamic aspects as well as the loads, both aero and thermal, generated during entry. The course touches upon the system design aspects such as TPS design and both high and low ballistic coefficient entry system concepts that allow the science payload to be protected from the extreme entry environment and yet meet the mission objectives.

  7. Double entry bookkeeping vs single entry bookkeeping

    Directory of Open Access Journals (Sweden)

    Ileana Andreica

    2016-11-01

    Full Text Available Abstract: A financial management eficiently begin, primarily, with an accounting record kept in the best possible conditions, this being conditioned on the adoption of a uniform forms, rational, clear and simple accounting. Throughout history, there have been known two forms of accounting: the simple and double entry. Romanian society after 1990 underwent a substantial change in social structure, the sector on which put a great emphasis being private, that of small manufacturers, peddler, freelance, who work independently and authorized or as associative form (family enterprises, various associations (owners, tenants, etc., liberal professions, etc.. They are obliged to keep a simple bookkeeping, because they have no juridical personality. Companies with legal personality are required to keep double entry bookkeeping; therefore, knowledge and border demarcation between the two forms of organisation of accounting is an essential. The material used for this work is mainly represented by the financial and accounting documents, by the analysis of the economic, by legislative updated sources, and as the method was used the comparison method, using hypothetical data, in case of an authorized individual and a legal entity. Based on the chosen material, an authorized individual (who perform single entry accounting system and a juridical entity (who perform double entry accounting system were selected comparative case studies, using hypothetical data, were analysed advantages and disadvantages in term of fiscal, if using two accounting systems, then were highlighted some conclusion that result.

  8. Methodological aspects of journaling a dynamic adjusting entry model

    Directory of Open Access Journals (Sweden)

    Vlasta Kašparovská

    2011-01-01

    Full Text Available This paper expands the discussion of the importance and function of adjusting entries for loan receivables. Discussion of the cyclical development of adjusting entries, their negative impact on the business cycle and potential solutions has intensified during the financial crisis. These discussions are still ongoing and continue to be relevant to members of the professional public, banking regulators and representatives of international accounting institutions. The objective of this paper is to evaluate a method of journaling dynamic adjusting entries under current accounting law. It also expresses the authors’ opinions on the potential for consistently implementing basic accounting principles in journaling adjusting entries for loan receivables under a dynamic model.

  9. Generating Nice Linear Systems for Matrix Gaussian Elimination

    Science.gov (United States)

    Homewood, L. James

    2004-01-01

    In this article an augmented matrix that represents a system of linear equations is called nice if a sequence of elementary row operations that reduces the matrix to row-echelon form, through matrix Gaussian elimination, does so by restricting all entries to integers in every step. Many instructors wish to use the example of matrix Gaussian…

  10. Minding Rachlin's Eliminative Materialism

    Science.gov (United States)

    McDowell, J. J.

    2012-01-01

    Rachlin's teleological behaviorism eliminates the first-person ontology of conscious experience by identifying mental states with extended patterns of behavior, and thereby maintains the materialist ontology of science. An alternate view, informed by brain-based and externalist philosophies of mind, is shown also to maintain the materialist…

  11. Minding Rachlin's Eliminative Materialism

    Science.gov (United States)

    McDowell, J. J.

    2012-01-01

    Rachlin's teleological behaviorism eliminates the first-person ontology of conscious experience by identifying mental states with extended patterns of behavior, and thereby maintains the materialist ontology of science. An alternate view, informed by brain-based and externalist philosophies of mind, is shown also to maintain the materialist…

  12. Modeling disease elimination.

    Science.gov (United States)

    Somerville, Kevin; Francombe, Paula

    2005-01-01

    The effect of the elimination of mortality from heart disease and cancer was modelled mathematically to allow for the effect of other competing causes of death. The model allows for potential dependence between heart disease or cancer and other causes of death by using cupola functions, which analyse the individual risk itself and the dependence structure between causes of death by using correlation coefficients. As the strength of these risk associations is unknown, the study investigated both full positive and negative dependence and compared this with no dependence. Depending upon the degree and type of correlation assumed, positive or negative, the life expectancy at birth is increased by between 3 months and 6.5 years if cancer mortality was eliminated, and between 5 months and 7.5 years in the case of heart disease. In addition, estimates of these effects on life insurance premia can be made with the greatest reduction for women with the elimination of cancer mortality. These figures provide a range of improvements in life expectancy and the consequent effect on life insurance risk premium rates which elimination of either of these important diseases would produce.

  13. Shuttle entry guidance

    Science.gov (United States)

    Harpold, J. C.; Graves, C. A., Jr.

    1978-01-01

    This paper describes the design of the entry guidance for the Space Shuttle Orbiter. This guidance provides the steering commands for trajectory control from initial penetration of the earth's atmosphere until the terminal area guidance is activated at an earth-relative speed of 2500 fps. At this point, the Orbiter is at a distance of about 50 nmi from the runway threshold, and at an altitude of about 80,000 ft. The entry guidance design is based on an analytic solution of the equations of motion defining the drag acceleration profile that meets the terminal criteria of the entry flight while maintaining the flight within systems and operational constraints. Guidance commands, which are based on a control law that ensures damping of oscillatory type trajectory motion, are computed to steer the Orbiter to this drag acceleration profile.

  14. Eliminating Perinatal HIV Transmission

    Centers for Disease Control (CDC) Podcasts

    2012-11-26

    In this podcast, CDC’s Dr. Steve Nesheim discusses perinatal HIV transmission, including the importance of preventing HIV among women, preconception care, and timely HIV testing of the mother. Dr. Nesheim also introduces the revised curriculum Eliminating Perinatal HIV Transmission intended for faculty of OB/GYN and pediatric residents and nurse midwifery students.  Created: 11/26/2012 by Division of HIV/AIDS Prevention.   Date Released: 11/26/2012.

  15. Targeting rubella for elimination

    Directory of Open Access Journals (Sweden)

    Davendra K Taneja

    2012-01-01

    Full Text Available Rubella is an acute, usually mild viral disease. However, when rubella infection occurs just before conception or during the first 8-10 weeks of gestation, it causes multiple fetal defects in up to 90% of cases, known as Congenital Rubella Syndrome (CRS. It may result in fetal wastage, stillbirths and sensorineural hearing deficit up to 20 weeks of gestation. Rubella vaccine (RA 27/3 is highly effective and has resulted in elimination of rubella and CRS from the western hemisphere and several European countries. Review of several studies documents the duration of protection over 10-21 years following one dose of RA27/3 vaccination, and persistent seropositivity in over 95% cases. Studies in India show seronegativity to rubella among adolescent girls to vary from 10% to 36%. Although due to early age of infection resulting in protection in the reproductive age group, incidence of rubella in India is not very high. However, due to severity of CRS coupled with introduction of RCV in private sector and in some of the states which is likely to lead to sub-optimal coverage and resulting higher risk of rubella during pregnancy in the coming decades, it is imperative to adopt the goal of rubella elimination. As in order to control measles, the country has adopted strategy of delivering second dose of measles through measles campaigns covering children 9 months to 10 years of age in 14 states, it is recommended to synergize efforts for elimination of rubella with these campaigns by replacing measles vaccine by MR or MMR vaccine. Other states which are to give second dose of measles through routine immunization will also have to adopt campaign mode in order to eliminate rubella from the country over 10-20 years. Subsequently, measles vaccine can be replaced by MR or MMR vaccine in the national schedule.

  16. Efforts to Eliminate Illiteracy

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    FOLLOWING the Chinese women's emancipation movement, the Chinese women's illiteracy elimination drive has also gone through a history of 50 years. Although this task is a huge and arduous one, due to various historical, economic and ideological reasons, the rate of illiteracy amongst Chinese women has declined from 90 percent immediately after the founding of New China in 1949 to the present level of 23 percent. The rate of girls going to school has risen from 20 percent to 96.8 percent. Among

  17. Malaria elimination in India and regional implications.

    Science.gov (United States)

    Wangdi, Kinley; Gatton, Michelle L; Kelly, Gerard C; Banwell, Cathy; Dev, Vas; Clements, Archie C A

    2016-10-01

    The malaria situation in India is complex as a result of diverse socio-environmental conditions. India contributes a substantial burden of malaria outside sub-Saharan Africa, with the third highest Plasmodium vivax prevalence in the world. Successful malaria control in India is likely to enhance malaria elimination efforts in the region. Despite modest gains, there are many challenges for malaria elimination in India, including: varied patterns of malaria transmission in different parts of the country demanding area-specific control measures; intense malaria transmission fuelled by favourable climatic and environment factors; varying degrees of insecticide resistance of vectors; antimalarial drug resistance; a weak surveillance system; and poor national coordination of state programmes. Prevention and protection against malaria are low as a result of a weak health-care system, as well as financial and socioeconomic constraints. Additionally, the open borders of India provide a potential route of entry for artesunate-resistant parasites from southeast Asia. This situation calls for urgent dialogue around tackling malaria across borders-between India's states and neighbouring countries-through sharing of information and coordinated control and preventive measures, if we are to achieve the aim of malaria elimination in the region. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Risks: diagnosing and eliminating

    Directory of Open Access Journals (Sweden)

    Yuriy A. Tikhomirov

    2016-01-01

    Full Text Available Objective to develop conceptual theoretical and legal provisions and scientific recommendations on the identification analysis and elimination of risk. Methods universal dialectic method of cognition as well as scientific and private research methods based on it. Results the system was researched of risks diagnostics in the legal sphere and mechanism of influencing the quotrisk situationsquot and their consequences damage to the environment and harm to society. The concept of risk in the legal sphere was formulated the author39s classification of risks in the legal sphere is presented. The rules of analysis evaluation and prevention of risks and the model risk management framework are elaborated. Scientific novelty the mechanism for the identification analysis and elimination of risk has been developed and introduced into scientific circulation the author has proposed the classification and types of risks the reasons and the conditions promoting the risk occurrence. Practical significance the provisions and conclusions of the article can be used in the scientific lawmaking and lawenforcement activity as well as in the educational process of higher educational establishments. nbsp

  19. Deployable Entry-system Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Deployable Entry-system ProjecT (ADEPT) will develop requirements for the ADEPT flight test.  Prior entry systems used high mass thermal protection...

  20. Re-entry and simulation

    Science.gov (United States)

    Jian, H.; Guangming, X.

    1983-11-01

    The physics of re-entry are discussed with emphasis on the re-entry trajectory of a ballistic missile. Factors discussed include re-entry speed, ablation, aerodynamic heating, and the plasma sheath shield. Experimental techniques used to simulate the aerodynamics and performance of missile systems include the computer, the wind tunnel, and free flight. Each of these is briefly discussed.

  1. Using Elimination Theory to construct Rigid Matrices

    CERN Document Server

    Kumar, Abhinav; Patankar, Vijay M; N, Jayalal Sarma M

    2009-01-01

    The rigidity of a matrix A for target rank r is the minimum number of entries of A that must be changed to ensure that the rank of the altered matrix is at most r. Since its introduction by Valiant (1977), rigidity and similar rank-robustness functions of matrices have found numerous applications in circuit complexity, communication complexity, and learning complexity. Almost all nxn matrices over an infinite field have a rigidity of (n-r)^2. It is a long-standing open question to construct infinite families of explicit matrices even with superlinear rigidity when r=Omega(n). In this paper, we construct an infinite family of complex matrices with the largest possible, i.e., (n-r)^2, rigidity. The entries of an nxn matrix in this family are distinct primitive roots of unity of orders roughly exp(n^4 log n). To the best of our knowledge, this is the first family of concrete (but not entirely explicit) matrices having maximal rigidity and a succinct algebraic description. Our construction is based on elimination...

  2. The near elimination of triplets in IVF.

    Science.gov (United States)

    Gerris, Jan

    2007-01-01

    In Antwerp, single embryo transfers (SET) have increased, and this change in policy has seen the incidence of singletons rise from 70 to 90%, twins drop from 25 to 10%, and triplets drop from 1-2% to none at all. At a national and regional level, changes have been much more dramatic as a result of a change in the law in 2003 mandating SET. Data show a huge increase in the number of treatment cycles and a dramatic rise in SET. Rates of twins and triplets have dropped considerably. European data indicate wide differences in the incidence of triplets between countries, varying between 0% (e.g.Slovenia, Iceland, Lithuania) and 4.4% (Hungary), but almost nothing is known about the true incidence of fetal reduction. US data indicate some decrease in triplets during recent years, but certainly nowhere near elimination. These data suggest that in some countries, IVF triplets have almost been eliminated, but the problem is masked by significant fetal reductions. Where SET is not widely used, triplets are still frequent. A correlation appears to exist between the percentage of SET cycles and the incidence of triplets. It is suggested that the solution to almost totally eliminate triplets after IVF is single embryo transfer.

  3. Eliminating rabies in Estonia.

    Directory of Open Access Journals (Sweden)

    Florence Cliquet

    Full Text Available The compulsory vaccination of pets, the recommended vaccination of farm animals in grazing areas and the extermination of stray animals did not succeed in eliminating rabies in Estonia because the virus was maintained in two main wildlife reservoirs, foxes and raccoon dogs. These two species became a priority target therefore in order to control rabies. Supported by the European Community, successive oral vaccination (OV campaigns were conducted twice a year using Rabigen® SAG2 baits, beginning in autumn 2005 in North Estonia. They were then extended to the whole territory from spring 2006. Following the vaccination campaigns, the incidence of rabies cases dramatically decreased, with 266 cases in 2005, 114 in 2006, four in 2007 and three in 2008. Since March 2008, no rabies cases have been detected in Estonia other than three cases reported in summer 2009 and one case in January 2011, all in areas close to the South-Eastern border with Russia. The bait uptake was satisfactory, with tetracycline positivity rates ranging from 85% to 93% in foxes and from 82% to 88% in raccoon dogs. Immunisation rates evaluated by ELISA ranged from 34% to 55% in foxes and from 38% to 55% in raccoon dogs. The rabies situation in Estonia was compared to that of the other two Baltic States, Latvia and Lithuania. Despite regular OV campaigns conducted throughout their territory since 2006, and an improvement in the epidemiological situation, rabies has still not been eradicated in these countries. An analysis of the number of baits distributed and the funding allocated by the European Commission showed that the strategy for rabies control is more cost-effective in Estonia than in Latvia and Lithuania.

  4. Dynamics of Water Entry

    CERN Document Server

    Truscott, Tadd T; Techet, Alexandra H

    2008-01-01

    The hydrodynamics associated with water-entry of spheres can be highly variable with respect to the material and kinematic properties of the sphere. This series of five fluid dynamics videos illustrates several subtle but interesting variations that can be seen. The first series of videos contrasts the nature of impact ($Fr = U_o/\\sqrt{gd} = 5.15$) between a hydrophilic (wetting angle of $\\alpha$ = 60$^\\circ$) and hydrophobic sphere ($\\alpha$ = 120$^\\circ$), and illustrates how surface coating can affect whether or not an air cavity is formed; the views from the side and from above are synchronized in time. The second video series illustrates how spin and surface treatments can alter the splash and cavity formation following water entry. The spinning sphere ($S = \\omega r / U_o = 1.7$; $Fr = 5.15$) causes a wedge of fluid to be drawn into the cavity due to the no-slip condition and follows a curved trajectory. The non-spinning sphere ($Fr = 5.15$) has two distinct surface treatments on the left and right hemi...

  5. Quantum State Detection Via Elimination

    CERN Document Server

    Ettinger, J M; Hoyer, Peter

    1999-01-01

    We present the view of quantum algorithms as a search-theoretic problem. We show that the Fourier transform, used to solve the Abelian hidden subgroup problem, is an example of an efficient elimination observable which eliminates a constant fraction of the candidate secret states with high probability. Finally, we show that elimination observables do not always exist by considering the geometry of the hidden subgroup states of the dihedral group D_N.

  6. 19 CFR 142.17 - One entry summary for multiple entries.

    Science.gov (United States)

    2010-04-01

    ...; DEPARTMENT OF THE TREASURY (CONTINUED) ENTRY PROCESS Entry Summary Documentation § 142.17 One entry summary... for which entries or immediate transportation entries have been filed. However, this provision is not.... (ii) Immediate transportation entries. Immediate transportation entries may be consolidated if the...

  7. Membrane fusion during poxvirus entry.

    Science.gov (United States)

    Moss, Bernard

    2016-12-01

    Poxviruses comprise a large family of enveloped DNA viruses that infect vertebrates and invertebrates. Poxviruses, unlike most DNA viruses, replicate in the cytoplasm and encode enzymes and other proteins that enable entry, gene expression, genome replication, virion assembly and resistance to host defenses. Entry of vaccinia virus, the prototype member of the family, can occur at the plasma membrane or following endocytosis. Whereas many viruses encode one or two proteins for attachment and membrane fusion, vaccinia virus encodes four proteins for attachment and eleven more for membrane fusion and core entry. The entry-fusion proteins are conserved in all poxviruses and form a complex, known as the Entry Fusion Complex (EFC), which is embedded in the membrane of the mature virion. An additional membrane that encloses the mature virion and is discarded prior to entry is present on an extracellular form of the virus. The EFC is held together by multiple interactions that depend on nine of the eleven proteins. The entry process can be divided into attachment, hemifusion and core entry. All eleven EFC proteins are required for core entry and at least eight for hemifusion. To mediate fusion the virus particle is activated by low pH, which removes one or more fusion repressors that interact with EFC components. Additional EFC-interacting fusion repressors insert into cell membranes and prevent secondary infection. The absence of detailed structural information, except for two attachment proteins and one EFC protein, is delaying efforts to determine the fusion mechanism.

  8. Shuttle entry guidance revisited

    Science.gov (United States)

    Mease, Kenneth D.; Kremer, Jean-Paul

    1992-08-01

    The Shuttle entry guidance concept is reviewed which is aimed at tracking a reference drag trajectory that leads to the specified range and velocity for the initiation of the terminal energy management phase. An approximate method of constructing the domain of attraction is proposed, and its validity is ascertained by simulation. An alternative guidance law yielding global exponential tracking in the absence of control saturation is derived using a feedback linearization method. It is noted that the alternative guidance law does not improve on the stability and performance of the current guidance law, for the operating domain and control capability of the Shuttle. It is suggested that the new guidance law with a larger operating domain and increased lift-to-drag capability would be superior.

  9. Shuttle entry guidance revisited

    Science.gov (United States)

    Mease, Kenneth D.; Kremer, Jean-Paul

    1992-01-01

    The Shuttle entry guidance concept is reviewed which is aimed at tracking a reference drag trajectory that leads to the specified range and velocity for the initiation of the terminal energy management phase. An approximate method of constructing the domain of attraction is proposed, and its validity is ascertained by simulation. An alternative guidance law yielding global exponential tracking in the absence of control saturation is derived using a feedback linearization method. It is noted that the alternative guidance law does not improve on the stability and performance of the current guidance law, for the operating domain and control capability of the Shuttle. It is suggested that the new guidance law with a larger operating domain and increased lift-to-drag capability would be superior.

  10. Water Entry of Projectiles

    Science.gov (United States)

    Truscott, Tadd T.; Epps, Brenden P.; Belden, Jesse

    2014-01-01

    The free-surface impact of solid objects has been investigated for well over a century. This canonical problem is influenced by many physical parameters, including projectile geometry, material properties, fluid properties, and impact parameters. Through advances in high-speed imaging and visualization techniques, discoveries about the underlying physics have improved our understanding of these phenomena. Improvements to analytical and numerical models have led to critical insights into cavity formation, the depth and time of pinch-off, forces, and trajectories for myriad different impact parameters. This topic spans a wide range of regimes, from low-speed entry phenomena dominated by surface tension to high-speed ballistics, for which cavitation is important. This review surveys experimental, theoretical, and numerical studies over this broad range, utilizing canonical images where possible to enhance intuition and insight into the rich phenomena.

  11. Principles of safe abdominal entry in laparoscopic gynecologic surgery

    Directory of Open Access Journals (Sweden)

    Jongrak Thepsuwan

    2013-11-01

    Full Text Available Laparoscopic gynecologic surgery has been widely used with a range of benefits. However, there are complications that are related to the abdominal entry process. Serious complications are gastrointestinal tract and major blood vessel injuries. This review introduces the recent available literature to prevent and eliminate the laparoscopic entry complications. The open entry technique is associated with a significant reduction of failed entry, compared to the closed entry technique; however there is no difference in the incidence of visceral or vascular injury. Laparoscopic entry by the left upper abdomen (i.e., Palmer's point or the middle upper abdomen (i.e., the Lee-Huang point could be considered in patients with suspected periumbilical adhesions or a history of umbilical hernia, or after three failed attempts of insufflation at the umbilicus. The Lee-Huang point has its own benefit for the operative laparoscopy in large pelvic pathologies and gynecology malignancy cases. The angle of Veress needle insertion varies from 45° in nonobese women to 90° in extraordinarily obese women. The high intra-peritoneal pressure entries, which range from 20 mmHg to 25 mmHg, minimize the risk of vascular injury. Therefore, this will not adversely affect the cardiopulmonary function in healthy women. The Veress intraperitoneal pressure (<10 mmHg is a reliable indicator of correct intraperitoneal placement of the Veress needle. The elevation of anterior abdominal wall for placement of a Veress needle increases the risks of failed entry and shows no advantage in regard to vascular or visceral complications. Surgeons should continue to increase their knowledge of anatomy, their training, and their experience to decrease laparoscopic complications.

  12. 40 CFR 86.1849-01 - Right of entry.

    Science.gov (United States)

    2010-07-01

    ... Complete Otto-Cycle Heavy-Duty Vehicles § 86.1849-01 Right of entry. (a) Any manufacturer who has applied for certification of a new motor vehicle subject to testing under this subpart, or any manufacturer or... activities connected with such testing are or were performed. (2) Any facility where any new motor vehicle...

  13. Do cooperative cycles of hydrogen bonding exist in proteins?

    CERN Document Server

    Sharley, John N

    2016-01-01

    The closure of cooperative chains of Hydrogen Bonding, HB, to form cycles can enhance cooperativity. Cycles of charge transfer can balance charge into and out of every site, eliminating the charge build-up that limits the cooperativity of open unidirectional chains of cooperativity. If cycles of cooperative HB exist in proteins, these could be expected to be significant in protein structure and function in ways described below. We investigate whether cooperative HB cycles not traversing solvent, ligand or modified residues occur in protein by means including search of Nuclear Magnetic Resonance spectroscopy entries of the Protein Data Bank. We find no mention of an example of this kind of cycle in the literature. For amide-amide HB, for direct inter-amide interactions, when the energy associated with Natural Bond Orbital, NBO, steric exchange is deducted from that of NBO donor-acceptor interactions, the result is close to zero, so that HB is not primarily due to the sum of direct inter-amide NBO interactions....

  14. Toward the Elimination of Paper Orders

    Science.gov (United States)

    Ramirez, Ricardo; Webster, S. Luke

    2016-01-01

    Summary With the adoption of Computerized Patient Order Entry (CPOE), many physicians – particularly consultants and those who are affiliated with multiple hospital systems – are faced with the challenge of learning to navigate and commit to memory the details of multiple EHRs and CPOE software modules. These physicians may resist CPOE adoption, and their refusal to use CPOE presents a risk to patient safety when paper and electronic orders co-exist, as paper orders generated in an electronic ordering environment can be missed or acted upon after delay, are frequently illegible, and bypass the Clinical Decision Support (CDS) that is part of the evidence-based value of CPOE. We defined a category of CPOE Low Frequency Users (LFUs) – physicians issuing a total of less than 10 orders per month – and found that 50.4% of all physicians issuing orders in 3 urban/suburban hospitals were LFUs and actively issuing orders across all shifts and days of the week. Data are presented for 2013 on the number of LFUs by month, day of week, shift and facility, over 2.3 million orders issued. A menu of 6 options to assist LFUs in the use of CPOE, from which hospital leaders could select, was instituted so that paper orders could be increasingly eliminated. The options, along with their cost implications, are described, as is the initial option selected by hospital leaders. In practice, however, a mixed pattern involving several LFU support options emerged. We review data on how the option mix selected may have impacted CPOE adoption and physician use rates at the facilities. The challenge of engaging LFU physicians in CPOE adoption may be common in moderately sized hospitals, and these options can be deployed by other systems in advancing CPOE pervasiveness of use and the eventual elimination of paper orders. PMID:27081405

  15. Tactile Data Entry System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Building on our successful Phase I Tactile Data Entry program, Barron Associates proposes development of a Glove-Enabled Computer Operations (GECO) system to permit...

  16. Entry, Descent, Landing Animation (Animation)

    Science.gov (United States)

    2005-01-01

    [figure removed for brevity, see original site] Click on the image for Entry, Descent, Landing animation This animation illustrates the path the Stardust return capsule will follow once it enters Earth's atmosphere.

  17. Entry, Descent, Landing Animation (Animation)

    Science.gov (United States)

    2005-01-01

    [figure removed for brevity, see original site] Click on the image for Entry, Descent, Landing animation This animation illustrates the path the Stardust return capsule will follow once it enters Earth's atmosphere.

  18. Orion Entry Handling Qualities Assessments

    Science.gov (United States)

    Bihari, B.; Tiggers, M.; Strahan, A.; Gonzalez, R.; Sullivan, K.; Stephens, J. P.; Hart, J.; Law, H., III; Bilimoria, K.; Bailey, R.

    2011-01-01

    The Orion Command Module (CM) is a capsule designed to bring crew back from the International Space Station (ISS), the moon and beyond. The atmospheric entry portion of the flight is deigned to be flown in autopilot mode for nominal situations. However, there exists the possibility for the crew to take over manual control in off-nominal situations. In these instances, the spacecraft must meet specific handling qualities criteria. To address these criteria two separate assessments of the Orion CM s entry Handling Qualities (HQ) were conducted at NASA s Johnson Space Center (JSC) using the Cooper-Harper scale (Cooper & Harper, 1969). These assessments were conducted in the summers of 2008 and 2010 using the Advanced NASA Technology Architecture for Exploration Studies (ANTARES) six degree of freedom, high fidelity Guidance, Navigation, and Control (GN&C) simulation. This paper will address the specifics of the handling qualities criteria, the vehicle configuration, the scenarios flown, the simulation background and setup, crew interfaces and displays, piloting techniques, ratings and crew comments, pre- and post-fight briefings, lessons learned and changes made to improve the overall system performance. The data collection tools, methods, data reduction and output reports will also be discussed. The objective of the 2008 entry HQ assessment was to evaluate the handling qualities of the CM during a lunar skip return. A lunar skip entry case was selected because it was considered the most demanding of all bank control scenarios. Even though skip entry is not planned to be flown manually, it was hypothesized that if a pilot could fly the harder skip entry case, then they could also fly a simpler loads managed or ballistic (constant bank rate command) entry scenario. In addition, with the evaluation set-up of multiple tasks within the entry case, handling qualities ratings collected in the evaluation could be used to assess other scenarios such as the constant bank angle

  19. Currency union entries and trade

    OpenAIRE

    Nitsch, Volker

    2005-01-01

    Recent research suggests that adopting a common currency increases bilateral trade. In this paper, I explore experiences of currency union entry in the post-war period and find no effect on trade. Previous results derived from a large panel data set (covering more than 200 countries from 1948 through 1997) appear to depend crucially on the assumption of symmetry between currency union exits and entries: While countries leaving a currency union experience significant declines in trade, currenc...

  20. Microcystin elimination during sediment contact.

    Science.gov (United States)

    Grützmacher, Gesche; Wessel, Gabriele; Klitzke, Sondra; Chorus, Ingrid

    2010-01-15

    Microcystins (MCYSTs) are a group of structurally similar toxic peptides produced by cyanobacteria ("blue-green algae") which occur frequently in surface waters worldwide. Reliable elimination is necessary when using these waters as drinking water sources. Bank filtration and artificial groundwater recharge utilize adsorption and degradation processes in the subsurface, commonly through sand and gravel aquifers, for the elimination of a wide range of substances during drinking water (pre-) treatment. To obtain parameters for estimating whether MCYST breakthrough is likely in field settings, we tested MCYST elimination in laboratory experiments (batch experiments, column experiments) under a range of conditions. Adsorption coefficients (k(d)-values) obtained from batch studies ranged from 0.2 mL/g for filter sand to 11.6 mL/g for fine grained aquifer materials with 2% fine grains (<63 microm) and 0.8% organic matter. First order degradation rates in column studies reached 1.87 d(-1) under aerobic conditions and showed high variations under anoxic conditions (<0.01-1.35 d(-1)). These results show that, next to sediment texture, redox conditions play an important role for MCYST elimination during sediment passage. Biodegradation was identified as the dominating process for MCYST elimination in sandy aquifer material.

  1. Features of Human Herpesvirus-6A and -6B Entry

    Directory of Open Access Journals (Sweden)

    Takahiro Maeki

    2012-01-01

    Full Text Available Human herpesvirus-6 (HHV-6 is a T lymphotropic herpesvirus belonging to the Betaherpesvirinae subfamily. HHV-6 was long classified into variants A and B (HHV-6A and HHV-6B; however, recently, HHV-6A and HHV-6B were reclassified as different species. The process of herpesvirus entry into target cells is complicated, and in the case of HHV-6A and HHV-6B, the detailed mechanism remains to be elucidated, although both viruses are known to enter cells via endocytosis. In this paper, (1 findings about the cellular receptor and its ligand for HHV-6A and HHV-6B are summarized, and (2 a schematic model of HHV-6A’s replication cycle, including its entry, is presented. In addition, (3 reports showing the importance of lipids in both the HHV-6A envelope and target-cell membrane for viral entry are reviewed, and (4 glycoproteins involved in cell fusion are discussed.

  2. The selective elimination of messenger RNA underlies the mitosis-meiosis switch in fission yeast.

    Science.gov (United States)

    Yamamoto, Masayuki

    2010-01-01

    The cellular programs for meiosis and mitosis must be strictly distinguished but the mechanisms controlling the entry to meiosis remain largely elusive in higher organisms. In contrast, recent analyses in yeast have shed new light on the mechanisms underlying the mitosis-meiosis switch. In this review, the current understanding of these mechanisms in the fission yeast Schizosaccharomyces pombe is discussed. Meiosis-inducing signals in this microbe emanating from environmental conditions including the nutrient status converge on the activity of an RRM-type RNA-binding protein, Mei2. This protein plays pivotal roles in both the induction and progression of meiosis and has now been found to govern the meiotic program in a quite unexpected manner. Fission yeast contains an RNA degradation system that selectively eliminates meiosis-specific mRNAs during the mitotic cell cycle. Mmi1, a novel RNA-binding protein of the YTH-family, is essential for this process. Mei2 tethers Mmi1 and thereby stabilizes the transcripts necessary for the progression of meiosis.

  3. 19 CFR 191.143 - Drawback entry.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) DRAWBACK Foreign-Built Jet Aircraft Engines Processed in the United States § 191.143 Drawback entry. (a) Filing of entry. Drawback entries covering these foreign-built jet aircraft engines shall be filed on Customs Form 7551, modified to show that the entry covers jet aircraft engines processed...

  4. 19 CFR 142.16 - Entry summary documentation.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry summary documentation. 142.16 Section 142.16... TREASURY (CONTINUED) ENTRY PROCESS Entry Summary Documentation § 142.16 Entry summary documentation. (a) Entry summary not filed at time of entry. When the entry documentation is filed before the entry...

  5. Autonomous gliding entry guidance with

    Directory of Open Access Journals (Sweden)

    Guo Jie

    2015-10-01

    Full Text Available This paper presents a novel three-dimensional autonomous entry guidance for relatively high lift-to-drag ratio vehicles satisfying geographic constraints and other path constraints. The guidance is composed of onboard trajectory planning and robust trajectory tracking. For trajectory planning, a longitudinal sub-planner is introduced to generate a feasible drag-versus-energy profile by using the interpolation between upper boundary and lower boundary of entry corridor to get the desired trajectory length. The associated magnitude of the bank angle can be specified by drag profile, while the sign of bank angle is determined by lateral sub-planner. Two-reverse mode is utilized to satisfy waypoint constraints and dynamic heading error corridor is utilized to satisfy no-fly zone constraints. The longitudinal and lateral sub-planners are iteratively employed until all of the path constraints are satisfied. For trajectory tracking, a novel tracking law based on the active disturbance rejection control is introduced. Finally, adaptability tests and Monte Carlo simulations of the entry guidance approach are performed. Results show that the proposed entry guidance approach can adapt to different entry missions and is able to make the vehicle reach the prescribed target point precisely in spite of geographic constraints.

  6. Reconstruction of the Genesis Entry

    Science.gov (United States)

    Desai, Prasun N.; Qualls, Garry D.; Schoenenberger, Mark

    2007-01-01

    An overview of the reconstruction analyses performed for the Genesis capsule entry is described. The results indicate that the actual entry prior to the drogue deployment failure was very close to the pre-entry predictions. The capsule landed 8.3 km south of the desired target at Utah Test and Training Range. Analysis on infrared video footage (obtained from the tracking stations) during the descent estimated the onset of the capsule tumble at Mach 0.9. Frequency analysis on the infrared video data indicates that the aerodynamics generated for the Genesis capsule reasonably predicted the drag and static stability. Observations of the heatshield support the pre-entry simulation estimates of a small hypersonic angles-of-attack, since there is very little, if any, charring of the shoulder region or the aftbody. Through this investigation, an overall assertion can be made that all the data gathered from the Genesis entry is consistent with flight performance that was close to the nominal preentry prediction. Consequently, the design principles and methodologies utilized for the flight dynamics, aerodynamics, and aerothermodynamics analyses have been corroborated.

  7. ZPC Matrices and Zero Cycles

    Directory of Open Access Journals (Sweden)

    Marina Arav

    2009-01-01

    Full Text Available Let H be an m×n real matrix and let Zi be the set of column indices of the zero entries of row i of H. Then the conditions |Zk∩(∪i=1k−1Zi|≤1 for all k  (2≤k≤m are called the (row Zero Position Conditions (ZPCs. If H satisfies the ZPC, then H is said to be a (row ZPC matrix. If HT satisfies the ZPC, then H is said to be a column ZPC matrix. The real matrix H is said to have a zero cycle if H has a sequence of at least four zero entries of the form hi1j1,hi1j2,hi2j2,hi2j3,…,hikjk,hikj1 in which the consecutive entries alternatively share the same row or column index (but not both, and the last entry has one common index with the first entry. Several connections between the ZPC and the nonexistence of zero cycles are established. In particular, it is proved that a matrix H has no zero cycle if and only if there are permutation matrices P and Q such that PHQ is a row ZPC matrix and a column ZPC matrix.

  8. Membongkar Akuntansi Double Entry Systems

    Directory of Open Access Journals (Sweden)

    Whedy Prasetyo

    2013-08-01

    Full Text Available Double entry isa process of recording transactions that always involve at least two accounts ofwhich changes (increases or decreases have consequences to maintain a balance equation. This is a simple basic accounting, that the use of the funds must alwaysbe equal to the acquisition of funds. The equation is not able to include some business events that are responses to the development of business environment. A proposed view is based on the interaction of researcher (New Bond with accountant (Mrs M to dismantle the double entry accounting through Maurice Merleau Ponty's approach (1906-1961. The results prove that physical aspects of knowledge willcontinue to evolve according to environmental interactions.

  9. The General Technique of Selected Harmonics Elimination for Multilevel Converters

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    For high power applications, multilevel converters have many advantages in comparison with other circuit topologies with output transformers. Cascaded inverters are one type of multilevel converters, they are easy to implement, very suitable for modularized layout and packaging. Their manufacturing cost is low. A multilevel PWM technique, called as General Technique of Selected Harmonics Elimination(GTSHE), is proposed in the paper. A general harmonic elimination equation for N cells, M pulses per half cycle, nth harmonic is derived, and verified by simulation results.

  10. Methane elimination methods on landfills

    OpenAIRE

    Ponikvar, Martin

    2008-01-01

    Increase in substance and energy exploitation from waste which reduces the greenhouse gas emissions are one of the main goals of environmental protection. Slovenian legislation defines that in case captured landfill gas cannot be used for energy acquisition, incineration on the landfill itself or some other equivalent procedure must be used to prevent gas emissions into the air. The graduation thesis is based on existing municipal landfill Volče where suitable gas elimination is yet to be arr...

  11. Method for eliminating artifacts in CCD imagers

    Science.gov (United States)

    Turko, B. T.; Yates, G. J.

    1990-06-01

    An electronic method for eliminating artifacts in a video camera employing a charge coupled device (CCD) as an image sensor is presented. The method comprises the step of initializing the camera prior to normal readout. The method includes a first dump cycle period for transferring radiation generated charge into the horizontal register. This occurs while the decaying image on the phosphor being imaged is being integrated in the photosites, and a second dump cycle period, occurring after the phosphor image has decayed, for rapidly dumping unwanted smear charge which has been generated in the vertical registers. Image charge is then transferred from the photosites and to the vertical registers and readout in conventional fashion. The inventive method allows the video camera to be used in environments having high ionizing radiation content, and to capture images of events of very short duration and occurring either within or outside the normal visual wavelength spectrum. Resultant images are free from ghost, smear, and smear phenomena caused by insufficient opacity of the registers, and are also free from random damage caused by ionization charges which exceed the charge limit capacity of the photosites.

  12. Lunar periodicity and the timing of river entry in Atlantic salmon Salmo salar.

    Science.gov (United States)

    Kuparinen, A; O'Hara, R B; Merilä, J

    2009-07-01

    Historical catch records of Atlantic salmon Salmo salar from three rivers discharging to the Baltic Sea in an area free from tides and from strong effects of the moon on illumination were analysed to investigate whether timing of S. salar river entry was associated with lunar cycles directly. Although a significant effect of lunar phase on river entry was detected, with more fish entering rivers around the full moon than other phases, the effect of the lunar cycle was very small compared with other sources of variation. Hence, the biological role of lunar cycle as a determinant of the timing of S. salar runs in the investigated populations was negligible, suggesting that lunar cycle per se does not play a role in the timing of S. salar river entry.

  13. Re-entry flight clearance

    NARCIS (Netherlands)

    Juliana, S.

    2006-01-01

    The objective of the research was to identify and evaluate promising mathematical techniques for re-entry flight clearance. To fulfil this objective, two mathematical methods were investigated and developed: μ analysis for linear models and interval analysis for both linear and non-linear model

  14. Components of Visual Prior Entry

    Science.gov (United States)

    Schneider, Keith A.; Bavelier, Daphne

    2003-01-01

    The prior entry hypothesis contends that attention accelerates sensory processing, shortening the time to perception. Typical observations supporting the hypothesis may be explained equally well by response biases, changes in decision criteria, or sensory facilitation. In a series of experiments conducted to discriminate among the potential…

  15. Entry Facilitation by Environmental Groups

    NARCIS (Netherlands)

    van der Made, Allard; Schoonbeek, Lambert

    We consider a model of vertical product differentiation where consumers care about the environmental damage their consumption causes. An environmental group is capable of increasing consumers' environmental concern via a costly campaign. We show that the prospect of such a campaign can induce entry

  16. Entry Facilitation by Environmental Groups

    NARCIS (Netherlands)

    van der Made, Allard; Schoonbeek, Lambert

    2009-01-01

    We consider a model of vertical product differentiation where consumers care about the environmental damage their consumption causes. An environmental group is capable of increasing consumers' environmental concern via a costly campaign. We show that the prospect of such a campaign can induce entry

  17. 19 CFR 142.3a - Entry numbers.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry numbers. 142.3a Section 142.3a Customs... (CONTINUED) ENTRY PROCESS Entry Documentation § 142.3a Entry numbers. (a) Placement on CBP forms. The importer or broker shall place an 11 character entry number on the entry and corresponding entry...

  18. Bi-weekly waterfowl survey data entry

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Data sheet for the entry of bi-weekly waterfowl survey data from the state of Kansas. This Excel file contains the data entry sheet and a chart displaying waterfowl...

  19. Flavivirus Entry Receptors: An Update

    Directory of Open Access Journals (Sweden)

    Manuel Perera-Lecoin

    2013-12-01

    Full Text Available Flaviviruses enter host cells by endocytosis initiated when the virus particles interact with cell surface receptors. The current model suggests that flaviviruses use at least two different sets of molecules for infectious entry: attachment factors that concentrate and/or recruit viruses on the cell surface and primary receptor(s that bind to virions and direct them to the endocytic pathway. Here, we present the currently available knowledge regarding the flavivirus receptors described so far with specific attention to C-type lectin receptors and the phosphatidylserine receptors, T-cell immunoglobulin and mucin domain (TIM and TYRO3, AXL and MER (TAM. Their role in flavivirus attachment and entry as well as their implication in the virus biology will be discussed in depth.

  20. 19 CFR 122.42 - Aircraft entry.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Aircraft entry. 122.42 Section 122.42 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Aircraft Entry and Entry Documents; Electronic Manifest Requirements...

  1. Sunk costs, entry deterrence, and financial constraints

    NARCIS (Netherlands)

    Arping, S.; Diaw, K.M.

    2008-01-01

    This paper studies how sunk costs affect a financially constrained incumbent's ability to deter entry into its market. Sunk costs make it less attractive to the incumbent to accommodate entry by liquidating assets in place and exiting the market. This may render entry by a prospective rival

  2. Sport entries and qualification manual: Nanjing 2014

    OpenAIRE

    2014-01-01

    Sport entries constitute an important part of the delegation registration process of the Nanjing 2014 Summer Youth Olympic Games. This manual aims to introduce to NOCs the process, relevant policies and requirements regarding sport entries so as to ensure that NOCs can successfully complete the entries for their athletes to the Nanjing 2014 Youth Olympic Games.

  3. 19 CFR 143.36 - Form of immediate delivery, entry and entry summary.

    Science.gov (United States)

    2010-04-01

    ... Form of immediate delivery, entry and entry summary. (a) Electronic form of data. If Customs determines... 19 Customs Duties 2 2010-04-01 2010-04-01 false Form of immediate delivery, entry and entry summary. 143.36 Section 143.36 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF...

  4. Increasing the potential for malaria elimination by targeting zoophilic vectors

    Science.gov (United States)

    Waite, Jessica L.; Swain, Sunita; Lynch, Penelope A.; Sharma, S. K.; Haque, Mohammed Asrarul; Montgomery, Jacqui; Thomas, Matthew B.

    2017-01-01

    Countries in the Asia Pacific region aim to eliminate malaria by 2030. A cornerstone of malaria elimination is the effective management of Anopheles mosquito vectors. Current control tools such as insecticide treated nets or indoor residual sprays target mosquitoes in human dwellings. We find in a high transmission region in India, malaria vector populations show a high propensity to feed on livestock (cattle) and rest in outdoor structures such as cattle shelters. We also find evidence for a shift in vector species complex towards increased zoophilic behavior in recent years. Using a malaria transmission model we demonstrate that in such regions dominated by zoophilic vectors, existing vector control tactics will be insufficient to achieve elimination, even if maximized. However, by increasing mortality in the zoophilic cycle, the elimination threshold can be reached. Current national vector control policy in India restricts use of residual insecticide sprays to domestic dwellings. Our study suggests substantial benefits of extending the approach to treatment of cattle sheds, or deploying other tactics that target zoophilic behavior. Optimizing use of existing tools will be essential to achieving the ambitious 2030 elimination target. PMID:28091570

  5. Eliminate Sweetened Drinks, Cut Kids' Sugar Intake

    Science.gov (United States)

    ... fullstory_161163.html Eliminate Sweetened Drinks, Cut Kids' Sugar Intake Average U.S. child consumes about 80 grams ... Looking for the quickest way to cut added sugar from your kid's diet? Eliminate sugar-sweetened drinks, ...

  6. Quantifier Elimination by Dependency Sequents

    CERN Document Server

    Goldberg, Eugene

    2012-01-01

    We consider the problem of existential quantifier elimination for Boolean formulas in Conjunctive Normal Form (CNF). We present a new method for solving this problem called Derivation of Dependency-Sequents (DDS). A Dependency-sequent (D-sequent) is used to record that a set of quantified variables is redundant under a partial assignment. We show that D-sequents can be resolved to obtain new, non-trivial D-sequents. We also show that DDS is compositional, i.e. if our input formula is a conjunction of independent formulas, DDS automatically recognizes and exploits this information. We introduce an algorithm based on DDS and present experimental results demonstrating its potential.

  7. Elimination communication as colic therapy.

    Science.gov (United States)

    Jordan, Geraldine J

    2014-09-01

    Colic is generally defined as excessive crying in early infancy and can have negative consequences on the infant as well as on the infant's family life. Excessive crying can result in escalating parental stress levels, abusive caregiver response, increased risk of shaken baby syndrome and parental postpartum depression. In addition to excessive crying, symptoms and descriptors of infant colic include inconsolable crying, screaming, legs drawn up against the abdomen, furrowing of eyebrows, distended abdomen, arched back, passing gas, post-feeding crying and difficulty defecating. There are few well-designed, reproducible, randomized, large-scale studies which demonstrate efficacy of any therapeutic method for colic. An unexplored etiology is that colic is functionally related to a decrease in stooling frequency. Gut distention may periodically result in intensifying discomfort for the infant and in concomitant inconsolable crying. Elimination communication (EC; also known as Natural Infant Hygiene and sometimes referred to as infant potty training, baby-led potty training or assisted infant toilet training) involves the use of cues by which the infant signals to the caregiver that the infant needs to micturate or defecate. Such cues can include types of crying, squirming, straining, wriggling, grimacing, fussing, vocalizing, intent look at caregiver, red face, passing gas and grunting, many of which are the same initial symptoms related to the onset of colicky infant states. A caregiver's attentive and nurturant response to an infant's cues involve uncovering the infant's intergluteal cleft and cradling the infant gently and non-coercively in a supported, secure squatting position. This position will increase the infant's anorectal angle thus facilitating complete defecation. It is hypothesized that effective and timely elimination will cause increased physical comfort for the infant; colic symptoms will concomitantly decrease.

  8. Ability of clinicopathologic variables and clinical examination findings to predict race elimination in endurance horses.

    Science.gov (United States)

    Fielding, C Langdon; Meier, Chloe A; Fellers, Greg K; Magdesian, K Gary

    2017-01-01

    OBJECTIVE To compare results of point-of-care laboratory testing with standard veterinary clinical examination findings at a single time point during endurance competition to identify horses at risk for elimination. ANIMALS 101 endurance horses participating in the 2013 Western States 160-km (100-mile) endurance ride. PROCEDURES At the 58-km checkpoint, blood samples were collected from all horses. Samples were analyzed for pH, Pco2, base excess, anion gap, PCV, and whole blood concentrations of sodium, potassium, chloride, total carbon dioxide, BUN, glucose, and bicarbonate. Corrected electrolyte and PCV values were calculated on the basis of plasma total protein concentration. Immediately following the blood sample collection, each horse underwent a clinical examination. In addition to standard examination variables, an adjusted heart rate was calculated on the basis of the variable interval between entry into the checkpoint and heart rate recording. A combination of stepwise logistic regression, classification and regression tree analysis, and generalized additive models was used to identify variables that were associated with overall elimination or each of 3 other elimination categories (metabolic elimination, lameness elimination, and elimination for other reasons). RESULTS Corrected whole blood potassium concentration and adjusted heart rate were predictive for overall elimination. Breed, plasma total protein concentration, and attitude were predictive for elimination due to metabolic causes. Whole blood chloride concentration and corrected PCV were predictive for elimination due to lameness. Corrected PCV was predictive for elimination due to other causes. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that for horses in endurance competition, a combination of breed and clinical examination and laboratory variables provided the best prediction of overall elimination.

  9. Cosmic Ray elimination using the Wavelet Transform

    Science.gov (United States)

    Orozco-Aguilera, M. T.; Cruz, J.; Altamirano, L.; Serrano, A.

    2009-11-01

    In this work, we present a method for the automatic cosmic ray elimination in a single CCD exposure using the Wavelet Transform. The proposed method can eliminate cosmic rays of any shape or size. With this method we can eliminate over 95% of cosmic rays in a spectral image.

  10. COSMIC RAY ELIMINATION USING THE WAVELET TRANSFORM

    Directory of Open Access Journals (Sweden)

    M. T. Orozco-Aguilera

    2009-01-01

    Full Text Available In this work, we present a method for the automatic cosmic ray elimination in a single CCD exposure using the Wavelet Transform. The proposed method can eliminate cosmic rays of any shape or size. With this method we can eliminate over 95% of cosmic rays in a spectral image.

  11. Atmospheric Entry Experiments at IRS

    Science.gov (United States)

    Auweter-Kurtz, M.; Endlich, P.; Herdrich, G.; Kurtz, H.; Laux, T.; Löhle, S.; Nazina, N.; Pidan, S.

    2002-01-01

    Entering the atmosphere of celestial bodies, spacecrafts encounter gases at velocities of several km/s, thereby being subjected to great heat loads. The thermal protection systems and the environment (plasma) have to be investigated by means of computational and ground facility based simulations. For more than a decade, plasma wind tunnels at IRS have been used for the investigation of TPS materials. Nevertheless, ground tests and computer simulations cannot re- place space flights completely. Particularly, entry mission phases encounter challenging problems, such as hypersonic aerothermodynamics. Concerning the TPS, radiation-cooled materials used for reuseable spacecrafts and ablator tech- nologies are of importance. Besides the mentioned technologies, there is the goal to manage guidance navigation, con- trol, landing technology and inflatable technologies such as ballutes that aim to keep vehicles in the atmosphere without landing. The requirement to save mass and energy for planned interplanetary missions such as Mars Society Balloon Mission, Mars Sample Return Mission, Mars Express or Venus Sample Return mission led to the need for manoeuvres like aerocapture, aero-breaking and hyperbolic entries. All three are characterized by very high kinetic vehicle energies to be dissipated by the manoeuvre. In this field flight data are rare. The importance of these manoeuvres and the need to increase the knowledge of required TPS designs and behavior during such mission phases point out the need of flight experiments. As result of the experience within the plasma diagnostic tool development and the plasma wind tunnel data base, flight experiments like the PYrometric RE-entry EXperiment PYREX were developed, fully qualified and successfully flown. Flight experiments such as the entry spectrometer RESPECT and PYREX on HOPE-X are in the conceptual phase. To increase knowledge in the scope of atmospheric manoeuvres and entries, data bases have to be created combining both

  12. Menstrual Cycle

    Science.gov (United States)

    ... Luteal (after egg release) Changes During the Menstrual Cycle The menstrual cycle is regulated by the complex interaction of ... egg release) Luteal (after egg release) The menstrual cycle begins with menstrual bleeding (menstruation), which marks the first day of ...

