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Sample records for culturally produced meanings

  1. Cultural Meanings Construction: An Analysis of the Organic Grape Juice Market

    Directory of Open Access Journals (Sweden)

    Marlon Dalmoro

    2015-04-01

    Full Text Available Regarding the meanings networks that involve markets, this research aims to analyze cultural meanings production in the organic grape juice market, involving consumers and producers agents. It was adopted a qualitative approach with an interpretative character by interviews with 25 consumers and producers. Cultural meanings construction description and analysis involved the socio-cultural context, interaction between agents (producers and consumers and meanings assigned by each agent. Organics are meant as food for both agents. However, others meanings associated to organics operate in dichotomic levels. It results in a non-homogeneous network between producers and consumers, reflecting the different cultural universe of each agent. These results mainly contribute in understanding the patterns of production and consumption around the food market. The lack of consolidated networks of meanings limit the construction and establishment of organic products market structures.

  2. Music, Meaning and Culture

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    Richard Widdess

    2012-09-01

    Full Text Available This paper situates musical meaning in culture, addressing music as social symbol and as ongoing process of meaning creation. Three examples of non- Western musical practice are used to illustrate the embedding of musical meaning in cultural context. The performance of an Australian Aboriginal song is shown to exemplify the interdependence of song style and social structure as a matrix for the emergence of cultural meanings; an example of North Indian performance is adduced to demonstrate the multi-layered nature of meaning as embodied in musical performance; and an example of collective festival performance from Nepal illustrates ways in which the structure of musical performance can mirror local cultural forms. Each of the three examples lends weight to the idea that music's meanings are often non-linguistic and reflect foundational schemas that are specific to the cultures from the musics are drawn.

  3. Culture as meaning-making

    OpenAIRE

    Lenkauskienė, Rūta; Liubinienė, Vilmantė

    2002-01-01

    The present paper analyses the role of social and cultural background knowledge in the cognition of meaning. Language and culture integrated studies have long been in the focus of attention. In order to study the language of a target culture, one should understand how human beings construct meanings, understand processes of meaning-making, account for different meanings, and examine their effects in social life. The language cannot be interpreted in the right way without taking the target cul...

  4. School Meaning Systems: The Symbiotic Nature of Culture and "Language-In-Use"

    Science.gov (United States)

    Abawi, Lindy

    2013-01-01

    Recent research has produced evidence to suggest a strong reciprocal link between school context-specific language constructions that reflect a school's vision and schoolwide pedagogy, and the way that meaning making occurs, and a school's culture is characterized. This research was conducted within three diverse settings: one school in the Sydney…

  5. Culture cultivating culture: the four products of the meaning-made world.

    Science.gov (United States)

    Carriere, Kevin R

    2014-09-01

    Culture, in a semiotic cultural psychology, is defined from the viewpoint of cultivation--the meaning making processes that give meaning to the world (Valsiner 2000, 2007a). However, the individual is not simply a process-machine in an empty world--there are both the external outcomes of meaning making (individual and group based) as well as the collective influence on the cultivation process. I argue to examine the cultivation process more completely, one must look at these external influences that catalyze future cultivation processes. By examining the power of the external (environmental Umwelten) and group-internal (myths, morals), a much greater understanding of the behavior of individuals can be accomplished beyond examining the individual's process of meaning making. Further work into examining the objects that affectively activate the individual as well as group action and meaning making is called for and examples of such studies are given.

  6. Greater learnability is not sufficient to produce cultural universals.

    Science.gov (United States)

    Rafferty, Anna N; Griffiths, Thomas L; Ettlinger, Marc

    2013-10-01

    Looking across human societies reveals regularities in the languages that people speak and the concepts that they use. One explanation that has been proposed for these "cultural universals" is differences in the ease with which people learn particular languages and concepts. A difference in learnability means that languages and concepts possessing a particular property are more likely to be accurately transmitted from one generation of learners to the next. Intuitively, this difference could allow languages and concepts that are more learnable to become more prevalent after multiple generations of cultural transmission. If this is the case, the prevalence of languages and concepts with particular properties can be explained simply by demonstrating empirically that they are more learnable. We evaluate this argument using mathematical analysis and behavioral experiments. Specifically, we provide two counter-examples that show how greater learnability need not result in a property becoming prevalent. First, more learnable languages and concepts can nonetheless be less likely to be produced spontaneously as a result of transmission failures. We simulated cultural transmission in the laboratory to show that this can occur for memory of distinctive items: these items are more likely to be remembered, but not generated spontaneously once they have been forgotten. Second, when there are many languages or concepts that lack the more learnable property, sheer numbers can swamp the benefit produced by greater learnability. We demonstrate this using a second series of experiments involving artificial language learning. Both of these counter-examples show that simply finding a learnability bias experimentally is not sufficient to explain why a particular property is prevalent in the languages or concepts used in human societies: explanations for cultural universals based on cultural transmission need to consider the full set of hypotheses a learner could entertain and all of

  7. The meaning of work and cultural psychology: Ideas for new directions

    DEFF Research Database (Denmark)

    Bendassolli, Pedro; Tateo, Luca

    2018-01-01

    Work is one arena in which human beings constitute their identities and participate in collective-cultural enterprises. But research on factors affecting the meaning of work and its outcomes focuses mostly on individual-level variables related to workers’ experience. However, scholars have recently...... proposed a shift towards a more collective dimension of meaningfulness, in particular, the cultural level. This article discusses and expands on this recent trend, demonstrating how growing attention to cultural factors of work’s meaning raises some problematic, crucial issues about the very definition...... of culture and its role in meaning-making. A particular issue is the assumption that culture is transmitted to people, that it is primarily a collective endeavour based on shared values and that culture can endow work with meaning. Based on a cultural psychology perspective, we revisit both the relationship...

  8. African-American Vernacular Dance: Core Culture and Meaning Operatives.

    Science.gov (United States)

    Hazzard-Gordon, Katrina

    1985-01-01

    Discusses the role of social dancing in the lives and culture of working class Black Americans. Focuses specifically on four aspects of its meaning: identity (self-esteem), cultural integrity, ingroup-outgroup, and political resistance. Bases argument on sociological, biographical, and fictional works by and about Black culture. (KH)

  9. The Effect of Starter Culture Producing Exopolysaccharide on Physicochemical Properties of Yoghurt

    Directory of Open Access Journals (Sweden)

    Anatoli Cartasev

    2017-12-01

    Full Text Available The purpose of this research was to investigate the impact of indigenous starter culture capable to synthesize exopolysaccharides (EPSs on physicochemical properties of yoghurt. Two starter cultures, EPS-producing and non-EPS-producing, were developed from the autochthonous lactic acid bacteria strains by pairwise combining Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus strains. In the present study the ropy strain of Streptococcus thermophilus CNMN LB-50 was incorporated in EPS-producing starter culture. The microstructure, viscosity, EPS amount, structural properties and syneresis of yoghurt samples were assessed. It has been established that the EPS-producing starter culture provided a reduction of structural degradation and increased degree of structural recovery after deformation. Besides, it was observed that the use of EPS synthesized starter culture in yoghurt production restrains the syneresis of the gel.

  10. Musics, Cultures and Meanings: Music as Communication

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    Ian Cross

    2012-09-01

    Full Text Available This commentary explores interpretations of concepts that lie at the focus of Richard Widdess's paper—"music", and "culture"—with the aim of specifying frameworks within which issues of musical meaning can fruitfully be addressed.

  11. Integrating cultural community psychology: activity settings and the shared meanings of intersubjectivity.

    Science.gov (United States)

    O'Donnell, Clifford R; Tharp, Roland G

    2012-03-01

    Cultural and community psychology share a common emphasis on context, yet their leading journals rarely cite each other's articles. Greater integration of the concepts of culture and community within and across their disciplines would enrich and facilitate the viability of cultural community psychology. The contextual theory of activity settings is proposed as one means to integrate the concepts of culture and community in cultural community psychology. Through shared activities, participants develop common experiences that affect their psychological being, including their cognitions, emotions, and behavioral development. The psychological result of these experiences is intersubjectivity. Culture is defined as the shared meanings that people develop through their common historic, linguistic, social, economic, and political experiences. The shared meanings of culture arise through the intersubjectivity developed in activity settings. Cultural community psychology presents formidable epistemological challenges, but overcoming these challenges could contribute to the transformation and advancement of community psychology.

  12. High-Level Culturability of Epiphytic Bacteria and Frequency of Biosurfactant Producers on Leaves

    Science.gov (United States)

    Burch, Adrien Y.; Do, Paulina T.; Sbodio, Adrian; Suslow, Trevor V.

    2016-01-01

    ABSTRACT To better characterize the bacterial community members capable of biosurfactant production on leaves, we distinguished culturable biosurfactant-producing bacteria from nonproducers and used community sequencing to compare the composition of these distinct cultured populations with that from DNA directly recovered from leaves. Communities on spinach, romaine, and head lettuce leaves were compared with communities from adjacent samples of soil and irrigation source water. Soil communities were poorly described by culturing, with recovery of cultured representatives from only 21% of the prevalent operational taxonomic units (OTUs) (>0.2% reads) identified. The dominant biosurfactant producers cultured from soil included bacilli and pseudomonads. In contrast, the cultured communities from leaves are highly representative of the culture-independent communities, with over 85% of the prevalent OTUs recovered. The dominant taxa of surfactant producers from leaves were pseudomonads as well as members of the infrequently studied genus Chryseobacterium. The proportions of bacteria cultured from head lettuce and romaine leaves that produce biosurfactants were directly correlated with the culture-independent proportion of pseudomonads in a given sample, whereas spinach harbored a wider diversity of biosurfactant producers. A subset of the culturable bacteria in irrigation water also became enriched on romaine leaves that were irrigated overhead. Although our study was designed to identify surfactant producers on plants, we also provide evidence that most bacteria in some habitats, such as agronomic plant surfaces, are culturable, and these communities can be readily investigated and described by more classical culturing methods. IMPORTANCE The importance of biosurfactant production to the bacteria that live on waxy leaf surfaces as well as their ability to be accurately assessed using culture-based methodologies was determined by interrogating epiphytic populations by

  13. Consumer Culture Theory: Ideology, Mythology and Meaning in Technology Consumption

    DEFF Research Database (Denmark)

    Bajde, Domen

    2014-01-01

    innovations, thus shaping the value of technologies as cultural resources sustaining consumer identities. In its urge to shed light on these aspects, CCT tends to reinforce the gaps and asymmetries between the “socio-cultural” and the “techno-material”, leaving plenty of room for further study. The authors......Consumer culture theory helps us take note of the cultural forces and dynamics in which technology consumption is entangled. It enables people to articulate the cultural processes (ideological, mythic, ritualistic, etc.) through which cultural meanings become granted to or denied to technological...

  14. Cinema as Cultural Pedagogy: Meanings by Elderly Women

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    Wânia Ribeiro Fernandes

    2010-01-01

    Full Text Available Using the theoretical reference of the cultural studies- mainly some foucauldian and feminist studies notions- this paper focuses upon the influence of films on the construction of gender identities. After a brief discussion on aspects of culture, power and identities formation, the meanings established by a group of elderly women on films they have attended during their youth are analyzed. We bring into evidence the effects that thediscourses present in that media had upon those women’s identitary construction and production.

  15. Cross-Cultural Understanding for Global Sustainability: Messages and Meanings from Asian Cultural Landscapes

    Science.gov (United States)

    Singh, R.

    2013-11-01

    Concept of 'multifunctionality' of cultural landscapes is a reflection of imbued meaning and aesthetics inherent there and also human manifestation of this spirit through existence and aliveness by human creation, love and continuance in various cultures and traditions. This sense helps envisioning landscapes that cross urban-rural divides in sustainable and an integrated way - characterised by wholeness and ecospirituality that developed in the cultural history of landscape sustainability. That is how, the idea of 'wholeness' (cosmality) is transformed into 'holiness' (sacrality) ― evolved and represented with sacred ecology and visualised through the cosmic frames of sacredscapes in Asian region that survived there as part of lifeworld. Understanding, feeling, living with, practicing and passing on these inherent meanings and aesthetics provide peace, solace and deeper feelings to human mind which are the ethereal breathe of sustainability. The rethinking should be based on the foundational value ― the reasoning that underlies the ethical sense of deeper understanding of Man-Nature Interrelatedness, the basic philosophy of coexistence ― referred in different cultures in their own ways, like multicultural co-living ('Old-comer') in Korea, harmonious coexistence (tabunka kyosei) in Japan, harmonious society (xiaokang) in China, wahi tapu (sacred places) in Maori's New Zealand, global family (vasudhaiva kutumbakam) in Indian thought, and also African humanism (ubuntu) in South Africa. Think universally, see globally, behave regionally, act locally but insightfully; this is an appeal for shared wisdom for global sustainability in making our cultural landscapes mosaic of happy, peaceful and sustainable places crossing all the borders and transitions, especially interwoven links among Korea, Japan, China, and India.

  16. Cultural Meanings and Consumers’ Discourses about Their Brand Abandonment

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    Filipe Diniz

    2018-03-01

    Full Text Available Although we know a lot about how brand meanings are created and perpetuated in relation to and through cultural discourses, remarkably little work has been done in the marketing field to develop a better comprehension as to how brand meanings are enacted through distancing behaviors, such as brand abandonment. In the marketing literature, abandonment has usually been associated with relationship crises, most commonly as the result of consumer dissatisfaction. This study investigates consumers who abandoned previously consumed brands in two distinct product categories, soft drinks and automobiles. Through investigation of two emblematic brands – CocaCola and Fiat - the analysis illustrates cultural discourses that consumers use to give meanings and socially negotiate their brand abandonment. Considering the repertoire of meanings attached to both the brands and consumers’ commitment to their distancing behavior, the analysis presents three types of brand abandonment: (a contingent, (b balanced and (c aversive. This paper also presents brand abandonment as an enabler of social distinctions, highlighting two discrete discourses promulgated and perpetuated by consumers: (a Life evolution, and (b Rationality, self-control and sovereignty.

  17. The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination

    Science.gov (United States)

    Mu, Yang; Yang, Hou-Yun; Wang, Ya-Zhou; He, Chuan-Shu; Zhao, Quan-Bao; Wang, Yi; Yu, Han-Qing

    2014-06-01

    Fermentative hydrogen production from wastes has many advantages compared to various chemical methods. Methodology for characterizing the hydrogen-producing activity of anaerobic mixed cultures is essential for monitoring reactor operation in fermentative hydrogen production, however there is lack of such kind of standardized methodologies. In the present study, a new index, i.e., the maximum specific hydrogen-producing activity (SHAm) of anaerobic mixed cultures, was proposed, and consequently a reliable and simple method, named SHAm test, was developed to determine it. Furthermore, the influences of various parameters on the SHAm value determination of anaerobic mixed cultures were evaluated. Additionally, this SHAm assay was tested for different types of substrates and bacterial inocula. Our results demonstrate that this novel SHAm assay was a rapid, accurate and simple methodology for determining the hydrogen-producing activity of anaerobic mixed cultures. Thus, application of this approach is beneficial to establishing a stable anaerobic hydrogen-producing system.

  18. The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination.

    Science.gov (United States)

    Mu, Yang; Yang, Hou-Yun; Wang, Ya-Zhou; He, Chuan-Shu; Zhao, Quan-Bao; Wang, Yi; Yu, Han-Qing

    2014-06-10

    Fermentative hydrogen production from wastes has many advantages compared to various chemical methods. Methodology for characterizing the hydrogen-producing activity of anaerobic mixed cultures is essential for monitoring reactor operation in fermentative hydrogen production, however there is lack of such kind of standardized methodologies. In the present study, a new index, i.e., the maximum specific hydrogen-producing activity (SHAm) of anaerobic mixed cultures, was proposed, and consequently a reliable and simple method, named SHAm test, was developed to determine it. Furthermore, the influences of various parameters on the SHAm value determination of anaerobic mixed cultures were evaluated. Additionally, this SHAm assay was tested for different types of substrates and bacterial inocula. Our results demonstrate that this novel SHAm assay was a rapid, accurate and simple methodology for determining the hydrogen-producing activity of anaerobic mixed cultures. Thus, application of this approach is beneficial to establishing a stable anaerobic hydrogen-producing system.

  19. Embodied Cultures of Mobilities

    DEFF Research Database (Denmark)

    Jensen, Ole B.

    2010-01-01

    and material artifacts. The paper target the complex relationship between the moving, sensing body and the material and built environment of infrastructures and mobility modes in order to explore what norms, and meanings, and everyday life mobility cultures are being produced and re-produced in this process....... A particular emphasis is put on how mobilities produce and re-produce norms, meanings and cultures in relation to the Everyday life perspective. By looking into walking, running, cycling, driving and mass transit mobilities different modes of embodied mobility is identified. The theoretical framework is based...... and interaction (Lynch). The argument is thus that understanding embodied cultures of mobilities from the vantage point of this paper lend is self to new interpretations, explorations and understandings of what it means to move within and between other social agents in particular material and physical...

  20. A strict anaerobic extreme thermophilic hydrogen-producing culture enriched from digested household waste

    DEFF Research Database (Denmark)

    Karakashev, Dimitar Borisov; Kotay, Shireen Meher; Trably, Eric

    2009-01-01

    The aim of this study was to enrich, characterize and identify strict anaerobic extreme thermophilic hydrogen (H-2) producers from digested household solid wastes. A strict anaerobic extreme thermophilic H-2 producing bacterial culture was enriched from a lab-scale digester treating household...... wastes at 70 degrees C. The enriched mixed culture consisted of two rod-shaped bacterial members growing at an optimal temperature of 80 degrees C and an optimal pH 8.1. The culture was able to utilize glucose, galactose, mannose, xylose, arabinose, maltose, sucrose, pyruvate and glycerol as carbon...... sources. Growth on glucose produced acetate, H-2 and carbon dioxide. Maximal H-2 production rate on glucose was 1.1 mmol l(-1) h(-1) with a maximum H-2 yield of 1.9 mole H-2 per mole glucose. 16S ribosomal DNA clone library analyses showed that the culture members were phylogenetically affiliated...

  1. THE MEANING OF HOME IN YORUBA CULTURE OKEYINKA, Y ...

    African Journals Online (AJOL)

    Osondu

    2012-10-03

    Oct 3, 2012 ... Key words: Meaning, House, Home, Yoruba culture, Cradle, Premium. Introduction ..... Amole, B., David, K. and Graham, T. (1993). The. Family House in west ... review of the Literature, The Sociological review,. Pp. 62 – 89.

  2. What Psychoanalysis, Culture And Society Mean To Me

    Science.gov (United States)

    Layton, Lynne

    2007-01-01

    The paper reviews some ways that the social and psychic have been understood in psychoanalysis and argues that a model for understanding the relation between the psychic and the social must account both for the ways that we internalize oppressive norms as well as the ways we resist them. The author proposes that we build our identities in relation to other identities circulating in our culture and that cultural hierarchies of sexism, racism, classism push us to split off part of what it means to be human, thereby creating painful individual and relational repetition compulsions. These “normative unconscious processes” replicate the unjust social norms that cause psychic pain in the first place. The paper concludes with thoughts about contemporary US culture, in which the government has abdicated responsibility toward its most vulnerable citizens and has thus rendered vulnerability and dependence shameful states. PMID:22058628

  3. When means become ends: technology producing values

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    Bjørn Hofmann

    2006-12-01

    Full Text Available Technology has become the symbol of our culture.  The claim that we are subject to a technological imperative is therefore a fundamental cultural critique: we do not control technology, rather technology controls us. An alternative way to formulate this is to claim that technology cannot be “made down” when it is made up; we just have to make the best of it. Accordingly, it has been argued that technology has evolved from being merely a means to becoming an end in itself. This article investigates this claim by analyzing the relationship between technology and values. The examples stem from the technologies of medicine and weapons because they clarify this relationship. It is argued that technology relates to values in two ways. Technology both raises general questions about values and it is value-laden due to its very function. However, although technology is value-laden, it does not necessarily give an imperative mandate. One reason for this lies in our responsibility. We are inevitably responsible for all aspects of technology, i.e. development, construction, production, commercialization, implementation, and use. Referring to a technological imperative to explain and defend our decisions with respect to technology constitutes an unjustified renunciation of our own responsibility. Hence, the article tries to underscore our responsibility by developing a technological axiology.

  4. LEXICAL MEANING AND CULTURAL ADAPTATION ON THE PRODUCT OF NESTLE DANCOW

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    I Gusti Agung Istri Aryani

    2017-10-01

    Full Text Available Abstract Commonly, an amazing and attractive advertisement intends to hypnotize their target consumer in seeing, reading, or even hearing the ads continually. Besides, the power of persuading and motivating in messages from the way of informing product benefit could also give impact to buyer in deciding to buy the product. This research discusses the contexts of advertising found on packages of two Nestle products, especially Nestle Dancow Actigo and Nestle Dancow Enrich. These products were analyzed based on pictures or symbols and text found on packages. It is aimed at finding out the context of discourse advertising on those packages. Data were collected from analysis of text, including pictures or symbols and additional questionnaires distributed to 50 respondents located at Denpasar and Gianyar. Urban society were used to evaluate concerning on their understanding of English even though Indonesian language mostly applied on packages of products. Method used in analyzing data is descriptive qualitative and quantitative with simple statistics and explanation. It showed that connotation and denotation meanings highlighted lexical items and its cultural adaptation using process of copy adaptation to fit the culture of their targeted consumers. Means found 6 words or phrases of English from 10 of them on packages understood by 50 respondents as of: 35 for Full Cream as highest score and 16 respondents for FortiGro as the lowest score. In addition, brand name, symbol and images showed producer seemed to have close relationship with consumer and able to attract target   consumer attention as majority of findings. It can be concluded that ads on packages of Nestle Dancow had succeeded to be understood by the society through the messages implied.

  5. Emotions in “the world”: cultural practices, products, and meanings of anger and shame in two individualist cultures

    Science.gov (United States)

    Boiger, Michael; Deyne, Simon De; Mesquita, Batja

    2013-01-01

    Three studies tested the idea that people’s cultural worlds are structured in ways that promote and highlight emotions and emotional responses that are beneficial in achieving central goals in their culture. Based on the idea that U.S. Americans strive for competitive individualism, while (Dutch-speaking) Belgians favor a more egalitarian variant of individualism, we predicted that anger and shame, as well as their associated responses, would be beneficial to different extents in these two cultural contexts. A questionnaire study found that cultural practices promote beneficial emotions (anger in the United States, shame in Belgium) and avoid harmful emotions (shame in the United States): emotional interactions were perceived to occur more or less frequently to the extent that they elicited culturally beneficial or harmful emotions. Similarly, a cultural product analysis showed that popular children’s books from the United States and Belgium tend to portray culturally beneficial emotions more than culturally harmful emotions. Finally, a word-association study of the shared cultural meanings surrounding anger and shame provided commensurate evidence at the level of the associated response. In each language network, anger and shame were imbued with meanings that reflected the cultural significance of the emotion: while culturally consistent emotions carried relatively stronger connotations of emotional yielding (e.g., giving in to anger and aggressing against the offender in the United States), culturally inconsistent emotions carried relatively stronger connotations of emotional containment (e.g., a stronger emphasis on suppressing or transforming shame in the United States). PMID:24367340

  6. How Do Cultural Producers Make Creative Decisions? Lessons from the Catwalk

    Science.gov (United States)

    Godart, Frederic C. Mears, Ashley

    2009-01-01

    Faced with high uncertainty, how do producers in the cultural economy make creative decisions? We present a case study of the fashion modeling industry. Using participant observation, interviews and network analysis of the Spring/Summer 2007 Fashion Week collections, we explain how producers select models for fashion shows. While fashion producers…

  7. Is the Appropriateness of Emotions Culture-Dependent? The Relevance of Social Meaning

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    Welpinghus Anna

    2018-02-01

    Full Text Available This paper contributes to the question to what extent the socio-cultural context is relevant for the appropriateness of emotions, while appropriateness of an emotion means that the emotion entails a correct, or adequate, evaluation of its object. In a first step, two adequacy conditions for theories of emotions are developed: the first condition ensures that the socio-cultural context is not neglected: theories must allow for the fact that appropriateness often depends on the social meaning of the emotion’s particular object. The second condition rules out implausible forms of cultural determinism. In a second step, an account that meets both conditions is presented: the Acceptance within Social Context account.

  8. Enhanced production and isotope enrichment of recombinant glycoproteins produced in cultured mammalian cells

    Energy Technology Data Exchange (ETDEWEB)

    Skelton, David; Goodyear, Abbey [Florida State University, Department of Chemistry and Biochemistry (United States); Ni, DaQun; Walton, Wendy J.; Rolle, Myron; Hare, Joan T. [Florida State University, Institute of Molecular Biophysics (United States); Logan, Timothy M., E-mail: tlogan@fsu.ed [Florida State University, Department of Chemistry and Biochemistry (United States)

    2010-10-15

    NMR studies of post-translationally modified proteins are complicated by the lack of an efficient method to produce isotope enriched recombinant proteins in cultured mammalian cells. We show that reducing the glucose concentration and substituting glutamate for glutamine in serum-free medium increased cell viability while simultaneously increasing recombinant protein yield and the enrichment of non-essential amino acids compared to culture in unmodified, serum-free medium. Adding dichloroacetate, a pyruvate dehydrogenase kinase inhibitor, further improves cell viability, recombinant protein yield, and isotope enrichment. We demonstrate the method by producing partially enriched recombinant Thy1 glycoprotein from Lec1 Chinese hamster ovary (CHO) cells using U-{sup 13}C-glucose and {sup 15}N-glutamate as labeled precursors. This study suggests that uniformly {sup 15}N,{sup 13}C-labeled recombinant proteins may be produced in cultured mammalian cells starting from a mixture of labeled essential amino acids, glucose, and glutamate.

  9. Enhanced production and isotope enrichment of recombinant glycoproteins produced in cultured mammalian cells

    International Nuclear Information System (INIS)

    Skelton, David; Goodyear, Abbey; Ni, DaQun; Walton, Wendy J.; Rolle, Myron; Hare, Joan T.; Logan, Timothy M.

    2010-01-01

    NMR studies of post-translationally modified proteins are complicated by the lack of an efficient method to produce isotope enriched recombinant proteins in cultured mammalian cells. We show that reducing the glucose concentration and substituting glutamate for glutamine in serum-free medium increased cell viability while simultaneously increasing recombinant protein yield and the enrichment of non-essential amino acids compared to culture in unmodified, serum-free medium. Adding dichloroacetate, a pyruvate dehydrogenase kinase inhibitor, further improves cell viability, recombinant protein yield, and isotope enrichment. We demonstrate the method by producing partially enriched recombinant Thy1 glycoprotein from Lec1 Chinese hamster ovary (CHO) cells using U- 13 C-glucose and 15 N-glutamate as labeled precursors. This study suggests that uniformly 15 N, 13 C-labeled recombinant proteins may be produced in cultured mammalian cells starting from a mixture of labeled essential amino acids, glucose, and glutamate.

  10. Domestically Produced TV-Drama and Cultural Commons

    DEFF Research Database (Denmark)

    From, Unni

    2005-01-01

    The Chapter elaborates empirically some of the aspects and means by which drama can occasion an articulation of cultural commons. Further, it explores explanations why audiences might retain a national perspective as a dominating frame of interpretation even though universal or late-modern interp......-modern interpretation frames would be equally relevant. I argue that institutional policy and politically regulations of media in general, combined with press material and a flow of news articles, chronicles, reviews, interviews and portraits , may establish a dominating paradigm....

  11. Third Culture Kid's Effect on Character of Cady Heron in Mean Girls

    OpenAIRE

    Hapsari, Laily

    2017-01-01

    This thesis focuses on the movie, Mean Girls, specifically one of the character, Cady Heron,as a Third Culture Kid trying to adapt in new society. The purpose of this study is to understand the effect of Third Culture Kid in the movie character in socializing with the new surroundings and explain the way how one of the characters in Mean Girls adapts to the new surroundings.The writer uses library research to collect the data with the novel as the main data, and several relevant books, journa...

  12. THE MEANING IN THE CULTURE: BASIC CONCEPT TO ORGANIZATIONAL LEARNING

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    ÁLVARO ENRÍQUEZ MARTÍNEZ

    2007-01-01

    Full Text Available Based on three key psychological concepts: learning, culture and meaning, the present essay proposes a conceptualbase frame, oriented toward the understanding of development in organizations into current milieu ofcompetitiveness and temporality, of the relationships among people working into them. The organizations whichare typically embedded in a context of values, needs and symbols that made up their cultures, must evolve in orderto face the demands for new developments and change, to which they are forced to. The concept of “meaning of theculture”, is presented as the base over which is build and rooted the organizational learning - in a technical and socialsense. The different types of learning are the ways in which the organizations satisfy the requirements coming fromtheir environments, in front of which must generate knowledge and consequently, new products and services,based on the people that form such organizations. The people that belong and constitutes the organization, incircumstances of temporal cohesion and within temporary working networks, must achieve results and to beadjusted to these new working and organizational facts, developing self-management and autonomy, in order tosignify and get adapted into the cultural tissue.

  13. Integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures.

    Science.gov (United States)

    Patel, Sanjay K S; Kumar, Prasun; Singh, Mamtesh; Lee, Jung-Kul; Kalia, Vipin C

    2015-01-01

    Biological production of hydrogen (H2) and polyhydroxybutyrate (PHB) from pea-shell slurry (PSS) was investigated using defined mixed culture (MMC4, composed of Enterobacter, Proteus, Bacillus spp.). Under batch culture, 19.0LH2/kg of PSS (total solid, TS, 2%w/v) was evolved. Using effluent from the H2 producing stage, Bacillus cereus EGU43 could produce 12.4% (w/w) PHB. Dilutions of PSS hydrolysate containing glucose (0.5%, w/v) resulted in 45-75LH2/kg TS fed and 19.1% (w/w) of PHB content. Under continuous culture, MMC4 immobilized on coconut coir (CC) lead to an H2 yield of 54L/kg TS fed and a PHB content of 64.7% (w/w). An improvement of 2- and 3.7-fold in H2 and PHB yields were achieved in comparison to control. This integrative approach using defined set of bacterial strains can prove effective in producing biomolecules from biowastes. Copyright © 2014. Published by Elsevier Ltd.

  14. HOW DOES THE MEANING THAT CONSUMERS DERIVE FROM ADVERTISEMENTS VARY ACROSS CULTURE?

    OpenAIRE

    Mohanasundaram, Arun Pragash

    2010-01-01

    Due to the convergence of homogenous consumer needs, taste and lifestyle, the globalization of media, incomes and technology were expected. But many researchers debated that adaptation is needed in the place of standardization in many places especially in the field of advertising. The aim of this dissertation is to analyze the influence of culture in advertising and also to show how does the meaning that consumers derive from advertisements, vary across cultures. The influence of culture in a...

  15. Of Models and Meanings: Cultural Resilience in Social-Ecological Systems

    Directory of Open Access Journals (Sweden)

    Todd A. Crane

    2010-12-01

    Full Text Available Modeling has emerged as a key technology in analysis of social-ecological systems. However, the tendency for modeling to focus on the mechanistic materiality of biophysical systems obscures the diversity of performative social behaviors and normative cultural positions of actors within the modeled system. The fact that changes in the biophysical system can be culturally constructed in different ways means that the perception and pursuit of adaptive pathways can be highly variable. Furthermore, the adoption of biophysically resilient livelihoods can occur under conditions that are subjectively experienced as the radical transformation of cultural systems. The objectives of this work are to: (1 highlight the importance of understanding the place of culture within social-ecological systems, (2 explore the tensions between empirical and normative positions in the analysis of social-ecological resilience, and (3 suggest how empirical modeling of social-ecological systems can synergistically interact with normative aspects of livelihoods and lifeways.

  16. Do Between-Culture Differences Really Mean that People Are Different? A Look at Some Measures of Culture Effect Size.

    Science.gov (United States)

    Matsumoto, David; Grissom, Robert J.; Dinnel, Dale L.

    2001-01-01

    Recommends four measures of cultural effect size appropriate for cross-cultural research (standardized difference between two sample means, probabilistic superiority effect size measure, Cohen's U1, and point biserial correlation), demonstrating their efficacy on two data sets from previously published studies and arguing for their use in future…

  17. 'I just said It, I didn't mean anything:' Culture and Pragmatic Inference ...

    African Journals Online (AJOL)

    'I just said It, I didn't mean anything:' Culture and Pragmatic Inference in Interpersonal Communication. Felix Nwabeze Ogoanah, Stella Nkechi Kpolugbo. Abstract. Socio-cultural practices and the economy of expression, which generally characterise human communication, significantly widen the gap between linguistic ...

  18. Three-dimensional neural cultures produce networks that mimic native brain activity.

    Science.gov (United States)

    Bourke, Justin L; Quigley, Anita F; Duchi, Serena; O'Connell, Cathal D; Crook, Jeremy M; Wallace, Gordon G; Cook, Mark J; Kapsa, Robert M I

    2018-02-01

    Development of brain function is critically dependent on neuronal networks organized through three dimensions. Culture of central nervous system neurons has traditionally been limited to two dimensions, restricting growth patterns and network formation to a single plane. Here, with the use of multichannel extracellular microelectrode arrays, we demonstrate that neurons cultured in a true three-dimensional environment recapitulate native neuronal network formation and produce functional outcomes more akin to in vivo neuronal network activity. Copyright © 2017 John Wiley & Sons, Ltd.

  19. Material Culture and Cultural Meanings: Current Studies of Archaeological Ceramics and in Ce­ramic Ethnoarchaeology

    Directory of Open Access Journals (Sweden)

    Charles C. Kolb

    2000-05-01

    Full Text Available Pottery and People: A Dynamic Interaction. James M. Skibo and Gary M. Feinman (editors. Founda­tions of Archaeological Inquiry. Salt Lake City. University of Utah Press. 1999. 260 pp. 91 figures. 30 tables. ISBN 0-87480-576-7. $55.00 (cloth; 0-87489-577-5. $25.00 (paperback. Material Meanings: Critical Approaches to the Interpretation of Material Culture. Elizabeth S. Chilton (editor. Foundations of Archaeological Inquiry. Salt Lake City: University of Utah Press. 1999.179pp. 35 figures. 11 tables. ISBN 0-87480-632-1. $55.00 (cloth; 087480-633-X. $35.00 (paperback. The description and interpretation of material culture may be regarded as the essence of archaeology, a discipline that seeks to recover, describe, document, and interpret past human culture. More recently, understanding that actions occur in a material world that is constituted symbolically, archaeological explanations are often framed in sociocultural meanings, the analysis of agencies, practices and behaviors. I shall subsequently return to this issue. Because of their longevity in the archaeological record, lithic and ceramic artifacts are crucial to the endeavor to interpret human culture. Objects fashioned from clay and subjected to intentional artificial sources of heat made their initial appearance in the archaeological record more than 26.000 years ago. Ceramic objects have been created in a seemingly endless variety of shapes and forms, varying from fertility figurines. to cooking and food storage vessels. lamps, smoking pipes, medicinal pastilles, tokens, beehives, and coffins to modern whitewares and pyroceramics.

  20. Biomass recycle as a means to improve the energy efficiency of CELSS algal culture systems

    Science.gov (United States)

    Radmer, R.; Cox, J.; Lieberman, D.; Behrens, P.; Arnett, K.

    1987-01-01

    Algal cultures can be very rapid and efficient means to generate biomass and regenerate the atmosphere for closed environmental life support systems. However, as in the case of most higher plants, a significant fraction of the biomass produced by most algae cannot be directly converted to a useful food product by standard food technology procedures. This waste biomass will serve as an energy drain on the overall system unless it can be efficiently recycled without a significant loss of its energy content. Experiments are reported in which cultures of the alga Scenedesmus obliquus were grown in the light and at the expense of an added carbon source, which either replaced or supplemented the actinic light. As part of these experiments, hydrolyzed waste biomass from these same algae were tested to determine whether the algae themselves could be made part of the biological recycling process. Results indicate that hydrolyzed algal (and plant) biomass can serve as carbon and energy sources for the growth of these algae, suggesting that the efficiency of the closed system could be significantly improved using this recycling process.

  1. An Empirical Model and Ethnic Differences in Cultural Meanings Via Motives for Suicide.

    Science.gov (United States)

    Chu, Joyce; Khoury, Oula; Ma, Johnson; Bahn, Francesca; Bongar, Bruce; Goldblum, Peter

    2017-10-01

    The importance of cultural meanings via motives for suicide - what is considered acceptable to motivate suicide - has been advocated as a key step in understanding and preventing development of suicidal behaviors. There have been limited systematic empirical attempts to establish different cultural motives ascribed to suicide across ethnic groups. We used a mixed methods approach and grounded theory methodology to guide the analysis of qualitative data querying for meanings via motives for suicide among 232 Caucasians, Asian Americans, and Latino/a Americans with a history of suicide attempts, ideation, intent, or plan. We used subsequent logistic regression analyses to examine ethnic differences in suicide motive themes. This inductive approach of generating theory from data yielded an empirical model of 6 cultural meanings via motives for suicide themes: intrapersonal perceptions, intrapersonal emotions, intrapersonal behavior, interpersonal, mental health/medical, and external environment. Logistic regressions showed ethnic differences in intrapersonal perceptions (low endorsement by Latino/a Americans) and external environment (high endorsement by Latino/a Americans) categories. Results advance suicide research and practice by establishing 6 empirically based cultural motives for suicide themes that may represent a key intermediary step in the pathway toward suicidal behaviors. Clinicians can use these suicide meanings via motives to guide their assessment and determination of suicide risk. Emphasis on environmental stressors rather than negative perceptions like hopelessness should be considered with Latino/a clients. © 2017 Wiley Periodicals, Inc.

  2. Cellular modifications produced by D2O in yeast culture

    International Nuclear Information System (INIS)

    Mihancea, I.; Mircea, R.Al.

    1996-01-01

    The cellular cycle of the Saccharomyces Cerevisiae, chosen as experimental object, is unmodified by the presence in the culture medium of D 2 O at different concentrations. An increased concentration of D 2 O in the culture medium leads to a decrease of the number of budded cells, to metabolic alterations, to DNA structure modification as well as to enzymatic changes produced by blocking. Other anomalies appear, as a function of the cell defence capacity and of the influence of the factors from the nutrient substrate or exterior medium. Due to D 2 O, the medium's pH changes and modifications at the enzyme level and of the cell microstructure and morphology occur. The enzymatic reactions take place in D 2 O slower than in H 2 O. Three-dimensional modifications appear in the organic components of the live cell which, in turn, produce profound modifications in the cell growth and division. Due to the kinetic and isotopic effects, modifications of the biochemical reactions affecting the cell integrity happen

  3. Cultural meanings of musculoskeletal diseases in Chile's Mapuche Population.

    Science.gov (United States)

    Alarcon, Ana M; Vidal, Aldo; Castro, Marcela

    2013-10-01

    Eight out of 10 Mapuche indigenous women have a musculoskeletal disorder (MSD) and do not seek early medical aid. To study both the cultural meanings and implications of MSD from the Mapuche worldview. Ethnographic study carried out from 2008 to 2011 on indigenous reserves in southern Chile. Sixty-four Mapuches participated in comprehensive interviews, which were transcribed and analyzed by the research team. Five cultural domains: (a) foro kutran/bone disease is the general denomination of MSD; (b) Re-Rume Kutran/progressive and incurable course, refers to the course of the disease; (c) Kalül fücha mawiza/body is an old tree, describes internal manifestations such as worn bones, dry body, weak blood, and spiritual weakness; (d) witrür tripai foro/deformation is the external manifestation of MSD; and (e) Reñma ka lof kutran/family and community suffering refers to the impact of MSD. The explanation of MSD is consistent with the integrated body-nature-spirit worldview of the Mapuche. To provide cultural nursing health care so that patients receive prompt diagnosis and care.

  4. Crossing the cultural divide: issues in translation, mistrust, and cocreation of meaning in cross-cultural therapeutic assessment.

    Science.gov (United States)

    Rosenberg, Audrey; Almeida, Angelica; Macdonald, Heather

    2012-01-01

    This article examines cross-cultural therapeutic assessment in a community mental health clinic. The first case describes the work between a Caucasian assessor and a Mexican American family. The authors explore the metaphorical and literal translation of the findings from English to Spanish and the parallel process of translation of the self, experienced by both assessor and client. The second case describes the work between a Caucasian assessor and an African American adolescent. We describe the inherent challenge between the Eurocentric "task" orientation of the evaluation and the Afrocentric "relationship" orientation. We suggest that bridging the gap between cultures and overcoming cultural mistrust lay in the building of the assessor-client relationship. Fischer's concepts of rapport and intimacy are emphasized and expanded on as we emphasize the importance of cocreated meaning in cross-cultural assessment work.

  5. Clusters of cultures: diversity in meaning of family value and gender role items across Europe.

    Science.gov (United States)

    van Vlimmeren, Eva; Moors, Guy B D; Gelissen, John P T M

    2017-01-01

    Survey data are often used to map cultural diversity by aggregating scores of attitude and value items across countries. However, this procedure only makes sense if the same concept is measured in all countries. In this study we argue that when (co)variances among sets of items are similar across countries, these countries share a common way of assigning meaning to the items. Clusters of cultures can then be observed by doing a cluster analysis on the (co)variance matrices of sets of related items. This study focuses on family values and gender role attitudes. We find four clusters of cultures that assign a distinct meaning to these items, especially in the case of gender roles. Some of these differences reflect response style behavior in the form of acquiescence. Adjusting for this style effect impacts on country comparisons hence demonstrating the usefulness of investigating the patterns of meaning given to sets of items prior to aggregating scores into cultural characteristics.

  6. Experimental and mathematical model of the interactions in the mixed culture of links in the "producer-consumer" cycle

    Science.gov (United States)

    Pisman, T. I.; Galayda, Ya. V.

    The paper presents experimental and mathematical model of interactions between invertebrates the ciliates Paramecium caudatum and the rotifers Brachionus plicatilis and algae Chlorella vulgaris and Scenedesmus quadricauda in the producer -- consumer aquatic biotic cycle with spatially separated components The model describes the dynamics of the mixed culture of ciliates and rotifers in the consumer component feeding on the mixed algal culture of the producer component It has been found that metabolites of the algae Scenedesmus produce an adverse effect on the reproduction of the ciliates P caudatum Taking into account this effect the results of investigation of the mathematical model were in qualitative agreement with the experimental results In the producer -- consumer biotic cycle it was shown that coexistence is impossible in the mixed algal culture of the producer component and in the mixed culture of invertebrates of the consumer component The ciliates P caudatum are driven out by the rotifers Brachionus plicatilis

  7. Culture care meanings, beliefs, and practices in Rural Dominican Republic.

    Science.gov (United States)

    Schumacher, Gretchen

    2010-04-01

    This ethnonursing study explored the meanings, beliefs, and practices of care for rural Dominicans in the Dominican Republic. Leininger's culture care diversity and universality theory, ethnonursing, and four-phase analysis method guided the study. Interviews were conducted with 19 general and 10 key informants. Analysis of interviews revealed three main themes: (a) family presence is essential for meaningful care experiences and care practices, (b) respect and attention are central to the meaning of care and care practices, and (c) rural Dominicans value and use both generic (folk) and professional care practices. Implications and recommendations for nursing practice, education, and research are described.

  8. Bishnuprasad Rabha as Cultural Icon of Assam: The Process of Meaning Making

    Directory of Open Access Journals (Sweden)

    Parismita Hazarika

    2017-03-01

    Full Text Available Abstract The term ‘cultural icon’ is generally used to refer to individuals or images, objects, visual sign, monuments, space etc. In semiotics the term ‘icon’ is used to refer to a sign that bears close resemblance to the object that it stands for. Icons are particularly influential signifiers because they are immediately identifiable and carry complex cultural codes in a compact image. In this paper the understanding of ‘cultural icon’ is not limited to semiotics. Following Keyan Tomaselli and David Scott in Cultural Icons (2009, we believe that cultural icons are purposive constructions. An attempt has been made in this paper to analyze the association of ‘desirable’ meanings to a cultural icon (while dropping ‘undesirable’ ones; thus, it is imperative that we look at the changing socio-political contexts behind such purposive constructions. With this in mind, we look at the iconic figure of Bishnuprasad Rabha who has been one of the most revered figures in the cultural history of Assam and has been appropriated as a cultural icon in different discourses of the national life of Assam that has emerged in recent times.

  9. Children's activities and their meanings for parents: a mixed-methods study in six Western cultures.

    Science.gov (United States)

    Harkness, Sara; Zylicz, Piotr Olaf; Super, Charles M; Welles-Nyström, Barbara; Bermúdez, Moisés Ríos; Bonichini, Sabrina; Moscardino, Ughetta; Mavridis, Caroline Johnston

    2011-12-01

    Theoretical perspectives and research in sociology, anthropology, sociolinguistics, and cultural psychology converge in recognizing the significance of children's time spent in various activities, especially in the family context. Knowing how children's time is deployed, however, only gives us a partial answer to how children acquire competence; the other part must take into account the culturally constructed meanings of activities, from the perspective of those who organize and direct children's daily lives. In this article, we report on a study of children's routine daily activities and on the meanings that parents attribute to them in six Western middle-class cultural communities located in Italy, The Netherlands, Poland, Spain, Sweden, and the United States (N = 183). Using week-long time diaries kept by parents, we first demonstrate similarities as well as significant differences in children's daily routines across the cultural samples. We then present brief vignettes--"a day in the life" --of children from each sample. Parent interviews were coded for themes in the meanings attributed to various activities. Excerpts from parent interviews, focusing on four major activities (meals, family time, play, school- or developmentally related activities), are presented to illustrate how cultural meanings and themes are woven into parents' organization and understanding of their children's daily lives. The results of this mixed-method approach provide a more reliable and nuanced picture of children's and families' daily lives than could be derived from either method alone.

  10. Resveratrol Produces Neurotrophic Effects on Cultured Dopaminergic Neurons through Prompting Astroglial BDNF and GDNF Release

    Directory of Open Access Journals (Sweden)

    Feng Zhang

    2012-01-01

    Full Text Available Increasing evidence indicated astroglia-derived neurotrophic factors generation might hold a promising therapy for Parkinson’s disease (PD. Resveratrol, naturally present in red wine and grapes with potential benefit for health, is well known to possess a number of pharmacological activities. Besides the antineuroinflammatory properties, we hypothesized the neuroprotective potency of resveratrol is partially due to its additional neurotrophic effects. Here, primary rat midbrain neuron-glia cultures were applied to investigate the neurotrophic effects mediated by resveratrol on dopamine (DA neurons and further explore the role of neurotrophic factors in its actions. Results showed resveratrol produced neurotrophic effects on cultured DA neurons. Additionally, astroglia-derived neurotrophic factors release was responsible for resveratrol-mediated neurotrophic properties as evidenced by the following observations: (1 resveratrol failed to exert neurotrophic effects on DA neurons in the cultures without astroglia; (2 the astroglia-conditioned medium prepared from astroglia-enriched cultures treated with resveratrol produced neurotrophic effects in neuron-enriched cultures; (3 resveratrol increased neurotrophic factors release in the concentration- and time-dependent manners; (4 resveratrol-mediated neurotrophic effects were suppressed by blocking the action of the neurotrophic factors. Together, resveratrol could produce neurotrophic effects on DA neurons through prompting neurotrophic factors release, and these effects might open new alternative avenues for neurotrophic factor-based therapy targeting PD.

  11. Overcoming the Discontinuity of Patriotic Education and Moral Development by Means of Regional Culture

    Science.gov (United States)

    Tikhonova, Anna U.

    2016-01-01

    The paper analyzes studies on continuing education, explains the concept of "continuity of patriotic education and moral development by means of regional culture", determines the uniqueness of regional culture as the core of patriotic education and moral development, and characterizes the model of continuity of patriotic education and…

  12. The Cultural Voice of Immigrant Latina Women and the Meaning of Femininity

    Directory of Open Access Journals (Sweden)

    Ellen Greer

    2013-04-01

    Full Text Available This phenomenological study investigates the cultural meaning of femininity to immigrant Latina women and its significance in the consideration of decisions related to maintaining breast health. Theories of culture and health promotion support the concern for women’s need for access to health care in relation to breast health and the cultural barriers that interface between the connections of femininity, body image, and mental health. For Hispanic women, breast cancer is the most commonly diagnosed cancer generating distress for the individual, partner, and family. The importance of best practices in health promotion and risk reduction strategies in the early screening of women is emphasized in the literature. However, the voice of immigrant Latina women in describing their perspective of femininity and breast health and how it may affect choice and decision making related to breast self-care practices has not been studied. Five themes emerged from the data analysis conducted with Giorgi’s phenomenological method leading to an unfolded description of femininity: power of feminine identity through motherhood, hardiness is sustainability to overcoming adversity, connection to self and others, satisfaction meeting cultural gender-role expectations, and contemplative prevention to maintain breast integrity. The findings of this study will contribute to the increasing body of evidence-based practice related to understanding the impact of culture related to breast health. While the concept of femininity can be challenged not only by a diagnosis of breast cancer but also by other diseases and life conditions, understanding the cultural meaning of femininity to Latina women is pivotal to health care professionals as they partner with Latina women and community support groups to develop empowerment strategies and programs that promote choice and decision making involving breast health.

  13. Religion as memory: How has the continuity of tradition produced collective meanings? – Part one

    Directory of Open Access Journals (Sweden)

    Jakub Urbaniak

    2015-06-01

    Full Text Available Danièle Hervieu-Léger gives an account of religion as a chain of memory, that is, a form of collective memory and imagination based on the sanctity of tradition. According to her theory, in the postmodern world the continuity of religious memory has been broken and all that remains are isolated fragments guarded by religious groups. This twofold study aims at showing, firstly, in what sense religion can be conceived of as memory which produces collective meanings (Part One and, secondly, what may happen when individualised and absolutised memories alienate themselves from a continuity of tradition, thus beginning to function as a sort of private religion (Part Two. Being the first part of the study in question, this article is dedicated to a historical-theological analysis of religious memory as a source of collective meanings, as seen from a Christian perspective. Firstly, it situates Hervieu-Léger’s definition of religion against the background of the most topical religious contexts in which the notion of memory appears today. Secondly, the dialectics of individual and collective memory is discussed, notably through the lens of Ricoeur’s original proposal. This is followed by an overview of the traditional functions of memory in Christianity. Lastly, the interpretation of the way in which Christian tradition, in its premodern continuity, served as a source of collective cultural meanings, is recapitulated. What underlies this analysis is the conviction that to comprehend, and even more so to challenge mechanisms based on which the dominant purveyors of meaning (such as economic and information market function in our day, one should have a clear understanding of what they attempt to substitute for. In brief, before exploring how memories become religion, one ought to be able to conceive of religion as memory.

  14. A rapid radioassay for human IgG produced in lymphocyte in vitro culture systems

    International Nuclear Information System (INIS)

    Weightman, D.R.; Shale, D.J.; Tomlinson, W.R.

    1981-01-01

    Protein A bearing Staphylococcus aureus was used to develop a solid-phase radioassay for IgG immunoglobulins. The assay was specifically optimised for use in vitro human lymphocyte culture work. Compared with a solid-phase radioimmunoassay for IgG produced in lymphocyte culture, this assay had a similar performance profile and the advantages of rapidity and technical ease. (Auth.)

  15. Training Culture: A New Conceptualization to Capture Values and Meanings of Training in Organizations

    Science.gov (United States)

    Polo, Federica; Cervai, Sara; Kantola, Jussi

    2018-01-01

    Purpose: The purpose of this study is to introduce and validate the concept of training culture defined as a subset of the main organizational culture that allows examining meanings and values attributed to the training within an organization by management and employees. Design/methodology/approach: This study, following the deductive scale…

  16. Culture care meanings and experiences of postpartum depression among Jordanian Australian women: a transcultural study.

    Science.gov (United States)

    Nahas, V; Amasheh, N

    1999-01-01

    This study discovers, describes, and explains the personal experiences, perceptions, and care meanings of Jordanian women who have suffered postpartum depression. Most postpartum cases often are misdiagnosed as exclusively psychological and untreated by health care professionals without consideration to the cultural meanings of this problem. Understanding the experiences of these women is important, as their expressions often are contextually and culturally influenced. Using Leininger's Theory of Culture Care Diversity and Universality, a purposive sample of 22 Jordanian women diagnosed with postpartum depression, living in Sydney, were interviewed. The ethnonursing research method and data analysis procedures were used. Results revealed that Jordanian mothers experienced severe loss of control over emotions of loneliness, hopelessness, and feelings of being a bad mother. Three major themes focusing on the care meanings and experiences of Jordanian women are discussed: (a) Care means strong family support and kinship during the postpartum period, (b) care is carrying out and fulfilling traditional gender roles as mother and wife, and (c) care is preservation of Jordanian childbearing customs as expressed in the celebration of the birth of the baby.

  17. [Diversity and enzyme-producing activity of culturable halophilic bacteria in Daishan Saltern of East China].

    Science.gov (United States)

    Yang, Dan-Dan; Li, Qian; Huang, Jing-Jing; Chen, Min

    2012-11-01

    Soil and saline water samples were collected from the Daishan Saltern of East China, and the halophilic bacteria were isolated and cultured by using selective media, aimed to investigate the diversity and enzyme-producing activity of culturable halophilic bacteria in saltern environment. A total of 181 strains were isolated by culture-dependent method. Specific primers were used to amplify the 16S rRNA gene of bacteria and archaea. The operation taxonomy units (OTUs) were determined by ARDRA method, and the representative strain of each OTU was sequenced. The phylogenetic position of all the isolated strains was determined by 16S rRNA sequencing. The results showed that the isolated 181 strains displayed 21 operational taxonomic units (OTUs), of which, 12 OTUs belonged to halophilic bacteria, and the others belonged to halophilic archaea. Phylogenetic analysis indicated that there were 7 genera presented among the halophilic bacteria group, and 4 genera presented among the halophilic archaea group. The dominant halophilic strains were of Halomonas and Haloarcula, with 46.8% in halophilic bacteria and 49.1% in halophilic archaea group, respectively. Enzyme-producing analysis indicated that most strains displayed enzyme-producing activity, including the activities of producing amylase, proteinase and lipase, and the dominant strains capable of enzyme-producing were of Haloarcula. Our results showed that in the environment of Daishan Saltern, there existed a higher diversity of halophilic bacteria, being a source sink for screening enzyme-producing bacterial strains.

  18. Experiments with a First Prototype of a Spatial Model of Cultural Meaning through Natural-Language Human-Robot Interaction

    Directory of Open Access Journals (Sweden)

    Oliver Schürer

    2018-01-01

    Full Text Available When using assistive systems, the consideration of individual and cultural meaning is crucial for the utility and acceptance of technology. Orientation, communication and interaction are rooted in perception and therefore always happen in material space. We understand that a major problem lies in the difference between human and technical perception of space. Cultural policies are based on meanings including their spatial situation and their rich relationships. Therefore, we have developed an approach where the different perception systems share a hybrid spatial model that is generated by artificial intelligence—a joint effort by humans and assistive systems. The aim of our project is to create a spatial model of cultural meaning based on interaction between humans and robots. We define the role of humanoid robots as becoming our companions. This calls for technical systems to include still inconceivable human and cultural agendas for the perception of space. In two experiments, we tested a first prototype of the communication module that allows a humanoid to learn cultural meanings through a machine learning system. Interaction is achieved by non-verbal and natural-language communication between humanoids and test persons. This helps us to better understand how a spatial model of cultural meaning can be developed.

  19. Ammonia produced by bacterial colonies promotes growth of ampicillin-sensitive Serratia sp. by means of antibiotic inactivation.

    Science.gov (United States)

    Cepl, Jaroslav; Blahůšková, Anna; Cvrčková, Fatima; Markoš, Anton

    2014-05-01

    Volatiles produced by bacterial cultures are known to induce regulatory and metabolic alterations in nearby con-specific or heterospecific bacteria, resulting in phenotypic changes including acquisition of antibiotic resistance. We observed unhindered growth of ampicillin-sensitive Serratia rubidaea and S. marcescens on ampicillin-containing media, when exposed to volatiles produced by dense bacterial growth. However, this phenomenon appeared to result from pH increase in the medium caused by bacterial volatiles rather than alterations in the properties of the bacterial cultures, as alkalization of ampicillin-containing culture media to pH 8.5 by ammonia or Tris exhibited the same effects, while pretreatment of bacterial cultures under the same conditions prior to antibiotic exposure did not increase ampicillin resistance. Ampicillin was readily inactivated at pH 8.5, suggesting that observed bacterial growth results from metabolic alteration of the medium, rather than an active change in the target bacterial population (i.e. induction of resistance or tolerance). However, even such seemingly simple mechanism may provide a biologically meaningful basis for protection against antibiotics in microbial communities growing on semi-solid media. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  20. Quantitative and qualitative research across cultures and languages: cultural metrics and their application.

    Science.gov (United States)

    Wagner, Wolfgang; Hansen, Karolina; Kronberger, Nicole

    2014-12-01

    Growing globalisation of the world draws attention to cultural differences between people from different countries or from different cultures within the countries. Notwithstanding the diversity of people's worldviews, current cross-cultural research still faces the challenge of how to avoid ethnocentrism; comparing Western-driven phenomena with like variables across countries without checking their conceptual equivalence clearly is highly problematic. In the present article we argue that simple comparison of measurements (in the quantitative domain) or of semantic interpretations (in the qualitative domain) across cultures easily leads to inadequate results. Questionnaire items or text produced in interviews or via open-ended questions have culturally laden meanings and cannot be mapped onto the same semantic metric. We call the culture-specific space and relationship between variables or meanings a 'cultural metric', that is a set of notions that are inter-related and that mutually specify each other's meaning. We illustrate the problems and their possible solutions with examples from quantitative and qualitative research. The suggested methods allow to respect the semantic space of notions in cultures and language groups and the resulting similarities or differences between cultures can be better understood and interpreted.

  1. The role of acid incubation in rapid immobilization of hydrogen-producing culture in anaerobic upflow column reactors

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Zhen-Peng; Tay, Joo-Hwa [School of Civil and Environmental Engineering, Nanyang Technological University (Singapore); Institute of Environmental Science and Engineering, Nanyang Technological University (Singapore); Show, Kuan-Yeow [Faculty of Science, Engineering and Technology, University Tunku Abdul Rahman, 31900 Kampar, Perak (Malaysia); Liang, David Tee [Institute of Environmental Science and Engineering, Nanyang Technological University (Singapore); Lee, Duu-Jong [Department of Chemical Engineering, National Taiwan University, Taipei 10617 (China); Su, Ay [Department of Mechanical Engineering, Fuel Cell Center, Yuan-Ze University, Taoyuan 320 (China)

    2008-10-15

    An approach of acidification was examined on formation of hydrogen-producing granules and biofilms in upflow column-shaped reactors. The reactors were fed with synthetic glucose wastewater and operated at 37 C and pH 5.5. The acclimated anaerobic culture was inoculated in four reactors designated R1, R2, R3 and R4, with R3 and R4 filled with granular activated carbon as support medium. To unveil the roles of acidification, microbial culture in R2 and R3 was subject to an acid incubation for 24 h by shifting the culture pH from 5.5 to 2.0. The experimental results suggested that the acidification substantially accelerated microbial granulation, but not biofilm formation. Microbial activities were inhibited by the acid incubation for about 78 h, resulting in the retarded formation of biofilms of the acidified culture. Reducing culture pH resulted in improvement in cell surface physicochemical properties favoring microbial adhesion and immobilization. Zeta potential increased from -25.3 mV to 11.9 mV, hydrophobicity in terms of contact angle improved from 31 to 38 and production of extracellular polymers increased from 66 mg/g-VSS to 136 mg/g-VSS. As a result of the formation of granules and biofilms, high hydrogen production rates of 6.98 and 7.49 L/L h were achieved in granule-based and biofilm-based reactors, respectively. It is concluded that acid incubation is an efficient means to initiate the rapid formation of granules by regulating the surface characteristics of microbial culture. The use of support media as starting nuclei may result in rapid formation of biofilms without the acidification. (author)

  2. The role of acid incubation in rapid immobilization of hydrogen-producing culture in anaerobic upflow column reactors

    International Nuclear Information System (INIS)

    Zhang, Zhen-Peng; Tay, Joo-Hwa; Show, Kuan-Yeow; Liang, David Tee; Lee, Duu-Jong; Su, Ay

    2008-01-01

    An approach of acidification was examined on formation of hydrogen-producing granules and biofilms in upflow column-shaped reactors. The reactors were fed with synthetic glucose wastewater and operated at 37 C and pH 5.5. The acclimated anaerobic culture was inoculated in four reactors designated R1, R2, R3 and R4, with R3 and R4 filled with granular activated carbon as support medium. To unveil the roles of acidification, microbial culture in R2 and R3 was subject to an acid incubation for 24 h by shifting the culture pH from 5.5 to 2.0. The experimental results suggested that the acidification substantially accelerated microbial granulation, but not biofilm formation. Microbial activities were inhibited by the acid incubation for about 78 h, resulting in the retarded formation of biofilms of the acidified culture. Reducing culture pH resulted in improvement in cell surface physicochemical properties favoring microbial adhesion and immobilization. Zeta potential increased from -25.3 mV to 11.9 mV, hydrophobicity in terms of contact angle improved from 31 to 38 and production of extracellular polymers increased from 66 mg/g-VSS to 136 mg/g-VSS. As a result of the formation of granules and biofilms, high hydrogen production rates of 6.98 and 7.49 L/L h were achieved in granule-based and biofilm-based reactors, respectively. It is concluded that acid incubation is an efficient means to initiate the rapid formation of granules by regulating the surface characteristics of microbial culture. The use of support media as starting nuclei may result in rapid formation of biofilms without the acidification. (author)

  3. Spreadable media: Creating value and meaning in a networked culture

    Directory of Open Access Journals (Sweden)

    Moderated by Louisa Stein

    2014-09-01

    Full Text Available Online Roundtable on Spreadable Media, by Henry Jenkins, Sam Ford, and Joshua Green, with participants Paul Booth, Kristina Busse, Melissa Click, Sam Ford, Henry Jenkins, Xiaochang Li, and Sharon Ross. Section 1 first published as the article "Spreadable Media: Creating Value and Meaning in a Networked Culture", by Louisa Stein, from Cinema Journal Volume 53 Issue 3, pp152-177. Copyright 2014 by The University of Texas Press. All rights reserved.

