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Sample records for complement system components

  1. Autoantibodies against complement components in systemic lupus erythematosus - role in the pathogenesis and clinical manifestations.

    Science.gov (United States)

    Hristova, M H; Stoyanova, V S

    2017-12-01

    Many complement structures and a number of additional factors, i.e. autoantibodies, receptors, hormones and cytokines, are implicated in the complex pathogenesis of systemic lupus erythematosus. Genetic defects in the complement as well as functional deficiency due to antibodies against its components lead to different pathological conditions, usually clinically presented. Among them hypocomplementemic urticarial vasculitis, different types of glomerulonephritis as dense deposit disease, IgA nephropathy, atypical haemolytic uremic syndrome and lupus nephritis are very common. These antibodies cause conformational changes leading to pathological activation or inhibition of complement with organ damage and/or limited capacity of the immune system to clear immune complexes and apoptotic debris. Finally, we summarize the role of complement antibodies in the pathogenesis of systemic lupus erythematosus and discuss the mechanism of some related clinical conditions such as infections, thyroiditis, thrombosis, acquired von Willebrand disease, etc.

  2. conformational complexity of complement component C3

    NARCIS (Netherlands)

    Janssen, B.J.C.

    2007-01-01

    The complement system is an important part of the immune system and critical for the elimination of pathogens. In mammals the complement system consists of an intricate set of about 35 soluble and cell-surface plasma proteins. Central to complement is component C3, a large protein of 1,641 residues.

  3. Complement component 4

    Science.gov (United States)

    ... the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic ... with an autoimmune disorder . For example, people with active systemic lupus erythematosus may have lower-than-normal ...

  4. Early Components of the Complement Classical Activation Pathway in Human Systemic Autoimmune Diseases

    Science.gov (United States)

    Lintner, Katherine E.; Wu, Yee Ling; Yang, Yan; Spencer, Charles H.; Hauptmann, Georges; Hebert, Lee A.; Atkinson, John P.; Yu, C. Yung

    2016-01-01

    The complement system consists of effector proteins, regulators, and receptors that participate in host defense against pathogens. Activation of the complement system, via the classical pathway (CP), has long been recognized in immune complex-mediated tissue injury, most notably systemic lupus erythematosus (SLE). Paradoxically, a complete deficiency of an early component of the CP, as evidenced by homozygous genetic deficiencies reported in human, are strongly associated with the risk of developing SLE or a lupus-like disease. Similarly, isotype deficiency attributable to a gene copy-number (GCN) variation and/or the presence of autoantibodies directed against a CP component or a regulatory protein that result in an acquired deficiency are relatively common in SLE patients. Applying accurate assay methodologies with rigorous data validations, low GCNs of total C4, and heterozygous and homozygous deficiencies of C4A have been shown as medium to large effect size risk factors, while high copy numbers of total C4 or C4A as prevalent protective factors, of European and East-Asian SLE. Here, we summarize the current knowledge related to genetic deficiency and insufficiency, and acquired protein deficiencies for C1q, C1r, C1s, C4A/C4B, and C2 in disease pathogenesis and prognosis of SLE, and, briefly, for other systemic autoimmune diseases. As the complement system is increasingly found to be associated with autoimmune diseases and immune-mediated diseases, it has become an attractive therapeutic target. We highlight the recent developments and offer a balanced perspective concerning future investigations and therapeutic applications with a focus on early components of the CP in human systemic autoimmune diseases. PMID:26913032

  5. Genetics Home Reference: complement component 2 deficiency

    Science.gov (United States)

    ... Topic: Immune System and Disorders Health Topic: Lupus Genetic and Rare Diseases Information Center (1 link) Complement component 2 deficiency Additional NIH Resources (1 link) National Institute of Allergy and Infectious Diseases: Primary Immune Deficiency Diseases Educational Resources (6 ...

  6. Characterization of a Gene Coding for the Complement System Component FB from Loxosceles laeta Spider Venom Glands.

    Science.gov (United States)

    Myamoto, Daniela Tiemi; Pidde-Queiroz, Giselle; Gonçalves-de-Andrade, Rute Maria; Pedroso, Aurélio; van den Berg, Carmen W; Tambourgi, Denise V

    2016-01-01

    The human complement system is composed of more than 30 proteins and many of these have conserved domains that allow tracing the phylogenetic evolution. The complement system seems to be initiated with the appearance of C3 and factor B (FB), the only components found in some protostomes and cnidarians, suggesting that the alternative pathway is the most ancient. Here, we present the characterization of an arachnid homologue of the human complement component FB from the spider Loxosceles laeta. This homologue, named Lox-FB, was identified from a total RNA L. laeta spider venom gland library and was amplified using RACE-PCR techniques and specific primers. Analysis of the deduced amino acid sequence and the domain structure showed significant similarity to the vertebrate and invertebrate FB/C2 family proteins. Lox-FB has a classical domain organization composed of a control complement protein domain (CCP), a von Willebrand Factor domain (vWFA), and a serine protease domain (SP). The amino acids involved in Mg2+ metal ion dependent adhesion site (MIDAS) found in the vWFA domain in the vertebrate C2/FB proteins are well conserved; however, the classic catalytic triad present in the serine protease domain is not conserved in Lox-FB. Similarity and phylogenetic analyses indicated that Lox-FB shares a major identity (43%) and has a close evolutionary relationship with the third isoform of FB-like protein (FB-3) from the jumping spider Hasarius adansoni belonging to the Family Salcitidae.

  7. Characterization of a Gene Coding for the Complement System Component FB from Loxosceles laeta Spider Venom Glands.

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    Daniela Tiemi Myamoto

    Full Text Available The human complement system is composed of more than 30 proteins and many of these have conserved domains that allow tracing the phylogenetic evolution. The complement system seems to be initiated with the appearance of C3 and factor B (FB, the only components found in some protostomes and cnidarians, suggesting that the alternative pathway is the most ancient. Here, we present the characterization of an arachnid homologue of the human complement component FB from the spider Loxosceles laeta. This homologue, named Lox-FB, was identified from a total RNA L. laeta spider venom gland library and was amplified using RACE-PCR techniques and specific primers. Analysis of the deduced amino acid sequence and the domain structure showed significant similarity to the vertebrate and invertebrate FB/C2 family proteins. Lox-FB has a classical domain organization composed of a control complement protein domain (CCP, a von Willebrand Factor domain (vWFA, and a serine protease domain (SP. The amino acids involved in Mg2+ metal ion dependent adhesion site (MIDAS found in the vWFA domain in the vertebrate C2/FB proteins are well conserved; however, the classic catalytic triad present in the serine protease domain is not conserved in Lox-FB. Similarity and phylogenetic analyses indicated that Lox-FB shares a major identity (43% and has a close evolutionary relationship with the third isoform of FB-like protein (FB-3 from the jumping spider Hasarius adansoni belonging to the Family Salcitidae.

  8. The two-component system VicRK regulates functions associated with Streptococcus mutans resistance to complement immunity.

    Science.gov (United States)

    Alves, Livia A; Harth-Chu, Erika N; Palma, Thais H; Stipp, Rafael N; Mariano, Flávia S; Höfling, José F; Abranches, Jacqueline; Mattos-Graner, Renata O

    2017-10-01

    Streptococcus mutans, a dental caries pathogen, can promote systemic infections upon reaching the bloodstream. The two-component system (TCS) VicRK Sm of S. mutans regulates the synthesis of and interaction with sucrose-derived exopolysaccharides (EPS), processes associated with oral and systemic virulence. In this study, we investigated the mechanisms by which VicRK Sm affects S. mutans susceptibility to blood-mediated immunity. Compared with parent strain UA159, the vicK Sm isogenic mutant (UAvic) showed reduced susceptibility to deposition of C3b of complement, low binding to serum immunoglobulin G (IgG), and low frequency of C3b/IgG-mediated opsonophagocytosis by polymorphonuclear cells in a sucrose-independent way (Pmutans employs mechanisms of complement evasion through peptidases, which are controlled by VicRK Sm. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  9. The Epidermal Growth Factor Receptor Is a Regulator of Epidermal Complement Component Expression and Complement Activation

    DEFF Research Database (Denmark)

    Abu-Humaidan, Anas H A; Ananthoju, Nageshwar; Mohanty, Tirthankar

    2014-01-01

    The complement system is activated in response to tissue injury. During wound healing, complement activation seems beneficial in acute wounds but may be detrimental in chronic wounds. We found that the epidermal expression of many complement components was only increased to a minor extent in skin...

  10. Interactions of PLGA nanoparticles with blood components: protein adsorption, coagulation, activation of the complement system and hemolysis studies.

    Science.gov (United States)

    Fornaguera, Cristina; Calderó, Gabriela; Mitjans, Montserrat; Vinardell, Maria Pilar; Solans, Conxita; Vauthier, Christine

    2015-04-14

    The intravenous administration of poly(lactic-co-glycolic) acid (PLGA) nanoparticles has been widely reported as a promising alternative for delivery of drugs to specific cells. However, studies on their interaction with diverse blood components using different techniques are still lacking. Therefore, in the present work, the interaction of PLGA nanoparticles with blood components was described using different complementary techniques. The influence of different encapsulated compounds/functionalizing agents on these interactions was also reported. It is worth noting that all these techniques can be simply performed, without the need for highly sophisticated apparatus or skills. Moreover, their transference to industries and application of quality control could be easily performed. Serum albumin was adsorbed onto all types of tested nanoparticles. The saturation concentration was dependent on the nanoparticle size. In contrast, fibrinogen aggregation was dependent on nanoparticle surface charge. The complement activation was also influenced by the nanoparticle functionalization; the presence of a functionalizing agent increased complement activation, while the addition of an encapsulated compound only caused a slight increase. None of the nanoparticles influenced the coagulation cascade at low concentrations. However, at high concentrations, cationized nanoparticles did activate the coagulation cascade. Interactions of nanoparticles with erythrocytes did not reveal any hemolysis. Interactions of PLGA nanoparticles with blood proteins depended both on the nanoparticle properties and the protein studied. Independent of their loading/surface functionalization, PLGA nanoparticles did not influence the coagulation cascade and did not induce hemolysis of erythrocytes; they could be defined as safe concerning induction of embolization and cell lysis.

  11. P-I Snake Venom Metalloproteinase Is Able to Activate the Complement System by Direct Cleavage of Central Components of the Cascade

    Science.gov (United States)

    Pidde-Queiroz, Giselle; Magnoli, Fábio Carlos; Portaro, Fernanda C. V.; Serrano, Solange M. T.; Lopes, Aline Soriano; Paes Leme, Adriana Franco; van den Berg, Carmen W.; Tambourgi, Denise V.

    2013-01-01

    Background Snake Venom Metalloproteinases (SVMPs) are amongst the key enzymes that contribute to the high toxicity of snake venom. We have recently shown that snake venoms from the Bothrops genus activate the Complement system (C) by promoting direct cleavage of C-components and generating anaphylatoxins, thereby contributing to the pathology and spread of the venom. The aim of the present study was to isolate and characterize the C-activating protease from Bothrops pirajai venom. Results Using two gel-filtration chromatography steps, a metalloproteinase of 23 kDa that activates Complement was isolated from Bothrops pirajai venom. The mass spectrometric identification of this protein, named here as C-SVMP, revealed peptides that matched sequences from the P-I class of SVMPs. C-SVMP activated the alternative, classical and lectin C-pathways by cleaving the α-chain of C3, C4 and C5, thereby generating anaphylatoxins C3a, C4a and C5a. In vivo, C-SVMP induced consumption of murine complement components, most likely by activation of the pathways and/or by direct cleavage of C3, leading to a reduction of serum lytic activity. Conclusion We show here that a P-I metalloproteinase from Bothrops pirajai snake venom activated the Complement system by direct cleavage of the central C-components, i.e., C3, C4 and C5, thereby generating biologically active fragments, such as anaphylatoxins, and by cleaving the C1-Inhibitor, which may affect Complement activation control. These results suggest that direct complement activation by SVMPs may play a role in the progression of symptoms that follow envenomation. PMID:24205428

  12. Genetic Association of the Porcine C9 Complement Component with Hemolytic Complement Activity

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    D. V. A. Khoa

    2015-09-01

    Full Text Available The complement system is a part of the natural immune regulation mechanism against invading pathogens. Complement activation from three different pathways (classical, lectin, and alternative leads to the formation of C5-convertase, an enzyme for cleavage of C5 into C5a and C5b, followed by C6, C7, C8, and C9 in membrane attack complex. The C9 is the last complement component of the terminal lytic pathway, which plays an important role in lysis of the target cells depending on its self-polymerization to form transmembrane channels. To address the association of C9 with traits related to disease resistance, the complete porcine C9 cDNA was comparatively sequenced to detect single nucleotide polymorphisms (SNPs in pigs of the breeds Hampshire (HS, Duroc (DU, Berlin miniature pig (BMP, German Landrace (LR, Pietrain (PIE, and Muong Khuong (Vietnamese potbelly pig. Genotyping was performed in 417 F2 animals of a resource population (DUMI: DU×BMP that were vaccinated with Mycoplasma hyopneumoniae, Aujeszky diseases virus and porcine respiratory and reproductive syndrome virus at 6, 14 and 16 weeks of age, respectively. Two SNPs were detected within the third exon. One of them has an amino acid substitution. The European porcine breeds (LR and PIE show higher allele frequency of these SNPs than Vietnamese porcine breed (MK. Association of the substitution SNP with hemolytic complement activity indicated statistically significant differences between genotypes in the classical pathway but not in the alternative pathway. The interactions between eight time points of measurement of complement activity before and after vaccinations and genotypes were significantly different. The difference in hemolytic complement activity in the both pathways depends on genotype, kind of vaccine, age and the interaction to the other complement components. These results promote the porcine C9 (pC9 as a candidate gene to improve general animal health in the future.

  13. Surviving mousepox infection requires the complement system.

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    Elizabeth A Moulton

    2008-12-01

    Full Text Available Poxviruses subvert the host immune response by producing immunomodulatory proteins, including a complement regulatory protein. Ectromelia virus provides a mouse model for smallpox where the virus and the host's immune response have co-evolved. Using this model, our study investigated the role of the complement system during a poxvirus infection. By multiple inoculation routes, ectromelia virus caused increased mortality by 7 to 10 days post-infection in C57BL/6 mice that lack C3, the central component of the complement cascade. In C3(-/- mice, ectromelia virus disseminated earlier to target organs and generated higher peak titers compared to the congenic controls. Also, increased hepatic inflammation and necrosis correlated with these higher tissue titers and likely contributed to the morbidity in the C3(-/- mice. In vitro, the complement system in naïve C57BL/6 mouse sera neutralized ectromelia virus, primarily through the recognition of the virion by natural antibody and activation of the classical and alternative pathways. Sera deficient in classical or alternative pathway components or antibody had reduced ability to neutralize viral particles, which likely contributed to increased viral dissemination and disease severity in vivo. The increased mortality of C4(-/- or Factor B(-/- mice also indicates that these two pathways of complement activation are required for survival. In summary, the complement system acts in the first few minutes, hours, and days to control this poxviral infection until the adaptive immune response can react, and loss of this system results in lethal infection.

  14. Antibodies Against Complement Components: Relevance for the Antiphospholipid Syndrome-Biomarkers of the Disease and Biopharmaceuticals.

    Science.gov (United States)

    Bećarević, Mirjana

    2017-07-01

    Laboratory criterion for the diagnosis of antiphospholipid syndrome (APS) is the presence of antiphospholipid antibodies (aPL Abs). Complement system has a role in mediating aPL Abs-induced thrombosis in animal models. The importance of antibodies against complement components (potential biomarkers of APS) and the importance of antibodies with beneficial anti-complement effects in APS (as biopharmaceuticals) are reviewed. Antibodies against complement components described in APS patients, so far, are anti-C1q and anti-factor H Abs, although anti-factor B Abs and anti-C5a Abs were described in animal models of APS. Clinical studies in APS patients are limited to a small number of case reports. Studies that would confirm potential role of Abs against complement components (as potential biomarkers of APS) are lacking. Lack of randomized clinical trials (that would provide complete data for confirmation of beneficial effects of biopharmaceuticals in complement inhibition) in APS is alarming.

  15. Schizophrenia risk from complex variation of complement component 4

    DEFF Research Database (Denmark)

    Sekar, Aswin; Bialas, Allison R; de Rivera, Heather

    2016-01-01

    to identify. Here we show that this association arises in part from many structurally diverse alleles of the complement component 4 (C4) genes. We found that these alleles generated widely varying levels of C4A and C4B expression in the brain, with each common C4 allele associating with schizophrenia...

  16. Viral mimicry of the complement system

    Indian Academy of Sciences (India)

    The complement system is a potent innate immune mechanism consisting of cascades of proteins which are designed to fight against and annul intrusion of all the foreign pathogens. Although viruses are smaller in size and have relatively simple structure, they are not immune to complement attack. Thus, activation of the ...

  17. Complement System Part II: Role in Immunity

    Science.gov (United States)

    Merle, Nicolas S.; Noe, Remi; Halbwachs-Mecarelli, Lise; Fremeaux-Bacchi, Veronique; Roumenina, Lubka T.

    2015-01-01

    The complement system has been considered for a long time as a simple lytic cascade, aimed to kill bacteria infecting the host organism. Nowadays, this vision has changed and it is well accepted that complement is a complex innate immune surveillance system, playing a key role in host homeostasis, inflammation, and in the defense against pathogens. This review discusses recent advances in the understanding of the role of complement in physiology and pathology. It starts with a description of complement contribution to the normal physiology (homeostasis) of a healthy organism, including the silent clearance of apoptotic cells and maintenance of cell survival. In pathology, complement can be a friend or a foe. It acts as a friend in the defense against pathogens, by inducing opsonization and a direct killing by C5b–9 membrane attack complex and by triggering inflammatory responses with the anaphylatoxins C3a and C5a. Opsonization plays also a major role in the mounting of an adaptive immune response, involving antigen presenting cells, T-, and B-lymphocytes. Nevertheless, it can be also an enemy, when pathogens hijack complement regulators to protect themselves from the immune system. Inadequate complement activation becomes a disease cause, as in atypical hemolytic uremic syndrome, C3 glomerulopathies, and systemic lupus erythematosus. Age-related macular degeneration and cancer will be described as examples showing that complement contributes to a large variety of conditions, far exceeding the classical examples of diseases associated with complement deficiencies. Finally, we discuss complement as a therapeutic target. PMID:26074922

  18. Controlling the complement system in inflammation.

    Science.gov (United States)

    Kirschfink, M

    1997-12-01

    Inappropriate or excessive activation of the complement system can lead to harmful, potentially life-threatening consequences due to severe inflammatory tissue destruction. These consequences are clinically manifested in various disorders, including septic shock, multiple organ failure and hyperacute graft rejection. Genetic complement deficiencies or complement depletion have been proven to be beneficial in reducing tissue injury in a number of animal models of severe complement-dependent inflammation. It is therefore believed that therapeutic inhibition of complement is likely to arrest the process of certain diseases. Attempts to efficiently inhibit complement include the application of endogenous soluble complement inhibitors (C1-inhibitor, recombinant soluble complement receptor 1- rsCR1), the administration of antibodies, either blocking key proteins of the cascade reaction (e.g. C3, C5), neutralizing the action of the complement-derived anaphylatoxin C5a, or interfering with complement receptor 3 (CR3, CD18/11b)-mediated adhesion of inflammatory cells to the vascular endothelium. In addition, incorporation of membrane-bound complement regulators (DAF-CD55, MCP-CD46, CD59) has become possible by transfection of the correspondent cDNA into xenogeneic cells. Thereby, protection against complement-mediated inflammatory tissue damage could be achieved in various animal models of sepsis, myocardial as well as intestinal ischemia/reperfusion injury, adult respiratory distress syndrome, nephritis and graft rejection. Supported by results from first clinical trials, complement inhibition appears to be a suitable therapeutic approach to control inflammation. Current strategies to specifically inhibit complement in inflammation have been discussed at a recent meeting on the 'Immune Consequences of Trauma, Shock and Sepsis', held from March 4-8, 1997, in Munich, Germany. The Congress (chairman: E. Faist, Munich, Germany), which was held in close cooperation with various

  19. The role of the complement system in diabetic nephropathy

    DEFF Research Database (Denmark)

    Flyvbjerg, Allan

    2017-01-01

    -threatening disease. An increasing body of evidence points toward a role of the complement system in the pathogenesis of diabetic nephropathy. For example, circulating levels of mannose-binding lectin (MBL), a pattern recognition molecule of the innate immune system, have emerged as a robust biomarker...... for the development and progression of this disease, and evidence suggests that MBL, H-ficolin, complement component C3 and the membrane attack complex might contribute to renal injury in the hyperglycaemic mileu. New approaches to modulate the complement system might lead to the development of new agents to prevent...

  20. M. leprae components induce nerve damage by complement activation: identification of lipoarabinomannan as the dominant complement activator.

    Science.gov (United States)

    Bahia El Idrissi, Nawal; Das, Pranab K; Fluiter, Kees; Rosa, Patricia S; Vreijling, Jeroen; Troost, Dirk; Morgan, B Paul; Baas, Frank; Ramaglia, Valeria

    2015-05-01

    Peripheral nerve damage is the hallmark of leprosy pathology but its etiology is unclear. We previously identified the membrane attack complex (MAC) of the complement system as a key determinant of post-traumatic nerve damage and demonstrated that its inhibition is neuroprotective. Here, we determined the contribution of the MAC to nerve damage caused by Mycobacterium leprae and its components in mouse. Furthermore, we studied the association between MAC and the key M. leprae component lipoarabinomannan (LAM) in nerve biopsies of leprosy patients. Intraneural injections of M. leprae sonicate induced MAC deposition and pathological changes in the mouse nerve, whereas MAC inhibition preserved myelin and axons. Complement activation occurred mainly via the lectin pathway and the principal activator was LAM. In leprosy nerves, the extent of LAM and MAC immunoreactivity was robust and significantly higher in multibacillary compared to paucibacillary donors (p = 0.01 and p = 0.001, respectively), with a highly significant association between LAM and MAC in the diseased samples (r = 0.9601, p = 0.0001). Further, MAC co-localized with LAM on axons, pointing to a role for this M. leprae antigen in complement activation and nerve damage in leprosy. Our findings demonstrate that MAC contributes to nerve damage in a model of M. leprae-induced nerve injury and its inhibition is neuroprotective. In addition, our data identified LAM as the key pathogen associated molecule that activates complement and causes nerve damage. Taken together our data imply an important role of complement in nerve damage in leprosy and may inform the development of novel therapeutics for patients.

  1. Hepatitis C virus NS3/4A protease inhibits complement activation by cleaving complement component 4.

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    Seiichi Mawatari

    Full Text Available BACKGROUND: It has been hypothesized that persistent hepatitis C virus (HCV infection is mediated in part by viral proteins that abrogate the host immune response, including the complement system, but the precise mechanisms are not well understood. We investigated whether HCV proteins are involved in the fragmentation of complement component 4 (C4, composed of subunits C4α, C4β, and C4γ, and the role of HCV proteins in complement activation. METHODS: Human C4 was incubated with HCV nonstructural (NS 3/4A protease, core, or NS5. Samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then subjected to peptide sequencing. The activity of the classical complement pathway was examined using an erythrocyte hemolysis assay. The cleavage pattern of C4 in NS3/4A-expressing and HCV-infected cells, respectively, was also examined. RESULTS: HCV NS3/4A protease cleaved C4γ in a concentration-dependent manner, but viral core and NS5 did not. A specific inhibitor of NS3/4A protease reduced C4γ cleavage. NS3/4A protease-mediated cleavage of C4 inhibited classical pathway activation, which was abrogated by a NS3/4A protease inhibitor. In addition, co-transfection of cells with C4 and wild-type NS3/4A, but not a catalytic-site mutant of NS3/4A, produced cleaved C4γ fragments. Such C4 processing, with a concomitant reduction in levels of full-length C4γ, was also observed in HCV-infected cells expressing C4. CONCLUSIONS: C4 is a novel cellular substrate of the HCV NS3/4A protease. Understanding disturbances in the complement system mediated by NS3/4A protease may provide new insights into the mechanisms underlying persistent HCV infection.

  2. Francisella tularensis Confronts the Complement System

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    Susan R. Brock

    2017-12-01

    Full Text Available Francisella tularensis has developed a number of effective evasion strategies to counteract host immune defenses, not the least of which is its ability to interact with the complement system to its own advantage. Following exposure of the bacterium to fresh human serum, complement is activated and C3b and iC3b can be found covalently attached to the bacterial surface. However, the lipopolysaccharide and capsule of the F. tularensis cell wall prevent complement-mediated lysis and endow the bacterium with serum resistance. Opsonization of F. tularensis with C3 greatly increases its uptake by human neutrophils, dendritic cells and macrophages. Uptake occurs by an unusual looping morphology in human macrophages. Complement receptor 3 is thought to play an important role in opsonophagocytosis by human macrophages, and signaling through this receptor can antagonize Toll-like receptor 2-initiated macrophage activation. Complement C3 also determines the survival of infected human macrophages and perhaps other cell types. C3-opsonization of F. tularensis subsp. tularensis strain SCHU S4 results in greatly increased death of infected human macrophages, which requires more than complement receptor engagement and is independent of the intracellular replication by the pathogen. Given its entry into the cytosol of host cells, F. tularensis has the potential for a number of other complement-mediated interactions. Studies on the uptake C3-opsonized adenovirus have suggested the existence of a C3 sensing system that initiates cellular responses to cytosolic C3b present on invading microbes. Here we propose that C3 peptides enter the cytosol of human macrophages following phagosome escape of F. tularensis and are recognized as intruding molecular patterns that signal host cell death. With the discovery of new roles for intracellular C3, a better understanding of tularemia pathogenesis is likely to emerge.

  3. IrC2/Bf - A yeast and Borrelia responsive component of the complement system from the hard tick Ixodes ricinus

    Czech Academy of Sciences Publication Activity Database

    Urbanová, V.; Hajdušek, O.; Šíma, R.; Franta, Z.; Hönig Mondeková, Helena; Grunclová, L.; Bartošová-Sojková, P.; Jalovecká, M.; Kopáček, P.

    2018-01-01

    Roč. 76, FEB 2018 (2018), s. 86-94 ISSN 0145-305X Institutional support: RVO:61388971 Keywords : Borrelia * C3-complement convertase * Factor B Subject RIV: EE - Microbiology, Virology OBOR OECD: Microbiology Impact factor: 3.218, year: 2016

  4. Assembly and activation of alternative complement components on endothelial cell-anchored ultra-large von Willebrand factor links complement and hemostasis-thrombosis.

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    Nancy A Turner

    Full Text Available Vascular endothelial cells (ECs express and release protein components of the complement pathways, as well as secreting and anchoring ultra-large von Willebrand factor (ULVWF multimers in long string-like structures that initiate platelet adhesion during hemostasis and thrombosis. The alternative complement pathway (AP is an important non-antibody-requiring host defense system. Thrombotic microangiopathies can be associated with defective regulation of the AP (atypical hemolytic-uremic syndrome or with inadequate cleavage by ADAMTS-13 of ULVWF multimeric strings secreted by/anchored to ECs (thrombotic thrombocytopenic purpura. Our goal was to determine if EC-anchored ULVWF strings caused the assembly and activation of AP components, thereby linking two essential defense mechanisms.We quantified gene expression of these complement components in cultured human umbilical vein endothelial cells (HUVECs by real-time PCR: C3 and C5; complement factor (CF B, CFD, CFP, CFH and CFI of the AP; and C4 of the classical and lectin (but not alternative complement pathways. We used fluorescent microscopy, monospecific antibodies against complement components, fluorescent secondary antibodies, and the analysis of >150 images to quantify the attachment of HUVEC-released complement proteins to ULVWF strings secreted by, and anchored to, the HUVECs (under conditions of ADAMTS-13 inhibition. We found that HUVEC-released C4 did not attach to ULVWF strings, ruling out activation of the classical and lectin pathways by the strings. In contrast, C3, FB, FD, FP and C5, FH and FI attached to ULVWF strings in quantitative patterns consistent with assembly of the AP components into active complexes. This was verified when non-functional FB blocked the formation of AP C3 convertase complexes (C3bBb on ULVWF strings.AP components are assembled and activated on EC-secreted/anchored ULVWF multimeric strings. Our findings provide one possible molecular mechanism for clinical

  5. Micrurus snake venoms activate human complement system and generate anaphylatoxins

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    Tanaka Gabriela D

    2012-01-01

    Full Text Available Abstract Background The genus Micrurus, coral snakes (Serpentes, Elapidae, comprises more than 120 species and subspecies distributed from the south United States to the south of South America. Micrurus snake bites can cause death by muscle paralysis and further respiratory arrest within a few hours after envenomation. Clinical observations show mainly neurotoxic symptoms, although other biological activities have also been experimentally observed, including cardiotoxicity, hemolysis, edema and myotoxicity. Results In the present study we have investigated the action of venoms from seven species of snakes from the genus Micrurus on the complement system in in vitro studies. Several of the Micrurus species could consume the classical and/or the lectin pathways, but not the alternative pathway, and C3a, C4a and C5a were generated in sera treated with the venoms as result of this complement activation. Micrurus venoms were also able to directly cleave the α chain of the component C3, but not of the C4, which was inhibited by 1,10 Phenanthroline, suggesting the presence of a C3α chain specific metalloprotease in Micrurus spp venoms. Furthermore, complement activation was in part associated with the cleavage of C1-Inhibitor by protease(s present in the venoms, which disrupts complement activation control. Conclusion Micrurus venoms can activate the complement system, generating a significant amount of anaphylatoxins, which may assist due to their vasodilatory effects, to enhance the spreading of other venom components during the envenomation process.

  6. In Vitro Immunologic Studies with an {sup 131}I-Labelled Component of Complement

    Energy Technology Data Exchange (ETDEWEB)

    Spar, I. L.; Benz, L. L. [Department of Radiation Biology and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY (United States)

    1970-02-15

    Most of the in vitro immunological studies using radioactive isotopes have involved labelling of the reacting antigen or antibody. For meaningful results, it is also necessary to use a relatively purified preparation of either the antigen or antibody. This has been difficult to obtain when the antigenic component is ill- defined, as in tissue or tumour immunity. It seemed possible that detection of reactions of this type could be done more easily by using labelled components of haemolytic complement, since some of the factors involved in this 9-11 factor system are known to firmly bind to most antigen-antibody combinations. As an initial step in this study, {sup 125}I- or {sup 131}I-labelled components of complement were used to detect and quantitate known antigen-antibody systems. The initial reacting component of guinea pig complement, C'l, was partially purified and labelled with {sup 125}I or {sup 131}I. It was found that labelled C'l would react with ovalbumin- antiovalbumin (OA) precipitates and would bind to sensitized sheep cells (EA) in proportion to the amount of haemolysin bound to the cells. EDTA elusion of such bound {sup 125}I-C'l yielded a product that would react with EA or OA to a much greater extent than the starting material. In addition, lysis of EA would occur after binding of eluted {sup 125}I-C'l if the remaining complement components were added to the system. In further studies it was found that {sup 131}I-C'l could be used to detect reactions between anti-kidney antisera and hpmogenates of kidney, liver and lung. Extension of this work with isotopically labelled components of complement to a study involving tissue sections after incubation with antisera could lead to defection of tissue-antitissue antibody binding in situ. By utilizing autoradiographic techniques, one can further extend this system to define the site of antibody fixation. A distinct advantage of this approach is that the isotopically labelled reactant, complement, is

  7. The Emerging Role of Complement Lectin Pathway in Trypanosomatids: Molecular Bases in Activation, Genetic Deficiencies, Susceptibility to Infection, and Complement System-Based Therapeutics

    Directory of Open Access Journals (Sweden)

    Ingrid Evans-Osses

    2013-01-01

    Full Text Available The innate immune system is evolutionary and ancient and is the pivotal line of the host defense system to protect against invading pathogens and abnormal self-derived components. Cellular and molecular components are involved in recognition and effector mechanisms for a successful innate immune response. The complement lectin pathway (CLP was discovered in 1990. These new components at the complement world are very efficient. Mannan-binding lectin (MBL and ficolin not only recognize many molecular patterns of pathogens rapidly to activate complement but also display several strategies to evade innate immunity. Many studies have shown a relation between the deficit of complement factors and susceptibility to infection. The recently discovered CLP was shown to be important in host defense against protozoan microbes. Although the recognition of pathogen-associated molecular patterns by MBL and Ficolins reveal efficient complement activations, an increase in deficiency of complement factors and diversity of parasite strategies of immune evasion demonstrate the unsuccessful effort to control the infection. In the present paper, we will discuss basic aspects of complement activation, the structure of the lectin pathway components, genetic deficiency of complement factors, and new therapeutic opportunities to target the complement system to control infection.

  8. Characterization and expression analysis of a complement component gene in sea cucumber ( Apostichopus japonicus)

    Science.gov (United States)

    Chen, Zhong; Zhou, Zunchun; Yang, Aifu; Dong, Ying; Guan, Xiaoyan; Jiang, Bei; Wang, Bai

    2015-12-01

    The complement system plays a crucial role in the innate immune system of animals. It can be activated by distinct yet overlapping classical, alternative and lectin pathways. In the alternative pathway, complement factor B (Bf) serves as the catalytic subunit of complement component 3 (C3) convertase, which plays the central role among three activation pathways. In this study, the Bf gene in sea cucumber ( Apostichopus japonicus), termed AjBf, was obtained by rapid amplification of cDNA ends (RACE). The full-length cDNA of AjBf was 3231 bp in length barring the poly (A) tail. It contained an open reading frame (ORF) of 2742 bp encoding 913 amino acids, a 105 bp 5'-UTR (5'-terminal untranslated region) and a 384 bp 3'-UTR. AjBf was a mosaic protein with six CCP (complement control protein) domains, a VWA (von Willebrand factor A) domain, and a serine protease domain. The deduced molecular weight of AjBf protein was 101 kDa. Quantitative real time PCR (qRT-PCR) analysis indicated that the expression level of AjBf in A. japonicus was obviously higher at larval stage than that at embryonic stage. Expression detection in different tissues showed that AjBf expressed higher in coelomocytes than in other four tissues. In addation, AjBf expression in different tissues was induced significantly after LPS or PolyI:C challenge. These results indicated that AjBf plays an important role in immune responses to pathogen infection.

  9. Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis

    Science.gov (United States)

    Woehrl, Bianca; Brouwer, Matthijs C.; Murr, Carmen; Heckenberg, Sebastiaan G.B.; Baas, Frank; Pfister, Hans W.; Zwinderman, Aeilko H.; Morgan, B. Paul; Barnum, Scott R.; van der Ende, Arie; Koedel, Uwe; van de Beek, Diederik

    2011-01-01

    Pneumococcal meningitis is the most common and severe form of bacterial meningitis. Fatality rates are substantial, and long-term sequelae develop in about half of survivors. Disease outcome has been related to the severity of the proinflammatory response in the subarachnoid space. The complement system, which mediates key inflammatory processes, has been implicated as a modulator of pneumococcal meningitis disease severity in animal studies. Additionally, SNPs in genes encoding complement pathway proteins have been linked to susceptibility to pneumococcal infection, although no associations with disease severity or outcome have been established. Here, we have performed a robust prospective nationwide genetic association study in patients with bacterial meningitis and found that a common nonsynonymous complement component 5 (C5) SNP (rs17611) is associated with unfavorable disease outcome. C5 fragment levels in cerebrospinal fluid (CSF) of patients with bacterial meningitis correlated with several clinical indicators of poor prognosis. Consistent with these human data, C5a receptor–deficient mice with pneumococcal meningitis had lower CSF wbc counts and decreased brain damage compared with WT mice. Adjuvant treatment with C5-specific monoclonal antibodies prevented death in all mice with pneumococcal meningitis. Thus, our results suggest C5-specific monoclonal antibodies could be a promising new antiinflammatory adjuvant therapy for pneumococcal meningitis. PMID:21926466

  10. The complement system and its role in the pathogenesis of periodontitis

    DEFF Research Database (Denmark)

    Damgaard, Christian; Holmstrup, Palle; Van Dyke, Thomas E.

    2015-01-01

    Periodontitis is a highly prevalent inflammatory disease in tooth supporting tissues, induced by bacteria growing in a biofilm on tooth surfaces. Components of the complement system are present in the periodontal tissue and the system is activated in periodontitis. Continuous complement activation...... and modulation by bacteria within the biofilm in periodontal pockets, however, may enhance local tissue destruction, providing the biofilm with both essential nutrients and space to grow. A more profound understanding of the mechanisms involved in complement-derived tissue degradation may facilitate...... with an emphasis on interaction of complement with bacteria from periodontitis-associated biofilm....

  11. Complement components of nerve regeneration conditioned fluid influence the microenvironment of nerve regeneration

    Directory of Open Access Journals (Sweden)

    Guang-shuai Li

    2016-01-01

    Full Text Available Nerve regeneration conditioned fluid is secreted by nerve stumps inside a nerve regeneration chamber. A better understanding of the proteinogram of nerve regeneration conditioned fluid can provide evidence for studying the role of the microenvironment in peripheral nerve regeneration. In this study, we used cylindrical silicone tubes as the nerve regeneration chamber model for the repair of injured rat sciatic nerve. Isobaric tags for relative and absolute quantitation proteomics technology and western blot analysis confirmed that there were more than 10 complement components (complement factor I, C1q-A, C1q-B, C2, C3, C4, C5, C7, C8ß and complement factor D in the nerve regeneration conditioned fluid and each varied at different time points. These findings suggest that all these complement components have a functional role in nerve regeneration.

  12. A novel polymorphism of human complement component C3 detected by means of a monoclonal antibody

    DEFF Research Database (Denmark)

    Koch, C; Behrendt, N

    1986-01-01

    A mouse monoclonal antibody, HAV 4-1, obtained after immunization of a BALB/c mouse with purified C3F, detected a novel genetic polymorphism of human complement component C3 in a simple immunoblotting system. The frequency of HAV 4-1-positive genes was 20.1%. Reactivity of HAV 4-1 was closely...... related to C3F, but certain individuals with the C3F allele did not react with HAV 4-1. Conversely, certain C3S homozygous individuals did react with HAV 4-1. The polymorphism detected by this monoclonal antibody is therefore different from the previously described polymorphism based on charge differences....

  13. Interactions of the humoral pattern recognition molecule PTX3 with the complement system

    DEFF Research Database (Denmark)

    Doni, Andrea; Garlanda, Cecilia; Bottazzi, Barbara

    2012-01-01

    The innate immune system comprises a cellular and a humoral arm. The long pentraxin PTX3 is a fluid phase pattern recognition molecule, which acts as an essential component of the humoral arm of innate immunity. PTX3 has antibody-like properties including interactions with complement components....... PTX3 interacts with C1q, ficolin-1 and ficolin-2 as well as mannose-binding lectin, recognition molecules in the classical and lectin complement pathways. The formation of these heterocomplexes results in cooperative pathogen recognition and complement activation. Interactions with C4b binding protein...

  14. Systemic complement activation in age-related macular degeneration.

    Directory of Open Access Journals (Sweden)

    Hendrik P N Scholl

    Full Text Available Dysregulation of the alternative pathway (AP of complement cascade has been implicated in the pathogenesis of age-related macular degeneration (AMD, the leading cause of blindness in the elderly. To further test the hypothesis that defective control of complement activation underlies AMD, parameters of complement activation in blood plasma were determined together with disease-associated genetic markers in AMD patients. Plasma concentrations of activation products C3d, Ba, C3a, C5a, SC5b-9, substrate proteins C3, C4, factor B and regulators factor H and factor D were quantified in patients (n = 112 and controls (n = 67. Subjects were analyzed for single nucleotide polymorphisms in factor H (CFH, factor B-C2 (BF-C2 and complement C3 (C3 genes which were previously found to be associated with AMD. All activation products, especially markers of chronic complement activation Ba and C3d (p<0.001, were significantly elevated in AMD patients compared to controls. Similar alterations were observed in factor D, but not in C3, C4 or factor H. Logistic regression analysis revealed better discriminative accuracy of a model that is based only on complement activation markers Ba, C3d and factor D compared to a model based on genetic markers of the complement system within our study population. In both the controls' and AMD patients' group, the protein markers of complement activation were correlated with CFH haplotypes.This study is the first to show systemic complement activation in AMD patients. This suggests that AMD is a systemic disease with local disease manifestation at the ageing macula. Furthermore, the data provide evidence for an association of systemic activation of the alternative complement pathway with genetic variants of CFH that were previously linked to AMD susceptibility.

  15. Effects of radiographic contrast media on the serum complement system

    International Nuclear Information System (INIS)

    Tirone, P.; Boldrini, E.

    1983-01-01

    The authors explored the activation of the complement system produced by a nonionic organic iodine compound, namely iopamidol, which is proposed as a contrast medium for radiographic examination by intravenous and intra-arterial injection. The study was conducted in vitro versus established ionic contrasts (diatrizoate, iothalamate, acetrizoate) and a nonionic compound (metrizamide). The adopted experimental model was the immunohemolytic detector system, in which the immune complex consisted of goat erythrocytes sensitized with the corresponding antibody (hemolysin), and complement (C') was supplied by guinea pig serum. All the products caused complement activation. The results show that nonionic contrast media produce less activation of the complement system than the traditional ionic contrast. Thus the use of nonionic contrast for radiological procedures necessitating the introduction of contrast material into the blood compartment would imply a reduced risk of anaphylactoid reactions. (orig.)

  16. Complement: a key system for immune surveillance and homeostasis.

    Science.gov (United States)

    Ricklin, Daniel; Hajishengallis, George; Yang, Kun; Lambris, John D

    2010-09-01

    Nearly a century after the significance of the human complement system was recognized, we have come to realize that its functions extend far beyond the elimination of microbes. Complement acts as a rapid and efficient immune surveillance system that has distinct effects on healthy and altered host cells and foreign intruders. By eliminating cellular debris and infectious microbes, orchestrating immune responses and sending 'danger' signals, complement contributes substantially to homeostasis, but it can also take action against healthy cells if not properly controlled. This review describes our updated view of the function, structure and dynamics of the complement network, highlights its interconnection with immunity at large and with other endogenous pathways, and illustrates its multiple roles in homeostasis and disease.

  17. The intestinal complement system in inflammatory bowel disease: Shaping intestinal barrier function.

    Science.gov (United States)

    Sina, Christian; Kemper, Claudia; Derer, Stefanie

    2018-06-01

    The complement system is part of innate sensor and effector systems such as the Toll-like receptors (TLRs). It recognizes and quickly systemically and/or locally respond to microbial-associated molecular patterns (MAMPs) with a tailored defense reaction. MAMP recognition by intestinal epithelial cells (IECs) and appropriate immune responses are of major importance for the maintenance of intestinal barrier function. Enterocytes highly express various complement components that are suggested to be pivotal for proper IEC function. Appropriate activation of the intestinal complement system seems to play an important role in the resolution of chronic intestinal inflammation, while over-activation and/or dysregulation may worsen intestinal inflammation. Mice deficient for single complement components suffer from enhanced intestinal inflammation mimicking the phenotype of patients with chronic inflammatory bowel disease (IBD) such as Crohn's disease (CD) or ulcerative colitis (UC). However, the mechanisms leading to complement expression in IECs seem to differ markedly between UC and CD patients. Hence, how IECs, intestinal bacteria and epithelial cell expressed complement components interact in the course of IBD still remains to be mostly elucidated to define potential unique patterns contributing to the distinct subtypes of intestinal inflammation observed in CD and UC. Copyright © 2018 Elsevier Ltd. All rights reserved.

  18. The complement system at the embryo implantation site: friend or foe?

    Directory of Open Access Journals (Sweden)

    Roberta eBulla

    2012-03-01

    Full Text Available An inflammatory-like process and vascular remodeling represent the main changes that occur in decidua in the early phase of pregnancy. These changes are partly induced by trophoblast cells that colonize the decidua and are also contributed by the complement system. C1q is one of the component components produced at feto-maternal interface that serves an important function in placental development. Decidual endothelial cells synthesize and express C1q on the cell surface where it acts as a molecular bridge between endovascular trophoblast and endothelial cells. C1q is also produced by extravillous trophoblast and is used to favor trophoblast migration through the decidua. C7 is another component produced and expressed on the membrane of endothelial cells and is involved in the control of the proinflammatory effect of the terminal complement complex. Defective expression of C1q by trophoblast is associated with impaired trophoblast invasion of decidua and may have important implications in pregnancy disorders such as preeclampsia characterized by reduced vascular remodeling. Local control of complement activation by several complement regulators including cell-bound C7 is critical to prevent complement-mediated tissue damage as suggested by recent data showing an association of preeclampsia with mutations in the genes encoding for some complement regulators.

  19. Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival.

    Science.gov (United States)

    Caine, Jennifer A; Lin, Yi-Pin; Kessler, Julie R; Sato, Hiromi; Leong, John M; Coburn, Jenifer

    2017-12-01

    Borrelia burgdorferi (Bb) is the causative agent of Lyme disease in the United States, a disease that can result in carditis, and chronic and debilitating arthritis and/or neurologic symptoms if left untreated. Bb survives in the midgut of the Ixodes scapularis tick, or within tissues of immunocompetent hosts. In the early stages of infection, the bacteria are present in the bloodstream where they must resist clearance by the innate immune system of the host. We have found a novel role for outer surface protein C (OspC) from B. burgdorferi and B. garinii in interactions with the complement component C4b and bloodstream survival in vivo. Our data show that OspC inhibits the classical and lectin complement pathways and competes with complement protein C2 for C4b binding. Resistance to complement is important for maintenance of the lifecycle of Bb, enabling survival of the pathogen within the host as well as in the midgut of a feeding tick when ospC expression is induced. © 2017 John Wiley & Sons Ltd.

  20. Breaking down the complement system: a review and update on novel therapies.

    Science.gov (United States)

    Reddy, Yuvaram N V; Siedlecki, Andrew M; Francis, Jean M

    2017-03-01

    The complement system represents one of the more primitive forms of innate immunity. It has increasingly been found to contribute to pathologies in the native and transplanted kidney. We provide a concise review of the physiology of the complement cascade, and discuss current and upcoming complement-based therapies. Current agents in clinical use either bind to complement components directly or prevent complement from binding to antibodies affixed to the endothelial surface. These include C1 esterase inhibitors, anti-C5 mAbs, anti-CD20 mAbs, and proteasome inhibitors. Treatment continues to show efficacy in the atypical hemolytic uremic syndrome and antibody-mediated rejection. Promising agents not currently available include CCX168, TP10, AMY-101, factor D inhibitors, coversin, and compstatin. Several new trials are targeting complement inhibition to treat antineutrophilic cystoplasmic antibody (ANCA)-associated vasculitis, C3 glomerulopathy, thrombotic microangiopathy, and IgA nephropathy. New agents for the treatment of the atypical hemolytic uremic syndrome are also in development. Complement-based therapies are being considered for targeted therapy in the atypical hemolytic uremic syndrome and antibody-mediated rejection, C3 glomerulopathy, and ANCA-associated vasculitis. A few agents are currently in use as orphan drugs. A number of other drugs are in clinical trials and, overall, are showing promising preliminary results.

  1. Characterization of the third component of complement (C3) after activation by cigarette smoke

    International Nuclear Information System (INIS)

    Kew, R.R.; Ghebrehiwet, B.; Janoff, A.

    1987-01-01

    Activation of lung complement by tobacco smoke may be an important pathogenetic factor in the development of pulmonary emphysema in smokers. We previously showed that cigarette smoke can modify C3 and activate the alternative pathway of complement in vitro. However, the mechanism of C3 activation was not fully delineated in these earlier studies. In the present report, we show that smoke-treated C3 induces cleavage of the alternative pathway protein, Factor B, when added to serum containing Mg-EGTA. This effect of cigarette smoke is specific for C3 since smoke-treated C4, when added to Mg-EGTA-treated serum, fails to activate the alternative pathway and fails to induce Factor B cleavage. Smoke-modified C3 no longer binds significant amounts of [ 14 C]methylamine (as does native C3), and relatively little [ 14 C]methylamine is incorporated into its alpha-chain. Thus, prior internal thiolester bond cleavage appears to have occurred in C3 activated by cigarette smoke. Cigarette smoke components also induce formation of noncovalently associated, soluble C3 multimers, with a Mr ranging from 1 to 10 million. However, prior cleavage of the thiolester bond in C3 with methylamine prevents the subsequent formation of these smoke-induced aggregates. These data indicate that cigarette smoke activates the alternative pathway of complement by specifically modifying C3 and that these modifications include cleavage of the thiolester bond in C3 and formation of noncovalently linked C3 multimers

  2. Complement System in the Pathogenesis of Benign Lymphoepithelial Lesions of the Lacrimal Gland.

    Directory of Open Access Journals (Sweden)

    Jing Li

    Full Text Available We aimed to examine the potential involvement of local complement system gene expression in the pathogenesis of benign lymphoepithelial lesions (BLEL of the lacrimal gland.We collected data from 9 consecutive pathologically confirmed patients with BLEL of the lacrimal gland and 9 cases with orbital cavernous hemangioma as a control group, and adopted whole genome microarray to screen complement system-related differential genes, followed by RT-PCR verification and in-depth enrichment analysis (Gene Ontology analysis of the gene sets.The expression of 14 complement system-related genes in the pathologic tissue, including C2, C3, ITGB2, CR2, C1QB, CR1, ITGAX, CFP, C1QA, C4B|C4A, FANCA, C1QC, C3AR1 and CFHR4, were significantly upregulated while 7 other complement system-related genes, C5, CFI, CFHR1|CFH, CFH, CD55, CR1L and CFD were significantly downregulated in the lacrimal glands of BLEL patients. The microarray results were consistent with RT-PCR analysis results. Immunohistochemistry analysis of C3c and C1q complement component proteins in the resected tissue were positive in BLEL patients, while the control group had negative expression of these proteins. Gene ontology (GO analysis revealed that activation of the genes of complement system-mediated signaling pathways were the most enriched differential gene group in BLEL patients.Local expression of complement components is prominently abnormal in BLEL, and may well play a role in its pathogenesis.

  3. Monotest in the complement fixation test: the Chorus system

    Directory of Open Access Journals (Sweden)

    Laura Meli

    2009-06-01

    Full Text Available The complement fixation test (CFT is a method used for the detection of antibodies against pathogens of infectious diseases, it has been proved to be a useful diagnostic method in the detection of acute disease in many medical laboratories.The test performed manually is time consuming and needs very skilled personnel.This study evaluates the automated Chorus CFT system with 87 serum samples in comparison with manual method using Virion-Serion reagents, against a panel of antigens, such as Adenovirus, Influenza A and B virus, Respiratory Syncythial Virus, Parainfluenza Mix, Mycoplasma Pneumoniae, and Echinococcus. The Chorus system includes standardized reagents and a monotest device to perform the single assay. In comparison to the manual CFT method, the correlation is 91.6% (7/83.The results obtained show that the automated Chorus system can be applied for detecting complement fixation antibodies against different infectious disease agents.

  4. Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis.

    Science.gov (United States)

    Eriksson, Charlotta E; Studahl, Marie; Bergström, Tomas

    2016-06-15

    Herpes simplex encephalitis (HSE) is characterized by a pronounced inflammatory activity in the central nervous system (CNS). Here, we investigated the acute and prolonged complement system activity in HSE patients, by using enzyme-linked immunosorbent assays (ELISAs) for numerous complement components (C). We found increased cerebrospinal fluid concentrations of C3a, C3b, C5 and C5a in HSE patients compared with healthy controls. C3a and C5a concentrations remained increased also compared with patient controls. Our results conclude that the complement system is activated in CNS during HSE in the acute phase, and interestingly also in later stages supporting previous reports of prolonged inflammation. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. Induction of passive Heymann nephritis in complement component 6-deficient PVG rats.

    Science.gov (United States)

    Spicer, S Timothy; Tran, Giang T; Killingsworth, Murray C; Carter, Nicole; Power, David A; Paizis, Kathy; Boyd, Rochelle; Hodgkinson, Suzanne J; Hall, Bruce M

    2007-07-01

    Passive Heymann nephritis (PHN), a model of human membranous nephritis, is induced in susceptible rat strains by injection of heterologous antisera to rat renal tubular Ag extract. PHN is currently considered the archetypal complement-dependent form of nephritis, with the proteinuria resulting from sublytic glomerular epithelial cell injury induced by the complement membrane attack complex (MAC) of C5b-9. This study examined whether C6 and MAC are essential to the development of proteinuria in PHN by comparing the effect of injection of anti-Fx1A antisera into PVG rats deficient in C6 (PVG/C6(-)) and normal PVG rats (PVG/c). PVG/c and PVG/C6(-) rats developed similar levels of proteinuria at 3, 7, 14, and 28 days following injection of antisera. Isolated whole glomeruli showed similar deposition of rat Ig and C3 staining in PVG/c and PVG/C6(-) rats. C9 deposition was abundant in PVG/c but was not detected in PVG/C6(-) glomeruli, indicating C5b-9/MAC had not formed in PVG/C6(-) rats. There was also no difference in the glomerular cellular infiltrate of T cells and macrophages nor the size of glomerular basement membrane deposits measured on electron micrographs. To examine whether T cells effect injury, rats were depleted of CD8+ T cells which did not affect proteinuria in the early heterologous phase but prevented the increase in proteinuria associated with the later autologous phase. These studies showed proteinuria in PHN occurs without MAC and that other mechanisms, such as immune complex size, early complement components, CD4+ and CD8+ T cells, disrupt glomerular integrity and lead to proteinuria.

  6. Molecular basis for genetic deficiency of the second component of human complement

    International Nuclear Information System (INIS)

    Cole, F.S.; Whitehead, A.S.; Auerbach, H.S.; Lint, T.; Zeitz, H.J.; Kilbridge, P.; Colten, H.R.

