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Sample records for convalescence

  1. Convalescence after inguinal herniorrhaphy

    DEFF Research Database (Denmark)

    Bay-Nielsen, M; Thomsen, H; Andersen, F Heidemann;

    2004-01-01

    BACKGROUND: Convalescence after inguinal herniorrhaphy is usually 3-4 weeks and is an important outcome parameter of hernia surgery. The aim of this study was to describe in detail the consequences of recommending a short convalescence, including the risk of recurrence. METHODS: This was a multic......BACKGROUND: Convalescence after inguinal herniorrhaphy is usually 3-4 weeks and is an important outcome parameter of hernia surgery. The aim of this study was to describe in detail the consequences of recommending a short convalescence, including the risk of recurrence. METHODS......) was compared with that for comparable patients treated in participating departments but not part of the study group (group 2, n = 1306) and patients in the Danish Hernia Database (group 3, n = 8297). RESULTS: The median time off work was 7 days and the time interval before carrying out the most strenuous...... (approximately 20 per cent). The reoperation rate in group 1 at the median observation time was 0.7 per cent, which was no different to that in group 2 (1.6 per cent) (P = 0.186) or group 3 (1.4 per cent) (P = 0.092). CONCLUSION: Reduced convalescence after inguinal herniorrhaphy may be recommended without...

  2. Convalescence after laparoscopic inguinal hernia repair

    DEFF Research Database (Denmark)

    Tolver, Mette Astrup; Rosenberg, Jacob; Bisgaard, Thue

    2016-01-01

    BACKGROUND: Duration of convalescence after inguinal hernia repair is of major socio-economic interest and an often reported outcome measure. The primary aim was to perform a critical analysis of duration of convalescence from work and activity and secondary to identify risk factors for unexpected...... prolonged convalescence after laparoscopic inguinal hernia repair. METHODS: A qualitative systematic review was conducted. PubMed, Embase and the Cochrane database were searched for trials reporting convalescence after laparoscopic inguinal hernia repair in the period from January 1990 to January 2016...... factors for prolonged convalescence extending more than a few days after laparoscopic inguinal hernia repair. CONCLUSIONS: Patients should be recommended a duration of 1-2 days of convalescence after laparoscopic inguinal hernia repair. Short and non-restrictive recommendations may reduce duration...

  3. Pain and convalescence after laparoscopic cholecystectomy

    DEFF Research Database (Denmark)

    Bisgaard, T; Kehlet, H; Rosenberg, J

    2001-01-01

    Pain and speed of convalescence are the two main problems after uncomplicated laparoscopic cholecystectomy. We therefore identified interventional and descriptive studies in electronic databases and supplemented them with manual searches. Pain and interventional analgesic studies were analysed......, the use of intraperitoneal local anaesthetics, and the type of general anaesthesia. Pain and medico-cultural traditions are the main factors responsible for prolonged convalescence after laparoscopic cholecystectomy. To minimise pain and the duration of convalescence, we recommend multi-modal analgesic...... treatment in combination with short, standardised instructions to resume work and normal activity....

  4. Short convalescence after vaginal prolapse surgery

    DEFF Research Database (Denmark)

    Ottesen, Marianne; Sørensen, Mette; Kehlet, Henrik

    2003-01-01

    , 0-24) after first-time prolapse surgery. In the prospective study, there were 41 consecutive women with a median age of 69 years (range, 44-88). Convalescence was median intercourse, sports and work with lifts...

  5. Short convalescence after vaginal prolapse surgery

    DEFF Research Database (Denmark)

    Ottesen, Marianne; Sørensen, Mette; Kehlet, Henrik

    2003-01-01

    OBJECTIVE: Retrospectively to describe the recommended convalescence according to patients who had undergone vaginal prolapse surgery in 1996-98, and prospectively to describe the need for and limiting factors for convalescence after vaginal prolapse surgery in 1999-2000 at a Danish University...... Hospital. METHODS: The retrospective study included a validated, postal, questionnaire and review of patient files. In the prospective study, we followed consecutive women after vaginal surgery in a fast-track setting using a multimodal rehabilitation model with well-defined recommendations...... exceeding 10 kg. Limiting factors were fatigue and pain. The 1-year subjective recurrence rate was 17%. CONCLUSION: Traditionally, recommended convalescence has been median 6 weeks after vaginal prolapse surgery. Convalescence has been shortened to 1-3 weeks with a multimodal rehabilitation model...

  6. Inguinal hernia repair: anaesthesia, pain and convalescence.

    Science.gov (United States)

    Callesen, Torben

    2003-08-01

    Elective surgical repair of an inguinal or femoral hernia is one of the most common surgical procedures. The treatment, however, presents several challenges regarding anaesthesia for the procedure, the postoperative analgesic therapy and convalescence, as well as planning of the procedure. Local, general, and regional anaesthesia are all used for hernia repair, but to different degrees, primarily depending on traditions and whether the institution has specific interest in hernia surgery. Thus, the use of local anaesthesia varies from a few percent in Sweden, 18% in Denmark and up to almost 100% in specialised institutions, dedicated to hernia surgery. The feasibility of local anaesthesia is high, as judged by the rate of conversion to general anaesthesia (thirds have moderate or severe pain during activity, while one third still have moderate or severe pain after one week, and approximately 10% after 4 weeks. Pain after laparoscopic surgery is less pronounced than after open surgery, while different open repair techniques do not exhibit significant differences. Postoperative pain is best treated with a combination of local analgesia and peripherally acting agents (paracetamol, NSAID or their combination), while opioids should be avoided due to side effects, primarily nausea and sedation. Moderate or severe pain one year postoperatively is seen in 5-12% of patients. There seem to be no difference between different surgical or anaesthetic techniques, but the following factors have been related to a higher rate of chronic pain: previous or subsequent hernia surgery on the same side, young age, pain before surgery, high pain scores in the immediate postoperative period, and postoperative complications and prolonged convalescence. Patients should be informed about the risk of chronic pain, particularly if the hernia is asymptomatic. The duration of convalescence after hernia repair varies considerably, primarily due to variation in recommendations. No documentation is

  7. Recovery and convalescence after laparoscopic surgery for appendicitis

    DEFF Research Database (Denmark)

    Kleif, Jakob; Vilandt, Jesper; Gögenur, Ismail

    2016-01-01

    BACKGROUND: Information about predictors for the duration of convalescence and the overall general wellbeing after laparoscopic surgery for suspected appendicitis is missing in the scientific literature. We aimed to describe and identify predictors for the duration of convalescence and the qualit...

  8. Inguinal hernia repair: anaesthesia, pain and convalescence

    DEFF Research Database (Denmark)

    Callesen, Torben

    2003-01-01

    Elective surgical repair of an inguinal or femoral hernia is one of the most common surgical procedures. The treatment, however, presents several challenges regarding anaesthesia for the procedure, the postoperative analgesic therapy and convalescence, as well as planning of the procedure. Local......, general, and regional anaesthesia are all used for hernia repair, but to different degrees, primarily depending on traditions and whether the institution has specific interest in hernia surgery. Thus, the use of local anaesthesia varies from a few percent in Sweden, 18% in Denmark and up to almost 100......% in specialised institutions, dedicated to hernia surgery. The feasibility of local anaesthesia is high, as judged by the rate of conversion to general anaesthesia (

  9. Cardiac magnetic resonance feature tracking in Kawasaki disease convalescence

    Directory of Open Access Journals (Sweden)

    Konstantinos Bratis

    2017-01-01

    Conclusion: In this CMR study in Kawasaki disease convalescent patients with preserved routine functional indices, we detected lower circumferential and longitudinal strain values compared to normal controls, irrespective of the coronary artery status.

  10. Determinants of a short convalescence after laparoscopic transabdominal preperitoneal inguinal hernia repair

    DEFF Research Database (Denmark)

    Tolver, Mette Astrup; Strandfelt, Pernille; Forsberg, Gert;

    2012-01-01

    Evidence-based recommendations for the expected duration of convalescence after laparoscopic groin hernia repair are not available, and objective reasons for prolonged convalescence are not clear. Our main aim was to establish the expected duration of convalescence using preoperative recommendati......Evidence-based recommendations for the expected duration of convalescence after laparoscopic groin hernia repair are not available, and objective reasons for prolonged convalescence are not clear. Our main aim was to establish the expected duration of convalescence using preoperative...... recommendations to the patient and to identify the limiting factors for early (postoperative) resumption of normal activities after laparoscopic transabdominal preperitoneal inguinal herniorraphy (TAPP)....

  11. Cardiac magnetic resonance feature tracking in Kawasaki disease convalescence.

    Science.gov (United States)

    Bratis, Konstantinos; Hachmann, Pauline; Child, Nicholas; Krasemann, Thomas; Hussain, Tarique; Mavrogeni, Sophie; Botnar, Rene; Razavi, Reza; Greil, Gerald

    2017-01-01

    The objective of this study was to determine whether left ventricular (LV) myocardial deformation indices can detect subclinical abnormalities in Kawasaki disease convalescence. We hypothesized that subclinical myocardial abnormalities due to inflammation represent an early manifestation of the disease that persists in convalescence. Myocardial inflammation has been described as a global finding in the acute phase of Kawasaki disease. Despite normal systolic function by routine functional measurements, reduced longitudinal strain and strain rate have been detected by echocardiography in the acute phase. Peak systolic LV myocardial longitudinal, radial, and circumferential strain and strain rate were examined in 29 Kawasaki disease convalescent patients (15 males; mean [standard deviation] age: 11 [6.6] years; median interval from disease onset: 5.8 [5.4] years) and 10 healthy volunteers (5 males; mean age: 14 [3.8] years) with the use of cardiac magnetic resonance (CMR) feature tracking. Routine indices of LV systolic function were normal in both groups. Comparisons were made between normal controls and (i) the entire Kawasaki disease group, (ii) Kawasaki disease subgroup divided by coronary artery involvement. Average longitudinal and circumferential strain at all levels was lower in patients compared to normal controls. In subgroup analysis, both Kawasaki disease patients with and without a history of coronary involvement had similar longitudinal and circumferential strain at all levels and lower when compared to controls. There were lower circumferential and longitudinal values in Kawasaki disease patients with persisting coronary artery lesions when compared to those with regressed ones. In this CMR study in Kawasaki disease convalescent patients with preserved routine functional indices, we detected lower circumferential and longitudinal strain values compared to normal controls, irrespective of the coronary artery status.

  12. Pain and convalescence following laparoscopic ventral hernia repair

    DEFF Research Database (Denmark)

    Eriksen, Jens Ravn

    satisfaction. This issue must have first priority in future ventral hernia repair research. It is now documented, that the simple application of fibrin glue instead of titanium tacks for mesh fixation in LVHR of defects ...) assess the intensity and impact of postoperative pain by detailed patient-reported description of pain and convalescence after LVHR (Study I), 2) evaluate the feasibility of fibrin sealant (FS) for mesh fixation in an experimental pig model (Study II), and 3) investigate FS vs. tacks for mesh fixation...

  13. Uveitis and Systemic Inflammatory Markers in Convalescent Phase of Ebola Virus Disease.

    Science.gov (United States)

    Chancellor, John R; Padmanabhan, Sriranjani P; Greenough, Thomas C; Sacra, Richard; Ellison, Richard T; Madoff, Lawrence C; Droms, Rebecca J; Hinkle, David M; Asdourian, George K; Finberg, Robert W; Stroher, Ute; Uyeki, Timothy M; Cerón, Olga M

    2016-02-01

    We report a case of probable Zaire Ebola virus-related ophthalmologic complications in a physician from the United States who contracted Ebola virus disease in Liberia. Uveitis, immune activation, and nonspecific increase in antibody titers developed during convalescence. This case highlights immune phenomena that could complicate management of Ebola virus disease-related uveitis during convalescence.

  14. Substantial variability in postoperative treatment, and convalescence recommendations following vaginal repair. A nationwide questionnaire study

    DEFF Research Database (Denmark)

    Ottesen, Marianne; Møller, Charlotte; Kehlet, H

    2001-01-01

    BACKGROUND: Postoperative care and convalescence recommendations following vaginal surgery are generally not evidence based. The aim of this study was to describe pre and postoperative treatment, and advice and restrictions for the convalescence period, given by Danish hospital-employed gynecolog...

  15. Use of convalescent plasma in Ebola virus infection.

    Science.gov (United States)

    Garraud, Olivier

    2017-02-01

    The recent Ebola virus epidemics which threatened three West African countries (Dec.2014-Apr.2016) has urged global collaborative health organizations and countries to set up measures to stop the infection and to treat patients, near half of them being at risk of death. Convalescent plasma-recovered from rescued West Africans-was considered a feasible therapeutic option. Efficacy was difficult to evaluate because of numerous unknowns (especially evolution of neutralizing antibodies), prior to the cessation of active transmission. This raises a large body of questions spanning epidemiological, virological, immunological but also ethical, sociological and anthropological aspects, alongside with public health concerns, in order to be better prepared to the next outbreak. This essay summarizes efforts made by a large number of groups worldwide, and attempts to address still unanswered questions on the benefit of specific versus non-specific plasma on altered-leaking-vascular endothelia in Ebola infection. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Pain and convalescence following laparoscopic ventral hernia repair

    DEFF Research Database (Denmark)

    Eriksen, Jens Ravn

    was 7%. No parameter predicted postoperative pain significantly. Significant correlations were found between pain, and general well-being (rS= -0.8, p satisfaction (rS= -0.67, p life score (rS= -0.63, p ... in LVHR in a randomised, double-blinded, clinical controlled study with acute postoperative pain as the primary outcome (Study III). In Study I - a prospective descriptive study - 35 patients were prospectively included and underwent LVHR. Scores of pain, quality of life, convalescence, fatigue......, and general well-being were obtained from each patient. Follow-up was six months. Average pain from postoperative day (POD) 0-2 and POD 0-6 measured on a 0-100 mm visual analogue scale (VAS) was 61 and 48, respectively. Pain scores reached preoperative values at POD 30. The incidence of severe chronic pain...

  17. Pain and convalescence following laparoscopic ventral hernia repair

    DEFF Research Database (Denmark)

    Eriksen, Jens Ravn

    2011-01-01

    was 7%. No parameter predicted postoperative pain significantly. Significant correlations were found between pain, and general well-being (rS= -0.8, p satisfaction (rS= -0.67, p life score (rS= -0.63, p ... in LVHR in a randomised, double-blinded, clinical controlled study with acute postoperative pain as the primary outcome (Study III). In Study I - a prospective descriptive study - 35 patients were prospectively included and underwent LVHR. Scores of pain, quality of life, convalescence, fatigue......, and general well-being were obtained from each patient. Follow-up was six months. Average pain from postoperative day (POD) 0-2 and POD 0-6 measured on a 0-100 mm visual analogue scale (VAS) was 61 and 48, respectively. Pain scores reached preoperative values at POD 30. The incidence of severe chronic pain...

  18. Prospective analysis of convalescence and early pain after uncomplicated laparoscopic fundoplication

    DEFF Research Database (Denmark)

    Bisgaard, T; Støckel, M; Klarskov, B

    2004-01-01

    fundoplication for gastro-oesophageal reflux disease. Patients were recommended to convalesce for 2 days after operation. Duration of convalescence, dysphagia, fatigue, nausea, vomiting and different pain components were registered daily during the first week and on days 10 and 30 after fundoplication. RESULTS...... or severe dysphagia during the study period. Fatigue scores were significantly increased for 6 days after surgery (P ... and dysphagia are significant problems after uncomplicated total laparoscopic fundoplication. The time taken off work and away from recreational activity exceeded the recommended 2 days of convalescence, justifying further efforts to optimize early clinical outcome after total laparoscopic fundoplication....

  19. A modified Delphi method toward multidisciplinary consensus on functional convalescence recommendations after abdominal surgery

    NARCIS (Netherlands)

    Vliet, D.C. van; Meij, E van der; Bouwsma, E.V.; Noordegraaf, A. Vonk; Heuvel, B. van den; Meijerink, W.J.H.J.; Baal, W.M. van; Huirne, J.A.; Anema, J.R.

    2016-01-01

    BACKGROUND: Evidence-based information on the resumption of daily activities following uncomplicated abdominal surgery is scarce and not yet standardized in medical guidelines. As a consequence, convalescence recommendations are generally not provided after surgery, leading to patients' insecurity,

  20. Ebola virus convalescent blood products: where we are now and where we may need to go.

    Science.gov (United States)

    Burnouf, Thierry; Seghatchian, Jerard

    2014-10-01

    The world is regularly exposed to emerging infections with the potential to burst into a pandemic. One possible way to treat patients, when no other treatment is yet developed,is passive immunization performed by transfusing blood, plasma or plasma immunoglobul infractions obtained from convalescent donors who have recovered from the disease and have developed protective antibodies. The most recent on-going epidemic is caused by the Ebola virus, a filovirus responsible for Ebola virus disease, a severe, often lethal, hemorrhagic fever. Recently, the use of convalescent blood products was proposed by the WHO as one early option for treating patients with Ebola virus disease. This publication provides an overview of the various convalescent blood products and technological options that could theoretically be considered when there is a need to rely on this therapeutic approach.In countries without access to advanced blood-processing technologies, the choice may initially be restricted to convalescent whole blood or plasma. In technologically advanced countries, additional options for convalescent blood products are available, including virally inactivated plasma and fractionated immunoglobulins. The preparation of minipool immunoglobulins is also a realistic option to consider.

  1. Convalescence after colonic resection with fast-track versus conventional care

    DEFF Research Database (Denmark)

    Hjort Jakobsen, D; Sonne, E; Basse, L;

    2004-01-01

    BACKGROUND: Multi-modal rehabilitation programmes may improve early postoperative body composition, pulmonary function, exercise capacity, and reduce hospital stay. So far, no data are available on convalescence after discharge. AIM: The objectives were to compare convalescence data (fatigue, sleep...... results in earlier resumption of normal activities with reduced fatigue and need for sleep postoperatively compared to conventional care, and without increased need for nursing care or visits to general practitioners. However, readmissions may occur more frequently....... vs. 8 days in the fast-track vs. conventional care group, respectively (p sleep were increased in the conventional care group when compared with the fast-track group (p

  2. Short convalescence after inguinal herniorrhaphy with standardised recommendations: duration and reasons for delayed return to work

    DEFF Research Database (Denmark)

    Callesen, T; Klarskov, B; Bech, K;

    1999-01-01

    inguinal herniorrhaphy under local anaesthesia. One day convalescence for light/moderate and three weeks for strenuous physical activity was recommended. MAIN OUTCOME MEASURE: Duration of absence from work or main recreational activity. RESULTS: Overall median absence (including the day of operation) was 6...

  3. Shelter-based convalescence for homeless adults in Amsterdam: a descriptive study

    NARCIS (Netherlands)

    van Laere, I.; de Wit, M.; Klazinga, N.

    2009-01-01

    ABSTRACT: BACKGROUND: Adequate support for homeless populations includes shelter and care to recuperate from illness and/or injury. This is a descriptive analysis of diagnoses and use of shelter-based convalescence in a cohort of homeless adults in Amsterdam. METHODS: Demographics of ill homeless

  4. Substantial variability in postoperative treatment, and convalescence recommendations following vaginal repair. A nationwide questionnaire study

    DEFF Research Database (Denmark)

    Ottesen, Marianne; Møller, Charlotte; Kehlet, H

    2001-01-01

    (range, 1-12), and the recommended time till recommencement of sexual intercourse was median 4 weeks (range, 0-12). The recommended convalescence was median 4-5 weeks for strenuous activities, and median 1-2 weeks for non-strenuous activities, with ranges from 0-24 weeks. The overall, great variance...

  5. Successful treatment of avian-origin influenza A (H7N9 infection using convalescent plasma

    Directory of Open Access Journals (Sweden)

    Xiao-Xin Wu

    2015-12-01

    Full Text Available In January 2015, there was an outbreak of avian-origin influenza A (H7N9 virus in Zhejiang Province, China. A 45-year-old man was admitted to the First Affiliated Hospital of Zhejiang University with a high fever that had lasted 7 days, chills, and a cough with yellow sputum. Laboratory testing confirmed infection with the H7N9 virus, likely obtained from contact with poultry at a local live poultry market. A large dense shadow was apparent in the patient's left lung at the time of admission. Treatment with oseltamivir (75 mg twice daily did not improve the patient's condition. The decision was made to try using convalescent plasma to treat the infection. Convalescent plasma was administered 3 days after the patient was admitted to the hospital and led to a marked improvement. To our knowledge, this is the first report of the successful use of convalescent plasma to treat a case of H7N9 infection in China. These results suggest that the combination of convalescent plasma and antiviral drugs may be effective for the treatment of avian-origin H7N9 infection.

  6. Successful treatment of avian-origin influenza A (H7N9) infection using convalescent plasma.

    Science.gov (United States)

    Wu, Xiao-Xin; Gao, Hai-Nv; Wu, Hai-Bo; Peng, Xiu-Ming; Ou, Hui-Lin; Li, Lan-Juan

    2015-12-01

    In January 2015, there was an outbreak of avian-origin influenza A (H7N9) virus in Zhejiang Province, China. A 45-year-old man was admitted to the First Affiliated Hospital of Zhejiang University with a high fever that had lasted 7 days, chills, and a cough with yellow sputum. Laboratory testing confirmed infection with the H7N9 virus, likely obtained from contact with poultry at a local live poultry market. A large dense shadow was apparent in the patient's left lung at the time of admission. Treatment with oseltamivir (75mg twice daily) did not improve the patient's condition. The decision was made to try using convalescent plasma to treat the infection. Convalescent plasma was administered 3 days after the patient was admitted to the hospital and led to a marked improvement. To our knowledge, this is the first report of the successful use of convalescent plasma to treat a case of H7N9 infection in China. These results suggest that the combination of convalescent plasma and antiviral drugs may be effective for the treatment of avian-origin H7N9 infection.

  7. Porin A-specific antibody avidity in patients who are convalescing from meningococcal B disease.

    NARCIS (Netherlands)

    Vermont, C.L.; Dijken, H.H. van; Groot, R. de; Dobbelsteen, G.P. van den

    2005-01-01

    Porin A (PorA), which determines the serosubtype of Neisseria meningitidis, is the main antigen of a candidate vaccine against serogroup B meningococci, which has been shown to induce high-avidity antibodies in children. We characterized the immune response of children after convalescing from

  8. Convalescence and hospital stay after colonic surgery with balanced analgesia, early oral feeding, and enforced mobilisation

    DEFF Research Database (Denmark)

    Møiniche, S; Bülow, Steffen; Hesselfeldt, Peter;

    1995-01-01

    OBJECTIVE: To evaluate the combined effects of pain relief by continuous epidural analgesia, early oral feeding and enforced mobilisation on convalescence and hospital stay after colonic resection. DESIGN: Uncontrolled pilot investigation. SETTING: University hospital, Denmark. SUBJECTS: 17....../daily. No patient had a nasogastric tube, and oral feeding with normal food and protein enriched solutions (1000 Kcal (4180 KJ/day) was instituted 24 hours postoperatively together with intensive mobilisation. RESULTS: Median visual analogue pain scores were zero at rest and minimal during coughing and mobilisation...... weight loss. CONCLUSION: These results suggest that a combined approach of optimal pain relief with balanced analgesia, enforced early mobilisation, and oral feeding, may reduce the length of convalescence and hospital stay after colonic operations....

  9. Analysis of the effectiveness of physical rehabilitation according spirographic indicators in community-acquired pneumonia during convalescence

    Directory of Open Access Journals (Sweden)

    Kalmykova Y.S.

    2014-05-01

    Full Text Available Purpose : to make a program of physical rehabilitation for convalescents after community-acquired pneumonia, promotes normalization of respiratory function. The objectives of the study was to evaluate the dynamics spirographic indicators during convalescence community-acquired pneumonia. Material: the study involved 28 women aged 19 to 24 years with a diagnosis of community-acquired pneumonia after convalescent. Results: the positive influence of physiotherapy based dance aerobics; morning hygienic gymnastics; therapeutic massage and physical therapy on indicators of lung volumes, ventilation and bronchial patency according spirographic research. Conclusion: in community-acquired pneumonia during the convalescence period recommended physical rehabilitation, which includes curative gymnastics based on dance aerobics, morning hygienic gymnastics, massage therapy, physiotherapy. It improves the functionality of the cardiorespiratory system, nonspecific immunity and overall physical performance level.

  10. Outcome assessment of stroke convalescence using multiple scales based on different dimensions

    Institute of Scientific and Technical Information of China (English)

    Yingrong Lao; Xinfeng Guo; Shilong Lai; Weixiong Liang; Zehuai Wen; Qi Wang; Peixin Huang; Yan Huang

    2006-01-01

    BACKGROUND: Convalescence is an important stage of stroke treatment. A lot of patients have somatic and mental disorders at various degrees. The primary standard can only reflect partial conditions of somatic disorder; in addition, multiple dimensions of patients at the phase of stroke convalescence are further observed by using a lot of standards, such as signs and symptoms of traditional Chinese medicine, daily activity and psychological status.OBJECTTVE: To analyze the outcome assessments of the cases of stroke convalescence measured with different criteria consisting of various dimensions by a cross-sectional investigation of the condition of stroke convalescent patients.DESIGN : Scale evaluation.SETTING: Departments of Clinical Epidemiology Exploratory Development and Neurology, the Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine; National Center for Training of Design, Measurement and Evaluation in Clinical Research,Guangzhou University of Traditional Chinese Medicine. PARTICIPANTS: A total of 194 stroke convalescent patients treated in the Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine from July 26, 2000 to February 28, 2001 were taken as subjects of the study. There were 126 males and 68 females aged from 40 to 89 years, and the illness course ranged from 14 to 181 days. All patients met diagnosis-treatment criteria of stroke (the second version)[DTCS(V2.0)] and various kinds of diagnostic points of cerebrovascular diseases; moreover, all patients provided confirmed consents.METHODS: They were assessed by assessment methods including the following assessment instruments:DTCS(V2.0), self-designed scale of traditional Chinese medicine (TCM) symptoms (28 symptoms and physical signs were scored as 0, 1, 2 marks from none to severity), modified Edinburgh-Scandinavia stroke scale (a total of 45 marks, 0 to 15 marks as mild defect, 16 to 30 as moderate defect, 31 to 45 as severe defect

  11. Convalescence and hospital stay after colonic surgery with balanced analgesia, early oral feeding, and enforced mobilisation

    DEFF Research Database (Denmark)

    Møiniche, S; Bülow, Steffen; Hesselfeldt, Peter;

    1995-01-01

    unselected patients (median age 69 years) undergoing colonic resection. INTERVENTIONS: Patients received combined epidural and general anaesthesia during operations and after operation were given continuous epidural bupivacaine 0.25%, 4 ml hour and morphine 0.2 mg hour, for 96 hours and oral paracetamol 4 g......OBJECTIVE: To evaluate the combined effects of pain relief by continuous epidural analgesia, early oral feeding and enforced mobilisation on convalescence and hospital stay after colonic resection. DESIGN: Uncontrolled pilot investigation. SETTING: University hospital, Denmark. SUBJECTS: 17...

  12. Alterations in cytokines and haematological parameters during the acute and convalescent phases of Plasmodium falciparum and Plasmodium vivax infections

    Directory of Open Access Journals (Sweden)

    Rodrigo Nunes Rodrigues-da-Silva

    2014-04-01

    Full Text Available Haematological and cytokine alterations in malaria are a broad and controversial subject in the literature. However, few studies have simultaneously evaluated various cytokines in a single patient group during the acute and convalescent phases of infection. The aim of this study was to sequentially characterise alterations in haematological patters and circulating plasma cytokine and chemokine levels in patients infected with Plasmodium vivax or Plasmodium falciparum from a Brazilian endemic area during the acute and convalescent phases of infection. During the acute phase, thrombocytopaenia, eosinopaenia, lymphopaenia and an increased number of band cells were observed in the majority of the patients. During the convalescent phase, the haematologic parameters returned to normal. During the acute phase, P. vivax and P. falciparum patients had significantly higher interleukin (IL-6, IL-8, IL-17, interferon-γ, tumour necrosis factor (TNF-α, macrophage inflammatory protein-1β and granulocyte-colony stimulating factor levels than controls and maintained high levels during the convalescent phase. IL-10 was detected at high concentrations during the acute phase, but returned to normal levels during the convalescent phase. Plasma IL-10 concentration was positively correlated with parasitaemia in P. vivax and P. falciparum-infected patients. The same was true for the TNF-α concentration in P. falciparum-infected patients. Finally, the haematological and cytokine profiles were similar between uncomplicated P. falciparum and P. vivax infections.

  13. 景观疗养护理对疗养员睡眠质量的影响%Effect of the Landscape Convalescence Convalescent Nursing on the Quality of Sleep

    Institute of Scientific and Technical Information of China (English)

    刘楠; 马佳

    2015-01-01

    Objective To explore the impact of landscape convalescence convalescent nursing on the quality of sleep.MethodsSelect the sanatorium in 100 cases, were randomly divided into control group and observation group with 50 cases in each group,the control group received routine nursing care,the observation group with landscape convalescence nursing.Results The quality score of two groups after nursing (including sleep sleep sleep,sustainability,mental status and social function) were higher than that before the treatment ,and sleeping quality in the observation groups were higher than those of the control group .Conclusion The implementation of landscape convalescence nursing on convalescent convalescent,sleep quality,nursing efect.%目的:探究景观疗养护理对疗养员睡眠质量的影响。方法选取疗养员100例,随机分为对照组和观察组各50例,对照组采用常规疗养护理,观察组采用景观疗养护理。结果护理后两组疗养员睡眠质量评分(包括睡眠效果、睡眠可持续性、精神状态和社会功能等)均高于护理前,且观察组疗养员睡眠质量评分较对照组高。结论对疗养员实施景观疗养护理,疗养员睡眠质量高,护理效果显著。

  14. Association of Leukoaraiosis With Convalescent Rehabilitation Outcome in Patients With Ischemic Stroke.

    Science.gov (United States)

    Senda, Joe; Ito, Keiichi; Kotake, Tomomitsu; Kanamori, Masahiko; Kishimoto, Hideo; Kadono, Izumi; Suzuki, Yoshiro; Katsuno, Masahisa; Nishida, Yoshihiro; Ishiguro, Naoki; Sobue, Gen

    2016-01-01

    We investigated the factors influencing inpatient convalescent rehabilitation outcomes in patients with ischemic stroke, particularly severity of leukoaraiosis on magnetic resonance imaging. Participants included 520 patients with ischemic stroke (317 men and 203 women; mean age, 72.8±8.4 years) who were transferred from acute care hospitals for inpatient convalescent rehabilitation. Ischemic stroke subtypes included lacunar infarction (n=41), atherothrombosis (n=223), artery-to-artery embolism (n=67), cardiogenic embolism (n=97), undetermined embolism (n=76), and uncategorized ischemic stroke (n=16). Leukoaraiosis was graded according to periventricular hyperintensity (PVH) and deep white matter hyperintensity on magnetic resonance imaging. Functional Independence Measure scores were assessed on admission and at discharge. Multiple regression analysis revealed that rehabilitation outcomes, measured as total Functional Independence Measure scores, were significantly associated with leukoaraiosis estimated by PVH grade. This association was observed after adjustment for factors such as severity, age, and poststroke history. In all patients, PVH grades were associated with Functional Independence Measure motor scores (PFunctional Independence Measure cognitive scores (Pstroke. Furthermore, the PVH grade was associated with motor function outcome, whereas the deep white matter hyperintensity grade correlated with cognitive function outcome, likely because the progression patterns and anatomic backgrounds of PVH and deep white matter hyperintensity differ according to ischemic stroke subtype. © 2015 American Heart Association, Inc.

  15. Effects of Standardized Rehabilitation on Quality of Life of Stroke Patients at Convalescence and Sequelae Stages

    Institute of Scientific and Technical Information of China (English)

    XU Dongyan; JIA Jie; WU Yi; LI Mingfen; XIE Hongyu; YU Kewei; GUO Zhenzhen

    2013-01-01

    Objective:To explore the effects of standardized rehabilitation on quality of life (QOL) of stroke patients at convalescence and sequelae stages.Method:A total of 251 stroke patients were randomly divided into a standardized rehabilitation group and a control group.The simplified Fugl-Meyer assessment(FMA) scale,the 36-item short-form health survey questionnaire (SF-36) and functional comprehensive assessment (FCA) were administered before as well as after 3,6and at 12 months a follow-up study respectively.Statistical analysis was conducted based on the evaluations at 4 testing time points.Result:There was no significant difference in FMA,SF-36 and FCA scores of the two groups before and after 3 months treatments,while FMA,SF-36 and FCA scores of the rehabilitation group were obviously higher than those of the control group either after 6 months treatments or of follow-up study.Moreover,after 6 months treatments FMA score was apparently higher than the score at the beginning and after 3 months treatments.The FMA,SF-36 and FAC scores during the follow-up visit decreased when compared with scores after 6 months treatments,but increased significantly when compared with the scores at the beginning and after 3months treatments.Conclusion:Standardized tertiary rehabilitation (STR) at convalescence and sequelae stages can significantly improve motor functions and QOL of stroke patients.

  16. Struggling for Independence: A Grounded Theory Study on Convalescence of ICU-survivors 12 Months Post ICU Discharge

    DEFF Research Database (Denmark)

    Ågård, Anne Sophie; Egerod, Ingrid; Tønnesen, Else Kirstine

    2012-01-01

    of getting well. Conclusion: The study offers new insight into post-ICU convalescence emphasising patients’ motivation for training to recover. The findings may contribute to defining the best supportive measures and timing of rehabilitation interventions in ICU and post ICU that may help ICU...

  17. Most frequent emotional states in convalescent patients of myocardial infarction and its relationship to cardiovascular health state

    Directory of Open Access Journals (Sweden)

    María C. García Martín

    2016-03-01

    Conclusions: There was a predominance of partially offset somatic state of health. High levels of anxiety and depression states were identified and it was found the existence of an important relation between anxiety-depression emotional states, and the somatic state of health relating to the cardiovascular system in patients convalescent from myocardial infarction.

  18. Convalescence and hospital stay after colonic surgery with balanced analgesia, early oral feeding, and enforced mobilisation

    DEFF Research Database (Denmark)

    Møiniche, S; Bülow, Steffen; Hesselfeldt, Peter

    1995-01-01

    OBJECTIVE: To evaluate the combined effects of pain relief by continuous epidural analgesia, early oral feeding and enforced mobilisation on convalescence and hospital stay after colonic resection. DESIGN: Uncontrolled pilot investigation. SETTING: University hospital, Denmark. SUBJECTS: 17....../daily. No patient had a nasogastric tube, and oral feeding with normal food and protein enriched solutions (1000 Kcal (4180 KJ/day) was instituted 24 hours postoperatively together with intensive mobilisation. RESULTS: Median visual analogue pain scores were zero at rest and minimal during coughing and mobilisation......, which allowed early mobilisation for up to 11 hours on the third postoperative day. Gastrointestinal function with defaecation had returned to normal in 12 patients within the first two postoperative days. Median hospital stay was five days with minimal increase in fatigue and without postoperative...

  19. The effect of balanced analgesia on early convalescence after major orthopaedic surgery

    DEFF Research Database (Denmark)

    Møiniche, S; Hjortsø, N C; Hansen, B L;

    1994-01-01

    Forty-two patients scheduled for total knee arthroplasty (n = 20) or hip arthroplasty (n = 22) were randomly allocated to receive either continuous epidural bupivacaine/morphine for 48 h postoperatively plus oral piroxicam, or general anaesthesia followed by a conventional intramuscular opioid...... and acetaminophen regimen. Patients undergoing knee- or hip arthroplasty treated with epidural analgesia had significantly lower pain scores during mobilization under the 48 h epidural infusion compared with patients receiving conventional treatment, while no important differences were observed after cessation...... of the epidural regimen. However, the achieved pain relief had no impact on postoperative convalescence parameters, such as ambulation, patient activity including need for nursing care, fatigue or hospital stay. Late postoperative pain, fatigue and conservative attitudes and routines in the postoperative care...

  20. Combined intra-articular glucocorticoid, bupivacaine and morphine reduces pain and convalescence after diagnostic knee arthroscopy

    DEFF Research Database (Denmark)

    Rasmussen, Sten; Lorentzen, Jan S; Larsen, Allan S

    2002-01-01

    We studied the effect of intra-articullar saline vs. bupivacaine + morphine or bupivacaine morphine + methylprednisolone after diagnostic knee arthroscopy. In a double-blind randomized study, 60 patients undergoing diagnostic knee arthroscopy without a therapeutic procedure were allocated to groups...... receiving intra-articular saline, intra-articular bupivacaine 150 mg + morphine 4 mg or the same dose of bupivacaine + morphine + intra-articular methylprednisolone 40 mg at the end of arthroscopy during general anesthesia. All patients were instructed to resume normal activities immediately after...... the procedure. Pain during movement and walking, leg muscle force and joint effusion, use of crutches and duration of sick leave were assessed. A combination of bupivacaine and morphine reduced pain, duration of immobilization and of convalescence. The addition of methylprednisolone further reduced pain, use...

  1. Misdiagnostic analysis of clinically diagnosed severe acute respiratory syndrome after following up 197 convalescent patients

    Institute of Scientific and Technical Information of China (English)

    LIU You-ning; TIAN Qing; HU Hong; XIE Li-xin; FAN Bao-xing; XU Hong-min; CHEN Wei-jun

    2005-01-01

    @@ The severe acute respiratory syndrome (SARS) is an emerging and highly contagious infection caused by a newly discovered strain of coronavirus.1 Since the clinical case definition of SARS is similar to other severe atypical pneumonias, specific laboratory tests that can accurately diagnose SARS-associated coronavirus (SARS-CoV) infection are important. However, published data are insufficient to investigate whether clinically diagnosed SARS patients may include some non-SARS pneumonia. Therefore, we aimed to determine clinical and laboratory features to differentiate SARS patients from non-SARS pneumonias that could reduce misdiagnosis of SARS. A retrospective analysis of clinical and laboratory characteristics after the initial onset of SARS, as well as its convalescent-phase, was examined from clinically diagnosed 197 SARS patients.

  2. The role of haloaerosolotherapy in immunorehabilitation of convalescents after community acquired pneumonia

    Directory of Open Access Journals (Sweden)

    Olha Lemko

    2015-02-01

    Full Text Available Aim: Investigation of the peculiarities of different haloaerosoltherapy regimes influence (treatment with different intensity of haloaerosol load upon non-specific defense and cellular immunity at convalescents after community acquired pneumonia. Objectives: patients with community acquired pneumonia in the early convalescence period (after completing antibiotic therapy, who received treatment in conditions of artificial rock salt aerosol medium (haloaerosoltherapy. Material and Methods. 42 patients with non-severe community acquired pneumonia were examined in the early convalescence period before and after the course of haloaerosoltherapy, which was prescribed after antibacterial therapy. Immunological studies included: evaluation of phagocytic activity of neutrophils (PhAN - the percentage of phagocytic neutrophils, phagocytic number (PhN - average number of latex particles absorbed by a neutrophil; metabolism of neutrophils in the test with nitroblue tetrasolium (NBT-test spontaneous and induced, which allowed to assess the functional reserve of neutrophils (FR; calculation of cytochemical coefficient (CCC for lysosomal cationic proteins (LCP and for myeloperoxidase (MPO of neutrophils; number of T- and B-lymphocytes and their subpopulations (CD3+ -, CD4+ -, CD8+ -, CD22+ - lymphocytes, calculation the number of 0- lymphocytes and the ratio of CD4+ /CD8+ lymphocytes. Laboratory examinations were also conducted in 21 practically healthy individuals (control group. Two regimes of haloaerosoltherapy were used in recovery treatment of patients with community acquired pneumonia: treating complex №1 (TC-1 with standard haloaerosol load and with increased haloaerosol load (TC-2. Results. After completion the antibiotic therapy at patients with community acquired pneumonia the moderate inhibition of phagocytic activity of neutrophils (47,6±0,58% to 55,5±1,14% in control group remained and was accompanied with a decrease in neutrophil bactericidal

  3. The Use of TKM-100802 and Convalescent Plasma in 2 Patients With Ebola Virus Disease in the United States

    Science.gov (United States)

    Kraft, Colleen S.; Hewlett, Angela L.; Koepsell, Scott; Winkler, Anne M.; Kratochvil, Christopher J.; Larson, LuAnn; Varkey, Jay B.; Mehta, Aneesh K.; Lyon, G. Marshall; Friedman-Moraco, Rachel J.; Marconi, Vincent C.; Hill, Charles E.; Sullivan, James N.; Johnson, Daniel W.; Lisco, Steven J.; Mulligan, Mark J.; Uyeki, Timothy M.; McElroy, Anita K.; Sealy, Tara; Campbell, Shelley; Spiropoulou, Christina; Ströher, Ute; Crozier, Ian; Sacra, Richard; Connor, Michael J.; Sueblinvong, Viranuj; Franch, Harold A.; Smith, Philip W.; Ribner, Bruce S.; Smith, Philip; Hewlett, Angela; Schwedhelm, Shelly; Boulter, Kate; Beam, Elizabeth; Gibbs, Shawn; Lowe, John; Kratochvil, Chris; Sullivan, James; Johnson, Dan; Lisco, Steve; Piquette, Craig; Bailey, Kristina; Auxier, Joseph; Boer, Brian; Hanson, Travis; Kaseman, Julia; Khan, M. Salman; Rhee, Ji Hyun; Wells, Adam; Florescu, Diana; Kalil, Andre; Rupp, Mark; Becker, Valerie; Boeckman, Bridget; Elder, Erica; Fitch, Abby; Flood, Elizabeth; Freml, Meagan; Frigge, Roman; Hanzlik, Kelly Ann; Jensen, Lois; Kraus, Nicole; Molacek, Drew; Nevins, Jerry; Parker, Alicia; Peters, Jeff; Rand, Cheryl; Roesler, Karen; Ryalls, Kendall; Shradar, Morgan; Strain, Amanda; Sunderman, Timothy; Sundermeier, Jennifer; Swanhorst, John; Vasa, Angela; Bellinghausen, Jean; Freihaut, Frank; Denny, Susan; Mainelli, Lauren; Pinkney, Dee; Ray, Deborah; Jevne, Jay; Knight, Kalen; McCroy, Derrick; Nadeau, Ralph; Nightser, Anna; Iwen, Pete; Sambol, Tony; Herrera, Vicki; Morgan, David; Trotter, Sarah; Kerby, Amy; Peters, Sue; Southern, Timothy; Murphy, Caitlin; Morris, Rosanna; Nuss, Sue; Franco, Theresa; Ogden, Connie; Lazure, Julie; Straub, Dawn; Koepsell, Scott; Hinrichs, Steve; Plumb, Troy; Florescu, Marius; Becker, John; Souchek, Jenna; Beck, Jon; Larson, Luann; Heires, Peggy; Gordon, Bruce; Paulsen, Gail; Wolford, Barb; Petersen, Jill; Marion, Nedra; Hayes, Kim; Tyner, Kate; Wilson, Taylor; Baltes, Paul; Nowatzke, Jenny; Nguyen, Jonathan; Turner, Paul; Boonstra, Barb; Jelden, Katelyn; Portrey, Randy; Stringfield, Doug; Scofield, Bryan; Svanda, Gary; Horihan, Jolene; Dahl, Chris; Bruno, Megan; Malm, Kelsey; Litz, Ron; Fehringer, Jessica; Paladino, Katie; Opp, Tammy; Bell, Sonia; Adams, Anne; Allen, Mary Beth; Bachman, Robert; Bornstein, William; Cantrell, Dee; Cosper, Pam; Feistritzer, Nancye; Fox, John; Gartland, Bryce; Goodman, Jen; Grant, Susan; Howard-Crow, Dallis; Horowitz, Ira; Pugh, David; Ritenour, Chad; Ash, Toni; Barnes, Christopher; Calhoun, Jason; Chapman, Lauren; Daye, Tracey; Durr, Haley; Evans, Shunasee; Gentry, Janice; Ginnane, Jan; Grant, Susan; Haynes, Chris; Hill, Carolyn; Hillis, Dustin; Johnson, Crystal; Loomis, Jessica; Mamora, Josia; Mitchell, Laura; Morgan, Jill; Osakwe, Nancy; Owen, Jacqueline; Piazza, Sarah; Shirley, Kristina; Siddens, Jodi; Silas, Carrie; Slabach, Jason; Tirador, Elaina; Todd, Donnette; Vanairsdale, Sharon; Brammer, Nicole; Buchanan, Juli; Burd, Eileen; Cardella, John; Eaves, Brenda; Evans, Crystal; Hostetler, Krista; Jenkins, Karen; Lindsey, Maureen; Magee, Jordan; Powers, Randall; Ritchie, James; Ryan, Emily; Bonds, Shannon; Emamifar, Amir; Kuban, Tish; Pack, Jan; Rogers, Susan; Golston, George; Kaufman, Sean; Olinger, Patricia; Olinger, Sean; Rengarajan, Kalpana; Thomaston, Scott; Beck, Emily; Desroches, Paula; Hall, Cynthia; Walker, Celeste; Bryant, Connie; Hackman, Betsy; Howard, Regina; Jones, Marolyn; Broughton, Jeff; Frisle, Brian; Jackson, Robert; Lewis, Jerry; Brown-Haithco, Robin; Gartin, Miranda Lynn; Geralds-Washington, Erica; James-Jones, Rhonda; Miller, Donald; Stark, Dan; McGee, Gentrice; Jones, Porcia; Scott-Harris, Linda; Cain, James; Davis, Roderick; Johnson, Tyrone; Pickett, Tyrone; Shaw, Anthony; Truesdale, Tenina; Blum, Jim; Hill, Laureen; Sevransky, Jon; Zivot, Joel; Walker, Seth; Klopman, Matthew; Matkins, Ricky; Meechan, Cathy; Meechan, Paul; Schwock, Kathy; Schuck, Jen; Stack, Kathy; Wolf, Francis; Bray, Bruce; Isakov, Alex; Shartar, Sam; Miles, Wade; Jamison, Aaro; Arevalo, John; Stallings, Gail; Christenbury, Janet; Dollard, Vince; De Gennaro, Melanie; Korschun, Holly; Seideman, Nancy; Ziegler, Tom; Griffith, Daniel P.; Dave, Nisha; Wall, Susan; Hall, Melida; McGhee, Dwania; Clarke, Tim; Vaught, Rachel; Peterson-Pileri, Katrina

    2015-01-01

    Background. The current West Africa Ebola virus disease (EVD) outbreak has resulted in multiple individuals being medically evacuated to other countries for clinical management. Methods. We report two patients who were transported from West Africa to the United States for treatment of EVD. Both patients received aggressive supportive care measures, as well as an investigational therapeutic (TKM-100802) and convalescent plasma. Results. While one patient experienced critical illness with multi-organ failure requiring mechanical ventilation and renal replacement therapy, both patients recovered without serious long-term sequelae to date. Conclusions. It is unclear what role the experimental drug and convalescent plasma had in the recovery of these patients. Prospective clinical trials are needed to delineate the role of investigational therapies in the care of patients with EVD. PMID:25904375

  4. Biofilm and planktonic pneumococci demonstrate disparate immunoreactivity to human convalescent sera

    Directory of Open Access Journals (Sweden)

    Shivshankar Pooja

    2011-11-01

    Full Text Available Abstract Background Streptococcus pneumoniae (the pneumococcus is the leading cause of otitis media, community-acquired pneumonia (CAP, sepsis, and meningitis. It is now evident that S. pneumoniae forms biofilms during nasopharyngeal colonization; the former which facilitates persistence, the latter, a prerequisite for subsequent development of invasive disease. Proteomic evaluation of S. pneumoniae suggests the antigen profile available for host-recognition is altered as a consequence of biofilm growth. This has potentially meaningful implications in regards to adaptive immunity and protection from disseminated disease. We therefore examined the antigen profile of biofilm and planktonic pneumococcal cell lysates, tested their reactivity with human convalescent sera and that generated against biofilm pneumococci, and examined whether immunization with biofilm pneumococci protected mice against infectious challenge. Results Biofilm pneumococci have dramatically altered protein profiles versus their planktonic counterparts. During invasive disease the humoral immune response is skewed towards the planktonic protein profile. Immunization with biofilm bacteria does not elicit a strong-cross-reactive humoral response against planktonic bacteria nor confer resistance against challenge with a virulent isolate from another serotype. We identified numerous proteins, including Pneumococcal serine-rich repeat protein (PsrP, which may serve as a protective antigens against both colonization and invasive disease. Conclusion Differential protein production by planktonic and biofilm pneumococci provides a potential explanation for why individuals remain susceptible to invasive disease despite previous colonization events. These findings also strongly suggest that differential protein production during colonization and disease be considered during the selection of antigens for any future protein vaccine.

  5. Immune Responses in Acute and Convalescent Patients with Mild, Moderate and Severe Disease during the 2009 Influenza Pandemic in Norway.

    Science.gov (United States)

    Mohn, Kristin G-I; Cox, Rebecca Jane; Tunheim, Gro; Berdal, Jan Erik; Hauge, Anna Germundsson; Jul-Larsen, Åsne; Peters, Bjoern; Oftung, Fredrik; Jonassen, Christine Monceyron; Mjaaland, Siri

    2015-01-01

    Increased understanding of immune responses influencing clinical severity during pandemic influenza infection is important for improved treatment and vaccine development. In this study we recruited 46 adult patients during the 2009 influenza pandemic and characterized humoral and cellular immune responses. Those included were either acute hospitalized or convalescent patients with different disease severities (mild, moderate or severe). In general, protective antibody responses increased with enhanced disease severity. In the acute patients, we found higher levels of TNF-α single-producing CD4+T-cells in the severely ill as compared to patients with moderate disease. Stimulation of peripheral blood mononuclear cells (PBMC) from a subset of acute patients with peptide T-cell epitopes showed significantly lower frequencies of influenza specific CD8+ compared with CD4+ IFN-γ T-cells in acute patients. Both T-cell subsets were predominantly directed against the envelope antigens (HA and NA). However, in the convalescent patients we found high levels of both CD4+ and CD8+ T-cells directed against conserved core antigens (NP, PA, PB, and M). The results indicate that the antigen targets recognized by the T-cell subsets may vary according to the phase of infection. The apparent low levels of cross-reactive CD8+ T-cells recognizing internal antigens in acute hospitalized patients suggest an important role for this T-cell subset in protective immunity against influenza.

  6. Convalescent pigs are protected completely against infection with a homologous Actinobacillus pleuropneumoniae strain but incompletely against a heterologous-serotype strain

    NARCIS (Netherlands)

    Cruijsen, A.L.M.; Leengoed, van L.A.M.G.; Ham-Hoffjes, M.; Verheijden, J.H.M.

    1995-01-01

    To study whether Actinobacillus pleuropneumoniae induces a species- specific immunity, we infected pigs in the left lung with serotype 3 or 9 and after 3 weeks we infected their right lungs with serotype 9. Convalescent pigs were protected against homologous strain reinfection, but after

  7. Application of empowerment theory in chronic disease management of convalescents%授权赋能在疗养员慢性病管理中的应用

    Institute of Scientific and Technical Information of China (English)

    吴月美; 费秀霞; 鲍艳英

    2012-01-01

    Objective To explore the methods and effects of empowerment theory in chronic disease management of convalescents. Methods The measures included creating a relaxed and harmonious environment to encourage emotion expression of convalescents, identifying health problems of convalescents through the health assessment, and setting up feasible recovery targets. Nurses provided professional guidance of health education on convalescents by using professional knowledge and skills, and encouraged them to participate in disease self - care actively, develop nursing plans and organize the implementation, and make the final evaluation feedback. Results The chronic disease knowledge and disease self - care skills of convalescents were improved. Except for blood pressure and high -density lipoprotein,the other health monitoring indicators were statistically significant difference (P<0.01 or P <0.05). Conclusion By using the empowerment in chronic disease management of convalescents, the convalescents can master the health - related knowledge and skills, and have the ability and confidence to control their own health. Empowerment plays a good role in promoting self - management of chronic diseases.%目的 探讨授权赋能在疗养员慢性病管理中的应用方法与效果.方法 营造轻松和谐的疗养环境,鼓励疗养员表达情感;健康评估,帮助疗养员明确健康问题;设立可行的疗养目标.护士利用专业知识和技能对疗养员进行健康教育并提供专业指导.鼓励他们主动参与疾病自我护理,制订疗养计划并组织实施,最后评价反馈.结果 疗养员提高了慢性病相关知识及疾病自我护理技能,除血压及高密度脂蛋白指标外,其余健康监测指标差异均具有统计学意义(P<0.01或P<0.05).结论 以授权赋能理论为指导,对疗养员的慢性病进行管理,使疗养员掌握了相关保健知识和技能,获得了控制自身健康的能力和自信,对慢性病自我管理具有良好的促进作用.

  8. Investigation of Hobbies and Interests of Convalescent Patients in Special Service Recuperation%特勤疗养员兴趣爱好调查

    Institute of Scientific and Technical Information of China (English)

    侯建敏; 张法胜; 李嵘; 杨玲

    2016-01-01

    Objective To investigate personal hobbies and interests of convalescent patients in special service recuperation through questionnaire and to formulate recuperation protocols .Methods A questionnaire list-ing 11 related hobbies and interests was used to survey personal hobbies and interests of convalescent patients in special service recuperation.Results According to personal hobbies and interests of convalescent patients in spe -cial service recuperation, individualized recuperation service was arranged , which improved their satisfaction rate and quality of recuperation.Conclusion Using questionnaire of hobbies and interests in special service recupera -tion not only meets individual needs of convalescent patients but also improves satisfaction rate and quality of recu -peration service.%目的:通过兴趣爱好调查表了解特勤疗养员的个人喜好,科学制定相应的疗养计划。方法通过11项相关爱好项目的调查,了解掌握特勤疗养员的个人喜好。结果针对特勤疗养员的喜好,组织个性化的疗养活动安排,提高特勤疗养员满意率,疗养质量也有较大提高。结论兴趣爱好调查表在特勤疗养中的应用,不仅满足特勤疗养员的个人需求,而且能提高疗养服务满意率及疗养服务保障质量。

  9. Nutritional Improvement Correlates with Recovery of Activities of Daily Living among Malnourished Elderly Stroke Patients in the Convalescent Stage: A Cross-Sectional Study.

    Science.gov (United States)

    Nishioka, Shinta; Wakabayashi, Hidetaka; Nishioka, Emi; Yoshida, Tomomi; Mori, Natsumi; Watanabe, Riko

    2016-05-01

    Whether nutritional improvement correlates with functional recovery in convalescent stroke patients is unclear. This study was conducted to examine the relationship between nutritional improvement and recovery of activities of daily living among malnourished elderly stroke patients in the convalescent stage. This study used a cross-sectional study design. One hundred seventy-eight malnourished stroke patients aged 65 years and older from convalescent rehabilitation wards in Japan between April 2012 and December 2014 were included in the analyses. The participants were classified into three groups according to the Mini Nutritional Assessment Short-Form (MNA-SF) score at discharge (0 to 7 as no improvement, 8 to 11 as lesser improvement, and 12 to 14 as greater improvement). The primary outcome was functional independence measure (FIM) efficiency (FIM gain/length of hospital stay). The secondary outcomes were FIM gain and discharge outcome. One-way analysis of variance, χ(2) test, and Kruskal-Wallis test were performed for univariate analysis. Linear regression analysis was used to adjust for covariates such as age, sex, length of hospital stay, FIM (motor and cognitive) on admission, and lower-order items of MNA-SF. Binomial logistic analysis for discharge outcome (home/others) was performed to adjust for covariates such as age, sex, and FIM. Study participants included 85 men and 93 women with a mean age of 77 years. Based on MNA-SF, 16 were classified as no improvement, 113 as lesser improvement, and 49 as greater improvement. The median FIM efficiency and length of hospital stay were 0.27 points/day and 151.5 days, respectively. The greater improvement group had significantly higher FIM efficiency than the other groups (Pnutritional improvement such as maintenance of body weight is associated with the efficient recovery of activities of daily living among malnourished elderly convalescent stroke patients. Copyright © 2016 Academy of Nutrition and Dietetics

  10. Platelet degranulation and monocyte-platelet complex formation are increased in the acute and convalescent phases after ischaemic stroke or transient ischaemic attack.

    LENUS (Irish Health Repository)

    McCabe, Dominick J H

    2004-06-01

    Flow cytometric studies suggest that platelets are activated in ischaemic stroke or transient ischaemic attack (TIA). However, few studies have measured circulating leucocyte-platelet complexes in this patient population. Whole blood flow cytometry was used to quantify the expression of CD62P-, CD63-, and PAC1-binding, and the percentages of leucocyte-platelet complexes in acute (1-27 d, n = 79) and convalescent (79-725 d, n = 70) ischaemic cerebrovascular disease (CVD) patients compared with controls without CVD (n = 27). We performed a full blood count, and measured plasma levels of soluble P-selectin, soluble E-selectin, and von Willebrand factor antigen (VWF:Ag) as additional markers of platelet and\\/or endothelial cell activation. The median percentage CD62P expression and the median percentage monocyte-platelet complexes were higher in both acute and convalescent CVD patients than controls (P <\\/= 0.02). The mean white cell count and mean VWF:Ag levels were significantly elevated in the acute and convalescent phases after ischaemic stroke or TIA (P <\\/= 0.02). Otherwise, there was no significant increase in any other marker of platelet or endothelial activation in CVD patients. There was a positive correlation between the percentage expression of CD62P and the percentages of both neutrophil-platelet and monocyte-platelet complexes in the acute phase, and the percentages of all leucocyte-platelet complexes in the convalescent phase after ischaemic CVD. This study provides evidence for ongoing excessive platelet and\\/or endothelial activation in ischaemic CVD patients despite treatment with antithrombotic therapy.

  11. Randomized controlled comparative study on effect of training to improve lower limb motor paralysis in convalescent patients with post-stroke hemiplegia

    OpenAIRE

    Kawakami, Kenji; Miyasaka, Hiroyuki; Nonoyama, Sayaka; HAYASHI, Kazuya; Tonogai, Yusuke; Tanino, Genichi; Wada, Yosuke; Narukawa, Akihisa; Okuyama, Yuko; Tomita, Yutaka; ,; Sonoda, Shigeru

    2015-01-01

    [Purpose] The motor paralysis-improving effect on the hemiplegic lower limb was compared among mirror therapy, integrated volitional-control electrical stimulation, therapeutic electrical stimulation, repetitive facilitative exercises, and the standard training method in post-stroke hemiplegia patients. [Subjects and Methods] Eighty one stroke patients admitted to a convalescent rehabilitation ward were randomly allocated to the above 5 treatment groups. Each patient performed functional trai...

  12. Comprehensive analysis of antibody recognition in convalescent humans from highly pathogenic avian influenza H5N1 infection.

    Science.gov (United States)

    Zuo, Teng; Sun, Jianfeng; Wang, Guiqin; Jiang, Liwei; Zuo, Yanan; Li, Danyang; Shi, Xuanling; Liu, Xi; Fan, Shilong; Ren, Huanhuan; Hu, Hongxing; Sun, Lina; Zhou, Boping; Liang, Mifang; Zhou, Paul; Wang, Xinquan; Zhang, Linqi

    2015-12-04

    Understanding the mechanism of protective antibody recognition against highly pathogenic avian influenza A virus H5N1 in humans is critical for the development of effective therapies and vaccines. Here we report the crystal structure of three H5-specific human monoclonal antibodies bound to the globular head of hemagglutinin (HA) with distinct epitope specificities, neutralization potencies and breadth. A structural and functional analysis of these epitopes combined with those reported elsewhere identifies four major vulnerable sites on the globular head of H5N1 HA. Chimeric and vulnerable site-specific mutant pseudoviruses are generated to delineate broad neutralization specificities of convalescent sera from two individuals who recovered from the infection with H5N1 virus. Our results show that the four vulnerable sites on the globular head rather than the stem region are the major neutralizing targets, suggesting that during natural H5N1 infection neutralizing antibodies against the globular head work in concert to provide protective antibody-mediated immunity.

  13. OBSERVATION ON THE THERAPEUTIC EFFECT OF ACU-MOXIBUSTION OF DIFFERENT ACUPOINT GROUPS IN STROKE PATIENTS IN CONVALESCENCE STAGE

    Institute of Scientific and Technical Information of China (English)

    DENG Bai-ying; ZHOU En-hua; SU Sheng-yong; LI Yang-fan

    2005-01-01

    Objective: To observe and compare clinical therapeutic effect of acupuncture and moxibustion at different acupoint groups in stroke patients in the convalescence stage. Methods: Sixty stroke patients were evenly randomized into yin-meridian group and yang-meridian group. On the basis of the treatment with Chinese and Western medicines, these stroke patients of two groups were treated mainly by acupuncture of acupoints of yin meridians such as Jiquan (极泉HT 1), Tongli (通里HT 5), Neiguan (内关PC 6), etc., and those of yang meridians as Jianyu (肩NFDA1 LI 15), Quchi (曲池 LI 11), Shousanli (手三里LI 10), etc. Respectively. After 30 days' treatment, the therapeutic effect was evaluated by using American National Institutes of Health Stroke Scale (NIHSS), Hamilton Depression (HAMD) Rating Scale, Mini-Mental State Examination (MMSE), Barthel Index and Activities of Daily Living (ADL) Scale. Results: Following treatment, the therapeutic effect of yin-meridian group was significantly superior to that of yang-meridian group in improving stroke patients' neurological deficits [Level B: 50% (15/30) vs 20% (6/30)] and ADL [Level Ⅱ: 40% (12/30) vs 13.3% (4/30)]. No significant difference was found between two groups in the neurological impairment domains. Conclusion: Acu-moxibustion of acupoints of yin meridians is superior to acupoints of yang meridians in improving stroke patients' neurological impairment and daily living activities.

  14. Organizing the Donation of Convalescent Plasma for a Therapeutic Clinical Trial on Ebola Virus Disease: The Experience in Guinea

    Science.gov (United States)

    Delamou, Alexandre; Haba, Nyankoye Yves; Mari-Saez, Almudena; Gallian, Pierre; Ronse, Maya; Jacobs, Jan; Camara, Bienvenu Salim; Kadio, Kadio Jean-Jacques Olivier; Guemou, Achille; Kolie, Jean Pe; De Crop, Maaike; Chavarin, Patricia; Jacquot, Chantal; Lazaygues, Catherine; De Weggheleire, Anja; Lynen, Lutgarde; van Griensven, Johan

    2016-01-01

    Although convalescent plasma (CP) transfusion was prioritized among potential Ebola treatments by the World Health Organization, there were concerns on the feasibility of its implementation. We report on the successful organization of donor mobilization and plasma collection as part of the Ebola-Tx clinical trial from November 2014 to July 2015 in Conakry, Guinea. Project implementation registers, tools and reports, mission reports, and minutes of research team meetings were used to reconstruct the sequence of events on how donor mobilization was organized, plasmapheresis was set up, and how effective this approach was in collecting CP. An initial needs assessment of the Guinean National Blood Transfusion Center resulted in targeted training of staff on site, resulting in autonomy and independent production of CP within 3 months. The Conakry Ebola Survivors Association played a direct role in donor mobilization and organization of CP donations. A total of 98 Ebola survivors were screened for plasma donation, of which 84 were found eligible for plasmapheresis. Of these, 26 (30.9%) were excluded. The remaining 58 donors made a total of 90 donations, corresponding to 50.9 L of CP. This sufficed to treat the 99 eligible patients enrolled in the trial. Within a poor resource emergency context, transfusion capacity could be rapidly improved through the strengthening of local capacities and gradual transfer of skills coupled with active involvement of Ebola survivors. However, large-scale plasma collection or multisite studies may require further adaptations of both strategy and logistics. The Ebola-Tx trial was funded by the European Union and others. PMID:27430546

  15. Generation of a convalescent model of virulent Francisella tularensis infection for assessment of host requirements for survival of tularemia.

    Directory of Open Access Journals (Sweden)

    Deborah D Crane

    Full Text Available Francisella tularensis is a facultative intracellular bacterium and the causative agent of tularemia. Development of novel vaccines and therapeutics for tularemia has been hampered by the lack of understanding of which immune components are required to survive infection. Defining these requirements for protection against virulent F. tularensis, such as strain SchuS4, has been difficult since experimentally infected animals typically die within 5 days after exposure to as few as 10 bacteria. Such a short mean time to death typically precludes development, and therefore assessment, of immune responses directed against virulent F. tularensis. To enable identification of the components of the immune system that are required for survival of virulent F. tularensis, we developed a convalescent model of tularemia in C57Bl/6 mice using low dose antibiotic therapy in which the host immune response is ultimately responsible for clearance of the bacterium. Using this model we demonstrate αβTCR(+ cells, γδTCR(+ cells, and B cells are necessary to survive primary SchuS4 infection. Analysis of mice deficient in specific soluble mediators shows that IL-12p40 and IL-12p35 are essential for survival of SchuS4 infection. We also show that IFN-γ is required for survival of SchuS4 infection since mice lacking IFN-γR succumb to disease during the course of antibiotic therapy. Finally, we found that both CD4(+ and CD8(+ cells are the primary producers of IFN-γand that γδTCR(+ cells and NK cells make a minimal contribution toward production of this cytokine throughout infection. Together these data provide a novel model that identifies key cells and cytokines required for survival or exacerbation of infection with virulent F. tularensis and provides evidence that this model will be a useful tool for better understanding the dynamics of tularemia infection.

  16. Organizing the Donation of Convalescent Plasma for a Therapeutic Clinical Trial on Ebola Virus Disease: The Experience in Guinea.

    Science.gov (United States)

    Delamou, Alexandre; Haba, Nyankoye Yves; Mari-Saez, Almudena; Gallian, Pierre; Ronse, Maya; Jacobs, Jan; Camara, Bienvenu Salim; Kadio, Kadio Jean-Jacques Olivier; Guemou, Achille; Kolie, Jean Pe; Crop, Maaike De; Chavarin, Patricia; Jacquot, Chantal; Lazaygues, Catherine; Weggheleire, Anja De; Lynen, Lutgarde; van Griensven, Johan

    2016-09-01

    Although convalescent plasma (CP) transfusion was prioritized among potential Ebola treatments by the World Health Organization, there were concerns on the feasibility of its implementation. We report on the successful organization of donor mobilization and plasma collection as part of the Ebola-Tx clinical trial from November 2014 to July 2015 in Conakry, Guinea. Project implementation registers, tools and reports, mission reports, and minutes of research team meetings were used to reconstruct the sequence of events on how donor mobilization was organized, plasmapheresis was set up, and how effective this approach was in collecting CP. An initial needs assessment of the Guinean National Blood Transfusion Center resulted in targeted training of staff on site, resulting in autonomy and independent production of CP within 3 months. The Conakry Ebola Survivors Association played a direct role in donor mobilization and organization of CP donations. A total of 98 Ebola survivors were screened for plasma donation, of which 84 were found eligible for plasmapheresis. Of these, 26 (30.9%) were excluded. The remaining 58 donors made a total of 90 donations, corresponding to 50.9 L of CP. This sufficed to treat the 99 eligible patients enrolled in the trial. Within a poor resource emergency context, transfusion capacity could be rapidly improved through the strengthening of local capacities and gradual transfer of skills coupled with active involvement of Ebola survivors. However, large-scale plasma collection or multisite studies may require further adaptations of both strategy and logistics. The Ebola-Tx trial was funded by the European Union and others.

  17. Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity

    Directory of Open Access Journals (Sweden)

    Kang Tang

    2017-01-01

    Full Text Available Haemorrhagic fever with renal syndrome (HFRS is characterised by an uncontrolled immune response that causes vascular leakage. Adiponectin (APN is an adipocytokine involved in prorevascularisation and immunomodulation. To investigate the possible effects of APN in the pathogenesis of HFRS, total and high molecular weight (HMW APN levels in the plasma of patients with HFRS were quantified using enzyme-linked immunosorbent assay (ELISA. Compared with those in healthy controls, the plasma total and HMW APN levels in patients were elevated to different degrees from the fever onset and remained high at the convalescent phase. Consistent with these results, western blot analysis additionally showed that low molecular weight (LMW, middle molecular weight (MMW, and HMW APN levels were all elevated and contributed to the elevation of the total APN level. Importantly, sustained high levels of total and HMW APN at the convalescent phase were significantly higher in patients with critical disease than those in patients with mild or moderate disease. Moreover, total and HMW APN levels negatively correlated with white blood cell count and positively correlated with platelet count and serum albumin level. These results may provide insights into understanding the roles of total and HMW APN in the pathogenesis of HFRS.

  18. Cells and mediators of inflammation (C-reactive protein, nitric oxide, platelets and neutrophils) in the acute and convalescent phases of uncomplicated Plasmodium vivax and Plasmodium falciparum infection.

    Science.gov (United States)

    Lima-Junior, Josué da Costa; Rodrigues-da-Silva, Rodrigo Nunes; Pereira, Virgínia Araújo; Storer, Fábio Luiz; Perce-da-Silva, Daiana de Souza; Fabrino, Daniela Leite; Santos, Fátima; Banic, Dalma Maria; Oliveira-Ferreira, Joseli de

    2012-12-01

    The haematological changes and release of soluble mediators, particularly C-reactive protein (CRP) and nitric oxide (NO), during uncomplicated malaria have not been well studied, especially in Brazilian areas in which the disease is endemic. Therefore, the present study examined these factors in acute (day 0) and convalescent phase (day 15) patients infected with Plasmodium falciparum and Plasmodium vivax malaria in the Brazilian Amazon. Haematologic parameters were measured using automated cell counting, CRP levels were measured with ELISA and NO plasma levels were measured by the Griess reaction. Our data indicate that individuals with uncomplicated P. vivax and P. falciparum infection presented similar inflammatory profiles with respect to white blood cells, with high band cell production and a considerable degree of thrombocytopaenia during the acute phase of infection. Higher CRP levels were detected in acute P. vivax infection than in acute P. falciparum infection, while higher NO was detected in patients with acute and convalescent P. falciparum infections. Although changes in these mediators cannot predict malaria infection, the haematological aspects associated with malaria infection, especially the roles of platelets and band cells, need to be investigated further.

  19. Characteristics of seroconversion and implications for diagnosis of post-treatment Lyme disease syndrome: acute and convalescent serology among a prospective cohort of early Lyme disease patients.

    Science.gov (United States)

    Rebman, Alison W; Crowder, Lauren A; Kirkpatrick, Allison; Aucott, John N

    2015-03-01

    Two-tier serology is often used to confirm a diagnosis of Lyme disease. One hundred and four patients with physician diagnosed erythema migrans rashes had blood samples taken before and after 3 weeks of doxycycline treatment for early Lyme disease. Acute and convalescent serologies for Borrelia burgdorferi were interpreted according to the 2-tier antibody testing criteria proposed by the Centers for Disease Control and Prevention. Serostatus was compared across several clinical and demographic variables both pre- and post-treatment. Forty-one patients (39.4%) were seronegative both before and after treatment. The majority of seropositive individuals on both acute and convalescent serology had a positive IgM western blot and a negative IgG western blot. IgG seroconversion on western blot was infrequent. Among the baseline variables included in the analysis, disseminated lesions (p Lyme disease. Furthermore, these findings underline the difficulty for rheumatologists in identifying a prior exposure to Lyme disease in caring for patients with medically unexplained symptoms or fibromyalgia-like syndromes.

  20. 军队疗养院实施舒适护理的 SWOT 分析%SWOT Analysis of Carrying out Comfortable Nursing in Military Convalescent Hospitals

    Institute of Scientific and Technical Information of China (English)

    李鹭; 谭春红; 李丽; 王俊

    2016-01-01

    运用 SWOT 分析法,对军队疗养院实施舒适护理的内部环境、外部条件进行系统的分析。内部环境的优势是符合自身的发展规律,有领导的高度重视、完备的制度规范、优秀的护士长队伍,劣势是软硬件环境的制约、年轻护士的不稳定性、新学科新业务的不适应;外部条件的机遇是顺应军队改革发展的方向、满足养生保健的社会需求、有成功的经验可借鉴,威胁是疗养员期望值不断提高、社会人才的趋利性流动、缺乏客观量化的评判标准。阐述必须通过定指南抓落实,充分顺应时代需求而发展;搭平台聚人心,积极营造干事创业的环境;改理念求创新,持续提升疗养护理的品质;重人文做精细,不断满足疗养保障的需求等措施;促进舒适护理在军队疗养院的落实,进而提升疗养护理的品质。%This paper, through SWOT analytic method, makes a systematic analysis of the internal and ex -ternal conditions in carrying out comfortable nursing in military convalescent hospitals .The advantages in the inter-nal environment include consistency with regular law of self -development of convalescent hospitals , leaders’suffi-cient attention, complete rules and regulations and a team of outstanding nurses , while the disadvantages are re -stricted software and hardware, instability of young nurses, inadaptability to new science and profession .As for the external conditions, there are favorable opportunities, including consistency with the trend of the military reform and development, meeting the social demand for healthcare , and successful experiences for reference , while the unfavorable conditions are increasingly high expectations from the recuperating personnel , tendency flow of social talents, and lack of quantized objective judging criteria .This paper also suggests that comfortable nursing work be put into effect and the quality of nursing work be

  1. The Use of Ebola Convalescent Plasma to Treat Ebola Virus Disease in Resource-Constrained Settings: A Perspective From the Field

    Science.gov (United States)

    van Griensven, Johan; De Weiggheleire, Anja; Delamou, Alexandre; Smith, Peter G.; Edwards, Tansy; Vandekerckhove, Philippe; Bah, Elhadj Ibrahima; Colebunders, Robert; Herve, Isola; Lazaygues, Catherine; Haba, Nyankoye; Lynen, Lutgarde

    2016-01-01

    The clinical evaluation of convalescent plasma (CP) for the treatment of Ebola virus disease (EVD) in the current outbreak, predominantly affecting Guinea, Sierra Leone, and Liberia, was prioritized by the World Health Organization in September 2014. In each of these countries, nonrandomized comparative clinical trials were initiated. The Ebola-Tx trial in Conakry, Guinea, enrolled 102 patients by 7 July 2015; no severe adverse reactions were noted. The Ebola-CP trial in Sierra Leone and the EVD001 trial in Liberia have included few patients. Although no efficacy data are available yet, current field experience supports the safety, acceptability, and feasibility of CP as EVD treatment. Longer-term follow-up as well as data from nontrial settings and evidence on the scalability of the intervention are required. CP sourced from within the outbreak is the most readily available source of anti-EVD antibodies. Until the advent of effective antivirals or monoclonal antibodies, CP merits further evaluation. PMID:26261205

  2. Analysis on Korean Tourist Market of Convalescent Care%韩国疗养保健客源市场分析

    Institute of Scientific and Technical Information of China (English)

    曹银华

    2012-01-01

    疗养保健客源市场是发展涉外疗养保健服务贸易的关键所在.早在2000年韩国已经步入老龄化社会.且老龄化趋势持续进一步加剧。利用韩国统计厅公布的资料,从韩国的人口结构、医疗保健、社会环境、社会福利分析八手,深入研究韩国疗养保健客源市场,发现韩国特定的人口结构状况及医疗保健等方面存在诸多问题.可为我国开发韩国老龄人口疗养保健市场提供可能性和必要性。%Tourisl market of convaleseent care is the key to develop foreign service trade in eonvalescent care. In 2000, Korea has entered the aging society, and the aging trend is further developing. Through the released data in Korean National Statistical Office, from the analysis on Korean population, health care, social environment and social welfare, this article studies the Korean tourist market of convalescent care deeply, and finds that there are many problems on Korean specific population structure and health care, which objectively makes it sure that there is a strong demand for health services, and it provides the possibility and necessity that China will develop the tourist market of convalescent care for Korean aging population.

  3. Detection of Foot-and-mouth Disease Virus RNA and Capsid Protein in Lymphoid Tissues of Convalescent Pigs Does Not Indicate Existence of a Carrier State.