  4. The new frontiers for CCT: Considering consumer-producer interaction in consumer culture

    NARCIS (Netherlands)

    MSc Lotte Salome

    2010-01-01

    Previous investigations of consumer subcultures in the CCT tradition focused primarily on consumer behaviours, feelings, experiences and meanings of consumption. This paper advocates that in order to deeply understand and interpret a particular subculture, researchers in consumer culture should

  5. SOCIO-CULTURAL COMPETENCE FORMATION BY MEANS OF TRANSLATION IN THE CONDITIONS OF INCLUSIVE EDUCATION

    Directory of Open Access Journals (Sweden)

    Kateryna Shapochka

    2016-12-01

    Full Text Available The article is devoted to the problem of socio-cultural competence formation by means of translation / interpretation and the necessity of foreign language communicative competence formation in the process of inclusive education. The question of training of young generation for life in a multi-ethnic and multicultural society, forming skills of communication and cooperation with people of different nationalities, the foreign language learning, the formation of the communicative and socio-cultural competence is one of the main tasks of modern school to meet educational needs persons with disabilities. Today’s realities require that students with special educational needs should study a foreign language and use it in the process of learning. In turn, the use of translation in the process of learning a foreign language helps students to get new skills, to form general and specific competences, including socio-cultural competence, which promotes socialization of children with special needs, and integrating them into a comprehensive system of Ukraine. The article raises the problem of modernization of the educational system. It was established that the formation of socio-cultural competence by means of written translation is done by means of a system of exercises. Based on this system, subsystems, groups and types of exercises their systems can be developed in accordance with human activity, objectives and learning environment. It shows that the development of an inclusive approach to learning demands new solutions towards learning a foreign language at different levels of education.

  6. Using Pure Cultures to Define the Site Preference of Nitrous Oxide Produced by Microbial Nitrification and Denitrification

    Science.gov (United States)

    Sutka, R. L.; Breznak, J. A.; Ostrom, N. E.; Ostrom, P. H.; Gandhi, H.

    2004-12-01

    Defining the site preference of nitrous oxide (N2O) produced in pure culture studies is crucial to interpreting field data. We have previously demonstrated that the intramolecular distribution of nitrogen isotopes (isotopomers) can be used to differentiate N2O produced by nitrifier denitrification and nitrification in cultures of Nitrosomonas europaea. Here, we have expanded on our initial results and evaluated the isotopomeric composition of N2O produced during nitrification and nitrifier denitrification with cultures of Nitrosospira multiformis. In addition, we have analyzed N2O produced during methanotrophic nitrification, denitrification, and fungal denitrification. To evaluate N2O production during nitrification and nitrifier denitrification, we compared the site preference of N2O formed as a result of nitrite reduction and hydroxylamine oxidation with Nitrosomonas europaea and Nitrosospira multiformis. The average site preference of N2O produced by hydroxylamine oxidation was similar for Nitrosomonas europaea (33.0 ± 3.5 ‰ ) and Nitrosospira multiformis (33.1 ± 4.2 ‰ ). Nitrous oxide produced by nitrifier-denitrification by Nitrosomonas europaea and Nitrosospira multiformis had a similar site preference of - 1.4 ± 4.4 ‰ and - 1.1 ± 2.6 ‰ respectively. The results indicate that it is possible to differentiate between N2O produced by nitrite reduction and hydroxylamine oxidation by ammonia oxidizing bacteria. Methanotrophic nitrification was evaluated by analyzing the N2O produced during hydroxylamine oxidation in concentrated cell suspensions of two methane oxidizing bacteria. The site preference of N2O produced by the two methane oxidizers, Methylococcus capsulatus Bath and Methylosinus trichosporium was 31.8 ± 4.7 ‰ and 33.0 ± 4.5 ‰ respectively. The results indicate that a site preference of 33 ‰ is applicable for nitrification regardless of whether a methane oxidizer or ammonia oxidizer is involved in the reaction. To determine the site

  7. Mutual expectation compacts: a means to link practice culture and vision.

    Science.gov (United States)

    Drury, Ivo; Slomski, Carol

    2007-01-01

    We describe the development of a mutual expectation compact in an academic department of surgery as a means of reinforcing the department's vision document and promoting cultural change. The compact makes explicit those implicit agreements that exist between a physician and his or her practice. It strengthens the relationship by avoiding the misunderstandings that can arise when agreements are implicit.

  8. Translation and Culture:Translation as a Cross-cultural Mediation

    Institute of Scientific and Technical Information of China (English)

    叶谋锦

    2013-01-01

    Translation is a complex activity which involves language competence as well as proficiency in multiculturalism. From the perspective of multiculturalism, translation resembles recreation of source text by grasping essential meanings to produce a sub-tle target text which can be clearly perceived by target readers. Ignoring cultural issues can present serious mistranslations in the field of advertising translation. This paper aims to explore the significance of connotation confined by the framework of culture and point out that verbal translation is a dangerous inclination by illustrating three business examples. This paper argues that cross-cultural mediation plays an important role in translation.

  9. The Meaning of English Words across Cultures, with a Focus on Cameroon and Hong Kong

    Science.gov (United States)

    Bobda, Augustin Simo

    2009-01-01

    A word, even when considered monosemic, generally has a cluster of meanings, depending on the mental representation of the referent by the speaker/writer or listener/reader. The variation is even more noticeable across cultures. This paper investigates the different ways in which cultural knowledge helps in the interpretation of English lexical…

  10. Isolation of rhamnolipids-producing cultures from faeces: Influence of interspecies communication on the yield of rhamnolipid congeners.

    Science.gov (United States)

    Woźniak-Karczewska, Marta; Myszka, Kamila; Sznajdrowska, Agata; Szulc, Alicja; Zgoła-Grześkowiak, Agnieszka; Ławniczak, Łukasz; Corvini, Philippe F-X; Chrzanowski, Łukasz

    2017-05-25

    The aim of this study was to evaluate the ability of bacterial cultures isolated from cattle, poultry or pig faeces and manure to produce rhamnolipids, as well as to investigate the influence of interspecies communication on possible quantitative differences in the production of rhamnolipid congeners. Initial screening methods (oil spreading, drop collapse, haemolytic activity and emulsification activity) showed that approximately 36% of the 51 isolated cultures exhibited the ability to produce biosurfactants. Subsequent studies using a selected culturable mixed culture (which included Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli) revealed that only P. aeruginosa was able to produce this biosurfactant. HPLC-MS analysis showed that the surface active compounds were rhamnolipids. Further comparative studies confirmed that the total yield of rhamnolipids was notably higher in the bioreactor inoculated with the selected mixed culture (940.58±1.10mg/L) compared to the bioreactor inoculated with the axenic strain of P. aeruginosa (108.47±0.41mg/L). Twelve rhamnolipid congeners were identified during cultivation of the selected mixed culture, whereas six congeners were detected during cultivation of the sole axenic strain of P. aeruginosa. Furthermore, increased production of rhamnolipids was observed when the concentration of autoinducer molecules (AI-2) responsible for interspecies signaling increased, suggesting the influence of quorum-sensing communication on biosynthesis efficiency. This observation may be of importance for large-scale production of this biosurfactant, as it opens new possible solutions based on the use of mixed cultures or external addition of stimulating autoinducers. Copyright © 2016 Elsevier B.V. All rights reserved.

  11. Narratives of continued formation: meanings produced by Physical Education Teachers

    Directory of Open Access Journals (Sweden)

    André da Silva Mello

    2015-06-01

    Full Text Available It discusses the meanings given by 14 physical education teachers about their experiences with the continued formation process. It is a (auto biographical narrative research, which used focal groups and interviews as tools for compiling the data. The colaborators are six male teachers and eight female teachers of municipal networks from Serra, Vitoria and Viana municipalities in the metropolitan region of Vitória, ES. The analyzes indicate that the assigned meanings are related to the collective need to reorganize institutional provided formations in dialogue with the political interests of teachers and the specificity of Physical Education as a curriculum component. In general, teachers consider the formation focused on practice and in the teaching profession as those that best reflect their expectations. The practice appears as a know-how to be appropriate from continued formetion to be experienced in school; a knowledge that, when apprehended, is reframed to produce other experiences.

  12. Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali.

    Science.gov (United States)

    Sangare, Samba Adama; Maiga, Almoustapha Issiaka; Guindo, Ibrehima; Maiga, Aminata; Camara, Namory; Dicko, Oumar Agaly; Diallo, Souleymane; Bougoudogo, Flabou; Armand-Lefevre, Laurence; Andremont, Antoine; Maiga, Ibrahim Izetiegouma

    2016-10-31

    The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients' blood cultures in two university teaching hospitals of Bamako, Mali. During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated. The microbial identifications were initially performed with an API 20E gallery and VITEK2 locally in Mali, and then confirmation in France was performed with a mass spectrometry MALDI-TOF in the bacteriology laboratory of the university teaching hospital of Bichat. Antibiotic susceptibility profiles were determined by the diffusion method as recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The isolated species were K. pneumoniae (14/40; 35.0%), E. coli (11/40; 27.5%), and E. cloacae (9/40; 22.5%). Of the strains isolated, 21/34 (61.8%) had an ESBL phenotype, including 10/14 (71.4%) K. pneumoniae, 8/11 (72.7%) E. coli, and 3/9 (33.3%) E. cloacae. Resistances associated with ESBL strains of K. pneumoniae, E. coli, and E. cloacae were as follows: gentamicin (10/10, 100%; 6/8, 75%; 2/3, 67%, respectively), amikacin (2/10, 20%; 0/8, 0%; 0/3, 0%, respectively), ofloxacin (8/10, 80%; 7/8, 87%; 3/3, 100%, respectively), and cotrimoxazole (10/10, 100%; 6/8, 75%; 3/3, 100%, respectively). Almost two-thirds (61.8%) of Enterobacteriaceae isolated from our blood cultures were ESBL producers. Only susceptibilities to carbapenems and to amikacin were fully conserved within the strains.

  13. When does meaning making predict subjective well-being? Examining young and older adults in two cultures.

    Science.gov (United States)

    Alea, Nicole; Bluck, Susan

    2013-01-01

    Two studies in different cultures (Study 1: USA, N=174, Study 2: Trinidad, N=167) examined whether meaning making, (i.e., both searching for meaning, and directing behaviour) is positively related to subjective well-being (SWB) by age (younger, older adults). In both studies, participants self-reported engagement in meaning making, and SWB (e.g., affect, future time perspective, psychological well-being). In Study 1, young Americans (compared to older) more frequently used their past to direct behaviour but doing so was unrelated to SWB. In older Americans, both types of meaning making were positively associated with SWB. In Study 2, Trinidadian younger adults were again more likely than older adults to engage in meaning making. Unlike in the American sample, however, directing behaviour was positively related to SWB for both young and older adults. The studies demonstrate that whether meaning making shows benefits for SWB may depend on type of meaning, age and culture. Note that although meaning making was sometimes unrelated to SWB, no detrimental relations to meaning making were found. The discussion focuses on the role of moderators in understanding when meaning making should lead to benefits versus costs to SWB.

  14. Increasing the production of desulfurizing biocatalysts by means of fed - batch culture

    International Nuclear Information System (INIS)

    Berdugo, C I; Mena, J A; Acero, J R; Mogollon, L

    2001-01-01

    Over the past years, environmental regulations have driven a lot of effort for the development of new technologies for the upgrading of fossil fuels. Biotechnology offers an alternative way to process fossil fuels by means of a biodesulfurization technology where the production of the biocatalyst is one of the key topics. Traditionally, the production is carried out in batch culture where the maximum cellular concentration is restricted by inherent limitations of the culture type and the microorganism growth rate. This work addresses the production of two desulfurizing microorganisms: Rhodococcus erythropolis IGTS8 and gordona rubropertinctus ICP172 using fed-batch culture. Fed-batch cultures were conducted in a 12 L fermentor using ICP 4 medium containing glucose and DMSO as carbon and sulfur sources. As a result, cell concentration was increased 1.5 and 3 times with fed-batch cultures using constant and exponential flow respectively, achieving a maximum cell concentration of 7.3 g DCW/L of biocatalyst igts8 and 12.85 gGDCW/L of the new biocatalyst ICP172. Both biocatalysts presented biodesulfurization activity in a spiked matrix DBT/HXD and in diesel matrix with the detection of 2-HBP which is the end-product of DBT degradation pathway

  15. Experimental and mathematical model of the interactions in the mixed culture of links in the “producer-consumer” cycle

    Science.gov (United States)

    Pisman, T. I.

    2009-07-01

    The paper presents a experimental and mathematical model of interactions between invertebrates (the ciliates Paramecium caudatum and the rotifers Brachionus plicatilis) in the "producer-consumer" aquatic biotic cycle with spatially separated components. The model describes the dynamics of the mixed culture of ciliates and rotifers in the "consumer" component, feeding on the mixed algal culture of the "producer" component. It has been found that metabolites of the algae Scenedesmus produce an adverse effect on the reproduction of the ciliates P. caudatum. Taking into account this effect, the results of investigation of the mathematical model were in qualitative agreement with the experimental results. In the "producer-consumer" biotic cycle it was shown that coexistence is impossible in the mixed culture of invertebrates of the "consumer" component. The ciliates P. caudatum are driven out by the rotifers B. plicatilis.

  16. Finding meaning in first episode psychosis: experience, agency, and the cultural repertoire.

    Science.gov (United States)

    Larsen, John Aggergaard

    2004-12-01

    The article examines individuals' attempts to generate meaning following their experiences with psychosis. The inquiry is based on a person-centered ethnographic study of a Danish mental health community program for early intervention in schizophrenia and involves longitudinal interviews with 15 of its participants. The article takes an existential anthropological perspective emphasizing agency and cultural phenomenology to investigate how individuals draw on resources from the cultural repertoire to make sense of personally disturbing experiences during their psychosis. It is suggested that the concept of "system of explanation" has advantages over, for example, "illness narrative" and "explanatory model" when demonstrating how some individuals engage in the creative analytic and theory-building work of bricolage, selecting, adding, and combining various systems of explanation. Delusions are equally derived from the cultural repertoire but are constructed as dogmatic explanations that are idiosyncratic to the individual who holds them.

  17. In vitro oocyte culture and somatic cell nuclear transfer used to produce a live-born cloned goat.

    Science.gov (United States)

    Ohkoshi, Katsuhiro; Takahashi, Seiya; Koyama, Shin-Ichiro; Akagi, Satoshi; Adachi, Noritaka; Furusawa, Tadashi; Fujimoto, Jun-Ichiro; Takeda, Kumiko; Kubo, Masanori; Izaike, Yoshiaki; Tokunaga, Tomoyuki

    2003-01-01

    The use of an in vitro culture system was examined for production of somatic cells suitable for nuclear transfer in the goat. Goat cumulus-oocyte complexes were incubated in tissue culture medium TCM-199 supplemented with 10% fetal bovine serum (FBS) for 20 h. In vitro matured (IVM) oocytes were enucleated and used as karyoplast recipients. Donor cells obtained from the anterior pituitary of an adult male were introduced into the perivitelline space of enucleated IVM oocytes and fused by an electrical pulse. Reconstituted oocytes were cultured in chemically defined medium for 9 days. Two hundred and twenty-eight oocytes (70%) were fused with donor cells. After in vitro culture, seven somatic cell nuclear transfer (SCNT) oocytes (3%) developed to the blastocyst stage. SCNT embryos were transferred to the oviducts of recipient females (four 8-cell embryos per female) or uterine horn (two blastocysts per female). One male clone (NT1) was produced at day 153 from an SCNT blastocyst and died 16 days after birth. This study demonstrates that nuclear transferred goat oocytes produced using an in vitro culture system could develop to term and that donor anterior pituitary cells have the developmental potential to produce term offspring. In this study, it suggested that the artificial control of endocrine system in domestic animal might become possible by the genetic modification to anterior pituitary cells.

  18. EDUCATIONAL QUEST AS MEANS OF FORMING OF INFORMATION CULTURE

    Directory of Open Access Journals (Sweden)

    В И Глизбург

    2016-12-01

    Full Text Available Creative use of the potential of modern information and communication technologies for solving pedagogical, educational and training tasks is now an urgent problem of education. It is already impossible to isolate pupils from the modern means of communication (smartphones, tablets, etc.. The objective of a teacher to show how you can use the gadgets for educational purposes. Interest in their use as a source of useful information. Learn to evaluate and select information sources on various criteria: reliability, availability, adequacy and completeness of the information, response rate, etc. Theseand other skills to work with information are key in our rapidly changing world, and are part of the concept of information culture of the modern man. This article describes the use of educational technology of QR-codes to address these issues, as well as justification its effectiveness. The intellectual quest, which is the embodiment of the above ideas, combines the dynamism and kindness, interest and attentiveness, ability to work in a team and contribute to the common cause; aimed at the development of tolerance, inquisitiveness, activity, courtesy, accuracy, attention and thinking; as well as to broaden their horizons and the formation of information culture of schoolboys.

  19. THE MEANINGS OF DWELLING ATTRIBUTES FOR TEMPORARY RESIDENTS FROM DIFFERENT CULTURES: THE CASE OF KOREAN TEMPORARY RESIDENTS IN THE UNITED STATES

    Directory of Open Access Journals (Sweden)

    Eunsil Lee

    2010-03-01

    Full Text Available The cross-cultural temporary resident population is one of the fastest growing cultural groups in the United States. However, their housing experiences in the new environment have not been extensively studied. Thus, the current study sought to examine meanings of dwelling attributes for cross-cultural temporary residents in the host country. In order to obtain insights into not only functional meanings but also underlying values, a conceptual framework was developed based primarily on Gutman’s (1982 means-end theory and Rapoport’s (1988 three levels of meaning. A case study was conducted using indepth laddering interviews with ten Korean temporary residents in the Lansing, Michigan, area. Seven dwelling attributes emerged from interviews: two satisfactory attributes (i.e., surrounding natural environment and architecture and five unsatisfactory attributes (i.e., carpeted floor, interior lighting, acoustics, bathroom, and entryway. Data were analyzed utilizing the measurement of means-end chain (Gutman, 1982, identifying the lower-level, everyday meanings as well as middle-level, latent meanings of dwelling attributes. A hierarchical value map was used to illustrate the interrelationships among the attributes, consequences, and values. Results revealed that dwelling attributes in participants’ current housing did not effectively satisfy their fundamental needs. In particular, carpeted floor was linked to the greatest number of negative meanings. Moreover, the cultural aspects of Korean housing affected the meanings of dwelling attributes in participants’ current homes. Findings suggest design professionals, facility managers, and policymakers must understand how people from other cultures attach different meanings to the dwelling attributes in their homes and provide more culturally responsive residential environments.

  20. Ex Vivo Produced Oral Mucosa Equivalent by Using the Direct Explant Cell Culture Technique

    Directory of Open Access Journals (Sweden)

    Kamile Öztürk

    2012-09-01

    Full Text Available Objective: The aim of this study is the histological and immunohistochemical evaluation of ex vivo produced oral mucosal equivalents using keratinocytes cultured by direct explant technique.Material and Methods: Oral mucosa tissue samples were obtained from the keratinized gingival tissues of 14 healthy human subjects. Human oral mucosa keratinocytes from an oral mucosa biopsy specimen were dissociated by the explant technique. Once a sufficient population of keratinocytes was reached, they were seeded onto the type IV collagen coated “AlloDerm” and taken for histological and immunohistochemical examinations at 11 days postseeding of the keratinocytes on the cadaveric human dermal matrix.Results: Histopathologically and immunohistochemically, 12 out of 14 successful ex vivo produced oral mucosa equivalents (EVPOME that consisted of a stratified epidermis on a dermal matrix have been developed with keratinocytes cultured by the explant technique.Conclusion: The technical handling involved in the direct explant method at the beginning of the process has fewer steps than the enzymatic method and use of the direct explant technique protocol for culturing of human oral mucosa keratinocyte may be more adequate for EVPOME production.

  1. Prevalence of extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated from blood cultures in Africa.

    Science.gov (United States)

    Sangare, S A; Maiga, A I; Guindo, I; Maiga, A; Camara, N; Savadogo, S; Diallo, S; Bougoudogo, F; Armand-Lefevre, L; Andremont, A; Maiga, I I

    2015-09-01

    Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae have been isolated from many regions of the world. Epidemiological studies are being conducted in Europe, North America, and Asia. No study has however been conducted in Africa to determine the prevalence and distribution of ESBLs on the continent. This literature review aimed at describing the prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures, as well as the ESBL genes involved at the international level. Our focus was mainly on Africa. We conducted a literature review on PubMed. Articles related to our study field and published between 1996 and 2014 were reviewed and entirely read for most of them, while we only focused on the abstracts of some other articles. Relevant articles to our study were then carefully reviewed and included in the review. The prevalence of ESBL-producing Enterobacteriaceae differs from one country to another. The results of our literature review however indicate that class A ESBLs prevail over the other types. We took into consideration articles focusing on various types of samples to assess the prevalence of ESBL-producing Enterobacteriaceae, but information on isolates from blood cultures is limited. The worldwide prevalence of ESBL-producing Enterobacteriaceae has increased over time. Evidence of ESBL-producing Enterobacteriaceae can be found in all regions of the world. Studies conducted in Africa mainly focused on the Northern and Eastern parts of the continent, while only rare studies were carried out in the rest of the continent. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  2. The Cultural Meaning of Cardiac Illness and Self-Care Among Lebanese Patients With Coronary Artery Disease.

    Science.gov (United States)

    Dumit, Nuhad Yazbik; Magilvy, Joan Kathy; Afifi, Rima

    2016-07-01

    Cardiac disease is the leading cause of death in Lebanon, accounting for 22% to 26% of total deaths in the country. A thorough understanding of perceptions of cardiac illness and related self-care management is critical to the development of secondary prevention programs that are specific to the Lebanese culture. To explore the cultural perceptions of cardiac illness and the associated meaning of self-care among Lebanese patients. Using a qualitative descriptive method, semistructured interviews were conducted with a purposive sample of 15 Lebanese cardiac patients recruited from a medical center in Beirut, Lebanon. The qualitative descriptive analysis yielded one overarching and two other themes describing perceptions of cardiac illness and self-care within the Lebanese cultural context. The overarching cultural theme was, "Lebanese cardiac patients were unfamiliar with the term concept and meaning of self-care." Lebanese cardiac patients thanked God and accepted their fate (Theme I). The participants considered their cardiac incident a life or death warning (Theme II). Health care providers need to consider patients' cultural perception of illness while planning and evaluating cardiac self-care programs. © The Author(s) 2015.

  3. Detection of hydrogen peroxide-producing Lactobacillus species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women

    Directory of Open Access Journals (Sweden)

    Balkus Jennifer E

    2012-08-01

    Full Text Available Abstract Background The presence of hydrogen peroxide (H2O2 producing Lactobacillus in the vagina may play a role in controlling genital HIV-1 shedding. Sensitive molecular methods improve our ability to characterize the vaginal microbiota; however, they cannot characterize phenotype. We assessed the concordance of H2O2-producing Lactobacillus detected by culture with quantitative PCR (qPCR detection of Lactobacillus species commonly assumed to be H2O2-producers. Methods Samples were collected as part of a prospective cohort study of HIV-1 seropositive US women. Cervicovaginal lavage specimens were tested for L. crispatus and L. jensenii using 16S rRNA gene qPCR assays. Vaginal swabs were cultured for Lactobacillus and tested for H2O2-production. We calculated a kappa statistic to assess concordance between culture and qPCR. Results Culture and qPCR results were available for 376 visits from 57 women. Lactobacilli were detected by culture at 308 (82% visits, of which 233 of 308 (76% produced H2O2. L. crispatus and/or L. jensenii were detected at 215 (57% visits. Concordance between detection of L. crispatus and/or L. jensenii by qPCR and H2O2-producing Lactobacillus by culture was 75% (kappa = 0.45. Conclusions Among HIV-1 seropositive women, there was a moderate level of concordance between H2O2-producing Lactobacillus detected by culture and the presence of L. crispatus and/or L. jensenii by qPCR. However, one-quarter of samples with growth of H2O2-producing lactobacilli did not have L. crispatus or L. jensenii detected by qPCR. This discordance may be due to the presence of other H2O2-producing Lactobacillus species.

  4. Symbolic Meanings of High and Low Impact Daily Consumption Practices in Different Cultures

    DEFF Research Database (Denmark)

    Ger, Güliz; Wilhite, Harold; Halkier, Bente

    1998-01-01

    . They influence our choices and practices whether it is by motivating or impeding them. In this study we will look at the symbolic meanings of high and low environmental impact consumption practices. In order to create desirable social markers for environmentally-friendly practices and/or to change high impact...... practices, we need to understand emergent practices and their existing cultural meanings. Thus we have chosen three fields of daily consumption practices - food consumption, transport and hygiene - and sorted out the relatively environmentally friendly (low impact) and the relatively environmentally...

  5. Glycoprotein Ibα receptor instability is associated with loss of quality in platelets produced in culture.

    Science.gov (United States)

    Robert, Amélie; Boyer, Lucie; Pineault, Nicolas

    2011-03-01

    The development of culture processes for hematopoietic progenitors could lead to the development of a complementary source of platelets for therapeutic purposes. However, functional characterization of culture-derived platelets remains limited, which raises some uncertainties about the quality of platelets produced in vitro. The aim of this study was to define the proportion of functional platelets produced in cord blood CD34+ cell cultures. Toward this, the morphological and functional properties of culture-derived platelet-like particles (PLPs) were critically compared to that of blood platelets. Flow cytometry combined with transmission electron microscopy analyses revealed that PLPs formed a more heterogeneous population of platelets at a different stage of maturation than blood platelets. The majority of PLPs harbored the fibrinogen receptor αIIbβ3, but a significant proportion failed to maintain glycoprotein (GP)Ibα surface expression, a component of the vWF receptor essential for platelet functions. Importantly, GPIbα extracellular expression correlated closely with platelet function, as the GPIIb+ GPIbα+ PLP subfraction responded normally to agonist stimulation as evidenced by α-granule release, adhesion, spreading, and aggregation. In contrast, the GPIIb+ GPIbα⁻ subfraction was unresponsive in most functional assays and appeared to be metabolically inactive. The present study confirms that functional platelets can be generated in cord blood CD34+ cell cultures, though these are highly susceptible to ectodomain shedding of receptors associated with loss of function. Optimization of culture conditions to prevent these deleterious effects and to homogenize PLPs is necessary to improve the quality and yields of culture-derived platelets before they can be recognized as a suitable complementary source for therapeutic purposes.

  6. USING THE MEANS OF THEATRE ART TO DEVELOP MODERN TEACHERS’ RHETORICAL CULTURE

    Directory of Open Access Journals (Sweden)

    Dmytro Budianskyi

    2017-04-01

    Full Text Available The article examines an important problem of modern education – the use of theatre arts to improve the teachers’ oratorical skills. In the pedagogical context the main categories of theatre pedagogy (co-creation, reincarnation, tempo, rhythm, mise-en-scène, etc. are discussed in details. Thus the congruence of teaching and dramatic art is found out. The essence of artistry is determined as an element of a teacher’s rhetorical culture. The contents, structural elements of Stanislavsky system and their use for developing the teacher’s rhetorical culture are analyzed. The author discusses in details the two main components of Stanislavsky system: an actor’s work itself and the actor’s work on the role. It is proved that both parts of this system with a few adjustments correspond to the method of forming the teacher-speaker’s artistic and rhetorical qualities. The development of teacher’s external techniques includes the preparation of the bodily apparatus for teaching. According to the author’s point of view, a teacher like an actor should have the mental technique to be able to call creative feeling at the right time. Special attention is paid to the various types of artistic training, which is an effective tool for developing the teacher’s rhetorical culture. The system of training includes exercises to develop speech culture, plastic expression, emotional memory, behavior naturalness, etc. The article focuses on the need to study and use the main principles of Stanislavsky system in the teachers’ practical activities. The most effective means of creating a creative mood is physical activity. The author emphasizes the necessity of developing modern-teachers’ artistic and rhetorical qualities by means of theatre pedagogy with the aim of increasing the efficiency of the educational process. The results of the study can be used in the process of training, professional activities and advanced training of education workers.

  7. Chemical evaluation of strawberry plants produced by tissue culturing of gamma irradiated seedlings

    International Nuclear Information System (INIS)

    Maraei, R.W.

    2007-01-01

    studies were conducted to evaluate the influence of gamma irradiation as a supplementary factor precedes tissue culture application on strawberry seedlings (c.v.Rosa Linda). the strawberry seedling were irradiated using 8 doses of co 60 gamma rays 50.75.100.125 ,150,250, 350 and 500 gray. tissue culture technique was applied on irradiated and unirradiated strawberry seedling. different characteristics of plantlets, plant and fruit of strawberry produced from the double treatment (irradiation followed by tissue culture) were studied as well as the early, total and exportable fruit yields. data indicated that, low radiation doses 50,75 and 100 gray increased all morphological and chemical characteristics of the plantlets, plant and fruit of strawberry, whereas radiation doses higher than 100 gray decreased them significantly. moreover 350 and gray were lethal doses. radiation dose 50 gray increased the survival percentage and the length of plantlets by 1.5% and 50% respectively more than the unirradiated treatment in all multiplication stages

  8. Culture and Palliative Care: Preferences, Communication, Meaning, and Mutual Decision Making.

    Science.gov (United States)

    Cain, Cindy L; Surbone, Antonella; Elk, Ronit; Kagawa-Singer, Marjorie

    2018-05-01

    Palliative care is gaining acceptance across the world. However, even when palliative care resources exist, both the delivery and distribution of services too often are neither equitably nor acceptably provided to diverse population groups. The goal of this study was to illustrate tensions in the delivery of palliative care for diverse patient populations to help clinicians to improve care for all. We begin by defining and differentiating culture, race, and ethnicity, so that these terms-often used interchangeably-are not conflated and are more effectively used in caring for diverse populations. We then present examples from an integrative literature review of recent research on culture and palliative care to illustrate both how and why varied responses to pain and suffering occur in different patterns, focusing on four areas of palliative care: the formation of care preferences, communication patterns, different meanings of suffering, and decision-making processes about care. For each area, we provide international and multiethnic examples of variations that emphasize the need for personalization of care and the avoidance of stereotyping beliefs and practices without considering individual circumstances and life histories. We conclude with recommendations for improving palliative care research and practice with cultural perspectives, emphasizing the need to work in partnerships with patients, their family members, and communities to identify and negotiate culturally meaningful care, promote quality of life, and ensure the highest quality palliative care for all, both domestically and internationally. Copyright © 2018 American Academy of Hospice and Palliative Medicine. Published by Elsevier Inc. All rights reserved.

  9. Understanding Cross-Cultural Meaning through Visual Media.

    Science.gov (United States)

    Hedberg, John G.; Brown, Ian

    2002-01-01

    Discusses cultural differences in Web site design for cross-cultural contexts and describes a study of Masters Degree students in Hong Kong that investigated their perceptions of Web learning environments that had been designed in Australia and delivered into Hong Kong and China. Considers the appropriateness of western interface design…

  10. Temperature and pH optima of enzyme activities produced by cellulolytic thermophilic fungi in batch and solid-state cultures

    Energy Technology Data Exchange (ETDEWEB)

    Grajek, W

    1986-01-01

    The temperature and pH optima of cellulolytic activities produced by thermophilic fungi in liquid and solid-state cultures were established. Some differences in optimal conditions for enzyme activities, which depended on culture methods, were confirmed. 10 references.

  11. Biofilm formation by designed co-cultures of Caldicellulosiruptor species as a means to improve hydrogen productivity.

    Science.gov (United States)

    Pawar, Sudhanshu S; Vongkumpeang, Thitiwut; Grey, Carl; van Niel, Ed Wj

    2015-01-01

    Caldicellulosiruptor species have gained a reputation as being among the best microorganisms to produce hydrogen (H2) due to possession of a combination of appropriate features. However, due to their low volumetric H2 productivities (Q H2), Caldicellulosiruptor species cannot be considered for any viable biohydrogen production process yet. In this study, we evaluate biofilm forming potential of pure and co-cultures of Caldicellulosiruptor saccharolyticus and Caldicellulosiruptor owensensis in continuously stirred tank reactors (CSTR) and up-flow anaerobic (UA) reactors. We also evaluate biofilms as a means to retain biomass in the reactor and its influence on Q H2. Moreover, we explore the factors influencing the formation of biofilm. Co-cultures of C. saccharolyticus and C. owensensis form substantially more biofilm than formed by C. owensensis alone. Biofilms improved substrate conversion in both of the reactor systems, but improved the Q H2 only in the UA reactor. When grown in the presence of each other's culture supernatant, both C. saccharolyticus and C. owensensis were positively influenced on their individual growth and H2 production. Unlike the CSTR, UA reactors allowed retention of C. saccharolyticus and C. owensensis when subjected to very high substrate loading rates. In the UA reactor, maximum Q H2 (approximately 20 mmol · L(-1)  · h(-1)) was obtained only with granular sludge as the carrier material. In the CSTR, stirring negatively affected biofilm formation. Whereas, a clear correlation was observed between elevated (>40 μM) intracellular levels of the secondary messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) and biofilm formation. In co-cultures C. saccharolyticus fortified the trade of biofilm formation by C. owensensis, which was mediated by elevated levels of c-di-GMP in C. owensensis. These biofilms were effective in retaining biomass of both species in the reactor and improving Q H2 in a UA reactor using

  12. Meanings of health: interrogating structure and culture.

    Science.gov (United States)

    Dutta, Mohan Jyoti; Basu, Ambar

    2008-11-01

    Based on the argument that context ought to be centralized in discourses of health communication, this article applies the culture-centered approach to engage in dialogue about issues of health with 18 men in rural West Bengal. The culture-centered approach is based on dialogue between the researcher and the community members, with the goals of listening to the voices of cultural members in suggesting culture-based health solutions. In this project, our discursive engagement with the participants suggests that health is primarily constructed as an absence, framed in the realm of minimal access to healthcare resources. In a situation where the resources are limited, the participants discussed the importance of trust in their relationship with the local provider. Health was also seen as a collective resource that was both an asset of the collective and a responsibility of the collective. Finally, the participants also pointed out the ways in which corruption in the structure introduced a paradox in policy discourse and the material conditions of the participants.

  13. Producing health, producing safety. Developing a collective safety culture in radiotherapy

    International Nuclear Information System (INIS)

    Nascimento, Adelaide

    2009-01-01

    This research thesis aims at a better understanding of safety management in radiotherapy and at proposing improvements for patient safety through the development of a collective safety culture. A first part presents the current context in France and abroad, addresses the transposition of other safety methods to the medical domain, and discusses the peculiarities of radiotherapy in terms of risks and the existing quality-assurance approaches. The second part presents the theoretical framework by commenting the intellectual evolution with respect to system safety and the emergence of the concept of safety culture, and by presenting the labour collective aspects and their relationship with system safety. The author then comments the variety of safety cultures among the different professions present in radiotherapy, highlights the importance of the collective dimension in correcting discrepancies at the end of the treatment process, and highlights how physicians take their colleagues work into account. Recommendations are made to improve patient safety in radiotherapy

  14. Meanings of Care: A Culture-Centered Approach to Left-Behind Family Members in the Countryside of China.

    Science.gov (United States)

    Sun, Kang; Dutta, Mohan J

    2016-11-01

    Critical studies of health communication foreground the importance of meanings as organizing frameworks for constituting health. The contested and contradictory meanings articulated around health offer insights into the constraining and enabling roles of structures. Through ethnographic fieldwork conducted in a village in China embedded within the activist framework of the culture-centered approach, this project explores understandings of family care amid left-behind families in rural China against the backdrop of the migration of the middle generation of working adults from families located in rural contexts to cities. We work with the culture-centered approach to explore local meanings of care, understanding everyday care as integral to the health of an aging population that has been erased from the discursive space by market reforms and the hegemonic narrative of national development in China.

  15. Gas and Pressure Dependence for the Mean Size of Nanoparticles Produced by Laser Ablation of Flowing Aerosols

    International Nuclear Information System (INIS)

    Nichols, William T.; Malyavanatham, Gokul; Henneke, Dale E.; Brock, James R.; Becker, Michael F.; Keto, John W.; Glicksman, Howard D.

    2000-01-01

    Silver nanoparticles were produced by laser ablation of a continuously flowing aerosol of microparticles entrained in argon, nitrogen and helium at a variety of gas pressures. Nanoparticles produced in this new, high-volume nanoparticle production technique are compared with our earlier experiments using laser ablation of static microparticles. Transmission electron micrographs of the samples show the nanoparticles to be spherical and highly non-agglomerated under all conditions tested. These micrographs were analyzed to determine the effect of carrier gas type and pressure on size distributions. We conclude that mean diameters can be controlled from 4 to 20 nm by the choice of gas type and pressure. The smallest nanoparticles were produced in helium, with mean sizes increasing with increasing molecular weight of the carrier gas. These results are discussed in terms of a model based on cooling via collisional interaction of the nanoparticles, produced in the laser exploded microparticle, with the ambient gas

  16. The importance of socio-cultural context for understanding students' meaning making in the study of genetics

    Science.gov (United States)

    Furberg, Anniken; Arnseth, Hans Christian

    2009-03-01

    In this rejoinder to Ann Kindfield and Grady Venville's comments on our article "Reconsidering conceptual change from a socio-cultural perspective: Analyzing students' meaning making in genetics in collaborative learning activities," we elaborate on some of the critical issues they raise. Their comments make apparent some of the crucial differences between a socio-cultural and a socio-cognitive approach towards conceptual change. We have selected some issues that are addressed, either implicitly or explicitly, in their comments. The main issues discussed are talk and interaction as data, the significance of context in interaction studies, the feasibility of generic claims in small-scale interaction studies, and the difference between studying students' understanding of science concepts as opposed to studying the construction of meaning.

  17. Independent Learning Crossing Cultures: Learning Cultures and Shifting Meanings

    Science.gov (United States)

    Spiro, Jane; Henderson, Juliet; Clifford, Valerie

    2012-01-01

    This paper contrasts the notion of "independent learning" as perceived by two informant groups at a UK institution of higher education: (1) teachers, educators and providers of education and (2) their students or "consumers" of education. Both informant groups are staff and students studying in a culture different to that of…

  18. Teaching University Students Cultural Diversity by Means of Multi-Cultural Picture Books in Taiwan

    Science.gov (United States)

    Wu, Jia-Fen

    2017-01-01

    In a pluralistic society, learning about foreign cultures is an important goal in the kind of multi-cultural education that will lead to cultural competency. This study adopted a qualitative dominant mixed-method approach to examine the effectiveness of the multi-cultural picture books on: (1) students' achieving awareness towards cultural…

  19. Meaning in hoarding: perspectives of people who hoard on clutter, culture and agency.

    Science.gov (United States)

    Orr, David M R; Preston-Shoot, Michael; Braye, Suzy

    2017-12-12

    Hoarding has become increasingly prominent in clinical practice and popular culture in recent years, giving rise to extensive research and commentary. Critical responses in the social sciences have criticised the cultural assumptions built in to the construct of 'hoarding disorder' and expressed fears that it may generate stigma outweighing its benefits; however, few of these studies have engaged directly with 'hoarders' themselves. This paper reports on in-depth, semi-structured interviews with 10 individuals living in England, who received assessment and intervention for hoarding from Social Services. Their narratives drew on the cultural repertoire of values and discourses around waste and worth, the mediation of sociality and relationships through material objects, physical constraints on keeping order and the role played by mental health. Analysing these perspectives anthropologically shows how dominant models of hoarding, such as the DSM-5 paradigm, potentially lend themselves to reductionist understandings that efface the meaning 'hoarding' may have and thereby deny agency to the person labelled as 'hoarder'. More culturally informed analysis, by contrast, affords insights into the complex landscape of value, waste, social critique, emotion, interpersonal relationships and practical difficulties that may underlie hoarding cases, and points the way to more person-centred practice and analysis.

  20. Parental grief and memento mori photography: narrative, meaning, culture, and context.

    Science.gov (United States)

    Blood, Cybele; Cacciatore, Joanne

    2014-01-01

    Postmortem photography is a widespread practice in perinatal bereavement care, yet few studies have explored how it affects bereaved parents, or how it might be received by parents of older children. This study is an examination of the meaning, utility, and social context of postmortem photography in a sample of 181 bereaved parents. Data were subjected to both quantitative and qualitative analysis. Photographs were positively regarded by most parents after perinatal death and several parents of older children. Other parents rejected postmortem photography for aesthetic, personal, or cultural reasons. Brief recommendations are offered for healthcare providers.

  1. THE INFLUENCE OF LANGUAGE COMPETENCE, WRITING COMPETENCE, AND CULTURAL COMPETENCE ON PRODUCING A SUCCESSFUL WRITING

    Directory of Open Access Journals (Sweden)

    Hermanto Hermanto

    2008-11-01

    Full Text Available Writing is a skill derived from a long way of learning and exercises. Different from other language skills, writing is considered the difficult language skill to acquire since it involves many aspects of linguistics, social, and writing knowledge and conventions. There are at least three important elements of writing useful to produce a good piece of composition, language competence, writing competence and cultural competence. This paper shows the influence of these three elements in order to produce good, readable, communicative, and successful writing

  2. Rheology of spreadable goat cheese made with autochthonous lactic cultures differing in their ability to produce exopolysaccharides

    Directory of Open Access Journals (Sweden)

    Frau Silvia Florencia

    2013-06-01

    Full Text Available The aim of this study was to compare the rheology of spreadable cheeses elaborated with autochthonous lactic starter cultures without the addition of exopolysaccharide-producing strain in the same starter with exopolysaccharide-producing strain. From a rheological standpoint, both samples were characterized as weak viscoelastic gels and pseudoplastic products. It was concluded that cheese made with exopolysaccharide-producing strain showed smaller G', G", and η* values over the range of frequencies studied and smaller critic stress values than the cheese without exopolysaccharide-producing strain. The results obtained indicate that cheeses without exopolysaccharide-producing strain need to be added with any texture enhancer product.

  3. To Introduce a Cultural Sense in Translation——A Brief Study of Non-equivalence in the Associated Meanings of English and Chinese Words

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This paper attempts to demonstrate the importance of intrpdicomg cultural sense in translation practice,English to Chinese in particular,by examining the problem of non-equivalence at word level in associated meanings and to unveil the cultural aspects projected within.It is also argued that greater importance should be attached to the cultural perspectives.in translation and flexible strategies should be adopted in dealing with the problems of non-equivalence in the associated meanings of words of different cultures.

  4. Symbolic severance. Charlie or the broken circle of cultural mediation

    Directory of Open Access Journals (Sweden)

    Isabelle MATHIEU

    2015-07-01

    Full Text Available The cultural dimension of the attack on Charlie Hebdo raises questions about the meaning of the event in terms of public policies on art and culture. At issue particulary is the re-situation of those policies in their relationship with democratic achievement in the context of the French republican model. Analysis through the prism of cultural mediation, considered as a communication process designed ti produce a symbolic type of work, casts new light on the objectives and the ways and means of implementing such policies.

  5. Selective cultures for the isolation of biosurfactant producing bacteria: comparison of different combinations of environmental inocula and hydrophobic carbon sources.

    Science.gov (United States)

    Domingues, Patrícia M; Louvado, António; Oliveira, Vanessa; Coelho, Francisco J C R; Almeida, Adelaide; Gomes, Newton C M; Cunha, Angela

    2013-01-01

    The potential of estuarine microniches as reservoirs of biosurfactant-producing bacteria was evaluated by testing different combinations of inocula and hydrophobic carbon sources. Selective cultures using diesel, petroleum, or paraffin as hydrophobic carbon sources were prepared and inoculated with water from the surface microlayer, bulk sediments, and sediment of the rhizosphere of Halimione portulacoides. These inocula were compared regarding the frequency of biosurfactant-producing strains among selected isolates. The community structure of the selective cultures was profiled using denaturing gradient gel electrophoresis (DGGE) of the 16S rRNA gene fragments at the end of the incubation. The DGGE profiles corresponding to the communities established in selective cultures at the end of the incubation revealed that communities were different in terms of structural diversity. The highest diversity was observed in the selective cultures containing paraffin (H (') = 2.5). Isolates were obtained from the selective cultures (66) and tested for biosurfactant production by the atomized oil assay. Biosurfactant production was detected in 17 isolates identified as Microbacterium, Pseudomonas, Rhodococcus, and Serratia. The combination of estuarine surface microlayer (SML) water as inoculum and diesel as carbon source seems promising for the isolation of surfactant-producing bacteria. Supplemental materials are available for this article. Go to the publisher's online edition of Preparative Biochemistry and Biotechnology to view the supplemental file.

  6. Use of a solid-phase 3H-radioimmunoassay for the measurement of immunoglobulin produced in short-term cultures of antibody-secreting cells

    International Nuclear Information System (INIS)

    Mongini, P.K.A.; Heber-Katz, E.

    1982-01-01

    A solid-phase radioimmunoassay (RIA) for assaying immunoglobulin produced from antibody-secreting myeloma, hybridoma and immune spleen cells is described. Specific antibody is detected by culturing antibody-producing cells on antigen-coated flexible polyvinylchloride microtiter wells, washing away the cells, and measuring the bound specific antibody with tritiated affinity-purified anti-isotype reagents. Antibody produced from 10 3 myeloma cells can be detected with as little as 4 h of incubation. With 24-48 h of incubation, antibody from as few as approximately 3-15 myeloma, hybridoma or immune spleen plaque-forming cells (PFC) can be detected. This culture-well RIA has certain distinct advantages over the hemolytic PFC assay and RIA assays in which antibody in culture supernatants is measured. (Auth.)

  7. Formation of Pedagogical System for Individual Self-Development by Means of Physical Culture and Sport

    Science.gov (United States)

    Panachev, Valery D.; Zelenin, Leonid A.; Opletin, Anatoly A.; Verbytskyi, Sergei A.

    2017-01-01

    Problems of formation, development and introduction of the modern pedagogical selfdevelopment system in university educational process by means of physical culture and sport have been considered in this article. Such generated pedagogical system reflects practical implementation of social order on the modern educational paradigm aimed at creation…

  8. Functional redundancy ensures performance robustness in 3-stage PHA-producing mixed cultures under variable feed operation.

    Science.gov (United States)

    Carvalho, Gilda; Pedras, Inês; Karst, Soren M; Oliveira, Catarina S S; Duque, Anouk F; Nielsen, Per H; Reis, Maria A M

    2018-01-25

    Polyhydroxyalkanoates (PHA) are biopolymers that can be produced by mixed microbial cultures using wastes or industrial by-products, which represent an economical and environmental advantage over pure culture processes. The use of alternate feedstocks enables using seasonal by-products, providing that the process is resilient to transient conditions. The mixed microbial communities of a 3-stage PHA producing system fed initially with molasses and then cheese whey were investigated through amplicon sequencing of the 16S rRNA gene. The transition in feedstock resulted in an adaptation of the acidogenic community, where Actinobacteria dominated with sugarcane molasses (up to 93% of the operational taxonomic units) and Firmicutes, with cheese whey (up to 97%). The resulting fermentation products profile also changed, with a higher fraction of HV precursors obtained with molasses than cheese whey (7.1±0.5 and 1.7±0.7 gCOD/L, respectively). As for the PHA storing culture, the genera Azoarcus, Thauera and Paracoccus were enriched with fermented molasses (average 89% of Bacteria). Later, fermented cheese whey fostered a higher diversity, including some less characterised PHA-storers such as the genera Paenibacillus and Lysinibacillus. Although the microbial community structure was significantly affected by the feedstock shift, the acidogenic and PHA storing performance of the 3-stage system was very similar once a pseudo steady state was attained, showing that a reliable level of functional redundancy was attained in both mixed cultures. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Do you see what I mean? Corticospinal excitability during observation of culture-specific gestures.

    Directory of Open Access Journals (Sweden)

    Istvan Molnar-Szakacs

    2007-07-01

    Full Text Available People all over the world use their hands to communicate expressively. Autonomous gestures, also known as emblems, are highly social in nature, and convey conventionalized meaning without accompanying speech. To study the neural bases of cross-cultural social communication, we used single pulse transcranial magnetic stimulation (TMS to measure corticospinal excitability (CSE during observation of culture-specific emblems. Foreign Nicaraguan and familiar American emblems as well as meaningless control gestures were performed by both a Euro-American and a Nicaraguan actor. Euro-American participants demonstrated higher CSE during observation of the American compared to the Nicaraguan actor. This motor resonance phenomenon may reflect ethnic and cultural ingroup familiarity effects. However, participants also demonstrated a nearly significant (p = 0.053 actor by emblem interaction whereby both Nicaraguan and American emblems performed by the American actor elicited similar CSE, whereas Nicaraguan emblems performed by the Nicaraguan actor yielded higher CSE than American emblems. The latter result cannot be interpreted simply as an effect of ethnic ingroup familiarity. Thus, a likely explanation of these findings is that motor resonance is modulated by interacting biological and cultural factors.

  10. Analysis of microbial community variation during the mixed culture fermentation of agricultural peel wastes to produce lactic acid.

    Science.gov (United States)

    Liang, Shaobo; Gliniewicz, Karol; Gerritsen, Alida T; McDonald, Armando G

    2016-05-01

    Mixed cultures fermentation can be used to convert organic wastes into various chemicals and fuels. This study examined the fermentation performance of four batch reactors fed with different agricultural (orange, banana, and potato (mechanical and steam)) peel wastes using mixed cultures, and monitored the interval variation of reactor microbial communities with 16S rRNA genes using Illumina sequencing. All four reactors produced similar chemical profile with lactic acid (LA) as dominant compound. Acetic acid and ethanol were also observed with small fractions. The Illumina sequencing results revealed the diversity of microbial community decreased during fermentation and a community of largely lactic acid producing bacteria dominated by species of Lactobacillus developed. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Neuropharmacological and neuroprotective activities of some metabolites produced by cell suspension culture of Waltheria americana Linn.

    Science.gov (United States)

    Mundo, Jorge; Villeda-Hernández, Juana; Herrera-Ruiz, Maribel; Gutiérrez, María Del Carmen; Arellano-García, Jesús; León-Rivera, Ismael; Perea-Arango, Irene

    2017-10-01

    Waltheria americana is a plant used in Mexican traditional medicine to treat some nervous system disorders. The aims of the present study were to isolate and determine the neuropharmacological and neurprotective activities of metabolites produced by a cell suspension culture of Waltheria americana. Submerged cultivation of W. americana cells provided biomass. A methanol-soluble extract (WAsc) was obtained from biomass. WAsc was fractionated yielding the chromatographic fractions 4WAsc-H 2 O and WAsc-CH 2 Cl 2 . For the determination of anticonvulsant activity in vivo, seizures were induced in mice by pentylenetetrazol (PTZ). Neuropharmacological activities (release of gamma amino butyric acid (GABA) and neuroprotection) of chromatographic fractions were determined by in vitro histological analysis of brain sections of mice post mortem. Fraction 4WAsc-H 2 O (containing saccharides) did not produce neuronal damage, neurodegeneration, interstitial tissue edema, astrocytic activation, nor cell death. Pretreatment of animals with 4WAsc-H 2 O and WAsc-CH 2 Cl 2 from W. americana cell suspensions induced an increase in: GABA release, seizure latency, survival time, neuroprotection, and a decrease in the degree of severity of tonic/tonic-clonic convulsions, preventing PTZ-induced death of up to 100% of animals of study. Bioactive compounds produced in suspension cell culture of W. americana produce neuroprotective and neuropharmacological activities associated with the GABAergic neurotransmission system. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  12. Representing and Coping with Early Twentieth-Century Chongqing: “Guide Songs” as Maps, Memory Cells, and Means of Creating Cultural Imagery

    Directory of Open Access Journals (Sweden)

    Igor Chabrowski

    2013-03-01

    Full Text Available Chongqing’s “guide songs” form an interesting subgenre among the broad category of haozi 號子 (workers’ songs. These early twentieth-century songs were a form of rhythm-based oral narrative describing Chongqing’s urban spaces, river docks, and harbors. Each toponym mentioned in the lyrics was followed by a depiction of the characteristic associations, whether visible or symbolic, of the place. This article aims to analyze the verbal images of Chongqing presented in these songs in order to understand how the city was remembered, reproduced, and represented. The article deconstructs representations of the city produced by the lower classes, mainly by Sichuan boatmen, and links culturally meaningful images of urban spaces with the historical experiences of work, religion, and historical-mythical memory. It also points to the functions that oral narratives had in the urban environment of early twentieth-century Chongqing. Rhythmic and easy to remember, the songs provided ready-to-use guides and repositories of knowledge useful to anyone living or working there. A cross between utilitarian resource books and cultural representations, they shaped modes of thinking and visualizations of urban spaces and Chongqing. Finally, this article responds to the need to employ popular culture in our thinking about Chinese cities and the multiplicity of meanings they were given in pre-Communist times.

  13. Development and Use of a Real-Time Quantitative PCR Method for Detecting and Quantifying Equol-Producing Bacteria in Human Faecal Samples and Slurry Cultures

    Directory of Open Access Journals (Sweden)

    Lucía Vázquez

    2017-06-01

    Full Text Available This work introduces a novel real-time quantitative PCR (qPCR protocol for detecting and quantifying equol-producing bacteria. To this end, two sets of primers targeting the dihydrodaidzein reductase (ddr and tetrahydrodaidzein reductase (tdr genes, which are involved in the synthesis of equol, were designed. The primers showed high specificity and sensitivity when used to examine DNA from control bacteria, such as Slackia isoflavoniconvertens, Slackia equolifaciens, Asaccharobacter celatus, Adlercreutzia equolifaciens, and Enterorhabdus mucosicola. To demonstrate the validity and reliability of the protocol, it was used to detect and quantify equol-producing bacteria in human faecal samples and their derived slurry cultures. These samples were provided by 18 menopausal women under treatment of menopause symptoms with a soy isoflavone concentrate, among whom three were known to be equol-producers given the prior detection of the molecule in their urine. The tdr gene was detected in the faeces of all these equol-producing women at about 4–5 log10 copies per gram of faeces. In contrast, the ddr gene was only amplified in the faecal samples of two of these three women, suggesting the presence in the non-amplified sample of reductase genes unrelated to those known to be involved in equol formation and used for primer design in this study. When tdr and ddr were present in the same sample, similar copy numbers of the two genes were recorded. However, no significant increase in the copy number of equol-related genes along isoflavone treatment was observed. Surprisingly, positive amplification for both tdr and ddr genes was obtained in faecal samples and derived slurry cultures from two non-equol producing women, suggesting the genes could be non-functional or the daidzein metabolized to other compounds in samples from these two women. This novel qPCR tool provides a technique for monitoring gut microbes that produce equol in humans. Monitoring equol-producing

  14. Strontium ranelate changes the composition and crystal structure of the biological bone-like apatite produced in osteoblast cell cultures.

    Science.gov (United States)

    Querido, William; Campos, Andrea P C; Martins Ferreira, Erlon H; San Gil, Rosane A S; Rossi, Alexandre M; Farina, Marcos

    2014-09-01

    We evaluate the effects of strontium ranelate on the composition and crystal structure of the biological bone-like apatite produced in osteoblast cell cultures, a system that gave us the advantage of obtaining mineral samples produced exclusively during treatment. Cells were treated with strontium ranelate at concentrations of 0.05 and 0.5 mM Sr(2+). Mineral substances were isolated and analyzed by using a combination of methods: Fourier transform infrared spectroscopy, solid-state (1)H nuclear magnetic resonance, X-ray diffraction, micro-Raman spectroscopy and energy dispersive X-ray spectroscopy. The minerals produced in all cell cultures were typical bone-like apatites. No changes occurred in the local structural order or crystal size of the minerals. However, we noticed several relevant changes in the mineral produced under 0.5 mM Sr(2+): (1) increase in type-B CO3 (2-) substitutions, which often lead to the creation of vacancies in Ca(2+) and OH(-) sites; (2) incorporation of Sr(2+) by substituting slightly less than 10 % of Ca(2+) in the apatite crystal lattice, resulting in an increase in both lattice parameters a and c; (3) change in the PO4 (3-) environments, possibly because of the expansion of the lattice; (4) the Ca/P ratio of this mineral was reduced, but its (Ca+Sr)/P ratio was the same as that of the control, indicating that its overall cation/P ratio was preserved. Thus, strontium ranelate changes the composition and crystal structure of the biological bone-like apatite produced in osteoblast cell cultures.

  15. Hearsay Ethnography: Conversational Journals as a Method for Studying Culture in Action.

    Science.gov (United States)

    Watkins, Susan Cotts; Swidler, Ann

    2009-04-01

    Social scientists have long struggled to develop methods adequate to their theoretical understanding of meaning as collective and dynamic. While culture is widely understood as an emergent property of collectivities, the methods we use keep pulling us back towards interview-situated accounts and an image of culture as located in individual experience. Scholars who seek to access supra-individual semiotic structures by studying public rituals and other collectively-produced texts then have difficulty capturing the dynamic processes through which such meanings are created and changed in situ. To try to capture more effectively the way meaning is produced and re-produced in everyday life, we focus here on conversational interactions-the voices and actions that constitute the relational space among actors. Conversational journals provide us with a method: the analysis of texts produced by cultural insiders who keep journals of who-said-what-to-whom in conversations they overhear or events they participate in during the course of their daily lives. We describe the method, distinguishing it from other approaches and noting its drawbacks. We then illustrate the methodological advantages of conversational journals with examples from our texts. We end with a discussion of the method's potential in our setting as well as in other places and times.

  16. Understanding asexual identity as a means to facilitate culturally competent care: A systematic literature review.

    Science.gov (United States)

    Jones, Catriona; Hayter, Mark; Jomeen, Julie

    2017-12-01

    To provide a contemporary overview of asexuality and the implications this has for healthcare practice. Individuals belonging to sexual minority groups face many barriers in accessing appropriate health care. The term "sexual minority group" is usually used to refer to lesbian women, gay, bisexual and transgender individuals. Anecdotal and research evidence suggests that those who identify as asexual have similar poor experiences. Systematic review and qualitative analysis. This work uses a systematic review and qualitative analysis of the existing interview data from self-identified asexuals, to construct features of the asexual identity. The findings will help practitioners and health professionals develop an understanding of this poorly understood construct. Ultimately this work is aimed at facilitating culturally competent care in the context of asexuality. Qualitative analysis produced three themes, which can be used, not only to frame asexuality in a positive and normalising way, but also to provide greater understanding of asexuality, "romantic differences coupled with sexual indifference," "validation through engagement with asexual communities" and "a diversity of subasexual identities." Having some understanding of what it means to identify as asexual, and respecting the choices made by asexuals can markedly improve the experiences of those who embrace an asexual identity when engaging with health care. Anecdotal evidence, taken from one of the largest asexual online forums, suggests that a number of self-identified asexuals choose not to disclose their identity to healthcare professionals through fear of their asexual status being pathologised, problematised or judged. Given that asexuality is a poorly understood concept, this may be due to lack of understanding on behalf of healthcare providers. The review provides health professionals and practitioners working in clinical settings with some insights of the features of an asexual identity to facilitate

  17. Developing Psychological Culture of Schoolchildren as a Means of Supporting Implementation of Basic Education Standards

    Directory of Open Access Journals (Sweden)

    Dubrovina I.V.

    2018-01-01

    Full Text Available The paper reviews the social situation of development of children and adolescents in the modern society marked by rapid changes. The development of children and adolescents is described as ‘embedding into the culture’ through the education and is closely associated with the formation of their psychological culture. The paper analyses the conditions of personality development in modern children and adolescents, the factors which impede the communication and understanding of other people; it highlights the risks of escaping into the virtual reality or joining asocial groups. The paper also suggests important measures aimed at the formation of psychological culture in children in relation to age-specific tasks of development in primary school and adolescent ages. The development of psychological culture is regarded as the key means of supporting the implementation of modern educational standards as well as the foundation of psychological health in schoolchildren.

  18. Building a Strong Culture That Produces Sustainable Performance - 13444

    International Nuclear Information System (INIS)

    McDonald, John A. Jr

    2013-01-01

    Washington River Protection Solutions LLC (WRPS) has been involved with culture improvement for a number of years which has included co-chairing the industry effort to develop the EFCOG safety culture guidance documents [1, 2], and integration of this guidance into organizational processes and behavior expectations, described in more detail below. As various organizational cultural assessments have been periodically performed, and subsequent actions implemented to address improvement opportunities, organizational performance has shown improvement. Culture improvement is evident in the company's industrial safety statistics, event rates, safety culture survey results, employee morale, productivity, leadership effectiveness, and employee engagement. There does appear to be a relationship between striving to demonstrate behaviors consistent with excellent safety culture and good organizational performance over the past couple of years at WRPS. As performance continues to be evaluated, an improvement opportunity was identified to further enhance performance through field oriented behavioral/cultural improvement activities. WRPS recently conducted a three month effort to improve consistent implementation of management expectations by increasing management field presence with a focus on interacting real-time with workers and first line supervisors, and changing behaviors as appropriate. (authors)

  19. Building a Strong Culture That Produces Sustainable Performance - 13444

    Energy Technology Data Exchange (ETDEWEB)

    McDonald, John A. Jr [Washington River Protection Solutions LLC, PO Box 850, MSIN R2-53, Richland, WA 99352 (United States)

    2013-07-01

    Washington River Protection Solutions LLC (WRPS) has been involved with culture improvement for a number of years which has included co-chairing the industry effort to develop the EFCOG safety culture guidance documents [1, 2], and integration of this guidance into organizational processes and behavior expectations, described in more detail below. As various organizational cultural assessments have been periodically performed, and subsequent actions implemented to address improvement opportunities, organizational performance has shown improvement. Culture improvement is evident in the company's industrial safety statistics, event rates, safety culture survey results, employee morale, productivity, leadership effectiveness, and employee engagement. There does appear to be a relationship between striving to demonstrate behaviors consistent with excellent safety culture and good organizational performance over the past couple of years at WRPS. As performance continues to be evaluated, an improvement opportunity was identified to further enhance performance through field oriented behavioral/cultural improvement activities. WRPS recently conducted a three month effort to improve consistent implementation of management expectations by increasing management field presence with a focus on interacting real-time with workers and first line supervisors, and changing behaviors as appropriate. (authors)

  20. Sorry, We Didn't Mean to Break Your Culture

    Science.gov (United States)

    Lightfoot, R. C.

