WorldWideScience

Sample records for culturally produced meanings

  1. Music, Meaning and Culture

    Directory of Open Access Journals (Sweden)

    Richard Widdess

    2012-09-01

    Full Text Available This paper situates musical meaning in culture, addressing music as social symbol and as ongoing process of meaning creation. Three examples of non- Western musical practice are used to illustrate the embedding of musical meaning in cultural context. The performance of an Australian Aboriginal song is shown to exemplify the interdependence of song style and social structure as a matrix for the emergence of cultural meanings; an example of North Indian performance is adduced to demonstrate the multi-layered nature of meaning as embodied in musical performance; and an example of collective festival performance from Nepal illustrates ways in which the structure of musical performance can mirror local cultural forms. Each of the three examples lends weight to the idea that music's meanings are often non-linguistic and reflect foundational schemas that are specific to the cultures from the musics are drawn.

  2. Culture as meaning-making

    OpenAIRE

    Lenkauskienė, Rūta; Liubinienė, Vilmantė

    2002-01-01

    The present paper analyses the role of social and cultural background knowledge in the cognition of meaning. Language and culture integrated studies have long been in the focus of attention. In order to study the language of a target culture, one should understand how human beings construct meanings, understand processes of meaning-making, account for different meanings, and examine their effects in social life. The language cannot be interpreted in the right way without taking the target cul...

  3. When means become ends: technology producing values

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    Bjørn Hofmann

    2006-12-01

    Full Text Available Technology has become the symbol of our culture.  The claim that we are subject to a technological imperative is therefore a fundamental cultural critique: we do not control technology, rather technology controls us. An alternative way to formulate this is to claim that technology cannot be “made down” when it is made up; we just have to make the best of it. Accordingly, it has been argued that technology has evolved from being merely a means to becoming an end in itself. This article investigates this claim by analyzing the relationship between technology and values. The examples stem from the technologies of medicine and weapons because they clarify this relationship. It is argued that technology relates to values in two ways. Technology both raises general questions about values and it is value-laden due to its very function. However, although technology is value-laden, it does not necessarily give an imperative mandate. One reason for this lies in our responsibility. We are inevitably responsible for all aspects of technology, i.e. development, construction, production, commercialization, implementation, and use. Referring to a technological imperative to explain and defend our decisions with respect to technology constitutes an unjustified renunciation of our own responsibility. Hence, the article tries to underscore our responsibility by developing a technological axiology.

  4. Musics, Cultures and Meanings: Music as Communication

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    Ian Cross

    2012-09-01

    Full Text Available This commentary explores interpretations of concepts that lie at the focus of Richard Widdess's paper—"music", and "culture"—with the aim of specifying frameworks within which issues of musical meaning can fruitfully be addressed.

  5. Meanings of health: interrogating structure and culture.

    Science.gov (United States)

    Dutta, Mohan Jyoti; Basu, Ambar

    2008-11-01

    Based on the argument that context ought to be centralized in discourses of health communication, this article applies the culture-centered approach to engage in dialogue about issues of health with 18 men in rural West Bengal. The culture-centered approach is based on dialogue between the researcher and the community members, with the goals of listening to the voices of cultural members in suggesting culture-based health solutions. In this project, our discursive engagement with the participants suggests that health is primarily constructed as an absence, framed in the realm of minimal access to healthcare resources. In a situation where the resources are limited, the participants discussed the importance of trust in their relationship with the local provider. Health was also seen as a collective resource that was both an asset of the collective and a responsibility of the collective. Finally, the participants also pointed out the ways in which corruption in the structure introduced a paradox in policy discourse and the material conditions of the participants.

  6. Cultural Meanings Construction: An Analysis of the Organic Grape Juice Market

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    Marlon Dalmoro

    2015-04-01

    Full Text Available Regarding the meanings networks that involve markets, this research aims to analyze cultural meanings production in the organic grape juice market, involving consumers and producers agents. It was adopted a qualitative approach with an interpretative character by interviews with 25 consumers and producers. Cultural meanings construction description and analysis involved the socio-cultural context, interaction between agents (producers and consumers and meanings assigned by each agent. Organics are meant as food for both agents. However, others meanings associated to organics operate in dichotomic levels. It results in a non-homogeneous network between producers and consumers, reflecting the different cultural universe of each agent. These results mainly contribute in understanding the patterns of production and consumption around the food market. The lack of consolidated networks of meanings limit the construction and establishment of organic products market structures.

  7. Flows of Meaning, Cultures of Movements

    DEFF Research Database (Denmark)

    Jensen, Ole B.

    2009-01-01

    Contemporary cities and places are defined by mobility and flows as much as by their sedentary and fixed properties. In the words of Shane the city may be seen as configured by ‘enclaves' (fixed and bounded sites) and ‘armatures' (infrastructure channels and transit spaces). By taking point...... of departure in a critique of such a sedentary / nomad dichotomy the paper aims to move beyond these arguing for a third position of ‘critical mobility thinking'. The theoretical underpinning of this position reaches across cultural theory, human geography and into sociology. It includes a notion...

  8. Narratives of continued formation: meanings produced by Physical Education Teachers

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    André da Silva Mello

    2015-06-01

    Full Text Available It discusses the meanings given by 14 physical education teachers about their experiences with the continued formation process. It is a (auto biographical narrative research, which used focal groups and interviews as tools for compiling the data. The colaborators are six male teachers and eight female teachers of municipal networks from Serra, Vitoria and Viana municipalities in the metropolitan region of Vitória, ES. The analyzes indicate that the assigned meanings are related to the collective need to reorganize institutional provided formations in dialogue with the political interests of teachers and the specificity of Physical Education as a curriculum component. In general, teachers consider the formation focused on practice and in the teaching profession as those that best reflect their expectations. The practice appears as a know-how to be appropriate from continued formetion to be experienced in school; a knowledge that, when apprehended, is reframed to produce other experiences.

  9. African-American Vernacular Dance: Core Culture and Meaning Operatives.

    Science.gov (United States)

    Hazzard-Gordon, Katrina

    1985-01-01

    Discusses the role of social dancing in the lives and culture of working class Black Americans. Focuses specifically on four aspects of its meaning: identity (self-esteem), cultural integrity, ingroup-outgroup, and political resistance. Bases argument on sociological, biographical, and fictional works by and about Black culture. (KH)

  10. Independent Learning Crossing Cultures: Learning Cultures and Shifting Meanings

    Science.gov (United States)

    Spiro, Jane; Henderson, Juliet; Clifford, Valerie

    2012-01-01

    This paper contrasts the notion of "independent learning" as perceived by two informant groups at a UK institution of higher education: (1) teachers, educators and providers of education and (2) their students or "consumers" of education. Both informant groups are staff and students studying in a culture different to that of…

  11. Greater learnability is not sufficient to produce cultural universals.

    Science.gov (United States)

    Rafferty, Anna N; Griffiths, Thomas L; Ettlinger, Marc

    2013-10-01

    Looking across human societies reveals regularities in the languages that people speak and the concepts that they use. One explanation that has been proposed for these "cultural universals" is differences in the ease with which people learn particular languages and concepts. A difference in learnability means that languages and concepts possessing a particular property are more likely to be accurately transmitted from one generation of learners to the next. Intuitively, this difference could allow languages and concepts that are more learnable to become more prevalent after multiple generations of cultural transmission. If this is the case, the prevalence of languages and concepts with particular properties can be explained simply by demonstrating empirically that they are more learnable. We evaluate this argument using mathematical analysis and behavioral experiments. Specifically, we provide two counter-examples that show how greater learnability need not result in a property becoming prevalent. First, more learnable languages and concepts can nonetheless be less likely to be produced spontaneously as a result of transmission failures. We simulated cultural transmission in the laboratory to show that this can occur for memory of distinctive items: these items are more likely to be remembered, but not generated spontaneously once they have been forgotten. Second, when there are many languages or concepts that lack the more learnable property, sheer numbers can swamp the benefit produced by greater learnability. We demonstrate this using a second series of experiments involving artificial language learning. Both of these counter-examples show that simply finding a learnability bias experimentally is not sufficient to explain why a particular property is prevalent in the languages or concepts used in human societies: explanations for cultural universals based on cultural transmission need to consider the full set of hypotheses a learner could entertain and all of

  12. Consumer Culture Theory: Ideology, Mythology and Meaning in Technology Consumption

    DEFF Research Database (Denmark)

    Bajde, Domen

    2014-01-01

    innovations, thus shaping the value of technologies as cultural resources sustaining consumer identities. In its urge to shed light on these aspects, CCT tends to reinforce the gaps and asymmetries between the “socio-cultural” and the “techno-material”, leaving plenty of room for further study. The authors......Consumer culture theory helps us take note of the cultural forces and dynamics in which technology consumption is entangled. It enables people to articulate the cultural processes (ideological, mythic, ritualistic, etc.) through which cultural meanings become granted to or denied to technological...

  13. THE MEANING OF HOME IN YORUBA CULTURE OKEYINKA, Y ...

    African Journals Online (AJOL)

    Osondu

    2012-10-03

    Oct 3, 2012 ... Key words: Meaning, House, Home, Yoruba culture, Cradle, Premium. Introduction ..... Amole, B., David, K. and Graham, T. (1993). The. Family House in west ... review of the Literature, The Sociological review,. Pp. 62 – 89.

  14. Cinema as Cultural Pedagogy: Meanings by Elderly Women

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    Wânia Ribeiro Fernandes

    2010-01-01

    Full Text Available Using the theoretical reference of the cultural studies- mainly some foucauldian and feminist studies notions- this paper focuses upon the influence of films on the construction of gender identities. After a brief discussion on aspects of culture, power and identities formation, the meanings established by a group of elderly women on films they have attended during their youth are analyzed. We bring into evidence the effects that thediscourses present in that media had upon those women’s identitary construction and production.

  15. What Psychoanalysis, Culture And Society Mean To Me

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    Layton, Lynne

    2007-01-01

    The paper reviews some ways that the social and psychic have been understood in psychoanalysis and argues that a model for understanding the relation between the psychic and the social must account both for the ways that we internalize oppressive norms as well as the ways we resist them. The author proposes that we build our identities in relation to other identities circulating in our culture and that cultural hierarchies of sexism, racism, classism push us to split off part of what it means to be human, thereby creating painful individual and relational repetition compulsions. These “normative unconscious processes” replicate the unjust social norms that cause psychic pain in the first place. The paper concludes with thoughts about contemporary US culture, in which the government has abdicated responsibility toward its most vulnerable citizens and has thus rendered vulnerability and dependence shameful states. PMID:22058628

  16. Spreadable media: Creating value and meaning in a networked culture

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    Moderated by Louisa Stein

    2014-09-01

    Full Text Available Online Roundtable on Spreadable Media, by Henry Jenkins, Sam Ford, and Joshua Green, with participants Paul Booth, Kristina Busse, Melissa Click, Sam Ford, Henry Jenkins, Xiaochang Li, and Sharon Ross. Section 1 first published as the article "Spreadable Media: Creating Value and Meaning in a Networked Culture", by Louisa Stein, from Cinema Journal Volume 53 Issue 3, pp152-177. Copyright 2014 by The University of Texas Press. All rights reserved.

  17. School Meaning Systems: The Symbiotic Nature of Culture and "Language-In-Use"

    Science.gov (United States)

    Abawi, Lindy

    2013-01-01

    Recent research has produced evidence to suggest a strong reciprocal link between school context-specific language constructions that reflect a school's vision and schoolwide pedagogy, and the way that meaning making occurs, and a school's culture is characterized. This research was conducted within three diverse settings: one school in the Sydney…

  18. Culture care meanings, beliefs, and practices in Rural Dominican Republic.

    Science.gov (United States)

    Schumacher, Gretchen

    2010-04-01

    This ethnonursing study explored the meanings, beliefs, and practices of care for rural Dominicans in the Dominican Republic. Leininger's culture care diversity and universality theory, ethnonursing, and four-phase analysis method guided the study. Interviews were conducted with 19 general and 10 key informants. Analysis of interviews revealed three main themes: (a) family presence is essential for meaningful care experiences and care practices, (b) respect and attention are central to the meaning of care and care practices, and (c) rural Dominicans value and use both generic (folk) and professional care practices. Implications and recommendations for nursing practice, education, and research are described.

  19. Cultural Meanings and Consumers’ Discourses about Their Brand Abandonment

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    Filipe Diniz

    2018-03-01

    Full Text Available Although we know a lot about how brand meanings are created and perpetuated in relation to and through cultural discourses, remarkably little work has been done in the marketing field to develop a better comprehension as to how brand meanings are enacted through distancing behaviors, such as brand abandonment. In the marketing literature, abandonment has usually been associated with relationship crises, most commonly as the result of consumer dissatisfaction. This study investigates consumers who abandoned previously consumed brands in two distinct product categories, soft drinks and automobiles. Through investigation of two emblematic brands – CocaCola and Fiat - the analysis illustrates cultural discourses that consumers use to give meanings and socially negotiate their brand abandonment. Considering the repertoire of meanings attached to both the brands and consumers’ commitment to their distancing behavior, the analysis presents three types of brand abandonment: (a contingent, (b balanced and (c aversive. This paper also presents brand abandonment as an enabler of social distinctions, highlighting two discrete discourses promulgated and perpetuated by consumers: (a Life evolution, and (b Rationality, self-control and sovereignty.

  20. THE MEANING IN THE CULTURE: BASIC CONCEPT TO ORGANIZATIONAL LEARNING

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    ÁLVARO ENRÍQUEZ MARTÍNEZ

    2007-01-01

    Full Text Available Based on three key psychological concepts: learning, culture and meaning, the present essay proposes a conceptualbase frame, oriented toward the understanding of development in organizations into current milieu ofcompetitiveness and temporality, of the relationships among people working into them. The organizations whichare typically embedded in a context of values, needs and symbols that made up their cultures, must evolve in orderto face the demands for new developments and change, to which they are forced to. The concept of “meaning of theculture”, is presented as the base over which is build and rooted the organizational learning - in a technical and socialsense. The different types of learning are the ways in which the organizations satisfy the requirements coming fromtheir environments, in front of which must generate knowledge and consequently, new products and services,based on the people that form such organizations. The people that belong and constitutes the organization, incircumstances of temporal cohesion and within temporary working networks, must achieve results and to beadjusted to these new working and organizational facts, developing self-management and autonomy, in order tosignify and get adapted into the cultural tissue.

  1. Cultural meanings of musculoskeletal diseases in Chile's Mapuche Population.

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    Alarcon, Ana M; Vidal, Aldo; Castro, Marcela

    2013-10-01

    Eight out of 10 Mapuche indigenous women have a musculoskeletal disorder (MSD) and do not seek early medical aid. To study both the cultural meanings and implications of MSD from the Mapuche worldview. Ethnographic study carried out from 2008 to 2011 on indigenous reserves in southern Chile. Sixty-four Mapuches participated in comprehensive interviews, which were transcribed and analyzed by the research team. Five cultural domains: (a) foro kutran/bone disease is the general denomination of MSD; (b) Re-Rume Kutran/progressive and incurable course, refers to the course of the disease; (c) Kalül fücha mawiza/body is an old tree, describes internal manifestations such as worn bones, dry body, weak blood, and spiritual weakness; (d) witrür tripai foro/deformation is the external manifestation of MSD; and (e) Reñma ka lof kutran/family and community suffering refers to the impact of MSD. The explanation of MSD is consistent with the integrated body-nature-spirit worldview of the Mapuche. To provide cultural nursing health care so that patients receive prompt diagnosis and care.

  2. Culture cultivating culture: the four products of the meaning-made world.

    Science.gov (United States)

    Carriere, Kevin R

    2014-09-01

    Culture, in a semiotic cultural psychology, is defined from the viewpoint of cultivation--the meaning making processes that give meaning to the world (Valsiner 2000, 2007a). However, the individual is not simply a process-machine in an empty world--there are both the external outcomes of meaning making (individual and group based) as well as the collective influence on the cultivation process. I argue to examine the cultivation process more completely, one must look at these external influences that catalyze future cultivation processes. By examining the power of the external (environmental Umwelten) and group-internal (myths, morals), a much greater understanding of the behavior of individuals can be accomplished beyond examining the individual's process of meaning making. Further work into examining the objects that affectively activate the individual as well as group action and meaning making is called for and examples of such studies are given.

  3. EDUCATIONAL QUEST AS MEANS OF FORMING OF INFORMATION CULTURE

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    В И Глизбург

    2016-12-01

    Full Text Available Creative use of the potential of modern information and communication technologies for solving pedagogical, educational and training tasks is now an urgent problem of education. It is already impossible to isolate pupils from the modern means of communication (smartphones, tablets, etc.. The objective of a teacher to show how you can use the gadgets for educational purposes. Interest in their use as a source of useful information. Learn to evaluate and select information sources on various criteria: reliability, availability, adequacy and completeness of the information, response rate, etc. Theseand other skills to work with information are key in our rapidly changing world, and are part of the concept of information culture of the modern man. This article describes the use of educational technology of QR-codes to address these issues, as well as justification its effectiveness. The intellectual quest, which is the embodiment of the above ideas, combines the dynamism and kindness, interest and attentiveness, ability to work in a team and contribute to the common cause; aimed at the development of tolerance, inquisitiveness, activity, courtesy, accuracy, attention and thinking; as well as to broaden their horizons and the formation of information culture of schoolboys.

  4. Religion as memory: How has the continuity of tradition produced collective meanings? – Part one

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    Jakub Urbaniak

    2015-06-01

    Full Text Available Danièle Hervieu-Léger gives an account of religion as a chain of memory, that is, a form of collective memory and imagination based on the sanctity of tradition. According to her theory, in the postmodern world the continuity of religious memory has been broken and all that remains are isolated fragments guarded by religious groups. This twofold study aims at showing, firstly, in what sense religion can be conceived of as memory which produces collective meanings (Part One and, secondly, what may happen when individualised and absolutised memories alienate themselves from a continuity of tradition, thus beginning to function as a sort of private religion (Part Two. Being the first part of the study in question, this article is dedicated to a historical-theological analysis of religious memory as a source of collective meanings, as seen from a Christian perspective. Firstly, it situates Hervieu-Léger’s definition of religion against the background of the most topical religious contexts in which the notion of memory appears today. Secondly, the dialectics of individual and collective memory is discussed, notably through the lens of Ricoeur’s original proposal. This is followed by an overview of the traditional functions of memory in Christianity. Lastly, the interpretation of the way in which Christian tradition, in its premodern continuity, served as a source of collective cultural meanings, is recapitulated. What underlies this analysis is the conviction that to comprehend, and even more so to challenge mechanisms based on which the dominant purveyors of meaning (such as economic and information market function in our day, one should have a clear understanding of what they attempt to substitute for. In brief, before exploring how memories become religion, one ought to be able to conceive of religion as memory.

  5. Domestically Produced TV-Drama and Cultural Commons

    DEFF Research Database (Denmark)

    From, Unni

    2005-01-01

    The Chapter elaborates empirically some of the aspects and means by which drama can occasion an articulation of cultural commons. Further, it explores explanations why audiences might retain a national perspective as a dominating frame of interpretation even though universal or late-modern interp......-modern interpretation frames would be equally relevant. I argue that institutional policy and politically regulations of media in general, combined with press material and a flow of news articles, chronicles, reviews, interviews and portraits , may establish a dominating paradigm....

  6. Cellular modifications produced by D2O in yeast culture

    International Nuclear Information System (INIS)

    Mihancea, I.; Mircea, R.Al.

    1996-01-01

    The cellular cycle of the Saccharomyces Cerevisiae, chosen as experimental object, is unmodified by the presence in the culture medium of D 2 O at different concentrations. An increased concentration of D 2 O in the culture medium leads to a decrease of the number of budded cells, to metabolic alterations, to DNA structure modification as well as to enzymatic changes produced by blocking. Other anomalies appear, as a function of the cell defence capacity and of the influence of the factors from the nutrient substrate or exterior medium. Due to D 2 O, the medium's pH changes and modifications at the enzyme level and of the cell microstructure and morphology occur. The enzymatic reactions take place in D 2 O slower than in H 2 O. Three-dimensional modifications appear in the organic components of the live cell which, in turn, produce profound modifications in the cell growth and division. Due to the kinetic and isotopic effects, modifications of the biochemical reactions affecting the cell integrity happen

  7. Sorry, We Didn't Mean to Break Your Culture

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    Lightfoot, R. C.

    Cultures meet and apparently insignificant traits or objects disrupt one of the cultures. This process repeatedly occurred in history with devastating consequences for the affected groups. Personal health, sanity, societies and cultures suffered from the effects of the new intrusions. Insights from sociological theory shed light on the effects and suggest remedies for at least some of the dysfunctions. Strategies for understanding and handling unforeseen changes must be prepared for future contact with other intelligences.

  8. Cross-Cultural Understanding for Global Sustainability: Messages and Meanings from Asian Cultural Landscapes

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    Singh, R.

    2013-11-01

    Concept of 'multifunctionality' of cultural landscapes is a reflection of imbued meaning and aesthetics inherent there and also human manifestation of this spirit through existence and aliveness by human creation, love and continuance in various cultures and traditions. This sense helps envisioning landscapes that cross urban-rural divides in sustainable and an integrated way - characterised by wholeness and ecospirituality that developed in the cultural history of landscape sustainability. That is how, the idea of 'wholeness' (cosmality) is transformed into 'holiness' (sacrality) ― evolved and represented with sacred ecology and visualised through the cosmic frames of sacredscapes in Asian region that survived there as part of lifeworld. Understanding, feeling, living with, practicing and passing on these inherent meanings and aesthetics provide peace, solace and deeper feelings to human mind which are the ethereal breathe of sustainability. The rethinking should be based on the foundational value ― the reasoning that underlies the ethical sense of deeper understanding of Man-Nature Interrelatedness, the basic philosophy of coexistence ― referred in different cultures in their own ways, like multicultural co-living ('Old-comer') in Korea, harmonious coexistence (tabunka kyosei) in Japan, harmonious society (xiaokang) in China, wahi tapu (sacred places) in Maori's New Zealand, global family (vasudhaiva kutumbakam) in Indian thought, and also African humanism (ubuntu) in South Africa. Think universally, see globally, behave regionally, act locally but insightfully; this is an appeal for shared wisdom for global sustainability in making our cultural landscapes mosaic of happy, peaceful and sustainable places crossing all the borders and transitions, especially interwoven links among Korea, Japan, China, and India.

  9. Understanding Cross-Cultural Meaning through Visual Media.

    Science.gov (United States)

    Hedberg, John G.; Brown, Ian

    2002-01-01

    Discusses cultural differences in Web site design for cross-cultural contexts and describes a study of Masters Degree students in Hong Kong that investigated their perceptions of Web learning environments that had been designed in Australia and delivered into Hong Kong and China. Considers the appropriateness of western interface design…

  10. Similarities and Differences in Meaning in Six Cultures.

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    Herrmann, Douglas J.; Raybeck, Douglas

    1981-01-01

    Employed multidimensional scaling to investigate semantic domains. Studied two semantic categories that varied in abstractness (animals and emotions) in six cultures (Greece, Haiti, Hong Kong, Spain, United States, and Vietnam). (Author/MK)

  11. Response to Richard Widdess: Music, Meaning and Culture

    Directory of Open Access Journals (Sweden)

    Jerome Lewis

    2012-09-01

    Full Text Available This commentary discusses the anthropological implications of Richard Widess’ paper by summarizing some anthropological approaches to music, especially focusing on the way musical participation inculcates and transmits an aesthetic orientation that guides action across cultural domains such as politics, economics and religion. The paper ends by suggesting that the heart of human culture is more likely to be an aesthetic orientation than a script or set of rules, and traces out some reasons why music does this so well.

  12. Teaching Students to Infer Meaning through Material Culture

    Science.gov (United States)

    Brewer, Ernest Andrew; Fritzer, Penelope

    2011-01-01

    Social studies students can learn to glean historical information from the study of material culture through active engagement as curators. Teachers can guide students through a pre-survey of helpful reading materials and then through selecting items of personal significance to them: creating labels that objectively describe the chosen items as to…

  13. Meanings and Implications of Culture in Sustainability Education Research

    Science.gov (United States)

    Anderson, Vince; Datta, Ranjan; Dyck, Shannon; Kayira, Jean; McVittie, Janet

    2016-01-01

    As scholars working both individually and collectively, we are interested in exploring what may be achieved through taking up the complex notion of culture in sustainability education research. In this article, we present a bricolage of research, drawing on empirical and theoretical sources that collectively establish the kind of capacity we see…

  14. Building a Strong Culture That Produces Sustainable Performance - 13444

    International Nuclear Information System (INIS)

    McDonald, John A. Jr

    2013-01-01

    Washington River Protection Solutions LLC (WRPS) has been involved with culture improvement for a number of years which has included co-chairing the industry effort to develop the EFCOG safety culture guidance documents [1, 2], and integration of this guidance into organizational processes and behavior expectations, described in more detail below. As various organizational cultural assessments have been periodically performed, and subsequent actions implemented to address improvement opportunities, organizational performance has shown improvement. Culture improvement is evident in the company's industrial safety statistics, event rates, safety culture survey results, employee morale, productivity, leadership effectiveness, and employee engagement. There does appear to be a relationship between striving to demonstrate behaviors consistent with excellent safety culture and good organizational performance over the past couple of years at WRPS. As performance continues to be evaluated, an improvement opportunity was identified to further enhance performance through field oriented behavioral/cultural improvement activities. WRPS recently conducted a three month effort to improve consistent implementation of management expectations by increasing management field presence with a focus on interacting real-time with workers and first line supervisors, and changing behaviors as appropriate. (authors)

  15. Building a Strong Culture That Produces Sustainable Performance - 13444

    Energy Technology Data Exchange (ETDEWEB)

    McDonald, John A. Jr [Washington River Protection Solutions LLC, PO Box 850, MSIN R2-53, Richland, WA 99352 (United States)

    2013-07-01

    Washington River Protection Solutions LLC (WRPS) has been involved with culture improvement for a number of years which has included co-chairing the industry effort to develop the EFCOG safety culture guidance documents [1, 2], and integration of this guidance into organizational processes and behavior expectations, described in more detail below. As various organizational cultural assessments have been periodically performed, and subsequent actions implemented to address improvement opportunities, organizational performance has shown improvement. Culture improvement is evident in the company's industrial safety statistics, event rates, safety culture survey results, employee morale, productivity, leadership effectiveness, and employee engagement. There does appear to be a relationship between striving to demonstrate behaviors consistent with excellent safety culture and good organizational performance over the past couple of years at WRPS. As performance continues to be evaluated, an improvement opportunity was identified to further enhance performance through field oriented behavioral/cultural improvement activities. WRPS recently conducted a three month effort to improve consistent implementation of management expectations by increasing management field presence with a focus on interacting real-time with workers and first line supervisors, and changing behaviors as appropriate. (authors)

  16. Teaching University Students Cultural Diversity by Means of Multi-Cultural Picture Books in Taiwan

    Science.gov (United States)

    Wu, Jia-Fen

    2017-01-01

    In a pluralistic society, learning about foreign cultures is an important goal in the kind of multi-cultural education that will lead to cultural competency. This study adopted a qualitative dominant mixed-method approach to examine the effectiveness of the multi-cultural picture books on: (1) students' achieving awareness towards cultural…

  17. Integrating cultural community psychology: activity settings and the shared meanings of intersubjectivity.

    Science.gov (United States)

    O'Donnell, Clifford R; Tharp, Roland G

    2012-03-01

    Cultural and community psychology share a common emphasis on context, yet their leading journals rarely cite each other's articles. Greater integration of the concepts of culture and community within and across their disciplines would enrich and facilitate the viability of cultural community psychology. The contextual theory of activity settings is proposed as one means to integrate the concepts of culture and community in cultural community psychology. Through shared activities, participants develop common experiences that affect their psychological being, including their cognitions, emotions, and behavioral development. The psychological result of these experiences is intersubjectivity. Culture is defined as the shared meanings that people develop through their common historic, linguistic, social, economic, and political experiences. The shared meanings of culture arise through the intersubjectivity developed in activity settings. Cultural community psychology presents formidable epistemological challenges, but overcoming these challenges could contribute to the transformation and advancement of community psychology.

  18. The meaning of work and cultural psychology: Ideas for new directions

    DEFF Research Database (Denmark)

    Bendassolli, Pedro; Tateo, Luca

    2018-01-01

    Work is one arena in which human beings constitute their identities and participate in collective-cultural enterprises. But research on factors affecting the meaning of work and its outcomes focuses mostly on individual-level variables related to workers’ experience. However, scholars have recently...... proposed a shift towards a more collective dimension of meaningfulness, in particular, the cultural level. This article discusses and expands on this recent trend, demonstrating how growing attention to cultural factors of work’s meaning raises some problematic, crucial issues about the very definition...... of culture and its role in meaning-making. A particular issue is the assumption that culture is transmitted to people, that it is primarily a collective endeavour based on shared values and that culture can endow work with meaning. Based on a cultural psychology perspective, we revisit both the relationship...

  19. Postcolonial constructions of HIV/AIDS: meaning, culture, and structure.

    Science.gov (United States)

    Sastry, Shaunak; Dutta, Mohan J

    2011-01-01

    As a field of inquiry, postcolonial health communication seeks to apprehend processes implicated in the construction of "primitive" versus "modern" with respect to issues of health. In the case of HIV/AIDS, the sociocultural representations of the disease have a profound impact on how the disease is configured medically and symbolically in dominant cultural imagination. Postcolonial constructions of disease are mobilized around the political and economic interests of the dominant power structures in global spaces. In this article, a thematic analysis of the constructions of HIV/AIDS in India in the mainstream U.S. news media was conducted. A corpus of news articles from the Lexis-Nexis database was created with the keywords "HIV," "AIDS," and "India." Three themes emerged from the study: (a) India as a site of biomedical control; (b) the economic logics of HIV/AIDS; and (c) AIDS, development, and the "Third World." Copyright © Taylor & Francis Group, LLC

  20. Emotions in “the world”: cultural practices, products, and meanings of anger and shame in two individualist cultures

    Science.gov (United States)

    Boiger, Michael; Deyne, Simon De; Mesquita, Batja

    2013-01-01

    Three studies tested the idea that people’s cultural worlds are structured in ways that promote and highlight emotions and emotional responses that are beneficial in achieving central goals in their culture. Based on the idea that U.S. Americans strive for competitive individualism, while (Dutch-speaking) Belgians favor a more egalitarian variant of individualism, we predicted that anger and shame, as well as their associated responses, would be beneficial to different extents in these two cultural contexts. A questionnaire study found that cultural practices promote beneficial emotions (anger in the United States, shame in Belgium) and avoid harmful emotions (shame in the United States): emotional interactions were perceived to occur more or less frequently to the extent that they elicited culturally beneficial or harmful emotions. Similarly, a cultural product analysis showed that popular children’s books from the United States and Belgium tend to portray culturally beneficial emotions more than culturally harmful emotions. Finally, a word-association study of the shared cultural meanings surrounding anger and shame provided commensurate evidence at the level of the associated response. In each language network, anger and shame were imbued with meanings that reflected the cultural significance of the emotion: while culturally consistent emotions carried relatively stronger connotations of emotional yielding (e.g., giving in to anger and aggressing against the offender in the United States), culturally inconsistent emotions carried relatively stronger connotations of emotional containment (e.g., a stronger emphasis on suppressing or transforming shame in the United States). PMID:24367340

  1. The cultural animal human nature, meaning, and social life

    CERN Document Server

    Baumeister, Roy F

    2005-01-01

    What makes us human? Why do people think, feel, and act as they do? What is the essence of human nature? What is the basic relationship between the individual and society? These questions have fascinated both great thinkers and ordinary humans for centuries. Now, at last, there is a solid basis for answering them, in the form of the accumulated efforts and studies by thousands of psychology researchers. We no longer have to rely on navel-gazing and speculation to understand why people are the way they are - we can instead turn to solid, objective findings. This book, by an eminent social psychologist at the peak of his career, not only summarizes what we know about people - it also offers a coherent, easy-to-understand, through radical, explanation. Turning conventional wisdom on its head, the author argues that culture shaped human evolution. Contrary to theories that depict the individual's relation to society as one of victimization, endless malleability, or just a square peg in a round hole, he proposes t...

  2. The implied producer investigating an emergent typology in participatory culture

    DEFF Research Database (Denmark)

    Søndergaard, Morten

    2012-01-01

    Biennales, that we are not 'just' dealing with a 'new' genre or style within the art category; on the other hand we are not dealing with a pure commercial culture either (the abstract notion of 'the user' has its limits); what is becoming evident is that the 'implicit' roles of the participatory 'actors...

  3. Cultural meanings of tuberculosis in Aceh Province, Sumatra.

    Science.gov (United States)

    Caprara, A; Abdulkadir, N; Idawani, C; Asmara, H; Lever, P; De Virgilio, G

    2000-07-01

    This paper shows how disease transmission and particularly what biomedicine calls tuberculosis are interpreted in the non-Western context of Aceh Province, Sumatra, Indonesia. It tries also to focus on factors influencing perceptions and health-seeking behaviors. Results show that what biomedicine calls TB is represented by a semantic network of illnesses. Parts of this network are clearly identified as transmissible while others are related to specific phenomena affecting the individual, such as terbuk (poisoning) or trouk (fatigue produced by hard work), and are not considered contagious. Forms of transmission are interpreted mostly through empirical and analogic categories. TB is attributed to four different aspects: (a) biomedical categories such as germ theory; (b) socio-economic conditions; (c) transgression of social rules; and (d) poisoning and the influences of supernatural powers. Health-seeking behaviors are related to the perceived causes of the disease, economic factors, and the accessibility of health services.

  4. Do Between-Culture Differences Really Mean that People Are Different? A Look at Some Measures of Culture Effect Size.

    Science.gov (United States)

    Matsumoto, David; Grissom, Robert J.; Dinnel, Dale L.

    2001-01-01

    Recommends four measures of cultural effect size appropriate for cross-cultural research (standardized difference between two sample means, probabilistic superiority effect size measure, Cohen's U1, and point biserial correlation), demonstrating their efficacy on two data sets from previously published studies and arguing for their use in future…

  5. Producing health, producing safety. Developing a collective safety culture in radiotherapy

    International Nuclear Information System (INIS)

    Nascimento, Adelaide

    2009-01-01

    This research thesis aims at a better understanding of safety management in radiotherapy and at proposing improvements for patient safety through the development of a collective safety culture. A first part presents the current context in France and abroad, addresses the transposition of other safety methods to the medical domain, and discusses the peculiarities of radiotherapy in terms of risks and the existing quality-assurance approaches. The second part presents the theoretical framework by commenting the intellectual evolution with respect to system safety and the emergence of the concept of safety culture, and by presenting the labour collective aspects and their relationship with system safety. The author then comments the variety of safety cultures among the different professions present in radiotherapy, highlights the importance of the collective dimension in correcting discrepancies at the end of the treatment process, and highlights how physicians take their colleagues work into account. Recommendations are made to improve patient safety in radiotherapy

  6. Microstructure and Textural Properties of Yoghurts Produced by Exopolysaccharides- Producing Starter Cultures

    DEFF Research Database (Denmark)

    Zhang, Lanjun

    conferred a ropy texture and resulted in yoghurts with decreased water holding capacity and an open microstructure. In addition, one of the LB strains with high amounts of EPS producing capacity improved water retention, when it was combined with an ST strain that produced negligible amounts of EPS...

  7. VISUAL CULTURE AS A MEANS OF FORMING A COMMON AND PROFESSIONAL CULTURE OF A PERSON

    Directory of Open Access Journals (Sweden)

    N. V. Syrova

    2018-01-01

    Full Text Available Introduction. The article is devoted to the analysis of the visual culture, which forms the general and professional culture of a person in many ways. Attention is paid to the culture of perception of visual images, the ability to analyze them, evaluate, compare, also the features of visual thinking and creative activity, directly relating to it, are considered. The mastering of visual culture is grounded by the specificity of various creative specialties and is a component of the educational process.Materials and Methods. The theory of personality formation and development is the methodological basis of the article. During the research, the analysis of the scientific and theoretical concept of "visual culture" in the system of general and professional human culture was used.Results. The article illustrates that the artistic vision, developed in the process of visual culture formation, starting from childhood, actively participates in the preparation of the ground for the origin of an artistic image, which is realized in the material later. With the development of the creative imagination of the learner, also the development of thinking and memory takes place inevitably, that is harmonization of all parts of the brain is carried out, and this process must be taken into account in the upbringing and education, the formation of the personality, and the formed visual culture is the basis for the improvement of all verges of the student's creative abilities.Discussion and Conclusions. The conclusion is made that visual culture being an integral part of the educational process is the necessary condition for entering the sociocultural space. The education of a high level of visual culture in a modern information and technically replete society is one of the necessary components of the formation of a general and professional culture of a person and it is desirable to begin this process with a student, and even better, from school-days.

  8. Meme Engineers: Children as Producers of Musical Culture

    Science.gov (United States)

    Barrett, Margaret

    2003-01-01

    This paper examines the ways in which young children construct and negotiate musical meaning as song-makers in a post-modern consumerist musical world. A critical analysis of the presentation of music in two popular Australian children's television programmes (Play School and Hi-5) and the ways in which such media impact upon children's…

  9. Determination of cosmic ray produced radionuclides by means of background radiation counting system, 3

    International Nuclear Information System (INIS)

    1976-01-01

    This is the third report of the progress report series on studies of cosmic ray produced radionuclides by means of low background radiation counting system. In Part I some characteristics of a low beta-gamma coincidence spectrometer are described -- counter system, electronics, background spectra, counting efficiencies -- and studies on radioactive impurities in materials for scientific research are also described. In Part II, suitable solvents for a large scale liquid scintillation counter were examined and best combinations of solvents, solutes and naphthalene are shown. In Part III, miscellaneous topics are reported. (auth.)

  10. High-Level Culturability of Epiphytic Bacteria and Frequency of Biosurfactant Producers on Leaves

    Science.gov (United States)

    Burch, Adrien Y.; Do, Paulina T.; Sbodio, Adrian; Suslow, Trevor V.

    2016-01-01

    ABSTRACT To better characterize the bacterial community members capable of biosurfactant production on leaves, we distinguished culturable biosurfactant-producing bacteria from nonproducers and used community sequencing to compare the composition of these distinct cultured populations with that from DNA directly recovered from leaves. Communities on spinach, romaine, and head lettuce leaves were compared with communities from adjacent samples of soil and irrigation source water. Soil communities were poorly described by culturing, with recovery of cultured representatives from only 21% of the prevalent operational taxonomic units (OTUs) (>0.2% reads) identified. The dominant biosurfactant producers cultured from soil included bacilli and pseudomonads. In contrast, the cultured communities from leaves are highly representative of the culture-independent communities, with over 85% of the prevalent OTUs recovered. The dominant taxa of surfactant producers from leaves were pseudomonads as well as members of the infrequently studied genus Chryseobacterium. The proportions of bacteria cultured from head lettuce and romaine leaves that produce biosurfactants were directly correlated with the culture-independent proportion of pseudomonads in a given sample, whereas spinach harbored a wider diversity of biosurfactant producers. A subset of the culturable bacteria in irrigation water also became enriched on romaine leaves that were irrigated overhead. Although our study was designed to identify surfactant producers on plants, we also provide evidence that most bacteria in some habitats, such as agronomic plant surfaces, are culturable, and these communities can be readily investigated and described by more classical culturing methods. IMPORTANCE The importance of biosurfactant production to the bacteria that live on waxy leaf surfaces as well as their ability to be accurately assessed using culture-based methodologies was determined by interrogating epiphytic populations by

  11. The Effect of Starter Culture Producing Exopolysaccharide on Physicochemical Properties of Yoghurt

    Directory of Open Access Journals (Sweden)

    Anatoli Cartasev

    2017-12-01

    Full Text Available The purpose of this research was to investigate the impact of indigenous starter culture capable to synthesize exopolysaccharides (EPSs on physicochemical properties of yoghurt. Two starter cultures, EPS-producing and non-EPS-producing, were developed from the autochthonous lactic acid bacteria strains by pairwise combining Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus strains. In the present study the ropy strain of Streptococcus thermophilus CNMN LB-50 was incorporated in EPS-producing starter culture. The microstructure, viscosity, EPS amount, structural properties and syneresis of yoghurt samples were assessed. It has been established that the EPS-producing starter culture provided a reduction of structural degradation and increased degree of structural recovery after deformation. Besides, it was observed that the use of EPS synthesized starter culture in yoghurt production restrains the syneresis of the gel.

  12. Biomass recycle as a means to improve the energy efficiency of CELSS algal culture systems

    Science.gov (United States)

    Radmer, R.; Cox, J.; Lieberman, D.; Behrens, P.; Arnett, K.

    1987-01-01

    Algal cultures can be very rapid and efficient means to generate biomass and regenerate the atmosphere for closed environmental life support systems. However, as in the case of most higher plants, a significant fraction of the biomass produced by most algae cannot be directly converted to a useful food product by standard food technology procedures. This waste biomass will serve as an energy drain on the overall system unless it can be efficiently recycled without a significant loss of its energy content. Experiments are reported in which cultures of the alga Scenedesmus obliquus were grown in the light and at the expense of an added carbon source, which either replaced or supplemented the actinic light. As part of these experiments, hydrolyzed waste biomass from these same algae were tested to determine whether the algae themselves could be made part of the biological recycling process. Results indicate that hydrolyzed algal (and plant) biomass can serve as carbon and energy sources for the growth of these algae, suggesting that the efficiency of the closed system could be significantly improved using this recycling process.

  13. HOW DOES THE MEANING THAT CONSUMERS DERIVE FROM ADVERTISEMENTS VARY ACROSS CULTURE?

    OpenAIRE

    Mohanasundaram, Arun Pragash

    2010-01-01

    Due to the convergence of homogenous consumer needs, taste and lifestyle, the globalization of media, incomes and technology were expected. But many researchers debated that adaptation is needed in the place of standardization in many places especially in the field of advertising. The aim of this dissertation is to analyze the influence of culture in advertising and also to show how does the meaning that consumers derive from advertisements, vary across cultures. The influence of culture in a...

  14. Training Culture: A New Conceptualization to Capture Values and Meanings of Training in Organizations

    Science.gov (United States)

    Polo, Federica; Cervai, Sara; Kantola, Jussi

    2018-01-01

    Purpose: The purpose of this study is to introduce and validate the concept of training culture defined as a subset of the main organizational culture that allows examining meanings and values attributed to the training within an organization by management and employees. Design/methodology/approach: This study, following the deductive scale…

  15. The Meaning of English Words across Cultures, with a Focus on Cameroon and Hong Kong

    Science.gov (United States)

    Bobda, Augustin Simo

    2009-01-01

    A word, even when considered monosemic, generally has a cluster of meanings, depending on the mental representation of the referent by the speaker/writer or listener/reader. The variation is even more noticeable across cultures. This paper investigates the different ways in which cultural knowledge helps in the interpretation of English lexical…

  16. 'I just said It, I didn't mean anything:' Culture and Pragmatic Inference ...

    African Journals Online (AJOL)

    'I just said It, I didn't mean anything:' Culture and Pragmatic Inference in Interpersonal Communication. Felix Nwabeze Ogoanah, Stella Nkechi Kpolugbo. Abstract. Socio-cultural practices and the economy of expression, which generally characterise human communication, significantly widen the gap between linguistic ...

  17. Overcoming the Discontinuity of Patriotic Education and Moral Development by Means of Regional Culture

    Science.gov (United States)

    Tikhonova, Anna U.

    2016-01-01

    The paper analyzes studies on continuing education, explains the concept of "continuity of patriotic education and moral development by means of regional culture", determines the uniqueness of regional culture as the core of patriotic education and moral development, and characterizes the model of continuity of patriotic education and…

  18. LEXICAL MEANING AND CULTURAL ADAPTATION ON THE PRODUCT OF NESTLE DANCOW

    Directory of Open Access Journals (Sweden)

    I Gusti Agung Istri Aryani

    2017-10-01

    Full Text Available Abstract Commonly, an amazing and attractive advertisement intends to hypnotize their target consumer in seeing, reading, or even hearing the ads continually. Besides, the power of persuading and motivating in messages from the way of informing product benefit could also give impact to buyer in deciding to buy the product. This research discusses the contexts of advertising found on packages of two Nestle products, especially Nestle Dancow Actigo and Nestle Dancow Enrich. These products were analyzed based on pictures or symbols and text found on packages. It is aimed at finding out the context of discourse advertising on those packages. Data were collected from analysis of text, including pictures or symbols and additional questionnaires distributed to 50 respondents located at Denpasar and Gianyar. Urban society were used to evaluate concerning on their understanding of English even though Indonesian language mostly applied on packages of products. Method used in analyzing data is descriptive qualitative and quantitative with simple statistics and explanation. It showed that connotation and denotation meanings highlighted lexical items and its cultural adaptation using process of copy adaptation to fit the culture of their targeted consumers. Means found 6 words or phrases of English from 10 of them on packages understood by 50 respondents as of: 35 for Full Cream as highest score and 16 respondents for FortiGro as the lowest score. In addition, brand name, symbol and images showed producer seemed to have close relationship with consumer and able to attract target   consumer attention as majority of findings. It can be concluded that ads on packages of Nestle Dancow had succeeded to be understood by the society through the messages implied.

  19. On some methods to produce high-energy polarized electron beams by means of proton synchrotrons

    International Nuclear Information System (INIS)

    Bessonov, E.G.; Vazdik, Ya.A.

    1980-01-01

    Some methods of production of high-energy polarized electron beams by means of proton synchrotrons are considered. These methods are based on transfer by protons of a part of their energy to the polarized electrons of a thin target placed inside the working volume of the synchrotron. It is suggested to use as a polarized electron target a magnetized crystalline iron in which proton channeling is realized, polarized atomic beams and the polarized plasma. It is shown that by this method one can produce polarized electron beams with energy approximately 100 GeV, energy spread +- 5 % and intensity approximately 10 7 electron/c, polarization approximately 30% and with intensity approximately 10 4 -10 5 electron/c, polarization approximately 100% [ru

  20. The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination.

    Science.gov (United States)

    Mu, Yang; Yang, Hou-Yun; Wang, Ya-Zhou; He, Chuan-Shu; Zhao, Quan-Bao; Wang, Yi; Yu, Han-Qing

    2014-06-10

    Fermentative hydrogen production from wastes has many advantages compared to various chemical methods. Methodology for characterizing the hydrogen-producing activity of anaerobic mixed cultures is essential for monitoring reactor operation in fermentative hydrogen production, however there is lack of such kind of standardized methodologies. In the present study, a new index, i.e., the maximum specific hydrogen-producing activity (SHAm) of anaerobic mixed cultures, was proposed, and consequently a reliable and simple method, named SHAm test, was developed to determine it. Furthermore, the influences of various parameters on the SHAm value determination of anaerobic mixed cultures were evaluated. Additionally, this SHAm assay was tested for different types of substrates and bacterial inocula. Our results demonstrate that this novel SHAm assay was a rapid, accurate and simple methodology for determining the hydrogen-producing activity of anaerobic mixed cultures. Thus, application of this approach is beneficial to establishing a stable anaerobic hydrogen-producing system.

  1. The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination

    Science.gov (United States)

    Mu, Yang; Yang, Hou-Yun; Wang, Ya-Zhou; He, Chuan-Shu; Zhao, Quan-Bao; Wang, Yi; Yu, Han-Qing

    2014-06-01

    Fermentative hydrogen production from wastes has many advantages compared to various chemical methods. Methodology for characterizing the hydrogen-producing activity of anaerobic mixed cultures is essential for monitoring reactor operation in fermentative hydrogen production, however there is lack of such kind of standardized methodologies. In the present study, a new index, i.e., the maximum specific hydrogen-producing activity (SHAm) of anaerobic mixed cultures, was proposed, and consequently a reliable and simple method, named SHAm test, was developed to determine it. Furthermore, the influences of various parameters on the SHAm value determination of anaerobic mixed cultures were evaluated. Additionally, this SHAm assay was tested for different types of substrates and bacterial inocula. Our results demonstrate that this novel SHAm assay was a rapid, accurate and simple methodology for determining the hydrogen-producing activity of anaerobic mixed cultures. Thus, application of this approach is beneficial to establishing a stable anaerobic hydrogen-producing system.

  2. Clusters of cultures: diversity in meaning of family value and gender role items across Europe.

    Science.gov (United States)

    van Vlimmeren, Eva; Moors, Guy B D; Gelissen, John P T M

    2017-01-01

    Survey data are often used to map cultural diversity by aggregating scores of attitude and value items across countries. However, this procedure only makes sense if the same concept is measured in all countries. In this study we argue that when (co)variances among sets of items are similar across countries, these countries share a common way of assigning meaning to the items. Clusters of cultures can then be observed by doing a cluster analysis on the (co)variance matrices of sets of related items. This study focuses on family values and gender role attitudes. We find four clusters of cultures that assign a distinct meaning to these items, especially in the case of gender roles. Some of these differences reflect response style behavior in the form of acquiescence. Adjusting for this style effect impacts on country comparisons hence demonstrating the usefulness of investigating the patterns of meaning given to sets of items prior to aggregating scores into cultural characteristics.

  3. A rapid radioassay for human IgG produced in lymphocyte in vitro culture systems

    International Nuclear Information System (INIS)

    Weightman, D.R.; Shale, D.J.; Tomlinson, W.R.

    1981-01-01

    Protein A bearing Staphylococcus aureus was used to develop a solid-phase radioassay for IgG immunoglobulins. The assay was specifically optimised for use in vitro human lymphocyte culture work. Compared with a solid-phase radioimmunoassay for IgG produced in lymphocyte culture, this assay had a similar performance profile and the advantages of rapidity and technical ease. (Auth.)

  4. Cybersemiotics: A suggestion for a transdisciplinary framework for description of observing, anticipatory and meaning producing systems

    Science.gov (United States)

    Brier, Soren

    1998-07-01

    The ability of systems to be anticipatory seems to be intricate connected with the ability to observe and to cognate by reducing complexity through signification. The semantic capacity of living systems, the cognitive ability to assign meaning to differences perturbating the system's self-organization, seems to be the prerequisite for the phenomenon of communication, language and consciousness. In cybernetics Bateson developed the idea that information is a difference that makes a difference and second order cybernetics developed the concept of organisms as self-organized and self-produced systems (autopoietic) as the prerequisite of life and cognition. The cognitive ability seems to be qualitative different from what so far is computable on any known machine although parts of different aspects of the process can be partly simulated in AI, neutral network and AL. In semiotics the fundamental process of cognition and communication is called semiosis or signification and C. S. Peirce created a special triadic, objective idealistic, pragmatic and evolutionary philosophy to be able to give a fruitful description of the process and its relation to logic and the concept of natural law. Both second order cybernetics and semiotics sees information and meaning as something produced by individual organisms through structural couplings to the environments or other individuals through historical drift and further developed in social communication. Luhmann points out that social communication also only functions through structural couplings which he calls generalized media such as science, art, power, love and money. Peirce talks of the semiotic net as a triadic view of meanings developing through history and in animals through evolution. In accordance with this Wittgenstein points out that signification is created in language games developed in specific life forms. Life forms are the things we do in society such as seducing, commanding and explaining. As animals do not have

  5. Crossing the cultural divide: issues in translation, mistrust, and cocreation of meaning in cross-cultural therapeutic assessment.

    Science.gov (United States)

    Rosenberg, Audrey; Almeida, Angelica; Macdonald, Heather

    2012-01-01

    This article examines cross-cultural therapeutic assessment in a community mental health clinic. The first case describes the work between a Caucasian assessor and a Mexican American family. The authors explore the metaphorical and literal translation of the findings from English to Spanish and the parallel process of translation of the self, experienced by both assessor and client. The second case describes the work between a Caucasian assessor and an African American adolescent. We describe the inherent challenge between the Eurocentric "task" orientation of the evaluation and the Afrocentric "relationship" orientation. We suggest that bridging the gap between cultures and overcoming cultural mistrust lay in the building of the assessor-client relationship. Fischer's concepts of rapport and intimacy are emphasized and expanded on as we emphasize the importance of cocreated meaning in cross-cultural assessment work.

  6. The new frontiers for CCT: Considering consumer-producer interaction in consumer culture

    NARCIS (Netherlands)

    MSc Lotte Salome

    2010-01-01

    Previous investigations of consumer subcultures in the CCT tradition focused primarily on consumer behaviours, feelings, experiences and meanings of consumption. This paper advocates that in order to deeply understand and interpret a particular subculture, researchers in consumer culture should

  7. Rudimentary, low tech incinerators as a means to produce reactive pozzolan out of sugar cane straw

    International Nuclear Information System (INIS)

    Martirena, Fernando; Middendorf, Bernhard; Day, Robert L.; Gehrke, Matthias; Roque, Pablo; Martinez, Lesday; Betancourt, Sergio

    2006-01-01

    The ashes of agricultural wastes from the processing of sugar cane are recognized as having pozzolanic properties. Burning of these wastes under controlled conditions, e.g. temperature and residence time results in significant improvement in reactivity. There are many reports of low-tech incinerators that have been successfully used to produce reactive rice husk ash in Asia. The paper presents the results of the evaluation of a rudimentary incinerator where sugar cane straw is burnt in order to obtain a reactive ash. The incinerator is designed and constructed according to state-of-the-art recommendations for this kind of device. Various burning trials were performed in order to obtain ash for the experiment. X-ray diffraction analysis performed on powdered ash shows significant presence of amorphous (glassy) material. Lime-pozzolana pastes were prepared. The pastes were subjected to X-ray diffraction, thermo-gravimetric analysis, chemical titration, and SEM observation, as a means to examine the pozzolanicity of the ash via the progress with time of calcium hydroxide consumption, and changes in the pore size distribution and strength. Calcium silicate hydrate phases are the main reaction product of the pozzolanic reaction. The long residence time of the ash in the burning chamber seems to be the reason for the fairly low reactivity of the ash; the reactivity of the ash was not significantly improved in comparison with that of the ash burnt in uncontrolled conditions in the open air

  8. Understanding asexual identity as a means to facilitate culturally competent care: A systematic literature review.

    Science.gov (United States)

    Jones, Catriona; Hayter, Mark; Jomeen, Julie

    2017-12-01

    To provide a contemporary overview of asexuality and the implications this has for healthcare practice. Individuals belonging to sexual minority groups face many barriers in accessing appropriate health care. The term "sexual minority group" is usually used to refer to lesbian women, gay, bisexual and transgender individuals. Anecdotal and research evidence suggests that those who identify as asexual have similar poor experiences. Systematic review and qualitative analysis. This work uses a systematic review and qualitative analysis of the existing interview data from self-identified asexuals, to construct features of the asexual identity. The findings will help practitioners and health professionals develop an understanding of this poorly understood construct. Ultimately this work is aimed at facilitating culturally competent care in the context of asexuality. Qualitative analysis produced three themes, which can be used, not only to frame asexuality in a positive and normalising way, but also to provide greater understanding of asexuality, "romantic differences coupled with sexual indifference," "validation through engagement with asexual communities" and "a diversity of subasexual identities." Having some understanding of what it means to identify as asexual, and respecting the choices made by asexuals can markedly improve the experiences of those who embrace an asexual identity when engaging with health care. Anecdotal evidence, taken from one of the largest asexual online forums, suggests that a number of self-identified asexuals choose not to disclose their identity to healthcare professionals through fear of their asexual status being pathologised, problematised or judged. Given that asexuality is a poorly understood concept, this may be due to lack of understanding on behalf of healthcare providers. The review provides health professionals and practitioners working in clinical settings with some insights of the features of an asexual identity to facilitate

  9. A strict anaerobic extreme thermophilic hydrogen-producing culture enriched from digested household waste

    DEFF Research Database (Denmark)

    Karakashev, Dimitar Borisov; Kotay, Shireen Meher; Trably, Eric

    2009-01-01

    The aim of this study was to enrich, characterize and identify strict anaerobic extreme thermophilic hydrogen (H-2) producers from digested household solid wastes. A strict anaerobic extreme thermophilic H-2 producing bacterial culture was enriched from a lab-scale digester treating household...... wastes at 70 degrees C. The enriched mixed culture consisted of two rod-shaped bacterial members growing at an optimal temperature of 80 degrees C and an optimal pH 8.1. The culture was able to utilize glucose, galactose, mannose, xylose, arabinose, maltose, sucrose, pyruvate and glycerol as carbon...... sources. Growth on glucose produced acetate, H-2 and carbon dioxide. Maximal H-2 production rate on glucose was 1.1 mmol l(-1) h(-1) with a maximum H-2 yield of 1.9 mole H-2 per mole glucose. 16S ribosomal DNA clone library analyses showed that the culture members were phylogenetically affiliated...

  10. How Do Cultural Producers Make Creative Decisions? Lessons from the Catwalk

    Science.gov (United States)

    Godart, Frederic C. Mears, Ashley

    2009-01-01

    Faced with high uncertainty, how do producers in the cultural economy make creative decisions? We present a case study of the fashion modeling industry. Using participant observation, interviews and network analysis of the Spring/Summer 2007 Fashion Week collections, we explain how producers select models for fashion shows. While fashion producers…

  11. Cross-Cultural Counseling and Cross-Cultural Meanings: An Exploration of Morita Psychotherapy.

    Science.gov (United States)

    Aldous, Jane L.

    1994-01-01

    Describes theoretical framework and techniques of Morita psychotherapy. Western research indicates that Asian American clients prefer active-directive, logical, rational, and structured approaches. Suggests that ethnocentric counseling approaches may be imposed upon clients of Asian origin because meanings attached to terms describing counseling…

  12. Children's activities and their meanings for parents: a mixed-methods study in six Western cultures.

    Science.gov (United States)

    Harkness, Sara; Zylicz, Piotr Olaf; Super, Charles M; Welles-Nyström, Barbara; Bermúdez, Moisés Ríos; Bonichini, Sabrina; Moscardino, Ughetta; Mavridis, Caroline Johnston

    2011-12-01

    Theoretical perspectives and research in sociology, anthropology, sociolinguistics, and cultural psychology converge in recognizing the significance of children's time spent in various activities, especially in the family context. Knowing how children's time is deployed, however, only gives us a partial answer to how children acquire competence; the other part must take into account the culturally constructed meanings of activities, from the perspective of those who organize and direct children's daily lives. In this article, we report on a study of children's routine daily activities and on the meanings that parents attribute to them in six Western middle-class cultural communities located in Italy, The Netherlands, Poland, Spain, Sweden, and the United States (N = 183). Using week-long time diaries kept by parents, we first demonstrate similarities as well as significant differences in children's daily routines across the cultural samples. We then present brief vignettes--"a day in the life" --of children from each sample. Parent interviews were coded for themes in the meanings attributed to various activities. Excerpts from parent interviews, focusing on four major activities (meals, family time, play, school- or developmentally related activities), are presented to illustrate how cultural meanings and themes are woven into parents' organization and understanding of their children's daily lives. The results of this mixed-method approach provide a more reliable and nuanced picture of children's and families' daily lives than could be derived from either method alone.

  13. Third Culture Kid's Effect on Character of Cady Heron in Mean Girls

    OpenAIRE

    Hapsari, Laily

    2017-01-01

    This thesis focuses on the movie, Mean Girls, specifically one of the character, Cady Heron,as a Third Culture Kid trying to adapt in new society. The purpose of this study is to understand the effect of Third Culture Kid in the movie character in socializing with the new surroundings and explain the way how one of the characters in Mean Girls adapts to the new surroundings.The writer uses library research to collect the data with the novel as the main data, and several relevant books, journa...

  14. Bishnuprasad Rabha as Cultural Icon of Assam: The Process of Meaning Making

    Directory of Open Access Journals (Sweden)

    Parismita Hazarika

    2017-03-01

    Full Text Available Abstract The term ‘cultural icon’ is generally used to refer to individuals or images, objects, visual sign, monuments, space etc. In semiotics the term ‘icon’ is used to refer to a sign that bears close resemblance to the object that it stands for. Icons are particularly influential signifiers because they are immediately identifiable and carry complex cultural codes in a compact image. In this paper the understanding of ‘cultural icon’ is not limited to semiotics. Following Keyan Tomaselli and David Scott in Cultural Icons (2009, we believe that cultural icons are purposive constructions. An attempt has been made in this paper to analyze the association of ‘desirable’ meanings to a cultural icon (while dropping ‘undesirable’ ones; thus, it is imperative that we look at the changing socio-political contexts behind such purposive constructions. With this in mind, we look at the iconic figure of Bishnuprasad Rabha who has been one of the most revered figures in the cultural history of Assam and has been appropriated as a cultural icon in different discourses of the national life of Assam that has emerged in recent times.