    Science.gov (United States)

    Stenfeldt, C; Pacheco, J M; Smoliga, G R; Bishop, E; Pauszek, S J; Hartwig, E J; Rodriguez, L L; Arzt, J

    2016-04-01

    A systematic study was performed to investigate the potential of pigs to establish and maintain persistent foot-and-mouth disease virus (FMDV) infection. Infectious virus could not be recovered from sera, oral, nasal or oropharyngeal fluids obtained after resolution of clinical infection with any of five FMDV strains within serotypes A, O and Asia-1. Furthermore, there was no isolation of live virus from tissue samples harvested at 28-100 days post-infection from convalescent pigs recovered from clinical or subclinical FMD. Despite lack of detection of infectious FMDV, there was a high prevalence of FMDV RNA detection in lymph nodes draining lesion sites harvested at 35 days post-infection, with the most frequent detection recorded in popliteal lymph nodes (positive detection in 88% of samples obtained from non-vaccinated pigs). Likewise, at 35 dpi, FMDV capsid antigen was localized within follicles of draining lymph nodes, but without concurrent detection of FMDV non-structural protein. There was a marked decline in the detection of FMDV RNA and antigen in tissue samples by 60 dpi, and no antigen or viral RNA could be detected in samples obtained at 100 dpi. The data presented herein provide the most extensive investigation of FMDV persistence in pigs. The overall conclusion is that domestic pigs are unlikely to be competent long-term carriers of infectious FMDV; however, transient persistence of FMDV protein and RNA in lymphoid tissues is common following clinical or subclinical infection.

  4. Impact of the number of painful stimuli on life satisfaction among Korean industrial accident workers completing convalescence: dual mediating effects of self-esteem and sleeping time.

    Science.gov (United States)

    Choi, Wan-Suk; Kim, Bo-Kyung; Kim, Ki-Do; Moon, Ok-Kon; Yeum, Dong-Moon

    2016-10-08

    This study examined the impact of the number of painful stimuli on life satisfaction among workers who experienced an industrial accident and investigated how self-esteem and sleeping time affected life satisfaction. The Korea Workers' Compensation & Welfare Service conducted the first nationwide panel survey on occupational health and safety insurance in 2013-2014 through a stratified systematic sampling on 2,000 industrial accident workers who completed convalescence. Based on the dataset, our study analyzed 1,832 workers experiencing an industrial accident after excluding 168 disease patients. For the research model analysis, a four-stage hierarchical regression analysis technique was applied using the SPSS regression analysis Macro program of PROCESS Procedure. To test mediated indirect effects of the self-esteem and sleeping time, the bootstrapping technique was applied. Life satisfaction, self-esteem and sleeping time decreased as the number of painful stimuli increased. Life satisfaction decreased as self-esteem and sleeping time decreased. On balance, the partial mediation model confirmed that self-esteem and sleeping time both mediate the impact of the number of painful stimuli on life satisfaction.

  5. Randomized controlled comparative study on effect of training to improve lower limb motor paralysis in convalescent patients with post-stroke hemiplegia.

    Science.gov (United States)

    Kawakami, Kenji; Miyasaka, Hiroyuki; Nonoyama, Sayaka; Hayashi, Kazuya; Tonogai, Yusuke; Tanino, Genichi; Wada, Yosuke; Narukawa, Akihisa; Okuyama, Yuko; Tomita, Yutaka; Sonoda, Shigeru

    2015-09-01

    [Purpose] The motor paralysis-improving effect on the hemiplegic lower limb was compared among mirror therapy, integrated volitional-control electrical stimulation, therapeutic electrical stimulation, repetitive facilitative exercises, and the standard training method in post-stroke hemiplegia patients. [Subjects and Methods] Eighty one stroke patients admitted to a convalescent rehabilitation ward were randomly allocated to the above 5 treatment groups. Each patient performed functional training of the paralytic lower limb for 20 minutes a day for 4 weeks, and changes in the lower limb function were investigated using the Stroke Impairment Assessment Set. [Results] The hip and knee joint functions did not significantly improve in the standard training control group, but significant improvements were observed after 4 weeks in the other intervention groups. Significant improvement was noted in the ankle joint function in all groups. [Conclusion] Although the results were influenced by spontaneous recovery and the standard training in the control group, the hip and knee joints were more markedly improved by the interventions in the other 4 groups of patients with moderate paralysis, compared to the control group.

  6. ROLE OF CD95 AND DR3 RECEPTORS IN NA VE T-LYMPHOCYTES APOPTOSIS IN CHILDREN WITH INFECTIOUS MONONUCLEOSIS DURING CONVALESCENCE

    Directory of Open Access Journals (Sweden)

    E. N. Filatova

    2017-01-01

    Full Text Available Infectious mononucleosis is a widespread disease caused by certain members of Herpesviridae family. Acute infectious mononucleosis develops predominantly in children and is accompanied by an increase of the number of circulating naive CD4+ and naive CD8+ T-lymphocytes in the peripheral blood. The normalization of immunological parameters is achieved within 4–6 months after recovery and that is an indicator of a proper functioning of the immune system. CD95 and DR3 death receptors are involved in the initiation of apoptosis of naive T-lymphocytes in healthy people and in patients with infectious mononucleosis. The aim of the study was to evaluate the ability of CD95 and DR3 receptors to initiate apoptosis of naive CD4+ and naive CD8+ T-lymphocytes in children with infectious mononucleosis during convalescence. The material for the study was the samples of the peripheral blood of children who previously had infectious mononucleosis. The blood sampling was carried out again after 4–6 months after the disease. At the time of the study, children did not display clinical and laboratory signs of infectious mononucleosis. Same children who were examined earlier in the period of the development of acute infectious mononucleosis, as well as relatively healthy children were used as the comparison groups. Isolation of naive CD4+ and naive CD8+ T-lymphocytes was performed by negative magnetic immunoseparation. For specific stimulation of CD95 and DR3 receptors monoclonal antibodies were used. The level of apoptosis and expression of death receptors were evaluated by flow cytometry. Freshly isolated cells were analyzed, as well as cells cultured with the addition of appropriate monoclonal antibodies. It was shown that the recovery period was accompanied by increased apoptosis of freshly isolated naive CD4+ and naive CD8+ T-lymphocytes compared with the acute phase of infectious mononucleosis. Thus in both populations of naive T-cells showed an increase of

  7. Correlation between special brain area and blood perfusion in patients with cerebral infarction at convalescent period Feasibility for quantitative determination and estimation of learning and memory function

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    BACKGROUND: Presently, clinic memory scale is used to evaluate learning memory ability in most studies,and the influence of difference in measurement condition of individuals exists.OBJECTIVE: To study the correlation between regional cerebral blood flow (rCBF) perfusion and learning memory function in special brain regions of patients with cerebral infarction at convalescent period,and to try to find out a method which can quantitatively evaluate learning ability.DESIGN: Case observation, and correlation analysis.SETTINGS: Shandong Institute for Behavioral Medicine; the Affiliated Hospital of Jining Medical College.PARTICIPANTS: Totally 70 patients with cerebral infarction admitted to Departtment of Neurology, Jining Medical College between January 2004 and December 2005 were involved. The involved patients, 58 male and 12 female, were averaged (52±3) years, and they were all right handed. They all met the diagnosis criteria instituted by the Fourth National Conference on Cerebrovascular Disease, and were confirmed as cerebral infarction by skull CT or MRI. Informed consents of detected items were obtained from all the patients and relatives.METHODS: When the patients were at convalescent period, their learning and memory ability were measured with" clinic memory scale (set A)". The 18 patients whose total mark over 100 were regarded as good learning memory function group; The 23 cases whose total mark less than 70 were regarded as poor learning memory function group. RCBF of hippocampus, nucleus amygdalae, temporal cortex and prefrontal lobe of patients between two groups were measured and compared by single photon emission computed tomography (SPECT). The total scores of the 18 good learning memory patients and 23 poor learning memory patients were taken as dependent variable Y, and their rCBFs of hippocampus, nucleus amygdale,temporal cortex and prefrontal lobe respectively as independent variable X for linear correlation analysis.MAIN OUTCOME MEASURES

  8. Selection of a rare neutralization-resistant variant following passive transfer of convalescent immune plasma in equine infectious anemia virus-challenged SCID horses.

    Science.gov (United States)

    Taylor, Sandra D; Leib, Steven R; Carpenter, Susan; Mealey, Robert H

    2010-07-01

    Vaccines preventing HIV-1 infection will likely elicit antibodies that neutralize diverse strains. However, the capacity for lentiviruses to escape broadly neutralizing antibodies (NAbs) is not completely understood, nor is it known whether NAbs alone can control heterologous infection. Here, we determined that convalescent immune plasma from a horse persistently infected with equine infectious anemia virus (EIAV) neutralized homologous virus and several envelope variants containing heterologous principal neutralizing domains (PND). Plasma was infused into young horses (foals) affected with severe combined immunodeficiency (SCID), followed by challenge with a homologous EIAV stock. Treated SCID foals were protected against clinical disease, with complete prevention of infection occurring in one foal. In three SCID foals, a novel neutralization-resistant variant arose that was found to preexist at a low frequency in the challenge inoculum. In contrast, SCID foals infused with nonimmune plasma developed acute disease associated with high levels of the predominant challenge virus. Following transfer to an immunocompetent horse, the neutralization-resistant variant induced a single febrile episode and was subsequently controlled in the absence of type-specific NAb. Long-term control was associated with the presence of cytotoxic T lymphocytes (CTL). Our results demonstrate that immune plasma with neutralizing activity against heterologous PND variants can prevent lentivirus infection and clinical disease in the complete absence of T cells. Importantly, however, rare neutralization-resistant envelope variants can replicate in vivo under relatively broad selection pressure, highlighting the need for protective lentivirus vaccines to elicit NAb responses with increased breadth and potency and/or CTL that target conserved epitopes.

  9. Comparison of acute versus convalescent stage high-sensitivity C-Reactive protein level in predicting clinical outcome after acute ischemic stroke and impact of erythropoietin

    Directory of Open Access Journals (Sweden)

    Yeh Kuo-Ho

    2012-01-01

    Full Text Available Abstract Background and Aim Currently, no data on the optimal time point after acute ischemic stroke (IS at which high-sensitivity C-reactive protein (hs-CRP level is most predictive of unfavorable outcome. We tested the hypothesis that hs-CRP levels during both acute (48 h after IS and convalescent (21 days after IS phases are equally important in predicting 90-day clinical outcome after acute IS. We further evaluated the impact of erythropoietin (EPO, an anti-inflammatory agent, on level of hs-CRP after acute IS. Methods Totally 160 patients were prospectively randomized to receive either EPO therapy (group 1, n = 80 (5,000 IU each time, subcutaneously at 48 h and 72 h after acute IS, or placebo (group 2, n = 80. Serum level of hs-CRP was determined using ELISA at 48 h and on day 21 after IS and once in 60 healthy volunteers. Results Serum level of hs-CRP was substantially higher in all patients with IS than in healthy controls at 48 h and day 21 after IS (all p 0.5. Multivariate analysis showed that hs-CRP levels (at 48 h and day 21 were independently predictive of 90-day major adverse neurological event (MANE (defined as recurrent stroke, NIHSS≥8, or death (all p Conclusion EPO therapy which was independently predictive of freedom from 90-day MANE did not alter the crucial role of hs-CRP levels measured at 48 h and 21-day in predicting unfavorable clinical outcome after IS.

  10. Cross-reactive antibodies in convalescent SARS patients' sera against the emerging novel human coronavirus EMC (2012) by both immunofluorescent and neutralizing antibody tests.

    Science.gov (United States)

    Chan, Kwok-Hung; Chan, Jasper Fuk-Woo; Tse, Herman; Chen, Honglin; Lau, Candy Choi-Yi; Cai, Jian-Piao; Tsang, Alan Ka-Lun; Xiao, Xincai; To, Kelvin Kai-Wang; Lau, Susanna Kar-Pui; Woo, Patrick Chiu-Yat; Zheng, Bo-Jiang; Wang, Ming; Yuen, Kwok-Yung

    2013-08-01

    A severe acute respiratory syndrome (SARS)-like disease due to a novel betacoronavirus, human coronavirus EMC (HCoV-EMC), has emerged recently. HCoV-EMC is phylogenetically closely related to Tylonycteris-bat-coronavirus-HKU4 and Pipistrellus-bat-coronavirus-HKU5 in Hong Kong. We conducted a seroprevalence study on archived sera from 94 game-food animal handlers at a wild life market, 28 SARS patients, and 152 healthy blood donors in Southern China to assess the zoonotic potential and evidence for intrusion of HCoV-EMC and related viruses into humans. Anti-HCoV-EMC and anti-SARS-CoV antibodies were detected using screening indirect immunofluorescence (IF) and confirmatory neutralizing antibody tests. Two (2.1%) animal handlers had IF antibody titer of ≥ 1:20 against both HCoV-EMC and SARS-CoV with neutralizing antibody titer of SARS patients had significant IF antibody titers with 7/28 (25%) having anti-HCoV-EMC neutralizing antibodies at low titers which significantly correlated with that of HCoV-OC43. Bioinformatics analysis demonstrated a significant B-cell epitope overlapping the heptad repeat-2 region of Spike protein. Virulence of SARS-CoV over other betacoronaviruses may boost cross-reactive neutralizing antibodies against other betacoronaviruses. Convalescent SARS sera may contain cross-reactive antibodies against other betacoronaviruses and confound seroprevalence study for HCoV-EMC. Copyright © 2013 The British Infection Association. Published by Elsevier Ltd. All rights reserved.

  11. Surveys of requirement status of work and recreational therapy in convalescence psychosis patients%康复期精神病患者对工娱疗活动的需求状况调查

    Institute of Scientific and Technical Information of China (English)

    郭卫东; 郝花; 郝鹏

    2014-01-01

    目的:了解康复期精神病患者对工娱疗活动的需求状况,为康复活动的开展提供依据。方法采用自制精神病患者工娱疗活动需求调查表对80例康复期精神病患者进行调查分析。结果本组康复期精神病患者对各项工娱疗活动需求率均≥48.8%,其中对音乐放松疗法和抗复发训练的需求率分别为76.3%、75.0%。结论康复期精神病患者对工娱疗活动的需求较高,有针对性地开展康复项目,能最大限度地提高患者的社会生活技能,对促进患者早日回归社会,具有重要作用。%Objective To study the requirement status of work and recreational therapy in convalescence psychosis patients in order to provide basis for develo-ping rehabilitation activities .Methods Assessments were conducted with the Self-made Questionnaire for Psychotic Requirement of Work and Recreational Therapy in 80 convalescence psychosis patients .Results Their need rates for all work and recreational activities were ≥48 .8% ,those for music relaxation therapy and anti-relapse training respectively 76 .3% and 75 .0% .Conclusion Needs for work and recreational ac-tivities are higher in convalescence psychosis patients , purposeful rehabilitation items can improve pa-tients’ social life skills maximally and promote patients’ return to society as early as possible .

  12. The influence of comprehensive nursing on life quality of patients with schizophrenia in convalescence%综合护理模式对康复期精神分裂症患者生活质量的影响

    Institute of Scientific and Technical Information of China (English)

    冯锦妹

    2011-01-01

    Objective: To explore the influence of comprehensive nursing on life quality of patients with schizophrenia in convalescence.Methods :68 cases of patients with schizophrenia in convalescence were divided into experimental group and control group, control group was given routine nursing, and experimental group was given to comprehensive nursing.Results: The score of PANSSE and GQOLI in experimental group was significantly better than that in control group,it showed better clinical nursing effect Conclusion: Comprehensive nursing used for patients with schizophrenia in convalescence, can improve the disease and enhance the life quality significantly, so it is one of the desirable nursing models.%目的:探讨综合护理模式对康复期精神分裂症患者生活质量的影响.方法:将68例康复期精神分裂症患者随机分为实验组35例和对照组33例.对照组给予常规护理,实验组给予综合护理.结果:实验组在PANSSE评分和GQOLI评分方面均显著优于对照组,显示了更好的护理效果.结论:综合护理模式应用于精神分裂症患者,对于改善病情,提高生活质量是很有意义的,是值得在临床上考虑的护理模式之一.

  13. T-Regulatory Cells and Inflammatory and Inhibitory Cytokines in Malawian Children Residing in an Area of High and an Area of Low Malaria Transmission During Acute Uncomplicated Malaria and in Convalescence.

    Science.gov (United States)

    Nyirenda, Tonney S; Molyneux, Malcolm E; Kenefeck, Rupert; Walker, Lucy S K; MacLennan, Calman A; Heyderman, Robert S; Mandala, Wilson L

    2015-09-01

    Malaria still infects many Malawian children, and it is a cause of death in some of them. Regulatory T cells (Tregs) help in negating immune-related pathology, it but can also favor multiplication of malaria parasites. The question remains whether children recovering from uncomplicated malaria (UCM) have higher Tregs and interleukin (IL)-10 levels in convalescence. We recruited children between the ages of 6 and 60 months presenting with acute UCM in Blantyre (low transmission area) and Chikwawa (high transmission area). We observed the children after 1 month and 3 months and analyzed their blood samples for parasitemia, lymphocyte subsets, and levels of the cytokines interferon (IFN)-γ, IL-10, and transforming growth factor (TGF)-β. Blood samples from age-matched controls were also analyzed for the same parameters. Compared with controls, acute UCM was associated with mild lymphopenia, splenomegaly, and high levels of IFN-γ, tumor necrosis factor-α, and IL-10, which normalized in convalescence. In Chikwawa, Treg counts were significantly (P effect on parasite clearance. We recommend that children recovering from UCM, especially those from high transmission area, should sleep under insecticide-treated nets, be screened for parasitemia, and a provision of antimalarial prophylaxis should be considered.

  14. Influence of motivational interview on negative emotion of convalescence schizophrenia patients%动机性访谈对恢复期精神分裂症患者负性情绪的影响

    Institute of Scientific and Technical Information of China (English)

    王玉春

    2015-01-01

    目的:探讨动机性访谈对恢复期精神分裂症患者负性情绪的影响,以帮助患者消除负性情绪,全面康复,顺利回归社会。方法100例恢复期精神分裂症患者,按入组先后顺序随机分为实验组和对照组,各50例,两组均接受抗精神病药物系统治疗,对照组给予精神科护理常规及心理护理,实验组患者在此基础上每周至少访谈1次,连续8周,两组患者在访谈前后分别采用焦虑自评量表(SAS)、抑郁自评量表(SDS)评定评分。结果访谈8周后两组患者评定评分均有变化,实验组患者变化更明显(P<0.05)。结论动机性访谈能有效消除恢复期精神分裂症患者的负性情绪,促进患者的全面康复。%Objective To investigate the influence of motivational interview on negative emotion of convalescence schizophrenia patients, in order to help them eliminate negative emotion, gain comprehensive rehabilitation and return to society.Methods A total of 100 convalescence schizophrenia patients were randomly divided into experimental group and control group, with 50 cases in each group. Both groups received systematic treatment by antipsychotics, conventional psychiatric and mental nursing. The experimental group received additional interview at least once a week for 8 weeks. Self rating anxiety scale (SAS) and self rating depression scale (SDS) were applied for both groups before and after interview.Results After 8 weeks of interview, both groups had changes in evaluation scores, and the experimental group had much more obvious changes (P<0.05). Conclusion Motivational interview can effectively eliminate negative emotion of convalescence schizophrenia patients and promote their comprehensive rehabilitation.

  15. 音乐治疗对精神分裂症康复期患者认知功能的影响%Effect of Music Therapy on Cognitive Function in Convalescent Patients with Schizophrenia

    Institute of Scientific and Technical Information of China (English)

    梁飞平; 袁佩; 马沛珍

    2014-01-01

    Objective To explore the effect of music therapy on cognitive function in convales-cent patients with schizophrenia.Methods Eighty convalescent patients with schizophrenia were randomly divided into study group and control group,with 40 patients in each group.All patients were treated with antipsychotic medications.In addition,patients in study group received music therapy.Curative efficacy was evaluated using Wisconsin Card Sorting Test(WCST),Wechsler Memory Scale(WMS)and Wechsler Adult Intelligence Scale(WAIS-R).Results Compared with control group,music therapy significantly increased WCST scores of completed categories and correct responses,WMS scores of short-term memory and immediate memory and WAIS-R scores of performance IQ,but obviously decreased WCST scores of perseverative responses after treat-ment for 6 months(P<0.05 or P<0.01).Conclusion Music therapy can improve the cognitive function in convalescent patients with schizophrenia.%目的:探讨音乐治疗对精神分裂症康复期患者认知功能的影响。方法将80例精神分裂症康复期患者按随机数字表法分为研究组和对照组,每组40例,2组患者均按精神科常规治疗,研究组在此基础上进行音乐治疗。以威斯康星卡片分类测验(WCST)、韦氏记忆量表(WMS)、韦氏成人智力量表(WAIS-R)进行效果评定。结果治疗6个月后,研究组 WCST评分正确应答数和完成分类数明显高于对照组(P<0.05),持续性应答数较对照组显著降低(P<0.01),WMS短时记忆、瞬时记忆得分明显高于对照组(P<0.05),WAIS-R操作智商明显高于对照组(P<0.05)。结论音乐治疗能改善精神分裂症康复期患者的认知功能。

  16. Effect of Low Mountain Climate Convalescence Nursing on the Psychosomatic Health of the Eld-erly Hypertensive Patients%低山带疗养护理对老年高血压患者心身健康的影响

    Institute of Scientific and Technical Information of China (English)

    赵晓红; 姚萍; 曹莉芬; 李清红; 朱开梅; 冯小力

    2014-01-01

    Objective To discuss the effect of low mountain climate convalescence nursing on psychoso-matic health status of elderly hypertensive patients.Methods 68 elderly hypertensive patients nursing in Jigong Mountain from July to September in 2012 were selected.Blood pressure,heart rate,oxyhemoglobin saturation,body mass index and psychological health status before and after the convalescence were investi-gated and compared respectively.Results After the convalescence,systolic pressure and oxyhemoglobin saturation were all better than before convalescence (all P 0.05). There were significant differences of the scores of somatization,depression,anxiety,phobic anxiety and sleeping and eating disorders compared with pre-convalescence (all P 0.05).Conclusion Convalescence in low mountain climate has a positive effect on the blood pressure and the psychosomatic health status in elderly hypertensive patients.%目的:探讨低山带疗养护理对老年高血压患者心身健康的影响.方法2012年7-9月,广州军区武汉疗养院对在鸡公山疗养地疗养的68名老年高血压患者疗养前后的血压、心率、血氧饱和度、体质指数及心理健康状况等进行了研究并比较.结果疗养后,老年高血压患者的收缩压及血氧饱和度均优于疗养前(均 P <0.01),舒张压、心率、BMI 等与疗养前比较,差异均无统计学意义(均 P >0.05);躯体化、抑郁、焦虑、恐怖、睡眠饮食障碍等5项因子均分与疗养前的差异均有统计学意义(均 P <0.05),其余因子的评分与疗养前的差异均无统计学意义(均 P >0.05).结论低山带气候疗养护理对老年高血压患者的血压及心身健康状况有积极的影响.

  17. Application of Structured Group Psychotherapy in Convalescent Schizophrenics%结构式团体心理治疗在精神分裂症康复期的应用研究

    Institute of Scientific and Technical Information of China (English)

    曹民佑; 卢惠鹏; 吴虹; 杨志辉; 梁飞平; 胡玲玲

    2014-01-01

    Objective To study the effect of structured group psychotherapy on overall social functions of convalescent schizophrenics.Methods A total of 120 convalescent schizophrenics were randomly divided into two groups,with 60 patients in each group.Patients in control group received drug treatment and rehabilitation training.On this basis,patients in study group were given structured group psychotherapy once per week for 10 week (1 hour per week).Patients were evaluated by using Scale of Social-Skills for Psychiatric Inpatients (SSSI),Self-Esteem Scale (SES)and Self Consistency and Congruence Scale (SCCS)before treatment,after treatment and at 12 weeks of follow-up.Results After treatment,SES scores significantly increased in study group but obviously decreased in control group(P 0.05).Furthermore,there were sig-nificant differences in SSI,SES and SCCS between the two groups after treatment(P 0.05).Conclusion Structured group psychotherapy can stably and durably improve the social function of convalescent schizophrenics.%目的:研究结构式团体心理治疗对康复期精神分裂症患者整体社会功能的影响。方法将120例康复期精神分裂症患者按简单随机方法分成2组,每组60例。研究组在原有药物治疗及康复训练基础上实施结构式团体心理治疗,每周1次,每次1 h,共10次;对照组则维持原有药物治疗及康复训练。分别在治疗前后及随访12周时,使用住院慢性精神病患者社会功能评定量表(SSSI)、自尊量表(SES)、自我和谐量表(SCCS)进行评估。结果研究组治疗后 SES 总分高于治疗前,SSSI、SCCS 总分低于治疗前(均 P <0.05)。对照组治疗后 SES 总分低于治疗前(P <0.05),而治疗前后 SSSI 和 SCCS 总分差异无统计学意义(均 P >0.05)。2组治疗后 SSSI、SES、SCCS总分差异均有统计学意义(均 P <0.05)。研究组12周随访时与治疗后 SSSI、SES、SCCS 总分比

  18. 心理干预对康复期精神分裂症患者康复效果的影响%The effect of psychological intervention on rehabilitation out-come of convalescence schizophrenia patients

    Institute of Scientific and Technical Information of China (English)

    崔永霞; 王灵灵

    2015-01-01

    目的:探讨心理干预对康复期精神分裂症患者康复效果的影响。方法将60例康复期精神分裂症患者随机分为两组,均予以常规抗精神病药物治疗及护理,研究组在此基础上联合心理干预。观察2个月。采用简明精神病量表、住院精神病人康复疗效评定量表评定临床疗效。结果心理干预2个月后两组简明精神病量表总分和住院精神病人康复疗效评定量表总分及各因子分均较干预前显著降低(P<0.05或0.01),研究组显著低于对照组(P<0.01)。结论心理干预可有效提高康复期精神分裂症患者的生活能力及社交能力,延缓患者的精神衰退,有利于促进患者全面康复。%Objective To explore the effect of psychological intervention on rehabilitation outcome of con‐valescence schizophrenia patients .Methods Sixty convalescence schizophrenics were randomly assigned to two groups ,both groups received routine antipsychotic treatment and nursing ,on this basis research group was plus mental intervention for 2 months .Efficacies were assessed with the Brief Psychiatric Rat‐ing Scale (BPRS) and Inpatient Psychiatric Rehabilitation Outcome Scale (IPROS) .Results The total and each factor scores of both groups lowered more significantly after 2 month intervention compared with preintervention (P<0 .05 or 0 .01) ,so did those in research than in control group (P<0 .01) .Conclusion Psychological interventions could effectively improve viability and sociability of convalescence schizophrenia patients ,postpone patients’ mental deterioration ,and are beneficial to promote patients’ comprehensive rehabilitation .

  19. The clinical observation of telmisartan combined kidney convalescent to treat diabetic nephropathy%替米沙坦联合肾复康片治疗糖尿病肾病的临床观察

    Institute of Scientific and Technical Information of China (English)

    高君

    2013-01-01

    Objective Analyze and study the curative effect of telmisartan combined kidney convalescent to treat diabetic nephropathy .Method Choose 160 cases of patients with high blood pressure at our hospital,according to the different method of treatment,they will be divided into two groups.Treatment with the control group only telmisartan,kidney within the experimental group is used with telmisartan combination therapy.Compare and observe effect of two groups of patients after treatment.Re-sult Experimental group were controlled by various indicators total efficiency(87.50%)was significantly higher(57.50%),statistically significant(P<0.05).Conclu-sion Using renal convalescent piece with telmisartan approach in the treatment of diabetic nephropathy have important applied value ,can effectively prevent the develop-ment of diabetic nephropathy,promote the recovery of patients with renal function,and less adverse reaction,can be widely used in clinical.%目的:关于将肾复康片与替米沙坦联合用于治疗糖尿病肾病所取得的疗效进行分析研究。方法选取到我院接受治疗的患有高血压的患者160例,按治疗方法的不同将其分成两组。对照组仅采用替米沙坦治疗,实验组则采用肾复康片与替米沙坦联合治疗。比较并观察两组患者经治疗后取得的效果。结果实验组患者各项指标控制的总有效率(87.50%)明显高于对照组(57.50%),统计学上有意义(P<0.05)。结论采用肾复康片与替米沙坦联合的方式在糖尿病肾病的治疗中有重要的应用价值,能有效阻止糖尿病肾病的发展,促进患者肾功能的恢复,且不良反应少,在临床上可以广泛运用。

  20. A Controlled Study of Bibliotherapy on Social Function of Schizophrenia Patients at Convalescent Stage%阅读疗法对恢复期精神分裂症患者社会功能的影响

    Institute of Scientific and Technical Information of China (English)

    李尚兰

    2011-01-01

    目的 探讨阅读疗法对恢复期精神分裂症患者社会功能的影响.方法 在药物治疗的基础上,观察组60例恢复期精神分裂症患者实施阅读疗法,对照组60例恢复期精神分裂症患者给予一般的心理支持治疗.两组患者分别于入组时、入组8周时、入组16周时用康复状态量表(MRSS)、社会功能缺陷筛选量表(SDSS)、护士用住院患者观察量表(NOSIE-30)、日常生活能力量表(ADL)进行社会功能评定.结果 入组时两组间MRSS、NOSIE-30、SDSS、ADL评分比较,差异无统计学意义(P>0.05);入组8周时观察组MRSS、NOSIE-30、SDSS、ADL评分与对照组比较,差异有统计学意义(P<0.05或P<0.01);入组16周时观察组MRSS、NOSIE-30、SDSS、ADL评分与对照组比较,差异有统计学意义(P<0.01).结论 阅读疗法对恢复期精神分裂症患者社会功能的改善效果肯定,是精神分裂症患者回归社会的有效途径.%Objective To explore the effects of bibliotherapy on the social function of schizophrenia patients at convalescent stage. Methods Both groups maintained antipsychotic treatment,the research group (60 cases) received additional bibliotherapy,while the control received common psychotherapy both for 3 months;MRSS, SDSS,NOSIE-30 and ADL were used to evaluate social function before and after 8,16-weeks treatment respectively. Results Between the two groups, the scores of MRSS, SDSS,NOSIE-30 and ADL had no significant difference before treatment(P > 0. 05), but had significant difference after the treatment of 16 weeks( P < 0. 0l ). Conclusion Bibliotherapy can improve the social function of the patients with schizophrenia at convalescent stage.

  1. 烧伤康复期患者韧性水平与生存质量的相关性研究%Research in relationship of the quality of life and resilience level of burn patients during convalescence

    Institute of Scientific and Technical Information of China (English)

    杨珍; 王君俏; 张宝珍; 曾玉

    2013-01-01

    目的 了解烧伤康复期患者韧性水平与患者生存质量之间的相关性,为烧伤患者的心理危机预防和干预提供理论指导.方法 对255例烧伤康复期患者的一般资料、韧性、生存质量进行问卷调查.结果 烧伤患者韧性得分处于中等水平,生存质量水平处于中等偏低水平,烧伤康复期患者的韧性水平与生活质量之间存在显著正相关,韧性水平对患者心理健康水平影响较大,韧性中的坚韧与力量因子得分与心理健康得分成显著正相关.结论 应通过干预来帮助人们提高韧性,达到发展潜能,预防行为障碍的效果,帮助患者提高韧性从而产生正向的心理适应能力.%Objective To understand the level of resilience and quality of life of burn patients during convalescence,to provide theoretical guidance for burn patients' psychological crisis prevention and intervention.Methods 255 cases of burn patients during convalescence were interviewed with general status questionnaire,CD-RISC,and burn specific health scale(BSHS).Results The resilience score of burn patients was at the middle level,the level of quality of life was at the lower-middle-level,significant positive correlation was found between the level of resilience and quality of life.Resilience level had a greater impact on the level of mental health in burn patients.The factors of physical strength and tenacity of resilience scale were positively correlated with mental health.Conclusions Intervention should be given to help people improve resilience,in order to reach the effect of potential development and prevention of behavior dysfunction,so that help them engender positive psychological adjustment.

  2. 社区护理对脑出血康复期患者便秘的影响%The Effect of Community Nursing on Constipation of Patients With Cerebral Hemorrhage During Convalescence

    Institute of Scientific and Technical Information of China (English)

    马艳萍

    2015-01-01

    目的:探讨社区护理对脑出血康复期患者便秘的影响。方法对154例患者采取包括运动指导,饮食指导和用药指导等有针对性的护理措施。结果154例患者中,大便通畅自行排便1次/d有78例,排便变顺利59例,需人工掏便15例,因排便用力引起再出血2例。结论各种护理措施的综合应用可以帮助患者建立正常的排便行为,提高生存质量。%Objective To discuss the effect of community nursing on constipation of patients with cerebral hemorrhage during convalescence. Methods To take targeted nursing measures on 154 cases of patients, including sports, dietary and drug guidance. Results In 154 patients, the stool unobstructed in 78 cases, defecation smoothly in 59 cases, to dig out for 15 cases, due to defecation and forced bleeding in 2 cases. Conclusion The comprehensive application of various nursing measures can help patients establish normal defecation behavior and improve the quality of life.

  3. Characteristics of mood disturbance in convalescent stage of senile cerebral infarct%老年脑梗死患者恢复期情绪障碍的特征

    Institute of Scientific and Technical Information of China (English)

    吕永良; 钱连华; 朱伟; 沈明强; 周宝娣

    2004-01-01

    背景:脑卒中后情绪障碍以焦虑、抑郁常见,但是有关老年脑梗死患者恢复期情绪障碍的研究报告较少.目的:探讨老年脑梗死患者恢复期情绪障碍的特点以及情绪障碍与神经功能障碍的关系.设计:病例-对照研究.单位:苏州市广济医院精神科和苏州市第二人民医院神经内科.对象:2001-05/2003-06苏州市第二人民医院神经内科住院后出院的患者87例(病例组),男48例,女39例,年龄61~84岁.均首次发病,病程在第3个月的恢复期者.选择本院健康体检人员68例为对照组,神经系统检查和躯体检查正常,无精神病史,无痴呆表现,男39例,女29例,年龄60~82岁.方法:根据统一的指导语,在病程第3个月完成症状自评量表(SCL-90)和改良爱丁堡-斯堪的那维亚量表(SSS)评定,一致性检验Kappa值为0.82.主要观察指标:①SCL-90总分和因子分.②SSS总分.结果:病例组SCL-90总分、阳性项目、阳性总分、阳性均分均高于对照组(t=4.14~8.09,P=0.000).病例组SCL-90躯体化因子、强迫因子、人际关系因子、抑郁因子、焦虑因子、恐怖因子、精神病性因子评分高于对照组(u=2.107~8.811,P=0.000).两组在敌对和偏执因子的差异无统计学意义(P>0.05).病例组SCL-90总分及因子分与神经功能缺损总分呈正相关(r=0.806~0.293,P<0.01).结论:老年脑梗死患者恢复期常见抑郁、焦虑和恐怖等情绪障碍.其情绪障碍与神经功能缺损的严重程度有关.%BACKGROUND: Anxiety and depression are common in mood disturbance after cerebral apoplexy. However, there are few researches about mood disturbance in convalescent stage of senile cerebral infarct.OBJECTIVE: To investigate the characteristics of mood disturbance in convalescent stage of senile cerebral infarct and the relationship between mood disturbance and neural dysfunction.DESIGN: A case-controlled trial.SETTING: Department of Psychiatrics, Guangji Hospital and

  4. 24式太极拳锻炼对功能性便秘患者的康复影响%About the convalescent influence of 24-Pattern Shadowboxing to the patients with chronic functional constipation

    Institute of Scientific and Technical Information of China (English)

    周勇; 梁磊; 李虹; 李靖

    2015-01-01

    目的:探讨24式太极拳锻炼对功能性便秘患者肠胃功能的康复影响。方法:在陕西省中医院、第四军医大学西京医院、陕西省人民医院随机抽选自愿参加实验的无运动禁忌的功能性便秘患者51例,进行为期3个月的实验研究;将51例患者随机分为A、B、C三组,A组为太极拳锻炼组,B组为药物治疗组,C组为对照组。结果:A、B组患者的肠胃功能在实验前后具有明显的改善(p0.05);实验后A组患者比B、C两组有显著改善(p<0.01)。结论:虽然药物治疗对功能性便秘患者的肠胃功能状态的改善有一定的效果,但相对于24式太极拳锻炼,其改善程度并不明显,表明24式太极拳锻炼对功能性便秘患者肠胃功能状态的改善具有积极的康复影响。%Aim:the article discusses the convalescent influence of 24-Pattern Shadowboxing to the patients with chronic functional constipation. Method: random selects 51 patients with chronic functional constipation from Chinese medicinehospitals, Xijing Hospital of Fourth Military Medical University and People Hospital of Shaanxi,and divides them into three groups(group A is shadowboxing group,group B is drug therapy group, group C is control group) to experimental survey for 3 months. Result: it has a very obvious improvement for gastrointestinal functions of group A and B before and after experiment,gastrointestinal functions of group C has little symptomatic improvement or exacerbation,and group has more obvious improvement than B and C after experiment. Conclusion:compared to 24-Pattern Shadowboxing,drug therapy has less improvement even though it has some effect for gastrointestinal functions of patients with chronic functional constipation which testifies 24-Pattern Shadowboxing has positive convalescent influence for the improvement of gastrointestinal functions of patients with chronic functional constipation.

  5. A study of the stigma experience and the different influencing factors in convalescent patients with schizophrenia%恢复期精神分裂症的病耻感及影响因素研究

    Institute of Scientific and Technical Information of China (English)

    蔡端芳; 龙赟; 童颜; 蒋特; 谭友果; 刘敏; 王慢利; 李杨

    2012-01-01

    目的 研究恢复期精神分裂症患者的病耻感体验及不同影响因素.方法 以符合ICD-10精神分裂症诊断标准,入院治疗6周以上,年龄18~45岁,GAF≥61分97例患者作为调查对象.选择“精神病患者病耻感评估量表”作为评定工具,分析BPRS、GAF与病耻感的相关性,比较各种因素对病耻感的影响.结果 治疗6周后的病耻感与BPRS、SGAF评分有相关性(P分别为0.05、0.10).在精神分裂症不同亚型比较中仅反映出能力因子差异有统计学意义(P<0.05),且多反映在青春型、紧张型与未定型之间.随着病程延长,病耻感总分、各因子分都出现了有意义的下降(P<0.05).各因素比较中,女性总分和各因子分均非常显著高于男性(P<0.01).结论 恢复期精神分裂症患者病耻感体验女性患者比男性明显,但随着病程延长,病耻感体验显示出了轻度化状态.%To study the stigma experience and the different influencing factors in convalescent patients with Schizophrenia. Methods Ninety-seven patients were investigated, who matched with the standard of diagnosis of ICD-10 Schizophrenia, and their scores of GAF are more than 61, then accessed by Stigma Scale for Mental Illness, analyzed the relativity of the scores of BPRS.GAF and stigma after 6 weeks of treatment, compared impact of the different factors on stigma. Results There is a positive relationship between stigma and the scores of BPRS( P = 0.05 ) , and GAF( P = 0. 1). Only the behavior ability shows significant difference in subtypes of the Schizophrenia patients (P < 0. 05 ) , mostly reflected in the adolescent type , tense type and the unshaped . Following the course of disease, the total scores and the various factorial scores of stigma decreased significantly. Compared with the different factors , the total scores and the various factorial scores in female are significantly higher than those in maIe(P <0. 01), and the scores of those living in urban are

  6. Application of gait training based on Bobath concept framework in rehabilitation of stroke patients in convalescent phase%基于Bobath理念的步行训练在恢复期脑卒中患者中的应用

    Institute of Scientific and Technical Information of China (English)

    王文威; 王路; 李凌晋; 石新棉; 林冠梁; 杜志伟; 曾祥城; 潘翠环

    2012-01-01

    目的:观察基于Bobath理念的步行训练对恢复期脑卒中患者的步行功能的疗效.方法:80例具有步行功能障碍的脑卒中恢复期患者随机分为Bobath组(n=40),对照组(n=40).Bobath组接受一套基于Bobath理念理论框架下的步态训练法.对照组接受常规的步行训练.两组患者步行训练时间均为30min/d,5d/周,持续4周,其余康复治疗如作业疗法、物理因子治疗等两组均相同.两组患者分别于治疗前、治疗后及出院后3周予以FMA下肢(FMA-LE)运动功能评定、Berg平衡量表(BBS)进行评定,同时选用足印步态分析法测量患者的步行参数变化.结果:两组患者治疗后FMA-LE和BBS的评分均较治疗前提高,步行参数(平均步长、步宽及步速)得到改善,治疗前、后各量表的评分差异具有显著性(P<0.05);与对照组相比,Bobath组患者在各量表评分提高幅度更大(P<0.05).两组患者在出院后3周再次评定时,组内比较各量表评分均较治疗后有所提高,但Bobath组内评分比较具有显著性(P<0.05),对照组内比较无显著性(P>0.05).结论:对于步行功能障碍的脑卒中患者,采用基于Bobath理念的步行训练法可以更好的改善其步行功能,并且疗效具有持续性.%Objective: To observe the effects of gait training based on Bobath concept framework intervening on walking capability of stroke patients in convalescent phase. Method: Eighty subjects participated in this randomized single-blind study. Subjects in Bobath group (n=40) practiced with gait training based on Bobath concept framework, and control group (n=40) performed conventional gait training. Program of gait training for both groups were 30 min per day, 5d per week for 4 weeks. Meanwhile, the other rehabilitation therapy programs (occupational therapy, physical modalities therapy) were the same for both group. Fugl-Meyer assessment of lower extremity (FMA-LE), Berg balance scale(BBS), and foot-print analysis

  7. 军队疗养青年军人睡眠质量调查及其影响因素分析%Investigation of sleep quality and its influential factors in Chinese young convalescent soliders

    Institute of Scientific and Technical Information of China (English)

    陈素娟; 张瑞乔; 张林基; 张筱微; 陈宇

    2013-01-01

    Objective: To observe the status of the sleep quality of the Chinese young convalescent soliders and analyze its influential factors, and to provide reference for corresponding health care and health education. Methods: A total of 346 young military persons were investigated by the Pittsburgh sleep quality index (PSQI) scale in our sanatorium. The individual information and potential influential factors related with sleep quality were collected. Results; The total sleep quality of young military personnel was not good. The PSQI score was 7. 12 ± 3.76. The chronic diseases and special service environment could influence the sleep quality. Conclusion; The status of sleep quality of young military personnel should not be neglected, and the necessary interventional and therapeutic measures should be taken.%目的:了解青年军人的睡眠质量,并分析影响睡眠质量的因素,为制定相应的医疗保健及健康教育措施提供参考.方法:采用匹兹堡睡眠质量指数(PSQI)量表对来我院疗养的346名青年军人进行调查,并分析可能影响睡眠质量的因素.结果:青年军人总体睡眠状况一般,PSQI得分平均为(7.12±3.76)分.一些慢性疾病或特殊服役环境会影响睡眠质量.结论:青年军人睡眠质量问题不容忽视,需要采取必要的措施进行干预和治疗.

  8. Personality Characteristics and Mental Health of Patients with Schizophrenia of Primary Affection and Convalescent Stage%首发、恢复期精神分裂症患者人格特征与心理健康的研究

    Institute of Scientific and Technical Information of China (English)

    刘竹华; 张建新; 周明洁; 王力

    2011-01-01

    目的 探讨首发、恢复期精神分裂症患者的人格特征、心理健康及两者的相关性,为进一步制定首发、恢复期精神分裂症患者的综合康复计划提供理论依据.方法 采用McCrae和Costa编制的简易人格问卷(NEO-FFI)及中国人身心健康综合评估与诊断系统中的心理健康部分为测评工具,对79例经新型非典型抗精神病药治疗、首发、恢复期的门诊及住院精神分裂症患者(病例组)和169例健康人(正常对照组)的人格特征、心理健康状况及两者的相关性进行比较.结果 病例组与正常对照组的人格特征:神经质[(3.06±0.55)分vs.(2.72±0.54)分]、外向性[(3.01±0.46)分vs.(3.29±0.41)分]、宜人性[(3.27±0.30)分vs.(3.50±0.33)分]、责任心[(3.20±0.44)分vs.(3.45±0.35)分]得分比较,差异均有统计学意义(P<0.001),开放性[(3.13±0.40)分vs.(3.09±0.37)分]得分比较,差异无统计学意义(P>0.05).病例组与正常对照组的心理健康:正性情绪[(1.62±0.91)分vs.(2.27±0.69)分]、认知功能[(1.28±0.71)分vs.(1.10±0.63)分]、自我评价[(2.19±0.98)分vs.(1.67±0.82)分]得分比较,差异均有统计学意义(P<0.05).负性情绪[(1.34±0.86)分vs.(1.18±0.72)分]、行为问题[(0.99±0.54)分vs.(0.94±0.48)分]得分比较,差异均无统计学意义(P>0.05).病例组人格特征与心理健康关系密切,其中神经质与负性情绪、行为问题、自我评价正相关(P<0.001),外向性与正性情绪正相关(P<0.05).结论 制定首发、恢复期精神分裂症患者的综合康复计划时,必须积极关注其个性特征及心理健康状况,尽可能减少个性特征的某些特质对心理健康的影响.%Objective To explore the personality characteristics and mental health of patients with schizophrenia of primary affection and convalescent stage, as well as the correlation between the two, so as to provide the theoretical basis for further formulating a comprehensive

  9. 多种针法联合治疗卒中恢复期吞咽困难疗效观察%Effect of Acupuncture on Dysphagia of Convalescent Stroke Patients XIE

    Institute of Scientific and Technical Information of China (English)

    解越; 刘泓; 周炜; 王丽平; 郑燕鸿; 张树源; 李志亮

    2011-01-01

    目的 探讨体针、头皮针加电针联合应用对卒中恢复期吞咽困难患者的吞咽功能改善是否优于常规康复训练.方法 将148例卒中恢复期吞咽困难的患者随机分配至针刺组和康复训练组,每组74例.针刺组取患侧风池、完骨、翳风、廉泉、金津、玉液穴;头皮针围刺;体针、头皮针加电针联合应用,每天针刺1次,每周5次为1个疗程,共2~4个疗程.对照组由语言治疗师进行康复训练.治疗后3~6个月对主要结局同时使用意向性分析和符合方案数据分析两种方法评价,并报告"为了避免 1 例不良事件发生必须治疗的患者数(number needed to treat,NNT)"、"处理多少病例可以引起1次副作用(number needed to harm,NNH)"及其95%的可信区间.治疗结束时评价次要结局.结果 治疗结束时和治疗后3个月针刺组洼田吞咽功能痊愈率、洼田饮水试验痊愈率优于康复训练组,差异有统计学意义(P<0.05).治疗结束时肺部感染率和病死率、治疗后6个月病死率两组比较差异无统计学意义(P>0.05).两组依从性均为100%,针刺组未出现不良反应.结论 针刺对卒中恢复期吞咽困难患者的吞咽功能改善优于常规康复训练,且安全、耐受性好.%Objective To discuss the therapeutic effect on dysphagia of the convalescent stroke by combination of the body acupuncture, the scalp acupuncture and the electric acupuncture could be better than that by routine rehabilitation training.Methods 148 patients with dysphagia of convalescent stroke were randomly assigned to the acupuncture group and rehabilitation group, 74 in each group.Fengchi (GB20), Wangu (GB12), Yifeng (SJ17), Lianquan (RN23), Jinjin (EX-HN12), Yuye (EX-HN12) on the affected side were needled, and the scalp acupuncture was needled.The body acupuncture, the electric acupuncture, and the scalp acupuncture were applied in combination in the acupuncture group.The acupuncture was performed once a

  10. The Influence of Respiratory Muscle Function Exercise on Clinical Symptoms and Pulmonary Functions of Con-valescent COPD Patients%呼吸肌功能锻炼对COPD康复期患者临床症状及肺功能的影响

    Institute of Scientific and Technical Information of China (English)

    毛维

    2016-01-01

    目的:探讨呼吸肌功能训练对COPD康复期患者临床症状及肺功能的影响。方法:50例COPD康复期患者进行呼吸肌功能锻炼,对比患者锻炼前后临床症状及肺功能。结果:锻炼后膈肌活动度明显高于锻炼前(P<0.05),锻炼后患者发绀情况及FVCmax、FEV1、FEV1/FVC、MMEF、PEF等肺部功能明显优于锻炼前(P<0.05)。结论:呼吸肌功能训练能明显改善COPD康复期患者的临床症状及肺功能,适合临床推广应用。%Objective:To investigate the influence of respiratory muscle function exercise on the clinical symptoms and pulmonary functions of con-valescent COPD patients. Methods:50 cases of convalescent COPD patients were all trained by respiratory muscle function exercise, and compared the situation of clinical symptoms and pulmonary functions before and after training. Results:The diaphragm activity after training was significantly higher than that of before training (P<0.05), the situation of cyanosis and pulmonary functions of FVCmax, FEV1, FEV1/FVC, MMEF, PEF were sig-nificantly better than those of before training (P<0.05). Conclusion:Respiratory muscle function exercise can significantly improve the clinical symp-toms and pulmonary functions of convalescent COPD patients, suits to clinical promotion and application.

  11. 海军飞行人员疗养前后红细胞和血红蛋白的变化及其相关性分析%Changes in red blood cells and hemoglobins of naval aviators before and after recuperation in the convalescent hospital and related analysis

    Institute of Scientific and Technical Information of China (English)

    王道建; 刘喜楠; 张东海; 李小龙

    2014-01-01

    目的:探讨海军飞行人员疗养前后红细胞和血红蛋白的变化及其相互关系。方法分析海军85名机型一、59名机型二、42名机型三飞行人员疗养前后红细胞数量、血红蛋白含量变化,并采用直线相关回归法分析其相关性。结果疗养前后海军飞行人员红细胞数、血红蛋白含量均在正常参考范围内。疗养后红细胞数下降、血红蛋白含量升高。疗养后飞行人员红细胞数与血红蛋白含量相关性更密切,机型三飞行人员红细胞数与血红蛋白含量相关性比机型一、机型二飞行人员密切。结论不同机型和飞行任务影响飞行人员红细胞数和血红蛋白含量及其相互关系,疗养可以改善飞行人员血液质量。%Objective To investigate changes in red blood cells and hemoglobin levels of naval aviators before and after recu -peration in the convalescent hospital and related analysis .Methods To analyze changes of red blood cell and hemoglobin counts in the naval aviators of type I aircraft (85 aviators), type II aircraft (59 aviators) and type III aircraft (42 aviators) before and after recupera-tion in the convalescent hospital , and their relationship was analyzed by using linear regression method .Results Red blood cell and hemoglobin counts in the naval aviators both before and after recuperation in the convalescent hospital were all within normal ranges . Red blood cell counts decreased , while hemoglobin levels elevated , after recuperation .Closer correlation between red blood cell counts and hemoglobin levels could be noticed in the aviators , following recuperation in the convalescent hospital .The correlation between red blood cell counts and hemoglobin levels in type III aviators seemed to be closer than that of the type I and type II aviators .Conclusion Different aircraft types and different flying operations had impact on red blood cell counts and hemoglobin levels , and recuperation could improve

  12. Effect of psychological intervention on the quality of life during convalescence in patients with severe burn%心理干预对重度烧伤患者康复期生活质量的影响

    Institute of Scientific and Technical Information of China (English)

    谢丽珍; 叶小兰; 杨芳

    2014-01-01

    Objective:To explore the effect of psychological intervention on the quality of life during convalescence in patients with se -vere burn.Methods:52 patients with severe burn were randomly divided into the intervention group and the control group (26 cases in each group).The patients in the control group were only given routine treatment ,nursing care and functional exercise and the extra psychological intervention was given to the patients in the intervention group .The anxiety state and the changes of quality of life of the patients were com-pared between the two groups befroe and after the intervention .Results:The scores of SDS and SAS were significantly lower after the inter-vention for one month than those before the intervention in the intervention group (P<0.05);the total score of quality of life of the patients was significantly higher in the intervention group than the control group (P<0.05).Conclusion:The psychological intervention can effec-tively alleviate the anxiety and pessimism of patients with severe burn so as to improve their quality of life .%目的:探讨心理干预对重度烧伤患者康复期生活质量的影响。方法:将52例重度烧伤患者随机分为干预组和对照组各26例,对照组仅进行常规治疗、护理及功能锻炼,干预组在此基础上实施心理干预。比较两组患者干预前后焦虑状态及生活质量变化情况。结果:1个月后,干预组SDS、SAS评分明显低于干预前(P<0.05),生活质量总分明显高于对照组(P<0.05)。结论:心理干预能有效缓解重度烧伤患者的焦虑、悲观情绪,提高患者生活质量。

  13. Caritas Convalescent Centre, Merrion Road, Dublin 4.

    LENUS (Irish Health Repository)

    O'Neill, Barry J

    2014-04-01

    Antiphospholipid syndrome and systemic erythematosus have been associated with metatarsal stress fractures. Stress fractures of the Lisfranc joint complex are uncommon injuries but have been reported to occur most frequently in ballet dancers. We present a case of an avulsion fracture of the Lisfranc joint complex that occurred spontaneously. We have reviewed the association between systemic conditions and metatarsal fractures and proposed a series of hypothetical pathological events that may have contributed to this unusual injury.

  14. Caritas Convalescent Centre, Merrion Road, Dublin 4.

    LENUS (Irish Health Repository)

    O'Neill, J L

    2011-10-01

    Appropriate nutrition in pregnancy is fundamental for maternal and fetal health, and the long-term physiological wellbeing of the offspring. We aimed to determine whether a sample of pregnant women met the national guidelines for healthy eating during pregnancy, and to examine if compliance differs when analysed by Body Mass Index (BMI) category. Subjects completed a 24-hr dietary recall, and had their BMI calculated. The mean age was 27.8 years. The mean BMI was 25.1 kg\\/m2, with 32 (31.7%) subjects overweight and 14 (13.9%) obese based on BMI category. Although the majority of subjects thought that they had a healthy diet, less than half met the recommended guidelines for each individual food group with achievement of the dairy group being particularly low. Achievement of food group recommendations was not influenced by BMI category. Public health messages on healthy eating guidelines need to be clearly communicated to pregnant women.

  15. Effects of music electrotherapy on the mental health of the commissioned and enlisted convalescent people taking part in the convoy missions%音乐电疗对改善疗养护航官兵心理健康的效果观察

    Institute of Scientific and Technical Information of China (English)

    刘继红; 矫静; 徐燕

    2014-01-01

    目的 探讨音乐电疗方法对疗养护航官兵精神状况和应激状态的改善效果.方法 将88名参加索马里护航归来在我院疗养的官兵分为2组,其中43人为实验组,45人为对照组.实验组采用我院自行研制的“康健音乐电疗仪”进行治疗,每日1次,每次30 min,10次为1个疗程.对照组只进行常规疗养.疗程前后均采用心理健康自评量表(SCL-90)、抑郁自测量表(SDS)及认知情绪调节问卷(CERQ)进行测评,重点观察精神状况和应激状态的相关指标.结果 88人均按计划完成疗养.心理测试结果显示,实验组治疗后心理健康状况显著改善,与对照组比较,SCL-90量表中除敌对、精神病性2个因子外,其余11个因子得分均显著降低(P<0.05);SDS得分显著降低(P<0.01);CERQ得分中灾难化、责备他人、满意度等3个维度得分显著改善(P<0.05).结论 音乐电疗可明显改善护航官兵的情绪和精神.%Objective To investigate the effects of music electrotherapy on the mental health and stress of the commissioned and enlisted convalescent people taking part in the convoy missions in the Gulf of Aden.Methods The health-promotion music electrotherapy instrument developed in our sanatorium was used in the treatment of those commissioned and enlisted convalescent people taking part in the convoy missions in the Gulf of Aden,once a day,30 min a session and 10 sessions a treatment course.The Self Rating Scale (SCL-90),the Self-rating Depression Scale (SDS) and the Cognitive Emotion Regulation Questionnaire (CERQ) were used to detect the above parameters both before and after treatment,with special emphasis on the observation of their mental health and stress status.Results Eighty-eight commissioned and enlisted convalescent people,among whom 43 people were in the experimental group and 45 people were in the control group,all completed their convalescent treatment profile according to schedule.Mental health detection

  16. Impact of Group Psychotherapy on Self-harmony in Convalescent Patients with Schizophrenia%团体心理治疗对恢复期精神分裂症患者自我和谐的影响

    Institute of Scientific and Technical Information of China (English)

    孙华风

    2014-01-01

    目的:对恢复期精神分裂症患者的自我和谐进行调查,并探讨团体心理治疗在其中的作用。方法60名精神分裂患者作为研究对象接受自我和谐量表( SCCA)调查,将测查结果与常模比较。同时,将研究对象随机分为团体心理治疗组30例和常规治疗组30例,比较两组患者自我和谐的差异。结果①60例精神分裂症患者SCCS中自我与经验的不和谐、自我的刻板性均高于常模,自我的灵活性低于常模,差异均具有统计学意义(t=7.97,8.96,4.28;P<0.05);②经过6周治疗后,团体心理治疗组自我与经验的不和谐和自我的刻板性降低,差异具有统计学意义(t=4.81,3.86;P<0.05)。团体心理治疗组和常规治疗组在治疗前,自我和谐性的3个维度,即自我与经验的不和谐、自我的灵活性、自我的刻板性经比较差异无统计学意义(t=0.82,0.54,0.34;P>0.05)。治疗后,团体心理治疗组的自我与经验的不和谐和自我的刻板性均低于常规组,自我的灵活性高于常规治疗组,差异具有统计学意义(t=-2.31,-3.26,4.08;P<0.05)。结论精神分裂症患者自我和谐性差,团体心理治疗可以缓解患者的交往焦虑,提高自我和谐性。%Objective To investigate self-harmony in convalescent patients with schizophrenia and explore the role of group psycho-therapy.Methods 60 schizophrenic patients as research subjects completed Self Consistency and Congruence Scale(SCCS),and the survey findings were compared with the norm.Meanwhile,the subjects were randomly divided into group psychotherapy group 30 cases and conventional treatment group 30 cases,and the differences of self-harmony in two groups were compared.Results ①Self-experi-ence disharmony,self-stereotypes of SCCS in 60 patients with schizophrenia were higher than the norm,self-flexibility lower than the norm

  17. 精神分裂症恢复期患者社会功能与精神症状的关系及自尊与自我效能的中介效应%The relationship between social functioning and psychiatric symptoms with schizophrenia of the convalescent stage: Mediating roles of self-esteem and self-efficacy

    Institute of Scientific and Technical Information of China (English)

    焦亚辉; 王丽娜; 周郁秋; 李刚

    2013-01-01

    目的 探讨精神分裂症恢复期患者社会功能与精神症状的关系,以及自尊、自我效能在社会功能与精神症状间的中介效应.方法 采用个人和社会功能量表(PSP)、简明精神病性量表(BPRS)、自尊量表(SES)及一般自我效能感量表(GSES),对门诊复查的精神分裂症恢复期患者进行调查.结果 精神分裂症恢复期患者的社会功能可直接预测其精神症状,自尊及自我效能均可直接负向预测其精神症状;自尊和自我效能在恢复期患者社会功能与精神症状间起着部分中介效应.结论 精神卫生工作人员对精神分裂症恢复期患者进行社会功能训练时,应同时重视自尊及自我效能的中介作用,在进行全面评估的基础上,给予针对性的心理社会干预措施,从而有效改善患者的精神症状.%Objective To examine the relationship between social functioning and psychiatric symptoms,and the mediation effect of self-esteem and emotion between social functioning and psychiatric symptoms among schizophrenia patients of the convalescent stage.Methods The Personal and Social Performance Scale,Brief Psychiatric Rating Scale,Self-Esteem Scale,General Self-Efficacy Scale were used to investigate patients with schizophrenia of the convalescent stage of Chifeng.Results Social functioning for patients with schizophrenia of the convalescent stage could predict psychiatric symptoms directly.Self-esteem and self-efficacy could make a negative prediction for psychiatric symptoms.Self-esteem and self-efficacy played a mediating role between social functioning and psychiatric symptoms.Conclusions Mental health workers should pay close attention to the mediating roles of self-esteem and self-efficacy,and give comprehensive assessment for the patients and provide continuous individualized psychosocial interventions,and thereby to alleviate psychiatric symptoms.

  18. 老年抑郁症恢复期患者的应对方式及社会支持的评价%Coping style and social support in senile depressive patients at convalescent stage

    Institute of Scientific and Technical Information of China (English)

    李凌; 程淑英; 李占江; 李建明; 徐应军

    2006-01-01

    利用度低于对照组(P<0.05),两组受试对象客观支持分差异无显著性.抑郁组患者积极应对与社会支持总分、主观支持和对支持的利用度呈正相关(P<0.01),消极应对与社会支持总分、主观支持和对支持的利用度呈负相关(P<0.01).结论:社会支持中的主观支持和对支持的利用度以及消极的应对方式与老年抑郁症发病有关.%BACKGROUND: The clinical manifestation of senile depression is not typical. The patients with senile depression seldom visit in general hospital, which is hard for clinical doctors to identify the disease at early time.OBJECTIVE: To research the traits of Coping style and social support in the recovered senile depressive patients.DESIGN: Case-control observation.SETTING: Department of Psychology in North China Coal Medical College.PARTICIPANTS: Ninety-six senile depressive patients at convalescent stage were selected for depressive group in clinic service of Beijing Anding Hospital from November 2004 to October 2005. Inclusion criteria: ①Aged≥60 years old. ② Coded with depression with DSM-Ⅳ. ③ Being the period of recovery, geriatric depression scale(GDS)≤10. ④Informed consent was obtained from each participant. Exclusion criteria: ① Depression resulted from other factors for instance substance abuse and schizophrenia,etc. ②Serious and instable body disease such as cerebrovascular disease,liver and kidney disease, falling sickness, disease of internal secretion. Totally 104 questionnaires were sent out, and 96 were qualified. 37 were male and 59 were female in the depressive group, aged 60-87 years old,with average of (68±6)years. The course of disease was 0.3-11 years, with mean of 2 years. The elderly from ordinary community of Beijing were invited for control group. Inclusion criteria: ①Aged ≥ 60 years old; ② No mental disease. ③ Informed consent was obtained from each participant.Exclusion criteria: The same to the depressive group. There are 100 participants

  19. Correlation between seff-efficacy and self-management behaviors in convalescent patients with acute pancreatits%急性胰腺炎康复期患者自我效能与自我管理行为的相关性

    Institute of Scientific and Technical Information of China (English)

    钱雅芬; 许方蕾; 张圆圆

    2011-01-01

    目的 探讨急性胰腺炎康复期患者自我效能与自我管理行为之间的相关性.方法 选取急性胰腺炎康复期患者70例为研究对象,采用自我效能量表及自我管理行为调查问卷结合的方法对其调查,统计患者自我管理水平与自我效能水平的评分并分析其相关性.结果 急性胰腺炎康复期患者自我管理水平得分为(14.91 ±3.21)分,85.7%的患者自我管理水平高,但其中运动锻炼水平较高者只占40%,且不良生活习惯管理水平中差者占34.2%;患者自我效能水平得分为(15.23±3.53)分,37.1%的患者自我效能高,48.6%较高,14.3%中等,无自我效能低者;急性胰腺炎康复期患者自我效能与自我管理呈正相关(r=0.803,P<0.01),各类自我效能与相应的自我管理也均呈正相关(r=0.235~0.843,P<0.05).结论 急性胰腺炎康复期患者需加强有关运动锻炼及不良生活习惯的健康教育,科学运用自我效能理论,减少急性胰腺炎康复期患者的复发、提高生活质量.%Objective To study the relationship between self-efficacy and self-management behaviors in convalescent patients with acute pancreatitis.Methods Totals of 70 convalescent patients with acute pancreatitis were investegeted with self-efficacy scale and self-management behaviors questionnaire,then collected score respectively,analyzed the relation between self-efficacy and self-management behaviors.Results The score of selfmanagement questionnaire was( 14.91 ± 3.21 ),85.7% patients had a high management level,and only 40%patients of them had a high level of exercise,34.2% patients had a low level of bad living life management.The score of self-efficacy scale was( 15.23 ±3.53),and 37.1% patients had a high self-efficacy level,48.6% patients had a higher level,14.3% patients had a medium level,no low self-efficacy was found.Self-efficacy in convalescent patients with acute pancreatitis was positively correlated with

  20. 精神分裂症恢复期患者病耻感对生活质量的影响:社会支持及应对方式的中介效应%The effect of stigma on the quality of life among convalescent schizophrenia patients:the mediating effect of social support and coping style

    Institute of Scientific and Technical Information of China (English)

    宇虹; 王丽娜; 周郁秋; 李国华; 袁瑷琳; 孙玉静

    2015-01-01

    Objective:To examine the relationship between stigma and the quality of Life among convalescent schizophrenia patients, and to explore the mediating effect of social support and coping style between stigma and the quality of life. Methods:The perceived Devaluation-Discrimination Scale, Simplified Coping Style Questionnaire, Social Support Rating Scale, and the Schizophrenia Quality of Life Scale were used to investigate the convalescent schizophrenia patients. Results:Structural equation model ift index:χ2/df=1.673, GFI=0.950, AGFI=0.903, RMSEA=0.061. Stigma, social support and coping style among convalescent schizophrenia patients could predict the quality of life directly. Social support and coping style played a mediating role between stigma and the quality of life. Conclusions:Mental professionals should pay more attention to stigma effect on quality of life among convalescent schizophrenia patients in their clinical practice. Based on summarizing the different paths, we should improve the quality of life of the patients. by path-based intervention.%目的:探讨精神分裂症恢复期患者病耻感与生活质量的关系,以及社会支持、应对方式在病耻感与生活质量间的中介效应。方法:采用贬低-歧视感知量表、社会支持量表、简易应对方式问卷及精神分裂症患者生活质量量表,对在门诊复查的183例精神分裂症恢复期患者进行调查。结果:结构方程模型拟合指数χ2/df=1.673,GFI=0.950,AGFI=0.903, RMSEA=0.061;精神分裂症恢复期患者的病耻感、社会支持、积极应对及消极应对均可以直接预测其生活质量,社会支持、积极应对和消极应对在患者的病耻感与生活质量间起着部分中介效应。结论:精神卫生人员在提高精神分裂症恢复期患者生活质量实践工作中,应重视病耻感对患者病情的影响,在总结不同作用路径的基础上,开展以路径为依托的干预,提高患者的生活质量。

  1. The gradient heat stress impact on the convalescent core body temperature and prognosis in heatstroke rats%梯度热应激对大鼠核心体温调节及预后的影响

    Institute of Scientific and Technical Information of China (English)

    付炜; 刘亚楠; 耿焱; 彭娜; 雷玉梅; 苏磊; 唐忠志

    2014-01-01

    the survival within 72 h was also recorded.Results With varying degrees of heat stress , the Tc of rats was presented in different patterns .Biphasic rise was observed in 41℃and 42℃groups, while triphasic rise was observed in 43℃and 44℃groups.Out of heat stress, the Tc in 41℃and 42℃groups was returned to normal levels within 1 h, while low Tc was observed in 43℃and 44℃groups, and especially in 44℃group.Deaths were recorded in 43℃and 44℃groups, while significantly worse prognosis was re-vealed in 44℃group according to survival analysis (P<0.01).Conclusion Different degrees of heat stress have signi-ficant effect on Tc in rats.The higher degree of heat stress , the lower Tc is presented during the convalescence , suggesting worse prognosis .

  2. 特勤疗养营养管理系统对特勤疗养员膳食营养干预的效果分析%Effect analysis of the special convalescence nutrition management system on the dietary intervention of the special recuperator

    Institute of Scientific and Technical Information of China (English)

    董晓敏; 蔡缨; 武彩莲

    2014-01-01

    Objective The special convalescence nutrition management system was applied for dietary intervention on special convalescence recuperator ,so as to establish an effective special mode for the management of convalescence nutrition .Methods One hundred and eighty special recuperators were extracted from Hangzhou Sea Sanatorium of Nanjing Military Region by the method of clus -ter sampling to survey the dietary problems ,through the application of the special convalescence nutrition management system ,again to choose a certain sample size ,then the nutritional status of the secret service recuperator were compared .Results Compared with non-interfered persons,in addition to the fruit,the residual rate of all kinds of food such as grain ,meat,poultry,eggs,fish,milk,sugar,vege-table oil,vegetables,dried vegetables were decreased obviously (P<0.05) than before intervention;the unreasonable dietary structure were improved obviously ,and the supply of three large energy-yielding nutrients were conformed to the military standard .The intake of cooking oil was 65.1 g/d,cholesterol was 792.4 mg/d,basically meet the military standard;in addition to vitamin A,the intake of the rest nutrients were standard or exceed the standard ,including vitamin A,vitamin B1,vitamin B2,vitamin C and calcium.Conclusion The application of special convalescence nutrition management system can effectively improve the catering level of dietitians ,reduce the residual rate of food ,and improve the dietary behaviors and nutritional status of the special recuperator .%目的:应用“特勤疗养营养管理系统”对特勤疗养员进行膳食营养干预,以期建立有效的特勤疗养膳食营养管理模式。方法按整群抽样方法抽取南京军区杭州疗养院海勤疗养区180名特勤疗养员进行膳食调查,了解当前特勤疗养员存在的膳食营养问题,应用“特勤疗养营养管理系统”进行膳食营养干预,再次选择一定的样本量进行膳

  3. 康复期精神分裂症患者社会功能与家庭亲密度和适应性的相关研究%Study on correlation between social function and family adaptability and cohesion of convalescent schizophrenic patients

    Institute of Scientific and Technical Information of China (English)

    龙斌; 童颜; 毕燕君

    2015-01-01

    Objective To explore correlation between social function with family cohesion and adaptability in convalescent schiz-ophrenic patients. Methods 100 convalescent schizophrenic patients after treatment in Zigong Mental Health Center were selected. They were investigated with Chinese Vision for Family Adaptability and Cohesion Scale(FACES II - CV)and Morningside Rehabilita-tion Status Scale(MRSS). Results The scores of ideal cohesion had a negative correlation with the scores of social scale( r =- 0. 525,P < 0. 05). The scores of real cohesion and real adaptability had a negative correlation with the scores of lack of activity abil-ity scale,dependency scale,social scale and MRSS(r = - 0. 198,- 0. 078,- 0. 372,- 0. 225,P < 0. 01;r = - 0. 092,- 0. 043,- 0. 204,- 0. 105,P < 0. 01). The scores of dissatisfactory cohesion had a positive correlation with the scores of dependency scale, lack of activity ability scale and MRSS(r = - 0. 531,- 0. 401,- 0. 395,P < 0. 05 or 0. 01). The scores of dissatisfactory adaptability had a positive correlation with the scores of dependency scale(r = 0. 402,P < 0. 01). Conclusion We should strengthen the family intervention in convalescent schizophrenia patients,and guide their family adaptability and cohesion consciously to improve their social function.%目的:探讨康复期精神分裂症患者的社会功能与家庭亲密度和适应性的相关性。方法选取在自贡市精神卫生中心住院治疗后出院的康复期精神分裂症患者100例为研究对象。采用家庭亲密度与适应性量表中文版(FACESⅡ- CV)和 Morningside 康复状态量表(MRSS)对患者进行评定。结果理想亲密度评分与社交量表评分呈负相关(r =-0.525,P <0.05);实际适应性及实际亲密度评分分别与活动能力缺乏量表、依赖量表、社交量表评分及 MRSS 总评分呈负相关(r =-0.198,-0.078,-0.372,-0.225,P <0.01;r =-0.092,-0.043,-0.204,-0.105,P <0.01);不满

  4. 川崎病恢复期颈动脉内膜-中膜厚度变化及相关因素分析%Study of carotid intima media thickness and its correlated factors in children at the convalescent phase of Kawasaki disease

    Institute of Scientific and Technical Information of China (English)

    牛峰海; 王瑜; 蔡文仙; 范国贞; 任雪云

    2012-01-01

    Objective To observe the change of carotid intima-media thickness and analysis of its correlated factors at the convalescent phase in children with Kawasaki disease, and to provide a scientific basis for prevention and treatment of vascular lesions. Methods A cohort of 60 children during the age of 2 to 4 years old was studied, which comprised 30 children at the convalescence phase of Kawasaki disease (KD group) and 30 healthy age-matched children (control group). Carotid arterial intima-media thickness (IMT) , malondialdehyde ( MDA) , interleukin-1 beta (IL-1|3) , interferon-gamma (IFN-7) and body mass index (BMI) were evaluated. Results IMT in KD group and control group was (0.40 ± 0.03) mm and (0.37 ± 0.04) mm respectively. IMT in KD group was higher than that in control group (P 0.05). IMT was positively correlated with the plasma level of MDA (r = 0.463, P < 0.05), but not significantly correlated with the levels of IL-iβ, IFN-7 and BMI. Conclusions The increased carotid IMT in children at the convalescent phase of Kawasaki disease is associated with oxidative stress.%目的 观察川崎病(KD)患儿恢复期颈动脉内膜-中膜厚度变化及相关因素,为防治KD血管病变提供科学依据.方法 收集30例2 ~ 4岁KD恢复期患儿及30例同年龄健康儿童,测定其颈动脉内膜-中膜厚度(IMT)、丙二醛(MDA)、白介素-1β(IL-1β)、γ-干扰素(IFN-γ)和体质指数(BMI).结果 KD患儿颈动脉IMT为(0.40 ± 0.03)mm,对照组为(0.37 ± 0.04)mm,两者比较差异有统计学意义.KD患儿MDA 为(2.56±0.18)nmol/ml,对照组为(2.09±0.24)nmol/ml,两者比较差异有统计学意义.两组间IL-1β、IFN-γ、BMI比较,差异均无统计学意义.直线相关分析显示,颈动脉IMT与MDA呈正相关(r = 0.463,P < 0.05),而与IL-1β、IFN-γ和 BMI水平均无相关性(P均> 0.05).结论 KD恢复期患儿颈动脉内膜-中膜增厚,与氧化应激有关.

  5. 单侧初次人工髋关节置换患者康复期下肢关节的运动状况研究%Study of lower limbs joints movement of patients with unilateral and primary artificial hip replacement in con-valescence

    Institute of Scientific and Technical Information of China (English)

    龙术民; 杨陈诚; 杜勇

    2015-01-01

    Objective To explore the lower limbs joints movement of patients with unilateral and primary artificial hip replacement in convalescence. Methods 100 cases of patients with unilateral and primary artificial hip replacement in convalescence were chosen as the ob-servation group,while 100 cases of matched healthy subjects were included as the control groups. The three-dimensional kinematics and mo-tion of lower extremities were monitored. Results The difference in operated knee flexion,operated ankle planter flexion and operated hip flexion of the observation group and the observation group were statistically significant(P<0. 05). The difference in the joint angle peak of hip backward extension of sagittal section,hip adduction of frontal plane,knee adduction of frontal plane,ankle adduction and outreach of frontal plane,hip internal rotation and extorsion of horizontal plane,ankle rotation and extorsion of horizontal plane in two groups were statisti-cally significant(P<0. 05). Conclusion There are abnormal prosthetic joint movement,limited range of operated movement in patients with unilateral and primary artificial hip replacement in convalescence,and coterminous kinematic chains appear compensatory changes,it should strengthen the muscle exercises of operated hip.%目的:探究单侧初次人工髋关节置换康复期患者下肢关节的运动状况。方法选择100例单侧初次人工髋关节置换康复期患者作为观察组,纳入100例相匹配的健康体检者作为对照组,均进行下肢三维运动学与动力学监测。结果观察组患者术侧膝关节、踝关节、髋关节屈曲力矩峰值与对照组右下肢相比,差异有统计学意义(P<0.05)。观察组患者术侧矢状面髋后伸、额状面髋内收、额状面膝内收、额状面踝内收和外展、水平面髋内旋和外旋、水平面踝内旋和外旋等关节角度峰值分别与对照组右下肢相比,差异有统计学意义(P<0.05)。结论单侧初

  6. 人文关怀对康复期精神病患者心理健康状况及治疗依从性的影响%Influences of humanistic care on mental health status and treatment compliance of convalescence psychosis patient

    Institute of Scientific and Technical Information of China (English)

    王雪娣

    2013-01-01

    目的探讨人文关怀对康复期精神病患者的心理健康状况及治疗依从性的影响。方法将200例康复期精神分裂症患者随机分为两组,每组100例,两组患者均予以常规治疗及护理管理,在此基础上研究组接受人文关怀护理管理,观察8周。于人文关怀护理8周末采用症状自评量表评定两组的心理健康状况,同时评定两组的住院满意度及治疗依从性。结果人文关怀护理干预8周末研究组症状自评量表各因子评分均显著低于对照组(P<0.01);住院满意度及治疗依从性均显著高于对照组(P<0.01)。结论人文关怀护理能显著提高康复期精神病患者的心理健康水平、住院满意度及治疗依从性,有利于促进患者的全面康复。%Objective To explore the influences of humanistic care on mental health status and treatment compliance of convalescence patients with psychoses .Methods A total of 200 convalescence schizophren-ics were randomly assigned to two groups of 100 ones each ,both groups received routine treatment and nursing ,and research group was plus humanistic care for 8 weeks .Mental health conditions were assessed with the Symptom Checklist-90 (SCL-90) at the end of the 8th week ,so were degree of satisfaction and treatment compliance did .Results Each factor score of the SCL-90 were significantly lower (P<0 .01) and degree of satisfaction and treatment compliance higher (P<0 .01) in research than control group at the end of the 8th week (P<0 .01) .Conclusion Humanistic care could notably improve mental health level , degree of satisfaction and treatment compliance and is beneficial to patients’ comprehensive rehabilitation .