    Cultures meet and apparently insignificant traits or objects disrupt one of the cultures. This process repeatedly occurred in history with devastating consequences for the affected groups. Personal health, sanity, societies and cultures suffered from the effects of the new intrusions. Insights from sociological theory shed light on the effects and suggest remedies for at least some of the dysfunctions. Strategies for understanding and handling unforeseen changes must be prepared for future contact with other intelligences.

  1. VISUAL CULTURE AS A MEANS OF FORMING A COMMON AND PROFESSIONAL CULTURE OF A PERSON

    Directory of Open Access Journals (Sweden)

    N. V. Syrova

    2018-01-01

    Full Text Available Introduction. The article is devoted to the analysis of the visual culture, which forms the general and professional culture of a person in many ways. Attention is paid to the culture of perception of visual images, the ability to analyze them, evaluate, compare, also the features of visual thinking and creative activity, directly relating to it, are considered. The mastering of visual culture is grounded by the specificity of various creative specialties and is a component of the educational process.Materials and Methods. The theory of personality formation and development is the methodological basis of the article. During the research, the analysis of the scientific and theoretical concept of "visual culture" in the system of general and professional human culture was used.Results. The article illustrates that the artistic vision, developed in the process of visual culture formation, starting from childhood, actively participates in the preparation of the ground for the origin of an artistic image, which is realized in the material later. With the development of the creative imagination of the learner, also the development of thinking and memory takes place inevitably, that is harmonization of all parts of the brain is carried out, and this process must be taken into account in the upbringing and education, the formation of the personality, and the formed visual culture is the basis for the improvement of all verges of the student's creative abilities.Discussion and Conclusions. The conclusion is made that visual culture being an integral part of the educational process is the necessary condition for entering the sociocultural space. The education of a high level of visual culture in a modern information and technically replete society is one of the necessary components of the formation of a general and professional culture of a person and it is desirable to begin this process with a student, and even better, from school-days.

  2. Cultural Interpretations of the Visual Meaning of Icons and Images Used in North American Web Design

    Science.gov (United States)

    Knight, Eliot; Gunawardena, Charlotte N.; Aydin, Cengiz Hakan

    2009-01-01

    This study examines cross-cultural interpretations of icons and images drawn from US academic websites. Participants from Morocco, Sri Lanka, Turkey, and the USA responded to an online questionnaire containing 18 icons and images representing online functions and information types common on US academic websites. Participants supplied meanings for…

  3. The determination of the hadron mean free path for particle-producing collisions in intranuclear matter by measurement

    International Nuclear Information System (INIS)

    Strugalski, Z.; Mousa, M.

    1987-01-01

    It is shown how it is possible to determine the hadron mean free path in > for particle-producing collisions in intranuclear matter by measurement. The mean free path for the collisions of pions inside 54 131 Xe nuclei at 3.5 GeV/c momentum has been measured. The relation between in > in units nucleons/S and the hadron-nucleon inelastic cross section σ in in units S/nucleon is found: in >k1/σ in , where S∼10 fm 2 , k=3.0±0.15; physical meaning of S is given in this paper

  4. Stochastic modelling of Listeria monocytogenes single cell growth in cottage cheese with mesophilic lactic acid bacteria from aroma producing cultures

    DEFF Research Database (Denmark)

    Østergaard, Nina Bjerre; Christiansen, Lasse Engbo; Dalgaard, Paw

    2015-01-01

    . 2014. Modelling the effect of lactic acid bacteria from starter- and aroma culture on growth of Listeria monocytogenes in cottage cheese. International Journal of Food Microbiology. 188, 15-25]. Growth of L. monocytogenes single cells, using lag time distributions corresponding to three different......A stochastic model was developed for simultaneous growth of low numbers of Listeria monocytogenes and populations of lactic acid bacteria from the aroma producing cultures applied in cottage cheese. During more than two years, different batches of cottage cheese with aroma culture were analysed...

  5. Response to Richard Widdess: Music, Meaning and Culture

    Directory of Open Access Journals (Sweden)

    Jerome Lewis

    2012-09-01

    Full Text Available This commentary discusses the anthropological implications of Richard Widess’ paper by summarizing some anthropological approaches to music, especially focusing on the way musical participation inculcates and transmits an aesthetic orientation that guides action across cultural domains such as politics, economics and religion. The paper ends by suggesting that the heart of human culture is more likely to be an aesthetic orientation than a script or set of rules, and traces out some reasons why music does this so well.

  6. Oral fibroblasts produce more HGF and KGF than skin fibroblasts in response to co-culture with keratinocytes

    DEFF Research Database (Denmark)

    Grøn, Birgitte; Stoltze, Kaj; Andersson, Anders

    2002-01-01

    The production of hepatocyte growth factor (HGF) and keratinocyte growth factor (KGF) in subepithelial fibroblasts from buccal mucosa, periodontal ligament, and skin was determined after co-culture with keratinocytes. The purpose was to detect differences between the fibroblast subpopulations...... days by ELISA. When cultured on polystyrene, the constitutive level of KGF and HGF in periodontal fibroblasts was higher than the level in buccal and skin fibroblasts. In the presence of keratinocytes, all three types of fibroblasts in general increased their HGF and KGF production 2-3 times. When...... cells were maintained in collagen, the level of HGF and KGF was decreased mainly in skin cultures. However, in oral fibroblasts, induction after stimulation was at a similar level in collagen compared to on polystyrene. Skin fibroblasts maintained in collagen produced almost no HGF whether...

  7. Significados culturais da asma infantil Cultural meanings of the infantile asthma

    Directory of Open Access Journals (Sweden)

    Mirna Albuquerque Frota

    2008-06-01

    Full Text Available OBJETIVO: Analisar os significados culturais da asma infantil com origem na mãe-cuidadora. PROCEDIMENTOS METODOLÓGICOS: Pesquisa qualitativa realizada em 2004, no município de Fortaleza, CE. Foi utilizada a observação participante com abordagem etnográfica e entrevistas com sete mães acompanhantes de seus filhos em unidade de emergência hospitalar. Os significados das falas das mães foram identificados utilizando-se a técnica de análise temática. ANÁLISE DOS RESULTADOS: Foram identificadas duas categorias de discussão; na primeira, "desinformação sobre a doença", foi possível perceber que as mães não são informadas sobre a doença do filho. Na segunda categoria, "cuidado cultural", as mães referem informações sobre os cuidados e utilizam recursos do saber popular para prevenir a asma dos filhos, como o cuidado ambiental e a utilização de remédios caseiros à base de plantas medicinais. CONCLUSÕES: As características de desinformação e desconhecimento materno em relação à asma do filho mostram a necessidade de haver um trabalho educativo intenso, dialógico e problematizador em estreita colaboração com o tratamento, visando à melhoria do prognóstico da doença.OBJECTIVE: To analyze the cultural meanings of infantile asthma from the perspective of the mother/carer of the child. METHODOLOGICAL PROCEDURES: Qualitative research conducted in 2004, in the city of Fortaleza (Northeastern Brazil. An ethnographic approach was utilized, consisting of participant observation and interviews with seven mothers, accompanying their children in a hospital emergency ward. Thematic analysis was the technique employed when identifying the meanings of mothers' discourse. ANALYSIS OF RESULTS: Two discussion categories were identified: "disinformation on illness" in which it was possible to perceive that the mothers were not informed with respect to their children's illness; and "cultural care" in which they relate information on

  8. Lipolytic Changes in Fermented Sausages Produced with Turkey Meat: Effects of Starter Culture and Heat Treatment

    OpenAIRE

    Karslo?lu, Bet?l; ?i?ek, ?mran Ensoy; Kolsarici, Nuray; Cando?an, Kezban

    2014-01-01

    In this study, the effects of two different commercial starter culture mixes and processing methodologies (traditional and heat process) on the lipolytic changes of fermented sausages manufactured with turkey meat were evaluated during processing stages and storage. Free fatty acid (FFA) value increased with fermentation and during storage over 120 d in all fermented sausage groups produced with both processing methodologies (p

  9. Comparison of Sensory Properties, Shelf-Life and Microbiological Safety of Industrial Sausages Produced with Autochthonous and Commercial Starter Cultures

    Directory of Open Access Journals (Sweden)

    Jadranka Frece

    2014-01-01

    Full Text Available The aim of this research is to use isolated and characterized autochthonous functional starter cultures from traditional Croatian dry sausages and to evaluate their capacity for industrial production of five sausages (Čajna sausage, Zimska sausage, Bečka sausage, Srijemska sausage and Slavonski kulen. These defined autochthonous functional starter cultures (combination of Lactobacillus and Staphylococcus strains were used to produce five different industrial sausages which were compared by a panel. The viability of introduced autochthonous Lactobacillus and Staphylococcus strains and their effect on the final product characteristics, namely microbiological, physicochemical and sensory properties were monitored. The obtained results indicate that autochthonous starter cultures survived industrial production of sausages and can be used for production of sausages under controlled conditions. Autochthonous starter cultures obtained better results in the organoleptic evaluation, microbial safety and prolonged shelf-life in comparison with commercial starter cultures.

  10. Influencing agent group behavior by adjusting cultural trait values.

    Science.gov (United States)

    Tuli, Gaurav; Hexmoor, Henry

    2010-10-01

    Social reasoning and norms among individuals that share cultural traits are largely fashioned by those traits. We have explored predominant sociological and cultural traits. We offer a methodology for parametrically adjusting relevant traits. This exploratory study heralds a capability to deliberately tune cultural group traits in order to produce a desired group behavior. To validate our methodology, we implemented a prototypical-agent-based simulated test bed for demonstrating an exemplar from intelligence, surveillance, and reconnaissance scenario. A group of simulated agents traverses a hostile territory while a user adjusts their cultural group trait settings. Group and individual utilities are dynamically observed against parametric values for the selected traits. Uncertainty avoidance index and individualism are the cultural traits we examined in depth. Upon the user's training of the correspondence between cultural values and system utilities, users deliberately produce the desired system utilities by issuing changes to trait. Specific cultural traits are without meaning outside of their context. Efficacy and timely application of traits in a given context do yield desirable results. This paper heralds a path for the control of large systems via parametric cultural adjustments.

  11. Determination of cosmic ray produced radionuclides by means of background radiation counting system, 3

    International Nuclear Information System (INIS)

    1976-01-01

    This is the third report of the progress report series on studies of cosmic ray produced radionuclides by means of low background radiation counting system. In Part I some characteristics of a low beta-gamma coincidence spectrometer are described -- counter system, electronics, background spectra, counting efficiencies -- and studies on radioactive impurities in materials for scientific research are also described. In Part II, suitable solvents for a large scale liquid scintillation counter were examined and best combinations of solvents, solutes and naphthalene are shown. In Part III, miscellaneous topics are reported. (auth.)

  12. Formation of pedagogical system for individual selfdevelopment by means of physical culture and sport

    Directory of Open Access Journals (Sweden)

    Valery Panachev

    2017-11-01

    Full Text Available Problems of formation, development and introduction of the modern pedagogical selfdevelopment system in university educational process by means of physical culture and sport have been considered in this article. Such generated pedagogical system reflects practical implementation of social order on the modern educational paradigm aimed at creation of competitive, physically and morally strong individuals. This system promotes selfrealization of students’ individuality in formation of physical culture and sport competencies as well as competencies of selfdevelopment. Contemporary conditions of society’s development and analysis of the world social cultural and educational tendencies show that recently the object of scrupulous society’s attention and the subject of interdisciplinary research have become different aspects of person’s behavior in respect of his health in many highly developed countries. The slogan of such relation is: “Health is not everything but everything without health is nothing”. And this very principle specifies the problems of students’ selfdevelopment during intensive preparation for professional activity in the course of university training. These problems are aimed at maintenance of their health and improvement of physical preparation considering formation of professional motor competences for effective and qualitative mastering of future profession and career after graduation from the university.

  13. BIOCHEMICAL CHARACTERISTICS OF LACTIC ACID PRODUCING BACTERIA AND PREPARATION OF CAMEL MILK CHEESE BY USING STARTER CULTURE

    Directory of Open Access Journals (Sweden)

    T. Ahmed and R. Kanwal

    2004-04-01

    Full Text Available Lactic acid bacteria (LAB were isolated from camel milk by culturing the milk on specific media and pure culture was obtained by sub-culturing. Purification of culture was confirmed by Gram’s staining and identified by different biochemical tests. Camel milk contained lactic acid producing bacteria like Streptococci such as S. cremoris and S. lactis and Lactobacilli such as L. acidophilus. L. acidophilus grew more rapidly in camel milk than others as its growth was supported by camel milk. Ability of each strain was tested to convert lactose of milk into lactic acid. It was observed that 66% lactose was converted by S. lactis 20, whereas S. cremoris 22 and L. acidophilus 23 converted 56 and 74% lactose into lactic acid, respectively. Effect of freeze-drying was also recorded and the results showed that in all cases there was a slight decrease in the cell count before and after the freeze-drying. The decrease was approximately 0.47, 0.078 and 0.86% for S. lactis 20, S. cremoris 22 and L. acidophilus 23, respectively. Starter culture was prepared from strains isolated from camel milk. Camel and buffalo milk cheese was prepared by using starter culture. The strains isolated from camel milk were best for acid production and coagulated the milk in less time. It is concluded that cheese can be prepared successfully from camel milk and better results can be obtained by coagulating milk with starter culture.

  14. Design, Fabrication, and Operation of Innovative Microalgae Culture Experiments for the Purpose of Producing Fuels: Final Report, Phase I

    Energy Technology Data Exchange (ETDEWEB)

    1985-01-01

    A conceptual design was developed for a 1000-acre (water surface) algae culture facility for the production of fuels. The system is modeled after the shallow raceway system with mixing foils that is now being operated at the University of Hawaii. A computer economic model was created to calculate the discounted breakeven price of algae or fuels produced by the culture facility. A sensitivity analysis was done to estimate the impact of changes in important biological, engineering, and financial parameters on product price.

  15. Effect of airstream velocity on mean drop diameters of water sprays produced by pressure and air atomizing nozzles

    Science.gov (United States)

    Ingebo, R. D.

    1977-01-01

    A scanning radiometer was used to determine the effect of airstream velocity on the mean drop diameter of water sprays produced by pressure atomizing and air atomizing fuel nozzles used in previous combustion studies. Increasing airstream velocity from 23 to 53.4 meters per second reduced the Sauter mean diameter by approximately 50 percent with both types of fuel nozzles. The use of a sonic cup attached to the tip of an air assist nozzle reduced the Sauter mean diameter by approximately 40 percent. Test conditions included airstream velocities of 23 to 53.4 meters per second at 293 K and atmospheric pressure.

  16. The examination of Hevea brasiliensis plants produced by in vitro culture and mutagenesis by DNA fingerprinting techniques

    International Nuclear Information System (INIS)

    Low, F.C.; Atan, S.; Jaafar, H.

    1998-01-01

    Rubber (Hevea brasiliensis) plants derived from anther and ovule culture as well as gamma-irradiated plants were examined by several DNA marker techniques. These include restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNA (RAPD), sequence tagged microsatellite sites (STMS), DNA amplification fingerprinting (DAF) and amplified fragment length polymorphisms (AFLPs). Compared to control plants produced by vegetative propagation (cutting and budding), plants produced by in vitro culture appeared to have a reduction in the number of rDNA loci. Two RAPD protocols were compared and found to be similar in amplification of the major DNA bands. After confirmation that the RAPD method adopted was reproducible, the technique was applied to the present studies. Eight out of the 60 primers screened were able to elicit polymorphisms between pooled DNA from in vitro culture plants. Variations in DNA patterns were observed between pooled DNA samples of anther-derived plants as well as between anther-derived and ovule-derived plants. Comparisons of RAPD patterns obtained between anther-derived plants exposed to increasing dosages of gamma-irradiation with non irradiated anther-derived plants revealed distinct DNA polymorphisms. The changes in DNA profiles did not appear to be correlated to the dosage of irradiation. Since somaclonal variation was detected, it was difficult to identify changes which were specifically caused by irradiation. Application of the STMS technique to tag micro satellite sequences (GA) n , (TA) n and (TTA) n in the hydroxymethylglutaryl coenzyme A reductase-1 (hmgr-1) gene failed to detect differences between plants derived from anther and ovule culture. Although restriction endonuclease digestions with methylation sensitive enzymes suggested that four in vitro culture plants examined exhibited similar digestion patterns as the controls, a change in cytosine methylation in one anther-derived plant was detected. Examination of

  17. Isolation and Characterization of an Amylase Producing Yeast and its Application in Carotenoid Production Using Dual Culture

    Directory of Open Access Journals (Sweden)

    Iraj Nahvi

    2005-06-01

    Full Text Available Starch is a plant polysaccharide with unique applications in Iran. Its increasing production and processing recently have led to large volumes of industrial effluent as an environmental pollutant. In this study, an amylase producing yeast is isolated and identified as “Cryptococcus aerius” to investigate some of its characteristics such as its amylase secretion and starch digesting patterns, kinetics of amylase complex, and its capability for carotenoid production in dual culture. The results indicate that C.aerius is capable of soluble and raw maize starch digestion and assimilation. Raw starch digestion is scarce among yeast species; hence, it is industrially important. C.aerius digests soluble starch in the first 10 hours of cultivation and on the basis of amylase secreting patterns, it is therefore categorized with fast growing species on starch as carbon source. Non-pathogenicity, digestion of raw starch, heat stability of the secreted amylases complex (>55˚C, and the optimum pH level of 5.5- 6 for amylases complex are the set of properties that make this species capable of use in microbial production on an industrial scale. Absorption of carotenoid extract obtained from dual fermentation of C.aerius and Rhodotorula sp. indicates that the quality of carotenoids produced in dual fermentation is the same as that produced from pure Rhodotorula sp culture.

  18. Cultural based preconceptions in aesthetic experience of architecture

    Directory of Open Access Journals (Sweden)

    Stevanović Vladimir

    2011-01-01

    Full Text Available On a broader scale, the aim of this paper is to examine theoretically the effects a cultural context has on the aesthetic experience of images existing in perceived reality. Minimalism in architecture, as direct subject of research, is a field of particularities in which we observe functioning of this correlation. Through the experiment with the similarity phenomenon, the paper follows specific manifestations of general formal principles and variability of meaning of minimalism in architecture in limited areas of cultural backgrounds of Serbia and Japan. The goal of the comparative analysis of the examples presented is to indicate the conditions that may lead to a possibly different aesthetic experience in two different cultural contexts. Attribution of different meanings to similar formal visual language of architecture raises questions concerning the system of values, which produces these meanings in their cultural and historical perspectives. The establishment of values can also be affected by preconceptions resulting from association of perceived similarities. Are the preconceptions in aesthetic reception of architecture conditionally affected by pragmatic needs, symbolic archetypes, cultural metaphors based on tradition or ideologically constructed dogmas? Confronting philosophical postulates of the Western and Eastern traditions with the transculturality theory of Wolfgang Welsch, the answers may become more available.

  19. Cultural Analysis - towards cross-cultural understanding

    DEFF Research Database (Denmark)

    Gullestrup, Hans

    The book considers intercultural understanding and co-action, partly by means of general insight into concept of culture and the dimensions which bring about cultural differences, and partly as a methodology to analyse a certain culture - whether one's own or others'. This leads towards an unders......The book considers intercultural understanding and co-action, partly by means of general insight into concept of culture and the dimensions which bring about cultural differences, and partly as a methodology to analyse a certain culture - whether one's own or others'. This leads towards...... a theoretical/abstract proposal for cultural understanding. The second part presents a theoretical/abstract proposal for under-standing intercultural plurality and complexity. The third part provides an empirical model for the analysis of intercultural co-action. Finally, the fourth part present and discusses...

  20. ISOLATION, CHARACTERIZATION MORPHOLOGICAL AND EVALUATION OF MYCELIAL GROWTH AND SPORULATION OF VARIOUS MEANS CULTURE OF Quambalaria sp.

    Directory of Open Access Journals (Sweden)

    Ediellen Mayara Corrêa Gomes

    2016-12-01

    Full Text Available The characterization of microorganisms is strategic for plant protection. The objective was to analyze the morphological characteristics and the mycelial growth and sporulation on different culture media fungus Quambalaria sp. Morphological characterization was based on color, type of colonies, type and size of spores were used taxonomic keys and specialized literature for description and identification of fungal structures. For the assessment of mycelial growth and sporulation were used means potato dextrose agar culture (PDA, vegetable-agar (V8 agar and oatmeal agar (OA. two isolates were obtained denominated Q1 and Q2 showed that different types of colonies and mycelial growth, and the spores of both characterized as hyaline, miniature format with obovoid the fusiform and its chambered hyphae. A linear relationship between sporulation and mycelial growth was observed, that is, the larger the sporulation, the greater mycelial growth. The means of V8-agar culture was the most stimulated mycelial growth and sporulation isolated Q1 and the BDA through the isolated Q2, with the MIGS of 11.07 and 10.57 mm day-1, respectively. The results of this study allow us to base the start of a study on the Quambalaria gender, in northern Brazil and to add to the few reported studies on this pathosystem. Keywords: leaf spot; eucalyptus; phytopathogen; forest pathology.

  1. Nearly Finished Genomes Produced Using Gel Microdroplet Culturing (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    Energy Technology Data Exchange (ETDEWEB)

    Fitzsimmons, Michael

    2012-06-01

    Michael Fitzsimmons from Los Alamos National Laboratory gives a talk titled "Nearly Finished Genomes Produced Using Gel Microdroplet Culturing" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  2. "This Guy Is Japanese Stuck in a White Man's Body": A Discussion of Meaning Making, Identity Slippage, and Cross-Cultural Adaption.

    Science.gov (United States)

    Armour, William S.

    2001-01-01

    Addresses two issues within a general theory of cross-cultural adaption. One concerns the extent to which cross-cultural adaption is activated by the ability to make meaning in Japanese as a foreign language; the second investigated the phenomenon of identity slippage. Six life histories of informants who had learned Japanese after age 11 are used…

  3. Microstructure and Textural Properties of Yoghurts Produced by Exopolysaccharides- Producing Starter Cultures

    DEFF Research Database (Denmark)

    Zhang, Lanjun

    conferred a ropy texture and resulted in yoghurts with decreased water holding capacity and an open microstructure. In addition, one of the LB strains with high amounts of EPS producing capacity improved water retention, when it was combined with an ST strain that produced negligible amounts of EPS...

  4. Development of a chemically defined platform fed-batch culture media for monoclonal antibody-producing CHO cell lines with optimized choline content.

    Science.gov (United States)

    Kuwae, Shinobu; Miyakawa, Ichiko; Doi, Tomohiro

    2018-01-11

    A chemically defined platform basal medium and feed media were developed using a single Chinese hamster ovary (CHO) cell line that produces a monoclonal antibody (mAb). Cell line A, which showed a peak viable cell density of 5.9 × 10 6  cells/mL and a final mAb titer of 0.5 g/L in batch culture, was selected for the platform media development. Stoichiometrically balanced feed media were developed using glucose as an indicator of cell metabolism to determine the feed rates of all other nutrients. A fed-batch culture of cell line A using the platform fed-batch medium yielded a 6.4 g/L mAb titer, which was 12-fold higher than that of the batch culture. To examine the applicability of the platform basal medium and feed media, three other cell lines (A16, B, and C) that produce mAbs were cultured using the platform fed-batch medium, and they yielded mAb titers of 8.4, 3.3, and 6.2 g/L, respectively. The peak viable cell densities of the three cell lines ranged from 1.3 × 10 7 to 1.8 × 10 7  cells/mL. These results show that the nutritionally balanced fed-batch medium and feeds worked well for other cell lines. During the medium development, we found that choline limitation caused a lower cell viability, a lower mAb titer, a higher mAb aggregate content, and a higher mannose-5 content. The optimal choline chloride to glucose ratio for the CHO cell fed-batch culture was determined. Our platform basal medium and feed media will shorten the medium-development time for mAb-producing cell lines.

  5. Lipolytic Changes in Fermented Sausages Produced with Turkey Meat: Effects of Starter Culture and Heat Treatment.

    Science.gov (United States)

    Karsloğlu, Betül; Çiçek, Ümran Ensoy; Kolsarici, Nuray; Candoğan, Kezban

    2014-01-01

    In this study, the effects of two different commercial starter culture mixes and processing methodologies (traditional and heat process) on the lipolytic changes of fermented sausages manufactured with turkey meat were evaluated during processing stages and storage. Free fatty acid (FFA) value increased with fermentation and during storage over 120 d in all fermented sausage groups produced with both processing methodologies (p<0.05). After drying stage, free fatty acid values of traditional style and heat processed fermented sausages were between 10.54-13.01% and 6.56-8.49%, respectively. Thiobarbituric acid (TBA) values of traditionally processed fermented sausages were between 0.220-0.450 mg·kg(-1), and TBA values of heat processed fermented sausages were in a range of 0.405-0.795 mg·kg(-1). Oleic and linoleic acids were predominant fatty acids in all fermented sausages. It was seen that fermented sausage groups produced with starter culture had lower TBA and FFA values in comparison with the control groups, and heat application inhibited the lipase enzyme activity and had an improving effect on lipid oxidation. As a result of these effects, heat processed fermented sausages had lower FFA and higher TBA values than the traditionally processed groups.

  6. Flows of Meaning, Cultures of Movements

    DEFF Research Database (Denmark)

    Jensen, Ole B.

    2009-01-01

    Contemporary cities and places are defined by mobility and flows as much as by their sedentary and fixed properties. In the words of Shane the city may be seen as configured by ‘enclaves' (fixed and bounded sites) and ‘armatures' (infrastructure channels and transit spaces). By taking point...... of departure in a critique of such a sedentary / nomad dichotomy the paper aims to move beyond these arguing for a third position of ‘critical mobility thinking'. The theoretical underpinning of this position reaches across cultural theory, human geography and into sociology. It includes a notion...

  7. Variable radiation use efficiency in rice cultures grown at different locations

    International Nuclear Information System (INIS)

    Raghuveera Rao, P.; Sailaja, B.; Desiraju, Subrahmanyam; Nageswara Rao, D.; Voleti, S.R.; Satyanarayana Reddy, P.; Sudarsana Rao, G.V.

    2012-01-01

    Variation in radiation use efficiency (RUE) amongst 81 rice cultures was assessed at Hyderabad, Maruteru and Pattambi locations. The RUE was computed for each culture at panicle initiation stage (RUE p i) and at physiological maturity (RUE m ) for each location. RUE p i and RUE m differed significantly amongst cultures and also between locations. The interaction between location and cultures was also found to be highly significant. The mean RUE p i for all the cultures was 1.08, 0.56 and 1.22 g MJ -1 , respectively, for HYD, MTU and PTB. The average RUE m estimated was 0.37, 0.60 and 0.93, respectively, for HYD, MTU and PTB. Out of the 81 rice cultures, very few entries consistently showed high RUE at all the three locations and at both the stages. The cultures lET 21023 and lET 20986 recorded higher, though not the highest, RUE, at panicle initiation and maturity stages at all the three locations and produced TDM and grain yield higher than the mean TDM and grain yield for all the test cultures. (author)

  8. O cinema como pedagogia cultural: significações por mulheres idosas Cinema as cultural pedagogy: meanings by elderly women

    Directory of Open Access Journals (Sweden)

    Wânia Ribeiro Fernandes

    2010-04-01

    Full Text Available Este artigo, fundamentado no campo dos estudos culturais - sobretudo em noções foucaultianas - e dos estudos feministas, analisa a contribuição do cinema na formação das identidades de gênero de mulheres idosas. Após breve discussão de aspectos relacionados à cultura, ao poder e à conformação das identidades de gênero, buscamos identificar, através das produções discursivas, significados construídos por um grupo de mulheres idosas sobre filmes a que assistiram em sua juventude, evidenciando os efeitos que os discursos dessa mídia exerceram na construção e na produção identitária dessas mulheres.Using the theoretical reference of the cultural studies- mainly some foucauldian and feminist studies notions- this paper focuses upon the influence of films on the construction of gender identities. After a brief discussion on aspects of culture, power and identities formation, the meanings established by a group of elderly women on films they have attended during their youth are analyzed. We bring into evidence the effects that thediscourses present in that media had upon those women's identitary construction and production.

  9. Cultural Appropriations; Ethnic-Racial Representations; Black Press

    Directory of Open Access Journals (Sweden)

    Maria Angélica Zubaran

    2015-12-01

    Full Text Available This paper investigates the exchange and circulation of ideas in the black diaspora, particularly in the newspaper The Example, mapping and discussing the ethnic-racial and gender representations constructed in the narratives produced by the editors of this newspaper, during the campaign for the construction of a monument to the “Black Mother”. The aim is to analyze how the newspaper’s editors have appropriated texts that circulated in other newspapers about the campaign to the monument of the “Black Mother”, adapted them to their own interests and given them new meanings. From the theoretical approach of Cultural Studies, we understand the black press as a cultural artifact that not only informs but also produces discourses and representations that contribute to the formation of black subjectivities and identities.

  10. Similarities and Differences in Meaning in Six Cultures.

    Science.gov (United States)

    Herrmann, Douglas J.; Raybeck, Douglas

    1981-01-01

    Employed multidimensional scaling to investigate semantic domains. Studied two semantic categories that varied in abstractness (animals and emotions) in six cultures (Greece, Haiti, Hong Kong, Spain, United States, and Vietnam). (Author/MK)

  11. Culture-specific familiarity equally mediates action representations across cultures.

    Science.gov (United States)

    Umla-Runge, Katja; Fu, Xiaolan; Wang, Lamei; Zimmer, Hubert D

    2014-01-01

    Previous studies have shown that we need to distinguish between means and end information about actions. It is unclear how these two subtypes of action information relate to each other with theoretical accounts postulating the superiority of end over means information and others linking separate means and end routes of processing to actions of differential meaningfulness. Action meaningfulness or familiarity differs between cultures. In a cross-cultural setting, we investigated how action familiarity influences recognition memory for means and end information. Object directed actions of differential familiarity were presented to Chinese and German participants. Action familiarity modulated the representation of means and end information in both cultures in the same way, although the effects were based on different stimulus sets. Our results suggest that, in the representation of actions in memory, end information is superordinate to means information. This effect is independent of culture whereas action familiarity is not.

  12. Organizational-pedagogic technology of formation of motor functioning culture as mean of physical fitness improvement of 5 form

    Directory of Open Access Journals (Sweden)

    A.H. Deineko

    2015-08-01

    Full Text Available Purpose: to analyze influence of organizational-pedagogic technology of formation of motor functioning’s culture of five form pupils in process of physical education; to test experimentally methodic of formation of motor functioning’s culture by means of general gymnastic in physical education system of comprehensive schools’ pupils. Material: The research was conducted in comprehensive school № 67, Kharkov. 57 pupils of five forms (5-А form - 30 pupils, 5-B - 27 pupils participated in the research. Results: we worked out organizational-pedagogic technology of formation of motor functioning’s culture “Main gymnastic at school”, which positively influenced on development of physical fitness of experimental groups’ pupils. Conclusions: it was established that under influence of selected exercises of main gymnastic and introduced competitiveness elements pupils’ movements became more accurate, plastic, acquired higher amplitude, coordination.

  13. Agency, Context and Meaning

    DEFF Research Database (Denmark)

    Folkmann, Mads Nygaard

    2014-01-01

    The paper is a meta-discursive contribution to the discussion of how design can be understood as a medium of meaning formation and questioning of meaning. Further, the paper builds on plea for the role of humanities in relation to formulate relevant questions in design through conceptualizing the...... of meaning formulation and cultural contexts and, by this, contest design. In reflecting the foundational ground of design in terms of its agency, contexts and meaning constituents, design and its questioning of meaning can be critically reframed.......The paper is a meta-discursive contribution to the discussion of how design can be understood as a medium of meaning formation and questioning of meaning. Further, the paper builds on plea for the role of humanities in relation to formulate relevant questions in design through conceptualizing...... history, 2) the question of context in and of design, i.e. which contexts give meaning to design; this question calls for interpretive models of cultural analysis of the circuit of design in acknowledging phases and aspects of production, mediation and consumption, and 3) the question of the meaning...

  14. Enzymatic activities produced by mixed Saccharomyces and non-Saccharomyces cultures: relationship with wine volatile composition.

    Science.gov (United States)

    Maturano, Yolanda Paola; Assof, Mariela; Fabani, María Paula; Nally, María Cristina; Jofré, Viviana; Rodríguez Assaf, Leticia Anahí; Toro, María Eugenia; Castellanos de Figueroa, Lucía Inés; Vazquez, Fabio

    2015-11-01

    During certain wine fermentation processes, yeasts, and mainly non-Saccharomyces strains, produce and secrete enzymes such as β-glucosidases, proteases, pectinases, xylanases and amylases. The effects of enzyme activity on the aromatic quality of wines during grape juice fermentation, using different co-inoculation strategies of non-Saccharomyces and Saccharomyces cerevisiae yeasts, were assessed in the current study. Three strains with appropriate enological performance and high enzymatic activities, BSc562 (S. cerevisiae), BDv566 (Debaryomyces vanrijiae) and BCs403 (Candida sake), were assayed in pure and mixed Saccharomyces/non-Saccharomyces cultures. β-Glucosidase, pectinase, protease, xylanase and amylase activities were quantified during fermentations. The aromatic profile of pure and mixed cultures was determined at the end of each fermentation. In mixed cultures, non-Saccharomyces species were detected until day 4-5 of the fermentation process, and highest populations were observed in MSD2 (10% S. cerevisiae/90% D. vanrijiae) and MSC1 (1% S. cerevisiae/99% C. sake). According to correlation and multivariate analysis, MSD2 presented the highest concentrations of terpenes and higher alcohols which were associated with pectinase, amylase and xylanase activities. On the other hand, MSC1 high levels of β-glucosidase, proteolytic and xylanolytic activities were correlated to esters and fatty acids. Our study contributes to a better understanding of the effect of enzymatic activities by yeasts on compound transformations that occur during wine fermentation.

  15. Culture-centered engagement with delivery of health services: co-constructing meanings of health in the Tzu Chi Foundation through Buddhist philosophy.

    Science.gov (United States)

    Dillard, Sydney J; Dutta, Mohan; Sun, Wei-San

    2014-01-01

    The shift in health communication scholarship from the narrow focus on curing to the complexly intertwined spaces of health, illness, healing, and curing attends to the dynamic cultural contexts within which meanings and practices are negotiated, directing scholarship toward alternative spaces of health care delivery. This study utilized the culture-centered approach as a theoretical lens for providing a discursive space for understanding meanings of health constituted in the practices of the Tzu Chi Foundation, an organization that offers biomedical services within the larger philosophical understandings of Buddhism with 10 million members in over 50 different countries. The emerging perspective promotes non-biomedical meanings of health through selfless giving and assistance founded in Buddhist principles, simultaneously seeking purity of the mind, body, and soul holistically. Through the negotiation of the principles driving Buddhist philosophy and the principles that shape biomedical health care delivery, this study seeks to understand the interpretive frames that circulate among foundation staff and care recipients.

  16. A Rapid Culture Technique Produces Functional Dendritic-Like Cells from Human Acute Myeloid Leukemia Cell Lines

    Directory of Open Access Journals (Sweden)

    Jian Ning

    2011-01-01

    Full Text Available Most anti-cancer immunotherapeutic strategies involving dendritic cells (DC as vaccines rely upon the adoptive transfer of DC loaded with exogenous tumour-peptides. This study utilized human acute myeloid leukemia (AML cells as progenitors from which functional dendritic-like antigen presenting cells (DLC were generated, that constitutively express tumour antigens for recognition by CD8+ T cells. DLC were generated from AML cell lines KG-1 and MUTZ-3 using rapid culture techniques and appropriate cytokines. DLC were evaluated for their cell-surface phenotype, antigen uptake and ability to stimulate allogeneic responder cell proliferation, and production of IFN-γ; compared with DC derived from normal human PBMC donors. KG-1 and MUTZ-3 DLC increased expression of CD80, CD83, CD86, and HLA-DR, and MUTZ-3 DLC downregulated CD14 and expressed CD1a. Importantly, both KG-1 and MUTZ-3-derived DLC promoted proliferation of allogeneic responder cells more efficiently than unmodified cells; neither cells incorporated FITC-labeled dextran, but both stimulated IFN-γ production from responding allogeneic CD8+ T cells. Control DC produced from PBMC using the FastDC culture also expressed high levels of critical cell surface ligands and demonstrated good APC function. This paper indicates that functional DLC can be cultured from the AML cell lines KG-1 and MUTZ-3, and FastDC culture generates functional KG-1 DLC.

  17. Cultural ergonomics in interactional and experiential design: conceptual framework and case study of the Taiwanese twin cup.

    Science.gov (United States)

    Lin, Chih-Long; Chen, Si-Jing; Hsiao, Wen-Hsin; Lin, Rungtai

    2016-01-01

    Cultural ergonomics is an approach that considers interaction- and experience-based variations among cultures. Designers need to develop a better understanding of cultural ergonomics not just to participate in cultural contexts but also to develop interactive experiences for users. Cultural ergonomics extends our understanding of cultural meaning and our ability to utilize such understanding for design and evaluate everyday products. This study aims to combine cultural ergonomics and interactive design to explore human-culture interaction in user experiences. The linnak is a typical Taiwanese aboriginal cultural object. This study examined the cultural meaning and operational interface of the linnak, as well as the scenarios in which it is used in interaction and user experiences. The results produced a cultural ergonomics interface for examining the manner in which designers communicate across cultures as well as the interweaving of design and culture in the design process. Copyright © 2015 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  18. Desacralising Shakespeare’s “word” by means of cultural translation/transposition

    Directory of Open Access Journals (Sweden)

    José Roberto O’Shea

    2016-12-01

    Full Text Available This essay addresses ways in which cultural translation/transposition can ultimately bring about a positive “desacralisation” of Shakespeare’s Word. The discussion starts from the notion of Shakespeare’s Word as “sacred” and of sacred writings as highly sensitive language, and proceeds to overview the importance of the notions of denotation, connotation, and context in translation. Then, the essay offers working definitions of cultural translation or cultural transposition, and of non-literal translation. Finally, the essay highlights the author’s main aims in translating Shakespeare’s theatre and offers a few examples of cultural translation/transposition in his own rendering of Shakespeare’s drama into Brazilian Portuguese.

  19. Phototrophic hydrogen production from glucose by pure and co-cultures of Clostridium butyricum and Rhodobacter sphaeroides

    Energy Technology Data Exchange (ETDEWEB)

    Fang, Herbert H.P.; Zhu, Heguang; Zhang, Tong [Centre for Environmental Engineering Research, Department of Civil Engineering, University of Hong Kong, Pokfulam Road, Hong Kong (China)

    2006-12-15

    Phototrophic hydrogen production from glucose by pure and co-cultures of Clostridium butyricum and Rhodobacter sphaeroides was studied in batch experiments. Results showed that in all batches hydrogen was produced after a lag phase of about 10h; pure culture of R. sphaeroides produced hydrogen at rates substantially lower than C. butyricum. In co-culture systems, R. sphaeroides even with cell populations 5.9 times higher still could not compete with C. butyricum for glucose. In co-culture systems, R. sphaeroides syntrophically interacted with C. butyricum, using the acetate and butyrate produced by the latter as substrate for hydrogen production. Hydrogen production was ceased in all batches when the pH was lowered to the level of pH 6.5, resulting from the accumulation of fatty acids. It was also demonstrated in this study that fluorescence in situ hybridization (FISH) was an effective means for the quantification of the relative abundance of individual bacteria in a co-culture system. (author)

  20. The natural scorpion peptide, BmK NT1 activates voltage-gated sodium channels and produces neurotoxicity in primary cultured cerebellar granule cells.

    Science.gov (United States)

    Zou, Xiaohan; He, Yuwei; Qiao, Jinping; Zhang, Chunlei; Cao, Zhengyu

    2016-01-01

    The scorpion Buthus martensii Karsch has been used in Traditional Chinese Medicine to treat neuronal diseases such as neuropathic pain, paralysis and epilepsy for thousands of years. Studies have demonstrated that scorpion venom is the primary active component. Although scorpion venom can effectively attenuate pain in the clinic, it also produces neurotoxic response. In this study, toxicity guided purification led to identify a mammalian toxin termed BmK NT1 comprising of 65 amino acid residues and an amidated C-terminus, a mature peptide encoded by the nucleotide sequence (GenBank No. AF464898). In contract to the recombinant product of the same nucleotide sequence, BmK AGAP, which displayed analgesic and anti-tumor effect, intravenous injection (i.v.) of BmK NT1 produced acute toxicity in mice with an LD50 value of 1.36 mg/kg. In primary cultured cerebellar granule cells, BmK NT1 produced a concentration-dependent cell death with an IC50 value of 0.65 μM (0.41-1.03 μM, 95% Confidence Intervals, 95% CI) which was abolished by TTX, a voltage-gated sodium channel (VGSC) blocker. We also demonstrated that BmK NT1 produced modest sodium influx in cerebellar granule cell cultures with an EC50 value of 2.19 μM (0.76-6.40 μM, 95% CI), an effect similar to VGSC agonist, veratridine. The sodium influx response was abolished by TTX suggesting that BmK NT1-induced sodium influx is solely through activation of VGSC. Considered these data together, we demonstrated that BmK NT1 activated VGSC and produced neurotoxicity in cerebellar granule cell cultures. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Prebiotic potential of L-sorbose and xylitol in promoting the growth and metabolic activity of specific butyrate-producing bacteria in human fecal culture.

    Science.gov (United States)

    Sato, Tadashi; Kusuhara, Shiro; Yokoi, Wakae; Ito, Masahiko; Miyazaki, Kouji

    2017-01-01

    Dietary low-digestible carbohydrates (LDCs) affect gut microbial metabolism, including the production of short-chain fatty acids. The ability of various LDCs to promote butyrate production was evaluated in in vitro human fecal cultures. Fecal suspensions from five healthy males were anaerobically incubated with various LDCs. L-Sorbose and xylitol markedly promoted butyrate formation in cultures. Bacterial 16S rRNA gene-based denaturing gradient gel electrophoresis analyses of these fecal cultures revealed a marked increase in the abundance of bacteria closely related to the species Anaerostipes hadrus or A. caccae or both, during enhanced butyrate formation from L-sorbose or xylitol. By using an agar plate culture, two strains of A. hadrus that produced butyrate from each substrate were isolated from the feces of two donors. Furthermore, of 12 species of representative colonic butyrate producers, only A. hadrus and A. caccae demonstrated augmented butyrate production from L-sorbose or xylitol. These findings suggest that L-sorbose and xylitol cause prebiotic stimulation of the growth and metabolic activity of Anaerostipes spp. in the human colon. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  2. Transmission ethnic-cultural senses throught national cuisine

    Directory of Open Access Journals (Sweden)

    T. I. Burdeyna

    2014-05-01

    Full Text Available Global society requires acceleration and diversification of ways data’s transmission, erasing the boundaries between people and nations. However, there always will be a certain sign that distinguishes one ethnic group among others. Ethno­cultural specificity is kept and transmitted by various means. Cuisine as one of the most important everyday realities is also transmitting ethnic and cultural meanings. Moreover, each dish is a kind of special semiotic text content. Are there reasons that motivate each of us to choose the dishes? Do our choices genetically depend on our ancestors? Or just have a set of personal culinary preferences? The national dish is one of the cultural and historical features. Every national cuisine established concepts that have appeared in the process of communication. Borsch is such a concept and the ethnic mark for Ukrainians at the same time. Cooking and eating is a synthetic type of communication that produces transmission and perception of information kinesthetic, auditory and visual methods. Except the usual everyday dishes there are festive that are becoming solemn, not average.

  3. Desacralising Shakespeare’s “word” by means of cultural translation/transposition

    Directory of Open Access Journals (Sweden)

    José Roberto O’Shea

    2016-09-01

    Full Text Available http://dx.doi.org/10.5007/2175-7968.2016v36n3p124 This essay addresses ways in which cultural translation/transposition can ultimately bring about a positive “desacralisation” of Shakespeare’s Word. The discussion starts from the notion of Shakespeare’s Word as “sacred” and of sacred writings as highly sensitive language, and proceeds to overview the importance of the notions of denotation, connotation, and context in translation. Then, the essay offers working definitions of cultural translation or cultural transposition, and of non-literal translation. Finally, the essay highlights the author’s main aims in translating Shakespeare’s theatre and offers a few examples of cultural translation/transposition in his own rendering of Shakespeare’s drama into Brazilian Portuguese.

  4. Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002.

    Science.gov (United States)

    Therien, Jesse B; Zadvornyy, Oleg A; Posewitz, Matthew C; Bryant, Donald A; Peters, John W

    2014-01-01

    The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. Here we demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC 7002. Optimal growth conditions for co-cultivation of C. reinhardtii with wild-type and mutant strains of Synechococcus sp. 7002 were established. In co-culture, acetate produced by a glycogen synthase knockout mutant of Synechococcus sp. PCC 7002 was able to support the growth of a lipid-accumulating mutant strain of C. reinhardtii defective in starch production. Encapsulation of Synechococcus sp. PCC 7002 using an alginate matrix was successfully employed in co-cultures to limit growth and maintain the stability. The ability of immobilized strains of the cyanobacterium Synechococcus sp. PCC 7002 to produce acetate at a level adequate to support the growth of lipid-accumulating strains of C. reinhartdii offers a potentially practical, photosynthetic alternative to providing exogenous acetate into growth media.

  5. Contribution of natural milk culture to microbiota, safety and hygiene of raw milk cheese produced in alpine malga

    Directory of Open Access Journals (Sweden)

    Rosaria Lucchini

    2018-04-01

    Full Text Available Processing of alpine milk in malga farms is carried out under conditions that can favor contamination by coliforms, coagulase-positive staphylococci, or pathogens such as Listeria monocytogenes. With the aim to improve the hygienic characteristics and safety of cheese produced in four malga farms the use of lyophilized Natural Milk Culture prepared with selected strains was tested. Two cheesemaking tests were carried out in the same day always starting from the same milk: in the first case following the malga recipe that uses either Natural Whey Culture or without the addition of a starter, in the second one using a Natural Milk Culture. Cheesemaking were carried out in four malga farms located in the west area of Trentino region within the same week. For hygienic and safety evaluation, aerobic colony count, coagulase-positive staphylococci, Escherichia coli, staphylococcal toxins, Listeria monocytogenes , and Salmonella spp, pH and aw were determined in raw milk from evening and morning milking, curd in vat, curd after extraction and two months-ripened cheese. Pathogens or toxins, high values of coagulase- positive staphylococci and E. coli were not found in cheese samples. However, in the curd coagulase-positive staphylococci reached values almost of 5 Log CFU/g in the two malga without starter cultures. The use of Natural Milk Culture reduced E. coli counts. In addition, DNA was extracted from cheese samples and from Natural Milk Culture and the composition of the microbial community determined by Next Generation Sequencing method. The determination of cheese microbial communities demonstrated that the use of Natural Milk Culture exerted different effects in the different malga, in any case preserving bacterial biodiversity.

  6. Wordplay in Advertising: Form, Meaning and Function

    Directory of Open Access Journals (Sweden)

    Sara Laviosa

    2015-10-01

    Full Text Available Generally speaking, wordplay (or pun is a witticism that relies for its effect on playing with different levels of language, i.e. phonological, graphological, morphological, lexical, syntactic, and textual. Puns are frequently used in commercial advertising as a rhetorical device to promote a given product or service by creating humour, attracting the reader’s attention and adding persuasive force to the message. They also reflect the cultural preferences and traditions of a country, therefore they can be fruitfully used for pedagogic purposes to raise awareness of the specific linguistic and cultural features of the foreign language. In this paper  I examine the form, meaning and function of puns that rely on the different meanings of polysemic words, the literal and non-literal senses of idioms or on bringing two homonyms together in the same utterance to produce witty remarks. After introducing the notions of homonymy, polysemy and idiom I analyse the play on words contained in a sample of advertisements selected at random from two broadsheet newspapers, The Guardian Weekend (1997 and The Independent (1997, two quality weekly magazines, Cosmopolitan (1997 and The Telegraph Magazine (1997, as well as a promotional brochure by Alliance & Leicester plc (2003 and promotional leaflets published in 2000 respectively by Royal Mail, British Telecommunications plc and Johnson & Johnson. Finally, I propose some activities that can be carried out in the LSP classroom either individually or in groups to raise awareness of some of the linguistic and cultural features characterizing the rhetoric of marketing and promotion in business language.

  7. Teaching Students to Infer Meaning through Material Culture

    Science.gov (United States)

    Brewer, Ernest Andrew; Fritzer, Penelope

    2011-01-01

    Social studies students can learn to glean historical information from the study of material culture through active engagement as curators. Teachers can guide students through a pre-survey of helpful reading materials and then through selecting items of personal significance to them: creating labels that objectively describe the chosen items as to…

  8. Production of vesicular-arbuscular mycorrhizal fungus inoculum in aeroponic culture.

    Science.gov (United States)

    Hung, L L; Sylvia, D M

    1988-02-01

    Bahia grass (Paspalum notatum) and industrial sweet potato (Ipomoea batatas) colonized by Glomus deserticola, G. etunicatum, and G. intraradices were grown in aeroponic cultures. After 12 to 14 weeks, all roots were colonized by the inoculated vesicular-arbuscular mycorrhizal fungi. Abundant vesicles and arbuscules formed in the roots, and profuse sporulation was detected intra-and extraradically. Within each fungal species, industrial sweet potato contained significantly more roots and spores per plant than bahia grass did, although the percent root colonization was similar for both hosts. Mean percent root colonization and sporulation per centimeter of colonized root generally increased with time, although with some treatments colonization declined by week 14. Spore production ranged from 4 spores per cm of colonized root for G. etunicatum to 51 spores per cm for G. intraradices. Infectivity trials with root inocula resulted in a mean of 38, 45, and 28% of bahia grass roots colonized by G. deserticola, G. etunicatum, and G. intraradices, respectively. The germination rate of G. etunicatum spores produced in soil was significantly higher than that produced in aeroponic cultures (64% versus 46%) after a 2-week incubation at 28 degrees C. However, infectivity studies comparing G. etunicatum spores from soil and aeroponic culture indicated no biological differences between the spore sources. Aeroponically produced G. deserticola and G. etunicatum inocula retained their infectivity after cold storage (4 degrees C) in either sterile water or moist vermiculite for at least 4 and 9 months, respectively.

  9. Uranium leaching using mixed organic acids produced by Aspergillus niger

    International Nuclear Information System (INIS)

    Yong-dong Wang; Guang-yue Li; De-xin Ding; Zhi-xiang Zhou; Qin-wen Deng; Nan Hu; Yan Tan

    2013-01-01

    Both of culture temperature and pH value had impacts on the degree of uranium extraction through changing types and concentrations of mixed organic acids produced by Aspergillus niger, and significant interactions existed between them though pH value played a leading role. And with the change of pH value of mixed organic acids, the types and contents of mixed organic acids changed and impacted on the degree of uranium extraction, especially oxalic acid, citric acid and malic acid. The mean degree of uranium extraction rose to peak when the culture temperature was 25 deg C (76.14 %) and pH value of mixed organic acids was 2.3 (82.40 %) respectively. And the highest one was 83.09 %. The optimal culture temperature (25 deg C) of A. niger for uranium leaching was different from the most appropriate growing temperature (37 deg C). (author)

  10. Evolution of a recombinant (gucoamylase-producing) strain of Fusarium venenatum A3/5 in chemostat culture.

    Science.gov (United States)

    Wiebe, M G; Robson, G D; Shuster, J; Trinci, A P

    2001-04-20

    Fusarium venenatum JeRS 325 is a transformant of strain A3/5 which produces Aspergillus niger glucoamylase (GAM) under the control of a Fusarium oxysporum trypsin-like protease promoter. The evolution of JeRS 325 was studied in glucose-limited chemostat cultures grown on NaNO3 or (NH4)2SO4 as the nitrogen source. Thirteen mutants which were more highly branched and four mutants which were more sparsely branched than the parental strain were isolated from the NaNO3 chemostat. The highly branched mutants detected in this chemostat did not displace the sparsely branched population. The mutants isolated from the NaNO3 chemostat complemented representative strains previously isolated from glucose-limited chemostat cultures of F. venenatum A3/5 grown on (NH4)2SO4, but showed little complementation between themselves. By contrast, a highly branched mutant isolated from the (NH4)2SO4 chemostat culture displaced the sparsely branched mycelial population. None of the mutants isolated from the NaNO3 or (NH4)2SO4 chemostats produced as much GAM as JeRS 325. Southern blot analysis showed that all except one mutant had lost copies of both the glucoamylase and the acetamidase (the selectable marker) genes. However, specific GAM production was not necessarily correlated with the extent of glaA gene loss observed. Further, 10 of the mutants had lost the ability to grow on acetamide as the sole nitrogen source, although they retained copies of the amdS gene. In competition studies, mutants which could not utilize acetamide displaced mutants which could. The presence of foreign DNA in JeRS 325 resulted in a reduced specific growth rate (compared to A3/5), but the presence of the foreign DNA did not prevent the evolution of the strain or the isolation of mutants which had improved growth rates. Copyright 2001 John Wiley & Sons, Inc.

  11. Screening and selection of high carotenoid producing in vitro tomato cell culture lines for [13C]-carotenoid production.

    Science.gov (United States)

    Engelmann, Nancy J; Campbell, Jessica K; Rogers, Randy B; Rupassara, S Indumathie; Garlick, Peter J; Lila, Mary Ann; Erdman, John W

    2010-09-22

    Isotopically labeled tomato carotenoids, phytoene, phytofluene, and lycopene, are needed for mammalian bioavailability and metabolism research but are currently commercially unavailable. The goals of this work were to establish and screen multiple in vitro tomato cell lines for carotenoid production, test the best producers with or without the bleaching herbicides, norflurazon and 2-(4-chlorophenyl-thio)triethylamine (CPTA), and to use the greatest carotenoid accumulator for in vitro 13C-labeling. Different Solanum lycopersicum allelic variants for high lycopene and varying herbicide treatments were compared for carotenoid accumulation in callus and suspension culture, and cell suspension cultures of the hp-1 line were chosen for isotopic labeling. When grown with [U]-13C-glucose and treated with CPTA, hp-1 suspensions yielded highly enriched 13C-lycopene with 45% of lycopene in the M+40 form and 88% in the M+35 to M+40 isotopomer range. To the authors' knowledge this is the first report of highly enriched 13C-carotenoid production from in vitro plant cell culture.

  12. Unusual Δ7,12,19 C35:3 Alkenone Produced by the Mutant Emiliania huxleyi strain CCMP2758 in Culture

    Science.gov (United States)

    Zheng, Y.; Huang, Y.; Zhang, Y.; Dillon, J. T.

    2015-12-01

    Alkenones with chain length ranging from C37 to C40 are highly specific biomarkers for certain haptophyte algae in ocean and lake sediments and have been widely used for paleoclimate studies. Short chain alkenones (e.g., C35 and C36) have been found in environmental and culture samples but the origin and structures of these compounds are not fully understood. The benchmark marine alkenone producer, Emiliania huxleyi CCMP2758 strain (the mutant of strain CCMP1742, NEPCC55a) was reported to make 35:2 alkenone when cultured at 15 °C (Prahl et al., 2006). Here we show, when this strain is cultured at lower temperatures (e.g., 4°C), CCMP2758 produces large amount of 35:3 alkenone with unusual double bond positions of Δ7,12,19. We determined the double bond positions of the C35:3 methyl ketonee based on GC-MS analysis of cyclobutylimine derivatives and dimethyl disulfide derivatives respectively, and provide the first temperature calibrations based on the unsaturation ratios of C35 alkenones. Previous studies have found 35:2 alkenone with three methylene interruption in the Black Sea sediment, but it is the first time that an alkenone with a mixed three and five methylene interruption is found. The discovery of short chain alkenones with unusual double bond positions may shed new light to alkenone biosynthesis.

  13. Mean flow produced by small-amplitude vibrations of a liquid bridge with its free surface covered with an insoluble surfactant

    Science.gov (United States)

    Carrión, Luis M.; Herrada, Miguel A.; Montanero, José M.; Vega, José M.

    2017-09-01

    As is well known, confined fluid systems subject to forced vibrations produce mean flows, called in this context streaming flows. These mean flows promote an overall mass transport in the fluid that has consequences in the transport of passive scalars and surfactants, when these are present in a fluid interface. Such transport causes surfactant concentration inhomogeneities that are to be counterbalanced by Marangoni elasticity. Therefore, the interaction of streaming flows and Marangoni convection is expected to produce new flow structures that are different from those resulting when only one of these effects is present. The present paper focuses on this interaction using the liquid bridge geometry as a paradigmatic system for the analysis. Such analysis is based on an appropriate post-processing of the results obtained via direct numerical simulation of the system for moderately small viscosity, a condition consistent with typical experiments of vibrated millimetric liquid bridges. It is seen that the flow patterns show a nonmonotone behavior as the Marangoni number is increased. In addition, the strength of the mean flow at the free surface exhibits two well-defined regimes as the forcing amplitude increases. These regimes show fairly universal power-law behaviors.

  14. Isolation and molecular characterization of phytase producing ...

    African Journals Online (AJOL)

    Isolation and molecular characterization of phytase producing bacteria from Malaysia hot springs. ... The strains were further analyzed in broth culture using sodium phytate as substrate. Among them, strain L3 was selected as the best producer (0.16 U/ml after 72 h of culture). This phytase showed optimal activity at 37 °C ...

  15. The effect of cultural interaction on cumulative cultural evolution.

    Science.gov (United States)

    Nakahashi, Wataru

    2014-07-07

    Cultural transmission and cultural evolution are important for animals, especially for humans. I developed a new analytical model of cultural evolution, in which each newborn learns cultural traits from multiple individuals (exemplars) in parental generation, individually explores around learned cultural traits, judges the utility of known cultural traits, and adopts a mature cultural trait. Cultural evolutionary speed increases when individuals explore a wider range of cultural traits, accurately judge the skill level of cultural traits (strong direct bias), do not strongly conform to the population mean, increase the exploration range according to the variety of socially learned cultural traits (condition dependent exploration), and make smaller errors in social learning. Number of exemplars, population size, similarity of cultural traits between exemplars, and one-to-many transmission have little effect on cultural evolutionary speed. I also investigated how cultural interaction between two populations with different mean skill levels affects their cultural evolution. A population sometimes increases in skill level more if it encounters a less skilled population than if it does not encounter anyone. A less skilled population sometimes exceeds a more skilled population in skill level by cultural interaction between both populations. The appropriateness of this analytical method is confirmed by individual-based simulations. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. Do I mean what I say and say what I mean? A cross cultural ap-proach to the use of emoticons & emojis in CMC messages

    Directory of Open Access Journals (Sweden)

    Lifen CHENG

    2017-12-01

    Full Text Available The use of emoticons and emojis among online messaging users has achieved a globalized level. This article aims at examining how certain emojis and emoticons are chosen by message senders to regular the intensity of emotions in their messages. Or, by means of using them, message senders wish to blur textual rigidity and show interaction empathy during the communication process. This study analyzes the use of these symbols and graphic elements in message exchange through CMC and SNS, where users from different culture background convey their feeling. For it, two empirical studies of a methodological design were carried out: One pilot study was intended for the research instrument validation. The second was a survey study. By testing online messaging habit, this study enabled the researchers to observe whether the senders inclined to linguistic text use only, or they preferred including an additional emoticon or emoji in their message to communicate their feelings. Results of this research show that, at a certain extent, massage senders’ different culture background could influence on their emotional expressivity level.

  17. Technical means and system guarantee of museumprotection and inheritance for intangible cultural heritage

    Institute of Scientific and Technical Information of China (English)

    TANG Zhiqiang

    2015-01-01

    Intangible cultural heritage has a rich connotation of unique thinking way, ethical imaginationand cultural awareness, which is an important sign of national spiritual culture. Possessing the specialvalues of professionalism, permanency, security and openness, with the advantages museums play acentral role in inheriting and rescuing our intangible cultural heritage. Museums have a lot of technicalmeans for museums to protect and inherit intangible cultural heritage, such as scientific research,preservation of material carrier, museum publications, displays on intangible cultural heritage, estab-lishing thematic museums. In intangible cultural heritage, agricultural heritage is the most importantpart. The core content of which is the ideas and methods of harmony between mankind and environ-ment. There is a variety of practices on the protection by China National Agricultural Museum.

  18. Integrative studies of cultural evolution: crossing disciplinary boundaries to produce new insights.

    Science.gov (United States)

    Kolodny, Oren; Feldman, Marcus W; Creanza, Nicole

    2018-04-05

    Culture evolves according to dynamics on multiple temporal scales, from individuals' minute-by-minute behaviour to millennia of cultural accumulation that give rise to population-level differences. These dynamics act on a range of entities-including behavioural sequences, ideas and artefacts as well as individuals, populations and whole species-and involve mechanisms at multiple levels, from neurons in brains to inter-population interactions. Studying such complex phenomena requires an integration of perspectives from a diverse array of fields, as well as bridging gaps between traditionally disparate areas of study. In this article, which also serves as an introduction to the current special issue, we highlight some specific respects in which the study of cultural evolution has benefited and should continue to benefit from an integrative approach. We showcase a number of pioneering studies of cultural evolution that bring together numerous disciplines. These studies illustrate the value of perspectives from different fields for understanding cultural evolution, such as cognitive science and neuroanatomy, behavioural ecology, population dynamics, and evolutionary genetics. They also underscore the importance of understanding cultural processes when interpreting research about human genetics, neuroscience, behaviour and evolution.This article is part of the theme issue 'Bridging cultural gaps: interdisciplinary studies in human cultural evolution'. © 2018 The Author(s).

