Sample records for culturally produced meanings

  1. Music, Meaning and Culture

    Richard Widdess


    Full Text Available This paper situates musical meaning in culture, addressing music as social symbol and as ongoing process of meaning creation. Three examples of non- Western musical practice are used to illustrate the embedding of musical meaning in cultural context. The performance of an Australian Aboriginal song is shown to exemplify the interdependence of song style and social structure as a matrix for the emergence of cultural meanings; an example of North Indian performance is adduced to demonstrate the multi-layered nature of meaning as embodied in musical performance; and an example of collective festival performance from Nepal illustrates ways in which the structure of musical performance can mirror local cultural forms. Each of the three examples lends weight to the idea that music's meanings are often non-linguistic and reflect foundational schemas that are specific to the cultures from the musics are drawn.

  2. When means become ends: technology producing values

    Bjørn Hofmann


    Full Text Available Technology has become the symbol of our culture. The claim that we are subject to a technological imperative is therefore a fundamental cultural critique: we do not control technology, rather technology controls us. An alternative way to formulate this is to claim that technology cannot be “made down” when it is made up; we just have to make the best of it. Accordingly, it has been argued that technology has evolved from being merely a means to becoming an end in itself. This article investigates this claim by analyzing the relationship between technology and values. The examples stem from the technologies of medicine and weapons because they clarify this relationship. It is argued that technology relates to values in two ways. Technology both raises general questions about values and it is value-laden due to its very function. However, although technology is value-laden, it does not necessarily give an imperative mandate. One reason for this lies in our responsibility. We are inevitably responsible for all aspects of technology, i.e. development, construction, production, commercialization, implementation, and use. Referring to a technological imperative to explain and defend our decisions with respect to technology constitutes an unjustified renunciation of our own responsibility. Hence, the article tries to underscore our responsibility by developing a technological axiology.

  3. Social and Cultural Influence on Word Meaning



    Different nations have different social cultures, and the social culture of every nation is developing with the devel-opment of the nation, language is greatly influenced by social culture, which enables language to possess national and historical characteristics. Word is the smallest, independent, meaningful linguistic unit of language. It can well reflect the national and historical characteristics. From synchronic aspect, the social and cultural influence on word meaning is mainly embodied in con-ceptual and associative meanings of word. The incorrespondence of word meaning is a phenomenon that exists in both conceptual and associative meanings of word among different languages. From diachronic aspect, the development of social culture causes some changes of word meaning to some extent. Therefore, social culture plays a key role in vocabulary learning and mastery of a language.

  4. Evaluative meaning and its cultural significance

    Drazdauskiene, Maria Liudvika


    In the framework of traditional descriptive semantics, evaluative meaning is defined as an aspect of affective meaning. By virtue of its general positive and negative evaluation, evaluative meaning finds its place in the compartment of interpersonal meaning in functional linguistics. The concept of evaluative meaning is also in agreement with the categorisation of meaning in contemporary stylistics. Having stated its spread and disagreement with logic, the cultural significance of...

  5. Musics, Cultures and Meanings: Music as Communication

    Ian Cross


    Full Text Available This commentary explores interpretations of concepts that lie at the focus of Richard Widdess's paper—"music", and "culture"—with the aim of specifying frameworks within which issues of musical meaning can fruitfully be addressed.

  6. Confronting mortality: faith and meaning across cultures.

    Paulson, Steve; Kellehear, Allan; Kripal, Jeffrey J; Leary, Lani


    Despite advances in technology and medicine, death itself remains an immutable certainty. Indeed, the acceptance and understanding of our mortality are among the enduring metaphysical challenges that have confronted human beings from the beginning of time. How have we sought to cope with the inevitability of our mortality? How do various cultural and social representations of mortality shape and influence the way in which we understand and approach death? To what extent do personal beliefs and convictions about the meaning of life or the notion of an afterlife affect how we perceive and experience the process of death and dying? Steve Paulson, executive producer and host of To the Best of Our Knowledge, moderated a discussion on death, dying, and what lies beyond that included psychologist Lani Leary, professor of philosophy and religion Jeffrey J. Kripal, and sociologist Allan Kellehear. The following is an edited transcript of the discussion that occurred February 5, 7:00-8:30 pm, at the New York Academy of Sciences in New York City.

  7. Organ culture system as a means to detect celiac disease.

    Picarelli, Antonio; Libanori, Valerio; De Nitto, Daniela; Saponara, Annarita; Di Tola, Marco; Donato, Giuseppe


    Anti-endomysial and anti-transglutaminase antibodies can be produced in vitro by the intestinal mucosa of celiac disease (CD) patients in clinical remission, when the culture is performed in the presence of gliadin peptides. Our aim was to use this organ culture system as a means to detect the pathognomonic antibodies of celiac disease (CD) in the culture supernatants. Organ culture was performed in the presence of three different activators to evaluate which one induced the strongest antibody response in intestinal mucosa from patients in clinical remission of CD. Our data confirm the high efficiency of synthetic peptide 31-43 as a specific immunological activator in CD and demonstrate its capability to stimulate production/secretion of CD-specific antibodies. We envision that this organ culture system may prove to be useful as a new technique for CD diagnosis.

  8. Flows of Meaning, Cultures of Movements

    Jensen, Ole B.


    Contemporary cities and places are defined by mobility and flows as much as by their sedentary and fixed properties. In the words of Shane the city may be seen as configured by ‘enclaves' (fixed and bounded sites) and ‘armatures' (infrastructure channels and transit spaces). By taking point of de...... production. The outcome is a theoretical argument for the exploration of the potentials of armature spaces in order to point at the importance of ‘ordinary' urban mobility in creating flows of meaning and cultures of movement.......Contemporary cities and places are defined by mobility and flows as much as by their sedentary and fixed properties. In the words of Shane the city may be seen as configured by ‘enclaves' (fixed and bounded sites) and ‘armatures' (infrastructure channels and transit spaces). By taking point...... of departure in a critique of such a sedentary / nomad dichotomy the paper aims to move beyond these arguing for a third position of ‘critical mobility thinking'. The theoretical underpinning of this position reaches across cultural theory, human geography and into sociology. It includes a notion...

  9. Different Symbolic Meaning of Color in Chinese and Western Cultures

    钟维维; 裘瑜; 陈佳颖


    Colors are embodied with different meanings and symbols in different countries. Not only should we understand their basic and literal meanings, but also we should focus on their deep contents in symbolic meanings, because their symbolic meanings vary from culture to culture. The differences in the symbol of colors are due to different cultures and history back-grounds as well as aesthetic psychology. This paper, mainly based on the Chinese and western culture, approaches the symbols of colors represented in the two different cultures through analyzing the different understanding of colors, which can help us under-stand culture difference in colors better during intercultural com-munication.

  10. Cultural Differences and the Construction of Meaning

    Robert A. Peña


    Full Text Available The relationships between student achievement, student culture and practitioners' attitudes and expectations were investigated. Student achievement was defined as academic performance but also included perceptions, rationales and explanations for student behaviors and conduct. Student culture described student's Mexican American origins, customs and beliefs. Practitioners' attitudes described how middle school personnel perceived Mexican American high and underachieving students generally, and practitioners' expectations described how personnel interacted and behaved toward Mexican American students. Results indicated that Mexican American students perceived themselves and school personnel perceived these students as different from Anglo students. Mexican American cultural traditions were also perceived as inferior and disadvantageous by high achieving Mexican American students and by personnel. Underachieving Mexican American students generally valued their cultural traditions more positively than high achieving students becoming resistant to learning when these traditions were marginalized in school. Student achievement was also related to student compliance, student appearance, styles in written and verbal communication and practitioners' perceptions about the willingness of Mexican American students to practice and support Anglo norms. These findings are congruent with theories that discuss relationships between student achievement, student culture and practitioners' attitudes and expectations. Theories about school failure occurring less frequently in minority groups that are positively oriented toward their own and the dominant culture were contradicted and not supported in this research.

  11. Cultural Meanings Construction: An Analysis of the Organic Grape Juice Market

    Marlon Dalmoro


    Full Text Available Regarding the meanings networks that involve markets, this research aims to analyze cultural meanings production in the organic grape juice market, involving consumers and producers agents. It was adopted a qualitative approach with an interpretative character by interviews with 25 consumers and producers. Cultural meanings construction description and analysis involved the socio-cultural context, interaction between agents (producers and consumers and meanings assigned by each agent. Organics are meant as food for both agents. However, others meanings associated to organics operate in dichotomic levels. It results in a non-homogeneous network between producers and consumers, reflecting the different cultural universe of each agent. These results mainly contribute in understanding the patterns of production and consumption around the food market. The lack of consolidated networks of meanings limit the construction and establishment of organic products market structures.



    Key words: Meaning, House, Home, Yoruba culture, Cradle, Premium ... examining the social, psychological, cultural and physical .... scheme. This is used in the textual narrative of the subject matter, with an emphasis on the narrative form.

  13. Cultural Context and Associative Meaning of Words in Cross-culture Communication

    吴玉侠; 董艇舰


    Language is the vehicle of culture, vocabulary is the most active part of a language. This article compares and analyses the relationship of cultural context and the associative meaning of words in three aspects—associative meaning overlap, associative meaning mismatch and associative meaning gap, revealing its importance in the cross-cultural communication.

  14. African-American Vernacular Dance: Core Culture and Meaning Operatives.

    Hazzard-Gordon, Katrina


    Discusses the role of social dancing in the lives and culture of working class Black Americans. Focuses specifically on four aspects of its meaning: identity (self-esteem), cultural integrity, ingroup-outgroup, and political resistance. Bases argument on sociological, biographical, and fictional works by and about Black culture. (KH)

  15. Greater learnability is not sufficient to produce cultural universals.

    Rafferty, Anna N; Griffiths, Thomas L; Ettlinger, Marc


    Looking across human societies reveals regularities in the languages that people speak and the concepts that they use. One explanation that has been proposed for these "cultural universals" is differences in the ease with which people learn particular languages and concepts. A difference in learnability means that languages and concepts possessing a particular property are more likely to be accurately transmitted from one generation of learners to the next. Intuitively, this difference could allow languages and concepts that are more learnable to become more prevalent after multiple generations of cultural transmission. If this is the case, the prevalence of languages and concepts with particular properties can be explained simply by demonstrating empirically that they are more learnable. We evaluate this argument using mathematical analysis and behavioral experiments. Specifically, we provide two counter-examples that show how greater learnability need not result in a property becoming prevalent. First, more learnable languages and concepts can nonetheless be less likely to be produced spontaneously as a result of transmission failures. We simulated cultural transmission in the laboratory to show that this can occur for memory of distinctive items: these items are more likely to be remembered, but not generated spontaneously once they have been forgotten. Second, when there are many languages or concepts that lack the more learnable property, sheer numbers can swamp the benefit produced by greater learnability. We demonstrate this using a second series of experiments involving artificial language learning. Both of these counter-examples show that simply finding a learnability bias experimentally is not sufficient to explain why a particular property is prevalent in the languages or concepts used in human societies: explanations for cultural universals based on cultural transmission need to consider the full set of hypotheses a learner could entertain and all of

  16. Consumer Culture Theory: Ideology, Mythology and Meaning in Technology Consumption

    Bajde, Domen


    Consumer culture theory helps us take note of the cultural forces and dynamics in which technology consumption is entangled. It enables people to articulate the cultural processes (ideological, mythic, ritualistic, etc.) through which cultural meanings become granted to or denied to technological...... innovations, thus shaping the value of technologies as cultural resources sustaining consumer identities. In its urge to shed light on these aspects, CCT tends to reinforce the gaps and asymmetries between the “socio-cultural” and the “techno-material”, leaving plenty of room for further study. The authors...

  17. Non-verbal Means in Cross-cultural Communication

    Shara Mazhitaeva


    Full Text Available The article covers the non-verbal means of communication which are significant factors of cross-cultural communication. Authors dwell on the features of kinetic components of speech and confirm the idea that language is universal in its basis and national according to different ways of expression.

  18. What Psychoanalysis, Culture And Society Mean To Me

    Layton, Lynne


    The paper reviews some ways that the social and psychic have been understood in psychoanalysis and argues that a model for understanding the relation between the psychic and the social must account both for the ways that we internalize oppressive norms as well as the ways we resist them. The author proposes that we build our identities in relation to other identities circulating in our culture and that cultural hierarchies of sexism, racism, classism push us to split off part of what it means to be human, thereby creating painful individual and relational repetition compulsions. These “normative unconscious processes” replicate the unjust social norms that cause psychic pain in the first place. The paper concludes with thoughts about contemporary US culture, in which the government has abdicated responsibility toward its most vulnerable citizens and has thus rendered vulnerability and dependence shameful states. PMID:22058628

  19. Immunomodulatory potential of shatavarins produced from Asparagus racemosus tissue cultures.

    Pise, Mashitha Vinod; Rudra, Jaishree Amal; Upadhyay, Avinash


    Medicinal properties of Asparagus racemosus (vernacular name: Shatavari) are attributed to its steroidal saponins called shatavarins. This plant is facing the threat of being endangered due to several developmental, seasonal constrains and malpractices involved in its collection and storage. To support its conservation, a tissue culture protocol is standardized which produces 20 fold higher levels of shatavarin. Here we evaluate the bioactivity and immunomodulatory potential of in vitro produced shatavarins from cell cultures of AR using human peripheral blood lymphocytes. In vitro produced shatavarin stimulated immune cell proliferation and IgG secretion in a dose dependent manner. It stimulated interleukin (IL)-12 production and inhibited production of IL-6. It also had strong modulatory effects on Th1/Th2 cytokine profile, indicating its potential application for immunotherapies where Th1/Th2 balance is envisaged. Our study demonstrating the bioactivity of tissue cultured AR extracts supports further in vivo evaluation of its immunomodulatory efficacy.

  20. Spreadable media: Creating value and meaning in a networked culture

    Moderated by Louisa Stein


    Full Text Available Online Roundtable on Spreadable Media, by Henry Jenkins, Sam Ford, and Joshua Green, with participants Paul Booth, Kristina Busse, Melissa Click, Sam Ford, Henry Jenkins, Xiaochang Li, and Sharon Ross. Section 1 first published as the article "Spreadable Media: Creating Value and Meaning in a Networked Culture", by Louisa Stein, from Cinema Journal Volume 53 Issue 3, pp152-177. Copyright 2014 by The University of Texas Press. All rights reserved.

  1. Nothingness and the human umwelt: a cultural-ecological approach to meaning.

    Bang, Jytte


    In the paper I argue that the great impact of empiricism on psychology and the enclosed dualist agenda traps psychological phenomena into subjectivism. By discussing the phenomena of nothingness in biological and cultural life it is argued that meaning must be considered as a phenomenon that represents both a fit and a misfit of the individual with the environment. By stressing the overall presence of nothingness phenomena it is argued how the reduced ontology of empiricism--and its blindness to relations and transformations out of which meaning grows--should be overcome. In human cultural life, transformations are constitutive and ongoing changes are being produced to make sure that continuity as well as discontinuity will happen. The analysis of especially one case--the removal of an Amish school after a shooting episode--serves to prove how meaning grows out of cultural processes as people produce their own conditions of life. From a cultural-ecological point of view, analyzing meaning at the level of individual phenomenology, hence, means analyzing the 'total psychological situation' (legacy of Kurt Lewin). This may for instance include analyzing how people live, what they consider important and worth preserving, what must be changed, what are their core values and how do institutional arrangements contribute to keeping up that which is valued or to changing that which is not, etc. Meaning may be viewed the lived-out experience-the domain of self-generativity in human life.

  2. Domestically Produced TV-Drama and Cultural Commons

    From, Unni


    The Chapter elaborates empirically some of the aspects and means by which drama can occasion an articulation of cultural commons. Further, it explores explanations why audiences might retain a national perspective as a dominating frame of interpretation even though universal or late-modern interp......The Chapter elaborates empirically some of the aspects and means by which drama can occasion an articulation of cultural commons. Further, it explores explanations why audiences might retain a national perspective as a dominating frame of interpretation even though universal or late...

  3. Domestically Produced TV-Drama and Cultural Commons

    From, Unni


    The Chapter elaborates empirically some of the aspects and means by which drama can occasion an articulation of cultural commons. Further, it explores explanations why audiences might retain a national perspective as a dominating frame of interpretation even though universal or late-modern interp......The Chapter elaborates empirically some of the aspects and means by which drama can occasion an articulation of cultural commons. Further, it explores explanations why audiences might retain a national perspective as a dominating frame of interpretation even though universal or late...

  4. Meanings Of The Notion Of Culture In Intercultural Research

    Angelica-Nicoleta ONEA


    Full Text Available There are many meanings given to the notion of culture. Various contents related to it are delimited according to the specialty field or the investigated area. Moreover, the overlapping or distinction between culture and civilization also occurs. An inventory and an analysis of the aforementioned aspects could be a subject for exhaustive research. We are not aiming at such an approach, our intention is to analyze the philosophies circumscribed to this concept in some of the most important investigations carried out in the field of intercultural management so as to distinguish in a very precise manner the sense given and to focus on the elements that allow us to build a definition with an operational content in this area of research. For this purpose, we also identify the perspectives that allowed us to develop a functionalist-instrumental pragmatic approach.

  5. Determination of Quality Properties in Labneh Produced with Probiotic Culture

    Tuğba Kök Taş


    Full Text Available The recent years, functional food market has been dramatically growing due to the increasing number of conscious consumers. More products in the field of functional foods include dairy products. Diversity of functional foods that are very popular in the world are very limited in Turkey. Use of probiotic microorganisms in dairy products are provided positive contribution to public health. Probiotic products are featured in many studies to affect the intestinal immune system, regulating the immune system. The aim of this reseach was to use probiotic starter culture to improve functional properties of Labne that is a very nutritious dairy product and preferred by children and youths. The quality properties (chemical, microbiological and sensorial of Labneh were determined. pH value and lactic acid of Labneh sample with yogurt culture and Labneh sample produced with Lactobacillus acidophilus culture were determined 4.53 and 4.58; 0.52 and 0.53, respectively. Labneh sample containing probiotic culture had 21% fat and 29.38% total solid. Labneh sample with probiotic culture contained 8.97 log cfu/ml as content of L. acidophilus. Result of sensory analyses showed that Labneh sample produced using L. acidophilus had higher taste, odor and texture scores.

  6. Cultural meanings of musculoskeletal diseases in Chile's Mapuche Population.

    Alarcon, Ana M; Vidal, Aldo; Castro, Marcela


    Eight out of 10 Mapuche indigenous women have a musculoskeletal disorder (MSD) and do not seek early medical aid. To study both the cultural meanings and implications of MSD from the Mapuche worldview. Ethnographic study carried out from 2008 to 2011 on indigenous reserves in southern Chile. Sixty-four Mapuches participated in comprehensive interviews, which were transcribed and analyzed by the research team. Five cultural domains: (a) foro kutran/bone disease is the general denomination of MSD; (b) Re-Rume Kutran/progressive and incurable course, refers to the course of the disease; (c) Kalül fücha mawiza/body is an old tree, describes internal manifestations such as worn bones, dry body, weak blood, and spiritual weakness; (d) witrür tripai foro/deformation is the external manifestation of MSD; and (e) Reñma ka lof kutran/family and community suffering refers to the impact of MSD. The explanation of MSD is consistent with the integrated body-nature-spirit worldview of the Mapuche. To provide cultural nursing health care so that patients receive prompt diagnosis and care.

  7. Culture cultivating culture: the four products of the meaning-made world.

    Carriere, Kevin R


    Culture, in a semiotic cultural psychology, is defined from the viewpoint of cultivation--the meaning making processes that give meaning to the world (Valsiner 2000, 2007a). However, the individual is not simply a process-machine in an empty world--there are both the external outcomes of meaning making (individual and group based) as well as the collective influence on the cultivation process. I argue to examine the cultivation process more completely, one must look at these external influences that catalyze future cultivation processes. By examining the power of the external (environmental Umwelten) and group-internal (myths, morals), a much greater understanding of the behavior of individuals can be accomplished beyond examining the individual's process of meaning making. Further work into examining the objects that affectively activate the individual as well as group action and meaning making is called for and examples of such studies are given.

  8. Cultural meanings of nature: an analysis of contemporary motion pictures.

    Pollio, Howard R; Anderson, John; Levasseur, Priscilla; Thweatt, Michael


    To evaluate current cultural meanings of nature, the authors asked 65 undergraduate students to "list 3 movies in which nature was an important aspect of the film." They also were asked to specify the natural element that stood out to them and describe "how it related to the overall theme of the movie." Two independent groups of raters skilled in interpretive analysis developed thematic meanings from these responses. Following this, in a 2nd study, a different group of participants rated the 18 most frequently mentioned natural elements on thematic scales derived from the initial interpretive analysis. Participants in the 1st study mentioned 33 different movies at least twice and 5 themes that captured the meaning of nature in these films. Correlational results derived from the 2nd study indicated that rating scales reflecting these 5 themes formed 2 distinct groups; the first group described settings in which nature is experienced as adversarial and plays a significant role in dramatic action, and the second group defined settings in which nature is viewed either as a place of refuge or simply as a locale in which ongoing narrative action occurs. The general conclusion reached in both studies concerns the often noted but not always appreciated fact that movies--similar to everyday events and actions--always take place in specific settings and that neither life events nor dramatic narratives can be understood apart from specific settings.

  9. Religion as memory: How has the continuity of tradition produced collective meanings? – Part one

    Jakub Urbaniak


    Full Text Available Danièle Hervieu-Léger gives an account of religion as a chain of memory, that is, a form of collective memory and imagination based on the sanctity of tradition. According to her theory, in the postmodern world the continuity of religious memory has been broken and all that remains are isolated fragments guarded by religious groups. This twofold study aims at showing, firstly, in what sense religion can be conceived of as memory which produces collective meanings (Part One and, secondly, what may happen when individualised and absolutised memories alienate themselves from a continuity of tradition, thus beginning to function as a sort of private religion (Part Two. Being the first part of the study in question, this article is dedicated to a historical-theological analysis of religious memory as a source of collective meanings, as seen from a Christian perspective. Firstly, it situates Hervieu-Léger’s definition of religion against the background of the most topical religious contexts in which the notion of memory appears today. Secondly, the dialectics of individual and collective memory is discussed, notably through the lens of Ricoeur’s original proposal. This is followed by an overview of the traditional functions of memory in Christianity. Lastly, the interpretation of the way in which Christian tradition, in its premodern continuity, served as a source of collective cultural meanings, is recapitulated. What underlies this analysis is the conviction that to comprehend, and even more so to challenge mechanisms based on which the dominant purveyors of meaning (such as economic and information market function in our day, one should have a clear understanding of what they attempt to substitute for. In brief, before exploring how memories become religion, one ought to be able to conceive of religion as memory.

  10. Cultural distance in international business and management : from mean-based to variance-based measures

    Beugelsdijk, Sjoerd; Maseland, Robbert; Onrust, Marjolijn; van Hoorn, Andre; Slangen, Arjen


    Extant practice in international management is to measure cultural distance as a nation-to-nation comparison of country means on cultural values, thereby ignoring the cultural variation that exists within countries. We argue that these traditional mean-based measures of cultural distance should take

  11. Cross-Cultural Understanding for Global Sustainability: Messages and Meanings from Asian Cultural Landscapes

    Singh, R.


    Concept of 'multifunctionality' of cultural landscapes is a reflection of imbued meaning and aesthetics inherent there and also human manifestation of this spirit through existence and aliveness by human creation, love and continuance in various cultures and traditions. This sense helps envisioning landscapes that cross urban-rural divides in sustainable and an integrated way - characterised by wholeness and ecospirituality that developed in the cultural history of landscape sustainability. That is how, the idea of 'wholeness' (cosmality) is transformed into 'holiness' (sacrality) ― evolved and represented with sacred ecology and visualised through the cosmic frames of sacredscapes in Asian region that survived there as part of lifeworld. Understanding, feeling, living with, practicing and passing on these inherent meanings and aesthetics provide peace, solace and deeper feelings to human mind which are the ethereal breathe of sustainability. The rethinking should be based on the foundational value ― the reasoning that underlies the ethical sense of deeper understanding of Man-Nature Interrelatedness, the basic philosophy of coexistence ― referred in different cultures in their own ways, like multicultural co-living ('Old-comer') in Korea, harmonious coexistence (tabunka kyosei) in Japan, harmonious society (xiaokang) in China, wahi tapu (sacred places) in Maori's New Zealand, global family (vasudhaiva kutumbakam) in Indian thought, and also African humanism (ubuntu) in South Africa. Think universally, see globally, behave regionally, act locally but insightfully; this is an appeal for shared wisdom for global sustainability in making our cultural landscapes mosaic of happy, peaceful and sustainable places crossing all the borders and transitions, especially interwoven links among Korea, Japan, China, and India.

  12. Technological Education as a Means of Developing Students' Health Culture

    Masalimova, Alfiya R.; Luchinina, Anastasia O.; Ulengov, Ruslan A.


    The urgency of the research is due to the fact that health of school-age children in Russia is deteriorating. The development of health culture has become an integral part of students' general cultural development. The purpose of this article is to reveal the potential of "Technology" as a school subject for the development of students'…

  13. Meanings and Implications of Culture in Sustainability Education Research

    Anderson, Vince; Datta, Ranjan; Dyck, Shannon; Kayira, Jean; McVittie, Janet


    As scholars working both individually and collectively, we are interested in exploring what may be achieved through taking up the complex notion of culture in sustainability education research. In this article, we present a bricolage of research, drawing on empirical and theoretical sources that collectively establish the kind of capacity we see…

  14. Innovation in Intercultural Contexts: Construction of Meaning from Reference Cultures

    Miranda Ossandón, Jorge; Villagra, Carolina; Sepùlveda, Segundo


    This investigation was carried out in an attempt to respond to the issue of identification of codes for constructing meaning that yield to innovation. Based on the discourse of rural primary school teachers of la Araucania Region, Chile, we sought to expose key elements and codes that allow teachers to construct and/or re-elaborate meaning that…

  15. The Cultural Psychology of Suffering: The Many Meanings of Health in Orissa, India (And Elsewhere)

    Richard A. Shweder


    In this article, I honor Jerome Bruner's meaning-centered and person-centered approach to the study of cultural psychology by describing aspects of the cultural psychology of suffering in and around...

  16. Integrating cultural community psychology: activity settings and the shared meanings of intersubjectivity.

    O'Donnell, Clifford R; Tharp, Roland G


    Cultural and community psychology share a common emphasis on context, yet their leading journals rarely cite each other's articles. Greater integration of the concepts of culture and community within and across their disciplines would enrich and facilitate the viability of cultural community psychology. The contextual theory of activity settings is proposed as one means to integrate the concepts of culture and community in cultural community psychology. Through shared activities, participants develop common experiences that affect their psychological being, including their cognitions, emotions, and behavioral development. The psychological result of these experiences is intersubjectivity. Culture is defined as the shared meanings that people develop through their common historic, linguistic, social, economic, and political experiences. The shared meanings of culture arise through the intersubjectivity developed in activity settings. Cultural community psychology presents formidable epistemological challenges, but overcoming these challenges could contribute to the transformation and advancement of community psychology.

  17. The cultural animal human nature, meaning, and social life

    Baumeister, Roy F


    What makes us human? Why do people think, feel, and act as they do? What is the essence of human nature? What is the basic relationship between the individual and society? These questions have fascinated both great thinkers and ordinary humans for centuries. Now, at last, there is a solid basis for answering them, in the form of the accumulated efforts and studies by thousands of psychology researchers. We no longer have to rely on navel-gazing and speculation to understand why people are the way they are - we can instead turn to solid, objective findings. This book, by an eminent social psychologist at the peak of his career, not only summarizes what we know about people - it also offers a coherent, easy-to-understand, through radical, explanation. Turning conventional wisdom on its head, the author argues that culture shaped human evolution. Contrary to theories that depict the individual's relation to society as one of victimization, endless malleability, or just a square peg in a round hole, he proposes t...

  18. Cultural meanings of tuberculosis in Aceh Province, Sumatra.

    Caprara, A; Abdulkadir, N; Idawani, C; Asmara, H; Lever, P; De Virgilio, G


    This paper shows how disease transmission and particularly what biomedicine calls tuberculosis are interpreted in the non-Western context of Aceh Province, Sumatra, Indonesia. It tries also to focus on factors influencing perceptions and health-seeking behaviors. Results show that what biomedicine calls TB is represented by a semantic network of illnesses. Parts of this network are clearly identified as transmissible while others are related to specific phenomena affecting the individual, such as terbuk (poisoning) or trouk (fatigue produced by hard work), and are not considered contagious. Forms of transmission are interpreted mostly through empirical and analogic categories. TB is attributed to four different aspects: (a) biomedical categories such as germ theory; (b) socio-economic conditions; (c) transgression of social rules; and (d) poisoning and the influences of supernatural powers. Health-seeking behaviors are related to the perceived causes of the disease, economic factors, and the accessibility of health services.

  19. Bicarbonate produced from carbon capture for algae culture.

    Chi, Zhanyou; O'Fallon, James V; Chen, Shulin


    Using captured CO(2) to grow microalgae is limited by the high cost of CO(2) capture and transportation, as well as significant CO(2) loss during algae culture. Moreover, algae grow poorly at night, but CO(2) cannot be temporarily stored until sunrise. To address these challenges, we discuss a process where CO(2) is captured as bicarbonate and used as feedstock for algae culture, and the carbonate regenerated by the culture process is used as an absorbent to capture more CO(2). This process would significantly reduce carbon capture costs because it does not require additional energy for carbonate regeneration. Furthermore, not only would transport of the aqueous bicarbonate solution cost less than for that of compressed CO(2), but using bicarbonate would also provide a superior alternative for CO(2) delivery to an algae culture system.

  20. Relationships between food producers and retail chains seen as shared meanings

    Skytte, Hans

    This paper presents a new theory on relationships between producers and retail chains. This theory is a result of a project which investigated the cooperation between Danish abattoirs and food processors, and retail chains in four countries. The new theory's main point is that relationships between...... producers and retail chains are developed through construction of inter-organizational shared meanings - shared meanings which are developed through recursive patterns. To position the new theory, the paper first gives a short presentation of traditional approaches to relationship marketing followed...... by a brief presentation of the paradigm used for the development of the new theory. After that you will find a section dealing with the conceptual framework. This section is followed by a discussion of the main results. The paper ends with a short discussion of implications for management....

  1. Modeling and computation of mean field equilibria in producers' game with emission permits trading

    Zhang, Shuhua; Wang, Xinyu; Shanain, Aleksandr


    In this paper, we present a mean field game to model the production behaviors of a very large number of producers, whose carbon emissions are regulated by government. Especially, an emission permits trading scheme is considered in our model, in which each enterprise can trade its own permits flexibly. By means of the mean field equilibrium, we obtain a Hamilton-Jacobi-Bellman (HJB) equation coupled with a Kolmogorov equation, which are satisfied by the adjoint state and the density of producers (agents), respectively. Then, we propose a so-called fitted finite volume method to solve the HJB equation and the Kolmogorov equation. The efficiency and the usefulness of this method are illustrated by the numerical experiments. Under different conditions, the equilibrium states as well as the effects of the emission permits price are examined, which demonstrates that the emission permits trading scheme influences the producers' behaviors, that is, more populations would like to choose a lower rather than a higher emission level when the emission permits are expensive.

  2. Promoting and recovering health: meanings produced in community groups within the family health program context

    Celiane Camargo-Borges


    Full Text Available Public Healthcare in Brazil has tended to reflect major changes in the healthcare model. New tendencies point to the importance of actions being built up from their context and focused on specific communities. The objective of this study is to describe the meanings of health / illness as produced by community groups within the context of a Family Healthcare Program. Five groups had their single-session discussions taped and recorded, under the coordination of the first author. This material was transcribed and, coupled with field notes, formed the database for this study. The analysis described the meanings of the ideas on which new healthcare proposals are being based, providing visibility for the multiplicity of meanings and denaturalizing fixed lines of discourse on healthcare / illness. The final thoughts, developed from the point of view of social constructionism, indicate that healthcare practices based on the process of constant conversation and negotiation between all the social actors involved is a fertile ground.

  3. Intelligence and culture: how culture shapes what intelligence means, and the implications for a science of well-being.

    Sternberg, Robert J; Grigorenko, Elena L


    This paper discusses the relationship between culture and intelligence. The main message of the paper is that intelligence cannot fully or even meaningfully be understood outside its cultural context. Behaviour that is considered intelligent in one culture may be considered unintelligent in another culture, and vice versa. Moreover, people in different cultures have different implicit (folk) theories of intelligence, so may not even mean the same thing by the word. The relationships between different aspects of intelligence can vary across cultures, with correlations that are positive in one setting proving to be negative in another. The paper opens with a general discussion of issues regarding the relationship between the two concepts. It then describes the theory of successful intelligence, which motivates our work on the interface between culture and intelligence. Finally, the article draws some conclusions.

  4. The Impact of Cultural Context on Metaphorical Meaning in English and Chinese

    WEI Meng-fen


    Metaphor is a phenomenon of language use which is the most active and most able to reflect the cultural factor. Cul⁃tural context has a restrictive effect on the metaphorical meaning. Therefore, the understanding and use of metaphor must not be divorced from the underlying cultural context. This paper analyzes similarities and dissimilarities of metaphorical meaning and im⁃ages in English and Chinese from the perspective of cultural context. The research of metaphor of cultural context would enable us to better understand metaphor and have a successful communication.

  5. Distribution of binder in granules produced by means of twin screw granulation.

    Fonteyne, Margot; Fussell, Andrew Luke; Vercruysse, Jurgen; Vervaet, Chris; Remon, Jean Paul; Strachan, Clare; Rades, Thomas; De Beer, Thomas


    According to the quality by design principle processes may not remain black-boxes and full process understanding is required. The granule size distribution of granules produced via twin screw granulation is often found to be bimodal. The aim of this study was to gain a better understanding of binder distribution within granules produced via twin screw granulation in order to investigate if an inhomogeneous spread of binder is causing this bimodal size distribution. Theophylline-lactose-polyvinylpyrrolidone K30 (PVP) (30-67.5-2.5%, w/w) was used as a model formulation. The intra-granular distribution of PVP was evaluated by means of hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy. For the evaluated formulation, no PVP rich zones were detected when applying a lateral spatial resolution of 0.5 μm, indicating that PVP is homogenously distributed within the granules.

  6. Biomass recycle as a means to improve the energy efficiency of CELSS algal culture systems

    Radmer, R.; Cox, J.; Lieberman, D.; Behrens, P.; Arnett, K.


    Algal cultures can be very rapid and efficient means to generate biomass and regenerate the atmosphere for closed environmental life support systems. However, as in the case of most higher plants, a significant fraction of the biomass produced by most algae cannot be directly converted to a useful food product by standard food technology procedures. This waste biomass will serve as an energy drain on the overall system unless it can be efficiently recycled without a significant loss of its energy content. Experiments are reported in which cultures of the alga Scenedesmus obliquus were grown in the light and at the expense of an added carbon source, which either replaced or supplemented the actinic light. As part of these experiments, hydrolyzed waste biomass from these same algae were tested to determine whether the algae themselves could be made part of the biological recycling process. Results indicate that hydrolyzed algal (and plant) biomass can serve as carbon and energy sources for the growth of these algae, suggesting that the efficiency of the closed system could be significantly improved using this recycling process.

  7. Meme Engineers: Children as Producers of Musical Culture

    Barrett, Margaret


    This paper examines the ways in which young children construct and negotiate musical meaning as song-makers in a post-modern consumerist musical world. A critical analysis of the presentation of music in two popular Australian children's television programmes (Play School and Hi-5) and the ways in which such media impact upon children's…

  8. Meme Engineers: Children as Producers of Musical Culture

    Barrett, Margaret


    This paper examines the ways in which young children construct and negotiate musical meaning as song-makers in a post-modern consumerist musical world. A critical analysis of the presentation of music in two popular Australian children's television programmes (Play School and Hi-5) and the ways in which such media impact upon children's…

  9. The Meaning of English Words across Cultures, with a Focus on Cameroon and Hong Kong

    Bobda, Augustin Simo


    A word, even when considered monosemic, generally has a cluster of meanings, depending on the mental representation of the referent by the speaker/writer or listener/reader. The variation is even more noticeable across cultures. This paper investigates the different ways in which cultural knowledge helps in the interpretation of English lexical…

  10. Overcoming the Discontinuity of Patriotic Education and Moral Development by Means of Regional Culture

    Tikhonova, Anna U.


    The paper analyzes studies on continuing education, explains the concept of "continuity of patriotic education and moral development by means of regional culture", determines the uniqueness of regional culture as the core of patriotic education and moral development, and characterizes the model of continuity of patriotic education and…

  11. What images are teens producing? - Visual culture, education and adolescences

    Anderson Ferrari


    Full Text Available The article is the result of a survey of Undergraduate Research held at four schools in the city of Juiz de Fora with male and female students of the second segment of Elementary Education, with the aim of investigating and questioning the relationships that are established between young people and schools in their links to the pictures and learn. Rather than focus on the relationship between Visual Culture, Education and subjectivities, the text invests in new goals and work proposals that dialogue with my performances in the research groups and the study of gender and sexuality UFJF. In this sense, I am also interested in the relationships of these adolescents with gender relations, sexualities and images. As the pictures looks to educate our genders and sexualities?

  12. High-Level Culturability of Epiphytic Bacteria and Frequency of Biosurfactant Producers on Leaves

    Burch, Adrien Y.; Do, Paulina T.; Sbodio, Adrian; Suslow, Trevor V.


    ABSTRACT To better characterize the bacterial community members capable of biosurfactant production on leaves, we distinguished culturable biosurfactant-producing bacteria from nonproducers and used community sequencing to compare the composition of these distinct cultured populations with that from DNA directly recovered from leaves. Communities on spinach, romaine, and head lettuce leaves were compared with communities from adjacent samples of soil and irrigation source water. Soil communities were poorly described by culturing, with recovery of cultured representatives from only 21% of the prevalent operational taxonomic units (OTUs) (>0.2% reads) identified. The dominant biosurfactant producers cultured from soil included bacilli and pseudomonads. In contrast, the cultured communities from leaves are highly representative of the culture-independent communities, with over 85% of the prevalent OTUs recovered. The dominant taxa of surfactant producers from leaves were pseudomonads as well as members of the infrequently studied genus Chryseobacterium. The proportions of bacteria cultured from head lettuce and romaine leaves that produce biosurfactants were directly correlated with the culture-independent proportion of pseudomonads in a given sample, whereas spinach harbored a wider diversity of biosurfactant producers. A subset of the culturable bacteria in irrigation water also became enriched on romaine leaves that were irrigated overhead. Although our study was designed to identify surfactant producers on plants, we also provide evidence that most bacteria in some habitats, such as agronomic plant surfaces, are culturable, and these communities can be readily investigated and described by more classical culturing methods. IMPORTANCE The importance of biosurfactant production to the bacteria that live on waxy leaf surfaces as well as their ability to be accurately assessed using culture-based methodologies was determined by interrogating epiphytic populations by

  13. Cloned calves produced by nuclear transfer from cultured cumulus cells

    AN; Xiaorong(安晓荣); GOU; Kemian(苟克勉); ZHU; Shien(朱士恩); GUAN; Hong(关宏); HOU; Jian(侯健); LIN; Aixing(林爱星); ZENG; Shenming(曾申明); TIAN; Jianhui(田见辉); CHEN; Yongfu(陈永福)


    Short-term cultured cumulus cell lines (1-5BCC) derived from 5 individual cows were used in nuclear transfer (NT) and 1188 enucleated bovine oocytes matured in vitro were used as nuclear recipients. A total of 931 (78.4%) cloned embryos were reconstructed, of which 763 (82%) cleaved, 627 (67.3%) developed to 8-cell stage, and 275 (29.5%) reached blastocyst stage. The average cell number of blastocysts was 124±24.5 (n=20). In this study, the effects of donor cell sources, serum starvation of donor cells, time interval from fusion to activation (IFA) were also tested on cloning efficiency. These results showed that blastocyst rates of embryos reconstructed from 5 different individuals cells were significantly different among them (14.1%, 45.2%, 27.3%, 34.3%, vs 1.5%, P0.05); and that blastocyst rate (20.3%) of the group with fusion/activation interval of 2-3 h, was significantly lower than that of the 3-6 h groups (31.0%), while not significantly different among 3-4 h (P < 0.05), 4-5 h, and 5-6 h groups (P ≥ 0.05). Sixty-three thawed NT blastocysts were transferred to 31 recipient cows, of which 4 pregnancies were established and two cloned calves were given birth. These results indicate that serum starvation of cumulus cells is not a key factor for successful bovine cloning, while IFA treatment and sources of donor cells have effects on cloning efficiency.

  14. Preconceptual Feasibility Study to Evaluate Alternative Means to Produce Plutonium-238

    John D. Bess; Matthew S. Everson


    There is currently no large-scale production of 238Pu in the United States. Feasibility studies were performed at the Idaho National Laboratory to assess the capability of developing alternative 238Pu production strategies. Initial investigations indicate potential capability to provision radioisotope-powered systems for future space exploration endeavors. For the short term production of 238Pu, sealed canisters of dilute 237Np solution in nitric acid could be irradiated in the Advanced Test Reactor (ATR). Targets in the large and medium “I” positions of the ATR were irradiated over a simulated period of 306 days and analyzed using MCNP5 and ORIGEN2.2. Approximately 0.5 kg of 238Pu could be produced annually in the ATR with purity greater than 92%. Optimization of the irradiation cycles could further increase the purity to greater than 98%. Whereas the typical purity of space batteries is between 80 to 85%, the higher purity 238Pu produced in the ATR could be blended with existing lower-purity inventory to produce useable material. Development of irradiation methods in the ATR provides the fastest alterative to restart United States 238Pu production. The analysis of 238Pu production in the ATR provides the technical basis for production using TRIGA® (Training, Research, Isotopes, General Atomics) nuclear reactors. Preliminary analyses envisage a production rate of approximately 0.7 kg annually using a single dedicated 5-MW TRIGA reactor with continuous flow loops to achieve high purity product. Two TRIGA reactors represent a robust means of providing at over 1 kg/yr of 238Pu annually using dilute solution targets of 237Np in nitric acid. Further collaboration and optimization of reactor design, radiochemical methods, and systems analyses would further increase annual 238Pu throughput, while reducing the currently evaluated reactor requirements.

  15. Meaning and Identities: A Visual Performative Pedagogy for Socio-Cultural Learning

    Grushka, Kathryn


    In this article I present personalised socio-cultural inquiry in visual art education as a critical and expressive material praxis. The model of "Visual Performative Pedagogy and Communicative Proficiency for the Visual Art Classroom" is presented as a legitimate means of manipulating visual codes, communicating meaning and mediating…

  16. Gene probes to detect cross-culture contamination in hormone producing cell lines

    Matsuba, I; Lernmark, A; Madsen, Ole Dragsbæk


    Cross-culture contamination of cell lines propagated in continuous culture is a frequent event and particularly difficult to resolve in cells expressing similar phenotypes. We demonstrate that DNA-DNA hybridization to blotted endonuclease-digested cell DNA effectively detects cross-culture...... the effective use of gene probes to control the origin of cell cultures....... contamination to monitor inter-species as well as intra-species cross contamination. An insulin-producing cell-line, Clone-16, originally cloned from a human fetal endocrine pancreatic cell line did not produce human c-peptide as anticipated. DNA from these cells showed no hybridization to the human ALU...

  17. Cybersemiotics: A suggestion for a transdisciplinary framework for description of observing, anticipatory and meaning producing systems

    Brier, Soren


    The ability of systems to be anticipatory seems to be intricate connected with the ability to observe and to cognate by reducing complexity through signification. The semantic capacity of living systems, the cognitive ability to assign meaning to differences perturbating the system's self-organization, seems to be the prerequisite for the phenomenon of communication, language and consciousness. In cybernetics Bateson developed the idea that information is a difference that makes a difference and second order cybernetics developed the concept of organisms as self-organized and self-produced systems (autopoietic) as the prerequisite of life and cognition. The cognitive ability seems to be qualitative different from what so far is computable on any known machine although parts of different aspects of the process can be partly simulated in AI, neutral network and AL. In semiotics the fundamental process of cognition and communication is called semiosis or signification and C. S. Peirce created a special triadic, objective idealistic, pragmatic and evolutionary philosophy to be able to give a fruitful description of the process and its relation to logic and the concept of natural law. Both second order cybernetics and semiotics sees information and meaning as something produced by individual organisms through structural couplings to the environments or other individuals through historical drift and further developed in social communication. Luhmann points out that social communication also only functions through structural couplings which he calls generalized media such as science, art, power, love and money. Peirce talks of the semiotic net as a triadic view of meanings developing through history and in animals through evolution. In accordance with this Wittgenstein points out that signification is created in language games developed in specific life forms. Life forms are the things we do in society such as seducing, commanding and explaining. As animals do not have

  18. Cultural aspects of bipolar disorder: Interpersonal meaning for clients & psychiatric nurses.

    Warren, Barbara Jones


    Bipolar disorder is a complicated mental illness to diagnose and treat. The symptoms of the disorder cause a multitude of fluctuations in mood and behavior, affecting the way individuals function and interact with others on a daily basis. Individuals diagnosed with bipolar disorder experience symptoms within a framework that is grounded in their cultural beliefs, values, and norms. Culture is a complex and personal biopsychosocial phenomenon that provides meaning within life for an individual, a group, or a community. It is essential that psychiatric-mental health (PMH) nurses understand the role of culture and integrate this knowledge into the biopsychosocial care of clients. The development and maintenance of the interpersonal therapeutic relationship between PMH nurses and their clients requires the use of a cultural framework, which refers to the connection of culture and cultural competence. The purposes of this article are to define culture and the process of cultural competence, provide a brief overview of bipolar disorder, propose the use of a cultural framework for bipolar disorder, and discuss the implications for PMH nurses who care for culturally and ethnically diverse clients.

  19. Bench to batch: advances in plant cell culture for producing useful products.

    Weathers, Pamela J; Towler, Melissa J; Xu, Jianfeng


    Despite significant efforts over nearly 30 years, only a few products produced by in vitro plant cultures have been commercialized. Some new advances in culture methods and metabolic biochemistry have improved the useful potential of plant cell cultures. This review will provide references to recent relevant reviews along with a critical analysis of the latest improvements in plant cell culture, co-cultures, and disposable reactors for production of small secondary product molecules, transgenic proteins, and other products. Some case studies for specific products or production systems are used to illustrate principles.

  20. The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination

    Mu, Yang; Yang, Hou-Yun; Wang, Ya-Zhou; He, Chuan-Shu; Zhao, Quan-Bao; Wang, Yi; Yu, Han-Qing


    Fermentative hydrogen production from wastes has many advantages compared to various chemical methods. Methodology for characterizing the hydrogen-producing activity of anaerobic mixed cultures is essential for monitoring reactor operation in fermentative hydrogen production, however there is lack of such kind of standardized methodologies. In the present study, a new index, i.e., the maximum specific hydrogen-producing activity (SHAm) of anaerobic mixed cultures, was proposed, and consequently a reliable and simple method, named SHAm test, was developed to determine it. Furthermore, the influences of various parameters on the SHAm value determination of anaerobic mixed cultures were evaluated. Additionally, this SHAm assay was tested for different types of substrates and bacterial inocula. Our results demonstrate that this novel SHAm assay was a rapid, accurate and simple methodology for determining the hydrogen-producing activity of anaerobic mixed cultures. Thus, application of this approach is beneficial to establishing a stable anaerobic hydrogen-producing system.

  1. In vitro cell cultures obtained from different explants of Corylus avellana produce Taxol and taxanes

    Cavalli Francesca


    Full Text Available Abstract Background Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy the commercial requirements. The aim of the present work was to produce suspension cell cultures from plants not belonging to Taxus genus and to verify whether they produced Taxol and taxanes. For this purpose different explants of hazel (Corylus avellana species were used to optimize the protocol for inducing in vitro callus, an undifferentiated tissue from which suspension cell cultures were established. Results Calli were successfully induced from stems, leaves and seeds grown in various hormone concentrations and combinations. The most suitable callus to establish suspension cell cultures was obtained from seeds. Media recovered from suspension cell cultures contained taxanes, and showed antiproliferative activity on human tumour cells. Taxol, 10-deacetyltaxol and 10-deacetylbaccatin III were the main taxanes identified. The level of Taxol recovered from the media of hazel cultures was similar to that found in yew cultures. Moreover, the production of taxanes in hazel cell cultures increased when elicitors were used. Conclusion Here we show that hazel cell cultures produce Taxol and taxanes under controlled conditions. This result suggests that hazel possesses the enzymes for Taxol production, which until now was considered to be a pathway particular to Taxus genus. The main benefit of producing taxanes through hazel cell cultures is that hazel is widely available, grows at a much faster rate in vivo, and is easier to cultivate in vitro than yew. In addition, the production of callus directly from hazel seeds shortens the culture time and minimizes the probability of contamination. Therefore, hazel could become a

  2. Cross-Cultural Counseling and Cross-Cultural Meanings: An Exploration of Morita Psychotherapy.

    Aldous, Jane L.


    Describes theoretical framework and techniques of Morita psychotherapy. Western research indicates that Asian American clients prefer active-directive, logical, rational, and structured approaches. Suggests that ethnocentric counseling approaches may be imposed upon clients of Asian origin because meanings attached to terms describing counseling…

  3. Children's activities and their meanings for parents: a mixed-methods study in six Western cultures.

    Harkness, Sara; Zylicz, Piotr Olaf; Super, Charles M; Welles-Nyström, Barbara; Bermúdez, Moisés Ríos; Bonichini, Sabrina; Moscardino, Ughetta; Mavridis, Caroline Johnston


    Theoretical perspectives and research in sociology, anthropology, sociolinguistics, and cultural psychology converge in recognizing the significance of children's time spent in various activities, especially in the family context. Knowing how children's time is deployed, however, only gives us a partial answer to how children acquire competence; the other part must take into account the culturally constructed meanings of activities, from the perspective of those who organize and direct children's daily lives. In this article, we report on a study of children's routine daily activities and on the meanings that parents attribute to them in six Western middle-class cultural communities located in Italy, The Netherlands, Poland, Spain, Sweden, and the United States (N = 183). Using week-long time diaries kept by parents, we first demonstrate similarities as well as significant differences in children's daily routines across the cultural samples. We then present brief vignettes--"a day in the life" --of children from each sample. Parent interviews were coded for themes in the meanings attributed to various activities. Excerpts from parent interviews, focusing on four major activities (meals, family time, play, school- or developmentally related activities), are presented to illustrate how cultural meanings and themes are woven into parents' organization and understanding of their children's daily lives. The results of this mixed-method approach provide a more reliable and nuanced picture of children's and families' daily lives than could be derived from either method alone.

  4. Symbolic Meanings of High and Low Impact Daily Consumption Practices in Different Cultures

    Ger, Güliz; Wilhite, Harold; Halkier, Bente


    Daily consumption practices are not only practical doings. They also express symbolic meanings; social signals by which we shape our identity, confirm or challenge cultural conventions and construct images of the good life. The symbolic meanings are collectively shared and negotiated....... They influence our choices and practices whether it is by motivating or impeding them. In this study we will look at the symbolic meanings of high and low environmental impact consumption practices. In order to create desirable social markers for environmentally-friendly practices and/or to change high impact...... practices, we need to understand emergent practices and their existing cultural meanings. Thus we have chosen three fields of daily consumption practices - food consumption, transport and hygiene - and sorted out the relatively environmentally friendly (low impact) and the relatively environmentally...

  5. How Do Cultural Producers Make Creative Decisions? Lessons from the Catwalk

    Godart, Frederic C. Mears, Ashley


    Faced with high uncertainty, how do producers in the cultural economy make creative decisions? We present a case study of the fashion modeling industry. Using participant observation, interviews and network analysis of the Spring/Summer 2007 Fashion Week collections, we explain how producers select models for fashion shows. While fashion producers…

  6. Celebrating the "Intellectual Heart": Emergent Meanings from a Post-Earthquake Organizational Communication Culture Perspective.

    McNamara, Catherine V.; And Others

    The Organizational Communication Culture (OCC) method, designed by Charles A. Bantz (1993) was utilized as the primary method for analyzing messages coming from the administration of California State University, Northridge, after the earthquake. Bantz feels that communication interactions in an organization bring meanings and expectations to that…

  7. Cultural Interpretations of the Visual Meaning of Icons and Images Used in North American Web Design

    Knight, Eliot; Gunawardena, Charlotte N.; Aydin, Cengiz Hakan


    This study examines cross-cultural interpretations of icons and images drawn from US academic websites. Participants from Morocco, Sri Lanka, Turkey, and the USA responded to an online questionnaire containing 18 icons and images representing online functions and information types common on US academic websites. Participants supplied meanings for…

  8. Rethinking the Adventure Education Experience: An Inquiry of Meanings, Culture and Educational Virtue

    Ingman, Benjamin Charles


    This study is an investigation of the adventure education (AE) experience with particular attention to what happens during the AE experience, the meanings participants ascribe to the experience, how personal backgrounds and institutional cultures coalesce in AE, and the significance of the AE experience for schooling. These topics are explored…

  9. Viewing as a Cultural Tool in the Construction of Meaning with Expository Texts for Young Bilinguals

    Moses, Lindsey


    This article provides a deeper understanding of how young bilinguals constructed meaning with expository texts in an inquiry-based setting over an academic year. An examination of classroom transcripts and analysis revealed how "Viewing" became the most frequently used literacy practice and a cultural tool in the construction of meaning…

  10. The English Language as a Means of Expressing African Culture: A Conflicting Issue.

    Arezzo, Ersilia La Pergola


    In the context of the works of African writers Chinua Achebe and Ngugi Wa Thiong'o, the complexity and controversy surrounding the adoption of English in African literary texts is discussed. The appreciation of English as a means of international communication is reconciled with the desire to preserve the languages and cultural identities of the…

  11. The Ububele Baby Mat intervention: facilitating meaning in a multi-cultural context.

    Nortje, Michelle


    This paper expands on the continuing understanding of the Ububele Baby Mat Project - a community-based parent-infant mental health intervention now offered at six primary healthcare clinics in Alexandra, Johannesburg. This paper describes the influence of cultural diversity and the complex layers of meaning-making involved in the Baby Mat intervention. Meaning-making is a collaborative process between the caregiver-infant dyad's cultural beliefs and experiences, the Baby Mat couple's relationship, knowledge and skills, and the additional minds of the multicultural supervision group. This paper aims to clarify these three layers of co-constructed meaning-making involved in the Baby Mat intervention. Three central themes are thus presented to discuss the impact of multi-cultural, multi-racial and multi-lingual therapeutic dyads on the process of the Baby Mat intervention. These core themes are the verbal and non-verbal meanings of presenting problems brought by caregiver-infant dyads; the value and obstacles involved within a cross-cultural baby mat couple; and the significance of a reflective group supervision space. Case extracts are used to illustrate these processes of multiple minds at play during a Baby Mat session.

  12. Sensorimotor Control of Sound-Producing Gestures, Musical Gestures - Sound, Movement, and Meaning

    Gibet, Sylvie


    In this chapter, we focus on sensorimotor models of sound-producing gestures. These models are studied from two different viewpoints, namely theories for motor control, and computer synthesis of avatars that produce human gesture. The theories aim to understand gesture on the basis of the underlying biomechanics, whereas the computer synthesis aims to understand entire gestures on the basis ofsensorimotor control models The emphasis of this chapter is on hand-arm gestures, from simple control...

  13. High-Level Culturability of Epiphytic Bacteria and Frequency of Biosurfactant Producers on Leaves

    Burch, AY; Do, PT; Sbodio, A; Suslow, TV; Lindow, SE


    To better characterize the bacterial community members capable of biosurfactant production on leaves, we distinguished culturable biosurfactant-producing bacteria from nonproducers and used community sequencing to compare the composition of these distinct cultured populations with that from DNA directly recovered from leaves. Communities on spinach, romaine, and head lettuce leaves were compared with communities from adjacent samples of soil and irrigation source water. Soil communities were ...

  14. Coevolution of adaptive technology, maladaptive culture and population size in a producer-scrounger game.

    Lehmann, Laurent; Feldman, Marcus W


    Technology (i.e. tools, methods of cultivation and domestication, systems of construction and appropriation, machines) has increased the vital rates of humans, and is one of the defining features of the transition from Malthusian ecological stagnation to a potentially perpetual rising population growth. Maladaptations, on the other hand, encompass behaviours, customs and practices that decrease the vital rates of individuals. Technology and maladaptations are part of the total stock of culture carried by the individuals in a population. Here, we develop a quantitative model for the coevolution of cumulative adaptive technology and maladaptive culture in a 'producer-scrounger' game, which can also usefully be interpreted as an 'individual-social' learner interaction. Producers (individual learners) are assumed to invent new adaptations and maladaptations by trial-and-error learning, insight or deduction, and they pay the cost of innovation. Scroungers (social learners) are assumed to copy or imitate (cultural transmission) both the adaptations and maladaptations generated by producers. We show that the coevolutionary dynamics of producers and scroungers in the presence of cultural transmission can have a variety of effects on population carrying capacity. From stable polymorphism, where scroungers bring an advantage to the population (increase in carrying capacity), to periodic cycling, where scroungers decrease carrying capacity, we find that selection-driven cultural innovation and transmission may send a population on the path of indefinite growth or to extinction.

  15. Material Culture and Cultural Meanings: Current Studies of Archaeological Ceramics and in Ce­ramic Ethnoarchaeology

    Charles C. Kolb


    Full Text Available Pottery and People: A Dynamic Interaction. James M. Skibo and Gary M. Feinman (editors. Founda­tions of Archaeological Inquiry. Salt Lake City. University of Utah Press. 1999. 260 pp. 91 figures. 30 tables. ISBN 0-87480-576-7. $55.00 (cloth; 0-87489-577-5. $25.00 (paperback. Material Meanings: Critical Approaches to the Interpretation of Material Culture. Elizabeth S. Chilton (editor. Foundations of Archaeological Inquiry. Salt Lake City: University of Utah Press. 1999.179pp. 35 figures. 11 tables. ISBN 0-87480-632-1. $55.00 (cloth; 087480-633-X. $35.00 (paperback. The description and interpretation of material culture may be regarded as the essence of archaeology, a discipline that seeks to recover, describe, document, and interpret past human culture. More recently, understanding that actions occur in a material world that is constituted symbolically, archaeological explanations are often framed in sociocultural meanings, the analysis of agencies, practices and behaviors. I shall subsequently return to this issue. Because of their longevity in the archaeological record, lithic and ceramic artifacts are crucial to the endeavor to interpret human culture. Objects fashioned from clay and subjected to intentional artificial sources of heat made their initial appearance in the archaeological record more than 26.000 years ago. Ceramic objects have been created in a seemingly endless variety of shapes and forms, varying from fertility figurines. to cooking and food storage vessels. lamps, smoking pipes, medicinal pastilles, tokens, beehives, and coffins to modern whitewares and pyroceramics.

  16. A strict anaerobic extreme thermophilic hydrogen-producing culture enriched from digested household waste

    Karakashev, Dimitar Borisov; Kotay, Shireen Meher; Trably, Eric;


    The aim of this study was to enrich, characterize and identify strict anaerobic extreme thermophilic hydrogen (H-2) producers from digested household solid wastes. A strict anaerobic extreme thermophilic H-2 producing bacterial culture was enriched from a lab-scale digester treating household was...... from digested household wastes. This study provided a culture with a potential to be applied in reactor systems for extreme thermophilic H-2 production from complex organic wastes.......The aim of this study was to enrich, characterize and identify strict anaerobic extreme thermophilic hydrogen (H-2) producers from digested household solid wastes. A strict anaerobic extreme thermophilic H-2 producing bacterial culture was enriched from a lab-scale digester treating household...... sources. Growth on glucose produced acetate, H-2 and carbon dioxide. Maximal H-2 production rate on glucose was 1.1 mmol l(-1) h(-1) with a maximum H-2 yield of 1.9 mole H-2 per mole glucose. 16S ribosomal DNA clone library analyses showed that the culture members were phylogenetically affiliated...

  17. Finding meaning in first episode psychosis: experience, agency, and the cultural repertoire.

    Larsen, John Aggergaard


    The article examines individuals' attempts to generate meaning following their experiences with psychosis. The inquiry is based on a person-centered ethnographic study of a Danish mental health community program for early intervention in schizophrenia and involves longitudinal interviews with 15 of its participants. The article takes an existential anthropological perspective emphasizing agency and cultural phenomenology to investigate how individuals draw on resources from the cultural repertoire to make sense of personally disturbing experiences during their psychosis. It is suggested that the concept of "system of explanation" has advantages over, for example, "illness narrative" and "explanatory model" when demonstrating how some individuals engage in the creative analytic and theory-building work of bricolage, selecting, adding, and combining various systems of explanation. Delusions are equally derived from the cultural repertoire but are constructed as dogmatic explanations that are idiosyncratic to the individual who holds them.

  18. The Cultural Voice of Immigrant Latina Women and the Meaning of Femininity

    Ellen Greer


    Full Text Available This phenomenological study investigates the cultural meaning of femininity to immigrant Latina women and its significance in the consideration of decisions related to maintaining breast health. Theories of culture and health promotion support the concern for women’s need for access to health care in relation to breast health and the cultural barriers that interface between the connections of femininity, body image, and mental health. For Hispanic women, breast cancer is the most commonly diagnosed cancer generating distress for the individual, partner, and family. The importance of best practices in health promotion and risk reduction strategies in the early screening of women is emphasized in the literature. However, the voice of immigrant Latina women in describing their perspective of femininity and breast health and how it may affect choice and decision making related to breast self-care practices has not been studied. Five themes emerged from the data analysis conducted with Giorgi’s phenomenological method leading to an unfolded description of femininity: power of feminine identity through motherhood, hardiness is sustainability to overcoming adversity, connection to self and others, satisfaction meeting cultural gender-role expectations, and contemplative prevention to maintain breast integrity. The findings of this study will contribute to the increasing body of evidence-based practice related to understanding the impact of culture related to breast health. While the concept of femininity can be challenged not only by a diagnosis of breast cancer but also by other diseases and life conditions, understanding the cultural meaning of femininity to Latina women is pivotal to health care professionals as they partner with Latina women and community support groups to develop empowerment strategies and programs that promote choice and decision making involving breast health.

  19. ‘‘We are like lemmings’’: making sense of the cultural meaning(s) of suicide among the indigenous Sami in Sweden

    Stoor, Jon Petter A; Kaiser, Niclas; Jacobsson, Lars; Salander Renberg, Ellinor; Silviken, Anne


    Background. Suicide is a widespread problem among indigenous people residing in the circumpolar Arctic. Though the situation among the indigenous Sami in northern Scandinavia is better than among some other indigenous people, suicide is still regarded as a major public health issue. To adapt prevention strategies that are culturally attuned one must understand how suicide is understood within context. That is, the cultural meaning(s) of suicide.Objective. To explore and make sense of the cult...