  7. 1H-MRS study of the auditory cortex in patients with severe sudden sensorineural hearing loss in acute and convalescent stages%重度突发性聋患者急性期与恢复期听皮层磁共振波谱分析

    Institute of Scientific and Technical Information of China (English)

    窦晓清; 陈贤明; 梁永辉; 张丽卫; 罗碧强; 邓意红

    2012-01-01

    目的 利用氢质子磁共振波谱(1H-MRS)技术,研究单侧重度突发性聋患者急性期与恢复过程中听皮层的代谢变化.方法 选取右耳重度突发性聋急性期患者15例(发病时间<1周),经过常规治疗3个月后恢复期患者9例,健康志愿者10例作为对照组.将全部受试者行磁共振波谱检测,测定双侧颞横回N-乙酰天门冬氨酸(NAA)、肌酸(cr)、胆碱(Cho)、γ-氨基丁酸(GABA)、谷氨酰胺及谷氨酸复合物(Glu)的峰下面积并计算NAA/Cr、Cho/Cr、GABA/Cr、Glu/Cr的比值.分析重度突聋急性期与3个月后双侧听皮层代谢差异.结果 与对照组相比,突聋急性期双侧NAA/Cr、双侧Cho/Cr,右侧GABA/Cr、右侧Glu/Cr比值无明显差异(P>0.05),而左侧听皮层GABA/Cr比值明显下降,Glu/Cr明显上升(P<0.05).突聋3个月组双侧NAA/Cr、右侧Cho/Cr、右侧GABA/Cr、右侧Glu/Cr比值无明显差异(P>0.05).左侧Cho/Cr比值稍下降,GABA/Cr比值略上升但仍低于对照组,Glu/Cr比值略下降但仍高于对照组(P<0.05).结论 磁共振波谱可以为人体活体听皮层代谢物浓度改变提供有价值的信息;突聋患者对侧听皮层GABA、G1u代谢异常,提示听觉中枢可能发生代谢改变、功能重组.%Objective To study the metabolic changes of the auditory cortex in the patients with unilateral severe sudden sensorineural hearing loss (SSNHL) in acute and convalescent stages by using proton magnetic resonance spec-(roscopy (1H-MRS). Methods 15 patients with acute severe SSNHL in right ear were studied (the onset time of severe sudden deafness 0.05). The GABA/Cr ratio was significantly lower in the left auditory cortex of severe SSNHL patients in the acute stage than that in the control group( P 0. 05). The Cho/Cr ratio was significantly lower in the left auditory cortex of severe SSNHL patients in the convalescent stage than that in the control group(P<0.05). The GABA/Cr ratio was slightly increased but still significantly

  8. "烧山火、透天凉"补泻跷脉法对缺血性中风恢复期患者下肢痉挛的影响研究%Effect of Acupuncture in Qiao Meridians of Shaoshanhuo and Toutianliang to Lower Limb Spasm of Ischemic Stroke Patients at Convalescence

    Institute of Scientific and Technical Information of China (English)

    李旗; 田福玲

    2013-01-01

    Objective To investigate the effect of acupuncture in Qiao meridians of the traditional Shaoshanhuo and Toutianliang to lower limb spasm of the in patients with ischemic stroke at the convalescent stage. Methods Totally 100 patients were according to the random number table divided as trial group and control group, with 50 in each. The patients in the control group were treated with the conventional body acupuncture therapy while those in the trial group with acupuncture in Qiao meridians of the traditional Shaoshanhuo and Toutianliang. For both groups the treatment course consisted of 28 days. Ashworth scale was adopted to measure muscular tension changes of the lower limb 0. 5 h before the initial treatment, and 0. 5 h, 1h, 2h, 4h, 8 h and 28d after the treatment. Results The Ashworth scores 0. 5 h after the initial treatment in the two groups [ ( 1. 84 ± 1. 04 ) vs. ( 2. 08 ± 0. 97 ) ] showed a significant difference ( P < 0. 05 ) . And so did the Ashworth scores 28 day after the treatment [ ( 1. 43 ±0. 96 ) vs. ( 1. 85 ±0. 96 ) ] ( P <0. 05 ) . There was also a statistically significant difference between the two groups in improvement degree of lower limb spasm ( P = 0. 022 ) . Conclusion The acupuncture in Qiao meridians of the traditional Shaoshanhuo and Toutianliang is of both recent and late effect to reduce muscular spasm for patients with ischemic stroke at convalescence.%目的 探讨"烧山火、透天凉"补泻跷脉法对缺血性中风恢复期患者下肢痉挛的影响.方法 将100例患者按随机数字表法随机分为试验组与对照组,各 50例.试验组采用"烧山火、透天凉"补泻跷脉法,即泻阴补阳跷脉法治疗,对照组采用传统体针法治疗,两组均治疗28 d.应用 Ashworth 量表分别于首次治疗前 0.5 h、治疗后0.5 h、1 h、2 h、4 h、8 h 和28 d测定患者的下肢肌痉挛并观察疗效.结果 两组首次治疗后0.5 h Ashworth评分[(1.84±1.04)分 vs.(2.08±0.97)分]比

  9. 穴位注射对缺氧缺血性脑病患儿恢复期肌张力低下的疗效评价%The effect of point injection on convalescence hypomyotonia of hypoxic ischemic encephalopathy children

    Institute of Scientific and Technical Information of China (English)

    付惠玲; 陈惠军; 田金富

    2016-01-01

    目的:探讨穴位注射对缺氧缺血性脑病恢复期肌张力低下患儿的治疗效果。方法将96例缺氧缺血性脑病恢复期肌张力低下患儿采用随机数字表法分为治疗组46例,对照组50例,均予以常规综合治疗,治疗组在此基础上加用单唾液酸四己糖神经节苷脂注射液20 m g穴位注射,对照组加用单唾液酸四己糖神经节苷脂注射液20 m g静脉滴注,观察3个月。治疗后采用徒手肌力法评定肌力,表面肌电评估系统评估肱二头肌的恢复情况。结果治疗3个月后两组患儿肱二头肌徒手肌力检查结果显著优于治疗前(P<0.01),且治疗组显著优于对照组(P<0.05),肌电信号的肌电积分值及均方根值均显著高于治疗前(P<0.01),且治疗组显著优于对照组(P<0.01)。结论在综合康复治疗的基础上辅以局部穴位注射,有助于进一步改善患儿的肌力及肱二头肌的恢复状况。%Objective To explore the therapeutic effect of point injection on convalescence hypomyotonia of hypoxic ischemic encephalopathy (HIE) children .Methods Ninety‐six HIE children with convalescence hypomyotonia were randomly assigned to treatment (n=46) and control group (n=50) ,both groups re‐ceived routine combined therapy ,on this basis treatment group was plus point injection of monosialotetera‐hexosyl ganglioside 20 mg ,and control group plus monosialoteterahexosyl ganglioside 20 mg iv gtt for 3 months .After treatment muscle strengths were assessed with Manual Muscle Testing (MMT) and recov‐ery states of biceps brachii with surface myoelectricity evaluation system .Results After 3 month treat‐ment the MMT results of biceps brachii of both groups were better compared with pretreatment (P<0 .01) ,so was that in treatment than control group (P<0 .01) ,scores and root mean square values of elec‐tromyographic signal of both groups were significantly higher compared with

  10. 内观疗法对社区精神分裂症康复期患者疗效对照研究%A randomized controlled study of NaiKan therapy efficacy on the convalescent schizophrenic patients in the community

    Institute of Scientific and Technical Information of China (English)

    高红艳; 班春霞; 赵姣文; 朱敏珏; 谭大勇; 吴会敏

    2015-01-01

    目的:探讨内观疗法对社区精神分裂症康复期患者的临床疗效。方法将112例社区精神分裂症康复期患者随机分为内观治疗组52例和对照组56例,进行9周治疗,对照组采用药物治疗,内观治疗组在此基础上联合内观治疗。在治疗前、治疗后进行阳性与阴性症状量表(PANSS),症状自评量表(SCL‐90)中人际关系敏感因子和敌对因子、自知力与治疗态度问卷(ITAQ)测定。结果治疗前,2组在 PANSS总分、阳性症状量表分、阴性症状量表分、一般精神病理量表,SCL‐90人际关系敏感因子、敌对因子,ITAQ量表评分,差异均无统计学意义(P>0.05)。治疗后,2组患者在PANSS量表总分、阴性症状量表、一般精神病理量表,SCL‐90人际关系敏感因子评分及敌对因子评分上,差异均具有统计学意义(P<0.05);在 PANSS阳性症状量表分、ITAQ量表评分上,差异均无统计学意义(P>0.05)。结论内观治疗有助于改善社区精神分裂症康复期患者的精神症状及人际关系,适合在社区中开展。%Objective To investigate the efficacy of Naikan therapy in patients with community convalescent schizophrenia .Methods The 112 community convalescent schizophrenic patients were randomly divided into Nai‐kan therapy group ( n=52) and control group ( n=56) ,and were treated with antipsychotic agent therapy com‐bined with Naikan therapy and antipsychotic agent therapy for 9 weeks ,respectively .Efficacy at baseline ,and the end of 9th week after the treatment were assessed respectively by positive and negative syndrome scale(PANSS) , interpersonal sensitivity factor and hostile factor of SCL‐90 of symptom checklist 90(SCL‐90)and insight and treat‐ment attitudes questionnaires (ITAQ) .Results At the baseline ,there was no significant difference in PANSS to‐tal score ,negative symptom score ,positive symptom score ,general

  11. Clinical research of treated withparanoia schizophrenia patients at convalescence by discontiguous Naikan cognitive therapy%分散内观认知疗法治疗偏执型精神分裂症康复期患者的临床研究

    Institute of Scientific and Technical Information of China (English)

    秦进芳; 陈丽曼; 杨红; 高兴; 陈文峰

    2015-01-01

    Objective To observe the effect of Discontiguous NaiKan Cognitive Therapy (DNCT) on patients with paranoia schizophrenia during their convalescence so as to offer better guidance on clinical practice.Methods In this study,72 patients met the inclusive standard were included and randomly divided into two groups,the combined Olanzapine and DNCT treatment group (the study group) and the Olanzapine alone treatment group (the control group),and the improvement of their symptoms,cognitive function and activity of daily living during treatment as well as their life quality at the Year 1 follow-up were subject to comparison.Results After treatment,compared with the control group,the study group had lower factors except hostility,terror,crankiness and psychosis factors,and its MMSE,ADL,role function and emotional function scores were markedly increased,the differences being statistically significant (P<0.05).Conclusion The application of combined DNCT and medication to patients with paranoia schizophrenia during their convalescence may effectively improve their clinical symptoms,cognitive function,activity of daily living and life quality.%目的 探讨在偏执型精神分裂症康复期患者中应用分散内观认知疗法的效果.方法 将符合病例纳入标准的72例患者纳入研究,随机分为奥氮平结合分散内观认知疗法的治疗组(简称研究组),以及单纯奥氮平的治疗组(简称对照组),观察两组患者治疗期间的症状、认知功能、日常生活能力改善情况,以及随访1年的生活质量比较.结果 治疗后,与对照组比较,除敌对因子、恐怖因子、偏执因子、精神病因子外,研究组的其余各因子均降低明显,MMSE评分、ADL评分、角色功能、情感功能得分均升高明显,差异具统计学意义(P<0.05).结论 对于偏执型精神分裂症康复期患者应用分散内观认知疗法结合药物治疗可有效改善其临床症状及认知功能,提高日常生活能力及生活质量.

  12. 康复教育对急性心肌梗死恢复期患者生活质量的影响%Effect of rehabilitation education on the quality of life of the patients in the convalescence stage of the acute myocardial infarction

    Institute of Scientific and Technical Information of China (English)

    王彦; 王爱平; 贾连春; 吴丽平; 姚景鹏

    2005-01-01

    BACKGROUND: There is still recrudescent risk in the convalescence stage of the acute myocardial infarction and the living condition is relatively poor. The study proved that it has relation to that the patients haven't mastered the scientific conformable life style for the myocardial infarction recovery.OBJECTIVE: Take the rehabilitation education to help the patients set up conformable life style so as to improve the quality of life.DESIGN: The patients in the convalescence stage of the acute myocardial infarction were chosen for the control study.SETTING: Nursing Institute of Beijing Medical University and two university hospitals.PARTICIPANTS: The cases were taken from the Cardiology Departm ents of the Second and Third Affiliated Hospital of Beijing Medical University from April 1999 to November 1999. Inclusive standard: the first onset of the acute myocardial infarction and voluntary for this study. Exclusive standard: not the first onset of the acute myocardial infarction. The control group contained 35 patients hospitalized from April 1999 to June 1999 conforming to the including standard. The experimental group contained 40 patients hospitalized from August 1999 to November 1999 conforming to the including standard. After eliminating the special unmatched cases and missed follow-up cases in the experimental group, the final 30 cases were respectively involved in the both groups. There were 23 male cases and 7 female cases in the control group with the mean age of(60 ± 14), and 22 male cases and 8 female cases in the experimental group with the mean age of(62 ± 10) . There was no significant difference in the age and sex in these two groups.METHODS: The Neuman health care system pattern was taken as the theoretical frame for the rehabilitation education of the patients in the convalescent stage of the acute myocardial infarction. That is the conver genceteaching and personal illustration accompanied the rehabilitation education hand books were adopted for

  13. Observation of Shanzhajiangzhi pill on the treatment of phlegm-stasis blocking collateral type cerebral in-farction convalescence with hyperlipidemia%山楂降脂丸治疗痰瘀阻络型脑梗死恢复期合并高脂血症90例临床观察

    Institute of Scientific and Technical Information of China (English)

    孔令凤; 刘继东; 赵军旗; 王继华; 王素丽; 孟雪莲

    2014-01-01

    目的:观察山楂降脂丸治疗痰瘀阻络型脑梗死恢复期合并高脂血症的临床疗效。方法将180例痰瘀阻络型脑梗死恢复期合并高脂血症患者随机分为2组。对照组90例单纯采用西医方法治疗,即抗血小板聚集、降脂、稳定斑块、调控血糖、降压及改善心肌供血等治疗。治疗组90例在对照组治疗基础上加用山楂降脂丸治疗。2组均治疗6周,观察2组治疗前后胆固醇( TC)、甘油三酯( TG)、低密度脂蛋白胆固醇( LDL-L)水平变化情况及治疗前后全血黏度(高切、低切)、血浆黏度及红细胞刚性指数的变化情况。结果2组总有效率比较差异有统计学意义(P<0.05),治疗组优于对照组;2组治疗后TC、TG、LDL-L、全血黏度(高切、低切)、血浆黏度及红细胞刚性指数均降低(P<0.05),且治疗组下降程度优于对照组(P<0.05)。结论山楂降脂丸能改善痰瘀阻络型脑梗死恢复期的临床症状、血脂水平及血液流变学指标,疗效确切。%Objective To observe the effect of Shanzhajiangzhi pill on the treatment of phlegm-stasis bloc-king collateral type cerebral infarction convalescence with hyperlipidemia.Methods 180 cases of phlegm-stasis blocking Collateral type cerebral infarction convalescence with hyperlipidemia were randomly divided into two groups. 90 cases in control group were treated by simple western medicine therapy, including Antiplatelet aggregation and lip-id, stable plaques, control blood sugar, depressurization, improving myocardial blood supply.90 patients in treat-ment group were treated by Shanzhajiangzhi pill on the basis of control group treatment.The course was six weeks in two groups.The changes of TC, TG and LDL-C levels before and after treatment were observed in two groups.The changes of whole blood viscosity ( high shear, low shear) , plasma viscosity and erythrocyte rigidity index before and after

  14. 脑卒中恢复期下尿路功能障碍相关、预测因素及尿流动力学%The risk factors of lower urinary tract dysfunction and urodynamics study in stroke patients in the convalescent stage

    Institute of Scientific and Technical Information of China (English)

    芦海涛; 张通

    2010-01-01

    目的 观察脑卒中恢复期下尿路功能障碍的发生情况、相关因素和预测因素以及尿流动力学特点.方法 脑卒中恢复期患者131例,均收集一般资料、神经系统相关专科情况并详细了解排尿情况及既往病史,判断患者有无下尿路功能障碍及其类型,进行相关分析及尿流动力学检查.结果 脑卒中后下尿路功能障碍发生率为44.3%,症状由多到少依次为:尿失禁、尿频、尿急、夜尿增多、排尿等待及排尿费力、尿潴留.MMSE量表评价认知功能有障碍组较无障碍组下尿路功能障碍发生率高1.8倍(χ~2=18.683,P=0.000);改良巴氏指数≤20分者较≥25分者下尿路功能障碍发生率高1.2倍(χ~2=16.832,P=0.000).经Logistic回归分析发现年龄(OR=1.622,95%CI 1.183~2.224)、急性期留置导尿时间(OR=3.236,95%CI 1.335~7.840)及病灶单或多发(OR=1.296,95%CI 1.083~1.550)对脑卒中恢复期下尿路功能障碍发生有预测价值.尿流动力学检查发现78.9%患者逼尿肌过度活动,10.5%逼尿肌活动正常,10.5%逼尿肌无反射,无明确逼尿肌-括约肌协同失调患者.结论 脑卒中恢复期下尿路功能障碍较为常见,以储尿期排尿障碍为主.下尿路功能障碍的患者高级脑功能及日常生活能力低下.高龄、持续留置导尿及多发病灶对恢复期下尿路功能障碍有一定的预测价值.尿流动力学改变多为逼尿肌过度活动,但症状与尿流动力学表现不完全一致,必要时应做此检查以利于进一步治疗.%Objective To analyze the prevalence of lower urinary tract dysfunction in stroke patients in the convalescent stage,its risk factors and the characteristics of urodynamics.Methods One hundred and thirty-one stroke patients in the convalescent stage were included in this study.Their general information,neurological system reviewing and past history were obtained,especially detailed micturitional histories.In patients with urinary symptoms

  15. Effect of muscle strength exercise on surface electromyography over thigh in stroke patients with hemiplegia during convalescence%肌力训练对脑卒中恢复期偏瘫患者大腿表面肌电的影响

    Institute of Scientific and Technical Information of China (English)

    姜丽; 窦祖林; 温红梅; 兰月; 李奎; 丘卫红; 胡昔权

    2010-01-01

    目的 探讨脑卒中恢复期偏瘫患者肌力训练对大腿肌群表面肌电(sEMG)信号特征的影响,为脑卒中患者的康复治疗提供客观依据.方法 共选取35例脑卒中恢复期偏瘫患者,分为治疗组(n=19)和对照组(n=16),治疗组给予6周的肌力训练,并在治疗前后在患侧膝关节屈伸最大等长收缩(MIVC)时记录股内侧肌、股直肌、股外侧肌、股二头肌、半腱半膜肌的sEMG信号,计算膝关节屈伸力矩值、均方根值(RMS)及其相应协同收缩率;对照组不给予康复训练,仅与治疗组同时进行上述指标测量.结果 治疗组治疗后患者患侧屈膝及伸膝MIVC力矩均较治疗前明显改善[屈膝:(18.02±6.52)nm比(13.12±5.79)nm,伸膝:(45.72±17.21)nm比(34.76±17.19)nm,均P<0.05],而伸屈膝协同收缩率较治疗前无明显变化.对照组伸屈膝MIVC力矩和协同收缩率治疗前后差异则无统计学意义(均P>0.05).治疗组治疗后患者患侧大腿股直肌、股外侧肌、半腱半膜肌作为主动肌时的RMS值均较治疗前明显改善[(146.60±60.85)μV比(97.02±57.17)μV,(172.65±60.73)μV比(131.46±52.15)μV,(188.69±89.60)μV比(130.57±73.76)μV,均P<0.05],而股内侧肌、股二头肌的RMS值在治疗前后无明显变化,对照组治疗前后各肌的RMS值则无变化(均P>0.05).结论 肌力训练可改善脑卒中恢复期偏瘫患者下肢屈伸肌力,但并不会增强下肢伸屈肌的异常收缩.sEMG结合力矩测量能更全面评估偏瘫肢体功能状态.%Objective To explore the effect of muscle strength exercise on surface electromyography (sEMG) over thigh in stroke patients with hemiplegia during convalescence so as to gain objective evidence for rehabilitation therapy of stroke patients. Methods Thirty-five stroke patients with hemiplegia during convalescence were enrolled in this study, and were divided into two groups, treatment group (n=19) and control group (n=16). The treatment group underwent muscle

  16. 低剂量CT灌注成像在康复期缺血性卒中患者中的应用价值%The Application Value of Low Dose CT Perfusion Imaging in Patients with Ischemic Stroke of Convalescence

    Institute of Scientific and Technical Information of China (English)

    付冬

    2016-01-01

    Objective To study the application value of low dose CT perfusion imaging in patients with ischemic stroke of convalescence.Methods One hundred patients with recovery stage of ischemic stroke were selected as object of study from March 2013 to March 2015 in our hospital. According to the stochastic indicator method, the patients were divided into observation group and control group of 50 cases each. in the two groups of patients, on the basis of routine scan control group using conventional dose (120 kv, 200 mvas) CT perfusion imaging, observation group with low doses (80 kv, 200 mvas) CT perfusion imaging. The two groups were measured in patients with cerebral blood flow (CBF), cerebral blood volume (CBV), mean transit time (MMT), time to peak (TTP).Results Compared two groups of patients, significantly lower rCBF of ischemic lesions central area, the difference was statistically significant (P0.05), compared two groups of patients significantly lower rCBV ischemic lesions central area, the difference was statistically significant (P0.05), compared two groups of patients with the same parts of rCBV, there was no statistically significant difference (P>0.05), compared two groups of patients, a significantly lower rCBV ischemic lesions central area, the difference was statistically significant (P0.05)两组患者相比,病灶中心区rCBV明显低于缺血区,差异有统计学意义(P<0.05),不同组别相同部位的rCBV基本一致,差异无统计学意义(P>0.05)两组患者相比,相同部位的rCBV基本一致,差异无统计学意义(P>0.05),两组患者相比,病灶中心区rCBV明显低于缺血区,差异有统计学意义(P<0.05)。结论低剂量CT灌注成像,在有效降低辐射剂量的同时,能够有效评价患者病灶处的血流情况,对临床治疗以及预后恢复具有重要意义。

  17. The related correlation of mental characteristics,coping style,social support and mental health between schizophrenia primary affection and convalescent schizophrenia%首发恢复期精神分裂症患者人格特征、应对方式及社会支持与心理健康的相关研究

    Institute of Scientific and Technical Information of China (English)

    刘竹华; 李秀英; 王媛; 郭爱宁

    2014-01-01

    合康复计划时,必须基于整合医学视角,积极关注其个性特征、心理健康状况、应对方式、社会支持等方面.%Objective By exploring difference of mental characteristics,coping style,social support and health between schizophrenia primary affection and convalescent schizzy,it's expected that the related rationale will be developed for further formulating treatment for schizophrenia primary affection and convalescent schizophrenia.Methods By taking advantage of symptom checklist,questionnaire of NEO-FFI,Simplified Coping Style Questionnaire,social support scale,we compared 150 schizophrenia primary affection who were under the clinic of new antipsychotics and in convalescent period (schizzy group)with 169 normal persons (normal group)on the mental characteristics,coping style,social support and mental health of individual and correlation.Results The following factors of scl-90 showed statistical significance between patient group and normal group:somatization (1.83±0.14) vs.(1.27-±0.48),interpersonal sensitivity(1.96±0.21) vs.(1.65±0.51),depression(1.89±0.24) vs.(1.50±0.59),anxiety(1.69±0.15) vs.(1.39±0.43),photic anxiety (1.56±0.13) vs.(1.23±0.41),psychoticism factors (1.56± 0.14) vs.(1.23±0.41) (all P<0.01).The personality traits differences between patient group and normal group were as follows:neuroticism (3.13± 0.15) vs.(2.61 ± 0.36),extraversion (2.96± 0.31) vs.(3.19± 0.23),agreeableness (3.20± 0.25) vs.(3.48±0.35) and conscientiousness (3.19±0.65) vs.(3.42± 0.16),and all these factors showed statistical significance with P<0.01.The differences of subjective support factors between patients group and normal groups were as follows:subjective support (23.51 ±3.62) vs.(26.29±3.91),objective support (7.35±2.07) vs.(8.91 ± ±2.89),support use (7.61± 1.23) vs.(8.97± 1.35) and total score of supports (37.63± 6.52) vs.(43.51 ± 6.32) and the scores of patients group were poorer than those of normal group(P<0

  18. [Convalescence and decline in physical function level following intensive therapy

    DEFF Research Database (Denmark)

    Poulsen, J.B.; Moller, K.; Perner, A.

    2009-01-01

    More patients survive critical illness, which emphasises the need to assess outcome measures other than mortality. A prolonged decline in physical function is frequently observed after discharge in the critically ill. Neuromuscular dysfunction and muscle atrophy incurred during intensive care may...

  19. Cardiovascular and respiratory changes and convalescence in laparoscopic colonic surgery

    DEFF Research Database (Denmark)

    Schulze, S; Lyng, K M; Bugge, K

    1999-01-01

    Gasless laparoscopy produces smaller cardiopulmonary and systemic changes than carbon dioxide (CO2) laparoscopy during colonic surgery.......Gasless laparoscopy produces smaller cardiopulmonary and systemic changes than carbon dioxide (CO2) laparoscopy during colonic surgery....

  20. The effects of gradual functional respiratory training on the lung function and quality of life of patients convalescing from Guilliain-Barré syndrome%渐进式呼吸功能训练对格林-巴利综合征恢复期患者肺功能及生活质量的影响

    Institute of Scientific and Technical Information of China (English)

    李芳; 任秀贞; 陈文静

    2012-01-01

    目的 探讨渐进式呼吸功能训练对格林-巴利综合征患者肺功能及生活质量的影响.方法 共选取格林-巴利综合征患者32例,将其随机分为2组,对照组给予神经内科常规护理,呼吸训练组在此基础上给予胸或腹式呼吸、深呼吸和呼吸操三阶段训练.在治疗2,4个月后,分别采用肺功能检测仪、6 min步行试验及SF-36简明量表对2组患者的肺功能、运动能力和生活质量进行评定.结果 治疗2,4个月后,呼吸训练组与治疗前比较,肺功能指标[包括肺活量(VC)、用力肺活量(FVC)、1秒钟用力呼气容积(FEV1)、最大通气量(MVV)]均逐渐提高,且有统计学意义(P<0.05);6 min步行试验及生活质量所含的8个领域评分均显著增高,有统计学意义(P<0.01).对照组肺功能指标( VC、FVC、FEV1、MVV)数据均逐渐降低,无统计学意义(P>0.05);6 min步行试验及生活质量(躯体功能、心理健康、角色心理、身体疼痛和综合健康)5个领域评分逐渐增高,有统计学意义(P<0.05).结论 格林-巴利综合征患者恢复期给予渐进式呼吸功能训练,能有效阻止因肌力减退所致的肺功能损害,可有效改善患者的肺功能、运动能力及生活质量.%Objective To quantify the effect of gradual respiratory functional training on the lung function and quality of life (QOL) of patients convalescing from Guillain-Barré syndrome (GBS). Methods Thirty-two GBS patients were randomly divided into two groups a control group which received routine nursing and a respiratory function training group.The training was in three stages:thoracic or/and abdominal respiration,deep breathing and respiratory exercise.After 2 and 4 months of treatment,the 16 min walk test and the SF-36 health questionnaire were used to evaluate the subjects' lung function,motor capacity and QOL. Results After 2 and 4 months of treatment,vital capacity (VC),forced vital capacity (FVC),forced expiratory volume in

  1. Clinical research of hypnotic therapy in treatment of patients with mental disorders due to alcohol in convalescent period%催眠疗法在酒精所致精神障碍患者康复期治疗中的临床研究

    Institute of Scientific and Technical Information of China (English)

    王伟; 张撰荣; 冯合星; 王晨光; 白萍; 殷文英; 王艳

    2014-01-01

    目的:观察催眠疗法对酒精所致精神障碍患者康复期的作用,探讨治疗酒精所致精神障碍的新疗法。方法:通过前瞻性对照研究,将酒精所致精神障碍患者随机分为研究组和对照组,研究组患者在常规戒酒治疗的基础上,利用催眠疗法进行干预,对照组患者单用常规戒酒治疗。治疗1个月前后分别采用明尼苏达个性量表( MMPI)、汉密尔顿焦虑量表( HAMA)和汉密尔顿抑郁量表(HAMD)作为测查工具,进行回顾性分析。结果:研究组酒精所致精神障碍患者除 Sc、Si 量表外,其他 MMPI 量表的 t 分均比治疗前显著降低(P 均<0.05)。研究组治疗前后 Hs、D、Hy、Pd、Mf、Pa、Pt、Ma 量表的 t 分减分值与对照组比较,差异有统计学意义(P 均<0.05)。治疗前后两组患者 HAMA、HAMD减分均显著降低(P 均<0.05),研究组患者减分率与对照组比较差异有统计学意义(P 均<0.05)。结论:综合运用催眠疗法对酒精所致精神障碍患者的治疗效果显著,无不良反应,值得临床推广。%Objective:To explore effects of hypnotic therapy in treatment of patients with mental disorders due to alcohol in convalescent period and explore new therapies for the treatment of mental disorders due to alcohol. Methods:Through a prospective control study, the patients with mental disorders due to alcohol were selected and randomly divided into research group and control group, wherein the cases in research group were given the hypnosis intervention on the basis of the conventional alcohol withdrawal treatment, whereas those in control group were only given the conventional alcohol withdrawal treatment. Before and 1 month after the treatment, Minnesota personality inventory (MMPI), Hamilton anxiety scale (HAMA) and Hamilton depression scale (HAMD) were used for the retrospective analysis. Results: For research group, except Sc and Si scales, the other T points in MMPI scale

  2. Short convalescence and minimal pain after out-patient Bascom's pit-pick operation

    DEFF Research Database (Denmark)

    Colov, Emilie Palmgren; Bertelsen, Claus Anders

    2011-01-01

    Treatment of pilonidal sinuses with Bascom's pit-pick operation can easily be performed under local analgesia. We describe pain during and after the operation, time to return to work, time to healing and success rate.......Treatment of pilonidal sinuses with Bascom's pit-pick operation can easily be performed under local analgesia. We describe pain during and after the operation, time to return to work, time to healing and success rate....

  3. The effect of balanced analgesia on early convalescence after major orthopaedic surgery

    DEFF Research Database (Denmark)

    Møiniche, S; Hjortsø, N C; Hansen, B L

    1994-01-01

    Forty-two patients scheduled for total knee arthroplasty (n = 20) or hip arthroplasty (n = 22) were randomly allocated to receive either continuous epidural bupivacaine/morphine for 48 h postoperatively plus oral piroxicam, or general anaesthesia followed by a conventional intramuscular opioid an...

  4. Designing for "the little convalescents": children's hospitals in Toronto and Montreal, 1875-2006.

    Science.gov (United States)

    Adams, Annmarie; Theodore, David

    2002-01-01

    This paper explores more than a century of changing ideas about the health of Canadian children through the architecture of pediatric hospitals in Montreal and Toronto. As a unique source in the history of medicine, hospital architecture reveals three distinct phases in the construction of children as patients. Early 20th-century children's hospitals remained bastions of older spatial attitudes towards health. The postwar hospital was self-consciously modern, with an arrangement more scientific and institutional than its predecessor. Through reference to other typologies, the postmodern hospital marks a curious return to the earlier attitude that children's health is a family affair. Is the hospital a home for children or an institution for science?

  5. The effect of balanced analgesia on early convalescence after major orthopaedic surgery

    DEFF Research Database (Denmark)

    Møiniche, S; Hjortsø, N C; Hansen, B L;

    1994-01-01

    Forty-two patients scheduled for total knee arthroplasty (n = 20) or hip arthroplasty (n = 22) were randomly allocated to receive either continuous epidural bupivacaine/morphine for 48 h postoperatively plus oral piroxicam, or general anaesthesia followed by a conventional intramuscular opioid...

  6. Reduced length of stay and convalescence in laparoscopic vs open sigmoid resection with traditional care

    DEFF Research Database (Denmark)

    Kaltoft, B; Gögenur, I; Rosenberg, J

    2011-01-01

    The effect of a laparoscopic technique without a multi-modal rehabilitation programme but with traditional postoperative care was studied in a blinded randomized trial regarding nursing time, hospital stay, pain, fatigue, need for sleep and return to normal daily activities.......The effect of a laparoscopic technique without a multi-modal rehabilitation programme but with traditional postoperative care was studied in a blinded randomized trial regarding nursing time, hospital stay, pain, fatigue, need for sleep and return to normal daily activities....

  7. Reduced length of stay and convalescence in laparoscopic vs open sigmoid resection with traditional care

    DEFF Research Database (Denmark)

    Kaltoft, B; Gögenur, I; Rosenberg, J

    2011-01-01

    The effect of a laparoscopic technique without a multi-modal rehabilitation programme but with traditional postoperative care was studied in a blinded randomized trial regarding nursing time, hospital stay, pain, fatigue, need for sleep and return to normal daily activities.......The effect of a laparoscopic technique without a multi-modal rehabilitation programme but with traditional postoperative care was studied in a blinded randomized trial regarding nursing time, hospital stay, pain, fatigue, need for sleep and return to normal daily activities....

  8. Impact of Polypharmacy on the Rehabilitation Outcome of Japanese Stroke Patients in the Convalescent Rehabilitation Ward

    Directory of Open Access Journals (Sweden)

    Eiji Kose

    2016-01-01

    Full Text Available Background. A risk factor associated with stroke onset is chronic kidney disease (CKD. To prevent stroke reoccurrence, it is necessary to strictly manage blood pressure, lipids, and plasma glucose. Therefore, some cases are forced to polypharmacy, elderly patients in particular. Polypharmacy often leads to adverse drug reactions and has the potential to negatively affect the rehabilitation of stroke patients. The aim of the present study was to investigate the effects of polypharmacy using a functional independence measure (FIM. Methods. A total of 144 stroke patients with CKD were included in the present analysis. We divided stroke patients into those taking six or more drugs (polypharmacy group and those taking less than six drugs (nonpolypharmacy group upon admission. Patient background features, laboratory data, and FIM scores were compared. Results. FIM-Motor (FIM-M efficiency, age, and diabetes mellitus were positively associated with polypharmacy. FIM-M efficiency in the polypharmacy group was significantly lower than in the nonpolypharmacy group. Conclusion. Polypharmacy interferes with the effect of rehabilitation in stroke patients with CKD. Pharmacists and doctors should make efforts to optimize medications to be able to respond to the outcome of each patient.

  9. THE ORGANIZATION OF REABILITATION DEPARTMENTS FOR CHILDREN — CONVALESCENT INFECTIOUS DISEASES

    Directory of Open Access Journals (Sweden)

    E. V. Pronina

    2014-01-01

    Full Text Available Recently the actual direction is development of programs of rehabilitation of the second stage of recovering of infectious diseases children. The purpose of this work is justification of need of the organization of offices of rehabilitation in a children's infectious hospital according to Order of the organization of medical rehabilitation. The retrospective analysis of clinical records of the patients who have arrived on treatment in clinical divisions of Federal State Budgetary Institution of Research of Children's Infections of Russia in 2011—2012 is carried out. It is established, that among surveyed 14 363 for 2 years of patients (in a year on the average more than 7000 children — about 40% (2800 patients a year children for whom rehabilitation actions of the second stage in the conditions of a hospital, including carrying out a diet therapy, physiotherapy exercises, physical therapy and pharmacotherapy are necessary. On a profile of infectious patients for rendering the rehabilitation help it is expedient to organize 2 offices on 30 beds: for treatment of children with consequences of neuroinfections and children with somatic pathology. The organization of two offices for carrying out medical rehabilitation of the second stage to recovering infectious diseases children taking into account recommended standards on equipment, according to the Order of the organization of medical rehabilitation, will allow to give high-tech help to patients and to prevent development of complications.

  10. Impressions of health in the new South Africa: a period of convalescence.

    Science.gov (United States)

    Kale, R

    1995-04-29

    The White minority population in South Africa long oppressed and disenfranchised the country's Black majority. The apartheid system, however, was finally put to rest with the election of Nelson Mandela as President of South Africa during April 27-29, 1994. During apartheid, the White minority enjoyed the best health care available among developed countries, while Blacks were forced to receive some of the worst care provided in the Third World. The doctor/patient ratios in 1981 for White and Black patients, respectively, were 330 and 91,000. In 1985, infant mortality for White infants was 13.1/1000 compared to 70/1000 for Blacks. The existence of fourteen separate health departments caused major duplication and waste. South Africa now has the opportunity to effect revolutionary changes in its health care. To that end, President Mandela announced free health care for pregnant women and children younger than age six years on May 24, 1994. A subsequent uptake in health service utilization was quickly observed in small towns. Structurally, a central health department with a provincial department for each of the four provinces replaced the redundant predemocracy system of fourteen health departments. This change, however, for now, exists only on paper and net financial gains from the reorganization have yet to be realized. Finally, there have been frequent strikes in hospitals throughout the country by health workers demanding better pay and working conditions.

  11. Loss of Humoral and Cellular Immunity to Invasive Nontyphoidal Salmonella during Current or Convalescent Plasmodium falciparum Infection in Malawian Children.

    Science.gov (United States)

    Nyirenda, Tonney S; Nyirenda, James T; Tembo, Dumizulu L; Storm, Janet; Dube, Queen; Msefula, Chisomo L; Jambo, Kondwani C; Mwandumba, Henry C; Heyderman, Robert S; Gordon, Melita A; Mandala, Wilson L

    2017-07-01

    Invasive nontyphoidal Salmonella (iNTS) infections are commonly associated with Plasmodium falciparum infections, but the immunologic basis for this linkage is poorly understood. We hypothesized that P. falciparum infection compromises the humoral and cellular immunity of the host to NTS, which increases the susceptibility of the host to iNTS infection. We prospectively recruited children aged between 6 and 60 months at a Community Health Centre in Blantyre, Malawi, and allocated them to the following groups; febrile with uncomplicated malaria, febrile malaria negative, and nonfebrile malaria negative. Levels of Salmonella enterica serovar Typhimurium-specific serum bactericidal activity (SBA) and whole-blood bactericidal activity (WBBA), complement C3 deposition, and neutrophil respiratory burst activity (NRBA) were measured. Levels of SBA with respect to S Typhimurium were reduced in febrile P. falciparum-infected children (median, -0.20 log10 [interquartile range {IQR}, -1.85, 0.32]) compared to nonfebrile malaria-negative children (median, -1.42 log10 [IQR, -2.0, -0.47], P = 0.052). In relation to SBA, C3 deposition on S Typhimurium was significantly reduced in febrile P. falciparum-infected children (median, 7.5% [IQR, 4.1, 15.0]) compared to nonfebrile malaria-negative children (median, 29% [IQR, 11.8, 48.0], P = 0.048). WBBA with respect to S Typhimurium was significantly reduced in febrile P. falciparum-infected children (median, 0.25 log10 [IQR, -0.73, 1.13], P = 0.0001) compared to nonfebrile malaria-negative children (median, -1.0 log10 [IQR, -1.68, -0.16]). In relation to WBBA, S Typhimurium-specific NRBA was reduced in febrile P. falciparum-infected children (median, 8.8% [IQR, 3.7, 20], P = 0.0001) compared to nonfebrile malaria-negative children (median, 40.5% [IQR, 33, 65.8]). P. falciparum infection impairs humoral and cellular immunity to S Typhimurium in children during malaria episodes, which may explain the increased risk of iNTS observed in children from settings of malaria endemicity. The mechanisms underlying humoral immunity impairment are incompletely understood and should be explored further. Copyright © 2017 Nyirenda et al.

  12. Framing medical tourism: an examination of appeal, risk, convalescence, accreditation, and interactivity in medical tourism web sites.

    Science.gov (United States)

    Mason, Alicia; Wright, Kevin B

    2011-02-01

    This exploratory study analyzed the content of medical tourism Web sites in an attempt to examine how they convey information about benefits and risks of medical procedures, how they frame credibility, and the degree to which these Web sites include interactive features for consumers. Drawing upon framing theory, the researchers content analyzed a sample of 66 medical tourism Web sites throughout the world. The results indicated that medical tourism Web sites largely promote the benefits of medical procedures while downplaying the risks, and relatively little information regarding the credibility of these services appears. In addition, the presentation of benefits/risks, credibility, and Web site interactivity were found to differ by region and type of facility. The authors discuss the implications of these findings concerning the framing of medical tourism Web site content, future directions for research, and limitations.

  13. Struggling for Independence: A Grounded Theory Study on Convalescence of ICU-survivors 12 Months Post ICU Discharge

    OpenAIRE

    Ågård, Anne Sophie; Egerod, Ingrid; Tønnesen, Else Kirstine; Lomborg, Kirsten

    2012-01-01

    Objectives: To explore and explain the challenges, concerns and coping modalities in ICU-survivors living with a partner or spouse during the first 12 months post ICU discharge. Design: Qualitative, longitudinal grounded theory study.Settings: Five ICUs in Denmark, four general, one neurosurgical. Methods: Thirty-five interviews with patients and their partners at three and 12 months post ICU-discharge plus two group interviews with patients only and two with partners only.Findings: The ICU-s...

  14. Intraarticular glucocorticoid, morphine and bupivacaine reduces pain and convalescence after arthroscopic ankle surgery: a randomized study of 36 patients

    DEFF Research Database (Denmark)

    Rasmussen, S; Kehlet, H

    2000-01-01

    In a double-blind randomized study, 36 patients undergoing arthroscopic removal of bony spurs and synovitis causing impingement of the ankle were allocated to intraarticular saline or bupivacaine 15 mg + morphine 5 mg + intraarticular methylprednisolone 40 mg. Combined methylprednisolone...

  15. Clinical Effectiveness of Board-certificated Physiatrists on Functional Recovery in Elderly Stroke Patients During Convalescence: A Retrospective Cohort Study

    Directory of Open Access Journals (Sweden)

    Ryo Momosaki

    2017-03-01

    Conclusions: Our results suggest that clinical management provided to elderly stroke patients by board-certificated physiatrists is associated with good functional improvement. Board certification should be encouraged to improve the quality of rehabilitative management of post-stroke patients.

  16. Disfagia orofaríngea en ancianos ingresados en una unidad de convalecencia Oropharyngeal dysphagia in elderly inpatients in a unit of convalescence

    Directory of Open Access Journals (Sweden)

    L. J. Silveira Guijarro

    2011-06-01

    Full Text Available Objetivo principal: Describir la prevalencia de disfagia orofaríngea al alta en ancianos ingresados en una Unidad de Subagudos (USA usando el Método de Evaluación Clínica Volumen-Viscosidad (MECV-V y una versión adaptada en demencia grave (MECV-V-G. Metodología y diseño: Estudio transversal, descriptivo; periodo: 50 días. Datos de la historia clínica de todos los pacientes al alta hospitalaria: demográficos, clínicos, factores de riesgo y complicaciones de disfagia, evolución funcional y resultados del MECV-V y MECV-V-G. Se describen comparando características de los grupos con y sin disfagia. Resultados: 86 pacientes (60% mujeres, edad media 83,8 ± 6,7 años. La anamnesis dirigida detectó disfagia orofaríngea previa en 23 pacientes (26%. El MECV-V detectó disfagia orofaríngea en 46 pacientes (53,5%. De ellos 30 pacientes (65,21% tuvieron trastorno mixto de deglución, 15 (32,6% trastorno aislado de eficacia y 1 (2,17% trastorno aislado de seguridad. Aquellos con test de disfagia positivo, tenían, de manera estadísticamente significativa, mayor prevalencia de deterioro cognitivo, mayor edad, más antecedentes de disfagia previa, peor evolución funcional y de movilidad y más complicaciones durante su estancia en USA. Conclusiones: La disfagia es altamente prevalente en este grupo de ancianos hospitalizados. Mediante la anamnesis dirigida sólo se diagnóstica la mitad de los casos. El MECV-V detectó una alta prevalencia de disfagia que recomienda su uso rutinario especialmente en pacientes de riesgo teniendo en cuenta las peculiaridades de utilización en ancianos. Dicha población de riesgo vendría definida por características como mayor edad, deterioro cognitivo y/o funcional.Main objective: To describe the prevalence of oropharyngeal dysphagia at hospital discharge in elderly patients admitted to a Subacute Care Unit (SACU using the Volume-Viscosity Swalow Test (V-VST and an adapted version for severe dementia (V-VST-G. Methodology and design: Descriptive cross-sectional study; duration; 50 days. Data gathered from the clinical chart at hospital discharge: demographical, clinical, risk factors, and complications of dysphagia, functional course, and V-VCAM and V-VCAM-G outcomes. The results are described comparing the data of the groups with and without dysphagia. Results: 86 patients (60% women, mean age 83.8 ± 6.7 years. The specific clinical history detected previous oropharyngeal dysphagia in 23 patients (26%. The V-VCAM detected oropharyngeal dysphagia in 46 patients (53.5%. Of them, 30 patients (65.21% had mixed swallowing disorder, 15 (32.6% had isolated efficacy disorder, and 1 (2.17% had isolated safety disorder. Those patients with a positive dysphagia test had a statistically significant higher prevalence of cognitive disorder, higher age, and more positive history of previous dysphagia, worse functional course and mobility impairment, and more complications during their staying at the SACU. Conclusions: dysphagia is highly prevalent among this group of elderly patients. Only half of the cases are diagnosed through the specific anamnesis. The V-VCAM detected a high prevalence of dysphagia so that its routine use is recommended specially in patients at risk taking into account the peculiarities of using it in the elderly. This at-risk population would be defined by characteristics such as higher age, cognitive and/or functional impairment.

  17. Detection of Foot-and-Mouth Disease Virus RNA and capsid protein in lymphoid tissues of convalescent pigs does not indicate existence of a carrier state

    Science.gov (United States)

    A systematic study was performed to investigate the potential of pigs to maintain persistent Foot-and-Mouth Disease Virus (FMDV) infection. Infectious virus could not be recovered from sera, oral, nasal- or oropharyngeal fluids obtained after resolution of clinical infection with FMDV serotypes A, O...

  18. Impact of the number of painful stimuli on life satisfaction among Korean industrial accident workers completing convalescence: dual mediating effects of self-esteem and sleeping time

    National Research Council Canada - National Science Library

    CHOI, Wan-Suk; KIM, Bo-Kyung; KIM, Ki-Do; MOON, Ok-Kon; YEUM, Dong-Moon

    2016-01-01

    This study examined the impact of the number of painful stimuli on life satisfaction among workers who experienced an industrial accident and investigated how self-esteem and sleeping time affected life satisfaction...

  19. Residual Myocardial Iron Following Intramyocardial Hemorrhage During the Convalescent Phase of Reperfused ST-Segment–Elevation Myocardial Infarction and Adverse Left Ventricular Remodeling

    Science.gov (United States)

    Bulluck, Heerajnarain; Rosmini, Stefania; Abdel-Gadir, Amna; White, Steven K.; Bhuva, Anish N.; Treibel, Thomas A.; Fontana, Marianna; Ramlall, Manish; Hamarneh, Ashraf; Sirker, Alex; Herrey, Anna S.; Manisty, Charlotte; Yellon, Derek M.; Kellman, Peter; Moon, James C.

    2016-01-01

    Background— The presence of intramyocardial hemorrhage (IMH) in ST-segment–elevation myocardial infarction patients reperfused by primary percutaneous coronary intervention has been associated with residual myocardial iron at follow-up, and its impact on adverse left ventricular (LV) remodeling is incompletely understood and is investigated here. Methods and Results— Forty-eight ST-segment–elevation myocardial infarction patients underwent cardiovascular magnetic resonance at 4±2 days post primary percutaneous coronary intervention, of whom 40 had a follow-up scan at 5±2 months. Native T1, T2, and T2* maps were acquired. Eight out of 40 (20%) patients developed adverse LV remodeling. A subset of 28 patients had matching T2* maps, of which 15/28 patients (54%) had IMH. Eighteen of 28 (64%) patients had microvascular obstruction on the acute scan, of whom 15/18 (83%) patients had microvascular obstruction with IMH. On the follow-up scan, 13/15 patients (87%) had evidence of residual iron within the infarct zone. Patients with residual iron had higher T2 in the infarct zone surrounding the residual iron when compared with those without. In patients with adverse LV remodeling, T2 in the infarct zone surrounding the residual iron was also higher than in those without (60 [54–64] ms versus 53 [51–56] ms; P=0.025). Acute myocardial infarct size, extent of microvascular obstruction, and IMH correlated with the change in LV end-diastolic volume (Pearson’s rho of 0.64, 0.59, and 0.66, respectively; P=0.18 and 0.62, respectively, for correlation coefficient comparison) and performed equally well on receiver operating characteristic curve for predicting adverse LV remodeling (area under the curve: 0.99, 0.94, and 0.95, respectively; P=0.19 for receiver operating characteristic curve comparison). Conclusions— The majority of ST-segment–elevation myocardial infarction patients with IMH had residual myocardial iron at follow-up. This was associated with persistently elevated T2 values in the surrounding infarct tissue and adverse LV remodeling. IMH and residual myocardial iron may be potential therapeutic targets for preventing adverse LV remodeling in reperfused ST-segment–elevation myocardial infarction patients.

  20. 细化老年性耳聋疗养员护理服务流程%Elaboration of the nursing service process for presbycusis convalescents

    Institute of Scientific and Technical Information of China (English)

    栾春花

    2011-01-01

    本文介绍了对年龄在70岁以上患有老年性耳聋的50例疗养员,根据他们听力差的个性特点,针对性地细化每个阶段的护理服务内容,实施从入院、疗养、出院3个阶段全程护理服务,使患有老年性耳聋的疗养员通过疗养不但促进了身体康复,还提高了对生活的自信心.通过发放疗养员征求意见表,疗养员对我科护理服务的满意率达到99%以上,此流程赢得了疗养员的赞誉,同时也提升了我科护理服务的整体水平,使我科护理服务工作进一步走上了规范化管理的轨道.

  1. Elementary Rehabilitation Nursing Care; a Manual for Nurses and Ancillary Workers in Nursing Homes, Hospitals, Convalescent Facilities, and Public Health Agencies. Public Health Service Publication No. 1436.

    Science.gov (United States)

    Colorado State Dept. of Public Health, Denver. Public Health Nursing Section.

    This guide for teacher and student use presents a comprehensive program of physical rehabilitation for aged and physically disabled patients. Developed by the Public Health Nursing Section, the manual was tested by state health department personnel and persons doing inservice teaching in their respective nursing homes. The program is designed to…

  2. Thin-Section Computed Tomography Manifestations During Convalescence and Long-Term Follow-Up of Patients with Severe Acute Respiratory Syndrome (SARS).

    Science.gov (United States)

    Wu, Xiaohua; Dong, Dawei; Ma, Daqing

    2016-08-08

    BACKGROUND SARS is not only an acute disease, but also leads to long-term impaired lung diffusing capacity in some survivors. However, there is a paucity of data regarding long-term CT findings in survivors after SARS. The aim of this study was to assess the changes in lung function and lung thin-section computed tomography (CT) features in patients recovering from severe acute respiratory syndrome (SARS), especially the dynamic changes in ground-glass opacity (GGO). MATERIAL AND METHODS Clinical and radiological data from 11 patients with SARS were collected. The serial follow-up thin-section CTs were evaluated at 3, 6, and 84 months after SARS presentation. The distribution and predominant thin-section CT findings of lesions were evaluated. RESULTS The extent of the lesions on the CT scans of the 11 patients decreased at 6 and 84 months compared to 3 months. The number of segments involved on 84-month follow-up CTs was less than those at 6 months (PSARS, GGO and intralobular and interlobular septal thickening were the main thin-section CT manifestation. Intralobular and interlobular septal thickening predominated over GGO at 84 months.

  3. Clinical Guidance on Functional Exercise of Patients with Burns in Convalescence%烧伤康复期患者功能锻炼的临床指导

    Institute of Scientific and Technical Information of China (English)

    洪梅

    2009-01-01

    目的:探讨大面积烧伤患者康复期的功能锻炼方法.方法:回顾分析77例大面积烧伤患者康复期患者的功能锻炼.结果:77例患者均最大限度恢复功能,取得满意效果.结论:针对大面积烧伤患者康复期存在的问题,有目的地进行临床指导,可最大程度恢复功能.

  4. 延续性护理对冠心病康复期患者生活质量的影响%Influence of Nursing Intervention on Quality of Life of Coronary Heart Disease Continues PCI Convalescent Patients

    Institute of Scientific and Technical Information of China (English)

    门秀珍

    2015-01-01

    Objective To investigate the effect of nursing continuing on quality of life of coronary heart disease patients . Methods percutaneous coronary intervention (PCI) in 80 patients with coronary heart disease ,were randomly divided into the observation group 40 cases and control group .The patients in the control group were given routine nursing care ,while using the continuity of care in the study group with social disability screening table (Social Disability Screeningschedule , SDSS) ,questionnaire of quality of life in patients with cardiovascular disease (CCQQ ) .And Chinese medicine adherence scale score between the two groups were compared before and after nursing medication compliance ,the social function and quality of life .Results Nursing intervention of 6 months later ,Activity ,dependence ,social ability ,the symptoms and SDSS scores in the observation group were significantly lower than those in the control group (P 0 .05) in the evaluation results of CCQQ .6 months after care ,general life function scores (9 .21 ± 0 .36) in the observation group ,psychological and social function scores (20 .14 ± 3 .08) ,work status (5 .32 ± 0 .51) ,medical conditions score (5 .12 ± 0 .45) ,physical score (15 .80 ± 2 .17) ,condition score (16 .23 ± 2 .46) .The CCQQ scores were increased in patients of two groups before nursing (P < 0 .05) ,but it increased obviously (P < 0 .05) in the ob‐servation group .Conclusion the continuing nursing care can effectively improve the exercise tolerance in patients with cor‐onary heart disease ,social function and life quality ,promote the rehabilitation of patients .It is worthy of clinical applica‐tion .%目的:探讨延续性护理对冠心病康复期患者生活质量的影响。方法选取80例冠心病患者,随机分为对照组和观察组,各40例。对照组采用常规护理。观察组采用延续性护理。采用社会功能缺陷筛选量表(SDSS )、中国心血管病人生活质量评定问卷(CCQQ)、服药依从性量表进行评分。比较两组护理前后服药依从性、社会功能及生活质量的差异。结果护理干预6个月后观察组完全按时服药31例,占77.5%,部分按时7例,占17.5%,服药依从率为95%,观察组服药依从性显著高于对照组(Z=-4.430,P<0.01);两组护理前CCQQ评分比较无显著性差异(P>0.05);护理干预6个月后,观察组一般生活功能评分为(9.21±0.36)、社会心理功能评分为(20.14±3.08)、工作状况评分为(5.32±0.51)、医疗情况评分为(5.12±0.45)、体力评分为(15.80±2.17)、病情评分为(16.23±2.46);两组CCQQ评分较护理前均有所提高(P<0.05),但观察组升高幅度明显较大(P<0.05);护理干预6个月后,观察组活动能力、依赖程度、社交能力、目前症状以及SDSS总分显著低于对照组(P<0.05)。结论延续性护理可以有效提高冠心病患者的服药依从性、改善社会功能及生活质量,促进患者康复。

  5. 恢复期SARS患者外周血T淋巴细胞及亚群的变化%Changes of T lymphocytes and subgroups in peripheral blood in convalescent SARS patients

    Institute of Scientific and Technical Information of China (English)

    伍伟玲; 程涛; 李灼亮

    2004-01-01

    目的:追踪观察严重急性呼吸综合征(SARS)患者恢复后外周血CD3+、CD4+、CD8+T淋巴细胞及机体的免疫状况变化.方法:10例健康人和9例恢复期SARS患者采静脉血,用流式细胞仪检测总T淋巴细胞(CD3+)、CD4+、CD8+T淋巴细胞.结果:全部患者外周血CD8+T淋巴细胞均恢复正常;恢复后SARS患者外周血总CD3+T淋巴细胞和CD4+、CD8+T淋巴细胞与对照组比较差异无显著性.结论:恢复期SARS患者外周血总T淋巴细胞和CD4+、CD8+T淋巴细胞有不同程度的恢复,SARS病毒感染后机体免疫功能的损害呈暂时性.

  6. Effects of residential style on quality of life among retired military convalescents%居住模式对军队离退休疗养员生存质量的影响

    Institute of Scientific and Technical Information of China (English)

    李云贵; 郑军; 吴运辉; 鄢娟; 张伟杰

    2006-01-01

    目的了解居住模式对军队离退休疗养员生存质量的影响.方法采用及自编的对324名军队离退休疗养员进行调查.结果 (1)军队离退休疗养员生存质量各领域得分均高于地方社区人群.(2)采用"分开住,子女常回家"这种居住模式的离退休疗养员生存质量各领域得分均最高.结论不同的居住模式对离退休疗养员的生存质量有影响.

  7. 团体心理辅导在强戒人员康复期作用的反思%Reflections on the Role of Group Psychological Counseling in the Convalescence of Drug Addicts under Compulsory ;Rehabilitation

    Institute of Scientific and Technical Information of China (English)

    周薇

    2015-01-01

    团体心理辅导是一种非常重要的心理健康教育形式。在强制隔离戒毒场所开展戒毒人员团体心理辅导可以有效地提高戒毒人员的心理健康水平,使得心理健康教育工作开展得更加全面、高效和深入。团体心理辅导在戒毒人员心理康复工作中也越来越受到重视。文章从团体辅导实施、经验总结和建议三方面反思了我国戒毒人员团体心理辅导过程中的情况,为以后强制戒毒心瘾戒断工作的开展提供参考。%Group psychological counseling, as a very important form of psychological health education, can effectively improve the psychological health of drug addicts under compulsory reha-bilitation, which contributes to the more comprehensive, effective and further implementation of psychological health education. So group psychological counseling has received more and more at-tention in the psychological rehabilitation of drug addicts under compulsory rehabilitation. This article provides reflections on the implementation and experience of group psychological counseling in China, which supplies references to help drug addicts under compulsory rehabilitation to clear up their psychological depen-dence on drug.

  8. 脑活素高压氧对新生儿缺氧缺血性脑病恢复期的治疗%Treatment of Newborn Hypoxic Ischemic Encephalopathy in Convalescence by Cerebrolysin and Hyperbaric Oxygen

    Institute of Scientific and Technical Information of China (English)

    赵青云

    2005-01-01

    目的探讨恢复期治疗对新生儿缺氧缺血性脑病(HIE)预后的影响.方法收集本院86例中、重度HIE患儿,根据1996年杭州会议修改的HIE临床诊断依据及分度进行诊断和分度.采用急性期HIE综合治疗,病情稳定后开始恢复期治疗,用脑活素配合高压氧治疗.测定神经系统后遗症发生情况,智能发育商数.结果治疗组神经系统后遗症9例,对照组12例,两组对比P<0.01;智能发育商数与未治疗组有显著性差异(P<0.01).结论恢复期高压氧、脑活素治疗新生儿HIE能减少神经系统后遗症发生率,智能发育商有统计学意义,明显改善了预后.

  9. Effects of Treadmill Training on Falling of Convalescent Stroke Patients%踏车运动对恢复期脑卒中偏瘫患者跌倒的临床研究

    Institute of Scientific and Technical Information of China (English)

    闫桂芳; 韩广彦; 槐雅萍; 贾子善; 赵雪平; 宋兰欣; 黄明威

    2008-01-01

    目的 探讨踏车运动对恢复期脑卒中偏瘫患者跌倒的影响.方法 将具有一定行走能力的112例恢复期脑卒中患者随机分为观察组(58例)和对照组(54例),对照组患者采用常规康复训练方法 ,观察组患者在常规康复训练的基础上,增加踏车运动,8周后统计两组患者跌倒的发生率,采用Berg平衡量表(Berg balance scale,BBS)、Motricity指数及Fugl-Meyer运动功能评定(Fugl-Meyer assessment,FMA)对疗效进行评价.结果 治疗前观察组和对照组患者平衡功能、下肢肌力、下肢运动功能及跌倒率无统计学差异(P>0.05),经过8周康复训练后,两组患者的平衡功能、下肢肌力、下肢运动功能均明显提高,与治疗前比较差异均有统计学意义(P<0.05),但观察组提高的程度优于对照组,两组间差异有统计学意义(P<0.05),观察组跌倒率明显低于对照组,差异亦有统计学意义(P<0.05).结论 踏车运动能明显提高脑卒中偏瘫患者平衡功能,对降低恢复期脑卒中患者跌倒率有积极作用.

  10. New Preparations of Ginkgo Biloba in the Thetreatment of Ischemic Stroke at Convalescent Stage for 30 Cases%银杏新制剂治疗缺血性脑卒中恢复期30例

    Institute of Scientific and Technical Information of China (English)

    陈培峰

    2016-01-01

    Objective To observe the therapeutic effect and safety of diterpene lactones of Ginkgo biloba meglumine injection in the treatment of ischemic stroke ( phlegm and blood stasis syndrome ) .Methods 60 cases of patients with stroke at recovery period in our department were randomly divided into treatment group and control group , with 30 cases in each group .The treatment group used diterpene lactones of Ginkgo biloba meglumine injection for intravenous injection .The control group was treated with Salvia miltiorrhiza Ligusticum chuanxiong injection for intravenous drip .The course of treatment was 14 days.The clinical effect of the two groups was compared .Results There were significant differences in clinical effect between the two groups (U=2.238, P=0.012).Conclusion The diterpene lactones of Ginkgo biloba meglumine injection in the treatment of ischemic stroke is safe and effective , and it is worthy to be popularized in clinic .%目的:观察银杏二萜内酯葡胺注射液治疗缺血性脑卒中(痰瘀阻络证)的疗效及安全性。方法将我科室60例脑卒中恢复期患者随机分为治疗组和对照组各30例,治疗组采用银杏二萜内酯葡胺注射液静脉滴注,对照组采用丹参芎嗪注射液静脉滴注,疗程均为14 d。比较两组临床疗效。结果两组临床疗效间差异有统计学意义(u=2.238,P=0.012)。结论银杏二萜内酯葡胺注射液治疗缺血性脑卒中安全有效,值得在临床上推广使用。

  11. Curative effect observation of cognitive behavioral therapy on the convalescent female paranoid schizophrenia anxiety%认知行为疗法对首发女性偏执型精神分裂症康复期焦虑情绪的疗效观察

    Institute of Scientific and Technical Information of China (English)

    和西波; 李志文; 陈玉辉

    2012-01-01

    目的:观察认知行为疗法对首发偏执型精神分裂症康复期女性患者焦虑情绪的疗效.方法:选取首发偏执型精神分裂症康复期患者100例,随机分为研究组与对照组各50例.研究组在抗精神病药物治疗的基础上给予连续28天、每天1个小时的认知行为疗法;对照组只给予原有药物治疗.在研究结束后进行心理治疗,以符合伦理学原则.在治疗前、治疗后以及治疗后12周时进行HAMA量表评分,观察焦虑疗效.结果:研究组脱落15例,对照组脱落10例.研究组35例患者治疗前后HAMA评分分别为(11.03±1.30)分、(4.30±1.84)分,治疗后评分显著低于治疗前,差异具有统计学意义(t=5.083,P=0.005).治疗后研究组HAMA评分(4.30±1.84)分,对照组(5.02±2.33)分,研究组低于对照组,差异具有统计学意义(t=-4.395,P=0.000).12周随访研究组HAMA评分(3.58±1.47)分,仍显著低于治疗前,差异均具有统计学意义(t=4.120,P=0.043).结论:认知行为治疗有助于改善康复期偏执型精神分症女性患者的焦虑情绪,在12周随访时疗效依然存在.

  12. 分散内观认知疗法对女性偏执型精神分裂症康复期患者临床症状的应用研究%Applied Research on Clinical Symptoms of Discontiguous Naikan Cognitive Therapy for Women Paranoid Schizophrenia Convalescence Patients

    Institute of Scientific and Technical Information of China (English)

    梁瑞华; 孙倩倩; 刘小翠

    2014-01-01

    目的:探讨分散内观认知疗法(Discontiguous Naikan Cognitive Therapy,DNCT)合并药物治疗对女性偏执型精神分裂症康复期患者临床症状的改善。方法研究对象为70例住院的女性偏执型分裂症康复期患者,将入组患者随机分为两组,35例研究组患者给予分散内观认知疗法合并药物治疗,对照组35例给予药物治疗,所有患者在治疗前、后采用简明精神病评定量表(BPRS)评定疗效。结果研究组完成32例,对照组完成34例,研究组治疗后BPRS量表总分、思维障碍、迟滞、焦虑抑郁、敌意猜测等因子的评分较治疗前降低,差异有统计学意义;对照组治疗后的迟滞因子分较治疗前降低,差异有统计学意义;治疗后研究组BPRS量表总分、活动过多、敌意猜测、焦虑抑郁等因子的评分较治疗前降低,差异有统计学意义。结论分散内观认知疗法联合药物治疗可以改善偏执型分裂症康复期患者的临床症状。%Objective To investigate the treatment of clinical symptoms with Discontiguous Naikan Cognitive Therapy (DNCT)combined with antipsychotic on the female patients with paranoid schizophrenia in rehabilitation period. Methods 70 female hospitalized patients with paranoid schizophrenia in rehabilitation period were chosen. Al patients were randomly divided into two groups. The study group (35 cases) was given DNCT combined with antipsychotic, and the control group (35 cases) was given antipsychotic . The Brief Psychiatric Rating Scale was administered to al subjects pre-treatment and post-treatment. Results There were 32 and 34 patients respectively in study group and control group finishing the study. In the study group, BPRS total scores after treatment, anxiety and depression factor, damping factor, thought disorder factor and hostile speculation factor scores were lower than before treatment, the dif erence was statistical y significant;In control group, before and after treatment,BPRS retardation factor scale score points lower; In the study group after treatment, BPRS total score, anxiety and depression factors, activities, and hostile speculation factor score were lower than before treatment. Conclusion DNCT combined with antipsychotic can improve the clinical symptoms on the recovery status of paranoid schizophrenia.

  13. Influence of Signed Language Music Therapy on Negative Emotion of Convalescent Schizophrenic Serving Sentence%手语操音乐疗法对服刑康复期精神分裂症患者负性情绪的影响

    Institute of Scientific and Technical Information of China (English)

    罗旭英; 吕春梅; 张建花

    2011-01-01

    目的 探讨手语操音乐疗法对服刑康复期精神分裂症患者负性情绪的影响.方法 将60例男性服刑康复期精神分裂症患者随机分为观察组和对照组各30例.对照组给予一般支持性的心理护理,观察组在此基础上进行为期6周的手语操音乐治疗干预.采用焦虑自评量表(Self-Rating Anxiety Scale,SAS)、抑郁自评量表(Self-Rating Depression Scale,SDS)、护士用住院病人观察量表(Nurses Obseration Scale for Inpatient Evaluation,NOSIE-30),分别在患者手语操治疗前和治疗后6周进行评定.结果 观察组经过6周的手语操音乐治疗干预后,SAS、SDS分值与对照组比较差异有统计意义(P<0.05),NOSIE-30中社会能力、社会兴趣、激惹、迟缓、抑郁5项因子分与对照组比较差异有统计意义(P<0.01).结论 手语操音乐疗法能改善男性服刑康复期精神分裂症患者的负性情绪,提高患者主动性、社会兴趣和社会能力,对心理康复有一定的帮助.

  14. Observations on the Therapeutic Effect of Combined Use of Acupuncture and Physiotherapy in the Early Convalescence of Cerebral Stroke Patients%针刺和理疗联合应用在脑卒中早期康复中的疗效观察

    Institute of Scientific and Technical Information of China (English)

    刘明; 任燕; 殷亚楠

    2009-01-01

    目的 观察针刺和理疗联合应用对中风患者运动能力及ADL的影响.方法 将300例急性脑卒中患者随机分为电针组、理疗组和针刺理疗联合组,各100例.电针组单纯采用针灸治疗,理疗组单纯采用脑超声中频电刺激治疗,针刺理疗联合组在脑超声中频电刺激的基础上加针刺治疗.比较三组功能改善情况,并对比三组的ADL能力.结果 治疗后,针刺理疗联合组的运动能力和ADL评分均高于电针组和理疗组(P<0.05).结论 针刺与理疗的联合疗效优于单纯针刺治疗和单纯理疗.

  15. 康复期精神分裂症患者幽默感与应对方式的相关研究%Correlation Study on the Sense of Humor and Coping Styles from Patients with Schizophrenia in Convalescence Stage

    Institute of Scientific and Technical Information of China (English)

    钟汉玲; 蔡春凤

    2012-01-01

    Objective: To investigate the relationship between the sense of humor and coping styles in patients with schizophrenia, and provide a basis for the development of the intervention plan with more pertinences. Methods-Ninety-four cases of schizophrenia in rehabilitation period received the survey by using multidimensional sense of humor scale, humorous style scale and stress coping style questionnaire. Results: (1) The scores of aggressive type of humor scale were statistically significant in patients with different gender (t = 3. 125, P<0. 05). The coping styles in 94 patients had no statistical difference with gender, age, course of disease and level of education. (2) The overall sense of humor had a positive correlation with the avoidant coping and coping task (r=0. 272, r = 0. 363, P< 0. 01). The affinitive humor had a negative correlation with emotional coping (r= -0. 230, P<0. 01). The aggressive humor had a positive correlation with emotional coping (r=0. 419, P<0. 01). Conclusion; The gender had effect in schizophrenic patients on aggressive type of humor. The patients with strong sense of humor were more likely to adopt avoidant coping and coping style. The patients good at using affinity humor occasionally used emotional coping style, and patients with aggressive humor more often used emotional coping style.%目的:探讨精神分裂症患者幽默感和应对方式的关系,为开发更有针对性干预方案提供依据.方法:运用多维度幽默感量表、幽默风格量表与压力情景应对方式问卷对94例精神分裂症康复期患者进行问卷调查.结果:①不同性别患者攻击性幽默类型亚量表得分有统计学意义(t=3.125,P<0.05);94例患者应对方式与性别、年龄、病程及文化程度差异均无统计学意义.②整体幽默感与回避应对、任务应对(r=0.272、r=0.363,P<0.01)成正相关;亲和幽默与情绪应对呈负相关(r=-0.230,P<0.01);攻击幽默与情绪应对成正相关(r=0.419,P<0.01).结论:性别对精神分裂症患者攻击幽默类型有影响;幽默感强的患者更可能采用回避应对与任务应对方式;擅长使用亲和幽默的患者更不经常使用情绪应对方式,而喜欢使用攻击幽默的患者更经常采用情绪应对方式.

  16. 自我效能干预对颅脑创伤恢复期患者康复训练效果的研究%Study of the Effect of Self-efficiency Interposition on Rehabilitation Training of Patients with Craniocerebral Trauma During Convalescence

    Institute of Scientific and Technical Information of China (English)

    洪秀英; 陈凌云; 金春燕

    2015-01-01

    目的:探讨自我效能干预对颅脑创伤恢复期患者康复训练的临床效果,寻求最有效的护理模式.方法:采用等组实验法将符合纳入、排除标准的108例患者,分为试验组和对照组,各54例.对照组予常规护理和康复训练,试验组增加自我效能的护理干预方法.干预前和干预3个月后分别对2组患者进行Karnofsky预后、基本生活活动能力(BADL)和辅助生活活动能力(IADL)评定.结果采用SPSS 17.0软件处理.结果:干预前2组患者Karnofsky预后、BADL和IADL评分比较差异均无统计学意义(P>0.05),干预3个月后试验组患者的Karnofsky预后、BADL和IADL评分差异均有统计学意义(P<0.01).结论:自我效能干预能提高颅脑创伤患者治病信心,增强对康复锻炼的坚持,改善肢体运动功能和日常生活能力.

  17. 奥拉西坦治疗重型脑伤恢复期病人记忆与智能障碍的临床研究%Clinical study of treating impediment of anamnesis and aptitude in the convalescent patients with severe head injury by oxiracetam

    Institute of Scientific and Technical Information of China (English)

    方波; 蔡云红; 袁鹏; 朱政鸣

    2005-01-01

    目的评价奥拉西坦(Oxiracetam)治疗重型脑伤(GCS3~8分)恢复期病人记忆与智能障碍的临床疗效.方法病例总数46例,按单、双号原则随机分为2组:对照组和治疗组各23例.运用简易精神状态量表(mini mental state examination,MMSE)测定治疗前和治疗90d后对照组与治疗组病人的分值.结果治疗组MMSE分值增加值明显高于对照组(P<0.05),具有统计学意义.结论奥拉西坦治疗治疗重型脑伤恢复期病人记忆与智能障碍有显著临床疗效.

  18. Pneumocystis carinii from pigs and humans are antigenically distinct

    DEFF Research Database (Denmark)

    Christensen, C B; Settnes, Osvald Peter; Bille-Hansen, Vivi;

    1996-01-01

    The antigens of Pneumocystis carinii cysts isolated from pigs and humans were compared by the Western immunoblotting technique. Convalescent pig serum reacted with two antigens (approximately 78 kDa and 32.5 kDa) of porcine P. carinii cysts, whereas convalescent serum from humans did not react wi...

  19. Prophylaxis against postoperative complications in gastroenterology

    DEFF Research Database (Denmark)

    Kehlet, H; Moesgaard, F

    1996-01-01

    Gastrointestinal surgery results in pain, profound endocrine metabolic changes and organ dysfunction, immunosuppression and decreased resistance to infection, fatigue and convalescence. The main pathogenetic mechanism is the surgical stress response, which may be reduced by minimal invasive...

  20. Akanmu et al., Afr J Tradit Complement Altern Med. (2011) 8(3):230 ...

    African Journals Online (AJOL)

    AJTCAM

    nutritive product for children, athletes and convalescents and health ... behaviors, learning and memory, hypnotic, anxiolytic, anticonvulsant and ...... pain transmission involves interplay of a number of neurotransmitters and ion channels.

  1. ISSN 2073-9990 East Cent. Afr. J. s 9990 East Cent. Afr. J. s

    African Journals Online (AJOL)

    Prof Kakande

    diffuse and result in paralytic or adhesive small bowel obstruction protracting convalescence and thus ... It is not possible to practice fully the ideal management of early diagnosis and .... Preventing infection after appendicectomy: a review.