  19. How culture affects management?

    OpenAIRE

    Billi, Lorena

    2012-01-01

    The study is about how culture affects management. Culture can have many different meanings. Management has also many different ways to be approached. While doing research about cultures, the study will try to analyze how the culture affects the management. The study starts with a full explanation of the meaning of culture. Some previous analysis and studies are added to illustrate my study on the subject. The effect culture has on management is studied at different levels. The study does not...

  20. Growth of nutrient-replete Microcystis PCC 7806 cultures is inhibited by an extracellular signal produced by chlorotic cultures.

    Science.gov (United States)

    Dagnino, Denise; de Abreu Meireles, Diogo; de Aquino Almeida, João Carlos

    2006-01-01

    The frequency of cyanobacterial blooms has been increasing all over the world. These blooms are often toxic and have become a serious health problem. The aim of this work was to search for population density control mechanisms that could inhibit the proliferation of the toxic bloom-forming genus Microcystis. Microcystis PCC 7806 cultured for long periods in liquid ASM-1 medium loses its characteristic green colour. When a medium of chlorotic cultures is added to a nutrient-replete culture, cell density increase is drastically reduced when compared with controls. Inhibition of cell proliferation occurs in Microcystis cultures from any growth stage and was not strain-specific, but other genera tested showed no response. Investigations on the mechanism of growth inhibition showed that cultures treated with the conditioned medium acquired a pale colour, with pigment concentration similar to that found in chlorotic cultures. Ultrastructural examination showed that the conditioned medium induced thylakoid membrane disorganization, typical of chlorotic cells, in nutrient-replete cultures. An active extract was obtained and investigations showed that activity was retained after heating and after addition of an apolar solvent. This indicates that activity of the conditioned medium from chlorotic cells results from non-protein, apolar compound(s).

  1. Urine culture

    Science.gov (United States)

    Culture and sensitivity - urine ... when urinating. You also may have a urine culture after you have been treated for an infection. ... when bacteria or yeast are found in the culture. This likely means that you have a urinary ...

  2. On the Meaning of Cross-Cultural Differences in Simple Cognitive Measures

    Science.gov (United States)

    van de Vijver, Fons J. R.

    2008-01-01

    A set of 5 reaction time tests of increasing cognitive complexity were administered to 35 secondary school pupils in Zimbabwe and The Netherlands at 4 consecutive school days in order to explore the existence and nature of cross-cultural differences on reaction time tests measuring basic cognitive operations. No cross-cultural differences were…

  3. Interdisciplinary: Cultural competency and culturally congruent education for millennials in health professions.

    Science.gov (United States)

    Hawala-Druy, Souzan; Hill, Mary H

    2012-10-01

    The increasingly diverse multicultural and multigenerational student population in the United States requires that educators at all levels develop cultural knowledge, awareness, and sensitivity to help diverse learners fulfill their potential and to avoid cultural misunderstandings that can become obstacles or barriers to learning. The purpose of this study was to design and implement eclectic, creative, evidence-based interdisciplinary educational activities, along with culturally congruent teaching strategies, within a semester-long university course that promoted positive and culturally competent learning outcomes for culturally diverse, largely millennial students. The interdisciplinary course would prepare health professional students with the requisite knowledge and skills, through transformative learning that produces change agents, to provide culturally congruent and quality team-based care to diverse populations. This was a qualitative and quantitative study, which measured students' level of cultural awareness, competence, and proficiency pre and post the educational intervention. Instruments used for data collection included the Inventory for Assessing The Process of Cultural Competence-Student Version (IAPCC-SV) by Campinha-Bacote, course evaluations, students' feedback, and portfolio reflections. The study was conducted at a private academic institution located in the Mid-Atlantic region and the sample population included inter-professional students (N=106) from various health professions including nursing, pharmacy, and allied health sciences. Results from the pre- and post-test IAPCC-SV survey revealed that mean scores increased significantly from pre-test (60.8) to post-test (70.6). Thus, students' levels of cultural competency (awareness, knowledge, skills, desire, encounter) improved post-educational intervention, indicating that the teaching methods used in the course might be applied on a larger scale across the university system to cater to the

  4. Culturing Protozoa.

    Science.gov (United States)

    Stevenson, Paul

    1980-01-01

    Compares various nutrient media, growth conditions, and stock solutions used in culturing protozoa. A hay infusion in Chalkey's solution maintained at a stable temperature is recommended for producing the most dense and diverse cultures. (WB)

  5. Effect of different in vitro culture extracts of black pepper (Piper nigrum L.) on toxic metabolites-producing strains.

    Science.gov (United States)

    Ahmad, Nisar; Abbasi, Bilal Haider; Fazal, Hina

    2016-03-01

    In the present study, the effect of different in vitro cultures (callus, in vitro shoots) and commercially available peppercorn extract was investigated for its activity against toxic metabolite-producing strains (Escherichia coli, Pseudomonas aeroginosa, Salmonella typhi, Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, and Candida albicans). These in vitro cultures were extracted with ethanol, hexane, and chloroform, and the antipathogenic activity was determined by well-diffusion method. Hexane extract of callus showed 22 mm zone of inhibition against B. cereus, 23 mm against S. aureus, while regenerated shoots and seeds have shown 24.3 and 26 mm zones of inhibition. The ethanolic extracts of regenerated Piper shoots have shown 25 mm activity against S. aureus, 21 mm against B. cereus, and 16 mm in the case of C. albicans in comparison with standard antibiotics. Peppercorn extracts in chloroform and ethanol had shown activities against B. cereus (23.6 mm) and B. subtilis (23.5 mm). During in vitro organogenesis and morphogenesis, cells and tissues produced a comparable phytochemicals profile like mother plant. Morphogenesis is critically controlled by the application of exogenous plant-growth regulators. Such addition alters the hormonal transduction pathways, and cells under in vitro conditions regenerate tissues, which are dependant on the physiological state of cells, and finally enhance the production of secondary metabolites. To the best of our knowledge, this is the first report to compare the antimicrobial potential of in vitro regenerated tissues and peppercorn with standard antibiotics. In conclusion, most of the extracts showed pronounced activities against all the pathogenic microbes. This is a preliminary work, and the minimum inhibitory concentration values needs to be further explored. Regenerated tissues of P. nigrum are a good source of biologically active metabolites for antimicrobial activities, and callus culture presented itself as

  6. On some methods to produce high-energy polarized electron beams by means of proton synchrotrons

    International Nuclear Information System (INIS)

    Bessonov, E.G.; Vazdik, Ya.A.

    1980-01-01

    Some methods of production of high-energy polarized electron beams by means of proton synchrotrons are considered. These methods are based on transfer by protons of a part of their energy to the polarized electrons of a thin target placed inside the working volume of the synchrotron. It is suggested to use as a polarized electron target a magnetized crystalline iron in which proton channeling is realized, polarized atomic beams and the polarized plasma. It is shown that by this method one can produce polarized electron beams with energy approximately 100 GeV, energy spread +- 5 % and intensity approximately 10 7 electron/c, polarization approximately 30% and with intensity approximately 10 4 -10 5 electron/c, polarization approximately 100% [ru

  7. Capacitors for Integrated Circuits Produced by Means of a Double Implantation Method

    International Nuclear Information System (INIS)

    Zukowski, P.; Partyka, J.; Wegierek, P.

    1998-01-01

    The paper presents a description of a method to produce capacitors in integrated circuits that consists in implanting weakly doped silicon with the same impurity, then subjecting it to annealing (producing the inner plate) and implanting it again with ions of neutral elements to produce the dielectric layer. Results of the testing capacitors produced that way are also presented. Unit capacity of C u = 4.5 nF/mm 2 at tgδ = 0.01 has been obtained. The authors are of the opinion that the interesting problem of discontinuous variations of dielectric losses and capacities considered as functions of temperature, must be viewed as an open problem. (author)

  8. National character does not reflect mean personality trait levels in 49 cultures

    DEFF Research Database (Denmark)

    Terracciano, A; Abdel-Khalek, A M; Adám, N

    2005-01-01

    ratings of 3989 people from 49 cultures and compared them with the average personality scores of culture members assessed by observer ratings and self-reports. National character ratings were reliable but did not converge with assessed traits. Perceptions of national character thus appear to be unfounded......Most people hold beliefs about personality characteristics typical of members of their own and others' cultures. These perceptions of national character may be generalizations from personal experience, stereotypes with a "kernel of truth," or inaccurate stereotypes. We obtained national character...

  9. A comparison of Culture-Free Self-Esteem Scale means from different child and adolescent groups.

    Science.gov (United States)

    Holaday, M; Callahan, K; Fabre, L; Hall, C; MacDonald, N; Mundy, M A; Owens, B; Plappert, H

    1996-06-01

    The Culture-Free Self-Esteem Inventory (CFSEI-2) was administered to 7 groups of children: 84 White Catholic school students from a New Orleans suburb, 78 White rural public school students from Virginia, 62 Hispanic Migrant student from Florida, 90 Aboriginal and White students from an isolated Canadian community, 199 African American students attending an inner city school, 60 Hispanic and White international students from Venezuela, and 61 Innuit students from isolated community in Labrador. The four elder groups also wrote three words to describe themselves (the Adjective Generation Technique [AGT]). Significant differences in responding between groups were found on all CFSEI-2 scales and for AGT favorability means. Although several possible reasons for these results are discussed, we conclude that the CFSEI-2 is not culture-free. Recommendations are: change the title of the test to avoid misrepresentation, limit test usage to elementary school children, develop an adolescent version with age appropriate language, and construct local norms before using the CFSEI-2 to make decisions about a child's self-esteem. To determine relevance of scores, a team of professionals and lay persons should review items from this or any test given to children who may be different from the normative or standardization group.

  10. Plant cell tissue culture: A potential source of chemicals

    Energy Technology Data Exchange (ETDEWEB)

    Scott, C.D.; Dougall, D.K.

    1987-08-01

    Higher plants produce many industrially important products. Among these are drugs and medicinal chemicals, essential oils and flavors, vegetable oils and fats, fine and specialty chemicals, and even some commodity chemicals. Although, currently, whole-plant extraction is the primary means of harvesting these materials, the advent of plant cell tissue culture could be a much more effective method of producing many types of phytochemicals. The use of immobilized plant cells in an advanced bioreactor configuration with excretion of the product into the reactor medium may represent the most straightforward way of commercializing such techniques for lower-value chemicals. Important research and development opportunities in this area include screening for plant cultures for nonmedical, lower-value chemicals; understanding and controlling plant cell physiology and biochemistry; optimizing effective immobilization methods; developing more efficient bioreactor concepts; and perfecting product extraction and purification techniques. 62 refs., 2 figs.

  11. Cultural neuroscience and psychopathology: prospects for cultural psychiatry.

    Science.gov (United States)

    Choudhury, Suparna; Kirmayer, Laurence J

    2009-01-01

    There is a long tradition that seeks to understand the impact of culture on the causes, form, treatment, and outcome of psychiatric disorders. An early, colonialist literature attributed cultural characteristics and variations in psychopathology and behavior to deficiencies in the brains of colonized peoples. Contemporary research in social and cultural neuroscience holds the promise of moving beyond these invidious comparisons to a more sophisticated understanding of cultural variations in brain function relevant to psychiatry. To achieve this, however, we need better models of the nature of psychopathology and of culture itself. Culture is not simply a set of traits or characteristics shared by people with a common geographic, historical, or ethnic background. Current anthropology understands culture as fluid, flexible systems of discourse, institutions, and practices, which individuals actively use for self-fashioning and social positioning. Globalization introduces new cultural dynamics and demands that we rethink culture in relation to a wider domain of evolving identities, knowledge, and practice. Psychopathology is not reducible to brain dysfunction in either its causes, mechanisms, or expression. In addition to neuropsychiatric disorders, the problems that people bring to psychiatrists may result from disorders in cognition, the personal and social meanings of experience, and the dynamics of interpersonal interactions or social systems and institutions. The shifting meanings of culture and psychopathology have implications for efforts to apply cultural neuroscience to psychiatry. We consider how cultural neuroscience can refine use of culture and its role in psychopathology using the example of adolescent aggression as a symptom of conduct disorder.

  12. The Politics of Culture

    Directory of Open Access Journals (Sweden)

    John Storey

    2017-01-01

    Full Text Available This article provides an overview over the evolution of thinking about "culture" in the work of Raymond Williams. With the introduction of Antonio Gramsci's concept of hegemony culture came to be understood as consisting of not only shared, but contested meanings as well. On the basis of this redefinition by Williams, cultural studies was able to delineate culture as the production, circulation, and consumption of meanings that become embodied and embedded in social practice.

  13. Polysaccharide-producing microalgae

    Energy Technology Data Exchange (ETDEWEB)

    Braud, J.P.; Chaumont, D.; Gudin, C.; Thepenier, C.; Chassin, P.; Lemaire, C.

    1982-11-01

    The production of extracellular polysaccharides is studied with Nostoc sp (cyanophycus), Porphiridium cruentum, Rhodosorus marinus, Rhodella maculata (rhodophyci) and Chlamydomonas mexicana (chlorophycus). The polysaccharides produced are separated by centrifugation of the culture then precipitation with alcohol. Their chemical structure was studied by infrared spectrometry and acid hydrolysis. By their rheological properties and especially their insensitivity to temperatrure and pH variations the polysaccharides produced by Porphryridium cruentum and Rhodella maculata appear as suitable candidates for industrial applications.

  14. Meanings and Implications of Culture in Sustainability Education Research

    Science.gov (United States)

    Anderson, Vince; Datta, Ranjan; Dyck, Shannon; Kayira, Jean; McVittie, Janet

    2016-01-01

    As scholars working both individually and collectively, we are interested in exploring what may be achieved through taking up the complex notion of culture in sustainability education research. In this article, we present a bricolage of research, drawing on empirical and theoretical sources that collectively establish the kind of capacity we see…

  15. Production of poly-beta-hydroxybutyric acid by microorganisms accumulated from river water using a two-stage perfusion culture system.

    Science.gov (United States)

    Morimoto, T; Tashiro, F; Nagashima, H; Nishizawa, K; Nagata, F; Yokogawa, Y; Suzuki, T

    2000-01-01

    The perfusion culture system using a shaken ceramic membrane flask (SCMF) was employed to accumulate microorganisms separated from river water and to produce poly-beta-hydroxybutyric acid (PHB). Using a two-step culture method with a single SCMF, river microorganisms were cultured by separately feeding four representative carbon sources, n-propanol, lactic acid, methanol, and formic acid. After 140 h culture, the cell concentration and PHB content respectively reached 43 g/l and 35% when a propanol medium was fed. Using a two-stage perfusion culture with twin SCMFs, the seed cell mass was increased in the first SCMF and then supplied to the second flask for PHB production. As a consequence, the cellular PHB content rose to 51% in the second SCMF, while the cell concentration gradually increased to 25 g/l after 175 h perfusion culture. These results demonstrated the utility of the two-stage perfusion culture system for developing a cheap means of producing PHB coincident with wastewater treatment.

  16. Hemoglobin promotes somatic embryogenesis in peanut cultures.

    Science.gov (United States)

    Jayabalan, N; Anthony, P; Davey, M R; Power, J B; Lowe, K C

    2004-02-01

    Critical parameters influencing somatic embryogenesis include growth regulators and oxygen supply. Consequently, the present investigation has focused on optimization of a somatic embryogenic system for peanut (Arachis hypogaea L.) through media supplementation with the auxin, picloram. The latter at 30 mg L(-1) was optimal for inducing regeneration of somatic embryos from cultured explants of zygotic embryos. In contrast, somatic embryogenesis did not occur in the absence of this growth regulator. An assessment has also been made of the beneficial effect on somatic embryogenesis and plant regeneration of the commercial hemoglobin (Hb) solution, Erythrogen. Hemoglobin at 1:50 and 1:100 (v:v) stimulated increases in mean fresh weight (up to a maximum of 57% over control), mean number of explants producing somatic embryos (15%) and mean number of somatic embryos per explant (29%).

  17. Stool Culture

    Science.gov (United States)

    ... infections and may be identified with a stool culture. Some important examples include: Escherichia coli 0157:H7 and other toxin- ... the toxin-producing C. difficile will be performed. Examples of other less common causes include: ... of stool cultures that are reported as negative usually reflect the ...

  18. Culture and social class.

    Science.gov (United States)

    Miyamoto, Yuri

    2017-12-01

    A large body of research in Western cultures has demonstrated the psychological and health effects of social class. This review outlines a cultural psychological approach to social stratification by comparing psychological and health manifestations of social class across Western and East Asian cultures. These comparisons suggest that cultural meaning systems shape how people make meaning and respond to material/structural conditions associated with social class, thereby leading to culturally divergent manifestations of social class. Specifically, unlike their counterparts in Western cultures, individuals of high social class in East Asian cultures tend to show high conformity and other-orientated psychological attributes. In addition, cultures differ in how social class impacts health (i.e. on which bases, through which pathways, and to what extent). Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Between Culture and Cultural Heritage: Curriculum Historical Preconditions as Constitutive for Cultural Relations--The Swedish Case

    Science.gov (United States)

    Brantefors, Lotta

    2015-01-01

    The aim here is to describe and discuss how different cultural meanings, offered in education, can contribute to unjust cultural relations such as othering and xenophobia. By analysing the cultural and discursive content in curricula using a (neo)pragmatic curriculum theory research method, dominating ideas, values and discourses between 1948 and…

  20. Identity and cultural plurality in spanish textbooks produced in Brazil and selected by PNLD - National Programme of Textbook

    Directory of Open Access Journals (Sweden)

    Jeferson Mundim de Souza

    2016-10-01

    Full Text Available This article aims to promote, given the current reality, in which it sets up a scenario marked by breaking cultural boundaries and understand the world as a more unified universe, reflect on existing conceptions of culture in this context. The world increasingly marked by multicultural societies, the weakening of old cultural references and the multiplication of identity statements. The teaching of foreign languages is an opportunity to place the student in planetary universe of an apparent online culture, but which increasingly reinforces different identities. In this sense, the article presented here, and promote space for a discussion of these very contemporary issues, presents the analysis of a survey of two of the four textbooks in Spanish, selected to join the National Book Program for Education East, delimiting the choice of the characteristics of space and time for analysis and construction of this writing: El arte de leer Spanish and Síntesis: course española language. Not the total analysis of the books will be presented, opting to make a cut, taking as a criterion some teaching units and activities more targeted to the theme proposed, in addition to meet the rules and brevity of this article. Such research revolved around the following questions: How do the textbooks for the teaching and learning of Spanish produced in Brazil include: a the cultural diversity of the Hispanic world? ; b the claim - with respect to their community in training and socializing (s of identity (s American latina- (s? ; and c interaction - proposal from learning strategies, with the identity formation of the Brazilian student? Therefore, we adopted the methodology of qualitative analysis and content analysis technique, taking as indicators the following categories: geographical areas and cultural areas explored in the texts; the identities of anonymous characters and famous circulating in the books; and educational activities related to cultural issues

  1. Narrating horror: the horror film as cultural construct

    OpenAIRE

    Haynes, Simon

    1997-01-01

    This thesis examines horror films through an application of cultural analysis (primarily the work of Pierre Bourdieu) to selected texts in order to answer critics employing psychoanalytic perspectives to horror. It argues that psychoanalysis misses much of the heart of horror texts through its claims that textual 'meaning' lies within individuals rather than in the society in which horror texts were produced.\\ud Bourdieu's hypotheses are applied to films, along with the work of more specific ...

  2. Culture.

    Science.gov (United States)

    Smith, Timothy B; Rodríguez, Melanie Domenech; Bernal, Guillermo

    2011-02-01

    This article summarizes the definitions, means, and research of adapting psychotherapy to clients' cultural backgrounds. We begin by reviewing the prevailing definitions of cultural adaptation and providing a clinical example. We present an original meta-analysis of 65 experimental and quasi-experimental studies involving 8,620 participants. The omnibus effect size of d = .46 indicates that treatments specifically adapted for clients of color were moderately more effective with that clientele than traditional treatments. The most effective treatments tended to be those with greater numbers of cultural adaptations. Mental health services targeted to a specific cultural group were several times more effective than those provided to clients from a variety of cultural backgrounds. We recommend a series of research-supported therapeutic practices that account for clients' culture, with culture-specific treatments being more effective than generally culture-sensitive treatments. © 2010 Wiley Periodicals, Inc.

  3. Six cloned calves produced from adult fibroblast cells after long-term culture

    Science.gov (United States)

    Kubota, Chikara; Yamakuchi, Hiroshi; Todoroki, Junichi; Mizoshita, Kazunori; Tabara, Norio; Barber, Michele; Yang, Xiangzhong

    2000-01-01

    Cloning whole animals with somatic cells as parents offers the possibility of targeted genetic manipulations in vitro such as “gene knock-out” by homologous recombination. However, such manipulation requires prolonged culture of nuclear donor cells. Previous successes in cloning have been limited to the use of cells collected either fresh or after short-term culture. Therefore, demonstration of genetic totipotency of cells after prolonged culture is pivotal to combining site-specific genetic manipulations and cloning. Here we report birth of six clones of an aged (17-year-old) Japanese Black Beef bull using ear skin fibroblast cells as nuclear donor cells after up to 3 months of in vitro culture (10–15 passages). We observed higher developmental rates for embryos derived from later passages (10 and 15) as compared with those embryos from an early passage (passage 5). The four surviving clones are now 10–12 months of age and appear normal, similar to their naturally reproduced peers. These data show that fibroblasts of aged animals remain competent for cloning, and prolonged culture does not affect the cloning competence of adult somatic donor cells. PMID:10655472

  4. Culture-specific delusions. Sense and nonsense in cultural context.

    Science.gov (United States)

    Gaines, A D

    1995-06-01

    It can be said that a definition of delusions requires the invocation of cultural understandings, standards of acceptability, as well as conceptions of reality and the forces that animate it. For these reasons, the determination of delusional or normative ideation can only be effected properly within particular cultural contexts. The cross-cultural record suggests that it is difficult to separate the delusional from the cultural; a belief that is patterened and culturally specific is, by definition a cultural, not a delusional belief. One must rely upon particular, relevant local cultural understandings to ascertain when the bounds of culture have been transgressed and meaning has given way to unshareable nonsense.

  5. Effect of culture medium on polymer production and temperature on recovery of polymer produced from newly identified Rhyzopus oryzae ST29

    Directory of Open Access Journals (Sweden)

    Tipparat Hongpattarakere

    2008-04-01

    Full Text Available Thermotolerant fungal isolate ST29 was identified by observing on cell morphology and molecular technique based on internal transcribed spacer (ITS gene to be Rhizopus oryzae. Among four culture media tested, the strain exhibited the highest growth in yeast malt extract (YM medium (4.87 g/l, followed by Sabouraud dextrose broth (SDB (4.25 g/l, potato dextrose broth (PDB (4.10 g/l and palm oil mill effluent (POME (3.29 g/l, respectively, after 4 days cultivation at 45oC. However, the strain was found to produce polymer only in POME medium at 45oC, but not in the three synthetic media tested. Effect of temperature on separation of the biopolymer produced by this fungal strain was studied by incubating the culture broth in water bath with temperatures in the range of room temperature to 70oC. The biopolymer was recovered by filtration, centrifugation, and precipitation by adding 4 volumes of 95% ethanol, then freeze-drying. These temperatures therefore had no influence on the biopolymer yields (5.58-5.78 g/l or on biomass yields (2.90-3.29 g/l.

  6. Isolation of biosurfactant-producing bacteria from the Rancho La Brea Tar Pits.

    Science.gov (United States)

    Belcher, Richard W; Huynh, Kelvin V; Hoang, Timothy V; Crowley, David E

    2012-12-01

    This research was conducted to identify culturable surfactant-producing bacterial species that inhabit the 40,000-year-old natural asphalt seep at the Rancho La Brea Tar Pits in Los Angeles, CA. Using phenanthrene, monocyclic aromatic hydrocarbons, and tryptic soy broth as growth substrates, culturable bacteria from the tar pits yielded ten isolates, of which three species of gamma-proteobacteria produced biosurfactants that accumulated in spent culture medium. Partially purified biosurfactants produced by these strains lowered the surface tension of water from 70 to 35-55 mN/m and two of the biosurfactants produced 'dark halos' with the atomized oil assay, a phenomenon previously observed only with synthetic surfactants. Key findings include the isolation of culturable biosurfactant-producing bacteria that comprise a relatively small fraction of the petroleum-degrading community in the asphalt.

  7. Culture-sensitive psychotraumatology

    Directory of Open Access Journals (Sweden)

    Ulrich Schnyder

    2016-07-01

    Full Text Available Background: Although there is some evidence of the posttraumatic stress disorder (PTSD construct's cross cultural validity, trauma-related disorders may vary across cultures, and the same may be true for treatments that address such conditions. Experienced therapists tailor psychotherapy to each patient's particular situation, to the nature of the patient's psychopathology, to the stage of therapy, and so on. In addition, culture-sensitive psychotherapists try to understand how culture enhances the meaning of their patient's life history, the cultural components of their illness and help-seeking behaviors, as well as their expectations with regard to treatment. We cannot take for granted that all treatment-seeking trauma survivors speak our language or share our cultural values. Therefore, we need to increase our cultural competencies. Methods: The authors of this article are clinicians and/or researchers from across the globe, working with trauma survivors in various settings. Each author focused on one or more specific cultural aspects of working with trauma survivors and highlighted the following aspects. Results: As a result of culture-specific individual and collective meanings linked to trauma and trauma-related disorders survivors may be exposed to (self-stigma in the aftermath of trauma. Patients who are reluctant to talk about their traumatic experiences may instead be willing to write or use other ways of accessing the painful memories such as drawing. In other cultures, community and family cohesion are crucial elements of recovery. While awareness of culture-specific aspects is important, we also need to beware of premature cultural stereotyping. When disseminating empirically supported psychotherapies for PTSD across cultures, a number of additional challenges need to be taken into account: many low and middle income countries have very limited resources available and suffer from a poor health infrastructure. Conclusions: In summary

  8. Making Material and Cultural Connections: the fluid meaning of ‘Living Electrically’ in Japan and Canada 1920–1960

    Directory of Open Access Journals (Sweden)

    Heather Chappells

    2018-05-01

    Full Text Available This article explores how material and cultural ‘connections’ to households were crucial in defining historical pathways of domestic electrification in Canada and Japan. Though relatively overlooked in the history of energy, we contend that the intricacies of household connections are important in understanding convergence and divergence in electrified ways of life. Electricity’s arrival in urban and rural homes was an uneven process in part due to geographical diversity and the variable connection policies of public or private providers. Selective insights from our two countries show that spatial irregularities in grid expansion and social inequities in the meaning of electrical service further arose out of misalignments between developers’ visions and the diverse material cultures of households. This disparity manifested in the form of disconnections between non-standard wiring and equipment, as well as in the form of consumer resistance, protests and even illicit acts as people fought to get the electrical service they desired. Instead of seeing electrification as a single, universal ideal around which all countries eventually converged, we conclude that it has always been an open-ended, negotiable and culturally fluid process.

  9. ["Birth weakness": cultural meanings of maternal impressions for infant health in Northeast Brazil].

    Science.gov (United States)

    Calvasina, Paola Gondim; Nations, Marilyn K; Jorge, Maria Salete Bessa; Sampaio, Helena Alves de Carvalho

    2007-02-01

    Pregnancy encompasses socio-cultural, historical, and affective dimensions that process various meanings in women's bodies. Each society constructs popular concepts, practices, and explanatory models that differ from the biomedical model and aim to protect the mother and fetus and foster a healthy pregnancy. This qualitative study, based on interpretative anthropology, unveils the experiences of 27 poor women and their repercussions on the malnutrition of their infants, treated at a Childhood Malnutrition Treatment Center in Fortaleza, Northeast Brazil. From January to June 2004, ethnographic and narrative interviews were conducted on so-called "birth weakness", in addition to participant observation of outpatient, nursing, and home childcare. The mothers believed that their own physical and emotional suffering and precarious nutritional status were "imprinted" on the fetus, resulting in the child's malnutrition. While the ethno-etiology of "weakness" points to factors outside the body that involuntarily affect the pregnant mother, the medical view tended to incriminate the mother herself. It is necessary to understand the mothers' narratives, sympathize with their suffering, and spawn a closer approach between the popular and biomedical concepts.

  10. A cultural take on the links between religiosity, identity, and meaning in life in religious emerging adults.

    Science.gov (United States)

    Negru-Subtirica, Oana; Tiganasu, Alexandra; Dezutter, Jessie; Luyckx, Koen

    2017-03-01

    Identity and meaning in life are core developmental assets in emerging adulthood. We analysed how religiosity is related to these intentional strivings in emerging adults enrolled in theological education, by depicting (1) identity strivings and meaning in life accounts in faith narratives (Study 1) and (2) links between personal identity and meaning in life profiles and religious beliefs, behaviours, and subjective experiences (Study 2). Both studies highlighted that a Foreclosed status, with high personal commitment and reduced exploration, was dominant in faith narratives and personal identity profiles. Also, in narratives meaning in life was reflected by a strong focus on presence of meaning through religious insights. Nonetheless, global meaning in life profiles indicated that many emerging adults were searching for a meaning in their lives, while reporting lower levels of presence of meaning. Identity Achievement and High Presence-High Search profiles were linked to the highest levels of subjective, behavioural, and cognitive religiosity. We highlighted the multidimensionality of identity and meaning in life strivings in emerging adults attending theological schools. We pointed out that even in a somewhat foreclosed cultural context (e.g., Romanian Christian Orthodox theological schools), religion represents a dynamic social and ideological context for self-development. Statement of contribution What is already known on this subject? Religious beliefs increase in emerging adults, doubled by decreases in religious behaviours, linked to an adherence to a more personal approach to religion. Religious youth are more committed to their faith and also explore identity and life meaning in relation to their religious strivings. Youth religious exemplars report close links between their religious faith and strivings for meaningful life goals. What does this study add? We investigated Christian Orthodox theology students, for whom religion is a normative dimension of

  11. A plant culture system for producing food and recycling materials with sweetpotato in space

    Science.gov (United States)

    Kitaya, Yoshiaki; Yano, Sachiko; Hirai, Hiroaki

    2016-07-01

    The long term human life support in space is greatly dependent on the amounts of food, atmospheric O2 and clean water produced by plants. Therefore, the bio-regenerative life support system such as space farming with scheduling of crop production, obtaining high yields with a rapid turnover rate, converting atmospheric CO2 to O2 and purifying water should be established with employing suitable plant species and varieties and precisely controlling environmental variables around plants grown at a high density in a limited space. We are developing a sweetpotato culture system for producing tuberous roots as a high-calorie food and fresh edible leaves and stems as a nutritive functional vegetable food in space. In this study, we investigated the ability of food production, CO2 to O2 conversion through photosynthesis, and clean water production through transpiration in the sweetpotato production system. The biomass of edible parts in the whole plant was almost 100%. The proportion of the top (leaves and stems) and tuberous roots was strongly affected by environmental variables even when the total biomass production was mostly the same. The production of biomass and clean water was controllable especially by light, atmospheric CO2 and moisture and gas regimes in the root zone. It was confirmed that sweetpotato can be utilized for the vegetable crop as well as the root crop allowing a little waste and is a promising functional crop for supporting long-duration human activity in space.

  12. TV Dizileri Yoluyla Yeniden Üretilen Tüketim Kültürü / The Re-produced Consumption Culture by TV Series

    Directory of Open Access Journals (Sweden)

    Nesrin Kula

    2012-12-01

    Full Text Available “Tüketme” terimi “tahrip etmek, harcamak, israf etmek, bitirmek” anlamlarını taşımaktadır. Jean Baudrillard ise, tüketimi kodlar ve kurallarla düzenlenmiş bir göstergeler sistemi olarak tanımlamaktadır. Tüketim tarzları kişilerin kimliklerinin birer unsuru haline gelmektedir. Özneler kimliklerini ifade etmek amacıyla metaları kullanmakta ve kişilerin toplum içindeki statüsü tükettikleri ile ölçülür hale gelmektedir. TV dizileri de, bireylere tüketilecek sonsuz nesneler yoluyla oluşturulacak yaşam tarzları ile ilgili bilgiler vermektedir. Çalışmada, seçilen on dizide üretilen anlam dünyası, yeniden üretilen tüketim ideolojisi ve oluşturulan kadın ve erkek rol modelleri çözümlenmektir. The Re-produced Consumption Culture by TV Series The concept of consumption means ‘to destroy, spend, waste or finish something’. Jean Baudrillard describes the consumption as a semiotic system regulated of codes and rules. Consumption styles are nowadays becoming an element of people’s identity. Individuals use the commodities to express their identities and therefore the status of people in the community being measured according to what they consume. Meanwhile many TV series provides information to individuals about lifestyles to be created by infinite items consumed. This study analyzes the mindset created in ten selected TV series and the re-produced ideology of consumption as well as the presented role models for women and men in them.

  13. Cybersemiotics: A suggestion for a transdisciplinary framework for description of observing, anticipatory and meaning producing systems

    Science.gov (United States)

    Brier, Soren

    1998-07-01

    The ability of systems to be anticipatory seems to be intricate connected with the ability to observe and to cognate by reducing complexity through signification. The semantic capacity of living systems, the cognitive ability to assign meaning to differences perturbating the system's self-organization, seems to be the prerequisite for the phenomenon of communication, language and consciousness. In cybernetics Bateson developed the idea that information is a difference that makes a difference and second order cybernetics developed the concept of organisms as self-organized and self-produced systems (autopoietic) as the prerequisite of life and cognition. The cognitive ability seems to be qualitative different from what so far is computable on any known machine although parts of different aspects of the process can be partly simulated in AI, neutral network and AL. In semiotics the fundamental process of cognition and communication is called semiosis or signification and C. S. Peirce created a special triadic, objective idealistic, pragmatic and evolutionary philosophy to be able to give a fruitful description of the process and its relation to logic and the concept of natural law. Both second order cybernetics and semiotics sees information and meaning as something produced by individual organisms through structural couplings to the environments or other individuals through historical drift and further developed in social communication. Luhmann points out that social communication also only functions through structural couplings which he calls generalized media such as science, art, power, love and money. Peirce talks of the semiotic net as a triadic view of meanings developing through history and in animals through evolution. In accordance with this Wittgenstein points out that signification is created in language games developed in specific life forms. Life forms are the things we do in society such as seducing, commanding and explaining. As animals do not have

  14. Of Models and Meanings: Cultural Resilience in Social–Ecological Systems

    NARCIS (Netherlands)

    Crane, T.A.

    2010-01-01

    Modeling has emerged as a key technology in analysis of social–ecological systems. However, the tendency for modeling to focus on the mechanistic materiality of biophysical systems obscures the diversity of performative social behaviors and normative cultural positions of actors within the modeled

  15. Organizational culture associated with provider satisfaction.

    Science.gov (United States)

    Scammon, Debra L; Tabler, Jennifer; Brunisholz, Kimberly; Gren, Lisa H; Kim, Jaewhan; Tomoaia-Cotisel, Andrada; Day, Julie; Farrell, Timothy W; Waitzman, Norman J; Magill, Michael K

    2014-01-01

    Organizational culture is key to the successful implementation of major improvement strategies. Transformation to a patient-centered medical home (PCHM) is such an improvement strategy, requiring a shift from provider-centric care to team-based care. Because this shift may impact provider satisfaction, it is important to understand the relationship between provider satisfaction and organizational culture, specifically in the context of practices that have transformed to a PCMH model. This was a cross-sectional study of surveys conducted in 2011 among providers and staff in 10 primary care clinics implementing their version of a PCMH: Care by Design. Measures included the Organizational Culture Assessment Instrument and the American Medical Group Association provider satisfaction survey. Providers were most satisfied with quality of care (mean, 4.14; scale of 1-5) and interactions with patients (mean, 4.12) and were least satisfied with time spent working (mean, 3.47), paperwork (mean, 3.45), and compensation (mean, 3.35). Culture profiles differed across clinics, with family/clan and hierarchical cultures the most common. Significant correlations (P ≤ .05) between provider satisfaction and clinic culture archetypes included family/clan culture negatively correlated with administrative work; entrepreneurial culture positively correlated with the Time Spent Working dimension; market/rational culture positively correlated with how practices were facing economic and strategic challenges; and hierarchical culture negatively correlated with the Relationships with Staff and Resource dimensions. Provider satisfaction is an important metric for assessing experiences with features of a PCMH model. Identification of clinic-specific culture archetypes and archetype associations with provider satisfaction can help inform practice redesign. Attention to effective methods for changing organizational culture is recommended.

  16. Silence, nostalgia, violence, poverty … : what does 'culture' mean for South African sexuality educators?

    Science.gov (United States)

    Depalma, Renée; Francis, Dennis

    2014-01-01

    In in-depth interviews with 25 Life Orientation teachers in South Africa, we found that teachers spontaneously drew upon notions of culture to explain and justify people's sexual beliefs and behaviours and their own role as educators. Drawing upon a Bakhtinian understanding of discourse, we apply critical semantic analysis to explore how culture is deployed as a discursive strategy. Teachers draw upon particular understandings of culture available to them in their social contexts. Furthermore, the substitution of the word 'culture' for a series of other phenomena (silence, violence and poverty) affords these phenomena a certain authority that they would otherwise not wield. We argue, first, that systems teacher education and training needs to (re)define culture as dynamic, interactive and responding to, but not determined by, socio-historical realities. Beyond this, teachers need to learn how to critically engage with cultural practices and perceptions and to be provided with some basic tools to do so, including more sophisticated understandings of cultural and training in dialogic methodologies. Teaching sexuality education in multicultural societies such as South Africa will require meaningful engagement in intercultural dialogues that may need to include voices that have traditionally been excluded from school spaces.

  17. Moments of joy and delight: the meaning of traditional food in dementia care.

    Science.gov (United States)

    Hanssen, Ingrid; Kuven, Britt Moene

    2016-03-01

    To learn about the meaning of traditional food to institutionalised patients with dementia. Traditional food strengthens the feelings of belonging, identity and heritage, which help persons with dementia to hold on to and reinforce their cultural identity and quality of life. Taste is more cultural than physiological. Dietary habits are established early in life and may be difficult to change. Being served unfamiliar dishes may lead to disappointment and a feeling of being betrayed and unloved. The three studies presented have a qualitative design. In-depth interviews of family members and nurses experienced in dementia care were conducted in South Africa and among ethnic Norwegians and the Sami in Norway. Content-focused analysis, hermeneutic in character, was used to enable the exploration of the thoughts, feelings and cultural meaning described. Traditional foods created a feeling of belonging and joy. Familiar tastes and smells awoke pleasant memories in patients and boosted their sense of well-being, identity and belonging, even producing words in those who usually did not speak. In persons with dementia, dishes remembered from their childhood may help maintain and strengthen cultural identity, create joy and increase patients' feeling of belonging, being respected and cared for. Traditional food furthermore improves patients' appetite, nutritional intake and quality of life. To serve traditional meals in nursing homes demands extra planning and resources, traditional knowledge, creativity and knowledge of patients' personal tastes. This study provides insight into culture-sensitive dietary needs of institutionalised patients with dementia. The cultural significance of food for feeling contentment and social and physical well-being is discussed. Besides helping to avoid undernutrition, being served traditional dishes may be very important to reminiscence, joy, thriving and quality of life. © 2016 John Wiley & Sons Ltd.

  18. Culture, Cross-Role Consistency, and Adjustment: Testing Trait and Cultural Psychology Perspectives

    OpenAIRE

    Church, A. Timothy; Anderson-Harumi, Cheryl A.; del Prado, Alicia M.; Curtis, Guy J.; Tanaka-Matsumi, Junko; Valdez Medina, José L.; Mastor, Khairul A.; White, Fiona A.; Miramontes, Lilia A.; Katigbak, Marcia S.

    2008-01-01

    Trait and cultural psychology perspectives on cross-role consistency and its relation to adjustment were examined in two individualistic cultures, the United States (N = 231) and Australia (N = 195), and four collectivistic cultures, Mexico (N = 199), Philippines (N = 195), Malaysia (N = 217), and Japan (N = 180). Cross-role consistency in trait ratings was evident in all cultures, supporting trait perspectives. Cultural comparisons of mean consistency provided support for cultural psychology...

  19. Insect cell transformation vectors that support high level expression and promoter assessment in insect cell culture

    Science.gov (United States)

    A somatic transformation vector, pDP9, was constructed that provides a simplified means of producing permanently transformed cultured insect cells that support high levels of protein expression of foreign genes. The pDP9 plasmid vector incorporates DNA sequences from the Junonia coenia densovirus th...

  20. Aging in culture.

    Science.gov (United States)

    Fung, Helene H

    2013-06-01

    This article reviews the empirical studies that test socioemotional aging across cultures. The review focuses on comparisons between Western (mostly North Americans and Germans) and Eastern cultures (mostly Chinese) in areas including age-related personality, social relationships, and cognition. Based on the review, I argue that aging is a meaning-making process. Individuals from each cultural context internalize cultural values with age. These internalized cultural values become goals that guide adult development. When individuals from different cultures each pursue their own goals with age, cultural differences in socioemotional aging occur.

  1. Strategies for efficiently selecting PHA producing mixed microbial cultures using complex feedstocks: Feast and famine regime and uncoupled carbon and nitrogen availabilities.

    Science.gov (United States)

    Oliveira, Catarina S S; Silva, Carlos E; Carvalho, Gilda; Reis, Maria A

    2017-07-25

    Production of polyhydroxyalkanoates (PHAs) by open mixed microbial cultures (MMCs) has been attracting increasing interest as an alternative technology to PHA production by pure cultures, due to the potential for lower costs associated with the use of open systems (eliminating the requirement for sterile conditions) and the utilisation of cheap feedstock (industrial and agricultural wastes). Such technology relies on the efficient selection of an MMC enriched in PHA-accumulating organisms. Fermented cheese whey, a protein-rich complex feedstock, has been used previously to produce PHA using the feast and famine regime for selection of PHA accumulating cultures. While this selection strategy was found efficient when operated at relatively low organic loading rate (OLR, 2g-CODL -1 d -1 ), great instability and low selection efficiency of PHA accumulating organisms were observed when higher OLR (ca. 6g-CODL -1 d -1 ) was applied. High organic loading is desirable as a means to enhance PHA productivity. In the present study, a new selection strategy was tested with the aim of improving selection for high OLR. It was based on uncoupling carbon and nitrogen supply and was implemented and compared with the conventional feast and famine strategy. For this, two selection reactors were fed with fermented cheese whey applying an OLR of ca. 8.5g-CODL -1 (with 3.8g-CODL -1 resulting from organic acids and ethanol), and operated in parallel under similar conditions, except for the timing of nitrogen supplementation. Whereas in the conventional strategy nitrogen and carbon substrates were added simultaneously at the beginning of the cycle, in the uncoupled substrates strategy, nitrogen addition was delayed to the end of the feast phase (i.e. after exogenous carbon was exhausted). The two different strategies selected different PHA-storing microbial communities, dominated by Corynebacterium and a Xantomonadaceae, respectively with the conventional and the new approaches. The new

  2. Cultural Meaning of Perceived Control: A Meta-Analysis of Locus of Control and Psychological Symptoms across 18 Cultural Regions

    Science.gov (United States)

    Cheng, Cecilia; Cheung, Shu-fai; Chio, Jasmine Hin-man; Chan, Man-pui Sally

    2013-01-01

    Integrating more than 40 years of studies on locus of control (LOC), this meta-analysis investigated whether (a) the magnitude of the relationship between LOC and psychological symptoms differed among cultures with distinct individualist orientations and (b) depression and anxiety symptoms yielded different patterns of cultural findings with LOC.…

  3. Meaning Structures in a Contested Issue Field

    DEFF Research Database (Denmark)

    Meyer, Renate; Höllerer, Markus

    2010-01-01

    When organizational concepts spread beyond national and cultural boundaries, they must pass through powerful filters of local cultural and structural opportunities and constraints in order to mobilize legitimacy. Struggles over their meaning are intensified if they challenge the prevailing order...

  4. Efficiency of anther culture technique in the production of wheat double haploids

    Directory of Open Access Journals (Sweden)

    Kondić-Špika Ankica Đ.

    2008-01-01

    Full Text Available The objective of the study was to investigate efficiency of anther culture in the production of spontaneous double haploids from randomly selected heterozygous genotypes of wheat (Triticum aestivum L.. Anthers of 20 F1 wheat combinations were grown in vitro on a modified Potato-2 medium. All of the examined genotypes have shown the ability to produce pollen calluses as well as to regenerate green plants. On average for the whole experiment material, 47.2 calluses were produced per 100 cultured anthers. The green plant regeneration ranged from 0.8 to 13.4 green plants per spike, with an overall mean of 5.8. From the total of 582 regenerated green plants, 47.9% (279 were spontaneous double haploids. The final average yield from the study was 2.8 double haploids per spike.

  5. OVERCOMING THE CULTURAL DIFFERENCES: PARABLE AS A MEANS OF INTERCULTURAL DIALOGUE

    Directory of Open Access Journals (Sweden)

    Tetyana V. Danylova

    2013-09-01

    Full Text Available Purpose. This article attempts to investigate the ways to overcome negative after-effects of intercultural communication. Methodology and theoretical results: To avoid the negative after-effects of intercultural communication caused by the clash of different reality tunnels, it is necessary to go beyond the framework of the specific symbolical territory, which is seen as the only true reality. Expanding the horizons of life, going beyond the boundaries of a personal reality tunnel can be achieved, in particular, by using parables. Acquaintance with parables helps to extend the set of the world perception models and to change the positions of the communication process participants. Parables act as intermediaries between people of different cultures and mentalities. Owing to its archetypical form, parable becomes one of the most popular narratives illustrating deep and multi-faceted truths that sometimes cannot be expressed by any other way. Parable serves as an open and flexible model of the world and a model of an individual "I self". This model helps people to constitute themselves as a part of their world. Parable requires to reject its interpretation in the oppositions inherent in a given culture and to reconsider the very way of understanding. Rejection of the oppositions provides the possibility of perceiving the world as integrity, unity in diversity, helps to overcome one-sided picture of the inner world of a man and his life in society. Understanding of a parable works towards changing personal position, and human creative nature is actualized. Conclusion: Parables uniquely reflect the real world and contribute to personal understanding of reality. Parables are successfully transplanted into different cultures operating as heritage of a culture-recipient and as a connecting link, which optimizes an intercultural dialogue.

  6. Culture-sensitive psychotraumatology

    Science.gov (United States)

    Schnyder, Ulrich; Bryant, Richard A.; Ehlers, Anke; Foa, Edna B.; Hasan, Aram; Mwiti, Gladys; Kristensen, Christian H.; Neuner, Frank; Oe, Misari; Yule, William

    2016-01-01

    Background Although there is some evidence of the posttraumatic stress disorder (PTSD) construct's cross cultural validity, trauma-related disorders may vary across cultures, and the same may be true for treatments that address such conditions. Experienced therapists tailor psychotherapy to each patient's particular situation, to the nature of the patient's psychopathology, to the stage of therapy, and so on. In addition, culture-sensitive psychotherapists try to understand how culture enhances the meaning of their patient's life history, the cultural components of their illness and help-seeking behaviors, as well as their expectations with regard to treatment. We cannot take for granted that all treatment-seeking trauma survivors speak our language or share our cultural values. Therefore, we need to increase our cultural competencies. Methods The authors of this article are clinicians and/or researchers from across the globe, working with trauma survivors in various settings. Each author focused on one or more specific cultural aspects of working with trauma survivors and highlighted the following aspects. Results As a result of culture-specific individual and collective meanings linked to trauma and trauma-related disorders survivors may be exposed to (self-)stigma in the aftermath of trauma. Patients who are reluctant to talk about their traumatic experiences may instead be willing to write or use other ways of accessing the painful memories such as drawing. In other cultures, community and family cohesion are crucial elements of recovery. While awareness of culture-specific aspects is important, we also need to beware of premature cultural stereotyping. When disseminating empirically supported psychotherapies for PTSD across cultures, a number of additional challenges need to be taken into account: many low and middle income countries have very limited resources available and suffer from a poor health infrastructure. Conclusions In summary, culture

  7. Cross-Cultural Counseling and Cross-Cultural Meanings: An Exploration of Morita Psychotherapy.

    Science.gov (United States)

    Aldous, Jane L.

    1994-01-01

    Describes theoretical framework and techniques of Morita psychotherapy. Western research indicates that Asian American clients prefer active-directive, logical, rational, and structured approaches. Suggests that ethnocentric counseling approaches may be imposed upon clients of Asian origin because meanings attached to terms describing counseling…

  8. BRIDGING THE COMPETING VIEWS OF EUROPEAN CULTURAL INTEGRATION: THE TRANSFORMATIVE VIEW OF CULTURE AS A MEANS TO PROMOTE GROWTH, EMPLOYMENT AND SOCIAL COHESION

    Directory of Open Access Journals (Sweden)

    María Luz SUÁREZ CASTIÑEIRA

    2018-05-01

    Full Text Available The concept of a European culture became very complex with the enlargement of 2004 towards the East, when the EU, as Delanty pointed out, moved “beyond postnationality to an encounter with multiple civilizational forms,” multiple histories and competing visions of European integration. The “unity-in-diversity” paradigm turned into a huge challenge for the European institutions. On the one hand, achieving a European image of cultural unity without excluding all the local, regional and national cultures is a very complex, if not impossible, task. On the othe hand, culture remains an ambiguous term in European institutions due to the lack of a full-fledged European cultural policy. This paper focuses first on how in the early 1970s the EC/EU started to be concerned with defining the role of culture, and second on how since the year 2000 culture has progressively acquired a new status with potentially transformative powers to bridge the competing views of cultural integration. Programmes, such as the “2014-2020 Creative Europe” programme, focus on culture as a creative accelerator and promotor of different forms of cultural participation and production. Culture generates “smart, sustainable and inclusive growth”, and contributes to “high employment, high productivity, and high social cohesion.”

  9. Lagooning microbial fuel cells: A first approach by coupling electricity-producing microorganisms and algae

    International Nuclear Information System (INIS)

    Lobato, Justo; González del Campo, Araceli; Fernández, Francisco J.; Cañizares, Pablo; Rodrigo, Manuel A.

    2013-01-01

    Highlights: • An algae cathode of a MFC has been used without artificial mediators or catalysts. • To perform a lagooning wastewater treatment coupled with energy-producing MFC. • The producing electricity operates under day/night irradiation cycles, is shown. - Abstract: The paper focused on the start-up and performance characterisation of a new type of microbial fuel cell (MFC), in which an algae culture was seeded in the cathodic chamber to produce the oxygen required to complete the electrochemical reactions of the MFC, thus circumventing the need for a mechanical aerator. The system did not use mediators or high cost catalysts and it can be started-up easily using a straightforward three-stage procedure. The start-up consists of the separate production of the electricity-producing microorganisms and the algae cultures (stage I), replacement of the mechanical aeration system by the algae culture (stage II) and a change in the light dosage from a continuous input to a dynamic day/night profile. The MFC was operated under a regime of 12 h light and 12 h dark and was also operated in batch and continuous substrate-feeding modes. The same cell voltage was achieved when the cathode compartment was operated with air supplied by aerators, which means that this configuration can perform as well as the traditional one. The results also show the influence of both the organic load and light irradiation on electricity production and demonstrate that this type MFC is a robust and promising technology that can be considered as a first approach to perform a lagooning wastewater treatment with microbial fuel cells

  10. Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata.

    Science.gov (United States)

    Kakimoto, Masayuki; Ishikawa, Toshiki; Miyagi, Atsuko; Saito, Kazuaki; Miyazaki, Motonobu; Asaeda, Takashi; Yamaguchi, Masatoshi; Uchimiya, Hirofumi; Kawai-Yamada, Maki

    2014-02-15

    A volatile metabolite, 2-methylisoborneol (2-MIB), causes an unpleasant taste and odor in tap water. Some filamentous cyanobacteria produce 2-MIB via a two-step biosynthetic pathway: methylation of geranyl diphosphate (GPP) by methyl transferase (GPPMT), followed by the cyclization of methyl-GPP by monoterpene cyclase (MIBS). We isolated the genes encoding GPPMT and MIBS from Pseudanabaena galeata, a filamentous cyanobacterium known to be a major causal organism of 2-MIB production in Japanese lakes. The predicted amino acid sequence showed high similarity with that of Pseudanabaena limnetica (96% identity in GPPMT and 97% identity in MIBS). P. galeata was cultured at different temperatures to examine the effect of growth conditions on the production of 2-MIB and major metabolites. Gas chromatograph-mass spectrometry (GC-MS) measurements showed higher accumulation of 2-MIB at 30 °C than at 4 °C or 20 °C after 24 h of culture. Real-time-RT PCR analysis showed that the expression levels of the genes encoding GPPMT and MIBS decreased at 4 °C and increased at 30 °C, compared with at 20 °C. Furthermore, metabolite analysis showed dramatic changes in primary metabolite concentrations in cyanobacteria grown at different temperatures. The data indicate that changes in carbon flow in the TCA cycle affect 2-MIB biosynthesis at higher temperatures. Copyright © 2013 Elsevier GmbH. All rights reserved.

  11. Meanings of thinness and dysfunctional eating in black South ...

    African Journals Online (AJOL)

    meanings of thinness and food refusal may not only vary from culture to culture, but ... the rapidly westernizing socio-cultural context of post-apartheid South Africa. Method: Four .... she first arrived in South Africa as an immigrant from a French-.

  12. Hospital culture--why create one?

    Science.gov (United States)

    Sovie, M D

    1993-01-01

    Hospitals, to survive, must be transformed into responsive, participative organizations capable of new practices that produce improved results in both quality of care and service at reduced costs. Creating, managing, and changing the culture are critical leadership functions that will enable the hospital to succeed. Strategic planning and effective implementation of planned change will produce the desired culture. Work restructuring, a focus on quality management along with changes in clinical practices, as well as the care and support processes, are all a part of the necessary hospital cultural revolution.

  13. Dignity and autonomy in the care for patients with dementia: Differences among formal caretakers of varied cultural backgrounds and their meaning.

    Science.gov (United States)

    Bentwich, Miriam Ethel; Dickman, Nomy; Oberman, Amitai

    A key message in the World Health Organization report on dementia (2012) emphasizes this disease as a top priority in public health and the need to improve professional attitudes to patients with dementia, while acknowledging that the workforce in dementia care is becoming increasingly diverse culturally. To trace whether there are substantial gaps between formal caretakers from different cultural groups (Israeli born Jews [Sabras], Israeli Arabs [Arabs] and migrants from Russia [Russians]) regarding their stances on the human dignity and autonomy of patients with dementia, as well as understand the meaning of these gaps. quantitative analysis utilizing questionnaires that were filled-out by approximately 200 caretakers from the different cultural groups, working in a nursing home or a hospital. In nursing homes, substantial differences were found in the attitudes to human dignity and autonomy of patients with dementia between Russian and Arab as well as Sabra caretakers. In the hospital, there was no influence for the ethno-culture variable on dignity or autonomy. Contrary to past research, in nursing homes, significant differences were found between certain ethno-cultural groups (Arabs and Russians) regarding their stance towards the dignity of patients with dementia. Arab caretakers hold a conception of dignity and autonomy that resonates strongly with person-centered care and outweighs institutional settings as well as may be related to the fostering of virtues. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  14. Decolorization of the azo dye Acid Orange 51 by laccase produced in solid culture of a newly isolated Trametes trogii strain

    OpenAIRE

    Daâssi, Dalel; Zouari-Mechichi, Héla; Frikha, Fakher; Martínez, María Jesús; Nasri, M.; Mechichi, Tahar

    2013-01-01

    This study concerns the decolorization and detoxification of the azo dye Acid Orange 51 (AO51) by crude laccase from Trametes trogii produced in solid culture using sawdust as support media. A three-level Box?Behnken factorial design with four factors (enzyme concentration, 1-hydroxybenzotriazole (HBT) concentration, dye concentration and reaction time) combined with response surface methodology was applied to optimize AO51 decolorization. A mathematical model was developed showing the effect...

  15. Beneficial effect of two culture systems with small groups of embryos on the development and quality of in vitro-produced bovine embryos.

    Science.gov (United States)

    Cebrian-Serrano, A; Salvador, I; Silvestre, M A

    2014-02-01

    Currently, in vitro-produced embryos derived by ovum pick up (OPU) and in vitro fertilization (IVF) technologies represent approximately one-third of the embryos worldwide in cattle. Nevertheless, the culture of small groups of embryos from an individual egg donor is an issue that OPU-IVF laboratories have to face. In this work, we tested whether the development and quality of the preimplantation embryos in vitro cultured in low numbers (five embryos) could be improved by the addition of epidermal growth factor, insulin, transferrin and selenium (EGF-ITS) or by the WOW system. With this aim, immature oocytes recovered from slaughtered heifers were in vitro matured and in vitro fertilized. Presumptive zygotes were then randomly cultured in four culture conditions: one large group (LG) (50 embryos/500 μl medium) and three smaller groups [five embryos/50 μl medium without (control) or with EGF-ITS (EGF-ITS) and five embryos per microwell in the WOW system (WOW)]. Embryos cultured in LG showed a greater ability to develop to blastocyst stage than embryos cultured in smaller groups, while the blastocyst rate of WOW group was significantly higher than in control. The number of cells/blastocyst in LG was higher than control or WOW, whereas the apoptosis rate per blastocyst was lower. On the other hand, the addition of EGF-ITS significantly improved both parameters compared to the control and resulted in similar embryo quality to LG. In conclusion, the WOW system improved embryo development, while the addition of EGF-ITS improved the embryo quality when smaller groups of embryos were cultured. © 2013 Blackwell Verlag GmbH.

  16. Environmental impacts of cultured meat production.

    Science.gov (United States)

    Tuomisto, Hanna L; de Mattos, M Joost Teixeira

    2011-07-15

    Cultured meat (i.e., meat produced in vitro using tissue engineering techniques) is being developed as a potentially healthier and more efficient alternative to conventional meat. Life cycle assessment (LCA) research method was used for assessing environmental impacts of large-scale cultured meat production. Cyanobacteria hydrolysate was assumed to be used as the nutrient and energy source for muscle cell growth. The results showed that production of 1000 kg cultured meat requires 26-33 GJ energy, 367-521 m(3) water, 190-230 m(2) land, and emits 1900-2240 kg CO(2)-eq GHG emissions. In comparison to conventionally produced European meat, cultured meat involves approximately 7-45% lower energy use (only poultry has lower energy use), 78-96% lower GHG emissions, 99% lower land use, and 82-96% lower water use depending on the product compared. Despite high uncertainty, it is concluded that the overall environmental impacts of cultured meat production are substantially lower than those of conventionally produced meat.

  17. “Fora do Eixo: Estado ou mercado?” Modos de mediação entre produção cultural, política e mercado no Brasil

    Directory of Open Access Journals (Sweden)

    Victoria Irisarri

    2017-01-01

    Full Text Available Forms of contemporary cultural production in Brazil have articulated emerging social forms that put strain and open new questions on the boundaries between cultural production, the political and the market. This paper seeks to analyze the relationships between cul- tural production, the market and the political produced by an emerging artistic-cultural movement, Fora do Eixo. This movement emerged in the last decade, and is characterized by its organization in an articulated collective network and the use of digital technologies for work on cultural production. Drawing upon ethnographic work, this paper analyses some of the meanings that Fora do Eixo gives to certain actions of cultural production, transcending the analytical simplifications to understand the modes of mediation among culture, politics and the market that these movements produce in their own terms.

  18. CHALLENGES IN CROSS CULTURAL ADVERTISING

    OpenAIRE

    Yuni Retnowati

    2016-01-01

    At first, marketing practitioners and academics consider standardized approaches to marketing and advertising strategies in globalization, and then some studies proved that the standardization of advertising across culture is not valid. Therefore, cross cultural advertising takes local culture into account when conveying messages in advertisements. Cross cultural understanding is very important in order to produce successful localized advertising that would reflect the cultural values and nor...

  19. Occurrence of mycotoxin producing fungi in bee pollen.

    Science.gov (United States)

    González, G; Hinojo, M J; Mateo, R; Medina, A; Jiménez, M

    2005-11-15

    The natural mycobiota occurring in bee pollen is studied in the present report with special attention to analyze the incidence of fungal species that are potential producers of mycotoxins. A total of 90 ready-to-eat bee pollen samples were analyzed. Eighty-seven samples were collected in stores placed in different Spanish areas and three were from Buenos Aires (Argentina). The statistical results (ANOVA) showed that yeasts and Penicillium spp. were the predominant fungi. With regard to the potential mycotoxin producing species, Penicillium verrucosum, Aspergillus niger aggregate, Aspergillus carbonarius, Aspergillus ochraceus, Aspergillus flavus, Aspergillus parasiticus and Alternaria spp. were found. The last genus was isolated very frequently. The potential ability for producing ochratoxin A (OTA) and aflatoxins B(1), B(2), G(1) and G(2) was studied by culturing in vitro the isolates followed by analysis of these mycotoxins in culture extracts by HPLC with fluorescent detection. It was found that 100%, 53.3%, 33.3% and 25% of the isolates of A. carbonarius, A. ochraceus, P. verrucosum and A. niger aggregate, respectively, produced OTA. Moreover, 28.6% of the isolates from the A. flavus plus A. parasiticus group were able to produce aflatoxin B(1). Aflatoxin B(2) was detected in only 10% of the cultures. Aflatoxins G(1) and G(2) were not detected in cultures under the assayed conditions. This is the first report carried out on the natural mycobiota occurring in bee pollen in general and on the toxigenic capability of these isolates in particular.

  20. 7 CFR 1206.16 - Producer.