  13. Drag-based composite super-twisting sliding mode control law design for Mars entry guidance

    Science.gov (United States)

    Zhao, Zhenhua; Yang, Jun; Li, Shihua; Guo, Lei

    2016-06-01

    In this paper, the drag-based trajectory tracking guidance problem is investigated for Mars entry vehicle subject to uncertainties. A composite super twisting sliding mode control method based on finite-time disturbance observer is proposed for guidance law design. The proposed controller not only eliminates the effects of matched and mismatched disturbances due to uncertainties of atmospheric models and vehicle aerodynamics but also guarantees the continuity of control action. Numerical simulations are carried out on the basis of Mars Science Laboratory mission, where the results show that the proposed methods can improve the Mars entry guidance precision as compared with some existing guidance methods including PID and ADRC.

  14. Matrix Completion from Noisy Entries

    CERN Document Server

    Keshavan, Raghunandan H; Oh, Sewoong

    2009-01-01

    Given a matrix M of low-rank, we consider the problem of reconstructing it from noisy observations of a small, random subset of its entries. The problem arises in a variety of applications, from collaborative filtering (the `Netflix problem') to structure-from-motion and positioning. We study a low complexity algorithm introduced by Keshavan et al.(2009), based on a combination of spectral techniques and manifold optimization, that we call here OptSpace. We prove performance guarantees that are order-optimal in a number of circumstances.

  15. Sodium hydrogen exchangers contribute to arenavirus cell entry.

    Science.gov (United States)

    Iwasaki, Masaharu; Ngo, Nhi; de la Torre, Juan C

    2014-01-01

    Several arenaviruses, chiefly Lassa virus (LASV), cause hemorrhagic fever (HF) disease in humans and pose a great public health concern in the regions in which they are endemic. Moreover, evidence indicates that the worldwide-distributed prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a neglected human pathogen of clinical significance. The limited existing armamentarium to combat human-pathogenic arenaviruses underscores the importance of developing novel antiarenaviral drugs, a task that would be facilitated by the identification and characterization of virus-host cell factor interactions that contribute to the arenavirus life cycle. A genome-wide small interfering RNA (siRNA) screen identified sodium hydrogen exchanger 3 (NHE3) as required for efficient multiplication of LCMV in HeLa cells, but the mechanisms by which NHE activity contributed to the life cycle of LCMV remain unknown. Here we show that treatment with the NHE inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA) resulted in a robust inhibition of LCMV multiplication in both rodent (BHK-21) and human (A549) cells. EIPA-mediated inhibition was due not to interference with virus RNA replication, gene expression, or budding but rather to a blockade of virus cell entry. EIPA also inhibited cell entry mediated by the glycoproteins of the HF arenaviruses LASV and Junin virus (JUNV). Pharmacological and genetic studies revealed that cell entry of LCMV in A549 cells depended on actin remodeling and Pak1, suggesting a macropinocytosis-like cell entry pathway. Finally, zoniporide, an NHE inhibitor being explored as a therapeutic agent to treat myocardial infarction, inhibited LCMV propagation in culture cells. Our findings indicate that targeting NHEs could be a novel strategy to combat human-pathogenic arenaviruses.

  16. Adaptive Text Entry for Mobile Devices

    DEFF Research Database (Denmark)

    Proschowsky, Morten Smidt

    for mobile devices and a framework for adaptive context-aware language models. Based on analysis of current text entry methods, the requirements to the new text entry methods are established. Transparent User guided Prediction (TUP) is a text entry method for devices with one dimensional touch input. It can......The reduced size of many mobile devices makes it difficult to enter text with them. The text entry methods are often slow or complicated to use. This affects the performance and user experience of all applications and services on the device. This work introduces new easy-to-use text entry methods...... to improve the models of human motor behaviour. TUP-Key is a variant of TUP, designed for 12 key phone keyboards. It is introduced in the thesis but has not been implemented or evaluated. Both text entry methods support adaptive context-aware language models. YourText is a framework for adaptive context...

  17. Preventing re-entry to foster care.

    Science.gov (United States)

    Carnochan, Sarah; Rizik-Baer, Daniel; Austin, Michael J

    2013-01-01

    Re-entry to foster care generally refers to circumstances in which children who have been discharged from foster care to be reunified with their family of origin, adopted, or provided kinship guardianship are returned to foster care. In the context of the federal performance measurement system, re-entry refers specifically to a return to foster care following an unsuccessful reunification. The federal Children and Family Services Review measures re-entry to foster care with a single indicator, called the permanency of reunification indicator, one of four indicators comprising the reunification composite measure. This review focuses on research related to the re-entry indicator, including the characteristics of children, caregivers and families, as well as case and child welfare services that are associated with a higher or lower risk of re-entry to foster care. Promising post-reunification services designed to prevent re-entry to foster care are described.

  18. Bailey mine eliminates cribbing in the tailgates

    Energy Technology Data Exchange (ETDEWEB)

    McCaffrey, J.J.; Stankus, J.C.; Peng, S.S. (Enlow Fork Mine (United States))

    1994-05-01

    Describes a new method of tailgate support, designed as an alternative to modern crib support, and tested in an active longwall panel at the Bailey mine, Greene County, Pennsylvania. The new system works in conjunction with the existing primary support roof bolts and employs JM trusses from Jennmar Corp. installed at 4 ft intervals in the tailgate entry and 2 ft intervals in each crosscut. The entry was monitored prior to and during the successful longwall retreat through the test area using vibrating wire stress meters, convergence stations, strain gages, calibrated load cells and stratascope boreholes. There were no roof control problems in the tailgate entry and the trusses successfully controlled the abutment pressures, allowing the tailgate entry to remain open throughout the mining period. 3 figs., 4 photos.

  19. Elimination of Onchocerciasis from Mexico.

    Directory of Open Access Journals (Sweden)

    Mario A Rodríguez-Pérez

    Full Text Available Mexico is one of the six countries formerly endemic for onchocerciasis in Latin America. Transmission has been interrupted in the three endemic foci of that country and mass drug distribution has ceased. Three years after mass drug distribution ended, post-treatment surveillance (PTS surveys were undertaken which employed entomological indicators to check for transmission recrudescence.In-depth entomologic assessments were performed in 18 communities in the three endemic foci of Mexico. None of the 108,212 Simulium ochraceum s.l. collected from the three foci were found to contain parasite DNA when tested by polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA, resulting in a maximum upper bound of the 95% confidence interval (95%-ULCI of the infective rate in the vectors of 0.035/2,000 flies examined. This is an order of magnitude below the threshold of a 95%-ULCI of less than one infective fly per 2,000 flies tested, the current entomological criterion for interruption of transmission developed by the international community. The point estimate of seasonal transmission potential (STP was zero, and the upper bound of the 95% confidence interval for the STP ranged from 1.2 to 1.7 L3/person/season in the different foci. This value is below all previous estimates for the minimum transmission potential required to maintain the parasite population.The results from the in-depth entomological post treatment surveillance surveys strongly suggest that transmission has not resumed in the three foci of Mexico during the three years since the last distribution of ivermectin occurred; it was concluded that transmission remains undetectable without intervention, and Onchocerca volvulus has been eliminated from Mexico.

  20. Replication of Alphaviruses: A Review on the Entry Process of Alphaviruses into Cells

    Directory of Open Access Journals (Sweden)

    Jason Yat-Sing Leung

    2011-01-01

    Full Text Available Alphaviruses are small, enveloped viruses, ~70 nm in diameter, containing a single-stranded, positive-sense, RNA genome. Viruses belonging to this genus are predominantly arthropod-borne viruses, known to cause disease in humans. Their potential threat to human health was most recently exemplified by the 2005 Chikungunya virus outbreak in La Reunion, highlighting the necessity to understand events in the life-cycle of these medically important human pathogens. The replication and propagation of viruses is dependent on entry into permissive cells. Viral entry is initiated by attachment of virions to cells, leading to internalization, and uncoating to release genetic material for replication and propagation. Studies on alphaviruses have revealed entry via a receptor-mediated, endocytic pathway. In this paper, the different stages of alphavirus entry are examined, with examples from Semliki Forest virus, Sindbis virus, Chikungunya virus, and Venezuelan equine encephalitis virus described.

  1. The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.

    Science.gov (United States)

    Moghe, Saili; Jiang, Fei; Miura, Yoshie; Cerny, Ronald L; Tsai, Ming-Ying; Furukawa, Manabu

    2012-02-15

    The cullin-RING family of ubiquitin ligases regulates diverse cellular functions, such as cell cycle control, via ubiquitylation of specific substrates. CUL3 targets its substrates through BTB proteins. Here we show that depletion of CUL3 and the BTB protein KLHL18 causes a delay in mitotic entry. Centrosomal activation of Aurora-A, a kinase whose activity is required for entry into mitosis, is also delayed in depleted cells. Moreover, we identify Aurora-A as a KLHL18-interacting partner. Overexpression of KLHL18 and CUL3 promotes Aurora-A ubiquitylation in vivo, and the CUL3-KLHL18-ROC1 ligase ubiquitylates Aurora-A in vitro. Our study reveals that the CUL3-KLHL18 ligase is required for timely entry into mitosis, as well as for the activation of Aurora-A at centrosomes. We propose that the CUL3-KLHL18 ligase regulates mitotic entry through an Aurora-A-dependent pathway.

  2. The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A

    Directory of Open Access Journals (Sweden)

    Saili Moghe

    2012-02-01

    The cullin-RING family of ubiquitin ligases regulates diverse cellular functions, such as cell cycle control, via ubiquitylation of specific substrates. CUL3 targets its substrates through BTB proteins. Here we show that depletion of CUL3 and the BTB protein KLHL18 causes a delay in mitotic entry. Centrosomal activation of Aurora-A, a kinase whose activity is required for entry into mitosis, is also delayed in depleted cells. Moreover, we identify Aurora-A as a KLHL18-interacting partner. Overexpression of KLHL18 and CUL3 promotes Aurora-A ubiquitylation in vivo, and the CUL3-KLHL18-ROC1 ligase ubiquitylates Aurora-A in vitro. Our study reveals that the CUL3-KLHL18 ligase is required for timely entry into mitosis, as well as for the activation of Aurora-A at centrosomes. We propose that the CUL3-KLHL18 ligase regulates mitotic entry through an Aurora-A-dependent pathway.

  3. Mating ecology explains patterns of genome elimination.

    Science.gov (United States)

    Gardner, Andy; Ross, Laura

    2014-12-01

    Genome elimination - whereby an individual discards chromosomes inherited from one parent, and transmits only those inherited from the other parent - is found across thousands of animal species. It is more common in association with inbreeding, under male heterogamety, in males, and in the form of paternal genome elimination. However, the reasons for this broad pattern remain unclear. We develop a mathematical model to determine how degree of inbreeding, sex determination, genomic location, pattern of gene expression and parental origin of the eliminated genome interact to determine the fate of genome-elimination alleles. We find that: inbreeding promotes paternal genome elimination in the heterogametic sex; this may incur population extinction under female heterogamety, owing to eradication of males; and extinction is averted under male heterogamety, owing to countervailing sex-ratio selection. Thus, we explain the observed pattern of genome elimination. Our results highlight the interaction between mating system, sex-ratio selection and intragenomic conflict.

  4. Hepatitis C virus entry and the tetraspanin CD81.

    Science.gov (United States)

    Farquhar, Michelle J; Harris, Helen J; McKeating, Jane A

    2011-04-01

    CD81, a member of the tetraspanin integral membrane protein family, has been identified as an essential receptor for HCV (hepatitis C virus). The present review highlights recent published data on the role that CD81 plays in HCV entry, including the importance of actin-dependent lateral diffusion of CD81 within the cell membrane, CD81 endocytosis and the CD81-Claudin-1 receptor complex in HCV internalization. Additional functions for CD81 in the viral life cycle and the role of HCV-CD81 interactions in HCV-induced B-cell and CNS (central nervous system) abnormalities are discussed.

  5. Border Crossing/Entry Data - Border Crossing/Entry Data Time Series tool

    Data.gov (United States)

    Department of Transportation — The dataset is known as “Border Crossing/Entry Data.” The Bureau of Transportation Statistics (BTS) Border Crossing/Entry Data provides summary statistics to the...

  6. Entry Into a Care Trajectory

    Directory of Open Access Journals (Sweden)

    Normand Carpentier

    2013-06-01

    Full Text Available A broad range of services are developing in response to the needs of an aging population. Although most interventions are carried out in the patient’s living environment—at the heart of society—few studies on service utilization refer to social theory. This paper suggests that studies on older people with chronic health conditions would benefit from a stronger theoretical foundation. Drawing on 15 in-depth interviews, it highlights the relevance of individualization processes and the network society, 2 central theoretical concerns in sociology. The research provides a unique perspective on the entry into the care trajectory and expands our comprehension of the emergence of a social organization that can respond to the needs of elderly patients. Data of this nature may be useful in service planning and the development of a care-centered approach.

  7. Elimination of onchocerciasis from Africa: possible?

    Science.gov (United States)

    Mackenzie, Charles D; Homeida, Mamoun M; Hopkins, Adrian D; Lawrence, Joni C

    2012-01-01

    Human onchocerciasis, a parasitic disease found in 28 African countries, six Latin American countries and Yemen, causes blindness and severe dermatological problems. In 1987, efforts to control this infection shifted from vector approaches to include the mass distribution of ivermectin - a drug donated by Merck & Co. for disease control in Africa and for disease elimination in the Americas. Currently, almost 25 years later, with the Americas being highly successful and now approaching elimination, new evidence points towards the possibility of successful elimination in Africa. We suggest several major changes in the programmatic approach that through focused goal-directed effort could achieve global elimination of onchocerciasis by 2025.

  8. Recent Advances in Hepatitis C Virus Cell Entry

    Directory of Open Access Journals (Sweden)

    Jean Dubuisson

    2010-03-01

    Full Text Available More than 170 million patients worldwide are chronically infected with hepatitis C virus (HCV. Prevalence rates range from 0.5% in Northern European countries to 28% in some areas of Egypt. HCV is hepatotropic, and in many countries chronic hepatitis C is a leading cause of liver disease including fibrosis, cirrhosis and hepatocellular carcinoma. HCV persists in 50–85% of infected patients, and once chronic infection is established, spontaneous clearance is rare. HCV is a member of the Flaviviridae family, in which it forms its own genus. Many lines of evidence suggest that the HCV life cycle displays many differences to that of other Flaviviridae family members. Some of these differences may be due to the close interaction of HCV with its host’s lipid and particular triglyceride metabolism in the liver, which may explain why the virus can be found in association with lipoproteins in serum of infected patients. This review focuses on the molecular events underlying the HCV cell entry process and the respective roles of cellular co-factors that have been implied in these events. These include, among others, the lipoprotein receptors low density lipoprotein receptor and scavenger receptor BI, the tight junction factors occludin and claudin-1 as well as the tetraspanin CD81. We discuss the roles of these cellular factors in HCV cell entry and how association of HCV with lipoproteins may modulate the cell entry process.

  9. Hitting a Moving Target: A Model for Malaria Elimination in the Presence of Population Movement.

    Directory of Open Access Journals (Sweden)

    Sheetal Prakash Silal

    Full Text Available South Africa is committed to eliminating malaria with a goal of zero local transmission by 2018. Malaria elimination strategies may be unsuccessful if they focus only on vector biology, and ignore the mobility patterns of humans, particularly where the majority of infections are imported. In the first study in Mpumalanga Province in South Africa designed for this purpose, a metapopulation model is developed to assess the impact of their proposed elimination-focused policy interventions. A stochastic, non-linear, ordinary-differential equation model is fitted to malaria data from Mpumalanga and neighbouring Maputo Province in Mozambique. Further scaling-up of vector control is predicted to lead to a minimal reduction in local infections, while mass drug administration and focal screening and treatment at the Mpumalanga-Maputo border are predicted to have only a short-lived impact. Source reduction in Maputo Province is predicted to generate large reductions in local infections through stemming imported infections. The mathematical model predicts malaria elimination to be possible only when imported infections are treated before entry or eliminated at the source suggesting that a regionally focused strategy appears needed, for achieving malaria elimination in Mpumalanga and South Africa.

  10. Foreign Entry and Heterogeneous Growth of Firms

    DEFF Research Database (Denmark)

    Deng, Paul Duo; Jefferson, Gary H.

    We adopt the framework of Schumpeterian creative destruction formalized by Aghion et al. (2009) to analyze the impact of foreign entry on the productivity growth of domestic firms. In the face of foreign entry, domestic firms exhibit heterogeneous patterns of growth depending on their technological...

  11. Predicting the Diversity of Foreign Entry Modes

    DEFF Research Database (Denmark)

    Hashai, Niron; Geisler Asmussen, Christian; Benito, Gabriel;

    2007-01-01

    diversity across value chain activities and host markets. Analyzing a sample of Israeli based firms we show that larger firms exhibit a higher degree of entry mode diversity both across value chain activities and across host markets. Higher levels of knowledge intensity are also associated with more...... diversity in firms' entry modes across both dimensions....

  12. Foreign Entry and Heterogeneous Growth of Firms

    DEFF Research Database (Denmark)

    Deng, Paul Duo; Jefferson, Gary H.

    We adopt the framework of Schumpeterian creative destruction formalized by Aghion et al. (2009) to analyze the impact of foreign entry on the productivity growth of domestic firms. In the face of foreign entry, domestic firms exhibit heterogeneous patterns of growth depending on their technologic...

  13. Dimerization and nuclear entry of mPER proteins in mammalian cells

    NARCIS (Netherlands)

    K. Yagita (Kazuhiro); S. Yamaguchi; F. Tamanini (Filippo); A. Yasui (Akira); J.J. Loros; J.C. Dunlap; H. Okamura (Hitoshi); J.H.J. Hoeijmakers (Jan); G.T.J. van der Horst (Gijsbertus)

    2000-01-01

    textabstractNuclear entry of circadian oscillatory gene products is a key step for the generation of a 24-hr cycle of the biological clock. We have examined nuclear import of clock proteins of the mammalian period gene family and the effect of serum shock, which induces a synchrono

  14. Endogenous, Imperfectly Competitive Business Cycles

    DEFF Research Database (Denmark)

    Whitta-Jacobsen, Hans Jørgen

    by monopolistic competition. An implicit assumption of barriers to entry justifies that the number of firms is fixed even when positive profits occur. It turns out that both market power of firms on the product markets and market power of unions on the labor markets make the occurrence of cycles more likely......We investigate how imperfect competition affects the occurrence and the properties of endogenous, rational expectations business cycles in an overlapping generations model with constant returns to scale in production. The model has explicit product and labor markets all characterized....... In particular, imperfect competition on the product markets and the positive profits associated with it may have the effect that there is a cycle even if the labor supply curve is increasing in the real-wage rate. For competitive cycles is required not only a decreasing labor supply curve, but a wage elasticity...

  15. Biogeochemical Cycling

    Science.gov (United States)

    Bebout, Brad; Fonda, Mark (Technical Monitor)

    2002-01-01

    This lecture will introduce the concept of biogeochemical cycling. The roles of microbes in the cycling of nutrients, production and consumption of trace gases, and mineralization will be briefly introduced.

  16. Thermal Soak Analysis of Earth Entry Vehicles

    Science.gov (United States)

    Agrawal, Parul; Sepka, Steven A.; Aliaga, Jose F.; Venkatapathy, Ethiraj; Samareh, Jamshid A.

    2012-01-01

    The Multi-Mission Earth Entry Vehicle project is developing an integrated tool called Multi Mission System Analysis for Planetary Entry Descent and Landing that will provide key technology solutions including mass sizing, aerodynamics, aerothermodynamics, and thermal and structural analysis for any given sample return mission. Thermal soak analysis and temperature predictions of various components including the payload container of the entry vehicle are part of the solution that this tool will offer to mission designers. The present paper focuses on the thermal soak analysis of an entry vehicle design based on the Mars Sample Return entry vehicle geometry and discusses a technical approach to develop parametric models for thermal soak analysis that will be integrated into the tool.

  17. Towards malaria elimination - a new thematic series

    Directory of Open Access Journals (Sweden)

    Tanner Marcel

    2010-01-01

    Full Text Available Abstract The launch of a new thematic series of Malaria Journal -- "Towards malaria elimination" -- creates the forum that allows carrying scientific evidence on how to achieve malaria elimination in specific endemic settings and conditions into the circles of scientists, public health specialists, national and global programme managers, funders and decision makers.

  18. A Brief Discussion of eliminating Grammatical Ambiguity

    Institute of Scientific and Technical Information of China (English)

    张俊

    2014-01-01

    As we all know that one kind of language structure has more than one meaning,to be called ambiguity.Generally thought,the grammatical ambiguity is quite complex in English,and this article pays attention to eliminating English grammatical ambiguity and making the shallow analysis on the three kinds of strategies of eliminating ambiguity.

  19. A Brief Discussion of eliminating Grammatical Ambiguity

    Institute of Scientific and Technical Information of China (English)

    张俊

    2014-01-01

    As we all know that one kind of language structure has more than one meaning, to be called ambiguity.Generally thought, the grammatical ambiguity is quite complex in English ,and this article pays attention to eliminating English grammatical ambiguity and making the shallow analysis on the three kinds of strategies of eliminating ambiguity.

  20. Parallelisation of surface-related multiple elimination

    NARCIS (Netherlands)

    vanWaveren, GM; Godfrey, IM; Hertzberger, B; Serazzi, G

    1995-01-01

    This paper presents the first published parallelisation of the surface-related multiple elimination method from the Delphi (3) software release. This method is used in the seismic industry to eliminate multiple data from recorded seismic data. Both data-parallel and message-passing implementation sc

  1. Improvement of the stone elimination roller mill

    Institute of Scientific and Technical Information of China (English)

    SunGuofeng

    2005-01-01

    Elimination roller mill as raw materials preparing equipments is universally used in brick making industry. Stone Elimination Roller Mill should have following characteristics: high machine strength and good wearresistant of roller shell, safety reliability, high machinery intensity (impact strength) and rigidity, reliable hermetically sealed construction.

  2. 19 CFR 142.3 - Entry documentation required.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry documentation required. 142.3 Section 142.3... TREASURY (CONTINUED) ENTRY PROCESS Entry Documentation § 142.3 Entry documentation required. (a) Contents. Except as provided in paragraph (b) of this section, the entry documentation required to secure...

  3. Clause Elimination Procedures for CNF Formulas

    Science.gov (United States)

    Heule, Marijn; Järvisalo, Matti; Biere, Armin

    We develop and analyze clause elimination procedures, a specific family of simplification techniques for conjunctive normal form (CNF) formulas. Extending known procedures such as tautology, subsumption, and blocked clause elimination, we introduce novel elimination procedures based on hidden and asymmetric variants of these techniques. We analyze the resulting nine (including five new) clause elimination procedures from various perspectives: size reduction, BCP-preservance, confluence, and logical equivalence. For the variants not preserving logical equivalence, we show how to reconstruct solutions to original CNFs from satisfying assignments to simplified CNFs. We also identify a clause elimination procedure that does a transitive reduction of the binary implication graph underlying any CNF formula purely on the CNF level.

  4. LABORATORY VOICE DATA ENTRY SYSTEM.

    Energy Technology Data Exchange (ETDEWEB)

    PRAISSMAN,J.L.SUTHERLAND,J.C.

    2003-04-01

    We have assembled a system using a personal computer workstation equipped with standard office software, an audio system, speech recognition software and an inexpensive radio-based wireless microphone that permits laboratory workers to enter or modify data while performing other work. Speech recognition permits users to enter data while their hands are holding equipment or they are otherwise unable to operate a keyboard. The wireless microphone allows unencumbered movement around the laboratory without a ''tether'' that might interfere with equipment or experimental procedures. To evaluate the potential of voice data entry in a laboratory environment, we developed a prototype relational database that records the disposal of radionuclides and/or hazardous chemicals Current regulations in our laboratory require that each such item being discarded must be inventoried and documents must be prepared that summarize the contents of each container used for disposal. Using voice commands, the user enters items into the database as each is discarded. Subsequently, the program prepares the required documentation.

  5. Novel Approaches to Inhibit HIV Entry

    Directory of Open Access Journals (Sweden)

    Chukwuka A. Didigu

    2012-02-01

    Full Text Available Human Immunodeficiency Virus (HIV entry into target cells is a multi-step process involving binding of the viral glycoprotein, Env, to its receptor CD4 and a coreceptor—either CCR5 or CXCR4. Understanding the means by which HIV enters cells has led to the identification of genetic polymorphisms, such as the 32 base-pair deletion in the ccr5 gene (ccr5∆32 that confers resistance to infection in homozygous individuals, and has also resulted in the development of entry inhibitors—small molecule antagonists that block infection at the entry step. The recent demonstration of long-term control of HIV infection in a leukemic patient following a hematopoietic stem cell transplant using cells from a ccr5∆32 homozygous donor highlights the important role of the HIV entry in maintaining an established infection and has led to a number of attempts to treat HIV infection by genetically modifying the ccr5 gene. In this review, we describe the HIV entry process and provide an overview of the different classes of approved HIV entry inhibitors while highlighting novel genetic strategies aimed at blocking HIV infection at the level of entry.

  6. A new global strategy for the elimination of schistosomiasis.

    Science.gov (United States)

    Ross, Allen G P; Chau, Thao N; Inobaya, Marianette T; Olveda, Remigio M; Li, Yuesheng; Harn, Donald A

    2017-01-01

    Mass drug administration utilising a single oral dose of 40mg/kg of praziquantel (PZQ) has been endorsed and advocated by the World Health Organisation (WHO) for the global control and elimination of schistosomiasis. However, this strategy is failing primarily because the drugs are not getting to the people who need them the most. The current global coverage is 20%, the drug compliance rate is less than 50%, and the drug efficacy is approximately 50%. Thus in reality, only about 5% of the reservoir human population is actually receiving intermittent chemotherapy. Despite claims that more of the drug will soon be made available the current strategy is inherently flawed and will not lead to disease elimination. We discuss the many practical issues related to this global strategy, and advocate for an integrated control strategy targeting the life cycle and the most at-risk. Moreover, we discuss how an integrated control package for schistosomiasis should fit within a larger integrated health package for rural and remote villages in the developing world. A holistic health system approach is required to achieve sustainable control and ultimately disease elimination.

  7. Modeling selective elimination of quiescent cancer cells from bone marrow.

    Science.gov (United States)

    Cavnar, Stephen P; Rickelmann, Andrew D; Meguiar, Kaille F; Xiao, Annie; Dosch, Joseph; Leung, Brendan M; Cai Lesher-Perez, Sasha; Chitta, Shashank; Luker, Kathryn E; Takayama, Shuichi; Luker, Gary D

    2015-08-01

    Patients with many types of malignancy commonly harbor quiescent disseminated tumor cells in bone marrow. These cells frequently resist chemotherapy and may persist for years before proliferating as recurrent metastases. To test for compounds that eliminate quiescent cancer cells, we established a new 384-well 3D spheroid model in which small numbers of cancer cells reversibly arrest in G1/G0 phase of the cell cycle when cultured with bone marrow stromal cells. Using dual-color bioluminescence imaging to selectively quantify viability of cancer and stromal cells in the same spheroid, we identified single compounds and combination treatments that preferentially eliminated quiescent breast cancer cells but not stromal cells. A treatment combination effective against malignant cells in spheroids also eliminated breast cancer cells from bone marrow in a mouse xenograft model. This research establishes a novel screening platform for therapies that selectively target quiescent tumor cells, facilitating identification of new drugs to prevent recurrent cancer. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

  8. Cut elimination in multifocused linear logic

    DEFF Research Database (Denmark)

    Guenot, Nicolas; Brock-Nannestad, Taus

    2015-01-01

    We study cut elimination for a multifocused variant of full linear logic in the sequent calculus. The multifocused normal form of proofs yields problems that do not appear in a standard focused system, related to the constraints in grouping rule instances in focusing phases. We show that cut...... elimination can be performed in a sensible way even though the proof requires some specific lemmas to deal with multifocusing phases, and discuss the difficulties arising with cut elimination when considering normal forms of proofs in linear logic....

  9. Cut elimination in multifocused linear logic

    DEFF Research Database (Denmark)

    Guenot, Nicolas; Brock-Nannestad, Taus

    2015-01-01

    We study cut elimination for a multifocused variant of full linear logic in the sequent calculus. The multifocused normal form of proofs yields problems that do not appear in a standard focused system, related to the constraints in grouping rule instances in focusing phases. We show that cut elim...... elimination can be performed in a sensible way even though the proof requires some specific lemmas to deal with multifocusing phases, and discuss the difficulties arising with cut elimination when considering normal forms of proofs in linear logic....

  10. Advances in spacecraft atmospheric entry guidance

    Science.gov (United States)

    Benito Manrique, Joel

    In order to advance entry guidance technology two different research areas have been explored with the objective of increasing the reachable landing area and the landing accuracy for future Mars missions. Currently only the northern hemisphere of Mars is available for landing due to its low elevation. Only low elevation landing sites have the necessary atmospheric density to allow landing using current Entry, Descent and Landing (EDL) technology. In order to reach most of the Ancient Highlands, the majority of the southern hemisphere, advanced EDL technology is needed in multiple fields, including entry guidance. The first research area is the definition and applications of reachable and controllable sets for entry. The definition of the reachable and controllable sets provides a framework for the study of the capabilities of an entry vehicle in a given planet. Reachable and controllable sets can be used to comprehensively characterize the envelope of trajectories that a vehicle can fly, the sites it can reach and the entry states that can be accommodated. The sets can also be used for the evaluation of trajectory planning algorithms and to assist in the selection of the entry or landing sites. In essence, the reachable and controllable sets offer a powerful vehicle and trajectory analysis and design framework that allows for better mission design choices. In order to illustrate the use of the sets, they are computed for a representative Mars mission using two different vehicle configurations. The sets characterize the impact of the vehicle configuration on the entry capability. Furthermore, the sets are used to find the best skip-entry trajectory for a return from the Moon mission, highlighting the utility of the sets in atmospheric maneuvers other than entry. The second research area is the development of the components of an entry guidance algorithm that allow high elevation landing and provide as well high landing accuracy. The approach taken follows the

  11. Entry Regulation under Asymmetric Information about Demand

    Directory of Open Access Journals (Sweden)

    Paula Sarmento

    2010-01-01

    Full Text Available We investigate how an incumbent firm can use the regulatory policy about entry and the informational advantage to protect his market position. This question is studied through the construction of a signalling game where we assume that the regulator has less information about demand than the firms. We conclude that there is a pooling equilibrium and partially separating equilibria in which entry is deterred and, if demand is high, there will be insufficient entry. The final effect on welfare depends on the tradeoff between short-run benefits (lower price and long-run losses (weaker competition.

  12. Market entry strategies into the BRIC countries

    DEFF Research Database (Denmark)

    Boyd, Britta; Dyhr Ulrich, Anna Marie

    2014-01-01

    Based on a sample of 177 exporting SMEs, this study investigates what market entry strategy is used by Danish family and non-family businesses. From a resource-based view, three critical internal factors (risk, flexibility and control) affecting the entry mode choice into the BRIC markets...... when entering the BRIC markets. In contrast, family firms choose high commitment entry modes which involve high risk and low flexibility when entering the BRIC markets. Further implications discuss the suitability of export strategies to BRIC markets for managers of Danish family and non-family firms....

  13. Descriptive epidemiology and risk factors for eliminations from Fédération Equestre Internationale endurance rides due to lameness and metabolic reasons (2008-2011).

    Science.gov (United States)

    Nagy, A; Murray, J K; Dyson, S J

    2014-01-01

    There is limited information on international endurance rides and on risk factors for eliminations. To describe and compare the frequency of occurrence of elimination due to lameness and metabolic reasons from Fédération Equestre Internationale (FEI) endurance rides (≥100 km) during 2008-2011 in all countries and to assess risk factors for elimination due to lameness and metabolic reasons. Retrospective unmatched case-control study. Data collected from the FEI website included the following: the country where the ride was held; the year; the result for each horse-rider combination; the number of entries in each class; whether the class was restricted to young riders; and the winning speed. Descriptive data were summarised, and univariable and multivariable logistic regression analyses were performed to assess risk factors for eliminations due to lameness and metabolic reasons. Between 2008 and 2011, there were 30,741 horse starts at FEI endurance rides of 100-160 km distance in 47 countries. Of all started horses, 30% were eliminated for lameness. Eliminations for metabolic reasons were recorded for 8.7% of all started horses. There was a large range of winning speeds amongst countries (10.2-29.5 km/h). Multivariable analysis revealed that the risk of elimination due to lameness was significantly associated with the country where the ride was held, the year, the distance of the ride and the number of entries. Elimination for metabolic reasons was significantly associated with the country where the ride was held, the year and the number of entries. Differences existed among elimination rates at FEI endurance rides in different countries (2008-2011). Lameness was the most common reason for elimination globally. These results provide objective global data on endurance riding that can replace anecdotal information, which has been the base of opinions, decisions and regulations. The Summary is available in Chinese - see Supporting information. © 2013 EVJ Ltd.

  14. Texting while driving: is speech-based text entry less risky than handheld text entry?

    Science.gov (United States)

    He, J; Chaparro, A; Nguyen, B; Burge, R J; Crandall, J; Chaparro, B; Ni, R; Cao, S

    2014-11-01

    Research indicates that using a cell phone to talk or text while maneuvering a vehicle impairs driving performance. However, few published studies directly compare the distracting effects of texting using a hands-free (i.e., speech-based interface) versus handheld cell phone, which is an important issue for legislation, automotive interface design and driving safety training. This study compared the effect of speech-based versus handheld text entries on simulated driving performance by asking participants to perform a car following task while controlling the duration of a secondary text-entry task. Results showed that both speech-based and handheld text entries impaired driving performance relative to the drive-only condition by causing more variation in speed and lane position. Handheld text entry also increased the brake response time and increased variation in headway distance. Text entry using a speech-based cell phone was less detrimental to driving performance than handheld text entry. Nevertheless, the speech-based text entry task still significantly impaired driving compared to the drive-only condition. These results suggest that speech-based text entry disrupts driving, but reduces the level of performance interference compared to text entry with a handheld device. In addition, the difference in the distraction effect caused by speech-based and handheld text entry is not simply due to the difference in task duration.

  15. Gap Cycling for SWIFT

    CERN Document Server

    Corum, Curtis A; Snyder, Carl J; Garwood, Michael

    2013-01-01

    Purpose: SWIFT (SWeep Imaging with Fourier Transformation) is a non- Cartesian MRI method with unique features and capabilities. In SWIFT, radiofrequency (RF) excitation and reception are performed nearly simultaneously, by rapidly switching between transmit and receive during a frequency-swept RF pulse. Because both the transmitted pulse and data acquisition are simultaneously amplitude-modulated in SWIFT (in contrast to continuous RF excitation and uninterrupted data acquisition in more familiar MRI sequences), crosstalk between different frequency bands occurs in the data. This crosstalk leads to a "bulls-eye" artifact in SWIFT images. We present a method to cancel this inter-band crosstalk by cycling the pulse and receive gap positions relative to the un-gapped pulse shape. We call this strategy "gap cycling." Methods: We carry out theoretical analysis, simulation and experiments to characterize the signal chain, resulting artifacts, and their elimination for SWIFT. Results: Theoretical analysis reveals t...

  16. Stress Testing of the Philips 60W Replacement Lamp L Prize Entry

    Energy Technology Data Exchange (ETDEWEB)

    Poplawski, Michael E.; Ledbetter, Marc R.; Smith, Mark

    2012-04-24

    The Pacific Northwest National Laboratory, operated by Battelle for the U.S. Department of Energy, worked with Intertek to develop a procedure for stress testing medium screw-base light sources. This procedure, composed of alternating stress cycles and performance evaluation, was used to qualitatively compare and contrast the durability and reliability of the Philips 60W replacement lamp L Prize entry with market-proven compact fluorescent lamps (CFLs) with comparable light output and functionality. The stress cycles applied simultaneous combinations of electrical, thermal, vibration, and humidity stresses of increasing magnitude. Performance evaluations measured relative illuminance, x chromaticity and y chromaticity shifts after each stress cycle. The Philips L Prize entry lamps appear to be appreciably more durable than the incumbent energy-efficient technology, as represented by the evaluated CFLs, and with respect to the applied stresses. Through the course of testing, all 15 CFL samples permanently ceased to function as a result of the applied stresses, while only 1 Philips L Prize entry lamp exhibited a failure, the nature of which was minor, non-destructive, and a consequence of a known (and resolved) subcontractor issue. Given that current CFL technology appears to be moderately mature and no Philips L Prize entry failures could be produced within the stress envelope causing 100 percent failure of the benchmark CFLs, it seems that, in this particular implementation, light-emitting diode (LED) technology would be much more durable in the field than current CFL technology. However, the Philips L Prize entry lamps used for testing were carefully designed and built for the competition, while the benchmark CFLs were mass produced for retail sale—a distinction that should be taken into consideration. Further reliability testing on final production samples would be necessary to judge the extent to which the results of this analysis apply to production versions

  17. FES cycling.

    Science.gov (United States)

    Newham, D J; Donaldson, N de N

    2007-01-01

    Spinal cord injury (SCI) leads to a partial or complete disruption of motor, sensory, and autonomic nerve pathways below the level of the lesion. In paraplegic patients, functional electrical stimulation (FES) was originally widely considered as a means to restore walking function but this was proved technically very difficult because of the numerous degrees of freedom involved in walking. FES cycling was developed for people with SCI and has the advantages that cycling can be maintained for reasonably long periods in trained muscles and the risk of falls is low. In the article, we review research findings relevant to the successful application of FES cycling including the effects on muscle size, strength and function, and the cardiovascular and bone changes. We also describe important practical considerations in FES cycling regarding the application of surface electrodes, training and setting up the stimulator limitations, implanted stimulators and FES cycling including FES cycling in groups and other FES exercises such as FES rowing.

  18. ELIMINATION AND IDENTITIES WITH THE INTEGRAL SIGN

    Institute of Scientific and Technical Information of China (English)

    Hongmei LIU; Tianming WANG

    2006-01-01

    In this paper, we aim at using the elimination in Weyl algebra to give an algorithm for proving the identities with integral sign. Then, we test several examples that have been proved by D Zeilberger.

  19. TOWARDS THE ELIMINATION OF PREVENTABLE DISEASES

    Directory of Open Access Journals (Sweden)

    O. V. Shamsheva

    2013-01-01

    Full Text Available The article presents incidence rates of major vaccine-preventable diseases in the world and the Russian Federation and cites mitigation measures that, in the end, must lead to the elimination of the diseases. 

  20. Integrated Entry Guidance for Reusable Launch Vehicle

    Institute of Scientific and Technical Information of China (English)

    NING Guo-dong; ZHANG Shu-guang; FANG Zhen-ping

    2007-01-01

    A method for the implementation of integrated three-degree-of-freedom constrained entry guidance for reusable launch vehicle is presented. Given any feasible entry conditions, terminal area energy management interface conditions, and the reference trajectory generated onboard then, the method can generate a longitudinal guidance profile rapidly, featuring linear quadratic regular method and a proportional-integral-derivative tracking law with time-varying gains, which satisfies all the entry corridor constraints and meets the requirements with high precision. Afterwards, by utilizing special features of crossrange parameter, establishing bank-reversal corridor,and determining bank-reversals according to dynamically adjusted method, the algorithm enables the lateral entry guidance system to fly a wide range of missions and provides reliable and good performance in the presence of significant aerodynamic modeling uncertainty.Fast trajectory guidance profiles and simulations with a reusable launch vehicle model for various missions and aerodynamic uncertainties are presented to demonstrate the capacity and reliability of this method.

  1. Border Crossing/Entry Data - Boarder Crossing

    Data.gov (United States)

    Department of Transportation — Border Crossing/Entry Data provides summary statistics for incoming crossings at the U.S.-Canadian and the U.S.-Mexican border at the port level. Data are available...

  2. Entry Mode and Performance of Nordic Firms

    DEFF Research Database (Denmark)

    Wulff, Jesper

    2015-01-01

    This study investigates whether the relationship between mode of international market entry and non-location bound international experience is weaker for firms that are large or have a high foreign to total sales ratio, labeled multinational experience. Empirical evidence based on 250 foreign...... market entries made by Norwegian, Danish and Swedish firms suggests that the association between equity mode choice and non-location bound international experience diminishes in the presence of higher levels of multinational experience. Furthermore, firms whose entry mode choice is predicted by the model...... including the proposed moderating effect, on average, yield higher post-entry performance. This study sheds light on inconsistent results found in previous research investigating the impact of international experience and has practical implications for managerial decision-making....

  3. The Effects of Entry in Bilateral Oligopoly

    Directory of Open Access Journals (Sweden)

    Alex Dickson

    2013-06-01

    Full Text Available The purpose of this paper is to study the effects of entry into the market for a single commodity in which both sellers and buyers are permitted to interact strategically. With the inclusion of an additional seller, the market is quasi-competitive: the price falls and volume of trade increases, as expected. However, contrary to the conventional wisdom, existing sellers’ payoffs may increase. The conditions under which entry by new sellers raises the equilibrium payoffs of existing sellers are derived. These depend in an intuitive way on the elasticity of a strategic analog of demand and the market share of existing sellers, and encompass entirely standard economic environments. Similar results are derived relating to the entry of additional buyers and the effects of entry on both sides of the market are investigated.

  4. Entry and exit decisions under uncertainty

    DEFF Research Database (Denmark)

    Kongsted, Hans Christian

    1996-01-01

    This paper establishes the general deterministic limit that corresponds to Dixit's model of entry and exit decisions under uncertainty. The interlinked nature of decisions is shown to be essential also in the deterministic limit. A numerical example illustrates the result......This paper establishes the general deterministic limit that corresponds to Dixit's model of entry and exit decisions under uncertainty. The interlinked nature of decisions is shown to be essential also in the deterministic limit. A numerical example illustrates the result...

  5. Hypersonic and planetary entry flight mechanics

    Science.gov (United States)

    Vinh, N. X.; Busemann, A.; Culp, R. D.

    1980-01-01

    The book treats hypersonic flight trajectories and atmospheric entry flight mechanics in light of their importance for space shuttle entry. Following a review of the structures of planetary atmospheres and aerodynamic forces, equations are derived for flight over a spherical planet, and the performance of long-range hypervelocity vehicles in extra-atmospheric flight is analyzed. Consideration is then given to vehicle trajectories in the powered and atmospheric reentry phases of flight, and several first-order solutions are derived for various planetary entry situations. The second-order theory of Loh for entry trajectories is presented along with the classical theories of Yaroshevskii and Chapman for entry into planetary atmospheres, and the thermal problems encountered in hypersonic flight are analyzed. A unified theory for entry into planetary atmospheres is then introduced which allows the performance of a general type of lifting vehicle to be studied, and applied to the analysis of orbit contraction due to atmospheric drag, flight with lift modulation and lateral maneuvers.

  6. Planetary/DOD entry technology flight experiments. Volume 2: Planetary entry flight experiments

    Science.gov (United States)

    Christensen, H. E.; Krieger, R. J.; Mcneilly, W. R.; Vetter, H. C.

    1976-01-01

    The technical feasibility of launching a high speed, earth entry vehicle from the space shuttle to advance technology for the exploration of the outer planets' atmospheres was established. Disciplines of thermodynamics, orbital mechanics, aerodynamics propulsion, structures, design, electronics and system integration focused on the goal of producing outer planet environments on a probe shaped vehicle during an earth entry. Major aspects of analysis and vehicle design studied include: planetary environments, earth entry environment capability, mission maneuvers, capabilities of shuttle upper stages, a comparison of earth entry planetary environments, experiment design and vehicle design.

  7. Martian Atmospheric Pressure Static Charge Elimination Tool

    Science.gov (United States)

    Johansen, Michael R.

    2014-01-01

    A Martian pressure static charge elimination tool is currently in development in the Electrostatics and Surface Physics Laboratory (ESPL) at NASA's Kennedy Space Center. In standard Earth atmosphere conditions, static charge can be neutralized from an insulating surface using air ionizers. These air ionizers generate ions through corona breakdown. The Martian atmosphere is 7 Torr of mostly carbon dioxide, which makes it inherently difficult to use similar methods as those used for standard atmosphere static elimination tools. An initial prototype has been developed to show feasibility of static charge elimination at low pressure, using corona discharge. A needle point and thin wire loop are used as the corona generating electrodes. A photo of the test apparatus is shown below. Positive and negative high voltage pulses are sent to the needle point. This creates positive and negative ions that can be used for static charge neutralization. In a preliminary test, a floating metal plate was charged to approximately 600 volts under Martian atmospheric conditions. The static elimination tool was enabled and the voltage on the metal plate dropped rapidly to -100 volts. This test data is displayed below. Optimization is necessary to improve the electrostatic balance of the static elimination tool.

  8. 19 CFR 142.44 - Entry number range.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry number range. 142.44 Section 142.44 Customs... (CONTINUED) ENTRY PROCESS Line Release § 142.44 Entry number range. After an application for Line Release has received final approval, filers must provide the port director, in writing, with a range of entry...

  9. 77 FR 5681 - Establishment of Global Entry Program

    Science.gov (United States)

    2012-02-06

    ... assist in developing a marketing strategy. The firm has created a new Global Entry logo and tag line... SENTRI II. Analysis of Comments A. Program Performance and Entry Process B. Marketing of Global Entry C.... Marketing of Global Entry Comment: CBP received two comments suggesting that CBP should expand marketing...

  10. Temporal analysis of hepatitis C virus cell entry with occludin directed blocking antibodies.

    Directory of Open Access Journals (Sweden)

    Marion Sourisseau

    2013-03-01

    Full Text Available Hepatitis C virus (HCV is a major cause of liver disease worldwide. A better understanding of its life cycle, including the process of host cell entry, is important for the development of HCV therapies and model systems. Based on the requirement for numerous host factors, including the two tight junction proteins claudin-1 (CLDN1 and occludin (OCLN, HCV cell entry has been proposed to be a multi-step process. The lack of OCLN-specific inhibitors has prevented a comprehensive analysis of this process. To study the role of OCLN in HCV cell entry, we created OCLN mutants whose HCV cell entry activities could be inhibited by antibodies. These mutants were expressed in polarized HepG2 cells engineered to support the complete HCV life cycle by CD81 and miR-122 expression and synchronized infection assays were performed to define the kinetics of HCV cell entry. During these studies, OCLN utilization differences between HCV isolates were observed, supporting a model that HCV directly interacts with OCLN. In HepG2 cells, both HCV cell entry and tight junction formation were impaired by OCLN silencing and restored by expression of antibody regulatable OCLN mutant. Synchronized infection assays showed that glycosaminoglycans and SR-BI mediated host cell binding, while CD81, CLDN1 and OCLN all acted sequentially at a post-binding stage prior to endosomal acidification. These results fit a model where the tight junction region is the last to be encountered by the virion prior to internalization.

  11. Has Wild Poliovirus Been Eliminated from Nigeria?

    Science.gov (United States)

    Famulare, Michael

    2015-01-01

    Wild poliovirus type 3 (WPV3) has not been seen anywhere since the last case of WPV3-associated paralysis in Nigeria in November 2012. At the time of writing, the most recent case of wild poliovirus type 1 (WPV1) in Nigeria occurred in July 2014, and WPV1 has not been seen in Africa since a case in Somalia in August 2014. No cases associated with circulating vaccine-derived type 2 poliovirus (cVDPV2) have been detected in Nigeria since November 2014. Has WPV1 been eliminated from Africa? Has WPV3 been eradicated globally? Has Nigeria interrupted cVDPV2 transmission? These questions are difficult because polio surveillance is based on paralysis and paralysis only occurs in a small fraction of infections. This report provides estimates for the probabilities of poliovirus elimination in Nigeria given available data as of March 31, 2015. It is based on a model of disease transmission that is built from historical polio incidence rates and is designed to represent the uncertainties in transmission dynamics and poliovirus detection that are fundamental to interpreting long time periods without cases. The model estimates that, as of March 31, 2015, the probability of WPV1 elimination in Nigeria is 84%, and that if WPV1 has not been eliminated, a new case will be detected with 99% probability by the end of 2015. The probability of WPV3 elimination (and thus global eradication) is > 99%. However, it is unlikely that the ongoing transmission of cVDPV2 has been interrupted; the probability of cVDPV2 elimination rises to 83% if no new cases are detected by April 2016.

  12. Lessons learned from planetary entry probe missions

    Science.gov (United States)

    Niemann, Hasso; Atreya, Sushil K.; Kasprzak, Wayne

    Probing the atmospheres and surfaces of the planets and their moons with fast moving entry probes has been a very useful and essential technique to obtain in situ or quasi in situ scientific data (ground truth) which could not otherwise be obtained from fly by or orbiter only missions and where balloon, aircraft or lander missions are too complex and too costly. Planetary entry probe missions have been conducted successfully on Venus, Mars, Jupiter and Titan after having been first demonstrated in the Earth's atmosphere. Future planetary missions should also include more entry probe missions back to Venus and to the outer planets. The success of and science returns from past missions, the need for more and unique data, and a continuously advancing technology generate confidence that future missions will be even more successful with respect to science return and technical performance. There are, however, unique challenges associated with entry probe missions and with building instruments for an entry probe, as compared to orbiters, landers, or rovers. Conditions during atmospheric entry are extreme. There are operating time constraints due to the usually short duration of the probe descent, and the instruments experience rapid environmental changes in temperature and pressure. In addition, there are resource limitations, i.e. mass, power, size and bandwidth. Because of the protective heat shield and the high acceleration the probe experiences during entry, the ratio of payload to total probe mass is usually much smaller than in other missions. Finally, the demands on the instrument design are determined in large part by conditions (pressure, temperature, composition) unique to the particular body under study, and as a result, there is no one-size-fits-all instrument for an atmospheric probe. Many of these requirements are more easily met by miniaturizing the probe instrumentation and consequently reducing the required size of the probe. Improved heat shield

  13. 76 FR 82315 - Agency Information Collection Activities: Entry/Immediate Delivery Application and Simplified Entry

    Science.gov (United States)

    2011-12-30

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Entry/Immediate... Budget (OMB) for review and approval in accordance with the Paperwork Reduction Act: Entry/Immediate... process is conducted in accordance with 5 CFR 1320.10. DATES: Written comments should be received on or...