  2. Travelers' Health: Typhoid and Paratyphoid Fever

    Science.gov (United States)

    ... or convalescent person or a chronic, asymptomatic carrier. Transmission through sexual contact, especially among men who have sex with ... fever even during visits of countries where the disease is highly endemic (such as India, Pakistan, or ...

  3. Prevention of postoperative ileus

    DEFF Research Database (Denmark)

    Holte, Kathrine; Kehlet, H

    2002-01-01

    Postoperative ileus (PI) is a major contributor to postoperative morbidity and prolonged convalescence after major surgical procedures. The pathophysiology of PI is multifactorial, including activation of the stress response to surgery, with inhibitory sympathetic visceral reflexes and inflammato...

  4. Treatment of postsurgical psychiatric complications

    National Research Council Canada - National Science Library

    Koch‐Stoecker, Steffi; Schmitz, Bettina; Kanner, Andres M

    2013-01-01

    ...) the coping phase during the first months after discharge; and (3) the reorientation phase. The early postoperative course is often dominated by physical problems that hamper success in convalescence...

  5. Erfaringer med laparoskopisk donornefrektomi på Herlev Hospital

    DEFF Research Database (Denmark)

    Boesby, Lene; Kromann-Andersen, Bjarne; Edwin, Bjørn

    2007-01-01

    INTRODUCTION: The purpose of this investigation was to evaluate the morbidity and convalescence of donors after laparoscopic nephrectomy. Transperitoneal laparoscopy was initiated at Herlev University Hospital in November 2002. MATERIALS AND METHODS: All 24 records from patients who underwent...

  6. 甲亢患者的内科治疗配合舆护理%Medical Therapy's Cooperation and Nursing of Hyperthyroidism

    Institute of Scientific and Technical Information of China (English)

    李柳芳; 陈彦茹; 戈兰; 陈琳玲

    2002-01-01

    To retrospect 46 cases of treating and nursing hyperthyroidism to sum up key point of therapy' s cooperation and nursing.The results suggests that strengthen the attention to antithyrotropic drugs. Psychological nursing and health education contribute to convalesce.

  7. Infectious Mononucleosis: Recognition and Management in Athletes.

    Science.gov (United States)

    Eichner, Edward R.

    1987-01-01

    Infectious mononucleosis strikes many young athletes. Considered here are its epidemiology, pathophysiology, diagnosis, natural course, complications, and management. The focus is on concerns of athletes with a perspective on personality, convalescence, and chronic fatigue. (Author/MT)

  8. Randomized clinical trial of dexamethasone versus placebo in laparoscopic inguinal hernia repair

    DEFF Research Database (Denmark)

    Tolver, M A; Strandfelt, P; Bryld, Clara E;

    2012-01-01

    The effect of dexamethasone on recovery and length of convalescence has not been evaluated in patients after laparoscopic groin hernia repair. It was hypothesized that preoperative intravenous dexamethasone would reduce postoperative pain....

  9. Multimodal approach to control postoperative pathophysiology and rehabilitation

    DEFF Research Database (Denmark)

    Kehlet, H

    1997-01-01

    Major surgery is still associated with undesirable sequelae such as pain, cardiopulmonary, infective and thromboembolic complications, cerebral dysfunction, nausea and gastrointestinal paralysis, fatigue and prolonged convalescence. The key pathogenic factor in postoperative morbidity, excluding...

  10. [DYNAMICS OF CYTOKINES IN INFANTS WITH ACUTE OBSTRUCTIVE BRONCHITIS AND THYMOMEGALIA].

    Science.gov (United States)

    Smiyan, O I; Plakhuta, V A; Bunda, T P; Popov, S V

    2015-01-01

    The article contains the information about pro- and anti-inflammatory cytokines in infants with acute obstructive bronchitis and thymus hypertrophy. The content of IL-6 and IL-10 has been de- termined in 101 children in acute period of disease and during early convalescence. It was found the increasing of production of proinflammatory IL-6 and a low level of anti-inflammatory IL-10 in the first days of illness in all children with thymomegalia. During convalescence in patients with acute obstructive bronchitis and thymomegalia IL-6 level was decreasing, and the level of IL-10 was in- creasing, but it did not amount to the level of healthy children.

  11. Sensorineural hearing loss in Lassa fever: two case reports

    Directory of Open Access Journals (Sweden)

    Okokhere Peter O

    2009-01-01

    Full Text Available Abstract Introduction Lassa fever is an acute arena viral haemorrhagic fever with varied neurological sequelae. Sensorineural hearing loss is one of the rare complications which occur usually during the convalescent stage of the infection. Case presentation The cases of two female patients aged 19 and 43 years old, respectively, with clinical features suggestive of Lassa fever and confirmed by immunoserological/Lassa-virus-specific reverse transcriptase polymerase chain reaction are presented. Both patients developed severe sensorineural hearing loss at acute phases of the infections. Conclusion Sensorineural hearing loss from Lassa fever infections can occur in both acute and convalescent stages and is probably induced by an immune response.

  12. Sex differences in laparoscopic cholecystectomy

    DEFF Research Database (Denmark)

    Thesbjerg, Simon E; Harboe, Kirstine Moll; Bardram, Linda

    2010-01-01

    Conversion from laparoscopic to open cholecystectomy may not be desirable due to the increased complication rate and prolonged convalescence. In Denmark, nationwide data show that 7.7% of the laparoscopic cholecystectomies are converted to open surgery. This article aims to document the relations...

  13. 38 CFR 4.44 - The bones.

    Science.gov (United States)

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false The bones. 4.44 Section 4... DISABILITIES Disability Ratings The Musculoskeletal System § 4.44 The bones. The osseous abnormalities incident... convalescence, and progress of recovery with development of permanent residuals. With shortening of a long...

  14. Production of mink enteritis parvovirus empty capsids by expression in a baculovirus vector system: a recombinant vaccine for mink enteritis parvovirus in mink

    DEFF Research Database (Denmark)

    Christensen, J; Alexandersen, Søren; Bloch, B.

    1994-01-01

    gene product was characterized after expression in Sf9 insect cells. The MEV VP-2 product had the same size as that reported for the wild-type MEV VP-2 protein and was recognized by convalescent sera from MEV-infected mink and a panel of monoclonal antibodies reactive to MEV. Furthermore, the VP-2...

  15. 38 CFR 51.2 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... day health care, and hospital care. Hospital care may be provided only when the State home also... convalescents or other persons who are not acutely ill and not in need of hospital care, but who require skilled... NURSING HOME CARE OF VETERANS IN STATE HOMES General § 51.2 Definitions. For purposes of this...

  16. Improving USAF Special Tactics Readiness to Meet the Operational Demands of the USAF and US Special Operations Command (SOCOM)

    Science.gov (United States)

    2002-06-01

    argued that due to the physical nature of the ST operator job, the instance of injury , and therefore, the amount of convalescence leave or non-available...TTR tracking system could be implemented to track progress. The TTR tracking could be used to 53 avoid overtraining specific events. The TTR

  17. Comparative analysis of salivary zinc level in recurrent herpes labialis

    Directory of Open Access Journals (Sweden)

    Faezeh Khozeimeh

    2012-01-01

    Conclusion: According to the results, zinc level is significantly lower in acute phase than in convalescent phase and significantly lower in both phases compared to healthy individuals,so determination of serum zinc level and prescribing zinc complement in low serum status has both treatmental and preventive effects in RHL patients.

  18. Guven et al., Afr., J. Infect. Dis.

    African Journals Online (AJOL)

    Convalescence-the recovery period- begins 10–20 days after the ... diagnosis for CCHF, as it is true for the many diseases, is performed by emergency services. ... In this study, we aimed to evaluated 200 cases with complaints of a tick bite ...

  19. Readmissions after fast-track hip and knee arthroplasty

    DEFF Research Database (Denmark)

    Husted, Henrik; Otte, Kristian Stahl; Kristensen, Billy B

    2010-01-01

    With the implementation of fast-track surgery with optimization of both logistical and clinical features, the postoperative convalescence has been reduced as functional milestones have been achieved earlier and consequently length of stay (LOS) in hospital has been reduced. However, it has been...

  20. 精神病易复发原因及对策%Cause and Countermeasure of Relapse of Psychosis

    Institute of Scientific and Technical Information of China (English)

    孙守宪; 韩瑛; 卢瑞美

    2002-01-01

    Objective To study the cause of relapse of psychosis for the practice of domestic nursing. Methods To analysizeretrospectively the 84 cases of psychosis relapse. Results The rate of relapse is high. (20.5 % in author's hospital). Conclusion To getrid of the social prejudice against the psychotics and care for them for their convalesce.

  1. Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood

    NARCIS (Netherlands)

    Tran, T.N.T.; de Vries, P.J.; Hoang, L.P.; Phan, G.T.; Le, H.Q.; Tran, B.Q.; Vo, C.M.T.; Nguyen, N.V.; Kager, P.A.; Nagelkerke, N.; Groen, J.

    2006-01-01

    Background: The reproducibilty of dengue IgM and IgG ELISA was studied in serum and filter paper blood spots from Vietnamese febrile patients. Methods: 781 pairs of acute (t0) and convalescent sera, obtained after three weeks (t3) and 161 corresponding pairs of filter paper blood spots were tested

  2. Comparison of two 3ABC enzyme-linked immunosorbent assays for diagnosis of multiple-serotype foot-and-mouth disease in a cattle population in an area of endemicity

    DEFF Research Database (Denmark)

    Bronsvoort, B.M.D.; Sørensen, K.J.; Anderson, J.;

    2004-01-01

    The development of a serological test for foot-and-mouth disease virus (FMDV) which is quick and easy to use, which can identify all seven serotypes, and which can differentiate vaccinated from convalescing or potential virus carriers would be a major advance in the epidemiological toolkit for FMDV...

  3. Celestin Freinet (1896-1966): A Most Unappreciated Educator in the Anglophone World.

    Science.gov (United States)

    Acker, Victor

    Celestin Freinet had a major influence on education in France. In 1917 he was critically injured during World War I and convalesced for two years. During World War II he was incarcerated by the Vichy government for his Communist tendencies, and, in the 1950s the French Communist Party harassed him for straying from their hard-line ideologies.…

  4. Program Outcomes and Soldier Perceptions of the Army Pregnancy and Postpartum Physical Training (P3T) Program

    Science.gov (United States)

    2014-07-24

    duty work force each year to pregnancy-related complications, childbirth , and convalescent leave is potentially burdensome to the Army’s resources (3...1982. Self-efficacy mechanism in human agency. American Psychologist 37:122-47 10. Beilock SL, Feltz DL, Pivarnik JM. 2001. Training patterns of

  5. FY2017 National Defense Authorization Act: Selected Military Personnel Issues

    Science.gov (United States)

    2016-07-29

    maternity leave as, “a convalescent period up to 6 weeks following pregnancy and childbirth .” The new policy, as announced, extended the period of...Authorization Act: Selected Military Personnel Issues Congressional Research Service 44 Title Summary clandestine human intelligence collection

  6. INFLUENCE PRODUCED BY THE URBAN INFRASTRUCTURE DEVELOPMENT ON THE LIVING ENVIRONMENT

    Directory of Open Access Journals (Sweden)

    Giyasov Botir Iminzhonovich

    2012-07-01

    There is an urgent need to improve the architectural and planning patterns of urban territories that need hygienic regulations applicable both to residential and industrial areas, highways, parks, office buildings, leisure and community service buildings, schools, hospitals, convalescence houses, kindergartens, etc.

  7. Clinical outcome of critically ill, not fully recompensated, patients undergoing MitraClip therapy

    DEFF Research Database (Denmark)

    Rudolph, Volker; Huntgeburth, Michael; von Bardeleben, Ralph Stephan

    2014-01-01

    AIMS: As periprocedural risk is low, MitraClip implantation is often performed in critically ill, not fully recompensated patients, who are in NYHA functional class IV at the time of the procedure, to accelerate convalescence. We herein sought to evaluate the procedural and 30-day outcome of this...

  8. The preoperative preparation and postoperative care of interventional treatment for coronary heart disease%冠心病介入治疗的术前准备和术后处理

    Institute of Scientific and Technical Information of China (English)

    韩雅玲

    2002-01-01

    The well performance of everything in the peripheral operative period is important to improve successful rate, decrease complications and assure favorable convalescence of patients for interventional treatment of coronary heart disease. It is mentioned that the primary management of peripheral operative period and main notes for interventional treatment of coronary heart disease in the paper summarily.

  9. Sex differences in laparoscopic cholecystectomy

    DEFF Research Database (Denmark)

    Thesbjerg, Simon E; Harboe, Kirstine Moll; Bardram, Linda

    2010-01-01

    Conversion from laparoscopic to open cholecystectomy may not be desirable due to the increased complication rate and prolonged convalescence. In Denmark, nationwide data show that 7.7% of the laparoscopic cholecystectomies are converted to open surgery. This article aims to document...... the relationship of gender to conversion rate and length of hospital stay after laparoscopic cholecystectomy in a national cohort of patients....

  10. 42 CFR 436.1005 - Institutionalized individuals.

    Science.gov (United States)

    2010-10-01

    ... (2) Individuals under age 65 who are patients in an institution for mental diseases unless they are... for mental diseases. (c) An individual on conditional release or convalescent leave from an institution for mental diseases is not considered to be a patient in that institution. However, such...

  11. 42 CFR 435.1009 - Institutionalized individuals.

    Science.gov (United States)

    2010-10-01

    ... are patients in an institution for mental diseases unless they are under age 22 and are receiving... inmate of a public institution or a patient in an institution for tuberculosis or mental diseases. (c) An individual on conditional release or convalescent leave from an institution for mental diseases is...

  12. Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood

    NARCIS (Netherlands)

    T.N.T. Tran (Thanh Nga); P.J. de Vries (Peter); L.P. Hoang (Lan Phuong); G.T. Phan (Giao); H.Q. Le (Hung); B.Q. Tran (Binh); C.M.T. Vo (Chi Mai); N.V. Nguyen (Nam); P.A. Kager (Piet); N.J.D. Nagelkerke (Nico); J.M. Groen (Jan)

    2006-01-01

    textabstractBackground: The reproducibilty of dengue IgM and IgG ELISA was studied in serum and filter paper blood spots from Vietnamese febrile patients. Methods: 781 pairs of acute (t0) and convalescent sera, obtained after three weeks (t3) and 161 corresponding pairs of filter paper blood spots

  13. Medical Assistant. [FasTrak Specialization Integrated Technical and Academic Competency (ITAC).] 2002 Revision.

    Science.gov (United States)

    Ohio State Dept. of Education, Columbus. Div. of Career-Technical and Adult Education.

    This curriculum for a medical assistant program is designed for students interested in caring for the sick, injured, convalescent, or disabled under the direction of the family, physicians, and credentialed nurses. The curriculum is divided into 12 units: orientation to medical assisting; principles of medical ethics; risk management; infection…

  14. Isolation of Clostridium tetani from anaerobic empyema.

    Science.gov (United States)

    Mayall, B C; Snashall, E A; Peel, M M

    1998-11-01

    We report the isolation of Clostridium tetani (along with Fusobacterium mortiferum) from empyema pus. The patient, a 68 year old retired farmer from rural NSW, had recently undergone cholecystectomy, had heart failure and developed an empyema. He improved after drainage of the empyema and penicillin therapy, but died suddenly during convalescence.

  15. 20 CFR Appendix 1 to Subpart P of... - Listing of Impairments

    Science.gov (United States)

    2010-04-01

    .... Part A Criteria applicable to individuals age 18 and over and to children under age 18 where criteria..., including burns, requiring prolonged periods of immobility or convalescence. The provisions of 1.02 and 1.03... one's home without the use of assistive devices does not, in and of itself, constitute...

  16. Fast-track revision knee arthroplasty

    DEFF Research Database (Denmark)

    Husted, Henrik; Otte, Niels Kristian Stahl; Kristensen, Billy B;

    2011-01-01

    Abstract Background and purpose Fast-track surgery has reduced the length of hospital stay (LOS), morbidity, and convalescence in primary hip and knee arthroplasty (TKA). We assessed whether patients undergoing revision TKA for non-septic indications might also benefit from fast-track surgery...

  17. MANPOWER FOR CALIFORNIA HOSPITALS, 1964-1975.

    Science.gov (United States)

    California State Commission on Manpower, Automation, and Technology, Sacramento.

    AN EXAMINATION OF THE HOSPITAL AND NURSING AND CONVALESCENT HOME INDUSTRY IN 1964 AND EMPLOYMENT PROJECTIONS ARE PRESENTED AS AN INITIAL CONTRIBUTION TO THE DEVELOPMENT OF AN ONGOING MANPOWER INFORMATION PROGRAM IN THE STATE. DATA WERE COMPILED FROM POPULATION PROJECTIONS BY THE CALIFORNIA DEPARTMENT OF FINANCE, WAGE SURVEY STUDIES BY THE…

  18. Scope of a nursing diagnostic list for fulfilling basic human needs in home-visit nursing.

    Science.gov (United States)

    Esaki, Fusako; Muranaka, Yoko; Tamaki, Miyoko; Akiba, Kimiko; Aoki, Ryouko

    2006-01-01

    Inscriptions on 291 nursing diagnosis items regarding 100 home convalescents were subjected to analysis. The 291-item nursing diagnosis was found to be consistent with a previously reported nursing diagnosis developed by the authors from Henderson's 14 items covering basic nursing practice. Additionally, the analysis indicated a need for nursing diagnosis items for family members, as caregivers for the patients recuperating at home.

  19. Intensive care diaries reduce new onset post traumatic stress disorder following critical illness

    DEFF Research Database (Denmark)

    Jones, Christina; Bäckman, Carl; Capuzzo, Maurizia

    2010-01-01

    Patients recovering from critical illness have been shown to be at risk of developing Post Traumatic Stress disorder (PTSD). This study was to evaluate whether a prospectively collected diary of a patient's intensive care unit (ICU) stay when used during convalescence following critical illness...

  20. Leukotriene pathway polymorphisms are associated with altered cysteinyl leukotriene production in children with acute asthma.

    Science.gov (United States)

    Bizzintino, Joelene A; Khoo, Siew-Kim; Zhang, Guicheng; Martin, Andrew C; Rueter, Kristina; Geelhoed, Gary C; Goldblatt, Jack; Laing, Ingrid A; Le Souëf, Peter N; Hayden, Catherine M

    2009-07-01

    Cysteinyl leukotrienes (cysLTs) are pro-inflammatory mediators with increasing evidence for a role in childhood acute asthma. This study examined the influence of polymorphisms in cysLT pathway genes on urinary leukotriene E(4) (uLTE(4)) levels and clinical status in acute asthmatic children. Children aged 2-16 years were recruited during an asthma attack (n=205). Where possible, asthma severity scores were assigned, ALOX5AP G-336A, ALOX5 G-1708A, LTC4S A-444C and G-1072A, GPX4 C718T, and CYSTLTR1 T927C genotypes were determined and uLTE(4) was measured in acute and convalescent samples. uLTE(4) levels were higher acutely compared with convalescence (acute GM: 115.7pg/mg creatinine; 95% CI 88.6-151.1, convalescence GM: 66.4pg/mg creatinine; 95% CI 51.5-85.6; n=50 paired samples, p=0.003) and paired sample analysis showed genotype-specific effects with significantly increased uLTE(4) for LTC(4)S-444AA (acute GM: 127.9pg/mg creatinine; 95% CI 91.8-178.3, convalescence GM: 68.2pg/mg creatinine; 95% CI 50.5-92.0; n=32, p=0.002), LTC(4)S-1072 GG (acute GM: 126.7pg/mg creatinine; 95% CI 95.4-168.3, convalescence GM: 78.9pg/mg creatinine; 95% CI 59.7-104.1; n=39, p=0.019) and CYSLTR1 927 TT/T_ (acute GM: 96.8pg/mg creatinine; 95% CI 73.8-126.9, convalescence GM: 62.4pg/mg creatinine; 95% CI 46.8-83.3; n=28, p=0.036) but not AC/CC, GA/AA, or TC/CC/C_, respectively. When we compared the allele frequencies of the CYSLTR1 SNP between asthmatics and non-asthmatics, the 927C allele was found to be a risk allele for asthma (OR=2.13, 95% CI: 1.06-4.26, p=0.033). Genotypes were not associated with acute or convalescent uLTE(4) levels alone and neither the SNPs nor uLTE(4) correlated with acute asthma severity. Leukotriene pathway gene polymorphisms may influence the magnitude of cysLT production during an attack, yet their influence alone may not be substantial enough to alter the severity of exacerbations.

  1. Potential Impact of Sexual Transmission on Ebola Virus Epidemiology: Sierra Leone as a Case Study.

    Directory of Open Access Journals (Sweden)

    Jessica L Abbate

    2016-05-01

    Full Text Available Sexual transmission of Ebola virus disease (EVD 6 months after onset of symptoms has been recently documented, and Ebola virus RNA has been detected in semen of survivors up to 9 months after onset of symptoms. As countries affected by the 2013-2015 epidemic in West Africa, by far the largest to date, are declared free of Ebola virus disease (EVD, it remains unclear what threat is posed by rare sexual transmission events that could arise from survivors.We devised a compartmental mathematical model that includes sexual transmission from convalescent survivors: a SEICR (susceptible-exposed-infectious-convalescent-recovered transmission model. We fitted the model to weekly incidence of EVD cases from the 2014-2015 epidemic in Sierra Leone. Sensitivity analyses and Monte Carlo simulations showed that a 0.1% per sex act transmission probability and a 3-month convalescent period (the two key unknown parameters of sexual transmission create very few additional cases, but would extend the epidemic by 83 days [95% CI: 68-98 days] (p < 0.0001 on average. Strikingly, a 6-month convalescent period extended the average epidemic by 540 days (95% CI: 508-572 days, doubling the current length, despite an insignificant rise in the number of new cases generated.Our results show that reductions in the per sex act transmission probability via abstinence and condom use should reduce the number of sporadic sexual transmission events, but will not significantly reduce the epidemic size and may only minimally shorten the length of time the public health community must maintain response preparedness. While the number of infectious survivors is expected to greatly decline over the coming months, our results show that transmission events may still be expected for quite some time as each event results in a new potential cluster of non-sexual transmission. Precise measurement of the convalescent period is thus important for planning ongoing surveillance efforts.

  2. Postinfectious syndrome of convalescentsixodes tick-borne borreliosis

    Directory of Open Access Journals (Sweden)

    O. N. Sumlivaya

    2014-01-01

    Full Text Available The aim: to study the psycho-vegetative status of the quantitative contents of serotonin in blood platelets in patients after the Ixodes tick-borne borreliosis, to evaluate the clinical efficiency treatment by adamantilfenilamin of postinfection asthenia.Materials and methods: there was clinical supervision and inspection conducted of 118 convalescents borreliosis after a course of inpatient treatment. All patients were examined using psychovegetative tests. Platelet serotonin concentration levels were measured by enzyme immunoassay. For the treatment of postinfectious asthenia 36 convalescents received adamantilbromfenilamin in a dose of 100 mg for 25 days.Results: when tested convalescents marked change in indicators of emotional and personality disorders. Quantitative study of blood platelet serotonin content revealed a significant decrease in this indicator relative to control values. Study the correlations between obtained when testing the psycho-emotional parameters and platelet serotonin levels showed a negative correlation between serotonin and an indicator of reactive anxiety (R = -0,81, p <0,05. To correct these violations convalescents with severe asthenia postinfection were treated adamantilfenilamin. Established clinical efficacy contributing to the improvement of the quality of life.Conclusion: the research of neurotransmitter serotonin in patients during the convalescence period after borreliosis possible to evaluate the extent of potential damage to the nervous tissue in the inflammatory process and its involvement in the formation of anxiety and depressive symptoms. adamantilbromfenilamin can be recommended for rehabilitation patients with residual effects in the form of postinfectious asthenia.

  3. Clinical significance of late high-degree atrioventricular block in patients with left ventricular dysfunction after an acute myocardial infarction--a Cardiac Arrhythmias and Risk Stratification After Acute Myocardial Infarction (CARISMA) substudy

    DEFF Research Database (Denmark)

    Gang, Uffe Jakob Ortved; Jøns, Christian; Jørgensen, Rikke Mørch;

    2011-01-01

    High-degree atrioventricular block (HAVB) is a frequent complication in the acute stages of a myocardial infarction associated with an increased rate of mortality. However, the incidence and clinical significance of HAVB in late convalescent phases of an AMI is largely unknown. The aim of this st......High-degree atrioventricular block (HAVB) is a frequent complication in the acute stages of a myocardial infarction associated with an increased rate of mortality. However, the incidence and clinical significance of HAVB in late convalescent phases of an AMI is largely unknown. The aim...... of this study was to assess the incidence and prognostic value of late HAVB documented by continuous electrocardiogram (ECG) monitoring in post-AMI patients with reduced left ventricular function....

  4. Cytokine Profiles in Malawian Children Presenting with Uncomplicated Malaria, Severe Malarial Anemia, and Cerebral Malaria.

    Science.gov (United States)

    Mandala, Wilson L; Msefula, Chisomo L; Gondwe, Esther N; Drayson, Mark T; Molyneux, Malcolm E; MacLennan, Calman A

    2017-04-01

    Proinflammatory cytokines are involved in clearance of Plasmodium falciparum, and very high levels of these cytokines have been implicated in the pathogenesis of severe malaria. In order to determine how cytokines vary with disease severity and syndrome, we enrolled Malawian children presenting with cerebral malaria (CM), severe malarial anemia (SMA), and uncomplicated malaria (UCM) and healthy controls. We analyzed serum cytokine concentrations in acute infection and in convalescence. With the exception of interleukin 5 (IL-5), cytokine concentrations were highest in acute CM, followed by SMA, and were only mildly elevated in UCM. Cytokine concentrations had fallen to control levels when remeasured at 1 month of convalescence in all three clinical malaria groups. Ratios of IL-10 to tumor necrosis factor alpha (TNF-α) and of IL-10 to IL-6 followed a similar pattern. Children presenting with acute CM had significantly higher concentrations of TNF-α (P Mandala et al.

  5. Procedure-specific pain management and outcome strategies

    DEFF Research Database (Denmark)

    Joshi, Girish P; Schug, Stephan A; Kehlet, Henrik

    2014-01-01

    Optimal dynamic pain relief is a prerequisite for optimizing post-operative recovery and reducing morbidity and convalescence. Procedure-specific pain management initiative aims to overcome the limitations of conventional guidelines and provide health-care professionals with practical recommendat......Optimal dynamic pain relief is a prerequisite for optimizing post-operative recovery and reducing morbidity and convalescence. Procedure-specific pain management initiative aims to overcome the limitations of conventional guidelines and provide health-care professionals with practical....... There is emphasis on the use of multimodal analgesia and preventive analgesia aimed at reducing central sensitization. Importantly, the benefits of dynamic pain relief may only be realized if other aspects of perioperative care such as the use of minimally invasive surgery, approaches to reduce stress responses......, optimizing fluid therapy and optimizing post-operative nursing care with early mobilization and oral feeding are utilized....

  6. Seduction and the hospitalized person.

    Science.gov (United States)

    Whitley, M P

    1978-06-01

    Seductive behavior seen in the convalescent individual is generally a signal that the patient is in the process of assessment. It is not a social invitation to sexual activity. The patient is merely trying to reassess his situation after having experienced an illness, injury or surgery. Nurses have an obligation to be informed regarding the effects of illness on sexuality, and an obligation to recognize the patient's seductive signals. Nurses need to recognize the mechanics of seductive behavior and be able to qualify the behavior in order to help the patient express his/her concerns during convalescence. Finally, nurses have an obligation to discover or create referral sources in their local area where patients may seek help with their altered sexual function, should that be warranted.

  7. Psychological impact of the diagnosis of breast cancer on the patient and her family.

    Science.gov (United States)

    Northouse, L L

    1992-01-01

    The diagnosis of breast cancer creates emotional distress for patients as well as family members. This article reviews studies on the psychological adjustment of women and their family members during the diagnosis, hospitalization, and early convalescence from breast surgery. Studies indicate that the diagnostic phase is an extremely stressful time for women, marked by high anxiety, uncertainty, and difficulty making decisions. The hospital phase is especially difficult for spouses, who must juggle work responsibilities with added home responsibilities and also spend time at the hospital supporting their wives. In the convalescent phase, patients and family members need to adjust to changes in family roles, cope with fears about recurrence, and learn to balance the needs of all family members. In order to provide high quality health care to breast cancer patients and their family members, physicians and nurses need to address the emotional as well as the physical aspects of recovery.

  8. Inflammatory Responses to Salmonella Infections Are Serotype-Specific

    Directory of Open Access Journals (Sweden)

    Zhanna Ktsoyan

    2013-01-01

    Full Text Available The main purpose of this study was to investigate the profile of inflammatory response in patients with acute salmonellosis caused by two serotypes of Salmonella enterica, S. Enteritidis and S. Typhimurium, as well as in convalescent patients with previous acute disease caused by S. Enteritidis. Patients with acute disease showed significantly elevated levels of IL-1β, IL-17, IL-10, and calprotectin compared to healthy control subjects. In convalescent patients, these markers were also significantly elevated, with the exception of IL-1β. Multivariate statistical analyses with the use of these variables produced models with a good predictive accuracy resulting in excellent separation of the diseased and healthy cohorts studied. Overall, the results suggest that the profile of inflammatory response in this disease is determined, to a significant degree, by the serotype of Salmonella, and the profile of certain cytokines and calprotectin remains abnormal for a number of months following the acute disease stage.

  9. IL-18 and IL-18 binding protein levels in patients with dengue virus infection

    Institute of Scientific and Technical Information of China (English)

    Chintana Chirathaworn; Yong Poovorawan; Viboonsak Vuthitanachot

    2010-01-01

    Objective:To determine the levels ofIL-18 andIL-18binding protein(BP) in patients with dengue virus infection.Methods: Acute and convalescent sera were collected from each patient. Control group was sera from blood donors. The levels of both IL-18 andIL-18BP were measured byELISA assays.Results: It was shown thatIL-18 andIL-18BPlevels were significantly higher in patients when compared with controls. In addition, the level ofIL-18BP was lower in convalescent than in acute sera.Conclusions: These data suggest that bothIL-18 andIL-18BP production was induced following dengue virus infection. Investigating the regulation of IL-18 by its natural regulator could lead to further understanding of the immune response or immunopathogenesis following dengue virus infection.

  10. В ЭКСПЕРИМЕНТЕ

    Directory of Open Access Journals (Sweden)

    Бондарь, С.С.

    2016-04-01

    Full Text Available The study discusses the state of the immune regulation and the ways of its correction in military, patients with community-acquired pneumonia in the stage of convalescence. In examined patients in comparison with healthy individuals revealed increased spontaneous production by whole blood cells interleukins IL-2, IL-10, IFN-?. In convalescents also revealed a significant increase in mitogen-stimulated production of cytokines and reduced phagocytic function of the cells. Microwave radiation at a frequency of 1 GHz accompanied by a normalization of activity of phagocytes of whole blood with the decline of cells to produce IL-10 and enhancing IL-2. In relation to stimulated cells in whole blood, the microwaves increase the production of IL-10 and decrease IL-2.

  11. Effect of physical therapy frequency on gross motor function in children with cerebral palsy

    OpenAIRE

    Park, Eun-Young

    2016-01-01

    [Purpose] This study attempted to investigate the effect of physical therapy frequency based on neurodevelopmental therapy on gross motor function in children with cerebral palsy. [Subjects and Methods] The study sample included 161 children with cerebral palsy who attended a convalescent or rehabilitation center for disabled individuals or a special school for children with physical disabilities in South Korea. Gross Motor Function Measure data were collected according to physical therapy fr...

  12. Assessment of DoD Wounded Warrior Matters -- Camp Lejeune

    Science.gov (United States)

    2012-03-30

    people who are sick, injured, convalescent, or disabled under the direction of physicians and registered nurses. The nature of the direction and...bicycle, swim, or kayak , etc. This is not reality.” He added that by making the WAR program mandatory, the battalion was creating a counter...contradictory. “It says don’t PT on your profile, but the battalion expects you to row a kayak .” • A Warrior added that he was concerned about

  13. Uncoupling GP1 and GP2 Expression in the Lassa Virus Glycoprotein Complex: Implications for GP1 Ectodomain Shedding

    Science.gov (United States)

    2008-12-23

    contributors Abstract Background: Sera from convalescent Lassa fever patients often contains antibodies to Lassa virus (LASV) glycoprotein 1 (GP1...uncoupled proteins will improve current diagnostics, treatment, and prevention of Lassa fever . To this end, mammalian expression systems were engineered for...stages of Lassa fever . Published: 23 December 2008 Virology Journal 2008, 5:161 doi:10.1186/1743-422X-5-161 Received: 14 December 2008 Accepted: 23

  14. Uncoupling GP1 and GP2 Expression in the Lassa Virus Glycoprotein Complex: Implications for GPI Ectodomain Shedding

    Science.gov (United States)

    2008-12-23

    contributors Abstract Background: Sera from convalescent Lassa fever patients often contains antibodies to Lassa virus (LASV) glycoprotein 1 (GP1...uncoupled proteins will improve current diagnostics, treatment, and prevention of Lassa fever . To this end, mammalian expression systems were engineered for...stages of Lassa fever . Published: 23 December 2008 Virology Journal 2008, 5:161 doi:10.1186/1743-422X-5-161 Received: 14 December 2008 Accepted: 23

  15. Epidural anaesthesia and analgesia - effects on surgical stress responses and implications for postoperative nutrition

    DEFF Research Database (Denmark)

    Holte, Kathrine; Kehlet, H

    2002-01-01

    for improved mobilization and oral nutrition, and preservation of body composition and muscle function. Studies integrating continuous epidural local anaesthetics with enforced early nutrition and mobilization uniformly suggest an improved recovery, decreased hospital stay and convalescence. CONCLUSIONS......: Epidural local anaesthetics should be included in a multi-modal rehabilitation programme after major surgical procedures in order to facilitate oral nutrition, improve recovery and reduce morbidity....

  16. Clinical significance of late high-degree atrioventricular block in patients with left ventricular dysfunction after an acute myocardial infarction--a Cardiac Arrhythmias and Risk Stratification After Acute Myocardial Infarction (CARISMA) substudy

    DEFF Research Database (Denmark)

    Gang, Uffe Jakob Ortved; Jøns, Christian; Jørgensen, Rikke Mørch;

    2011-01-01

    High-degree atrioventricular block (HAVB) is a frequent complication in the acute stages of a myocardial infarction associated with an increased rate of mortality. However, the incidence and clinical significance of HAVB in late convalescent phases of an AMI is largely unknown. The aim of this st...... of this study was to assess the incidence and prognostic value of late HAVB documented by continuous electrocardiogram (ECG) monitoring in post-AMI patients with reduced left ventricular function....

  17. [Laparoscopic adrenalectomy. A general review of a current technic].

    Science.gov (United States)

    Joual, A

    1998-01-01

    The anatomical situation of the adrenal gland explains the diversity of the surgical incisions used for adrenalectomy. Laparoscopic adrenalectomy was first performed in 1992. It allows eradication of small adrenal lesions, which are often incidental findings. There are two routes of laparoscopic access to the adrenal gland: transperitoneal and retroperitoneal. Laparoscopy allows excellent operative exposure, reduction of postoperative pain and hospital stay and rapid convalescence. Retroperitoneoscopy, by allowing direct access to the retroperitoneum, is more rapid than the transperitoneal route.

  18. [The implantation of bipolar coagulation to remove endometriosis foci].

    Science.gov (United States)

    Sobkiewicz, S; Palatyński, A; Salata, I

    2001-05-01

    The paper compares the results of bipolar coagulation bey means of ERBE ICC 300 diatermy coagulator and WISAP endocoagulator. The results of both types of coagulation were assessed with reference to the changes occurring on peritoneum ligamenti sacro-uterini, Douglas pouch and ovary. The best results of endometriosis foci coagulation were obtained with bipolar ball at 20-30 W; no side effects or feelings of malaise were observed in patients just after the operation or over a longer period of convalescence.

  19. Reduced length of stay following hip and knee arthroplasty in Denmark 2000-2009: from research to implementation

    DEFF Research Database (Denmark)

    Husted, Henrik; Jensen, Claus Munk; Solgaard, Søren

    2012-01-01

    Fast-track surgery is the combination of optimized clinical and organizational factors aiming at reducing convalescence and perioperative morbidity including the functional recovery resulting in reduced hospitalization. As the previous nationwide studies have demonstrated substantial variations...... in length of stay (LOS) following standardized operations such as total hip and knee arthroplasty (THA and TKA), this nationwide study was undertaken to evaluate the implementation process of fast-track THA and TKA in Denmark....

  20. Bilateral microvascular second toe transfer for bilateral post-traumatic thumb amputation

    OpenAIRE

    Rajendra Nehete; Anita Nehete; Sandeep Singla; Harshad Adhav

    2012-01-01

    In bilateral thumb amputations, the functional impairment is serious and every attempt should be made to reconstruct the thumb. We report a case of bilateral post traumatic thumb amputation, reconstructed with bilateral second toe transfer. Only two such cases have been reported in literature so far. Though there are various modalities for the reconstruction of thumb, microvascular toe transfer has its own merits. The convalescent period is minimal with excellent function. It is bilaterally s...

  1. A Prospective Observational Study of Abdominal Injury Management in Contemporary Military Operations: Damage Control Laparotomy Is Associated With High Survivability and Low Rates of Fecal Diversion

    Science.gov (United States)

    2015-04-01

    extra-abdominal complications, time in the operating theatre, intensive care unit ( ICU ) and hospital lengths of stay, and mortality. We defined a DCL as...4” provides “definitive care . . . [comprising] specialist surgical proce- dures, reconstruction, rehabilitation , and convalescence. This level of...as the time at which abdominal activity ended was not recorded. Data on operating theatre time and ICU stay relate only to activity at Role 3, as we

  2. FY2017 National Defense Authorization Act: Selected Military Personnel Issues

    Science.gov (United States)

    2017-01-23

    Existing DOD policy defined maternity leave as, “a convalescent period up to 6 weeks following pregnancy and childbirth.” The new policy, as...discharge review boards of claims asserting Post- Traumatic Stress Disorder (PTSD) or Traumatic Brain Injury (TBI) in connection with sexual trauma...535 requires additional consideration by discharge review boards of claims asserting Post- Traumatic Stress Disorder (PTSD) or Traumatic Brain

  3. Advertisement Scheduling in Television Channels: An Application of Mixed Integer Programming

    OpenAIRE

    ULUCAN, Aydın; Ülker, Onur

    2005-01-01

    In recent years, the increment in the number of TV channels, increased the digital broadcasting and wired homes. Developing of convalescence, television advertising has become much more important, commercial companies allocate their advertisement budgets on TV ads. TV channels need professional advertisement scheduling techniques in order to increase spot sales profitability and productivities. As per date, many methods have been evaluated in spot scheduling. The most important ones are, ‘int...

  4. Difference between early versus delayed postoperative physical rehabilitation protocol following arthroscopic rotator cuff repair

    Directory of Open Access Journals (Sweden)

    Samar M Fawzy

    2016-01-01

    Conclusion Significant improvement in pain, ROM, and function after arthroscopic rotator cuff repair was seen at 1 year postoperatively, regardless of early or delayed postoperative rehabilitation protocols. However, early motion increases pain scores and may increase the possibility of rotator cuff retear but with early regain of ROM. A delayed rehabilitation protocol with immobilization for 6 weeks would be better for tendon healing without risk for retear or joint stiffness and easily convalescence with less postoperative pain.

  5. Prevalence of hearing problems, and use of hearing aids among a sample of elderly patients.

    OpenAIRE

    Liston, R; Solomon, S.; Banerjee, A K

    1995-01-01

    BACKGROUND. Deterioration of hearing with advancing age is well documented. However, the proportion of elderly people with hearing problems who wear hearing aids is low. AIM. The aim of this study was to assess the prevalence of hearing disability in a group of elderly patients in hospital and to determine their attitudes to hearing difficulties and the wearing of hearing aids. METHOD. A random sample of patients who were convalescing were interviewed. A detailed questionnaire was administere...

  6. [Complications of purulent meningoencephalitis in children].

    Science.gov (United States)

    Rubin, A N; Shcherbuk, Yu A; Lyapin, A P

    2015-01-01

    An analysis of 19 cases of meningoencephalitis was made in infants aged under one year old. The disease was complicated by chronic subdural hematomas in 11 patients and by hydrocephalus in 8 patients. The article presents the strategy, treatment results and diagnostic procedures volume. Based on their work, the authors made a conclusion that meningoencephalitis required an emergency neurosurgical interference in order to avoid complications in convalescence period.

  7. Studies on the Antigenic Composition of Coxiella Burnetii.

    Science.gov (United States)

    1981-12-01

    able to evaluate the development of cell-mediated inmunity to C. burnetli during the acute and convalescent phase of Q-fever that occurred in a...obtained from C. burnetit immune mice in standard passive transfer assays. We * e mployed normaT and nude mice as recipients of these immunologic...C. burnetii was continuing unchecked. Effects of Antibody in C. burneti Infection as Determined by Passive Transfer The role of antibody in

  8. Studies on Typhus and Spotted Fever

    Science.gov (United States)

    1979-02-01

    demonstrated by passive transfer experiments the salient role played by splenic lymphocytes from immune guinea pigs, in contrast to inmune serum, in...into the cytoplasm where they grew and eventually des- troyed the macrophages. Immune serum passively transferred to mice protected them against death...McGregor (30) for Listeria. Passive transfer of large quantities of convalescent guinea pig serum to non-immune recipients failed to control the

  9. Evaluation of a new latex agglutination test for detection of streptolysin O antibodies.

    OpenAIRE

    Gerber, M. A.; Caparas, L S; Randolph, M F

    1990-01-01

    Acute- and convalescent-phase serum specimens were collected from 50 patients with group A streptococcal pharyngitis. The anti-streptolysin O (ASO) titer for each serum specimen was determined by using both the standard neutralization assay and the latex agglutination (LA) test (Rheumagen ASO; Biokit Inc., New Britain, Conn.). When the ASO titers derived by the two methods were compared, the correlation coefficient was 0.93. When the ability of the LA test to demonstrate a significant ASO tit...

  10. Sickle cell trait is associated with controlled levels of haem and mild proinflammatory response during acute malaria infection

    Science.gov (United States)

    Ademolue, T. W.; Amodu, O. K.

    2017-01-01

    Summary The controlled induction of haemoxygenase‐1 (HO‐1), an enzyme that catabolizes haem, has been shown to reduce haem, preventing pathologies associated with haem toxicity. The hemoglobin genotype HbAS confers reduced susceptibility to severe complications of malaria by a mechanism that is not well understood. Using a longitudinal approach, we investigated the effect of baseline concentrations of HO‐1 on the accumulation of haem during acute Plasmodium falciparum malaria in HbAS and HbAA genotypes. Plasma concentrations of haem, HO‐1 and cytokines were quantified in venous blood obtained from children (9 months–5 years of age) during malaria infection, and at convalescence (baseline levels). Parasitaemia was determined during malaria infection. In patients with the HbAA genotype, there was a significant elevation in the plasma concentration of haem (P = 0.002), and a consequent increased induction of HO‐1 (P < 0.001) during falciparum malaria compared with levels at convalescence. Contrary to HbAA, plasma concentration of haem did not change in the HbAS genotypical group (P = 0·110), and the induction of HO‐1 was reduced during malaria compared with levels at convalescence (P = 0·006). Higher plasma levels of haem were observed in HbAS compared with HbAA at convalescence (P = 0·010), but this difference did not affect the levels of HO‐1 within each genotype (P = 0·450). Relatively milder proinflammatory responses were observed in HbAS children during malaria infection compared to HbAA children. Our findings suggest that a mechanism of reduced susceptibility to severe malaria pathologies by the HbAS genotype may involve the control of haem, leading to controlled levels of HO‐1 and milder proinflammatory responses during acute malaria. PMID:28142190

  11. Generation and Characterization of Protective Antibodies to Marburg Virus

    Science.gov (United States)

    2017-04-03

    utility of antibody passive transfer therapies in NHP models of filovirus infections [14]. EBOV- or MARV- infected NHPs were fully protected when treated...with immunoglobulin G purified from species-matched convalescent serum, even when treatment was delayed 48 hours post- infection . The first...Sullivan, N.J., et al., Development of a preventive vaccine for Ebola virus infection in primates. Nature, 2000. 408(6812): p. 605-9. 10. Swenson

  12. Correlation between dengue-specific neutralizing antibodies and serum avidity in primary and secondary dengue virus 3 natural infections in humans.

    Directory of Open Access Journals (Sweden)

    Andreas Puschnik

    Full Text Available Although heterotypic secondary infection with dengue virus (DENV is associated with severe disease, the majority of secondary infections are mild or asymptomatic. The mechanisms of antibody-mediated protection are poorly understood. In 2010, 108 DENV3-positive cases were enrolled in a pediatric hospital-based study in Managua, Nicaragua, with 61 primary and 47 secondary infections. We analyzed DENV-specific neutralization titers (NT50, IgM and IgG avidity, and antibody titer in serum samples collected during acute and convalescent phases and 3, 6, and 18 months post-infection. NT50 titers peaked at convalescence and decreased thereafter. IgG avidity to DENV3 significantly increased between convalescent and 3-month time-points in primary DENV infections and between the acute and convalescent phase in secondary DENV infections. While avidity to DENV2, a likely previous infecting serotype, was initially higher than avidity to DENV3 in secondary DENV infections, the opposite relation was observed 3-18 months post-infection. We found significant correlations between IgM avidity and NT50 in acute primary cases and between IgG avidity and NT50 in secondary DENV infections. In summary, our findings indicate that IgM antibodies likely play a role in early control of DENV infections. IgG serum avidity to DENV, analyzed for the first time in longitudinal samples, switches from targeting mainly cross-reactive serotype(s to the current infecting serotype over time. Finally, serum avidity correlates with neutralization capacity.

  13. Retrospective analysis of prognostic factors in dengue infected patients with intracranial bleed

    Directory of Open Access Journals (Sweden)

    Sachidanand Gautam

    2016-01-01

    Conclusion: Very high index of suspicion is required in dengue infected patients for neurological complications, especially during the convalescence period. Special attention should be given to those patients who have altered sensorium, and should not be misinterpreted as fever delirium or toxic encephalopathy. It requires immediate attention and further neurological investigation (including thorough clinical examination. Timely diagnosis using a computed tomography scan and early neurosurgical intervention after rapid correction of thrombocytopenia can save many lives.

  14. Q Fever with Unusual Exposure History: A Classic Presentation of a Commonly Misdiagnosed Disease

    Directory of Open Access Journals (Sweden)

    Randall J. Nett

    2012-01-01

    Full Text Available We describe the case of a man presumptively diagnosed and treated for Rocky Mountain spotted fever following exposure to multiple ticks while riding horses. The laboratory testing of acute and convalescent serum specimens led to laboratory confirmation of acute Q fever as the etiology. This case represents a potential tickborne transmission of Coxiella burnetii and highlights the importance of considering Q fever as a possible diagnosis following tick exposures.

  15. Complement levels in Brazilian children during and after meningococcal meningitis Níveis de complemento em crianças brasileiras durante e após meningite meningocócica

    OpenAIRE

    Pérsio Roxo Júnior; Virgínia Paes Leme Ferriani; José Eduardo Teixeira; José Elpídio Barbosa

    2005-01-01

    PURPOSE: To evaluate the functional activity of the classical and alternative pathways of the complement system and the levels of C3, C4, and factor B during the first episode of meningococcal infection and during the convalescence period. PATIENTS AND METHODS: Ten Brazilian children ranging in age from 8 months to 8 years, admitted from 1991 to 1993 with a clinical-laboratory diagnosis of meningococcal meningitis, were studied during acute infection (up to 7 days from diagnosis) and during t...

  16. Readmissions after fast-track hip and knee arthroplasty

    DEFF Research Database (Denmark)

    Husted, Henrik; Otte, Niels Kristian Stahl; Kristensen, Billy B

    2010-01-01

    With the implementation of fast-track surgery with optimization of both logistical and clinical features, the postoperative convalescence has been reduced as functional milestones have been achieved earlier and consequently length of stay (LOS) in hospital has been reduced. However, it has been s...... speculated that a decrease in LOS may be associated with an increase in readmissions in general, including risk of dislocation after total hip arthroplasty (THA) or manipulation after total knee arthroplasty (TKA)....

  17. Dynamic changes of lipid acylhydroperoxide and Vitamin Eplasma levels in ischemic stroke patients

    Institute of Scientific and Technical Information of China (English)

    Huiming Mao; Ping Zhuang; Xianzhen Chen; Yongmin Yu; Bingguan Chen

    2008-01-01

    BACKGROUND: Studies have shown that malondialdehyde is the metabolic product of lipid peroxidation. However, detection of the intermediate metabolic product of lipid peroxidation, acylhydroperoxide (AHP), has rarely been reported. OBJECTIVE: To document concentrations of the intermediate metabolic product of lipid peroxidation, AHP, during different stages of ischemic cerebral infarction. DESIGN, TIME AND SETTING: Forty-eight patients with cerebral infarction from the East Hospital of Tongji University, and thirty-three students of Shanghai Geriatric University (healthy controls), in a case match-control study from April 2000 to January 2001. PARTICIPANTS: Based on different disease states, patients were separated into three groups: acute (n = 19), convalescent (n = 17), and chronic(n = 12). The healthy, control group consisted of 33 students, who were not treated with Vitamin E during the two weeks. METHODS: AHP concentration was detected by an ultraviolet spectrophotometric method, and plasma Vitamin E levels were detected by a visual spectrophotometric method. MAIN OUTCOME MEASURES: Plasma lipid AHP and Vitamin E levels of patients and healthy control groups. RESULTS: Plasma lipid AHP levels of patients in the acute group significantly increased compared with healthy control and convalescent groups (F = 4.40, P 0.05). No significant differences were detected in plasma lipid AHP and Vitamin E levels in the chronic group compared to the control and convalescent groups (P>0.05). CONCLUSIONS: Lipid pcroxidation, as evidenced by high plasma lipid AHP and low Vitamin E levels, occurs primarily during the acute stage of patients with cerebral infarction.

  18. 提高高性能战斗机飞行人员疗养效果的探讨

    Institute of Scientific and Technical Information of China (English)

    韩晓峰; 胡永杰; 王欣

    2013-01-01

      目的探讨提高高性能战斗机飞行员疗养效果的实施方法。方法分析生活管理、航空生理心理训练、自然疗养因子和心理辅导的作用。结果各种措施的实施可以改善高性能战斗机飞行人员的飞行疲劳、缓解心理紧张和促进健康恢复。结论通过整合利用上述措施,能够更好地提高高性能战斗机飞行人员的疗养效果。%  Objective To probe into the im plem entation m ethod to im prove the convalescent effect of flight personnel on high-per-form ance fighters.Methods Life m anagem ent,aviation physiological psychology training,natural convalescent factors and psychological counseling w ere analyzed.Results These m easures can help flight personnel on high-perform ance fighters relieve the flying fatigue,psy-chological stress and prom ote physical recovery.Conclusion The convalescent effect of flight personnel on high-perform ance fighters can be im proved by integrating the above m ethods.

  19. Gene expression profiles in peripheral blood mononuclear cells of SARS patients

    Institute of Scientific and Technical Information of China (English)

    Shi-Yan Yu; Yun-Wen Hu; Xiao-Ying Liu; Wei Xiong; Zhi-Tong Zhou; Zheng-Hong Yuan

    2005-01-01

    AIM: To investigate the role of inflammatory and anti-viral genes in the pathogenesis of SARS.METHODS: cDNA microarrays were used to screen the gene expression profiles of peripheral blood mononuclear cells (PBMCs) in two SARS patients (one in the acute severe phase and the other in the convalescent phase)and a healthy donor. In addition, real-time qualitative PCR was also performed to verify the reproducibility of the microarray results. The data were further analyzed.RESULTS: Many inflammatory and anti-viral genes were differentially expressed in SARS patients. Compared to the healthy control or the convalescent case, plenty of pro-inflammatory cytokines such as IL-1, TNF-α, IL-8, and MAPK signaling pathway were significantly upregulated in the acute severe case. However, anti-inflammatory agents such as IL-4 receptor, IL-13 receptor, IL-1Ra,and TNF-α-induced proteins 3 and 6 also increased dramatically in the acute severe case. On the contrary, a lot of IFN-stimulated genes like PKR, GBP-1 and 2, CXCL-10and 11, and JAK/STAT signal pathway were downregulated in the acute severe case compared to the convalescent case.CONCLUSION: Gene expression in SARS patients mirrors a host state of inflammation and anti-viral immunity at the transcription level, and understanding of gene expression profiles may make contribution to further studies of the SARS pathogenesis.

  20. Echographic findings in the late stages of Vogt-Koyanagi-Harada disease in mexican population

    Directory of Open Access Journals (Sweden)

    Mariana Mayorquín-Ruiz

    2014-12-01

    Full Text Available Purpose: To correlate clinical findings of Vogt-Koyanagi-Harada disease with standardized echography findings in a cross-sectional, descriptive and observational study. Methods: Patients with Vogt-Koyanagi-Harada disease in the convalescent and recurrence phases were evaluated with standardized ocular echography. Eyes with opaque media were excluded. Clinical findings were correlated with echographic data. Results: Thirty-seven eyes of 25 patients were included. Best corrected visual acuity was in average 20/100 (0.70 logMAR. Clinical findings included: sunset glow fundus (92%, pigment migration (92%, nummular chorioretinal depigmented scars (68% and subretinal fibrosis (64.8%. Standardized echography was able to recognize all the cases with subretinal fibrosis (n=24 described clinically. Standardized echography showed a 100% sensitivity and specificity of finding subretinal fibrosis. Subretinal fibrosis in patients with Vogt-Koyanagi-Harada represents a risk factor for low vision. In our patients’ eyes, presence of subretinal fibrosis had a 2.5 time relative risk of having a visual acuity equal or worst to 20/70. Conclusions: Standardized echography represents a useful tool in patients with VKH in the chronic (convalescence and recurrence phase of the disease. Subretinal fibrosis, a sight threatening complication in the convalescence and recurrent phases of Vogt-Koyanagi-Harada, can be diagnosed with ocular echography, with characteristic images. Knowledge of these images can be useful in cases with opaque media and bilateral anterior segment granulomatous inflammatory disease.

  1. 军队疗养院特勤人员疗养的实践与思考%Recuperation of Special Service Personnel in Military Sanatorium: Practice and Reflection

    Institute of Scientific and Technical Information of China (English)

    张晖

    2011-01-01

    Starting with the recuperation requirements of special service personnel, the article introduced the recuperate necessity for special service personnel with analysis on the recuperation situation. In order to over-come exhaustion, restore physical strength, enhance health, solidify and boost combat effectiveness, the author proposed that special service personnel must participate in planned convalescence. The sanatorium should strength-en the construction and research of the special service convalescences, explore new management pattern for the spe-cial service personnel, and put forward theoretical foundations for the construction and the reform for the convales-cence of special service personnel.%本文从特勤人员疗养需要入手,分析特勤人员疗养面临的形势和现状,提出特勤人员疗养的必要性.认为特勤人员必须有计划地安排健康疗养,达到消除疲劳、恢复体能,维护健康,巩固和提高部队战斗力的目的.疗养院应加强特勤疗养的建设和研究,积极探索特勤疗养管理的新模式,为特勤疗养建设和改革提供理论支持.

  2. Dengue Virus (DENV) Neutralizing Antibody Kinetics in Children After Symptomatic Primary and Postprimary DENV Infection.

    Science.gov (United States)

    Clapham, Hannah E; Rodriguez-Barraquer, Isabel; Azman, Andrew S; Althouse, Benjamin M; Salje, Henrik; Gibbons, Robert V; Rothman, Alan L; Jarman, Richard G; Nisalak, Ananda; Thaisomboonsuk, Butsaya; Kalayanarooj, Siripen; Nimmannitya, Suchitra; Vaughn, David W; Green, Sharone; Yoon, In-Kyu; Cummings, Derek A T

    2016-05-01

    The immune response to dengue virus (DENV) infection is complex and not fully understood. Using longitudinal data from 181 children with dengue in Thailand who were followed for up to 3 years, we describe neutralizing antibody kinetics following symptomatic DENV infection. We observed that antibody titers varied by serotype, homotypic vs heterotypic responses, and primary versus postprimary infections. The rates of change in antibody titers over time varied between primary and postprimary responses. For primary infections, titers increased from convalescence to 6 months. By comparing homotypic and heterotypic antibody titers, we saw an increase in type specificity from convalescence to 6 months for primary DENV3 infections but not primary DENV1 infections. In postprimary cases, there was a decrease in titers from convalescence up until 6 months after infection. Beginning 1 year after both primary and postprimary infections, there was evidence of increasing antibody titers, with greater increases in children with lower titers, suggesting that antibody titers were boosted due to infection and that higher levels of neutralizing antibody may be more likely to confer a sterilizing immune response. These findings may help to model virus transmission dynamics and provide baseline data to support the development of vaccines and therapeutics.

  3. Serological Evidence of Chikungunya Virus among Acute Febrile Patients in Southern Mozambique.

    Science.gov (United States)

    Gudo, Eduardo Samo; Pinto, Gabriela; Vene, Sirkka; Mandlaze, Arcildo; Muianga, Argentina Felisbela; Cliff, Julie; Falk, Kerstin

    2015-01-01

    In the last two decades, chikungunya virus (CHIKV) has rapidly expanded to several geographical areas, causing frequent outbreaks in sub-Saharan Africa, South East Asia, South America, and Europe. Therefore, the disease remains heavily neglected in Mozambique, and no recent study has been conducted. Between January and September 2013, acute febrile patients with no other evident cause of fever and attending a health center in a suburban area of Maputo city, Mozambique, were consecutively invited to participate. Paired acute and convalescent serum samples were requested from each participant. Convalescent samples were initially screened for anti-CHIKV IgG using a commercial indirect immunofluorescence test, and if positive, the corresponding acute sample was screened using the same test. Four hundred patients were enrolled. The median age of study participants was 26 years (IQR: 21-33 years) and 57.5% (224/391) were female. Paired blood samples were obtained from 209 patients, of which 26.4% (55/208) were presented anti-CHIKV IgG antibodies in the convalescent sample. Seroconversion or a four-fold titer rise was confirmed in 9 (4.3%) patients. The results of this study strongly suggest that CHIKV is circulating in southern Mozambique. We recommend that CHIKV should be considered in the differential diagnosis of acute febrile illness in Mozambique and that systematic surveillance for CHIKV should be implemented.

  4. Cellular and humoral immune responses to Borrelia burgdorferi antigens in patients with culture-positive early Lyme disease.

    Science.gov (United States)

    Vaz, A; Glickstein, L; Field, J A; McHugh, G; Sikand, V K; Damle, N; Steere, A C

    2001-12-01

    We determined cellular and humoral immune responses to Borrelia burgdorferi lysate and to recombinant flagellin (FlaB), OspC, and OspA in acute- and convalescent-phase samples from 39 culture-positive patients with erythema migrans and in 20 healthy control subjects. During the acute illness, a median of 4 days after the onset of erythema migrans, 51% of the patients had proliferative cellular responses and 72% had antibody responses to at least one of the borrelial antigens tested. During convalescence, at the conclusion of antibiotic therapy, 64% of the patients had proliferative cellular reactivity and 95% had antibody reactivity with at least one of the spirochetal antigens tested. In both acute- and convalescent-phase samples, cellular immune responses were found as frequently to OspA as to OspC and FlaB. Although antibody responses were also frequently seen to OspC and FlaB, only a few patients had marginal antibody reactivity with OspA. The percentage of patients with proliferative responses was similar in those with clinical evidence of localized or disseminated infection, whereas humoral reactivity was found more often in those with disseminated disease. We conclude that cellular and humoral responses to B. burgdorferi antigens are often found among patients with early Lyme disease. In contrast with the other antigens tested, cellular but not humoral reactivity was often found with OspA.

  5. Measles Virus-Specific Immunoglobulin G Isotype Immune Response in Early and Late Infections

    Science.gov (United States)

    Isa, María Beatríz; Martínez, Laura; Giordano, Miguel; Zapata, Marta; Passeggi, Carlos; De Wolff, María Cristina; Nates, Silvia

    2001-01-01

    A total of 154 human serum samples (32 acute-phase and 22 convalescent-phase serum samples obtained within a week and between days 8 and 26 after the onset of rash, respectively, and 100 samples drawn from healthy immune adults) were processed by an immunofluorescence assay for the detection of immunoglobulin M (IgM), total immunoglobulin G (IgG), IgG1, IgG2, IgG3, and IgG4 measles virus-specific antibodies. In the acute phase, IgG1 was seen first, followed by IgG2, IgG3, and IgG4 responses, the mean seropositivity of which gradually increased during convalescence, reaching 100% (standard deviation [SD], 84 to 100%), 57% (SD, 34 to 80%), 86% (SD, 66 to 100%), and 86% (SD, 66 to 100%), respectively. IgG2 rose and fell in connection with IgG3 subclass antibodies, showing a rate of detection of IgG2 and/or IgG3 subclass antibodies of 95.5% (range, 100 to 86.5%) in the convalescent phase of infection. The mean percentage of measles IgG2 and IgG3 seropositivity dropped significantly during the memory phase, to 2% (range, 2 to 6%) and 3% (range, 3 to 7%), respectively (P < 0.05); meanwhile IgG1 and IgG4 subclass responses remained relatively unmodified in samples obtained years after infection (mean 100% [SD, 96 to 100%] and 86% [SD, 79 to 93%], respectively). Results obtained defined two highly different immune isotypic response patterns. One pattern is restrictive to IgG2 and/or IgG3 in the convalescent phase and is kinetically similar to the IgM antibody response, so its detection could be referred to as a recent viral activity. On the other hand, IgG1 and IgG4 were detected in both the convalescent and memory phases of the immune response, but their isolated occurrence without IgG2 and IgG3 could be related to the long-lasting immunity. PMID:11136766

  6. 老年冠心病疗养员血脂相关性心血管剩留风险调查分析

    Institute of Scientific and Technical Information of China (English)

    范茂丹; 周磊; 杨敏

    2015-01-01

    Objective To study the lipid-low ering drug therapy status and the level of blood lipid param eters of aged convalescents with coronary heart disease and estim ate the blood lipid related residual cardiovascular risks of el-derly convalescents.Methods 362 aged convalescents with coronary heart disease w ere selected to collect lipid-low er-ing drug therapy status and m easure total cholesterol (TC ),triglyceride (TG ),low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol(HDL-C )and other lipid param eters,then to calculate the incidence of dys-lipidem ia.Results In aged convalescents with coronary heart disease,345 cases took statins alone,0case took fibrates alone,and 17 cases took statins and fibrates.The prevalence rates of hypercholesterolem ia,hypertriglyceridem ia,high LD L-C cholesterol,low HDL-C cholesterolw ere 9.94% (36/362),18.23% (66/362),5.80% (21/362),and 16.57% (60/362). Conclusion There are varying degrees of dyslipidem ia in aged convalescents with coronary heart disease.We should intensify lipid-low ering therapy and strengthen residual cardiovascular risk assessm ent and intervention,correct dyslipi-dem ia as early and com pletely as possible,so as to m inim ize the residual cardiovascular risks in aged convalescents with coronary heart disease.%目的:了解老年冠心病疗养员降脂药物治疗现状及血脂参数水平,评估该人群血脂相关性心血管剩留风险。方法共入选老年冠心病疗养员362例,收集降脂药物治疗情况并测量血清总胆固醇( TC )、三酰甘油( TG )、低密度脂蛋白胆固醇( LDL-C )、高密度脂蛋白胆固醇( HDL-C )等血脂参数,计算脂代谢异常发生率。结果老年冠心病疗养员中,单独服用他汀类药物者345例,单独服用贝特类药物者0例,联合服用他汀类及贝特类药物者17例。高胆固醇血症、高三酰甘油血症、高低密度脂蛋白胆固醇血症、低高密度脂蛋白胆固

  7. Establishment of animal model for potency evaluation of inactivated SARS virus experimental vaccine

    Institute of Scientific and Technical Information of China (English)

    GUANMUDONG; QIAN; YANKONG; WENXUELIU; LIHINGYANG; JUNZHIWANG; YONGXINYU; YAOLONGSHU; ZHENGWANG; WEIDONGYIN; QINGYUZHU; HAIFAZHENG

    2005-01-01

    The purpose of this study was to test the effectiveness of source virus strain for the manufacture of the inactivated SARS virus vaccine, and establish an experimental method and preliminary standard for potency evaluation. Mice were divided into groups for being immunized with corresponding serially diluted experimental SARS virus inactivated vaccine. And the rabbits were immunized with undiluted vaccine. Challenge assay was conducted with a heterologous SARS virus. And the neutralization antibody was determined with plaque reduction neutralization test(PRNT), to which the neutralization antibody in the convalescent serum of SARS patients was compared. The experimental vaccine viral strains were proved to be suitable for manufacturing the vaccine. Mice immunized by vaccines of serial dilutions were able to elicit neutralizing antibody. The antibody titer from mice immunized with the undiluted vaccine could reach up to 1:495.2, while those of rabbits immunized with the undiluted vaccine could reach a GMT of 55.0-79.9. The capability of the antibody to neutralize the virus from Guangdong is more efficient than that from Beijing. The GMT of neutralizing antibody in SARS convalescents living in south and north China ranged from 50.12 to 54.95, and the titers of convalescents from north China were higher than those from south China. Mice and rabbits used as the model for evaluation of potency are of sensitivity, and the test is of reproducibility. The candidate challenge viral strains showed a relatively consistent effect on evaluating antibodies produced by various batches and different vaccine-source strains,hence they can be used to evaluate potency of the vaccine. The method for testing the vaccine potency and the evaluation standard was established preliminarily.

  8. Viability and Functionality of Cryopreserved Peripheral Blood Mononuclear Cells in Pediatric Dengue.

    Science.gov (United States)

    Perdomo-Celis, Federico; Salgado, Doris M; Castañeda, Diana M; Narváez, Carlos F

    2016-05-01

    Cryopreserved peripheral blood mononuclear cells (PBMCs) are widely used in studies of dengue. In this disease, elevated frequency of apoptotic PBMCs has been described, and molecules such as soluble tumor necrosis factor (TNF)-related apoptosis-inducing ligands (sTRAIL) are involved. This effect of dengue may affect the efficiency of PBMC cryopreservation. Here, we evaluate the viability (trypan blue dye exclusion and amine-reactive dye staining) and functionality (frequency of gamma interferon [IFN-γ]-producing T cells after polyclonal stimulation) of fresh and cryopreserved PBMCs from children with dengue (in acute and convalescence phases), children with other febrile illnesses, and healthy children as controls. Plasma sTRAIL levels were also evaluated. The frequencies of nonviable PBMCs detected by the two viability assays were positively correlated (r = 0.74; P dengue, who had a higher frequency of nonviable cells than healthy children and children with other febrile illnesses (P ≤ 0.02), and PBMC viability levels were restored in the convalescent phase. In the acute phase, an increased frequency of CD3(+) CD8(+) amine-positive cells was found before cryopreservation (P = 0.01). Except for B cells in the acute phase, cryopreservation usually did not affect the relative frequencies of viable PBMC subpopulations. Dengue infection reduced the frequency of IFN-γ-producing CD3(+) cells after stimulation compared with healthy controls and convalescent-phase patients (P ≤ 0.003), and plasma sTRAIL correlated with this decreased frequency in dengue (rho = -0.56; P = 0.01). Natural dengue infection in children can affect the viability and functionality of cryopreserved PBMCs.

  9. High rate of subclinical chikungunya virus infection and association of neutralizing antibody with protection in a prospective cohort in the Philippines.

    Directory of Open Access Journals (Sweden)

    In-Kyu Yoon

    2015-05-01

    Full Text Available Chikungunya virus (CHIKV is a globally re-emerging arbovirus for which previous studies have indicated the majority of infections result in symptomatic febrile illness. We sought to characterize the proportion of subclinical and symptomatic CHIKV infections in a prospective cohort study in a country with known CHIKV circulation.A prospective longitudinal cohort of subjects ≥6 months old underwent community-based active surveillance for acute febrile illness in Cebu City, Philippines from 2012-13. Subjects with fever history were clinically evaluated at acute, 2, 5, and 8 day visits, and at a 3-week convalescent visit. Blood was collected at the acute and 3-week convalescent visits. Symptomatic CHIKV infections were identified by positive CHIKV PCR in acute blood samples and/or CHIKV IgM/IgG ELISA seroconversion in paired acute/convalescent samples. Enrollment and 12-month blood samples underwent plaque reduction neutralization test (PRNT using CHIKV attenuated strain 181/clone25. Subclinical CHIKV infections were identified by ≥8-fold rise from a baseline enrollment PRNT titer 50 years old. Baseline CHIKV PRNT titer ≥10 was associated with 100% (95%CI: 46.1, 100.0 protection from symptomatic CHIKV infection. Phylogenetic analysis demonstrated Asian genotype closely related to strains from Asia and the Caribbean.Subclinical infections accounted for a majority of total CHIKV infections. A positive baseline CHIKV PRNT titer was associated with protection from symptomatic CHIKV infection. These findings have implications for assessing disease burden, understanding virus transmission, and supporting vaccine development.

  10. Importance of nutritional status in recovery from acute cholecystitis: benefit from enteral nutrition supplementation including medium chain triglycerides.

    Science.gov (United States)

    Nomura, Yukinobu; Inui, Kazuo; Yoshino, Junji; Wakabayashi, Takao; Okushima, Kazumu; Kobayashi, Takashi; Miyoshi, Hironao; Nakamura, Yuta

    2007-09-01

    This study was undertaken to clarify the importance of nutritional status in patients with acute cholecystitis, and also evaluate whether they benefited from enteral nutrition supplementation, including medium-chain triglycerides (MCT), during the convalescent stage. Patients with acute cholecystitis admitted to our hospital between April 1994 and March 2002 were classified into a poor nutrition group (n=40; total serum proteinnutrition group (n=71; >5.0 g/dl). Patients with poor nutrition were significantly more elderly than those with fair nutrition, and had significantly higher serum C-reactive protein (CRP) concentrations. The two groups did not differ significantly with respect to other laboratory data, gender distribution, or medical treatment. We supplemented ordinary meals with enteral nutrition including MCT in 16 patients during the convalescent stage (MCT group). We compared their length of hospital stay and days required to recovery to pre-admission functional status for activities of daily living (ADL) with the same intervals in 16 patients without supplementation (non-MCT group) selected to match for age, gender, and fair or poor nutritional status from among 111 patients. Hospitalizations were significantly longer in the poor nutrition group (43.0+/-2.2 days) than in the fair nutrition group (27.0+/-8.2 days). Significantly more days were required to recover ADL status in the poor nutrition group (12.0+/-7.2 days) than in the fair group (9.4+/-5.2 days). Hospitalizations were significantly shorter in the MCT group (20.1+/-15 days) than in the non-MCT group (35.4+/-12.8 days). Significantly fewer days were required to recover ADL status in the MCT group (10.9+/-7 days) than in the non-MCT group (13.1+/-6.8 days). Administration of enteral nutrition including MCT during convalescence from acute cholecystitis thus appears to promote functional recovery shorten hospital stay.

  11. Evaluation of the diagnostic value of serologic microagglutination testing and a polymerase chain reaction assay for diagnosis of acute leptospirosis in dogs in a referral center.

    Science.gov (United States)

    Fraune, Claudia Kümmerle; Schweighauser, Ariane; Francey, Thierry

    2013-05-15

    To determine the diagnostic value of a serologic microagglutination test (MAT) and a PCR assay on urine and blood for the diagnosis of leptospirosis in dogs with acute kidney injury (AKI). Cross-sectional study. Animals-76 dogs with AKI in a referral hospital (2008 to 2009). Dogs' leptospirosis status was defined with a paired serologic MAT against a panel of 11 Leptospira serovars as leptospirosis-associated (n = 30) or nonleptospirosis-associated AKI (12). In 34 dogs, convalescent serologic testing was not possible, and leptospirosis status was classified as undetermined. The diagnostic value of the MAT single acute or convalescent blood sample was determined in dogs in which leptospirosis status could be classified. The diagnostic value of a commercially available genus-specific PCR assay was evaluated by use of 36 blood samples and 20 urine samples. Serologic acute testing of an acute blood sample had a specificity of 100% (95% CI, 76% to 100%), a sensitivity of 50% (33% to 67%), and an accuracy of 64% (49% to 77%). Serologic testing of a convalescent blood sample had a specificity of 92% (65% to 99%), a sensitivity of 100% (87% to 100%), and an accuracy of 98% (88% to 100%). Results of the Leptospira PCR assay were negative for all samples from dogs for which leptospirosis status could be classified. Serologic MAT results were highly accurate for diagnosis of leptospirosis in dogs, despite a low sensitivity for early diagnosis. In this referral setting of dogs pretreated with antimicrobials, testing of blood and urine samples with a commercially available genus-specific PCR assay did not improve early diagnosis.

  12. Identification of seroreactive proteins of Leptospira interrogans serovar copenhageni using a high-density protein microarray approach.

    Science.gov (United States)

    Lessa-Aquino, Carolina; Borges Rodrigues, Camila; Pablo, Jozelyn; Sasaki, Rie; Jasinskas, Algis; Liang, Li; Wunder, Elsio A; Ribeiro, Guilherme S; Vigil, Adam; Galler, Ricardo; Molina, Douglas; Liang, Xiaowu; Reis, Mitermayer G; Ko, Albert I; Medeiros, Marco Alberto; Felgner, Philip L

    2013-01-01

    Leptospirosis is a widespread zoonotic disease worldwide. The lack of an adequate laboratory test is a major barrier for diagnosis, especially during the early stages of illness, when antibiotic therapy is most effective. Therefore, there is a critical need for an efficient diagnostic test for this life threatening disease. In order to identify new targets that could be used as diagnostic makers for leptopirosis, we constructed a protein microarray chip comprising 61% of Leptospira interrogans proteome and investigated the IgG response from 274 individuals, including 80 acute-phase, 80 convalescent-phase patients and 114 healthy control subjects from regions with endemic, high endemic, and no endemic transmission of leptospirosis. A nitrocellulose line blot assay was performed to validate the accuracy of the protein microarray results. We found 16 antigens that can discriminate between acute cases and healthy individuals from a region with high endemic transmission of leptospirosis, and 18 antigens that distinguish convalescent cases. Some of the antigens identified in this study, such as LipL32, the non-identical domains of the Lig proteins, GroEL, and Loa22 are already known to be recognized by sera from human patients, thus serving as proof-of-concept for the serodiagnostic antigen discovery approach. Several novel antigens were identified, including the hypothetical protein LIC10215 which showed good sensitivity and specificity rates for both acute- and convalescent-phase patients. Our study is the first large-scale evaluation of immunodominant antigens associated with naturally acquired leptospiral infection, and novel as well as known serodiagnostic leptospiral antigens that are recognized by antibodies in the sera of leptospirosis cases were identified. The novel antigens identified here may have potential use in both the development of new tests and the improvement of currently available assays for diagnosing this neglected tropical disease. Further

  13. Identification of seroreactive proteins of Leptospira interrogans serovar copenhageni using a high-density protein microarray approach.