    Science.gov (United States)

    2010-01-01

    ... producing, or causing to be produced, mangos beyond the person's own family use and having value at first... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE MANGO PROMOTION, RESEARCH, AND INFORMATION Mango Promotion, Research, and Information Order Definitions § 1206.16 Producer. Producer means...

  1. The meaning of alcohol to traditional Muscogee Creek Indians.

    Science.gov (United States)

    Wing, D M; Thompson, T

    1996-01-01

    The purpose of this study was to learn the meaning of alcohol to the traditional Muscogee Creek Indians of eastern Oklahoma. Using Leininger's theory of culture care diversity and universality as the theoretical base, the authors conducted interviews of 24 traditional people to elicit both emic and etic meanings of alcohol. The conceptualization of alcohol as a dichotomy of power to do both good and evil emerged as the central theme. Other meanings of alcohol were explicated in relation to five social structure dimensions. The findings suggest culturally competent nursing implications for preserving, accommodating, and repatterning the meaning of alcohol.

  2. Proteolysis produced within biofilms of bacterial isolates from raw milk tankers.

    Science.gov (United States)

    Teh, Koon Hoong; Flint, Steve; Palmer, Jon; Andrewes, Paul; Bremer, Phil; Lindsay, Denise

    2012-06-15

    In this study, six bacterial isolates that produced thermo-resistant enzymes isolated from the internal surfaces of raw milk tankers were evaluated for their ability to produce proteolysis within either single culture biofilms or co-culture biofilms. Biofilms were formed in an in vitro model system that simulated the upper internal surface of a raw milk tanker during a typical summer's day of milk collection in New Zealand. The bacterial isolates were further evaluated for their ability to form biofilms at 25, 30 and 37°C. Mutual and competitive effects were observed in some of the co-culture biofilms, with all isolates being able to form biofilms in either single culture or co-culture at the three temperatures. The proteolysis was also evaluated in both biofilms and corresponding planktonic cultures. The proteolysis per cell decreased as the temperature of incubation (20-37°C) increased. Furthermore, mutualistic interactions in terms of proteolysis were observed when cultures were grown as co-culture biofilms. This is the first study to show that proteolytic enzymes can be produced in biofilms on the internal surfaces of raw milk tankers. This has important implications for the cleaning and the temperature control of raw milk transport tankers. Copyright © 2012 Elsevier B.V. All rights reserved.

  3. The Same but Different: Making Meaning from Modified Texts with Cross-Cultural Themes

    Science.gov (United States)

    Leung, Cynthia B.; Bennett, Susan V.; Gunn, AnnMarie Alberton

    2017-01-01

    Reader response theory provides the framework for the present study that explored literary elements and cultural responses of fifth-grade students to two modified versions of a cross-cultural text, "Homesick: My Own Story" by Jean Fritz. One group of students read the first chapter of the book and another group read a modified basal…

  4. Forming Health Culture of Bachelors of Education by Means of an Academic Course

    Science.gov (United States)

    Asafova, Elena V.; Sazanova, Maria L.

    2016-01-01

    In Russia the system of spreading health-culture among the young generation, the students, has not been formed yet, which makes the paper topical and up-to-date. The young generation is characterized by a low level of education and professional training efficiency in healthy life-style and health culture. It has caused depreciation of the concepts…

  5. Citizenship and cultural diversity in agenda of cultural policies

    Directory of Open Access Journals (Sweden)

    Patricia Silva Dorneles

    2016-01-01

    Full Text Available This is a discussion paper which aims to contribute to the systematization of studies, concepts and practices on cultural policies which have been developed in previous years in Brazil and are orienting cultural actions and public programs in the country, also influencing the Occupational Therapy. Citizenship and Cultural Diversity are concepts under construction and are part of the of the agenda of cultural policies and as well as the reflections and practices of various occupational therapists who are acting in a constant dialogue with the cultural area by means of the formation in cultural management, cultural mapping, programs and grant projects aimed to promote inventive identities, traditional communities, native populations, urban mobility, and cultural networks and exchange initiatives, among others. The article presents the process of this conceptual construction and the constitution of experiences aiming the democratization of the culture in the history of Brazilian cultural public policies, over which are being discussed approach paths and possibilities for Occupational Therapy.

  6. Film Cross-culture Research under the Perspective of Language and Culture

    Institute of Scientific and Technical Information of China (English)

    罗淞译

    2015-01-01

    Language as an important tool of cultural transmission, it can achieve the cross-culture development of film. With the strength of globalization, film cross-culture communication are increasing, and how to enhance the communication of film through language and culture and let more people enjoy the thought expressed in film is one of the most important content for cross-culture development of mant films. Different cultural backgrounds will produce large diversities in watching a same film, so it is helpful for the cross-culture development of film when making good use of culture and language, on the contrary, it will become a hindrance. This article do research on cross-culture development of film under the perspective of language and culture to find out the existing problems in present cross-culture development of film and put forward effective resolution strategy in order to promote certain reference for the internationalization of China’s film industry.

  7. Advertising as a socio-cultural field: discursive component

    Directory of Open Access Journals (Sweden)

    O. I. Gerus

    2016-10-01

    Full Text Available Using the theory of social fields by P. Bourdieu the article describes the features of the advertising as a symbolic cultural field functioning. Confined to the locality of its actions, advertising creates cultural forms, images, facilities, value­based judgments that become widespread, claiming the role of human behavior that must be followed. The author defines advertising as a symbolic cultural field. To analyse the advertising field and determine the impact of advertising on learning and the formation of certain values and cultural patterns the author justifies application of critical discourse analysis (CDA. The meaning of notions «advertising discourse» and «discourses of advertising» are being exposed and delineated. The author propose and test research methodology of development and impact of advertising discourse on advertising consumers. The types of value discourses prevalent in the field of commercial advertising were identified. In the study, the author found that advertising discourses are dynamic entities that are changed according to time and the state of the society in which they operate. In particular, the discourses produced in advertising influences social context ­ developments in society, the dominant values and attitudes.

  8. Politics of Meaning in Mathematics Education

    DEFF Research Database (Denmark)

    Skovsmose, Ole

    2016-01-01

    By a politics of meaning I refer to the social, economic, cultural and religious conditions for experiencing meaning. I refer as well to the layers of visons, assumptions, presumptions and preconceptions that might construct something as being meaningful. By addressing different politics of meani...... of such factors. Politics of meaning can be analysed with reference to sexism, racism, instrumentalism, the school mathematics tradition, critical mathematics education, and the banality of expertise....

  9. Meaning construction in remembering

    DEFF Research Database (Denmark)

    Wagoner, Brady

    2011-01-01

    F.C. Bartlett and L.S.Vygotsky were two seminal figures in the psychological study of remembering. Both emphasized the role of meaning and imagination in this process. Bartlett did this by showing the systematic and holistic changes that ensue when cultural material is repeatedly reproduced outsi...

  10. A portrait oil lamp from Pontes: Possible interpretations and meanings within early Byzantine visual culture

    Directory of Open Access Journals (Sweden)

    Petković Sofija

    2015-01-01

    Full Text Available The subject of this paper is a fragmented oil-lamp, discovered at Pontes (east Serbia dated to the 6th century, whose handle ending is shaped as a woman’s head. The question posed in this paper is whether the image of this woman could be identified as a portrait of some particular person or if it is just as a pictorial sign with some complicated symbolic meaning. The suggested identification alludes to the image of some of the empresses from the second half of the 6th century. [Projekat Ministarstva nauke Republike Srbije, br. 177007: Romanisation, urbanisation and transformation of urban centres of civil, military and residential character in Roman provinces in the territory of Serbia i br. 47018: Viminacium, Roman city and military camp - research of the material and non material culture of inhabitants by using the modern technologies of remote detection, geophysics, GIS, digitalization and3D visualization

  11. The implied producer investigating an emergent typology in participatory culture

    DEFF Research Database (Denmark)

    Søndergaard, Morten

    2012-01-01

    Biennales, that we are not 'just' dealing with a 'new' genre or style within the art category; on the other hand we are not dealing with a pure commercial culture either (the abstract notion of 'the user' has its limits); what is becoming evident is that the 'implicit' roles of the participatory 'actors...

  12. Bourlingue culturelle (Cultural Rambling).

    Science.gov (United States)

    Thieriot, Jacques

    1980-01-01

    Reflects on the meaning of cultural education and activity in France as inspired by the need to establish a new form of cultural dialogue between France and the people of other countries. Problems related to financing cultural activities, reaching various types of audience, and methodology are discussed. (AMH)

  13. KEEPING CULTURAL GENES ALIVE

    Institute of Scientific and Technical Information of China (English)

    Bai Shi

    2012-01-01

    China's contemporary culture and the protection of its diverse cultural heritage have become some of the most talked about issues today.Cultural prosperity was put forward as an important objective of the Central Government's national development strategy last year.However,the industrialization and commercialization of China's culture have been both criticized and celebrated.Many scholars believe industrialization and enormous government investment may not be the best means to protect intangible cultural heritage (ICH).

  14. A política cultural: regulação estatal e mecenato privado

    Directory of Open Access Journals (Sweden)

    Maria Arminda do Nascimento Arruda

    2003-11-01

    Full Text Available O artigo traça as diretrizes da política cultural implementada durante o segundo governo FHC: leis de incentivo fiscal, financiamento privado e regulação estatal. A ação cultural do governo atribuiu ao Estado o papel de intermediário no processo de financiamento, avalizando relações entre produtores culturais e agentes econômicos. Em lugar de um típico mecenato privado, instituiu-se uma nova modalidade de promoção cultural. A expansão das iniciativas nos diversos ramos da criação cultural não significou, necessariamente, a renovação das linguagens.This paper traces the guidelines of the cultural policies prevailing during Fernando Henrique Cardoso's second term of government: fiscal incentive legislation, private funding and state regulation. The government's cultural action designed the state as regulator, acting as "go-between" towards the cultural producers and the new economic agents. Instead of a typical private patronage model, the state instituted a new kind of cultural promotion. The expansion of initiatives in every domain of cultural creation did not mean, necessarily, the renewal of languages.

  15. Cultural context and school counseling: Cultural sensitivity to advocate for social justice

    Directory of Open Access Journals (Sweden)

    Moshé Tatar

    2008-06-01

    Full Text Available In this paper the relevance of understanding the different meanings of culture in the counseling profession is presented. Two approaches to the concept of culture as they relate to counseling are suggested: the first approach stresses the organisational culture of the institution where the counselor works; the second —the multicultural approach— calls for the complex recognition of the variety of ethnic cultural backgrounds of those involved in the counseling situation. Professional practices are analysed as means for the reinforcement of present conditions or as ways for changing them. The concepts of empowerment of and advocacy for our clients are put forward as main components in the challenging new roles of the counseling profession. Implications for counselors are suggested.

  16. Forecasting Error Calculation with Mean Absolute Deviation and Mean Absolute Percentage Error

    Science.gov (United States)

    Khair, Ummul; Fahmi, Hasanul; Hakim, Sarudin Al; Rahim, Robbi

    2017-12-01

    Prediction using a forecasting method is one of the most important things for an organization, the selection of appropriate forecasting methods is also important but the percentage error of a method is more important in order for decision makers to adopt the right culture, the use of the Mean Absolute Deviation and Mean Absolute Percentage Error to calculate the percentage of mistakes in the least square method resulted in a percentage of 9.77% and it was decided that the least square method be worked for time series and trend data.

  17. Relationships between food producers and retail chains seen as shared meanings

    DEFF Research Database (Denmark)

    Skytte, Hans

    This paper presents a new theory on relationships between producers and retail chains. This theory is a result of a project which investigated the cooperation between Danish abattoirs and food processors, and retail chains in four countries. The new theory's main point is that relationships betwe...

  18. Identify Web-page Content meaning using Knowledge based System for Dual Meaning Words

    OpenAIRE

    Sinha, Sukanta; Dattagupta, Rana; Mukhopadhyay, Debajyoti

    2012-01-01

    Meaning of Web-page content plays a big role while produced a search result from a search engine. Most of the cases Web-page meaning stored in title or meta-tag area but those meanings do not always match with Web-page content. To overcome this situation we need to go through the Web-page content to identify the Web-page meaning. In such cases, where Webpage content holds dual meaning words that time it is really difficult to identify the meaning of the Web-page. In this paper, we are introdu...

  19. Meanings of the Home and Living

    Directory of Open Access Journals (Sweden)

    Verônica Gonçalves Azeredo

    2016-10-01

    Full Text Available This study aims at making considerations about what homes mean to their residents, an an approach related to the socio-cultural processes underlying its spatiality. The article is essentially theoretical and based on the literature. Following this methodology, the research was conducted in order to explore ethnographic sources focused on the anthropology of space. Reflections were guided by the notion of homes as well as the relations established in them, with emphasis on the intersection between the territorial, architectural, cultural, sociodemographic and psychological aspects. In addition to conceptual abstractions, the investigation aims to understand the meaning of and in space, the importance of homes, and the relationships established between them and their residents.

  20. Laboratory Workflow Analysis of Culture of Periprosthetic Tissues in Blood Culture Bottles.

    Science.gov (United States)

    Peel, Trisha N; Sedarski, John A; Dylla, Brenda L; Shannon, Samantha K; Amirahmadi, Fazlollaah; Hughes, John G; Cheng, Allen C; Patel, Robin

    2017-09-01

    Culture of periprosthetic tissue specimens in blood culture bottles is more sensitive than conventional techniques, but the impact on laboratory workflow has yet to be addressed. Herein, we examined the impact of culture of periprosthetic tissues in blood culture bottles on laboratory workflow and cost. The workflow was process mapped, decision tree models were constructed using probabilities of positive and negative cultures drawn from our published study (T. N. Peel, B. L. Dylla, J. G. Hughes, D. T. Lynch, K. E. Greenwood-Quaintance, A. C. Cheng, J. N. Mandrekar, and R. Patel, mBio 7:e01776-15, 2016, https://doi.org/10.1128/mBio.01776-15), and the processing times and resource costs from the laboratory staff time viewpoint were used to compare periprosthetic tissues culture processes using conventional techniques with culture in blood culture bottles. Sensitivity analysis was performed using various rates of positive cultures. Annualized labor savings were estimated based on salary costs from the U.S. Labor Bureau for Laboratory staff. The model demonstrated a 60.1% reduction in mean total staff time with the adoption of tissue inoculation into blood culture bottles compared to conventional techniques (mean ± standard deviation, 30.7 ± 27.6 versus 77.0 ± 35.3 h per month, respectively; P < 0.001). The estimated annualized labor cost savings of culture using blood culture bottles was $10,876.83 (±$337.16). Sensitivity analysis was performed using various rates of culture positivity (5 to 50%). Culture in blood culture bottles was cost-effective, based on the estimated labor cost savings of $2,132.71 for each percent increase in test accuracy. In conclusion, culture of periprosthetic tissue in blood culture bottles is not only more accurate than but is also cost-saving compared to conventional culture methods. Copyright © 2017 American Society for Microbiology.

  1. Culturing the Unculturable: Human Coronavirus HKU1 Infects, Replicates, and Produces Progeny Virions in Human Ciliated Airway Epithelial Cell Cultures

    NARCIS (Netherlands)

    Pyrc, Krzysztof; Sims, Amy C.; Dijkman, Ronald; Jebbink, Maarten; Long, Casey; Deming, Damon; Donaldson, Eric; Vabret, Astrid; Baric, Ralph; van der Hoek, Lia; Pickles, Raymond

    2010-01-01

    Culturing newly identified human lung pathogens from clinical sample isolates can represent a daunting task, with problems ranging from low levels of pathogens to the presence of growth suppressive factors in the specimens, compounded by the lack of a suitable tissue culture system. However, it is

  2. Intertextuality as A Cultural Interaction Mechanism and Socio-Cultural Phenomenon

    Directory of Open Access Journals (Sweden)

    E P Shiniev

    2010-03-01

    Full Text Available The article focuses on intertextuality as a concept associated with the tradition of postmodernism textology and a phenomenon of text interaction with the semiotic cultural environment. The concept of intertextuality emerges as an essential condition for the construction of meaning which includes the dialogical characteristics of the polylogue culture field. The author examines the evolution of intertextuality since M.M. Bakhtin up to contemporary theoreticians of postmodernism. The concept of intertextuality is the prominent characteristics of the contemporary «post» culture and the major channel of inter-cultural diffusion.

  3. Microalgae culture collection, 1986-1987

    Energy Technology Data Exchange (ETDEWEB)

    Barclay, W.; Johansen, J.; Chelf, P.; Nagle, N.; Roessler, P.; Lemke, P.

    1986-12-01

    The SERI Microalgae Culture Collection provides a repository for strains identified or developed for mass culture biomass production and makes these strains readily available to the research community. The strains in the collection have been selected for their potential in biomass fuel applications, and many produce significant quantities of cellular storage lipids. All of the newly added strains have been recently isolated by SERI and its subcontractors in organized screening programs. Many have been tested in outdoor mass culture systems, and several have demonstrated excellent performance as biomass producers. The strains added to the collection this year have been isolated from inland saline waters and marine waters. We believe that the strains in this collection can provide a source of extremely useful organisms, both for laboratory experimentation and for mass culture research. Most of the strains are currently nonaxenic. Again this year, cultures will be shipped free of charge to interested researchers. An important function of the culture collection catalog, in addition to listing the available strains, is to provide culture and performance data for each of the organisms. By collecting a summary of the requirements and characteristics of these organisms, we hope to allow requestors of cultures to begin productive research with a minimum of preliminary work on culture techniques.

  4. Evaluation of the Synergistic Effect of Mixed Cultures of White-Rot Fungus Pleurotus ostreatus and Biosurfactant-Producing Bacteria on DDT Biodegradation.

    Science.gov (United States)

    Purnomo, Adi Setyo; Ashari, Khoirul; Hermansyah, Farizha Triyogi

    2017-07-28

    DDT (1,1,1-trichloro-2,2- bis (4-chlorophenyl) ethane) is one of the organic synthetic pesticides that has many negative effects for human health and the environment. The purpose of this study was to investigate the synergistic effect of mixed cutures of white-rot fungus, Pleurotus ostreatus , and biosurfactant-producing bacteria, Pseudomonas aeruginosa and Bacillus subtilis , on DDT biodegradation. Bacteria were added into the P. ostreatus culture (mycelial wet weight on average by 8.53 g) in concentrations of 1, 3, 5, and 10 ml (1 ml ≈ 1.25 × 10 9 bacteria cells/ml culture). DDT was degraded to approximately 19% by P. ostreatus during the 7-day incubation period. The principal result of this study was that the addition of 3 ml of P. aeruginosa into P. ostreatus culture gave the highest DDT degradation rate (approximately 86%) during the 7-day incubation period. This mixed culture combination of the fungus and bacteria also gave the best ratio of optimization of 1.91. DDD (1,1-dichloro-2,2- bis (4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2- bis (4-chlorophenyl) ethylene), and DDMU (1-chloro-2,2- bis (4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by P. ostreatus and P. aeruginosa . The results of this study indicate that P. aeruginosa has a synergistic relationship with P. ostreatus and can be used to optimize the degradation of DDT by P. ostreatus .

  5. What Is so "Cultural" About Cultural Entrepreneurship

    Directory of Open Access Journals (Sweden)

    Staffan Albinsson

    2017-04-01

    Full Text Available The term “cultural entrepreneurship” has been increasingly used during the new millennium, mirroring the rapidly growing importance of the “quaternary sector of the economy,” i.e. knowledge-based industries, including culture. Exploration of the literature in which the term “cultural entrepreneurship” is used does not bring a solid, clear-cut, and unambiguous understanding of its definition or meaning. The aim of this paper is to present various uses of the concept and to bring about some clarity in how the concept can be understood. Two overarching uses of the cultural entrepreneurship concept have been found: 1. the anthropologist’s and institutional economist’s use, which indicates the dynamic development of intangible cultural features such as symbols, myths, languages, beliefs, values, norms, rituals, and attitudes in and between societies, and 2. the arts development use, which indicates the dynamic development of cultural services, tangible goods, and individual or collective career promotion. Most authors use a cultural entrepreneurship concept without defining it. Authors could apply more precise definitions by using a quadruple bottom-line framework to position themselves in the nexus of financial, social, artistic, and cultural perspectives.

  6. Therapeutic potential of secondary metabolites produced in the hairy roots cultures

    Directory of Open Access Journals (Sweden)

    Tomasz Kowalczyk

    2015-05-01

    Full Text Available Plants have always been a source of many valuable substances for humans. Growing advancement of methods of modern biotechnology, combined with genetic engineering techniques, gradually increase the variety of compounds obtained, the number of plant species used and the production efficiency. Consequently, there is an undebatable interest in biotechnological production of such compounds, especially those pharmacologically active, that can be used in treatment of neoplastic, viral, and many other types of diseases. Most of these compounds represent a diverse group of secondary metabolites. One of the effective ways of obtaining such molecules is the utilization of hairy roots cultures. The advantages of such systems make them an attractive method of obtaining important plant-derived compounds, creating an interesting alternative to other methods, including the cell suspension cultures or expensive chemical syntheses.

  7. Microalgae Culture Collection, 1985-1986

    Energy Technology Data Exchange (ETDEWEB)

    1986-01-01

    The SERI Microalgae Culture Collection was established in support of the US Department of Energy's Biofuels Program to provide a repository for strains identified or developed for mass culture biomass production and to make these strains readily available to the research community. The strains in the collection have been selected for their potential in biomass fuel applications, and many produce significant quantities of cellular storage lipids. The Culture Collection Catalog lists 20 strains of ten species. Many have been tested in outdoor mass culture systems, and several have demonstrated excellent performance as biomass producers, with yields of up to 40 grams of organic matter per square meter per day. The majority of strains added to the collection this year have been isolated from inland saline waters, although marine species are included as well. We believe that the strains in this collection can provide a source of extremely useful organisms, both for laboratory experimentation and for mass culture research. 98 refs., 31 figs., 52 tabs.

  8. The conducting shell stellarator: A simple means for producing complicated fields

    International Nuclear Information System (INIS)

    Sheffield, G.V.

    1997-01-01

    One of the main characteristics of stellarators, both helical and modular, is that their coil sets must take difficult shapes in order to produce the complicated stellarator magnetic fields. The complex coil shapes make fabrication difficult and costly compared to say the toroidal field, TF, coil set of a tokamak. The conducting shell stellarator, CSS, configuration described in this report shows that complicated stellarator fields can be produced by inducing eddy currents in a conducting shell from a simple TF coil set (a field that varies like 1/R). This technique is applicable not only to a pulsed system at room or cryogenic temperatures, but can be implemented for a superconducting TF with a superconducting shell in a stellarator reactor. The CSS has the added benefit that within this device the metallic shell which can be made up of discrete plates can be changed out and replaced with new plates to create a different stellarator configuration within the same TF coil set. The work of creating the complicated magnetics is done by the passive conductor reshaping the simple TF field

  9. Ethnographic exercises as activities in public space: Social Occupational Therapy in art, culture and politics

    Directory of Open Access Journals (Sweden)

    Debora Galvani

    2016-10-01

    Full Text Available Ethnographic exercises are discussed - as proposed by the Metuia Project/ USP between 2007 and 2013 - as an activity able to enhance the recognition of the compound, plural and sometimes contradictory knowledge, but produced creatively in the intellectual and social do, in the interaction among students, occupational therapists, researchers and homeless people. It starts from the need to develop an understanding of the significant activities of artists working in the public spaces in São Paulo, as it persists as a plurality of meanings that the street acquires amid disputes of interests and cultural tensions, but also interconnections and creativity. The itinerant life and social areas’ characteristics, combined with reflections of urban anthropology and ethnographic research favored the theoretical and practical teaching in dialogic territorial shares of social occupational therapy. This article is the result of reflections built from the research Circuits and religious practices in life trajectories of adult homeless people in city of São Paulo, associated with university extension project linked to Metuia Project/USP, called Point meeting and culture: social networks, culture and social occupational therapy. In conclusion, on the one hand, there is need for renewed reflection about the occupational therapist’s place, considering the asymmetries of the relations in the construction of knowledge. On the other hand, it indicates that the produced activities, necessarily, in dialogical relations, only share meanings when inserted into the experience of the difference in consistent proposals with its own plasticity and in the middle of specific social and cultural contexts.

  10. Extended-spectrum beta-lactamase-producing bacteria are not detected in supragingival plaque samples from human fecal carriers of ESBL-producing Enterobacteriaceae

    Directory of Open Access Journals (Sweden)

    Arne Søraas

    2014-08-01

    Full Text Available Background: The prevalence of infections caused by Cefotaximase-Munich (CTX-M-type extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E has rapidly increased during the past 15 years. Enterobacteriaceae are commonly found in the gastrointestinal tract and long-term intestinal carriage is considered important for the spread of ESBL and as a source of clinical infections. Oral biofilm such as supragingival plaque is known to contain numerous antibiotic resistance determinants and may also represent a poorly investigated site for ESBL carriage and further spread. Objective: To investigate possible carriage of ESBL-producing bacteria in supragingival plaque of known fecal carriers of these bacteria. Design: We screened for the presence of aerobic and anaerobic ESBL-producing bacteria and blaCTX-M in supragingival plaque samples from healthy human adults with culture-verified fecal carriage of CTX-M-producing Escherichia coli. The presence or absence of Enterobacteriaceae and ESBL-producing bacteria in plaque samples was evaluated using culture-based methods and consensus CTX-M PCR. Results: Oral samples were obtained from 17 participants with known previous carriage of ESBL-producing E. coli. No ESBL-producing bacteria or ESBL genes were detected using culture-based and molecular methods. One colony of Rahnella aquatilis harboring the class A ESBL gene bla RAHN-1/2 was identified in an oral sample from one of the participants. Conclusion: This pilot study supports the notion that the presence of CTX-M-producing bacteria is uncommon in oral plaque of healthy human adult fecal carriers. Due to the limited number of persons tested, a low prevalence of oral ESBL-carriage in healthy adults or carriage in selected groups of patients cannot be excluded. To our knowledge, this is the first description of an R. aquatilis with the RAHN-1/2 gene in the oral cavity.

  11. Influence of culture medium supplementation of tobacco NT1 cell suspension cultures on the N-glycosylation of human secreted alkaline phosphatase.

    Science.gov (United States)

    Becerra-Arteaga, Alejandro; Shuler, Michael L

    2007-08-15

    We report for the first time that culture conditions, specifically culture medium supplementation with nucleotide-sugar precursors, can alter significantly the N-linked glycosylation of a recombinant protein in plant cell culture. Human secreted alkaline phosphatase produced in tobacco NT1 cell suspension cultures was used as a model system. Plant cell cultures were supplemented with ammonia (30 mM), galactose (1 mM) and glucosamine (10 mM) to improve the extent of N-linked glycosylation. The highest levels of cell density and active extracellular SEAP in supplemented cultures were on average 260 g/L and 0.21 U/mL, respectively, compared to 340 g/L and 0.4 U/mL in unsupplemented cultures. The glycosylation profile of SEAP produced in supplemented cultures was determined via electrospray ionization mass spectrometry with precursor ion scanning and compared to that of SEAP produced in unsupplemented cultures. In supplemented and unsupplemented cultures, two biantennary complex-type structures terminated with one or two N-acetylglucosamines and one paucimannosidic glycan structure comprised about 85% of the SEAP glycan pool. These three structures contained plant-specific xylose and fucose residues and their relative abundances were affected by each supplement. High mannose structures (6-9 mannose residues) accounted for the remaining 15% glycans in all cases. The highest proportion (approximately 66%) of a single complex-type biantennary glycan structure terminated in both antennae by N- acetylglucosamine was obtained with glucosamine supplementation versus only 6% in unsupplemented medium. This structure is amenable for in vitro modification to yield a more human-like glycan and could serve as a route to plant cell culture produced therapeutic glycoproteins. (c) 2007 Wiley Periodicals, Inc.

  12. Co-culture of neural crest stem cells (NCSC and insulin producing beta-TC6 cells results in cadherin junctions and protection against cytokine-induced beta-cell death.

    Directory of Open Access Journals (Sweden)

    Anongnad Ngamjariyawat

    Full Text Available PURPOSE: Transplantation of pancreatic islets to Type 1 diabetes patients is hampered by inflammatory reactions at the transplantation site leading to dysfunction and death of insulin producing beta-cells. Recently we have shown that co-transplantation of neural crest stem cells (NCSCs together with the islet cells improves transplantation outcome. The aim of the present investigation was to describe in vitro interactions between NCSCs and insulin producing beta-TC6 cells that may mediate protection against cytokine-induced beta-cell death. PROCEDURES: Beta-TC6 and NCSC cells were cultured either alone or together, and either with or without cell culture inserts. The cultures were then exposed to the pro-inflammatory cytokines IL-1β and IFN-γ for 48 hours followed by analysis of cell death rates (flow cytometry, nitrite production (Griess reagent, protein localization (immunofluorescence and protein phosphorylation (flow cytometry. RESULTS: We observed that beta-TC6 cells co-cultured with NCSCs were protected against cytokine-induced cell death, but not when separated by cell culture inserts. This occurred in parallel with (i augmented production of nitrite from beta-TC6 cells, indicating that increased cell survival allows a sustained production of nitric oxide; (ii NCSC-derived laminin production; (iii decreased phospho-FAK staining in beta-TC6 cell focal adhesions, and (iv decreased beta-TC6 cell phosphorylation of ERK(T202/Y204, FAK(Y397 and FAK(Y576. Furthermore, co-culture also resulted in cadherin and beta-catenin accumulations at the NCSC/beta-TC6 cell junctions. Finally, the gap junction inhibitor carbenoxolone did not affect cytokine-induced beta-cell death during co-culture with NCSCs. CONCLUSION: In summary, direct contacts, but not soluble factors, promote improved beta-TC6 viability when co-cultured with NCSCs. We hypothesize that cadherin junctions between NCSC and beta-TC6 cells promote powerful signals that maintain beta

  13. Folk national culture as a means of forming norms of communication in childhood

    Directory of Open Access Journals (Sweden)

    Chernushevich V. A.

    2016-06-01

    Full Text Available The best carriers of playing culture are children, who possess and enjoy it. Destroyed social kids’ structures, territorial kids’ associations (family, yard, village, street communities of children interrupted generally the process of culture transmission, reproduction and passing of communication tradition. And there is a need in social-state “revivification” (recovering folk games list and its’ players, enough for folk games reproduction process. Folk game includes particular properties of relations on the levels of physical and emotional, vocal interaction, imagery-symbolic filling, special features of clothes (all aspects of communication that constitute features of national culture of the nation and make from the nation the community of people very special and different from other communities and nations. Studying of correctional possibilities of folk games within the frames of playing agendas showed that their psychological and emotional resources provide the conditions for adoption by children the norms of communication.

  14. Re-interpreting cultural values: Tajikistani students abroad

    Directory of Open Access Journals (Sweden)

    Nazira Sodatsayrova

    2017-11-01

    Full Text Available This article looks at the mobility of Tajikistani students and how they negotiate their place in an increasing globalised world, where national boundaries are becoming more navigable. It explores how structural and cultural factors intersect and influence young people’s choices as they re-shape their connections to their homeland, and re-negotiate cultural meanings in the new geographical and cultural contexts they find themselves in. This inquiry uses the concept of translocality which frames the negotiation of cultural meanings in a distant geographical and (host cultural space by acknowledging the place of the “local connection” within international mobility. It does this by examining students’ perceptions of two cultural concepts: nomus or “keeping the family name high in the community” and kase shudan or “being the shaper of one’s own destiny.” Drawing on Stephen and Storey’s conceptualisation of culture (and the local notions of agency the article explores how continuity and contestation are juxtaposed and brought to bear on the new meaning-making by the students, against the national and international agendas that define student mobility choices. Using qualitative methodology, the researchers engaged with Tajikistani research participants in England and Japan, and looked at what those decisions about mobility mean for the individual students, locating them in a translocal (as opposed to a transnational space. The article finds that contrary to the usual expectations of immigrants adhering more closely to cultural values (for fear of diluting them in a new setting, while the local cultures keep evolving, these students re-interpreted traditional values to take account of their new settings and their exposure to new cultures and spaces and sought to expand meanings rather than constrain them in traditional moulds.

  15. Producing Films, Producing Meanings: Some Theoretical Reflections

    Directory of Open Access Journals (Sweden)

    Giorgio Grignaffini

    2015-01-01

    Full Text Available L'articolo si pone l'obiettivo di stimolare una maggiore attenzione da parte della semiotica del cinema alle pratiche produttive e ai materiali preparatori del film, finora poco studiati rispetto alle dinamiche che i testi stessi attivano sulla ricezione. Tale proposta di allargamento del campo di ricerca, viene approfondita nelle sue premesse teoriche in due momenti. Dapprima viene contestualizzata rispetto alla pratica dell'analisi semiotica "tradizionale"; poi, utilizzando gli strumenti della sociosemiotica elaborata da Eric Landowski, in particolare sfruttando il concetto di "aggiustamento" sviluppato nel quadro della riflessione sulle modalità di interazione, vengono evidenziati campi di applicazione (ad es. il lavoro del regista con l’attore e possibili vantaggi euristici.

  16. Fast growing aspens in the development of a plant micropropagation system based on plant-produced ethylene action

    International Nuclear Information System (INIS)

    Žiauka, Jonas; Kuusienė, Sigutė; Šilininkas, Mindaugas

    2013-01-01

    Representatives of the genus Populus (poplars), such as Populus tremula L. (European aspen) and its fast-growing hybrids, are recognized as being among the most suitable tree species for short rotation coppicing in Northern Europe. Several technologies have been developed for fast propagation of selected aspen genotypes, including laboratory (in vitro) micropropagation, which is usually based on the action of exogenous plant hormones. Seeking to minimize the use of the latter, the present study was designed to test if the conditions suitable for increased accumulation of plant-produced gas, including the gaseous plant hormone ethylene, inside a culture vessel could contribute to commercially desirable changes in aspen development. Shoot cultures of several European and hybrid (Populus tremuloides Michx. × P. tremula) aspen genotypes were studied using two different types of culture vessels: tightly sealed Petri dishes (15 × 54 mm) designed to provide restricted gas exchange (RGE) conditions, and capped (but not sealed) test tubes (150 × 18 mm) providing control conditions. Under RGE conditions, not only the positive impact of the ethylene precursors 1-aminocyclopropane-1-carboxylic-acid (ACC) and ethephon on shoot proliferation was demonstrated but also a several-fold increase, compared to the control conditions, in the mean shoot number per explant was recorded even on the hormone-free nutrient medium. Moreover, the shoots developed under RGE conditions were distinguished by superior rooting ability in the subsequent culture. These results suggest that a plant micropropagation system based on the action of plant-produced ethylene rather than of exogenous hormones is possible. -- Highlights: ► Aspen in vitro cultures were grown in different vessels. ► Small-volume vessels were used for restriction of gas exchange. ► Aspen explants produced most shoots in small-volume vessels. ► Shoot proliferation was increased due to explant response to ethylene.

  17. The value of cultures to modern microbiology.

    Science.gov (United States)

    Austin, Brian

    2017-10-01

    Since the late nineteenth century, pure cultures have been regarded as the cornerstone of bacteriology. However, not all bacteria will multiply sufficiently to produce visible colonies on solid media; some cells will produce micro-colonies that are invisible to the naked eye. Moreover, the proportion of culturable cells that produce visible growth will vary according to the species and the state of the cells-are they actively growing or comparatively inactive? The latter have a poorer rate of recovery in terms of cultivability. It is unclear whether or not an individual colony is always derived from a single cell; it is possible that organisms in close proximity to each other may multiply and come together to produce single colonies. Then, the resultant growth will most certainly be derived from more than one initial cell. Although it is generally assumed that streaking and re-streaking on fresh media will purify any culture, there is evidence for microbial consortia interacting to form what appear to be single pure cultures. As so-called pure cultures underpin traditional microbiology, it is relevant to understand that the culture does not necessarily contain clones of identical bacteria, but that there may be variation in the genetic potential of the component cells, i.e. the cells are not homogeneous. Certainly, many bacteria change rapidly upon culturing, with some becoming bigger and less active. It is difficult to be sure if these changes reflect a loss or change of DNA or whether standard culturing methods select faster growing cells that are effectively not representative of the environment from which they were derived. These concepts are reviewed with an emphasis on bacterial fish pathogens.

  18. Benner's remnants: culture, tradition and everyday understanding.

    Science.gov (United States)

    Paley, John

    2002-06-01

    Benner's account of meaning and embodiment in nursing depends on a theory which she has never fully articulated, although she makes numerous allusions to it. Behind the background of shared meanings hovers something called 'culture', which provides each individual with meaning, determines what counts as real for her, and actively hands down interpretation-laden practices. This view is based, Benner claims, on the Heideggerian assumption that the meaning and organization of a culture precedes individual meaning-giving activity. I explore Benner's implicit view of culture, drawing on her published work over 15 years, and offer an appraisal of it. In doing so, I attempt to make sense of some rather strange remarks Benner has recently made about 'remnants' of Cartesian and Kantian thinking being found in the everyday understandings of people with asthma. The concept of culture is developed with reference to both Benner's own work and that of the anthropologist, Clifford Geertz, whose work she frequently cites. Having identified the principal tenets of what we might conveniently call the Benner-Geertz theory, I proceed to interrogate the theory, using the recent anthropological literature -- and, in particular, materialist attacks on the idea of culture as a system of meanings -- in order to cast doubt on it. I also review, very briefly, an alternative way of understanding 'culture', which is not vulnerable to the same criticisms. Benner's implicit theory of culture is revealed, somewhat ironically, as an inverted form of Cartesian dualism. Its intellectual provenance is not Heidegger, who appears to reject it, but the sort of American sociology associated with Talcott Parsons. As a corollary, it is suggested that Benner's 'remnants' analogy cannot be justified, and that the idea of Cartesian and Kantian concepts permeating Western culture, infecting both the providers and receivers of health care, is a myth.

  19. CULTURAL PHENOMENA AND PROCESSES IN CONTEMPORARY SOCIETY – DETERMINANTS OF CULTURAL POLICIES

    Directory of Open Access Journals (Sweden)

    ANDREEA MIHAELA NITA

    2012-05-01

    Full Text Available Cultural phenomena and processes in the contemporary society, influenced by the social development models and by the fact that the constitutive elements of the culture have become the decisive factors of social change, are the determinants of the cultural policies. They are centered on the active process of cultural globalization that emphasizes besides the assimilation of the European principles, also the preservation of the cultural dialog without identity loss. Contemporary culture cannot be appreciated unless we know the main processes and phenomena that lately have generated impressive changes in the area of technology and means of communication. Due to these transformations we witness a change of the cultural paradigms, a mutation of values.

  20. Culture, cross-role consistency, and adjustment: testing trait and cultural psychology perspectives.

    Science.gov (United States)

    Church, A Timothy; Anderson-Harumi, Cheryl A; del Prado, Alicia M; Curtis, Guy J; Tanaka-Matsumi, Junko; Valdez Medina, José L; Mastor, Khairul A; White, Fiona A; Miramontes, Lilia A; Katigbak, Marcia S

    2008-09-01

    Trait and cultural psychology perspectives on cross-role consistency and its relation to adjustment were examined in 2 individualistic cultures, the United States (N=231) and Australia (N=195), and 4 collectivistic cultures, Mexico (N=199), the Philippines (N=195), Malaysia (N=217), and Japan (N=180). Cross-role consistency in trait ratings was evident in all cultures, supporting trait perspectives. Cultural comparisons of mean consistency provided support for cultural psychology perspectives as applied to East Asian cultures (i.e., Japan) but not collectivistic cultures more generally. Some but not all of the hypothesized predictors of consistency were supported across cultures. Cross-role consistency predicted aspects of adjustment in all cultures, but prediction was most reliable in the U.S. sample and weakest in the Japanese sample. Alternative constructs proposed by cultural psychologists--personality coherence, social appraisal, and relationship harmony--predicted adjustment in all cultures but were not, as hypothesized, better predictors of adjustment in collectivistic cultures than in individualistic cultures.

  1. Construction Meaning of Mixed Marriages for Indonesian Muslim Women

    Directory of Open Access Journals (Sweden)

    Zikri Fachrul Nurhadi

    2016-07-01

    Full Text Available The background of this problem is the increasing number of Indonesian citizens who perform mixed marriages, especially women who are married to foreign nationals. This, resulted in the problem starts from differences of religion or belief, culture and lifestyle are different. The purpose of this study is to find and explain the motives, meaning and experience of Indonesian Muslim women as perpetrators of intermarriage. This research method using the phenomenological method that focuses on the study of meaning in everyday life from the perspective of those who experience it. Data collection techniques used participant observation, interview and documentation study. Subjects were Indonesian Muslim women aged 30-40 years, were married to foreign nationals by purposive sampling technique. The results showed that mixed marriages have a motive "because" that is the motive trauma and interest, while the motive "for" consists of the dream motive, worship and repair descent. Likewise intermarriage experience demonstrated mutual culturally adjust, adapt to a multicultural, open and romantic attitude. While the meaning of marriage is an interesting mix, happy, merging two cultures, respect for differences, mutual understanding, complex, beautiful. Construction of meaning formed that a mixed marriage is a marriage of attractive, beautiful, full of challenges in the face of differences in terms of culture, habits and mindset in running family life.

  2. Radiosensitivity in cultured human fibroblasts

    International Nuclear Information System (INIS)

    Cox, R.; Masson, W.K.

    1980-01-01

    Caution is urged in the use of freshly isolated cultures of human diploid fibroblasts for quantitative studies of radiosensitivity. The distribution of x ray sensitivities of 'normal' human fibroblast cultures of foetal origin (10 subjects, skin or lung biopsy) and post-foetal origin (34 subjects, skin biopsy) are compared with the distribution in 12 patients with ataxia telangiectasia (probability of including any one of these in a normal post-foetal distribution is 0.01%). Cultures from nominally normal subjects showed a broad distribution of D 0 range of 98 +- 160 rad and assuming normal distribution, a mean +- one standard deviation of 122 +- 17 rad. Mean D 0 values for foetal origin cultures were 117 +- 12; values for post-foetal cultures D 0 were 124 +- 18. No systematic variation in D 0 was observed for age of donor, number of cell divisions in culture or for cloning efficiency. For ataxia telangiectasia D 0 values were 46 +- 7 rad. (U.K.)

  3. Volatiles produced by Staphylococcus xylosus and Staphylococcus carnosus during growth in sausage minces

    DEFF Research Database (Denmark)

    Stahnke, Marie Louise Heller

    1999-01-01

    Aseptic model minces were inoculated with commercial samples of either Staphylococcus xylosus or Staphylococcus carnosus. Volatiles produced by the cultures were collected during growth by diffusive sampling onto adsorbent traps, identified by thermal desorption-gas chromatography-mass spectrometry...... and quantified by thermal desorption-gas chromatography-flame ionisation. The data were analysed by principal component analysis. The study showed that both starter cultures produced a large number of volatiles in concentrations of sensory importance. Almost all of the major volatiles resulted from amino acid...... degradation, suggesting that the effect of Staphylococcus starter cultures on flavour quality is much related to their ability of catabolizing amino acids. With the exception of diacetyl, acetoin and 2-methyl-1-butanol, both cultures formed the same volatiles. Diacetyl and acetoin were not produced...

  4. Not saying I am happy does not mean I am not: cultural influences on responses to positive affect items in the CES-D.

    Science.gov (United States)

    Jang, Yuri; Kwag, Kyung Hwa; Chiriboga, David A

    2010-11-01

    Given the emphasis on modesty and self-effacement in Asian societies, the present study explored differential item responses for 2 positive affect items (5 = Hopeful and 8 = Happy) on a short form of the Center for Epidemiologic Studies-Depression scale. The samples consisted of elderly non-Hispanic Whites (n = 450), Korean Americans (n = 519), and Koreans (n = 2,030). Multiple Indicator Multiple Cause models were estimated to identify the impact of group membership on responses to the positive affect items while controlling for the latent trait of depressive symptoms. The data revealed that Koreans and Korean Americans were less likely than non-Hispanic Whites to endorse the positive affect items. Compared with Korean Americans who were more acculturated to mainstream American culture, those who were less acculturated were less likely to endorse the positive affect items. Our findings support the notion that the way in which people endorse depressive symptoms is substantially influenced by cultural orientation. These findings call into question the common use of simple mean comparisons and a universal cutoff point across diverse cultural groups.

  5. Generation of reactive oxygen species from porous silicon microparticles in cell culture medium.

    Science.gov (United States)

    Low, Suet Peng; Williams, Keryn A; Canham, Leigh T; Voelcker, Nicolas H

    2010-06-01

    Nanostructured (porous) silicon is a promising biodegradable biomaterial, which is being intensively researched as a tissue engineering scaffold and drug-delivery vehicle. Here, we tested the biocompatibility of non-treated and thermally-oxidized porous silicon particles using an indirect cell viability assay. Initial direct cell culture on porous silicon determined that human lens epithelial cells only poorly adhered to non-treated porous silicon. Using an indirect cell culture assay, we found that non-treated microparticles caused complete cell death, indicating that these particles generated a toxic product in cell culture medium. In contrast, thermally-oxidized microparticles did not reduce cell viability significantly. We found evidence for the generation of reactive oxygen species (ROS) by means of the fluorescent probe 2',7'-dichlorofluorescin. Our results suggest that non-treated porous silicon microparticles produced ROS, which interacted with the components of the cell culture medium, leading to the formation of cytotoxic species. Oxidation of porous silicon microparticles not only mitigated, but also abolished the toxic effects.

  6. Yugoslav Naive Art and Popular Culture

    Directory of Open Access Journals (Sweden)

    Meta Kordiš

    2009-12-01

    After the Second World War, the Yugoslav socialist state also strove to equalize and democratize society through art by minimizing the differences between the producers and consumers of art. Such a policy led to the decentralization of culture by forming various cultural and artistic institutions and by holding cultural events and spectacles in the countryside and peripheral areas. Through these various informal ideological mechanisms, the state apparatus exercised its authority in socializing its people in the spirit of Yugoslav socialist self-management and the ideology of brotherhood and unity by joining together the producers and consumers of naive art from various ethnicities, cultures, and social classes. Unfortunately this transformed naive art at its peak of popularity into a decorative and souvenir artifact with a pastoral image and folklore motifs. The encouragement from the authorities on the one hand and the market on the other produced and reproduced simple art forms and narrative contents without a complex iconography, which were consumed uncritically and on a large scale. Consequently, this completely denied the core of naive art and resulted in its final devaluation.

  7. Within- and between-culture variation: individual differences and the cultural logics of honor, face, and dignity cultures.

    Science.gov (United States)

    Leung, Angela K-Y; Cohen, Dov

    2011-03-01

    The CuPS (Culture × Person × Situation) approach attempts to jointly consider culture and individual differences, without treating either as noise and without reducing one to the other. Culture is important because it helps define psychological situations and create meaningful clusters of behavior according to particular logics. Individual differences are important because individuals vary in the extent to which they endorse or reject a culture's ideals. Further, because different cultures are organized by different logics, individual differences mean something different in each. Central to these studies are concepts of honor-related violence and individual worth as being inalienable versus socially conferred. We illustrate our argument with 2 experiments involving participants from honor, face, and dignity cultures. The studies showed that the same "type" of person who was most helpful, honest, and likely to behave with integrity in one culture was the "type" of person least likely to do so in another culture. We discuss how CuPS can provide a rudimentary but integrated approach to understanding both within- and between-culture variation. (c) 2011 APA, all rights reserved

  8. Terminal values and meaning in life among university students with varied levels of altruism in the present period of socio-cultural change

    Directory of Open Access Journals (Sweden)

    Głaz Stanisław

    2012-01-01

    Full Text Available Abstract The author of this paper, interested in the issues of values preference, meaning in life and altruism among university students has attempted to show a relation between them in the present period of clearly noticeable socio-cultural change. The study was conducted in 2009-2010 in Kraków among university students. The age of the respondents ranged from 21 to 25. 200 sets of correctly completed questionnaires were used for the results analysis.

  9. Hematopoietic regulatory factors produced in long-term murine bone marrow cultures and the effect of in vitro irradiation

    International Nuclear Information System (INIS)

    Gualtieri, R.J.; Shadduck, R.K.; Baker, D.G.; Quesenberry, P.J.

    1984-01-01

    The nature of hematopoietic regulatory factors elaborated by the adherent (stromal) cells of long-term murine bone marrow cultures and the effect of in vitro stromal irradiation (XRT) on the production of these factors was investigated. Using an in situ stromal assay it was possible to demonstrate stromal elaboration of at least two colony-stimulating activities, ie, granulocyte/macrophage colony-stimulating activity (G/M-CSA) and megakaryocyte colony-stimulating activity (Meg-CSA). Exposure of the stroma to XRT resulted in dose-dependent elevations of both activities that correlated inversely with total myeloid cell mass. Mixture experiments that combined control and irradiated stroma revealed that the hematopoietically active control stroma could block detection of XRT-related G/M-CSA elevations. Antiserum directed against purified L cell colony-stimulating factor (CSF) reduced granulocyte/macrophage colony formation in the target layer but did not effect the increased Meg-CSA. While a radioimmunoassay for L-cell type CSF was unable to detect significant differences in concentrated media from control and irradiated cultures, bioassays of these media revealed XRT-related G/M-CSA elevations. These results indicate that the G/M-CSA elaborated in these cultures is immunologically distinct from the Meg-CSA produced, and although distinct from L cell CSF, the G/M-CSA is crossreactive with the L cell CSF antiserum. Morphologic, histochemical, and factor VII antigen immunofluorescent studies were performed on the stromal cell population responsible for production of these stimulatory activities. In addition to ''fat'' cells, the stromal cells remaining after XRT were composed of two predominant cell populations. These included a major population of acid phosphatase and nonspecific esterase-positive macrophage-like cells and a minor population of factor VII antigen negative epithelioid cells

  10. Exogenous cellulases of thermophilic micromycetes. Pt. 1. Selection of producers

    Energy Technology Data Exchange (ETDEWEB)

    Kvesitadze, G; Kvachadze, L; Aleksidze, T; Chartishvili, D K

    1986-01-01

    More than 600 micromycetes - representatives of different genera have been investigated for their ability to produce exogenous cellulases. Most of the investigated cultures were found to produce these enzymes, 24 cultures being thermophilic, and 18 thermotolerant. Cellulase or its derivatives proved to be the most favourable carbon source for cellulase secretion. None of the thermophilic cultures studied manifested the ability of exogenous exoglucanase biosynthesis. Using UV-rays as mutagen, a mutant strain A. terreus T-49 has been obtained being characterized by an increased endo-glucanase and cellobiase activity, as compared to the initial strains. The cellulase preparations of thermophilic micromycetes contain one cellulasic component: endo-glucanase, or two: endo-glucanase and cellobiase.

  11. Meme Engineers: Children as Producers of Musical Culture

    Science.gov (United States)

    Barrett, Margaret

    2003-01-01

    This paper examines the ways in which young children construct and negotiate musical meaning as song-makers in a post-modern consumerist musical world. A critical analysis of the presentation of music in two popular Australian children's television programmes (Play School and Hi-5) and the ways in which such media impact upon children's…

  12. Conceptualizing leadership across cultures

    NARCIS (Netherlands)

    Dickson, M.W.; Castaño, N.; Magomaeva, A.; den Hartog, D.N.

    2012-01-01

    In this article, we summarize research on how the meaning of leadership varies systematically across cultures, and describe the conflict in the literature between the quest for universals and the identification of cultural contingencies in leadership theory. We review the literature on the

  13. Perceptions of Emotion from Facial Expressions are Not Culturally Universal: Evidence from a Remote Culture

    Science.gov (United States)

    Gendron, Maria; Roberson, Debi; van der Vyver, Jacoba Marietta; Barrett, Lisa Feldman

    2014-01-01

    It is widely believed that certain emotions are universally recognized in facial expressions. Recent evidence indicates that Western perceptions (e.g., scowls as anger) depend on cues to US emotion concepts embedded in experiments. Since such cues are standard feature in methods used in cross-cultural experiments, we hypothesized that evidence of universality depends on this conceptual context. In our study, participants from the US and the Himba ethnic group sorted images of posed facial expressions into piles by emotion type. Without cues to emotion concepts, Himba participants did not show the presumed “universal” pattern, whereas US participants produced a pattern with presumed universal features. With cues to emotion concepts, participants in both cultures produced sorts that were closer to the presumed “universal” pattern, although substantial cultural variation persisted. Our findings indicate that perceptions of emotion are not universal, but depend on cultural and conceptual contexts. PMID:24708506

  14. Cultural Sensitiveness of School Goals and Students’ Failure in Turkey

    Directory of Open Access Journals (Sweden)

    Ismet Sahin

    2006-06-01

    Full Text Available Education is the means by which society provides for the transmission or advancement of its culture and it is formally done at schools that are the arena of human interaction aimed at producing learning. But some people in that interaction aimed at producing learning cannot achieve as much as the others due to some social or individual factors especially when the society is not homogeneous in terms of culture, language, etc.All cultures do not require the same kinds of knowledge and all may have distinct goals and expectations in education. This study aims at presenting the consensus and conflict in perspectives of students of different ethnic origins on general goals of education and expectations from schools in East and Southeast Turkey. The results will be used to generate a rationale to assume that the failure of students in East and Southeast Turkey where majority of population is ethnically diverse, may be because of the lack of divergent goals and expectations set for school curriculum or that the failure of students is dependent on some other factors except the unique school curriculum unresponsive to cultural or ethnic diversity. For this purpose, the goals of general education (1973, Law number 1739, Item number 2, and school expectations developed by House (1973 were prepared as questionnaire items, piloted, validated and administered to 9373 secondary school students in east and southeast Turkey. The findings of this study were that the students of different ethnic origins value the goals and expectations set for school curriculum in Turkey in significantly different ways.

  15. Cultural Diversity and Multicultural Education

    Science.gov (United States)

    Gay, Geneva

    2013-01-01

    This discussion examines some of the major issues and attributes of culturally responsive teaching. It begins with explaining my views of culturally responsive teaching and how I incorporate cultural responsiveness in my writing to teach readers what it means. These general conceptual frameworks are followed by a discussion of some specific…

  16. Translation Strategy of Chinese Culture-loaded Lexes and the Dissemina-tion of Chinese Culture

    Institute of Scientific and Technical Information of China (English)

    WU Li-li

    2016-01-01

    Lexes are the most important and basic element of a language. Chinese culture-loaded lexes are those words or expres-sions that are greatly rich in Chinese culture. They can reflect the characteristics of Chinese culture and Chinese nation. There-fore, it is of great significance to pay attention to the translation of Chinese culture-loaded lexes as they play a decisive role in disseminating Chinese culture. It can help promote Chinese culture worldwide, improve China’s cultural exchanges and commu-nication with other nations and strengthen China’s status in the world. This paper focuses on the Chinese culture-loaded words and proposes some possible means of translation with the purpose of spreading Chinese culture.

  17. High cell density cultures produced by internal retention: application in continuous ethanol fermentation

    Directory of Open Access Journals (Sweden)

    Berta Carola Pérez

    2004-07-01

    Full Text Available Ethanol has provoked great interest due to its potential as an alternative fuel. Nevertheless, fermentation processes must be developed by increasing the low volumetric productivity achieved in conventional cultures (batch or continuous to make this product become economically competitive. This can be achieved by using techniques leading to high cell concentration and reducing inhibition by the end-product. One of the frequently employed methods involves cell recycling. This work thus developed a membrane reactor incorporating a filtration module with 5 u,m stainless steel tubular units inside a 3L stirred jar fermenter for investigating its application in continuous ethanol production. The effects of cell concentration and transmembrane pressure difference on permeate flux were evaluated for testing the filtration module's performance. The internal cell retention system was operated in Saccharomyces cerevisiae continuous culture derived from sucrose, once fermentation conditions had been selected (30 °C, 1.25 -1.75 vvm, pH 4.5. Filter unit permeability was maintained by applying pulses of air. More than 97% of the grown cells were retained in the fermenter, reaching 51 g/L cell concentration and 8.51 g/L.h average ethanol productivity in culture with internal cell retention; this was twice that obtained in a conventional continuous culture. Key words: Membrane reactor, Saccharomyces cerevisiae, alcoholic fermentation, cell recycling.

  18. Key Process Conditions for Production of C4 Dicarboxylic Acids in Bioreactor Batch Cultures of an Engineered Saccharomyces cerevisiae Strain

    NARCIS (Netherlands)

    Zelle, R.M.; De Hulster, E.; Kloezen, W.; Pronk, J.T.; Van Maris, A.J.A.

    2010-01-01

    A recent effort to improve malic acid production by Saccharomyces cerevisiae by means of metabolic engineering resulted in a strain that produced up to 59 g liter(-1) of malate at a yield of 0.42 mol (mol glucose)(-1) in calcium carbonate-buffered shake flask cultures. With shake flasks, process

  19. Strategic Implications of Culture. Historical Analysis of China's Culture and Implications for United States Policy

    National Research Council Canada - National Science Library

    Crider, Kimberly

    1999-01-01

    .... These differences in interpretation and response are largely rooted in differences in culture, for it is culture that forms the subconscious set of shared meanings that guide group behaviors and perceptions...

  20. A versatile, non genetically modified organism (GMO)-based strategy for controlling low-producer mutants in Bordetella pertussis cultures using antigenic modulation.

    Science.gov (United States)

    Goffin, Philippe; Slock, Thomas; Smessaert, Vincent; De Rop, Philippe; Dehottay, Philippe

    2015-08-01

    The uncontrolled presence of non-producer mutants negatively affects bioprocesses. In Bordetella pertussis cultures, avirulent mutants emerge spontaneously and accumulate. We characterized the dynamics of accumulation using high-throughput growth assays and competition experiments between virulent and avirulent (bvg(-) ) isolates. A fitness advantage of bvg(-) cells was identified as the main driver for bvg(-) accumulation under conditions of high virulence factor production. Conversely, under conditions that reduce their expression (antigenic modulation), bvg(-) takeover could be avoided. A control strategy was derived, which consists in applying modulating conditions whenever virulence factor production is not required. It has a wide range of applications, from routine laboratory operations to vaccine manufacturing, where pertussis toxin yields were increased 1.4-fold by performing early pre-culture steps in modulating conditions. Because it only requires subtle modifications of the culture medium and does not involve genetic modifications, this strategy is applicable to any B. pertussis isolate, and should facilitate regulatory acceptance of process changes for vaccine production. Strategies based on the same concept, could be derived for other industrially relevant micro-organisms. This study illustrates how a sound scientific understanding of physiological principles can be turned into a practical application for the bioprocess industry, in alignment with Quality by Design principles. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Persian Gardens: Meanings, Symbolism, and Design

    Directory of Open Access Journals (Sweden)

    Leila Mahmoudi Farahani

    2016-01-01

    Full Text Available Culture and identity in a society can be represented in the architecture and the meanings intertwined with it. In this sense, the architecture and design are the interface for transferring meaning and identity to the nation and future generations. Persian gardens have been evolved through the history of Persian Empire in regard to the culture and beliefs of the society. This paper aims to investigate the patterns of design and architecture in Persian gardens and the meanings intertwined with their patterns and significant elements such as water and trees. Persian gardens are not only about geometries and shapes; but also manifest different design elements, each representing a specific symbol and its significance among the society. This paper seeks to explore Persian gardens in terms of their geometric structure, irrigation system, network construction and pavilions alongside design qualities such as hierarchy, symmetry, centrality, rhythm and harmony. In the second stage, the paper investigates the fundamental symbols and their philosophy in the creation of Persian gardens and in relation to the architecture and design.

  2. A monument to the player: preserving a landscape of socio-cultural capital in the transitional MMORPG

    Science.gov (United States)

    `Adrir'Scott, Michael

    2012-12-01

    Massively multiplayer online role-playing games (MMORPGs) produce dynamic socio-ludic worlds that nurture both culture and gameplay to shape experiences. Despite the persistent nature of these games, however, the virtual spaces that anchor these worlds may not always be able to exist in perpetuity. Encouraging a community to migrate from one space to another is a challenge now facing some game developers. This paper examines the case of Guild Wars® and its "Hall of Monuments", a feature that bridges the accomplishments of players from the current game to the forthcoming sequel. Two factor analyses describe the perspectives of 105 and 187 self-selected participants. The results reveal four factors affecting attitudes towards the feature, but they do not strongly correlate with existing motivational frameworks, and significant differences were found between different cultures within the game. This informs a discussion about the implications and facilitation of such transitions, investigating themes of capital, value perception and assumptive worlds. It is concluded that the way subcultures produce meaning needs to be considered when attempting to preserve the socio-cultural landscape.

  3. Biotransformations with plant tissue cultures.

    Science.gov (United States)

    Carew, D P; Bainbridge, T

    1976-01-01

    Suspension cultures of Catharanthus roseus, Apocynum cannabinum and Conium maculatum were examined for their capacity to transform aniline, anisole, acetanilide, benzoic acid and coumarin. None of the cultures transformed acetanilide but each produced acetanilide when fed aniline. All three cultures converted benzoic acid to its para-hydroxy derivative. Coumarin was selectively hydroxylated at the 7-position by Catharanthus and Conium and anisole was O-demethylated only by older Catharanthus tissue.

  4. Cultures of choice: towards a sociology of choice as a cultural phenomenon.

    Science.gov (United States)

    Schwarz, Ori

    2017-09-07

    The article explores different ways to conceptualize the relationship between choice and culture. These two notions are often constructed as opposites: while sociologies of modernization (such as Giddens') portray a shift from cultural traditions to culturally disembedded choice, dispositional sociologies (such as Bourdieu's) uncover cultural determination as the hidden truth behind apparent choice. However, choice may be real and cultural simultaneously. Culture moulds choice not only by inculcating dispositions or shaping repertoires of alternatives, but also by offering culturally specific choice practices, ways of choosing embedded in meaning, normativity, and materiality; and by shaping attributions of choice in everyday life. By bringing together insights from rival schools, I portray an outline for a comparative cultural sociology of choice, and demonstrate its purchase while discussing the digitalization of choice; and cultural logics that shape choice attribution in ways opposing neoliberal trends. © London School of Economics and Political Science 2017.