  20. Relationships between food producers and retail chains seen as shared meanings

    Skytte, Hans

    This paper presents a new theory on relationships between producers and retail chains. This theory is a result of a project which investigated the cooperation between Danish abattoirs and food processors, and retail chains in four countries. The new theory's main point is that relationships betwe...

  1. Production of mycotoxins by galactose oxidase producing Fusarium using different culture

    Pereira Angela Maria


    Full Text Available The original isolate of the galactose oxidase producing fungus Dactylium dendroides, and other five galactose oxidase producing Fusarium isolates were cultivated in different media and conditions, in order to evaluate the production of 11 mycotoxins, which are characteristic of the genus Fusarium: moniliformin, fusaric acid, deoxynivalenol, fusarenone-X, nivalenol, 3-acetyldeoxynivalenol, neosolaniol, zearalenol, zearalenone, acetyl T-2, and iso T-2. The toxicity of the culture extracts to Artemia salina larvae was tested.

  2. Distribution of binder in granules produced by means of twin screw granulation

    Fonteyne, Margot; Fussell, Andrew Luke; Vercruysse, Jurgen


    -granular distribution of PVP was evaluated by means of hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy. For the evaluated formulation, no PVP rich zones were detected when applying a lateral spatial resolution of 0.5 μm, indicating that PVP is homogenously distributed within the granules....

  3. Hardfacing of aluminium alloys by means of metal matrix composites produced by laser surface alloying

    Pityana, SL


    Full Text Available Metal matrix composite layers were formed on an aluminium substrate by means of laser surface alloying method. Aluminium 1200 was used as a host material and TiC particles were used as the reinforcement. The microstructure of the modified layer...

  4. Resveratrol Produces Neurotrophic Effects on Cultured Dopaminergic Neurons through Prompting Astroglial BDNF and GDNF Release

    Feng Zhang


    Full Text Available Increasing evidence indicated astroglia-derived neurotrophic factors generation might hold a promising therapy for Parkinson’s disease (PD. Resveratrol, naturally present in red wine and grapes with potential benefit for health, is well known to possess a number of pharmacological activities. Besides the antineuroinflammatory properties, we hypothesized the neuroprotective potency of resveratrol is partially due to its additional neurotrophic effects. Here, primary rat midbrain neuron-glia cultures were applied to investigate the neurotrophic effects mediated by resveratrol on dopamine (DA neurons and further explore the role of neurotrophic factors in its actions. Results showed resveratrol produced neurotrophic effects on cultured DA neurons. Additionally, astroglia-derived neurotrophic factors release was responsible for resveratrol-mediated neurotrophic properties as evidenced by the following observations: (1 resveratrol failed to exert neurotrophic effects on DA neurons in the cultures without astroglia; (2 the astroglia-conditioned medium prepared from astroglia-enriched cultures treated with resveratrol produced neurotrophic effects in neuron-enriched cultures; (3 resveratrol increased neurotrophic factors release in the concentration- and time-dependent manners; (4 resveratrol-mediated neurotrophic effects were suppressed by blocking the action of the neurotrophic factors. Together, resveratrol could produce neurotrophic effects on DA neurons through prompting neurotrophic factors release, and these effects might open new alternative avenues for neurotrophic factor-based therapy targeting PD.

  5. Trichoderma L-Lysine-α-Oxidase Producer Strain Culture Fluid Inhibits Impatiens Necrotic Spot Virus.

    Smirnova, I P; Shneider, Yu A; Karimova, E V


    A method for PCR diagnosis of impatiens necrotic spot virus is developed. Concentrated culture fluid with active L-lysine-α-oxidase (0.54 U/ml) from Trichoderma harzianum Rifai fungus producer strain F-180 inhibits vitally hazardous impatiens necrotic spot phytovirus.

  6. Lipolytic Changes in Fermented Sausages Produced with Turkey Meat: Effects of Starter Culture and Heat Treatment

    Karsloğlu, Betül; Çiçek, Ümran Ensoy; KOLSARICI, Nuray; Candoğan, Kezban


    In this study, the effects of two different commercial starter culture mixes and processing methodologies (traditional and heat process) on the lipolytic changes of fermented sausages manufactured with turkey meat were evaluated during processing stages and storage. Free fatty acid (FFA) value increased with fermentation and during storage over 120 d in all fermented sausage groups produced with both processing methodologies (p

  7. Lipolytic Changes in Fermented Sausages Produced with Turkey Meat: Effects of Starter Culture and Heat Treatment

    Karsloğlu, Betül; Çiçek, Ümran Ensoy; KOLSARICI, Nuray; CANDOĞAN, Kezban


    In this study, the effects of two different commercial starter culture mixes and processing methodologies (traditional and heat process) on the lipolytic changes of fermented sausages manufactured with turkey meat were evaluated during processing stages and storage. Free fatty acid (FFA) value increased with fermentation and during storage over 120 d in all fermented sausage groups produced with both processing methodologies (p

  8. [Culture-filtrate producing condition and biological activity of Fusarium solani].

    Ding, Wenjiao; Li, Jinhua; Chai, Zhaoxiang


    To study the culture-filtrate producing condition of Fusarium Solani isolated from Astragalus root and explore the mechanism Astragalus root rot disease caused by, in order to find theoretical support for screening resistant germ plasma via mycotoxin. The method of germinating seeds in petri dish with filter paper and inhibition method for embryo growth were used to study the biological activity and the specialty of cultural filtrate of 10 F. solani isolates. The toxin produced by F. solani had strong inhibition effect in the different nutrient media, at different temperatures and under different light conditions. With extension of culturing time, embryo inhibition rate went up gradually with the strongest inhibition at the 12th day and the inhibition ratio between 92.0% -52.0%. The toxin produced at 5 degrees C to 35 degrees C inhibited embryo germination of Astragalus differently with the strongest at 25 degrees C, and next to it at 20,30 degrees C. The impact of light on bioactive substances of the toxin was not statistically distinctive, but the 24-hour darkness was benefit to toxin production. PSC had a stronger inhibition rate than the other nutrient media, next to it was PDB. After autoclaving, the toxin still kept toxic to embryo of Astragalus, which indicated that the toxin was tolerant to high temperatures. The toxin produced by F. solani at different growing condition had strong biological activity, was tolerant to high temperature. The best condition for F. solani to produce toxin was that it was cultured in PSC liquid medium, in dark, at 25 degrees C for 12 d. The toxin produced by isolate HQM40 was non-host specific toxin.

  9. Effect of airstream velocity on mean drop diameters of water sprays produced by pressure and air atomizing nozzles

    Ingebo, R. D.


    A scanning radiometer was used to determine the effect of airstream velocity on the mean drop diameter of water sprays produced by pressure atomizing and air atomizing fuel nozzles used in previous combustion studies. Increasing airstream velocity from 23 to 53.4 meters per second reduced the Sauter mean diameter by approximately 50 percent with both types of fuel nozzles. The use of a sonic cup attached to the tip of an air assist nozzle reduced the Sauter mean diameter by approximately 40 percent. Test conditions included airstream velocities of 23 to 53.4 meters per second at 293 K and atmospheric pressure.

  10. Producing intricate IPMC shapes by means of spray-painting and printing (Conference Presentation)

    Trabia, Sarah; Olsen, Zakai; Hwang, Taeseon; Kim, Kwang Jin


    Ionic Polymer-Metal Composites (IPMC) are common soft actuators that are Nafion® based and plated with a conductive metal, such as platinum, gold, or palladium. Nafion® is available in three forms: sheets, pellets, and water dispersion. Nafion® sheets can be cut to the desired dimensions and are best for rectangular IPMCs. However, the user is not able to change the thickness of these sheets by stacking and melting because Nafion® does not melt. A solution to this is Nafion® pellets, which can melt. These can be used for extrusion and injection molding. Though Nafion® pellets can be melted, they are difficult to work with, making the process quite challenging to master. The last form is Nafion® Water Dispersion, which can be used for casting. Casting can produce the desired thickness, but it does not solve the problem of achieving complex contours. The current methods of fabrication do not allow for complex shapes and structures. To solve this problem, two methods are presented: painting and printing. The painting method uses Nafion® Water Dispersion, an airbrush, and vinyl stencils. The stencils can be made into any shape with detailed edges. The printing method uses Nafion® pellets that are extruded into filaments and a commercially available 3D printer. The models are drawn in a Computer-Aided Drawing (CAD) program, such as SolidWorks. The produced Nafion® membranes will be compared with a commercial Nafion® membrane through a variety of tests, including Fourier Transform Infrared Spectroscopy, Scanning Electron Microscope, Thermogravimetric Analysis, Dynamic Mechanical Analysis, and Optical Microscope.


    Valeriy G. Apalkov


    Full Text Available The article covers the key problems of forming cross cultural competence by means of asynchronic Internet-communication techniques. A concise overview of main studies in using e-mail group in forming cross cultural competence. An algorithm of forming cross cultural competence of high school students is described. 

  12. Prevalence of extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated from blood cultures in Africa.

    Sangare, S A; Maiga, A I; Guindo, I; Maiga, A; Camara, N; Savadogo, S; Diallo, S; Bougoudogo, F; Armand-Lefevre, L; Andremont, A; Maiga, I I


    Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae have been isolated from many regions of the world. Epidemiological studies are being conducted in Europe, North America, and Asia. No study has however been conducted in Africa to determine the prevalence and distribution of ESBLs on the continent. This literature review aimed at describing the prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures, as well as the ESBL genes involved at the international level. Our focus was mainly on Africa. We conducted a literature review on PubMed. Articles related to our study field and published between 1996 and 2014 were reviewed and entirely read for most of them, while we only focused on the abstracts of some other articles. Relevant articles to our study were then carefully reviewed and included in the review. The prevalence of ESBL-producing Enterobacteriaceae differs from one country to another. The results of our literature review however indicate that class A ESBLs prevail over the other types. We took into consideration articles focusing on various types of samples to assess the prevalence of ESBL-producing Enterobacteriaceae, but information on isolates from blood cultures is limited. The worldwide prevalence of ESBL-producing Enterobacteriaceae has increased over time. Evidence of ESBL-producing Enterobacteriaceae can be found in all regions of the world. Studies conducted in Africa mainly focused on the Northern and Eastern parts of the continent, while only rare studies were carried out in the rest of the continent.

  13. Active biomonitoring of airborne fluoride near an HF producing factory using standardised grass cultures

    Franzaring, J.; Klumpp, A.; Fangmeier, A.

    In order to study the pollution gradient in the vicinity of an HF producing factory, a biomonitoring programme was performed employing VDI standardised grass cultures. Specimen plants of Lolium multiflorum cv. Lema were exposed at 11 sites over five monthly periods and the biomass produced was used for subsequent F-analyses. Meteorological data from the study region confirmed that wind direction accounted for changes in the pollution pattern over periods of time. Fluoride concentrations in the grass cultures, however, were unrelated to temperature and precipitation sums during the exposures. The biomass production of the grass cultures proved to be unrelated to these parameters as well but, with the enhanced growth of the plants, the fluoride concentrations were lower due to the dilution of the element with higher biomass accumulation. Because the contribution of particulate fluoride was unknown, both the washed grass cultures and the washing water were analysed in order to determine the amount of external fluoride. Washing reduced the fluoride concentrations by 22% on average, indicating that most of the element was internal fluoride stemming from stomatal uptake. Larger amounts of fluoride, however, could be washed off from grass cultures exposed at sites close to the factory indicating that dust emissions played a greater role at these locations. Because particulate emissions were supposed to arise from CaF 2 and the waste-product anhydrite, grass cultures were also analysed for calcium and sulphur. While calcium concentrations were generally high but unrelated to fluoride, sulphur concentrations showed a slight relationship to the F-concentrations determined in the unwashed plants. Latter findings indicate the co-deposition of the two elements as surface bound, external loads, but bioindication could not clarify to what extent both elements were partitioned in the gas-to-particle phase. We therefore recommend using the grass culture method in air quality

  14. (Independent meaning: Representations of addiction and recovery in selected poems produced in a rehabilitation centre

    Antoinette Pretorius


    Full Text Available In this article we provide a close reading of selected poems written during creative writing workshops at a drug rehabilitation centre. We argue that these poems expose some of the uncertainties and complexities that characterise the representation of identity in experiences of addiction and recovery. We show that the speakers in these poems attempt to imagine and represent their experiences in language through a number of structuring binaries. These binaries include those between the speaker’s experiences of active addiction and recovery, and the speaker’s personal experience versus societal expectations and perceptions. Our reading of these poems is informed by the clinical context in which they were written, and our analysis reflects the bifurcation that governs this liminal space. Individual agency in these different spheres is approached in a very tentative way, and the speakers in these poems are shown to have trouble envisioning the future at the same time as their pasts appear unsettled. We argue finally that while current discourses and vocabularies surrounding addiction seem incomplete and inadequate for the expression of some complex experiences, poetry provides a platform that accommodates ambivalence and a multiplicity of meanings.

  15. The language of self, power, meaning: Japanese literature and the cultural boundaries of ideology

    James A. Wren


    Full Text Available To understand the idioms used in representing literary selfhood as enactments and rhetorical assertions is to observe their contextual construction within shifting fields of power and meaning inseparable from the specific and discernible situations, culturally specific conventions affecting their narration, in short, the specificity of historical moment. In the Japanese context, identity is clearly not the unified essence implied by the Cartesian premise, but it can be the presentation or performance of a spatiotemporal locatedness of contradiction and disunity, a nexus wherein multiple discourses temporarily coincide in remarkable and discernible ways we apprehend in the abstracted self. But it is not enough that we as readers celebrate in the particularity of these performance as expressions of what is often dismissed as "local knowledge." Rather, we must question what social institutions--be they alien or indigenous--these performances address. What conventions did they oppose? What apparatus of power does the dialectic of the gaze set itself in opposition? For only by questioning the strategies that erected the facade of boundedness in general and one in particular that created the illusions of insulating the self from the play of power relations in specific works of fictional self-representation can we hope to force a larger reconsideration of selves and their pathways to emergence. In many ways, my task as a member of a particular reading audience has been and will always be to visualize the overall impact of cultural forces on patterns of fictional self-representation and to accept that identity is negotiated, open, shifting, ambiguous, the result of culturally available meanings and the open-ended, power-laden enactments of these meanings in everyday situations.

  16. Parental grief and memento mori photography: narrative, meaning, culture, and context.

    Blood, Cybele; Cacciatore, Joanne


    Postmortem photography is a widespread practice in perinatal bereavement care, yet few studies have explored how it affects bereaved parents, or how it might be received by parents of older children. This study is an examination of the meaning, utility, and social context of postmortem photography in a sample of 181 bereaved parents. Data were subjected to both quantitative and qualitative analysis. Photographs were positively regarded by most parents after perinatal death and several parents of older children. Other parents rejected postmortem photography for aesthetic, personal, or cultural reasons. Brief recommendations are offered for healthcare providers.

  17. The romance with personal meaning in gerontology: cultural aspects of life themes.

    Luborsky, M R


    Life narratives have wide appeal because they promote the modern ideal of freeing people to reflect on their life and to share personal meanings and experience in public. Constructing a life story may aid adult development and well-being. This article explores how to enhance these benefits, but criticizes the idealization of stories and their benefits. Using life stories from 16 randomly selected clinically depressed and nondepressed elderly persons, this article examines the narrative structures and how these relate to mood and to ideal images for the self. Findings show that some normative styles for self-representation (i.e., personal themes) serve to express distress instead of positive well-being. The discussion points to cultural and historical forces shaping our ideas about themes in stories. Life stories do privilege personal meanings, but the settings and form constrain their value as research data and their effectiveness as vehicles for enhancing well-being.

  18. Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures.

    Pasteran, Fernando; Tijet, Nathalie; Melano, Roberto G; Corso, Alejandra


    We compared carbapenemase detection among 266 Gram-negative bacilli (161 carbapenemase producers) using the Carba NP tests issued by the CLSI (CNPt-CLSI) and a novel protocol (CNPt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the CLSI tests). The specificities were comparable (100%), but the CNPt-direct was more sensitive (98% versus 84%). The CNPt-direct was easier to perform due to the direct use of colonies and offered a more robust detection of carbapenemase producers. Copyright © 2015, American Society for Microbiology. All Rights Reserved.


    Hermanto Hermanto


    Full Text Available Writing is a skill derived from a long way of learning and exercises. Different from other language skills, writing is considered the difficult language skill to acquire since it involves many aspects of linguistics, social, and writing knowledge and conventions. There are at least three important elements of writing useful to produce a good piece of composition, language competence, writing competence and cultural competence. This paper shows the influence of these three elements in order to produce good, readable, communicative, and successful writing

  20. Population size and cultural evolution in nonindustrial food-producing societies.

    Mark Collard

    Full Text Available Modeling work suggests that population size affects cultural evolution such that larger populations can be expected to have richer and more complex cultural repertoires than smaller populations. Empirical tests of this hypothesis, however, have yielded conflicting results. Here, we report a study in which we investigated whether the subsistence toolkits of small-scale food-producers are influenced by population size in the manner the hypothesis predicts. We applied simple linear and standard multiple regression analysis to data from 40 nonindustrial farming and pastoralist groups to test the hypothesis. Results were consistent with predictions of the hypothesis: both the richness and the complexity of the toolkits of the food-producers were positively and significantly influenced by population size in the simple linear regression analyses. The multiple regression analyses demonstrated that these relationships are independent of the effects of risk of resource failure, which is the other main factor that has been found to influence toolkit richness and complexity in nonindustrial groups. Thus, our study strongly suggests that population size influences cultural evolution in nonindustrial food-producing populations.

  1. The role of acid incubation in rapid immobilization of hydrogen-producing culture in anaerobic upflow column reactors

    Zhang, Zhen-Peng; Tay, Joo-Hwa [School of Civil and Environmental Engineering, Nanyang Technological University (Singapore); Institute of Environmental Science and Engineering, Nanyang Technological University (Singapore); Show, Kuan-Yeow [Faculty of Science, Engineering and Technology, University Tunku Abdul Rahman, 31900 Kampar, Perak (Malaysia); Liang, David Tee [Institute of Environmental Science and Engineering, Nanyang Technological University (Singapore); Lee, Duu-Jong [Department of Chemical Engineering, National Taiwan University, Taipei 10617 (China); Su, Ay [Department of Mechanical Engineering, Fuel Cell Center, Yuan-Ze University, Taoyuan 320 (China)


    An approach of acidification was examined on formation of hydrogen-producing granules and biofilms in upflow column-shaped reactors. The reactors were fed with synthetic glucose wastewater and operated at 37 C and pH 5.5. The acclimated anaerobic culture was inoculated in four reactors designated R1, R2, R3 and R4, with R3 and R4 filled with granular activated carbon as support medium. To unveil the roles of acidification, microbial culture in R2 and R3 was subject to an acid incubation for 24 h by shifting the culture pH from 5.5 to 2.0. The experimental results suggested that the acidification substantially accelerated microbial granulation, but not biofilm formation. Microbial activities were inhibited by the acid incubation for about 78 h, resulting in the retarded formation of biofilms of the acidified culture. Reducing culture pH resulted in improvement in cell surface physicochemical properties favoring microbial adhesion and immobilization. Zeta potential increased from -25.3 mV to 11.9 mV, hydrophobicity in terms of contact angle improved from 31 to 38 and production of extracellular polymers increased from 66 mg/g-VSS to 136 mg/g-VSS. As a result of the formation of granules and biofilms, high hydrogen production rates of 6.98 and 7.49 L/L h were achieved in granule-based and biofilm-based reactors, respectively. It is concluded that acid incubation is an efficient means to initiate the rapid formation of granules by regulating the surface characteristics of microbial culture. The use of support media as starting nuclei may result in rapid formation of biofilms without the acidification. (author)

  2. Living between two worlds: two-phase culture systems for producing plant secondary metabolites.

    Malik, Sonia; Hossein Mirjalili, Mohammad; Fett-Neto, Arthur Germano; Mazzafera, Paulo; Bonfill, Mercedes


    The two-phase culture system is an important in vitro strategy to increase the production of secondary metabolites (SMs) by providing an enhanced release of these compounds from plant cells. Whereas the first phase supports cell growth, the second phase provides an additional site or acts as a metabolic sink for the accumulation of SMs and also reduces feedback inhibition. This review is focused on several aspects of the two-phase culture system and aims to show the diverse possibilities of employing this technique for the in vitro production of SMs from plant cells. Depending on the material used in the secondary phase, two-phase culture systems can be broadly categorised as liquid-liquid or liquid-solid. The choice of material for the second phase depends on the type of compound to be recovered and the compatibility with the other phase. Different factors affecting the efficiency of two-phase culture systems include the choice of material for the secondary phase, its concentration, volume, and time of addition. Factors such as cell elicitation, immobilization, and permeabilization, have been suggested as important strategies to make the two-phase culture system practically reliable on a commercial scale. Since there are many possibilities for designing a two-phase system, more detailed studies are needed to broaden the range of secondary phases compatible with the various plant species producing SMs with potential applications, mainly in the food and pharmacology industries.

  3. Pollen and Anther Cultures as Potential Means in Production of Haploid Kenaf (Hibiscus cannabinus L.

    Dwi Susanto


    Full Text Available Kenaf (Hibiscus cannabinus L. is one of the important species of Hibiscus for human needs. It is annual upright plant that produces high biomass, has good adaptability to its environment and inexpensive to be grown. Kenaf has been cultivated in many countries since long time ago mainly for fibers. In Malaysia, however, this plant is considered new and cultivated especially in the eastern parts of Peninsular to replace tobacco plantations that have been no longer supported by the government. This plant has potential to be bred to improve the quality and the adaptability. One of the potential breeding methods to improve the plant is by the production of hybrid varieties. These varieties are superior over the inbred varieties because of the heterosis effect (hybrid vigor ofF1heterozygous. Hybrid varieties can be produced by reciprocal combinations of crosses among homozygous lines. Homozygous lines conventionally can bachieved by repeated selfing till seven or more generations that need a lot of times. The production of haploid plants in vitro followed by chromosome doubling is a ‘short-cut’ method to produce homozygous lines in relatively short time compared to conventional methods. The objective of this study is to produce haploid lines through pollen and anther cultures in vitro. The population of haploid plantlets from this research will be induced for diploidization to produce homozygous-heterogeneous population as a basis for the production of hybrid varieties. Preliminary result showed that callus can easily be induced from the anthers compared those from the pollen grains.

  4. Short- and long-term temperature effects on aerobic polyhydroxybutyrate producing mixed cultures.

    Johnson, Katja; van Geest, Jorien; Kleerebezem, Robbert; van Loosdrecht, Mark C M


    Short- and long-term temperature effects on polyhydroxybutyrate (PHB) producing mixed cultures enriched in feast-famine sequencing batch reactors (SBRs) were investigated in a temperature range of 15-35 degrees C and 15-30 degrees C, respectively. After short-term temperature changes (i.e. 1 cycle) from the steady state temperature of 20 degrees C, reaction rate changes in the famine phase could be described over the whole temperature range with the Arrhenius equation with one temperature coefficient. For the feast phase different temperature coefficients were identified for acetate uptake, PHB production and growth. These were only valid for temperatures 5 degrees C higher or lower than the steady state temperature. Long-term temperature changes (i.e. new steady states) influenced not only the reaction rates but also the selective pressure in the SBR. At higher temperatures (30 degrees C) the SBR feast phase was short and the rates of acetate uptake and PHB storage were very high. This culture was characterized by a storage strategy with high yields of PHB and low yields of biomass in the feast phase. The PHB storage capacity of this culture was 84 wt% as evaluated in fed-batch experiments. At lower temperatures (15 degrees C) the feast phase was longer due to a lower rate of acetate uptake and the culture followed a strategy of direct growth on acetate rather than on PHB. This culture had a low maximal PHB storage capacity (about 35 wt%). The SBR culture enriched at 20 degrees C was able to store up to about 70 wt% PHB. The temperature at which fed-batch experiments were conducted did not influence the maximal PHB storage capacity. The SBR temperature was found to be an important factor to consider when designing a mixed culture PHB production process.

  5. Hepatitis E Virus Produced from Cell Culture Has a Lipid Envelope.

    Ying Qi

    Full Text Available The absence of a productive cell culture system hampered detailed analysis of the structure and protein composition of the hepatitis E virion. In this study, hepatitis E virus from a robust HEV cell culture system and from the feces of infected monkeys at the peak of virus excretion was purified by ultra-centrifugation. The common feature of the two samples after ultracentrifugation was that the ORF2 protein mainly remained in the top fractions. The ORF2 protein from cell culture system was glycosylated, with an apparent molecular weight of 88 kDa, and was not infectious in PLC/PRF/5 cells. The ORF2 protein in this fraction can bind to and protect HEV RNA from digestion by RNase A. The RNA-ORF2 product has a similar sedimentation coefficient to the virus from feces. The viral RNA in the cell culture supernatant was mainly in the fraction of 1.15 g/cm3 but that from the feces was mainly in the fraction of 1.21 g/cm3. Both were infectious in PLC/PRF/5 cells. And the fraction in the middle of the gradient (1.06 g/cm3 from the cell culture supernatant,but not that from the feces, also has ORF2 protein and HEV RNA but was not infectious in PLC/PRF/5.The infectious RNA-rich fraction from the cell culture contained ORF3 protein and lipid but the corresponding fraction from feces had no lipid and little ORF3 protein. The lipid on the surface of the virus has no effect on its binding to cells but the ORF3 protein interferes with binding. The result suggests that most of the secreted ORF2 protein is not associated with HEV RNA and that hepatitis E virus produced in cell culture differs in structure from the virus found in feces in that it has a lipid envelope.

  6. Metabolic analysis of antibody producing Chinese hamster ovary cell culture under different stresses conditions.

    Badsha, Md Bahadur; Kurata, Hiroyuki; Onitsuka, Masayoshi; Oga, Takushi; Omasa, Takeshi


    Chinese hamster ovary (CHO) cells are commonly used as the host cell lines concerning their ability to produce therapeutic proteins with complex post-translational modifications. In this study, we have investigated the time course extra- and intracellular metabolome data of the CHO-K1 cell line, under a control and stress conditions. The addition of NaCl and trehalose greatly suppressed cell growth, where the maximum viable cell density of NaCl and trehalose cultures were 2.2-fold and 2.8-fold less than that of a control culture. Contrariwise, the antibody production of both the NaCl and trehalose cultures was sustained for a longer time to surpass that of the control culture. The NaCl and trehalose cultures showed relatively similar dynamics of cell growth, antibody production, and substrate/product concentrations, while they indicated different dynamics from the control culture. The principal component analysis of extra- and intracellular metabolome dynamics indicated that their dynamic behaviors were consistent with biological functions. The qualitative pattern matching classification and hierarchical clustering analyses for the intracellular metabolome identified the metabolite clusters whose dynamic behaviors depend on NaCl and trehalose. The volcano plot revealed several reporter metabolites whose dynamics greatly change between in the NaCl and trehalose cultures. The elastic net identified some critical, intracellular metabolites that are distinct between the NaCl and trehalose. While a relatively small number of intracellular metabolites related to the cell growth, glucose, glutamine, lactate and ammonium ion concentrations, the mechanism of antibody production was suggested to be very complicated or not to be explained by elastic net regression analysis. Copyright © 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  7. Flow cytometric viability assessment of lactic acid bacteria starter cultures produced by fluidized bed drying.

    Bensch, Gerald; Rüger, Marc; Wassermann, Magdalena; Weinholz, Susann; Reichl, Udo; Cordes, Christiana


    For starter culture production, fluidized bed drying is an efficient and cost-effective alternative to the most frequently used freeze drying method. However, fluidized bed drying also poses damaging or lethal stress to bacteria. Therefore, investigation of impact of process variables and conditions on viability of starter cultures produced by fluidized bed drying is of major interest. Viability of bacteria is most frequently assessed by plate counting. While reproductive growth of cells can be characterized by the number of colony-forming units, it cannot provide the number of viable-but-nonculturable cells. However, in starter cultures, these cells still contribute to the fermentation during food production. In this study, flow cytometry was applied to assess viability of Lactobacillus plantarum starter cultures by membrane integrity analysis using SYBR®Green I and propidium iodide staining. The enumeration method established allowed for rapid, precise and sensitive determination of viable cell concentration, and was used to investigate effects of fluidized bed drying and storage on viability of L. plantarum. Drying caused substantial membrane damage on cells, most likely due to dehydration and oxidative stress. Nevertheless, high bacterial survival rates were obtained, and granulates contained in the average 2.7 × 10(9) viable cells/g. Furthermore, increased temperatures reduced viability of bacteria during storage. Differences in results of flow cytometry and plate counting suggested an occurrence of viable-but-nonculturable cells during storage. Overall, flow cytometric viability assessment is highly feasible for rapid routine in-process control in production of L. plantarum starter cultures, produced by fluidized bed drying.

  8. Profile of the culturable microbiome capable of producing acyl-homoserine lactone in the tobacco phyllosphere

    Di Lv; Anzhou Ma; Xuanming Tang; Zhihui Bai; Hongyan Qi; Guoqiang Zhuang


    Bacterial populations coexisting in the phyllosphere niche have important effects on plant health.Quorum sensing (QS) allows bacteria to communicate via diffusible signal molecules,but QS-dependent behaviors in phyllosphere bacterial populations are poorly understood.We investigate the dense and diverse N-acyl-homoserine lactone (AHL)-producing phyllosphere bacteria living on tobacco leaf surfaces via a culture-dependent method and 16S rRNA gene sequencing.Our results indicated that approximately 7.9%-11.7%of the culturable leaf-associated bacteria have the ability to produce AHL based on the assays using whole-cell biosensors.Sequencing of the 16S rRNA gene assigned the AHL-producing strains to two phylogenetic groups,with Gammaproteobacteria (93%) as the predominant group,followed by Alphaproteobacteria.All of the AHL-producing Alphaproteobacteria were affiliated with the genus Rhizobium,whereas the AHL-producing bacteria belonging to the Gammaproteobacteria mainly fell within the genera Pseudomonas,Acinetobacter,Citrobacter,Enterobacter,Pantoea and Serratia.The bioassays of supernatant extracts revealed that a portion of the strains have a remarkable AHL profile for AHL induction activity using the two different biosensors,and one compound in the active extract of a representative isolate,NTL223,corresponded to 3-oxo-hexanoyl-homoserine lactone.A large population size and diversity of bacteria capable of AHL-driven QS were found to cohabit on leaves,implying that cross-communication based AHL-type QS may be common in the phyllosphere.Furthermore,this study provides a general snapshot of a potential valuable application of AHL-producing bacteria inhabiting leaves for their presumable ecological roles in the phyllosphere.

  9. Ex Vivo Produced Oral Mucosa Equivalent by Using the Direct Explant Cell Culture Technique

    Kamile Öztürk


    Full Text Available Objective: The aim of this study is the histological and immunohistochemical evaluation of ex vivo produced oral mucosal equivalents using keratinocytes cultured by direct explant technique.Material and Methods: Oral mucosa tissue samples were obtained from the keratinized gingival tissues of 14 healthy human subjects. Human oral mucosa keratinocytes from an oral mucosa biopsy specimen were dissociated by the explant technique. Once a sufficient population of keratinocytes was reached, they were seeded onto the type IV collagen coated “AlloDerm” and taken for histological and immunohistochemical examinations at 11 days postseeding of the keratinocytes on the cadaveric human dermal matrix.Results: Histopathologically and immunohistochemically, 12 out of 14 successful ex vivo produced oral mucosa equivalents (EVPOME that consisted of a stratified epidermis on a dermal matrix have been developed with keratinocytes cultured by the explant technique.Conclusion: The technical handling involved in the direct explant method at the beginning of the process has fewer steps than the enzymatic method and use of the direct explant technique protocol for culturing of human oral mucosa keratinocyte may be more adequate for EVPOME production.

  10. Physico-chemical properties of polyhydroxyalkanoate produced by mixed-culture nitrogen-fixing bacteria.

    Patel, Meeta; Gapes, Daniel J; Newman, Roger H; Dare, Peter H


    Ultra-high molecular weight polyhydroxyalkanoates (PHAs) with low polydispersity index (PDI = 1.3) were produced in a novel, pilot scale application of mixed cultures of nitrogen-fixing bacteria. The number average molecular weight (M (n)) of the poly(3-hydroxybutyrate) (P(3HB)) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) was determined to be 2.4 x 10(6) and 2.5 x 10(6) g mol(-1), respectively. Using two types of carbon sources, biomass contents of the P(3HB) and P(3HB-co-3HV) were 18% and 30% (PHA in dry biomass), respectively. The extracted polymers were analysed for their physical properties using analytical techniques such as nuclear magnetic resonance (NMR) spectroscopy, differential scanning calorimetry (DSC) and gel permeation chromatography (GPC). NMR confirmed the formation of homopolymer and copolymer. DSC showed a single melting endotherm peak for both polymers, with enthalpies that indicated crystallinity indices of 44% and 37% for P(3HB) and P(3HB-co-3HV), respectively. GPC showed a sharp unimodal trace for both polymers, reflecting the homogeneity of the polymer chains. The work described here emphasises the potential of mixed colony nitrogen-fixing bacteria cultures for producing biodegradable polymers which have properties that are very similar to those from their pure-culture counterparts and therefore making a more economically viable route for obtaining biopolyesters.

  11. Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells.

    De Alwis, Ransika; Fujita, Koki; Ashitani, Tatsuya; Kuroda, Ken'ichi


    Elicitor treatment initiates defense responses in cultured Cupressus lusitanica cells. In order to investigate the defense mechanism with a yeast extract elicitor, we carried out solid-phase microextraction coupled with gas chromatography for monoterpene analysis. Ten hydrocarbon monoterpenes, including high amounts of sabinene and limonene, were detected in the gas phase of the elicitor-treated cell cultures. Six oxidized monoterpenes including beta-thujaplicin were also detected in the ether extract of the cells and the medium. Time-course profiles of volatile monoterpenes showed that one group of hydrocarbon monoterpenes was maximized on the second day after elicitation, while the other group was maximized on the third day. There were no oxidized monoterpenes that are structurally related to sabinene and limonene in the gas phase or cell extracts, suggesting that these compounds are produced exclusively for emission. Other monoterpenes, which are produced during later stages of elicitation, are metabolized into more complex compounds such as oxidized monoterpenes, including beta-thujaplicin. Although terpinolene synthase was the principal monoterpene synthase in these cell cultures, terpinolene was detected only as a minor compound in the gas phase. The time course for terpinolene synthase activity coincided with beta-thujaplicin biosynthesis. Thus, most of the terpinolene is metabolized rapidly to oxidized terpenes such as beta-thujaplicin rather than emitted.

  12. L-ergothioneine supplementation during culture improves quality of bovine in vitro-produced embryos.

    Zullo, G; Albero, G; Neglia, G; De Canditiis, C; Bifulco, G; Campanile, G; Gasparrini, B


    The aim of this study was to evaluate whether supplementation of bovine culture medium with the natural antioxidant L-ergothioneine (LE), improves in vitro blastocyst development and quality, assessed as resistance to cryopreservation, total cells number, cellular differentiation, and apoptosis index. Abattoir-derived oocytes were matured and fertilized in vitro according to standard procedure. Twenty hours after IVF, presumptive zygotes were cultured in synthetic oviduct fluid with 0, 0.05 mM, 0.1 mM, 0.5 mM, and 1 mM of LE (experiment 1) at 39 °C under humidified air with 5% CO2, 7% O2, and 88% N2. On the basis of the results of this dose-response trial, the range of concentrations to test was reduced in experiment 2, in which presumptive zygotes were cultured with 0, 0.05 mM, and 0.1 mM of LE. On Day 7, embryo yields were assessed, and the blastocysts (BL) were vitrified by Cryotop method in 16.5% ethylene glycol, 16.5% DMSO and 0.5 M sucrose. Finally, BL produced on Day 8 in the absence (control) and presence of 0.1 mM LE were used for transferase-mediated dUTP nick end labeling and differential staining to evaluate, respectively the apoptotic rate and the allocation of cells into inner cell mass (ICM) and trophectoderm lineages (experiment 3). Despite similar blastocyst yields, supplementation of culture medium with 0.1 mM LE improved the cryotolerance of in vitro-produced (IVP) embryos compared to the control group, as indicated by higher (P culture (48.5%, 50.0%, and 63.8%, respectively with 0, 0.05, and 0.1 mM LE). Interestingly, when embryos were cultured in the presence of 0.1 mM LE, the percentage of BL with the most physiological ICM:total cells ratio (20%-40%) increased (85.1 vs. 66.0%, P culture medium with 0.1 mM LE improves embryo quality, as indicated by the improved cryotolerance, the lower apoptotic rate, and the higher percentage of BL with the most physiological ICM:total cells ratio.

  13. Process for the lysis of a culture of lactic acid bacteria by means of a lysin, and uses of the resulting lysed culture

    Buist, Girbe; Venema, Gerard; Kok, Jan; Ledeboer, Aat M.


    The invention provides a process for the lysis of a culture of lactic acid bacteria, or a product containing such culture e.g. cheese, by means of a lysin through the in situ production of a homologous autolysin, or a heterologous autolysin obtainable from Gram-positive bacteria esp. from lactic aci

  14. Antiviral activity produced by an IPNV-carrier EPC cell culture confers resistance to VHSV infection.

    Jurado, María Teresa; García-Valtanen, Pablo; Estepa, Amparo; Perez, Luis


    Infectious pancreatic necrosis virus (IPNV), a fish birnavirus, can establish a persistent infection on epithelioma papulosum cyprinid (EPC) cells producing a carrier state where a small fraction of IPNV-infected cells is maintained in the culture after continuous subculture. The EPC(IPNV) cells are resistant to challenge with IPNV as well as to challenge with viral hemorrhagic septicemia virus (VHSV), a rhabdovirus. In this work, the antiviral effect of the IPNV carrier culture conditioned medium (EPC(IPNV)-CM) was tested and analyzed in detail. EPC cells treated with the carrier culture supernatant become protected against VHSV challenge. Size-fractionation by filtration and acid and heat treatment showed that the IPNV persistently infected cells release an acid-resistant soluble factor in the molecular weight fraction bellow 50 kDa. The capacity of the EPC(IPNV)-CM to induce cytokine genes in EPC cells was also determined by real-time RT-PCR. We found that there is a positive correlation between up-regulation of mx gene expression in EPC cells treated with EPC(IPNV)-CM and protection against VHSV challenge. Our findings indicate that the control of IPNV multiplication in the carrier culture as well as the interference with rhabdovirus replication are connected to the production and release of an antiviral (interferon-like) factor to the medium.


    T. Ahmed and R. Kanwal


    Lactic acid bacteria (LAB) were isolated from camel milk by culturing the milk on specific media and pure culture was obtained by sub-culturing. Purification of culture was confirmed by Gram’s staining and identified by different biochemical tests. Camel milk contained lactic acid producing bacteria like Streptococci such as S. cremoris and S. lactis and Lactobacilli such as L. acidophilus. L. acidophilus grew more rapidly in camel milk than others as its growth was supported by camel milk...

  16. Simple screening method for molds producing intracellular mycotoxins in pure cultures.

    Filtenborg, O; Frisvad, J C; Svendsen, J A


    A simple screening method for molds producing the intracellular mycotoxins brevianamide A, citreoviridin, cyclopiazonic acid, luteoskyrin, penitrem A, roquefortine C, sterigmatocystin, verruculogen, viomellein, and xanthomegnin was developed. After removing an agar plug from the mold culture, the mycelium on the plug is wetted with a drop of methanol-chloroform (1:2). By this treatment the intracellular mycotoxins are extracted within seconds and transferred directly to a thin-layer chromatography plate by immediately placing the plug on the plate while the mycelium is still wet. After removal of the plug, known thin-layer chromatographic procedures are carried out. The substrate (Czapek yeast autolysate agar) and growth conditions (25 degrees C for 7 days) used by Penicillium taxonomists proved suitable for the production of the mycotoxins investigated when 60 known toxigenic isolates and 865 cultures isolated from foods and feedstuffs were tested with this screening method.

  17. Significados culturais da asma infantil Cultural meanings of the infantile asthma

    Mirna Albuquerque Frota


    Full Text Available OBJETIVO: Analisar os significados culturais da asma infantil com origem na mãe-cuidadora. PROCEDIMENTOS METODOLÓGICOS: Pesquisa qualitativa realizada em 2004, no município de Fortaleza, CE. Foi utilizada a observação participante com abordagem etnográfica e entrevistas com sete mães acompanhantes de seus filhos em unidade de emergência hospitalar. Os significados das falas das mães foram identificados utilizando-se a técnica de análise temática. ANÁLISE DOS RESULTADOS: Foram identificadas duas categorias de discussão; na primeira, "desinformação sobre a doença", foi possível perceber que as mães não são informadas sobre a doença do filho. Na segunda categoria, "cuidado cultural", as mães referem informações sobre os cuidados e utilizam recursos do saber popular para prevenir a asma dos filhos, como o cuidado ambiental e a utilização de remédios caseiros à base de plantas medicinais. CONCLUSÕES: As características de desinformação e desconhecimento materno em relação à asma do filho mostram a necessidade de haver um trabalho educativo intenso, dialógico e problematizador em estreita colaboração com o tratamento, visando à melhoria do prognóstico da doença.OBJECTIVE: To analyze the cultural meanings of infantile asthma from the perspective of the mother/carer of the child. METHODOLOGICAL PROCEDURES: Qualitative research conducted in 2004, in the city of Fortaleza (Northeastern Brazil. An ethnographic approach was utilized, consisting of participant observation and interviews with seven mothers, accompanying their children in a hospital emergency ward. Thematic analysis was the technique employed when identifying the meanings of mothers' discourse. ANALYSIS OF RESULTS: Two discussion categories were identified: "disinformation on illness" in which it was possible to perceive that the mothers were not informed with respect to their children's illness; and "cultural care" in which they relate information on


    Konakova O.Y.


    Full Text Available Purpose: to find influence of physical culture innovative means (combination of Pilates - exercises for body and mind - and program Port de bras (movements of arms and body on functional state of second maturity women. Material: 22 women of age from 50 to 55 were tested; they were divided into main and control groups. Control group practiced health related swimming; experimental group was trained by system Pilates and program Port de bras with application of special equipment. Results: it was found, that for confident changes in women’s functional systems it was necessary to practice trainings in zone of sub-maximal load (75-85% from maximal oxygen consumption; trainings in zone of low intensity do not give confident health related effect. Conclusions: At low and below average women’s physical fitness it is necessary to practice health related trainings in zone of low intensity (mean heart beats rate -90-115 beats per minute. For increasing trainings intensity it is necessary to raise women’s physical fitness.

  19. Using Pictures as Cultural Means in Finding Similarities between Solutions to Algebraic Tasks

    Kotov A.A.,


    Full Text Available Solving textual algebraic tasks requires finding important mathematical variables in the text and turning them into equations. Many students, following their teacher’s advice or at their own initiative, use pictures as a means of better understanding the mathematical structure of the task. In this study we explored which features of pictures were the most essential to the students in finding the appropriate solution based on their experience. We created a set of pictures that differed in parameters of analogy and completeness of relationships between elements. The subjects were asked to evaluate the usefulness of the solution to the initial task (represented by the picture for solving the target task. As it was revealed, in their evaluations the subjects were more likely to focus on the completeness of the depicted relationships rather than on the analogy of the picture. This tendency was also more evident in experienced subjects as compared to inexpe- rienced ones. The outcomes of our study are interpreted in terms of the development of cultural means in the transition from everyday concepts to scientific concepts in the process of learning.

  20. Cultural Meaning of Perceived Control: A Meta-Analysis of Locus of Control and Psychological Symptoms across 18 Cultural Regions

    Cheng, Cecilia; Cheung, Shu-fai; Chio, Jasmine Hin-man; Chan, Man-pui Sally


    Integrating more than 40 years of studies on locus of control (LOC), this meta-analysis investigated whether (a) the magnitude of the relationship between LOC and psychological symptoms differed among cultures with distinct individualist orientations and (b) depression and anxiety symptoms yielded different patterns of cultural findings with LOC.…

  1. Functional Curriculum Development: A Means of Retaining Nomadic Fulbe Cultural Identity. Contribution of Education to Cultural Development.

    Ezeomah, Chimah

    To develop a functional curriculum for Nigeria's nomadic Fulbe tribespeople it is necessary to understand the cultural setting. The myths of the Fulbe, such as the story of herdsman Sile Sajo's encounter with the deity Kumen, provide insight into the culture. The story reflects the society's agricultural base, identifies personal characteristics…

  2. Cell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens using an internal membrane filtration system.

    Lee, Pyung-Cheon; Lee, Sang-Yup; Chang, Ho-Nam


    Cell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically carried out using an internal membrane filter module in order to examine the physiological response of A. succiniciproducens to a high-cell-density environment. The optimal growth of A. succiniciproducens and its enhanced succinic acid productivity were observed under CO2-rich conditions, established by adding NaHCO3 and Na2CO3, in the cell recycled system. A. succiniciproducens grew up to 6.50 g-DCW/l, the highest cell concentration obtained so far, in cell recycled cultures. The cells did not change their morphology, which is known to be easily changed in unfavorable or stress environments. The maximum productivity of succinic acid was about 3.3 g/l/h, which is 3.3 times higher than those obtained in batch cultures. These results can serve as a guide for designing highly efficient cell recycled systems for succinic acid at a commercial level.

  3. Developing a thermophilic hydrogen-producing co-culture for efficient utilization of mixed sugars

    Zeidan, Ahmad A.; Van Niel, Ed W.J. [Department of Applied Microbiology, Lund University, P.O. Box 124, SE-221 00 Lund (Sweden)


    Previous studies on the extreme thermophile Caldicellulosiruptor saccharolyticus revealed that the organism produces high yields of hydrogen on glucose and xylose, the major components of lignocellulosic hydrolysates. Preliminary experiments on mixed sugar substrates, however, indicated that xylose was preferred over glucose. The sugar preference of some other extreme thermophiles, including Caldicellulosiruptor owensensis, Caldicellulosiruptor kristjanssonii and newly enriched, thermophilic compost sludge microflora, was investigated in an attempt to find complementary organisms to C. saccharolyticus for rapid and efficient utilization of lignocellulosic sugars. The behavior of C. owensensis and C. kristjanssonii appeared to be similar to that of C. saccharolyticus, either in pure cultures or in co-cultures with the latter. Co-culturing C. saccharolyticus with the enriched compost microflora resulted in fast, simultaneous consumption of both glucose and xylose in the medium with a relatively high specific hydrogen production rate, 40 mmol (gCDW){sup -1} h{sup -1}, and high volumetric productivity, 22.5 mmol l{sup -1} h{sup -1}. (author)

  4. Lipolytic Changes in Fermented Sausages Produced with Turkey Meat: Effects of Starter Culture and Heat Treatment.

    Karsloğlu, Betül; Çiçek, Ümran Ensoy; Kolsarici, Nuray; Candoğan, Kezban


    In this study, the effects of two different commercial starter culture mixes and processing methodologies (traditional and heat process) on the lipolytic changes of fermented sausages manufactured with turkey meat were evaluated during processing stages and storage. Free fatty acid (FFA) value increased with fermentation and during storage over 120 d in all fermented sausage groups produced with both processing methodologies (pfermented sausages were between 10.54-13.01% and 6.56-8.49%, respectively. Thiobarbituric acid (TBA) values of traditionally processed fermented sausages were between 0.220-0.450 mg·kg(-1), and TBA values of heat processed fermented sausages were in a range of 0.405-0.795 mg·kg(-1). Oleic and linoleic acids were predominant fatty acids in all fermented sausages. It was seen that fermented sausage groups produced with starter culture had lower TBA and FFA values in comparison with the control groups, and heat application inhibited the lipase enzyme activity and had an improving effect on lipid oxidation. As a result of these effects, heat processed fermented sausages had lower FFA and higher TBA values than the traditionally processed groups.

  5. Between meaning culture and presence effects: contemporary biomedical objects as a challenge to museums.

    Söderqvist, Thomas; Bencard, Adam; Mordhorst, Camilla


    The acquisition and display of material artefacts is the raison d'être of museums. But what constitutes a museum artefact? Contemporary medicine (biomedicine) is increasingly producing artefacts that do not fit the traditional museological understanding of what constitutes a material, tangible artefact. Museums today are therefore caught in a paradox. On the one hand, medical science and technologies are having an increasing pervasive impact on the way contemporary life is lived and understood and is therefore a central part of the contemporary world. On the other hand, the objects involved in medical diagnostics and therapies are becoming increasingly invisible and intangible and therefore seem to have no role to play as artefacts in a museum context. Consequently, museums are at risk of becoming alienated from an increasingly important part of contemporary society. This essay elaborates the paradox by employing Gumbrecht's (2004) distinction between 'presence' and 'meaning'.

  6. 3 dimensional cell cultures: a comparison between manually and automatically produced alginate beads.

    Lehmann, R; Gallert, C; Roddelkopf, T; Junginger, S; Wree, A; Thurow, K


    Cancer diseases are a common problem of the population caused by age and increased harmful environmental influences. Herein, new therapeutic strategies and compound screenings are necessary. The regular 2D cultivation has to be replaced by three dimensional cell culturing (3D) for better simulation of in vivo conditions. The 3D cultivation with alginate matrix is an appropriate method for encapsulate cells to form cancer constructs. The automated manufacturing of alginate beads might be an ultimate method for large-scaled manufacturing constructs similar to cancer tissue. The aim of this study was the integration of full automated systems for the production, cultivation and screening of 3D cell cultures. We compared the automated methods with the regular manual processes. Furthermore, we investigated the influence of antibiotics on these 3D cell culture systems. The alginate beads were formed by automated and manual procedures. The automated steps were processes by the Biomek(®) Cell Workstation (celisca, Rostock, Germany). The proliferation and toxicity were manually and automatically evaluated at day 14 and 35 of cultivation. The results visualized an accumulation and expansion of cell aggregates over the period of incubation. However, the proliferation and toxicity were faintly and partly significantly decreased on day 35 compared to day 14. The comparison of the manual and automated methods displayed similar results. We conclude that the manual production process could be replaced by the automation. Using automation, 3D cell cultures can be produced in industrial scale and improve the drug development and screening to treat serious illnesses like cancer.

  7. The philosophical and educational potential ecopsychology as means of forming ecological culture of personality

    O. V. Kisyel


    Full Text Available The current ecological crisis is largely caused by the dominance of anthropocentric environmental awareness. To overcome it and move humanity to a model of sustainable social and environmental development required the establishment of environmental consciousness ecocentric type, which is the aim of environmental education, namely, the formation of personality type ecocentric environmental awareness. The purpose of the article is to study the philosophical and educational potential environmental psychology as a means of transformation of personal values and attitudes toward nature. Analysis of the concepts of subjective attitude to nature, subjective perception of the natural world, environmental knowledge, empaurment-method pedagogy. Education for Sustainable Development aims to develop such knowledge, skills and values that will enable people to make individual and collective decisions of local and global nature to improve the quality of life without endangering future generations. The basic methodological principle of environmental psyhopedahohiky is in strict accordance with the environmental education teaching psychological process of environmental awareness. Interaction with nature is a great psychological and pedagogical potential that should be used in the environmental education that will allow him to become a factor in the overall development and personality development. So effectively targeted formation ekospozhyvchoyi culture of all populations requires training of qualified specialists in the field of eco-educational activities and environmental education and its philosophical and educational foundation.

  8. Conflicting cultural perspectives: meanings and experiences of postnatal depression among women in Indian communities.

    Jain, Anita; Levy, David


    A woman's cultural and social context affects her experience of postnatal depression. In this literature review, the authors explore questions regarding normal and abnormal postnatal experiences of Indian women with consideration to cross-cultural perspectives. Although postnatal distress or sadness is recognized among many cultures, it is constructed as a transient state in some cultures and as an illness in others. A major challenge for health care providers in Western countries like the United Kingdom and Australia is to develop culturally sensitive approaches to postnatal care for migrant mothers.

  9. Technical means and system guarantee of museumprotection and inheritance for intangible cultural heritage

    TANG Zhiqiang


    Intangible cultural heritage has a rich connotation of unique thinking way, ethical imaginationand cultural awareness, which is an important sign of national spiritual culture. Possessing the specialvalues of professionalism, permanency, security and openness, with the advantages museums play acentral role in inheriting and rescuing our intangible cultural heritage. Museums have a lot of technicalmeans for museums to protect and inherit intangible cultural heritage, such as scientific research,preservation of material carrier, museum publications, displays on intangible cultural heritage, estab-lishing thematic museums. In intangible cultural heritage, agricultural heritage is the most importantpart. The core content of which is the ideas and methods of harmony between mankind and environ-ment. There is a variety of practices on the protection by China National Agricultural Museum.

  10. Refrigeration provides a simple means to synchronize in vitro cultures of Plasmodium falciparum.

    Yuan, Lili; Hao, Mingming; Wu, Lanou; Zhao, Zhen; Rosenthal, Benjamin M; Li, Xiaomei; He, Yongshu; Sun, Ling; Feng, Guohua; Xiang, Zheng; Cui, Liwang; Yang, Zhaoqing


    Plasmodium falciparum is usually asynchronous during in vitro culture. Highly synchronized cultures of P. falciparum are routinely used in malaria research. Here, we describe a simple synchronization procedure for P. falciparum asexual erythrocytic culture, which involves storage at 4°C for 8-24 h followed by routine culture. When cultures with 27-60% of ring stage were synchronized using this procedure, 70-93% ring stages were obtained after 48 h of culture and relative growth synchrony remained for at least two erythrocytic cycles. To test the suitability of this procedure for subsequent work, drug sensitivity assays were performed using four laboratory strains and four freshly adapted clinical P. falciparum isolates. Parasites synchronized by sorbitol treatment or refrigeration showed similar dose-response curves and comparable IC50 values to four antimalarial drugs. The refrigeration synchronization method is simple, inexpensive, time-saving, and should be especially useful when large numbers of P. falciparum culture are handled.

  11. Leaf and root volatiles produced by tissue cultures of Alpinia zerumbet (pers. Burtt & Smith under the influence of different plant growth regulators

    Cristiane Pimentel Victório


    Full Text Available Volatiles produced by plantlets of Alpinia zerumbet were obtained by means of simultaneous distillation-extraction (SDE. The effects of indole-3-acetic acid, kinetin, thidiazuron and 6-benzylaminopurine on leaf and root volatile composition obtained by tissue cultures were investigated. A higher content of b-pinene and a lower content of sabinene were observed in leaf volatile of plantlets cultured in control, IAA and IAA+ TDZ media, as compared with those of donor plants. In vitro conditions were favorable to increase caryophyllene content. Volatile compounds from the root were characterized mainly by camphene, fenchyl-acetate and bornyl acetate; which constitute about 60% of total volatile.

  12. The properties and acceptability of fresh cheese produced using the mixture probiotic culture

    Rajka Božanić


    Full Text Available Investigation and production of dairy food with probiotic cultures isincreasing due to its health and nutritive benefits. In this paper the probiotic fresh cheese was produced from skim milk samples with 0,1% fat (A and 1,0% fat (B. Fermentation of skim milk samples was conducted at 40°C by 2% addition of DVS-ABT4 mixture probiotic cultures inoculum with selected bacteria (Lactobacillus acidophilus, Bifidobacterium ssp. and Streptococcus thermophilus and without rennet addition. After milk coagulation (about 7-9 hours, the curd was gently cut and drained overnight. Produced of skim milk cheese samples (A had approximately 23.4% total solids, 1.8% fat in total solids and yield about 24.5% (w/v, while the low fat cheese samples (B had 26.2% total solids, 16.2% fat in total solids and yield about 27.3% (w/v. The skim milk cheeses (A had higher protein and mineral matter content compared to low fat cheeses (B. All samples of probiotic fresh cheese were a like to traditional fresh cheese according to general appearance and consistency. However, they had slightly lower aroma and acidity, expecially skim milk cheese samples (A. Better sensory properties had low fat cheesesamples (B during total time of storage (14 days. Acceptability of probiotic fresh cheese was evalueted by hedonic scale from 63 consumers. Statistic shows that all samples were 100% desirable, but average score for skim milk cheese (A was some lower (x = 7.33 than average score (x = 8.11 for low fat cheese (B. Variance analysis also shows that there is significantly important difference (p= 0,05 between analysed fresh cheese samples

  13. Expression System Based on an MTIIa Promoter to Produce hPSA in Mammalian Cell Cultures

    Santos, Anderson K.; Parreira, Ricardo C.; Resende, Rodrigo R.


    Because of the limitations of standard culture techniques, the development of new recombinant protein expression systems with biotechnological potential is a key challenge. Ideally, such systems should be able to effectively and accurately synthesize a protein of interest with intrinsic metabolic capacity. Here, we describe such a system that was designed based on a plasmid vector containing promoter elements derived from the metallothionein MTIIa promoter, as well as processing and purification elements. This promoter can be induced by heavy metals in a culture medium to induce the synthesis of human prostate-specific antigen (hPSA), which has been modified to insert elements for purification, proteolysis, and secretion. We optimized hPSA production in this system by comparing the effects and contributions of ZnCl2, CdCl2, and CuSO4 in HEK293FT, HeLa, BHK-21, and CHO-K1 cells. We also compared the effectiveness of three different transfection agents: multi-walled carbon nanotubes, Lipofectamine 2000, and X-tremeGENE HP Reagent. hPSA production was confirmed via the detection of enhanced green fluorescent protein fluorescence, and cell viability was determined. The expression of hPSA was compared with that of the native protein produced by LNCaP cells, using enzyme-linked immunosorbent assay and sodium dodecyl sulfate polyacrylamide gel electrophoresis. X-tremeGENE reagent, the BHK-21 cell line, and CuSO4 showed the highest hPSA production rates. Furthermore, BHK-21 cells were more resistant to the oxidative stress caused by 100 μM CuSO4. These results suggest that the proposed optimized inducible expression system can effectively produce recombinant proteins with desired characteristics for a wide range of applications in molecular biology. PMID:27582737

  14. Expression system based on an MTIIa promoter to produce hPSA in mammalian cell cultures

    Anderson K Santos


    Full Text Available Because of the limitations of standard culture techniques, the development of new recombinant protein expression systems with biotechnological potential is a key challenge. Ideally, such systems should be able to effectively and accurately synthesize a protein of interest with intrinsic metabolic capacity. Here, we describe such a system that was designed based on a plasmid vector containing promoter elements derived from the metallothionein MTIIa promoter, as well as processing and purification elements. This promoter can be induced by heavy metals in a culture medium to induce the synthesis of human prostate-specific antigen (hPSA, which has been modified to insert elements for purification, proteolysis, and secretion. We optimized hPSA production in this system by comparing the effects and contributions of ZnCl2, CdCl2, and CuSO4 in HEK293FT, HeLa, BHK-21, and CHO-K1 cells. We also compared the effectiveness of three different transfection agents: multi-walled carbon nanotubes, Lipofectamine 2000, and X-tremeGENE HP Reagent. hPSA production was confirmed via the detection of enhanced green fluorescent protein fluorescence, and cell viability was determined. The expression of hPSA was compared with that of the native protein produced by LNCaP cells, using enzyme-linked immunosorbent assay and sodium dodecyl sulphate polyacrylamide gel electrophoresis. X-tremeGENE reagent, the BHK-21 cell line, and CuSO4 showed the highest hPSA production rates. Furthermore, BHK-21 cells were more resistant to the oxidative stress caused by 100 μM CuSO4. These results suggest that the proposed optimized inducible expression system can effectively produce recombinant proteins with desired characteristics for a wide range of applications in molecular biology.

  15. Is the lower atmosphere a readily accessible reservoir of culturable, antimicrobial compound-producing Actinomycetales?

    Carolyn F. Weber


    Full Text Available Recent metagenomic studies have revealed that microbial diversity in the atmosphere rivals that of surface environments. This indicates that the atmosphere may be worth bioprospecting in for novel microorganisms, especially those selected for by harsh atmospheric conditions. This is interesting in light of the antibiotic resistance crisis and renewed interests in bioprospecting for members of the Actinomycetales, which harbor novel secondary metabolite-producing pathways and produce spores that make them well suited for atmospheric travel. The latter leads to the hypothesis that the atmosphere may be a promising environment in which to search for novel Actinomycetales. Although ubiquitous in soils, where bioprospecting efforts for Actinomycetales have been and are largely still focused, we present novel data indicating that culturable members of this taxonomic order are 3 to 5.6 times more abundant in air samples collected at 1.5, 4.5, 7.5 and 18 m above the ground, than in the underlying soil. These results support the hypothesis that mining the vast and readily accessible lower atmosphere for novel Actinomycetales in the search for undescribed secondary metabolites, could prove fruitful.

  16. A plant culture system for producing food and recycling materials with sweetpotato in space

    Kitaya, Yoshiaki; Yano, Sachiko; Hirai, Hiroaki


    The long term human life support in space is greatly dependent on the amounts of food, atmospheric O2 and clean water produced by plants. Therefore, the bio-regenerative life support system such as space farming with scheduling of crop production, obtaining high yields with a rapid turnover rate, converting atmospheric CO2 to O2 and purifying water should be established with employing suitable plant species and varieties and precisely controlling environmental variables around plants grown at a high density in a limited space. We are developing a sweetpotato culture system for producing tuberous roots as a high-calorie food and fresh edible leaves and stems as a nutritive functional vegetable food in space. In this study, we investigated the ability of food production, CO2 to O2 conversion through photosynthesis, and clean water production through transpiration in the sweetpotato production system. The biomass of edible parts in the whole plant was almost 100%. The proportion of the top (leaves and stems) and tuberous roots was strongly affected by environmental variables even when the total biomass production was mostly the same. The production of biomass and clean water was controllable especially by light, atmospheric CO2 and moisture and gas regimes in the root zone. It was confirmed that sweetpotato can be utilized for the vegetable crop as well as the root crop allowing a little waste and is a promising functional crop for supporting long-duration human activity in space.

  17. The Same but Different: Making Meaning from Modified Texts with Cross-Cultural Themes

    Leung, Cynthia B.; Bennett, Susan V.; Gunn, AnnMarie Alberton


    Reader response theory provides the framework for the present study that explored literary elements and cultural responses of fifth-grade students to two modified versions of a cross-cultural text, "Homesick: My Own Story" by Jean Fritz. One group of students read the first chapter of the book and another group read a modified basal…

  18. Post-empiricism and psychiatry: meaning and methodology in cross-cultural research.

    Bracken, P J


    Methodological disputes in psychiatry have usually been framed in terms of a positivist-interpretivist paradigm conflict. While traditional cross-cultural psychiatry does indeed endorse a positivist medical model the author argues that a post-empiricist philosophy of science is best able to 'ground' the 'new' cross-cultural approach.

  19. In vivo ectopic bone formation by devitalized mineralized stem cell carriers produced under mineralizing culture condition.