  14. Eliminating Late Recurrence to Eradicate Breast Cancer

    Science.gov (United States)

    2015-09-01

    CoA, which enter the citric acid cycle , and NADH and FADH2. in neonatal Atg5−/− mice, indicative of systemic ATP depletion. Because the metabolic...multiple defects in mitochondrial metabolism, including decreased production of tricarboxylic acid (TCA) cycle intermediates, reduced mitochondrial... cycle for energy production or used as substrates for glucose production by gluconeogenesis. b | Fatty acids produced via lipophagy are converted

  15. Noise elimination algorithm for modal analysis

    Energy Technology Data Exchange (ETDEWEB)

    Bao, X. X., E-mail: baoxingxian@upc.edu.cn [Department of Naval Architecture and Ocean Engineering, China University of Petroleum (East China), Qingdao 266580 (China); Li, C. L. [Key Laboratory of Marine Geology and Environment, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 (China); Xiong, C. B. [The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061 (China)

    2015-07-27

    Modal analysis is an ongoing interdisciplinary physical issue. Modal parameters estimation is applied to determine the dynamic characteristics of structures under vibration excitation. Modal analysis is more challenging for the measured vibration response signals are contaminated with noise. This study develops a mathematical algorithm of structured low rank approximation combined with the complex exponential method to estimate the modal parameters. Physical experiments using a steel cantilever beam with ten accelerometers mounted, excited by an impulse load, demonstrate that this method can significantly eliminate noise from measured signals and accurately identify the modal frequencies and damping ratios. This study provides a fundamental mechanism of noise elimination using structured low rank approximation in physical fields.

  16. Recognizing, Confronting, and Eliminating Workplace Bullying.

    Science.gov (United States)

    Berry, Peggy Ann; Gillespie, Gordon L; Fisher, Bonnie S; Gormley, Denise K

    2016-07-01

    Workplace bullying (WPB) behaviors negatively affect nurse productivity, satisfaction, and retention, and hinder safe patient care. The purpose of this article is to define WPB, differentiate between incivility and WPB, and recommend actions to prevent WPB behaviors. Informed occupational and environmental health nurses and nurse leaders must recognize, confront, and eliminate WPB in their facilities and organizations. Recognizing, confronting, and eliminating WPB behaviors in health care is a crucial first step toward sustained improvements in patient care quality and the health and safety of health care employees. © 2016 The Author(s).

  17. Elimination and recursions in the scattering equations

    Directory of Open Access Journals (Sweden)

    Carlos Cardona

    2016-05-01

    Full Text Available We use the elimination theory to explicitly construct the (n−3! order polynomial in one of the variables of the scattering equations. The answer can be given either in terms of a determinant of Sylvester type of dimension (n−3! or a determinant of Bézout type of dimension (n−4!. We present a recursive formula for the Sylvester determinant. Expansion of the determinants yields expressions in terms of Plücker coordinates. Elimination of the rest of the variables of the scattering equations is also presented.

  18. Ten years left to eliminate blinding trachoma

    Directory of Open Access Journals (Sweden)

    Haddad D.

    2010-09-01

    Full Text Available n 1997, the World Health Organization formed the Global Alliance to Eliminate Blinding Trachoma by 2020 (GET 2020, a coalition of governmental, non-governmental, research, and pharmaceutical partners. In 1998, the World Health Assembly urged member states to map blinding trachoma in endemic areas, implement the SAFE strategy (which stands for surgery for trichiasis, antibiotics, facial-cleanliness and environmental change, such as clean water and latrines and collaborate with the global alliance in its work to eliminate blinding trachoma.

  19. Dynamics of Hydrogen Elimination from Unsaturated Hydrocarbons

    Science.gov (United States)

    Cromwell, Evan Francis

    State-of-the-art laser and molecular beam techniques are used to study the dynamics of H_2 elimination from 1,4-cyclohexadiene and ethylene. Information on the transition state configurations and the dynamics of the dissociation processes for these reactions is reported. A brief introduction to these studies is presented in Chapter I. An ultrahigh-brightness laser system, developed to study the spectroscopy and dynamics of molecules and clusters from the vacuum ultraviolet (VUV) and extreme ultraviolet (XUV) to the near infra-red spectral regions is described in Chapter II. The laser utilizes pulse amplification of a single-mode ring dye laser, frequency doubling, and four-wave mixing in a pulsed jet. Pulse energies of > 120mJ in the visible, >35mJ in the uv, >15mJ in the infra-red and > 10^{11} photons/pulse in the VUV-XUV have been obtained. The bandwidth of the laser has been measured to be 91MHz in the visible and 210MHz in the XUV. In Chapter III a comprehensive study of the dynamics of H_2 elimination from 1,4- and 1,3-cyclohexadiene is reported. Rotational and vibrational quantum state distributions as well as translation energy distributions for the H_2 product are measured. State specific detection of H_2 is accomplished with a transform limited vuv-xuv laser system via (1 + 1) REMPI. Rate constants for the H_2 elimination and 1,4 to 1,3 isomerization reactions are derived. A (v, J) correlation for H _2 with {bf v}| {bf J} primarily is observed from anisotropy in the Doppler profiles. A clear picture of the transition state configuration of 1,4-cyclohexadiene is provided from the information obtained. A study of the dynamics of H_2 elimination from ethylene is presented in the last Chapter. A complete H_2 product energy distribution is obtained. The distribution of energy in H_2 product from the dissociation of 1,1-d_2 ethylene is also presented. Two separate H_2 elimination channels are observed, a 1,1 elimination and a 1,2 elimination, in the

  20. Autonomous gliding entry guidance with geographic constraints

    Institute of Scientific and Technical Information of China (English)

    Guo Jie; Wu Xuzhong; Tang Shengjing

    2015-01-01

    This paper presents a novel three-dimensional autonomous entry guidance for relatively high lift-to-drag ratio vehicles satisfying geographic constraints and other path constraints. The guidance is composed of onboard trajectory planning and robust trajectory tracking. For trajectory planning, a longitudinal sub-planner is introduced to generate a feasible drag-versus-energy profile by using the interpolation between upper boundary and lower boundary of entry corridor to get the desired trajectory length. The associated magnitude of the bank angle can be specified by drag profile, while the sign of bank angle is determined by lateral sub-planner. Two-reverse mode is utilized to satisfy waypoint constraints and dynamic heading error corridor is utilized to satisfy no-fly zone constraints. The longitudinal and lateral sub-planners are iteratively employed until all of the path constraints are satisfied. For trajectory tracking, a novel tracking law based on the active disturbance rejection control is introduced. Finally, adaptability tests and Monte Carlo simulations of the entry guidance approach are performed. Results show that the proposed entry guidance approach can adapt to different entry missions and is able to make the vehicle reach the prescribed target point precisely in spite of geographic constraints.

  1. Financial Performance of Entry Mode Decisions

    DEFF Research Database (Denmark)

    Boyd, Britta; Dyhr Ulrich, Anna Marie; Hollensen, Svend

    2012-01-01

    Based on a survey of 170 Danish SMEs the paper examines influences on entry mode choices and the financial outcome of these decisions. The main research objectives are divided into two steps: Step 1: To determine the factors influencing the choice of foreign entry modes by Danish companies. Step ...... and implications are provided for companies willing to invest more into foreign markets in order to achieve a higher degree of control and better financial results.......Based on a survey of 170 Danish SMEs the paper examines influences on entry mode choices and the financial outcome of these decisions. The main research objectives are divided into two steps: Step 1: To determine the factors influencing the choice of foreign entry modes by Danish companies. Step 2......: To determine the relationship between the choice of entry mode and export performance, measured in terms of financial outcome. Drawing from transaction cost theory the authors develop and test a model where different factors affect the level of control chosen by the parent company. This study contributes...

  2. Shuttle program. MCC Level C formulation requirements: Entry guidance and entry autopilot

    Science.gov (United States)

    Harpold, J. C.; Hill, O.

    1980-01-01

    A set of preliminary entry guidance and autopilot software formulations is presented for use in the Mission Control Center (MCC) entry processor. These software formulations meet all level B requirements. Revision 2 incorporates the modifications required to functionally simulate optimal TAEM targeting capability (OTT). Implementation of this logic in the MCC must be coordinated with flight software OTT implementation and MCC TAEM guidance OTT. The entry guidance logic is based on the Orbiter avionics entry guidance software. This MCC requirements document contains a definition of coordinate systems, a list of parameter definitions for the software formulations, a description of the entry guidance detailed formulation requirements, a description of the detailed autopilot formulation requirements, a description of the targeting routine, and a set of formulation flow charts.

  3. Fes cycling

    Directory of Open Access Journals (Sweden)

    Berkelmans Rik

    2008-01-01

    Full Text Available Many research with functional electrical stimulation (FES has been done to regain mobility and for health benefits. Better results have been reported for FES-cycling than for FES-walking. The majority of the subjects during such research are people with a spinal cord injury (SCI, cause they often lost skin sensation. Besides using surface stimulation also implanted stimulators can be used. This solves the skin sensation problem, but needs a surgery. Many physiological effects of FES-cycling has been reported, e.g., increase of muscles, better blood flow, reduction of pressure ulcers, improved self-image and some reduction of bone mineral density (BMD loss. Also people with an incomplete SCI benefit by FES-cycling, e.g. cycling time without FES, muscle strength and also the walking abilities increased. Hybrid exercise gives an even better cardiovascular training. Presently 4 companies are involved in FES-cycling. They all have a stationary mobility trainer. Two of them also use an outdoor tricycle. One combined with voluntary arm cranking. By optimizing the stimulation parameters the power output and fatigue resistance will increase, but will still be less compared to voluntary cycling.

  4. Binding of more than one Tva800 molecule is required for ASLV-A entry

    Directory of Open Access Journals (Sweden)

    Gray Eleanor R

    2011-11-01

    Full Text Available Abstract Background Understanding the mechanism by which viruses enter their target cell is an essential part of understanding their infectious cycle. Previous studies have focussed on the multiplicity of viral envelope proteins that need to bind to their cognate receptor to initiate entry. Avian sarcoma and leukosis virus Envelope protein (ASLV Env mediates entry via a receptor, Tva, which can be attached to the cell surface either by a phospholipid anchor (Tva800 or a transmembrane domain (Tva950. In these studies, we have now investigated the number of target receptors necessary for entry of ASLV Env-pseudotyped virions. Results Using titration and modelling experiments we provide evidence that binding of more than one receptor, probably two, is needed for entry of virions via Tva800. However, binding of just one Tva950 receptor is sufficient for successful entry. Conclusions The different modes of attachment of Tva800 and Tva950 to the cell membrane have important implications for the utilisation of these proteins as receptors for viral binding and/or uptake.

  5. Biomechanics of Counterweighted One-Legged Cycling.

    Science.gov (United States)

    Elmer, Steven J; McDaniel, John; Martin, James C

    2016-02-01

    One-legged cycling has served as a valuable research tool and as a training and rehabilitation modality. Biomechanics of one-legged cycling are unnatural because the individual must actively lift the leg during flexion, which can be difficult to coordinate and cause premature fatigue. We compared ankle, knee, and hip biomechanics between two-legged, one-legged, and counterweighted (11.64 kg) one-legged cycling. Ten cyclists performed two-legged (240 W), one-legged (120 W), and counterweighted one-legged (120 W) cycling (80 rpm). Pedal forces and limb kinematics were recorded to determine work during extension and flexion. During counterweighted one-legged cycling relative ankle dorsiflexion, knee flexion, and hip flexion work were less than one-legged but greater than two-legged cycling (all P cycling were greater than one-legged but less than two-legged cycling (all P cycling reduced but did not eliminate differences in joint flexion and extension actions between one- and two-legged cycling. Even with these differences, counterweighted one-legged cycling seemed to have advantages over one-legged cycling. These results, along with previous work highlighting physiological characteristics and training adaptations to counterweighted one-legged cycling, demonstrate that this exercise is a viable alternative to one-legged cycling.

  6. Mechanism of store-operated calcium entry

    Indian Academy of Sciences (India)

    Devkanya Dutta

    2000-12-01

    Activation of receptors coupled to the phospholipase C/IP3 signalling pathway results in a rapid release of calcium from its intracellular stores, eventually leading to depletion of these stores. Calcium store depletion triggers an influx of extracellular calcium across the plasma membrane, a mechanism known as the store-operated calcium entry or capacitative calcium entry. Capacitative calcium current plays a key role in replenishing calcium stores and activating various physiological processes. Despite considerable efforts, very little is known about the molecular nature of the capacitative channel and the signalling pathway that activates it. This review summarizes our current knowledge about store operated calcium entry and suggests possible hypotheses for its mode of activation.

  7. Modes and orders of market entry

    DEFF Research Database (Denmark)

    Ulhøi, John Parm

    2012-01-01

    This paper focuses on the initial questions of how and when to enter a market from the perspective of a firm. By entry mode is meant a firm’s strategy (innovation or imitation) for entering the market in response to environmental changes. Entry order refers to the related issue of market timing...... strategies are more ambiguous. Based on a corporate technology and innovation strategy perspective, the paper reconceptualises and extends existing modes and orders of market entry, and in particular clarifies the ambiguity associated with imitative strategies. Four distinct imitator strategies...... are identified. The paper closes with a brief discussion of limitations, avenues for future research, and implications for managers and affected policymakers....

  8. Entry, Descent, and Landing for Human Mars Missions

    Science.gov (United States)

    Munk, Michelle M.; DwyerCianciolo, Alicia M.

    2012-01-01

    One of the most challenging aspects of a human mission to Mars is landing safely on the Martian surface. Mars has such low atmospheric density that decelerating large masses (tens of metric tons) requires methods that have not yet been demonstrated, and are not yet planned in future Mars missions. To identify the most promising options for Mars entry, descent, and landing, and to plan development of the needed technologies, NASA's Human Architecture Team (HAT) has refined candidate methods for emplacing needed elements of the human Mars exploration architecture (such as ascent vehicles and habitats) on the Mars surface. This paper explains the detailed, optimized simulations that have been developed to define the mass needed at Mars arrival to accomplish the entry, descent, and landing functions. Based on previous work, technology options for hypersonic deceleration include rigid, mid-L/D (lift-to-drag ratio) aeroshells, and inflatable aerodynamic decelerators (IADs). The hypersonic IADs, or HIADs, are about 20% less massive than the rigid vehicles, but both have their technology development challenges. For the supersonic regime, supersonic retropropulsion (SRP) is an attractive option, since a propulsive stage must be carried for terminal descent and can be ignited at higher speeds. The use of SRP eliminates the need for an additional deceleration system, but SRP is at a low Technology Readiness Level (TRL) in that the interacting plumes are not well-characterized, and their effect on vehicle stability has not been studied, to date. These architecture-level assessments have been used to define the key performance parameters and a technology development strategy for achieving the challenging mission of landing large payloads on Mars.

  9. 102 Women Win Illiteracy Elimination Awards

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    SHEN Kexin is one of the 102 women who won the Women’s Illiteracy Elimination Award (WIEA) in 1995. Through her unremitting efforts, 326 illiterate women learned to read and write and 44 girls went back to school. The award winners came from different ethnic groups and different walks of life. Thirty local governments and women’s organizations also won the award.

  10. Teaching Projectile Motion to Eliminate Misconceptions

    Science.gov (United States)

    Prescott, Anne; Mitchelmore, Michael

    2005-01-01

    Student misconceptions of projectile motion are well documented, but their effect on the teaching and learning of the mathematics of motion under gravity has not been investigated. An experimental unit was designed that was intended to confront and eliminate misconceptions in senior secondary school students. The approach was found to be…

  11. Eliminating transducer distortion in acoustic measurements

    DEFF Research Database (Denmark)

    Agerkvist, Finn T.; Torras Rosell, Antoni; McWalter, Richard Ian

    2014-01-01

    This paper investigates the in uence of nonlinear components that contaminate the linear response of acoustic transducer, and presents a method for eliminating the in uence of nonlinearities in acoustic measurements. The method is evaluated on simulated as well as experimental data, and is shown...

  12. Gram-Schmidt Orthogonalization by Gauss Elimination.

    Science.gov (United States)

    Pursell, Lyle; Trimble, S. Y.

    1991-01-01

    Described is the hand-calculation method for the orthogonalization of a given set of vectors through the integration of Gaussian elimination with existing algorithms. Although not numerically preferable, this method adds increased precision as well as organization to the solution process. (JJK)

  13. Eliminating Problems Caused by Multicollinearity: A Warning.

    Science.gov (United States)

    Kennedy, Peter E.

    1982-01-01

    Explains why an econometric practice introduced by J.C. Soper cannot eliminate the problems caused by multicollinearity. The author suggests that it can be a useful technique in that it forces researchers to pay more attention to the specifications of their models. (AM)

  14. Automated Re-Entry System using FNPEG

    Science.gov (United States)

    Johnson, Wyatt R.; Lu, Ping; Stachowiak, Susan J.

    2017-01-01

    This paper discusses the implementation and simulated performance of the FNPEG (Fully Numerical Predictor-corrector Entry Guidance) algorithm into GNC FSW (Guidance, Navigation, and Control Flight Software) for use in an autonomous re-entry vehicle. A few modifications to FNPEG are discussed that result in computational savings -- a change to the state propagator, and a modification to cross-range lateral logic. Finally, some Monte Carlo results are presented using a representative vehicle in both a high-fidelity 6-DOF (degree-of-freedom) sim as well as in a 3-DOF sim for independent validation.

  15. Form, its meaning, and dictionary entries

    Directory of Open Access Journals (Sweden)

    Violetta Koseska-Toszewa

    2015-11-01

    It is worth stressing that distinguishing between the form and its meaning in comparing the material 6 languages belonging to three different groups of Slavic languages (as is the case in the MONDILEX Project will allow us to avoid numeorus substantiva mistakes and erroneous conclusions. Hence dictionary entries should be verified and made uniform in that respect before they are “digitalized”... Distinction between the form and its meaning in a dictionary entry is fully possible, as shown by works of Z. Saloni (2002 and A.Przepiórkowski (2008.

  16. Project Prometheus and Future Entry Probe Missions

    Science.gov (United States)

    Spilker, Thomas R.

    2005-01-01

    A viewgraph presentation on project Prometheus and future entry probe missions is shown. The topics include: 1) What Is Project Prometheus?; 2) What Capabilities Can Project Prometheus Offer? What Mission Types Are Being Considered?; 3) Jupiter Icy Moons Orbiter (JIMO); 4) How Are Mission Opportunities Changing?; 5) Missions Of Interest a Year Ago; 6) Missions Now Being Considered For Further Study; 7) Galileo-Style (Conventional) Probe Delivery; 8) Galileo-Style Probe Support; 9) Conventional Delivery and Support of Multiple Probes; 10) How Entry Probe Delivery From an NEP Vehicle Is Different; and 11) Concluding Remarks.

  17. "Generic Entry and the Pricing of Pharmaceuticals"

    OpenAIRE

    Frank, Richard G.; David S. Salkever

    1995-01-01

    During the 1980s the share of prescriptions sold by retail pharmacies that was accounted for by generic products roughly doubled. The price response to generic entry of brand-name products has been a source of controversy. In this paper we estimate models of price responses to generic entry in the market for brand-name and generic drugs. We study a sample of 32 drugs that lost patent protection during the early to mid-1980s. Our results provide strong evidence that brand-name prices increase ...

  18. Eliminating schistosomes through vaccination: What are the best immune weapons?

    Directory of Open Access Journals (Sweden)

    Cristina Toscano Fonseca

    2015-03-01

    Full Text Available The successful development of vaccines depend on the knowledge of the immunological mechanisms associated with the elimination of the pathogen. In the case of schistosomes, its complex life cycle and the mechanisms developed to evade host immune system, turns the development of a vaccine against the disease into a very difficult task. Identifying the immunological effector mechanisms involved in parasite attrition and the major targets for its response is a key step to formulate an effective vaccine. Recent studies have described some promising antigens to compose a subunit vaccine and have pointed to some immune factors that play a role in parasite elimination. Here we review the immune components and effector mechanisms associated with the protective immunity induced by those vaccine candidates and the lessons we have learned from the studies of the acquired resistance to infection in humans. We will also discuss the immune factors that correlate with protection and therefore could help to evaluate those vaccine formulations in clinical trials.

  19. Renal lactate elimination is maintained during moderate exercise in humans

    DEFF Research Database (Denmark)

    Volianitis, Stefanos; Dawson, Ellen A.; Dalsgaard, Mads

    2012-01-01

    was determined in nine men during sodium lactate infusion to enhance the evaluation (0.5 mol x L(-1) at 16 ± 1 mL x min(-1); mean ± s) both at rest and during cycling exercise (heart rate 139 ± 5 beats x min(-1)). The renal release of erythropoietin was used to detect kidney tissue ischaemia. At rest, the a-v O......(2) (CaO(2)-CvO(2)) and lactate concentration differences were 0.8 ± 0.2 and 0.02 ± 0.02 mmol x L(-1), respectively. During exercise, arterial lactate and CaO(2)-CvO(2) increased to 7.1 ± 1.1 and 2.6 ± 0.8 mmol x L(-1), respectively (P ...Reduced hepatic lactate elimination initiates blood lactate accumulation during incremental exercise. In this study, we wished to determine whether renal lactate elimination contributes to the initiation of blood lactate accumulation. The renal arterial-to-venous (a-v) lactate difference...

  20. Global Promoter Targeting of a Conserved Lysine Deacetylase for Transcriptional Shutoff during Quiescence Entry.

    Science.gov (United States)

    McKnight, Jeffrey N; Boerma, Joseph W; Breeden, Linda L; Tsukiyama, Toshio

    2015-09-01

    Quiescence is a conserved cell-cycle state characterized by cell-cycle arrest, increased stress resistance, enhanced longevity, and decreased transcriptional, translational, and metabolic output. Although quiescence plays essential roles in cell survival and normal differentiation, the molecular mechanisms leading to this state are not well understood. Here, we determined changes in the transcriptome and chromatin structure of S. cerevisiae upon quiescence entry. Our analyses revealed transcriptional shutoff that is far more robust than previously believed and an unprecedented global chromatin transition, which are tightly correlated. These changes require Rpd3 lysine deacetylase targeting to at least half of gene promoters via quiescence-specific transcription factors including Xbp1 and Stb3. Deletion of RPD3 prevents cells from establishing transcriptional quiescence, leading to defects in quiescence entry and shortening of chronological lifespan. Our results define a molecular mechanism for global reprogramming of transcriptome and chromatin structure for quiescence driven by a highly conserved chromatin regulator.

  1. Position-optimization on retained entry and backfilling wall in gob-side entry retaining techniques

    Institute of Scientific and Technical Information of China (English)

    Xiaowei Feng; Nong Zhang

    2015-01-01

    This study investigates the stability problem of gob-side entry retaining (GER) and backfilling wall which located under the key block B. Based on the combined research of elastic–plastic mechanics, structure mechanics and modern theory of mining-induced pressure, the caving characteristic and roof structure over the GER were analyzed, and the vertical force and the torque on retained entry roof were also derived as the position for the retained entry varies. On the basis of the specific geology in Huainan mining area, the results indicate that a relatively more stable position for retained entry neighbors the hinge point of block A and B, and it also located at a scope ranging from this point to the one-third length of block B in horizontal direction. As to appropriate position for backfilling wall, this study recommends partial-road-in backfilling method for GER. Field trial conducted at panel face 12418 of Xieqiao Mine demonstrates that the recommended width for original entry is 3.6 m and the preferred width proportion between original retained entry and original entry is 75%or so whereas the avoidable one is 88%or so. These findings provide qualitative references to the mines which share similar geology as what Huainan mining area characterized.

  2. Nuclear energy selected entries from the encyclopedia of sustainability science and technology

    CERN Document Server

    Tsoulfanidis, Nicholas

    2012-01-01

    Nuclear Energy provides an authoritative reference on all aspects of the nuclear industry from fundamental reactor physics calculations to reactor design, nuclear fuel resources, nuclear fuel cycle, radiation detection and protection, and nuclear power economics.  Featuring 19 peer-reviewed entries by recognized authorities in the field, this book provides comprehensive, streamlined coverage of fundamentals, current areas of research, and goals for the future.  The chapters will appeal to undergraduate and graduate students, researchers, and energy industry experts.

  3. Glacial cycles

    DEFF Research Database (Denmark)

    Kaufmann, R. K.; Juselius, Katarina

    We use a statistical model, the cointegrated vector autoregressive model, to assess the degree to which variations in Earth's orbit and endogenous climate dynamics can be used to simulate glacial cycles during the late Quaternary (390 kyr-present). To do so, we estimate models of varying complexity...... and compare the accuracy of their in-sample simulations. Results indicate that strong statistical associations between endogenous climate variables are not enough for statistical models to reproduce glacial cycles. Rather, changes in solar insolation associated with changes in Earth's orbit are needed...

  4. Open cycle thermoacoustics

    Energy Technology Data Exchange (ETDEWEB)

    Reid, Robert Stowers [Georgia Inst. of Technology, Atlanta, GA (United States)

    2000-01-01

    A new type of thermodynamic device combining a thermodynamic cycle with the externally applied steady flow of an open thermodynamic process is discussed and experimentally demonstrated. The gas flowing through this device can be heated or cooled in a series of semi-open cyclic steps. The combination of open and cyclic flows makes possible the elimination of some or all of the heat exchangers (with their associated irreversibility). Heat is directly exchanged with the process fluid as it flows through the device when operating as a refrigerator, producing a staging effect that tends to increase First Law thermodynamic efficiency. An open-flow thermoacoustic refrigerator was built to demonstrate this concept. Several approaches are presented that describe the physical characteristics of this device. Tests have been conducted on this refrigerator with good agreement with a proposed theory.

  5. 19 CFR 4.8 - Preliminary entry.

    Science.gov (United States)

    2010-04-01

    ... electronic equivalent of a complete Customs Form 1302 (Cargo Declaration), in the manner provided in § 4.7(b... 19 Customs Duties 1 2010-04-01 2010-04-01 false Preliminary entry. 4.8 Section 4.8 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE...

  6. 19 CFR 145.25 - Entry correct.

    Science.gov (United States)

    2010-04-01

    ... director believes the duty originally assessed was correct, he shall send the addressee a notice in writing... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry correct. 145.25 Section 145.25 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE...

  7. Continuous Aerodynamic Modelling of Entry Shapes

    NARCIS (Netherlands)

    Dirkx, D.; Mooij, E.

    2011-01-01

    During the conceptual design phase of a re-entry vehicle, the vehicle shape can be varied and its impact on performance evaluated. To this end, the continuous modeling of the aerodynamic characteristics as a function of the shape is useful in exploring the full design space. Local inclination method

  8. Radon entry into a simple test structure

    DEFF Research Database (Denmark)

    Andersen, C.E.; Søgaard-Hansen, J.; Majborn, B.

    1992-01-01

    A simple test structure for studies of radon entry into houses has been constructed at a field site at Riso National Laboratory. It consists of a 40 1, stainless-steel cylinder placed in a 0.52 m deep quadratic excavation with a side length of 2.4 m. The excavation is lined with an airtight...

  9. Flavivirus cell entry and membrane fusion

    NARCIS (Netherlands)

    Smit, Jolanda M.; Moesker, Bastiaan; Rodenhuis-Zybert, Izabela; Wilschut, Jan

    2011-01-01

    Flaviviruses, such as dengue virus and West Nile virus, are enveloped viruses that infect cells through receptor-mediated endocytosis and fusion from within acidic endosomes. The cell entry process of flaviviruses is mediated by the viral E glycoprotein. This short review will address recent advance

  10. Processes with multiple entries and exits

    NARCIS (Netherlands)

    Bergstra, J.A.; Stefanescu, G.

    1995-01-01

    This paper is an attempt to integrate the algebra of communicating processes (ACP) and the algebra of flownomials (AF). Basically, this means to combine axiomatized parallel and looping operators. To this end we introduce a model of process graphs with multiple entries and exits. In this model the u

  11. HIV-1 Entry Inhbitors: An Overview

    Science.gov (United States)

    Kuritzkes, Daniel R.

    2009-01-01

    Purpose of review This review provides an overview of HIV-1 entry inhibitors, with a focus on chemokine receptor antagonists. Recent findings Entry of HIV-1 into target cells is an ordered multi-step process involving attachment, co-receptor binding and fusion. Inhibitors of each step have been identified and shown to have antiviral activity in clinical trials. Phase 1-2 trials of monoclonal antibodies and small-molecule attachment inhibitors have demonstrated activity in HIV-1-infected subjects, but none has progressed to later phase clinical trials. The post-attachment inhibitor ibalizumab has shown activity in phase 1 and 2 trials; further studies are anticipated. The CCR5 antagonists maraviroc (now been approved for clinical use) and vicriviroc (in phase 3 trials) have shown significant benefit in controlled trials in treatment-experienced subjects; additional CCR5 antagonists are in various stages of clinical development. Targeting CXCR4 has proven to be more challenging. Although proof of concept has been demonstrated in phase 1-2 trials of two compounds, neither proved suitable for chronic administration. Little progress has been reported in developing longer acting or orally bioavailable fusion inhibitors. Summary ACCR5 antagonist and a fusion inhibitor are approved for use as HIV-1 entry inhibitors. Development of drugs targeting other steps in HIV-1 entry is ongoing. PMID:19339945

  12. Duopoly Dynamics with a Barrier to Entry

    NARCIS (Netherlands)

    Abbring, J.H.; Campbell, Jeffrey R.

    2007-01-01

    This paper considers the effects of raising the cost of entry for a potential competitor on infinite-horizon Markov-perfect duopoly dynamics with ongoing demand uncertainty. All entrants serving the model industry incur sunk costs, and exit avoids future fixed costs. We focus on the unique equilibri

  13. Entry modes of European firms in Vietnam

    Directory of Open Access Journals (Sweden)

    Daniel Simonet

    2012-09-01

    Full Text Available Purpose: The purpose of the paper is to explore the entry modes of EU firms setting up operations in Vietnam. Design/methodology/approach: we use a case study approach on Haymarket, Cadbury, Creative Education, Fairchild, Aventis and Artemisinin and Farming International using interviews from managerial professionals in Vietnam. Findings: Despite the fact that Vietnam has been opening up for more than 20 years, licensing is the preferred entry mode because of the risks involved in venturing with local firms; that preference signals a low level commitment and a high perception of risk and state interference. In line with Vietnam transition to state - rather than private market - capitalism, a foreign company opting for a joint-venture will do so with a state-owned rather than privately-owned company. The choice of a subsidiary can be explained by the lack of trust in partners and institutions, not by improvement in the socio-political environment. Limitations: In determining the entry mode strategy, the paper focuses on the Uppsala school’s “psychic distance” (e.g. cultural distance, lack of trust rather than on firm-specific advantages (Rugman, 1980; 2006. Key-words: international entry mode; emerging markets; subsidiary; joint-venture; India; Vietnam

  14. Internet portals as portfolios of entry options

    NARCIS (Netherlands)

    Perotti, E.C.; Rossetto, S.

    2000-01-01

    We investigate the valuation of platform investment, such as a software operating system or an Internet portal WebPage. Platform investment is the creation of an innovative distribution and production infrastructure, which increases access to customers; as a result it reduces entry costs in related

  15. Internet portals as portfolios of entry options

    NARCIS (Netherlands)

    Perotti, E.C.; Rossetto, S.

    2000-01-01

    We investigate the valuation of platform investment, such as a software operating system or an Internet portal WebPage. Platform investment is the creation of an innovative distribution and production infrastructure, which increases access to customers; as a result it reduces entry costs in related

  16. Flavivirus cell entry and membrane fusion

    NARCIS (Netherlands)

    Smit, Jolanda M.; Moesker, Bastiaan; Rodenhuis-Zybert, Izabela; Wilschut, Jan

    2011-01-01

    Flaviviruses, such as dengue virus and West Nile virus, are enveloped viruses that infect cells through receptor-mediated endocytosis and fusion from within acidic endosomes. The cell entry process of flaviviruses is mediated by the viral E glycoprotein. This short review will address recent advance

  17. Foreign Entry and Heterogeneous Growth of Firms

    DEFF Research Database (Denmark)

    Deng, Paul Duo; Jefferson, Gary H.

    distance from foreign firms. Domestic firms with smaller technological distance from their foreign counterparts tend to experience faster productivity growth, while firms with larger technological distance tend to lag further behind. We test this hypothesis using a unique firm-level data of Chinese...... manufacturing. Our empirical results confirm that foreign entry indeed generates strong heterogeneous growth patterns among domestic firms....

  18. Estimating the Effects of Delayed Entry into Higher Education: A Discussion

    DEFF Research Database (Denmark)

    Humlum, Maria Knoth

    2007-01-01

    In Denmark many high school graduates choose to delay their entry into higher education. A number of studies have investigated the effects of the delay on the educational and labour market careers. The existing studies are likely to suffer from selection bias, and this problem is unlikely...... to be remedied by use of control variables. More advanced techniques that can eliminate the selection bias are needed in order to identify causal effects. For policy purposes it would thus be preferable to focus on the economic losses associated with the years lost in the skilled labour market which...

  19. Happy Cycling

    DEFF Research Database (Denmark)

    Geert Jensen, Birgitte; Nielsen, Tom

    2013-01-01

    og Interaktions Design, Aarhus Universitet under opgave teamet: ”Happy Cycling City – Aarhus”. Udfordringen i studieopgaven var at vise nye attraktive løsningsmuligheder i forhold til cyklens og cyklismens integration i byrum samt at påpege relationen mellem design og overordnede diskussioner af...

  20. Glacial cycles

    DEFF Research Database (Denmark)

    Kaufmann, R. K.; Juselius, Katarina

    and compare the accuracy of their in-sample simulations. Results indicate that strong statistical associations between endogenous climate variables are not enough for statistical models to reproduce glacial cycles. Rather, changes in solar insolation associated with changes in Earth's orbit are needed...

  1. Koszul cycles

    CERN Document Server

    Bruns, Winfreid; Römer, Tim

    2010-01-01

    We prove regularity bounds for Koszul cycles holding for every ideal of dimension at most 1 in a polynomial ring. We generalize the lower bound for the Green-Lazarsfeld index of Veronese rings we proved in arXiv:0902.2431 to the multihomogeneous setting.

  2. Renal elimination of organic anions in cholestasis

    Institute of Scientific and Technical Information of China (English)

    Adriana Mónica Tortes

    2008-01-01

    The disposition of most drugs is highly dependent on specialized transporters.OAT1 and OAT3 are two organic anion transporters expressed in the basolateral membrane of renal proximal tubule cells,identified as contributors to xenobiotic and endogenous organic anion secretion.It is well known that cholestasis may cause renal damage.Impairment of kidney function produces modifications in the renal elimination of drugs.Recent studies have demonstrated that the renal abundance of OAT1 and OAT3 plays an important role in the renal elimination of organic anions in the presence of extrahepatic cholestasis.Time elapsed after obstructive cholestasis has an important impact on the regulation of both types of organic anion transporters.The renal expression of OAT1 and OAT3 should be taken into account in order to improve pharmacotherapeutic efficacy and to prevent drug toxicity during the onset of this hepatic disease.

  3. Eliminative behaviour of dairy cows at pasture

    DEFF Research Database (Denmark)

    Whistance, Lindsay Kay; Sinclair, Liam A.; Arney, David Richard

    2011-01-01

    within 30 s of an eliminative event. Intentional, incidental or no avoidance of faeces was also recorded for each event. Activity, characterised as static (lying, grazing or loafing), or active (moving to a different area of field, going to drink and catching up with herd) was also recorded. Of the 437...... going to drink or catching up with the herd. Overall, standing to defaecate and moving forward......Despite a strong avoidance of grazing near dung patches, cattle have traditionally been considered not to avoid bodily contact with faeces, regardless of any risk of disease. Little is understood of the behaviour of pasture-kept dairy cows at the time of defaecation and therefore, the eliminative...

  4. Twin-image elimination apparatus and method

    OpenAIRE

    1996-01-01

    The twin-image elimination apparatus of the present invention comprises (a) a scanning light source for emitting a scanning light beam; (b) an interference device which converts the scanning light beam from the scanning light source into a spherical wave and a plane wave having temporal frequencies different from each other and combines the spherical and plane waves together; (c) a scanner for scanning an object with the combined light beam from the interference device; (d) a photodetector fo...

  5. An Elimination of Resonance in Electric Drives

    Directory of Open Access Journals (Sweden)

    Michal Malek

    2011-01-01

    Full Text Available Flexible couplings together with resonance phenomenon are present mainly in the field of servodrives where high accuracy and dynamic requirements are crucial. When dynamics doesn’t correlate with mechanical system design, unwanted frequencies in the system are exited. Sometimes we haven’t conditions (whether material or space to design mechanical system with resonant frequencies too high to be exited. In that case we must choose compensating methods which can eliminate these phenomenons. This paper is dedicated to them.

  6. Hitting Hotspots: Spatial Targeting of Malaria for Control and Elimination

    NARCIS (Netherlands)

    Bousema, T.; Griffin, J.T.; Sauerwein, R.W.; Smith, D.L.; Churcher, T.S.; Takken, W.; Ghani, A.; Drakeley, C.; Gosling, R.

    2012-01-01

    Current malaria elimination guidelines are based on the concept that malaria transmission becomes heterogeneous in the later phases of malaria elimination [1]. In the pre-elimination and elimination phases, interventions have to be targeted to entire villages or towns with higher malaria incidence

  7. Hitting Hotspots: Spatial Targeting of Malaria for Control and Elimination

    NARCIS (Netherlands)

    Bousema, T.; Griffin, J.T.; Sauerwein, R.W.; Smith, D.L.; Churcher, T.S.; Takken, W.; Ghani, A.; Drakeley, C.; Gosling, R.

    2012-01-01

    Current malaria elimination guidelines are based on the concept that malaria transmission becomes heterogeneous in the later phases of malaria elimination [1]. In the pre-elimination and elimination phases, interventions have to be targeted to entire villages or towns with higher malaria incidence u

  8. Hitting Hotspots: Spatial Targeting of Malaria for Control and Elimination

    NARCIS (Netherlands)

    Bousema, T.; Griffin, J.T.; Sauerwein, R.W.; Smith, D.L.; Churcher, T.S.; Takken, W.; Ghani, A.; Drakeley, C.; Gosling, R.

    2012-01-01

    Current malaria elimination guidelines are based on the concept that malaria transmission becomes heterogeneous in the later phases of malaria elimination [1]. In the pre-elimination and elimination phases, interventions have to be targeted to entire villages or towns with higher malaria incidence u

  9. Potential economic benefits of eliminating canine rabies.

    Science.gov (United States)

    Shwiff, Stephanie; Hampson, Katie; Anderson, Aaron

    2013-05-01

    Although canine rabies has been eliminated from industrialized countries, infected dogs remain the primary source of human and livestock exposures in Asia, Africa and much of South America. Human deaths are the most important direct economic impact of canine rabies, followed by livestock losses and the cost of PEP, while expenses associated with dog vaccination and control are major indirect impacts. The global burden of rabies disproportionately affects Asia, which experiences more than half of human rabies deaths and approximately 65% of livestock losses, and performs more than 90% of postexposure prophylaxis (PEP). Africa is second to Asia in terms of human deaths and livestock losses, but administers the least number of PEPs of the three regions. Recent experience in Latin America shows that efforts to reduce human deaths from rabies through expanded dog vaccination and improved access to PEP result in significant monetary savings. The elimination of canine rabies would lead to major economic benefits in developing countries that are often the least capable of dealing with the disease. This article forms part of a symposium in Antiviral Research on the elimination of canine rabies. Published by Elsevier B.V.

  10. Role of genomics in eliminating health disparities

    Directory of Open Access Journals (Sweden)

    Meghana V Kashyap

    2015-01-01

    Full Text Available The Texas Center for Health Disparities, a National Institute on Minority Health and Health Disparities Center of Excellence, presents an annual conference to discuss prevention, awareness education, and ongoing research about health disparities both in Texas and among the national population. The 2014 Annual Texas Conference on Health Disparities brought together experts in research, patient care, and community outreach on the "Role of Genomics in Eliminating Health Disparities." Rapid advances in genomics and pharmacogenomics are leading the field of medicine to use genetics and genetic risk to build personalized or individualized medicine strategies. We are at a critical juncture of ensuring such rapid advances benefit diverse populations. Relatively few forums have been organized around the theme of the role of genomics in eliminating health disparities. The conference consisted of three sessions addressing "Gene-Environment Interactions and Health Disparities," "Personalized Medicine and Elimination of Health Disparities," and "Ethics and Public Policy in the Genomic Era." This article summarizes the basic science, clinical correlates, and public health data presented by the speakers.

  11. Planning of elimination of emergency consequences

    Directory of Open Access Journals (Sweden)

    S. Kovalenko

    2015-05-01

    Full Text Available Introduction. The volume of useful information in the planning of elimination of emergency consequences process is reasonable to assess with calculatory problems and mathematical models. Materials and methods. The expert survey method is used to calculate quantitative values of probability and to determine the optimal solution before the information in condition is received. Results. It is determined that the quality of the solution of elimination emergency consequences depends primarily on the number of factors that are taken into account in particular circumstances of the situation; on the level of information readiness of control bodies to take decision to eliminate emergency consequences as soon as possible and to consider several options for achieving reasonableness and concreteness of a particular decision. The ratio between volume of useful information collected and processed during operation planning which is required for identifying optimal solution is calculated. This ratio allows to construct a graph of probability of identifying a solution in existing environment and probability value of identifying optimal solution before information in P*condition is obtained. This graph also shows the ratio volume of useful information collected and processed during operation planning and necessary volume of information for identifying optimal solution. Conclusion. The results of this research can be used for improving control bodies decisions to ensure safe working conditions for employees of food industry.

  12. Graph-based local elimination algorithms in discrete optimization

    CERN Document Server

    Shcherbina, Oleg

    2009-01-01

    The aim of this paper is to provide a review of structural decomposition methods in discrete optimization and to give a unified framework in the form of local elimination algorithms (LEA). This paper is organized as follows. Local elimination algorithms for discrete optimization (DO) problems (DOPs) with constraints are considered; a classification of dynamic programming computational procedures is given. We introduce Elimination Game and Elimination tree. Application of bucket elimination algorithm from constraint satisfaction (CS) to solving DOPs is done. We consider different local elimination schemes and related notions. Clustering that merges several variables into single meta-variable defines a promising approach to solve DOPs. This allows to create a quotient (condensed) graph and apply a local block elimination algorithm. In order to describe a block elimination process, we introduce Block Elimination Game. We discuss the connection of aforementioned local elimination algorithmic schemes and a way of ...

  13. Analysis of Effectiveness of Phoenix Entry Reaction Control System

    Science.gov (United States)

    Dyakonov, Artem A.; Glass, Christopher E.; Desai, Prasun, N.; VanNorman, John W.

    2008-01-01

    Interaction between the external flowfield and the reaction control system (RCS) thruster plumes of the Phoenix capsule during entry has been investigated. The analysis covered rarefied, transitional, hypersonic and supersonic flight regimes. Performance of pitch, yaw and roll control authority channels was evaluated, with specific emphasis on the yaw channel due to its low nominal yaw control authority. Because Phoenix had already been constructed and its RCS could not be modified before flight, an assessment of RCS efficacy along the trajectory was needed to determine possible issues and to make necessary software changes. Effectiveness of the system at various regimes was evaluated using a hybrid DSMC-CFD technique, based on DSMC Analysis Code (DAC) code and General Aerodynamic Simulation Program (GASP), the LAURA (Langley Aerothermal Upwind Relaxation Algorithm) code, and the FUN3D (Fully Unstructured 3D) code. Results of the analysis at hypersonic and supersonic conditions suggest a significant aero-RCS interference which reduced the efficacy of the thrusters and could likely produce control reversal. Very little aero-RCS interference was predicted in rarefied and transitional regimes. A recommendation was made to the project to widen controller system deadbands to minimize (if not eliminate) the use of RCS thrusters through hypersonic and supersonic flight regimes, where their performance would be uncertain.

  14. Rho GTPases regulate PRK2/PKN2 to control entry into mitosis and exit from cytokinesis.

    Science.gov (United States)

    Schmidt, Anja; Durgan, Joanne; Magalhaes, Ana; Hall, Alan

    2007-03-21

    Rho GTPases regulate multiple signal transduction pathways that influence many aspects of cell behaviour, including migration, morphology, polarity and cell cycle. Through their ability to control the assembly and organization of the actin and microtubule cytoskeletons, Rho and Cdc42 make several key contributions during the mitotic phase of the cell cycle, including spindle assembly, spindle positioning, cleavage furrow contraction and abscission. We now report that PRK2/PKN2, a Ser/Thr kinase and Rho/Rac effector protein, is an essential regulator of both entry into mitosis and exit from cytokinesis in HeLa S3 cells. PRK2 is required for abscission of the midbody at the end of the cell division cycle and for phosphorylation and activation of Cdc25B, the phosphatase required for activation of mitotic cyclin/Cdk1 complexes at the G2/M transition. This reveals an additional step in the mammalian cell cycle controlled by Rho GTPases.

  15. Learning, diffusion and the industry life cycle

    OpenAIRE

    Zhu Wang

    2006-01-01

    An industry typically experiences initial mass entry and later shakeout of producers over its life cycle. It can be explained as a competitive equilibrium outcome driven by the dynamic interaction between technology progress and demand diffusion. When a new product is introduced, high-income consumers tend to adopt it first. Technology then improves with cumulative output and demand growth generates S-shaped diffusion as the product penetrates lower-income groups. Eventually fewer new adopter...

  16. Analytic Guidance for the First Entry in a Skip Atmospheric Entry

    Science.gov (United States)

    Garcia-Llama, Eduardo

    2007-01-01

    This paper presents an analytic method to generate a reference drag trajectory for the first entry portion of a skip atmospheric entry. The drag reference, expressed as a polynomial function of the velocity, will meet the conditions necessary to fit the requirements of the complete entry phase. The generic method proposed to generate the drag reference profile is further simplified by thinking of the drag and the velocity as density and cumulative distribution functions respectively. With this notion it will be shown that the reference drag profile can be obtained by solving a linear algebraic system of equations. The resulting drag profile is flown using the feedback linearization method of differential geometric control as guidance law with the error dynamics of a second order homogeneous equation in the form of a damped oscillator. This approach was first proposed as a revisited version of the Space Shuttle Orbiter entry guidance. However, this paper will show that it can be used to fly the first entry in a skip entry trajectory. In doing so, the gains in the error dynamics will be changed at a certain point along the trajectory to improve the tracking performance.

  17. Entry Location and Entry Timing (ELET Decision Model for International Construction Firms

    Directory of Open Access Journals (Sweden)

    Che Maznah Mat Isa

    2014-09-01

    Full Text Available This paper proposes a model for entry location (EL and entry timing (ET decisions to guide construction firms in accessing targeted international markets.  Neglecting to properly choose the right combination of the entry location and entry timing (ELET decisions can lead to poor performance of the firms’ international ventures.  The sampling frame was from the Malaysian construction firms that have undertaken and completed projects abroad.  Survey questionnaires sent to 115 firms registered with Construction Industry Development Board (CIDB Malaysia, operating in more than 50 countries, achieved a 39.1 per cent response rate. Based on a comprehensive statistical analysis of survey data it was found that the mutually inclusive significant factors that influenced the firms’ ELET decisions were: the firm’s ability to assess market signals and opportunities, international experience, financial capacity, competencies and capabilities (project management, specialist expertise and technology, resources (level of knowledge based on research and development, experience in similar works, financial support from the home country banks, technical complexities of projects and availability of funds for projects.  Hence, the present research builds on and extends the literature on the ELET decisions in a more integrated way. Keywords: Entry location, entry timing, resource-based view, international markets, Malaysian construction firms.

  18. The cell cycle rallies the transcription cycle: Cdc28/Cdk1 is a cell cycle-regulated transcriptional CDK.

    Science.gov (United States)

    Chymkowitch, Pierre; Enserink, Jorrit M

    2013-01-01

    In the budding yeast Saccharomyces cerevisiae, the cyclin-dependent kinases (CDKs) Kin28, Bur1 and Ctk1 regulate basal transcription by phosphorylating the carboxyl-terminal domain (CTD) of RNA polymerase II. However, very little is known about the involvement of the cell cycle CDK Cdc28 in the transcription process. We have recently shown that, upon cell cycle entry, Cdc28 kinase activity boosts transcription of a subset of genes by directly stimulating the basal transcription machinery. Here, we discuss the biological significance of this finding and give our view of the kinase-dependent role of Cdc28 in regulation of RNA polymerase II.

  19. Adaptive Deployable Entry and Placement Technology (ADEPT) Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The ADEPT Project is developing a mechanically deployable low-ballistic coefficient aeroshell entry system to perform entry descent landing (EDL) functions for...

  20. Design and Simulation Tools for Planetary Atmospheric Entry Vehicles Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Atmospheric entry is one of the most critical phases of flight during planetary exploration missions. During the design of an entry vehicle, experimental and...

  1. Sulfur cycle

    Digital Repository Service at National Institute of Oceanography (India)

    LokaBharathi, P.A.