    Directory of Open Access Journals (Sweden)

    Carolina Lessa-Aquino

    Full Text Available Leptospirosis is a widespread zoonotic disease worldwide. The lack of an adequate laboratory test is a major barrier for diagnosis, especially during the early stages of illness, when antibiotic therapy is most effective. Therefore, there is a critical need for an efficient diagnostic test for this life threatening disease.In order to identify new targets that could be used as diagnostic makers for leptopirosis, we constructed a protein microarray chip comprising 61% of Leptospira interrogans proteome and investigated the IgG response from 274 individuals, including 80 acute-phase, 80 convalescent-phase patients and 114 healthy control subjects from regions with endemic, high endemic, and no endemic transmission of leptospirosis. A nitrocellulose line blot assay was performed to validate the accuracy of the protein microarray results.We found 16 antigens that can discriminate between acute cases and healthy individuals from a region with high endemic transmission of leptospirosis, and 18 antigens that distinguish convalescent cases. Some of the antigens identified in this study, such as LipL32, the non-identical domains of the Lig proteins, GroEL, and Loa22 are already known to be recognized by sera from human patients, thus serving as proof-of-concept for the serodiagnostic antigen discovery approach. Several novel antigens were identified, including the hypothetical protein LIC10215 which showed good sensitivity and specificity rates for both acute- and convalescent-phase patients.Our study is the first large-scale evaluation of immunodominant antigens associated with naturally acquired leptospiral infection, and novel as well as known serodiagnostic leptospiral antigens that are recognized by antibodies in the sera of leptospirosis cases were identified. The novel antigens identified here may have potential use in both the development of new tests and the improvement of currently available assays for diagnosing this neglected tropical disease

  14. Effect of physical restraint reduction on older patients' hospital length of stay.

    Science.gov (United States)

    Kwok, Timothy; Bai, Xue; Chui, Maria Y P; Lai, Claudia K Y; Ho, Daniel W H; Ho, Florence K Y; Woo, Jean

    2012-09-01

    Physical restraints are often used to prevent falls and to secure medical devices in older people in hospitals. Restraint reduction has been advocated on the grounds that physical restraints have negative psychological effects and are not effective in preventing falls. The potential effect of restraint reduction on length of hospital stay (LOS) has not been investigated. This study was undertaken to compare the average length of stay of older patients in a convalescent medical ward setting before and after a restraint reduction program. This is a retrospective study. A convalescent hospital in Hong Kong. This study included 2000 patient episodes. The use of physical restraint, LOS, and clinical outcomes of randomly selected patient episodes in the year before and after the implementation of a restraint reduction program were compared. The clinical outcomes included Modified Functional Ambulatory Categories and modified Barthel index. Subgroup analysis was performed on those with confusion as defined by dementia diagnosis, low abbreviated mental test score, or abnormal mental domain of Norton Score. A total of 958 and 988 patient episodes admitted to 10 medical wards in a convalescent hospital in 2007 and 2009 were examined. There were no significant differences in the baseline characteristics of patients in the 2 years. With the implementation of the restraint reduction scheme, the rate of physical restraint use declined significantly from 13.3% in 2007 to 4.1% in 2009 for all patients. The average LOS of patients was significantly lower in the year after the implementation of restraint reduction (19.5 ± 20.7 versus 16.8 ± 13.4 days in 2007 and 2009 respectively, P < .001). On subgroup analysis, the reduction in LOS was significant in the cognitively impaired patients (23.0 ± 26.5 to 17.8 ± 15.0 days in 2007 and 2009 respectively, P < .001), but not in the cognitively normal patients. There were no significant differences between the 2 years in the incidence of

  15. Interpretation of microscopic agglutination test for leptospirosis diagnosis and seroprevalence

    Institute of Scientific and Technical Information of China (English)

    Chintana Chirathaworn; Rajada Inwattana; Yong Poovorawan; Duangjai Suwancharoen

    2014-01-01

    Determination of antibody titer by microscopic agglutination test (MAT) has been used as a tool for leptospirosis diagnosis. Four fold or greater rise in antibody titers between acute and convalescent sera suggests recent Leptospira infection. In addition, results obtained by MAT have been used to predict infecting serovars. However, cross reactivity among various Leptospira serovars have been reported when patient sera were tested with a battery of Leptospira serovars. This study demonstrates cross- reactivity among several Leptospira serovars when MAT was performed on leptospirosis sera. The data support a role of MAT as a tool for diagnosis. However, for information on infecting serovars, Leptospira isolation and molecular identification should be performed.

  16. [Changes in mesenteric microcirculation in rats following repeated skin burns].

    Science.gov (United States)

    Shtykhno, Iu M

    1976-07-01

    Acute experiments were conducted on rats; repeated extensive burn of a convalescent who formerly sustained the burn disease was better tolerated, led tono fatal outcome and was accompanied by moderate microcirculatory disturbances. The smae burn was accompanied in intact rats by a severe shock followed by death, intravascular aggregation of erythrocytes and significant microcirculatory disturbances leading to disturbance of tissue nutrition. It is supposed that the results obtained could serve as an indirect proof that toxemia played an important role in the genesis of intravascular aggregation of erythrocytes in burn shock.

  17. Should close contacts of returning travellers with typhoid fever be protected by vaccination?

    Science.gov (United States)

    Kantele, A

    2015-03-17

    Increasing international travel to areas endemic for typhoid fever correlates with increased risk for travellers to contract the disease. At home, the acutely ill/convalescent patients may pose some risk to their close contacts. In Finland an unofficial guideline suggests vaccination for close contacts of patients with acute typhoid fever; in other developed countries, routine typhoid vaccinations are only recommended to contacts of chronic carriers. This paper discusses the possibilities and limitations of prophylactic/post-exposure typhoid vaccination for contacts of patients with acute disease. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Loss of cellular immune reactivity during acute Plasmodium falciparum malaria

    DEFF Research Database (Denmark)

    Hviid, L; Theander, T G; Abu-Zeid, Y A;

    1991-01-01

    Sixteen patients suffering from acute Plasmodium falciparum malaria were studied. All were residents of an area of unstable malaria-transmission in Eastern Sudan. Blood-samples were drawn at diagnosis, and 7 and 30 days later. Blood-samples from thirteen donors, drawn outside the malaria...... convalescence. Five donors examined by fluorescence-activated cell sorting (FACS) showed no increase in surface expression of IL-2 receptor on peripheral lymphocytes. The data indicate that acute P. falciparum malaria causes a depletion of antigen-reactive T-cells from the peripheral circulation, probably due...

  19. Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence properties

    Science.gov (United States)

    Orihuela, C. J.; Janssen, R.; Robb, C. W.; Watson, D. A.; Niesel, D. W.

    2000-01-01

    We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro.

  20. Pectus excavatum: a 15-year perspective.

    Science.gov (United States)

    Golladay, E S; Wagner, C W

    1991-09-01

    Pectus excavatum is relatively uncommon. Our experience with 177 children during a 15-year period produced changes in our surgical technique, which now includes a small transverse incision, minimal subcutaneous flap elevation, a muscle-relaxing incision over the fifth costal cartilage, complete resection of involved cartilage, use of Adkins' strut, suspension of sternum to strut, taut reefing of intercostal muscle, no tubes or drains, epidural analgesia, a patient-controlled analgesia device postoperatively, and eventual strut removal. Use of the evolved technique gives excellent cosmetic results, good functional results with minimal discomfort, and a shorter convalescent period.

  1. [Peritonitis caused by spontaneous rupture of pyonephrosis in pregnancy. Report of a case].

    Science.gov (United States)

    Rabii, R; Rais, H; Sarf, I; Joual, A; Aboutaieb, R; Bennani, S; el Mrini, M; Benjelloun, S; Hamoudi, D; Idali, B; Harti, A; Barrou, L

    1999-01-01

    Peritonitis after spontaneous rupture of pyonephrosis into the peritoneal cavity is a rare complication, usually diagnosed intraoperatively. We report a case of a woman presenting with left lumbar pain and fever during pregnancy. On admission, ultrasonography showed a pregnancy with fetal activity for 16 weeks, and pyonephrosis in the left kidney, but on a normal right kidney. After antibiotic therapy and upper urinary, tract stenting renal drainage revealed purulent urine, fever persisted with acute abdomen. Clinical and radiological assessment showed features of acute peritonitis with pyonephrosis. Treatment consisted of laparotomy with nephrectomy and abdominal lavage and drainage. The postoperative complication was septic shock requiring resuscitation and artificial ventilation and prolonged convalescence.

  2. Note from the CERN Medical Service FLU VACCINATION

    CERN Multimedia

    2000-01-01

    CERN Members of personnel who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 16th October and 30th November 2000. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  3. NOTE FROM THE CERN MEDICAL SERVICE - FLU VACCINATION

    CERN Multimedia

    2002-01-01

    People working on the CERN site who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  4. Note from the CERN Medical Service: FLU VACCINATION

    CERN Multimedia

    2003-01-01

    People working on the CERN site who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2003. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  5. Note from the CERN Medical Service FLU VACCINATION

    CERN Multimedia

    2001-01-01

    CERN Members of personnel who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 16th October and 30th November 2000. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  6. Note from the CERN Medical Service. FLU VACCINATION

    CERN Multimedia

    2001-01-01

    CERN Members of personnel who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 16th October and 30th November 2000. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  7. Note from the CERN Medical Service: FLU VACCINATION

    CERN Multimedia

    2001-01-01

    CERN Members of personnel who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 16th October and 30th November 2001. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  8. Note from the CERN Medical Service FLU VACCINATION

    CERN Multimedia

    Sylvain Weisz

    2002-01-01

    People working on the CERN site who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2002. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  9. Note from the CERN Medical Service FLU VACCINATION

    CERN Multimedia

    2003-01-01

    People working on the CERN site who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2003. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  10. Bilateral microvascular second toe transfer for bilateral post-traumatic thumb amputation.

    Science.gov (United States)

    Nehete, Rajendra; Nehete, Anita; Singla, Sandeep; Adhav, Harshad

    2012-01-01

    In bilateral thumb amputations, the functional impairment is serious and every attempt should be made to reconstruct the thumb. We report a case of bilateral post traumatic thumb amputation, reconstructed with bilateral second toe transfer. Only two such cases have been reported in literature so far. Though there are various modalities for the reconstruction of thumb, microvascular toe transfer has its own merits. The convalescent period is minimal with excellent function. It is bilaterally symmetric and aesthetically superior to the osteoplastic reconstruction. The technical details are discussed, and the long term functional and aesthetic results are presented.

  11. Bilateral microvascular second toe transfer for bilateral post-traumatic thumb amputation

    Directory of Open Access Journals (Sweden)

    Rajendra Nehete

    2012-01-01

    Full Text Available In bilateral thumb amputations, the functional impairment is serious and every attempt should be made to reconstruct the thumb. We report a case of bilateral post traumatic thumb amputation, reconstructed with bilateral second toe transfer. Only two such cases have been reported in literature so far. Though there are various modalities for the reconstruction of thumb, microvascular toe transfer has its own merits. The convalescent period is minimal with excellent function. It is bilaterally symmetric and aesthetically superior to the osteoplastic reconstruction. The technical details are discussed, and the long term functional and aesthetic results are presented.

  12. Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections.

    Science.gov (United States)

    Corman, V M; Müller, M A; Costabel, U; Timm, J; Binger, T; Meyer, B; Kreher, P; Lattwein, E; Eschbach-Bludau, M; Nitsche, A; Bleicker, T; Landt, O; Schweiger, B; Drexler, J F; Osterhaus, A D; Haagmans, B L; Dittmer, U; Bonin, F; Wolff, T; Drosten, C

    2012-12-06

    We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.

  13. Aphasia with elation, hypermusia, musicophilia and compulsive whistling.

    Science.gov (United States)

    Jacome, D E

    1984-03-01

    A musically naive patient with dominant fronto-temporal and anterior parietal infarct developed transcortical mixed aphasia. From early convalescence, he exhibited elated mood with hyperprosody and repetitive, spontaneous whistling and whistling in response to questions. He often spontaneously sang without error in pitch, melody, rhythm and lyrics, and spent long periods of time listening to music. His behaviour progressively improved in parallel with very good recovery of verbal skills. Musicality and singing are rarely tested at the bedside. Preservation of these abilities in aphasics might portend eventual recovery.

  14. Loss of cellular immune reactivity during acute Plasmodium falciparum malaria

    DEFF Research Database (Denmark)

    Hviid, L; Theander, T G; Abu-Zeid, Y A

    1991-01-01

    Sixteen patients suffering from acute Plasmodium falciparum malaria were studied. All were residents of an area of unstable malaria-transmission in Eastern Sudan. Blood-samples were drawn at diagnosis, and 7 and 30 days later. Blood-samples from thirteen donors, drawn outside the malaria...... convalescence. Five donors examined by fluorescence-activated cell sorting (FACS) showed no increase in surface expression of IL-2 receptor on peripheral lymphocytes. The data indicate that acute P. falciparum malaria causes a depletion of antigen-reactive T-cells from the peripheral circulation, probably due...

  15. Note from the CERN Medical Service FLU VACCINATION

    CERN Multimedia

    2000-01-01

    CERN Members of personnel who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 16th October and 30th November 2000. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  16. [Dysautonomia at 2 times in Guillain-Barré syndrome].

    Science.gov (United States)

    Guidon, C; Granthil, C; Djiane, P; François, G

    1986-01-01

    Autonomic dysfunction is described in a 63 year old woman suffering from Guillain-Barré syndrome. Bradyarrhythmias occurred during the convalescent phase, whereas neurologic and respiratory symptoms were improving; they were not related to bronchial aspiration. Their severity and the inefficiency of medical treatment led to the insertion of a demand pace-maker. Cardiac monitoring by the Holter method in severe cases of Guillain-Barré syndrome may detect potentially lethal arrhythmias, that may then be avoided by a demand pace-maker.

  17. West Nile Virus Infection in the Central Nervous System [version 1; referees: 3 approved

    Directory of Open Access Journals (Sweden)

    Evandro R. Winkelmann

    2016-01-01

    Full Text Available West Nile virus (WNV, a neurotropic single-stranded flavivirus has been the leading cause of arboviral encephalitis worldwide.  Up to 50% of WNV convalescent patients in the United States were reported to have long-term neurological sequelae.  Neither antiviral drugs nor vaccines are available for humans.  Animal models have been used to investigate WNV pathogenesis and host immune response in humans.  In this review, we will discuss recent findings from studies in animal models of WNV infection, and provide new insights on WNV pathogenesis and WNV-induced host immunity in the central nervous system.

  18. Latrodectism: variations in clinical manifestations provoked by Latrodectus species of spiders.

    Science.gov (United States)

    Maretić, Z

    1983-01-01

    The clinical picture caused by the bite of Latrodectus spp. is described, including the patient's general condition with agonizing pains, cramps, spasms, motor unrest, boardlike abdomen, "facies latrodectismica", sweating, oliguria, hypertension, anxiety, mental excitation and an extended convalescence. The notorious biters are mainly L. mactans, L. variolus, L. hesperus, L. tredecimguttatus, L. hasselti, L. indistinctus and L. menavodi, all of which appear to provoke somewhat similar clinical pictures. For some species, however, there is limited data and for the toxicity of some spiders, such as L. rhodesiensis, no data are available, However, it would appear that the clinical manifestations of the venom are similar in all species.

  19. Diagnosis of hantavirus infection in humans.

    Science.gov (United States)

    Mattar, Salim; Guzmán, Camilo; Figueiredo, Luis Tadeu

    2015-08-01

    Rodent-borne hantaviruses (family Bunyaviridae, genus Hantavirus) cause hantavirus pulmonary syndrome in the Americas and hemorrhagic fever with renal syndrome in Europe and Asia. The viruses are transmitted to humans mainly by inhalation of virus-contaminated aerosols of rodent excreta and secreta. Classic clinical hemorrhagic fever with renal syndrome occurs in five phases: fever, hypotension, oliguria, polyuria, and convalescence. Hantavirus pulmonary syndrome is a severe acute disease that is associated with respiratory failure, pulmonary edema and cardiogenic shock. The diagnosis of hantavirus infections in humans is based on clinical and epidemiological information as well as laboratory tests. We review diagnosis for hantavirus infections based on serology, PCR, immunochemistry and virus culture.

  20. Goya's deafness.

    Science.gov (United States)

    Smith, P E M; Chitty, C N; Williams, G; Stephens, D

    2008-12-01

    Francisco Goya (1746-1828), a major Spanish artist, became profoundly deaf aged 46 years, following an acute illness. Despite this, his success continued and he eventually died aged 82 years. His illness is sketchily documented in letters written during his convalescence, describing headache, deafness, tinnitus, unsteadiness and visual disturbance with recovery (apart from deafness) over three months. There was a milder similar illness two years before, suggesting a relapsing condition. Vogt-Koyanagi-Harada syndrome, although previously accepted as Goya's diagnosis, is not supported by the limited evidence. Susac's syndrome or Cogan's syndrome, although both rare, are more likely explanations.

  1. Rekonvalescens og sygemelding efter operation for lyskebrok

    DEFF Research Database (Denmark)

    Bay-Nielsen, Morten; Bisgaard, Thue

    2009-01-01

    leisure activities and development of complications. The literature provides no evidence to support an increased risk of recurrence with early resumption of physical activity after inguinal hernia repair. Patients should be encouraged to resume full activities immediately after surgery, only limited......The duration of convalescence after inguinal hernia repair is determined by a number of factors, among which are information and recommendations given by the surgeon and the general practitioner, level and duration of postoperative pain, type of repair, level of physical activity at work and during...

  2. Hemodynamic effects of acute digitalization several months after acute myocardial infarction.

    Science.gov (United States)

    Ressl, J; Jandová, R; Jebavý, P; Kasalický, J; Widimský, J

    1975-01-01

    Left ventricular function was investigated at rest and during exercise by heart catheterization in 15 patients 3-5 months after acute myocardial infarction. The effect of 1 mg digoxin i.v. in ten patients was correlated to placebo (saline solution) in five patients. A significant decrease of the left ventricular enddiastolic pressure, increase of left ventricular systolic ejection fraction and a shift of the left ventricular function curve to left upwards was found after digoxin with no changes in the placebo group. This beneficial effect of acute digitalization in patients convalescing from uncomplicated myocardial infarction without clinical signs of manifest heart failure could have therapeutic implication.

  3. Microbial Colonization in a New Intensive Care Burn Unit. A Prospective Cohort Study

    Science.gov (United States)

    1985-02-01

    after transfer to the convalescent ward were added to the Total 0 1 ICU patients and presented as a total. Analysis of fre- Enterobacter aerogenes ...ProvIdencia stuartil 0 4 ICU 4 4 Enterobacter agglomerons 3 0 Total 7 9• "•"".’-,••Pseudomonas putida 0 3 t’•••P*ICU indicates Intensive care unit...Staphylococcus aureus Klebsiela pneumonias 1 2 ICU 14 19 Total 18 20 Enterobacter cloacae 0 2 nt.c ussce- Streptococcus pneurnonlae 0 2"".Enterococcus peciesi

  4. 健康教育对疗养员改善口腔卫生状况的效果评价

    Institute of Scientific and Technical Information of China (English)

    石桦; 钟伟

    2012-01-01

      Objective To assess the effect of health education to oral hygiene improvement among convalescents.Methods Convales-cents at Qingdao No1.sanatorium of Jinan military region from July,2009 to June,2011 were randomly divided into experiment group and control group.Apart from conventional treatment,the experiment group were given extra oral health education.Before and after hospitaliza-tion,simplified oral hygiene index,dental plaque control rate,and bleeding on probing rate were detected.Results 106 convalescents were studied,57 of which were in the experiment group and 49 in the control group.After rehabilitation,the simplified oral hygiene index of the experiment group was clearly improved,and the dental plaque control rate and bleeding on probing rate also clearly lowered ( <0.05). Conclusion Health education can improve the oral hygienic condition of convalescents.%  目的评价口腔健康教育对疗养员的口腔卫生状况的改善效果.方法选择2009-07—2011-06在济南军区青岛第一疗养院疗养的疗养员为研究对象,将入选患者随机分为实验组和对照组,除常规疗养安排外,对实验组进行额外的口腔健康教育.在入院和出院时分别检测疗养员的简化口腔卫生指数、菌斑控制率和探诊出血率.结果共观察了106例疗养员,实验组57例,对照组49例.疗养结束时,与对照组相比,实验组人群的简化口腔卫生指数有明显改善,同时菌斑控制率和探诊出血率也显著降低(<0.05).结论口腔健康教育能改善疗养员的口腔卫生状况.

  5. Fast-track surgery-an update on physiological care principles to enhance recovery

    DEFF Research Database (Denmark)

    Kehlet, Henrik

    2011-01-01

    INTRODUCTION: The concept of fast-track surgery (enhanced recovery programs) has been evolved and been documented to be successful by decreasing length of stay, morbidity and convalescence across procedures. FUTURE STRATEGIES: However, there are several possibilities for further improvement of mo...... of the components of fast-track surgery, where surgical stress, fluid and pain management are key factors. There is an urgent need for better design of studies, especially in minimal invasive surgery to achieve maximal outcome effects when integrated into the fast-track methodology....

  6. Report of a joint DMRQC/Organon field trial to detect hepatitis A IgM by ELISA.

    Science.gov (United States)

    Supran, E M; Craske, J; Hart, R J; Kurtz, J B; Parry, J V; Skidmore, S J; Gardner, P S

    1983-10-01

    The results of a field trial of a joint DMRQC/Organon ELISA kit for the detection of hepatitis A IgM antibody are reported. The participating laboratories were asked to use the kit to test a panel of 360 specimens consisting of duplicate coded samples of 180 sera. The panel was also tested by MACRIA in the Virus Reference Laboratory, Colindale. The ELISA was shown to be specific and sensitive giving good discrimination between acute and late convalescent hepatitis A sera. It was proposed that the same cut-off control as is used in the RIA (equivalent to 10 RIA units) should be adopted for the ELISA also.

  7. Lack of Durable Cross-Neutralizing Antibodies Against Zika Virus from Dengue Virus Infection.

    Science.gov (United States)

    Collins, Matthew H; McGowan, Eileen; Jadi, Ramesh; Young, Ellen; Lopez, Cesar A; Baric, Ralph S; Lazear, Helen M; de Silva, Aravinda M

    2017-05-01

    Cross-reactive antibodies elicited by dengue virus (DENV) infection might affect Zika virus infection and confound serologic tests. Recent data demonstrate neutralization of Zika virus by monoclonal antibodies or human serum collected early after DENV infection. Whether this finding is true in late DENV convalescence (>6 months after infection) is unknown. We studied late convalescent serum samples from persons with prior DENV or Zika virus exposure. Despite extensive cross-reactivity in IgG binding, Zika virus neutralization was not observed among primary DENV infections. We observed low-frequency (23%) Zika virus cross-neutralization in repeat DENV infections. DENV-immune persons who had Zika virus as a secondary infection had distinct populations of antibodies that neutralized DENVs and Zika virus, as shown by DENV-reactive antibody depletion experiments. These data suggest that most DENV infections do not induce durable, high-level Zika virus cross-neutralizing antibodies. Zika virus-specific antibody populations develop after Zika virus infection irrespective of prior DENV immunity.

  8. Detection of Salivary IgA Antibodies Against the HlyE Antigen as a Diagnosis of Typhoid Fever.

    Science.gov (United States)

    Chin, Kai Ling; Redhuan, Nur Eliyana Mohd; Balaram, Prabha; Phua, Kia Kien; Ong, Eugene Boon Beng

    2016-06-01

    The Salmonella typhi (S. typhi) haemolysin E protein (HlyE) has been shown to be a sensitive and specific antigen for the detection of typhoid fever through the detection of anti-HlyE antibodies in sera. Saliva can also be a useful diagnostic fluid as it also contains antibodies against bacterial pathogens. This study aims to evaluate the potential detection of salivary anti-HlyE antibodies as a diagnosis of typhoid fever. Saliva was collected from acute typhoid patients (n=16) who presented at Hospital Universiti Sains Malaysia with prolonged fever of more than five days and were positive for S. Typhi blood culture. Saliva was also collected from convalescent typhoid patients (n=11), patients with other febrile fevers (n=15), and from healthy individuals (n=25). An ELISA was developed to detect the presence of IgA antibodies against HlyE in the saliva of typhoid patients. The acute typhoid group had a higher mean absorbance value of 1.496 compared to the convalescent typhoid (0.538), other febrile fevers (0.678), and healthy individuals (0.457) group. This study demonstrated the utility of salivary anti-HlyE IgA antibody as a biomarker for the diagnosis of typhoid fever. Follow-up studies with a larger sample size will allow the optimization of the sensitivity and specificity of the assay. This non-invasive method can be useful for mass screening programs.

  9. Arenavirus Glycan Shield Promotes Neutralizing Antibody Evasion and Protracted Infection.

    Directory of Open Access Journals (Sweden)

    Rami Sommerstein

    2015-11-01

    Full Text Available Arenaviruses such as Lassa virus (LASV can cause severe hemorrhagic fever in humans. As a major impediment to vaccine development, delayed and weak neutralizing antibody (nAb responses represent a unifying characteristic of both natural infection and all vaccine candidates tested to date. To investigate the mechanisms underlying arenavirus nAb evasion we engineered several arenavirus envelope-chimeric viruses and glycan-deficient variants thereof. We performed neutralization tests with sera from experimentally infected mice and from LASV-convalescent human patients. NAb response kinetics in mice correlated inversely with the N-linked glycan density in the arenavirus envelope protein's globular head. Additionally and most intriguingly, infection with fully glycosylated viruses elicited antibodies, which neutralized predominantly their glycan-deficient variants, both in mice and humans. Binding studies with monoclonal antibodies indicated that envelope glycans reduced nAb on-rate, occupancy and thereby counteracted virus neutralization. In infected mice, the envelope glycan shield promoted protracted viral infection by preventing its timely elimination by the ensuing antibody response. Thus, arenavirus envelope glycosylation impairs the protective efficacy rather than the induction of nAbs, and thereby prevents efficient antibody-mediated virus control. This immune evasion mechanism imposes limitations on antibody-based vaccination and convalescent serum therapy.

  10. Arenavirus Glycan Shield Promotes Neutralizing Antibody Evasion and Protracted Infection.

    Science.gov (United States)

    Sommerstein, Rami; Flatz, Lukas; Remy, Melissa M; Malinge, Pauline; Magistrelli, Giovanni; Fischer, Nicolas; Sahin, Mehmet; Bergthaler, Andreas; Igonet, Sebastien; Ter Meulen, Jan; Rigo, Dorothée; Meda, Paolo; Rabah, Nadia; Coutard, Bruno; Bowden, Thomas A; Lambert, Paul-Henri; Siegrist, Claire-Anne; Pinschewer, Daniel D

    2015-11-01

    Arenaviruses such as Lassa virus (LASV) can cause severe hemorrhagic fever in humans. As a major impediment to vaccine development, delayed and weak neutralizing antibody (nAb) responses represent a unifying characteristic of both natural infection and all vaccine candidates tested to date. To investigate the mechanisms underlying arenavirus nAb evasion we engineered several arenavirus envelope-chimeric viruses and glycan-deficient variants thereof. We performed neutralization tests with sera from experimentally infected mice and from LASV-convalescent human patients. NAb response kinetics in mice correlated inversely with the N-linked glycan density in the arenavirus envelope protein's globular head. Additionally and most intriguingly, infection with fully glycosylated viruses elicited antibodies, which neutralized predominantly their glycan-deficient variants, both in mice and humans. Binding studies with monoclonal antibodies indicated that envelope glycans reduced nAb on-rate, occupancy and thereby counteracted virus neutralization. In infected mice, the envelope glycan shield promoted protracted viral infection by preventing its timely elimination by the ensuing antibody response. Thus, arenavirus envelope glycosylation impairs the protective efficacy rather than the induction of nAbs, and thereby prevents efficient antibody-mediated virus control. This immune evasion mechanism imposes limitations on antibody-based vaccination and convalescent serum therapy.

  11. Acute parvovirus B19 infection in identical twins unmasking previously unidentified hereditary spherocytosis.

    Science.gov (United States)

    Forde, Donall G; Cope, Alison; Stone, Ben

    2014-07-29

    Identical Caucasian male twins, previously fit, presented 1 week apart with short histories of fever and lethargy. The twins were febrile at presentation with profound pancytopaenia and evidence of haemolysis. There was no rash or arthralgia. Both required multiple red cell transfusions. The twins had positive IgM serology for Epstein-Barr virus (EBV), cytomegalovirus (CMV) and parvovirus B19. EBV viral capsid antigen and Epstein-Barr nuclear antigen IgGs were also positive however, suggesting past EBV exposure. Parvovirus B19 DNA was detected from peripheral blood PCR; CMV and EBV DNA PCRs were negative. Convalescent serology demonstrated no evolution of the CMV serological response, that is no IgG to CMV developed which implies an initial non-specific polyclonal IgM response. The twins recovered fully over 7 days, the first with a course of prednisolone and the second spontaneously. They were diagnosed with hereditary spherocytosis on convalescent blood films. On further questioning, a family history of hereditary spherocytosis was eventually revealed. The twins' maternal grandmother was known to have the condition asymptomatically. Their mother had prior to this never been tested, but later bloods would reveal a compatible biochemical picture.

  12. Development of a quantitative PCR assay for monitoring Streptococcus agalactiae colonization and tissue tropism in experimentally infected tilapia.

    Science.gov (United States)

    Su, Y-L; Feng, J; Li, Y-W; Bai, J-S; Li, A-X

    2016-02-01

    Streptococcus agalactiae has become one of the most important emerging pathogens in the aquaculture industry and has resulted in large economic losses for tilapia farms in China. In this study, three pairs of specific primers were designed and tested for their specificities and sensitivities in quantitative real-time polymerase chain reactions (qPCRs) after optimization of the annealing temperature. The primer pair IGS-s/IGS-a, which targets the 16S-23S rRNA intergenic spacer region, was finally chosen, having a detection limit of 8.6 copies of S. agalactiae DNA in a 20 μL reaction mixture. Bacterial tissue tropism was demonstrated by qPCR in Oreochromis niloticus 5 days post-injection with a virulent S. agalactiae strain. Bacterial loads were detected at the highest level in brain, followed by moderately high levels in kidney, heart, spleen, intestines, and eye. Significantly lower bacterial loads were observed in muscle, gill and liver. In addition, significantly lower bacterial loads were observed in the brain of convalescent O. niloticus 14 days post-injection with several different S. agalactiae strains. The qPCR for the detection of S. agalactiae developed in this study provides a quantitative tool for investigating bacterial tissue tropism in infected fish, as well as for monitoring bacterial colonization in convalescent fish.

  13. Assessment of plaque assay methods for alphaviruses.

    Science.gov (United States)

    Juarez, Diana; Long, Kanya C; Aguilar, Patricia; Kochel, Tadeusz J; Halsey, Eric S

    2013-01-01

    Viruses from the Alphavirus genus are responsible for numerous arboviral diseases impacting human health throughout the world. Confirmation of acute alphavirus infection is based on viral isolation, identification of viral RNA, or a fourfold or greater increase in antibody titers between acute and convalescent samples. In convalescence, the specificity of antibodies to an alphavirus may be confirmed by plaque reduction neutralization test. To identify the best method for alphavirus and neutralizing antibody recognition, the standard solid method using a cell monolayer overlay with 0.4% agarose and the semisolid method using a cell suspension overlay with 0.6% carboxymethyl cellulose (CMC) overlay were evaluated. Mayaro virus, Una virus, Venezuelan equine encephalitis virus (VEEV), and Western equine encephalitis virus (WEEV) were selected to be tested by both methods. The results indicate that the solid method showed consistently greater sensitivity than the semisolid method. Also, a "semisolid-variant method" using a 0.6% CMC overlay on a cell monolayer was assayed for virus titration. This method provided the same sensitivity as the solid method for VEEV and also had greater sensitivity for WEEV titration. Modifications in plaque assay conditions affect significantly results and therefore evaluation of the performance of each new assay is needed.

  14. Clinical and pathogenesis interpretation of changes in the electrocardiogram in infectious diseases

    Directory of Open Access Journals (Sweden)

    Yu. P. Finogeev

    2010-01-01

    Full Text Available Analyzing the diagnostic value of clinical and electrocardiographic diagnosis of heart lesions in infectious diseases should be emphasized that obtained with the help of their information is a benchmark for assessing not only the functional state of the myocardium of these patients. Importantly, according to the clinical manifestations and electrocardiogram in infectious diseases can be detected miokardidistrofiyu, myocarditis, as well as all possible rhythm disturbances, myocardial infarction, etc. The basis of circulatory disorders is a common factor in central nervous system (brain as the most sensitive to the action of an infectious toxin. This, in turn, leads to dysfunction of the autonomic nervous system, since the latter is regulated by the cerebral cortex. Based on clinical experience revealed that approximately 15-20% of infectious patients during early convalescence, and especially of late convalescence there are various functional changes in the cardiovascular system, which at the height of the disease were not found. These changes include: painless myocardial ischemia, miokardidistrofiya, sick sinus syndrome, extrasystolic arrhythmia, early repolarization syndrome, a hyperfunction of the right atrium, incomplete block, right bundle branch block, etc.

  15. Use of human immunoglobulins as an anti-infective treatment: the experience so far and their possible re-emerging role.

    Science.gov (United States)

    Bozzo, Jordi; Jorquera, Juan I

    2017-06-01

    Pooled human immunoglobulins (IGs) are prepared from plasma obtained from healthy donors as a concentrated antibody-containing solution. In addition, high-titer IGs (hyperimmune) against a specific pathogen can be obtained from vaccinated or convalescing donors. Currently, IGs can be used for the treatment of a variety of infections for which no specific therapy exists or that remain difficult to treat. Moreover, the recent pathogen outbreaks for which there is no approved treatment have renewed attention to the role of convalescent plasma and IGs. Areas covered: In this review, a historical perspective of the use of sera and IGs in humans as anti-infective agents (any viral, bacterial, parasitic infection), excluding immunodeficient patients, is presented from early development to the latest clinical studies. A Medline search was conducted to examine the peer-reviewed literature, with no date limits. Expert commentary: Human pooled plasma-derived IG products benefit from the polyclonal response of every individual donor and from the interindividual variability in such response. The trend to increased availability of vaccines for infectious diseases also opens new potential applications of hyperimmune IGs for emerging or re-emerging infectious diseases (e.g.: Ebola, Zika, Dengue), for the prevention and treatment in the general population, healthcare personnel and caregivers.

  16. Induction of immunomodulatory miR-146a and miR-155 in small intestinal epithelium of Vibrio cholerae infected patients at acute stage of cholera

    Science.gov (United States)

    Melgar, Silvia; Aung, Kyaw Min; Rahman, Arman; Qadri, Firdausi; Wai, Sun Nyunt; Shirin, Tahmina

    2017-01-01

    The potential immunomodulatory role of microRNAs in small intestine of patients with acute watery diarrhea caused by Vibrio cholerae O1 or enterotoxigenic Escherichia coli (ETEC) infection was investigated. Duodenal biopsies were obtained from study-participants at the acute (day 2) and convalescent (day 21) stages of disease, and from healthy individuals. Levels of miR-146a, miR-155 and miR-375 and target gene (IRAK1, TRAF6, CARD10) and 11 cytokine mRNAs were determined by qRT-PCR. The cellular source of microRNAs in biopsies was analyzed by in situ hybridization. The ability of V. cholerae bacteria and their secreted products to cause changes in microRNA- and mRNA levels in polarized tight monolayers of intestinal epithelial cells was investigated. miR-146a and miR-155 were expressed at significantly elevated levels at acute stage of V. cholerae infection and declined to normal at convalescent stage (Pcholerae O1 strain C6706 and clinical isolates from two study-participants, caused significant increase in miR-155 and miR-146a by the strain C6706 (Pcholera. The inducer is probably the V. cholerae bacterium. By inducing microRNAs the bacterium can limit the innate immune response of the host, including inflammation evoked by its own secreted factors, thereby decreasing the risk of being eliminated. PMID:28319200

  17. Induction of immunomodulatory miR-146a and miR-155 in small intestinal epithelium of Vibrio cholerae infected patients at acute stage of cholera.

    Science.gov (United States)

    Bitar, Aziz; De, Rituparna; Melgar, Silvia; Aung, Kyaw Min; Rahman, Arman; Qadri, Firdausi; Wai, Sun Nyunt; Shirin, Tahmina; Hammarström, Marie-Louise

    2017-01-01

    The potential immunomodulatory role of microRNAs in small intestine of patients with acute watery diarrhea caused by Vibrio cholerae O1 or enterotoxigenic Escherichia coli (ETEC) infection was investigated. Duodenal biopsies were obtained from study-participants at the acute (day 2) and convalescent (day 21) stages of disease, and from healthy individuals. Levels of miR-146a, miR-155 and miR-375 and target gene (IRAK1, TRAF6, CARD10) and 11 cytokine mRNAs were determined by qRT-PCR. The cellular source of microRNAs in biopsies was analyzed by in situ hybridization. The ability of V. cholerae bacteria and their secreted products to cause changes in microRNA- and mRNA levels in polarized tight monolayers of intestinal epithelial cells was investigated. miR-146a and miR-155 were expressed at significantly elevated levels at acute stage of V. cholerae infection and declined to normal at convalescent stage (Pcholerae O1 strain C6706 and clinical isolates from two study-participants, caused significant increase in miR-155 and miR-146a by the strain C6706 (Pcholera. The inducer is probably the V. cholerae bacterium. By inducing microRNAs the bacterium can limit the innate immune response of the host, including inflammation evoked by its own secreted factors, thereby decreasing the risk of being eliminated.

  18. Parenteral nourishment of patients undergoing surgical or traumatic stress.

    Science.gov (United States)

    Gilder, H

    1986-01-01

    Severe surgical or other traumatic stress initiates an integrated central nervous system and metabolic response characterized by catabolism which selectively preserves vital organs, drawing on peripheral tissue proteins for required amino acids. When oral intake is prohibited adequate intravenous nutritional support hastens convalescence and may be life-saving. Intravenous nutrients routinely consist of amino acids for replacement of lost protein, a nonprotein calorie source--usually glucose, and vitamins and minerals. Lipid, infrequently used in routine surgery as part of the calorie source, supplies essential fatty acids and prevents side effects resulting with large amounts of intravenous glucose. Lipid has other benefits. Stress-induced hormones stimulate lipid catabolism. When lipid is used for part of the calorie requirement in intravenous feedings, the plasma insulin level is reduced and peripheral amino acids become available for synthesis of critically needed visceral proteins. Recent work has shown that the branched chain amino acids carnitine and some species of lipid added to intravenous nutrient formulations postoperatively affect the nitrogen retention and may hasten convalescence. Further work should be directed at understanding the unique biochemical changes occurring after injury, devising objective assay procedures to measure the severity of the response and improving intravenous formulations for the acutely ill surgical patient.

  19. Treatment and nursing progress on patients with stroke combined emotion disorder%脑卒中合并情感障碍病人的治疗及护理进展

    Institute of Scientific and Technical Information of China (English)

    程素芹; 杜乐英

    2010-01-01

    It simply describes the treatment of patients with stroke combined emotion disorder. It puts forward the key points of nursing care for patients with stroke combined emotion disorder, that is to observe the conditions, prevent complications of critical patients in acute stage, and to nurse with medicine. The stress on nursing care in convalescent period is strengthening effective social support, controlling complications effectively and learning psychological knowledge, which is the leading way to alleviate emotion disorder and improve the quality of life in patients with stroke during convalescence period.%综述脑卒中合并情感障碍的治疗要点,提出脑卒中合并情感障碍病人的急性期应注意病情观察、防止并发症的发生,用药护理.恢复期的护理重点是加强有效的社会支持、并发症的有效控制及相关心理健康知识的学习,以改善脑卒中病人的情感障碍,提高生活质量.

  20. Connotation of Green Sanatorium and the Construction%绿色疗养院的内涵建设

    Institute of Scientific and Technical Information of China (English)

    徐承金

    2012-01-01

    In the process of construct modern sanatorium, we put forward the new concept of green sanatorium, which is a service mode integrating natural convalescent factors, with the rehabilitation technology and humanistic care. With the green sanatorium, the convalescent patient can accept the disease treatment, functional recovery, relaxed, natural new feeling. Green sanatorium has developed the Chinese tradition harmony health concepts , adapted to the demands of biological - psychological - social mode of modern medicine, and the sustainable development strategy.%在创建现代化疗养院实践过程中,笔者提出绿色疗养新概念.绿色疗养是融合自然疗养因子、康复技术与人文关怀与一体的综合型疗养模式,通过采取综合性疗养措施,使疗养员达到疾病矫治,功能恢复,身心放松,回归自然的全新感觉.绿色疗养秉承传统中医天人合一的养生理念,结合生物-心理-社会的现代医学模式,符合可持续发展战略,使疗养员的身心健康达到一个新的水平,提高疗养效果.

  1. Children with invasive Staphylococcus aureus disease exhibit a potently neutralizing antibody response to the cytotoxin LukAB.

    Science.gov (United States)

    Thomsen, Isaac P; Dumont, Ashley L; James, David B A; Yoong, Pauline; Saville, Benjamin R; Soper, Nicole; Torres, Victor J; Creech, C Buddy

    2014-03-01

    Despite the importance of Staphylococcus aureus as a common invasive bacterial pathogen, the humoral response to infection remains inadequately defined, particularly in children. The purpose of this study was to assess the humoral response to extracellular staphylococcal virulence factors, including the bicomponent leukotoxins, which are critical for the cytotoxicity of S. aureus toward human neutrophils. Children with culture-proven S. aureus infection were prospectively enrolled and stratified by disease type. Fifty-three children were enrolled in the study, of which 90% had invasive disease. Serum samples were obtained during the acute (within 48 h) and convalescent (4 to 6 weeks postinfection) phases, at which point both IgG titers against S. aureus exotoxins were determined, and the functionality of the generated antibodies was evaluated. Molecular characterization of clinical isolates was also performed. We observed a marked rise in antibody titer from acute-phase to convalescent-phase sera for LukAB, the most recently described S. aureus bicomponent leukotoxin. LukAB production by the isolates was strongly correlated with cytotoxicity in vitro, and sera containing anti-LukAB antibodies potently neutralized cytotoxicity. Antibodies to S. aureus antigens were detectable in healthy pediatric controls but at much lower titers than in sera from infected subjects. The discovery of a high-titer, neutralizing antibody response to LukAB during invasive infections suggests that this toxin is produced in vivo and that it elicits a functional humoral response.

  2. Prophylaxis and therapy for Chikungunya virus infection.

    Science.gov (United States)

    Couderc, Thérèse; Khandoudi, Nassirah; Grandadam, Marc; Visse, Catherine; Gangneux, Nicolas; Bagot, Sébastien; Prost, Jean-François; Lecuit, Marc

    2009-08-15

    Chikungunya virus (CHIKV) is a recently reemerged arbovirus responsible for a massive outbreak of infection in the Indian Ocean region and India that has a very significant potential to spread globally because of the worldwide distribution of its mosquito vectors. CHIKV induces a usually self-limited disease in humans that is characterized by fever, arthralgia, myalgia, and rash; however, cases of severe CHIKV infection have recently been described, particularly in adults with underlying condition and neonates born to viremic mothers. Human polyvalent immunoglobulins were purified from plasma samples obtained from donors in the convalescent phase of CHIKV infection, and the preventive and curative effects of these immunoglobulins were investigated in 2 mouse models of CHIKV infection that we developed. CHIKV immunoglobulins contain anti-CHIKV antibodies and exhibit a high in vitro neutralizing activity and a powerful prophylactic and therapeutic efficacy against CHIKV infection in vivo, including in the neonate. Administration of CHIKV immunoglobulins may constitute a safe and efficacious prevention strategy and treatment for individuals exposed to CHIKV who are at risk of severe infection, such as neonates born to viremic mothers and adults with underlying conditions. These results provide a proof-of-concept for purifying human immunoglobulins from plasma samples from patients in the convalescent phase of an emerging infectious disease for which neither prevention nor treatment is available.

  3. Hipbone Biomechanical Finite Element Analysis and Clinical Study after the Resection of Ischiopubic Tumors

    Institute of Scientific and Technical Information of China (English)

    Ya-qi He; Xue-lin Zhang; Bing-hang Tang; Ang Yang

    2012-01-01

    Objective To investigate the changes of hipbone biomechanics after the resection of ischiopubic tumors and their relationships with the complications in the convalescent stage,and directing the postoperative pelvic reconstruction.Methods DICOM data were used to create an intact hipbone finite element model and postoperative model.The biomechanical indices on the same region in the two models under the same boundary condition were compared.The differences of displacement,stress,and strain of the two models were analyzed with statistical methods.Results The distribution areas of the hipbone nodes' displacement,stress,and strain were similar before and after the simulated operation.The sacroiliac joint nodes' displacement (P=0.040) and strain (P=0.000),and the acetabular roof nodes' stress (P=0.000) and strain (P=0.005) of two models had signifi-cant differences,respectively.But the sacroiliac joint nodes' stress (P=0.076) and the greater sciatic notch nodes' stress (P=0.825) and strain (P=0.506) did not have significant differences.Conclusions The resection of ischiopubic tumors mainly affect the biomechanical states of the homolateral sacroiliac joint and acetabular roof.The complications in the convalescent stage are due to the biomechanical changes of the sacroiliac joint and the acetabular roof and disappearances of the stabilization and connection functions of the pubic symphysis and superior ramus of pubis.

  4. Selection of antigenically advanced variants of seasonal influenza viruses

    Science.gov (United States)

    Ozawa, Makoto; Taft, Andrew S.; Das, Subash C.; Hanson, Anthony P.; Song, Jiasheng; Imai, Masaki; Wilker, Peter R.; Watanabe, Tokiko; Watanabe, Shinji; Ito, Mutsumi; Iwatsuki-Horimoto, Kiyoko; Russell, Colin A.; James, Sarah L.; Skepner, Eugene; Maher, Eileen A.; Neumann, Gabriele; Kelso, Anne; McCauley, John; Wang, Dayan; Shu, Yuelong; Odagiri, Takato; Tashiro, Masato; Xu, Xiyan; Wentworth, David E.; Katz, Jacqueline M.; Cox, Nancy J.; Smith, Derek J.; Kawaoka, Yoshihiro

    2016-01-01

    Influenza viruses mutate frequently, necessitating constant updates of vaccine viruses. To establish experimental approaches that may complement the current vaccine strain selection process, we selected antigenic variants from human H1N1 and H3N2 influenza virus libraries possessing random mutations in the globular head of the haemagglutinin protein (which includes the antigenic sites) by incubating them with human and/or ferret convalescent sera to human H1N1 and H3N2 viruses. Further, we selected antigenic escape variants from human viruses treated with convalescent sera and from mice that had been previously immunized against human influenza viruses. Our pilot studies with past influenza viruses identified escape mutants that were antigenically similar to variants that emerged in nature, establishing the feasibility of our approach. Our studies with contemporary human influenza viruses identified escape mutants before they caused an epidemic in 2014–2015. This approach may aid in the prediction of potential antigenic escape variants and the selection of future vaccine candidates before they become widespread in nature. PMID:27572841

  5. 中草药浸浴烧伤病人康复期微循环变化%Alteration of microcirculation in burn rehabilitation stage by herbal bathing

    Institute of Scientific and Technical Information of China (English)

    冯玉柱; 徐刚; 姜海; 纪文军; 刘君; 周建红

    2002-01-01

    Objective To observe the change of microcirculation of burn patient in convalescent stage through treatment with herbal bathing. Method 40 burnpatients were bathed by Chinese herbal medicine in convalescent stage. Before and after bathing, the microcirculation of foot nail fold was observed by WX 753B microcirculation microscope and system and system of figure management. And the results were compared with normal person. Result The foot nail fold microcirculation changes of burn rehabilitation were remarkable higher than the control group (P< 0.01) on the malformation rate of capillary loop, and at the same time the patients were accompanied with increase of hidro duct (P< 0.01) and effusion of capillary loop (P< 0.01). The malformation rate of capillary loop of the foot nail fold was positive improved, the effusion and dripping of capillary loop was significant decreased after treatment with bathing. Conclusion Though the wound surface is healed, the microcirculation disturbance of burn patients exists in rehabilitation stage. And herbal bathing could effectively improve microcirculation.

  6. Cross-reactivity of human monoclonal antibodies generated with peripheral blood lymphocytes from dengue patients with Japanese encephalitis virus

    Directory of Open Access Journals (Sweden)

    Pipattanaboon C

    2013-08-01

    Full Text Available Chonlatip Pipattanaboon,1,3,8,* Tadahiro Sasaki,2,8,* Mitsuhiro Nishimura,2,8 Chayanee Setthapramote,1,8 Pannamthip Pitaksajjakul,1,4,8 Pornsawan Leaungwutiwong,1,3,8 Kriengsak Limkittikul,5,8 Orapim Puiprom,6 Mikiko Sasayama,6 Panjaporn Chaichana,6 Tamaki Okabayashi,6 Takeshi Kurosu,2,8 Ken-ichiro Ono,7,8 Pongrama Ramasoota,1,4,8 Kazuyoshi Ikuta2,8 1Center of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; 2Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan; 3Department of Microbiology and Immunology, 4Department of Social and Environmental Medicine, 5Department of Tropical Pediatrics, Faculty of Tropical Medicine, Mahidol University, 6Mahidol-Osaka Center for Infectious Diseases, Bangkok, Thailand; 7Medical and Biological Laboratories Corporation Ltd, Nagano, Japan; 8JST/JICA, Science and Technology Research Partnership for Sustainable Development, Tokyo, Japan *These authors made an equal contribution to this study Background: Hybridomas that produce human monoclonal antibodies (HuMAbs against Dengue virus (DV had been prepared previously using peripheral blood lymphocytes from patients with DV during the acute and convalescent phases of a secondary infection. Anti-DV envelope glycoprotein (E 99 clones, anti-DV premembrane protein (prM 8 clones, and anti-DV nonstructural protein 1 (NS1 4 clones were derived from four acute-phase patients, and anti-DV E 2 clones, anti-DV prM 2 clones, and anti-DV NS1 8 clones were derived from five convalescent-phase patients. Methods and results: In the present study, we examined whether these clones cross-reacted with Japanese encephalitis virus (JEV, which belongs to the same virus family. Forty-six of the above-described 99 (46/99 anti-E, 0/8 anti-prM, and 2/4 anti-NS1 HuMAbs from acute-phase, and 0/2 anti-E, 0/2 anti-prM, and 5/8 anti-NS1 HuMAbs from convalescent-phase showed neutralizing activity against

  7. 体育疗法在涉核特勤疗养中的应用

    Institute of Scientific and Technical Information of China (English)

    闫明启; 颜晗

    2015-01-01

    在现代高技术战争背景及各种复杂环境条件下,涉核官兵所必需具有的坚强意志和强健体魄是顺利完成各项军事任务的保证。如何有效提升涉核特勤疗养人员的体能状况仍然有待进一步研究探讨。本文从涉核特勤疗养人员的特点、涉核特勤疗养的必要性、体育疗法的组织与实施以及存在问题和改进措施等方面进行阐述,为如何将体育疗法合理应用于涉核特勤疗养人员体能训练提供一些有益的内容和参考。%In the background of m odern high technology w ar and com plex environm ental conditions,nucleus-in-volved officers and m en m ust have a strong w ill and a robust physique,w hich can ensure the successful accom plish-m ent of various m ilitary tasks.It still needs further research and discussion about how to effectively im prove the physi-cal stam ina status of the nucleus-involved special service convalescents.T his paper carries on the elaboration from the follow ing aspects such as the characteristics of nucleus-involved special service convalescents,the necessity of special service recuperation,the organization and im plem entation of physical exercise therapy,and the existing problem s and im provem ent m easures,w hich provides som e useful inform ation and reference on how to m ake reasonable application of physical exercise therapy for the physical training of nucleus-involved special service convalescents.

  8. Typhoid fever in young children in Bangladesh: clinical findings, antibiotic susceptibility pattern and immune responses.

    Science.gov (United States)

    Khanam, Farhana; Sayeed, Md Abu; Choudhury, Feroza Kaneez; Sheikh, Alaullah; Ahmed, Dilruba; Goswami, Doli; Hossain, Md Lokman; Brooks, Abdullah; Calderwood, Stephen B; Charles, Richelle C; Cravioto, Alejandro; Ryan, Edward T; Qadri, Firdausi

    2015-04-01

    Children bear a large burden of typhoid fever caused by Salmonella enterica serotype Typhi (S. Typhi) in endemic areas. However, immune responses and clinical findings in children are not well defined. Here, we describe clinical and immunological characteristics of young children with S. Typhi bacteremia, and antimicrobial susceptibility patterns of isolated strains. As a marker of recent infection, we have previously characterized antibody-in-lymphocyte secretion (TPTest) during acute typhoid fever in adults. We similarly assessed membrane preparation (MP) IgA responses in young children at clinical presentation, and then 7-10 days and 21-28 days later. We also assessed plasma IgA, IgG and IgM responses and T cell proliferation responses to MP at these time points. We compared responses in young children (1-5 years) with those seen in older children (6-17 years), adults (18-59 years), and age-matched healthy controls. We found that, compared to age-matched controls patients in all age cohorts had significantly more MP-IgA responses in lymphocyte secretion at clinical presentation, and the values fell in all groups by late convalescence. Similarly, plasma IgA responses in patients were elevated at presentation compared to controls, with acute and convalescent IgA and IgG responses being highest in adults. T cell proliferative responses increased in all age cohorts by late convalescence. Clinical characteristics were similar in all age cohorts, although younger children were more likely to present with loss of appetite, less likely to complain of headache compared to older cohorts, and adults were more likely to have ingested antibiotics. Multi-drug resistant strains were present in approximately 15% of each age cohort, and 97% strains had resistance to nalidixic acid. This study demonstrates that S. Typhi bacteremia is associated with comparable clinical courses, immunologic responses in various age cohorts, including in young children, and that TPTest can be used

  9. Improving performance management for delivering appropriate care for patients no longer needing acute hospital care.

    Science.gov (United States)

    Penney, Christine; Henry, Effie

    2008-01-01

    The public, providers and policy-makers are interested in a service continuum where care is provided in the appropriate place. Alternate level of care is used to define patients who no longer need acute care but remain in an acute care bed. Our aims were to determine how subacute care and convalescent care should be defined in British Columbia (BC); how these care levels should be aligned with existing legislation to provide more consistent service standards to patients and what reporting requirements were needed for system planning and performance management. A literature review was conducted to understand the international trends in performance management, care delivery models and change management. A Canada-wide survey was carried out to determine the directions of other provinces on the defined issues and a BC survey provided a current state analysis of programming within the five regional health authorities (HAs). A provincial policy framework for subacute and convalescent care has been developed to begin to address the concerns raised and provide a base for performance measurement. The policy has been approved and disseminated to BC HAs for implementation. An implementation plan has been developed and implementation activities have been integrated into the work of existing provincial committees. Evaluation will occur through performance measurement. The benefits anticipated include: clear policy guidance for programme development; improved comparability of performance information for system monitoring, planning and integrity of the national acute care Discharge Abstracting Database; improved efficiency in acute care bed use; and improved equity of access, insurability and quality for patients requiring subacute and convalescent care. While a national reporting system exists for acute care in Canada, this project raises questions about the implications for this system, given the shifting definition of acute care as other care levels emerge. Questions are also

  10. 温馨交流卡在老年荣军疗养患者健康教育中的应用

    Institute of Scientific and Technical Information of China (English)

    陈青凤

    2012-01-01

      Objective To investigate the application effect of warm communication cards in health education on elderly disabled soldier convalescents.Methods 300 cases of hospitalized convalescents were randomly divided into the observation group and the control group with 150 cases in each group.The control group was given conventional health education according to the different stages of hospitalization. The observation group was given self-designed warm communication cards for health education besides the conventional health education. Results The differences of health education knowledge grasped by the two groups were of statistic significance( <0.05);96% of the ob-servation group was satisfied with the form and content of the communication card.Conclusion Warm communication cards can be easily accepted by elderly disabled soldier convalescents for health education,thus improve the effect of health education and nursing quality.%  目的探讨温馨交流卡在老年荣军疗养患者健康教育中的应用效果.方法将300例内科住院患者随机分为观察组与对照组各150例.对照组根据患者住院的不同阶段按以往的方法实施健康教育.观察组在此基础上采用自行设计的温馨交流卡进行健康教育.结果两组患者对健康教育知识的掌握程度比较差异有统计学意义(<0.05);观察组96%的患者对交流卡的形式和内容感到满意.结论温馨交流卡用于老年荣军疗养患者健康教育易被患者接受,提高了健康教育效果及护理质量.

  11. Treatment of Infantile Cerebral Palsy Mainly by Acupoint-pressing and Tuina

    Institute of Scientific and Technical Information of China (English)

    ZHENG Wei-guo

    2005-01-01

    目的:探讨点穴、推拿、穴位注射、运动训练综合康复治疗小儿脑瘫的方法和疗效.方法:将640例7岁以内脑瘫患儿随机分为治疗组和对照组,两组均接受常规穴位注射治疗和功能训练,治疗组在功能训练前或后施术点穴推拿、穴位注射,并在治疗前后进行综合评价,观察整体康复疗效,进行统计学处理.结果:治疗组整体疗效明显优于对照组(P<0.05).结论:点穴推拿和穴位注射为主的综合康复方法是治疗小儿脑瘫的最佳方法之一,是具有中国特色的综合康复体系,值得推行应用.%Purpose: To investigate therapeutic methods and results of comprehensive convalescent method of acupoint-pressing, Tuina, acupoint-injection and exercise in the treatment of childhood cerebral palsy. Methods: Six hundreds and forty children under 7 years old with cerebral palsy were divided randomly into treatment and control groups, and they were both given routine acupoint-injection and functional exercise, children in treatment groups were also given Tuina and acupoint-pressing therapies following acupoint-injection and functional exercise. Results: Therapeutic effect was significantly better in treatment group than that in control group (P< 0.05). Conclusion: Comprehensive convalescent method, mainly by acupointpress, Tuina, and acupoint-injection is one of premium therapy for treatment of childhood cerebral palsy, and this comprehensive convalescent therapy is really full of Chinese features and worthy of popularization.

  12. 温泉游泳训练对飞行员椎-基底动脉血流动力学影响的研究%Research on the effects of swimming training in thermal spring on pilots'basilar vertebral artery hemodynamic changes

    Institute of Scientific and Technical Information of China (English)

    王忠波; 李欣; 段虹; 吴燕红; 姚永杰; 李建香

    2011-01-01

    Objective To observe the effects of swimming training in thermal spring on pilots' basilar vertebral artery ( BA) hemo-dynamic changes during the convalescence. Methods Twenty four healthy male pilots in active service were selected as subjects. During the period of convalescence they were arranged to perform a four-week swimming training program ( 5days/week). Their ver-tebral artery hemodynamic changes before and after training were measured with transcranial Doppler ultrasound apparatus. Results After four-week swimming training in thermal water, pilots' posterior cerebral artery ( PC A) , vertebral artery ( VA) , basilar ver-tebral artery (BA) , peak systolic velocity ( Vs) , diastasis velocity ( Vd) , average maximum velocity ( Vm) , pulsatility index (PI) , resistive index (RI) were compared with pre-training, the difference showed statistical significance ( P < 0. 01). Conclu-sion During the convalescence, swimming training in thermal spring can increase the flow velocity and vascular compliance of pos-terior cerebral artery ( PCA) , vertebral artery ( VA) and basilar vertebral artery (BA) to improve the hemodynamic index.%目的 观察健康疗养期间,温泉游泳训练对飞行员椎动脉血流动力学指标的影响.方法 24名飞行员在健康疗养期间,进行连续4周,每周5d的温泉游泳训练.训练前后采用经颅多普勒超声仪检测椎动脉血流动力学指标.结果 与训练前相比,游泳训练4周后飞行员双侧大脑后动脉(PCA)、双侧大脑椎动脉(VA)、基底动脉(BA)、收缩期峰值血流速度(Vs)、舒张末期血流速度(Vd)、峰值平均血流速度(Vm),脉动指数(PI)、阻力指数(RI),训练后与训练前比较,差异有统计学意义(P<0.01).结论 健康疗养期间,温泉游泳训练可提高大脑后动脉、椎动脉、基底动脉的血流速度及血管顺应性,明显改善血流动力学指标.

  13. Molecular analysis of Shiga toxigenic Escherichia coli O111:H- proteins which react with sera from patients with hemolytic-uremic syndrome.

    Science.gov (United States)

    Voss, E; Paton, A W; Manning, P A; Paton, J C

    1998-04-01

    Western blot analysis was used to assess the reactivity of convalescent-phase sera from patients who were associated with an outbreak of hemolytic-uremic syndrome (HUS) caused by fermented sausage contaminated with Shiga toxin-producing Escherichia coli (STEC). The predominant STEC isolated from HUS patients belonged to serotype O111:H-, and reactivity to O111:H- whole-cell lysates, treated or untreated with proteinase K, was examined. As expected, all five serum samples demonstrated a marked anti-lipopolysaccharide response, but several protein bands were also immunoreactive, particularly one with an apparent size of 94 kDa. One convalescent-phase serum sample was subsequently used to screen an O111:H- cosmid bank and 2 of 900 cosmid clones were found to be positive, both of which contained a similar DNA insert. Western blot analysis of one of these clones identified three major immunoreactive protein bands of approximately 94, 70, and 50 kDa. An immune response to the three proteins was detectable with all five convalescent-phase serum samples but not with healthy human serum. Immunoreactive 94- and 50-kDa species were produced by a deletion derivative of the cosmid containing a 7-kb STEC DNA insert. Sequence analysis of this region indicated that it is part of the locus for enterocyte effacement, including the eaeA gene which encodes intimin. The deduced amino acid sequence of the O111:H- intimin was 88.6% identical to intimin from O157:H7 STEC, and the most divergent region was the 200 residues at the carboxyl terminus, which were only 75% identical. Such variation may be antigenically significant as serum from a HUS patient infected only with the O111:H- STEC reacted with intimin from an enteropathogenic E. coli O111 strain, as well as several other eaeA-positive STEC isolates, but not with an eaeA-positive STEC belonging to serotype O157:H-. Sera from two of the other HUS patients also failed to react with intimin from this latter strain. However, intimin

  14. Molecular Analysis of Shiga Toxigenic Escherichia coli O111:H− Proteins Which React with Sera from Patients with Hemolytic-Uremic Syndrome

    Science.gov (United States)

    Voss, Elena; Paton, Adrienne W.; Manning, Paul A.; Paton, James C.

    1998-01-01

    Western blot analysis was used to assess the reactivity of convalescent-phase sera from patients who were associated with an outbreak of hemolytic-uremic syndrome (HUS) caused by fermented sausage contaminated with Shiga toxin-producing Escherichia coli (STEC). The predominant STEC isolated from HUS patients belonged to serotype O111:H−, and reactivity to O111:H− whole-cell lysates, treated or untreated with proteinase K, was examined. As expected, all five serum samples demonstrated a marked anti-lipopolysaccharide response, but several protein bands were also immunoreactive, particularly one with an apparent size of 94 kDa. One convalescent-phase serum sample was subsequently used to screen an O111:H− cosmid bank and 2 of 900 cosmid clones were found to be positive, both of which contained a similar DNA insert. Western blot analysis of one of these clones identified three major immunoreactive protein bands of approximately 94, 70, and 50 kDa. An immune response to the three proteins was detectable with all five convalescent-phase serum samples but not with healthy human serum. Immunoreactive 94- and 50-kDa species were produced by a deletion derivative of the cosmid containing a 7-kb STEC DNA insert. Sequence analysis of this region indicated that it is part of the locus for enterocyte effacement, including the eaeA gene which encodes intimin. The deduced amino acid sequence of the O111:H− intimin was 88.6% identical to intimin from O157:H7 STEC, and the most divergent region was the 200 residues at the carboxyl terminus, which were only 75% identical. Such variation may be antigenically significant as serum from a HUS patient infected only with the O111:H− STEC reacted with intimin from an enteropathogenic E. coli O111 strain, as well as several other eaeA-positive STEC isolates, but not with an eaeA-positive STEC belonging to serotype O157:H−. Sera from two of the other HUS patients also failed to react with intimin from this latter strain. However

  15. Typhoid fever in young children in Bangladesh: clinical findings, antibiotic susceptibility pattern and immune responses.

    Directory of Open Access Journals (Sweden)

    Farhana Khanam

    2015-04-01

    Full Text Available Children bear a large burden of typhoid fever caused by Salmonella enterica serotype Typhi (S. Typhi in endemic areas. However, immune responses and clinical findings in children are not well defined. Here, we describe clinical and immunological characteristics of young children with S. Typhi bacteremia, and antimicrobial susceptibility patterns of isolated strains.As a marker of recent infection, we have previously characterized antibody-in-lymphocyte secretion (TPTest during acute typhoid fever in adults. We similarly assessed membrane preparation (MP IgA responses in young children at clinical presentation, and then 7-10 days and 21-28 days later. We also assessed plasma IgA, IgG and IgM responses and T cell proliferation responses to MP at these time points. We compared responses in young children (1-5 years with those seen in older children (6-17 years, adults (18-59 years, and age-matched healthy controls.We found that, compared to age-matched controls patients in all age cohorts had significantly more MP-IgA responses in lymphocyte secretion at clinical presentation, and the values fell in all groups by late convalescence. Similarly, plasma IgA responses in patients were elevated at presentation compared to controls, with acute and convalescent IgA and IgG responses being highest in adults. T cell proliferative responses increased in all age cohorts by late convalescence. Clinical characteristics were similar in all age cohorts, although younger children were more likely to present with loss of appetite, less likely to complain of headache compared to older cohorts, and adults were more likely to have ingested antibiotics. Multi-drug resistant strains were present in approximately 15% of each age cohort, and 97% strains had resistance to nalidixic acid.This study demonstrates that S. Typhi bacteremia is associated with comparable clinical courses, immunologic responses in various age cohorts, including in young children, and that TPTest

  16. Influencing Factors on Diabetic Complications of Type 2 Diabetes Cases%糖尿病疗养员并发症影响因素的调查分析

    Institute of Scientific and Technical Information of China (English)

    何丽英; 杨琳; 许望纯

    2011-01-01

    Objective To investigate the influencing factors on complications of type 2 diabetes convales-cents with a view to providing evidence for prevention and treatment of diabetic complications. Methods The data of 150 convalescents with type 2 diabetes were retrospectively analyzed on the blood glucose mo-nitoring, conditions of diabetes health education, outpatient follow-up period, blood glucose and complica-tions. Results The prevalence of type2 diabetes complications of convalescents were 61. 3%(92/150). The prevalence of diabetic complications were significantly influenced by proper dietary control, regular health education,outpatient following-up and frequent blood glucose monitor. Conclusion Proper diet control, regular health education,outpatient following-up on time and frequent blood glucose monitor can postpone type 2 diabetic complications effectively.%目的 分析2型糖尿病疗养员并发症的影响因素,以期为糖尿病并发症防治提供依据.方法 对广州军区广州疗养院150例2型糖尿病疗养员的血糖检测情况、接受糖尿病健康教育情况、门诊随访周期、血糖达标及并发症的临床资料进行回顾性统计分析.结果 150例疗养员中患有并发症者92例,占61.3%.饮食控制、健康教育、门诊随访、血糖检测频次对并发症的发生有显著影响(P<0.05).结论 合理饮食、加强血糖检测、定期接受健康教育、按时门诊随访能有效延缓2型糖尿病疗养员并发症的发生.

  17. The Influence of Ginkgo Terpene Lactones Meglumine Injection on Blood Hemorheology in Patients with Cerebral Infarction at Conva-lescence%银杏二萜内酯葡胺注射液对脑梗死恢复期患者血液流变学的影响

    Institute of Scientific and Technical Information of China (English)

    肖展翅; 倪小红; 李钢; 高聚; 陈洪汉

    2015-01-01

    Objective To investigate the effect of Ginkgo terpene lactones meglumine injection on blood hemorheology in patients with cerebral infarction at convalescence.Methods Sixty seven patients with cerebral infarction convalescence were randomly divided into two groups:The control group(n=33)treated with routine treatment,and the treatment group(n=34)treated with routine treat-ment plus Ginkgo terpene lactones meglumine injection for 2 weeks.The changes of blood rheology,nerve function defect score and side effects were observed after 2 weeks.Results After treatment,the changes of blood rheology and nerve function defect score in treatment group were better than that before treatment and in control group(P<0.05).Conclusion Ginkgo terpene lactones meglu-mine injection has good and safe effects in patients with cerebral infarction at convalescence.%目的:观察银杏二萜内酯葡胺注射液对动脉粥样硬化性血栓性脑梗死恢复期患者血液流变学的影响。方法将67例动脉粥样硬化性血栓性脑梗死恢复期患者随机分为两组。对照组33例,予西医常规治疗;治疗组34例,在对照组治疗的基础上加用银杏二萜内酯葡胺注射液静脉输注。两组均治疗2周。观察比较两组患者治疗前后血液流变学、神经功能缺损评分的变化,以及不良反应发生情况。结果治疗后,治疗组血液流变学及神经功能缺损评分改善优于治疗前与对照组(P<0.05),不良反应轻微。结论银杏二萜内酯葡胺注射液具有活血通络之功效,是治疗脑梗死安全有效的药物。

  18. Hemolytic Uremic Syndrome-associated Encephalopathy Successfully Treated with Corticosteroids.

    Science.gov (United States)

    Hosaka, Takashi; Nakamagoe, Kiyotaka; Tamaoka, Akira

    2017-09-25

    The encephalopathy that occurs in association with hemolytic uremic syndrome (HUS), which is caused by enterohemorrhagic Escherichia coli (E. coli), has a high mortality rate and patients sometimes present sequelae. We herein describe the case of a 20-year-old woman who developed encephalopathy during the convalescent stage of HUS caused by E.coli O26. Hyperintense lesions were detected in the pons, basal ganglia, and cortex on diffusion-weighted brain MRI. From the onset of HUS encephalopathy, we treated the patient with methylprednisolone (mPSL) pulse therapy alone. Her condition improved, and she did not present sequelae. Our study shows that corticosteroids appear to be effective for the treatment of some patients with HUS encephalopathy.

  19. Immunity against the GBV-B hepatitis virus in tamarins can prevent productive infection following rechallenge and is long-lived

    DEFF Research Database (Denmark)

    Bukh, Jens; Engle, Ronald E; Govindarajan, Sugantha;

    2008-01-01

    an infected tamarin, which was not infected with the related GBV-A viruses. Its infectivity titer was 10(6.6) tamarin 50% infectious doses per ml. Next, two tamarins that were convalescent from recombinant GBV-B infection were re-challenged. In the original infection viremia persisted for 8 and 12 weeks...... sequence of viruses recovered from both animals following the first re-challenge demonstrated that these did not represent immune escape variants since mutations were not detected. Neutralization studies suggested that the immunity was not humoral in nature. We also demonstrated that the immunity was long......GB virus-B (GBV-B) is the virus most closely related to hepatitis C virus (HCV). Thus, we have used GBV-B infection of tamarins, which develop acute hepatitis following experimental infection, as a surrogate model to study protective immunity. As challenge virus, we first produced a GBV-B pool from...

  20. The immunomodulatory effects of laparoscopic surgery.

    LENUS (Irish Health Repository)

    Corrigan, Mark

    2012-02-03

    Laparoscopic alternatives to conventional surgical procedures confer many advantages to patients including reduced postoperative pain, shortened convalescence and, perhaps, improved disease-related outcomes. The diminished degree of immune dysfunction apparent with these techniques may underpin these beneficial aspects. However, minimal access is accompanied by various ancillary anesthetic and mechanical associations (including the induction of a carbon dioxide pneumoperitoneum), which must be considered in addition to reduced tissue trauma when attempting to correlate cause with effect. Furthermore, the opportunity to establish causation between the immunomodulatory aspects of laparoscopy and subsequent clinical outcome by prospective, randomized study is difficult because of the rapid incorporation of minimal access techniques into clinical practice. Therefore, experimental in vitro and in vivo studies must be used to complement the limitations of clinical studies in this area. Although the initial investigations into the immunological effects of laparoscopy are encouraging, many of the intricacies associated with this approach still await elaboration.

  1. Improving Dengue Virus Capture Rates in Humans and Vectors in Kamphaeng Phet Province, Thailand, Using an Enhanced Spatiotemporal Surveillance Strategy

    Science.gov (United States)

    2015-05-18

    0.17) 0.202 Muscle or joint pain 3 (0.21) 36 (0.50) 0.077 Abdominal pain 2 (0.14) 15 (0.21) 0.727 Retro-orbital pain 3 (0.21) 22 (0.31) 0.749...4 (0.17) 0.101 Drowsiness 2 (0.09) 7 (0.23) 1 (0.10) 2 (0.09) 0.519 Muscle or joint pain 7 (0.32) 16 (0.52) 7 (0.70) 9 (0.39) 0.189 Abdominal ...village health worker over the 14-day period). If, between the day of enrollment and the day of convalescent blood sampling, the associate develops

  2. Charcot neuroarthropathy in simultaneous kidney–pancreas transplantation: report of two cases

    Directory of Open Access Journals (Sweden)

    Jorge Javier del Vecchio

    2013-08-01

    Full Text Available Charcot neuroarthropathy (CN is considered a major complication in diabetes mellitus (DM, and it is estimated that 1% of diabetic patients may develop this complication. Simultaneous kidney–pancreas transplantation (SKPT is one of the most effective therapies for patients with type 1 DM and end-stage diabetic nephropathy. Some cases with a Charcot-modified clinical presentation during the postoperative convalescence period after SKPT have been described. The clinical presentation may condition severe destructive lesions, and good practices include systematic follow-up. Based on the cases described, SKPT is one more entity that might lead to CN ‘foot-at-risk’. The aim of this article is to describe two cases of neuropathic arthropathy with rapid progression in the short term after SKPT.

  3. Expression and Purification of SARS Coronavirus Membrane Protein

    Institute of Scientific and Technical Information of China (English)

    戴五星; 雷明军; 吴少庭; 陈智浩; 梁靓; 潘晖榕; 秦莉; 高士同; 袁仕善; 张仁利

    2004-01-01

    To construct a recombinant plasmid Pet23a-M, the gene encoding severe acute respiratory syndrome (SARS) coronavirus membrane protein was amplified by RT-PCR and cloned into the expression plasmid Pet23a. Results of restriction endonuclease analysis, PCR detection and DNA sequencing analysis revealed that the cloned DNA sequence was the same as that reported. The re combinants were transformed into Escherichia coli (E. Coli) BL21 (DE3) and induced by Isopropylβ-D-thiogalactopyranoside (IPTG). The expression of 27 kD (1 kD=0. 992 1 ku) protein was detected by SDS-PAGE and pured by metal chelated chromatography. Results of Western-blot showed that this expressed protein could react with antibodies in sera of SARS patients during convalescence. This provided the basis for the further study on SARS virus vaccine and diagnostic agents.

  4. Use of serology and urine antigen detection to estimate the proportion of adult community-acquired pneumonia attributable to Streptococcus pneumoniae.

    Science.gov (United States)

    Watt, J P; Moïsi, J C; Donaldson, R L A; Reid, R; Ferro, S; Whitney, C G; Santosham, M; O'Brien, K L

    2010-12-01

    Streptococcus pneumoniae is a common cause of community-acquired pneumonia (CAP) but existing diagnostic tools have limited sensitivity and specificity. We enrolled adults undergoing chest radiography at three Indian Health Service clinics in the Southwestern United States and collected acute and convalescent serum for measurement of PsaA and PspA titres and urine for pneumococcal antigen detection. Blood and sputum cultures were obtained at the discretion of treating physicians. We compared findings in clinical and radiographic CAP patients to those in controls without CAP. Urine antigen testing showed the largest differential between CAP patients and controls (clinical CAP 13%, radiographic CAP 17%, control groups 2%). Serological results were mixed, with significant differences between CAP patients and controls for some, but not all changes in titre. Based on urine antigen and blood culture results, we estimated that 11% of clinical and 15% of radiographic CAP cases were due to pneumococcus in this population.

  5. Multimodal prophylaxis for venous thromboembolic disease after total hip and knee arthroplasty: current perspectives

    Institute of Scientific and Technical Information of China (English)

    LU Ning; Eduardo A. Salvati

    2010-01-01

    Life-threatening in the short term and leading to a high level of morbidity in the long term, venous thromboembolism (VTE) is the most fearful complication following lower limb arthroplasty. With advances in surgical procedure, anesthetic management and postoperative convalescence have altered the risks of venous thromboembolism after total joint arthroplasty in the lower extremity.The pathogenesis of VTE is multifactorial and includes the well-known Virchow's triad of hypercoagulability, venous stasis and endothelial damage. Therefore, it is appropriate to use a multimodal approach to thromboprophylaxis. Despite extensive research, the ideal multimodal prophylaxis against venous thrombolism has not been identified. So this article reviews the recent developments in multimodal prophylaxis for thromboembolism after total joint arthroplasty.