    Chai, Yoke Chin; Geris, Liesbet; Bolander, Johanna; Pyka, Grzegorz; Van Bael, Simon; Luyten, Frank P; Schrooten, Jan


    Functionalization of tissue engineering scaffolds with in vitro-generated bone-like extracellular matrix (ECM) represents an effective biomimetic approach to promote osteogenic differentiation of stem cells in vitro. However, the bone-forming capacity of these constructs (seeded with or without cells) is so far not apparent. In this study, we aimed at developing a mineralizing culture condition to biofunctionalize three-dimensional (3D) porous scaffolds with highly mineralized ECM in order to produce devitalized, osteoinductive mineralized carriers for human periosteal-derived progenitors (hPDCs). For this, three medium formulations [i.e., growth medium only (BM1), with ascorbic acid (BM2), and with ascorbic acid and dexamethasone (BM3)] supplemented with calcium (Ca(2+)) and phosphate (PO4 (3-)) ions simultaneously as mineralizing source were investigated. The results showed that, besides the significant impacts on enhancing cell proliferation (the highest in BM3 condition), the formulated mineralizing media differentially regulated the osteochondro-related gene markers in a medium-dependent manner (e.g., significant upregulation of BMP2, bone sialoprotein, osteocalcin, and Wnt5a in BM2 condition). This has resulted in distinguished cell populations that were identifiable by specific gene signatures as demonstrated by the principle component analysis. Through devitalization, mineralized carriers with apatite crystal structures unique to each medium condition (by X-ray diffraction and SEM analysis) were obtained. Quantitatively, BM3 condition produced carriers with the highest mineral and collagen contents as well as human-specific VEGF proteins, followed by BM2 and BM1 conditions. Encouragingly, all mineralized carriers (after reseeded with hPDCs) induced bone formation after 8 weeks of subcutaneous implantation in nude mice models, with BM2-carriers inducing the highest bone volume, and the lowest in the BM3 condition (as quantitated by nano-computed tomography

  20. Silence, nostalgia, violence, poverty … : what does 'culture' mean for South African sexuality educators?

    Depalma, Renée; Francis, Dennis


    In in-depth interviews with 25 Life Orientation teachers in South Africa, we found that teachers spontaneously drew upon notions of culture to explain and justify people's sexual beliefs and behaviours and their own role as educators. Drawing upon a Bakhtinian understanding of discourse, we apply critical semantic analysis to explore how culture is deployed as a discursive strategy. Teachers draw upon particular understandings of culture available to them in their social contexts. Furthermore, the substitution of the word 'culture' for a series of other phenomena (silence, violence and poverty) affords these phenomena a certain authority that they would otherwise not wield. We argue, first, that systems teacher education and training needs to (re)define culture as dynamic, interactive and responding to, but not determined by, socio-historical realities. Beyond this, teachers need to learn how to critically engage with cultural practices and perceptions and to be provided with some basic tools to do so, including more sophisticated understandings of cultural and training in dialogic methodologies. Teaching sexuality education in multicultural societies such as South Africa will require meaningful engagement in intercultural dialogues that may need to include voices that have traditionally been excluded from school spaces.

  1. National character does not reflect mean personality trait levels in 49 cultures

    Terracciano, A; Abdel-Khalek, A M; Adám, N


    Most people hold beliefs about personality characteristics typical of members of their own and others' cultures. These perceptions of national character may be generalizations from personal experience, stereotypes with a "kernel of truth," or inaccurate stereotypes. We obtained national character...... ratings of 3989 people from 49 cultures and compared them with the average personality scores of culture members assessed by observer ratings and self-reports. National character ratings were reliable but did not converge with assessed traits. Perceptions of national character thus appear to be unfounded...

  2. On Cultural Words, Cultural Meaning Words and Its Translation Method%浅议文化词和文化含义词及其翻译方法



    语言和文化的关系是辩证统一的,翻译过程中蕴含在语言中的文化因素给译者的工作带来了挑战。文章介绍了文化词和文化含义词的各自定义及区别,并对二者在英汉互译中的处理提出了几种方法。%The relationship between language and culture is a dialectical unity, and the cultural factors contained in translation process bring challenges to translator's work. This paper introduces the definitions and differences between cultural words and cultural meaning words, proposes several method in translation.

  3. Simple identification of Trichophyton tonsurans by chlamydospore-like structures produced in culture media.

    Mochizuki, Takashi; Anzawa, Kazushi; Sakata, Yuichi; Fujihiro, Machiko


    Trichophyton tonsurans is known to be the causative agent of a worldwide epidemic of dermatophytoses among contact sports practitioners, and is spreading among the general population of Japan. Prompt and simple identification of T. tonsurans in diagnostic laboratories is crucial to control infection. The present study evaluated the availability of observation of chlamydospore-like structures grown in culture media as a characteristic for identification of T. tonsurans. Twenty-five strains of T. tonsurans and five strains each of Trichophyton verrucosum, Trichophyton rubrum and Trichophyton mentagrophytes were inoculated on Mycosel agar plates and inoculated Petri dishes were observed by light microscopy from the reverse side. Twenty-three of 25 T. tonsurans strains showed chlamydospore-like structures within 5 days, and all strains at day 8. The numbers of chlamydospore-like structures were very abundant in most strains. The majority of strains of other species showed no chlamydospore-like structures, or very few when present. Positive for chlamydospore-like structures among 15 strains other than T. tonsurans was one strain at day 5 and six strains at day 8. As for the identification of T. tonsurans, presence of chlamydospore-like structures showed 92.0% sensitivity (23/25) and 93.3% specificity (14/15) at day 5, and 100% sensitivity (25/25) and 60.0% specificity (9/15) at day 8. Electron microscopic findings suggest chlamydospore-like structures are not true chlamydospores but are produced by inflation of actively growing hyphae by developing vacuoles in cells. In conclusion, observation of development of chlamydospore-like structures in culture media is the simplest method for identification of T. tonsurans.

  4. The uniqueness of cultures, universality and normativity – with special reference to the normative meaning of differentiation

    D.F.M. Strauss


    Full Text Available The inevitable presence of similarities between different cultures sets a limit to the (irrationalist claim that the uniqueness of every culture precludes the comparability of cultures. Investigating the background of this untenable view opens up the way to arrive at a reassessment of the role of continuity and universality. This provides the basis of an account of the normative meaning of biotical analogies within the various normative aspects of reality. The characterization of undifferentiated societies in a contrasting way highlights the normative meaning of societal differentiation. Examples of an excessive expansion of the power of particular societal spheres, such as the domain of the church or the scope of science (during the Middle Ages and the modern development of society, demonstrate that the course of factual events frequently does not observe the normative (sphere-sovereign structural boundaries for differentiated societal collectivities.




    Full Text Available The problems encountered in today's urban spaces by Tahtacis, who are one of the major building blocks of Anatolian culture, constitute a significant field of discussion. In urban spaces, the historical images of Tahtaci groups that are integrated with rural spaces seem quite far from the descriptions of 'agaceri', 'nomad' or 'semi-nomad'. The new practices of life emerged parallel with the changes which social organisation models of Tahtacis have gone through in terms of time and space require an evaluation of Tahtaci identity and culture within the frame of different forms. This article which is beyond the previous studies concerning Tahtaci groups about ethnicity and religious practices analyses the current social condition of Tahtacis who live in Isparta Turan district in terms of perception of space. Beside the problems that Tahtaci culture and identity have in the transmission of oral culture, the social profiles that appear with the conditions of being entrapped and unavoidably scattered in urban space are the major points of examination. In this study, the new constructions and forms of space, culture and identity relations are scrutinised within a sociological perspective.

  6. Optimization of Chlorella vulgaris and bioflocculant-producing bacteria co-culture: enhancing microalgae harvesting and lipid content.

    Wang, Y; Yang, Y; Ma, F; Xuan, L; Xu, Y; Huo, H; Zhou, D; Dong, S


    Microalgae are a sustainable bioresource, and the biofuel they produce is widely considered to be an alternative to limited natural fuel resources. However, microalgae harvesting is a bottleneck in the development of technology. Axenic Chlorella vulgaris microalgae exhibit poor harvesting, as expressed by a flocculation efficiency of 0·2%. This work optimized the co-culture conditions of C. vulgaris and bioflocculant-producing bacteria in synthetic wastewater using response surface methodology (RSM), thus aiming to enhance C. vulgaris harvesting and lipid content. Three significant process variables- inoculation ratio of bacteria and microalgae, initial glucose concentration, and co-culture time- were proposed in the RSM model. F-values (3·98/8·46) and R(2) values (0·7817/0·8711) both indicated a reasonable prediction by the RSM model. The results showed that C. vulgaris harvesting efficiency reached 45·0-50·0%, and the lipid content was over 21·0% when co-cultured with bioflocculant-producing bacteria under the optimized culture conditions of inoculation ratio of bacteria and microalgae of 0·20-0·25, initial glucose concentration of microalgae harvesting and cost-effective microalgal bioproducts, and confirmed the promising prospect of introducing bioflocculant-producing bacteria into microalgae bioenergy production. This work optimized the co-culture conditions of microalgae (C. vulgaris) and bioflocculant-producing bacteria (F2, Rhizobium radiobacter) in synthetic wastewater using response surface methodology, aiming to enhance C. vulgaris harvesting and lipid produced content. Bioflocculant-producing microbes are environmentally friendly functional materials. They avoid the negative effects of traditional chemical flocculants. This work provided new insights into microalgae harvesting and cost-effective production of microalgal bioproducts, and confirmed the promising prospect of introducing bioflocculant-producing bacteria into microalgae

  7. Folk national culture as a means of forming norms of communication in childhood

    Chernushevich V. A.


    Full Text Available The best carriers of playing culture are children, who possess and enjoy it. Destroyed social kids’ structures, territorial kids’ associations (family, yard, village, street communities of children interrupted generally the process of culture transmission, reproduction and passing of communication tradition. And there is a need in social-state “revivification” (recovering folk games list and its’ players, enough for folk games reproduction process. Folk game includes particular properties of relations on the levels of physical and emotional, vocal interaction, imagery-symbolic filling, special features of clothes (all aspects of communication that constitute features of national culture of the nation and make from the nation the community of people very special and different from other communities and nations. Studying of correctional possibilities of folk games within the frames of playing agendas showed that their psychological and emotional resources provide the conditions for adoption by children the norms of communication.

  8. Design, Fabrication, and Operation of Innovative Microalgae Culture Experiments for the Purpose of Producing Fuels: Final Report, Phase I


    A conceptual design was developed for a 1000-acre (water surface) algae culture facility for the production of fuels. The system is modeled after the shallow raceway system with mixing foils that is now being operated at the University of Hawaii. A computer economic model was created to calculate the discounted breakeven price of algae or fuels produced by the culture facility. A sensitivity analysis was done to estimate the impact of changes in important biological, engineering, and financial parameters on product price.

  9. The meaning of pain: a cultural formulation of a Syrian woman in Sweden.

    Bäärnhielm, Sofie


    Ethnography provides a method for psychiatric assessment to obtain an insight into the patient's culture, context, and life situation. The Outline for a Cultural Formulation (CF) is an ethnography-based, idiographic formulation intended to complement the multiaxial assessment in DSM-IV. Its contribution to routine clinical praxis will be discussed with reference to a case of a Syrian-born woman in Sweden. Using the CF in the clinical diagnostic process shifted understanding of the patient's suffering from a mainly somatic frame of reference to an emphasis on emotional and social aspects. The usefulness of ethnography in clinical psychiatric diagnostic practice is discussed.

  10. The Cultural Meaning of Cardiac Illness and Self-Care Among Lebanese Patients With Coronary Artery Disease.

    Dumit, Nuhad Yazbik; Magilvy, Joan Kathy; Afifi, Rima


    Cardiac disease is the leading cause of death in Lebanon, accounting for 22% to 26% of total deaths in the country. A thorough understanding of perceptions of cardiac illness and related self-care management is critical to the development of secondary prevention programs that are specific to the Lebanese culture. To explore the cultural perceptions of cardiac illness and the associated meaning of self-care among Lebanese patients. Using a qualitative descriptive method, semistructured interviews were conducted with a purposive sample of 15 Lebanese cardiac patients recruited from a medical center in Beirut, Lebanon. The qualitative descriptive analysis yielded one overarching and two other themes describing perceptions of cardiac illness and self-care within the Lebanese cultural context. The overarching cultural theme was, "Lebanese cardiac patients were unfamiliar with the term concept and meaning of self-care." Lebanese cardiac patients thanked God and accepted their fate (Theme I). The participants considered their cardiac incident a life or death warning (Theme II). Health care providers need to consider patients' cultural perception of illness while planning and evaluating cardiac self-care programs. © The Author(s) 2015.

  11. Rapid Identification of Shiga Toxin-Producing Escherichia coli O Serogroups from Fresh Produce and Raw Milk Enrichment Cultures by Luminex Bead-Based Suspension Array.

    Kase, Julie A; Maounounen-Laasri, Anna; Lin, Andrew


    The U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) Chapter 4a describes a Luminex microbead-based suspension array used to screen colonies for 11 clinically relevant Shiga toxin-producing Escherichia coli (STEC) serogroups: O26, O45, O91, O103, O104, O111, O113, O121, O128, O145, and O157. We evaluated the usefulness of this method to identify STEC-positive enrichment samples before agar plating. Twelve E. coli strains were added to three types of fresh produce (bagged baby spinach, alfalfa sprouts, and cilantro) at levels near the detection limit of the test. A subset of these strains (six O serogroups) was similarly evaluated in raw milk. For comparison, portions of each of the 168 enrichment cultures were analyzed for serogroup by a real-time PCR assay and a Bio-Plex 200 assay with the bead-based suspensions. No false-positive results were obtained. Of the 112 samples with a reported cycle threshold (CT) value, 101 undiluted, diluted, or extracted enrichment cultures also produced ratios above 5.0 in the Bio-Plex assay. When PCR CT values approached or were greater than 35, Bio-Plex detection became less reliable. Using undiluted or extracted enrichment cultures resulted in a significantly larger number of positive results. With the same enrichment material prepared for real-time PCR analysis as described in the BAM Chapter 4a, the STEC microbead-based suspension array can accurately screen food enrichment cultures.

  12. Librarians and Party Girls: Cultural Studies and the Meaning of the Librarian.

    Radford, Marie L.; Radford, Gary P.


    Responds to Wayne Wiegand's article that criticizes library and information science by suggesting a cultural studies approach to the field. Applies the work of Stuart Hall to a media stereotype of the female librarian based on the film "Party Girl" to allow new interpretations of media images of the profession. (Author/LRW)

  13. Ways and Means of Adapting Culture and Structure: Case Studies. Support Document 1

    Clayton, Berwyn; Fisher, Thea; Harris, Roger; Bateman, Andrea; Brown, Mike


    The resource in this support document is a set of small case studies, offering insights into how a range of organisations have gone about adapting their organisational structure and/or culture to enhance their capability. Key elements of each case are presented with a particular emphasis on: (1) the principles that have underpinned each approach…

  14. Conflicts of Identity, Conservation, and Cultural Heritage Meaning Management: Reading through ICOMOS Charters

    Kamel Ahmed, Ehab


    This book "Constructing Intangible Heritage" gathers a set of articles \\ud organised in four chapters, under the thematic of intangible heritage:\\ud - Towards the immateriality of heritage;\\ud - Conceptualizing intangible heritage;\\ud - Intangible heritage and cultural manifestations;\\ud - The museology of intangible heritage.

  15. Storytelling: A Means to Revitalize a Disappearing Language and Culture in Northeast Thailand (Isan)

    Tossa, Wajuppa


    Throughout much of northeast Thailand (Isan), Lao is the dominant local language. Today, however, central and official Thai is rapidly becoming the dominant language throughout Isan. It is feared that Thailand may become monocultured and its citizens may lose their diversity in languages and culture. In this article, the author describes a…

  16. Analysis of microbial community variation during the mixed culture fermentation of agricultural peel wastes to produce lactic acid.

    Liang, Shaobo; Gliniewicz, Karol; Gerritsen, Alida T; McDonald, Armando G


    Mixed cultures fermentation can be used to convert organic wastes into various chemicals and fuels. This study examined the fermentation performance of four batch reactors fed with different agricultural (orange, banana, and potato (mechanical and steam)) peel wastes using mixed cultures, and monitored the interval variation of reactor microbial communities with 16S rRNA genes using Illumina sequencing. All four reactors produced similar chemical profile with lactic acid (LA) as dominant compound. Acetic acid and ethanol were also observed with small fractions. The Illumina sequencing results revealed the diversity of microbial community decreased during fermentation and a community of largely lactic acid producing bacteria dominated by species of Lactobacillus developed.


    Kononova Inna Vladimirovna


    Full Text Available The article considers some modern approaches to the analysis of the structure of a cultural concept and introduces a new method that is an efficient addition to current trends on the concept structure investigation accepted in modern linguistics. The structure of a cultural concept is viewed as including imagery, associative, notional, evaluative, etymological and historical components. The national peculiarity of a cultural concept is reflected mainly in its evaluative and associative components and is largely determined by its etymological component. The associative component of a cultural concept is formed by the dominant associative relations (thematic, paradigmatic, syntagmatic of its language representatives. The notional component has some intersection zones with the associative one as it can be drawn from the dominant associative links. However, the generic features of a concept constitute the basic part of the notional component. The imagery component of a concept may include a visually-perceptible representation (a perceptual image or a combination of conceptual metaphors deduced by a native speaker from the collocations of the word(s, representing the concept in the language. The suggested method can be used in analyzing the diachronic changes of the national concept sphere. Theoretical statements are exemplified with the structural analysis of the British ethical concept SELF-POSSESSION. The language data observation helps to discover the interrelations of the concept in question with some other dominant concepts of the ethical concept sphere represented by British English. The author emphasizes cultural importance of the concept within the bounds of the British national mentality. Representation of the analyzed concept in the phraseological fund of the language is in the focus of the author's attention.

  18. Low molecular weight peptides derived from sarcoplasmic proteins produced by an autochthonous starter culture in a beaker sausage model

    Constanza M. López


    Significance: The selection of a specific autochthonous starter culture guarantees the hygiene and typicity of fermented sausages. The identification of new peptides as well as new target proteins by means of peptidomics represents a significant step toward the elucidation of the role of microorganisms in meat proteolysis. Moreover, these peptides may be further used as biomarkers capable to certify the use of the applied autochthonous starter culture described here.

  19. Rehabilitation of adolescent patients with cerebral palsy by means of physical culture.

    Vindiuk P.A.


    Full Text Available It is researched the effect of exercise in the promptness with mobile games on the power supply of the body of adolescents with cerebral palsy. The study involved 16 adolescent schoolchildren with spastic forms of cerebral palsy of varying severity. In order to determine their level of functional training has been used S.A. Dushanina's multifactor express-diagnostics. It is shown indications of the possibility of multi-factor for the rapid diagnosis of this group. It is established that the lessons of physical culture, which includes a specially organized outdoor games, elements of switch room training and training on cycling performance of functional contribute to the growth of training and have a positive effect on the body of adolescents with cerebral palsy as a whole. Aim of work - to estimate influence of employments a physical culture on energy-supply of organism of teenagers with cerebral palsy.

  20. Cultural Considerations: Pharmacological and Nonpharmacological Means for Improving Blood Pressure Control among Hispanic Patients

    Neela K. Patel


    Full Text Available Cardiovascular disease is a leading cause of morbidity and mortality in the United States, and its prevention and treatment remain a priority for the medical community. Ethnic variations account for some differences in the prevalence of hypertension and blood pressure (BP control rates among Hispanics, indicating the need for culturally appropriate management models. Aggressive treatment strategies are key to achieving optimal BP control in high-risk Hispanic patients. Hypertension in this ethnic group continues to be a major health concern. Of note, when provided access to comprehensive care, Hispanics demonstrate similar response rates to treatment as the majority of non-Hispanic whites. This highlights the importance of effective, culturally responsive hypertension management among high-risk Hispanic patients for achieving observable, positive health outcomes.

  1. For the Benefit of Others: Generativity and Meaning in Life in the Elderly in Four Cultures

    J. Hofer; Holger, B.; Au, A.; Poláčková Šolcová, I. (Iva); Tavel, P.; Wong, T. T.


    Generativity has been argued to be an important indicator of successful aging. Indeed, generative concern has been found to be associated with meaning in life. In the present study, this relationship is argued to be partly explained through generative goals. Moreover, the path between generative goals and meaning in life is hypothesized to be conditional on belief in the species (assessed through Machiavellianism as a proxy variable). This moderated mediation model is tested with data from 4 ...

  2. Formation of culture motor activity of pupils of 5-6 classes by means of basic gymnastics

    Deyneko A.H.


    Full Text Available Purpose : methodological conditions justify the use of basic gymnastics in physical education of pupils of secondary schools. The experimental test method creating a culture of motor activity by means of basic gymnastics in the system of physical education students grades 5-9. Material : experiment were selected 109 students. Results : The method comprises three stages: basic, special and profound. The developed method should be applied in the preparatory part of the lesson (15-20 minutes - invariant component of the curriculum. Found that means basic gymnastics culture is the basis for motor activity. Their conscious and correct implementation is a prerequisite to the motivational desire to exercise. Conclusions : It is proposed to evaluate the physical condition of students with criteria: compliance burden of a preparatory lesson preparedness and age of students; lack of fatigue during exercise basic gymnastics; the impact of stress on technique exercises.

  3. A Single Dynamic Metabolic Model Can Describe mAb Producing CHO Cell Batch and Fed-Batch Cultures on Different Culture Media.

    Robitaille, Julien; Chen, Jingkui; Jolicoeur, Mario


    CHO cell culture high productivity relies on optimized culture medium management under fed-batch or perfused chemostat strategies enabling high cell densities. In this work, a dynamic metabolic model for CHO cells was further developed, calibrated and challenged using datasets obtained under four different culture conditions, including two batch and two fed-batch cultures comparing two different culture media. The recombinant CHO-DXB11 cell line producing the EG2-hFc monoclonal antibody was studied. Quantification of extracellular substrates and metabolites concentration, viable cell density, monoclonal antibody concentration and intracellular concentration of metabolite intermediates of glycolysis, pentose-phosphate and TCA cycle, as well as of energetic nucleotides, were obtained for model calibration. Results suggest that a single model structure with a single set of kinetic parameter values is efficient at simulating viable cell behavior in all cases under study, estimating the time course of measured and non-measured intracellular and extracellular metabolites. Model simulations also allowed performing dynamic metabolic flux analysis, showing that the culture media and the fed-batch strategies tested had little impact on flux distribution. This work thus paves the way to an in silico platform allowing to assess the performance of different culture media and fed-batch strategies.

  4. Enterococcus faecium RZS C5, an interesting bacteriocin producer to be used as a co-culture in food fermentation.

    Leroy, F; Foulquié Moreno, M R; De Vuyst, L


    Enterocins, bacteriocins produced by enterococci, are gaining interest because of their industrial potential. Due to its bacteriocin production, Enterococcus faecium RZS C5, a natural cheese isolate, has a strong activity towards Listeria monocytogenes. For this reason, the strain may be applicable as a bacteriocin-producing co-culture in food fermentation in order to reduce the risk on Listeria outgrowth. The strain displays remarkable bacteriocin production kinetics. Whereas most lactic acid bacteria produce bacteriocin in a growth-associated way until the beginning of the stationary phase, bacteriocin production by E. faecium RZS C5 in MRS broth at controlled pH values below 7.5 is characterised by a boost of bacteriocin activity levels in the very early growth phase. In addition, bacteriocin production kinetics are closely linked to the environmental and cultural conditions. However, no straightforward statement about the effect of environmental stress on bacteriocin production can be made since the effect is dependent on the type of stress applied. Kinetic experiments in milk and on pilot scale, applying Cheddar cheese-making conditions, have indicated that the strain may be effective as a bacteriocin-producing co-culture. Further research is needed to evaluate the use of E. faecium RZS C5 as a co-culture for the production of fermented sausage.

  5. Fermentanomics informed amino acid supplementation of an antibody producing mammalian cell culture.

    Read, Erik K; Bradley, Scott A; Smitka, Tim A; Agarabi, Cyrus D; Lute, Scott C; Brorson, Kurt A


    Fermentanomics, or a global understanding of a culture state on the molecular level empowered by advanced techniques like NMR, was employed to show that a model hybridoma culture supplied with glutamine and glucose depletes aspartate, cysteine, methionine, tryptophan, and tyrosine during antibody production. Supplementation with these amino acids prevents depletion and improves culture performance. Furthermore, no significant changes were observed in the distribution of glycans attached to the IgG3 in cultures supplemented with specific amino acids, arguing that this strategy can be implemented without fear of impact on important product quality attributes. In summary, a targeted strategy of quantifying media components and designing a supplementation strategy can improve bioprocess cell cultures when enpowered by fermentanomics tools.

  6. Jazz and its Social Meanings in Iran: From Cultural Colonialism to the Universal

    Nooshin, L.


    July 10, 2000. A hot summer evening. An excited crowd, mainly young people including many fashionable young women, heavily made up and wearing the latest “Tehran Chic,” wait outside the Ebn- e Sina Cultural Centre in Shahrak- e Qarb, a well- to- do neighborhood in west Tehran. The “jazz” concert by the band Imaj is scheduled to start at 7:00 p.m., but at 7:15 p.m. the doors are still closed. Eventually, the doors are opened and the audience streams in to the small concert hall. The sense of a...

  7. "Buying Local" Means "Selling Local" - Using a Transportation Alliance of environmental and Food Horticulture Producers in Georgia

    Stegelin, Forrest E.; Houston, Jack E.; Compte, Javier Mantilla; Thomas, Paul A.; Chappell, Matthew R.


    The costs of transporting agricultural products to market and of the acquisition of production inputs are of concern to environmental and food horticulture crops producers. This study is to determine if transportation alliances would reduce shipping costs, increase distribution efficiencies and reduce carbon dioxide emissions among floriculture, ornamental plants, and fruit and vegetable producers in Georgia. Using data from surveys and the ArcLogistics 9.3 GIS software, routing systems were ...

  8. Reconsidering Meaning Making and Its Analytical Implications for Cultural Studies of Science Education

    Arnseth, Hans Christian; Krange, Ingeborg


    The discussion in this forum aims to push some of the ideas further and thus clarify some of the concepts used in the feature article. This includes a debate of the concepts of meaning making, procedural and conceptual learning experiences, the distinction between realism and constructivism in our research practice, and finally how to foster…

  9. Reconsidering meaning making and its analytical implications for cultural studies of science education

    Arnseth, Hans Christian; Krange, Ingeborg


    The discussion in this forum aims to push some of the ideas further and thus clarify some of the concepts used in the feature article. This includes a debate of the concepts of meaning making, procedural and conceptual learning experiences, the distinction between realism and constructivism in our research practice, and finally how to foster productive disciplinary engagement.

  10. Heterotrophic high cell-density fed-batch cultures of the phycocyanin-producing red alga Galdieria sulphuraria.

    Schmidt, Rikke Ankerstjerne; Wiebe, Marilyn G; Eriksen, Niels Thomas


    Growth and phycocyanin production in batch and fed-batch cultures of the microalga Galdieria sulphuraria 074G, which was grown heterotrophically in darkness on glucose, fructose, sucrose, and sugar beet molasses, was investigated. In batch cultures, specific growth rates and yields of biomass dry weight on the pure sugars were 1.08-1.15 day-1 and 0.48-0.50 g g-1, respectively. They were slightly higher when molasses was the carbon source. Cellular phycocyanin contents during the exponential growth phase were 3-4 mg g-1 in dry weight. G. sulphuraria was able to tolerate concentrations of glucose and fructose of up to 166 g L-1 (0.9 M) and an ammonium sulfate concentration of 22 g L-1 (0.17 M) without negative effects on the specific growth rate. When the total concentration of dissolved substances in the growth medium exceeded 1-2 M, growth was completely inhibited. In carbon-limited fed-batch cultures, biomass dry weight concentrations of 80-120 g L-1 were obtained while phycocyanin accumulated to concentrations between 250 and 400 mg L-1. These results demonstrate that G. sulphuraria is well suited for growth in heterotrophic cultures at very high cell densities, and that such cultures produce significant amounts of phycocyanin. Furthermore, the productivity of phycocyanin in the heterotrophic fed-batch cultures of G. sulphuraria was higher than is attained in outdoor cultures of Spirulina platensis, where phycocyanin is presently obtained.


    T. Ahmed and R. Kanwal


    Full Text Available Lactic acid bacteria (LAB were isolated from camel milk by culturing the milk on specific media and pure culture was obtained by sub-culturing. Purification of culture was confirmed by Gram’s staining and identified by different biochemical tests. Camel milk contained lactic acid producing bacteria like Streptococci such as S. cremoris and S. lactis and Lactobacilli such as L. acidophilus. L. acidophilus grew more rapidly in camel milk than others as its growth was supported by camel milk. Ability of each strain was tested to convert lactose of milk into lactic acid. It was observed that 66% lactose was converted by S. lactis 20, whereas S. cremoris 22 and L. acidophilus 23 converted 56 and 74% lactose into lactic acid, respectively. Effect of freeze-drying was also recorded and the results showed that in all cases there was a slight decrease in the cell count before and after the freeze-drying. The decrease was approximately 0.47, 0.078 and 0.86% for S. lactis 20, S. cremoris 22 and L. acidophilus 23, respectively. Starter culture was prepared from strains isolated from camel milk. Camel and buffalo milk cheese was prepared by using starter culture. The strains isolated from camel milk were best for acid production and coagulated the milk in less time. It is concluded that cheese can be prepared successfully from camel milk and better results can be obtained by coagulating milk with starter culture.

  12. Making sense of HIV in southeastern Nigeria: fictional narratives, cultural meanings, and methodologies in medical anthropology.

    Winskell, Kate; Brown, Peter J; Patterson, Amy E; Burkot, Camilla; Mbakwem, Benjamin C


    Fictional narratives have rarely been used in medical anthropological research. This article illustrates the value of such narratives by examining how young people in southeastern Nigeria navigate the cultural resources available to them to make sense of HIV in their creative writing. Using thematic data analysis and narrative-based methodologies, it analyzes a sample (N = 120) from 1,849 narratives submitted by Nigerian youth to the 2005 Scenarios from Africa scriptwriting contest on the theme of HIV. The narratives are characterized by five salient themes: tragedy arising from the incompatibility of sex outside marriage and kinship obligations; female vulnerability and blame; peer pressure and moral ambivalence; conservative Christian sexual morality; and the social and family consequences of HIV. We consider the strengths and limitations of this narrative approach from a theoretical perspective and by juxtaposing our findings with those generated by Daniel Jordan Smith using standard ethnographic research methods with a similar Igbo youth population.

  13. Meaning of Brands for Consumers in their Social Interactions: Appeal of Symbolic Expressions of Cultural Identity

    André Luiz Maranhão de Souza Leão


    Full Text Available Much has been discussed about how the consumer has become the core trigger for changes within contemporary society. In this regard, it is considered that identities are formed by consumer choices. The identities are not expressed by the products chosen, per se, but by what the products represent. Thus, we deduce that the purchase of brands is a mainstay of the construction of cultural identities. With this in mind, this study aimed to understand how consumers construct their identities by using brands as symbolic resources in their social interactions in everyday life. To this end, an ethnography of communication was conducted through participant observation of everyday interactions of people from different social groups. The results indicated eleven types of identity, divided into three categories: communal, social, and personal. In the end, we analyzed the possible contributions of the study for both academia and for brand management.

  14. Afterbirths in the afterlife: cultural meaning of placental disposal in a Hmong American community.

    Helsel, Deborah G; Mochel, Marilyn


    Interviews were conducted with 94 Hmong Americans in California's Central Valley to explore attitudes regarding placental disposition and the cultural values that affect those attitudes. Research indicated a persistence of the traditional belief that placentas should be buried at home. The placenta is perceived to be essential for travel by the soul of the deceased into the spirit world to rejoin ancestors. Older respondents (older than age 35) and those who self-identified as animists were most likely to believe in the importance of home placental burial. Comments by respondents indicated some reluctance on the part of Hmong patients to ask health care providers for permission to take placentas home. Incorporating non-Western patients' traditional health care practices into Western health care delivery may be facilitated by an awareness of the reluctance of some patients to verbalize their wishes.

  15. In vitro culture of hybridoma cells in agarose beads producing antibody secretion for two weeks.

    Cadic, C; Dupuy, B; Pianet, I; Merle, M; Margerin, C; Bezian, J H


    A new process for embedding cells in agarose is described. Beads were obtained by extruding an ultralow gelling temperature agarose solution in a capillary containing a hydrophobic medium flowthrough. The toxicity of the procedure has been evaluated by monitoring the energy status of agarose-embedded C(6) glioma cells with (31)P nuclear magnetic resonance (NMR). Suspension and microbead cultures of hybridoma cell line were compared. In suspension culture the number of cells and the antibody concentrations increased for 5 days before the stationary phase began, when the cultures were stopped. In agarose bead cultures, the gel provided an enormous support surface area (50 m(2)/ mL of gel). It was possible to seed 20-fold more cells. The gel pressure modified the proliferative process and antibody pattern secretion. In particular, the antibodies could be harvested for two weeks.

  16. [Method of producing fusarin C in perlite-liquid culture medium].

    Li, G; Li, M; Ma, J


    For researching the biosynthesis labelled Fusarin C(Fc) by Fuscarium moniliforme, a more quick and convenient method of Fusarin C production and purification were established, and a good liquid culture medium consisted of different kinds organic matters (hydroxy proline, sucrose and glycerin), inorganic salts and perlite replaced corn grit medium. The perlite-liquid culture medium inoculated with the strain of F. moniliforme yields 936mg Fc/kg organic matter with in 14 days of incubation at 28 degrees C. As compared with the corn grit medium, the amount of Fc from perlite-liquid medium was more than that from corn grit medium (831mg Fc/kg corn grit). In all experiments both thin-layer chromatography and high-pressure liquid chromatography were used to confirm the presence of Fc. parameters which were important for the optimal biosynthesis of Fc included hydroxy proline and sucrose concentrations, incubated time/temperature and amount of perlite. The 40g of sucrose/L liquid culture was optimal concentration for Fusarin C production. Of three contained N-matter tested, hydroxy proline was the best sources of N-atom for Fusarin C. Under the absence of hydroxy proline, the Fc wasn't synthesized in perlite-liquid culture medium by F. moniliforme. A culture time/temperature study of Fc production was done, and the optimal Fc amounts was synthesized after incubation for 14 days at 28 degrees C on perlite-liquid culture medium.

  17. Impact of nisin producing culture and liposome-encapsulated nisin on ripening of Lactobacillus added-Cheddar cheese.

    Benech, R O; Kheadr, E E; Lacroix, C; Fliss, I


    This study aimed to evaluate the effects of incorporating liposome-encapsulated nisin Z, nisin Z producing Lactococcus lactis ssp. lactis biovar. diacetylactis UL719, or Lactobacillus casei-casei L2A adjunct culture into cheese milk on textural, physicochemical and sensory attributes during ripening of Cheddar cheese. For this purpose, cheeses were made using a selected nisin tolerant cheese starter culture. Proteolysis, free fatty acid production, rheological parameters and hydrophilic/hydrophobic peptides evolution were monitored over 6 mo ripening. Sensory quality of cheeses was evaluated after 6 mo. Incorporating the nisin-producing strain into cheese starter culture increased proteolysis and lipolysis but did not significantly affect cheese rheology. Liposome-encapsulated nisin did not appear to affect cheese proteolysis, rheology and sensory characteristics. The nisinogenic strain increased the formation of both hydrophilic and hydrophobic peptides present in the cheese water extract. Sensory assessment indicated that acidic and bitter tastes were enhanced in the nisinogenic strain-containing cheese compared to control cheese. Incorporating Lb. casei and the nisinogenic culture into cheese produced a debittering effect and improved cheese flavor quality. Cheeses with added Lb. casei and liposome-encapsulated nisin Z exhibited the highest flavor intensity and were ranked first for sensory characteristics.


    O. B. Sirotinina


    Full Text Available The article shows that in the 20th century the system of the Russian language and its usage began to develop not so much under the influence of fiction (belle-lettres as under the influence of mass media. In the 21st century due to the enhancing role of the Internet everyday speech became integrated into mass media on a large scale. So the Internet together with the radio and television, to a lesser extent with newspapers, has become the disseminator of language fashion. Lexicographic codification weakened its influence, and its drawbacks (vagueness of interpretation activated the tendency to reduce mental efforts in the usage of words, which resulted in the diffusion of meanings or the preference of the diffused word to its exact synonym.Everyday speech provides understanding, because it is related to the situation and immediate communication of the participants, who always a have a chance to ask an echo-question. Thas is not characteristic of mass media, yet they “impose” language fashion, which ultimately leads to language impoverishment, “newspeak” and hackneyed vocabulary.Diffusion of meanings is promoted by pop usage, time pressure, as well as the explanation of one word by means of another.The weakened tendency to use exact nominations and the atrengthened tendency to reduce mental efforts in word choice can be illustrated by the usage of “nadet’-odet’”, “dostatochno-dovol’no”, “shodnyj-pohozhij-shozhij” frequent in modern mass media (based on the data from Positive changes in the usage of these words (except “nadet’-odet’” that have been observed since the fall of 2012 give us hope to preserve synonymic wealth of Russian.

  19. Direct surfactin-gramicidin S antagonism supports detoxification in mixed producer cultures of Bacillus subtilis and Aneurinibacillus migulanus.

    Rautenbach, Marina; Eyéghé-Bickong, Hans André; Vlok, Nicolas Maré; Stander, Marietjie; de Beer, Abré


    Antibiotic production as a defence mechanism is a characteristic of a wide variety of organisms. In natural evolutionary adaptation, cellular events such as sporulation, biofilm formation and resistance to antibiotics enable some micro-organisms to survive environmental and antibiotic stress conditions. The two antimicrobial cyclic peptides in this study, gramicidin S (GS) from Aneurinibacillus migulanus and the lipopeptide surfactin (Srf) from Bacillus subtilis, have been shown to affect both membrane and intercellular components of target organisms. Many functions, other than that of antimicrobial activity, have been assigned to Srf. We present evidence that an additional function may exist for Srf, namely that of a detoxifying agent that protects its producer from the lytic activity of GS. We observed that Srf producers were more resistant to GS and could be co-cultured with the GS producer. Furthermore, exogenous Srf antagonized the activity of GS against both Srf-producing and non-producing bacterial strains. A molecular interaction between the anionic Srf and the cationic GS was observed with circular dichroism and electrospray MS. Our results indicate that the formation of an inactive complex between GS and Srf supports resistance towards GS, with the anionic Srf forming a chemical barrier to protect its producer. This direct detoxification combined with the induction of protective stress responses in B. subtilis by Srf confers resistance toward GS from A. migulanus and allows survival in mixed cultures.

  20. The meanings of 'culture' in health and social care: a case study of the combined Trust in Somerset.

    Peck, E; Towell, D; Gulliver, P


    On the 1st of April 1999 Somerset Health Authority and Somerset County Council established a Joint Commissioning Board (JCB) to commission mental health services. Simultaneously, the Somerset Partnerships Health and Social Care NHS Trust was set-up as a combined health and social care services provider; for the first time in England, the majority of social services mental health staff transferred their employment to this Trust. The JCB commissioned an evaluation of the impact of these innovations on users and carers, staff and senior officers and members in the organisations concerned. In the course of the research, one concept frequently recurred as a source of both optimism and concern: 'culture'. However, the meanings attributed to this term varied significantly between stakeholders. After summarising the methodology and the results to date of the evaluation, this paper briefly reviews the conceptions of culture in the literature on organisations, and introduces a taxonomy for discussing culture. That taxonomy is then deployed to explore the ways in which the notion of 'culture' is being applied to and within the combined Trust in Somerset, and the implications of those applications for local practice and national policy around partnership.

  1. Multicultural mathematics and its cultural meanings%多元文化数学及其文化意义

    唐恒钧; 张维忠


    Mathematics had been widely thought to be culture-free,however researches in multicultural mathe-matics indicated that mathematics was also culture-related. The concept of multicultural mathematics was ana-lyzed with respect to its theoretical basis,connotation and mechanism of development. Then the source and existing form of multicultural mathematics were discussed. At last,cultural meanings of multicultural mathe-matics were analyzed by concerning about cultural tradition,mathematics philosophy,history and mathematics education.%数学常被认为是文化自由的,多元文化数学则揭示了数学的文化相关性。从多元文化数学的立论基础、基本内涵及发展机制的角度分析了多元文化数学的涵义,并在此基础上讨论了多元文化数学的来源与存在形态。最后,从传统文化、数学哲学与数学史、数学教育等角度分析了多元文化数学的文化意义。

  2. Glycoprotein Ibα receptor instability is associated with loss of quality in platelets produced in culture.

    Robert, Amélie; Boyer, Lucie; Pineault, Nicolas


    The development of culture processes for hematopoietic progenitors could lead to the development of a complementary source of platelets for therapeutic purposes. However, functional characterization of culture-derived platelets remains limited, which raises some uncertainties about the quality of platelets produced in vitro. The aim of this study was to define the proportion of functional platelets produced in cord blood CD34+ cell cultures. Toward this, the morphological and functional properties of culture-derived platelet-like particles (PLPs) were critically compared to that of blood platelets. Flow cytometry combined with transmission electron microscopy analyses revealed that PLPs formed a more heterogeneous population of platelets at a different stage of maturation than blood platelets. The majority of PLPs harbored the fibrinogen receptor αIIbβ3, but a significant proportion failed to maintain glycoprotein (GP)Ibα surface expression, a component of the vWF receptor essential for platelet functions. Importantly, GPIbα extracellular expression correlated closely with platelet function, as the GPIIb+ GPIbα+ PLP subfraction responded normally to agonist stimulation as evidenced by α-granule release, adhesion, spreading, and aggregation. In contrast, the GPIIb+ GPIbα⁻ subfraction was unresponsive in most functional assays and appeared to be metabolically inactive. The present study confirms that functional platelets can be generated in cord blood CD34+ cell cultures, though these are highly susceptible to ectodomain shedding of receptors associated with loss of function. Optimization of culture conditions to prevent these deleterious effects and to homogenize PLPs is necessary to improve the quality and yields of culture-derived platelets before they can be recognized as a suitable complementary source for therapeutic purposes.

  3. Meaning of relative gene expression in multilayered cultures of epidermal keratinocytes.

    Malaisse, Jérémy; Hermant, Maryse; Hayez, Aurélie; Poumay, Yves; Lambert de Rouvroit, Catherine


    Reconstructed human epidermis (RHE) has become an in vitro model of choice for studying cell and tissue functions. Analysis of gene expression over the course of reconstruction must take into account the heterogeneous differentiation states of keratinocytes reconstituting the typical epidermal layers. In monolayer cultures, relative mRNA expression levels of differentiation markers are usually expressed as a ratio versus a classical reference gene (also named house-keeping gene) tested to be expressed equally in certain experimental conditions. Applied to complex tissues in which the cell number increases over time together with differentiation, calculation of relative gene expression does not take enough into account a crucial phenomenon: epidermal morphogenesis results in progressive restriction of differentiation markers, such as involucrin, to a specific layer, or in the delayed onset of mRNA expression of filaggrin or TMEM45A for instance following stratification. Our study illustrates that comparing the relative expression level of mRNAs to that of a basal layer-specific gene (e.g. ITGA6) better illustrates the contribution of specific differentiation markers to the process of epidermal morphogenesis.

  4. Identification of a New Marine Bacterial Strain SD8 and Optimization of Its Culture Conditions for Producing Alkaline Protease.

    Cui, Hongxia; Yang, Muyang; Wang, Liping; Xian, Cory J


    While much attention has been given to marine microorganisms for production of enzymes, which in general are relatively more stable and active compared to those from plants and animals, studies on alkaline protease production from marine microorganisms have been very limited. In the present study, the alkaline protease producing marine bacterial strain SD8 isolated from sea muds in the Geziwo Qinhuangdao sea area of China was characterized and its optimal culture conditions were investigated. Strain SD8 was initially classified to belong to genus Pseudomonas by morphological, physiological and biochemical characterizations, and then through 16S rDNA sequence it was identified to be likely Pseudomonas hibiscicola. In addition, the culture mediums, carbon sources and culture conditions of strain SD8 were optimized for maximum production of alkaline protease. Optimum enzyme production (236U/mL when cultured bacteria being at 0.75 mg dry weight/mL fermentation broth) was obtained when the isolate at a 3% inoculum size was grown in LB medium at 20 mL medium/100mL Erlenmeyer flask for 48h culture at 30°C with an initial of pH 7.5. This was the first report of strain Pseudomonas hibiscicola secreting alkaline protease, and the data for its optimal cultural conditions for alkaline protease production has laid a foundation for future exploration for the potential use of SD8 strain for alkaline protease production.

  5. Increasing the Efficiency on Producing Radiology Reports for Breast Cancer Diagnosis by Means of Structured Reports. A Comparative Study.

    Segrelles, J Damian; Medina, Rosana; Blanquer, Ignacio; Martí-Bonmatí, Luis


    Radiology reports are commonly written on free-text using voice recognition devices. Structured reports (SR) have a high potential but they are usually considered more difficult to fill-in so their adoption in clinical practice leads to a lower efficiency. However, some studies have demonstrated that in some cases, producing SRs may require shorter time than plain-text ones. This work focuses on the definition and demonstration of a methodology to evaluate the productivity of software tools for producing radiology reports. A set of SRs for breast cancer diagnosis based on BI-RADS have been developed using this method. An analysis of their efficiency with respect to free-text reports has been performed. The methodology proposed compares the Elapsed Time (ET) on a set of radiological reports. Free-text reports are produced with the speech recognition devices used in the clinical practice. Structured reports are generated using a web application generated with TRENCADIS framework. A team of six radiologists with three different levels of experience in the breast cancer diagnosis was recruited. These radiologists performed the evaluation, each one introducing 50 reports for mammography, 50 for ultrasound scan and 50 for MRI using both approaches. Also, the Relative Efficiency (REF) was computed for each report, dividing the ET of both methods. We applied the T-Student (T-S) test to compare the ETs and the ANOVA test to compare the REFs. Both tests were computed using the SPSS software. The study produced three DICOM-SR templates for Breast Cancer Diagnosis on mammography, ultrasound and MRI, using RADLEX terms based on BIRADs 5th edition. The T-S test on radiologists with high or intermediate profile, showed that the difference between the ET was only statistically significant for mammography and ultrasound. The ANOVA test performed grouping the REF by modalities, indicated that there were no significant differences between mammograms and ultrasound scans, but both

  6. Hairy Root Cultures of Gymnema sylvestre R. Br. to Produce Gymnemic Acid.

    Rajashekar, J; Kumar, Vadlapudi; Veerashree, V; Poornima, D V; Sannabommaji, Torankumar; Gajula, Hari; Giridhara, B


    Gymnema sylvestre R. Br. (Asclepiadaceae) is an endangered species extensively used in the management of diabetes, obesity, and treatment of various diseases. Uncontrolled exploitation to meet the increasing demand and low seed viability hastens the disappearance of the plant from its natural habitat. Hairy root culture provides a suitable alternative for the enhanced production of active principles. The current protocol provides the optimized culture conditions for the establishment of hairy root cultures and elicitation studies and also confirmation of stable integration of A. rhizogenes plasmid T-DNA into host genetic material by PCR and RT-PCR. Furthermore, it also discusses the suitable methods for the extraction procedures, and qualitative and quantitative analysis of gymnemic acid by HPTLC and HPLC.

  7. Characterization of growth hormone and prolactin produced by human pituitary in culture.

    Skyler, J S; Rogol, A D; Lovenberg, W; Knazek, R A


    Fragments of a pituitary tumor from a patient with acromegaly were grown in tissue culture. The tumor secreted both growth hormone and prolactin,which were recovered in high concentrations. The nonpurified hormones were characterized and compared to their respective counterparts obtained by extraction from normal pituitaries obtained at autopsy. The tissue culture and pituitary extracted hormones were eluted from Sephadex G-100 with the same partition coefficients. Growth hormone from both sources showed parallel dose-response displacement curves, by logit-log transformation, in both specific immunoassay and in a specific lymphocyte binding assay. Prolactin from both sources was compared in specific immunoassay using three different antisera. Parallel logit-log displacement curves were seen with one antiserum, while the other two antisera yielded non-parallel curves, indicating structural differences between prolactin from the two sources. Quantitative polyacrylamide gel electrophoresis was performed using multiphasic buffer systems previously developed for characterization of each hormone. By the criteria of joint 95% confidence envelopes of retardation co-efficient and relative free mobility, tissue culture growth hormone and prolactin were indistinguishable from their pituitary-extracted counterparts. This study demonstrates that, prior to purification, tissue culture derived hormone can be characterized by multiple criteria and compared to a standard preparation. Structural differences can be detected, as in the case of prolactin. When the hormones are indistinguishable, as in the case of growth hormone, it becomes worthwhile to increase the scale of tissue cultured production, with the prospect that tissue culture may serve as a source of hormone for both experimental and therapeutic use.

  8. What does it mean to be a man today?: bloke culture and the media.

    Hodgetts, Darrin; Rua, Mohi


    Psychologists have paid scant attention to the positive relationships and community contributions of working class men who are not in trouble, and have focused instead on men who are 'in trouble.' In addressing this oversight, we draw on insights from ethnographic observations, life narrative interviews, photographic techniques and media items, which have been compiled by 12 working class men from a shared community of practice in New Zealand. We illustrate how these men often appropriate aspects from contemporary media deliberations regarding what it means to be a man today in order to make sense of their own lives. The implications of participants' emphasis on friendship, support, familial obligations, and community participation are discussed in relation to the place of working class men in society.

  9. Elaboration of Method of Long-Term Culturing and Selection of Enzyme Producers

    Blieva Raushan


    Full Text Available On the basis of the conducted researches on pectin lyase and proteolytic enzyme biosynthesis by Penicillium and Asperaillus micromycetes we have developed efficient methods for their cultivation and selection. We theoretically substantiated and experimentally confirmed an advantage of growing micromycetes in a new filament-spongy immobilized growth structure on the substrate relative to the traditional method of deep cultivation of free cells in the form of pellets. When comparing a traditional with our innovative method of cultivation, many advantages of the latter are revealed, above all being the possibility of the formation of new highly selective cultures in the long process of their growth with modified culturally - morphological properties.

  10. A cultural take on the links between religiosity, identity, and meaning in life in religious emerging adults.

    Negru-Subtirica, Oana; Tiganasu, Alexandra; Dezutter, Jessie; Luyckx, Koen


    Identity and meaning in life are core developmental assets in emerging adulthood. We analysed how religiosity is related to these intentional strivings in emerging adults enrolled in theological education, by depicting (1) identity strivings and meaning in life accounts in faith narratives (Study 1) and (2) links between personal identity and meaning in life profiles and religious beliefs, behaviours, and subjective experiences (Study 2). Both studies highlighted that a Foreclosed status, with high personal commitment and reduced exploration, was dominant in faith narratives and personal identity profiles. Also, in narratives meaning in life was reflected by a strong focus on presence of meaning through religious insights. Nonetheless, global meaning in life profiles indicated that many emerging adults were searching for a meaning in their lives, while reporting lower levels of presence of meaning. Identity Achievement and High Presence-High Search profiles were linked to the highest levels of subjective, behavioural, and cognitive religiosity. We highlighted the multidimensionality of identity and meaning in life strivings in emerging adults attending theological schools. We pointed out that even in a somewhat foreclosed cultural context (e.g., Romanian Christian Orthodox theological schools), religion represents a dynamic social and ideological context for self-development. Statement of contribution What is already known on this subject? Religious beliefs increase in emerging adults, doubled by decreases in religious behaviours, linked to an adherence to a more personal approach to religion. Religious youth are more committed to their faith and also explore identity and life meaning in relation to their religious strivings. Youth religious exemplars report close links between their religious faith and strivings for meaningful life goals. What does this study add? We investigated Christian Orthodox theology students, for whom religion is a normative dimension of


    Sri Ambarwangi


    Full Text Available This paper is the result of the study and the implementation of learning in the field of the author, especially in the implementation of the study of Arts and Culture in the vocational schools. The purpose of this paper is to show that by learning local culture based on learning objectives the corresponding to the curriculum can be reached. In fact, the local culture also containing more local wisdom is felt directly by the students. The art of Reog, known around Pringapus Semarang Regency, can be used as means of learning arts for students, especially in the activities of appreciation and expressions. The existing values in the art of Reog can be conceptually presented in class, as well as directly through the students’ activities of appreciation and expression in the form of performances in the school environment. Students can easily receive learning materials and can be more expressive while presenting the show. The values that are in the presentation of the art of Reog are social, religion, nationalism, and culture. Students can present Reog with enthusiasm and expressive as a show that has already exist since a long time in the environment of their own, and even many have become the performers of Reog in their neighborhood. Reog has already been part of his life that has the role of self-actualization, expression, social, and cultural. Most of those students feel proud to be a part in the show in his living quarters so that they do not feel ashamed of serving the art in school.

  12. Chain Elongation with Reactor Microbiomes: Open-Culture Biotechnology To Produce Biochemicals

    Angenent, L.T.; Richter, H.; Buckel, W.; Spirito, C.M.; Steinbusch, K.J.J.; Plugge, C.M.; Strik, D.; Grootscholten, T.I.M.; Buisman, C.J.N.; Hamelers, H.V.M.


    Chain elongation into medium-chain carboxylates, such as n-caproate and n-caprylate, with ethanol as an electron donor and with open cultures of microbial consortia (i.e., reactor microbiomes) under anaerobic conditions is being developed as a biotechnological production platform. The goal is to use

  13. High cell density cultures produced by internal retention: application in continuous ethanol fermentation

    Berta Carola Pérez


    Full Text Available Ethanol has provoked great interest due to its potential as an alternative fuel. Nevertheless, fermentation processes must be developed by increasing the low volumetric productivity achieved in conventional cultures (batch or continuous to make this product become economically competitive. This can be achieved by using techniques leading to high cell concentration and reducing inhibition by the end-product. One of the frequently employed methods involves cell recycling. This work thus developed a membrane reactor incorporating a filtration module with 5 u,m stainless steel tubular units inside a 3L stirred jar fermenter for investigating its application in continuous ethanol production. The effects of cell concentration and transmembrane pressure difference on permeate flux were evaluated for testing the filtration module's performance. The internal cell retention system was operated in Saccharomyces cerevisiae continuous culture derived from sucrose, once fermentation conditions had been selected (30 °C, 1.25 -1.75 vvm, pH 4.5. Filter unit permeability was maintained by applying pulses of air. More than 97% of the grown cells were retained in the fermenter, reaching 51 g/L cell concentration and 8.51 g/L.h average ethanol productivity in culture with internal cell retention; this was twice that obtained in a conventional continuous culture. Key words: Membrane reactor, Saccharomyces cerevisiae, alcoholic fermentation, cell recycling.

  14. Beliefs about history, the meaning of historical events and culture of war

    Magdalena Bobowik


    Full Text Available This study examines beliefs concerning the content of history, the meaning of Second World War (WWII and the evaluation of historical events in relation to pro-war attitudes. Participants were 1183 university students from Spain, Portugal, Argentina, Brazil, Peru and Cape Verde. Four supra-level dimensions in the representations of the past were found: History as progress and leaders-oriented, history as focused on justifying calamities, history as violence and catastrophe, and history as meaningless. The prevalent positive beliefs about history were linked with enthusiasm to fight in a future war for one’s country.---Se estudiaron las creencias sobre el contenido de la historia, el significado de la Segunda Guerra Mundial y la evaluación de eventos históricos en relación con una actitud favorable a la guerra. Los participantes fueron 1183 estudiantes universitarios de España, Portugal, Argentina, Brasil, Perú y Cabo Verde. Se encontraron cuatro grandes dimensiones en las representaciones sobre el pasado: la historia como proceso de progreso y dirigida por líderes; la historia compuesta por calamidades que se deben aceptar; la historia como violencia y catástrofes; y, la historia como carente de sentido. La prevalente visión positiva de la historia se asoció a una actitud favorable a luchar en una nueva guerra.

  15. Stable coexistence of two Caldicellulosiruptor species in a de novo constructed hydrogen-producing co-culture

    Rådström Peter


    Full Text Available Abstract Background Mixed culture enrichments have been used frequently for biohydrogen production from different feedstock. In spite of the several advantages offered by those cultures, they suffer poor H2 yield. Constructing defined co-cultures of known H2 producers may offer a better performance than mixed-population enrichments, while overcoming some of the limitations of pure cultures based on synergies among the microorganisms involved. Results The extreme thermophiles Caldicellulosiruptor saccharolyticus DSM 8903 and C. kristjanssonii DSM 12137 were combined in a co-culture for H2 production from glucose and xylose in a continuous-flow stirred tank reactor. The co-culture exhibited a remarkable stability over a period of 70 days under carbon-sufficient conditions, with both strains coexisting in the system at steady states of different dilution rates, as revealed by species-specific quantitative PCR assays. The two strains retained their ability to stably coexist in the reactor even when glucose was used as the sole growth-limiting substrate. Furthermore, H2 yields on glucose exceeded those of either organism alone under the same conditions, alluding to a synergistic effect of the two strains on H2 production. A maximum H2 yield of 3.7 mol (mol glucose-1 was obtained by the co-culture at a dilution rate of 0.06 h-1; a higher yield than that reported for any mixed culture to date. A reproducible pattern of population dynamics was observed in the co-culture under both carbon and non-carbon limited conditions, with C. kristjanssonii outgrowing C. saccharolyticus during the batch start-up phase and prevailing at higher dilution rates. A basic continuous culture model assuming the ability of C. saccharolyticus to enhance the growth of C. kristjanssonii could mimic the pattern of population dynamics observed experimentally and provide clues to the nature of interaction between the two strains. As a proof, the cell-free growth supernatant of C

  16. Producing Films, Producing Meanings: Some Theoretical Reflections

    Giorgio Grignaffini


    Full Text Available The article aims to stimulate greater attention by  semiotics of cinema upon production practices and preparatory materials for the film, as yet not so much studied compared to the dynamics that the texts themselves activate on the audiences. This proposed extension of the field of research, is thorough in its theoretical premises in two phases. First is contextualized in relation to the practice of "traditional" semiotic analysis; then, using the semiotic tools developed by Eric Landowski, in particular by exploiting the concept of "adjustment" developed in the framework of reflection on how subjects interact, are highlighted fields of application (e.g. the work of the directors with the actors and heuristic potential benefits.

  17. Effect of airstream velocity on mean drop diameters of water sprays produced by pressure and air atomizing nozzles. [for combustion studies

    Ingebo, R. D.


    A scanning radiometer was used to determine the effect of airstream velocity on the mean drop diameter of water sprays produced by pressure atomizing and air atomizing fuel nozzles used in previous combustion studies. Increasing airstream velocity from 23 to 53.4 meters per second reduced the Sauter mean diameter by approximately 50 percent with both types of fuel nozzles. The use of a sonic cup attached to the tip of an air assist nozzle reduced the Sauter mean diameter by approximately 40 percent. Test conditions included airstream velocities of 23 to 53.4 meters per second at 293 K and atmospheric pressure.

  18. [Therapeutic potential of secondary metabolites produced in the hairy roots cultures].

    Kowalczyk, Tomasz; Łucka, Marta; Szemraj, Janusz; Sakowicz, Tomasz


    Plants have always been a source of many valuable substances for humans. Growing advancement of methods of modern biotechnology, combined with genetic engineering techniques, gradually increase the variety of compounds obtained, the number of plant species used and the production efficiency. Consequently, there is an undebatable interest in biotechnological production of such compounds, especially those pharmacologically active, that can be used in treatment of neoplastic, viral, and many other types of diseases. Most of these compounds represent a diverse group of secondary metabolites. One of the effective ways of obtaining such molecules is the utilization of hairy roots cultures. The advantages of such systems make them an attractive method of obtaining important plant-derived compounds, creating an interesting alternative to other methods, including the cell suspension cultures or expensive chemical syntheses.

  19. Responsive hydrogels produced via organic sol-gel chemistry for cell culture applications.

    Patil, Smruti; Chaudhury, Pulkit; Clarizia, Lisa; McDonald, Melisenda; Reynaud, Emmanuelle; Gaines, Peter; Schmidt, Daniel F


    In this study, we report the synthesis of novel environmentally responsive polyurea hydrogel networks prepared via organic sol-gel chemistry and demonstrate that the networks can stabilize pH while releasing glucose both in simple aqueous media and in mammalian cell culture settings. Hydrogel formulations have been developed based on the combination of an aliphatic triisocyanate with pH-insensitive amine functional polyether and pH-sensitive poly(ethyleneimine) segments in a minimally toxic solvent suitable for the sol-gel reaction. The polyether component of the polyurea network is sufficiently hydrophilic to give rise to some level of swelling independent of environmental pH, while the poly(ethyleneimine) component contains tertiary amine groups providing pH sensitivity to the network in the form of enhanced swelling and release under acidic conditions. The reaction of these materials to form a network is rapid and requires no catalyst. The resultant material exhibits the desired pH-responsive swelling behavior and demonstrates its ability to simultaneously neutralize lactic acid and release glucose in both cell-free culture media and mammalian cell culture, with no detectable evidence of cytotoxicity or changes in cell behavior, in the case of either SA-13 human hybridomas or mouse embryonic stem cells. Furthermore, pH is observed to have a clear effect on the rate at which glucose is released from the hydrogel network. Such characteristics promise to maintain a favorable cell culture environment in the absence of human intervention. Copyright © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

  20. Effect of culture conditions on the growth of biomass Yarrowia lipolytica - producing protein feed

    O. S. Korneeva


    Full Text Available Fodder yeast is highly valuable protein-vitamin products. Protein digestibility by yeast and amino acid content, superior proteins of animal origin. Fodder yeast protein digested in animals by 95 %. The biological value of yeast protein is determined by the presence of a significant amount of essential amino acids. Moreover, yeast cells contain many vitamins microelement and a significant amount of fat, in which the predominant unsaturated fatty acid. Currently, fodder yeast successfully used in livestock and poultry, so the demand for them is increasing every year. For the production of fodder yeast using a yeast having the necessary technological properties: the ability of rapid growth in aerobic conditions to form protein, amino acids and vitamins, resistant crop production, the development of resistance to foreign microorganisms. Intensive education yeast biomass contributes to a number of conditions, including pH, temperature and aeration of the culture occupy an important place. The main criterion for comparison and selection of a culture medium for this is the speed of its growth and ability to assimilate all of the nutrients with high economic factor. It depends on the performance of the enterprise, energy consumption and other technical - economic performance. The effect of pH of the medium on the biomass accumulation of yeast Yarrowia lipolytica. Found that at pH 5,2 - 5,5 observed maximum growth rate of the yeast cells. The effect of temperature on the accumulation of yeast biomass. The temperature of the culture medium determines the intensity of metabolism in cells. It was found that the optimal growth temperature of the culture Yarrowia lipolytica is 33 0C. The effect of aeration on the growth rate of yeast cells. Tro-established that the maximum increase of biomass was obtained with the aeration of 70 cm3 /cm3hrs.

  1. Novel Approach to Tar Removal from Biomass Producer Gas by Means of a Nickel-Based Catalyst

    Vosecký, M.; Kameníková, P.; Pohořelý, M.; Skoblja, S.; Punčochář, M.