    :1, the reductive assimilation of sulfate is less important than nitrate. Assimilatory reduction is common among organisms and does not lead to the production of sulfide. The eight-electron reduction of sulfate to sulfide pro- ceeds in different stages. As the ion...; Biogeochemical Approaches to Environmental Risk Assessment; Biogeochemical Models; Biomagnification; Carbon Cycle; Classification and Regression Trees; Climate Change 1: Short-Term Dynamics; Constructed Wetlands, Subsurface Flow; Constructed Wetlands, Surface...

  2. Dependency of radon entry on pressure difference

    Science.gov (United States)

    Kokotti, H.; Kalliokoski, P.; Jantunen, M.

    Radon levels, ventilation rate and pressure differences were monitored continuously in four apartment houses with different ventilation systems. Two of them were ventilated by mechanical exhaust, one by mechanical supply and exhaust, and one by natural ventilation. The two-storey houses were constructed from concrete elements on a slab and located on a gravel esker. It was surprising to find that increasing the ventilation rate increased levels of radon in the apartments. Increased ventilation caused increased outdoor-indoor pressure difference, which in turn increased the entry rate of radon and counteracted the diluting effect of ventilation. The increase was significant when the outdoor-indoor pressure difference exceeded 5 Pa. Especially in the houses with mechanical exhaust ventilation the pressure difference was the most important factor of radon entry rate, and contributed up to several hundred Bq m -3 h -1.

  3. Prototipicality in entries of school dictionaries

    Directory of Open Access Journals (Sweden)

    Everton Castro de Almeida

    2015-01-01

    Full Text Available Theoretical Lexicography is the discipline which, in a general way, studies and criticizes the dictionaries. It is open to the theoretical contribution from other fields of research, such as Text Linguistics, Pragmatics, and Multimodality, once they may show another lexical dimension. Thus, we base our analysis on the theoretical support from Prototypes Theory, from cognitive sciences. In that sense, this paper aims to identify and describe the prototypicality in entries from the five type 3 dictionaries from the PNLD 2012 (National Plan for the Didactic Books. In order to do so, we use theories from Pontes (2008; 2009 and Geeraerts (2007. We analyze five entries, one from each dictionary type 3.

  4. 14 CFR 375.24 - Entry and clearance.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Entry and clearance. 375.24 Section 375.24 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) SPECIAL... Entry and clearance. All U.S. entry and clearance requirements for aircraft, passengers, crews,...

  5. 19 CFR 143.35 - Procedure for electronic entry summary.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Procedure for electronic entry summary. 143.35 Section 143.35 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) SPECIAL ENTRY PROCEDURES Electronic Entry Filing § 143.35...

  6. 77 FR 17492 - Expansion of Global Entry to Additional Airports

    Science.gov (United States)

    2012-03-26

    ... selection process, and the initial airport locations. See 77 FR 5681 and 8 CFR 235.12. Travelers who wish to... SECURITY U.S. Customs and Border Protection Expansion of Global Entry to Additional Airports AGENCY: U.S... as Global Entry, at twenty major U.S. airports. Global Entry allows pre-approved,...

  7. 40 CFR 90.1207 - Entry and access.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Entry and access. 90.1207 Section 90... § 90.1207 Entry and access. (a) To allow the Administrator to determine whether a manufacturer is... maintenance. (c) The provisions of § 90.705(c), (d), (e), (f) and (g) also apply to entry and access...

  8. Maximum Atmospheric Entry Angle for Specified Retrofire Impulse

    Directory of Open Access Journals (Sweden)

    T. N. Srivastava

    1969-07-01

    Full Text Available Maximum atmospheric entry angles for vehicles initially moving in elliptic orbits are investigated and it is shown that tangential retrofire impulse at the apogee results in the maximum entry angle. Equivalence of maximizing the entry angle and minimizing the retrofire impulse is also established.

  9. 19 CFR 143.16 - Substitution of warehouse entry.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Substitution of warehouse entry. 143.16 Section 143.16 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT... warehouse entry. The importer may substitute an entry for warehouse at any time within 1 year from the...

  10. 50 CFR 91.16 - Submission procedures for entry.

    Science.gov (United States)

    2010-10-01

    ... (CONTINUED) MISCELLANEOUS PROVISIONS MIGRATORY BIRD HUNTING AND CONSERVATION STAMP CONTEST Procedures for.... Each entry must be accompanied by a non-refundable entrance fee and a completed and signed Reproduction... the Reproduction Rights Agreement must be attached to the back of the entry. (b) Each entry should...

  11. 31 CFR 337.6 - Conversions to book-entry.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Conversions to book-entry. 337.6... HOUSING ADMINISTRATION DEBENTURES Certificated Debentures § 337.6 Conversions to book-entry. Upon implementation of the book-entry debenture system, to be announced in advance by separate public notice, all...

  12. Sport entries & qualification manual: Innsbruck 2012 Youth Olympic Games

    OpenAIRE

    2015-01-01

    Sport entries constitute an important part of the delegation registration process of the Innsbruck 2012 Winter Youth Olympic Games. This manual aims to introduce to NOCs the process, relevant policies and requirements regarding sport entries so as to ensure that NOCs can successfully complete the entries for their athletes to the Innsbruck 2012 Winter Youth Olympic Games.

  13. The global cost of eliminating avoidable blindness

    Directory of Open Access Journals (Sweden)

    Kirsten L Armstrong

    2012-01-01

    Full Text Available Aims : To complete an initial estimate of the global cost of eliminating avoidable blindness, including the investment required to build ongoing primary and secondary health care systems, as well as to eliminate the ′backlog′ of avoidable blindness. This analysis also seeks to understand and articulate where key data limitations lie. Materials and Methods : Data were collected in line with a global estimation approach, including separate costing frameworks for the primary and secondary care sectors, and the treatment of backlog. Results : The global direct health cost to eliminate avoidable blindness over a 10-year period from 2011 to 2020 is estimated at $632 billion per year (2009 US$. As countries already spend $592 billion per annum on eye health, this represents additional investment of $397.8 billion over 10 years, which is $40 billion per year or $5.80 per person for each year between 2010 and 2020. This is concentrated in high-income nations, which require 68% of the investment but comprise 16% of the world′s inhabitants. For all other regions, the additional investment required is $127 billion. Conclusions : This costing estimate has identified that low- and middle-income countries require less than half the additional investment compared with high-income nations. Low- and middle-income countries comprise the greater investment proportion in secondary care whereas high-income countries require the majority of investment into the primary sector. However, there is a need to improve sector data. Investment in better data will have positive flow-on effects for the eye health sector.

  14. Reducing allergic symptoms through eliminating subgingival plaque

    Directory of Open Access Journals (Sweden)

    Haryono Utomo

    2008-12-01

    Full Text Available Background: Elimination of subgingival plaque for prevention and treatment of periodontal diseases through scaling is a routine procedure. It is also well-known that periodontal disease is related to systemic diseases. Nevertheless, the idea how scaling procedures also able to reduce allergic symptoms i.e. eczema and asthma, is not easily accepted, because it is contradictory to the “hygiene hypothesis”. However, since allergic symptoms also depend on variable factors such as genetic, environmental and infection factors; every possible effort to eliminate or avoid from these factors had to be considered. Subgingival plaque is a source of infection, especially the Gram-negative bacteria that produced endotoxin (lipopolysaccharides, LPS, a potential stimulator of immunocompetent cells, which may also related to allergy, such as mast cells and basophils. In addition, it also triggers the “neurogenic switching” mechanism which may be initiated from chronic gingivitis. Objective: This case report may explain the possible connection between subgingival plaque and allergy based on evidence-based cases. Case: Two adult siblings who suffered from chronic gingivitis also showed different manifestations of allergy that were allergic dermatitis and asthma for years. They were also undergone unsuccessful medical treatment for years. Oral and topical corticosteroids were taken for dermatitis and inhalation for asthma. Case Management: Patients were conducted deep scaling procedures, allergic symptoms gradually diminished in days even though without usual medications. Conclusion: Concerning to the effectiveness of scaling procedures which concomitantly eliminate subgingival plaque in allergic patients, it concluded that this concept is logical. Nevertheless, further verification and collaborated study with allergic expert should be done.

  15. Hypersonic Flight Mechanics. [for atmospheric entry trajectories

    Science.gov (United States)

    Busemann, A.; Vinh, N. X.; Culp, R. D.

    1976-01-01

    The effects of aerodynamic forces on trajectories at orbital speeds are discussed in terms of atmospheric models. The assumptions for the model are spherical symmetry, nonrotating, and an exponential atmosphere. The equations of flight, and the performance in extra-atmospheric flight are discussed along with the return to the atmosphere, and the entry. Solutions of the exact equations using directly matched asymptotic expansions are presented.

  16. Unified treatment of lifting atmospheric entry

    Science.gov (United States)

    Nachtsheim, P. R.; Lehman, L. L.

    1980-01-01

    This paper presents a unified treatment of the effect of lift on peak acceleration during atmospheric entry. Earlier studies were restricted to different regimes because of approximations invoked to solve the same transcendental equation. This paper shows the connection between the earlier studies by employing a general expression for the peak acceleration and obtains solutions to the transcendental equation without invoking the earlier approximations. Results are presented and compared with earlier studies where appropriate.

  17. Elimination of Sexual Discrimination in English Study

    Institute of Scientific and Technical Information of China (English)

    蒋小文

    2008-01-01

    Gender discrimination is a social attitude that is re- flected in language. The representation of gender discrimination in the English language has a variety of forms, and can be eliminated by vari- ous means. English teachers are supposed to observe these phenomenon, avoiding using sexual prejudical words and helping students to learn to use neuter words so that they can communicate with English native speakers effortlessly without hurting the latter. This thesis is tending to talk about the ways of removing sexism in English study.

  18. Redundancy Elimination in DTN via ACK Mechanism

    Directory of Open Access Journals (Sweden)

    Xiqing Zhang

    2015-08-01

    Full Text Available The traditional routing protocols for delay tolerant networks (DTN usually take the strategy of spreading multiple copies of one message to the networks. When one copy reaches destination, the transmission of other copies not only waste the bandwidth but also deprive other messages of the opportunities for transmission. This paper brings up a mechanism to eliminate the redundant copies. By adding an acknowledge field to the packet header to delete redundant copies, it can degrade the network overhead while improve the delivery ratio. Simulation results confirm that the proposed method can improve the performance of epidemic and Spray and Wait routing protocol.

  19. Waveguide arrangements based on adiabatic elimination

    Energy Technology Data Exchange (ETDEWEB)

    Suchowski, Haim; Mrejen, Michael; Wu, Chihhui; Zhang, Xiang

    2016-09-13

    This disclosure provides systems, methods, and apparatus related to nanophotonics. In one aspect, an arrangement of waveguides includes a substrate and three waveguides. Each of the three waveguides may be a linear waveguide. A second waveguide is positioned between a first waveguide and a third waveguide. The dimensions and positions of the first, the second, and the third waveguides are specified to substantially eliminate coupling between the first waveguide and the third waveguide over a distance of about 1 millimeter to 2 millimeters along lengths of the first waveguide, the second waveguide, and the third waveguide.

  20. Nuclear Chk1 prevents premature mitotic entry.

    Science.gov (United States)

    Matsuyama, Makoto; Goto, Hidemasa; Kasahara, Kousuke; Kawakami, Yoshitaka; Nakanishi, Makoto; Kiyono, Tohru; Goshima, Naoki; Inagaki, Masaki

    2011-07-01

    Chk1 inhibits the premature activation of the cyclin-B1-Cdk1. However, it remains controversial whether Chk1 inhibits Cdk1 in the centrosome or in the nucleus before the G2-M transition. In this study, we examined the specificity of the mouse monoclonal anti-Chk1 antibody DCS-310, with which the centrosome was stained. Conditional Chk1 knockout in mouse embryonic fibroblasts reduced nuclear but not centrosomal staining with DCS-310. In Chk1(+/myc) human colon adenocarcinoma (DLD-1) cells, Chk1 was detected in the nucleus but not in the centrosome using an anti-Myc antibody. Through the combination of protein array and RNAi technologies, we identified Ccdc-151 as a protein that crossreacted with DCS-310 on the centrosome. Mitotic entry was delayed by expression of the Chk1 mutant that localized in the nucleus, although forced immobilization of Chk1 to the centrosome had little impact on the timing of mitotic entry. These results suggest that nuclear but not centrosomal Chk1 contributes to correct timing of mitotic entry.

  1. Cell entry by human pathogenic arenaviruses.

    Science.gov (United States)

    Rojek, Jillian M; Kunz, Stefan

    2008-04-01

    The arenaviruses Lassa virus (LASV) in Africa and Machupo (MACV), Guanarito (GTOV) and Junin viruses (JUNV) in South America cause severe haemorrhagic fevers in humans with fatality rates of 15-35%. The present review focuses on the first steps of infection with human pathogenic arenaviruses, the interaction with their cellular receptor molecules and subsequent entry into the host cell. While similarities exist in genomic organization, structure and clinical disease caused by pathogenic Old World and New World arenaviruses these pathogens use different primary receptors. The Old World arenaviruses employ alpha-dystroglycan, a cellular receptor for proteins of the extracellular matrix, and the human pathogenic New World arenaviruses use the cellular cargo receptor transferrin receptor 1. While the New World arenavirus JUNV enters cells via clathrin-dependent endocytosis, evidence occurred for clathrin-independent entry of the prototypic Old World arenavirus lymphocytic choriomeningitis virus. Upon internalization, arenaviruses are delivered to the endosome, where pH-dependent membrane fusion is mediated by the envelope glycoprotein (GP). While arenavirus GPs share characteristics with class I fusion GPs of other enveloped viruses, unusual mechanistic features of GP-mediated membrane fusion have recently been discovered for arenaviruses with important implications for viral entry.

  2. Kinetics of virus entry by endocytosis

    Science.gov (United States)

    Zhdanov, Vladimir P.

    2015-04-01

    Entry of virions into the host cells is either endocytotic or fusogenic. In both cases, it occurs via reversible formation of numerous relatively weak bonds resulting in wrapping of a virion by the host membrane with subsequent membrane rupture or scission. The corresponding kinetic models are customarily focused on the formation of bonds and do not pay attention to the energetics of the whole process, which is crucially dependent, especially in the case of endocytosis, on deformation of actin filaments forming the cytoskeleton of the host cell. The kinetic model of endocytosis, proposed by the author, takes this factor into account and shows that the whole process can be divided into a rapid initial transient stage and a long steady-state stage. The entry occurs during the latter stage and can be described as a first-order reaction. Depending on the details of the dependence of the grand canonical potential on the number of bonds, the entry can be limited either by the interplay of bond formation and membrane rupture (or scission) or by reaching a maximum of this potential.

  3. Filovirus tropism: Cellular molecules for viral entry

    Directory of Open Access Journals (Sweden)

    Ayato eTakada

    2012-02-01

    Full Text Available In human and nonhuman primates, filoviruses (Ebola and Marburg viruses cause severe hemorrhagic fever.Recently, other animals such as pigs and some species of fruit bats have also been shown to be susceptible to these viruses. While having a preference for some cell types such as hepatocytes, endothelial cells, dendritic cells, monocytes, and macrophages, filoviruses are known to be pantropic in infection of primates. The envelope glycoprotein (GP is responsible for both receptor binding and fusion of the virus envelope with the host cell membrane. It has been demonstrated that filovirus GP interacts with multiple molecules for entry into host cells, whereas none of the cellular molecules so far identified as a receptor/coreceptor fully explains filovirus tissue tropism and host range. Available data suggest that the mucin-like region (MLR on GP plays an important role in attachment to the preferred target cells, whose infection is likely involved in filovirus pathogenesis, whereas the MLR is not essential for the fundamental function of the GP in viral entry into cells in vitro. Further studies elucidating the mechanisms of cellular entry of filoviruses may shed light on the development of strategies for prophylaxis and treatment of Ebola and Marburg hemorrhagic fevers.

  4. Entry of large nanoparticles into cells aided by nanoscale mechanical stimulation

    Science.gov (United States)

    Vaidyanathan, Ramanathan; Curtis, Adam; Mullin, Margaret

    2011-10-01

    Nanoparticle entry into the cell depends on the surface charge and also on their size. Here, we report the entry of large magnetic nanoparticles (500 nm mean diameter) into the cell, being mediated by a mechanical stimulus supplied to the culture flasks. Investigations were carried out at 2-10 Hz frequency range with the vertical excursions ranging from 5 to 20 nm. Mechanical stimulation was found to aid the entry of both positive and negatively charged nanoparticles over a frequency range of 2-10 Hz. Transmission electron microscopy analysis indicated that, the stimulated samples could possibly mediate particle uptake through membrane invaginations, while the control samples indicated particles at the cell periphery, just outside the cell membrane. Mechanical stimulation had no significant effect on the cell morphology. Bromodeoxyuridine incorporation resulted in an increase in the proportion of S-phase in the stimulated samples compared with the controls, suggesting a reduction in the cell cycle duration. Mechanical stimulation could very well extend its effects to nanoscale cellular movements, and also facilitate the entry of large magnetic nanoparticle. This could be an interesting prospect for nanoparticle mediated drug delivery.

  5. High-throughput screening of viral entry inhibitors using pseudotyped virus.

    Science.gov (United States)

    Basu, Arnab; Mills, Debra M; Bowlin, Terry L

    2010-12-01

    Virus entry into a host cell is an attractive target for therapy because propagation of virus can be blocked at an early stage, minimizing chances for the virus to acquire drug resistance. Anti-infective drug discovery for BSL-4 viruses like Ebola or Lassa hemorrhagic fever virus presents challenges due to the requirement for a BSL-4 laboratory containment facility. Pseudotyped viruses provide a surrogate model in which the native envelope glycoprotein of a BSL-2 level virus (e.g., vesicular stomatitis virus) is replaced with envelope glycoprotein of a foreign BSL-4 virus (e.g., Ebola virus). Because the envelope glycoprotein determines interaction of virus with its cellular receptors, pseudotyped viruses can mimic the viral entry process of the original virus. Moreover, they are competent for only a single cycle of infection, and therefore can be used in BSL-2 facilities. Pseudotyped viruses have been used in high-throughput screening of entry inhibitors for a number of BSL-4 level viruses. This unit includes protocols for preparing pseudotyped viruses using lentiviral vectors and use of pseudotyped viruses for high-throughput screening of viral entry inhibitors.

  6. Apparatus and Method for Elimination of Polarization-Induced Fading in Fiber-optic Sensor System

    Science.gov (United States)

    Chan, Hon Man (Inventor); Parker, Jr., Allen R. (Inventor)

    2015-01-01

    The invention is an apparatus and method of eliminating polarization-induced fading in interferometric fiber-optic sensor system having a wavelength-swept laser optical signal. The interferometric return signal from the sensor arms are combined and provided to a multi-optical path detector assembly and ultimately to a data acquisition and processing unit by way of a switch that is time synchronized with the laser scan sweep cycle.

  7. 19 CFR 149.6 - Entry and entry summary documentation and Importer Security Filing submitted via a single...

    Science.gov (United States)

    2010-04-01

    ... Security Filing submitted via a single electronic transmission. 149.6 Section 149.6 Customs Duties U.S... submitted via a single electronic transmission. If the Importer Security Filing is filed pursuant to § 149.2 of this part via the same electronic transmission as entry or entry/entry summary...

  8. Gas-liquid vortex separator-eliminator

    Energy Technology Data Exchange (ETDEWEB)

    Hull, R.; Lane, J.A.

    1984-10-09

    Closed liquid circulating systems, which have supply and return lines and a circulating pump, have a problem when gas present in the circulating liquid. A device for removing the gas by insertion in the system includes an upright main body which has an upper chamber and a lower chamber. The separating horizontal wall has a gas discharge port in the center thereof. The horizontal inlet has an integral diffuser positioned immediately inside of the lower chamber. The integral diffuser is affixed at horizontal and vertical angles to the horizontal axis of the inlet. The horizontal outlet has a vertical pipe leg positioned inside of the lower chamber so that the lower segment thereof is positioned in the lower portion of the lower chamber and that the vertical axis of the lower segment of the vertical pipe leg is aligned in the vertical axis of the upright main body. A gas vent-eliminator is positioned within the upper chamber. The liquid enters the inlet and is forced tangentially against the side of and downwardly in the lower chamber so as to form a water vortex in the lower chamber whereby the gas in the liquid is released in the form of bubbles. The lower end of the vertical pipe leg is below where the bubbles form. The gas bubbles rise into the upper chamber and are purged into the atmosphere by means of the gas vent-eliminator.

  9. Eliminating ultrasonic interference from respiratory muscle EMG.

    Science.gov (United States)

    Platt, R S; Kieser, T M; Easton, P A

    1998-05-01

    Fine wire recordings of the respiratory muscle electromyogram are often employed to represent muscle activity, and recently ultrasound-sonomicrometry has become a common method of measuring length of respiratory muscles in both acute and chronic preparations. Although recording both EMG and sonomicrometry simultaneously has become standard practice, there has not been any consideration of the potential confounding influence of ultrasound noise upon the recorded EMG spectrum. Activation of the sonomicrometry-ultrasound tranducer introduces a high frequency, high amplitude voltage pulse plus harmonics, which can contaminate the EMG spectrum directly, as well as through aliasing when EMG is sampled directly digitally. We describe the use of a new, combined, wing stabilized sonomicrometry- and EMG measurement transducer to characterize exactly the influence of ultrasound upon the crural diaphragm EMG spectrum, and the development of digital filtering techniques which effectively eliminate the ultrasound interference. Two alternative methods of avoiding ultrasound-EMG interference are also considered. The isolation and elimination of ultrasound-sonomicrometry signal interference may be important in studies where EMG and length are measured together.

  10. Bell inequalities from variable elimination methods

    CERN Document Server

    Budroni, Costantino

    2011-01-01

    Tight Bell inequalities are facets of Pitowsky's correlation polytope and are usually obtained from its extreme points by solving the hull problem. Recently, Avis, Imai, Ito and Sasaki have proposed an alternative method, based on variable elimination, which overcomes some of the computational difficulties of the hull problem. However, this method can only be applied to the bipartite case. Here we present an algebraic derivation of the half-space representation for a family of convex polytopes, from which every correlation polytope can be obtained as a projection. As a result, variable elimination methods, e.g. the Fourier-Motzkin method, can be applied to obtain tight Bell inequalities in an n-party scenario. Similar ideas are shown to be applicable to a different kind of algebraic conditions. In particular, this analysis provides an explanation for the fact that only a finite number of families of Bell inequalities arise in scenarios where one experimenter can choose between an arbitrary number of measureme...

  11. Entry and Exit Dynamics of Nascent Business Owners

    DEFF Research Database (Denmark)

    Rocha, Vera; Carneiro, Anabela; Varum, Celeste

    2015-01-01

    in the labor market and entry modes shape the post-entry dynamics of nascent business owners. By differentiating between different entry and exit routes, this paper provides new evidence on different human capital patterns among nascent business owners and on key determinants of entrepreneurial survival. Our...... results suggest that different exit modes can be predicted by business owners’ entry route. Furthermore, different exit modes exhibit different duration dependence patterns according to the entry mode. Additionally, the paper shows that businesses started after a displacement episode are not necessarily...

  12. Exporting Complex Digital Products: Motives and Entry Modes

    DEFF Research Database (Denmark)

    Rask, Morten

    2005-01-01

    for digital product providers. However, other types of entry modes like what we call direct digital export with F2F-sales, direct digital export with F2F-support, and virtual sales subsidiary are entry modes that respond to a higher degree of pre- and postsales complexity.......When the product is digital, it will most often be distributed directly to the customer through the Internet, and therefore, the entry modes, considered in this paper, are different flavors of the entry mode called direct export: virtual export channel is generally understood as the entry mode...

  13. Exporting Complex Digital Products: Motives and Entry Modes

    DEFF Research Database (Denmark)

    Rask, Morten

    for digital product providers. However other types of entry modes like what wee call direct digital export with F2F-sales, direct digital export with F2F-support and virtual sales subsidiary are entry modes that respond to a higher degree of pre- and after-sales complexity.......When the product is digital, it will most often be distributed directly to the customer through the Internet, and therefore the entry modes, considered in this paper, are different flavors of the entry mode called direct export: Virtual export channel are generally understood as the entry mode...

  14. Exporting Complex Digital Products: Motives and Entry Modes

    DEFF Research Database (Denmark)

    Rask, Morten

    When the product is digital, it will most often be distributed directly to the customer through the Internet, and therefore the entry modes, considered in this paper, are different flavors of the entry mode called direct export: Virtual export channel are generally understood as the entry mode...... for digital product providers. However other types of entry modes like what wee call direct digital export with F2F-sales, direct digital export with F2F-support and virtual sales subsidiary are entry modes that respond to a higher degree of pre- and after-sales complexity....

  15. Exporting Complex Digital Products: Motives and Entry Modes

    DEFF Research Database (Denmark)

    Rask, Morten

    2005-01-01

    When the product is digital, it will most often be distributed directly to the customer through the Internet, and therefore, the entry modes, considered in this paper, are different flavors of the entry mode called direct export: virtual export channel is generally understood as the entry mode...... for digital product providers. However, other types of entry modes like what we call direct digital export with F2F-sales, direct digital export with F2F-support, and virtual sales subsidiary are entry modes that respond to a higher degree of pre- and postsales complexity....

  16. Lifting Entry & Atmospheric Flight (LEAF) System Concept Applications at Solar System Bodies With an Atmosphere

    Science.gov (United States)

    Lee, Greg; Polidan, Ronald; Ross, Floyd; Sokol, Daniel; Warwick, Steve

    2015-11-01

    Northrop Grumman and L’Garde have continued the development of a hypersonic entry, semi-buoyant, maneuverable platform capable of performing long-duration (months to a year) in situ and remote measurements at any solar system body that possesses an atmosphere.The Lifting Entry & Atmospheric Flight (LEAF) family of vehicles achieves this capability by using a semi-buoyant, ultra-low ballistic coefficient vehicle whose lifting entry allows it to enter the atmosphere without an aeroshell. The mass savings realized by eliminating the heavy aeroshell allows significantly more payload to be accommodated by the platform for additional science collection and return.In this presentation, we discuss the application of the LEAF system at various solar system bodies: Venus, Titan, Mars, and Earth. We present the key differences in platform design as well as operational differences required by the various target environments. The Venus implementation includes propulsive capability to reach higher altitudes during the day and achieves full buoyancy in the mid-cloud layer of Venus’ atmosphere at night.Titan also offers an attractive operating environment, allowing LEAF designs that can target low or medium altitude operations, also with propulsive capabilities to roam within each altitude regime. The Mars version is a glider that descends gradually, allowing targeted delivery of payloads to the surface or high resolution surface imaging. Finally, an Earth version could remain in orbit in a stowed state until activated, allowing rapid response type deployments to any region of the globe.

  17. MEK1 inactivates Myt1 to regulate Golgi membrane fragmentation and mitotic entry in mammalian cells.

    Science.gov (United States)

    Villeneuve, Julien; Scarpa, Margherita; Ortega-Bellido, Maria; Malhotra, Vivek

    2013-01-01

    The pericentriolar stacks of Golgi cisternae are separated from each other in G2 and fragmented extensively during mitosis. MEK1 is required for Golgi fragmentation in G2 and for the entry of cells into mitosis. We now report that Myt1 mediates MEK1's effects on the Golgi complex. Knockdown of Myt1 by siRNA increased the efficiency of Golgi complex fragmentation by mitotic cytosol in permeabilized and intact HeLa cells. Myt1 knockdown eliminated the requirement of MEK1 in Golgi fragmentation and alleviated the delay in mitotic entry due to MEK1 inhibition. The phosphorylation of Myt1 by MEK1 requires another kinase but is independent of RSK, Plk, and CDK1. Altogether our findings reveal that Myt1 is inactivated by MEK1 mediated phosphorylation to fragment the Golgi complex in G2 and for the entry of cells into mitosis. It is known that Myt1 inactivation is required for CDK1 activation. Myt1 therefore is an important link by which MEK1 dependent fragmentation of the Golgi complex in G2 is connected to the CDK1 mediated breakdown of Golgi into tubules and vesicles in mitosis.

  18. The Anaphase-Promoting Complex/Cyclosome Is Essential for Entry into Meiotic M-Phase.

    Science.gov (United States)

    Malhotra, Saurav; Vinod, Palakkad Krishnanunni; Mansfeld, Jörg; Stemmann, Olaf; Mayer, Thomas U

    2016-01-11

    Vertebrate immature oocytes are arrested at prophase of meiosis I (MI). Hormonal stimulation breaks this prophase-I arrest and induces re-entry into MI. The mechanism underlying meiotic resumption remains largely elusive. Here, we demonstrate that the anaphase-promoting complex/cyclosome (APC/C) in complex with Cdh1 has an unexpected function in meiosis in that it is essential for meiotic resumption. We identify the catalytic subunit of protein phosphatase 6 (PP6c) as the critical substrate whose APC/C(Cdh1)-mediated destruction is a prerequisite for the re-entry of immature Xenopus laevis oocytes into MI. Preventing PP6c destruction impairs activating autophosphorylation of Aurora A, a cell-cycle kinase critical for meiotic translation. Restoring meiotic translation rescues the meiotic resumption defect of Cdh1-depleted oocytes. Thus, our studies discover that the essential function of the APC/C in triggering cell-cycle transitions is not limited to M-phase exit but also applies to entry into meiotic M-phase, and identify a crucial APC/C-PP6c-Aurora A axis in the resumption of female meiosis.

  19. Safe cycling!

    CERN Multimedia

    Anaïs Schaeffer

    2012-01-01

    The HSE Unit will be running a cycling safety campaign at the entrances to CERN's restaurants on 14, 15 and 16 May. Pop along to see if they can persuade you to get back in the saddle!   With summer on its way, you might feel like getting your bike out of winter storage. Well, the HSE Unit has come up with some original ideas to remind you of some of the most basic safety rules. This year, the prevention campaign will be focussing on three themes: "Cyclists and their equipment", "The bicycle on the road", and "Other road users". This is an opportunity to think about the condition of your bike as well as how you ride it. From 14 to 16 May, representatives of the Swiss Office of Accident Prevention and the Touring Club Suisse will join members of the HSE Unit at the entrances to CERN's restaurants to give you advice on safe cycling (see box). They will also be organising three activity stands where you can test your knowle...

  20. Malaria vaccines and their potential role in the elimination of malaria

    Directory of Open Access Journals (Sweden)

    Greenwood Brian M

    2008-12-01

    Full Text Available Abstract Research on malaria vaccines is currently directed primarily towards the development of vaccines that prevent clinical malaria. Malaria elimination, now being considered seriously in some epidemiological situations, requires a different vaccine strategy, since success will depend on killing all parasites in the community in order to stop transmission completely. The feature of the life-cycles of human malarias that presents the greatest challenge to an elimination programme is the persistence of parasites as asymptomatic infections. These are an important source from which transmission to mosquitoes can occur. Consequently, an elimination strategy requires a community-based approach covering all individuals and not just those who are susceptible to clinical malaria. The progress that has been made in development of candidate malaria vaccines is reviewed. It is unlikely that many of these will have the efficacy required for complete elimination of parasites, though they may have an important role to play as part of future integrated control programmes. Vaccines for elimination must have a high level of efficacy in order to stop transmission to mosquitoes. This might be achieved with some pre-erythrocytic stage candidate vaccines or by targeting the sexual stages directly with transmission-blocking vaccines. An expanded malaria vaccine programme with such objectives is now a priority.

  1. Antifreeze life cycle assessment (LCA

    Directory of Open Access Journals (Sweden)

    Kesić Jelena

    2005-01-01

    Full Text Available Antifreeze based on ethylene glycol is a commonly used commercial product The classification of ethylene glycol as a toxic material increased the disposal costs for used antifreeze and life cycle assessment became a necessity. Life Cycle Assessment (LCA considers the identification and quantification of raw materials and energy inputs and waste outputs during the whole life cycle of the analyzed product. The objectives of LCA are the evaluation of impacts on the environment and improvements of processes in order to reduce and/or eliminate waste. LCA is conducted through a mathematical model derived from mass and energy balances of all the processes included in the life cycle. In all energy processes the part of energy that can be transformed into some other kind of energy is called exergy. The concept of exergy considers the quality of different types of energy and the quality of different materials. It is also a connection between energy and mass transformations. The whole life cycle can be described by the value of the total loss of exergy. The physical meaning of this value is the loss of material and energy that can be used. The results of LCA are very useful for the analyzed products and processes and for the determined conditions under which the analysis was conducted. The results of this study indicate that recycling is the most satisfactory solution for the treatment of used antifreeze regarding material and energy consumption but the re-use of antifreeze should not be neglected as a solution.

  2. Assessment of the ATV-1 Re-Entry Observation Campaign for Future Re-Entry Missions

    Science.gov (United States)

    Lips, T.; Lohle, S.; Marynowsky, T.; Rees, D.; Stenbeak-Nielsen, H. C.; Beks, M. L.; Hatton, J.

    2010-09-01

    This paper summarizes the midterm results of the currently ongoing ESA study “Assessment of the ATV-1 Reentry Observation Campaign for Future Re-entry Missions”. The primary objective of this study is to investigate the data obtained during a joint ESA/NASA airborne observation campaign of the destructive re-entry of ATV-1 Jules Verne which occurred on September 29, 2008. The presented results are focused on spectroscopic fragment characterization(material identification), frame-by-frame fragment tracking(manual and automatic) for various video recordings, 3D triangulation of the tracked fragments, and fragment propagation until complete demise or ground impact, including the actual size and location of the ATV-1 debris footprint. Fragment propagation analyses comprise also the derivation of aerodynamic fragment properties and potential delta velocities. These parameters are of high importance for the re-entry safety analysis for ATV-2 Johannes Kepler.

  3. Cow's milk protein elimination in autistic children: language ...

    African Journals Online (AJOL)

    Cow's milk protein elimination in autistic children: language, cognitive and ... The parents of first group were instructed to eliminate cow milk (CM) from the diet of their ... Autistic patients underwent a Childhood Autism Rating Scale (CARS) test.

  4. STUDY ON ELIMINATING FLUORESCENCE IN COTTON PULP WITH PERACETIC ACID

    Institute of Scientific and Technical Information of China (English)

    LixinXu; BaoguoSun

    2004-01-01

    This paper has studied processing conditions and the influence of every variable to the pulp when the fluorescence in cotton linter pulp is eliminated with peracetic acid. The suitable variables of the elimination of fluorescence, are found.

  5. STUDY ON ELIMINATING FLUORESCENCE IN COTTON PULP WITH PERACETIC ACID

    Institute of Scientific and Technical Information of China (English)

    Lixin Xu; Baoguo Sun

    2004-01-01

    This paper has studied processing conditions and the influence of every variable to the pulp when the fluorescence in cotton linter pulp is eliminated with peracetic acid. The suitable variables of the elimination of fluorescence. are found.

  6. New Hepatitis C Drugs Might Eliminate the Disease

    Science.gov (United States)

    ... https://medlineplus.gov/news/fullstory_164173.html New Hepatitis C Drugs Might Eliminate the Disease But not ... March 20, 2017 (HealthDay News) -- Newer treatments for hepatitis C appear to eliminate the virus in the ...

  7. Entry properties and entry inhibitors of a human H7N9 influenza virus.

    Directory of Open Access Journals (Sweden)

    Youhui Si

    Full Text Available The recently identified human infections with a novel avian influenza H7N9 virus in China raise important questions regarding possible risk to humans. However, the entry properties and tropism of this H7N9 virus were poorly understood. Moreover, neuraminidase inhibitor resistant H7N9 isolates were recently observed in two patients and correlated with poor clinical outcomes. In this study, we aimed to elucidate the entry properties of H7N9 virus, design and evaluate inhibitors for H7N9 virus entry. We optimized and developed an H7N9-pseudotyped particle system (H7N9pp that could be neutralized by anti-H7 antibodies and closely mimicked the entry process of the H7N9 virus. Avian, human and mouse-derived cultured cells showed high, moderate and low permissiveness to H7N9pp, respectively. Based on influenza virus membrane fusion mechanisms, a potent anti-H7N9 peptide (P155-185-chol corresponding to the C-terminal ectodomain of the H7N9 hemagglutinin protein was successfully identified. P155-185-chol demonstrated H7N9pp-specific inhibition of infection with IC50 of 0.19 µM. Importantly, P155-185-chol showed significant suppression of A/Anhui/1/2013 H7N9 live virus propagation in MDCK cells and additive effects with NA inhibitors Oseltamivir and Zanamivir. These findings expand our knowledge of the entry properties of the novel H7N9 viruses, and they highlight the potential for developing a new class of inhibitors targeting viral entry for use in the next pandemic.

  8. Lean for Government: Eliminating the Seven Wastes

    Science.gov (United States)

    Shepherd, Christena C.

    2012-01-01

    With shrinking budgets and a slow economy, it is becoming increasingly important for all government agencies to become more efficient. Citizens expect and deserve efficient and effective services from federal, state and local government agencies. One of the best methods to improve efficiency and eliminate waste is to institute the business process improvement methodologies known collectively as Lean; however, with reduced budgets, it may not be possible to train everyone in Lean or to engage the services of a trained consultant. It is possible, however, to raise awareness of the "Seven Wastes" of Lean in each employee, and encourage them to identify areas for improvement. Management commitment is vital to the success of these initiatives, and it is also important to develop the right metrics that will track the success of these changes.

  9. Absorption, distribution and elimination of drotaverine.

    Science.gov (United States)

    Magyar, K; Lengyel, M; Knoll, J

    1978-01-01

    14C-drotaverine [1(3',4'-diethoxybenzale)-6, 7-diethoxy-1, 2,3, 4-tetrahydroisoquinoline. HCl; No-SpaR] is well absorbed after subcutaneous and oral administration in mice. Its distribution is not specific. After intravenous administration the drug penetrates rapidly into every organ as indicated by whole body autoradiography. In the first hours the concentration of drotaverine was higher in the intestinal wall than in the other tissues. The concentrations of drotaverine in the organs decrease soon after administration and the drug is excreted mainly with the bile as beta-glucuronide; 60% of the dose was in the bile collected during 5 hours. During 96 hours of observation, 67% of the radioactivity administered was found in the stools while only 20% of it was eliminated with urine.

  10. Bell inequalities from variable elimination methods

    Energy Technology Data Exchange (ETDEWEB)

    Budroni, Costantino [Dresden Univ. (Germany). Naturwissenschaftlich-Technische Fakultaet; Cabello, Adan [Sevilla Univ. (Spain). Dept. de Fisica Aplicada II

    2013-07-01

    Complete sets of tight Bell inequalities are necessary and sufficient conditions for the existence of local hidden variable models describing a given measurement scenario. They are facets of Pitowsky's correlation polytope and are usually obtained from its extreme points by solving the hull problem. While there are algorithms that find all the facets of a given correlation polytope, the time required to compute them grows exponentially as the number of settings increases. This method has therefore been applied only to simple cases with a reduced number of settings. In this talk an alternative method based on a combination of algebraic results on extensions of measures and variable elimination methods, e.g., the Fourier-Motzkin method, is presented. Non-trivial cases where our method overcomes some of the computational difficulties associated with the hull problem are discussed.

  11. RESULTS of the "ELIMINATING NOISE" campaign

    CERN Multimedia

    SC Unit

    2008-01-01

    From 4 to 6 August, CERN’s nurses conducted a screening campaign entitled "Eliminating noise". This campaign was especially aimed at young people exposed to noise during their leisure hours (playing in a band, listening to MP3 players, attending concerts, etc.). In all, 166 people attended the infirmary, where they were able to receive personalised advice, documentation and, above all, a hearing test (audiogram). While the high attendance of people in the younger age category (18-30) was a success, their audiogram data were a cause for concern, with 24.5% showing abnormal results, hearing deficiencies which, we should remind you, are irreversible. It should be noted that such conditions are almost exclusively caused by noise exposure in a non-professional environment (leisure activities, music, etc.). This latest campaign confirms the harmful effects of noise on people’s hearing due to the absence or insufficiency of protective equipment during music-related activities; this further unde...

  12. RESULTS of the "ELIMINATING NOISE" campaign

    CERN Multimedia

    SC Unit

    2008-01-01

    From 4 to 6 August, CERN’s nurses conducted a screening campaign entitled "Eliminating noise". This campaign was especially aimed at young people exposed to noise during their leisure hours (playing in a band, listening to MP3 players, attending concerts, etc.). In all, 166 people attended the Infirmary, where they were able to receive personalised advice, documentation and, above all, a hearing test (audiogram). While the high attendance of people in the younger age category (18-30) was a success, their audiogram data were a cause for concern, with 24.5% showing abnormal results, hearing deficiencies which, we should remind you, are irreversible. It should be noted that such conditions are almost exclusively caused by noise exposure in a non-professional environment (leisure activities, music, etc.). This latest campaign confirms the harmful effects of noise on people’s hearing due to the absence or insufficiency of protective equipment during music-related activities; this further unde...

  13. Entry Properties and Entry Inhibitors of a Human H7N9 Influenza Virus

    OpenAIRE

    Youhui Si; Jianguo Li; Yuqiang Niu; Xiuying Liu; Lili Ren; Li Guo; Min Cheng; Hongli Zhou; Jianwei Wang; Qi Jin; Wei Yang

    2014-01-01

    The recently identified human infections with a novel avian influenza H7N9 virus in China raise important questions regarding possible risk to humans. However, the entry properties and tropism of this H7N9 virus were poorly understood. Moreover, neuraminidase inhibitor resistant H7N9 isolates were recently observed in two patients and correlated with poor clinical outcomes. In this study, we aimed to elucidate the entry properties of H7N9 virus, design and evaluate inhibitors for H7N9 virus e...

  14. Eliminating paediatric infections and keeping mothers alive

    Directory of Open Access Journals (Sweden)

    Gray G

    2012-11-01

    Full Text Available The global plan of reducing the number of new child HIV infections and a reduction in the number of HIV-related maternal deaths by 2015 will require inordinate political commitment and strengthening of health systems in Sub-Saharan Africa where the burden of HIV infections in pregnant women is the highest. Preventing HIV infection in women of child-bearing age and unwanted pregnancies in HIV-positive women forms the cornerstone of long-term control of paediatric HIV infections. To achieve the goal of eliminating paediatric HIV infection by 2015, health systems strengthening to address prevention of mother-to-child HIV transmission cascade attrition and focusing on the elimination of breastmilk transmission is critical. Understanding the pathogenesis of breastmilk transmission and the mechanisms by which antiretroviral therapy impacts on transmission through this compartment will drive future interventions. Identifying and retaining HIV-positive pregnant women in care and committed to long-term antiretroviral therapy will improve maternal outcomes and concomitant reductions in maternal mortality. Research assessing the natural history of HIV infection and long-term outcomes in women who interrupt antiretroviral therapy post-weaning is urgently required. Data on the outcome of women who opt to continue the long-term use of antiretroviral therapy after initiating therapy during pregnancy will determine future policy in countries considering option B+. The prevalence of antiretroviral resistance and impact on survival in infants who sero-convert whilst receiving neonatal prophylaxis, or are exposed to maternal HAART through breastmilk at a population level, are currently unknown. In addition to the provision of biomedical interventions, healthcare workers and policy makers must address the structural, cultural and community issues that impact on treatment uptake, adherence to medication and retention in care.

  15. Deciphering the spatio-temporal regulation of entry and progression through mitosis.

    Science.gov (United States)

    Gheghiani, Lilia; Gavet, Olivier

    2014-02-01

    Mitosis has been studied since the early 1880s as a key event of the cell division cycle where remarkable changes in cellular architecture take place and ultimately lead to an equal segregation of duplicated chromosomes into two daughter cells. A detailed description of the complex and highly ordered cellular events taking place is now available. Many regulators involved in key steps including entry into mitosis, nuclear envelope breakdown, microtubule (MT) spindle formation, and chromosome attachment, as well as mitotic exit and cytokinesis, have also been identified. However, understanding the precise spatio-temporal contribution of each regulator in the cell reorganization process has been technically challenging. This review will focus on a number of recent advances in our understanding of the spatial distribution of protein activities and the temporal regulation of their activation and inactivation during entry and progression through mitosis by the use of intramolecular Förster resonance energy transfer (FRET)-based biosensors.

  16. EXPERIMENTAL DEVICE OF THE HIGH HUMIDITY AIR ELECTROSTATIC ELIMINATOR

    Institute of Scientific and Technical Information of China (English)

    吴筱青; 何尉岚

    1990-01-01

    As for electrostatic elimination there are many means.But the high humidity air electrostatic eliminator has simple,efficient, lightproof and fireproof etc. advantages. Thus it is often used in some industrial enterprises. The aim of this paper is to introduce an experimental device of high humidity air electrostatic eliminator and its principle and experimental data.

  17. Access, entry and researcher-participant position

    DEFF Research Database (Denmark)

    Louw, Arnt Vestergaard

    2015-01-01

    it deals with the research findings that such a research design produced. As well as the methodological issues of researcher access, entry and participant position in the field, this article reports on the following questions: What kinds of implicit expectations of the students are embedded in the way...... the school introduces and initiates the programme? What kinds of effects does this have on the motivation of the students? How do the terms and professional language of the profession work on the individual students in including and excluding ways? These specific descriptions of classroom pedagogy, inspired...

  18. Radiation Database for Earth and Mars Entry

    Science.gov (United States)

    2008-11-17

    d3Πg − a3Πu (0:18;0:33) Fox- Herzberg e3Πg − a3Πu (0:15;0:35) Radiation Database for Earth and Mars Entry RTO-EN-AVT-162 8 - 15 affect a few J...structures of the spectra, namely in lines. This motivates the development of approximate models which do not have the high resolution structures but provide...Dec 1996. [2] L.C. Hartung. Predicting radiative heat transfer in thermo-chemical non-equilibrium flow- fields: theory and user’s manual for the

  19. Evolved Acceleration Guidance for Planetary Entry

    Science.gov (United States)

    Mease, K. D.; Leavitt, J. A.; Ferch, M.

    The U.S. Apollo and Shuttle programs have proven the viability and effectiveness of acceleration guidance. New capabilities have been developed to augment this basic concept. Algorithms have been designed for low lift to drag ratio vehicles and for mid to high lift to drag ratio vehicles. These algorithms have been tested for Mars landing and for earth entry of reusable launch vehicles and have performed well in both cases. Also the trajectory planner has been incorporated into a landing footprint generator that is designed for on-board use.

  20. Reference pricing with endogenous generic entry.

    OpenAIRE

    Kurt R. Brekke; Canta, Chiara; Straume, Odd Rune

    2015-01-01

    In this paper we study the effect of reference pricing on pharmaceutical prices and ex-penditures when generic entry is endogenously determined. We develop a Salop-type model where a brand-name producer competes with generic producers in terms of prices. In the market there are two types of consumers: (i) brand biased consumers who choose between brand-name and generic drugs, and (ii) brand neutral consumers who choose between the different generic drugs. We find that, for a given number of ...

  1. Female entrepreneurial networks and foreign market entry

    DEFF Research Database (Denmark)

    Rosenbaum, Gitte Ohrt

    2017-01-01

    The purpose of this paper is to explore the role of networks in the 116 foreign market entries (FMEs) of women-owned small businesses. A multiple case study based on semi-structured interviews with eight female entrepreneurs in the Danish fashion design industry. The results show that contrary to...... entrepreneurial ventures....... or compromising their work-life balance. The present study’s findings suggest that ICT has a much stronger role in the FME of firms than previously envisioned. The study’s findings also have important implications for policymakers and practitioners charged with promoting the international growth of female...

  2. Acute amiodarone promotes drift and early termination of spiral wave re-entry.

    Science.gov (United States)

    Nakagawa, Harumichi; Honjo, Haruo; Ishiguro, Yuko S; Yamazaki, Masatoshi; Okuno, Yusuke; Harada, Masahide; Takanari, Hiroki; Sakuma, Ichiro; Kamiya, Kaichiro; Kodama, Itsuo

    2010-07-01

    Intravenous application of amiodarone is commonly used in the treatment of life-threatening arrhythmias, but the underlying mechanism is not fully understood. The purpose of the present study is to investigate the acute effects of amiodarone on spiral wave (SW) re-entry, the primary organization machinery of ventricular tachycardia/fibrillation (VT/VF), in comparison with lidocaine. A two-dimensional ventricular myocardial layer was obtained from 24 Langendorff-perfused rabbit hearts, and epicardial excitations were analyzed by high-resolution optical mapping. During basic stimulation, amiodarone (5 microM) caused prolongation of action potential duration (APD) by 5.6%-9.1%, whereas lidocaine (15 microM) caused APD shortening by 5.0%-6.4%. Amiodarone and lidocaine reduced conduction velocity similarly. Ventricular tachycardias induced by DC stimulation in the presence of amiodarone were of shorter duration (sustained-VTs >30 s/total VTs: 2/58, amiodarone vs 13/52, control), whereas those with lidocaine were of longer duration (22/73, lidocaine vs 14/58, control). Amiodarone caused prolongation of VT cycle length and destabilization of SW re-entry, which is characterized by marked prolongation of functional block lines, frequent wavefront-tail interactions near the rotation center, and considerable drift, leading to its early annihilation via collision with anatomical boundaries. Spiral wave re-entry in the presence of lidocaine was more stabilized than in control. In the anisotropic ventricular myocardium, amiodarone destabilizes SW re-entry facilitating its early termination. Lidocaine, in contrast, stabilizes SW re-entry resulting in its persistence.