    1985-01-01

    Genetic deficiency of the second component of complement (C2) is the most common complement-deficiency state among Western Europeans and is frequently associated with autoimmune diseases. To examine the molecular basis of this deficiency, the authors established cultures of blood monocytes from four families with C2-deficient members. Using a hemolytic-plaque assay, [ 35 S]methionine metabolic labeling of proteins in tissue culture and immunoprecipitation, RNA extraction and Northern blot analysis, and DNA restriction-enzyme digestion and Southern blot analysis, the authors found that C2 deficiency is not due to a major gene deletion or rearrangement but is the result of a specific and selective pretranslational regulatory defect in C2 gene expression. This leads to a lack of detectable C2 mRNA and a lack of synthesis of C2 protein. The approach used in this study should prove useful in examination of other plasma protein deficiencies, especially those in which the deficient gene is normally expressed in peripheral-blood monocytes or tissue macrophages and in which ethical considerations preclude the use of liver or other tissue for study

  7. 2-component heating systems

    Energy Technology Data Exchange (ETDEWEB)

    Radtke, W

    1987-03-01

    The knowledge accumulated only recently of the damage to buildings and the hazards of formaldehyde, radon and hydrocarbons has been inducing louder calls for ventilation, which, on their part, account for the fact that increasing importance is being attached to the controlled ventilation of buildings. Two-component heating systems provide for fresh air and thermal comfort in one. While the first component uses fresh air blown directly and controllably into the rooms, the second component is similar to the Roman hypocaustic heating systems, meaning that heated outer air is circulating under the floor, thus providing for hot surfaces and thermal comfort. Details concerning the two-component heating system are presented along with systems diagrams, diagrams of the heating system and tables identifying the respective costs. Descriptions are given of the two systems components, the fast heat-up, the two-component made, the change of air, heat recovery and control systems. Comparative evaluations determine the differences between two-component heating systems and other heating systems. Conclusive remarks are dedicated to energy conservation and comparative evaluations of costs. (HWJ).

  8. Therapeutic inhibition of the complement system. Y2K update.

    Science.gov (United States)

    Asghar, S S; Pasch, M C

    2000-09-01

    -transplantation in clinical practice a reality. Several recombinant variants of complement receptor 1 (CR1) have been produced. The most effective of these appears to be sCR1-SLe x, sCR1 part of which inhibits complement and carbohydrate Sle x moiety inhibits selectin mediated interactions of neutrophils and lymphocytes with endothelium. Although clinical trials of sCR1 in humans is eagerly awaited, several of the recombinant versions of sCR1 have been shown to suppress ischemia/reperfusion injury, thermal trauma, and immune complex mediated inflammation. They have also been shown to be effective in experimental models of systemic sclerosis, arthritis, myasthenia gravis, Guillain Barré syndrome and glomerulonephritis. Intravenous immunoglobulin, three of the most prominent properties of which are neutralization of autoantibody activity, suppression of autoantibody production and inhibition of complement activity, is being used in several diseases. These include autoimmune thrombocyopenic purpura, Kawasaki disease and several neurological diseases such as myasthenia gravis and Guillain Barre syndrome. In many uncontrolled small scale studies intravenous immunoglobulin has been shown to be effective in many immunological including dermatological diseases; controlled clinical trials in a large number of patients with these diseases is needed to establish the efficacy. It is hoped that in future therapeutic inhibition of complement will be one of the major approaches to combat many human diseases.

  9. CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a

    Directory of Open Access Journals (Sweden)

    Rachel Vaivoda

    2015-01-01

    Full Text Available CYP4Fs were first identified as enzymes that catalyze hydroxylation of leukotriene B4 (LTB4. CYP4F18 has an unusual expression in neutrophils and was predicted to play a role in regulating LTB4-dependent inflammation. We compared chemotaxis of wild-type and Cyp4f18 knockout neutrophils using an in vitro assay. There was no significant difference in the chemotactic response to LTB4, but the response to complement component C5a increased 1.9–2.25-fold in knockout cells compared to wild-type (P < 0.01. This increase was still observed when neutrophils were treated with inhibitors of eicosanoid synthesis. There were no changes in expression of other CYP4 enzymes in knockout neutrophils that might compensate for loss of CYP4F18 or lead to differences in activity. A mouse model of dextran sodium sulfate colitis was used to investigate the consequences of increased C5a-dependent chemotaxis in vivo, but there was no significant difference in weight loss, disease activity, or colonic tissue myeloperoxidase between wild-type and Cyp4f18 knockout mice. This study demonstrates the limitations of inferring CYP4F function based on an ability to use LTB4 as a substrate, points to expanding roles for CYP4F enzymes in immune regulation, and underscores the in vivo challenges of CYP knockout studies.

  10. Pasteurella pneumotropica evades the human complement system by acquisition of the complement regulators factor H and C4BP.

    Directory of Open Access Journals (Sweden)

    Alfredo Sahagún-Ruiz

    Full Text Available Pasteurella pneumotropica is an opportunist Gram negative bacterium responsible for rodent pasteurellosis that affects upper respiratory, reproductive and digestive tracts of mammals. In animal care facilities the presence of P. pneumotropica causes severe to lethal infection in immunodeficient mice, being also a potential source for human contamination. Indeed, occupational exposure is one of the main causes of human infection by P. pneumotropica. The clinical presentation of the disease includes subcutaneous abscesses, respiratory tract colonization and systemic infections. Given the ability of P. pneumotropica to fully disseminate in the organism, it is quite relevant to study the role of the complement system to control the infection as well as the possible evasion mechanisms involved in bacterial survival. Here, we show for the first time that P. pneumotropica is able to survive the bactericidal activity of the human complement system. We observed that host regulatory complement C4BP and Factor H bind to the surface of P. pneumotropica, controlling the activation pathways regulating the formation and maintenance of C3-convertases. These results show that P. pneumotropica has evolved mechanisms to evade the human complement system that may increase the efficiency by which this pathogen is able to gain access to and colonize inner tissues where it may cause severe infections.

  11. Chromosome-based genetic complementation system for Xylella fastidiosa.

    Science.gov (United States)

    Matsumoto, Ayumi; Young, Glenn M; Igo, Michele M

    2009-03-01

    Xylella fastidiosa is a xylem-limited, gram-negative bacterium that causes Pierce's disease of grapevine. Here, we describe the construction of four vectors that facilitate the insertion of genes into a neutral site (NS1) in the X. fastidiosa chromosome. These vectors carry a colE1-like (pMB1) replicon and DNA sequences from NS1 flanking a multiple-cloning site and a resistance marker for one of the following antibiotics: chloramphenicol, erythromycin, gentamicin, or kanamycin. In X. fastidiosa, vectors with colE1-like (pMB1) replicons have been found to result primarily in the recovery of double recombinants rather than single recombinants. Thus, the ease of obtaining double recombinants and the stability of the resulting insertions at NS1 in the absence of selective pressure are the major advantages of this system. Based on in vitro and in planta characterizations, strains carrying insertions within NS1 are indistinguishable from wild-type X. fastidiosa in terms of growth rate, biofilm formation, and pathogenicity. To illustrate the usefulness of this system for complementation analysis, we constructed a strain carrying a mutation in the X. fastidiosa cpeB gene, which is predicted to encode a catalase/peroxidase, and showed that the sensitivity of this mutant to hydrogen peroxide could be overcome by the introduction of a wild-type copy of cpeB at NS1. Thus, this chromosome-based complementation system provides a valuable genetic tool for investigating the role of specific genes in X. fastidiosa cell physiology and virulence.

  12. Radioimmunoelectrophoresis, a sensitive method for detecting cleavage of the fifth component of human complement (C5)

    International Nuclear Information System (INIS)

    Perez, H.D.; Ong, R.; Banda, D.; Goldstein, I.M.

    1983-01-01

    A method has been developed for detecting cleavage of human C5 in serum and whole blood as a consequence of complement activation. Standard, single-dimension immunoelectrophoresis was performed using as antibody a radioiodinated IgG fraction prepared from a commercially available antiserum to human C5. Autoradiographs developed after radioimmunoelectrophoresis of either normal human serum or functionally pure human C5 revealed only one precipitin band. In contrast, when either zymosan-treated serum or trypsin-treated human C5 were examined with this technique, two additional precipitin bands were detected. One migrated more anodally than native C5 while the other remained at the origin (cathode). Radioimmunoelectrophoresis was significantly more sensitive as an indicator of complement activation in human serum than either measurements of total hemolytic complement or a standard assay for complement (C5)-derived chemotactic activity. (Auth.)

  13. Electronic components and systems

    CERN Document Server

    Dennis, W H

    2013-01-01

    Electronic Components and Systems focuses on the principles and processes in the field of electronics and the integrated circuit. Covered in the book are basic aspects and physical fundamentals; different types of materials involved in the field; and passive and active electronic components such as capacitors, inductors, diodes, and transistors. Also covered in the book are topics such as the fabrication of semiconductors and integrated circuits; analog circuitry; digital logic technology; and microprocessors. The monograph is recommended for beginning electrical engineers who would like to kn

  14. Specific, sensitive, precise, and rapid functional chromogenic assay of activated first complement component (C1) in plasma

    DEFF Research Database (Denmark)

    Munkvad, S; Jespersen, J; Sidelmann, Johannes Jakobsen

    1990-01-01

    We present a new functional assay for the first complement component (C1) in plasma, based on its activation by inhibition of the C1-esterase inhibitor (C1-inh) when monospecific antiserum to C1-inh is added to the plasma. After maximal activation, we can determine the concentration of activated ...

  15. The lectin pathway of complement activation is a critical component of the innate immune response to pneumococcal infection

    DEFF Research Database (Denmark)

    Ali, Youssif M; Lynch, Nicholas J; Haleem, Kashif S

    2012-01-01

    The complement system plays a key role in host defense against pneumococcal infection. Three different pathways, the classical, alternative and lectin pathways, mediate complement activation. While there is limited information available on the roles of the classical and the alternative activation...... to pneumococcal infection and fail to opsonize Streptococcus pneumoniae in the none-immune host. This defect in complement opsonisation severely compromises pathogen clearance in the lectin pathway deficient host. Using sera from mice and humans with defined complement deficiencies, we demonstrate that mouse...... of C4. This study corroborates the essential function of MASP-2 in the lectin pathway and highlights the importance of MBL-independent lectin pathway activation in the host defense against pneumococci....

  16. Genetic Variation in Complement Component 2 of the Classical Complement Pathway is Associated with Increased Mortality and Infection: A Study of 627 Trauma Patients

    Science.gov (United States)

    Morris, John A.; Francois, Cedric; Olson, Paul K.; Cotton, Bryan A.; Summar, Marshall; Jenkins, Judith M.; Norris, Patrick R.; Moore, Jason H.; Williams, Anna E.; McNew, Brent S.; Canter, Jeffrey A.

    2009-01-01

    Trauma is a disease of inflammation. Complement Component 2 (C2) is a protease involved in activation of complement through the classical pathway and has been implicated in a variety of chronic inflammatory diseases. We hypothesized that genetic variation in C2 (E318D) identifies a high-risk subgroup of trauma patients reflecting increased mortality and infection (Ventilator associated pneumonia: VAP). Consequently, genetic variation in C2 may stratify patient risk and illuminate underlying mechanisms for therapeutic intervention. Methods DNA samples from 702 trauma patients were genotyped for C2 E318D and linked with covariates (age: mean 42.8 years, gender: 74% male, ethnicity: 80% Caucasian, mechanism: 84% blunt, ISS: mean 25.0, admission lactate: mean 3.13 mEq/L) and outcomes: mortality 9.9% and VAP: 18.5%. VAP was defined by quantitative bronchoalveolar lavage (>104). Multivariate regression determined the relationship of genotype and covariates to risk of death and VAP. However, patients with ISS ≥ 45 were excluded from the multivariate analysis, as magnitude of injury overwhelms genetics and covariates in determining outcome. Results 52 patients (8.3%) had the high-risk heterozygous genotype, associated with a significant increase in mortality and VAP. Conclusion In 702 trauma patients, 8.3% had a high-risk genetic variation in C2 associated with increased mortality (OR=2.65) and infection (OR=2.00). This variation: 1) Identifies a previously unknown high risk group for infection and mortality; 2) Can be determined on admission; 3) May provide opportunity for early therapeutic intervention; and 4) Requires validation in a distinct cohort of patients. PMID:19430225

  17. The Complement System: A Prey of Trypanosoma cruzi

    Directory of Open Access Journals (Sweden)

    Kárita C. F. Lidani

    2017-04-01

    Full Text Available Trypanosoma cruzi is a protozoan parasite known to cause Chagas disease (CD, a neglected sickness that affects around 6–8 million people worldwide. Originally, CD was mainly found in Latin America but more recently, it has been spread to countries in North America, Asia, and Europe due the international migration from endemic areas. Thus, at present CD represents an important concern of global public health. Most of individuals that are infected by T. cruzi may remain in asymptomatic form all lifelong, but up to 40% of them will develop cardiomyopathy, digestive mega syndromes, or both. The interaction between the T. cruzi infective forms and host-related immune factors represents a key point for a better understanding of the physiopathology of CD. In this context, the complement, as one of the first line of host defense against infection was shown to play an important role in recognizing T. cruzi metacyclic trypomastigotes and in controlling parasite invasion. The complement consists of at least 35 or more plasma proteins and cell surface receptors/regulators, which can be activated by three pathways: classical (CP, lectin (LP, and alternative (AP. The CP and LP are mainly initiated by immune complexes or pathogen-associated molecular patterns (PAMPs, respectively, whereas AP is spontaneously activated by hydrolysis of C3. Once activated, several relevant complement functions are generated which include opsonization and phagocytosis of particles or microorganisms and cell lysis. An important step during T. cruzi infection is when intracellular trypomastigotes are release to bloodstream where they may be target by complement. Nevertheless, the parasite uses a sequence of events in order to escape from complement-mediated lysis. In fact, several T. cruzi molecules are known to interfere in the initiation of all three pathways and in the assembly of C3 convertase, a key step in the activation of complement. Moreover, T. cruzi promotes secretion

  18. Comprehensive approach to study complement C4 in systemic lupus erythematosus: Gene polymorphisms, protein levels and functional activity

    NARCIS (Netherlands)

    Tsang-A-Sjoe, M. W. P.; Bultink, I. E. M.; Korswagen, L. A.; van der Horst, A. [=Anneke; Rensink, I.; de Boer, M.; Hamann, D.; Voskuyl, A. E.; Wouters, D.

    2017-01-01

    Genetic variation of the genes encoding complement component C4 is strongly associated with systemic lupus erythematosus (SLE), a chronic multi-organ auto-immune disease. This study examined C4 and its isotypes on a genetic, protein, and functional level in 140 SLE patients and 104 healthy controls.

  19. Bothrops asper snake venom and its metalloproteinase BaP–1 activate the complement system. Role in leucocyte recruitment

    Directory of Open Access Journals (Sweden)

    Sandra H. P. Farsky

    2000-01-01

    Full Text Available The venom of the snake Bothrops asper, the most important poisonous snake in Central America, evokes an inflammatory response, the mechanisms of which are not well characterized. The objectives of this study were to investigate whether B. asper venom and its purified toxins – phospholipases and metalloproteinase – activate the complement system and the contribution of the effect on leucocyte recruitment. In vitro chemotaxis assays were performed using Boyden's chamber model to investigate the ability of serum incubated with venom and its purified toxins to induce neutrophil migration. The complement consumption by the venom was evaluated using an in vitro haemolytic assay. The importance of complement activation by the venom on neutrophil migration was investigated in vivo by injecting the venom into the peritoneal cavity of C5-deficient mice. Data obtained demonstrated that serum incubated with crude venom and its purified metalloproteinase BaP–1 are able to induce rat neutrophil chemotaxis, probably mediated by agent(s derived from the complement system. This hypothesis was corroborated by the capacity of the venom to activate this system in vitro. The involvement of C5a in neutrophil chemotaxis induced by venom-activated serum was demonstrated by abolishing migration when neutrophils were pre-incubated with antirat C5a receptor antibody. The relevance of the complement system in in vivo leucocyte mobilization was further demonstrated by the drastic decrease of this response in C5-deficient mice. Pre-incubation of serum with the soluble human recombinant complement receptor type 1 (sCR 1 did not prevent the response induced by the venom, but abolished the migration evoked by metalloproteinase-activated serum. These data show the role of the complement system in bothropic envenomation and the participation of metalloproteinase in the effect. Also, they suggest that the venom may contain other component(s which can cause direct activation

  20. Is complement good, bad, or both? New functions of the complement factors associated with inflammation mechanisms in the central nervous system.

    Science.gov (United States)

    Tahtouh, Muriel; Croq, Françoise; Lefebvre, Christophe; Pestel, Joël

    2009-09-01

    The complement system is well known as an enzyme cascade that helps to defend against infections. Indeed, this ancestral system bridges innate and adaptive immunity. Its implication in diseases of the central nervous system (CNS), has led to an increased number of studies. Complement activation in the CNS has been generally considered to contribute to tissue damage. However, recent studies suggest that complement may be neuroprotective, and can participate in maintenance and repair of the adult brain. Here, we will review this dual role of complement proteins and some of their functional interactions with part of the chemokine and cytokine network associated with the protection of CNS integrity.

  1. Staphylococcal Proteases Aid in Evasion of the Human Complement System

    DEFF Research Database (Denmark)

    Jusko, Monika; Potempa, Jan; Kantyka, Tomasz

    2014-01-01

    Staphylococcus aureus is an opportunistic pathogen that presents severe health care concerns due to the prevalence of multiple antibiotic-resistant strains. New treatment strategies are urgently needed, which requires an understanding of disease causation mechanisms. Complement is one of the first...

  2. The structure of C2b, a fragment of complement component C2 produced during C3 convertase formation

    Energy Technology Data Exchange (ETDEWEB)

    Krishnan, Vengadesan [Center for Biophysical Sciences and Engineering, School of Optometry, University of Alabama at Birmingham, Birmingham, AL 35294 (United States); Xu, Yuanyuan [Division of Clinical Immunology and Rheumatology, University of Alabama at Birmingham, Birmingham, AL 35294 (United States); Macon, Kevin [Center for Biophysical Sciences and Engineering, School of Optometry, University of Alabama at Birmingham, Birmingham, AL 35294 (United States); Volanakis, John E. [Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294 (United States); Narayana, Sthanam V. L., E-mail: narayana@uab.edu [Center for Biophysical Sciences and Engineering, School of Optometry, University of Alabama at Birmingham, Birmingham, AL 35294 (United States)

    2009-03-01

    The crystal structure of C2b has been determined at 1.8 Å resolution, which reveals the arrangement of its three complement control protein (CCP) modules. A model for complement component C2 is presented and its conformational changes during the C3-convertase formation are also discussed. The second component of complement (C2) is a multi-domain serine protease that provides catalytic activity for the C3 and C5 convertases of the classical and lectin pathways of human complement. The formation of these convertases requires the Mg{sup 2+}-dependent binding of C2 to C4b and the subsequent cleavage of C2 by C1s or MASP2, respectively. The crystal structure of full-length C2 is not yet available, although the structure of its C-terminal catalytic segment C2a has been determined. The crystal structure of the N-terminal segment C2b of C2 determined to 1.8 Å resolution presented here reveals the arrangement of its three CCP domains. The domains are arranged differently compared with most other CCP-domain assemblies, but their arrangement is similar to that found in the Ba part of the full-length factor B structure. The crystal structures of C2a, C2b and full-length factor B are used to generate a model for C2 and a discussion of the domain association and possible interactions with C4b during formation of the C4b–C2 complex is presented. The results of this study also suggest that upon cleavage by C1s, C2a domains undergo conformational rotation while bound to C4b and the released C2b domains may remain folded together similar to as observed in the intact protein.

  3. The structure of C2b, a fragment of complement component C2 produced during C3 convertase formation

    International Nuclear Information System (INIS)

    Krishnan, Vengadesan; Xu, Yuanyuan; Macon, Kevin; Volanakis, John E.; Narayana, Sthanam V. L.

    2009-01-01

    The crystal structure of C2b has been determined at 1.8 Å resolution, which reveals the arrangement of its three complement control protein (CCP) modules. A model for complement component C2 is presented and its conformational changes during the C3-convertase formation are also discussed. The second component of complement (C2) is a multi-domain serine protease that provides catalytic activity for the C3 and C5 convertases of the classical and lectin pathways of human complement. The formation of these convertases requires the Mg 2+ -dependent binding of C2 to C4b and the subsequent cleavage of C2 by C1s or MASP2, respectively. The crystal structure of full-length C2 is not yet available, although the structure of its C-terminal catalytic segment C2a has been determined. The crystal structure of the N-terminal segment C2b of C2 determined to 1.8 Å resolution presented here reveals the arrangement of its three CCP domains. The domains are arranged differently compared with most other CCP-domain assemblies, but their arrangement is similar to that found in the Ba part of the full-length factor B structure. The crystal structures of C2a, C2b and full-length factor B are used to generate a model for C2 and a discussion of the domain association and possible interactions with C4b during formation of the C4b–C2 complex is presented. The results of this study also suggest that upon cleavage by C1s, C2a domains undergo conformational rotation while bound to C4b and the released C2b domains may remain folded together similar to as observed in the intact protein

  4. R102G polymorphism of the complement component 3 gene in Malaysian subjects with neovascular age-related macular degeneration

    Directory of Open Access Journals (Sweden)

    Nur Afiqah Mohamad

    2018-04-01

    Full Text Available Background: Genetic and environmental factors are known to be risk factors in development of neovascular age-related macular degeneration (nAMD. Genetic factors such as polymorphisms in the complement component pathway genes might play a role in pathogenesis of nAMD and has been studied in various populations excluding Malaysia. Aim of the study: To determine the association of the R102G polymorphism of the complement component (C3 gene in nAMD subjects. Patients and methods: A total of 301 Malaysian subjects (149 case and 152 controls were recruited and genotyped for the R102G (rs2230199 variant of the C3 gene. Genotyping was conducted using the PCR-RFLP method and association analysis was conducted using appropriate statistical tests. Results: From our findings, no significant association was observed in the allele distribution of C3 R102G between nAMD and controls (OR = 1.42, 95% CI = 0.77–2.62, P = 0.268. A further analysis that compared three genetic models (dominant, recessive and co-dominant also recorded no significant difference (P > 0.05. These findings could be due to the low frequency of the GG variant in the case (4.7% and control (1.3% groups, compared to the normal variant CC, which is present in 91.3% of case and 92.8% of control alleles. Conclusion: The present study showed no evidence of association between C3 R102G polymorphism and nAMD in Malaysian subjects. Keywords: Age-related macular degeneration, Complement component 3, C3 gene, R102G gene polymorphism

  5. Storage of the complement components C4, C3, and C 3-activator in the human liver as PAS-negative globular hyaline bodies.

    Science.gov (United States)

    Storch, W; Riedel, H; Trautmann, B; Justus, J; Hiemann, D

    1982-01-01

    Liver biopsies of a 58-year-old clinically healthy patient with a hepatomegaly and intracisternal PAS-negative globular hyaline bodies were immunofluorescent-optically examined for the content of the complement components C 1 q, C 4, C 9, C 1-inactivator, C 3-activator. Further examinations were performed for fibrinogen, IgG, IgA, IgM, IgD, IgE, L-chain (type chi and lambda), alpha 1-antitrypsin, alpha 1-fetoprotein, alpha 1- and alpha 2-glycoprotein, cholinesterase, ceruloplasmin, myoglobin, hemopexin, HBsAg and HBsAg. Th inclusion bodies reacted with antisera against the complement components C 4, C 3 and C 3-activator, as also identified by double immunofluorescence. Probably this is a disturbance of the protein metabolism of the liver cell with abnormal complement storage in the presence of normal total complement and normal complement components in the serum.

  6. Immune complex modulation by plasma proteins. With special reference to the complement system and autoimmune diseases

    DEFF Research Database (Denmark)

    Baatrup, G

    1989-01-01

    The complement (C) system consists of two activation pathways, the classical and the alternative, which may both be activated by immune complexes (IC). C activation products become attached to the IC during activation leading to profound changes in the properties of the complexes. The common...... inflammation. 5) Tissue damage by activation and/or lysis of bystanding cells. 6) Modulation of B-cell proliferation and differentiation. Activation of the C system by IC is an essential normal component in the clearance of invading foreign material. However, in conditions with a persistent high concentration...... preformed, fluid phase IC (CMS assay). The CMS was found to be dependent upon the alternative pathway of C and facilitated by the classical. Further studies concerning the influence of C deficiencies or depletion of C factors, the concentration of divalent metallions, the temperature and the ionic strength...

  7. Molecular and expression analysis of complement component C5 in the nurse shark (Ginglymostoma cirratum) and its predicted functional role.

    Science.gov (United States)

    Graham, Matthew; Shin, Dong-Ho; Smith, Sylvia L

    2009-07-01

    We present the complete cDNA sequence of shark (Ginglymostoma cirratum) pro-C5 and its molecular characterization with a descriptive analysis of the structural elements necessary for its potential functional role as a potent mediator of inflammation (fragment C5a) and initiator molecule (fragment C5b) for the assembly of the membrane attack complex (MAC) upon activation by C5 convertase. In mammals the three complement activation cascades, the classical, alternative and lectin pathways, converge at the activation of C3, a pivotal complement protein. It is, however, the subsequent activation of the next complement component, C5, which is the focal point at which the initiation of the terminal lytic pathway takes place and involves the stepwise assembly of the MAC. The effector cytolytic function of complement occurs with the insertion of MAC into target membranes causing dough-nut like holes and cell leakage. The lytic activity of shark complement results in structurally similar holes in target membranes suggesting the assembly of a shark MAC that likely involves a functional analogue of C5. The composition of shark MAC remains unresolved and to date conclusive evidence has been lacking for shark C5. The gene has not been cloned nor has the serum protein been characterized for any elasmobranch species. This report is the first to confirm the presence of C5 homologue in the shark. GcC5 is remarkably similar to human C5 in overall structure and domain arrangement. The GcC5 cDNA measured 5160-bp with 5' and 3' UTRs of 35 bp and 79 bp, respectively. Structural analysis of the derived protein sequence predicts a molecule that is a two-chain structure which lacks a thiolester bond and contains a C5 convertase cleavage site indicating that activation will generate two peptides, akin to C5b and C5a. The putative GcC5 molecule also contains the C-terminal C345C/Netrin module that characterizes C3, C4 and C5. Multiple alignment of deduced amino acid sequences shows that GcC5

  8. Soluble and immobilized graphene oxide activates complement system differently dependent on surface oxidation state

    DEFF Research Database (Denmark)

    Wibroe, Peter Popp; Petersen, Søren Vermehren; Bovet, Nicolas Emile

    2016-01-01

    on two related elements of innate immunity: the complement system and interleukin-6 (IL-6) release in human blood. In solution, there was a decrease in GO-mediated complement activation with decreasing surface oxygen content (and altered oxygen functionality), whereas with immobilized GO complement...... response were reversed and increased with decreasing oxygen content. GO solutions, at concentrations below complement activating threshold, did not induce IL-6 release from human blood leukocytes, and further dampened lipopolysaccharide-induced IL-6 release in the whole blood. The latter effect became more...... profound with GO's having higher oxygen content. This protective role of GO solutions, however, disappeared at higher concentrations above complement-activating threshold. We discuss these results in relation to GO surface structure and properties, and implications for local administration and development...

  9. Complement receptor expression and activation of the complement cascade on B lymphocytes from patients with systemic lupus erythematosus (SLE)

    DEFF Research Database (Denmark)

    Marquart, H V; Svendsen, A; Rasmussen, J M

    1995-01-01

    It has previously been reported that the expression of the complement receptors, CR1 on erythrocytes and blood leucocytes and CR2 on B cells, is reduced in patients with SLE, and that the reduced expression of CR1 on erythrocytes is related to disease activity. We have earlier demonstrated...... that normal B cells are capable of activating the alternative pathway (AP) of complement in a CR2-dependent fashion. In this study we have investigated whether disturbances in this activity may be related to the altered phenotype of SLE B cells. Flow cytometry was used to measure expression of complement...

  10. Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration

    Directory of Open Access Journals (Sweden)

    Klein Michael L

    2011-07-01

    Full Text Available Abstract Background Age related macular degeneration (AMD is the leading cause of irreversible blindness in elderly populations worldwide. Inflammation, among many factors, has been suggested to play an important role in AMD pathogenesis. Recent studies have demonstrated a strong genetic association between AMD and complement factor H (CFH, the down-regulatory factor of complement activation. Elevated levels of complement activating molecules including complement component 5a (C5a have been found in the serum of AMD patients. Our aim is to study whether C5a can impact human T cells and its implication in AMD. Methods Human peripheral blood mononuclear cells (PBMCs were isolated from the blood of exudative form of AMD patients using a Ficoll gradient centrifugation protocol. Intracellular staining and enzyme-linked immunosorbent assays were used to measure protein expression. Apoptotic cells were detected by staining of cells with the annexin-V and TUNEL technology and analyzed by a FACS Caliber flow cytometer. SNP genotyping was analyzed by TaqMan genotyping assay using the Real-time PCR system 7500. Results We show that C5a promotes interleukin (IL-22 and IL-17 expression by human CD4+ T cells. This effect is dependent on B7, IL-1β and IL-6 expression from monocytes. We have also found that C5a could protect human CD4+ cells from undergoing apoptosis. Importantly, consistent with a role of C5a in promoting IL-22 and IL-17 expression, significant elevation in IL-22 and IL-17 levels was found in AMD patients as compared to non-AMD controls. Conclusions Our results support the notion that C5a may be one of the factors contributing to the elevated serum IL-22 and IL-17 levels in AMD patients. The possible involvement of IL-22 and IL-17 in the inflammation that contributes to AMD may herald a new approach to treat AMD.

  11. Activity and activation of the complement system in patients being operated on for cancer of the colon

    DEFF Research Database (Denmark)

    Baatrup, G; Qvist, N; Junker, A

    1994-01-01

    OBJECTIVE: To find out if there was any local activation of complement in the vicinity of a colonic cancer, and any fluctuation in the function of the complement system during operation. DESIGN: Prospective study. SETTING: One university and two district hospitals in Denmark. SUBJECTS: 29 selected...... patients undergoing emergency and elective operations for colonic cancer. INTERVENTIONS: Measurements of systemic and local complement fixation capacity and complement activation in samples of serum or plasma taken before, during, and after operation. MAIN OUTCOME MEASURES: Changes in complement fixation...... capacity and complement activation during operation. RESULTS: Haemodilution during operation caused a significant reduction in the complement fixation capacity of serum and in the activation of the complement system as measured by generation of C3c. We were unable to confirm the presence of complement...

  12. SNR coolant system components

    International Nuclear Information System (INIS)

    De Haas Van Dorsser, A.H.; Mausbeck, H.

    1976-01-01

    The DEBENELUX prototype fast reactor power plant SNR 300 at Kalkar has a loop-type heat transfer system similar to that of the prototype LMFBR plants in the USA and Japan. There exist three 257 MW/sub th/ primary sodium loops, each with a hot leg centrifugal pump and three 85.6 MW/sub th/ intermediate heat exchangers in parallel. From there the heat is transferred to the steam generators via three secondary sodium loops with one cold leg sodium circulating pump in each. At a nominal reactor outlet temperature of 819 0 K and a turbine inlet power of 771 MW/sub th/ super heated steam of 166 bar and 733 0 K is produced, giving rise to a plant rating of 327 MW/sub e/ gross. The primary and secondary loops are described in detail

  13. Isolation and initial characterisation of complement components C3 and C4 of the nurse shark and the channel catfish.

    Science.gov (United States)

    Dodds, A W; Smith, S L; Levine, R P; Willis, A C

    1998-01-01

    Complement components C3 and C4 have been isolated from the serum of the nurse shark (Ginglymostoma cirratum) and of the channel catfish (Ictalurus punctatus). As in the higher vertebrates, the fish C4 proteins have three-chain structures while the C3 proteins have two-chain structures. All four proteins have intra-chain thioesters located within their highest molecular mass polypeptides. N-terminal sequence analysis of the polypeptides has confirmed the identity of the proteins. In all cases except the catfish C3 alpha-chain, which appears to have a blocked N-terminus, sequence similarities are apparent in comparisons with the chains of C3 and C4 from higher vertebrates. We have confirmed that the activity/protein previously designated C2n is the nurse shark analogue of mammalian C4. This is the first report of structural evidence for C4 in both the bony and cartilaginous fish.

  14. The influence of gamma radiation upon the biological activity of the third serum complement component (C3)

    International Nuclear Information System (INIS)

    Steuhl, K.P.; Dierich, M.P.; Mainz Univ.

    1981-01-01

    For investigation of interaction between C3 and C3-binding cells the third complement component is to be labelled with radiotracer. After labelling C3 with high specific activity (0,2 μCi 125 l/μg C3) binding of C3 to Raji-cells was increased up to the twentyfold nine days after labelling. This effect was not to be reproduced with external gamma radiation using doses of 10, 200 and 1000 rad. The rosette inhibition test could demonstrate that with radiation doses of 200 and 1000 rad the radiated C3 lost its ability of specific binding to C3 receptors in Raji-cells. This functional alteration corresponded to amino acid analysis with relative increase of asparagine, glutamic acid and proline and relative decrease of cystine and phenylalanine in the C3 molecule. (orig.) [de

  15. Complement component C1r mediated cleavage of the heavy chain of the major histocompatibility class I antigens

    DEFF Research Database (Denmark)

    Eriksson, H; Nissen, Mogens Holst

    1992-01-01

    Apart from cleaving C1s, we demonstrate for the first time that: 1) at concentrations found in serum, the activated forms of the complement components C1r in addition to C1s can cleave the heavy chain of MHC class I antigens, 2) the cleavage by C1r and C1s is seemingly dependent upon a native con......-chain of MHC class I was shown to take place between the alpha 2- and alpha 3- domains as estimated by the Con A-Sepharose precipitation pattern on SDS-PAGE. The alpha 1/alpha 2 fragment was still shown to interact with beta 2-microglobulin as shown by immunoprecipitation....

  16. Component reliability for electronic systems

    CERN Document Server

    Bajenescu, Titu-Marius I

    2010-01-01

    The main reason for the premature breakdown of today's electronic products (computers, cars, tools, appliances, etc.) is the failure of the components used to build these products. Today professionals are looking for effective ways to minimize the degradation of electronic components to help ensure longer-lasting, more technically sound products and systems. This practical book offers engineers specific guidance on how to design more reliable components and build more reliable electronic systems. Professionals learn how to optimize a virtual component prototype, accurately monitor product reliability during the entire production process, and add the burn-in and selection procedures that are the most appropriate for the intended applications. Moreover, the book helps system designers ensure that all components are correctly applied, margins are adequate, wear-out failure modes are prevented during the expected duration of life, and system interfaces cannot lead to failure.

  17. Component Reification in Systems Modelling

    DEFF Research Database (Denmark)

    Bendisposto, Jens; Hallerstede, Stefan

    When modelling concurrent or distributed systems in Event-B, we often obtain models where the structure of the connected components is specified by constants. Their behaviour is specified by the non-deterministic choice of event parameters for events that operate on shared variables. From a certain......? These components may still refer to shared variables. Events of these components should not refer to the constants specifying the structure. The non-deterministic choice between these components should not be via parameters. We say the components are reified. We need to address how the reified components get...... reflected into the original model. This reflection should indicate the constraints on how to connect the components....

  18. F(ab'2 antibody fragments against Trypanosoma cruzi calreticulin inhibit its interaction with the first component of human complement

    Directory of Open Access Journals (Sweden)

    LORENA AGUILAR

    2005-01-01

    Full Text Available Trypanosoma cruzi calreticulin (TcCRT, described in our laboratory, retains several important functional features from its vertebrate homologues. We have shown that recombinant TcCRT inhibits the human complement system when it binds to the collagenous portion of C1q. The generation of classical pathway convertases and membrane attack complexes is thus strongly inhibited. In most T. cruzi-infected individuals, TcCRT is immunogenic and mediates the generation of specific antibodies. By reverting the C1q / TcCRT interaction, a parasite immune evasion strategy, these antibodies contribute to the host / parasite equilibrium. In an in vitro correlate of this situation, we show that the C1q / TcCRT interaction is inhibited by F(ab'2 polyclonal anti-TcCRT IgG fragments. It is therefore feasible that in infected humans anti-TcCRT antibodies participate in reverting an important parasite strategy aimed at inhibiting the classical complement pathway. Thus, membrane-bound TcCRT interacts with the collagenous portion C1q, and this C1q is recognized by the CD91-bound host cell CRT, thus facilitating parasite internalization. Based on our in vitro results, it could be proposed that the in vivo interaction between TcCRT and vertebrate C1q could be inhibited by F(ab'2 fragments anti-rTcCRT or against its S functional domain, thus interfering with the internalization process

  19. Human alpha2-macroglobulin is composed of multiple domains, as predicted by homology with complement component C3.

    Science.gov (United States)

    Doan, Ninh; Gettins, Peter G W

    2007-10-01

    Human alpha2M (alpha2-macroglobulin) and the complement components C3 and C4 are thiol ester-containing proteins that evolved from the same ancestral gene. The recent structure determination of human C3 has allowed a detailed prediction of the location of domains within human alpha2M to be made. We describe here the expression and characterization of three alpha(2)M domains predicted to be involved in the stabilization of the thiol ester in native alpha2M and in its activation upon bait region proteolysis. The three newly expressed domains are MG2 (macroglobulin domain 2), TED (thiol ester-containing domain) and CUB (complement protein subcomponents C1r/C1s, urchin embryonic growth factor and bone morphogenetic protein 1) domain. Together with the previously characterized RBD (receptor-binding domain), they represent approx. 42% of the alpha2M polypeptide. Their expression as folded domains strongly supports the predicted domain organization of alpha2M. An X-ray crystal structure of MG2 shows it to have a fibronectin type-3 fold analogous to MG1-MG8 of C3. TED is, as predicted, an alpha-helical domain. CUB is a spliced domain composed of two stretches of polypeptide that flank TED in the primary structure. In intact C3 TED interacts with RBD, where it is in direct contact with the thiol ester, and with MG2 and CUB on opposite, flanking sides. In contrast, these alpha2M domains, as isolated species, show negligible interaction with one another, suggesting that the native conformation of alpha2M, and the consequent thiol ester-stabilizing domain-domain interactions, result from additional restraints imposed by the physical linkage of these domains or by additional domains in the protein.

  20. Human α2-macroglobulin is composed of multiple domains, as predicted by homology with complement component C3

    Science.gov (United States)

    Doan, Ninh; Gettins, Peter G. W.

    2007-01-01

    Human α2M (α2-macroglobulin) and the complement components C3 and C4 are thiol ester-containing proteins that evolved from the same ancestral gene. The recent structure determination of human C3 has allowed a detailed prediction of the location of domains within human α2M to be made. We describe here the expression and characterization of three α2M domains predicted to be involved in the stabilization of the thiol ester in native α2M and in its activation upon bait region proteolysis. The three newly expressed domains are MG2 (macroglobulin domain 2), TED (thiol ester-containing domain) and CUB (complement protein subcomponents C1r/C1s, urchin embryonic growth factor and bone morphogenetic protein 1) domain. Together with the previously characterized RBD (receptor-binding domain), they represent approx. 42% of the α2M polypeptide. Their expression as folded domains strongly supports the predicted domain organization of α2M. An X-ray crystal structure of MG2 shows it to have a fibronectin type-3 fold analogous to MG1–MG8 of C3. TED is, as predicted, an α-helical domain. CUB is a spliced domain composed of two stretches of polypeptide that flank TED in the primary structure. In intact C3 TED interacts with RBD, where it is in direct contact with the thiol ester, and with MG2 and CUB on opposite, flanking sides. In contrast, these α2M domains, as isolated species, show negligible interaction with one another, suggesting that the native conformation of α2M, and the consequent thiol ester-stabilizing domain–domain interactions, result from additional restraints imposed by the physical linkage of these domains or by additional domains in the protein. PMID:17608619

  1. Using cloud technologies to complement environmental information systems

    International Nuclear Information System (INIS)

    Schlachter, Thorsten; Duepmeier, Clemens; Weidemann, Rainer

    2013-01-01

    Cloud services can help to close the gap between available and published data by providing infrastructure, storage, services, or even whole applications. Within this paper we present some fundamental ideas on the use of cloud services for the construction of powerful services in order to toughen up environmental information systems for the needs of state of the art web, portal, and mobile technologies. We include uses cases for the provision of environmental information as well as for the collection of user generated data. (orig.)

  2. A versatile selection system for folding competent proteins using genetic complementation in a eukaryotic host

    DEFF Research Database (Denmark)

    Lyngsø, C.; Kjaerulff, S.; Muller, S.

    2010-01-01

    in vivo selection system for folded proteins. It is based on genetic complementation of the Schizosaccharomyces pombe growth marker gene invertase fused C-terminally to a protein library. The fusion proteins are directed to the secretion system, utilizing the ability of the eukaryotic protein quality...

  3. Ficolins and the lectin pathway of complement in patients with systemic lupus erythematosus

    DEFF Research Database (Denmark)

    Hein, Estrid; Nielsen, Louise Aas; Nielsen, Christoffer T

    2015-01-01

    The complement system plays a pathophysiological role in systemic lupus erythematosus (SLE). This study aims to investigate whether an association exists between the ficolins that are part of the lectin complement pathway and SLE. EDTA plasma samples from 68 Danish SLE patients and 29 healthy...... Lupus International Collaborating Clinics (SLICC)/American College of Rheumatology (ACR) Damage Index] (SDI) (Rho=0.27, P=0.026). The Ficolin-1 concentration was also associated with the occurrence of arterial (P=0.0053) but not venous thrombosis (P=0.42). Finally, deposition of C4, C3 and TCC...

  4. The complement system: a gateway to gene-environment interactions in schizophrenia pathogenesis.

    Science.gov (United States)

    Nimgaonkar, V L; Prasad, K M; Chowdari, K V; Severance, E G; Yolken, R H

    2017-11-01

    The pathogenesis of schizophrenia is considered to be multi-factorial, with likely gene-environment interactions (GEI). Genetic and environmental risk factors are being identified with increasing frequency, yet their very number vastly increases the scope of possible GEI, making it difficult to identify them with certainty. Accumulating evidence suggests a dysregulated complement pathway among the pathogenic processes of schizophrenia. The complement pathway mediates innate and acquired immunity, and its activation drives the removal of damaged cells, autoantigens and environmentally derived antigens. Abnormalities in complement functions occur in many infectious and autoimmune disorders that have been linked to schizophrenia. Many older reports indicate altered serum complement activity in schizophrenia, though the data are inconclusive. Compellingly, recent genome-wide association studies suggest repeat polymorphisms incorporating the complement 4A (C4A) and 4B (C4B) genes as risk factors for schizophrenia. The C4A/C4B genetic associations have re-ignited interest not only in inflammation-related models for schizophrenia pathogenesis, but also in neurodevelopmental theories, because rodent models indicate a role for complement proteins in synaptic pruning and neurodevelopment. Thus, the complement system could be used as one of the 'staging posts' for a variety of focused studies of schizophrenia pathogenesis. They include GEI studies of the C4A/C4B repeat polymorphisms in relation to inflammation-related or infectious processes, animal model studies and tests of hypotheses linked to autoimmune diseases that can co-segregate with schizophrenia. If they can be replicated, such studies would vastly improve our understanding of pathogenic processes in schizophrenia through GEI analyses and open new avenues for therapy.

  5. Complement Activation in Inflammatory Skin Diseases

    Directory of Open Access Journals (Sweden)

    Jenny Giang

    2018-04-01

    Full Text Available The complement system is a fundamental part of the innate immune system, playing a crucial role in host defense against various pathogens, such as bacteria, viruses, and fungi. Activation of complement results in production of several molecules mediating chemotaxis, opsonization, and mast cell degranulation, which can contribute to the elimination of pathogenic organisms and inflammation. Furthermore, the complement system also has regulating properties in inflammatory and immune responses. Complement activity in diseases is rather complex and may involve both aberrant expression of complement and genetic deficiencies of complement components or regulators. The skin represents an active immune organ with complex interactions between cellular components and various mediators. Complement involvement has been associated with several skin diseases, such as psoriasis, lupus erythematosus, cutaneous vasculitis, urticaria, and bullous dermatoses. Several triggers including auto-antibodies and micro-organisms can activate complement, while on the other hand complement deficiencies can contribute to impaired immune complex clearance, leading to disease. This review provides an overview of the role of complement in inflammatory skin diseases and discusses complement factors as potential new targets for therapeutic intervention.

  6. Complement system proteins which interact with C3b or C4b A superfamily of structurally related proteins

    DEFF Research Database (Denmark)

    Reid, K B M; Bentley, D R; Campbell, R D

    1986-01-01

    Recent cDNA sequencing data has allowed the prediction of the entire amino acid sequences of complement components factor B and C2, the complement control proteins factor H and C4b-binding protein and a partial sequence for the Cab/C4b receptor CR1. These proteins all contain internal repeating u...

  7. The membrane attack complex of the complement system is essential for rapid wallerian degeneration

    NARCIS (Netherlands)

    Ramaglia, Valeria; King, Rosalind Helen Mary; Nourallah, Michelle; Wolterman, Ruud; de Jonge, Rosalein; Ramkema, Marja; Vigar, Miriam Ann; van der Wetering, Sandra; Morgan, Brian Paul; Troost, Dirk; Baas, Frank

    2007-01-01

    The complement (C) system plays an important role in myelin breakdown during Wallerian degeneration (WD). The pathway and mechanism involved are, however, not clear. In a crush injury model of the sciatic nerve, we show that C6, necessary for the assembly of the membrane attack complex (MAC), is

  8. Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches

    Science.gov (United States)

    Franc, Vojtech; Zhu, Jing; Heck, Albert J. R.

    2018-03-01

    The human complement hetero-trimeric C8αβγ (C8) protein assembly ( 150 kDa) is an important component of the membrane attack complex (MAC). C8 initiates membrane penetration and coordinates MAC pore formation. Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics. The intact C8 proteoform profile revealed at least 20 co-occurring MS signals. Additionally, we employed ion exchange chromatography to separate purified C8 into four distinct fractions. Their native MS analysis revealed even more detailed structural micro-heterogeneity on C8. Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation. Combining all this data provides a detailed specification of co-occurring C8 proteoforms, including experimental evidence on N-glycosylation, C-mannosylation, and O-glycosylation. In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8. Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β. Lastly, our data contain the first experimental evidence of O-linked glycans located on C8γ. Albeit low abundant, these O-glycans are the first PTMs ever detected on this subunit. By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation. [Figure not available: see fulltext.

  9. Human genes for complement components C1r and C1s in a close tail-to-tail arrangement

    International Nuclear Information System (INIS)

    Kusumoto, H.; Hirosawa, S.; Salier, J.P.; Hagen, F.S.; Kurachi, K.

    1988-01-01

    Complementary DNA clones for human C1s were isolated from cDNA libraries that were prepared with poly(A) + RNAs of human liver and HepG2 cells. A clone with the largest cDNA insert of 2,664 base pairs (bp) was analyzed for its complete nucleotide sequence. It contained 202 bp of a 5' untranslated region, 45 bp of coding for a signal peptide (15 amino acid residues), 2,019 bp for complement component C1s zymogen (673 amino acid residues), 378 bp for a 3' untranslated region, a stop codon, and 17 bp of a poly(A) tail. The amino acid sequence of C1s was 40.5% identical to that of C1r, with excellent matches of tentative disulfide bond locations conserving the overall domain structure of C1r. DNA blotting and sequencing analyses of genomic DNA and of an isolated genomic DNA clone clearly showed that the human genes for C1r and C1s are closely located in a tail-to-tail arrangement at a distance of about 9.5 kilobases. Furthermore, RNA blot analyses showed that both C1r and C1s genes are primarily expressed in liver, whereas most other tissues expressed both C1r and C1s genes at much lower levels (less than 10% of that in liver). Multiple molecular sizes of specific mRNAs were observed in the RNA blot analyses for both C1r and C1s, indicating that alternative RNA processing(s), likely an alternative polyadenylylation, might take place for both genes

  10. 17Beta-estradiol affects the response of complement components and survival of rainbow trout (Oncorhynchus mykiss) challenged by bacterial infection.

    Science.gov (United States)

    Wenger, Michael; Sattler, Ursula; Goldschmidt-Clermont, Elinor; Segner, Helmut

    2011-07-01

    Research on the endocrine role of estrogens has focused on the reproductive system, while other potential target systems have been less studied. Here, we investigated the possible immunomodulating role of 17β-estradiol (E2) using rainbow trout (Oncorhynchus mykiss) as a model. The aims of the study were to examine a) whether estrogens can modulate immune gene transcription levels, and b) whether this has functional implications for the resistance of trout towards pathogens. Trout were reared from fertilization until 6 months of age under (1) control conditions, (2) short-term E2-treatment (6-month-old juveniles were fed a diet containing 20 mg E2/kg for 2 weeks), or c) long-term E2-treatment (twice a 2-h-bath-exposure of trout embryos to 400 μg 17β-estradiol (E2)/L, followed by rearing on the E2-spiked diet from start-feeding until 6 months of age). Analysis of plasma estrogen levels indicated that the internal estrogen concentrations of E2-exposed fish were within the physiological range and analysis of hepatic vitellogenin mRNA levels indicated that the E2 administration was effective in activating the endogenous estrogen receptor pathway. However, expression levels of the hepatic complement components C3-1, C3-3, and Factor H were not affected by E2-treatment. In a next step, 6-month-old juveniles were challenged with pathogenic bacteria (Yersinia ruckeri). In control fish, this bacterial infection resulted in significant up-regulation of the mRNA levels of hepatic complement genes (C3-1, C3-3, Factor B, Factor H), while E2-treated fish showed no or significantly lower up-regulation of the complement gene transcription levels. Apparently, the E2-treated trout had a lower capacity to activate their immune system to defend against the bacterial infection. This interpretation is corroborated by the finding that survival of E2-treated fish under bacterial challenge was significantly lower than in the control group. In conclusion, the results from this study

  11. Calcineurin inhibitor-induced complement system activation via ERK1/2 signalling is inhibited by SOCS-3 in human renal tubule cells.

    Science.gov (United States)

    Loeschenberger, Beatrix; Niess, Lea; Würzner, Reinhard; Schwelberger, Hubert; Eder, Iris E; Puhr, Martin; Guenther, Julia; Troppmair, Jakob; Rudnicki, Michael; Neuwirt, Hannes

    2018-02-01

    One factor that significantly contributes to renal allograft loss is chronic calcineurin inhibitor (CNI) nephrotoxicity (CIN). Among other factors, the complement (C-) system has been proposed to be involved CIN development. Hence, we investigated the impact of CNIs on intracellular signalling and the effects on the C-system in human renal tubule cells. In a qPCR array, CNI treatment upregulated C-factors and downregulated SOCS-3 and the complement inhibitors CD46 and CD55. Additionally, ERK1/-2 was required for these regulations. Following knock-down and overexpression of SOCS-3, we found that SOCS-3 inhibits ERK1/-2 signalling. Finally, we assessed terminal complement complex formation, cell viability and apoptosis. Terminal complement complex formation was induced by CNIs. Cell viability was significantly decreased, whereas apoptosis was increased. Both effects were reversed under complement component-depleted conditions. In vivo, increased ERK1/-2 phosphorylation and SOCS-3 downregulation were observed at the time of transplantation in renal allograft patients who developed a progressive decline of renal function in the follow-up compared to stable patients. The progressive cohort also had lower total C3 levels, suggesting higher complement activity at baseline. In conclusion, our data suggest that SOCS-3 inhibits CNI-induced ERK1/-2 signalling, thereby blunting the negative control of C-system activation. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. Age-related macular degeneration and modification of systemic complement factor H production through liver transplantation.

    Science.gov (United States)

    Khandhadia, Samir; Hakobyan, Svetlana; Heng, Ling Z; Gibson, Jane; Adams, David H; Alexander, Graeme J; Gibson, Jonathan M; Martin, Keith R; Menon, Geeta; Nash, Kathryn; Sivaprasad, Sobha; Ennis, Sarah; Cree, Angela J; Morgan, B Paul; Lotery, Andrew J

    2013-08-01

    To investigate whether modification of liver complement factor H (CFH) production, by alteration of liver CFH Y402H genotype through liver transplantation (LT), influences the development of age-related macular degeneration (AMD). Multicenter, cross-sectional study. We recruited 223 Western European patients ≥ 55 years old who had undergone LT ≥ 5 years previously. We determined AMD status using a standard grading system. Recipient CFH Y402H genotype was obtained from DNA extracted from recipient blood samples. Donor CFH Y402H genotype was inferred from recipient plasma CFH Y402H protein allotype, measured using enzyme-linked immunosorbent assays. This approach was verified by genotyping donor tissue from a subgroup of patients. Systemic complement activity was ascertained by measuring levels of plasma complement proteins using an enzyme-linked immunosorbent assay, including substrates (C3, C4), activation products (C3a, C4a, and terminal complement complex), and regulators (total CFH, C1 inhibitor). We evaluated AMD status and recipient and donor CFH Y402H genotype. In LT patients, AMD was associated with recipient CFH Y402H genotype (P = 0.036; odds ratio [OR], 1.6; 95% confidence interval [CI], 1.0-2.4) but not with donor CFH Y402H genotype (P = 0.626), after controlling for age, sex, smoking status, and body mass index. Recipient plasma CFH Y402H protein allotype predicted donor CFH Y402H genotype with 100% accuracy (n = 49). Plasma complement protein or activation product levels were similar in LT patients with and without AMD. Compared with previously reported prevalence figures (Rotterdam Study), LT patients demonstrated a high prevalence of both AMD (64.6% vs 37.1%; OR, 3.09; Pproduction. In addition, AMD is not associated with systemic complement activity in LT patients. These findings suggest that local intraocular complement activity is of greater importance in AMD pathogenesis. The high AMD prevalence observed in LT patients may be associated with

  13. Inhibition of the classical pathway of the complement system by saliva of Amblyomma cajennense (Acari: Ixodidae).

    Science.gov (United States)

    Franco, Paula F; Silva, Naylene C S; Fazito do Vale, Vladimir; Abreu, Jéssica F; Santos, Vânia C; Gontijo, Nelder F; Valenzuela, Jesus G; Pereira, Marcos H; Sant'Anna, Mauricio R V; Gomes, Alessandra P S; Araujo, Ricardo N

    2016-05-01

    Inhibition of the complement system during and after haematophagy is of utmost importance for tick success in feeding and tick development. The role of such inhibition is to minimise damage to the intestinal epithelium as well as avoiding inflammation and opsonisation of salivary molecules at the bite site. Despite its importance, the salivary anti-complement activity has been characterised only in species belonging to the Ixodes ricinus complex which saliva is able to inhibit the alternative and lectin pathways. Little is known about this activity in other species of the Ixodidae family. Thus, the aim of this study was to describe the inhibition of the classical pathway of the complement system by the saliva of Amblyomma cajennense at different stages of the haematophagy. The A. cajennense saliva and salivary gland extract (SGE) were able to inhibit the complement classical pathway through haemolytic assays with higher activity observed when saliva was used. The anti-complement activity is present in the salivary glands of starving females and also in females throughout the whole feeding process, with significant higher activity soon after tick detachment. The SGE activity from both females fed on mice or horses had no significant correlation (p > 0.05) with tick body weight. The pH found in the intestinal lumen of A. cajennense was 8.04 ± 0.08 and haemolytic assays performed at pH 8.0 showed activation of the classical pathway similarly to what occurs at pH 7.4. Consequently, inhibition could be necessary to protect the tick enterocytes. Indeed, the inhibition observed by SGE was higher in pH 8.0 in comparison to pH 7.4 reinforcing the role of saliva in protecting the intestinal cells. Further studies should be carried out in order to identify the inhibitor molecule and characterise its inhibition mechanism. Copyright © 2016 Elsevier Inc. All rights reserved.