  6. Presence and Persistence of Zika Virus RNA in Semen, United Kingdom, 2016

    Science.gov (United States)

    Atkinson, Barry; Thorburn, Fiona; Petridou, Christina; Bailey, Daniel; Hewson, Roger; Simpson, Andrew J.H.; Brooks, Timothy J.G.

    2017-01-01

    Zika virus RNA has been detected in semen collected several months after onset of symptoms of infection. Given the potential for sexual transmission of Zika virus and for serious fetal abnormalities resulting from infection during pregnancy, information regarding the persistence of Zika virus in semen is critical for advancing our understanding of potential risks. We tested serial semen samples from symptomatic male patients in the United Kingdom who had a diagnosis of imported Zika virus infection. Among the initial semen samples from 23 patients, Zika virus RNA was detected at high levels in 13 (56.5%) and was not detected in 9 (39.1%); detection was indeterminate in 1 sample (4.4%). After symptomatic infection, a substantial proportion of men have detectable Zika virus RNA at high copy numbers in semen during early convalescence, suggesting high risk for sexual transmission. Viral RNA clearance times are not consistent and can be prolonged. PMID:27997333

  7. Preoperative dexamethasone improves surgical outcome after laparoscopic cholecystectomy: a randomized double-blind placebo-controlled trial

    DEFF Research Database (Denmark)

    Bisgaard, Thue; Klarskov, Birthe; Kehlet, Henrik

    2003-01-01

    were randomized to intravenous dexamethasone (8 mg) or placebo 90 minutes before LC. Patients received a similar standardized anesthetic, surgical, and multimodal analgesic treatment. All patients were recommended 2 days postoperative duration of convalescence. The primary endpoints were fatigue...... and pain. Preoperatively and at several times during the first 24 postoperative hours, we measured C-reactive protein (CRP) and pulmonary function, pain scores, nausea, and number of vomiting episodes were registered. Analgesic and antiemetic requirements were recorded. Also, on a daily basis, patients...... reported scores of fatigue and pain before and during the first postoperative week and the dates for resumption of work and recreational activities. RESULTS: Eight patients were excluded from the study, leaving 40 patients in each study group for analysis. There were no apparent side effects of the study...

  8. e-Justice Implementation at a National Scale: The Ugandan Case

    Science.gov (United States)

    Kitoogo, Fredrick Edward; Bitwayiki, Constantine

    The use of information and communications technologies has been identified as one of the means suitable for supplementing the various reforms in convalescing the performance of the justice sector. The Government of Uganda has made strides in the implementation of e-Government to effectively utilize information and communications technologies in governance. The justice players are manifested in a justice, law and order sector which is based on the the Sector Wide Approach whose basic principle is that communication, cooperation and coordination between institutions can greatly add value to service delivery within a sector. Although a subset of e-Government, e-Justice aims at improving service delivery and collaboration between all justice players through the use of ICTs and needs to be spear-headed at a sector level. This work proposes ways of harnessing the existing opportunities and methods to implement e-Justice in Uganda that will culminate into a generic framework that can be applied in similar countries.

  9. Incomplete Kawasaki disease associated with complicated Streptococcus pyogenes pneumonia: A case report.

    Science.gov (United States)

    Leahy, Timothy Ronan; Cohen, Eyal; Allen, Upton D

    2012-01-01

    A three-year-old boy presented with community-acquired pneumonia complicated by empyema. Streptococcus pyogenes (group A streptococcus) was identified on culture of the pleural fluid. The patient improved with antibiotic therapy and drainage of the empyema. During his convalescence, the patient developed persistent fever, lethargy and anorexia. His inflammatory markers were elevated, and repeat cultures were negative. Although the patient had none of the classical mucocutaneous features of Kawasaki disease, an echocardiogram was performed, which revealed coronary artery dilation. The patient was diagnosed with incomplete Kawasaki disease and treated with intravenous immunoglobulin and high-dose acetylsalicylic acid. The fever subsided within 48 h. To the authors' knowledge, the present report is the first report of Kawasaki disease associated with complicated S pyogenes pneumonia. It emphasizes the importance of considering incomplete Kawasaki disease among children with persistent fever, the role of echocardiography in diagnosis, and the potential link between Kawasaki disease and superantigen-producing organisms such as S pyogenes.

  10. Detection of hand, foot and mouth disease in the yucatan peninsula of Mexico.

    Science.gov (United States)

    Machain-Williams, Carlos; Dzul-Rosado, Alma R; Yeh-Gorocica, Aarón B; Rodriguez-Ruz, Katia G; Noh-Pech, Henry; Talavera-Aguilar, Lourdes; Salazar, Ma Isabel; Castro-Mussot, María Eugenia; Reyes-Solis, Guadalupe; Garcia-Rejon, Julián E; Puerto-Manzano, Fernando I; Blitvich, Bradley J

    2014-11-19

    We report a case of hand, foot and mouth disease (HFMD) in a 5-year-old male from Merida City in the Yucatan Peninsula of Mexico. A clinical and physical examination revealed that the patient had symptoms typical of HFMD, including fever, fatigue, odynophagia, throat edema, hyperemia, lesions on the hands and feet, and blisters in the oral cavity. The patient fully recovered after a convalescence period of almost three weeks. Reverse transcription-polymerase chain reaction and nucleotide sequencing revealed that the etiological agent was enterovirus 71 (EV71). The sequence has greatest (90.4%) nucleotide identity to the corresponding regions of EV71 isolates from the Netherlands and Singapore. Although HFMD is presumably common in Mexico, surprisingly there are no data in the PubMed database to support this. This case report provides the first peer-reviewed evidence of HFMD in Mexico.

  11. The identification of MacSe in Streptococcus equi ssp. equi

    Institute of Scientific and Technical Information of China (English)

    Jiande YANG; Yanfei LIU; Jun XU; Jifei MA

    2009-01-01

    Streptococcus equi subsp, equi (S. equi ssp. equi) causes equine strangles, a highly contagious and widespread purulent lymphadenitis of the head and neck. We have identified MacSe, a novel protein of S. equi, by screening a phage library of 3-8 kb random DNA fragments of S. equi CF32. MacSe shares 62% and 67.5% amino acid homology with Mac5005 and Mac8345 of S. pyogenes respectively. Expression during infection was shown by strong reactivity of the protein with convalescent sera and mucosal wash IgA of ponies infected by commingling exposure. Release into the culture medium was detected during the log phase of growth. Dose dependent anti-phagocytic activity for equine neutrophils involved interaction of MacSe with C3 and neutrophils.

  12. Prevention of postoperative ileus

    DEFF Research Database (Denmark)

    Holte, Kathrine; Kehlet, H

    2002-01-01

    Postoperative ileus (PI) is a major contributor to postoperative morbidity and prolonged convalescence after major surgical procedures. The pathophysiology of PI is multifactorial, including activation of the stress response to surgery, with inhibitory sympathetic visceral reflexes and inflammatory...... mediators. We update evidence on the advances in the prevention and treatment on PI. As single interventions, continuous thoracic epidural analgesia with local anesthetics and minimally invasive surgery are the most efficient interventions in the reduction of PI. The effects of pharmacological agents have...... generally been disappointing with the exception of cisapride and the introduction of the new selective peripherally acting m-opioid antagonists. Presently, introduction of a multi-modal rehabilitation programme (including continuous epidural analgesia with local anesthetics, early oral feeding and enforced...

  13. Danish Hernia Database recommendations for the management of inguinal and femoral hernia in adults

    DEFF Research Database (Denmark)

    Rosenberg, Jacob; Bisgaard, Thue; Kehlet, Henrik

    2011-01-01

    The nationwide Danish Hernia Database, recording more than 10,000 inguinal and 400 femoral hernia repairs annually, provides a unique opportunity to present valid recommendations in the management of Danish patients with groin hernia. The cumulated data have been discussed at biannual meetings...... and guidelines have been approved by the Danish Surgical Society. Diagnosis of groin hernia is based on clinical examination. Ultrasonography, CT or MRI are rarely needed, while herniography is not recommended. In patients with indicative symptoms of hernia, but no detectable hernia, diagnostic laparoscopy may......, using cost-effective local anaesthesia in open mesh repair and general anaesthesia for laparoscopic repair. Spinal anaesthesia is not recommended. Routine prophylactic antibiotics are not indicated. In the early convalescence period there are no physical restrictions. These guidelines will also...

  14. Detection of Hand, Foot and Mouth Disease in the Yucatan Peninsula of Mexico

    Science.gov (United States)

    Machain-Williams, Carlos; Dzul-Rosado, Alma R.; Yeh-Gorocica, Aarón B.; Rodriguez-Ruz, Katia G.; Noh-Pech, Henry; Talavera-Aguilar, Lourdes; Salazar, Ma. Isabel; Castro-Mussot, María Eugenia; Reyes-Solis, Guadalupe; Garcia-Rejon, Julián E.; Puerto-Manzano, Fernando I.; Blitvich, Bradley J.

    2014-01-01

    We report a case of hand, foot and mouth disease (HFMD) in a 5-year-old male from Merida City in the Yucatan Peninsula of Mexico. A clinical and physical examination revealed that the patient had symptoms typical of HFMD, including fever, fatigue, odynophagia, throat edema, hyperemia, lesions on the hands and feet, and blisters in the oral cavity. The patient fully recovered after a convalescence period of almost three weeks. Reverse transcription-polymerase chain reaction and nucleotide sequencing revealed that the etiological agent was enterovirus 71 (EV71). The sequence has greatest (90.4%) nucleotide identity to the corresponding regions of EV71 isolates from the Netherlands and Singapore. Although HFMD is presumably common in Mexico, surprisingly there are no data in the PubMed database to support this. This case report provides the first peer-reviewed evidence of HFMD in Mexico. PMID:25568757

  15. Detection of hand, foot and mouth disease in the Yucatan Peninsula of Mexico

    Directory of Open Access Journals (Sweden)

    Carlos Machain-Williams

    2014-12-01

    Full Text Available We report a case of hand, foot and mouth disease (HFMD in a 5-year-old male from Merida City in the Yucatan Peninsula of Mexico. A clinical and physical examination revealed that the patient had symptoms typical of HFMD, including fever, fatigue, odynophagia, throat edema, hyperemia, lesions on the hands and feet, and blisters in the oral cavity. The patient fully recovered after a convalescence period of almost three weeks. Reverse transcription-polymerase chain reaction and nucleotide sequencing revealed that the etiological agent was enterovirus 71 (EV71. The sequence has greatest (90.4% nucleotide identity to the corresponding regions of EV71 isolates from the Netherlands and Singapore. Although HFMD is presumably common in Mexico, surprisingly there are no data in the PubMed database to support this. This case report provides the first peer-reviewed evidence of HFMD in Mexico.

  16. [Unexplained right-sided heart failure following open heart surgery: mind the pericardium].

    Science.gov (United States)

    Swinkels, B M; Braam, R L; Rensing, B J W M; Jaarsma, W; Defauw, J J A M; Plokker, H W M

    2008-08-30

    Three men, aged 67 years, 80 years and 53 years, respectively, developed signs and symptoms of progressive right-sided heart failure following open heart surgery. They were diagnosed with constrictive pericarditis based on echocardiography, cardiac magnetic resonance and cardiac catheterisation. Following pericardiectomy, two of the patients fully recovered, while one, the 80-year-old man, died during convalescence. When signs and symptoms of progressive right-sided heart failure develop after open heart surgery, a diagnosis of constrictive pericarditis should be considered. Constrictive pericarditis after open heart surgery may be caused by inflammation of the pericardium; an old, fibrotic haemopericardium, which may be diffuse or loculated; pericardial adhesions; or a combination of these entities. Diagnosing constrictive pericarditis is difficult and may take a long time. However, it is important to recognise this disorder early before it has progressed to an advanced stage. Pericardiectomy is the only effective therapy. When performed too late, survival is significantly reduced.

  17. Replantation of an avulsive amputation of a foot after recovering the foot from the sea.

    Science.gov (United States)

    Yüksel, F; Karacaoğlu, E; Ulkür, E; Güler, M M

    2000-04-01

    A foot avulsion case, with the dismembered body part submerged in sea water for 1 hour, is presented. This report is unique in that it is the first to document the reattachment of a body part that had been submerged in sea water. It was not known how salt-water exposure would affect wound management. Differences in osmolarity and bacterial flora between the sea water and foot tissues have not caused any problems, and the patient has not suffered any vascular or infectious complications after replantation. Neurotization of the plantar surface by the tibial nerve, which was stripped off during amputation and replaced in its original traces, was the most critical part of convalescence. After management of such an interesting case, we conclude that exposure to sea water of the dismembered part should not be a contraindication for replantation surgery.

  18. Rapid eye movement-sleep is reduced in patients with acute uncomplicated diverticulitis—an observational study

    DEFF Research Database (Denmark)

    Huang, Chenxi; Alamili, Mahdi; Nielsen, Claus Henrik

    2015-01-01

    responses are believed to cause postoperative sleep disturbances, as inflammatory responses can alter sleep architecture through cytokine-brain interactions. Our aim was to investigate alteration of sleep architecture during acute infection and its relationships to inflammation and clinical symptoms......Introduction. Sleep disturbances are commonly found in patients in the postoperative period. Sleep disturbances may give rise to several complications including cardiopulmonary instability, transient cognitive dysfunction and prolonged convalescence. Many factors including host inflammatory....... Materials & Methods. In this observational study, we included patients with acute uncomplicated diverticulitis as a model to investigate the isolated effects of inflammatory responses on sleep. Eleven patients completed the study. Patients were admitted and treated with antibiotics for two nights, during...

  19. Flu Vaccination

    CERN Multimedia

    2006-01-01

    People working on the CERN site who wish to be vaccinated against influenza may go to the Medical Service (ground floor, Bldg. 57) without an appointment (preferably between 14:00 and 16:00), PROVIDED THAT THEY BRING THEIR OWN VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2006. The influenza vaccine is recommended for CERN staff aged 50 and over. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and for those convalescing from serious medical problems or major surgery. The Medical Service will not administer vaccines to family members or retired staff members, who must contact their family doctor. CERN Medical service

  20. Flu vaccination

    CERN Multimedia

    CERN Medical Service

    2006-01-01

    People working on the CERN site who wish to be vaccinated against influenza may go to the Medical Service (ground floor, Bldg. 57) without an appointment (preferably between 14:00 and 16:00), PROVIDED THAT THEY BRING THEIR OWN VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2006. The influenza vaccine is recommended for CERN staff aged 50 and over. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and for those convalescing from serious medical problems or major surgery. The Medical Service will not administer vaccines to family members or retired staff members, who must contact their family doctor.CERN Medical Service

  1. Flu Vaccination

    CERN Document Server

    2006-01-01

    People working on the CERN site who wish to be vaccinated against influenza may go to the Medical Service (ground floor, Bldg. 57) without an appointment (preferably between 14:00 and 16:00), PROVIDED THAT THEY BRING THEIR OWN VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2006. The influenza vaccine is recommended for CERN staff aged 50 and over. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and for those convalescing from serious medical problems or major surgery. The Medical Service will not administer vaccines to family members or retired staff members, who must contact their family doctor. CERN Medical Service

  2. Note from the CERN Medical Service

    CERN Multimedia

    2005-01-01

    FLU VACCINATION People working on the CERN site who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment (preferably between 14:00 and 16:00), but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2005. CERN staff aged 50 or over are recommended to have the flu vaccination. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious illness or after major surgery. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their usual family doctor.

  3. Note from the CERN Medical Service

    CERN Multimedia

    Medical Service

    2004-01-01

    FLU VACCINATION People working on the CERN site who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2004. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  4. FLU VACCINATION

    CERN Document Server

    2007-01-01

    People working on the CERN site who wish to be vaccinated may go to the Infirmary (ground-floor, bldg. 57), with their vaccine, without a prior appointment. The vaccine can be reimbursed directly by Uniqa providing you attach the receipt and the prescription that you will receive from the Medical Service the day of your injection at the infirmary. Ideally, the vaccination should take place between 1st October and 30th November 2007 (preferably between 14:00 and 16:00). CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor. Medical Service

  5. FLU VACCINATION

    CERN Multimedia

    2006-01-01

    People working on the CERN site who wish to be vaccinated against influenza may go to the Medical Service (ground floor, Bldg. 57) without an appointment (preferably between 14:00 and 16:00), PROVIDED THAT THEY BRING THEIR OWN VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2006. The influenza vaccine is recommended for CERN staff aged 50 and over. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and for those convalescing from serious medical problems or major surgery. The Medical Service will not administer vaccines to family members or retired staff members, who must contact their family doctor. CERN Medical Service

  6. Note from the CERN Medical Service - FLU VACCINATION

    CERN Document Server

    2007-01-01

    People working on the CERN site who wish to be vaccinated may go to the infirmary (ground-floor, bldg. 57), with their vaccine without a prior appointment. The reimbursement of the vaccine can be done directly with Uniqa providing you attach the receipt and the prescription that you will receive from the medical service the day of your injection at the infirmary. Ideally, the vaccination should take place between 1st October and 30th November 2007 (preferably between 14:00 and 16:00). CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  7. Note from the CERN Medical Service FLU VACCINATION

    CERN Document Server

    2007-01-01

    People working on the CERN site who wish to be vaccinated may go to the infirmary (ground-floor, bldg. 57), with their vaccine without a prior appointment. The reimbursement of the vaccine can be done directly with Uniqa providing you attach the receipt and the prescription that you will receive from the medical service the day of your injection at the infirmary. Ideally, the vaccination should take place between 1st October and 30th November 2007 (preferably between 14:00 and 16:00). CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  8. Note from the CERN Medical Service

    CERN Multimedia

    Medical Service

    2005-01-01

    FLU VACCINATION People working on the CERN site who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2006. CERN staff aged 50 or over are recommended to have the flu vaccination.  Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious illness or after major surgery . The Medical Service will not administer vaccines for family members or retired staff members, who must contact their usual family doctor. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  9. Note from the CERN Medical Service FLU VACCINATION

    CERN Multimedia

    2007-01-01

    People working on the CERN site who wish to be vaccinated may go to the infirmary (ground-floor, bldg. 57), with their vaccination without a prior appointment. The reimbursement of the vaccination can be done directly with Uniqa providing you attach the receipt and the prescription that you will receive from the medical service the day of your injection at the infirmary. Ideally, the vaccination should take place between 1st October and 30th November 2007 (preferably between 14:00 and 16:00). CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  10. Broadly Neutralizing Activity of Zika Virus-Immune Sera Identifies a Single Viral Serotype

    Directory of Open Access Journals (Sweden)

    Kimberly A. Dowd

    2016-08-01

    Full Text Available Recent epidemics of Zika virus (ZIKV have been associated with congenital malformation during pregnancy and Guillain-Barré syndrome. There are two ZIKV lineages (African and Asian that share >95% amino acid identity. Little is known regarding the ability of neutralizing antibodies elicited against one lineage to protect against the other. We investigated the breadth of the neutralizing antibody response following ZIKV infection by measuring the sensitivity of six ZIKV strains to neutralization by ZIKV-confirmed convalescent human serum or plasma samples. Contemporary Asian and early African ZIKV strains were similarly sensitive to neutralization regardless of the cellular source of virus. Furthermore, mouse immune serum generated after infection with African or Asian ZIKV strains was capable of neutralizing homologous and heterologous ZIKV strains equivalently. Because our study only defines a single ZIKV serotype, vaccine candidates eliciting robust neutralizing antibody responses should inhibit infection of both ZIKV lineages, including strains circulating in the Americas.

  11. Randomization to nutritional intervention at home did not improve postoperative function, fatigue or well-being

    DEFF Research Database (Denmark)

    Jensen, Martin Bach; Hessov, Ib

    1997-01-01

    BACKGROUND: Postoperative fatigue and deterioration in functional capacity have been correlated to postoperative weight loss. This suggested that nutritional support to enhance the regain of weight might improve upon the convalescence. METHODS: Patients were allocated randomly at discharge......, gain in body-weight and lean body mass, especially in the legs as shown by dual-energy X-ray absorptiometry. Changes in work capacity, hand grip and pinching strength, fatigue and quality of life were similar in patients in control and intervention groups and had returned to preoperative values 1......-2 months after operation, although fatigue was increased at 2-3 months. CONCLUSIONS: This study did not support the hypothesis that changes in weight were important for the changes in function and fatigue in patients with small to moderate weight loss....

  12. A case of benign acute childhood myositis associated with influenza A (H1N1) virus infection.

    Science.gov (United States)

    Koliou, M; Hadjiloizou, S; Ourani, S; Demosthenous, A; Hadjidemetriou, A

    2010-02-01

    Benign acute childhood myositis (BACM) is a rare transient condition usually occurring at the early convalescent phase of a viral upper respiratory tract illness, normally influenza A, and, more frequently, influenza B infection. It is characterized by acute-onset difficulty in walking as a result of severe bilateral calf pain and by elevated muscle enzymes including creatinine kinase. It is self-limiting because there is rapid full recovery usually within 1 week. We describe the first case of BACM in association with the new pandemic influenza A (H1N1) virus infection in an 11-year-old boy from Cyprus. The child had the typical clinical and laboratory characteristics of this clinical syndrome. Prompt diagnosis of this clinical entity is essential to prevent unnecessary investigations and therapeutic interventions and to reassure the patient and parents of the excellent prognosis.

  13. Benign Acute Childhood Myositis During Influenza B Outbreak.

    Science.gov (United States)

    Szenborn, Leszek; Toczek-Kubicka, K; Zaryczański, J; Marchewka-Kowalik, M; Miśkiewicz, K; Kuchar, E

    2017-08-10

    Benign acute childhood myositis (BACM) is a syndrome classically occurring in children during the convalescent phase from a febrile upper respiratory tract infection, most commonly after influenza B. BACM can cause difficulty walking due to severe calf pain. Laboratory results show increased serum creatinine kinase and AST. Although alarming, BACM is self-limiting with symptoms disappearing within a week. Herein, we described a case series of BCAM in children in two cities in Poland during the influenza outbreaks in 2012/2013 and 2014/2015. We discussed the presentation and the clinical workup and examinations of the myositic syndrome. In addition, we evaluated the association of BACM with influenza B. We detected specific IgG against influenza B virus in 83% of the children diagnosed with BCAM. Reports from the National Institute of Public Health - National Institute of Hygiene in Warsaw, Poland confirmed a high rate of influenza B cases during both epidemic seasons in question.

  14. Feasibility of Xpert Ebola Assay in Médecins Sans Frontières Ebola Program, Guinea.

    Science.gov (United States)

    Van den Bergh, Rafael; Chaillet, Pascale; Sow, Mamadou Saliou; Amand, Mathieu; van Vyve, Charlotte; Jonckheere, Sylvie; Crestani, Rosa; Sprecher, Armand; Van Herp, Michel; Chua, Arlene; Piriou, Erwan; Koivogui, Lamine; Antierens, Annick

    2016-02-01

    Rapid diagnostic methods are essential in control of Ebola outbreaks and lead to timely isolation of cases and improved epidemiologic surveillance. Diagnosis during Ebola outbreaks in West Africa has relied on PCR performed in laboratories outside this region. Because time between sampling and PCR results can be considerable, we assessed the feasibility and added value of using the Xpert Ebola Assay in an Ebola control program in Guinea. A total of 218 samples were collected during diagnosis, treatment, and convalescence of patients. Median time for obtaining results was reduced from 334 min to 165 min. Twenty-six samples were positive for Ebola virus. Xpert cycle thresholds were consistently lower, and 8 (31%) samples were negative by routine PCR. Several logistic and safety issues were identified. We suggest that implementation of the Xpert Ebola Assay under programmatic conditions is feasible and represents a major advance in diagnosis of Ebola virus disease without apparent loss of assay sensitivity.

  15. Late-stage Use of Low-dose Corticosteroids Aid Recovery of Severe H1N1 Viral Pneumonia

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    The role of corticosteroids in the management of severely ill patients with inlfuenza A (H1N1) viral infection is unclear and controversial. Two critically ill cases with influenza A (H1N1) infections complicated with organizing pneumonia (OP) in 2011 successfully treated with low dose corticosteroids were reported here. After initial clinical improvement, the condition of both patients aggravated 20-23 days after the onset of illness. Chest X-ray and computed tomographies (CT) showed an increment of lung infiltrates. Cultures of blood, pleural lfuid and transbronchial aspirate were negative for bacteria and fungi. Organizing pneumonia was diagnosed clinically and both patients were successfully treated with low-dose corticosteroids. Low-dose corticosteroids initiated during convalescence may be beneficial for severe swine-origin influenza A H1N1 pandemic 2009 virus (S-OIV) infections.

  16. Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance

    Science.gov (United States)

    Hsu, Alan Yi-Hui; Wu, Shang-Rung; Tsai, Jih-Jin; Chen, Po-Lin; Chen, Ya-Ping; Chen, Tsai-Yun; Lo, Yu-Chih; Ho, Tzu-Chuan; Lee, Meed; Chen, Min-Ting; Chiu, Yen-Chi; Perng, Guey Chuen

    2015-01-01

    The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a “sunny-side up egg” appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development. PMID:26657027

  17. Robot-assisted partial nephrectomy in contemporary practice

    Directory of Open Access Journals (Sweden)

    Youssef S. Tanagho

    2013-01-01

    Full Text Available Laparoscopic renal surgery is associated with reduced blood loss, shorter hospital stay, enhanced cosmesis, and more rapid convalescence relative to open renal surgery. Laparoscopic partial nephrectomy is a minimally invasive, nephron-sparing alternative to laparoscopic radical nephrectomy for the management of small renal masses. While offering similar oncological outcomes to laparoscopic radical nephrectomy, the technical challenges and prolonged learning curve associated with laparoscopic partial nephrectomy limit its wider dissemination. Robot-assisted partial nephrectomy, although still an evolving procedure with no long-term data, has emerged as a viable alternative to laparoscopic partial nephrectomy, with favorable preliminary outcomes. This article provides an overview of the role of robot-assisted partial nephrectomy in the management of renal cell carcinoma. The clinical indications and principles of surgical technique for this procedure are discussed. The oncological, renal functional, and perioperative outcomes of robot-assisted partial nephrectomy are also evaluated, as are complication rates.

  18. 狼疮性肾炎患者血清IL-6、sIL-2R水平测定

    Institute of Scientific and Technical Information of China (English)

    胡波; 罗敏琪; 粱家隐; 叶少雄; 李朝霞

    2004-01-01

    To investigate the Changes of sIL - 2R, IL - 6 level in ,serum of patients with lupus nephritis and its clinical meaning. Methods The level of serum sIL - 2R, IL - 6 in 42 cases of lupus nephritis were detected by ELISA, and the data were compared with control group. Results Serum sIL- 2R ,IL-6 levels in lupus nephritis patients were significantly higher than control group and related to convalescent of the disease. Conclusion It suggested that immune dysfunction exist in patients with lupus nephritis higher level of sIL-2 ,IL-6 would associate with genesis and development of lupus nephritis, and it can be a new indicator of lupus nephritis.

  19. Vascular Endothelial Growth Factor Levels in Dobrava/Belgrade Virus Infections

    Directory of Open Access Journals (Sweden)

    Katerina Tsergouli

    2013-12-01

    Full Text Available The levels of vascular endothelial growth factor-A (VEGF were estimated in 102 serum samples from 63 hospitalized Greek patients with hemorrhagic fever with renal syndrome (HFRS caused by Dobrava/Belgrade virus. Significantly higher VEGF levels were seen in the severe when compared with non-severe cases (mean values 851.96 pg/mL and 326.75 pg/mL, respectively; p = 0.003, while a significant difference was observed among groups based on the day after the onset of illness. In both severe and non-severe cases, VEGF peaked in the second week of illness; however, elevation of VEGF in the severe cases started later and remained high until convalescence, suggesting that the role of VEGF was associated with repair of vascular damage rather than with increased permeability.

  20. Vascular endothelial growth factor levels in dobrava/belgrade virus infections.

    Science.gov (United States)

    Tsergouli, Katerina; Papa, Anna

    2013-12-10

    The levels of vascular endothelial growth factor-A (VEGF) were estimated in 102 serum samples from 63 hospitalized Greek patients with hemorrhagic fever with renal syndrome (HFRS) caused by Dobrava/Belgrade virus. Significantly higher VEGF levels were seen in the severe when compared with non-severe cases (mean values 851.96 pg/mL and 326.75 pg/mL, respectively; p = 0.003), while a significant difference was observed among groups based on the day after the onset of illness. In both severe and non-severe cases, VEGF peaked in the second week of illness; however, elevation of VEGF in the severe cases started later and remained high until convalescence, suggesting that the role of VEGF was associated with repair of vascular damage rather than with increased permeability.

  1. Dengue NS1 and prM antibodies increase the sensitivity of acute dengue diagnosis test and differentiate from Japanese encephalitis infection.

    Science.gov (United States)

    Gowri Sankar, S; Balaji, T; Venkatasubramani, K; Thenmozhi, V; Dhananjeyan, K J; Paramasivan, R; Tyagi, B K; John Vennison, S

    2014-05-01

    Accurate and early diagnosis of dengue infection is essential for dengue case management. In outbreak conditions, it is essential to include two different tests to diagnose dengue and the choice depends on the number of days after the onset of illness in which the sample is collected. During the laboratory diagnosis of dengue in late acute and convalescent phase by MAC-ELISA, it is necessary to rule out possible cross reactions of closely related flavivirus, such as Japanese encephalitis virus which is commonly co-circulating. In the present investigation, the usefulness of dengue virus NS1 and prM antibodies in diagnosing and differentiating dengue from Japanese encephalitis infection was assessed using samples collected during out-breaks. It was shown here that, detection of antibodies against dengue NS1 and prM proteins increases the sensitivity of dengue diagnosis until 15days. Moreover, detection of antibodies against both proteins was able to differentiate dengue from Japanese encephalitis infection.

  2. Proposed application of lower body negative pressure to cardiology

    Science.gov (United States)

    Schmidt, E. V.; Debusk, R. F.; Popp, R. L.

    1975-01-01

    Potential medical applications are presented of lower body negative pressure to the evaluation and treatment of cardiac patients. The essential features of an LBNP unit and the basic cardiovascular physiology of lower body negative pressure (LBNP) testing are described. Some of the results of previous spaceflight experiences and bedrest studies are summarized. The deconditioning effects of weightlessness experienced by orbiting astronauts are compared with the effects of bedrest restrictions prescribed for convalescing cardiac patients. The potential of LBNP for evaluating both pharmacological and physical activity regimens was examined, particularly in relation to post-myocardial infarction and coronary artery bypass patients. Applications of LBNP to the cardiac catheterization laboratory and the out-patient follow-up of cardiac patients are proposed.

  3. CLINICAL ANALYSIS ON THE TREATMENT OF FACIAL PARALYSIS BY ACUPUNCTURE, MOXIBUSTION PLUS CHINESE HERBAL MEDICINES

    Institute of Scientific and Technical Information of China (English)

    ZHANG Li-sha; SHEN Wei-hong

    2005-01-01

    Objective:To observe the therapeutic effect of the integrated acupuncture-moxibustion and herbal therapies for facial paralysis. Methods: A total of 68 cases of facial paralysis were treated by integration of acupuncture-moxibustion and herbal therapies. For facial palsy patients with wind-phlegm blocking meridian-collaterals and wind-stirring due to yin deficiency types in the acute stage, Modified respectively; and for those in convalescent stage and sequela stage, Replenishing Blood) + Qian Zheng San (Powder for Treating Wry-mouth) and Recuperation) were employed. Results: After the treatment, the curative rate of the 68 cases accounted for 90% of the total cases, the remarkably effective 6%, the improved 3% and the poor 1% respectively. Conclusion: The therapeutic effectiveness of the integrated treatment of acupuncture-moxibustion and herbal therapies for facial paralysis is certain.

  4. Reduced erythrocyte deformability associated with hypoargininemia during Plasmodium falciparum malaria.

    Science.gov (United States)

    Rey, Juliana; Buffet, Pierre A; Ciceron, Liliane; Milon, Geneviève; Mercereau-Puijalon, Odile; Safeukui, Innocent

    2014-01-20

    The mechanisms underlying reduced red blood cell (RBC) deformability during Plasmodium falciparum (Pf) malaria remain poorly understood. Here, we explore the possible involvement of the L-arginine and nitric oxide (NO) pathway on RBC deformability in Pf-infected patients and parasite cultures. RBC deformability was reduced during the acute attack (day0) and returned to normal values upon convalescence (day28). Day0 values correlated with plasma L-arginine levels (r = 0.69; p = 0.01) and weakly with parasitemia (r = -0.38; p = 0.006). In vitro, day0 patient's plasma incubated with ring-stage cultures at 41°C reduced RBC deformability, and this effect correlated strongly with plasma L-arginine levels (r = 0.89; p falciparum malaria may altogether impair NO production and reduce RBC deformability, particularly at febrile temperature.

  5. Flu Vaccination

    CERN Multimedia

    2006-01-01

    People working on the CERN site who wish to be vaccinated against influenza may go to the Medical Service (ground floor, Bldg. 57) without an appointment (preferably between 14:00 and 16:00), PROVIDED THAT THEY BRING THEIR OWN VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2006. The influenza vaccine is recommended for CERN staff aged 50 and over. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and for those convalescing from serious medical problems or major surgery. The Medical Service will not administer vaccines to family members or retired staff members, who must contact their family doctor. CERN Medical service

  6. Note from the CERN Medical Service

    CERN Multimedia

    2005-01-01

    FLU VACCINATION People working on the CERN site who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment (preferably between 14:00 and 16:00), but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2005. CERN staff aged 50 or over are recommended to have the flu vaccination. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious illness or after major surgery. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their usual family doctor.

  7. Note from the CERN Medical Service

    CERN Multimedia

    Medical Service

    2004-01-01

    FLU VACCINATION People working on the CERN site who wish to be vaccinated may go to the Medical Service (ground-floor, bldg. 57), without a prior appointment, but THEY MUST BRING THEIR VACCINE WITH THEM. Ideally, vaccination should take place between 1st October and 30th November 2004. CERN staff aged 50 or over are recommended to have influenza vaccinations. Vaccination is particularly important for those suffering from chronic lung, cardio-vascular or kidney problems, for diabetics and those convalescing from serious medical problems or after serious surgical operations. The Medical Service will not administer vaccines for family members or retired staff members, who must contact their normal family doctor.

  8. Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance.

    Science.gov (United States)

    Hsu, Alan Yi-Hui; Wu, Shang-Rung; Tsai, Jih-Jin; Chen, Po-Lin; Chen, Ya-Ping; Chen, Tsai-Yun; Lo, Yu-Chih; Ho, Tzu-Chuan; Lee, Meed; Chen, Min-Ting; Chiu, Yen-Chi; Perng, Guey Chuen

    2015-12-11

    The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a "sunny-side up egg" appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.

  9. Compensation neurosis rides again.

    Science.gov (United States)

    Levy, A

    1992-01-01

    Compensation neurosis (CN), also known as accident neurosis, has generally not been considered to be a 'real' disorder. In 1961 it was seemingly laid to rest by Henry Miller, a distinguished neurologist, in a sharp article which appeared in the British Medical Journal. Miller's view of patients who presented psychological symptoms following accidents or traumas was suspicious. Compensated or not, his view seemed to be that they should have their legal process finished as quickly as possible and then they will miraculously convalescence. Miller's work, it appeared, was the coup de grâce for this ill-defined diagnosis. Today, however, compensation neurosis seems to ride again. After a prolonged silence in the psychiatric literature, new papers are emerging, strongly suggesting that this vanishing diagnosis be reconsidered. This new trend will be presented.

  10. Model of Property Services Team in Hospitalized Patients with Chronic Mental Illness%住院慢性精神病患者开展物业服务队模式研究

    Institute of Scientific and Technical Information of China (English)

    盛嘉玲; 朱岚; 张建华; 顾燕; 吕珍术; 俞左英

    2011-01-01

    目的:探讨住院慢性精神病患者开展院内物业服务队的康复模式,寻找恢复期慢性精神病患者职业康复的新途径.方法:101例恢复期慢性精神病患者分为物业组50例和对照组51例,均用精神病药物维持治疗.物业组开展理发、洗衣、商店、保洁及维修等类似物业的岗位训练,采用全开放和半开放管理方法.于入组前、治疗后3、6、12个月末时对2组患者进行阳性和阴性症状量表(PANSS)和住院精神病患者康复疗效评定量表(IPROS)评定.结果:物业组在治疗3个月末时,PANSS中阴性症状及精神病理单因子评分均明显低于对照组;6及12个月末时下降更显著,PANSS总分也出现明显下降(P92%,劳动态度和劳动质量均随时间的推移而提高.结论:院内物业服务队可成为恢复期慢性精神病患者新的职业康复途径,但让其回归社会还需要多渠道合作.%Objective: To investigate the rehabilitation model of hospital property services team in the convalescent inpatients with chronic mental illness, and find new ways to vocational rehabilitation of the inpatients with convalescent chronic mental illness.Methods.: 101 convalescent patients with chronic mental illness were divided into the property group (n= 50) and control group (nn= 51), and treated with psychiatric drugs for maintenance The patients in property group had the training of hairdressing, laundry, shopping, cleaning and repair, etc.The effects of one-year rehabilitation were observed by using all open and semi-open management methods.Results: The negative symptoms and psychopathology scores from the positive and negative syndrome scale (PANSS) in property group were significantly lower than in control group at the end of 3rd month after treatment, more significantly at the end of 6th and 12th month.The total PANSS scores were also obviously declined.The life ability and regard and interest scores from Inpatient Psychiatric Rehabilitation

  11. Statistical and Multidimensional Body Composition Parameter Analysis in Young Childhood Cancer Survivors

    Directory of Open Access Journals (Sweden)

    Topczewska Magdalena

    2014-12-01

    Full Text Available This article concerns the problem of assessing selected body compo- sition parameters after completion of antitumor therapy and comparing them with the same parameters of healthy children. A high percentage of overweight and obesity, as well as abnormal fat distribution in convalescents with cancer shows a significant adverse effect of therapy on body composition and suggests the need for early intervention in terms of diet and exercise, which would help patients to quickly achieve the proper parameters of body composition. Two main problems will be mentioned during the presented data analysis. Firstly, in each group there was a small number of observations. Because of this, the real differences between examined subgroups may have been omitted. Secondarily, many variables are correlated and are not normally distributed. Therefore, be- side the standard statistical tests to compare two groups, principal component analysis was applied to reduce the dimensions of the attribute space and to attempt to classify two groups of patients.

  12. HOSPITAL EXPERIENCE WITH CARDIAC RESUSCITATION.

    Science.gov (United States)

    CASDORPH, H R

    1964-04-01

    In three cases of cardiac arrest in which resuscitation was successful, there were striking similarities. The patients were men with anterior myocardial infarction and the mode of cardiac arrest was ventricular fibrillation. Corrective action was begun promptly after arrest occurred. In each case vasopressor agents were required for three to five days after resuscitation before the cardiovascular system was able to maintain a normal blood pressure without this aid. Convalescence was satisfactory and each patient returned to his usual occupation. The procedure that was used for cardiac resuscitation was based on artifical respiration (by any of several methods) and artificial circulation by external cardiac compression to provide a flow of oxygenated blood to the brain. Once this is established, time is afforded to determine what other steps are needed.

  13. A STUDY ON DETECTION OF SERUMFASTING TOTAL BILE ACID AND CHOLOYGLYCIN IN NEONATE FOR CHOLESTASIS

    Institute of Scientific and Technical Information of China (English)

    郭文; 吴明昌; 裴学义; 关德华; 徐洛

    1996-01-01

    Enzyme-linked colorimetric analysis and radioimmunossay were employed to detect serum fasting total bile acids(FBA) and choloyglyein (CG) respectively in eases of 32 neonatal hepatitis, 33 cases of neona-tal uneonjugated hyperbilirubinemia and 34 cases of breast milk jaundice(BMJ). FBA and CG in acute period of neonatal hepatitis were obviously elevated and decreased gradually in convalescent and recovered period. The difference was significant for each stage as compared with controls. Acute FBA correlated strongly with the course. Both neonatal unconjugated hyperbilirubinemia and BMJ differed significantlyfrom controls in FBA and CG. The results suggested that serum FBA and CGr were helpful in judging the course and state of neonatal hepatitis, and eholestasls might existed in neonatal unconjngated hyperbilirubinemia.

  14. Posttreatment Lyme disease syndrome.

    Science.gov (United States)

    Aucott, John N

    2015-06-01

    The prognosis following appropriate antibiotic treatment of early or late Lyme disease is favorable but can be complicated by persistent symptoms of unknown cause termed posttreatment Lyme disease syndrome (PTLDS), characterized by fatigue, musculoskeletal pain, and cognitive complaints that persist for 6 months or longer after completion of antibiotic therapy. Risk factors include delayed diagnosis, increased severity of symptoms, and presence of neurologic symptoms at time of initial treatment. Two-tier serologic testing is neither sensitive nor specific for diagnosis of PTLDS because of variability in convalescent serologic responses after treatment of early Lyme disease. Optimal treatment of PTLDS awaits more precise understanding of the pathophysiologic mechanisms involved in this illness and future treatment trials. Copyright © 2015 Elsevier Inc. All rights reserved.

  15. Characterization and immunogenicity of Kingella kingae outer-membrane proteins.

    Science.gov (United States)

    Yagupsky, Pablo; Slonim, Ariela

    2005-01-01

    In recent years, Kingella kingae has emerged as an important pediatric pathogen but the antigenicity of the organism and the host immune response have not been studied. Outer membrane proteins (OMPs) of 57 K. kingae isolates were characterized and the immune response of 19 children with invasive infections was studied by immunoblotting. Kingella kingae OMPs were remarkably similar disregarding place and time of isolation and associated clinical condition (asymptomatic carriage, bacteremia, endocarditis, septic arthritis or osteomyelitis). Most OMPs were immunogenic but the specific bands that reacted in each strain and the intensity of the reactions varied substantially. When convalescent sera were reacted with heterologous strains, bands that either were not recognized by the homologous serum or were not present in the homologous strain were visualized. These results demonstrate that OMPs of K. kingae are highly conserved but suggest that some epitopes are polymorphic, resulting in a variable pattern of immune response.

  16. COMBINED TREATMENT OF 200CASES OF PERIPHERY FACIAL PARALYSIS WITH ACUPUNCTURE AND CHINESE MEDICINAL HERBS

    Institute of Scientific and Technical Information of China (English)

    LuoHeping

    2000-01-01

    In the present study,200cases of periphery facial paralysis were treated with combined therapies of handle needle,electroacupuncture(EA),electronic moxibustion and oral administration of Supplemented Qianzheng San.After treatment,of the200cases,176were cured,15had marked im-provement and 9had improvement.Two groups of acupoints were chosen and used alternately for keeping the excitement of acupoints,preventing acupoint fatigue and raising the therapeutic effect.In the acute stage of periphery facial palsy,acupuncture treatment was performed using shallow needing,mild manipulation and no needing penetration.In the convalescence stage and sequela stage,penetra-tion needling was adopted and stronger stimulation given.During acute period and when facial muscu-lar spasm appeared,EA stimulation was not suitable for facial acupoints.Over use of vision should be avoided.

  17. Research Progress of Natural Therapy for Disposition of Healthful Plants%自然疗法用于植物景观配置的研究进展

    Institute of Scientific and Technical Information of China (English)

    刘丽

    2012-01-01

    文章介绍了自然疗法的定义、植物的保健功效及其在景观设计中的三种应用方式。并从视觉、听觉、嗅觉、触觉四方面功效选择保健型植物来构建康复疗养型景观的问题进行了研究和论述。%Be based on the introduction of nature therapy's definition and connotation, the health care function of plant and three kinds' applications of the healthful plants within landscape design have been introduced. In convalescing health care landscape, how to select plant species and deploy plant community from visual, audible, smelling and touch four aspects. Also introduces some harmful plants and flowers.

  18. Development of a Summarized Health Index (SHI) for use in predicting survival in sea turtles.

    Science.gov (United States)

    Li, Tsung-Hsien; Chang, Chao-Chin; Cheng, I-Jiunn; Lin, Suen-Chuain

    2015-01-01

    Veterinary care plays an influential role in sea turtle rehabilitation, especially in endangered species. Physiological characteristics, hematological and plasma biochemistry profiles, are useful references for clinical management in animals, especially when animals are during the convalescence period. In this study, these factors associated with sea turtle surviving were analyzed. The blood samples were collected when sea turtles remained alive, and then animals were followed up for surviving status. The results indicated that significantly negative correlation was found between buoyancy disorders (BD) and sea turtle surviving (p sea turtles had significantly higher levels of aspartate aminotranspherase (AST), creatinine kinase (CK), creatinine and uric acid (UA) than surviving sea turtles (all p sea turtles and to improve veterinary care at rehabilitation facilities.

  19. Presumed appendiceal abscess discovered to be ruptured Meckel diverticulum following percutaneous drainage

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Jeannie C.; Ostlie, Daniel J. [Children' s Mercy Hospital, Department of Surgery, Kansas City, MO (United States); Rivard, Douglas C.; Morello, Frank P. [Children' s Mercy Hospital, Department of Radiology, Kansas City, MO (United States)

    2008-08-15

    A Meckel diverticulum is an embryonic remnant of the omphalomesenteric duct that occurs in approximately 2% of the population. Most are asymptomatic; however, they are vulnerable to inflammation with subsequent consequences including diverticulitis and perforation. We report an 11-year-old boy who underwent laparoscopic appendectomy for perforated appendicitis at an outside institution. During his convalescence he underwent percutaneous drainage of a presumed postoperative abscess. A follow-up drain study demonstrated an enteric fistula. The drain was slowly removed from the abdomen over a period of 1 week. Three weeks following drain removal the patient reported recurrent nausea and abdominal pain. A CT scan demonstrated a 3.7-cm rim-enhancing air-fluid level with dependent contrast consistent with persistent enteric fistula and abscess. Exploratory laparoscopy was performed, at which time a Meckel diverticulum was identified and resected. This case highlights the diagnostic challenge and limitations of conventional radiology in complicated Meckel diverticulum. (orig.)

  20. A confirmed case of Rickettsia parkeri infection in a traveler from Uruguay.

    Science.gov (United States)

    Portillo, Aránzazu; García-García, Concepción; Sanz, M Mercedes; Santibáñez, Sonia; Venzal, José M; Oteo, José A

    2013-12-01

    The first confirmed case of Rickettsia parkeri infection in Uruguay is reported. To date, in South America, molecularly confirmed cases of human infection have been found in Argentina and probably, Brazil. Our patient returned to Spain after a 7-day trip to Colonia Suiza (Southwestern Uruguay). He presented fever (39°C), chills, and two eschars (tache noire-like) surrounded by an indurated, erythematous halo on the inner side of the left ankle besides a maculopapular rash on the legs. After treatment with doxycycline for 7 days, he fully recovered. R. parkeri infection was diagnosed by molecular-based detection of the microorganism in a swab specimen of the eschar. Diagnosis was supported by seroconversion between acute- and convalescent-phase sera specimens.

  1. Is LESS really more?

    Directory of Open Access Journals (Sweden)

    Joseph A Graversen

    2012-01-01

    Full Text Available In the last decade, laparoendoscopic single-site surgery (LESS has been touted to be the part of the ′evolution′ of minimally invasive surgery. The hope is that reduced access points will ultimately decrease pain, morbidity, convalescence, and improve cosmesis. However, what is unique about LESS is that while laparoscopic literature sought to demonstrate superiority of the technique over that of open surgery, the publications on LESS generally seem to seek to demonstrate equivalence with laparoscopy, with the major focus being on cosmesis. Unfortunately, even in that regard the objective cosmesis data is lacking. Furthermore, patients rate cosmesis the least important of all factors. LESS has also been associated with increased risk of complication, increased surgical cost, and longer operative times. In the current review, an objective assessment of the literature will be used for comparison between current standard laparoscopic techniques and LESS with the hopes of answering the question: is LESS really more?

  2. [Chickenpox, burns and grafts].

    Science.gov (United States)

    Rojas Zegers, J; Fidel Avendaño, L

    1979-01-01

    An outbreak of chickenpox that occurred at the Burns Repair Surgery Unit, Department of Children's Surgery, Hospital R. del Río, between June and November, 1975, is reported. 27 cases of burned children were studied, including analysis of correlations of the stages and outcome of the disease (varicela), the trauma (burns) and the graft (repair surgery). As a result, the authors emphasize the following findings: 1. Burns and their repair are not aggravating factors for varicella. In a small number of cases the exanthema looked more confluent in the graft surgical areas and in the first degree burns healing spontaneously. 2. Usually there was an uneventful outcome of graft repair surgery on a varicella patient, either during the incubation period, the acme or the convalescence. 3. The fact that the outmost intensity of secondary viremia of varicella occurs before the onset of exanthemia, that is, during the late incubation period, is confirmed.

  3. Seroepidemiology of Coxiella burnetii Infection and its Frequency as a Cause of Community-Acquired Pneumonia in Canada

    Directory of Open Access Journals (Sweden)

    Thomas J Marrie

    2002-01-01

    Full Text Available The present study tested acute and convalescent serum samples from 788 patients hospitalized for community-acquired pneumonia in seven Canadian provinces for antibodies to Coxiella burnetii. One hundred nine patients (13.8% had antibodies to this microorganism, and seven patients had acute Q fever. Serological evidence of infection with C burnetii was present in patients from all seven provinces. Three of the seven cases of acute Q fever were from Manitoba, suggesting that there may be unrecognized cases of Q fever in this province. In addition, a case of acute Q fever in Newfoundland, where there had previously been no reported cases, was noted, although subsequently, an outbreak of Q fever on goat farms has been reported.

  4. Using Latent Mixed Markov Models for the choice of the best pharmacological treatment.

    Science.gov (United States)

    Reuter, Martin; Hennig, Juergen; Netter, Petra; Buehner, Markus; Hueppe, Michael

    2004-05-15

    The choice of the best pharmacological treatment for an individual patient is crucial to optimize convalescence. Due to their effects on pharmacokinetics variables like gender and age are important factors when the pharmacological regimen is planned. By means of an example from anaesthesiology the usefulness of Latent Mixed Markov Models for choosing the optimal anaesthetic considering patient characteristics is demonstrated. Latent Mixed Markov models allow to predict and compare the quality of recovery from anaesthesia for different patient groups (defined by age and gender and treated with different anaesthetic regimens) in a multivariate non-parametric approach. On the basis of observed symptoms immediately after surgery and a few days later the probabilities for the respective dynamic latent status (like health or illness) and the probabilities for transition from one status to another are estimated depending on latent class membership (patient group).

  5. [The experience of a 'weak and sick childhood' far from home: the experience of the Maritime Asylum, Mar del Plata (1893-1920)].

    Science.gov (United States)

    Alvarez, Adriana

    2010-03-01

    The objective of this work is to analyze the daily life of 'tubercular children'" coming from the city of Buenos Aires. They were hospitalized in the Maritime Asylum located in the seaside city of Mar del Plata, 400 kilometers from the capital. In 1893, in the Charitable Society of the Federal Capital, the idea arose of founding a hospital and maritime asylum for children ill with osseous tuberculosis in general, weak and convalescing children and the treatment of patients with extra-pulmonary tuberculosis. This paper attempts to advance the understanding of two inter-related topics: on the one hand, the characteristics of the institutionalized meddling of the Charitable Society, and, on the other, the experience that these tubercular children had in this institution.

  6. 军事飞行人员积极情绪心理训练对疗养效果影响研究

    Institute of Scientific and Technical Information of China (English)

    李仑; 潘德福; 王煜蕙

    2015-01-01

    Objective To explore the effects of positive emotion training on military flight personnel in convales-cence.Methods 106 military flight personnel coming to our sanatoriumfor convalescence were randomly divided into the intervention group (positive emotion training group )and the control group (normal convalescence group ),with 53 military pilots in each group .The intervention group were given four times of positive emotion training in accordance with the emotion training programs,once a week and 50 minutes for once.Positive and N egative A ffect Scale(PANAS) and Life Satisfaction Scales were adopted to evaluate the convalescent effect in both groups before and after the conva-lescence.Results The positive and negative emotions and life satisfaction were of statistical significance in terms of the age and the plane model among the military flight personnel(P0 .05 ).Conclusion The emotion mental training programs oriented by positive psychology has a significant convalescent effect on emotion regu-lation for military flight personnel.%目的:探讨积极情绪心理训练应用于疗养军事飞行人员的实施效果。方法将106例来院疗养军事飞行人员随机分为干预组(积极情绪心理训练组)和对照组(正常疗养组),每组各53例,干预组按照情绪训练方案开展4次积极情绪心理训练,1次/周,50 min/次。两组均使用正负情绪情感量表和生活满意度量表前后施测对比评价疗养效果。结果入院军事飞行人员在年龄、机种变量上正负情绪和生活满意度均有统计学意义(P<0.05);干预组经过积极情绪心理训练后,积极情绪和生活满意度显著高于干预前,消极情绪显著低于干预前(P<0.05),对照组前后测差异未达到统计学意义(P>0.05)。结论以积极心理学为取向的情绪心理训练方案对军事飞行人员的情绪调节具有明显的疗养效果。

  7. Interpretation of microscopic agglutination test for leptospirosis diagnosis and seroprevalence

    Directory of Open Access Journals (Sweden)

    Chintana Chirathaworn

    2014-05-01

    Full Text Available Determination of antibody titer by microscopic agglutination test (MAT has been used as a tool for leptospirosis diagnosis. Four fold or greater rise in antibody titers between acute and convalescent sera suggests recent Leptospira infection. In addition, results obtained by MAT have been used to predict infecting serovars. However, cross reactivity among various Leptospira serovars have been reported when patient sera were tested with a battery of Leptospira serovars. This study demonstrates cross-reactivity among several Leptospira serovars when MAT was performed on leptospirosis sera. The data support a role of MAT as a tool for diagnosis. However, for information on infecting serovars, Leptospira isolation and molecular identification should be performed.

  8. Techniques applicable for purifying Chironex fleckeri (box-jellyfish) venom.

    Science.gov (United States)

    Othman, I; Burnett, J W

    1990-01-01

    A survey of several techniques to isolate a purified lethal factor from the tentacles of Chironex fleckeri was completed. Heterologous band patterns were obtained from specific eluates after gel filtration, ion exchange, immunoaffinity and hydrophobic chromatography. SDS-PAGE revealed a dense band at 24,000 mol. wt in many of these fractions. Isoelectric focusing of the crude venom resulted in considerable loss of activity but indicated significant purification in the fractions having a pI of 5.2-6.8. These fractions were also immunologically active against sera from a convalescing post-evenomation patient. The primary difficulties encountered in jellyfish venom purification are the lack of stability and the tendency of the active toxins to adhere to each other and to various support matrices.

  9. Is LESS really more?

    Science.gov (United States)

    Graversen, Joseph A; Lusch, Achim; Landman, Jaime

    2012-01-01

    In the last decade, laparoendoscopic single-site surgery (LESS) has been touted to be the part of the 'evolution' of minimally invasive surgery. The hope is that reduced access points will ultimately decrease pain, morbidity, convalescence, and improve cosmesis. However, what is unique about LESS is that while laparoscopic literature sought to demonstrate superiority of the technique over that of open surgery, the publications on LESS generally seem to seek to demonstrate equivalence with laparoscopy, with the major focus being on cosmesis. Unfortunately, even in that regard the objective cosmesis data is lacking. Furthermore, patients rate cosmesis the least important of all factors. LESS has also been associated with increased risk of complication, increased surgical cost, and longer operative times. In the current review, an objective assessment of the literature will be used for comparison between current standard laparoscopic techniques and LESS with the hopes of answering the question: is LESS really more?

  10. [Laser hair removal for urethral hair after hypospadias repair].

    Science.gov (United States)

    Kaneko, Tomoyuki; Nishimatsu, Hiroaki; Ogushi, Tetsuo; Sugimoto, Masayuki; Asakage, Yasuyuki; Kitamura, Tadaichi

    2008-01-01

    A 56-year-old male was admitted for induration of ventral side of the penile shaft. Computed tomography showed a large urethral calculus in the distal urethra. About 50 years previously, he had undergone multi-staged urethroplasty for hypospadias. He had also suffered from recurrent urethral calculi managed by urethrolithotomy 5 and 2 years before the admission. Urethrolithotomy revealed hair-bearing urethral calculus. Instillation of depilating agent containing thioglycolate into the neourethra for preventing hair regrowth was ineffective. Transurethral laser hair removal of neourethra was subsequently performed. All the neourethral follicles were ablated with GaAlAs diode laser (wave length 810 nm; at a power of 15W for 2 seconds) through a side-firing laser fiber. Another three operations were performed for a few regrown hairs at a power of 20-30W. Convalescence was uneventful. The patient is free of hair regrowth except for a hair at five months of follow-up.

  11. Production of High Potency Anti-Teschen Disease Serum From Rabbits and Guinea Pigs.

    Science.gov (United States)

    Dardiri, A H; Delay, P D

    1964-01-01

    An antiserum of high antibody content against Teschen disease Konratice virus was obtained by inoculating rabbits and guinea pigs with an antigen composed of aluminum gel and virus propagated in swine kidney cell cultures. The rabbit and guinea pig serums neutralized 1,500 TCID(50) of virus at dilutions of 1:5,120 and 1:2,048, respectively. The antibody level in the rabbit serum was tenfold greater than that in convalescent swine serum. Rabbit serum neutralized 2.8 x 10(6) plaque-forming units of the Konratice virus. At a dilution of 1:5,120, this serum neutralized 1,500 TCID(50) of the Reporyje virus. The methods used to prepare and assay the serum are described.

  12. Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak.

    Science.gov (United States)

    Bornholdt, Zachary A; Turner, Hannah L; Murin, Charles D; Li, Wen; Sok, Devin; Souders, Colby A; Piper, Ashley E; Goff, Arthur; Shamblin, Joshua D; Wollen, Suzanne E; Sprague, Thomas R; Fusco, Marnie L; Pommert, Kathleen B J; Cavacini, Lisa A; Smith, Heidi L; Klempner, Mark; Reimann, Keith A; Krauland, Eric; Gerngross, Tillman U; Wittrup, Karl D; Saphire, Erica Ollmann; Burton, Dennis R; Glass, Pamela J; Ward, Andrew B; Walker, Laura M

    2016-03-01

    Antibodies targeting the Ebola virus surface glycoprotein (EBOV GP) are implicated in protection against lethal disease, but the characteristics of the human antibody response to EBOV GP remain poorly understood. We isolated and characterized 349 GP-specific monoclonal antibodies (mAbs) from the peripheral B cells of a convalescent donor who survived the 2014 EBOV Zaire outbreak. Remarkably, 77% of the mAbs neutralize live EBOV, and several mAbs exhibit unprecedented potency. Structures of selected mAbs in complex with GP reveal a site of vulnerability located in the GP stalk region proximal to the viral membrane. Neutralizing antibodies targeting this site show potent therapeutic efficacy against lethal EBOV challenge in mice. The results provide a framework for the design of new EBOV vaccine candidates and immunotherapies.

  13. Extracorporeal shockwave therapy in the treatment of stress fracture of tibia. Report of a case

    Directory of Open Access Journals (Sweden)

    Santiago Gómez García

    2015-02-01

    Full Text Available Pain in the lower limbs induced by exercise is common in military cadets due to long periods of jogging as part of his training and is related to overtraining. Stress fractures of tíbia are common among the disorders affecting them but most often are not diagnosed. The period of convalescence is prolonged sometimes leading to abandon their atividades. A case of an affected cadet to which treated by extracorporeal shockwave therapy showing reduced pain at rest and increased time trotting achieved without painful. Because of the importance that medical staff primarily Orthopaedic Surgeons and Sports Physicians and other health professionals involved in the care know about the existence of pathology as this new therapeutic alternative is that the case is presented.

  14. Experimental rhinovirus infection in human volunteers exposed to ozone

    Energy Technology Data Exchange (ETDEWEB)

    Henderson, F.W.; Dubovi, E.J.; Harder, S.; Seal, E. Jr.; Graham, D.

    1988-05-01

    We studied 24 young adult male volunteers experimentally inoculated with type 39 rhinovirus to determine whether the course of viral infection was modified by exposure to moderate levels of ozone (0.3 ppm for 6 h per day) over the 5 days after virus inoculation. No differences in rhinovirus titers in nasal secretions, recruitment of neutrophils into nasal secretions, levels of interferon in nasal lavage fluid, in vitro lymphocyte proliferative responses to rhinovirus antigen, or levels of convalescent serum neutralizing antibody to type 39 rhinovirus were demonstrated in relation to ozone exposure. The level and pattern of ozone exposure used in this experiment had no demonstrable adverse effects on the immune responses necessary to limit and terminate rhinovirus infection of the upper respiratory tract.

  15. CLONING SEGMENT SPIKE PROTEIN GENE OF SARS-COV AND ITS EXPRESSION IN ESCHERICHIA COLI

    Institute of Scientific and Technical Information of China (English)

    刘中华; 许文波; 毛乃颖; 张燕; 朱贞; 崔爱利; 杨建国; 胡海涛

    2004-01-01

    Objective Expressing and purifying the segment of SARS-CoV spike protein in E.Coli. Methods The target gene was obtained by RT-PCR. The PCR product was cloned into pEGM- T Easy Vector, sequencing and double restriction digestion ( BamHⅠ,PstⅠ) were performed. The target gene was subcloned into PQE30 expression vector. The gene was expressed in the E.coli strain M15 cells induced by IPTG. The protein was purified with a nickel HiTrap chelating metal affinity column. Results The recombinant expression plasmid was successfully constructed and the protein was well expressed in E. coli strain M15 cells. The ideal pure protein was obtained by purification. Western blotting analysis suggested the protein could act with the convalescent sera of lab confirmed SARS patients. Conclusion The segment of SARS-CoV spike protein was well expressed and purified, and can be applied in diagnosis and immunological research of SARS.

  16. 电子疗案在应用中的问题及解决的方案%Problems and Solutions of Electronic Medical Record in the Application Program

    Institute of Scientific and Technical Information of China (English)

    王琇

    2013-01-01

    the concept of electronic medical record, the characteristics, problems and solutions are discussed. Electronic medical case related to diagnosis and treatment, the convalescent nursing information such as data entry, analysis, management and filing. To improve the nursing home management level, promote the sustainable development of the sanitarium.%对电子疗案的概念、特点、存在的问题及解决的方案进行了探讨。电子疗案对疗养员的相关诊疗、护理措施等信息资料进行录入、分析、管理和归档。提高了疗养院的现代化管理水平,促进了疗养院可持续发展。

  17. Strong IgG antibody responses to Borrelia burgdorferi glycolipids in patients with Lyme arthritis, a late manifestation of the infection.

    Science.gov (United States)

    Jones, Kathryn L; Seward, Robert J; Ben-Menachem, Gil; Glickstein, Lisa J; Costello, Catherine E; Steere, Allen C

    2009-07-01

    In this study, the membrane lipids of B. burgdorferi were separated into 16 fractions; the components in each fraction were identified, and the immunogenicity of each fraction was determined by ELISA using sera from Lyme disease patients. Only the 2 glycolipids, acylated cholesteryl galactoside (ACG, BbGL-I) and monogalactosyl diacylglycerol (MgalD, BbGL-II), were immunogenic. Early in the infection, 24 of 84 patients (29%) who were convalescent from erythema migrans and 19 of the 35 patients (54%) with neuroborreliosis had weak IgG responses to purified MgalD, and a smaller percentage of patients had early responses to synthetic ACG. However, almost all of 75 patients with Lyme arthritis, a late disease manifestation, had strong IgG reactivity with both glycolipids. Thus, almost all patients with Lyme arthritis have strong IgG antibody responses to B. burgdorferi glycolipid antigens.

  18. ISOLATION AND CHARACTERIZATION OF AVIAN INFLUENZA (H9N2 VIRUS FROM AN OUTBREAK AT POULTRY FARMS IN KARACHI

    Directory of Open Access Journals (Sweden)

    Mohammad A. Muneer, Ali Mohammad Bahram, Zahid Munir, I. Hussain, K. Muhammad, Masood Rabbani, S. Akhtar, M. Aleem, Bakht Sultan1, Munir A. Tariq and K. Naeem2

    2001-02-01

    Full Text Available Avian influenza virus (AIV type H9N2 was isolated from poultry flocks which were suffering from acute respiratory illness in Karachi area. High haemagglutination inhibition (HI titers against AIV virus type H9N2, ranging from 6.38 to 7.81, in the convalescent sera of birds were demonstrated. The infected flocks had mortality between 30 to 80 per cent. Those flocks also had considerable of HI titres against infectious bronchitis (IB virus stains D274 and D1466, against which they were never vaccinated. It is believed that AI H9N2 virus in collaboration with IBV and some unidentified bacterial species caused high mortality in the infected flocks

  19. [Counterimmunoelectrophoresis in the diagnosis of Bordetella pertussis].

    Science.gov (United States)

    Grinstein, S; López, E L; Muchinik, G; Duhart, E; Bonesana, N

    1979-01-01

    The serum of 50 patients between 25 days to 15 years old who were admitted with the diagnosis of pertussis syndrome were investigated for precipitating antibodies by means of discontinuous counterimmunolectrophoresis (CIED). The presence of antibodies for Bordetella pertussis antigen was shown in 28 cases. All samples were taken within the first 24/48 hours of admission and at convalescence. The technique is easy to carry out, quick and of low cost. This test offers an adequate and fast means to differentiate a Bordetella pertussis syndrome form others produced by different viral etiologies. Besides, this serologic technique shows earlier results and of lower cost than classic techniques such as agglutination and complement fixation which are more difficult to apply.

  20. Modern approaches to parodontitis therapy in HIV patients

    Directory of Open Access Journals (Sweden)

    Shuldyakov A.A.

    2011-12-01

    Full Text Available The research goal is to determine the clinical pathogenetic efficacy of Cycloferon liniment in the combined therapy of periodontitis in patients with subclinical stage of HIV-infection. Medical examination and treatment of 40 patients has been carried out. It is established that use of liniment Cycloferon in the combined treatment of patients with subclinical stage of HIV-infection allows to accelerate process of normalization of lipid peroxidation parameters and antioxidant potential of blood and to decrease infection (herpes symplex virus I, Candida albicans, staphylococcus aureus in par-odontal recess and evidence of local inflammation. The considered method of therapy has been proved to improve convalescence period and to decrease frequency of parodontitis recurrences

  1. A cluster of primary pneumonic plague transmitted in a truck cab in a new enzootic focus in China.

    Science.gov (United States)

    Luo, Huiming; Dong, Xingqi; Li, Furong; Xie, Xu; Song, Zhizhong; Shao, Zhujun; Li, Zhongjie; Tong, Zhaohui; Wang, Guangfa; Zhang, Hongtao; Yang, Tielong; He, Gao; He, Zeyuan; Fontaine, Robert E; Zeng, Guang

    2013-05-01

    We investigated a cluster of five cases of severe pneumonia from one village in Yunnan Province, China. We searched for severe pneumonia in the village and hospitals. We interviewed patients and family members about exposures. We tested acute and convalescent sera for antigen and antibody of severe acute respiratory syndrome, avian influenza, and plague. The only common exposure of the five patients was riding together in the enclosed cab of a truck for 1.5 hours while taking the first patient to the hospital. Seroconversion to plague F1 antigen confirmed plague in three survivors. Unfamiliarity of clinicians with plague and lack of sputum examination, blood culture, or postmortem examination delayed the diagnosis. No plague cases occurred among family and village contacts and health care workers. High infectivity in this cluster was limited to a crowded, poorly ventilated truck.

  2. Evolution of endoscopic extraperitoneal radical prostatectomy (EERPE): technique and outcome

    Institute of Scientific and Technical Information of China (English)

    Jens-Uwe Stolzenburg; Odysseas Andrikopoulos; Panagiotis Kallidonis; Iason Kyriazis; Minh Do; Evangelos Liatsikos

    2012-01-01

    Endoscopic extraperitoneal radical prostatectomy (EERPE) is a well-established and standardized technique for treating patients with localized prostate cancer.Nevertheless,the procedure is continuously being refined with the expansion of anatomical knowledge.The development of a nerve-sparing approach and improvements in currently used equipment are expected to yield better results in cosmesis and convalescence without sacrificing the procedure's established benefits in terms of potency,continence and oncological management.In this study,the technique and its evolution are presented in detail,along with an analysis of its clinical efficacy.We also consult the literature to compare EERPE to transperitoneal laparoscopic radical prostatectomy,and we also discuss new technical advancements regarding the use of robotic assistance during EERPE.

  3. Increased physiological dead space/tidal volume ratio during exercise in burned children.

    Science.gov (United States)

    Mlcak, R P; Desai, M H; Robinson, E; McCauley, R L; Richardson, J; Herndon, D N

    1995-08-01

    Exercise testing enables the simultaneous evaluation of the cardiovascular and respiratory systems' ability to perform gas exchange. The physiological responses to exercise have not been previously reported in the postburn child. This investigation was designed to evaluate residual cardiopulmonary impairment in patients convalescing from severe burns. Spirometry, lung volumes and exercise stress testing were completed on 40 children with a mean time postburn injury of 2.6 +/- 1.9 years and mean burn size of 44 +/- 22 per cent TBSA. Respiratory variables studied during exercise included expired volume, tidal volume and respiratory rate, and physiological dead space/tidal volume (VD/VT) ratios. Stress testing revealed an increased VD/VT ratio consistent with uneven ventilation-perfusion relationships. The data indicate that patients who survive thermal injury may not regain normal cardiopulmonary homeostasis.

  4. Imported zika virus infection from the cook islands into australia, 2014.

    Science.gov (United States)

    Pyke, Alyssa T; Daly, Michelle T; Cameron, Jane N; Moore, Peter R; Taylor, Carmel T; Hewitson, Glen R; Humphreys, Jan L; Gair, Richard

    2014-06-02

    A female resident of Townsville, Queensland, Australia has been diagnosed with Zika virus infection following a recent trip to the Cook Islands. An initial serum sample collected in March, 2014 was positive by two separate Zika virus TaqMan real-time RT-PCRs and a pan-Flavivirus RT-PCR. Nucleotide sequencing and phylogenetics of the complete Cook Islands Zika virus envelope gene revealed 99.1% homology with a previous Cambodia 2010 sequence within the Asian lineage. In addition, IgG and IgM antibody seroconversions were detected between paired acute and convalescent phase sera using recombinant Zika virus serology assays. This is the first known imported case of Zika virus infection into northern Queensland where the potential mosquito vector Aedes aegypti is present and only the second such reported case diagnosed within Australia.

  5. Culture-independent microbiological analysis of foley urinary catheter biofilms.

    Directory of Open Access Journals (Sweden)

    Daniel N Frank

    Full Text Available BACKGROUND: Prevention of catheter-associated urinary tract infection (CAUTI, a leading cause of nosocomial disease, is complicated by the propensity of bacteria to form biofilms on indwelling medical devices [1,2,3,4,5]. METHODOLOGY/PRINCIPAL FINDINGS: To better understand the microbial diversity of these communities, we report the results of a culture-independent bacterial survey of Foley urinary catheters obtained from patients following total prostatectomy. Two patient subsets were analyzed, based on treatment or no treatment with systemic fluoroquinolone antibiotics during convalescence. Results indicate the presence of diverse polymicrobial assemblages that were most commonly observed in patients who did not receive systemic antibiotics. The communities typically contained both Gram-positive and Gram-negative microorganisms that included multiple potential pathogens. CONCLUSION/SIGNIFICANCE: Prevention and treatment of CAUTI must take into consideration the possible polymicrobial nature of any particular infection.

  6. Music for the injured soldier: a contribution of American women's military bands during World War II.

    Science.gov (United States)

    Sullivan, Jill M

    2007-01-01

    This study is an investigation of the contributions of women's military bands in the United States to the reconditioning of the injured American troops during World War II. Primary and secondary sources revealed that these bands welcomed home hospital ships, performed for convalescing soldiers in hospitals, and provided music for hospital dances. While each of the bands investigated served in similar capacities, only one, the 403rd Women's Army Corps (WAC) Band, was stationed at a hospital. While entertainment by women's bands was an important part of the Army Reconditioning Program for the injured, the study also revealed a working partnership that developed between these musicians and the medical community. Sixty years after the war, band members believe their performances in hospitals were the most important contribution of their service. Some historians have concluded that music used in military hospitals during the war was the impetus for the music therapy profession.

  7. Children with Haemophilus influenzae type b (Hib) vaccine failure have long-term bactericidal antibodies against virulent Hib strains with multiple capsular loci.

    Science.gov (United States)

    Townsend-Payne, Kelly; Ladhani, Shamez N; Findlow, Helen; Slack, Mary; Borrow, Ray

    2016-07-25

    Children who develop invasive Haemophilus influenzae serotype b (Hib) disease after immunisation with a highly-effective conjugate vaccine are more likely to have been infected with Hib strains possessing multiple copies of the capsulation locus. Using a recently-validated serum bactericidal antibody (SBA) assay, we tested convalescent sera from 127 Hib vaccine failure cases against clinical Hib strains expressing 1-5 copies of the capsulation locus. SBA titres correlated weakly with anti-capsular IgG antibody concentrations and there was no association between SBA geometric mean titres and number of capsulation locus copies. After infection, children with Hib vaccine failure were equally protected against Hib strains with 1-5 copies of the capsulation locus.

  8. Robotic Radical Cystectomy: Where are We Today, Where will We be Tomorrow?

    Directory of Open Access Journals (Sweden)

    Kyle A. Richards

    2010-01-01

    Full Text Available While open radical cystectomy remains the gold-standard treatment for muscle-invasive bladder cancer and high-risk non–muscle invasive disease, robotic assisted radical cystectomy (RARC has been gaining popularity over the past decade. The robotic approach has the potential advantages of less intraoperative blood loss, shorter hospital stay, less post-operative narcotic requirement, quicker return of bowel function, and earlier convalescence with an acceptable surgical learning curve for surgeons adept at robotic radical prostatectomy. While short to intermediate term oncologic results from several small RARC series are promising, bladder cancer remains a potentially lethal malignancy necessitating long-term follow-up. This article aims to review the currently published literature, important technical aspects of the operation, oncologic and functional outcomes, and the future direction of RARC.

  9. Physiotherapy Exercise After Fast-Track Total Hip and Knee Arthroplasty: Time for Reconsideration?

    DEFF Research Database (Denmark)

    Bandholm, Thomas; Kehlet, Henrik

    2012-01-01

    Bandholm T, Kehlet H. Physiotherapy exercise after fast-track total hip and knee arthroplasty: time for reconsideration? Major surgery, including total hip arthroplasty (THA) and total knee arthroplasty (TKA), is followed by a convalescence period, during which the loss of muscle strength......-track methodology or enhanced recovery programs. It is the nature of this methodology to systematically and scientifically optimize all perioperative care components, with the overall goal of enhancing recovery. This is also the case for the care component "physiotherapy exercise" after THA and TKA. The 2 latest...... meta-analyses on the effectiveness of physiotherapy exercise after THA and TKA generally conclude that physiotherapy exercise after THA and TKA either does not work or is not very effective. The reason for this may be that the "pill" of physiotherapy exercise typically offered after THA and TKA does...

  10. Intra-articular morphine in horses

    DEFF Research Database (Denmark)

    Lindegaard, Casper

    Regardless of species, optimal pain management of animals subjected to various painful procedures is of outmost importance for several reasons, including animal welfare considerations, improved convalescence and improved final outcome. One way of improving pain management in horses is through...... and laboratory animals. Recent discovery of opioid receptors in the synovial membrane of horses has made it reasonable to expect IA morphine to be analgesic in horses too. Treatment with IA morphine after arthroscopic surgery, or for other painful joint diseases, might therefore be an important contribution...... to a multimodal analgesia protocol. Despite that no research has investigated this issue in horses so far, IA injection of morphine after arthroscopic surgery has become common practice in several veterinary university teaching hospitals in Europe and USA. The aims of this thesis were to investigate the analgesic...