  5. Culture and teams.

    Science.gov (United States)

    Kirkman, Bradley L; Shapiro, Debra L; Lu, Shuye; McGurrin, Daniel P

    2016-04-01

    We first review research on culture effects in teams, illustrating that mean levels of team cultural values have main (i.e. direct) effects, indirect effects (i.e. mediated by intervening variables), and moderating influences on team processes and outcomes. Variance in team cultural values or on country of origin (i.e. nationality diversity) also has main effects on team functioning, and we highlight contextual variables that strengthen or weaken these main effects. We next review research examining the effect of variance in team cultural values on global virtual teams, specifically. Finally, we review research on how cultural values shape employees' receptivity to empowering leadership behavior in teams. We conclude by discussing critical areas for future research. Published by Elsevier Ltd.

  6. Distribution of binder in granules produced by means of twin screw granulation

    DEFF Research Database (Denmark)

    Fonteyne, Margot; Fussell, Andrew Luke; Vercruysse, Jurgen

    2014-01-01

    According to the quality by design principle processes may not remain black-boxes and full process understanding is required. The granule size distribution of granules produced via twin screw granulation is often found to be bimodal. The aim of this study was to gain a better understanding...

  7. Keeping patients safe in healthcare organizations: a structuration theory of safety culture.

    Science.gov (United States)

    Groves, Patricia S; Meisenbach, Rebecca J; Scott-Cawiezell, Jill

    2011-08-01

    This paper presents a discussion of the use of structuration theory to facilitate understanding and improvement of safety culture in healthcare organizations. Patient safety in healthcare organizations is an important problem worldwide. Safety culture has been proposed as a means to keep patients safe. However, lack of appropriate theory limits understanding and improvement of safety culture. The proposed structuration theory of safety culture was based on a critique of available English-language literature, resulting in literature published from 1983 to mid-2009. CINAHL, Communication and Mass Media Complete, ABI/Inform and Google Scholar databases were searched using the following terms: nursing, safety, organizational culture and safety culture. When viewed through the lens of structuration theory, safety culture is a system involving both individual actions and organizational structures. Healthcare organization members, particularly nurses, share these values through communication and enact them in practice, (re)producing an organizational safety culture system that reciprocally constrains and enables the actions of the members in terms of patient safety. This structurational viewpoint illuminates multiple opportunities for safety culture improvement. Nurse leaders should be cognizant of competing value-based culture systems in the organization and attend to nursing agency and all forms of communication when attempting to create or strengthen a safety culture. Applying structuration theory to the concept of safety culture reveals a dynamic system of individual action and organizational structure constraining and enabling safety practice. Nurses are central to the (re)production of this safety culture system. © 2011 Blackwell Publishing Ltd.

  8. Experiencing Global Culture in Vatel

    DEFF Research Database (Denmark)

    Kampf, Constance Elizabeth; Broillet, Alexandra; John, Claudette

    2015-01-01

    To explore the meaning of “global culture” in a professional communication context, this paper explores the “unsettled” global culture of Vatel, a private business school educating students from nearly 50 different countries for the hospitality industry. This paper explores the role of knowledge...... management in understanding global culture, arguing that the notion of “unsettled” cultures demonstrates how ideologies function in global settings and draw on national “settled” cultural resources. In unpacking different aspects of Vatel's culture this paper questions assumptions built into cultural frames...... of reference by offering a global culture frame, drawing on cultural resources from country based “settled” cultural contexts, and reflects on how global cultural contexts can benefit from a knowledge management approach to this dynamic between “settled” cultural contexts and “unsettled” cultural ideologies...

  9. Playing up and playing down cultural identity: Introducing cultural influence and cultural variability.

    Science.gov (United States)

    Ferguson, Gail M; Nguyen, Jacqueline; Iturbide, Maria I

    2017-01-01

    Cultural variability (CV) is introduced as an overlooked dimension of cultural identity development pertaining to emphasizing and de-emphasizing the influence of a single cultural identity (i.e., cultural influence [CI]) on daily interactions and behaviors. The Cultural IDentity Influence Measure (CIDIM) is introduced as a novel measure of CI and CV, and hypothesis-driven validation is conducted in two samples along with exploration of associations between CV and well-being. A multicultural sample of 242 emerging adults participated in a daily diary study (Mage = 19.95 years, SDage = 1.40) by completing up to eight daily online surveys containing the CIDIM, criterion measures (ethnic identity, other group orientation, ethnic identity salience and daily variability in salience, social desirability), and measures of personal and interpersonal well-being. A second validation sample (n = 245) completed a 1-time survey with the CIDIM and a subset of criterion measures. Results using both samples show evidence of CI and CV and demonstrate the validity, reliability, and domain-sensitivity of the CIDIM. Further, CV made unique and positive contributions to predicting interaction quality after accounting for ethnic salience and variability in ethnic salience. An analytic approach utilizing standard deviations produced near-identical results to multilevel modeling and is recommended for parsimony. Ethnic minority and majority individuals make daily adjustments to play up and play down the influence of cultural identity on their social interactions and behaviors, and these adjustments predict interpersonal well-being. Cultural influence and cultural variability contribute to our emerging understanding of cultural identity as dynamic and agentic. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  10. LYOPHILIZATION EFFECT ON PRODUCTIVITY OF BUTANOL-PRODUCING STRAINS

    Directory of Open Access Journals (Sweden)

    O. O. Tigunova

    2016-10-01

    Full Text Available Investigation of lyophilization effect on the productivity of butanol-producing strains was the aim of our research. For this purpose we used butanol-producing strains; technical glycerol; biomass of switchgrass Panicum virgatum L. Lyophilization was performed using a lyophilization-drying. The effect of the protective medium on residual moisture of freezedrying cultures suspensions depending on the concentration of glucose and sucrose was studed. It was shown that the lowest residual moisture was attained by using glucose and sucrose in amount of 10% and if the samples of freeze-drying bacteria had been saved for one month at 4 οC the productivity did not decrease. As temperature preservation was increased the productivity of the cultures was gradually decreased and it was greatly reduced at 30 οC. So the protective medium composition was optimized for lyophilization of butanol-producing strains as follows: sucrose 10.0%; gelatin 10.0%; agar 0.02%. It was shown that the preservation of samples of freeze-drying bacteria for six months at a temperature of 4 οC did not affect the productivity of strains. It was found that cultures could use glycerol as a carbon source for butanol accumulation before lyophilization.

  11. Nigerian Students' Perceptions and Cultural Meaning Construction Regarding Academic Integrity in the Online International Classroom

    Science.gov (United States)

    Szilagyi, Annamaria

    2014-01-01

    By presenting perceptions of Nigerian students enrolled in the online international postgraduate programmes of the University of Liverpool regarding academic integrity, this paper aims to explore Western ideas, such as originality and plagiarism that are extraneous in the students' local cultures. Different historical and cultural circumstances…

  12. The conditions of school learning, youth cultures and the use of ICTs in entering high school: the problem of transmission among young people and teachers

    Directory of Open Access Journals (Sweden)

    Alejandro Villa

    2015-12-01

    Full Text Available Abstract In the present paper that comprises the problems of the relation between young cultures and learning are studied, as well as the transmission problems between young people and teachers, in the first year of Middle School. It is a qualitative research which studied deeply some cases. The research makes a comparison among young students and teachers. We use two techniques of research: the semi structured interview and the discussion group. The general objectives are explore and describe the meanings that the incorporation of mass media of communication gets for the for young identities, as well as the consumption and production of young cultures and the impact which introduces their meanings into the generational transmition process between teachers and young people. From our results, we propose three discussion axes: the massification which involves for young people the consumption of young cultures in the first year of Middle School, does not allow visualize, and it could cover up interpersonal violence relations and inequality among themselves as well as their positions of cultural producers; it does exist for young people a marked dissociation in the classroom: on one side, there are the interactions between the group members and consumption/production of young cultures, and, on the other side, the school culture, and the learning process; the problem of attention/inattention of young people in the learning process in the class, is linked to the transmission process, which is produced between teachers and students in the entry into the Middle School. Keywords: Pedagogy. Youth Cultures. Middle School.

  13. Culture, economics, politics and knowledge as meaning-spaces in Social Occupational Therapy: reflections on the experience of “Ponto de Encontro e Cultura”

    Directory of Open Access Journals (Sweden)

    Denise Dias Barros

    2013-12-01

    Full Text Available This article describes and analyzes the “Ponto de Encontro e Cultura - PEC”, an experience developed by METUIA - University of São Paulo (USP-SP in São Paulo from 2007 to 2011 which linked occupational therapy to areas of cultural production. It was attended by the homeless, occupational therapists and students of occupational therapy. To perform the analysis of the different dimensions of the experience we were guided by the meaning-space notion. We took the space as an organizer to understand a reality in which relationships and actions are being empowered in four different spheres: culture, economics, politics and knowledge. We noted that this practice showed that there was an ongoing collective effort to build what may be called piece. This characterizes a process where the space is a common reference point which brings into play different modes of sociability that are created by the management of common symbols and codes. It was important to recognize and appreciate the plurality of modes of knowledge. Thus, we observed that, from the cultural sphere, it is possible to articulate economics, health, social assistance, politics, and knowledge production.

  14. The Extension Storyteller: Using Stories to Enhance Meaning and Catalyze Change

    Science.gov (United States)

    Franz, Nancy

    2016-01-01

    Many cultures share and pass on norms through storytelling. Extension as a culture also creates and shares stories to pass on history, provide information about Extension work and experiences, and develop the organization. However, Extension as a culture less frequently uses storytelling to enhance meaning and catalyze related change. This article…

  15. Bacterial community profiling of milk samples as a means to understand culture-negative bovine clinical mastitis.

    Science.gov (United States)

    Kuehn, Joanna S; Gorden, Patrick J; Munro, Daniel; Rong, Ruichen; Dong, Qunfeng; Plummer, Paul J; Wang, Chong; Phillips, Gregory J

    2013-01-01

    Inflammation and infection of bovine mammary glands, commonly known as mastitis, imposes significant losses each year in the dairy industry worldwide. While several different bacterial species have been identified as causative agents of mastitis, many clinical mastitis cases remain culture negative, even after enrichment for bacterial growth. To understand the basis for this increasingly common phenomenon, the composition of bacterial communities from milk samples was analyzed using culture independent pyrosequencing of amplicons of 16S ribosomal RNA genes (16S rDNA). Comparisons were made of the microbial community composition of culture negative milk samples from mastitic quarters with that of non-mastitic quarters from the same animals. Genomic DNA from culture-negative clinical and healthy quarter sample pairs was isolated, and amplicon libraries were prepared using indexed primers specific to the V1-V2 region of bacterial 16S rRNA genes and sequenced using the Roche 454 GS FLX with titanium chemistry. Evaluation of the taxonomic composition of these samples revealed significant differences in the microbiota in milk from mastitic and healthy quarters. Statistical analysis identified seven bacterial genera that may be mainly responsible for the observed microbial community differences between mastitic and healthy quarters. Collectively, these results provide evidence that cases of culture negative mastitis can be associated with bacterial species that may be present below culture detection thresholds used here. The application of culture-independent bacterial community profiling represents a powerful approach to understand long-standing questions in animal health and disease.

  16. The type of culture medium and the duration of in vitro culture do not influence birthweight of ART singletons.

    Science.gov (United States)

    De Vos, A; Janssens, R; Van de Velde, H; Haentjens, P; Bonduelle, M; Tournaye, H; Verheyen, G

    2015-01-01

    Does the type of in vitro culture medium or the duration of in vitro culture influence singleton birthweight after IVF/ICSI treatment? In a comparison of two culture media, neither the medium nor the duration of culture (Day 3 versus Day 5 blastocyst transfer) had any effect on mean singleton birthweight. Previous studies indicated that in vitro culture of human embryos may affect birthweight of live born singletons. Both the type of culture medium and the duration of culture may be implicated. However, these studies are small and report conflicting results. A large retrospective analysis was performed including all singleton live births after transferring fresh Day 3 or Day 5 embryos. IVF and ICSI cycles performed between April 2004 and December 2009 at a tertiary care centre were included for analysis. A total of 2098 singleton live births resulting from singleton pregnancies were included for analysis. Two different sequential embryo culture media were concurrently used in an alternating way: Medicult (n = 1388) and Vitrolife (n = 710). Maternal age, maternal and paternal BMI, maternal parity, maternal smoking, main cause of infertility, cycle rank, stimulation protocol, method of fertilization (IVF or ICSI), time in culture and number of embryos transferred were taken into account. Embryo transfers were performed either on Day 3 (n = 1234) or on Day 5 (n = 864). Singleton birthweight was the primary outcome parameter. Gestational age and gender of the newborn were accounted for in the multiple regression analysis. No significant differences in mean singleton birthweight were observed between the two culture media: Medicult 3222 g (±15 SE) and Vitrolife 3251 g (±21 SE) (P = 0.264). The mean singleton birthweight was not different between Day 3 embryo transfers (3219 ± 16 g) and Day 5 blastocyst transfers (3250 ± 19 g; P = 0.209). Multiple regression analysis controlling for potential maternal, paternal, treatment and newborn confounders confirmed the non

  17. Gender, culture and changing attitudes: experiences of HIV in Zimbabwe.

    Science.gov (United States)

    O'Brien, Stephen; Broom, Alex

    2013-01-01

    This paper draws on a series of qualitative interviews with 60 people living in economically poor communities of Harare, the capital of Zimbabwe, to provide new insight into the cultural landscape of HIV. While there has been extensive exploration of gender, sexuality, culture and HIV in Zimbabwe, there is a need to revisit these issues given the country's recent political and economic history. These questions have shaped the meanings that have been created around HIV (i.e., notions of HIV-as-death and as being produced by promiscuity) and the gendered mediation of cultural practices (i.e., forms of sexual expression and treatment uptake). Drawing on the accounts from a group directly affected by HIV, we illustrate the persistence of gendered and spiritualised ideas about 'blame', 'transmission' and 'treatment' and the disproportionate burden that still falls on Zimbabwean women. We conclude with an exploration of how everyday understandings of HIV may be shifting and the ways in which marginality, discrimination and stigma may be being challenged by openness, dialogue and attitude change.

  18. Kinetics of early in vitro development of bovine in vivo- and in vitro-derived zygotes produced and/or cultured in chemically defined or serum-containing media

    DEFF Research Database (Denmark)

    Holm, P; Booth, P J; Callesen, H

    2002-01-01

    The kinetics of the in vitro development of early embryos from bovine zygotes derived in vitro and in vitro were compared, investigating the effect of serum during in vitro maturation and fertilization (IVM-IVF) and in culture. Zygotes were collected from superovulated heifers or produced in vitro...... to the compact morula or blastocyst stages (87% versus 47-54 respectively; P

  19. Consumer Culture, Sustainability and Business Practice: How Companies can Promote the Symbolic Value of Sustainability in Consumption Activities?

    Directory of Open Access Journals (Sweden)

    Verônica Macário de Oliveira

    2016-05-01

    Full Text Available Discussions on sustainability began recently to focus on the consumption patterns of contemporary society as a major causative factors of social and environmental problems. Thus, the aim of this paper is to discuss some opportunities that companies have to influence these changes consumption patterns towards sustainability, taking as a basis the view discussed in studies of Rindova and Ravasi (2008 who consider firms as producers of culture. To this end, we performed a theoretical essay. The results show that companies can influence the formation of specific cultures with the symbolic construction of sustainable practices, contributing to the formation of a culture of sustainable consumption. This occurs from innovation in their ways of working, considering that evoke meanings that products appear to be influenced by strategic choices of producers, such as the concepts and philosophies of design (Ravasi; Rindova, 2008, which includes the development new technologies and practices (Michaelis, 2003 based on the principles of eco-efficiency (Barber, 2008; Clark, 2008, as well as changes in values and discourses that shape the cultures of business, government, media and civil society (Michaelis, 2003, also aligned with the ethical principles and shared environmental responsibility (Tukkeret al, 2008.

  20. [Does work have a meaning? Basis for a positive work culture].

    Science.gov (United States)

    Sapelli, G

    2010-01-01

    From the anthropological point of view work is the expression and fulfilment of the absolute spirit in the finite sense and is, by its essence, cooperation. This obliges us to reverse the question: "does work have a meaning?", to: "does work have a meaning for workers? Does it allow self-fulfilment?" Work has a meaning if the worker's psychophysical integrity is safeguarded, if by working the individual achieves self-fulfilment as a person, if it has a sense of community. Man as an "actor" achieves self-fulfilment through technical and professional competence, thereby overcoming organizational and social conflicts. Today reducing human and social relations to an economic transaction will lead to the destruction of the meaning of life and work. The study of suicide cases in France Telecom shows that a human being inserted in the working environment wants most of all to obtain recognition. He or she will accept praise or even punishment if this is done with transparency, because the person will acknowledge in such decisions a recognition of his/herself. Many enterprises and consequently many personnel managers do not, in their essence, perceive of any meaning of work, but consider only the instrumental element. The workforce cannot be considered as a commodity, work is embodied in human beings. So either people aim at self-fulfilment or fall into anomie and depression, even to the point of the destructive impulse of elimination. The re-humanization of work and the treatment of enterprises (which are "ill" because they no longer possess any meaning of work) is the challenge that awaits us.

  1. Understanding Grief within a Cultural Context

    Science.gov (United States)

    ... the loved ones who are preparing for their loss. Culture and the meaning of death Every culture has ... and mourning practices of a person from another culture. Related Resources Coping With Grief Coping With Change After a Loss f t k e P Coping With Cancer ...

  2. Culture, salience, and psychiatric diagnosis: exploring the concept of cultural congruence & its practical application.

    Science.gov (United States)

    Rashed, Mohammed Abouelleil

    2013-07-16

    Cultural congruence is the idea that to the extent a belief or experience is culturally shared it is not to feature in a diagnostic judgement, irrespective of its resemblance to psychiatric pathology. This rests on the argument that since deviation from norms is central to diagnosis, and since what counts as deviation is relative to context, assessing the degree of fit between mental states and cultural norms is crucial. Various problems beset the cultural congruence construct including impoverished definitions of culture as religious, national or ethnic group and of congruence as validation by that group. This article attempts to address these shortcomings to arrive at a cogent construct. The article distinguishes symbolic from phenomenological conceptions of culture, the latter expanded upon through two sources: Husserl's phenomenological analysis of background intentionality and neuropsychological literature on salience. It is argued that culture is not limited to symbolic presuppositions and shapes subjects' experiential dispositions. This conception is deployed to re-examine the meaning of (in)congruence. The main argument is that a significant, since foundational, deviation from culture is not from a value or belief but from culturally-instilled experiential dispositions, in what is salient to an individual in a particular context. Applying the concept of cultural congruence must not be limited to assessing violations of the symbolic order and must consider alignment with or deviations from culturally-instilled experiential dispositions. By virtue of being foundational to a shared experience of the world, such dispositions are more accurate indicators of potential vulnerability. Notwithstanding problems of access and expertise, clinical practice should aim to accommodate this richer meaning of cultural congruence.

  3. Reading Difference: Picture Book Retellings as Contexts for Exploring Personal Meanings of Race and Culture

    Science.gov (United States)

    Lysaker, Judith; Sedberry, Tiffany

    2015-01-01

    In racially and culturally homogeneous school settings, opportunities for children to interact with those who are unlike themselves are not always available. Picture book retellings provide contexts within which students are exposed to racial and cultural differences by allowing them to engage in vicarious events with people they might not…

  4. Study of the defect structures produced by heavy ions in dielectrics by means of small angle scattering

    International Nuclear Information System (INIS)

    Albrecht, D.J.

    1983-11-01

    The aim of the present thesis was to study the defects in dielectrics produced by fast ions. For this the small angle scattering was proved as suitable method. By the production by means of the ion beam of an accelerator the defects possess a pronounced preferential direction. In scattering experiments this system of scattering centers is distinguished by its unusually strong dependence of the sample orientation according to the primary beam. This property was studied, described, and illustrated by examples, and it could by shown that from this additional informations about the scattering defects can be derived. For the first time nuclear tracks were detected by means of small angle neutron scattering. It could be shown that here the same results are obtained as by small angle X-ray scattering. The measured intensity distributions could be assigned to a mathematical model description which gives form, width, and density of the tracks in the position space. On the base of this cylinder model computer codes were established which simulate the scattering experiment regarding the main influences and calculate the expected theoretical intensity distribution. The agreement between calculated and measured scattering distributions proves the validity of the model. The parameters determined by this model, maximal change of density in the track, defect length, radial dilatation, distance of the narrowings and there depth were determined. For the main quantities, radial dilatation and maximal change of densities a simple description of the energy dependence could be given via the energy loss. (orig./HSI) [de

  5. Stakeholder Understandings of Wildfire Mitigation: A Case of Shared and Contested Meanings

    Science.gov (United States)

    Champ, Joseph G.; Brooks, Jeffrey J.; Williams, Daniel R.

    2012-10-01

    This article identifies and compares meanings of wildfire risk mitigation for stakeholders in the Front Range of Colorado, USA. We examine the case of a collaborative partnership sponsored by government agencies and directed to decrease hazardous fuels in interface areas. Data were collected by way of key informant interviews and focus groups. The analysis is guided by the Circuit of Culture model in communication research. We found both shared and differing meanings between members of this partnership (the "producers") and other stakeholders not formally in the partnership (the "consumers"). We conclude that those promoting the partnership's project to mitigate risk are primarily aligned with a discourse of scientific management. Stakeholders outside the partnership follow a discourse of community. We argue that failure to recognize and account for differences in the way risk mitigation is framed and related power dynamics could hamper the communicational efforts of the collaborative partnership and impact goals for fuels reduction. We recommend ways that both groups can capitalize on shared meanings and how agency managers and decision makers can build better working relationships with interface communities and other external stakeholders.

  6. Stakeholder understandings of wildfire mitigation: a case of shared and contested meanings.

    Science.gov (United States)

    Champ, Joseph G; Brooks, Jeffrey J; Williams, Daniel R

    2012-10-01

    This article identifies and compares meanings of wildfire risk mitigation for stakeholders in the Front Range of Colorado, USA. We examine the case of a collaborative partnership sponsored by government agencies and directed to decrease hazardous fuels in interface areas. Data were collected by way of key informant interviews and focus groups. The analysis is guided by the Circuit of Culture model in communication research. We found both shared and differing meanings between members of this partnership (the "producers") and other stakeholders not formally in the partnership (the "consumers"). We conclude that those promoting the partnership's project to mitigate risk are primarily aligned with a discourse of scientific management. Stakeholders outside the partnership follow a discourse of community. We argue that failure to recognize and account for differences in the way risk mitigation is framed and related power dynamics could hamper the communicational efforts of the collaborative partnership and impact goals for fuels reduction. We recommend ways that both groups can capitalize on shared meanings and how agency managers and decision makers can build better working relationships with interface communities and other external stakeholders.

  7. Language, Culture, and Cognition in Cross-cultural Communication

    OpenAIRE

    Nardon, Luciara; Steers, Richard; Stone, Christian

    2012-01-01

    It is well documented that communication styles and patterns vary across cultures. However, less is known about the process underlying these differences. Understanding why communication patterns vary is just as important as understanding how they vary because communication is by nature a dynamic and interactive process. Despite the importance of the transmission of meaning for successful communication, and the role that cognition plays in the assignment of meaning, little has been done to dra...

  8. Conversation at the Border Between Organizational Culture Theory and Institutional Theory

    DEFF Research Database (Denmark)

    Hatch, Mary Jo; Zilber, Tammar

    2012-01-01

    This paper reflects our conversation at the border - a dividing line but also a potential meeting place - of organizational culture theory and institutional theory. First, we discuss the border between institutional theory and organizational culture theory by exploring two notions central to both...... - taken for grantedness and meanings. We ask what is taken for granted about institutions and organizational culture and how institutions and organizational cultures materialize? Our conversation reveals that although the notion of the taken for granted is important to institutional theory...... and organizational culture theory, what this means and implies is quite different for each. We also found that even though institutions and cultures involve meaning and evolve through meaning making, the two are understood and hence explored methodologically in quite different ways. So what seemed to be similar...

  9. Estimation by limiting dilution analysis of human IL 2-secreting T cells: detection of IL 2 produced by single lymphokine-secreting T cells

    International Nuclear Information System (INIS)

    Vie, H.; Miller, R.A.

    1986-01-01

    We present here a culture method for the estimation, in human blood, of the number of lymphocytes that can respond to mitogen by producing interleukin 2 (IL 2). T cells are cultured at limiting dilutions with PHA or Con A in the presence of Epstein Barr virus-transformed human lymphoblastoid cells (EB-LCL), and supernatants are tested 3 days later for IL 2 content by a cell proliferation assay. The distribution of negative wells follows the expected Poisson single-hit relationship, suggesting that the assay is sensitive to single cells of a single limiting cell type. On average, 16.3% of peripheral blood mononuclear cells can produce IL 2 in such clonal cultures (mean of 12 determinations; SD = 5.6%). Surprisingly, irradiation (up to 2000 rad) of the titrated responder cell population diminishes the estimated frequencies by less than 50%. The ability to detect IL 2 levels in cultures containing only a single, nonproliferating T lymphocyte allows us to estimate the amount of IL 2 generated by an individual effector cell during a 3-day culture interval after mitogen stimulation. The average responding, irradiated T cell generates 0.92 pg of IL 2 (median) within 3 days. The method presented provides a straightforward way to provide independent estimates of responding cell number and of lymphokine production per cell in a variety of clinical situations

  10. CHALLENGES IN CROSS CULTURAL ADVERTISING

    Directory of Open Access Journals (Sweden)

    Yuni Retnowati

    2016-04-01

    Full Text Available At first, marketing practitioners and academics consider standardized approaches to marketing and advertising strategies in globalization, and then some studies proved that the standardization of advertising across culture is not valid. Therefore, cross cultural advertising takes local culture into account when conveying messages in advertisements. Cross cultural understanding is very important in order to produce successful localized advertising that would reflect the cultural values and norms of intended audience. Challenge in cross cultural advertising is the problem of communicating to people of diverse cultural background. Cross cultural solutions are applied in areas such as language, communication style, images and cultural values. Cross cultural advertising is simply about using common sense and analyzing how the different elements of an advertising campaign are impacted by culture and modifying them to best speak to the target audience. Other challenges are determining between standardization and adaptation of cultural values content of advertising when facing different people from diverse cultures. In academic side, the challenge is preparing students to design advertisements that communicate effectively to diverse cultures.

  11. The consequences of "Culture's consequences"

    DEFF Research Database (Denmark)

    Knudsen, Fabienne; Froholdt, Lisa Loloma

    2009-01-01

      In this article, it is claimed that research on cross-cultural crews is dominated by one specific understanding of the concept of culture, which is static, evenly distributed and context-independent. Such a conception of culture may bring some basic order while facing an unknown culture...... review of the theory of Geert Hofstede, the most renowned representative of this theoretical approach. The practical consequences of using such a concept of culture is then analysed by means of a critical review of an article applying Hofstede to cross-cultural crews in seafaring. Finally, alternative...... views on culture are presented. The aim of the article is, rather than to promote any specific theory, to reflect about diverse perspectives of cultural sense-making in cross-cultural encounters. Udgivelsesdato: Oktober...

  12. 7 CFR 1205.319 - Cotton-producing region.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Cotton-producing region. 1205.319 Section 1205.319... Cotton Research and Promotion Order Definitions § 1205.319 Cotton-producing region. Cotton-producing region means each of the following groups of cotton-producing States: (a) Southeast Region: Alabama...

  13. Call for papers-special issue on Europe’s culture(s): Negotiating cultural meanings, values, and identities in the European context

    OpenAIRE

    Benet-Martinez, V; Mesquita, Batja; Uskul, A; Gobel, M

    2016-01-01

    Europe has recently been facing several unprecedented and deeply significant challenges to its identity: From the Charlie Hebdo murders and Paris shootings in France to the PEGIDA discussions in Germany, from the possibility of Grexit to the UK discussions to leave the EU, and from pro-independence movements within Spain and the UK to other countries working on reunification (Cyprus), and perhaps most importantly the current refugee crisis. In all these cases, the meaning(s) of European cultu...

  14. Using individualism and collectivism to compare cultures--a critique of the validity and measurement of the constructs: comment on Oyserman et al. (2002).

    Science.gov (United States)

    Fiske, Alan Page

    2002-01-01

    Analyzing national and ethnic differences in individualism and collectivism, D. Oyserman, H. M. Coon, and M. Kemmelmeier (2002) showed that small differences in scales or samples produce markedly divergent results, challenging the validity of these constructs. The author examines the following limitations of research on individualism and collectivism: It treats nations as cultures and culture as a continuous quantitative variable; conflates all kinds of social relations and distinct types of autonomy; ignores contextual specificity in norms and values; measures culture as the personal preferences and behavior reports of individuals; rarely establishes the external validity of the measures used; assumes cultural invariance in the meaning of self-reports and anchoring and interpretation of scales; and reduces culture to explicit, abstract verbal knowledge.

  15. Lip dubbing on YouTube: Participatory culture and cultural globalization

    Directory of Open Access Journals (Sweden)

    Mark C. Lashley

    2012-09-01

    Full Text Available In the phenomenon of lip dubbing online, music fans throughout the world mime along with their favorite (usually Western pop songs, and distribute videos of the performances to web video sites like YouTube. Two popular examples of the form are examined: China's Back Dorm Boys, and Moymoy Palaboy, from the Philippines. The dozens of videos produced by each group speak to issues of cultural imperialism and globalization, as well as broader concerns about participatory culture within the YouTube space.

  16. The philosophical and educational potential ecopsychology as means of forming ecological culture of personality

    Directory of Open Access Journals (Sweden)

    O. V. Kisyel

    2014-09-01

    Full Text Available The current ecological crisis is largely caused by the dominance of anthropocentric environmental awareness. To overcome it and move humanity to a model of sustainable social and environmental development required the establishment of environmental consciousness ecocentric type, which is the aim of environmental education, namely, the formation of personality type ecocentric environmental awareness. The purpose of the article is to study the philosophical and educational potential environmental psychology as a means of transformation of personal values and attitudes toward nature. Analysis of the concepts of subjective attitude to nature, subjective perception of the natural world, environmental knowledge, empaurment-method pedagogy. Education for Sustainable Development aims to develop such knowledge, skills and values that will enable people to make individual and collective decisions of local and global nature to improve the quality of life without endangering future generations. The basic methodological principle of environmental psyhopedahohiky is in strict accordance with the environmental education teaching psychological process of environmental awareness. Interaction with nature is a great psychological and pedagogical potential that should be used in the environmental education that will allow him to become a factor in the overall development and personality development. So effectively targeted formation ekospozhyvchoyi culture of all populations requires training of qualified specialists in the field of eco-educational activities and environmental education and its philosophical and educational foundation.

  17. Current state of purification, isolation and analysis of bacteriocins produced by lactic acid bacteria.

    Science.gov (United States)

    Kaškonienė, Vilma; Stankevičius, Mantas; Bimbiraitė-Survilienė, Kristina; Naujokaitytė, Gintarė; Šernienė, Loreta; Mulkytė, Kristina; Malakauskas, Mindaugas; Maruška, Audrius

    2017-02-01

    The scientific interest for the search of natural means of microbial inhibitors has not faded for several years. A search of natural antibiotics, so-called bacteriocins which are produced by lactic acid bacteria (LAB), gains a huge attention of the scientists in the last century, in order to reduce the usage of synthetic food additives. Pure bacteriocins with wide spectra of antibacterial activity are promising among the natural biopreservatives. The usage of bacteriocin(s) producing LAB as starter culture for the fermentation of some food products, in order to increase their shelf-life, when synthetic preservatives are not allowable, is also possible. There are a lot of studies focusing on the isolation of new bacteriocins from traditional fermented food, dairy products and other foods or sometimes even from unusual non-food matrices. Bacteriocins producing bacteria have been isolated from different sources with the different antibacterial activity against food-borne microorganisms. This review covers the classification of bacteriocins, diversity of sources of bacteriocin(s) producing LAB, antibacterial spectra of isolated bacteriocins and analytical methods for the bacteriocin purification and analysis within the last 15 years.

  18. CULTURAL ISSUES IN ECONOMICS

    OpenAIRE

    Maciej Meyer

    2012-01-01

    This article has been written with the purpose of attracting attention to the cultural issues, or rather lack of them, in economics. This topic has not been taken frequently into theoretical considerations due to some difficulties, although its practical implications are of great importance. The meaning of institutions which are a part of cultures has been given more coverage in the literature. The following hypothesis is proposed: culture is an important but underestimated component of the e...

  19. 7 CFR 1033.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... Handling Definitions § 1033.13 Producer milk. Producer milk means the skim milk (or the skim equivalent of components of skim milk), including nonfat components, and butterfat in milk of a producer that is: (a... percentages in paragraph (d)(4) of this section may be increased or decreased by the market administrator if...

  20. 7 CFR 1001.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... Handling Definitions § 1001.13 Producer milk. Producer milk means the skim milk (or the skim equivalent of components of skim milk) and butterfat contained in milk of a producer that is: (a) Received by the operator... be increased or decreased by the Market Administrator if the Market Administrator finds that such...

  1. 7 CFR 1126.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... Handling Definitions § 1126.13 Producer milk. Producer milk means the skim milk (or the skim equivalent of components of skim milk), including nonfat components, and butterfat contained in milk of a producer that is...) of this section may be increased or decreased by the market administrator if there is a finding that...

  2. Divide and conquer: intermediate levels of population fragmentation maximize cultural accumulation.

    Science.gov (United States)

    Derex, Maxime; Perreault, Charles; Boyd, Robert

    2018-04-05

    Identifying the determinants of cumulative cultural evolution is a key issue in the interdisciplinary field of cultural evolution. A widely held view is that large and well-connected social networks facilitate cumulative cultural evolution because they promote the spread of useful cultural traits and prevent the loss of cultural knowledge through factors such as drift. This view stems from models that focus on the transmission of cultural information, without considering how new cultural traits actually arise. In this paper, we review the literature from various fields that suggest that, under some circumstances, increased connectedness can decrease cultural diversity and reduce innovation rates. Incorporating this idea into an agent-based model, we explore the effect of population fragmentation on cumulative culture and show that, for a given population size, there exists an intermediate level of population fragmentation that maximizes the rate of cumulative cultural evolution. This result is explained by the fact that fully connected, non-fragmented populations are able to maintain complex cultural traits but produce insufficient variation and so lack the cultural diversity required to produce highly complex cultural traits. Conversely, highly fragmented populations produce a variety of cultural traits but cannot maintain complex ones. In populations with intermediate levels of fragmentation, cultural loss and cultural diversity are balanced in a way that maximizes cultural complexity. Our results suggest that population structure needs to be taken into account when investigating the relationship between demography and cumulative culture.This article is part of the theme issue 'Bridging cultural gaps: interdisciplinary studies in human cultural evolution'. © 2018 The Author(s).

  3. Postcolonial constructions of HIV/AIDS: meaning, culture, and structure.

    Science.gov (United States)

    Sastry, Shaunak; Dutta, Mohan J

    2011-01-01

    As a field of inquiry, postcolonial health communication seeks to apprehend processes implicated in the construction of "primitive" versus "modern" with respect to issues of health. In the case of HIV/AIDS, the sociocultural representations of the disease have a profound impact on how the disease is configured medically and symbolically in dominant cultural imagination. Postcolonial constructions of disease are mobilized around the political and economic interests of the dominant power structures in global spaces. In this article, a thematic analysis of the constructions of HIV/AIDS in India in the mainstream U.S. news media was conducted. A corpus of news articles from the Lexis-Nexis database was created with the keywords "HIV," "AIDS," and "India." Three themes emerged from the study: (a) India as a site of biomedical control; (b) the economic logics of HIV/AIDS; and (c) AIDS, development, and the "Third World." Copyright © Taylor & Francis Group, LLC

  4. Semantic Songket Image Search with Cultural Computing of Symbolic Meaning Extraction and Analytical Aggregation of Color and Shape Features

    Directory of Open Access Journals (Sweden)

    Desi Amirullah

    2015-06-01

    Full Text Available The term "Songket" comes from the Malay word "Sungkit", which means "to hook" or "to gouge". Every motifs names and variations was derived from plants and animals as source of inspiration to create many patterns of songket. Each of songket patterns have a philosophy in form of rhyme that refers to the nature of the sources of songket patterns and that philosophy reflects to the beliefs and values of Malay culture. In this research, we propose a system to facilitate an understanding of songket and the philosophy as a way to conserve Songket culture. We propose a system which is able to collect information in image songket motif variations based on feature extraction methods. On each image songket motif variations, we extracted philosophy of rhyme into impressions, and extracting color features of songket images using a histogram 3D-Color Vector quantization (3D-CVQ, shape feature extraction songket image using HU Moment invariants. Then, we created an image search based on impressions, and impressions search based on image. We use techniques of search based on color, shape and aggregation (combination of colors and shapes. The experiment using impression as query : 1 Result based on color, the average value of true 7.3, total score 41.9, 2 Result based on shape, the average value of true 3, total score 16.4, 3 Result based on aggregation, the average value of true 3, total score 17.4. While based using Image Query : 1 Result based on color, the average precision 95%, 2 Result based on shape, average precision 43.3%, 3 Based aggregation, the average precision 73.3%. From our experiments, it can be concluded that the best search system using query impression and query image is based on the color. Keyword : Image Search, Philosophy, impression, Songket, cultural computing, Feature Extraction, Analytical aggregation.

  5. The Impact of National Culture on the Organizational Culture

    Directory of Open Access Journals (Sweden)

    ALICIA FLORENCIA URTEAGA

    2010-03-01

    Full Text Available The relationships between national culture and organizational culture were analysed in 16 Argentinean companies. Sample was integrated by 429 employees (292 male, and 184 female, mean age 35 years old. Subjects completed, in their own work places, a socio-demographic questionnaire,a scale of national culture, and a scale of organizational practices. Results indicated that organizations adopt practices that, on average, reflect the cultural values of their country of origin. Multiple regression analysis showedthat Argentinean national companies are characterized by practices closely associated with collectivism, high power distance, and high uncertainty avoidance. In contrast, Argentinean privatized companies adopted practices most linked to individualism, low power distance, low uncertainty avoidance,low paternalism, and low fatalism. The results are discussed in light of its strengths and weaknesses, and a new agenda for future research is suggested.

  6. Language as a Facilitator of Cultural Connection

    Science.gov (United States)

    Gonzalez, Miigis B.; Aronson, Benjamin D.; Kellar, Sidnee; Walls, Melissa L.; Greenfield, Brenna L.

    2018-01-01

    Understanding culture as a means of preventing or treating health concerns is growing in popularity among social behavioral health scientists. Language is one component of culture and therefore may be a means to improve health among Indigenous populations. This study explores language as a unique aspect of culture through its relationship to other demographic and cultural variables. Participants (n = 218) were adults who self-identified as American Indian, had a type 2 diabetes diagnosis, and were drawn from two Ojibwe communities using health clinic records. We used chi-squared tests to compare language proficiency by demographic groups and ANOVA tests to examine relationships between language and culture. A higher proportion of those living on reservation lands could use the Ojibwe language, and fluent speakers were most notably sixty-five years of age and older. Regarding culture, those with greater participation and value belief in cultural activities reported greater language proficiency. PMID:29782622

  7. Recombinant Protein Production and Insect Cell Culture and Process

    Science.gov (United States)

    Spaulding, Glenn F. (Inventor); Goodwin, Thomas J. (Inventor); OConnor, Kim C. (Inventor); Francis, Karen M. (Inventor); Andrews, Angela D. (Inventor); Prewett, Tracey L. (Inventor)

    1997-01-01

    A process has been developed for recombinant production of selected polypeptides using transformed insect cells cultured in a horizontally rotating culture vessel modulated to create low shear conditions. A metabolically transformed insect cell line is produced using the culture procedure regardless of genetic transformation. The recombinant polypeptide can be produced by an alternative process using virtually infected or stably transformed insect cells containing a gene encoding the described polypeptide. The insect cells can also be a host for viral production.

  8. Librarians and Party Girls: Cultural Studies and the Meaning of the Librarian.

    Science.gov (United States)

    Radford, Marie L.; Radford, Gary P.

    2003-01-01

    Responds to Wayne Wiegand's article that criticizes library and information science by suggesting a cultural studies approach to the field. Applies the work of Stuart Hall to a media stereotype of the female librarian based on the film "Party Girl" to allow new interpretations of media images of the profession. (Author/LRW)

  9. Cultural Diplomacy in Europe.

    Science.gov (United States)

    Haigh, Anthony

    The evolution of European government activities in the sphere of international cultural relations is examined. Section 1 describes the period between World War I and World War II when European governments tried to enhance their prestige and policies by means of cultural propaganda. Section 2 analyzes the period during World War II when the…

  10. Sound as Popular Culture

    DEFF Research Database (Denmark)

    The wide-ranging texts in this book take as their premise the idea that sound is a subject through which popular culture can be analyzed in an innovative way. From an infant’s gurgles over a baby monitor to the roar of the crowd in a stadium to the sub-bass frequencies produced by sound systems...... in the disco era, sound—not necessarily aestheticized as music—is inextricably part of the many domains of popular culture. Expanding the view taken by many scholars of cultural studies, the contributors consider cultural practices concerning sound not merely as semiotic or signifying processes but as material......, physical, perceptual, and sensory processes that integrate a multitude of cultural traditions and forms of knowledge. The chapters discuss conceptual issues as well as terminologies and research methods; analyze historical and contemporary case studies of listening in various sound cultures; and consider...

  11. Critical Consideration on TV Culture

    Institute of Scientific and Technical Information of China (English)

    涂艳

    2015-01-01

    Since TV culture has been the mainstream,TV,as an important cultural media,have spread its influence widely and rapidly so as to alter people’s ideology and life style.The emergency of TV has brought about convenience and happiness and created popularity,but due to its vulgarity,it has inevitably become one of piece of the mainstream culture and a means of social control.

  12. 7 CFR 1006.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... Handling Definitions § 1006.13 Producer milk. Producer milk means the skim milk (or the skim equivalent of components of skim milk) and butterfat contained in milk of a producer that is: (a) Received by the operator... (d) (1) through (3) of this section may be increased or decreased by the market administrator if the...

  13. The outcome of infected total knee arthroplasty: culture-positive versus culture-negative.

    Science.gov (United States)

    Kim, Young-Hoo; Park, Jang-Won; Kim, Jun-Shik; Kim, Dong-Jin

    2015-10-01

    We studied the outcome in culture-positive and culture-negative infected total knee arthroplasty (TKA). We retrospectively reviewed 140 patients with culture-positive and 102 patients with culture-negative infected TKAs. We determined the infection control rate and clinical outcome after repeated debridement, and repeated 2-stage TKA in the culture-positive and culture-negative groups. The mean follow-up was 9.3 years (range 5-14 years) in the culture-positive group and 10.6 years (5-22) in the culture-negative group. The overall infection control rate was 56 % in both groups after the first treatment. The overall infection control rate was 90 % in the culture-positive group and 95 % in the culture-negative group. A functional knee was obtained in 90 % in the culture-positive group and 95 % in the culture-negative group. The data suggest that treatment according to the types of infection in both culture-positive and culture-negative groups after TKA controlled infection and maintained functional TKA with a firm level of fixation for most patients. Repeated debridement and repeated two-stage exchange TKA further improved infection control rates after the initial treatment and increased the likelihood of maintaining a functional TKA.

  14. The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass.

    Science.gov (United States)

    Maki, Miranda; Leung, Kam Tin; Qin, Wensheng

    2009-07-29

    Lignocellulosic biomass is a renewable and abundant resource with great potential for bioconversion to value-added bioproducts. However, the biorefining process remains economically unfeasible due to a lack of biocatalysts that can overcome costly hurdles such as cooling from high temperature, pumping of oxygen/stirring, and, neutralization from acidic or basic pH. The extreme environmental resistance of bacteria permits screening and isolation of novel cellulases to help overcome these challenges. Rapid, efficient cellulase screening techniques, using cellulase assays and metagenomic libraries, are a must. Rare cellulases with activities on soluble and crystalline cellulose have been isolated from strains of Paenibacillus and Bacillus and shown to have high thermostability and/or activity over a wide pH spectrum. While novel cellulases from strains like Cellulomonas flavigena and Terendinibacter turnerae, produce multifunctional cellulases with broader substrate utilization. These enzymes offer a framework for enhancement of cellulases including: specific activity, thermalstability, or end-product inhibition. In addition, anaerobic bacteria like the clostridia offer potential due to species capable of producing compound multienzyme complexes called cellulosomes. Cellulosomes provide synergy and close proximity of enzymes to substrate, increasing activity towards crystalline cellulose. This has lead to the construction of designer cellulosomes enhanced for specific substrate activity. Furthermore, cellulosome-producing Clostridium thermocellum and its ability to ferment sugars to ethanol; its amenability to co-culture and, recent advances in genetic engineering, offer a promising future in biofuels. The exploitation of bacteria in the search for improved enzymes or strategies provides a means to upgrade feasibility for lignocellulosic biomass conversion, ultimately providing means to a 'greener' technology.

  15. [Safety culture: definition, models and design].

    Science.gov (United States)

    Pfaff, Holger; Hammer, Antje; Ernstmann, Nicole; Kowalski, Christoph; Ommen, Oliver

    2009-01-01

    Safety culture is a multi-dimensional phenomenon. Safety culture of a healthcare organization is high if it has a common stock in knowledge, values and symbols in regard to patients' safety. The article intends to define safety culture in the first step and, in the second step, demonstrate the effects of safety culture. We present the model of safety behaviour and show how safety culture can affect behaviour and produce safe behaviour. In the third step we will look at the causes of safety culture and present the safety-culture-model. The main hypothesis of this model is that the safety culture of a healthcare organization strongly depends on its communication culture and its social capital. Finally, we will investigate how the safety culture of a healthcare organization can be improved. Based on the safety culture model six measures to improve safety culture will be presented.

  16. Recognizing Cultural Differences on Food

    OpenAIRE

    Anacleto, Junia c

    2013-01-01

    Cultural differences play a very important role in matching ICT in- teraction to the expectations of users from different national and cultural back- grounds. But to date, there has been few research as to the extent of such differ- ences, and how to produce software that takes into account these differences. Considering the third wave of HCI research on context, involving the intangible aspects of the interaction with users and ICT solutions, like culture, we are studying these issues using ...

  17. Cross-cultural barriers to health care.

    Science.gov (United States)

    Vidaeff, Alex C; Kerrigan, Anthony J; Monga, Manju

    2015-01-01

    Culturally sensitive health care represents a real ethical and practical need in a Western healthcare system increasingly serving a multiethnic society. This review focuses on cross-cultural barriers to health care and incongruent aspects from a cultural perspective in the provision of health care. To overcome difficulties in culturally dissimilar interactions and eventually remove cross-cultural barriers to health care, a culturally sensitive physician considers his or her own identity, values, and beliefs; recognizes the similarities and differences among cultures; understands what those similarities and differences mean; and is able to bridge the differences to accomplish clear and effective communication.

  18. Screening concepts for the isolation of biosurfactant producing microorganisms.

    Science.gov (United States)

    Walter, Vanessa; Syldatk, Christoph; Hausmann, Rudolf

    2010-01-01

    This chapter gives an overview of current methods for the isolation of biosurfactant producing microbes. The common screening methods for biosurfactants are presented. Sampling and isolation of bacteria are the basis for screening of biosurfactant producing microbes. Hydrocarbon-contaminated sites are the most promising for the isolation of biosurfactant producing microbes, but many strains have also been isolated from undisturbed sites. In subsequent steps the isolates have to be characterized in order to identify the strains which are interesting for a further investigation. Several techniques have been developed for identifying biosurfactant producing strains. Most of them are directly based on the surface or interfacial activity of the culture supernatant. Apart from that, some screening methods explore the hydrophobicity of the cell surface. This trait also gives an indication on biosurfactant production. In recent years automation and miniaturization have led to the development of high throughput methods for screening. High throughput screening (HTS) for analyzing large amounts of potential candidates or whole culture collections is reflected in the end. However, no new principals have been introduced by HTS methods.

  19. 7 CFR 1131.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... Handling Definitions § 1131.13 Producer milk. Producer milk means the skim milk (or the skim equivalent of components of skim milk) and butterfat in milk of a producer that is: (a) Received by the operator of a pool... percentage in paragraph (d)(2) of this section may be increased or decreased by the market administrator if...

  20. Comprehension of Disaster Pictorials across Cultures

    Science.gov (United States)

    Blees, Gerda J.; Mak, Willem M.

    2012-01-01

    In different countries, the use of pictorial information symbols to convey warnings and instructions is becoming more common. An important reason for this is that people from a variety of cultures can understand graphical symbols. However, symbols developed in one culture may not have the same meaning for people from other cultures. This study…

  1. CULTURAL ENTREPRENEURSHIP STRATEGIES IN IRAN

    Directory of Open Access Journals (Sweden)

    Fateme Tohidy Ardahaey

    2010-11-01

    Full Text Available This article initially deals with the concepts of entrepreneurship, cultural entrepreneurship and its importance in the field of culture. Then, identifying the factors of cultural entrepreneurshipstrategies, it offers some models for assessing cultural entrepreneurship strategies and benchmarking it. Afterwards, it tries to reform and standardize the presented model through questioning experts and elite of the profession. It is concluded that there is a significant difference between the mean ranks of the implementation factors of the entrepreneurship strategy which is detailed through the work.

  2. Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA

    OpenAIRE

    Vergara,Mauricio; Becerra,Silvana; Díaz-Barrera,Alvaro; Berrios,Julio; Altamirano,Claudia

    2012-01-01

    We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamster ovary cells in batch and semi-perfusion cultures. In batch cultures using glucose as a carbon source, decreasing the temperature caused a reduction in cell growth and an increase in specific pro...

  3. Educational Spaces of Cultural Capitalism: The Concept of Consumer Culture as a New Framework for Contemporary Educational Research

    Science.gov (United States)

    Knobloch, Phillip D. Th.

    2016-01-01

    This article introduces a specific concept of consumer culture into the international and European discussion about new concepts and categories in comparative education. Basic meanings of consumer culture are presented in reference to consumer research, consumer culture theory, and a revisited concept of world polity. In addition to general…

  4. Meanings of Savagery : Terror, Religion and the Islamic State

    NARCIS (Netherlands)

    Nanninga, Pieter; Lewis, James R.

    2017-01-01

    Explanations of the Islamic State’s violence have often focused on its instrumental aspects and perceived it as a means to spread terror and chaos. This chapter argues that the group’s violent actions are not merely a means towards certain ends, but also symbolic actions that embody cultural

  5. Critical Cultural Awareness: Contributions to a Globalizing Psychology

    Science.gov (United States)

    Christopher, John Chambers; Wendt, Dennis C.; Marecek, Jeanne; Goodman, David M.

    2015-01-01

    The number of psychologists whose work crosses cultural boundaries is increasing. Without a critical awareness of their own cultural grounding, they risk imposing the assumptions, concepts, practices, and values of U.S.-centered psychology on societies where they do not fit, as a brief example from the 2004 Indian Ocean tsunami shows. Hermeneutic thinkers offer theoretical resources for gaining cultural awareness. Culture, in the hermeneutic view, is the constellation of meanings that constitutes a way of life. Such cultural meanings – especially in the form of folk psychologies and moral visions – inevitably shape every psychology, including U.S. psychology. The insights of hermeneutics, as well as its conceptual resources and research approaches, open the way for psychological knowledge and practice that are more culturally situated. PMID:24841336

  6. Cross-Cultural Adaptation of the CHAMPS Questionnaire in French Canadians with COPD

    Science.gov (United States)

    Mak, Susanne; Soicher, Judith E.; Mayo, Nancy E.; Wood-Dauphinee, Sharon; Bourbeau, Jean

    2016-01-01

    Physical activity is difficult to measure in individuals with COPD. The Community Healthy Activities Model Program for Seniors (CHAMPS) questionnaire demonstrated strong clinometric properties when used with the elderly and with those affected by chronic disease. Study objectives were to translate, culturally adapt the CHAMPS into French, and reexamine its test-retest reliability and construct validity in French and English Canadians with COPD. This paper presents the cross-cultural adaptation of the CHAMPS; results of its clinometric testing will be described in another article. The CHAMPS examines the degree of physical activity performed in a typical week through two summary scales, caloric expenditure and activity frequency. The CHAMPS was only in English; thus, a cross-cultural adaptation was needed to translate the CHAMPS into French for use in French Canadians with COPD. Cross-cultural adaptation consisted of forward and back translation, with expert review at each stage of translation: minor inconsistencies were uncovered and rectified. Five French participants with COPD completed the finalized Canadian French CHAMPS and participated in cognitive debriefing; no problematic items were identified. A structured and stepwise, cross-cultural adaptation process produced the Canadian French CHAMPS, with items of equivalent meaning to the English version, for use in French Canadians with COPD. PMID:27445570

  7. Cross-Cultural Adaptation of the CHAMPS Questionnaire in French Canadians with COPD

    Directory of Open Access Journals (Sweden)

    Susanne Mak

    2016-01-01

    Full Text Available Physical activity is difficult to measure in individuals with COPD. The Community Healthy Activities Model Program for Seniors (CHAMPS questionnaire demonstrated strong clinometric properties when used with the elderly and with those affected by chronic disease. Study objectives were to translate, culturally adapt the CHAMPS into French, and reexamine its test-retest reliability and construct validity in French and English Canadians with COPD. This paper presents the cross-cultural adaptation of the CHAMPS; results of its clinometric testing will be described in another article. The CHAMPS examines the degree of physical activity performed in a typical week through two summary scales, caloric expenditure and activity frequency. The CHAMPS was only in English; thus, a cross-cultural adaptation was needed to translate the CHAMPS into French for use in French Canadians with COPD. Cross-cultural adaptation consisted of forward and back translation, with expert review at each stage of translation: minor inconsistencies were uncovered and rectified. Five French participants with COPD completed the finalized Canadian French CHAMPS and participated in cognitive debriefing; no problematic items were identified. A structured and stepwise, cross-cultural adaptation process produced the Canadian French CHAMPS, with items of equivalent meaning to the English version, for use in French Canadians with COPD.

  8. A Study on the Origins of Cultural Films in Korea: A Focus on Films by the Japanese Government-General of Korea

    Directory of Open Access Journals (Sweden)

    Ryeosil Kim

    2014-08-01

    Full Text Available The term "cultural film" was coined in Korea under the Japanese rule, and it had been used as a general term for nonfiction films since the Korean liberation until the 1980s. However, unlike the term "documentary film," cultural film contains implications of propaganda film, controlled and produced as part of cultural and public relations policies of the government, thus it had been seldom studied for a long time. The term “cultural film” did not mean a film genre in the beginning. Therefore it is impossible to conceptualize the term based on common formats or contents of the films which have been called “cultural films.” Cultural films have been generally recognized as nonfiction films, and therefore documentary films. Yet in the production and distribution sites, fiction films and even animations were considered cultural films. In order to delve into the nature of cultural film, we have to reexamine the process in which the proper noun “Cultural Film” became a common noun. This paper attempts to explore the historical context in which the term “cultural film” was coined and used in the colonial Korea, when cultural films began to be produced. I examine the overall time period in which: 1 the concept of nonfiction film was first introduced in the Motion Picture Censorship Regulation, 2 the term “cultural film” became legally stipulated at a time when national policies were being established as Korea experienced the Manchurian Incident and the Sino-Japanese War, and 3 “cultural films” were screened mandatorily as “national films” that the nation had to foster through the Japan Film Law and Chosôn Film Act.

  9. A grounded theory of football fan community identity and co-production: Consumer roles in brand culture, meaning, and value co-creation in virtual communities

    OpenAIRE

    Healy, Jason C.

    2012-01-01

    This study theorizes why and how consumers consume. Using a combined methodology, drawing upon Netnography and Grounded Theory, to study an online fan forum called RedAndWhiteKop, this thesis considers brand culture/meaning and value co-creation. The research site is a VC containing football fans who are views as stakeholders of the organisation Liverpool Football Club. Following emergent fit with woven in literature streams found in managerial marketing as Service-Dominant Logic and the con...

  10. Culture, Parenting, and Zero-to-Threes

    Science.gov (United States)

    Bornstein, Marc H.

    2015-01-01

    One of the most important things that a parent does for a child is determine the culture into which that child is born. This article addresses parenting, culture, and the intersection of the two. The study of parenting in culture is one of similarities and differences in parental cognitions and practices and their meaning. The author provides…

  11. Cultural meanings of tuberculosis in Aceh Province, Sumatra.

    Science.gov (United States)

    Caprara, A; Abdulkadir, N; Idawani, C; Asmara, H; Lever, P; De Virgilio, G

    2000-07-01

    This paper shows how disease transmission and particularly what biomedicine calls tuberculosis are interpreted in the non-Western context of Aceh Province, Sumatra, Indonesia. It tries also to focus on factors influencing perceptions and health-seeking behaviors. Results show that what biomedicine calls TB is represented by a semantic network of illnesses. Parts of this network are clearly identified as transmissible while others are related to specific phenomena affecting the individual, such as terbuk (poisoning) or trouk (fatigue produced by hard work), and are not considered contagious. Forms of transmission are interpreted mostly through empirical and analogic categories. TB is attributed to four different aspects: (a) biomedical categories such as germ theory; (b) socio-economic conditions; (c) transgression of social rules; and (d) poisoning and the influences of supernatural powers. Health-seeking behaviors are related to the perceived causes of the disease, economic factors, and the accessibility of health services.

  12. Prospective study on cranioplasty with individual carbon fiber reinforced polymer (CFRP) implants produced by means of stereolithography.

    Science.gov (United States)

    Wurm, Gabriele; Tomancok, Berndt; Holl, Kurt; Trenkler, Johannes

    2004-12-01

    The aim of this study was to evaluate the value of carbon fiber reinforced polymer (CFRP) cranial implants produced by means of 3-dimensional (3D) stereolithography (SL) and template modeling for reconstructions of complex or extensive cranial defects. A series of 41 cranioplasties with individual CFRP implants was performed in 37 patients between April 1996 and November 2002. Only patients with complex and/or large cranial defects were included, most of them having extended scarring or dural calcification and poor quality of the overlying soft-tissue cover after infection or multiple preceding operations. Involvement of frontal sinus, a known risk factor for complications after cranioplasty, was the case in 21 patients (51.2%). A computer-based 3D model of the skull with the bony defect was generated by means of stereolithography after acquisition, evaluation and transfer of the patient's helical computed tomography (CT) data. A wax template of the defect that was used to design the individual prosthesis-shape was invested in dental stone. Then, the cranial implant was fabricated out of CFRP by loosen mold. Reconstruction of defects measuring up to 17 x 9 cm was performed. The intra-operative fit of the implants was excellent in 36 (87.8%), good in 1 (2.4%), and fair in 4 (9.8%) of the cases. Problems of implant fit occurred because of extended scarring and poor quality of soft-tissue cover. Adverse reactions were observed in 5 patients (1 subdural, 1 subcutaneous hematoma, 2 infections, 1 allergic reaction). Excellent contours and a solid stable reconstruction have been maintained in 30 out of 35 remaining plates (mean follow-up 3.6 years). No adverse effects concerning postoperative imaging, the accuracy of electroencephalograms and radiation therapy have been observed. The authors believe that this relatively new technique represents an advance in the management of complex and large cranial defects, but seems less suitable for simple defects because of cost

  13. The cultural animal human nature, meaning, and social life

    CERN Document Server

    Baumeister, Roy F

    2005-01-01

    What makes us human? Why do people think, feel, and act as they do? What is the essence of human nature? What is the basic relationship between the individual and society? These questions have fascinated both great thinkers and ordinary humans for centuries. Now, at last, there is a solid basis for answering them, in the form of the accumulated efforts and studies by thousands of psychology researchers. We no longer have to rely on navel-gazing and speculation to understand why people are the way they are - we can instead turn to solid, objective findings. This book, by an eminent social psychologist at the peak of his career, not only summarizes what we know about people - it also offers a coherent, easy-to-understand, through radical, explanation. Turning conventional wisdom on its head, the author argues that culture shaped human evolution. Contrary to theories that depict the individual's relation to society as one of victimization, endless malleability, or just a square peg in a round hole, he proposes t...

  14. Teaching of History: argumentation and the construction of meaning

    Directory of Open Access Journals (Sweden)

    Patricia Bastos de Azevedo

    2011-06-01

    Full Text Available The aim of this article is to discuss the construction of meaning through argumentation in the teaching of History. The field research was carried out in a municipal school in Niterói – RJ. The research included 30 hours of recordings of History classes of a group of the 4th cycle of Elementary Education (also called 8th year of Elementary Education. The data analysis articulated the theoretical elements related to curriculum, language and culture. It can be pointed out a possibility of theoretical approximation of these three fields of knowledge relating them to the concrete space of the History class. The challenge is to create a dialogue among the theories from the curriculum, language and culture fields, aiming to understand the construction of meaning in the teaching of History by means of argumentation.

  15. Isolation and morphological characterization of antibiotic producing ...

    African Journals Online (AJOL)

    Purpose: To isolate and characterize antibiotic producing actinomycetes from soil samples in Belgaum, Karnataka, India. Methods: Crowded plate technique was used for the isolation of actinomycetes in media such as soybean – casein digest medium and actinomycetes isolation agar. The morphological and cultural ...

  16. Isolation of biosurfactant producers, optimization and properties of biosurfactant produced by Acinetobacter sp. from petroleum-contaminated soil.