  14. Statistics of Shared Components in Complex Component Systems

    Science.gov (United States)

    Mazzolini, Andrea; Gherardi, Marco; Caselle, Michele; Cosentino Lagomarsino, Marco; Osella, Matteo

    2018-04-01

    Many complex systems are modular. Such systems can be represented as "component systems," i.e., sets of elementary components, such as LEGO bricks in LEGO sets. The bricks found in a LEGO set reflect a target architecture, which can be built following a set-specific list of instructions. In other component systems, instead, the underlying functional design and constraints are not obvious a priori, and their detection is often a challenge of both scientific and practical importance, requiring a clear understanding of component statistics. Importantly, some quantitative invariants appear to be common to many component systems, most notably a common broad distribution of component abundances, which often resembles the well-known Zipf's law. Such "laws" affect in a general and nontrivial way the component statistics, potentially hindering the identification of system-specific functional constraints or generative processes. Here, we specifically focus on the statistics of shared components, i.e., the distribution of the number of components shared by different system realizations, such as the common bricks found in different LEGO sets. To account for the effects of component heterogeneity, we consider a simple null model, which builds system realizations by random draws from a universe of possible components. Under general assumptions on abundance heterogeneity, we provide analytical estimates of component occurrence, which quantify exhaustively the statistics of shared components. Surprisingly, this simple null model can positively explain important features of empirical component-occurrence distributions obtained from large-scale data on bacterial genomes, LEGO sets, and book chapters. Specific architectural features and functional constraints can be detected from occurrence patterns as deviations from these null predictions, as we show for the illustrative case of the "core" genome in bacteria.

  15. Statistics of Shared Components in Complex Component Systems

    Directory of Open Access Journals (Sweden)

    Andrea Mazzolini

    2018-04-01

    Full Text Available Many complex systems are modular. Such systems can be represented as “component systems,” i.e., sets of elementary components, such as LEGO bricks in LEGO sets. The bricks found in a LEGO set reflect a target architecture, which can be built following a set-specific list of instructions. In other component systems, instead, the underlying functional design and constraints are not obvious a priori, and their detection is often a challenge of both scientific and practical importance, requiring a clear understanding of component statistics. Importantly, some quantitative invariants appear to be common to many component systems, most notably a common broad distribution of component abundances, which often resembles the well-known Zipf’s law. Such “laws” affect in a general and nontrivial way the component statistics, potentially hindering the identification of system-specific functional constraints or generative processes. Here, we specifically focus on the statistics of shared components, i.e., the distribution of the number of components shared by different system realizations, such as the common bricks found in different LEGO sets. To account for the effects of component heterogeneity, we consider a simple null model, which builds system realizations by random draws from a universe of possible components. Under general assumptions on abundance heterogeneity, we provide analytical estimates of component occurrence, which quantify exhaustively the statistics of shared components. Surprisingly, this simple null model can positively explain important features of empirical component-occurrence distributions obtained from large-scale data on bacterial genomes, LEGO sets, and book chapters. Specific architectural features and functional constraints can be detected from occurrence patterns as deviations from these null predictions, as we show for the illustrative case of the “core” genome in bacteria.

  16. Coherent systems with multistate components

    International Nuclear Information System (INIS)

    Caldarola, L.

    1980-01-01

    The basic rules of the Boolean algebra with restrictions on variables are briefly recalled. This special type of Boolean algebra allows one to handle fault trees of systems made of multistate (two or more than two states) components. Coherent systems are defined in the case of multistate components. This definition is consistent with that originally suggested by Barlow in the case of binary (two states) components. The basic properties of coherence are described and discussed. Coherent Boolean functions are also defined. It is shown that these functions are irredundant, that is they have only one base which is at the same time complete and irredundant. However, irredundant functions are not necessarily coherent. Finally a simplified algorithm for the calculation of the base of a coherent function is described. In the case that the function is not coherent, the algorithm can be used to reduce the size of the normal disjunctive form of the function. This in turn eases the application of the Nelson algorithm to calculate the complete base of the function. The simplified algorithm has been built in the computer program MUSTAFA-1. In a sample case the use of this algorithm caused a reduction of the CPU time by a factor of about 20. (orig.)

  17. Component Control System for a Vehicle

    Science.gov (United States)

    Fraser-Chanpong, Nathan (Inventor); Spain, Ivan (Inventor); Dawson, Andrew D. (Inventor); Bluethmann, William J. (Inventor); Lee, Chunhao J. (Inventor); Vitale, Robert L. (Inventor); Guo, Raymond (Inventor); Waligora, Thomas M. (Inventor); Akinyode, Akinjide Akinniyi (Inventor); Reed, Ryan M. (Inventor)

    2016-01-01

    A vehicle includes a chassis, a modular component, and a central operating system. The modular component is supported by the chassis. The central operating system includes a component control system, a primary master controller, and a secondary master controller. The component control system is configured for controlling the modular component. The primary and secondary master controllers are in operative communication with the component control system. The primary and secondary master controllers are configured to simultaneously transmit commands to the component control system. The component control system is configured to accept commands from the secondary master controller only when a fault occurs in the primary master controller.

  18. Modelization of cooling system components

    Energy Technology Data Exchange (ETDEWEB)

    Copete, Monica; Ortega, Silvia; Vaquero, Jose Carlos; Cervantes, Eva [Westinghouse Electric (Spain)

    2010-07-01

    In the site evaluation study for licensing a new nuclear power facility, the criteria involved could be grouped in health and safety, environment, socio-economics, engineering and cost-related. These encompass different aspects such as geology, seismology, cooling system requirements, weather conditions, flooding, population, and so on. The selection of the cooling system is function of different parameters as the gross electrical output, energy consumption, available area for cooling system components, environmental conditions, water consumption, and others. Moreover, in recent years, extreme environmental conditions have been experienced and stringent water availability limits have affected water use permits. Therefore, modifications or alternatives of current cooling system designs and operation are required as well as analyses of the different possibilities of cooling systems to optimize energy production taking into account water consumption among other important variables. There are two basic cooling system configurations: - Once-through or Open-cycle; - Recirculating or Closed-cycle. In a once-through cooling system (or open-cycle), water from an external water sources passes through the steam cycle condenser and is then returned to the source at a higher temperature with some level of contaminants. To minimize the thermal impact to the water source, a cooling tower may be added in a once-through system to allow air cooling of the water (with associated losses on site due to evaporation) prior to returning the water to its source. This system has a high thermal efficiency, and its operating and capital costs are very low. So, from an economical point of view, the open-cycle is preferred to closed-cycle system, especially if there are no water limitations or environmental restrictions. In a recirculating system (or closed-cycle), cooling water exits the condenser, goes through a fixed heat sink, and is then returned to the condenser. This configuration

  19. Component codification and identification systems

    International Nuclear Information System (INIS)

    Pannenbaecker, K.

    1977-01-01

    The lecture covers the codification in power stations during the erection phase and commercial operation phase. A diagram gives a survey. There are three basic-codifications for application; 1) Kraftwerk-Kennzeichen-System (KKS) for marking each component in orientated systems, for marking electrical orientated positions in cubicals, switch gears etc. and for marking rooms in buildings; 2) Ordnungssystem (OS) for cost calculation and ordering; 3) Unterlagenarten-Schluessel (UAS) for letters, reports etc. and for documentation. The OS is developed on the principle of cost account number and is therefore close to the organization of each supplier and his special form of design and constrution. KKS has only to mark hardware. Therefore all German owners, consultants, authorities and suppliers develop KKS together and conform to it in DIN 407119. (ORU) [de

  20. Cooling system for auxiliary reactor component

    International Nuclear Information System (INIS)

    Fujihira, Tomoko.

    1991-01-01

    A cooling system for auxiliary reactor components comprises three systems, that is, two systems of reactor component cooling water systems (RCCW systems) and a high pressure component cooling water system (HPCCW system). Connecting pipelines having partition valves are intervened each in a cooling water supply pipeline to an emmergency component of each of the RCCW systems, a cooling water return pipeline from the emmergency component of each of the RCCW systems, a cooling water supply pipeline to each of the emmergency components of one of the RCCW system and the HPCCW system and a cooling water return pipeline from each of the emmergency components of one of the RCCW system and the HPCCW system. With such constitution, cooling water can be supplied also to the emmergency components in the stand-by system upon periodical inspection or ISI, thereby enabling to improve the backup performance of the emmergency cooling system. (I.N.)

  1. Molluskan Hemocyanins Activate the Classical Pathway of the Human Complement System through Natural Antibodies.

    Science.gov (United States)

    Pizarro-Bauerle, Javier; Maldonado, Ismael; Sosoniuk-Roche, Eduardo; Vallejos, Gerardo; López, Mercedes N; Salazar-Onfray, Flavio; Aguilar-Guzmán, Lorena; Valck, Carolina; Ferreira, Arturo; Becker, María Inés

    2017-01-01

    Molluskan hemocyanins are enormous oxygen-carrier glycoproteins that show remarkable immunostimulatory properties when inoculated in mammals, such as the generation of high levels of antibodies, a strong cellular reaction, and generation of non-specific antitumor immune responses in some types of cancer, particularly for superficial bladder cancer. These proteins have the ability to bias the immune response toward a T h 1 phenotype. However, despite all their current uses with beneficial clinical outcomes, a clear mechanism explaining these properties is not available. Taking into account reports of natural antibodies against the hemocyanin of the gastropod Megathura crenulata [keyhole limpet hemocyanin (KLH)] in humans as well as other vertebrate species, we report here for the first time, the presence, in sera from unimmunized healthy donors, of antibodies recognizing, in addition to KLH, two other hemocyanins from gastropods with documented immunomodulatory capacities: Fisurella latimarginata hemocyanin (FLH) and Concholepas concholepas hemocyanin (CCH). Through an ELISA screening, we found IgM and IgG antibodies reactive with these hemocyanins. When the capacity of these antibodies to bind deglycosylated hemocyanins was studied, no decreased interaction was detected. Moreover, in the case of FLH, deglycosylation increased antibody binding. We evaluated through an in vitro complement deposition assay whether these antibodies activated the classical pathway of the human complement system. The results showed that all three hemocyanins and their deglycosylated counterparts elicited this activation, mediated by C1 binding to immunoglobulins. Thus, this work contributes to the understanding on how the complement system could participate in the immunostimulatory properties of hemocyanins, through natural, complement-activating antibodies reacting with these proteins. Although a role for carbohydrates cannot be completely ruled out, in our experimental setting

  2. Reactivity of alpha 1-antitrypsin mutants against proteolytic enzymes of the kallikrein-kinin, complement, and fibrinolytic systems

    NARCIS (Netherlands)

    Patston, P.A.; Roodi, N.; Schifferli, J.A.; Bischoff, Rainer; Courtney, M.; Schapira, M.

    1990-01-01

    Increased extracellular proteolysis because of unregulated activation of blood coagulation, complement, and fibrinolysis is observed in thrombosis, shock, and inflammation. In the present study, we have examined whether the plasma kallikrein-kinin system, the classical pathway of complement, and the

  3. Sodium polyanethole sulfonate as an inhibitor of activation of complement function in blood culture systems

    DEFF Research Database (Denmark)

    Palarasah, Yaseelan; Skjoedt, Mikkel-Ole; Vitved, Lars

    2010-01-01

    complement activation pathways: the classical, alternative, and lectin pathways, respectively. Inhibition of complement activity by SPS is caused by a blocking of complement activation and is not a result of complement consumption. The classical pathway is inhibited at SPS concentrations greater than 0.1 mg...... findings also open up the possibility of a new assay for the assessment of the functional capacity of the lectin complement pathway....

  4. The magnet components database system

    International Nuclear Information System (INIS)

    Baggett, M.J.; Leedy, R.; Saltmarsh, C.; Tompkins, J.C.

    1990-01-01

    The philosophy, structure, and usage MagCom, the SSC magnet components database, are described. The database has been implemented in Sybase (a powerful relational database management system) on a UNIX-based workstation at the Superconducting Super Collider Laboratory (SSCL); magnet project collaborators can access the database via network connections. The database was designed to contain the specifications and measured values of important properties for major materials, plus configuration information (specifying which individual items were used in each cable, coil, and magnet) and the test results on completed magnets. These data will facilitate the tracking and control of the production process as well as the correlation of magnet performance with the properties of its constituents. 3 refs., 10 figs

  5. The magnet components database system

    International Nuclear Information System (INIS)

    Baggett, M.J.; Leedy, R.; Saltmarsh, C.; Tompkins, J.C.

    1990-01-01

    The philosophy, structure, and usage of MagCom, the SSC magnet components database, are described. The database has been implemented in Sybase (a powerful relational database management system) on a UNIX-based workstation at the Superconducting Super Collider Laboratory (SSCL); magnet project collaborators can access the database via network connections. The database was designed to contain the specifications and measured values of important properties for major materials, plus configuration information (specifying which individual items were used in each cable, coil, and magnet) and the test results on completed magnets. The data will facilitate the tracking and control of the production process as well as the correlation of magnet performance with the properties of its constituents. 3 refs., 9 figs

  6. The lectin complement pathway serine proteases (MASPs) represent a possible crossroad between the coagulation and complement systems in thromboinflammation

    DEFF Research Database (Denmark)

    Kozarcanin, H; Lood, C; Fog, Lea Munthe

    2016-01-01

    by AT during clotting without the assistance of heparin. In all other cases the MASPs were, as previously reported, inactivated by C1-INH. In systemic lupus erythematosus patients with thrombotic disease and in polytrauma patients, the levels of activated MASP-1 and MASP-2 in complex with both AT and C1-INH...

  7. Coupling component systems towards systems of systems

    OpenAIRE

    Autran , Frédéric; Auzelle , Jean-Philippe; Cattan , Denise; Garnier , Jean-Luc; Luzeaux , Dominique; Mayer , Frédérique; Peyrichon , Marc; Ruault , Jean-René

    2008-01-01

    International audience; Systems of systems (SoS) are a hot topic in our "fully connected global world". Our aim is not to provide another definition of what SoS are, but rather to focus on the adequacy of reusing standard system architecting techniques within this approach in order to improve performance, fault detection and safety issues in large-scale coupled systems that definitely qualify as SoS, whatever the definition is. A key issue will be to secure the availability of the services pr...

  8. Trypanosoma cruzi Evades the Complement System as an Efficient Strategy to Survive in the Mammalian Host: The Specific Roles of Host/Parasite Molecules and Trypanosoma cruzi Calreticulin

    Directory of Open Access Journals (Sweden)

    Galia Ramírez-Toloza

    2017-09-01

    Full Text Available American Trypanosomiasis is an important neglected reemerging tropical parasitism, infecting about 8 million people worldwide. Its agent, Trypanosoma cruzi, exhibits multiple mechanisms to evade the host immune response and infect host cells. An important immune evasion strategy of T. cruzi infective stages is its capacity to inhibit the complement system activation on the parasite surface, avoiding opsonizing, immune stimulating and lytic effects. Epimastigotes, the non-infective form of the parasite, present in triatomine arthropod vectors, are highly susceptible to complement-mediated lysis while trypomastigotes, the infective form, present in host bloodstream, are resistant. Thus T. cruzi susceptibility to complement varies depending on the parasite stage (amastigote, trypomastigotes or epimastigote and on the T. cruzi strain. To avoid complement-mediated lysis, T. cruzi trypomastigotes express on the parasite surface a variety of complement regulatory proteins, such as glycoprotein 58/68 (gp58/68, T. cruzi complement regulatory protein (TcCRP, trypomastigote decay-accelerating factor (T-DAF, C2 receptor inhibitor trispanning (CRIT and T. cruzi calreticulin (TcCRT. Alternatively, or concomitantly, the parasite captures components with complement regulatory activity from the host bloodstream, such as factor H (FH and plasma membrane-derived vesicles (PMVs. All these proteins inhibit different steps of the classical (CP, alternative (AP or lectin pathways (LP. Thus, TcCRP inhibits the CP C3 convertase assembling, gp58/68 inhibits the AP C3 convertase, T-DAF interferes with the CP and AP convertases assembling, TcCRT inhibits the CP and LP, CRIT confers ability to resist the CP and LP, FH is used by trypomastigotes to inhibit the AP convertases and PMVs inhibit the CP and LP C3 convertases. Many of these proteins have similar molecular inhibitory mechanisms. Our laboratory has contributed to elucidate the role of TcCRT in the host

  9. Zonulin as prehaptoglobin2 regulates lung permeability and activates the complement system

    OpenAIRE

    Rittirsch, Daniel; Flierl, Michael A.; Nadeau, Brian A.; Day, Danielle E.; Huber-Lang, Markus S.; Grailer, Jamison J.; Zetoune, Firas S.; Andjelkovic, Anuska V.; Fasano, Alessio; Ward, Peter A.

    2013-01-01

    Zonulin is a protein involved in the regulation of tight junctions (TJ) in epithelial or endothelial cells. Zonulin is known to affect TJ in gut epithelial cells, but little is known about its influences in other organs. Prehaptoglobin2 has been identified as zonulin and is related to serine proteases (MASPs, C1qrs) that activate the complement system. The current study focused on the role of zonulin in development of acute lung injury (ALI) in C57BL/6 male mice following intrapulmonary depos...

  10. Correlation of systemic lupus erythematosus disease activity with classical complement (CH50 function and related protein levels

    Directory of Open Access Journals (Sweden)

    Salesi M

    2008-09-01

    Full Text Available "nBackground: The components of the classical complement pathway play an important role in the pathogenesis of systemic lupus erythematosus (SLE and are reportedly useful biomarkers of disease activity. In this study, we evaluate disease activity, complement function (total hemolytic complement, CH50 and complement protein levels (C3, C4, C3d, C4d, SC5b-9, comparing the results of patients with active disease versus those with inactive disease."n"nMethods: This cross-sectional study included 78 hospitalized women with SLE, 24 of whom were in the active group, with SLE disease activity indexes (SLEDAI.2K of >6, and 54 in the inactive group, with SLEDAI.2K of ≤6. Serum CH50 was measured using a red blood cell hemolytic assay. C3 and C4 levels were determined by nephlometry and plasma levels of C3d, C4d, SC5b-9 by ELISA. The data were statistically analyzed using SPSS."n"nResults: The mean (±standard error C4d levels of the inactive group were significantly higher than those of the active group (23.39±1.1µg/ml and 16.9±1.6µg/ml, respectively; p=0.003. There was also a significant correlation between C3 and C4 levels (p=0.807. The mean values of the other proteins (C3, C4, CH50, SC5b-9, and C3d circulating immune complex concentrations were not significantly different between the inactive group vs. the active group: 89.35±6.8 vs. 85.54±7.6mg/dl, 18.33±2.3 vs. 20.45±2.4mg/dl, 149.03±4.3 vs. 157±4.3U, 1414.4±114.94 vs. 1471.1±216.9ng/ml, 9.43±0.96 vs. 13.31±3.16µgEq/ml, respectively (p>0.05."n"nConclusions: According to our results, C4d levels may be used as a biomarker of disease activity. The significant correlation between C3 and C4 may confirm the activity of the classical pathway in SLE patients."n"nKeywords: Systemic lupus erythematosus, CH50, C3, C4, C3d, C4d, SC5b-9, inactive, flare.

  11. Proteolysis of the heavy chain of major histocompatibility complex class I antigens by complement component C1s

    DEFF Research Database (Denmark)

    Eriksson, H; Nissen, Mogens Holst

    1990-01-01

    weights of the fragments are in agreement with the cleavage located in the area between the disulphide loops of the alpha 2-and alpha 3-domains of the heavy chain. In addition human C1s complement is able to cleave H-2 antigens from mouse in a similar fashion but not rat MHC class I antigen or mouse MHC...... class II antigen (I-Ad). Mouse MHC class I antigen-specific determinants could also be detected in supernatant from mouse spleen cells incubated with C1r and C1s. These results indicate the presence in the body fluids of a non-membrane-bound soluble form of the alpha 1-and alpha 2-domains which...

  12. Inhibition of the complement system by saliva of Anopheles (Nyssorhynchus) aquasalis.

    Science.gov (United States)

    Mendes-Sousa, Antonio Ferreira; Vale, Vladimir Fazito; Queiroz, Daniel Costa; Pereira-Filho, Adalberto Alves; da Silva, Naylene Carvalho Sales; Koerich, Leonardo Barbosa; Moreira, Luciano Andrade; Pereira, Marcos Horácio; Sant'Anna, Maurício Roberto; Araújo, Ricardo Nascimento; Andersen, John; Valenzuela, Jesus Gilberto; Gontijo, Nelder Figueiredo

    2018-01-01

    Anopheline mosquitoes are vectors of malaria parasites. Their saliva contains anti-hemostatic and immune-modulator molecules that favor blood feeding and parasite transmission. In this study, we describe the inhibition of the alternative pathway of the complement system (AP) by Anopheles aquasalis salivary gland extracts (SGE). According to our results, the inhibitor present in SGE acts on the initial step of the AP blocking deposition of C3b on the activation surfaces. Properdin, which is a positive regulatory molecule of the AP, binds to SGE. When SGE was treated with an excess of properdin, it was unable to inhibit the AP. Through SDS-PAGE analysis, A. aquasalis presented a salivary protein with the same molecular weight as recombinant complement inhibitors belonging to the SG7 family described in the saliva of other anopheline species. At least some SG7 proteins bind to properdin and are AP inhibitors. Searching for SG7 proteins in the A. aquasalis genome, we retrieved a salivary protein that shared an 85% identity with albicin, which is the salivary alternative pathway inhibitor from A. albimanus. This A. aquasalis sequence was also very similar (81% ID) to the SG7 protein from A. darlingi, which is also an AP inhibitor. Our results suggest that the salivary complement inhibitor from A. aquasalis is an SG7 protein that can inhibit the AP by binding to properdin and abrogating its stabilizing activity. Albicin, which is the SG7 from A. albimanus, can directly inhibit AP convertase. Given the high similarity of SG7 proteins, the SG7 from A. aquasalis may also directly inhibit AP convertase in the absence of properdin. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Zonulin as prehaptoglobin2 regulates lung permeability and activates the complement system.

    Science.gov (United States)

    Rittirsch, Daniel; Flierl, Michael A; Nadeau, Brian A; Day, Danielle E; Huber-Lang, Markus S; Grailer, Jamison J; Zetoune, Firas S; Andjelkovic, Anuska V; Fasano, Alessio; Ward, Peter A

    2013-06-15

    Zonulin is a protein involved in the regulation of tight junctions (TJ) in epithelial or endothelial cells. Zonulin is known to affect TJ in gut epithelial cells, but little is known about its influences in other organs. Prehaptoglobin2 has been identified as zonulin and is related to serine proteases (MASPs, C1qrs) that activate the complement system. The current study focused on the role of zonulin in development of acute lung injury (ALI) in C57BL/6 male mice following intrapulmonary deposition of IgG immune complexes. A zonulin antagonist (AT-1001) and a related peptide with permeability agonist activities (AT-1002) were employed and given intratracheally or intravenously. Also, zonulin was blocked in lung with a neutralizing antibody. In a dose-dependent manner, AT-1001 or zonulin neutralizing antibody attenuated the intensity of ALI (as quantitated by albumin leak, neutrophil accumulation, and proinflammatory cytokines). A similar pattern was found using the bacterial lipopolysaccharide model of ALI. Using confocal microscopy on sections of injured lungs, staining patterns for TJ proteins were discontinuous, reduced, and fragmented. As expected, the leak of blood products into the alveolar space confirmed the passage of 3 and 20 kDa dextran, and albumin. In contrast to AT-1001, application of the zonulin agonist AT-1002 intensified ALI. Zonulin both in vitro and in vivo induced generation of complement C3a and C5a. Collectively, these data suggest that zonulin facilitates development of ALI both by enhancing albumin leak and complement activation as well as increased buildup of neutrophils and cytokines during development of ALI.

  14. Acquired partial lipodystrophy and C3 glomerulopathy: Dysregulation of the complement system as a common mechanism

    Directory of Open Access Journals (Sweden)

    Fernando Corvillo

    2018-05-01

    Full Text Available The activation of the alternative pathway of the complement is involved in the development of several renal diseases, such as atypical haemolytic uraemic syndrome and C3 glomerulopathy. In C3 glomerulopathy, a high percentage of patients have circulating levels of the autoantibody called C3NeF, which causes systemic dysregulation of the complement system. In some cases, the presence of this antibody has been related with abnormalities of adipose tissue, causing acquired partial lipodystrophy (Barraquer–Simons syndrome. Acquired partial lipodystrophy is an extremely rare disorder affecting the distribution of subcutaneous adipose tissue and that mainly onsets during childhood. These patients, in addition to possibly presenting with all the metabolic disorders associated with the adipose tissue defect, present with C3 hypocomplementemia and C3NeF and 25% have developed C3 glomerulopathy. Although it has been known for some time how the dysregulation of the complement system affects the kidneys, it remains unknown how it exactly affects adipose tissue; nevertheless, the relationship is quite clear. In this paper, we describe the connection between the complement system with the biology of the adipose tissue and its pathogenesis reflected from acquired partial lipodystrophy. Resumen: La activación de la vía alternativa del complemento interviene en el desarrollo de varias enfermedades renales, como el síndrome hemolítico urémico atípico o la glomerulopatía C3. En esta última enfermedad un elevado porcentaje de los pacientes presentan niveles circulantes de un autoanticuerpo denominado C3NeF, causante de la desregulación del complemento a nivel sistémico. En ciertos casos, la presencia de este anticuerpo se asocia con alteraciones en el tejido adiposo, causando lipodistrofia parcial adquirida (síndrome de Barraquer-Simons, una enfermedad ultra-rara que afecta a la distribución del tejido adiposo subcutáneo y que comienza principalmente

  15. Molecular cloning, structural analysis and expression of complement component Bf/C2 genes in the nurse shark, Ginglymostoma cirratum.

    Science.gov (United States)

    Shin, Dong-Ho; Webb, Barbara; Nakao, Miki; Smith, Sylvia L

    2007-01-01

    Factor B and C2 are serine proteases that provide the catalytic subunits of C3 and C5 convertases of the alternative (AP) and classical (CP) complement pathways. Two Bf/C2 cDNAs, GcBf/C2-1 and -2 (previously referred to as nsBf/C2-A and nsBf/C2-B), were isolated from the nurse shark, Ginglymostoma cirratum. GcBf/C2-1 and -2 are 3364 and 3082bp in length and encode a leader peptide, three CCPs, one VWFA, the serine protease domain and have a putative factor D/C1s/MASP cleavage site. Southern blots show that there might be up to two Bf/C2-like genes for each of the two GcBf/C2 isoforms. GcBf/C2-1 and -2 are constitutively expressed, albeit at different levels, in all nine tissues examined. Expression in erythrocytes is a novel finding. Structural analysis has revealed that the localization of glycosylation sites in the SP domain of both putative proteins indicates that the molecular organization of the shark molecules is more like C2 than factor B. Phylogenetic analysis indicates that GcBf/C2-1 and -2 and TrscBf of Triakis scyllia (another shark species) originated from a common ancestor and share a remote ancestor with Bf and C2 of mammals and bony fish.

  16. Interaction of extremophilic archaeal viruses with human and mouse complement system and viral biodistribution in mice

    DEFF Research Database (Denmark)

    Wu, Linping; Uldahl, Kristine Buch; Chen, Fangfang

    2017-01-01

    -dependent manner, but C3 deficiency has no overall effect on viral clearance by organs of the reticuloendothelial system on intravenous injection. However, splenic deposition was significantly higher in C3 knockout animals compared with the corresponding wild type mice. We discuss the potential application......Archaeal viruses offer exceptional biophysical properties for modification and exploration of their potential in bionanotechnology, bioengineering and nanotherapeutic developments. However, the interaction of archaeal viruses with elements of the innate immune system has not been explored, which...... surface, but factor H deposition is purely C3-dependent. This suggests that unlike some virulent pathogens Sulfolobus monocaudavirus 1 does not acquire factor H for protection. Complement activation with Sulfolobus monocaudavirus 1 also proceeds in murine sera through MBL-A/C as well as factor D...

  17. Fanconi anemia: correlating central nervous system malformations and genetic complementation groups.

    Science.gov (United States)

    Johnson-Tesch, Benjamin A; Gawande, Rakhee S; Zhang, Lei; MacMillan, Margaret L; Nascene, David R

    2017-06-01

    Congenital central nervous system abnormalities in children with Fanconi anemia are poorly characterized, especially with regard to specific genetic complementation groups. To characterize the impact of genetic complementation groups on central nervous system anatomy. Through chart review we identified 36 patients with Fanconi anemia with available brain MRIs at the University of Minnesota (average age, 11.3 years; range, 1-43 years; M:F=19:17), which we reviewed and compared to 19 age- and sex-matched controls (average age, 7.9 years; range, 2-18 years; M:F=9:10). Genotypic information was available for 27 patients (15 FA-A, 2 FA-C, 3 FA-G, and 7 FA-D1 [biallelic mutations in BRCA2 gene]). Of the 36 patients, 61% had at least one congenital central nervous system or skull base abnormality. These included hypoplastic clivus (n=12), hypoplastic adenohypophysis (n=11), platybasia (n=8), pontocerebellar hypoplasia (n=7), isolated pontine hypoplasia (n=4), isolated vermis hypoplasia (n=3), and ectopic neurohypophysis (n=6). Average pituitary volume was significantly less in patients with Fanconi anemia (PFanconi anemia patients (P=0.006), but the basal angle of those with FA-D1 was not significantly different from controls (P=0.239). Clivus length was less in the Fanconi anemia group (P=0.002), but significance was only observed in the FA-D1 subgroup (PFanconi anemia have higher incidences of ectopic neurohypophysis, adenohypophysis hypoplasia, platybasia and other midline central nervous system skull base posterior fossa abnormalities than age- and sex-matched controls. Patients with posterior fossa abnormalities, including pontocerebellar hypoplasia, are more likely to have biallelic BRCA2 mutations.

  18. Fanconi anemia: correlating central nervous system malformations and genetic complementation groups

    International Nuclear Information System (INIS)

    Johnson-Tesch, Benjamin A.; Gawande, Rakhee S.; Nascene, David R.; Zhang, Lei; MacMillan, Margaret L.

    2017-01-01

    Congenital central nervous system abnormalities in children with Fanconi anemia are poorly characterized, especially with regard to specific genetic complementation groups. To characterize the impact of genetic complementation groups on central nervous system anatomy. Through chart review we identified 36 patients with Fanconi anemia with available brain MRIs at the University of Minnesota (average age, 11.3 years; range, 1-43 years; M:F=19:17), which we reviewed and compared to 19 age- and sex-matched controls (average age, 7.9 years; range, 2-18 years; M:F=9:10). Genotypic information was available for 27 patients (15 FA-A, 2 FA-C, 3 FA-G, and 7 FA-D1 [biallelic mutations in BRCA2 gene]). Of the 36 patients, 61% had at least one congenital central nervous system or skull base abnormality. These included hypoplastic clivus (n=12), hypoplastic adenohypophysis (n=11), platybasia (n=8), pontocerebellar hypoplasia (n=7), isolated pontine hypoplasia (n=4), isolated vermis hypoplasia (n=3), and ectopic neurohypophysis (n=6). Average pituitary volume was significantly less in patients with Fanconi anemia (P<0.0001) than in controls. Basal angle was significantly greater in Fanconi anemia patients (P=0.006), but the basal angle of those with FA-D1 was not significantly different from controls (P=0.239). Clivus length was less in the Fanconi anemia group (P=0.002), but significance was only observed in the FA-D1 subgroup (P<0.0001). Of the seven patients meeting criteria for pontocerebellar hypoplasia, six belonged to the FA-D1 group. Patients with Fanconi anemia have higher incidences of ectopic neurohypophysis, adenohypophysis hypoplasia, platybasia and other midline central nervous system skull base posterior fossa abnormalities than age- and sex-matched controls. Patients with posterior fossa abnormalities, including pontocerebellar hypoplasia, are more likely to have biallelic BRCA2 mutations. (orig.)

  19. Fanconi anemia: correlating central nervous system malformations and genetic complementation groups

    Energy Technology Data Exchange (ETDEWEB)

    Johnson-Tesch, Benjamin A. [University of Minnesota, Department of Radiology, Minneapolis, MN (United States); Gawande, Rakhee S.; Nascene, David R. [University of Minnesota, Department of Radiology, Neuroradiology Section, Minneapolis, MN (United States); Zhang, Lei [University of Minnesota, Biostatistical Design and Analysis Centre, Minneapolis, MN (United States); MacMillan, Margaret L. [University of Minnesota, Blood and Marrow Transplant Program, Department of Pediatrics, Minneapolis, MN (United States)

    2017-06-15

    Congenital central nervous system abnormalities in children with Fanconi anemia are poorly characterized, especially with regard to specific genetic complementation groups. To characterize the impact of genetic complementation groups on central nervous system anatomy. Through chart review we identified 36 patients with Fanconi anemia with available brain MRIs at the University of Minnesota (average age, 11.3 years; range, 1-43 years; M:F=19:17), which we reviewed and compared to 19 age- and sex-matched controls (average age, 7.9 years; range, 2-18 years; M:F=9:10). Genotypic information was available for 27 patients (15 FA-A, 2 FA-C, 3 FA-G, and 7 FA-D1 [biallelic mutations in BRCA2 gene]). Of the 36 patients, 61% had at least one congenital central nervous system or skull base abnormality. These included hypoplastic clivus (n=12), hypoplastic adenohypophysis (n=11), platybasia (n=8), pontocerebellar hypoplasia (n=7), isolated pontine hypoplasia (n=4), isolated vermis hypoplasia (n=3), and ectopic neurohypophysis (n=6). Average pituitary volume was significantly less in patients with Fanconi anemia (P<0.0001) than in controls. Basal angle was significantly greater in Fanconi anemia patients (P=0.006), but the basal angle of those with FA-D1 was not significantly different from controls (P=0.239). Clivus length was less in the Fanconi anemia group (P=0.002), but significance was only observed in the FA-D1 subgroup (P<0.0001). Of the seven patients meeting criteria for pontocerebellar hypoplasia, six belonged to the FA-D1 group. Patients with Fanconi anemia have higher incidences of ectopic neurohypophysis, adenohypophysis hypoplasia, platybasia and other midline central nervous system skull base posterior fossa abnormalities than age- and sex-matched controls. Patients with posterior fossa abnormalities, including pontocerebellar hypoplasia, are more likely to have biallelic BRCA2 mutations. (orig.)

  20. Gyrotron: an ECH system component

    International Nuclear Information System (INIS)

    Loring, C.M.; Eason, H.O.; Kimrey, H.D.; White, T.L.; Jory, H.R.; Evans, S.J.

    1981-01-01

    The gyrotron, or electron-cyclotron maser, in the form of a gyromonotron, is being developed as a source of millimeter wave energy for fusion plasma heating. The characteristics of this high power, high efficiency electron tube are described in terms of the requirements for the beam power supply system, the mechanical support system, the cooling system, the focusing and tuning magnets, and the waveguide system. Requirements of power level and transmission efficiency dictate the use of oversize waveguide. The implications, both to the user and to the interaction mechanisms in the gyrotron, of the use of oversize waveguide are treated. The effects of variations of various operating parameters upon the gyrotron's power output and stability are also discussed. Data from gyrotron development and system operation are used where appropriate

  1. Two component systems: physiological effect of a third component.

    Directory of Open Access Journals (Sweden)

    Baldiri Salvado

    Full Text Available Signal transduction systems mediate the response and adaptation of organisms to environmental changes. In prokaryotes, this signal transduction is often done through Two Component Systems (TCS. These TCS are phosphotransfer protein cascades, and in their prototypical form they are composed by a kinase that senses the environmental signals (SK and by a response regulator (RR that regulates the cellular response. This basic motif can be modified by the addition of a third protein that interacts either with the SK or the RR in a way that could change the dynamic response of the TCS module. In this work we aim at understanding the effect of such an additional protein (which we call "third component" on the functional properties of a prototypical TCS. To do so we build mathematical models of TCS with alternative designs for their interaction with that third component. These mathematical models are analyzed in order to identify the differences in dynamic behavior inherent to each design, with respect to functionally relevant properties such as sensitivity to changes in either the parameter values or the molecular concentrations, temporal responsiveness, possibility of multiple steady states, or stochastic fluctuations in the system. The differences are then correlated to the physiological requirements that impinge on the functioning of the TCS. This analysis sheds light on both, the dynamic behavior of synthetically designed TCS, and the conditions under which natural selection might favor each of the designs. We find that a third component that modulates SK activity increases the parameter space where a bistable response of the TCS module to signals is possible, if SK is monofunctional, but decreases it when the SK is bifunctional. The presence of a third component that modulates RR activity decreases the parameter space where a bistable response of the TCS module to signals is possible.

  2. The lectin pathway of complement

    DEFF Research Database (Denmark)

    Ballegaard, Vibe Cecilie Diederich; Haugaard, Anna Karen; Garred, P

    2014-01-01

    The pattern recognition molecules of the lectin complement pathway are important components of the innate immune system with known functions in host-virus interactions. This paper summarizes current knowledge of how these intriguing molecules, including mannose-binding lectin (MBL), Ficolin-1, -2......-1, -2 and -3 and CL-11 could have similar functions in HIV infection as the ficolins have been shown to play a role in other viral infections, and CL-11 resembles MBL and the ficolins in structure and binding capacity.......The pattern recognition molecules of the lectin complement pathway are important components of the innate immune system with known functions in host-virus interactions. This paper summarizes current knowledge of how these intriguing molecules, including mannose-binding lectin (MBL), Ficolin-1, -2...

  3. Triatoma infestans Calreticulin: Gene Cloning and Expression of a Main Domain That Interacts with the Host Complement System.

    Science.gov (United States)

    Weinberger, Katherine; Collazo, Norberto; Aguillón, Juan Carlos; Molina, María Carmen; Rosas, Carlos; Peña, Jaime; Pizarro, Javier; Maldonado, Ismael; Cattan, Pedro E; Apt, Werner; Ferreira, Arturo

    2017-02-08

    Triatoma infestans is an important hematophagous vector of Chagas disease, a neglected chronic illness affecting approximately 6 million people in Latin America. Hematophagous insects possess several molecules in their saliva that counteract host defensive responses. Calreticulin (CRT), a multifunctional protein secreted in saliva, contributes to the feeding process in some insects. Human CRT (HuCRT) and Trypanosoma cruzi CRT (TcCRT) inhibit the classical pathway of complement activation, mainly by interacting through their central S domain with complement component C1. In previous studies, we have detected CRT in salivary gland extracts from T. infestans We have called this molecule TiCRT. Given that the S domain is responsible for C1 binding, we have tested its role in the classical pathway of complement activation in vertebrate blood. We have cloned and characterized the complete nucleotide sequence of CRT from T. infestans , and expressed its S domain. As expected, this S domain binds to human C1 and, as a consequence, it inhibits the classical pathway of complement, at its earliest stage of activation, namely the generation of C4b. Possibly, the presence of TiCRT in the salivary gland represents an evolutionary adaptation in hematophagous insects to control a potential activation of complement proteins, present in the massive blood meal that they ingest, with deleterious consequences at least on the anterior digestive tract of these insects. © The American Society of Tropical Medicine and Hygiene.

  4. Different host complement systems and their interactions with saliva from Lutzomyia longipalpis (Diptera, Psychodidae and Leishmania infantum promastigotes.

    Directory of Open Access Journals (Sweden)

    Antonio Ferreira Mendes-Sousa

    Full Text Available BACKGROUND: Lutzomyia longipalpis is the vector of Leishmania infantum in the New World, and its saliva inhibits classical and alternative human complement system pathways. This inhibition is important in protecting the insect´s midgut from damage by the complement. L. longipalpis is a promiscuous blood feeder and must be protected against its host's complement. The objective of this study was to investigate the action of salivary complement inhibitors on the sera of different host species, such as dogs, guinea pigs, rats and chickens, at a pH of 7.4 (normal blood pH and 8.15 (the midgut pH immediately after a blood meal. We also investigated the role of the chicken complement system in Leishmania clearance in the presence and absence of vector saliva. RESULTS: The saliva was capable of inhibiting classical pathways in dogs, guinea pigs and rats at both pHs. The alternative pathway was not inhibited except in dogs at a pH of 8.15. The chicken classical pathway was inhibited only by high concentrations of saliva and it was better inhibited by the midgut contents of sand flies. Neither the saliva nor the midgut contents had any effect on the avian alternative pathway. Fowl sera killed L. infantum promastigotes, even at a low concentration (2%, and the addition of L. longipalpis saliva did not protect the parasites. The high body temperature of chickens (40°C had no effect on Leishmania viability during our assays. CONCLUSION: Salivary inhibitors act in a species-specific manner. It is important to determine their effects in the natural hosts of Leishmania infantum because they act on canid and rodent complements but not on chickens (which do not harbour the parasite. Moreover, we concluded that the avian complement system is the probable mechanism through which chickens eliminate Leishmania and that their high body temperature does not influence this parasite.

  5. Different host complement systems and their interactions with saliva from Lutzomyia longipalpis (Diptera, Psychodidae) and Leishmania infantum promastigotes.

    Science.gov (United States)

    Mendes-Sousa, Antonio Ferreira; Nascimento, Alexandre Alves Sousa; Queiroz, Daniel Costa; Vale, Vladimir Fazito; Fujiwara, Ricardo Toshio; Araújo, Ricardo Nascimento; Pereira, Marcos Horácio; Gontijo, Nelder Figueiredo

    2013-01-01

    Lutzomyia longipalpis is the vector of Leishmania infantum in the New World, and its saliva inhibits classical and alternative human complement system pathways. This inhibition is important in protecting the insect´s midgut from damage by the complement. L. longipalpis is a promiscuous blood feeder and must be protected against its host's complement. The objective of this study was to investigate the action of salivary complement inhibitors on the sera of different host species, such as dogs, guinea pigs, rats and chickens, at a pH of 7.4 (normal blood pH) and 8.15 (the midgut pH immediately after a blood meal). We also investigated the role of the chicken complement system in Leishmania clearance in the presence and absence of vector saliva. The saliva was capable of inhibiting classical pathways in dogs, guinea pigs and rats at both pHs. The alternative pathway was not inhibited except in dogs at a pH of 8.15. The chicken classical pathway was inhibited only by high concentrations of saliva and it was better inhibited by the midgut contents of sand flies. Neither the saliva nor the midgut contents had any effect on the avian alternative pathway. Fowl sera killed L. infantum promastigotes, even at a low concentration (2%), and the addition of L. longipalpis saliva did not protect the parasites. The high body temperature of chickens (40°C) had no effect on Leishmania viability during our assays. Salivary inhibitors act in a species-specific manner. It is important to determine their effects in the natural hosts of Leishmania infantum because they act on canid and rodent complements but not on chickens (which do not harbour the parasite). Moreover, we concluded that the avian complement system is the probable mechanism through which chickens eliminate Leishmania and that their high body temperature does not influence this parasite.

  6. Using hydropower to complement wind energy: a hybrid system to provide firm power

    Energy Technology Data Exchange (ETDEWEB)

    Jaramillo, O.A.; Borja, M.A.; Huacuz, J.M. [Instituto de Investigaciones Electricas, Morelos (Mexico). Energias No Convencionales

    2004-09-01

    This paper presents a theoretical study of how wind power can be complemented by hydropower. A conceptual framework is provided for a hybrid power station that produces constant power output without the intermittent fluctuations inherent when using wind power. Two hypothetical facilities are considered as case studies. One of them is a hydropower plant located on the ''Presidente Benito Juarez'' dam in Jalapa del Marques, Oaxaca, Mexico. The other hypothetical facility is a wind farm located near ''La Venta's', an area in Juchitan, Oaxaca, Mexico. The wind-hydro-power system is a combined wind and hydro power plant in a region that is rich in both resources. The model shows that the hybrid plant could provide close to 20 MW of firm power to the electrical distribution system. On a techno-economic basis, we obtain the levelized production cost of the hybrid system. Taking into account two different discount rates of 7% and 10%, figures for levelized production cost are developed. (author)

  7. Effects of partial replacement of fish meal by yeast hydrolysate on complement system and stress resistance in juvenile Jian carp (Cyprinus carpio var. Jian).

    Science.gov (United States)

    Yuan, Xiang-Yang; Liu, Wen-Bin; Liang, Chao; Sun, Cun-Xin; Xue, Yun-Fei; Wan, Zu-De; Jiang, Guang-Zhen

    2017-08-01

    A 10-week feeding trial was carried out to investigate the effects of dietary fish meal replacement by yeast hydrolysate (YH) on growth performance, complement system and stress resistance of juvenile Jian carp (Cyprinus carpio var. Jian) (initial average weight 19.44 ± 0.06 g). In the study, there were five groups: one control group was fed with a basal diet (YH0), and four treatment groups were fed with dietary fish meal replaced by 1% YH (YH1), 3% (YH3), 5% (YH5) and 7% (YH7), respectively. Each group had four replicates. At the end of feeding trial, twelve fish from each group (three fish per replicate) were randomly selected for assessing the growth and immunity. Meanwhile, 20 fish per replicate were injected by Aeromonas hydrophila. The results showed that (1) Replacement levels of YH significantly affected the growth of the fish with the highest values of weight gain (WG) occurred in fish fed YH3 diet. However, no significant difference in feed conversion ratios (FCR) was observed among all groups. (2) Pre-stressed plasma lysozyme activity, total protein and albumin contents and complement component 3 (C3) and complement component 4 (C4) levels of fish fed YH3 diet were significantly higher than those of fish fed YH0 diet. However, post-stressed immune parameters of fish in all groups were significantly lower. (3) There was a trend that the expression levels of the complement-related genes (c1r/s-A, c4-1, c3-H1, c5-1, fb/c2-A, mbl-2 and masp) initially increased and then decreased except mbl-2 and masp, with the maximum values observed in fish fed YH3 diet. Before stress, the expression levels of the inflammation-related genes (alp, il-1β and tnf-α) in the hepatopancreas and spleen of fish fed YH1 diet and YH7 diet were significant higher than that of fish fed YH0 diet. After stress, no significant difference in the expression levels of those genes was observed among all groups. These results indicated that FM replacement by YH could improve growth

  8. Hybrid solar lighting distribution systems and components

    Science.gov (United States)

    Muhs, Jeffrey D [Lenoir City, TN; Earl, Dennis D [Knoxville, TN; Beshears, David L [Knoxville, TN; Maxey, Lonnie C [Powell, TN; Jordan, John K [Oak Ridge, TN; Lind, Randall F [Lenoir City, TN

    2011-07-05

    A hybrid solar lighting distribution system and components having at least one hybrid solar concentrator, at least one fiber receiver, at least one hybrid luminaire, and a light distribution system operably connected to each hybrid solar concentrator and each hybrid luminaire. A controller operates all components.

  9. CL-L1 and CL-K1 and other complement associated pattern recognition molecules in systemic lupus erythematosus

    DEFF Research Database (Denmark)

    Troldborg, Anne; Thiel, Steffen; Jensen, Lisbeth

    2015-01-01

    The objective of this study was to explore the involvement of collectin liver 1 (CL-L1) and collectin kidney 1 (CL-K1) and other pattern recognition molecules (PRMs) of the lectin pathway of the complement system in a cross-sectional cohort of systemic lupus erythematosus (SLE) patients...

  10. Oral vaccination with heat inactivated Mycobacterium bovis activates the complement system to protect against tuberculosis.

    Directory of Open Access Journals (Sweden)

    Beatriz Beltrán-Beck

    Full Text Available Tuberculosis (TB remains a pandemic affecting billions of people worldwide, thus stressing the need for new vaccines. Defining the correlates of vaccine protection is essential to achieve this goal. In this study, we used the wild boar model for mycobacterial infection and TB to characterize the protective mechanisms elicited by a new heat inactivated Mycobacterium bovis vaccine (IV. Oral vaccination with the IV resulted in significantly lower culture and lesion scores, particularly in the thorax, suggesting that the IV might provide a novel vaccine for TB control with special impact on the prevention of pulmonary disease, which is one of the limitations of current vaccines. Oral vaccination with the IV induced an adaptive antibody response and activation of the innate immune response including the complement component C3 and inflammasome. Mycobacterial DNA/RNA was not involved in inflammasome activation but increased C3 production by a still unknown mechanism. The results also suggested a protective mechanism mediated by the activation of IFN-γ producing CD8+ T cells by MHC I antigen presenting dendritic cells (DCs in response to vaccination with the IV, without a clear role for Th1 CD4+ T cells. These results support a role for DCs in triggering the immune response to the IV through a mechanism similar to the phagocyte response to PAMPs with a central role for C3 in protection against mycobacterial infection. Higher C3 levels may allow increased opsonophagocytosis and effective bacterial clearance, while interfering with CR3-mediated opsonic and nonopsonic phagocytosis of mycobacteria, a process that could be enhanced by specific antibodies against mycobacterial proteins induced by vaccination with the IV. These results suggest that the IV acts through novel mechanisms to protect against TB in wild boar.

  11. OCSEGen: Open Components and Systems Environment Generator

    Science.gov (United States)

    Tkachuk, Oksana

    2014-01-01

    To analyze a large system, one often needs to break it into smaller components.To analyze a component or unit under analysis, one needs to model its context of execution, called environment, which represents the components with which the unit interacts. Environment generation is a challenging problem, because the environment needs to be general enough to uncover unit errors, yet precise enough to make the analysis tractable. In this paper, we present a tool for automated environment generation for open components and systems. The tool, called OCSEGen, is implemented on top of the Soot framework. We present the tool's current support and discuss its possible future extensions.

  12. Digital Components in Swedish NPP Power Systems

    International Nuclear Information System (INIS)

    Karlsson, Mattias; Eriksson, Tage

    2015-01-01

    Swedish nuclear power plants have over the last 20 years of operation modernised or exchanged several systems and components of the electrical power system. Within these works, new components based on digital technology have been employed in order to realize functionality that was previously achieved by using electro-mechanical or analogue technology. Components and systems such as relay protection, rectifiers, inverters, variable speed drives and diesel-generator sets are today equipped with digital components. Several of the systems and components fulfil functions with a safety-role in the NPP. Recently, however, a number of incidents have occurred which highlight deficiencies in the design or HMI of the equipment, which warrants questions whether there are generic problems with some applications of digital components that needs to be addressed. The use of digital components has presented cost effective solutions, or even the only available solution on the market enabling a modernisation. The vast majority of systems using digital components have been operating without problems and often contribute to improved safety but the challenge of non-detectable, or non-identifiable, failure modes remain. In this paper, the extent to which digital components are used in Swedish NPP power systems will be presented including a description of typical applications. Based on data from maintenance records and fault reports, as well as interviews with designers and maintenance personnel, the main areas where problems have been encountered and where possible risks have been identified will be described. The paper intends to investigate any 'tell-tales' that could give signals of unwanted behaviour. Furthermore, particular benefits experienced by using digital components will be highlighted. The paper will also discuss the safety relevance of these findings and suggest measures to improve safety in the application of digital components in power systems. (authors)

  13. Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.

    Science.gov (United States)

    Nissilä, Eija; Douillard, François P; Ritari, Jarmo; Paulin, Lars; Järvinen, Hanna M; Rasinkangas, Pia; Haapasalo, Karita; Meri, Seppo; Jarva, Hanna; de Vos, Willem M

    2017-01-01

    Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin. However, in some cases, Lactobacillus spp. have been implicated in bacteremia. The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG. Blood cultures from bacteremic patients were collected at the Helsinki University Hospital laboratory in 2005-2011 and L. rhamnosus strains were isolated and characterized by genomic sequencing. The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC). Binding of complement regulators factor H and C4bp was also determined using radioligand assays. Furthermore, the isolated strains were evaluated for their ability to aggregate platelets and to form biofilms in vitro. Genomic comparison between the clinical L. rhamnosus strains showed them to be clearly different from L. rhamnosus GG and to cluster in two distinct lineages. All L. rhamnosus strains activated complement in serum and none of them bound complement regulators. Four out of 16 clinical blood isolates induced platelet aggregation and/or formed more biofilms than L. rhamnosus GG, which did not display platelet aggregation activity nor showed strong biofilm formation. These findings suggest that clinical L. rhamnosus isolates show considerable heterogeneity but are clearly different from L. rhamnosus GG at the genomic level. All L. rhamnosus strains are still normally recognized by the human complement system.

  14. Complement Test

    Science.gov (United States)

    ... Salicylates Semen Analysis Serotonin Serum Free Light Chains Sex Hormone Binding Globulin (SHBG) Shiga toxin-producing Escherichia ... and forming complexes that respond to infections, non-self tissues (transplants), dead cells ... KJ. Complement determinations in human disease. Ann Allergy Asthma Immunol . 2004; ...

  15. The effect of storage temperature on the biological activity of extracellular vesicles for the complement system.

    Science.gov (United States)

    Park, Sang June; Jeon, Hyungtaek; Yoo, Seung-Min; Lee, Myung-Shin

    2018-05-10

    Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions. In this study, we investigated various conditions to identify the best conditions to store EVs with functional stability for 25 d. Furthermore, the correlation between the function and other characteristics of EVs, including the expression of EV markers, size distribution, and particle number, were also analyzed. Our results demonstrated that storage temperature is an important factor to maintain the activity of EVs and would be useful information for basic research and clinical application using EVs.

  16. C3 Glomerulopathy and Atypical Hemolytic Uremic Syndrome: Two Important Manifestations of Complement System Dysfunction

    Directory of Open Access Journals (Sweden)

    Ravneet Bajwa

    2018-02-01

    Full Text Available The advances in our understanding of the alternative pathway have emphasized that uncontrolled hyperactivity of this pathway causes 2 distinct disorders that adversely impact the kidney. In the so-called atypical hemolytic uremic syndrome (aHUS, renal dysfunction occurs along with thrombocytopenia, anemia, and target organ injury to multiple organs, most commonly the kidney. On the other hand, in the so-termed C3 glomerulopathy, kidney involvement is not associated with thrombocytopenia, anemia, or other system involvement. In this report, we present 2 cases of alternative pathway dysfunction. The 60-year-old female patient had biopsy-proven C3 glomerulopathy, while the 32-year-old female patient was diagnosed with aHUS based on renal dysfunction, thrombocytopenia, anemia, and normal ADAMTS-13 level. The aHUS patient was successfully treated with the monoclonal antibody (eculizumab for complement blockade. The patient with C3 glomerulopathy did not receive the monoclonal antibody. In this patient, management focused on blood pressure and proteinuria control with an angiotensin-converting enzyme inhibitor. This article focuses on the clinical differences, pathophysiology, and treatment of aHUS and C3 glomerulopathy.