    The nickel-based catalyst was exposed to the raw gas from gasification of woody biomass with air in a fluidized-bed. After dust removal on a barrier filter and sulphur compounds capture, namely H2S, on an active sorbent made of CuO and ZnO, higher hydrocarbons as tar components were decomposed/reformed on aNi-catalyst. Steam reforming reactions led to decomposition of tar and all hydrocarbons higher than CH4 into mainly H2 and CO which further underwent reaction with steam via the water gas shift reaction to CO2. The reforming reactions caused approximately 10-20 % decrease in the lower heating values of the producer gas from the inlet values 5.0-6.5 MJ m-3. The gas yield increased fromvalues 2.4-2.6 m3 kg-1 to values 2.8-3.0 m3 kg-1 on dry biomass basis. The chosen tar removal concept based on combination of dolomite in the fluidized-bed with the secondary catalytic reactor was proved by 20 hours long experiment in which the finaltar content below 30 mg m-3 was attained corresponding to more than 97 % tar conversion. H2S content in producer gas was expected to be below 100 vol. ppm, bulk of which was captured on the sorbent. Only limited deactivation of thecatalyst by sulphur compounds was found in the front of the catalyst bed where sulphur content was determined as high as 173 wt. ppm compared to 22 wt. ppm in the fresh sample.

  2. A Comparative Study of Cultural--loaded Meaning of English and Chinese Fruit Words in View of Cultural Psychology%从文化心理角度看英汉水果类词的文化义



    Cultural- loaded meaning is the reflection of culture. Different cultural language environments have some common cultural--loaded meaning, but also have particular cultural-- loaded meaning. Combining the living examples of fruit words, and with an eye to the relationship between cultural- loaded meaning and cultural psychology' this ,oaper analyses the cultural differences in different cultural environment of cultural--loaded meaning., and therefore grasp the language phenomenon from the view of culture.%文化义是文化色彩的再现。不同文化语境既存在共有的文化义,也存在特有的文化义。本文结合水果类词的实例,着眼于文化义与文化心理的关系,分析其文化义在不同文化语境下体现的文化差异,从文化的角度来把握语言现象。

  3. Community health workers as cultural producers in addressing gender-based violence in rural South Africa.

    de Lange, Naydene; Mitchell, Claudia


    South Africa has been experiencing an epidemic of gender-based violence (GBV) for a long time and in some rural communities health workers, who are trained to care for those infected with HIV, are positioned at the forefront of addressing this problem, often without the necessary support. In this article, we pose the question: How might cultural production through media making with community health workers (CHWs) contribute to taking action to address GBV and contribute to social change in a rural community? This qualitative participatory arts-based study with five female CHWs working from a clinic in a rural district of South Africa is positioned as critical research, using photographs in the production of media posters. We offer a close reading of the data and its production and discuss three data moments: CHWs drawing on insider cultural knowledge; CHWs constructing messages; and CHWs taking action. In our discussion, we take up the issue of cultural production and then offer concluding thoughts on 'beyond engagement' when the researchers leave the community.

  4. Microanalysis characterization of bioactive protein-bound polysaccharides produced by Amanita ponderosa cultures.

    Salvador, Cátia; Martins, M Rosário; Caldeira, A Teresa


    Different compounds of edible mushrooms are responsible for their bioactivity. The ability to synthesize polysaccharides, namely protein-polysaccharide (PPS) complexes, is related to the antioxidant capacity of these compounds and present great interest in preventing a number of diseases, including cancer, cardiovascular and auto-immune diseases, and accelerated aging. Amanita ponderosa are wild edible mushrooms that grow in Mediterranean "montado" areas [Portuguese name given to cork oak (Quercus suber) and holm oak (Quercus ilex) forests]. The aim of this study was to evaluate the production of PPS complexes obtained from A. ponderosa cultures using a new microanalytical approach to quickly and easily monitor the production process. Microanalysis using Fourier-transform infrared using attenuated total reflection and Raman spectroscopy of PPS samples showed spectra compatible with identification of this type of compound in culture extracts. PPS separated by size-exclusion chromatography showed seven main complexes. Molecular weights of the main PPS complexes isolated from cultures ranged between 1.5 and 20 kDa and did not present toxicity against Artemia salina, demonstrating the potential of A. ponderosa as a source of biologically active compounds with nutraceutical value. Application of this microanalytical approach to monitoring the production of PPS compounds can be successfully applied in biotechnological processes.

  5. South Brazilian wines: culturable yeasts associated to bottled wines produced in Rio Grande do Sul and Santa Catarina.

    Ramírez-Castrillón, Mauricio; Mendes, Sandra Denise Camargo; Valente, Patricia


    A comprehensive understanding of the presence and role of yeasts in bottled wines helps to know and control the organoleptic quality of the final product. The South Region of Brazil is an important wine producer, and the state of "Rio Grande do Sul" (RS) accounts for 90% of Brazilian wines. The state of "Santa Catarina" (SC) started the production in 1975, and is currently the fifth Brazilian producer. As there is little information about yeasts present in Brazilian wines, our main objective was to assess the composition of culturable yeasts associated to bottled wines produced in RS and SC, South of Brazil. We sampled 20 RS and 29 SC bottled wines produced between 2003 and 2011, and we isolated culturable yeasts in non-selective agar plates. We identified all isolates by sequencing of the D1/D2 domain of LSU rDNA or ITS1-5.8 S-ITS2 region, and comparison with type strain sequences deposited in GenBank database. Six yeast species were shared in the final product in both regions. We obtained two spoilage yeast profiles: RS with Zygosaccharomyces bailii and Pichia membranifaciens (Dekkera bruxellensis was found only in specific table wines); and SC with Dekkera bruxellensis and Pichia manshurica. Knowledge concerning the different spoilage profiles is important for winemaking practices in both regions.

  6. To Introduce a Cultural Sense in Translation——A Brief Study of Non-equivalence in the Associated Meanings of English and Chinese Words


    This paper attempts to demonstrate the importance of intrpdicomg cultural sense in translation practice,English to Chinese in particular,by examining the problem of non-equivalence at word level in associated meanings and to unveil the cultural aspects projected within.It is also argued that greater importance should be attached to the cultural translation and flexible strategies should be adopted in dealing with the problems of non-equivalence in the associated meanings of words of different cultures.

  7. Method for Producing Non-Neoplastic, Three Dimensional, Mammalian Tissue and Cell Aggregates Under Microgravity Culture Conditions and the Products Produced Therefrom

    Goodwin, Thomas J. (Inventor); Wolf, David A. (Inventor); Spaulding, Glenn F. (Inventor); Prewett, Tracey L. (Inventor)


    Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural, and blood tissue. The cells are grown in vitro under microgravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.

  8. Electrical, Mechanical, and Thermal Properties of LDPE Graphene Nanoplatelets Composites Produced by Means of Melt Extrusion Process

    Karolina Gaska


    Full Text Available Composites of LDPE filled with different amounts of graphene nanoplatelets (GnP were prepared in form of films by means of precoating technique and single screw melt-extrusion using two types of screws, compression and mixing. This manufacturing process imposes strong anisotropy on the sample’s morphology, in which the nanoplatelets become oriented along the extrusion direction. Such orientation of GnP in LDPE matrix is confirmed by scanning electron microscopy observations and it yields unique electrical properties. As compared to pure LDPE, significant reductions of the through-plane conductivity are found for the composites at relatively low electric fields (<20 kV/mm at low filler concentrations. Above the field level of 20 kV/mm, a crossover effect is observed that results in a strong field dependency of the conductivity where the non-linear behavior starts to dominate. Moreover, differential scanning calorimetry (DSC results indicate a decrease in polymer crystallinity of the composite matrix with increasing filler content, whereas thermogravimetric (TG analysis shows a slight increase in the material’s thermal stability. Application of GnP also leads to improvement of mechanical properties, manifested by the increase of Young’s modulus and tensile strength in both types of samples.

  9. Availability of pearl producing marine bivalves in south-eastern coast of Bangladesh and culture potentialities

    Md Ataur Rahman


    Full Text Available The present study was conducted during September 2014 to July 2015 to identify the pearl bearing bivalves in south-eastern coast of Bangladesh and culture potentialities of marine oyster in captivity based on field investigation. A total of 7 pearl bearing bivalve species were identified in the coast with a salinity of 18-34 ppt, pH 8.1-8.3 and water depth ranged 0.2-2.0 meter in their habitat. From the collected bivalves, most abundant oyster species windowpane oyster, Placuna placenta (Linnaeus, 1758 was reared in fiber glass tanks with seawater for a period of 6 months. During rearing highest survival rate of 88% was observed in T1 with sandy and gravel substratum and lowest survival rate of 78% was found in T2 with muddy substratum. Average temperature and salinity were varied between 24 °C-25 °C and 21-26 ppt respectively. From the reared oyster, highest 54 nos. small pearls in the month of April and lowest 7 pearls in December from a single P. placenta were obtained. The study proved that pearls can be obtained from the marine oysters in captivity in Bangladesh, and this offers large scale culture potentialities in our coast.

  10. Mutagenesis breeding of high echinocandin B producing strain and further titer improvement with culture medium optimization.

    Zou, Shu-Ping; Zhong, Wei; Xia, Chao-Jie; Gu, Ya-Nan; Niu, Kun; Zheng, Yu-Guo; Shen, Yin-Chu


    A combination of microbial strain improvement and statistical optimization is investigated to maximize echinocandin B (ECB) production from Aspergillus nidulans ZJB-0817. A classical sequential mutagenesis was studied first by using physical (ultraviolet irradiation at 254 nm) and chemical mutagens (lithium chloride and sodium nitrite). Mutant strain ULN-59 exhibited 2.1-fold increase in ECB production to 1583.1 ± 40.9 mg/L when compared with the parent strain (750.8 ± 32.0 mg/L). This is the first report where mutagenesis is applied in Aspergillus to improve ECB production. Further, fractional factorial design and central composite design were adopted to optimize the culture medium for increasing ECB production by the mutant ULN-59. Results indicated that four culture media including peptone, K2HPO4, mannitol and L-ornithine had significant effects on ECB production. The optimized medium provided another 1.4-fold increase in final ECB concentration to 2285.6 ± 35.6 mg/L compared to the original medium. The results of this study indicated the combined application of a classical mutation and medium optimization can improve effectively ECB production from A. nidulans and could be a promising tool to improve other secondary metabolites production by fungal strains.

  11. Transcript and metabolite profiling in cell cultures of 18 plant species that produce benzylisoquinoline alkaloids.

    Farrow, Scott C; Hagel, Jillian M; Facchini, Peter J


    Benzylisoquinoline alkaloids (BIAs) are a large and diverse group of ~2500 specialized metabolites found predominantly in plants of the order Ranunculales. Research focused on BIA metabolism in a restricted number of plant species has identified many enzymes and cognate genes involved in the biosynthesis of compounds such as morphine, sanguinarine and berberine. However, the formation of most BIAs remains uncharacterized at the molecular biochemical level. Herein a compendium of sequence- and metabolite-profiling resources from 18 species of BIA-accumulating cell cultures was established, representing four related plant families. Our integrated approach consisted of the construction of EST libraries each containing approximately 3500 unigenes per species for a total of 58,787 unigenes. The EST libraries were manually triaged using known BIA-biosynthetic genes as queries to identify putative homologs with similar or potentially different functions. Sequence resources were analyzed in the context of the targeted metabolite profiles obtained for each cell culture using electrospray-ionization and collision-induced dissociation mass spectrometry. Fragmentation analysis was used for the identification or structural characterization coupled with the relative quantification of 72 BIAs, which establishes a key resource for future work on alkaloid biosynthesis. The metabolite profile obtained for each species provides a rational basis for the prediction of enzyme function in BIA metabolism. The metabolic frameworks assembled through the integration of transcript and metabolite profiles allow a comparison of BIA metabolism across several plant species and families. Taken together, these data represent an important tool for the discovery of BIA biosynthetic genes.

  12. Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture.

    Onitsuka, Masayoshi; Kawaguchi, Akira; Asano, Ryutaro; Kumagai, Izumi; Honda, Kohsuke; Ohtake, Hisao; Omasa, Takeshi


    N-Glycosylation of therapeutic antibodies contributes not only to their biological function, but also to their stability and tendency to aggregate. Here, we investigated the impact of the glycosylation status of an aggregated antibody that accumulated during the bioreactor culture of Chinese hamster ovary cells. High-performance liquid chromatography analysis showed that there was no apparent difference in the glycosylation patterns of monomeric, dimeric, and large aggregated forms of the antibody. In contrast, lectin binding assays, which enable the total amounts of specific sugar residues to be detected, showed that both galactose and fucose residues in dimers and large aggregates were reduced to 70-80% of the amount in monomers. These results strongly suggest that the lack of N-linked oligosaccharides, a result of deglycosylation or aglycosylation, occurred in a proportion of the dimeric and large aggregated components. The present study demonstrates that glycosylation heterogeneities are a potential cause of antibody aggregation in cell culture of Chinese hamster ovary cells, and that the lack of N-glycosylation promotes the formation of dimers and finally results in large aggregates.

  13. Effect of resveratrol supplementation during culture on the quality and cryotolerance of bovine in vitro produced embryos.

    Salzano, A; Albero, G; Zullo, G; Neglia, G; Abdel-Wahab, A; Bifulco, G; Zicarelli, L; Gasparrini, B


    The aim of the study was to evaluate whether resveratrol supplementation of bovine culture medium improves in vitro blastocyst development, embryo cryotolerance and cell numbers. Abattoir-derived oocytes were matured and fertilized in vitro according to standard procedure. Twenty hours after IVF, zygotes were cultured in SOF medium, supplemented with 0 (control, n=439), 0.25μM (n=422), 0.5μM (n=447) and 1μM resveratrol (n=416). On Day 7 (IVF=Day 0) blastocysts were vitrified by cryotop in 16.5% ethylene glycol, 16.5% dimethyl sulfoxide and 0.5M sucrose. Development rate, i.e. the percentage of embryos resuming development to reach a more advanced stage, and hatching rate were evaluated after 24 and 48h culture. Blastocysts cultured with (0.5μM) and without resveratrol underwent differential staining to count inner cell mass (ICM) and trophectoderm (TE) cells. Resveratrol during culture did not increase blastocyst yields (57.1, 57.7, 59.2 and 46.6%, respectively in 0, 0.25, 0.5 and 1μM resveratrol). However, 0.5μM resveratrol improved embryo cryotolerance compared to the control, as indicated by higher development rates (67.3% vs 50.3%, respectively; Pculture. Blastocysts produced in the control and in 0.5μM resveratrol groups had similar numbers of ICM (34.1 and 36.4, respectively), TE (88.1 and 85.3, respectively) and total (122.2 and 121.7, respectively) cells. In conclusion, low levels of resveratrol during in vitro culture improve the quality of IVP bovine embryos, as indicated by their increased resistance to cryopreservation.

  14. Importance in dairy technology of bacteriocins produced by dairy starter cultures

    Bedia Şimşek


    Full Text Available Bacteriocins produced by Lactic acid bacteria (LAB and propionic acid bacteria (PAB are heterogeneous group of peptide inhibitors which include lantibiotics (class I, e. g. nisin, small heat-stable peptides (class II, e. g. pediocin PA-1 and large heat-labile proteins (class III, e. g. helveticin J. Many bacteriocins belonging to the first two groups can be successfully used to inhibit undesirable microorganisms in foods, but only nisin is produced industrially and is used as a food preservative. LAB and PAB develops easily in milk and milk products. LAB and PAB growth in dairy products can cause microbial interference to spoilage and pathogenic bacteria through several metabolits, specially bacteriocins. The review deals with the description of milk-borne bacteriocins and their application in milk and milk products either to extend the shelf life or to inhibit milk pathogens.

  15. Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate

    Ghiringhelli, Pablo Daniel; Hours,Roque Alberto; Chesini, Mariana; Neila, Lorena Paola; Fratebianchi de la Parra, Dante; Rojas,Natalia Lorena; Contreras Esquivel, Juan Carlos; Cavalitto, Sebastian Fernando


    Inulinases have been extracted and characterized from inulin-storing tissues,however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts a...

  16. A Rapid Culture Technique Produces Functional Dendritic-Like Cells from Human Acute Myeloid Leukemia Cell Lines

    Jian Ning


    Full Text Available Most anti-cancer immunotherapeutic strategies involving dendritic cells (DC as vaccines rely upon the adoptive transfer of DC loaded with exogenous tumour-peptides. This study utilized human acute myeloid leukemia (AML cells as progenitors from which functional dendritic-like antigen presenting cells (DLC were generated, that constitutively express tumour antigens for recognition by CD8+ T cells. DLC were generated from AML cell lines KG-1 and MUTZ-3 using rapid culture techniques and appropriate cytokines. DLC were evaluated for their cell-surface phenotype, antigen uptake and ability to stimulate allogeneic responder cell proliferation, and production of IFN-γ; compared with DC derived from normal human PBMC donors. KG-1 and MUTZ-3 DLC increased expression of CD80, CD83, CD86, and HLA-DR, and MUTZ-3 DLC downregulated CD14 and expressed CD1a. Importantly, both KG-1 and MUTZ-3-derived DLC promoted proliferation of allogeneic responder cells more efficiently than unmodified cells; neither cells incorporated FITC-labeled dextran, but both stimulated IFN-γ production from responding allogeneic CD8+ T cells. Control DC produced from PBMC using the FastDC culture also expressed high levels of critical cell surface ligands and demonstrated good APC function. This paper indicates that functional DLC can be cultured from the AML cell lines KG-1 and MUTZ-3, and FastDC culture generates functional KG-1 DLC.

  17. The nutritional quality of drum-dried algae produced in open door mass culture.

    Saleh, A M; Hussein, L A; Abdalla, F E; el-Fouly, M M; Shaheen, A B


    The yield of three large scale cultures of Scenedesmus acutus, Chlorella vulgaris, and Coelastrum proboscideum was drum dried. The amino acid composition of the three species proved to compare well with the FAO (11) amino acid pattern except for methionine and isoleucine. Bio-assay evaluation of the three algal proteins gave the following values: protein efficiency ratio, 1.9-2.1; net protein ratio, 2,4-2.8; biological value, 75-78; digestibility coefficient 88-89; and calculated net protein utilization 67-69. Total nucleic acid content was about 4%. Uric acid content in the plasma of rats fed Scenedesmus, Chlorella, and Coelastrum diets was significantly higher (P less than 0.05) than in plasma of rats fed on a casein diet.

  18. New Additive for Culture Media for Rapid Identification of Aflatoxin-Producing Aspergillus Strains

    Fente, C. A.; Ordaz, J. Jaimez; Vázquez, B. I.; Franco, C. M.; Cepeda, A.


    A new reliable, fast, and simple method for the detection of aflatoxigenic Aspergillus strains, consisting of the addition of a cyclodextrin (a methylated β-cyclodextrin derivative) to common media used for testing mycotoxin production ability, was developed. We propose the use of this compound as an additive for fungal culture media to enhance the natural fluorescence of aflatoxins. The production of aflatoxins coincided with the presence of a bright blue or blue-green fluorescent area surrounding colonies when observed under long-wavelength (365-nm) UV light after 3 days of incubation at 28°C. The presence of aflatoxins was confirmed by extracting the medium with chloroform and examining the extracts by high-pressure liquid chromatography with fluorescence detection. PMID:11571194

  19. A comparison of Culture-Free Self-Esteem Scale means from different child and adolescent groups.

    Holaday, M; Callahan, K; Fabre, L; Hall, C; MacDonald, N; Mundy, M A; Owens, B; Plappert, H


    The Culture-Free Self-Esteem Inventory (CFSEI-2) was administered to 7 groups of children: 84 White Catholic school students from a New Orleans suburb, 78 White rural public school students from Virginia, 62 Hispanic Migrant student from Florida, 90 Aboriginal and White students from an isolated Canadian community, 199 African American students attending an inner city school, 60 Hispanic and White international students from Venezuela, and 61 Innuit students from isolated community in Labrador. The four elder groups also wrote three words to describe themselves (the Adjective Generation Technique [AGT]). Significant differences in responding between groups were found on all CFSEI-2 scales and for AGT favorability means. Although several possible reasons for these results are discussed, we conclude that the CFSEI-2 is not culture-free. Recommendations are: change the title of the test to avoid misrepresentation, limit test usage to elementary school children, develop an adolescent version with age appropriate language, and construct local norms before using the CFSEI-2 to make decisions about a child's self-esteem. To determine relevance of scores, a team of professionals and lay persons should review items from this or any test given to children who may be different from the normative or standardization group.

  20. Molecular characterization of viruses from clinical respiratory samples producing unidentified cytopathic effects in cell culture.

    Abed, Yacine; Boivin, Guy


    The sequence-independent single primer amplification (SISPA) method was performed to identify a virus in 17 clinical respiratory samples producing uncharacterized cytopathic effects in LLC-MK2 cells. Sequence analysis of 600-1600 bp amplicons allowed the identification of six viruses (one influenza C, two parechovirus-3 and three cardioviruses). Genomic sequences of the cardioviruses showed similarities with those of the recently-described Saffold virus strain although significant variation was present in the viral surface EF and CD loops. These results demonstrate the usefulness of SISPA for identifying emerging viruses and also known viruses not easily identified by standard virological methods.

  1. Molecular Characterization of Viruses from Clinical Respiratory Samples Producing Unidentified Cytopathic Effects in Cell Culture

    Guy Boivin


    Full Text Available The sequence-independent single primer amplification (SISPA method was performed to identify a virus in 17 clinical respiratory samples producing uncharacterized cytopathic effects in LLC-MK2 cells. Sequence analysis of 600-1600 bp amplicons allowed the identification of six viruses (one influenza C, two parechovirus-3 and three cardioviruses. Genomic sequences of the cardioviruses showed similarities with those of the recently-described Saffold virus strain although significant variation was present in the viral surface EF and CD loops. These results demonstrate the usefulness of SISPA for identifying emerging viruses and also known viruses not easily identified by standard virological methods.

  2. The Definition of Wuling (Five Ridges) and Its Cultural Symbolic Meanings%五岭界说及其文化象征意义

    韦祖松; 金强


    Having collected extensive materials, the author makes careful textual criticism on the definition of Wuling and concludes that in history Wuling is not only a protective screen but also a symbolic meaning of the cultural barrier.

  3. Culture of a high-chlorophyll-producing and halotolerant Chlorella vulgaris.

    Nakanishi, Koichi; Deuchi, Keiji


    In order to increase the value of freshwater algae as raw ingredients for health foods and feed for seawater-based farmed fish, we sought to breed high-chlorophyll halotolerant Chlorella with the objective of generating strains with both high chlorophyll concentrations (≥ 5%) and halotolerance (up to 1% NaCl). We used the Chlorella vulgaris K strain in our research institute culture collection and induced mutations with UV irradiation and acriflavine which is known to effect mutations of mitochondrial DNA that are associated with chlorophyll production. Screenings were conducted on seawater-based "For Chlorella spp." (FC) agar medium, and dark-green-colored colonies were visually selected by macroscopic inspection. We obtained a high-chlorophyll halotolerant strain (designated C. vulgaris M-207A7) that had a chlorophyll concentration of 6.7% (d.m.), a level at least three-fold higher than that of K strain. This isolate also exhibited a greater survival rate in seawater that of K strain.

  4. Cordyceps pruinosa produces cordycepin and N6-(2-hydroxyethyl-adenosine in culture

    Meng Zebin


    Full Text Available Cordyceps species are entomophagous pathogens with medicinal properties, mostly linked to cordycepin and N6- (2-hydroxyethyl-adenosine (HEA. An isolate of Cordyceps pruinosa (GZUCC 8552 was obtained from a fruiting body formed on the cocoon a Limacodidae insect collected in Guizhou Province, China. Morphological and molecular analysis (combined 5.8S ITS, RPB1 and 18S RNA confirmed the species to be Cordyceps pruinosa. Metabolites of the isolate grown in liquid static and solid-state media were established by HPLC-MS. Cordycepin (5.311 mg/g and HEA (0.558 mg/g were produced by this strain. This is the first record of cordycepin from an isolate of Cordyceps pruinosa. As Cordyceps pruinosa is a good source of cordycepin and HEA, it could be used as an alternative to the over-collected Cordyceps sinensis.

  5. Screening of phenylpyruvic acid producers and optimization of culture conditions in bench scale bioreactors.

    Coban, Hasan B; Demirci, Ali; Patterson, Paul H; Elias, Ryan J


    Alpha keto acids are deaminated forms of amino acids that have received significant attention as feed and food additives in the agriculture and medical industries. To date, their production has been commonly performed at shake-flask scale with low product concentrations. In this study, production of phenylpyruvic acid (PPA), which is the alpha keto acid of phenylalanine was investigated. First, various microorganisms were screened to select the most efficient producer. Thereafter, growth parameters (temperature, pH, and aeration) were optimized in bench scale bioreactors to maximize both PPA and biomass concentration in bench scale bioreactors, using response surface methodology. Among the four different microorganisms evaluated, Proteus vulgaris was the most productive strain for PPA production. Optimum temperature, pH, and aeration conditions were determined as 34.5 °C, 5.12, and 0.5 vvm for PPA production, whereas 36.9 °C, pH 6.87, and 0.96 vvm for the biomass production. Under these optimum conditions, PPA concentration was enhanced to 1,054 mg/L, which was almost three times higher than shake-flask fermentation concentrations. Moreover, P. vulgaris biomass was produced at 3.25 g/L under optimum conditions. Overall, this study demonstrated that optimization of growth parameters improved PPA production in 1-L working volume bench-scale bioreactors compared to previous studies in the literature and was a first step to scale up the production to industrial production.

  6. Baculovirus Coinfection Strategy for Improved Galactosylation of Recombinant Glycoprotein Produced by Insect Cell Culture

    Ney, Yap Wei; Rahman, Badarulhisam Abdul; Aziz, Azila Abdul

    Baculovirus Expression Vector System (BEVS) is widely used for the production of recombinant glycoproteins, but it is not ideal for pharmaceutical glycoprotein production due to incomplete glycosylation. The factors that ensure successful glycosylation are the presence of sufficient amount of glycosyltransferases, sugar nucleotides as the substrate donor and the recombinant protein as the substrate acceptor. In this study, we analyzed the galactosylation process by the introduction of ß-1,4galactosyltransferase (ß-1,4GalT) as the glycosyltransferase of interest and uridine-5`-diphosphogalactose (UDP-Gal) as the substrate donor. Recombinant human transferrin (rhTf) as a model protein was used as the substrate acceptor. Insect cell lines have been reported to produce a small amount of ß-1,4GalT and thus insufficient for effective galactosylation. In this study, we developed a method to produce galactosylated rhTf and optimized the expression of rhTf with better N-glycan quality. Recombinant ß-1,4GalT was introduced during protein expression by the coinfection of the BEVS with baculovirus carrying bovine ß-1,4GalT. To evaluate the extent of galactosylation by the coinfection strategy, a binding assay was established. In this binding assay, glycoprotein acceptor was absorbed onto ELISA plate surface. A lectin known as Ricinus communis agglutinin-I (RCA-I) labeled with peroxidase, was added and allowed to recognize Gal ß1>4GlcNAc group on the N-glycan of the glycoprotein, followed by appropriate color reaction measurements. Coexpression between rhTf and ß-1,4GalT did not show encouraging results due to the reduction of UDP-Gal upon baculovirus infection. This interesting finding suggested that the introduction of ß-1,4GalT alone was not sufficient for successful galactosylation. Alternatively, post harvest glycosylation method strategy seems to be a promising technique in the improvement of glycoprotein quality.

  7. From Scribbles to Meanings: Social Interaction in Different Cultures and the Emergence of Young Children's Early Drawing

    Pinto, Giuliana; Accorti Gamannossi, Beatrice; Cameron, Catherine Ann


    The cultural components of drawing allow one to consider it a symbolic form of cultural communication. The behavioural and cognitive mechanisms involved in the cultural transmission of symbolic communications are situated in an environment embedded in cultural-historical features that should be taken into account, as they give rise to variations…

  8. Screening on DHA-producing Antarctic bacteria N- 6 and its cultural conditions

    Zhang Botao; Miao Jin-lai; Hui Hanxing; Wang Qing


    In Antarctic, the geography and climate differs from those in other places,and the bacteria there have adapted well to the environment there. Two hundred strains of bacteria were isolated from the sea ice in Antarctica. The bacteria were screened for DHA by means of GC, with fish oil as the standard. Seven strains containing DHA or EPA were obtained, among which the strain of No. N-6 was outstanding. And the component of DHA was identified by GC-MS. The relative content of DHA in N-6 was 8.72%, and total lipids in dry bacteria was 22.54%. The effects of some factors, including temperature, salinity and pH value, on the growth and DHAcontent of strain N-6 were studied. The results show that the DHA-content is relatively high when in low temperature and high pH, and the bacterium is psychrophilic, alkalophilic.

  9. Spectral fluorescence signature techniques and absorption measurements for continuous monitoring of biofuel-producing microalgae cultures

    Martín de la Cruz, M. C.; Gonzalez Vilas, L.; Yarovenko, N.; Spyrakos, E.; Torres Palenzuela, J. M.


    Biofuel production from microalgae can be both sustainable and economically viable. Particularly in the case of algal growth in wastewater an extra benefit is the removal or biotransformation of pollutants from these types of waters. A continuous monitoring system of the microalgae status and the concentration of different wastewater contaminants could be of great help in the biomass production and the water characterisation. In this study we present a system where spectral fluorescence signature (SFS) techniques are used along with absorption measurements to monitor microalgae cultures in wastewater and other mediums. This system aims to optimise the microalgae production for biofuel applications or other uses and was developed and tested in prototype indoor photo-bioreactors at the University of Vigo. SFS techniques were applied using the fluorescence analyser INSTAND-SCREENER developed by Laser Diagnostic Instruments AS. INSTAND-SCREENER permits wavelength scanning in two modes, one in UV and another in VIS. In parallel, it permits the on-line monitoring and rapid analysis of both water quality and phytoplankton status without prior treatment of the sample. Considering that different contaminants and microalgae features (density, status etc.) have different spectral signatures of fluorescence and absorption properties, it is possible to characterise them developing classification libraries. Several algorithms were used for the classification. The implementation of this system in an outdoor raceway reactor in a Spanish wastewater treatment plant is also discussed. This study was part of the Project EnerBioAlgae (, which was funded by the Interreg SUDOE and led by the University of Vigo.

  10. Outgrowth inhibition of Clostridium beijerinckii spores by a bacteriocin-producing lactic culture in ovine milk cheese.

    Garde, Sonia; Avila, Marta; Arias, Ramón; Gaya, Pilar; Nuñez, Manuel


    In the manufacture of model cheeses, ovine milk was deliberately contaminated with spores of Clostridium beijerinckii INIA 63, a wild isolate from Manchego cheese with late blowing defect, and inoculated with nisin- and lacticin 481-producing Lactococcus lactis subsp. lactis INIA 415 as starter, to test its potential to prevent the late blowing defect, or with L. lactis subsp. lactis INIA 415-2, a spontaneous mutant not producing bacteriocins. Cheeses made individually with the lactococcal strains, without clostridial spores, served as controls. Cheese made with clostridial spores and L. lactis subsp. lactis INIA 415-2 showed late blowing defect after 120days of ripening. Spoilt cheese also showed lower concentrations of lactic acid, and higher levels of acetic, propionic and butyric acids, and of other volatile compounds such as 2-propanol and 1-butanol, than control cheese. In addition, cheese made with the bacteriocin producer did not show any late blowing symptoms, despite its spore counts similar to those of blown cheese, pointing to outgrowth inhibition of C. beijerinckii spores by bacteriocins. Besides, cheese made with the bacteriocin producer showed similar concentrations of lactic acid and volatile compounds than control cheese. Inclusion of L. lactis subsp. lactis INIA 415 in starter cultures seems a feasible method to prevent late blowing defect in cheese without altering its sensory characteristics.

  11. Isolation and characterization of an efficient bacterial cellulose producer strain in agitated culture: Gluconacetobacter hansenii P2A.

    Aydın, Yasar Andelib; Aksoy, Nuran Deveci


    In this study, typical niches of acetic acid bacteria were screened for isolation of cellulose producer strains. Hestrin Schramm broth was used as enrichment and production media. Only nine out of 329 isolates formed thick biofilms on liquid surface and were identified as potential cellulose producers. Physiological and biochemical tests proved that all cellulose producers belonged to Gluconacetobacter genus. Most productive and mutation-resistant strain was subjected to 16S rRNA sequence analysis and identified as Gluconacetobacter hansenii P2A due to 99.8 % sequence similarity. X-ray diffraction analysis proved that the biofilm conformed to Cellulose I crystal structure, rich in Iα mass fraction. Static cultivation of G. hansenii P2A in HS medium resulted with 1.89 ± 0.08 g/l of bacterial cellulose production corresponding to 12.0 ± 0.3 % yield in terms of substrate consumption. Shaking and agitation at 120 rpm aided in enhancement of the amount and yield of produced cellulose. Productivity and yield reached up to 3.25 ± 0.11 g/l and 17.20 ± 0.14 % in agitated culture while a slight decrease from 78.7 % to 77.3 % was observed in the crystallinity index.

  12. The use of Macronet resins to recover gamma-decalactone produced by Rhodotorula aurantiaca from the culture broth.

    Alchihab, Mohamed; Aldric, Jean-Marc; Aguedo, Mario; Destain, Jacqueline; Wathelet, Jean-Paul; Thonart, Philippe


    During the biotransformation of castor oil into gamma-decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of gamma-decalactone of 6.5 g/l was obtained. The parameters of gamma-decalactone adsorption on three Macronet resins (MN-202, MN-102 and MN-100) were investigated in water. Adsorption isotherms of gamma-decalactone for the three Macronet resins were linear. The trapping of gamma-decalactone produced by R. aurantiaca on these resins was then carried out. gamma-Decalactone was effectively retained by all the studied Macronet resins. The resin MN-202 trapped gamma-decalactone more efficiently than MN-102 and MN-100. The percentages of gamma-decalactone adsorbed on the resins MN-202, MN-102 and MN-100 were, respectively, 85, 75 and 81%, whereas around 70% of the adsorbed gamma-decalactone was then desorbed. We propose an industrial process that uses Macronet resins to extract gamma-decalactone from culture broth of R. aurantiaca.

  13. Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus.

    Melo, André L A; Soccol, Carlos R; Thomaz-Soccol, Vanete; Nogueira, Miodeli


    Bioinsecticides are shown to be useful in control programs to prevent several diseases, based on their specificity and efficiency against insect vectors. In the current study a bioinsecticide based on Bacillus sphaericus was produced using a white soybean culture medium and applied to larvae of Culex quinquefasciatus, the susceptible species, and Aedes aegypti, the refractory species used as the negative control. Efficacy was compared with that of the product fermented with the Luria Bertani (LB) reference medium. The experiments showed that C. quinquefasciatus was highly susceptible to the product prepared with white soybean meal, reaching 100% larval mortality even at 10mg/L, while A. aegypti failed to reach 70% mortality at a concentration of 1g/L. By comparison with the reference medium, the proposed culture medium showed high larvicidal power, reaching a LD90 of 2.26 mg/L, while 4.37 mg/L was needed for the LB medium to achieve the same mortality rate. Cost comparison between the formulations favored the use of the bioinsecticide produced with white soybean meal. After factoring in the LD90 value, the cost ratio favored the new raw material by nearly 1:220.

  14. Characterization of an immunomodulatory Der p 2-FIP-fve fusion protein produced in transformed rice suspension cell culture.

    Su, Chin-Fen; Kuo, I-Chun; Chen, Peng-Wen; Huang, Chiung-Hui; Seow, See Voon; Chua, Kaw Yan; Yu, Su-May


    Der p 2, a major allergen of Dermatophagoides pteronyssinus mites, is one of the most clinically relevant allergens to allergic patients worldwide. FIP-fve protein (Fve) from the golden needle mushroom (Flammulina velutipes) is an immunomodulatory protein with potential Th1-skewed adjuvant properties. Here, we produced and immunologically evaluated a Der p 2-Fve fusion protein as a potential immunotherapeutic for allergic diseases. Using an inducible expression system in cultured rice suspension cells, the recombinant Der p 2-Fve fusion protein (designated as OsDp2Fve) was expressed in rice cells under the control of an α-amylase gene (αAmy8) promoter and secreted under sucrose starvation. OsDp2Fve was partially purified from the cultured medium. The conformation of Der p 2 in OsDp2Fve remains intact as reflected by its unaltered allergenicity, as assessed by human IgE ELISA and histamine release assays, compared to non-fusion Der p 2 protein. Furthermore, the Fve protein expressed in OsDp2Fve retains its in vitro lymphoproliferative activity but loses its hemagglutination and lymphoagglutination effects compared to the native protein. Notably, in vivo evaluation showed that mice administered with OsDp2Fve possessed an enhanced production of Der p 2-specific IgG antibodies without potentiating the production of Der p 2-specific IgE and Th2 effector cytokines in comparison with mice co-administered with native Fve and Der p 2 proteins. These results suggest that the recombinant Der p 2-Fve fusion protein produced in rice suspension cell cultures has a great potential for allergy immunotherapy.

  15. Effect of different in vitro culture extracts of black pepper (Piper nigrum L.) on toxic metabolites-producing strains.

    Ahmad, Nisar; Abbasi, Bilal Haider; Fazal, Hina


    In the present study, the effect of different in vitro cultures (callus, in vitro shoots) and commercially available peppercorn extract was investigated for its activity against toxic metabolite-producing strains (Escherichia coli, Pseudomonas aeroginosa, Salmonella typhi, Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, and Candida albicans). These in vitro cultures were extracted with ethanol, hexane, and chloroform, and the antipathogenic activity was determined by well-diffusion method. Hexane extract of callus showed 22 mm zone of inhibition against B. cereus, 23 mm against S. aureus, while regenerated shoots and seeds have shown 24.3 and 26 mm zones of inhibition. The ethanolic extracts of regenerated Piper shoots have shown 25 mm activity against S. aureus, 21 mm against B. cereus, and 16 mm in the case of C. albicans in comparison with standard antibiotics. Peppercorn extracts in chloroform and ethanol had shown activities against B. cereus (23.6 mm) and B. subtilis (23.5 mm). During in vitro organogenesis and morphogenesis, cells and tissues produced a comparable phytochemicals profile like mother plant. Morphogenesis is critically controlled by the application of exogenous plant-growth regulators. Such addition alters the hormonal transduction pathways, and cells under in vitro conditions regenerate tissues, which are dependant on the physiological state of cells, and finally enhance the production of secondary metabolites. To the best of our knowledge, this is the first report to compare the antimicrobial potential of in vitro regenerated tissues and peppercorn with standard antibiotics. In conclusion, most of the extracts showed pronounced activities against all the pathogenic microbes. This is a preliminary work, and the minimum inhibitory concentration values needs to be further explored. Regenerated tissues of P. nigrum are a good source of biologically active metabolites for antimicrobial activities, and callus culture presented itself as

  16. Antimicrobial peptides (AMPs) produced by Saccharomyces cerevisiae induce alterations in the intracellular pH, membrane permeability and culturability of Hanseniaspora guilliermondii cells.

    Branco, Patrícia; Viana, Tiago; Albergaria, Helena; Arneborg, Nils


    Saccharomyces cerevisiae produces antimicrobial peptides (AMPs) during alcoholic fermentation that are active against several wine-related yeasts (e.g. Hanseniaspora guilliermondii) and bacteria (e.g. Oenococcus oeni). In the present study, the physiological changes induced by those AMPs on sensitive H. guilliermondii cells were evaluated in terms of intracellular pH (pHi), membrane permeability and culturability. Membrane permeability was evaluated by staining cells with propidium iodide (PI), pHi was determined by a fluorescence ratio imaging microscopy (FRIM) technique and culturability by a classical plating method. Results showed that the average pHi of H. guilliermondii cells dropped from 6.5 (healthy cells) to 5.4 (damaged cells) after 20 min of exposure to inhibitory concentrations of AMPs, and after 24 h 77.0% of the cells completely lost their pH gradient (∆pH=pHi-pHext). After 24h of exposure to AMPs, PI-stained (dead) cells increased from 0% to 77.7% and the number of viable cells fell from 1×10(5) to 10 CFU/ml. This means that virtually all cells (99.99%) became unculturable but that a sub-population of 22.3% of the cells remained viable (as determined by PI staining). Besides, pHi results showed that after 24h, 23% of the AMP-treated cells were sub-lethally injured (with 0pH<3). Taken together, these results indicated that this subpopulation was under a viable but non-culturable (VBNC) state, which was further confirmed by recuperation assays. In summary, our study reveals that these AMPs compromise the plasma membrane integrity (and possibly also the vacuole membrane) of H. guilliermondii cells, disturbing the pHi homeostasis and inducing a loss of culturability. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. H-ras expression in immortalized keratinocytes produces an invasive epithelium in cultured skin equivalents.

    Melville B Vaughan

    Full Text Available BACKGROUND: Ras proteins affect both proliferation and expression of collagen-degrading enzymes, two important processes in cancer progression. Normal skin architecture is dependent both on the coordinated proliferation and stratification of keratinocytes, as well as the maintenance of a collagen-rich basement membrane. In the present studies we sought to determine whether expression of H-ras in skin keratinocytes would affect these parameters during the establishment and maintenance of an in vitro skin equivalent. METHODOLOGY/PRINCIPAL FINDINGS: Previously described cdk4 and hTERT immortalized foreskin keratinocytes were engineered to express ectopically introduced H-ras. Skin equivalents, composed of normal fibroblast-contracted collagen gels overlaid with keratinocytes (immortal or immortal expressing H-ras, were prepared and incubated for 3 weeks. Harvested tissues were processed and sectioned for histology and antibody staining. Antigens specific to differentiation (involucrin, keratin-14, p63, basement-membrane formation (collagen IV, laminin-5, and epithelial to mesenchymal transition (EMT; e-cadherin, vimentin were studied. Results showed that H-ras keratinocytes produced an invasive, disorganized epithelium most apparent in the lower strata while immortalized keratinocytes fully stratified without invasive properties. The superficial strata retained morphologically normal characteristics. Vimentin and p63 co-localization increased with H-ras overexpression, similar to basal wound-healing keratinocytes. In contrast, the cdk4 and hTERT immortalized keratinocytes differentiated similarly to normal unimmortalized keratinocytes. CONCLUSIONS/SIGNIFICANCE: The use of isogenic derivatives of stable immortalized keratinocytes with specified genetic alterations may be helpful in developing more robust in vitro models of cancer progression.

  18. Culturing-based Temperature Calibration of a Genetically Distinct, Alkenone-producing Haptophyte Species isolated from Lake George, ND

    Zheng, Y.; Andersen, R. A.; Huang, Y.; Amaral-Zettler, L. A.


    Lacustrine alkenones are rapidly becoming an important tool for continental paleoclimate reconstructions. However, DNA sequencing of 18S ribosomal RNA marker genes has uncovered multiple species of haptophytes in different lakes. To date, there are only two isolated lacustrine species Chrysotila lamellosa and Isochrysis galbana available for culture studies. In our study, we report the isolation of a new haptophyte species from Lake George (LG) that, based on analyses of partial large subunit rRNA gene sequences, is genetically distinct from both Chrysotila lamellosa and Isochrysis galbana. We examined alkenone unsaturation index UK37 values for the LG species at 4°C, 10°C, 15°C, 20°C and 25°C as a function of temperature in a culture experiment. The temperature sensitivity of the new species was significantly higher than previously cultured Isochrysis galbana and Chrysotila lamellosa strains, with a slope that was 25 to 100 % higher. We found that the best linear relationship was obtained when two double-bond alkenones were excluded from the calibration (we developed an index termed UK''37 = [C37:4] / [C37:3+C37:4]). In particular, UK''37 is more linear to the growth temperature than UK37 at low (4-10°C) and high (20-25°C) temperature ranges. Our experiments show that both UK37 and UK''37 of this new alkenone-produced species is strongly controlled by culture temperature and can be used for paleoclimate reconstruction. However, we recommend the use of UK''37 index to reconstruct temperature if the haptophyte's growing environment falls within temperature extremes (4-10°C and 20-25°C). This newly cultivated species broadens our ability of applying lacustrine haptophyte calibrations to continental paleothermometry.

  19. Efforts to accelerate domestication of winged bean (Psophocarpus tetragonolobus (L.) DC.) by means of induced mutations and tissue culture.

    Klu, G.Y.P.


    This thesis describes mutation breeding and tissue culture techniques developed for accelerated domestication of winged bean ( Psophocarpustetragonolobus (L.) DC.). The tissue culture techniques, which are the first steps towards genetic transformation of the crop, include: (1) direct adventitious s

  20. An intensely sympathetic awareness: experiential similarity and cultural norms as means for gaining older African Americans' trust of scientific research.

    Sabir, Myra G; Pillemer, Karl A


    Well-known trust-building methods are routinely used to recruit and retain older African Americans into scientific research studies, yet the quandary over how to overcome this group's hesitance to participate in research remains. We present two innovative and testable methods for resolving the dilemma around increasing older African Americans' participation in scientific research studies. Certain specific and meaningful experiential similarities between the primary researcher and the participants, as well as clear recognition of the elders' worth and dignity, improved older African Americans' willingness to adhere to a rigorous research design. Steps taken in an intervention study produced a potentially replicable strategy for achieving strong results in recruitment, retention and engagement of this population over three waves of assessment. Sixty-two (n=62) older African Americans were randomized to treatment and control conditions of a reminiscence intervention. Sensitivity to an African American cultural form of respect for elders (recognition of worth and dignity), and intersections between the lived experience of the researcher and participants helped dispel this population's well-documented distrust of scientific research. Results suggest that intentional efforts to honor the worth and dignity of elders through high level hospitality and highlighting meaningful experiential similarities between the researcher and the participants can improve recruitment and retention results. Experiential similarities, in particular, may prove more useful to recruitment and retention than structural similarities such as age, race, or gender, which may not in themselves result in the trust experiential similarities elicit.

  1. Anti-tumor Effects of Exo- and Endo-biopolymers Produced from Submerged Cultures of Three Different Mushrooms

    Jeong, Yong-Tae; Yang, Byung-Keun; Li, Chun-ru


    The anti-tumor effects of exo- (EX) and endo-biopolymers (EN) produced from submerged mycelial cultures of Ganoderma applanatum (GA), Collybia confluens (CC), and Pleurotus eryngii (PE) were studied using Sarcoma 180 bearing mice. Solid tumor growth was inhibited most effectively when 40 mg/kg body weight (BW) of GA-EX or PE-EN was administered to the intraperitoneal (i.p.) cavity of BALB/c mice. The spleen and liver indexes were increased in mice following i.p. administration of GA-EX and PE-EN fractions. GA-EX and PE-EN reduced the tumor formation by 30.7% and 29.4%, respectively. GA-EX and PE-EN increased the natural killer (NK) cell activity of splenocytes by 41.3% and 28.9%, respectively. PMID:23990743

  2. Stochastic modelling of Listeria monocytogenes single cell growth in cottage cheese with mesophilic lactic acid bacteria from aroma producing cultures.

    Østergaard, Nina Bjerre; Christiansen, Lasse Engbo; Dalgaard, Paw


    A stochastic model was developed for simultaneous growth of low numbers of Listeria monocytogenes and populations of lactic acid bacteria from the aroma producing cultures applied in cottage cheese. During more than two years, different batches of cottage cheese with aroma culture were analysed for pH, lactic acid concentration and initial concentration of lactic acid bacteria. These data and bootstrap sampling were used to represent product variability in the stochastic model. Lag time data were estimated from observed growth data (lactic acid bacteria) and from literature on L. monocytogenes single cells. These lag time data were expressed as relative lag times and included in growth models. A stochastic model was developed from an existing deterministic growth model including the effect of five environmental factors and inter-bacterial interaction [Østergaard, N.B, Eklöw, A and Dalgaard, P. 2014. Modelling the effect of lactic acid bacteria from starter- and aroma culture on growth of Listeria monocytogenes in cottage cheese. International Journal of Food Microbiology. 188, 15-25]. Growth of L. monocytogenes single cells, using lag time distributions corresponding to three different stress levels, was simulated. The simulated growth was subsequently compared to growth of low concentrations (0.4-1.0 CFU/g) of L. monocytogenes in cottage cheese, exposed to similar stresses, and in general a good agreement was observed. In addition, growth simulations were performed using population relative lag time distributions for L. monocytogenes as reported in literature. Comparably good predictions were obtained as for the simulations performed using lag time data for individual cells of L. monocytogenes. Therefore, when lag time data for individual cells are not available, it was suggested that relative lag time distributions for L. monocytogenes can be used as a qualified default assumption when simulating growth of low concentrations of L. monocytogenes.

  3. Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata.

    Kakimoto, Masayuki; Ishikawa, Toshiki; Miyagi, Atsuko; Saito, Kazuaki; Miyazaki, Motonobu; Asaeda, Takashi; Yamaguchi, Masatoshi; Uchimiya, Hirofumi; Kawai-Yamada, Maki


    A volatile metabolite, 2-methylisoborneol (2-MIB), causes an unpleasant taste and odor in tap water. Some filamentous cyanobacteria produce 2-MIB via a two-step biosynthetic pathway: methylation of geranyl diphosphate (GPP) by methyl transferase (GPPMT), followed by the cyclization of methyl-GPP by monoterpene cyclase (MIBS). We isolated the genes encoding GPPMT and MIBS from Pseudanabaena galeata, a filamentous cyanobacterium known to be a major causal organism of 2-MIB production in Japanese lakes. The predicted amino acid sequence showed high similarity with that of Pseudanabaena limnetica (96% identity in GPPMT and 97% identity in MIBS). P. galeata was cultured at different temperatures to examine the effect of growth conditions on the production of 2-MIB and major metabolites. Gas chromatograph-mass spectrometry (GC-MS) measurements showed higher accumulation of 2-MIB at 30 °C than at 4 °C or 20 °C after 24 h of culture. Real-time-RT PCR analysis showed that the expression levels of the genes encoding GPPMT and MIBS decreased at 4 °C and increased at 30 °C, compared with at 20 °C. Furthermore, metabolite analysis showed dramatic changes in primary metabolite concentrations in cyanobacteria grown at different temperatures. The data indicate that changes in carbon flow in the TCA cycle affect 2-MIB biosynthesis at higher temperatures. Copyright © 2013 Elsevier GmbH. All rights reserved.

  4. Evaluating Nitrogen-Containing Biosynthetic Products Produced by Saltwater Culturing of Several California Littoral Zone Gram-Negative Bacteria.

    Lorig-Roach, Nicholas; Still, Patrick C; Coppage, David; Compton, Jennifer E; Crews, Mitchell S; Navarro, Gabriel; Tenney, Karen; Crews, Phillip


    The biosynthetic potential of marine-sediment-derived Gram-negative bacteria is poorly understood. Sampling of California near-shore marine environments afforded isolation of numerous Gram-negative bacteria in the Proteobacteria and Bacteriodetes phyla, which were grown in the laboratory to provide extracts whose metabolites were identified by comparative analyses of LC-mass spectrometry and MS(n) data. Overall, we developed an assemblage of seven bacterial strains grown in five different media types designed to coax out unique secondary metabolite production as a function of varying culture conditions. The changes in metabolite production patterns were tracked using the GNPS MS(2) fragmentation pattern analysis tool. A variety of nitrogen-rich metabolites were visualized from the different strains grown in different media, and strikingly, all of the strains examined produced the same new, proton-atom-deficient compound, 1-methyl-4-methylthio-β-carboline (1), C13H12N2S. Scale-up liquid culture of Achromobacter spanius (order: Burkholderiales; class: Betaproteobacteria) provided material for the final structure elucidation. The methods successfully combined in this work should stimulate future studies of molecules from marine-derived Gram-negative bacteria.

  5. Supplementation of culture medium with L-carnitine improves development and cryotolerance of bovine embryos produced in vitro.

    Takahashi, Toshikiyo; Inaba, Yasushi; Somfai, Tamas; Kaneda, Masahiro; Geshi, Masaya; Nagai, Takashi; Manabe, Noboru


    High lipid content in embryos is associated with low freezing tolerance. This study assessed the effects of exogenous L-carnitine, an enhancer of lipid metabolism, on the in vitro development and freezing survival of bovine embryos. Also, effects on metabolic activity, reactive oxygen species (ROS) and apoptosis were investigated. Supplementation of embryo culture medium with 1.518 mM or 3.030 mM L-carnitine significantly increased the rates of zygote development to the blastocyst stage and blastocyst cell numbers whereas 6.072 mM of this compound did not improve embryo development. Survival rates after slow freezing of blastocysts were significantly higher when embryos were cultured in the presence of 1.518 mM or 3.030 mM L-carnitine compared with the control. A lower density of lipid droplets was detected in L-carnitine-treated blastocysts compared with the control. L-carnitine significantly reduced ROS levels in 2-cell embryos but did not reduce ROS levels at later stages. The apoptotic cell rate was not different between control and L-carnitine-treated blastocysts. L-carnitine significantly increased ATP levels in 2-cell embryos but not at the 8-cell or blastocyst stages. L-carnitine increased the expression of metabolism-related ATP6 and COX1 genes in blastocysts. In conclusion, L-carnitine supplementation enhanced lipid metabolism in embryos resulting in improved development and cryotolerance of bovine blastocysts produced in vitro.

  6. [Colors and their meaning in culture and psychology--a historical outline and contemporary status of color vision theories].

    Grzybowski, Andrzej; Lewicka, Romana; Torlińska, Teresa; Stelcer, Bogusław


    The mechanism of color perception has intrigued scholars from antiquity. However, the understanding of this phenomena only came with the recognition of the nature of light and visual perception. Ancient concepts, present in science until the Renaissance, were based more on philosophical considerations and theoretical speculations than on anatomical studies and a matter-of-fact assessment of physiological functions of the visual system. From antiquity to 17th century scientific approach to the concept of vision was dominated by two theories: intromission and extramission (emanation). Intromission theory, propagated by Alhazen (lbn al.-Haythama), Vitello, John Peckham, Roger Bacon and Leonardo da Vinci, assumed that the light was transmitted from the observed object perpendicularly to the transparent eye structures. Johannes Kepler was the first scholar to propose that the retina was the receptive part of the eye. In the first half of the 17th century, Kepler's groundbreaking optical achievements and anatomical discoveries of many other scientists cast new light on the understanding of the role of different eye structures, finally wiping out the intromission theory. A further major achievement contributing to the recognition of the true nature of colors was a theory presented by Newton in 1688. He argued that they were colored rays, and not white light, that were composed of homogenous and pure light. It was, however, not until the 19th century when two modern theories of color appeared, i.e. a trichromatic theory mostly associated with the names of Young and Hemlholtz, and an opponent colors theory of Hering. In the 20th century, the two theories--previously assumed as contradictory--were joined into the zone theories of color vision. Colors have their cultural and social meanings, as far as a very individual and personal interpretation. In the former function they are used to illustrate some cultural and sociological phenomena; in the latter, they are helpful in

  7. Detection of hydrogen peroxide-producing Lactobacillus species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women

    Balkus Jennifer E


    Full Text Available Abstract Background The presence of hydrogen peroxide (H2O2 producing Lactobacillus in the vagina may play a role in controlling genital HIV-1 shedding. Sensitive molecular methods improve our ability to characterize the vaginal microbiota; however, they cannot characterize phenotype. We assessed the concordance of H2O2-producing Lactobacillus detected by culture with quantitative PCR (qPCR detection of Lactobacillus species commonly assumed to be H2O2-producers. Methods Samples were collected as part of a prospective cohort study of HIV-1 seropositive US women. Cervicovaginal lavage specimens were tested for L. crispatus and L. jensenii using 16S rRNA gene qPCR assays. Vaginal swabs were cultured for Lactobacillus and tested for H2O2-production. We calculated a kappa statistic to assess concordance between culture and qPCR. Results Culture and qPCR results were available for 376 visits from 57 women. Lactobacilli were detected by culture at 308 (82% visits, of which 233 of 308 (76% produced H2O2. L. crispatus and/or L. jensenii were detected at 215 (57% visits. Concordance between detection of L. crispatus and/or L. jensenii by qPCR and H2O2-producing Lactobacillus by culture was 75% (kappa = 0.45. Conclusions Among HIV-1 seropositive women, there was a moderate level of concordance between H2O2-producing Lactobacillus detected by culture and the presence of L. crispatus and/or L. jensenii by qPCR. However, one-quarter of samples with growth of H2O2-producing lactobacilli did not have L. crispatus or L. jensenii detected by qPCR. This discordance may be due to the presence of other H2O2-producing Lactobacillus species.

  8. [Physiological and cell biological characterization of the protease producer Thermoactinomyces vulgaris during prolonged culture in a stirred fermenter].

    Kretschmer, S; Körner, D; Strohbach, G; Klingenberg, P; Jacob, H E; Gumpert, J; Ruttloff, H


    The physiological behaviour of Thermoactinomyces vulgaris - producing a thermostable serine-protease - was analyzed during fermentation. During 38 h the consumption of nutrients and oxygen as well as the rates of macromolecular and protease synthesis were measured. The morphological and ultrastructural changes of the mycelia were also studied. The mycelia grew exponentially for about 5 hours. After a short lag and a second slower growth phase, growth continued about linearly until the end, as was indicated by a constant rate of incorporation of labelled thymidine. However, at the same time a changing portion of hyphae - up to 45% - underwent lysis. According to the changing ratio of growing and lysing material, regarding the physiological activity of the culture the fermentation process could be divided into 4 periods. The formation of the protease started at the transition to the slow growth phase and continued linearly. The ability to produce the protease was attributed to a mycelium being formed after the shift down caused by limitation of supply of utilizable nitrogen compounds. The end of protease production 10 h later was correlated to a drastic decrease of the respiratory activity of the mycelia, probably caused by exhaustion of easily utilizable carbohydrates.

  9. Use of Lactobacillus plantarum LPCO10, a Bacteriocin Producer, as a Starter Culture in Spanish-Style Green Olive Fermentations

    Ruiz-Barba, J. L.; Cathcart, D. P.; Warner, P. J.; Jiménez-Díaz, R.


    Bacteriocin-producing Lactobacillus plantarum LPCO10 and its non-bacteriocin-producing, bacteriocinimmune derivative, L. plantarum 55-1, were evaluated separately for growth and persistence in natural Spanish-style green olive fermentations. Both strains were genetically marked and selectively enumerated using antibiotic-containing media. Plasmid profile and bacteriocin production (bac+) were used as additional markers. When olive brines were inoculated at 105 CFU/ml, the parent strain, LPCO10, proliferated to dominate the epiphytic microflora, sharing high population levels with other spontaneously occurring lactobacilli and persisting throughout the fermentation (12 weeks). In contrast, the derivative strain could not be isolated after 7 weeks. Stability of both plasmid profile and bac+ (LPCO10 strain) or bac- (55-1 strain) phenotype was shown by L. plantarum LPCO10 and L. plantarum 55-1 isolated throughout the fermentation. Bacteriocin activity could be found in the L. plantarum LPCO10-inoculated brines only after ammonium sulfate precipitation and concentration (20 times) of the final brine. Spontaneously occurring lactobacilli and lactic coccus populations, which were isolated from each of the fermenting brines studied during this investigation, were shown to be sensitive to the bacteriocins produced by L. plantarum LPCO10 when tested by the drop diffusion test. The declines in both pH and glucose levels throughout the fermentative process were similar in L. plantarum LPCO10- and in L. plantarum 55-1-inoculated brines and were comparable to the declines in the uninoculated brines. However, the final concentration of lactic acid in L. plantarum LPCO10-inoculated brines was higher than in the L. plantarum 55-1-inoculated brines and uninoculated brines. These results indicated that L. plantarum LPCO10 may be useful as a starter culture to control the lactic acid fermentation of Spanish-style green olives. PMID:16349291

  10. Distinct galactofuranose antigens in the cell wall and culture supernatants as a means to differentiate Fusarium from Aspergillus species.

    Wiedemann, Annegret; Kakoschke, Tamara Katharina; Speth, Cornelia; Rambach, Günter; Ensinger, Christian; Jensen, Henrik Elvang; Ebel, Frank


    Detection of carbohydrate antigens is an important means for diagnosis of invasive fungal infections. For diagnosis of systemic Aspergillus infections, galactomannan is commonly used, the core antigenic structure of which consists of chains of several galactofuranose moieties. In this study, we provide evidence that Fusarium produces at least two distinct galactofuranose antigens: Smaller amounts of galactomannan and larger quantities of a novel antigen recognized by the monoclonal antibody AB135-8. In A. fumigatus, only minor amounts of the AB135-8 antigen are found in supernatants and in the apical regions of hyphae. A galactofuranose-deficient A. fumigatus mutant lacks the AB135-8 antigen, which strongly suggests that galactofuranose is an essential constituent of this antigen. Using a combination of AB135-8 and a galactomannan-specific antibody, we were able to unambiguously differentiate A. fumigatus and Fusarium hyphae in immunohistology. Moreover, since Fusarium releases the AB135-8 antigen, it appears to be a promising target antigen for a serological detection of Fusarium infections. Copyright © 2016 Elsevier GmbH. All rights reserved.

  11. The Meanings of "Culture" in Health and Social Care: A Case Study of the Combined Trust in Somerset.

    Peck, Edward; Towell, David; Gulliver, Pauline


    Evaluated how British health and local authorities integrated mental health services into general health and social services. Stakeholder interviews and surveys showed that the recurrent theme of culture meant different things to different people. Shared culture was widely believed to lead to seamless service and collaboration, but low staff…

  12. Automated detection of micro-organisms in blood cultures by means of the Malthus Microbiological Growth Analyser.

    Brown, D F; Warner, M; Taylor, C E; Warren, R E


    A prototype Malthus Microbiological Growth Analyser was compared with conventional methods for examining blood cultures in a trial of 651 cultures mostly from patients with haematological malignancy or undergoing haemodialysis or renal transplantation. Of 100 significantly positive cultures, organisms from 82 grew in the conventional aerobic (+ CO2) bottle, 78 in the conventional anaerobic bottle and 71 in the Malthus bottle. The differences were not statistically significant (p greater than 0.05). The Malthus system detected 83.6% of significantly positive cultures earlier than the comparable conventional bottles while 7.3% positive cultures were detected earlier by the conventional system. When use of the Malthus system was restricted to the hours of 09.00 to 17.30 daily 27.3% positive cultures were detected earlier by the Malthus system and 16.4% were detected earlier by the conventional system. One of the organisms which grew in the Malthus bottle, a contaminating Staphylococcus epidermidis, was not detected by the Malthus system. Instability of electrodes resulted in 26.9% false positive cultures with the prototype Malthus system. Contamination rates in both the Malthus and conventional anaerobic bottles were lower than in the aerobic bottles.