  15. Capacitors for Integrated Circuits Produced by Means of a Double Implantation Method

    International Nuclear Information System (INIS)

    Zukowski, P.; Partyka, J.; Wegierek, P.

    1998-01-01

    The paper presents a description of a method to produce capacitors in integrated circuits that consists in implanting weakly doped silicon with the same impurity, then subjecting it to annealing (producing the inner plate) and implanting it again with ions of neutral elements to produce the dielectric layer. Results of the testing capacitors produced that way are also presented. Unit capacity of C u = 4.5 nF/mm 2 at tgδ = 0.01 has been obtained. The authors are of the opinion that the interesting problem of discontinuous variations of dielectric losses and capacities considered as functions of temperature, must be viewed as an open problem. (author)

  16. Enhanced production and isotope enrichment of recombinant glycoproteins produced in cultured mammalian cells

    International Nuclear Information System (INIS)

    Skelton, David; Goodyear, Abbey; Ni, DaQun; Walton, Wendy J.; Rolle, Myron; Hare, Joan T.; Logan, Timothy M.

    2010-01-01

    NMR studies of post-translationally modified proteins are complicated by the lack of an efficient method to produce isotope enriched recombinant proteins in cultured mammalian cells. We show that reducing the glucose concentration and substituting glutamate for glutamine in serum-free medium increased cell viability while simultaneously increasing recombinant protein yield and the enrichment of non-essential amino acids compared to culture in unmodified, serum-free medium. Adding dichloroacetate, a pyruvate dehydrogenase kinase inhibitor, further improves cell viability, recombinant protein yield, and isotope enrichment. We demonstrate the method by producing partially enriched recombinant Thy1 glycoprotein from Lec1 Chinese hamster ovary (CHO) cells using U- 13 C-glucose and 15 N-glutamate as labeled precursors. This study suggests that uniformly 15 N, 13 C-labeled recombinant proteins may be produced in cultured mammalian cells starting from a mixture of labeled essential amino acids, glucose, and glutamate.

  17. Enhanced production and isotope enrichment of recombinant glycoproteins produced in cultured mammalian cells

    Energy Technology Data Exchange (ETDEWEB)

    Skelton, David; Goodyear, Abbey [Florida State University, Department of Chemistry and Biochemistry (United States); Ni, DaQun; Walton, Wendy J.; Rolle, Myron; Hare, Joan T. [Florida State University, Institute of Molecular Biophysics (United States); Logan, Timothy M., E-mail: tlogan@fsu.ed [Florida State University, Department of Chemistry and Biochemistry (United States)

    2010-10-15

    NMR studies of post-translationally modified proteins are complicated by the lack of an efficient method to produce isotope enriched recombinant proteins in cultured mammalian cells. We show that reducing the glucose concentration and substituting glutamate for glutamine in serum-free medium increased cell viability while simultaneously increasing recombinant protein yield and the enrichment of non-essential amino acids compared to culture in unmodified, serum-free medium. Adding dichloroacetate, a pyruvate dehydrogenase kinase inhibitor, further improves cell viability, recombinant protein yield, and isotope enrichment. We demonstrate the method by producing partially enriched recombinant Thy1 glycoprotein from Lec1 Chinese hamster ovary (CHO) cells using U-{sup 13}C-glucose and {sup 15}N-glutamate as labeled precursors. This study suggests that uniformly {sup 15}N,{sup 13}C-labeled recombinant proteins may be produced in cultured mammalian cells starting from a mixture of labeled essential amino acids, glucose, and glutamate.

  18. Three-dimensional neural cultures produce networks that mimic native brain activity.

    Science.gov (United States)

    Bourke, Justin L; Quigley, Anita F; Duchi, Serena; O'Connell, Cathal D; Crook, Jeremy M; Wallace, Gordon G; Cook, Mark J; Kapsa, Robert M I

    2018-02-01

    Development of brain function is critically dependent on neuronal networks organized through three dimensions. Culture of central nervous system neurons has traditionally been limited to two dimensions, restricting growth patterns and network formation to a single plane. Here, with the use of multichannel extracellular microelectrode arrays, we demonstrate that neurons cultured in a true three-dimensional environment recapitulate native neuronal network formation and produce functional outcomes more akin to in vivo neuronal network activity. Copyright © 2017 John Wiley & Sons, Ltd.

  19. Is the Appropriateness of Emotions Culture-Dependent? The Relevance of Social Meaning

    Directory of Open Access Journals (Sweden)

    Welpinghus Anna

    2018-02-01

    Full Text Available This paper contributes to the question to what extent the socio-cultural context is relevant for the appropriateness of emotions, while appropriateness of an emotion means that the emotion entails a correct, or adequate, evaluation of its object. In a first step, two adequacy conditions for theories of emotions are developed: the first condition ensures that the socio-cultural context is not neglected: theories must allow for the fact that appropriateness often depends on the social meaning of the emotion’s particular object. The second condition rules out implausible forms of cultural determinism. In a second step, an account that meets both conditions is presented: the Acceptance within Social Context account.

  20. Of Models and Meanings: Cultural Resilience in Social-Ecological Systems

    Directory of Open Access Journals (Sweden)

    Todd A. Crane

    2010-12-01

    Full Text Available Modeling has emerged as a key technology in analysis of social-ecological systems. However, the tendency for modeling to focus on the mechanistic materiality of biophysical systems obscures the diversity of performative social behaviors and normative cultural positions of actors within the modeled system. The fact that changes in the biophysical system can be culturally constructed in different ways means that the perception and pursuit of adaptive pathways can be highly variable. Furthermore, the adoption of biophysically resilient livelihoods can occur under conditions that are subjectively experienced as the radical transformation of cultural systems. The objectives of this work are to: (1 highlight the importance of understanding the place of culture within social-ecological systems, (2 explore the tensions between empirical and normative positions in the analysis of social-ecological resilience, and (3 suggest how empirical modeling of social-ecological systems can synergistically interact with normative aspects of livelihoods and lifeways.

  1. Cultural Interpretations of the Visual Meaning of Icons and Images Used in North American Web Design

    Science.gov (United States)

    Knight, Eliot; Gunawardena, Charlotte N.; Aydin, Cengiz Hakan

    2009-01-01

    This study examines cross-cultural interpretations of icons and images drawn from US academic websites. Participants from Morocco, Sri Lanka, Turkey, and the USA responded to an online questionnaire containing 18 icons and images representing online functions and information types common on US academic websites. Participants supplied meanings for…

  2. Mutual expectation compacts: a means to link practice culture and vision.

    Science.gov (United States)

    Drury, Ivo; Slomski, Carol

    2007-01-01

    We describe the development of a mutual expectation compact in an academic department of surgery as a means of reinforcing the department's vision document and promoting cultural change. The compact makes explicit those implicit agreements that exist between a physician and his or her practice. It strengthens the relationship by avoiding the misunderstandings that can arise when agreements are implicit.

  3. Formation of Pedagogical System for Individual Self-Development by Means of Physical Culture and Sport

    Science.gov (United States)

    Panachev, Valery D.; Zelenin, Leonid A.; Opletin, Anatoly A.; Verbytskyi, Sergei A.

    2017-01-01

    Problems of formation, development and introduction of the modern pedagogical selfdevelopment system in university educational process by means of physical culture and sport have been considered in this article. Such generated pedagogical system reflects practical implementation of social order on the modern educational paradigm aimed at creation…

  4. An Empirical Model and Ethnic Differences in Cultural Meanings Via Motives for Suicide.

    Science.gov (United States)

    Chu, Joyce; Khoury, Oula; Ma, Johnson; Bahn, Francesca; Bongar, Bruce; Goldblum, Peter

    2017-10-01

    The importance of cultural meanings via motives for suicide - what is considered acceptable to motivate suicide - has been advocated as a key step in understanding and preventing development of suicidal behaviors. There have been limited systematic empirical attempts to establish different cultural motives ascribed to suicide across ethnic groups. We used a mixed methods approach and grounded theory methodology to guide the analysis of qualitative data querying for meanings via motives for suicide among 232 Caucasians, Asian Americans, and Latino/a Americans with a history of suicide attempts, ideation, intent, or plan. We used subsequent logistic regression analyses to examine ethnic differences in suicide motive themes. This inductive approach of generating theory from data yielded an empirical model of 6 cultural meanings via motives for suicide themes: intrapersonal perceptions, intrapersonal emotions, intrapersonal behavior, interpersonal, mental health/medical, and external environment. Logistic regressions showed ethnic differences in intrapersonal perceptions (low endorsement by Latino/a Americans) and external environment (high endorsement by Latino/a Americans) categories. Results advance suicide research and practice by establishing 6 empirically based cultural motives for suicide themes that may represent a key intermediary step in the pathway toward suicidal behaviors. Clinicians can use these suicide meanings via motives to guide their assessment and determination of suicide risk. Emphasis on environmental stressors rather than negative perceptions like hopelessness should be considered with Latino/a clients. © 2017 Wiley Periodicals, Inc.

  5. SOCIO-CULTURAL COMPETENCE FORMATION BY MEANS OF TRANSLATION IN THE CONDITIONS OF INCLUSIVE EDUCATION

    Directory of Open Access Journals (Sweden)

    Kateryna Shapochka

    2016-12-01

    Full Text Available The article is devoted to the problem of socio-cultural competence formation by means of translation / interpretation and the necessity of foreign language communicative competence formation in the process of inclusive education. The question of training of young generation for life in a multi-ethnic and multicultural society, forming skills of communication and cooperation with people of different nationalities, the foreign language learning, the formation of the communicative and socio-cultural competence is one of the main tasks of modern school to meet educational needs persons with disabilities. Today’s realities require that students with special educational needs should study a foreign language and use it in the process of learning. In turn, the use of translation in the process of learning a foreign language helps students to get new skills, to form general and specific competences, including socio-cultural competence, which promotes socialization of children with special needs, and integrating them into a comprehensive system of Ukraine. The article raises the problem of modernization of the educational system. It was established that the formation of socio-cultural competence by means of written translation is done by means of a system of exercises. Based on this system, subsystems, groups and types of exercises their systems can be developed in accordance with human activity, objectives and learning environment. It shows that the development of an inclusive approach to learning demands new solutions towards learning a foreign language at different levels of education.

  6. [Diversity and enzyme-producing activity of culturable halophilic bacteria in Daishan Saltern of East China].

    Science.gov (United States)

    Yang, Dan-Dan; Li, Qian; Huang, Jing-Jing; Chen, Min

    2012-11-01

    Soil and saline water samples were collected from the Daishan Saltern of East China, and the halophilic bacteria were isolated and cultured by using selective media, aimed to investigate the diversity and enzyme-producing activity of culturable halophilic bacteria in saltern environment. A total of 181 strains were isolated by culture-dependent method. Specific primers were used to amplify the 16S rRNA gene of bacteria and archaea. The operation taxonomy units (OTUs) were determined by ARDRA method, and the representative strain of each OTU was sequenced. The phylogenetic position of all the isolated strains was determined by 16S rRNA sequencing. The results showed that the isolated 181 strains displayed 21 operational taxonomic units (OTUs), of which, 12 OTUs belonged to halophilic bacteria, and the others belonged to halophilic archaea. Phylogenetic analysis indicated that there were 7 genera presented among the halophilic bacteria group, and 4 genera presented among the halophilic archaea group. The dominant halophilic strains were of Halomonas and Haloarcula, with 46.8% in halophilic bacteria and 49.1% in halophilic archaea group, respectively. Enzyme-producing analysis indicated that most strains displayed enzyme-producing activity, including the activities of producing amylase, proteinase and lipase, and the dominant strains capable of enzyme-producing were of Haloarcula. Our results showed that in the environment of Daishan Saltern, there existed a higher diversity of halophilic bacteria, being a source sink for screening enzyme-producing bacterial strains.

  7. Resveratrol Produces Neurotrophic Effects on Cultured Dopaminergic Neurons through Prompting Astroglial BDNF and GDNF Release

    Directory of Open Access Journals (Sweden)

    Feng Zhang

    2012-01-01

    Full Text Available Increasing evidence indicated astroglia-derived neurotrophic factors generation might hold a promising therapy for Parkinson’s disease (PD. Resveratrol, naturally present in red wine and grapes with potential benefit for health, is well known to possess a number of pharmacological activities. Besides the antineuroinflammatory properties, we hypothesized the neuroprotective potency of resveratrol is partially due to its additional neurotrophic effects. Here, primary rat midbrain neuron-glia cultures were applied to investigate the neurotrophic effects mediated by resveratrol on dopamine (DA neurons and further explore the role of neurotrophic factors in its actions. Results showed resveratrol produced neurotrophic effects on cultured DA neurons. Additionally, astroglia-derived neurotrophic factors release was responsible for resveratrol-mediated neurotrophic properties as evidenced by the following observations: (1 resveratrol failed to exert neurotrophic effects on DA neurons in the cultures without astroglia; (2 the astroglia-conditioned medium prepared from astroglia-enriched cultures treated with resveratrol produced neurotrophic effects in neuron-enriched cultures; (3 resveratrol increased neurotrophic factors release in the concentration- and time-dependent manners; (4 resveratrol-mediated neurotrophic effects were suppressed by blocking the action of the neurotrophic factors. Together, resveratrol could produce neurotrophic effects on DA neurons through prompting neurotrophic factors release, and these effects might open new alternative avenues for neurotrophic factor-based therapy targeting PD.

  8. Meaning

    Science.gov (United States)

    Harteveld, Casper

    The second world to be considered concerns Meaning. In contrast to Reality and Play, this world relates to the people, disciplines, and domains that are focused on creating a certain value. For example, if this value is about providing students knowledge about physics, it involves teachers, the learning sciences, and the domains education and physics. This level goes into the aspects and criteria that designers need to take into account from this perspective. The first aspect seems obvious when we talk of “games with a serious purpose.” They have a purpose and this needs to be elaborated on, for example in terms of what “learning objectives” it attempts to achieve. The subsequent aspect is not about what is being pursued but how. To attain a value, designers have to think about a strategy that they employ. In my case this concerned looking at the learning paradigms that have come into existence in the past century and see what they have to tell us about learning. This way, their principles can be translated into a game environment. This translation involves making the strategy concrete. Or, in other words, operationalizing the plan. This is the third aspect. In this level, I will further specifically explain how I derived requirements from each of the learning paradigms, like reflection and exploration, and how they can possibly be related to games. The fourth and final aspect is the context in which the game is going to be used. It matters who uses the game and when, where, and how the game is going to be used. When designers have looked at these aspects, they have developed a “value proposal” and the worth of it may be judged by criteria, like motivation, relevance, and transfer. But before I get to this, I first go into how we human beings are meaning creators and what role assumptions, knowledge, and ambiguity have in this. I will illustrate this with some silly jokes about doctors and Mickey Mouse, and with an illusion.

  9. Lipolytic Changes in Fermented Sausages Produced with Turkey Meat: Effects of Starter Culture and Heat Treatment

    OpenAIRE

    Karslo?lu, Bet?l; ?i?ek, ?mran Ensoy; Kolsarici, Nuray; Cando?an, Kezban

    2014-01-01

    In this study, the effects of two different commercial starter culture mixes and processing methodologies (traditional and heat process) on the lipolytic changes of fermented sausages manufactured with turkey meat were evaluated during processing stages and storage. Free fatty acid (FFA) value increased with fermentation and during storage over 120 d in all fermented sausage groups produced with both processing methodologies (p

  10. Culture care meanings and experiences of postpartum depression among Jordanian Australian women: a transcultural study.

    Science.gov (United States)

    Nahas, V; Amasheh, N

    1999-01-01

    This study discovers, describes, and explains the personal experiences, perceptions, and care meanings of Jordanian women who have suffered postpartum depression. Most postpartum cases often are misdiagnosed as exclusively psychological and untreated by health care professionals without consideration to the cultural meanings of this problem. Understanding the experiences of these women is important, as their expressions often are contextually and culturally influenced. Using Leininger's Theory of Culture Care Diversity and Universality, a purposive sample of 22 Jordanian women diagnosed with postpartum depression, living in Sydney, were interviewed. The ethnonursing research method and data analysis procedures were used. Results revealed that Jordanian mothers experienced severe loss of control over emotions of loneliness, hopelessness, and feelings of being a bad mother. Three major themes focusing on the care meanings and experiences of Jordanian women are discussed: (a) Care means strong family support and kinship during the postpartum period, (b) care is carrying out and fulfilling traditional gender roles as mother and wife, and (c) care is preservation of Jordanian childbearing customs as expressed in the celebration of the birth of the baby.

  11. Material Culture and Cultural Meanings: Current Studies of Archaeological Ceramics and in Ce­ramic Ethnoarchaeology

    Directory of Open Access Journals (Sweden)

    Charles C. Kolb

    2000-05-01

    Full Text Available Pottery and People: A Dynamic Interaction. James M. Skibo and Gary M. Feinman (editors. Founda­tions of Archaeological Inquiry. Salt Lake City. University of Utah Press. 1999. 260 pp. 91 figures. 30 tables. ISBN 0-87480-576-7. $55.00 (cloth; 0-87489-577-5. $25.00 (paperback. Material Meanings: Critical Approaches to the Interpretation of Material Culture. Elizabeth S. Chilton (editor. Foundations of Archaeological Inquiry. Salt Lake City: University of Utah Press. 1999.179pp. 35 figures. 11 tables. ISBN 0-87480-632-1. $55.00 (cloth; 087480-633-X. $35.00 (paperback. The description and interpretation of material culture may be regarded as the essence of archaeology, a discipline that seeks to recover, describe, document, and interpret past human culture. More recently, understanding that actions occur in a material world that is constituted symbolically, archaeological explanations are often framed in sociocultural meanings, the analysis of agencies, practices and behaviors. I shall subsequently return to this issue. Because of their longevity in the archaeological record, lithic and ceramic artifacts are crucial to the endeavor to interpret human culture. Objects fashioned from clay and subjected to intentional artificial sources of heat made their initial appearance in the archaeological record more than 26.000 years ago. Ceramic objects have been created in a seemingly endless variety of shapes and forms, varying from fertility figurines. to cooking and food storage vessels. lamps, smoking pipes, medicinal pastilles, tokens, beehives, and coffins to modern whitewares and pyroceramics.

  12. Distribution of binder in granules produced by means of twin screw granulation

    DEFF Research Database (Denmark)

    Fonteyne, Margot; Fussell, Andrew Luke; Vercruysse, Jurgen

    2014-01-01

    According to the quality by design principle processes may not remain black-boxes and full process understanding is required. The granule size distribution of granules produced via twin screw granulation is often found to be bimodal. The aim of this study was to gain a better understanding...

  13. Relationships between food producers and retail chains seen as shared meanings

    DEFF Research Database (Denmark)

    Skytte, Hans

    This paper presents a new theory on relationships between producers and retail chains. This theory is a result of a project which investigated the cooperation between Danish abattoirs and food processors, and retail chains in four countries. The new theory's main point is that relationships betwe...

  14. Developing Psychological Culture of Schoolchildren as a Means of Supporting Implementation of Basic Education Standards

    Directory of Open Access Journals (Sweden)

    Dubrovina I.V.

    2018-01-01

    Full Text Available The paper reviews the social situation of development of children and adolescents in the modern society marked by rapid changes. The development of children and adolescents is described as ‘embedding into the culture’ through the education and is closely associated with the formation of their psychological culture. The paper analyses the conditions of personality development in modern children and adolescents, the factors which impede the communication and understanding of other people; it highlights the risks of escaping into the virtual reality or joining asocial groups. The paper also suggests important measures aimed at the formation of psychological culture in children in relation to age-specific tasks of development in primary school and adolescent ages. The development of psychological culture is regarded as the key means of supporting the implementation of modern educational standards as well as the foundation of psychological health in schoolchildren.

  15. Finding meaning in first episode psychosis: experience, agency, and the cultural repertoire.

    Science.gov (United States)

    Larsen, John Aggergaard

    2004-12-01

    The article examines individuals' attempts to generate meaning following their experiences with psychosis. The inquiry is based on a person-centered ethnographic study of a Danish mental health community program for early intervention in schizophrenia and involves longitudinal interviews with 15 of its participants. The article takes an existential anthropological perspective emphasizing agency and cultural phenomenology to investigate how individuals draw on resources from the cultural repertoire to make sense of personally disturbing experiences during their psychosis. It is suggested that the concept of "system of explanation" has advantages over, for example, "illness narrative" and "explanatory model" when demonstrating how some individuals engage in the creative analytic and theory-building work of bricolage, selecting, adding, and combining various systems of explanation. Delusions are equally derived from the cultural repertoire but are constructed as dogmatic explanations that are idiosyncratic to the individual who holds them.

  16. The Cultural Voice of Immigrant Latina Women and the Meaning of Femininity

    Directory of Open Access Journals (Sweden)

    Ellen Greer

    2013-04-01

    Full Text Available This phenomenological study investigates the cultural meaning of femininity to immigrant Latina women and its significance in the consideration of decisions related to maintaining breast health. Theories of culture and health promotion support the concern for women’s need for access to health care in relation to breast health and the cultural barriers that interface between the connections of femininity, body image, and mental health. For Hispanic women, breast cancer is the most commonly diagnosed cancer generating distress for the individual, partner, and family. The importance of best practices in health promotion and risk reduction strategies in the early screening of women is emphasized in the literature. However, the voice of immigrant Latina women in describing their perspective of femininity and breast health and how it may affect choice and decision making related to breast self-care practices has not been studied. Five themes emerged from the data analysis conducted with Giorgi’s phenomenological method leading to an unfolded description of femininity: power of feminine identity through motherhood, hardiness is sustainability to overcoming adversity, connection to self and others, satisfaction meeting cultural gender-role expectations, and contemplative prevention to maintain breast integrity. The findings of this study will contribute to the increasing body of evidence-based practice related to understanding the impact of culture related to breast health. While the concept of femininity can be challenged not only by a diagnosis of breast cancer but also by other diseases and life conditions, understanding the cultural meaning of femininity to Latina women is pivotal to health care professionals as they partner with Latina women and community support groups to develop empowerment strategies and programs that promote choice and decision making involving breast health.

  17. Symbolic Meanings of High and Low Impact Daily Consumption Practices in Different Cultures

    DEFF Research Database (Denmark)

    Ger, Güliz; Wilhite, Harold; Halkier, Bente

    1998-01-01

    . They influence our choices and practices whether it is by motivating or impeding them. In this study we will look at the symbolic meanings of high and low environmental impact consumption practices. In order to create desirable social markers for environmentally-friendly practices and/or to change high impact...... practices, we need to understand emergent practices and their existing cultural meanings. Thus we have chosen three fields of daily consumption practices - food consumption, transport and hygiene - and sorted out the relatively environmentally friendly (low impact) and the relatively environmentally...

  18. Ammonia produced by bacterial colonies promotes growth of ampicillin-sensitive Serratia sp. by means of antibiotic inactivation.

    Science.gov (United States)

    Cepl, Jaroslav; Blahůšková, Anna; Cvrčková, Fatima; Markoš, Anton

    2014-05-01

    Volatiles produced by bacterial cultures are known to induce regulatory and metabolic alterations in nearby con-specific or heterospecific bacteria, resulting in phenotypic changes including acquisition of antibiotic resistance. We observed unhindered growth of ampicillin-sensitive Serratia rubidaea and S. marcescens on ampicillin-containing media, when exposed to volatiles produced by dense bacterial growth. However, this phenomenon appeared to result from pH increase in the medium caused by bacterial volatiles rather than alterations in the properties of the bacterial cultures, as alkalization of ampicillin-containing culture media to pH 8.5 by ammonia or Tris exhibited the same effects, while pretreatment of bacterial cultures under the same conditions prior to antibiotic exposure did not increase ampicillin resistance. Ampicillin was readily inactivated at pH 8.5, suggesting that observed bacterial growth results from metabolic alteration of the medium, rather than an active change in the target bacterial population (i.e. induction of resistance or tolerance). However, even such seemingly simple mechanism may provide a biologically meaningful basis for protection against antibiotics in microbial communities growing on semi-solid media. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  19. Increasing the production of desulfurizing biocatalysts by means of fed - batch culture

    International Nuclear Information System (INIS)

    Berdugo, C I; Mena, J A; Acero, J R; Mogollon, L

    2001-01-01

    Over the past years, environmental regulations have driven a lot of effort for the development of new technologies for the upgrading of fossil fuels. Biotechnology offers an alternative way to process fossil fuels by means of a biodesulfurization technology where the production of the biocatalyst is one of the key topics. Traditionally, the production is carried out in batch culture where the maximum cellular concentration is restricted by inherent limitations of the culture type and the microorganism growth rate. This work addresses the production of two desulfurizing microorganisms: Rhodococcus erythropolis IGTS8 and gordona rubropertinctus ICP172 using fed-batch culture. Fed-batch cultures were conducted in a 12 L fermentor using ICP 4 medium containing glucose and DMSO as carbon and sulfur sources. As a result, cell concentration was increased 1.5 and 3 times with fed-batch cultures using constant and exponential flow respectively, achieving a maximum cell concentration of 7.3 g DCW/L of biocatalyst igts8 and 12.85 gGDCW/L of the new biocatalyst ICP172. Both biocatalysts presented biodesulfurization activity in a spiked matrix DBT/HXD and in diesel matrix with the detection of 2-HBP which is the end-product of DBT degradation pathway

  20. Gas and Pressure Dependence for the Mean Size of Nanoparticles Produced by Laser Ablation of Flowing Aerosols

    International Nuclear Information System (INIS)

    Nichols, William T.; Malyavanatham, Gokul; Henneke, Dale E.; Brock, James R.; Becker, Michael F.; Keto, John W.; Glicksman, Howard D.

    2000-01-01

    Silver nanoparticles were produced by laser ablation of a continuously flowing aerosol of microparticles entrained in argon, nitrogen and helium at a variety of gas pressures. Nanoparticles produced in this new, high-volume nanoparticle production technique are compared with our earlier experiments using laser ablation of static microparticles. Transmission electron micrographs of the samples show the nanoparticles to be spherical and highly non-agglomerated under all conditions tested. These micrographs were analyzed to determine the effect of carrier gas type and pressure on size distributions. We conclude that mean diameters can be controlled from 4 to 20 nm by the choice of gas type and pressure. The smallest nanoparticles were produced in helium, with mean sizes increasing with increasing molecular weight of the carrier gas. These results are discussed in terms of a model based on cooling via collisional interaction of the nanoparticles, produced in the laser exploded microparticle, with the ambient gas

  1. The conducting shell stellarator: A simple means for producing complicated fields

    International Nuclear Information System (INIS)

    Sheffield, G.V.

    1997-01-01

    One of the main characteristics of stellarators, both helical and modular, is that their coil sets must take difficult shapes in order to produce the complicated stellarator magnetic fields. The complex coil shapes make fabrication difficult and costly compared to say the toroidal field, TF, coil set of a tokamak. The conducting shell stellarator, CSS, configuration described in this report shows that complicated stellarator fields can be produced by inducing eddy currents in a conducting shell from a simple TF coil set (a field that varies like 1/R). This technique is applicable not only to a pulsed system at room or cryogenic temperatures, but can be implemented for a superconducting TF with a superconducting shell in a stellarator reactor. The CSS has the added benefit that within this device the metallic shell which can be made up of discrete plates can be changed out and replaced with new plates to create a different stellarator configuration within the same TF coil set. The work of creating the complicated magnetics is done by the passive conductor reshaping the simple TF field

  2. Producing intricate IPMC shapes by means of spray-painting and printing (Conference Presentation)

    Science.gov (United States)

    Trabia, Sarah; Olsen, Zakai; Hwang, Taeseon; Kim, Kwang Jin

    2017-04-01

    Ionic Polymer-Metal Composites (IPMC) are common soft actuators that are Nafion® based and plated with a conductive metal, such as platinum, gold, or palladium. Nafion® is available in three forms: sheets, pellets, and water dispersion. Nafion® sheets can be cut to the desired dimensions and are best for rectangular IPMCs. However, the user is not able to change the thickness of these sheets by stacking and melting because Nafion® does not melt. A solution to this is Nafion® pellets, which can melt. These can be used for extrusion and injection molding. Though Nafion® pellets can be melted, they are difficult to work with, making the process quite challenging to master. The last form is Nafion® Water Dispersion, which can be used for casting. Casting can produce the desired thickness, but it does not solve the problem of achieving complex contours. The current methods of fabrication do not allow for complex shapes and structures. To solve this problem, two methods are presented: painting and printing. The painting method uses Nafion® Water Dispersion, an airbrush, and vinyl stencils. The stencils can be made into any shape with detailed edges. The printing method uses Nafion® pellets that are extruded into filaments and a commercially available 3D printer. The models are drawn in a Computer-Aided Drawing (CAD) program, such as SolidWorks. The produced Nafion® membranes will be compared with a commercial Nafion® membrane through a variety of tests, including Fourier Transform Infrared Spectroscopy, Scanning Electron Microscope, Thermogravimetric Analysis, Dynamic Mechanical Analysis, and Optical Microscope.

  3. Effect of airstream velocity on mean drop diameters of water sprays produced by pressure and air atomizing nozzles

    Science.gov (United States)

    Ingebo, R. D.

    1977-01-01

    A scanning radiometer was used to determine the effect of airstream velocity on the mean drop diameter of water sprays produced by pressure atomizing and air atomizing fuel nozzles used in previous combustion studies. Increasing airstream velocity from 23 to 53.4 meters per second reduced the Sauter mean diameter by approximately 50 percent with both types of fuel nozzles. The use of a sonic cup attached to the tip of an air assist nozzle reduced the Sauter mean diameter by approximately 40 percent. Test conditions included airstream velocities of 23 to 53.4 meters per second at 293 K and atmospheric pressure.

  4. The determination of the hadron mean free path for particle-producing collisions in intranuclear matter by measurement

    International Nuclear Information System (INIS)

    Strugalski, Z.; Mousa, M.

    1987-01-01

    It is shown how it is possible to determine the hadron mean free path in > for particle-producing collisions in intranuclear matter by measurement. The mean free path for the collisions of pions inside 54 131 Xe nuclei at 3.5 GeV/c momentum has been measured. The relation between in > in units nucleons/S and the hadron-nucleon inelastic cross section σ in in units S/nucleon is found: in >k1/σ in , where S∼10 fm 2 , k=3.0±0.15; physical meaning of S is given in this paper

  5. Culture and Palliative Care: Preferences, Communication, Meaning, and Mutual Decision Making.

    Science.gov (United States)

    Cain, Cindy L; Surbone, Antonella; Elk, Ronit; Kagawa-Singer, Marjorie

    2018-05-01

    Palliative care is gaining acceptance across the world. However, even when palliative care resources exist, both the delivery and distribution of services too often are neither equitably nor acceptably provided to diverse population groups. The goal of this study was to illustrate tensions in the delivery of palliative care for diverse patient populations to help clinicians to improve care for all. We begin by defining and differentiating culture, race, and ethnicity, so that these terms-often used interchangeably-are not conflated and are more effectively used in caring for diverse populations. We then present examples from an integrative literature review of recent research on culture and palliative care to illustrate both how and why varied responses to pain and suffering occur in different patterns, focusing on four areas of palliative care: the formation of care preferences, communication patterns, different meanings of suffering, and decision-making processes about care. For each area, we provide international and multiethnic examples of variations that emphasize the need for personalization of care and the avoidance of stereotyping beliefs and practices without considering individual circumstances and life histories. We conclude with recommendations for improving palliative care research and practice with cultural perspectives, emphasizing the need to work in partnerships with patients, their family members, and communities to identify and negotiate culturally meaningful care, promote quality of life, and ensure the highest quality palliative care for all, both domestically and internationally. Copyright © 2018 American Academy of Hospice and Palliative Medicine. Published by Elsevier Inc. All rights reserved.

  6. Meaning in hoarding: perspectives of people who hoard on clutter, culture and agency.

    Science.gov (United States)

    Orr, David M R; Preston-Shoot, Michael; Braye, Suzy

    2017-12-12

    Hoarding has become increasingly prominent in clinical practice and popular culture in recent years, giving rise to extensive research and commentary. Critical responses in the social sciences have criticised the cultural assumptions built in to the construct of 'hoarding disorder' and expressed fears that it may generate stigma outweighing its benefits; however, few of these studies have engaged directly with 'hoarders' themselves. This paper reports on in-depth, semi-structured interviews with 10 individuals living in England, who received assessment and intervention for hoarding from Social Services. Their narratives drew on the cultural repertoire of values and discourses around waste and worth, the mediation of sociality and relationships through material objects, physical constraints on keeping order and the role played by mental health. Analysing these perspectives anthropologically shows how dominant models of hoarding, such as the DSM-5 paradigm, potentially lend themselves to reductionist understandings that efface the meaning 'hoarding' may have and thereby deny agency to the person labelled as 'hoarder'. More culturally informed analysis, by contrast, affords insights into the complex landscape of value, waste, social critique, emotion, interpersonal relationships and practical difficulties that may underlie hoarding cases, and points the way to more person-centred practice and analysis.

  7. Do you see what I mean? Corticospinal excitability during observation of culture-specific gestures.

    Directory of Open Access Journals (Sweden)

    Istvan Molnar-Szakacs

    2007-07-01

    Full Text Available People all over the world use their hands to communicate expressively. Autonomous gestures, also known as emblems, are highly social in nature, and convey conventionalized meaning without accompanying speech. To study the neural bases of cross-cultural social communication, we used single pulse transcranial magnetic stimulation (TMS to measure corticospinal excitability (CSE during observation of culture-specific emblems. Foreign Nicaraguan and familiar American emblems as well as meaningless control gestures were performed by both a Euro-American and a Nicaraguan actor. Euro-American participants demonstrated higher CSE during observation of the American compared to the Nicaraguan actor. This motor resonance phenomenon may reflect ethnic and cultural ingroup familiarity effects. However, participants also demonstrated a nearly significant (p = 0.053 actor by emblem interaction whereby both Nicaraguan and American emblems performed by the American actor elicited similar CSE, whereas Nicaraguan emblems performed by the Nicaraguan actor yielded higher CSE than American emblems. The latter result cannot be interpreted simply as an effect of ethnic ingroup familiarity. Thus, a likely explanation of these findings is that motor resonance is modulated by interacting biological and cultural factors.

  8. THE INFLUENCE OF LANGUAGE COMPETENCE, WRITING COMPETENCE, AND CULTURAL COMPETENCE ON PRODUCING A SUCCESSFUL WRITING

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    Hermanto Hermanto

    2008-11-01

    Full Text Available Writing is a skill derived from a long way of learning and exercises. Different from other language skills, writing is considered the difficult language skill to acquire since it involves many aspects of linguistics, social, and writing knowledge and conventions. There are at least three important elements of writing useful to produce a good piece of composition, language competence, writing competence and cultural competence. This paper shows the influence of these three elements in order to produce good, readable, communicative, and successful writing

  9. (Independent meaning: Representations of addiction and recovery in selected poems produced in a rehabilitation centre

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    Antoinette Pretorius

    2017-08-01

    Full Text Available In this article we provide a close reading of selected poems written during creative writing workshops at a drug rehabilitation centre. We argue that these poems expose some of the uncertainties and complexities that characterise the representation of identity in experiences of addiction and recovery. We show that the speakers in these poems attempt to imagine and represent their experiences in language through a number of structuring binaries. These binaries include those between the speaker’s experiences of active addiction and recovery, and the speaker’s personal experience versus societal expectations and perceptions. Our reading of these poems is informed by the clinical context in which they were written, and our analysis reflects the bifurcation that governs this liminal space. Individual agency in these different spheres is approached in a very tentative way, and the speakers in these poems are shown to have trouble envisioning the future at the same time as their pasts appear unsettled. We argue finally that while current discourses and vocabularies surrounding addiction seem incomplete and inadequate for the expression of some complex experiences, poetry provides a platform that accommodates ambivalence and a multiplicity of meanings.

  10. Integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures.

    Science.gov (United States)

    Patel, Sanjay K S; Kumar, Prasun; Singh, Mamtesh; Lee, Jung-Kul; Kalia, Vipin C

    2015-01-01

    Biological production of hydrogen (H2) and polyhydroxybutyrate (PHB) from pea-shell slurry (PSS) was investigated using defined mixed culture (MMC4, composed of Enterobacter, Proteus, Bacillus spp.). Under batch culture, 19.0LH2/kg of PSS (total solid, TS, 2%w/v) was evolved. Using effluent from the H2 producing stage, Bacillus cereus EGU43 could produce 12.4% (w/w) PHB. Dilutions of PSS hydrolysate containing glucose (0.5%, w/v) resulted in 45-75LH2/kg TS fed and 19.1% (w/w) of PHB content. Under continuous culture, MMC4 immobilized on coconut coir (CC) lead to an H2 yield of 54L/kg TS fed and a PHB content of 64.7% (w/w). An improvement of 2- and 3.7-fold in H2 and PHB yields were achieved in comparison to control. This integrative approach using defined set of bacterial strains can prove effective in producing biomolecules from biowastes. Copyright © 2014. Published by Elsevier Ltd.

  11. USING THE MEANS OF THEATRE ART TO DEVELOP MODERN TEACHERS’ RHETORICAL CULTURE

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    Dmytro Budianskyi

    2017-04-01

    Full Text Available The article examines an important problem of modern education – the use of theatre arts to improve the teachers’ oratorical skills. In the pedagogical context the main categories of theatre pedagogy (co-creation, reincarnation, tempo, rhythm, mise-en-scène, etc. are discussed in details. Thus the congruence of teaching and dramatic art is found out. The essence of artistry is determined as an element of a teacher’s rhetorical culture. The contents, structural elements of Stanislavsky system and their use for developing the teacher’s rhetorical culture are analyzed. The author discusses in details the two main components of Stanislavsky system: an actor’s work itself and the actor’s work on the role. It is proved that both parts of this system with a few adjustments correspond to the method of forming the teacher-speaker’s artistic and rhetorical qualities. The development of teacher’s external techniques includes the preparation of the bodily apparatus for teaching. According to the author’s point of view, a teacher like an actor should have the mental technique to be able to call creative feeling at the right time. Special attention is paid to the various types of artistic training, which is an effective tool for developing the teacher’s rhetorical culture. The system of training includes exercises to develop speech culture, plastic expression, emotional memory, behavior naturalness, etc. The article focuses on the need to study and use the main principles of Stanislavsky system in the teachers’ practical activities. The most effective means of creating a creative mood is physical activity. The author emphasizes the necessity of developing modern-teachers’ artistic and rhetorical qualities by means of theatre pedagogy with the aim of increasing the efficiency of the educational process. The results of the study can be used in the process of training, professional activities and advanced training of education workers.

  12. O cinema como pedagogia cultural: significações por mulheres idosas Cinema as cultural pedagogy: meanings by elderly women

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    Wânia Ribeiro Fernandes

    2010-04-01

    Full Text Available Este artigo, fundamentado no campo dos estudos culturais - sobretudo em noções foucaultianas - e dos estudos feministas, analisa a contribuição do cinema na formação das identidades de gênero de mulheres idosas. Após breve discussão de aspectos relacionados à cultura, ao poder e à conformação das identidades de gênero, buscamos identificar, através das produções discursivas, significados construídos por um grupo de mulheres idosas sobre filmes a que assistiram em sua juventude, evidenciando os efeitos que os discursos dessa mídia exerceram na construção e na produção identitária dessas mulheres.Using the theoretical reference of the cultural studies- mainly some foucauldian and feminist studies notions- this paper focuses upon the influence of films on the construction of gender identities. After a brief discussion on aspects of culture, power and identities formation, the meanings established by a group of elderly women on films they have attended during their youth are analyzed. We bring into evidence the effects that thediscourses present in that media had upon those women's identitary construction and production.

  13. The role of acid incubation in rapid immobilization of hydrogen-producing culture in anaerobic upflow column reactors

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Zhen-Peng; Tay, Joo-Hwa [School of Civil and Environmental Engineering, Nanyang Technological University (Singapore); Institute of Environmental Science and Engineering, Nanyang Technological University (Singapore); Show, Kuan-Yeow [Faculty of Science, Engineering and Technology, University Tunku Abdul Rahman, 31900 Kampar, Perak (Malaysia); Liang, David Tee [Institute of Environmental Science and Engineering, Nanyang Technological University (Singapore); Lee, Duu-Jong [Department of Chemical Engineering, National Taiwan University, Taipei 10617 (China); Su, Ay [Department of Mechanical Engineering, Fuel Cell Center, Yuan-Ze University, Taoyuan 320 (China)

    2008-10-15

    An approach of acidification was examined on formation of hydrogen-producing granules and biofilms in upflow column-shaped reactors. The reactors were fed with synthetic glucose wastewater and operated at 37 C and pH 5.5. The acclimated anaerobic culture was inoculated in four reactors designated R1, R2, R3 and R4, with R3 and R4 filled with granular activated carbon as support medium. To unveil the roles of acidification, microbial culture in R2 and R3 was subject to an acid incubation for 24 h by shifting the culture pH from 5.5 to 2.0. The experimental results suggested that the acidification substantially accelerated microbial granulation, but not biofilm formation. Microbial activities were inhibited by the acid incubation for about 78 h, resulting in the retarded formation of biofilms of the acidified culture. Reducing culture pH resulted in improvement in cell surface physicochemical properties favoring microbial adhesion and immobilization. Zeta potential increased from -25.3 mV to 11.9 mV, hydrophobicity in terms of contact angle improved from 31 to 38 and production of extracellular polymers increased from 66 mg/g-VSS to 136 mg/g-VSS. As a result of the formation of granules and biofilms, high hydrogen production rates of 6.98 and 7.49 L/L h were achieved in granule-based and biofilm-based reactors, respectively. It is concluded that acid incubation is an efficient means to initiate the rapid formation of granules by regulating the surface characteristics of microbial culture. The use of support media as starting nuclei may result in rapid formation of biofilms without the acidification. (author)

  14. The role of acid incubation in rapid immobilization of hydrogen-producing culture in anaerobic upflow column reactors

    International Nuclear Information System (INIS)

    Zhang, Zhen-Peng; Tay, Joo-Hwa; Show, Kuan-Yeow; Liang, David Tee; Lee, Duu-Jong; Su, Ay

    2008-01-01

    An approach of acidification was examined on formation of hydrogen-producing granules and biofilms in upflow column-shaped reactors. The reactors were fed with synthetic glucose wastewater and operated at 37 C and pH 5.5. The acclimated anaerobic culture was inoculated in four reactors designated R1, R2, R3 and R4, with R3 and R4 filled with granular activated carbon as support medium. To unveil the roles of acidification, microbial culture in R2 and R3 was subject to an acid incubation for 24 h by shifting the culture pH from 5.5 to 2.0. The experimental results suggested that the acidification substantially accelerated microbial granulation, but not biofilm formation. Microbial activities were inhibited by the acid incubation for about 78 h, resulting in the retarded formation of biofilms of the acidified culture. Reducing culture pH resulted in improvement in cell surface physicochemical properties favoring microbial adhesion and immobilization. Zeta potential increased from -25.3 mV to 11.9 mV, hydrophobicity in terms of contact angle improved from 31 to 38 and production of extracellular polymers increased from 66 mg/g-VSS to 136 mg/g-VSS. As a result of the formation of granules and biofilms, high hydrogen production rates of 6.98 and 7.49 L/L h were achieved in granule-based and biofilm-based reactors, respectively. It is concluded that acid incubation is an efficient means to initiate the rapid formation of granules by regulating the surface characteristics of microbial culture. The use of support media as starting nuclei may result in rapid formation of biofilms without the acidification. (author)

  15. Parental grief and memento mori photography: narrative, meaning, culture, and context.

    Science.gov (United States)

    Blood, Cybele; Cacciatore, Joanne

    2014-01-01

    Postmortem photography is a widespread practice in perinatal bereavement care, yet few studies have explored how it affects bereaved parents, or how it might be received by parents of older children. This study is an examination of the meaning, utility, and social context of postmortem photography in a sample of 181 bereaved parents. Data were subjected to both quantitative and qualitative analysis. Photographs were positively regarded by most parents after perinatal death and several parents of older children. Other parents rejected postmortem photography for aesthetic, personal, or cultural reasons. Brief recommendations are offered for healthcare providers.

  16. Neuropharmacological and neuroprotective activities of some metabolites produced by cell suspension culture of Waltheria americana Linn.

    Science.gov (United States)

    Mundo, Jorge; Villeda-Hernández, Juana; Herrera-Ruiz, Maribel; Gutiérrez, María Del Carmen; Arellano-García, Jesús; León-Rivera, Ismael; Perea-Arango, Irene

    2017-10-01

    Waltheria americana is a plant used in Mexican traditional medicine to treat some nervous system disorders. The aims of the present study were to isolate and determine the neuropharmacological and neurprotective activities of metabolites produced by a cell suspension culture of Waltheria americana. Submerged cultivation of W. americana cells provided biomass. A methanol-soluble extract (WAsc) was obtained from biomass. WAsc was fractionated yielding the chromatographic fractions 4WAsc-H 2 O and WAsc-CH 2 Cl 2 . For the determination of anticonvulsant activity in vivo, seizures were induced in mice by pentylenetetrazol (PTZ). Neuropharmacological activities (release of gamma amino butyric acid (GABA) and neuroprotection) of chromatographic fractions were determined by in vitro histological analysis of brain sections of mice post mortem. Fraction 4WAsc-H 2 O (containing saccharides) did not produce neuronal damage, neurodegeneration, interstitial tissue edema, astrocytic activation, nor cell death. Pretreatment of animals with 4WAsc-H 2 O and WAsc-CH 2 Cl 2 from W. americana cell suspensions induced an increase in: GABA release, seizure latency, survival time, neuroprotection, and a decrease in the degree of severity of tonic/tonic-clonic convulsions, preventing PTZ-induced death of up to 100% of animals of study. Bioactive compounds produced in suspension cell culture of W. americana produce neuroprotective and neuropharmacological activities associated with the GABAergic neurotransmission system. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  17. Prevalence of extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated from blood cultures in Africa.

    Science.gov (United States)

    Sangare, S A; Maiga, A I; Guindo, I; Maiga, A; Camara, N; Savadogo, S; Diallo, S; Bougoudogo, F; Armand-Lefevre, L; Andremont, A; Maiga, I I

    2015-09-01

    Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae have been isolated from many regions of the world. Epidemiological studies are being conducted in Europe, North America, and Asia. No study has however been conducted in Africa to determine the prevalence and distribution of ESBLs on the continent. This literature review aimed at describing the prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures, as well as the ESBL genes involved at the international level. Our focus was mainly on Africa. We conducted a literature review on PubMed. Articles related to our study field and published between 1996 and 2014 were reviewed and entirely read for most of them, while we only focused on the abstracts of some other articles. Relevant articles to our study were then carefully reviewed and included in the review. The prevalence of ESBL-producing Enterobacteriaceae differs from one country to another. The results of our literature review however indicate that class A ESBLs prevail over the other types. We took into consideration articles focusing on various types of samples to assess the prevalence of ESBL-producing Enterobacteriaceae, but information on isolates from blood cultures is limited. The worldwide prevalence of ESBL-producing Enterobacteriaceae has increased over time. Evidence of ESBL-producing Enterobacteriaceae can be found in all regions of the world. Studies conducted in Africa mainly focused on the Northern and Eastern parts of the continent, while only rare studies were carried out in the rest of the continent. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  18. Formation of pedagogical system for individual selfdevelopment by means of physical culture and sport

    Directory of Open Access Journals (Sweden)

    Valery Panachev

    2017-11-01

    Full Text Available Problems of formation, development and introduction of the modern pedagogical selfdevelopment system in university educational process by means of physical culture and sport have been considered in this article. Such generated pedagogical system reflects practical implementation of social order on the modern educational paradigm aimed at creation of competitive, physically and morally strong individuals. This system promotes selfrealization of students’ individuality in formation of physical culture and sport competencies as well as competencies of selfdevelopment. Contemporary conditions of society’s development and analysis of the world social cultural and educational tendencies show that recently the object of scrupulous society’s attention and the subject of interdisciplinary research have become different aspects of person’s behavior in respect of his health in many highly developed countries. The slogan of such relation is: “Health is not everything but everything without health is nothing”. And this very principle specifies the problems of students’ selfdevelopment during intensive preparation for professional activity in the course of university training. These problems are aimed at maintenance of their health and improvement of physical preparation considering formation of professional motor competences for effective and qualitative mastering of future profession and career after graduation from the university.

  19. Ex Vivo Produced Oral Mucosa Equivalent by Using the Direct Explant Cell Culture Technique

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    Kamile Öztürk

    2012-09-01

    Full Text Available Objective: The aim of this study is the histological and immunohistochemical evaluation of ex vivo produced oral mucosal equivalents using keratinocytes cultured by direct explant technique.Material and Methods: Oral mucosa tissue samples were obtained from the keratinized gingival tissues of 14 healthy human subjects. Human oral mucosa keratinocytes from an oral mucosa biopsy specimen were dissociated by the explant technique. Once a sufficient population of keratinocytes was reached, they were seeded onto the type IV collagen coated “AlloDerm” and taken for histological and immunohistochemical examinations at 11 days postseeding of the keratinocytes on the cadaveric human dermal matrix.Results: Histopathologically and immunohistochemically, 12 out of 14 successful ex vivo produced oral mucosa equivalents (EVPOME that consisted of a stratified epidermis on a dermal matrix have been developed with keratinocytes cultured by the explant technique.Conclusion: The technical handling involved in the direct explant method at the beginning of the process has fewer steps than the enzymatic method and use of the direct explant technique protocol for culturing of human oral mucosa keratinocyte may be more adequate for EVPOME production.

  20. A portrait oil lamp from Pontes: Possible interpretations and meanings within early Byzantine visual culture

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    Petković Sofija

    2015-01-01

    Full Text Available The subject of this paper is a fragmented oil-lamp, discovered at Pontes (east Serbia dated to the 6th century, whose handle ending is shaped as a woman’s head. The question posed in this paper is whether the image of this woman could be identified as a portrait of some particular person or if it is just as a pictorial sign with some complicated symbolic meaning. The suggested identification alludes to the image of some of the empresses from the second half of the 6th century. [Projekat Ministarstva nauke Republike Srbije, br. 177007: Romanisation, urbanisation and transformation of urban centres of civil, military and residential character in Roman provinces in the territory of Serbia i br. 47018: Viminacium, Roman city and military camp - research of the material and non material culture of inhabitants by using the modern technologies of remote detection, geophysics, GIS, digitalization and3D visualization

  1. Experimental and mathematical model of the interactions in the mixed culture of links in the "producer-consumer" cycle

    Science.gov (United States)

    Pisman, T. I.; Galayda, Ya. V.

    The paper presents experimental and mathematical model of interactions between invertebrates the ciliates Paramecium caudatum and the rotifers Brachionus plicatilis and algae Chlorella vulgaris and Scenedesmus quadricauda in the producer -- consumer aquatic biotic cycle with spatially separated components The model describes the dynamics of the mixed culture of ciliates and rotifers in the consumer component feeding on the mixed algal culture of the producer component It has been found that metabolites of the algae Scenedesmus produce an adverse effect on the reproduction of the ciliates P caudatum Taking into account this effect the results of investigation of the mathematical model were in qualitative agreement with the experimental results In the producer -- consumer biotic cycle it was shown that coexistence is impossible in the mixed algal culture of the producer component and in the mixed culture of invertebrates of the consumer component The ciliates P caudatum are driven out by the rotifers Brachionus plicatilis

  2. Enzymatic activities produced by mixed Saccharomyces and non-Saccharomyces cultures: relationship with wine volatile composition.

    Science.gov (United States)

    Maturano, Yolanda Paola; Assof, Mariela; Fabani, María Paula; Nally, María Cristina; Jofré, Viviana; Rodríguez Assaf, Leticia Anahí; Toro, María Eugenia; Castellanos de Figueroa, Lucía Inés; Vazquez, Fabio

    2015-11-01

    During certain wine fermentation processes, yeasts, and mainly non-Saccharomyces strains, produce and secrete enzymes such as β-glucosidases, proteases, pectinases, xylanases and amylases. The effects of enzyme activity on the aromatic quality of wines during grape juice fermentation, using different co-inoculation strategies of non-Saccharomyces and Saccharomyces cerevisiae yeasts, were assessed in the current study. Three strains with appropriate enological performance and high enzymatic activities, BSc562 (S. cerevisiae), BDv566 (Debaryomyces vanrijiae) and BCs403 (Candida sake), were assayed in pure and mixed Saccharomyces/non-Saccharomyces cultures. β-Glucosidase, pectinase, protease, xylanase and amylase activities were quantified during fermentations. The aromatic profile of pure and mixed cultures was determined at the end of each fermentation. In mixed cultures, non-Saccharomyces species were detected until day 4-5 of the fermentation process, and highest populations were observed in MSD2 (10% S. cerevisiae/90% D. vanrijiae) and MSC1 (1% S. cerevisiae/99% C. sake). According to correlation and multivariate analysis, MSD2 presented the highest concentrations of terpenes and higher alcohols which were associated with pectinase, amylase and xylanase activities. On the other hand, MSC1 high levels of β-glucosidase, proteolytic and xylanolytic activities were correlated to esters and fatty acids. Our study contributes to a better understanding of the effect of enzymatic activities by yeasts on compound transformations that occur during wine fermentation.

  3. Chemical evaluation of strawberry plants produced by tissue culturing of gamma irradiated seedlings

    International Nuclear Information System (INIS)

    Maraei, R.W.

    2007-01-01

    studies were conducted to evaluate the influence of gamma irradiation as a supplementary factor precedes tissue culture application on strawberry seedlings (c.v.Rosa Linda). the strawberry seedling were irradiated using 8 doses of co 60 gamma rays 50.75.100.125 ,150,250, 350 and 500 gray. tissue culture technique was applied on irradiated and unirradiated strawberry seedling. different characteristics of plantlets, plant and fruit of strawberry produced from the double treatment (irradiation followed by tissue culture) were studied as well as the early, total and exportable fruit yields. data indicated that, low radiation doses 50,75 and 100 gray increased all morphological and chemical characteristics of the plantlets, plant and fruit of strawberry, whereas radiation doses higher than 100 gray decreased them significantly. moreover 350 and gray were lethal doses. radiation dose 50 gray increased the survival percentage and the length of plantlets by 1.5% and 50% respectively more than the unirradiated treatment in all multiplication stages

  4. Lipolytic Changes in Fermented Sausages Produced with Turkey Meat: Effects of Starter Culture and Heat Treatment.

    Science.gov (United States)

    Karsloğlu, Betül; Çiçek, Ümran Ensoy; Kolsarici, Nuray; Candoğan, Kezban

    2014-01-01

    In this study, the effects of two different commercial starter culture mixes and processing methodologies (traditional and heat process) on the lipolytic changes of fermented sausages manufactured with turkey meat were evaluated during processing stages and storage. Free fatty acid (FFA) value increased with fermentation and during storage over 120 d in all fermented sausage groups produced with both processing methodologies (p<0.05). After drying stage, free fatty acid values of traditional style and heat processed fermented sausages were between 10.54-13.01% and 6.56-8.49%, respectively. Thiobarbituric acid (TBA) values of traditionally processed fermented sausages were between 0.220-0.450 mg·kg(-1), and TBA values of heat processed fermented sausages were in a range of 0.405-0.795 mg·kg(-1). Oleic and linoleic acids were predominant fatty acids in all fermented sausages. It was seen that fermented sausage groups produced with starter culture had lower TBA and FFA values in comparison with the control groups, and heat application inhibited the lipase enzyme activity and had an improving effect on lipid oxidation. As a result of these effects, heat processed fermented sausages had lower FFA and higher TBA values than the traditionally processed groups.

  5. Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali.

    Science.gov (United States)

    Sangare, Samba Adama; Maiga, Almoustapha Issiaka; Guindo, Ibrehima; Maiga, Aminata; Camara, Namory; Dicko, Oumar Agaly; Diallo, Souleymane; Bougoudogo, Flabou; Armand-Lefevre, Laurence; Andremont, Antoine; Maiga, Ibrahim Izetiegouma

    2016-10-31

    The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients' blood cultures in two university teaching hospitals of Bamako, Mali. During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated. The microbial identifications were initially performed with an API 20E gallery and VITEK2 locally in Mali, and then confirmation in France was performed with a mass spectrometry MALDI-TOF in the bacteriology laboratory of the university teaching hospital of Bichat. Antibiotic susceptibility profiles were determined by the diffusion method as recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The isolated species were K. pneumoniae (14/40; 35.0%), E. coli (11/40; 27.5%), and E. cloacae (9/40; 22.5%). Of the strains isolated, 21/34 (61.8%) had an ESBL phenotype, including 10/14 (71.4%) K. pneumoniae, 8/11 (72.7%) E. coli, and 3/9 (33.3%) E. cloacae. Resistances associated with ESBL strains of K. pneumoniae, E. coli, and E. cloacae were as follows: gentamicin (10/10, 100%; 6/8, 75%; 2/3, 67%, respectively), amikacin (2/10, 20%; 0/8, 0%; 0/3, 0%, respectively), ofloxacin (8/10, 80%; 7/8, 87%; 3/3, 100%, respectively), and cotrimoxazole (10/10, 100%; 6/8, 75%; 3/3, 100%, respectively). Almost two-thirds (61.8%) of Enterobacteriaceae isolated from our blood cultures were ESBL producers. Only susceptibilities to carbapenems and to amikacin were fully conserved within the strains.

  6. Significados culturais da asma infantil Cultural meanings of the infantile asthma

    Directory of Open Access Journals (Sweden)

    Mirna Albuquerque Frota

    2008-06-01

    Full Text Available OBJETIVO: Analisar os significados culturais da asma infantil com origem na mãe-cuidadora. PROCEDIMENTOS METODOLÓGICOS: Pesquisa qualitativa realizada em 2004, no município de Fortaleza, CE. Foi utilizada a observação participante com abordagem etnográfica e entrevistas com sete mães acompanhantes de seus filhos em unidade de emergência hospitalar. Os significados das falas das mães foram identificados utilizando-se a técnica de análise temática. ANÁLISE DOS RESULTADOS: Foram identificadas duas categorias de discussão; na primeira, "desinformação sobre a doença", foi possível perceber que as mães não são informadas sobre a doença do filho. Na segunda categoria, "cuidado cultural", as mães referem informações sobre os cuidados e utilizam recursos do saber popular para prevenir a asma dos filhos, como o cuidado ambiental e a utilização de remédios caseiros à base de plantas medicinais. CONCLUSÕES: As características de desinformação e desconhecimento materno em relação à asma do filho mostram a necessidade de haver um trabalho educativo intenso, dialógico e problematizador em estreita colaboração com o tratamento, visando à melhoria do prognóstico da doença.OBJECTIVE: To analyze the cultural meanings of infantile asthma from the perspective of the mother/carer of the child. METHODOLOGICAL PROCEDURES: Qualitative research conducted in 2004, in the city of Fortaleza (Northeastern Brazil. An ethnographic approach was utilized, consisting of participant observation and interviews with seven mothers, accompanying their children in a hospital emergency ward. Thematic analysis was the technique employed when identifying the meanings of mothers' discourse. ANALYSIS OF RESULTS: Two discussion categories were identified: "disinformation on illness" in which it was possible to perceive that the mothers were not informed with respect to their children's illness; and "cultural care" in which they relate information on

  7. Cultural Meaning of Perceived Control: A Meta-Analysis of Locus of Control and Psychological Symptoms across 18 Cultural Regions

    Science.gov (United States)

    Cheng, Cecilia; Cheung, Shu-fai; Chio, Jasmine Hin-man; Chan, Man-pui Sally

    2013-01-01

    Integrating more than 40 years of studies on locus of control (LOC), this meta-analysis investigated whether (a) the magnitude of the relationship between LOC and psychological symptoms differed among cultures with distinct individualist orientations and (b) depression and anxiety symptoms yielded different patterns of cultural findings with LOC.…

  8. Nearly Finished Genomes Produced Using Gel Microdroplet Culturing (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    Energy Technology Data Exchange (ETDEWEB)

    Fitzsimmons, Michael

    2012-06-01

    Michael Fitzsimmons from Los Alamos National Laboratory gives a talk titled "Nearly Finished Genomes Produced Using Gel Microdroplet Culturing" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  9. Experiments with a First Prototype of a Spatial Model of Cultural Meaning through Natural-Language Human-Robot Interaction

    Directory of Open Access Journals (Sweden)

    Oliver Schürer

    2018-01-01

    Full Text Available When using assistive systems, the consideration of individual and cultural meaning is crucial for the utility and acceptance of technology. Orientation, communication and interaction are rooted in perception and therefore always happen in material space. We understand that a major problem lies in the difference between human and technical perception of space. Cultural policies are based on meanings including their spatial situation and their rich relationships. Therefore, we have developed an approach where the different perception systems share a hybrid spatial model that is generated by artificial intelligence—a joint effort by humans and assistive systems. The aim of our project is to create a spatial model of cultural meaning based on interaction between humans and robots. We define the role of humanoid robots as becoming our companions. This calls for technical systems to include still inconceivable human and cultural agendas for the perception of space. In two experiments, we tested a first prototype of the communication module that allows a humanoid to learn cultural meanings through a machine learning system. Interaction is achieved by non-verbal and natural-language communication between humanoids and test persons. This helps us to better understand how a spatial model of cultural meaning can be developed.