  3. Ezrin interacts with the SARS coronavirus Spike protein and restrains infection at the entry stage.

    Directory of Open Access Journals (Sweden)

    Jean Kaoru Millet

    Full Text Available BACKGROUND: Entry of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV and its envelope fusion with host cell membrane are controlled by a series of complex molecular mechanisms, largely dependent on the viral envelope glycoprotein Spike (S. There are still many unknowns on the implication of cellular factors that regulate the entry process. METHODOLOGY/PRINCIPAL FINDINGS: We performed a yeast two-hybrid screen using as bait the carboxy-terminal endodomain of S, which faces the cytosol during and after opening of the fusion pore at early stages of the virus life cycle. Here we show that the ezrin membrane-actin linker interacts with S endodomain through the F1 lobe of its FERM domain and that both the eight carboxy-terminal amino-acids and a membrane-proximal cysteine cluster of S endodomain are important for this interaction in vitro. Interestingly, we found that ezrin is present at the site of entry of S-pseudotyped lentiviral particles in Vero E6 cells. Targeting ezrin function by small interfering RNA increased S-mediated entry of pseudotyped particles in epithelial cells. Furthermore, deletion of the eight carboxy-terminal amino acids of S enhanced S-pseudotyped particles infection. Expression of the ezrin dominant negative FERM domain enhanced cell susceptibility to infection by SARS-CoV and S-pseudotyped particles and potentiated S-dependent membrane fusion. CONCLUSIONS/SIGNIFICANCE: Ezrin interacts with SARS-CoV S endodomain and limits virus entry and fusion. Our data present a novel mechanism involving a cellular factor in the regulation of S-dependent early events of infection.

  4. Ezrin Interacts with the SARS Coronavirus Spike Protein and Restrains Infection at the Entry Stage

    Science.gov (United States)

    Millet, Jean Kaoru; Kien, François; Cheung, Chung-Yan; Siu, Yu-Lam; Chan, Wing-Lim; Li, Huiying; Leung, Hiu-Lan; Jaume, Martial; Bruzzone, Roberto; Malik Peiris, Joseph S.; Altmeyer, Ralf Marius; Nal, Béatrice

    2012-01-01

    Background Entry of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) and its envelope fusion with host cell membrane are controlled by a series of complex molecular mechanisms, largely dependent on the viral envelope glycoprotein Spike (S). There are still many unknowns on the implication of cellular factors that regulate the entry process. Methodology/Principal Findings We performed a yeast two-hybrid screen using as bait the carboxy-terminal endodomain of S, which faces the cytosol during and after opening of the fusion pore at early stages of the virus life cycle. Here we show that the ezrin membrane-actin linker interacts with S endodomain through the F1 lobe of its FERM domain and that both the eight carboxy-terminal amino-acids and a membrane-proximal cysteine cluster of S endodomain are important for this interaction in vitro. Interestingly, we found that ezrin is present at the site of entry of S-pseudotyped lentiviral particles in Vero E6 cells. Targeting ezrin function by small interfering RNA increased S-mediated entry of pseudotyped particles in epithelial cells. Furthermore, deletion of the eight carboxy-terminal amino acids of S enhanced S-pseudotyped particles infection. Expression of the ezrin dominant negative FERM domain enhanced cell susceptibility to infection by SARS-CoV and S-pseudotyped particles and potentiated S-dependent membrane fusion. Conclusions/Significance Ezrin interacts with SARS-CoV S endodomain and limits virus entry and fusion. Our data present a novel mechanism involving a cellular factor in the regulation of S-dependent early events of infection. PMID:23185364

  5. Resveratrol and aspirin eliminate tetraploid cells for anticancer chemoprevention.

    Science.gov (United States)

    Lissa, Delphine; Senovilla, Laura; Rello-Varona, Santiago; Vitale, Ilio; Michaud, Mickaël; Pietrocola, Federico; Boilève, Alice; Obrist, Florine; Bordenave, Chloé; Garcia, Pauline; Michels, Judith; Jemaà, Mohamed; Kepp, Oliver; Castedo, Maria; Kroemer, Guido

    2014-02-25

    Tetraploidy constitutes a genomically metastable state that can lead to aneuploidy and genomic instability. Tetraploid cells are frequently found in preneoplastic lesions, including intestinal cancers arising due to the inactivation of the tumor suppressor adenomatous polyposis coli (APC). Using a phenotypic screen, we identified resveratrol as an agent that selectively reduces the fitness of tetraploid cells by slowing down their cell cycle progression and by stimulating the intrinsic pathway of apoptosis. Selective killing of tetraploid cells was observed for a series of additional agents that indirectly or directly stimulate AMP-activated protein kinase (AMPK) including salicylate, whose chemopreventive action has been established by epidemiological studies and clinical trials. Both resveratrol and salicylate reduced the formation of tetraploid or higher-order polyploid cells resulting from the culture of human colon carcinoma cell lines or primary mouse epithelial cells lacking tumor protein p53 (TP53, best known as p53) in the presence of antimitotic agents, as determined by cytofluorometric and videomicroscopic assays. Moreover, oral treatment with either resveratrol or aspirin, the prodrug of salicylate, repressed the accumulation of tetraploid intestinal epithelial cells in the Apc(Min/+) mouse model of colon cancer. Collectively, our results suggest that the chemopreventive action of resveratrol and aspirin involves the elimination of tetraploid cancer cell precursors.

  6. Implications of Plasmodium vivax Biology for Control, Elimination, and Research

    Science.gov (United States)

    Olliaro, Piero L.; Barnwell, John W.; Barry, Alyssa; Mendis, Kamini; Mueller, Ivo; Reeder, John C.; Shanks, G. Dennis; Snounou, Georges; Wongsrichanalai, Chansuda

    2016-01-01

    This paper summarizes our current understanding of the biology of Plasmodium vivax, how it differs from Plasmodium falciparum, and how these differences explain the need for P. vivax-tailored interventions. The article further pinpoints knowledge gaps where investments in research are needed to help identify and develop such specific interventions. The principal obstacles to reduce and eventually eliminate P. vivax reside in 1) its higher vectorial capacity compared with P. falciparum due to its ability to develop at lower temperature and over a shorter sporogonic cycle in the vector, allowing transmission in temperate zones and making it less sensitive to vector control measures that are otherwise effective on P. falciparum; 2) the presence of dormant liver forms (hypnozoites), sustaining multiple relapsing episodes from a single infectious bite that cannot be diagnosed and are not susceptible to any available antimalarial except primaquine, with routine deployment restricted by toxicity; 3) low parasite densities, which are difficult to detect with current diagnostics leading to missed diagnoses and delayed treatments (and protracted transmission), coupled with 4) transmission stages (gametocytes) occurring early in acute infections, before infection is diagnosed. PMID:27799636

  7. A Method of Eliminating Information Disclosure in View Publishing

    Institute of Scientific and Technical Information of China (English)

    LIU Guohua; GAO Shihong

    2006-01-01

    Although it is convenient to exchange data by publishing view, but it may disclose sensitive information. The problem of how to eliminate information disclosure becomes a core problem in the view publishing process. In order to eliminate information disclosure, deciding view security algorithm and eliminating information disclosure algorithm are proposed, and the validity of the algorithms are proved by experiment. The experimental results showing, deciding view security algorithm can decide the safety of a set of views under prior knowledge, and eliminating information disclosure algorithm can eliminate disclosure efficiently.

  8. OPEN-LOOP FOG SIGNAL TESTING AND WAVELET ELIMINATING NOISE

    Institute of Scientific and Technical Information of China (English)

    ZHUYun-zhao; WANGShun-ring; MIAOLing-juan; WANGBo

    2005-01-01

    An open-loop fiber optic gyro (FOG) testing system is designed. The noise characteristic of open-loop fiber optic gyro signals is analyzed. The wavelet eliminating noise method is discussed and compared with other methods, such as smoothing and low-pass filter methods. Results indicate that the wavelet eliminating noise method can satisfy the measuring demand of the FOG weak output signal with noise disturbing. The wavelet analysis method can efficiently eliminate the noise and reserve the information of the signal. The eliminating noise effect of using different wavelet base functions is compared. The effectiveness of multiresolution wavelet analyses of eliminating noise is proved by experimental results.

  9. 77 FR 72715 - Informal Entry Limit and Removal of a Formal Entry Requirement

    Science.gov (United States)

    2012-12-06

    ... increase of the informal entry limit is also consistent with one of the goals of the Beyond the Border... the policy seemed inconsistent with the recent Congressional decision to increase the ad valorem MPFs... this analysis, CBP cannot ascertain with any degree of certainty the specific monetary impacts...

  10. Entry, Descent, and Landing Operations Analysis for the Genesis Entry Capsule

    Science.gov (United States)

    Desai, Prasun N.; Lyons, Daniel T.

    2007-01-01

    On September 8, 2004, the Genesis spacecraft returned to Earth after spending 29 months about the sun-Earth libration point (L1) collecting solar wind particles. Four hours prior to Earth arrival, the sample return capsule containing the samples was released for entry and subsequent landing at the Utah Test and Training Range. This paper provides an overview of the entry, descent, and landing trajectory analysis that was performed during the mission operations phase leading up to final approach to Earth. The final orbit determination solution produced an inertial entry flight-path angle of -8.002 deg (which was the desired nominal value) with a 3-sigma error of +/-0.0274 deg (a third of the requirement). The operations effort accurately delivered the entry capsule to the desired landing site. The final landing location was 8.3 km from the target, and was well within the allowable landing area. Overall, the Earth approach operation procedures worked well and there were no issues (logistically or performance based) that arose. As a result, the process of targeting a capsule from deep space and accurately landing it on Earth was successfully demonstrated.

  11. The Hera Saturn Entry Probe Mission

    CERN Document Server

    Mousis, O; Spilker, T; Venkatapathy, E; Poncy, J; Frampton, R; Coustenis, A; Reh, K; Lebreton, J -P; Fletcher, L N; Hueso, R; Amato, M J; Colaprete, A; Ferri, F; Stam, D; Wurz, P; Atreya, S; Aslam, S; Banfield, D J; Calcutt, S; Fischer, G; Holland, A; Keller, C; Kessler, E; Leese, M; Levacher, P; Morse, A; Munoz, O; Renard, J -B; Sheridan, S; Schmider, F -X; Snik, F; Waite, J H; Bird, M; Cavalié, T; Deleuil, M; Fortney, J; Gautier, D; Guillot, T; Lunine, J I; Marty, B; Nixon, C; Orton, G S; Sanchez-Lavega, A

    2015-01-01

    The Hera Saturn entry probe mission is proposed as an M--class mission led by ESA with a contribution from NASA. It consists of one atmospheric probe to be sent into the atmosphere of Saturn, and a Carrier-Relay spacecraft. In this concept, the Hera probe is composed of ESA and NASA elements, and the Carrier-Relay Spacecraft is delivered by ESA. The probe is powered by batteries, and the Carrier-Relay Spacecraft is powered by solar panels and batteries. We anticipate two major subsystems to be supplied by the United States, either by direct procurement by ESA or by contribution from NASA: the solar electric power system (including solar arrays and the power management and distribution system), and the probe entry system (including the thermal protection shield and aeroshell). Hera is designed to perform in situ measurements of the chemical and isotopic compositions as well as the dynamics of Saturn's atmosphere using a single probe, with the goal of improving our understanding of the origin, formation, and ev...

  12. Mycobacteria entry and trafficking into endothelial cells.

    Science.gov (United States)

    Baltierra-Uribe, Shantal Lizbeth; García-Vásquez, Manuel de Jesús; Castrejón-Jiménez, Nayeli Shantal; Estrella-Piñón, Mayra Patricia; Luna-Herrera, Julieta; García-Pérez, Blanca Estela

    2014-09-01

    Endothelial cells are susceptible to infection by mycobacteria, but the endocytic mechanisms that mycobacteria exploit to enter host cells and their mechanisms of intracellular transport are completely unknown. Using pharmacological inhibitors, we determined that the internalization of Mycobacterium tuberculosis (MTB), Mycobacterium smegmatis (MSM), and Mycobacterium abscessus (MAB) is dependent on the cytoskeleton and is differentially inhibited by cytochalasin D, nocodazole, cycloheximide, wortmannin, and amiloride. Using confocal microscopy, we investigated their endosomal trafficking by analyzing Rab5, Rab7, LAMP-1, and cathepsin D. Our results suggest that MSM exploits macropinocytosis to enter endothelial cells and that the vacuoles containing these bacteria fuse with lysosomes. Conversely, the entry of MTB seems to depend on more than one endocytic route, and the observation that only a subset of the intracellular bacilli was associated with phagolysosomes suggests that these bacteria are able to inhibit endosomal maturation to persist intracellularly. The route of entry for MAB depends mainly on microtubules, which suggests that MAB uses a different trafficking pathway. However, MAB is also able to inhibit endosomal maturation and can replicate intracellularly. Together, these findings provide the first evidence that mycobacteria modulate proteins of host endothelial cells to enter and persist within these cells.

  13. Text Entry by Gazing and Smiling

    Directory of Open Access Journals (Sweden)

    Outi Tuisku

    2013-01-01

    Full Text Available Face Interface is a wearable prototype that combines the use of voluntary gaze direction and facial activations, for pointing and selecting objects on a computer screen, respectively. The aim was to investigate the functionality of the prototype for entering text. First, three on-screen keyboard layout designs were developed and tested (n=10 to find a layout that would be more suitable for text entry with the prototype than traditional QWERTY layout. The task was to enter one word ten times with each of the layouts by pointing letters with gaze and select them by smiling. Subjective ratings showed that a layout with large keys on the edge and small keys near the center of the keyboard was rated as the most enjoyable, clearest, and most functional. Second, using this layout, the aim of the second experiment (n=12 was to compare entering text with Face Interface to entering text with mouse. The results showed that text entry rate for Face Interface was 20 characters per minute (cpm and 27 cpm for the mouse. For Face Interface, keystrokes per character (KSPC value was 1.1 and minimum string distance (MSD error rate was 0.12. These values compare especially well with other similar techniques.

  14. Structural correlates of rotavirus cell entry.

    Directory of Open Access Journals (Sweden)

    Aliaa H Abdelhakim

    2014-09-01

    Full Text Available Cell entry by non-enveloped viruses requires translocation into the cytosol of a macromolecular complex--for double-strand RNA viruses, a complete subviral particle. We have used live-cell fluorescence imaging to follow rotavirus entry and penetration into the cytosol of its ∼ 700 Å inner capsid particle ("double-layered particle", DLP. We label with distinct fluorescent tags the DLP and each of the two outer-layer proteins and track the fates of each species as the particles bind and enter BSC-1 cells. Virions attach to their glycolipid receptors in the host cell membrane and rapidly become inaccessible to externally added agents; most particles that release their DLP into the cytosol have done so by ∼ 10 minutes, as detected by rapid diffusional motion of the DLP away from residual outer-layer proteins. Electron microscopy shows images of particles at various stages of engulfment into tightly fitting membrane invaginations, consistent with the interpretation that rotavirus particles drive their own uptake. Electron cryotomography of membrane-bound virions also shows closely wrapped membrane. Combined with high resolution structural information about the viral components, these observations suggest a molecular model for membrane disruption and DLP penetration.

  15. Novel heparan sulfate-binding peptides for blocking herpesvirus entry.

    Directory of Open Access Journals (Sweden)

    Pranay Dogra

    Full Text Available Human cytomegalovirus (HCMV infection can lead to congenital hearing loss and mental retardation. Upon immune suppression, reactivation of latent HCMV or primary infection increases morbidity in cancer, transplantation, and late stage AIDS patients. Current treatments include nucleoside analogues, which have significant toxicities limiting their usefulness. In this study we screened a panel of synthetic heparin-binding peptides for their ability to prevent CMV infection in vitro. A peptide designated, p5+14 exhibited ~ 90% reduction in murine CMV (MCMV infection. Because negatively charged, cell-surface heparan sulfate proteoglycans (HSPGs, serve as the attachment receptor during the adsorption phase of the CMV infection cycle, we hypothesized that p5+14 effectively competes for CMV adsorption to the cell surface resulting in the reduction in infection. Positively charged Lys residues were required for peptide binding to cell-surface HSPGs and reducing viral infection. We show that this inhibition was not due to a direct neutralizing effect on the virus itself and that the peptide blocked adsorption of the virus. The peptide also inhibited infection of other herpesviruses: HCMV and herpes simplex virus 1 and 2 in vitro, demonstrating it has broad-spectrum antiviral activity. Therefore, this peptide may offer an adjunct therapy for the treatment of herpes viral infections and other viruses that use HSPGs for entry.

  16. A transcriptional program mediating entry into cellular quiescence.

    Directory of Open Access Journals (Sweden)

    Helen Liu

    2007-06-01

    Full Text Available The balance of quiescence and cell division is critical for tissue homeostasis and organismal health. Serum stimulation of fibroblasts is well studied as a classic model of entry into the cell division cycle, but the induction of cellular quiescence, such as by serum deprivation (SD, is much less understood. Here we show that SS and SD activate distinct early transcriptional responses genome-wide that converge on a late symmetric transcriptional program. Several serum deprivation early response genes (SDERGs, including the putative tumor suppressor genes SALL2 and MXI1, are required for cessation of DNA synthesis in response to SD and induction of additional SD genes. SDERGs are coordinately repressed in many types of human cancers compared to their normal counterparts, and repression of SDERGs predicts increased risk of cancer progression and death in human breast cancers. These results identify a gene expression program uniquely responsive to loss of growth factor signaling; members of SDERGs may constitute novel growth inhibitors that prevent cancer.

  17. Analytic Development of a Reference Profile for the First Entry in a Skip Atmospheric Entry

    Science.gov (United States)

    Garcia-Llama, Eduardo

    2010-01-01

    This note shows that a feasible reference drag profile for the first entry portion of a skip entry can be generated as a polynomial expression of the velocity. The coefficients of that polynomial are found through the resolution of a system composed of m + 1 equations, where m is the degree of the drag polynomial. It has been shown that a minimum of five equations (m = 4) are required to establish the range and the initial and final conditions on velocity and flight path angle. It has been shown that at least one constraint on the trajectory can be imposed through the addition of one extra equation in the system, which must be accompanied by the increase in the degree of the drag polynomial. In order to simplify the resolution of the system of equations, the drag was considered as being a probability density function of the velocity, with the velocity as a distribution function of the drag. Combining this notion with the introduction of empirically derived constants, it has been shown that the system of equations required to generate the drag profile can be successfully reduced to a system of linear algebraic equations. For completeness, the resulting drag profiles have been flown using the feedback linearization method of differential geometric control as a guidance law with the error dynamics of a second order homogeneous equation in the form of a damped oscillator. Satisfactory results were achieved when the gains in the error dynamics were changed at a certain point along the trajectory that is dependent on the velocity and the curvature of the drag as a function of the velocity. Future work should study the capacity to update the drag profile in flight when dispersions are introduced. Also, future studies should attempt to link the first entry, as presented and controlled in this note, with a more standard control concept for the second entry, such as the Apollo entry guidance, to try to assess the overall skip entry performance. A guidance law that includes

  18. Orthopoxvirus species and strain differences in cell entry

    Energy Technology Data Exchange (ETDEWEB)

    Bengali, Zain; Satheshkumar, P.S. [Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-3210 (United States); Moss, Bernard, E-mail: bmoss@nih.gov [Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-3210 (United States)

    2012-11-25

    Vaccinia virus (VACV) enters cells by a low pH endosomal route or by direct fusion with the plasma membrane. We previously found differences in entry properties of several VACV strains: entry of WR was enhanced by low pH, reduced by bafilomycin A1 and relatively unaffected by heparin, whereas entry of IHD-J, Copenhagen and Elstree were oppositely affected. Since binding and entry modes may have been selected by specific conditions of in vitro propagation, we now examined the properties of three distinct, recently isolated cowpox viruses and a monkeypox virus as well as additional VACV and cowpox virus strains. The recent isolates were more similar to WR than to other VACV strains, underscoring the biological importance of endosomal entry by orthopoxviruses. Sequence comparisons, gene deletions and gene swapping experiments indicated that viral determinants, other than or in addition to the A26 and A25 'fusion-suppressor' proteins, impact entry properties.

  19. Mid-L/D Lifting Body Entry Demise Analysis

    Science.gov (United States)

    Ling, Lisa

    2017-01-01

    The mid-lift-to-drag ratio (mid-L/D) lifting body is a fully autonomous spacecraft under design at NASA for enabling a rapid return of scientific payloads from the International Space Station (ISS). For contingency planning and risk assessment for the Earth-return trajectory, an entry demise analysis was performed to examine three potential failure scenarios: (1) nominal entry interface conditions with loss of control, (2) controlled entry at maximum flight path angle, and (3) controlled entry at minimum flight path angle. The objectives of the analysis were to predict the spacecraft breakup sequence and timeline, determine debris survival, and calculate the debris dispersion footprint. Sensitivity analysis was also performed to determine the effect of the initial pitch rate on the spacecraft stability and breakup during the entry. This report describes the mid-L/D lifting body and presents the results of the entry demise and sensitivity analyses.

  20. APC/C and SCFcyclin F Constitute a Reciprocal Feedback Circuit Controlling S-Phase Entry

    Directory of Open Access Journals (Sweden)

    Rajarshi Choudhury

    2016-09-01

    Full Text Available The anaphase promoting complex/cyclosome (APC/C is an ubiquitin ligase and core component of the cell-cycle oscillator. During G1 phase, APC/C binds to its substrate receptor Cdh1 and APC/CCdh1 plays an important role in restricting S-phase entry and maintaining genome integrity. We describe a reciprocal feedback circuit between APC/C and a second ubiquitin ligase, the SCF (Skp1-Cul1-F box. We show that cyclin F, a cell-cycle-regulated substrate receptor (F-box protein for the SCF, is targeted for degradation by APC/C. Furthermore, we establish that Cdh1 is itself a substrate of SCFcyclin F. Cyclin F loss impairs Cdh1 degradation and delays S-phase entry, and this delay is reversed by simultaneous removal of Cdh1. These data indicate that the coordinated, temporal ordering of cyclin F and Cdh1 degradation, organized in a double-negative feedback loop, represents a fundamental aspect of cell-cycle control. This mutual antagonism could be a feature of other oscillating systems.

  1. Eliminating Tuberculosis One Neighborhood at a Time

    Science.gov (United States)

    Griffith, David E.; McGaha, Paul K.; Wolfgang, Melanie; Robinson, Celia B.; Clark, Patricia A.; Hassell, Willis L.; Robison, Valerie A.; Walker, Kerfoot P.; Wallace, Charles

    2014-01-01

    Objectives. We evaluated a strategy for preventing tuberculosis (TB) in communities most affected by it. Methods. In 1996, we mapped reported TB cases (1985–1995) and positive tuberculin skin test (TST) reactors (1993–1995) in Smith County, Texas. We delineated the 2 largest, densest clusters, identifying 2 highest-incidence neighborhoods (180 square blocks, 3153 residents). After extensive community preparation, trained health care workers went door-to-door offering TST to all residents unless contraindicated. TST-positive individuals were escorted to a mobile clinic for radiography, clinical evaluation, and isoniazid preventive treatment (IPT) as indicated. To assess long-term impact, we mapped all TB cases in Smith County during the equivalent time period after the project. Results. Of 2258 eligible individuals, 1291 (57.1%) were tested, 229 (17.7%) were TST positive, and 147 were treated. From 1996 to 2006, there were no TB cases in either project neighborhood, in contrast with the preintervention decade and the continued occurrence of TB in the rest of Smith County. Conclusions. Targeting high-incidence neighborhoods for active, community-based screening and IPT may hasten TB elimination in the United States. PMID:24899457

  2. [Elimination of all trans fatty acids].

    Science.gov (United States)

    Katan, M B

    2008-02-09

    At the start of the 20th century, the production of trans fatty acids was originally largely driven by the increasing demand for margarine. The two Dutch margarine firms Van den Bergh and Jurgens played an important role in this early development. In the early 1990s it was shown that trans fatty acids increase the risk of heart disease. Unilever, the successor to Van den Bergh and Jurgens, then took the lead in eliminating trans fatty acids from retail foods worldwide. As a result, intake in The Netherlands fell from 15 g per day in 1980 to 3 g per day in 2003. Dairy products and meat are now the major source of trans fatty acids. The effects on health of these ruminant trans fatty acids are unclear. There are three lessons to be learned from the rise and fall of trans fatty acids. First, a history of safe use does not guarantee safety of food components, because routine surveillance will fail to detect adverse effects on common illnesses with long incubation periods. Second, it shows that it is more effective and easier to change the composition of foods than to change consumer behaviour. And third, governments can have a major impact on consumers' health by mandating the use of healthier food ingredients.

  3. ON-LINE ELIMINATION OF OIL WHIP

    Institute of Scientific and Technical Information of China (English)

    Zheng Shuiying; Liu Shulian

    2005-01-01

    A new method to eliminate the oil whip online is put forward by use of passive electromagnetic damper. The damper works contactless and with DC current. Neither sensor nor closed loop control is needed. The dynamic equations of rotor-bearing system are built up by combining d'Alemdert principle with Rize way, and the nonlinear oil film forces based on unsteady short bearing model are coupled to system. Such nonlinear equations are numerically solved by Newmark integration method. The calculated results show that the bifurcation behavior of the system can be changed and the oil whip of the rotor may be well damped by external damping. The bifurcation diagrams also show that the subharmonic vibration amplitude decreases in motion and the speed at which the system losses its stability increases obviously by exerting external damping.Then experiments are carried out to demonstrate this phenomenon. It is observed that the complex trajectories of the journal motion are disappeared and the rotor-bearing system became stable when the power of passive electromagnetic damper is turned on. The experiments have good repeatability.

  4. Eliminating poverty will help women most.

    Science.gov (United States)

    Short, C

    1997-01-01

    It is both possible and affordable to eliminate poverty. It is also necessary to promote peaceful existence in the next century and to reduce population pressure. A key to poverty reduction is to increase equality between men and women. Women must be given access to reproductive health services and care so that they can bear their children safely and protect themselves from infection. The maternal mortality risk in Africa far exceeds that in developed countries, and many more women suffer permanent disability from child birth. At least 120 million have an unmet need for contraception, and more than 90% of AIDS cases occur among the impoverished and marginalized population of developing countries. In addition to meeting the reproductive health needs of men and women, the needs of youth must be met through coherent and coordinated efforts that support the work of all partners in development according to an agreed upon agenda crafted by developing countries. The priorities must be to 1) increase access to reproductive health information, services, and commodities and 2) to minimize the need for abortion while recognizing that women should be able to make their own moral choices and to obtain a safe abortion. The goals of the international development community for 2015 are to halve the proportion of people living in extreme poverty, to ensure reproductive health for all, and to achieve a 75% reduction in maternal mortality.

  5. Malaria vaccine: a step toward elimination.

    Science.gov (United States)

    Jindal, Harashish; Bhatt, Bhumika; Malik, Jagbir S; Sk, Shashikantha; Mehta, Bharti

    2014-01-01

    Malaria has long been recognized as a public health problem. At the community level, vector control, and antimalarial medicines are the main means for reducing incidence, morbidity, and mortality of malaria. A vaccine not only would bring streamlining in the prevention of morbidity and mortality from malaria but also would be more accessible if integrated with Expanded Programme of Immunization (EPI). Globally, an estimated 3.4 billion people are at risk of malaria. Most cases (80%) and deaths (90%) occurred in Africa, and most deaths (77%) are in children under 5 years of age. An effective vaccine has long been envisaged as a valuable addition to the available tools for malaria control. Although research toward the development of malaria vaccines has been pursued since the 1960s, there are no licensed malaria vaccines. The RTS,S/AS01 vaccine, which targets P. falciparum, has reached phase 3 clinical trials and results are promising. Malaria Vaccine Technology Road Map 2013 has envisaged the world aiming for a licensed vaccine by 2030 that would reduce malaria cases by 75% and be capable of eliminating malaria. It will not only fill the gaps of today's interventions but also be a cost-effective method of decreasing morbidity and mortality from malaria.

  6. Environmental issues elimination through circular economy

    Science.gov (United States)

    Špirková, M.; Pokorná, E.; Šujanová, J.; Samáková, J.

    2016-04-01

    Environmental efforts of European Union are currently going towards circular economy. Tools like Extended Producer Responsibility and Eco-design were established. The circular economy deals with resources availability issue on one hand and waste management on the other hand. There are few pioneering companies all over the world with some kind of circular economy practice. Generally the concept is not very wide-spread. The paper aims to evaluate possibility of transition towards circular economy in Slovak industrial companies. They need to have an active approach to material treatment of their products after usage stage. Innovation is another important pre-condition for the transition. Main problem of current cradle to grave system is landfilling of valuable materials after one cycle of usage. Their potential value for next manufacturing cycles is lost. Companies may do not see connection between waste management and material resource prices and volatility of supplies. Municipalities are responsible for municipal waste collection and treatment in Slovakia. The circular economy operates by cradle to cradle principle. Company manages material flow until the material comes back to the beginning of manufacturing process by itself or by another partners. Stable material supplies with quite low costs are provided this way. It is necessary to deal with environmental problems in phase of product design. Questionnaire survey results show on one hand low involvement of industrial companies in waste management area, however on the other hand they are open to environmental innovations in future.

  7. Environmental issues elimination through circular economy

    Energy Technology Data Exchange (ETDEWEB)

    Špirková, M., E-mail: marta.spirkova@stuba.sk; Pokorná, E.; Šujanová, J.; Samáková, J. [Paulínska 16, 917 24 Trnava, Slovakia, Slovak University of Technology in Bratislava, Faculty of Materials Science and Technology in Trnava (Slovakia)

    2016-04-21

    Environmental efforts of European Union are currently going towards circular economy. Tools like Extended Producer Responsibility and Eco-design were established. The circular economy deals with resources availability issue on one hand and waste management on the other hand. There are few pioneering companies all over the world with some kind of circular economy practice. Generally the concept is not very wide-spread. The paper aims to evaluate possibility of transition towards circular economy in Slovak industrial companies. They need to have an active approach to material treatment of their products after usage stage. Innovation is another important pre-condition for the transition. Main problem of current cradle to grave system is landfilling of valuable materials after one cycle of usage. Their potential value for next manufacturing cycles is lost. Companies may do not see connection between waste management and material resource prices and volatility of supplies. Municipalities are responsible for municipal waste collection and treatment in Slovakia. The circular economy operates by cradle to cradle principle. Company manages material flow until the material comes back to the beginning of manufacturing process by itself or by another partners. Stable material supplies with quite low costs are provided this way. It is necessary to deal with environmental problems in phase of product design. Questionnaire survey results show on one hand low involvement of industrial companies in waste management area, however on the other hand they are open to environmental innovations in future.

  8. Combined rankine and vapor compression cycles

    Science.gov (United States)

    Radcliff, Thomas D.; Biederman, Bruce P.; Brasz, Joost J.

    2005-04-19

    An organic rankine cycle system is combined with a vapor compression cycle system with the turbine generator of the organic rankine cycle generating the power necessary to operate the motor of the refrigerant compressor. The vapor compression cycle is applied with its evaporator cooling the inlet air into a gas turbine, and the organic rankine cycle is applied to receive heat from a gas turbine exhaust to heat its boiler within one embodiment, a common condenser is used for the organic rankine cycle and the vapor compression cycle, with a common refrigerant, R-245a being circulated within both systems. In another embodiment, the turbine driven generator has a common shaft connected to the compressor to thereby eliminate the need for a separate motor to drive the compressor. In another embodiment, an organic rankine cycle system is applied to an internal combustion engine to cool the fluids thereof, and the turbo charged air is cooled first by the organic rankine cycle system and then by an air conditioner prior to passing into the intake of the engine.

  9. Energy demand for elimination of organic micropollutants in municipal wastewater treatment plants.

    Science.gov (United States)

    Mousel, Danièle; Palmowski, Laurence; Pinnekamp, Johannes

    2017-01-01

    Organic micropollutants (OMP), e.g. pharmaceuticals and household/industrial chemicals, are not fully eliminated in state-of-the-art municipal wastewater treatment plants and can potentially harm the aquatic environment. Therefore, several pilot and large-scale investigations on the elimination of organic micropollutants have taken place in recent years. Based on the present findings, the most efficient treatment steps to eliminate organic micropollutants have proven to be ozonation, adsorption on powdered activated carbon (PAC), or filtration through granular activated carbon (GAC). Yet a further treatment step implies an increase in energy demand of the wastewater treatment plant, which has to be considered along with OMP elimination. To this aim, data on energy demand of ten large-scale municipal wastewater treatment plants (WWTP) with processes for OMP elimination was collected and analyzed. Moreover, calculations on energy demand beyond the WWTP for production and transport of ancillary materials were performed to assess the cumulative energy demand of the processes. An assessment of the greenhouse gas emissions of the processes was achieved, which shall facilitate future life cycle analyses. The results show that energy demand of ozonation at the wastewater treatment plant is dependent upon the ozone dosage and is significantly higher than energy demand of PAC addition or GAC filtration (2 to 4 times higher without consideration of delivery heads). Despite uncertainties regarding the energy demand for production of activated carbon, it could be shown that the cumulative energy demand of adsorption steps is significantly higher than the energy demand at the WWTP. Using reactivated GAC can lead to energy and greenhouse gas emissions savings compared to using fresh GAC/PAC. Moreover, energy demand is always plant-specific and depends on different factors (delivery heads, existing filtration or post-treatment etc.). Since processes for elimination of organic

  10. Aspirin inhibits hepatitis C virus entry by downregulating claudin-1.

    Science.gov (United States)

    Yin, P; Zhang, L

    2016-01-01

    Aspirin has previously been reported to inhibit hepatitis C virus (HCV) replication. The aim of this study was to investigate whether aspirin is involved in blocking HCV entry. We found that aspirin inhibits the entry of HCVpp and infectious HCV. The level of claudin-1, an HCV receptor, is reduced by aspirin. Our results extend the anti-HCV effect of aspirin to the HCV entry step and further reinforce the anti-HCV role of aspirin.

  11. The effects of the diurnal atmospheric variability on entry, descent and landing on Mars

    Directory of Open Access Journals (Sweden)

    Marčeta D.

    2014-01-01

    Full Text Available Landing on Mars is extremely challenging task due to the fact that the Martian atmosphere is the most hostile environment in the Solar system to perform the entry, descent and landing (EDL process, because it is thick enough to create substantial heating of the entry vehicle but not thick enough to reduce its velocity to the one necessary for safe landing. Beside this, the atmosphere is very dynamic mainly due to high eccentricity of the Martian orbit, obliquity of the orbital to the equatorial plane and close alignment of the winter solstice and the orbital perihelion. Although seasonal variations of atmospheric parameters are significantly larger than the diurnal, it is very important to analyze diurnal cycles as they can significantly change vertical and horizontal atmospheric profiles in very short time intervals. This can present a serious threat to missions which have very precise timings and specific requirements such as the requirement for the daytime landing to enable ground images acquisition during the descent and landing phase. A 3-degrees-of-freedom trajectory integration routine was combined with the Mars Global Reference Atmospheric Model (Mars-GRAM to identify the dependence of the EDL profiles on the diurnal cycles of atmospheric parameters throughout the Martian year. The obtained results show that the influence of the diurnal cycles is the largest at the equator and decreases relatively symmetrically towards the poles with a slightly stronger influence in the northern hemisphere. Also, there is a significant influence of the orbital position of Mars on the effect of diurnal atmospheric variations which causes that, around the orbital perihelion and winter solstice, there is some kind of inversion of the dependance of optimal entry timing on latitude of the landing site comparing to the rest of the Martian year. [Projekat Ministarstva nauke Republike Srbije, br. 176002

  12. An Integrated Approach for Entry Mission Design and Flight Simulations

    Science.gov (United States)

    Lu, Ping; Rao, Prabhakara

    2004-01-01

    An integrated approach for entry trajectory design, guidance, and simulation is proposed. The key ingredients for this approach are an on-line 3 degree-of-freedom entry trajectory planning algorithm and the entry guidance algorithm that generates the guidance gains automatically. When fully developed, such a tool could enable end-bend entry mission design and simulations in 3DOF and 6DOF mode from de-orbit burn to the TAEM interface and beyond, all in one key stroke. Some preliminary examples of such a capability are presented in this paper that demonstrate the potential of this type of integrated environment.

  13. A novel peptide that inhibits HIV-1 entry

    Institute of Scientific and Technical Information of China (English)

    YU Yong; HUANG Xiaoxing; WANG Qiong; YANG Yaling; TIAN Po; ZHANG Wentao

    2004-01-01

    @@ The global epidemic of HIV infection, the cause of AIDS, has created an urgent need for novel classes of antiretroviral agent. Besides reverse transcriptase and protease, the viral entry process provides new anti-HIV-1 targets. A new generation of antiviral drugs intended to block HIV entry into host cells is now under develop- ment[1]. These compounds are generally referred to as fusion or entry inhibitor. Several HIV-1 entry inhibitors that target CD4-gp120 interactions, co-receptor function, and gp41-mediated membrane fusion are in different stages of clinical development[2].

  14. Protease Inhibitors Targeting Coronavirus and Filovirus Entry

    Science.gov (United States)

    Zhou, Yanchen; Vedantham, Punitha; Lu, Kai; Agudelo, Juliet; Carrion, Ricardo; Nunneley, Jerritt W.; Barnard, Dale; Pöhlmann, Stefan; McKerrow, James H.; Renslo, Adam R.; Simmons, Graham

    2016-01-01

    In order to gain entry into cells, diverse viruses, including Ebola virus, SARS-coronavirus and the emerging MERS-coronavirus, depend on activation of their envelope glycoproteins by host cell proteases. The respective enzymes are thus excellent targets for antiviral intervention. In cell culture, activation of Ebola virus, as well as SARS- and MERS-coronavirus can be accomplished by the endosomal cysteine proteases, cathepsin L (CTSL) and cathepsin B (CTSB). In addition, SARS- and MERS-coronavirus can use serine proteases localized at the cell surface, for their activation. However, it is currently unclear which protease(s) facilitate viral spread in the infected host. We report here that the cysteine protease inhibitor K11777, ((2S)-N-[(1E,3S)-1-(benzenesulfonyl)-5-phenylpent-1-en-3-yl]-2-{[(E)-4-methylpiperazine-1-carbonyl]amino}-3-phenylpropanamide) and closely-related vinylsulfones act as broad-spectrum antivirals by targeting cathepsin-mediated cell entry. K11777 is already in advanced stages of development for a number of parasitic diseases, such as Chagas disease, and has proven to be safe and effective in a range of animal models. K11777 inhibition of SARS-CoV and Ebola virus entry was observed in the sub-nanomolar range. In order to assess, whether cysteine or serine proteases promote viral spread in the host, we compared the antiviral activity of an optimized K11777-derivative with that of camostat, an inhibitor of TMPRSS2 and related serine proteases. Employing a pathogenic animal model of SARS-CoV infection, we demonstrated that viral spread and pathogenesis of SARS-CoV is driven by serine rather than cysteine proteases and can be effectively prevented by camostat. Camostat has been clinically used to treat chronic pancreatitis, and thus represents an exciting potential therapeutic for respiratory coronavirus infections. Our results indicate that camostat, or similar serine protease inhibitors, might be an effective option for treatment of SARS and

  15. Heatshield for Extreme Entry Environment Technology (HEEET)

    Science.gov (United States)

    Venkatapathy, E.; Ellerby, D.; Stackpoole, M..; Peterson, K.; Gage, P.; Beerman, A.; Blosser, M.; Chinnapongse, R.; Dillman, R.; Feldman, J.; Gasch, M.; Munk, M.; Prabhu, D.; Poteet, C.

    2013-01-01

    Heat-shield for Extreme Entry Technology (HEEET) project is based on the 3-D Woven TPS, an emerging innovative and game changing technology funded by SMD and STMD to fill the ablative TPS gap that exists currently for reaching the depths of Saturn and Venus. Woven TPS technology will address the challenges currently faced by the Venus, Saturn, and higher speed sample return mission Science community due to lack of availability of the only TPS, namely Carbon Phenolic and enable the Science community to move forward with proposals in this decade with Woven TPS. This presentation describes the approach in maturing the technology in the next three years enabling NF-4 mission proposers to address the challenges of Venus, Saturn or higher speed sample return missions.

  16. Lattice Boltzmann modeling of water entry problems

    Science.gov (United States)

    Zarghami, A.; Falcucci, G.; Jannelli, E.; Succi, S.; Porfiri, M.; Ubertini, S.

    2014-12-01

    This paper deals with the simulation of water entry problems using the lattice Boltzmann method (LBM). The dynamics of the free surface is treated through the mass and momentum fluxes across the interface cells. A bounce-back boundary condition is utilized to model the contact between the fluid and the moving object. The method is implemented for the analysis of a two-dimensional flow physics produced by a symmetric wedge entering vertically a weakly-compressible fluid at a constant velocity. The method is used to predict the wetted length, the height of water pile-up, the pressure distribution and the overall force on the wedge. The accuracy of the numerical results is demonstrated through comparisons with data reported in the literature.

  17. Are entry criteria for cataract surgery justified?

    Directory of Open Access Journals (Sweden)

    Daniel Böhringer

    Full Text Available PURPOSE: The German Ophthalmological Society (GOS recently proposed surgical entry criteria, i.e. 300 cataract surgeries. We herein correlate the surgical hands-on experience with the risk of posterior capsule ruptures in order to assess whether this number is appropriate. METHODS: We identified all cataract operations that had been performed at the University Eye Hospital Freiburg since 1995. For each surgeon, we assigned a running number to his/her procedures in the order they had been performed. Thereafter, we excluded all combined procedures and the second eyes. We then selected the 5475 surgical reports between November 2008 and November 2012 for detailed review. We additionally classified each surgery into low- vs. high- à priori risk for posterior capsule ruptures. We fitted a multifactorial logistic regression model to assess the GOS recommendation of 300 surgeries under supervision. In the low-risk group, we additionally visualized the 'typical' learning curve by plotting the posterior capsule ruptures against the respective rank numbers. RESULTS: The odds ratio for posterior capsule ruptures of 'learning-mode' (one of the respective surgeon's 300 first procedures vs. the non-learning-mode was 3.8 (p<0.0001. By contrast, classification into the low-risk group lowered the risk of posterior capsule ruptures three fold (p<0.0001. According to the low-risk plot, the surgeons started with a complication rate of 4% and continuously improved towards 0.5% after 1500 operations. Thereafter, the rate increased again and stabilized around one percent. CONCLUSION: The learning curve with respect to posterior capsule ruptures is surprisingly flat. The GOS entry criterion of 300 cataract procedures is therefore most likely justified. Careful selection of low-risk patients for the training surgeons may help in reducing the rate of posterior capsule ruptures during training.

  18. The Hera Saturn entry probe mission

    Science.gov (United States)

    Mousis, O.; Atkinson, D. H.; Spilker, T.; Venkatapathy, E.; Poncy, J.; Frampton, R.; Coustenis, A.; Reh, K.; Lebreton, J.-P.; Fletcher, L. N.; Hueso, R.; Amato, M. J.; Colaprete, A.; Ferri, F.; Stam, D.; Wurz, P.; Atreya, S.; Aslam, S.; Banfield, D. J.; Calcutt, S.; Fischer, G.; Holland, A.; Keller, C.; Kessler, E.; Leese, M.; Levacher, P.; Morse, A.; Muñoz, O.; Renard, J.-B.; Sheridan, S.; Schmider, F.-X.; Snik, F.; Waite, J. H.; Bird, M.; Cavalié, T.; Deleuil, M.; Fortney, J.; Gautier, D.; Guillot, T.; Lunine, J. I.; Marty, B.; Nixon, C.; Orton, G. S.; Sánchez-Lavega, A.

    2016-10-01

    The Hera Saturn entry probe mission is proposed as an M-class mission led by ESA with a contribution from NASA. It consists of one atmospheric probe to be sent into the atmosphere of Saturn, and a Carrier-Relay spacecraft. In this concept, the Hera probe is composed of ESA and NASA elements, and the Carrier-Relay Spacecraft is delivered by ESA. The probe is powered by batteries, and the Carrier-Relay Spacecraft is powered by solar panels and batteries. We anticipate two major subsystems to be supplied by the United States, either by direct procurement by ESA or by contribution from NASA: the solar electric power system (including solar arrays and the power management and distribution system), and the probe entry system (including the thermal protection shield and aeroshell). Hera is designed to perform in situ measurements of the chemical and isotopic compositions as well as the dynamics of Saturn's atmosphere using a single probe, with the goal of improving our understanding of the origin, formation, and evolution of Saturn, the giant planets and their satellite systems, with extrapolation to extrasolar planets. Hera's aim is to probe well into the cloud-forming region of the troposphere, below the region accessible to remote sensing, to the locations where certain cosmogenically abundant species are expected to be well mixed. By leading to an improved understanding of the processes by which giant planets formed, including the composition and properties of the local solar nebula at the time and location of giant planet formation, Hera will extend the legacy of the Galileo and Cassini missions by further addressing the creation, formation, and chemical, dynamical, and thermal evolution of the giant planets, the entire solar system including Earth and the other terrestrial planets, and formation of other planetary systems.

  19. Coupling approaches used in atmospheric entry models

    Science.gov (United States)

    Gritsevich, M. I.

    2012-09-01

    While a planet orbits the Sun, it is subject to impact by smaller objects, ranging from tiny dust particles and space debris to much larger asteroids and comets. Such collisions have taken place frequently over geological time and played an important role in the evolution of planets and the development of life on the Earth. Though the search for near-Earth objects addresses one of the main points of the Asteroid and Comet Hazard, one should not underestimate the useful information to be gleaned from smaller atmospheric encounters, known as meteors or fireballs. Not only do these events help determine the linkages between meteorites and their parent bodies; due to their relative regularity they provide a good statistical basis for analysis. For successful cases with found meteorites, the detailed atmospheric path record is an excellent tool to test and improve existing entry models assuring the robustness of their implementation. There are many more important scientific questions meteoroids help us to answer, among them: Where do these objects come from, what are their origins, physical properties and chemical composition? What are the shapes and bulk densities of the space objects which fully ablate in an atmosphere and do not reach the planetary surface? Which values are directly measured and which are initially assumed as input to various models? How to couple both fragmentation and ablation effects in the model, taking real size distribution of fragments into account? How to specify and speed up the recovery of a recently fallen meteorites, not letting weathering to affect samples too much? How big is the pre-atmospheric projectile to terminal body ratio in terms of their mass/volume? Which exact parameters beside initial mass define this ratio? More generally, how entering object affects Earth's atmosphere and (if applicable) Earth's surface? How to predict these impact consequences based on atmospheric trajectory data? How to describe atmospheric entry

  20. Passive Earth Entry Vehicle Landing Test

    Science.gov (United States)

    Kellas, Sotiris

    2017-01-01

    Two full-scale passive Earth Entry Vehicles (EEV) with realistic structure, surrogate sample container, and surrogate Thermal Protection System (TPS) were built at NASA Langley Research Center (LaRC) and tested at the Utah Test and Training Range (UTTR). The main test objective was to demonstrate structural integrity and investigate possible impact response deviations of the realistic vehicle as compared to rigid penetrometer responses. With the exception of the surrogate TPS and minor structural differences in the back shell construction, the two test vehicles were identical in geometry and both utilized the Integrated Composite Stiffener Structure (ICoSS) structural concept in the forward shell. The ICoSS concept is a lightweight and highly adaptable composite concept developed at NASA LaRC specifically for entry vehicle TPS carrier structures. The instrumented test vehicles were released from a helicopter approximately 400 m above ground. The drop height was selected such that at least 98% of the vehicles terminal velocity would be achieved. While drop tests of spherical penetrometers and a low fidelity aerodynamic EEV model were conducted at UTTR in 1998 and 2000, this was the first time a passive EEV with flight-like structure, surrogate TPS, and sample container was tested at UTTR for the purpose of complete structural system validation. Test results showed that at a landing vertical speed of approximately 30 m/s, the test vehicle maintained structural integrity and enough rigidity to penetrate the sandy clay surface thus attenuating the landing load, as measured at the vehicle CG, to less than 600 g. This measured deceleration was found to be in family with rigid penetrometer test data from the 1998 and 2000 test campaigns. Design implications of vehicle structure/soil interaction with respect to sample container and sample survivability are briefly discussed.

  1. Fast Simulation of Nonlinear Circuits with an Algorithm of Partial Matrix Elimination (PME)

    Institute of Scientific and Technical Information of China (English)

    CAI Xingjian; MAO Junfa; YANG Xiaoping; LI Zhengfan

    2001-01-01

    In this paper a theorem is proven thatthe contribution of any linear circuit elements to theadmittance matrix [Y] in the modified nodal admit-tance (MNA) equation of circuit analysis is invariantwith time.Based on this theorem a fast time-domainalgorithm of partial matrix elimination (PME) is de-veloped for simulation of high-speed nonlinear circuitswith interconnects.In PME the matrix [Y] is ar-ranged to consist of two submatrices.The lower sub-matrix that contains entries from only linear circuit el-ements is partially triangulated by Gauss eliminationfor the first-time-step transient analysis.At the fol-lowing time steps of transient analysis,Gauss elimina-tion is needed only for the upper submatrix containingcontributions of nonlinear circuit devices.Efficiencyanalysis of PME is given in detail,showing that it canaccelerate circuit simulation by a factor of at least 2.5for linear or lightly nonlinear circuits without employ-ing any numerical approximation.Moreover,PME isuseful for circuits debugging.

  2. [Plan for the Elimination of Measles and Rubella in Spain].

    Science.gov (United States)

    Limia Sánchez, Aurora

    2015-01-01

    The current Plan for the elimination of measles and rubella is made of the Plan for the elimination of measles in Spain, set up in 2001, and the Surveillance protocol of rubella and congenital rubella syndrome in the phase of elimination, established in 2007. The monitoring of the elimination of both diseases conducted from the European Region of World Health Organization (WHO) has been reinforced since 2010. An annual report with information on the situation of measles and rubella in The Member Estates is being requested since 2013. The Ministry of Health, Social Services and Equality nominated a National Committee for Verification of Measles and Rubella Elimination in 2013, for the review and delivery of the requested information. The strategies and activities currently recommended from the WHO European Region, the verification process in Spain, and the steps to follow for the updating of the Plan for the elimination of measles and rubella in Spain are mentioned in this article.