  13. No Evidence for a Culturable Bacterial Tetrodotoxin Producer in Pleurobranchaea maculata (Gastropoda: Pleurobranchidae and Stylochoplana sp. (Platyhelminthes: Polycladida

    Lauren R. Salvitti


    Full Text Available Tetrodotoxin (TTX is a potent neurotoxin found in the tissues of many taxonomically diverse organisms. Its origin has been the topic of much debate, with suggestions including endogenous production, acquisition through diet, and symbiotic bacterial synthesis. Bacterial production of TTX has been reported in isolates from marine biota, but at lower than expected concentrations. In this study, 102 strains were isolated from Pleurobranchaea maculata (Opisthobranchia and Stylochoplana sp. (Platyhelminthes. Tetrodotoxin production was tested utilizing a recently developed sensitive method to detect the C9 base of TTX via liquid chromatography—mass spectrometry. Bacterial strains were characterized by sequencing a region of the 16S ribosomal RNA gene. To account for the possibility that TTX is produced by a consortium of bacteria, a series of experiments using marine broth spiked with various P. maculata tissues were undertaken. Sixteen unique strains from P. maculata and one from Stylochoplana sp. were isolated, representing eight different genera; Pseudomonadales, Actinomycetales, Oceanospirillales, Thiotrichales, Rhodobacterales, Sphingomonadales, Bacillales, and Vibrionales. Molecular fingerprinting of bacterial communities from broth experiments showed little change over the first four days. No C9 base or TTX was detected in isolates or broth experiments (past day 0, suggesting a culturable microbial source of TTX in P. maculata and Stylochoplana sp. is unlikely.

  14. No evidence for a culturable bacterial tetrodotoxin producer in Pleurobranchaea maculata (Gastropoda: Pleurobranchidae) and Stylochoplana sp. (Platyhelminthes: Polycladida).

    Salvitti, Lauren R; Wood, Susanna A; McNabb, Paul; Cary, Stephen Craig


    Tetrodotoxin (TTX) is a potent neurotoxin found in the tissues of many taxonomically diverse organisms. Its origin has been the topic of much debate, with suggestions including endogenous production, acquisition through diet, and symbiotic bacterial synthesis. Bacterial production of TTX has been reported in isolates from marine biota, but at lower than expected concentrations. In this study, 102 strains were isolated from Pleurobranchaea maculata (Opisthobranchia) and Stylochoplana sp. (Platyhelminthes). Tetrodotoxin production was tested utilizing a recently developed sensitive method to detect the C9 base of TTX via liquid chromatography-mass spectrometry. Bacterial strains were characterized by sequencing a region of the 16S ribosomal RNA gene. To account for the possibility that TTX is produced by a consortium of bacteria, a series of experiments using marine broth spiked with various P. maculata tissues were undertaken. Sixteen unique strains from P. maculata and one from Stylochoplana sp. were isolated, representing eight different genera; Pseudomonadales, Actinomycetales, Oceanospirillales, Thiotrichales, Rhodobacterales, Sphingomonadales, Bacillales, and Vibrionales. Molecular fingerprinting of bacterial communities from broth experiments showed little change over the first four days. No C9 base or TTX was detected in isolates or broth experiments (past day 0), suggesting a culturable microbial source of TTX in P. maculata and Stylochoplana sp. is unlikely.

  15. Culture-centered engagement with delivery of health services: co-constructing meanings of health in the Tzu Chi Foundation through Buddhist philosophy.

    Dillard, Sydney J; Dutta, Mohan; Sun, Wei-San


    The shift in health communication scholarship from the narrow focus on curing to the complexly intertwined spaces of health, illness, healing, and curing attends to the dynamic cultural contexts within which meanings and practices are negotiated, directing scholarship toward alternative spaces of health care delivery. This study utilized the culture-centered approach as a theoretical lens for providing a discursive space for understanding meanings of health constituted in the practices of the Tzu Chi Foundation, an organization that offers biomedical services within the larger philosophical understandings of Buddhism with 10 million members in over 50 different countries. The emerging perspective promotes non-biomedical meanings of health through selfless giving and assistance founded in Buddhist principles, simultaneously seeking purity of the mind, body, and soul holistically. Through the negotiation of the principles driving Buddhist philosophy and the principles that shape biomedical health care delivery, this study seeks to understand the interpretive frames that circulate among foundation staff and care recipients.

  16. Effects of pyridoxine on rat testes by means of Sertoli-germ cell co-culture system in vitro

    Huang Houjin


    Objective To investigate the effects of pyridoxine on rat testis in vitro. Method an in vitro systen of Sertoligem cell co-culture was applied, the toxic effects of pyridoxine at different concentrations an exposed duration were olserved. Results The detachment of germ cells from sertoli cells showed marked dose-response and time response relafionships with the exposure of pyridoxine. Meanwhile, the characteristic of loosing and ratracting skeletun in the Sertoli cells was found. Conclusions The effects induced by pyridoxine in vitro may reflect damage to Sertoli cells, and testicular cells co-culture could be of value for the study of underlying mechanisms of toxic effects of pyridoxine on rat testis.

  17. Detection of human immunodeficiency virus DNA in cultured human glial cells by means of the polymerase chain reaction

    Teglbjærg, Lars Stubbe; Hansen, J-ES; Dalbøge, H;


    This report describes the use of the polymerase chain reaction (PCR) for the detection of viral genomic sequences in latently infected cells. Infection with human immunodeficiency virus in cultures of human glial cells was demonstrated, using nucleic acid amplification followed by dot blot...... hybridization. It was not possible to detect any viral antigen production in the cultures, and attempts to recover virus by highly sensitive coculture techniques were unsuccessful, indicating that the infection was latent. The PCR technique provides a simple approach to the study of viral infection in cases...

  18. Detection of human immunodeficiency virus DNA in cultured human glial cells by means of the polymerase chain reaction

    Teglbjaerg, L L; Hansen, J E; Dalbøge, H


    This report describes the use of the polymerase chain reaction (PCR) for the detection of viral genomic sequences in latently infected cells. Infection with human immunodeficiency virus in cultures of human glial cells was demonstrated, using nucleic acid amplification followed by dot blot...... hybridization. It was not possible to detect any viral antigen production in the cultures, and attempts to recover virus by highly sensitive coculture techniques were unsuccessful, indicating that the infection was latent. The PCR technique provides a simple approach to the study of viral infection in cases...

  19. Understanding Mexicans and Americans: A Mexican-U.S. Communication Lexicon of Images, Meanings, and Cultural Frames of Reference.

    Diaz-Guerrero, Rogelio; And Others

    This "communication lexicon," a new source of information in the field of language and area studies, describes how selected themes such as family, society, work, and entertainment are perceived and understood by members of the Mexican, Colombian, and United States cultures. It identifies broad trends of perceptions and evaluations…

  20. Detection of human immunodeficiency virus DNA in cultured human glial cells by means of the polymerase chain reaction

    Teglbjaerg, L L; Hansen, J E; Dalbøge, H;


    This report describes the use of the polymerase chain reaction (PCR) for the detection of viral genomic sequences in latently infected cells. Infection with human immunodeficiency virus in cultures of human glial cells was demonstrated, using nucleic acid amplification followed by dot blot...

  1. The Britain that You don't Know-the historical and cultural meaning of British place names



    As products of social interactions between people, place namesalways represent parts of history and culture of the geographicalentities for they are created by using specific national languages.Therefore, we should look beyond the names themselves and dig forthe information behind them.

  2. Nutritionally "Empty" but "Full" of Meanings: The Socio-Cultural Significance of Birthday Cakes in Four Early Childhood Settings

    Albon, Deborah


    This article examines the socio-cultural significance of birthday cakes with the purpose of reflecting upon birthday cake practices enacted in four early childhood settings in England. I argue that birthday cakes occupy an ambiguous place in early childhood practice: seen to be both "risky"--a term I problematise--"and"…

  3. TV Dizileri Yoluyla Yeniden Üretilen Tüketim Kültürü / The Re-produced Consumption Culture by TV Series

    Nesrin Kula


    Full Text Available “Tüketme” terimi “tahrip etmek, harcamak, israf etmek, bitirmek” anlamlarını taşımaktadır. Jean Baudrillard ise, tüketimi kodlar ve kurallarla düzenlenmiş bir göstergeler sistemi olarak tanımlamaktadır. Tüketim tarzları kişilerin kimliklerinin birer unsuru haline gelmektedir. Özneler kimliklerini ifade etmek amacıyla metaları kullanmakta ve kişilerin toplum içindeki statüsü tükettikleri ile ölçülür hale gelmektedir. TV dizileri de, bireylere tüketilecek sonsuz nesneler yoluyla oluşturulacak yaşam tarzları ile ilgili bilgiler vermektedir. Çalışmada, seçilen on dizide üretilen anlam dünyası, yeniden üretilen tüketim ideolojisi ve oluşturulan kadın ve erkek rol modelleri çözümlenmektir. The Re-produced Consumption Culture by TV Series The concept of consumption means ‘to destroy, spend, waste or finish something’. Jean Baudrillard describes the consumption as a semiotic system regulated of codes and rules. Consumption styles are nowadays becoming an element of people’s identity. Individuals use the commodities to express their identities and therefore the status of people in the community being measured according to what they consume. Meanwhile many TV series provides information to individuals about lifestyles to be created by infinite items consumed. This study analyzes the mindset created in ten selected TV series and the re-produced ideology of consumption as well as the presented role models for women and men in them.

  4. Impact of functional mix and starter cultures on the sensory properties of permanent sausages produced in industrial conditions

    Kuzelov, Aco; Naseva, Dijana; Taskov, Nako; Saneva, Dusica; Spasova, Dragica; Andronikov, Darko


    The aim of their search is the impact little bit of functional mixed (composed of glukono delta lactate, ascorbic acid and vitamin C) and starter culture (commercial preparation F - SC111 Bactoferm product company Chr Hansen DK). This product contains a mixed culture composed of Lactobacilus sakei and Staphyococus Camosus frozen in dry form. In three varieties of tea and sausage: I - added a simple sugar sucrose which serve as a control sample, II - with the addition of starter culture, III -...

  5. Oral fibroblasts produce more HGF and KGF than skin fibroblasts in response to co-culture with keratinocytes

    Grøn, Birgitte; Stoltze, Kaj; Andersson, Anders


    The production of hepatocyte growth factor (HGF) and keratinocyte growth factor (KGF) in subepithelial fibroblasts from buccal mucosa, periodontal ligament, and skin was determined after co-culture with keratinocytes. The purpose was to detect differences between the fibroblast subpopulations...... that could explain regional variation in epithelial growth and wound healing. Normal human fibroblasts were cultured on polystyrene or maintained in collagen matrix and stimulated with keratinocytes cultured on membranes. The amount of HGF and KGF protein in the culture medium was determined every 24 h for 5...... days by ELISA. When cultured on polystyrene, the constitutive level of KGF and HGF in periodontal fibroblasts was higher than the level in buccal and skin fibroblasts. In the presence of keratinocytes, all three types of fibroblasts in general increased their HGF and KGF production 2-3 times. When...

  6. Travel, emotion and identity: an exploration into the experiences of students in post 16 education for whom studying in English means working in a foreign language and culture

    Underwood, M James


    This paper involves an exploration into the experiences of students in post 16 education taking traditional academic subjects, in the UK, for whom studying in English means working in a foreign language and culture. The students who formed the sample were high achieving students aged sixteen and seventeen from China, Vietnam and Thailand who had come to study in the UK in order to proceed to a British University. In the first part of this paper, the author proposes a conceptual framework suit...

  7. Terminal values and meaning in life among university students with varied levels of altruism in the present period of socio-cultural change

    Głaz Stanisław


    Full Text Available Abstract The author of this paper, interested in the issues of values preference, meaning in life and altruism among university students has attempted to show a relation between them in the present period of clearly noticeable socio-cultural change. The study was conducted in 2009-2010 in Kraków among university students. The age of the respondents ranged from 21 to 25. 200 sets of correctly completed questionnaires were used for the results analysis.

  8. Centralizing context and culture in the co-construction of health: localizing and vocalizing health meanings in rural India.

    Basu, Ambar; Dutta, Mohan J


    A growing number of communication scholars have articulated the need for understanding context as a key component of health meanings. In this project, the authors seek to explore the role of context in the domain of health meanings in tribal India. The tribal population in India comprises people who have been consistently isolated and exploited, and stripped of their rights and resources. Interest in their health is propelled by this marginalization and their existence in the twilight of tradition and modernization. This article, through the use of participant narratives and a grounded theory of analysis, aims to lay out how meanings of health are contextually constructed by tribals in India. The results demonstrate the constant pain and hardship that envelop their lives, their pining for structural capabilities, and a dialectical tension between tradition and modernization in the coexistence of multiple treatment options.

  9. Shaping beside student of the special medical group of the culture sound lifestyle by means of computer technology

    Vovk L.V.


    Full Text Available Considered results of the pedagogical experiment with use computer information-diagnostic system "Passport of health" on shaping the culture sound lifestyle beside student of the special medical group. Two groups student have took part in experiment in amount 151 persons. It is proved that pedagogical experiment has positively influenced upon fortification of health, life activity, high level of the knowledges and practical skills during education in high school. It is installed that one of the main of the conditions to efficiency of the physical education, are a knowledges, which allow effective to solve the delivered problems of the physical education to personalities.

  10. A Cross-Cultural Study on Meaning and the Nature of Children's Experiences in Australian and French Swimming Clubs

    Light, Richard


    This article reports on a study conducted in Australia and France that inquired into the meaning and the nature of children's experiences of being in swimming clubs with a focus on the positive aspects of membership that keep them in their clubs. Three-month long case studies were conducted in a club in Australia and in a club in France, employing…

  11. A Cross-Cultural Study on Meaning and the Nature of Children's Experiences in Australian and French Swimming Clubs

    Light, Richard


    This article reports on a study conducted in Australia and France that inquired into the meaning and the nature of children's experiences of being in swimming clubs with a focus on the positive aspects of membership that keep them in their clubs. Three-month long case studies were conducted in a club in Australia and in a club in France, employing…

  12. Distinct galactofuranose antigens in the cell wall and culture supernatants as a means to differentiate Fusarium from AspergillusspeciesAnnegret

    Wiedemann, Annegret; Kakoschke, Tamara Katharina; Speth, Cornelia


    tDetection of carbohydrate antigens is an important means for diagnosis of invasive fungal infections. Fordiagnosis of systemic Aspergillus infections, galactomannan is commonly used, the core antigenic struc-ture of which consists of chains of several galactofuranose moieties. In this study, we ...

  13. Birth weight and gestation length of Japanese black calves following transfer of embryos produced in vitro with or without co-culture.

    Numabe, T; Oikawa, T; Kikuchi, T; Horiuchi, T


    Birth weight and gestation length of calves following the transfer of in vitro produced (IVP) embryos with or without co-culture of cumulus cells, were compared to those produced in vivo (IVD). Spermatozoa from one Japanese Black bull were used for both IVP and IVD. IVP embryos were produced using two types of culture method: 1) co-culturing with cumulus cells in TCM 199 supplemented with calf serum (IVP-Co), and 2) non-co-culturing without cumulus cells in CR1aa supplemented with BSA / calf serum (IVP-NON-Co). Both IVP and IVD embryos were transferred non-surgically to Holstein recipients on day 7+/-1 of the estrous cycle. Birth weight and gestation length of half-sib single calves were analyzed. No differences were observed in birth weight and gestation length between IVP-Co and IVP-NON-Co calves (31.0 kg and 31.8 kg, and 291.9 days and 291.0 days, respectively). However, the birth weight of the IVP-Co and IVP-NON-Co calves was significantly higher than that of the IVD calves (PGestation length of the IVP-Co and IVP-NON-Co calves was also significantly longer than that of the IVD calves (P<0.01).

  14. Assessing the efficacy of endoscopic office olfactory biopsy sites to produce neural progenitor cell cultures for the study of neuropsychiatric disorders.

    Wrobel, Bozena B; Mazza, Jill M; Evgrafov, Oleg V; Knowles, James A


    The olfactory region is capable of continuous neurogenesis. Situated on the cribriform plate and segments of the superior septum and both superior and middle turbinates, it is accessible through office-based biopsy and can be used to generate neural progenitor cells to study molecular abnormalities associated with neuropsychiatric disorders. The purpose of the study was to evaluate the efficacy of the endoscopic office olfactory biopsy from middle turbinate and superior-posterior septum to produce the neural progenitor cells. Endoscopic office-based biopsy samples were collected and cultured neuronal cells derived from olfactory neuroepithelium (CNON) were established from 40 healthy individuals and 40 schizophrenia patients. All patients underwent biopsies of both the middle turbinate and the superior-posterior septum. Specific culture conditions promoted the growth of neural progenitor cells from these biopsy sites. CNON cultures were established from such outgrowing neuronal cells. The study was institutional review board (IRB)-approved and informed consent was obtained. Cultures were successfully developed from 98.8% of participants. No complications were observed. The single, unsuccessful specimen failed to grow any cell types due to tissue mishandling. Overall, we have observed no significant difference in the effectiveness of biopsy from middle turbinate and superior-posterior septum to produce neural progenitor cells. The middle turbinate biopsies contain viable neural progenitor cells capable of generating neuronal cell cultures. Thus technically more simple biopsy of the middle turbinate can be used to propagate neural progenitor cells. © 2013 ARS-AAOA, LLC.

  15. Rapid detection of enterobacteriaceae producing extended spectrum beta-lactamases directly from positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

    Oviaño, M; Fernández, B; Fernández, A; Barba, M J; Mouriño, C; Bou, G


    Bacteria that produce extended-spectrum β-lactamases (ESBLs) are an increasing healthcare problem and their rapid detection is a challenge that must be overcome in order to optimize antimicrobial treatment and patient care. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been used to determine resistance to β-lactams, including carbapenems in Enterobacteriaceae, but the methodology has not been fully validated as it remains time-consuming. We aimed to assess whether MALDI-TOF can be used to detect ESBL-producing Enterobacteriaceae from positive blood culture bottles in clinical practice. In the assay, 141 blood cultures were tested, 13 of them were real bacteraemias and 128 corresponded to blood culture bottles seeded with bacterial clinical isolates. Bacteraemias were analysed by MALDI-TOF after a positive growth result and the 128 remaining blood cultures 24 h after the bacterial seeding. β-lactamase activity was determined through the profile of peaks associated with the antibiotics cefotaxime and ceftazidime and its hydrolyzed forms. Clavulanic acid was added to rule out the presence of non-ESBL mechanisms. Overall data show a 99% (103 out of 104) sensitivity in detecting ESBL in positive blood cultures. Data were obtained in 90 min (maximum 150 min). The proposed methodology has a great impact on the early detection of ESBL-producing Enterobacteriaceae from positive blood cultures, being a rapid and efficient method and allowing early administration of an appropriate antibiotic therapy.

  16. Cultural Alignment and Game-Based Learning

    Hammer, Jessica; Davidson, Drew


    Culture can be understood as systems that produce and reproduce meaning, or as stable sets of meanings within a community. The authors illustrate how educational games produce and reproduce meaning, both related to the content being addressed and about the educational process itself. Educational games also rely on shared meanings and norms, which…

  17. [Cultural meanings and relations to the world in psychopathological disorders during pregnancy and the puerperium in the Cameroonian context].

    Mboua, C P; Mboua, N H Olga


    Pregnancy, delivery, and the postpartum period are significant moments that raise questions about women's relation to the world. Globally, the puerperium (the first 42 days after delivery) remains a time when several psychopathological disorders, related to the sexual sphere, may be manifested. This article considers some standard psychopathological knowledge that sheds light on this, while stressing the impact of culture on the woman's individuality and her psychosexual life experiences. It underlines the sensitizing role of psychological experience, affected by beliefs and various taboos, that can cause her to relive traumas from her psychosexual life experience; the women is often unaware of these experiences, but they make it possible to interpret and prevent disorders during the puerperium.

  18. Women in management means women in power: implications for society, family and culture in the Arab world.

    King-irani, L


    A May 1995 conference in Beirut on "The Arab Woman and Business Management" focused on the fact that women are relatively absent from decision-making positions in the public and private sectors in the Arab world. Conference participants also identified characteristics of Arab women who have successfully penetrated the ranks of management, discussed how to balance work and home life, and considered the potentially important role of women managers in the nonprofit sector. Recurring and interrelated themes of the conference addressed the fact that women who have become empowered at work will have increased self-confidence in every aspect of their lives. Also, women who have successfully managed a busy household are able to transfer these skills to the workplace. Women will increasingly demand access to the highest ranks of decision-making, but their culturally-shaped sense of themselves may ultimately hinder them from attaining positions currently reserved for men. Research has shown that women's conceptions of power are more egalitarian and cooperative than the competitiveness exhibited by men. Women may, therefore, offer an important approach to the rapidly changing world market which requires the cooperation of people from diverse backgrounds. Ultimately, it is women who define themselves (rather than accept an identity chosen by society, the family, or culture) who will progress the fastest and achieve the highest ranks in management. Women should neither passively wait to be handed power not allow men to define the nature and uses of power. Instead they must seize their own power and exercise it in their own ways.

  19. [Development of a gentamicin producer under different culture conditions studied by a method of differential centrifugation of the mycelium in a saccharose density gradient].

    Losev, V V; Laznikova, T N; Dmitrieva, S V


    The method of differential centrifugation in the sucrose density gradient (SDG) enabled one to trace the changes in the development of the seed and fermentation mycelium of the gentamicin-producing organism. Correlation between gentamicin distribution in the SDG and the culture productivity was found. It was shown that the culture grown under the optimal aeration and agitation conditions was characterized by formation of higher amounts of the mycelium in the 5th and 6th layers of the SDG. Such mycelium was more productive than that from the other SDG layers. The most productive 48-hour seed culture had the more significant part of the mycelium in the 3rd layer of the SDG. When such a culture had the more significant part of the mycelium in the 3rd layer of the SDG. When such a culture was used as the seed material, the activity of the fermentation broth was the highest. The method of differential centrifugation in the SDG provides determination of the culture productivity by the volumes of the fermentation mycelium in the 5th and 6th layers or the seed mycelium in the 3rd layer of the SDG.

  20. Prevalence, mean intensity and site preference of Caligus rotundigenitalis Yü, 1933 (Copepoda: Caligidae) on cage cultured crimson snapper (Lutjanus erythropterus Bloch, 1790) from Bukit Tambun, Penang, Malaysia.

    Leaw, Yoon Yau; Faizah, Shaharom; Anil, Chatterji; Kua, Beng Chu


    Snapper had been cultured in Malaysia since 1980 due to the fry availability and the high demand. However, details on the caligids infestation were not properly documented. This study was carried out to determine the prevalence, mean intensity and site preference of Caligus rotundigenitalis (Caligidae, Siphonostomatoida) a parasitic copepod on cage cultured crimson snapper, Lutjanus erythropterus from Bukit Tambun, Penang, Malaysia. A total of 70 specimens of cultured snapper were examined based on different infestation sites such as head, body as well as operculum. The specimens were separated into three groups according to the size of the fish. C. rotundigenitalis was found to be the only species infesting L. erythropterus with the prevalence and the mean intensity of 81.4% and 5.6±4.4, respectively. There was a significant difference between the prevalence of site infestation of the body and inner operculum sites. The prevalence of C. rotundigenitalis was highest on inner operculum of the fish followed by the body and head. However, there was no significant difference in the distribution of C. rotundigenitalis over the different infestation sites derived from the three groups. The information obtained from this study can be used for more effective control measures of ectoparasitic copepod infestation in floating cages.

  1. Analysis of the exopolysaccharides produced by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772 grown in continuous culture on glucose and fructose.

    Grobben, G.J.; Casteren, van W.H.M.; Schols, H.A.; Oosterveld, A.; Sala, G.; Smith, M.R.; Sikkema, J.; Bont, de J.A.M.


    The exopolysaccharides produced by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772 grown in defined medium were investigated. At equal cell densities, the strain produced 95 mg l−1 exopolysaccharides with glucose and 30 mg l−1 with fructose as the carbohydrate source. High-performance size-exc

  2. Classes in physical culture and sport as a means of prevention of harmful habits of the student youth

    Shlyapnikova, A. V.; Churina, E. D.; Mishneva, S. D.; Simonova, I. M.; Шляпникова, А. В.; Чурина, Е. Д.; Мишнева, С. Д.; Симонова, И. М.


    The article provides a theoretical and methodological materials on the impact of physical culture and sports the prevention of asocial manifestations, drug abuse and crime among young people Статья содержит теоретические и методические материалы о влиянии фи-зической культуры и спорта на профилактику асоциальных проявлений, наркоманию и преступность среди молодежи....

  3. Feasibility study of an alkaline-based chemical treatment for the purification of polyhydroxybutyrate produced by a mixed enriched culture

    Jiang, Y.; Mikova, G.; Kleerebezem, R.; Van der Wielen, L.A.M.; Cuellar Soares, M.C.


    This study focused on investigating the feasibility of purifying polyhydroxybutyrate (PHB) from mixed culture biomass by alkaline-based chemical treatment. The PHB-containing biomass was enriched on acetate under non-sterile conditions. Alkaline treatment (0.2 M NaOH) together with surfactant SDS (0

  4. Nondairy beverage produced by controlled fermentation with potential probiotic starter cultures of lactic acid bacteria and yeast.

    Freire, Ana Luiza; Ramos, Cintia Lacerda; da Costa Souza, Patrícia Nirlane; Cardoso, Mauro Guilherme Barros; Schwan, Rosane Freitas


    This work aimed to develop a nondairy fermented beverage from a blend of cassava and rice based on Brazilian indigenous beverage cauim using probiotic lactic acid bacteria (LAB) and yeast. The indigenous strains Lactobacillus plantarum CCMA 0743 (from cauim) and Torulaspora delbrueckii CCMA 0235 (from tarubá), and the commercial probiotic, L. acidophilus LAC-04, were used as starter cultures in single and co-cultivations. The bacteria populations were around 8.0 log (CFU/mL) at the end of all fermentations as recommended for probiotic products. Higher residual starch contents were noted in the single LAB cultures (10.6% [w/w]) than in co-cultures (1.6g/L) and ethanol was lower than 0.5% (w/v) consisting in a non-alcoholic beverage. The assays containing yeast showed the highest antioxidant activity (around 10% by DPPH and ABTS methods). Therefore, a nondairy fermented beverage was successfully obtained, and the co-culture of LAB and T. delbrueckii could increase the product's functional properties.

  5. Producing biodiesel from cotton seed oil using Rhizopus oryzae ATTC #34612 whole cell biocatalysts: Culture media and cultivation period optimization

    The effect of culture medium composition and cultivation time on biodiesel production by Rhizopus oryzae ATCC #34612 whole cell catalysts, immobilized on novel rigid polyethylene biomass supports, was investigated. Supplementation of the medium with carbon sources led to higher lipase activity and i...

  6. A comparative analysis of the influence of economic culture on East and West German consumers' subjective product meaning

    Grunert, Klaus G.; Grunert, Suzanne C.


    Executive summary: 1. Consumers in central planning economies have developed different skills from consumers in market economies: while the former have developed skills in locating products, the latter have developed skills in comparing and deciding between products. Even now, some...... of products is the same as in the West, and there are considerable price differences. 3. Differences between East and West German consumers were analysed by comparing samples of consumers in Kiel and Rostock. It was analysed how they perceive food products (frozen ready meals and cheese), and how they relate...... them to values (means-end approach). 4. While product perceptions are more focussed in the West and more fuzzy in the East, the basic perceptual dimensions are the same: they are based on concrete product attributes. 5. An analysis of the fundamental life values of East and West German consumers (using...

  7. Characterization and Clinical Implication of Th1/Th2/Th17 Cytokines Produced from Three-Dimensionally Cultured Tumor Tissues Resected from Breast Cancer Patients

    Anna Kiyomi


    Full Text Available OBJECTIVES: Several cytokines secreted from breast cancer tissues are suggested to be related to disease prognosis. We examined Th1/Th2/Th17 cytokines produced from three-dimensionally cultured breast cancer tissues and related them with patient clinical profiles. METHODS: 21 tumor tissues and 9 normal tissues surgically resected from breast cancer patients were cultured in thermoreversible gelatin polymer–containing medium. Tissue growth and Th1/Th2/Th17 cytokine concentrations in the culture medium were analyzed and were related with hormone receptor expressions and patient clinical profiles. RESULTS: IL-6 and IL-10 were expressed highly in culture medium of both cancer and normal tissues. However, IFN-γ, TNF-α, IL-2, and IL-17A were not detected in the supernatant of the three-dimensionally cultured normal mammary gland and are seemed to be specific to breast cancer tissues. The growth abilities of hormone receptor–negative cancer tissues were significantly higher than those of receptor-positive tissues (P = 0.0383. Cancer tissues of stage ≥IIB patients expressed significantly higher TNF-α levels as compared with those of patients with stage culture system and produce Th1/Th2/Th17 cytokines. Hormone receptor–positive cancer tissues showed less growth ability. TNF-α is suggested to be a biomarker for the cancer stage.

  8. Branding identity with Apple`s iPod : constructing meaning and identity in a consumption culture by using technological equipment

    Blichfeldt, Mathilde F.


    SYNOPSIS This thesis shows how Apple’s mp3 player iPod can serve as an example for how people construct meaning and identity through the use of particular objects while living and participating in a consumption culture. The empirical examples are derived from complex and contextual analysis of iPod; I have performed and analysed four qualitative interviews, various Internet pages, and advertisements for the gadget. In addition, I have in general been observant on how this phenomenon has b...

  9. Optimization of cooperation modern public child-youth organizations and schools in the national-patriotic education by means of physical culture.

    Mischenko O.O.


    Full Text Available It is shown the problem of interaction between school and community organizations in the modern conditions of development of civil society. We consider the optimization of content and institutional framework of the interaction of public associations, and schools by means of physical culture, and also provide information about the place the team in the national-patriotic education of teenagers. The experiment involved children from schools in which the present operation of public children's and youth organizations and activities of these organizations was not detected.

  10. Beneficial effect of two culture systems with small groups of embryos on the development and quality of in vitro-produced bovine embryos.

    Cebrian-Serrano, A; Salvador, I; Silvestre, M A


    Currently, in vitro-produced embryos derived by ovum pick up (OPU) and in vitro fertilization (IVF) technologies represent approximately one-third of the embryos worldwide in cattle. Nevertheless, the culture of small groups of embryos from an individual egg donor is an issue that OPU-IVF laboratories have to face. In this work, we tested whether the development and quality of the preimplantation embryos in vitro cultured in low numbers (five embryos) could be improved by the addition of epidermal growth factor, insulin, transferrin and selenium (EGF-ITS) or by the WOW system. With this aim, immature oocytes recovered from slaughtered heifers were in vitro matured and in vitro fertilized. Presumptive zygotes were then randomly cultured in four culture conditions: one large group (LG) (50 embryos/500 μl medium) and three smaller groups [five embryos/50 μl medium without (control) or with EGF-ITS (EGF-ITS) and five embryos per microwell in the WOW system (WOW)]. Embryos cultured in LG showed a greater ability to develop to blastocyst stage than embryos cultured in smaller groups, while the blastocyst rate of WOW group was significantly higher than in control. The number of cells/blastocyst in LG was higher than control or WOW, whereas the apoptosis rate per blastocyst was lower. On the other hand, the addition of EGF-ITS significantly improved both parameters compared to the control and resulted in similar embryo quality to LG. In conclusion, the WOW system improved embryo development, while the addition of EGF-ITS improved the embryo quality when smaller groups of embryos were cultured.


    Shchekoldina T. V.; Rodionova L. Y.; Chernigovets E. A.


    Healthy nutrition is the foundation of the nation and the most important factor affecting the length of human life. Therefore, the search and the use of new sources of food are important. One of such source is the ancient culture of quinoa (Chenopodium quinoa), known as a "qinoa", "quinva" or "rice quinoa. The uniqueness of the quinoa is its chemical composition, the absence of anti-nutritional substances and unpretentious in growing and harvesting. Currently quinoa is grown in more than 70 c...

  12. Thymus lotocephalus wild plants and in vitro cultures produce different profiles of phenolic compounds with antioxidant activity.

    Costa, Patrícia; Gonçalves, Sandra; Valentão, Patrícia; Andrade, Paula B; Coelho, Natacha; Romano, Anabela


    We compared the phenolic metabolites and antioxidant activities of Thymus lotocephalus G. López & R. Morales wild plants and in vitro cultures using different extraction solvents. HPLC-DAD analysis allowed the identification and quantification of phenolic (caffeic and rosmarinic) acids and flavones (luteolin and apigenin) in extracts from both sources. The in vitro cultures accumulated large amounts of rosmarinic acid. However, extracts from both sources were able to neutralise free radicals in different test systems (TEAC and ORAC assays), to form complexes with Fe(2+) and to protect mouse brains against Fe(2+)-induced lipid peroxidation. The solvent significantly influenced the phenolic content and antioxidant activity of the extracts, water/ethanol being the most efficient for the extraction of antioxidant phytochemicals. We conclude that in vitro cultures of T. lotocephalus represent a promising alternative for the production of valuable natural antioxidants and an efficient tool for the in vitro biosynthesis of rosmarinic acid, therefore avoiding the need to exploit populations of wild plants.

  13. Sensitive method for detecting low numbers of verotoxin-producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin.

    Karmali, M A; Petric, M; Lim, C; Cheung, R; Arbus, G S


    High titers of Verotoxin (VT) were released from cell pellets of VT-producing Escherichia coli (VTEC; corresponding to E. coli strains producing "high" levels of Shiga-like toxin) after incubation in polymyxin B (0.1 mg/ml) for 30 min at 37 degrees C. Maximal titers of polymyxin-releasable VT occurred in cells obtained from 5-h Penassay broth cultures and were up to eightfold higher than the peak culture supernatant VT titers which occurred in 8-h cultures. Polymyxin-releasable cell extracts of 5-h broth cultures inoculated with mixtures of VT-positive (VT+) and VT-negative strains had easily detectable VT titers when the proportion of VT+ cells in the mixture was about 1.0%, but culture supernatants were negative for VT even when this proportion was 20%. The results were the same whether the initial inoculum consisted of broth culture mixtures of VT+ and VT-negative strains or colony sweeps (loopfuls of confluent bacterial growth) taken from solid plate media previously inoculated with the broth mixtures. In a clinical study, 80 stool cultures from patients with hemolytic uremic syndrome and family contacts with diarrhea were tested for free fecal VT, VT in polymyxin extracts of colony sweeps (VT/PECS), and VTEC (examination of 20 separate E. coli colonies from primary media for VT production). Of the 80 samples, 40 were positive for at least one of these three tests; all 40 were positive for free fecal VT, and 20 of these were positive for VT/PECS. VTEC (as few as 1 colony out of 20) were only isolated from 14 of the 20 cultures that were positive for VT/PECS. In six cases, the VT/PECS was positive even when none of 20 colonies tested were VT+, suggesting that the procedure was able to detect a proportion of VTEC that was less than one in 20(5%). We conclude that the VT/PECS method is highly sensitive for detecting low concentrations of VTEC in stools and provides a rapid method for screening out stools that are negative for VTEC. The technique should also be of

  14. Culture, economics, politics and knowledge as meaning-spaces in Social Occupational Therapy: reflections on the experience of “Ponto de Encontro e Cultura”

    Denise Dias Barros


    Full Text Available This article describes and analyzes the “Ponto de Encontro e Cultura - PEC”, an experience developed by METUIA - University of São Paulo (USP-SP in São Paulo from 2007 to 2011 which linked occupational therapy to areas of cultural production. It was attended by the homeless, occupational therapists and students of occupational therapy. To perform the analysis of the different dimensions of the experience we were guided by the meaning-space notion. We took the space as an organizer to understand a reality in which relationships and actions are being empowered in four different spheres: culture, economics, politics and knowledge. We noted that this practice showed that there was an ongoing collective effort to build what may be called piece. This characterizes a process where the space is a common reference point which brings into play different modes of sociability that are created by the management of common symbols and codes. It was important to recognize and appreciate the plurality of modes of knowledge. Thus, we observed that, from the cultural sphere, it is possible to articulate economics, health, social assistance, politics, and knowledge production.

  15. Soft x-ray generation in gases by means of a pulsed electron beam produced in a high-voltage barier discharge

    Azarov, A.V.; Peters, P.J.M.; Boller, K.-J.


    A large area pulsed electron beam is produced by a high-voltage barrier discharge. We compare the properties of the x-rays generated by stopping this beam of electrons in a thin metal foil with those generated by stopping the electrons directly in various gases. The generation of x-rays was investig

  16. 儒家文化中“耻”的心理意蕴及启示%The Psychological Meaning and Revelation of Shame in Confucian Culture



    儒家维护"礼治",提倡"德治",重视"人治",这与个人"耻"感紧密关联。从心理学角度看,"耻"表现为羞愧与内疚的情感体验;"耻"是意识领域中的价值认知与判断,耻的文化生成表现为意识对无意识内容的领悟,个体无意识、集体无意识、文化无意识及社会无意识的共鸣;"耻’感既承载深厚的文化源流,也体现时代特色;儒家"耻"感文化对当今荣辱观教育具有重要的启示作用。%The basic tenets of Confucianism include "the rule of Li(ritual)","the rule of De(virtue)",and "the rule of Ren(humanity)".All bear direct relations to shame as a human emotion.From a psychological perspective,shame represents itself as the experience of embarrassment and guilt.It is value judgment in the consciousness,the conscious understanding of the unconscious.The conscious representation of psychological complexes and archetypes gives rise to internal examination and self-monitoring.The interaction between the conscious and the unconscious completes the internalization of shame.Shame carries deep cultural meaning and varies by cultural norm.In the field of unconsciousness,shame means a sympathy of individual,collective,cultural and social unconsciousness;shame carries both a sense of deep cultural origins,also reflects the characteristics of the times;The culture of Confucian "Chi" inspires us so much on today's moral education.

  17. Cryotolerance of porcine in vitro-produced blastocysts relies on blastocyst stage and length of in vitro culture prior to vitrification.

    Morató, Roser; Castillo-Martín, Míriam; Yeste, Marc; Bonet, Sergi


    The aim of our study was to assess whether the cryotolerance of in vitro-produced embryos could be influenced by the length of in vitro culture and size of blastocoel cavity before vitrification, using the pig as a model. For this purpose we analysed the cryoresistance and apoptosis rate of blastocysts at different stages of development as derived on Day 5 and 6 of in vitro culture. Blastocysts were subsequently vitrified, warmed and cultured for 24h. Re-expansion rates were recorded at 3 and 24h and total cell number and apoptotic cells were determined at 24h. Day-6 blastocysts showed the highest rates of survival after warming, which indicates higher quality compared with Day-5 blastocysts. Higher re-expansion rates were observed for expanded blastocysts and those in the process of hatching when compared with early blastocysts. Total cell number and apoptotic cells were affected by blastocyst stage, vitrification-warming procedures and length of in vitro culture, as expanding and hatching-hatched blastocysts from Day 6 presented higher percentages of apoptotic cells than fresh blastocysts and blastocysts vitrified at Day 5. Our findings suggest that the cryotop vitrification method is useful for the cryopreservation of porcine blastocysts presenting a high degree of expansion, particularly when vitrification is performed after 6 days of in vitro culture. Furthermore, these results show that faster embryo development underlies higher blastocyst cryotolerance and provide evidence that blastocoel cavity expansion before vitrification is a reliable index of in vitro-produced embryo quality and developmental potential.

  18. Differences in the glycosylation profile of a monoclonal antibody produced by hybridomas cultured in serum-supplemented, serum-free or chemically defined media.

    Serrato, J Antonio; Hernández, Vanessa; Estrada-Mondaca, Sandino; Palomares, Laura A; Ramírez, Octavio T


    SFM (serum-free medium) is preferred to media containing animal-derived components when culturing mammalian cells for the production of therapeutic recombinant proteins and mAbs (monoclonal antibodies). Nonetheless, eliminating animal-derived components from media can strongly modify culture performance and alter protein glycosylation. In the present study, mAb glycosylation profiles, extracellular exoglycosidase activities, hybridoma growth and mAb production in traditional medium containing 10% (v/v) FBS (fetal bovine serum) [DMEM (Dulbecco's modified Eagle's medium)/FBS] were compared with those obtained in either SFM or CDM (chemically defined medium). SFM and CDM supported higher cell and mAb concentrations than did DMEM/FBS; however, CE (capillary electrophoresis) analyses revealed important changes in mAb glycosylation patterns. Glycosylation patterns showed a broad microheterogeneity in all the media, ranging from complex to high-mannose and paucimannosidic glycans. mAb produced in DMEM/FBS presented 26 glycan structures, whereas a lower glycan microheterogeneity was found for cultures in CDM or SFM, which presented 24 and 22 structures respectively. In DMEM/FBS and CDM, complex glycans without terminal galactose (G0) represented 28 and 32% of the total glycans respectively and 42 and 46% corresponded to galactosylated structures (G1 plus G2) respectively. In contrast, G0 glycans in SFM accounted for 58%, whereas only 28% corresponded to G1 and G2 structures. Extracellular beta-galactosidase activity increased approx. 3-fold in SFM, which can explain the higher G0 content compared with cultures in the other two media. A desirable decrease in sialylated structures, but an undesirable increase in fucosylated forms, was observed in mAb produced in SFM and CDM media. Approxi. 80% of potential mAb glycosylation sites were occupied, regardless of the culture medium used.

  19. Influence of Indigenous Starter Cultures on the Free Fatty Acids Content During Ripening in Artisan Sausages Produced in the Basilicata Region

    Fabio Favati


    Full Text Available The influence of indigenous starter cultures on the free fatty acids content during ripening of »salsiccia«, a typical dry fermented sausage produced in the Basilicata region, was studied. Three batches of »salsiccia« were produced using different starter mixtures (Lactobacillus sakei G20 and Staphylococcus xylosus S81; L. sakei G20 and S. xylosus S142; L. sakei G20 and S. xylosus S206, while the control batch was produced without a starter. The amounts of free fatty acids present in the samples at the end of the ripening period were not significantly different, suggesting that the lipolytic enzymes naturally occurring in meat could play a predominant role in the free fatty acids release. Oleic and linoleic acids were present in the highest concentrations, while only small quantities of short chain fatty acids were detected, with acetic acid being the most representative one.

  20. Identification and expression analysis of methyl jasmonate responsive ESTs in paclitaxel producing Taxus cuspidata suspension culture cells

    Lenka Sangram K


    Full Text Available Abstract Background Taxol® (paclitaxel promotes microtubule assembly and stabilization and therefore is a potent chemotherapeutic agent against wide range of cancers. Methyl jasmonate (MJ elicited Taxus cell cultures provide a sustainable option to meet the growing market demand for paclitaxel. Despite its increasing pharmaceutical importance, the molecular genetics of paclitaxel biosynthesis is not fully elucidated. This study focuses on identification of MJ responsive transcripts in cultured Taxus cells using PCR-based suppression subtractive hybridization (SSH to identify genes involved in global pathway control. Results Six separate SSH cDNA libraries of paclitaxel-accumulating Taxus cuspidata P991 cell lines were constructed at three different post-elicitation time points (6h, 18h and 5 day to identify genes that are either induced or suppressed in response to MJ. Sequencing of 576 differentially screened clones from the SSH libraries resulted in 331 unigenes. Functional annotation and Gene Ontology (GO analysis of up-regulated EST libraries showed enrichment of several known paclitaxel biosynthetic genes and novel transcripts that may be involved in MJ-signaling, taxane transport, or taxane degradation. Macroarray analysis of these identified genes unravelled global regulatory expression of these transcripts. Semi-quantitative RT-PCR analysis of a set of 12 candidate genes further confirmed the MJ-induced gene expression in a high paclitaxel accumulating Taxus cuspidata P93AF cell line. Conclusions This study elucidates the global temporal expression kinetics of MJ responsive genes in Taxus suspension cell culture. Functional characterization of the novel genes identified in this study will further enhance the understanding of paclitaxel biosynthesis, taxane transport and degradation.

  1. Large scale MALDI-TOF MS based taxa identification to identify novel pigment producers in a marine bacterial culture collection.

    Stafsnes, Marit H; Dybwad, Marius; Brunsvik, Anders; Bruheim, Per


    A challenge in the rational exploitation of microbial culture collections is to avoid superfluous testing of replicas. MALDI-TOF MS has been shown to be an efficient dereplication tool as it can be used to discriminate between bacterial isolates at the species level. A bacterial culture collection of more than 10,000 heterotrophic marine bacterial isolates from sea-water surface layers of the Norwegian Trondheimsfjord and neighbouring coastal areas has been established. A sub-collection of pigmented isolates was earlier screened for novel carotenoids with UVA-Blue light absorbing properties. This was a comprehensive analytical task and it was observed that a significant number of extracts with identical pigment profile were recovered. Hence, this study was undertaken to explore the use of MALDI-TOF MS as a dereplication tool to quickly characterize the bacterial collection. Furthermore, LC-DAD-MS analysis of pigment profiles was performed to check if pigment profile diversity was maintained among isolates kept after the potential MALDI-TOF MS selection step. Four hundred isolates comprising both pigmented and non-pigmented isolates were used for this study. The resulting MALDI-TOF MS dendrogram clearly identified a diversity of different taxa and these were supported by the pigment profile clustering, thus linking the pigment production as species-specific properties. Although one exception was found, it can be concluded that MALDI-TOF MS dereplication is a promising pre-screening tool for more efficient screening of microbial culture collection containing pigments with potential novel properties.

  2. Effect of carbon dioxide and temperature on H2 producing cultures of the extreme thermophile, Caldicellulosiruptor saccharolyticus

    van Niel, Ed; Martens, A.A.; Claassen, P.A.M.


    Carbon dioxide could replace nitrogen gas as stripping gas for the production of H2 by the extreme thermophile, Caldicellulosiruptor saccharolyticus, provided that the pH is kept below 7.0. Above this pH, ≥77 mM sodium bicarbonate will be introduced as an extra salt burden to the culture. High concentrations of sugars and salts contribute each to the inhibition of growth and H2 production, which is most probably due to the osmolality. As a consequence, using CO2 as stripping gas might lower t...

  3. cultural

    Irene Kreutz


    Full Text Available Es un estudio cualitativo que adoptó como referencial teorico-motodológico la antropología y la etnografía. Presenta las experiencias vivenciadas por mujeres de una comunidad en el proceso salud-enfermedad, con el objetivo de comprender los determinantes sócio-culturales e históricos de las prácticas de prevención y tratamiento adoptados por el grupo cultural por medio de la entrevista semi-estructurada. Los temas que emergieron fueron: la relación entre la alimentación y lo proceso salud-enfermedad, las relaciones con el sistema de salud oficial y el proceso salud-enfermedad y lo sobrenatural. Los dados revelaron que los moradores de la comunidad investigada tienen un modo particular de explicar sus procedimientos terapéuticos. Consideramos que es papel de los profesionales de la salud en sus prácticas, la adopción de abordajes o enfoques que consideren al individuo en su dimensión sócio-cultural e histórica, considerando la enorme diversidad cultural en nuestro país.

  4. Bisthiodiketopiperazines and Acorane Sesquiterpenes Produced by the Marine-Derived Fungus Penicillium adametzioides AS-53 on Different Culture Media.

    Liu, Yang; Li, Xiao-Ming; Meng, Ling-Hong; Jiang, Wen-Li; Xu, Gang-Ming; Huang, Cai-Guo; Wang, Bin-Gui


    Chemical investigation of the marine-sponge-derived fungus Penicillium adametzioides AS-53 resulted in the identification of two new bisthiodiketopiperazine derivatives, adametizines A (1) and B (2), from cultivation in a liquid potato-dextrose broth (PDB) culture medium, whereas two new acorane sesquiterpenes, adametacorenols A (3) and B (4), were isolated from a rice solid culture medium. The structures of these compounds were elucidated on the basis of spectroscopic analysis. The absolute configuration of compound 1 was determined by X-ray crystallographic analysis, and that of 3 was determined by modified Mosher's method. Compound 1 exhibited lethality against brine shrimp (Artemia salina) with an LD50 value of 4.8 μM and inhibitory activities against Staphyloccocus aureus, Aeromonas hydrophilia, Vibrio spp. V. harveyi and V. parahaemolyticus, and Gaeumannomyces graminis with minimum inhibitory concentration values of 8, 8, 32, 8, and 16 μg/mL, respectively. Chlorination at C-7 significantly increased the brine shrimp lethality and antimicrobial activity of the bisthiodiketopiperazines.

  5. Identification of N-acyl homoserine lactones produced by Gluconacetobacter diazotrophicus PAL5 cultured in complex and synthetic media.

    Nieto-Peñalver, Carlos G; Bertini, Elisa V; de Figueroa, Lucía I C


    The endophytic diazotrophic Gluconacetobacter diazotrophicus PAL5 was originally isolated from sugarcane (Saccharum officinarum). The biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. The recent genomic sequence of PAL5 unveiled the presence of a quorum sensing (QS) system. QS are regulatory mechanisms that, through the production of signal molecules or autoinducers, permit a microbial population the regulation of the physiology in a coordinated manner. The most studied autoinducers in gram-negative bacteria are the N-acyl homoserine lactones (AHLs). The usage of biosensor strains evidenced the presence of AHL-like molecules in cultures of G. diazotrophicus PAL5 grown in complex and synthetic media. Analysis of AHLs performed by LC-APCI-MS permitted the identification of eight different signal molecules, including C6-, C8-, C10-, C12- and C14-HSL. Mass spectra confirmed that this diazotrophic strain also synthesizes autoinducers with carbonyl substitutions in the acyl chain. No differences in the profile of AHLs could be determined under both culture conditions. However, although the level of short-chain AHLs was not affected, a decrease of 30% in the production of long-chain AHLs could be measured in synthetic medium.

  6. Purification and characterization of pre-S-containing hepatitis B surface antigens produced in recombinant mammalian cell culture.

    Youn, B W; Samanta, H


    Heterogeneous, pre-S-rich HBsAg particles were expressed in recombinant mammalian cell culture and purified to near homogeneity. The purification process comprises: concentration of cell culture medium, protein precipitation by poly(ethylene glycol), gel filtration column chromatography, isopycnic ultracentrifugation by KBr and sucrose density gradient ultracentrifugation. The resulting HBsAg product was greater than 98% pure, and contained much of pre-S1 and pre-S2 components. Scanning densitometry analysis of the silver-stained HBsAg product showed approximately 70-80% S protein, approximately 10-20% pre-S2 protein, and approximately 5-15% pre-S1 protein. It was estimated that the amount of HBV-specific DNA present the final product was less than 7 pg mg-1 HBsAg. Further biochemical analysis has demonstrated that the HBsAg particles are very heterogeneous in charge and density. Charge heterogeneity was quite random among the particles, but density heterogeneity could be related to the different amounts of pre-S2 component in the particles.

  7. Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002.

    Therien, Jesse B; Zadvornyy, Oleg A; Posewitz, Matthew C; Bryant, Donald A; Peters, John W


    The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. Here we demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC 7002. Optimal growth conditions for co-cultivation of C. reinhardtii with wild-type and mutant strains of Synechococcus sp. 7002 were established. In co-culture, acetate produced by a glycogen synthase knockout mutant of Synechococcus sp. PCC 7002 was able to support the growth of a lipid-accumulating mutant strain of C. reinhardtii defective in starch production. Encapsulation of Synechococcus sp. PCC 7002 using an alginate matrix was successfully employed in co-cultures to limit growth and maintain the stability. The ability of immobilized strains of the cyanobacterium Synechococcus sp. PCC 7002 to produce acetate at a level adequate to support the growth of lipid-accumulating strains of C. reinhartdii offers a potentially practical, photosynthetic alternative to providing exogenous acetate into growth media.

  8. Purification and characterization of a collagenolytic enzyme produced by Rathayibacter sp. strains isolated from cultures of Clavibacter michiganensis subsp. michiganensis.

    Labadie, J; Hébraud, M


    We show in this work that collagenolytic Rathayibacter sp. are isolated with phytopathogenic Clavibacter michiganensis subsp. michiganensis strains. The Rathayibacter strains isolated all produced collagenases. One of these collagenases (from the strain 1715) was purified by ammonium sulphate precipitation, DEAE cellulose and Sephadex G 200 chromatography. Characterization of the enzyme showed that it is a true collagenase which is able to degrade both native collagen, gelatin and probably other proteins from plants sharing sequence homologies with collagen.

  9. Evaluation of the Synergistic Effect of Mixed Cultures of White-Rot Fungus Pleurotus ostreatus and Biosurfactant-Producing Bacteria on DDT Biodegradation.

    Purnomo, Adi Setyo; Ashari, Khoirul; Hermansyah, Farizha Triyogi


    DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane) is one of the organic synthetic pesticides that has many negative effects for human health and the environment. The purpose of this study was to investigate the synergistic effect of mixed cutures of white-rot fungus, Pleurotus ostreatus, and biosurfactant-producing bacteria, Pseudomonas aeruginosa and Bacillus subtilis, on DDT biodegradation. Bacteria were added into the P. ostreatus culture (mycelial wet weight on average by 8.53 g) in concentrations of 1, 3, 5, and 10 ml (1 ml ≈ 1.25 × 10(9) bacteria cells/ml culture). DDT was degraded to approximately 19% by P. ostreatus during the 7-day incubation period. The principal result of this study was that the addition of 3 ml of P. aeruginosa into P. ostreatus culture gave the highest DDT degradation rate (approximately 86%) during the 7-day incubation period. This mixed culture combination of the fungus and bacteria also gave the best ratio of optimization of 1.91. DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1-chloro-2,2-bis(4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by P. ostreatus and P. aeruginosa. The results of this study indicate that P. aeruginosa has a synergistic relationship with P. ostreatus and can be used to optimize the degradation of DDT by P. ostreatus.

  10. Mean diameter of nucleolar bodies in cultured human leukemic myeloblasts is mainly related to the S and G2 phase of the cell cycle

    K Smetana


    Full Text Available Mean diameter of nucleolar bodies (nucleoli without the perinucleolar chromatin per cell was studied in human leukemic myeloblasts represented by K 562 and Kasumi 1 cell lines which originated from chronic and acute myeloid leukaemia. The measurement of mean diameter of nucleolar bodies in specimens stained for RNA was very simple. Such approach eliminated the variability of the perinucleolar chromatin discontinuous shell which might influence the measured nucleolar size as suggested by earlier studies. Ageing of K 562 myeloblasts produced a significant decrease of cells in S+G2 phase of the cell cycle accompanied by a significant reduction of mean diameter of nucleolar bodies (MDNoBs per cell. In contrast, treatment of Kasumi 1 myeloblasts with histone deacetylase inhibitor - Trichostatin A - produced a large incidence of resistant cells in S+G2 phase which were characterised by a large increase of MDNoBs. Thus, MDNoBs in leukemic myeloblasts might be a helpful tool to estimate the incidence of cells in the S+G2 phase at the single cell level in smear preparations when the number of cells is very small.


    肖成祖; 黄子才; 张正光; 叶建新; 高丽华; 郭智霞; 程度胜; 周鹤山; 孔惟惟


    CD-1,a genetically-engineered CHO cell line,was cultivated with a Biosilon microcarrier culture system.We successfully cultivated CD-1 cells to a very high density(over 1 ×107 cells/ml).Prourokinase was stably secreted at about 180IU/106 cells/24h.Experiments showed that CD-1 cells growing on Biosilon microcarriers were able to spontaneously release from the microcarriers,then reattach and proliferate on fresh microcarries.This makes it very easy to scale up production.The microcarries could be reused several times without affecting adhesion ,proliferation and prourokinase secretion.With CM-PECC membrane radial flow chromatography and MPG chromatography,the prouroknase in conditioned medium could be purfied to a specific activity of 1×105IU/mg of protein.The purification factor was about 600 fold,and approxiamately 90% of the biological activity was recovered.

  12. Stochastic modelling of Listeria monocytogenes single cell growth in cottage cheese with mesophilic lactic acid bacteria from aroma producing cultures

    Østergaard, Nina Bjerre; Christiansen, Lasse Engbo; Dalgaard, Paw


    for pH, lactic acid concentration and initial concentration of lactic acid bacteria. These data and bootstrap sampling were used to represent product variability in the stochastic model. Lag time data were estimated from observed growth data (lactic acid bacteria) and from literature on L. monocytogenes...... single cells. These lag time data were expressed as relative lag times and included in growth models. A stochastic model was developed from an existing deterministic growth model including the effect of five environmental factors and inter-bacterial interaction [Østergaard, N.B, Eklöw, A and Dalgaard, P....... 2014. Modelling the effect of lactic acid bacteria from starter- and aroma culture on growth of Listeria monocytogenes in cottage cheese. International Journal of Food Microbiology. 188, 15-25]. Growth of L. monocytogenes single cells, using lag time distributions corresponding to three different...

  13. [New anthracycline antibiotics taurimycins A and B produced by a Str. tauricus No. 7036-1 culture].

    Frolova, V I; Kuzovkov, A D; Rozynov, B V; Taĭg, M M; Ivanitskaia, L P


    Taurimycins A and B are new anthracycline antibiotics belonging to the group of glycosides. They were isolated from the mycelium of culture 7036-1 classified with Str. tauricus. The empiral formula of taurimycin A is C14H51O16N,M+813. The empirical formula of taurimycin B is C41H49O16N,M+811. The absorption maxima in the UV spectra of the antibiotics (in alcohol solutions) were observed at 235, 257, 292, 466, 482, 494, 515 and 527 nm. The IR spectra showed absorption bands at 3450, 2945, 2870, 1740 and 1610 cm-1. Hydrolysis of the antibiotics with hydrochloric acid resulted in formation of an aglycone identified as epsilon-pyrromycinone.

  14. Production of sensory compounds by means of the yeast Dekkera bruxellensis in different nitrogen sources with the prospect of producing cachaça.

    Castro Parente, Denise; Vidal, Esteban Espinosa; Leite, Fernanda Cristina Bezerra; de Barros Pita, Will; de Morais, Marcos Antonio


    The distilled spirit made from sugar cane juice, also known as cachaça, is a traditional Brazilian beverage that in recent years has increased its market share among international distilled beverages. Several volatile compounds produced by yeast cells during the fermentation process are responsible for the unique taste and aroma of this drink. The yeast Dekkera bruxellensis has acquired increasing importance in the fermented beverage production, as the different metabolites produced by this yeast may be either beneficial or harmful to the end-product. Since D. bruxellensis is often found in the fermentation processes carried out in ethanol fuel distillation in Brazil, we employed this yeast to analyse the physiological profile and production of aromatic compounds and to examine whether it is feasible to regard it as a cachaça-producing microorganism. The assays were performed on a small scale and simulated the conditions for the production of handmade cachaça. The results showed that the presence of aromatic and branched-chain amino acids in the medium has a strong influence on the metabolism and production of flavours by D. bruxellensis. The assimilation of these alternative nitrogen sources led to different fermentation yields and the production of flavouring compounds. The influence of the nitrogen source on the metabolism of fusel alcohols and esters in D. bruxellensis highlights the need for further studies of the nitrogen requirements to obtain the desired level of sensory compounds in the fermentation. Our results suggest that D. bruxellensis has the potential to play a role in the production of cachaça. Copyright © 2014 John Wiley & Sons, Ltd.

  15. Development and application of a functional CE-SSCP fingerprinting method based on [Fe-Fe]-hydrogenase genes for monitoring hydrogen-producing Clostridium in mixed cultures

    Quemeneur, Marianne; Hamelin, Jerome; Latrille, Eric; Steyer, Jean-Philippe; Trably, Eric [INRA, UR050, Laboratoire de Biotechnologie de l' Environnement, avenue des Etangs, Narbonne F-11100 (France)


    A Capillary Electrophoresis Single Strand Conformation Polymorphism (CE-SSCP) method based on functional [Fe-Fe]-hydrogenase genes was developed for monitoring the hydrogen (H{sub 2})-producing clostridial population in mixed-culture bioprocesses. New non-degenerated primers were designed and then validated on their specific PCR detection of a broad range of clostridial hydA genes. The hydA-based CE-SSCP method gave a specific and discriminating profile for each of the Clostridium strains tested. This method was validated using H{sub 2}-producing mixed cultures incubated at temperatures ranging from 25 C to 45 C. The hydA CE-SSCP profiles clearly differed between temperatures tested. Hence, they varied according to variations of the H{sub 2} production performances. The HydA sequences amplified with the new primer set indicated that diverse Clostridium strains impacted the H{sub 2} production yields. The highest performances were related to the dominance of Clostridium sporogenes-like hydA sequences. This CE-SSCP tool offers highly reliable and throughput analysis of the functional diversity and structure of the hydA genes for better understanding of the H{sub 2}-producing clostridial population dynamics in H{sub 2} dark fermentation bioreactors. (author)

  16. 文学作品翻译中人际意义的跨文化建构%Cross-cultural Construction of Interpersonal Meaning in Literature Translation



    优秀的文学作品通过翻译在全世界广泛流传,以飨各国读者,文学作品的翻译也就承载着实现作者和目的语读者之间的跨文化交际的重任。从功能语言学的一个重要的概念——人际意义的角度考察文学翻译作品,可以发现人际意义的成功传译对译文的质量起着决定性的作用。Halliday的功能语言学将语气和情态作为构成人际意义的主要成分。随着系统功能语言学的发展,人际意义的理论框架得到不断的完善,许多实现人际意义的形式为人们所认识。以系统功能语言学的人际理论为基础,在语气、情态、评价和称谓语四个层面对文学作品翻译中人际意义的跨文化建构问题进行描述和解释,对文学作品的翻译将有所启示。%First-class literature is spread and appreciated in the world through translation.Therefore,literary trans-lation plays an important role in realizing the cross-cultural intercourse between the author and the target language re-ader.As an important concept of Functional Linguistics,the successful translation of the interpersonal meaning de-cides the quality of the translation work.In Halliday's Functional Linguistics,mood and modality are regarded as the principal means in realizing interpersonal meaning.With the development of Systemic Functional Linguistics,the theoretical framework of the interpersonal meaning is improved and much more realizing forms are studied.Based on the interpersonal function theory within the framework of Systemic Functional Linguistics,this essay attempts to discuss the cross-cultural interpersonal meaning in literary translation in terms of mood,modality,evaluation and appellation.It intends to shed lights on literary translation.

  17. IFN-gamma is produced by polymorphonuclear neutrophils in human uterine endometrium and by cultured peripheral blood polymorphonuclear neutrophils.

    Yeaman, G R; Collins, J E; Currie, J K; Guyre, P M; Wira, C R; Fanger, M W


    Cytokines present in the human uterus play an important role both in modulating immune responses to infectious challenge and in the establishment and maintenance of pregnancy. In particular, successful implantation and pregnancy is thought to require the establishment of a Th2 environment, while Th1 cytokines are associated with pregnancy loss and infertility. On the other hand, a Th1 response appears to be required for the resolution of acute infection. Using novel confocal microscopic analysis of fresh sections of human tissue, we have investigated the production of IFN-gamma, a Th1 cytokine, in human endometria. Extracellular IFN-gamma, mostly associated with matrix components, was located immediately beneath the luminal epithelium and along the glandular epithelium proximal to the lumen. As evidenced by intracellular staining, IFN-gamma is produced by both stromal cells and intraepithelial lymphocytes through all stages of the menstrual cycle. Surprisingly, the stromal cell containing intracellular IFN-gamma was identified as a polymorphonuclear neutrophil on the basis of its reactivity with a panel of mAbs and its nuclear morphology. We further found that polymorphonuclear neutrophils isolated from normal donors produce IFN-gamma in response to stimulation with LPS, IL-12, and TNF-alpha. Taken together, these findings suggest that polymorphonuclear neutrophils are capable of producing IFN-gamma both in vitro and in vivo, indicating that their role in shaping immune responses may be more extensive than previously thought. Furthermore, these studies strongly suggest that polymorphonuclear neutrophils play an important role in determining immune responsiveness within the female reproductive tract.