  10. Biofilm formation by designed co-cultures of Caldicellulosiruptor species as a means to improve hydrogen productivity.

    Science.gov (United States)

    Pawar, Sudhanshu S; Vongkumpeang, Thitiwut; Grey, Carl; van Niel, Ed Wj

    2015-01-01

    Caldicellulosiruptor species have gained a reputation as being among the best microorganisms to produce hydrogen (H2) due to possession of a combination of appropriate features. However, due to their low volumetric H2 productivities (Q H2), Caldicellulosiruptor species cannot be considered for any viable biohydrogen production process yet. In this study, we evaluate biofilm forming potential of pure and co-cultures of Caldicellulosiruptor saccharolyticus and Caldicellulosiruptor owensensis in continuously stirred tank reactors (CSTR) and up-flow anaerobic (UA) reactors. We also evaluate biofilms as a means to retain biomass in the reactor and its influence on Q H2. Moreover, we explore the factors influencing the formation of biofilm. Co-cultures of C. saccharolyticus and C. owensensis form substantially more biofilm than formed by C. owensensis alone. Biofilms improved substrate conversion in both of the reactor systems, but improved the Q H2 only in the UA reactor. When grown in the presence of each other's culture supernatant, both C. saccharolyticus and C. owensensis were positively influenced on their individual growth and H2 production. Unlike the CSTR, UA reactors allowed retention of C. saccharolyticus and C. owensensis when subjected to very high substrate loading rates. In the UA reactor, maximum Q H2 (approximately 20 mmol · L(-1)  · h(-1)) was obtained only with granular sludge as the carrier material. In the CSTR, stirring negatively affected biofilm formation. Whereas, a clear correlation was observed between elevated (>40 μM) intracellular levels of the secondary messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) and biofilm formation. In co-cultures C. saccharolyticus fortified the trade of biofilm formation by C. owensensis, which was mediated by elevated levels of c-di-GMP in C. owensensis. These biofilms were effective in retaining biomass of both species in the reactor and improving Q H2 in a UA reactor using

  11. The philosophical and educational potential ecopsychology as means of forming ecological culture of personality

    Directory of Open Access Journals (Sweden)

    O. V. Kisyel

    2014-09-01

    Full Text Available The current ecological crisis is largely caused by the dominance of anthropocentric environmental awareness. To overcome it and move humanity to a model of sustainable social and environmental development required the establishment of environmental consciousness ecocentric type, which is the aim of environmental education, namely, the formation of personality type ecocentric environmental awareness. The purpose of the article is to study the philosophical and educational potential environmental psychology as a means of transformation of personal values and attitudes toward nature. Analysis of the concepts of subjective attitude to nature, subjective perception of the natural world, environmental knowledge, empaurment-method pedagogy. Education for Sustainable Development aims to develop such knowledge, skills and values that will enable people to make individual and collective decisions of local and global nature to improve the quality of life without endangering future generations. The basic methodological principle of environmental psyhopedahohiky is in strict accordance with the environmental education teaching psychological process of environmental awareness. Interaction with nature is a great psychological and pedagogical potential that should be used in the environmental education that will allow him to become a factor in the overall development and personality development. So effectively targeted formation ekospozhyvchoyi culture of all populations requires training of qualified specialists in the field of eco-educational activities and environmental education and its philosophical and educational foundation.

  12. ["Birth weakness": cultural meanings of maternal impressions for infant health in Northeast Brazil].

    Science.gov (United States)

    Calvasina, Paola Gondim; Nations, Marilyn K; Jorge, Maria Salete Bessa; Sampaio, Helena Alves de Carvalho

    2007-02-01

    Pregnancy encompasses socio-cultural, historical, and affective dimensions that process various meanings in women's bodies. Each society constructs popular concepts, practices, and explanatory models that differ from the biomedical model and aim to protect the mother and fetus and foster a healthy pregnancy. This qualitative study, based on interpretative anthropology, unveils the experiences of 27 poor women and their repercussions on the malnutrition of their infants, treated at a Childhood Malnutrition Treatment Center in Fortaleza, Northeast Brazil. From January to June 2004, ethnographic and narrative interviews were conducted on so-called "birth weakness", in addition to participant observation of outpatient, nursing, and home childcare. The mothers believed that their own physical and emotional suffering and precarious nutritional status were "imprinted" on the fetus, resulting in the child's malnutrition. While the ethno-etiology of "weakness" points to factors outside the body that involuntarily affect the pregnant mother, the medical view tended to incriminate the mother herself. It is necessary to understand the mothers' narratives, sympathize with their suffering, and spawn a closer approach between the popular and biomedical concepts.

  13. Technical means and system guarantee of museumprotection and inheritance for intangible cultural heritage

    Institute of Scientific and Technical Information of China (English)

    TANG Zhiqiang

    2015-01-01

    Intangible cultural heritage has a rich connotation of unique thinking way, ethical imaginationand cultural awareness, which is an important sign of national spiritual culture. Possessing the specialvalues of professionalism, permanency, security and openness, with the advantages museums play acentral role in inheriting and rescuing our intangible cultural heritage. Museums have a lot of technicalmeans for museums to protect and inherit intangible cultural heritage, such as scientific research,preservation of material carrier, museum publications, displays on intangible cultural heritage, estab-lishing thematic museums. In intangible cultural heritage, agricultural heritage is the most importantpart. The core content of which is the ideas and methods of harmony between mankind and environ-ment. There is a variety of practices on the protection by China National Agricultural Museum.

  14. Culture.

    Science.gov (United States)

    Smith, Timothy B; Rodríguez, Melanie Domenech; Bernal, Guillermo

    2011-02-01

    This article summarizes the definitions, means, and research of adapting psychotherapy to clients' cultural backgrounds. We begin by reviewing the prevailing definitions of cultural adaptation and providing a clinical example. We present an original meta-analysis of 65 experimental and quasi-experimental studies involving 8,620 participants. The omnibus effect size of d = .46 indicates that treatments specifically adapted for clients of color were moderately more effective with that clientele than traditional treatments. The most effective treatments tended to be those with greater numbers of cultural adaptations. Mental health services targeted to a specific cultural group were several times more effective than those provided to clients from a variety of cultural backgrounds. We recommend a series of research-supported therapeutic practices that account for clients' culture, with culture-specific treatments being more effective than generally culture-sensitive treatments. © 2010 Wiley Periodicals, Inc.

  15. A plant culture system for producing food and recycling materials with sweetpotato in space

    Science.gov (United States)

    Kitaya, Yoshiaki; Yano, Sachiko; Hirai, Hiroaki

    2016-07-01

    The long term human life support in space is greatly dependent on the amounts of food, atmospheric O2 and clean water produced by plants. Therefore, the bio-regenerative life support system such as space farming with scheduling of crop production, obtaining high yields with a rapid turnover rate, converting atmospheric CO2 to O2 and purifying water should be established with employing suitable plant species and varieties and precisely controlling environmental variables around plants grown at a high density in a limited space. We are developing a sweetpotato culture system for producing tuberous roots as a high-calorie food and fresh edible leaves and stems as a nutritive functional vegetable food in space. In this study, we investigated the ability of food production, CO2 to O2 conversion through photosynthesis, and clean water production through transpiration in the sweetpotato production system. The biomass of edible parts in the whole plant was almost 100%. The proportion of the top (leaves and stems) and tuberous roots was strongly affected by environmental variables even when the total biomass production was mostly the same. The production of biomass and clean water was controllable especially by light, atmospheric CO2 and moisture and gas regimes in the root zone. It was confirmed that sweetpotato can be utilized for the vegetable crop as well as the root crop allowing a little waste and is a promising functional crop for supporting long-duration human activity in space.

  16. Study of the defect structures produced by heavy ions in dielectrics by means of small angle scattering

    International Nuclear Information System (INIS)

    Albrecht, D.J.

    1983-11-01

    The aim of the present thesis was to study the defects in dielectrics produced by fast ions. For this the small angle scattering was proved as suitable method. By the production by means of the ion beam of an accelerator the defects possess a pronounced preferential direction. In scattering experiments this system of scattering centers is distinguished by its unusually strong dependence of the sample orientation according to the primary beam. This property was studied, described, and illustrated by examples, and it could by shown that from this additional informations about the scattering defects can be derived. For the first time nuclear tracks were detected by means of small angle neutron scattering. It could be shown that here the same results are obtained as by small angle X-ray scattering. The measured intensity distributions could be assigned to a mathematical model description which gives form, width, and density of the tracks in the position space. On the base of this cylinder model computer codes were established which simulate the scattering experiment regarding the main influences and calculate the expected theoretical intensity distribution. The agreement between calculated and measured scattering distributions proves the validity of the model. The parameters determined by this model, maximal change of density in the track, defect length, radial dilatation, distance of the narrowings and there depth were determined. For the main quantities, radial dilatation and maximal change of densities a simple description of the energy dependence could be given via the energy loss. (orig./HSI) [de

  17. Culturing the Unculturable: Human Coronavirus HKU1 Infects, Replicates, and Produces Progeny Virions in Human Ciliated Airway Epithelial Cell Cultures

    NARCIS (Netherlands)

    Pyrc, Krzysztof; Sims, Amy C.; Dijkman, Ronald; Jebbink, Maarten; Long, Casey; Deming, Damon; Donaldson, Eric; Vabret, Astrid; Baric, Ralph; van der Hoek, Lia; Pickles, Raymond

    2010-01-01

    Culturing newly identified human lung pathogens from clinical sample isolates can represent a daunting task, with problems ranging from low levels of pathogens to the presence of growth suppressive factors in the specimens, compounded by the lack of a suitable tissue culture system. However, it is

  18. Experimental and mathematical model of the interactions in the mixed culture of links in the “producer-consumer” cycle

    Science.gov (United States)

    Pisman, T. I.

    2009-07-01

    The paper presents a experimental and mathematical model of interactions between invertebrates (the ciliates Paramecium caudatum and the rotifers Brachionus plicatilis) in the "producer-consumer" aquatic biotic cycle with spatially separated components. The model describes the dynamics of the mixed culture of ciliates and rotifers in the "consumer" component, feeding on the mixed algal culture of the "producer" component. It has been found that metabolites of the algae Scenedesmus produce an adverse effect on the reproduction of the ciliates P. caudatum. Taking into account this effect, the results of investigation of the mathematical model were in qualitative agreement with the experimental results. In the "producer-consumer" biotic cycle it was shown that coexistence is impossible in the mixed culture of invertebrates of the "consumer" component. The ciliates P. caudatum are driven out by the rotifers B. plicatilis.

  19. Rheology of spreadable goat cheese made with autochthonous lactic cultures differing in their ability to produce exopolysaccharides

    Directory of Open Access Journals (Sweden)

    Frau Silvia Florencia

    2013-06-01

    Full Text Available The aim of this study was to compare the rheology of spreadable cheeses elaborated with autochthonous lactic starter cultures without the addition of exopolysaccharide-producing strain in the same starter with exopolysaccharide-producing strain. From a rheological standpoint, both samples were characterized as weak viscoelastic gels and pseudoplastic products. It was concluded that cheese made with exopolysaccharide-producing strain showed smaller G', G", and η* values over the range of frequencies studied and smaller critic stress values than the cheese without exopolysaccharide-producing strain. The results obtained indicate that cheeses without exopolysaccharide-producing strain need to be added with any texture enhancer product.

  20. Growth of nutrient-replete Microcystis PCC 7806 cultures is inhibited by an extracellular signal produced by chlorotic cultures.

    Science.gov (United States)

    Dagnino, Denise; de Abreu Meireles, Diogo; de Aquino Almeida, João Carlos

    2006-01-01

    The frequency of cyanobacterial blooms has been increasing all over the world. These blooms are often toxic and have become a serious health problem. The aim of this work was to search for population density control mechanisms that could inhibit the proliferation of the toxic bloom-forming genus Microcystis. Microcystis PCC 7806 cultured for long periods in liquid ASM-1 medium loses its characteristic green colour. When a medium of chlorotic cultures is added to a nutrient-replete culture, cell density increase is drastically reduced when compared with controls. Inhibition of cell proliferation occurs in Microcystis cultures from any growth stage and was not strain-specific, but other genera tested showed no response. Investigations on the mechanism of growth inhibition showed that cultures treated with the conditioned medium acquired a pale colour, with pigment concentration similar to that found in chlorotic cultures. Ultrastructural examination showed that the conditioned medium induced thylakoid membrane disorganization, typical of chlorotic cells, in nutrient-replete cultures. An active extract was obtained and investigations showed that activity was retained after heating and after addition of an apolar solvent. This indicates that activity of the conditioned medium from chlorotic cells results from non-protein, apolar compound(s).

  1. On the Meaning of Cross-Cultural Differences in Simple Cognitive Measures

    Science.gov (United States)

    van de Vijver, Fons J. R.

    2008-01-01

    A set of 5 reaction time tests of increasing cognitive complexity were administered to 35 secondary school pupils in Zimbabwe and The Netherlands at 4 consecutive school days in order to explore the existence and nature of cross-cultural differences on reaction time tests measuring basic cognitive operations. No cross-cultural differences were…

  2. Developing the Pedagogical Culture of Parents by Means of Social Partnership with a Supplementary Education Institution

    Science.gov (United States)

    Zakirova, Venera G.; Nikitina, Ekaterina L.

    2016-01-01

    The urgency of the research is due to the current requirements to develop the pedagogical culture of parents, which can help them to be successful parents, to effectively interact with their children avoiding family upbringing mistakes. In this respect, the article aims to identify the conditions for development of the pedagogical culture of…

  3. The Same but Different: Making Meaning from Modified Texts with Cross-Cultural Themes

    Science.gov (United States)

    Leung, Cynthia B.; Bennett, Susan V.; Gunn, AnnMarie Alberton

    2017-01-01

    Reader response theory provides the framework for the present study that explored literary elements and cultural responses of fifth-grade students to two modified versions of a cross-cultural text, "Homesick: My Own Story" by Jean Fritz. One group of students read the first chapter of the book and another group read a modified basal…

  4. Reading Difference: Picture Book Retellings as Contexts for Exploring Personal Meanings of Race and Culture

    Science.gov (United States)

    Lysaker, Judith; Sedberry, Tiffany

    2015-01-01

    In racially and culturally homogeneous school settings, opportunities for children to interact with those who are unlike themselves are not always available. Picture book retellings provide contexts within which students are exposed to racial and cultural differences by allowing them to engage in vicarious events with people they might not…

  5. Forming Health Culture of Bachelors of Education by Means of an Academic Course

    Science.gov (United States)

    Asafova, Elena V.; Sazanova, Maria L.

    2016-01-01

    In Russia the system of spreading health-culture among the young generation, the students, has not been formed yet, which makes the paper topical and up-to-date. The young generation is characterized by a low level of education and professional training efficiency in healthy life-style and health culture. It has caused depreciation of the concepts…

  6. Nigerian Students' Perceptions and Cultural Meaning Construction Regarding Academic Integrity in the Online International Classroom

    Science.gov (United States)

    Szilagyi, Annamaria

    2014-01-01

    By presenting perceptions of Nigerian students enrolled in the online international postgraduate programmes of the University of Liverpool regarding academic integrity, this paper aims to explore Western ideas, such as originality and plagiarism that are extraneous in the students' local cultures. Different historical and cultural circumstances…

  7. When does meaning making predict subjective well-being? Examining young and older adults in two cultures.

    Science.gov (United States)

    Alea, Nicole; Bluck, Susan

    2013-01-01

    Two studies in different cultures (Study 1: USA, N=174, Study 2: Trinidad, N=167) examined whether meaning making, (i.e., both searching for meaning, and directing behaviour) is positively related to subjective well-being (SWB) by age (younger, older adults). In both studies, participants self-reported engagement in meaning making, and SWB (e.g., affect, future time perspective, psychological well-being). In Study 1, young Americans (compared to older) more frequently used their past to direct behaviour but doing so was unrelated to SWB. In older Americans, both types of meaning making were positively associated with SWB. In Study 2, Trinidadian younger adults were again more likely than older adults to engage in meaning making. Unlike in the American sample, however, directing behaviour was positively related to SWB for both young and older adults. The studies demonstrate that whether meaning making shows benefits for SWB may depend on type of meaning, age and culture. Note that although meaning making was sometimes unrelated to SWB, no detrimental relations to meaning making were found. The discussion focuses on the role of moderators in understanding when meaning making should lead to benefits versus costs to SWB.

  8. Silence, nostalgia, violence, poverty … : what does 'culture' mean for South African sexuality educators?

    Science.gov (United States)

    Depalma, Renée; Francis, Dennis

    2014-01-01

    In in-depth interviews with 25 Life Orientation teachers in South Africa, we found that teachers spontaneously drew upon notions of culture to explain and justify people's sexual beliefs and behaviours and their own role as educators. Drawing upon a Bakhtinian understanding of discourse, we apply critical semantic analysis to explore how culture is deployed as a discursive strategy. Teachers draw upon particular understandings of culture available to them in their social contexts. Furthermore, the substitution of the word 'culture' for a series of other phenomena (silence, violence and poverty) affords these phenomena a certain authority that they would otherwise not wield. We argue, first, that systems teacher education and training needs to (re)define culture as dynamic, interactive and responding to, but not determined by, socio-historical realities. Beyond this, teachers need to learn how to critically engage with cultural practices and perceptions and to be provided with some basic tools to do so, including more sophisticated understandings of cultural and training in dialogic methodologies. Teaching sexuality education in multicultural societies such as South Africa will require meaningful engagement in intercultural dialogues that may need to include voices that have traditionally been excluded from school spaces.

  9. National character does not reflect mean personality trait levels in 49 cultures

    DEFF Research Database (Denmark)

    Terracciano, A; Abdel-Khalek, A M; Adám, N

    2005-01-01

    ratings of 3989 people from 49 cultures and compared them with the average personality scores of culture members assessed by observer ratings and self-reports. National character ratings were reliable but did not converge with assessed traits. Perceptions of national character thus appear to be unfounded......Most people hold beliefs about personality characteristics typical of members of their own and others' cultures. These perceptions of national character may be generalizations from personal experience, stereotypes with a "kernel of truth," or inaccurate stereotypes. We obtained national character...

  10. Prospective study on cranioplasty with individual carbon fiber reinforced polymer (CFRP) implants produced by means of stereolithography.

    Science.gov (United States)

    Wurm, Gabriele; Tomancok, Berndt; Holl, Kurt; Trenkler, Johannes

    2004-12-01

    The aim of this study was to evaluate the value of carbon fiber reinforced polymer (CFRP) cranial implants produced by means of 3-dimensional (3D) stereolithography (SL) and template modeling for reconstructions of complex or extensive cranial defects. A series of 41 cranioplasties with individual CFRP implants was performed in 37 patients between April 1996 and November 2002. Only patients with complex and/or large cranial defects were included, most of them having extended scarring or dural calcification and poor quality of the overlying soft-tissue cover after infection or multiple preceding operations. Involvement of frontal sinus, a known risk factor for complications after cranioplasty, was the case in 21 patients (51.2%). A computer-based 3D model of the skull with the bony defect was generated by means of stereolithography after acquisition, evaluation and transfer of the patient's helical computed tomography (CT) data. A wax template of the defect that was used to design the individual prosthesis-shape was invested in dental stone. Then, the cranial implant was fabricated out of CFRP by loosen mold. Reconstruction of defects measuring up to 17 x 9 cm was performed. The intra-operative fit of the implants was excellent in 36 (87.8%), good in 1 (2.4%), and fair in 4 (9.8%) of the cases. Problems of implant fit occurred because of extended scarring and poor quality of soft-tissue cover. Adverse reactions were observed in 5 patients (1 subdural, 1 subcutaneous hematoma, 2 infections, 1 allergic reaction). Excellent contours and a solid stable reconstruction have been maintained in 30 out of 35 remaining plates (mean follow-up 3.6 years). No adverse effects concerning postoperative imaging, the accuracy of electroencephalograms and radiation therapy have been observed. The authors believe that this relatively new technique represents an advance in the management of complex and large cranial defects, but seems less suitable for simple defects because of cost

  11. Design, Fabrication, and Operation of Innovative Microalgae Culture Experiments for the Purpose of Producing Fuels: Final Report, Phase I

    Energy Technology Data Exchange (ETDEWEB)

    1985-01-01

    A conceptual design was developed for a 1000-acre (water surface) algae culture facility for the production of fuels. The system is modeled after the shallow raceway system with mixing foils that is now being operated at the University of Hawaii. A computer economic model was created to calculate the discounted breakeven price of algae or fuels produced by the culture facility. A sensitivity analysis was done to estimate the impact of changes in important biological, engineering, and financial parameters on product price.

  12. Desacralising Shakespeare’s “word” by means of cultural translation/transposition

    Directory of Open Access Journals (Sweden)

    José Roberto O’Shea

    2016-09-01

    Full Text Available http://dx.doi.org/10.5007/2175-7968.2016v36n3p124 This essay addresses ways in which cultural translation/transposition can ultimately bring about a positive “desacralisation” of Shakespeare’s Word. The discussion starts from the notion of Shakespeare’s Word as “sacred” and of sacred writings as highly sensitive language, and proceeds to overview the importance of the notions of denotation, connotation, and context in translation. Then, the essay offers working definitions of cultural translation or cultural transposition, and of non-literal translation. Finally, the essay highlights the author’s main aims in translating Shakespeare’s theatre and offers a few examples of cultural translation/transposition in his own rendering of Shakespeare’s drama into Brazilian Portuguese.

  13. Desacralising Shakespeare’s “word” by means of cultural translation/transposition

    Directory of Open Access Journals (Sweden)

    José Roberto O’Shea

    2016-12-01

    Full Text Available This essay addresses ways in which cultural translation/transposition can ultimately bring about a positive “desacralisation” of Shakespeare’s Word. The discussion starts from the notion of Shakespeare’s Word as “sacred” and of sacred writings as highly sensitive language, and proceeds to overview the importance of the notions of denotation, connotation, and context in translation. Then, the essay offers working definitions of cultural translation or cultural transposition, and of non-literal translation. Finally, the essay highlights the author’s main aims in translating Shakespeare’s theatre and offers a few examples of cultural translation/transposition in his own rendering of Shakespeare’s drama into Brazilian Portuguese.

  14. Comparison of Sensory Properties, Shelf-Life and Microbiological Safety of Industrial Sausages Produced with Autochthonous and Commercial Starter Cultures

    Directory of Open Access Journals (Sweden)

    Jadranka Frece

    2014-01-01

    Full Text Available The aim of this research is to use isolated and characterized autochthonous functional starter cultures from traditional Croatian dry sausages and to evaluate their capacity for industrial production of five sausages (Čajna sausage, Zimska sausage, Bečka sausage, Srijemska sausage and Slavonski kulen. These defined autochthonous functional starter cultures (combination of Lactobacillus and Staphylococcus strains were used to produce five different industrial sausages which were compared by a panel. The viability of introduced autochthonous Lactobacillus and Staphylococcus strains and their effect on the final product characteristics, namely microbiological, physicochemical and sensory properties were monitored. The obtained results indicate that autochthonous starter cultures survived industrial production of sausages and can be used for production of sausages under controlled conditions. Autochthonous starter cultures obtained better results in the organoleptic evaluation, microbial safety and prolonged shelf-life in comparison with commercial starter cultures.

  15. Stochastic modelling of Listeria monocytogenes single cell growth in cottage cheese with mesophilic lactic acid bacteria from aroma producing cultures

    DEFF Research Database (Denmark)

    Østergaard, Nina Bjerre; Christiansen, Lasse Engbo; Dalgaard, Paw

    2015-01-01

    . 2014. Modelling the effect of lactic acid bacteria from starter- and aroma culture on growth of Listeria monocytogenes in cottage cheese. International Journal of Food Microbiology. 188, 15-25]. Growth of L. monocytogenes single cells, using lag time distributions corresponding to three different......A stochastic model was developed for simultaneous growth of low numbers of Listeria monocytogenes and populations of lactic acid bacteria from the aroma producing cultures applied in cottage cheese. During more than two years, different batches of cottage cheese with aroma culture were analysed...

  16. An Investigation into the Trueness and Precision of Copy Denture Templates Produced by Rapid Prototyping and Conventional Means.

    Science.gov (United States)

    Davda, K; Osnes, C; Dillon, S; Wu, J; Hyde, P; Keeling, A

    2017-12-01

    To assess the trueness and precision of copy denture templates produced using traditional methods and 3D printing. Six copies of a denture were made using: 1. Conventional technique with silicone putty in an impression tray (CT). 2. Conventional technique with no impression tray (CNT). 3. 3D scanning and printing (3D). Scan trueness and precision was investigated by scanning a denture six times and comparing five scans to the sixth. Then the scans of the six CT, CNT and 3D dentures were compared by aligning, in turn, the copies of each denture to the scanned original. Outcome measures were the mean surface-to-surface distance, standard deviation of that distance and the maximum distance. Student's unpaired t-tests with Bonferroni correction were used to analyse the results. The repeated scans of the original denture showed a scan trueness of 0.013mm (SD 0.002) and precision of 0.013mm (SD 0.002). Trueness: CT templates, 0.168mm (0.047), CNT templates 0.195mm (0.034) and 3D 0.103mm (0.021). Precision: CT templates 0.158mm (0.037), CNT 0.233mm (0.073), 3D 0.090mm (0.017). For each outcome measure the 3D templates demonstrated an improvement which was statistically significant (p⟨0.05). 3D printed copy denture templates reproduced the original with greater trueness and precision than conventional techniques. Copyright© 2017 Dennis Barber Ltd.

  17. Folk national culture as a means of forming norms of communication in childhood

    Directory of Open Access Journals (Sweden)

    Chernushevich V. A.

    2016-06-01

    Full Text Available The best carriers of playing culture are children, who possess and enjoy it. Destroyed social kids’ structures, territorial kids’ associations (family, yard, village, street communities of children interrupted generally the process of culture transmission, reproduction and passing of communication tradition. And there is a need in social-state “revivification” (recovering folk games list and its’ players, enough for folk games reproduction process. Folk game includes particular properties of relations on the levels of physical and emotional, vocal interaction, imagery-symbolic filling, special features of clothes (all aspects of communication that constitute features of national culture of the nation and make from the nation the community of people very special and different from other communities and nations. Studying of correctional possibilities of folk games within the frames of playing agendas showed that their psychological and emotional resources provide the conditions for adoption by children the norms of communication.

  18. The Role of Food Selection in Swedish Home Economics: The Educational Visions and Cultural Meaning.

    Science.gov (United States)

    Höijer, Karin; Hjälmeskog, Karin; Fjellström, Christina

    2014-01-01

    This article explores foods talked about and chosen in the education of Swedish Home Economics as a relationship between structural processes and agency. Three data sets from observations and focus group interviews with teachers and students were analyzed for food classifications. These were related to a culinary triangle of contradictions, showing factors of identity, convenience and responsibility. Results show that foods talked about and chosen by teachers and students were reflections of dominant cultural values. Results also indicate that teachers had more agency than students, but that the choices they made were framed by educational visions and cultural values.

  19. The cultural context of development: language as a means for thinking and problem-solving

    Directory of Open Access Journals (Sweden)

    Azwihangwisi Muthivhi

    2011-12-01

    Full Text Available The present study, located in the socio-cultural tradition of research in developmental psychology, uses experimental tasks, adapted from the groundbreaking Lurian study (Luria, 1979, 1976 to investigate South African children’s acquisition and development of thinking and concepts – involving classification and generalisation, and how these concepts are linked to the specific cultural context of their manifestation.The paper provides new ways of understanding possible causes of contemporary problems that children encounter during classroom learning by examining the developmental roots of the specific modes of thinking and concept development in their concrete learning and developmental settings and specific tradition of learning within their schooling.

  20. Meanings of Care: A Culture-Centered Approach to Left-Behind Family Members in the Countryside of China.

    Science.gov (United States)

    Sun, Kang; Dutta, Mohan J

    2016-11-01

    Critical studies of health communication foreground the importance of meanings as organizing frameworks for constituting health. The contested and contradictory meanings articulated around health offer insights into the constraining and enabling roles of structures. Through ethnographic fieldwork conducted in a village in China embedded within the activist framework of the culture-centered approach, this project explores understandings of family care amid left-behind families in rural China against the backdrop of the migration of the middle generation of working adults from families located in rural contexts to cities. We work with the culture-centered approach to explore local meanings of care, understanding everyday care as integral to the health of an aging population that has been erased from the discursive space by market reforms and the hegemonic narrative of national development in China.

  1. The Cultural Meaning of Cardiac Illness and Self-Care Among Lebanese Patients With Coronary Artery Disease.

    Science.gov (United States)

    Dumit, Nuhad Yazbik; Magilvy, Joan Kathy; Afifi, Rima

    2016-07-01

    Cardiac disease is the leading cause of death in Lebanon, accounting for 22% to 26% of total deaths in the country. A thorough understanding of perceptions of cardiac illness and related self-care management is critical to the development of secondary prevention programs that are specific to the Lebanese culture. To explore the cultural perceptions of cardiac illness and the associated meaning of self-care among Lebanese patients. Using a qualitative descriptive method, semistructured interviews were conducted with a purposive sample of 15 Lebanese cardiac patients recruited from a medical center in Beirut, Lebanon. The qualitative descriptive analysis yielded one overarching and two other themes describing perceptions of cardiac illness and self-care within the Lebanese cultural context. The overarching cultural theme was, "Lebanese cardiac patients were unfamiliar with the term concept and meaning of self-care." Lebanese cardiac patients thanked God and accepted their fate (Theme I). The participants considered their cardiac incident a life or death warning (Theme II). Health care providers need to consider patients' cultural perception of illness while planning and evaluating cardiac self-care programs. © The Author(s) 2015.

  2. Embodied Brains, Social Minds, Cultural Meaning: Integrating Neuroscientific and Educational Research on Social-Affective Development

    Science.gov (United States)

    Immordino-Yang, Mary Helen; Gotlieb, Rebecca

    2017-01-01

    Social-affective neuroscience is revealing that human brain development is inherently social--our very nature is organized by nurture. To explore the implications for human development and education, we present a series of interdisciplinary studies documenting individual and cultural variability in the neurobiological correlates of emotional…

  3. Of Models and Meanings: Cultural Resilience in Social–Ecological Systems

    NARCIS (Netherlands)

    Crane, T.A.

    2010-01-01

    Modeling has emerged as a key technology in analysis of social–ecological systems. However, the tendency for modeling to focus on the mechanistic materiality of biophysical systems obscures the diversity of performative social behaviors and normative cultural positions of actors within the modeled

  4. For the Benefit of Others: Generativity and Meaning in Life in the Elderly in Four Cultures

    Czech Academy of Sciences Publication Activity Database

    Hofer, J.; Holger, B.; Au, A.; Poláčková Šolcová, Iva; Tavel, P.; Wong, T.T.

    2014-01-01

    Roč. 29, č. 4 (2014), s. 764-775 ISSN 0882-7974 Institutional support: RVO:68081740 Keywords : Generativity * Belief in the species * Goals * Well-Being * Machiavellianism * Aging * Culture Subject RIV: AN - Psychology Impact factor: 2.646, year: 2014

  5. Librarians and Party Girls: Cultural Studies and the Meaning of the Librarian.

    Science.gov (United States)

    Radford, Marie L.; Radford, Gary P.

    2003-01-01

    Responds to Wayne Wiegand's article that criticizes library and information science by suggesting a cultural studies approach to the field. Applies the work of Stuart Hall to a media stereotype of the female librarian based on the film "Party Girl" to allow new interpretations of media images of the profession. (Author/LRW)

  6. Selective cultures for the isolation of biosurfactant producing bacteria: comparison of different combinations of environmental inocula and hydrophobic carbon sources.

    Science.gov (United States)

    Domingues, Patrícia M; Louvado, António; Oliveira, Vanessa; Coelho, Francisco J C R; Almeida, Adelaide; Gomes, Newton C M; Cunha, Angela

    2013-01-01

    The potential of estuarine microniches as reservoirs of biosurfactant-producing bacteria was evaluated by testing different combinations of inocula and hydrophobic carbon sources. Selective cultures using diesel, petroleum, or paraffin as hydrophobic carbon sources were prepared and inoculated with water from the surface microlayer, bulk sediments, and sediment of the rhizosphere of Halimione portulacoides. These inocula were compared regarding the frequency of biosurfactant-producing strains among selected isolates. The community structure of the selective cultures was profiled using denaturing gradient gel electrophoresis (DGGE) of the 16S rRNA gene fragments at the end of the incubation. The DGGE profiles corresponding to the communities established in selective cultures at the end of the incubation revealed that communities were different in terms of structural diversity. The highest diversity was observed in the selective cultures containing paraffin (H (') = 2.5). Isolates were obtained from the selective cultures (66) and tested for biosurfactant production by the atomized oil assay. Biosurfactant production was detected in 17 isolates identified as Microbacterium, Pseudomonas, Rhodococcus, and Serratia. The combination of estuarine surface microlayer (SML) water as inoculum and diesel as carbon source seems promising for the isolation of surfactant-producing bacteria. Supplemental materials are available for this article. Go to the publisher's online edition of Preparative Biochemistry and Biotechnology to view the supplemental file.

  7. In vitro oocyte culture and somatic cell nuclear transfer used to produce a live-born cloned goat.

    Science.gov (United States)

    Ohkoshi, Katsuhiro; Takahashi, Seiya; Koyama, Shin-Ichiro; Akagi, Satoshi; Adachi, Noritaka; Furusawa, Tadashi; Fujimoto, Jun-Ichiro; Takeda, Kumiko; Kubo, Masanori; Izaike, Yoshiaki; Tokunaga, Tomoyuki

    2003-01-01

    The use of an in vitro culture system was examined for production of somatic cells suitable for nuclear transfer in the goat. Goat cumulus-oocyte complexes were incubated in tissue culture medium TCM-199 supplemented with 10% fetal bovine serum (FBS) for 20 h. In vitro matured (IVM) oocytes were enucleated and used as karyoplast recipients. Donor cells obtained from the anterior pituitary of an adult male were introduced into the perivitelline space of enucleated IVM oocytes and fused by an electrical pulse. Reconstituted oocytes were cultured in chemically defined medium for 9 days. Two hundred and twenty-eight oocytes (70%) were fused with donor cells. After in vitro culture, seven somatic cell nuclear transfer (SCNT) oocytes (3%) developed to the blastocyst stage. SCNT embryos were transferred to the oviducts of recipient females (four 8-cell embryos per female) or uterine horn (two blastocysts per female). One male clone (NT1) was produced at day 153 from an SCNT blastocyst and died 16 days after birth. This study demonstrates that nuclear transferred goat oocytes produced using an in vitro culture system could develop to term and that donor anterior pituitary cells have the developmental potential to produce term offspring. In this study, it suggested that the artificial control of endocrine system in domestic animal might become possible by the genetic modification to anterior pituitary cells.

  8. Integrative studies of cultural evolution: crossing disciplinary boundaries to produce new insights.

    Science.gov (United States)

    Kolodny, Oren; Feldman, Marcus W; Creanza, Nicole

    2018-04-05

    Culture evolves according to dynamics on multiple temporal scales, from individuals' minute-by-minute behaviour to millennia of cultural accumulation that give rise to population-level differences. These dynamics act on a range of entities-including behavioural sequences, ideas and artefacts as well as individuals, populations and whole species-and involve mechanisms at multiple levels, from neurons in brains to inter-population interactions. Studying such complex phenomena requires an integration of perspectives from a diverse array of fields, as well as bridging gaps between traditionally disparate areas of study. In this article, which also serves as an introduction to the current special issue, we highlight some specific respects in which the study of cultural evolution has benefited and should continue to benefit from an integrative approach. We showcase a number of pioneering studies of cultural evolution that bring together numerous disciplines. These studies illustrate the value of perspectives from different fields for understanding cultural evolution, such as cognitive science and neuroanatomy, behavioural ecology, population dynamics, and evolutionary genetics. They also underscore the importance of understanding cultural processes when interpreting research about human genetics, neuroscience, behaviour and evolution.This article is part of the theme issue 'Bridging cultural gaps: interdisciplinary studies in human cultural evolution'. © 2018 The Author(s).

  9. Linking Shared Meaning to Emergent Literacy: Looking through the Lens of Culture

    Science.gov (United States)

    Howes, Carollee; Wishard, Alison Gallwey

    2004-01-01

    A direct pathway to children's literacy forms through the development of shared meaning. Proto-narrative construction and social pretend play with peers can be important tools in children's developing emergent literacy. Early child-care programs provide relatively little unstructured time. To reemphasize shared meaning in the lives of children,…

  10. Isolation of rhamnolipids-producing cultures from faeces: Influence of interspecies communication on the yield of rhamnolipid congeners.

    Science.gov (United States)

    Woźniak-Karczewska, Marta; Myszka, Kamila; Sznajdrowska, Agata; Szulc, Alicja; Zgoła-Grześkowiak, Agnieszka; Ławniczak, Łukasz; Corvini, Philippe F-X; Chrzanowski, Łukasz

    2017-05-25

    The aim of this study was to evaluate the ability of bacterial cultures isolated from cattle, poultry or pig faeces and manure to produce rhamnolipids, as well as to investigate the influence of interspecies communication on possible quantitative differences in the production of rhamnolipid congeners. Initial screening methods (oil spreading, drop collapse, haemolytic activity and emulsification activity) showed that approximately 36% of the 51 isolated cultures exhibited the ability to produce biosurfactants. Subsequent studies using a selected culturable mixed culture (which included Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli) revealed that only P. aeruginosa was able to produce this biosurfactant. HPLC-MS analysis showed that the surface active compounds were rhamnolipids. Further comparative studies confirmed that the total yield of rhamnolipids was notably higher in the bioreactor inoculated with the selected mixed culture (940.58±1.10mg/L) compared to the bioreactor inoculated with the axenic strain of P. aeruginosa (108.47±0.41mg/L). Twelve rhamnolipid congeners were identified during cultivation of the selected mixed culture, whereas six congeners were detected during cultivation of the sole axenic strain of P. aeruginosa. Furthermore, increased production of rhamnolipids was observed when the concentration of autoinducer molecules (AI-2) responsible for interspecies signaling increased, suggesting the influence of quorum-sensing communication on biosynthesis efficiency. This observation may be of importance for large-scale production of this biosurfactant, as it opens new possible solutions based on the use of mixed cultures or external addition of stimulating autoinducers. Copyright © 2016 Elsevier B.V. All rights reserved.

  11. Cultural Considerations: Pharmacological and Nonpharmacological Means for Improving Blood Pressure Control among Hispanic Patients

    Directory of Open Access Journals (Sweden)

    Neela K. Patel

    2012-01-01

    Full Text Available Cardiovascular disease is a leading cause of morbidity and mortality in the United States, and its prevention and treatment remain a priority for the medical community. Ethnic variations account for some differences in the prevalence of hypertension and blood pressure (BP control rates among Hispanics, indicating the need for culturally appropriate management models. Aggressive treatment strategies are key to achieving optimal BP control in high-risk Hispanic patients. Hypertension in this ethnic group continues to be a major health concern. Of note, when provided access to comprehensive care, Hispanics demonstrate similar response rates to treatment as the majority of non-Hispanic whites. This highlights the importance of effective, culturally responsive hypertension management among high-risk Hispanic patients for achieving observable, positive health outcomes.

  12. OVERCOMING THE CULTURAL DIFFERENCES: PARABLE AS A MEANS OF INTERCULTURAL DIALOGUE

    Directory of Open Access Journals (Sweden)

    Tetyana V. Danylova

    2013-09-01

    Full Text Available Purpose. This article attempts to investigate the ways to overcome negative after-effects of intercultural communication. Methodology and theoretical results: To avoid the negative after-effects of intercultural communication caused by the clash of different reality tunnels, it is necessary to go beyond the framework of the specific symbolical territory, which is seen as the only true reality. Expanding the horizons of life, going beyond the boundaries of a personal reality tunnel can be achieved, in particular, by using parables. Acquaintance with parables helps to extend the set of the world perception models and to change the positions of the communication process participants. Parables act as intermediaries between people of different cultures and mentalities. Owing to its archetypical form, parable becomes one of the most popular narratives illustrating deep and multi-faceted truths that sometimes cannot be expressed by any other way. Parable serves as an open and flexible model of the world and a model of an individual "I self". This model helps people to constitute themselves as a part of their world. Parable requires to reject its interpretation in the oppositions inherent in a given culture and to reconsider the very way of understanding. Rejection of the oppositions provides the possibility of perceiving the world as integrity, unity in diversity, helps to overcome one-sided picture of the inner world of a man and his life in society. Understanding of a parable works towards changing personal position, and human creative nature is actualized. Conclusion: Parables uniquely reflect the real world and contribute to personal understanding of reality. Parables are successfully transplanted into different cultures operating as heritage of a culture-recipient and as a connecting link, which optimizes an intercultural dialogue.

  13. Developing Psychological Culture of Schoolchildren as a Means of Supporting Implementation of Basic Education Standards

    OpenAIRE

    Dubrovina I.V.; Lubovsky D.V.,

    2018-01-01

    The paper reviews the social situation of development of children and adolescents in the modern society marked by rapid changes. The development of children and adolescents is described as ‘embedding into the culture’ through the education and is closely associated with the formation of their psychological culture. The paper analyses the conditions of personality development in modern children and adolescents, the factors which impede the communication and understanding of other people; it hi...

  14. Dynamic quantitative analysis of adherent cell cultures by means of lens-free video microscopy

    Science.gov (United States)

    Allier, C.; Vincent, R.; Navarro, F.; Menneteau, M.; Ghenim, L.; Gidrol, X.; Bordy, T.; Hervé, L.; Cioni, O.; Bardin, S.; Bornens, M.; Usson, Y.; Morales, S.

    2018-02-01

    We present our implementation of lens-free video microscopy setup for the monitoring of adherent cell cultures. We use a multi-wavelength LED illumination together with a dedicated holographic reconstruction algorithm that allows for an efficient removal of twin images from the reconstructed phase image for densities up to those of confluent cell cultures (>500 cells/mm2). We thereby demonstrate that lens-free video microscopy, with a large field of view ( 30 mm2) can enable us to capture the images of thousands of cells simultaneously and directly inside the incubator. It is then possible to trace and quantify single cells along several cell cycles. We thus prove that lens-free microscopy is a quantitative phase imaging technique enabling estimation of several metrics at the single cell level as a function of time, for example the area, dry mass, maximum thickness, major axis length and aspect ratio of each cell. Combined with cell tracking, it is then possible to extract important parameters such as the initial cell dry mass (just after cell division), the final cell dry mass (just before cell division), the average cell growth rate, and the cell cycle duration. As an example, we discuss the monitoring of a HeLa cell cultures which provided us with a data-set featuring more than 10 000 cell cycle tracks and more than 2x106 cell morphological measurements in a single time-lapse.

  15. Do I mean what I say and say what I mean? A cross cultural ap-proach to the use of emoticons & emojis in CMC messages

    Directory of Open Access Journals (Sweden)

    Lifen CHENG

    2017-12-01

    Full Text Available The use of emoticons and emojis among online messaging users has achieved a globalized level. This article aims at examining how certain emojis and emoticons are chosen by message senders to regular the intensity of emotions in their messages. Or, by means of using them, message senders wish to blur textual rigidity and show interaction empathy during the communication process. This study analyzes the use of these symbols and graphic elements in message exchange through CMC and SNS, where users from different culture background convey their feeling. For it, two empirical studies of a methodological design were carried out: One pilot study was intended for the research instrument validation. The second was a survey study. By testing online messaging habit, this study enabled the researchers to observe whether the senders inclined to linguistic text use only, or they preferred including an additional emoticon or emoji in their message to communicate their feelings. Results of this research show that, at a certain extent, massage senders’ different culture background could influence on their emotional expressivity level.

  16. BIOCHEMICAL CHARACTERISTICS OF LACTIC ACID PRODUCING BACTERIA AND PREPARATION OF CAMEL MILK CHEESE BY USING STARTER CULTURE

    Directory of Open Access Journals (Sweden)

    T. Ahmed and R. Kanwal

    2004-04-01

    Full Text Available Lactic acid bacteria (LAB were isolated from camel milk by culturing the milk on specific media and pure culture was obtained by sub-culturing. Purification of culture was confirmed by Gram’s staining and identified by different biochemical tests. Camel milk contained lactic acid producing bacteria like Streptococci such as S. cremoris and S. lactis and Lactobacilli such as L. acidophilus. L. acidophilus grew more rapidly in camel milk than others as its growth was supported by camel milk. Ability of each strain was tested to convert lactose of milk into lactic acid. It was observed that 66% lactose was converted by S. lactis 20, whereas S. cremoris 22 and L. acidophilus 23 converted 56 and 74% lactose into lactic acid, respectively. Effect of freeze-drying was also recorded and the results showed that in all cases there was a slight decrease in the cell count before and after the freeze-drying. The decrease was approximately 0.47, 0.078 and 0.86% for S. lactis 20, S. cremoris 22 and L. acidophilus 23, respectively. Starter culture was prepared from strains isolated from camel milk. Camel and buffalo milk cheese was prepared by using starter culture. The strains isolated from camel milk were best for acid production and coagulated the milk in less time. It is concluded that cheese can be prepared successfully from camel milk and better results can be obtained by coagulating milk with starter culture.

  17. Organizational-pedagogic technology of formation of motor functioning culture as mean of physical fitness improvement of 5 form

    Directory of Open Access Journals (Sweden)

    A.H. Deineko

    2015-08-01

    Full Text Available Purpose: to analyze influence of organizational-pedagogic technology of formation of motor functioning’s culture of five form pupils in process of physical education; to test experimentally methodic of formation of motor functioning’s culture by means of general gymnastic in physical education system of comprehensive schools’ pupils. Material: The research was conducted in comprehensive school № 67, Kharkov. 57 pupils of five forms (5-А form - 30 pupils, 5-B - 27 pupils participated in the research. Results: we worked out organizational-pedagogic technology of formation of motor functioning’s culture “Main gymnastic at school”, which positively influenced on development of physical fitness of experimental groups’ pupils. Conclusions: it was established that under influence of selected exercises of main gymnastic and introduced competitiveness elements pupils’ movements became more accurate, plastic, acquired higher amplitude, coordination.

  18. The importance of socio-cultural context for understanding students' meaning making in the study of genetics

    Science.gov (United States)

    Furberg, Anniken; Arnseth, Hans Christian

    2009-03-01

    In this rejoinder to Ann Kindfield and Grady Venville's comments on our article "Reconsidering conceptual change from a socio-cultural perspective: Analyzing students' meaning making in genetics in collaborative learning activities," we elaborate on some of the critical issues they raise. Their comments make apparent some of the crucial differences between a socio-cultural and a socio-cognitive approach towards conceptual change. We have selected some issues that are addressed, either implicitly or explicitly, in their comments. The main issues discussed are talk and interaction as data, the significance of context in interaction studies, the feasibility of generic claims in small-scale interaction studies, and the difference between studying students' understanding of science concepts as opposed to studying the construction of meaning.

  19. Temperature and pH optima of enzyme activities produced by cellulolytic thermophilic fungi in batch and solid-state cultures

    Energy Technology Data Exchange (ETDEWEB)

    Grajek, W

    1986-01-01

    The temperature and pH optima of cellulolytic activities produced by thermophilic fungi in liquid and solid-state cultures were established. Some differences in optimal conditions for enzyme activities, which depended on culture methods, were confirmed. 10 references.

  20. [Does work have a meaning? Basis for a positive work culture].

    Science.gov (United States)

    Sapelli, G

    2010-01-01

    From the anthropological point of view work is the expression and fulfilment of the absolute spirit in the finite sense and is, by its essence, cooperation. This obliges us to reverse the question: "does work have a meaning?", to: "does work have a meaning for workers? Does it allow self-fulfilment?" Work has a meaning if the worker's psychophysical integrity is safeguarded, if by working the individual achieves self-fulfilment as a person, if it has a sense of community. Man as an "actor" achieves self-fulfilment through technical and professional competence, thereby overcoming organizational and social conflicts. Today reducing human and social relations to an economic transaction will lead to the destruction of the meaning of life and work. The study of suicide cases in France Telecom shows that a human being inserted in the working environment wants most of all to obtain recognition. He or she will accept praise or even punishment if this is done with transparency, because the person will acknowledge in such decisions a recognition of his/herself. Many enterprises and consequently many personnel managers do not, in their essence, perceive of any meaning of work, but consider only the instrumental element. The workforce cannot be considered as a commodity, work is embodied in human beings. So either people aim at self-fulfilment or fall into anomie and depression, even to the point of the destructive impulse of elimination. The re-humanization of work and the treatment of enterprises (which are "ill" because they no longer possess any meaning of work) is the challenge that awaits us.

  1. Glycoprotein Ibα receptor instability is associated with loss of quality in platelets produced in culture.

    Science.gov (United States)

    Robert, Amélie; Boyer, Lucie; Pineault, Nicolas

    2011-03-01

    The development of culture processes for hematopoietic progenitors could lead to the development of a complementary source of platelets for therapeutic purposes. However, functional characterization of culture-derived platelets remains limited, which raises some uncertainties about the quality of platelets produced in vitro. The aim of this study was to define the proportion of functional platelets produced in cord blood CD34+ cell cultures. Toward this, the morphological and functional properties of culture-derived platelet-like particles (PLPs) were critically compared to that of blood platelets. Flow cytometry combined with transmission electron microscopy analyses revealed that PLPs formed a more heterogeneous population of platelets at a different stage of maturation than blood platelets. The majority of PLPs harbored the fibrinogen receptor αIIbβ3, but a significant proportion failed to maintain glycoprotein (GP)Ibα surface expression, a component of the vWF receptor essential for platelet functions. Importantly, GPIbα extracellular expression correlated closely with platelet function, as the GPIIb+ GPIbα+ PLP subfraction responded normally to agonist stimulation as evidenced by α-granule release, adhesion, spreading, and aggregation. In contrast, the GPIIb+ GPIbα⁻ subfraction was unresponsive in most functional assays and appeared to be metabolically inactive. The present study confirms that functional platelets can be generated in cord blood CD34+ cell cultures, though these are highly susceptible to ectodomain shedding of receptors associated with loss of function. Optimization of culture conditions to prevent these deleterious effects and to homogenize PLPs is necessary to improve the quality and yields of culture-derived platelets before they can be recognized as a suitable complementary source for therapeutic purposes.

  2. Six cloned calves produced from adult fibroblast cells after long-term culture

    Science.gov (United States)

    Kubota, Chikara; Yamakuchi, Hiroshi; Todoroki, Junichi; Mizoshita, Kazunori; Tabara, Norio; Barber, Michele; Yang, Xiangzhong

    2000-01-01

    Cloning whole animals with somatic cells as parents offers the possibility of targeted genetic manipulations in vitro such as “gene knock-out” by homologous recombination. However, such manipulation requires prolonged culture of nuclear donor cells. Previous successes in cloning have been limited to the use of cells collected either fresh or after short-term culture. Therefore, demonstration of genetic totipotency of cells after prolonged culture is pivotal to combining site-specific genetic manipulations and cloning. Here we report birth of six clones of an aged (17-year-old) Japanese Black Beef bull using ear skin fibroblast cells as nuclear donor cells after up to 3 months of in vitro culture (10–15 passages). We observed higher developmental rates for embryos derived from later passages (10 and 15) as compared with those embryos from an early passage (passage 5). The four surviving clones are now 10–12 months of age and appear normal, similar to their naturally reproduced peers. These data show that fibroblasts of aged animals remain competent for cloning, and prolonged culture does not affect the cloning competence of adult somatic donor cells. PMID:10655472

  3. Using Pure Cultures to Define the Site Preference of Nitrous Oxide Produced by Microbial Nitrification and Denitrification

    Science.gov (United States)

    Sutka, R. L.; Breznak, J. A.; Ostrom, N. E.; Ostrom, P. H.; Gandhi, H.

    2004-12-01

    Defining the site preference of nitrous oxide (N2O) produced in pure culture studies is crucial to interpreting field data. We have previously demonstrated that the intramolecular distribution of nitrogen isotopes (isotopomers) can be used to differentiate N2O produced by nitrifier denitrification and nitrification in cultures of Nitrosomonas europaea. Here, we have expanded on our initial results and evaluated the isotopomeric composition of N2O produced during nitrification and nitrifier denitrification with cultures of Nitrosospira multiformis. In addition, we have analyzed N2O produced during methanotrophic nitrification, denitrification, and fungal denitrification. To evaluate N2O production during nitrification and nitrifier denitrification, we compared the site preference of N2O formed as a result of nitrite reduction and hydroxylamine oxidation with Nitrosomonas europaea and Nitrosospira multiformis. The average site preference of N2O produced by hydroxylamine oxidation was similar for Nitrosomonas europaea (33.0 ± 3.5 ‰ ) and Nitrosospira multiformis (33.1 ± 4.2 ‰ ). Nitrous oxide produced by nitrifier-denitrification by Nitrosomonas europaea and Nitrosospira multiformis had a similar site preference of - 1.4 ± 4.4 ‰ and - 1.1 ± 2.6 ‰ respectively. The results indicate that it is possible to differentiate between N2O produced by nitrite reduction and hydroxylamine oxidation by ammonia oxidizing bacteria. Methanotrophic nitrification was evaluated by analyzing the N2O produced during hydroxylamine oxidation in concentrated cell suspensions of two methane oxidizing bacteria. The site preference of N2O produced by the two methane oxidizers, Methylococcus capsulatus Bath and Methylosinus trichosporium was 31.8 ± 4.7 ‰ and 33.0 ± 4.5 ‰ respectively. The results indicate that a site preference of 33 ‰ is applicable for nitrification regardless of whether a methane oxidizer or ammonia oxidizer is involved in the reaction. To determine the site

  4. Reconsidering Meaning Making and Its Analytical Implications for Cultural Studies of Science Education

    Science.gov (United States)

    Arnseth, Hans Christian; Krange, Ingeborg

    2013-01-01

    The discussion in this forum aims to push some of the ideas further and thus clarify some of the concepts used in the feature article. This includes a debate of the concepts of meaning making, procedural and conceptual learning experiences, the distinction between realism and constructivism in our research practice, and finally how to foster…

  5. Global Design and Local Histories: Culturally Embedded Meaning-Making for Inclusive Education

    Science.gov (United States)

    Mukherjee, Mousumi

    2017-01-01

    This article provides an account of the recent literature on inclusive education, addressing its meaning and significance for school education in postcolonial India. I engage with the major theoretical debates in the academic literature on inclusive education and examine their historical trajectories globally through policy documents. I then…

  6. Pollen and Anther Cultures as Potential Means in Production of Haploid Kenaf (Hibiscus Cannabinus L.)

    OpenAIRE

    Susanto, Dwi; Ibrahim, Ahmed Mahmood; Mat Hussin, Zeti Ermiena Surya

    2013-01-01

    Kenaf (Hibiscus cannabinus L.) is one of the important species of Hibiscus for human needs. It is annual upright plant that produces high biomass, has good adaptability to its environment and inexpensive to be grown. Kenaf has been cultivated in many countries since long time ago mainly for fibers. In Malaysia, however, this plant is considered new and cultivated especially in the eastern parts of Peninsular to replace tobacco plantations that have been no longer supported by the government. ...

  7. Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA

    OpenAIRE

    Vergara,Mauricio; Becerra,Silvana; Díaz-Barrera,Alvaro; Berrios,Julio; Altamirano,Claudia

    2012-01-01

    We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamster ovary cells in batch and semi-perfusion cultures. In batch cultures using glucose as a carbon source, decreasing the temperature caused a reduction in cell growth and an increase in specific pro...

  8. High cell density cultures produced by internal retention: application in continuous ethanol fermentation

    Directory of Open Access Journals (Sweden)

    Berta Carola Pérez

    2004-07-01

    Full Text Available Ethanol has provoked great interest due to its potential as an alternative fuel. Nevertheless, fermentation processes must be developed by increasing the low volumetric productivity achieved in conventional cultures (batch or continuous to make this product become economically competitive. This can be achieved by using techniques leading to high cell concentration and reducing inhibition by the end-product. One of the frequently employed methods involves cell recycling. This work thus developed a membrane reactor incorporating a filtration module with 5 u,m stainless steel tubular units inside a 3L stirred jar fermenter for investigating its application in continuous ethanol production. The effects of cell concentration and transmembrane pressure difference on permeate flux were evaluated for testing the filtration module's performance. The internal cell retention system was operated in Saccharomyces cerevisiae continuous culture derived from sucrose, once fermentation conditions had been selected (30 °C, 1.25 -1.75 vvm, pH 4.5. Filter unit permeability was maintained by applying pulses of air. More than 97% of the grown cells were retained in the fermenter, reaching 51 g/L cell concentration and 8.51 g/L.h average ethanol productivity in culture with internal cell retention; this was twice that obtained in a conventional continuous culture. Key words: Membrane reactor, Saccharomyces cerevisiae, alcoholic fermentation, cell recycling.

  9. Oral fibroblasts produce more HGF and KGF than skin fibroblasts in response to co-culture with keratinocytes

    DEFF Research Database (Denmark)

    Grøn, Birgitte; Stoltze, Kaj; Andersson, Anders

    2002-01-01

    The production of hepatocyte growth factor (HGF) and keratinocyte growth factor (KGF) in subepithelial fibroblasts from buccal mucosa, periodontal ligament, and skin was determined after co-culture with keratinocytes. The purpose was to detect differences between the fibroblast subpopulations...... days by ELISA. When cultured on polystyrene, the constitutive level of KGF and HGF in periodontal fibroblasts was higher than the level in buccal and skin fibroblasts. In the presence of keratinocytes, all three types of fibroblasts in general increased their HGF and KGF production 2-3 times. When...... cells were maintained in collagen, the level of HGF and KGF was decreased mainly in skin cultures. However, in oral fibroblasts, induction after stimulation was at a similar level in collagen compared to on polystyrene. Skin fibroblasts maintained in collagen produced almost no HGF whether...

  10. Analysis of microbial community variation during the mixed culture fermentation of agricultural peel wastes to produce lactic acid.

    Science.gov (United States)

    Liang, Shaobo; Gliniewicz, Karol; Gerritsen, Alida T; McDonald, Armando G

    2016-05-01

    Mixed cultures fermentation can be used to convert organic wastes into various chemicals and fuels. This study examined the fermentation performance of four batch reactors fed with different agricultural (orange, banana, and potato (mechanical and steam)) peel wastes using mixed cultures, and monitored the interval variation of reactor microbial communities with 16S rRNA genes using Illumina sequencing. All four reactors produced similar chemical profile with lactic acid (LA) as dominant compound. Acetic acid and ethanol were also observed with small fractions. The Illumina sequencing results revealed the diversity of microbial community decreased during fermentation and a community of largely lactic acid producing bacteria dominated by species of Lactobacillus developed. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Functional redundancy ensures performance robustness in 3-stage PHA-producing mixed cultures under variable feed operation.