  11. Gerstmann's syndrome following an acute herpes simplex encephalitis.

    Science.gov (United States)

    Ilchevsky, S; Boev, I; Kazakova, T

    1998-01-01

    The authors present a rare clinical case of a woman who developed Gerstmann's syndrome following an acute Herpes simplex viral encephalitis. Clinical observation and laboratory evaluation were performed during the acute phase of the disease. After that the follow-up continued for one-year period. The localization of the pathologic process was determined by computerized tomography, conducted periodically. The characteristics of the clinical picture are interpreted in the context of the contemporary concepts of the topical diagnosis of Gerstmann's syndrome. The possibility of a sudden onset of acute Herpes simplex viral encephalitis without a preceding febrile-intoxication syndrome is worth noting. Conclusions are drawn stressing the need of an early etiologic treatment and the importance of the rehabilitation activities during the convalescence period.

  12. Immunity to VHS virus in rainbow trout

    DEFF Research Database (Denmark)

    Lorenzen, Niels; Olesen, Niels Jørgen; Koch, C.

    1999-01-01

    Viral hemorrhagic septicemia virus (VHSV) is the rhabdovirus that causes most disease problems in farmed rainbow trout in Europe. Survivors of infection are usually immune to reinfection but as with other fish viruses, development of a modern recombinant vaccine has been complicated by the limited...... knowledge of the immune mechanisms and antigens involved in induction of immunity. Neutralizing and protective monoclonal antibodies recognize the envelope glycoprotein (G protein) which is the only viral protein known to be present on the surface of the virus particle. Immunoblotting analyses...... with monoclonal antibodies as well as with sera from immunized trout have indicated that protein conformation plays an important role in neutralization epitopes. The virus neutralizing activity often found in sera from convalescent trout is highly dependent on a poorly defined complementing activity in normal...

  13. Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques

    Directory of Open Access Journals (Sweden)

    Om Parkash

    2015-10-01

    Full Text Available Dengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention and treatment of dengue disease. Moreover, clinical features of dengue are indistinguishable from other infectious diseases such as malaria, chikungunya, rickettsia and leptospira. Therefore, prompt and accurate laboratory diagnostic test is urgently required for disease confirmation and patient triage. The traditional diagnostic techniques for the dengue virus are viral detection in cell culture, serological testing, and RNA amplification using reverse transcriptase PCR. This paper discusses the conventional laboratory methods used for the diagnosis of dengue during the acute and convalescent phase and highlights the advantages and limitations of these routine laboratory tests. Subsequently, the biosensor based assays developed using various transducers for the detection of dengue are also reviewed.

  14. [Comparative assessment of conservative treatment of chronic prostatitis with application of complex unit andro-gin].

    Science.gov (United States)

    Golubchikova, V A; Alekseev, M Ia; Sitnikov, N V; Kochetov, A G; Podgornyĭ, V F

    2000-01-01

    The Andro-Gin unit used in combined treatment of 78 patients with chronic prostatitis (CP) and 16 women with chronic interstitial cystitis with inflammation in the internal genitalia (56.5% of the patients had sexual and spermatogenesis disorders) employed effects of simultaneous exposure to local magnetic field, electrostimulation of the small pelvis organs and prostate with introduction of contact rectal electrode, neurostimulation of pathogenic zones, low-intensity laser and light-diode radiator and color impulse therapy. Control group consisted of 85 patients with CP who received conventional physiotherapy. The comparative analysis has shown high efficiency of the unit Andro-Gin in the treatment of CP and its complications. Laboratory and clinical convalescence and persistent remission was registered in 98% of the patients of the study group. Combined treatment using Andro-Gin is twice more effective than other treatments.

  15. Immune response in diarrheal patients and asymptomatic carrier with CS6-producing enterotoxigenic Escherichia coli infection.

    Science.gov (United States)

    Puiprom, Orapim; Chantaroj, Siriporn; Matsuda, Shigeaki; Sawanpanyalert, Pathom; Honda, Takeshi; Iida, Tetsuya; Taniguchi, Tooru

    2012-11-01

    Enterotoxigenic Escherichia coli (ETEC) is one of the major causes of diarrhea in children and travelers in developing countries. ETEC colonization factors (CFs) are virulence determinants considered as protective antigens and major targets for vaccine development against ETEC infections. One of the most prevalent CFs, coli surface antigen 6 (CS6), a non-fimbrial polymeric protein consisting of two major subunits, CssA and CssB, is produced by approximately 25-35% of ETEC worldwide. We could isolate only CS6-producing ETEC strains from two diarrheal patients and one asymptomatic carrier, but we could not detect CssA- or CssB-specific antibodies in the feces and blood of two patients convalescing from natural ETEC infection and of an asymptomatic carrier using western blotting. Therefore, in order to protect against infection with CS6-producing ETEC, protective levels of CS6 immunity should be incorporated in any future vaccines against ETEC.

  16. [Scarlet fever epidemic during year 1929 in Chile].

    Science.gov (United States)

    Laval R, Enrique

    2009-04-01

    Scarlet fever is endemic in Chile, with relatively low morbidity and periodic exacerbations every 4 or 5 years, which can become epidemics. From 1921 to 1927, the number of patients hospitalized in the country fluctuated from 15 to 65 per year, until an epidemic involving nearly 3.000 patients started at the end of 1928 and continued during all 1929. 978 patients died, 537 (52.5%) were from Santiago. Public Health authorities confronted this emergency with prevention, prophylaxis, isolation and treatment measures and 558 beds were disposed for patient hospitalization. Vaccination trials were undertaken and specific treatment with antitoxins was used in patients with toxic clinical cases, having satisfactory results. Convalescent patients were controlled in order to stop the spread of the infection. After approximately 3 years, this disease returned to its regular endemicity.

  17. [Clinical and immunological features of acute hepatitis B in patients with concomitant chronic toxic liver damage].

    Science.gov (United States)

    Furyk, E; Ryabokon, E

    2013-02-01

    The article presents information obtained during the survey in 64 patients with acute hepatitis B. We show that acute hepatitis B in patients with concomitant chronic toxic liver characterized by a marked imbalance of cytokine status due to a lower level of interleukin-2 and a higher content of interleukin-8, the highest levels of nitrite content, spontaneous oxidative modifications of blood proteins and the lowest content of L -arginine in the blood serum in the dynamics of disease compared with patients without this concomitant factor. In the period of convalescence these changes in patients with acute hepatitis B with concomitant chronic toxic liver characterized combined with higher cytolysis of liver cells, often circulating in the blood of HBsAg seroconversion and less frequently with the advent of anti-HBeAg.

  18. Comparison of the Performance of the TPTest, Tubex, Typhidot and Widal Immunodiagnostic Assays and Blood Cultures in Detecting Patients with Typhoid Fever in Bangladesh, Including Using a Bayesian Latent Class Modeling Approach.

    Science.gov (United States)

    Islam, Kamrul; Sayeed, Md Abu; Hossen, Emran; Khanam, Farhana; Charles, Richelle C; Andrews, Jason; Ryan, Edward T; Qadri, Firdausi

    2016-04-01

    There is an urgent need for an improved diagnostic assay for typhoid fever. In this current study, we compared the recently developed TPTest (Typhoid and Paratyphoid Test) with the Widal test, blood culture, and two commonly used commercially available kits, Tubex and Typhidot. For analysis, we categorized 92 Bangladeshi patients with suspected enteric fever into four groups: S. Typhi bacteremic patients (n = 28); patients with a fourfold change in Widal test from day 0 to convalescent period (n = 7); patients with Widal titer ≥1:320 (n = 13) at either acute or convalescent stage of disease; and patients suspected with enteric fever, but with a negative blood culture and Widal titer (n = 44). We also tested healthy endemic zone controls (n = 20) and Bangladeshi patients with other febrile illnesses (n = 15). Sample size was based on convenience to facilitate preliminary analysis. Of 28 S. Typhi bacteremic patients, 28 (100%), 21 (75%) and 18 (64%) patients were positive by TPTest, Tubex and Typhidot, respectively. In healthy endemic zone controls, the TPTest method was negative in all, whereas Tubex and Typhidot were positive in 3 (15%) and 5 (25%), respectively. We then estimated sensitivity and specificity of all diagnostic tests using Bayesian latent class modeling. The sensitivity of TPTest, Tubex and Typhidot were estimated at 96.0% (95% CI: 87.1%-99.8%), 60.2% (95% CI: 49.3%-71.2%), and 59.6% (95% CI: 50.1%-69.3%), respectively. Specificity was estimated at 96.6% (90.7%-99.2%) for TPTest, 89.9% (79.6%-96.8%) for Tubex, and 80.0% (67.7%-89.7%) for Typhidot. These results suggest that the TPTest is highly sensitive and specific in diagnosing individuals with typhoid fever in a typhoid endemic setting, outperforming currently available and commonly used alternatives.

  19. Herd immunity to GII.4 noroviruses is supported by outbreak patient sera.

    Science.gov (United States)

    Cannon, Jennifer L; Lindesmith, Lisa C; Donaldson, Eric F; Saxe, Lauryn; Baric, Ralph S; Vinjé, Jan

    2009-06-01

    Noroviruses (NoVs) of genogroup II, cluster 4 (GII.4), are the most common cause of outbreaks of acute gastroenteritis worldwide. During the past 13 years, GII.4 NoVs caused four seasons of widespread activity globally, each associated with the emergence of a new strain. In this report, we characterized the most recent epidemic strain, GII.4-2006 Minerva, by comparing virus-like particle (VLP) antigenic relationships and histo-blood group antigen (HBGA) binding profiles with strains isolated earlier. We also investigated the seroprevalence and specificity of GII.4 antibody in the years prior to, during, and following the GII.4 pandemic of 1995 and 1996 using a large collection of acute- and convalescent-phase serum pairs (n = 298) collected from 34 outbreaks. In a surrogate neutralization assay, we measured the blockade of HBGA binding using a panel of GII.4 VLPs representing strains isolated in 1987, 1997, 2002, and 2006 and a GII.3 VLP representing a strain isolated in the mid-1990s. Serum titers required for 50% HBGA blockade were compared between populations. In general, blockade of GII.4 VLP-HBGA binding was greater with convalescent-phase outbreak sera collected near the time of origin of the VLP strain. Heterotypic genotypes did not contribute to herd immunity against GII.4 NoVs based on their inability to block GII.4 VLP binding to HBGA. However, previous exposure to GII.4 NoV followed by infection by GII.3 NoV appeared to evoke an immune response to GII.4 NoV. These results support the hypothesis that herd immunity is a driving force for GII.4 evolution in the U.S. population. The data also suggest that complex patterns of cross-protection may exist across NoV genotypes in humans.

  20. Herpes simlex encephalitis. A neuropsychological and neuroradiological study

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    Koga, Masaya [Kurume Univ., Fukuoka (Japan). School of Medicine

    1994-10-01

    Herpes simplex virus (HSV) is the most common causative pathogen of nonepidemic encephalitis in Japan. The mortality rate is approximately 30% and surviving patients often have severe sequelae such as amnestic syndrome. Between 1979 and 1992, 13 patients with herpes simplex encephalitis (HSE) were admitted to our Department. Diagnosis was based on the neurological symptoms and signs, the findings on EEG, CT and MRI, and the results of serological examination including ELISA for HSV type 1. Neuropsychological tests were performed over a period of approximatly 2 years in seven of 13 patients. The results of the tests disclosed memory impairments as follows. Namely, immediate recall was well preserved, but disturbance of recent memory and retrograde amnesia over 20 years were observed. The neuropsychological features more closely resembled those of amnestic syndrome than those of Korsakoff syndrome. The amnesia in HSE patients was characterized in the finding that it was prone to recover more gradually as compared with that following head trauma or surgical brain resection. CT scan was performed in 13 patients during the acute and convalescent stages. The low density areas were found in the temporal lobes in 60% of the patients. MRI were obtained during the convalescent stage in ten of 13 patients. In seven of the ten patients, abnormal signals were distributed uni- or bilaterally to the temporal lobe, insular and limbic system such as the hippocampus and amygdala. In the remaining three patients, the abnormal signals were localized in both hippocampi and amygdalae in one patient and only in the brain stem in another. In the last case, the MRI was normal. The findings that signal intensity on MRI showing the lesion in the temporal lobe spread continuously to the opposite limbic area or occipital lobe strongly suggested the spreading of HSV through neural fiber connections in HSE. (author).

  1. PLATELET COUNT IN SEROPOSITIVE AND SERONEGATIVE DENGUE CASES IN RAICHUR DISTRICT

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    Inder Raj Itagi

    2014-12-01

    Full Text Available Dengue Fever is caused by Dengue Viruses (4 Serotypes by the bites of aedes aegypti mosquito. Laboratory findings in dengue cases show leucopenia and thrombocytopenia which is mild in nature. In this study we have made an attempt to compare platelet count in seropositive and seronegative dengue cases in and around Raichur District. AIMS AND OBJECTIVES: To compare the platelet count in seropositive and seronegative dengue fever patients. MATERIALS AND METHODS USED: Automated cell counter (SYSMEX-5PART. Specimen: Blood or serum in a red top tube. Acute and convalescent specimens do not need be sent together. Collection: KHEL Serology kit with the yellow (red top blood tubes or any other red topped, clot separator blood tubes. Volume: 2 cc (ml. of centrifuged serum or plasma. Storage: On ice or in refrigerator (not in a freezer until it is delivered to CDC Dengue Branch. Any specimens stored greater than a month prior to arrival at CDC will not be tested. Timing of collection for serology: Acute- obtained up to 5 days after onset of symptoms; convalescent- 6 or more days after the onset of symptoms. Test results are normally available 3 days (PCR to 1 week (serology after specimen receipt. During periods of a severe dengue epidemic it may be necessary to prioritize testing based on the severity of disease. Any severe case that is hospitalized should be indicated on the form. Type of Study: PROSPECTIVE (COHORT STUDY Duration of Study: 6 months (Dec-2011 to May 2012. Study Site: RAICHUR DISTRICT IN KARNATAKA.

  2. Passive antibody therapy of Lassa fever in cynomolgus monkeys: importance of neutralizing antibody and Lassa virus strain.

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    Jahrling, P B; Peters, C J

    1984-05-01

    Lassa virus-infected cynomolgus monkeys were passively immunized with immune plasma of primate or human origin to gain insight into criteria for plasma selection and administration to human Lassa fever patients. Protective efficacy was correlated with neutralizing antibody concentrations, expressed as a log10 neutralization index (LNI). Convalescent Lassa-immune monkey plasma was titrated for protective efficacy in monkeys by intravenous inoculation with dilutions of plasma on the day of subcutaneous Lassa virus inoculation (day 0) and again on days 3 and 6. Monkeys that received undiluted plasma (LNI = 4.1) (1 ml/kg per treatment) survived a lethal viral dose, whereas those given a 1:3 dilution (LNI = 2.6) of this same plasma (1 ml/kg per treatment) died. Protection was restored when the volume of the 1:3 plasma dilution was increased to 3 ml/kg per treatment. Plasma diluted 1:9 or more (LNI = 1.5 or less) delayed onset and suppressed the magnitude of viremia but failed to confer protection at 3 ml/kg per treatment. Immunological enhancement, defined as increased viremia or accelerated death, did not occur following inadequate treatment. Human convalescent plasma also protected recipient monkeys; reductions in mortality and viremia were accurately predicted by the LNI of the plasma. Plasma of Liberian origin neutralized a Liberian Lassa strain more effectively than a Sierra Leone strain in vitro (LNI = 2.8 and 1.6, respectively) and protected monkeys more effectively against the Liberian strain. Geographic origin is thus a factor in the selection of optimal plasma for treatment of human Lassa fever, since geographically matched plasma is more likely to contain adequate LNI titers against homologous Lassa virus strains.(ABSTRACT TRUNCATED AT 250 WORDS)

  3. Changes in Antibody Levels during and following an Episode of Acute Adenolymphangitis (ADL among Lymphedema Patients in Leogane, Haiti.

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    Katherine E Mues

    Full Text Available Episodes of acute adenolymphangitis (ADL are often the first clinical sign of lymphatic filariasis (LF. They are often accompanied by swelling of the affected limb, inflammation, fever, and general malaise and lead to the progression of lymphedema. Although ADL episodes have been studied for a century or more, questions still remain as to their etiology. We quantified antibody levels to pathogens that potentially contribute to ADL episodes during and after an episode among lymphedema patients in Léogâne, Haiti. We estimated the proportion of ADL episodes hypothesized to be attributed to specific pathogens.We measured antibody levels to specific pathogens during and following an ADL episode among 41 lymphedema patients enrolled in a cohort study in Léogâne, Haiti. We calculated the absolute and relative changes in antibody levels between the ADL and convalescent time points. We calculated the proportion of episodes that demonstrated a two-fold increase in antibody level for several bacterial, fungal, and filarial pathogens.Our results showed the greatest proportion of two-fold changes in antibody levels for the carbohydrate antigen Streptococcus group A, followed by IgG2 responses to a soluble filarial antigen (BpG2, Streptococcal Pyrogenic Exotoxin B, and an antigen for the fungal pathogen Candida. When comparing the median antibody level during the ADL episode to the median antibody level at the convalescent time point, only the antigens for Pseudomonas species (P-value = 0.0351 and Streptolysin O (P-value = 0.0074 showed a significant result.Although our results are limited by the lack of a control group and few antibody responses, they provide some evidence for infection with Streptococcus A as a potential contributing factor to ADL episodes. Our results add to the current evidence and illustrate the importance of determining the causal role of bacterial and fungal pathogens and immunological antifilarial response in ADL episodes.

  4. Serum and urinary interleukin-6 (IL-6) levels as predicting factors of Kawasaki disease activity.

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    Chow, Y M; Lin, C Y; Hwang, B

    1993-01-01

    From January 1988 to Autumn 1991, 60 patients suffering from Kawasaki disease (KD) were recruited in this study. Their ages ranged from 4 months to 5 years. Diagnosis was based on the criteria revised in 1984 by the KD Research Committee in Japan. Of these, 12 cases developed coronary aneurysms. First, blood samples from 60 KD patients were taken on admission before aspirin and/or intravenous immunoglobulin (IVIG) treatment. Convalescent blood samples were taken 3 months after onset of disease. The control group included (1) 10 cases of viral infection with skin rash and fever (aged 5 months to 5 years) and (2) 10 age and sex matched normal children admitted for elective pediatric surgery such as inguinal hernia. Second, urinary samples were collected from 32 cases during the acute phase of KD. Of these, 10 cases had pyuria and/or proteinuria. The results showed that the serum IL-6 levels from KD patients during the first week of acute phase were significantly increased while undetectable in the convalescent sera and controls. There was also a statistical difference between the with and without coronary aneurysm groups during the first week (336.8 +/- 95.1 vs 125.5 +/- 56.5 pg/ml, P Urinary IL-6 levels were significantly elevated in KD patients with pyuria and/or proteinuria (156.6 +/- 77.7 pg/mg Cr) and undetectable in the group without pyuria and proteinuria and controls during the first week. There was no difference between with and without coronary aneurysm. These results suggest that serum IL-6 level is a useful factor for predicting formation of coronary aneurysm even within one week after onset of disease.(ABSTRACT TRUNCATED AT 250 WORDS)

  5. Affects of N-terminal variation in the SeM protein of Streptococcus equi on antibody and fibrinogen binding.

    Science.gov (United States)

    Timoney, John F; DeNegri, Rafaela; Sheoran, Abhineet; Forster, Nathalie

    2010-02-10

    The clonal Streptococcus equi causes equine strangles, a highly contagious suppurative lymphadenopathy and rhinopharyngitis. An important virulence factor and vaccine component, the antiphagocytic fibrinogen binding SeM of S. equi is a surface anchored fibrillar protein. Two recent studies of N. American, Japanese and European isolates have revealed a high frequency of N-terminal amino acid variation in SeM of S. equi CF32 that suggests this region of the protein is subject to immunologic selection pressure. The aims of the present study were firstly to map regions of SeM reactive with convalescent equine IgG and IgA and stimulatory for lymph node cells and secondly to determine effects of N-terminal variation on the functionality of SeM. Variation did not significantly affect fibrinogen binding or susceptibility of S. equi to an opsonic equine serum. Linear epitopes reactive with convalescent IgG and mucosal IgA were concentrated toward the conserved center of SeM. However, IgA but not IgG from every horse reacted with at least one peptide that contained variable sequence. Lymph node cells (CD4+) from horses immunized with SeM were strongly responsive to a peptide (alphaalpha36-138) encoding the entire variable region. SeM (CF32) specific mouse Mab 04D11 which reacted strongly with this larger peptide but not with shorter peptides within that sequence reacted strongly with whole cells of S. equi CF32 but only weakly with cells of any of 14 isolates of S. equi expressing different variants of SeM. These results in combination suggest that N-terminal variation alters a conformational epitope of significance in mucosal IgA and systemic T cell responses but does not affect antibody mediated phagocytosis and killing.

  6. The application of correction factors on serum retinol of Indonesia school children

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    Drupadi Dillon

    2010-11-01

    Full Text Available Aim: Vitamin A deficiency among children is still a public health problem in Indonesia. Serum/plasma retinol concentration is the best indicator in assessing vitamin A status. However, there is growing concern that infection/ inflammation lowers serum  retinol concentration, thus creating potential misinterpretation of vitamin A status, which could affect policy makers in planning suitable nutrition programs targeted at community. The aim of this study was to highlight the importance of applying correction factors, to better interpret serum retinol as a nutritional statusbiomarker.Methods: A cross sectional study involving 54 apparently healthy school children was conducted in East Nusa Tenggara. Height, body weight, concentrations of serum retinol, CRP and AGP were assessed. Based on concentrations of serum CRP and AGP, four infl ammation groups were determined, namely reference,  incubation, early convalescence and late convalescence groups. Correction factor was obtained by dividing serum retinol  concentration of reference group by that of the other three groups. Correction factors were then used to correct serum retinol concentration without any  influence of infection/ inflammation.Results: The prevalence of stunting and underweight were 43% and 22% respectively, but there was no wasting among the school children. Applying correction factor lowered the prevalence of vitamin A defi ciency from 20.4% to 18.5%; thus changing vitamin A defi ciency from a severe public health problem to a moderate public health problem.Conclusion: Correcting serum retinol concentration for the infl uence of infection reduced the apparent severity of vitamin A deficiency. This could affect policy for planning nutrition programs designed for communities. (Med J Indones 2010;  19:258-63Keywords: correction factor, infection, school children, serum retinol

  7. Serum metabolome and lipidome changes in adult patients with primary dengue infection.

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    Liang Cui

    Full Text Available BACKGROUND: Dengue virus (DENV is the most widespread arbovirus with an estimated 100 million infections occurring every year. Endemic in the tropical and subtropical areas of the world, dengue fever/dengue hemorrhagic fever (DF/DHF is emerging as a major public health concern. The complex array of concurrent host physiologic changes has hampered a complete understanding of underlying molecular mechanisms of dengue pathogenesis. METHODOLOGY/PRINCIPLE FINDINGS: Systems level characterization of serum metabolome and lipidome of adult DF patients at early febrile, defervescence, and convalescent stages of DENV infection was performed using liquid chromatography- and gas chromatography-mass spectrometry. The tractability of following metabolite and lipid changes in a relatively large sample size (n = 44 across three prominent infection stages allowed the identification of critical physiologic changes that coincided with the different stages. Sixty differential metabolites were identified in our metabolomics analysis and the main metabolite classes were free fatty acids, acylcarnitines, phospholipids, and amino acids. Major perturbed metabolic pathways included fatty acid biosynthesis and β-oxidation, phospholipid catabolism, steroid hormone pathway, etc., suggesting the multifactorial nature of human host responses. Analysis of phospholipids and sphingolipids verified the temporal trends and revealed association with lymphocytes and platelets numbers. These metabolites were significantly perturbed during the early stages, and normalized to control levels at convalescent stage, suggesting their potential utility as prognostic markers. CONCLUSIONS/SIGNIFICANCE: DENV infection causes temporally distinct serum metabolome and lipidome changes, and many of the differential metabolites are involved in acute inflammatory responses. Our global analyses revealed early anti-inflammatory responses working in concert to modulate early pro-inflammatory processes

  8. Surveillance of chikungunya virus inAndhraPradesh,Southern India

    Institute of Scientific and Technical Information of China (English)

    CVMNareshKumar; P Sangamithra; M Rajasekhar; DVR Saigopal

    2010-01-01

    Objective:The study involved survey and screening of areas suspected of chikungunya virus (CHIKV)infection, characterizing the causative agent and identifying the circulatingCHIKV genotype.Methods: Acute phase samples were screened by the use ofRTPCR using primer setDVRChk-F/DVRChk-R whereas convalescent samples were tested byCHIKV IgM strips. Results: Two hundred and seventy five acute phase samples were screened by RT-PCR, of which 149(54.18%) showed positivity forCHIKV. Later on192 convalescent phase samples were tested forCHIKV specific antibodies in which125(65.10%) samples were found to be positive. Four CHIKV strains were selected and subjected to cloning followed by nucleotide sequencing and were submitted to the GenbankDNAdatabase with the Accession numbers(GQ119362,GQ119363, GQ119364, andFJ225403). The Sequence analysis of“CHIK-Kadapa” strain(GQ119362)with other CHIKV isolates suggested that the present CHIKV strain has (99.23± 0.52) % and100 %identity with Central East South African isolates(CESA) at nucleotide and amino acid levels respectively. Two unique non synonymous mutations S168L andD183Vwere depicted in E1 gene of the selected strains of the present study.Conclusions:The14 months survey revealed the circulation of CHIKV in2008-2009in Andhra Pradesh and the causative agent is identified to be of Central East South African(CESA) origin. The importance of the non synonymous mutations (S168L and D183V) and their role in the mobility and strength of theE1-E1andE1-E2 interactions needs further investigations. The study also urges the need for intensifying the epidemiological and entomological surveillance to combat any suchCHIKV outbreak in the near future.

  9. High pro-inflammatory cytokine secretion and loss of high avidity cross-reactive cytotoxic T-cells during the course of secondary dengue virus infection.

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    Tao Dong

    Full Text Available BACKGROUND: Dengue is one of the most important human diseases transmitted by an arthropod vector and the incidence of dengue virus infection has been increasing - over half the world's population now live in areas at risk of infection. Most infections are asymptomatic, but a subset of patients experience a potentially fatal shock syndrome characterised by plasma leakage. Severe forms of dengue are epidemiologically associated with repeated infection by more than one of the four dengue virus serotypes. Generally attributed to the phenomenon of antibody-dependent enhancement, recent observations indicate that T-cells may also influence disease phenotype. METHODS AND FINDINGS: Virus-specific cytotoxic T lymphocytes (CTL showing high level cross reactivity between dengue serotypes could be expanded from blood samples taken during the acute phase of secondary dengue infection. These could not be detected in convalescence when only CTL populations demonstrating significant serotype specificity were identified. Dengue cross-reactive CTL clones derived from these patients were of higher avidity than serotype-specific clones and produced much higher levels of both type 1 and certain type 2 cytokines, many previously implicated in dengue pathogenesis. CONCLUSION: Dengue serotype cross-reactive CTL clones showing high avidity for antigen produce higher levels of inflammatory cytokines than serotype-specific clones. That such cells cannot be expanded from convalescent samples suggests that they may be depleted, perhaps as a consequence of activation-induced cell death. Such high avidity cross-reactive memory CTL may produce inflammatory cytokines during the course of secondary infection, contributing to the pathogenesis of vascular leak. These cells appear to be subsequently deleted leaving a more serotype-specific memory CTL pool. Further studies are needed to relate these cellular observations to disease phenotype in a large group of patients. If

  10. The expression of REG 1A and REG 1B is increased during acute amebic colitis.

    Science.gov (United States)

    Peterson, Kristine M; Guo, Xiaoti; Elkahloun, Abdel G; Mondal, Dinesh; Bardhan, Pradip K; Sugawara, Akira; Duggal, Priya; Haque, Rashidul; Petri, William A

    2011-09-01

    Entamoeba histolytica, a protozoan parasite, is an important cause of diarrhea and colitis in the developing world. Amebic colitis is characterized by ulceration of the intestinal mucosa. We performed microarray analysis of intestinal biopsies during acute and convalescent amebiasis in order to identify genes potentially involved in tissue injury or repair. Colonic biopsy samples were obtained from 8 patients during acute E. histolytica colitis and again 60 days after recovery. Gene expression in the biopsies was evaluated using microarray, and confirmed by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). REG 1A and REG 1B were the most up-regulated of all genes in the human intestine in acute versus convalescent E. histolytica disease: as determined by microarray, the levels of induction were 7.4-fold and 10.7 fold for REG 1A and B; p=0.003 and p=0.006 respectively. Increased expression of REG 1A and REG 1B protein in the colonic crypt epithelial cells during acute amebiasis was similarly observed by immunohistochemistry. Because REG 1 protein is anti-apoptotic and pro-proliferative, and since E. histolytica induces apoptosis of the intestinal epithelium as part of its disease process, we next tested if REG 1 might be protective during amebiasis by preventing parasite-induced apoptosis. Intestinal epithelial cells from REG 1-/- mice were found to be more susceptible to spontaneous, and parasite-induced, apoptosis in vitro (p=0.03). We concluded that REG 1A and REG 1B were upregulated during amebiasis and may function to protect the intestinal epithelium from parasite-induced apoptosis. Published by Elsevier Ireland Ltd.

  11. Sequential waves of gene expression in patients with clinically defined dengue illnesses reveal subtle disease phases and predict disease severity.

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    Peifang Sun

    Full Text Available BACKGROUND: Dengue virus (DENV infection can range in severity from mild dengue fever (DF to severe dengue hemorrhagic fever (DHF or dengue shock syndrome (DSS. Changes in host gene expression, temporally through the progression of DENV infection, especially during the early days, remains poorly characterized. Early diagnostic markers for DHF are also lacking. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we investigated host gene expression in a cohort of DENV-infected subjects clinically diagnosed as DF (n = 51 and DHF (n = 13 from Maracay, Venezuela. Blood specimens were collected daily from these subjects from enrollment to early defervescence and at one convalescent time-point. Using convalescent expression levels as baseline, two distinct groups of genes were identified: the "early" group, which included genes associated with innate immunity, type I interferon, cytokine-mediated signaling, chemotaxis, and complement activity peaked at day 0-1 and declined on day 3-4; the second "late" group, comprised of genes associated with cell cycle, emerged from day 4 and peaked at day 5-6. The up-regulation of innate immune response genes coincided with the down-regulation of genes associated with viral replication during day 0-3. Furthermore, DHF patients had lower expression of genes associated with antigen processing and presentation, MHC class II receptor, NK and T cell activities, compared to that of DF patients. These results suggested that the innate and adaptive immunity during the early days of the disease are vital in suppressing DENV replication and in affecting outcome of disease severity. Gene signatures of DHF were identified as early as day 1. CONCLUSIONS/SIGNIFICANCE: Our study reveals a broad and dynamic picture of host responses in DENV infected subjects. Host response to DENV infection can now be understood as two distinct phases with unique transcriptional markers. The DHF signatures identified during day 1-3 may have

  12. Clinical significance of matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 and 2 in Kawasaki disease

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    Ki Wook Yun

    2010-04-01

    Full Text Available Purpose : Kawasaki disease (KD is a systemic vasculitis, a leading cause of pediatric acquired heart disease. Histopathological findings of coronary artery lesion (CAL in KD indicate destruction of the coronary artery wall with diffuse vasculitis. Matrix metalloproteinases (MMPs and their endogenous tissue inhibitors (TIMPs might play central roles in this process. Special attention to MMP-9 has recently been emerging. This study was performed to investigate the clinical significance of MMP-9 and its inhibitors, TIMP-1 and TIMP-2, in KD. Methods : We compared 47 KD patients with 14 febrile controls. Serum MMP-9 and TIMP-1, TIMP-2 were measured by ELISA and compared according to clinical stages and coronary involvement. Results : In acute stage, MMP-9 and TIMP-1 were significantly higher, whereas TIMP-2 was lower, in KD than those in febrile controls (P &lt;0.05. The elevated MMP-9 levels in acute phase significantly decreased during the subacute and convalescent phases (P &lt;0.05. During acute phase, the MMP-9, TIMP-1, and MMP-9/TIMP-2 levels in the CAL group were lower than those in the non-CAL group, but they increased significantly in the subacute phase (P &lt;0.05. MMP-9 has a positive correlation with TIMP-1 in the acute and subacute phases, and negative correlation with TIMP-2 in the subacute and convalescent phases (P &lt;0.05. Conclusion : These results suggest that MMP-9, TIMP-1, and the imbalance in MMP-9 and TIMP-2 might play important roles on the pathophysiology of KD and especially on the development of CAL. However, further larger studies are needed.

  13. A comparison of the toxinological characteristics of two Cassiopea and Aurelia species.

    Science.gov (United States)

    Radwan, F F; Burnett, J W; Bloom, D A; Coliano, T; Eldefrawi, M E; Erderly, H; Aurelian, L; Torres, M; Heimer-de la Cotera, E P

    2001-01-01

    A comparison of the toxinological properties of nematocyst venoms from Old and New World Cassiopea and Aurelia species was undertaken. The cnidom of venomous Cassiopea andromeda (Ca) and Aurelia (Aa(RS)) from the Red Sea was identical to that of nonvenomous Bahamian Cassiopea xamancha (Cx) and Chesapeake Bay Aurelia aurita (Aa(CB)), respectively. A clean nematocyst preparation of Ca and both Aurelias could be obtained but algal particles could not be separated completely from the Cx nematocysts. Further purification of all four nematocyst preparations showed significant differences in the action of their protein. Only the Cassiopea had coexisting dermonecrotic and vasopermeability producing properties and Ca's hemolytic activity was associated with mouse lethality. The protein, hemolysin and phospholipase gel filtration eluant curves of Ca venom were similar. Venomous Aa(RS) actively stung lips and contained more potent mouse lethal, demonecrotic, vasopermeability plus hemolytic factors than Aa(CB). Cross reactivity of convalescent human serum obtained from patients stung by Ca and venomous Cx collected in Central America occurred. This was also observed between sera of bathers stung by Aa(RS) and stinging Aurelia which appeared in Florida during the recent El Niño year. IgG was stimulated by several nematocyst proteins since many venom subfractions tested positive at high titers against convalescent sera. T-cell proliferation of mice primed with either Aurelia venom was positive against the homologous preparation with cross reactivity to the heterologous venom. Crude venoms of both Red Sea jellyfish metabolically stimulated cultured human hepatocytes more than their New World counterparts. This data shows that considerable similarities and differences exist in the venoms of these Old and New World Cassiopea and Aurelia medusae with the Eastern species being more potent.

  14. Evaluation of a Western Blot Test in an Outbreak of Acute Pulmonary Histoplasmosis

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    Pizzini, Claudia V.; Zancopé-Oliveira, Rosely M.; Reiss, Errol; Hajjeh, Rana; Kaufman, Leo; Peralta, José Mauro

    1999-01-01

    A western blot (WB) test was evaluated for detection of antibodies against native glycosylated and chemically deglycosylated M and H antigens of Histoplasma capsulatum in serum obtained from patients during the acute phase of pulmonary histoplasmosis that occurred during an outbreak. Of 275 serum samples tested by immunodiffusion and complement fixation (CF) samples from 40 patients affected during this outbreak and from 37 negative controls were tested by WB test. A group of patients whose sera were negative for CF antibodies and precipitins early in the acute stage of histoplasmosis but who all seroconverted during convalescence 6 weeks later were tested with the WB test. Antibodies against untreated H and M antigens were detected at a 1:100 dilution by WB test in 45% of the 20 acute-phase serum samples and in all 20 of the convalescent-phase specimens. The WB test’s sensitivity for acute-phase specimens increased to 90% (18 of 20 specimens) when H and M antigens were treated by periodate oxidation to inactivate susceptible carbohydrate epitopes. When native glycosylated antigens were used in the WB test, positive reactions were observed in negative control serum specimens (3 of 37 specimens; 8%) and in serum specimens obtained from asymptomatic persons screened as part of the outbreak investigation (13 of 20 specimens; 65%). These positive reactions were also attributed to glycosidic epitopes since the specificity of the WB test increased from 78 to 100% when periodate-treated H and M antigens were used. WB test with deglycosylated H and M antigens of histoplasmin provides a rapid, sensitive, and specific test to diagnose acute pulmonary histoplasmosis before precipitins can be detected. PMID:9874658

  15. Serum Metabolomics Reveals Serotonin as a Predictor of Severe Dengue in the Early Phase of Dengue Fever.

    Science.gov (United States)

    Cui, Liang; Lee, Yie Hou; Thein, Tun Linn; Fang, Jinling; Pang, Junxiong; Ooi, Eng Eong; Leo, Yee Sin; Ong, Choon Nam; Tannenbaum, Steven R

    2016-04-01

    Effective triage of dengue patients early in the disease course for in- or out-patient management would be useful for optimal healthcare resource utilization while minimizing poor clinical outcome due to delayed intervention. Yet, early prognosis of severe dengue is hampered by the heterogeneity in clinical presentation and routine hematological and biochemical measurements in dengue patients that collectively correlates poorly with eventual clinical outcome. Herein, untargeted liquid-chromatography mass spectrometry metabolomics of serum from patients with dengue fever (DF) and dengue hemorrhagic fever (DHF) in the febrile phase (1.5) in the serum, among which are two products of tryptophan metabolism-serotonin and kynurenine. Serotonin, involved in platelet aggregation and activation decreased significantly, whereas kynurenine, an immunomodulator, increased significantly in patients with DHF, consistent with thrombocytopenia and immunopathology in severe dengue. To sensitively and accurately evaluate serotonin levels as prognostic biomarkers, we implemented stable-isotope dilution mass spectrometry and used convalescence samples as their own controls. DHF serotonin was significantly 1.98 fold lower in febrile compared to convalescence phase, and significantly 1.76 fold lower compared to DF in the febrile phase of illness. Thus, serotonin alone provided good prognostic utility (Area Under Curve, AUC of serotonin = 0.8). Additionally, immune mediators associated with DHF may further increase the predictive ability than just serotonin alone. Nine cytokines, including IFN-γ, IL-1β, IL-4, IL-8, G-CSF, MIP-1β, FGF basic, TNFα and RANTES were significantly different between DF and DHF, among which IFN-γ ranked top by multivariate statistics. Combining serotonin and IFN-γ improved the prognosis performance (AUC = 0.92, sensitivity = 77.8%, specificity = 95.8%), suggesting this duplex panel as accurate metrics for the early prognosis of DHF.

  16. COMPARATIVE STUDY OF LICHTENSTEIN VERSUS DESARDA REPAIR FOR INGUINAL HERNIA

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    Sowmya

    2015-12-01

    Full Text Available BACKGROUND Inguinal hernia repair is the most frequently performed operation in any general surgical unit. The Bassini’s, Shouldice and other tissue-based techniques are still being acceptable for primary inguinal hernia repair. Desarda’s technique is originally a tissue based hernia repair using an undetached strip of external oblique aponeurosis to strengthen the posterior wall of the inguinal canal. The aim of the present study was to compare Lichtenstein hernia repair and Desarda herniorrhaphy. METHODS A total of 40 patients with primary unilateral inguinal hernia were subjected either to Desarda herniorrhaphy or Lichtenstein hernioplasty. The patients were followed in terms of recurrence rate, post-operative complications, convalescence, chronic pain and cost effectiveness. RESULTS During the followup all patients had either mild or moderate pain, but the pain intensity was more in Lichtenstein repair compared to Desarda repair in the immediate postoperative period. In Lichenstein repair patients had chronic groin pain even at the end of one year, but none of the patients in Desarda repair had chronic groin pain. Complications such as seroma and wound infection were less in Desarda repair. Time taken to resume normal activities was significantly less in case of Desarda herniorrhaphy; however, there was no recurrence observed in both the groups during the followup period. Average cost incurred for Desarda repair was significantly less than Lichtenstein repair. CONCLUSION Lichtenstein method of hernia repair is simple and safe. But the mesh prosthesis has its drawbacks. Desarda hernia repair is based on physiological principles and the results are good with less convalescence period and fewer recurrences and no chronic groin pain. It is more cost effective.

  17. The putative capsid protein of the newly identified avian hepatitis E virus shares antigenic epitopes with that of swine and human hepatitis E viruses and chicken big liver and spleen disease virus.

    Science.gov (United States)

    Haqshenas, G; Huang, F F; Fenaux, M; Guenette, D K; Pierson, F W; Larsen, C T; Shivaprasad, H L; Toth, T E; Meng, X J

    2002-09-01

    We recently identified a novel virus, designated avian hepatitis E virus (avian HEV), from chickens with hepatitis-splenomegaly (HS) syndrome in the USA. We showed that avian HEV is genetically related to swine and human HEVs. Here we report the antigenic cross-reactivity of the putative open reading frame 2 (ORF2) capsid protein of avian HEV with those of swine and human HEVs and the Australian chicken big liver and spleen disease virus (BLSV). The region encoding the C-terminal 268 amino acid residues of avian HEV ORF2 was cloned into expression vector pRSET-C. The truncated ORF2 protein was expressed in E. coli as a fusion protein and purified by affinity chromatography. Western blot analysis revealed that the avian HEV ORF2 protein reacted with antisera against the Sar-55 strain of human HEV and with convalescent antisera against swine HEV and the US2 strain of human HEV, as well as with antiserum against BLSV. Convalescent sera from specific-pathogen-free chickens experimentally infected with avian HEV also reacted with the recombinant capsid proteins of swine HEV and Sar-55 human HEV. Antisera against the US2 human HEV also reacted with recombinant ORF2 proteins of both swine HEV and Sar-55 human HEV. The antigenic cross-reactivity of the avian HEV putative capsid protein with those of swine and human HEVs was further confirmed, for the most part, by ELISA assays. The data indicate that avian HEV shares certain antigenic epitopes in its putative capsid protein with swine and human HEVs, as well as with BLSV. The results have implications for HEV diagnosis and taxonomy.

  18. Multiplex immunoassay for Lyme disease using VlsE1-IgG and pepC10-IgM antibodies: improving test performance through bioinformatics.

    Science.gov (United States)

    Porwancher, Richard B; Hagerty, C Greg; Fan, Jianqing; Landsberg, Lisa; Johnson, Barbara J B; Kopnitsky, Mark; Steere, Allen C; Kulas, Karen; Wong, Susan J

    2011-05-01

    The Centers for Disease Control and Prevention currently recommends a 2-tier serologic approach to Lyme disease laboratory diagnosis, comprised of an initial serum enzyme immunoassay (EIA) for antibody to Borrelia burgdorferi followed by supplementary IgG and IgM Western blotting of EIA-positive or -equivocal samples. Western blot accuracy is limited by subjective interpretation of weakly positive bands, false-positive IgM immunoblots, and low sensitivity for detection of early disease. We developed an objective alternative second-tier immunoassay using a multiplex microsphere system that measures VlsE1-IgG and pepC10-IgM antibodies simultaneously in the same sample. Our study population comprised 79 patients with early acute Lyme disease, 82 patients with early-convalescent-phase disease, 47 patients with stage II and III disease, 34 patients post-antibiotic treatment, and 794 controls. A bioinformatic technique called partial receiver-operator characteristic (ROC) regression was used to combine individual antibody levels into a single diagnostic score with a single cutoff; this technique enhances test performance when a high specificity is required (e.g., ≥ 95%). Compared to Western blotting, the multiplex assay was equally specific (95.6%) but 20.7% more sensitive for early-convalescent-phase disease (89.0% versus 68.3%, respectively; 95% confidence interval [95% CI] for difference, 12.1% to 30.9%) and 12.5% more sensitive overall (75.0% versus 62.5%, respectively; 95% CI for difference, 8.1% to 17.1%). As a second-tier test, a multiplex assay for VlsE1-IgG and pepC10-IgM antibodies performed as well as or better than Western blotting for Lyme disease diagnosis. Prospective validation studies appear to be warranted.

  19. Multiplex Immunoassay for Lyme Disease Using VlsE1-IgG and pepC10-IgM Antibodies: Improving Test Performance through Bioinformatics ▿

    Science.gov (United States)

    Porwancher, Richard B.; Hagerty, C. Greg; Fan, Jianqing; Landsberg, Lisa; Johnson, Barbara J. B.; Kopnitsky, Mark; Steere, Allen C.; Kulas, Karen; Wong, Susan J.

    2011-01-01

    The Centers for Disease Control and Prevention currently recommends a 2-tier serologic approach to Lyme disease laboratory diagnosis, comprised of an initial serum enzyme immunoassay (EIA) for antibody to Borrelia burgdorferi followed by supplementary IgG and IgM Western blotting of EIA-positive or -equivocal samples. Western blot accuracy is limited by subjective interpretation of weakly positive bands, false-positive IgM immunoblots, and low sensitivity for detection of early disease. We developed an objective alternative second-tier immunoassay using a multiplex microsphere system that measures VlsE1-IgG and pepC10-IgM antibodies simultaneously in the same sample. Our study population comprised 79 patients with early acute Lyme disease, 82 patients with early-convalescent-phase disease, 47 patients with stage II and III disease, 34 patients post-antibiotic treatment, and 794 controls. A bioinformatic technique called partial receiver-operator characteristic (ROC) regression was used to combine individual antibody levels into a single diagnostic score with a single cutoff; this technique enhances test performance when a high specificity is required (e.g., ≥95%). Compared to Western blotting, the multiplex assay was equally specific (95.6%) but 20.7% more sensitive for early-convalescent-phase disease (89.0% versus 68.3%, respectively; 95% confidence interval [95% CI] for difference, 12.1% to 30.9%) and 12.5% more sensitive overall (75.0% versus 62.5%, respectively; 95% CI for difference, 8.1% to 17.1%). As a second-tier test, a multiplex assay for VlsE1-IgG and pepC10-IgM antibodies performed as well as or better than Western blotting for Lyme disease diagnosis. Prospective validation studies appear to be warranted. PMID:21367982

  20. Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of Borrelia burgdorferi vlsE.

    Science.gov (United States)

    Liang, F T; Steere, A C; Marques, A R; Johnson, B J; Miller, J N; Philipp, M T

    1999-12-01

    VlsE, the variable surface antigen of Borrelia burgdorferi, contains an immunodominant conserved region named IR(6). In the present study, the diagnostic performance of a peptide enzyme-linked immunosorbent assay (ELISA) based on a 26-mer synthetic peptide (C(6)) with the IR(6) sequence was explored. Sensitivity was assessed with serum samples (n = 210) collected from patients with clinically defined Lyme disease at the acute (early localized or early disseminated disease), convalescent, or late disease phase. The sensitivities for acute-, convalescent-, and late-phase specimens were 74% (29 of 39), 85 to 90% (34 of 40 to 35 of 39), and 100% (59 of 59), respectively. Serum specimens from early neuroborreliosis patients were 95% positive (19 of 20), and those from an additional group of patients with posttreatment Lyme disease syndrome yielded a sensitivity of 62% (8 of 13). To assess the specificity of the peptide ELISA, 77 serum samples from patients with other spirochetal or chronic infections, autoimmune diseases, or neurologic diseases and 99 serum specimens from hospitalized patients in an area where Lyme disease is not endemic were examined. Only two potential false positives from the hospitalized patients were found, and the overall specificity was 99% (174 of 176). Precision, which was assessed with a panel of positive and negative serum specimens arranged in blinded duplicates, was 100%. Four serum samples with very high anti-OspA antibody titers obtained from four monkeys given the OspA vaccine did not react with the C(6) peptide. This simple, sensitive, specific, and precise ELISA may contribute to alleviate some of the remaining problems in Lyme disease serodiagnosis. Because of its synthetic peptide base, it will be inexpensive to manufacture. It also will be applicable to serum specimens from OspA-vaccinated subjects.

  1. 川崎病患儿血清白介素-17的变化%The changes of serum IL-17 in children with Kawasaki disease

    Institute of Scientific and Technical Information of China (English)

    李蕴言; 吴菱; 马田瑞; 陈圆玲; 狄亚珍

    2013-01-01

    Objectives To explore the change of interleukin-17 (IL-17) in Kawasaki disease (KD). Methods Fourty KD pediatric patients, among them 12 patients with echocardiographic abnormalities in acute phase, 25 age-matched non-KD patients were enrolled. The level of serum IL-17 was measured by enzyme linked immunosorbent assay in acute and convalescent phase of KD patients and non-KD patients. At the same time, C-reactive protein (CRP), globulin, albumin were detected. Results In acute phase of KD patients, the level of serum IL-17 were signiifcantly higher than that in convalescent phase of KD patients and non-KD patients (P0.05). In acute phase of KD patients with echocardiography abnormalities, the level of serum IL-17 was signiifcantly higher than that with non-echocardiography abnormalities (P0.05);急性期超声心动图异常KD患儿的血清IL-17水平高于超声心动图正常的患儿,差异有统计学意义(P<0.05)。KD患儿急性期血清IL-17与CRP、球蛋白水平呈正相关(r=0.750、0.750,P均<0.05),而与白蛋白水平呈负相关(r=-0.779, P<0.05)。结论 IL-17参与KD的免疫损伤过程,血清IL-17水平是KD活动的指标之一,IL-17水平与心血管损害相关。

  2. SARS: systematic review of treatment effects.

    Directory of Open Access Journals (Sweden)

    Lauren J Stockman

    2006-09-01

    Full Text Available BACKGROUND: The SARS outbreak of 2002-2003 presented clinicians with a new, life-threatening disease for which they had no experience in treating and no research on the effectiveness of treatment options. The World Health Organization (WHO expert panel on SARS treatment requested a systematic review and comprehensive summary of treatments used for SARS-infected patients in order to guide future treatment and identify priorities for research. METHODS AND FINDINGS: In response to the WHO request we conducted a systematic review of the published literature on ribavirin, corticosteroids, lopinavir and ritonavir (LPV/r, type I interferon (IFN, intravenous immunoglobulin (IVIG, and SARS convalescent plasma from both in vitro studies and in SARS patients. We also searched for clinical trial evidence of treatment for acute respiratory distress syndrome. Sources of data were the literature databases MEDLINE, EMBASE, BIOSIS, and the Cochrane Central Register of Controlled Trials (CENTRAL up to February 2005. Data from publications were extracted and evidence within studies was classified using predefined criteria. In total, 54 SARS treatment studies, 15 in vitro studies, and three acute respiratory distress syndrome studies met our inclusion criteria. Within in vitro studies, ribavirin, lopinavir, and type I IFN showed inhibition of SARS-CoV in tissue culture. In SARS-infected patient reports on ribavirin, 26 studies were classified as inconclusive, and four showed possible harm. Seven studies of convalescent plasma or IVIG, three of IFN type I, and two of LPV/r were inconclusive. In 29 studies of steroid use, 25 were inconclusive and four were classified as causing possible harm. CONCLUSIONS: Despite an extensive literature reporting on SARS treatments, it was not possible to determine whether treatments benefited patients during the SARS outbreak. Some may have been harmful. Clinical trials should be designed to validate a standard protocol for dosage

  3. Comparison of nasopharyngeal culture, polymerase chain reaction (PCR) and serological test for diagnosis of pertussis.

    Science.gov (United States)

    Cengiz, Ali Bülent; Yildirim, Inci; Ceyhan, Mehmet; Seçmeer, Gülten; Gür, Deniz; Kara, Ateş

    2009-01-01

    This prospective study, which was designed to compare nasopharyngeal culture, polymerase chain reaction (PCR) and serology in the diagnosis of pertussis, covered 35 children aged between 0 and 16 who were admitted to Hacettepe University Ihsan Doğramaci Children's Hospital between 1 March 2005 and 31 August 2006 with coughing for 7 days or longer, paroxysmal cough of any duration, or cough with inspiratory whoop and/or vomiting (or apnea) after coughs. The demographic data and vaccination history of the patients were recorded. During the initial examination, samples were taken from the posterior nasopharynx for Bordetella pertussis (B. pertussis) culture and PCR analysis. In order to determine antibody positivity and antibody levels against B. pertussis antigens, serum samples were taken during the initial examination (acute phase) and two weeks later (convalescent phase). In the first serum sample, immunoglobulin M (IgM) was determined against pertussis toxin. In the first and second samples, IgA and IgG antibodies were evaluated against pertussis toxin and filamentous hemagglutinin. Culture yielded negative results in all of the patients. PCR was positive in two cases (5.7%). In the PCR-positive patients, IgM, IgA and IgG type anti-pertussis antibodies were found to be positive in the first serum samples, and IgA and IgG antibodies were found to be positive in the second serum samples. Therefore, it was considered that serology could be as sensitive as PCR when type IgM, IgA and IgG antibodies were found to be positive against a minimum of two antigens of B. pertussis. In conclusion, both PCR and serologic tests--if evaluating all types of antibodies to a minimum of two antigens of B. pertussis obtained in both acute and convalescent sera--could be more sensitive than culture in the diagnosis of pertussis.

  4. Effect of low-dose erythromycin estolate on sputum neutrophils and serum interleukin-8, interleukin-17 in children with asthma%小剂量依托红霉素对哮喘患儿痰液中性粒细胞及血清白介素-8、白介素-17的影响

    Institute of Scientific and Technical Information of China (English)

    涂国华; 钱金强; 易阳; 季德成; 熊建新; 丁翠君; 钱琴芬; 邓素丹; 王丽晴

    2012-01-01

    Objective To explore the effeet of low-dose erythromycin estolate on sputum neutrophils (PMN) and serum levels of interleukin-8 and interleukin-17 in children with asthma. Methods From January 2007 to January 2009, 24 children with asthma were randomly divided into non-erythromycin eslolate group and erythromycin estolate group, twelve children for each group. Two groups of children received regular treatment including regular inhaled corticosteroids and intermittent inhaled B2 agonists, meanwhile erythromyoin estolate group was taken orally erythromyoin estolate 3-5 mg/(kg·d) for four weeks. During both nf the acute and convalescent periods, cell sorting from induced sputum was processed , the serum conentratibDs of IL-8 and IL-17 were tested by ELISA and lung functions were detected in the two groups. Results In two groups, the PMN of induced sputum in convaleseent period was significantly lower than that in acute period (P < 0.001). The PMN in convalescent period was significantly lower in erythromycin estolate group than that in non-erythromycin estolate group (P= 0.001). The serum levels of IL-8 and IL-17 in two groups in convalescent period were significantly lower than those of during acute period (P convalescent period (P < 0.01). The lung functions were improved significantly testing in two groups during convalescent period than those during acute time (P< 0.001), and the improvement rate ( △PEFR%) in erythromycin estolate groupwas significantly greater than that in non-erythromycin estolate group (Z = 3.349; P= 0.001). Conclusions Low-dose erythromycin estolate can significantly improve airway inflammation and lung function in children with asthma, thus may be a new way of treatment for asthma.%目的 探讨小剂量依托红霉素对哮喘患儿痰液中性粒细胞(PMN)及血清白介素-8(IL-8)

  5. Establishment and application of neutralization assay based on H5N1 avian influenza pseudotyped virus in vitro%H5N1禽流感假病毒体外中和试验技术平台的建立与应用

    Institute of Scientific and Technical Information of China (English)

    张国良; 周伯平; 蔡车国; 陈心春; 杨桂林; 陆坚; 聂广; 周保罗

    2011-01-01

    目的 建立H5N1禽流感假病毒的体外中和试验技术平台,并系统评价2份H5N1人禽流感患者恢复期血清的中和效价.方法 通过磷酸钙沉淀法将转移载体pHR-Luc、包装载体pCMV△8.2和包膜载体CMV/R-SZ(或CMV/R-TH)共转染人胚肾细胞(293T),72 h后收集假病毒上清液,免疫印迹(Western blot)鉴定假病毒颗粒HA、P24蛋白的表达,并取200μL假病毒上清液测定感染活性.收集制备假病毒过程中整合HA蛋白的293T细胞,通过流式细胞术测定2份恢复期血清中的HA抗体.利用2份恢复期血清分别与深圳株和泰国株假病毒进行中和试验,通过计算IC5o判断不同血清对2株假病毒中和效价的差异.结果 构建的2株假病毒颗粒不仅含有HIV基质蛋白P24,还可以整合HA蛋白,并且能够由前体蛋白HA0裂解为具有生物活性的HA1和HA2.200μL假病毒上清液感染靶细胞后RLA值约为2×104,具有较高的感染效价.FACS检测结果显示2份血清中均含有HA抗体,而且对不同分枝的假病毒具有交叉反应性.中和试验表明2份血清对深圳株和泰国株假病毒均具有中和能力,而且对不同分枝的假病毒具有交叉中和活性,但对深圳株的中和效价均高于泰国株.结论 本研究成功构建具有感染活性的深圳株和泰国株H5N1禽流感假病毒,并可以快速、安全、高效的评价血清的中和效价,具有广阔的应用前景.%Objective To establish neutralization assay based on H5N1 avian influenza pseudotyped virus in vitro and to evaluate neutralizing titer of convalescent serum from 2 patients with H5N1 avian influenza.Methods pHR-Luc,pCMV△8.2 and CMV/R-SH or CMV/R-TH were cotransfected into 293T cell by co-precipitation with calcium phosphate.Pseudotyped virus supernatant was harvested 72 h posttranofection and identified the expression of HA and P24 by Western blot,and then we analyzed infective activity of 200 μL supernatant of pseudotyped virus.293T cell

  6. Clinical and immunological efficacy of combination therapy of children with measles

    Directory of Open Access Journals (Sweden)

    V. N. Timchenko

    2016-01-01

    Full Text Available The aim of the study was to assess the clinical and immunological efficacy of recombinant interferon Alfa-2b. Examined 34 children aged from 1 year to 7 years carrying medium to heavy degree of measles. All patients were not vaccinated against measles. In the treatment of 18 people (Control group were used only basic therapy (pathogenic, symptomatic. At 16 people (Study group used a combination therapy (basic + Viferon. As an antiviral agent used and immunotropic preparation of human recombinant interferon alfa-2b in the form of rectal suppositories – Viferon (OOO «Feron», Russia. Immunological patient survey was conducted in the dynamics of the disease – during the height (in 1-6 days the rash and in convalescence period (10-16 days rash. Indicators of cellular immunity (leukocytes, lymphocytes, CD3 +, CD3 + CD (16 + 56 +, CD3 + CD4 +, CD3 + CD8 +, CD4 + CD8 +, CD3-CD8 +, CD3-CD (16 + 56, CD19 +, CD25 +, CD3 + HLA DR +, CD (16 + 56 + HLA DR +, HLA DR +, CD95 + were evaluated by flow cytometry. To assess the production of IFN-α and IFN-γ in the samples of peripheral blood were cultured in the CO2 incubator for 18 hours at 37 C and 4% CO2. The supernatants of cell culture were investigated with the purpose of studying spontaneous and induced production of interferon alpha and gamma. The concentrations of interferon α and γ (IFNα, IFNγ in the blood serum, as well as levels of spontaneous and induced cytokine production data of blood cells of sick children was determined by ELISA. All the children at the peak of the disease found a dramatic inhibition of cellular immunity and the production of cytokines, which is consistent with the concept of «measles anergy» accepted in the scientific literature. The period of convalescence oppression immunity indices remained, but it was less pronounced in the group of children treated with the drug of human recombinant interferon alfa-2b -Viferon. Clinical efficacy

  7. Dynamic changes of IL-2, sIL-2R, IL-6 and IL-8 in children with mycoplasma pneumonia and their clinical significance%肺炎支原体肺炎IL-2、sIL-2R、IL-6、IL-8检测

    Institute of Scientific and Technical Information of China (English)

    刘文彬; 赵文利; 汤雪琴; 袁丽

    2012-01-01

    目的 探讨肺炎支原体肺炎(MPP)急性期和恢复期免疫功能动态变化及临床意义.方法 检测IL-2、sIL-2R、IL-6、IL-8均采用ELISA双抗体夹心法.结果 与对照组比较,MPP患儿急性期sIL-2R、IL-6,IL-8值均明显升高(P<0.01),IL-2值均明显下降(P<0.01),与急性期比较,MPP患儿恢复期IL-2值均明显升高(P<0.01),而sIL-2R、IL-6、IL-8值均明显下降(P<0.01);与对照组比较,MPP患儿恢复期IL-2、sIL-2R、IL-6、IL-8均无明显改变(P>0.05).结论 MPP患儿存在细胞免疫功能低下及紊乱,IL-2、sIL-2R、IL-6及IL-8值恢复较快,检测MPP患儿免疫功能,特别是IL-2、sIL-2R、IL-6和IL-8水平对疗效及预后的判定有重要价值.%Objective To observe the dynamic changes of IL-2, sIL-2R, IL-6 and IL-8 in children with mycoplasma pneumonia( MPP) and to study their clinical significance. Methods The serum levels of IL-2, sIL-2R, IL-6 and IL-8 in 40 children with MPP during the acute stage and convalescent stage were examined with double-antibody sandwich enzyme-linked immu-nosobent assay, and the results were compared with those in 30 normal children. Results The levels of slL-2R, IL-6 and IL-8 were higher and IL-2 was lower during acute stage than those in the normal subjects (P <0. 01). Compared those during the convalescent stage, the level of IL-2 was higher and sIL-2R, IL-6 and IL-8 were lower (P <0. 01) during the acute stage. There were not any significant differences of the levels of these four substances between the normal children and MPP children during the convalescent stage. Conclusion The cellular immune function of children with MPP is impaired and the changes of IL-2, sIL-2R, IL-6 and IL-8 can be used to evaluate the curative effects and prognosis of MPP in children.

  8. Change and Clinical Significance of Serum IL-6, IL-10, and IL-17 level in Children with Hand, Foot, and Mouth Disease%手足口病患儿血清IL-6、IL-10、IL-17水平的变化及其临床意义

    Institute of Scientific and Technical Information of China (English)

    黄小霏; 檀卫平; 江润昌

    2012-01-01

    [Objective] To study the changes and clinical significance of serum interleukin (IL)-6, IL-10, and IL-17 levels in the children with hand foot and mouth disease (HFMD). [Method] The serum concentration of IL-6, IL-10, and IL-17 in 19 cases of HFMD children ( acute stage and convalescence ) and 20 cases of healthy controls were measured by ELJSA. The cytokines levels were compared. [Results] 1.Compared with healthy controls, the levels of IL-6, IL-10, and IL-17 in acute stage of HFMD were significantly increased in HFMD children (P 0.05 ), However the levels of IL-10 in convalescence of HFMD were higher than healthy children (P 0.05). [Conclusions] l.The levels of IL-6 and IL-17 were significantly increased in acute stage of HFMD children. 2. The persistent elevation of serum IL-10 level was found in HFMD patients after convalescence.%[目的]探讨手足口病(HFMD)患儿外周血白介素-6(IL-6)、白介素-10(IL-10)、白介素-17(IL-17)水平及其在HFMD发展过程中的变化.[方法]本实验采用酶联免疫吸附实验(ELISA)双抗体夹心法测定19例HFMD患儿(急性期、恢复期)及同期20例健康体检儿童血清IL-6、IL-10、IL-17的含量;并对HFMD患儿急性期、恢复期及健康儿童进行两两比较,了解IL-6、IL-10、IL-17在HFMD发展过程中的变化.[结果]1、手足口病患儿急性期IL-6、IL-10及IL-17水平显著高于健康对照组(P< 0.01);恢复期IL-6及IL-17水平与对照组比较无显著差异(P>0.05).而IL-10水平仍高于健康对照组(P<0.01).2、急性期IL-6与IL-10呈负相关关系(P< 0.05);IL-6与IL-17呈正相关关系(P< 0.01);IL-10与IL-17呈负相关关系(P<0.05).恢复期:IL-6与IL-10呈负相关关系(P< 0.05);IL-6与IL-17呈正相关关系(P<0.01);IL-10与IL-17无相关关系(P>0.05).[结论]手足口病患儿急性期炎症细胞因子( IL-6、IL-17)水平显著升高,而抑制性细胞因子IL-10水平升高持续到恢复期.

  9. 微创拔牙术在特勤疗养员下颌智齿拔除术中的临床应用

    Institute of Scientific and Technical Information of China (English)

    马才顺; 徐辉; 杨佳

    2015-01-01

    目的:观察微创拔牙技术在特勤疗养员下颌智齿拔除术中的临床应用效果。方法选择需拔除下颌智齿的特勤疗养员280例,随机分为两组,每组140例,即实验组(微创组)及对照组(传统组),分别用微创拔牙刀结合高速涡轮牙钻拔牙和传统拔牙器械拔除患牙,准确记录两种方法的手术时间、术后患者张口度、感染、肿胀、疼痛、满意度、干槽症等发生情况。结果运用微创拔牙刀结合高速涡轮牙钻拔牙与传统拔牙器械拔除患牙相比,拔牙时间明显缩短,差异具有高度统计学意义(P<0.01),术后患者张口度、感染、肿胀、疼痛、满意度、干槽症等发生率的差异具有统计学意义(P<0.05)。结论运用微创拔牙刀结合高速涡轮牙钻拔除特勤疗养员下颌智齿可达到无痛、安全、微创的效果;明显降低智齿拔除并发症。%Objective To observe the clinical application effect of minimally invasive exodontia technology on mandibular impacted tooth extraction among special-service convalescents.Methods 280 special-service convalescents needing mandibular impacted tooth extraction were randomly divided into two groups,the experimental group (minimal-ly invasive group )and the control group (traditional group )with 140 cases in each group ,who were respectively given minimally invasive exodontia combined with high-speed turbine tooth drill extraction and traditional dental extraction e-quipment extraction,and the operation time,post-operative mouth opening,infection,swelling,pain,satisfaction,and dry socket incidence of both methods were accurately recorded .Results C ompared with traditional dental extraction e-quipment extraction ,the method of minimally invasive exodontia combined with high-speed turbine tooth drill extrac-tion took clearly shorter tooth extraction time,and the differences were of high statistical significance (P<0 .01 ).The differences of post

  10. Analysis of clinical characteristics and plasma level of mannose-binding lectin in 68 children with febrile convulsion%68例热性惊厥患儿临床特点及其血浆甘露糖结合凝集素水平变化

    Institute of Scientific and Technical Information of China (English)

    王超前; 华春珍; 罗巧二; 王琦婧; 戴凯丽; 黄邢邢; 杨钦梳

    2012-01-01

    [Objective] To analyze the clinical characteristics of febrile convulsion (FC) occurred in children and study the mannose-binding lectin (MBL) levels in plasma at both acute and convalescent stages in the case group. [Methods] Plasma samples were separated from whole blood which was collected from children with FC at the acute and convalescent stages during August 2010 to July 2011. The MBL concentrations were measured by ELISA method and the SPSS 11. 0 software was used in the study. [Results] Among all 68 cases,The mean MBL levels were (595 + 559)ng/mL at acute stage and (585 ± 466)ng/mL in recovery stage,and no significant difference was found between the two groups (Z = 0. 207,P = 0. 836). However,there was a significant correlation of the MBL levels between the two stages (r=0. 809, P<0. 01). 29. 4% of all individuals had low MBL levels less than 200 ng/mL at convalescent stage. Acute reaction of MBL was observed in 38. 2% children with FC. No correlation was found between MBL levels and CRP levels(r =-0. 15,P=0. 22). It was found that 89. 7% of the FC children was caused by acute upper respiratory tract infection. The creatine kinase-MB exceeding normal value was found in 54. 1% of all individuals. [Conclusion] As a whole, convulsion acting as a kind of stress, had no significant effects on the MBL level in vivo.%[目的]了解热性惊厥患儿的临床特点及其血浆甘露糖结合凝集素(mannose-binding lectin,MBL)水平在惊厥前后的变化. [方法]对2010年8月-2011年7月在本院住院的68例热性惊厥患儿,应用ELISA法检测急性期与恢复期血浆MBL浓度,同时进行临床特点分析.统计分析采用SPSS 11.0. [结果]急性期和恢复期MBL平均浓度分别为(595±559) ng/mL和(585±466) ng/mL,二者差异无统计学意义(Z=0.207,P=0.836),急性期与恢复期MBL水平呈线性相关(r=0.809,P<0.01).其中恢复期MBL血浆浓度<200 ng/mL的病例占29.4%,MBL参与急性应答占全部病例的38.2%.MBL浓

  11. Application of virtual reality technique in rehabilitation of hemiplegic upper extremities function of stroke patients%虚拟现实技术在脑卒中患者偏瘫上肢功能康复中的应用

    Institute of Scientific and Technical Information of China (English)

    梁明; 窦祖林; 王清辉; 熊巍; 郑雅丹; 陈颖蓓; 杨琼; 邱雅贤

    2013-01-01

    目的:初步观察虚拟厨房上肢康复训练结合常规作业治疗对脑卒中恢复期患者偏瘫上肢功能康复的临床疗效.方法:将33例脑卒中恢复期偏瘫上肢功能障碍的患者随机分为治疗组(16例)和对照组(17例).对照组接受常规作业治疗每次40min,每日1次,每周5次,共3周.治疗组接受常规作业治疗和虚拟厨房上肢康复训练各20min,每次共40min,每日1次,每周5次,共3周.其余康复治疗如运动疗法和日常生活活动训练等两组均相同.两组患者分别于治疗前、治疗后予以FMA上肢部分(FMA-UE)、MAS上肢部分(MAS-UE)和MBI评定,比较两组的疗效.结果:两组患者治疗后FMA-UE、MAS-UE及MBI的评分均较治疗前提高,治疗前、后各量表的评分差异具有显著性(P< 0.05);与对照组相比,治疗组患者FMA-UE、MBI的评分提高幅度更大(P<0.05).结论:虚拟厨房上肢康复训练结合常规康复作业治疗能更好地改善脑卒中恢复期患者偏瘫上肢的运动功能,更有效地提高患者日常生活活动能力.%Objective: To observe the effect of virtual kitchen upper extremities training combined with traditional occupational therapy on hemiplegic upper extremities function of stroke patients in convalescent phase. Method: Thirty-three stroke patients with hemiplegic upper extremities dysfunction in convalescent phase, were divided into therapy group (n=16) and control group (n=17). The patients in control group accepted traditional occupational therapy, 40 min/d, 5d/week for 3 weeks. The patients in therapy group accepted virtual kitchen upper extremities training and traditional occupational therapy. Each part of virtual reality trainning lasted 20 min and the total was 40 min, the training schedule in therapy group was 40min/rl, 5d/week for 3 weeks. Physical therapy and training of activities of daily living were the same in both groups. Fugl-Meyer assessment of upper extremity (FMA-UE), motor assessment scale

  12. 综合疗养因子治疗飞行员下腰痛89例

    Institute of Scientific and Technical Information of China (English)

    熊万喜; 余琳; 王丽

    2013-01-01

      目的探讨采用综合疗养因子治疗飞行员下腰痛的方法与效果。方法采用自然疗养因子、人工理化因子,从健康教育入手,使飞行员了解和掌握下腰痛发生的病因病机和防治措施,以自然疗养因子疗法、运动疗法及心理疗法为重点,对89例下腰痛患者进行了治疗。结果对89例下腰痛患者进行严格、正规的综合康复治疗后,显效45例,占50.56%,好转36例,占40.45%,无效8例,占8.99%,总有效率为91.01%;其中腰肌劳损、腰肌筋膜炎疗效最好。结论通过对下腰痛进行完善、系统的综合康复治疗,不仅取得了较明显的效果,而且方法简便适宜,病员容易接受,既安全又不会引起其他功能紊乱,也没有药物治疗时可能产生的不良反应。%Objective To probe into the method and effect of using comprehensive convalescent factors to treat low back pain of pilots.Methods Natural convalescent factors and artificial physical and chemical factors were adopted to start with health education so that the pilots could understand and grasp the etiology and pathogenesis of low back pain as well as its control measures.89 sufferers with low back pain were treated with natural convalescent factors therapy,exercise therapy and psychotherapy.Results A fter strict and regular comprehensive rehabilitation ,45 had excellent effect accounting for 50 .56% ,36 had better improvement accounting for 40 .45% ,8 had no effect accounting for 8 .99% ,the total effective rate was 91 .01% ,among which lumbar muscle strain and lumbar fasciitis had best effects.Conclusion Through com plete and system atic comprehensive rehabilitation treatment on low back pain,clear effect has been achieved and this method is simple to be accepted by sufferers.It is safe without causing other dysfunction or untoward effect from drug therapy.

  13. Complement levels in Brazilian children during and after meningococcal meningitis Níveis de complemento em crianças brasileiras durante e após meningite meningocócica

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    Pérsio Roxo Júnior

    2005-04-01

    Full Text Available PURPOSE: To evaluate the functional activity of the classical and alternative pathways of the complement system and the levels of C3, C4, and factor B during the first episode of meningococcal infection and during the convalescence period. PATIENTS AND METHODS: Ten Brazilian children ranging in age from 8 months to 8 years, admitted from 1991 to 1993 with a clinical-laboratory diagnosis of meningococcal meningitis, were studied during acute infection (up to 7 days from diagnosis and during the convalescence period (1 to 6 months after the acute episode. C3, C4, and Factor B were measured using nephelometry, and the lytic activity of classical and alternative pathways were evaluated by a kinetic method and expressed as the time needed to lyse 50% of an erythrocyte suspension (T1/2, expressed in seconds. Low T1/2 values for classical and alternative pathways correlate with high activities of the classical and alternative complement pathways, respectively. RESULTS: A significant difference was observed between the alternative pathway lytic activity during infection and the convalescence period (282 vs 238 seconds, respectively, P = .01. No differences were detected in the other complement parameters analyzed. CONCLUSIONS: In the presence of meningococcal meningitis, the alternative pathway is preferentially activated. This is probably due to the greater ability of the meningococcal endotoxin to activate this pathway in vivo.OBJETIVO: Avaliar a atividade funcional das vias clássica e alternativa do sistema complemento e os níveis de C3, C4 e fator B durante o primeiro episódio de infecção meningocócica e durante a convalescença. PACIENTES E MÉTODOS: Dez crianças brasileiras com idades entre 8 meses e 8 anos, admitidas de 1991 a 1993, com diagnóstico clínico-laboratorial de meningite meningocócica, foram estudadas durante infecção aguda (até 7 dias do diagnóstico e no período de convalescença (entre 1 e 6 meses após. C3, C4 e fator

  14. 碳酸泉在康复疗养中的应用进展

    Institute of Scientific and Technical Information of China (English)

    韩麒智; 徐莉

    2015-01-01

    Objective To study and analyze the application and progress of natural convalescent factor of carbonate spring in rehabilitation and recuperation .Methods D om estic carbonate spring therapy and drinking therapy in recent years w as retrospectively analyzed in order to com prehensively assess their curative effects.Results C arbonate spring therapy and drinking therapy,as a rehabilitation treatm ent m ethod,show ed its advantages,w hose application to m edical and health field had proved its m edical effects. It w as w idely applied to the rehabilitation treatm ent of diseases such as angiocarpy,central nervous system ,skin,and stom ach and intestine.Conclusion N atural convalescent factor of car-bonate spring therapy should be em phasized and w idely prom oted in prevention,health care,and rehabilitation treat-m ent of diseases.%目的:研究分析自然疗养因子碳酸泉的应用与进展。方法回顾性文献分析近年国内碳酸泉浴疗、饮疗应用方法,综合评价评估其效果。结果碳酸泉浴疗、饮疗作为一种康复治疗方法,有其优势,通过碳酸泉浴疗、饮疗在保健医学领域中的应用,证实了碳酸泉疗法的医疗效果,并应用于心血管、中枢神经系统、皮肤、胃肠等疾病的康复治疗中。结论自然疗养因子碳酸泉疗法在应用中要受到重视,并在疾病的预防、保健和康复治疗中要广泛推广。

  15. Quantitative T2{sup *} assessment of knee joint cartilage after running a marathon

    Energy Technology Data Exchange (ETDEWEB)

    Hesper, Tobias [University Düsseldorf, Medical Faculty, Department of Orthopaedics, Düsseldorf (Germany); Miese, Falk R. [University Düsseldorf, Medical Faculty, Department of Diagnostic and Interventional Radiology, Düsseldorf (Germany); Hosalkar, Harish S. [Center of Hip Preservation and Children' s Orthopaedics, San Diego, CA (United States); Behringer, Michael [German Sport University, Cologne (Germany); Zilkens, Christoph [University Düsseldorf, Medical Faculty, Department of Orthopaedics, Düsseldorf (Germany); Antoch, Gerald [University Düsseldorf, Medical Faculty, Department of Diagnostic and Interventional Radiology, Düsseldorf (Germany); Krauspe, Rüdiger [University Düsseldorf, Medical Faculty, Department of Orthopaedics, Düsseldorf (Germany); Bittersohl, Bernd, E-mail: bbittersohl@partners.org [University Düsseldorf, Medical Faculty, Department of Orthopaedics, Düsseldorf (Germany)

    2015-02-15

    Highlights: • This is the first descriptive report on the effects of repetitive joint loading on the T2{sup **} relaxation assessment of articular cartilage. • This study on marathon runners who underwent MRI within 48 hours prior to and following the running event as well as after a period of convalescence of approximately four weeks confirms the feasibility of T2{sup **} mapping of knee joint cartilage under the consideration of repetitive joint loading prior to MRI as we noted only small differences in the T2{sup **} after running a marathon. • Despite the small study group (nn = 10) and the presence of morphologically normal appearing cartilage, we noted lower cartilage T2{sup **} values in the medial tibial plateau that may be related to functional demand or early signs of cartilage degeneration. - Abstract: Objective: To study the effect of repetitive joint loading on the T2{sup *} assessment of knee joint cartilage. Materials and methods: T2{sup *} mapping was performed in 10 non-professional marathon runners (mean age: 28.7 ± 3.97 years) with no morphologically evident cartilage damage within 48 h prior to and following the marathon and after a period of approximately four weeks. Bulk and zonal T2{sup *} values at the medial and lateral tibiofemoral compartment and the patellofemoral compartment were assessed by means of region of interest analysis. Pre- and post-marathon values were compared. Results: There was a small increase in the T2{sup *} after running the marathon (30.47 ± 5.16 ms versus 29.84 ± 4.97 ms, P < 0.05) while the T2{sup *} values before the marathon and those after the period of convalescence were similar (29.84 ± 4.97 ms versus 29.81 ± 5.17 ms, P = 0.855). Regional analyses revealed lower T2{sup *} values in the medial tibial plateau (P < 0.001). Conclusions: It appears that repetitive joint loading has a transient influence on the T2{sup *} values. However, this effect is small and probably not clinically relevant. The low T2

  16. Toscana virus meningo-encephalitis: an important differential diagnosis for elderly travellers returning from Mediterranean countries.