  18. MALDI-TOF characterization of hGH1 produced by hairy root cultures of Brassica oleracea var. italica grown in an airlift with mesh bioreactor.

    López, Edgar García; Ramírez, Emma Gloria Ramos; Gúzman, Octavio Gómez; Calva, Graciano Calva; Ariza-Castolo, Armando; Pérez-Vargas, Josefina; Rodríguez, Herminia Guadalupe Martínez


    Expression systems based on plant cells, tissue, and organ cultures have been investigated as an alternative for production of human therapeutic proteins in bioreactors. In this work, hairy root cultures of Brassica oleracea var. italica (broccoli) were established in an airlift with mesh bioreactor to produce isoform 1 of the human growth hormone (hGH1) as a model therapeutic protein. The hGH1 cDNA was cloned into the pCAMBIA1105.1 binary vector to induce hairy roots in hypocotyls of broccoli plantlets via Agrobacterium rhizogenes. Most of the infected plantlets (90%) developed hairy roots when inoculated before the appearance of true leaves, and keeping the emerging roots attached to hypocotyl explants during transfer to solid Schenk and Hildebrandt medium. The incorporation of the cDNA into the hairy root genome was confirmed by PCR amplification from genomic DNA. The expression and structure of the transgenic hGH1 was assessed by ELISA, western blot, and MALDITOF-MS analysis of the purified protein extracted from the biomass of hairy roots cultivated in bioreactor for 24 days. Production of hGH1 was 5.1 ± 0.42 µg/g dry weight (DW) for flask cultures, and 7.8 ± 0.3 µg/g DW for bioreactor, with productivity of 0.68 ± 0.05 and 1.5 ± 0.06 µg/g DW*days, respectively, indicating that the production of hGH1 was not affected by the growth rate, but might be affected by the culture system. These results demonstrate that hairy root cultures of broccoli have potential as an alternative expression system for production of hGH1, and might also be useful for production of other therapeutic proteins.

  19. Producing access for the elderly to territories of culture: an experience of occupational therapy in an art museum

    Ana Tereza Costa Galvanese


    Full Text Available From 1996 to 2009, the Laboratory for Studies and Research in Art, Body and Occupational Therapy established a cooperation with the Museum of Contemporary Art of USP (MAC USP, working in partnership with the Leisure and Art to the Elderly Program of the Education and Technical-Scientific Division of MAC USP. The program offers an introduction in contemporary artistic practice to the elderly. This paper presents the interdisciplinary experience developed in this partnership in 2006. The method adopted in the program is referenced in the Triangular Approach to Teaching Art. Therefore, the appreciation of works of art and the contextualization of selected artists formed the basis on which participants developed their own poetics. The preparatory work was developed in group dynamics, including activities of body awareness and conversation circles coordinated by occupational therapists and students. They also accompanied the participants in their demands related to the challenges of constructing access to socio-cultural territories. The relevance of this living process was evident in the topics proposed by participants in conversations, or arisen during the body work. The aesthetic quality of the participants’ production resulted in personal and collective satisfaction and provoked admiration of the public who visited the workshop and exhibition, organized from this production.


    Shchekoldina T. V.


    Full Text Available Healthy nutrition is the foundation of the nation and the most important factor affecting the length of human life. Therefore, the search and the use of new sources of food are important. One of such source is the ancient culture of quinoa (Chenopodium quinoa, known as a "qinoa", "quinva" or "rice quinoa. The uniqueness of the quinoa is its chemical composition, the absence of anti-nutritional substances and unpretentious in growing and harvesting. Currently quinoa is grown in more than 70 countries. In Russia, quinoa is not cultivated, although the presence of different agro-ecological zones of the plains to the highlands allows it to grow. In April 2014 quinoa was first planted in the Krasnodar region. In September, quinoa whisk acquired red and yellow. The seeds have a size of between 1 and 2.5 mm. Preliminary calculations yield was approximately 39hundredweight / hectare, which is the basis for the introduction of quinoa in a mass planting. Thus, quinoa can be grown in Russia in the Krasnodar region, thereby ensuring its own raw material base for development of new specialized foods (gluten-free and prophylactic (high nutritional value destination

  1. Optimization of Culture Conditions for Some Identified Fungal Species and Stability Profile of α-Galactosidase Produced.

    Chauhan, A S; Srivastava, N; Kehri, H K; Sharma, B


    Microbial α-galactosidase preparations have implications in medicine and in the modification of various agricultural products as well. In this paper, four isolated fungal strains such as AL-3, WF-3, WP-4 and CL-4 from rhizospheric soil identified as Penicillium glabrum (AL-3), Trichoderma evansii (WF-3), Lasiodiplodia theobromae (WP-4) and Penicillium flavus (CL-4) based on their morphology and microscopic examinations, are screened for their potential towards α-galactosidases production. The culture conditions have been optimized and supplemented with specific carbon substrates (1%, w/v) by using galactose-containing polysaccharides like guar gum (GG), soya casein (SC) and wheat straw (WS). All strains significantly released galactose from GG, showing maximum production of enzyme at 7th day of incubation in rotary shaker (120 rpm) that is 190.3, 174.5, 93.9 and 28.8 U/mL, respectively, followed by SC and WS. The enzyme activity was stable up to 7days at -20°C, then after it declines. This investigation reveals that AL-3 show optimum enzyme activity in guar gum media, whereas WF-3 exhibited greater enzyme stability. Results indicated that the secretion of proteins, enzyme and the stability of enzyme activity varied not only from one strain to another but also differed in their preferences of utilization of different substrates.

  2. Optimization of Culture Conditions for Some Identified Fungal Species and Stability Profile of α-Galactosidase Produced

    A. S. Chauhan


    Full Text Available Microbial α-galactosidase preparations have implications in medicine and in the modification of various agricultural products as well. In this paper, four isolated fungal strains such as AL-3, WF-3, WP-4 and CL-4 from rhizospheric soil identified as Penicillium glabrum (AL-3, Trichoderma evansii (WF-3, Lasiodiplodia theobromae (WP-4 and Penicillium flavus (CL-4 based on their morphology and microscopic examinations, are screened for their potential towards α-galactosidases production. The culture conditions have been optimized and supplemented with specific carbon substrates (1%, w/v by using galactose-containing polysaccharides like guar gum (GG, soya casein (SC and wheat straw (WS. All strains significantly released galactose from GG, showing maximum production of enzyme at 7th day of incubation in rotary shaker (120 rpm that is 190.3, 174.5, 93.9 and 28.8 U/mL, respectively, followed by SC and WS. The enzyme activity was stable up to 7days at −20°C, then after it declines. This investigation reveals that AL-3 show optimum enzyme activity in guar gum media, whereas WF-3 exhibited greater enzyme stability. Results indicated that the secretion of proteins, enzyme and the stability of enzyme activity varied not only from one strain to another but also differed in their preferences of utilization of different substrates.

  3. Urine culture

    Culture and sensitivity - urine ... when urinating. You also may have a urine culture after you have been treated for an infection. ... when bacteria or yeast are found in the culture. This likely means that you have a urinary ...

  4. I spy with my little eye something beginning with O: looking at what the myth of 'doing the observations' means in mental health nursing culture.

    Holyoake, D-D


    Those who are familiar with psychiatric inpatient settings will be aware of the expressions 'doing the obs', 'being on checks' and 'special observations'. That is because the task of observing patients is seen as being pivotal to the mental health nursing role. This paper describes an ethnographic research project that offers a rethinking of psychiatric observation. The author uses data from an ethnographic research project to provide an examination of the structure, process and outcome of this seemingly straightforward nursing task and explores how 'doing the obs' has additional symbolic and cultural meaning similar to what Barthes terms myth. The symbolic connotations are numerous and wide-ranging and expose a practice that could be said to punctuate daily activities and an ordering of relationships between nurses and services users. The classic sociological issues of status, power and containment are all relevant, yet rethought. The use of ethnographic research allowed the author to focus on the more symbolic cultural dynamics and develop five initial ethnographic themes concerning the constituting experience of watching and being watched.

  5. Biogenic amine content in dry fermented sausages as influenced by a producer, spice mix, starter culture, sausage diameter and time of ripening.

    Komprda, T; Sládková, P; Dohnal, V


    Sixteen types of dry fermented sausages were commercially produced as combinations of two producers (designated K and R), two starter cultures (Pediococcus pentosaceus, C; Lactobacillus curvatus+Staphylococcus carnosus, F), two spicing mixtures (H; P) and two casing diameters (4.5cm, T; 7cm, W), and were sampled at days zero, 14, 28 (end of ripening), 49, 70, 91 and 112 (samples were stored at 15°C and relative humidity of 70% between days 28 and 112). Tyramine and putrescine content (Y, mgkg(-1)) increased (Psausages as compared to the W, H and F counterparts, respectively; content of both amines was lower (Psausages than in the R-sausages. Tyramine content in the sausages at the time interval 28days of ripening+21days of storage was in the range from 170 (KHCU sausage combination) to 382 (RHFS) mgkg(-1).

  6. Plasma cytokine concentration and the cytokine producing ability of whole blood cell cultures from healthy females with pharmacologically induced hyperprolactinemia.

    Rovenský, J; Lackovic, V; Veselková, Z; Horváthová, M; Koska, J; Blazícková, S; Vigas, M


    We investigated the in vitro effect of domperidone-induced hyperprolactinemia on plasma cytokine concentration and blood leukocyte cytokine production in healthy female volunteers. No changes were found in the plasma concentration of interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, interleukin (IL)-4, IL-10, IL-6 and IL-13 during hyperprolactinemia when compared with control values. Using unseparated blood leukocytes, we found that the spontaneous production of IL-6 (4-8 h) and transforming growth factor (TGF)-beta 1 (2-4 h) was significantly decreased and that the in vitro stimulated production of IFN-gamma (2-8 h) and TNF (4 h) was significantly increased compared with control. Our data concerning the increased IFN-gamma and TNF producing capacity of unseparated leukocytes during pharmacologically induced hyperprolactinemia strongly support the possibility that the lymphocyte production of these cytokines can be rapidly amplified by prolactin via a priming mechanism.

  7. Preliminary viability studies of fibroblastic cells cultured on microcrystalline and nanocrystalline diamonds produced by chemical vapour deposition method

    Ana Amélia Rodrigues


    Full Text Available Implant materials used in orthopedics surgery have demonstrated some disadvantages, such as metallic corrosion processes, generation of wear particles, inflammation reactions and bone reabsorption in the implant region. The diamond produced through hot-filament chemical vapour deposition method is a new potential biomedical material due to its chemical inertness, extreme hardness and low coefficient of friction. In the present study we analysis two samples: the microcrystalline diamond and the nanocrystalline diamond. The aim of this study was to evaluate the surface properties of the diamond samples by scanning electron microscopy, Raman spectroscopy and atomic force microscopy. Cell viability and morphology were assessed using thiazolyl blue tetrazolium bromide, cytochemical assay and scanning electron microscopy, respectively. The results revealed that the two samples did not interfere in the cell viability, however the proliferation of fibroblasts cells observed was comparatively higher with the nanocrystalline diamond.

  8. Preliminary viability studies of fibroblastic cells cultured on microcrystalline and nanocrystalline diamonds produced by chemical vapour deposition method

    Ana Amélia Rodrigues


    Full Text Available Implant materials used in orthopedics surgery have demonstrated some disadvantages, such as metallic corrosion processes, generation of wear particles, inflammation reactions and bone reabsorption in the implant region. The diamond produced through hot-filament chemical vapour deposition method is a new potential biomedical material due to its chemical inertness, extreme hardness and low coefficient of friction. In the present study we analysis two samples: the microcrystalline diamond and the nanocrystalline diamond. The aim of this study was to evaluate the surface properties of the diamond samples by scanning electron microscopy, Raman spectroscopy and atomic force microscopy. Cell viability and morphology were assessed using thiazolyl blue tetrazolium bromide, cytochemical assay and scanning electron microscopy, respectively. The results revealed that the two samples did not interfere in the cell viability, however the proliferation of fibroblasts cells observed was comparatively higher with the nanocrystalline diamond.

  9. A versatile, non genetically modified organism (GMO)-based strategy for controlling low-producer mutants in Bordetella pertussis cultures using antigenic modulation.

    Goffin, Philippe; Slock, Thomas; Smessaert, Vincent; De Rop, Philippe; Dehottay, Philippe


    The uncontrolled presence of non-producer mutants negatively affects bioprocesses. In Bordetella pertussis cultures, avirulent mutants emerge spontaneously and accumulate. We characterized the dynamics of accumulation using high-throughput growth assays and competition experiments between virulent and avirulent (bvg(-) ) isolates. A fitness advantage of bvg(-) cells was identified as the main driver for bvg(-) accumulation under conditions of high virulence factor production. Conversely, under conditions that reduce their expression (antigenic modulation), bvg(-) takeover could be avoided. A control strategy was derived, which consists in applying modulating conditions whenever virulence factor production is not required. It has a wide range of applications, from routine laboratory operations to vaccine manufacturing, where pertussis toxin yields were increased 1.4-fold by performing early pre-culture steps in modulating conditions. Because it only requires subtle modifications of the culture medium and does not involve genetic modifications, this strategy is applicable to any B. pertussis isolate, and should facilitate regulatory acceptance of process changes for vaccine production. Strategies based on the same concept, could be derived for other industrially relevant micro-organisms. This study illustrates how a sound scientific understanding of physiological principles can be turned into a practical application for the bioprocess industry, in alignment with Quality by Design principles. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Interaction between season and culture with insulin-like growth factor-1 on survival of in vitro produced embryos following transfer to lactating dairy cows.

    Block, J; Hansen, P J


    Culture of bovine embryos in the presence of insulin-like growth factor-1 (IGF-1) can increase pregnancy rates following transfer to heat-stressed, lactating dairy cows. The objective of the present experiment was to determine whether the effect of IGF-1 on post-transfer embryo survival was a general effect or one specific to heat stress. Lactating recipients (n=311) were synchronized for timed-embryo transfer at four locations. Embryos were produced in vitro and cultured with or without 100 ng/mL IGF-1. At Day 7 after anticipated ovulation (Day 0), a single embryo was randomly transferred to each recipient. Pregnancy was diagnosed at Day 21 by elevated plasma progesterone concentrations, at Days 27-32 by ultrasonography, and at Days 41-49 by transrectal palpation. Transfers were categorized into two seasons, hot or cool (based on the month of transfer). There was a tendency (Pcalving rate (Pcalves born was 77.5%. In conclusion, treatment of embryos with IGF-1 improved pregnancy and calving rates following the transfer of in vitro produced embryos into lactating recipients, but only under heat-stress conditions.

  11. Efficacy of in vitro embryo transfer in lactating dairy cows using fresh or vitrified embryos produced in a novel embryo culture medium.

    Block, J; Bonilla, L; Hansen, P J


    Objectives were to determine whether pregnancy success could be improved in lactating cows with timed embryo transfer when embryos were produced in vitro using a medium designed to enhance embryo development and survival after cryopreservation. In experiment 1, embryos (n=569 to 922) were cultured in either modified synthetic oviduct fluid or a serum-free medium, Block-Bonilla-Hansen-7 (BBH7). Development to the blastocyst stage was recorded at d 7, and selected blastocysts (n=79 to 114) were vitrified using open pulled straws. Culture of embryos in BBH7 increased development to the blastocyst stage (41.9±2.0 vs. 14.7±2.0%) and advanced blastocyst stages (expanded, hatching, hatched; 31.1±1.3 vs. 6.4±1.3%) at d 7 and resulted in higher hatching rates at 24h postwarming compared with embryos cultured in modified synthetic oviduct fluid (59.0±0.5 vs. 26.7±0.5%). In experiment 2, embryos were produced using X-sorted semen and cultured in BBH7. At d 7 after insemination, embryos were transferred fresh or following vitrification. Lactating Holstein cows were either subjected to timed artificial insemination (TAI) on the day of presumptive ovulation or used as embryo recipients 7 d later. Embryo recipients received an embryo if a corpus luteum was present. The percentage of cows pregnant at d 32, 46, and 76 of gestation was higher among cows that received fresh embryos compared with TAI cows or cows that received vitrified embryos. At d 76, for example, the proportion and percentage pregnant was 47/150 (31.3%) for cows subjected to TAI, 48/95 (50.5%) for cows receiving fresh embryos, and 39/141 (27.7%) for cows receiving a vitrified embryo. No difference was observed in the percentage of cows pregnant among TAI cows and those that received vitrified embryos. There was a service or transfer number × treatment interaction because differences in pregnancy rate between embryo transfer recipients and cows bred by TAI were greater for cows with more than 3 services or


    Muhammad Asgher,


    Full Text Available Lignin peroxidase was produced from Schyzophyllum commune IBL-06 through solid state fermentation of an abundantly available agro-industrial waste, banana stalk, under pre-optimized conditions. LiP was fractionated by 65% saturation with NH4SO4 and dialysis to 1.5-fold purification. The enzyme was further purified by Sephadex G-100 gel filtration chromatography to 2.34 fold with specific activity of 468 U/mg. A single band of 80 kDa was obtained on native gel while on sodium dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE, and two bands having molecular weight of 33 & 47 kDa were obtained, suggesting that LiP was a two polypeptide oligomeric protein. The present LiP from S. commune IBL-06 was optimally active at pH 5 and 35°C. The stability assay showed that LiP retained activity in an acidic pH range of 4 to 6 and a temperature of 25 to 45°C after 24 h of incubation. Lignin peroxidase oxidized the vertry alcohol and showed kinetic constants KM and Vmax values of 0.46 mM and 388 mM/min, respectively. All organic and inorganic compounds inhibited S. commune LiP, but EDTA, β-Marcaptoethanol, and Pb(NO32 were the most inhibitory.

  13. Experimental investigation on spontaneously active hippocampal cultures recorded by means of high-density MEAs: analysis of the spatial resolution effects

    Alessandro Maccione


    Full Text Available Based on experiments performed with high-resolution Active Pixel Sensor microelectrode arrays (APS-MEAs coupled with spontaneously active hippocampal cultures, this work investigates the spatial resolution effects of the neuroelectronic interface on the analysis of the recorded electrophysiological signals. The adopted methodology consists, first, in recording the spontaneous activity at the highest spatial resolution (inter-electrode separation of 21 µm from the whole array of 4096 microelectrodes. Then, the full resolution dataset is spatially down sampled in order to evaluate the effects on raster plot representation, array-wide spike rate (AWSR, mean firing rate (MFR and mean bursting rate (MBR. Furthermore, the effects of the array-to-network relative position are evaluated by shifting a subset of equally spaced electrodes on the entire recorded area. Results highlight that MFR and MBR are particularly influenced by the spatial resolution provided by the neuroelectronic interface. On high-resolution large MEAs, such analysis better represent the time-based parameterization of the network dynamics. Finally, this work suggest interesting capabilities of high-resolution MEAs for spatial-based analysis in dense and low-dense neuronal preparation for investigating signalling at both local and global neuronal circuitries.

  14. Methodological aspects of a pattern-scaling approach to produce global fields of monthly means of daily maximum and minimum temperature

    Kremser, S.; Bodeker, G. E.; Lewis, J.


    A Climate Pattern-Scaling Model (CPSM) that simulates global patterns of climate change, for a prescribed emissions scenario, is described. A CPSM works by quantitatively establishing the statistical relationship between a climate variable at a specific location (e.g. daily maximum surface temperature, Tmax) and one or more predictor time series (e.g. global mean surface temperature, Tglobal) - referred to as the "training" of the CPSM. This training uses a regression model to derive fit coefficients that describe the statistical relationship between the predictor time series and the target climate variable time series. Once that relationship has been determined, and given the predictor time series for any greenhouse gas (GHG) emissions scenario, the change in the climate variable of interest can be reconstructed - referred to as the "application" of the CPSM. The advantage of using a CPSM rather than a typical atmosphere-ocean global climate model (AOGCM) is that the predictor time series required by the CPSM can usually be generated quickly using a simple climate model (SCM) for any prescribed GHG emissions scenario and then applied to generate global fields of the climate variable of interest. The training can be performed either on historical measurements or on output from an AOGCM. Using model output from 21st century simulations has the advantage that the climate change signal is more pronounced than in historical data and therefore a more robust statistical relationship is obtained. The disadvantage of using AOGCM output is that the CPSM training might be compromised by any AOGCM inadequacies. For the purposes of exploring the various methodological aspects of the CPSM approach, AOGCM output was used in this study to train the CPSM. These investigations of the CPSM methodology focus on monthly mean fields of daily temperature extremes (Tmax and Tmin). The methodological aspects of the CPSM explored in this study include (1) investigation of the advantage

  15. Isolation of TDA-producing Phaeobacter strains from sea bass larval rearing units and their probiotic effect against pathogenic Vibrio spp. in Artemia cultures.

    Grotkjær, Torben; Bentzon-Tilia, Mikkel; D'Alvise, Paul; Dourala, Nancy; Nielsen, Kristian Fog; Gram, Lone


    Fish-pathogenic Vibrio can cause large-scale crashes in marine larval rearing units and, since the use of antibiotics can result in bacterial antibiotic resistance, new strategies for disease prevention are needed. Roseobacter-clade bacteria from turbot larval rearing facilities can antagonize Vibrio anguillarum and reduce mortality in V. anguillarum-infected cod and turbot larvae. In this study, it was demonstrated that antagonistic Roseobacter-clade bacteria could be isolated from sea bass larval rearing units. In addition, it was shown that they not only antagonized V. anguillarum but also V. harveyi, which is the major bacterial pathogen in crustaceans and Mediterranean sea bass larvae cultures. Concomitantly, they significantly improved survival of V. harveyi-infected brine shrimp. 16S rRNA gene sequence homology identified the antagonists as Phaeobacter sp., and in silico DNA-DNA hybridization indicated that they could belong to a new species. The genomes contained genes involved in synthesis of the antibacterial compound tropodithietic acid (TDA), and its production was confirmed by UHPLC-TOFMS. The new Phaeobacter colonized live feed (Artemia) cultures and reduced Vibrio counts significantly, since they reached only 10(4)CFUmL(-1), as opposed to 10(8)CFUmL(-1) in non-Phaeobacter treated controls. Survival of V. anguillarum-challenged Artemia nauplii was enhanced by the presence of wild type Phaeobacter compared to challenged control cultures (89±1.0% vs 8±3.2%). In conclusion, TDA-producing Phaeobacter isolated from Mediterranean marine larviculture are promising probiotic bacteria against pathogenic Vibrio in crustacean live-feed cultures for marine fish larvae.

  16. 曼谷唐人街的空间生产与族群文化交流%The Space Produce and Ethnic Culture Communication in Chinatown of Bangkok



    Chinese people have been immigrating into Thailand since ancient time in the history.Chinese people have now taken up more than one tenth of the total population in Thailand.Chinatown has become the most important social space for ethnic culture communication between Chinese and Thai.Selling Chinese goods and intermarriage are the important forms of clan culture communication.The construction of public temples is the way for Chinese community to produce the space.Due to the basis of cultural difference,it′s hard for assimilation of Chinese to Thai society on one hand,and on the other hand,the space production never seems to end.But the new life style and cultural achievement will be flourishing as the result of the space production.%历史上华人很早就移居泰国。泰国华人现已占泰国人口的十分之一以上。在泰国,唐人街是华人与泰人进行文化与经济交流的社会空间,销售中国物品、族际通婚等是其族群文化交流的重要形式,而公庙的建设则系华人社区公共空间生产的重要方式。由于文化根基的不同,唐人街华人与泰人社区不会因为交融的深入而消弭,空间的生产也不会停止,但新的生活方式与文化成果必然会随空间生产而璀璨绽放。

  17. Hyperspectral imaging for detection of non-O157 Shiga-toxin producing Escherichia coli (STEC) serogroups on spread plates of mixed cultures

    Yoon, Seung Chul; Windham, William R.; Ladely, Scott; Heitschmidt, Gerald W.; Lawrence, Kurt C.; Park, Bosoon; Narang, Neelam; Cray, William C.


    We investigated the feasibility of visible and near-infrared (VNIR) hyperspectral imaging for rapid presumptive-positive screening of six representative non-O157 Shiga-toxin producing Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, and O145) on spread plates of mixed cultures. Although the traditional culture method is still the "gold standard" for presumptive-positive pathogen screening, it is time-consuming, labor-intensive, not effective in testing large amount of food samples, and cannot completely prevent unwanted background microflora from growing together with target microorganisms on agar media. A previous study was performed using the data obtained from pure cultures individually inoculated on spot and/or spread plates in order to develop multivariate classification models differentiating each colony of the six non-O157 STEC serogroups and to optimize the models in terms of parameters. This study dealt with the validation of the trained and optimized models with a test set of new independent samples obtained from colonies on spread plates of mixed cultures. A new validation protocol appropriate to a hyperspectral imaging study for mixed cultures was developed. One imaging experiment with colonies obtained from two serial dilutions was performed. A total of six agar plates were prepared, where O45, O111 and O121 serogroups were inoculated into all six plates and each of O45, O103 and O145 serogroups was added into the mixture of the three common bacterial cultures. The number of colonies grown after 24-h incubation was 331 and the number of pixels associated with the grown colonies was 16,379. The best model found from this validation study was based on pre-processing with standard normal variate and detrending (SNVD), first derivative, spectral smoothing, and k-nearest neighbor classification (kNN, k=3) of scores in the principal component subspace spanned by 6 principal components. The independent testing results showed 95% overall

  18. Antimicrobial residue detection in chicken yolk samples following administration to egg-producing chickens and effects of residue detection on competitive exclusion culture (PREEMPT) establishment.

    McReynolds, J L; Caldwell, D Y; McElroy, A P; Hargis, B M; Caldwell, D J


    in chicks derived from enrofloxacin-treated hens at either collection period. Although several antibiotics do not appear to produce detectable egg residues or interfere with CE culture establishment, these data suggest that chicks derived from enrofloxacin-treated hens may not be candidates for safe and effective CE culture treatment.

  19. Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cell.

    Stuelsatz, Pascal; Keire, Paul; Yablonka-Reuveni, Zipora


    Multinucleated myofibers, the functional contractile units of adult skeletal muscle, harbor mononuclear Pax7(+) myogenic progenitors on their surface between the myofiber basal lamina and plasmalemma. These progenitors, known as satellite cells, are the primary myogenic stem cells in adult muscle. This chapter describes our laboratory protocols for isolating, culturing, and immunostaining intact myofibers from mouse skeletal muscle as a means for studying satellite cell dynamics. The first protocol discusses myofiber isolation from the flexor digitorum brevis (FDB) muscle. These short myofibers are plated in dishes coated with PureCol collagen (formerly known as Vitrogen) and maintained in a mitogen-poor medium (± supplemental growth factors). Employing such conditions, satellite cells remain at the surface of the parent myofiber while synchronously undergoing a limited number of proliferative cycles and rapidly differentiate. The second protocol discusses the isolation of longer myofibers from the extensor digitorum longus (EDL) muscle. These EDL myofibers are routinely plated individually as adherent myofibers in wells coated with Matrigel and maintained in a mitogen-rich medium, conditions in which satellite cells migrate away from the parent myofiber, proliferate extensively, and generate numerous differentiating progeny. Alternatively, these EDL myofibers can be plated as non-adherent myofibers in uncoated wells and maintained in a mitogen-poor medium (± supplemental growth factors), conditions that retain satellite cell progeny at the myofiber niche similar to the FDB myofiber cultures. However, the adherent myofiber format is our preferred choice for monitoring satellite cells in freshly isolated (Time 0) myofibers. We conclude this chapter by promoting the Nestin-GFP transgenic mouse as an efficient tool for direct analysis of satellite cells in isolated myofibers. While satellite cells have been often detected by their expression of the Pax7 protein or

  20. Alternative anaerobic enrichments to the bacteriological analytical manual culture method for isolation of Shigella sonnei from selected types of fresh produce.

    Jacobson, Andrew P; Thunberg, Richard L; Johnson, Mildred L; Hammack, Thomas S; Andrews, Wallace H


    Alternative methods of reducing oxygen during anaerobic enrichment in the Bacteriological Analytical Manual (BAM) Shigella culture method were evaluated and compared to the current and less practical GasPak method. The alternative anaerobic methods included the use of reducing agents in Shigella broth and reducing culture container headspace volume to minimize atmospheric effects on oxygen concentration in Shigella broth during enrichment. The reducing agents evaluated were sodium thioglycollate, L-cystine, L-cysteine, titanium(III) citrate, and dithiothreitol, each at concentrations of 0.1, 0.05, and 0.01%. The use of Oxyrase for Broth with the enrichment medium (Shigella broth) was evaluated at concentrations of 10, 20 and 30 microL/mL. Recoveries of chill- and freeze-stressed S. sonnei strains 357 and 20143 were determined with each anaerobic method, including the GasPak method, using inoculation levels ranging from 10(0)to 10(3) cells. For each anaerobic method, strain, inoculation level, and stress type, 5 replicate enrichments were evaluated by streaking to MacConkey agar for isolation. The numbers of cultures with each method from which S. sonnei was isolated were used to compare the alternative anaerobic methods to the GasPak method. The alternative anaerobic method with which chill- and freeze-stressed S. sonnei strains 357 and 20143 were isolated most consistently was the use of Oxyrase for Broth in Shigella broth at a concentration of 20 microL/mL. This method was compared to the GasPak anaerobic method in evaluations on the recovery of S. sonnei strains 357 and 20143 from artificially contaminated test portions of parsley, cilantro, green onions, strawberries, carrots, and celery. A third anaerobic method included the use of 0.5 cm mineral oil overlay on cultures containing Oxyrase for Broth at concentrations of 20 microL/mL. Recovery rates of strain 357 were significantly greater (p 0.05) between the GasPak and Oxyrase for Broth anaerobic methods

  1. Perspective-Taking and Meaning-Making through Engagement with Cultural Narratives: Bringing History to Life in a Foreign Language Classroom


    The MLA Report (2007) accords considerable weight to the role of culture in a transformed approach to language education in the U.S. and outlines “one possible model” for developing transculturalunderstanding that involves the interpretation of the “cultural narratives” inherent in all forms of cultural representation (p. 238). How exactly students might be engaged in interpreting cultural narratives in the foreign language classroom, though, remains to be further specified, imagined, practic...

  2. 西方文化中人的尊严的涵义及其演化%The Meanings of Human Dignity and Its Evolution in Western Culture



    The meanings of human dignity was developed constantly from ancient Roma where it was origina-ted to modern society in western culture.Accordingly,the ethical emphasis were different in different era.In western traditional culture,the word dignity was mainly used in religion and philosophy,focused on every person should earn his own dignity by pursuing excellence in reason and morality.One’s dignity is not equal to the oth-er’s.Every person was responsible for his own dignity.In modern society,the word dignity is mainly used in politics,jurisprudence and bioethics.Although it is used as the foundation of human rights,its meanings is vague,focus on everyone has inherent dignity because everyone as human being is superior to other animals. Everyone has equal dignity.Others are responsible for one’s dignity,Today,the idea of human dignity in west-ern traditional culture is challenged overall,how to respond is a subject which is worth to study.%当今被广泛使用的尊严一词是一个历史的概念。从古罗马时期起源,经历中世纪和文艺复兴时期,再到启蒙运动时期,直到现当代,其涵义是逐渐变化的。相应地,其在伦理学上的侧重点也不同。在西方传统文化中,尊严一词主要用于宗教和哲学领域,其涵义相对固定,侧重于每个人要活出自己的尊严,即强调每个人自己要追求理性和道德上的卓越,使自己获得尊严。这种尊严在人和人之间是不平等的。个人对自己的尊严负责。在现当代,尊严一词主要用在政治、法学、生命伦理领域,虽然它被用来作为人权的基础,然而其涵义却是相当模糊和不确定的,侧重于每个人作为人类一员生而具有的高于动物的固有的尊严,这种尊严是人人平等享有的。别人要对自己的尊严负责。今天,西方文化传统中的人的尊严理念受到全面挑战,如何回应值得进一步研究。

  3. Screening of endophytic sources of exopolysaccharides: Preliminary characterization of crude exopolysaccharide produced by submerged culture of Diaporthe sp. JF766998 under different cultivation time

    Ravely Casarotti Orlandelli


    Full Text Available Endophytic fungi have been described as producers of important bioactive compounds; however, they remain under-exploited as exopolysaccharides (EPS sources. Therefore, this work reports on EPS production by submerged cultures of eight endophytes isolated from Piper hispidum Sw., belonging to genera Diaporthe, Marasmius, Phlebia, Phoma, Phyllosticta and Schizophyllum. After fermentation for 96 h, four endophytes secreted EPS: Diaporthe sp. JF767000, Diaporthe sp. JF766998, Diaporthe sp. JF767007 and Phoma herbarum JF766995. The EPS from Diaporthe sp. JF766998 differed statistically from the others, with a higher percentage of carbohydrate (91% and lower amount of protein (8%. Subsequently, this fungus was grown under submerged culture for 72, 96 and 168 h (these EPS were designated EPSD1-72, EPSD1-96 and EPSD1-168 and the differences in production, monosaccharide composition and apparent molecular were compared. The EPS yields in mg/100 mL of culture medium were: 3.0 ± 0.4 (EPSD1-72, 15.4 ± 2.2 (EPSD1-96 and 14.8 ± 1.8 (EPSD1-168. The EPSD1-72 had high protein content (28.5% and only 71% of carbohydrate; while EPSD1-96 and EPSD1-168 were composed mainly of carbohydrate (≈95 and 100%, respectively, with low protein content (≈5% detected at 96 h. Galactose was the main monosaccharide component (30% of EPSD1-168. Differently, EPSD1-96 was rich in glucose (51%, with molecular weight of 46.6 kDa. It is an important feature for future investigations, because glucan-rich EPS are reported as effective antitumor agents.

  4. Simultaneous clarification of Escherichia coli culture and purification of extracellularly produced penicillin G acylase using tangential flow filtration and anion-exchange membrane chromatography (TFF-AEMC).

    Orr, Valerie; Scharer, Jeno; Moo-Young, Murray; Honeyman, C Howie; Fenner, Drew; Crossley, Lisa; Suen, Shing-Yi; Chou, C Perry


    Downstream purification often represents the most cost-intensive step in the manufacturing of recombinant proteins since conventional purification processes are lengthy, technically complicated, and time-consuming. To address this issue, herein we demonstrated the simultaneous clarification and purification of the extracellularly produced recombinant protein by Escherichia coli using an integrated system of tangential flow filtration and anion exchange membrane chromatography (TFF-AEMC). After cultivation in a bench-top bioreactor with 1L working volume using the developed host/vector system for high-level expression and effective secretion of recombinant penicillin G acylase (PAC), the whole culture broth was applied directly to the established system. One-step purification of recombinant PAC was achieved based on the dual nature of membrane chromatography (i.e. microfiltration-sized pores and anion-exchange chemistry) and cross-flow operations. Most contaminant proteins in the extracellular medium were captured by the anion-exchange membrane and cells remained in the retentate, whereas extracellular PAC was purified and collected in the filtrate. The batch time for both cultivation and purification was less than 24h and recombinant PAC with high purity (19 U/mg), yield (72% recovery), and productivity (41 mg of purified PAC per liter of culture) was obtained. Due to the nature of the non-selective protein secretion system and the versatility of ion-exchange membrane chromatography, the developed system can be widely applied for effective production and purification of recombinant proteins.

  5. Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF

    Coppock Donald L


    Full Text Available Abstract Background Multiple physiologic impairments are responsible for chronic wounds. A cell line grown which retains its phenotype from patient wounds would provide means of testing new therapies. Clinical information on patients from whom cells were grown can provide insights into mechanisms of specific disease such as diabetes or biological processes such as aging. The objective of this study was 1 To culture human cells derived from patients with chronic wounds and to test the effects of putative therapies, Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF on these cells. 2 To describe a methodology to create fibroblast cell lines from patients with chronic wounds. Methods Patient biopsies were obtained from 3 distinct locations on venous ulcers. Fibroblasts derived from different wound locations were tested for their migration capacities without stimulators and in response to GM-CSF. Another portion of the patient biopsy was used to develop primary fibroblast cultures after rigorous passage and antimicrobial testing. Results Fibroblasts from the non-healing edge had almost no migration capacity, wound base fibroblasts were intermediate, and fibroblasts derived from the healing edge had a capacity to migrate similar to healthy, normal, primary dermal fibroblasts. Non-healing edge fibroblasts did not respond to GM-CSF. Six fibroblast cell lines are currently available at the National Institute on Aging (NIA Cell Repository. Conclusion We conclude that primary cells from chronic ulcers can be established in culture and that they maintain their in vivo phenotype. These cells can be utilized for evaluating the effects of wound healing stimulators in vitro.

  6. Low-cost mobile hatchery produced silver barb (Barbodes gonionotus) fingerling:it's growth and economic performance in intensive culture system

    SM Mustafizur Rahman; Biplob Basak; Siddhwartha Kumar Basak; Md Rushna Alam; Md Mohsin Ali; Mohammad Mahfujul Haque


    Objective:To explore the growth performance of low-cost mobile hatchery produced silver barb (Barbodes gonionotus) fingerling comparing with typical one, field laboratory complex hatchery. Methods:Assigning Treatment I and Treatment II stoked with low-cost mobile hatchery and field laboratory complex hatchery originated fingerling of silver barb in plastic circular tank. Results:The average weight gain (g/day) of fish was 0.18 and 0.16 g, length gain (cm/d) 0.03 and 0.02, gross yield 0.09 and 0.08 kg/tank per month, net yield 0.03 and 0.01 kg/tank per month respectively in Treatment I and Treatment II. The growth of low-cost mobile hatchery produced silver barb was higher (P Conclusions:The fry from both origins showed more or less similar growth performance and none of them was superior. Considering the economic feasibility in both cases, it is assumed that the intensive culture of silver barb is possible at field level.

  7. Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing Bacterium Bacillus megaterium SP1 and Its Application in Aquaculture Wastewater Treatment

    Liang Luo


    Full Text Available A bioflocculant-producing bacterium, Bacillus megaterium SP1, was isolated from biofloc in pond water and identified by using both 16S rDNA sequencing analysis and a Biolog GEN III MicroStation System. The optimal carbon and nitrogen sources for Bacillus megaterium SP1 were 20 g L−1 of glucose and 0.5 g L−1 of beef extract at 30°C and pH 7. The bioflocculant produced by strain SP1 under optimal culture conditions was applied into aquaculture wastewater treatment. The removal rates of chemical oxygen demand (COD, total ammonia nitrogen (TAN, and suspended solids (SS in aquaculture wastewater reached 64, 63.61, and 83.8%, respectively. The volume of biofloc (FV increased from 4.93 to 25.97 mL L−1. The addition of Bacillus megaterium SP1 in aquaculture wastewater could effectively improve aquaculture water quality, promote the formation of biofloc, and then form an efficient and healthy aquaculture model based on biofloc technology.

  8. Use of microparticulated whey protein concentrate, exopolysaccharide-producing Streptococcus thermophilus, and adjunct cultures for making low-fat Italian Caciotta-type cheese.

    Di Cagno, R; De Pasquale, I; De Angelis, M; Buchin, S; Rizzello, C G; Gobbetti, M


    Low-fat Caciotta-type cheeses were manufactured with partially skim milk (fat content of ~0.3%) alone (LFC); with the supplementation of 0.5% (wt/vol) microparticulated whey protein concentrate (MWPC) (LFC-MWPC); with MWPC and exopolysaccharides (EPS)-producing Streptococcus thermophilus ST446 (LFC-MWPC-EPS); and with MWPC, EPS-producing strain ST446, and Lactobacillus plantarum LP and Lactobacillus rhamnosus LRA as adjunct cultures (LFC-MWPC-EPS-A). The non-EPS-producing isogenic variant Streptococcus thermophilus ST042 was used for making full-fat Caciotta-type cheese (FFC), LFC, and LFC-MWPC. Cheeses were characterized based on compositional, microbiological, biochemical, texture, volatile components (purge and trap, and solid-phase microextraction coupled with gas chromatography-mass spectrometry), and sensory analyses. Compared with FFC and LFC (51.6 ± 0.7 to 53.0 ± 0.9%), the other cheese variants retained higher levels of moisture (60.5 ± 1.1 to 67.5 ± 0.5%). The MWPC mainly contributed to moisture retention. Overall, all LFC had approximately one-fourth (22.6 ± 0.8%) of the fat of FFC. Hardness of cheeses slightly varied over 7d of ripening. Microbial EPS positively affected cheese texture, and the texture of LFC without MWPC or microbial EPS was excessively firm. Free amino acids were at the highest levels in LFC treatments (2,705.8 ± 122 to 3,070.4 ± 123 mg/kg) due to the addition of MWPC and the peptidase activity of adjunct cultures. Aldehydes, alcohols, ketones, sulfur compounds, and short- to medium-chain carboxylic acids differentiated LFC variants and FFC. The sensory attributes pleasant to taste, intensity of flavor, overall acceptability, and pleasant to chew variously described LFC-MWPC-EPS and LFC-MWPC-EPS-A. Based on the technology options used, low-fat Caciotta-type cheese (especially ripened for 14 d) has promising features to be further exploited as a suitable alternative to the full-fat variant.

  9. Agency, Context and Meaning

    Folkmann, Mads Nygaard


    history, 2) the question of context in and of design, i.e. which contexts give meaning to design; this question calls for interpretive models of cultural analysis of the circuit of design in acknowledging phases and aspects of production, mediation and consumption, and 3) the question of the meaning...... of meaning formulation and cultural contexts and, by this, contest design. In reflecting the foundational ground of design in terms of its agency, contexts and meaning constituents, design and its questioning of meaning can be critically reframed....


    Ancuta-Mihaela ZETIU


    Full Text Available This paper presents an analysis on the impact generated by hosting the International Festival of Education, 2013 edition, on Iasi’s image and its contribution in the competition for winning the title "European Capital of Culture 2021". The article reviews aspects related to culture, events and city branding, the strategy and the effects of hosting a cultural event on the host city. Cultural event was discussed as a tool used for urban regeneration and branding a tourist destination. Moreover, statistics show how the event contributed to increase tourism in Iasi.

  11. Culturing Protozoa.

    Stevenson, Paul


    Compares various nutrient media, growth conditions, and stock solutions used in culturing protozoa. A hay infusion in Chalkey's solution maintained at a stable temperature is recommended for producing the most dense and diverse cultures. (WB)

  12. Culturing Protozoa.

    Stevenson, Paul


    Compares various nutrient media, growth conditions, and stock solutions used in culturing protozoa. A hay infusion in Chalkey's solution maintained at a stable temperature is recommended for producing the most dense and diverse cultures. (WB)

  13. 南京地铁媒介与城市文化的空间生产%Nanjing Subway Media and the Produce of City Culture Space



    “地铁即为媒介”的观点既有麦克卢汉的理论支持,亦有史实例证。按照不同的感知维度,作为媒介的地铁空间可分为实体空间和虚拟空间两类。以南京地铁为例进行实证研究,可解读出地铁媒介空间的生产机制为:实体空间的分割、搬运、定义、承载以及虚拟空间的符号选择、表征、重组和议程设置。只有合理利用地铁媒介空间进行城市文化生产,才能充分发掘出地铁文化传播的潜力。%“Subway is the media”can be proved by the theory of Marshall Mcluhan and history events.Accord-ing to the different dimensions of the perceived, the space of media subway can be divided into physical space and virtual space.The empirical research of nanjing subway can explain the mechanisms for the produc-tion of space:the division,handling,definition,bearing of physical space and symbol selection,the charac-terization,restructuring and agenda setting of Virtual space.Only use the underground space legitimately on urban culture production,can subway culture communication be fully excavated.

  14. Local structures and electronic band states of α−Fe2O3 polycrystalline particles in the glazes of the HIZEN celadons produced in the Edo period of Japan, by means of X-ray absorption spectra (II)

    Hidaka, M; Ohashi, K.; R. P. Wijesundera; L. S. R. Kumara; Sugihara, S.; Momoshima,N.; Kubuki,S.; Sung,N. E.


    HIZEN celadon glazes produced in 1630's to 1790's (Edo period, Japan) have been investigated by means of X-ray absorption spectra (XAS) near a Fe-K edge by using synchrotron radiation and a Mössbauer spectrum. The XAS suggest that the local structure around Fe2O3 fine powders is slightly different between the Izumiyama ceramics of mainly the Quartz-SiO2 and Ohkawachi ceramics of mainly the feldspar of (K,Na)Si3O8 (Sanidine), and that the glazes of the HIZEN celadons include the Fe2O3 fine pow...

  15. 利用啤酒废水培养生物絮凝剂产生菌的研究%Study on the culture of bioflocculant producing bacteria by brewery wastewater

    芦艳; 乔福珍; 孟丽丽


    从空气和活性污泥中筛选出絮凝剂产生菌,对其进行筛选并纯化得到絮凝活性较高且稳定的菌株M3.以啤酒废水为廉价培养基,对絮凝剂产生菌M3进行培养,考察外加碳源、氮源、培养基pH值、培养时间等因素对絮凝剂产生菌絮凝效果的影响.得出了M3的最佳培养条件:直接利用啤酒废水,无需另外添加碳源和氮源,只需添加0.5%的KH_2PO_4,温度为30 ℃,培养基初始pH值为8.5,培养时间为48 h,摇床转速为160 r/min.在此条件下所产生的絮凝剂对高岭土悬液絮凝率高达93.5%.%Flocculant-producing bacteria was isolated mainly from the air and activated sludge, and strain M3,which exhibited excellent flocculation activity and was stable,were obtained by means of screening and purification.Brewery wastewater was used as low-cost medium to culture bacterium M3.The influence of factors of external carbon source,nitrogen source,medium pH value,culture time on strain flocculation effect was investigated and its culture conditions were optimized.The flocculating rate of kaolin suspension was up to 93.5% under the conditions as that the carbon and nitrogen sources was not needed to add,cultivating temperature 30 ℃,initial pH of culture medium 8.5,cultivating time 48 h and shaking speed 160 r/min.

  16. Isolation and purification of enniatins A, A(1), B, B(1), produced by Fusarium tricinctum in solid culture, and cytotoxicity effects on Caco-2 cells.

    Meca, G; Ruiz, M J; Soriano, J M; Ritieni, A; Moretti, A; Font, G; Mañes, J


    Enniatins (ENs) are antibiotic compounds of hexadepsipeptidic structure produced by several strains of Fusarium spp. The ENs A, A(1), B, B(1) were purified from extracts of Fusarium tricinctum grown on a solid medium of corn, by a low pressure liquid chromatography (LPLC) on reverse phase of Amberlite XAD-7 followed by semipreparative LC. The purity and the structure of the isolated compounds were confirmed by LC-MS/MS. The technique of the purification of the fungal extract enabled complete separation of the ENs A, A(1), B, B(1) with a mean purity of 97% for all the compounds. The cytoxicity of the ENs was tested in the cell lines of human origin (epithelial colorectal adenocarcinoma cells, Caco-2) by MTT assays. Only EN A(1) and B(1) evoked toxicity at the tested concentrations. The inhibitory concentration (IC(50)) for EN A(1) on Caco-2 cells was 12.3 microM, whereas the IC(50) produced by the EN B(1) was 19.5 microM. This study indicates that ENs, fungal metabolites that are commonly found in corn and in general in product composed by corn, may have a toxic potential for human health. Copyright 2010 Elsevier Ltd. All rights reserved.

  17. The formation of bases of culture of movement at children of 3–6 years old by means of rhythmic gymnastics

    Olga Riabchenko


    Full Text Available Purpose: to open the versatility of a concept of culture as a phenomena and as a process which course provides the preservation of the most significant viable social relations conformably to a concept of culture of movements of children of 3-6 years old. Material and Methods: the analysis of special scientific literature in which theoretical and practical aspects of the creation of physical culture are stated. Results: questions of the creation of physical training of children of 3–6 years old taking into account their individual physical developments are considered. The most important provisions are noted in the structure of physical culture which doesn’t allow solving this problem with a full scientific validity now. Conclusions: the basic provisions are allocated which decision is a paramount task for the creation of the evidence-based system of physical training of children of the advanced preschool age.

  18. Atividade alelopática do filtrado de cultura produzido por Fusarium solani Allelopathic activity of culture filtrate produced by Fusarium solani

    A.P.S. Souza Filho


    germination and radicle and hypocotyl elongation of the weeds Mimosa pudica and Senna obtusifolia. The results showed potential inhibitory allelopathic activity of the Fusarium culture filtrate, varying according to concentration and receiving plants. The intensity of the inhibition effects promoted by the extracts was clearly associated to concentration, with the major effect being observed at 4%. Regardless of concentration and bioassays, Mimosa pudica was more sensitive to the toxin effects of the culture filtrate. Radicle elongation was more intensely inhibited by the culture filtrate toxins. The results showed potential for the use of the toxins produced by Fusarium solani f. sp. pipers, as an alternative source to control weeds. However, further studies should be carried out.

  19. Transcriptome and proteome analysis of antibody-producing mouse myeloma NS0 cells cultivated at different cell densities in perfusion culture.

    Krampe, Britta; Swiderek, Halina; Al-Rubeai, Mohamed


    A combined gene and protein expression profiling was performed to gain a deeper insight into the intracellular response of the antibody-producing GS-NS0 cell line in continuous perfusion culture. Growth rate, production rate, metabolic activity and viability declined with increasing cell density, dilution rate and time. Transcriptome and proteome analyses of cells at three different densities revealed 53 genes and 47 proteins as having significantly altered expression levels at HCD (high cell density). The results showed an increased up-regulation of genes/proteins involved in cellular energy production with increasing cell density. Furthermore, the intensified process triggered a cellular response to external stress stimuli, revealed by an overexpression of the genes/proteins implicated in cell-cycle arrest [e.g. Rb1 (retinoblastoma 1 gene) and Cdkn1b (cyclin-dependent kinase inhibitor 1B gene)] and in the induction of pro-apoptotic genes/proteins [e.g. Tnfrsf (tumour necrosis factor receptor superfamily gene), Nfkappa bia (gene coding for nuclear factor-kappaB inhibitor), HSP60 (heat-shock protein of molecular mass 60 kDa) and heterogeneous nuclear ribonucleoprotein K]. Interestingly, we observed a down-regulation of the transcription factor interferon regulatory factor 4 involved in the unfolded-protein-response process and protein disulfide-isomerase family members responsible for protein folding and assembly. Additionally, subunits of proteasome complex were highly expressed at HCD. Microarray, real-time quantitative reverse-transcription PCR and Western-blot analyses demonstrated a consistent trend of decrease in IgG heavy-chain level with increasing cell density. HSP60, which inhibits apoptosis by complexing with pro-apoptotic proteins such as Bax and Bak, was repressed at HCD. Overall, the data suggested that the balance among several factors involved in energy metabolism might be essential for fine-tuning the cell choice between survival and apoptosis

  20. Review on the Meaning of Cultural Industry in Western Academia%西方学术界文化产业含义的研究述评



    The western scholars have different cognitions of the connotation and the definition of denotation of the concept of cultural industry, but they reach a consensus on the core issues. Generally speaking, the concept of western culture industry mainly refers to provide the community with the activities of cultural products and services. When the westem scholars give a definition of cultural industry, they emphasize the internal relationship, creative features, social significance and spiritual value of cultural industry.%西方学者对文化产业概念内涵和外延的界定存在着较大的差别,但在一些核心问题上他们的观点又是一致的。大体说来,西方文化产业的概念主要是指为社会提供文化产品和服务的活动的集合。西方学者在界定文化产业的概念时,注重强调文化产业内部的联系、产业的创意特征及产业的社会意义和精神价值。

  1. Probing into the Meaning of Peace in African Traditional Religion and Culture%非洲传统宗教文化中的和平含义



    This paper intends to examine the meaning of peace in African tradi-tional religion (ATR) and culture, The essential features of ATR under three head- ings, which may be regarded as the three principal dimensions of religion: belief, worship and morality. Considering Africa as a whole, the main objects of traditional religious belief are: God, the divinities, spirits and the ancestors. Religion for the Africans embraces life as a whole and worship touches every aspect of their lives and this is done through sacrifices, offerings, prayers, invocation, praises, music and dance. The practical aspect of belief in ATR is not only worship but also human con- duct. Belief in God and in the other spiritual beings implies a certain type of con- duct, conduct that respects the order established by God and watched over by the divinities and the ancestors. So the social morality is based on these. Two things can therefore be said of the traditional world - view of the Africans, namely, that it is permeated by the spirit and that it is anthropocentric. In traditional African societies, peace is not an abstract poetic concept, but rather a down -to -earth and practical concept. In ATR peace is conceived not in relation to conflict and war, but in rela- tion to order, harmony and equilibrium. It is a religious value in that the order, har- mony and equilibrium in the universe and society is believed to be divinely estab- lished and the obligation to maintain them is religious. It is also a moral value since good conduct is required of human beings if the order, harmony and equilibrium are to be maintained. Finally, by way of conclusion possibility of world peace from the perspective o f we shall take a forward look at the ATR.%非洲传统宗教的基本特征体现在信仰、崇拜、道德和世界观四方面。就非洲整体而言,传统宗教信仰的主要对象是上帝、神、精灵和祖先;崇拜涉及生活的各个方面,通过献祭、供品、祷告、乞

  2. Evaluation of lipolysis and volatile compounds produced by three Penicillium roqueforti commercial cultures in a blue-type cheese made from ovine milk

    E. Salvatore


    Full Text Available The aim of this work was to compare the effect of three different Penicillium roqueforti commercial cultures (named PS1, PS2 and PS3 on proteolysis, lipolysis and volatile flavour profile of a blue cheese made from ovine milk and lamb paste rennet. Proteolytic parameters were not significantly affected by the Penicillium roqueforti culture, while cheeses manufactured using PS2 and PS3 cultures showed the higher amount of free fatty acids (FFA and volatile FFA when compared with PS1 culture after 30 days of ripening. This study can provide important information for obtainingthe desired extent of lipolysis in this type of blue cheese.

  3. A Simplified Method for Three-Dimensional (3-D Ovarian Tissue Culture Yielding Oocytes Competent to Produce Full-Term Offspring in Mice.

    Carolyn M Higuchi

    Full Text Available In vitro growth of follicles is a promising technology to generate large quantities of competent oocytes from immature follicles and could expand the potential of assisted reproductive technologies (ART. Isolated follicle culture is currently the primary method used to develop and mature follicles in vitro. However, this procedure typically requires complicated, time-consuming procedures, as well as destruction of the normal ovarian microenvironment. Here we describe a simplified 3-D ovarian culture system that can be used to mature multilayered secondary follicles into antral follicles, generating developmentally competent oocytes in vitro. Ovaries recovered from mice at 14 days of age were cut into 8 pieces and placed onto a thick Matrigel drop (3-D culture for 10 days of culture. As a control, ovarian pieces were cultured on a membrane filter without any Matrigel drop (Membrane culture. We also evaluated the effect of activin A treatment on follicle growth within the ovarian pieces with or without Matrigel support. Thus we tested four different culture conditions: C (Membrane/activin-, A (Membrane/activin+, M (Matrigel/activin-, and M+A (Matrigel/activin+. We found that the cultured follicles and oocytes steadily increased in size regardless of the culture condition used. However, antral cavity formation occurred only in the follicles grown in the 3-D culture system (M, M+A. Following ovarian tissue culture, full-grown GV oocytes were isolated from the larger follicles to evaluate their developmental competence by subjecting them to in vitro maturation (IVM and in vitro fertilization (IVF. Maturation and fertilization rates were higher using oocytes grown in 3-D culture (M, M+A than with those grown in membrane culture (C, A. In particular, activin A treatment further improved 3-D culture (M+A success. Following IVF, two-cell embryos were transferred to recipients to generate full-term offspring. In summary, this simple and easy 3-D ovarian

  4. Analysis The Meaning And Significance Of The Cultural Construction In The College Dormitory%探析高校宿舍文化建设的内涵和意义

    李海兆; 孙晓明; 翟杰


    The dormitory culture is an important part of the Campus Culture .It can guide students set up their philos-ophy of life .This article is based on the cultural construction of the university campus dormitory ,explore the cultural connotation and characteristics of the quarters ,analysis the basic principles of the college dormitory cultural construction , probe into set up a way to build the dormitory culture ,and all the efforts should be settled in the meanings and values of high school dorm calture development .%高校校园文化的含义非常广泛,宿舍文化是其中的一个重要组成部分,而且它也能够引导大学生的世界观人生观价值观的培养。论文立足高校校园宿舍文化建设,探索宿舍文化的内涵以及特点,探析高校宿舍文化建设的基本原则,剖析宿舍文化创建的途径,并把建设高校宿舍文化的意义与价值作为研究的最终落脚点。

  5. A Comparative Analysis on the Cultural Meaning of Ani-mals in China and Russia%中俄民族动物文化涵义对比分析



    In the cultures of different nations, animals have infil-trated into various fields of social life. This thesis will make a comparison of the connotation of animal culture between Chinese and Russian with an aim to explore the differences and similari-ties and to reveal the hidden cultural meanings of animals in the cultures of the two countries, so as to offer readers a further un-derstanding of the culture of Russia, and enhance the readers' a-bility of Chinese-Russian inter-cultural communication.%在各民族文化中,动物渗透到了社会生活的各个领域。本文即从动物文化涵义的角度出发,对汉、俄语中体现的两国动物文化的涵义进行比较分析,审视中俄民俗的部分现象,探索两国动物文化涵义的异同,揭示动物在两国文化中隐藏的文化信息,从而更加深入了解俄罗斯语言和文化,以提高中俄文化语言的交际能力。

  6. The Research about the Cultural Industry Producers' Construction in Network Market---Take Suqian City for Example%文化产业网络交易市场生产主体建设的研究--以宿迁市为例

    周浩; 唐传涛


    In a certain means, the development of cultural industry network market, depends on the construction of the culture industry producers. Market, competition, supply and demand effect each other. Interactional internal connection and restrictive function constitute the main contents of the cultural industry market. It is necessary for Suqian to construct network culture industry market. Cultural industry network market need to combine advantage cultural resources, advantage industry and advantage scientific and technological innovation in Suqian. It is important to stimulate the creativity of cultural production talent and actively cultivate the cultural production of leading en-terprises.%文化产业网络交易市场的推进和发展,在很大程度上,取决于文化产业生产主体队伍的建设。市场、供求、工资、竞争等要素对生产主体的影响并不是孤立的,他们互为因果,相互制约的内在联系和制约作用构成了文化产业交易市场生产主体建设的主要内容。宿迁建设文化产业网络交易市场生产主体,要高起点、科学规划,准确定位,发展区域文化产业网络交易市场,要结合文化资源优势、行业优势和科技创新优势,理顺体制,激发文化生产人才的创造活力,积极培育文化生产龙头企业。

  7. Kinetics of early in vitro development of bovine in vivo- and in vitro-derived zygotes produced and/or cultured in chemically defined or serum-containing media

    Holm, P; Booth, P J; Callesen, H


    The kinetics of the in vitro development of early embryos from bovine zygotes derived in vitro and in vitro were compared, investigating the effect of serum during in vitro maturation and fertilization (IVM-IVF) and in culture. Zygotes were collected from superovulated heifers or produced in vitro...

  8. Fermentative capabilities and volatile compounds produced by Kloeckera/Hanseniaspora and Saccharomyces yeast strains in pure and mixed cultures during Agave tequilana juice fermentation.

    González-Robles, Ivonne Wendolyne; Estarrón-Espinosa, Mirna; Díaz-Montaño, Dulce María


    The fermentative and aromatic capabilities of Kloeckera africana/Hanseniaspora vineae K1, K. apiculata/H. uvarum K2, and Saccharomyces cerevisiae S1 and S2 were studied in pure and mixed culture fermentations using Agave tequila juice as the culture medium. In pure and mixed cultures, Kloeckera/Hanseniaspora strains showed limited growth and sugar consumption, as well as low ethanol yield and productivity, compared to S. cerevisiae, which yielded more biomass, ethanol and viable cell concentrations. In pure and mixed cultures, S. cerevisiae presented a similar behaviour reaching high biomass production, completely consuming the sugar, leading to high ethanol production. Furthermore, the presence of S. cerevisiae strains in the mixed cultures promoted the production of higher alcohols, acetaldehyde and ethyl esters, whereas Kloeckera/Hanseniaspora strains stimulated the production of ethyl acetate and 2-phenyl ethyl acetate compounds.

  9. Kinetics of early in vitro development of bovine in vivo- and in vitro-derived zygotes produced and/or cultured in chemically defined or serum-containing media

    Holm, P; Booth, P J; Callesen, H


    at first cleavage (defined serum: 20-22% versus in vivo-derived: 8 P cell cycles of in vivo-derived zygotes were 1-5 h shorter compared with those of in vitro-derived zygotes cultured under similar conditions (P ... development was affected by serum during IVM-IVF and culture. The first and fourth cell cycles were prolonged by 4-5 h in the absence of serum during IVM-IVF, whereas the presence of serum during culture decreased the duration of the fourth cell cycle and triggered premature blastulation. The results......The kinetics of the in vitro development of early embryos from bovine zygotes derived in vitro and in vitro were compared, investigating the effect of serum during in vitro maturation and fertilization (IVM-IVF) and in culture. Zygotes were collected from superovulated heifers or produced in vitro...

  10. Comparative study of predatory responses in blue mussels (Mytilus edulis L.) produced in suspended long line cultures or collected from natural bottom mussel beds

    Christensen, Helle Torp; Dolmer, Per; Petersen, Jens Kjerulf


    Blue mussels (Mytilus edulis L.) are a valuable resource for commercial shellfish production and may also have uses as a tool in habitat improvement, because mussel beds can increase habitat diversity and complexity. A prerequisite for both commercial mussel production and habitat improvement...... is the availability of seed mussels collected with minimum impact on the benthic ecosystem. To examine whether mussels collected in suspended cultures can be used for bottom culture production and as tool in habitat improvement, the differences in predatory defence responses between suspended and bottom mussels...... originated from suspended cultures had a higher length increment and lower mortality when compared to bottom mussels. It is concluded that suspended mussels potentially are an alternative resource to bottom culture and can be used in habitat improvement of mussel beds, but that the use of suspended mussels...