  17. Structured Performance Analysis for Component Based Systems

    OpenAIRE

    Salmi , N.; Moreaux , Patrice; Ioualalen , M.

    2012-01-01

    International audience; The Component Based System (CBS) paradigm is now largely used to design software systems. In addition, performance and behavioural analysis remains a required step for the design and the construction of efficient systems. This is especially the case of CBS, which involve interconnected components running concurrent processes. % This paper proposes a compositional method for modeling and structured performance analysis of CBS. Modeling is based on Stochastic Well-formed...

  18. Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings

    DEFF Research Database (Denmark)

    Andersen, T.E.; Kolmos, H.J.; Palarasah, Yaseelan

    2011-01-01

    In the current study we investigate the activation of blood complement on medical device silicone rubber and present a plasma polymerized vinyl pyrrolidone (ppVP) coating which strongly decreases surface-activation of the blood complement system. We show that uncoated silicone and polystyrene...... surface. The ppVP surface is furthermore characterized physically and chemically using scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR), which indicates preservation of chemical functionality by the applied plasma process. Overall, the pp...

  19. Systems with randomly failing repairable components

    DEFF Research Database (Denmark)

    Der Kiureghian, Armen; Ditlevsen, Ove Dalager; Song, Junho

    2005-01-01

    Closed-form expressions are derived for the steady-state availability, mean rate of failure, mean duration of downtime and reliability of a general system with randomly and independently failing repairable components. Component failures are assumed to be homogeneous Poisson events in time and rep...

  20. Effects of coagulation temperature on measurements of complement function in serum samples from patients with systemic lupus erythematosus

    DEFF Research Database (Denmark)

    Baatrup, G; Sturfelt, G; Junker, A

    1992-01-01

    Blood samples from 15 patients with systemic lupus erythematosus (SLE) and 15 healthy blood donors were allowed to coagulate for one hour at room temperature, followed by one hour at 4 or 37 degrees C. The complement activity of the serum samples was assessed by three different functional assays...

  1. Complement component 3 (C3)

    Science.gov (United States)

    ... the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic ... proteins may go down. For example, people with active lupus erythematosus may have lower-than-normal levels ...

  2. Human complement component C3

    DEFF Research Database (Denmark)

    Behrendt, N

    1985-01-01

    The two common genetic variants of human C3, C3 S and C3 F, were purified and characterized by SDS-PAGE, agarose gel electrophoresis, isoelectric focusing and amino acid analysis. The difference in electrophoretic mobility between the two variants was conserved after purification, and by isoelect......The two common genetic variants of human C3, C3 S and C3 F, were purified and characterized by SDS-PAGE, agarose gel electrophoresis, isoelectric focusing and amino acid analysis. The difference in electrophoretic mobility between the two variants was conserved after purification......, and by isoelectric focusing of the hemolytically active proteins, pI values of 5.86 and 5.81 were determined for C3 S and C3 F, respectively. Any difference in amino acid composition was too small to be detected by amino acid analysis, and the two proteins had the same molecular weight as determined by SDS-PAGE....

  3. Molecular cloning of the alpha subunit of complement component C8 (CpC8α) of whitespotted bamboo shark (Chiloscyllium plagiosum).

    Science.gov (United States)

    Wang, Ying; Zhang, Mengmeng; Wang, Conghui; Ye, Boping; Hua, Zichun

    2013-12-01

    Complement-mediated cytolysis is the important effect of immune response, which results from the assembly of terminal complement components (C5b-9). Among them, α subunit of C8 (C8α) is the first protein that traverses the lipid bilayer, and then initiates the recruitment of C9 molecules to form pore on target membranes. In this article, a full-length cDNA of C8α (CpC8α) is identified from the whitespotted bamboo shark (Chiloscyllium plagiosum) by RACE. The CpC8α cDNA is 2183 bp in length, encoding a protein of 591 amino acids. The deduced CpC8α exhibits 89%, 49% and 44% identity with nurse shark, frog and human orthologs, respectively. Sequence alignment indicates that the C8α is well conserved during the evolution process from sharks to mammals, with the same modular architecture as well as the identical cysteine composition in the mature protein. Phylogenetic analysis places CpC8α and nurse shark C8α in cartilaginous fish clade, in parallel with the teleost taxa, to form the C8α cluster with higher vertebrates. Hydrophobicity analysis also indicates a similar hydrophobicity of CpC8α to mammals. Finally, expression analysis revealed CpC8α transcripts were constitutively highly expressed in shark liver, with much less expression in other tissues. The well conserved structure and properties suggests an analogous function of CpC8α to mammalian C8α, though it remains to be confirmed by further study. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. Solid State Lighting Reliability Components to Systems

    CERN Document Server

    Fan, XJ

    2013-01-01

    Solid State Lighting Reliability: Components to Systems begins with an explanation of the major benefits of solid state lighting (SSL) when compared to conventional lighting systems including but not limited to long useful lifetimes of 50,000 (or more) hours and high efficacy. When designing effective devices that take advantage of SSL capabilities the reliability of internal components (optics, drive electronics, controls, thermal design) take on critical importance. As such a detailed discussion of reliability from performance at the device level to sub components is included as well as the integrated systems of SSL modules, lamps and luminaires including various failure modes, reliability testing and reliability performance. This book also: Covers the essential reliability theories and practices for current and future development of Solid State Lighting components and systems Provides a systematic overview for not only the state-of-the-art, but also future roadmap and perspectives of Solid State Lighting r...

  5. Hot gas path component cooling system

    Science.gov (United States)

    Lacy, Benjamin Paul; Bunker, Ronald Scott; Itzel, Gary Michael

    2014-02-18

    A cooling system for a hot gas path component is disclosed. The cooling system may include a component layer and a cover layer. The component layer may include a first inner surface and a second outer surface. The second outer surface may define a plurality of channels. The component layer may further define a plurality of passages extending generally between the first inner surface and the second outer surface. Each of the plurality of channels may be fluidly connected to at least one of the plurality of passages. The cover layer may be situated adjacent the second outer surface of the component layer. The plurality of passages may be configured to flow a cooling medium to the plurality of channels and provide impingement cooling to the cover layer. The plurality of channels may be configured to flow cooling medium therethrough, cooling the cover layer.

  6. NHI Component Technical Readiness Evaluation System

    International Nuclear Information System (INIS)

    Sherman, S.; Wilson, Dane F.; Pawel, Steven J.

    2007-01-01

    A decision process for evaluating the technical readiness or maturity of components (i.e., heat exchangers, chemical reactors, valves, etc.) for use by the U.S. DOE Nuclear Hydrogen Initiative is described. This system is used by the DOE NHI to assess individual components in relation to their readiness for pilot-scale and larger-scale deployment and to drive the research and development work needed to attain technical maturity. A description of the evaluation system is provided, and examples are given to illustrate how it is used to assist in component R and D decisions.

  7. Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila.

    Directory of Open Access Journals (Sweden)

    Yunfei Dang

    Full Text Available The grass carp (Ctenopharyngodon idella is an important commercial farmed herbivorous fish species in China, but is susceptible to Aeromonas hydrophila infections. In the present study, we performed de novo RNA-Seq sequencing of spleen tissue from specimens of a disease-resistant family, which were given intra-peritoneal injections containing PBS with or without a dose of A. hydrophila. The fish were sampled from the control group at 0 h, and from the experimental group at 4, 8, 12, 24, 48 and 72 h. 122.18 million clean reads were obtained from the normalized cDNA libraries; these were assembled into 425,260 contigs and then 191,795 transcripts. Of those, 52,668 transcripts were annotated with the NCBI Nr database, and 41,347 of the annotated transcripts were assigned into 90 functional groups. 20,569 unigenes were classified into six main categories, including 38 secondary KEGG pathways. 2,992 unigenes were used in the analysis of differentially expressed genes (DEGs. 89 of the putative DEGs were related to the immune system and 41 of them were involved in the complement and coagulation cascades pathway. This study provides insights into the complement and complement-related pathways involved in innate immunity, through expression profile analysis of the genomic resources in C. idella. We conclude that complement and complement-related genes play important roles during defense against A. hydrophila infection. The immune response is activated at 4 h after the bacterial injections, indicating that the complement pathways are activated at the early stage of bacterial infection. The study has improved our understanding of the immune response mechanisms in C. idella to bacterial pathogens.

  8. Cardiac Sirt1 mediates the cardioprotective effect of caloric restriction by suppressing local complement system activation after ischemia-reperfusion.

    Science.gov (United States)

    Yamamoto, Tsunehisa; Tamaki, Kayoko; Shirakawa, Kohsuke; Ito, Kentaro; Yan, Xiaoxiang; Katsumata, Yoshinori; Anzai, Atsushi; Matsuhashi, Tomohiro; Endo, Jin; Inaba, Takaaki; Tsubota, Kazuo; Sano, Motoaki; Fukuda, Keiichi; Shinmura, Ken

    2016-04-15

    Caloric restriction (CR) confers cardioprotection against ischemia-reperfusion (I/R) injury. We previously found the essential roles of endothelial nitric oxide synthase in the development of CR-induced cardioprotection and Sirt1 activation during CR (Shinmura K, Tamaki K, Ito K, Yan X, Yamamoto T, Katsumata Y, Matsuhashi T, Sano M, Fukuda K, Suematsu M, Ishii I. Indispensable role of endothelial nitric oxide synthase in caloric restriction-induced cardioprotection against ischemia-reperfusion injury.Am J Physiol Heart Circ Physiol 308: H894-H903, 2015). However, the exact mechanism by which Sirt1 in cardiomyocytes mediates the cardioprotective effect of CR remains undetermined. We subjected cardiomyocyte-specific Sirt1 knockout (CM-Sirt1(-/-)) mice and the corresponding control mice to either 3-mo ad libitum feeding or CR (-40%). Isolated perfused hearts were subjected to 25-min global ischemia, followed by 60-min reperfusion. The recovery of left ventricle function after I/R was improved, and total lactate dehydrogenase release into the perfusate during reperfusion was attenuated in the control mice treated with CR, but a similar cardioprotective effect of CR was not observed in the CM-Sirt1(-/-)mice. The expression levels of cardiac complement component 3 (C3) at baseline and the accumulation of C3 and its fragments in the ischemia-reperfused myocardium were attenuated by CR in the control mice, but not in the CM-Sirt1(-/-)mice. Resveratrol treatment also attenuated the expression levels of C3 protein in cultured neonatal rat ventricular cardiomyocytes. Moreover, the degree of myocardial I/R injury in conventional C3 knockout (C3(-/-)) mice treated with CR was similar to that in the ad libitum-fed C3(-/-)mice, although the expression levels of Sirt1 were enhanced by CR. These results demonstrate that cardiac Sirt1 plays an essential role in CR-induced cardioprotection against I/R injury by suppressing cardiac C3 expression. This is the first report suggesting

  9. Functional genomics of tick thioester-containing proteins reveal the ancient origin of the complement system

    Czech Academy of Sciences Publication Activity Database

    Burešová, Veronika; Hajdušek, Ondřej; Franta, Zdeněk; Loosová, Gabriela; Grunclová, Lenka; Levashina, E.A.; Kopáček, Petr

    2011-01-01

    Roč. 3, č. 6 (2011), s. 623-630 ISSN 1662-811X R&D Projects: GA ČR GAP506/10/2136; GA MŠk(CZ) LC06009 Institutional research plan: CEZ:AV0Z60220518 Keywords : tick * thioester-containing proteins * complement Subject RIV: EC - Immunology Impact factor: 4.209, year: 2011

  10. Microsurgery simulation training system and set up: An essential system to complement every training programme.

    Science.gov (United States)

    Masud, Dhalia; Haram, Nadine; Moustaki, Margarita; Chow, Whitney; Saour, Samer; Mohanna, Pari Naz

    2017-07-01

    Microsurgical techniques are essential in plastic surgery; however, inconsistent training practices, acquiring these skills can be difficult. To address this, we designed a standardised laboratory-based microsurgical training programme, which allows trainees to develop their dexterity, visuospatial ability, operative flow and judgement as separate components. Thirty trainees completed an initial microsurgical anastomosis on a chicken femoral artery, assessed using the structured assessment of microsurgical skills (SAMS) method. The study group (n = 18) then completed a 3-month training programme, while the control group (n = 19) did not. A final anastomosis was completed by all trainees (n = 30). The study group had a significant improvement in the microsurgical technique, assessed using the SAMS score, when the initial and final scores were compared (Mean: 24 SAMS initial versus 49 SAMS final) (p group had a significantly lower rate of improvement (Mean: 23 SAMS initial versus 25 SAMS final). There was a significant difference between the final SAMS score of the study group and that of senior surgeons (Mean: 49 study final SAMS versus 58 senior SAMS). This validated programme is a safe, cost-effective and flexible method of allowing trainees to develop microsurgical skills in a non-pressurized environment. In addition, the objectified skills allow trainers to assess the trainees' level of proficiency before operating on patients. Copyright © 2017 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.

  11. Thermally activated, single component epoxy systems

    KAUST Repository

    Unruh, David A.

    2011-08-23

    A single component epoxy system in which the resin and hardener components found in many two-component epoxies are combined onto the same molecule is described. The single molecule precursor to the epoxy resin contains both multiple epoxide moieties and a diamine held latent by thermally degradable carbamate linkages. These bis-carbamate "single molecule epoxies" have an essentially infinite shelf life and access a significant range in curing temperatures related to the structure of the carbamate linkages used. © 2011 American Chemical Society.

  12. Thermally activated, single component epoxy systems

    KAUST Repository

    Unruh, David A.; Pastine, Stefan J.; Moreton, Jessica C.; Frechet, Jean

    2011-01-01

    A single component epoxy system in which the resin and hardener components found in many two-component epoxies are combined onto the same molecule is described. The single molecule precursor to the epoxy resin contains both multiple epoxide moieties and a diamine held latent by thermally degradable carbamate linkages. These bis-carbamate "single molecule epoxies" have an essentially infinite shelf life and access a significant range in curing temperatures related to the structure of the carbamate linkages used. © 2011 American Chemical Society.

  13. Crystallization and X-ray diffraction analysis of the complement component-3 (C3) inhibitory domain of Efb from Staphylococcus aureus

    International Nuclear Information System (INIS)

    Hammel, Michal; Ramyar, Kasra X.; Spencer, Charles T.; Geisbrecht, Brian V.

    2006-01-01

    The crystallization and results of multiwavelength anomalous diffraction studies of a recombinant C3-inhibitory fragment of Efb from S. aureus are reported. The extracellular fibrinogen-binding protein (Efb) of Staphylococcus aureus is a multifunctional virulence factor capable of potent inhibition of complement component-3 (C3) activity in addition to its previously described fibrinogen-binding properties. A truncated recombinant form of Efb (Efb-C) that binds C3 has been overexpressed and purified and has been crystallized using the hanging-drop vapor-diffusion technique. Crystals of native Efb-C grew in the tetragonal space group P4 3 (unit-cell parameters a = b = 59.53, c = 46.63 Å) with two molecules in the asymmetric unit and diffracted well beyond 1.25 Å limiting Bragg spacing. To facilitate de novo phasing of the Efb-C crystals, two independent site-directed mutants were engineered in which either residue Ile112 or Val140 was replaced with methionine and crystals isomorphous to those of native Efb-C were reproduced using a seleno-l-methionine-labeled form of each mutant protein. Multiwavelength anomalous diffraction (MAD) data were collected on both mutants and analyzed for their phasing power toward solution and refinement of a high-resolution Efb-C crystal structure

  14. Binding of complement proteins C1q and C4bp to serum amyloid P component (SAP) in solid contra liquid phase

    DEFF Research Database (Denmark)

    Sørensen, Inge Juul; Nielsen, EH; Andersen, Ove

    1996-01-01

    Serum amyloid P component (SAP), a member of the conserved pentraxin family of plasma proteins, binds calcium dependently to its ligands. The authors investigated SAPs interaction with the complement proteins C4b binding protein (C4bp) and C1q by ELISA, immunoelectrophoresis and electron microscopy....... Binding of these proteins to SAP was demonstrated when SAP was immobilized using F(ab')2 anti-SAP, but not when SAP reacted with these proteins in liquid phase; thus the binding to human SAP was markedly phase state dependent. Presaturation of solid phase SAP with heparin, which binds SAP with high...... affinity, did not interfere with the subsequent binding of C4bp or C1q to SAP. In contrast, collagen I and IV showed partial competition with the binding of C1q to SAP. Using fresh serum, immobilized native SAP bound C4bp whereas binding of C1q/C1 could not be demonstrated. Altogether the results indicate...

  15. Depletion of the Third Complement Component Ameliorates Age-Dependent Oxidative Stress and Positively Modulates Autophagic Activity in Aged Retinas in a Mouse Model

    Directory of Open Access Journals (Sweden)

    Dorota Rogińska

    2017-01-01

    Full Text Available The aim of the study was to investigate the influence of complement component C3 global depletion on the biological structure and function of the aged retina. In vivo morphology (OCT, electrophysiological function (ERG, and the expression of selected oxidative stress-, apoptosis-, and autophagy-related proteins were assessed in retinas of 12-month-old C3-deficient and WT mice. Moreover, global gene expression in retinas was analyzed by RNA arrays. We found that the absence of active C3 was associated with (1 alleviation of the age-dependent decrease in retinal thickness and gradual deterioration of retinal bioelectrical function, (2 significantly higher levels of antioxidant enzymes (catalase and glutathione reductase and the antiapoptotic survivin and Mcl-1/Bak dimer, (3 lower expression of the cellular oxidative stress marker—4HNE—and decreased activity of proapoptotic caspase-3, (4 ameliorated retinal autophagic activity with localization of ubiquitinated protein conjugates commonly along the retinal pigment epithelium (RPE layer, and (5 significantly increased expression of several gene sets associated with maintenance of the physiological functions of the neural retina. Our findings shed light on mechanisms of age-related retinal alterations by identifying C3 as a potential therapeutic target for retinal aging.

  16. Genetic susceptibility to chronic wasting disease in free-ranging white-tailed deer: complement component C1q and Prnp polymorphisms

    Science.gov (United States)

    Blanchong, Julie A.; Heisey, Dennis M.; Scribner, Kim T.; Libants, Scot V.; Johnson, Chad; Aiken, Judd M.; Langenberg, Julia A.; Samuel, Michael D.

    2009-01-01

    The genetic basis of susceptibility to chronic wasting disease (CWD) in free-ranging cervids is of great interest. Association studies of disease susceptibility in free-ranging populations, however, face considerable challenges including: the need for large sample sizes when disease is rare, animals of unknown pedigree create a risk of spurious results due to population admixture, and the inability to control disease exposure or dose. We used an innovative matched case–control design and conditional logistic regression to evaluate associations between polymorphisms of complement C1q and prion protein (Prnp) genes and CWD infection in white-tailed deer from the CWD endemic area in south-central Wisconsin. To reduce problems due to admixture or disease-risk confounding, we used neutral genetic (microsatellite) data to identify closely related CWD-positive (n = 68) and CWD-negative (n = 91) female deer to serve as matched cases and controls. Cases and controls were also matched on factors (sex, location, age) previously demonstrated to affect CWD infection risk. For Prnp, deer with at least one Serine (S) at amino acid 96 were significantly less likely to be CWD-positive relative to deer homozygous for Glycine (G). This is the first characterization of genes associated with the complement system in white-tailed deer. No tests for association between any C1q polymorphism and CWD infection were significant at p of CWD infection in deer with at least one Glycine (G) at amino acid 56 of the C1qC gene. While we documented numerous amino acid polymorphisms in C1q genes none appear to be strongly associated with CWD susceptibility.

  17. Component reliability criticality or importance metrics for systems with degrading components

    NARCIS (Netherlands)

    Peng, H.; Coit, D.W.; Feng, Q.

    2012-01-01

    This paper proposes two new importance measures: one new importance measure for systems with -independent degrading components, and another one for systems with -correlated degrading components. Importance measures in previous research are inadequate for systems with degrading components because

  18. Mesenchymal stromal cells engage complement and complement receptor bearing innate effector cells to modulate immune responses.

    Directory of Open Access Journals (Sweden)

    Guido Moll

    Full Text Available Infusion of human third-party mesenchymal stromal cells (MSCs appears to be a promising therapy for acute graft-versus-host disease (aGvHD. To date, little is known about how MSCs interact with the body's innate immune system after clinical infusion. This study shows, that exposure of MSCs to blood type ABO-matched human blood activates the complement system, which triggers complement-mediated lymphoid and myeloid effector cell activation in blood. We found deposition of complement component C3-derived fragments iC3b and C3dg on MSCs and fluid-phase generation of the chemotactic anaphylatoxins C3a and C5a. MSCs bound low amounts of immunoglobulins and lacked expression of complement regulatory proteins MCP (CD46 and DAF (CD55, but were protected from complement lysis via expression of protectin (CD59. Cell-surface-opsonization and anaphylatoxin-formation triggered complement receptor 3 (CD11b/CD18-mediated effector cell activation in blood. The complement-activating properties of individual MSCs were furthermore correlated with their potency to inhibit PBMC-proliferation in vitro, and both effector cell activation and the immunosuppressive effect could be blocked either by using complement inhibitor Compstatin or by depletion of CD14/CD11b-high myeloid effector cells from mixed lymphocyte reactions. Our study demonstrates for the first time a major role of the complement system in governing the immunomodulatory activity of MSCs and elucidates how complement activation mediates the interaction with other immune cells.

  19. Component aging evaluation with expert systems

    International Nuclear Information System (INIS)

    Wiesemann, J.S.; Maguire, H.T. Jr.

    1988-01-01

    The age degradation of components involves a complex relationship between a variety of variables. These relationships are typically modeled using probabilistic and deterministic analyses. These methods depend upon a formal understanding of the underlying degradation mechanisms and a database of experience which allows statistical analyses to extract numerical trends. At present, not all age degradation mechanisms are adequately modeled and available data for age degradation is in most cases insufficient. In addition, these methods tend to focus upon answers to isolated questions (e.g., What is the component failure rate?) rather than the more pertinent questions concerning operations and maintenance (e.g., should the component be replaced at the next outage). Fortunately, knowledge in the form of personal experience does exist which allows plant personnel to make decisions concerning operations and maintenance. This knowledge can be modeled using expert systems. This paper discusses CAGES (Component Aging Expert System). It combines expert rules (heuristics), probabilistic models, and deterministic models to make evaluations of component aging; predict the implications for component life extension, operational readiness, maintenance effectiveness, and safety, and make recommendations for maintenance and operation

  20. Diesel engine management systems and components

    CERN Document Server

    2014-01-01

    This reference book provides a comprehensive insight into todays diesel injection systems and electronic control. It focusses on minimizing emissions and exhaust-gas treatment. Innovations by Bosch in the field of diesel-injection technology have made a significant contribution to the diesel boom. Calls for lower fuel consumption, reduced exhaust-gas emissions and quiet engines are making greater demands on the engine and fuel-injection systems. Contents History of the diesel engine.- Areas of use for diesel engines.- Basic principles of the diesel engine.- Fuels: Diesel fuel.- Fuels: Alternative fuels.- Cylinder-charge control systems.- Basic principles of diesel fuel-injection.- Overview of diesel fuel-injection systems.- Fuel supply to the low pressure stage.- Overview of discrete cylinder systems.- Unit injector system.- Unit pump system.- Overview of common-rail systems.- High pressure components of the common-rail system.- Injection nozzles.- Nozzle holders.- High pressure lines.- Start assist systems.-...

  1. Comprehensive approach to study complement C4 in systemic lupus erythematosus: Gene polymorphisms, protein levels and functional activity.

    Science.gov (United States)

    Tsang-A-Sjoe, M W P; Bultink, I E M; Korswagen, L A; van der Horst, A; Rensink, I; de Boer, M; Hamann, D; Voskuyl, A E; Wouters, D

    2017-12-01

    Genetic variation of the genes encoding complement component C4 is strongly associated with systemic lupus erythematosus (SLE), a chronic multi-organ auto-immune disease. This study examined C4 and its isotypes on a genetic, protein, and functional level in 140 SLE patients and 104 healthy controls. Gene copy number (GCN) variation, silencing CT-insertion, and the retroviral HERV-K(C4) insertion) were analyzed with multiplex ligation-dependent probe amplification. Increased susceptibility to SLE was found for low GCN (≪2) of C4A. Serositis was the only clinical manifestation associated with low C4A GCN. One additional novel silencing mutation in the C4A gene was found by Sanger sequencing. This mutation causes a premature stop codon in exon 11. Protein concentrations of C4 isoforms C4A and C4B were determined with ELISA and were significantly lower in SLE patients compared to healthy controls. To study C4 isotypes on a functional level, a new C4 assay was developed, which distinguishes C4A from C4B by its binding capacity to amino or hydroxyl groups, respectively. This assay showed high correlation with ELISA and detected crossing over of Rodgers and Chido antigens in 3.2% (8/244) of individuals. The binding capacity of available C4 to its substrates was unaffected in SLE. Our study provides, for the first time, a complete overview of C4 in SLE from genetic variation to binding capacity using a novel test. As this test detects crossing over of Rodgers and Chido antigens, it will allow for more accurate measurement of C4 in future studies. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Perspective : component tracking on the Nova system

    International Nuclear Information System (INIS)

    MacDonald, S.

    1999-01-01

    The issue of introducing Component Tracking as a service to natural gas producers, shippers and straddle plant operators was discussed. Approximately 39 companies in the industry were contacted by consultants at Nova Gas Transmission in an effort to assess if introducing this service would add value to individual producers. The numerous implications that may have to be dealt with if Component Tracking is introduced were also described. Component Tracking would provide an equitable approach to the allocation of molecules in the gas stream, and could provide producers with the ability to avoid capital outlay in field plants by alternatively contracting for recovery of the liquids at the straddle plants. Component Tracking is to be voluntary and each shipper would be able to decide whether to utilize the service at each of their receipt points onto the Nova system

  3. Integrating the pastoral component in agricultural systems

    Directory of Open Access Journals (Sweden)

    Paulo César de Faccio Carvalho

    2018-03-01

    Full Text Available ABSTRACT This paper aims to discuss the impact of the introduction of pastures and grazing animals in agricultural systems. For the purposes of this manuscript, we focus on within-farm integrated crop-livestock systems (ICLS, typical of Southern Brazil. These ICLS are designed to create and enhance the synergisms and emergent properties have arisen from agricultural areas where livestock activities are integrated with crops. We show that the introduction of the crop component will affect less the preceding condition than the introduction of the livestock component. While the introduction of crops in pastoral systems represents increasing diversity of the plant component, the introduction of animals would represent the entry of new flows and interactions within the system. Thus, given the new complexity levels achieved from the introduction of grazing, the probability of arising emergent properties is theoretically much higher. However, grazing management is vital in determining the success or failure of such initiative. The grazing intensity practiced during the pasture phase would affect the canopy structure and the forage availability to animals. In adequate and moderate grazing intensities, it is possible to affirm that livestock combined with crops (ICLS has a potential positive impact. As important as the improvements that grazing animals can generate to the soil-plant components, the economic resilience remarkably increases when pasture rotations are introduced compared with purely agriculture systems, particularly in climate-risk situations. Thus, the integration of the pastoral component can enhance the sustainable intensification of food production, but it modifies simple, pure agricultural systems into more complex and knowledge-demanding production systems.

  4. Complement component 1, q subcomponent binding protein (C1QBP) in lipid rafts mediates hepatic metastasis of pancreatic cancer by regulating IGF-1/IGF-1R signaling.

    Science.gov (United States)

    Shi, Haojun; Fang, Winston; Liu, Minda; Fu, Deliang

    2017-10-01

    Pancreatic cancer shows a remarkable predilection for hepatic metastasis. Complement component 1, q subcomponent binding protein (C1QBP) can mediate growth factor-induced cancer cell chemotaxis and distant metastasis by activation of receptor tyrosine kinases. Coincidentally, insulin-like growth factor-1 (IGF-1) derived from the liver and cancer cells itself has been recognized as a critical inducer of hepatic metastasis. However, the mechanism underlying IGF-1-dependent hepatic metastasis of pancreatic cancer, in which C1QBP may be involved, remains unknown. In the study, we demonstrated a significant association between C1QBP expression and hepatic metastasis in patients with pancreatic cancer. IGF-1 induced the translocation of C1QBP from cytoplasm to lipid rafts and further drove the formation of CD44 variant 6 (CD44v6)/C1QBP complex in pancreatic cancer cells. C1QBP interacting with CD44v6 in lipid rafts promoted phosphorylation of IGF-1R and thus activated downstream PI3K and MAPK signaling pathways which mediated metastatic potential of pancreatic cancer cells including proliferation, apoptosis, invasion, adhesion and energy metabolism. Furthermore, C1QBP knockdown suppressed hepatic metastasis of pancreatic cancer cells in nude mice. We therefore conclude that C1QBP in lipid rafts serves a key regulator of IGF-1/IGF-1R-induced hepatic metastasis from pancreatic cancer. Our findings about C1QBP in lipid rafts provide a novel strategy to block IGF-1/IGF-1R signaling in pancreatic cancer and a reliable premise for more efficient combined modality therapies. © 2017 UICC.

  5. BUSINESS PROCESS MANAGEMENT SYSTEMS TECHNOLOGY COMPONENTS ANALYSIS

    Directory of Open Access Journals (Sweden)

    Andrea Giovanni Spelta

    2007-05-01

    Full Text Available The information technology that supports the implementation of the business process management appproach is called Business Process Management System (BPMS. The main components of the BPMS solution framework are process definition repository, process instances repository, transaction manager, conectors framework, process engine and middleware. In this paper we define and characterize the role and importance of the components of BPMS's framework. The research method adopted was the case study, through the analysis of the implementation of the BPMS solution in an insurance company called Chubb do Brasil. In the case study, the process "Manage Coinsured Events"" is described and characterized, as well as the components of the BPMS solution adopted and implemented by Chubb do Brasil for managing this process.

  6. System for Cooling of Electronic Components

    Science.gov (United States)

    Vasil'ev, L. L.; Grakovich, L. P.; Dragun, L. A.; Zhuravlev, A. S.; Olekhnovich, V. A.; Rabetskii, M. I.

    2017-01-01

    Results of computational and experimental investigations of heat pipes having a predetermined thermal resistance and a system based on these pipes for air cooling of electronic components and diode assemblies of lasers are presented. An efficient compact cooling system comprising heat pipes with an evaporator having a capillary coating of a caked copper powder and a condenser having a developed outer finning, has been deviced. This system makes it possible to remove, to the ambient air, a heat flow of power more than 300 W at a temperature of 40-50°C.

  7. Complement and thrombosis in the antiphospholipid syndrome.

    Science.gov (United States)

    Oku, Kenji; Nakamura, Hiroyuki; Kono, Michihiro; Ohmura, Kazumasa; Kato, Masaru; Bohgaki, Toshiyuki; Horita, Tetsuya; Yasuda, Shinsuke; Amengual, Olga; Atsumi, Tatsuya

    2016-10-01

    The involvement of complement activation in the pathophysiology of antiphospholipid syndrome (APS) was first reported in murine models of antiphospholipid antibody (aPL)-related pregnancy morbidities. We previously reported that complement activation is prevalent and may function as a source of procoagulant cell activation in the sera of APS patients. Recently, autoantibodies against C1q, a component of complement 1, were reported to be correlated with complement activation in systemic lupus erythematosus. These antibodies target neoepitopes of deformed C1q bound to various molecules (i.e., anionic phospholipids) and induce accelerated complement activation. We found that anti-C1q antibodies are more frequently detected in primary APS patients than in control patients and in refractory APS patients with repeated thrombotic events. The titer of anti-C1q antibodies was significantly higher in refractory APS patients than in APS patients without flare. The binding of C1q to anionic phospholipids may be associated with the surge in complement activation in patients with anti-C1q antibodies when triggered by 'second-hit' biological stressors such as infection. Such stressors will induce overexpression of anionic phospholipids, with subsequent increases in deformed C1q that is targeted by anti-C1q antibodies. Copyright © 2016. Published by Elsevier B.V.

  8. FFTF Heat Transport System (HTS) component and system design

    International Nuclear Information System (INIS)

    Young, M.W.; Edwards, P.A.

    1980-01-01

    The FFTF Heat Transport Systems and Components designs have been completed and successfully tested at isothermal conditions up to 427 0 C (800 0 F). General performance has been as predicted in the design analyses. Operational flexibility and reliability have been outstanding throughout the test program. The components and systems have been demonstrated ready to support reactor powered operation testing planned later in 1980

  9. Marketing information system - concept and components

    OpenAIRE

    Domazet Ivana S.

    2003-01-01

    Current conditions for the carrying out of business activities are being characterized by an intensive changing of the business surrounding, as well as by a need for a flexible adapting to the newly-created conditions. Dynamisation of economic ambience, based on the principles of a propulsive marketing economy is affirming a business philosophy which will be based on requests of the strategic marketing. Vital component of every successful company is information system, which helps data acquis...

  10. Shark complement: an assessment.

    Science.gov (United States)

    Smith, S L

    1998-12-01

    The classical (CCP) and alternative (ACP) pathways of complement activation have been established for the nurse shark (Ginglymostoma cirratum). The isolation of a cDNA clone encoding a mannan-binding protein-associated serine protease (MASP)-1-like protein from the Japanese dogfish (Triakis scyllia) suggests the presence of a lectin pathway. The CCP consists of six functionally distinct components: C1n, C2n, C3n, C4n, C8n and C9n, and is activated by immune complexes in the presence of Ca++ and Mg++ ions. The ACP is antibody independent, requiring Mg++ ions and a heat-labile 90 kDa factor B-like protein for activity. Proteins considered homologues of C1q, C3 and C4 (C2n) of the mammalian complement system have been isolated from nurse shark serum. Shark C1q is composed of at least two chain types each showing 50% identity to human C1q chains A and B. Partial sequence of the globular domain of one of the chains shows it to be C1q-like rather than like mannan-binding protein. N-terminal amino acid sequences of the alpha and beta chain of shark C3 and C4 molecules show significant identity with corresponding human C3 and C4 chains. A sequence representing shark C4 gamma chain, shows little similarity to human C4 gamma chain. The terminal shark components C8n and C9n are functional analogues of mammalian C8 and C9. Anaphylatoxin activity has been demonstrated in activated shark serum, and porcine C5a desArg induces shark leucocyte chemotaxis. The deduced amino acid sequence of a partial C3 cDNA clone from the nurse shark shows 50%, 30% and 24% homology with the corresponding region of mammalian C3, C4 and alpha 2-macroglobulin. Deduced amino acid sequence data from partial Bf/C2 cDNA clones, two from the nurse shark and one from the Japanese dogfish, suggest that at least one species of elasmobranch has two distinct Bf/C2 genes.

  11. Toxicity of the main electronic cigarette components, propylene glycol, glycerin, and nicotine, in Sprague-Dawley rats in a 90-day OECD inhalation study complemented by molecular endpoints.

    Science.gov (United States)

    Phillips, Blaine; Titz, Bjoern; Kogel, Ulrike; Sharma, Danilal; Leroy, Patrice; Xiang, Yang; Vuillaume, Grégory; Lebrun, Stefan; Sciuscio, Davide; Ho, Jenny; Nury, Catherine; Guedj, Emmanuel; Elamin, Ashraf; Esposito, Marco; Krishnan, Subash; Schlage, Walter K; Veljkovic, Emilija; Ivanov, Nikolai V; Martin, Florian; Peitsch, Manuel C; Hoeng, Julia; Vanscheeuwijck, Patrick

    2017-11-01

    While the toxicity of the main constituents of electronic cigarette (ECIG) liquids, nicotine, propylene glycol (PG), and vegetable glycerin (VG), has been assessed individually in separate studies, limited data on the inhalation toxicity of them is available when in mixtures. In this 90-day subchronic inhalation study, Sprague-Dawley rats were nose-only exposed to filtered air, nebulized vehicle (saline), or three concentrations of PG/VG mixtures, with and without nicotine. Standard toxicological endpoints were complemented by molecular analyses using transcriptomics, proteomics, and lipidomics. Compared with vehicle exposure, the PG/VG aerosols showed only very limited biological effects with no signs of toxicity. Addition of nicotine to the PG/VG aerosols resulted in effects in line with nicotine effects observed in previous studies, including up-regulation of xenobiotic enzymes (Cyp1a1/Fmo3) in the lung and metabolic effects, such as reduced serum lipid concentrations and expression changes of hepatic metabolic enzymes. No toxicologically relevant effects of PG/VG aerosols (up to 1.520  mg PG/L + 1.890 mg VG/L) were observed, and no adverse effects for PG/VG/nicotine were observed up to 438/544/6.6 mg/kg/day. This study demonstrates how complementary systems toxicology analyses can reveal, even in the absence of observable adverse effects, subtoxic and adaptive responses to pharmacologically active compounds such as nicotine. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  12. Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system

    DEFF Research Database (Denmark)

    Palarasah, Y; Nielsen, C; Sprogøe, U

    2011-01-01

    is therefore of great importance. In this study, we present novel improved enzyme-linked immunosorbent assays for the functional assessment of the three individual pathways of the complement system. The method is applicable at high serum concentrations and we demonstrate that it minimizes both false negative...... as well as false positive results. In particular, for the functional mannose-binding lectin activity it represents an improvement on the existing assays. In this respect, the present assays represent novel improved diagnostic protocols for patients with suspected immunodeficiencies related...

  13. Thermochemical modelling of multi-component systems

    International Nuclear Information System (INIS)

    Sundman, B.; Gueneau, C.

    2015-01-01

    Computational thermodynamic, also known as the Calphad method, is a standard tool in industry for the development of materials and improving processes and there is an intense scientific development of new models and databases. The calculations are based on thermodynamic models of the Gibbs energy for each phase as a function of temperature, pressure and constitution. Model parameters are stored in databases that are developed in an international scientific collaboration. In this way, consistent and reliable data for many properties like heat capacity, chemical potentials, solubilities etc. can be obtained for multi-component systems. A brief introduction to this technique is given here and references to more extensive documentation are provided. (authors)

  14. European Union funded project on the development of a whole complement deficiency screening ELISA

    DEFF Research Database (Denmark)

    Würzner, Reinhard; Tedesco, Francesco; Garred, Peter

    2015-01-01

    A whole complement ELISA-based assay kit, primarily designed to screen for deficiencies in components of the complement system was developed during a European Union grant involving more than a dozen European scientists and a small-medium enterprise company (Wieslab, which later merged into Eurodi......A whole complement ELISA-based assay kit, primarily designed to screen for deficiencies in components of the complement system was developed during a European Union grant involving more than a dozen European scientists and a small-medium enterprise company (Wieslab, which later merged...

  15. Marketing information system - concept and components

    Directory of Open Access Journals (Sweden)

    Domazet Ivana S.

    2003-01-01

    Full Text Available Current conditions for the carrying out of business activities are being characterized by an intensive changing of the business surrounding, as well as by a need for a flexible adapting to the newly-created conditions. Dynamisation of economic ambience, based on the principles of a propulsive marketing economy is affirming a business philosophy which will be based on requests of the strategic marketing. Vital component of every successful company is information system, which helps data acquisition and analysis, and its transfer into information, which is then forwarded to users and management. Bearing that in mind, we have created marketing information system, which collects relevant data, analyses it and then produces information, which can be used in planning, implementation and control, those making your company more efficient.

  16. Thermal Components Boost Performance of HVAC Systems

    Science.gov (United States)

    2012-01-01

    As the International Space Station (ISS) travels 17,500 miles per hour, normal is having a constant sensation of free-falling. Normal is no rain, but an extreme amount of shine.with temperatures reaching 250 F when facing the Sun. Thanks to a number of advanced control systems onboard the ISS, however, the interior of the station remains a cool, comfortable, normal environment where astronauts can live and work for extended periods of time. There are two main control systems on the ISS that make it possible for humans to survive in space: the Thermal Control System (TCS) and the Environmental Control and Life Support system. These intricate assemblies work together to supply water and oxygen, regulate temperature and pressure, maintain air quality, and manage waste. Through artificial means, these systems create a habitable environment for the space station s crew. The TCS constantly works to regulate the temperature not only for astronauts, but for the critical instruments and machines inside the spacecraft as well. To do its job, the TCS encompasses several components and systems both inside and outside of the ISS. Inside the spacecraft, a liquid heat-exchange process mechanically pumps fluids in closed-loop circuits to collect, transport, and reject heat. Outside the ISS, an external system circulates anhydrous ammonia to transport heat and cool equipment, and radiators release the heat into space. Over the years, NASA has worked with a variety of partners.public and private, national and international. to develop and refine the most complex thermal control systems ever built for spacecraft, including the one on the ISS.

  17. Nanomedicine and the complement paradigm.

    Science.gov (United States)

    Moghimi, S Moein; Farhangrazi, Z Shadi

    2013-05-01

    The role of complement in idiosyncratic reactions to nanopharmaceutical infusion is receiving increasing attention. We discuss this in relation to nanopharmaceutical development and the possible use of complement inhibitors to prevent related adverse reactions. We further call on initiation of genetic association studies to unravel the genetic basis of nanomedicine infusion-related adverse responses, since most of the polymorphic genes in the genome belong to the immune system. In this paper, idiosyncratic reactions based on complement activation are discussed in the context of newly available complement inhibitors. Copyright © 2013 Elsevier Inc. All rights reserved.

  18. Micro rapid prototyping system for micro components

    International Nuclear Information System (INIS)

    Li Xiaochun; Choi Hongseok; Yang Yong

    2002-01-01

    Similarities between silicon-based micro-electro-mechanical systems (MEMS) and Shape Deposition Manufacturing (SDM) processes are obvious: both integrate additive and subtractive processes and use part and sacrificial materials to obtain functional structures. These MEMS techniques are two-dimensional (2-D) processes for a limited number of materials while SDM enables the building of parts that have traditionally been impossible to fabricate because of their complex shapes or of their variety in materials. This work presents initial results on the development of a micro rapid prototyping system that adapts SDM methodology to micro-fabrication. This system is designed to incorporate microdeposition and laser micromachining. In the hope of obtaining a precise microdeposition, an ultrasonic-based micro powder-feeding mechanism was developed in order to form thin patterns of dry powders that can be cladded or sintered onto a substrate by a micro-sized laser beam. Furthermore, experimental results on laser micromachining using a laser beam with a wavelength of 355 nm are also presented. After further improvement, the developed micro manufacturing system could take computer-aided design (CAD) output to reproduce 3-D heterogeneous micro-components from a wide selection of materials

  19. Effect of complementation of cattle cooling systems with feedline soakers on lactating dairy cows in a desert environment.

    Science.gov (United States)

    Ortiz, X A; Smith, J F; Bradford, B J; Harner, J P; Oddy, A

    2011-02-01

    Two experiments were conducted on a commercial dairy farm in eastern Saudi Arabia to investigate the effects of Korral Kool (KK; Korral Kool Inc., Mesa, AZ) cattle cooling systems complemented with feedline soakers on core body temperature (CBT) of dairy cows. In both experiments, cows had access to KK 24h/d. In the first experiment, 7 primiparous and 6 multiparous lactating Holstein dairy cows were assigned to 1 of 2 pens, which were assigned randomly to treatment sequence over 4 d in a switchback design. Soakers were on (ON24) or off (OFF24) for 24h/d. For the second experiment, 20 multiparous lactating Holstein cows were assigned randomly to 1 of 2 pens, which were assigned randomly to treatment sequence in a switchback design. This experiment lasted 4 d and feedline soakers alternately remained off or were on (ON12) for 12h/d. In experiment 1, average ambient temperature was 30 ± 0.9°C and average relative humidity was 44 ± 14% (mean ± SD). Feedline soakers complementing KK systems for 24 h/d decreased the mean CBT of lactating dairy cows compared with KK systems alone (38.80 vs. 38.98 ± 0.061°C, respectively). A significant treatment by time interaction was found. The greatest treatment effects occurred at 2100 h; treatment means at this time were 39.26 and 38.85 ± 0.085°C for OFF24 and ON24 treatments, respectively. In experiment 2, average ambient temperature was 35 ± 1.5°C and average relative humidity was 33 ± 16%. Feedline soakers running for 12 h/d significantly decreased the mean 24-h CBT from 39.16 to 38.99 ± 0.084°C. Treatment by time interaction was also significant; the greatest treatment effects occurred at 1500 h, when ON12 reduced CBT from 39.38 to 38.81 ± 0.088°C. These results demonstrate that complementing the KK system with feedline soakers decreased the CBT of dairy cows housed in desert environments. However, the combined systems were not sufficient to lower CBT to normal temperatures in this extreme environment. Copyright

  20. In-vitro effects of tri-iodinated X-ray contrast media on blood coagulation, fibrinolysis and complement system

    International Nuclear Information System (INIS)

    Blanke, D.

    1982-01-01

    In-vitro experiments with Jodipamid, Jothalamat and Diatrizoat served the purpose of determining influences of contrast media on blood coagulation, fibrinolysis and the complement system. For all three contrast media investigated the effect noted was dose-dependent and was only brought about by concentrations higher than physiological ones. Liver-pathway Jodipamid was seen to have a much stronger effect than the two renal-pathway contrast media Jothalamat and Diatrizoat, which is probably due to the different protein binding capacities. In detail, the results with Jodipamid were as follows: a sharp fall in thrombinogen, a distinct decrease in fibrinogen both in the immunological and functional test, but only delayed decrease in complement factor C 4. Fibrinolytic fission products were found after applying the dose of 30 mM, as compared to 400 mM for the renal-pathway contrast media. Furthermore the functional tests (F I and F II) with Jothalamat and Diatrizoat showed only slight effects, the immunological ones (F I and C 4) none at all. The influence of the contrast media on factors I and II is interpreted by the author as an inhibition of fibrin polymerization. What seems to be the verification of fibrinolytic fission products is explained by a non-specific agglutination reaction, the decrease in C 4 by contrast-medium-induced protein denaturation. (orig./MG) [de

  1. Evolution and Function of Thioester-Containing Proteins and the Complement System in the Innate Immune Response

    Directory of Open Access Journals (Sweden)

    Upasana Shokal

    2017-06-01

    Full Text Available The innate immune response is evolutionary conserved among organisms. The complement system forms an important and efficient immune defense mechanism. It consists of plasma proteins that participate in microbial detection, which ultimately results in the production of various molecules with antimicrobial activity. Thioester-containing proteins (TEPs are a superfamily of secreted effector proteins. In vertebrates, certain TEPs act in the innate immune response by promoting recruitment of immune cells, phagocytosis, and direct lysis of microbial invaders. Insects are excellent models for dissecting the molecular basis of innate immune recognition and response to a wide range of microbial infections. Impressive progress in recent years has generated crucial information on the role of TEPs in the antibacterial and antiparasite response of the tractable model insect Drosophila melanogaster and the mosquito malaria vector Anopheles gambiae. This knowledge is critical for better understanding the evolution of TEPs and their involvement in the regulation of the host innate immune system.

  2. Conductivity of two-component systems

    Energy Technology Data Exchange (ETDEWEB)

    Kuijper, A. de; Hofman, J.P.; Waal, J.A. de [Shell Research BV, Rijswijk (Netherlands). Koninklijke/Shell Exploratie en Productie Lab.; Sandor, R.K.J. [Shell International Petroleum Maatschappij, The Hague (Netherlands)

    1996-01-01

    The authors present measurements and computer simulation results on the electrical conductivity of nonconducting grains embedded in a conductive brine host. The shapes of the grains ranged from prolate-ellipsoidal (with an axis ratio of 5:1) through spherical to oblate-ellipsoidal (with an axis ratio of 1:5). The conductivity was studied as a function of porosity and packing, and Archie`s cementation exponent was found to depend on porosity. They used spatially regular and random configurations with aligned and nonaligned packings. The experimental results agree well with the computer simulation data. This data set will enable extensive tests of models for calculating the anisotropic conductivity of two-component systems.

  3. Efficient, trans-complementing packaging systems for chimeric, pseudoinfectious dengue 2/yellow fever viruses

    International Nuclear Information System (INIS)

    Shustov, Alexandr V.; Frolov, Ilya

    2010-01-01

    In our previous studies, we have stated to build a new strategy for developing defective, pseudoinfectious flaviviruses (PIVs) and applying them as a new type of vaccine candidates. PIVs combined the efficiency of live vaccines with the safety of inactivated or subunit vaccines. The results of the present work demonstrate further development of chimeric PIVs encoding dengue virus 2 (DEN2V) glycoproteins and yellow fever virus (YFV)-derived replicative machinery as potential vaccine candidates. The newly designed PIVs have synergistically functioning mutations in the prM and NS2A proteins, which abolish processing of the latter proteins and make the defective viruses capable of producing either only noninfectious, immature and/or subviral DEN2V particles. The PIV genomes can be packaged to high titers into infectious virions in vitro using the NS1-deficient YFV helper RNAs, and both PIVs and helpers can then be passaged as two-component genome viruses at an escalating scale.

  4. Molecular characterization of the alpha subunit of complement component C8 (GcC8alpha) in the nurse shark (Ginglymostoma cirratum).

    Science.gov (United States)

    Aybar, Lydia; Shin, Dong-Ho; Smith, Sylvia L

    2009-09-01

    Target cell lysis by complement is achieved by the assembly and insertion of the membrane attack complex (MAC) composed of glycoproteins C5b through C9. The lytic activity of shark complement involves functional analogues of mammalian C8 and C9. Mammalian C8 is composed of alpha, beta, and gamma subunits. The subunit structure of shark C8 is not known. This report describes a 2341 nucleotide sequence that translates into a polypeptide of 589 amino acid residues, orthologue to mammalian C8alpha and has the same modular architecture with conserved cysteines forming the peptide bond backbone. The C8gamma-binding cysteine is conserved in the perforin-like domain. Hydrophobicity profile indicates the presence of hydrophobic residues essential for membrane insertion. It shares 41.1% and 47.4% identity with human and Xenopus C8alpha respectively. Southern blot analysis showed GcC8alpha exists as a single copy gene expressed in most tissues except the spleen with the liver being the main site of synthesis. Phylogenetic analysis places it in a clade with C8alpha orthologs and as a sister taxa to the Xenopus. 2009 Elsevier Ltd.

  5. PERFORMANCE OF ALTERNATIVE COMPONENT PRICING SYSTEMS FOR PORK

    OpenAIRE

    Brorsen, B. Wade; Akridge, Jay T.; Boland, Michael A.; Mauney, Sean; Forrest, John C.

    1998-01-01

    One method of implementing value-based marketing is a component pricing system. This research develops and evaluates alternative component pricing systems for pork. Two electronic technologies for estimating carcass components (optical probe and electromagnetic scanner) were evaluated on two sets of data representing different populations. Model accuracy increased as additional components were added.

  6. Heterocomplexes of mannose-binding lectin and the pentraxins PTX3 or SAP trigger cross-activation of the complement system

    DEFF Research Database (Denmark)

    Ma, Ying Jie; Doni, Andrea; Skjødt, Mikkel-Ole

    2011-01-01

    The long pentraxin 3 (PTX3), serum amyloid P component (SAP) and C-reactive protein (CRP) belong to the pentraxin family of pattern recognition molecules involved in tissue homeostasis and innate immunity. They interact with C1q from the classical complement pathway. Whether this also occurs via...... the analogous mannose-binding lectin (MBL) from the lectin complement pathway is unknown. Thus, we investigated the possible interaction between MBL and the pentraxins. We report that MBL bound PTX3 and SAP partly via its collagen-like domain, but not CRP. MBL:PTX3 complex formation resulted in recruitment of C......1q, but this was not seen for the MBL:SAP complex. However, both MBL:PTX3 and MBL:SAP complexes enhanced C4 and C3 deposition and opsonophagocytosis of Candida albicans by polymorphonuclear leukocytes. Interaction between MBL and PTX3 lead to communication between the lectin and classical complement...

  7. A flexible continuous-variable QKD system using off-the-shelf components

    Science.gov (United States)

    Comandar, Lucian C.; Brunner, Hans H.; Bettelli, Stefano; Fung, Fred; Karinou, Fotini; Hillerkuss, David; Mikroulis, Spiros; Wang, Dawei; Kuschnerov, Maxim; Xie, Changsong; Poppe, Andreas; Peev, Momtchil

    2017-10-01

    We present the development of a robust and versatile CV-QKD architecture based on commercially available optical and electronic components. The system uses a pilot tone for phase synchronization with a local oscillator, as well as local feedback loops to mitigate frequency and polarization drifts. Transmit and receive-side digital signal processing is performed fully in software, allowing for rapid protocol reconfiguration. The quantum link is complemented with a software stack for secure-key processing, key storage and encrypted communication. All these features allow for the system to be at the same time a prototype for a future commercial product and a research platform.

  8. Peptide Nucleic Acid Knockdown and Intra-host Cell Complementation of Ehrlichia Type IV Secretion System Effector

    Directory of Open Access Journals (Sweden)

    Pratibha Sharma

    2017-06-01

    Full Text Available Survival of Ehrlichia chaffeensis depends on obligatory intracellular infection. One of the barriers to E. chaffeensis research progress has been the inability, using conventional techniques, to generate knock-out mutants for genes essential for intracellular infection. This study examined the use of Peptide Nucleic Acids (PNAs technology to interrupt type IV secretion system (T4SS effector protein expression in E. chaffeensis followed by intracellular complementation of the effector to determine its requirement for infection. Successful E. chaffeensis infection depends on the E. chaffeensis-specific T4SS protein effector, ehrlichial translocated factor-1 (Etf-1, which induces Rab5-regulated autophagy to provide host cytosolic nutrients required for E. chaffeensis proliferation. Etf-1 is also imported by host cell mitochondria where it inhibits host cell apoptosis to prolong its infection. We designed a PNA specific to Etf-1 and showed that the PNA bound to the target region of single-stranded Etf-1 RNA using a competitive binding assay. Electroporation of E. chaffeensis with this PNA significantly reduced Etf-1 mRNA and protein, and the bacteria's ability to induce host cell autophagy and infect host cells. Etf-1 PNA-mediated inhibition of ehrlichial Etf-1 expression and E. chaffeensis infection could be intracellularly trans-complemented by ectopic expression of Etf-1-GFP in host cells. These data affirmed the critical role of bacterial T4SS effector in host cell autophagy and E. chaffeensis infection, and demonstrated the use of PNA to analyze the gene functions of obligate intracellular bacteria.