    Science.gov (United States)

    Carvalho, Gilda; Pedras, Inês; Karst, Soren M; Oliveira, Catarina S S; Duque, Anouk F; Nielsen, Per H; Reis, Maria A M

    2018-01-25

    Polyhydroxyalkanoates (PHA) are biopolymers that can be produced by mixed microbial cultures using wastes or industrial by-products, which represent an economical and environmental advantage over pure culture processes. The use of alternate feedstocks enables using seasonal by-products, providing that the process is resilient to transient conditions. The mixed microbial communities of a 3-stage PHA producing system fed initially with molasses and then cheese whey were investigated through amplicon sequencing of the 16S rRNA gene. The transition in feedstock resulted in an adaptation of the acidogenic community, where Actinobacteria dominated with sugarcane molasses (up to 93% of the operational taxonomic units) and Firmicutes, with cheese whey (up to 97%). The resulting fermentation products profile also changed, with a higher fraction of HV precursors obtained with molasses than cheese whey (7.1±0.5 and 1.7±0.7 gCOD/L, respectively). As for the PHA storing culture, the genera Azoarcus, Thauera and Paracoccus were enriched with fermented molasses (average 89% of Bacteria). Later, fermented cheese whey fostered a higher diversity, including some less characterised PHA-storers such as the genera Paenibacillus and Lysinibacillus. Although the microbial community structure was significantly affected by the feedstock shift, the acidogenic and PHA storing performance of the 3-stage system was very similar once a pseudo steady state was attained, showing that a reliable level of functional redundancy was attained in both mixed cultures. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Identity and cultural plurality in spanish textbooks produced in Brazil and selected by PNLD - National Programme of Textbook

    Directory of Open Access Journals (Sweden)

    Jeferson Mundim de Souza

    2016-10-01

    Full Text Available This article aims to promote, given the current reality, in which it sets up a scenario marked by breaking cultural boundaries and understand the world as a more unified universe, reflect on existing conceptions of culture in this context. The world increasingly marked by multicultural societies, the weakening of old cultural references and the multiplication of identity statements. The teaching of foreign languages is an opportunity to place the student in planetary universe of an apparent online culture, but which increasingly reinforces different identities. In this sense, the article presented here, and promote space for a discussion of these very contemporary issues, presents the analysis of a survey of two of the four textbooks in Spanish, selected to join the National Book Program for Education East, delimiting the choice of the characteristics of space and time for analysis and construction of this writing: El arte de leer Spanish and Síntesis: course española language. Not the total analysis of the books will be presented, opting to make a cut, taking as a criterion some teaching units and activities more targeted to the theme proposed, in addition to meet the rules and brevity of this article. Such research revolved around the following questions: How do the textbooks for the teaching and learning of Spanish produced in Brazil include: a the cultural diversity of the Hispanic world? ; b the claim - with respect to their community in training and socializing (s of identity (s American latina- (s? ; and c interaction - proposal from learning strategies, with the identity formation of the Brazilian student? Therefore, we adopted the methodology of qualitative analysis and content analysis technique, taking as indicators the following categories: geographical areas and cultural areas explored in the texts; the identities of anonymous characters and famous circulating in the books; and educational activities related to cultural issues

  13. ISOLATION, CHARACTERIZATION MORPHOLOGICAL AND EVALUATION OF MYCELIAL GROWTH AND SPORULATION OF VARIOUS MEANS CULTURE OF Quambalaria sp.

    Directory of Open Access Journals (Sweden)

    Ediellen Mayara Corrêa Gomes

    2016-12-01

    Full Text Available The characterization of microorganisms is strategic for plant protection. The objective was to analyze the morphological characteristics and the mycelial growth and sporulation on different culture media fungus Quambalaria sp. Morphological characterization was based on color, type of colonies, type and size of spores were used taxonomic keys and specialized literature for description and identification of fungal structures. For the assessment of mycelial growth and sporulation were used means potato dextrose agar culture (PDA, vegetable-agar (V8 agar and oatmeal agar (OA. two isolates were obtained denominated Q1 and Q2 showed that different types of colonies and mycelial growth, and the spores of both characterized as hyaline, miniature format with obovoid the fusiform and its chambered hyphae. A linear relationship between sporulation and mycelial growth was observed, that is, the larger the sporulation, the greater mycelial growth. The means of V8-agar culture was the most stimulated mycelial growth and sporulation isolated Q1 and the BDA through the isolated Q2, with the MIGS of 11.07 and 10.57 mm day-1, respectively. The results of this study allow us to base the start of a study on the Quambalaria gender, in northern Brazil and to add to the few reported studies on this pathosystem. Keywords: leaf spot; eucalyptus; phytopathogen; forest pathology.

  14. Making Sense of HIV in Southeastern Nigeria Fictional Narratives, Cultural Meanings, and Methodologies in Medical Anthropology

    Science.gov (United States)

    Winskell, Kate; Brown, Peter J.; Patterson, Amy E.; Burkot, Camilla; Mbakwem, Benjamin C.

    2018-01-01

    Fictional narratives have rarely been used in medical anthropological research. This article illustrates the value of such narratives by examining how young people in southeastern Nigeria navigate the cultural resources available to them to make sense of HIV in their creative writing. Using thematic data analysis and narrative-based methodologies, it analyzes a sample (N = 120) from 1,849 narratives submitted by Nigerian youth to the 2005 Scenarios from Africa scriptwriting contest on the theme of HIV. The narratives are characterized by five salient themes: tragedy arising from the incompatibility of sex outside marriage and kinship obligations; female vulnerability and blame; peer pressure and moral ambivalence; conservative Christian sexual morality; and the social and family consequences of HIV. We consider the strengths and limitations of this narrative approach from a theoretical perspective and by juxtaposing our findings with those generated by Daniel Jordan Smith using standard ethnographic research methods with a similar Igbo youth population. [HIV, Igbo, youth, narrative, methodology] PMID:23804317

  15. [The meaning of autonomy in Chinese culture: obtaining informed consent for operation].

    Science.gov (United States)

    Lin, Mei-Ling; Wu, Jo Yung-Wei; Huang, Mei-Chih

    2008-10-01

    The purpose of gaining the patient's informed consent is ethical, lying in respect for his or her autonomy, and such consent forms the foundation for the performance of clinical medical treatment. In order to respect the patient's autonomy, for example, during decisions about operations, doctors have the obligation to clearly explain that patient's medical condition to him/her. A thorough briefing should be given prior to the obtaining of the patients' consent. In fulfillment of their duties as medical professionals, both doctors and nurses should be involved in clinically informing patients as well as in obtaining their signature for operation and anesthesia. Although informing patients about their physical state is not the responsibility of nurses, it remains absolutely necessary for nurses to understand how people in Asian cultures understand autonomy. This paper begins with a discussion of autonomy in ethics, and then outlines the differences between the Eastern and Western concepts of autonomy, before discussing the obtaining of the signature of consent, a process performed by the nursing staff during clinical treatment, and resulting in the provision of such signatures by patients with the legal capacity to provide them.

  16. Call for papers-special issue on Europe’s culture(s): Negotiating cultural meanings, values, and identities in the European context

    OpenAIRE

    Benet-Martinez, V; Mesquita, Batja; Uskul, A; Gobel, M

    2016-01-01

    Europe has recently been facing several unprecedented and deeply significant challenges to its identity: From the Charlie Hebdo murders and Paris shootings in France to the PEGIDA discussions in Germany, from the possibility of Grexit to the UK discussions to leave the EU, and from pro-independence movements within Spain and the UK to other countries working on reunification (Cyprus), and perhaps most importantly the current refugee crisis. In all these cases, the meaning(s) of European cultu...

  17. An assessment of whole effluent toxicity testing as a means of regulating waters produced by the oil and gas industry

    International Nuclear Information System (INIS)

    Hill, S.L.; Bergman, H.L.

    1993-01-01

    Approximately 500 million barrels of produced water are discharged to Wyoming's surface waters by the oil and gas industry. This discharges are of two types: direct and indirect. The direct discharges have been issued NPDES permits requiring whole effluent toxicity testing. Toxicity testing requirements have not been incorporated into permits written for indirect discharges because of the applicability of toxicity testing for regulating these waters has not been determined. Preliminary testing has shown that most produced waters are toxic at the point of discharge because of high concentrations of hydrogen sulfide, but that the toxicity of an indirect discharge is often lost before it reaches a receiving stream. Thus, whole effluent toxicity testing of an indirect discharge may be overly stringent, resulting in treatment or reinjection of the water or closure of the well. Any of these options would have severe economic consequences for oil producers and the state's agricultural industry. The purpose of this study was to determine whether whole effluent toxicity testing actually predicts the in-stream effects of indirect discharges on water quality and benthic invertebrate populations. The authors will report the results of short-term ambient toxicity tests and in-stream bioassessments performed upstream and downstream of six indirect discharges located in four drainages in Wyoming

  18. Therapeutic potential of secondary metabolites produced in the hairy roots cultures

    Directory of Open Access Journals (Sweden)

    Tomasz Kowalczyk

    2015-05-01

    Full Text Available Plants have always been a source of many valuable substances for humans. Growing advancement of methods of modern biotechnology, combined with genetic engineering techniques, gradually increase the variety of compounds obtained, the number of plant species used and the production efficiency. Consequently, there is an undebatable interest in biotechnological production of such compounds, especially those pharmacologically active, that can be used in treatment of neoplastic, viral, and many other types of diseases. Most of these compounds represent a diverse group of secondary metabolites. One of the effective ways of obtaining such molecules is the utilization of hairy roots cultures. The advantages of such systems make them an attractive method of obtaining important plant-derived compounds, creating an interesting alternative to other methods, including the cell suspension cultures or expensive chemical syntheses.

  19. Strontium ranelate changes the composition and crystal structure of the biological bone-like apatite produced in osteoblast cell cultures.

    Science.gov (United States)

    Querido, William; Campos, Andrea P C; Martins Ferreira, Erlon H; San Gil, Rosane A S; Rossi, Alexandre M; Farina, Marcos

    2014-09-01

    We evaluate the effects of strontium ranelate on the composition and crystal structure of the biological bone-like apatite produced in osteoblast cell cultures, a system that gave us the advantage of obtaining mineral samples produced exclusively during treatment. Cells were treated with strontium ranelate at concentrations of 0.05 and 0.5 mM Sr(2+). Mineral substances were isolated and analyzed by using a combination of methods: Fourier transform infrared spectroscopy, solid-state (1)H nuclear magnetic resonance, X-ray diffraction, micro-Raman spectroscopy and energy dispersive X-ray spectroscopy. The minerals produced in all cell cultures were typical bone-like apatites. No changes occurred in the local structural order or crystal size of the minerals. However, we noticed several relevant changes in the mineral produced under 0.5 mM Sr(2+): (1) increase in type-B CO3 (2-) substitutions, which often lead to the creation of vacancies in Ca(2+) and OH(-) sites; (2) incorporation of Sr(2+) by substituting slightly less than 10 % of Ca(2+) in the apatite crystal lattice, resulting in an increase in both lattice parameters a and c; (3) change in the PO4 (3-) environments, possibly because of the expansion of the lattice; (4) the Ca/P ratio of this mineral was reduced, but its (Ca+Sr)/P ratio was the same as that of the control, indicating that its overall cation/P ratio was preserved. Thus, strontium ranelate changes the composition and crystal structure of the biological bone-like apatite produced in osteoblast cell cultures.

  20. Community health workers as cultural producers in addressing gender-based violence in rural South Africa.

    Science.gov (United States)

    de Lange, Naydene; Mitchell, Claudia

    2016-01-01

    South Africa has been experiencing an epidemic of gender-based violence (GBV) for a long time and in some rural communities health workers, who are trained to care for those infected with HIV, are positioned at the forefront of addressing this problem, often without the necessary support. In this article, we pose the question: How might cultural production through media making with community health workers (CHWs) contribute to taking action to address GBV and contribute to social change in a rural community? This qualitative participatory arts-based study with five female CHWs working from a clinic in a rural district of South Africa is positioned as critical research, using photographs in the production of media posters. We offer a close reading of the data and its production and discuss three data moments: CHWs drawing on insider cultural knowledge; CHWs constructing messages; and CHWs taking action. In our discussion, we take up the issue of cultural production and then offer concluding thoughts on 'beyond engagement' when the researchers leave the community.

  1. Contribution of natural milk culture to microbiota, safety and hygiene of raw milk cheese produced in alpine malga

    Directory of Open Access Journals (Sweden)

    Rosaria Lucchini

    2018-04-01

    Full Text Available Processing of alpine milk in malga farms is carried out under conditions that can favor contamination by coliforms, coagulase-positive staphylococci, or pathogens such as Listeria monocytogenes. With the aim to improve the hygienic characteristics and safety of cheese produced in four malga farms the use of lyophilized Natural Milk Culture prepared with selected strains was tested. Two cheesemaking tests were carried out in the same day always starting from the same milk: in the first case following the malga recipe that uses either Natural Whey Culture or without the addition of a starter, in the second one using a Natural Milk Culture. Cheesemaking were carried out in four malga farms located in the west area of Trentino region within the same week. For hygienic and safety evaluation, aerobic colony count, coagulase-positive staphylococci, Escherichia coli, staphylococcal toxins, Listeria monocytogenes , and Salmonella spp, pH and aw were determined in raw milk from evening and morning milking, curd in vat, curd after extraction and two months-ripened cheese. Pathogens or toxins, high values of coagulase- positive staphylococci and E. coli were not found in cheese samples. However, in the curd coagulase-positive staphylococci reached values almost of 5 Log CFU/g in the two malga without starter cultures. The use of Natural Milk Culture reduced E. coli counts. In addition, DNA was extracted from cheese samples and from Natural Milk Culture and the composition of the microbial community determined by Next Generation Sequencing method. The determination of cheese microbial communities demonstrated that the use of Natural Milk Culture exerted different effects in the different malga, in any case preserving bacterial biodiversity.

  2. Controlling of processes for producing energy carriers by means of biotechnique. Reglering av processer foer att producera energibaerare med bioteknik

    Energy Technology Data Exchange (ETDEWEB)

    Mandenius, C F

    1987-01-01

    The most suitable parameter to reducing production cost is controlling of sugar content when converting sulfite lye or cellulose hydrolysate to ethanol. By keeping the sugar content low in the end product the yield and productivity are improved. A sensor for ethanol in the yeast tank has been developed and the continous analysis of glucose has been performed by means of an analyser for enzymes.

  3. Representing and Coping with Early Twentieth-Century Chongqing: “Guide Songs” as Maps, Memory Cells, and Means of Creating Cultural Imagery

    Directory of Open Access Journals (Sweden)

    Igor Chabrowski

    2013-03-01

    Full Text Available Chongqing’s “guide songs” form an interesting subgenre among the broad category of haozi 號子 (workers’ songs. These early twentieth-century songs were a form of rhythm-based oral narrative describing Chongqing’s urban spaces, river docks, and harbors. Each toponym mentioned in the lyrics was followed by a depiction of the characteristic associations, whether visible or symbolic, of the place. This article aims to analyze the verbal images of Chongqing presented in these songs in order to understand how the city was remembered, reproduced, and represented. The article deconstructs representations of the city produced by the lower classes, mainly by Sichuan boatmen, and links culturally meaningful images of urban spaces with the historical experiences of work, religion, and historical-mythical memory. It also points to the functions that oral narratives had in the urban environment of early twentieth-century Chongqing. Rhythmic and easy to remember, the songs provided ready-to-use guides and repositories of knowledge useful to anyone living or working there. A cross between utilitarian resource books and cultural representations, they shaped modes of thinking and visualizations of urban spaces and Chongqing. Finally, this article responds to the need to employ popular culture in our thinking about Chinese cities and the multiplicity of meanings they were given in pre-Communist times.

  4. Producing Films, Producing Meanings: Some Theoretical Reflections

    Directory of Open Access Journals (Sweden)

    Giorgio Grignaffini

    2015-01-01

    Full Text Available L'articolo si pone l'obiettivo di stimolare una maggiore attenzione da parte della semiotica del cinema alle pratiche produttive e ai materiali preparatori del film, finora poco studiati rispetto alle dinamiche che i testi stessi attivano sulla ricezione. Tale proposta di allargamento del campo di ricerca, viene approfondita nelle sue premesse teoriche in due momenti. Dapprima viene contestualizzata rispetto alla pratica dell'analisi semiotica "tradizionale"; poi, utilizzando gli strumenti della sociosemiotica elaborata da Eric Landowski, in particolare sfruttando il concetto di "aggiustamento" sviluppato nel quadro della riflessione sulle modalità di interazione, vengono evidenziati campi di applicazione (ad es. il lavoro del regista con l’attore e possibili vantaggi euristici.

  5. Cytogenetic studies on stevia rebaudiana produced by tissue culture and affected by gamma rays and drought

    International Nuclear Information System (INIS)

    Awad, A.S.A

    2009-01-01

    The present investigation was under taken to carry out in the laboratories of the Natural Products Department, National Center for Radiation Research and Technology, Atomic Energy authority, Nasr city, Cairo, Egypt, to study the effect of gamma radiation doses, osmostress and the combined effects between them on tissue culture, some biochemical analysis and molecular genetic marker in stevia rebaudiana bertoni. The results obtained were: Tissue culture 1- micropropagation media: stevia rebaudiana plantlets cultured on MS medium hormones free for micropropagation.Hormones such as BAP and NAA with different concentrations induced callus formation and give slight growth.Study the effect of gamma radiation, osmostress and the combined effects between them : 1)The effect of gamma radiation on buds survival: Gamma radiation doses (10, 20 and 30 Gy) induced decreasing in bud survival percentage with increasing radiation dose in stevia rebaudiana. The dose 30 Gy was induced 60% mortality.2) Study the effect of gamma radiation on some biochemical analysis: Gamma radiation doses induced increase in the total carbohydrate with doses (20 and 30 Gy) but decreased with dose 10 Gy. Proline contents increased in plantlets with increasing doses . The total protein was increased with doses (10 and 20 Gy), but the dose 30 Gy induced decrease in total protein. Gamma radiation doses induced decreasing in total DNA while, the nucleic acid RNA increased.3) The effect of osmostress on buds survival: The concentrations (40000,50000,60000,70000 and 80000 ppm) from sucrose or sorbitol decreased the bud survival and shoot length in stevia plantlets with increasing sucrose or sorbitol levels. 4) The effect of osmostress on some biochemical analysis: Sucrose and sorbitol concentrations (40000,50000,60000,70000 and 80000 ppm) caused decrease in total carbohydrate.

  6. Beliefs about history, the meaning of historical events and culture of war

    Directory of Open Access Journals (Sweden)

    Magdalena Bobowik

    2010-06-01

    Full Text Available This study examines beliefs concerning the content of history, the meaning of Second World War (WWII and the evaluation of historical events in relation to pro-war attitudes. Participants were 1183 university students from Spain, Portugal, Argentina, Brazil, Peru and Cape Verde. Four supra-level dimensions in the representations of the past were found: History as progress and leaders-oriented, history as focused on justifying calamities, history as violence and catastrophe, and history as meaningless. The prevalent positive beliefs about history were linked with enthusiasm to fight in a future war for one’s country.---Se estudiaron las creencias sobre el contenido de la historia, el significado de la Segunda Guerra Mundial y la evaluación de eventos históricos en relación con una actitud favorable a la guerra. Los participantes fueron 1183 estudiantes universitarios de España, Portugal, Argentina, Brasil, Perú y Cabo Verde. Se encontraron cuatro grandes dimensiones en las representaciones sobre el pasado: la historia como proceso de progreso y dirigida por líderes; la historia compuesta por calamidades que se deben aceptar; la historia como violencia y catástrofes; y, la historia como carente de sentido. La prevalente visión positiva de la historia se asoció a una actitud favorable a luchar en una nueva guerra.

  7. Cultural and Mathematical Meanings of Regular Octagons in Mesopotamia: Examining Islamic Art Designs

    Directory of Open Access Journals (Sweden)

    Jeanam Park

    2018-03-01

    Full Text Available The most common regular polygon in Islamic art design is the octagon. Historical evidence of the use of an 8-star polygon and an 8-fold rosette dates back to Jemdet Nasr (3100-2900 B.C. in Mesopotamia. Additionally, in ancient Egypt, octagons can be found in mathematical problem (Ahmose papyrus, Problem number 48, household goods (papyrus storage, architecture (granite columns and decorations (palace decorations. The regular octagon which is a fundamentally important element of Islamic art design, is widely used as arithmetic objects in metric algebra along with other regular polygons in Mesopotamia. The 8-point star polygon has long been a symbol of the ancient Sumerian goddess Inanna and her East Semitic counterpart Ishtar. During the Neo-Assyrian period, the 8-fold rosette occasionally replaced the star as the symbol of Ishtar. In this paper, we discuss how octagonal design prevailed in the Islamic region since the late ninth century, and has existed in Mesopotamia from Jemdet Nasr to the end of third century B.C. We describe reasons why the geometric pattern of regular polygons, including regular octagons, developed in the Islamic world. Furthermore, we also discuss mathematical meanings of regular polygons.

  8. Gene probes to detect cross-culture contamination in hormone producing cell lines

    DEFF Research Database (Denmark)

    Matsuba, I; Lernmark, A; Madsen, Ole Dragsbæk

    1988-01-01

    hamster insulin gene. Karyotyping confirmed the absence of human chromosomes in the Clone-16 cells while sizes, centromere indices, and banding patterns were identical to Syrian hamster fibroblasts. We conclude that the insulin-producing Clone-16 cells are of Syrian hamster origin and demonstrate...

  9. Characterization of aerosols produced in cutting steel components and concrete structures by means of a laser beam

    International Nuclear Information System (INIS)

    Tarroni, G.; Melandri, C.; Zaiacomo, T. de; Lombard, C.C.; Formignani, M.

    1986-01-01

    The technique of cutting based on the use of a laser beam is studied as a possible method in nuclear plant dismantling (OECD, 1982). The technique implies a relevant problem of contamination due to high aerosol production. Tests have been carried out to characterize the aerosol produced in cutting steel and concrete in terms of size spectrum, electric charge and chemical composition in comparison with bulk material composition. The high temperature value locally reached in the cutting zone causes material vaporization with emission of very fine primary particles. In such conditions aerosol coagulation is very fast (it occurs in less than 1s) and leads to aggregates. Research has been aimed at finding the characteristics of the aerosol removable from the cutting zone by ventilation and evaluating the morphology of the particles that diffuse at approximately 50 cm from the generation point, or settle on the cutting-box base. (author)

  10. A cultural take on the links between religiosity, identity, and meaning in life in religious emerging adults.

    Science.gov (United States)

    Negru-Subtirica, Oana; Tiganasu, Alexandra; Dezutter, Jessie; Luyckx, Koen

    2017-03-01

    Identity and meaning in life are core developmental assets in emerging adulthood. We analysed how religiosity is related to these intentional strivings in emerging adults enrolled in theological education, by depicting (1) identity strivings and meaning in life accounts in faith narratives (Study 1) and (2) links between personal identity and meaning in life profiles and religious beliefs, behaviours, and subjective experiences (Study 2). Both studies highlighted that a Foreclosed status, with high personal commitment and reduced exploration, was dominant in faith narratives and personal identity profiles. Also, in narratives meaning in life was reflected by a strong focus on presence of meaning through religious insights. Nonetheless, global meaning in life profiles indicated that many emerging adults were searching for a meaning in their lives, while reporting lower levels of presence of meaning. Identity Achievement and High Presence-High Search profiles were linked to the highest levels of subjective, behavioural, and cognitive religiosity. We highlighted the multidimensionality of identity and meaning in life strivings in emerging adults attending theological schools. We pointed out that even in a somewhat foreclosed cultural context (e.g., Romanian Christian Orthodox theological schools), religion represents a dynamic social and ideological context for self-development. Statement of contribution What is already known on this subject? Religious beliefs increase in emerging adults, doubled by decreases in religious behaviours, linked to an adherence to a more personal approach to religion. Religious youth are more committed to their faith and also explore identity and life meaning in relation to their religious strivings. Youth religious exemplars report close links between their religious faith and strivings for meaningful life goals. What does this study add? We investigated Christian Orthodox theology students, for whom religion is a normative dimension of

  11. Availability of pearl producing marine bivalves in south-eastern coast of Bangladesh and culture potentialities

    Directory of Open Access Journals (Sweden)

    Md Ataur Rahman

    2015-12-01

    Full Text Available The present study was conducted during September 2014 to July 2015 to identify the pearl bearing bivalves in south-eastern coast of Bangladesh and culture potentialities of marine oyster in captivity based on field investigation. A total of 7 pearl bearing bivalve species were identified in the coast with a salinity of 18-34 ppt, pH 8.1-8.3 and water depth ranged 0.2-2.0 meter in their habitat. From the collected bivalves, most abundant oyster species windowpane oyster, Placuna placenta (Linnaeus, 1758 was reared in fiber glass tanks with seawater for a period of 6 months. During rearing highest survival rate of 88% was observed in T1 with sandy and gravel substratum and lowest survival rate of 78% was found in T2 with muddy substratum. Average temperature and salinity were varied between 24 °C-25 °C and 21-26 ppt respectively. From the reared oyster, highest 54 nos. small pearls in the month of April and lowest 7 pearls in December from a single P. placenta were obtained. The study proved that pearls can be obtained from the marine oysters in captivity in Bangladesh, and this offers large scale culture potentialities in our coast.

  12. The examination of Hevea brasiliensis plants produced by in vitro culture and mutagenesis by DNA fingerprinting techniques

    International Nuclear Information System (INIS)

    Low, F.C.; Atan, S.; Jaafar, H.

    1998-01-01

    Rubber (Hevea brasiliensis) plants derived from anther and ovule culture as well as gamma-irradiated plants were examined by several DNA marker techniques. These include restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNA (RAPD), sequence tagged microsatellite sites (STMS), DNA amplification fingerprinting (DAF) and amplified fragment length polymorphisms (AFLPs). Compared to control plants produced by vegetative propagation (cutting and budding), plants produced by in vitro culture appeared to have a reduction in the number of rDNA loci. Two RAPD protocols were compared and found to be similar in amplification of the major DNA bands. After confirmation that the RAPD method adopted was reproducible, the technique was applied to the present studies. Eight out of the 60 primers screened were able to elicit polymorphisms between pooled DNA from in vitro culture plants. Variations in DNA patterns were observed between pooled DNA samples of anther-derived plants as well as between anther-derived and ovule-derived plants. Comparisons of RAPD patterns obtained between anther-derived plants exposed to increasing dosages of gamma-irradiation with non irradiated anther-derived plants revealed distinct DNA polymorphisms. The changes in DNA profiles did not appear to be correlated to the dosage of irradiation. Since somaclonal variation was detected, it was difficult to identify changes which were specifically caused by irradiation. Application of the STMS technique to tag micro satellite sequences (GA) n , (TA) n and (TTA) n in the hydroxymethylglutaryl coenzyme A reductase-1 (hmgr-1) gene failed to detect differences between plants derived from anther and ovule culture. Although restriction endonuclease digestions with methylation sensitive enzymes suggested that four in vitro culture plants examined exhibited similar digestion patterns as the controls, a change in cytosine methylation in one anther-derived plant was detected. Examination of

  13. Importance in dairy technology of bacteriocins produced by dairy starter cultures

    Directory of Open Access Journals (Sweden)

    Bedia Şimşek

    2002-03-01

    Full Text Available Bacteriocins produced by Lactic acid bacteria (LAB and propionic acid bacteria (PAB are heterogeneous group of peptide inhibitors which include lantibiotics (class I, e. g. nisin, small heat-stable peptides (class II, e. g. pediocin PA-1 and large heat-labile proteins (class III, e. g. helveticin J. Many bacteriocins belonging to the first two groups can be successfully used to inhibit undesirable microorganisms in foods, but only nisin is produced industrially and is used as a food preservative. LAB and PAB develops easily in milk and milk products. LAB and PAB growth in dairy products can cause microbial interference to spoilage and pathogenic bacteria through several metabolits, specially bacteriocins. The review deals with the description of milk-borne bacteriocins and their application in milk and milk products either to extend the shelf life or to inhibit milk pathogens.

  14. Making Material and Cultural Connections: the fluid meaning of ‘Living Electrically’ in Japan and Canada 1920–1960

    Directory of Open Access Journals (Sweden)

    Heather Chappells

    2018-05-01

    Full Text Available This article explores how material and cultural ‘connections’ to households were crucial in defining historical pathways of domestic electrification in Canada and Japan. Though relatively overlooked in the history of energy, we contend that the intricacies of household connections are important in understanding convergence and divergence in electrified ways of life. Electricity’s arrival in urban and rural homes was an uneven process in part due to geographical diversity and the variable connection policies of public or private providers. Selective insights from our two countries show that spatial irregularities in grid expansion and social inequities in the meaning of electrical service further arose out of misalignments between developers’ visions and the diverse material cultures of households. This disparity manifested in the form of disconnections between non-standard wiring and equipment, as well as in the form of consumer resistance, protests and even illicit acts as people fought to get the electrical service they desired. Instead of seeing electrification as a single, universal ideal around which all countries eventually converged, we conclude that it has always been an open-ended, negotiable and culturally fluid process.

  15. The study by means of a photomultiplier of the scintillations produced by α particles striking a zinc sulphide screen

    International Nuclear Information System (INIS)

    Anthony, J.P.

    1955-06-01

    The object of the study is the accurate counting of α particles by p-m. detection of their scintillations upon impact with a zinc sulphide screen. The main advantage of the method is the extreme simplicity of the electronics used: the possibility of obtaining a utilizable pulse from the p-m. (EMI5311) without any amplification, and in linear response, is demonstrated. The scintillation produced by an impact on Zn-S has also been studied experimentally. The decrease of light intensity in relation to time may be interpreted by the exponential relation: I = I 0 exp (-t / τ) whereby τ = (39 ± 0,1) 10 -6 s. The relation between scintillation intensity and remaining trajectory after travel through a given air-space has also been determined. Possible suitable applications of this method of α counting are those where good stability and low background are necessary. Results stated bear on air contamination studies, isotopic composition variation measurement of uranium, bismuth content measurement in alloys by irradiation of specimens in a thermal neutron flux and α count on the Po formed. (author) [fr

  16. A Rapid Culture Technique Produces Functional Dendritic-Like Cells from Human Acute Myeloid Leukemia Cell Lines

    Directory of Open Access Journals (Sweden)

    Jian Ning

    2011-01-01

    Full Text Available Most anti-cancer immunotherapeutic strategies involving dendritic cells (DC as vaccines rely upon the adoptive transfer of DC loaded with exogenous tumour-peptides. This study utilized human acute myeloid leukemia (AML cells as progenitors from which functional dendritic-like antigen presenting cells (DLC were generated, that constitutively express tumour antigens for recognition by CD8+ T cells. DLC were generated from AML cell lines KG-1 and MUTZ-3 using rapid culture techniques and appropriate cytokines. DLC were evaluated for their cell-surface phenotype, antigen uptake and ability to stimulate allogeneic responder cell proliferation, and production of IFN-γ; compared with DC derived from normal human PBMC donors. KG-1 and MUTZ-3 DLC increased expression of CD80, CD83, CD86, and HLA-DR, and MUTZ-3 DLC downregulated CD14 and expressed CD1a. Importantly, both KG-1 and MUTZ-3-derived DLC promoted proliferation of allogeneic responder cells more efficiently than unmodified cells; neither cells incorporated FITC-labeled dextran, but both stimulated IFN-γ production from responding allogeneic CD8+ T cells. Control DC produced from PBMC using the FastDC culture also expressed high levels of critical cell surface ligands and demonstrated good APC function. This paper indicates that functional DLC can be cultured from the AML cell lines KG-1 and MUTZ-3, and FastDC culture generates functional KG-1 DLC.

  17. A case study of healthcare professional views on the meaning of data produced by hand hygiene auditing.

    Science.gov (United States)

    Dawson, Carolyn H

    2015-11-01

    Measurement of hand hygiene (HH), crucial for patient safety, has acknowledged flaws stemming from methods available. Even direct observation, the World Health Organization gold standard, may lead to behaviour changes which can affect outcome validity. However, it remains important to understand current levels of HH to allow targeted interventions to be developed. This has resulted in wider adoption of auditing processes. This study addressed how healthcare professionals perceive data generated by HH auditing processes. Qualitative study involving participatory observation and semi-structured interviews with 30 healthcare professionals recruited from a large National Health Service (NHS) two-hospital site in England. Healthcare professionals perceived two main problems with HH measurement, both associated with feedback: (1) lack of clarity with regard to feedback; and (2) lack of association between training and measurement. In addition, concerns about data accuracy led the majority of participants (22/30) to conclude audit feedback is often 'meaningless'. Healthcare professionals require meaningful data on compliance with HH to engender change, as part of a multimodal strategy. Currently healthcare professionals perceive that data lack meaning, and are not seen as drivers to improve HH performance. Potential opportunities to change practice and improve HH are being missed.

  18. Isolation and Characterization of an Amylase Producing Yeast and its Application in Carotenoid Production Using Dual Culture

    Directory of Open Access Journals (Sweden)

    Iraj Nahvi

    2005-06-01

    Full Text Available Starch is a plant polysaccharide with unique applications in Iran. Its increasing production and processing recently have led to large volumes of industrial effluent as an environmental pollutant. In this study, an amylase producing yeast is isolated and identified as “Cryptococcus aerius” to investigate some of its characteristics such as its amylase secretion and starch digesting patterns, kinetics of amylase complex, and its capability for carotenoid production in dual culture. The results indicate that C.aerius is capable of soluble and raw maize starch digestion and assimilation. Raw starch digestion is scarce among yeast species; hence, it is industrially important. C.aerius digests soluble starch in the first 10 hours of cultivation and on the basis of amylase secreting patterns, it is therefore categorized with fast growing species on starch as carbon source. Non-pathogenicity, digestion of raw starch, heat stability of the secreted amylases complex (>55˚C, and the optimum pH level of 5.5- 6 for amylases complex are the set of properties that make this species capable of use in microbial production on an industrial scale. Absorption of carotenoid extract obtained from dual fermentation of C.aerius and Rhodotorula sp. indicates that the quality of carotenoids produced in dual fermentation is the same as that produced from pure Rhodotorula sp culture.

  19. Cytogenetic Studies on Sativa Rebounded Produced by Tissue Culture and Affected by GAMMA Rays and Drought

    International Nuclear Information System (INIS)

    Awad, A.S.A.

    2009-01-01

    Stevia rebaudiana Bertoni is a plant which produces a variety of high-potency, low-calorie sweetener in its leaf tissue (Jarma et al ., 2006). The leaves of this plant contain sweet diterpene glucosides; rebaudioside A, rebaudioside C, stevioside and dulcoside. Stevioside is about 110 to 270 times sweeter than sucrose, while rebaudioside A is 150 to 320 times sweeter than sucrose (Yao et al., 1999). The leaves also produce biologically active substances, e.g. flavonoids, coumarins, cinnamic acids and essential oil (Dzyuba, 1998). (Lobov and Yurtaeva, 2002) showed that diterpenoid glycosides from leaves of S. rebaudiana were the most promising non-sugar sweeteners of plant origin for food and pharmaceutical industries to overcome the problem of human diseases related to disorders of carbon metabolism. The sweetener from leaves has a good taste and is suitable for use in food products as chocolates, marmalades, biscuits, ice-cream, sweets, juices, beverages and candy. The dried leaves could be mixed within the tea packages to reduce the consumption of sugar. The stevioside does not induce tooth decay could safely by used by diabetic patients and could be used in the low caloric diets to reduce human body weight without side effects for these reasons many countries are now using this plant to produce a larger portion of their sugar consumption (El-Zifzafi, 2003). Stevia, Stevia rebaudiana Bertoni is a small herbaceous plant (2 n = 22). It is a member of compositae family (Yao et al., 1999). Estimates of total number of species in this genus ranges from 150 to 300 . Stevia rebaudiana is one of the species of the genus stevia, which includes S.eupatoria, S.purpurea and S .serrata (Lisitsin and kovalev, 2000).

  20. Molecular Characterization of Viruses from Clinical Respiratory Samples Producing Unidentified Cytopathic Effects in Cell Culture

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    Guy Boivin

    2009-07-01

    Full Text Available The sequence-independent single primer amplification (SISPA method was performed to identify a virus in 17 clinical respiratory samples producing uncharacterized cytopathic effects in LLC-MK2 cells. Sequence analysis of 600-1600 bp amplicons allowed the identification of six viruses (one influenza C, two parechovirus-3 and three cardioviruses. Genomic sequences of the cardioviruses showed similarities with those of the recently-described Saffold virus strain although significant variation was present in the viral surface EF and CD loops. These results demonstrate the usefulness of SISPA for identifying emerging viruses and also known viruses not easily identified by standard virological methods.

  1. To Introduce a Cultural Sense in Translation——A Brief Study of Non-equivalence in the Associated Meanings of English and Chinese Words

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This paper attempts to demonstrate the importance of intrpdicomg cultural sense in translation practice,English to Chinese in particular,by examining the problem of non-equivalence at word level in associated meanings and to unveil the cultural aspects projected within.It is also argued that greater importance should be attached to the cultural perspectives.in translation and flexible strategies should be adopted in dealing with the problems of non-equivalence in the associated meanings of words of different cultures.

  2. Culture of a high-chlorophyll-producing and halotolerant Chlorella vulgaris.

    Science.gov (United States)

    Nakanishi, Koichi; Deuchi, Keiji

    2014-05-01

    In order to increase the value of freshwater algae as raw ingredients for health foods and feed for seawater-based farmed fish, we sought to breed high-chlorophyll halotolerant Chlorella with the objective of generating strains with both high chlorophyll concentrations (≥ 5%) and halotolerance (up to 1% NaCl). We used the Chlorella vulgaris K strain in our research institute culture collection and induced mutations with UV irradiation and acriflavine which is known to effect mutations of mitochondrial DNA that are associated with chlorophyll production. Screenings were conducted on seawater-based "For Chlorella spp." (FC) agar medium, and dark-green-colored colonies were visually selected by macroscopic inspection. We obtained a high-chlorophyll halotolerant strain (designated C. vulgaris M-207A7) that had a chlorophyll concentration of 6.7% (d.m.), a level at least three-fold higher than that of K strain. This isolate also exhibited a greater survival rate in seawater that of K strain. Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  3. Screening and selection of high carotenoid producing in vitro tomato cell culture lines for [13C]-carotenoid production.

    Science.gov (United States)

    Engelmann, Nancy J; Campbell, Jessica K; Rogers, Randy B; Rupassara, S Indumathie; Garlick, Peter J; Lila, Mary Ann; Erdman, John W

    2010-09-22

    Isotopically labeled tomato carotenoids, phytoene, phytofluene, and lycopene, are needed for mammalian bioavailability and metabolism research but are currently commercially unavailable. The goals of this work were to establish and screen multiple in vitro tomato cell lines for carotenoid production, test the best producers with or without the bleaching herbicides, norflurazon and 2-(4-chlorophenyl-thio)triethylamine (CPTA), and to use the greatest carotenoid accumulator for in vitro 13C-labeling. Different Solanum lycopersicum allelic variants for high lycopene and varying herbicide treatments were compared for carotenoid accumulation in callus and suspension culture, and cell suspension cultures of the hp-1 line were chosen for isotopic labeling. When grown with [U]-13C-glucose and treated with CPTA, hp-1 suspensions yielded highly enriched 13C-lycopene with 45% of lycopene in the M+40 form and 88% in the M+35 to M+40 isotopomer range. To the authors' knowledge this is the first report of highly enriched 13C-carotenoid production from in vitro plant cell culture.

  4. Semantic Songket Image Search with Cultural Computing of Symbolic Meaning Extraction and Analytical Aggregation of Color and Shape Features

    Directory of Open Access Journals (Sweden)

    Desi Amirullah

    2015-06-01

    Full Text Available The term "Songket" comes from the Malay word "Sungkit", which means "to hook" or "to gouge". Every motifs names and variations was derived from plants and animals as source of inspiration to create many patterns of songket. Each of songket patterns have a philosophy in form of rhyme that refers to the nature of the sources of songket patterns and that philosophy reflects to the beliefs and values of Malay culture. In this research, we propose a system to facilitate an understanding of songket and the philosophy as a way to conserve Songket culture. We propose a system which is able to collect information in image songket motif variations based on feature extraction methods. On each image songket motif variations, we extracted philosophy of rhyme into impressions, and extracting color features of songket images using a histogram 3D-Color Vector quantization (3D-CVQ, shape feature extraction songket image using HU Moment invariants. Then, we created an image search based on impressions, and impressions search based on image. We use techniques of search based on color, shape and aggregation (combination of colors and shapes. The experiment using impression as query : 1 Result based on color, the average value of true 7.3, total score 41.9, 2 Result based on shape, the average value of true 3, total score 16.4, 3 Result based on aggregation, the average value of true 3, total score 17.4. While based using Image Query : 1 Result based on color, the average precision 95%, 2 Result based on shape, average precision 43.3%, 3 Based aggregation, the average precision 73.3%. From our experiments, it can be concluded that the best search system using query impression and query image is based on the color. Keyword : Image Search, Philosophy, impression, Songket, cultural computing, Feature Extraction, Analytical aggregation.

  5. Development and Use of a Real-Time Quantitative PCR Method for Detecting and Quantifying Equol-Producing Bacteria in Human Faecal Samples and Slurry Cultures

    Directory of Open Access Journals (Sweden)

    Lucía Vázquez

    2017-06-01

    Full Text Available This work introduces a novel real-time quantitative PCR (qPCR protocol for detecting and quantifying equol-producing bacteria. To this end, two sets of primers targeting the dihydrodaidzein reductase (ddr and tetrahydrodaidzein reductase (tdr genes, which are involved in the synthesis of equol, were designed. The primers showed high specificity and sensitivity when used to examine DNA from control bacteria, such as Slackia isoflavoniconvertens, Slackia equolifaciens, Asaccharobacter celatus, Adlercreutzia equolifaciens, and Enterorhabdus mucosicola. To demonstrate the validity and reliability of the protocol, it was used to detect and quantify equol-producing bacteria in human faecal samples and their derived slurry cultures. These samples were provided by 18 menopausal women under treatment of menopause symptoms with a soy isoflavone concentrate, among whom three were known to be equol-producers given the prior detection of the molecule in their urine. The tdr gene was detected in the faeces of all these equol-producing women at about 4–5 log10 copies per gram of faeces. In contrast, the ddr gene was only amplified in the faecal samples of two of these three women, suggesting the presence in the non-amplified sample of reductase genes unrelated to those known to be involved in equol formation and used for primer design in this study. When tdr and ddr were present in the same sample, similar copy numbers of the two genes were recorded. However, no significant increase in the copy number of equol-related genes along isoflavone treatment was observed. Surprisingly, positive amplification for both tdr and ddr genes was obtained in faecal samples and derived slurry cultures from two non-equol producing women, suggesting the genes could be non-functional or the daidzein metabolized to other compounds in samples from these two women. This novel qPCR tool provides a technique for monitoring gut microbes that produce equol in humans. Monitoring equol-producing

  6. A comparison of Culture-Free Self-Esteem Scale means from different child and adolescent groups.

    Science.gov (United States)

    Holaday, M; Callahan, K; Fabre, L; Hall, C; MacDonald, N; Mundy, M A; Owens, B; Plappert, H

    1996-06-01

    The Culture-Free Self-Esteem Inventory (CFSEI-2) was administered to 7 groups of children: 84 White Catholic school students from a New Orleans suburb, 78 White rural public school students from Virginia, 62 Hispanic Migrant student from Florida, 90 Aboriginal and White students from an isolated Canadian community, 199 African American students attending an inner city school, 60 Hispanic and White international students from Venezuela, and 61 Innuit students from isolated community in Labrador. The four elder groups also wrote three words to describe themselves (the Adjective Generation Technique [AGT]). Significant differences in responding between groups were found on all CFSEI-2 scales and for AGT favorability means. Although several possible reasons for these results are discussed, we conclude that the CFSEI-2 is not culture-free. Recommendations are: change the title of the test to avoid misrepresentation, limit test usage to elementary school children, develop an adolescent version with age appropriate language, and construct local norms before using the CFSEI-2 to make decisions about a child's self-esteem. To determine relevance of scores, a team of professionals and lay persons should review items from this or any test given to children who may be different from the normative or standardization group.

  7. Effect of different in vitro culture extracts of black pepper (Piper nigrum L.) on toxic metabolites-producing strains.

    Science.gov (United States)

    Ahmad, Nisar; Abbasi, Bilal Haider; Fazal, Hina

    2016-03-01

    In the present study, the effect of different in vitro cultures (callus, in vitro shoots) and commercially available peppercorn extract was investigated for its activity against toxic metabolite-producing strains (Escherichia coli, Pseudomonas aeroginosa, Salmonella typhi, Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, and Candida albicans). These in vitro cultures were extracted with ethanol, hexane, and chloroform, and the antipathogenic activity was determined by well-diffusion method. Hexane extract of callus showed 22 mm zone of inhibition against B. cereus, 23 mm against S. aureus, while regenerated shoots and seeds have shown 24.3 and 26 mm zones of inhibition. The ethanolic extracts of regenerated Piper shoots have shown 25 mm activity against S. aureus, 21 mm against B. cereus, and 16 mm in the case of C. albicans in comparison with standard antibiotics. Peppercorn extracts in chloroform and ethanol had shown activities against B. cereus (23.6 mm) and B. subtilis (23.5 mm). During in vitro organogenesis and morphogenesis, cells and tissues produced a comparable phytochemicals profile like mother plant. Morphogenesis is critically controlled by the application of exogenous plant-growth regulators. Such addition alters the hormonal transduction pathways, and cells under in vitro conditions regenerate tissues, which are dependant on the physiological state of cells, and finally enhance the production of secondary metabolites. To the best of our knowledge, this is the first report to compare the antimicrobial potential of in vitro regenerated tissues and peppercorn with standard antibiotics. In conclusion, most of the extracts showed pronounced activities against all the pathogenic microbes. This is a preliminary work, and the minimum inhibitory concentration values needs to be further explored. Regenerated tissues of P. nigrum are a good source of biologically active metabolites for antimicrobial activities, and callus culture presented itself as

  8. "This Guy Is Japanese Stuck in a White Man's Body": A Discussion of Meaning Making, Identity Slippage, and Cross-Cultural Adaption.

    Science.gov (United States)

    Armour, William S.

    2001-01-01

    Addresses two issues within a general theory of cross-cultural adaption. One concerns the extent to which cross-cultural adaption is activated by the ability to make meaning in Japanese as a foreign language; the second investigated the phenomenon of identity slippage. Six life histories of informants who had learned Japanese after age 11 are used…

  9. THE MEANINGS OF DWELLING ATTRIBUTES FOR TEMPORARY RESIDENTS FROM DIFFERENT CULTURES: THE CASE OF KOREAN TEMPORARY RESIDENTS IN THE UNITED STATES

    Directory of Open Access Journals (Sweden)

    Eunsil Lee

    2010-03-01

    Full Text Available The cross-cultural temporary resident population is one of the fastest growing cultural groups in the United States. However, their housing experiences in the new environment have not been extensively studied. Thus, the current study sought to examine meanings of dwelling attributes for cross-cultural temporary residents in the host country. In order to obtain insights into not only functional meanings but also underlying values, a conceptual framework was developed based primarily on Gutman’s (1982 means-end theory and Rapoport’s (1988 three levels of meaning. A case study was conducted using indepth laddering interviews with ten Korean temporary residents in the Lansing, Michigan, area. Seven dwelling attributes emerged from interviews: two satisfactory attributes (i.e., surrounding natural environment and architecture and five unsatisfactory attributes (i.e., carpeted floor, interior lighting, acoustics, bathroom, and entryway. Data were analyzed utilizing the measurement of means-end chain (Gutman, 1982, identifying the lower-level, everyday meanings as well as middle-level, latent meanings of dwelling attributes. A hierarchical value map was used to illustrate the interrelationships among the attributes, consequences, and values. Results revealed that dwelling attributes in participants’ current housing did not effectively satisfy their fundamental needs. In particular, carpeted floor was linked to the greatest number of negative meanings. Moreover, the cultural aspects of Korean housing affected the meanings of dwelling attributes in participants’ current homes. Findings suggest design professionals, facility managers, and policymakers must understand how people from other cultures attach different meanings to the dwelling attributes in their homes and provide more culturally responsive residential environments.

  10. Hematopoietic regulatory factors produced in long-term murine bone marrow cultures and the effect of in vitro irradiation

    International Nuclear Information System (INIS)

    Gualtieri, R.J.; Shadduck, R.K.; Baker, D.G.; Quesenberry, P.J.

    1984-01-01

    The nature of hematopoietic regulatory factors elaborated by the adherent (stromal) cells of long-term murine bone marrow cultures and the effect of in vitro stromal irradiation (XRT) on the production of these factors was investigated. Using an in situ stromal assay it was possible to demonstrate stromal elaboration of at least two colony-stimulating activities, ie, granulocyte/macrophage colony-stimulating activity (G/M-CSA) and megakaryocyte colony-stimulating activity (Meg-CSA). Exposure of the stroma to XRT resulted in dose-dependent elevations of both activities that correlated inversely with total myeloid cell mass. Mixture experiments that combined control and irradiated stroma revealed that the hematopoietically active control stroma could block detection of XRT-related G/M-CSA elevations. Antiserum directed against purified L cell colony-stimulating factor (CSF) reduced granulocyte/macrophage colony formation in the target layer but did not effect the increased Meg-CSA. While a radioimmunoassay for L-cell type CSF was unable to detect significant differences in concentrated media from control and irradiated cultures, bioassays of these media revealed XRT-related G/M-CSA elevations. These results indicate that the G/M-CSA elaborated in these cultures is immunologically distinct from the Meg-CSA produced, and although distinct from L cell CSF, the G/M-CSA is crossreactive with the L cell CSF antiserum. Morphologic, histochemical, and factor VII antigen immunofluorescent studies were performed on the stromal cell population responsible for production of these stimulatory activities. In addition to ''fat'' cells, the stromal cells remaining after XRT were composed of two predominant cell populations. These included a major population of acid phosphatase and nonspecific esterase-positive macrophage-like cells and a minor population of factor VII antigen negative epithelioid cells

  11. Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata.

    Science.gov (United States)

    Kakimoto, Masayuki; Ishikawa, Toshiki; Miyagi, Atsuko; Saito, Kazuaki; Miyazaki, Motonobu; Asaeda, Takashi; Yamaguchi, Masatoshi; Uchimiya, Hirofumi; Kawai-Yamada, Maki

    2014-02-15

    A volatile metabolite, 2-methylisoborneol (2-MIB), causes an unpleasant taste and odor in tap water. Some filamentous cyanobacteria produce 2-MIB via a two-step biosynthetic pathway: methylation of geranyl diphosphate (GPP) by methyl transferase (GPPMT), followed by the cyclization of methyl-GPP by monoterpene cyclase (MIBS). We isolated the genes encoding GPPMT and MIBS from Pseudanabaena galeata, a filamentous cyanobacterium known to be a major causal organism of 2-MIB production in Japanese lakes. The predicted amino acid sequence showed high similarity with that of Pseudanabaena limnetica (96% identity in GPPMT and 97% identity in MIBS). P. galeata was cultured at different temperatures to examine the effect of growth conditions on the production of 2-MIB and major metabolites. Gas chromatograph-mass spectrometry (GC-MS) measurements showed higher accumulation of 2-MIB at 30 °C than at 4 °C or 20 °C after 24 h of culture. Real-time-RT PCR analysis showed that the expression levels of the genes encoding GPPMT and MIBS decreased at 4 °C and increased at 30 °C, compared with at 20 °C. Furthermore, metabolite analysis showed dramatic changes in primary metabolite concentrations in cyanobacteria grown at different temperatures. The data indicate that changes in carbon flow in the TCA cycle affect 2-MIB biosynthesis at higher temperatures. Copyright © 2013 Elsevier GmbH. All rights reserved.

  12. Mean flow produced by small-amplitude vibrations of a liquid bridge with its free surface covered with an insoluble surfactant

    Science.gov (United States)

    Carrión, Luis M.; Herrada, Miguel A.; Montanero, José M.; Vega, José M.

    2017-09-01

    As is well known, confined fluid systems subject to forced vibrations produce mean flows, called in this context streaming flows. These mean flows promote an overall mass transport in the fluid that has consequences in the transport of passive scalars and surfactants, when these are present in a fluid interface. Such transport causes surfactant concentration inhomogeneities that are to be counterbalanced by Marangoni elasticity. Therefore, the interaction of streaming flows and Marangoni convection is expected to produce new flow structures that are different from those resulting when only one of these effects is present. The present paper focuses on this interaction using the liquid bridge geometry as a paradigmatic system for the analysis. Such analysis is based on an appropriate post-processing of the results obtained via direct numerical simulation of the system for moderately small viscosity, a condition consistent with typical experiments of vibrated millimetric liquid bridges. It is seen that the flow patterns show a nonmonotone behavior as the Marangoni number is increased. In addition, the strength of the mean flow at the free surface exhibits two well-defined regimes as the forcing amplitude increases. These regimes show fairly universal power-law behaviors.

  13. BRIDGING THE COMPETING VIEWS OF EUROPEAN CULTURAL INTEGRATION: THE TRANSFORMATIVE VIEW OF CULTURE AS A MEANS TO PROMOTE GROWTH, EMPLOYMENT AND SOCIAL COHESION

    Directory of Open Access Journals (Sweden)

    María Luz SUÁREZ CASTIÑEIRA

    2018-05-01

    Full Text Available The concept of a European culture became very complex with the enlargement of 2004 towards the East, when the EU, as Delanty pointed out, moved “beyond postnationality to an encounter with multiple civilizational forms,” multiple histories and competing visions of European integration. The “unity-in-diversity” paradigm turned into a huge challenge for the European institutions. On the one hand, achieving a European image of cultural unity without excluding all the local, regional and national cultures is a very complex, if not impossible, task. On the othe hand, culture remains an ambiguous term in European institutions due to the lack of a full-fledged European cultural policy. This paper focuses first on how in the early 1970s the EC/EU started to be concerned with defining the role of culture, and second on how since the year 2000 culture has progressively acquired a new status with potentially transformative powers to bridge the competing views of cultural integration. Programmes, such as the “2014-2020 Creative Europe” programme, focus on culture as a creative accelerator and promotor of different forms of cultural participation and production. Culture generates “smart, sustainable and inclusive growth”, and contributes to “high employment, high productivity, and high social cohesion.”

  14. An intensely sympathetic awareness: Experiential similarity and cultural norms as means for gaining older African Americans’ trust of scientific research

    Science.gov (United States)

    Sabir, Myra G.; Pillemer, Karl A.

    2014-01-01

    Well-known trust-building methods are routinely used to recruit and retain older African Americans into scientific research studies, yet the quandary over how to overcome this group’s hesitance to participate in research remains. We present two innovative and testable methods for resolving the dilemma around increasing older African Americans’ participation in scientific research studies. Certain specific and meaningful experiential similarities between the primary researcher and the participants, as well as clear recognition of the elders’ worth and dignity, improved older African Americans’ willingness to adhere to a rigorous research design. Steps taken in an intervention study produced a potentially replicable strategy for achieving strong results in recruitment, retention and engagement of this population over three waves of assessment. Sixty-two (n = 62) older African Americans were randomized to treatment and control conditions of a reminiscence intervention. Sensitivity to an African-American cultural form of respect for elders (recognition of worth and dignity), and intersections between the lived experience of the researcher and participants helped dispel this population’s well-documented distrust of scientific research. Results suggest that intentional efforts to honor the worth and dignity of elders through high level hospitality and highlighting meaningful experiential similarities between the researcher and the participants can improve recruitment and retention results. Experiential similarities, in particular, may prove more useful to recruitment and retention than structural similarities such as age, race, or gender, which may not in themselves result in the trust experiential similarities elicit. PMID:24655682

  15. Evolution of a recombinant (gucoamylase-producing) strain of Fusarium venenatum A3/5 in chemostat culture.

    Science.gov (United States)

    Wiebe, M G; Robson, G D; Shuster, J; Trinci, A P

    2001-04-20

    Fusarium venenatum JeRS 325 is a transformant of strain A3/5 which produces Aspergillus niger glucoamylase (GAM) under the control of a Fusarium oxysporum trypsin-like protease promoter. The evolution of JeRS 325 was studied in glucose-limited chemostat cultures grown on NaNO3 or (NH4)2SO4 as the nitrogen source. Thirteen mutants which were more highly branched and four mutants which were more sparsely branched than the parental strain were isolated from the NaNO3 chemostat. The highly branched mutants detected in this chemostat did not displace the sparsely branched population. The mutants isolated from the NaNO3 chemostat complemented representative strains previously isolated from glucose-limited chemostat cultures of F. venenatum A3/5 grown on (NH4)2SO4, but showed little complementation between themselves. By contrast, a highly branched mutant isolated from the (NH4)2SO4 chemostat culture displaced the sparsely branched mycelial population. None of the mutants isolated from the NaNO3 or (NH4)2SO4 chemostats produced as much GAM as JeRS 325. Southern blot analysis showed that all except one mutant had lost copies of both the glucoamylase and the acetamidase (the selectable marker) genes. However, specific GAM production was not necessarily correlated with the extent of glaA gene loss observed. Further, 10 of the mutants had lost the ability to grow on acetamide as the sole nitrogen source, although they retained copies of the amdS gene. In competition studies, mutants which could not utilize acetamide displaced mutants which could. The presence of foreign DNA in JeRS 325 resulted in a reduced specific growth rate (compared to A3/5), but the presence of the foreign DNA did not prevent the evolution of the strain or the isolation of mutants which had improved growth rates. Copyright 2001 John Wiley & Sons, Inc.

  16. Detection of hydrogen peroxide-producing Lactobacillus species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women

    Directory of Open Access Journals (Sweden)

    Balkus Jennifer E

    2012-08-01

    Full Text Available Abstract Background The presence of hydrogen peroxide (H2O2 producing Lactobacillus in the vagina may play a role in controlling genital HIV-1 shedding. Sensitive molecular methods improve our ability to characterize the vaginal microbiota; however, they cannot characterize phenotype. We assessed the concordance of H2O2-producing Lactobacillus detected by culture with quantitative PCR (qPCR detection of Lactobacillus species commonly assumed to be H2O2-producers. Methods Samples were collected as part of a prospective cohort study of HIV-1 seropositive US women. Cervicovaginal lavage specimens were tested for L. crispatus and L. jensenii using 16S rRNA gene qPCR assays. Vaginal swabs were cultured for Lactobacillus and tested for H2O2-production. We calculated a kappa statistic to assess concordance between culture and qPCR. Results Culture and qPCR results were available for 376 visits from 57 women. Lactobacilli were detected by culture at 308 (82% visits, of which 233 of 308 (76% produced H2O2. L. crispatus and/or L. jensenii were detected at 215 (57% visits. Concordance between detection of L. crispatus and/or L. jensenii by qPCR and H2O2-producing Lactobacillus by culture was 75% (kappa = 0.45. Conclusions Among HIV-1 seropositive women, there was a moderate level of concordance between H2O2-producing Lactobacillus detected by culture and the presence of L. crispatus and/or L. jensenii by qPCR. However, one-quarter of samples with growth of H2O2-producing lactobacilli did not have L. crispatus or L. jensenii detected by qPCR. This discordance may be due to the presence of other H2O2-producing Lactobacillus species.

  17. [Colors and their meaning in culture and psychology--a historical outline and contemporary status of color vision theories].

    Science.gov (United States)

    Grzybowski, Andrzej; Lewicka, Romana; Torlińska, Teresa; Stelcer, Bogusław

    2008-01-01

    The mechanism of color perception has intrigued scholars from antiquity. However, the understanding of this phenomena only came with the recognition of the nature of light and visual perception. Ancient concepts, present in science until the Renaissance, were based more on philosophical considerations and theoretical speculations than on anatomical studies and a matter-of-fact assessment of physiological functions of the visual system. From antiquity to 17th century scientific approach to the concept of vision was dominated by two theories: intromission and extramission (emanation). Intromission theory, propagated by Alhazen (lbn al.-Haythama), Vitello, John Peckham, Roger Bacon and Leonardo da Vinci, assumed that the light was transmitted from the observed object perpendicularly to the transparent eye structures. Johannes Kepler was the first scholar to propose that the retina was the receptive part of the eye. In the first half of the 17th century, Kepler's groundbreaking optical achievements and anatomical discoveries of many other scientists cast new light on the understanding of the role of different eye structures, finally wiping out the intromission theory. A further major achievement contributing to the recognition of the true nature of colors was a theory presented by Newton in 1688. He argued that they were colored rays, and not white light, that were composed of homogenous and pure light. It was, however, not until the 19th century when two modern theories of color appeared, i.e. a trichromatic theory mostly associated with the names of Young and Hemlholtz, and an opponent colors theory of Hering. In the 20th century, the two theories--previously assumed as contradictory--were joined into the zone theories of color vision. Colors have their cultural and social meanings, as far as a very individual and personal interpretation. In the former function they are used to illustrate some cultural and sociological phenomena; in the latter, they are helpful in

  18. Recombinant human IGF-1 produced by transgenic plant cell suspension culture enhances new bone formation in calvarial defects.