    Science.gov (United States)

    Chen, J; Huang, P T; Zhang, K Y; Ding, F R

    2012-04-01

    To screen and identify biosurfactant producers from petroleum-contaminated soil; to use response surface methodology (RSM) for medium optimization to enhance biosurfactant production; and to study the properties of the newly obtained biosurfactant towards pH, temperature and salinity. We successfully isolated three biosurfactant producers from petroleum-contaminated soil and identified them through 16S rRNA sequence analysis, which exhibit the highest similarities to Acinetobacter beijerinckii (100%), Kocuria marina (99%) and Kineococcus marinus (99%), respectively. A quadratic response model was constructed through RSM designs, leading to a 57·5% increase of the growth-associated biosurfactant production by Acinetobacter sp. YC-X 2 with an optimized medium: beef extract 3·12 g l(-1) ; peptone 20·87 g l(-1) ; NaCl 1·04 g l(-1); and n-hexadecane 1·86 g l(-1). Biosurfactant produced by Acinetobacter sp. YC-X 2 retained its properties during exposure to a wide range of pH values (5-11), high temperatures (up to 121°C) and high salinities [up to 18% (w/v) Na(+) and Ca(2+) ], which was more sensitive to Ca(2+) than Na(+). Two novel biosurfactant producers were isolated from petroleum-contaminated soil. Biosurfactant from Acinetobacter sp. YC-X 2 has good properties to a wide range of pH, high temperature and high salinity, and its production was optimized successfully through RSM. The fact, an increasing demand of high-quality surfactants and the lack of cost-competitive bioprocesses of biosurfactants for commercial utilization, motivates researchers to develop cost-effective strategies for biosurfactant production through isolating new biosurfactant producers with special surface-active properties and optimizing their cultural conditions. Two novel biosurfactant producers in this study will widen our knowledge about this kind of micro-organism. This work is the first application of RSM designs for cultural optimization of biosurfactant produced by Acinetobacter

  17. A Comparison of the Cultural

    Science.gov (United States)

    Hwang, Eun Jin

    2012-01-01

    As a critical unit for identifying family-constructed meanings of education, a deeper contextual understanding of Korean immigrant parents' cultural/ethnic perceptions in relation to educational beliefs should be central to culturally responsive education designed to support Korean immigrant families. It is necessary for educators to examine…

  18. A new taxol-producing fungus ( Pestalotiopsis malicola ) and ...

    African Journals Online (AJOL)

    A new taxol-producing fungus ( Pestalotiopsis malicola ) and evidence for taxol as a transient product in the culture. ... African Journal of Biotechnology. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives.

  19. Training on the DSM-5 Cultural Formulation Interview improves cultural competence in general psychiatry residents: A pilot study.

    Science.gov (United States)

    Mills, Stacia; Xiao, Anna Q; Wolitzky-Taylor, Kate; Lim, Russell; Lu, Francis G

    2017-04-01

    The objective of this study was to assess whether a 1-hour didactic session on the DSM-5 Cultural Formulation Interview (CFI) improves the cultural competence of general psychiatry residents. The main hypothesis was that teaching adult psychiatry residents a 1-hour session on the CFI would improve cultural competence. The exploratory hypothesis was that trainees with more experience in cultural diversity would have a greater increase in cultural competency scores. Psychiatry residents at a metropolitan, county hospital completed demographics and preintervention questionnaires, were exposed to a 1-hour session on the CFI, and were given a postintervention questionnaire. The questionnaire was an adapted version of the validated Cultural Competence Assessment Tool . Paired samples t tests compared pre- to posttest change. Hierarchical linear regression assessed whether pretraining characteristics predicted posttest scores. The mean change of total pre- and posttest scores was significant ( p = .002), as was the mean change in subscales Nonverbal Communications ( p < .001) and Cultural Knowledge ( p = .002). Demographic characteristics did not predict higher posttest scores (when covarying for pretest scores). Psychiatry residents' cultural competence scores improved irrespective of previous experience in cultural diversity. More research is needed to further explore the implications of the improved scores in clinical practice.

  20. Volatile compounds in samples of cork and also produced by selected fungi.

    Science.gov (United States)

    Barreto, M C; Vilas Boas, L; Carneiro, L C; San Romão, M V

    2011-06-22

    The production of volatile compounds by microbial communities of cork samples taken during the cork manufacturing process was investigated. The majority of volatiles were found in samples collected at two stages: resting after the first boiling and nontreated cork disks. Volatile profiles produced by microbiota in both stages are similar. The releasable volatile compounds and 2,4,6-trichloroanisole (TCA) produced in cork-based culture medium by five isolated fungal species in pure and mixed cultures were also analyzed by gas chromatography coupled with mass spectrometry (GC-MS).The results showed that 1-octen-3-ol and esters of fatty acids (medium chain length C8-C20) were the main volatile compounds produced by either pure fungal species or their mixture. Apparently, Penicillium glabrum is the main contributor to the overall volatile composition observed in the mixed culture. The production of releasable TCA on cork cannot be attributed to any of the assayed fungal isolates.

  1. 1. Culture, Heritage, and Ethics

    OpenAIRE

    Sandis, Constantine

    2016-01-01

    Theory without practice is empty, practice without theory is blind, to adapt a phrase from Immanuel Kant. The sentiment could not be truer of cultural heritage ethics. This intra-disciplinary book bridges the gap between theory and practice by bringing together a stellar cast of academics, activists, consultants, journalists, lawyers, and museum practitioners, each contributing their own expertise to the wider debate of what cultural heritage means in the twenty-first century. Cultural Herit...

  2. The cultural context of development: language as a means for thinking and problem-solving

    Directory of Open Access Journals (Sweden)

    Azwihangwisi Muthivhi

    2011-12-01

    Full Text Available The present study, located in the socio-cultural tradition of research in developmental psychology, uses experimental tasks, adapted from the groundbreaking Lurian study (Luria, 1979, 1976 to investigate South African children’s acquisition and development of thinking and concepts – involving classification and generalisation, and how these concepts are linked to the specific cultural context of their manifestation.The paper provides new ways of understanding possible causes of contemporary problems that children encounter during classroom learning by examining the developmental roots of the specific modes of thinking and concept development in their concrete learning and developmental settings and specific tradition of learning within their schooling.

  3. How can you capture cultural dynamics?

    Directory of Open Access Journals (Sweden)

    Yoshihisa eKashima

    2014-09-01

    Full Text Available Cross-cultural comparison is a critical method by which we can examine the interaction between culture and psychological processes. However, comparative methods tend to overlook cultural dynamics – the formation, maintenance, and transformation of cultures over time. The present article gives a brief overview of four different types of research designs that have been used to examine cultural dynamics in the literature: (1 cross-temporal methods that trace medium- to long-term changes in a culture; (2 cross-generational methods that explore medium-term implications of cultural transmission; (3 experimental simulation methods that investigate micro-level mechanisms of cultural dynamics; and (4 formal models and computer simulation methods often used to investigate long-term and macro-level implications of micro-level mechanisms. These methods differ in terms of level of analysis for which they are designed (micro vs. macro-level, scale of time for which they are typically used (short-, medium-, or long-term, and direction of inference (deductive vs. empirical method that they imply. The paper describes examples of these methods, discuss their strengths and weaknesses, and point to their complementarity in inquiries about cultural change. Because cultural dynamics research is about meaning over time, issues deriving from interpretation of meaning and temporal distance between researchers and objects of inquiry can pose threats to the validity of the research and its findings. The methodological question about hermeneutic circle is recalled and further inquiries are encouraged.

  4. How can you capture cultural dynamics?

    Science.gov (United States)

    Kashima, Yoshihisa

    2014-01-01

    Cross-cultural comparison is a critical method by which we can examine the interaction between culture and psychological processes. However, comparative methods tend to overlook cultural dynamics – the formation, maintenance, and transformation of cultures over time. The present article gives a brief overview of four different types of research designs that have been used to examine cultural dynamics in the literature: (1) cross-temporal methods that trace medium- to long-term changes in a culture; (2) cross-generational methods that explore medium-term implications of cultural transmission; (3) experimental simulation methods that investigate micro-level mechanisms of cultural dynamics; and (4) formal models and computer simulation methods often used to investigate long-term and macro-level implications of micro-level mechanisms. These methods differ in terms of level of analysis for which they are designed (micro vs. macro-level), scale of time for which they are typically used (short-, medium-, or long-term), and direction of inference (deductive vs. empirical method) that they imply. The paper describes examples of these methods, discuss their strengths and weaknesses, and point to their complementarity in inquiries about cultural change. Because cultural dynamics research is about meaning over time, issues deriving from interpretation of meaning and temporal distance between researchers and objects of inquiry can pose threats to the validity of the research and its findings. The methodological question about hermeneutic circle is recalled and further inquiries are encouraged. PMID:25309476

  5. LINGUISTIC AND CULTURAL STUDIES: THE QUEST FOR NEW IDEAS

    Directory of Open Access Journals (Sweden)

    Vitalii Kononenko

    2015-05-01

    Full Text Available The article highlights the principles of researching into text from the interdisciplinary linguistic and cultural perspective. Cognitological analysis of linguistic and extralinguistic cultural meanings reveals that there exist of specific linguistic and aesthetic formations best presented through the ‘language – culture – identity’ triad. One of the components of literary discourse is monocultural layer, which secures the continuity of national cultural tradition; researching into it, one should take into account mental and historical, psycholinguistic, sociolinguistic and other factors. Linguistic and aesthetic analysis helps to establish the system of linguistic and cultural means (metaphorization, imagery, verbal symbols, linguistic conceptualization, connotative meanings, which reveals its potential in literary texts. The lingual identity as a general notional category shows its nationally-oriented characteristics through the dichotomies of ‘addresser-addressee’ , ‘author-reader’, ‘narrator-narratee’ and is presented in the author’s idiolect.

  6. The influence of culture of honor and emotional intelligence in the acculturation of Moroccan immigrant women.

    Science.gov (United States)

    Lopez-Zafra, Esther; El Ghoudani, Karima

    2014-01-01

    Migration is a normal process of people seeking new opportunities, work, or leisure in societies. The way people adapt to a new country (acculturation) is a complex process in which immigrants' evaluations about the culture of origin and their perceptions of the host country interact. The combination of these two factors produces four types of acculturation: separation, assimilation, integration, and marginalization. Several variables, such as personality, attitudes, and emotional intelligence, have been studied to help explain this process. However, the impact of a culture of honor and its interaction with other variables remains an open question that may help to explain how migrants can better adjust to their host culture. In this study, we examine the influence of the culture of honor (social) and emotional intelligence (individual) on acculturation. In a sample of 129 Moroccan women (mean age = 29, SD = 9.40) immigrants in Spain (mean time in Spain = 6 years, SD = 3.60), we investigated the relations among the variables of interest. Our results show that no significant differences emerged in the scores given for culture of honor (CH) and the acculturation strategies of the Moroccan immigrant women F(3, 99) = .233; p = .87. However women who preferred the integration strategy scored highest on emotional intelligence (EI), whereas the assimilated immigrants showed the lowest scores for EI F(3, 92) = 4.63; p = .005. Additionally, only in the case of integration does EI mediate between CH and the value given to the immigrant's own and host cultures (p <.001).

  7. Aleuria aurantia - indole metabolites of fruit bodies, mycelial culture and culture medium

    Directory of Open Access Journals (Sweden)

    Janina Węgiel

    2014-08-01

    Full Text Available The aim of present study was to investigate and compare indole metabolites of fruit bodies, mycelium cultivated in vitro and culture medium of the fungus Aleuria aurantia (Fr. Fuck. By use of a number of chromatographic and spectroscopic methods several indole metabolites have been detected and identified among other the 3-indolebutyric acid was produced and extracted to the culture medium. Furthermore 3-indoleatonitrile and tryptophane degradative products have been found both in fruit bodies and mycelium.

  8. Musical meaning and social significance : techno triggers for dancing

    OpenAIRE

    Gadir, Tami Ester

    2014-01-01

    Electronically-produced dance music has only recently achieved as much visibility in the global pop music industry as ‘live’ or instrumental pop. Yet the fascination of cultural scholars and sociologists with dance music predates its rise as a product of mass culture. Much of this interest derives from early associations of dance music with marginalised groups and oppositional ideologies. It therefore follows that many explorations of dance music focus on the ways in which tech...

  9. Cultural Practices, Oppression, and Morality.

    Science.gov (United States)

    Turiel, Elliot

    1998-01-01

    Argues that contested meanings, multiple judgments, and conflicts are part of cultures and the individual's thoughts and actions. Contends that people make moral judgments that may affirm or contradict cultural norms and practices, and sometimes invoke concepts of welfare, justice, and rights. Notes that some key aspects of Baumrind's neo-Marxist…

  10. Space, Culture and Power in Buchi Emecheta’s Novel Called Kehinde

    Directory of Open Access Journals (Sweden)

    Ayla OĞUZ

    2017-12-01

    Full Text Available When it is considered related with power, possible meanings of space and culture gain a more importance. In postcolonial literary theory, power is introduced to be something in the hands of the people belonging to Western world. Due to this, the colonizer’s cultural knowledge about the colonized’s culture and space determines the social rank and location of non-Western people. Cultural superiority of western world over non-western world is formed due to power- knowledge relationship in postcolonial literary theory. According to Gayatri Chakravorty Spivak, knowledge in postcolonial context isn’t guiltless because it is the output of its producers. Thus, dominant power gives a new shape to such concepts like space, culture and power belonging to subordinate people. Within this context Buchi Emecheta’s interesting novel called Kehinde tells the story of a couple trying to decide on staying in London and returning to their own country, Nigeria. In the novel Kehinde as a female and her husband Albert as a male experience the multifaceted influence of both living in the center and belonging to periphery and reflect the influence of space, culture and power in their life, family and social circumference. Hence, it is anayzed in the study how Emecheta tries to show the reader the fact that power dominates everything and causes complication in man’s life referring to postcolonial literary theory while constructing the postcolonial identies in the novel.

  11. Long-term brain slice culturing in a microfluidic platform

    DEFF Research Database (Denmark)

    Vedarethinam, Indumathi; Avaliani, N.; Tønnesen, J.

    2011-01-01

    In this work, we present the development of a transparent poly(methyl methacrylate) (PMMA) based microfluidic culture system for handling long-term brain slice cultures independent of an incubator. The different stages of system development have been validated by culturing GFP producing brain sli...

  12. Experimental investigation on spontaneously active hippocampal cultures recorded by means of high-density MEAs: analysis of the spatial resolution effects

    Directory of Open Access Journals (Sweden)

    Alessandro Maccione

    2010-05-01

    Full Text Available Based on experiments performed with high-resolution Active Pixel Sensor microelectrode arrays (APS-MEAs coupled with spontaneously active hippocampal cultures, this work investigates the spatial resolution effects of the neuroelectronic interface on the analysis of the recorded electrophysiological signals. The adopted methodology consists, first, in recording the spontaneous activity at the highest spatial resolution (inter-electrode separation of 21 µm from the whole array of 4096 microelectrodes. Then, the full resolution dataset is spatially down sampled in order to evaluate the effects on raster plot representation, array-wide spike rate (AWSR, mean firing rate (MFR and mean bursting rate (MBR. Furthermore, the effects of the array-to-network relative position are evaluated by shifting a subset of equally spaced electrodes on the entire recorded area. Results highlight that MFR and MBR are particularly influenced by the spatial resolution provided by the neuroelectronic interface. On high-resolution large MEAs, such analysis better represent the time-based parameterization of the network dynamics. Finally, this work suggest interesting capabilities of high-resolution MEAs for spatial-based analysis in dense and low-dense neuronal preparation for investigating signalling at both local and global neuronal circuitries.

  13. Production of highly knotted DNA by means of cosmid circularization inside phage capsids

    Directory of Open Access Journals (Sweden)

    Trigueros Sonia

    2007-12-01

    Full Text Available Abstract Background The formation of DNA knots is common during biological transactions. Yet, functional implications of knotted DNA are not fully understood. Moreover, potential applications of DNA molecules condensed by means of knotting remain to be explored. A convenient method to produce abundant highly knotted DNA would be highly valuable for these studies. Results We had previously shown that circularization of the 11.2 kb linear DNA of phage P4 inside its viral capsid generates complex knots by the effect of confinement. We demonstrate here that this mechanism is not restricted to the viral genome. We constructed DNA cosmids as small as 5 kb and introduced them inside P4 capsids. Such cosmids were then recovered as a complex mixture of highly knotted DNA circles. Over 250 μg of knotted cosmid were typically obtained from 1 liter of bacterial culture. Conclusion With this biological system, DNA molecules of varying length and sequence can be shaped into very complex and heterogeneous knotted forms. These molecules can be produced in preparative amounts suitable for systematic studies and applications.

  14. Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing Bacterium Bacillus megaterium SP1 and Its Application in Aquaculture Wastewater Treatment.

    Science.gov (United States)

    Luo, Liang; Zhao, Zhigang; Huang, Xiaoli; Du, Xue; Wang, Chang'an; Li, Jinnan; Wang, Liansheng; Xu, Qiyou

    2016-01-01

    A bioflocculant-producing bacterium, Bacillus megaterium SP1, was isolated from biofloc in pond water and identified by using both 16S rDNA sequencing analysis and a Biolog GEN III MicroStation System. The optimal carbon and nitrogen sources for Bacillus megaterium SP1 were 20 g L -1 of glucose and 0.5 g L -1 of beef extract at 30°C and pH 7. The bioflocculant produced by strain SP1 under optimal culture conditions was applied into aquaculture wastewater treatment. The removal rates of chemical oxygen demand (COD), total ammonia nitrogen (TAN), and suspended solids (SS) in aquaculture wastewater reached 64, 63.61, and 83.8%, respectively. The volume of biofloc (FV) increased from 4.93 to 25.97 mL L -1 . The addition of Bacillus megaterium SP1 in aquaculture wastewater could effectively improve aquaculture water quality, promote the formation of biofloc, and then form an efficient and healthy aquaculture model based on biofloc technology.

  15. DIAGNOSIS OF CULTURAL ORGANIZATIONS

    Directory of Open Access Journals (Sweden)

    ALBU MĂDĂLINA

    2015-04-01

    Full Text Available Cultural institution management is to direct the organization to a specific cultural profile purpose, namely production values esthetic sense, artistic, moral, spiritual, distribution, promotion of these values, protection and circulation of cultural heritage. In this regard, an analysis in the diagnosis cultural organizations aims to determine the main strengths and weaknesses, assess the potential and making recommendations focused on the root causes of failures and positive aspects. This paper presents considerations diligence activity Culture House "IL Caragiale "in Ploiesti. The mission of this organization is to contribute to the cultural development of the community by initiating projects and cultural programs, offer development programs and services to meet cultural needs, increase public access to diverse cultural life, providing a constant presence institution circuit local, national, European and international level. Conclusions drawn from the analysis shows that in a world of economic globalization, information and culture in a company in constant change, in a competitive market where there is information readily available means and leisure, but not cultural consistency in a social environment where interest in culture of people is declining, the situation of the population is impaired, the remuneration of staff working in the field of cultural education is demotivating, the funds allocated to culture have grown lately effectively lead a cultural institution is a challenge.

  16. Gender in identification practices of mass culture

    Directory of Open Access Journals (Sweden)

    O. B. Sinkevych

    2014-10-01

    The dynamics of the images of masculinity and femininity in popular culture depends on shared cultural paradigm. However, these images not only reflect, but also construct social and cultural reality. Gender representation in popular culture activates the process of selecting, structuring and formation of values of a stereotype, its communicative refinement, giving it new meaning. It promotes innovative images, which play the role of landmarks gender identity.

  17. Developing the Pedagogical Culture of Parents by Means of Social Partnership with a Supplementary Education Institution

    Science.gov (United States)

    Zakirova, Venera G.; Nikitina, Ekaterina L.

    2016-01-01

    The urgency of the research is due to the current requirements to develop the pedagogical culture of parents, which can help them to be successful parents, to effectively interact with their children avoiding family upbringing mistakes. In this respect, the article aims to identify the conditions for development of the pedagogical culture of…

  18. Host cells and methods for producing isoprenyl alkanoates

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Taek Soon; Fortman, Jeffrey L.; Keasling, Jay D.

    2015-12-01

    The invention provides for a method of producing an isoprenyl alkanoate in a genetically modified host cell. In one embodiment, the method comprises culturing a genetically modified host cell which expresses an enzyme capable of catalyzing the esterification of an isoprenol and a straight-chain fatty acid, such as an alcohol acetyltransferase (AAT), wax ester synthase/diacylglycerol acyltransferase (WS/DGAT) or lipase, under a suitable condition so that the isoprenyl alkanoate is produced.

  19. Skip the (Capitalist Intermediaries: Freedom, Democracy, and Participation in the Economy and Culture

    Directory of Open Access Journals (Sweden)

    Forrest Perry

    2015-11-01

    Full Text Available A significant parallel can be drawn between a socialist argument about how to regulate property in the means of production and the liberal theorist Lawrence Lessig’s argument about how to regulate property in ideas. The two are particularly alike in the emphasis they place on the kind of participation—and the resulting freedom and democracy—enabled by having access to the means of production. Highlighting the parallel could help socialists and liberal theorists of intellectual property to see how they can advance each other’s causes: socialism could eliminate the threat of privatization of the means of producing culture that liberals like Lessig rightly worry about; and if the arguments about ownership of ideas made by Lessig and others like him end up gaining currency, then arguments about ownership of the means of production made by socialist theorists could have more resonance with larger numbers of people than is currently the case.

  20. Method of producing molybdenum-99

    Science.gov (United States)

    Pitcher, Eric John

    2013-05-28

    Method of producing molybdenum-99, comprising accelerating ions by means of an accelerator; directing the ions onto a metal target so as to generate neutrons having an energy of greater than 10 MeV; directing the neutrons through a converter material comprising techentium-99 to produce a mixture comprising molybdenum-99; and, chemically extracting the molybdenum-99 from the mixture.

  1. Structure-Activity Relationships of Bioengineered Heparin/Heparan Sulfates Produced in Different Bioreactors

    Directory of Open Access Journals (Sweden)

    Ha Na Kim

    2017-05-01

    Full Text Available Heparin and heparan sulfate are structurally-related carbohydrates with therapeutic applications in anticoagulation, drug delivery, and regenerative medicine. This study explored the effect of different bioreactor conditions on the production of heparin/heparan sulfate chains via the recombinant expression of serglycin in mammalian cells. Tissue culture flasks and continuously-stirred tank reactors promoted the production of serglycin decorated with heparin/heparan sulfate, as well as chondroitin sulfate, while the serglycin secreted by cells in the tissue culture flasks produced more highly-sulfated heparin/heparan sulfate chains. The serglycin produced in tissue culture flasks was effective in binding and signaling fibroblast growth factor 2, indicating the utility of this molecule in drug delivery and regenerative medicine applications in addition to its well-known anticoagulant activity.

  2. ENVIRONMENTAL PHILOSOPHY AND ECOLOGICAL CULTURE

    Directory of Open Access Journals (Sweden)

    Kalimat M. Alilova

    2017-01-01

    Full Text Available Aim. The aim of the research is to study environmental problems related to the decline of culture, the importance of philosophy in overcoming private and personal interests as well as the unilateral approach of man in his relationship to nature. The study shows how philosophy can participate in the formation of ecological culture, a new ecological consciousness in man, while ecological culture is called upon to resist technocratic stereotypes and the course of history was aimed at preventing the biosphere from becoming deserted. Discussion. On the basis of the analysis of literary sources, we used the method of socio-cultural and socio-natural approaches based on the possibility of philosophy to introduce a new life into culture, new ecological values and new ecological principles. To solve these problems, environmental philosophy develops new theories. Representatives of different cultures, ethnic groups, nations, religions must learn to coexist with each other. We consider philosophy as a means of teaching rapprochement between peoples and creating new opportunities for understanding and improving the environmental situation. Cultural development makes it possible to assess the level of a man’s knowledge of nature, himself and the world around him. Ecological culture is a way of connecting man with nature on the basis of deeper knowledge and understanding. Philosophy says that you cannot move away from nature and be lauded over it since this will destroy culture. Rational doctrines tend to put a person above other living beings so the synthesis of philosophy with culture can have a positive ecological meaning. Conclusion. The findings obtained can be recommended for practical use in schools, starting from primary school, as well as in secondary special educational institutions and universities. It is necessary to work on the motivation and values of people, develop a common and ecological culture. Only a cultured person can move from

  3. The Influence of Culture on Anxiety in Latino Youth: A Review

    Science.gov (United States)

    Varela, R. Enrique; Hensley-Maloney, Lauren

    2009-01-01

    This article reviews the literature on how culture influences anxiety in Latino youth. First, a review of cross-cultural variations in prevalence and measurement is presented. Then, the article focuses on how culture impacts the meaning and expression of anxiety. Specifically, we discuss the meaning and expression of anxiety, the impact of culture…

  4. Preparation of Biocomposites using Sawdust and Lignosulfonate with a Culturе Liquid of Levan Producer Azotobacter vinelandii as a Bonding Agent

    Directory of Open Access Journals (Sweden)

    Victor V. Revin

    2016-02-01

    Full Text Available The possibility of preparing molding-bioengineered materials, such as woodchip boards (WCB, from sawdust using technical lignosulfonate (LGS, a wood waste product, and a culture liquid (CL of levan microbial polysaccharide producer by Azotobacter vinelandii D-08 is explored in this article. The parameters of the derived materials are comparable to those of traditional materials made from toxic phenol-formaldehyde resins. The various physical and mechanical characteristics of the materials depend on the quantity of the bonding agent used for the preparation. Adding a culture liquid increases the humidity resistance of the molding materials. Using electron microscopy and X-ray micro-tomography, it is clear that the structure of woodchip boards become more homogeneous without microcracks with the addition of CL. The strength of the best samples prepared was approximately 24 to 29 MPa with a density of 1170 to 1255 kg/m3 and a swell on wetting of 6.7%. During hot pressing, noticeable changes were observed by Fourier transform infrared spectroscopy (FTIR at frequencies typical of LGS sulfonic-acid groups, levan fructose fragments, and skeletal vibrations of a syringal/guaiacyl core in lignin and of C-H groups of hemicelluloses. This indicates the involvement of these functional groups in the process of binding wood particles with hot pressing.

  5. Organelles genome stability of wheat plantlets produced by anther ...

    African Journals Online (AJOL)

    Yomi

    2012-03-15

    Mar 15, 2012 ... 1Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa' Applied University, ... genetic stability of wheat organelles genomes for plantlets produced by anther culture using restriction ..... of transgenic plants.

  6. Objective and character of safety culture

    International Nuclear Information System (INIS)

    Aastrand, K.

    2005-01-01

    The main topics of the lecture include: (1) concepts of safety culture introduced in INSAG-4, (2) stages of development of safety culture, (3) general practises to develop organizational effectiveness as a means of implementing and improving safety culture, (4) specific practises to develop safety culture applying to all stages of a nuclear installation's life cycle, (5) suggestions on assessing the progress of development of safety culture in an organization and on evaluating the influence of major environmental and internal organizational factors on that culture, and (6) guidance on the detection of incipient weaknesses in safety culture that may be of particular interest to regulators and those responsible for self-assessment in organization

  7. The Intersection of Culture and Achievement Motivation

    Science.gov (United States)

    Trumbull, Elise; Rothstein-Fisch, Carrie

    2011-01-01

    Achievement motivation is something that all members of the school community want to support in students, however few may recognize that it is influenced by culture. The very meaning of "achievement" is culturally variable, and the motives that students have for achieving may be quite different, depending upon their cultural background.…

  8. Perspectives in geography of culture and civilizations

    Directory of Open Access Journals (Sweden)

    Grčić Мirko

    2014-01-01

    Full Text Available This paper presents a comparative analysis of relevant methodological essence of "traditional" and "new" cultural geography. In the introduction is given an explanation of philosophic concepts of space, environment, place and the region in cultural geography. In second section is analyzed the meaning of civilization and the genesis of geography of civilization (géographie de civilisation. Special attention is on features of geographical posibilism as methodological paradigm, and the concept of cultural landscape as the essence of classical geography of culture and civilization. After this part are researched specific characteristics of certain academic schools and methodological perspectives in cultural geography. Postmodern paradigm and essence of "new" cultural geography are in the main focus. Postmodernism is changing the meaning of the basic concepts in cultural geography, which are analyzed in the introduction, such as space, culture, cultural region, cultural landscape and others. "New" cultural geography reassessed social and moral issues associated with the characteristics of the postmodern era. In this regard, methodological paradigm must be changed. This ascertainment is based on the interpretation of humanistic geography, where the emphasis is on the interpretation of cultural symbols, causal link and the "spirit of place" (Spiritus Loci. In accordance with modern conceptions of human in psychological notion, there are at least three theoretical directions, which find resonance in the appropriate cultural geography: behaviorism, psychoanalytic concept and cognitive concept - gestaltism and geography of perception. In conclusion is emphasized the need of finding a dialectical unity in "classical" and "new" cultural geography. [Projekat Ministarstva nauke Republike Srbije, br. 176017

  9. Targeting development of incretin-producing cells increases insulin secretion

    DEFF Research Database (Denmark)

    Petersen, Natalia; Reimann, Frank; van Es, Johan H

    2015-01-01

    the number of intestinal L cells, which produce GLP-1, is an alternative strategy to augment insulin responses and improve glucose tolerance. Blocking the NOTCH signaling pathway with the γ-secretase inhibitor dibenzazepine increased the number of L cells in intestinal organoid-based mouse and human culture...... of the development of incretin-producing cells in the intestine has potential as a therapeutic strategy to improve glycemic control....

  10. 3D+time acquisitions of 3D cell culture by means of lens-free tomographic microscopy

    Science.gov (United States)

    Berdeu, Anthony; Laperrousaz, Bastien; Bordy, Thomas; Morales, S.; Gidrol, Xavier; Picollet-D'hahan, Nathalie; Allier, Cédric

    2018-02-01

    We propose a three-dimensional (3D) imaging platform based on lens-free microscopy to perform multi-angle acquisitions on 3D cell cultures embedded in extracellular matrix (ECM). We developed algorithms based on the Fourier diffraction theorem to perform fully 3D reconstructions of biological samples and we adapted the lens-free microscope to incubator conditions. Here we demonstrate for the first time, 3D+time lens-free acquisitions of 3D cell culture over 8 days directly into the incubator. The 3D reconstructed volume is as large as 5 mm3 and provides a unique way to observe in the same 3D cell culture experiment multiple cell migration strategies. Namely, in a 3D cell culture of prostate epithelial cells embedded within a Matrigel® matrix, we are able to distinguish single cell 'leaders', migration of cell clusters, migration of large aggregates of cells, and also close-gap and large-scale branching. In addition, we observe long-scale 3D deformations of the ECM that modify the geometry of the 3D cell culture. Interestingly, we also observed the opposite, i.e. we found that large aggregates of cells may deform the ECM by generating traction forces over very long distances. In sum we put forward a novel 3D lens-free microscopy tomographic technique to study the single and collective cell migrations, the cell-to-cell interactions and the cell-to-matrix interactions.

  11. Transcultural nursing: providing culturally congruent care to the Hausa of Northwest Africa.

    Science.gov (United States)

    Chmielarczyk, V

    1991-01-01

    Research around the world is now beginning to validate the theory of Cultural Care as an important means to provide culturally congruent care to clients, families, and groups of diverse cultures. Knowledge of Leininger's Theory of Cultural Care Diversity and Universality can provide meaningful care to clients who have different traditional and current beliefs and values. The Leininger Sunrise Model can serve as a valuable guide to discover care meanings and practices related to the theory, and to provide practical and meaningful culture specific care decisions and actions by nurses. The three major modes of action, namely, cultural care maintenance or preservation, accommodation or negotiation, and repatterning or restructuring, are important differential means to provide culturally congruent care to clients within their own cultural setting. This article considers the application of such care for the Hausa of Northwest Africa.(ABSTRACT TRUNCATED AT 250 WORDS)

  12. Museology and heritage in contemporary cities: a thesis on city management from the perspective of culture and memory preservation

    Directory of Open Access Journals (Sweden)

    Heloisa Helena Fernandes Gonçalves da Costa

    2012-04-01

    Full Text Available Essentialy considered a space to live, the contemporary cities are complexes territories where memory and heritage have no effective representative function. The main objective of this paper is to analyze the emergence of the 'cultural health', a new concept supported by the relationship between museology, heritage and citizenship. The research was based on fieldwork experience and on memory studies of adults and elderly people that live in urban areas, where they can observe the symbolic meaning of the monuments and similars objects within the museum cities and the cultural landscapes. The way that museums and monuments are inserted in civil society and their power to produce values and cultural codes make them part of the process to promote identities and citizenship. Also, this process can improve the cultural health, the self-esteem and the quality of social interaction. These elements are essential for the building of healthy cities.

  13. "The group facilitates everything": meanings patients with type 2 diabetes mellitus assigned to health education groups.

    Science.gov (United States)

    de Melo, Lucas Pereira; de Campos, Edemilson Antunes

    2014-01-01

    to interpret the meanings patients with type 2 diabetes mellitus assign to health education groups. ethnographic study conducted with Hyperdia groups of a healthcare unit with 26 informants, with type 2 diabetes mellitus, and having participated in the groups for at least three years. Participant observation, social characterization, discussion groups and semi-structured interviews were used to collect data. Data were analyzed through the thematic coding technique. four thematic categories emerged: ease of access to the service and healthcare workers; guidance on diabetes; participation in groups and the experience of diabetes; and sharing knowledge and experiences. The most relevant aspect of this study is the social use the informants in relation to the Hyperdia groups under study. the studied groups are agents producing senses and meanings concerning the process of becoming ill and the means of social navigation within the official health system. We expect this study to contribute to the actions of healthcare workers coordinating these groups given the observation of the cultural universe of these individuals seeking professional care in the various public health care services.

  14. The cultural psychology endeavor to make culture central to psychology: Comment on Hall et al. (2016).

    Science.gov (United States)

    Dvorakova, Antonie

    2016-12-01

    When Hall, Yip, and Zárate (2016) suggested that cultural psychology focused on reporting differences between groups, they described comparative research conducted in other fields, including cross-cultural psychology. Cultural psychology is a different discipline with methodological approaches reflecting its dissimilar goal, which is to highlight the cultural grounding of human psychological characteristics, and ultimately make culture central to psychology in general. When multicultural psychology considers, according to Hall et al., the mechanisms of culture's influence on behavior, it treats culture the same way as cross-cultural psychology does. In contrast, cultural psychology goes beyond treating culture as an external variable when it proposes that culture and psyche are mutually constitutive. True psychology of the human experience must encompass world populations through research of the ways in which (a) historically grounded sociocultural contexts enable the distinct meaning systems that people construct, and (b) these systems simultaneously guide the human formation of the environments. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

  15. Cultural Aspects in the Shona Monolingual Dictionary:

    African Journals Online (AJOL)

    Riette Ruthven

    All this knowledge is acquired socially through interaction. ... about the meaning of a word without recognising the wider social and cultural reality of ..... tics. Harmondsworth: Penguin Books. Goodenough, W.H. 1957. Cultural Anthropology and ...

  16. Is «cultural tourism» actually «cultural»?

    Directory of Open Access Journals (Sweden)

    David Senabre López

    2007-09-01

    Full Text Available The epithet of cultural has been used as an adulterated attraction for a kind of leisure activity dressed up in all its finery: the type of tourism that is linked to the historic heritage. The tourism client sets off for an apparent happiness by means of a manipulated, abridged or smoothen by-product of a civilisation’s real historic essence, without paying any attention to –or passively assumin– the mercantilist machinery that it is part of. The label of «cultural» is often distorted by the superficial made of it.Should other forms of tourism that do not involve heritage –such as beach holi- days, rural tourism or mountain holidays– be considered as ‘a-cultural’? Can anybody acquire any culture just by travelling or culture is something that must be carried on by the traveller so as to properly value what he sees? Surveys on cultural tourism so far have concentrated on boosting its efficiency from a business point of view –which is a wrong approach– instead of analysing the phenomenon from a humanistic perspective. Key words: Tourism, Cultura, Leisure, Heritage.

  17. Machinima as a Practice of Meaning-making

    DEFF Research Database (Denmark)

    Frølunde, Lisbeth

    into their stated inspirational resources. The theoretical approach is dialogic communication research and mediated discourse analysis, which aims to uncover the nexus of dynamic creative processes as fundamentally social and polyphonic. Therefore, the study examines the dynamics and significance of the complex...... interactions, relationships, cultural identities, and tools which constitute and sustain machinima activities. This is done by asking questions regarding meaningfulness; what meanings the machinimators ascribe to finished films, how these meanings might have transformed during specific creative processes...

  18. Generation of organotypic raft cultures from primary human keratinocytes.

    Science.gov (United States)

    Anacker, Daniel; Moody, Cary

    2012-02-22

    The development of organotypic epithelial raft cultures has provided researchers with an efficient in vitro system that faithfully recapitulates epithelial differentiation. There are many uses for this system. For instance, the ability to grow three-dimensional organotypic raft cultures of keratinocytes has been an important milestone in the study of human papillomavirus (HPV)(1). The life cycle of HPV is tightly linked to the differentiation of squamous epithelium(2). Organotypic epithelial raft cultures as demonstrated here reproduce the entire papillomavirus life cycle, including virus production(3,4,5). In addition, these raft cultures exhibit dysplastic lesions similar to those observed upon in vivo infection with HPV. Hence this system can also be used to study epithelial cell cancers, as well as the effect of drugs on epithelial cell differentiation in general. Originally developed by Asselineau and Prunieras(6) and modified by Kopan et al.(7), the organotypic epithelial raft culture system has matured into a general, relatively easy culture model, which involves the growth of cells on collagen plugs maintained at an air-liquid interface (Figure 1A). Over the course of 10-14 days, the cells stratify and differentiate, forming a full thickness epithelium that produces differentiation-specific cytokeratins. Harvested rafts can be examined histologically, as well as by standard molecular and biochemical techniques. In this article, we describe a method for the generation of raft cultures from primary human keratinocytes. The same technique can be used with established epithelial cell lines, and can easily be adapted for use with epithelial tissue from normal or diseased biopsies(8). Many viruses target either the cutaneous or mucosal epithelium as part of their replicative life cycle. Over the past several years, the feasibility of using organotypic raft cultures as a method of studying virus-host cell interactions has been shown for several herpesviruses, as

  19. Cultural Considerations: Pharmacological and Nonpharmacological Means for Improving Blood Pressure Control among Hispanic Patients

    Directory of Open Access Journals (Sweden)

    Neela K. Patel

    2012-01-01

    Full Text Available Cardiovascular disease is a leading cause of morbidity and mortality in the United States, and its prevention and treatment remain a priority for the medical community. Ethnic variations account for some differences in the prevalence of hypertension and blood pressure (BP control rates among Hispanics, indicating the need for culturally appropriate management models. Aggressive treatment strategies are key to achieving optimal BP control in high-risk Hispanic patients. Hypertension in this ethnic group continues to be a major health concern. Of note, when provided access to comprehensive care, Hispanics demonstrate similar response rates to treatment as the majority of non-Hispanic whites. This highlights the importance of effective, culturally responsive hypertension management among high-risk Hispanic patients for achieving observable, positive health outcomes.

  20. Symbol/Meaning Paired-Associate Recall: An “Archetypal Memory” Advantage?

    Science.gov (United States)

    Sotirova-Kohli, Milena; Opwis, Klaus; Roesler, Christian; Smith, Steven M.; Rosen, David H.; Vaid, Jyotsna; Djonov, Valentin

    2013-01-01

    The theory of the archetypes and the hypothesis of the collective unconscious are two of the central characteristics of analytical psychology. These provoke, however, varying reactions among academic psychologists. Empirical studies which test these hypotheses are rare. Rosen, Smith, Huston and Gonzales proposed a cognitive psychological experimental paradigm to investigate the nature of archetypes and the collective unconscious as archetypal (evolutionary) memory. In this article we report the results of a cross-cultural replication of Rosen et al. conducted in the German-speaking part of Switzerland. In short, this experiment corroborated previous findings by Rosen et al., based on English speakers, and demonstrated a recall advantage for archetypal symbol meaning pairs vs. other symbol/meaning pairings. The fact that the same pattern of results was observed across two different cultures and languages makes it less likely that they are attributable to a specific cultural or linguistic context. PMID:25379255

  1. 7 CFR 1124.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... section, Producer milk means the skim milk (or skim milk equivalent of components of skim milk), including... filed a request in writing with the market administrator before the first day of the month the agreement... producer deliveries of each according to a method approved by the market administrator. (4) Diverted milk...

  2. The science of culture and negotiation.

    Science.gov (United States)

    Gunia, Brian C; Brett, Jeanne M; Gelfand, Michele J

    2016-04-01

    Recent negotiation research has produced a groundswell of insights about the effects of culture on negotiation. Yet, few frameworks exist to organize the findings. This review integrates recent research using a two-dimensional framework: The first dimension organizes the research into that which has taken: (1) a comparative intracultural approach, versus (2) an intercultural approach. The second dimension organizes the research by its emphasis on: (1) inputs into negotiation, (2) processes of negotiating, and (3) outcomes of negotiation. This framework helps to organize extant research and produces novel insights about the connections between disparate research streams, revealing both commonalities and culture-specificities in negotiation strategy and outcomes and suggesting that intercultural negotiations are difficult but not insurmountable. We conclude by discussing several areas in which more research on culture and negotiation is urgently needed in today's globalizing world. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Birthweight distribution in ART singletons resulting from embryo culture in two different culture media compared with the national population.

    Science.gov (United States)

    Lemmen, J G; Pinborg, A; Rasmussen, S; Ziebe, S

    2014-10-10

    Is there a difference in birthweight distribution in ART singletons born after IVF culture in two different culture media? There is no effect of culture media on both crude and adjusted birthweight distributions in ART singletons from nulliparous mothers. Studies on human ART singletons have reported a difference in birthweight in singletons following IVF culture in different culture media. However, other studies comparing different culture media have not shown any significant differences in birthweight. This study was a retrospective comparison of birthweights in IVF/ICSI singletons conceived after fresh embryo transfer following embryo culture in Cook or Medicult medium and in a national cohort of naturally conceived singletons in nulliparous women. The study compares four independent groups consisting of singletons in nulliparous women from Cook-d2: 2-day culture in Cook medium at Rigshospitalet (n = 974), Medicult-d2: 2-day culture in Medicult EmbryoAssist medium at Rigshospitalet (n = 147), Medicult-d3: 3-day culture in Medicult EmbryoAssist medium with and without added GM-CSF (n = 204), and DK: pregnancies from the Danish birth registry (n = 106842). The study compares the birthweights of singletons from nulliparous women in the four independent groups mentioned above; Cook-d2: Medicult-d2: Medicult-d3: and DK. In addition, distributions of large and small for gestational age infants were compared between the groups and a multiple linear regression analysis was used to determine which factors determined birthweight. We found no significant difference in the crude birthweight distributions between singletons born after culture in Cook-d2 or Medicult-groups. Singleton girls from the Cook-d2 group weighed 3302 ± 28 g, versus 3252 ± 76 in the Medicult-d2 group (difference 50 g; P = 0.547). Singleton boys from the Cook-d2 group weighed 3430 ± 27 g, versus 3354 ± 56 in the Medicult-d2 group (difference 76 g; P = 0.279). In the background population, mean

  4. Linking Shared Meaning to Emergent Literacy: Looking through the Lens of Culture

    Science.gov (United States)

    Howes, Carollee; Wishard, Alison Gallwey

    2004-01-01

    A direct pathway to children's literacy forms through the development of shared meaning. Proto-narrative construction and social pretend play with peers can be important tools in children's developing emergent literacy. Early child-care programs provide relatively little unstructured time. To reemphasize shared meaning in the lives of children,…

  5. Methodology for understanding Indian culture

    DEFF Research Database (Denmark)

    Sinha, Jai; Kumar, Rajesh

    2004-01-01

    Methods of understanding cultures, including Indian culture, are embedded in a broad spectrum of sociocultural approaches to human behavior in general. The approaches examined in this paper reflect evolving perspectives on Indian culture, ranging from the starkly ethnocentric to the largely...... eclectic and integrative. Most of the methods herin discussed were developed in the West and were subsequently taken up with or without adaptations to fit the Indian context. The paper begins by briefly reviewing the intrinsic concept of culture. It then adopts a historical view of the different ways...... and means by which scholars have construed the particular facets of Indian culture, highlighting the advantages and disadvantages of each. The final section concludes with some proposals about the best ways of understnding the complexity that constitutes the Indian cultural reality....

  6. O processo de cuidar da pessoa que sofreu queimaduras: significado cultural atribuído por familiares El proceso de cuidar de personas que sufrieron quemadura: significado cultural atribuido por familiares The process of delivering care to a burned person: cultural meaning attributed by relatives

    Directory of Open Access Journals (Sweden)

    Lídia Aparecida Rossi

    2001-12-01

    Full Text Available Os objetivos deste estudo etnográfico foram identificar o significado cultural atribuído por familiares à participação no processo de cuidar da pessoa ultima de queimadura; identificar as estratégias de cuidado que os familiares focalizam frente à participação no cuidado. Os dados foram coletados mediante entrevistas semi-estruturadas com 25 familiares de clientes que sofreram queimaduras, admitidos em uma Unidade de Queimados e observações participantes. Os resultados mostram que o significado cultural atribuído por familiares de pessoas vítimas queimaduras ao processo de cuidar tem duas dimensões: a física e a moral e gera principalmente medo.Los objetivos fueron investigar los significados culturales atribuidos por familiares a la participación en el proceso de cuidar de personas víctimas de quemadura; identificar las estratégias que los familiares enfocan en su participación en el cuidado. Los datos fueron colectados a través de entrevistas semi-estructuradas con 25 familiares de clientes quemados admitidos en una Unidad de Quemados y a través de observaciones participantes realizadas durante el período de visitas y en reuniones con familiares de clientes. Los resultados de este estudio muestran que la perspectiva de cuidar de una persona que sufrió una quemadura tiene dos dimensiones: la física y la moral, y genera principalmente miedo.This study aimed at investigating the cultural meanings attributed by relatives to their participation in the process of delivering care to burned people; identifying the care strategies focused on by relatives during their participation in the care process. Data were collected by means of semi-structured interviews with 25 relatives of burned patients admitted in a Unit for Burned and by participant observation. The results of this study show that the perspective of giving care to a burned person has two dimensions: a physical dimension and a moral one; and generates mainly fear.

  7. The DaimlerChrysler merger – a cultural mismatch? The DaimlerChrysler merger – a cultural mismatch?

    Directory of Open Access Journals (Sweden)

    Julia Hollmann

    2010-12-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} American company Chrysler Corporation due to differences in the organizational cultures involved or due to a so-called ‘clash of culture'. What happens when two successful car producers with different know-how and a different knowledge background, different work processes, different product portfolios and last but not least, completely different corporate cultures decide to merge? Daimler-Benz and Chrysler wanted to strengthen their position during economically difficult times for the car industry by juggling the crisis together and they hoped to be able to combine their strengths. Therefore the two companies decided to fuse in 1998. But not even ten years later Daimler-Benz once again sold all its shares of the Chrysler division. The dream to become the third biggest car producer of the world, behind General Motors and Ford, burst. The expected and wished for synergy effects stayed out. Instead of gaining competitive advantage over their competitors, the merger rushed the two car producers ever deeper into the crisis and did not provide the companies with the necessary tools to overcome the recession. The presented paper deals with the failed merger of the German company Daimler-Benz with the U.S. American company Chrysler

  8. Isolation of a bacteriocin-producing Lactococcus lactis subsp. lactis and application to control Listeria monocytogenes in Moroccan jben.

    Science.gov (United States)

    Benkerroum, N; Oubel, H; Zahar, M; Dlia, S; Filali-Maltouf, A

    2000-12-01

    Use of a bacteriocin-producing lactococcal strain to control Listeria monocytogenes in jben. A Lactococcus lactis strain isolated from lben was shown, by the spot technique, to produce a bacteriocin different from nisin. Inhibitory activity of the bacteriocin-producing strain against Listeria monocytogenes was investigated in jben, made from cow's milk fermented with the producer organism and contaminated with 104 or 107 cfu ml-1. Listeria counts were monitored during manufacture, and during conservation at room and at refrigeration temperatures. Results showed that the pathogen was reduced by 2.7 logarithmic units after 30 h of jben processing when the initial inoculum of 107 cfu ml(-1) was used. For the initial inoculum of 104 cfu ml(-1), the bacterium was completely eliminated at 24 h. Furthermore, the use of the bacteriocin-producing starter culture extended the shelf-life of jben by 5 days. In situ production of the lactococcal bacteriocin is an efficient biological means of controlling L. monocytogenes in jben and of allowing shelf-life extension. The proposed technology will essentially benefit minimally processed dairy products and those made with raw milk.

  9. Cultural mediation in museums

    Directory of Open Access Journals (Sweden)

    Gherghina Boda

    2017-12-01

    Full Text Available If we perceive the museum not only as a place of storing and conserving the patrimony, but also of transmitting it, then we can also see it as a mediator through which cultures can become collective patrimony. Tightly connected to patrimonial appropriation, mediation appears from this perspective as a process and not an end, as it manifests itself in animation, communication and making knowledge popular in relation to a precise patrimony. That is why we can see cultural mediation as a transmission, as a transformation, as an action or social project which aims at creating social bonds, the museum thus being not only a place of meeting for the public with the objects exposed, but also as a place of meeting between different cultures. Thus, cultural mediation presents itself as the most efficient means for access to culture of all categories of the public, situated as the crossroads of culture, continuous education and entertainment and is inscribed in the field of informal education.

  10. Bile Culture and Susceptibility Testing of Malignant Biliary Obstruction via PTBD

    Energy Technology Data Exchange (ETDEWEB)

    Yu Haipeng; Guo Zhi, E-mail: jieruke@yahoo.com.cn; Xing Wenge; Guo Xiuying; Liu Fang; Li Baoguo [Tinajin Medical University Cancer Institute and Hospital, Department of Interventional Therapy, Tianjin Key Cancer Prevention and Treatment Laboratory (China)

    2012-10-15

    Purpose: To assess the information obtained by bile culture and susceptibility testing for malignant biliary obstruction by a retrospective one-center study. Methods: A total of 694 patients with malignant biliary obstruction received percutaneous transhepatic biliary drainage during the period July 2003 to September 2010, and subsequently, bile specimens were collected during the procedure. Among the 694 patients, 485 were men and 209 were women, ranging in age from 38 to 78 years (mean age 62 years). Results: A total of 42.9% patients had a positive bile culture (298 of 694). Further, 57 species of microorganisms and 342 strains were identified; gram-positive bacteria accounted for 50.9% (174 of 342) and gram-negative bacteria accounted for 41.5% (142 of 342) of these strains. No anaerobes were obtained by culture during this study. The most common microorganisms were Enterococcus faecalis (41 of 342, 11.9%), Escherichia coli (34 of 342, 9.9%), Klebsiella pneumoniae (28 of 342, 8.2%), Staphylococcus epidermidis (19 of 342, 5.5%), Enterococcus (18 of 342, 5.3%), and Enterobacter cloacae (16 of 342, 4.7%). The percentage of {beta}-lactamase-producing gram-positive bacteria was 27.6% (48 of 174), and the percentage of gram-negative bacteria was 19.7% (28 of 142). The percentage of enzyme-producing Escherichia coli was 61.7% (21 of 34). Conclusion: The bile cultures in malignant biliary obstruction are different from those in the Tokyo Guidelines and other benign biliary obstruction researches, which indicates that a different antibacterial therapy should be applied. Thus, knowledge of the antimicrobial susceptibility data could aid in the better use of antibiotics for the empirical therapy of biliary infection combined with malignant biliary obstruction.

  11. Bile Culture and Susceptibility Testing of Malignant Biliary Obstruction via PTBD

    International Nuclear Information System (INIS)

    Yu Haipeng; Guo Zhi; Xing Wenge; Guo Xiuying; Liu Fang; Li Baoguo

    2012-01-01

    Purpose: To assess the information obtained by bile culture and susceptibility testing for malignant biliary obstruction by a retrospective one-center study. Methods: A total of 694 patients with malignant biliary obstruction received percutaneous transhepatic biliary drainage during the period July 2003 to September 2010, and subsequently, bile specimens were collected during the procedure. Among the 694 patients, 485 were men and 209 were women, ranging in age from 38 to 78 years (mean age 62 years). Results: A total of 42.9% patients had a positive bile culture (298 of 694). Further, 57 species of microorganisms and 342 strains were identified; gram-positive bacteria accounted for 50.9% (174 of 342) and gram-negative bacteria accounted for 41.5% (142 of 342) of these strains. No anaerobes were obtained by culture during this study. The most common microorganisms were Enterococcus faecalis (41 of 342, 11.9%), Escherichia coli (34 of 342, 9.9%), Klebsiella pneumoniae (28 of 342, 8.2%), Staphylococcus epidermidis (19 of 342, 5.5%), Enterococcus (18 of 342, 5.3%), and Enterobacter cloacae (16 of 342, 4.7%). The percentage of β-lactamase-producing gram-positive bacteria was 27.6% (48 of 174), and the percentage of gram-negative bacteria was 19.7% (28 of 142). The percentage of enzyme-producing Escherichia coli was 61.7% (21 of 34). Conclusion: The bile cultures in malignant biliary obstruction are different from those in the Tokyo Guidelines and other benign biliary obstruction researches, which indicates that a different antibacterial therapy should be applied. Thus, knowledge of the antimicrobial susceptibility data could aid in the better use of antibiotics for the empirical therapy of biliary infection combined with malignant biliary obstruction.

  12. Utilization of bacteriocin-producing bacteria in dairy products

    Directory of Open Access Journals (Sweden)

    Matěj Patrovský

    2016-07-01

    Full Text Available Lactic acid bacteria have been used since ancient times for food preparation and for bio-conservation by fermentation. Selected strains are capable of producing antimicrobial peptides - bacteriocins, which can be natural preservatives, especially in products with short shelf lives. The present study is focused on inhibitory effects of the bacteriocin-producing bacteria strains Enterococcus faecium, Pediococccus acidilactici and Lactobacillus plantarum against Listeria innocua as an indicator microorganism. Freeze-dried preparations of bacterial strains producing particular bacteriocins were tested by agar well-diffusion assay and by the traditional spread plate method. Plantaricin exhibited the highest anti-listerial effect among the tested bacteriocins. Pediocin also demonstrated a distinct inhibitory effect, but enterocin appeared to be heat labile and its efficiency was also suppressed under cold storage conditions. Plantaricin reduced Listeria innocua counts by 1 log in dairy spread made from cheese and quark. The formation of bacteriocins by various Lactobacillus plantarum strains were substantially influenced by the cultivation conditions of the mother culture and by the microbial preparation process before freeze-drying. Bacteriocins introduced into foodstuffs via protective cultures in situ offer new perspectives on enhancing food quality and safety.

  13. Isolation of TDA-producing Phaeobacter strains from sea bass larval rearing units and their probiotic effect against pathogenic Vibrio spp. in Artemia cultures

    DEFF Research Database (Denmark)

    Grotkjær, Torben; Bentzon-Tilia, Mikkel; D'Alvise, Paul

    2016-01-01

    V. harveyi, which is the major bacterial pathogen in crustaceans and Mediterranean sea bass larvae cultures. Concomitantly, they significantly improved survival of V. harveyi-infected brine shrimp. 16S rRNA gene sequence homology identified the antagonists as Phaeobacter sp., and in silico DNA...... Vibrio anguillarum and reduce mortality in V. anguillarum-infected cod and turbot larvae. In this study, it was demonstrated that antagonistic Roseobacter-clade bacteria could be isolated from sea bass larval rearing units. In addition, it was shown that they not only antagonized V. anguillarum but also......-producing Phaeobacter isolated from Mediterranean marine larviculture are promising probiotic bacteria against pathogenic Vibrio in crustacean live-feed cultures for marine fish larvae....

  14. Concept analysis of culture applied to nursing.

    Science.gov (United States)

    Marzilli, Colleen

    2014-01-01

    Culture is an important concept, especially when applied to nursing. A concept analysis of culture is essential to understanding the meaning of the word. This article applies Rodgers' (2000) concept analysis template and provides a definition of the word culture as it applies to nursing practice. This article supplies examples of the concept of culture to aid the reader in understanding its application to nursing and includes a case study demonstrating components of culture that must be respected and included when providing health care.

  15. Hydroponics or soilless culture

    Science.gov (United States)

    Chapman, H. D.

    1963-01-01

    Historically, hydroponics is not a new field; plant physiologists have known and used it for some 100 years. Inevitably, some enthusiasts got carried away.Claims were made of enormous potential yields; skyscraper tops were said to be capable of producing enough food for all of their occupants; and closets, basements, garages, etc. were wishfully converted into fields for hydroponic culture. Numerous publications on the subject appeared during this period. Basic requirements for hydropinc techniques are given along with examples of where soilless culture has been used commercially.

  16. The Cultured Word: Cultural Background, Bilingualism, and the School Library.

    Science.gov (United States)

    Agosto, Denise E.

    2001-01-01

    Presents major research related to cultural background as a framework for textual meaning-making, bilingualism, and literacy development. Discusses bilingualism, literacy, and social context; considers why these issues are important to school librarians; and offers suggestions for making multicultural materials central aspects of school library…

  17. Raman spectroscopy analysis of differences in composition of spent culture media of in vitro cultured preimplantation embryos isolated from normal and fat mice dams.

    Science.gov (United States)

    Fabian, Dušan; Kačmarová, Martina; Kubandová, Janka; Čikoš, Štefan; Koppel, Juraj

    2016-06-01

    The aim of the present study was to compare overall patterns of metabolic activity of in vitro cultured preimplantation embryos isolated from normal and fat mice dams by means of non-invasive profiling of spent culture media using Raman spectroscopy. To produce females with two different types of body condition (normal and fat), a previously established two-generation model was used, based on overfeeding of experimental mice during prenatal and early postnatal development. Embryos were isolated from spontaneously ovulating and naturally fertilized dams at the 2-cell stage of development and cultured to the blastocyst stage in synthetic oviductal medium KSOMaa. Embryos from fat mice (displaying significantly elevated body weight and fat) showed similar developmental capabilities in vitro as embryos isolated from normal control dams (displaying physiological body weight and fat). The results show that alterations in the composition of culture medium caused by the presence of developing mouse preimplantation embryos can be detected using Raman spectroscopy. Metabolic activity of embryos was reflected in evident changes in numerous band intensities in the 1620-1690cm(-1) (amide I) region and in the 1020-1140cm(-1) region of the Raman spectrum for KSOMaa. Moreover, multivariate analysis of spectral data proved that the composition of proteins and other organic compounds in spent samples obtained after the culture of embryos isolated from fat dams was different from that in spent samples obtained after the culture of embryos from control dams. This study demonstrates that metabolic activity of cultured preimplantation embryos might depend on the body condition of their donors. Copyright © 2016 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  18. Media and Cultural Industries Internships: A Thematic Review and Digital Labor Parallels

    Directory of Open Access Journals (Sweden)

    Thomas Corrigan

    2015-09-01

    Full Text Available This article reviews existing research on the motivations and experiences of interns in media and cultural industries. Digital labour theories are used to organize and make sense of the existing internship literature. Throughout the article, parallels are also drawn between the experiences of interns and those of digital creative labourers—both professionals and peer producers. Three key themes are identified within the internship literature: 1 interns derive satisfaction from work they con- sider meaningful, particularly hands-on work executed under the training and trust of effective supervisors; 2 interns see their work as future-oriented investments in their skills, professional networks, and personal brands; and 3 the ambiguity and professional necessity of media and cultural industries internships make them fertile ground for exploitation and self-exploitation. In conclusion, I argue that attentiveness to meaning, temporality, and ambiguity will be essential to future critical investigations of internships.

  19. Political Culture and Covalent Bonding. A Conceptual Model of Political Culture Change

    OpenAIRE

    Camelia Florela Voinea

    2015-01-01

    Our class of models aims at explaining the dynamics of political attitude change by means of the dynamic changes in values, beliefs, norms and knowledge with which it is associated. The model constructs a political culture perspective over the relationship between macro and micro levels of a society and polity. The model defines the bonding mechanism as a basic mechanism of the political culture change by taking inspiration from the valence bonding theory in Chemistry, which has inspired the ...

  20. Fatty acid-producing hosts

    Science.gov (United States)

    Pfleger, Brian F; Lennen, Rebecca M

    2013-12-31

    Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally delected. The hosts prefereably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recominantly stable and growth-competent at 37.degree. C. Methods of producing a fatty acid product comprising culturing such hosts at 37.degree. C. are also described.

  1. Culture: copying, compression, and conventionality.

    Science.gov (United States)

    Tamariz, Mónica; Kirby, Simon

    2015-01-01

    Through cultural transmission, repeated learning by new individuals transforms cultural information, which tends to become increasingly compressible (Kirby, Cornish, & Smith, ; Smith, Tamariz, & Kirby, ). Existing diffusion chain studies include in their design two processes that could be responsible for this tendency: learning (storing patterns in memory) and reproducing (producing the patterns again). This paper manipulates the presence of learning in a simple iterated drawing design experiment. We find that learning seems to be the causal factor behind the increase in compressibility observed in the transmitted information, while reproducing is a source of random heritable innovations. Only a theory invoking these two aspects of cultural learning will be able to explain human culture's fundamental balance between stability and innovation. Copyright © 2014 Cognitive Science Society, Inc.

  2. The environmental prospects of cultured meat in China

    Institute of Scientific and Technical Information of China (English)

    SUN Zhi-chang; YU Qun-li; HAN Lin

    2015-01-01

    To deal with concerns in China about environmental degradation and a growth in population accompanied by increased consumption of livestock products, a meat alternative is required. This study compared the environmental impacts of producing different protein sources for nutrition, including crops, livestock products, and cultured meat. The results showed that cultured meat has the lowest land use per unit of protein and unit of human digestible energy. China’s crops have the lowest energy use and greenhouse gas (GHG) emissions per unit of energy and protein. The energy use in cultured meat production is slightly higher than that of current pork production in China, whereas GHG emissions are lower. It is concluded that the overal impact of replacing livestock products with cultured meat would be beneifcial for China’s environment and would potential y improve food security because less land is needed to produce the same amount of protein and energy.