    Science.gov (United States)

    Veater, James; Mehedi, Farhan; Cheung, Chee Kay; Nabarro, Laura; Osborne, Jane; Wong, Nicholas; Wiselka, Martin; Tang, Julian W

    2017-08-29

    Elderly patients have a long list of differentials for causes of acute confusion and altered consciousness levels, including infectious agents. In addition, elderly, retired patients often have more time to travel for tourism, particularly to exotic, warmer locations. Mediterranean countries such as Spain and Italy are popular holiday destinations for British and other tourists, especially during the winter months. However, these warm climates allow insect vectors to proliferate, increasing the risk of exposure to endemic vectorborne viral infections whilst on vacation. Such infections may not be routinely considered by geriatric medical teams. An 87-year old gentleman presented with a three-day history of worsening confusion, lethargy, ataxia, and fevers following a trip to Spain, where he may have sustained a sandfly bite. By the time of admission, he had a reduced GCS, was hallucinating, and was incontinent of urine and faeces, though blood pressure and heart rate were normal. He also appeared hyperaesthetic, and found even capillary blood sugar testing extremely painful. He had no history of cognitive defect or other neurological conditions. He had been previously independently active, with frequent trips to Spain where he maintained a holiday home. He probably sustained a sandfly bite during this most recent trip, whilst cleaning out a shed. Acute and convalescent sera demonstrated IgG antibodies to Toscana virus at extremely high titres of ≥1:10,000 by immunofluorescence assay, though no Toscana virus RNA was detectable in these sera by the time of presentation. Toscana virus should be included in the differential diagnosis of any patients presenting with meningo-encephalitis who have recently returned from a Mediterranean country. Testing for Toscana virus infection is performed by serological testing on acute/convalescent paired sera, and/or a polymerase chain reaction (PCR) assay on blood or cerebrospinal fluid (CSF) if presenting within 5 days of

  17. 300例小儿支气管肺炎血小板参数的变化及临床意义%Alternation of platelet parameter in 300 children with bionchopneumonia

    Institute of Scientific and Technical Information of China (English)

    刘春艳; 贾鹏; 刘文君

    2012-01-01

    OBJECTIVE To investigate the alternation and clinical significance of platelet parameter in 300 children with bron-chopneumonia. METHODS Observed the alternation of 300 children patients with bronchopneumonia (severe pneumonia 130, mild pneumonia 170) platelet count (PLT), platelet hematocrit (PCT) , mean platelet volume (MPV), platelet distribution width (PDW), and compared it with 80 cases in normal control group. RESULTS The platelet parameters of severe pneumonia-group and mild pneumonia-group at acute stage were significantly higher than those of control group (P < 0.01); 4 platelet parameters in severe pneumonia group were significantly higher than mild pneumonia group at acute stage (P< 0.01); 4 platelet parameter in severe pneumonia梘roup and mild pneumonia group at convalescence stage were lower than those at acute stage (P < 0.01) and all indices came back to normal at convalescence stage. CONCLUSION The results of this study suggest that the 4 platelet parameters of children with bronchial pneumonia abnormally go up at acute stage the extent of rise is closely connected with the suite of an illness.%目的 探讨小儿支气管肺炎4项血小板参数的变化及意义.方法 观察300例小儿支气管肺炎(重症肺炎130例,轻症肺炎170例)患者的血小板计数(PLT)、血小板压积(PCT)、平均血小板体积(MPV)及血小板分布宽度(PDW)的变化,并与80例正常对照组比较.结果 重症肺炎组与轻症肺炎组急性期4项血小板参数比对照组均显著升高(P<0.01);重症肺炎组急性期4项血小板参数比轻症肺炎组急性期显著升高(P<0.01);重症肺炎组与轻症肺炎组恢复期4项血小板参数比急性期均显著降低(P<0.01),并恢复正常.结论 小儿支气管肺炎急性期4项血小板参数均异常升高,升高程度与病情相关.

  18. Differential expression of miR-145 in children with Kawasaki disease.

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    Chisato Shimizu

    Full Text Available BACKGROUND: Kawasaki disease is an acute, self-limited vasculitis of childhood that can result in structural damage to the coronary arteries. Previous studies have implicated the TGF-β pathway in disease pathogenesis and generation of myofibroblasts in the arterial wall. microRNAs are small non-coding RNAs that modulate gene expression at the post-transcriptional level and can be transported between cells in extracellular vesicles. To understand the role that microRNAs play in modifying gene expression in Kawasaki disease, we studied microRNAs from whole blood during the acute and convalescent stages of the illness. METHODOLOGY/PRINCIPAL FINDINGS: RNA isolated from the matched whole blood of 12 patients with acute and convalescent Kawasaki disease were analyzed by sequencing of small RNA. This analysis revealed six microRNAs (miRs-143, -199b-5p, -618, -223, -145 and -145* (complementary strand whose levels were significantly elevated during the acute phase of Kawasaki disease. The result was validated using targeted qRT-PCR using an independent cohort (n = 16. miR-145, which plays a critical role in the differentiation of neutrophils and vascular smooth muscle cells, was expressed at high levels in blood samples from acute Kawasaki disease but not adenovirus-infected control patients (p = 0.005. miR-145 was also detected in small extracellular vesicles isolated from acute Kawasaki disease plasma samples. Pathway analysis of the predicted targets of the 6 differentially expressed microRNAs identified the TGF-β pathway as the top pathway regulated by microRNAs in Kawasaki disease. CONCLUSION: Sequencing of small RNA species allowed discovery of microRNAs that may participate in Kawasaki disease pathogenesis. miR-145 may participate, along with other differentially expressed microRNAs, in regulating expression of genes in the TGF-β pathway during the acute illness. If the predicted target genes are confirmed, our findings suggest a model of

  19. Systemic T-helper and T-regulatory cell type cytokine responses in rhinovirus vs. respiratory syncytial virus induced early wheezing: an observational study

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    Vuorinen Tytti

    2009-09-01

    Full Text Available Abstract Background Rhinovirus (RV associated early wheezing has been recognized as an independent risk factor for asthma. The risk is more important than that associated with respiratory syncytial virus (RSV disease. No comparative data are available on the immune responses of these diseases. Objective To compare T-helper1 (Th1, Th2 and T-regulatory (Treg cell type cytokine responses between RV and RSV induced early wheezing. Methods Systemic Th1-type (interferon [IFN] -gamma, interleukin [IL] -2, IL-12, Th2-type (IL-4, IL-5, IL-13 and Treg-type (IL-10 cytokine responses were studied from acute and convalescence phase serum samples of sole RV (n = 23 and RSV affected hospitalized wheezing children (n = 27. The pre-defined inclusion criteria were age of 3-35 months and first or second wheezing episode. Analysis was adjusted for baseline differences. Asymptomatic children with comparable demographics (n = 11 served as controls for RV-group. Results RV-group was older and had more atopic characteristics than RSV-group. At acute phase, RV-group had higher (fold change IL-13 (39-fold, IL-12 (7.5-fold, IFN-gamma (6.0-fold and IL-5 (2.8-fold concentrations than RSV-group and higher IFN-gamma (27-fold, IL-2 (8.9-fold, IL-5 (5.6-fold and IL-10 (2.6-fold than the controls. 2-3 weeks later, RV-group had higher IFN-gamma (>100-fold, IL-13 (33-fold and IL-10 (6.5-fold concentrations than RSV-group and higher IFN-gamma (15-fold and IL-2 (9.4-fold than the controls. IL-10 levels were higher in acute phase compared to convalescence phase in both infections (p Conclusion Our results support a hypothesis that RV is likely to trigger wheezing mainly in children with a predisposition. IL-10 may have important regulatory function in acute viral wheeze.

  20. Effect of thyroid hormone on serum leptin levels in critically ill patients with NTIS%甲状腺激素对 NTIS 患者血清瘦素的影响

    Institute of Scientific and Technical Information of China (English)

    林巧; 时立新

    2014-01-01

    研究甲状腺激素(TH)对合并非甲状腺病态综合征(NTIS)的危重症患者血清瘦素(Leptin)的影响。方法:1.比较危重症组和正常对照组的 TH 及Leptin的水平。2.比较存活的 NTIS患者在急性期和缓解期的血清 TH 及Leptin水平的变化。3.比较死亡组、未愈组和好转组患者的血清 TH及Leptin水平的变化。结果:1.危重症组血清FT3、FT4、TSH 水平均明显低于正常对照组,而血清 Leptin 高于对照组(P<0.05)。2.缓解期患者血清FT3、FT4、TSH均高于急性期,而血清 Leptin 低于急性期(P<0.05)。3.死亡组、未愈组患者血清 FT3、FT4均低于好转组,有统计学差异(P<0.05);而血清 Leptin均低于好转组,无统计学差异(P>0.05)。结论:在合并 NTIS危重症患者中 TH 可能参与血清Leptin的调节。%To investigate the effect of thyroid hormone on serum leptin levels in critically ill patients with non - thyroid sick syndrome (NTIS) .Methods :1 .The serum thyroid hormone(FT3 ,FT4 ,TSH) and leptin levels were measured in non -thyroidal patients and compared :(1) between critical illness and control group .(2) between acute stage and convalescence in survivors .(3) between death ,non -recovery and convalescence of NTIS pa‐tients .Results :1 .Compared with control group ,the serum FT3 ,FT4 ,TSH in critical patients were dramatically lower (P0 .05) .Conclusions :TH may be involved in regulation of leptin in critically ill patients with NTIS .

  1. Analysis of HBV-specific cytotoxic T lymphocytes in patients with hepatitis B

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    Ning DING

    2011-04-01

    Full Text Available Objective To investigate the expression feature of hepatitis B virus(HBV-specific cytotoxic T lymphocytes(CTL,and analyze the role of CTL in immune response.Methods Peripheral venous blood was collected from 40 acute hepatitis B(AHB patients hospitalized from Oct.2008 to Jun.2010 in 302 Hospital of PLA for the detection of human leucocyte antigen-A2(HLA-A2.Out of the 40 patients,18 were HLA-A2-positive,and their peripheral blood mononuclear cells were isolated at acute phase and convalescent phase if the patients could be contacted.Six HLA-A2-negative AHB patients,18 HLA-A2-positive chronic HB patients and 6 HLA-A2-positive normal persons served as controls.The CTL was stained by fluorescence-labeled anti-CD3 monoclonal antibody,anti-CD8 monoclonal antibody and HBV epitopic pentamers(S183-191,S335-343,and examined with flow cytometry,then analyzed by software Cell-quest.The peak value of alanine aminotransferase(ALT and HBV load was detected at acute phase of patients,and the correlation was analyzed.Results The average frequencies were 0.23%±0.18% for HBsAg183-191-specific CTL and 0.49%±0.31% for HBsAg335-343-specific CTL at the acute phase of the 18 AHB patients,and they were significantly higher than those in patients with chronic HB,in whom CTL frequencies were 0.07%±0.03% for HBsAg183-191-specific CTL and 0.08%±0.04% for HBsAg335-343-specific CTL(P < 0.01.There was no correlation between the frequency of HBsAg183-191-specific CTL and the peak value of ALT(r=0.4506,P=0.0528,while the frequency of HBsAg335-343-specific CTL was correlated with ALT level(r=0.5642,P=0.0119.Neither the two CTL frequencies showed correlation with virus load.Compared with the acute phase,the CTL frequencies obviously decreased at convalescent phase(P=0.011.Both kinds of CTL were not detectable in HLA-A2-negative AHB patients and HLA-A2-positive healthy individuals.Conclusion HBV-specific CTL may play an important role in both virus clearance and liver

  2. Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue fever.

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    Luzia Maria de-Oliveira-Pinto

    Full Text Available Little is known about the role of chemokines/chemokines receptors on T cells in natural DENV infection. Patients from DENV-2 and -3- outbreaks were studied prospectively during the acute or convalescent phases. Expression of chemokine receptor and activation markers on lymphocyte subpopulations were determined by flow cytometry analysis, plasma chemokine ligands concentrations were measured by ELISA and quantification of CCL5/RANTES(+ cells in liver tissues from fatal dengue cases was performed by immunochemistry. In the acute DENV-infection, T-helper/T-cytotoxic type-1 cell (Th1/Tc1-related CCR5 is significantly higher expressed on both CD4 and CD8 T cells. The Th1-related CXCR3 is up-regulated among CD4 T cells and Tc2-related CCR4 is up-regulated among CD8 T cells. In the convalescent phase, all chemokine receptor or chemokine ligand expression tends to reestablish control healthy levels. Increased CCL2/MCP-1 and CCL4/MIP-1β but decreased CCL5/RANTES levels were observed in DENV-patients during acute infection. Moreover, we showed an increased CD107a expression on CCR5 or CXCR3-expressing T cells and higher expression of CD29, CD44(HIGH and CD127(LOW markers on CCR4-expressing CD8 T cells in DENV-patients when compared to controls. Finally, liver from dengue fatal patients showed increased number of cells expressing CCL5/RANTES in three out of four cases compared to three death from a non-dengue patient. In conclusion, both Th1-related CCR5 and CXCR3 among CD4 T cells have a potential ability to exert cytotoxicity function. Moreover, Tc1-related CCR5 and Tc2-related CCR4 among CD8 T cells have a potential ability to exert effector function and migration based on cell markers evaluated. The CCR5 expression would be promoting an enhanced T cell recruitment into liver, a hypothesis that is corroborated by the CCL5/RANTES increase detected in hepatic tissue from dengue fatal cases. The balance between protective and pathogenic immune response

  3. Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue fever.

    Science.gov (United States)

    de-Oliveira-Pinto, Luzia Maria; Marinho, Cíntia Ferreira; Povoa, Tiago Fajardo; de Azeredo, Elzinandes Leal; de Souza, Luiza Assed; Barbosa, Luiza Damian Ribeiro; Motta-Castro, Ana Rita C; Alves, Ada M B; Ávila, Carlos André Lins; de Souza, Luiz José; da Cunha, Rivaldo Venâncio; Damasco, Paulo Vieira; Paes, Marciano Viana; Kubelka, Claire Fernandes

    2012-01-01

    Little is known about the role of chemokines/chemokines receptors on T cells in natural DENV infection. Patients from DENV-2 and -3- outbreaks were studied prospectively during the acute or convalescent phases. Expression of chemokine receptor and activation markers on lymphocyte subpopulations were determined by flow cytometry analysis, plasma chemokine ligands concentrations were measured by ELISA and quantification of CCL5/RANTES(+) cells in liver tissues from fatal dengue cases was performed by immunochemistry. In the acute DENV-infection, T-helper/T-cytotoxic type-1 cell (Th1/Tc1)-related CCR5 is significantly higher expressed on both CD4 and CD8 T cells. The Th1-related CXCR3 is up-regulated among CD4 T cells and Tc2-related CCR4 is up-regulated among CD8 T cells. In the convalescent phase, all chemokine receptor or chemokine ligand expression tends to reestablish control healthy levels. Increased CCL2/MCP-1 and CCL4/MIP-1β but decreased CCL5/RANTES levels were observed in DENV-patients during acute infection. Moreover, we showed an increased CD107a expression on CCR5 or CXCR3-expressing T cells and higher expression of CD29, CD44(HIGH) and CD127(LOW) markers on CCR4-expressing CD8 T cells in DENV-patients when compared to controls. Finally, liver from dengue fatal patients showed increased number of cells expressing CCL5/RANTES in three out of four cases compared to three death from a non-dengue patient. In conclusion, both Th1-related CCR5 and CXCR3 among CD4 T cells have a potential ability to exert cytotoxicity function. Moreover, Tc1-related CCR5 and Tc2-related CCR4 among CD8 T cells have a potential ability to exert effector function and migration based on cell markers evaluated. The CCR5 expression would be promoting an enhanced T cell recruitment into liver, a hypothesis that is corroborated by the CCL5/RANTES increase detected in hepatic tissue from dengue fatal cases. The balance between protective and pathogenic immune response mediated by

  4. Vigilancia del dengue basada en el laboratorio: diferencias en el número de casos y virus aislados según la recolección del suero y la prueba serológica

    Directory of Open Access Journals (Sweden)

    Luis Ángel Villar

    2005-06-01

    Full Text Available Background: Dengue control measures depend on the laboratory-based surveillance data. In this study was investigated the differences in dengue cases and viruses isolated in according with surveillance strategy. Study design and methods: Two surveillance strategies were analyzed in this study. First (Local Surveillance System: single serum sample (acute or convalescent was collected from 337 dengue suspected cases and then kept at 4oC. IgM antibodies were detected using UMELISA DENGUE kit (IPK, Cuba, and serotypes identification was performed by viral isolation attempts in C6/36. Second: paired serum sample were collected from 318 dengue suspected cases and then kept at -70 ºC. IgM antibodies were detected using MAC-ELISA test and virus isolation as above. Some paired sera analyzed by MAC-ELISA were processed by “Dengue IgM-capture ELISA” kit (PANBIO and “IgM*ELISA anti-dengue” (IPK, Cuba. Results: More IgM+ dengue cases were detected by the first than second strategy using acute or convalescent serum: 61.1 vs 22% (p<0.001 y 86.8 vs 49.3% (p<0.001, respectively. Dengue diagnosis by MAC-ELISA was concordant in 85% (K=0.29 respect to UM-ELISA, but 100% (K=1 and 90.4% (K=0.84 respect to “IgM ELISA anti-dengue” and “Dengue IgM-capture ELISA” kits. Differences in dengue cases detected by the second strategy were found when serological result with single acute or paired samples was compared: 70 (22% vs 155 (48.7% (p<0.001. Dengue virus was isolated more frequently from sera kept at -70 ºC than those kept at 4 ºC: 17.8% vs. 4.7% (p<0.001. All virus serotypes were isolated. The UMELISA DENGUE test specificity and relationship between impact dengue surveillance and implemented strategy are analyzed. Conclusions: The specificity of UMELISA DENGUE test require to be evaluated by the high frequency of dengue in suspected cases. Serum paired analyzes in dengue surveillance is necessary to obtain reliable information.

  5. Probing vaccine antigens against bovine mastitis caused by Streptococcus uberis.

    Science.gov (United States)

    Collado, Rosa; Prenafeta, Antoni; González-González, Luis; Pérez-Pons, Josep Antoni; Sitjà, Marta

    2016-07-19

    Streptococcus uberis is a worldwide pathogen that causes intramammary infections in dairy cattle. Because virulence factors determining the pathogenicity of S. uberis have not been clearly identified so far, a commercial vaccine is not yet available. Different S. uberis strains have the ability to form biofilm in vitro, although the association of this kind of growth with the development of mastitis is unknown. The objective of this study was to evaluate the potential use as vaccine antigens of proteins from S. uberis biofilms, previously identified by proteomic and immunological analyses. The capability of eliciting a protective immune response by targeted candidates was assayed on a murine model. Sera from rabbits immunized with S. uberis biofilm preparations and a convalescent cow intra-mammary infected with S. uberis were probed against cell wall proteins from biofilm and planktonic cells previously separated by two-dimensional gel electrophoresis. Using rabbit immunized serum, two proteins were found to be up-regulated in biofilm cells as compared to planktonic cells; when serum from the convalescent cow was used, up to sixteen biofilm proteins were detected. From these proteins, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), fructose-biphosphate aldolase (FBA), and elongation factor Ts (EFTs) were chosen to be tested as vaccine antigen candidates. For this purpose, different groups of mice were immunized with the three recombinant-expressed proteins (each one formulated separately in a vaccine), and thereafter intraperitoneally challenged with S. uberis. The three proteins induced specific IgG antibodies, but a significant reduction of mortality was only observed in the groups of mice vaccinated with FBA or EFTs. These results suggest that FBA and EFTs might be considered as strong antigenic candidates for a vaccine against S. uberis bovine mastitis. Moreover, this is the first study to indicate that also in S. uberis, GAPDH, FBA and EFTs, as proteins

  6. Serum vitamin B12 and folic Acid levels in acute cerebral atherothrombotic infarction.

    Science.gov (United States)

    Kocer, Abdulkadir; Ince, Nurhan; Canbulat, Cuneyt E; Sargin, Mehmet

    2004-10-01

    Hyperhomocysteinemia is an independent risk factor for atherothrombotic cerebral stroke. Vitamin B12 and folic acid are important determinants of homocysteine metabolism. We aimed to evaluate the relationship, if present, between vitamin B12 and folic acid levels and acute cerebral stroke in this study. Blood aliquots drawn within 24 hours after the stroke from hospitalized patients (n=66) with the diagnosis of acute ischemic cerebrovascular episode and also blood samples from 38 healthy controls without any vascular risk factor were analyzed. With a competitive, chemoluminescence assay, serum levels of vitamin B12 and folic acid were measured in blood samples taken within 24 hours after the stroke. The differences and correlations were tested using frequency test, student-t test and multivariate analysis. Mean serum vitamin B12 levels were significantly lower in the patients than in the control subjects, 245.40 (S.D.: 72.9) and 343.2 (S.D.: 113.0) pg/ml respectively (p=0.0001). This difference was independent from other risk factors. Likewise, mean serum folic acid levels were lower in the patients than in the control subjects, 4.62 (S.D.: 1.94) and 5.97 (S.D.: 1.19) ng/ml, respectively (p=0.003). Mean serum levels of vitamin B12 and folate at the convalescence phase were 253.05 (S.D.: 68.78) pg/ml and 4.48 (S.D.: 2.08) ng/ml, respectively; the values obtained at the acute phase were not significantly different from the values obtained at the convalescence phase. We conclude that low vitamin B12 and folic acid concentrations are associated with an increased risk of stroke, and the relationship for vitamin B12 is independent from the other known modifiable stroke risk factors. For understanding the effects of B12 and folate in stroke patients, more detailed follow-up studies with long period are needed.

  7. MOTOmed智能运动训练系统训练对脑卒中偏瘫患者平衡功能及日常生活活动能力的影响%The effect of MOTOmed movement therapy on balance and ability in the activities of daily living in patients with hemiplegia after stroke

    Institute of Scientific and Technical Information of China (English)

    陈冲; 高晓平; 冯小军

    2010-01-01

    目的 探讨MOTOmed智能运动训练系统训练对脑卒中偏瘫患者平衡功能及日常生活活动(ADL)能力的影响.方法 将40例脑卒中患者按随机数法随机分为治疗组和对照组,每组20例.对照组患者采用常规康复训练方法 ,治疗组在此基础上,增加MOTOmed智能运动训练系统训练.治疗前和治疗6周后分别采用Berg平衡量表与Barthel指数(BI)评定患者的平衡功能和ADL能力.结果 治疗后2组患者的平衡功能和ADL能力均有明显改善(P<0.05),治疗组效果优于对照组(P<0.05).平衡功能与ADL呈正相关.结论 MOTOmed训练系统训练配合常规康复训练可较好的改善恢复期脑卒中偏瘫患者的平衡功能和日常生活活动能力.%Objective To investigate the effect of MOTOmed movement therapy on balance and ability in the activities of daily living (ADL) of convalescing stroke patients with hemiplegia. Methods Forty convalescent stroke patients exhibiting hemiplegia were randomly divided into a treatment group and a control group with 20 cases in each group. The control group received routine rehabilitation training; the treatment group received MOTOmed training in addition. Balance function of all the patients was assessed using Berg's balance scale (BBS) , and the Barthel Index ( BI) was used to assess ADL ability at the beginning of the program and after 6 weeks of treatment. Results Balance and ADL ability improved significantly in both groups after 6 weeks of treatment. The effect in the treatment group was significantly better than in the control group. Balance and ADL ability were positively correlated. Conclusions Applying MOTOmed therapy along with routine rehabilitation training can distinctly improve balance and the ADL ability of hemiplegics after stroke.

  8. Racing performance of Thoroughbreds with superficial digital flexor tendonitis treated with desmotomy of the accessory ligament of the superficial digital flexor tendon: 332 cases (1989-2003).

    Science.gov (United States)

    Hu, Alaine J; Bramlage, Larry R

    2014-06-15

    To assess postoperative probability of racing, career longevity, and convalescent time in Thoroughbred racehorses with moderate to severe superficial digital flexor tendonitis (SDFT) in the forelimbs treated by desmotomy of the accessory ligament of the superficial digital flexor tendon (ie, superior check ligament desmotomy [SCLD]). Retrospective case series. 332 Thoroughbred racehorses with SDFT consecutively treated by means of SCLD. Medical records and racing records were reviewed to assess return to racing, number of races completed, time to first race, and lifetime performance. The horses were categorized as raced or unraced prior to and after surgery. Descriptive statistics including age and treated limb were also recorded. Of 332 horses, 228 (69%) returned to racing following injury and treatment. Seventy-eight of 118 (66%) horses that had not raced prior to injury and 150 of 214 (70%) horses that had raced prior to injury raced after treatment. Seventeen of 39 (44%) horses ≥ 5 years old raced following injury and treatment and 211 of 293 (72%) horses ≤ 4 years old returned to racing. There was no difference in the percentages of horses returning to racing for 2-, 3-, or 4-year olds. Postoperative infections occurred in 6 of the 332 (2%) horses. Median time to first race for horses that raced after surgery was 302 days (range, 48 to 1,120 days; mean ± SD, 341 ± 153 days), with a median of 8 starts/horse after surgery (range, 1 to 109 starts; mean ± SD, 14 ± 15.8 starts). Of 228 horses that returned to racing, 159 (70%) raced ≥ 5 times after surgery. Sex and treated limb did not have a significant effect on return to racing. However, horses ≥ 5 years old were significantly less likely to return to racing, compared with younger horses. In horses with unilateral SDFT and racing was not significantly different between horses treated bilaterally versus unilaterally. 228 of 332 (69%) horses with SDFT of the forelimb treated with SCLD successfully

  9. Investigation of 222 Rn Released from Water and Dose Estimation at Hot Springs in Xianning City%咸宁温泉场所水氡释放调查及剂量评价

    Institute of Scientific and Technical Information of China (English)

    刘伟富; 廖海涛; 李晓芸; 姜子英; 黄志军; 李金凤

    2015-01-01

    本工作分析测量了咸宁市某温泉场所水中氡和室内外空气氡及其子体浓度,并对现场工作人员和疗养人员进行了剂量评价。温泉池在注水过程中,水中氡释放量在41~77%之间,室内温泉注水后空气中氡浓度会增加约25~30%,室外温泉池周围空气氡为环境对照点1.6~2.0倍,室内外氡及其子体平衡因子分别为0.54和0.67。剂量评价表明温泉场所工作人员所受氡的剂量高于湖北省居民的平均水平,疗养人员的受照剂量则与居民基本相当。%This study measured the concentrations of waterborne radon and airborne radon/radon progenies at hot springs in Xianning City, and estimated the annual effective doses of staff and convalescent people due to the airborne radon and its progenies.In the process of water injection, the transfer rate of radon from water to air varied from 41%to 77%.The concentrations of indoor radon were increased by 25~30% after water injec-tion.The concentrations of outdoor radon around the hot spring pools were 1.6 ~2 times of those at the envi-ronmental control point.The equilibrium factors were 0.54 and 0.67 for indoor and outdoor radon with their progenies respectively.Dose estimation showed that the annual effective dose of staff was higher than that of res-idents in Hubei Province, while the annual effective dose of convalescent people were equivalent to that of resi-dents.

  10. Host response transcriptional profiling reveals extracellular components and ABC (ATP-binding cassette transporters gene enrichment in typhoid fever-infected Nigerian children

    Directory of Open Access Journals (Sweden)

    Resau James H

    2011-09-01

    Full Text Available Abstract Background Salmonella enterica serovar Typhi (S. Typhi is a human-specific pathogen that causes typhoid fever, and remains a global health problem especially in developing countries. Its pathogenesis is complex and host response is poorly understood. In Africa, typhoid fever can be a major cause of morbidity in young infected children. The onset of the illness is insidious and clinical diagnosis is often unreliable. Gold standard blood culture diagnostic services are limited, thus rapid, sensitive, and affordable diagnostic test is essential in poor-resourced clinical settings. Routine typhoid fever vaccination is highly recommended but currently licensed vaccines provide only 55-75% protection. Recent epidemiological studies also show the rapid emergence of multi-drug resistant S. Typhi strains. High-throughput molecular technologies, such as microarrays, can dissect the molecular mechanisms of host responses which are S. Typhi-specific to provide a comprehensive genomic component of immunological responses and suggest new insights for diagnosis and treatment. Methods Global transcriptional profiles of S. Typhi-infected young Nigerian children were obtained from their peripheral blood and compared with that of other bacteremic infections using Agilent gene expression microarrays. The host-response profiles of the same patients in acute vs. convalescent phases were also determined. The top 96-100 differentially-expressed genes were identified and four genes were validated by quantitative real-time PCR. Gene clusters were obtained and functional pathways were predicted by DAVID (Database for Annotation, Visualization and Integrated Discovery. Results Transcriptional profiles from S. Typhi-infected children could be distinguished from those of other bacteremic infections. Enriched gene clusters included genes associated with extracellular peptides/components such as lipocalin (LCN2 and systemic immune response which is atypical in

  11. Effects of swimming training in thermal spring on pilots' pulmonary function%温泉游泳训练对飞行员肺功能影响的研究

    Institute of Scientific and Technical Information of China (English)

    王忠波

    2011-01-01

    Objective; To observe the effects of swimming training in thermal spring on pilots'pulmonary function. Methods; Twenty four healthy male pilots in active service were selected as subjects. During the period of convalescence, they were arranged to perform a four - week swimming training program (700 m swimming training in thermal spring every day, 5 days/week ). The changes of their pulmonary function before and after training were measured with pulmonary function testing equipment, respectively. Results; After four - week swimming training in thermal water, pilots'FVC(L), IVC(L), MVV(L/S), VC(L), PEF(L/S) increased significantly (P0.05). Conclusion; During the convalescence, swimming training in thermal spring can increase pilots'pulmonary function. However, the training has little effect on improving the small airway function.%目的:观察健康疗养期间温泉游泳训练对飞行员肺功能指标的影响.方法:24名现役飞行员在健康疗养期间,进行每周5d、连续4周的温泉游泳训练.训练前后采用采用肺功能检测仪检测肺功能指标.结果:与训练前相比,游泳训练4周后飞行员用力肺活量(FVC)、最大自然换气量(MVV)、呼出峰值流量(PEF)、慢吸气肺活量(IVC)、慢呼出肺活量(VC)、训练前后均有显著变化(P<0.01);小气道功能训练前后没有显著变化.结论:健康疗养期间,温泉游泳锻炼可提高肺活量,但对小气道功能的改变不明显.

  12. Extra-cellular expansion in the normal, non-infarcted myocardium is associated with worsening of regional myocardial function after acute myocardial infarction.

    Science.gov (United States)

    Garg, Pankaj; Broadbent, David A; Swoboda, Peter P; Foley, James R J; Fent, Graham J; Musa, Tarique A; Ripley, David P; Erhayiem, Bara; Dobson, Laura E; McDiarmid, Adam K; Haaf, Philip; Kidambi, Ananth; Crandon, Saul; Chew, Pei G; van der Geest, R J; Greenwood, John P; Plein, Sven

    2017-09-25

    Expansion of the myocardial extracellular volume (ECV) is a surrogate measure of focal/diffuse fibrosis and is an independent marker of prognosis in chronic heart disease. Changes in ECV may also occur after myocardial infarction, acutely because of oedema and in convalescence as part of ventricular remodelling. The objective of this study was to investigate changes in the pattern of distribution of regional (normal, infarcted and oedematous segments) and global left ventricular (LV) ECV using semi-automated methods early and late after reperfused ST-elevation myocardial infarction (STEMI). Fifty patients underwent cardiovascular magnetic resonance (CMR) imaging acutely (24 h-72 h) and at convalescence (3 months). The CMR protocol included: cines, T2-weighted (T2 W) imaging, pre-/post-contrast T1-maps and LGE-imaging. Using T2 W and LGE imaging on acute scans, 16-segments of the LV were categorised as normal, oedema and infarct. 800 segments (16 per-patient) were analysed for changes in ECV and wall thickening (WT). From the acute studies, 325 (40.6%) segments were classified as normal, 246 (30.8%) segments as oedema and 229 (28.6%) segments as infarct. Segmental change in ECV between acute and follow-up studies (Δ ECV) was significantly different for normal, oedema and infarct segments (0.8 ± 6.5%, -1.78 ± 9%, -2.9 ± 10.9%, respectively; P < 0.001). Normal segments which demonstrated deterioration in wall thickening at follow-up showed significantly increased Δ ECV compared with normal segments with preserved wall thickening at follow up (1.82 ± 6.05% versus -0.10 ± 6.88%, P < 0.05). Following reperfused STEMI, normal myocardium demonstrates subtle expansion of the extracellular volume at 3-month follow up. Segmental ECV expansion of normal myocardium is associated with worsening of contractile function.

  13. Antiviral Biologic Produced in DNA Vaccine/Goose Platform Protects Hamsters Against Hantavirus Pulmonary Syndrome When Administered Post-exposure

    Science.gov (United States)

    Henderson, Thomas; Nilles, Matthew L.; Kwilas, Steve A.; Josleyn, Matthew D.; Hammerbeck, Christopher D.; Schiltz, James; Royals, Michael; Ballantyne, John; Hooper, Jay W.; Bradley, David S.

    2015-01-01

    Andes virus (ANDV) and ANDV-like viruses are responsible for most hantavirus pulmonary syndrome (HPS) cases in South America. Recent studies in Chile indicate that passive transfer of convalescent human plasma shows promise as a possible treatment for HPS. Unfortunately, availability of convalescent plasma from survivors of this lethal disease is very limited. We are interested in exploring the concept of using DNA vaccine technology to produce antiviral biologics, including polyclonal neutralizing antibodies for use in humans. Geese produce IgY and an alternatively spliced form, IgYΔFc, that can be purified at high concentrations from egg yolks. IgY lacks the properties of mammalian Fc that make antibodies produced in horses, sheep, and rabbits reactogenic in humans. Geese were vaccinated with an ANDV DNA vaccine encoding the virus envelope glycoproteins. All geese developed high-titer neutralizing antibodies after the second vaccination, and maintained high-levels of neutralizing antibodies as measured by a pseudovirion neutralization assay (PsVNA) for over 1 year. A booster vaccination resulted in extraordinarily high levels of neutralizing antibodies (i.e., PsVNA80 titers >100,000). Analysis of IgY and IgYΔFc by epitope mapping show these antibodies to be highly reactive to specific amino acid sequences of ANDV envelope glycoproteins. We examined the protective efficacy of the goose-derived antibody in the hamster model of lethal HPS. α-ANDV immune sera, or IgY/IgYΔFc purified from eggs, were passively transferred to hamsters subcutaneously starting 5 days after an IM challenge with ANDV (25 LD50). Both immune sera, and egg-derived purified IgY/IgYΔFc, protected 8 of 8 and 7 of 8 hamsters, respectively. In contrast, all hamsters receiving IgY/IgYΔFc purified from normal geese (n=8), or no-treatment (n=8), developed lethal HPS. These findings demonstrate that the DNA vaccine/goose platform can be used to produce a candidate antiviral biological product

  14. 颧脂肪垫悬吊技术辅以SMAS折叠的中面部除皱术%Midface lifting of the suspension of the malar fat pad with SMAS plication

    Institute of Scientific and Technical Information of China (English)

    张志宏; 李文志; 欧阳钟石; 王佳琦

    2011-01-01

    目的:出于中面部提升简单化的趋势,介绍一种颧脂肪垫提升和SMAS移位的除皱技术.以期改善颧下区域空虚、鼻唇沟加深等中面部老化的问题.方法:采用一种中面部年轻化方法,行颊部耳前SMAS折叠,同时将下垂的颧脂垫缝线悬吊于耳前筋膜,使老化中面部维持长期的提升效果.结果:2006~2010年,在52例面部除皱术中运用该技术,中面部提升效果维持时间长,恢复期短,无面神经损伤等严重并发症出现.结论:皮下层的安全剥离避免了面神经分支的损伤.颧脂肪垫悬吊技术辅以SMAS折叠术,避免了较长的恢复期和深层剥离可能导致的损伤.这种方法可作为独立的中面部手术在局麻下实施,操作简单、安全、效果持久.%Objective A trend toward simplification in midface lifting has introduced the technique of suspension of the malar fat pad with SMAS plication. The questions of midfacial aging including hollowing in the submalar area, deepening of the nasolabial crease, can be correctted by this technique. Methods We describe an approach to midfacial rejuvenation that combines suture suspension of the ptotic malar fat pad to the preauricular fascia with baccul SMAS placation. Long-lasting improvement of the aging midface is achieved. Results This technique was used in 52 patients between 2006 and 2010, producing effective long-lasting results with limited convalescence and minimal complication rates. Conclusions Safe dissection in the subcutaneous plane avoids injury to facial nerve branches. Plication of the SMAS with suture suspension of the malar fat pad avoids the prolonged convalescence and other morbidities of deep plane dissections. This approach can be quickly and easily performed under local anesthesia as an isolated midface procedure, and it is a simple and safe procedure with effective long-lasting results.

  15. Antiviral Biologic Produced in DNA Vaccine/Goose Platform Protects Hamsters Against Hantavirus Pulmonary Syndrome When Administered Post-exposure.

    Directory of Open Access Journals (Sweden)

    Nicole Haese

    Full Text Available Andes virus (ANDV and ANDV-like viruses are responsible for most hantavirus pulmonary syndrome (HPS cases in South America. Recent studies in Chile indicate that passive transfer of convalescent human plasma shows promise as a possible treatment for HPS. Unfortunately, availability of convalescent plasma from survivors of this lethal disease is very limited. We are interested in exploring the concept of using DNA vaccine technology to produce antiviral biologics, including polyclonal neutralizing antibodies for use in humans. Geese produce IgY and an alternatively spliced form, IgYΔFc, that can be purified at high concentrations from egg yolks. IgY lacks the properties of mammalian Fc that make antibodies produced in horses, sheep, and rabbits reactogenic in humans. Geese were vaccinated with an ANDV DNA vaccine encoding the virus envelope glycoproteins. All geese developed high-titer neutralizing antibodies after the second vaccination, and maintained high-levels of neutralizing antibodies as measured by a pseudovirion neutralization assay (PsVNA for over 1 year. A booster vaccination resulted in extraordinarily high levels of neutralizing antibodies (i.e., PsVNA80 titers >100,000. Analysis of IgY and IgYΔFc by epitope mapping show these antibodies to be highly reactive to specific amino acid sequences of ANDV envelope glycoproteins. We examined the protective efficacy of the goose-derived antibody in the hamster model of lethal HPS. α-ANDV immune sera, or IgY/IgYΔFc purified from eggs, were passively transferred to hamsters subcutaneously starting 5 days after an IM challenge with ANDV (25 LD50. Both immune sera, and egg-derived purified IgY/IgYΔFc, protected 8 of 8 and 7 of 8 hamsters, respectively. In contrast, all hamsters receiving IgY/IgYΔFc purified from normal geese (n=8, or no-treatment (n=8, developed lethal HPS. These findings demonstrate that the DNA vaccine/goose platform can be used to produce a candidate antiviral

  16. First findings on the seroepidemiology of human paragonimosis at the anti-tuberculosis centre of Divo, Republic of Ivory Coast (West Africa

    Directory of Open Access Journals (Sweden)

    Aka N.A.

    2008-06-01

    Full Text Available An epidemiological study was carried out in 2004-2005 at the anti-tuberculosis centre of Divo (Ivory Coast to collect sera from patients who consulted for tuberculosis suspicion and to estimate the seroprevalence of human paragonimosis in the context of a systematic screening. No Paragonimus egg was found in the stools and/or sputa of the 167 persons investigated. In contrast, 41 sera were ascertained with antibodies against Paragonimus africanus using ELISA testing. As the optical density (OD values related to seropositive findings were found under 0.6 (the minimal OD to detect an active paragonimosis, the above antibody titres might originate from patients in chronic or in convalescent stages, or might result of cross reactions with trematodes. Concomitantly, dissection of local crabs (Callinectes marginatus demonstrated the presence of Paragonimus metacercariae in six out of 34 examined. The parasite burdens in crabs ranged from two to 35 cysts with a mean diameter of 302 μm. In Ivory Coast, the locality of Divo must be considered an at-risk zone in reason of the presence of anti-Paragonimus antibodies in several human sera and the presence of infected crabs at the local market.

  17. Advances in Developing Therapies to Combat Zika Virus: Current Knowledge and Future Perspectives

    Science.gov (United States)

    Munjal, Ashok; Khandia, Rekha; Dhama, Kuldeep; Sachan, Swati; Karthik, Kumaragurubaran; Tiwari, Ruchi; Malik, Yashpal S.; Kumar, Deepak; Singh, Raj K.; Iqbal, Hafiz M. N.; Joshi, Sunil K.

    2017-01-01

    Zika virus (ZIKV) remained largely quiescent for nearly six decades after its first appearance in 1947. ZIKV reappeared after 2007, resulting in a declaration of an international “public health emergency” in 2016 by the World Health Organization (WHO). Until this time, ZIKV was considered to induce only mild illness, but it has now been established as the cause of severe clinical manifestations, including fetal anomalies, neurological problems, and autoimmune disorders. Infection during pregnancy can cause congenital brain abnormalities, including microcephaly and neurological degeneration, and in other cases, Guillain-Barré syndrome, making infections with ZIKV a substantial public health concern. Genomic and molecular investigations are underway to investigate ZIKV pathology and its recent enhanced pathogenicity, as well as to design safe and potent vaccines, drugs, and therapeutics. This review describes progress in the design and development of various anti-ZIKV therapeutics, including drugs targeting virus entry into cells and the helicase protein, nucleosides, inhibitors of NS3 protein, small molecules, methyltransferase inhibitors, interferons, repurposed drugs, drugs designed with the aid of computers, neutralizing antibodies, convalescent serum, antibodies that limit antibody-dependent enhancement, and herbal medicines. Additionally, covalent inhibitors of viral protein expression and anti-Toll-like receptor molecules are discussed. To counter ZIKV-associated disease, we need to make rapid progress in developing novel therapies that work effectually to inhibit ZIKV. PMID:28824594

  18. Unsuspected Dengue as a Cause of Acute Febrile Illness in Children and Adults in Western Nicaragua.

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    Megan E Reller

    2016-10-01

    Full Text Available Dengue is an emerging infectious disease of global significance. Suspected dengue, especially in children in Nicaragua's heavily-urbanized capital of Managua, has been well documented, but unsuspected dengue among children and adults with undifferentitated fever has not.To prospectively study dengue in semi-urban and rural western Nicaragua, we obtained epidemiologic and clinical data as well as acute and convalescent sera (2 to 4 weeks after onset of illness from a convenience sample (enrollment Monday to Saturday daytime to early evening of consecutively enrolled patients (n = 740 aged ≥ 1 years presenting with acute febrile illness. We tested paired sera for dengue IgG and IgM and serotyped dengue virus using reverse transcriptase-PCR. Among 740 febrile patients enrolled, 90% had paired sera. We found 470 (63.5% were seropositive for dengue at enrollment. The dengue seroprevalance increased with age and reached >90% in people over the age of 20 years. We identified acute dengue (serotypes 1 and 2 in 38 (5.1% patients. Only 8.1% (3/37 of confirmed cases were suspected clinically.Dengue is an important and largely unrecognized cause of fever in rural western Nicaragua. Since Zika virus is transmitted by the same vector and has been associated with severe congenital infections, the population we studied is at particular risk for being devastated by the Zika epidemic that has now reached Central America.

  19. Clinical and hepatic evaluation in adult dengue patients: a prospective two-month cohort study

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    Ricardo Tristão-Sá

    2012-12-01

    Full Text Available INTRODUCTION: To analyze the liver dysfunction and evolution of signs and symptoms in adult dengue patients during a two-month follow-up period. METHODS: A prospective cohort study was conducted in Campos dos Goytacazes, Rio de Janeiro, Brazil, from January to July, 2008. The evolution of laboratory and clinical manifestations of 90 adult dengue patients was evaluated in five scheduled visits within a two-month follow-up period. Twenty controls were enrolled for the analysis of liver function. Patients with hepatitis B, hepatitis C, those known to be human immunodeficiency virus (HIV seropositive and pregnant women were excluded from the study. RESULTS: At the end of the second month following diagnosis, we observed that symptoms persisted in 33.3% (30/90 of dengue patients. We also observed that, 57.7% (15/26 of the symptoms persisted at the end of the second month. The most persistent symptoms were arthralgia, fatigue, weakness, adynamia, anorexia, taste alteration, and hair loss. Prior dengue virus (DENV infection did not predispose patients to a longer duration of symptoms. Among hepatic functions, transaminases had the most remarkable elevation and in some cases remained elevated up to the second month after the disease onset. Alanine aminotransferase (ALT levels overcame aspartate aminotransferase (AST during the convalescent period. Male patients were more severely affected than females. CONCLUSIONS: Dengue fever may present a wide number of symptoms and elevated liver transaminases at the end of the second month.

  20. Utility of C-Reactive Protein Levels for Early Prediction of Dengue Severity in Adults

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    Chien-Chih Chen

    2015-01-01

    Full Text Available Dengue has broad clinical presentation with unpredictable clinical evolution and outcome. We aimed to evaluate the utility of C-reactive protein (CRP levels for distinguishing between mild and severe cases in the early phase of the dengue illness. We retrospectively evaluated adults with dengue from 2006 to 2014, according to 1997 and 2009 World Health Organization (WHO criteria for severity. Of 191 included patients, 32.9% had nonshock dengue hemorrhagic fever (DHF, 3.1% dengue shock syndrome (DSS, and 7.9% severe dengue. The risk of DHF/DSS and severe dengue is significantly related to the increasing levels of CRP. Of 191 patients, 97 had CRP levels measured during the febrile (days 1–3; 85 during the critical (days 4–6; and 9 during the convalescent (days 7–10 illness phases. During the febrile phase, there was significant higher CRP level for DSS versus DF/nonshock DHF and severe dengue versus nonsevere dengue, with CRP cutoff level 30.1 mg/L (area under the receiver operating characteristic curve (AUC, 0.938; 100% sensitivity, 76.3% specificity and 24.2 mg/L (AUC, 0.717; 70% sensitivity, 71.3% specificity, respectively. Our study highlights the utility of the CRP levels in early prediction of DSS and severe dengue in adult patients.

  1. Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display

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    Meyer Torsten

    2012-06-01

    Full Text Available Abstract Background Solely in Europoe, Salmonella Typhimurium causes more than 100,000 infections per year. Improved detection of livestock colonised with S. Typhimurium is necessary to prevent foodborne diseases. Currently, commercially available ELISA assays are based on a mixture of O-antigens (LPS or total cell lysate of Salmonella and are hampered by cross-reaction. The identification of novel immunogenic proteins would be useful to develop ELISA based diagnostic assays with a higher specificity. Results A phage display library of the entire Salmonella Typhimurium genome was constructed and 47 immunogenic oligopeptides were identified using a pool of convalescent sera from pigs infected with Salmonella Typhimurium. The corresponding complete genes of seven of the identified oligopeptids were cloned. Five of them were produced in E. coli. The immunogenic character of these antigens was validated with sera from pigs infeced with S. Tyhimurium and control sera from non-infected animals. Finally, human antibody fragments (scFv against these five antigens were selected using antibody phage display and characterised. Conclusion In this work, we identified novel immunogenic proteins of Salmonella Typhimurium and generated antibody fragments against these antigens completely based on phage display. Five immunogenic proteins were validated using a panel of positive and negative sera for prospective applications in diagnostics of Salmonela Typhimurium.

  2. The experiences and needs of Gujarati Hindu patients and partners in the first month after a myocardial infarction.

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    Webster, Rosemary A; Thompson, David R; Mayou, Richard A

    2002-02-01

    South Asian people living in the United Kingdom are at increased risk of coronary heart disease, have higher mortality rates and are less likely to be treated when compared to the white population. There is, however, little information about the experiences and needs of this group after discharge from hospital. To explore the experiences and needs of Gujarati Hindu patients and their partners in the first month after a myocardial infarction. Using a qualitative research approach, semi-structured interviews were conducted by a Gujarati-speaking researcher with 35 patients and their partners at home during early convalescence. A number of categories emerged from the data which pertained to a lack of information and advice, poor performance of activity, little lifestyle adjustment, poor expectations, lack of future plans, strong family support, dissatisfaction with the family doctor, and a significant belief in fate. Experiences and health care needs of Gujarati Hindu patients with myocardial infarction appear different to those of non-Asians. Cardiac aftercare and rehabilitation services should take account of such information

  3. Symptom-limited maximal treadmill testing after myocardial infarction.

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    Roberts, K C; Logan, R L

    1980-11-12

    In this paper we report our experience of routine symptom limited maximal treadmill assessment, and the methodology used with patients at the end of their convalescence after myocardial infarction. Sixty-one of 68 (90 percent) consecutive patients, mean age 55.7 years (21 to 69 years), were studied at the median time after infarction of six weeks (three to 16 weeks). No complications occurred during or after the tests. Fifty-six percent of the patients studied achieved a work capacity which was within the average range reported for healthy people of the smae age. Thirty-two percent experienced chest pain thought to be angina and 31 percent developed ST segment depression of at least 1 mm without chest pain. Although ventricular premature beats occurred in half the tests the only arrhythmia requiring any treatment was a supraventricular tachycardia. The assessment of work capacity and limiting symptoms in this way after myocardial infarction is safe and is of considerable help in patient management.

  4. Immunoproteomic analysis of bacterial proteins of Actinobacillus pleuropneumoniae serotype 1

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    Wu Zongfu

    2011-06-01

    Full Text Available Abstract Background Actinobacillus pleuropneumoniae (APP is one of the most important swine pathogens worldwide. Identification and characterization of novel antigenic APP vaccine candidates are underway. In the present study, we use an immunoproteomic approach to identify APP protein antigens that may elicit an immune response in serotype 1 naturally infected swine and serotype 1 virulent strain S259-immunized rabbits. Results Proteins from total cell lysates of serotype 1 APP were separated by two-dimensional electrophoresis (2DE. Western blot analysis revealed 21 immunoreactive protein spots separated in the pH 4-7 range and 4 spots in the pH 7-11 range with the convalescent sera from swine; we found 5 immunoreactive protein spots that separated in the pH 4-7 range and 2 in the pH 7-11 range with hyperimmune sera from S259-immunized rabbits. The proteins included the known antigens ApxIIA, protective surface antigen D15, outer membrane proteins P5, subunit NqrA. The remaining antigens are being reported as immunoreactive proteins in APP for the first time, to our knowledge. Conclusions We identified a total of 42 immunoreactive proteins of the APP serotype 1 virulent strain S259 which represented 32 different proteins, including some novel immunoreactive factors which could be researched as vaccine candidates.

  5. Multidrug-resistant typhoid fever with neurologic findings on the Malawi-Mozambique border.

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    Lutterloh, Emily; Likaka, Andrew; Sejvar, James; Manda, Robert; Naiene, Jeremias; Monroe, Stephan S; Khaila, Tadala; Chilima, Benson; Mallewa, Macpherson; Kampondeni, Sam D; Lowther, Sara A; Capewell, Linda; Date, Kashmira; Townes, David; Redwood, Yanique; Schier, Joshua G; Nygren, Benjamin; Tippett Barr, Beth; Demby, Austin; Phiri, Abel; Lungu, Rudia; Kaphiyo, James; Humphrys, Michael; Talkington, Deborah; Joyce, Kevin; Stockman, Lauren J; Armstrong, Gregory L; Mintz, Eric

    2012-04-01

    Salmonella enterica serovar Typhi causes an estimated 22 million cases of typhoid fever and 216 000 deaths annually worldwide. We investigated an outbreak of unexplained febrile illnesses with neurologic findings, determined to be typhoid fever, along the Malawi-Mozambique border. The investigation included active surveillance, interviews, examinations of ill and convalescent persons, medical chart reviews, and laboratory testing. Classification as a suspected case required fever and ≥1 other finding (eg, headache or abdominal pain); a probable case required fever and a positive rapid immunoglobulin M antibody test for typhoid (TUBEX TF); a confirmed case required isolation of Salmonella Typhi from blood or stool. Isolates underwent antimicrobial susceptibility testing and subtyping by pulsed-field gel electrophoresis (PFGE). We identified 303 cases from 18 villages with onset during March-November 2009; 214 were suspected, 43 were probable, and 46 were confirmed cases. Forty patients presented with focal neurologic abnormalities, including a constellation of upper motor neuron signs (n = 19), ataxia (n = 22), and parkinsonism (n = 8). Eleven patients died. All 42 isolates tested were resistant to ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazole; 4 were also resistant to nalidixic acid. Thirty-five of 42 isolates were indistinguishable by PFGE. The unusual neurologic manifestations posed a diagnostic challenge that was resolved through rapid typhoid antibody testing in the field and subsequent blood culture confirmation in the Malawi national reference laboratory. Extending laboratory diagnostic capacity, including blood culture, to populations at risk for typhoid fever in Africa will improve outbreak detection, response, and clinical treatment.

  6. Endoscopic and laparoscopic treatment of gastroesophageal reflux.

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    Watson, David I; Immanuel, Arul

    2010-04-01

    Gastroesophageal reflux is extremely common in Western countries. For selected patients, there is an established role for the surgical treatment of reflux, and possibly an emerging role for endoscopic antireflux procedures. Randomized trials have compared medical versus surgical management, laparoscopic versus open surgery and partial versus total fundoplications. However, the evidence base for endoscopic procedures is limited to some small sham-controlled studies, and cohort studies with short-term follow-up. Laparoscopic fundoplication has been shown to be an effective antireflux operation. It facilitates quicker convalescence and is associated with fewer complications, but has a similar longer term outcome compared with open antireflux surgery. In most randomized trials, antireflux surgery achieves at least as good control of reflux as medical therapy, and these studies support a wider application of surgery for the treatment of moderate-to-severe reflux. Laparoscopic partial fundoplication is an effective surgical procedure with fewer side effects, and it may achieve high rates of patient satisfaction at late follow-up. Many of the early endoscopic antireflux procedures have failed to achieve effective reflux control, and they have been withdrawn from the market. Newer procedures have the potential to fashion a surgical fundoplication. However, at present there is insufficient evidence to establish the safety and efficacy of endoscopic procedures for the treatment of gastroesophageal reflux, and no endoscopic procedure has achieved equivalent reflux control to that achieved by surgical fundoplication.

  7. Septic osteitis of the distal phalanx: findings and surgical treatment in 18 cases.

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    Cauvin, E R; Munroe, G A

    1998-11-01

    Eighteen cases of septic osteitis of the distal phalanx were diagnosed clinically and confirmed at surgery over a 6 year period (1990-1996). Focal bone lysis was the most common radiographic finding. Sequestra were identified in 4 horses, one of which was associated with a fractured plantar process. All the horses were treated surgically with open debridement. The septic process recurred in 4 horses: one was subjected to euthanasia due to navicular bursa infection, secondary to a pathological fracture, the other 3 horses responded to further debridement. A pathological fracture occurred in another horse. All the survivors were not lame 3 months post operatively, although complete healing took 1-12 months. Long term follow up was available in 14 cases. One was retired, all the other horses were back in work at a similar level of performance. This series indicates that surgical treatment of septic pedal osteitis carries a fair to good prognosis despite a high rate of post operative complications and convalescence period of up to 12 months.

  8. Successful preoperative diagnosis of the localization of adenoma of the parathyroid gland by scintiscan

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    Takei, Ko; Shirasu, Nobuhiko; Kobayashi, Katsumi; Komatsu, Hideki; Ueno, Akira; Uchiyama, Akira

    1985-02-01

    Successful preoperative diagnosis of the localization of adenoma of the parathyroid in 59-year-old female with hyperparathyroidism by means of double tracer scanning and computerized image subtraction was described. She had been suffering from recurrent urolithiasis and was referred to our clinic because of hypercalcemia and hypophosphatemia in November 1983. The level of serum calcium was 11.2 mg/dl and inorganic serum phosphorus 2.1 mg/dl. The activity of serum alkaline phosphatase was within normal limits. Urinary calcium excretion was elevated to the level of 262 mg/day under low calcium diet (300 mg/day). CT scan could not detect adenoma of the parathyroid. Double tracer scanning with S TI as chloride salt and /sup 99m/Tc as pertechnatate and computerized image subtraction showed abnormal thallium uptake about the lower portion of the right lobe of the thyroid gland. Neck exploration revealed a parathyroid adenoma, weighing 1.0 g behind the right lobe of the thyroid gland as was demonstrated by the preoperative subtracted scintiscan. Convalescence was uneventful and the level of serum calcium and urinary calcium excretion was normalized.

  9. Cytokine Profiles during Invasive Nontyphoidal Salmonella Disease Predict Outcome in African Children

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    Gilchrist, James J.; Heath, Jennifer N.; Msefula, Chisomo L.; Gondwe, Esther N.; Naranbhai, Vivek; Mandala, Wilson; MacLennan, Jenny M.; Molyneux, Elizabeth M.; Graham, Stephen M.; Drayson, Mark T.; Molyneux, Malcolm E.

    2016-01-01

    Nontyphoidal Salmonella is a leading cause of sepsis in African children. Cytokine responses are central to the pathophysiology of sepsis and predict sepsis outcome in other settings. In this study, we investigated cytokine responses to invasive nontyphoidal Salmonella (iNTS) disease in Malawian children. We determined serum concentrations of 48 cytokines with multiplexed immunoassays in Malawian children during acute iNTS disease (n = 111) and in convalescence (n = 77). Principal component analysis and logistic regression were used to identify cytokine signatures of acute iNTS disease. We further investigated whether these responses are altered by HIV coinfection or severe malnutrition and whether cytokine responses predict inpatient mortality. Cytokine changes in acute iNTS disease were associated with two distinct cytokine signatures. The first is characterized by increased concentrations of mediators known to be associated with macrophage function, and the second is characterized by raised pro- and anti-inflammatory cytokines typical of responses reported in sepsis secondary to diverse pathogens. These cytokine responses were largely unaltered by either severe malnutrition or HIV coinfection. Children with fatal disease had a distinctive cytokine profile, characterized by raised mediators known to be associated with neutrophil function. In conclusion, cytokine responses to acute iNTS infection in Malawian children are reflective of both the cytokine storm typical of sepsis secondary to diverse pathogens and the intramacrophage replicative niche of NTS. The cytokine profile predictive of fatal disease supports a key role of neutrophils in the pathogenesis of NTS sepsis. PMID:27170644

  10. Lymphocyte Perturbations in Malawian Children with Severe and Uncomplicated Malaria.

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    Mandala, Wilson L; Msefula, Chisomo L; Gondwe, Esther N; Gilchrist, James J; Graham, Stephen M; Pensulo, Paul; Mwimaniwa, Grace; Banda, Meraby; Taylor, Terrie E; Molyneux, Elizabeth E; Drayson, Mark T; Ward, Steven A; Molyneux, Malcolm E; MacLennan, Calman A

    2015-11-18

    Lymphocytes are implicated in immunity and pathogenesis of severe malaria. Since lymphocyte subsets vary with age, assessment of their contribution to different etiologies can be difficult. We immunophenotyped peripheral blood from Malawian children presenting with cerebral malaria, severe malarial anemia, and uncomplicated malaria (n = 113) and healthy aparasitemic children (n = 42) in Blantyre, Malawi, and investigated lymphocyte subset counts, activation, and memory status. Children with cerebral malaria were older than those with severe malarial anemia. We found panlymphopenia in children presenting with cerebral malaria (median lymphocyte count, 2,100/μl) and uncomplicated malaria (3,700/μl), which was corrected in convalescence and was absent in severe malarial anemia (5,950/μl). Median percentages of activated CD69(+) NK (73%) and γδ T (60%) cells were higher in cerebral malaria than in other malaria types. Median ratios of memory to naive CD4(+) lymphocytes were higher in cerebral malaria than in uncomplicated malaria and low in severe malarial anemia. The polarized lymphocyte subset profiles of different forms of severe malaria are independent of age. In conclusion, among Malawian children cerebral malaria is characterized by lymphocyte activation and increased memory cells, consistent with immune priming. In contrast, there are reduced memory cells and less activation in severe malaria anemia. Further studies are required to understand whether these immunological profiles indicate predisposition of some children to one or another form of severe malaria. Copyright © 2016 Mandala et al.

  11. A visit paid to Jung by Alwine von Keller.

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    Bernardini, Riccardo; Quaglino, Gian Piero; Romano, Augusto

    2011-04-01

    In the winter of 1943-1944, Jung had suffered a coronary thrombosis which almost cost him his life. During his illness, Jung experienced a series of visions, described in his Memories, Dreams, Reflections, which were also to influence significantly the development of his theoretical thinking. On 27(th) September 1944, Alwine von Keller (1878-1965) paid a visit to Jung, while he was still convalescing, in Zurich and documented her meeting with him in a series of notes, recently discovered, which testify to the fact that, at the time of their meeting, Jung was engaged in writing the 'Salt' chapter of Mysterium coniunctionis and investigating the alchemistic symbolism of the 'sea'. This theme seems to testify to a continuity of interests on Jung's part with the seminar he held at Eranos the previous year on the cartographic art of Opicinus de Canistris (1296-c.1352). With its addition of many unpublished details, Alwine von Keller's notes supplement the report which Jung made of his visions experienced during his sickness in MDR. In particular, these attest to the fact that Jung had attributed the terrible experience which he had endured to the problem of the conjunctio, which was confronting him from the theoretical point of view in his writing of Mysterium coniunctionis.

  12. Effect of physical therapy frequency on gross motor function in children with cerebral palsy.

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    Park, Eun-Young

    2016-06-01

    [Purpose] This study attempted to investigate the effect of physical therapy frequency based on neurodevelopmental therapy on gross motor function in children with cerebral palsy. [Subjects and Methods] The study sample included 161 children with cerebral palsy who attended a convalescent or rehabilitation center for disabled individuals or a special school for children with physical disabilities in South Korea. Gross Motor Function Measure data were collected according to physical therapy frequency based on neurodevelopmental therapy for a period of 1 year. [Results] The correlation between physical therapy frequency and Gross Motor Function Measure scores for crawling and kneeling, standing, walking, running and jumping, and rolling, and the Gross Motor Function Measure total score was significant. The differences in gross motor function according to physical therapy frequency were significant for crawling, kneeling, standing, and Gross Motor Function Measure total score. The differences in gross motor function according to frequency of physical therapy were significant for standing in Gross Motor Function Classification System Level V. [Conclusion] Intensive physical therapy was more effective for improving gross motor function in children with cerebral palsy. In particular, crawling and kneeling, and standing ability showed greater increases with intensive physical therapy.

  13. Heterogeneity of Campylobacter species isolated from serial stool specimens of Egyptian children using pulsed field gel electrophoresis

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    Atef M. El-Gendy

    2013-03-01

    Full Text Available Background: The genus Campylobacter spp. is a common cause of human acute bacteria lenteritis and travellers’ diarrhoea worldwide.Objective: To determine whether multiple serial isolations of Campylobacter spp., when obtained from a single child, represented the same or a different organism.Methods: In a birth cohort study conducted in Egypt, numerous children showed serial isolations of Campylobacter spp. Of these, 13 children were selected from different households based on the successive isolation of six or more Campylobacter isolates from stool samples.Results: Eighty isolates were recovered and identified as either Campylobacter coli (n = 25 or Campylobacter jejuni (n = 55. Pulsed-field gel electrophoresis (PFGE revealed the presence of 38 unique C. jejuni and 24 C. coli profiles at a similarity level of ≥ 90%. Although no seriallyidentical isolates were detected in six children, others demonstrated at least one identical couple of isolates; all identified serially between one to six weeks. Two children demonstrated > 80% similar couples of isolates that appeared seven months apart. PFGE could be a useful tool for differentiating reinfection, relapse and convalescent excretion phases.Conclusion: Our data suggest that Campylobacter infection in children is a complex process; children are exposed to multiple species in endemic environments and strains of the same bacterium appear to be shed serially between one to six weeks after the first exposure. Isolates that persisted for longer periods were relatively less similar, as shown from the results of this study.

  14. Empyema and Respiratory Failure Secondary to Nephropleural Fistula Caused by Chronic Urinary Tract Infection: A Case Report

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    G. H. Jones

    2012-01-01

    Full Text Available We report a case of nephropleural fistula causing empyema and respiratory failure in a 68-year-old gentleman with a long history of urological problems including recurrent nephrolithiasis and urinary tract infections. He was admitted with sepsis, a productive cough, pyuria, and reduced breath sounds over the left hemithorax. Radiological imaging revealed a fistulous connection between a left-sided perinephric abscess and the pleural space. He was commenced on broad spectrum intravenous antibiotics but developed progressive respiratory failure requiring intensive care admission. Urinary and pleural aspirates cultured facultative anaerobic pathogens with identical resistance patterns. Drainage of thoracic and perinephric collections was carried out, allowing him to be extubated after 24 hours and discharged home after 18 days on an extended course of oral antibiotics. Left nephrectomy is now planned after a period of convalescence. Empyema developing in patients with known urolithiasis should alert the treating physician to the possibility that a pathological communication has formed especially if typical urinary tract pathogens are cultured from respiratory sampling.

  15. Transvesicoscopic Repair of Vesicovaginal Fistula

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    R. B. Nerli

    2010-01-01

    Full Text Available Introduction. Vesicovaginal fistula has been a social and surgical problem for centuries. Many surgical techniques have been developed to correct this abnormality, including transabdominal, transvaginal, and endoscopic approaches. The best approach is probably the one with which the surgeon feels most experienced and comfortable. Laparoscopy has become increasingly popular in urology, reducing the invasiveness of treatment and shortening the period of convalescence. We report our results of transvesicoscopic approach for VVF repair. Materials and Methods. Patients with VVF were offered repair using the transvesicoscopic route. With the patient under general anaesthesia and in modified lithotomy position cystoscopy was performed with gas insufflation. Under cystoscopic guidance the bladder was fixed to anterior abdominal wall and ports inserted into the bladder. The fistula was repaired under endoscopic vision. Results. Four women, who had VVF following abdominal hysterectomy, underwent this procedure. The operating time ranged from 175 to 235 minutes. There was minimal bleeding. Post operative complications included ileus in one and fever in another. No recurrence of VVF was noted in any patient. Conclusions. Transvesicoscopic repair of VVF is feasible, safe, and results in lower morbidity and quicker recovery time.

  16. Features of Two New Proteins with OmpA-Like Domains Identified in the Genome Sequences of Leptospira interrogans

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    Teixeira, Aline F.; de Morais, Zenaide M.; Kirchgatter, Karin; Romero, Eliete C.; Vasconcellos, Silvio A.; Nascimento, Ana Lucia T. O.

    2015-01-01

    Leptospirosis is an acute febrile disease caused by pathogenic spirochetes of the genus Leptospira. It is considered an important re-emerging infectious disease that affects humans worldwide. The knowledge about the mechanisms by which pathogenic leptospires invade and colonize the host remains limited since very few virulence factors contributing to the pathogenesis of the disease have been identified. Here, we report the identification and characterization of two new leptospiral proteins with OmpA-like domains. The recombinant proteins, which exhibit extracellular matrix-binding properties, are called Lsa46 - LIC13479 and Lsa77 - LIC10050 (Leptospiral surface adhesins of 46 and 77 kDa, respectively). Attachment of Lsa46 and Lsa77 to laminin was specific, dose dependent and saturable, with KD values of 24.3 ± 17.0 and 53.0 ± 17.5 nM, respectively. Lsa46 and Lsa77 also bind plasma fibronectin, and both adhesins are plasminogen (PLG)-interacting proteins, capable of generating plasmin (PLA) and as such, increase the proteolytic ability of leptospires. The proteins corresponding to Lsa46 and Lsa77 are present in virulent L. interrogans L1-130 and in saprophyte L. biflexa Patoc 1 strains, as detected by immunofluorescence. The adhesins are recognized by human leptospirosis serum samples at the onset and convalescent phases of the disease, suggesting that they are expressed during infection. Taken together, our data could offer valuable information to the understanding of leptospiral pathogenesis. PMID:25849456

  17. Laparoscopic donor nephrectomy

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    Gupta Nitin

    2005-01-01

    Full Text Available Of the various options for patients with end stage renal disease, kidney transplantation is the treatment of choice for a suitable patient. The kidney for transplantation is retrieved from either a cadaver or a live donor. Living donor nephrectomy has been developed as a method to address the shortfall in cadaveric kidneys available for transplantation. Laparoscopic living donor nephrectomy (LLDN, by reducing postoperative pain, shortening convalescence, and improving the cosmetic outcome of the donor nephrectomy, has shown the potential to increase the number of living kidney donations further by removing some of the disincentives inherent to donation itself. The technique of LLDN has undergone evolution at different transplant centers and many modifications have been done to improve donor safety and recipient outcome. Virtually all donors eligible for an open surgical procedure may also undergo the laparoscopic operation. Various earlier contraindications to LDN, such as right donor kidney, multiple vessels, anomalous vasculature and obesity have been overcome with increasing experience. Laparoscopic live donor nephrectomy can be done transperitoneally or retroperitoneally on either side. The approach is most commonly transperitoneal, which allows adequate working space and easy dissection. A review of literature and our experience with regards to standard approach and the modifications is presented including a cost saving model for the developing countries. An assessment has been made, of the impact of LDN on the outcome of donor and the recipient.

  18. Experimental infection of laying hens with Salmonella enterica serovar Gallinarum Infecção experimental com Salmonella enterica serovar Gallinarum em poedeiras comerciais

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    Gláucia Helaine de Oliveira

    2005-03-01

    Full Text Available Experimental infections were set up in commercial laying birds, comprising a white relatively resistant line and a red susceptible line infecting with Salmonella enterica serovar Gallinarum. The major findings were that in susceptible birds clinical disease occurred in a dose-dependent manner. Faecal excretion occurred in susceptible birds almost up to death but also occurred in the more resistant line and in birds, which were convalescing. Removal of birds, which had died from the disease, from the environment, reduced the resultant mortality/morbidity and may be regarded as a useful measure for control.Infecções experimentais por Salmonella enterica serovar Gallinarum foram realizadas em aves de postura comercial, incluindo uma linhagem branca resistente e uma linhagem vermelha susceptível ao desenvolvimento da enfermidade clínica. As aves de linhagem susceptível apresentaram doença clínica dependente da dose administrada. Excreção fecal foi observada em aves da linhagem susceptível próximo ao momento da morte e, eventualmente, em aves da linhagem resistente e aves convalescentes. A remoção das aves mortas do meio ambiente reduziu a taxa de mortalidade/morbidade, procedimento este que pode ser utilizado como medida de controle.

  19. Acute phase reactants, challenge in the near future of animal production and veterinary medicine

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The future of acute phase proteins (APPs) in science is discussed in this paper. Many functions and associated pathological processes of APPs are unknown. Extrahepatic formation in local tissues needs attention. Local serum amyloid A (SAA) formation may be involved in deposition of AA-amyloid induced by conformational change of SAA resulting in amyloid formation, having tremendous food safety implications. Amyloidogenesis is enhanced in mouse fed beta pleated sheet-rich proteins. The local amyloid in joints of chicken and mammary corpora amylacea is discussed. Differences in glycosylation of glycoproteins among the APPs, as has been shown for α1-acid glycoprotein, have to be considered. More knowledge on the reactivity patterns may lead to implication of APPs in the diagnostics and staging of a disease. Calculation of an index from values of several acute phase variables increases the power of APPs in monitoring unhealthy individuals in animal populations.Vaccinations, just as infections in eliciting acute phase response seem to limit the profitability of vaccines because acute phase reactions are contraproductive in view of muscle anabolism. Interest is focused on amino acid patterns and vitamins in view of dietary nutrition effect on sick and convalescing animals.When inexpensive methodology such as liquid phase methods (nephelometry, turbidimetry) or protein array technology for rapid APPs measurement is available, APPs have a future in routine diagnostics. Specific groups of patients may be screened or populations monitored by using APPs.

  20. [Successive ruptures of patellar and Achilles tendons. Anabolic steroids in competitive sports].

    Science.gov (United States)

    Isenberg, J; Prokop, A; Skouras, E

    2008-01-01

    Derivatives of testosterone or of 19-nor-testosterone are used as anabolics for the purpose of improving performance although the effect of anabolics is known still to be under discussion. The use of anabolic steroids continues among competitive athletes despite increased controls and increasingly frequent dramatic incidents connected with them. Whereas metabolic dysfunction during anabolic use is well documented, ruptures of the large tendons are rarely reported. Within 18 months, a 29-year-old professional footballer needed surgery for rupture of the patellar tendon and of both Achilles tendons. Carefully directed questioning elicited confirmation that he had taken different anabolic steroids regularly for 3 years with the intention of improving his strength. After each operation anabolic steroids were taken again at a high dosage during early convalescence and training. Minimally invasive surgery and open suturing techniques led to complete union of the Achilles tendons in good time. Training and anabolic use (metenolon 300 mg per week) started early after suturing of the patellar tendon including bone tunnels culminated in histologically confirmed rerupture after 8 weeks. After a ligament reconstruction with a semitendinosus tendon graft with subsequent infection, the tendon and reserve traction apparatus were lost. Repeated warnings of impaired healing if anabolic use was continued had been given without success. In view of the high number of unrecorded cases in competitive and athletic sports, we can assume that the use of anabolic steroids is also of quantitative relevance in the operative treatment of tendon ruptures.