  11. Treatment of produced water by means of a new technology (MDIF): application for waters containing oil at low concentrations; Tratamento de aguas produzidas por meio de nova tecnologia (MDIF): aplicacao para aguas contendo baixas concentracoes em oleo

    Fernandes Junior, Wilaci Eutropio [PETROBRAS S.A., Mossoro, RN (Brazil). Unidade de Negocio de Exploracao e Producao. Ativo de Producao Mossoro]. E-mail:; Paulo, Joao Bosco de Araujo; Moraes, Norberto Araujo de Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Programa de Pos-graduacao em Engenharia Quimica). E-mails:;; Lima, Antonio Faria; Lacerda, Geraldo de Moura [PETROBRAS S.A., Natal, RN (Brazil). Unidade de Negocio de Exploracao e Producao. Gerencia de Engenharia de Instalacoes, Processamento e Automacao]. E-mails:;


    The production of oil is associated with waters which exist naturally at the reservoirs or were injected into wells. These waters, named produced waters, contain beside free oil, emulsioned or micro-emulsioned oil which can not be discharged directly at the environment. The conventional decanters are not efficient to separate this kind of oil dispersed as fine droplets. In this case, a promising alternative to solve the problem of oil/water separation is accomplished by means of the phase inversion method. This method is the basis of working of a new model of mixer-settler which has a vertical disposition and occupies a small surface area. The last characteristic becomes especially important when exist a limitation of place, for example, over the maritime platforms to explore oil. The device of laboratory of the equipment named MDIF (mixer-settler based on phase inversion) has been efficient for treat waters containing up to 2 000 mg/L of emulsioned oil. This equipment on a semi-industrial scale was installed in the entrance of oil/water separator (OWS) from effluent treatment plant from Guamare, Rio Grande do Norte (ETP/GMR) and treats produced waters contaminated with oil at low concentrations (ranging from 30 to 150 mg/L) and throughputs of 320 m3/d (47,4 m3 m-2 h-1). Good results were obtained on oil/water separation which leads to the necessary specification to discharge waters. Besides, the non dependence of the efficiency of separation in the face of the salinity of the medium becomes the equipment a new technology to treat wastewaters containing oil at low concentrations. In this condition conventional equipment do not present a good efficiency of separation, till dispersed droplets are very small (less than 100 {mu}m) requiring a long time of sedimentation. (author)

  12. A paternidade na contemporaneidade: um estudo de mídia sob a perspectiva dos Estudos Culturais The meanings of fatherhood in the media: a Cultural Studies perspective

    Inês Hennigen


    Full Text Available Neste artigo discutimos as possibilidades que a perspectiva dos Estudos Culturais abre para a pesquisa em Psicologia Social e, de forma mais específica, para a temática da paternidade. Questões relativas à construção das identidades e à importância da mídia neste processo são discutidas. A paternidade é aqui concebida como uma construção social que acompanha o caráter flexível das demais posições identitárias. A análise de um comercial de televisão é apresentada e mostra como diferentes aspectos da cultura contemporânea se atravessam e mesclam com a temática pai.In this article, we discuss the possibilities that the perspective of Cultural Studies open for the research in Social Psychology, more specifically, for the paternity´s thematic. Questions related to the identities construction and the media importance in this process are discussed. Paternity here is conceived as a social construction that follows the flexible character of other identity positions. The analysis of a television advertising is presented and shows how different aspects of the contemporary culture cross over and mix with the father´s thematic.

  13. Culture versus the law in the decision not to vaccinate children: meanings assigned by middle-class couples in São Paulo, Brazil.

    Barbieri, Carolina Luisa Alves; Couto, Márcia Thereza; Aith, Fernando Mussa Abujamra


    This study aimed to learn how middle-class parents in the city of São Paulo, Brazil, interpreted the country's prevailing vaccination requirements, based on their experiences with vaccinating, selectively vaccinating, or not vaccinating their children. A qualitative approach was used with in-depth interviews. The analytical process was guided by content analysis and the theoretical framework of the anthropology of the law and morality. For parents that vaccinated, Brazil's culture of immunization outweighed the feeling of compliance with the law; for selective parents, selection of vaccines was not perceived as deviating from the law. In both, the act of vaccinating their children was a matter of moral status. Meanwhile, the non-vaccinators, counter to the legal perspective, attributed their choice to care for the child on grounds that mandatory vaccination was contrary to their way of life; they experienced a feeling of social coercion and fear of legal impositions. Vaccination is an important practice in public health, but it can reveal tensions and conflicts from normative systems, whether moral, cultural, or legal.

  14. Conditioned Attraction, Similarity, and Evaluative Meaning. Language, Personality, Social, and Cross-Cultural Study and Measurement of the Human A-R-D (Motivational) System.

    Stalling, Richard B.

    Recent stimulus-response formulations have indicated that similarity between persons functions as an unconditioned stimulus (UCS) and that interpersonal attraction is a classically conditioned evaluative response. The thesis of this study is that similarity is a correlate of evaluative meaning and that the latter rather than the former is…

  15. The Protective Function of Meaning of Life on Life Satisfaction among Chinese Students in Australia and Hong Kong: A Cross-Cultural Comparative Study

    Pan, Jia-Yan; Wong, Daniel Fu Keung; Joubert, Lynette; Chan, Cecilia Lai Wan


    Objective: The authors compared the predictive effects of acculturative stressors and meaning of life on life satisfaction between Chinese students in Australia and in Hong Kong. Participants: In 2006, the researchers recruited 606 Chinese students studying abroad at the University of Melbourne in Australia and at 6 universities in Hong Kong.…

  16. Use of tissue culture techniques for producing virus-free plant in garlic and their identification through real-time PCR.

    Taşkın, Hatıra; Baktemur, Gökhan; Kurul, Mehmet; Büyükalaca, Saadet


    This study was performed for comparison of meristem culture technique with shoot tip culture technique for obtaining virus-free plant, comparison of micropropagation success of two different nutrient media, and determination of effectiveness of real-time PCR assay for the detection of viruses. Two different garlic species (Allium sativum and Allium tuncelianum) and two different nutrient media were used in this experiment. Results showed that Medium 2 was more successful compared to Medium 1 for both A. tuncelianum and A. sativum (Kastamonu garlic clone). In vitro plants obtained via meristem and shoot tip cultures were tested for determination of onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV) through real-time PCR assay. In garlic plants propagated via meristem culture, we could not detect any virus. OYDV and LYSV viruses were detected in plants obtained via shoot tip culture. OYDV virus was observed in amount of 80% and 73% of tested plants for A. tuncelianum and A. sativum, respectively. LYSV virus was found in amount of 67% of tested plants of A. tuncelianum and in amount of 87% of tested plants of A. sativum in this study.

  17. Use of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR

    Hatıra Taşkın


    Full Text Available This study was performed for comparison of meristem culture technique with shoot tip culture technique for obtaining virus-free plant, comparison of micropropagation success of two different nutrient media, and determination of effectiveness of real-time PCR assay for the detection of viruses. Two different garlic species (Allium sativum and Allium tuncelianum and two different nutrient media were used in this experiment. Results showed that Medium 2 was more successful compared to Medium 1 for both A. tuncelianum and A. sativum (Kastamonu garlic clone. In vitro plants obtained via meristem and shoot tip cultures were tested for determination of onion yellow dwarf virus (OYDV and leek yellow stripe virus (LYSV through real-time PCR assay. In garlic plants propagated via meristem culture, we could not detect any virus. OYDV and LYSV viruses were detected in plants obtained via shoot tip culture. OYDV virus was observed in amount of 80% and 73% of tested plants for A. tuncelianum and A. sativum, respectively. LYSV virus was found in amount of 67% of tested plants of A. tuncelianum and in amount of 87% of tested plants of A. sativum in this study.

  18. Isolation of TDA-producing Phaeobacter strains from sea bass larval rearing units and their probiotic effect against pathogenic Vibrio spp. in Artemia cultures

    Grotkjær, Torben; Bentzon-Tilia, Mikkel; D'Alvise, Paul;


    -DNA hybridization indicated that they could belong to a new species. The genomes contained genes involved in synthesis of the antibacterial compound tropodithietic acid (TDA), and its production was confirmed by UHPLC-TOFMS. The new Phaeobacter colonized live feed (Artemia) cultures and reduced Vibrio counts...... significantly, since they reached only 104 CFUmL-1, as opposed to 108 CFUmL-1 in non-Phaeobacter treated controls. Survival of V. anguillarum-challenged Artemia nauplii was enhanced by the presence of wild type Phaeobacter compared to challenged control cultures (89±1.0% vs 8±3.2%). In conclusion, TDA...

  19. Exploration of the hydrogen producing potential of Rhodobacter capsulatus chemostat cultures: The application of deceleration-stat and gradient-stat methodology

    Hoekema, S.; Breukelen, van F.R.; Janssen, M.G.J.; Tramper, J.; Wijffels, R.H.


    In this work, the dependency of the volumetric hydrogen production rate of ammonium-limited Rhodobacter capsulatus chemostat cultures on their imposed biomass concentration and dilution rate was investigated. A deceleration-stat experiment was performed by lowering the dilution rate from 1.0 d-1 to

  20. Exploration of the hydrogen producing potential of Rhodobacter capsulatus chemostat cultures: The application of deceleration-stat and gradient-stat methodology

    Hoekema, S.; Breukelen, van F.R.; Janssen, M.G.J.; Tramper, J.; Wijffels, R.H.


    In this work, the dependency of the volumetric hydrogen production rate of ammonium-limited Rhodobacter capsulatus chemostat cultures on their imposed biomass concentration and dilution rate was investigated. A deceleration-stat experiment was performed by lowering the dilution rate from 1.0 d-1 to

  1. Analysis of energy culture the Crambe (Crambe abyssinica Hochst) produced in no tillage; Analise energetica da cultura do Crambe (Crambe abyssinica Hochst) produzida em plantio direto

    Jasper, Samir Paulo; Biaggioni, Marco Antonio Martin; Silva, Paulo Roberto Arbex; Seki, Andre Satoshi; Bueno, Osmar Carvalho de [Universidade Estadual Paulista (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas], E-mail:


    The objective of this study was to characterize the energy consumption in the deployment and conduct of the culture of Crambe abyssinica Hochst in no-tillage, at the Experimental Farm Farm belonging to the Faculty of Agronomic Sciences - UNESP, located in Botucatu - SP. Assessing the sustainability of the system were estimated, energy efficiency and culture of crambe. To survey the components of the structure of expenditures, we used coefficients Energetic in specialized literature. The structures of energy expenditure by type, source and form, demonstrated that the indirect energy participated with 65.03%, industrial sources, fossil and biological accounted for 68.80%, 23.67% and 11.30%, respectively. The forms have been introduced; chemical fertilizers contributed 44.31% and diesel to 23.04% to 67.35% of energy expenditure, showing the dependence of the system of energy sources and industrial fuels. The efficiency of the culture was found to be 9.98, indicating that for each calorie used in the production of crambe return was 8.98 units, the efficiency was above 38, demonstrating the high sustainability of the agricultural system to reach energy cultural exceeding 37 thousand MJ ha{sup -1}. (author)

  2. Use of a genetically enhanced, pediocin-producing starter culture, Lactococcus lactis subsp. lactis MM217, to control Listeria monocytogenes in cheddar cheese

    Buyong, N; Kok, J; Luchansky, JB


    Cheddar cheese was prepared with Lactococcus lactis subsp, lactis MM217, a starter culture which contains pMC117 coding for pediocin PA-1, About 75 liters of pasteurized milk (containing ca, 3.6% fat) was inoculated with strain MM217 (ca, 10(6) CFU per ml) and a mixture of three Listeria monocytogen

  3. Organizing and conducting career guidance and crime prevention among young people by means of physical culture and sports specialized university departments Internal Affairs of Ukraine.

    Zakorko I.P


    Full Text Available At the present stage of social development takes on special urgency the problem of finding new means and methods of crime prevention among youth in the context of strengthening the nation's health. In our opinion, employees of the Ukrainian Interior Ministry must take a decisive role in solving these problems. One of the most effective ways to solve them is to organize youth sports schools and clubs to professionally-applied sports at the bases of relevant departments of higher educational institutions of Ministry of Internal Affairs of Ukraine, the involvement of the best specialists and trainers.

  4. What Does the Mean Mean?

    Watier, Nicholas N.; Lamontagne, Claude; Chartier, Sylvain


    The arithmetic mean is a fundamental statistical concept. Unfortunately, social science students rarely develop an intuitive understanding of the mean and rely on the formula to describe or define it. According to constructivist pedagogy, educators that have access to a variety of conceptualizations of a particular concept are better equipped to…

  5. The work of cultural transition

    Ryba, T. V.; Stambulova, N. B.; Ronkainen, Noora J.


    In today's uncertain, fluid job market, transnational mobility has intensified. Though the concept of cultural transition is increasingly used in sport and career research, insight into the processes of how individuals produce their own development through work and relationships in shifting...... cultural patterns of meaning remains limited. The transnational industry of sports, in which athletes' psychological adjustment to cultural transitions has implications for both performance and meaningful life, serves as a backdrop for this article. This study applied the life story method to interviews...

  6. Default Meanings

    K. M. Jaszczolt


    In this paper I present some aspects of the controversy surrounding the semantics/pragmatics boundary and offer a possible solution in the form of Default Semantics. First, I present a brief introduction to Paul Grice's approach to meaning as intentional communication. Next, I address the problem of the unit of which truth conditions should be predicated, assuming after Grice that the most successful approach to meaning has to make use of truth conditions.Further, I extend the discussion to some aspects of neo-Gricean pragmatics, most notably the issue of pragmatic processing that contributes to the truth-conditional representation and the proposed shortcuts through this processing in the form of default meanings. In the main part, I present the theory of Default Semantics ( Jaszczolt 2005a) , assess some arguments in favour of such an interactive theory of meaning that combines information from various sources, and address the issue of compositionality of meaning so conceived. In the final part, I apply the theory of Default Semantics to expressions with future-time reference in English in order to demonstrate the advantages of a framework that combines various types of information about utterance meaning in one, merged semantic representation. The main advantage turns out to be, in this domain, the unified treatment of all expressions with future-time reference.

  7. Intonational meaning.

    Prieto, Pilar


    Traditionally, prosodic studies have focused on the study of intonational form and the study of intonational meaning has been relatively neglected. Similarly, the fields of semantics and pragmatics have paid little attention to the pragmatic uses of intonation. As a result, there is no firm agreement within the linguistic community on how to integrate the analysis of intonational meaning across languages into a unified prosodic, semantic, and pragmatic approach. This article provides an overview of the literature on intonational meaning, describing the recent advances made in the fields of prosody, semantics/pragmatics, and syntax. Several theoretical approaches to explaining the semantics and pragmatics of intonation are presented. A common feature to most frameworks is that intonation (1) should be regarded as an integral part of linguistic grammar; and (2) typically encodes meanings related to the modal aspect of propositions. However, features such as compositionality, duality of structure, and context-dependency are still hotly debated issues. These features will be discussed from different theoretical perspectives, and we will identify potential advances related to the full integration of intonational meaning into dynamic and multidimensional models of meaning.

  8. On the Folk Culture Basic of Dingguang Buddha Belief and Its Real Meaning%定光佛信仰的民间文化基础及其现实意义



    定光佛信仰是佛教与道教、民间信仰融合的典型个案;是客家文化形成和传播研究的重要内容。研究定光佛信仰在提炼传统文化精华、促进现代人性反思和人文科学建设等方面有重要的现实意义。%Dingguang Buddha Belief is a typical case of putting Buddhism, Taoism and folk belief together. It is the important content between the Hakka culture forming and dissemination research. Research on Dingguang Buddha Belief has important and real meaning in absorbing the traditional culture cream, promoting the reflection of modem human nature and humanistic science construction.

  9. Rapid detection of carbapenemase-producing Klebsiella pneumoniae strains derived from blood cultures by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).

    Sakarikou, Christina; Ciotti, Marco; Dolfa, Camilla; Angeletti, Silvia; Favalli, Cartesio


    Carbapenemase-producing Enterobacteriaceae (CPE), particularly carbapenemase-producing Klebsiella pneumoniae isolates, are important causative agents of nosocomial infections associated with significant mortality rates mostly in critical wards. The rapid detection and typing of these strains is critical either for surveillance purposes and to prevent outbreaks and optimize antibiotic therapy. In this study, the MALDI-TOF MS method was used to detect rapidly these isolates from blood cultures (BCs) and to obtain proteomic profiles enable to discriminate between carbapenemase-producing and non-carbapenemase-producing strains. Fifty-five K. pneumoniae strains were tested. Identification and carbapenemase-production detection assay using Ertapenem were performed both from bacterial pellets extracted directly from BCs flasks and from subcultures of these strains. For all isolates, a complete antimicrobial susceptibility testing and a genotypic characterization were performed. We found 100% agreement between the carbapenemase-producing profile generated by MALDI TOF MS and that obtained using conventional methods. The assay detected and discriminated different carbapenemase-producing K. pneumoniae isolates within 30 min to 3 h after incubation with Ertapenem. MALDI-TOF MS is a promising, rapid and economical method for the detection of carbapenemase-producing K. pneumoniae strains that could be successfully introduced into the routine diagnostic workflow of clinical microbiology laboratories.

  10. Contextual Meaning and Word Meaning

    Istvan Kecskes


    The paper has three goals: 1 ) Explain the role of context and word in meaning construction and comprehension. 2) Present a model that can explain meaning generated by both monolingual and multilingual meaning construction systems. 3) Discuss how the model can be applied to explain major issues in pragmatics.Pragmatics is understood here in a narrow sense as defined by Sperber & Noveck: "...pragmatics is the study of how linguistic properties and contextual factors interact in the interpretation of utterances" ( Sperber & Noveck 2004:1 ). It is argued that world knowledge is available to interlocutors in two forms: as encapsulated in lexical items based on prior encounters and experience (conventionalized cognitive context), and as provided by the actual situational context framed by the situation in which the interaction takes place. Meaning formally expressed in the linguistic interactional context is created on-the-spot, and is the result of the interaction of the two sides of world knowledge and the actual situational context.The paper makes three claims: First, supremacy of context is not unconditional in language processing. Second,salient meaning rather than literal meaning of lexical units plays a central role in comprehension. Third, intercultural communication differs from intracultural communication in the conceptual content of conventionalized cognitive contexts rather than in communicative means.

  11. callus culture


    and KH2PO4 -NH4NO3 depletion was fatal to grape cells and resulted in formation ... Individual phenolic compounds found in the grape cell cultures were ...... Characterization of in vitro anthocyanin-producing sour cherry ... Process Biochem.

  12. Antimicrobial peptides (AMPs) produced by Saccharomyces cerevisiae induce alterations in the intracellular pH, membrane permeability and culturability of Hanseniaspora guilliermondii cells

    Branco, Patrícia; Monteiro Lomba Viana, Tiago; Albergaria, Helena


    Hi results showed that after 24h, 23% of the AMP-treated cells were sub-lethally injured (with 0pHindicated that this subpopulation was under a viable but non-culturable (VBNC) state, which was further confirmed by recuperation assays. In summary, our study reveals...... on sensitive H. guilliermondii cells were evaluated in terms of intracellular pH (pHi), membrane permeability and culturability. Membrane permeability was evaluated by staining cells with propidium iodide (PI), pHi was determined by a fluorescence ratio imaging microscopy (FRIM) technique and culturability...... by a classical plating method. Results showed that the average pHi of H. guilliermondii cells dropped from 6.5 (healthy cells) to 5.4 (damaged cells) after 20min of exposure to inhibitory concentrations of AMPs, and after 24h 77.0% of the cells completely lost their pH gradient (∆pH=pHi-pHext). After 24h...

  13. O processo de cuidar da pessoa que sofreu queimaduras: significado cultural atribuído por familiares El proceso de cuidar de personas que sufrieron quemadura: significado cultural atribuido por familiares The process of delivering care to a burned person: cultural meaning attributed by relatives

    Lídia Aparecida Rossi


    Full Text Available Os objetivos deste estudo etnográfico foram identificar o significado cultural atribuído por familiares à participação no processo de cuidar da pessoa ultima de queimadura; identificar as estratégias de cuidado que os familiares focalizam frente à participação no cuidado. Os dados foram coletados mediante entrevistas semi-estruturadas com 25 familiares de clientes que sofreram queimaduras, admitidos em uma Unidade de Queimados e observações participantes. Os resultados mostram que o significado cultural atribuído por familiares de pessoas vítimas queimaduras ao processo de cuidar tem duas dimensões: a física e a moral e gera principalmente medo.Los objetivos fueron investigar los significados culturales atribuidos por familiares a la participación en el proceso de cuidar de personas víctimas de quemadura; identificar las estratégias que los familiares enfocan en su participación en el cuidado. Los datos fueron colectados a través de entrevistas semi-estructuradas con 25 familiares de clientes quemados admitidos en una Unidad de Quemados y a través de observaciones participantes realizadas durante el período de visitas y en reuniones con familiares de clientes. Los resultados de este estudio muestran que la perspectiva de cuidar de una persona que sufrió una quemadura tiene dos dimensiones: la física y la moral, y genera principalmente miedo.This study aimed at investigating the cultural meanings attributed by relatives to their participation in the process of delivering care to burned people; identifying the care strategies focused on by relatives during their participation in the care process. Data were collected by means of semi-structured interviews with 25 relatives of burned patients admitted in a Unit for Burned and by participant observation. The results of this study show that the perspective of giving care to a burned person has two dimensions: a physical dimension and a moral one; and generates mainly fear.

  14. Co-culture of neural crest stem cells (NCSC and insulin producing beta-TC6 cells results in cadherin junctions and protection against cytokine-induced beta-cell death.

    Anongnad Ngamjariyawat

    Full Text Available PURPOSE: Transplantation of pancreatic islets to Type 1 diabetes patients is hampered by inflammatory reactions at the transplantation site leading to dysfunction and death of insulin producing beta-cells. Recently we have shown that co-transplantation of neural crest stem cells (NCSCs together with the islet cells improves transplantation outcome. The aim of the present investigation was to describe in vitro interactions between NCSCs and insulin producing beta-TC6 cells that may mediate protection against cytokine-induced beta-cell death. PROCEDURES: Beta-TC6 and NCSC cells were cultured either alone or together, and either with or without cell culture inserts. The cultures were then exposed to the pro-inflammatory cytokines IL-1β and IFN-γ for 48 hours followed by analysis of cell death rates (flow cytometry, nitrite production (Griess reagent, protein localization (immunofluorescence and protein phosphorylation (flow cytometry. RESULTS: We observed that beta-TC6 cells co-cultured with NCSCs were protected against cytokine-induced cell death, but not when separated by cell culture inserts. This occurred in parallel with (i augmented production of nitrite from beta-TC6 cells, indicating that increased cell survival allows a sustained production of nitric oxide; (ii NCSC-derived laminin production; (iii decreased phospho-FAK staining in beta-TC6 cell focal adhesions, and (iv decreased beta-TC6 cell phosphorylation of ERK(T202/Y204, FAK(Y397 and FAK(Y576. Furthermore, co-culture also resulted in cadherin and beta-catenin accumulations at the NCSC/beta-TC6 cell junctions. Finally, the gap junction inhibitor carbenoxolone did not affect cytokine-induced beta-cell death during co-culture with NCSCs. CONCLUSION: In summary, direct contacts, but not soluble factors, promote improved beta-TC6 viability when co-cultured with NCSCs. We hypothesize that cadherin junctions between NCSC and beta-TC6 cells promote powerful signals that maintain beta

  15. Testing for a cultural influence on reading for meaning in the developing brain: the neural basis of semantic processing in Chinese children

    Tai-Li Chou


    Full Text Available Functional magnetic resonance imaging (fMRI was used to explore the neural correlates of semantic judgments in a group of 8- to 15-year-old Chinese children. Participants were asked to indicate if pairs of Chinese characters presented visually were related in meaning. The related pairs were arranged in a continuous variable according to association strength. Pairs of characters with weaker semantic association elicited greater activation in the mid ventral region (BA 45 of left inferior frontal gyrus, suggesting increased demands on the process of selecting appropriate semantic features. By contrast, characters with stronger semantic association elicited greater activation in left inferior parietal lobule (BA 39, suggesting stronger integration of highly related features. In addition, there was a developmental increase, similar to previously reported findings in English, in left posterior middle temporal gyrus (BA 21, suggesting that older children have more elaborated semantic representations. There were additional age-related increases in the posterior region of left inferior parietal lobule and in the ventral regions of left inferior frontal gyrus, suggesting that reading acquisition relies more on the mapping from orthography to semantics in Chinese children as compared to previously reported findings in English.

  16. 论文化心理认知功能在话语意义建构中的作用%On Functions of Cultural Psychological Cognition in the Meaning Construction of Discourses



    在话语意义建构过程中,文化心理的认知功能为话语语义传递提供了认知心理基础的舞台,这不仅能为表述者展现表述的心理场景,而且还为表述者提供了因心理预期而产生的心理空间,从而使话语操控成为可能,以图控制他人的思维和行为。%Cultural psychological cognitive function in the meaning construction of discourses not only plays the role of psychological cognitive base to transfer the meaning of discourses with psychological scenes for users,but also the role of psychological expectations on the level of thinking for discourse manipulators to control other peoples'thingking and actions.

  17. 产淀粉酶菌种的分离纯化及培养条件研究%S tudy on Separ ation, Purification and Culture Conditions of an Amylase Producing Strain

    纪韦韦; 张小华; 高静


    [目的]探究产淀粉酶菌种的分离纯化及培养条件。[方法]从土壤中分离到1株产淀粉酶能力较强的细菌A并分离纯化培养。[结果]细菌A在以淀粉为碳源的培养基上产淀粉酶能力最强,水解圈平均直径/菌种平均直径(R2/R1)为2.69;在以硝酸钠为氮源的培养基上产淀粉酶能力最强,R2/R1为2.69;pH在7.5时产淀粉酶能力最强,R2/R1为2.95。[结论]以淀粉为碳源、以硝酸钠为氮源、 pH在7.5的培养基有利于细菌A产淀粉酶能力的提高。%Objective] To explore the separation, purification and culture conditions of an amylase producing strain.[Method] Bacterium A, which had a strong ability to produce amylase, was isolated from the soil and then purified and cultured.[ Result] Bacterium A had the stron-gest ability to produce amylase in the medium using starch as the carbon source, and the ratio of the average diameter of hydrolyzation circles R2 to the average diameter of the strain R1(called R2/R1 for short) was 2.69;when being cultured in the medium using sodium nitrate as the nitrogen source, bacterium A had the strongest ability to produce amylase, and R2/R1 was 2.69;as the pH of the medium was 7.5, the ability to produce amylase was the strongest, and R2/R1 was 2.95.[Conclusion] Bacterium A should be cultured in the medium where starch and so-dium nitrate are as the carbon source and the nitrogen source and pH is 7.5 to improve the ability to produce amylase.

  18. Cultural diversity

    Raghavan, Raghu


    The concept of cultural diversity has emerged as an influential one having impact on multiple policy and legal instruments especially following the adoption of the UNESCO Convention on the Protection and Promotion of the Diversity of Cultural Expressions in 2005. The discussions on its appropriate implementation are however profoundly fragmented and often laden with political considerations. The present brief paper offers some thoughts on the meaning of cultural diversity and its implementati...

  19. Distribution of Off-Flavor Compounds and Isolation of Geosmin-Producing Bacteria in a Series of Water Recirculating Systems for Rainbow Trout Culture

    Pre-harvest “off-flavor” in aquaculture products results in large economic losses to producers due to delayed harvest. The common off-flavors “earthy” and “musty” are due to the presence of geosmin and 2-methylisoborneol (MIB), respectively. Although certain species of cyanobacteria are responsibl...

  20. 5-Alkyl-1,2,3,4-tetrahydroquinolines, new membrane-interacting lipophilic metabolites produced by combined culture of Streptomyces nigrescens and Tsukamurella pulmonis.

    Sugiyama, Ryosuke; Nishimura, Shinichi; Ozaki, Taro; Asamizu, Shumpei; Onaka, Hiroyasu; Kakeya, Hideaki


    Eight novel 5-alkyl-1,2,3,4-tetrahydroquinolines (5aTHQs) bearing different side chains have been isolated from a combined culture of Streptomyces nigrescens HEK616 and Tsukamurella pulmonis TP-B0596. The chemical structures including the absolute configuration were elucidated by spectroscopic analysis and total synthesis. 5aTHQs inhibited the growth of wild-type fission yeast while only weakly inhibiting the growth of several mutant strains synthesizing premature ergosterol. These results demonstrate that 5aTHQs are novel antifungals that may target cell membranes.

  1. Linguistic capital and development capital in a network of cultural producers: Mutually valuing peer groups in the ‘interactive fiction’ retrogaming scene

    Allington, D.


    This article reports on a mixed-methods study of the cultural valuing of ‘interactive fiction’ or ‘text adventure games’: a formerly commercial videogame genre sometimes associated with electronic literature but here argued to be best understood in context of the under-researched phenomenon of ‘retrogaming’ or ‘old school gaming’. It is argued that a model for the study of retrogaming scenes is provided in Lena and Peterson’s account of ‘traditionalist’ musical genres, and that these in turn ...

  2. Interleukin-1beta exacerbates hypoxia-induced neuronal damage, but attenuates toxicity produced by simulated ischaemia and excitotoxicity in rat organotypic hippocampal slice cultures.

    Pringle, A K; Niyadurupola, N; Johns, P; Anthony, D C; Iannotti, F


    Using organotypic hippocampal slice cultures we have investigated the actions of Interleukin-1 (IL-1) in a number of injury paradigms. Low concentrations of IL-1 potentiated hypoxia-induced neurodegeneration whilst high concentrations had no effect. In contrast, higher concentrations of IL-1 were strongly neuroprotective in models of combined oxygen/glucose deprivation and N-methyl-D-aspartate toxicity, but no potentiation was observed at low IL-1 concentrations. Both protective and toxic effects of IL-1 were fully antagonized by IL-1 receptor antagonist. These data demonstrate that the effects of IL-1 on neuronal injury are complex, and may be directly related to the injury paradigm studied.

  3. Meaning construction in remembering

    Wagoner, Brady


    F.C. Bartlett and L.S.Vygotsky were two seminal figures in the psychological study of remembering. Both emphasized the role of meaning and imagination in this process. Bartlett did this by showing the systematic and holistic changes that ensue when cultural material is repeatedly reproduced outside...... of the group to which it belongs. Vygotsky, on the other hand, attended to the ways in which we construct meaningful ―signs‖ as artificial memory aids to solve memory problems that go beyond our natural capacities. These two approaches are brought together to show how we utilize various cultural resources...

  4. Religion as memory: How has the continuity of tradition produced ...


    Jun 5, 2015 ... theory, in the postmodern world the continuity of religious memory has been broken and all ... at showing, firstly, in what sense religion can be conceived of as memory ... tradition has produced collective meanings for a number of cultures ... memory in both pre- and postmodern societies, as captured and ...

  5. Genotyping of starter cultures of Bacillus subtilis and Bacillus pumilus for fermentation of African locust bean (Parkia biglobosa) to produce Soumbala.

    Ouoba, Labia Irène Ivette; Diawara, Bréhima; Amoa-Awua, Wisdom kofi; Traoré, Alfred Sababénedyo; Møller, Peter Lange


    Bacillus spp. are the predominant microorganisms in fermented African locust bean called Soumbala in Burkina Faso. Ten strains selected as potential starter cultures were characterised by PCR amplification of the16S-23S rDNA intergenic transcribed spacer (ITS-PCR), restriction fragment length polymorphism of the ITS-PCR (ITS-PCR RFLP), pulsed field gel electrophoresis (PFGE) and sequencing of the 968-1401 region of the 16S rDNA. In previous studies, the isolates were identified by phenotyping as Bacillus subtilis and Bacillus pumilus. The phenotyping was repeated as a reference in the present study. The ITS-PCR and ITS-PCR RLFP allowed a typing at species level. The PFGE was more discriminative and allowed a typing at strain level. Full agreement with the phenotyping was observed in all cases. The sequencing of the 16S rDNA allowed the identification at species level with an identity from 97% to 100% comparing the sequences to those from the GenBank databases. The desired cultures of B. subtilis and B. pumilus from African locust bean fermentation were distinguished by ITS-PCR and ITS-PCR RLFP from Bacillus cereus and Bacillus sphaericus which sometimes occur in the beginning of the fermentation.

  6. Comparative analysis of bioactive N-alkylamides produced by tissue culture raised versus field plantlets of Spilanthes ciliata using LC-Q-TOF (HRMS).

    Bhat, Zubair Shanib; Jaladi, Neeharika; Khajuria, Ravi Kant; Shah, Zeeshan Hamid; Arumugam, Neelakantan


    Spilanthes ciliata (S. ciliata) is a perennial herb of global importance owing to its luscious source of bioactive fatty acid derived amides known as N-alkylamides. It finds application in skin creams, mouth gels and toothpastes. Despite multifaceted applications, a major limitation associated for its commercial application is the scarcity of contamination free plant source, fluctuations in active metabolites due to variation in extraction procedures, and lack of rapid qualitative method for alkylamide profiling. In the current work, attempts were made to 1) optimize conditions for mass propagation of contamination free plants of S. ciliata by tissue culture using leaf discs as explants, 2) establish an optimum extraction ratio of plant/solvent (w/v) for maximum elution of alkylamides and 3) develop a rapid method for qualitative estimation of alkylamide from in vitro raised plants in comparison with that of the field grown counterpart by using LC-Q-TOF (HRMS). To the best of our knowledge, this is the first qualitative report on alkylamide profile of micropropagated whole plant of Spilanthes. The correlation pattern reported in this study may form the basis for using tissue culture raised plantlets of S. ciliata as potential source of bioactive alkylamides on industrial scale.

  7. Co-stimulation of cultured peripheral blood mononuclear cells from intrinsic asthmatics with exogenous recombinant IL-6 produce high levels of IL-4-dependent IgE.

    Sánchez-Guerrero, I M; Herrero, N; Muro, M; Vegara, R P; Campos, M; García-Alonso, A M; Alvarez, M R


    Asthma is an inflammatory airway disorder, traditionally subdivided into extrinsic, immunoglobulin E (IgE)-mediated, and intrinsic asthma of unknown aetiology. IgE synthesis requires contact between T- and B-cells and a signal provided by interleukin (IL)-4, which can be modulated by IL-6. The objective of this study was to evaluate the effects of IL-4 and IL-6 on total IgE synthesis by peripheral blood mononuclear cells from intrinsic and extrinsic asthmatics. Peripheral blood mononuclear cells from intrinsic and extrinsic asthmatic patients and from healthy subjects were cultured and stimulated with pokeweed mitogen, recombinant IL-4 and IL-6. The IgE level in serum and supernatants was measured by an enzyme-linked immunoassay. Serum IgE was significantly lower in intrinsic asthma than in extrinsic asthma, but significantly higher than in control subjects. IgE production by cultured mononuclear cells from extrinsic asthmatics was not modified after exogenous IL-4 and IL-6 addition. However, intrinsic asthmatics showed enhancement of IgE synthesis in response to IL-4 stimulation, reaching a threefold increase of the spontaneous IgE values, when simultaneous recombinant IL-4 plus IL-6 stimulus was used. Our results indicate that exogenous recombinant interleukin-6 can significantly upregulate the interleukin-4-dependent immunoglobulin E synthesis in intrinsic asthma. This suggests that immunoglobulin E could also play a role in the pathogenesis of intrinsic asthma, in which an interleukin-6 threshold would be critical.

  8. The Cultural Meaning of Hundred Kids Playing Games by Jiao Bingzhen of Qing Dynasty%清代焦秉贞《百子团圆图》的文化内涵



    “图像”已经成为当代人文社科研究的一个热点话题,尤其是美术史中的图像,它不仅是人类审美情感的一种符号,更是蕴含了诸如哲学、民俗学、伦理学和教育学等文化内涵的载体。如清代焦秉贞的《百子团圆图》,正是这类图像的典型。该图以生动、鲜活的艺术形象,叙述了游戏在古代儿童教育中的重要作用,进而彰显了其所传递的文化信息。%The word “image”has become a popular topic in contemporary humanity and social science research. Images in the history of fine arts,in particular,are not only a kind of symbols of human aesthetic emotions but a kind of culture vehicles containing philosophical, folkloristic, ethical and educational meanings. Hundred Kids Playing Games by Jiao Bingzhen,a traditional Chinese painting master of the Qing dynasty is an epitome of such images. The painting illustrates the importance of games to early childhood education of ancient China using vivid and lively images and thus highlights the cultural meaning it conveys.

  9. Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA to neuronal cells

    Krynska Barbara


    Full Text Available Abstract Background Although extensive research has been performed to control differentiation of neural stem cells – still, the response of those cells to diverse cell culture conditions often appears to be random and difficult to predict. To this end, we strived to obtain stabilized protocol of NHA cells differentiation – allowing for an increase in percentage yield of neuronal cells. Results Uncommitted GFAP and SOX2 positive neural progenitors – so-called, Normal Human Astrocytes (NHA were differentiated in different environmental conditions to: only neural cells consisted of neuronal [MAP2+, GFAP-] and glial [GFAP+, MAP2-] population, non-neural cells [CD44+, VIMENTIN+, FIBRONECTIN+, MAP2-, GFAP-, S100β-, SOX2-], or mixture of neural and non-neural cells. In spite of successfully increasing the percentage yield of glial and neuronal vs. non-neural cells by means of environmental changes, we were not able to increase significantly the percentage of neuronal (GABA-ergic and catecholaminergic over glial cells under several different cell culture testing conditions. Supplementing serum-free medium with several growth factors (SHH, bFGF, GDNF did not radically change the ratio between neuronal and glial cells – i.e., 1,1:1 in medium without growth factors and 1,4:1 in medium with GDNF, respectively. Conclusion We suggest that biotechnologists attempting to enrich in vitro neural cell cultures in one type of cells – such as that required for transplantology purposes, should consider the strong limiting influence of intrinsic factors upon extracellular factors commonly tested in cell culture conditions.

  10. Cultural ergonomics in interactional and experiential design: conceptual framework and case study of the Taiwanese twin cup.

    Lin, Chih-Long; Chen, Si-Jing; Hsiao, Wen-Hsin; Lin, Rungtai


    Cultural ergonomics is an approach that considers interaction- and experience-based variations among cultures. Designers need to develop a better understanding of cultural ergonomics not just to participate in cultural contexts but also to develop interactive experiences for users. Cultural ergonomics extends our understanding of cultural meaning and our ability to utilize such understanding for design and evaluate everyday products. This study aims to combine cultural ergonomics and interactive design to explore human-culture interaction in user experiences. The linnak is a typical Taiwanese aboriginal cultural object. This study examined the cultural meaning and operational interface of the linnak, as well as the scenarios in which it is used in interaction and user experiences. The results produced a cultural ergonomics interface for examining the manner in which designers communicate across cultures as well as the interweaving of design and culture in the design process.

  11. Significados e sentidos da música: uma breve "composição" a partir da psicologia histórico-cultural Significados y sentidos de la música: una breve "composición" a partir de la psicología histórico-cultural Meanings and senses of music: a brief composition from cultural-historical psychology

    Patrícia Wazlawick


    Full Text Available Este artigo discute a construção dos significados e sentidos da música a partir da Psicologia Histórico-Cultural, principalmente com a contribuição de L. S. Vygotski, buscando tecer uma interface com as idéias dos musicoterapeutas noruegueses Even Ruud e Brynjulf Stige, com base na filosofia de Ludwig Wittgenstein. Quando se vivencia a música, não se estabelece relação apenas com a matéria musical em si, mas com toda uma rede de significados construídos no mundo social, em contextos coletivos mais amplos e em contextos singulares. Dessa forma, os significados e sentidos da música são construídos a partir do contexto social, econômico, político, de vivências concretas e da "utilização viva" da música por sujeitos em relação, onde articulam sua dimensão afetiva, desejos e motivações.Este artículo discute la construcción de los significados y sentidos de la música a partir de la Psicología Histórico Cultural, principalmente con la contribución de L. S. Vygotski, buscando formar una conexión con las ideas de los musicoterapeutas noruegos Even Ruud y Brynjulf Stige, en base a la filosofía de Ludwig Wittgenstein. Cuando se vivencia la música, no sólo se relaciona con la materia musical en sí, sino con toda una red de significados construidos en el mundo social, en contextos colectivos más amplios y en contextos singulares. De esa forma, los significados y sentidos de la música son construidos a partir del contexto social, económico, político, de vivencias concretas y de la "utilización viva" de la música por sujetos interrelacionados, donde articulan su dimensión afectiva, deseos y motivaciones.The construction of meanings and senses in music from the point of view of Cultural-Historical Psychology, with special reference to L. S. VygotskI, is discussed. An interface with the ideas of Norwegian music-therapists Even Ruud and Brynjulf Stige, grounded on Ludwig Wittgenstein's philosophy, is highlighted. The

  12. Optimization of culture conditions to produce high yields of active Acetobacter sp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones

    Chen Xiao-Hong


    Full Text Available Abstract Background Chiral alcohols are widely used in the synthesis of chiral pharmaceuticals, flavors and functional materials and appropriate whole-cell biocatalysts offer a highly enantioselective, minimally polluting route to these valuable compounds. The recently isolated strain Acetobacter sp. CCTCC M209061 showed exclusive anti-Prelog stereoselectivity for the reduction of prochiral ketones, but the low biomass has limited its commercialization and industrial applications. To tackle this problem, the effects of medium components and culture conditions on the strain's growth and reduction activity were explored. Results By using a one-at-a-time method and a central composite rotatable design (CCRD, the optimal medium and culture conditions were found to be as follows: glucose 8.26 g/L, fructose 2.50 g/L, soy peptone 83.92 g/L, MnSO4·H2O 0.088 g/L, pH 5.70, 30°C and 10% (v/v inoculum. Under the above-mentioned conditions, the biomass after 30 h cultivation reached 1.10 ± 0.03 g/L, which was 9.5-fold higher than that obtained with basic medium. Also, the reduction activity towards 4'-chloroacetophenone was markedly enhanced to 39.49 ± 0.96 μmol/min/g from 29.34 ± 0.65 μmol/min/g, with the product e.e. being above 99%. Comparable improvements were also seen with the enantioselective bioreduction of 4-(trimethylsilyl-3-butyn-2-one to the key pharmaceutical precursor (R - 4-(trimethylsilyl-3-butyn-2-ol. Conclusions The biomass and reduction activity of Acetobacter sp. CCTCC M209061 can be greatly enhanced through the optimization strategy. This facilitates use of the strain in the anti-Prelog stereoselective reduction of prochiral ketones to enantiopure chiral alcohols as building blocks for many industries.

  13. Enzymic synthesis of o-succinylbenzoyl-CoA in cell-free extracts of anthraquinone producing Galium mollugo L. cell suspension cultures.

    Heide, L; Kolkmann, R; Arendt, S; Leistner, E


    o-Succinylbenzoic acid (OSB) is an intermediate in the biosynthesis of shikimatederived anthraquinones. The cell free activation of o-succinylbenzoic acid in extracts of anthraquinone producing cells of Galium mollugo L. is demonstrated for the first time. This activation depends on the presence of ATP, coenzyme A and Mg(2+). The o-succinylbenzoic acid coenzyme A ester was identified by converting it to 1,4-dihydroxy-2-naphthoic acid by a bacterial enzyme, viz. naphthoatesynthase. It is thus demonstrated that the o-succinylbenzoic acid coenzyme A ester derived from bacteria and from Galium mollugo cells are identical.

  14. Significados culturales del concepto de salud en el trabajo en profesionales en formación Cultural meanings of the concept of health at work in professionals in training

    María de los Ángeles Aguilera Velasco


    Full Text Available Introducción: El concepto de salud en el trabajo implica una serie de significados para los profesionales de la salud ocupacional, quienes los utilizan para orientar, interactuar, discutir, definir, categorizar e interpretar sus prácticas. Conocer dichos significados es relevan te porque permite aportar datos útiles para el diseño de programas educativos tendientes a la prevención de accidentes y enfermedades laborales. Objetivo: Explorar los significados culturales del concepto de salud en el trabajo, antes y después de un diplomado con duración de seis meses, en profesionales de la salud ocupacional. Metodología: Estudio cualitativo con diseño descriptivo exploratorio. Realizado en Guadalajara, México de febrero a julio de 2011. Los datos fueron recolectados con la técnica de listados libres y analizados a través del modelo de dominios culturales. Resultados: El concepto de salud en el trabajo, antes del diplomado se definió a través de los términos riesgo y seguridad. Después del diplomado, la salud en el trabajo se definió a través de los elementos seguridad y prevención. Conclusiones: La cultura de la prevención se logra a través de un proceso de aprendizaje que incluye conocimientos sobre los riesgos laborales y la normatividad vigente.Introduction: The concept of health in the workplace involves a number of meanings for occupational health professionals, who are used to guide, interact, discuss, define, categorize, and interpret their practices. Knowing these meanings is relevate because it allows to provide useful data for the design of educational programmes aimed at the prevention of accidents and occupational diseases. Objective: Explore the cultural meanings of the concept of health at work, before and after the training with duration of six months, in occupational health professionals. Methodology: Qualitative study with exploratory descriptive design, in Guadalajara, Mexico from February to July 2011. The data

  15. Exploring the Meaning of "Tiger Mother" from the Perspectives of Children in the Different Cultures%探索不同文化背景下儿童眼中“虎妈”的意义



    儿童的自然成长具有普遍一致的规律,而我们在寻求发展客观规律的同时也不得不考虑文化教育的影响。本文从发展心理学的研究角度,探讨儿童在父母教养和社会文化环境中社会心理调整和学业努力的特征,以期建立适当的文化环境来促进青少年的发展。研究中,我们调查了中关两国儿童对其父母权威和父母教养实践的感知,并分析了儿童解释父母采取如此的实践的原因。基于父母教养方式的跨文化研究,理解在不同文化背景下儿童的社会心理与生活体验相互作用的意义,以进一步探索儿童在社会文化环境中的自主与调节。%There are some common foundations for the children' s natural development, while the social and cultural contexts for children development should be considered as an important factor in exploring these foundations. Expecting for constructing a beneficial cultural environment for children development, the characteristics of children' s psychological adjustments and school efforts influenced by parenting practices in social and cultural contexts is discussed in the paper. In the study, we investi- gated American and Chinese children' s perceptions on the parental authorities and practices, and analyzed children' s interpretations on the reasons of such parental practices. The purpose of the study is to understanding the meanings of the interaction between children' s social psychological adjustment and their social experience in different cultural contexts, in order to explore children' s autonomy and psychological adjustment in social and cultural environments.

  16. Impact of active soil carbonate and burn size on the capacity of the rockrose Cistus laurifolius to produce Tuber melanosporum carpophores in truffle culture.

    García-Montero, Luis G; Casermeiro, Miguel A; Manjón, José L; Hernando, Isabel


    There are very few studies on Tuber melanosporum associated with Cistus populations. In central Spain, we confirm that C. laurifolius shows carpophore production of Tuber melanosporum. This study demonstrates a correlation between T. melanosporum production and the size of the 41 burns, with burn size explaining 26% of the variability in carpophore production. However, statistical analysis of the results indicates that average production of 21 burns with Cistus laurifolius is 73% lower than the production of 20 burns associated with Quercus/Corylus without Cistus laurifolius in this zone, respectively. C. laurifolius develops small burns, which has an impact on their carpophore production, and their soils have 92% less active carbonate than the burns associated with Quercus/Corylus in this zone, respectively. The low levels of active carbonate allow Cistus laurifolius to grow, but impair Tuber melanosporum production. We thus provide a mechanism, based on the inability of Cistus laurifolius to grow in highly carbonated soils, which contradicts the well-known fact that C. laurifolius might act as a transmitter of Tuber melanosporum mycorrhizae. These results indicate that this rockrose can not be considered a useful species for truffle culture. We thus recommend that particular attention should be given to the concentration of active carbonate present in the soil in future studies on the relationship between Cistus species and truffles.


    Anupama Gupta


    Full Text Available Purified bacteriocin produced from Brevibacillus borstelensis AG1 isolated from Marcha a local wine starter herbal cake, was used to enhance the shelf life of tomato paste. Preservative effect of purified bacteriocin was studied for nine days in tomato paste inoculated with food borne pathogens and was compared to commercial biopreservative – nisin and chemical preservative – sodium benzoate. The indicator strains i.e. Listeria monocytogenes MTCC839, Bacillus subtilis CRI and Clostridium perfringens MTCC1739 were used at the amount 8.16, 8.13 and 8.18 log CFU/ml. Viable cells were counted periodically and a consistent reduction in number of viable cells of each tested pathogen was observed. It was found antagonistic against L. monocytogenes MTCC839, B. subtilis CRI and C. perfringes MTCC1739 which are the most challengeable and food borne pathogens found in processed vegetables products. Purified bacteriocin was found active over a wide pH range i.e. 3.0 to 11.0 and was able to withstand temperature up to 100oC. It showed a better preservative potential by reducing pathogenic load of the tested strains (by 2.02, 2.05 and 2.02 log cycles (CFU/ml of L. monocytogenes MTCC839, B. subtilis CRI and C. perfringes MTCC1739, respectively in tomato paste as compared to control (without bacteriocin. This proves efficiency of bacteriocin produced by B. borstelensis AG1 as biopreservative to enhance the safety and shelf life of acidic foods.

  18. Evaluation of economically feasible, natural plant extract-based microbiological media for producing biomass of the dry rot biocontrol strain Pseudomonas fluorescens P22Y05 in liquid culture.

    Khalil, Sadia; Ali, Tasneem Adam; Skory, Chris; Slininger, Patricia J; Schisler, David A


    The production of microbial biomass in liquid media often represents an indispensable step in the research and development of bacterial and fungal strains. Costs of commercially prepared nutrient media or purified media components, however, can represent a significant hurdle to conducting research in locations where obtaining these products is difficult. A less expensive option for providing components essential to microbial growth in liquid culture is the use of extracts of fresh or dried plant products obtained by using hot water extraction techniques. A total of 13 plant extract-based media were prepared from a variety of plant fruits, pods or seeds of plant species including Allium cepa (red onion bulb), Phaseolus vulgaris (green bean pods), and Lens culinaris (lentil seeds). In shake flask tests, cell production by potato dry rot antagonist Pseudomonas fluorescens P22Y05 in plant extract-based media was generally statistically indistinguishable from that in commercially produced tryptic soy broth and nutrient broth as measured by optical density and colony forming units/ml produced (P ≤ 0.05, Fisher's protected LSD). The efficacy of biomass produced in the best plant extract-based media or commercial media was equivalent in reducing Fusarium dry rot by 50-96% compared to controls. In studies using a high-throughput microbioreactor, logarithmic growth of P22Y05 in plant extract-based media initiated in 3-5 h in most cases but specific growth rate and the time of maximum OD varied as did the maximum pH obtained in media. Nutrient analysis of selected media before and after cell growth indicated that nitrogen in the form of NH4 accumulated in culture supernatants, possibly due to unbalanced growth conditions brought on by a scarcity of simple sugars in the media tested. The potential of plant extract-based media to economically produce biomass of microbes active in reducing plant disease is considerable and deserves further research.

  19. Economic process to produce biohydrogen and volatile fatty acids by a mixed culture using vinasse from sugarcane ethanol industry as nutrient source.

    Sydney, Eduardo Bittencourt; Larroche, Christian; Novak, Alessandra Cristine; Nouaille, Regis; Sarma, Saurabh Jyoti; Brar, Satinder Kaur; Letti, Luiz Alberto; Soccol, Vanete Thomaz; Soccol, Carlos Ricardo


    This work evaluates the potential of vinasse (a waste obtained at the bottom of sugarcane ethanol distillation columns) as nutrient source for biohydrogen and volatile fatty acids production by means of anaerobic consortia. Two different media were proposed, using sugarcane juice or molasses as carbon source. The consortium LPBAH1 was selected for fermentation of vinasse supplemented with sugarcane juice, resulting in a higher H2 yield of 7.14 molH2 molsucrose(-1) and hydrogen content in biogas of approx. 31%, while consortium LPBAH2 resulted in 3.66 molH2/molsucrose and 32.7% hydrogen content in biogas. The proposed process showed a rational and economical use for vinasse, a mandatory byproduct of the renewable Brazilian energy matrix.

  20. Retaining the meaning of the words religiousness and spirituality: a commentary on the WHOQOL SRPB group's "a cross-cultural study of spirituality, religion, and personal beliefs as components of quality of life" (62: 6, 2005, 1486-1497).

    Moreira-Almeida, Alexander; Koenig, Harold G


    Recent years have seen increasing recognition paid to the relation of religiousness/spirituality (R/S) to health care and research. This has led to the development of more inclusive and trans-culturally validated measurements of R/S. This paper comments on the WHOQOL SRPB Group's "A cross-cultural study of spirituality, religion, and personal beliefs as components of quality of life" (62: 6, 2005, 1486-1497), a recently published paper in Social Science & Medicine, and illustrates a possible problem in the measurement of R/S, especially as related to the study of mental health outcomes. Some scales have included questions about psychological well-being, satisfaction, connectedness with others, hopefulness, meaning and purpose in life, or altruistic values as part of their measure of R/S. These questions are really tapping indicators of mental health, and should not be included in the definition of R/S itself. Otherwise, tautology is the result, and it should not be surprising that such measures of R/S (defined by questions tapping mental health) are related to mental health outcomes.

  1. Stimulation of butyrate production by gluconic acid in batch culture of pig cecal digesta and identification of butyrate-producing bacteria.

    Tsukahara, Takamitsu; Koyama, Hironari; Okada, Masaaki; Ushida, Kazunari


    Gluconic acid reaches the large intestine to stimulate lactic acid bacteria. However, the fermentation pattern of gluconic acid has yet to be elucidated. Accordingly, we examined the fermentation properties induced by gluconic acid in the pig cecal digesta in vitro. We also tested sorbitol and glucose, substrates for which the fermentation rate and patterns are known. The gluconic acid-utilizing bacteria were further isolated from pig cecal digesta and identified to examine the effect of gluconic acid on hind gut fermentation. Gluconic acid was fermented more slowly than were the other two substrates. Gluconic acid stimulated butyrate production; the butyrate molar percentage reached 26%, which is considered a high butyrate production. The majority of gluconic acid fermenters were identified as lactic acid bacteria, such as Lactobacillus reuteri and L. mucosae, and acid-utilizing bacteria, such as Megasphaera elsdenii and Mitsuokella multiacida. The gluconic acid fermented by lactic acid bacteria, and the lactate and acetate that were produced were used to form butyrate by acid-utilizing bacteria, such as M. elsdenii. Gluconic acid may be useful as a prebiotic to stimulate butyrate production in the large intestine.

  2. Human mesenchymal stem/stromal cells (hMSCs) cultured as spheroids are self-activated to produce prostaglandin E2 (PGE2) that directs stimulated macrophages into an anti-inflammatory phenotype

    Ylöstalo, Joni H.; Bartosh, Thomas J.; Coble, Katie; Prockop, Darwin J.


    Culturing cells in 3D provides an insight into their characteristics in vivo. We previously reported that human mesenchymal stem/stromal cells (hMSCs) cultured as 3D spheroids acquire enhanced anti-inflammatory properties. Here we explored the effects of hMSC spheroids on macrophages that are critical cells in the regulation of inflammation. Conditioned medium from hMSC spheroids inhibited LPS-stimulated macrophages from secreting pro-inflammatory cytokines TNFα, CXCL2, IL6, IL12p40, and IL23. Conditioned medium also increased the secretion of anti-inflammatory cytokines IL10 and IL1ra by the stimulated macrophages, and augmented expression of CD206, a marker of alternatively activated M2 macrophages. The principal anti-inflammatory activity in conditioned medium had a small molecular weight, and microarray data suggested that it was PGE2. This was confirmed by the observations that PGE2 levels were markedly elevated in hMSC spheroid-conditioned medium, and that the anti-inflammatory activity was abolished by an inhibitor of COX-2, a silencing RNA for COX-2, and an antibody to PGE2. The anti-inflammatory effects of the PGE2 on stimulated macrophages were mediated by the EP4 receptor. Spheroids formed by human adult dermal fibroblasts produced low levels of PGE2 and displayed negligible anti-inflammatory effects on stimulated macrophages, suggesting the features as unique to hMSCs. Moreover, production of PGE2 by hMSC spheroids was dependent on the activity of caspases and NFκB activation in the hMSCs. The results indicated that hMSCs in 3D-spheroid cultures are self-activated, in part by intracellular stress responses, to produce PGE2 that can change stimulated macrophages from a primarily pro-inflammatory M1 phenotype to a more anti-inflammatory M2 phenotype. PMID:22865689

  3. Cultural Meaning of Scattered Geometric Motif on Silk Textiles during Qin and Han Dynasties%秦汉丝织品上散点几何纹的文化含义

    张文; 徐纯中; 邱夷平


    Scattered geometric motif brocade during Qin and Han dynasties unearthed in Hubei and Hunan provinces has attracted the attention of many researchers with its mysterious pattern. On this basis, this paper studies the pattern composition of scattered geometric motif, compares similarities and differences of scattered geometric motif and geometric motif pattern in Shang and Zhou dynasties and ancient hieroglyph and further studies the relationship between scattered geometric motif pattern and ancient witch culture of the Kingdom of Chu and symbolic meanings of scattered geometric motif pattern with iconological method. The research considers that scattered geometric motif is the representative influenced by witch culture and its pattern change and scatter represent the symbolic meaning while having practical applicability. Technical improvement has created scattered geometric motif; meanwhile, scattered geometric motif has gradually developed into a culture and promoted further technological development.%两湖地区出土的秦汉时期的散点几何纹锦以其神秘的纹样吸引了很多研究者的注意,基于此,研究了散点几何纹的图案构成,比较了散点几何纹图案与商周时期几何纹图案和古象形文字的异同,并运用图像学方法进一步研究了散点几何纹图案与楚国古老的巫觋文化的关系,以及散点几何纹图案所包含的象征意义.研究认为,散点几何纹是受到巫觋文化影响的代表,它的图案的变化和打散具有实用性的同时也代表了象征的意义.技术的革新造就了散点几何纹,同时散点几何纹也逐渐融合演变成一种文化,促进了技术的进一步发展.

  4. Disney animation: Global diffusion and local appropriation of culture

    Souad Belkhyr


    Children’s media products reflect the cultural values of their producers and the social, political and economic conditions under which they were produced. Watching an animated feature, therefore, cannot be regarded as an innocent and simple act of consumption. It rather involves a complex process of coding/decoding and appropriating cultural meanings. The relation between structured patterns of communication under globalization, on one hand, and the local conditions under which Disney cartoon...

  5. 一株产过氧化氢酶细菌的筛选及发酵条件的优化%Screening of catalase-producing strain and optimization of culture conditions

    陈欣; 甘国斌; 孙敬冉; 陈黎黎; 唐少宇


    Catalase is widely applied in the food, textile and chemical industries. A strain, which was a catalase over-producer, was isolated and identified as Bacillus sp. Single-factor experiments including glucose concentration , NaNO3 concentration, culture temperature, culture time, rotary speed, the initial pH of medium were carried out to optimize the conditions for catalase production. Based on this, response surface analysis method was used for further enhancing catalase production. The results showed that the linear and quadratic term of NaNO3 concentration and rotary speed, the quadratic term of culture time, and the interactive effects of NaNO3 concentration with both rotary speed and culture time were significant. The optimal culture conditions were as follows. The NaNO3 concentration was 4. 0 g/L, the cultural time was 30 h, and the rotary speed was 199 r/min. Under these conditions, the maximum enzyme activity reached 211. 84 U/mL.%文中筛选到一株过氧化氢酶生产菌株,初步鉴定为枯草芽孢杆菌(Bacillus sp.)对葡萄糖浓度、NaNO3浓度、培养温度、培养时间、摇床转速和培养基初始pH等对该菌株发酵特性的影响进行了单因素实验,并在此基础上采用响应曲面法(RSA)对其发酵条件进行了进一步优化.结果表明:NaNO3浓度和摇床转速的一次项和二次项、培养时间的二次项、NaNO3浓度和摇床转速的交互项以及NaNO3浓度和培养时间交互项对过氧化氢酶酶活均有显著影响;NaNO3浓度为4.0 g/L、培养时间为30 h和摇床转速为199 r/min时,酶活达到最大值211.84 U/mL.

  6. Medical science, culture, and truth.

    Gillett, Grant


    There is a fairly closed circle between culture, language, meaning, and truth such that the world of a given culture is a world understood in terms of the meanings produced in that culture. Medicine is, in fact, a subculture of a powerful type and has its own language and understanding of the range of illnesses that affect human beings. So how does medicine get at the truth of people and their ills in such a way as to escape its own limited constructions? There is a way out of the closed circle implicit in the idea of a praxis and the engagement with reality that is central to it and the further possibility introduced by Jacques Lacan that signification is never comprehensive in relation to the subject's encounter with the real. I will explore both of these so as to develop a conception of truth that is apt for the knowledge that arises in the clinic.

  7. 从仪式到消费:电视传媒对体育意义生产的文化解读%From ritual to consumption:a cultural interpretation of sports meaning production by TV media



      From the perspectives of classic theories such as semeiology and communication science, the author studied sports meaning production by TV media. In terms of the ritual and consumption meanings of entertainment and aesthetics, the carnival rituals of televised sports are quietly replacing people’s personal practice and independ-ent meditation of sports, becoming a lifestyle of contemporaries, reproducing the surplus value of sports consump-tion culture while the planarized health and body images of athletes and their ritualized performances are eliminat-ing the depth norms of time and thoughts.%  在符号学、传播学等经典理论观照下,就电视媒介对体育意义的生产进行了研究。在娱乐与审美的仪式消费意义上,电视化体育的狂欢仪式正在悄然替代人们对体育的亲身实践,成为当代人的一种生活方式,平面化的运动员健康身体形象及其仪式化表演消除着时间与思想的深度范式的同时,再生产了关于体育的消费文化的剩余价值。

  8. Producing Presences

    Mandagará, Pedro


    Resenha de MENDES, Victor K.; ROCHA, João Cezar de Castro (Eds.). Producing Presences: branching out from Gumbrecht’s work. Dartmouth, Massachusetts: University of Massachusetts Dartmouth, 2007. (Adamastor book series, 2)

  9. Pop Culture Pedagogies: Process and Praxis

    Maudlin, Julie Garlen; Sandlin, Jennifer A.


    As Miller (1999) explained, "it is increasingly important for educators to take seriously the processes by which media texts are produced and disseminated, and to understand the ways in which media images and constructions pervade all our lives" (p. 234). Taking popular culture seriously means making a purposeful commitment to bring…

  10. Pop Culture Pedagogies: Process and Praxis

    Maudlin, Julie Garlen; Sandlin, Jennifer A.


    As Miller (1999) explained, "it is increasingly important for educators to take seriously the processes by which media texts are produced and disseminated, and to understand the ways in which media images and constructions pervade all our lives" (p. 234). Taking popular culture seriously means making a purposeful commitment to bring…

  11. Culture perspectives.

    Locsin, Rozzano C


    All cultures have had means and techniques that express their immediate aims. The thing that interests me is that today, painters do not have to go to a subject matter outside of themselves. They work from a different source. They work from within. It seems to me that the modern artist cannot express this age, the airplane, the atom bomb, the radio, in the old form of the Renaissance or of any of the old cultures.