    Science.gov (United States)

    Poudel, Sher Bahadur; Bhattarai, Govinda; Kook, Sung-Ho; Shin, Yun-Ji; Kwon, Tae-Ho; Lee, Seung-Youp; Lee, Jeong-Chae

    2017-10-01

    Transgenic plant cell suspension culture systems have been utilized extensively as convenient and efficient expression systems for the production of recombinant human growth factors. We produced insulin-like growth factor-1 using a plant suspension culture system (p-IGF-1) and explored its effect on new bone formation in calvarial defects. We also compared the bone regenerating potential of p-IGF-1 with commercial IGF-1 derived from Escherichia coli (e-IGF-1). Male C57BL/6 mice underwent calvarial defect surgery, and the defects were loaded with absorbable collagen sponge (ACS) only (ACS group) or ACS impregnated with 13μg of p-IGF-1 (p-IGF-1 group) or e-IGF-1 (e-IGF-1 group). The sham group did not receive any treatment with ACS or IGFs after surgery. Live μCT and histological analyses showed critical-sized bone defects in the sham group, whereas greater bone formation was observed in the p-IGF-1 and e-IGF-1 groups than the ACS group both 5 and 10weeks after surgery. Bone mineral density, bone volume, and bone surface values were also higher in the IGF groups than in the ACS group. Local delivery of p-IGF-1 or e-IGF-1 more greatly enhanced the expression of osteoblast-specific markers, but inhibited osteoclast formation, in newly formed bone compared with ACS control group. Specifically, p-IGF-1 treatment induced higher expression of alkaline phosphatase, osteocalcin, and osteopontin in the defect site than did e-IGF-1. Furthermore, treatment with p-IGF-1, but not e-IGF-1, increased mineralization of MC3T3-E1 cells, with the attendant upregulation of osteogenic marker genes. Collectively, our findings suggest the potential of p-IGF-1 in promoting the processes required for bone regeneration. Copyright © 2017. Published by Elsevier Ltd.

  19. Strategies for efficiently selecting PHA producing mixed microbial cultures using complex feedstocks: Feast and famine regime and uncoupled carbon and nitrogen availabilities.

    Science.gov (United States)

    Oliveira, Catarina S S; Silva, Carlos E; Carvalho, Gilda; Reis, Maria A

    2017-07-25

    Production of polyhydroxyalkanoates (PHAs) by open mixed microbial cultures (MMCs) has been attracting increasing interest as an alternative technology to PHA production by pure cultures, due to the potential for lower costs associated with the use of open systems (eliminating the requirement for sterile conditions) and the utilisation of cheap feedstock (industrial and agricultural wastes). Such technology relies on the efficient selection of an MMC enriched in PHA-accumulating organisms. Fermented cheese whey, a protein-rich complex feedstock, has been used previously to produce PHA using the feast and famine regime for selection of PHA accumulating cultures. While this selection strategy was found efficient when operated at relatively low organic loading rate (OLR, 2g-CODL -1 d -1 ), great instability and low selection efficiency of PHA accumulating organisms were observed when higher OLR (ca. 6g-CODL -1 d -1 ) was applied. High organic loading is desirable as a means to enhance PHA productivity. In the present study, a new selection strategy was tested with the aim of improving selection for high OLR. It was based on uncoupling carbon and nitrogen supply and was implemented and compared with the conventional feast and famine strategy. For this, two selection reactors were fed with fermented cheese whey applying an OLR of ca. 8.5g-CODL -1 (with 3.8g-CODL -1 resulting from organic acids and ethanol), and operated in parallel under similar conditions, except for the timing of nitrogen supplementation. Whereas in the conventional strategy nitrogen and carbon substrates were added simultaneously at the beginning of the cycle, in the uncoupled substrates strategy, nitrogen addition was delayed to the end of the feast phase (i.e. after exogenous carbon was exhausted). The two different strategies selected different PHA-storing microbial communities, dominated by Corynebacterium and a Xantomonadaceae, respectively with the conventional and the new approaches. The new

  20. Bacterial community profiling of milk samples as a means to understand culture-negative bovine clinical mastitis.

    Science.gov (United States)

    Kuehn, Joanna S; Gorden, Patrick J; Munro, Daniel; Rong, Ruichen; Dong, Qunfeng; Plummer, Paul J; Wang, Chong; Phillips, Gregory J

    2013-01-01

    Inflammation and infection of bovine mammary glands, commonly known as mastitis, imposes significant losses each year in the dairy industry worldwide. While several different bacterial species have been identified as causative agents of mastitis, many clinical mastitis cases remain culture negative, even after enrichment for bacterial growth. To understand the basis for this increasingly common phenomenon, the composition of bacterial communities from milk samples was analyzed using culture independent pyrosequencing of amplicons of 16S ribosomal RNA genes (16S rDNA). Comparisons were made of the microbial community composition of culture negative milk samples from mastitic quarters with that of non-mastitic quarters from the same animals. Genomic DNA from culture-negative clinical and healthy quarter sample pairs was isolated, and amplicon libraries were prepared using indexed primers specific to the V1-V2 region of bacterial 16S rRNA genes and sequenced using the Roche 454 GS FLX with titanium chemistry. Evaluation of the taxonomic composition of these samples revealed significant differences in the microbiota in milk from mastitic and healthy quarters. Statistical analysis identified seven bacterial genera that may be mainly responsible for the observed microbial community differences between mastitic and healthy quarters. Collectively, these results provide evidence that cases of culture negative mastitis can be associated with bacterial species that may be present below culture detection thresholds used here. The application of culture-independent bacterial community profiling represents a powerful approach to understand long-standing questions in animal health and disease.

  1. Use of a solid-phase 3H-radioimmunoassay for the measurement of immunoglobulin produced in short-term cultures of antibody-secreting cells

    International Nuclear Information System (INIS)

    Mongini, P.K.A.; Heber-Katz, E.

    1982-01-01

    A solid-phase radioimmunoassay (RIA) for assaying immunoglobulin produced from antibody-secreting myeloma, hybridoma and immune spleen cells is described. Specific antibody is detected by culturing antibody-producing cells on antigen-coated flexible polyvinylchloride microtiter wells, washing away the cells, and measuring the bound specific antibody with tritiated affinity-purified anti-isotype reagents. Antibody produced from 10 3 myeloma cells can be detected with as little as 4 h of incubation. With 24-48 h of incubation, antibody from as few as approximately 3-15 myeloma, hybridoma or immune spleen plaque-forming cells (PFC) can be detected. This culture-well RIA has certain distinct advantages over the hemolytic PFC assay and RIA assays in which antibody in culture supernatants is measured. (Auth.)

  2. 3D+time acquisitions of 3D cell culture by means of lens-free tomographic microscopy

    Science.gov (United States)

    Berdeu, Anthony; Laperrousaz, Bastien; Bordy, Thomas; Morales, S.; Gidrol, Xavier; Picollet-D'hahan, Nathalie; Allier, Cédric

    2018-02-01

    We propose a three-dimensional (3D) imaging platform based on lens-free microscopy to perform multi-angle acquisitions on 3D cell cultures embedded in extracellular matrix (ECM). We developed algorithms based on the Fourier diffraction theorem to perform fully 3D reconstructions of biological samples and we adapted the lens-free microscope to incubator conditions. Here we demonstrate for the first time, 3D+time lens-free acquisitions of 3D cell culture over 8 days directly into the incubator. The 3D reconstructed volume is as large as 5 mm3 and provides a unique way to observe in the same 3D cell culture experiment multiple cell migration strategies. Namely, in a 3D cell culture of prostate epithelial cells embedded within a Matrigel® matrix, we are able to distinguish single cell 'leaders', migration of cell clusters, migration of large aggregates of cells, and also close-gap and large-scale branching. In addition, we observe long-scale 3D deformations of the ECM that modify the geometry of the 3D cell culture. Interestingly, we also observed the opposite, i.e. we found that large aggregates of cells may deform the ECM by generating traction forces over very long distances. In sum we put forward a novel 3D lens-free microscopy tomographic technique to study the single and collective cell migrations, the cell-to-cell interactions and the cell-to-matrix interactions.

  3. TV Dizileri Yoluyla Yeniden Üretilen Tüketim Kültürü / The Re-produced Consumption Culture by TV Series

    Directory of Open Access Journals (Sweden)

    Nesrin Kula

    2012-12-01

    Full Text Available “Tüketme” terimi “tahrip etmek, harcamak, israf etmek, bitirmek” anlamlarını taşımaktadır. Jean Baudrillard ise, tüketimi kodlar ve kurallarla düzenlenmiş bir göstergeler sistemi olarak tanımlamaktadır. Tüketim tarzları kişilerin kimliklerinin birer unsuru haline gelmektedir. Özneler kimliklerini ifade etmek amacıyla metaları kullanmakta ve kişilerin toplum içindeki statüsü tükettikleri ile ölçülür hale gelmektedir. TV dizileri de, bireylere tüketilecek sonsuz nesneler yoluyla oluşturulacak yaşam tarzları ile ilgili bilgiler vermektedir. Çalışmada, seçilen on dizide üretilen anlam dünyası, yeniden üretilen tüketim ideolojisi ve oluşturulan kadın ve erkek rol modelleri çözümlenmektir. The Re-produced Consumption Culture by TV Series The concept of consumption means ‘to destroy, spend, waste or finish something’. Jean Baudrillard describes the consumption as a semiotic system regulated of codes and rules. Consumption styles are nowadays becoming an element of people’s identity. Individuals use the commodities to express their identities and therefore the status of people in the community being measured according to what they consume. Meanwhile many TV series provides information to individuals about lifestyles to be created by infinite items consumed. This study analyzes the mindset created in ten selected TV series and the re-produced ideology of consumption as well as the presented role models for women and men in them.

  4. Culture-centered engagement with delivery of health services: co-constructing meanings of health in the Tzu Chi Foundation through Buddhist philosophy.

    Science.gov (United States)

    Dillard, Sydney J; Dutta, Mohan; Sun, Wei-San

    2014-01-01

    The shift in health communication scholarship from the narrow focus on curing to the complexly intertwined spaces of health, illness, healing, and curing attends to the dynamic cultural contexts within which meanings and practices are negotiated, directing scholarship toward alternative spaces of health care delivery. This study utilized the culture-centered approach as a theoretical lens for providing a discursive space for understanding meanings of health constituted in the practices of the Tzu Chi Foundation, an organization that offers biomedical services within the larger philosophical understandings of Buddhism with 10 million members in over 50 different countries. The emerging perspective promotes non-biomedical meanings of health through selfless giving and assistance founded in Buddhist principles, simultaneously seeking purity of the mind, body, and soul holistically. Through the negotiation of the principles driving Buddhist philosophy and the principles that shape biomedical health care delivery, this study seeks to understand the interpretive frames that circulate among foundation staff and care recipients.

  5. Comparison of the Multiple-sample means with composite sample results for fecal indicator bacteria by quantitative PCR and culture

    Science.gov (United States)

    ABSTRACT: Few studies have addressed the efficacy of composite sampling for measurement of indicator bacteria by QPCR. In this study, composite results were compared to single sample results for culture- and QPCR-based water quality monitoring. Composite results for both methods ...

  6. Meeting the Needs of Our Clients Creatively: The Impact of Art and Culture on Caregiving. Death, Value and Meaning Series.

    Science.gov (United States)

    Morgan, John D., Ed.

    This book integrates traditional understandings of care of the dying and bereaved with the use of arts and other forms of cultural creativity in therapy and funeralization. Authors provide insights into the practical aspects of caring for the dying and bereaved as well as new understandings of creativity. Chapters include: (1) "The Knowledge…

  7. The Britain that You don't Know-the historical and cultural meaning of British place names

    Institute of Scientific and Technical Information of China (English)

    翟瑜佳

    2014-01-01

    As products of social interactions between people, place namesalways represent parts of history and culture of the geographicalentities for they are created by using specific national languages.Therefore, we should look beyond the names themselves and dig forthe information behind them.

  8. The human and the inhuman: visual culture, political culture, and the images produced by George Rodger and Henri Cartier-Bresson in the Nazi concentration camps

    Directory of Open Access Journals (Sweden)

    Erika Cazzonatto Zerwes

    2016-08-01

    Full Text Available This article aims to grasp some aspects of the notion of humanism in photography and its closeness to the political culture and the visual culture in the period, through the specific experiences of George Rodger and Henri Cartier-Bresson, two photographers who were first-hand witnesses and provided accounts of horror in the Nazi concentration camps at the end of World War II. George Rodger photographed the Bergen-Belsen camp as soon as it was liberated by the British troops. Henri Cartier-Bresson was there with a film crew recording the deported masses newly freed from the Nazi concentration and extermination camps. These experiences came to have profound impact on the biography and work of both of them. In the two cases, there is a notion of humanism linked to World War II events, which is observed in photography and photographic representation, and it has a significant consequence for the contemporary visual culture.   Keywords: Visual Culture; Political Culture; War Photography; Photojournalism; Concentration Camps.   Original title: O humano e o desumano: cultura visual, cultura política e as imagens feitas por George Rodger e Henri Cartier-Bresson nos campos de concentração nazistas.

  9. Immobilization with Metal Hydroxides as a Means To Concentrate Food-Borne Bacteria for Detection by Cultural and Molecular Methods†

    OpenAIRE

    Lucore, Lisa A.; Cullison, Mark A.; Jaykus, Lee-Ann

    2000-01-01

    The application of nucleic acid amplification methods to the detection of food-borne pathogens could be facilitated by concentrating the organisms from the food matrix before detection. This study evaluated the utility of metal hydroxide immobilization for the concentration of bacterial cells from dairy foods prior to detection by cultural and molecular methods. Using reconstituted nonfat dry milk (NFDM) as a model, two food-borne pathogens (Listeria monocytogenes and Salmonella enterica sero...

  10. What Health and Aged Care Culture Change Models Mean for Residents and Their Families: A Systematic Review.

    Science.gov (United States)

    Petriwskyj, Andrea; Parker, Deborah; Brown Wilson, Christine; Gibson, Alexandra

    2016-04-01

    A range of commercialized programs are increasingly being adopted which involve broad culture change within care organizations to implement person-centered care. These claim a range of benefits for clients; however, the published evidence for client and family outcomes from culture change is inconclusive and the evidence for these specific models is difficult to identify. The purpose of this review was to identify and evaluate the peer-reviewed evidence regarding consumer outcomes for these subscription-based models. The review followed the Joanna Briggs Institute procedure. The review considered peer-reviewed literature that reported on studies conducted with health and aged care services, their staff, and consumers, addressed subscription-based person-centered culture change models, and were published in English up to and including 2015. The review identified 19 articles of sufficient quality that reported evidence relating to consumer outcomes and experience. Resident outcomes and family and resident satisfaction and experiences were mixed. Findings suggest potential benefits for some outcomes, particularly related to quality of life and psychiatric symptoms, staff engagement, and functional ability. Although residents and families identified some improvements in residents' lives, both also identified problematic aspects of the change related to staff adjustment and staff time. Outcomes for these models are at best comparable with traditional care with limited suggestions that they result in poorer outcomes and sufficient potential for benefits to warrant further investigation. Although these models may have the potential to benefit residents, the implementation of person-centered principles may affect the outcomes. © The Author 2015. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  11. Travel, emotion and identity: an exploration into the experiences of students in post 16 education for whom studying in English means working in a foreign language and culture

    OpenAIRE

    Underwood, M James

    2016-01-01

    This paper involves an exploration into the experiences of students in post 16 education taking traditional academic subjects, in the UK, for whom studying in English means working in a foreign language and culture. The students who formed the sample were high achieving students aged sixteen and seventeen from China, Vietnam and Thailand who had come to study in the UK in order to proceed to a British University. In the first part of this paper, the author proposes a conceptual framework suit...

  12. Terminal values and meaning in life among university students with varied levels of altruism in the present period of socio-cultural change

    Directory of Open Access Journals (Sweden)

    Głaz Stanisław

    2012-01-01

    Full Text Available Abstract The author of this paper, interested in the issues of values preference, meaning in life and altruism among university students has attempted to show a relation between them in the present period of clearly noticeable socio-cultural change. The study was conducted in 2009-2010 in Kraków among university students. The age of the respondents ranged from 21 to 25. 200 sets of correctly completed questionnaires were used for the results analysis.

  13. A grounded theory of football fan community identity and co-production: Consumer roles in brand culture, meaning, and value co-creation in virtual communities

    OpenAIRE

    Healy, Jason C.

    2012-01-01

    This study theorizes why and how consumers consume. Using a combined methodology, drawing upon Netnography and Grounded Theory, to study an online fan forum called RedAndWhiteKop, this thesis considers brand culture/meaning and value co-creation. The research site is a VC containing football fans who are views as stakeholders of the organisation Liverpool Football Club. Following emergent fit with woven in literature streams found in managerial marketing as Service-Dominant Logic and the con...

  14. Dignity and autonomy in the care for patients with dementia: Differences among formal caretakers of varied cultural backgrounds and their meaning.

    Science.gov (United States)

    Bentwich, Miriam Ethel; Dickman, Nomy; Oberman, Amitai

    A key message in the World Health Organization report on dementia (2012) emphasizes this disease as a top priority in public health and the need to improve professional attitudes to patients with dementia, while acknowledging that the workforce in dementia care is becoming increasingly diverse culturally. To trace whether there are substantial gaps between formal caretakers from different cultural groups (Israeli born Jews [Sabras], Israeli Arabs [Arabs] and migrants from Russia [Russians]) regarding their stances on the human dignity and autonomy of patients with dementia, as well as understand the meaning of these gaps. quantitative analysis utilizing questionnaires that were filled-out by approximately 200 caretakers from the different cultural groups, working in a nursing home or a hospital. In nursing homes, substantial differences were found in the attitudes to human dignity and autonomy of patients with dementia between Russian and Arab as well as Sabra caretakers. In the hospital, there was no influence for the ethno-culture variable on dignity or autonomy. Contrary to past research, in nursing homes, significant differences were found between certain ethno-cultural groups (Arabs and Russians) regarding their stance towards the dignity of patients with dementia. Arab caretakers hold a conception of dignity and autonomy that resonates strongly with person-centered care and outweighs institutional settings as well as may be related to the fostering of virtues. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  15. Analysis of the exopolysaccharides produced by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772 grown in continuous culture on glucose and fructose.

    NARCIS (Netherlands)

    Grobben, G.J.; Casteren, van W.H.M.; Schols, H.A.; Oosterveld, A.; Sala, G.; Smith, M.R.; Sikkema, J.; Bont, de J.A.M.

    1997-01-01

    The exopolysaccharides produced by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772 grown in defined medium were investigated. At equal cell densities, the strain produced 95 mg l−1 exopolysaccharides with glucose and 30 mg l−1 with fructose as the carbohydrate source. High-performance

  16. A Cross-Cultural Study on Meaning and the Nature of Children's Experiences in Australian and French Swimming Clubs

    Science.gov (United States)

    Light, Richard

    2010-01-01

    This article reports on a study conducted in Australia and France that inquired into the meaning and the nature of children's experiences of being in swimming clubs with a focus on the positive aspects of membership that keep them in their clubs. Three-month long case studies were conducted in a club in Australia and in a club in France, employing…

  17. Producing biodiesel from cotton seed oil using Rhizopus oryzae ATTC #34612 whole cell biocatalysts: Culture media and cultivation period optimization

    Science.gov (United States)

    The effect of culture medium composition and cultivation time on biodiesel production by Rhizopus oryzae ATCC #34612 whole cell catalysts, immobilized on novel rigid polyethylene biomass supports, was investigated. Supplementation of the medium with carbon sources led to higher lipase activity and i...

  18. Cellular changes in motor neuron cell culture produced by cytotoxic cerebrospinal fluid from patients with amyotrophic lateral sclerosis.

    Science.gov (United States)

    Gomez-Pinedo, U; Yáñez, M; Matías-Guiu, J; Galán, L; Guerrero-Sola, A; Benito-Martin, M S; Vela, A; Arranz-Tagarro, J A; García, A G

    2014-01-01

    The neurotoxic effects of cerebrospinal fluid (CSF) from patients with amyotrophic lateral sclerosis (ALS) have been reported by various authors who have attributed this neurotoxicity to the glutamate in CSF-ALS. Cultures of rat embryonic cortical neurons were exposed to CSF from ALS patients during an incubation period of 24 hours. Optical microscopy was used to compare cellular changes to those elicited by exposure to 100μm glutamate, and confocal microscopy was used to evaluate immunohistochemistry for caspase-3, TNFα, and peripherin. In the culture exposed to CSF-ALS, we observed cells with nuclear fragmentation and scarce or null structural modifications to the cytoplasmic organelles or to plasma membrane maintenance. This did not occur in the culture exposed to glutamate. The culture exposed to CSF-ALS also demonstrated increases in caspase-3, TNFα, and in peripherin co-locating with caspase-3, but not with TNFα, suggesting that TNFα may play an early role in the process of apoptosis. CFS-ALS cytotoxicity is not related to glutamate. It initially affects the nucleus without altering the cytoplasmic membrane. It causes cytoplasmic apoptosis that involves an increase in caspase-3 co-located with peripherin, which is also overexpressed. Copyright © 2013 Sociedad Española de Neurología. Published by Elsevier Espana. All rights reserved.

  19. EXPOSURE TO A P13KINASE INHIBITOR PRODUCED DYSMORPHOGENESIS IN NEURULATION-STAGED MOUSE EMBRYOS IN CULTURE

    Science.gov (United States)

    The haloacetic acids (HAA) are a family of chemicals that are drinking water disinfection byproducts. We previously reported that bromo- and chloro-acetic acids alter embryonic development when mouse conceptuses are directly exposed to these xenobiotics in whole embryo culture. C...

  20. Feasibility study of an alkaline-based chemical treatment for the purification of polyhydroxybutyrate produced by a mixed enriched culture

    NARCIS (Netherlands)

    Jiang, Y.; Mikova, G.; Kleerebezem, R.; van der Wielen, L.A.M.; Cuellar, M.C.

    2015-01-01

    This study focused on investigating the feasibility of purifying polyhydroxybutyrate (PHB) from mixed culture biomass by alkaline-based chemical treatment. The PHB-containing biomass was enriched on acetate under non-sterile conditions. Alkaline treatment (0.2 M NaOH) together with surfactant SDS

  1. Feasibility study of an alkaline-based chemical treatment for the purification of polyhydroxybutyrate produced by a mixed enriched culture

    NARCIS (Netherlands)

    Jiang, Y.; Mikova, G.; Kleerebezem, R.; Van der Wielen, L.A.M.; Cuellar Soares, M.C.

    2015-01-01

    This study focused on investigating the feasibility of purifying polyhydroxybutyrate (PHB) from mixed culture biomass by alkaline-based chemical treatment. The PHB-containing biomass was enriched on acetate under non-sterile conditions. Alkaline treatment (0.2 M NaOH) together with surfactant SDS

  2. Women in management means women in power: implications for society, family and culture in the Arab world.

    Science.gov (United States)

    King-irani, L

    1995-01-01

    A May 1995 conference in Beirut on "The Arab Woman and Business Management" focused on the fact that women are relatively absent from decision-making positions in the public and private sectors in the Arab world. Conference participants also identified characteristics of Arab women who have successfully penetrated the ranks of management, discussed how to balance work and home life, and considered the potentially important role of women managers in the nonprofit sector. Recurring and interrelated themes of the conference addressed the fact that women who have become empowered at work will have increased self-confidence in every aspect of their lives. Also, women who have successfully managed a busy household are able to transfer these skills to the workplace. Women will increasingly demand access to the highest ranks of decision-making, but their culturally-shaped sense of themselves may ultimately hinder them from attaining positions currently reserved for men. Research has shown that women's conceptions of power are more egalitarian and cooperative than the competitiveness exhibited by men. Women may, therefore, offer an important approach to the rapidly changing world market which requires the cooperation of people from diverse backgrounds. Ultimately, it is women who define themselves (rather than accept an identity chosen by society, the family, or culture) who will progress the fastest and achieve the highest ranks in management. Women should neither passively wait to be handed power not allow men to define the nature and uses of power. Instead they must seize their own power and exercise it in their own ways.

  3. Development of a chemically defined platform fed-batch culture media for monoclonal antibody-producing CHO cell lines with optimized choline content.

    Science.gov (United States)

    Kuwae, Shinobu; Miyakawa, Ichiko; Doi, Tomohiro

    2018-01-11

    A chemically defined platform basal medium and feed media were developed using a single Chinese hamster ovary (CHO) cell line that produces a monoclonal antibody (mAb). Cell line A, which showed a peak viable cell density of 5.9 × 10 6  cells/mL and a final mAb titer of 0.5 g/L in batch culture, was selected for the platform media development. Stoichiometrically balanced feed media were developed using glucose as an indicator of cell metabolism to determine the feed rates of all other nutrients. A fed-batch culture of cell line A using the platform fed-batch medium yielded a 6.4 g/L mAb titer, which was 12-fold higher than that of the batch culture. To examine the applicability of the platform basal medium and feed media, three other cell lines (A16, B, and C) that produce mAbs were cultured using the platform fed-batch medium, and they yielded mAb titers of 8.4, 3.3, and 6.2 g/L, respectively. The peak viable cell densities of the three cell lines ranged from 1.3 × 10 7 to 1.8 × 10 7  cells/mL. These results show that the nutritionally balanced fed-batch medium and feeds worked well for other cell lines. During the medium development, we found that choline limitation caused a lower cell viability, a lower mAb titer, a higher mAb aggregate content, and a higher mannose-5 content. The optimal choline chloride to glucose ratio for the CHO cell fed-batch culture was determined. Our platform basal medium and feed media will shorten the medium-development time for mAb-producing cell lines.

  4. Avoiding death or engaging life as accounts of meaning and culture: comment on Pyszczynski et al. (2004).

    Science.gov (United States)

    Ryan, Richard M; Deci, Edward L

    2004-05-01

    Terror management theory emphasizes that self-esteem consists of a sense of meaning and significance, which serves mainly to defend against death awareness. The current authors counter that people's search for meaning and significance cannot be wholly reduced to defensive processes because it also reflects intrinsic developmental processes. Sociometer theory similarly offers a mainly defensive account of self-esteem, and its exclusive focus on belongingness versus exclusion ill equips it to deal with the multiple needs underlying self-esteem. The current authors suggest that self-esteem resulting from defenses against anxiety (whether about death or exclusion) is akin to contingent self-esteem, whereas true self-esteem is based in ongoing satisfaction of needs for competence, autonomy, and relatedness. ((c) 2004 APA, all rights reserved)

  5. Distinct galactofuranose antigens in the cell wall and culture supernatants as a means to differentiate Fusarium from AspergillusspeciesAnnegret

    DEFF Research Database (Denmark)

    Wiedemann, Annegret; Kakoschke, Tamara Katharina; Speth, Cornelia

    2016-01-01

    tDetection of carbohydrate antigens is an important means for diagnosis of invasive fungal infections. Fordiagnosis of systemic Aspergillus infections, galactomannan is commonly used, the core antigenic struc-ture of which consists of chains of several galactofuranose moieties. In this study, we ....... fumigatus and Fusar-ium hyphae in immunohistology. Moreover, since Fusarium releases the AB135-8 antigen, it appears tobe a promising target antigen for a serological detection of Fusarium infections....

  6. Decolorization of the azo dye Acid Orange 51 by laccase produced in solid culture of a newly isolated Trametes trogii strain

    OpenAIRE

    Daâssi, Dalel; Zouari-Mechichi, Héla; Frikha, Fakher; Martínez, María Jesús; Nasri, M.; Mechichi, Tahar

    2013-01-01

    This study concerns the decolorization and detoxification of the azo dye Acid Orange 51 (AO51) by crude laccase from Trametes trogii produced in solid culture using sawdust as support media. A three-level Box?Behnken factorial design with four factors (enzyme concentration, 1-hydroxybenzotriazole (HBT) concentration, dye concentration and reaction time) combined with response surface methodology was applied to optimize AO51 decolorization. A mathematical model was developed showing the effect...

  7. Prebiotic potential of L-sorbose and xylitol in promoting the growth and metabolic activity of specific butyrate-producing bacteria in human fecal culture.

    Science.gov (United States)

    Sato, Tadashi; Kusuhara, Shiro; Yokoi, Wakae; Ito, Masahiko; Miyazaki, Kouji

    2017-01-01

    Dietary low-digestible carbohydrates (LDCs) affect gut microbial metabolism, including the production of short-chain fatty acids. The ability of various LDCs to promote butyrate production was evaluated in in vitro human fecal cultures. Fecal suspensions from five healthy males were anaerobically incubated with various LDCs. L-Sorbose and xylitol markedly promoted butyrate formation in cultures. Bacterial 16S rRNA gene-based denaturing gradient gel electrophoresis analyses of these fecal cultures revealed a marked increase in the abundance of bacteria closely related to the species Anaerostipes hadrus or A. caccae or both, during enhanced butyrate formation from L-sorbose or xylitol. By using an agar plate culture, two strains of A. hadrus that produced butyrate from each substrate were isolated from the feces of two donors. Furthermore, of 12 species of representative colonic butyrate producers, only A. hadrus and A. caccae demonstrated augmented butyrate production from L-sorbose or xylitol. These findings suggest that L-sorbose and xylitol cause prebiotic stimulation of the growth and metabolic activity of Anaerostipes spp. in the human colon. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  8. Cultural differences in the visual processing of meaning: detecting incongruities between background and foreground objects using the N400.

    Science.gov (United States)

    Goto, Sharon G; Ando, Yumi; Huang, Carol; Yee, Alicia; Lewis, Richard S

    2010-06-01

    East Asians have been found to allocate relatively greater attention to background objects, whereas European Americans have been found to allocate relatively greater attention to foreground objects. This is well documented across a variety of cognitive measures. We used a modification of the Ganis and Kutas (2003) N400 event-related potential design to measure the degree to which Asian Americans and European Americans responded to semantic incongruity between target objects and background scenes. As predicted, Asian Americans showed a greater negativity to incongruent trials than to congruent trials. In contrast, European Americans showed no difference in amplitude across the two conditions. Furthermore, smaller magnitude N400 incongruity effects were associated with higher independent self-construal scores. These data suggest that Asian Americans are processing the relationship between foreground and background objects to a greater degree than European Americans, which is consistent with hypothesized greater holistic processing among East Asians. Implications for using neural measures, the role of semantic processing to understand cultural differences in cognition, and the relationship between self construal and neural measures of cognition are discussed.

  9. The natural scorpion peptide, BmK NT1 activates voltage-gated sodium channels and produces neurotoxicity in primary cultured cerebellar granule cells.

    Science.gov (United States)

    Zou, Xiaohan; He, Yuwei; Qiao, Jinping; Zhang, Chunlei; Cao, Zhengyu

    2016-01-01

    The scorpion Buthus martensii Karsch has been used in Traditional Chinese Medicine to treat neuronal diseases such as neuropathic pain, paralysis and epilepsy for thousands of years. Studies have demonstrated that scorpion venom is the primary active component. Although scorpion venom can effectively attenuate pain in the clinic, it also produces neurotoxic response. In this study, toxicity guided purification led to identify a mammalian toxin termed BmK NT1 comprising of 65 amino acid residues and an amidated C-terminus, a mature peptide encoded by the nucleotide sequence (GenBank No. AF464898). In contract to the recombinant product of the same nucleotide sequence, BmK AGAP, which displayed analgesic and anti-tumor effect, intravenous injection (i.v.) of BmK NT1 produced acute toxicity in mice with an LD50 value of 1.36 mg/kg. In primary cultured cerebellar granule cells, BmK NT1 produced a concentration-dependent cell death with an IC50 value of 0.65 μM (0.41-1.03 μM, 95% Confidence Intervals, 95% CI) which was abolished by TTX, a voltage-gated sodium channel (VGSC) blocker. We also demonstrated that BmK NT1 produced modest sodium influx in cerebellar granule cell cultures with an EC50 value of 2.19 μM (0.76-6.40 μM, 95% CI), an effect similar to VGSC agonist, veratridine. The sodium influx response was abolished by TTX suggesting that BmK NT1-induced sodium influx is solely through activation of VGSC. Considered these data together, we demonstrated that BmK NT1 activated VGSC and produced neurotoxicity in cerebellar granule cell cultures. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. Particle formation induced by sonication during yogurt fermentation - Impact of exopolysaccharide-producing starter cultures on physical properties.

    Science.gov (United States)

    Körzendörfer, Adrian; Nöbel, Stefan; Hinrichs, Jörg

    2017-07-01

    Two major quality defects of yogurt are syneresis and the presence of large particles, and several reasons have been extensively discussed. Vibrations during fermentation, particularly generated by pumps, must be considered as a further cause as latest research showed that both ultrasound and low frequencies induced visible particles. The aim of this study was to investigate the impact of sonication during fermentation with starter cultures differing in exopolysaccharide (EPS) synthesis on the physical properties of set (syneresis, firmness) and stirred yogurt (large particles, laser diffraction, rheology). Skim milk was fermented with starter cultures YC-471 (low EPS) or YF-L 901 (high EPS) (Chr. Hansen) and sonicated for 5min at pH5.2. Sonicated set gels exhibited syneresis and were softer than respective controls. The mechanical treatment was adjusted to quantify visible particles (d≥0.9mm) in stirred yogurts properly. Sonication significantly increased particle numbers, however, the effect was less pronounced when YF-L 901 was used, indicating EPS as a tool to reduce syneresis and particle formation due to vibrations. Rheological parameters and size of microgel particles were rather influenced by starter cultures than by sonication. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Cultured human foreskin fibroblasts produce a factor that stimulates their growth with properties similar to basic fibroblast growth factor

    International Nuclear Information System (INIS)

    Story, M.T.

    1989-01-01

    To determine if fibroblasts could be a source of fibroblast growth factor (FGF) in tissue, cells were initiated in culture from newborn human foreskin. Fibroblast cell lysates promoted radiolabeled thymidine uptake by cultured quiescent fibroblasts. Seventy-nine percent of the growth-promoting activity of lysates was recovered from heparin-Sepharose. The heparin-binding growth factor reacted on immunoblots with antiserum to human placenta-derived basic FGF and competed with iodinated basic FGF for binding to antiserum to (1-24)bFGF synthetic peptide. To confirm that fibroblasts were the source of the growth factor, cell lysates were prepared from cells incubated with radiolabeled methionine. Heparin affinity purified material was immunoprecipitated with basic FGF antiserum and electrophoresed. Radiolabeled material was detected on gel autoradiographs in the same molecular weight region as authentic iodinated basic FGF. The findings are consistant with the notion that cultured fibroblasts express basic FGF. As these cells also respond to the mitogen, it is possible that the regulation of their growth is under autocrine control. Fibroblasts may be an important source of the growth factor in tissue

  12. Beneficial effect of two culture systems with small groups of embryos on the development and quality of in vitro-produced bovine embryos.

    Science.gov (United States)

    Cebrian-Serrano, A; Salvador, I; Silvestre, M A

    2014-02-01

    Currently, in vitro-produced embryos derived by ovum pick up (OPU) and in vitro fertilization (IVF) technologies represent approximately one-third of the embryos worldwide in cattle. Nevertheless, the culture of small groups of embryos from an individual egg donor is an issue that OPU-IVF laboratories have to face. In this work, we tested whether the development and quality of the preimplantation embryos in vitro cultured in low numbers (five embryos) could be improved by the addition of epidermal growth factor, insulin, transferrin and selenium (EGF-ITS) or by the WOW system. With this aim, immature oocytes recovered from slaughtered heifers were in vitro matured and in vitro fertilized. Presumptive zygotes were then randomly cultured in four culture conditions: one large group (LG) (50 embryos/500 μl medium) and three smaller groups [five embryos/50 μl medium without (control) or with EGF-ITS (EGF-ITS) and five embryos per microwell in the WOW system (WOW)]. Embryos cultured in LG showed a greater ability to develop to blastocyst stage than embryos cultured in smaller groups, while the blastocyst rate of WOW group was significantly higher than in control. The number of cells/blastocyst in LG was higher than control or WOW, whereas the apoptosis rate per blastocyst was lower. On the other hand, the addition of EGF-ITS significantly improved both parameters compared to the control and resulted in similar embryo quality to LG. In conclusion, the WOW system improved embryo development, while the addition of EGF-ITS improved the embryo quality when smaller groups of embryos were cultured. © 2013 Blackwell Verlag GmbH.

  13. Alternatives for biosurfactants and bacteriocins extraction from Lactococcus lactis cultures produced under different pH conditions.

    Science.gov (United States)

    Rodríguez, N; Salgado, J M; Cortés, S; Domínguez, J M

    2010-08-01

    Study of the potential of Lactococcus lactis CECT-4434 as a biosurfactants and nisin (the only bacteriocin allowed to be used in the food industry) producer for industrial applications, exploiting the possibility of recovering separately both metabolites, taking into account that L. lactis is an interesting micro-organism with several applications in the food industry because it is recognized as GRAS. The results showed the ability of this strain to produce cell-bound biosurfactants, under controlled pH, and cell-bound biosurfactants and bacteriocins, when pH was not controlled. Three extraction procedures were designed to separately recover these substances. The strain L. lactis CECT-4434 showed to be a cell-bound biosurfactants and bacterocins producer when fermentations were carried out under uncontrolled pH. Both products can be recovered separately. Development of a convenient tool for the extraction of cell-bound biosurfactants and bacteriocins from the fermentation broth.

  14. Ethno-cultural variations in the experience and meaning of mental illness and treatment: implications for access and utilization.

    Science.gov (United States)

    Carpenter-Song, Elizabeth; Chu, Edward; Drake, Robert E; Ritsema, Mieka; Smith, Beverly; Alverson, Hoyt

    2010-04-01

    We conducted a study to investigate how understandings of mental illness and responses to mental health services vary along ethno-racial lines. Participants were 25 African American, Latino, and Euro-American inner-city residents in Hartford Connecticut diagnosed with severe mental illness and currently enrolled in a larger study of a community mental health center. Data were collected through 18 months of ethnographic work in the community. Overall, Euro-Americans participants were most aligned with professional disease-oriented perspectives on severe mental illness and sought the advice and counsel of mental health professionals. African-American and Latino participants emphasized non-biomedical interpretations of behavioral, emotional, and cognitive problems and were critical of mental health services. Participants across the sample expressed expectations and experiences of psychiatric stigma. Although Euro-Americans were aware of the risk of social rejection because of mental illness, psychiatric stigma did not form a core focus of their narrative accounts. By contrast, stigma was a prominent theme in the narrative accounts of African Americans, for whom severe mental illness was considered to constitute private "family business." For Latino participants, the cultural category of nervios appeared to hold little stigma, whereas psychiatric clinical labels were potentially very socially damaging. Our findings provide further empirical support for differences in symptom interpretation and definitions of illness among persons from diverse ethno-racial backgrounds. First-person perspectives on contemporary mental health discourses and practices hold implications for differential acceptability of mental health care that may inform variations in access and utilization of services in diverse populations.

  15. Embodied Cultures of Mobilities

    DEFF Research Database (Denmark)

    Jensen, Ole B.

    2010-01-01

    and material artifacts. The paper target the complex relationship between the moving, sensing body and the material and built environment of infrastructures and mobility modes in order to explore what norms, and meanings, and everyday life mobility cultures are being produced and re-produced in this process....... A particular emphasis is put on how mobilities produce and re-produce norms, meanings and cultures in relation to the Everyday life perspective. By looking into walking, running, cycling, driving and mass transit mobilities different modes of embodied mobility is identified. The theoretical framework is based...... and interaction (Lynch). The argument is thus that understanding embodied cultures of mobilities from the vantage point of this paper lend is self to new interpretations, explorations and understandings of what it means to move within and between other social agents in particular material and physical...

  16. Soft X-ray generation in gases by means of a pulsed electron beam produced in a high-voltage barier discharge

    NARCIS (Netherlands)

    Azarov, A.V.; Peters, P.J.M.; Boller, Klaus J.

    2007-01-01

    A large area pulsed electron beam is produced by a high-voltage barrier discharge. We compare the properties of the x-rays generated by stopping this beam of electrons in a thin metal foil with those generated by stopping the electrons directly in various gases. The generation of x-rays was

  17. Producing a particle-reinforced AlCuMgMn alloy by means of mechanical alloying; Herstellung einer partikelverstaerkten AlCuMgMn-Legierung durch mechanisches Legieren

    Energy Technology Data Exchange (ETDEWEB)

    Nestler, D.; Wielage, B. [TU Chemnitz, Institut fuer Werkstoffwissenschaft und Werkstofftechnik (Germany); Siebeck, S.

    2012-07-15

    High-energy ball milling (HEM) with subsequent consolidation is a suitable method to produce particle-reinforced aluminium materials. The task of HEM is to distribute the reinforcement particles as homogeneously as possible. A further application of HEM is mechanical alloying (MA). This paper deals with the combination of both applications. Pure metallic powders (Al, Cu, Mg, Mn) were milled together with SiC particles up to 10 h. The composition of the metallic powder corresponds to that of the alloy AA2017 (3.9% Cu, 0.7% Mg, 0.6% Mn). In previous experiments [1], this alloy was used in the form of atomized powder. The changes in microstructure during the formation of the composite powder have been studied by light microscopy, SEM, EDXS and XRD. The results show that the production of composite powders in a single step is possible. This not only allows the economical production of such powders, but also facilitates the use of alloy compositions that are not producible via the melting route, or only producible with difficulty via the melting route. It's possible to produce tailor-made-alloys. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  18. Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002.

    Science.gov (United States)

    Therien, Jesse B; Zadvornyy, Oleg A; Posewitz, Matthew C; Bryant, Donald A; Peters, John W

    2014-01-01

    The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. Here we demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC 7002. Optimal growth conditions for co-cultivation of C. reinhardtii with wild-type and mutant strains of Synechococcus sp. 7002 were established. In co-culture, acetate produced by a glycogen synthase knockout mutant of Synechococcus sp. PCC 7002 was able to support the growth of a lipid-accumulating mutant strain of C. reinhardtii defective in starch production. Encapsulation of Synechococcus sp. PCC 7002 using an alginate matrix was successfully employed in co-cultures to limit growth and maintain the stability. The ability of immobilized strains of the cyanobacterium Synechococcus sp. PCC 7002 to produce acetate at a level adequate to support the growth of lipid-accumulating strains of C. reinhartdii offers a potentially practical, photosynthetic alternative to providing exogenous acetate into growth media.

  19. Unusual Δ7,12,19 C35:3 Alkenone Produced by the Mutant Emiliania huxleyi strain CCMP2758 in Culture

    Science.gov (United States)

    Zheng, Y.; Huang, Y.; Zhang, Y.; Dillon, J. T.

    2015-12-01

    Alkenones with chain length ranging from C37 to C40 are highly specific biomarkers for certain haptophyte algae in ocean and lake sediments and have been widely used for paleoclimate studies. Short chain alkenones (e.g., C35 and C36) have been found in environmental and culture samples but the origin and structures of these compounds are not fully understood. The benchmark marine alkenone producer, Emiliania huxleyi CCMP2758 strain (the mutant of strain CCMP1742, NEPCC55a) was reported to make 35:2 alkenone when cultured at 15 °C (Prahl et al., 2006). Here we show, when this strain is cultured at lower temperatures (e.g., 4°C), CCMP2758 produces large amount of 35:3 alkenone with unusual double bond positions of Δ7,12,19. We determined the double bond positions of the C35:3 methyl ketonee based on GC-MS analysis of cyclobutylimine derivatives and dimethyl disulfide derivatives respectively, and provide the first temperature calibrations based on the unsaturation ratios of C35 alkenones. Previous studies have found 35:2 alkenone with three methylene interruption in the Black Sea sediment, but it is the first time that an alkenone with a mixed three and five methylene interruption is found. The discovery of short chain alkenones with unusual double bond positions may shed new light to alkenone biosynthesis.

  20. cultural

    Directory of Open Access Journals (Sweden)

    Irene Kreutz

    2006-01-01

    Full Text Available Es un estudio cualitativo que adoptó como referencial teorico-motodológico la antropología y la etnografía. Presenta las experiencias vivenciadas por mujeres de una comunidad en el proceso salud-enfermedad, con el objetivo de comprender los determinantes sócio-culturales e históricos de las prácticas de prevención y tratamiento adoptados por el grupo cultural por medio de la entrevista semi-estructurada. Los temas que emergieron fueron: la relación entre la alimentación y lo proceso salud-enfermedad, las relaciones con el sistema de salud oficial y el proceso salud-enfermedad y lo sobrenatural. Los dados revelaron que los moradores de la comunidad investigada tienen un modo particular de explicar sus procedimientos terapéuticos. Consideramos que es papel de los profesionales de la salud en sus prácticas, la adopción de abordajes o enfoques que consideren al individuo en su dimensión sócio-cultural e histórica, considerando la enorme diversidad cultural en nuestro país.

  1. Screening and Selection of High Carotenoid Producing in Vitro Tomato Cell Culture Lines for [13C]-Carotenoid Production

    OpenAIRE

    Engelmann, Nancy J.; Campbell, Jessica K.; Rogers, Randy B.; Rupassara, S. Indumathie; Garlick, Peter J.; Lila, Mary Ann; Erdman, John W.

    2010-01-01

    Isotopically labeled tomato carotenoids, phytoene, phytofluene, and lycopene, are needed for mammalian bioavailability and metabolism research but are currently commercially unavailable. The goals of this work were to establish and screen multiple in vitro tomato cell lines for carotenoid production, test the best producers with or without the bleaching herbicides, norflurazon and 2-(4-chlorophenyl-thio)-triethylamine (CPTA), and to use the greatest carotenoid accumulator for in vitro 13C-lab...

  2. Evaluation of the Synergistic Effect of Mixed Cultures of White-Rot Fungus Pleurotus ostreatus and Biosurfactant-Producing Bacteria on DDT Biodegradation.

    Science.gov (United States)

    Purnomo, Adi Setyo; Ashari, Khoirul; Hermansyah, Farizha Triyogi

    2017-07-28

    DDT (1,1,1-trichloro-2,2- bis (4-chlorophenyl) ethane) is one of the organic synthetic pesticides that has many negative effects for human health and the environment. The purpose of this study was to investigate the synergistic effect of mixed cutures of white-rot fungus, Pleurotus ostreatus , and biosurfactant-producing bacteria, Pseudomonas aeruginosa and Bacillus subtilis , on DDT biodegradation. Bacteria were added into the P. ostreatus culture (mycelial wet weight on average by 8.53 g) in concentrations of 1, 3, 5, and 10 ml (1 ml ≈ 1.25 × 10 9 bacteria cells/ml culture). DDT was degraded to approximately 19% by P. ostreatus during the 7-day incubation period. The principal result of this study was that the addition of 3 ml of P. aeruginosa into P. ostreatus culture gave the highest DDT degradation rate (approximately 86%) during the 7-day incubation period. This mixed culture combination of the fungus and bacteria also gave the best ratio of optimization of 1.91. DDD (1,1-dichloro-2,2- bis (4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2- bis (4-chlorophenyl) ethylene), and DDMU (1-chloro-2,2- bis (4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by P. ostreatus and P. aeruginosa . The results of this study indicate that P. aeruginosa has a synergistic relationship with P. ostreatus and can be used to optimize the degradation of DDT by P. ostreatus .

  3. Apoptosis induced by pyrimidine dimer produced by ultraviolet irradiation in cultured cyprinodont cells. Analysis utilizing the evasion from photolysis

    International Nuclear Information System (INIS)

    Nishigaki, Reiko

    2000-01-01

    This is the review of author's investigations on the mechanism of apoptosis induced by UV irradiation in cultured cyprinodont cells highly expressing photolyases of cyclobutane pyrimidine dimer (CPD). Authors found out the evasion from photolysis by UV-induced apoptosis of which cascade had been thought irreversible: the cascade was reversible until the process of DNA fragmentation. In controlling the process, a mechanism to recognize CPD was found concerned. In those cells, morphological changes by UVC were reversible ones in apoptosis from which they evaded if CPD was repaired. Investigations on caspase, which playing important roles in apoptosis, revealed that the DEVD-cleaving enzyme activities were regulated by CPD quantity. However, differing from mammalian cells, elevation of caspase (-7, -3A and -3B) activities did not always induce the morphologic changes and DNA fragmentation. Further studies were thought necessary to elucidate the mechanism of apoptosis in those fish cells. (K.H.)

  4. Not saying I am happy does not mean I am not: cultural influences on responses to positive affect items in the CES-D.

    Science.gov (United States)

    Jang, Yuri; Kwag, Kyung Hwa; Chiriboga, David A

    2010-11-01

    Given the emphasis on modesty and self-effacement in Asian societies, the present study explored differential item responses for 2 positive affect items (5 = Hopeful and 8 = Happy) on a short form of the Center for Epidemiologic Studies-Depression scale. The samples consisted of elderly non-Hispanic Whites (n = 450), Korean Americans (n = 519), and Koreans (n = 2,030). Multiple Indicator Multiple Cause models were estimated to identify the impact of group membership on responses to the positive affect items while controlling for the latent trait of depressive symptoms. The data revealed that Koreans and Korean Americans were less likely than non-Hispanic Whites to endorse the positive affect items. Compared with Korean Americans who were more acculturated to mainstream American culture, those who were less acculturated were less likely to endorse the positive affect items. Our findings support the notion that the way in which people endorse depressive symptoms is substantially influenced by cultural orientation. These findings call into question the common use of simple mean comparisons and a universal cutoff point across diverse cultural groups.

  5. Culture, economics, politics and knowledge as meaning-spaces in Social Occupational Therapy: reflections on the experience of “Ponto de Encontro e Cultura”

    Directory of Open Access Journals (Sweden)

    Denise Dias Barros

    2013-12-01

    Full Text Available This article describes and analyzes the “Ponto de Encontro e Cultura - PEC”, an experience developed by METUIA - University of São Paulo (USP-SP in São Paulo from 2007 to 2011 which linked occupational therapy to areas of cultural production. It was attended by the homeless, occupational therapists and students of occupational therapy. To perform the analysis of the different dimensions of the experience we were guided by the meaning-space notion. We took the space as an organizer to understand a reality in which relationships and actions are being empowered in four different spheres: culture, economics, politics and knowledge. We noted that this practice showed that there was an ongoing collective effort to build what may be called piece. This characterizes a process where the space is a common reference point which brings into play different modes of sociability that are created by the management of common symbols and codes. It was important to recognize and appreciate the plurality of modes of knowledge. Thus, we observed that, from the cultural sphere, it is possible to articulate economics, health, social assistance, politics, and knowledge production.

  6. Use of the α-mannosidase I inhibitor kifunensine allows the crystallization of apo CTLA-4 homodimer produced in long-term cultures of Chinese hamster ovary cells

    International Nuclear Information System (INIS)

    Yu, Chao; Crispin, Max; Sonnen, Andreas F.-P.; Harvey, David J.; Chang, Veronica T.; Evans, Edward J.; Scanlan, Christopher N.; Stuart, David I.; Gilbert, Robert J. C.; Davis, Simon J.

    2011-01-01

    The α-mannosidase I inhibitor kifunensine inhibited N-glycan processing in long-term cultures of Chinese hamster ovary cells, allowing deglycosylation and crystallization of the homodimeric extracellular region of the inhibitory glycoprotein receptor CTLA-4 (CD152). Glycoproteins present problems for structural analysis since they often have to be glycosylated in order to fold correctly and because their chemical and conformational heterogeneity generally inhibits crystallization. It is shown that the α-mannosidase I inhibitor kifunensine, which has previously been used for the purpose of glycoprotein crystallization in short-term (3–5 d) cultures, is apparently stable enough to be used to produce highly endoglycosidase H-sensitive glycoprotein in long-term (3–4 week) cultures of stably transfected Chinese hamster ovary (CHO) cells. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based analysis of the extracellular region of the cytotoxic T-lymphocyte antigen 4 (CTLA-4; CD152) homodimer expressed in long-term CHO cell cultures in the presence of kifunensine revealed that the inhibitor restricted CTLA-4 glycan processing to Man 9 GlcNAc 2 and Man 5 GlcNAc 2 structures. Complex-type glycans were undetectable, suggesting that the inhibitor was active for the entire duration of the cultures. Endoglycosidase treatment of the homodimer yielded protein that readily formed orthorhombic crystals with unit-cell parameters a = 43.9, b = 51.5, c = 102.9 Å and space group P2 1 2 1 2 1 that diffracted to Bragg spacings of 1.8 Å. The results indicate that kifunensine will be effective in most, if not all, transient and long-term mammalian cell-based expression systems

  7. Producing access for the elderly to territories of culture: an experience of occupational therapy in an art museum

    Directory of Open Access Journals (Sweden)

    Ana Tereza Costa Galvanese

    2014-04-01

    Full Text Available From 1996 to 2009, the Laboratory for Studies and Research in Art, Body and Occupational Therapy established a cooperation with the Museum of Contemporary Art of USP (MAC USP, working in partnership with the Leisure and Art to the Elderly Program of the Education and Technical-Scientific Division of MAC USP. The program offers an introduction in contemporary artistic practice to the elderly. This paper presents the interdisciplinary experience developed in this partnership in 2006. The method adopted in the program is referenced in the Triangular Approach to Teaching Art. Therefore, the appreciation of works of art and the contextualization of selected artists formed the basis on which participants developed their own poetics. The preparatory work was developed in group dynamics, including activities of body awareness and conversation circles coordinated by occupational therapists and students. They also accompanied the participants in their demands related to the challenges of constructing access to socio-cultural territories. The relevance of this living process was evident in the topics proposed by participants in conversations, or arisen during the body work. The aesthetic quality of the participants’ production resulted in personal and collective satisfaction and provoked admiration of the public who visited the workshop and exhibition, organized from this production.

  8. Production of sensory compounds by means of the yeast Dekkera bruxellensis in different nitrogen sources with the prospect of producing cachaça.

    Science.gov (United States)

    Castro Parente, Denise; Vidal, Esteban Espinosa; Leite, Fernanda Cristina Bezerra; de Barros Pita, Will; de Morais, Marcos Antonio

    2015-01-01

    The distilled spirit made from sugar cane juice, also known as cachaça, is a traditional Brazilian beverage that in recent years has increased its market share among international distilled beverages. Several volatile compounds produced by yeast cells during the fermentation process are responsible for the unique taste and aroma of this drink. The yeast Dekkera bruxellensis has acquired increasing importance in the fermented beverage production, as the different metabolites produced by this yeast may be either beneficial or harmful to the end-product. Since D. bruxellensis is often found in the fermentation processes carried out in ethanol fuel distillation in Brazil, we employed this yeast to analyse the physiological profile and production of aromatic compounds and to examine whether it is feasible to regard it as a cachaça-producing microorganism. The assays were performed on a small scale and simulated the conditions for the production of handmade cachaça. The results showed that the presence of aromatic and branched-chain amino acids in the medium has a strong influence on the metabolism and production of flavours by D. bruxellensis. The assimilation of these alternative nitrogen sources led to different fermentation yields and the production of flavouring compounds. The influence of the nitrogen source on the metabolism of fusel alcohols and esters in D. bruxellensis highlights the need for further studies of the nitrogen requirements to obtain the desired level of sensory compounds in the fermentation. Our results suggest that D. bruxellensis has the potential to play a role in the production of cachaça. Copyright © 2014 John Wiley & Sons, Ltd.

  9. Preliminary viability studies of fibroblastic cells cultured on microcrystalline and nanocrystalline diamonds produced by chemical vapour deposition method

    Directory of Open Access Journals (Sweden)

    Ana Amélia Rodrigues

    2013-02-01

    Full Text Available Implant materials used in orthopedics surgery have demonstrated some disadvantages, such as metallic corrosion processes, generation of wear particles, inflammation reactions and bone reabsorption in the implant region. The diamond produced through hot-filament chemical vapour deposition method is a new potential biomedical material due to its chemical inertness, extreme hardness and low coefficient of friction. In the present study we analysis two samples: the microcrystalline diamond and the nanocrystalline diamond. The aim of this study was to evaluate the surface properties of the diamond samples by scanning electron microscopy, Raman spectroscopy and atomic force microscopy. Cell viability and morphology were assessed using thiazolyl blue tetrazolium bromide, cytochemical assay and scanning electron microscopy, respectively. The results revealed that the two samples did not interfere in the cell viability, however the proliferation of fibroblasts cells observed was comparatively higher with the nanocrystalline diamond.

  10. The application of biotechnology to the treatment of wastes produced from the nuclear fuel cycle: Biodegradation and bioaccumulation as a means of treating radionuclide-containing streams

    International Nuclear Information System (INIS)

    Macaskie, L.E.

    1991-01-01

    Recent concerns on the radiotoxicity and longevity of nuclides have prompted the development of new technologies for their removal from wastes produced from nuclear power programs and nuclear fuel reprocessing activities. Alongside developments from traditional chemical treatment processes, interest has also centered on the application of biotechnology for efficient waste treatment. Many biological techniques have relied on empirical approaches in simple model systems, with scant regard to the nature and volume of actual target wastes; such considerations may limit the application of the new technologies in practice. This review aims to identify some of the likely problems, to discuss the various approaches under current consideration, and to evaluate ways in which either the target waste or the detoxifying biomass may be modified or presented for the most efficient treatment. 278 references

  11. Effects of muffin processing on fumonisins from 14C-labeled toxins produced in cultured corn kernels.

    Science.gov (United States)

    Avantaggiato, Giuseppina; De La Campa, Regina; Miller, J David; Visconti, Angelo

    2003-10-01

    The persistence of fumonisins during cooking is known to be affected by several factors, including thermal degradation and the presence of various ingredients in corn-based food recipes that can react with the toxin. A method for the production of corn kernels containing 14C-fumonisins was developed. The corn kernels were colonized by Fusarium verticillioides MRC 826 and supplemented with 1,2-14C-sodium acetate. The specific activity of 14C-FB1 produced made the study of its fate in cornmeal muffins possible. The double-extraction acetonitrile-water-methanol/immunoaffinity column/o-phthaldialdehyde high-performance liquid chromatography (HPLC) method was used to determine FB1 levels in cornmeal muffins. Reductions in FB1 levels in muffins spiked with 14C-labeled and unlabeled FB1 (43 and 48%, respectively) were similar, indicating that the extraction method was efficient and consistent with previous reports. However, with the labeled corn kernel material, recovery levels based on the 14C counts for the eluate from an immunoaffinity column were much higher (90%). This finding indicates that some fumonisin-related compounds other than FB1 that were present in the cornmeal were recognized by the antibodies but not by the HPLC method.

  12. Stress- and sequence-dependent release into the culture medium of HIV-1 Nef produced in Saccharomyces cerevisiae.

    Science.gov (United States)

    Macreadie, I G; Castelli, L A; Lucantoni, A; Azad, A A

    1995-09-11

    We have produced human immunodeficiency virus type 1 (HIV-1) Nef (a myristylated 206-amino-acid protein) in Saccharomyces cerevisaie and shown that, while Nef is normally found as a predominantly intracellular protein, amounts up to 40 micrograms/ml of Nef are also released into the extracellular medium during stress. By electrophoretic (SDS-PAGE) analysis the extracellular Nef is indistinguishable from intracellular Nef. Conditions of stress that lead to the release of Nef include elevated levels of copper or magnesium ions or growth at elevated temperatures. This release appears to be dependent upon the N-terminal sequences of Nef, including the presence of a myristylation site. Our observations concerning Nef release in yeast suggest new ways in which the behaviour of Nef should be examined in order to gain further insights into the development of AIDS. If the release of Nef is important in the development of AIDS, our work reveals that Nef-associated symptoms may be reduced or delayed by reducing stresses, such as fevers.

  13. Application of mutation breeding technique for producing NaCl tolerant plants of banana in tissue culture and greenhouse conditions

    International Nuclear Information System (INIS)

    Vedadi, C.; Rahimi, M.; Naserian, B.; Rahmani, E.; Neshan, N.

    2005-01-01

    Full text: To study of possibility to induce salt tolerant clones in banana by using mutation technique, an experiment was conducted with factorial (gamma irradiation and salt concentration factors) in a CRD design. In this research, plantlets of banana cv. Dwarf Cavendish were produced by subculture of irradiated shoot tips. It deserves to mention that consequent subculturing was aimed at getting rid of chimera. Next, these explants were transferred to MS medium containing 2.5 mg. l- 1 BAP and NaCl concentrations of 0, 6, 7, 8, 9 g.l -1 for 2 months .Then, living buds were transferred to medium without salt. After one month, we repeated the first stage. All living buds rooted and were transferred to potted soil. Acclimatized plants were irrigated weekly with above NaCl solution. Other irrigation was done with salt-free water. There was also a negative relation between salt concentration and survival - proliferation. In second salinity stress, salt had no significant difference on survival percentage. No-significant difference of effect salt on survival in second salinity stress was observed. (author)

  14. A versatile, non genetically modified organism (GMO)-based strategy for controlling low-producer mutants in Bordetella pertussis cultures using antigenic modulation.