  3. Interplay among cellular polarization, lipoprotein metabolism and hepatitis C virus entry

    Institute of Scientific and Technical Information of China (English)

    Ignacio Benedicto; Francisca Molina-Jiménez; Ricardo Moreno-Otero; Manuel López-Cabrera; Pedro L Majano

    2011-01-01

    Hepatitis C virus (HCV) infects more than three million new individuals worldwide each year. In a high percentage of patients, acute infections become chronic, eventually progressing to fibrosis, cirrhosis, and hepatocellular carcinoma. Given the lack of effective prophylactic or therapeutic vaccines, and the limited sustained virological response rates to current therapies, new approaches are needed to prevent, control, and clear HCV infection.Entry into the host cell, being the first step of the viral cycle, is a potential target for the design of new antiviral compounds. Despite the recent discovery of the tight junction-associated proteins claudin-1 and occludin as HCV co-receptors, which is an important step towards the understanding of HCV entry, the precise mechanisms are still largely unknown. In addition, increasing evidence indicates that tools that are broadly employed to study HCV infection do not accurately reflect the real process in terms of viral particle composition and host cell phenotype.Thus, systems that more closely mimic natural infection are urgently required to elucidate the mechanisms of HCV entry, which will in turn help to design antiviral strategies against this part of the infection process.

  4. Acid phosphatase 2 (ACP2) is required for membrane fusion during influenza virus entry

    Science.gov (United States)

    Lee, Jihye; Kim, Jinhee; Son, Kidong; d’Alexandry d’Orengiani, Anne-Laure Pham Humg; Min, Ji-Young

    2017-01-01

    Influenza viruses exploit host factors to successfully replicate in infected cells. Using small interfering RNA (siRNA) technology, we identified six human genes required for influenza A virus (IAV) replication. Here we focused on the role of acid phosphatase 2 (ACP2), as its knockdown showed the greatest inhibition of IAV replication. In IAV-infected cells, depletion of ACP2 resulted in a significant reduction in the expression of viral proteins and mRNA, and led to the attenuation of virus multi-cycle growth. ACP2 knockdown also decreased replication of seasonal influenza A and B viruses and avian IAVs of the H7 subtype. Interestingly, ACP2 depletion had no effect on the replication of Ebola or hepatitis C virus. Because ACP2 is known to be a lysosomal acid phosphatase, we assessed the role of ACP2 in influenza virus entry. While neither binding of the viral particle to the cell surface nor endosomal acidification was affected in ACP2-depleted cells, fusion of the endosomal and viral membranes was impaired. As a result, downstream steps in viral entry were blocked, including nucleocapsid uncoating and nuclear import of viral ribonucleoproteins. Our results established ACP2 as a necessary host factor for regulating the fusion step of influenza virus entry. PMID:28272419

  5. Interaction of Human Tumor Viruses with Host Cell Surface Receptors and Cell Entry

    Directory of Open Access Journals (Sweden)

    Georgia Schäfer

    2015-05-01

    Full Text Available Currently, seven viruses, namely Epstein-Barr virus (EBV, Kaposi’s sarcoma-associated herpes virus (KSHV, high-risk human papillomaviruses (HPVs, Merkel cell polyomavirus (MCPyV, hepatitis B virus (HBV, hepatitis C virus (HCV and human T cell lymphotropic virus type 1 (HTLV-1, have been described to be consistently associated with different types of human cancer. These oncogenic viruses belong to distinct viral families, display diverse cell tropism and cause different malignancies. A key to their pathogenicity is attachment to the host cell and entry in order to replicate and complete their life cycle. Interaction with the host cell during viral entry is characterized by a sequence of events, involving viral envelope and/or capsid molecules as well as cellular entry factors that are critical in target cell recognition, thereby determining cell tropism. Most oncogenic viruses initially attach to cell surface heparan sulfate proteoglycans, followed by conformational change and transfer of the viral particle to secondary high-affinity cell- and virus-specific receptors. This review summarizes the current knowledge of the host cell surface factors and molecular mechanisms underlying oncogenic virus binding and uptake by their cognate host cell(s with the aim to provide a concise overview of potential target molecules for prevention and/or treatment of oncogenic virus infection.

  6. A New Acquisition Algorithm with Elimination Side Peak for All BOC Signals

    Directory of Open Access Journals (Sweden)

    Fang Liu

    2015-01-01

    Full Text Available A new inhibition side peak acquisition (ISPA algorithm is proposed for binary offset carrier (BOC modulated signals, which will be utilized in global navigation satellite systems (GNSS. We eliminate all side peaks of the BOC correlation function (CF by structuring special sequences composed of PRN code and cycle rectangular sequences. The new algorithm can be applied to both generic sine- and cosine-phased BOC signals, as well as to all modulation orders. Theoretical and simulation results demonstrate that the new algorithm can completely eliminate the ambiguity threat in the acquisition process, and it can adapt to lower SNR. In addition, this algorithm is better than the traditional algorithms in acquisition performance and inhibition side peak ability.

  7. Entry, Descent, and Landing Operations Analysis for the Stardust Entry Capsule

    Science.gov (United States)

    Desai, Prasun N.; Lyons, Dan T.; Tooley, Jeff; Kangas, Julie

    2008-01-01

    On the morning of January 15, 2006, the Stardust capsule successfully landed at the Utah Test and Training range in northwest Utah returning cometary samples from the comet Wild-2. An overview of the entry, descent, and landing (EDL) trajectory analysis that was performed for targeting during the mission operations phase upon final approach to Earth is described. The final orbit determination solution produced an inertial entry flight-path angle of -8.21 deg (the desired nominal value) with a 3-sigma uncertainty of +/-0.0017 deg (2% of the requirement). The navigation and EDL operations effort accurately delivered the entry capsule to the desired landing site. The final landing location was 8.1 km from the target, which was well within the allowable landing area. Overall, the Earth approach operation procedures worked well and there were no issues (logistically or performance based) that arose. As a result, the process of targeting a capsule from an interplanetary trajectory and accurately landing it on Earth was successfully demonstrated.

  8. Region 9 National Pollution Discharge Elimination System (NPDES) Facilities

    Data.gov (United States)

    U.S. Environmental Protection Agency — Point geospatial dataset representing locations of NPDES Facilities. NPDES (National Pollution Discharge Elimination System) is an EPA permit program that regulates...

  9. Analysis of polarization methods for elimination of power overshoot in microbial fuel cells

    KAUST Repository

    Watson, Valerie J.

    2011-01-01

    Polarization curves from microbial fuel cells (MFCs) often show an unexpectedly large drop in voltage with increased current densities, leading to a phenomenon in the power density curve referred to as "power overshoot". Linear sweep voltammetry (LSV, 1 mV s- 1) and variable external resistances (at fixed intervals of 20 min) over a single fed-batch cycle in an MFC both resulted in power overshoot in power density curves due to anode potentials. Increasing the anode enrichment time from 30 days to 100 days did not eliminate overshoot, suggesting that insufficient enrichment of the anode biofilm was not the primary cause. Running the reactor at a fixed resistance for a full fed-batch cycle (~ 1 to 2 days), however, completely eliminated the overshoot in the power density curve. These results show that long times at a fixed resistance are needed to stabilize current generation by bacteria in MFCs, and that even relatively slow LSV scan rates and long times between switching circuit loads during a fed-batch cycle may produce inaccurate polarization and power density results for these biological systems. © 2010 Elsevier B.V. All rights reserved.

  10. 19 CFR 145.24 - Amendment of entry.

    Science.gov (United States)

    2010-04-01

    ... director is satisfied that the objection is valid and timely, he shall amend the mail entry. If the duty... 19 Customs Duties 2 2010-04-01 2010-04-01 false Amendment of entry. 145.24 Section 145.24 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE...

  11. 19 CFR 4.35 - Unlading outside port of entry.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Unlading outside port of entry. 4.35 Section 4.35 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE... of entry. (a) Upon written application from the interested party, the port director concerned, if...

  12. Strategic advantage and the optimal exercise of entry options

    NARCIS (Netherlands)

    E.C. Perotti; S. Rossetto

    2001-01-01

    We investigate the timing and the valuation of strategic investment aimed at enhancing entry opportunities in related market segments. As demand is uncertain, entry options should be exercised at the optimal time, trading off the market share gain against the option to wait until more information is

  13. The Importance of Prior Probabilities for Entry Page Search

    NARCIS (Netherlands)

    Kraaij, W.; Westerveld, T.H.W.; Hiemstra, D.

    2002-01-01

    An important class of searches on the world-wide-web has the goal to find an entry page (homepage) of an organisation. Entry page search is quite different from Ad Hoc search. Indeed a plain Ad Hoc system performs disappointingly. We explored three non-content features of web pages: page length, num

  14. 18 CFR 33.5 - Proposed accounting entries.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Proposed accounting... § 33.5 Proposed accounting entries. If the applicant is required to maintain its books of account in... present proposed accounting entries showing the effect of the transaction with sufficient detail...

  15. 15 CFR 2011.204 - Entry of specialty sugars.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Entry of specialty sugars. 2011.204... UNITED STATES TRADE REPRESENTATIVE ALLOCATION OF TARIFF-RATE QUOTA ON IMPORTED SUGARS, SYRUPS AND MOLASSES Specialty Sugar § 2011.204 Entry of specialty sugars. An importer or the importer's agent...

  16. Strategic advantage and the optimal exercise of entry options

    NARCIS (Netherlands)

    Perotti, E.C.; Rossetto, S.

    2001-01-01

    We investigate the timing and the valuation of strategic investment aimed at enhancing entry opportunities in related market segments. As demand is uncertain, entry options should be exercised at the optimal time, trading off the market share gain against the option to wait until more information is

  17. Racial/Ethnic Disparities in ADHD Diagnosis by Kindergarten Entry

    Science.gov (United States)

    Morgan, Paul L.; Hillemeier, Marianne M.; Farkas, George; Maczuga, Steve

    2014-01-01

    Background: Whether and to what extent racial/ethnic disparities in attention-deficit/hyperactivity disorder (ADHD) diagnosis occur by kindergarten entry is currently unknown. We investigated risk factors associated with an ADHD diagnosis by kindergarten entry generally, and specifically whether racial/ethnic disparities in ADHD diagnosis occur by…

  18. Foreign Bank Entry and Credit Allocation in Emerging Markets

    NARCIS (Netherlands)

    Degryse, H.A.; Havrylchyk, O.; Jurzyk, E.; Kozak, S.

    2009-01-01

    We employ a unique data set containing bank-specific information to explore how foreign bank entry determines credit allocation in emerging markets. We investigate the impact of the mode of foreign entry (greenfield or takeover) on banks’ portfolio allocation to borrowers with different degrees of

  19. Foreign Bank Entry and Credit Allocation in Emerging Markets

    NARCIS (Netherlands)

    Degryse, H.A.; Havrylchyk, O.; Jurzyk, E.; Kozak, S.

    2009-01-01

    We employ a unique data set containing bank-specific information to explore how foreign bank entry determines credit allocation in emerging markets. We investigate the impact of the mode of foreign entry (greenfield or takeover) on banks’ portfolio allocation to borrowers with different degrees of i

  20. Racial/Ethnic Disparities in ADHD Diagnosis by Kindergarten Entry

    Science.gov (United States)

    Morgan, Paul L.; Hillemeier, Marianne M.; Farkas, George; Maczuga, Steve

    2014-01-01

    Background: Whether and to what extent racial/ethnic disparities in attention-deficit/hyperactivity disorder (ADHD) diagnosis occur by kindergarten entry is currently unknown. We investigated risk factors associated with an ADHD diagnosis by kindergarten entry generally, and specifically whether racial/ethnic disparities in ADHD diagnosis occur by…

  1. 19 CFR 146.64 - Entry for warehouse.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry for warehouse. 146.64 Section 146.64 Customs... (CONTINUED) FOREIGN TRADE ZONES Transfer of Merchandise From a Zone § 146.64 Entry for warehouse. (a) Foreign... status may not be entered for warehouse from a zone. Merchandise in nonprivileged foreign...

  2. 19 CFR 151.63 - Information on entry summary.

    Science.gov (United States)

    2010-04-01

    ... estimated or actual net weight of the wool or hair in its condition as imported, its total estimated clean... THE TREASURY (CONTINUED) EXAMINATION, SAMPLING, AND TESTING OF MERCHANDISE Wool and Hair § 151.63 Information on entry summary. Each entry summary covering wool or hair subject to duty at a rate per...

  3. 31 CFR 357.0 - Book-entry systems.

    Science.gov (United States)

    2010-07-01

    ... non-Internet-based book-entry system maintained by Treasury for purchasing and holding marketable...) TreasuryDirect ®. TreasuryDirect is a book-entry, online system maintained by the Department of the Treasury for purchasing and holding eligible marketable Treasury securities, United States Savings...

  4. 19 CFR 146.63 - Entry for consumption.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry for consumption. 146.63 Section 146.63... TREASURY (CONTINUED) FOREIGN TRADE ZONES Transfer of Merchandise From a Zone § 146.63 Entry for consumption... status may be entered for consumption from a zone. (b) Zone-restricted merchandise. Merchandise in a zone...

  5. Extended High-Gain Observer for Mars Entry Guidance

    Directory of Open Access Journals (Sweden)

    Pingyuan Cui

    2013-02-01

    Full Text Available To deliver a Mars entry vehicle to the prescribed parachute deployment point, active entry guidance is essential. This paper addresses the problem of Mars atmospheric entry guidance through drag tracking method with extended high gain observer. First, an extended high gain observer combined with feedback linearization is applied in drag tracking for Mars entry longitudinal guidance.  The observer estimates the drag and drag rate for drag tracking, estimates the perturbation due to model uncertainty and disturbance, and compensate for the perturbation by canceling its estimate. Then, bank reversal is adopted in the lateral plane to reduce the cross-range error. Finally, Mars entry simulation is performed to assess the performance of the adaptive guidance law. The results demonstrate that the proposed guidance law exhibits good performance.

  6. Strategic Orientation and Order of Market Entry of Food Firms

    Directory of Open Access Journals (Sweden)

    rasoul ghollamzadeh

    2011-12-01

    One of the strategic launch decisions is the order of market entry. Adoption of a suitable competitive strategy is dependent on firm's decision on market entry earlier or later than competitors. This research aims to analyze the relationship between order of market entry and firms’ strategic orientation. For this purpose, three strategies have been defined as cost leadership strategy, innovation differentiation strategy and marketing differentiation strategy. In this study, the essential question is whether firms use a different strategy based on their order of market entry? The proposed model has been examined on a sample of 102 manufacturing companies in the food industry using Structural Equation Modeling based on the methodology of Partial Least Squares (PLS. Findings indicate a direct influence of order of market entry on adopting a particular strategy by the firms, so that pioneer companies tend more to use differentiation strategy at two levels of marketing and innovation, while cost leadership is more common among followers.

  7. Strategic Orientation and Order of Market Entry of Food Firms

    Directory of Open Access Journals (Sweden)

    Rasoul Ghollamzadeh

    2011-01-01

    Full Text Available One of the strategic launch decisions is the order of market entry. Adoption of a suitable competitive strategy is dependent on firm's decision on market entry earlier or later than competitors. This research aims to analyze the relationship between order of market entry and firms’ strategic orientation. For this purpose, three strategies have been defined as cost leadership strategy, innovation differentiation strategy and marketing differentiation strategy. In this study, the essential question is whether firms use a different strategy based on their order of market entry? The proposed model has been examined on a sample of 102 manufacturing companies in the food industry using Structural Equation Modeling based on the methodology of Partial Least Squares (PLS. Findings indicate a direct influence of order of market entry on adopting a particular strategy by the firms, so that pioneer companies tend more to use differentiation strategy at two levels of marketing and innovation, while cost leadership is more common among followers.

  8. Market entry decisions: effects of absolute and relative confidence.

    Science.gov (United States)

    Bolger, Fergus; Pulford, Briony D; Colman, Andrew M

    2008-01-01

    In a market entry game, the number of entrants usually approaches game-theoretic equilibrium quickly, but in real-world markets business start-ups typically exceed market capacity, resulting in chronically high failure rates and suboptimal industry profits. Excessive entry has been attributed to overconfidence arising when expected payoffs depend partly on skill. In an experimental test of this hypothesis, 96 participants played 24 rounds of a market entry game, with expected payoffs dependent partly on skill on half the rounds, after their confidence was manipulated and measured. The results provide direct support for the hypothesis that high levels of confidence are largely responsible for excessive entry, and they suggest that absolute confidence, independent of interpersonal comparison, rather than confidence about one's abilities relative to others, drives excessive entry decisions when skill is involved.

  9. Cell cycle arrest by prostaglandin A1 at the G1/S phase interface with up-regulation of oncogenes in S-49 cyc- cells

    Science.gov (United States)

    Hughes-Fulford, M.

    1994-01-01

    Our previous studies have implied that prostaglandins inhibit cell growth independent of cAMP. Recent reports, however, have suggested that prostaglandin arrest of the cell cycle may be mediated through protein kinase A. In this report, in order to eliminate the role of c-AMP in prostaglandin mediated cell cycle arrest, we use the -49 lymphoma variant (cyc-) cells that lack adenylate cyclase activity. We demonstrate that dimethyl prostaglandin A1 (dmPGA1) inhibits DNA synthesis and cell growth in cyc- cells. DNA synthesis is inhibited 42% by dmPGA1 (50 microM) despite the fact that this cell line lacks cellular components needed for cAMP generation. The ability to decrease DNA synthesis depends upon the specific prostaglandin structure with the most effective form possessing the alpha, beta unsaturated ketone ring. Dimethyl PGA1 is most effective in inhibiting DNA synthesis in cyc- cells, with prostaglandins PGE1 and PGB1 being less potent inhibitors of DNA synthesis. DmPGE2 caused a significant stimulation of DNA synthesis. S-49 cyc- variant cells exposed to (30-50 microns) dmPGA1, arrested in the G1 phase of the cell cycle within 24 h. This growth arrest was reversed when the prostaglandin was removed from the cultured cells; growth resumed within hours showing that this treatment is not toxic. The S-49 cyc- cells were chosen not only for their lack of adenylate cyclase activity, but also because their cell cycle has been extensively studied and time requirements for G1, S, G2, and M phases are known. Within hours after prostaglandin removal the cells resume active DNA synthesis, and cell number doubles within 15 h suggesting rapid entry into S-phase DNA synthesis from the G1 cell cycle block.(ABSTRACT TRUNCATED AT 250 WORDS).

  10. Internal ribosome entry site mediates protein synthesis in yeast Pichia pastoris.

    Science.gov (United States)

    Liang, Shuli; Lin, Ying; Li, Cheng; Ye, Yanrui

    2012-05-01

    The imitation of translation, as mediated by internal ribosome entry sites, has not yet been reported in Pichia pastoris. An IRES element from Saccharomyces cerevisiae was demonstrated to direct the translation of a dicistronic mRNA in P. pastoris. The 5′-untranslated region of GPR1 mRNA, termed GPR, was cloned into a dual reporter construct containing an upstream Rhizomucor miehei lipase (RML) and a downstream β-galactosidase gene (lacZ) from Escherichia coli BL21. After being transformed into P. pastoris, the RML gene and lacZ were simultaneously expressed. The possibility of DNA rearrangement, spurious splicing, or cryptic promoter in the GPR sequence were eliminated, indicating that expression of a second ORF was IRES-dependent. These findings strongly suggested that the IRES-dependent translation initiation mechanism is conserved in P. pastoris and provides a useful means to express multiple genes simultaneously.

  11. 9 CFR 381.208 - Poultry products offered for entry and entered to be handled and transported as domestic; entry...

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Poultry products offered for entry and entered to be handled and transported as domestic; entry into official establishments; transportation. 381.208 Section 381.208 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY ORGANIZATION AND...

  12. Clathrin- and caveolin-independent entry of human papillomavirus type 16--involvement of tetraspanin-enriched microdomains (TEMs.

    Directory of Open Access Journals (Sweden)

    Gilles Spoden

    Full Text Available BACKGROUND: Infectious entry of human papillomaviruses into their host cells is an important step in the viral life cycle. For cell binding these viruses use proteoglycans as initial attachment sites. Subsequent transfer to a secondary receptor molecule seems to be involved in virus uptake. Depending on the papillomavirus subtype, it has been reported that entry occurs by clathrin- or caveolin-mediated mechanisms. Regarding human papillomavirus type 16 (HPV16, the primary etiologic agent for development of cervical cancer, clathrin-mediated endocytosis was described as infectious entry pathway. METHODOLOGY/PRINCIPAL FINDINGS: Using immunofluorescence and infection studies we show in contrast to published data that infectious entry of HPV16 occurs in a clathrin- and caveolin-independent manner. Inhibition of clathrin- and caveolin/raft-dependent endocytic pathways by dominant-negative mutants and siRNA-mediated knockdown, as well as inhibition of dynamin function, did not impair infection. Rather, we provide evidence for involvement of tetraspanin-enriched microdomains (TEMs in HPV16 endocytosis. Following cell attachment, HPV16 particles colocalized with the tetraspanins CD63 and CD151 on the cell surface. Notably, tetraspanin-specific antibodies and siRNA inhibited HPV16 cell entry and infection, confirming the importance of TEMs for infectious endocytosis of HPV16. CONCLUSIONS/SIGNIFICANCE: Tetraspanins fulfill various roles in the life cycle of a number of important viral pathogens, including human immunodeficiency virus (HIV and hepatitis C virus (HCV. However, their involvement in endocytosis of viral particles has not been proven. Our data indicate TEMs as a novel clathrin- and caveolin-independent invasion route for viral pathogens and especially HPV16.

  13. Changes in Cerebral Hemodynamics during Complex Motor Learning by Character Entry into Touch-Screen Terminals.

    Directory of Open Access Journals (Sweden)

    Akira Sagari

    Full Text Available Studies of cerebral hemodynamics during motor learning have mostly focused on neurorehabilitation interventions and their effectiveness. However, only a few imaging studies of motor learning and the underlying complex cognitive processes have been performed.We measured cerebral hemodynamics using near-infrared spectroscopy (NIRS in relation to acquisition patterns of motor skills in healthy subjects using character entry into a touch-screen terminal. Twenty healthy, right-handed subjects who had no previous experience with character entry using a touch-screen terminal participated in this study. They were asked to enter the characters of a randomly formed Japanese syllabary into the touch-screen terminal. All subjects performed the task with their right thumb for 15 s alternating with 25 s of rest for 30 repetitions. Performance was calculated by subtracting the number of incorrect answers from the number of correct answers, and gains in motor skills were evaluated according to the changes in performance across cycles. Behavioral and oxygenated hemoglobin concentration changes across task cycles were analyzed using Spearman's rank correlations.Performance correlated positively with task cycle, thus confirming motor learning. Hemodynamic activation over the left sensorimotor cortex (SMC showed a positive correlation with task cycle, whereas activations over the right prefrontal cortex (PFC and supplementary motor area (SMA showed negative correlations.We suggest that increases in finger momentum with motor learning are reflected in the activity of the left SMC. We further speculate that the right PFC and SMA were activated during the early phases of motor learning, and that this activity was attenuated with learning progress.

  14. Bipolar mood cycles and lunar tidal cycles.

    Science.gov (United States)

    Wehr, T A

    2017-01-24

    In 17 patients with rapid cycling bipolar disorder, time-series analyses detected synchronies between mood cycles and three lunar cycles that modulate the amplitude of the moon's semi-diurnal gravimetric tides: the 14.8-day spring-neap cycle, the 13.7-day declination cycle and the 206-day cycle of perigee-syzygies ('supermoons'). The analyses also revealed shifts among 1:2, 1:3, 2:3 and other modes of coupling of mood cycles to the two bi-weekly lunar cycles. These shifts appear to be responses to the conflicting demands of the mood cycles' being entrained simultaneously to two different bi-weekly lunar cycles with slightly different periods. Measurements of circadian rhythms in body temperature suggest a biological mechanism through which transits of one of the moon's semi-diurnal gravimetric tides might have driven the patients' bipolar cycles, by periodically entraining the circadian pacemaker to its 24.84-h rhythm and altering the pacemaker's phase-relationship to sleep in a manner that is known to cause switches from depression to mania.Molecular Psychiatry advance online publication, 24 January 2017; doi:10.1038/mp.2016.263.

  15. Firm entry and aggregate efficiency growth: An optimal dynamic - Program of entry and R&D investment

    Directory of Open Access Journals (Sweden)

    Asma Raies

    2013-12-01

    Full Text Available The effect of entry on the aggregate efficiency growth is still theoretically and empirically unresolved. Many studies focused on this effect in short and long-run, without considering the dynamic transition and how do entry affect the convergence of the industrytoward its long-run equilibrium? This paper aims to provide an answer and to fill this gap by employingoptimal control principles. Our model exhibits saddlepath stability and shows that the effect of entry and entry liberalizing policy (reducing the entry cost on the aggregate efficiency growth may be positive, negative or nil depending on the industry’s initial characteristics (size and R&D. This theoretical result can justify the inconclusive current empirical evidence.

  16. Novel Ganglioside-mediated Entry of Botulinum Neurotoxin Serotype D into Neurons

    Energy Technology Data Exchange (ETDEWEB)

    Kroken, Abby R.; Karalewitz, Andrew P.-A.; Fu, Zhuji; Kim, Jung-Ja P.; Barbieri, Joseph T. (MCW)

    2012-02-07

    Botulinum Neurotoxins (BoNTs) are organized into seven serotypes, A-G. Although several BoNT serotypes enter neurons through synaptic vesicle cycling utilizing dual receptors (a ganglioside and a synaptic vesicle-associated protein), the entry pathway of BoNT/D is less well understood. Although BoNT/D entry is ganglioside-dependent, alignment and structural studies show that BoNT/D lacks key residues within a conserved ganglioside binding pocket that are present in BoNT serotypes A, B, E, F, and G, which indicate that BoNT/D-ganglioside interactions may be unique. In this study BoNT/D is shown to have a unique association with ganglioside relative to the other BoNT serotypes, utilizing a ganglioside binding loop (GBL, residues Tyr-1235-Ala-1245) within the receptor binding domain of BoNT/D (HCR/D) via b-series gangliosides, including GT1b, GD1b, and GD2. HCR/D bound gangliosides and entered neurons dependent upon the aromatic ring of Phe-1240 within the GBL. This is the first BoNT-ganglioside interaction that is mediated by a phenylalanine. In contrast, Trp-1238, located near the N terminus of the ganglioside binding loop, was mostly solvent-inaccessible and appeared to contribute to maintaining the loop structure. BoNT/D entry and intoxication were enhanced by membrane depolarization via synaptic vesicle cycling, where HCR/D colocalized with synaptophysin, a synaptic vesicle marker, but immunoprecipitation experiments did not detect direct association with synaptic vesicle protein 2. Thus, BoNT/D utilizes unique associations with gangliosides and synaptic vesicles to enter neurons, which may facilitate new neurotoxin therapies.

  17. Gains and Pitfalls of Quantifier Elimination as a Teaching Tool

    Science.gov (United States)

    Oldenburg, Reinhard

    2015-01-01

    Quantifier Elimination is a procedure that allows simplification of logical formulas that contain quantifiers. Many mathematical concepts are defined in terms of quantifiers and especially in calculus their use has been identified as an obstacle in the learning process. The automatic deduction provided by quantifier elimination thus allows…

  18. Deferring Elimination of Design Alternatives in Object Oriented Methods

    NARCIS (Netherlands)

    Aksit, Mehmet; Marcelloni, Francesco

    2001-01-01

    While developing systems, software engineers generally have to deal with a large number of design alternatives. Current object-oriented methods aim to eliminate design alternatives whenever they are generated. Alternatives, however, should be eliminated only when sufficient information to take such

  19. Competition: Butterflies eliminate milkweed bugs from a Caribbean Island.

    Science.gov (United States)

    Blakley, Nigel R; Dingle, Hugh

    1978-01-01

    By eliminating the food plant, Asclepias curassavica, monarch butterflies, Danaus plexippus, have virtually eliminated milkweed bugs, Oncopeltus spp., from the island of Barbados. The relatively open terrain of Barbados means the plants have no refuge; the butterflies survive on an alternate milkweed food plant, Calotropis procera, whose thick-walled pods make seeds unavailable to the bugs.

  20. Research on Eliminating Set-marks by Image Processing

    Institute of Scientific and Technical Information of China (English)

    SUN Zhi-hong; ZENG Yong-qi; LI Wei; CHEN Ming

    2005-01-01

    The causes of set-marks on woven fabrics were analyzed and the commonly used methods to eliminate this kind of defect were introduced. A non-contact method based on image processing technique to detect the cloth-fell displacement and eliminate set-marks was described in detail in this paper.

  1. On the Importance of Elimination Heuristics in Lazy Propagation

    DEFF Research Database (Denmark)

    Madsen, Anders Læsø; Butz, Cory J.

    2012-01-01

    Belief update in a Bayesian network using Lazy Propagation (LP) proceeds by message passing over a junction tree (JT). In the process of computing a message, a set of variables is eliminated. As the JT provides only a partial order on the elimination of variables, it is necessary to identify elim...

  2. 77 FR 30871 - Implementing the Prison Rape Elimination Act

    Science.gov (United States)

    2012-05-23

    ... May 23, 2012 Part VI The President Memorandum of May 17, 2012--Implementing the Prison Rape... 3-- #0;The President ] Memorandum of May 17, 2012 Implementing the Prison Rape Elimination Act... assault on human dignity and an affront to American values. The Prison Rape Elimination Act of 2003...

  3. Eliminating Community Services--The Leisure Services Example.

    Science.gov (United States)

    Crompton, John L.; Lamb, Charles W.

    1980-01-01

    Illustrates the importance of adopting a formal strategy for eliminating programs. Outlines a three-step procedure for identifying candidates for elimination, evaluating these programs, and implementing the decision. The procedure provides community service agencies with guidelines for better serving the needs of their clientele. (SK)

  4. Kinases required in hepatitis C virus entry and replication highlighted by small interference RNA screening.

    Science.gov (United States)

    Trotard, Maud; Lepère-Douard, Charlotte; Régeard, Morgane; Piquet-Pellorce, Claire; Lavillette, Dimitri; Cosset, François-Loic; Gripon, Philippe; Le Seyec, Jacques

    2009-11-01

    The entry pathway of the hepatitis C virus (HCV), a major human pathogen, into the cell is incompletely defined. To better characterize this viral life cycle stage, we screened a small interfering RNA library dedicated to the membrane trafficking and remodeling with the infection model of Huh-7.5.1 cells by HCV pseudoparticles (HCVpp). Results showed that the down-regulation of different factors implied in clathrin-mediated endocytosis (CME) inhibits HCVpp cell infection. In addition, knockdown of the phosphatidylinositol 4-kinase type III-alpha (PI4KIIIalpha) prevented infection by HCVpp or by cell-culture grown JFH-1-based HCV. Moreover, the replication activity of an HCV replicon was also affected by the PI4KIIIalpha knockdown. Additional investigations on the different members of the PI4K family revealed that the presence of PI4KIIIbeta in the host cells influenced their susceptibility to HCVpp infection and their capacity to sustain the HCV replication. The PI4KIII involvement during the HCV life cycle seemed to occur by other ways than the control of the CME or of the membranous expression of HCV receptors. Finally, our library screening completed data on the CME-dependant entry route of HCV and identified 2 kinases, PI4KIIIalpha and beta, as relevant potential therapeutic targets.

  5. X-33 Attitude Control System Design for Ascent, Transition, and Entry Flight Regimes

    Science.gov (United States)

    Hall, Charles E.; Gallaher, Michael W.; Hendrix, Neal D.

    1998-01-01

    The Vehicle Control Systems Team at Marshall Space Flight Center, Systems Dynamics Laboratory, Guidance and Control Systems Division is designing under a cooperative agreement with Lockheed Martin Skunkworks, the Ascent, Transition, and Entry flight attitude control system for the X-33 experimental vehicle. Ascent flight control begins at liftoff and ends at linear aerospike main engine cutoff (NECO) while Transition and Entry flight control begins at MECO and concludes at the terminal area energy management (TAEM) interface. TAEM occurs at approximately Mach 3.0. This task includes not only the design of the vehicle attitude control systems but also the development of requirements for attitude control system components and subsystems. The X-33 attitude control system design is challenged by a short design cycle, the design environment (Mach 0 to about Mach 15), and the X-33 incremental test philosophy. The X-33 design-to-launch cycle of less than 3 years requires a concurrent design approach while the test philosophy requires design adaptation to vehicle variations that are a function of Mach number and mission profile. The flight attitude control system must deal with the mixing of aerosurfaces, reaction control thrusters, and linear aerospike engine control effectors and handle parasitic effects such as vehicle flexibility and propellant sloshing from the uniquely shaped propellant tanks. The attitude control system design is, as usual, closely linked to many other subsystems and must deal with constraints and requirements from these subsystems.

  6. Swiss Army Pathogen: The Salmonella Entry Toolkit

    Directory of Open Access Journals (Sweden)

    Peter J. Hume

    2017-08-01

    Full Text Available Salmonella causes disease in humans and animals ranging from mild self-limiting gastroenteritis to potentially life-threatening typhoid fever. Salmonellosis remains a considerable cause of morbidity and mortality globally, and hence imposes a huge socio-economic burden worldwide. A key property of all pathogenic Salmonella strains is the ability to invade non-phagocytic host cells. The major determinant of this invasiveness is a Type 3 Secretion System (T3SS, a molecular syringe that injects virulence effector proteins directly into target host cells. These effectors cooperatively manipulate multiple host cell signaling pathways to drive pathogen internalization. Salmonella does not only rely on these injected effectors, but also uses several other T3SS-independent mechanisms to gain entry into host cells. This review summarizes our current understanding of the methods used by Salmonella for cell invasion, with a focus on the host signaling networks that must be coordinately exploited for the pathogen to achieve its goal.

  7. On Heatshield Shapes for Mars Entry Capsules

    Science.gov (United States)

    Prabhu, DInesh K.; Saunders, David A.

    2012-01-01

    The 70deg sphere-cone - the standard geometry for all US Mars entry missions - is thoroughly examined via flow field simulations at a select few peak heating points along candidate flight trajectories. Emphasis is placed on turbulent heating based on the Baldwin- Lomax turbulence model. It is shown that increased leeward turbulent heating for a 70 sphere-cone flying at angle of attack is primarily due to the discontinuity in curvature between the spherical nose cap and the conical frustum - the attachment of the sonic line at this sphere-cone junction leads to a supersonic edge Mach number over the leeward acreage. In an attempt to mitigate this problem of elevated turbulent heating, alternate geometries, without any curvature discontinuities in the acreage, are developed. Two approaches, one based on nonlinear optimization with constraints, and one based on the use of non-uniform rational B-splines, are considered. All configurations examined remain axisymmetric. The aerothermal performance of alternate geometries is shown to be superior to that of the 70 sphere-cone.

  8. Inflatable Emergency Atmospheric-Entry Vehicles

    Science.gov (United States)

    Jones, Jack; Hall, Jeffrey; Wu, Jiunn Jeng

    2004-01-01

    In response to the loss of seven astronauts in the Space Shuttle Columbia disaster, large, lightweight, inflatable atmospheric- entry vehicles have been proposed as means of emergency descent and landing for persons who must abandon a spacecraft that is about to reenter the atmosphere and has been determined to be unable to land safely. Such a vehicle would act as an atmospheric decelerator at supersonic speed in the upper atmosphere, and a smaller, central astronaut pod could then separate at lower altitudes and parachute separately to Earth. Astronaut-rescue systems that have been considered previously have been massive, and the cost of designing them has exceeded the cost of fabrication of a space shuttle. In contrast, an inflatable emergency-landing vehicle according to the proposal would have a mass between 100 and 200 kg, could be stored in a volume of approximately 0.2 to 0.4 cu m, and could likely be designed and built much less expensively. When fully inflated, the escape vehicle behaves as a large balloon parachute, or ballute. Due to very low mass-per-surface area, a large radius, and a large coefficient of drag, ballutes decelerate at much higher altitudes and with much lower heating rates than the space shuttle. Although the space shuttle atmospheric reentry results in surface temperatures of about 1,600 C, ballutes can be designed for maximum temperatures below 600 C. This allows ballutes to be fabricated with lightweight ZYLON(Registered TradeMark) or polybenzoxazole (PBO), or equivalent.

  9. Intracellular route of canine parvovirus entry.

    Science.gov (United States)

    Vihinen-Ranta, M; Kalela, A; Mäkinen, P; Kakkola, L; Marjomäki, V; Vuento, M

    1998-01-01

    The present study was designed to investigate the endocytic pathway involved in canine parvovirus (CPV) infection. Reduced temperature (18 degrees C) or the microtubule-depolymerizing drug nocodazole was found to inhibit productive infection of canine A72 cells by CPV and caused CPV to be retained in cytoplasmic vesicles as indicated by immunofluorescence microscopy. Consistent with previously published results, these data indicate that CPV enters a host cell via an endocytic route and further suggest that microtubule-dependent delivery of CPV to late endosomes is required for productive infection. Cytoplasmic microinjection of CPV particles was used to circumvent the endocytosis and membrane fusion steps in the entry process. Microinjection experiments showed that CPV particles which were injected directly into the cytoplasm, thus avoiding the endocytic pathway, were unable to initiate progeny virus production. CPV treated at pH 5.0 prior to microinjection was unable to initiate virus production, showing that factors of the endocytic route other than low pH are necessary for the initiation of infection by CPV.

  10. HTCC: Broad Range Inhibitor of Coronavirus Entry.

    Directory of Open Access Journals (Sweden)

    Aleksandra Milewska

    Full Text Available To date, six human coronaviruses have been known, all of which are associated with respiratory infections in humans. With the exception of the highly pathogenic SARS and MERS coronaviruses, human coronaviruses (HCoV-NL63, HCoV-OC43, HCoV-229E, and HCoV-HKU1 circulate worldwide and typically cause the common cold. In most cases, infection with these viruses does not lead to severe disease, although acute infections in infants, the elderly, and immunocompromised patients may progress to severe disease requiring hospitalization. Importantly, no drugs against human coronaviruses exist, and only supportive therapy is available. Previously, we proposed the cationically modified chitosan, N-(2-hydroxypropyl-3-trimethylammonium chitosan chloride (HTCC, and its hydrophobically-modified derivative (HM-HTCC as potent inhibitors of the coronavirus HCoV-NL63. Here, we show that HTCC inhibits interaction of a virus with its receptor and thus blocks the entry. Further, we demonstrate that HTCC polymers with different degrees of substitution act as effective inhibitors of all low-pathogenic human coronaviruses.

  11. Lysosomotropic agents as HCV entry inhibitors

    Directory of Open Access Journals (Sweden)

    Nawaz Zafar

    2011-04-01

    Full Text Available Abstract HCV has two envelop proteins named as E1 and E2 which play an important role in cell entry through two main pathways: direct fusion at the plasma membrane and receptor-mediated endocytosis. Fusion of the HCV envelope proteins is triggered by low pH within the endosome. Lysosomotropic agents (LA such as Chloroquine and Ammonium chloride (NH4Cl are the weak bases and penetrate in lysosome as protonated form and increase the intracellular pH. To investigate the antiviral effect of LA (Chloroquine and NH4Cl on pH dependent endocytosis, HCV pseudoparticles (HCVpp of 1a and 3a genotype were produced and used to infect liver cells. The toxicological effects of Chloroquine and NH4Cl were tested in liver cells through MTT cell proliferation assay. For antiviral screening of Chloroquine and NH4Cl, liver cells were infected with HCVpp of 3a and 1a genotype in the presence or absence of different concentrations of Chloroquine and NH4Cl and there luciferase activity was determined by using a luminometer. The results demonstrated that Chloroquine and NH4Cl showed more than 50% reduction of virus infectivity at 50 μM and 10 mM concentrations respectively. These results suggest that inhibition of HCV at fusion step by increasing the lysosomal pH will be better option to treat chronic HCV.

  12. Repression of c-Myc responsive genes in cycling cells causes G1 arrest through reduction of cyclin E/CDK2 kinase activity

    NARCIS (Netherlands)

    Berns, K.; Hijmans, E.M.; Bernards, R.A.

    1997-01-01

    The c-myc gene encodes a sequence-specific DNA binding protein involved in proliferation and oncogenesis. Activation of c-myc expression in quiescent cells is sufficient to mediate cell cycle entry, whereas inhibition of c-myc expression causes cycling cells to withdraw from the cell cycle. To searc

  13. Theoretical Study on CO2 Transcritical Cycle Combined Ejector Cycle Refrigeration System

    Institute of Scientific and Technical Information of China (English)

    卢苇; 马一太; 李敏霞; 查世彤

    2003-01-01

    Chlorofluorocarbons(CFCs) or hydrochlorofluorocarbons(HCFCs) are as main refrigerants used in traditional refrigeration systems driven by electricity from burning fossil fuels, which is regarded as one of the major reasons for ozone depletion (man-made refrigerants emission) and global warming (CO2 emission). So people pay more and more attention to natural refrigerants and energy saving technologies. An innovative system combining CO2 transcritical cycle with ejector cycle is proposed in this paper. The CO2 compression sub-cycle is powered by electricity with the characteristics of relatively high temperature in the gas cooler (defined as an intercooler by the proposed system). In order to recover the waste heat, an ejector sub-cycle operating with the natural refrigerants (NH3, H2O) is employed. The two sub-cycles are connected by an intercooler. This combined cycle joins the advantages of the two cycles together and eliminates the disadvantages. The influences of the evaporation temperature in CO2 compression sub-cycle, the evaporation temperature in the ejector sub-cycle, the temperature in the intercooler and the condensation temperature in the proposed system performance are discussed theoretically in this study. In addition, some unique features of the system are presented.

  14. Investment case concepts in leprosy elimination: A systematic review.

    Science.gov (United States)

    Tiwari, Anuj; Richardus, Jan Hendrik

    2016-03-01

    Leprosy continues to be a global public health problem, but draws less attention because 'prevalence based elimination' has been misinterpreted as eradication. The ongoing transmission of M. leprae has renewed interest in complete elimination. The aim of our study is to review systematically the literature regarding the elimination of leprosy, and to assess this information on its applicability for defining a Leprosy Elimination Investment Case (LEIC) based on Eradication Investment Case guidelines. A literature search was conducted using the MeSH subheadings and synonyms of leprosy. A total of 1007 articles were considered and 112 were included in the final selection. The search focused on the literature covering leprosy elimination and its public health aspects. The LEIC framework was adapted from an existing "Guide to Preparing an Eradication Investment Case". The LEIC framework provided 11 topics under which information was synthesized from the literature. The fields were categorised under sections: 1) Proposed investment; 2) Rationale for investing; 3) Issues to consider when moving from control to eradication; 4) Management and governance. Scanty quantitative data are available for developing a LEIC, particularly regarding disease burden, and new interventions that could contribute to elimination are not yet applied routinely. For monitoring global elimination, it is necessary to measure disease burden comprehensively, and contact centered preventive interventions should be part of a global elimination strategy. The biological and technical feasibility of elimination is not certain and advanced microbiological and operational research is necessary to understand transmission better. The current WHO road map for leprosy elimination is too vague and needs further structuring through a thoroughly prepared LEIC.

  15. Three-dimensional acceleration planning for atmospheric entry

    Science.gov (United States)

    Chen, David Teh-Han

    The next generation of reusable launch vehicles will benefit from an improved entry guidance algorithm. Improvements have been made to the current Space Shuttle entry guidance algorithm that will provide an ability to handle aborts, reach large crossranges, and provide complete onboard planning capability. Building on the entry guidance algorithm for the Space Shuttle, three versions of a three-dimensional acceleration based entry guidance algorithm have been created and tested. The Space Shuttle entry guidance algorithm is extended to three dimensions by planning the drag profile and the occurrence of bank reversals. The three versions of the planning algorithm that have been developed are a single bank reversal planner, a two bank reversal planner, and a single bank reversal update planner. Tests of the single and two bank reversal versions show that the planning algorithms are capable of producing feasible trajectories for a wide range of various entry conditions. Integration and testing of the update planning algorithm with a feedback linearizing control law in a high fidelity simulation developed by NASA Marshall has demonstrated the algorithm's ability to handle a variety of entry conditions in an onboard environment.

  16. A Further Look at X-33 Entry Guidance and Beyond

    Science.gov (United States)

    Lu, Ping

    1999-01-01

    An entry guidance design developed at Iowa State University (ISU) for the X-33 advanced technology demonstrator is outlined and compared with the X-33 entry guidance algorithms developed at NASA Marshall. Both designs are based on the Space Shuttle entry guidance concept, but significant improvements have been made to enhance the performance and reduce the complexity. The ISU design was incorporated into MAVERIC, a high fidelity vehicle simulation software for the X-33, and evaluated in Monte Carlo simulations against random dispersions in propulsion system, wind and atmospheric properties, aerodynamic coefficients, interaction between propulsion and aerodynamics, and navigation data. The simulations clearly demonstrated the capability and precision of the ISU entry guidance design in successfully guiding the X-33 in some rather difficult flight scenarios. As the entry guidance development for the X-33 is completing, this report also offers some review of the strength and limitations of the current Shuttle-based entry guidance framework, and finally some potential candidates for next generation of more capable and cost-effective entry guidance designs are discussed.

  17. The effects of foreign banks entry in emerging market economies

    Directory of Open Access Journals (Sweden)

    MSc. Florida Veljanoska

    2011-12-01

    Full Text Available This paper investigates the effects of foreign bank entry in emerging markets. We developed a picture of a multinational bank in an emerging markets by combining statistics from several sources, in order to explore broad range of effects that brings foreign bank entry in the developing countries. Some impacts of foreign bank entry have been thoroughly studied, while others are hardly mention. Entry of foreign bank brings large benefits to host country’s financial system and economies at large. This paper is studying those benefits very carefully, by analyzing the impact of foreign bank entry on economy, government, monetary policy, large enterprises, small and medium size enterprises, domestic bank etc. But, we also consider the fact that at the same time, foreign investment in the financial sector, rises some concerns, and therefore we analyze the negative effects as well. At the end we must admit that although there are some negative consequences from foreign bank entry in emerging markets, the benefits that arise from foreign banks penetration are much more, and this trend of foreign bank entry has brought new positive economic impulse in developing world.

  18. Analysis of limited-entry well tests in layered reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Hayes, M.R.; Naismith, J.D.A.; Gunasekera, D.L.; Fitzpatrick, A.J.

    1994-12-31

    Vertical permeability, often a key parameter in reservoir engineering, can be calculated from the analysis of a limited entry pressure transient. Analytical solutions for limited entry welltests, where a fraction of the reservoir interval is perforated, have been available in the literature for some time. This paper considers the evaluation of limited entry welltests in heterogeneous layered formations. A number of field examples have been analyzed using both conventional analytical techniques and a new general purpose numerical welltest analysis application. The examples show that in layered formations vertical permeability can be significantly under estimated by conventional analytical analysis.

  19. Predicting academic outcomes in an Australian graduate entry medical programme.

    Science.gov (United States)

    Puddey, Ian B; Mercer, Annette

    2014-02-15

    Predictive validity studies for selection criteria into graduate entry courses in Australia have been inconsistent in their outcomes. One of the reasons for this inconsistency may have been failure to have adequately considered background disciplines of the graduates as well as other potential confounding socio-demographic variables that may influence academic performance. Graduate entrants into the MBBS at The University of Western Australia between 2005 and 2012 were studied (N = 421). They undertook a 6-month bridging course, before joining the undergraduate-entry students for Years 3 through 6 of the medical course. Students were selected using their undergraduate Grade Point Average (GPA), Graduate Australian Medical School Admissions Test scores (GAMSAT) and a score from a standardised interview. Students could apply from any background discipline and could also be selected through an alternative rural entry pathway again utilising these 3 entry scores. Entry scores, together with age, gender, discipline background, rural entry status and a socioeconomic indicator were entered into linear regression models to determine the relative influence of each predictor on subsequent academic performance in the course. Background discipline, age, gender and selection through the rural pathway were variously related to each of the 3 entry criteria. Their subsequent inclusion in linear regression models identified GPA at entry, being from a health/allied health background and total GAMSAT score as consistent independent predictors of stronger academic performance as measured by the weighted average mark for the core units completed throughout the course. The Interview score only weakly predicted performance later in the course and mainly in clinically-based units. The association of total GAMSAT score with academic performance was predominantly dictated by the score in GAMSAT Section 3 (Reasoning in the biological and physical sciences) with Section 1 (Reasoning in the

  20. Fluctuations of Matrix Entries of Regular Functions of Wigner Matrices

    CERN Document Server

    Pizzo, Alessandro; Soshnikov, Alexander

    2011-01-01

    We study the fluctuations of the matrix entries of regular functions of Wigner random matrices in the limit when the matrix size goes to infinity. In the case of the Gaussian ensembles (GOE and GUE) this problem was considered by A.Lytova and L.Pastur in J. Stat. Phys., v.134, 147-159 (2009). Our results are valid provided the off-diagonal matrix entries have finite fourth moment, the diagonal matrix entries have finite third moment, and the test functions have four continuous derivatives in a neighborhood of the support of the Wigner semicircle law.

  1. Japanese subsidiaries in the European Union: Entry modes and performance

    Directory of Open Access Journals (Sweden)

    David Tanganelli

    2014-12-01

    Full Text Available Japanese Foreign Direct Investment (FDI in the European Union and its performance were analysed in this work. Three different FDI or entry modes used by Japanese companies to enter the European market were compared, and the presence of a relationship between the selected entry mode and the performance of the subsidiary was investigated. We found that more than half of the Japanese investments in Europe took the form of new ventures, approximately 40% were joint ventures and less than 6% were acquisitions. We found that no specific entry mode performed better than another.

  2. Convective and radiative heating of a Saturn entry probe

    Science.gov (United States)

    Tiwari, S. N.; Szema, K. Y.; Moss, J. N.; Subramanian, S. V.

    1984-01-01

    The extent of convective and radiative heating for a Saturn entry probe is investigated in the absence and presence of ablation mass injection. The flow in the shock layer is assumed to be axisymmetric, viscous and in local thermodynamic equilibrium. The importance of chemical nonequilibrium effects for both the radiative and convective nonblowing surface heating rates is demonstrated for prescribed entry conditions. Results indicate that the nonequilibrium chemistry can significantly influence the rate of radiative heating to the entry probes. With coupled carbon-phenolic ablation injection, the convective heating rates are reduced substantially. Turbulence has little effect on radiative heating but it increases the convective heating considerably.