  12. Cultural Identity Through CLIL

    Oprescu Monica


    The CLIL approach is a modern manner of teaching English, which has been adapted in Romanian schools and universities. An interesting aspect of learning a foreign language is the contact with its culture/s and the changes it produces in terms of identity. Therefore, a challenging question to be answered is whether a CLIL approach focusing on culture influences students' cultural identity.

  13. The influence of metal speciation in combustion waste on the efficiency of Cu, Pb, Zn, Cd, Ni and Cr bioleaching in a mixed culture of sulfur-oxidizing and biosurfactant-producing bacteria.

    Karwowska, Ewa; Wojtkowska, Małgorzata; Andrzejewska, Dorota


    Metal leachability from ash and combustion slag is related to the physico-chemical properties, including their speciation in the waste. Metals speciation is an important factor that influences the efficiency of metal bioleaching from combustion wastes in a mixed culture of acidophilic and biosurfactant-producing bacteria. It was observed that individual metals tended to occur in different fractions, which reflects their susceptibility to bioleaching. Cr and Ni were readily removed from wastes when present with a high fraction bound to carbonates. Cd and Pb where not effectively bioleached when present in high amounts in a fraction bound to organic matter. The best bioleaching results were obtained for power plant slag, which had a high metal content in the exchangeable, bound to carbonates and bound to Fe and Mg oxides fractions- the metal recovery percentage for Zn, Cu and Ni from this waste exceeded 90%.

  14. Optimization of culture conditions of producing bacterial cellulose utilizing starch wastewater%淀粉废水发酵产细菌纤维素发酵条件的优化

    徐伟; 张妍; 傅徐阳


    The culture conditions of Gluconacetobacter xylinus producing bacterial cellulose utilizing corn starch wastewater(adding glucose 20g/L,corn steep liquor 40g/L,ethanol 150mL/L) were investigated through singlefactor and orthogonal tests. The suitable culture conditions were as follows.liquid level was 80mL in 250mL triangle bottle,pH4.0,inoculation volume was 9% (VN),culture temperature was 28℃ ,the the yield of bacterial cellulose reached the peak(4.41g/L) at this time. The bacterial cellulose was verified by FTIR,SEM was used to observe the surface pattern of bacterial cellulose membrane.%以玉米淀粉废水添加葡萄糖20g/L,玉米浆40班,乙醇150mL/L为发酵基质,采用单因素和正交实验设计对葡糖醋杆菌(Gluconacetobacter xylinus)发酵产细菌纤维素条件进行优化。结果表明,最佳发酵条件为:装液量80mL/250mL,pH4.0,接种量9%(V/V),温度28℃;在此条件下得到细菌纤维素产量为4.41g/L。采用傅立叶转换红外光谱FTIR验证产物为细菌纤维素,并由SEM扫描电镜观察纤维素膜表面形貌。

  15. 我国传统文化中知识产权产生与发展不利因素分析%Analysis on Disadvantage of the Produce and Development of Intellectual Property System in Chinese Traditional Culture



    According to the view of new system economics,traditional culture is the informal and recessive system;Intel-lectual property is the formal and dominant system. Chinese traditional culture as a kind of informal system,carries many adverse factors affecting and restricting the produce and development of the intellectual property system. It is especially im-portant that we deeply understand these adverse factors and the influence on strengthening the construction of intellectual system today.%按照新制度经济学的观点,传统文化是非正式制度,是一种隐性制度;知识产权是正式制度,是一种显性制度。我国传统文化作为一种非正式制度,其中有许多不利因素影响和制约着知识产权制度的产生与发展,深刻认识这些不利因素和影响,在不断加强知识产权制度建设的今天意义尤为重大。

  16. A new producer of mevastatin

    Chu; Yiwen; Yang; Xi


    Mevastatin (compactin)was isolated form culture broth of a fungal strain SIIA-F3933,The strain SIIA-F3933 was isolated from a soil sample collected in Putuo mountain,Zhejiang Province,P,R.China,It was identified as Penicillium janczewskii Zaleski based on its cultural and morphological characteristics and was regarded as a new producer of mevastatin,。

  17. Means of Transport

    Balle, Søren Hattesen


    The car is probably one of the most central signifiers of modern scientific and technological progress. Not only did its introduction in early 20th century America quite literally move scientific progress into the street, it also provided its increasing number of users with new and technologically...... improved means of fast physical movement from one place to another. Yet, the impact that the technology of automobility came to have was not of a limited materialistic nature. As many literary and cultural critics have noted, car technology extended its influence into the field of imaginative transport...... as a means of physical and spiritual transport is thus consistently embedded in a less idealized context, where its technological machinery is foregrounded. In conclusion, the paper argues that Williams’s thematic treatment of the car in his writings may be read as a modern poet’s meta-poetic gloss on poetry...

  18. 论新感觉派小说性别叙事的文化寓意%On the Cultural Meanings of Gender Narrative in the Novels of New Sensationalist



    This article considers the dialogue and interaction between sexes as a potential context in the novels of New Sensationalist , and discusses the cultural meanings of the gender narrative .The female rhetoric in the novels of New Sensationalist was represented as the isomorphic relationship between city and women , as well as the compensatory relationship between urban and rural .By means of gazing , scopophilia and disenchantment , male narrators projected the sexual visions and power imagine on the female objects within the somatic discourse , and constructed themselves though the tactics of Misogyny , Sexual sadism and Salvation .The writing of sexes stale-mate, as well as the vague national consciousness and ambiguous colonized expression , revealed the imaginative consistency between modern China and progressive West , and then provided a new valid basis to support the traditional patriarchy .%新感觉派小说的女体修辞表现为城市与女性的同构关系、城市与乡土的补偿关系,男性叙事者通过凝视、窥淫和祛魅手段,在躯体话语中对女性客体投射情欲目光和权力想象,并以厌女、性虐或拯救三种策略进行自我建构。新感觉派的两性对峙书写配合其模糊的民族意识和被殖民的暧昧表达,表现出现代中国与进步西方的一致性想象,进而为维护传统男权立场寻得新的文学合法性依据。


    郑雯; 吴兴泉; 辛惠普; 韦永刚; 杜春梅


    By means of primary studies, we suggest that: the optimum temperature andpH value were respectively at 15~20 ℃ and 3~8; the optimum C and N sources areGlucose and Tryptone.The optimum culture medium is the powder of corn.%本文对春油菜菌核病菌(Aclerotia sclerotiorum (Lib.)Derbary)产生菌核的影响因子进行初步研究。结果表明:菌丝产生菌核的最适温度15~20℃;最适pH 3~8;最适碳源为葡萄糖;最适氮源为胰蛋白胨;最适培养基为玉米粉琼脂培养基。

  20. Quantitative and qualitative research across cultures and languages: cultural metrics and their application.

    Wagner, Wolfgang; Hansen, Karolina; Kronberger, Nicole


    Growing globalisation of the world draws attention to cultural differences between people from different countries or from different cultures within the countries. Notwithstanding the diversity of people's worldviews, current cross-cultural research still faces the challenge of how to avoid ethnocentrism; comparing Western-driven phenomena with like variables across countries without checking their conceptual equivalence clearly is highly problematic. In the present article we argue that simple comparison of measurements (in the quantitative domain) or of semantic interpretations (in the qualitative domain) across cultures easily leads to inadequate results. Questionnaire items or text produced in interviews or via open-ended questions have culturally laden meanings and cannot be mapped onto the same semantic metric. We call the culture-specific space and relationship between variables or meanings a 'cultural metric', that is a set of notions that are inter-related and that mutually specify each other's meaning. We illustrate the problems and their possible solutions with examples from quantitative and qualitative research. The suggested methods allow to respect the semantic space of notions in cultures and language groups and the resulting similarities or differences between cultures can be better understood and interpreted.

  1. Significados e sentidos de saúde socializados por artefatos culturais: leituras das imagens de advertência nos maços de cigarro Senses and meanings of health socialized by cultural devices: readings of the warning images on cigarettes packs

    Rogério Dias Renovato


    possibilities of readings that can be present at these devices.We understand the propagated images as cultural pedagogies that behave as texts to be read, building up representations that can be assumed as true effect, and attributes to the citizen the necessity of constant control of its action. Reassuring the biomedicine and the health institutions, an onedimensional vision is propagated disregarding the complexity of this problem. We understand that the cultural pedagogies are part of competition territories, where the directions and meanings can be re-prepared producing hybrid identities that build up its own identity origins over this tangle of power relations.

  2. Lignolytic enzymes produced by Trametes villosa ccb176 under different culture conditions Enzimas ligninóliticas produzidas por Trametes villosa ccb176 em diferentes condições de cultivo

    Renata Yamanaka


    Full Text Available The expression of the enzymatic system produced by basidiomycetous fungi, which is involved in the degradation of xenobiotics, mainly depends on culture conditions, especially of the culture medium composition. Trametes villosa is a strain with a proven biotechnological potential for the degradation of organochlorine compounds and for the decolorization of textile dyes. The influence of glucose concentration, addition of a vegetable oil-surfactant emulsion, nature of the surfactant and the presence of manganese and copper on the growth, pH and production of laccase, total peroxidase and manganese-dependent peroxidase activities were evaluated. In general, acidification of the medium was observed, with the pH reaching a value close to 3.5 within the first days of growth. Laccase was the main activity detected under the different conditions and was produced throughout the culture period of the fungus, irrespective of the growth phase. Supplementation of the medium with vegetable oil emulsified with a surfactant induced manganese-dependent peroxidase activity in T. villosa. Higher specific yields of laccase activity were obtained with the addition of copper.A expressão do sistema enzimático produzido por fungos basidiomicetos envolvido na degradação de xenobióticos é bastante dependente das condições de cultivo, principalmente da composição do meio de cultivo. Trametes villosa CCB176 é uma linhagem com comprovado potencial biotecnológico para degradação de compostos organoclorados e descoloração de corantes têxteis. Foi avaliada a influência da concentração de glicose, adição de emulsão de óleo vegetal e surfactante, natureza do surfactante e os metais manganês e cobre no crescimento, pH e na produção das atividades de lacase, de peroxidases totais e de peroxidase dependente de manganês. No geral, ocorreu acidificação do meio com pH atingindo valor próximo a 3,5, nos primeiros dias de crescimento. Lacase foi a

  3. Crescimento do fungo simbionte de formigas cortadeiras do gênero Acromyrmex em meios de cultura com diferentes extratos Growth of symbiont fungi of cutter ants of the genus Acromyrmex in means of culture with different extracts

    Regina da Silva Borba


    plants have been accomplished to evaluate the effect on the development of the mushroom symbiont. The objective of this work was to evaluate the influence of added culture means of different extracts on the growth of the L. gongylophorus mushroom. Where mushrooms of the species Acromyrmex ambiguus, Acromyrmex crassispinus, Acromyrmex heyeri and Acromyrmex lundi were inoculated in the means Nutritious Ágar and BDA, both added of molasses and extracts of Lolium multiflorum Lam., Typha angustifolia L. and ant (Atta sexdens piriventris. The Pagnocca medium was used as pattern. The plates were maintained in cameras acclimatized to the temperature of 25 + 1°C and 24 hours of darkness, for a period of 49 days. The fungal growth was evaluated with base in the diameter of the colony, which was accomplished with weekly intervals, totalizing seven evaluations. Also the dry weight was evaluated from the mushroom to the 53 days. The results showed that the mushroom cultivated by different species of ants has differentiated growth in culture means with different extracts.

  4. 离体培养藏红花细胞合成的藏红花色素稳定性%Stabilities of saffron pigments produced by in vitro cell culture of Crocus sativus L

    扶文君; 袁丽红


    The effects of pH, temperature, light, oxidant, reductant, food additives and metal ions on the stabilities of saffron pigments produced by Crocus sativus cells were investigated,and their stabilities were also compared with those of pigments from saffron stigma. The results showed that they were all water⁃solubleand bright yellow. They were stable to food additives and H2 O2 . Compared with saffron stigma pigments, saffron pigments produced by cell culture were more stable to strong acid ( pH 1 ) , high temperature(80 ℃,100 ℃)and light(incandescent light,UV),and less stable to VCand Cu2+(0�001 mol/L)�The coloring power of saffron pigments produced by cell culture could be increased by dilute alkaline solutions(0�47 mol/L Na2CO3,0�1 mol/L NaOH),Na2SO3,Fe2+(0�050 mol/L) and Fe3+.%研究pH、温度、光照、氧化剂、还原剂、食品添加剂和金属离子对离体培养的藏红花细胞合成的藏红花色素稳定性的影响,并与藏红花柱头色素进行比较。结果表明:藏红花细胞合成的色素与柱头色素均为水溶性色素,呈亮黄色,对食品添加剂(食盐、柠檬酸、蔗糖、苯甲酸钠)和H2 O2表现出良好的稳定性。与柱头色素相比,藏红花细胞合成的色素对强酸( pH 1)、高温(80、100℃)和光照(日光灯光、紫外光)表现出更好的稳定性,对 VC和 Cu2+(0�001 mol/L)的稳定性略差。稀碱溶液(0�47 mol/L Na2CO3、0�1 mol/L NaOH)、Na2SO3、Fe2+(0�050 mol/L)和Fe3+能使细胞合成的色素色强增强。

  5. The Politics of Culture

    John Storey


    Full Text Available This article provides an overview over the evolution of thinking about "culture" in the work of Raymond Williams. With the introduction of Antonio Gramsci's concept of hegemony culture came to be understood as consisting of not only shared, but contested meanings as well. On the basis of this redefinition by Williams, cultural studies was able to delineate culture as the production, circulation, and consumption of meanings that become embodied and embedded in social practice.

  6. Caracterização de biopolímeros produzidos por Beijerinckia sp. 7070 em diferentes tempos de cultivo Characterization of biopolymers produced by Beijerinckia sp 7070 at different culture times

    Caroline Dellinghausen Borges


    Full Text Available Biopolímeros são polissacarídeos microbianos. O biopolímero produzido por Beijerinckia sp. 7070 possui comportamento pseudoplástico e apresenta alta viscosidade em baixas velocidades de deformação, conferindo ao polímero excelentes características de suspensão. O objetivo desse trabalho foi caracterizar o biopolímero produzido por Beijerinckia sp. 7070 em diferentes tempos de cultivo, quanto à produção total, produção de polímeros de fibra longa e curta, produtividade, viscosidade e composição química. Os polímeros produzidos em meio YM líquido foram recuperados em diferentes tempos de cultivo, secos e pesados para determinação da produção e produtividade. O tipo de fibra produzido durante o cultivo foi avaliado microscopicamente. Viscosidades aparentes de solução aquosa 1% foram determinadas a 6, 12, 30 e 60rpm, a 25º C, em um viscosímetro Brookfield. A composição do biopolímero foi determinada por cromatografia em camada delgada comparativa. As maiores produções totais encontradas foram em 30 e 72h, a maior produtividade em 48h e a maior viscosidade em 72h. Os polímeros de fibra longa apresentaram uma tendência de tornarem-se mais longos com o tempo. A viscosidade do polímero de fibra longa foi maior que a do de fibra curta. Todos os biopolímeros apresentaram os mesmos componentes (glucose, galactose, fucose e ácido glucurônico mas em concentrações diferentes.Biopolymers are microbial polysaccharides. The biopolymer produced by Beijerinckia sp 7070 has pseudoplastic behaviour and shows high viscosity at low deformation rates, giving to polymer excellent suspension characteristics. The objective of this work was to characterize the biopolymer produced at different culture times by Beijerinckia sp 7070 in relation to total production, production of short and long fiber polymers, productivity, viscosity and chemical composition. The polymers produced in liquid YM medium were recovered at different

  7. In vitro cultured fetal fibroblasts have myofibroblast-associated characteristics and produce a fibrotic-like environment upon stimulation with TGF-β1: Is there a thin line between fetal scarless healing and fibrosis?

    Walraven, M; Akershoek, J J; Beelen, R H J; Ulrich, M M W


    Transforming growth factor-β (TGF-β) is a cytokine occurring in three isoforms with an important function in development and wound healing. In wound healing, prolonged TGF-β signaling results in myofibroblast differentiation and fibrosis. In contrast, the developing second-trimester fetal skin contains high levels of all three TGF-β isoforms but still has the intrinsic capacity to heal without scarring. Insight into TGF-β signal transduction during fetal wound healing might lead to methods to control the signaling pathway during adult wound healing. In this study, we imitated wound healing in vitro by stimulating fibroblasts with TGF-β1 and examining myofibroblast differentiation. The aim was to gain insight into TGF-β signaling in human fibroblasts from fetal and adult dermis. First, TGF-β1 stimulation resulted in similar or even more severe upregulation of myofibroblast-associated genes in fetal fibroblasts compared to adult fibroblasts. Second, fetal fibroblasts also had higher protein levels of myofibroblast-marker α-smooth muscle actin (α-SMA). Third, stimulated fetal fibroblasts in collagen matrices had higher protein levels of α-SMA, produced more of the fibrotic protein fibronectin splice-variant extra domain A (FnEDA), and showed enhanced contraction. Finally, fetal fibroblasts also produced significant higher levels of TGF-β1. Altogether, these data show that in vitro cultured fetal fibroblasts have myofibroblast-associated characteristics and do produce a fibrotic environment. As healthy fetal skin has high levels of TGF-β1, FnEDA, and collagen-III as well, these findings correlate with the in vivo situation. Therefore, our study demonstrates that there are similarities between fetal skin development and fibrosis and shows the necessity to discriminate between these processes.

  8. Cultural Meaning of "Eagle""Dragon"in The Zhi Ge A-Long%探析《支格阿龙》中“鹰”“龙”的文化寓意

    孙子呷呷; 阿育几坡


    毋庸讳言,从生命科学角度讲,因为染色体的原因,“神人”支格阿龙不可能由“鹰或神鹰或神龙鹰”感孕而生;人也不可能由“龙”抚育成长。目前,彝族英雄史诗《支格阿龙》在翻译成汉语时或彝族后代在传承过程中,都把文本中的“dit氐”翻译或解释为“雕或鹰”,这种解读有些偏差。史诗中让濮莫妮依感孕的“鹰”和哺育支格阿龙成长的“龙”分别象征什么?它要向后人表达的是一种怎样的文化寓意?本文从生命科学角度窥探《支格阿龙》,提出“鹰”是男根的象征,“龙”与汉文化相通,是男人的图腾物之观点。以求文献得到更好的保护、传承和解读。%Needless to say, from the viewpoint of life science, because of the chromosome, "god" Zhi Ge A-Long can be impossible gestated by"eagle or condor or dragon eagle";people can not be raised by"dragon". At present, with the Yi heroic epic The Zhi Ge A-Long translated into Chinese or in the transmission process of Yi offspring ,"dit"in the text has been translated or explainted for"eagle or hawk", which understanding isn’t right completely. What do the"eagle"who got PuMonii pregnant and the"dragon"who fostered Zhi Ge A-Long growth symbolize? What cultural meaning does it express to the descendants? From the perspective of life science ,the paper puts forward the"Eagle"is the symbol of male root and"dragon"interlinked Chinese cultures is the totem of man in order to make literature better protection, inheritance and interpretation.

  9. Embodied Cultures of Mobilities

    Jensen, Ole B.


    contemporary theorists understanding bodily movement, material sites of mobility, and social interactions must be consulted along the road (e.g. Latour’s work on objects and ANT, Thrift’s work on the body and ‘non-representational theory, and Massumi’s notions of affects and emotions related to bodily mobility......The paper explores the relationship between the body and mobility by looking into a number of modes of transportation and their ways of constructing particular engagements with mobility. The ‘mobile embodiments’ are significant to a material and symbolic set of relations between human agents...... and material artifacts. The paper target the complex relationship between the moving, sensing body and the material and built environment of infrastructures and mobility modes in order to explore what norms, and meanings, and everyday life mobility cultures are being produced and re-produced in this process...

  10. Meaning Making, Agency, and Languaging in Dialogic Interactions on Academic Writing Tasks: A Sociocultural Discourse Analysis

    Kheradmand Saadi, Zahra


    For Vygotsky, language is a cultural-psychological concept emerged from social interactions and is applied for higher cognitive functions such as thinking, meaning making, and knowledge construction. In this study, a sociocultural perspective was applied to analyze the language produced by 40 sophomore Iranian EFL learners during dialogic…


    Bai Shi


    China's contemporary culture and the protection of its diverse cultural heritage have become some of the most talked about issues today.Cultural prosperity was put forward as an important objective of the Central Government's national development strategy last year.However,the industrialization and commercialization of China's culture have been both criticized and celebrated.Many scholars believe industrialization and enormous government investment may not be the best means to protect intangible cultural heritage (ICH).

  12. Symbolic severance. Charlie or the broken circle of cultural mediation

    Isabelle MATHIEU


    Full Text Available The cultural dimension of the attack on Charlie Hebdo raises questions about the meaning of the event in terms of public policies on art and culture. At issue particulary is the re-situation of those policies in their relationship with democratic achievement in the context of the French republican model. Analysis through the prism of cultural mediation, considered as a communication process designed ti produce a symbolic type of work, casts new light on the objectives and the ways and means of implementing such policies.

  13. Pierre Verger and the construction of african-brazilian’s cultural memory in O Cruzeiro: textual meanings through borders Pierre Verger e a construção da memória cultural afro-brasileira em O Cruzeiro: sentidos textuais através das fronteiras

    Beatriz Marocco


    Full Text Available Based on the concepts of border, culture and memory postulated by Yuri Lotman, this paper aims to analyze Pierre Verger photos in the article titled “Capoeira mata um!”, published on O Cruzeiro magazine. The purpose is to understand the connection of meanings on semiotic boundaries mechanisms in relation to Afro Brazilian culture and memory text constructions in order to contribute to media communication studies. A partir dos conceitos de fronteira, cultura e memória postulados por Yuri Lotman, o objetivo deste artigo é analisar as fotografias de Pierre Verger na matéria “Capoeira mata um!” da revista O Cruzeiro (1948, no intuito de entender a articulação de sentidos nos mecanismos de fronteiras semióticas para a construção de textos da cultura e da memória afrobrasileira, e contribuir para os estudos da Comunicação midiática.

  14. Translation and Culture:Translation as a Cross-cultural Mediation



    Translation is a complex activity which involves language competence as well as proficiency in multiculturalism. From the perspective of multiculturalism, translation resembles recreation of source text by grasping essential meanings to produce a sub-tle target text which can be clearly perceived by target readers. Ignoring cultural issues can present serious mistranslations in the field of advertising translation. This paper aims to explore the significance of connotation confined by the framework of culture and point out that verbal translation is a dangerous inclination by illustrating three business examples. This paper argues that cross-cultural mediation plays an important role in translation.

  15. Chromized Layers Produced on Steel Surface by Means of CVD



    Chemical vapour deposition of chromium on the surface of carbon steel has been investigated using a novel CVD method that combines the low cost of pack cementation method with advantages of vacuum technique. The processes have been performed in chromium chlorides atmosphere at a low pressure range from 1 to 800 hPa, the treatment temperature 800 to 950℃. Studies of the layers thickness, the phase composition, Cr, C and Fe depth profiles in diffusion zone have been conducted. The effect of the vacuum level during the process and the process parameters such as time and temperature on layer diffusion growth on the carbon steel surface has been investigated.

  16. Cultural Analysis - towards cross-cultural understanding

    Gullestrup, Hans

    The book considers intercultural understanding and co-action, partly by means of general insight into concept of culture and the dimensions which bring about cultural differences, and partly as a methodology to analyse a certain culture - whether one's own or others'. This leads towards an unders......The book considers intercultural understanding and co-action, partly by means of general insight into concept of culture and the dimensions which bring about cultural differences, and partly as a methodology to analyse a certain culture - whether one's own or others'. This leads towards...... a theoretical/abstract proposal for cultural understanding. The second part presents a theoretical/abstract proposal for under-standing intercultural plurality and complexity. The third part provides an empirical model for the analysis of intercultural co-action. Finally, the fourth part present and discusses...

  17. Categorizing the meanings of craft: A multi-perspectival framework for eight interrelated meaning categories

    Anna Kouhia


    Full Text Available The meaning and value of crafts as bearers of the cultural heritage are widely acknowledged, and deserved attention has also been paid to the pedagogical applications of craft in practice. Still, research relating to subjectively interpreted meanings of crafts has remained rather scarce. The main interest of the study is to examine subjectively construed meanings of craft associated both to the self-reflective act of making and to the tangible craft object, and categorize the discovered meanings into representative categories. In presenting a multi-perspectival framework for the categories that portray the common kinds of meanings that crafts have, a substance that surpasses cultural boundaries is also captured due to the multicultural research setting. The primary empirical data of the study are composed of interviews of six women from different cultural backgrounds; the supporting data feature a participant observation of a multicultural craft activity group and photographic notes. The wide range of different meanings captured from the data is, during the conducted qualitative content analysis, critically classified into representational categories that typify the personally experienced meanings. The analysis revealed eight interrelated meaning categories regarding the meanings of craft that go beyond cultural boundaries: Meanings proceed from the subjective process of interpreting craft in a socially constructed, personalized setting, but still the meanings given to craft have been given similar characterizations in the several cultural contexts examined. This study adds to an understanding of how personalized meanings contribute to building intercultural reciprocity. Keywords: craft, culture, meanings, interculturality, meaning making

  18. Characterisation of multidrug-resistant Shiga toxin-producing Escherichia coli cultured from pigs in China: co-occurrence of extended-spectrum β-lactamase- and mcr-1-encoding genes on plasmids.

    Bai, Li; Hurley, Daniel; Li, Juan; Meng, Qiong; Wang, Juan; Fanning, Séamus; Xiong, Yanwen


    Identification of Enterobacteriaceae harbouring the plasmid-mediated transferable colistin resistance gene mcr-1 presents a new challenge to public health. The aim of this study was to characterise multidrug-resistant Shiga toxin-producing Escherichia coli (STEC) harbouring the mcr-1 gene on plasmids cultured from pigs in China. Using CHROMagar™ ECC plates combined with stx gene detection by PCR, 93 STEC were recovered from 326 faecal, 351 small intestine content and 326 colon content samples taken from healthy pigs in 2011 and 2012 in China. This study, in which ten colistin-resistant isolates with minimum inhibitory concentrations (MICs) of 8-12 mg/L were identified and found to be positive by PCR for the mcr-1 gene, is a follow-up to an earlier investigation. Plasmid profiling by S1-nuclease digestion followed by pulsed-field gel electrophoresis (PFGE) identified several high-molecular-weight plasmids and these were typed by PCR-based replicon typing (PBRT). Two of the ten isolates, namely STEC-CQ09 (O116:H11/CC23/ST88) and CQ10 (O2:H32/ST3628), were selected for further study as described in this report. Copyright © 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

  19. 汉代听琴典故的文化解读与文学意义%The Cultural Interpretation and Literary Meaning of“Qin-listening”Allusion in Han Dynasty



    听琴故事最早虽可追溯至先秦时期,但在其成为杂剧、戏曲、小说中固定叙事模式的过程中,汉代的听琴典故实为其滥觞。在相如琴挑文君故事中,琴开始用来传递男女之情;而蔡邕听琴惊心的闲笔深意式写法,则对后世叙事文学影响深远。两则听琴典故都为正史所记,而在其流传过程中,典故主人公在后世文学中所被塑造成的不同形象,则由中国古代士阶层的性格特征所决定。听琴不但承载了诸多文化因素,还对后世文学产生了广泛而深远的影响。%Although the story of qin-listening dates back to the pre-Qin period,but in the process of be-coming fixed narrative mode in the novel,drama,opera,the story of qin-listening in the Han Dynasty is the origin .In story of Xiangru incites Wenj un with qin,“qin”started using to pass the love of men and women. The writing of Caiyong’s qin-listening has profound influence on the later literary narrative.Two qin-listen-ing allusions are written in the official,but in the process of circulating,the different images of the allusion heroes in the later literature are determined by the characteristics of Chinese ancient “Shi”class.Qin-listen-ing is not only carried a lot of cultural factors,but also produced extensive and profound literary effect.

  20. 论温泉文化内涵的挖掘——以汝城热水温泉为例%A Study On The Meaning Of Hot Spring Culture Based on Hot Spring in Rucheng, ChenZhou



    This article, on the analysis of the hot spring culture and hot water spring tourist resources, proposes strategies to hot spring culture development, and discusses hot spring culture in the development of tourism.%文章在对温泉文化内涵进行分析的基础上,结合汝城热水温泉的旅游资源特色,对汝城温泉文化内涵的挖掘提出了相应的策略,借以探讨温泉文化在温泉旅游开发中的应用。

  1. Wordplay in Advertising: Form, Meaning and Function

    Sara Laviosa


    Full Text Available Generally speaking, wordplay (or pun is a witticism that relies for its effect on playing with different levels of language, i.e. phonological, graphological, morphological, lexical, syntactic, and textual. Puns are frequently used in commercial advertising as a rhetorical device to promote a given product or service by creating humour, attracting the reader’s attention and adding persuasive force to the message. They also reflect the cultural preferences and traditions of a country, therefore they can be fruitfully used for pedagogic purposes to raise awareness of the specific linguistic and cultural features of the foreign language. In this paper  I examine the form, meaning and function of puns that rely on the different meanings of polysemic words, the literal and non-literal senses of idioms or on bringing two homonyms together in the same utterance to produce witty remarks. After introducing the notions of homonymy, polysemy and idiom I analyse the play on words contained in a sample of advertisements selected at random from two broadsheet newspapers, The Guardian Weekend (1997 and The Independent (1997, two quality weekly magazines, Cosmopolitan (1997 and The Telegraph Magazine (1997, as well as a promotional brochure by Alliance & Leicester plc (2003 and promotional leaflets published in 2000 respectively by Royal Mail, British Telecommunications plc and Johnson & Johnson. Finally, I propose some activities that can be carried out in the LSP classroom either individually or in groups to raise awareness of some of the linguistic and cultural features characterizing the rhetoric of marketing and promotion in business language.

  2. "Fraqueza de nascença": sentidos e significados culturais de impressões maternas na saúde infantil no Nordeste brasileiro "Birth weakness": cultural meanings of maternal impressions for infant health in Northeast Brazil

    Paola Gondim Calvasina


    Full Text Available A gestação abrange dimensões sócio-culturais, históricas e afetivas, que processam no corpo das mulheres diversos sentidos e significados. Cada sociedade constrói concepções, práticas ou modelos explicativos populares, que se diferenciam do modelo biomédico e visam a proteger a mãe-feto, promovendo uma gravidez saudável. Esta pesquisa qualitativa, baseada na antropologia interpretativa, desvela as experiências vivenciadas por 27 mulheres pobres e suas repercussões na desnutrição dos filhos atendidos num Centro de Desnutrição Infantil em Fortaleza, Nordeste brasileiro. De janeiro a junho de 2004, foram realizadas entrevistas etnográficas e narrativas sobre fraqueza de nascença e observação-participante de cuidados no ambulatório, enfermaria e domicílios. Conforme se argumenta, as mães acreditam que seus sofrimentos físicos, emocionais e a precária condição nutricional é "impressa" no feto, resultando na desnutrição do filho. Enquanto a etnoetiologia da "fraqueza" aponta fatores externos do corpo que involuntariamente atingem a gestante, a visão médica relatada tende a culpabilizar a mãe. É preciso compreender a narrativa das mães, sensibilizar-se com o seu sofrimento e aproximar as concepções populares e biomédicas.Pregnancy encompasses socio-cultural, historical, and affective dimensions that process various meanings in women's bodies. Each society constructs popular concepts, practices, and explanatory models that differ from the biomedical model and aim to protect the mother and fetus and foster a healthy pregnancy. This qualitative study, based on interpretative anthropology, unveils the experiences of 27 poor women and their repercussions on the malnutrition of their infants, treated at a Childhood Malnutrition Treatment Center in Fortaleza, Northeast Brazil. From January to June 2004, ethnographic and narrative interviews were conducted on so-called "birth weakness", in addition to participant

  3. Theories of Culture



      “Culture” is such a broad concept which is understood and defined differently by different people and has been remaining a focus for research. Some view culture as skills, values, understandings, knowledge or ways of being achieved as members of society and it is acquired and transmitted over generations; some regard culture as meaning which is established and constructed in practice and it is the context of production of new meaning and constraint of action. In this article it will focus on two theories of culture, namely, Cultural Relativism and Cultural Structuralism, and will illustrate the general ideas, main representatives and their arguments of these two theories.

  4. Significados e sentidos da música: uma breve "composição" a partir da psicologia histórico-cultural Significados y sentidos de la música: una breve "composición" a partir de la psicología histórico-cultural Meanings and senses of music: a brief composition from cultural-historical psychology

    Patrícia Wazlawick; Denise de Camargo; Kátia Maheirie


    Este artigo discute a construção dos significados e sentidos da música a partir da Psicologia Histórico-Cultural, principalmente com a contribuição de L. S. Vygotski, buscando tecer uma interface com as idéias dos musicoterapeutas noruegueses Even Ruud e Brynjulf Stige, com base na filosofia de Ludwig Wittgenstein. Quando se vivencia a música, não se estabelece relação apenas com a matéria musical em si, mas com toda uma rede de significados construídos no mundo social, em contextos coletivos...

  5. Cultural Identity Through CLIL

    Oprescu Monica


    Full Text Available The CLIL approach is a modern manner of teaching English, which has been adapted in Romanian schools and universities. An interesting aspect of learning a foreign language is the contact with its culture/s and the changes it produces in terms of identity. Therefore, a challenging question to be answered is whether a CLIL approach focusing on culture influences students' cultural identity.

  6. Biological Control of Aphid Using Fungal Culture and Culture Filtrates of Beauveria bassiana.

    Kim, Jeong Jun; Jeong, Gayoung; Han, Ji Hee; Lee, Sangyeob


    Aphids are one of the most destructive pests in crop production such as pepper, cucumber, and eggplants. The importance of entomopathogenic fungi as alternative pest control agents is increasing. Conidia of entomopathogenic fungi are influenced by environmental conditions, such as temperature and relative humidity, and cause slow and fluctuating mortality. These factors have prevented wider application and use of biocontrol agents. For investigation of means of mitigation of such problems, we conducted bioassays with 47 fungal culture filtrates in order to evaluate the potential of secondary metabolites produced by entomopathogenic fungi for use in aphid control. Among 47 culture filtrates cultured potato dextrose broth, filtrate of Beauveria bassiana Bb08 showed the highest mortality (78%) against green peach aphid three days after treatments. Filtrate of Bb08 cultured in Adamek's medium showed higher toxicity as 100% to third instar nymphs of the aphid compared with seven other filtrates cultured in different broths amended with colloidal chitin or oil. The culture filtrates and fungal cultures from media amended with colloidal chitin or oil had lower control efficacies than filtrates without these additives in three different media. These results indicate that the fungal culture fluid or culture filtrate of B. bassiana Bb08 cultured in Adamek's medium has potential for development as a mycopesticide for aphid control.

  7. Culture and social class.

    Miyamoto, Yuri


    A large body of research in Western cultures has demonstrated the psychological and health effects of social class. This review outlines a cultural psychological approach to social stratification by comparing psychological and health manifestations of social class across Western and East Asian cultures. These comparisons suggest that cultural meaning systems shape how people make meaning and respond to material/structural conditions associated with social class, thereby leading to culturally divergent manifestations of social class. Specifically, unlike their counterparts in Western cultures, individuals of high social class in East Asian cultures tend to show high conformity and other-orientated psychological attributes. In addition, cultures differ in how social class impacts health (i.e. on which bases, through which pathways, and to what extent). Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. 产细菌纤维素菌株中间葡糖醋杆菌的分离与发酵条件优化%Isolation and Culture Optimization of Bacterial Cellulose Producing Strain Gluconacetobacter intermedius

    苏俊霞; 陆震鸣; 王宗敏; 史劲松; 陆茂林; 许正宏


    Five bacterial cellulose (BC) producing strains were isolated from solid -state fermentation cultures of traditional Chinese vinegar,and identified as Gluconacetobacter intermedius based on their physiological and biochemical characteristics as well as 16S rDNA sequence analysis. Strain 1-17 could produce more BC then the others. Furthermore,surface features and chemical structure of BC were analyzed by scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy,respectively. Effects of temperature,time,carbon sources and initial pH on BC production were tested. The optimal temperature for strain 1-17 was 35 ℃, and the fermentation time was 7 d. Glucose and glycerol were the optimal carbon sources,and the optimal initial pH was 6.0. Both lactate and calcium could promote the synthesis of BC. The yield of BC was improved from (3.90±0.08) g/L to (7.90±0.19) g/L under the optimal conditions.%从中国传统固态发酵食醋醋醅中分离出5株产细菌纤维素(BC)的菌株,经生理生化特征及16S rDNA序列分析,它们均属于中间葡糖醋杆菌(Gluconacetobacter intermedius),其中编号为1-17的菌株初始产量较高。应用扫描电镜技术(SEM)和傅里叶红外光谱技术(FT-IR)分析了BC结构特征。采用单因素研究了温度、培养时间、碳源、初始pH对BC合成的影响。确定菌株1-17最适温度为35℃,发酵时间为7 d,甘油和葡萄糖为最适碳源,最适初始pH为6.0,乳酸根离子和钙离子能够促进BC的合成。通过培养条件优化使得细菌纤维素产量从初始的(3.90±0.08) g/L增加到(7.90±0.19) g/L。

  9. Isolation of biosurfactant producing microorganisms and lipases from wastewaters from slaughterhouses and soils contaminated with hydrocarbons

    Becerra, Lizzie; Horna, María


    Surfactants are amphipathic molecules which reduce stress at the interface, thereby increasing water solubility and availability of organic compounds are produced by bacteria, fungi, and yeasts. For the isolation of biosurfactant producing bacteria and lipases, was plant in inducing means 10% of sewage effluent from slaughterhouses and soils contaminated with hydrocarbons Province taps Trujillo - Peru. Isolates were seed in agar cultures lecithin and rhodamine agar for determination of lipase...

  10. 泰国銮披汶时期的文化政策及其意涵%The Cultural Policy and Its Meaning during the Phibunsongkhram Period of Thailand



    In 1938 after Phibunsongkhram became Prime Minister of Thailand, he vigorously promoted extremist nationalist policies, which were largely based on a top-down cultural transformation implemented by way of national legislation, also known as"cultural nationalism". It had a profound impact on Thai art and culture and social development in many aspects, and played an important role in shaping modern Thai identity and culture. His cultural transformation took western society as a standard and hoped that Thailand, as a nation-state, could reach the level of civilization of Western countries, which was related to his awareness of cultural pragmatism. Reviewing Phibunsongkhram's cultural policy and analyzing its implication will help us better understand his nationalist policies, as well as the foundation of modern Thai culture. It should be recognized that the transformed Thai culture with a typical western style still preserves"Thainess"in nature.%銮披汶1938年出任泰国总理后大力推行极端民族主义政策,其中不少是通过自上而下的文化改造国家法令推行的,因此也被称为“文化民族主义”。这一文化政策对泰国的艺术文化、社会发展等方面都产生了深刻的影响,在泰国现代社会的身份认同和文化塑造方面也发挥了重要作用。銮披汶时期的文化改造以西方社会作为模板,以作为民族国家的泰国能达到西方国家文明水准为目标,这与他对文化实用主义的认知有关。梳理并剖析泰国銮披汶时期的文化政策,将有助于我们更好地理解其民族主义政策及泰国现代文化的生成根基。

  11. Construction of Hong-dae cultural district : cultural place, cultural policy and cultural politics

    Cho, Mihye


    This dissertation examines how the process of creating the "Hong-dae cultural district" in Seoul has involved the mobilisation of various social groups and triggered the (re)institutionalisation of the meaning of "the cultural". It seeks to explicate how a cultural policy project can stimulate the emergence of social groups, which challenge existing policy provisions and laws and lead to the (re)institutionalisation of "Hong-dae culture". In so doing, the author will be able to simultaneously...

  12. Estudo da cultura canavieira na região de Piracicaba por fotointerpretação: Parte II A study on sugarcane cultures in the Piracicaba region by means of photointerpretation: Part II

    Raul Audi


    Full Text Available Dando prosseguimento ao Estudo da Cultura Canavieira na região de Piracicaba, são apresentadas, com base no estudo de fotografias aéreas, as principais características dessa cultura no ano de 1962. Considerando-se os municípios de Rio das Pedras, Mombuca, Capivari e Rafard, foram determinados os dados de áreas cultivadas, localização das culturas e usinas, aspectos das culturas e dos terrenos empregados.Pursuing research work about sugarcane cultures in the region of Piracicaba, their main characteristics were studied in this paper, for the year 1962, based on aerial photographs. Taking into consideration such districts as Rio das Pedras, Mombuca, Capivari and Rafard, a large number of data was determined, of areas under cultivation, localization of cultures and factories, aspects of cultures and of lands used for this crop.

  13. The dance of meaning

    Rasmussen, Ole Elstrup


    competence, qualifications, sense making, reasoning, meaning, intentionality, interpersonal relationship......competence, qualifications, sense making, reasoning, meaning, intentionality, interpersonal relationship...




    Full Text Available Cultural institution management is to direct the organization to a specific cultural profile purpose, namely production values esthetic sense, artistic, moral, spiritual, distribution, promotion of these values, protection and circulation of cultural heritage. In this regard, an analysis in the diagnosis cultural organizations aims to determine the main strengths and weaknesses, assess the potential and making recommendations focused on the root causes of failures and positive aspects. This paper presents considerations diligence activity Culture House "IL Caragiale "in Ploiesti. The mission of this organization is to contribute to the cultural development of the community by initiating projects and cultural programs, offer development programs and services to meet cultural needs, increase public access to diverse cultural life, providing a constant presence institution circuit local, national, European and international level. Conclusions drawn from the analysis shows that in a world of economic globalization, information and culture in a company in constant change, in a competitive market where there is information readily available means and leisure, but not cultural consistency in a social environment where interest in culture of people is declining, the situation of the population is impaired, the remuneration of staff working in the field of cultural education is demotivating, the funds allocated to culture have grown lately effectively lead a cultural institution is a challenge.


    Felipe Oliveira CARIONI


    industry, being directly related to the final product flavor, texture, colour and shelf life. The use of starter cultures to produce fermented sausages with a base of duck meat from the boning of thighs and real thighs was evaluated in this research. A mixture of Lactobacillus plantarum BN and Kokuria varians CCT 4492 starter cultures was used to inoculate the meat mixture. The sausages were smoked in a smoking chamber at 23±1°C for approximately 19 hours and were cured for 25 days. The initial counting of viable cells in the sausage mixture was 6.08Log10 CFU/g and 6.04Log10 CFU/g for lactic acid bacteria and Micrococcacea, respectively. Acid lactic bacteria displayed a growth of 0.79 logarithm cycles after the second day of processing, and an increase of 2.58 logarithm cycles at the 11th day. The final product acidity average value in lactic acid was 0,39%, and the pH value, 5.11. The physico-chemical analyses rested within Brazilian Legal Standards. The final product presented a sensorial profile within acceptable quality standards.

  16. Optimize culture conditions of streptococcus thermophilus for fermentation producing nisin by response surface methodology%响应面法优化 Nisin 发酵的培养条件

    贺小贤; 郑茂奎; 刘欢; 彭光杰


    In order to improve fermentation titer unit of Nisin ,the MRS was used as the fer‐mentation medium by streptococcus thermophilus and titer unit of Nisin to Staphylococcus aureus which is used as indicator bacteria serve as detection index of titer unit of Nisin .On the basis of single factor experiment ,optimization of fermentation initial pH ,inoculation a‐mount of Streptococcus thermophilus and corn steep liquor content for producing Nisin by u‐sing response surface analysis methodology .The experiment results showed that the optimal fermentation condition was initial pH 6 .60 ,inoculation amount of 4 .80% and corn steep liq‐uor content of 25 .60% ,and corn steep liquor content is the maximum factor for affecting the fermentation titer unit of Nisin and inoculation is the minimal factor .Under these conditions , the Nisin titer unit was increased by 18 .07% and reached up to 1 602 .77 ± 6 .38 IU/mL which is consistent with the predicted value .These show that it is effective for optimizing culture conditions of Nisin by response surface methodology .%为了提高嗜热乳链球菌发酵生产Nisin的效价,以M RS为发酵培养基,以金黄色葡萄球菌为效价检测指示菌,以Nisin效价为考察指标,在单因素实验的基础上,采用响应面分析法优化发酵初始pH、接种量以及玉米浆添加量.结果表明影响Nisin效价的最大因素是玉米浆添加量,其次是发酵初始p H ,接种量影响最小.最佳初始p H6.60,接种量4.80%,玉米浆添加量2560%,在此条件下 Nisin 的效价可达1602.77±6.38 IU/mL ,较优化前提高了18.07%,其实验结果与模型预测值拟合良好,说明通过响应面试验设计对Nisin发酵条件的优化是有效的.

  17. 邓小平军队文化工作理论的深刻内涵与现实意义%In-depth Connotations and Practical Meanings of Deng Xiaoping’s Theory about Military Cultural Work



    从邓小平文艺理论、邓小平思想教育理论、邓小平军队政治工作理论三个方面分析邓小平军队文化工作理论的理论渊源,认为邓小平新时期军队文化工作理论的科学内涵是军队文化工作的重要性、继承性、人民性和政治性。邓小平军队文化工作理论具有三个方面的启示:一是军队文化工作是贯彻党的方针政策、占领思想文化阵地、加强军队全面建设的需要,是鼓舞士气、凝聚力量、提高战斗力的需要,是优化军营环境、服务官兵、活跃军营的需要;二是军队文化工作必须在坚持正确方向、弘扬主旋律、营造文化氛围、创作精品方面,做到思想性与娱乐性、战斗性与抒情性、群众性与专业性的统一;三是军队文化工作要与价值观培育、官兵兴趣爱好、求知成才、先进文化接轨。%This thesis analyses the theoretic roots of Deng Xiaoping’s military cultural work theory from three aspects:Deng Xiaoping’s theory about literature and art ,ideological education and military politics . Deng Xiaoping’s military culture work theory in the new period has connotations of importance ,legacy , people and politics .Deng Xiaoping’s military cultural work theory has three inspirations .First ,military cultural work is needed for implementation of the Party’s guidelines and policies ,occupying the ideological and cultural front and strengthening comprehensive military development ;for encouraging the morale , gathering strength and improving the fighting capacity and for optimizing the military environment ,ser‐ving the officers and soldiers and activating the military camps .Secondly ,w hile maintaining the correct di‐rection ,carrying forward the central theme ,building cultural atmosphere and creating masterpieces ,we should achieve the unity of ideology and entertainment ,combat and emotion ,popularity and professionali‐ty .Thirdly ,cultural work

  18. Culture-sensitive psychotraumatology

    Ulrich Schnyder


    Full Text Available Background: Although there is some evidence of the posttraumatic stress disorder (PTSD construct's cross cultural validity, trauma-related disorders may vary across cultures, and the same may be true for treatments that address such conditions. Experienced therapists tailor psychotherapy to each patient's particular situation, to the nature of the patient's psychopathology, to the stage of therapy, and so on. In addition, culture-sensitive psychotherapists try to understand how culture enhances the meaning of their patient's life history, the cultural components of their illness and help-seeking behaviors, as well as their expectations with regard to treatment. We cannot take for granted that all treatment-seeking trauma survivors speak our language or share our cultural values. Therefore, we need to increase our cultural competencies. Methods: The authors of this article are clinicians and/or researchers from across the globe, working with trauma survivors in various settings. Each author focused on one or more specific cultural aspects of working with trauma survivors and highlighted the following aspects. Results: As a result of culture-specific individual and collective meanings linked to trauma and trauma-related disorders survivors may be exposed to (self-stigma in the aftermath of trauma. Patients who are reluctant to talk about their traumatic experiences may instead be willing to write or use other ways of accessing the painful memories such as drawing. In other cultures, community and family cohesion are crucial elements of recovery. While awareness of culture-specific aspects is important, we also need to beware of premature cultural stereotyping. When disseminating empirically supported psychotherapies for PTSD across cultures, a number of additional challenges need to be taken into account: many low and middle income countries have very limited resources available and suffer from a poor health infrastructure. Conclusions: In summary

  19. Determination of free amino acids in whole-fat Turkish White Brined Cheese produced by animal and microbial milk-clotting enzymes with and without the addition of starter culture

    Ufuk Eren-Vapur; Tulay Ozcan


    Coagulating enzymes are essential ingredients for the production of different cheese varieties. The objective of this research was to summarize the effect of rennet type (calf rennet and microbial rennet from Rhizomucor miehei) and starter culture on the sensory properties and free amino acids (FAA) release during the ripening of Turkish White brined cheese. The concentrations of FAA and sensory properties were similar for cheeses made with both types of coagulant and starter culture. Aminoac...

  20. An Optimized Method for Suspension Culture of CHO Cells to Produce Recombinant Human Erythropoietin (EPO)%悬浮培养CHO细胞生产重组人促红细胞生成素条件的优化

    杨栋; 牛红军; 陆刚; 史嘉林; 孙浩明; 李晖


    Objective: To screen and domesticate the adherent cultured CHO cells to obtain high expression of cell suspension culture for production of recombinant human erythropoietin erythropoietin (rHuEPO). Methods: Using 96-well and 24-well plates culture method to screen and domesticate the highly expressing CHO cell strain. Acclimate the high expression cell strain and make it suitable for suspension culture. It's inoculated into the bioreactor in serum-free culture after amplified by the shake flask, and monitoring of glucose content, measuring rHuEPO expression of daily. Results: The suspension culture of CHO cell production of rHuEPO has short production period, higher expression than adherent culture. On the other hand, it is easy to operate and scale-up, but not easy to pollute. Furthermore, we established of the CHO cell strain for suspension culture,which provided a technical basis for industrial production of CHO cells the rHuEPO. Conclusion: After process optimization, the use of serum-free suspension culture production of erythropoietin average expression has high, short production period, low cost of production.than adherent culture.%目的:通过对贴壁培养CHO细胞筛选驯化,得到高表达的细胞后进行悬浮培养生产重组人促红细胞生成素(rHuEPO).方法:利用96孔板和24孔板对CHO细胞进行筛选,得到高表达细胞株后进行驯化,使其适合悬浮培养,经过摇瓶扩增后接种到生物反应器中无血清培养,每天监测葡萄糖含量,测rHuEPO表达量.结果:悬浮培养CHO细胞生产rHuEPO,生产周期短,表达量比贴壁培养高出很多,操作方便,减少污染,易于放大,并建立了适合悬浮培养的CHO细胞株,为工业化悬浮培养CHO细胞生产rHuEPO提供了技术基础.结论:经过工艺优化后利用无血清悬浮培养生产促红细胞生成素平均表达量较贴壁培养高,生产周期短,有利于降低生产成本.

  1. Making meaning(s with cover designs

    Yvonne Reed


    Full Text Available In this article Halliday’s systemic functional grammar, Kress and Van Leeuwen’s grammar of visual design and Bernstein’s work on pedagogic discourse are drawn on in order to analyze some of the ways in which meanings are made and some meanings privileged over others in the designing, both visual and discursive, of the covers of three course books prepared for teacher education programmes in South Africa. The primary aim of this analysis is to understand what meanings are offered to readers as entrants into or as established members of communities of practice in the teaching profession and the academy.

  2. Time Reference in Different Cultures.

    Khairullin, Vladimir


    Discusses time references in Russian- and English-speaking cultures by means of Russian translation variants of works by twentieth-century English-language writers. Suggests the different attitudes toward time as manifested by these two distinct cultures. (HB)

  3. Defining moments : a cultural biography of Jane Eyre


    This thesis examines the ways in which various practices, such as novel-writing, publishing, book-reviewing, reading for pleasure, adaptation and studying English literature, have produced Jane Eyre’s complex cultural profile. The organizing principle of the study is Paul du Gay, Stuart Hall et al’s ‘circuit of culture’, which identifies five key processes or ‘moments’ as being productive of the meanings that a cultural artefact or text comes to possess. Explaining the meanings which have bee...

  4. Kinetics of High Cell Density Fed-batch Culture of Recombinant Escherichia coli Producing Human-like Collagen%重组大肠杆菌分批-补料高密度发酵生产类人胶原蛋白的动力学

    花秀夫; 范代娣; 骆艳娥; 张兮; 施惠娟; 米钰; 马晓轩; 尚龙安; 赵桂仿


    The kinetics of batch and fed-batch cultures of recombinant Escherichia coli producing human-like collagen was investigated. In the batch culture, a kinetic model of a simple growth-association system was concluded without consideration of cell endogeneous metabolism. The cell lag time, the maximum specific growth rate and growth rates were set at (0.15, 0.2, 0.25h-1) by the method of pseudo-exponential feeding, and the expressions for the specific rate of substrate consumption, the growth kinetics and the product formation kinetics of each phase spectively. The model predictions are in good agreement with the experimental data.

  5. Active causation and the origin of meaning

    van Hateren, J.H.


    Purpose and meaning are necessary concepts for understanding mind and culture, but appear to be absent from the physical world and are not part of the explanatory framework of the natural sciences. Understanding how meaning (in the broad sense of the term) could arise from a physical world has prove

  6. Cultural Meanings, Inheritance of Toponymy——A Study on Shanghai Toponymy Planning System%文化聚力,地名传承——上海地名规划体系的探讨

    许菁芸; 夏丽萍


      地名具有重要的实用指位价值和历史文化价值。在剖析城市地名管理中存在的文化性、规范性、时序性问题的基础上,借鉴国内相关城市地名规划的经验,提出构建上海“分类型、分层级”的地名规划体系的设想,倡导地名规划与城市规划紧密结合、有效互动、同步推进。%  Toponymy has geographic significance and contains simultaneously cultural and historical values. This article considers the cultural, ordinary and temporal characters of the urban toponymy administration, it studies the relevant experiences of the urban toponymy plan of suitable Chinese cities and proposes a“categorized, hierarchical”toponymy plan system for Shanghai. It suggests efficient integration, interaction and coupled development of urban toponymy and urban planning process.

  7. Experiencing Global Culture in Vatel

    Kampf, Constance Elizabeth; Broillet, Alexandra; John, Claudette


    of reference by offering a global culture frame, drawing on cultural resources from country based “settled” cultural contexts, and reflects on how global cultural contexts can benefit from a knowledge management approach to this dynamic between “settled” cultural contexts and “unsettled” cultural ideologies......To explore the meaning of “global culture” in a professional communication context, this paper explores the “unsettled” global culture of Vatel, a private business school educating students from nearly 50 different countries for the hospitality industry. This paper explores the role of knowledge...... management in understanding global culture, arguing that the notion of “unsettled” cultures demonstrates how ideologies function in global settings and draw on national “settled” cultural resources. In unpacking different aspects of Vatel's culture this paper questions assumptions built into cultural frames...

  8. Determination of free amino acids in whole-fat Turkish White Brined Cheese produced by animal and microbial milk-clotting enzymes with and without the addition of starter culture

    Ufuk Eren-Vapur


    Full Text Available Coagulating enzymes are essential ingredients for the production of different cheese varieties. The objective of this research was to summarize the effect of rennet type (calf rennet and microbial rennet from Rhizomucor miehei and starter culture on the sensory properties and free amino acids (FAA release during the ripening of Turkish White brined cheese. The concentrations of FAA and sensory properties were similar for cheeses made with both types of coagulant and starter culture. Aminoacids Phe, Leu - Ile, Gln, Val, Pro and Ala were the principal FAAs in the White brined cheeses at all stages of ripening.

  9. Monoterpenes produced by Cupressus lusitanica cultured cells including a novel monoterpene (1R, 2S, 6S)-(+)-1,6-epoxy-4(8)-p-menthen-2-ol [corrected].

    Matsunaga, Yoko; Fujita, Koki; Yamada, Junko; Ashitani, Tatsuya; Sakai, Kokki


    A novel monoterpene,(1R, 2S, 6S)-(+)-1,6-epoxy-4(8)-p-menthen-2-ol [corrected], was isolated from an elicitor-treated cell culture of Cupressus lusitanica (Mexican cypress). Ten known monoterpenes--limonene, myrcene, beta-ocimene, sabinene, terpinolene, 4-terpineol, alpha-terpineol, 4(8)-p-menthen-1,2-diol, 4-hydroxyphellandric acid methylester and beta-thujaplicin methylether--were also identified. Regioselective metabolisms of monoterpenes were observed in this culture.

  10. 7 CFR 1207.305 - Producer.


    ... Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan Definitions § 1207.305 Producer. Producer means any person engaged...

  11. Cerebrospinal fluid (CSF) culture

    ... is a laboratory test to look for bacteria, fungi, and viruses in the fluid that moves in ... culture medium. Laboratory staff then observe if bacteria, fungi, or viruses grow in the dish. Growth means ...

  12. Dialysis cultures.

    Pörtner, R; Märkl, H


    Dialysis techniques are discussed as a means for effective removal of low-molecular-mass components from fermentation broth to reach high cell density. Reactor systems and process strategies, the relevant properties of membranes and examples for high-density fermentation with dialysis, and problems related to scale-up are addressed. The dialysis technique has turned out to be very efficient and reliable for obtaining high cell densities. As in dialysis processes the membranes are not perfused, membrane clogging is not a problem as it is for micro- and ultrafiltration. By applying a "nutrient-split" feeding strategy, the loss of nutrients can be avoided and the medium is used very efficiently. The potential of dialysis cultures is demonstrated on the laboratory scale in a membrane dialysis reactor with an integrated membrane and in reactor systems with an external dialysis loop. In dialysis cultures with different microorganisms (Staphylococci, Escherichia coli, extremophilic microorganisms, Lactobacilli) the cell densities achieved were up to 30 times higher than those of other fermentation methods. The technique enables high cell densities to be attained without time-consuming medium optimization. For animal cell cultures the concept of a fixed bed coupled with dialysis proved to be very effective.

  13. Internalized Meaning Factualism

    Hohwy, Jakob


    are defended by, e.g., Robert Brandom, Paul Horwich and Huw Price), that promises to solve the problems. According to this position, the problem of meaning can be solved by prohibiting an external perspective on meaning constituting properties. The idea is that if we stick to a perspective on meaning......The normative character of meaning creates deep problems for the attempt to give a reductive explanation of the constitution of meaning. I identify and critically examine an increasingly popular Carnap-style position, which I call Internalized Meaning Factualism (versions of which I argue...... that is internal to meaning discourse, then we can preserve the normativity of meaning and yet locate meaning in the natural world. I develop a generic motivation for this position, but argue that, since this motivation is consistent with the Ramsey-Carnap-Lewis-Jackson reductionist strategy, internalized meaning...

  14. Cultural Diplomacy in Europe.

    Haigh, Anthony

    The evolution of European government activities in the sphere of international cultural relations is examined. Section 1 describes the period between World War I and World War II when European governments tried to enhance their prestige and policies by means of cultural propaganda. Section 2 analyzes the period during World War II when the…

  15. Teaching Languages, Teaching Cultures.

    Liddicoat, Anthony J., Ed.; Crozet, Chantal, Ed.

    This collection of papers examines what it means to teach culture as an integrated part of language from both the language learner's and the language teacher's perspectives. The 11 papers include the following: "Teaching Cultures as an Integrated Part of Language: Implications for the Aims, Approaches and Pedagogies of Language Teaching"…

  16. Conceptualizing leadership across cultures

    M.W. Dickson; N. Castaño; A. Magomaeva; D.N. den Hartog


    In this article, we summarize research on how the meaning of leadership varies systematically across cultures, and describe the conflict in the literature between the quest for universals and the identification of cultural contingencies in leadership theory. We review the literature on the relations

  17. Cultural Meaning of Nijiaochui in Huaibin and the Mud Dog in Huaiyang%淮滨泥叫吹与淮阳泥泥狗的文化意蕴变迁



    淮滨泥叫吹和淮阳泥泥狗都是独具地域特点的民间泥塑工艺品。据考证,淮滨和淮阳都属于古淮夷,同属以太阳崇拜和鸟图腾崇拜的原始部落的东夷两昊集团,二者同根同源。但是,当代的淮滨泥叫吹的创作与早期龙山文化的代表沙冢长尾红陶鸟一脉相承,形似神似,极具原始性;当代淮阳泥泥狗的创作形式及其所蕴含的文化内涵发生了较大的改变。%The Nijiaochui in Huaibin and the Mud Dog in Huaiyang are both the clay sculpture artwares with very special national fea-tures.According to research, Huaibin and Huaiyang both belong to the ancient Huaiyi area, included in Dongyi and Liangyu groups in primitive tribes which used the sun and bird totem as their worship.But, the creation of contemporary Nijiaochui in Huaibin and sand mound nagao terracotta birds as the representative in Early Longshan culture are from the same strain, which are primitive, quite simi-lar in form and spirit.However, the creative forms and cultural connotation have had a greater change in contemporary Mud Dog in Huaiyang.

  18. Politics of Meaning in Mathematics Education

    Skovsmose, Ole


    By a politics of meaning I refer to the social, economic, cultural and religious conditions for experiencing meaning. I refer as well to the layers of visons, assumptions, presumptions and preconceptions that might construct something as being meaningful. By addressing different politics of meani...... of such factors. Politics of meaning can be analysed with reference to sexism, racism, instrumentalism, the school mathematics tradition, critical mathematics education, and the banality of expertise....

  19. Geometric Mean--What Does It Mean?

    Kalder, Robin S.


    The National Council of Teachers of Mathematics and numerous mathematics educators promote the combination of conceptual understanding and procedural learning in the successful instruction of mathematics. Despite this, when geometric mean is taught in a typical American geometry class, it is taught as a process only despite the many connections…

  20. Oscillating Cell Culture Bioreactor

    Freed, Lisa E.; Cheng, Mingyu; Moretti, Matteo G.


    To better exploit the principles of gas transport and mass transport during the processes of cell seeding of 3D scaffolds and in vitro culture of 3D tissue engineered constructs, the oscillatory cell culture bioreactor provides a flow of cell suspensions and culture media directly through a porous 3D scaffold (during cell seeding) and a 3D construct (during subsequent cultivation) within a highly gas-permeable closed-loop tube. This design is simple, modular, and flexible, and its component parts are easy to assemble and operate, and are inexpensive. Chamber volume can be very low, but can be easily scaled up. This innovation is well suited to work with different biological specimens, particularly with cells having high oxygen requirements and/or shear sensitivity, and different scaffold structures and dimensions. The closed-loop changer is highly gas permeable to allow efficient gas exchange during the cell seeding/culturing process. A porous scaffold, which may be seeded with cells, is fixed by means of a scaffold holder to the chamber wall with scaffold/construct orientation with respect to the chamber determined by the geometry of the scaffold holder. A fluid, with/without biological specimens, is added to the chamber such that all, or most, of the air is displaced (i.e., with or without an enclosed air bubble). Motion is applied to the chamber within a controlled environment (e.g., oscillatory motion within a humidified 37 C incubator). Movement of the chamber induces relative motion of the scaffold/construct with respect to the fluid. In case the fluid is a cell suspension, cells will come into contact with the scaffold and eventually adhere to it. Alternatively, cells can be seeded on scaffolds by gel entrapment prior to bioreactor cultivation. Subsequently, the oscillatory cell culture bioreactor will provide efficient gas exchange (i.e., of oxygen and carbon dioxide, as required for viability of metabolically active cells) and controlled levels of fluid