  9. The Plasmid Complement of Lactococcus lactis UC509.9 Encodes Multiple Bacteriophage Resistance Systems

    Science.gov (United States)

    Ainsworth, Stuart; Mahony, Jennifer

    2014-01-01

    Lactococcus lactis subsp. cremoris strains are used globally for the production of fermented dairy products, particularly hard cheeses. Believed to be of plant origin, L. lactis strains that are used as starter cultures have undergone extensive adaptation to the dairy environment, partially through the acquisition of extrachromosomal DNA in the form of plasmids that specify technologically important phenotypic traits. Here, we present a detailed analysis of the eight plasmids of L. lactis UC509.9, an Irish dairy starter strain. Key industrial phenotypes were mapped, and genes that are typically associated with lactococcal plasmids were identified. Four distinct, plasmid-borne bacteriophage resistance systems were identified, including two abortive infection systems, AbiB and AbiD1, thereby supporting the observed phage resistance of L. lactis UC509.9. AbiB escape mutants were generated for phage sk1, which were found to carry mutations in orf6, which encodes the major capsid protein of this phage. PMID:24814781

  10. The Complement Binding and Inhibitory Protein CbiA of Borrelia miyamotoi Degrades Extracellular Matrix Components by Interacting with Plasmin(ogen

    Directory of Open Access Journals (Sweden)

    Ngoc T. T. Nguyen

    2018-02-01

    Full Text Available The emerging relapsing fever spirochete Borrelia (B. miyamotoi is transmitted by ixodid ticks and causes the so-called hard tick-borne relapsing fever or B. miyamotoi disease (BMD. More recently, we identified a surface-exposed molecule, CbiA exhibiting complement binding and inhibitory capacity and rendering spirochetes resistant to complement-mediated lysis. To gain deeper insight into the molecular principles of B. miyamotoi-host interaction, we examined CbiA as a plasmin(ogen receptor that enables B. miyamotoi to interact with the serine protease plasmin(ogen. Recombinant CbiA was able to bind plasminogen in a dose-dependent fashion. Moreover, lysine residues appear to play a crucial role in the protein-protein interaction as binding of plasminogen was inhibited by the lysine analog tranexamic acid as well as increasing ionic strength. Of relevance, plasminogen bound to CbiA can be converted by urokinase-type plasminogen activator (uPa to active plasmin which cleaved both, the chromogenic substrate S-2251 and its physiologic substrate fibrinogen. Concerning the involvement of specific amino acids in the interaction with plasminogen, lysine residues located at the C-terminus are frequently involved in the binding as reported for various other plasminogen-interacting proteins of Lyme disease spirochetes. Lysine residues located within the C-terminal domain were substituted with alanine to generate single, double, triple, and quadruple point mutants. However, binding of plasminogen to the mutated CbiA proteins was not affected, suggesting that lysine residues distant from the C-terminus might be involved in the interaction.

  11. Effect of the dietary inclusion of soybean components on the innate immune system in zebrafish.

    Science.gov (United States)

    Fuentes-Appelgren, Pamela; Opazo, Rafael; Barros, Luis; Feijoó, Carmen G; Urzúa, Victoria; Romero, Jaime

    2014-02-01

    Some components of plant-based meals, such as saponins and vegetal proteins, have been proposed as inducers of intestinal inflammation in some fish. However, the molecular and cellular bases for this phenomenon have not been reported. In this work, zebrafish were used as a model to evaluate the effects of individual soybean meal components, such as saponins and soy proteins. Zebrafish larvae fed a fish meal feed containing soy components were assessed according to low and high inclusion levels. The granulocytes associated with the digestive tract and the induction of genes related to the immune system were quantitated as markers of the effects of the dietary components. A significant increase in the number of granulocytes was observed after feeding fish diets containing high saponin or soy protein contents. These dietary components also induced the expression of genes related to the innate immune system, including myeloid-specific peroxidase, as well as the complement protein and cytokines. These results reveal the influence of dietary components on the stimulation of the immune system. These observations could be significant to understanding the contributions of saponin and soy protein to the onset of enteritis in aqua-cultured fish, and this knowledge may aid in defining the role of the innate immune system in other inflammatory diseases involving dietary components in mammals.

  12. Reliability for systems of degrading components with distinct component shock sets

    International Nuclear Information System (INIS)

    Song, Sanling; Coit, David W.; Feng, Qianmei

    2014-01-01

    This paper studies reliability for multi-component systems subject to dependent competing risks of degradation wear and random shocks, with distinct shock sets. In practice, many systems are exposed to distinct and different types of shocks that can be categorized according to their sizes, function, affected components, etc. Previous research primarily focuses on simple systems with independent failure processes, systems with independent component time-to-failure, or components that share the same shock set or type of shocks. In our new model, we classify random shocks into different sets based on their sizes or function. Shocks with specific sizes or function can selectively affect one or more components in the system but not necessarily all components. Additionally the shocks from the different shock sets can arrive at different rates and have different relative magnitudes. Preventive maintenance (PM) optimization is conducted for the system with different component shock sets. Decision variables for two different maintenance scheduling problems, the PM replacement time interval, and the PM inspection time interval, are determined by minimizing a defined system cost rate. Sensitivity analysis is performed to provide insight into the behavior of the proposed maintenance policies. These models can be applied directly or customized for many complex systems that experience dependent competing failure processes with different component shock sets. A MEMS (Micro-electro mechanical systems) oscillator is a typical system subject to dependent and competing failure processes, and it is used as a numerical example to illustrate our new reliability and maintenance models

  13. Complement Evasion by Pathogenic Leptospira.

    Science.gov (United States)

    Fraga, Tatiana Rodrigues; Isaac, Lourdes; Barbosa, Angela Silva

    2016-01-01

    Leptospirosis is a neglected infectious disease caused by spirochetes from the genus Leptospira . Pathogenic microorganisms, notably those which reach the blood circulation such as Leptospira , have evolved multiple strategies to escape the host complement system, which is important for innate and acquired immunity. Leptospira avoid complement-mediated killing through: (i) recruitment of host complement regulators; (ii) acquisition of host proteases that cleave complement proteins on the bacterial surface; and, (iii) secretion of proteases that inactivate complement proteins in the Leptospira surroundings. The recruitment of host soluble complement regulatory proteins includes the acquisition of Factor H (FH) and FH-like-1 (alternative pathway), C4b-binding protein (C4BP) (classical and lectin pathways), and vitronectin (Vn) (terminal pathway). Once bound to the leptospiral surface, FH and C4BP retain cofactor activity of Factor I in the cleavage of C3b and C4b, respectively. Vn acquisition by leptospires may result in terminal pathway inhibition by blocking C9 polymerization. The second evasion mechanism lies in plasminogen (PLG) binding to the leptospiral surface. In the presence of host activators, PLG is converted to enzymatically active plasmin, which is able to degrade C3b, C4b, and C5 at the surface of the pathogen. A third strategy used by leptospires to escape from complement system is the active secretion of proteases. Pathogenic, but not saprophytic leptospires, are able to secrete metalloproteases that cleave C3 (central complement molecule), Factor B (alternative pathway), and C4 and C2 (classical and lectin pathways). The purpose of this review is to fully explore these complement evasion mechanisms, which act together to favor Leptospira survival and multiplication in the host.

  14. Applications of Advanced Electromagnetics Components and Systems

    CERN Document Server

    Kouzaev, Guennadi A

    2013-01-01

    This text, directed to the microwave engineers and Master and PhD students, is on the use of electromagnetics to the development and design of advanced integrated components distinguished by their extended field of applications. The results of hundreds of authors scattered in numerous journals and conference proceedings are carefully reviewed and classed.  Several chapters are to refresh the knowledge of readers in advanced electromagnetics. New techniques are represented by compact electromagnetic–quantum equations which can be used in modeling of microwave-quantum integrated circuits of future In addition, a topological method to the boundary value problem analysis is considered with the results and examples.  One extended chapter is for the development and design of integrated components for extended bandwidth applications, and the technology and electromagnetic issues of silicon integrated transmission lines, transitions, filters, power dividers, directional couplers, etc are considered. Novel prospec...

  15. Requirements for containment system components in CANDU nuclear power plants

    International Nuclear Information System (INIS)

    1988-02-01

    This Standard specifies the requirements and establishes the rules for design, fabrication, and installation of pressure-retaining containment system components. In this Standard the term 'components' includes non registered items

  16. Requirements for containment system components in CANDU nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1988-02-01

    This Standard specifies the requirements and establishes the rules for design, fabrication, and installation of pressure-retaining containment system components. In this Standard the term `components` includes non registered items.

  17. Components of Maternal Healthcare Delivery System Contributing to ...

    African Journals Online (AJOL)

    Components of Maternal Healthcare Delivery System Contributing to Maternal Deaths ... transcripts were analyzed using a directed approach to content analysis. Excerpts were categorized according to three main components of the maternal ...

  18. Subversion of complement by hematophagous parasites

    OpenAIRE

    Schroeder, Hélène; Skelly, Patrick; Zipfel, Peter F.; Losson, Bertrand; Vanderplasschen, Alain

    2009-01-01

    The complement system is a crucial part of innate and adaptive immunity which exerts a significant evolutionary pressure on pathogens. It has selected for those pathogens, mainly micro-organisms but also parasites, that have evolved countermeasures. The characterization of how pathogens evade complement attack is a rapidly developing field of current research. In recent years, multiple complement evasion strategies have been characterized. In this review, we focus on complement escape mechani...

  19. Gasoline engine management systems and components

    CERN Document Server

    2015-01-01

    The call for environmentally compatible and economical vehicles necessitates immense efforts to develop innovative engine concepts. Technical concepts such as gasoline direct injection helped to save fuel up to 20 % and reduce CO2-emissions. Descriptions of the cylinder-charge control, fuel injection, ignition and catalytic emission-control systems provides comprehensive overview of today´s gasoline engines. This book also describes emission-control systems and explains the diagnostic systems. The publication provides information on engine-management-systems and emission-control regulations. Contents History of the automobile.- Basics of the gasoline engine.- Fuels.- Cylinder-charge control systems.- Gasoline injection systems over the years.- Fuel supply.- Manifold fuel injection.- Gasoline direct injection.- Operation of gasoline engines on natural gas.- Ignition systems over the years.- Inductive ignition systems.- Ignition coils.- Spark plugs.- Electronic control.- Sensors.- Electronic control unit.- Exh...

  20. Application of Component Technology to E-commerce System

    Institute of Scientific and Technical Information of China (English)

    ZHU Jianfeng

    2004-01-01

    At present E-commerce system tends to become more complex, and traditional system designing methods can not fufil the need of E-commerce system, thus requiring an effective methodas solution. With this concern, this paper introduces some concepts of component technology, then brings forward the new connotation and basic features of component technology through the analysis of its technological character. This paper finally discusses the application of component technology to E-commerce system.

  1. CSF coccidioides complement fixation

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/003526.htm CSF coccidioides complement fixation test To use the sharing features on this page, please enable JavaScript. CSF coccidioides complement fixation is a test that checks ...

  2. The development of component-based information systems

    CERN Document Server

    Cesare, Sergio de; Macredie, Robert

    2015-01-01

    This work provides a comprehensive overview of research and practical issues relating to component-based development information systems (CBIS). Spanning the organizational, developmental, and technical aspects of the subject, the original research included here provides fresh insights into successful CBIS technology and application. Part I covers component-based development methodologies and system architectures. Part II analyzes different aspects of managing component-based development. Part III investigates component-based development versus commercial off-the-shelf products (COTS), includi

  3. Studies on coagulation, fibrinolysis, kallikrein-kinin and complement activation in systemic and pulmonary circulation during hip arthroplasty with acrylic cement.

    Science.gov (United States)

    Dahl, O E; Molnar, I; Vinje, A; Rø, J S; Kierulf, P; Andersen, A B; Dalaker, K; Prydz, H

    1988-06-15

    This study was designed to evaluate the contribution of the plasma cascade systems to the cardiopulmonary complications, occasionally leading to sudden death during hip arthroplasty using acrylic cement. The intraoperative pattern following uneventful surgery was therefore investigated in 8 patients with osteoarthrosis with frequent sampling from the radial and pulmonary arteries. The following general findings emerged: A gradual consumption of coagulation factors (platelets, fibrinogen, factor VII, antithrombin III), fibrinolytic components (plasminogen, alpha-2-antiplasmin), kallikrein-kinin factors (prekallikrein, kallikrein inhibitor) and complement factors (C3c, C4) was observed. Some intrapulmonary proteolytic inhibition was noticed as evidenced by lower arterial than mixed venous blood levels of alpha-2-antiplasmin and kallikrein inhibitor. Insignificant changes occurred for factor VII-phospholipid complex. A rapid, massive and transient increase in fibrinopeptide A (FPA) values in the radial artery, as opposed to the moderate increase in the pulmonary artery, was found immediately after reaming and broaching of bone. This probably reflected intrapulmonary fibrinogen to fibrin conversion, reaching a maximum 15-20 minutes before the femoral implantation. As estimated from the FPA arterial peak values and the fall in fibrinogen concentrations, approximately 5-10% of the circulating fibrinogen molecules were devoided of FPA during this intraoperative phase. The marked a-v difference in FPA level supports earlier findings of intrapulmonary fibrin formation and deposition. This process, however preceded the critical period of cardiorespiratory collapse (CRC) by at least 15 minutes, and may thus predispose to this complication.

  4. Deciphering complement mechanisms: The contributions of structural biology.

    NARCIS (Netherlands)

    Arlaud, G.J.; Barlow, P.N.; Gaboriaud, C.; Gros, P.; Narayana, S.V.L.

    2007-01-01

    Since the resolution of the first three-dimensional structure of a complement component in 1980, considerable efforts have been put into the investigation of this system through structural biology techniques, resulting in about a hundred structures deposited in the Protein Data Bank by the beginning

  5. Power system protection 1 principles and components

    CERN Document Server

    Association, Electricity Training

    1995-01-01

    The worldwide growth in demand for electricity has forced the pace of developments in electrical power system design to meet consumer needs for reliable, secure and cheap supplies. Power system protection, as a technology essential to high quality supply, is widely recognised as a specialism of growing and often critical importance, in which power system needs and technological progress have combined to result in rapid developments in policy and practice in recent years. In the United Kingdom, the need for appropriate training in power system protection was recognised in the early 1960s with t

  6. DIGITAL SUBSTATION COMPONENT SYSTEM "SMART GRID"

    Directory of Open Access Journals (Sweden)

    V.I. Vasilchenko

    2014-12-01

    Full Text Available New production technologies of modern control systems have moved from the stage of research and experimentation into the stage of practical use. Modern communication standards for the exchange of information are developed and introduced. Digital devices, protectors and automation are widely used. There has been substantial development of hardware and software of control systems.

  7. Novel Devices and Components for THz Systems

    Science.gov (United States)

    2014-04-25

    effectively . The cage rod system is again 30 mm...often used to reflect RF radiation, or create Faraday cages , this concept can be applied to the FPI mirrors. Wire meshes will simulate solid conductors...Single peice construction should resist leaning more effectively . The cage rod system is again 30 mm. 175 wire-mesh-mirrors. Fixing the rocking

  8. Reactor component inventory system at FFTF

    International Nuclear Information System (INIS)

    Ordonez, C.R.; Redekopp, R.D.; Reed, E.A.

    1985-02-01

    A reliable inventory control system was developed at the Fast Flux Test Facility (FFTF) to keep track of the occupancy of 900 refueling facility locations, to compile historical data on the movement of each reactor assembly, and to simulate assembly moves. The simulate capability is valuable because it allows verification of documents before they are issued for use in the plant, and eliminates the possibility of planning illegal or impossible moves. The system is installed on a UNIVAC 1100 computer and is maintained using a data base management system by Sperry Univac called MAPPER

  9. Expert system for accelerator single-freedom nonlinear components

    International Nuclear Information System (INIS)

    Wang Sheng; Xie Xi; Liu Chunliang

    1995-01-01

    An expert system by Arity Prolog is developed for accelerator single-freedom nonlinear components. It automatically yields any order approximate analytical solutions for various accelerator single-freedom nonlinear components. As an example, the eighth order approximate analytical solution is derived by this expert system for a general accelerator single-freedom nonlinear component, showing that the design of the expert system is successful

  10. Artificial heart system thermal insulation component development

    International Nuclear Information System (INIS)

    Svedberg, R.C.; Buckman, R.W. Jr.

    1975-01-01

    A concentric cup vacuum multifoil insulation system has been selected by virtue of its size, weight, and thermal performance to insulate the hot radioisotope portion of the thermal converter of an artificial implantable heart system. A factor of 2 improvement in thermal performance, based on the heat loss per number of foil layers (minimum system weight and volume) has been realized over conventional spiral wrapped multifoil vacuum insulation. This improvement is the result of the concentric cup construction to maintain a uniform interfoil spacing and the elimination of corner heat losses. Based on external insulation system dimensions (surface area in contact with host body), heat losses of 0.019 W/ cm 2 at 1140 0 K (1600 0 F) and 0.006 W/cm 2 at 920 0 K (1200 0 F) have been achieved. Factors which influence thermal performance of the nickel foil concentric cup insulation system include the number of cups, configuration and method of application of zirconia (ZrO 2 ) spacer material, system pressure, emittance of the cups, and operating temperature

  11. Catalog of components for electric and hybrid vehicle propulsion systems

    Science.gov (United States)

    Eissler, H. C.

    1981-01-01

    This catalog of commercially available electric and hybrid vehicle propulsion system components is intended for designers and builders of these vehicles and contains 50 categories of components. These categories include those components used between the battery terminals and the output axle hub, as well as some auxiliary equipment. An index of the components and a listing of the suppliers and their addresses and phone numbers are included.

  12. Imprecise system reliability and component importance based on survival signature

    International Nuclear Information System (INIS)

    Feng, Geng; Patelli, Edoardo; Beer, Michael; Coolen, Frank P.A.

    2016-01-01

    The concept of the survival signature has recently attracted increasing attention for performing reliability analysis on systems with multiple types of components. It opens a new pathway for a structured approach with high computational efficiency based on a complete probabilistic description of the system. In practical applications, however, some of the parameters of the system might not be defined completely due to limited data, which implies the need to take imprecisions of component specifications into account. This paper presents a methodology to include explicitly the imprecision, which leads to upper and lower bounds of the survival function of the system. In addition, the approach introduces novel and efficient component importance measures. By implementing relative importance index of each component without or with imprecision, the most critical component in the system can be identified depending on the service time of the system. Simulation method based on survival signature is introduced to deal with imprecision within components, which is precise and efficient. Numerical example is presented to show the applicability of the approach for systems. - Highlights: • Survival signature is a novel way for system reliability and component importance • High computational efficiency based on a complete description of system. • Include explicitly the imprecision, which leads to bounds of the survival function. • A novel relative importance index is proposed as importance measure. • Allows to identify critical components depending on the service time of the system.

  13. Complement elevation in spinal cord injury.

    Science.gov (United States)

    Rebhun, J; Botvin, J

    1980-05-01

    Laboratory studies revealed an elevated complement in 66% of patients with spinal cord injury. It is postulated that the activated complement may be a component of self-feeding immunological mechanism responsible for the failure of regeneration of a mature mammalian spinal cord. There was no evidence that such an injury had any effect on pre-existing atopy.

  14. The complement system in age-related macular degeneration: A review of rare genetic variants and implications for personalized treatment

    NARCIS (Netherlands)

    Geerlings, M.J.; Jong, E.K.; Hollander, A.I. den

    2017-01-01

    Age-related macular degeneration (AMD) is a progressive retinal disease and the major cause of irreversible vision loss in the elderly. Numerous studies have found both common and rare genetic variants in the complement pathway to play a role in the pathogenesis of AMD. In this review we provide an

  15. Advanced Reconnaissance System Component Reliability Study

    Science.gov (United States)

    1956-07-31

    dielectrics. Gaseous dielectrics such as sulphur hexafluoride and ’ fluorocarbons at two to three atmospheres. Fluorinated liquid dielectrics. 3) The...limits. (2) determine compatibility with varnish treatments, (3) compatibility in a complete insulation system. Mechanical and thermal limits of...of a varnish to have good • adhersion, provide an element of flexibility and be chemically compatible with’the wire it is impregnating.. Factors of

  16. Algorithmic fault tree construction by component-based system modeling

    International Nuclear Information System (INIS)

    Majdara, Aref; Wakabayashi, Toshio

    2008-01-01

    Computer-aided fault tree generation can be easier, faster and less vulnerable to errors than the conventional manual fault tree construction. In this paper, a new approach for algorithmic fault tree generation is presented. The method mainly consists of a component-based system modeling procedure an a trace-back algorithm for fault tree synthesis. Components, as the building blocks of systems, are modeled using function tables and state transition tables. The proposed method can be used for a wide range of systems with various kinds of components, if an inclusive component database is developed. (author)

  17. System Risk Balancing Profiles: Software Component

    Science.gov (United States)

    Kelly, John C.; Sigal, Burton C.; Gindorf, Tom

    2000-01-01

    The Software QA / V&V guide will be reviewed and updated based on feedback from NASA organizations and others with a vested interest in this area. Hardware, EEE Parts, Reliability, and Systems Safety are a sample of the future guides that will be developed. Cost Estimates, Lessons Learned, Probability of Failure and PACTS (Prevention, Avoidance, Control or Test) are needed to provide a more complete risk management strategy. This approach to risk management is designed to help balance the resources and program content for risk reduction for NASA's changing environment.

  18. Multilayer electronic component systems and methods of manufacture

    Science.gov (United States)

    Thompson, Dane (Inventor); Wang, Guoan (Inventor); Kingsley, Nickolas D. (Inventor); Papapolymerou, Ioannis (Inventor); Tentzeris, Emmanouil M. (Inventor); Bairavasubramanian, Ramanan (Inventor); DeJean, Gerald (Inventor); Li, RongLin (Inventor)

    2010-01-01

    Multilayer electronic component systems and methods of manufacture are provided. In this regard, an exemplary system comprises a first layer of liquid crystal polymer (LCP), first electronic components supported by the first layer, and a second layer of LCP. The first layer is attached to the second layer by thermal bonds. Additionally, at least a portion of the first electronic components are located between the first layer and the second layer.

  19. Component-Based Approach in Learning Management System Development

    Science.gov (United States)

    Zaitseva, Larisa; Bule, Jekaterina; Makarov, Sergey

    2013-01-01

    The paper describes component-based approach (CBA) for learning management system development. Learning object as components of e-learning courses and their metadata is considered. The architecture of learning management system based on CBA being developed in Riga Technical University, namely its architecture, elements and possibilities are…

  20. Feature-based component model for design of embedded systems

    Science.gov (United States)

    Zha, Xuan Fang; Sriram, Ram D.

    2004-11-01

    An embedded system is a hybrid of hardware and software, which combines software's flexibility and hardware real-time performance. Embedded systems can be considered as assemblies of hardware and software components. An Open Embedded System Model (OESM) is currently being developed at NIST to provide a standard representation and exchange protocol for embedded systems and system-level design, simulation, and testing information. This paper proposes an approach to representing an embedded system feature-based model in OESM, i.e., Open Embedded System Feature Model (OESFM), addressing models of embedded system artifacts, embedded system components, embedded system features, and embedded system configuration/assembly. The approach provides an object-oriented UML (Unified Modeling Language) representation for the embedded system feature model and defines an extension to the NIST Core Product Model. The model provides a feature-based component framework allowing the designer to develop a virtual embedded system prototype through assembling virtual components. The framework not only provides a formal precise model of the embedded system prototype but also offers the possibility of designing variation of prototypes whose members are derived by changing certain virtual components with different features. A case study example is discussed to illustrate the embedded system model.

  1. A Metalloproteinase Mirolysin of Tannerella forsythia Inhibits All Pathways of the Complement System

    DEFF Research Database (Denmark)

    Jusko, Monika; Potempa, Jan; Mizgalska, Danuta

    2015-01-01

    Recent reports focusing on virulence factors of periodontal pathogens implicated proteinases as major determinants of remarkable pathogenicity of these species, with special emphasis on their capacity to modulate complement activity. In particular, bacteria-mediated cleavage of C5 and subsequent...... release of C5a seems to be an important phenomenon in the manipulation of the local inflammatory response in periodontitis. In this study, we present mirolysin, a novel metalloproteinase secreted by Tannerella forsythia, a well-recognized pathogen strongly associated with periodontitis. Mirolysin...... with karilysin, as well as a cysteine proteinase of another periodontal pathogen, Prevotella intermedia, resulted in a strong synergistic effect on complement. Furthermore, mutant strains of T. forsythia, devoid of either mirolysin or karilysin, showed diminished survival in human serum, providing further...

  2. Spores of Mucor ramosissimus, Mucor plumbeus and Mucor circinelloides and their ability to activate human complement system in vitro.

    Science.gov (United States)

    Granja, Luiz Fernando Zmetek; Pinto, Lysianne; Almeida, Cátia Amancio; Alviano, Daniela Sales; Da Silva, Maria Helena; Ejzemberg, Regina; Alviano, Celuta Sales

    2010-03-01

    Complement activation by spores of Mucor ramosissimus, Mucor plumbeus and Mucor circinelloides was studied using absorbed human serum in the presence or absence of chelators (EGTA or EDTA). We found that the spore caused full complement activation when incubated with EGTA-Mg2+ or without chelators, indicating that the alternative pathway is mainly responsible for this response. In order to compare activation profiles from each species, ELISAs for C3 and C4 fragments, mannan binding lectin (MBL), C-reactive protein (CRP) and IgG studies were carried out. All proteins were present on the species tested. Immunofluorescence tests demonstrated the presence of C3 fragments on the surface of all samples, which were confluent throughout fungal surfaces. The same profile of C3, C4, MBL, CRP and IgG deposition, observed in all species, suggests a similar activation behavior for these species.

  3. Ranking of risk significant components for the Davis-Besse Component Cooling Water System

    International Nuclear Information System (INIS)

    Seniuk, P.J.

    1994-01-01

    Utilities that run nuclear power plants are responsible for testing pumps and valves, as specified by the American Society of Mechanical Engineers (ASME) that are required for safe shutdown, mitigating the consequences of an accident, and maintaining the plant in a safe condition. These inservice components are tested according to ASME Codes, either the earlier requirements of the ASME Boiler and Pressure Vessel Code, Section XI, or the more recent requirements of the ASME Operation and Maintenance Code, Section IST. These codes dictate test techniques and frequencies regardless of the component failure rate or significance of failure consequences. A probabilistic risk assessment or probabilistic safety assessment may be used to evaluate the component importance for inservice test (IST) risk ranking, which is a combination of failure rate and failure consequences. Resources for component testing during the normal quarterly verification test or postmaintenance test are expensive. Normal quarterly testing may cause component unavailability. Outage testing may increase outage cost with no real benefit. This paper identifies the importance ranking of risk significant components in the Davis-Besse component cooling water system. Identifying the ranking of these risk significant IST components adds technical insight for developing the appropriate test technique and test frequency

  4. Reincarnation of ancient links between coagulation and complement.

    Science.gov (United States)

    Conway, E M

    2015-06-01

    Throughout evolution, organisms have developed means to contain wounds by simultaneously limiting bleeding and eliminating pathogens and damaged host cells via the recruitment of innate defense mechanisms. Disease emerges when there is unchecked activation of innate immune and/or coagulation responses. A key component of innate immunity is the complement system. Concurrent excess activation of coagulation and complement - two major blood-borne proteolytic pathways - is evident in numerous diseases, including atherosclerosis, diabetes, venous thromboembolic disease, thrombotic microangiopathies, arthritis, cancer, and infectious diseases. Delineating the cross-talk between these two cascades will uncover novel therapeutic insights. © 2015 International Society on Thrombosis and Haemostasis.

  5. A metalloproteinase karilysin present in the majority of Tannerella forsythia isolates inhibits all pathways of the complement system

    DEFF Research Database (Denmark)

    Jusko, Monika; Potempa, Jan; Karim, Abdulkarim Y

    2012-01-01

    Tannerella forsythia is a poorly studied pathogen despite being one of the main causes of periodontitis, which is an inflammatory disease of the supporting structures of the teeth. We found that despite being recognized by all complement pathways, T. forsythia is resistant to killing by human....... forsythia obtained from patients with periodontitis. Taken together, the newly characterized karilysin appears to be an important virulence factor of T. forsythia and might have several important implications for immune evasion....

  6. Activation of the human complement system by cholesterol-rich and pegylated liposomes - Modulation of cholesterol-rich liposome-mediated complement activation by elevated serum LDL and HDL levels

    DEFF Research Database (Denmark)

    Moghimi, S.M.; Hamad, I.; Bunger, R.

    2006-01-01

    level of S-protein-bound form of the terminal complex (SC5b-9). However, liposome-induced rise of SC5b-9 was significantly suppressed when serum HDL cholesterol levels increased by 30%. Increase of serum LDL to levels similar to that observed in heterozygous familial hypercholesterolemia also suppressed......Intravenously infused liposomes may induce cardiopulmonary distress in some human subjects, which is a manifestation of "complement activation-related pseudoallergy." We have now examined liposome-mediated complement activation in human sera with elevated lipoprotein (LDL and HDL) levels, since...... abnormal or racial differences in serum lipid profiles seem to modulate the extent of complement activation and associated adverse responses. In accordance with our earlier observations, cholesterol-rich (45 mol% cholesterol) liposomes activated human complement, as reflected by a significant rise in serum...

  7. Multi-level predictive maintenance for multi-component systems

    International Nuclear Information System (INIS)

    Nguyen, Kim-Anh; Do, Phuc; Grall, Antoine

    2015-01-01

    In this paper, a novel predictive maintenance policy with multi-level decision-making is proposed for multi-component system with complex structure. The main idea is to propose a decision-making process considered on two levels: system level and component one. The goal of the decision rules at the system level is to address if preventive maintenance actions are needed regarding the predictive reliability of the system. At component level the decision rules aim at identifying optimally a group of several components to be preventively maintained when preventive maintenance is trigged due to the system level decision. Selecting optimal components is based on a cost-based group improvement factor taking into account the predictive reliability of the components, the economic dependencies as well as the location of the components in the system. Moreover, a cost model is developed to find the optimal maintenance decision variables. A 14-component system is finally introduced to illustrate the use and the performance of the proposed predictive maintenance policy. Different sensitivity analysis are also investigated and discussed. Indeed, the proposed policy provides more flexibility in maintenance decision-making for complex structure systems, hence leading to significant profits in terms of maintenance cost when compared with existing policies. - Highlights: • A predictive maintenance policy for complex structure systems is proposed. • Multi-level decision process based on prognostic results is proposed. • A cost-based group importance measure is introduced for decision-making. • Both positive and negative dependencies between components are investigated. • A cost model and Monte Carlo simulation are developed for optimization process.

  8. The Location GNSS Modules for the Components of Proteus System

    Science.gov (United States)

    Brzostowski, K.; Darakchiev, R.; Foks-Ryznar, A.; Sitek, P.

    2012-01-01

    The Proteus system - the Integrated Mobile System for Counterterrorism and Rescue Operations is a complex innovative project. To assure the best possible localization of mobile components of the system, many different Global Navigation Satellite System (GNSS) modules were taken into account. In order to chose the best solution many types of tests were done. Full results and conclusions are presented in this paper. The idea of measurements was to test modules in GPS Standard Positioning Service (SPS) with EGNOS system specification according to certain algorithms. The tests had to answer the question: what type of GNSS modules should be used on different components with respect to specific usage of Proteus system. The second goal of tests was to check the solution quality of integrated GNSS/INS (Inertial Navigation System) and its possible usage in some Proteus system components.

  9. Safety classification of nuclear power plant systems, structures and components

    International Nuclear Information System (INIS)

    1992-01-01

    The Safety Classification principles used for the systems, structures and components of a nuclear power plant are detailed in the guide. For classification, the nuclear power plant is divided into structural and operational units called systems. Every structure and component under control is included into some system. The Safety Classes are 1, 2 and 3 and the Class EYT (non-nuclear). Instructions how to assign each system, structure and component to an appropriate safety class are given in the guide. The guide applies to new nuclear power plants and to the safety classification of systems, structures and components designed for the refitting of old nuclear power plants. The classification principles and procedures applying to the classification document are also given

  10. SIGN-R1 and complement factors are involved in the systemic clearance of radiation-induced apoptotic cells in whole-body irradiated mice

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jin-Yeon; Loh, SoHee; Cho, Eun-hee [Department of Biomedical Science & Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701 (Korea, Republic of); Choi, Hyeong-Jwa [Division of Radiation Effect, Korea Institute of Radiological and Medical Sciences, 215-4, 75 Nowon gil Nowon-Gu, Seoul, 139-706 (Korea, Republic of); Na, Tae-Young [College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-741 (Korea, Republic of); Nemeno, Judee Grace E.; Lee, Jeong Ik [Regenerative Medicine Laboratory, Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 143-701 (Korea, Republic of); Yoon, Taek Joon [Department of Food and Nutrition, Yuhan College, Bucheon, Gyeonggi-do, 422-749 (Korea, Republic of); Choi, In-Soo [Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701 (Korea, Republic of); Lee, Minyoung [Division of Radiation Effect, Korea Institute of Radiological and Medical Sciences, 215-4, 75 Nowon gil Nowon-Gu, Seoul, 139-706 (Korea, Republic of); Lee, Jae-Seon [Department of Biomedical Sciences, College of Medicine, Inha University, Incheon, 400-712 (Korea, Republic of); Kang, Young-Sun, E-mail: kangys1967@naver.com [Department of Biomedical Science & Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701 (Korea, Republic of); Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701 (Korea, Republic of)

    2015-08-07

    Although SIGN-R1-mediated complement activation pathway has been shown to enhance the systemic clearance of apoptotic cells, the role of SIGN-R1 in the clearance of radiation-induced apoptotic cells has not been characterized and was investigated in this study. Our data indicated that whole-body γ-irradiation of mice increased caspase-3{sup +} apoptotic lymphocyte numbers in secondary lymphoid organs. Following γ-irradiation, SIGN-R1 and complements (C4 and C3) were simultaneously increased only in the mice spleen tissue among the assessed tissues. In particular, C3 was exclusively activated in the spleen. The delayed clearance of apoptotic cells was markedly prevalent in the spleen and liver of SIGN-R1 KO mice, followed by a significant increase of CD11b{sup +} cells. These results indicate that SIGN-R1 and complement factors play an important role in the systemic clearance of radiation-induced apoptotic innate immune cells to maintain tissue homeostasis after γ-irradiation. - Highlights: • Splenic SIGN-R1{sup +} macrophages are activated after γ-irradiation. • C3 and C4 levels increased and C3 was activated in the spleen after γ-irradiation. • SIGN-R1 mediated the systemic clearance of radiation-induced apoptotic cells in spleen and liver.

  11. SIGN-R1 and complement factors are involved in the systemic clearance of radiation-induced apoptotic cells in whole-body irradiated mice

    International Nuclear Information System (INIS)

    Park, Jin-Yeon; Loh, SoHee; Cho, Eun-hee; Choi, Hyeong-Jwa; Na, Tae-Young; Nemeno, Judee Grace E.; Lee, Jeong Ik; Yoon, Taek Joon; Choi, In-Soo; Lee, Minyoung; Lee, Jae-Seon; Kang, Young-Sun

    2015-01-01

    Although SIGN-R1-mediated complement activation pathway has been shown to enhance the systemic clearance of apoptotic cells, the role of SIGN-R1 in the clearance of radiation-induced apoptotic cells has not been characterized and was investigated in this study. Our data indicated that whole-body γ-irradiation of mice increased caspase-3 + apoptotic lymphocyte numbers in secondary lymphoid organs. Following γ-irradiation, SIGN-R1 and complements (C4 and C3) were simultaneously increased only in the mice spleen tissue among the assessed tissues. In particular, C3 was exclusively activated in the spleen. The delayed clearance of apoptotic cells was markedly prevalent in the spleen and liver of SIGN-R1 KO mice, followed by a significant increase of CD11b + cells. These results indicate that SIGN-R1 and complement factors play an important role in the systemic clearance of radiation-induced apoptotic innate immune cells to maintain tissue homeostasis after γ-irradiation. - Highlights: • Splenic SIGN-R1 + macrophages are activated after γ-irradiation. • C3 and C4 levels increased and C3 was activated in the spleen after γ-irradiation. • SIGN-R1 mediated the systemic clearance of radiation-induced apoptotic cells in spleen and liver

  12. Complement in the Initiation and Evolution of Rheumatoid Arthritis

    Science.gov (United States)

    Holers, V. Michael; Banda, Nirmal K.

    2018-01-01

    The complement system is a major component of the immune system and plays a central role in many protective immune processes, including circulating immune complex processing and clearance, recognition of foreign antigens, modulation of humoral and cellular immunity, removal of apoptotic and dead cells, and engagement of injury resolving and tissue regeneration processes. In stark contrast to these beneficial roles, however, inadequately controlled complement activation underlies the pathogenesis of human inflammatory and autoimmune diseases, including rheumatoid arthritis (RA) where the cartilage, bone, and synovium are targeted. Recent studies of this disease have demonstrated that the autoimmune response evolves over time in an asymptomatic preclinical phase that is associated with mucosal inflammation. Notably, experimental models of this disease have demonstrated that each of the three major complement activation pathways plays an important role in recognition of injured joint tissue, although the lectin and amplification pathways exhibit particularly impactful roles in the initiation and amplification of damage. Herein, we review the complement system and focus on its multi-factorial role in human patients with RA and experimental murine models. This understanding will be important to the successful integration of the emerging complement therapeutics pipeline into clinical care for patients with RA. PMID:29892280

  13. Availability, reliability and downtime of systems with repairable components

    DEFF Research Database (Denmark)

    Kiureghian, Armen Der; Ditlevsen, Ove Dalager; Song, J.

    2007-01-01

    Closed-form expressions are derived for the steady-state availability, mean rate of failure, mean duration of downtime and lower bound reliability of a general system with randomly and independently failing repairable components. Component failures are assumed to be homogeneous Poisson events in ...

  14. Complement component 3: characterization and association with ...

    Indian Academy of Sciences (India)

    NERMIN EL-HALAWANY

    encodes a putative protein of 1661 amino acids—as in cattle—and includes all the functional domains. ... The association analysis of the detected SNPs with milk somatic cell score as an ...... lative milk, fat and protein yields in first lactation.

  15. Genetics Home Reference: complement component 8 deficiency

    Science.gov (United States)

    ... in people with Hispanic, Japanese, or African Caribbean heritage, whereas type II primarily occurs in people of Northern European descent. Related Information What information about a genetic condition can statistics provide? Why are some genetic ...

  16. A hybrid two-component system protein from Azospirillum brasilense Sp7 was involved in chemotaxis.

    Science.gov (United States)

    Cui, Yanhua; Tu, Ran; Wu, Lixian; Hong, Yuanyuan; Chen, Sanfeng

    2011-09-20

    We here report the sequence and functional analysis of org35 of Azospirillum brasilense Sp7, which was originally identified to be able to interact with NifA in yeast-two-hybrid system. The org35 encodes a hybrid two-component system protein, including N-terminal PAS domains, a histidine kinase (HPK) domain and a response regulator (RR) domain in C-terminal. To determine the function of the Org35, a deletion-insertion mutant in PAS domain [named Sp7353] and a complemental strain Sp7353C were constructed. The mutant had reduced chemotaxis ability compared to that of wild-type, and the complemental strain was similar to the wild-type strain. These data suggested that the A. brasilense org35 played a key role in chemotaxis. Variants containing different domains of the org35 were expressed, and the functions of these domains were studied in vitro. Phosphorylation assays in vitro demonstrated that the HPK domain of Org35 possessed the autokinase activity and that the phosphorylated HPK was able to transfer phosphate groups to the RR domain. The result indicated Org35 was a phosphorylation-communicating protein. Copyright © 2010 Elsevier GmbH. All rights reserved.

  17. Verifying Embedded Systems using Component-based Runtime Observers

    DEFF Research Database (Denmark)

    Guan, Wei; Marian, Nicolae; Angelov, Christo K.

    against formally specified properties. This paper presents a component-based design method for runtime observers, which are configured from instances of prefabricated reusable components---Predicate Evaluator (PE) and Temporal Evaluator (TE). The PE computes atomic propositions for the TE; the latter...... is a reconfigurable component processing a data structure, representing the state transition diagram of a non-deterministic state machine, i.e. a Buchi automaton derived from a system property specified in Linear Temporal Logic (LTL). Observer components have been implemented using design models and design patterns...

  18. Defense Strategies for Asymmetric Networked Systems with Discrete Components

    Directory of Open Access Journals (Sweden)

    Nageswara S. V. Rao

    2018-05-01

    Full Text Available We consider infrastructures consisting of a network of systems, each composed of discrete components. The network provides the vital connectivity between the systems and hence plays a critical, asymmetric role in the infrastructure operations. The individual components of the systems can be attacked by cyber and physical means and can be appropriately reinforced to withstand these attacks. We formulate the problem of ensuring the infrastructure performance as a game between an attacker and a provider, who choose the numbers of the components of the systems and network to attack and reinforce, respectively. The costs and benefits of attacks and reinforcements are characterized using the sum-form, product-form and composite utility functions, each composed of a survival probability term and a component cost term. We present a two-level characterization of the correlations within the infrastructure: (i the aggregate failure correlation function specifies the infrastructure failure probability given the failure of an individual system or network, and (ii the survival probabilities of the systems and network satisfy first-order differential conditions that capture the component-level correlations using multiplier functions. We derive Nash equilibrium conditions that provide expressions for individual system survival probabilities and also the expected infrastructure capacity specified by the total number of operational components. We apply these results to derive and analyze defense strategies for distributed cloud computing infrastructures using cyber-physical models.

  19. Defense Strategies for Asymmetric Networked Systems with Discrete Components.

    Science.gov (United States)

    Rao, Nageswara S V; Ma, Chris Y T; Hausken, Kjell; He, Fei; Yau, David K Y; Zhuang, Jun

    2018-05-03

    We consider infrastructures consisting of a network of systems, each composed of discrete components. The network provides the vital connectivity between the systems and hence plays a critical, asymmetric role in the infrastructure operations. The individual components of the systems can be attacked by cyber and physical means and can be appropriately reinforced to withstand these attacks. We formulate the problem of ensuring the infrastructure performance as a game between an attacker and a provider, who choose the numbers of the components of the systems and network to attack and reinforce, respectively. The costs and benefits of attacks and reinforcements are characterized using the sum-form, product-form and composite utility functions, each composed of a survival probability term and a component cost term. We present a two-level characterization of the correlations within the infrastructure: (i) the aggregate failure correlation function specifies the infrastructure failure probability given the failure of an individual system or network, and (ii) the survival probabilities of the systems and network satisfy first-order differential conditions that capture the component-level correlations using multiplier functions. We derive Nash equilibrium conditions that provide expressions for individual system survival probabilities and also the expected infrastructure capacity specified by the total number of operational components. We apply these results to derive and analyze defense strategies for distributed cloud computing infrastructures using cyber-physical models.

  20. Process and apparatus for fractionating close-boiling components of a multi-component system

    International Nuclear Information System (INIS)

    Tsao, U.

    1983-01-01

    A process and apparatus are described for the fractionation of close-boiling components of a multi-component system comprising at least two fractionation columns A, B in series having a plurality of equilibrium stages in which the vapor stream from a downstream fractionation column B is compressed by a compressor and passed into a lower portion of a preceding fractionation column A and a liquid bottom stream from any one of said columns except the last is expanded by an orifice sufficiently to convey the resulting liquid-vapor mixture to the upper portion of the next fractionation column B. In a particularly preferred embodiment, the compressed overhead vapor stream is passed in heat transfer relationship to a liquid stream withdrawn from the preceding fractionation column A prior to introduction into the lower portion of such preceding fractionation column A. In one of the claims, the multi-component close-boiling system is a deuterium oxide-water solution. (author)

  1. Protective role of complement C3 against cytokine-mediated beta cell apoptosis

    DEFF Research Database (Denmark)

    Dos Santos, R. S.; Marroqui, L.; Grieco, F. A.

    2017-01-01

    Background and aims: Type 1 diabetes is a chronic autoimmune disease characterized by pancreatic islet inflammation and β-cell destruction by pro-inflammatory cytokines and other mediators. The complement system, a major component of the immune system, has been recently shown to also act in metab...... in metabolic organs, such as liver, adipose tissue, and pancreas. In the present study we identified complement C3 as an important hub of a cytokine-modified complement network in human islets and characterized the role of C3 in β-cell survival....

  2. Applying of component system development in object methodology

    Directory of Open Access Journals (Sweden)

    Milan Mišovič

    2013-01-01

    Full Text Available In the last three decades, the concept and implementation of component-based architectures have been promoted in software systems creation. Increasingly complex demands are placed on the software component systems, in particular relating to the dynamic properties. The emergence of such requirements has been gradually enforced by the practice of development and implementation of these systems, especially for information systems software.Just the information systems (robust IS of different types require that target software meets their requirements. Among other things, we mean primarily the adaptive processes of different domains, high distributives due to the possibilities of the Internet 2.0, acceptance of high integrity of life domains (process, data and communications integrity, scalability, and flexible adaptation to process changes, a good context for external devices and transparent structure of the sub-process modules and architectural units.Of course, the target software of required qualities and the type robust cannot be a monolith. As commonly known, development of design toward information systems software has clearly come to the need for the software composition of completely autonomous, but cooperating architectural units that communicate with each other using messages of prescribed formats.Although for such units there were often used the so called subsystems and modules, see (Jac, Boo, Rumbo, 1998 and (Arlo, Neus, 2007, their abstraction being gradually enacted as the term component. In other words, the subsystems and modules are specific types of components.In (Král, Žeml, 2000 and (Král, Žeml, 2003 there are considered two types of target software of information systems. The first type – there are SWC (Software Components, composed of permanently available components, which are thought as services – Confederate software. The second type – SWA (Software Alliance, called semi Confederate, formed during the run-time of the

  3. System diagnostics using qualitative analysis and component functional classification

    International Nuclear Information System (INIS)

    Reifman, J.; Wei, T.Y.C.

    1993-01-01

    A method for detecting and identifying faulty component candidates during off-normal operations of nuclear power plants involves the qualitative analysis of macroscopic imbalances in the conservation equations of mass, energy and momentum in thermal-hydraulic control volumes associated with one or more plant components and the functional classification of components. The qualitative analysis of mass and energy is performed through the associated equations of state, while imbalances in momentum are obtained by tracking mass flow rates which are incorporated into a first knowledge base. The plant components are functionally classified, according to their type, as sources or sinks of mass, energy and momentum, depending upon which of the three balance equations is most strongly affected by a faulty component which is incorporated into a second knowledge base. Information describing the connections among the components of the system forms a third knowledge base. The method is particularly adapted for use in a diagnostic expert system to detect and identify faulty component candidates in the presence of component failures and is not limited to use in a nuclear power plant, but may be used with virtually any type of thermal-hydraulic operating system. 5 figures

  4. Ventilation systems and components of nuclear power plants

    International Nuclear Information System (INIS)

    1997-01-01

    The most important radiation and nuclear safety requirements for the design and manufacture of nuclear power plant ventilation systems and components are presented in the guide. Also the regulatory activities of the Finnish Centre for Radiation and Nuclear Safety (STUK) as regards the ventilation systems and components are explained. Documents and data which shall be submitted to STUK during the various phases of the regulatory procedure relating to the design, construction, commissioning and operation of the nuclear power plants are presented. (13 refs.)

  5. Investigation on Prefabricated Building System Skilled Component Installers

    OpenAIRE

    Nurul Rezuana Buyung; Md Azree Othuman Mydin; Abdul Naser Abdul Ghani

    2013-01-01

    In the face of an increasingly challenging era of globalization, skills and new equipments which includes prefabricated building components, known as Industrialized Building System (IBS) has been introduced towards achievement of sustainable construction. IBS is a construction system in which the components are manufactured in a factory, on or off site, positioned and assembled into complete structures with minimal additional site work. IBS requires high construction precisi...

  6. New perspectives on mannan-binding lectin-mediated complement activation

    DEFF Research Database (Denmark)

    Degn, Søren Egedal; Thiel, Steffen; Jensenius, Jens Christian

    2007-01-01

    The complement system is an important part of the innate immune system, mediating several major effector functions and modulating adaptive immune responses. Three complement activation pathways exist: the classical pathway (CP), the alternative pathway (AP), and the lectin pathway (LP). The LP......, allowing C3 activation in the absence of components otherwise believed critical. The classical bypass pathways are dependent on C1 and components of the AP. A recent study has shown the existence also of a lectin bypass pathway dependent on mannan-binding lectin (MBL) and AP components. The emerging...

  7. Computers as components principles of embedded computing system design

    CERN Document Server

    Wolf, Marilyn

    2012-01-01

    Computers as Components: Principles of Embedded Computing System Design, 3e, presents essential knowledge on embedded systems technology and techniques. Updated for today's embedded systems design methods, this edition features new examples including digital signal processing, multimedia, and cyber-physical systems. Author Marilyn Wolf covers the latest processors from Texas Instruments, ARM, and Microchip Technology plus software, operating systems, networks, consumer devices, and more. Like the previous editions, this textbook: Uses real processors to demonstrate both technology and tec

  8. Lessons learned from mice deficient in lectin complement pathway molecules

    DEFF Research Database (Denmark)

    Genster, Ninette; Takahashi, Minoru; Sekine, Hideharu

    2014-01-01

    in turn activate downstream complement components, ultimately leading to elimination of the pathogen. Mice deficient in the key molecules of lectin pathway of complement have been generated in order to build knowledge of the molecular mechanisms of the lectin pathway in health and disease. Despite......The lectin pathway of the complement system is initiated when the pattern-recognition molecules, mannose-binding lectin (MBL), ficolins or collectin-11, bind to invading pathogens or damaged host cells. This leads to activation of MBL/ficolin/collectin-11 associated serine proteases (MASPs), which...... differences in the genetic arrangements of murine and human orthologues of lectin pathway molecules, the knockout mice have proven to be valuable models to explore the effect of deficiency states in humans. In addition, new insight and unexpected findings on the diverse roles of lectin pathway molecules...

  9. GLUCOCORTICOSTEROIDS' EFFECT UPON THE COMPLEMENT LEVEL

    Directory of Open Access Journals (Sweden)

    Voja Pavlovic

    2001-03-01

    Full Text Available The effect of high doses of cortisol upon the level of the overall complements'hemolytic activity and particular complements' components is studies. The experimentsinvolved guinea pigs of male sex of the body mass from 300 to 400 g, namelythose that have not been treated by anything so far. The doses of hydrocortisone(Hemofarm DD were also used for the experiment. The overall complements'activity was determined by testing the capabilities of a series of various solutions ofthe guinea pigs' serum to separate sheep erythrocytes that were made sensitive byrabbit anti-erythrocyte antibodies. The determination of the C1, C2, C3 and C4complements' components was done by the method of the quantitative diffusion ofthe radial type by using the Partigen blocks Behringwerke AG. The series comprised25 guinea pigs of male sex. The low cortisol level rapidly increase the overallhemolytic activity of the complements of the C1 est erase concentration. Along withthe cortisol dose increase the overall hemolytic complements' activity is dropping aswell as that of the C1, C2, C3 and C4 complements' components.

  10. Components and system tests on the RFX toroidal power supply

    International Nuclear Information System (INIS)

    Toigo, V.; Zanotto, L.; Gaio, E.; Perna, M.; Bordignon, P.; Coffetti, A.; Novaro, R.; Bertolotto, P.; Rinaldi, E.; Villa, G.

    2005-01-01

    The paper deals with the component and system tests performed on the new toroidal power supply system of the RFX experiment. The high technological innovation of the system required a deep experimental characterization and validation campaign; special factory tests were performed on prototypes of single components aimed at verifying the most critical design aspects. Consequently an articulated series of tests were performed, based on a step-by-step approach to achieve the desired coordinate operation of the whole system. The test procedures and the most significant results are described in the paper

  11. Robustness of Component Models in Energy System Simulators

    DEFF Research Database (Denmark)

    Elmegaard, Brian

    2003-01-01

    During the development of the component-based energy system simulator DNA (Dynamic Network Analysis), several obstacles to easy use of the program have been observed. Some of these have to do with the nature of the program being based on a modelling language, not a graphical user interface (GUI......). Others have to do with the interaction between models of the nature of the substances in an energy system (e.g., fuels, air, flue gas), models of the components in a system (e.g., heat exchangers, turbines, pumps), and the solver for the system of equations. This paper proposes that the interaction...

  12. Context sensitivity and ambiguity in component-based systems design

    Energy Technology Data Exchange (ETDEWEB)

    Bespalko, S.J.; Sindt, A.

    1997-10-01

    Designers of components-based, real-time systems need to guarantee to correctness of soft-ware and its output. Complexity of a system, and thus the propensity for error, is best characterized by the number of states a component can encounter. In many cases, large numbers of states arise where the processing is highly dependent on context. In these cases, states are often missed, leading to errors. The following are proposals for compactly specifying system states which allow the factoring of complex components into a control module and a semantic processing module. Further, the need for methods that allow for the explicit representation of ambiguity and uncertainty in the design of components is discussed. Presented herein are examples of real-world problems which are highly context-sensitive or are inherently ambiguous.

  13. LEDA RF distribution system design and component test results

    International Nuclear Information System (INIS)

    Roybal, W.T.; Rees, D.E.; Borchert, H.L.; McCarthy, M.; Toole, L.

    1998-01-01

    The 350 MHz and 700 MHz RF distribution systems for the Low Energy Demonstration Accelerator (LEDA) have been designed and are currently being installed at Los Alamos National Laboratory. Since 350 MHz is a familiar frequency used at other accelerator facilities, most of the major high-power components were available. The 700 MHz, 1.0 MW, CW RF delivery system designed for LEDA is a new development. Therefore, high-power circulators, waterloads, phase shifters, switches, and harmonic filters had to be designed and built for this applications. The final Accelerator Production of Tritium (APT) RF distribution systems design will be based on much of the same technology as the LEDA systems and will have many of the RF components tested for LEDA incorporated into the design. Low power and high-power tests performed on various components of these LEDA systems and their results are presented here

  14. Commercial Off-the-Shelf (COTS) Components and Enterprise Component Information System (eCIS)

    Energy Technology Data Exchange (ETDEWEB)

    John Minihan; Ed Schmidt; Greg Enserro; Melissa Thompson

    2008-06-30

    The purpose of the project was to develop the processes for using commercial off-the-shelf (COTS) parts for WR production and to put in place a system for implementing the data management tools required to disseminate, store, track procurement, and qualify vendors. Much of the effort was devoted to determining if the use of COTS parts was possible. A basic question: How does the Nuclear Weapons Complex (NWC) begin to use COTS in the weapon Stockpile Life Extension Programs with high reliability, affordability, while managing risk at acceptable levels? In FY00, it was determined that a certain weapon refurbishment program could not be accomplished without the use of COTS components. The elements driving the use of COTS components included decreased cost, greater availability, and shorter delivery time. Key factors that required implementation included identifying the best suppliers and components, defining life cycles and predictions of obsolescence, testing the feasibility of using COTS components with a test contractor to ensure capability, as well as quality and reliability, and implementing the data management tools required to disseminate, store, track procurement, and qualify vendors. The primary effort of this project then was to concentrate on the risks involved in the use of COTS and address the issues of part and vendor selection, procurement and acceptance processes, and qualification of the parts via part and sample testing. The Enterprise Component Information System (eCIS) was used to manage the information generated by the COTS process. eCIS is a common interface for both the design and production of NWC components and systems integrating information between SNL National Laboratory (SNL) and the Kansas City Plant (KCP). The implementation of COTS components utilizes eCIS from part selection through qualification release. All part related data is linked across an unclassified network for access by both SNL and KCP personnel. The system includes not

  15. Calculation and experimental investigation of multi-component ceramic systems

    International Nuclear Information System (INIS)

    Rother, M.