    Science.gov (United States)

    Goffin, Philippe; Slock, Thomas; Smessaert, Vincent; De Rop, Philippe; Dehottay, Philippe

    2015-08-01

    The uncontrolled presence of non-producer mutants negatively affects bioprocesses. In Bordetella pertussis cultures, avirulent mutants emerge spontaneously and accumulate. We characterized the dynamics of accumulation using high-throughput growth assays and competition experiments between virulent and avirulent (bvg(-) ) isolates. A fitness advantage of bvg(-) cells was identified as the main driver for bvg(-) accumulation under conditions of high virulence factor production. Conversely, under conditions that reduce their expression (antigenic modulation), bvg(-) takeover could be avoided. A control strategy was derived, which consists in applying modulating conditions whenever virulence factor production is not required. It has a wide range of applications, from routine laboratory operations to vaccine manufacturing, where pertussis toxin yields were increased 1.4-fold by performing early pre-culture steps in modulating conditions. Because it only requires subtle modifications of the culture medium and does not involve genetic modifications, this strategy is applicable to any B. pertussis isolate, and should facilitate regulatory acceptance of process changes for vaccine production. Strategies based on the same concept, could be derived for other industrially relevant micro-organisms. This study illustrates how a sound scientific understanding of physiological principles can be turned into a practical application for the bioprocess industry, in alignment with Quality by Design principles. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Effect of culture medium on polymer production and temperature on recovery of polymer produced from newly identified Rhyzopus oryzae ST29

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    Tipparat Hongpattarakere

    2008-04-01

    Full Text Available Thermotolerant fungal isolate ST29 was identified by observing on cell morphology and molecular technique based on internal transcribed spacer (ITS gene to be Rhizopus oryzae. Among four culture media tested, the strain exhibited the highest growth in yeast malt extract (YM medium (4.87 g/l, followed by Sabouraud dextrose broth (SDB (4.25 g/l, potato dextrose broth (PDB (4.10 g/l and palm oil mill effluent (POME (3.29 g/l, respectively, after 4 days cultivation at 45oC. However, the strain was found to produce polymer only in POME medium at 45oC, but not in the three synthetic media tested. Effect of temperature on separation of the biopolymer produced by this fungal strain was studied by incubating the culture broth in water bath with temperatures in the range of room temperature to 70oC. The biopolymer was recovered by filtration, centrifugation, and precipitation by adding 4 volumes of 95% ethanol, then freeze-drying. These temperatures therefore had no influence on the biopolymer yields (5.58-5.78 g/l or on biomass yields (2.90-3.29 g/l.

  16. Urine culture

    Science.gov (United States)

    Culture and sensitivity - urine ... when urinating. You also may have a urine culture after you have been treated for an infection. ... when bacteria or yeast are found in the culture. This likely means that you have a urinary ...

  17. Experimental investigation on spontaneously active hippocampal cultures recorded by means of high-density MEAs: analysis of the spatial resolution effects

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    Alessandro Maccione

    2010-05-01

    Full Text Available Based on experiments performed with high-resolution Active Pixel Sensor microelectrode arrays (APS-MEAs coupled with spontaneously active hippocampal cultures, this work investigates the spatial resolution effects of the neuroelectronic interface on the analysis of the recorded electrophysiological signals. The adopted methodology consists, first, in recording the spontaneous activity at the highest spatial resolution (inter-electrode separation of 21 µm from the whole array of 4096 microelectrodes. Then, the full resolution dataset is spatially down sampled in order to evaluate the effects on raster plot representation, array-wide spike rate (AWSR, mean firing rate (MFR and mean bursting rate (MBR. Furthermore, the effects of the array-to-network relative position are evaluated by shifting a subset of equally spaced electrodes on the entire recorded area. Results highlight that MFR and MBR are particularly influenced by the spatial resolution provided by the neuroelectronic interface. On high-resolution large MEAs, such analysis better represent the time-based parameterization of the network dynamics. Finally, this work suggest interesting capabilities of high-resolution MEAs for spatial-based analysis in dense and low-dense neuronal preparation for investigating signalling at both local and global neuronal circuitries.

  18. Screening of endophytic sources of exopolysaccharides: Preliminary characterization of crude exopolysaccharide produced by submerged culture of Diaporthe sp. JF766998 under different cultivation time

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    Ravely Casarotti Orlandelli

    2016-06-01

    Full Text Available Endophytic fungi have been described as producers of important bioactive compounds; however, they remain under-exploited as exopolysaccharides (EPS sources. Therefore, this work reports on EPS production by submerged cultures of eight endophytes isolated from Piper hispidum Sw., belonging to genera Diaporthe, Marasmius, Phlebia, Phoma, Phyllosticta and Schizophyllum. After fermentation for 96 h, four endophytes secreted EPS: Diaporthe sp. JF767000, Diaporthe sp. JF766998, Diaporthe sp. JF767007 and Phoma herbarum JF766995. The EPS from Diaporthe sp. JF766998 differed statistically from the others, with a higher percentage of carbohydrate (91% and lower amount of protein (8%. Subsequently, this fungus was grown under submerged culture for 72, 96 and 168 h (these EPS were designated EPSD1-72, EPSD1-96 and EPSD1-168 and the differences in production, monosaccharide composition and apparent molecular were compared. The EPS yields in mg/100 mL of culture medium were: 3.0 ± 0.4 (EPSD1-72, 15.4 ± 2.2 (EPSD1-96 and 14.8 ± 1.8 (EPSD1-168. The EPSD1-72 had high protein content (28.5% and only 71% of carbohydrate; while EPSD1-96 and EPSD1-168 were composed mainly of carbohydrate (≈95 and 100%, respectively, with low protein content (≈5% detected at 96 h. Galactose was the main monosaccharide component (30% of EPSD1-168. Differently, EPSD1-96 was rich in glucose (51%, with molecular weight of 46.6 kDa. It is an important feature for future investigations, because glucan-rich EPS are reported as effective antitumor agents.

  19. Preparation of Biocomposites using Sawdust and Lignosulfonate with a Culturе Liquid of Levan Producer Azotobacter vinelandii as a Bonding Agent

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    Victor V. Revin

    2016-02-01

    Full Text Available The possibility of preparing molding-bioengineered materials, such as woodchip boards (WCB, from sawdust using technical lignosulfonate (LGS, a wood waste product, and a culture liquid (CL of levan microbial polysaccharide producer by Azotobacter vinelandii D-08 is explored in this article. The parameters of the derived materials are comparable to those of traditional materials made from toxic phenol-formaldehyde resins. The various physical and mechanical characteristics of the materials depend on the quantity of the bonding agent used for the preparation. Adding a culture liquid increases the humidity resistance of the molding materials. Using electron microscopy and X-ray micro-tomography, it is clear that the structure of woodchip boards become more homogeneous without microcracks with the addition of CL. The strength of the best samples prepared was approximately 24 to 29 MPa with a density of 1170 to 1255 kg/m3 and a swell on wetting of 6.7%. During hot pressing, noticeable changes were observed by Fourier transform infrared spectroscopy (FTIR at frequencies typical of LGS sulfonic-acid groups, levan fructose fragments, and skeletal vibrations of a syringal/guaiacyl core in lignin and of C-H groups of hemicelluloses. This indicates the involvement of these functional groups in the process of binding wood particles with hot pressing.

  20. Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF

    Directory of Open Access Journals (Sweden)

    Coppock Donald L

    2008-12-01

    Full Text Available Abstract Background Multiple physiologic impairments are responsible for chronic wounds. A cell line grown which retains its phenotype from patient wounds would provide means of testing new therapies. Clinical information on patients from whom cells were grown can provide insights into mechanisms of specific disease such as diabetes or biological processes such as aging. The objective of this study was 1 To culture human cells derived from patients with chronic wounds and to test the effects of putative therapies, Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF on these cells. 2 To describe a methodology to create fibroblast cell lines from patients with chronic wounds. Methods Patient biopsies were obtained from 3 distinct locations on venous ulcers. Fibroblasts derived from different wound locations were tested for their migration capacities without stimulators and in response to GM-CSF. Another portion of the patient biopsy was used to develop primary fibroblast cultures after rigorous passage and antimicrobial testing. Results Fibroblasts from the non-healing edge had almost no migration capacity, wound base fibroblasts were intermediate, and fibroblasts derived from the healing edge had a capacity to migrate similar to healthy, normal, primary dermal fibroblasts. Non-healing edge fibroblasts did not respond to GM-CSF. Six fibroblast cell lines are currently available at the National Institute on Aging (NIA Cell Repository. Conclusion We conclude that primary cells from chronic ulcers can be established in culture and that they maintain their in vivo phenotype. These cells can be utilized for evaluating the effects of wound healing stimulators in vitro.

  1. Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing Bacterium Bacillus megaterium SP1 and Its Application in Aquaculture Wastewater Treatment.

    Science.gov (United States)

    Luo, Liang; Zhao, Zhigang; Huang, Xiaoli; Du, Xue; Wang, Chang'an; Li, Jinnan; Wang, Liansheng; Xu, Qiyou

    2016-01-01

    A bioflocculant-producing bacterium, Bacillus megaterium SP1, was isolated from biofloc in pond water and identified by using both 16S rDNA sequencing analysis and a Biolog GEN III MicroStation System. The optimal carbon and nitrogen sources for Bacillus megaterium SP1 were 20 g L -1 of glucose and 0.5 g L -1 of beef extract at 30°C and pH 7. The bioflocculant produced by strain SP1 under optimal culture conditions was applied into aquaculture wastewater treatment. The removal rates of chemical oxygen demand (COD), total ammonia nitrogen (TAN), and suspended solids (SS) in aquaculture wastewater reached 64, 63.61, and 83.8%, respectively. The volume of biofloc (FV) increased from 4.93 to 25.97 mL L -1 . The addition of Bacillus megaterium SP1 in aquaculture wastewater could effectively improve aquaculture water quality, promote the formation of biofloc, and then form an efficient and healthy aquaculture model based on biofloc technology.

  2. Comparison of dairy desserts produced with a potentially probiotic mixed culture and dispersions obtained from Gracilaria birdiae and Gracilaria domingensis seaweeds used as thickening agents.

    Science.gov (United States)

    Tavares Estevam, Adriana Carneiro; de Almeida, Michele Correia; de Oliveira, Tiago Almeida; Florentino, Eliane Rolim; Alonso Buriti, Flávia Carolina; Porto, Ana Lúcia Figueiredo

    2017-09-20

    Dairy desserts have emerged as interesting options for the incorporation of probiotics, bioactive ingredients and alternative sources of thickeners. This shows an opportunity to investigate the use of Gracilaria seaweeds in the formulation of potentially probiotic dairy desserts. This study aimed to compare the effects of dispersions obtained from Gracilaria domingensis and Gracilaria birdiae used as thickening agents on texture properties of dairy desserts fermented with SAB 440-A, composed of the starter Streptococcus thermophilus and the potential probiotics Bifidobacterium animalis and Lactobacillus acidophilus, and also to study their physicochemical characteristics, microbial viability and sensory acceptability. No significant differences between desserts with G. birdiae or G. domingensis dispersions regarding total solids, ash and fat content, as well as pH, titratable acidity, the viability of the microorganisms of the mixed culture and sensory acceptability were verified (P > 0.05). Nonetheless, the dessert with G. domingensis dispersion showed higher dietary fibre content and significantly increased firmness than the one produced with G. birdiae (P desserts, in the presence of either G. birdiae or G. domingensis dispersions, despite the fact that L. acidophilus has shown low viability in the final products. Therefore, the G. domingensis dispersion is suitable to be used as a thickening agent to produce dairy desserts with enhanced firmness and good sensory acceptability, it being also advisable to use only B. animalis as a probiotic for this product.

  3. MEANS FOR PRODUCING PLUTONIUM CHAIN REACTIONS

    Science.gov (United States)

    Wigner, E.P.; Weinberg, A.M.

    1961-01-24

    A neutronic reactor is described with an active portion capable of operating at an energy level of 0.5 to 1000 ev comprising discrete bodies of Pu/ sup 239/ disposed in a body of water which contains not more than 5 molecules of water to one atom of plutonium, the total amount of Pu/sup 239/ being sufficient to sustain a chain reaction. (auth)

  4. Chromogenic culture media or rapid immunochromatographic test: Which is better for detecting Klebsiella pneumoniae that produce OXA-48 and can they be used in blood and urine specimens.

    Science.gov (United States)

    Genc, Ozlem; Aksu, Evrim

    2018-05-01

    Our goal was to compare a rapid test (OXA-48K-SeT) and four different chromogenic media (CHROMagar KPC, CHROMagar mSuperCARBA, ChromID Carba and ChromID OXA-48) for the detection of OXA-48 producing Klebsiella pneumoniae isolates and spiked urine/blood samples with these bacteria. In total 100 K.pneumoniae isolates, including 60 OXA-48 positive, 15 other carbapenemase producing, 15 Extended spectrum betalactamases (ESBL) positive and 10 carbapenem sensitive K.pneumoniae were included in the study. After all samples were inoculated into all chromogenic media, temocillin discs were placed onto the media. OXA-48K-SeT was studied according to the manufacturer's instructions and the lower detection limit was determined. Sensitivities and specificities of all chromogenic media and rapid test were detected as 100%. All of the OXA-48 producers were found resistant to temocillin on all chromogenic media. The lower detection limit of the rapid assay was determined as 10 6 in both direct bacterial samples and in spiked urine/blood samples. As a result, four chromogenic culture media and OXA-48 K-SeT can be used safely for detection of OXA-48 positive K.pneumoniae isolates. Although direct clinical specimens were not used, our study suggests that this media and OXA-48 K-SeT may be used in patient samples like blood and urine. Further studies are needed to assess this suggestion. Copyright © 2018 Elsevier B.V. All rights reserved.

  5. Culturing Protozoa.

    Science.gov (United States)

    Stevenson, Paul

    1980-01-01

    Compares various nutrient media, growth conditions, and stock solutions used in culturing protozoa. A hay infusion in Chalkey's solution maintained at a stable temperature is recommended for producing the most dense and diverse cultures. (WB)

  6. HOW A TOURIST DESTINATION MAY BECOME A BRAND BY MEANS OF EVENTS – A CASE STUDY ON IASI AS A CANDIDATE FOR EUROPEAN CULTURAL CAPITAL 2021

    Directory of Open Access Journals (Sweden)

    Ancuta-Mihaela ZETIU

    2015-10-01

    Full Text Available This paper presents an analysis on the impact generated by hosting the International Festival of Education, 2013 edition, on Iasi’s image and its contribution in the competition for winning the title "European Capital of Culture 2021". The article reviews aspects related to culture, events and city branding, the strategy and the effects of hosting a cultural event on the host city. Cultural event was discussed as a tool used for urban regeneration and branding a tourist destination. Moreover, statistics show how the event contributed to increase tourism in Iasi.

  7. Transmission of HCV to a chimpanzee using virus particles produced in an RNA-transfected HepG2 cell culture.

    Science.gov (United States)

    Dash, S; Kalkeri, G; McClure, H M; Garry, R F; Clejan, S; Thung, S N; Murthy, K K

    2001-10-01

    It was demonstrated previously that HepG2 cells produce negative strand RNA and virus-like particles after transfection with RNA transcribed from a full-length hepatitis C virus (HCV) cDNA clone [Dash et al. (1997) American Journal of Pathology, 151:363-373]. To determine in vivo infectivity of these in vitro synthesized viral particles, a chimpanzee was inoculated intravenously with HCV derived from HepG2 cells. The infected chimpanzee was examined serially for elevation of liver enzymes, for the presence of HCV RNA in the serum by reverse transcription nested polymerase chain reaction (RT-PCR), anti-HCV antibodies in the serum, and inflammation in the liver. The chimpanzee developed elevated levels of liver enzymes after the second week, but the levels fluctuated over a 10-week period. HCV RNA was detected in the serum of the chimpanzee at the second, seventh and ninth weeks after inoculation, and remained positive up to 25 weeks. Liver biopsies at Weeks 18 and 19 revealed of mild inflammation. Nucleotide sequence analysis of HCV recovered from the infected chimpanzee at the second and ninth weeks showed 100% sequence homology with the clone used for transfection studies. Serum anti-HCV antibodies were not detected by EIA during the 25 weeks follow-up period. These results suggest that intravenous administration of the virus-like particles derived from RNA-transfected HepG2 cells are infectious, and therefore, the pMO9.6-T7 clone is an infectious clone. These results provide new information that in vitro synthesized HCV particles produced from full-length HCV clone can cause infection in a chimpanzee. This study will facilitate the use of innovative approaches to the study of assembly of HCV particles and mechanisms of virus infectivity in cell culture. Copyright 2001 Wiley-Liss, Inc.

  8. A comparison of self-assembly and hydrogel encapsulation as a means to engineer functional cartilaginous grafts using culture expanded chondrocytes.

    Science.gov (United States)

    Mesallati, Tariq; Buckley, Conor T; Kelly, Daniel J

    2014-01-01

    Despite an increased interest in the use of hydrogel encapsulation and cellular self-assembly (often termed "self-aggregating" or "scaffold-free" approaches) for tissue-engineering applications, to the best of our knowledge, no study to date has been undertaken to directly compare both approaches for generating functional cartilaginous grafts. The objective of this study was to directly compare self-assembly (SA) and agarose hydrogel encapsulation (AE) as a means to engineer such grafts using passaged chondrocytes. Agarose hydrogels (5 mm diameter × 1.5 mm thick) were seeded with chondrocytes at two cell seeding densities (900,000 cells or 4 million cells in total per hydrogel), while SA constructs were generated by adding the same number of cells to custom-made molds. Constructs were either supplemented with transforming growth factor (TGF)-β3 for 6 weeks, or only supplemented with TGF-β3 for the first 2 weeks of the 6 week culture period. The SA method was only capable of generating geometrically uniform cartilaginous tissues at high seeding densities (4 million cells). At these high seeding densities, we observed that total sulphated glycosaminoglycan (sGAG) and collagen synthesis was greater with AE than SA, with higher sGAG retention also observed in AE constructs. When normalized to wet weight, however, SA constructs exhibited significantly higher levels of collagen accumulation compared with agarose hydrogels. Furthermore, it was possible to engineer such functionality into these tissues in a shorter timeframe using the SA approach compared with AE. Therefore, while large numbers of chondrocytes are required to engineer cartilaginous grafts using the SA approach, it would appear to lead to the faster generation of a more hyaline-like tissue, with a tissue architecture and a ratio of collagen to sGAG content more closely resembling native articular cartilage.

  9. Decolorization of the azo dye Acid Orange 51 by laccase produced in solid culture of a newly isolated Trametes trogii strain.

    Science.gov (United States)

    Daâssi, Dalel; Zouari-Mechichi, Hela; Frikha, Fakher; Martinez, Maria Jesus; Nasri, Moncef; Mechichi, Tahar

    2013-04-01

    This study concerns the decolorization and detoxification of the azo dye Acid Orange 51 (AO51) by crude laccase from Trametes trogii produced in solid culture using sawdust as support media. A three-level Box-Behnken factorial design with four factors (enzyme concentration, 1-hydroxybenzotriazole (HBT) concentration, dye concentration and reaction time) combined with response surface methodology was applied to optimize AO51 decolorization. A mathematical model was developed showing the effect of each factor and their interactions on color removal. The model predicted that Acid Orange 51 decolorization above 87.87 ± 1.27 % could be obtained when enzyme concentration, HBT concentration, dye concentration and reaction time were set at 1 U/mL, 0.75 mM, 60 mg/L and 2 days, respectively. The experimental values were in good agreement with the predicted ones and the models were highly significant, the correlation coefficient (R 2 ) being 0.9. Then the desirability function was employed to determine the optimal decolorization condition for each dye and minimize the process cost simultaneously. In addition, germination index assay showed that laccase-treated dye was detoxified; however in the presence of HBT, the phytotoxicity of the treated dye was increased. By using cheap agro-industrial wastes, such as sawdust, a potential laccase was obtained. The low cost of laccase production may further broaden its application in textile wastewater treatment.

  10. Growth reduction of Listeria spp. caused by undefined industrial red smear cheese cultures and bacteriocin-producing Brevibacterium lines as evaluated in situ on soft cheese.

    Science.gov (United States)

    Eppert, I; Valdés-Stauber, N; Götz, H; Busse, M; Scherer, S

    1997-01-01

    The undefined microbial floras derived from the surface of ripe cheese which are used for the ripening of commercial red smear cheeses have a strong impact on the growth of Listeria spp. In some cases, these microbial consortia inhibit Listeria almost completely. From such undefined industrial cheese-ripening floras, linocin M18-producing (lin+) (N. Valdés-Stauber and S. Scherer, Appl. Environ. Microbiol. 60:3809-3814, 1994) and -nonproducing Brevibacterium linens strains were isolated and used as single-strain starter cultures on model red smear cheeses to evaluate their potential inhibitory effects on Listeria strains in situ. On cheeses ripened with lin+ strains, a growth reduction of L. ivanovii and L. monocytogenes of 1 to 2 log units was observed compared to cheeses ripened with lin strains. Linocin M18 activity was detected in cheeses ripened with lin+ strains but was not found in those ripened with lin strains. We suggest that production of linocin M18 contributes to the growth reduction of Listeria observed on model red smear cheeses but is unsufficient to explain the almost complete inhibition of Listeria caused by some undefined microbial floras derived from the surface of ripe cheeses. PMID:9406400

  11. Atividade alelopática do filtrado de cultura produzido por Fusarium solani Allelopathic activity of culture filtrate produced by Fusarium solani

    Directory of Open Access Journals (Sweden)

    A.P.S. Souza Filho

    2007-03-01

    germination and radicle and hypocotyl elongation of the weeds Mimosa pudica and Senna obtusifolia. The results showed potential inhibitory allelopathic activity of the Fusarium culture filtrate, varying according to concentration and receiving plants. The intensity of the inhibition effects promoted by the extracts was clearly associated to concentration, with the major effect being observed at 4%. Regardless of concentration and bioassays, Mimosa pudica was more sensitive to the toxin effects of the culture filtrate. Radicle elongation was more intensely inhibited by the culture filtrate toxins. The results showed potential for the use of the toxins produced by Fusarium solani f. sp. pipers, as an alternative source to control weeds. However, further studies should be carried out.

  12. Infectivity of Oryctes Nudivirus Produced on Cell Culture Dsir Ha-1179 Against Larvae and Its Effects on Feeding of Neonates of Rhinoceros Beetle, Oryctes Rhinoceros

    International Nuclear Information System (INIS)

    Nur Ain Farhah Ros Saidon Khudri; Wahizatul Afzan Azmi; Ramle Moslim; Norman Kamarudin; Siti Ramlah Ahmad Ali

    2016-01-01

    The Oryctes nudivirus (OrNV) is a classical biocontrol agent for a major oil palm insect pest the rhinoceros beetle, Oryctes rhinoceros. The infectivity of three Malaysian indigenous types of OrNV types A, B and C were tested on larvae and neonates. On larvae, the peroral inoculation test technique indicated that the highest mortality of 100 % was achieved using type A produced from cell culture DSIR-HA-1179, while the highest infectivity of 41.7 % was recorded for type A prepared from infected guts. No differences in infectivity were observed on other treatments, which ranged from 13.1 % to 41.7 %. In the substrate contamination inoculation test technique, results showed that the level of infectivity was even lower in all OrNV treatments, ranging as low as 6.7 % to only 15.0 %. Low infectivity was mainly due to inactivation of virus inocula in the larval food substrates. Based on the results for both inoculation methods, the OrNV type C prepared from cell culture DSIR-HA-1179 was found more effective in controlling the L3 larvae than the other types of OrNV. The impact of OrNV infection on food consumption by the neonates was studied. The feeding of inoculated neonates with OrNV reduced rapidly, especially at the early stage of the experiment between eight days after treatment (DAT) to 16 DAT. At this period, the food consumption by all tested OrNV was rapidly reduced and maintained low until the experiment ended at 60 DAT. The highest feeding reduction rate was on neonates treated by type A (-0.074x) followed by neonates treated by type C (-0.053x) and type B (-0.035x). Therefore, it was suggested that besides on highly virulent, the selection of OrNV for field release should also based on high reduction rate on food consumption by the infected insects on plant hosts. (author)

  13. The formation of bases of culture of movement at children of 3–6 years old by means of rhythmic gymnastics

    Directory of Open Access Journals (Sweden)

    Olga Riabchenko

    2015-06-01

    Full Text Available Purpose: to open the versatility of a concept of culture as a phenomena and as a process which course provides the preservation of the most significant viable social relations conformably to a concept of culture of movements of children of 3-6 years old. Material and Methods: the analysis of special scientific literature in which theoretical and practical aspects of the creation of physical culture are stated. Results: questions of the creation of physical training of children of 3–6 years old taking into account their individual physical developments are considered. The most important provisions are noted in the structure of physical culture which doesn’t allow solving this problem with a full scientific validity now. Conclusions: the basic provisions are allocated which decision is a paramount task for the creation of the evidence-based system of physical training of children of the advanced preschool age.

  14. A Simplified Method for Three-Dimensional (3-D Ovarian Tissue Culture Yielding Oocytes Competent to Produce Full-Term Offspring in Mice.

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    Carolyn M Higuchi

    Full Text Available In vitro growth of follicles is a promising technology to generate large quantities of competent oocytes from immature follicles and could expand the potential of assisted reproductive technologies (ART. Isolated follicle culture is currently the primary method used to develop and mature follicles in vitro. However, this procedure typically requires complicated, time-consuming procedures, as well as destruction of the normal ovarian microenvironment. Here we describe a simplified 3-D ovarian culture system that can be used to mature multilayered secondary follicles into antral follicles, generating developmentally competent oocytes in vitro. Ovaries recovered from mice at 14 days of age were cut into 8 pieces and placed onto a thick Matrigel drop (3-D culture for 10 days of culture. As a control, ovarian pieces were cultured on a membrane filter without any Matrigel drop (Membrane culture. We also evaluated the effect of activin A treatment on follicle growth within the ovarian pieces with or without Matrigel support. Thus we tested four different culture conditions: C (Membrane/activin-, A (Membrane/activin+, M (Matrigel/activin-, and M+A (Matrigel/activin+. We found that the cultured follicles and oocytes steadily increased in size regardless of the culture condition used. However, antral cavity formation occurred only in the follicles grown in the 3-D culture system (M, M+A. Following ovarian tissue culture, full-grown GV oocytes were isolated from the larger follicles to evaluate their developmental competence by subjecting them to in vitro maturation (IVM and in vitro fertilization (IVF. Maturation and fertilization rates were higher using oocytes grown in 3-D culture (M, M+A than with those grown in membrane culture (C, A. In particular, activin A treatment further improved 3-D culture (M+A success. Following IVF, two-cell embryos were transferred to recipients to generate full-term offspring. In summary, this simple and easy 3-D ovarian

  15. Fermentative capabilities and volatile compounds produced by Kloeckera/Hanseniaspora and Saccharomyces yeast strains in pure and mixed cultures during Agave tequilana juice fermentation.

    Science.gov (United States)

    González-Robles, Ivonne Wendolyne; Estarrón-Espinosa, Mirna; Díaz-Montaño, Dulce María

    2015-09-01

    The fermentative and aromatic capabilities of Kloeckera africana/Hanseniaspora vineae K1, K. apiculata/H. uvarum K2, and Saccharomyces cerevisiae S1 and S2 were studied in pure and mixed culture fermentations using Agave tequila juice as the culture medium. In pure and mixed cultures, Kloeckera/Hanseniaspora strains showed limited growth and sugar consumption, as well as low ethanol yield and productivity, compared to S. cerevisiae, which yielded more biomass, ethanol and viable cell concentrations. In pure and mixed cultures, S. cerevisiae presented a similar behaviour reaching high biomass production, completely consuming the sugar, leading to high ethanol production. Furthermore, the presence of S. cerevisiae strains in the mixed cultures promoted the production of higher alcohols, acetaldehyde and ethyl esters, whereas Kloeckera/Hanseniaspora strains stimulated the production of ethyl acetate and 2-phenyl ethyl acetate compounds.

  16. Kinetics of early in vitro development of bovine in vivo- and in vitro-derived zygotes produced and/or cultured in chemically defined or serum-containing media

    DEFF Research Database (Denmark)

    Holm, P; Booth, P J; Callesen, H

    2002-01-01

    The kinetics of the in vitro development of early embryos from bovine zygotes derived in vitro and in vitro were compared, investigating the effect of serum during in vitro maturation and fertilization (IVM-IVF) and in culture. Zygotes were collected from superovulated heifers or produced in vitro...... to the compact morula or blastocyst stages (87% versus 47-54 respectively; P

  17. High cell density suppresses BMP4-induced differentiation of human pluripotent stem cells to produce macroscopic spatial patterning in a unidirectional perfusion culture chamber.

    Science.gov (United States)

    Tashiro, Shota; Le, Minh Nguyen Tuyet; Kusama, Yuta; Nakatani, Eri; Suga, Mika; Furue, Miho K; Satoh, Taku; Sugiura, Shinji; Kanamori, Toshiyuki; Ohnuma, Kiyoshi

    2018-04-19

    Spatial pattern formation is a critical step in embryogenesis. Bone morphogenetic protein 4 (BMP4) and its inhibitors are major factors for the formation of spatial patterns during embryogenesis. However, spatial patterning of the human embryo is unclear because of ethical issues and isotropic culture environments resulting from conventional culture dishes. Here, we utilized human pluripotent stem cells (hiPSCs) and a simple anisotropic (unidirectional perfusion) culture chamber, which creates unidirectional conditions, to measure the cell community effect. The influence of cell density on BMP4-induced differentiation was explored during static culture using a conventional culture dish. Immunostaining of the early differentiation marker SSEA-1 and the mesendoderm marker BRACHYURY revealed that high cell density suppressed differentiation, with small clusters of differentiated and undifferentiated cells formed. Addition of five-fold higher concentration of BMP4 showed similar results, suggesting that suppression was not caused by depletion of BMP4 but rather by high cell density. Quantitative RT-PCR array analysis showed that BMP4 induced multi-lineage differentiation, which was also suppressed under high-density conditions. We fabricated an elongated perfusion culture chamber, in which proteins were transported unidirectionally, and hiPSCs were cultured with BMP4. At low density, the expression was the same throughout the chamber. However, at high density, SSEA-1 and BRACHYURY were expressed only in upstream cells, suggesting that some autocrine/paracrine factors inhibited the action of BMP4 in downstream cells to form the spatial pattern. Human iPSCs cultured in a perfusion culture chamber might be useful for studying in vitro macroscopic pattern formation in human embryogenesis. Copyright © 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  18. In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence.

    Science.gov (United States)

    Gerger, R P C; Ribeiro, E S; Forell, F; Bertolini, L R; Rodrigues, J L; Ambrósio, C E; Miglino, M A; Mezzalira, A; Bertolini, M

    2010-02-18

    The relationship between the level of cell confluence near the plateau phase of growth and blastocyst yield following somatic cell cloning is not well understood. We examined the effect of distinct cell culture confluence levels on in vitro development of cloned bovine embryos. In vitro-matured bovine oocytes were manually bisected and selected by DNA staining. One or two enucleated hemi-cytoplasts were paired and fused with an adult skin somatic cell. Cultured skin cells from an adult Nellore cow harvested at three distinct culture confluence levels (70-80, 80-90, and >95%) were used for construction of embryos and hemi-embryos. After activation, structures were cultured in vitro as one embryo (1 x 100%) or as aggregates of two hemi-embryos (2 x 50%) per microwell. Fusion, cleavage and blastocyst rates were compared using the chi(2) test. The fusion rate for hemi-embryos (51.4%) was lower than for embryos (67.6%), with no influence of degree of cell confluence. However, blastocyst rates improved linearly (7.0, 17.5, and 29.4%) with increases in cell confluence. We conclude that degree of cell culture confluence significantly influences subsequent embryo development; use of a cell population in high confluence (>90%) for nuclear transfer significantly improved blastocyst yield after cloning.

  19. Use of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR

    Directory of Open Access Journals (Sweden)

    Hatıra Taşkın

    2013-01-01

    Full Text Available This study was performed for comparison of meristem culture technique with shoot tip culture technique for obtaining virus-free plant, comparison of micropropagation success of two different nutrient media, and determination of effectiveness of real-time PCR assay for the detection of viruses. Two different garlic species (Allium sativum and Allium tuncelianum and two different nutrient media were used in this experiment. Results showed that Medium 2 was more successful compared to Medium 1 for both A. tuncelianum and A. sativum (Kastamonu garlic clone. In vitro plants obtained via meristem and shoot tip cultures were tested for determination of onion yellow dwarf virus (OYDV and leek yellow stripe virus (LYSV through real-time PCR assay. In garlic plants propagated via meristem culture, we could not detect any virus. OYDV and LYSV viruses were detected in plants obtained via shoot tip culture. OYDV virus was observed in amount of 80% and 73% of tested plants for A. tuncelianum and A. sativum, respectively. LYSV virus was found in amount of 67% of tested plants of A. tuncelianum and in amount of 87% of tested plants of A. sativum in this study.

  20. Acculturative stressor and meaning of life as predictors of negative affect in acculturation: a cross-cultural comparative study between Chinese international students in Australia and Hong Kong.

    Science.gov (United States)

    Pan, Jia-Yan; Wong, Daniel Fu Keung; Joubert, Lynette; Chan, Cecilia Lai Wan

    2007-09-01

    The purpose of the present study was to compare the predictive effects of acculturative stressor and meaning of life on negative affect in the process of acculturation between Chinese international students in Australia and Hong Kong. Four hundred mainland Chinese students studying at six universities in Hong Kong and 227 Chinese international students studying at the University of Melbourne in Australia completed a questionnaire that included measures of acculturative stressor, meaning of life, negative affect and demographic information. The Australian sample was found to have a higher level of acculturative stressor and negative affect than the Hong Kong sample. Acculturative stressor had a positive impact on negative affect in both samples, but the impact of different domains of acculturative stressor on negative affect varied between the two groups. Finally, meaning of life partially mediated the relationship between acculturative stressor and negative affect in the Hong Kong sample, but no such effect was found in the Australia sample. Acculturative stressor is a critical risk factor for negative affect in acculturation for Chinese international students in Australia and Hong Kong. Meaning of life acted as a protective factor that mitigated negative affect for mainland Chinese students in Hong Kong, but not for the Chinese international students in Australia. The theoretical and practical implications for resilience-based and meaning-oriented intervention for Chinese international students are discussed.

  1. The Meaning of Meaning, Etc.

    Science.gov (United States)

    Nilsen, Don L. F.

    This paper attempts to dispel a number of misconceptions about the nature of meaning, namely that: (1) synonyms are words that have the same meanings, (2) antonyms are words that have opposite meanings, (3) homonyms are words that sound the same but have different spellings and meanings, (4) converses are antonyms rather than synonyms, (5)…

  2. Comparative study of predatory responses in blue mussels (Mytilus edulis L.) produced in suspended long line cultures or collected from natural bottom mussel beds

    DEFF Research Database (Denmark)

    Christensen, Helle Torp; Dolmer, Per; Petersen, Jens Kjerulf

    2011-01-01

    Blue mussels (Mytilus edulis L.) are a valuable resource for commercial shellfish production and may also have uses as a tool in habitat improvement, because mussel beds can increase habitat diversity and complexity. A prerequisite for both commercial mussel production and habitat improvement...... originated from suspended cultures had a higher length increment and lower mortality when compared to bottom mussels. It is concluded that suspended mussels potentially are an alternative resource to bottom culture and can be used in habitat improvement of mussel beds, but that the use of suspended mussels...

  3. Meaning in life experience at the end of life: validation of the Hindi version of the Schedule for Meaning in Life Evaluation and a cross-cultural comparison between Indian and German palliative care patients.

    Science.gov (United States)

    Kudla, Dorothea; Kujur, Julius; Tigga, Sumanti; Tirkey, Prakash; Rai, Punita; Fegg, Martin Johannes

    2015-01-01

    The experience of Meaning in Life (MiL) is a major protective factor against feelings of hopelessness and wishes for hastened death in palliative care (PC) patients. However, most instruments for MiL assessment have been developed only in Western countries so far. Little is known about MiL experience in Asian PC patients. This study aimed to provide a Hindi version of the Schedule for Meaning in Life Evaluation (SMiLE), test its feasibility and validity in Indian PC patients, and compare the results with previous studies in Germany. Indian PC patients in a hospice for the destitute were eligible to participate in this cross-sectional study. In the SMiLE instrument, respondents individually listed MiL-giving areas before rating their satisfaction with and importance of these areas. Overall indices of satisfaction (IoS, range 0-100), weighting (IoW, range 0-100), and weighted satisfaction (IoWS, range 0-100) were calculated. A Hindi forward-backward translation of the SMiLE was made. Two hundred fifty-eight Indian PC patients took part in the study (response rate 93.5%). Convergent validity of the SMiLE was found with the World Health Organization Quality of Life-Brief version (r = 0.17; P = 0.008) and the Idler Index of Religiosity (public religiousness: r = 0.25, P < 0.001 and private religiousness: r = 0.29, P < 0.001). Indian PC patients' IoW was 65.8 ± 22.1, IoS 68.6 ± 17.4, and IoWS 70.2 ± 17.0. In multivariate analyses of covariance, they differed significantly from German PC patients only in IoW (IoW: 84.8 ± 11.5, P < 0.001; IoS: 70.2 ± 19.7; IoWS: 72.0 ± 19.4). Compared with Germans, Indians more often listed spirituality (P < 0.001) and social commitment (P < 0.001) and less often social relations (P = 0.008). Preliminary results indicate good feasibility and validity of the Hindi version of the SMiLE. MiL experience also seems to be a coping resource for Indian PC patients. Copyright © 2015 American Academy of Hospice

  4. Culture versus the law in the decision not to vaccinate children: meanings assigned by middle-class couples in São Paulo, Brazil.

    Science.gov (United States)

    Barbieri, Carolina Luisa Alves; Couto, Márcia Thereza; Aith, Fernando Mussa Abujamra

    2017-03-09

    This study aimed to learn how middle-class parents in the city of São Paulo, Brazil, interpreted the country's prevailing vaccination requirements, based on their experiences with vaccinating, selectively vaccinating, or not vaccinating their children. A qualitative approach was used with in-depth interviews. The analytical process was guided by content analysis and the theoretical framework of the anthropology of the law and morality. For parents that vaccinated, Brazil's culture of immunization outweighed the feeling of compliance with the law; for selective parents, selection of vaccines was not perceived as deviating from the law. In both, the act of vaccinating their children was a matter of moral status. Meanwhile, the non-vaccinators, counter to the legal perspective, attributed their choice to care for the child on grounds that mandatory vaccination was contrary to their way of life; they experienced a feeling of social coercion and fear of legal impositions. Vaccination is an important practice in public health, but it can reveal tensions and conflicts from normative systems, whether moral, cultural, or legal.

  5. Analysis of energy culture the Crambe (Crambe abyssinica Hochst) produced in no tillage; Analise energetica da cultura do Crambe (Crambe abyssinica Hochst) produzida em plantio direto

    Energy Technology Data Exchange (ETDEWEB)

    Jasper, Samir Paulo; Biaggioni, Marco Antonio Martin; Silva, Paulo Roberto Arbex; Seki, Andre Satoshi; Bueno, Osmar Carvalho de [Universidade Estadual Paulista (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas], E-mail: jasper@fca.unesp.br

    2010-07-01

    The objective of this study was to characterize the energy consumption in the deployment and conduct of the culture of Crambe abyssinica Hochst in no-tillage, at the Experimental Farm Farm belonging to the Faculty of Agronomic Sciences - UNESP, located in Botucatu - SP. Assessing the sustainability of the system were estimated, energy efficiency and culture of crambe. To survey the components of the structure of expenditures, we used coefficients Energetic in specialized literature. The structures of energy expenditure by type, source and form, demonstrated that the indirect energy participated with 65.03%, industrial sources, fossil and biological accounted for 68.80%, 23.67% and 11.30%, respectively. The forms have been introduced; chemical fertilizers contributed 44.31% and diesel to 23.04% to 67.35% of energy expenditure, showing the dependence of the system of energy sources and industrial fuels. The efficiency of the culture was found to be 9.98, indicating that for each calorie used in the production of crambe return was 8.98 units, the efficiency was above 38, demonstrating the high sustainability of the agricultural system to reach energy cultural exceeding 37 thousand MJ ha{sup -1}. (author)

  6. Exploration of the hydrogen producing potential of Rhodobacter capsulatus chemostat cultures: The application of deceleration-stat and gradient-stat methodology

    NARCIS (Netherlands)

    Hoekema, S.; Breukelen, van F.R.; Janssen, M.G.J.; Tramper, J.; Wijffels, R.H.

    2009-01-01

    In this work, the dependency of the volumetric hydrogen production rate of ammonium-limited Rhodobacter capsulatus chemostat cultures on their imposed biomass concentration and dilution rate was investigated. A deceleration-stat experiment was performed by lowering the dilution rate from 1.0 d-1 to

  7. Use of a genetically enhanced, pediocin-producing starter culture, Lactococcus lactis subsp. lactis MM217, to control Listeria monocytogenes in cheddar cheese

    NARCIS (Netherlands)

    Buyong, N; Kok, J; Luchansky, JB

    1998-01-01

    Cheddar cheese was prepared with Lactococcus lactis subsp, lactis MM217, a starter culture which contains pMC117 coding for pediocin PA-1, About 75 liters of pasteurized milk (containing ca, 3.6% fat) was inoculated with strain MM217 (ca, 10(6) CFU per ml) and a mixture of three Listeria

  8. The Ever Challenging Environment of Producing Knowledgeable and Skillful Graduates: Changing the Culture of Higher Education of Institutions in Teaching and Learning Approaches

    Science.gov (United States)

    Zakaria, Sabarudin

    2017-01-01

    The education system of modern society can be understood as an institution whose functions include its capacity to reproduce its host society, both economically and culturally. Higher institution of learning is no more seen as a place of mass production of graduates willingly believes that they are now ready to perform their duties. The newly…

  9. The Protective Function of Meaning of Life on Life Satisfaction among Chinese Students in Australia and Hong Kong: A Cross-Cultural Comparative Study

    Science.gov (United States)

    Pan, Jia-Yan; Wong, Daniel Fu Keung; Joubert, Lynette; Chan, Cecilia Lai Wan

    2008-01-01

    Objective: The authors compared the predictive effects of acculturative stressors and meaning of life on life satisfaction between Chinese students in Australia and in Hong Kong. Participants: In 2006, the researchers recruited 606 Chinese students studying abroad at the University of Melbourne in Australia and at 6 universities in Hong Kong.…

  10. Organizational changes and automation: By means of a customer-oriented policy the so-called 'island culture' disappears: Part 2

    International Nuclear Information System (INIS)

    Van Gelder, J.W.

    1994-01-01

    Automation offers great opportunities in the efforts of energy utilities in the Netherlands to reorganize towards more customer-oriented businesses. However, automation in itself is not enough. First, the organizational structure has to be changed considerably. Various energy utilities have already started on it. The restructuring principle is the same everywhere, but the way it is implemented differs widely. In this article attention is paid to different customer information systems. These systems can put an end to the so-called island culture within the energy utility organizations. The systems discussed are IRD of Systema and RIVA of SAP (both German software businesses), and two Dutch systems: Numis-2000 of Multihouse and KIS/400 of NUON Info-Systemen

  11. Use of Lactobacillus plantarum as Starter Culture and Its Influence on Physicochemical, Microbiological, and Sensory Characteristics of Kunnu-Aya Produced from Sorghum and Tigernut

    Directory of Open Access Journals (Sweden)

    O. A. Olaoye

    2017-01-01

    Full Text Available Kunun-aya is a traditional nonalcoholic beverage in the northern part of Nigeria, normally prepared from cereals. In this investigation Lactobacillus plantarum, isolated from fermenting kunun, was applied as starter culture during production of kunun-aya from varying combinations of sorghum and tigernut. The quality attributes of the product indicated increase in ash and protein contents of product inoculated with starter culture (PISC over the uninoculated control sample (UCS. The highest values of 4.43% and 6.95% were recorded for ash and protein, respectively, in the product from fifty percent each of sorghum and tigernut (50SOR/50TIG. Titratable acidity was higher in PISC compared to UCS; the 50SOR/50TIG sample had the highest value of 0.92. The PISC recorded reduced counts of Salmonella, coliforms, and Staphylococci. The SCIS were preferred by panellists in the sensory attributes of appearance, aroma, taste, mouthfeel, and general acceptability. It was concluded that the use of L. plantarum as starter culture in the production of kunun-aya was advantageous as a result of enhanced nutritional, sensory, and microbial qualities recorded compared to UCS. Reduction in Salmonella, coliforms, and Staphylococci in PISC may be of public health significance. This on quality improvement of the traditional beverage has not been previously reported.

  12. Optimization of a pH-shift control strategy for producing monoclonal antibodies in Chinese hamster ovary cell cultures using a pH-dependent dynamic model.

    Science.gov (United States)

    Hogiri, Tomoharu; Tamashima, Hiroshi; Nishizawa, Akitoshi; Okamoto, Masahiro

    2018-02-01

    To optimize monoclonal antibody (mAb) production in Chinese hamster ovary cell cultures, culture pH should be temporally controlled with high resolution. In this study, we propose a new pH-dependent dynamic model represented by simultaneous differential equations including a minimum of six system component, depending on pH value. All kinetic parameters in the dynamic model were estimated using an evolutionary numerical optimization (real-coded genetic algorithm) method based on experimental time-course data obtained at different pH values ranging from 6.6 to 7.2. We determined an optimal pH-shift schedule theoretically. We validated this optimal pH-shift schedule experimentally and mAb production increased by approximately 40% with this schedule. Throughout this study, it was suggested that the culture pH-shift optimization strategy using a pH-dependent dynamic model is suitable to optimize any pH-shift schedule for CHO cell lines used in mAb production projects. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  13. Detection of adenoviruses in shellfish by means of conventional-PCR, nested-PCR, and integrated cell culture PCR (ICC/PCR).

    Science.gov (United States)

    Rigotto, C; Sincero, T C M; Simões, C M O; Barardi, C R M

    2005-01-01

    We tested three PCR based methodologies to detect adenoviruses associated with cultivated oysters. Conventional-PCR, nested-PCR, and integrated cell culture-PCR (ICC/PCR) were first optimized using oysters seeded with know amounts of Adenovirus serotype 5 (Ad5). The maximum sensitivity for Ad5 detection was determined for each method, and then used to detect natural adenovirus contamination in oysters from three aquiculture farms in Florianopolis, Santa Catarina State, Brazil, over a period of 6 months. The results showed that the nested-PCR was more sensitive (limit of detection: 1.2 PFU/g of tissue) than conventional-PCR and ICC-PCR (limit of detection for both: 1.2 x 10(2)PFU/g of tissue) for detection of Ad5 in oyster extracts. Nested-PCR was able to detect 90% of Ad5 contamination in harvested oyster samples, while conventional-PCR was unable to detect Ad5 in any of the samples. The present work suggests that detection of human adenoviruses can be used as a tool to monitor the presence of human viruses in marine environments where shellfish grow, and that nested-PCR is the method of choice.

  14. Comparative investigations of the heavy-metal uptake of different culture plants from soils fertilized with sewage sludge and its forecasting by means of soil extraction

    International Nuclear Information System (INIS)

    Luebben, S.; Goettingen Univ.

    1993-01-01

    This report is part of a research programme sponsored by the Federal Ministry of Science and Technology entitled ''Impact of municipal wastes on soils, soil organisms and plants''. In field experiments or on samples from these fields (four partial projects), soil microbiological, soil zoological and other pedological investigations were carried through; furthermore, soil samples were taken in the areas of five other partial projects studying for many years the impact of municipal waste, in order to investigate heavy metal transfer from soil to plants in greater detail. As the evaluation of the rates of heavy metal elimination shows, the possibility of soil decontamination merely through heavy metal transfer to plants is practically excluded. In moderately contaminated areas those culture plants should be given priority whose useful parts take up relatively little heavy metal. By raising the pH-value the availability of cadmium, zinc and nickel can, moreover, be effectively reduced and the concentrations of these elements in the plants lowered. The conclusion from this study is that moderate recycling of low-heavy-metal sewage sludge in farming will be safe as long as, especially, cadmium concentrations are critically monitored and as long as the soils in question are mainly medium to heavy ones with pH values higher than 6.5. (orig./Vhe) [de

  15. «THE HIDDEN PEDAGOGY» AS MEANS OF CULTURAL COMMUNICATION AND VALUABLE AND ECONOMIC SOCIALIZATION (ACCORDING TO MATERIALS OF THE VIRTUAL MUSEUM OF GERMANY

    Directory of Open Access Journals (Sweden)

    Olga Dmitrievna Fedotova

    2015-12-01

    Full Text Available In this article the problems of using the factors of the forming influence operating outside the standard educational environment in the open sociocultural practice are considered. The characteristic is given to the concept «the hidden pedagogy» which is introduced into scientific circulation abroad at two levels – as the conceptual metaphor and theoretical conceptualization fixing spheres of influence of informal education. It is shown that acquaintance with exhibits expands the idea of visitors of the economic, cultural and valuable orientations dominating in the past on the basis of the analysis of an exposition of the virtual museum of the bookmarks presented in Internet space by collectors from Germany. It is shown that the bookmark for pupils advertizing the examples of behavior directed on accumulation, saving is an indirect, but effective factor of the forming influence. The characteristic of activity of financial institutions and school savings banks on formation of economic installations of the adult and young population is given.

  16. Organizing and conducting career guidance and crime prevention among young people by means of physical culture and sports specialized university departments Internal Affairs of Ukraine.

    Directory of Open Access Journals (Sweden)

    Zakorko I.P

    2011-06-01

    Full Text Available At the present stage of social development takes on special urgency the problem of finding new means and methods of crime prevention among youth in the context of strengthening the nation's health. In our opinion, employees of the Ukrainian Interior Ministry must take a decisive role in solving these problems. One of the most effective ways to solve them is to organize youth sports schools and clubs to professionally-applied sports at the bases of relevant departments of higher educational institutions of Ministry of Internal Affairs of Ukraine, the involvement of the best specialists and trainers.

  17. Co-culture of neural crest stem cells (NCSC and insulin producing beta-TC6 cells results in cadherin junctions and protection against cytokine-induced beta-cell death.

    Directory of Open Access Journals (Sweden)

    Anongnad Ngamjariyawat

    Full Text Available PURPOSE: Transplantation of pancreatic islets to Type 1 diabetes patients is hampered by inflammatory reactions at the transplantation site leading to dysfunction and death of insulin producing beta-cells. Recently we have shown that co-transplantation of neural crest stem cells (NCSCs together with the islet cells improves transplantation outcome. The aim of the present investigation was to describe in vitro interactions between NCSCs and insulin producing beta-TC6 cells that may mediate protection against cytokine-induced beta-cell death. PROCEDURES: Beta-TC6 and NCSC cells were cultured either alone or together, and either with or without cell culture inserts. The cultures were then exposed to the pro-inflammatory cytokines IL-1β and IFN-γ for 48 hours followed by analysis of cell death rates (flow cytometry, nitrite production (Griess reagent, protein localization (immunofluorescence and protein phosphorylation (flow cytometry. RESULTS: We observed that beta-TC6 cells co-cultured with NCSCs were protected against cytokine-induced cell death, but not when separated by cell culture inserts. This occurred in parallel with (i augmented production of nitrite from beta-TC6 cells, indicating that increased cell survival allows a sustained production of nitric oxide; (ii NCSC-derived laminin production; (iii decreased phospho-FAK staining in beta-TC6 cell focal adhesions, and (iv decreased beta-TC6 cell phosphorylation of ERK(T202/Y204, FAK(Y397 and FAK(Y576. Furthermore, co-culture also resulted in cadherin and beta-catenin accumulations at the NCSC/beta-TC6 cell junctions. Finally, the gap junction inhibitor carbenoxolone did not affect cytokine-induced beta-cell death during co-culture with NCSCs. CONCLUSION: In summary, direct contacts, but not soluble factors, promote improved beta-TC6 viability when co-cultured with NCSCs. We hypothesize that cadherin junctions between NCSC and beta-TC6 cells promote powerful signals that maintain beta

  18. Stool Culture

    Science.gov (United States)

    ... infections and may be identified with a stool culture. Some important examples include: Escherichia coli 0157:H7 and other toxin- ... the toxin-producing C. difficile will be performed. Examples of other less common causes include: ... of stool cultures that are reported as negative usually reflect the ...

  19. Agency, Context and Meaning

    DEFF Research Database (Denmark)

    Folkmann, Mads Nygaard

    2014-01-01

    The paper is a meta-discursive contribution to the discussion of how design can be understood as a medium of meaning formation and questioning of meaning. Further, the paper builds on plea for the role of humanities in relation to formulate relevant questions in design through conceptualizing the...... of meaning formulation and cultural contexts and, by this, contest design. In reflecting the foundational ground of design in terms of its agency, contexts and meaning constituents, design and its questioning of meaning can be critically reframed.......The paper is a meta-discursive contribution to the discussion of how design can be understood as a medium of meaning formation and questioning of meaning. Further, the paper builds on plea for the role of humanities in relation to formulate relevant questions in design through conceptualizing...... history, 2) the question of context in and of design, i.e. which contexts give meaning to design; this question calls for interpretive models of cultural analysis of the circuit of design in acknowledging phases and aspects of production, mediation and consumption, and 3) the question of the meaning...

  20. Meaning construction in remembering

    DEFF Research Database (Denmark)

    Wagoner, Brady

    2011-01-01

    F.C. Bartlett and L.S.Vygotsky were two seminal figures in the psychological study of remembering. Both emphasized the role of meaning and imagination in this process. Bartlett did this by showing the systematic and holistic changes that ensue when cultural material is repeatedly reproduced outsi...

  1. O processo de cuidar da pessoa que sofreu queimaduras: significado cultural atribuído por familiares El proceso de cuidar de personas que sufrieron quemadura: significado cultural atribuido por familiares The process of delivering care to a burned person: cultural meaning attributed by relatives

    Directory of Open Access Journals (Sweden)

    Lídia Aparecida Rossi

    2001-12-01

    Full Text Available Os objetivos deste estudo etnográfico foram identificar o significado cultural atribuído por familiares à participação no processo de cuidar da pessoa ultima de queimadura; identificar as estratégias de cuidado que os familiares focalizam frente à participação no cuidado. Os dados foram coletados mediante entrevistas semi-estruturadas com 25 familiares de clientes que sofreram queimaduras, admitidos em uma Unidade de Queimados e observações participantes. Os resultados mostram que o significado cultural atribuído por familiares de pessoas vítimas queimaduras ao processo de cuidar tem duas dimensões: a física e a moral e gera principalmente medo.Los objetivos fueron investigar los significados culturales atribuidos por familiares a la participación en el proceso de cuidar de personas víctimas de quemadura; identificar las estratégias que los familiares enfocan en su participación en el cuidado. Los datos fueron colectados a través de entrevistas semi-estructuradas con 25 familiares de clientes quemados admitidos en una Unidad de Quemados y a través de observaciones participantes realizadas durante el período de visitas y en reuniones con familiares de clientes. Los resultados de este estudio muestran que la perspectiva de cuidar de una persona que sufrió una quemadura tiene dos dimensiones: la física y la moral, y genera principalmente miedo.This study aimed at investigating the cultural meanings attributed by relatives to their participation in the process of delivering care to burned people; identifying the care strategies focused on by relatives during their participation in the care process. Data were collected by means of semi-structured interviews with 25 relatives of burned patients admitted in a Unit for Burned and by participant observation. The results of this study show that the perspective of giving care to a burned person has two dimensions: a physical dimension and a moral one; and generates mainly fear.

  2. PRESERVATIVE POTENTIAL OF PURIFIED BACTERIOCIN PRODUCED FROM BREVIBACILLUS BORSTELENSIS AG1 ISOLATED FROM MARCHA – A TRADITIONAL WINE STARTER CULTURE CAKE IN TOMATO PASTE

    OpenAIRE

    Anupama Gupta; Nivedita Sharma; Neha Gautam

    2015-01-01

    Purified bacteriocin produced from Brevibacillus borstelensis AG1 isolated from Marcha a local wine starter herbal cake, was used to enhance the shelf life of tomato paste. Preservative effect of purified bacteriocin was studied for nine days in tomato paste inoculated with food borne pathogens and was compared to commercial biopreservative – nisin and chemical preservative – sodium benzoate. The indicator strains i.e. Listeria monocytogenes MTCC839, Bacillus subtilis CRI and Clostridium perf...

  3. Testing for a cultural influence on reading for meaning in the developing brain: the neural basis of semantic processing in Chinese children

    Directory of Open Access Journals (Sweden)

    Tai-Li Chou

    2009-11-01

    Full Text Available Functional magnetic resonance imaging (fMRI was used to explore the neural correlates of semantic judgments in a group of 8- to 15-year-old Chinese children. Participants were asked to indicate if pairs of Chinese characters presented visually were related in meaning. The related pairs were arranged in a continuous variable according to association strength. Pairs of characters with weaker semantic association elicited greater activation in the mid ventral region (BA 45 of left inferior frontal gyrus, suggesting increased demands on the process of selecting appropriate semantic features. By contrast, characters with stronger semantic association elicited greater activation in left inferior parietal lobule (BA 39, suggesting stronger integration of highly related features. In addition, there was a developmental increase, similar to previously reported findings in English, in left posterior middle temporal gyrus (BA 21, suggesting that older children have more elaborated semantic representations. There were additional age-related increases in the posterior region of left inferior parietal lobule and in the ventral regions of left inferior frontal gyrus, suggesting that reading acquisition relies more on the mapping from orthography to semantics in Chinese children as compared to previously reported findings in English.

  4. Isolation of TDA-producing Phaeobacter strains from sea bass larval rearing units and their probiotic effect against pathogenic Vibrio spp. in Artemia cultures

    DEFF Research Database (Denmark)

    Grotkjær, Torben; Bentzon-Tilia, Mikkel; D'Alvise, Paul

    2016-01-01

    V. harveyi, which is the major bacterial pathogen in crustaceans and Mediterranean sea bass larvae cultures. Concomitantly, they significantly improved survival of V. harveyi-infected brine shrimp. 16S rRNA gene sequence homology identified the antagonists as Phaeobacter sp., and in silico DNA...... Vibrio anguillarum and reduce mortality in V. anguillarum-infected cod and turbot larvae. In this study, it was demonstrated that antagonistic Roseobacter-clade bacteria could be isolated from sea bass larval rearing units. In addition, it was shown that they not only antagonized V. anguillarum but also......-producing Phaeobacter isolated from Mediterranean marine larviculture are promising probiotic bacteria against pathogenic Vibrio in crustacean live-feed cultures for marine fish larvae....

  5. How culture affects management?

    OpenAIRE

    Billi, Lorena

    2012-01-01

    The study is about how culture affects management. Culture can have many different meanings. Management has also many different ways to be approached. While doing research about cultures, the study will try to analyze how the culture affects the management. The study starts with a full explanation of the meaning of culture. Some previous analysis and studies are added to illustrate my study on the subject. The effect culture has on management is studied at different levels. The study does not...

  6. Evaluation of genotoxic effect of prozac (fluoxetine without and with addition of vitamins A and C by means of the comet assay in culture of CHO-K1 cells

    Directory of Open Access Journals (Sweden)

    Noélle Giacomini Lemos

    2005-12-01

    Full Text Available The fluoxetine, commercially named Prozac, is efficient against depression and anxiety, with lower risk of collateral effects. However, the possible genotoxic effects are still unknown. The use of vitamins as protectors against damages on cells and DNA has been evaluated, mainly for vitamins A and C. Furthermore, the associative effect of vitamins with several medicines demands studies. The evaluations of genotoxic effect of Prozac and vitamins A and C protective effect were carried out in culture of Chinese hamster ovary cells, CHO-K1, by means of the comet test. The Prozac was used, in liquid formulation, diluted in 5µg, 1µg and 0.2 µg/mL of culture medium. The vitamins were used, in liquid formulation, at the concentrations of 3µg and 880,5 µg/mL of culture medium to vitamins A and C, respectively. The treatments were carried out during 1 hour. The obtained data demonstrated that only the highest concentration of Prozac (5 µg is genotoxic and both vitamins A and C reduced such genotoxicity. The data suggest a follow-up on patients who use Prozac and the possibility of vitamins A and C association in order to minimize the collateral genotoxic effects.