  3. Lamiridosins, hepatitis C virus entry inhibitors from Lamium album.

    Science.gov (United States)

    Zhang, Hongjie; Rothwangl, Katharina; Mesecar, Andrew D; Sabahi, Ali; Rong, Lijun; Fong, Harry H S

    2009-12-01

    Phytochemical study of the aqueous extract of the flowering tops of Lamium album led to identification of the antiviral iridoid isomers lamiridosins A and B (1, 2). These compounds were found to significantly inhibit hepatitis C virus entry (IC(50) 2.31 muM) in vitro. Studies of 14 iridoid analogues showed that, while the parent iridoid glucosides demonstrated no anti-HCV entry activity, the aglycones of shanzhiside methyl ester (4), loganin (5), loganic acid (6), geniposide (10), verbenalin (12), eurostoside (15), and picroside II (17) exhibited significant anti-HCV entry and anti-infectivity activities.

  4. Comparable stocks, boundedly rational stock markets and IPO entry rates.

    Directory of Open Access Journals (Sweden)

    Jay Chok

    Full Text Available In this study, we examine how initial public offerings (IPO entry rates are affected when stock markets are boundedly rational and IPO firms infer information from their counterparts in the market. We hypothesize a curvilinear relationship between the number of comparable stocks and initial public offerings (IPO entry rates into the NASDAQ Stock Exchange. Furthermore, we argue that trading volume and changes in stock returns partially mediates the relationship between the number of comparable stocks and IPO entry rates. The statistical evidence provides strong support for the hypotheses.

  5. Physics-Based Modeling of Meteor Entry and Breakup

    Science.gov (United States)

    Prabhu, Dinesh K.; Agrawal, Parul; Allen, Gary A., Jr.; Bauschlicher, Charles W., Jr.; Brandis, Aaron M.; Chen, Yih-Kang; Jaffe, Richard L.; Palmer, Grant E.; Saunders, David A.; Stern, Eric C.; Tauber, Michael E.; Venkatapathy, Ethiraj

    2015-01-01

    A new research effort at NASA Ames Research Center has been initiated in Planetary Defense, which integrates the disciplines of planetary science, atmospheric entry physics, and physics-based risk assessment. This paper describes work within the new program and is focused on meteor entry and breakup.Over the last six decades significant effort was expended in the US and in Europe to understand meteor entry including ablation, fragmentation and airburst (if any) for various types of meteors ranging from stony to iron spectral types. These efforts have produced primarily empirical mathematical models based on observations. Weaknesses of these models, apart from their empiricism, are reliance on idealized shapes (spheres, cylinders, etc.) and simplified models for thermal response of meteoritic materials to aerodynamic and radiative heating. Furthermore, the fragmentation and energy release of meteors (airburst) is poorly understood.On the other hand, flight of human-made atmospheric entry capsules is well understood. The capsules and their requisite heatshields are designed and margined to survive entry. However, the highest speed Earth entry for capsules is 13 kms (Stardust). Furthermore, Earth entry capsules have never exceeded diameters of 5 m, nor have their peak aerothermal environments exceeded 0.3 atm and 1 kW/sq cm. The aims of the current work are: (i) to define the aerothermal environments for objects with entry velocities from 13 to 20 kms; (ii) to explore various hypotheses of fragmentation and airburst of stony meteors in the near term; (iii) to explore the possibility of performing relevant ground-based tests to verify candidate hypotheses; and (iv) to quantify the energy released in airbursts. The results of the new simulations will be used to anchor said risk assessment analyses. With these aims in mind, state-of-the-art entry capsule design tools are being extended for meteor entries. We describe: (i) applications of current simulation tools to

  6. Modulation of Calcium Entry by Mitochondria.

    Science.gov (United States)

    Fonteriz, Rosalba; Matesanz-Isabel, Jessica; Arias-Del-Val, Jessica; Alvarez-Illera, Pilar; Montero, Mayte; Alvarez, Javier

    2016-01-01

    The role of mitochondria in intracellular Ca(2+) signaling relies mainly in its capacity to take up Ca(2+) from the cytosol and thus modulate the cytosolic [Ca(2+)]. Because of the low Ca(2+)-affinity of the mitochondrial Ca(2+)-uptake system, this organelle appears specially adapted to take up Ca(2+) from local high-Ca(2+) microdomains and not from the bulk cytosol. Mitochondria would then act as local Ca(2+) buffers in cellular regions where high-Ca(2+) microdomains form, that is, mainly close to the cytosolic mouth of Ca(2+) channels, both in the plasma membrane and in the endoplasmic reticulum (ER). One of the first targets proposed already in the 1990s to be regulated in this way by mitochondria were the store-operated Ca(2+) channels (SOCE). Mitochondria, by taking up Ca(2+) from the region around the cytosolic mouth of the SOCE channels, would prevent its slow Ca(2+)-dependent inactivation, thus keeping them active for longer. Since then, evidence for this mechanism has accumulated mainly in immunitary cells, where mitochondria actually move towards the immune synapse during T cell activation. However, in many other cell types the available data indicate that the close apposition between plasma and ER membranes occurring during SOCE activation precludes mitochondria from getting close to the Ca(2+)-entry sites. Alternative pathways for mitochondrial modulation of SOCE, both Ca(2+)-dependent and Ca(2+)-independent, have also been proposed, but further work will be required to elucidate the actual mechanisms at work. Hopefully, the recent knowledge of the molecular nature of the mitochondrial Ca(2+) uniporter will allow soon more precise studies on this matter.

  7. The retinoblastoma family of proteins and their regulatory functions in the mammalian cell division cycle

    Directory of Open Access Journals (Sweden)

    Henley Shauna A

    2012-03-01

    Full Text Available Abstract The retinoblastoma (RB family of proteins are found in organisms as distantly related as humans, plants, and insects. These proteins play a key role in regulating advancement of the cell division cycle from the G1 to S-phases. This is achieved through negative regulation of two important positive regulators of cell cycle entry, E2F transcription factors and cyclin dependent kinases. In growth arrested cells transcriptional activity by E2Fs is repressed by RB proteins. Stimulation of cell cycle entry by growth factor signaling leads to activation of cyclin dependent kinases. They in turn phosphorylate and inactivate the RB family proteins, leading to E2F activation and additional cyclin dependent kinase activity. This propels the cell cycle irreversibly forward leading to DNA synthesis. This review will focus on the basic biochemistry and cell biology governing the regulation and activity of mammalian RB family proteins in cell cycle control.

  8. Endotoxin Elimination in Patients with Septic Shock: An Observation Study.

    Science.gov (United States)

    Adamik, Barbara; Zielinski, Stanislaw; Smiechowicz, Jakub; Kübler, Andrzej

    2015-12-01

    To evaluate the effectiveness of endotoxin elimination with an adsorption column in patients with septic shock and endotoxemia. The elimination therapy was guided by a new bedside method of measuring endotoxin activity (EA). Intensive care unit (ICU) patients with septic shock and suspected Gram-negative infection were consecutively added to the study group within the first 24 h. Endotoxin elimination was performed using hemoperfusion with the Alteco LPS Adsorber. The primary endpoint was improvement in organ function within the first 24 h of treatment. A secondary objective was to assess the usefulness of a new method of measuring EA to help guide endotoxin elimination therapy. Out of 64 patients 18 had a high baseline EA [0.70 EA units (0.66-0.77)]. Those patients had endotoxin elimination treatment in addition to conventional medical therapy. At 24 h after endotoxin elimination, the EA had decreased to 0.56 EA units (0.43-0.77), (p = 0.005); MAP increased from 69 (62-80) to 80 mm Hg (68-88), (p = 0.002), and noradrenaline use decreased from 0.28 (0.15-0.80) to 0.1 μg/kg/min (0.00-0.70) at the same time (p = 0.04). The SOFA score had decreased from 11 (9-15) to 9 (7-14) points 24 h after endotoxin elimination (p = 0.01) with a median delta SOFA -2 points. Endotoxin elimination did not have a significant effect on the ICU length of stay or ICU mortality. Effective endotoxin elimination resulted in a significant improvement in hemodynamic parameters and of organ function. The application of the EA assay was useful for the bedside monitoring of endotoxemia in critically ill ICU patients.

  9. The Solar Cycle

    CERN Document Server

    Hathaway, David H

    2015-01-01

    The Solar Cycle is reviewed. The 11-year cycle of solar activity is characterized by the rise and fall in the numbers and surface area of sunspots. A number of other solar activity indicators also vary in association with the sunspots including; the 10.7cm radio flux, the total solar irradiance, the magnetic field, flares and coronal mass ejections, geomagnetic activity, galactic cosmic ray fluxes, and radioisotopes in tree rings and ice cores. Individual solar cycles are characterized by their maxima and minima, cycle periods and amplitudes, cycle shape, the equatorward drift of the active latitudes, hemispheric asymmetries, and active longitudes. Cycle-to-cycle variability includes the Maunder Minimum, the Gleissberg Cycle, and the Gnevyshev-Ohl (even-odd) Rule. Short-term variability includes the 154-day periodicity, quasi-biennial variations, and double-peaked maxima. We conclude with an examination of prediction techniques for the solar cycle and a closer look at cycles 23 and 24.

  10. The Solar Cycle.

    Science.gov (United States)

    Hathaway, David H

    The solar cycle is reviewed. The 11-year cycle of solar activity is characterized by the rise and fall in the numbers and surface area of sunspots. A number of other solar activity indicators also vary in association with the sunspots including; the 10.7 cm radio flux, the total solar irradiance, the magnetic field, flares and coronal mass ejections, geomagnetic activity, galactic cosmic ray fluxes, and radioisotopes in tree rings and ice cores. Individual solar cycles are characterized by their maxima and minima, cycle periods and amplitudes, cycle shape, the equatorward drift of the active latitudes, hemispheric asymmetries, and active longitudes. Cycle-to-cycle variability includes the Maunder Minimum, the Gleissberg Cycle, and the Gnevyshev-Ohl (even-odd) Rule. Short-term variability includes the 154-day periodicity, quasi-biennial variations, and double-peaked maxima. We conclude with an examination of prediction techniques for the solar cycle and a closer look at cycles 23 and 24.

  11. Hepatitis C virus depends on E-cadherin as an entry factor and regulates its expression in epithelial-to-mesenchymal transition.

    Science.gov (United States)

    Li, Qisheng; Sodroski, Catherine; Lowey, Brianna; Schweitzer, Cameron J; Cha, Helen; Zhang, Fang; Liang, T Jake

    2016-07-01

    Hepatitis C virus (HCV) enters the host cell through interactions with a cascade of cellular factors. Although significant progress has been made in understanding HCV entry, the precise mechanisms by which HCV exploits the receptor complex and host machinery to enter the cell remain unclear. This intricate process of viral entry likely depends on additional yet-to-be-defined cellular molecules. Recently, by applying integrative functional genomics approaches, we identified and interrogated distinct sets of host dependencies in the complete HCV life cycle. Viral entry assays using HCV pseudoparticles (HCVpps) of various genotypes uncovered multiple previously unappreciated host factors, including E-cadherin, that mediate HCV entry. E-cadherin silencing significantly inhibited HCV infection in Huh7.5.1 cells, HepG2/miR122/CD81 cells, and primary human hepatocytes at a postbinding entry step. Knockdown of E-cadherin, however, had no effect on HCV RNA replication or internal ribosomal entry site (IRES)-mediated translation. In addition, an E-cadherin monoclonal antibody effectively blocked HCV entry and infection in hepatocytes. Mechanistic studies demonstrated that E-cadherin is closely associated with claudin-1 (CLDN1) and occludin (OCLN) on the cell membrane. Depletion of E-cadherin drastically diminished the cell-surface distribution of these two tight junction proteins in various hepatic cell lines, indicating that E-cadherin plays an important regulatory role in CLDN1/OCLN localization on the cell surface. Furthermore, loss of E-cadherin expression in hepatocytes is associated with HCV-induced epithelial-to-mesenchymal transition (EMT), providing an important link between HCV infection and liver cancer. Our data indicate that a dynamic interplay among E-cadherin, tight junctions, and EMT exists and mediates an important function in HCV entry.

  12. 19 CFR 122.26 - Entry and clearance.

    Science.gov (United States)

    2010-04-01

    ... AIR COMMERCE REGULATIONS Private Aircraft § 122.26 Entry and clearance. Private aircraft, as defined... information as set forth in § 122.22(c), and grants electronic clearance via electronic mail or telephone....

  13. 27 CFR 22.25 - Right of entry and examination.

    Science.gov (United States)

    2010-04-01

    ... BUREAU, DEPARTMENT OF THE TREASURY LIQUORS DISTRIBUTION AND USE OF TAX-FREE ALCOHOL Administrative Provisions Authorities § 22.25 Right of entry and examination. An appropriate TTB officer may enter,...

  14. Multidisciplinary Design Under Uncertainty for Entry Vehicles Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The physical difficulty of designing entry vehicles originates from the large degree of coupling between the various disciplines involved in the design. Every...

  15. Higher Entry Threshold of the Secondary Lead Industry

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    <正>According to news posted on the Ministry of Industry and Information Technology’s website on September 5,the MIIT and the Ministry of Environmental Protection recently jointly issued the Entry Conditions of the Secondary

  16. Orion Entry Performance-Based Center-of-Gravity Box

    Science.gov (United States)

    Rea, Jeremy R.

    2010-01-01

    The Orion capsule has many performance requirements for its atmospheric entry trajectory. Requirements on landing accuracy, maximum heating rate, total heat load, propellant usage, and sensed acceleration must all be satised. It is desired to define a methodology to translate the many performance requirements for an atmospheric entry trajectory into language easily understood by vehicle designers in terms of an allowable center-of-gravity box. This is possible by noting that most entry performance parameters for a capsule vehicle are mainly determined by the lift-to-drag ratio of the vehicle. However, the lift-to- drag ratio should be considered a probabilistic quantity rather than deterministic, where variations in the lift-to-drag are caused by both aerodynamic and center-of-gravity un- certainties. This paper discusses the technique used by the Orion program to define the allowable dispersions in center-of-gravity to achieve the desired entry performance while accounting for aerodynamic uncertainty.

  17. 33 CFR 151.08 - Denial of entry.

    Science.gov (United States)

    2010-07-01

    ... BALLAST WATER Implementation of MARPOL 73/78 and the Protocol on Environmental Protection to the Antarctic Treaty as it Pertains to Pollution from Ships General § 151.08 Denial of entry. (a) Unless a ship...

  18. The state-of-the-art port of entry workshop

    Energy Technology Data Exchange (ETDEWEB)

    Godfrey, B.

    1995-05-01

    The increased demand for freight movements through international ports of entry and the signing of the North American Free Trade Agreement (NAFTA) have increased freight traffic at border ports of entry. The State-of-the-Art Port of Entry Workshop initiated a dialogue among technologists and stakeholders to explore the potential uses of technology at border crossings and to set development priorities. International ports of entry are both information and labor intensive, and there are many promising technologies that could be used to provide timely information and optimize inspection resources. Participants universally held that integration of technologies and operations is critical to improving port services. A series of Next Steps was developed to address stakeholder issues and national priorities, such as the National Transportation Policy and National Drug Policy. This report documents the views of the various stakeholders and technologists present at the workshop and outlines future directions of study.

  19. Adapting Mars Entry, Descent and Landing System for Earth

    Science.gov (United States)

    Heilimo, J.; Harri, A.-M.; Aleksashkin, S.; Koryanov, V.; Guerrero, H.; Schmidt, W.; Haukka, H.; Finchenko, V.; Martynov, M.; Ostresko, B.; Ponomarenko, A.; Kazakovtsev, V.; Arruego, I.; Martin, S.; Siili, T.

    2013-09-01

    In 2001 - 2011 an inflatable Entry, Descent and Landing System (EDLS) for Martian atmosphere was developed by FMI and the MetNet team. This MetNet Mars Lander EDLS is used in both the initial deceleration during atmospheric entry and in the final deceleration before the semi-hard impact of the penetrator to Martian surface. The EDLS design is ingenious and its applicability to Earth's atmosphere is studied in the on-going project. In particular, the behavior of the system in the critical transonic aerodynamic (from hypersonic to subsonic) regime will be investigated. This project targets to analyze and test the transonic behavior of this compact and light weight payload entry system to Earth's atmosphere [1]. Scaling and adaptation for terrestrial atmospheric conditions, instead of a completely new design, is a favorable approach for providing a new re-entry vehicle for terrestrial space applications.

  20. Entry Vehicle Control System Design for the Mars Smart Lander

    Science.gov (United States)

    Calhoun, Philip C.; Queen, Eric M.

    2002-01-01

    The NASA Langley Research Center, in cooperation with the Jet Propulsion Laboratory, participated in a preliminary design study of the Entry, Descent and Landing phase for the Mars Smart Lander Project. This concept utilizes advances in Guidance, Navigation and Control technology to significantly reduce uncertainty in the vehicle landed location on the Mars surface. A candidate entry vehicle controller based on the Reaction Control System controller for the Apollo Lunar Excursion Module digital autopilot is proposed for use in the entry vehicle attitude control. A slight modification to the phase plane controller is used to reduce jet-firing chattering while maintaining good control response for the Martian entry probe application. The controller performance is demonstrated in a six-degree-of-freedom simulation with representative aerodynamics.

  1. Multidisciplinary Design Under Uncertainty for Entry Vehicles Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The physical difficulty of designing entry vehicles originates from the large degree of coupling between the various disciplines involved in the design. Every...

  2. Advanced Metal Rubber Sensors for Hypersonic Decelerator Entry Systems Project

    Data.gov (United States)

    National Aeronautics and Space Administration — NanoSonic proposes to design and develop light-weight, low-modulus, and durable Metal Rubber™ sensors for aeroelastic analysis of Hypersonic Decelerator Entry...

  3. Two inhibitory systems and CKIs regulate cell cycle exit of mammalian cardiomyocytes after birth

    Energy Technology Data Exchange (ETDEWEB)

    Tane, Shoji; Okayama, Hitomi; Ikenishi, Aiko; Amemiya, Yuki [School of Life Sciences, Faculty of Medicine, Tottori University, Yonago 683-8503 (Japan); Nakayama, Keiichi I. [Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582 (Japan); Takeuchi, Takashi, E-mail: takeuchi@med.tottori-u.ac.jp [School of Life Sciences, Faculty of Medicine, Tottori University, Yonago 683-8503 (Japan)

    2015-10-16

    Mammalian cardiomyocytes actively proliferate during embryonic stages, following which they exit their cell cycle after birth, and the exit is maintained. Previously, we showed that two inhibitory systems (the G1-phase inhibitory system: repression of cyclin D1 expression; the M-phase inhibitory system: inhibition of CDK1 activation) maintain the cell cycle exit of mouse adult cardiomyocytes. We also showed that two CDK inhibitors (CKIs), p21{sup Cip1} and p27{sup Kip1}, regulate the cell cycle exit in a portion of postnatal cardiomyocytes. It remains unknown whether the two inhibitory systems are involved in the cell cycle exit of postnatal cardiomyocytes and whether p21{sup Cip1} and p27{sup Kip1} also inhibit entry to M-phase. Here, we showed that more than 40% of cardiomyocytes entered an additional cell cycle by induction of cyclin D1 expression at postnatal stages, but M-phase entry was inhibited in the majority of cardiomyocytes. Marked cell cycle progression and endoreplication were observed in cardiomyocytes of p21{sup Cip1} knockout mice at 4 weeks of age. In addition, tri- and tetranucleated cardiomyocytes increased significantly in p21{sup Cip1} knockout mice. These data showed that the G1-phase inhibitory system and two CKIs (p21{sup Cip1} and p27{sup Kip1}) inhibit entry to an additional cell cycle in postnatal cardiomyocytes, and that the M-phase inhibitory system and p21{sup Cip1} inhibit M-phase entry of cardiomyocytes which have entered the additional cell cycle. - Highlights: • Many postnatal cardiomyocytes entered an additional cell cycle by cyclin D1 induction. • The majority of cardiomyocytes could not enter M-phase after cyclin D1 induction. • Cell cycle progressed markedly in p21{sup Cip1} knockout mice after postnatal day 14. • Tri- and tetranucleated cardiomyocytes increased in p21{sup Cip1} knockout mice.

  4. Simulation of the ATV Re-Entry Obsrvations

    Science.gov (United States)

    Bastida Virgili, B.; Krag, H.; Lips, T.; De Pasquale, E.

    2010-09-01

    The first ATV was launched on 9th March 2008 and, after a successful mission, the last phase was a controlled destructive re-entry on 29th September 2008, shortly after 13:30 UTC, in which the remains of the ATV and its load fell into the South Pacific Ocean. In order to better understand the re-entry processes, an insitu optical observation campaign was launched to record and analyze the ATV controlled re-entry with several instruments on board of two airplanes and also from the ISS. This observation campaign was successful and triggered several different still-ongoing studies on the extraction and analysis of data to draw conclusions on the adequacy of the re-entry break-up and explosion models used for the safety analysis of the ATV re-entry. This paper addresses the validation process for ESA’s model for re-entry survivability and on-ground risk assessment for explosive re-entry events using the observation data. The underlying rationale is to improve the models for the benefit of planning and execution of future controlled re-entries and in risk calculation in case of uncontrolled ones. The re-entry trajectory of the ATV, the explosive event and the trajectories of the fragments are simulated with the existing ESA tools and the EVOLVE explosion model. Additional software has been developed to simulate airborne sensor field of view(FOV) crossings based on the aircraft trajectories, attitude profile, sensor mounts and FOVs. Sensor performance and object radiation are modeled in order to generate synthetic images for the different sensors in the ISS and the two airplanes. These synthetic images and synthetic videos are compared with the available reentry observations of the ATV. This paper will present the software and techniques to generate synthetic imagery. It will give results of the comparison between the simulated and the real trajectories and fragmentation and explain the subsequent validation process of the ESA re-entry tools and the potential

  5. Re-entry simulation chamber for thermo-mechanical characterisation of space materials

    Science.gov (United States)

    Liedtke, Volker

    2003-09-01

    During re-entry, materials and components are subject to very high thermal and mechanical loads. Any failure may cause loss of mission. Therefore, materials and components have to be tested under most rigid conditions to verify the suitability of the material and to verify the design of the components. The Re-Entry Simulation Chamber (RESiC) at ARC Seibersdorf research (ARCS) allows simulating the high thermal loads as well as complex mechanical load profiles that may occur during a re-entry; additionally, the influence of chemical reactions of materials with gaseous components of the atmosphere can be studied. The high vacuum chamber (better than 1×10-6 mbar) has a diameter of 650 mm and allows a sample height of 500 mm, or 1000 mm with extension flange. The gas dosing system is designed to emulate the increasing atmospheric pressure during the re-entry trajectory of a vehicle. Heating is performed by a 30 kW induction generator that allows a sufficiently rapid heating of larger components; electrically conductive materials such as metals or carbon fibre reinforced ceramics are directly heated, while for electrical insulators, susceptor plates or tubes will be employed. The uniaxial servo-hydraulic testing machine has a maximum load of 70 kN, either static or with a frequency of up to 70 Hz, with any given load profile (sinus, rectangular, triangular, ...). Strain measurements will be done by non-contacting laser speckle system for maximum flexibility and minimum instrumentation time effort (currently under application testing), or by strain gauges. All relevant process parameters are controlled and recorded by microcomputer. The highly sophisticated control software allows a convenient and reliable multi-channel data acquisition, e.g. temperatures at various positions of the test piece, pressure, loads, strains, and any other test data according to customer specifications; the data format is suitable for any further data processing. During the set-up and

  6. FDI In India : Determinents Of Entry Modes In India

    OpenAIRE

    Jain, Gaurav

    2003-01-01

    Although cultural distance between countries have gained a lot of attention in the FDI literature but impact on cultural distance on entry mode has recently been extensively researched, after Kogut and Singh introduced the simple measure to calculate cultural distance between the countries. This dissertation tests the proposition that do cultural distance, Sector, or Nationality affect the entry mode strategies of multinational firms when they enter foreign countries. I focus my study by conc...

  7. Lipoprotein Receptors Redundantly Participate in Entry of Hepatitis C Virus.

    Directory of Open Access Journals (Sweden)

    Satomi Yamamoto

    2016-05-01

    Full Text Available Scavenger receptor class B type 1 (SR-B1 and low-density lipoprotein receptor (LDLR are known to be involved in entry of hepatitis C virus (HCV, but their precise roles and their interplay are not fully understood. In this study, deficiency of both SR-B1 and LDLR in Huh7 cells was shown to impair the entry of HCV more strongly than deficiency of either SR-B1 or LDLR alone. In addition, exogenous expression of not only SR-B1 and LDLR but also very low-density lipoprotein receptor (VLDLR rescued HCV entry in the SR-B1 and LDLR double-knockout cells, suggesting that VLDLR has similar roles in HCV entry. VLDLR is a lipoprotein receptor, but the level of its hepatic expression was lower than those of SR-B1 and LDLR. Moreover, expression of mutant lipoprotein receptors incapable of binding to or uptake of lipid resulted in no or slight enhancement of HCV entry in the double-knockout cells, suggesting that binding and/or uptake activities of lipid by lipoprotein receptors are essential for HCV entry. In addition, rescue of infectivity in the double-knockout cells by the expression of the lipoprotein receptors was not observed following infection with pseudotype particles bearing HCV envelope proteins produced in non-hepatic cells, suggesting that lipoproteins associated with HCV particles participate in the entry through their interaction with lipoprotein receptors. Buoyant density gradient analysis revealed that HCV utilizes these lipoprotein receptors in a manner dependent on the lipoproteins associated with HCV particles. Collectively, these results suggest that lipoprotein receptors redundantly participate in the entry of HCV.

  8. Lipoprotein Receptors Redundantly Participate in Entry of Hepatitis C Virus.

    Science.gov (United States)

    Yamamoto, Satomi; Fukuhara, Takasuke; Ono, Chikako; Uemura, Kentaro; Kawachi, Yukako; Shiokawa, Mai; Mori, Hiroyuki; Wada, Masami; Shima, Ryoichi; Okamoto, Toru; Hiraga, Nobuhiko; Suzuki, Ryosuke; Chayama, Kazuaki; Wakita, Takaji; Matsuura, Yoshiharu

    2016-05-01

    Scavenger receptor class B type 1 (SR-B1) and low-density lipoprotein receptor (LDLR) are known to be involved in entry of hepatitis C virus (HCV), but their precise roles and their interplay are not fully understood. In this study, deficiency of both SR-B1 and LDLR in Huh7 cells was shown to impair the entry of HCV more strongly than deficiency of either SR-B1 or LDLR alone. In addition, exogenous expression of not only SR-B1 and LDLR but also very low-density lipoprotein receptor (VLDLR) rescued HCV entry in the SR-B1 and LDLR double-knockout cells, suggesting that VLDLR has similar roles in HCV entry. VLDLR is a lipoprotein receptor, but the level of its hepatic expression was lower than those of SR-B1 and LDLR. Moreover, expression of mutant lipoprotein receptors incapable of binding to or uptake of lipid resulted in no or slight enhancement of HCV entry in the double-knockout cells, suggesting that binding and/or uptake activities of lipid by lipoprotein receptors are essential for HCV entry. In addition, rescue of infectivity in the double-knockout cells by the expression of the lipoprotein receptors was not observed following infection with pseudotype particles bearing HCV envelope proteins produced in non-hepatic cells, suggesting that lipoproteins associated with HCV particles participate in the entry through their interaction with lipoprotein receptors. Buoyant density gradient analysis revealed that HCV utilizes these lipoprotein receptors in a manner dependent on the lipoproteins associated with HCV particles. Collectively, these results suggest that lipoprotein receptors redundantly participate in the entry of HCV.

  9. Fractionation and fragmentation of glass cosmic spherules during atmospheric entry

    Digital Repository Service at National Institute of Oceanography (India)

    Rudraswami, N.G.; ShyamPrasad, M.; Babu, E.V.S.S.K.; VijayaKumar, T.; Feng, W.; Plane, J.M.C.

    of these elements during atmospheric entry (Fig. 7). However, the bulk chemical compositions of Al, Ca, Mg, Fe are generally CI-like except for some volatile elements, which rules out an achondritic parent body. Taylor et al. (2007) reported anorthite with low... entry with the exception of the particles having high velocities and low zenith angles (Love and Brownlee, 1991; Vondrak et al., 2008). Larger micrometeorites have undergone ablation leading to siderophile and volatile element depletion, and mass...

  10. The choice of foreign entry modes in a control perspective

    DEFF Research Database (Denmark)

    Boyd, Britta; Dyhr Ulrich, Anna Marie; Hollensen, Svend

    The aim of this article is to investigate the choice of entry modes for international markets in a control perspective. A survey from The Confederation of Danish Industry with 234 Danish small- and medium sized enterprises served as a data base. The entry modes are categorized into three groups d...... turnover. The factors: personal networks and the interruption of the international activities were the most significant factors for the choice of intermediate mode (joint ventures and strategic alliances)....

  11. 19 CFR 147.44 - Entry for another fair.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry for another fair. 147.44 Section 147.44 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) TRADE FAIRS Disposition of Articles Entered for Fairs § 147.44 Entry for another fair. Articles entered for a fair which are to...

  12. Entry ramps in the Nagel-Schreckenberg model

    DEFF Research Database (Denmark)

    Pedersen, Morten Monrad; Ruhoff, Peder Thusgaard

    2002-01-01

    This paper describes a way of including entry ramps in the Nagel-Schreckenberg traffic model. The idea is to place what are called shadow cars on a highway next to cars on entry ramps, which enables the drivers to take ramp cars into account. The model is shown to capture important real......-life traffic phenomena that have not been included in previous models. Furthermore, it is demonstrated that the desirable properties of the Nagel-Schreckenberg model are retained....

  13. Fragmentation of weak non-ablating objects during entry

    Energy Technology Data Exchange (ETDEWEB)

    Canavan, G.H.

    1997-04-01

    Fragmentation of objects during entry can be treated with developed models of breakup, fragment cascade, separation, and deceleration. The results reduce to those derived earlier for strong objects. Model predictions are in agreement with the key features of numerical simulations. Model equations can be inverted analytically to infer object size, entry speed, and strength from measurements of peak power and altitude and fragmentation altitude or time.

  14. Using analytic network process for evaluating mobile text entry methods.

    Science.gov (United States)

    Ocampo, Lanndon A; Seva, Rosemary R

    2016-01-01

    This paper highlights a preference evaluation methodology for text entry methods in a touch keyboard smartphone using analytic network process (ANP). Evaluation of text entry methods in literature mainly considers speed and accuracy. This study presents an alternative means for selecting text entry method that considers user preference. A case study was carried out with a group of experts who were asked to develop a selection decision model of five text entry methods. The decision problem is flexible enough to reflect interdependencies of decision elements that are necessary in describing real-life conditions. Results showed that QWERTY method is more preferred than other text entry methods while arrangement of keys is the most preferred criterion in characterizing a sound method. Sensitivity analysis using simulation of normally distributed random numbers under fairly large perturbation reported the foregoing results reliable enough to reflect robust judgment. The main contribution of this paper is the introduction of a multi-criteria decision approach in the preference evaluation of text entry methods.

  15. Effects ok ikea's entry into a furniture production cluster

    Directory of Open Access Journals (Sweden)

    Vasco Eiriz

    2016-04-01

    Full Text Available The entry of a multinational into a cluster, a geographic agglomeration in a given place or region of predominantly small and medium enterprises specialized in a given industry or related industries, impacts the incumbent in the cluster. Aiming to identify the main effects of a multinational entry on the firms’ strategy in a cluster, it was analyzed the entry of IKEA, a Swedish multinational, into the cluster of furniture production in Paços de Ferreira and Paredes, in Portugal. In this study, the data collection technique to access primary data was a survey. The sample has small enterprises, which is similar to the structure of firms in the studied cluster. Results show that more than half the sample thinks that the entry of the multinational had not affected them. However, the firms that acknowledge a significant impact, assess that impact as negative. The competitiveness factors that have improved more significantly after IKEA’s entry were new product development and exporting strategies. The main responses of incumbent firms to the multinational entry were internationalization and the development of generic strategies of differentiation and focus based on differentiation.

  16. Thermal Testing of Woven TPS Materials in Extreme Entry Environments

    Science.gov (United States)

    Gonzales, G.; Stackpoole, M.

    2014-01-01

    NASAs future robotic missions to Venus and outer planets, namely, Saturn, Uranus, Neptune, result in extremely high entry conditions that exceed the capabilities of current mid density ablators (PICA or Avcoat). Therefore mission planners assume the use of a fully dense carbon phenolic heatshield similar to what was flown on Pioneer Venus and Galileo. Carbon phenolic (CP) is a robust TPS however its high density and thermal conductivity constrain mission planners to steep entries, high heat fluxes, high pressures and short entry durations, in order for CP to be feasible from a mass perspective. In 2012 the Game Changing Development Program in NASAs Space Technology Mission Directorate funded NASA ARC to investigate the feasibility of a Woven Thermal Protection System to meet the needs of NASAs most challenging entry missions. The high entry conditions pose certification challenges in existing ground based test facilities. Recent updates to NASAs IHF and AEDCs H3 high temperature arcjet test facilities enable higher heatflux (2000 Wcm2) and high pressure (5 atm) testing of TPS. Some recent thermal tests of woven TPS will be discussed in this paper. These upgrades have provided a way to test higher entry conditions of potential outer planet and Venus missions and provided a baseline against carbon phenolic material. The results of these tests have given preliminary insight to sample configuration and physical recession profile characteristics.

  17. Three-stage entry game: The strategic effects of advertising

    Directory of Open Access Journals (Sweden)

    Kuzmanović Marija

    2011-01-01

    Full Text Available This paper analyzes the effects of investment in advertising in the three-stage entry game model with one incumbent and one potential entrant firm. It is shown that if a game theory is applied, under particular conditions, advertising can be used as a strategic weapon in the market entry game. Depending on the level of the advertising interaction factor, conditions for over-investment in advertising for strategic purposes are given. Furthermore, three specific cases are analyzed: strictly predatory advertising, informative advertising and the case when one firm’s advertising cannot directly influence the other firm's profit. For each of them, depending on the costs of advertising and marginal costs, equilibrium is determined, and conditions under which it is possible to deter the entry are given. It is shown that if the value of the advertising interaction factor increases, power of using advertising as a weapon to deter entry into the market decreases. Thus, in the case of informative advertising, advertising cannot be used as a tool for deterring entry into the market, while in the case of predatory advertising, it can. Also, we have proved that in the case of strictly informative advertising an over-investment never occurs, while in the two other cases, there is always over-investment either to deter or to accommodate the entry.

  18. Inhibition of influenza H7 hemagglutinin-mediated entry.

    Directory of Open Access Journals (Sweden)

    Aleksandar Antanasijevic

    Full Text Available The recent outbreak of H7N9 influenza in China is of high concern to public health. H7 hemagglutinin (HA plays a critical role in influenza entry and thus HA presents an attractive target for antivirals. Previous studies have suggested that the small molecule tert-butyl hydroquinone (TBHQ inhibits the entry of influenza H3 HA by binding to the stem loop of HA and stabilizing the neutral pH conformation of HA, thereby disrupting the membrane fusion step. Based on amino acid sequence, structure and immunogenicity, H7 is a related Group 2 HA. In this work we show, using a pseudovirus entry assay, that TBHQ inhibits H7 HA-mediated entry, as well as H3 HA-mediated entry, with an IC50 ~ 6 µM. Using NMR, we show that TBHQ binds to the H7 stem loop region. STD NMR experiments indicate that the aromatic ring of TBHQ makes extensive contact with the H7 HA surface. Limited proteolysis experiments indicate that TBHQ inhibits influenza entry by stabilizing the H7 HA neutral pH conformation. Together, this work suggests that the stem loop region of H7 HA is an attractive target for therapeutic intervention and that TBHQ, which is a widely used food preservative, is a promising lead compound.

  19. Dynamics of ambulatory surgery centers and hospitals market entry.

    Science.gov (United States)

    Housman, Michael; Al-Amin, Mona

    2013-08-01

    In this article, we investigate the diversity of healthcare delivery organizations by comparing the market determinants of hospitals entry rates with those of ambulatory surgery centers (ASCs). Unlike hospitals, ASCs is one of the growing populations of specialized healthcare delivery organizations. There are reasons to believe that firm entry patterns differ within growing organizational populations since these markets are characterized by different levels of organizational legitimacy, technological uncertainty, and information asymmetry. We compare the entry patterns of firms in a mature population of hospitals to those of firms within a growing population of ASCs. By using patient-level datasets from the state of Florida, we break down our explanatory variables by facility type (ASC vs. hospital) and utilize negative binomial regression models to evaluate the impact of niche density on ASC and hospital entry. Our results indicate that ASCs entry rates is higher in markets with overlapping ASCs while hospitals entry rates are less in markets with overlapping hospitals and ASCs. These results are consistent with the notion that firms in growing populations tend to seek out crowded markets as they compete to occupy the most desirable market segments while firms in mature populations such as general hospitals avoid direct competition.

  20. Entry and Exit Stress Variation of Cold Rolling Strip

    Institute of Scientific and Technical Information of China (English)

    WANGDong—cheng

    2012-01-01

    The shortcomings of an exit stress variation formula which has been well accepted are analyzed~ it is found that the exit stress variation formula violates the premise of the law of volume constancy. The shortcomings of an en- try stress variation formula are analyzed too, and the basic assumption of the formula is uniform exit velocity. How- ever, for a rigid-plastic material uniform exit velocity implies that the lateral distributioi1 of elongation is uniform, so the exit stress must be uniform and any type of flatness defect is impossible, which is contrary to the practice. In fact, entry and exit velocity variation influence entry and exit stress variation, and entry and exit stress variation in- fluence entry and exit velocity variation too, so a precise explicit stress variation formula cannot be got easily. Con- sidering the relationship between stress variation and velocity variation, an iteration method is presented to calculate entry and exit stress variation of cold rolling strip. To avoid divergent phenomenon of the iteration course, a relaxa- tion factor method is adopted. The calculation results are compared with the entry and exit stress variation formula commonly used by many researchers. The difference is remarkable, while the result calculated agree more well with measured result if the exit elastic recovery zone is considered. Specially, the incoming flatnessI propagate efficiency calculated ~ives a more realistic result.

  1. Nursing medication administration and workflow using computerized physician order entry.

    Science.gov (United States)

    Tschannen, Dana; Talsma, Akkeneel; Reinemeyer, Nicholas; Belt, Christine; Schoville, Rhonda

    2011-07-01

    The benefits of computerized physician order entry systems have been described widely; however, the impact of computerized physician order entry on nursing workflow and its potential for error are unclear. The purpose of this study was to determine the impact of a computerized physician order entry system on nursing workflow. Using an exploratory design, nurses employed on an adult ICU (n = 36) and a general pediatric unit (n = 50) involved in computerized physician order entry-based medication delivery were observed. Nurses were also asked questions regarding the impact of computerized physician order entry on nursing workflow. Observations revealed total time required for administering medications averaged 8.45 minutes in the ICU and 9.93 minutes in the pediatric unit. Several additional steps were required in the process for pediatric patients, including preparing the medications and communicating with patients and family, which resulted in greater time associated with the delivery of medications. Frequent barriers to workflow were noted by nurses across settings, including system issues (ie, inefficient medication reconciliation processes, long order sets requiring more time to determine medication dosage), less frequent interaction between the healthcare team, and greater use of informal communication modes. Areas for nursing workflow improvement include (1) medication reconciliation/order duplication, (2) strategies to improve communication, and (3) evaluation of the impact of computerized physician order entry on practice standards.

  2. Acceptions of the "death"entry in language dictionaries

    Directory of Open Access Journals (Sweden)

    Jessica Camara Siqueira

    2013-08-01

    Full Text Available Language dictionaries present the meaning of an entry in a way not restricted to linguistic information, since we also have interdisciplinary and contextual aspects that dialogue with socio-cultural issues in which the entry is inserted. Considering the potential character of the study of contextual meanings, the “death” entry was chosen to carry out a comparative analysis among language dictionaries. The choice was motivated by the recurrence of this entry in ancient and contemporary dictionaries, and the fact of its having a concept that allows various historical, social and ideological discussions. The goal is to reveal the different nuances of the “death” entry in language dictionaries, observing four main aspects: etymology, diachrony, synonymic construction and meaning of “death” in school dictionaries. Based on lexicographical studies of classical authors, we did a comparative analysis of meanings of “death” in different types of language dictionaries. We found that in each group dictionary we may find traces of ideological choices in the construction of the contextual meaning of the “death” entry.

  3. NPDES (National Pollution Discharge & Elimination System) Minor Dischargers

    Data.gov (United States)

    U.S. Environmental Protection Agency — As authorized by the Clean Water Act, the National Pollutant Discharge Elimination System (NPDES) permit program controls water pollution by regulating point sources...

  4. International population movements and regional Plasmodium falciparum malaria elimination strategies

    National Research Council Canada - National Science Library

    Andrew J. Tatem; David L. Smith; Susan Hanson

    2010-01-01

    ... to areas targeted for elimination. Here, census-based migration data were analyzed with network analysis tools, Plasmodium falciparum malaria transmission maps, and global population databases to map globally communities of countries...

  5. Ask Your Dental Hygienist about Understanding and Eliminating Bad Breath

    Science.gov (United States)

    ... eliminate oral malodor. Tongue de- plaquing with tongue scrap¬ers—tools exclusively designed for use on the tongue—is • Rinsing your mouth with water after eating if you aren’t able to ...

  6. Eliminating the empty bus syndrome by understanding dynamic travel behaviour

    CSIR Research Space (South Africa)

    Mokonyama, Mathetha T

    2012-11-01

    Full Text Available -1 The Sustainable Transport and Mobility Handbook South Africa, Vol. 3: the Essential Guide Eliminating the empty bus syndrome by understanding dynamic travel behaviour Mokonyama Mathetha CSIR. Built Environment. PO Box 395, Pretoria, South Africa, 0001...

  7. Innovative first order elimination kinetics working model for easy learning

    Directory of Open Access Journals (Sweden)

    Navin Budania

    2016-06-01

    Conclusions: First order elimination kinetics is easily understood with the help of above working model. More and more working models could be developed for teaching difficult topics. [Int J Basic Clin Pharmacol 2016; 5(3.000: 862-864

  8. Solar cycle 25: another moderate cycle?

    CERN Document Server

    Cameron, Robert H; Schuessler, Manfred

    2016-01-01

    Surface flux transport simulations for the descending phase of cycle 24 using random sources (emerging bipolar magnetic regions) with empirically determined scatter of their properties provide a prediction of the axial dipole moment during the upcoming activity minimum together with a realistic uncertainty range. The expectation value for the dipole moment around 2020 $(2.5\\pm1.1\\,$G) is comparable to that observed at the end of cycle 23 (about $2\\,$G). The empirical correlation between the dipole moment during solar minimum and the strength of the subsequent cycle thus suggests that cycle 25 will be of moderate amplitude, not much higher than that of the current cycle. However, the intrinsic uncertainty of such predictions resulting from the random scatter of the source properties is considerable and fundamentally limits the reliability with which such predictions can be made before activity minimum is reached.

  9. Lacunarity Elimination in the Translation of Nonequivalent Juridical Terms

    Directory of Open Access Journals (Sweden)

    Vladimir A. Lazarev

    2017-06-01

    Full Text Available In the article the authors refer to the problem of lacunarity elimination in legal texts reflecting national specific features in the legal sphere. Various ways of filling gaps when translating from English into Russian are suggested using legal-dogmatic theoretical texts as an example. Different variants of the lacunae elimination are proposed, which take into account the peculiarities of this type of text and can assist in the work of a legal translator.

  10. Targeting Quiescent Cancer Cells to Eliminate Tumor Recurrence After Therapy

    Science.gov (United States)

    2015-10-01

    AD_________________ (Leave blank) Award Number: W81XWH-14-1-0350 TITLE: Targeting Quiescent Cancer Cells to Eliminate Tumor Recurrence After...30 Sep 2014 - 29 Sep 2015 4. TITLE AND SUBTILE Targeting Quiescent Cancer Cells to Eliminate Tumor Recurrence After Therapy 5a. CONTRACT NUMBER...Innovative reporter gene systems are designed to mark quiescent or proliferating lung cancer cells (Aim 1) and then used to track and trace the dynamics of

  11. Targeting Quiescent Cancer Cells to Eliminate Tumor Recurrence After Therapy

    Science.gov (United States)

    2016-12-01

    AD_________________ Award Number: W81XWH-14-1-0350 TITLE: Targeting Quiescent Cancer Cells to Eliminate Tumor Recurrence After Therapy PRINCIPAL...30 Sep 2014 - 29 Sep 2016 4. TITLE AND SUBTILE Targeting Quiescent Cancer Cells to Eliminate Tumor Recurrence After Therapy 5a. CONTRACT NUMBER... cancer . To eradicate chemoresistant tumor cells, it is important to identify the subset of tumor cells that can survive from chemotherapy and

  12. Proliferation in cycle

    Energy Technology Data Exchange (ETDEWEB)

    Piao Yunsong [College of Physical Sciences, Graduate School of Chinese Academy of Sciences, Beijing 100049 (China)], E-mail: yspiao@gucas.ac.cn

    2009-06-15

    In the contracting phase with w{approx_equal}0, the scale invariant spectrum of curvature perturbation is given by the increasing mode of metric perturbation. In this Letter, it is found that if the contracting phase with w{approx_equal}0 is included in each cycle of a cycle universe, since the metric perturbation is amplified on super horizon scale cycle by cycle, after each cycle the universe will be inevitably separated into many parts independent of one another, each of which corresponds to a new universe and evolves up to next cycle, and then is separated again. In this sense, a cyclic multiverse scenario is actually presented, in which the universe proliferates cycle by cycle. We estimate the number of new universes proliferated in each cycle, and discuss the implications of this result.

  13. Development of a Direct Evaporator for the Organic Rankine Cycle

    Energy Technology Data Exchange (ETDEWEB)

    Donna Post Guillen; Helge Klockow; Matthew Lehar; Sebastian Freund; Jennifer Jackson

    2011-02-01

    This paper describes research and development currently underway to place the evaporator of an Organic Rankine Cycle (ORC) system directly in the path of a hot exhaust stream produced by a gas turbine engine. The main goal of this research effort is to improve cycle efficiency and cost by eliminating the usual secondary heat transfer loop. The project’s technical objective is to eliminate the pumps, heat exchangers and all other added cost and complexity of the secondary loop by developing an evaporator that resides in the waste heat stream, yet virtually eliminates the risk of a working fluid leakage into the gaseous exhaust stream. The research team comprised of Idaho National Laboratory and General Electric Company engineers leverages previous research in advanced ORC technology to develop a new direct evaporator design that will reduce the ORC system cost by up to 15%, enabling the rapid adoption of ORCs for waste heat recovery.

  14. How to achieve customer service through short-cycle paperwork.

    Science.gov (United States)

    Hunter, M

    1998-02-01

    The ultimate goal of short-cycle paperwork is to satisfy customers by filling their orders as quickly as possible. Tools and techniques that can help achieve this goal include Just-in-Time paperwork elimination, process mapping, paper flow mapping, function/process mapping, work cells, and electronic kanban. Each of these is described briefly in the article.

  15. Hepatitis C Virus Life Cycle and Lipid Metabolism

    Directory of Open Access Journals (Sweden)

    Costin-Ioan Popescu

    2014-12-01

    Full Text Available Hepatitis C Virus (HCV infects over 150 million people worldwide. In most cases HCV infection becomes chronic, causing liver disease ranging from fibrosis to cirrhosis and hepatocellular carcinoma. HCV affects the cholesterol homeostasis and at the molecular level, every step of the virus life cycle is intimately connected to lipid metabolism. In this review, we present an update on the lipids and apolipoproteins that are involved in the HCV infectious cycle steps: entry, replication and assembly. Moreover, the result of the assembly process is a lipoviroparticle, which represents a peculiarity of hepatitis C virion. This review illustrates an example of an intricate virus-host interaction governed by lipid metabolism.

  16. Generic entry, reformulations and promotion of SSRIs in the US.

    Science.gov (United States)

    Huskamp, Haiden A; Donohue, Julie M; Koss, Catherine; Berndt, Ernst R; Frank, Richard G

    2008-01-01

    Previous research has shown that a manufacturer's promotional strategy for a brand name drug is typically affected by generic entry. However, little is known about how newer strategies to extend patent life, including product reformulation introduction or obtaining approval to market for additional clinical indications, influence promotion. To examine the relationships among promotional expenditures, generic entry, reformulation entry and new indication approval. We used quarterly data on national product-level promotional spending (including expenditures for physician detailing and direct-to-consumer advertising [DTCA], and the retail value of free samples distributed in physician offices) for selective serotonin reuptake inhibitors (SSRIs) over the period 1997-2004. We estimated econometric models of detailing, DTCA and total quarterly promotional expenditures as a function of the timing of generic entry, entry of new product formulations and US FDA approval for new clinical indications for existing medications in the SSRI class. Expenditures by pharmaceutical manufacturers for promotion of antidepressant medications was the main outcome measure. Over the period 1997-2004, there was considerable variation in the composition of promotional expenditures across the SSRIs. Promotional expenditures for the original brand molecule decreased dramatically when a reformulation of the molecule was introduced. Promotional spending (both total and detailing alone) for a specific molecule was generally lower after generic entry than before, although the effect of generic entry on promotional spending appears to be closely linked with the choice of product reformulation strategy pursued by the manufacturer. Detailing expenditures for Paxil were increased after the manufacturer received FDA approval to market the drug for generalized anxiety disorder (GAD), while the likelihood of DTCA outlays for the drug was not changed. In contrast, FDA approval to market Paxil and Zoloft

  17. Virus entry. Lassa virus entry requires a trigger-induced receptor switch.

    Science.gov (United States)

    Jae, Lucas T; Raaben, Matthijs; Herbert, Andrew S; Kuehne, Ana I; Wirchnianski, Ariel S; Soh, Timothy K; Stubbs, Sarah H; Janssen, Hans; Damme, Markus; Saftig, Paul; Whelan, Sean P; Dye, John M; Brummelkamp, Thijn R

    2014-06-27

    Lassa virus spreads from a rodent to humans and can lead to lethal hemorrhagic fever. Despite its broad tropism, chicken cells were reported 30 years ago to resist infection. We found that Lassa virus readily engaged its cell-surface receptor α-dystroglycan in avian cells, but virus entry in susceptible species involved a pH-dependent switch to an intracellular receptor, the lysosome-resident protein LAMP1. Iterative haploid screens revealed that the sialyltransferase ST3GAL4 was required for the interaction of the virus glycoprotein with LAMP1. A single glycosylated residue in LAMP1, present in susceptible species but absent in birds, was essential for interaction with the Lassa virus envelope protein and subsequent infection. The resistance of Lamp1-deficient mice to Lassa virus highlights the relevance of this receptor switch in vivo.