  3. Bioleaching of metals from printed circuit boards supported with surfactant-producing bacteria

    International Nuclear Information System (INIS)

    Karwowska, Ewa; Andrzejewska-Morzuch, Dorota; Łebkowska, Maria; Tabernacka, Agnieszka; Wojtkowska, Małgorzata; Telepko, Alicja; Konarzewska, Agnieszka

    2014-01-01

    Highlights: • Bioleaching of metals from printed circuit boards by BSAC-producing bacteria was estimated. • Aeration increased the release of all metals in medium with sulphur and biosurfactant. • Increase in Cu, Pb, Ni and Cr removal rate was observed at 37 °C in acidic medium. -- Abstract: This study has evaluated the possibility of bioleaching zinc, copper, lead, nickel, cadmium and chromium from printed circuit boards by applying a culture of sulphur-oxidising bacteria and a mixed culture of biosurfactant-producing bacteria and sulphur-oxidising bacteria. It was revealed that zinc was removed effectively both in a traditional solution acidified by a way of microbial oxidation of sulphur and when using a microbial culture containing sulphur-oxidising and biosurfactant-producing bacteria. The average process efficiency was 48% for Zn dissolution. Cadmium removal was similar in both media, with a highest metal release of 93%. For nickel and copper, a better effect was obtained in the acidic medium, with a process effectiveness of 48.5% and 53%, respectively. Chromium was the only metal that was removed more effectively in the bioleaching medium containing both sulphur-oxidising and biosurfactant-producing bacteria. Lead was removed from the printed circuit boards with very low effectiveness (below 0.5%). Aerating the culture medium with compressed air increased the release of all metals in the medium with sulphur and biosurfactant, and of Ni, Cu, Zn and Cr in the acidic medium. Increasing the temperature of the medium (to 37 °C) had a more significant impact in the acidic environment than in the neutral environment

  4. Bioleaching of metals from printed circuit boards supported with surfactant-producing bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Karwowska, Ewa, E-mail: ewa.karwowska@is.pw.edu.pl [Warsaw University of Technology, Faculty of Environmental Engineering, Biology Division, Nowowiejska 20, 00-653 Warsaw (Poland); Andrzejewska-Morzuch, Dorota; Łebkowska, Maria [Warsaw University of Technology, Faculty of Environmental Engineering, Biology Division, Nowowiejska 20, 00-653 Warsaw (Poland); Tabernacka, Agnieszka, E-mail: agnieszka.tabernacka@is.pw.edu.pl [Warsaw University of Technology, Faculty of Environmental Engineering, Biology Division, Nowowiejska 20, 00-653 Warsaw (Poland); Wojtkowska, Małgorzata; Telepko, Alicja; Konarzewska, Agnieszka [Warsaw University of Technology, Faculty of Environmental Engineering, Nowowiejska 20, 00-653 Warsaw (Poland)

    2014-01-15

    Highlights: • Bioleaching of metals from printed circuit boards by BSAC-producing bacteria was estimated. • Aeration increased the release of all metals in medium with sulphur and biosurfactant. • Increase in Cu, Pb, Ni and Cr removal rate was observed at 37 °C in acidic medium. -- Abstract: This study has evaluated the possibility of bioleaching zinc, copper, lead, nickel, cadmium and chromium from printed circuit boards by applying a culture of sulphur-oxidising bacteria and a mixed culture of biosurfactant-producing bacteria and sulphur-oxidising bacteria. It was revealed that zinc was removed effectively both in a traditional solution acidified by a way of microbial oxidation of sulphur and when using a microbial culture containing sulphur-oxidising and biosurfactant-producing bacteria. The average process efficiency was 48% for Zn dissolution. Cadmium removal was similar in both media, with a highest metal release of 93%. For nickel and copper, a better effect was obtained in the acidic medium, with a process effectiveness of 48.5% and 53%, respectively. Chromium was the only metal that was removed more effectively in the bioleaching medium containing both sulphur-oxidising and biosurfactant-producing bacteria. Lead was removed from the printed circuit boards with very low effectiveness (below 0.5%). Aerating the culture medium with compressed air increased the release of all metals in the medium with sulphur and biosurfactant, and of Ni, Cu, Zn and Cr in the acidic medium. Increasing the temperature of the medium (to 37 °C) had a more significant impact in the acidic environment than in the neutral environment.

  5. Culture and its neurofunctional correlates when death is in mind.

    Science.gov (United States)

    Graupmann, Verena; Peres, Isabella; Michaely, Tonia; Meindl, Thomas; Frey, Dieter; Reiser, Maximilian; Pöppel, Ernst; Fehse, Kai; Gutyrchik, Evgeny

    2013-08-26

    The human fear of death is marked by specific psychological reactions that affirm cultural belonging. Terror management theory explains this phenomenon with the symbolic immortality provided by collective meaning in culture. This coping has also been explained with the motive of maintaining a meaningful representation of the world. Here we show that neural patterns of activations corresponding to cultural worldview defense processes differed when images that affirmed participants' cultural heritage were preceded by death-related verbal primes versus verbal primes threatening meaning. Cultural content was drawn upon distinctly on a neural basis when facing death-related cognitions. The neural representation of cultural coping sheds light on the immediate mechanisms in compensating the human fear of death. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  6. The Role of Food Selection in Swedish Home Economics: The Educational Visions and Cultural Meaning.

    Science.gov (United States)

    Höijer, Karin; Hjälmeskog, Karin; Fjellström, Christina

    2014-01-01

    This article explores foods talked about and chosen in the education of Swedish Home Economics as a relationship between structural processes and agency. Three data sets from observations and focus group interviews with teachers and students were analyzed for food classifications. These were related to a culinary triangle of contradictions, showing factors of identity, convenience and responsibility. Results show that foods talked about and chosen by teachers and students were reflections of dominant cultural values. Results also indicate that teachers had more agency than students, but that the choices they made were framed by educational visions and cultural values.

  7. Investing in organisational culture: nursing students' experience of organisational learning culture in aged care settings following a program of cultural development.

    Science.gov (United States)

    Grealish, Laurie; Henderson, Amanda

    2016-10-01

    Concerns around organisational learning culture limit nursing student placements in aged care settings to first year experiences. Determine the impact of an extended staff capacity building program on students' experiences of the organisational learning culture in the aged care setting. Pre and post-test design. A convenience sample of first, second and third year Bachelor of Nursing students attending placements at three residential aged care facilities completed the Clinical Learning Organisational Culture Survey. Responses between the group that attended placement before the program (n = 17/44; RR 38%) and the group that attended following the program (n = 33/72; RR 45%) were compared. Improvements were noted in the areas of recognition, accomplishment, and influence, with decreases in dissatisfaction. Organisational investment in building staff capacity can produce a positive learning culture. The aged care sector offers a rich learning experience for students when staff capacity to support learning is developed.

  8. Tomboys: meanings, marginalization, and misunderstandings.

    Science.gov (United States)

    Hall, Joanne M

    2008-06-01

    Girls who act like boys are called "tomboys." This is an exploration using feminist guiding principles, about what "tomboyism" means to children and how adults around them construct it. The purpose is to examine tomboyism from literary, linguistic, historical and cultural perspectives. The exploration includes a review of selected scientific sources, children's and some adult literature, and conversations with adult women who recall their tomboyism from youth and how it now affects them as adults.

  9. Development of three-dimensional cellular culture system for testing of biological effects of radiations in tumoral and non-tumoral models

    International Nuclear Information System (INIS)

    Bonfim, Leticia; Vieira, Daniel Perez; Oliveira, Karina

    2017-01-01

    Pre-clinical drug testing is currently based on based on monolayer or 2D (2D) cell cultures and, despite the large-scale use of this form of culture, there is already scientific evidence that the cellular disposition in monolayers does not adequately simulate tissue physiology, as it prevents cells from expressing their characteristics in a manner analogous to that found in the organism. For this purpose, the work aimed to produce three-dimensional structures, referred as spheroids, using magnetic levitation by adding iron nanoparticles to the cultures and with the aid of magnets. Electron microscopy showed particles with about 20nm in diameter. FTIR (Fourier-transform infrared spectroscopy) analysis showed stretches compatible with iron and amino acid (Lysine) binding. The images showed the formation of spherical bodies until the ninth day. LnCap spheroid diameter varied from (mean ± error) 434.407 ± 50.018 μm (5 th day) to 264.574 ± 13.184 μm (9 t 'h day). Cultures of CHO ranged from 229.237 ± 5.278 μm to 236.719 ± 12.910 μm in the same period. Spheres generated by magnetic levitation could be measured by digital means and compared throughout the experiment. The tool can be used to test the biological effects of radiation and / or radiopharmaceuticals in culture. (author)

  10. Development of three-dimensional cellular culture system for testing of biological effects of radiations in tumoral and non-tumoral models

    Energy Technology Data Exchange (ETDEWEB)

    Bonfim, Leticia; Vieira, Daniel Perez, E-mail: leticia.bonfim@ipen.br [Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP), São Paulo, SP (Brazil). Lab. de Radiobiologia; Oliveira, Karina [Universidade Federal de Sao Paulo (UNIFESP), Diadema, SP (Brazil). Departamento de Fisica

    2017-07-01

    Pre-clinical drug testing is currently based on based on monolayer or 2D (2D) cell cultures and, despite the large-scale use of this form of culture, there is already scientific evidence that the cellular disposition in monolayers does not adequately simulate tissue physiology, as it prevents cells from expressing their characteristics in a manner analogous to that found in the organism. For this purpose, the work aimed to produce three-dimensional structures, referred as spheroids, using magnetic levitation by adding iron nanoparticles to the cultures and with the aid of magnets. Electron microscopy showed particles with about 20nm in diameter. FTIR (Fourier-transform infrared spectroscopy) analysis showed stretches compatible with iron and amino acid (Lysine) binding. The images showed the formation of spherical bodies until the ninth day. LnCap spheroid diameter varied from (mean ± error) 434.407 ± 50.018 μm (5{sup th} day) to 264.574 ± 13.184 μm (9{sup t}'h day). Cultures of CHO ranged from 229.237 ± 5.278 μm to 236.719 ± 12.910 μm in the same period. Spheres generated by magnetic levitation could be measured by digital means and compared throughout the experiment. The tool can be used to test the biological effects of radiation and / or radiopharmaceuticals in culture. (author)

  11. Control system for a nuclear power producing unit

    International Nuclear Information System (INIS)

    Durrant, O.W.

    1978-01-01

    The invention provides in a control system for a nuclear power producing unit comprising a pressurized water reactor, a once-through steam generator provided with feedwater supply means, a turbine-generator supplied with steam from the steam generator and means maintaining a flow of pressurized water through the reactor and steam generator. The combination comprising; means generating a feed forward control signal proportional to the desired power output of the power producing unit, a second means for adjusting the reactor heat release, a third means for adjusting the rate of flow of feedwater to the steam generator, the second and third means solely responsive to and operated in parallel from the feed forward control signal whereby the reactor heat release and the rate of flow of feedwater to the steam generator are each maintained in a discrete functional relationship to the feed forward control signal

  12. Care meanings, expressions, and experiences of those with chronic mental illness.

    Science.gov (United States)

    George, Tamara B

    2002-02-01

    The care meanings, expressions, and experiences of those with a chronic mental illness living in the community were explored with use of Leininger's Theory of Culture Care Diversity and Universality and the Sunrise Model. Results indicate that people with chronic mental illness have identifiable values, norms, and lifeways that set them apart from the dominant culture. Cultural and social structure factors, ethnohistory, and environmental context influence their desired care. Nurses can use this knowledge to provide culturally congruent care in new ways to enhance the quality of life, productivity, and well-being of this subculture. Copyright 2002 by W.B. Saunders Company

  13. EDUCATIONAL OPPORTUNITIES OF COLLAGE AS A DIDACTIC MEANS: INTERDISCIPLINARY APPROACH

    Directory of Open Access Journals (Sweden)

    I. B. Ryzhkina

    2014-01-01

    Full Text Available The research aim is to examine the functions and educational opportunities of collage as a didactic means.The methodology and methods involve the multilateral theoretical data analysis and generalization; activity approach to pupils’ perception analysis; communicative approach to finding the interrelation between the perception and speech act; lingua-cultural approach to collage consideration as a sign, information vehicle and communication subject; and developmental teaching approach.The author undertakes the cross-disciplinary analysis, including the scientific works on philosophy, psychology, semiotics and culture studies, and proves the collage adequacy to the cognitive specificity of modern pupils’perception and foreign-language teaching. The concept of collage is specified, its semiotic characteristics and unique features as a didactic means discussed.The research novelty involves developing a new frame of reference to collage as a didactic tool and cultural phenomenon with educational and developmental opportunities.The research findings, including the methodology basis for content structuring and selecting a collage type, can be used for educational problem solving.

  14. The discriminant validity of the culture assessment instrument: A comparison of company sub-cultures

    Directory of Open Access Journals (Sweden)

    Leo Petkoon

    2004-10-01

    Full Text Available The primary purpose of this study was to assess the discriminant validity of the Culture Assessment Instrument (CAI; that is to distinguish between company mean sub-culture scores and between mean scores of a target company and that of a norm group. The primary data was obtained by a sample of convenience (N = 593 from a transport organisation. The secondary data of the norm group was constituted by convenience samples (N = 4066 from various companies originating from different industries. The 56 item scores of the CAI were factor analysed on two levels followed by iterative item analyses. Although significant differences were detected between mean culture scores, only a small proportion of the variance in these scores could be attributed to culture differences. On these grounds, the CAI does not possess discriminant validity. Suggestions for improving the CAI were made. Opsomming Die primêre doel van die studie was om die diskriminante geldigheid van die ‘Culture Assessment Instrument’ (CAI te beoordeel; dit is om tussen ondernemings se gemiddelde kultuur-subtelling te onderskei en tussen die gemiddelde tellings van ‘n teiken onderneming en ’n normgroep. Die primêre data is verkry van ’n geleentheidsteekproef (N = 593 uit ’n transport-onderneming. Die sekondêre data van die normgroep is saamgestel uit geleentheidsteekproewe (N = 4066 van verskillende ondernemings afkomstig uit verskeie industrieë. Die 56 itemtellings van die CAI is op twee vlakke gefaktoranaliseer, gevolg deur iteratiewe itemontledings. Ofskoon beduidende verskille tussen gemiddelde kultuurtellings gevind is, kon slegs ’n klein proporsie van die variansie in die tellings aan kultuurverskille toegeskryf word. Op hierdie gronde beskik die CAI nie oor diskriminante geldigheid nie. Voorstelle ter verbetering van die CAI is gemaak.

  15. Mean Profiles of the NEO Personality Inventory

    Czech Academy of Sciences Publication Activity Database

    Allik, J.; Church, A.T.; Ortiz, F. A.; Rossier, J.; Hřebíčková, Martina; De Fruyt, F.; Realo, A.; McCrae, R. R.

    2017-01-01

    Roč. 48, č. 3 (2017), s. 402-420 ISSN 0022-0221 Institutional support: RVO:68081740 Keywords : personality * five-factor model * cross-cultural research * traits * country mean scores * NEO-PI-R * NEO-PI-3 Subject RIV: AN - Psychology OBOR OECD: Psychology (including human - machine relations) Impact factor: 1.657, year: 2016

  16. Use of the α-mannosidase I inhibitor kifunensine allows the crystallization of apo CTLA-4 homodimer produced in long-term cultures of Chinese hamster ovary cells

    International Nuclear Information System (INIS)

    Yu, Chao; Crispin, Max; Sonnen, Andreas F.-P.; Harvey, David J.; Chang, Veronica T.; Evans, Edward J.; Scanlan, Christopher N.; Stuart, David I.; Gilbert, Robert J. C.; Davis, Simon J.

    2011-01-01

    The α-mannosidase I inhibitor kifunensine inhibited N-glycan processing in long-term cultures of Chinese hamster ovary cells, allowing deglycosylation and crystallization of the homodimeric extracellular region of the inhibitory glycoprotein receptor CTLA-4 (CD152). Glycoproteins present problems for structural analysis since they often have to be glycosylated in order to fold correctly and because their chemical and conformational heterogeneity generally inhibits crystallization. It is shown that the α-mannosidase I inhibitor kifunensine, which has previously been used for the purpose of glycoprotein crystallization in short-term (3–5 d) cultures, is apparently stable enough to be used to produce highly endoglycosidase H-sensitive glycoprotein in long-term (3–4 week) cultures of stably transfected Chinese hamster ovary (CHO) cells. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based analysis of the extracellular region of the cytotoxic T-lymphocyte antigen 4 (CTLA-4; CD152) homodimer expressed in long-term CHO cell cultures in the presence of kifunensine revealed that the inhibitor restricted CTLA-4 glycan processing to Man 9 GlcNAc 2 and Man 5 GlcNAc 2 structures. Complex-type glycans were undetectable, suggesting that the inhibitor was active for the entire duration of the cultures. Endoglycosidase treatment of the homodimer yielded protein that readily formed orthorhombic crystals with unit-cell parameters a = 43.9, b = 51.5, c = 102.9 Å and space group P2 1 2 1 2 1 that diffracted to Bragg spacings of 1.8 Å. The results indicate that kifunensine will be effective in most, if not all, transient and long-term mammalian cell-based expression systems

  17. Framing financial culture

    DEFF Research Database (Denmark)

    Just, Sine Nørholm; Mouton, Nicolaas T.O.

    2014-01-01

    between competing frames leads to the conclusion that this political “blame game” is related to struggles over how to define the scandal, how to conceptualize its causes, and policy recommendations. Banks may have lost the battle of “Liborgate,” but the war over the meaning of financial culture is far...... from over. Originality/value – The paper is theoretically and methodologically original in its combination of the theories of framing and stasis, and it provides analytical insights into how sense is made of financial culture in the wake of the financial crisis....

  18. Patient Safety Culture

    DEFF Research Database (Denmark)

    Kristensen, Solvejg

    of health care professional’s behaviour, habits, norms, values, and basic assumptions related to patient care; it is the way things are done. The patient safety culture guides the motivation, commitment to and know-how of the safety management, and how all members of a work place interact. This thesis......Patient safety is highly prioritised in the Danish health care system, never the less, patients are still exposed to risk and harmed every day. Implementation of a patient safety culture has been suggested an effective mean to protect patients against adverse events. Working strategically...

  19. Rudimentary, low tech incinerators as a means to produce reactive pozzolan out of sugar cane straw

    International Nuclear Information System (INIS)

    Martirena, Fernando; Middendorf, Bernhard; Day, Robert L.; Gehrke, Matthias; Roque, Pablo; Martinez, Lesday; Betancourt, Sergio

    2006-01-01

    The ashes of agricultural wastes from the processing of sugar cane are recognized as having pozzolanic properties. Burning of these wastes under controlled conditions, e.g. temperature and residence time results in significant improvement in reactivity. There are many reports of low-tech incinerators that have been successfully used to produce reactive rice husk ash in Asia. The paper presents the results of the evaluation of a rudimentary incinerator where sugar cane straw is burnt in order to obtain a reactive ash. The incinerator is designed and constructed according to state-of-the-art recommendations for this kind of device. Various burning trials were performed in order to obtain ash for the experiment. X-ray diffraction analysis performed on powdered ash shows significant presence of amorphous (glassy) material. Lime-pozzolana pastes were prepared. The pastes were subjected to X-ray diffraction, thermo-gravimetric analysis, chemical titration, and SEM observation, as a means to examine the pozzolanicity of the ash via the progress with time of calcium hydroxide consumption, and changes in the pore size distribution and strength. Calcium silicate hydrate phases are the main reaction product of the pozzolanic reaction. The long residence time of the ash in the burning chamber seems to be the reason for the fairly low reactivity of the ash; the reactivity of the ash was not significantly improved in comparison with that of the ash burnt in uncontrolled conditions in the open air

  20. Developing a mesophilic co-culture for direct conversion of cellulose to butanol in consolidated bioprocess.

    Science.gov (United States)

    Wang, Zhenyu; Cao, Guangli; Zheng, Ju; Fu, Defeng; Song, Jinzhu; Zhang, Junzheng; Zhao, Lei; Yang, Qian

    2015-01-01

    Consolidated bioprocessing (CBP) of butanol production from cellulosic biomass is a promising strategy for cost saving compared to other processes featuring dedicated cellulase production. CBP requires microbial strains capable of hydrolyzing biomass with enzymes produced on its own with high rate and high conversion and simultaneously produce a desired product at high yield. However, current reported butanol-producing candidates are unable to utilize cellulose as a sole carbon source and energy source. Consequently, developing a co-culture system using different microorganisms by taking advantage of their specific metabolic capacities to produce butanol directly from cellulose in consolidated bioprocess is of great interest. This study was mainly undertaken to find complementary organisms to the butanol producer that allow simultaneous saccharification and fermentation of cellulose to butanol in their co-culture under mesophilic condition. Accordingly, a highly efficient and stable consortium N3 on cellulose degradation was first developed by multiple subcultures. Subsequently, the functional microorganisms with 16S rRNA sequences identical to the denaturing gradient gel electrophoresis (DGGE) profile were isolated from consortium N3. The isolate Clostridium celevecrescens N3-2 exhibited higher cellulose-degrading capability was thus chosen as the partner strain for butanol production with Clostridium acetobutylicum ATCC824. Meanwhile, the established stable consortium N3 was also investigated to produce butanol by co-culturing with C. acetobutylicum ATCC824. Butanol was produced from cellulose when C. acetobutylicum ATCC824 was co-cultured with either consortium N3 or C. celevecrescens N3-2. Co-culturing C. acetobutylicum ATCC824 with the stable consortium N3 resulted in a relatively higher butanol concentration, 3.73 g/L, and higher production yield, 0.145 g/g of glucose equivalent. The newly isolated microbial consortium N3 and strain C. celevecrescens N3

  1. Recombinant human IGF-1 produced by transgenic plant cell suspension culture enhances new bone formation in calvarial defects.

    Science.gov (United States)

    Poudel, Sher Bahadur; Bhattarai, Govinda; Kook, Sung-Ho; Shin, Yun-Ji; Kwon, Tae-Ho; Lee, Seung-Youp; Lee, Jeong-Chae

    2017-10-01

    Transgenic plant cell suspension culture systems have been utilized extensively as convenient and efficient expression systems for the production of recombinant human growth factors. We produced insulin-like growth factor-1 using a plant suspension culture system (p-IGF-1) and explored its effect on new bone formation in calvarial defects. We also compared the bone regenerating potential of p-IGF-1 with commercial IGF-1 derived from Escherichia coli (e-IGF-1). Male C57BL/6 mice underwent calvarial defect surgery, and the defects were loaded with absorbable collagen sponge (ACS) only (ACS group) or ACS impregnated with 13μg of p-IGF-1 (p-IGF-1 group) or e-IGF-1 (e-IGF-1 group). The sham group did not receive any treatment with ACS or IGFs after surgery. Live μCT and histological analyses showed critical-sized bone defects in the sham group, whereas greater bone formation was observed in the p-IGF-1 and e-IGF-1 groups than the ACS group both 5 and 10weeks after surgery. Bone mineral density, bone volume, and bone surface values were also higher in the IGF groups than in the ACS group. Local delivery of p-IGF-1 or e-IGF-1 more greatly enhanced the expression of osteoblast-specific markers, but inhibited osteoclast formation, in newly formed bone compared with ACS control group. Specifically, p-IGF-1 treatment induced higher expression of alkaline phosphatase, osteocalcin, and osteopontin in the defect site than did e-IGF-1. Furthermore, treatment with p-IGF-1, but not e-IGF-1, increased mineralization of MC3T3-E1 cells, with the attendant upregulation of osteogenic marker genes. Collectively, our findings suggest the potential of p-IGF-1 in promoting the processes required for bone regeneration. Copyright © 2017. Published by Elsevier Ltd.

  2. Characterization of multiple antilisterial peptides produced by sakacin P-producing Lactobacillus sakei subsp. sakei 2a.

    Science.gov (United States)

    Carvalho, Kátia G; Bambirra, Felipe H S; Nicoli, Jacques R; Oliveira, Jamil S; Santos, Alexandre M C; Bemquerer, Marcelo P; Miranda, Antonio; Franco, Bernadette D G M

    2018-05-01

    Antimicrobial compounds produced by lactic acid bacteria can be explored as natural food biopreservatives. In a previous report, the main antimicrobial compounds produced by the Brazilian meat isolate Lactobacillus sakei subsp. sakei 2a, i.e., bacteriocin sakacin P and two ribosomal peptides (P2 and P3) active against Listeria monocytogenes, were described. In this study, we report the spectrum of activity, molecular mass, structural identity and mechanism of action of additional six antilisterial peptides produced by Lb. sakei 2a, detected in a 24 h-culture in MRS broth submitted to acid treatment (pH 1.5) and proper fractionation and purification steps for obtention of free and cell-bound proteins. The six peptides presented similarity to different ribosomal proteins of Lb. sakei subsp sakei 23K and the molecular masses varied from 4.6 to 11.0 kDa. All peptides were capable to increase the efflux of ATP and decrease the membrane potential in Listeria monocytogenes. The activity of a pool of the obtained antilisterial compounds [enriched active fraction (EAF)] against Listeria monocytogenes in a food model (meat gravy) during refrigerated storage (4 °C) for 10 days was also tested and results indicated that the populations of L. monocytogenes in the food model containing the acid extract remained lower than those at time 0-day, evidencing that the acid extract of a culture of Lb. sakei 2a is a good technological alternative for the control of growth of L. monocytogenes in foods.

  3. Dynamic quantitative analysis of adherent cell cultures by means of lens-free video microscopy

    Science.gov (United States)

    Allier, C.; Vincent, R.; Navarro, F.; Menneteau, M.; Ghenim, L.; Gidrol, X.; Bordy, T.; Hervé, L.; Cioni, O.; Bardin, S.; Bornens, M.; Usson, Y.; Morales, S.

    2018-02-01

    We present our implementation of lens-free video microscopy setup for the monitoring of adherent cell cultures. We use a multi-wavelength LED illumination together with a dedicated holographic reconstruction algorithm that allows for an efficient removal of twin images from the reconstructed phase image for densities up to those of confluent cell cultures (>500 cells/mm2). We thereby demonstrate that lens-free video microscopy, with a large field of view ( 30 mm2) can enable us to capture the images of thousands of cells simultaneously and directly inside the incubator. It is then possible to trace and quantify single cells along several cell cycles. We thus prove that lens-free microscopy is a quantitative phase imaging technique enabling estimation of several metrics at the single cell level as a function of time, for example the area, dry mass, maximum thickness, major axis length and aspect ratio of each cell. Combined with cell tracking, it is then possible to extract important parameters such as the initial cell dry mass (just after cell division), the final cell dry mass (just before cell division), the average cell growth rate, and the cell cycle duration. As an example, we discuss the monitoring of a HeLa cell cultures which provided us with a data-set featuring more than 10 000 cell cycle tracks and more than 2x106 cell morphological measurements in a single time-lapse.

  4. Importance in dairy technology of bacteriocins produced by dairy starter cultures

    Directory of Open Access Journals (Sweden)

    Bedia Şimşek

    2002-03-01

    Full Text Available Bacteriocins produced by Lactic acid bacteria (LAB and propionic acid bacteria (PAB are heterogeneous group of peptide inhibitors which include lantibiotics (class I, e. g. nisin, small heat-stable peptides (class II, e. g. pediocin PA-1 and large heat-labile proteins (class III, e. g. helveticin J. Many bacteriocins belonging to the first two groups can be successfully used to inhibit undesirable microorganisms in foods, but only nisin is produced industrially and is used as a food preservative. LAB and PAB develops easily in milk and milk products. LAB and PAB growth in dairy products can cause microbial interference to spoilage and pathogenic bacteria through several metabolits, specially bacteriocins. The review deals with the description of milk-borne bacteriocins and their application in milk and milk products either to extend the shelf life or to inhibit milk pathogens.

  5. Essentialism, hybridism and cultural critique

    DEFF Research Database (Denmark)

    Frello, Birgitta

    2007-01-01

    to social and cultural critique. Through a critical discussion of the concept of hybridity, I argue that rather than expecting to find definite emancipating or suppressing capacities connected to constructions of the ‘hybrid' and the ‘pure', we should focus on how these two poles are invested with meaning...... and related to power. Hence, while insisting on Cultural Studies' commitment to social and cultural critique, I argue that this critique would benefit from an analytical sensitivity towards the uses and abuses of the discursive power to designate meaningful and legitimate subject positions, rather than...

  6. Organizational culture in the primary healthcare setting of Cyprus.

    Science.gov (United States)

    Zachariadou, Theodora; Zannetos, Savvas; Pavlakis, Andreas

    2013-03-24

    The concept of organizational culture is important in understanding the behaviour of individuals in organizations as they manage external demands and internal social changes. Cyprus healthcare system is under restructuring and soon a new healthcare scheme will be implemented starting at the Primary Healthcare (PHC) level. The aim of the study was to investigate the underlying culture encountered in the PHC setting of Cyprus and to identify possible differences in desired and prevailing cultures among healthcare professionals. The population of the study included all general practitioners (GPs) and nursing staff working at the 42 PHC centres throughout the island. The shortened version of the Organizational Culture Profile questionnaire comprising 28 statements on organizational values was used in the study. The instrument was already translated and validated in Greek and cross-cultural adaptation was performed. Participants were required to indicate the organization's characteristic cultural values orientation along a five-point Likert scale ranging from "Very Much = 1" to "Not at all= 5". Statistical analysis was performed using SPSS 16.0. Student t-test was used to compare means between two groups of variables whereas for more than two groups analysis of variance (ANOVA) was applied. From the total of 306 healthcare professionals, 223 participated in the study (72.9%). The majority of participants were women (75.3%) and mean age was 42.6 ± 10.7 years. Culture dimension "performance orientation" was the desired culture among healthcare professionals (mean: 1.39 ± 0.45). "Supportiveness" and "social responsibility" were the main cultures encountered in PHC (means: 2.37 ± 0.80, 2.38 ± 0.83). Statistical significant differences were identified between desired and prevailing cultures for all culture dimensions (p= 0.000). This was the first study performed in Cyprus assessing organizational culture in the PHC setting. In the forthcoming health system reform

  7. Workplace culture in psychiatric nursing described by nurses.

    Science.gov (United States)

    Kurjenluoma, K; Rantanen, A; McCormack, B; Slater, P; Hahtela, N; Suominen, T

    2017-12-01

    This study looks to describe the workplace culture from the viewpoints of stress, job satisfaction and practice environment. Data were collected from nurses (n = 109) using a web-based survey, The Person-Centred Nursing Index, from two purposefully selected hospital districts in Finland. Data were statistically analysed. Nurses described their workplace culture in slightly positive terms. Nurses only occasionally experienced stress (mean = 2.56, SD = 0.55) and were fairly satisfied with their job (mean = 4.75, SD = 0.66) and their practice environment (mean = 4.42, SD = 0.81). Demographic variables such as the nurses' age, length of time in nursing, time at their present hospital, working shifts and their use of patient restriction were more frequently associated with their perceived workplace culture. Older nurses and those with a longer work history in the nursing profession tended to be more satisfied with their workplace culture in psychiatric nursing. Young and/or newly graduated nurses felt more negatively on their workplace culture; this issue should be recognised and addressed with appropriate support and mentoring. Nurses who used restrictive measures were more often less satisfied with their workplace culture. Continuous efforts are needed to reduce the use of coercive measures, which challenge also the managers to support nursing practice to be more person-centred. © 2017 Nordic College of Caring Science.

  8. Promotion of healthy food by means of design

    Directory of Open Access Journals (Sweden)

    Kramskaia N. V.

    2018-01-01

    Full Text Available this article briefly considered the problem of healthy food, culture of food and influence of design as a culture on the positive perception of useful products. The comparative analysis of the American fast food and the Japanese sushi (the most common forms of fast food in our country are carried out for identification of design means at the expense of which the attractive image of food is formed and the visual harmony is reached. Several versions of the approved design decisions for achievement of desirable taste perception are also offered.

  9. Picroside I and Picroside II from Tissue Cultures of Picrorhiza kurroa

    Science.gov (United States)

    Ganeshkumar, Yamjala; Ramarao, Ajmera; Veeresham, Ciddi

    2017-01-01

    Background: Picrorhiza kurroa (PK) belongs to Scrophulariaceae family and is a representative endemic, medicinal herb, widely distributed throughout the higher altitudes of alpine Himalayas from west to east, between 3000 and 4500 m above mean sea level. Objective: The objective of the present study is to assess the production of picroside I and picroside II from tissue cultures of PK. Materials and Methods: Auxiliary shoot tips of PK were incubated in Murashige and Skoog medium supplemented with indole-3-butyric acid and kinetin phytohormones. The callus produced was collected at different time intervals and was processed for extraction of picroside I and picroside II followed by thin layer chromatography and high-performance liquid chromatography HPLC analysis. Results: The maximum growth index was found to be 5.109 ± 0.159 at 16-week-old callus culture. The estimation of picroside-I and picroside-II was carried out by (HPLC) analysis; quantity of secondary metabolite found to be 16.37 ± 0.0007 mg/g for PK-I and 6.34 ± 0.0012 mg/g for PK-II. Conclusion: This is the first attempt to produce the Picroside-I and II in large amount by the tissue culture technique. It can be observed that the method of callus culture can be used in production of secondary metabolites Picroside-I and II from PK SUMMARY Picrorhiza kurroa is a high value medicinal herb due to rich source of hepatoprotective metabolites, Picroside-I and Picroside-II. The medicinal importance of P. kurroa is due to its pharmacological properties like hepatoprotective, antioxidant (particularly in liver), antiallergic and antiasthamatic, anticancer activity particularly in liver and immunomodulatory. Shoot apices which were produced a good response was inoculated on selected medium i.e., on MS medium containing 2, 4 D (mg/l) + KN (1mg/l) for induction of callus. The initiation of callus was observed after 4weeks and it was light green and fragile Maximum growth was observed with 3% w/v of sucrose

  10. Examples of safety culture practices

    International Nuclear Information System (INIS)

    1997-01-01

    This report has been prepared to illustrate the concepts and principles of safety culture produced in 1991 by the International Safety Advisory Group as 75-INSAG-4. It provides a small selection of examples taken from a worldwide collection of safety performance evaluations (e.g. IAEA safety series, national regulatory inspections, utility audits and a plant assessments). These documented evaluations collectively provide a database of safety performance strengths and weakness, and related safety culture observations. The examples which have been selected for inclusion in this report are those which are considered worthy of special mention and which illustrate a specific attribute of safety culture given in 75-INSAG-4

  11. Emerging Perspectives on the Natural Microbiome of Fresh Produce Vegetables

    Directory of Open Access Journals (Sweden)

    Colin R. Jackson

    2015-04-01

    Full Text Available Plants harbor a diverse microbiome existing as bacterial populations on the leaf surface (the phyllosphere and within plant tissues (endophytes. The composition of this microbiome has been largely unexplored in fresh produce vegetables, where studies have tended to focus on pathogen detection and survival. However, the application of next-generation 16S rRNA gene sequencing approaches is beginning to reveal the diversity of this produce-associated bacterial community. In this article we review what is known about the composition of the microbiome of fresh produce vegetables, placing it in the context of general phyllosphere research. We also demonstrate how next-generation sequencing can be used to assess the bacterial assemblages present on fresh produce, using fresh herbs as an example. That data shows how the use of such culture-independent approaches can detect groups of taxa (anaerobes, psychrophiles that may be missed by traditional culture-based techniques. Other issues discussed include questions as to whether to determine the microbiome during plant growth or at point of purchase or consumption, and the potential role of the natural bacterial community in mitigating pathogen survival.

  12. Cultural Employment by Level of Education

    Directory of Open Access Journals (Sweden)

    Diana Sava

    2017-09-01

    Full Text Available The sector of the creative economy brings benefits to the economy, more exactly, through the cultural sector, due to the unlimited resources needed to develop – the human resources represented by their mind or talent. The industrial development and innovation lead towards many changes in the cultural industries mostly due to the digitization effect, an irreversible change in the creation of various cultural goods and services, resulting even new cultural domains and also new regulation in the cultural field. The goods and services produced by the new cultural sector “encompass artistic, aesthetic, symbolic and spiritual values (… their system of valorisation, which includes a characteristic irreproducible, is linked to its appreciation or pleasure” makes them different from other goods and services as Throsby mentioned (UNESCO, 2009:22. This paper aims to show some positive impacts of the creative economy, highlighting social and economic aspects, such as cultural diversity, tolerance, freedom of expression of the cultural identity and by the other hand, new jobs for artists, earnings’ increases, creative clusters, cultural employment etc. We will also show more clearly the activities and the occupations which concern the cultural employment and figures regarding cultural employment in Europe.

  13. The human and the inhuman: visual culture, political culture, and the images produced by George Rodger and Henri Cartier-Bresson in the Nazi concentration camps

    Directory of Open Access Journals (Sweden)

    Erika Cazzonatto Zerwes

    2016-08-01

    Full Text Available This article aims to grasp some aspects of the notion of humanism in photography and its closeness to the political culture and the visual culture in the period, through the specific experiences of George Rodger and Henri Cartier-Bresson, two photographers who were first-hand witnesses and provided accounts of horror in the Nazi concentration camps at the end of World War II. George Rodger photographed the Bergen-Belsen camp as soon as it was liberated by the British troops. Henri Cartier-Bresson was there with a film crew recording the deported masses newly freed from the Nazi concentration and extermination camps. These experiences came to have profound impact on the biography and work of both of them. In the two cases, there is a notion of humanism linked to World War II events, which is observed in photography and photographic representation, and it has a significant consequence for the contemporary visual culture.   Keywords: Visual Culture; Political Culture; War Photography; Photojournalism; Concentration Camps.   Original title: O humano e o desumano: cultura visual, cultura política e as imagens feitas por George Rodger e Henri Cartier-Bresson nos campos de concentração nazistas.

  14. The Linguistic and Cultural Interpretation of Dissonant Heritage: the ATRIUM Cultural Route

    Directory of Open Access Journals (Sweden)

    Sandra Nauert

    2017-06-01

    Full Text Available The European Cultural Route ATRIUM (Architecture of Totalitarian Regimes in Europe's Urban Memory deals with dissonant heritage, referring to a contrast of meaning and value systems between the past and the present. The route will be examined within the framework of a set of communication strategies for cultural routes. This short paper will look at some linguistic aspects related to the ATRIUM route and its ‘dissonant heritage’. By analysing some examples of existing promotional approaches related to this particular heritage, it will outline some considerations for a communicative strategy appropriate to the overall narrative structure of the ATRIUM cultural route, with special regard to a bottom-up constructivist approach.

  15. Soil and culture viewed through the prism of viticultural terroir

    Science.gov (United States)

    Vaudour, Emmanuelle

    2017-04-01

    Soil is at the heart of 'terroir', a French word with no full equivalent in other languages, meaning delimited areas with homogeneous environmental features that are likely to confer typical wine qualities identified through collective memory and conveyed from generation to generation within a territory marked by social context and crop technical choices (Vaudour, 2002, 2003). The tradition of terroir wines has strong cultural connections, referring to tradition of drinking well, of farming and of producing typical wines that are rooted in a region or made from specific places with organoleptic features easily distinguishable from other wines from other regions. As an entity distributed over space and time, terroir has cultural aspects that have heritage, landscape and reputation value-added components that come from historical, empirically derived technical adjustments, the transmission of taste typicity over generations and strong gastronomical traditions. As such, terroir has long been a source of inspiration for poetry, literature, and art in overall. Its tradition is also marked by the inheritance of long-standing practices of soil conservation and soil management. The tradition of terroir wines is a living tradition with positive values for the conservation of both cultural and soil patrimonies, that is likely to be shared by several winegrowing or wine-drinking countries.

  16. Vygotsky's Analysis of Children's Meaning Making Processes

    Science.gov (United States)

    Mahn, Holbrook

    2012-01-01

    Vygotsky's work is extensive and covers many aspects of the development of children's meaning-making processes in social and cultural contexts. However, his main focus is on the examination of the unification of speaking and thinking processes. His investigation centers on the analysis of the entity created by this unification--an internal…

  17. Interferon induction in bovine and feline monolayer cultures by four bluetongue virus serotypes.

    OpenAIRE

    Fulton, R W; Pearson, N J

    1982-01-01

    The interferon inducing ability of bluetongue viruses was studied in bovine and feline monolayer cultures inoculated with each of four bluetongue virus serotypes. Interferon was assayed by a plaque reduction method in monolayer cultures with vesicular stomatitis virus as challenge virus. Interferon was produced by bovine turbinate, Georgia bovine kidney, and Crandell feline kidney monolayer cultures in response to bluetongue virus serotypes 10, 11, 13 and 17. The antiviral substances produced...

  18. Culture of equine fibroblast-like synoviocytes on synthetic tissue scaffolds towards meniscal tissue engineering: a preliminary cell-seeding study

    Directory of Open Access Journals (Sweden)

    Jennifer J. Warnock

    2014-04-01

    Full Text Available Introduction. Tissue engineering is a new methodology for addressing meniscal injury or loss. Synovium may be an ideal source of cells for in vitro meniscal fibrocartilage formation, however, favorable in vitro culture conditions for synovium must be established in order to achieve this goal. The objective of this study was to determine cellularity, cell distribution, and extracellular matrix (ECM formation of equine fibroblast-like synoviocytes (FLS cultured on synthetic scaffolds, for potential application in synovium-based meniscal tissue engineering. Scaffolds included open-cell poly-L-lactic acid (OPLA sponges and polyglycolic acid (PGA scaffolds cultured in static and dynamic culture conditions, and PGA scaffolds coated in poly-L-lactic (PLLA in dynamic culture conditions.Materials and Methods. Equine FLS were seeded on OPLA and PGA scaffolds, and cultured in a static environment or in a rotating bioreactor for 12 days. Equine FLS were also seeded on PGA scaffolds coated in 2% or 4% PLLA and cultured in a rotating bioreactor for 14 and 21 days. Three scaffolds from each group were fixed, sectioned and stained with Masson’s Trichrome, Safranin-O, and Hematoxylin and Eosin, and cell numbers and distribution were analyzed using computer image analysis. Three PGA and OPLA scaffolds from each culture condition were also analyzed for extracellular matrix (ECM production via dimethylmethylene blue (sulfated glycosaminoglycan assay and hydroxyproline (collagen assay. PLLA coated PGA scaffolds were analyzed using double stranded DNA quantification as areflection of cellularity and confocal laser microscopy in a fluorescent cell viability assay.Results. The highest cellularity occurred in PGA constructs cultured in a rotating bioreactor, which also had a mean sulfated glycosaminoglycan content of 22.3 µg per scaffold. PGA constructs cultured in static conditions had the lowest cellularity. Cells had difficulty adhering to OPLA and the PLLA

  19. High Triterpenic Acids Production in Callus Cultures from Fruit Pulp of Two Apple Varieties.

    Science.gov (United States)

    Verardo, Giancarlo; Gorassini, Andrea; Ricci, Donata; Fraternale, Daniele

    2017-01-01

    Very rarely fruit pulp has been used in in vitro culture to produce secondary metabolites useful in promoting health. The aims of this work were the study of the best conditions to obtain the callus cultures from the pulp of two varieties of apples, Golden Delicious (GD) and "Mela Rosa Marchigiana" (MRM), and the quali-quantitative analysis of secondary metabolites produced by the two in vitro callus cultures. Callus was induced on both Murashige and Skoog and Gamborg B5 media containing various combinations of supplements. To achieve the maximum recovery of secondary metabolites produced, preliminary extraction tests were carried out on GD apple culture using two different organic solvents (MeOH and EtOAc). The quali-quantitative analysis of the methanolic extract of both cultures was carried out by ESI-MS n and GC-MS techniques. The GC-MS analysis revealed the presence of triterpenic acids, in particular, oleanolic, ursolic, maslinic, pomolic, tormentic, corosolic and annurcoic acid along with a phytosterol, β-sitosterol. In addition, GD callus culture produced phloridzin, absent in the MRM culture. In this last culture, however, the total amount of secondary metabolites was markedly higher. The in vivo production of these bioactive compounds were also quantified in the GD and MRM apple pulps. Apple pulps produced higher amounts of triterpenic acids in vitro than in vivo. The present work can be considered a method to amplify the production of important secondary metabolites which exert beneficial effects on human health. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  20. Metal-chelating compounds produced by ectomycorrhizal fungi collected from pine plantations.

    Science.gov (United States)

    Machuca, A; Pereira, G; Aguiar, A; Milagres, A M F

    2007-01-01

    To investigate the in vitro production of metal-chelating compounds by ectomycorrhizal fungi collected from pine plantations in southern Chile. Scleroderma verrucosum, Suillus luteus and two isolates of Rhizopogon luteolus were grown in solid and liquid modified Melin-Norkans (MMN) media with and without iron addition and the production of iron-chelating compounds was determined by Chrome Azurol S (CAS) assay. The presence of hydroxamate and catecholate-type compounds and organic acids was also investigated in liquid medium. All isolates produced iron-chelating compounds as detected by CAS assay, and catecholates, hydroxamates as well as oxalic, citric and succinic acids were also detected in all fungal cultures. Scleroderma verrucosum produced the greatest amounts of catecholates and hydroxamates whereas the highest amounts of organic acids were detected in S. luteus. Nevertheless, the highest catecholate, hydroxamate and organic acid concentrations did not correlate with the highest CAS reaction which was observed in R. luteolus (Yum isolate). Ectomycorrhizal fungi produced a variety of metal-chelating compounds when grown in liquid MMN medium. However, the addition of iron to all fungi cultures reduced the CAS reaction, hydroxamate and organic acid concentrations. Catecholate production was affected differently by iron, depending on the fungal isolate. The ectomycorrhizal fungi described in this study have never been reported to produce metal-chelating compound production. Moreover, apart from some wood-rotting fungi, this is the first evidence of the presence of catecholates in R. luteolus, S. luteus and S. verrucosum cultures.

  1. ROMANIAN BALKANISM OR ABOUT CULTURAL RIFTS

    Directory of Open Access Journals (Sweden)

    Petre Gheorghe BÂRLEA

    2013-05-01

    Full Text Available The term “Balkanism” has two meanings, referring to both something objective and strictly geographic, as well as something subjective – a meaning that describes a certain mentality, a certain manner of thinking and behavior embraced in the Balkans. This article sets out to analyse the elements that are considered to define the cultural identity of the communities from the area of the Balkans. Their historical events, their linguistic differences and peculiarities, their art (music, cinematography, etc., their culinary p references and other elements are approached and described from the perspective of the “cultural crevice” theory.

  2. Community health workers as cultural producers in addressing gender-based violence in rural South Africa.

    Science.gov (United States)

    de Lange, Naydene; Mitchell, Claudia

    2016-01-01

    South Africa has been experiencing an epidemic of gender-based violence (GBV) for a long time and in some rural communities health workers, who are trained to care for those infected with HIV, are positioned at the forefront of addressing this problem, often without the necessary support. In this article, we pose the question: How might cultural production through media making with community health workers (CHWs) contribute to taking action to address GBV and contribute to social change in a rural community? This qualitative participatory arts-based study with five female CHWs working from a clinic in a rural district of South Africa is positioned as critical research, using photographs in the production of media posters. We offer a close reading of the data and its production and discuss three data moments: CHWs drawing on insider cultural knowledge; CHWs constructing messages; and CHWs taking action. In our discussion, we take up the issue of cultural production and then offer concluding thoughts on 'beyond engagement' when the researchers leave the community.

  3. Strategies of development of European power producers

    International Nuclear Information System (INIS)

    Heddebaut, D.

    2007-12-01

    This economical study answers the following questions: what is the dynamics of European power markets? What is the relative competitiveness of the different power production means? What are the determining factors of electricity wholesale prices? Who are the operators the most threatened by the rise of wholesale prices? To what extend power producers are impacted by the CO 2 constrain? Who are the most efficient producers? The analysis is based on 18 of the most representative groups of the European electric utilities. The first volume of the study presents the strategic perspectives of European power producers: dynamics and basic characteristics of European power markets, strategic options of European operators, typology and financial results of European power producers, scenarios of market reorganization according to Eurostaf (possible mega-mergers in the future, competition). Volume 2 makes a detailed analysis of the positioning and perspectives of the 18 selected groups: key figures, sourcing strategies, production means management, Eurostaf's diagnosis. (J.S.)

  4. Safety culture in nuclear installations. Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Carnino, A [ed.; International Atomic Energy Agency, Vienna (Austria); Weimann, G [ed.; Oesterreichisches Forschungszentrum Seibersdorf GmbH (Austria)

    1995-04-01

    These proceedings of the International Topical Meeting on Safety Culture in Nuclear Installations held in Vienna, Austria from 24 to 28 April 1995 provide a wide forum of information exchange and discussions on the topic safety culture in nuclear power plants. Safety culture deals with human factors since it deals with attitudes, organization and management. It then means that it has a natural component in it which is linked to the national culture and education. There are about 95 contributions, some of them presented by title and abstract only. All of them are in the subject scope of INIS. (Botek).

  5. Safety culture in nuclear installations. Proceedings

    International Nuclear Information System (INIS)

    Carnino, A.; Weimann, G.

    1995-04-01

    These proceedings of the International Topical Meeting on Safety Culture in Nuclear Installations held in Vienna, Austria from 24 to 28 April 1995 provide a wide forum of information exchange and discussions on the topic safety culture in nuclear power plants. Safety culture deals with human factors since it deals with attitudes, organization and management. It then means that it has a natural component in it which is linked to the national culture and education. There are about 95 contributions, some of them presented by title and abstract only. All of them are in the subject scope of INIS. (Botek)

  6. Organizational climate and culture.

    Science.gov (United States)

    Schneider, Benjamin; Ehrhart, Mark G; Macey, William H

    2013-01-01

    Organizational climate and organizational culture theory and research are reviewed. The article is first framed with definitions of the constructs, and preliminary thoughts on their interrelationships are noted. Organizational climate is briefly defined as the meanings people attach to interrelated bundles of experiences they have at work. Organizational culture is briefly defined as the basic assumptions about the world and the values that guide life in organizations. A brief history of climate research is presented, followed by the major accomplishments in research on the topic with regard to levels issues, the foci of climate research, and studies of climate strength. A brief overview of the more recent study of organizational culture is then introduced, followed by samples of important thinking and research on the roles of leadership and national culture in understanding organizational culture and performance and culture as a moderator variable in research in organizational behavior. The final section of the article proposes an integration of climate and culture thinking and research and concludes with practical implications for the management of effective contemporary organizations. Throughout, recommendations are made for additional thinking and research.

  7. Aroma characteristics of Moutai-flavour liquor produced with Bacillus licheniformis by solid-state fermentation.

    Science.gov (United States)

    Zhang, R; Wu, Q; Xu, Y

    2013-07-01

    The potential of Bacillus licheniformis as a starter culture for aroma concentration improvement in the fermentation of Chinese Moutai-flavour liquor was elucidated. The volatile compounds produced by B. licheniformis were identified by GC-MS, in which C4 compounds, pyrazines, volatile acids, aromatic and phenolic compounds were the main ingredients. The strains B. licheniformis (MT-6 and MT-15) produced more volatile compound concentrations, mainly C4 compounds, than the type strain of B. licheniformis (ATCC 14580) at the fermentation temperature of 55°C. Meanwhile, more volatile compound concentrations were produced by B. licheniformis in solid-state fermentation than in submerged state fermentation. Thus, the strains MT-6 and MT-15 were used as the Bacillus starter culture for investigating Moutai-flavour liquor production. The distilled liquor inoculated with Bacillus starter culture was significantly different from the liquor without inoculum. This was particularly evident in the fore-run part of the distilled sample which was inoculated with Bacillus starter culture, where volatile compounds greatly increased compared to the control. Furthermore, the distilled liquor with Bacillus starter culture showed improved results in sensory appraisals. These results indicated that B. licheniformis was one of the main species influencing the aroma characteristics of Moutai-flavour liquor. This is the first report of an investigation into the effect of Bacillus starter cultures on the flavour features of Moutai-flavour liquor, which verified that Bacillus licheniformis can enhance aroma concentration in Moutai-flavour liquor. Bacillus starter culture brought C4 compounds, pyrazines, volatile acids, aromatic and phenolic compounds to the liquor, which gave a better result in sensory appraisals. © 2013 The Society for Applied Microbiology.

  8. Bastard Culture! User participation and the extension of the cultural industries

    NARCIS (Netherlands)

    Schäfer, M.T.

    2008-01-01

    Over the past decade a culture of user participation has developed on a global scale contributing to the development of software as well as changing, commenting, creating and distributing media content. With the personal computer, the internet and software, users have powerful production means at

  9. Between history and cultural psychology

    DEFF Research Database (Denmark)

    Brescó, Ignacio

    2016-01-01

    Innis’ and Brinkmann’s papers (this issue) tackle two key aspects in cultural psychology: the mediating role played by the different systems of meanings throughout history in making sense of the world, and the normative role of those systems, including psychology itself. This paper offers...... a reflection on these two issues. It begins by highlighting the contribution of psychology and history, as emerging disciplines in the 19th Century, to the creation of a normative framework for the subject of modernity according to the needs of modern nation states. It also alludes to both disciplines’ common...... accounts. Drawing on this assumption, it is discussed how past events are constructed, thus bringing mediation and meaning-making to the fore. Special attention is paid to narratives as symbolic meaning-making tools. We will conclude by discussing usage of the past and the role that cultural psychology can...

  10. Beta-Lactamase Producing Escherichia coli Isolates in Imported and Locally Produced Chicken Meat from Ghana

    DEFF Research Database (Denmark)

    Rasmussen, Mette Marie; Opintan, Japheth A; Frimodt-Møller, Niels

    2015-01-01

    whether imported chicken meat and meat from locally reared chicken are potential sources for human exposure to multi resistant Escherichia coli isolates. 188 samples from imported and locally produced chicken meat were sampled and analyzed. 153 bacteria isolates were successfully cultured and identified...... phenotypically confirmed on all isolates showing resistance to cefpodoxime. Beta-lactamase producing (BLP) E. coli meat isolates were further genotyped. Antimicrobial resistance to four antibiotic markers with highest resistance was detected more frequently in isolates from local chickens compared to imported......The use of antibiotics in food animals is of public health concern, because resistant zoonotic pathogens can be transmitted to humans. Furthermore, global trade with food may rapidly spread multi-resistant pathogens between countries and even continents. The purpose of the study was to investigate...

  11. Theory and Method in Cross-Cultural Psychology

    Science.gov (United States)

    Malpass, Roy S.

    1977-01-01

    Cross cultural psychology is considered as a methodological strategy, as a means of evaluating hypotheses of unicultural origins with evidence of more panhuman relevance, and as a means of developing new theoretical psychological phenomena. (Author)

  12. Culture phenomenon analysis on the forest tour activity of China

    Institute of Scientific and Technical Information of China (English)

    Jiang Minjin

    2006-01-01

    This paper analyzes culture and forest culture, the intension of culture and forest culture, combines the understanding of the main cultural factor with the forest tour activity of China, analyzes the compatible phenomenon of Chinese forest culture and traditional culture, and explores culture of forest tourist site containing the meaning in forest tour. The author thinks the tour of forest culture which will be the important component of forest tour in forest culture,This paper puts forward simple questions existing in exploitation and advantage of forest tour culture, and proposes some countermeasures.

  13. Production and novel quantification of haemolysins produced by coagulase-negative staphylococci isolated from subclinical mastitis in sheep.

    Science.gov (United States)

    Kanellos, T S; Burriel, A R

    2002-07-01

    Coagulase-negative staphylococci producing cell-damaging toxins were isolated from the milk of sheep with subclinical mastitis. The haemolytic activity of Coagulase-negative staphylococcal strains was assessed on solid and liquid culture media. More than 61% and 76% of the tested strains on solid media produced evidence of alpha- and delta- haemolysins and more than 78% produced synergistic haemolysis. However almost all isolates producing haemolysin in liquid culture media produced only very few units of haemolysin compared to the positive control of five Coagulase-positive strains of staphylococci. It was concluded that solid media are better for classifying Coagulase-negative staphylococci as producers or not of haemolysins, and liquid media for measuring the size of this activity within the first few hours of intramammary infection.

  14. Multi-lipofection efficiently transfected genes into astrocytes in primary culture.

    Science.gov (United States)

    Wu, B Y; Liu, R Y; So, K L; Yu, A C

    2000-10-30

    This study demonstrated that liposome-mediated transfection - lipofection - is suitable for delivering genes into astrocytes. By repeatedly lipofecting the same astrocyte cultures, a process we call multi-lipofection, the transfection efficiency of the beta-galactosidase (beta-gal) gene was improved from 2.6+/-0.6 to 17. 4+/-1.1%. This is the highest efficiency ever reported in gene-transfer with Lipofectin(R) in a primary culture of mouse cerebral cortical astrocytes. Furthermore, multi-lipofection did not cause observable disturbance to astrocytes as indicated by insignificant changes in the glial fibrillary acidic protein content in the cultures. In order to demonstrate that the transfected gene achieved a physiologically relevant expression level, a plasmid containing the pEF-hsp70 protein gene was lipofected into astrocytes. This produced colonies of astrocytes showing an increased resistance to heat-induced cell death. A similar experiment was performed with the glial-derived neurotrophic factor (GDNF) gene. Control astrocytes had no detectable GDNF. In the transfected astrocytes, the GDNF protein could be identified intracellularly by immunocytochemistry. Western blot analysis revealed, as compared to astrocytes with one lipofection, a 2.9-fold increase of GDNF with four lipofections. GDNF remained detectable in astrocytes 2 weeks after four lipofections. Thus, multi-lipofection provides a mild and efficient means of delivering foreign genes into astrocytes in a primary culture, making astrocytes good candidate vehicle cells for gene/cell therapy in the CNS.

  15. The Politics of Cultural Difference in Second Language Education

    Science.gov (United States)

    Kubota, Ryuko

    2004-01-01

    Cultural difference is an important topic of discussion in second language education. Yet cultural difference is often conceptualized as fixed, objective, and apolitical based on an essentialist and normative understanding of culture. This article challenges such conceptualizations by examining and politicizing multiple and conflicting meanings of…

  16. Cloning higher plants from aseptically cultured tissues and cells

    Science.gov (United States)

    Krikorian, A. D.

    1982-01-01

    A review of aseptic culture methods for higher plants is presented, which focuses on the existing problems that limit or prevent the full realization of cloning plants from free cells. It is shown that substantial progress in clonal multiplication has been made with explanted stem tips or lateral buds which can be stimulated to produce numerous precocious axillary branches. These branches can then be separated or subdivided and induced to root in order to yield populations of genetically and phenotypically uniorm plantlets. Similarly, undifferentiated calluses can sometimes be induced to form shoots and/or roots adventitiously. Although the cell culture techniques required to produce somatic embryos are presently rudimentary, steady advances are being made in learning how to stimulate formation of somatic or adventive embryos from totipotent cells grown in suspension cultures. It is concluded that many problems exist in the producing and growing of totipotent or morphogenetically competent cell suspensions, but the potential benefits are great.

  17. Yield improvement strategies for the production of secondary metabolites in plant tissue culture: silymarin from Silybum marianum tissue culture.

    Science.gov (United States)

    AbouZid, S

    2014-01-01

    Plant cell culture can be a potential source for the production of important secondary metabolites. This technology bears many advantages over conventional agricultural methods. The main problem to arrive at a cost-effective process is the low productivity. This is mainly due to lack of differentiation in the cultured cells. Many approaches have been used to maximise the yield of secondary metabolites produced by cultured plant cells. Among these approaches: choosing a plant with a high biosynthetic capacity, obtaining efficient cell line for growth and production of metabolite of interest, manipulating culture conditions, elicitation, metabolic engineering and organ culture. This article gives an overview of the various approaches used to maximise the production of pharmaceutically important secondary metabolites in plant cell cultures. Examples of using these different approaches are shown for the production of silymarin from Silybum marianum tissue culture.

  18. Aflatoxin B1 producing potential of Aspergillus flavus strains isolated ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-07-20

    Jul 20, 2009 ... consumption of aflatoxin contaminated food and feed. (Reddy and ..... intervals by artificial inoculation of A. parasiticus on rice grains. ... additive for culture media for rapid identification of aflatoxin producing. Aspergillus strains ...

  19. Culture in constructive remembering

    DEFF Research Database (Denmark)

    Wagoner, Brady

    2012-01-01

    an alternative temporal metaphor of remembering as ‘construction’. If we push his metaphor further—with the help of cultural psychology—we can say memory construction is done by agents using cultural ‘tools’ such as language and narrative. In this chapter, Bartlett’s theory is contextualized, elucidated......, critiqued and developed with the help of a number of other thinkers. The ultimate aim of the chapter is to go beyond Bartlett and arrive at a thoroughgoing culturally inclusive psychological theory of remembering. Though Bartlett clearly situated remembering within a social process, he did not provide......The present chapter explores novel ways of thinking about what it means to remember and how precisely culture is involved in this process. Since Plato, the dominant metaphor for conceptualizing memory has been that of a spatial ‘storage’. In contrast to this, Frederic Bartlett advanced...

  20. What Physicist Mean By The Equals Sign In Undergraduate Education

    Science.gov (United States)

    Zohrabi Alaee, Dina; Kornick, Kellianne; Sayre, Eleanor C.; Franklin, Scott V.

    2017-01-01

    Mathematical concepts and tools have an important role in physics. Faculties want students to think critically about mathematics and the underlying fundamental concepts, rather than simply memorizing a series of equations and answers. The equals sign - ubiquitous in problem solving - carries different conceptual meaning depending on how it is used; this meaning is deeply tied to cultural practices in problem solving in physics. We use symbolic forms to investigate the conceptual and cultural meanings of the equals sign across physics contexts. We built and validated a rubric to classify the ways that physics students use the equals sign in their written work. Our categories are causality, assignments, definitional, balancing, and just math. We analyze students' use of the equals sign in their written homework and exam solutions in an upper-division electrostatics course. We correlate the kinds of equal signs within problem solutions with the difficulty of the problem. We compare they ways students use the equals sign to their course lectures and textbook.