    1994-12-01

    This work shows a way to combine thermodynamic calculations and experiments in order to get useful information on the constitution of metal/non-metal systems. Many data from literature are critically evaluated and used as a basis for experiments and calculations. The following multi-component systems are treated: 1. Multi-component systems of 'ceramic' materials with partially metallic bonding (carbides, nitrides, oxides, borides, carbonitrides, borocarbides, oxinitrides of the 4-8th transition group metals) 2. multi-component systems of non-metallic materials with dominant covalent bonding (SiC, Si 3 N 4 , SiB 6 , BN, Al 4 C 3 , Be 2 C) 3. multi-component systems of non-metallic materials with dominant heteropolar bonding (Al 2 O 3 , TiO 2 , BeO, SiO 2 , ZrO 2 ). The interactions between 1. and 2., 2. and 3., 1. and 3. are also considered. The latest commercially available programmes for the calculation of thermodynamical equilibria and phase diagrams are evaluated and compared considering their facilities and limits. New phase diagrams are presented for many presently unknown multi-component systems; partly known systems are completed on the basis of selected thermodynamic data. The calculations are verified by experimental investigations (metallurgical and powder technology methods). Altogether 690 systems are evaluated, 126 are calculated for the first time and 52 systems are experimentally verified. New data for 60 ternary phases are elaborated by estimating the data limits for the Gibbs energy values. A synthesis of critical evaluation of literature, calculations and experiments leads to new important information about equilibria and reaction behaviour in multi-component systems. This information is necessary to develop new stable and metastable materials. (orig./MM) [de

  16. Subversion of complement by hematophagous parasites.

    Science.gov (United States)

    Schroeder, Hélène; Skelly, Patrick J; Zipfel, Peter F; Losson, Bertrand; Vanderplasschen, Alain

    2009-01-01

    The complement system is a crucial part of innate and adaptive immunity which exerts a significant evolutionary pressure on pathogens. It has selected for those pathogens, mainly microorganisms but also parasites, that have evolved countermeasures. The characterization of how pathogens evade complement attack is a rapidly developing field of current research. In recent years, multiple complement evasion strategies have been characterized. In this review, we focus on complement escape mechanisms expressed by hematophagous parasites, a heterogeneous group of metazoan parasites that share the property of ingesting the whole blood of their host. Complement inhibition is crucial for parasite survival within the host tissue or to facilitate blood feeding. Finally, complement inhibition by hematophagous parasites may also contribute to their success as pathogen vectors.

  17. Component configuration control system development at EBR-II

    International Nuclear Information System (INIS)

    Monson, L.R.; Stratton, R.C.

    1984-01-01

    One ofthe major programs being pursued by the EBR-II Division of Argonne National Laboratory is to improve the reliability of plant control and protection systems. This effort involves looking closely at the present state of the art and needs associated with plant diagnostic, control and protection systems. One of the areas of development at EBR-II involves a component configuration control system (CCCS). This system is a computerized control and planning aid for the nuclear power operator

  18. Complement anaphylatoxins as immune regulators in cancer

    OpenAIRE

    Sayegh, Eli T; Bloch, Orin; Parsa, Andrew T

    2014-01-01

    The role of the complement system in innate immunity is well characterized. However, a recent body of research implicates the complement anaphylatoxins C3a and C5a as insidious propagators of tumor growth and progression. It is now recognized that certain tumors elaborate C3a and C5a and that complement, as a mediator of chronic inflammation and regulator of immune function, may in fact foster rather than defend against tumor growth. A putative mechanism for this function is complement-mediat...

  19. HTR-E project. High-temperature components and systems

    International Nuclear Information System (INIS)

    Breuil, E.; Exner, R.

    2002-01-01

    The HTR-E European project (four years project) is proposed for the 5th Framework Programme and concerns the technical developments needed for the innovative components of a modern HTR with a direct cycle. These components have been selected with reference to the present projects (GT-MHR, PBMR): (1) the helium turbine, the recuperator heat exchanger, the electro-magnetic bearings and the helium rotating seal; (2) the tribology. Sliding innovative components in helium environment are particularly concerned. (3) the helium purification system. Recommendations on impurities contents have to be provided in accordance with the materials proposed for the innovative components. The main outcomes expected from the HTR-E project are the design recommendations and identification of further R and D needs for these components. This will be based: (1) on experience feedback from European past helium test loops and reactors; (2) on design studies, thermal-hydraulic and structural analyses; (3) and on experimental tests

  20. NSSS Component Control System Design of Integral Reactor

    International Nuclear Information System (INIS)

    Lee, Joon Koo; Kwon, Ho Je; Jeong, Kwong Il; Park, Heui Youn; Koo, In Soo

    2005-01-01

    MMIS(Man Machine Interface System) of an integral reactor is composed of a Control Room, Plant Protection System, Control System and Monitoring System which are related with the overall plant operation. MMIS is being developed with a new design concept and digital technology to reduce the Human Factor Error and improve the systems' safety, reliability and availability. And CCS(component control system) is also being developed with a new design concept and digital hardware technology A fully digitalized system and design concept are introduced in the NSSS CCS

  1. Applying of component system development in object methodology, case study

    Directory of Open Access Journals (Sweden)

    Milan Mišovič

    2013-01-01

    Full Text Available To create computarization target software as a component system has been a very strong requirement for the last 20 years of software developing. Finally, the architectural components are self-contained units, presenting not only partial and overall system behavior, but also cooperating with each other on the basis of their interfaces. Among others, components have allowed flexible modification of processes the behavior of which is the foundation of components behavior without changing the life of the component system. On the other hand, the component system makes it possible, at design time, to create numerous new connections between components and thus creating modified system behaviors. This all enables the company management to perform, at design time, required behavioral changes of processes in accordance with the requirements of changing production and market.The development of software which is generally referred to as SDP (Software Development Process contains two directions. The first one, called CBD (Component–Based Development, is dedicated to the development of component–based systems CBS (Component–based System, the second target is the development of software under the influence of SOA (Service–Oriented Architecture. Both directions are equipped with their different development methodologies. The subject of this paper is only the first direction and application of development of component–based systems in its object–oriented methodologies. The requirement of today is to carry out the development of component-based systems in the framework of developed object–oriented methodologies precisely in the way of a dominant style. In some of the known methodologies, however, this development is not completely transparent and is not even recognized as dominant. In some cases, it is corrected by the special meta–integration models of component system development into an object methodology.This paper presents a case study

  2. Fuel-cell-system and its components for mobile application

    Energy Technology Data Exchange (ETDEWEB)

    Venturi, Massimo [NuCellSys GmbH, Kirchheim/Teck-Nabern (Germany)

    2013-06-01

    In the past years the development of fuel cell systems for mobile applications has made significant progress in power density, performance and robustness. For a successful market introduction the cost of the fuel system powertrain needs to be competitive to diesel hybrid engine. The current development activities are therefore focusing on cost reduction. There are 3 major areas for cost reduction: functional integration, materials and design, supplier competitiveness and volume. Today unique fuel cell system components are developed by single suppliers, which lead to a monopoly. In the future the components will be developed at multiple suppliers to achieve a competitor situation, which will further reduce the component cost. Using all these cost reduction measures the fuel cell system will become a competitive alternative drive train. (orig.)

  3. Energy dependence of critical state of single-component systems

    International Nuclear Information System (INIS)

    Volchenkova, R.A.

    1985-01-01

    Equations of critical states of the single-component systems: Psub(cr)(/Psub(o)=(Tsub(cr)/Tsub(o))x0.73, Tsub(cr)=K(Tsub(boil))sup(1.116) and Hsub(cr)(/Hsub(B)=Tsub(sr)/Tsub(B))sup(1.48) where Tsub(B)=1K, Hsub(B)-2 kcal/g-at, K-dimension factor are presented. It is shown that the revealed dependence Hsub(cr)=H(Tsub(cr)) is an energy boundary of a liquid-vapour phase state of the single-component systems beyond limits of which difference between liquid and vapour phases vanishes in increasing the system energy content. The given equations of state are true for all the single-component systems and permit to consider physicomechanical properties of substances in dynamic state depending on external conditions. Critical temperatures and dependences for elements from the most fusible He to infusible W and Re have been calculated

  4. In silico dissection of Type VII Secretion System components across

    Indian Academy of Sciences (India)

    Type VII Secretion System (T7SS) is one of the factors involved in virulence of Mycobacteriun tuberculosis H37Rv. Numerous research efforts have been made in the last decade towards characterizing the components of this secretion system. An extensive genome-wide analysis through compilation of isolated information ...

  5. Integrity of neutron-absorbing components of LWR fuel systems

    International Nuclear Information System (INIS)

    Bailey, W.J.; Berting, F.M.

    1991-03-01

    A study of the integrity and behavior of neutron-absorbing components of light-water (LWR) fuel systems was performed by Pacific Northwest Laboratory (PNL) and sponsored by the US Department of Energy (DOE). The components studies include control blades (cruciforms) for boiling-water reactors (BWRs) and rod cluster control assemblies for pressurized-water reactors (PWRs). The results of this study can be useful for understanding the degradation of neutron-absorbing components and for waste management planning and repository design. The report includes examples of the types of degradation, damage, or failures that have been encountered. Conclusions and recommendations are listed. 84 refs

  6. Comparison of Component Frameworks for Real-Time Embedded Systems

    Czech Academy of Sciences Publication Activity Database

    Pop, T.; Hnětynka, P.; Hošek, P.; Malohlava, M.; Bureš, Tomáš

    2014-01-01

    Roč. 40, č. 1 (2014), s. 127-170 ISSN 0219-1377 Grant - others:GA AV ČR(CZ) GAP202/11/0312; GA UK(CZ) Project 378111; UK(CZ) SVV-2013- 267312 Keywords : component-based development * component frameworks * real-time and embedded systems Subject RIV: JC - Computer Hardware ; Software Impact factor: 1.782, year: 2014

  7. Complement activation on the surface of cell-derived microparticles during cardiac surgery with cardiopulmonary bypass - is retransfusion of pericardial blood harmful?

    Science.gov (United States)

    Biró, E; van den Goor, J M; de Mol, B A; Schaap, M C; Ko, L-Y; Sturk, A; Hack, C E; Nieuwland, R

    2011-01-01

    To investigate whether cell-derived microparticles play a role in complement activation in pericardial blood of patients undergoing cardiac surgery with cardiopulmonary bypass (CPB) and whether microparticles in pericardial blood contribute to systemic complement activation upon retransfusion. Pericardial blood of 13 patients was retransfused in 9 and discarded in 4 cases. Microparticles were isolated from systemic blood collected before anesthesia (T1) and at the end of CPB (T2), and from pericardial blood. The microparticles were analyzed by flow cytometry for bound complement components C1q, C4 and C3, and bound complement activator molecules C-reactive protein (CRP), serum amyloid P-component (SAP), immunoglobulin (Ig)M and IgG. Fluid-phase complement activation products (C4b/c, C3b/c) and activator molecules were determined by ELISA. Compared with systemic T1 blood, pericardial blood contained increased C4b/c and C3b/c, and increased levels of microparticles with bound complement components. In systemic T1 samples, microparticle-bound CRP, whereas in pericardial blood, microparticle-bound SAP and IgM were associated with complement activation. At the end of CPB, increased C3b/c (but not C4b/c) was present in systemic T2 blood compared with T1, while concentrations of microparticles binding complement components and of those binding complement activator molecules were similar. Concentrations of fluid-phase complement activation products and microparticles were similar in patients whether or not retransfused with pericardial blood. In pericardial blood of patients undergoing cardiac surgery with CPB, microparticles contribute to activation of the complement system via bound SAP and IgM. Retransfusion of pericardial blood, however, does not contribute to systemic complement activation.

  8. The Node Monitoring Component of a Scalable Systems Software Environment

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Samuel James [Iowa State Univ., Ames, IA (United States)

    2006-01-01

    This research describes Fountain, a suite of programs used to monitor the resources of a cluster. A cluster is a collection of individual computers that are connected via a high speed communication network. They are traditionally used by users who desire more resources, such as processing power and memory, than any single computer can provide. A common drawback to effectively utilizing such a large-scale system is the management infrastructure, which often does not often scale well as the system grows. Large-scale parallel systems provide new research challenges in the area of systems software, the programs or tools that manage the system from boot-up to running a parallel job. The approach presented in this thesis utilizes a collection of separate components that communicate with each other to achieve a common goal. While systems software comprises a broad array of components, this thesis focuses on the design choices for a node monitoring component. We will describe Fountain, an implementation of the Scalable Systems Software (SSS) node monitor specification. It is targeted at aggregate node monitoring for clusters, focusing on both scalability and fault tolerance as its design goals. It leverages widely used technologies such as XML and HTTP to present an interface to other components in the SSS environment.

  9. On fault propagation in deterioration of multi-component systems

    International Nuclear Information System (INIS)

    Liang, Zhenglin; Parlikad, Ajith Kumar; Srinivasan, Rengarajan; Rasmekomen, Nipat

    2017-01-01

    In extant literature, deterioration dependence among components can be modelled as inherent dependence and induced dependence. We find that the two types of dependence may co-exist and interact with each other in one multi-component system. We refer to this phenomenon as fault propagation. In practice, a fault induced by the malfunction of a non-critical component may further propagate through the dependence amongst critical components. Such fault propagation scenario happens in industrial assets or systems (bridge deck, and heat exchanging system). In this paper, a multi-layered vector-valued continuous-time Markov chain is developed to capture the characteristics of fault propagation. To obtain the mathematical tractability, we derive a partitioning rule to aggregate states with the same characteristics while keeping the overall aging behaviour of the multi-component system. Although the detailed information of components is masked by aggregated states, lumpability is attainable with the partitioning rule. It means that the aggregated process is stochastically equivalent to the original one and retains the Markov property. We apply this model on a heat exchanging system in oil refinery company. The results show that fault propagation has a more significant impact on the system's lifetime comparing with inherent dependence and induced dependence. - Highlights: • We develop a vector value continuous-time Markov chain to model the meta-dependent characteristic of fault propagation. • A partitioning rule is derived to reduce the state space and attain lumpability. • The model is applied on analysing the impact of fault propagation in a heat exchanging system.

  10. Complement pathways and meningococcal disease : diagnostic aspects

    DEFF Research Database (Denmark)

    Sjöholm, A G; Truedsson, L; Jensenius, Jens Christian

    2001-01-01

    Complement is an immunological effector system that bridges innate and acquired immunity in several ways. There is a striking association between susceptibility to meningococcal disease and various forms of complement deficiency (1,2). In defense against bacterial infection, the most important fu...

  11. Towards a Component Based Model for Database Systems

    Directory of Open Access Journals (Sweden)

    Octavian Paul ROTARU

    2004-02-01

    Full Text Available Due to their effectiveness in the design and development of software applications and due to their recognized advantages in terms of reusability, Component-Based Software Engineering (CBSE concepts have been arousing a great deal of interest in recent years. This paper presents and extends a component-based approach to object-oriented database systems (OODB introduced by us in [1] and [2]. Components are proposed as a new abstraction level for database system, logical partitions of the schema. In this context, the scope is introduced as an escalated property for transactions. Components are studied from the integrity, consistency, and concurrency control perspective. The main benefits of our proposed component model for OODB are the reusability of the database design, including the access statistics required for a proper query optimization, and a smooth information exchange. The integration of crosscutting concerns into the component database model using aspect-oriented techniques is also discussed. One of the main goals is to define a method for the assessment of component composition capabilities. These capabilities are restricted by the component’s interface and measured in terms of adaptability, degree of compose-ability and acceptability level. The above-mentioned metrics are extended from database components to generic software components. This paper extends and consolidates into one common view the ideas previously presented by us in [1, 2, 3].[1] Octavian Paul Rotaru, Marian Dobre, Component Aspects in Object Oriented Databases, Proceedings of the International Conference on Software Engineering Research and Practice (SERP’04, Volume II, ISBN 1-932415-29-7, pages 719-725, Las Vegas, NV, USA, June 2004.[2] Octavian Paul Rotaru, Marian Dobre, Mircea Petrescu, Integrity and Consistency Aspects in Component-Oriented Databases, Proceedings of the International Symposium on Innovation in Information and Communication Technology (ISIICT

  12. Protective function of complement against alcohol-induced rat liver damage.

    Science.gov (United States)

    Bykov, Igor L; Väkevä, Antti; Järveläinen, Harri A; Meri, Seppo; Lindros, Kai O

    2004-11-01

    The complement system can promote tissue damage or play a homeostatic role in the clearance and disposal of damaged tissue. We assessed the role of the terminal complement pathway in alcohol-induced liver damage in complement C6 (C6-/-) genetically deficient rats. C6-/- and corresponding C6+/+ rats were continuously exposed to ethanol by feeding ethanol-supplemented liquid diet for six weeks. Liver samples were analyzed for histopathology and complement component deposition by immunofluorescence microscopy. Prostaglandin E receptors and cytokine mRNA levels were analyzed by RT-PCR and plasma cytokines by ELISA. Deposition of complement components C1, C3, C8 and C9 was observed in C6+/+ rats, but not in C6-/- animals. The histopathological changes, the liver weight increase and the elevation of the plasma pro-/anti-inflammatory TNF-alpha/IL-10 ratio were, on the other hand, more marked in C6-/- rats. Furthermore, ethanol enhanced the hepatic mRNA expression of the prostaglandin E receptors EP2R and EP4R exclusively in the C6-/- rats. Our results indicate that a deficient terminal complement pathway predisposes to tissue injury and promotes a pro-inflammatory cytokine response. This suggests that an intact complement system has a protective function in the development of alcoholic liver damage.

  13. Complement anaphylatoxins as immune regulators in cancer.

    Science.gov (United States)

    Sayegh, Eli T; Bloch, Orin; Parsa, Andrew T

    2014-08-01

    The role of the complement system in innate immunity is well characterized. However, a recent body of research implicates the complement anaphylatoxins C3a and C5a as insidious propagators of tumor growth and progression. It is now recognized that certain tumors elaborate C3a and C5a and that complement, as a mediator of chronic inflammation and regulator of immune function, may in fact foster rather than defend against tumor growth. A putative mechanism for this function is complement-mediated suppression of immune effector cells responsible for immunosurveillance within the tumor microenvironment. This paradigm accords with models of immune dysregulation, such as autoimmunity and infectious disease, which have defined a pathophysiological role for abnormal complement signaling. Several types of immune cells express the cognate receptors for the complement anaphylatoxins, C3aR and C5aR, and demonstrate functional modulation in response to complement stimulation. In turn, impairment of antitumor immunity has been intimately tied to tumor progression in animal models of cancer. In this article, the literature was systematically reviewed to identify studies that have characterized the effects of the complement anaphylatoxins on the composition and function of immune cells within the tumor microenvironment. The search identified six studies based upon models of lymphoma and ovarian, cervical, lung, breast, and mammary cancer, which collectively support the paradigm of complement as an immune regulator in the tumor microenvironment. © 2014 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

  14. Genetic analysis of complement C1s deficiency associated with systemic lupus erythematosus highlights alternative splicing of normal C1s gene

    DEFF Research Database (Denmark)

    Amano, Mariane T; Ferriani, Virgínia P L; Florido, Marlene P C

    2008-01-01

    Deficiencies of complement proteins of the classical pathway are strongly associated with the development of autoimmune diseases. Deficiency of C1r has been observed to occur concomitantly with deficiency in C1s and 9 out of 15 reported cases presented systemic lupus erythematosus (SLE). Here, we...... describe a family in which all four children are deficient in C1s but only two of them developed SLE. Hemolytic activity mediated by the alternative and the lectin pathways were normal, but classical pathway activation was absent in all children's sera. C1s was undetectable, while in the parents' sera...

  15. Susceptibility to invasive meningococcal disease: polymorphism of complement system genes and Neisseria meningitidis factor H binding protein.

    Directory of Open Access Journals (Sweden)

    Declan T Bradley

    Full Text Available Neisseria meningitidis can cause severe infection in humans. Polymorphism of Complement Factor H (CFH is associated with altered risk of invasive meningococcal disease (IMD. We aimed to find whether polymorphism of other complement genes altered risk and whether variation of N. meningitidis factor H binding protein (fHBP affected the risk association.We undertook a case-control study with 309 European cases and 5,200 1958 Birth Cohort and National Blood Service cohort controls. We used additive model logistic regression, accepting P<0.05 as significant after correction for multiple testing. The effects of fHBP subfamily on the age at infection and severity of disease was tested using the independent samples median test and Student's T test. The effect of CFH polymorphism on the N. meningitidis fHBP subfamily was investigated by logistic regression and Chi squared test.Rs12085435 A in C8B was associated with odds ratio (OR of IMD (0.35 [95% CI 0.19-0.67]; P = 0.03 after correction. A CFH haplotype tagged by rs3753396 G was associated with IMD (OR 0.56 [95% CI 0.42-0.76], P = 1.6x10⁻⁴. There was no bacterial load (CtrA cycle threshold difference associated with carriage of this haplotype. Host CFH haplotype and meningococcal fHBP subfamily were not associated. Individuals infected with meningococci expressing subfamily A fHBP were younger than those with subfamily B fHBP meningococci (median 1 vs 2 years; P = 0.025.The protective CFH haplotype alters odds of IMD without affecting bacterial load for affected heterozygotes. CFH haplotype did not affect the likelihood of infecting meningococci having either fHBP subfamily. The association between C8B rs12085435 and IMD requires independent replication. The CFH association is of interest because it is independent of known functional polymorphisms in CFH. As fHBP-containing vaccines are now in use, relationships between CFH polymorphism and vaccine effectiveness and side-effects may become

  16. Integrated Evaluation of Closed Loop Air Revitalization System Components

    Science.gov (United States)

    Murdock, K.

    2010-01-01

    NASA s vision and mission statements include an emphasis on human exploration of space, which requires environmental control and life support technologies. This Contractor Report (CR) describes the development and evaluation of an Air Revitalization System, modeling and simulation of the components, and integrated hardware testing with the goal of better understanding the inherent capabilities and limitations of this closed loop system. Major components integrated and tested included a 4-Bed Modular Sieve, Mechanical Compressor Engineering Development Unit, Temperature Swing Adsorption Compressor, and a Sabatier Engineering and Development Unit. The requisite methodolgy and technical results are contained in this CR.

  17. Manual on maintenance of systems and components important to safety

    International Nuclear Information System (INIS)

    1986-01-01

    The Manual should serve as guidance at the plant management level for the maintenance of systems and components important to safety. It includes a detailed description of management systems, administrative controls and procedures. The Annexes contain examples of documents and practices adopted by Operating Organizations of some Member States. It is not the intention of this Manual to address the technical problem of how to maintain a particular component but rather to cover the programmatic aspects of maintenance. It also contains some aspects of surveillance and verification activities. The Manual makes only general statements about radiation protection provisions in connection with maintenance; detailed guidance can be found in other IAEA documents

  18. Removal of Shippingport Station primary system components and piping

    International Nuclear Information System (INIS)

    LaGuardia, T.S.; Lipsett, S.M.

    1987-01-01

    The dismantling workscope for the Shippingport Station Decommissioning Project was divided into subtasks to permit the work to be subcontracted to the maximum extent practicable. Major subtasks were identified and described by Activity specifications which could then be grouped into logical work packages to be put out for bid. Two of the largest dismantling work packages, removal of piping and components, were grouped together and designated as Activity Specifications 4 and 5. TLG Services, Inc. and Cleveland Wrecking Company formed a Joint Venture to perform this work during a two-year period at a cost of approximately $7 million. The major portions of this dismantling workscope are described. The primary system components within this workscope consist of the stainless steel reactor coolant piping, check valves, reactor coolant pumps, steam generators, and reactor purification demineralizers and coolers. The work performed, the heavy rigging preparations and procedures, the cutting tools used, component draining/capping techniques to prevent spills, contamination containment, airborne control techniques, and lessons learned during the removal of these primary system components are described. Summaries of crew size and composition, labor hours, duration hours and radiation exposure to workers are provided and discussed briefly. The successful completion of this work is evidence of the engineering, planning, equipment, materials and labor pool available to remove large, radioactively contaminated components safely. This experience will help decommissioning planners to prepare for the removal of reactor components in future decommissioning

  19. Water reuse systems: A review of the principal components

    Science.gov (United States)

    Lucchetti, G.; Gray, G.A.

    1988-01-01

    Principal components of water reuse systems include ammonia removal, disease control, temperature control, aeration, and particulate filtration. Effective ammonia removal techniques include air stripping, ion exchange, and biofiltration. Selection of a particular technique largely depends on site-specific requirements (e.g., space, existing water quality, and fish densities). Disease control, although often overlooked, is a major problem in reuse systems. Pathogens can be controlled most effectively with ultraviolet radiation, ozone, or chlorine. Simple and inexpensive methods are available to increase oxygen concentration and eliminate gas supersaturation, these include commercial aerators, air injectors, and packed columns. Temperature control is a major advantage of reuse systems, but the equipment required can be expensive, particularly if water temperature must be rigidly controlled and ambient air temperature fluctuates. Filtration can be readily accomplished with a hydrocyclone or sand filter that increases overall system efficiency. Based on criteria of adaptability, efficiency, and reasonable cost, we recommend components for a small water reuse system.

  20. An automatic chip structure optical inspection system for electronic components

    Science.gov (United States)

    Song, Zhichao; Xue, Bindang; Liang, Jiyuan; Wang, Ke; Chen, Junzhang; Liu, Yunhe

    2018-01-01

    An automatic chip structure inspection system based on machine vision is presented to ensure the reliability of electronic components. It consists of four major modules, including a metallographic microscope, a Gigabit Ethernet high-resolution camera, a control system and a high performance computer. An auto-focusing technique is presented to solve the problem that the chip surface is not on the same focusing surface under the high magnification of the microscope. A panoramic high-resolution image stitching algorithm is adopted to deal with the contradiction between resolution and field of view, caused by different sizes of electronic components. In addition, we establish a database to storage and callback appropriate parameters to ensure the consistency of chip images of electronic components with the same model. We use image change detection technology to realize the detection of chip images of electronic components. The system can achieve high-resolution imaging for chips of electronic components with various sizes, and clearly imaging for the surface of chip with different horizontal and standardized imaging for ones with the same model, and can recognize chip defects.

  1. Green IT engineering components, networks and systems implementation

    CERN Document Server

    Kondratenko, Yuriy; Kacprzyk, Janusz

    2017-01-01

    This book presents modern approaches to improving the energy efficiency, safety and environmental performance of industrial processes and products, based on the application of advanced trends in Green Information Technologies (IT) Engineering to components, networks and complex systems (software, programmable and hardware components, communications, Cloud and IoT-based systems, as well as IT infrastructures). The book’s 16 chapters, prepared by authors from Greece, Malaysia, Russia, Slovakia, Ukraine and the United Kingdom, are grouped into four sections: (1) The Green Internet of Things, Cloud Computing and Data Mining, (2) Green Mobile and Embedded Control Systems, (3) Green Logic and FPGA Design, and (4) Green IT for Industry and Smart Grids. The book will motivate researchers and engineers from different IT domains to develop, implement and propagate green values in complex systems. Further, it will benefit all scientists and graduate students pursuing research in computer science with a focus on green ...

  2. Optimal maintenance of multi-component systems: a review

    NARCIS (Netherlands)

    R.P. Nicolai (Robin); R. Dekker (Rommert)

    2006-01-01

    textabstractIn this article we give an overview of the literature on multi-component maintenance optimization. We focus on work appearing since the 1991 survey "A survey of maintenance models for multi-unit systems" by Cho and Parlar. This paper builds forth on the review article by Dekker et al.

  3. Plant systems/components modularization study. Final report. [PWR

    Energy Technology Data Exchange (ETDEWEB)

    1977-07-01

    The final results are summarized of a Plant Systems/Components Modularization Study based on Stone and Webster's Pressurized Water Reactor Reference Design. The program has been modified to include evaluation of the most promising areas for modular consideration based on the level of the Sundesert Project engineering design completion and the feasibility of their incorporation into the plant construction effort.

  4. Predicting phase equilibria in one-component systems

    Science.gov (United States)

    Korchuganova, M. R.; Esina, Z. N.

    2015-07-01

    It is shown that Simon equation coefficients for n-alkanes and n-alcohols can be modeled using critical and triple point parameters. Predictions of the phase liquid-vapor, solid-vapor, and liquid-solid equilibria in one-component systems are based on the Clausius-Clapeyron relation, Van der Waals and Simon equations, and the principle of thermodynamic similarity.

  5. Plant systems/components modularization study. Final report

    International Nuclear Information System (INIS)

    1977-07-01

    The final results are summarized of a Plant Systems/Components Modularization Study based on Stone and Webster's Pressurized Water Reactor Reference Design. The program has been modified to include evaluation of the most promising areas for modular consideration based on the level of the Sundesert Project engineering design completion and the feasibility of their incorporation into the plant construction effort

  6. Simulating the DIRCM engagement: component and system level performance

    CSIR Research Space (South Africa)

    Willers, CJ

    2012-11-01

    Full Text Available , spectrally matched, pyrophoric. Jammers with arc lamps and steered (directional) beams. Early directional laser beams: soft- kill - jamming & dazzling. Single head, complex gimbals. Advanced direction- al laser beams: hard kill - power levels cause damage... countermeasures (DIRCM), employing high power lamps or lasers as sources of infrared energy. The larger aircraft self-protection scenario, comprising the missile, aircraft and DIRCM hardware is a complex system. In this system, each component presents major...

  7. Modelling safety of multistate systems with ageing components

    Energy Technology Data Exchange (ETDEWEB)

    Kołowrocki, Krzysztof; Soszyńska-Budny, Joanna [Gdynia Maritime University, Department of Mathematics ul. Morska 81-87, Gdynia 81-225 Poland (Poland)

    2016-06-08

    An innovative approach to safety analysis of multistate ageing systems is presented. Basic notions of the ageing multistate systems safety analysis are introduced. The system components and the system multistate safety functions are defined. The mean values and variances of the multistate systems lifetimes in the safety state subsets and the mean values of their lifetimes in the particular safety states are defined. The multi-state system risk function and the moment of exceeding by the system the critical safety state are introduced. Applications of the proposed multistate system safety models to the evaluation and prediction of the safty characteristics of the consecutive “m out of n: F” is presented as well.

  8. Modelling safety of multistate systems with ageing components

    International Nuclear Information System (INIS)

    Kołowrocki, Krzysztof; Soszyńska-Budny, Joanna

    2016-01-01

    An innovative approach to safety analysis of multistate ageing systems is presented. Basic notions of the ageing multistate systems safety analysis are introduced. The system components and the system multistate safety functions are defined. The mean values and variances of the multistate systems lifetimes in the safety state subsets and the mean values of their lifetimes in the particular safety states are defined. The multi-state system risk function and the moment of exceeding by the system the critical safety state are introduced. Applications of the proposed multistate system safety models to the evaluation and prediction of the safty characteristics of the consecutive “m out of n: F” is presented as well.

  9. Systems of independent Markov components and their transient behavior

    International Nuclear Information System (INIS)

    Keilson, J.

    1975-01-01

    The transient behavior of redundant systems of independent, repairable, memoryless (two state) components is studied. Four failure times for such systems are considered, each an exit time from the set of working states for initial system conditions of interest: the failure time from the perfect state, the post-recovery exit time, the ergodic exit time, and the quasi-stationary exit time. The structure of these failure time distributions and their interrelation are discussed and asymptotic estimates and bounds for their expectations are presented. When such systems have high reliability, the failure time distributions are approximately exponential and asymptotically equivalent

  10. Quantifying the value of investing in distributed natural gas and renewable electricity systems as complements: Applications of discounted cash flow and real options analysis with stochastic inputs

    International Nuclear Information System (INIS)

    Pless, Jacquelyn; Arent, Douglas J.; Logan, Jeffrey; Cochran, Jaquelin; Zinaman, Owen

    2016-01-01

    One energy policy objective in the United States is to promote the adoption of technologies that provide consumers with stable, secure, and clean energy. Recent work provides anecdotal evidence of natural gas (NG) and renewable electricity (RE) synergies in the power sector, however few studies quantify the value of investing in NG and RE systems together as complements. This paper uses discounted cash flow analysis and real options analysis to value hybrid NG-RE systems in distributed applications, focusing on residential and commercial projects assumed to be located in the states of New York and Texas. Technology performance and operational risk profiles are modeled at the hourly level to capture variable RE output and NG prices are modeled stochastically as geometric Ornstein-Uhlenbeck (OU) stochastic processes to capture NG price uncertainty. The findings consistently suggest that NG-RE hybrid distributed systems are more favorable investments in the applications studied relative to their single-technology alternatives when incentives for renewables are available. In some cases, NG-only systems are the favorable investments. Understanding the value of investing in NG-RE hybrid systems provides insights into one avenue towards reducing greenhouse gas emissions, given the important role of NG and RE in the power sector. - Highlights: • Natural gas and renewable electricity can be viewed as complements. • We model hybrid natural gas and renewable electricity systems at the hourly level. • We incorporate variable renewable power output and uncertain natural gas prices. • Hybrid natural gas and renewable electricity systems can be valuable investments.

  11. Component wall thinning and a corrosion-erosion monitoring system

    International Nuclear Information System (INIS)

    Bogard, T.; Batt, T.; Roarty, D.

    1989-01-01

    Since a 1986 incident involving failure of a piping elbow due to erosion-corrosion, the electric utility industry has been actively developing technology for implementing long term programs to address corrosion-erosion. This paper describes a typical corrosion-erosion monitoring program, the types of non-destructive examinations (NDE) performed on components, and the extensive NDE data obtained when the program is applied to components in a power plant. To facilitate evaluation of the NDE data on components, an automated NDE data manipulation and data display system is advisable and perhaps necessary due to the large amounts of NDE data typically obtained during a program. Such a comprehensive corrosion-erosion monitoring system (CEMS) needs to be integral with methods for selection of inspection locations and perform NDE data analysis to help in replace, repair, or run decisions. The structure for one CEMS is described which uses IBM PC compatible hardware and a set of software addressing most data evaluation and decision making needs. CEMS features include automated input/output for typical NDE devices, database structuring, graphics outputs including color 2-D or 3-D contour plots of components, trending and predictive evaluations for future inspection planning, EC severity determination, integration of piping isometrics and component properties, and desktop publishing capabilities

  12. Plutonium Finishing Plant (PFP) HVAC System Component Index; FINAL

    International Nuclear Information System (INIS)

    DICK, J.D.

    1999-01-01

    This document identities the components, design media, procedures and defines the critical characteristics of Commercial Grade Items necessary to ensure the HVAC system provides these functions. This document lists safety class (SC) and safety significant (SS) components for the Heating Ventilation Air Conditioning (HVAC) and specifies the critical characteristics for Commercial Grade Items (CGI), as required by HNF-PRO-268 and HNF-PRO-1819. These are the minimum specifications that the equipment must meet in order to properly perform its safety function. There may be several manufacturers or models that meet the critical characteristics for any one item

  13. Qualification and issues with space flight laser systems and components

    Science.gov (United States)

    Ott, Melanie N.; Coyle, D. B.; Canham, John S.; Leidecker, Henning W., Jr.

    2006-02-01

    The art of flight quality solid-state laser development is still relatively young, and much is still unknown regarding the best procedures, components, and packaging required for achieving the maximum possible lifetime and reliability when deployed in the harsh space environment. One of the most important issues is the limited and unstable supply of quality, high power diode arrays with significant technological heritage and market lifetime. Since Spectra Diode Labs Inc. ended their involvement in the pulsed array business in the late 1990's, there has been a flurry of activity from other manufacturers, but little effort focused on flight quality production. This forces NASA, inevitably, to examine the use of commercial parts to enable space flight laser designs. System-level issues such as power cycling, operational derating, duty cycle, and contamination risks to other laser components are some of the more significant unknown, if unquantifiable, parameters that directly effect transmitter reliability. Designs and processes can be formulated for the system and the components (including thorough modeling) to mitigate risk based on the known failures modes as well as lessons learned that GSFC has collected over the past ten years of space flight operation of lasers. In addition, knowledge of the potential failure modes related to the system and the components themselves can allow the qualification testing to be done in an efficient yet, effective manner. Careful test plan development coupled with physics of failure knowledge will enable cost effect qualification of commercial technology. Presented here will be lessons learned from space flight experience, brief synopsis of known potential failure modes, mitigation techniques, and options for testing from the system level to the component level.

  14. Building 65 Hydraulic Systems Handbook: Components, Systems, and Applications

    Science.gov (United States)

    2016-04-01

    Dump Buttons OVERVIEW Pump Dump Buttons...hydraulic system? There are different types of dump buttons that control a hierarchy of flow paths. Some dump buttons are used to shut down a pump ...that branch. The use of this dump button is preferred over the Pump Dump Button when possible. Test Site Dump

  15. Skin Inqjuries Reduce Survival and Modulate Corticosterone, C-Reactive Protein, Complement Component 3, IgM, and Prostaglandin E2 after Whole-Body Reactor-Produced Mixed Field (n + γ-Photons Irradiation

    Directory of Open Access Journals (Sweden)

    Juliann G. Kiang

    2013-01-01

    Full Text Available Skin injuries such as wounds or burns following whole-body γ-irradiation (radiation combined injury (RCI increase mortality more than whole-body γ-irradiation alone. Wound-induced decreases in survival after irradiation are triggered by sustained activation of inducible nitric oxide synthase pathways, persistent alteration of cytokine homeostasis, and increased susceptibility to systemic bacterial infection. Among these factors, radiation-induced increases in interleukin-6 (IL-6 concentrations in serum were amplified by skin wound trauma. Herein, the IL-6-induced stress proteins including C-reactive protein (CRP, complement 3 (C3, immunoglobulin M (IgM, and prostaglandin E2 (PGE2 were evaluated after skin injuries given following a mixed radiation environment that might be found after a nuclear incident. In this report, mice received 3 Gy of reactor-produced mixed field (n+γ-photons radiations at 0.38 Gy/min followed by nonlethal skin wounding or burning. Both wounds and burns reduced survival and increased CRP, C3, and PGE2 in serum after radiation. Decreased IgM production along with an early rise in corticosterone followed by a subsequent decrease was noted for each RCI situation. These results suggest that RCI-induced alterations of corticosterone, CRP, C3, IgM, and PGE2 cause homeostatic imbalance and may contribute to reduced survival. Agents inhibiting these responses may prove to be therapeutic for RCI and improve related survival.

  16. Dynamic analysis of the radiolysis of binary component system

    International Nuclear Information System (INIS)

    Katayama, M.; Trumbore, C.N.

    1975-01-01

    Dynamic analysis was performed on a variety of combinations of components in the radiolysis of binary system, taking the hydrogen-producing reaction with hydrocarbon RH 2 as an example. A definite rule was able to be established from this analysis, which is useful for revealing the reaction mechanism. The combinations were as follows: 1) both components A and B do not interact but serve only as diluents, 2) A is a diluent, and B is a radical captor, 3) both A and B are radical captors, 4-1) A is a diluent, and B decomposes after the reception of the exciting energy of A, 4-2) A is a diluent, and B does not participate in decomposition after the reception of the exciting energy of A, 5-1) A is a radical captor, and B decomposes after the reception of the exciting energy of A, 5-2) A is a radical captor, and B does not participate in decomposition after the reception of the exciting energy of A, 6-1) both A and B decompose after the reception of the exciting energy of the partner component; and 6-2) both A and B do not decompose after the reception of the exciting energy of the partner component. According to the dynamical analysis of the above nine combinations, it can be pointed out that if excitation transfer participates, the similar phenomena to radical capture are presented apparently. It is desirable to measure the yield of radicals experimentally with the system which need not much consideration to the excitation transfer. Isotope substitution mixture system is conceived as one of such system. This analytical method was applied to the system containing cyclopentanone, such as cyclopentanone-cyclohexane system. (Iwakiri, K.)

  17. Passive components of NPP safety-related systems

    International Nuclear Information System (INIS)

    Ionaytis Romuald, R.; Bubnova Tatyana, A.

    2005-01-01

    This paper presents a new passive components with having drives: fast-response cutoff valves; modular actuators with opposite cocking pneumatic drives and actuation spring drives; voting electromagnetic valve units for control of pneumatic drives; passive initiators of actuation; visual diagnostics . All these devices have been developed and tested at mock-ups. This paper presents also the following direct-action passive safety components: modular pressure-relief safety valves; pilot safety valves with passive action; check valves with remote position indicator and after-tightening; modular inserts for limiting emergency coolant flow; vortex rectifier; critical weld fasteners; gas-liquid valves; fast-removable seal assembly; seal spring loaders; grooves for increasing hydraulic resistance. Replacement of active safety system components for passive ones improves the general reliability NPP by 1.5 or 2 orders of magnitudes. (authors)

  18. N-Terminal Prodomain of Pfs230 Synthesized Using a Cell-Free System Is Sufficient To Induce Complement-Dependent Malaria Transmission-Blocking Activity▿

    Science.gov (United States)

    Tachibana, Mayumi; Wu, Yimin; Iriko, Hideyuki; Muratova, Olga; MacDonald, Nicholas J.; Sattabongkot, Jetsumon; Takeo, Satoru; Otsuki, Hitoshi; Torii, Motomi; Tsuboi, Takafumi

    2011-01-01

    The aim of a malaria transmission-blocking vaccine is to block the development of malaria parasites in the mosquito and thus prevent subsequent infection of the human host. Previous studies have demonstrated that the gametocyte/gamete surface protein Pfs230 can induce transmission-blocking immunity and have evaluated Escherichia coli-produced Pfs230 as a transmission-blocking vaccine candidate. In this study, we used the wheat germ cell-free expression system to produce N-terminal fragments of Pfs230 and evaluated the transmission-blocking activity of antisera raised against the recombinant Pfs230 protein. The rabbit antisera reacted to the surface of cultured gametocytes and gametes of the Plasmodium falciparum NF54 line, recognized the 360-kDa form of parasite-produced Pfs230 by Western blot assay, and reduced the infectivity of NF54 parasites to Anopheles stefensi mosquitoes in the presence of complement in a standard membrane feeding assay. Thus, our data demonstrate that the N-terminal pro domain of Pfs230 is sufficient to induce complement-dependent transmission-blocking activity against P. falciparum. PMID:21715579

  19. N-terminal prodomain of Pfs230 synthesized using a cell-free system is sufficient to induce complement-dependent malaria transmission-blocking activity.

    Science.gov (United States)

    Tachibana, Mayumi; Wu, Yimin; Iriko, Hideyuki; Muratova, Olga; MacDonald, Nicholas J; Sattabongkot, Jetsumon; Takeo, Satoru; Otsuki, Hitoshi; Torii, Motomi; Tsuboi, Takafumi

    2011-08-01

    The aim of a malaria transmission-blocking vaccine is to block the development of malaria parasites in the mosquito and thus prevent subsequent infection of the human host. Previous studies have demonstrated that the gametocyte/gamete surface protein Pfs230 can induce transmission-blocking immunity and have evaluated Escherichia coli-produced Pfs230 as a transmission-blocking vaccine candidate. In this study, we used the wheat germ cell-free expression system to produce N-terminal fragments of Pfs230 and evaluated the transmission-blocking activity of antisera raised against the recombinant Pfs230 protein. The rabbit antisera reacted to the surface of cultured gametocytes and gametes of the Plasmodium falciparum NF54 line, recognized the 360-kDa form of parasite-produced Pfs230 by Western blot assay, and reduced the infectivity of NF54 parasites to Anopheles stefensi mosquitoes in the presence of complement in a standard membrane feeding assay. Thus, our data demonstrate that the N-terminal pro domain of Pfs230 is sufficient to induce complement-dependent transmission-blocking activity against P. falciparum.

  20. Probabilistic structural analysis methods for select space propulsion system components

    Science.gov (United States)

    Millwater, H. R.; Cruse, T. A.

    1989-01-01

    The Probabilistic Structural Analysis Methods (PSAM) project developed at the Southwest Research Institute integrates state-of-the-art structural analysis techniques with probability theory for the design and analysis of complex large-scale engineering structures. An advanced efficient software system (NESSUS) capable of performing complex probabilistic analysis has been developed. NESSUS contains a number of software components to perform probabilistic analysis of structures. These components include: an expert system, a probabilistic finite element code, a probabilistic boundary element code and a fast probability integrator. The NESSUS software system is shown. An expert system is included to capture and utilize PSAM knowledge and experience. NESSUS/EXPERT is an interactive menu-driven expert system that provides information to assist in the use of the probabilistic finite element code NESSUS/FEM and the fast probability integrator (FPI). The expert system menu structure is summarized. The NESSUS system contains a state-of-the-art nonlinear probabilistic finite element code, NESSUS/FEM, to determine the structural response and sensitivities. A broad range of analysis capabilities and an extensive element library is present.

  1. Robotic system construction with mechatronic components inverted pendulum: humanoid robot

    Science.gov (United States)

    Sandru, Lucian Alexandru; Crainic, Marius Florin; Savu, Diana; Moldovan, Cristian; Dolga, Valer; Preitl, Stefan

    2017-03-01

    Mechatronics is a new methodology used to achieve an optimal design of an electromechanical product. This methodology is collection of practices, procedures and rules used by those who work in particular branch of knowledge or discipline. Education in mechatronics at the Polytechnic University Timisoara is organized on three levels: bachelor, master and PhD studies. These activities refer and to design the mechatronics systems. In this context the design, implementation and experimental study of a family of mechatronic demonstrator occupy an important place. In this paper, a variant for a mechatronic demonstrator based on the combination of the electrical and mechanical components is proposed. The demonstrator, named humanoid robot, is equivalent with an inverted pendulum. Is presented the analyze of components for associated functions of the humanoid robot. This type of development the mechatronic systems by the combination of hardware and software, offers the opportunity to build the optimal solutions.

  2. MODELING OF SYSTEM COMPONENTS OF EDUCATIONAL PROGRAMS IN HIGH SCHOOL

    Directory of Open Access Journals (Sweden)

    E. K. Samerkhanova

    2016-01-01

    Full Text Available Based on the principles of System Studies, describes the components of the educational programs of the control system. Educational Program Management is a set of substantive, procedural, resource, subject-activity, efficiently and evaluation components, which ensures the integrity of integration processes at all levels of education. Ensuring stability and development in the management of educational programs is achieved by identifying and securing social norms, the status of the educational institution program managers to ensure the achievement of modern quality of education.Content Management provides the relevant educational content in accordance with the requirements of the educational and professional standards; process control ensures the efficient organization of rational distribution process flows; Resource Management provides optimal distribution of personnel, information and methodological, material and technical equipment of the educational program; contingent management provides subject-activity interaction of participants of the educational process; quality control ensures the quality of educational services.

  3. Secure wireless embedded systems via component-based design

    DEFF Research Database (Denmark)

    Hjorth, T.; Torbensen, R.

    2010-01-01

    This paper introduces the method secure-by-design as a way of constructing wireless embedded systems using component-based modeling frameworks. This facilitates design of secure applications through verified, reusable software. Following this method we propose a security framework with a secure c......, with full support for confidentiality, authentication, and integrity using keypairs. The approach has been demonstrated in a multi-platform home automation prototype that can remotely unlock a door using a PDA over the Internet....

  4. Component and system simulation models for High Flux Isotope Reactor

    International Nuclear Information System (INIS)

    Sozer, A.

    1989-08-01

    Component models for the High Flux Isotope Reactor (HFIR) have been developed. The models are HFIR core, heat exchangers, pressurizer pumps, circulation pumps, letdown valves, primary head tank, generic transport delay (pipes), system pressure, loop pressure-flow balance, and decay heat. The models were written in FORTRAN and can be run on different computers, including IBM PCs, as they do not use any specific simulation languages such as ACSL or CSMP. 14 refs., 13 figs

  5. Baking system for vacuum components in INDUS-2

    International Nuclear Information System (INIS)

    Bhange, Nilesh J.; Bhatnagar, Prateek; Shukla, S.K.

    2005-01-01

    Optimized bake-out procedures are very important for the overall reliability of vacuum system. In this process the UHV components like Sputter ion pump (SIP), Titanium sublimation pump (TSP) are subjected to temperature rise for sufficiently long period of time. Baking is necessary for obtaining low out gassing rates. In order to provide controlled baking of UHV (Ultra High Vacuum) components for INDUS-II an intelligent ON/OFF control system was needed. For that purpose distributed control system was suitable. For fulfillment of this need modular baking system was developed. This system contains Temperature controller unit (TCU), Pressure Monitoring Unit (PMU), and Temperature control and pressure Monitoring Interface Software (TCPMIS). Each TCU is an eight channel temperature controlling unit. PMU is eight channel pressure monitoring unit to which analog data from gauges like Penning, BA Gauge controllers is given. TCPMIS is a user interface software developed for, controlling up to 5 TCU's. In this way 40 channel temperature control, data logging of 40 channel temperature and logging of eight channel pressures was realized. The present paper describes details about computer controlled baking system. (author)

  6. Effect of component aging on PWR control rod drive systems

    International Nuclear Information System (INIS)

    Grove, E.; Gunther, W.; Sullivan, K.

    1992-01-01

    An aging assessment of PWR control rod drive (CRD) systems has been completed as part of the US NRC Nuclear Plant Aging Research (NPAR) Program. The design, construction, maintenance, and operation of the Babcock ampersand Wilcox (B ampersand W), Combustion Engineering (CE), and Westinghouse (W) systems were evaluated to determine the potential for degradation as each system ages. Operating experience data were evaluated to identify the predominant failure modes, causes, and effects. This, coupled with an assessment of the materials of construction and operating environment, demonstrate that each design is subject to degradation, which if left unchecked, could affect its safety function as the plant ages. An industry survey, conducted with the assistance of EPRI and NUMARC, identified current CRD system maintenance and inspection practices. The results of this survey indicate that some plants have performed system modifications, replaced components, or augmented existing preventive maintenance practices in response to system aging. The survey results also supported the operating experience data, which concluded that the timely replacement of degraded components, prior to failure, was not always possible using existing condition monitoring techniques. The recommendations presented in this study also include a discussion of more advanced monitoring techniques, which provide trendable results capable of detecting aging

  7. Seismic Design of ITER Component Cooling Water System-1 Piping

    Science.gov (United States)

    Singh, Aditya P.; Jadhav, Mahesh; Sharma, Lalit K.; Gupta, Dinesh K.; Patel, Nirav; Ranjan, Rakesh; Gohil, Guman; Patel, Hiren; Dangi, Jinendra; Kumar, Mohit; Kumar, A. G. A.

    2017-04-01

    The successful performance of ITER machine very much depends upon the effective removal of heat from the in-vessel components and other auxiliary systems during Tokamak operation. This objective will be accomplished by the design of an effective Cooling Water System (CWS). The optimized piping layout design is an important element in CWS design and is one of the major design challenges owing to the factors of large thermal expansion and seismic accelerations; considering safety, accessibility and maintainability aspects. An important sub-system of ITER CWS, Component Cooling Water System-1 (CCWS-1) has very large diameter of pipes up to DN1600 with many intersections to fulfill the process flow requirements of clients for heat removal. Pipe intersection is the weakest link in the layout due to high stress intensification factor. CCWS-1 piping up to secondary confinement isolation valves as well as in-between these isolation valves need to survive a Seismic Level-2 (SL-2) earthquake during the Tokamak operation period to ensure structural stability of the system in the Safe Shutdown Earthquake (SSE) event. This paper presents the design, qualification and optimization of layout of ITER CCWS-1 loop to withstand SSE event combined with sustained and thermal loads as per the load combinations defined by ITER and allowable limits as per ASME B31.3, This paper also highlights the Modal and Response Spectrum Analyses done to find out the natural frequency and system behavior during the seismic event.

  8. Lattices with unique complements

    CERN Document Server

    Saliĭ, V N

    1988-01-01

    The class of uniquely complemented lattices properly contains all Boolean lattices. However, no explicit example of a non-Boolean lattice of this class has been found. In addition, the question of whether this class contains any complete non-Boolean lattices remains unanswered. This book focuses on these classical problems of lattice theory and the various attempts to solve them. Requiring no specialized knowledge, the book is directed at researchers and students interested in general algebra and mathematical logic.

  9. Large aperture components for solid state laser fusion systems

    International Nuclear Information System (INIS)

    Simmons, W.W.

    1978-01-01

    Solid state lasers for fusion experiments must reliably deliver maximum power to small (approximately .5 mm) targets from stand-off focal distances of 1 m or more. This requirement places stringent limits upon the optical quality, resistance to damage, and overall performance of the several major components--amplifiers, Faraday isolators, spatial filters--in each amplifier train. Component development centers about achieving (1) highest functional material figure of merit, (2) best optical quality, and (3) maximum resistance to optical damage. Specific examples of the performance of large aperture components will be presented within the context of the Argus and Shiva laser systems, which are presently operational at Lawrence Livermore Laboratory. Shiva comprises twenty amplifiers, each of 20 cm output clear aperture. Terawatt beams from these amplifiers are focused through two opposed, nested clusters of f/6 lenses onto such targets. Design requirements upon the larger aperture Nova laser components, up to 35 cm in clear aperture, will also be discussed; these pose a significant challenge to the optical industry

  10. Global existence and blow-up phenomena for two-component Degasperis-Procesi system and two-component b-family system

    OpenAIRE

    Liu, Jingjing; Yin, Zhaoyang

    2014-01-01

    This paper is concerned with global existence and blow-up phenomena for two-component Degasperis-Procesi system and two-component b-family system. The strategy relies on our observation on new conservative quantities of these systems. Several new global existence results and a new blowup result of strong solutions to the two-component Degasperis- Procesi system and the two-component b-family system are presented by using these new conservative quantities.