  7. Polysaccharide-producing microalgae

    Energy Technology Data Exchange (ETDEWEB)

    Braud, J.P.; Chaumont, D.; Gudin, C.; Thepenier, C.; Chassin, P.; Lemaire, C.

    1982-11-01

    The production of extracellular polysaccharides is studied with Nostoc sp (cyanophycus), Porphiridium cruentum, Rhodosorus marinus, Rhodella maculata (rhodophyci) and Chlamydomonas mexicana (chlorophycus). The polysaccharides produced are separated by centrifugation of the culture then precipitation with alcohol. Their chemical structure was studied by infrared spectrometry and acid hydrolysis. By their rheological properties and especially their insensitivity to temperatrure and pH variations the polysaccharides produced by Porphryridium cruentum and Rhodella maculata appear as suitable candidates for industrial applications.

  8. Some major deviations for biomass determination by indirect method and estimation based on alkali consumption. [Ratio of cell mass produced and alkali consumed; diesel fuel culture medium

    Energy Technology Data Exchange (ETDEWEB)

    Concone, B R.V.; Doin, P A; Pinto, A G

    1978-01-01

    Some factors like the variation of the liquid volume, the variation of cellular nitrogen content and the mass of cells taken with the samples during batch cultivation of microorganisms on diesel oil, were considered for the computation of the ratio between cell mass produced and the mass of alkali consumed to maintain constant the pH of the fermentation medium. The results obtained showed that if such ratios are computed with cell concentration instead of cell mass the deviations can be of the order of 27% caused by the variation of the liquid medium volume. Otherwise, the results showed also that those ratios are variable during batch cultivation on diesel oil probably because of the variations on the nitrogen content of microorganisms. The relative difference between the mass of cells measured and the mass of cells calculated from the alkali consumption curve can be of the order of 63%.

  9. PRESERVATIVE POTENTIAL OF PURIFIED BACTERIOCIN PRODUCED FROM BREVIBACILLUS BORSTELENSIS AG1 ISOLATED FROM MARCHA – A TRADITIONAL WINE STARTER CULTURE CAKE IN TOMATO PASTE

    Directory of Open Access Journals (Sweden)

    Anupama Gupta

    2015-04-01

    Full Text Available Purified bacteriocin produced from Brevibacillus borstelensis AG1 isolated from Marcha a local wine starter herbal cake, was used to enhance the shelf life of tomato paste. Preservative effect of purified bacteriocin was studied for nine days in tomato paste inoculated with food borne pathogens and was compared to commercial biopreservative – nisin and chemical preservative – sodium benzoate. The indicator strains i.e. Listeria monocytogenes MTCC839, Bacillus subtilis CRI and Clostridium perfringens MTCC1739 were used at the amount 8.16, 8.13 and 8.18 log CFU/ml. Viable cells were counted periodically and a consistent reduction in number of viable cells of each tested pathogen was observed. It was found antagonistic against L. monocytogenes MTCC839, B. subtilis CRI and C. perfringes MTCC1739 which are the most challengeable and food borne pathogens found in processed vegetables products. Purified bacteriocin was found active over a wide pH range i.e. 3.0 to 11.0 and was able to withstand temperature up to 100oC. It showed a better preservative potential by reducing pathogenic load of the tested strains (by 2.02, 2.05 and 2.02 log cycles (CFU/ml of L. monocytogenes MTCC839, B. subtilis CRI and C. perfringes MTCC1739, respectively in tomato paste as compared to control (without bacteriocin. This proves efficiency of bacteriocin produced by B. borstelensis AG1 as biopreservative to enhance the safety and shelf life of acidic foods.

  10. Significados e sentidos da música: uma breve "composição" a partir da psicologia histórico-cultural Significados y sentidos de la música: una breve "composición" a partir de la psicología histórico-cultural Meanings and senses of music: a brief composition from cultural-historical psychology

    Directory of Open Access Journals (Sweden)

    Patrícia Wazlawick

    2007-04-01

    Full Text Available Este artigo discute a construção dos significados e sentidos da música a partir da Psicologia Histórico-Cultural, principalmente com a contribuição de L. S. Vygotski, buscando tecer uma interface com as idéias dos musicoterapeutas noruegueses Even Ruud e Brynjulf Stige, com base na filosofia de Ludwig Wittgenstein. Quando se vivencia a música, não se estabelece relação apenas com a matéria musical em si, mas com toda uma rede de significados construídos no mundo social, em contextos coletivos mais amplos e em contextos singulares. Dessa forma, os significados e sentidos da música são construídos a partir do contexto social, econômico, político, de vivências concretas e da "utilização viva" da música por sujeitos em relação, onde articulam sua dimensão afetiva, desejos e motivações.Este artículo discute la construcción de los significados y sentidos de la música a partir de la Psicología Histórico Cultural, principalmente con la contribución de L. S. Vygotski, buscando formar una conexión con las ideas de los musicoterapeutas noruegos Even Ruud y Brynjulf Stige, en base a la filosofía de Ludwig Wittgenstein. Cuando se vivencia la música, no sólo se relaciona con la materia musical en sí, sino con toda una red de significados construidos en el mundo social, en contextos colectivos más amplios y en contextos singulares. De esa forma, los significados y sentidos de la música son construidos a partir del contexto social, económico, político, de vivencias concretas y de la "utilización viva" de la música por sujetos interrelacionados, donde articulan su dimensión afectiva, deseos y motivaciones.The construction of meanings and senses in music from the point of view of Cultural-Historical Psychology, with special reference to L. S. VygotskI, is discussed. An interface with the ideas of Norwegian music-therapists Even Ruud and Brynjulf Stige, grounded on Ludwig Wittgenstein's philosophy, is highlighted. The

  11. Cultural ergonomics in interactional and experiential design: conceptual framework and case study of the Taiwanese twin cup.

    Science.gov (United States)

    Lin, Chih-Long; Chen, Si-Jing; Hsiao, Wen-Hsin; Lin, Rungtai

    2016-01-01

    Cultural ergonomics is an approach that considers interaction- and experience-based variations among cultures. Designers need to develop a better understanding of cultural ergonomics not just to participate in cultural contexts but also to develop interactive experiences for users. Cultural ergonomics extends our understanding of cultural meaning and our ability to utilize such understanding for design and evaluate everyday products. This study aims to combine cultural ergonomics and interactive design to explore human-culture interaction in user experiences. The linnak is a typical Taiwanese aboriginal cultural object. This study examined the cultural meaning and operational interface of the linnak, as well as the scenarios in which it is used in interaction and user experiences. The results produced a cultural ergonomics interface for examining the manner in which designers communicate across cultures as well as the interweaving of design and culture in the design process. Copyright © 2015 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  12. AFLP and AMP Fingerprints as Markers to Evaluate Genetic Differences between Medicago truncatula Line Jemalong and 2HA, a New Line Produced by in vitro Culture Selection

    Directory of Open Access Journals (Sweden)

    R.R. Irwanto

    2008-09-01

    Full Text Available A new line, Medicago truncatula cv. Jemalong 2HA (herein known as 2HA has been developed via repetitive regeneration and selection of M. truncatula cv. Jemalong. During somatic embryogenesis, 2HA produces 500 times more embryos than its progenitor, Jemalong. It is interesting to study if those two lines are isogenic or has genetic differences. The main objectives of the study was to evaluate the genotypic differences between Jemalong and 2HA also to evaluate the methylation event in 2HA utilized two DNA fingerprinting techniques, i.e AFLP fingerprints (Amplified Length of Polymorphism and AMP (Amplified Methylation Polymorphism. The results showed that AFLP analysis using eight primers combinations could not detect any differences between Jemalong and 2HA. However, using AMP methylation sensitive primers it could detect 15 polymorphisms out of 840 markers. These results lead to a conclusion that Jemalong and 2HA are isogenic lines. 2HA may have higher regeneration capacities due to methylation process which occurs during the production of 2HA through repetitive regeneration cycles.

  13. Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases.

    Science.gov (United States)

    Franco Marcelino, Paulo Ricardo; da Silva, Vinícius Luiz; Rodrigues Philippini, Rafael; Von Zuben, Cláudio José; Contiero, Jonas; Dos Santos, Júlio César; da Silva, Silvio Silvério

    2017-01-01

    Biosurfactants are microbial metabolites with possible applications in various industrial sectors that are considered ecofriendly molecules. In recent years, some studies identified these compounds as alternatives for the elimination of vectors of tropical diseases, such as Aedes aegypti. The major bottlenecks of biosurfactant industrial production have been the use of conventional raw materials that increase production costs as well as opportunistic or pathogenic bacteria, which restrict the application of these biomolecules. The present study shows the potential of hemicellulosic sugarcane bagasse hydrolysate as a raw material for the production of a crystalline glycolipidic BS by Scheffersomyces stipitis NRRL Y-7124, which resulted in an emulsifying index (EI24) of 70 ± 3.4% and a superficial tension of 52 ± 2.9 mN.m-1. Additionally, a possible new application of these compounds as biolarvicides, mainly against A. aegypti, was evaluated. At a concentration of 800 mg.L-1, the produced biosurfactant caused destruction to the larval exoskeletons 12 h after application and presented an letal concentration (LC50) of 660 mg.L-1. Thus, a new alternative for biosurfactant production using vegetal biomass as raw material within the concept of biorefineries was proposed, and the potential of the crystalline glycolipidic biosurfactant in larvicidal formulations against neglected tropical disease vectors was demonstrated.

  14. Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases.

    Directory of Open Access Journals (Sweden)

    Paulo Ricardo Franco Marcelino

    Full Text Available Biosurfactants are microbial metabolites with possible applications in various industrial sectors that are considered ecofriendly molecules. In recent years, some studies identified these compounds as alternatives for the elimination of vectors of tropical diseases, such as Aedes aegypti. The major bottlenecks of biosurfactant industrial production have been the use of conventional raw materials that increase production costs as well as opportunistic or pathogenic bacteria, which restrict the application of these biomolecules. The present study shows the potential of hemicellulosic sugarcane bagasse hydrolysate as a raw material for the production of a crystalline glycolipidic BS by Scheffersomyces stipitis NRRL Y-7124, which resulted in an emulsifying index (EI24 of 70 ± 3.4% and a superficial tension of 52 ± 2.9 mN.m-1. Additionally, a possible new application of these compounds as biolarvicides, mainly against A. aegypti, was evaluated. At a concentration of 800 mg.L-1, the produced biosurfactant caused destruction to the larval exoskeletons 12 h after application and presented an letal concentration (LC50 of 660 mg.L-1. Thus, a new alternative for biosurfactant production using vegetal biomass as raw material within the concept of biorefineries was proposed, and the potential of the crystalline glycolipidic biosurfactant in larvicidal formulations against neglected tropical disease vectors was demonstrated.

  15. Significados culturales del concepto de salud en el trabajo en profesionales en formación Cultural meanings of the concept of health at work in professionals in training

    Directory of Open Access Journals (Sweden)

    María de los Ángeles Aguilera Velasco

    2012-09-01

    Full Text Available Introducción: El concepto de salud en el trabajo implica una serie de significados para los profesionales de la salud ocupacional, quienes los utilizan para orientar, interactuar, discutir, definir, categorizar e interpretar sus prácticas. Conocer dichos significados es relevan te porque permite aportar datos útiles para el diseño de programas educativos tendientes a la prevención de accidentes y enfermedades laborales. Objetivo: Explorar los significados culturales del concepto de salud en el trabajo, antes y después de un diplomado con duración de seis meses, en profesionales de la salud ocupacional. Metodología: Estudio cualitativo con diseño descriptivo exploratorio. Realizado en Guadalajara, México de febrero a julio de 2011. Los datos fueron recolectados con la técnica de listados libres y analizados a través del modelo de dominios culturales. Resultados: El concepto de salud en el trabajo, antes del diplomado se definió a través de los términos riesgo y seguridad. Después del diplomado, la salud en el trabajo se definió a través de los elementos seguridad y prevención. Conclusiones: La cultura de la prevención se logra a través de un proceso de aprendizaje que incluye conocimientos sobre los riesgos laborales y la normatividad vigente.Introduction: The concept of health in the workplace involves a number of meanings for occupational health professionals, who are used to guide, interact, discuss, define, categorize, and interpret their practices. Knowing these meanings is relevate because it allows to provide useful data for the design of educational programmes aimed at the prevention of accidents and occupational diseases. Objective: Explore the cultural meanings of the concept of health at work, before and after the training with duration of six months, in occupational health professionals. Methodology: Qualitative study with exploratory descriptive design, in Guadalajara, Mexico from February to July 2011. The data

  16. The influence of metal speciation in combustion waste on the efficiency of Cu, Pb, Zn, Cd, Ni and Cr bioleaching in a mixed culture of sulfur-oxidizing and biosurfactant-producing bacteria.

    Science.gov (United States)

    Karwowska, Ewa; Wojtkowska, Małgorzata; Andrzejewska, Dorota

    2015-12-15

    Metal leachability from ash and combustion slag is related to the physico-chemical properties, including their speciation in the waste. Metals speciation is an important factor that influences the efficiency of metal bioleaching from combustion wastes in a mixed culture of acidophilic and biosurfactant-producing bacteria. It was observed that individual metals tended to occur in different fractions, which reflects their susceptibility to bioleaching. Cr and Ni were readily removed from wastes when present with a high fraction bound to carbonates. Cd and Pb where not effectively bioleached when present in high amounts in a fraction bound to organic matter. The best bioleaching results were obtained for power plant slag, which had a high metal content in the exchangeable, bound to carbonates and bound to Fe and Mg oxides fractions- the metal recovery percentage for Zn, Cu and Ni from this waste exceeded 90%. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Meaning Structures in a Contested Issue Field

    DEFF Research Database (Denmark)

    Meyer, Renate; Höllerer, Markus

    2010-01-01

    When organizational concepts spread beyond national and cultural boundaries, they must pass through powerful filters of local cultural and structural opportunities and constraints in order to mobilize legitimacy. Struggles over their meaning are intensified if they challenge the prevailing order...

  18. The visible evidence of cultural producers

    Directory of Open Access Journals (Sweden)

    Maureen Mahon

    2008-01-01

    Full Text Available El texto revisa las investigaciones antropológicas recientes que se han centrado en el estudio de las prácticas a través de las cuales los individuos y los grupos humanos producen música, videos, películas, artes visuales y teatro, tomando en cuenta los marcos ideológicos e institucionales dentro de los cuales ocurre esta producción Estas formas mediáticas y de cultura popular son presentadas como campos dentro de los cuales los actores sociales discuten acerca de los significados, y como evidencia observable de procesos y relaciones sociales. Desde este punto de vista, Mahon muestra cómo las distintas investigaciones antropológicas han abordado las dimensiones sociales, políticas y estéticas de estos productos culturales.

  19. Study of the behaviour of P-oxides produced in the combustion of phosphorus-bearing organic compounds and their absorption on suitable substances by means of compounds labelled with 32P. I

    International Nuclear Information System (INIS)

    Binkowski, J.; Gizinski, S.; Kaminski, R.; Reimschuessel, W.

    1976-01-01

    During the pyrolytic reaction of phosphorus-bearing compounds in an atmosphere of oxygen, there are produced P-oxides, which interfere in the determination of carbon and hydrogen. The behaviour of these P-oxides was studied intensively in the empty combustion tube in dependence on temperature, velocity of the carrier gas, the nature of the combustion and the positioning of the tube. The P-oxides were determined by the employment of 32 P-labelled aniline phosphate and by activation measurement of the 32 P directly through the tube walls as well as in the finely divided material. In conventional combustions the P-oxides separate abundantly on the walls of the tube provided that the gas velocity is low, especially in those zones of the tube where there is marked temperature lowering, or where very high temperature prevail. Deposited P-oxides are swept out of the tube only in the course of a very long time. Consequently a tube in which the P-bearing compounds have been burned will interfere because of the eluted P-oxides and hence will interfere also if P-free compounds are burned in this same tube

  20. Quantitative and qualitative research across cultures and languages: cultural metrics and their application.

    Science.gov (United States)

    Wagner, Wolfgang; Hansen, Karolina; Kronberger, Nicole

    2014-12-01

    Growing globalisation of the world draws attention to cultural differences between people from different countries or from different cultures within the countries. Notwithstanding the diversity of people's worldviews, current cross-cultural research still faces the challenge of how to avoid ethnocentrism; comparing Western-driven phenomena with like variables across countries without checking their conceptual equivalence clearly is highly problematic. In the present article we argue that simple comparison of measurements (in the quantitative domain) or of semantic interpretations (in the qualitative domain) across cultures easily leads to inadequate results. Questionnaire items or text produced in interviews or via open-ended questions have culturally laden meanings and cannot be mapped onto the same semantic metric. We call the culture-specific space and relationship between variables or meanings a 'cultural metric', that is a set of notions that are inter-related and that mutually specify each other's meaning. We illustrate the problems and their possible solutions with examples from quantitative and qualitative research. The suggested methods allow to respect the semantic space of notions in cultures and language groups and the resulting similarities or differences between cultures can be better understood and interpreted.

  1. Significados e sentidos de saúde socializados por artefatos culturais: leituras das imagens de advertência nos maços de cigarro Senses and meanings of health socialized by cultural devices: readings of the warning images on cigarettes packs

    Directory of Open Access Journals (Sweden)

    Rogério Dias Renovato

    2009-10-01

    possibilities of readings that can be present at these devices.We understand the propagated images as cultural pedagogies that behave as texts to be read, building up representations that can be assumed as true effect, and attributes to the citizen the necessity of constant control of its action. Reassuring the biomedicine and the health institutions, an onedimensional vision is propagated disregarding the complexity of this problem. We understand that the cultural pedagogies are part of competition territories, where the directions and meanings can be re-prepared producing hybrid identities that build up its own identity origins over this tangle of power relations.

  2. The Politics of Culture

    Directory of Open Access Journals (Sweden)

    John Storey

    2017-01-01

    Full Text Available This article provides an overview over the evolution of thinking about "culture" in the work of Raymond Williams. With the introduction of Antonio Gramsci's concept of hegemony culture came to be understood as consisting of not only shared, but contested meanings as well. On the basis of this redefinition by Williams, cultural studies was able to delineate culture as the production, circulation, and consumption of meanings that become embodied and embedded in social practice.

  3. Crescimento do fungo simbionte de formigas cortadeiras do gênero Acromyrmex em meios de cultura com diferentes extratos Growth of symbiont fungi of cutter ants of the genus Acromyrmex in means of culture with different extracts

    Directory of Open Access Journals (Sweden)

    Regina da Silva Borba

    2006-06-01

    plants have been accomplished to evaluate the effect on the development of the mushroom symbiont. The objective of this work was to evaluate the influence of added culture means of different extracts on the growth of the L. gongylophorus mushroom. Where mushrooms of the species Acromyrmex ambiguus, Acromyrmex crassispinus, Acromyrmex heyeri and Acromyrmex lundi were inoculated in the means Nutritious Ágar and BDA, both added of molasses and extracts of Lolium multiflorum Lam., Typha angustifolia L. and ant (Atta sexdens piriventris. The Pagnocca medium was used as pattern. The plates were maintained in cameras acclimatized to the temperature of 25 + 1°C and 24 hours of darkness, for a period of 49 days. The fungal growth was evaluated with base in the diameter of the colony, which was accomplished with weekly intervals, totalizing seven evaluations. Also the dry weight was evaluated from the mushroom to the 53 days. The results showed that the mushroom cultivated by different species of ants has differentiated growth in culture means with different extracts.

  4. KEEPING CULTURAL GENES ALIVE

    Institute of Scientific and Technical Information of China (English)

    Bai Shi

    2012-01-01

    China's contemporary culture and the protection of its diverse cultural heritage have become some of the most talked about issues today.Cultural prosperity was put forward as an important objective of the Central Government's national development strategy last year.However,the industrialization and commercialization of China's culture have been both criticized and celebrated.Many scholars believe industrialization and enormous government investment may not be the best means to protect intangible cultural heritage (ICH).

  5. Producing cement

    Energy Technology Data Exchange (ETDEWEB)

    Stone, E G

    1923-09-12

    A process and apparatus are described for producing Portland cement in which pulverized shale is successively heated in a series of inclined rotary retorts having internal stirrers and oil gas outlets, which are connected to condensers. The partially treated shale is removed from the lowermost retort by a conveyor, then fed separately or conjointly into pipes and thence into a number of vertically disposed retorts. Each of these retorts may be fitted interiorly with vertical arranged conveyors which elevate the shale and discharge it over a lip, from whence it falls to the bottom of the retorts. The lower end of each casing is furnished with an adjustable discharge door through which the spent shale is fed to a hopper, thence into separate trucks. The oil gases generated in the retorts are exhausted through pipes to condensers. The spent shale is conveyed to a bin and mixed while hot with ground limestone. The admixed materials are then ground and fed to a rotary kiln which is fired by the incondensible gases derived from the oil gases obtained in the previous retorting of the shale. The calcined materials are then delivered from the rotary kiln to rotary coolers. The waste gases from the kiln are utilized for heating the retorts in which the ground shale is heated for the purpose of extracting therefrom the contained hydrocarbon oils and gases.

  6. The Curing Agent Sodium Nitrite, Used in the Production of Fermented Sausages, Is Less Inhibiting to the Bacteriocin-Producing Meat Starter Culture Lactobacillus curvatus LTH 1174 under Anaerobic Conditions

    Science.gov (United States)

    Verluyten, Jurgen; Messens, Winy; De Vuyst, Luc

    2003-01-01

    Curvacin A is a listericidal bacteriocin produced by Lactobacillus curvatus LTH 1174, a strain isolated from fermented sausage. The response of this strain to an added curing agent (sodium nitrite) in terms of cell growth and bacteriocin production was investigated in vitro by laboratory fermentations with modified MRS broth. The strain was highly sensitive to nitrite; even a concentration of 10 ppm of curing agent inhibited its growth and both volumetric and specific bacteriocin production. A meat simulation medium containing 5 ppm of sodium nitrite was tested to investigate the influence of the gas phase on the growth and bacteriocin production of L. curvatus LTH 1174. Aerating the culture during growth had no effect on biomass formation, but the oxidative stress caused a higher level of specific bacteriocin production and led to a metabolic shift toward acetic acid production. Anaerobic conditions, on the other hand, led to an increased biomass concentration and less growth inhibition. Also, higher maximum volumetric bacteriocin activities and a higher level of specific bacteriocin production were obtained in the presence of sodium nitrite than in fermentations under aerobic conditions or standard conditions of air supply. These results indicate that the inhibitory effect of the curing agent is at least partially masked under anaerobic conditions. PMID:12839751

  7. The curing agent sodium nitrite, used in the production of fermented sausages, is less inhibiting to the bacteriocin-producing meat starter culture Lactobacillus curvatus LTH 1174 under anaerobic conditions.

    Science.gov (United States)

    Verluyten, Jurgen; Messens, Winy; De Vuyst, Luc

    2003-07-01

    Curvacin A is a listericidal bacteriocin produced by Lactobacillus curvatus LTH 1174, a strain isolated from fermented sausage. The response of this strain to an added curing agent (sodium nitrite) in terms of cell growth and bacteriocin production was investigated in vitro by laboratory fermentations with modified MRS broth. The strain was highly sensitive to nitrite; even a concentration of 10 ppm of curing agent inhibited its growth and both volumetric and specific bacteriocin production. A meat simulation medium containing 5 ppm of sodium nitrite was tested to investigate the influence of the gas phase on the growth and bacteriocin production of L. curvatus LTH 1174. Aerating the culture during growth had no effect on biomass formation, but the oxidative stress caused a higher level of specific bacteriocin production and led to a metabolic shift toward acetic acid production. Anaerobic conditions, on the other hand, led to an increased biomass concentration and less growth inhibition. Also, higher maximum volumetric bacteriocin activities and a higher level of specific bacteriocin production were obtained in the presence of sodium nitrite than in fermentations under aerobic conditions or standard conditions of air supply. These results indicate that the inhibitory effect of the curing agent is at least partially masked under anaerobic conditions.

  8. Aging in culture.

    Science.gov (United States)

    Fung, Helene H

    2013-06-01

    This article reviews the empirical studies that test socioemotional aging across cultures. The review focuses on comparisons between Western (mostly North Americans and Germans) and Eastern cultures (mostly Chinese) in areas including age-related personality, social relationships, and cognition. Based on the review, I argue that aging is a meaning-making process. Individuals from each cultural context internalize cultural values with age. These internalized cultural values become goals that guide adult development. When individuals from different cultures each pursue their own goals with age, cultural differences in socioemotional aging occur.

  9. Symbolic severance. Charlie or the broken circle of cultural mediation

    Directory of Open Access Journals (Sweden)

    Isabelle MATHIEU

    2015-07-01

    Full Text Available The cultural dimension of the attack on Charlie Hebdo raises questions about the meaning of the event in terms of public policies on art and culture. At issue particulary is the re-situation of those policies in their relationship with democratic achievement in the context of the French republican model. Analysis through the prism of cultural mediation, considered as a communication process designed ti produce a symbolic type of work, casts new light on the objectives and the ways and means of implementing such policies.

  10. Pierre Verger and the construction of african-brazilian’s cultural memory in O Cruzeiro: textual meanings through borders Pierre Verger e a construção da memória cultural afro-brasileira em O Cruzeiro: sentidos textuais através das fronteiras

    Directory of Open Access Journals (Sweden)

    Beatriz Marocco

    2010-03-01

    Full Text Available Based on the concepts of border, culture and memory postulated by Yuri Lotman, this paper aims to analyze Pierre Verger photos in the article titled “Capoeira mata um!”, published on O Cruzeiro magazine. The purpose is to understand the connection of meanings on semiotic boundaries mechanisms in relation to Afro Brazilian culture and memory text constructions in order to contribute to media communication studies. A partir dos conceitos de fronteira, cultura e memória postulados por Yuri Lotman, o objetivo deste artigo é analisar as fotografias de Pierre Verger na matéria “Capoeira mata um!” da revista O Cruzeiro (1948, no intuito de entender a articulação de sentidos nos mecanismos de fronteiras semióticas para a construção de textos da cultura e da memória afrobrasileira, e contribuir para os estudos da Comunicação midiática.

  11. Translation and Culture:Translation as a Cross-cultural Mediation

    Institute of Scientific and Technical Information of China (English)

    叶谋锦

    2013-01-01

    Translation is a complex activity which involves language competence as well as proficiency in multiculturalism. From the perspective of multiculturalism, translation resembles recreation of source text by grasping essential meanings to produce a sub-tle target text which can be clearly perceived by target readers. Ignoring cultural issues can present serious mistranslations in the field of advertising translation. This paper aims to explore the significance of connotation confined by the framework of culture and point out that verbal translation is a dangerous inclination by illustrating three business examples. This paper argues that cross-cultural mediation plays an important role in translation.

  12. Cultural Analysis - towards cross-cultural understanding

    DEFF Research Database (Denmark)

    Gullestrup, Hans

    The book considers intercultural understanding and co-action, partly by means of general insight into concept of culture and the dimensions which bring about cultural differences, and partly as a methodology to analyse a certain culture - whether one's own or others'. This leads towards an unders......The book considers intercultural understanding and co-action, partly by means of general insight into concept of culture and the dimensions which bring about cultural differences, and partly as a methodology to analyse a certain culture - whether one's own or others'. This leads towards...... a theoretical/abstract proposal for cultural understanding. The second part presents a theoretical/abstract proposal for under-standing intercultural plurality and complexity. The third part provides an empirical model for the analysis of intercultural co-action. Finally, the fourth part present and discusses...

  13. Isolation and molecular characterization of phytase producing ...

    African Journals Online (AJOL)

    Isolation and molecular characterization of phytase producing bacteria from Malaysia hot springs. ... The strains were further analyzed in broth culture using sodium phytate as substrate. Among them, strain L3 was selected as the best producer (0.16 U/ml after 72 h of culture). This phytase showed optimal activity at 37 °C ...

  14. Music, memory and meaning

    OpenAIRE

    Fair, Laura

    2012-01-01

    I his paper examines the music and career of Siti binti Saadi, a famous taarab musician who performed in Zanzibar during the 1920s and 1930s. Relying on four distinctive types of evidence: her recorded music, written documentation produced in East Africa, interviews with men and women who heard her perform and records of company executives I compare perspectives regarding the source of power and authority attributed to her voice as well as the meaning of her music. Siti binti Saadi was the fi...

  15. Ordinary and extraordinary means.

    Science.gov (United States)

    Gillon, R

    1986-01-25

    The Roman Catholic doctrine of ordinary and extraordinary means in patient care decisions is the subject of this essay in Gillon's series on medical ethics. He briefly traces the Church history of this doctrine, which holds that saving life is not obligatory if doing so would be excessively burdensome or disproportionate in relation to the expected benefits. The burdens and benefits are to be weighed in the context of "circumstances of persons, places, times, and cultures," and factors such as the costs and risks of undergoing a proposed treatment may be considered. Gillon also notes the disagreement among Roman Catholic commentators over whether it is ever permissible to discontinue feeding as a burdensome, extraordinary treatment. He concludes that, despite different weightings of harms and benefits, Roman Catholic and non-Catholic thinkers are in accord over the appropriate moral approach to deciding when treatment is not obligatory.

  16. Bourlingue culturelle (Cultural Rambling).

    Science.gov (United States)

    Thieriot, Jacques

    1980-01-01

    Reflects on the meaning of cultural education and activity in France as inspired by the need to establish a new form of cultural dialogue between France and the people of other countries. Problems related to financing cultural activities, reaching various types of audience, and methodology are discussed. (AMH)

  17. Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis

    Science.gov (United States)

    Roschanski, Nicole; Fischer, Jennie; Falgenhauer, Linda; Pietsch, Michael; Guenther, Sebastian; Kreienbrock, Lothar; Chakraborty, Trinad; Pfeifer, Yvonne; Guerra, Beatriz; Roesler, Uwe H.

    2018-01-01

    Carbapenems are last-resort antibiotics used in human medicine. The increased detection of carbapenem-resistant Enterobacteriaceae (CRE) is therefore worrying. In 2011 we reported the first livestock-associated VIM-1-producing Salmonella (S.) enterica serovar Infantis (R3) isolate from dust, sampled in a German chicken fattening farm. Due to this observation we retrospectively investigated more than 536 stored bacterial cultures, isolated from 45 chicken fattening farms during the years 2011 and 2012. After a non-selective overnight incubation, the bacteria were transferred to selective media. Escherichia (E.) coli and Salmonella growing on these media were further investigated, including antibiotic susceptibility testing, carbapenemase gene screening and whole genome sequencing (WGS). In total, four CRE were found in three out of 45 investigated farms: Besides R3, one additional Salmonella (G-336-1a) as well as two E. coli isolates (G-336-2, G-268-2). All but G-268-2 harbored the blaVIM-1 gene. Salmonella isolates R3 and G-336-1 were closely related although derived from two different farms. All three blaVIM-1-encoding isolates possessed identical plasmids and the blaVIM-1- containing transposon showed mobility at least in vitro. In isolate G-268-2, the AmpC beta-lactamase gene blaCMY-2 but no known carbapenemase gene was identified. However, a transfer of the phenotypic resistance was possible. Furthermore, G-268-2 contained the mcr-1 gene, combining phenotypical carbapenem- as well as colistin resistance in one isolate. Carbapenem-resistant Enterobacteriaceae have been found in three out of 45 investigated chicken flocks. This finding is alarming and emphasizes the importance of intervention strategies to contain the environmental spread of resistant bacteria in animals and humans. PMID:29636734

  18. Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011-2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis.

    Science.gov (United States)

    Roschanski, Nicole; Fischer, Jennie; Falgenhauer, Linda; Pietsch, Michael; Guenther, Sebastian; Kreienbrock, Lothar; Chakraborty, Trinad; Pfeifer, Yvonne; Guerra, Beatriz; Roesler, Uwe H

    2018-01-01

    Carbapenems are last-resort antibiotics used in human medicine. The increased detection of carbapenem-resistant Enterobacteriaceae (CRE) is therefore worrying. In 2011 we reported the first livestock-associated VIM-1-producing Salmonella ( S .) enterica serovar Infantis (R3) isolate from dust, sampled in a German chicken fattening farm. Due to this observation we retrospectively investigated more than 536 stored bacterial cultures, isolated from 45 chicken fattening farms during the years 2011 and 2012. After a non-selective overnight incubation, the bacteria were transferred to selective media. Escherichia (E.) coli and Salmonella growing on these media were further investigated, including antibiotic susceptibility testing, carbapenemase gene screening and whole genome sequencing (WGS). In total, four CRE were found in three out of 45 investigated farms: Besides R3, one additional Salmonella (G-336-1a) as well as two E. coli isolates (G-336-2, G-268-2). All but G-268-2 harbored the bla VIM-1 gene. Salmonella isolates R3 and G-336-1 were closely related although derived from two different farms. All three bla VIM-1 -encoding isolates possessed identical plasmids and the bla VIM-1- containing transposon showed mobility at least in vitro . In isolate G-268-2, the AmpC beta-lactamase gene bla CMY-2 but no known carbapenemase gene was identified. However, a transfer of the phenotypic resistance was possible. Furthermore, G-268-2 contained the mcr-1 gene, combining phenotypical carbapenem- as well as colistin resistance in one isolate. Carbapenem-resistant Enterobacteriaceae have been found in three out of 45 investigated chicken flocks. This finding is alarming and emphasizes the importance of intervention strategies to contain the environmental spread of resistant bacteria in animals and humans.

  19. Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis

    Directory of Open Access Journals (Sweden)

    Nicole Roschanski

    2018-03-01

    Full Text Available Carbapenems are last-resort antibiotics used in human medicine. The increased detection of carbapenem-resistant Enterobacteriaceae (CRE is therefore worrying. In 2011 we reported the first livestock-associated VIM-1-producing Salmonella (S. enterica serovar Infantis (R3 isolate from dust, sampled in a German chicken fattening farm. Due to this observation we retrospectively investigated more than 536 stored bacterial cultures, isolated from 45 chicken fattening farms during the years 2011 and 2012. After a non-selective overnight incubation, the bacteria were transferred to selective media. Escherichia (E. coli and Salmonella growing on these media were further investigated, including antibiotic susceptibility testing, carbapenemase gene screening and whole genome sequencing (WGS. In total, four CRE were found in three out of 45 investigated farms: Besides R3, one additional Salmonella (G-336-1a as well as two E. coli isolates (G-336-2, G-268-2. All but G-268-2 harbored the blaVIM-1 gene. Salmonella isolates R3 and G-336-1 were closely related although derived from two different farms. All three blaVIM-1-encoding isolates possessed identical plasmids and the blaVIM-1- containing transposon showed mobility at least in vitro. In isolate G-268-2, the AmpC beta-lactamase gene blaCMY-2 but no known carbapenemase gene was identified. However, a transfer of the phenotypic resistance was possible. Furthermore, G-268-2 contained the mcr-1 gene, combining phenotypical carbapenem- as well as colistin resistance in one isolate. Carbapenem-resistant Enterobacteriaceae have been found in three out of 45 investigated chicken flocks. This finding is alarming and emphasizes the importance of intervention strategies to contain the environmental spread of resistant bacteria in animals and humans.

  20. "Fraqueza de nascença": sentidos e significados culturais de impressões maternas na saúde infantil no Nordeste brasileiro "Birth weakness": cultural meanings of maternal impressions for infant health in Northeast Brazil

    Directory of Open Access Journals (Sweden)

    Paola Gondim Calvasina

    2007-02-01

    Full Text Available A gestação abrange dimensões sócio-culturais, históricas e afetivas, que processam no corpo das mulheres diversos sentidos e significados. Cada sociedade constrói concepções, práticas ou modelos explicativos populares, que se diferenciam do modelo biomédico e visam a proteger a mãe-feto, promovendo uma gravidez saudável. Esta pesquisa qualitativa, baseada na antropologia interpretativa, desvela as experiências vivenciadas por 27 mulheres pobres e suas repercussões na desnutrição dos filhos atendidos num Centro de Desnutrição Infantil em Fortaleza, Nordeste brasileiro. De janeiro a junho de 2004, foram realizadas entrevistas etnográficas e narrativas sobre fraqueza de nascença e observação-participante de cuidados no ambulatório, enfermaria e domicílios. Conforme se argumenta, as mães acreditam que seus sofrimentos físicos, emocionais e a precária condição nutricional é "impressa" no feto, resultando na desnutrição do filho. Enquanto a etnoetiologia da "fraqueza" aponta fatores externos do corpo que involuntariamente atingem a gestante, a visão médica relatada tende a culpabilizar a mãe. É preciso compreender a narrativa das mães, sensibilizar-se com o seu sofrimento e aproximar as concepções populares e biomédicas.Pregnancy encompasses socio-cultural, historical, and affective dimensions that process various meanings in women's bodies. Each society constructs popular concepts, practices, and explanatory models that differ from the biomedical model and aim to protect the mother and fetus and foster a healthy pregnancy. This qualitative study, based on interpretative anthropology, unveils the experiences of 27 poor women and their repercussions on the malnutrition of their infants, treated at a Childhood Malnutrition Treatment Center in Fortaleza, Northeast Brazil. From January to June 2004, ethnographic and narrative interviews were conducted on so-called "birth weakness", in addition to participant

  1. MEAN OF MEDIAN ABSOLUTE DERIVATION TECHNIQUE MEAN ...

    African Journals Online (AJOL)

    eobe

    development of mean of median absolute derivation technique based on the based on the based on .... of noise mean to estimate the speckle noise variance. Noise mean property ..... Foraging Optimization,” International Journal of. Advanced ...

  2. Culture and social class.

    Science.gov (United States)

    Miyamoto, Yuri

    2017-12-01

    A large body of research in Western cultures has demonstrated the psychological and health effects of social class. This review outlines a cultural psychological approach to social stratification by comparing psychological and health manifestations of social class across Western and East Asian cultures. These comparisons suggest that cultural meaning systems shape how people make meaning and respond to material/structural conditions associated with social class, thereby leading to culturally divergent manifestations of social class. Specifically, unlike their counterparts in Western cultures, individuals of high social class in East Asian cultures tend to show high conformity and other-orientated psychological attributes. In addition, cultures differ in how social class impacts health (i.e. on which bases, through which pathways, and to what extent). Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Wordplay in Advertising: Form, Meaning and Function

    Directory of Open Access Journals (Sweden)

    Sara Laviosa

    2015-10-01

    Full Text Available Generally speaking, wordplay (or pun is a witticism that relies for its effect on playing with different levels of language, i.e. phonological, graphological, morphological, lexical, syntactic, and textual. Puns are frequently used in commercial advertising as a rhetorical device to promote a given product or service by creating humour, attracting the reader’s attention and adding persuasive force to the message. They also reflect the cultural preferences and traditions of a country, therefore they can be fruitfully used for pedagogic purposes to raise awareness of the specific linguistic and cultural features of the foreign language. In this paper  I examine the form, meaning and function of puns that rely on the different meanings of polysemic words, the literal and non-literal senses of idioms or on bringing two homonyms together in the same utterance to produce witty remarks. After introducing the notions of homonymy, polysemy and idiom I analyse the play on words contained in a sample of advertisements selected at random from two broadsheet newspapers, The Guardian Weekend (1997 and The Independent (1997, two quality weekly magazines, Cosmopolitan (1997 and The Telegraph Magazine (1997, as well as a promotional brochure by Alliance & Leicester plc (2003 and promotional leaflets published in 2000 respectively by Royal Mail, British Telecommunications plc and Johnson & Johnson. Finally, I propose some activities that can be carried out in the LSP classroom either individually or in groups to raise awareness of some of the linguistic and cultural features characterizing the rhetoric of marketing and promotion in business language.

  4. USO DE CULTURAS INICIADORAS PARA A ELABORAÇÃO DE UM EMBUTIDO À BASE DE CARNE DE PATO (Cairina moschata USE OF STARTER CULTURES TO PRODUCE FERMENTED SAUSAGE FROM DUCK MEAT

    Directory of Open Access Journals (Sweden)

    Felipe Oliveira CARIONI

    2001-12-01

    industry, being directly related to the final product flavor, texture, colour and shelf life. The use of starter cultures to produce fermented sausages with a base of duck meat from the boning of thighs and real thighs was evaluated in this research. A mixture of Lactobacillus plantarum BN and Kokuria varians CCT 4492 starter cultures was used to inoculate the meat mixture. The sausages were smoked in a smoking chamber at 23±1°C for approximately 19 hours and were cured for 25 days. The initial counting of viable cells in the sausage mixture was 6.08Log10 CFU/g and 6.04Log10 CFU/g for lactic acid bacteria and Micrococcacea, respectively. Acid lactic bacteria displayed a growth of 0.79 logarithm cycles after the second day of processing, and an increase of 2.58 logarithm cycles at the 11th day. The final product acidity average value in lactic acid was 0,39%, and the pH value, 5.11. The physico-chemical analyses rested within Brazilian Legal Standards. The final product presented a sensorial profile within acceptable quality standards.

  5. Estudo da cultura canavieira na região de Piracicaba por fotointerpretação: Parte II A study on sugarcane cultures in the Piracicaba region by means of photointerpretation: Part II

    Directory of Open Access Journals (Sweden)

    Raul Audi

    1968-01-01

    Full Text Available Dando prosseguimento ao Estudo da Cultura Canavieira na região de Piracicaba, são apresentadas, com base no estudo de fotografias aéreas, as principais características dessa cultura no ano de 1962. Considerando-se os municípios de Rio das Pedras, Mombuca, Capivari e Rafard, foram determinados os dados de áreas cultivadas, localização das culturas e usinas, aspectos das culturas e dos terrenos empregados.Pursuing research work about sugarcane cultures in the region of Piracicaba, their main characteristics were studied in this paper, for the year 1962, based on aerial photographs. Taking into consideration such districts as Rio das Pedras, Mombuca, Capivari and Rafard, a large number of data was determined, of areas under cultivation, localization of cultures and factories, aspects of cultures and of lands used for this crop.

  6. Uranium leaching using mixed organic acids produced by Aspergillus niger

    International Nuclear Information System (INIS)

    Yong-dong Wang; Guang-yue Li; De-xin Ding; Zhi-xiang Zhou; Qin-wen Deng; Nan Hu; Yan Tan

    2013-01-01

    Both of culture temperature and pH value had impacts on the degree of uranium extraction through changing types and concentrations of mixed organic acids produced by Aspergillus niger, and significant interactions existed between them though pH value played a leading role. And with the change of pH value of mixed organic acids, the types and contents of mixed organic acids changed and impacted on the degree of uranium extraction, especially oxalic acid, citric acid and malic acid. The mean degree of uranium extraction rose to peak when the culture temperature was 25 deg C (76.14 %) and pH value of mixed organic acids was 2.3 (82.40 %) respectively. And the highest one was 83.09 %. The optimal culture temperature (25 deg C) of A. niger for uranium leaching was different from the most appropriate growing temperature (37 deg C). (author)

  7. Method of producing molybdenum-99

    Science.gov (United States)

    Pitcher, Eric John

    2013-05-28

    Method of producing molybdenum-99, comprising accelerating ions by means of an accelerator; directing the ions onto a metal target so as to generate neutrons having an energy of greater than 10 MeV; directing the neutrons through a converter material comprising techentium-99 to produce a mixture comprising molybdenum-99; and, chemically extracting the molybdenum-99 from the mixture.

  8. O significado cultural atribuído ao centro de terapia intensiva por clientes e seus familiares: um elo entre a beira do abismo e a liberdade El significado cultural atribuido al proceso de hospitalización en centro de terapia intensiva por clientes y sus familiares: un eslabón entre el borde del abismo y la libertad The cultural meaning attributed to the intensive care unit by clients and their parents: a link between abysm border and freedom

    Directory of Open Access Journals (Sweden)

    Rejane Cussi Assunção Lemos

    2002-06-01

    Full Text Available Este estudo etnográfico teve como objetivo identificar os significados culturais que os clientes admitidos em Centro de Terapia Intensiva e seus familiares atribuem ao processo de hospitalização. Os dados foram coletados mediante a realização de observação participante e entrevistas com 17 sujeitos (clientes e seus familiares, durante a hospitalização. A partir da análise dos dados, surgiram três categorias: 1 CTI, a beira do abismo, um lugar desconhecido e assustador; 2 O elo entre a beira do abismo e a liberdade; 3 A alta do CTI: a liberdade para a vida. O tema central foi: "CTI, um elo entre a beira do abismo e a liberdade". Considerou-se relevante desvelar os significados culturais envolvidos nessa situação, o que poderá subsidiar uma assistência personalizada ao cliente e sua família.Este estudio tiene como objetivo identificar los significados culturales que los clientes admitidos en Centro de Terapia Intensiva (CTI y sus familiares atribuyen al proceso de hospitalización. Los datos fueron recogidos mediante la realización de observación participante y entrevistas con 17 participantes (clientes y sus familiares, durante la hospitalización. Del análisis de los datos emergieron tres categorías principales: 1 CTI, al borde del abismo: un sitio desconocido y asustador; 2 CTI: el eslabón entre el borde del abismo y la libertad; 3 El alta del CTI: la libertad para la vida. El tema central fue: "CTI: un eslabón entre el borde del abismo y la libertad". Se consideró relevante revelar los significados culturales envueltos en esta situación concediendo una asistencia personalizada al cliente y su familia.The purpose of this ethnographic study was to identify the cultural meaning that patients in an intensive care unit and their relatives attribute to the hospitalization process. Data were collected by means of participant observation and interviews with 17 participants (clients and their family, during hospitalization. Data

  9. DIAGNOSIS OF CULTURAL ORGANIZATIONS

    Directory of Open Access Journals (Sweden)

    ALBU MĂDĂLINA

    2015-04-01

    Full Text Available Cultural institution management is to direct the organization to a specific cultural profile purpose, namely production values esthetic sense, artistic, moral, spiritual, distribution, promotion of these values, protection and circulation of cultural heritage. In this regard, an analysis in the diagnosis cultural organizations aims to determine the main strengths and weaknesses, assess the potential and making recommendations focused on the root causes of failures and positive aspects. This paper presents considerations diligence activity Culture House "IL Caragiale "in Ploiesti. The mission of this organization is to contribute to the cultural development of the community by initiating projects and cultural programs, offer development programs and services to meet cultural needs, increase public access to diverse cultural life, providing a constant presence institution circuit local, national, European and international level. Conclusions drawn from the analysis shows that in a world of economic globalization, information and culture in a company in constant change, in a competitive market where there is information readily available means and leisure, but not cultural consistency in a social environment where interest in culture of people is declining, the situation of the population is impaired, the remuneration of staff working in the field of cultural education is demotivating, the funds allocated to culture have grown lately effectively lead a cultural institution is a challenge.

  10. Culture-sensitive psychotraumatology

    Directory of Open Access Journals (Sweden)

    Ulrich Schnyder

    2016-07-01

    Full Text Available Background: Although there is some evidence of the posttraumatic stress disorder (PTSD construct's cross cultural validity, trauma-related disorders may vary across cultures, and the same may be true for treatments that address such conditions. Experienced therapists tailor psychotherapy to each patient's particular situation, to the nature of the patient's psychopathology, to the stage of therapy, and so on. In addition, culture-sensitive psychotherapists try to understand how culture enhances the meaning of their patient's life history, the cultural components of their illness and help-seeking behaviors, as well as their expectations with regard to treatment. We cannot take for granted that all treatment-seeking trauma survivors speak our language or share our cultural values. Therefore, we need to increase our cultural competencies. Methods: The authors of this article are clinicians and/or researchers from across the globe, working with trauma survivors in various settings. Each author focused on one or more specific cultural aspects of working with trauma survivors and highlighted the following aspects. Results: As a result of culture-specific individual and collective meanings linked to trauma and trauma-related disorders survivors may be exposed to (self-stigma in the aftermath of trauma. Patients who are reluctant to talk about their traumatic experiences may instead be willing to write or use other ways of accessing the painful memories such as drawing. In other cultures, community and family cohesion are crucial elements of recovery. While awareness of culture-specific aspects is important, we also need to beware of premature cultural stereotyping. When disseminating empirically supported psychotherapies for PTSD across cultures, a number of additional challenges need to be taken into account: many low and middle income countries have very limited resources available and suffer from a poor health infrastructure. Conclusions: In summary

  11. Culture-specific delusions. Sense and nonsense in cultural context.

    Science.gov (United States)

    Gaines, A D

    1995-06-01

    It can be said that a definition of delusions requires the invocation of cultural understandings, standards of acceptability, as well as conceptions of reality and the forces that animate it. For these reasons, the determination of delusional or normative ideation can only be effected properly within particular cultural contexts. The cross-cultural record suggests that it is difficult to separate the delusional from the cultural; a belief that is patterened and culturally specific is, by definition a cultural, not a delusional belief. One must rely upon particular, relevant local cultural understandings to ascertain when the bounds of culture have been transgressed and meaning has given way to unshareable nonsense.

  12. Tomboys: meanings, marginalization, and misunderstandings.

    Science.gov (United States)

    Hall, Joanne M

    2008-06-01

    Girls who act like boys are called "tomboys." This is an exploration using feminist guiding principles, about what "tomboyism" means to children and how adults around them construct it. The purpose is to examine tomboyism from literary, linguistic, historical and cultural perspectives. The exploration includes a review of selected scientific sources, children's and some adult literature, and conversations with adult women who recall their tomboyism from youth and how it now affects them as adults.

  13. The dance of meaning

    DEFF Research Database (Denmark)

    Rasmussen, Ole Elstrup

    2005-01-01

    competence, qualifications, sense making, reasoning, meaning, intentionality, interpersonal relationship......competence, qualifications, sense making, reasoning, meaning, intentionality, interpersonal relationship...

  14. CHALLENGES IN CROSS CULTURAL ADVERTISING

    OpenAIRE

    Yuni Retnowati

    2016-01-01

    At first, marketing practitioners and academics consider standardized approaches to marketing and advertising strategies in globalization, and then some studies proved that the standardization of advertising across culture is not valid. Therefore, cross cultural advertising takes local culture into account when conveying messages in advertisements. Cross cultural understanding is very important in order to produce successful localized advertising that would reflect the cultural values and nor...

  15. Experiencing Global Culture in Vatel

    DEFF Research Database (Denmark)

    Kampf, Constance Elizabeth; Broillet, Alexandra; John, Claudette

    2015-01-01

    To explore the meaning of “global culture” in a professional communication context, this paper explores the “unsettled” global culture of Vatel, a private business school educating students from nearly 50 different countries for the hospitality industry. This paper explores the role of knowledge...... management in understanding global culture, arguing that the notion of “unsettled” cultures demonstrates how ideologies function in global settings and draw on national “settled” cultural resources. In unpacking different aspects of Vatel's culture this paper questions assumptions built into cultural frames...... of reference by offering a global culture frame, drawing on cultural resources from country based “settled” cultural contexts, and reflects on how global cultural contexts can benefit from a knowledge management approach to this dynamic between “settled” cultural contexts and “unsettled” cultural ideologies...

  16. Spiritual meaning culturocentric education

    Directory of Open Access Journals (Sweden)

    O. H. Rohova

    2014-06-01

    The spiritually­values educational sense of culture consists in changes that take place in personality during the process of culture­centering education that offers «modernisation» of civilizations component of its maintenance due to the opening of its spiritually­values essence where the symphony of secular and religious cultures acquires a value that assists providing of firmness of personality in conditions of Postmodern.

  17. 7 CFR 1206.16 - Producer.

    Science.gov (United States)

    2010-01-01

    ... producing, or causing to be produced, mangos beyond the person's own family use and having value at first... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE MANGO PROMOTION, RESEARCH, AND INFORMATION Mango Promotion, Research, and Information Order Definitions § 1206.16 Producer. Producer means...

  18. CULTURAL ISSUES IN ECONOMICS

    OpenAIRE

    Maciej Meyer

    2012-01-01

    This article has been written with the purpose of attracting attention to the cultural issues, or rather lack of them, in economics. This topic has not been taken frequently into theoretical considerations due to some difficulties, although its practical implications are of great importance. The meaning of institutions which are a part of cultures has been given more coverage in the literature. The following hypothesis is proposed: culture is an important but underestimated component of the e...

  19. [Darwinism and the meaning of "meaning"].

    Science.gov (United States)

    Castrodeza, Carlos

    2009-01-01

    The problem of the meaning of life is herewith contemplated from a Darwinian perspective. It is argued how factors such as existential depression, the concern about the meaning of "meaning," the problem of evil, death as the end of our personal identity, happiness as an unachievable goal, etc. may well have an adaptive dimension "controlled" neither by ourselves nor obscure third parties (conspiracy theories) but "simply" by our genes (replicators in general) so that little if anything is to be done to find a radical remedy for the human condition.

  20. Conceptualizing leadership across cultures

    NARCIS (Netherlands)

    Dickson, M.W.; Castaño, N.; Magomaeva, A.; den Hartog, D.N.

    2012-01-01

    In this article, we summarize research on how the meaning of leadership varies systematically across cultures, and describe the conflict in the literature between the quest for universals and the identification of cultural contingencies in leadership theory. We review the literature on the

  1. Cultural Diplomacy in Europe.

    Science.gov (United States)

    Haigh, Anthony

    The evolution of European government activities in the sphere of international cultural relations is examined. Section 1 describes the period between World War I and World War II when European governments tried to enhance their prestige and policies by means of cultural propaganda. Section 2 analyzes the period during World War II when the…

  2. Myth, Metaphors, and Meaning.

    Science.gov (United States)

    Simpson, Judith W.

    In post-medieval times, art became sacrosanct rather than special as it had been regarded in previous cultures. This notion still drives art education and prevents the inclusion of entire cultures in contemporary curricula in art education. The myths that any society lives with, whether religious, political, or economic, are embedded in that…

  3. Oscillating Cell Culture Bioreactor

    Science.gov (United States)

    Freed, Lisa E.; Cheng, Mingyu; Moretti, Matteo G.

    2010-01-01

    To better exploit the principles of gas transport and mass transport during the processes of cell seeding of 3D scaffolds and in vitro culture of 3D tissue engineered constructs, the oscillatory cell culture bioreactor provides a flow of cell suspensions and culture media directly through a porous 3D scaffold (during cell seeding) and a 3D construct (during subsequent cultivation) within a highly gas-permeable closed-loop tube. This design is simple, modular, and flexible, and its component parts are easy to assemble and operate, and are inexpensive. Chamber volume can be very low, but can be easily scaled up. This innovation is well suited to work with different biological specimens, particularly with cells having high oxygen requirements and/or shear sensitivity, and different scaffold structures and dimensions. The closed-loop changer is highly gas permeable to allow efficient gas exchange during the cell seeding/culturing process. A porous scaffold, which may be seeded with cells, is fixed by means of a scaffold holder to the chamber wall with scaffold/construct orientation with respect to the chamber determined by the geometry of the scaffold holder. A fluid, with/without biological specimens, is added to the chamber such that all, or most, of the air is displaced (i.e., with or without an enclosed air bubble). Motion is applied to the chamber within a controlled environment (e.g., oscillatory motion within a humidified 37 C incubator). Movement of the chamber induces relative motion of the scaffold/construct with respect to the fluid. In case the fluid is a cell suspension, cells will come into contact with the scaffold and eventually adhere to it. Alternatively, cells can be seeded on scaffolds by gel entrapment prior to bioreactor cultivation. Subsequently, the oscillatory cell culture bioreactor will provide efficient gas exchange (i.e., of oxygen and carbon dioxide, as required for viability of metabolically active cells) and controlled levels of fluid

  4. The effect of cultural interaction on cumulative cultural evolution.

    Science.gov (United States)

    Nakahashi, Wataru

    2014-07-07

    Cultural transmission and cultural evolution are important for animals, especially for humans. I developed a new analytical model of cultural evolution, in which each newborn learns cultural traits from multiple individuals (exemplars) in parental generation, individually explores around learned cultural traits, judges the utility of known cultural traits, and adopts a mature cultural trait. Cultural evolutionary speed increases when individuals explore a wider range of cultural traits, accurately judge the skill level of cultural traits (strong direct bias), do not strongly conform to the population mean, increase the exploration range according to the variety of socially learned cultural traits (condition dependent exploration), and make smaller errors in social learning. Number of exemplars, population size, similarity of cultural traits between exemplars, and one-to-many transmission have little effect on cultural evolutionary speed. I also investigated how cultural interaction between two populations with different mean skill levels affects their cultural evolution. A population sometimes increases in skill level more if it encounters a less skilled population than if it does not encounter anyone. A less skilled population sometimes exceeds a more skilled population in skill level by cultural interaction between both populations. The appropriateness of this analytical method is confirmed by individual-based simulations. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Influencing agent group behavior by adjusting cultural trait values.

    Science.gov (United States)

    Tuli, Gaurav; Hexmoor, Henry

    2010-10-01

    Social reasoning and norms among individuals that share cultural traits are largely fashioned by those traits. We have explored predominant sociological and cultural traits. We offer a methodology for parametrically adjusting relevant traits. This exploratory study heralds a capability to deliberately tune cultural group traits in order to produce a desired group behavior. To validate our methodology, we implemented a prototypical-agent-based simulated test bed for demonstrating an exemplar from intelligence, surveillance, and reconnaissance scenario. A group of simulated agents traverses a hostile territory while a user adjusts their cultural group trait settings. Group and individual utilities are dynamically observed against parametric values for the selected traits. Uncertainty avoidance index and individualism are the cultural traits we examined in depth. Upon the user's training of the correspondence between cultural values and system utilities, users deliberately produce the desired system utilities by issuing changes to trait. Specific cultural traits are without meaning outside of their context. Efficacy and timely application of traits in a given context do yield desirable results. This paper heralds a path for the control of large systems via parametric cultural adjustments.

  6. Saying What You Mean without Being Mean

    Science.gov (United States)

    Reilly, Marceta

    2016-01-01

    There's bad news and good news about feedback and teachers collaborating: Getting feedback on our performance is a great way to grow as educators--but feedback often backfires and doesn't produce change in the person getting the feedback. Reilly notes that there are two components to a feedback exchange: the content--the message the person…

  7. The meaning of peer culture for learning at school: the example of a student company / O significado da cultura de pares para a aprendizagem na escola: o exemplo de uma empresa júnior

    Directory of Open Access Journals (Sweden)

    Barbara Asbrand

    2011-06-01

    Full Text Available The empirical research investigates the orientation and learning processes of adolescents concerning global issues in different educational settings. How do adolescents create their knowledge about the world? What worldviews and ideas do adolescents have about global perspectives? How do they deal with the complexity of world society? The qualitative-empirical research focuses on the comparative analysis of learning processes in different educational settings, such as school lessons in different subjects, school-based extra-curricular activities and non-formal youth work outside school. The main topic of the paper is a case study of a group of female students who run World Shop as student company. The objective is to describe a specific learning culture at a gymnasium, a German grammar school, and the learning processes which occur within a certain learning arrangement. In this context, the student company is important both as an extra-curricular project and because issues which occur in its work setting are integrated into different school lessons. The integration of Global Education in school culture results from the presence of the student company in everyday life at school and the combination of informal learning processes within the peer milieu and formal systematic instruction in school lessons. The research reveals the great potential for the desired acquisition of competencies and knowledge. This in turn demonstrates the extent that student learning is encouraged by a particular school and learning culture. A investigação empirica investiga a orientação e o processo de aprendizagem de adolescentes referente a questões globais em diferentes contextos educacionais. Como adolescentes criam seu conhecimento do mundo? Quais as visões de mundo e as ideias que os adolescentes tem a partir da perspectiva global? Como lidam com a complexidade da sociedade mundial? A pesquisa qualitativa-empirica foca na análise comparativa dos processos em

  8. Meaning through Things

    Science.gov (United States)

    Johnson, Marilynn

    2017-01-01

    Interpretation is the process by which we find meaning in the things in the world around us: clouds on the horizon, bones, street signs, hairbrushes, uniforms, paintings, letters, and utterances. But where does that meaning come from and on what basis are we justified in saying a particular meaning is the right meaning? Drawing from debates in the…

  9. Culture-sensitive psychotraumatology

    Science.gov (United States)

    Schnyder, Ulrich; Bryant, Richard A.; Ehlers, Anke; Foa, Edna B.; Hasan, Aram; Mwiti, Gladys; Kristensen, Christian H.; Neuner, Frank; Oe, Misari; Yule, William

    2016-01-01

    Background Although there is some evidence of the posttraumatic stress disorder (PTSD) construct's cross cultural validity, trauma-related disorders may vary across cultures, and the same may be true for treatments that address such conditions. Experienced therapists tailor psychotherapy to each patient's particular situation, to the nature of the patient's psychopathology, to the stage of therapy, and so on. In addition, culture-sensitive psychotherapists try to understand how culture enhances the meaning of their patient's life history, the cultural components of their illness and help-seeking behaviors, as well as their expectations with regard to treatment. We cannot take for granted that all treatment-seeking trauma survivors speak our language or share our cultural values. Therefore, we need to increase our cultural competencies. Methods The authors of this article are clinicians and/or researchers from across the globe, working with trauma survivors in various settings. Each author focused on one or more specific cultural aspects of working with trauma survivors and highlighted the following aspects. Results As a result of culture-specific individual and collective meanings linked to trauma and trauma-related disorders survivors may be exposed to (self-)stigma in the aftermath of trauma. Patients who are reluctant to talk about their traumatic experiences may instead be willing to write or use other ways of accessing the painful memories such as drawing. In other cultures, community and family cohesion are crucial elements of recovery. While awareness of culture-specific aspects is important, we also need to beware of premature cultural stereotyping. When disseminating empirically supported psychotherapies for PTSD across cultures, a number of additional challenges need to be taken into account: many low and middle income countries have very limited resources available and suffer from a poor health infrastructure. Conclusions In summary, culture

  10. Sound as Popular Culture

    DEFF Research Database (Denmark)

    The wide-ranging texts in this book take as their premise the idea that sound is a subject through which popular culture can be analyzed in an innovative way. From an infant’s gurgles over a baby monitor to the roar of the crowd in a stadium to the sub-bass frequencies produced by sound systems...... in the disco era, sound—not necessarily aestheticized as music—is inextricably part of the many domains of popular culture. Expanding the view taken by many scholars of cultural studies, the contributors consider cultural practices concerning sound not merely as semiotic or signifying processes but as material......, physical, perceptual, and sensory processes that integrate a multitude of cultural traditions and forms of knowledge. The chapters discuss conceptual issues as well as terminologies and research methods; analyze historical and contemporary case studies of listening in various sound cultures; and consider...