  18. Permissiveness of human hepatocellular carcinoma cell lines for hepatitis C virus entry and replication.

    Science.gov (United States)

    Coto-Llerena, Mairene; Koutsoudakis, George; Boix, Loreto; López-Oliva, Juan Manuel; Caro-Pérez, Noelia; Fernández-Carrillo, Carlos; González, Patricia; Gastaminza, Pablo; Bruix, Jordi; Forns, Xavier; Pérez-Del-Pulgar, Sofía

    2017-07-24

    Hepatitis C virus (HCV) is a globally prevalent pathogen and is associated with high death rates and morbidity. Since its discovery in 1989, HCV research has been impeded by the lack of a robust infectious cell culture system and thus in vitro studies on diverse genetic backgrounds are hampered because of the limited number of hepatoma cell lines which are able to support different aspects of the HCV life cycle. In the current study, we sought to expand the limited number of permissive cells capable of supporting the diverse phases of the HCV life cycle. Initially, we screened a panel of new hepatoma-derived cell lines, designated BCLC-1, -2, -3, -4, -5, -6, -9 and -10 cells, for their ability to express essential HCV receptors and subsequently to support HCV entry by using the well-characterized HCV pseudoparticle system (HCVpp). Apart from BCLC-9, all BCLC cell lines were permissive for HCVpp infection. Next, BCLC cells were subjected to short- and long-term HCV RNA replication studies using HCV subgenomic replicons. Interestingly, only BCLC-1, -5 and -9 cells, supported short-term HCV RNA replication, but the latter were excluded from further studies since they were refractory for HCV entry. BCLC-1, -5 were able to support long-term HCV replication too; yet BCLC-5 cells supported the highest long-term HCV RNA replication levels. Furthermore, cured BCLC-5 clones from HCV subgenomic replicon, showed increased permissiveness for HCV RNA replication. Strikingly, we were unable to detect endogenous BCLC-5 miR122 expression - an important HCV host factor- and as expected, the exogenous expression of miR122 in BCLC-5 cells increased their permissiveness for HCV RNA replication. However, this cell line was unable to produce HCV infectious particles despite ectopic expression of apolipoprotein E, which in other hepatoma cell lines has been shown to be sufficient to enable the HCV secretion process, suggesting a lack of other host cellular factor(s) and/or the presence of

  19. Cycling in Sydney, Australia

    Directory of Open Access Journals (Sweden)

    Alexis Zander

    2013-01-01

    Full Text Available Introduction. Cycling can be an enjoyable way to meet physical activity recommendations and is suitable for older people; however cycling participation by older Australians is low. This qualitative study explored motivators, enablers, and barriers to cycling among older people through an age-targeted cycling promotion program. Methods. Seventeen adults who aged 50–75 years participated in a 12-week cycling promotion program which included a cycling skills course, mentor, and resource pack. Semistructured interviews at the beginning and end of the program explored motivators, enablers, and barriers to cycling. Results. Fitness and recreation were the primary motivators for cycling. The biggest barrier was fear of cars and traffic, and the cycling skills course was the most important enabler for improving participants’ confidence. Reported outcomes from cycling included improved quality of life (better mental health, social benefit, and empowerment and improved physical health. Conclusions. A simple cycling program increased cycling participation among older people. This work confirms the importance of improving confidence in this age group through a skills course, mentors, and maps and highlights additional strategies for promoting cycling, such as ongoing improvement to infrastructure and advertising.

  20. Quality of data entry using single entry, double entry and automated forms processing--an example based on a study of patient-reported outcomes

    DEFF Research Database (Denmark)

    Paulsen, Aksel; Overgaard, Søren; Lauritsen, Jens Martin

    2012-01-01

    The clinical and scientific usage of patient-reported outcome measures is increasing in the health services. Often paper forms are used. Manual double entry of data is defined as the definitive gold standard for transferring data to an electronic format, but the process is laborious. Automated...

  1. Neural stem cell-derived exosomes mediate viral entry

    Directory of Open Access Journals (Sweden)

    Sims B

    2014-10-01

    Full Text Available Brian Sims,1,2,* Linlin Gu,3,* Alexandre Krendelchtchikov,3 Qiana L Matthews3,4 1Division of Neonatology, Department of Pediatrics, 2Department of Cell, Developmental, and Integrative Biology, 3Division of Infectious Diseases, Department of Medicine, 4Center for AIDS Research, University of Alabama at Birmingham, Birmingham, AL, USA *These authors contributed equally to this work Background: Viruses enter host cells through interactions of viral ligands with cellular receptors. Viruses can also enter cells in a receptor-independent fashion. Mechanisms regarding the receptor-independent viral entry into cells have not been fully elucidated. Exosomal trafficking between cells may offer a mechanism by which viruses can enter cells.Methods: To investigate the role of exosomes on cellular viral entry, we employed neural stem cell-derived exosomes and adenovirus type 5 (Ad5 for the proof-of-principle study. Results: Exosomes significantly enhanced Ad5 entry in Coxsackie virus and adenovirus receptor (CAR-deficient cells, in which Ad5 only had very limited entry. The exosomes were shown to contain T-cell immunoglobulin mucin protein 4 (TIM-4, which binds phosphatidylserine. Treatment with anti-TIM-4 antibody significantly blocked the exosome-mediated Ad5 entry.Conclusion: Neural stem cell-derived exosomes mediated significant cellular entry of Ad5 in a receptor-independent fashion. This mediation may be hampered by an antibody specifically targeting TIM-4 on exosomes. This set of results will benefit further elucidation of virus/exosome pathways, which would contribute to reducing natural viral infection by developing therapeutic agents or vaccines. Keywords: neural stem cell-derived exosomes, adenovirus type 5, TIM-4, viral entry, phospholipids

  2. Improving laboratory data entry quality using Six Sigma.

    Science.gov (United States)

    Elbireer, Ali; Le Chasseur, Julie; Jackson, Brooks

    2013-01-01

    The Uganda Makerere University provides clinical laboratory support to over 70 clients in Uganda. With increased volume, manual data entry errors have steadily increased, prompting laboratory managers to employ the Six Sigma method to evaluate and reduce their problems. The purpose of this paper is to describe how laboratory data entry quality was improved by using Six Sigma. The Six Sigma Quality Improvement (QI) project team followed a sequence of steps, starting with defining project goals, measuring data entry errors to assess current performance, analyzing data and determining data-entry error root causes. Finally the team implemented changes and control measures to address the root causes and to maintain improvements. Establishing the Six Sigma project required considerable resources and maintaining the gains requires additional personnel time and dedicated resources. After initiating the Six Sigma project, there was a 60.5 percent reduction in data entry errors from 423 errors a month (i.e. 4.34 Six Sigma) in the first month, down to an average 166 errors/month (i.e. 4.65 Six Sigma) over 12 months. The team estimated the average cost of identifying and fixing a data entry error to be $16.25 per error. Thus, reducing errors by an average of 257 errors per month over one year has saved the laboratory an estimated $50,115 a year. The Six Sigma QI project provides a replicable framework for Ugandan laboratory staff and other resource-limited organizations to promote quality environment. Laboratory staff can deliver excellent care at a lower cost, by applying QI principles. This innovative QI method of reducing data entry errors in medical laboratories may improve the clinical workflow processes and make cost savings across the health care continuum.

  3. Molecular time-course and the metabolic basis of entry into dauer in Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Pan-Young Jeong

    Full Text Available When Caenorhabditis elegans senses dauer pheromone (daumone, signaling inadequate growth conditions, it enters the dauer state, which is capable of long-term survival. However, the molecular pathway of dauer entry in C. elegans has remained elusive. To systematically monitor changes in gene expression in dauer paths, we used a DNA microarray containing 22,625 gene probes corresponding to 22,150 unique genes from C. elegans. We employed two different paths: direct exposure to daumone (Path 1 and normal growth media plus liquid culture (Path 2. Our data reveal that entry into dauer is accomplished through the multi-step process, which appears to be compartmentalized in time and according to metabolic flux. That is, a time-course of dauer entry in Path 1 shows that dauer larvae formation begins at post-embryonic stage S4 (48 h and is complete at S6 (72 h. Our results also suggest the presence of a unique adaptive metabolic control mechanism that requires both stage-specific expression of specific genes and tight regulation of different modes of fuel metabolite utilization to sustain the energy balance in the context of prolonged survival under adverse growth conditions. It is apparent that worms entering dauer stage may rely heavily on carbohydrate-based energy reserves, whereas dauer larvae utilize fat or glyoxylate cycle-based energy sources. We created a comprehensive web-based dauer metabolic database for C. elegans (www.DauerDB.org that makes it possible to search any gene and compare its relative expression at a specific stage, or evaluate overall patterns of gene expression in both paths. This database can be accessed by the research community and could be widely applicable to other related nematodes as a molecular atlas.

  4. Extracts of medicinal herb Sanguisorba officinalis inhibit the entry of human immunodeficiency virus type one.

    Science.gov (United States)

    Liang, Jianguo; Chen, Jianping; Tan, Zhiwu; Peng, Jie; Zheng, Xiao; Nishiura, Kenji; Ng, Jenny; Wang, Zhiyu; Wang, Dongmei; Chen, Zhiwei; Liu, Li

    2013-12-01

    Highly active antiretroviral therapy (HAART) has been successful in reducing HIV-1-associated morbidity and mortality since its introduction in 1996. It, however, fails to eradicate HIV-1 infection thoroughly. The high cost of life-long HAART and the emergence of drug resistance among HIV-1-infected individuals have brought renewed pressure for the discovery of novel antivirals and alternative medicines. Traditional Chinese medicine (TCM) is one of the mainstreams of complementary and alternative medicine, and serves as rich resources for new drug development. Despite almost 100 plant-derived compounds are in clinical trials, few target HIV-1 infection. In this study, we discovered that extract of Sanguisorba officinalis (SOE) has anti-HIV-1 activities. Using a cell-based assay and single-cycle luciferase reporter viruses pseudotyped with envelopes from HIV-1 or control viruses, we found that SOE exhibited significant inhibitory ability against both CCR5 and CXCR4 tropic HIV-1 (ADA and HXB2) with respective IC50 values of 1.91±0.16 μg/ml and 3.70±0.53 μg/ml. Interestingly, SOE also inhibited SIV infection but failed to block vesicular stomatitis virus (VSV), SARS-CoV and influeunza H5N1 pseudoviruses. Furthermore, we showed that SOE had no effects on post-entry events of HIV-1 replication. It blocked entry by acting on viral envelope directly because SOE pre-treatment with the virus but not with cell lines expressing viral receptors showed the maximal inhibitory activity. In addition, SOE was able to inhibit reverse-transcription-inhibitor-resistant viruses (K103N, Y188L, and K103N/Y188L/G190A) and a protease-inhibitor-resistant strain (PI-2840). Our findings demonstrated SOE as a novel and specific entry inhibitor, which shed lights on the discovery of anti-HIV-1 drugs from traditional herbal medicines.

  5. Control of cell cycle and cell growth by molecular chaperones.

    Science.gov (United States)

    Aldea, Martí; Garí, Eloi; Colomina, Neus

    2007-11-01

    Cells adapt their size to both intrinsic and extrinsic demands and, among them, those that stem from growth and proliferation rates are crucial for cell size homeostasis. Here we revisit mechanisms that regulate cell cycle and cell growth in budding yeast. Cyclin Cln3, the most upstream activator of Start, is retained at the endoplasmic reticulum in early G(1) and released by specific chaperones in late G(1) to initiate the cell cycle. On one hand, these chaperones are rate-limiting for release of Cln3 and cell cycle entry and, on the other hand, they are required for key biosynthetic processes. We propose a model whereby the competition for specialized chaperones between growth and cycle machineries could gauge biosynthetic rates and set a critical size threshold at Start.

  6. Life cycle assessment (LCA)

    DEFF Research Database (Denmark)

    Thrane, Mikkel; Schmidt, Jannick Andresen

    2004-01-01

    The chapter introduces Life Cycle Assessment (LCA) and its application according to the ISO 1404043 standards.......The chapter introduces Life Cycle Assessment (LCA) and its application according to the ISO 1404043 standards....

  7. Life cycle assessment (LCA)

    DEFF Research Database (Denmark)

    Thrane, Mikkel; Schmidt, Jannick Andresen

    2004-01-01

    The chapter introduces Life Cycle Assessment (LCA) and its application according to the ISO 1404043 standards.......The chapter introduces Life Cycle Assessment (LCA) and its application according to the ISO 1404043 standards....

  8. Menstrual Cycle Problems

    Science.gov (United States)

    ... Read MoreDepression in Children and TeensRead MoreBMI Calculator Menstrual Cycle ProblemsFrom missed periods to painful periods, menstrual cycle problems are common, but usually not serious. Follow ...

  9. 14 CFR 121.709 - Airworthiness release or aircraft log entry.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Airworthiness release or aircraft log entry... Airworthiness release or aircraft log entry. (a) No certificate holder may operate an aircraft after maintenance... appropriate entry in the aircraft log. (b) The airworthiness release or log entry required by paragraph (a)...

  10. Clinical relevance of the small intestine as an organ of drug elimination: drug-fruit juice interactions.

    Science.gov (United States)

    Paine, Mary F; Oberlies, Nicholas H

    2007-02-01

    Most drugs are taken orally. For those intended to act systemically, a significant fraction of the dose can be eliminated during its first passage through a sequence of organs before entry into the general circulation. For some drugs, the degree of first-pass elimination can be large enough such that oral bioavailability is significantly reduced, with the consequent potential for a reduced clinical response. Of these first-pass eliminating organs, the small intestine and liver are the most commonly implicated, in part because they express the highest levels of drug-metabolizing enzymes. For several drugs whose major route of elimination occurs via CYP3A-mediated metabolism, the extent of first-pass metabolism in the small intestine can rival that in the liver. As such, alterations in enteric CYP3A activity alone can significantly influence oral bioavailability. The most extensively studied xenobiotic shown to inhibit only enteric CYP3A is grapefruit juice, the consequences of which can be clinically significant. Although much information has been gained regarding the grapefruit juice effect, progress in the relatively understudied area of drug-diet interactions continues to be sluggish and reactive. In stark contrast, the potential for drug-drug interactions involving any new therapeutic agent must be evaluated, prospectively, before market introduction. To prospectively elucidate mechanisms underlying drug-diet interactions, a multidisciplinary, translational research approach is required, which capitalizes on the collective expertise of drug metabolism scientists and natural products chemists. Such an approach would allow proper between-study comparisons, and ultimately provide conclusive information as to whether specific dietary substances can be taken safely with certain medications.

  11. Suppression of EGF-induced cell proliferation by the blockade of Ca2+ mobilization and capacitative Ca2+ entry in mouse mammary epithelial cells.

    Science.gov (United States)

    Ichikawa, J; Kiyohara, T

    2001-09-01

    The role of intracellular Ca2+ stores and capacitative Ca2+ entry on EGF-induced cell proliferation was investigated in mouse mammary epithelial cells. We have previously demonstrated that EGF enhances Ca2+ mobilization (release of Ca2+ from intracellular Ca2+ stores) and capacitative Ca2+ entry correlated with cell proliferation in mouse mammary epithelial cells. To confirm their role on EGF-induced cell cycle progression, we studied the effects of 2,5-di-tert-butylhydroquinone (DBHQ), a reversible inhibitor of the Ca2+ pump of intracellular Ca2+ stores, and SK&F 96365, a blocker of capacitative Ca2+ entry, on mitotic activity induced by EGF. Mitotic activity was examined using an antibody to PCNA for immunocytochemistry. SK&F 96365 inhibited capacitative Ca2+ entry in a dose-dependent manner (I50: 1-5 microM). SK&F 96365 also inhibited EGF-induced cell proliferation in the same range of concentration (I50: 1-5 microM). DBHQ suppressed [Ca2+]i response to UTP and thus depleted completely Ca2+ stores at 5 microM. DBHQ also inhibited EGF-induced cell proliferation at an I50 value of approximately 10 microM. The removal of these inhibitors from the culture medium increased the reduced mitotic activity reversibly. Using a fluorescent assay of DNA binding of ethidium bromide, no dead cells were detected in any of the cultures. These results indicate that the inhibitory effects of SK&F 96365 and DBHQ on cell proliferation were due to the inhibition of capacitative Ca2+ entry and Ca2+ mobilization suggesting the importance of capacitative Ca2+ entry and Ca2+ mobilization in the control of EGF-induced cell cycle progression in mouse mammary epithelial cells.

  12. HIV Life Cycle

    Science.gov (United States)

    HIV Overview The HIV Life Cycle (Last updated 9/13/2016; last reviewed 9/8/2016) Key Points HIV gradually destroys the immune ... life cycle. What is the connection between the HIV life cycle and HIV medicines? Antiretroviral therapy (ART) ...

  13. Cycling To Awareness.

    Science.gov (United States)

    Kozak, Stan

    1999-01-01

    Encourages environmental and outdoor educators to promote bicycling. In the community and the curriculum, cycling connects environmental issues, health and fitness, law and citizenship, appropriate technology, and the joy of being outdoors. Describes the Ontario Cycling Association's cycling strategy and its four components: school cycling…

  14. Hereditary urea cycle abnormality

    Science.gov (United States)

    ... vitro so the specific genetic cause is known. Teamwork between parents, the affected child, and doctors can help prevent severe illness. Alternative Names Abnormality of the urea cycle - hereditary; Urea cycle - hereditary abnormality Images Male urinary system Urea cycle References Lichter-Konecki ...

  15. Adaptation of a malaria surveillance system for use in a visceral leishmaniasis elimination programme.

    Science.gov (United States)

    Foster, Geraldine M; Dunkley, Sophie; Deb, Rinki M; Thomsen, Edward; Coleman, Marlize; Dhariwal, A C; Das Gupta, R K; Srikantiah, Sridhar; Das, Pradeep; Coleman, Michael

    2017-05-01

    Successful public practice relies on generation and use of high-quality data. A data surveillance system (the Disease Data Management System [DDMS]) in use for malaria was adapted for use in the Indian visceral leishmaniasis elimination programme. A situational analysis identified the data flows in current use. Taxonomic trees for the vector of visceral leishmaniasis in India, Phlebotomus argentipes, were incorporated into the DDMS to allow entry of quality assurance and insecticide susceptibility data. A new quality assurance module was created to collate the concentration of DDT that was applied to walls during the indoor residual spraying (IRS) vector control programme. The DDMS was implemented in Bihar State and used to collate and manage data from sentinel sites in eight districts. Quality assurance data showed that DDT was under-applied to walls during IRS; this, combined with insecticide susceptibility data showing widespread vector resistance to DDT prompted a national policy change to using compression pumps and alpha-cypermethrin insecticide for IRS. The adapted DDMS centralises programmatic data and enhances evidence-based decision making and active policy change. Moving forward, further modules of the system will be implemented, allowing extended data capture and streamlined transmission of key information to decision makers.

  16. Elimination of self-reactive T cells in the thymus: a timeline for negative selection.

    Directory of Open Access Journals (Sweden)

    Ivan Lilyanov Dzhagalov

    Full Text Available The elimination of autoreactive T cells occurs via thymocyte apoptosis and removal by thymic phagocytes, but the sequence of events in vivo, and the relationship between thymocyte death and phagocytic clearance, are unknown. Here we address these questions by following a synchronized cohort of thymocytes undergoing negative selection within a three-dimensional thymic tissue environment, from the initial encounter with a negative selecting ligand to thymocyte death and clearance. Encounter with cognate peptide-MHC complexes results in rapid calcium flux and migratory arrest in auto-reactive thymocytes over a broad range of peptide concentrations, followed by a lag period in which gene expression changes occurred, but there was little sign of thymocyte death. Caspase 3 activation and thymocyte loss were first detectable at 2 and 3 hours, respectively, and entry of individual thymocytes into the death program occurred asynchronously over the next 10 hours. Two-photon time-lapse imaging revealed that thymocyte death and phagocytosis occurred simultaneously, often with thymocytes engulfed prior to changes in chromatin and membrane permeability. Our data provide a timeline for negative selection and reveal close coupling between cell death and clearance in the thymus.

  17. Caveolin-1 associated adenovirus entry into human corneal cells.

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    Mohammad A Yousuf

    Full Text Available The cellular entry of viruses represents a critical area of study, not only for viral tropism, but also because viral entry dictates the nature of the immune response elicited upon infection. Epidemic keratoconjunctivitis (EKC, caused by viruses within human adenovirus species D (HAdV-D, is a severe, ocular surface infection associated with corneal inflammation. Clathrin-mediated endocytosis has previously been shown to play a critical role in entry of other HAdV species into many host cell types. However, HAdV-D endocytosis into corneal cells has not been extensively studied. Herein, we show an essential role for cholesterol rich, lipid raft microdomains and caveolin-1, in the entry of HAdV-D37 into primary human corneal fibroblasts. Cholesterol depletion using methyl-β-cyclodextrin (MβCD profoundly reduced viral infection. When replenished with soluble cholesterol, the effect of MβCD was reversed, allowing productive viral infection. HAdV-D37 DNA was identified in caveolin-1 rich endosomal fractions after infection. Src kinase activity was also increased in caveolin-1 rich endosomal fractions after infection, and Src phosphorylation and CXCL1 induction were both decreased in caveolin-1-/- mice corneas compared to wild type mice. siRNA knock down of caveolin-1 in corneal cells reduced chemokine induction upon viral infection, and caveolin-1-/- mouse corneas showed reduced cellular entry of HAdV-D37. As a control, HAdV-C2, a non-corneal pathogen, appeared to utilize the caveolar pathway for entry into A549 cells, but failed to infect corneal cells entirely, indicating virus and cell specific tropism. Immuno-electron microscopy confirmed the presence of caveolin-1 in HAdV-D37-containing vesicles during the earliest stages of viral entry. Collectively, these experiments indicate for the first time that HAdV-D37 uses a lipid raft mediated caveolin-1 associated pathway for entry into corneal cells, and connects the processes of viral entry with

  18. Advanced entry guidance algorithm with landing footprint computation

    Science.gov (United States)

    Leavitt, James Aaron

    The design and performance evaluation of an entry guidance algorithm for future space transportation vehicles is presented. The algorithm performs two functions: on-board trajectory planning and trajectory tracking. The planned longitudinal path is followed by tracking drag acceleration, as is done by the Space Shuttle entry guidance. Unlike the Shuttle entry guidance, lateral path curvature is also planned and followed. A new trajectory planning function for the guidance algorithm is developed that is suitable for suborbital entry and that significantly enhances the overall performance of the algorithm for both orbital and suborbital entry. In comparison with the previous trajectory planner, the new planner produces trajectories that are easier to track, especially near the upper and lower drag boundaries and for suborbital entry. The new planner accomplishes this by matching the vehicle's initial flight path angle and bank angle, and by enforcing the full three-degree-of-freedom equations of motion with control derivative limits. Insights gained from trajectory optimization results contribute to the design of the new planner, giving it near-optimal downrange and crossrange capabilities. Planned trajectories and guidance simulation results are presented that demonstrate the improved performance. Based on the new planner, a method is developed for approximating the landing footprint for entry vehicles in near real-time, as would be needed for an on-board flight management system. The boundary of the footprint is constructed from the endpoints of extreme downrange and crossrange trajectories generated by the new trajectory planner. The footprint algorithm inherently possesses many of the qualities of the new planner, including quick execution, the ability to accurately approximate the vehicle's glide capabilities, and applicability to a wide range of entry conditions. Footprints can be generated for orbital and suborbital entry conditions using a pre

  19. Predictor-corrector entry guidance with waypoint and no-fly zone constraints

    Science.gov (United States)

    Wang, Tao; Zhang, Hongbo; Tang, Guojian

    2017-09-01

    With the requirement of the future entry mission, the guidance method not only needs to be robust, reliable and autonomous, but also needs to have the ability to meet the constraints of no-fly zone and waypoint. The paper proposes a predictor-corrector entry guidance method that satisfies the constraints of no-fly zone and waypoint. The trajectory prediction is realized by numerical integration and the corrector is based on fuzzy logic. No-fly zones and waypoints are transformed to a series of reference points. For each reference point, one bank angle reversal is designed. The attack angle, the magnitude and reversal times of bank angle are cooperatively corrected to meet all constraints. The proposed method can guide longitudinal motion and lateral motion synergistically, so it is more robust and flexible. Only one bank angle reversal is needed for a no-fly zone or waypoint, so the reversal times are few. Moreover, without iteration, only a single trajectory prediction is required in a correction cycle, which is favorable for on-board calculation.

  20. Dopamine Receptor Activation Increases HIV Entry into Primary Human Macrophages

    Science.gov (United States)

    Gaskill, Peter J.; Yano, Hideaki H.; Kalpana, Ganjam V.; Javitch, Jonathan A.; Berman, Joan W.

    2014-01-01

    Macrophages are the primary cell type infected with HIV in the central nervous system, and infection of these cells is a major component in the development of neuropathogenesis and HIV-associated neurocognitive disorders. Within the brains of drug abusers, macrophages are exposed to increased levels of dopamine, a neurotransmitter that mediates the addictive and reinforcing effects of drugs of abuse such as cocaine and methamphetamine. In this study we examined the effects of dopamine on HIV entry into primary human macrophages. Exposure to dopamine during infection increased the entry of R5 tropic HIV into macrophages, irrespective of the concentration of the viral inoculum. The entry pathway affected was CCR5 dependent, as antagonizing CCR5 with the small molecule inhibitor TAK779 completely blocked entry. The effect was dose-dependent and had a steep threshold, only occurring above 108 M dopamine. The dopamine-mediated increase in entry required dopamine receptor activation, as it was abrogated by the pan-dopamine receptor antagonist flupenthixol, and could be mediated through both subtypes of dopamine receptors. These findings indicate that the effects of dopamine on macrophages may have a significant impact on HIV pathogenesis. They also suggest that drug-induced increases in CNS dopamine may be a common mechanism by which drugs of abuse with distinct modes of action exacerbate neuroinflammation and contribute to HIV-associated neurocognitive disorders in infected drug abusers. PMID:25268786

  1. Dopamine receptor activation increases HIV entry into primary human macrophages.

    Directory of Open Access Journals (Sweden)

    Peter J Gaskill

    Full Text Available Macrophages are the primary cell type infected with HIV in the central nervous system, and infection of these cells is a major component in the development of neuropathogenesis and HIV-associated neurocognitive disorders. Within the brains of drug abusers, macrophages are exposed to increased levels of dopamine, a neurotransmitter that mediates the addictive and reinforcing effects of drugs of abuse such as cocaine and methamphetamine. In this study we examined the effects of dopamine on HIV entry into primary human macrophages. Exposure to dopamine during infection increased the entry of R5 tropic HIV into macrophages, irrespective of the concentration of the viral inoculum. The entry pathway affected was CCR5 dependent, as antagonizing CCR5 with the small molecule inhibitor TAK779 completely blocked entry. The effect was dose-dependent and had a steep threshold, only occurring above 108 M dopamine. The dopamine-mediated increase in entry required dopamine receptor activation, as it was abrogated by the pan-dopamine receptor antagonist flupenthixol, and could be mediated through both subtypes of dopamine receptors. These findings indicate that the effects of dopamine on macrophages may have a significant impact on HIV pathogenesis. They also suggest that drug-induced increases in CNS dopamine may be a common mechanism by which drugs of abuse with distinct modes of action exacerbate neuroinflammation and contribute to HIV-associated neurocognitive disorders in infected drug abusers.

  2. A Novel Keyless Entry System Using Visible Light Communication

    Directory of Open Access Journals (Sweden)

    Kuniyoshi Okuda

    2014-10-01

    Full Text Available A major conventional car key is the mechanical key. When users inserted the key into a cylinder mechanical key and turn, users can unlock. The mechanical key may be able to be worn away for physical contact. And it may be impossible to use. Further the mechanical key may be damaged or destroyed by mischief. So a keyless entry system has been developed. The keyless entry system locks and unlocks using the infrared ray and radio waves communication. The keyless entry system does not require physical contacts. Therefore, the possibilities of damage due to mischief and be worn away are fell. However, the transmission ranges of infrared ray and radio waves communication are not clear. Submitting data might be tapped to the malicious people. If the information is tapped, then you have theft of the car and the risk of car break. We propose a keyless entry system using visible light communication in order to solve this problem. Visible light communication transmits signal using blinking light. Thus the transmission range is clear. The user can transmit the information only to the aimed place. Therefore, the usability is improved. We measure the durations of "take out the remote control", "put the aim" and "takes to unlock key" in order to evaluate the usability in the experiment. We also compared with the infrared ray communication and examined the superiority. Usability is improved in the experimental results, and usability is better than conventional keyless entry system.

  3. Structural and mechanistic studies of measles virus illuminate paramyxovirus entry.

    Directory of Open Access Journals (Sweden)

    Richard K Plemper

    2011-06-01

    Full Text Available Measles virus (MeV, a member of the paramyxovirus family of enveloped RNA viruses and one of the most infectious viral pathogens identified, accounts for major pediatric morbidity and mortality worldwide although coordinated efforts to achieve global measles control are in place. Target cell entry is mediated by two viral envelope glycoproteins, the attachment (H and fusion (F proteins, which form a complex that achieves merger of the envelope with target cell membranes. Despite continually expanding knowledge of the entry strategies employed by enveloped viruses, our molecular insight into the organization of functional paramyxovirus fusion complexes and the mechanisms by which the receptor binding by the attachment protein triggers the required conformational rearrangements of the fusion protein remain incomplete. Recently reported crystal structures of the MeV attachment protein in complex with its cellular receptors CD46 or SLAM and newly developed functional assays have now illuminated some of the fundamental principles that govern cell entry by this archetype member of the paramyxovirus family. Here, we review these advances in our molecular understanding of MeV entry in the context of diverse entry strategies employed by other members of the paramyxovirus family.

  4. A Gestalt approach to Gram-negative entry.

    Science.gov (United States)

    Silver, Lynn L

    2016-12-15

    A major obstacle confronting the discovery and development of new antibacterial agents to combat resistant Gram-negative (GN) organisms is the lack of a rational process for endowing compounds with properties that allow (or promote) entry into the bacterial cytoplasm. The major permeability difference between GN and Gram-positive (GP) bacteria is the GN outer membrane (OM) which is a permeability barrier itself and potentiates efflux pumps that expel compounds. Based on the fact that OM-permeable and efflux-deleted GNs are sensitive to many anti-GP drugs, recent efforts to approach the GN entry problem have focused on ways of avoiding efflux and transiting or compromising the OM, with the tacit assumption that this could allow entry of compounds into the GN cytoplasm. But bypassing the OM and efflux obstacles does not take into account the additional requirement of penetrating the cytoplasmic membrane (CM) whose sieving properties appear to be orthogonal to that of the OM. That is, tailoring compounds to transit the OM may well compromise their ability to enter the cytoplasm. Thus, a Gestalt approach to understanding the chemical requirements for GN entry seems a useful adjunct. This might consist of characterizing compounds which reach the cytoplasm, grouping (or binning) by routes of entry and formulating chemical 'rules' for those bins. This will require acquisition of data on large numbers of compounds, using non-activity-dependent methods of measuring accumulation in the cytoplasm. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Trading Robustness Requirements in Mars Entry Trajectory Design

    Science.gov (United States)

    Lafleur, Jarret M.

    2009-01-01

    One of the most important metrics characterizing an atmospheric entry trajectory in preliminary design is the size of its predicted landing ellipse. Often, requirements for this ellipse are set early in design and significantly influence both the expected scientific return from a particular mission and the cost of development. Requirements typically specify a certain probability level (6-level) for the prescribed ellipse, and frequently this latter requirement is taken at 36. However, searches for the justification of 36 as a robustness requirement suggest it is an empirical rule of thumb borrowed from non-aerospace fields. This paper presents an investigation into the sensitivity of trajectory performance to varying robustness (6-level) requirements. The treatment of robustness as a distinct objective is discussed, and an analysis framework is presented involving the manipulation of design variables to effect trades between performance and robustness objectives. The scenario for which this method is illustrated is the ballistic entry of an MSL-class Mars entry vehicle. Here, the design variable is entry flight path angle, and objectives are parachute deploy altitude performance and error ellipse robustness. Resulting plots show the sensitivities between these objectives and trends in the entry flight path angles required to design to these objectives. Relevance to the trajectory designer is discussed, as are potential steps for further development and use of this type of analysis.

  6. BST2/Tetherin enhances entry of human cytomegalovirus.

    Directory of Open Access Journals (Sweden)

    Kasinath Viswanathan

    2011-11-01

    Full Text Available Interferon-induced BST2/Tetherin prevents budding of vpu-deficient HIV-1 by tethering mature viral particles to the plasma membrane. BST2 also inhibits release of other enveloped viruses including Ebola virus and Kaposi's sarcoma associated herpesvirus (KSHV, indicating that BST2 is a broadly acting antiviral host protein. Unexpectedly however, recovery of human cytomegalovirus (HCMV from supernatants of BST2-expressing human fibroblasts was increased rather than decreased. Furthermore, BST2 seemed to enhance viral entry into cells since more virion proteins were released into BST2-expressing cells and subsequent viral gene expression was elevated. A significant increase in viral entry was also observed upon induction of endogenous BST2 during differentiation of the pro-monocytic cell line THP-1. Moreover, treatment of primary human monocytes with siRNA to BST2 reduced HCMV infection, suggesting that BST2 facilitates entry of HCMV into cells expressing high levels of BST2 either constitutively or in response to exogenous stimuli. Since BST2 is present in HCMV particles we propose that HCMV entry is enhanced via a reverse-tethering mechanism with BST2 in the viral envelope interacting with BST2 in the target cell membrane. Our data suggest that HCMV not only counteracts the well-established function of BST2 as inhibitor of viral egress but also employs this anti-viral protein to gain entry into BST2-expressing hematopoietic cells, a process that might play a role in hematogenous dissemination of HCMV.

  7. Rhadinovirus host entry by co-operative infection.

    Directory of Open Access Journals (Sweden)

    Clara Lawler

    2015-03-01

    Full Text Available Rhadinoviruses establish chronic infections of clinical and economic importance. Several show respiratory transmission and cause lung pathologies. We used Murid Herpesvirus-4 (MuHV-4 to understand how rhadinovirus lung infection might work. A primary epithelial or B cell infection often is assumed. MuHV-4 targeted instead alveolar macrophages, and their depletion reduced markedly host entry. While host entry was efficient, alveolar macrophages lacked heparan - an important rhadinovirus binding target - and were infected poorly ex vivo. In situ analysis revealed that virions bound initially not to macrophages but to heparan⁺ type 1 alveolar epithelial cells (AECs. Although epithelial cell lines endocytose MuHV-4 readily in vitro, AECs did not. Rather bound virions were acquired by macrophages; epithelial infection occurred only later. Thus, host entry was co-operative - virion binding to epithelial cells licensed macrophage infection, and this in turn licensed AEC infection. An antibody block of epithelial cell binding failed to block host entry: opsonization provided merely another route to macrophages. By contrast an antibody block of membrane fusion was effective. Therefore co-operative infection extended viral tropism beyond the normal paradigm of a target cell infected readily in vitro; and macrophage involvement in host entry required neutralization to act down-stream of cell binding.

  8. Progress Toward Regional Measles Elimination - Worldwide, 2000-2015.

    Science.gov (United States)

    Patel, Minal K; Gacic-Dobo, Marta; Strebel, Peter M; Dabbagh, Alya; Mulders, Mick N; Okwo-Bele, Jean-Marie; Dumolard, Laure; Rota, Paul A; Kretsinger, Katrina; Goodson, James L

    2016-11-11

    Adopted in 2000, United Nations Millennium Development Goal 4 set a target to reduce child mortality by two thirds by 2015, with measles vaccination coverage as one of the progress indicators. In 2010, the World Health Assembly (WHA) set three milestones for measles control by 2015: 1) increase routine coverage with the first dose of measles-containing vaccine (MCV1) for children aged 1 year to ≥90% nationally and ≥80% in every district; 2) reduce global annual measles incidence to WHA endorsed the Global Vaccine Action Plan(†) with the objective to eliminate measles in four World Health Organization (WHO) regions by 2015. Countries in all six WHO regions have adopted measles elimination goals. Measles elimination is the absence of endemic measles transmission in a region or other defined geographical area for ≥12 months in the presence of a well performing surveillance system. This report updates a previous report (3) and describes progress toward global measles control milestones and regional measles elimination goals during 2000-2015. During this period, annual reported measles incidence decreased 75%, from 146 to 36 cases per 1 million persons, and annual estimated measles deaths decreased 79%, from 651,600 to 134,200. However, none of the 2015 milestones or elimination goals were met. Countries and their partners need to act urgently to secure political commitment, raise the visibility of measles, increase vaccination coverage, strengthen surveillance, and mitigate the threat of decreasing resources for immunization once polio eradication is achieved.

  9. Progress toward regional measles elimination--worldwide, 2000-2013.

    Science.gov (United States)

    Perry, Robert T; Gacic-Dobo, Marta; Dabbagh, Alya; Mulders, Mick N; Strebel, Peter M; Okwo-Bele, Jean-Marie; Rota, Paul A; Goodson, James L

    2014-11-14

    In 2012, the World Health Assembly endorsed the Global Vaccine Action Plan with the objective to eliminate measles in four World Health Organization (WHO) regions by 2015. Member states of all six WHO regions have adopted measles elimination goals. In 2010, the World Health Assembly established three milestones for 2015: 1) increase routine coverage with the first dose of measles-containing vaccine (MCV1) for children aged 1 year to ≥90% nationally and ≥80% in every district; 2) reduce global annual measles incidence to measles mortality by 95% from the 2000 estimate. This report updates the 2000-2012 report and describes progress toward global control and regional measles elimination during 2000-2013. During this period, annual reported measles incidence declined 72% worldwide, from 146 to 40 per million population, and annual estimated measles deaths declined 75%, from 544,200 to 145,700. Four of six WHO regions have established regional verification commissions (RVCs); in the European (EUR) and Western Pacific regions (WPR), 19 member states successfully documented the absence of endemic measles. Resuming progress toward 2015 milestones and elimination goals will require countries and their partners to raise the visibility of measles elimination, address barriers to measles vaccination, and make substantial and sustained additional investments in strengthening health systems.

  10. TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.

    Directory of Open Access Journals (Sweden)

    Pradeep D Uchil

    2008-02-01

    Full Text Available Members of the TRIpartite interaction Motif (TRIM family of E3 ligases have been shown to exhibit antiviral activities. Here we report a near comprehensive screen for antiretroviral activities of 55 TRIM proteins (36 human, 19 mouse. We identified approximately 20 TRIM proteins that, when transiently expressed in HEK293 cells, affect the entry or release of human immunodeficiency virus 1 (HIV, murine leukemia virus (MLV, or avian leukosis virus (ALV. While TRIM11 and 31 inhibited HIV entry, TRIM11 enhanced N-MLV entry by interfering with Ref1 restriction. Strikingly, many TRIM proteins affected late stages of the viral life cycle. Gene silencing of endogenously expressed TRIM 25, 31, and 62 inhibited viral release indicating that they play an important role at late stages of the viral life cycle. In contrast, downregulation of TRIM11 and 15 enhanced virus release suggesting that these proteins contribute to the endogenous restriction of retroviruses in cells.

  11. APC/C and SCF(cyclin F) Constitute a Reciprocal Feedback Circuit Controlling S-Phase Entry.

    Science.gov (United States)

    Choudhury, Rajarshi; Bonacci, Thomas; Arceci, Anthony; Lahiri, Debojyoti; Mills, Christine A; Kernan, Jennifer L; Branigan, Timothy B; DeCaprio, James A; Burke, Daniel J; Emanuele, Michael J

    2016-09-20

    The anaphase promoting complex/cyclosome (APC/C) is an ubiquitin ligase and core component of the cell-cycle oscillator. During G1 phase, APC/C binds to its substrate receptor Cdh1 and APC/C(Cdh1) plays an important role in restricting S-phase entry and maintaining genome integrity. We describe a reciprocal feedback circuit between APC/C and a second ubiquitin ligase, the SCF (Skp1-Cul1-F box). We show that cyclin F, a cell-cycle-regulated substrate receptor (F-box protein) for the SCF, is targeted for degradation by APC/C. Furthermore, we establish that Cdh1 is itself a substrate of SCF(cyclin F). Cyclin F loss impairs Cdh1 degradation and delays S-phase entry, and this delay is reversed by simultaneous removal of Cdh1. These data indicate that the coordinated, temporal ordering of cyclin F and Cdh1 degradation, organized in a double-negative feedback loop, represents a fundamental aspect of cell-cycle control. This mutual antagonism could be a feature of other oscillating systems.

  12. Graduate entry to medicine: widening psychological diversity

    Directory of Open Access Journals (Sweden)

    Munro Don

    2009-11-01

    Full Text Available Abstract Background At Nottingham University more than 95% of entrants to the traditional 5-year medical course are school leavers. Since 2003 we have admitted graduate entrants (GEM to a shortened (4-year course to 'widen access to students from more disadvantaged backgrounds'. We have recently shown that the GEM course widens academic and socio-demographic diversity of the medical student population. This study explored whether GEM students also bring psychological diversity and whether this could be beneficial. Methods We studied: a 217 and 96 applicants to the Nottingham 5- and 4-year courses respectively, applying in the 2002-3 UCAS cycle, and, b 246 school leavers starting the 5-year course and 39 graduate entrants to the 4-year course in October 2003. The psychological profiles of the two groups of applicants and two groups of entrants were compared using their performance in the Goldberg 'Big 5' Personality test, the Personal Qualities Assessment (PQA; measuring interpersonal traits and interpersonal values, and the Lovibond and Lovibond measure of depression, anxiety and stress. For the comparison of the Entrants we excluded the 33 school leavers and seven graduates who took the tests as Applicants. Statistical analyses were undertaken using SPSS software (version 16.0. Results Graduate applicants compared to school leaver applicants were significantly more conscientious, more confident, more self controlled, more communitarian in moral orientation and less anxious. Only one of these differences was preserved in the entrants with graduates being less anxious. However, the graduate entrants were significantly less empathetic and conscientious than the school leavers. Conclusion This study has shown that school leaver and graduate entrants to medical school differ in some psychological characteristics. However, if confirmed in other studies and if they were manifest in the extreme, not all the traits brought by graduates would be

  13. Eliminating the Neglected Tropical Diseases: Translational Science and New Technologies.

    Directory of Open Access Journals (Sweden)

    Peter J Hotez

    2016-03-01

    Full Text Available Today, the World Health Organization recognizes 17 major parasitic and related infections as the neglected tropical diseases (NTDs. Despite recent gains in the understanding of the nature and prevalence of NTDs, as well as successes in recent scaled-up preventive chemotherapy strategies and other health interventions, the NTDs continue to rank among the world's greatest global health problems. For virtually all of the NTDs (including those slated for elimination under the auspices of a 2012 London Declaration for NTDs and a 2013 World Health Assembly resolution [WHA 66.12], additional control mechanisms and tools are needed, including new NTD drugs, vaccines, diagnostics, and vector control agents and strategies. Elimination will not be possible without these new tools. Here we summarize some of the key challenges in translational science to develop and introduce these new technologies in order to ensure success in global NTD elimination efforts.

  14. Route Elimination Heuristic for Vehicle Routing Problem with Time Windows

    Directory of Open Access Journals (Sweden)

    Sándor Csiszár

    2005-11-01

    Full Text Available The paper deals with the design of a route elimination (RE algorithm for thevehicle routing problem with time windows (VRPTW. The problem has two objectives, oneof them is the minimal number of routes the other is the minimal cost. To cope with theseobjectives effectively two-phase solutions are often suggested in the relevant literature. Inthe first phase the main focus is the route elimination, in the second one it is the costreduction. The algorithm described here is a part of a complete VRPWT study. The methodwas developed by studying the graph behaviour during the route elimination. For thispurpose a model -called “Magic Bricks” was developed. The computation results on theSolomon problem set show that the developed algorithm is competitive with the best ones.

  15. Fast control latency uncertainty elimination for the BESIII ETOF upgrade

    Science.gov (United States)

    Wang, Yun; Cao, Ping; Liu, Shu-bin; An, Qi

    2016-09-01

    A new fanning topology is proposed to precisely fan out fast control signals in the Beijing Spectrometer (BESIII) end-cap time-of-flight (ETOF) electronics. However, uncertainty in transfer latency is introduced by the new fanning channel, which will degrade the precision of fast control. In this paper, latency uncertainty elimination for the BESIII ETOF upgrade is introduced. The latency uncertainty is determined by a Time-Digital-Converter (TDC) embedded in a Field-Programmable Gate Array (FPGA) and is eliminated by re-capturing at synchronous and determinate time. Compared with the existing method of Barrel-cap TOF (BTOF), it has advantages of flexible structure, easy calibration and good adaptability. Field tests on the BESIII ETOF system show that this method effectively eliminates transfer latency uncertainty. Supported by CAS Maintenance Project for Major Scientific and Technological Infrastructure (IHEP-SW-953/2013)

  16. Removal of Quantifiers by Elimination of Boundary Points

    CERN Document Server

    Goldberg, Eugene

    2012-01-01

    We consider the problem of elimination of existential quantifiers from a Boolean CNF formula. Our approach is based on the following observation. One can get rid of dependency on a set of variables of a quantified CNF formula F by adding resolvent clauses of F eliminating boundary points. This approach is similar to the method of quantifier elimination described in [9]. The difference of the method described in the present paper is twofold: {\\bullet} branching is performed only on quantified variables, {\\bullet} an explicit search for boundary points is performed by calls to a SAT-solver Although we published the paper [9] before this one, chrono- logically the method of the present report was developed first. Preliminary presentations of this method were made in [10], [11]. We postponed a publication of this method due to preparation of a patent application [8].

  17. Passive Elimination of Static Electricity in Oil Industry

    Directory of Open Access Journals (Sweden)

    Mićo Gaćanović

    2015-04-01

    Full Text Available This study explains the existing and real conditions of a possible passive elimination of static electricity when loading oil and oil derivatives. We are considering the formation and survival of gas bubbles both in the volume of oil in its depth, but also at the surface of oil and oil derivatives of the partly filled reservoir, and formation of both volume and surface electric charge in oil and oil derivatives. The study presents the research of formation and survival of static electricity in both reservoirs and tank trucks of different geometric shapes partly filled with oil and oil derivatives. We are proposing a new original possibility of passive elimination of static electricity when loading oil and oil derivatives in reservoirs and tank trucks. The proposed passive device for elimination of static electricity is protected at the international level in the domain of intellectual property (with a patent, model and distinctive mark.

  18. Passive elimination of static electricity in oil industry

    Directory of Open Access Journals (Sweden)

    Gaćanović Mićo

    2014-01-01

    Full Text Available This study explains the existing and real conditions of a possible passive elimination of static electricity when loading oil and oil derivatives. We are considering the formation and survival of gas bubbles both in the volume of oil in its depth, but also at the surface of oil and oil derivatives of the partly filled reservoir, and formation of both volume and surface electric charge in oil and oil derivatives. The study presents the research of formation and survival of static electricity in both reservoirs and tank trucks of different geometric shapes partly filled with oil and oil derivatives. We are proposing a new original possibility of passive elimination of static electricity when loading oil and oil derivatives in reservoirs and tank trucks. The proposed passive device for elimination of static electricity is protected at the international level in the domain of intellectual property (with a patent, model and distinctive mark.

  19. Risk Management - Variance Minimization or Lower Tail Outcome Elimination

    DEFF Research Database (Denmark)

    Aabo, Tom

    2002-01-01

    This paper illustrates the profound difference between a risk management strategy of variance minimization and a risk management strategy of lower tail outcome elimination. Risk managers concerned about the variability of cash flows will tend to center their hedge decisions on their best guess...... on future cash flows (the budget), while risk managers concerned about costly lower tail outcomes will hedge (considerably) less depending on the level of uncertainty. A risk management strategy of lower tail outcome elimination is in line with theoretical recommendations in a corporate value......-adding perspective. A cross-case study of blue-chip industrial companies partly supports the empirical use of a risk management strategy of lower tail outcome elimination but does not exclude other factors from (co-)driving the observations....

  20. Space Shuttle Orbiter entry guidance and control system sensitivity analysis

    Science.gov (United States)

    Stone, H. W.; Powell, R. W.

    1976-01-01

    An approach has been developed to determine the guidance and control system sensitivity to off-nominal aerodynamics for the Space Shuttle Orbiter during entry. This approach, which uses a nonlinear six-degree-of-freedom interactive, digital simulation, has been applied to both the longitudinal and lateral-directional axes for a portion of the orbiter entry. Boundary values for each of the aerodynamic parameters have been identified, the key parameters have been determined, and system modifications that will increase system tolerance to off-nominal aerodynamics have been recommended. The simulations were judged by specified criteria and the performance was evaluated by use of key dependent variables. The analysis is now being expanded to include the latest shuttle guidance and control systems throughout the entry speed range.