  11. The farming system component of European agricultural landscapes

    DEFF Research Database (Denmark)

    Andersen, Erling

    2017-01-01

    Agricultural landscapes are the outcome of combined natural and human factors over time. This paper explores the scope of perceiving the agricultural landscapes of the European Union (EU) as distinct patterns of farming systems and landscape elements in homogeneous biophysical and administrative...... landscapes evolve from the praxis of the farmers and takes into account the scale, intensity and specialisation of the agricultural production. From farming system design point of view, the approach can be used to integrate the landscape in the design process. From a policy point of view, the approach offers...... endowments. The focus is on the farming systems component of the agricultural landscapes by applying a typology to the sample farms of the Farm Accountancy Data Network and scaling up the results to the landscape level for the territory of the EU. The farming system approach emphasises that agricultural...

  12. Zero-range approximation for two-component boson systems

    International Nuclear Information System (INIS)

    Sogo, T.; Fedorov, D.V.; Jensen, A.S.

    2005-01-01

    The hyperspherical adiabatic expansion method is combined with the zero-range approximation to derive angular Faddeev-like equations for two-component boson systems. The angular eigenvalues are solutions to a transcendental equation obtained as a vanishing determinant of a 3 x 3 matrix. The eigenfunctions are linear combinations of Jacobi functions of argument proportional to the distance between pairs of particles. We investigate numerically the influence of two-body correlations on the eigenvalue spectrum, the eigenfunctions and the effective hyperradial potential. Correlations decrease or increase the distance between pairs for effectively attractive or repulsive interactions, respectively. New structures appear for non-identical components. Fingerprints can be found in the nodal structure of the density distributions of the condensates. (author)

  13. Flexible Multibody Systems Models Using Composite Materials Components

    International Nuclear Information System (INIS)

    Neto, Maria Augusta; Ambr'osio, Jorge A. C.; Leal, Rog'erio Pereira

    2004-01-01

    The use of a multibody methodology to describe the large motion of complex systems that experience structural deformations enables to represent the complete system motion, the relative kinematics between the components involved, the deformation of the structural members and the inertia coupling between the large rigid body motion and the system elastodynamics. In this work, the flexible multibody dynamics formulations of complex models are extended to include elastic components made of composite materials, which may be laminated and anisotropic. The deformation of any structural member must be elastic and linear, when described in a coordinate frame fixed to one or more material points of its domain, regardless of the complexity of its geometry. To achieve the proposed flexible multibody formulation, a finite element model for each flexible body is used. For the beam composite material elements, the sections properties are found using an asymptotic procedure that involves a two-dimensional finite element analysis of their cross-section. The equations of motion of the flexible multibody system are solved using an augmented Lagrangian formulation and the accelerations and velocities are integrated in time using a multi-step multi-order integration algorithm based on the Gear method

  14. Integration Test of the High Voltage Hall Accelerator System Components

    Science.gov (United States)

    Kamhawi, Hani; Haag, Thomas; Huang, Wensheng; Pinero, Luis; Peterson, Todd; Dankanich, John

    2013-01-01

    NASA Glenn Research Center is developing a 4 kilowatt-class Hall propulsion system for implementation in NASA science missions. NASA science mission performance analysis was completed using the latest high voltage Hall accelerator (HiVHAc) and Aerojet-Rocketdyne's state-of-the-art BPT-4000 Hall thruster performance curves. Mission analysis results indicated that the HiVHAc thruster out performs the BPT-4000 thruster for all but one of the missions studied. Tests of the HiVHAc system major components were performed. Performance evaluation of the HiVHAc thruster at NASA Glenn's vacuum facility 5 indicated that thruster performance was lower than performance levels attained during tests in vacuum facility 12 due to the lower background pressures attained during vacuum facility 5 tests when compared to vacuum facility 12. Voltage-Current characterization of the HiVHAc thruster in vacuum facility 5 showed that the HiVHAc thruster can operate stably for a wide range of anode flow rates for discharge voltages between 250 and 600 volts. A Colorado Power Electronics enhanced brassboard power processing unit was tested in vacuum for 1,500 hours and the unit demonstrated discharge module efficiency of 96.3% at 3.9 kilowatts and 650 volts. Stand-alone open and closed loop tests of a VACCO TRL 6 xenon flow control module were also performed. An integrated test of the HiVHAc thruster, brassboard power processing unit, and xenon flow control module was performed and confirmed that integrated operation of the HiVHAc system major components. Future plans include continuing the maturation of the HiVHAc system major components and the performance of a single-string integration test.

  15. Sensor Failure Detection of FASSIP System using Principal Component Analysis

    Science.gov (United States)

    Sudarno; Juarsa, Mulya; Santosa, Kussigit; Deswandri; Sunaryo, Geni Rina

    2018-02-01

    In the nuclear reactor accident of Fukushima Daiichi in Japan, the damages of core and pressure vessel were caused by the failure of its active cooling system (diesel generator was inundated by tsunami). Thus researches on passive cooling system for Nuclear Power Plant are performed to improve the safety aspects of nuclear reactors. The FASSIP system (Passive System Simulation Facility) is an installation used to study the characteristics of passive cooling systems at nuclear power plants. The accuracy of sensor measurement of FASSIP system is essential, because as the basis for determining the characteristics of a passive cooling system. In this research, a sensor failure detection method for FASSIP system is developed, so the indication of sensor failures can be detected early. The method used is Principal Component Analysis (PCA) to reduce the dimension of the sensor, with the Squarred Prediction Error (SPE) and statistic Hotteling criteria for detecting sensor failure indication. The results shows that PCA method is capable to detect the occurrence of a failure at any sensor.

  16. A test system for electronics components of the PANDA MVD

    Energy Technology Data Exchange (ETDEWEB)

    Lai, Alessandra; Stockmanns, Tobias; Ritman, James [Forschungszentrum Juelich (Germany); Collaboration: PANDA-Collaboration

    2016-07-01

    The PANDA experiment is one of the main devices at the upcoming Facility for Antiproton and Ion Research(FAIR), under construction in Darmstadt, Germany. This fixed target experiment will study the transition region between perturbative and non-perturbative QCD in the energy regime of the charmonium. The innermost sub-detector system of the target spectrometer of the PANDA experiment is the Micro Vertex Detector(MVD). Two types of silicon detectors will be used: pixel detectors and double-sided strip detectors. Two front-end chips are required: the Torino Pixel ASIC(ToPix) and the PANDA Strip ASIC(PASTA). Both are designed to transmit data at a rate of several hundred Megabits per second and are capable of handling the expected hit rate in hot spots of the detector. One key component in the development of new front-end electronics is a test system capable to handle these high rates. It should be flexible enough to test different kinds of front-end electronics and it should be easy to adapt to new prototypes. Therefore, an FPGA-based system is the ideal candidate. For this test system suitable firmware and a software framework are needed. Such a system is under development at the Forschungszentrum Juelich. The main component of the Juelich Digital Readout System(JDRS) is a Virtex 6 FPGA on a development board from Xilinx. In this talk, the mentioned read-out system are introduced, and lab tests with the front-end electronics of the MVD are presented.

  17. Pathogens' toolbox to manipulate human complement.

    Science.gov (United States)

    Fernández, Francisco J; Gómez, Sara; Vega, M Cristina

    2017-12-14

    The surveillance and pathogen fighting functions of the complement system have evolved to protect mammals from life-threatening infections. In turn, pathogens have developed complex molecular mechanisms to subvert, divert and evade the effector functions of the complement. The study of complement immunoevasion by pathogens sheds light on their infection drivers, knowledge that is essential to implement therapies. At the same time, complement evasion also acts as a discovery ground that reveals important aspects of how complement works under physiological conditions. In recent years, complex interrelationships between infection insults and the onset of autoimmune and complement dysregulation diseases have led to propose that encounters with pathogens can act as triggering factors for disease. The correct management of these diseases involves the recognition of their triggering factors and the development and administration of complement-associated molecular therapies. Even more recently, unsuspected proteins from pathogens have been shown to possess moonlighting functions as virulence factors, raising the possibility that behind the first line of virulence factors there be many more pathogen proteins playing secondary, helping and supporting roles for the pathogen to successfully establish infections. In an era where antibiotics have a progressively reduced effect on the management and control of infectious diseases worldwide, knowledge on the mechanisms of pathogenic invasion and evasion look more necessary and pressing than ever. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Complement activation in leprosy: a retrospective study shows elevated circulating terminal complement complex in reactional leprosy.

    Science.gov (United States)

    Bahia El Idrissi, N; Hakobyan, S; Ramaglia, V; Geluk, A; Morgan, B Paul; Das, P Kumar; Baas, F

    2016-06-01

    Mycobacterium leprae infection gives rise to the immunologically and histopathologically classified spectrum of leprosy. At present, several tools for the stratification of patients are based on acquired immunity markers. However, the role of innate immunity, particularly the complement system, is largely unexplored. The present retrospective study was undertaken to explore whether the systemic levels of complement activation components and regulators can stratify leprosy patients, particularly in reference to the reactional state of the disease. Serum samples from two cohorts were analysed. The cohort from Bangladesh included multi-bacillary (MB) patients with (n = 12) or without (n = 46) reaction (R) at intake and endemic controls (n = 20). The cohort from Ethiopia included pauci-bacillary (PB) (n = 7) and MB (n = 23) patients without reaction and MB (n = 15) patients with reaction. The results showed that the activation products terminal complement complex (TCC) (P ≤ 0·01), C4d (P ≤ 0·05) and iC3b (P ≤ 0·05) were specifically elevated in Bangladeshi patients with reaction at intake compared to endemic controls. In addition, levels of the regulator clusterin (P ≤ 0·001 without R; P < 0·05 with R) were also elevated in MB patients, irrespective of a reaction. Similar analysis of the Ethiopian cohort confirmed that, irrespective of a reaction, serum TCC levels were increased significantly in patients with reactions compared to patients without reactions (P ≤ 0·05). Our findings suggests that serum TCC levels may prove to be a valuable tool in diagnosing patients at risk of developing reactions. © 2016 British Society for Immunology.

  19. Solar energy plant as a complement to a conventional heating system: Measurement of the storage and consumption of solar energy

    Science.gov (United States)

    Doering, E.; Lippe, W.

    1982-08-01

    The technical and economic performances of a complementary solar heating installation for a new swimming pool added to a two-floor dwelling were examined after measurements were taken over a period of 12 months and analyzed. In particular, the heat absorption and utilization were measured and modifications were carried out to improve pipe insulation and regulation of mixer valve motor running and volume flow. The collector system efficiency was evaluated at 15.4%, the proportion of solar energy of the total consumption being 6.1%. The solar plant and the measuring instruments are described and recommendations are made for improved design and performance, including enlargement of the collector surface area, further modification of the regulation system, utilization of temperature stratification in the storage tanks and avoiding mutual overshadowing of the collectors.

  20. The GNSS Component of the Seismic Monitoring System in Chile

    Science.gov (United States)

    Barrientos, S. E.

    2016-12-01

    Chile is amongst the most seismically active countries in the world. Since mid-XVI Century, a magnitude 8 or more earthquake has taken place every dozen of years, as an average. In the last 100 years, more than ten events with magnitudes around 8 or larger have taken place in this part of world. Three events with M>8 have taken place only in the last six years. The largest earthquake ever recorded took place in May, 1960, in southern Chile. Such extreme seismic activity is the result of the interaction of the Nazca, Antarctic, Scotia and South American plates in southwestern South America where Chile is located. These megathrust earthquakes exhibit long rupture regions reaching several hundreds of km with fault displacements of several tens of meters. At least eighteen of these earthquakes have generated local tsunamis with runups larger than 4 m -including events in 2010, 2014 and 2015- therefore it is mandatory to establish a system with capabilities to rapidly evaluate the tsunamigenic potential of these events. In 2013, the newly created National Seismological Center (CSN) of the University of Chile was tasked to upgrade the countrýs seismic network by increasing the numbers of real-time monitoring stations. The most important change to previous practices is the establishment of a GNSS network composed by 130 devices, in addition to the incorporation of 65 new collocated broadband and strong motion instruments. Additional 297 strong motion instruments for engineering purposes complement the system. Forty units -of the 130 devices- present an optional RTX capability, where satellite orbits and clock corrections are sent to the field device producing a 1-Hz position stream at 4-cm level. First records of ground displacement -using this technology-were recorded at the time of the largest aftershock (Mw=7.6) of the sequence that affected northern Chile in 2014. The CSN is currently developing automatic detectors and amplitude estimators of displacement from the

  1. Detecting and mitigating aging in component cooling water systems

    International Nuclear Information System (INIS)

    Lofaro, R.J.

    1991-01-01

    The time-dependent effects of aging on component cooling water (CCW) systems in nuclear power plants has been studied and documented as part of a research program sponsored by the US Nuclear Regulatory Commission. It was found that age related degradation leads to failures in the CCW system which can result in an increase in system unavailability, if not properly detected and mitigated. To identify effective methods of managing this degradation, information on inspection, monitoring, and maintenance practices currently available was obtained from various operating plants and reviewed. The findings were correlated with the most common aging mechanisms and failure modes and a compilation of aging detection and mitigation practices was formulated. This paper discusses the results of this work

  2. Detecting and mitigating aging in component cooling water systems

    International Nuclear Information System (INIS)

    Lofaro, R.J.; Aggarwal, S.

    1992-01-01

    The time-dependent effects of aging on component cooling water (CCW) systems in nuclear power plants has been studied and documented as part of a research program sponsored by the US Nuclear Regulatory Commission. It was found that age related degradation leads to failures in the CCW system which can result in an increase in system unavailability, if not properly detected and mitigated. To identify effective methods of managing this degradation, information on inspection, monitoring, and maintenance practices currently available was obtained from various operating plants and reviewed. The findings were correlated with the most common aging mechanisms and failure modes, and a compilation of aging detection and mitigation practices was formulated. This paper discusses the results of this work

  3. Does Host Complement Kill Borrelia burgdorferi within Ticks?

    OpenAIRE

    Rathinavelu, Sivaprakash; Broadwater, Anne; de Silva, Aravinda M.

    2003-01-01

    The Lyme disease spirochete, Borrelia burgdorferi, inhabits the gut lumen of the tick vector. At this location the spirochete is exposed to host blood when a tick feeds. We report here on studies that were done with normal and complement-deficient (C3-knockout) mice to determine if the host complement system killed spirochetes within the vector. We found that spirochete numbers within feeding nymphs were not influenced by complement, most likely because host complement was inactivated within ...

  4. GLACIER MONITORING SYSTEM IN COLOMBIA - complementing glaciological measurements with laser-scanning and ground-penetrating radar surveys

    Science.gov (United States)

    Ceballos, Jorge; Micheletti, Natan; Rabatel, Antoine; Mölg, Nico; Zemp, Michael

    2015-04-01

    Colombia (South America) has six small glaciers (total glacierized area of 45 Km2); their geographical location, close to zero latitude, makes them very sensitive to climate changes. An extensive monitoring program is being performed since 2006 on two glaciers, with international cooperation supports. This presentation summarizes the results of glacier changes in Colombia and includes the latest results obtained within the CATCOS Project - Phase 1 (Capacity Building and Twinning for Climate Observing Systems) signed between Colombia and Switzerland, and within the Joint Mixte Laboratory GREAT-ICE (IRD - France), with the application of LiDAR technology and GPR-based ice thickness measurements at Conejeras Glacier. Conejeras Glacier (Lat. N. 4° 48' 56"; Long. W. 75° 22' 22"; Alt. Max. 4915m.; Alt. Min. 4730m. Area 0.2 Km2) is located on the north-western side of Santa Isabel Volcano. This glacier belongs to global glacier monitoring network of the World Glacier Monitoring Service (WGMS-ID: 2721). The surface mass balance is calculated monthly using the direct glaciological method. Between April 2006 and May 2014, Conejeras Glacier showed a cumulative loss of -21 m w.e. The CATCOS Project allowed to improve the glacier monitoring system in Colombia with two main actions: (1) a terrestrial laser scanner survey (RIEGL VZ-6000 terrestrial laser scanner, property of Universities of Lausanne and Fribourg); and (2) ice thickness measurements (Blue System Integration Ltd. Ice Penetrating Radar of property of IRD). The terrestrial laser-scanning survey allowed to realize an accurate digital terrain model of the glacier surface with 13 million points and a decimetric resolution. Ice thickness measurements showed an average glacier thickness of 22 meters and a maximum of 52 meters.

  5. Chemical shift-based identification of monosaccharide spin-systems with NMR spectroscopy to complement untargeted glycomics.

    Science.gov (United States)

    Klukowski, Piotr; Schubert, Mario

    2018-06-15

    A better understanding of oligosaccharides and their wide-ranging functions in almost every aspect of biology and medicine promises to uncover hidden layers of biology and will support the development of better therapies. Elucidating the chemical structure of an unknown oligosaccharide is still a challenge. Efficient tools are required for non-targeted glycomics. Chemical shifts are a rich source of information about the topology and configuration of biomolecules, whose potential is however not fully explored for oligosaccharides. We hypothesize that the chemical shifts of each monosaccharide are unique for each saccharide type with a certain linkage pattern, so that correlated data measured by NMR spectroscopy can be used to identify the chemical nature of a carbohydrate. We present here an efficient search algorithm, GlycoNMRSearch, that matches either a subset or the entire set of chemical shifts of an unidentified monosaccharide spin system to all spin systems in an NMR database. The search output is much more precise than earlier search functions and highly similar matches suggest the chemical structure of the spin system within the oligosaccharide. Thus searching for connected chemical shift correlations within all electronically available NMR data of oligosaccharides is a very efficient way of identifying the chemical structure of unknown oligosaccharides. With an improved database in the future, GlycoNMRSearch will be even more efficient deducing chemical structures of oligosaccharides and there is a high chance that it becomes an indispensable technique for glycomics. The search algorithm presented here, together with a graphical user interface, is available at http://glyconmrsearch.santos.pwr.edu.pl. Supplementary data are available at Bioinformatics online.

  6. Application of fuzzy-MOORA method: Ranking of components for reliability estimation of component-based software systems

    Directory of Open Access Journals (Sweden)

    Zeeshan Ali Siddiqui

    2016-01-01

    Full Text Available Component-based software system (CBSS development technique is an emerging discipline that promises to take software development into a new era. As hardware systems are presently being constructed from kits of parts, software systems may also be assembled from components. It is more reliable to reuse software than to create. It is the glue code and individual components reliability that contribute to the reliability of the overall system. Every component contributes to overall system reliability according to the number of times it is being used, some components are of critical usage, known as usage frequency of component. The usage frequency decides the weight of each component. According to their weights, each component contributes to the overall reliability of the system. Therefore, ranking of components may be obtained by analyzing their reliability impacts on overall application. In this paper, we propose the application of fuzzy multi-objective optimization on the basis of ratio analysis, Fuzzy-MOORA. The method helps us find the best suitable alternative, software component, from a set of available feasible alternatives named software components. It is an accurate and easy to understand tool for solving multi-criteria decision making problems that have imprecise and vague evaluation data. By the use of ratio analysis, the proposed method determines the most suitable alternative among all possible alternatives, and dimensionless measurement will realize the job of ranking of components for estimating CBSS reliability in a non-subjective way. Finally, three case studies are shown to illustrate the use of the proposed technique.

  7. A two component system is involved in acid adaptation of Lactobacillus delbrueckii subsp. bulgaricus.

    Science.gov (United States)

    Cui, Yanhua; Liu, Wei; Qu, Xiaojun; Chen, Zhangting; Zhang, Xu; Liu, Tong; Zhang, Lanwei

    2012-05-20

    The Gram-positive bacterium Lactobacillus delbrueckii subsp. bulgaricus is of vital importance to the food industry, especially to the dairy industry. Two component systems (TCSs) are one of the most important mechanisms for environmental sensing and signal transduction in the majority of Gram-positive and Gram-negative bacteria. A typical TCS consists of a histidine protein kinase (HPK) and a cytoplasmic response regulator (RR). To investigate the functions of TCSs during acid adaptation in L. bulgaricus, we used quantitative PCR to reveal how TCSs expression changes during acid adaptation. Two TCSs (JN675228/JN675229 and JN675230/JN675231) and two HPKs (JN675236 and JN675240) were induced during acid adaptation. These TCSs were speculated to be related with the acid adaptation ability of L. bulgaricus. The mutants of JN675228/JN675229 were constructed in order to investigate the functions of JN675228/JN675229. The mutants showed reduced acid adaptation compared to that of wild type, and the complemented strains were similar to the wild-type strain. These observations suggested that JN675228 and JN675229 were involved in acid adaptation in L. bulgaricus. The interaction between JN675228 and JN675229 was identified by means of yeast two-hybrid system. The results indicated there is interaction between JN675228 and JN675229. Crown Copyright © 2011. Published by Elsevier GmbH. All rights reserved.

  8. The Evolution of Two-Component Systems in Bacteria RevealsDifferent Strategies for Niche Adaptation

    Energy Technology Data Exchange (ETDEWEB)

    Alm, Eric; Huang, Katherine; Arkin, Adam

    2006-09-13

    Two-component systems including histidine protein kinasesrepresent the primary signal transduction paradigm in prokaryoticorganisms. To understand how these systems adapt to allow organisms todetect niche-specific signals, we analyzed the phylogenetic distributionof nearly 5000 histidine protein kinases from 207 sequenced prokaryoticgenomes. We found that many genomes carry a large repertoire of recentlyevolved signaling genes, which may reflect selective pressure to adapt tonew environmental conditions. Both lineage-specific gene family expansionand horizontal gene transfer play major roles in the introduction of newhistidine kinases into genomes; however, there are differences in howthese two evolutionary forces act. Genes imported via horizontal transferare more likely to retain their original functionality as inferred from asimilar complement of signaling domains, while gene family expansionaccompanied by domain shuffling appears to be a major source of novelgenetic diversity. Family expansion is the dominantsource of newhistidine kinase genes in the genomes most enriched in signalingproteins, and detailed analysis reveals that divergence in domainstructure and changes in expression patterns are hallmarks of recentexpansions. Finally, while these two modes of gene acquisition arewidespread across bacterial taxa, there are clear species-specificpreferences for which mode is used.

  9. Reduction in erythrocyte-bound complement activation products and titres of anti-C1q antibodies associate with clinical improvement in systemic lupus erythematosus.

    Science.gov (United States)

    Buyon, Jill; Furie, Richard; Putterman, Chaim; Ramsey-Goldman, Rosalind; Kalunian, Kenneth; Barken, Derren; Conklin, John; Dervieux, Thierry

    2016-01-01

    The relationship between cell-bound complement activation products (CB-CAPs: EC4d, EC3d), anti-C1q, soluble complement C3/C4 and disease activity in systemic lupus erythematosus (SLE) was evaluated. Per protocol, at baseline all SLE subjects enrolled in this longitudinal study presented with active disease and elevated CB-CAPs. At each monthly visit, the non-serological (ns) Safety of Estrogens in Lupus Erythematosus: National Assessment (SELENA-SLEDAI) and the British Isles Lupus Assessment Group (BILAG)-2004 index scores were determined as was a random urinary protein to creatinine ratio (uPCR). Short-form 36 (SF-36) questionnaires were also collected. All soluble markers were determined using immunoassays, while EC4d and EC3d were determined using flow cytometry. Statistical analysis consisted of linear mixed models with random intercept and fixed slopes. A total of 36 SLE subjects (mean age 34 years; 94% female) were enrolled and evaluated monthly for an average 11 visits per subject. Clinical improvements were observed during the study, with significant decreases in ns-SELENA-SLEDAI scores, BILAG-2004 index scores and uPCR, and increases in all domains of SF-36 (pimprovements in at least six out of the eight domains of SF-36 and outperformed C3/C4. Anti-dsDNA titres did not correlate with changes in disease activity. These data indicate that CB-CAPs and anti-C1q are helpful in monitoring patients with SLE.

  10. An Efficient Connected Component Labeling Architecture for Embedded Systems

    Directory of Open Access Journals (Sweden)

    Fanny Spagnolo

    2018-03-01

    Full Text Available Connected component analysis is one of the most fundamental steps used in several image processing systems. This technique allows for distinguishing and detecting different objects in images by assigning a unique label to all pixels that refer to the same object. Most of the previous published algorithms have been designed for implementation by software. However, due to the large number of memory accesses and compare, lookup, and control operations when executed on a general-purpose processor, they do not satisfy the speed performance required by the next generation high performance computer vision systems. In this paper, we present the design of a new Connected Component Labeling hardware architecture suitable for high performance heterogeneous image processing of embedded designs. When implemented on a Zynq All Programmable-System on Chip (AP-SOC 7045 chip, the proposed design allows a throughput rate higher of 220 Mpixels/s to be reached using less than 18,000 LUTs and 5000 FFs, dissipating about 620 μJ.

  11. Aging of systems: theoretical investigations on system and components time behaviour

    International Nuclear Information System (INIS)

    Eid, M.; Coudray, R.

    1995-01-01

    Being a direct indicator of aging, the systems time-dependent failure rates need to be evaluated using qualified methodologies and starting from basic components time-dependent failure data. Basic component time-dependent failure data are not often available. Components failure data used in the paper are issued from some theoretical considerations rather than from field statistical observations. Four academic cases are presented and their results are discussed. Evaluations result in, very often, systems time-dependent failure rates that require understanding and careful interpretation. Kinetic trends of systems and of components may sometimes be different. (authors). 4 figs., 3 tabs., 3 refs., 1 appendix

  12. Material properties in complement activation

    DEFF Research Database (Denmark)

    Moghimi, S. Moein; Andersen, Alina Joukainen; Ahmadvand, Davoud

    2011-01-01

    activation differently and through different sensing molecules and initiation pathways. The importance of material properties in triggering complement is considered and mechanistic aspects discussed. Mechanistic understanding of complement events could provide rational approaches for improved material design...

  13. Optical Components for Reconfigurable Photonic Networks and Mobile Systems

    DEFF Research Database (Denmark)

    Vegas Olmos, Juan José; Rodríguez Páez, Juan Sebastián; Rommel, Simon

    communication needs to move to the millimeter-wavelength or sub-terahertz range. Radio-over-Fiber (RoF) technologies have evolved from a blue sky academic topic in the 90s to a main driver within the current quest for the 5th generation mobile systems (5G). A twist in RoF technologies is that it has found along...... processing, high speed modulators and photodiodes and optical switching technologies. A deep overview on the state-of-the-art and current efforts towards optical components enabling photonic reconfigurability will be given during the presentation....

  14. Effect of component design in retrieved bipolar hip hemiarthroplasty systems.

    Science.gov (United States)

    Hess, Matthew D; Baker, Erin A; Salisbury, Meagan R; Kaplan, Lige M; Greene, Ryan T; Greene, Perry W

    2013-09-01

    Primary articulation of bipolar hemiarthroplasty systems is at the femoral head-liner interface. The purpose of this study was to compare observed damage modes on 36 retrieved bipolar systems with implant, demographic, intraoperative, and radiographic data to elucidate the effects of component design, specifically locking mechanism, on clinical performance. Retrieved bipolar hip hemiarthroplasty systems of 3 different design types were obtained, disassembled, and evaluated macro- and microscopically for varying modes of wear, including abrasion, burnishing, embedding, scratching, and pitting. Clinical record review and radiographic analysis were performed by a senior orthopedic surgery resident. Average bipolar hip hemiarthroplasty system term of service was 46 months (range, 0.27-187 months). All devices contained wear debris captured within the articulating space between the femoral head and liner. In 31% of patients without infection, lucency was observed on immediate prerevision radiographs. The system with a leaf locking mechanism showed significantly increased radiographically observed osteolysis (P=.03) compared with a system with a stopper ring locking mechanism. In addition, implant design and observed damage modes, including pitting and third-body particle embedding, were significantly associated with radiographically observed osteolysis. Copyright 2013, SLACK Incorporated.

  15. An efficient rHSV-based complementation system for the production of multiple rAAV vector serotypes.

    Science.gov (United States)

    Kang, W; Wang, L; Harrell, H; Liu, J; Thomas, D L; Mayfield, T L; Scotti, M M; Ye, G J; Veres, G; Knop, D R

    2009-02-01

    Recombinant herpes simplex virus type 1 (rHSV)-assisted recombinant adeno-associated virus (rAAV) vector production provides a highly efficient and scalable method for manufacture of clinical grade rAAV vectors. Here, we present an rHSV co-infection system for rAAV production, which uses two ICP27-deficient rHSV constructs, one bearing the rep2 and cap (1, 2 or 9) genes of rAAV, and the second bearing an AAV2 ITR-gene of interest (GOI) cassette. The optimum rAAV production parameters were defined by producing rAAV2/GFP in HEK293 cells, yielding greater than 9000 infectious particles per cell with a 14:1 DNase resistance particle to infectious particle (DRP/ip) ratio. The optimized co-infection parameters were then used to generate large-scale stocks of rAAV1/AAT, which encode the human alpha-1-antitrypsin (hAAT) protein, and purified by column chromatography. The purified vector was extensively characterized by rAAV- and rHSV-specific assays and compared to transfection-made vector for in vivo efficacy in mice through intramuscular injection. The co-infection method was also used to produce rAAV9/AAT for comparison to rAAV1/AAT in vivo. Intramuscular administration of 1 x 10(11) DRP per animal of rHSV-produced rAAV1/AAT and rAAV9/AAT resulted in hAAT protein expression of 5.4 x 10(4) and 9.4 x 10(5) ng ml(-1) serum respectively, the latter being clinically relevant.

  16. Autocrine Effects of Tumor-Derived Complement

    Directory of Open Access Journals (Sweden)

    Min Soon Cho

    2014-03-01

    Full Text Available We describe a role for the complement system in enhancing cancer growth. Cancer cells secrete complement proteins that stimulate tumor growth upon activation. Complement promotes tumor growth via a direct autocrine effect that is partially independent of tumor-infiltrating cytotoxic T cells. Activated C5aR and C3aR signal through the PI3K/AKT pathway in cancer cells, and silencing the PI3K or AKT gene in cancer cells eliminates the progrowth effects of C5aR and C3aR stimulation. In patients with ovarian or lung cancer, higher tumoral C3 or C5aR mRNA levels were associated with decreased overall survival. These data identify a role for tumor-derived complement proteins in promoting tumor growth, and they therefore have substantial clinical and therapeutic implications.

  17. Major Components of the National TRU Waste System Optimization Project

    International Nuclear Information System (INIS)

    Moody, D.C.; Bennington, B.; Sharif, F.

    2002-01-01

    The National Transuranic (TRU) Program (NTP) is being optimized to allow for disposing of the legacy TRU waste at least 10 years earlier than originally planned. This acceleration will save the nation an estimated $713. The Department of Energy's (DOE'S) Carlsbad Field Office (CBFO) has initiated the National TRU Waste System Optimization Project to propose, and upon approvaI, implement activities that produce significant cost saving by improving efficiency, thereby accelerating the rate of TRU waste disposal without compromising safety. In its role as NTP agent of change, the National TRU Waste System Optimization Project (the Project) (1) interacts closely with all NTP activities. Three of the major components of the Project are the Central Characterization Project (CCP), the Central Confirmation Facility (CCF), and the MobiIe/Modular Deployment Program.

  18. System and method for manufacture of airfoil components

    Science.gov (United States)

    Moors, Thomas Michael

    2016-11-29

    Embodiments of the present disclosure relate generally to systems and methods for manufacturing an airfoil component. The system can include: a geometrical mold; an elongated flexible sleeve having a closed-off interior and positioned within the geometrical mold, wherein the elongated flexible sleeve is further positioned to have a desired geometry; an infusing channel in fluid communication with the closed-off interior of the elongated flexible sleeve and configured to communicate a resinous material thereto; a vacuum channel in fluid communication with the closed-off interior of the elongated flexible sleeve and configured to vacuum seal the closed-off interior of the elongated flexible sleeve; and a glass fiber layer positioned within the closed-off interior of the elongated flexible sleeve.

  19. Development of expert system for structural design of FBR components

    International Nuclear Information System (INIS)

    Ueda, Hiroyoshi; Uno, Masayoshi; Ogawa, Hiroshi; Shimakawa, Takashi; Yoshimura, Shinobu; Yagawa, Genki.

    1995-01-01

    The characteristics of structural design processes for nuclear components can be summarized as follows : (1) Many engineers belonging to different fields are working in parallel, exchanging a huge amount of data and information. (2) A final solution is determined after a number of iterative design processes. (3) Solutions have to be examined many times based on sophisticated design codes. (4) Sophisticated calculation methods such as the finite element method are frequently utilized, and experts' knowledge on such analyses plays important roles in the design process. Taking these issues into consideration, a new expert system for structural design is developed in the present study. Here, the object-oriented data flow mechanism and the blackboard model are utilized to systematize structural design processes in a computer. An automated finite element calculation module is implemented, and experts' knowledge is stored in knowledge base. In addition, a new algorithm is employed to automatically draw the design window, which is defined as an area of permissible solutions in a design parameter space. The developed system is successfully applied to obtain the design windows of four components selected from the demonstration FBR structures. (author)

  20. Food-grade host/vector expression system for Lactobacillus casei based on complementation of plasmid-associated phospho-beta-galactosidase gene lacG.

    Science.gov (United States)

    Takala, T M; Saris, P E J; Tynkkynen, S S H

    2003-01-01

    A new food-grade host/vector system for Lactobacillus casei based on lactose selection was constructed. The wild-type non-starter host Lb. casei strain E utilizes lactose via a plasmid-encoded phosphotransferase system. For food-grade cloning, a stable lactose-deficient mutant was constructed by deleting a 141-bp fragment from the phospho-beta-galactosidase gene lacG via gene replacement. The deletion resulted in an inactive phospho-beta-galactosidase enzyme with an internal in-frame deletion of 47 amino acids. A complementation plasmid was constructed containing a replicon from Lactococcus lactis, the lacG gene from Lb. casei, and the constitutive promoter of pepR for lacG expression from Lb. rhamnosus. The expression of the lacG gene from the resulting food-grade plasmid pLEB600 restored the ability of the lactose-negative mutant strain to grow on lactose to the wild-type level. The vector pLEB600 was used for expression of the proline iminopeptidase gene pepI from Lb. helveticus in Lb. casei. The results show that the food-grade expression system reported in this paper can be used for expression of foreign genes in Lb. casei.

  1. Computer software program for monitoring the availability of systems and components of electric power generating systems

    International Nuclear Information System (INIS)

    Petersen, T.A.; Hilsmeier, T.A.; Kapinus, D.M.

    1994-01-01

    As availabilities of electric power generating stations systems and components become more and more important from a financial, personnel safety, and regulatory requirements standpoint, it is evident that a comprehensive, yet simple and user-friendly program for system and component tracking and monitoring is needed to assist in effectively managing the large volume of systems and components with their large numbers of associated maintenance/availability records. A user-friendly computer software program for system and component availability monitoring has been developed that calculates, displays and monitors selected component and system availabilities. This is a Windows trademark based (Graphical User Interface) program that utilizes a system flow diagram for the data input screen which also provides a visual representation of availability values and limits for the individual components and associated systems. This program can be customized to the user's plant-specific system and component selections and configurations. As will be discussed herein, this software program is well suited for availability monitoring and ultimately providing valuable information for improving plant performance and reducing operating costs

  2. Economic Optimization of Component Sizing for Residential Battery Storage Systems

    Directory of Open Access Journals (Sweden)

    Holger C. Hesse

    2017-06-01

    Full Text Available Battery energy storage systems (BESS coupled with rooftop-mounted residential photovoltaic (PV generation, designated as PV-BESS, draw increasing attention and market penetration as more and more such systems become available. The manifold BESS deployed to date rely on a variety of different battery technologies, show a great variation of battery size, and power electronics dimensioning. However, given today’s high investment costs of BESS, a well-matched design and adequate sizing of the storage systems are prerequisites to allow profitability for the end-user. The economic viability of a PV-BESS depends also on the battery operation, storage technology, and aging of the system. In this paper, a general method for comprehensive PV-BESS techno-economic analysis and optimization is presented and applied to the state-of-art PV-BESS to determine its optimal parameters. Using a linear optimization method, a cost-optimal sizing of the battery and power electronics is derived based on solar energy availability and local demand. At the same time, the power flow optimization reveals the best storage operation patterns considering a trade-off between energy purchase, feed-in remuneration, and battery aging. Using up to date technology-specific aging information and the investment cost of battery and inverter systems, three mature battery chemistries are compared; a lead-acid (PbA system and two lithium-ion systems, one with lithium-iron-phosphate (LFP and another with lithium-nickel-manganese-cobalt (NMC cathode. The results show that different storage technology and component sizing provide the best economic performances, depending on the scenario of load demand and PV generation.

  3. Investigation on Prefabricated Building System Skilled Component Installers

    Directory of Open Access Journals (Sweden)

    Nurul Rezuana Buyung

    2013-09-01

    Full Text Available In the face of an increasingly challenging era of globalization, skills and new equipments which includes prefabricated building components, known as Industrialized Building System (IBS has been introduced towards achievement of sustainable construction. IBS is a construction system in which the components are manufactured in a factory, on or off site, positioned and assembled into complete structures with minimal additional site work. IBS requires high construction precision and needs a higher skill level of workers. Compared to the conventional construction method, the skill level of IBS workers is more demanding. Although there are a lot benefits in implementing IBS, the construction industry still not rapidly implementing IBS. The IBS method still considered new and even though there are a lot of benefits it still faces barriers. In an IBS construction, the role of the contractor is shifted from a builder to an assembler on the site. Therefore, this requires the contractor to be prepared technologically with IBS knowledge and skills. It is generally perceived that the number of skilled IBS installers in Malaysia is still low even though the system has been implemented for a long time. This research is carried out to find out whether the existing number of IBS installer is sufficient. Primary data was collected by carrying out interviews with the contractors at the IBS construction site in Penang Island in order to get the contractor’s feedback regarding this issue. Meanwhile, the secondary data was collected from government agencies to get the number of existing IBS installer and the number of IBS projects done in government projects. The results from this study indicated that not all categories of skill workers are in shortage. However, the number of precast concrete installer is in a critical shortage.

  4. Component-Level Electronic-Assembly Repair (CLEAR) System Architecture

    Science.gov (United States)

    Oeftering, Richard C.; Bradish, Martin A.; Juergens, Jeffrey R.; Lewis, Michael J.; Vrnak, Daniel R.

    2011-01-01

    This document captures the system architecture for a Component-Level Electronic-Assembly Repair (CLEAR) capability needed for electronics maintenance and repair of the Constellation Program (CxP). CLEAR is intended to improve flight system supportability and reduce the mass of spares required to maintain the electronics of human rated spacecraft on long duration missions. By necessity it allows the crew to make repairs that would otherwise be performed by Earth based repair depots. Because of practical knowledge and skill limitations of small spaceflight crews they must be augmented by Earth based support crews and automated repair equipment. This system architecture covers the complete system from ground-user to flight hardware and flight crew and defines an Earth segment and a Space segment. The Earth Segment involves database management, operational planning, and remote equipment programming and validation processes. The Space Segment involves the automated diagnostic, test and repair equipment required for a complete repair process. This document defines three major subsystems including, tele-operations that links the flight hardware to ground support, highly reconfigurable diagnostics and test instruments, and a CLEAR Repair Apparatus that automates the physical repair process.

  5. Complementing Gender Analysis Methods.

    Science.gov (United States)

    Kumar, Anant

    2016-01-01

    The existing gender analysis frameworks start with a premise that men and women are equal and should be treated equally. These frameworks give emphasis on equal distribution of resources between men and women and believe that this will bring equality which is not always true. Despite equal distribution of resources, women tend to suffer and experience discrimination in many areas of their lives such as the power to control resources within social relationships, and the need for emotional security and reproductive rights within interpersonal relationships. These frameworks believe that patriarchy as an institution plays an important role in women's oppression, exploitation, and it is a barrier in their empowerment and rights. Thus, some think that by ensuring equal distribution of resources and empowering women economically, institutions like patriarchy can be challenged. These frameworks are based on proposed equality principle which puts men and women in competing roles. Thus, the real equality will never be achieved. Contrary to the existing gender analysis frameworks, the Complementing Gender Analysis framework proposed by the author provides a new approach toward gender analysis which not only recognizes the role of economic empowerment and equal distribution of resources but suggests to incorporate the concept and role of social capital, equity, and doing gender in gender analysis which is based on perceived equity principle, putting men and women in complementing roles that may lead to equality. In this article the author reviews the mainstream gender theories in development from the viewpoint of the complementary roles of gender. This alternative view is argued based on existing literature and an anecdote of observations made by the author. While criticizing the equality theory, the author offers equity theory in resolving the gender conflict by using the concept of social and psychological capital.

  6. Reliability Prediction Of System And Component Of Process System Of RSG-GAS Reactor

    International Nuclear Information System (INIS)

    Sitorus Pane, Jupiter

    2001-01-01

    The older the reactor the higher the probability of the system and components suffer from loss of function or degradation. This phenomenon occurred because of wear, corrosion, and fatigue. Study on component reliability was generally performed deterministically and statistically. This paper would describe an analysis of using statistical method, i.e. regression Cox, in order to predict the reliability of the components and their environmental influence's factors. The result showed that the dynamics, non safety related, and mechanic components have higher risk of failure, whereas static, safety related, and electric have lower risk of failures. The relative risk value for variable of components dynamics, quality, dummy 1 and dummy 2 are of 1.54, 1.59, 1.50, and 0.83 compare to other components type with each variable. Component with the higher risk have lower reliability than lower one

  7. Modular Engine Noise Component Prediction System (MCP) Program Users' Guide

    Science.gov (United States)

    Golub, Robert A. (Technical Monitor); Herkes, William H.; Reed, David H.

    2004-01-01

    This is a user's manual for Modular Engine Noise Component Prediction System (MCP). This computer code allows the user to predict turbofan engine noise estimates. The program is based on an empirical procedure that has evolved over many years at The Boeing Company. The data used to develop the procedure include both full-scale engine data and small-scale model data, and include testing done by Boeing, by the engine manufacturers, and by NASA. In order to generate a noise estimate, the user specifies the appropriate engine properties (including both geometry and performance parameters), the microphone locations, the atmospheric conditions, and certain data processing options. The version of the program described here allows the user to predict three components: inlet-radiated fan noise, aft-radiated fan noise, and jet noise. MCP predicts one-third octave band noise levels over the frequency range of 50 to 10,000 Hertz. It also calculates overall sound pressure levels and certain subjective noise metrics (e.g., perceived noise levels).

  8. System, structure, and component evaluation for life-cycle management

    International Nuclear Information System (INIS)

    Hanley, N.E.; Banerjee, A.K.; Woods, P.B.; Perrin, J.S.; Marian, F.A.

    1992-01-01

    In recent years, many nuclear organizations and utilities have studied the possibility of extending the service life of nuclear power plants beyond the original license period. From these studies, recommendations have resulted for maintaining the option of future decisions concerning operating license renewal. Several of the recommendations are considered beneficial to the management and operation of nuclear plants in meeting many of their near-term goals. In 1986, Public Service Electric and Gas (PSE and G) concluded that a full-scale nuclear plant license renewal program for their Salem 1 and 2 and Hope Creek nuclear stations was not cost-effective at that time. Rather, it would be better served if the nuclear plant life extension (PLEX) option were maintained for future consideration. To help plan for the life extension option, a strategic 5-yr life cycle management (LCM) program was begun. In support of the LCM program, evaluations for the following Salem structures and components were performed: (1) intake structures, (2) reactor vessel support, (3) containment liner, and (4) containment structure (below grade). This paper discusses the systems, structures, and components (SSC) evaluation methodology and, as an example, discusses the evaluation performed for reactor vessel support

  9. Calorimetric Measuring Systems for Characterizing High Frequency Power Losses in Power Electronic Components and Systems

    DEFF Research Database (Denmark)

    Blaabjerg, Frede; Pedersen, John Kim; Ritchie, Andrew Ewen

    2002-01-01

    High frequency power losses in power electronic components and systems are very difficult to measure. The same applies to the efficiency of high-efficiency systems and components. An important method to measure losses with high accuracy is the calorimetric measuring systems. This paper describes...... to calibrate such systems are proposed and different applications of the system are given. Two practical examples end the description of the research. It is concluded that such systems have a relative long time-constant but they are accurate and useful for precise power loss measurement....

  10. Aging techniques and qualified life for safety system components

    International Nuclear Information System (INIS)

    Weaver, W.W.

    1980-01-01

    Presently, the qualified life objective for Class IE safety system components in nuclear power plants is somewhat of a subjective engineering judgment. When the desired qualified life is ascertained, there are other choices that must be made (which may be influenced by the desired qualified life) such as selecting the aging procedure to use in the qualification process. Adding complexity to the situation is the fact that there are some limitations in aging techniques at the present time. This article presents (1) a discussion of the limitations in aging procedures, (2) the general philosophy of qualification, and (3) a proposed method for specifying a desired qualified life, which uses a probabilistic approach. The probabilistic approach proposed in item 3 can be applied to natural aging programs and eventually to accelerated aging once the present technical difficulties are overcome

  11. Critical mm-wave components for synthetic automatic test systems

    CERN Document Server

    Hrobak, Michael

    2015-01-01

    Michael Hrobak studied hybrid integrated front end modules for high frequency measurement equipment and especially for synthetic automatic test systems. Recent developments of innovative, critical millimeter-wave components like frequency multipliers, directional couplers, filters, triple balanced mixers and power detectors are illustrated by the author separately and in combination.  Contents Synthetic Instruments Resistive Diode Frequency Multipliers Planar Directional Couplers and Filters Triple Balanced Mixers Zero Bias Schottky Power Detectors Integrated Front End Assemblies  Target Groups Scientists and students in the field of electrical engineering with main emphasis on high frequency technology Engineers and Practitioners dealing with the development of micro- and millimeter-wave measurement instruments  About the Author Dr. Michael Hrobak is with the Microwave Department of the Ferdinand-Braun-Institut (FBH), Berlin, Germany, where he is involved in the development and measurement of monolithic i...

  12. Phosphatase activity tunes two-component system sensor detection threshold.

    Science.gov (United States)

    Landry, Brian P; Palanki, Rohan; Dyulgyarov, Nikola; Hartsough, Lucas A; Tabor, Jeffrey J

    2018-04-12

    Two-component systems (TCSs) are the largest family of multi-step signal transduction pathways in biology, and a major source of sensors for biotechnology. However, the input concentrations to which biosensors respond are often mismatched with application requirements. Here, we utilize a mathematical model to show that TCS detection thresholds increase with the phosphatase activity of the sensor histidine kinase. We experimentally validate this result in engineered Bacillus subtilis nitrate and E. coli aspartate TCS sensors by tuning their detection threshold up to two orders of magnitude. We go on to apply our TCS tuning method to recently described tetrathionate and thiosulfate sensors by mutating a widely conserved residue previously shown to impact phosphatase activity. Finally, we apply TCS tuning to engineer B. subtilis to sense and report a wide range of fertilizer concentrations in soil. This work will enable the engineering of tailor-made biosensors for diverse synthetic biology applications.

  13. Noise of screen-film systems: origins and components

    Energy Technology Data Exchange (ETDEWEB)

    Kuhn, H

    1983-01-01

    When using the more sensitive rare-earth intensifying screens in radiography applying the screen-film system, one has to cope with an increase in quantum noise. Measurement of Wiener spectra will help to determine the noise of the film. With an appropriate apparatus, the noise spectra of screen-film systems of different sensitivity have been ascertained and compared with theoretical assessments. The integral noise made up of the components film noise, screen noise and quantum noise have been thoroughly analysed. Adequate choice of radiographic conditions (such as modification of film exposure time via the screen, change of tube voltage) will affect the number of absorbed X-ray quanta in the luminous substance and thus the quantum noise which, as was found out, largely contributes to the integral noise together with another factor, graininess of the film. The study shows that although quantum noise has to be cut back, this should not be done at any price, and due regard must be paid to other factors influencing the image quality of the system, such as contrast and MTF.

  14. Solid Propulsion Systems, Subsystems, and Components Service Life Extension

    Science.gov (United States)

    Hundley, Nedra H.; Jones, Connor

    2011-01-01

    The service life extension of solid propulsion systems, subsystems, and components will be discussed based on the service life extension of the Space Transportation System Reusable Solid Rocket Motor (RSRM) and Booster Separation Motors (BSM). The RSRM is certified for an age life of five years. In the aftermath of the Columbia accident there were a number of motors that were approaching the end of their five year service life certification. The RSRM Project initiated an assessment to determine if the service life of these motors could be extended. With the advent of the Constellation Program, a flight test was proposed that would utilize one of the RSRMs which had been returned from the launch site due to the expiration of its five year service life certification and twelve surplus Chemical Systems Division BSMs which had exceeded their eight year service life. The RSRM age life tracking philosophy which establishes when the clock starts for age life tracking will be described. The role of the following activities in service life extension will be discussed: subscale testing, accelerated aging, dissecting full scale aged hardware, static testing full scale aged motors, data mining industry data, and using the fleet leader approach. The service life certification and extension of the BSMs will also be presented.

  15. Modeling and validation of existing VAV system components

    Energy Technology Data Exchange (ETDEWEB)

    Nassif, N.; Kajl, S.; Sabourin, R. [Ecole de Technologie Superieure, Montreal, PQ (Canada)

    2004-07-01

    The optimization of supervisory control strategies and local-loop controllers can improve the performance of HVAC (heating, ventilating, air-conditioning) systems. In this study, the component model of the fan, the damper and the cooling coil were developed and validated against monitored data of an existing variable air volume (VAV) system installed at Montreal's Ecole de Technologie Superieure. The measured variables that influence energy use in individual HVAC models included: (1) outdoor and return air temperature and relative humidity, (2) supply air and water temperatures, (3) zone airflow rates, (4) supply duct, outlet fan, mixing plenum static pressures, (5) fan speed, and (6) minimum and principal damper and cooling and heating coil valve positions. The additional variables that were considered, but not measured were: (1) fan and outdoor airflow rate, (2) inlet and outlet cooling coil relative humidity, and (3) liquid flow rate through the heating or cooling coils. The paper demonstrates the challenges of the validation process when monitored data of existing VAV systems are used. 7 refs., 11 figs.

  16. Hijacking Complement Regulatory Proteins for Bacterial Immune Evasion.

    Science.gov (United States)

    Hovingh, Elise S; van den Broek, Bryan; Jongerius, Ilse

    2016-01-01

    The human complement system plays an important role in the defense against invading pathogens, inflammation and homeostasis. Invading microbes, such as bacteria, directly activate the complement system resulting in the formation of chemoattractants and in effective labeling of the bacteria for phagocytosis. In addition, formation of the membrane attack complex is responsible for direct killing of Gram-negative bacteria. In turn, bacteria have evolved several ways to evade complement activation on their surface in order to be able to colonize and invade the human host. One important mechanism of bacterial escape is attraction of complement regulatory proteins to the microbial surface. These molecules are present in the human body for tight regulation of the complement system to prevent damage to host self-surfaces. Therefore, recruitment of complement regulatory proteins to the bacterial surface results in decreased complement activation on the microbial surface which favors bacterial survival. This review will discuss recent advances in understanding the binding of complement regulatory proteins to the bacterial surface at the molecular level. This includes, new insights that have become available concerning specific conserved motives on complement regulatory proteins that are favorable for microbial binding. Finally, complement evasion molecules are of high importance for vaccine development due to their dominant role in bacterial survival, high immunogenicity and homology as well as their presence on the bacterial surface. Here, the use of complement evasion molecules for vaccine development will be discussed.

  17. Does autonomization of public hospitals and exposure to market pressure complement or debilitate social health insurance systems? Evidence from a low-income country.

    Science.gov (United States)

    Sepehri, Ardeshir

    2014-01-01

    Granting public hospitals greater autonomy and creating organizational arrangements that mimic the private sector and encourage competition is often promoted as a way to increase efficiency and public accountability and to improve quality of care at these facilities. The existence of good-quality health infrastructure, in turn, encourages the population to join and support the social health insurance system and achieve universal coverage. This article provides a critical review of hospital autonomization, using Vietnam's experience to assess the influence of hospital autonomy on the sustainability of Vietnam's social health insurance. The evidence suggests that a reform process based on greater autonomy of resource mobilization and on the retention and use of own-source revenues can create perverse incentives among managers and health care providers, leading to the development of a two-tiered provision of clinical care, provider-induced supply of an inefficient service mix, a high degree of duplication, wasteful investment, and cost escalation. Rather than complementing social health insurance and helping the country to achieve universal coverage, granting public hospitals greater autonomy that mimics the private sector may indeed undermine the legitimacy and sustainability of social health insurance as health care costs escalate and higher quality of care remains elusive.

  18. The condition monitoring system of turbine system components for nuclear power plants

    International Nuclear Information System (INIS)

    Ono, Shigetoshi

    2013-01-01

    The thermal and nuclear power plants have been imposed a stable supply of electricity. To certainly achieve this, we built the plant condition monitoring system based on the heat and mass balance calculation. If there are some performance changes on the turbine system components of their power plants, the heat and mass balance of the turbine system will change. This system has ability to detect the abnormal signs of their components by finding the changes of the heat and mass balance. Moreover we note that this system is built for steam turbine cycle operating with saturated steam conditions. (author)

  19. Architecture Support for Runtime Integration and Verification of Component-based Systems of Systems

    NARCIS (Netherlands)

    Gonzalez, A.; Piel, E.; Gross, H.G.

    2008-01-01

    Preprint of paper published in: ASE 2008 - 23rd IEEE/ACM International Conference on Automated Software Engineering, 15-19 September 2008; doi:10.1109/ASEW.2008.4686292 Systems-of-Systems (SoS) represent a novel kind of system, for which runtime evolution is a key requirement, as components join and

  20. Complement propriety and conspiracy in nanomedicine

    DEFF Research Database (Denmark)

    Moghimi, Seyed Moein

    2016-01-01

    The complement system is the first line of body's defense against intruders and it acts as a functional bridge between innate and adaptive arms of the immune system. This commentary examines the key roles of complement activation in response to nanomedicine administration, including nucleic acid...... complexes. These comprise beneficial (eg, adjuvanticity) as well as adverse effects (eg, infusion-related reactions). Pigs (and sheep) are often used as predictive models of nanomedicine-mediated infusion-related reactions in humans. The validity of these models in relation to human responses is questioned...