  11. Politics of Meaning in Mathematics Education

    DEFF Research Database (Denmark)

    Skovsmose, Ole

    2016-01-01

    By a politics of meaning I refer to the social, economic, cultural and religious conditions for experiencing meaning. I refer as well to the layers of visons, assumptions, presumptions and preconceptions that might construct something as being meaningful. By addressing different politics of meani...... of such factors. Politics of meaning can be analysed with reference to sexism, racism, instrumentalism, the school mathematics tradition, critical mathematics education, and the banality of expertise....

  12. Safeguards Culture

    Energy Technology Data Exchange (ETDEWEB)

    Frazar, Sarah L.; Mladineo, Stephen V.

    2012-07-01

    The concepts of nuclear safety and security culture are well established; however, a common understanding of safeguards culture is not internationally recognized. Supported by the National Nuclear Security Administration, the authors prepared this report, an analysis of the concept of safeguards culture, and gauged its value to the safeguards community. The authors explored distinctions between safeguards culture, safeguards compliance, and safeguards performance, and evaluated synergies and differences between safeguards culture and safety/security culture. The report concludes with suggested next steps.

  13. Organizational culture

    OpenAIRE

    Schein, Edgar H.

    1988-01-01

    Cultural orientations of an organization can be its greatest strength, providing the basis for problem solving, cooperation, and communication. Culture, however, can also inhibit needed changes. Cultural changes typically happen slowly – but without cultural change, many other organizational changes are doomed to fail. The dominant culture of an organization is a major contributor to its success. But, of course, no organizational culture is purely one type or another. And the existence of sec...

  14. Significados culturalmente construidos para el consumo de bebidas azucaradas entre escolares de la Ciudad de México Culturally constructed meanings for consumption of sweetened beverages among schoolchildren in Mexico City

    Directory of Open Access Journals (Sweden)

    Florence Théodore

    2011-10-01

    Full Text Available OBJETIVO: Demostrar la importancia de los factores culturales que hoy motivan a los niños mexicanos a consumir bebidas azucaradas y examinar sus implicaciones para el diseño de programas de promoción de estilos de vida saludable. MÉTODOS: Estudio cualitativo fenomenológico. Se llevaron a cabo nueve entrevistas con pares y cuatro grupos de discusión con niños de entre 9 y 10 años de edad en cuatro escuelas públicas del sur de la Ciudad de México. Las entrevistas se realizaron con apoyo de nueve fotografías de bebidas disponibles en las escuelas y en los hogares. Se buscó identificar las reglas culinarias asociadas con el consumo de bebidas azucaradas y las diferentes valoraciones que hacen los niños acerca de las bebidas. Las entrevistas y los grupos de discusión se grabaron y transcribieron en su integridad. Se desarrollaron matrices de análisis en categorías temáticas identificadas durante el estudio. El análisis se basó en la "comparación continua" de los discursos de niños y niñas, y entre los alumnos de las cuatro escuelas. RESULTADOS: Dos principales elementos de índole sociocultural, construidos en un marco cultural determinado, explican en parte el actual patrón de consumo entre los niños. El primero, una casi inexistente conceptualización de los niños con respecto al consumo de agua, confinada a la realización de actividad física, contraria a la amplia gama de circunstancias y ocasiones que encontraron para el consumo de alguna bebida azucarada. Segundo, la identificación de tres principios que parecen estructurar el consumo de las bebidas -combinación de alimentos salados con bebidas dulces, rol protagónico de las bebidas azucaradas en eventos sociales y asociación estrecha del consumo de agua con la sed provocada por el esfuerzo físico. CONCLUSIONES: Los resultados ponen en evidencia la importancia de considerar el papel de los elementos socialmente significativos en las prácticas alimentarias y la

  15. Internalized Meaning Factualism

    DEFF Research Database (Denmark)

    Hohwy, Jakob

    2006-01-01

    The normative character of meaning creates deep problems for the attempt to give a reductive explanation of the constitution of meaning. I identify and critically examine an increasingly popular Carnap-style position, which I call Internalized Meaning Factualism (versions of which I argue...... are defended by, e.g., Robert Brandom, Paul Horwich and Huw Price), that promises to solve the problems. According to this position, the problem of meaning can be solved by prohibiting an external perspective on meaning constituting properties. The idea is that if we stick to a perspective on meaning...

  16. Citizenship and cultural diversity in agenda of cultural policies

    Directory of Open Access Journals (Sweden)

    Patricia Silva Dorneles

    2016-01-01

    Full Text Available This is a discussion paper which aims to contribute to the systematization of studies, concepts and practices on cultural policies which have been developed in previous years in Brazil and are orienting cultural actions and public programs in the country, also influencing the Occupational Therapy. Citizenship and Cultural Diversity are concepts under construction and are part of the of the agenda of cultural policies and as well as the reflections and practices of various occupational therapists who are acting in a constant dialogue with the cultural area by means of the formation in cultural management, cultural mapping, programs and grant projects aimed to promote inventive identities, traditional communities, native populations, urban mobility, and cultural networks and exchange initiatives, among others. The article presents the process of this conceptual construction and the constitution of experiences aiming the democratization of the culture in the history of Brazilian cultural public policies, over which are being discussed approach paths and possibilities for Occupational Therapy.

  17. What Is so "Cultural" About Cultural Entrepreneurship

    Directory of Open Access Journals (Sweden)

    Staffan Albinsson

    2017-04-01

    Full Text Available The term “cultural entrepreneurship” has been increasingly used during the new millennium, mirroring the rapidly growing importance of the “quaternary sector of the economy,” i.e. knowledge-based industries, including culture. Exploration of the literature in which the term “cultural entrepreneurship” is used does not bring a solid, clear-cut, and unambiguous understanding of its definition or meaning. The aim of this paper is to present various uses of the concept and to bring about some clarity in how the concept can be understood. Two overarching uses of the cultural entrepreneurship concept have been found: 1. the anthropologist’s and institutional economist’s use, which indicates the dynamic development of intangible cultural features such as symbols, myths, languages, beliefs, values, norms, rituals, and attitudes in and between societies, and 2. the arts development use, which indicates the dynamic development of cultural services, tangible goods, and individual or collective career promotion. Most authors use a cultural entrepreneurship concept without defining it. Authors could apply more precise definitions by using a quadruple bottom-line framework to position themselves in the nexus of financial, social, artistic, and cultural perspectives.

  18. Marketing Hardwoods to Furniture Producers

    Science.gov (United States)

    Steven A. Sinclair; Robert J. Bush; Philip A. Araman

    1989-01-01

    This paper discusses some of the many problems in developing marketing programs for small wood products manufacturers. It examines the problems of using price as a dominant means for getting and attracting customers. The marketing of hardwood lumber to furniture producers is then used as an example. Data from 36 furniture lumber buyers is presented to illustrate...

  19. Means on scattered compacta

    Directory of Open Access Journals (Sweden)

    Banakh T.

    2014-01-01

    Full Text Available We prove that a separable Hausdor_ topological space X containing a cocountable subset homeomorphic to [0, ω1] admits no separately continuous mean operation and no diagonally continuous n-mean for n ≥ 2.

  20. Cultivos de alta densidad celular por retención interna: aplicación a la fermentación continua de etanol High cell density cultures produced by internal retention: application in continuous ethanol fermentation

    Directory of Open Access Journals (Sweden)

    Godoy Rubén Darío

    2004-12-01

    Full Text Available El etanol ha generado gran interés por su potencial como combustible alternativo. No obstante, para que este producto sea competitivo económicamente, es necesario desarrollar procesos de fermentación que incrementen la baja productividad volumétrica lograda en cultivos convencionales (por lote o continuo, por medio de técnicas que permitan altas concentraciones celulares y reduzcan la inhibición por producto. Uno de los métodos empleados frecuentemente involucra la recirculación celular; por ello, en este trabajo se desarrolló un reactor de membrana incorporando un módulo de filtración, con unidades tubulares de 5 u,m en acero inoxidable, dentro de un fermentador de tanque agitado de 3L, para investigar su aplicación en la producción continua de etanol. Los efectos de la concentración celular y la caída de presión transmembranal sobre el flux de permeado fueron evaluados para probar el desempeño del módulo de filtración. Previa selección de las condiciones de fermentación (30 °C, 1,25 -1,75 vvm, pH 4,5, el sistema con retención celular interna fue operado en el cultivo continuo de Saccharomyces cerevisiae a partir de sacarosa. La permeabilidad de las unidades filtrantes fue mantenida mediante la aplicación de pulsos de aire. Más del 97% de las células cultivadas fueron retenidas en el fermentador, alcanzándose una concentración celular de 51 g/L y una productividad promedio de etanol, en el cultivo con retención celular, de 8,51 g/L.h, la cual fue dos veces mayor a la que se obtiene en un cultivo continuo convencional. Palabras clave: reactor de membrana, Saccharomyces cerevisiae, fermentación alcohólica, recirculación celular.Ethanol has provoked great interest due to its potential as an alternative fuel. Nevertheless, fermentation processes must be developed by increasing the low volumetric productivity achieved in conventional cultures (batch or continuous to make this product become economically competitive

  1. Cultural Diversity and Multicultural Education

    Science.gov (United States)

    Gay, Geneva

    2013-01-01

    This discussion examines some of the major issues and attributes of culturally responsive teaching. It begins with explaining my views of culturally responsive teaching and how I incorporate cultural responsiveness in my writing to teach readers what it means. These general conceptual frameworks are followed by a discussion of some specific…

  2. Keeping patients safe in healthcare organizations: a structuration theory of safety culture.

    Science.gov (United States)

    Groves, Patricia S; Meisenbach, Rebecca J; Scott-Cawiezell, Jill

    2011-08-01

    This paper presents a discussion of the use of structuration theory to facilitate understanding and improvement of safety culture in healthcare organizations. Patient safety in healthcare organizations is an important problem worldwide. Safety culture has been proposed as a means to keep patients safe. However, lack of appropriate theory limits understanding and improvement of safety culture. The proposed structuration theory of safety culture was based on a critique of available English-language literature, resulting in literature published from 1983 to mid-2009. CINAHL, Communication and Mass Media Complete, ABI/Inform and Google Scholar databases were searched using the following terms: nursing, safety, organizational culture and safety culture. When viewed through the lens of structuration theory, safety culture is a system involving both individual actions and organizational structures. Healthcare organization members, particularly nurses, share these values through communication and enact them in practice, (re)producing an organizational safety culture system that reciprocally constrains and enables the actions of the members in terms of patient safety. This structurational viewpoint illuminates multiple opportunities for safety culture improvement. Nurse leaders should be cognizant of competing value-based culture systems in the organization and attend to nursing agency and all forms of communication when attempting to create or strengthen a safety culture. Applying structuration theory to the concept of safety culture reveals a dynamic system of individual action and organizational structure constraining and enabling safety practice. Nurses are central to the (re)production of this safety culture system. © 2011 Blackwell Publishing Ltd.

  3. Interdisciplinary: Cultural competency and culturally congruent education for millennials in health professions.

    Science.gov (United States)

    Hawala-Druy, Souzan; Hill, Mary H

    2012-10-01

    The increasingly diverse multicultural and multigenerational student population in the United States requires that educators at all levels develop cultural knowledge, awareness, and sensitivity to help diverse learners fulfill their potential and to avoid cultural misunderstandings that can become obstacles or barriers to learning. The purpose of this study was to design and implement eclectic, creative, evidence-based interdisciplinary educational activities, along with culturally congruent teaching strategies, within a semester-long university course that promoted positive and culturally competent learning outcomes for culturally diverse, largely millennial students. The interdisciplinary course would prepare health professional students with the requisite knowledge and skills, through transformative learning that produces change agents, to provide culturally congruent and quality team-based care to diverse populations. This was a qualitative and quantitative study, which measured students' level of cultural awareness, competence, and proficiency pre and post the educational intervention. Instruments used for data collection included the Inventory for Assessing The Process of Cultural Competence-Student Version (IAPCC-SV) by Campinha-Bacote, course evaluations, students' feedback, and portfolio reflections. The study was conducted at a private academic institution located in the Mid-Atlantic region and the sample population included inter-professional students (N=106) from various health professions including nursing, pharmacy, and allied health sciences. Results from the pre- and post-test IAPCC-SV survey revealed that mean scores increased significantly from pre-test (60.8) to post-test (70.6). Thus, students' levels of cultural competency (awareness, knowledge, skills, desire, encounter) improved post-educational intervention, indicating that the teaching methods used in the course might be applied on a larger scale across the university system to cater to the

  4. 7 CFR 1131.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... Handling Definitions § 1131.13 Producer milk. Producer milk means the skim milk (or the skim equivalent of components of skim milk) and butterfat in milk of a producer that is: (a) Received by the operator of a pool... percentage in paragraph (d)(2) of this section may be increased or decreased by the market administrator if...

  5. 7 CFR 1033.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... Handling Definitions § 1033.13 Producer milk. Producer milk means the skim milk (or the skim equivalent of components of skim milk), including nonfat components, and butterfat in milk of a producer that is: (a... percentages in paragraph (d)(4) of this section may be increased or decreased by the market administrator if...

  6. 7 CFR 1001.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... Handling Definitions § 1001.13 Producer milk. Producer milk means the skim milk (or the skim equivalent of components of skim milk) and butterfat contained in milk of a producer that is: (a) Received by the operator... be increased or decreased by the Market Administrator if the Market Administrator finds that such...

  7. 7 CFR 1126.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... Handling Definitions § 1126.13 Producer milk. Producer milk means the skim milk (or the skim equivalent of components of skim milk), including nonfat components, and butterfat contained in milk of a producer that is...) of this section may be increased or decreased by the market administrator if there is a finding that...

  8. 7 CFR 1006.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... Handling Definitions § 1006.13 Producer milk. Producer milk means the skim milk (or the skim equivalent of components of skim milk) and butterfat contained in milk of a producer that is: (a) Received by the operator... (d) (1) through (3) of this section may be increased or decreased by the market administrator if the...

  9. Meaning in mathematics education

    CERN Document Server

    Valero, Paola; Hoyles, Celia; Skovsmose, Ole

    2005-01-01

    What does it mean to know mathematics? How does meaning in mathematics education connect to common sense or to the meaning of mathematics itself? How are meanings constructed and communicated and what are the dilemmas related to these processes? There are many answers to these questions, some of which might appear to be contradictory. Thus understanding the complexity of meaning in mathematics education is a matter of huge importance. There are twin directions in which discussions have developed - theoretical and practical - and this book seeks to move the debate forward along both dimensions while seeking to relate them where appropriate. A discussion of meaning can start from a theoretical examination of mathematics and how mathematicians over time have made sense of their work. However, from a more practical perspective, anybody involved in teaching mathematics is faced with the need to orchestrate the myriad of meanings derived from multiple sources that students develop of mathematical knowledge.

  10. Cultural entrepreneurship

    NARCIS (Netherlands)

    A. Klamer (Arjo)

    2011-01-01

    textabstractCultural entrepreneurship is a new character in the cultural sector. This paper characterizes the cultural entrepreneur paying homage to the hermeneutic approach of Don Lavoie and others. The challenge is to render the "cultural" meaningful. An invention is the highlighting of the

  11. Industrial cultures

    DEFF Research Database (Denmark)

    Rasmussen, Lauge Baungaard

    1996-01-01

    The chapter deals with different paradigms andtheories of cultural development. The problem toexplain change and methods to analyse developmentin different cultures are presented and discussed.......The chapter deals with different paradigms andtheories of cultural development. The problem toexplain change and methods to analyse developmentin different cultures are presented and discussed....

  12. [Possibility of the spectral analysis of heterogeneous biological systems. The determination of the mycelium concentration of Actinomyces aureofaciens, a producer of tetracycline, cultured on a medium with corn meal].

    Science.gov (United States)

    Korolev, Iu N; Slugina, M D; Makarevich, V G; Telegin, N L

    1979-03-01

    A possibility of using spectroscopy of attenuated total reflection in the IR region for analysis of the heterogenic system consisting of the microorganisms and plant cells is discussed. The method of spectroscopy is proposed for estimating the mycelium concentration of Act. aureofaciens producing tetracycline in the presence of corn meal in the medium. The experimental data confirming this possibility are presented. The peculiar properties of the spectral analysis under these particular conditions are discussed. It is supposed that the method may be used for analysis of heterogenous systems including other microorganisms.

  13. Graphing Inequalities, Connecting Meaning

    Science.gov (United States)

    Switzer, J. Matt

    2014-01-01

    Students often have difficulty with graphing inequalities (see Filloy, Rojano, and Rubio 2002; Drijvers 2002), and J. Matt Switzer's students were no exception. Although students can produce graphs for simple inequalities, they often struggle when the format of the inequality is unfamiliar. Even when producing a correct graph of an…

  14. [Temporal meaning of suffering].

    Science.gov (United States)

    Porée, J

    2015-09-01

    and psychic also time take root in the temporality of "planning", understood to be a general form of openness to the world. But in our minds, "being" means more than "being in the world"; it also means, and by that very token, "being for death". Anguish is the fundamental experience that brings out this structural identity. As such, it lies at the core of Heidegger's conception of temporality. Nonetheless, is death the ultimate truth of time? This is what Paul Ricoeur denies in establishing an internal relationship between time and narration, for narration not only produces a synthesis of time, it also allows us to assume a future which, granted, presupposes death but does not remain its captive. This supports a fourth conception of time, that summarized by the notion of "narrated time". Narrative resources do not; however, cancel the multiple alterations that suffering introduces into temporality, which, by contrast, can be reduced - as we show in the second part of the article in reference to the preceding conceptions - to four descriptive features: dechronologization, reduction of the present to the instant, breakdown of the plan, and disintegration of the narrative. They explain why, in the presence of suffering, life seems more impossible than death. But at the same time, it leaves us with an enigma: Why doesn't the suffering human being always choose death? This enigma, above all, is the enigma of melancholic suffering. Hence, the privileged place given to melancholy in the last part of the article, where psychiatry encounters philosophy and justifies some distinctions that philosophy alone would not have dared make. A case in point is Minkowski's distinction, in Lived Time, between "activity" and "waiting", and also holds for our own distinction, following Minkowski, between planning time and hoping time, where hoping is to be taken in the deeper sense than implied by the common opposition between "hope" and "despair". Copyright © 2015 L

  15. Meanings of thinness and dysfunctional eating in black South ...

    African Journals Online (AJOL)

    meanings of thinness and food refusal may not only vary from culture to culture, but ... the rapidly westernizing socio-cultural context of post-apartheid South Africa. Method: Four .... she first arrived in South Africa as an immigrant from a French-.

  16. Brand Meaning Cocreation

    DEFF Research Database (Denmark)

    Tierney, Kieran D.; Karpen, Ingo; Westberg, Kate

    2016-01-01

    Purpose: The purpose of this paper is to consolidate and advance the understanding of brand meaning and the evolving process by which it is determined by introducing and explicating the concept of brand meaning cocreation (BMCC). Design/methodology/approach: In-depth review and integration...... of literature from branding, cocreation, service systems, and practice theory. To support deep theorizing, the authors also examine the role of institutional logics in the BMCC process in framing interactions and brand meaning outcomes. Findings: Prior research is limited in that it neither maps the process...... of cocreation within which meanings emerge nor provides theoretical conceptualizations of brand meaning or the process of BMCC. While the literature acknowledges that brand meaning is influenced by multiple interactions, their nature and how they contribute to BMCC have been overlooked. Research limitations...

  17. ORGANIZATIONAL CULTURE AND MANAGEMENT CULTURE

    OpenAIRE

    Tudor Hobeanu; Loredana Vacarescu Hobeanu

    2010-01-01

    Communication reveals the importance of organizational culture and management culture supported by the remarkable results in economic and social level of organization. Their functions are presented and specific ways of expression levels of organizational culture and ways of adapting to the requirements of the organization's management culture.

  18. Fatty acid-producing hosts

    Science.gov (United States)

    Pfleger, Brian F; Lennen, Rebecca M

    2013-12-31

    Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally delected. The hosts prefereably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recominantly stable and growth-competent at 37.degree. C. Methods of producing a fatty acid product comprising culturing such hosts at 37.degree. C. are also described.

  19. Parallel k-means++

    Energy Technology Data Exchange (ETDEWEB)

    2017-04-04

    A parallelization of the k-means++ seed selection algorithm on three distinct hardware platforms: GPU, multicore CPU, and multithreaded architecture. K-means++ was developed by David Arthur and Sergei Vassilvitskii in 2007 as an extension of the k-means data clustering technique. These algorithms allow people to cluster multidimensional data, by attempting to minimize the mean distance of data points within a cluster. K-means++ improved upon traditional k-means by using a more intelligent approach to selecting the initial seeds for the clustering process. While k-means++ has become a popular alternative to traditional k-means clustering, little work has been done to parallelize this technique. We have developed original C++ code for parallelizing the algorithm on three unique hardware architectures: GPU using NVidia's CUDA/Thrust framework, multicore CPU using OpenMP, and the Cray XMT multithreaded architecture. By parallelizing the process for these platforms, we are able to perform k-means++ clustering much more quickly than it could be done before.

  20. Cultural neuroscience and psychopathology: prospects for cultural psychiatry.

    Science.gov (United States)

    Choudhury, Suparna; Kirmayer, Laurence J

    2009-01-01

    There is a long tradition that seeks to understand the impact of culture on the causes, form, treatment, and outcome of psychiatric disorders. An early, colonialist literature attributed cultural characteristics and variations in psychopathology and behavior to deficiencies in the brains of colonized peoples. Contemporary research in social and cultural neuroscience holds the promise of moving beyond these invidious comparisons to a more sophisticated understanding of cultural variations in brain function relevant to psychiatry. To achieve this, however, we need better models of the nature of psychopathology and of culture itself. Culture is not simply a set of traits or characteristics shared by people with a common geographic, historical, or ethnic background. Current anthropology understands culture as fluid, flexible systems of discourse, institutions, and practices, which individuals actively use for self-fashioning and social positioning. Globalization introduces new cultural dynamics and demands that we rethink culture in relation to a wider domain of evolving identities, knowledge, and practice. Psychopathology is not reducible to brain dysfunction in either its causes, mechanisms, or expression. In addition to neuropsychiatric disorders, the problems that people bring to psychiatrists may result from disorders in cognition, the personal and social meanings of experience, and the dynamics of interpersonal interactions or social systems and institutions. The shifting meanings of culture and psychopathology have implications for efforts to apply cultural neuroscience to psychiatry. We consider how cultural neuroscience can refine use of culture and its role in psychopathology using the example of adolescent aggression as a symptom of conduct disorder.

  1. Culture-specific familiarity equally mediates action representations across cultures.

    Science.gov (United States)

    Umla-Runge, Katja; Fu, Xiaolan; Wang, Lamei; Zimmer, Hubert D

    2014-01-01

    Previous studies have shown that we need to distinguish between means and end information about actions. It is unclear how these two subtypes of action information relate to each other with theoretical accounts postulating the superiority of end over means information and others linking separate means and end routes of processing to actions of differential meaningfulness. Action meaningfulness or familiarity differs between cultures. In a cross-cultural setting, we investigated how action familiarity influences recognition memory for means and end information. Object directed actions of differential familiarity were presented to Chinese and German participants. Action familiarity modulated the representation of means and end information in both cultures in the same way, although the effects were based on different stimulus sets. Our results suggest that, in the representation of actions in memory, end information is superordinate to means information. This effect is independent of culture whereas action familiarity is not.

  2. Cultural commons and cultural evolution

    OpenAIRE

    Bravo, Giangiacomo

    2010-01-01

    Culture evolves following a process that is akin to biological evolution, although with some significant differences. At the same time culture has often a collective good value for human groups. This paper studies culture in an evolutionary perspective, with a focus on the implications of group definition for the coexistence of different cultures. A model of cultural evolution is presented where agents interacts in an artificial environment. The belonging to a specific memetic group is a majo...

  3. The consequences of "Culture's consequences"

    DEFF Research Database (Denmark)

    Knudsen, Fabienne; Froholdt, Lisa Loloma

    2009-01-01

      In this article, it is claimed that research on cross-cultural crews is dominated by one specific understanding of the concept of culture, which is static, evenly distributed and context-independent. Such a conception of culture may bring some basic order while facing an unknown culture...... review of the theory of Geert Hofstede, the most renowned representative of this theoretical approach. The practical consequences of using such a concept of culture is then analysed by means of a critical review of an article applying Hofstede to cross-cultural crews in seafaring. Finally, alternative...... views on culture are presented. The aim of the article is, rather than to promote any specific theory, to reflect about diverse perspectives of cultural sense-making in cross-cultural encounters. Udgivelsesdato: Oktober...

  4. Mean field games

    KAUST Repository

    Gomes, Diogo A.

    2014-01-06

    In this talk we will report on new results concerning the existence of smooth solutions for time dependent mean-field games. This new result is established through a combination of various tools including several a-priori estimates for time-dependent mean-field games combined with new techniques for the regularity of Hamilton-Jacobi equations.

  5. Phenomenology and Meaning Attribution

    African Journals Online (AJOL)

    John Paley. (2017). Phenomenology as Qualitative Research: A Critical Analysis of Meaning Attribution. ... basic philosophical nature of phenomenological meaning and inquiry, and that he not ... In keeping with the title of my book, Researching. Lived Experience ...... a quantitative social science that can make generalizing.

  6. Mean field games

    KAUST Repository

    Gomes, Diogo A.

    2014-01-01

    In this talk we will report on new results concerning the existence of smooth solutions for time dependent mean-field games. This new result is established through a combination of various tools including several a-priori estimates for time-dependent mean-field games combined with new techniques for the regularity of Hamilton-Jacobi equations.

  7. Mesenchymal stem cells are efficiently transduced with adenoviruses bearing type 35-derived fibers and the transduced cells with the IL-28A gene produces cytotoxicity to lung carcinoma cells co-cultured.

    Science.gov (United States)

    Suzuki, Takeo; Kawamura, Kiyoko; Li, Quanhai; Okamoto, Shinya; Tada, Yuji; Tatsumi, Koichiro; Shimada, Hideaki; Hiroshima, Kenzo; Yamaguchi, Naoto; Tagawa, Masatoshi

    2014-09-25

    Transduction of human mesenchymal stem cells (MSCs) with type 5 adenoviruses (Ad5) is limited in the efficacy because of the poor expression level of the coxsackie adenovirus receptor (CAR) molecules. We examined a possible improvement of Ad-mediated gene transfer in MSCs by substituting the fiber region of type 5 Ad with that of type 35 Ad. Expression levels of CAR and CD46 molecules, which are the major receptors for type 5 and type 35 Ad, respectively, were assayed with flow cytometry. We constructed vectors expressing the green fluorescent protein gene with Ad5 or modified Ad5 bearing the type 35 fiber region (AdF35), and examined the infectivity to MSCs with flow cytometry. We investigated anti-tumor effects of MSCs transduced with interleukin (IL)-28A gene on human lung carcinoma cells with a colorimetric assay. Expression of IL-28A receptors was tested with the polymerase chain reaction. A promoter activity of transcriptional regulatory regions in MSCs was determined with a luciferase assay and a tumor growth-promoting ability of MSCs was tested with co-injection of human tumor cells in nude mice. MSCs expressed CD46 but scarcely CAR molecules, and subsequently were transduced with AdF35 but not with Ad5. Growth of MSCs transduced with the IL-28A gene remained the same as that of untransduced cells since MSCs were negative for the IL-28A receptors. The IL-28A-transduced MSCs however suppressed growth of lung carcinoma cells co-cultured, whereas MSCs transduced with AdF35 expressing the β-galactosidase gene did not. A regulatory region of the cyclooygenase-2 gene possessed transcriptional activities greater than other tumor promoters but less than the cytomegalovirus promoter, and MSCs themselves did not support tumor growth in vivo. AdF35 is a suitable vector to transduce MSCs that are resistant to Ad5-mediated gene transfer. MSCs infected with AdF35 that activate an exogenous gene by the cytomegalovirus promoter can be a vehicle to deliver the gene product

  8. Culture and teams.

    Science.gov (United States)

    Kirkman, Bradley L; Shapiro, Debra L; Lu, Shuye; McGurrin, Daniel P

    2016-04-01

    We first review research on culture effects in teams, illustrating that mean levels of team cultural values have main (i.e. direct) effects, indirect effects (i.e. mediated by intervening variables), and moderating influences on team processes and outcomes. Variance in team cultural values or on country of origin (i.e. nationality diversity) also has main effects on team functioning, and we highlight contextual variables that strengthen or weaken these main effects. We next review research examining the effect of variance in team cultural values on global virtual teams, specifically. Finally, we review research on how cultural values shape employees' receptivity to empowering leadership behavior in teams. We conclude by discussing critical areas for future research. Published by Elsevier Ltd.

  9. Brookhaven Linac Isotope Producer

    Data.gov (United States)

    Federal Laboratory Consortium — The Brookhaven Linac Isoptope Producer (BLIP)—positioned at the forefront of research into radioisotopes used in cancer treatment and diagnosis—produces commercially...

  10. Hemoglobin promotes somatic embryogenesis in peanut cultures.

    Science.gov (United States)

    Jayabalan, N; Anthony, P; Davey, M R; Power, J B; Lowe, K C

    2004-02-01

    Critical parameters influencing somatic embryogenesis include growth regulators and oxygen supply. Consequently, the present investigation has focused on optimization of a somatic embryogenic system for peanut (Arachis hypogaea L.) through media supplementation with the auxin, picloram. The latter at 30 mg L(-1) was optimal for inducing regeneration of somatic embryos from cultured explants of zygotic embryos. In contrast, somatic embryogenesis did not occur in the absence of this growth regulator. An assessment has also been made of the beneficial effect on somatic embryogenesis and plant regeneration of the commercial hemoglobin (Hb) solution, Erythrogen. Hemoglobin at 1:50 and 1:100 (v:v) stimulated increases in mean fresh weight (up to a maximum of 57% over control), mean number of explants producing somatic embryos (15%) and mean number of somatic embryos per explant (29%).

  11. Narrating horror: the horror film as cultural construct

    OpenAIRE

    Haynes, Simon

    1997-01-01

    This thesis examines horror films through an application of cultural analysis (primarily the work of Pierre Bourdieu) to selected texts in order to answer critics employing psychoanalytic perspectives to horror. It argues that psychoanalysis misses much of the heart of horror texts through its claims that textual 'meaning' lies within individuals rather than in the society in which horror texts were produced.\\ud Bourdieu's hypotheses are applied to films, along with the work of more specific ...

  12. Insect cell transformation vectors that support high level expression and promoter assessment in insect cell culture

    Science.gov (United States)

    A somatic transformation vector, pDP9, was constructed that provides a simplified means of producing permanently transformed cultured insect cells that support high levels of protein expression of foreign genes. The pDP9 plasmid vector incorporates DNA sequences from the Junonia coenia densovirus th...

  13. Mean-periodic functions

    Directory of Open Access Journals (Sweden)

    Carlos A. Berenstein

    1980-01-01

    Full Text Available We show that any mean-periodic function f can be represented in terms of exponential-polynomial solutions of the same convolution equation f satisfies, i.e., u∗f=0(μ∈E′(ℝn. This extends to n-variables the work of L. Schwartz on mean-periodicity and also extends L. Ehrenpreis' work on partial differential equations with constant coefficients to arbitrary convolutors. We also answer a number of open questions about mean-periodic functions of one variable. The basic ingredient is our work on interpolation by entire functions in one and several complex variables.

  14. Meaning in Work Life

    DEFF Research Database (Denmark)

    Ravn, Ib

    meaningful is still lacking (Harpaz & Fu, 2002), as are definitions of key terms. Here, I define meaning as the experience that something fits into a wider context or coheres within a larger whole.     I conceptualize meaning in work life as deriving from four factors, each of which helps us contribute...... to something larger than ourselves (Seligman, 2002) through our work. Thus, a person experiences meaning in work life when she... A. is able to use her signature strengths at work (Peterson and Seligman, 2004), B. makes an important contribution to the workplace (Drucker, 1999), C. participates in a productive...

  15. CHALLENGES IN CROSS CULTURAL ADVERTISING

    Directory of Open Access Journals (Sweden)

    Yuni Retnowati

    2016-04-01

    Full Text Available At first, marketing practitioners and academics consider standardized approaches to marketing and advertising strategies in globalization, and then some studies proved that the standardization of advertising across culture is not valid. Therefore, cross cultural advertising takes local culture into account when conveying messages in advertisements. Cross cultural understanding is very important in order to produce successful localized advertising that would reflect the cultural values and norms of intended audience. Challenge in cross cultural advertising is the problem of communicating to people of diverse cultural background. Cross cultural solutions are applied in areas such as language, communication style, images and cultural values. Cross cultural advertising is simply about using common sense and analyzing how the different elements of an advertising campaign are impacted by culture and modifying them to best speak to the target audience. Other challenges are determining between standardization and adaptation of cultural values content of advertising when facing different people from diverse cultures. In academic side, the challenge is preparing students to design advertisements that communicate effectively to diverse cultures.

  16. Safety culture

    International Nuclear Information System (INIS)

    Keen, L.J.

    2003-01-01

    Safety culture has become a topic of increasing interest for industry and regulators as issues are raised on safety problems around the world. The keys to safety culture are organizational effectiveness, effective communications, organizational learning, and a culture that encourages the identification and resolution of safety issues. The necessity of a strong safety culture places an onus on all of us to continually question whether the safety measures already in place are sufficient, and are being applied. (author)

  17. Radiation produced biomaterials

    International Nuclear Information System (INIS)

    Rosiak, J.M.

    1998-01-01

    radiation technique. Immobilization of biologically active species in hydrogel matrices, their use as drug delivery systems and enzyme traps as well as modification of material surfaces to improve their biocompatibility and ability to bond antigens and antibodies have been the main subject of their investigations. The rising interest in the field of application of radiation to bioengineering was also recognized by the International Atoimc Energy Agency, which has initiated the international programs relating to those studies. In these lectures some directions of investigations on the formation of hydrogels and their applications for biomedical purposes have been specified. Also, some examples of commercialized products being produced by means of radiation technique have been presented

  18. Mean nuclear volume

    DEFF Research Database (Denmark)

    Mogensen, O.; Sørensen, Flemming Brandt; Bichel, P.

    1999-01-01

    We evaluated the following nine parameters with respect to their prognostic value in females with endometrial cancer: four stereologic parameters [mean nuclear volume (MNV), nuclear volume fraction, nuclear index and mitotic index], the immunohistochemical expression of cancer antigen (CA125...

  19. Meaning in couples relationships.

    Directory of Open Access Journals (Sweden)

    Rodrigues T.F.

    2014-09-01

    Full Text Available Based on psycholinguistics and L. Vygotsky’s (2007 theories on sign, meaning and sense categories, as later discussed by A. Leontiev (2004, 2009, we present a case study that focuses on the intricacies of a love relationship for a woman who remained in a painful marriage. Interview material is presented in a Relational-Historical Psychology theoretical framework to provide central categories of meaning and sense. This is understood as a privileged method for apprehending the uniqueness of a human being. To segment the qualitative material, we used the “Analysis of the Nuclei of Meanings for the Apprehension of the Constitution of Sense,” by Aguiar and Ozella (2006, 2013. This approach seeks to discriminate the meanings and senses that constitute the content of a speech sample.

  20. Soils - Mean Permeability

    Data.gov (United States)

    Kansas Data Access and Support Center — This digital spatial data set provides information on the magnitude and spatial pattern of depth-weighted, mean soil permeability throughout the State of Kansas. The...

  1. Beyond Culture.

    Science.gov (United States)

    Barron, Daniel D.

    1993-01-01

    Discusses the lack of literature relating to cultural differences and school library media programs and reviews the book "Beyond Culture" by Edward T. Hall. Highlights include the population/environment crisis, cultural literacy, the use of technology, and Marshall McLuhan's idea of the global village. (LRW)

  2. Bile culture

    Science.gov (United States)

    Culture - bile ... is placed in a special dish called a culture medium to see if bacteria, viruses, or fungi ... Chernecky CC, Berger BJ. Body fluid - anaerobic culture. In: ... . 6th ed. St Louis, MO: Elsevier Saunders; 2013:225-226. Kim AY, ...

  3. Handling Culture

    NARCIS (Netherlands)

    Pieter van Nispen tot Pannerden

    2011-01-01

    The article indicates how companies may prepare for and deal with cultural differences. Because the research base is still rather limited an overall perspective may not be realised. After discussing definitions and concepts of culture, as well as values, cultural differences between states are

  4. Producing charcoal from wastes

    Energy Technology Data Exchange (ETDEWEB)

    Pogorelov, V.A.

    1983-01-01

    Experimental works to use wood wastes for producing charcoal are examined, which are being conducted in the Sverdlovsk assembly and adjustment administration of Soyuzorglestekhmontazh. A wasteless prototype installation for producing fine charcoal is described, along with its subsequent briqueting, which is made on the basis of units which are series produced by the factories of the country. The installation includes subassemblies for preparing and drying the raw material and for producing the charcoal briquets. In the opinion of specialists, the charcoal produced from the wastes may be effectively used in ferrous and nonferrous metallurgy and in the production of pipes.

  5. Mean Profiles of the NEO Personality Inventory

    Czech Academy of Sciences Publication Activity Database

    Allik, J.; Church, A.T.; Ortiz, F. A.; Rossier, J.; Hřebíčková, Martina; De Fruyt, F.; Realo, A.; McCrae, R. R.

    2017-01-01

    Roč. 48, č. 3 (2017), s. 402-420 ISSN 0022-0221 Institutional support: RVO:68081740 Keywords : personality * five-factor model * cross-cultural research * traits * country mean scores * NEO-PI-R * NEO-PI-3 Subject RIV: AN - Psychology OBOR OECD: Psychology (including human - machine relations) Impact factor: 1.657, year: 2016

  6. Vygotsky's Analysis of Children's Meaning Making Processes

    Science.gov (United States)

    Mahn, Holbrook

    2012-01-01

    Vygotsky's work is extensive and covers many aspects of the development of children's meaning-making processes in social and cultural contexts. However, his main focus is on the examination of the unification of speaking and thinking processes. His investigation centers on the analysis of the entity created by this unification--an internal…

  7. Meaning in Matter

    DEFF Research Database (Denmark)

    Brix, Anders

    2013-01-01

    When addressing present-day challenges, design discourse put faith mainly in design understood as a problem-solving activity, pertaining to innovation on functional, systems and technological level. The aesthetically founded fields, e.g. craft-based design, on the other hand, do not seem to play ...... - in particular those which carry _corporeal affordance_, such as clothing, blankets, chairs, tables and houses - perhaps even cities. Things that mean to us, what they mean, mainly _qua_ their physical properties....

  8. Stats means business

    CERN Document Server

    Buglear, John

    2010-01-01

    Stats Means Business is an introductory textbook written for Business, Hospitality and Tourism students who take modules on Statistics or Quantitative research methods. Recognising that most users of this book will have limited if any grounding in the subject, this book minimises technical language, provides clear definition of key terms, and gives emphasis to interpretation rather than technique.Stats Means Business enables readers to:appreciate the importance of statistical analysis in business, hospitality and tourism understand statis

  9. Peer Reviews: a voluntary means of enhancing operating culture

    International Nuclear Information System (INIS)

    Vella, R.

    1996-01-01

    In the nuclear energy industry, and also in other fields, excellent experience has been gained from the regular accumulation of expert evidence of power station operation through external organizations or teams. In 1991, the World Association of Nuclear Operations (WANO) initiated a pilot programme for voluntary 'Peer Reviews'. The success of this first exercise in gathering expert evidence led to the introduction of the 'Peer Review' programme in 1993 as one of the standard programmes of WANO. The object was to increase the safety and reliability of nuclear power stations throughout the world. WANO Peer Reviews are voluntary and are carried out at the request of a member. They are oriented towards special WANO performance objectives and criteria, which have been established by the management of WANO. (orig.) [de

  10. THE MEANING OF HOME IN YORUBA CULTURE OKEYINKA, Y ...

    African Journals Online (AJOL)

    Osondu

    2012-10-03

    Oct 3, 2012 ... relationship between private nature of conveniences in houses which directly influence comfort and privacy and .... The qualitative aspect involved conducting an in- .... subject matter, with an emphasis on the narrative form.

  11. Personal and Cultural Perspectives on Rape and it's Meanings

    DEFF Research Database (Denmark)

    Pedersen, Bodil Maria

    2007-01-01

    Overgreb kan få mange forskellige personlige og kulturelle betydninger. Da der er en tendens til at generalisere overgrebs betydninger, er dette dog et meget ringe udforsket område. Et forskningsprojekt udforsker i samarbejde med Rigshospitalets Center for Voldtægtsofre de betydninger 40 kvinder ...

  12. Iberian Spanish "Macho": Vantages and Polysemy in Culturally Defined Meaning

    Science.gov (United States)

    Grace, Caroline A.; Glaz, Adam

    2010-01-01

    This study explores some specific aspects of compatibility between cognitive models. Robert E. MacLaury's theory of vantages as arrangements of coordinates and Lakoff's concept of radial categories are mutually reinforcing to an analysis of semantic polysemy. Vantage Theory (VT) includes the notions of "zooming in" and "zooming out", allowing…

  13. Cultural Aspects of Suicide

    Directory of Open Access Journals (Sweden)

    Hari D. Maharajh

    2005-01-01

    Full Text Available Undefined cultural factors cannot be dismissed and significantly contribute to the worldwide incidence of death by suicide. Culture is an all embracing term and defines the relationship of an individual to his environment. This study seeks to investigate the effect of culture on suicide both regionally and internationally. Culture-bound syndrome with suicidal behaviours specific to a particular culture or geographical region are discussed. Opinions are divided as to the status of religious martyrs. The law itself is silent on many aspects of suicidal behaviour and despite decriminalization of suicide as self-murder, the latter remains on the statutes of many developing countries. The Caribbean region is of concern due to its steady rise in mean suicide rate, especially in Trinidad and Tobago where socio-cultural factors are instrumental in influencing suicidal behaviour. These include transgenerational cultural conflicts, psycho-social problems, media exposure, unemployment, social distress, religion and family structure. The methods used are attributed to accessibility and lethality. Ingestion of poisonous substances is most popular followed by hanging. The gender differences seen with regard to suicidality can also be attributed to gender related psychopathology and psychosocial differences in help-seeking behaviour. These are influenced by the cultural environment to which the individual is exposed. Culture provides coping strategies to individuals; as civilization advances many of these coping mechanisms are lost unclothing the genetic predisposition of vulnerable groups. In the management of suicidal behaviour, a system of therapeutic re-culturation is needed with an emphasis on relevant culture- based therapies.

  14. Storytelling Adds Meaning

    Science.gov (United States)

    Selby, Victor M.

    2009-01-01

    This article describes several enrichment activities that connect mathematics to science in an algebra 1 curriculum. It provides a basis and suggestions for teachers to include student-produced essays about the role of mathematics in the history of civilization. (Contains 6 figures and 1 table.)

  15. Cultural based preconceptions in aesthetic experience of architecture

    Directory of Open Access Journals (Sweden)

    Stevanović Vladimir

    2011-01-01

    Full Text Available On a broader scale, the aim of this paper is to examine theoretically the effects a cultural context has on the aesthetic experience of images existing in perceived reality. Minimalism in architecture, as direct subject of research, is a field of particularities in which we observe functioning of this correlation. Through the experiment with the similarity phenomenon, the paper follows specific manifestations of general formal principles and variability of meaning of minimalism in architecture in limited areas of cultural backgrounds of Serbia and Japan. The goal of the comparative analysis of the examples presented is to indicate the conditions that may lead to a possibly different aesthetic experience in two different cultural contexts. Attribution of different meanings to similar formal visual language of architecture raises questions concerning the system of values, which produces these meanings in their cultural and historical perspectives. The establishment of values can also be affected by preconceptions resulting from association of perceived similarities. Are the preconceptions in aesthetic reception of architecture conditionally affected by pragmatic needs, symbolic archetypes, cultural metaphors based on tradition or ideologically constructed dogmas? Confronting philosophical postulates of the Western and Eastern traditions with the transculturality theory of Wolfgang Welsch, the answers may become more available.

  16. [Safety culture: definition, models and design].

    Science.gov (United States)

    Pfaff, Holger; Hammer, Antje; Ernstmann, Nicole; Kowalski, Christoph; Ommen, Oliver

    2009-01-01

    Safety culture is a multi-dimensional phenomenon. Safety culture of a healthcare organization is high if it has a common stock in knowledge, values and symbols in regard to patients' safety. The article intends to define safety culture in the first step and, in the second step, demonstrate the effects of safety culture. We present the model of safety behaviour and show how safety culture can affect behaviour and produce safe behaviour. In the third step we will look at the causes of safety culture and present the safety-culture-model. The main hypothesis of this model is that the safety culture of a healthcare organization strongly depends on its communication culture and its social capital. Finally, we will investigate how the safety culture of a healthcare organization can be improved. Based on the safety culture model six measures to improve safety culture will be presented.

  17. Vyryvajas' iz objatij politiki. Issledovanie kul'tur vospominanija kak sposob pooshhrenija istoricheskogo dialoga v litovsko-rossijskih otnoshenijah [An escape from the embrace of politics. A study into the cultures of remembrance as a means to encourage a historical dialogue in Lithuanian-Russian relations

    Directory of Open Access Journals (Sweden)

    Safronovas Vasilijus

    2012-01-01

    Full Text Available This article focuses on the factors inhibiting a productive rethinking of the past in contemporary Lithuanian-Russian relations. The rethinking of the past is understood as a process facilitating the reconsideration of historical meanings through adjusting them to the expectations and values of the contemporary society. The author argues that in this process historical research — as an important tool for encouraging the co-existence of communities —should fulfil certain social functions. Having chosen the Lithuanian-Russian communication space as a case, the author emphasizes the need to develop an interest in mutual knowledge of cultures of remembrance in this space. Outlining the strategies specific to the prevailing cultures of remembrance in contemporary Russia and Lithuania, the author addresses the issue of their proper relationship, as well as that of the appropriateness of historical policy. The criminalisation of certain evaluations of the past and the academic dialogue between historians are regarded as two opposite extremes of historical policy actually existing in the Lithuanian-Russian communication space. The author stresses that research into the values and interpretations of the past peculiar to certain social groups in both Lithuania and Russia may be considered as a basis for further development of the historical dialogue. It is assumed that such research may contribute to the improvement in the field of politics of history.

  18. MEAN STACK WEB DEVELOPMENT

    OpenAIRE

    Le Thanh, Nghi

    2017-01-01

    The aim of the thesis is to provide a universal website using JavaScript as the main programming language. It also shows the basic parts anyone need to create a web application. The thesis creates a simple CMS using MEAN stack. MEAN is a collection of JavaScript based technologies used to develop web application. It is an acronym for MongoDB, Express, AngularJS and Node.js. It also allows non-technical users to easily update and manage a website’s content. But the application also lets o...

  19. Intersubjective meaning making

    DEFF Research Database (Denmark)

    Davidsen, Jacob

    of single-touch screen interaction among 8-9 year-old children presented here, shows that while the constraints of single-touch screens does not support equality of interaction at the verbal and the physical level, there seems to be an intersubjective learning outcome. More precisely, the constraints...... of single-touch screens offer support for intersubjective meaning making in its ability of constraining the interaction. By presenting a short embodied interaction analysis of 22 seconds of collaboration, I illustrate how an embodied interaction perspective on intersubjective meaning making can tell...... a different story about touch-screen supported collaborative learning....

  20. Computing meaning v.4

    CERN Document Server

    Bunt, Harry; Pulman, Stephen

    2013-01-01

    This book is a collection of papers by leading researchers in computational semantics. It presents a state-of-the-art overview of recent and current research in computational semantics, including descriptions of new methods for constructing and improving resources for semantic computation, such as WordNet, VerbNet, and semantically annotated corpora. It also presents new statistical methods in semantic computation, such as the application of distributional semantics in the compositional calculation of sentence meanings. Computing the meaning of sentences, texts, and spoken or texted dialogue i

  1. Biotransformations with plant tissue cultures.

    Science.gov (United States)

    Carew, D P; Bainbridge, T

    1976-01-01

    Suspension cultures of Catharanthus roseus, Apocynum cannabinum and Conium maculatum were examined for their capacity to transform aniline, anisole, acetanilide, benzoic acid and coumarin. None of the cultures transformed acetanilide but each produced acetanilide when fed aniline. All three cultures converted benzoic acid to its para-hydroxy derivative. Coumarin was selectively hydroxylated at the 7-position by Catharanthus and Conium and anisole was O-demethylated only by older Catharanthus tissue.

  2. Recognizing Cultural Differences on Food

    OpenAIRE

    Anacleto, Junia c

    2013-01-01

    Cultural differences play a very important role in matching ICT in- teraction to the expectations of users from different national and cultural back- grounds. But to date, there has been few research as to the extent of such differ- ences, and how to produce software that takes into account these differences. Considering the third wave of HCI research on context, involving the intangible aspects of the interaction with users and ICT solutions, like culture, we are studying these issues using ...

  3. Breastfeeding Education: disagreement of meanings

    Directory of Open Access Journals (Sweden)

    Nydia Stella Caicedo Martínez

    Full Text Available Objective.This work sought to analyze how educational processes have been developed for breastfeeding in a health institution, starting from the meanings mothers, families, and health staff construct thereon. Methods. This was qualitative research of ethnographic approach, which included observations during the group educational activities of the programs, focal groups, and interviews of mothers, their families, and the health staff of a hospital unit in the city of Medellín, Colombia. The analysis was guided by the constant comparison method. Results. The categories emerging from the data were: 1 breast milk is an ideal food. 2 The mothers' experiences influence upon the breastfeeding practice. 3 Family beliefs sometimes operate as cultural barriers. 4 Disagreements are revealed in the educational process. Conclusion. The way educational processes have taken place for breastfeeding reveals a break expressed by the scarce interaction between the meanings professionals have constructed on the topic and those the mothers and their families give to the experience of breastfeeding.

  4. Isolation and morphological characterization of antibiotic producing ...

    African Journals Online (AJOL)

    Purpose: To isolate and characterize antibiotic producing actinomycetes from soil samples in Belgaum, Karnataka, India. Methods: Crowded plate technique was used for the isolation of actinomycetes in media such as soybean – casein digest medium and actinomycetes isolation agar. The morphological and cultural ...

  5. Structural properties of the red-color overglazes on the Kakiemon-style porcelains produced in the later 17th century by means of X-ray diffraction (I Propriedades estruturais, por difração de raios X, de esmaltes vermelhos de porcelanas do estilo Kakiemon produzidos no fim do século 17 (I

    Directory of Open Access Journals (Sweden)

    M. Hidaka

    2009-06-01

    Full Text Available Kakiemon-style porcelains produced at Arita areas (SAGA in Kyushu Island are famous Japanese porcelains. The porcelain-techniques creating its elegant and bright red-color underglaze and overglaze were found and developed in 1650's (early Edo period first by Kakiemon kiln. Red-color overglaze and transparent glaze of the Kakiemon-style porcelains have been investigated by means of X-ray diffraction using synchrotron radiation. The results suggest that the red-color brightness is mainly induced by micro-structural correlation between α-Fe2O3 fine particles, as red-color emission elements, and other oxides of SiO2, Al2O3, CaO, KNaO, PbO. The stability of the red-overglaze on the porcelain surface is related to interfacial fusion of the glasses existing in the fritted red-overglaze and the transparent glaze on the porcelain body. The ancient porcelain-techniques of the Kakiemon-style porcelains are clearly based on the micro-structural and material properties of the overglazes, the underglazes, and the transparent glazes, though the techniques were experimentally and accidentally found and developed in the Edo period.As porcelanas do estilo Kakiemon produzidas nas áreas de Arita (SAGA na ilha Kyushu são porcelanas japonesas famosas. As técnicas de produzir porcelanas com os elegantes e brilhantes vidrados de cores vermelho brilhante foram encontradas e desenvolvidas nos anos 1650 (início do período Edo primeiramente em fornos Kakiemon. Vidrados vermelhos e vidrados transparentes de porcelanas do estilo Kakiemon foram investigadas por meio de difração de raios X com radiação sincrotron. Os resultados sugerem que o brilho de cor vermelha é principalmente induzido pela correlação microestrutural entre finas partículas de α-Fe2O3, como elementos emissores de cor vermelha, além de outros óxidos como SiO2, Al2O3, CaO, KNaO, e PbO. A estabilidade dos vidrados na superfície da porcelana com vidrado vermelho está relacionada com a fus

  6. Engagement Means Everyone

    Science.gov (United States)

    Patton, Carol

    2012-01-01

    Employee engagement is not just HR's responsibility. While HR is responsible for the process of measuring and driving engagement, improving it is actually everyone's responsibility. And that means reducing the barriers to productivity to drive business performance. Training departments can play a pivotal role. Their job is to enhance curriculum or…

  7. Monstrosity by Monstrous Means

    DEFF Research Database (Denmark)

    Post, Hans Christian

    2016-01-01

    The Cambodian memorials to the Cambodian genocide between 1975 and 1979 can in numerous ways be termed monstrous. Not so much in that they deal with atrocities monstrous in scale and character, as in the monstrous visual means they employ and the imprecise, monster-ridden explanations they delive...

  8. The meaning of talent

    Directory of Open Access Journals (Sweden)

    Raquel Lorenzo

    2006-01-01

    Full Text Available The knowledge society gives a remarkable role to human potentialities into organizations. For this reason has emerged talent management. But it is necessary to know its meaning to put on practice programs. In this paper, the author analyzes different points of views about this topic.

  9. PR Means Positive Results.

    Science.gov (United States)

    Foree, Marilyn

    1992-01-01

    Describes the advantages of public relations to a school music program. Suggests that, to succeed, a music program must have the support of school administrators, teachers, parents, and students alike. Recommends means of attracting support from the four groups. Stresses the importance of effective local publicity. (SG)

  10. Jeans and Means

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 1; Issue 8. Jeans and Means - The Story of Indigo. Subramania Ranganathan. Series Article Volume 1 Issue 8 August 1996 pp 22-27. Fulltext. Click here to view fulltext PDF. Permanent link: https://www.ias.ac.in/article/fulltext/reso/001/08/0022-0027 ...

  11. Levels of Literary Meaning

    DEFF Research Database (Denmark)

    Klausen, Søren Harnow

    2017-01-01

    I argue that intentionalist theories of meaning and interpretation, like those of Hirsch and Juhl, have been insufficiently attentive to the different levels of authorial intention that are operative in literary works. By countenancing intentions on different levels – ranging from simple semantic...

  12. Cultural Aspects in the Shona Monolingual Dictionary:

    African Journals Online (AJOL)

    Riette Ruthven

    All this knowledge is acquired socially through interaction. ... about the meaning of a word without recognising the wider social and cultural reality of ..... tics. Harmondsworth: Penguin Books. Goodenough, W.H. 1957. Cultural Anthropology and ...

  13. Biologically produced sulfur

    NARCIS (Netherlands)

    Kleinjan, W.E.; Keizer, de A.; Janssen, A.J.H.

    2003-01-01

    Sulfur compound oxidizing bacteria produce sulfur as an intermediate in the oxidation of hydrogen sulfide to sulfate. Sulfur produced by these microorganisms can be stored in sulfur globules, located either inside or outside the cell. Excreted sulfur globules are colloidal particles which are

  14. Consumers and Producers

    NARCIS (Netherlands)

    E. Maira (Elisa)

    2018-01-01

    markdownabstractIn the last few decades, advances in information and communication technology have dramatically changed the way consumers and producers interact in the marketplace. The Internet and social media have torn down the information barrier between producers and consumers, leading to

  15. Producers and oil markets

    International Nuclear Information System (INIS)

    Greaves, W.

    1993-01-01

    This article attempts an assessment of the potential use of futures by the Middle East oil producers. It focuses on Saudi Arabia since the sheer size of Saudi Arabian sales poses problems, but the basic issues discussed are similar for the other Middle East producers. (Author)

  16. Identify Web-page Content meaning using Knowledge based System for Dual Meaning Words

    OpenAIRE

    Sinha, Sukanta; Dattagupta, Rana; Mukhopadhyay, Debajyoti

    2012-01-01

    Meaning of Web-page content plays a big role while produced a search result from a search engine. Most of the cases Web-page meaning stored in title or meta-tag area but those meanings do not always match with Web-page content. To overcome this situation we need to go through the Web-page content to identify the Web-page meaning. In such cases, where Webpage content holds dual meaning words that time it is really difficult to identify the meaning of the Web-page. In this paper, we are introdu...

  17. Spatial Culture

    DEFF Research Database (Denmark)

    Reeh, Henrik

    2012-01-01

    Spatial Culture – A Humanities Perspective Abstract of introductory essay by Henrik Reeh Secured by alliances between socio-political development and cultural practices, a new field of humanistic studies in spatial culture has developed since the 1990s. To focus on links between urban culture...... and modern society is, however, an intellectual practice which has a much longer history. Already in the 1980s, the debate on the modern and the postmodern cited Paris and Los Angeles as spatio-cultural illustrations of these major philosophical concepts. Earlier, in the history of critical studies, the work...... Foucault considered a constitutive feature of 20th-century thinking and one that continues to occupy intellectual and cultural debates in the third millennium. A conceptual framework is, nevertheless, necessary, if the humanities are to adequa-tely address city and space – themes that have long been...

  18. 7 CFR 1124.13 - Producer milk.

    Science.gov (United States)

    2010-01-01

    ... section, Producer milk means the skim milk (or skim milk equivalent of components of skim milk), including... filed a request in writing with the market administrator before the first day of the month the agreement... producer deliveries of each according to a method approved by the market administrator. (4) Diverted milk...

  19. Objects, materiality and meaning

    DEFF Research Database (Denmark)

    Lenau, Torben Anker; Lindegaard, Hanne

    2008-01-01

    The present research work investigates the relation between physical objects, their materiality, understood as the physical substances they are made from, and the communication from the objects. In product design of physical objects the communicative aspects are just as important as the function...... of the object, and the designers aim is therefore to tune both in order to achieve a desired goal. To do so the designer basically has 2 options: Alteration of the physical shape of the object and the selection of materials. Through the manipulation of shape and materials can symbolic and sensory information...... be written into the object. The materials are therefore carriers of communication, even though this is dependent of the cultural context and the environment which the object will be part of. However the designer has only minor influence on those....

  20. Cultural contrast

    Institute of Scientific and Technical Information of China (English)

    周志

    2016-01-01

    Chinese cultural contains a great number of styles;culture differentiation does not depend on region differentiation.This research would interpret what difference between Hong Kong and Shenzhen.1.Food culture in china Traditional Chinese medicine suggests eating local seasonal fruit and vegetables,as they are most suitable for the body during a particular season.It is also divided food into 3 characteristics:cooling foods,warming foods and balance or