  1. Stop and Restart Effects on Modern Vehicle Starting System Components

    Energy Technology Data Exchange (ETDEWEB)

    Windover, Paul R. [Argonne National Lab. (ANL), Argonne, IL (United States); Owens, Russell J. [Argonne National Lab. (ANL), Argonne, IL (United States); Levinson, Terry M. [Argonne National Lab. (ANL), Argonne, IL (United States); Laughlin, Michael [Argonne National Lab. (ANL), Argonne, IL (United States); Gaines, Linda [Argonne National Lab. (ANL), Argonne, IL (United States)

    2015-01-01

    Many drivers of personal and commercial vehicles believe that turning the vehicle off and on frequently instead of idling will cause premature wear of the starter system (starter motor and starter battery). As a result, they are concerned that the replacement cost of the starter motor and/or battery due to increased manual engine cycling would be more than the cumulative cost of the fuel saved by not idling unnecessarily. A number of variables play a role in addressing this complex concern, including the number of starting cycles per day, the time between starting cycles, the intended design life of the starting system, the amount of fuel used to restart an engine, and the cumulative cost of the saved fuel. Qualitative and quantitative information from a variety of sources was used to develop a life-cycle economic model to evaluate the cost and quantify the realistic factors that are related to the permissible frequency of starter motor cycles for the average vehicle to economically minimize engine idle time. Annual cost savings can be calculated depending on shutdown duration and the number of shutdown cycles per day. Analysis shows that cost savings are realized by eliminating idling exceeding one minute by shutting down the engine and restarting it. For a typical motorist, the damage to starting system components resulting from additional daily start cycles will be negligible. Overall, it was found that starter life is mostly dependent on the total number of start cycles, while battery life is more dependent on ensuring a full charge between start events.

  2. Development of a component centered fault monitoring and diagnosis knowledge based system for space power system

    Science.gov (United States)

    Lee, S. C.; Lollar, Louis F.

    1988-01-01

    The overall approach currently being taken in the development of AMPERES (Autonomously Managed Power System Extendable Real-time Expert System), a knowledge-based expert system for fault monitoring and diagnosis of space power systems, is discussed. The system architecture, knowledge representation, and fault monitoring and diagnosis strategy are examined. A 'component-centered' approach developed in this project is described. Critical issues requiring further study are identified.

  3. Pipeline protection with multi component liquid polyurethane coating systems

    Energy Technology Data Exchange (ETDEWEB)

    Kuprion, Rainer; Hornig, Maja [TIB Chemicals Ag, Mannheim (Germany)

    2009-07-01

    Protective coating systems are one of the major defence mechanisms against corrosion for transmission pipelines, pipes within a refinery or petrochemical processing facilities. More and more pipelines are being constructed for the supply and transmission of gas and oil, each year but, in addition many existing pipelines are approaching an age where inspection reveals the necessity to consider complete refurbishment. However, the number of rehabilitation projects each year is still relatively small. Therefore, in the coming years, a rising need and necessity can be expected, for the owners and operating companies to be faced with the option of either replacing the pipeline or refurbishing of the existing pipeline. If the pipeline is known to have external corrosion, then safe and economic operation should be assured. Rehabilitation should be done before it is too late in order to ensure its future integrity and operational life. Rehabilitation of pipelines has been both the economic solution and, more significantly, the ecological solution and in many of those cases the coatings selected for the external protection has been multi component liquids based on 100% solids polyurethanes. (author)

  4. Intercrystalline internal adsorption in systems with limiting solubility of components

    International Nuclear Information System (INIS)

    Krysova, S.K.; Stepanova, V.A.; Mozgovoj, M.V.

    1979-01-01

    The decrease of the excessive energy of the intercrystalline boundary of ion by additions of transitional elements having unlimited solubility in iron has been studied. The data obtained agree with the results of an earlier work based on materials of a less higher initial purity. For the systems studied (Fe-V, Fe-Cr, Fe-Mn, Fe-Ni) the degree of the intercrystalline interval adsorption is independent of either the annealing temperature or the cooling method. This corresponds to the notion of the relationship between the intercrystalline internal adsorption and the cubic solubility of the addition in a given solvent. For pure ion, a weak temperature dependence of the excessive energy of the intercrystalline boundries was found in the lower section of the examined temperature range. The constants of cumulative recrystallization of the alloys studied in α-phase show a stepwise dependence upon the atomic number of the solute component, what indicates the relationship between the cumulative recrystallization and the intercrystalline internal adsorption. A monotonous decrease of the constant of cumulative recrystallization is observed for the same alloys in α-phase, on both sides of iron

  5. Safety design requirements for safety systems and components of JSFR

    International Nuclear Information System (INIS)

    Kubo, Shigenobu; Shimakawa, Yoshio; Yamano, Hidemasa; Kotake, Shoji

    2011-01-01

    Safety design requirements for JSFR were summarized taking the development targets of the FaCT project and design feature of JSFR into account. The related safety principle and requirements for Monju, CRBRP, PRISM, SPX, LWRs, IAEA standards, goals of GIF, basic principle of INPRO etc. were also taken into account so that the safety design requirements can be a next-generation global standard. The development targets for safety and reliability are set based on those of FaCT, namely, ensuring safety and reliability equal to future LWR and related fuel cycle facilities. In order to achieve these targets, the defence-in-depth concept is used as the basic safety design principle. General features of the safety design requirements are 1) Achievement of higher reliability, 2) Achievement of higher inspectability and maintainability, 3) Introduction of passive safety features, 4) Reduction of operator action needs, 5) Design consideration against Beyond Design Basis Events, 6) In-Vessel Retention of degraded core materials, 7) Prevention and mitigation against sodium chemical reactions, and 8) Design against external events. The current specific requirements for each system and component are summarized taking the basic design concept of JSFR into account, which is an advanced loop-type large-output power plant with a mixed-oxide-fuelled core. (author)

  6. Optimization of Thermoelectric Components for Automobile Waste Heat Recovery Systems

    Science.gov (United States)

    Kumar, Sumeet; Heister, Stephen D.; Xu, Xianfan; Salvador, James R.

    2015-10-01

    For a typical spark ignition engine approximately 40% of available thermal energy is lost as hot exhaust gas. To improve fuel economy, researchers are currently evaluating technology which exploits exhaust stream thermal power by use of thermoelectric generators (TEGs) that operate on the basis of the Seebeck effect. A 5% improvement in fuel economy, achieved by use of TEG output power, is a stated objective for light-duty trucks and personal automobiles. System modeling of thermoelectric (TE) components requires solution of coupled thermal and electric fluxes through the n and p-type semiconductor legs, given appropriate thermal boundary conditions at the junctions. Such applications have large thermal gradients along the semiconductor legs, and material properties are highly dependent on spatially varying temperature profiles. In this work, one-dimensional heat flux and temperature variations across thermoelectric legs were solved by using an iterative numerical approach to optimize both TE module and TEG designs. Design traits were investigated by assuming use of skutterudite as a thermoelectric material with potential for automotive applications in which exhaust gas and heat exchanger temperatures typically vary from 100°C to over 600°C. Dependence of leg efficiency, thermal fluxes and electric power generation on leg geometry, fill fractions, electric current, thermal boundary conditions, etc., were studied in detail. Optimum leg geometries were computed for a variety of automotive exhaust conditions.

  7. Complement, a target for therapy in inflammatory and degenerative diseases.

    Science.gov (United States)

    Morgan, B Paul; Harris, Claire L

    2015-12-01

    The complement system is a key innate immune defence against infection and an important driver of inflammation; however, these very properties can also cause harm. Inappropriate or uncontrolled activation of complement can cause local and/or systemic inflammation, tissue damage and disease. Complement provides numerous options for drug development as it is a proteolytic cascade that involves nine specific proteases, unique multimolecular activation and lytic complexes, an arsenal of natural inhibitors, and numerous receptors that bind to activation fragments. Drug design is facilitated by the increasingly detailed structural understanding of the molecules involved in the complement system. Only two anti-complement drugs are currently on the market, but many more are being developed for diseases that include infectious, inflammatory, degenerative, traumatic and neoplastic disorders. In this Review, we describe the history, current landscape and future directions for anti-complement therapies.

  8. Complement and hyper acute rejection

    Directory of Open Access Journals (Sweden)

    Al-Rabia Mohammed

    2009-01-01

    Full Text Available Organ transplantation has been a major development in clinical medicine but its success has been marred by the immune system′s capacity to respond to "non-self" cells and tissues. A full molecular understanding of this mechanism and the myriad triggers for immune rejection is yet to be elucidated. Consequently, immunosuppressive drugs remain the mainstay of post-transplant ma-nagement; however, these interventions have side effects such as increased incidence of cancer, post-transplant lymphoproliferative disorders, susceptibility to infection if not managed appro-priately and the inconvenience to the patient of lifelong treatment. Novel therapeutic approaches based on molecular understanding of immunological processes are thus needed in this field. The notion that factors influencing successful transplants might be of use as therapeutic approaches is both scientifically and medically appealing. Recent developments in the understanding of successful transplants are expected to provide new opportunities for safer transplantation. This article reviews the present understanding of the molecular basis of rejection and the role of complement in this process as well as the possibility of generating "intelligent" therapy that better target crucial components of hyper-acute rejections.

  9. BF*F allotype of the alternative pathway of complement: A marker of protection against the development of antiphospholipid antibodies in patients with systemic lupus erythematosus.

    Science.gov (United States)

    Picceli, V F; Skare, T L; Nisihara, R M; Nass, F R; Messias-Reason, I T; Utiyama, S R R

    2016-04-01

    B factor (BF) from the alternative complement pathway seems to participate in the pathophysiology of systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). To study the allotypic variability of BF in SLE and their associations with clinical and autoantibodies profile. BF allotypes were determined by high-voltage agarose gel electrophoresis, under constant cooling, followed by immunofixation with anti-human BF antibody, in 188 SLE patients and 103 controls. Clinical and serological data were obtained from medical examination and records. No significant differences of BF variants between patients and controls were found, neither in relation to epidemiologic or clinical manifestations. Associations of phenotype BF SS07 and allotype BF*S07 were found with anticardiolipin IgM (aCl-IgM) antibodies (p = 0.014 and p = 0.009 respectively), but not with aCl-IgG, lupus anticoagulant (LA), anti β2GPI or clinical APS. A significant decrease in BF*F allotype (p = 0.043) and BF SF phenotype (p = 0.018) was detected in patients with anti-phospholipid antibodies as a whole (aCl-IgG, aCl-IgM, LA and anti β2GPI). There is a link between phenotype BF SS07 and allotype BF*S07 with aCl-IgM in SLE patients; BF*F allotype could be considered a marker of protection against the development of antiphospholipid antibodies in these patients. © The Author(s) 2015.

  10. Decision Support System and Customer Relationship Management as Components of the Cybernetic System Enterprise

    Directory of Open Access Journals (Sweden)

    Ruxandra MISDOLEA

    2010-01-01

    Full Text Available This study analyzes the role played by the information system and its component, the software system, in a larger system - the Enterprise. In this context, the paper focuses on the structure of Decision Support System and Customer Relationship Management and their benefits in the functioning of the global system, by examining the conditions of implementation of these tools in the organization. We will show that used independently these tools offer reduced services, but when interconnected, they become a very powerful tool for command and control. Viability, evolution and autonomy requested by users for their information system are obtained more easily by a systemic-cybernetic approach to the Enterprise.

  11. Concept of control and modifying systems of accelerator components; Kontseptsiya sistem avtomatizatsii kontrolya i upravleniya

    Energy Technology Data Exchange (ETDEWEB)

    Gudkov, K A; Chepurnov, A S; Shumakov, A V [NNIYaF MGU, Moskva (Russian Federation)

    1996-12-31

    Paper considers the main trends of development of automated control and monitoring systems of accelerator components. Application of communication ring structures will enable to design highly efficient object-oriented systems of automation of research and process components. 5 refs.

  12. Complement-coagulation cross-talk: a potential mediator of the physiological activation of complement by low pH

    Directory of Open Access Journals (Sweden)

    Hany Ibrahim Kenawy

    2015-05-01

    Full Text Available The complement system is a major constituent of the innate immune system. It not only bridges innate and adaptive arms of the immune system but also links the immune system with the coagulation system. Current understanding of the role of complement has extended far beyond fighting of infections, and now encompasses maintenance of homeostasis, tissue regeneration and pathophysiology of multiple diseases. It has been known for many years that complement activation is strongly pH sensitive, but only relatively recently has the physiological significance of this been appreciated. Most complement assays are carried out at the physiological pH 7.4. However, pH in some extracellular compartments, for example renal tubular fluid in parts of the tubule, and extracellular fluid at inflammation loci, is sufficiently acidic to activate complement. The exact molecular mechanism of this activation is still unclear, but possible cross talk between the contact system and complement may exist at low pH with subsequent complement activation. The current article reviews the published data on the effect of pH on the contact system and complement activity, the nature of the pH sensor molecules, and the clinical implications of these effects. Of particular interest is chronic kidney disease (CKD accompanied by metabolic acidosis, in which therapeutic alkalinisation of urine has been shown significantly to reduce tubular complement activation products, an effect which may have important implications for slowing progression of CKD.

  13. Valores séricos de imunoglobulinas e dos componentes do complemento em gestantes com ruptura prematura de membranas Immunoglobulin serum values and complement components in pregnant women with premature rupture of the membranes

    Directory of Open Access Journals (Sweden)

    Valquíria Roveran

    2007-04-01

    imunoglobulinas e complementos.PURPOSE: the premature rupture of membranes (PROM has been a reason for many investigations, amongst which the involved immune mechanisms. Ahead of the scarcity of studies related to the subject, this work had as objective to evaluate the serum values of IgA, IgG, IgM, C3 and C4 in pregnant women with pre-term PROM. METHODS: in this transversal study, 36 pregnant women had been enclosed, with gestational age between 23 and 37 weeks. Of this total, 15 women had had laboratorial and clinical diagnosis of PROM. Patients with beginning of the childbirth work, clinical signals of infection, clinical dysfunction with systemic repercussion had been excluded. Serum concentrations of immunoglobulin (IgA, immunoglobulin M (IgM and immunoglobulin G (IgG, C3 and C4 had been evaluated in the patients with (study group and without PROM (control group. Correlation among dosages; number of childbirths and time of rupture was determined by Spearman coefficient correlation (r value. RESULTS: serum levels of IgA (average±SD had been significantly higher in the patients of the control group (271.0±107.0 versus 202.9±66.1; respectively, control and study group; p=0.024. There was no statistical difference when the levels of IgM, IgG, C3 and C4 had been compared between two groups. Significant association was not noticed between the number of childbirths and the IgA, IgM, IgG, C3 and C4 dosages (Spearman; r between -0,009 and 0,027; p>0,05. The average time of rupture of study group patients was of 19.1 hours (one - 72 hours, without association with the evaluated serum dosages. CONCLUSIONS: pregnant women with PROM show levels of IgA significantly lower than normal pregnant patients. The variable "number of childbirths" does not act as a factor of confusion in the comparative analysis of the dosages obtained in patients with or without PROM, as well as also it did not have association between the time of rupture and the immunoglobulin and complements serum dosages.

  14. LIRA - License Renewal Assistant an expert system advisor for system and component screening

    International Nuclear Information System (INIS)

    Wood, R.M.; DeLuke, R.J.; Lu, Yi; Catron, S.R.

    1992-01-01

    In developing a license renewal application for a nuclear power plant, it is necessary to identify those systems and components for which age-related degradation must be evaluated and addressed in detail. One approach, used in the Monticello Lead Plant project, is to screen all plant systems and components, based on criteria developed by the Nuclear Utility Management and Resources Council (NUMARC). This paper describes an expert system developed as an assistant in the application of the screening methodology. 4 refs., 5 figs., 1 tab

  15. The fractional virial potential energy in two-component systems

    Directory of Open Access Journals (Sweden)

    Caimmi R.

    2008-01-01

    Full Text Available Two-component systems are conceived as macrogases, and the related equation of state is expressed using the virial theorem for subsystems, under the restriction of homeoidally striated density profiles. Explicit calculations are performed for a useful reference case and a few cases of astrophysical interest, both with and without truncation radius. Shallower density profiles are found to yield an equation of state, φ = φ(y, m, characterized (for assigned values of the fractional mass, m = Mj /Mi by the occurrence of two extremum points, a minimum and a maximum, as found in an earlier attempt. Steeper density profiles produce a similar equation of state, which implies that a special value of m is related to a critical curve where the above mentioned extremum points reduce to a single horizontal inflexion point, and curves below the critical one show no extremum points. The similarity of the isofractional mass curves to van der Waals' isothermal curves, suggests the possibility of a phase transition in a bell-shaped region of the (Oyφ plane, where the fractional truncation radius along a selected direction is y = Rj /Ri , and the fractional virial potential energy is φ = (Eji vir /(Eij vir . Further investigation is devoted to mass distributions described by Hernquist (1990 density profiles, for which an additional relation can be used to represent a sample of N = 16 elliptical galaxies (EGs on the (Oyφ plane. Even if the evolution of elliptical galaxies and their hosting dark matter (DM haloes, in the light of the model, has been characterized by equal fractional mass, m, and equal scaled truncation radius, or concentration, Ξu = Ru /r† , u = i, j, still it cannot be considered as strictly homologous, due to different values of fractional truncation radii, y, or fractional scaling radii, y† = r† /r† , deduced from sample objects.

  16. Classification of structural component and degradation mechanisms for containment systems

    International Nuclear Information System (INIS)

    Judge, R.C.B.

    1994-01-01

    UK licence requirements for operation of nuclear power plants is dependent, inter alia, upon the licensee making and implementing adequate arrangements for the regular and systematic examination, inspection, maintenance and testing of all plant which may affect safety (Licence Condition 28). Similarly, the US NRC's Maintenance Rule (published in 10CFR50.65) specifies that a maintenance programme should be developed for plant systems, structures and components determined to be sensitive to ageing which will be used for the balance of the current (and, if relevant, extended) operating licence period. Against this background, the plant operators are seeking to minimise operating and maintenance costs and to enhance plant availability. This leads to a need to optimise the plant inspection and monitoring regimes whilst meeting regulatory requirements. In this paper, a conceptual framework for classifying civil structures and significant ageing mechanisms is described. This provides a systematic approach to making quantitative assessments of the likelihood and of potential degradation mechanisms and forms a consistent framework and a logical basis for prioritising inspection and maintenance schedules. The proposed method is analogous to a fault tree assessment, in which the likelihood of degradation due to a specific mechanism is considered as an event. The structures are considered in terms of their subcomponents. For each subcomponent, the value assigned to the likelihood of degradation is progressively reduced by a sequence of factors which make allowance for the structural and safety significance of any degradation and for the potential for timely detection of any degradation. Illustrative values for these factors are quoted in the text; it is recommended that these values are reviewed following a trial application of the method. (author)

  17. Activation of Human Complement System by Dextran-Coated Iron Oxide Nanoparticles Is Not Affected by Dextran/Fe Ratio, Hydroxyl Modifications, and Crosslinking

    DEFF Research Database (Denmark)

    Wang, Guankui; Chen, Fangfang; Banda, Nirmal K

    2016-01-01

    While having tremendous potential as therapeutic and imaging tools, the clinical use of engineered nanoparticles has been associated with serious safety concerns. Activation of the complement cascade and the release of proinflammatory factors C3a and C5a may contribute to infusion-related reactio...

  18. Brazilian two-component TLD albedo neutron individual monitoring system

    Energy Technology Data Exchange (ETDEWEB)

    Martins, M.M., E-mail: marcelo@ird.gov.b [Instituto de Radioprotecao e Dosimetria (IRD), Av. Salvador Allende, s/n, CEP: 22780-160, Rio de Janeiro, RJ (Brazil); Mauricio, C.L.P., E-mail: claudia@ird.gov.b [Instituto de Radioprotecao e Dosimetria (IRD), Av. Salvador Allende, s/n, CEP: 22780-160, Rio de Janeiro, RJ (Brazil); Fonseca, E.S. da, E-mail: evaldo@ird.gov.b [Instituto de Radioprotecao e Dosimetria (IRD), Av. Salvador Allende, s/n, CEP: 22780-160, Rio de Janeiro, RJ (Brazil); Silva, A.X. da, E-mail: ademir@con.ufrj.b [Coordenacao dos Programas de Pos-Graduacao em Engenharia, COPPE/PEN Caixa Postal 68509, CEP: 21941-972, Rio de Janeiro, RJ (Brazil)

    2010-12-15

    Since 1983, Instituto de Radioprotecao e Dosimetria, Brazil, uses a TLD one-component albedo neutron monitor, which has a single different calibration factor specifically for each installation type. In order to improve its energy response, a two-component albedo monitor was developed, which measure the thermal neutron component besides the albedo one. The two-component monitor has been calibrated in reference neutron fields: thermal, five accelerator-produced monoenergetic beams (70, 144, 565, 1200 and 5000 keV) and five radionuclide sources ({sup 252}Cf, {sup 252}Cf(D{sub 2}O), {sup 241}Am-Be, {sup 241}Am-B and {sup 238}Pu-Be) at several distances. Since January 2008, mainly Brazilian workers who handle neutron sources at different distances and moderation, such as in well logging and calibration facilities are using it routinely.

  19. Design of sodium cooled reactor systems and components for maintainability

    International Nuclear Information System (INIS)

    Carr, R.W.; Charnock, H.O.; McBride, J.P.

    1978-09-01

    Special maintenability problems associated with the design and operation of sodium cooled reactor plants are discussed. Some examples of both good and bad design practice are introduced from the design of the FFTF plant and other plants. Subjects include design for drainage, cleaning, decontamination, access, component removal, component disassembly and reassembly, remote tooling, jigs, fixtures, and design for minimizing radiation exposure of maintenance personnel. Check lists are included

  20. Kupffer cell complement receptor clearance function and host defense.

    Science.gov (United States)

    Loegering, D J

    1986-01-01

    Kupffer cells are well known to be important for normal host defense function. The development of methods to evaluate the in vivo function of specific receptors on Kupffer cells has made it possible to assess the role of these receptors in host defense. The rationale for studying complement receptors is based on the proposed important role of these receptors in host defense and on the observation that the hereditary deficiency of a complement receptor is associated with recurrent severe bacterial infections. The studies reviewed here demonstrate that forms of injury that are associated with depressed host defense including thermal injury, hemorrhagic shock, trauma, and surgery also cause a decrease in complement receptor clearance function. This decrease in Kupffer cell receptor clearance function was shown not to be the result of depressed hepatic blood flow or depletion of complement components. Complement receptor function was also depressed following the phagocytosis of particulates that are known to depress Kupffer cell host defense function. Endotoxemia and bacteremia also were associated with a depression of complement receptor function. Complement receptor function was experimentally depressed in uninjured animals by the phagocytosis of IgG-coated erythrocytes. There was a close association between the depression of complement receptor clearance function and increased susceptibility to the lethal effects of endotoxin and bacterial infection. These studies support the hypotheses that complement receptors on Kupffer cells are important for normal host defense and that depression of the function of these receptors impairs host defense.

  1. Development of the interactive model between Component Cooling Water System and Containment Cooling System using GOTHIC

    International Nuclear Information System (INIS)

    Byun, Choong Sup; Song, Dong Soo; Jun, Hwang Yong

    2006-01-01

    In a design point of view, component cooling water (CCW) system is not full-interactively designed with its heat loads. Heat loads are calculated from the CCW design flow and temperature condition which is determined with conservatism. Then the CCW heat exchanger is sized by using total maximized heat loads from above calculation. This approach does not give the optimized performance results and the exact trends of CCW system and the loads during transient. Therefore a combined model for performance analysis of containment and the component cooling water(CCW) system is developed by using GOTHIC software code. The model is verified by using the design parameters of component cooling water heat exchanger and the heat loads during the recirculation mode of loss of coolant accident scenario. This model may be used for calculating the realistic containment response and CCW performance, and increasing the ultimate heat sink temperature limits

  2. The Fractional Virial Potential Energy in Two-Component Systems

    Directory of Open Access Journals (Sweden)

    Caimmi, R.

    2008-12-01

    Full Text Available Two-component systems are conceived as macrogases, and the related equation of state is expressed using the virial theorem for subsystems, under the restriction of homeoidally striated density profiles. Explicit calculations are performed for a useful reference case and a few cases of astrophysical interest, both with and without truncation radius. Shallower density profiles are found to yield an equation of state, $phi=phi(y,m$, characterized (for assigned values of the fractional mass, $m=M_j/ M_i$ by the occurrence of two extremum points, a minimum and a maximum, as found in an earlier attempt. Steeper density profiles produce a similar equation of state, which implies that a special value of $m$ is related to a critical curve where the above mentioned extremum points reduce to a single horizontal inflexion point, and curves below the critical one show no extremum points. The similarity of the isofractional mass curves to van der Waals' isothermal curves, suggests the possibility of a phase transition in a bell-shaped region of the $({sf O}yphi$ plane, where the fractional truncation radius along a selected direction is $y=R_j/R_i$, and the fractional virial potential energy is $phi=(E_{ji}_mathrm{vir}/(E_{ij}_mathrm{vir}$. Further investigation is devoted to mass distributions described by Hernquist (1990 density profiles, for which an additional relation can be used to represent a sample of $N=16$ elliptical galaxies (EGs on the $({sf O}yphi$ plane. Even if the evolution of elliptical galaxies and their hosting dark matter (DM haloes, in the light of the model, has been characterized by equal fractional mass, $m$, and equal scaled truncation radius, or concentration, $Xi_u=R_u/r_u^dagger$, $u=i,j$, still it cannot be considered as strictly homologous, due to different values of fractional truncation radii, $y$, or fractional scaling radii, $y^dagger=r_j^dagger/r_i^dagger$, deduced from sample objects.

  3. Complement factor H deficiency results in decreased neuroretinal expression of Cd59a in aged mice

    DEFF Research Database (Denmark)

    Faber, Carsten; Williams, Jennifer; Juel, Helene Bæk

    2012-01-01

    Purpose. The complement system is closely linked to the pathogenesis of AMD. Several complement genes are expressed in RPE, and complement proteins accumulate in drusen. Further, a common variant of complement factor H (CFH) confers increased risk of developing AMD. Because the mechanisms by which...

  4. Development of the software for the component reliability database system of Korean nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Han, Sang Hoon; Kim, Seung Hwan; Choi, Sun Young [Korea Atomic Energy Research Institute, Taejeon (Korea)

    2002-03-01

    A study was performed to develop the system for the component reliability database which consists of database system to store the reliability data and softwares to analyze the reliability data.This system is a part of KIND (Korea Information System for Nuclear Reliability Database).The MS-SQL database is used to stores the component population data, component maintenance history, and the results of reliability analysis. Two softwares were developed for the component reliability system. One is the KIND-InfoView for the data storing, retrieving and searching. The other is the KIND-CompRel for the statistical analysis of component reliability. 4 refs., 13 figs., 7 tabs. (Author)

  5. Mechanisms of complement activation by dextran-coated superparamagnetic iron oxide (SPIO) nanoworms in mouse versus human serum

    DEFF Research Database (Denmark)

    Banda, Nirmal K; Mehta, Gaurav; Chao, Ying

    2014-01-01

    BACKGROUND: The complement system is a key component of innate immunity implicated in the neutralization and clearance of invading pathogens. Dextran coated superparamagnetic iron oxide (SPIO) nanoparticle is a promising magnetic resonance imaging (MRI) contrast agent. However, dextran SPIO has...... the mechanisms of human complement activation. Mouse data were analyzed by non-paired t-test, human data were analyzed by ANOVA followed by multiple comparisons with Student-Newman-Keuls test. RESULTS: In mouse sera, SPIO NW triggered the complement activation via the LP, whereas the AP contributes via...... the CP, but that did not affect the total level of C3 deposition on the particles. CONCLUSIONS: There were important differences and similarities in the complement activation by SPIO NW in mouse versus human sera. Understanding the mechanisms of immune recognition of nanoparticles in mouse and human...

  6. Model-integrating software components engineering flexible software systems

    CERN Document Server

    Derakhshanmanesh, Mahdi

    2015-01-01

    In his study, Mahdi Derakhshanmanesh builds on the state of the art in modeling by proposing to integrate models into running software on the component-level without translating them to code. Such so-called model-integrating software exploits all advantages of models: models implicitly support a good separation of concerns, they are self-documenting and thus improve understandability and maintainability and in contrast to model-driven approaches there is no synchronization problem anymore between the models and the code generated from them. Using model-integrating components, software will be

  7. Effect of Component Failures on Economics of Distributed Photovoltaic Systems

    Energy Technology Data Exchange (ETDEWEB)

    Lubin, Barry T. [Univ. of Hartford, West Hartford, CT (United States)

    2012-02-02

    This report describes an applied research program to assess the realistic costs of grid connected photovoltaic (PV) installations. A Board of Advisors was assembled that included management from the regional electric power utilities, as well as other participants from companies that work in the electric power industry. Although the program started with the intention of addressing effective load carrying capacity (ELCC) for utility-owned photovoltaic installations, results from the literature study and recommendations from the Board of Advisors led investigators to the conclusion that obtaining effective data for this analysis would be difficult, if not impossible. The effort was then re-focused on assessing the realistic costs and economic valuations of grid-connected PV installations. The 17 kW PV installation on the University of Hartford's Lincoln Theater was used as one source of actual data. The change in objective required a more technically oriented group. The re-organized working group (changes made due to the need for more technically oriented participants) made site visits to medium-sized PV installations in Connecticut with the objective of developing sources of operating histories. An extensive literature review helped to focus efforts in several technical and economic subjects. The objective of determining the consequences of component failures on both generation and economic returns required three analyses. The first was a Monte-Carlo-based simulation model for failure occurrences and the resulting downtime. Published failure data, though limited, was used to verify the results. A second model was developed to predict the reduction in or loss of electrical generation related to the downtime due to these failures. Finally, a comprehensive economic analysis, including these failures, was developed to determine realistic net present values of installed PV arrays. Two types of societal benefits were explored, with quantitative valuations developed

  8. Handbook of driver assistance systems basic information, components and systems for active safety and comfort

    CERN Document Server

    Hakuli, Stephan; Lotz, Felix; Singer, Christina

    2016-01-01

    This fundamental work explains in detail systems for active safety and driver assistance, considering both their structure and their function. These include the well-known standard systems such as Anti-lock braking system (ABS), Electronic Stability Control (ESC) or Adaptive Cruise Control (ACC). But it includes also new systems for protecting collisions protection, for changing the lane, or for convenient parking. The book aims at giving a complete picture focusing on the entire system. First, it describes the components which are necessary for assistance systems, such as sensors, actuators, mechatronic subsystems, and control elements. Then, it explains key features for the user-friendly design of human-machine interfaces between driver and assistance system. Finally, important characteristic features of driver assistance systems for particular vehicles are presented: Systems for commercial vehicles and motorcycles.

  9. A Resource-Aware Component Model for Embedded Systems

    OpenAIRE

    Vulgarakis, Aneta

    2009-01-01

    Embedded systems are microprocessor-based systems that cover a large range of computer systems from ultra small computer-based devices to large systems monitoring and controlling complex processes. The particular constraints that must be met by embedded systems, such as timeliness, resource-use efficiency, short time-to-market and low cost, coupled with the increasing complexity of embedded system software, demand technologies and processes that will tackle these issues. An attractive approac...

  10. Complementing the sugar code: role of GAGs and sialic acid in complement regulation

    Directory of Open Access Journals (Sweden)

    Alex eLangford-Smith

    2015-02-01

    Full Text Available Sugar molecules play a vital role on both microbial and mammalian cells, where they are involved in cellular communication, govern microbial virulence and modulate host immunity and inflammatory responses. The complement cascade, as part of a host’s innate immune system, is a potent weapon against invading bacteria but has to be tightly regulated to prevent inappropriate attack and damage to host tissues. A number of complement regulators, such as factor H and properdin, interact with sugar molecules, such as glycosaminoglycans and sialic acid, on host and pathogen membranes and direct the appropriate complement response by either promoting the binding of complement activators or inhibitors. The binding of these complement regulators to sugar molecules can vary from location to location, due to their different specificities and because distinct structural and functional subpopulations of sugars are found in different human organs, such as the brain, kidney and eye. This review will cover recent studies that have provided important new insights into the role of glycosaminoglycans and sialic acid in complement regulation and how sugar recognition may be compromised in disease

  11. Complement activation by ceramide transporter proteins.

    Science.gov (United States)

    Bode, Gerard H; Losen, Mario; Buurman, Wim A; Veerhuis, Robert; Molenaar, Peter C; Steinbusch, Harry W M; De Baets, Marc H; Daha, Mohamed R; Martinez-Martinez, Pilar

    2014-02-01

    C1q is the initiator of the classical complement pathway and, as such, is essential for efficient opsonization and clearance of pathogens, altered self-structures, and apoptotic cells. The ceramide transporter protein (CERT) and its longer splicing isoform CERTL are known to interact with extracellular matrix components, such as type IV collagen, and with the innate immune protein serum amyloid P. In this article, we report a novel function of CERT in the innate immune response. Both CERT isoforms, when immobilized, were found to bind the globular head region of C1q and to initiate the classical complement pathway, leading to activation of C4 and C3, as well as generation of the membrane attack complex C5b-9. In addition, C1q was shown to bind to endogenous CERTL on the surface of apoptotic cells. These results demonstrate the role of CERTs in innate immunity, especially in the clearance of apoptotic cells.

  12. Diffusion of elements and vacancies in multi-component systems

    Czech Academy of Sciences Publication Activity Database

    Fischer, F. D.; Svoboda, Jiří

    2014-01-01

    Roč. 60, MAR (2014), s. 338-367 ISSN 0079-6425 Institutional support: RVO:68081723 Keywords : multi-component diffusion * vacancy activity * manning theory * stress-driven diffusion Subject RIV: BJ - Thermodynamics Impact factor: 27.417, year: 2014

  13. Steam generators: critical components in nuclear steam supply systems

    Energy Technology Data Exchange (ETDEWEB)

    Stevens-Guille, P D

    1974-02-28

    Steam generators are critical components in power reactors. Even small internal leaks result in costly shutdowns for repair. Surveys show that leaks have affected one half of all water-cooled reactors in the world with steam generators. CANDU reactors have demonstrated the highest reliability. However, AECL is actively evolving new technology in design, manufacture, inspection and operation to maintain reliability. (auth)

  14. Safety assessment of primary system components at the USNRC

    Energy Technology Data Exchange (ETDEWEB)

    Serpan, C Z; Chen, C Y; Taboada, A

    1988-12-31

    This document deals with the safety assessment in nuclear reactor components at the USNRC. The USNRC regulations and requirements concerning nuclear reactor design and operations are presented, together with guides and standards which describe how the actions should be implemented. The safety assessment relies on fracture analysis and Non Destructive Examination (NDE). (TEC).

  15. Structured automated code checking through structural components and systems engineering

    NARCIS (Netherlands)

    Coenders, J.L.; Rolvink, A.

    2014-01-01

    This paper presents a proposal to employ the design computing methodology proposed as StructuralComponents (Rolvink et al [6] and van de Weerd et al [7]) as a method to perform a digital verification process to fulfil the requirements related to structural design and engineering as part of a

  16. Magnetic field compatibility of components for ITER electrical distribution systems

    International Nuclear Information System (INIS)

    Hourtoule, J.; Van Houtte, D.; Hertout, P.; Fejoz, P.

    2004-01-01

    The electrical distribution boards and control cubicles, installed inside the ITER Tokamak building, can be subject to a constant or slowly variable magnetic field up to 70 mT, 10 mT/s induced by ITER coils. This is a very unusual environmental condition and there are almost no data available on static magnetic field compatibility for the standard industrial electrical and electronic components that might be used inside standard low voltage distribution boards and standard control boards. CEA has especially taken in charge the test of electronic, control and signal conditioning units. For this purpose a test bed composed of a solenoid and a 30 V, 800 A power supply, has been developed at CEA Cadarache. Its characteristics are the following: - Magnetic field capability: 40 mT; - Variation of field: up to 10 mT/s. Useful dimensions for equipment under test: 500 x 500 x 500 mm. The list of the components to be tested has been identified trying to find common and recent components of different manufacturers. Test procedures have been written following the most relevant IEC standards and manufacturing recommendations and procedures. Magnetic compatibility tests on the basic components of standard low voltage control boards has been performed. All components tested are more or less sensitive to magnetic fields and the effect varies from the simple perturbation of the output signals to the partial destruction of some electronic card. The most sensitive are the ferromagnetic components like small power supplies transformers, relays, on inductors. For power supplies the most important consequence is the increase of the consumption (primary current). For most of the conditioning units the operational limits were found in the range of 30 mT (destruction of materials and overload of the supply) For PLC (Programme Logic Control) central units tested, the limit is in the order of 40 mT (components permanently out of service) Relays are limited to 15 mT (outputs oscillations, no

  17. Experiences integrating autonomous components and legacy systems into tsunami early warning systems

    Science.gov (United States)

    Reißland, S.; Herrnkind, S.; Guenther, M.; Babeyko, A.; Comoglu, M.; Hammitzsch, M.

    2012-04-01

    Fostered by and embedded in the general development of Information and Communication Technology (ICT) the evolution of Tsunami Early Warning Systems (TEWS) shows a significant development from seismic-centred to multi-sensor system architectures using additional sensors, e.g. sea level stations for the detection of tsunami waves and GPS stations for the detection of ground displacements. Furthermore, the design and implementation of a robust and scalable service infrastructure supporting the integration and utilisation of existing resources serving near real-time data not only includes sensors but also other components and systems offering services such as the delivery of feasible simulations used for forecasting in an imminent tsunami threat. In the context of the development of the German Indonesian Tsunami Early Warning System (GITEWS) and the project Distant Early Warning System (DEWS) a service platform for both sensor integration and warning dissemination has been newly developed and demonstrated. In particular, standards of the Open Geospatial Consortium (OGC) and the Organization for the Advancement of Structured Information Standards (OASIS) have been successfully incorporated. In the project Collaborative, Complex, and Critical Decision-Support in Evolving Crises (TRIDEC) new developments are used to extend the existing platform to realise a component-based technology framework for building distributed TEWS. This talk will describe experiences made in GITEWS, DEWS and TRIDEC while integrating legacy stand-alone systems and newly developed special-purpose software components into TEWS using different software adapters and communication strategies to make the systems work together in a corporate infrastructure. The talk will also cover task management and data conversion between the different systems. Practical approaches and software solutions for the integration of sensors, e.g. providing seismic and sea level data, and utilisation of special

  18. Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells

    DEFF Research Database (Denmark)

    Juel, Helene Bæk; Kaestel, Charlotte; Folkersen, Lasse

    2011-01-01

    In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19...

  19. Exploring agricultural production systems and their fundamental components with system dynamics modeling

    Science.gov (United States)

    Agricultural production in the United States is undergoing marked changes due to rapid shifts in consumer demands, input costs, and concerns for food safety and environmental impact. Agricultural production systems are comprised of multidimensional components and drivers that interact in complex wa...

  20. Maintenance of HVAC-systems and components: How to prevent pollution from HVAC-systems?

    NARCIS (Netherlands)

    Müller, B.; Björkroth, M.; Plitt, U.; Bluyssen, P.M.

    2000-01-01

    In the beginning of 1998 a three-year European project, AIRLESS, was started to develop strategies, principles and protocols to improve and control the performance of HVAC-systems and its components for incorporation in codes and guidelines. Twelve institutes, universities and companies, from seven

  1. Nuclear plant reliability data system. 1979 annual reports of cumulative system and component reliability

    International Nuclear Information System (INIS)

    1979-01-01

    The primary purposes of the information in these reports are the following: to provide operating statistics of safety-related systems within a unit which may be used to compare and evaluate reliability performance and to provide failure mode and failure rate statistics on components which may be used in failure mode effects analysis, fault hazard analysis, probabilistic reliability analysis, and so forth

  2. Complementation of coalgebra automata

    NARCIS (Netherlands)

    Kissig, C.; Venema, Y.

    2009-01-01

    Coalgebra automata, introduced by the second author, generalize the well-known automata that operate on infinite words/streams, trees, graphs or transition systems. This coalgebraic perspective on automata lays foundation to a universal theory of automata operating on infinite models of computation.

  3. Segmented Aperture Interferometric Nulling Testbed (SAINT) II: component systems update

    Science.gov (United States)

    Hicks, Brian A.; Bolcar, Matthew R.; Helmbrecht, Michael A.; Petrone, Peter; Burke, Elliot; Corsetti, James; Dillon, Thomas; Lea, Andrew; Pellicori, Samuel; Sheets, Teresa; Shiri, Ron; Agolli, Jack; DeVries, John; Eberhardt, Andrew; McCabe, Tyler

    2017-09-01

    This work presents updates to the coronagraph and telescope components of the Segmented Aperture Interferometric Nulling Testbed (SAINT). The project pairs an actively-controlled macro-scale segmented mirror with the Visible Nulling Coronagraph (VNC) towards demonstrating capabilities for the future space observatories needed to directly detect and characterize a significant sample of Earth-sized worlds around nearby stars in the quest for identifying those which may be habitable and possibly harbor life. Efforts to improve the VNC wavefront control optics and mechanisms towards repeating narrowband results are described. A narrative is provided for the design of new optical components aimed at enabling broadband performance. Initial work with the hardware and software interface for controlling the segmented telescope mirror is also presented.

  4. Lunar dust transport and potential interactions with power system components

    International Nuclear Information System (INIS)

    Katzan, C.M.; Edwards, J.L.

    1991-11-01

    The lunar surface is covered by a thick blanket of fine dust. This dust may be readily suspended from the surface and transported by a variety of mechanisms. As a consequence, lunar dust can accumulate on sensitive power components, such as photovoltaic arrays and radiator surfaces, reducing their performance. In addition to natural mechanisms, human activities on the Moon will disturb significant amounts of lunar dust. Of all the mechanisms identified, the most serious is rocket launch and landing. The return of components from the Surveyor III provided a rare opportunity to observe the effects of the nearby landing of the Apollo 12 lunar module. The evidence proved that significant dust accumulation occurred on the Surveyor at a distance of 155 m. From available information on particle suspension and transport mechanisms, a series of models was developed to predict dust accumulation as a function of distance from the lunar module. The accumulation distribution was extrapolated to a future lunar lander scenario. These models indicate that accumulation is expected to be substantial even as far as 2 km from the landing site. Estimates of the performance penalties associated with lunar dust coverage on radiators and photovoltaic arrays are presented. Because of the lunar dust adhesive and cohesive properties, the most practical dust defensive strategy appears to be the protection of sensitive components from the arrival of lunar dust by location, orientation, or barriers

  5. Lunar dust transport and potential interactions with power system components

    Energy Technology Data Exchange (ETDEWEB)

    Katzan, C.M.; Edwards, J.L.

    1991-11-01

    The lunar surface is covered by a thick blanket of fine dust. This dust may be readily suspended from the surface and transported by a variety of mechanisms. As a consequence, lunar dust can accumulate on sensitive power components, such as photovoltaic arrays and radiator surfaces, reducing their performance. In addition to natural mechanisms, human activities on the Moon will disturb significant amounts of lunar dust. Of all the mechanisms identified, the most serious is rocket launch and landing. The return of components from the Surveyor III provided a rare opportunity to observe the effects of the nearby landing of the Apollo 12 lunar module. The evidence proved that significant dust accumulation occurred on the Surveyor at a distance of 155 m. From available information on particle suspension and transport mechanisms, a series of models was developed to predict dust accumulation as a function of distance from the lunar module. The accumulation distribution was extrapolated to a future lunar lander scenario. These models indicate that accumulation is expected to be substantial even as far as 2 km from the landing site. Estimates of the performance penalties associated with lunar dust coverage on radiators and photovoltaic arrays are presented. Because of the lunar dust adhesive and cohesive properties, the most practical dust defensive strategy appears to be the protection of sensitive components from the arrival of lunar dust by location, orientation, or barriers.

  6. Probabilistic techniques using Monte Carlo sampling for multi- component system diagnostics

    International Nuclear Information System (INIS)

    Aumeier, S.E.; Lee, J.C.; Akcasu, A.Z.

    1995-01-01

    We outline the structure of a new approach at multi-component system fault diagnostics which utilizes detailed system simulation models, uncertain system observation data, statistical knowledge of system parameters, expert opinion, and component reliability data in an effort to identify incipient component performance degradations of arbitrary number and magnitude. The technique involves the use of multiple adaptive Kalman filters for fault estimation, the results of which are screened using standard hypothesis testing procedures to define a set of component events that could have transpired. Latin Hypercube sample each of these feasible component events in terms of uncertain component reliability data and filter estimates. The capabilities of the procedure are demonstrated through the analysis of a simulated small magnitude binary component fault in a boiling water reactor balance of plant. The results show that the procedure has the potential to be a very effective tool for incipient component fault diagnosis

  7. 21 CFR 882.5550 - Central nervous system fluid shunt and components.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Central nervous system fluid shunt and components... Central nervous system fluid shunt and components. (a) Identification. A central nervous system fluid... central nervous system to an internal delivery site or an external receptacle for the purpose of relieving...

  8. Component Configuration Control System: an application of logic programming

    International Nuclear Information System (INIS)

    Stratton, R.C.; Town, G.G.

    1985-01-01

    A computer application system is described which provides nuclear reactor power plant operators with an improved decision support system. This system combines traditional computer applications such as graphics display with artificial intelligence methodologies such as reasoning and diagnosis so as to improve plant operability. This paper discusses the issues, and a solution, involved with the system integration of applications developed using traditional and artificial intelligence languages

  9. Dynamic k-out-of-n system reliability with component partnership

    International Nuclear Information System (INIS)

    Coit, David W.; Chatwattanasiri, Nida; Wattanapongsakorn, Naruemon; Konak, Abdullah

    2015-01-01

    This paper describes a new k-out-of-n system reliability model that is appropriate for certain design problems when the minimum number of required components, k, changes dynamically in response to failures to maximize the utility of the available collection of functioning components. This new model shares some distinct similarities with weighted k-out-of-n models and for some problems they produce the same result. However, there are subtle and distinct differences, and in practice, there are some complex applications have not been properly explained or modeled by traditional or extended k-out-of-n system models. For this application, components are arranged in a k-out-of-n configuration of heterogeneous components with different performance levels. Component performance is indicated by a component-specific component partnership level; the fewer partners required to operate successfully implies higher performance. The components can work collectively with partners at the same level to maintain system reliability, or they can create a partnership group with components at higher performance levels which serve as replacements to provide the necessary number of working components. When components fail, the dynamic k-out-of-n configuration maintains reliability of the system with changing k by having components create partnerships with other components at the same level or above. To demonstrate the model, a system replacement maintenance policy based on a replacement interval variable is applied to an example system to obtain the optimal replacement time. - Highlights: • A new k-out-of-n system reliability model is presented. • Components can form partnerships with other components. • The new k-out-of-n model is presented with a dynamic or changing k. • The new model is for systems with components that must work together in a group

  10. The Blame Game: Performance Analysis of Speaker Diarization System Components

    NARCIS (Netherlands)

    Huijbregts, M.A.H.; Wooters, Chuck

    2007-01-01

    In this paper we discuss the performance analysis of a speaker diarization system similar to the system that was submitted by ICSI at the NIST RT06s evaluation benchmark. The analysis that is based on a series of oracle experiments, provides a good understanding of the performance of each system

  11. A Hybrid Hardware and Software Component Architecture for Embedded System Design

    Science.gov (United States)

    Marcondes, Hugo; Fröhlich, Antônio Augusto

    Embedded systems are increasing in complexity, while several metrics such as time-to-market, reliability, safety and performance should be considered during the design of such systems. A component-based design which enables the migration of its components between hardware and software can cope to achieve such metrics. To enable that, we define hybrid hardware and software components as a development artifact that can be deployed by different combinations of hardware and software elements. In this paper, we present an architecture for developing such components in order to construct a repository of components that can migrate between the hardware and software domains to meet the design system requirements.

  12. Modeling of a remote inspection system for NSSS components

    International Nuclear Information System (INIS)

    Choi, Yoo Rark; Kim, Jae Hee; Lee, Jae Cheol

    2003-03-01

    Safety inspection for safety-critical unit of nuclear power plant has been processed using off-line technology. Thus we can not access safety inspection system and inspection data via network such as internet. We are making an on-line control and data access system based on WWW and JAVA technologies which can be used during plant operation to overcome these problems. Users can access inspection systems and inspection data only using web-browser. This report discusses about analysis of the existing remote system and essential techniques such as Web, JAVA, client/server model, and multi-tier model. This report also discusses about a system modeling that we have been developed using these techniques and provides solutions for developing an on-line control and data access system

  13. Development of a remote inspection system for NSSS components

    International Nuclear Information System (INIS)

    Choi, Yoo Rark; Kim, Jae Hee; Lee, Jae Cheol

    2004-03-01

    Different operating systems of computerized inspection equipment cause serious problems in graphic user interface between control computers of inspection equipment and remote user computers. Management cost of interface version is very expensive even if the system has been developed. A solution for the interface problems in accessing the remote inspection system is web-based technology. But time-delay problem of web and java, a compiled type S/W that cooperates with web in control computers of inspection equipment is necessary to solve it. This report describes solutions for developing a remote inspection system based on web and java technology

  14. Complement fixation test to C burnetii

    Science.gov (United States)

    ... complement fixation test; Coxiella burnetii - complement fixation test; C burnetii - complement fixation test ... a specific foreign substance ( antigen ), in this case, C burnetii . Antibodies defend the body against bacteria, viruses, ...

  15. Complement-mediated solubilization of immune complexes and their interaction with complement C3 receptors

    DEFF Research Database (Denmark)

    Petersen, Ivan; Baatrup, Gunnar; Jepsen, H H

    1985-01-01

    Some of the molecular events in the complement (C)-mediated solubilization of immune complexes (IC) have been clarified in recent years. The solubilization is primarily mediated by alternative C pathway proteins whereas factors in the classical pathway accelerate the process. Components of the me......Some of the molecular events in the complement (C)-mediated solubilization of immune complexes (IC) have been clarified in recent years. The solubilization is primarily mediated by alternative C pathway proteins whereas factors in the classical pathway accelerate the process. Components...... of the cellular localization, expression and structure of the C3 receptors, especially the C3b (CR1) receptor, has been considerably extended in the last few years, whereas our understanding of the physiological role of these receptors is still fragmentary. However, it is becoming increasingly evident...

  16. Assessment and management of ageing of major nuclear power plant components important to safety: Metal components of BWR containment systems

    International Nuclear Information System (INIS)

    2000-10-01

    plant components addressed in the reports. This report addresses the metal components of BWR containment systems. The primary ageing mechanisms that may potentially impact the structural capacity, leaktight integrity, or service life of BWR containments are corrosion of metal components and stress corrosion cracking of bellows. Other potential degradation mechanisms include fatigue and mechanical wear. Areas of concern are where surfaces are inaccessible for inspection (e.g areas adjacent to floors, where the containment vessel is embedded in concrete, and locations adjacent to equipment or other structures)

  17. Role of Phosphate Transport System Component PstB1 in Phosphate Internalization by Nostoc punctiforme.

    Science.gov (United States)

    Hudek, L; Premachandra, D; Webster, W A J; Bräu, L

    2016-11-01

    In bacteria, limited phosphate availability promotes the synthesis of active uptake systems, such as the Pst phosphate transport system. To understand the mechanisms that facilitate phosphate accumulation in the cyanobacterium Nostoc punctiforme, phosphate transport systems were identified, revealing a redundancy of Pst phosphate uptake systems that exists across three distinct operons. Four separate PstB system components were identified. pstB1 was determined to be a suitable target for creating phenotypic mutations that could result in the accumulation of excessive levels of phosphate through its overexpression or in a reduction of the capacity to accumulate phosphate through its deletion. Using quantitative real-time PCR (qPCR), it was determined that pstB1 mRNA levels increased significantly over 64 h in cells cultured in 0 mM added phosphate and decreased significantly in cells exposed to high (12.8 mM) phosphate concentrations compared to the level in cells cultured under normal (0.8 mM) conditions. Possible compensation for the loss of PstB1 was observed when pstB2, pstB3, and pstB4 mRNA levels increased, particularly in cells starved of phosphate. The overexpression of pstB1 increased phosphate uptake by N. punctiforme and was shown to functionally complement the loss of PstB in E. coli PstB knockout (PstB - ) mutants. The knockout of pstB1 in N. punctiforme did not have a significant effect on cellular phosphate accumulation or growth for the most part, which is attributed to the compensation for the loss of PstB1 by alterations in the pstB2, pstB3, and pstB4 mRNA levels. This study provides novel in vivo evidence that PstB1 plays a functional role in phosphate uptake in N. punctiforme IMPORTANCE: Cyanobacteria have been evolving over 3.5 billion years and have become highly adept at growing under limiting nutrient levels. Phosphate is crucial for the survival and prosperity of all organisms. In bacteria, limited phosphate availability promotes the

  18. Effect of farming system on colour components of wheat noodles

    Directory of Open Access Journals (Sweden)

    Magdalena Lacko-Bartosova

    2016-07-01

    Full Text Available Colour of noodles is definitely a key element of a consumer's buying decisions. It can be influenced by many factors. Conditions, under which is winter wheat grown, can be considered as one of these factors. The aim of this work was to evaluate colour of noodles that were prepared from winter wheat grown in ecological and integrated arable farming systems, after different forecrops with two levels of fertilization (fertilized and unfertilized during the years 2009, 2010 and 2011. Winter wheat noodles were prepared from white flour and wholegrain flour and its colour was evaluated using the spectro-colorimeter. Colour was measured by three coordinates: lightness L*, red/ green value a* and yellow/ blue value b*. Wholegrain noodles had lower L* value, so they were darker than white flour noodles, with higher redness and higher yellowness. Colour of white flour noodles and wholegrain noodles was significantly influenced by crop nutrition (fertilized and unfertilized variants, farming system and meteorological conditions during experimental years. Wholegrain noodles from ecological system were darker, with lower lightness and higher redness compared to noodles from integrated system. White flour noodles from ecological system were also darker compared to noodles from integrated system. Fertilization decreased lightness of white flour noodles, on the contrary, fertilization increased the lightness and decreased the redness of wholegrain noodles. In non-fertilized treatment, ecological wheat noodles were darker, with higher redness and yellowness than noodles prepared from winter wheat grown in integrated arable farming system.

  19. An overview of the NASA electronic components information management system

    Science.gov (United States)

    Kramer, G.; Waterbury, S.

    1991-01-01

    The NASA Parts Project Office (NPPO) comprehensive data system to support all NASA Electric, Electronic, and Electromechanical (EEE) parts management and technical data requirements is described. A phase delivery approach is adopted, comprising four principal phases. Phases 1 and 2 support Space Station Freedom (SSF) and use a centralized architecture with all data and processing kept on a mainframe computer. Phases 3 and 4 support all NASA centers and projects and implement a distributed system architecture, in which data and processing are shared among networked database servers. The Phase 1 system, which became operational in February of 1990, implements a core set of functions. Phase 2, scheduled for release in 1991, adds functions to the Phase 1 system. Phase 3, to be prototyped beginning in 1991 and delivered in 1992, introduces a distributed system, separate from the Phase 1 and 2 system, with a refined semantic data model. Phase 4 extends the data model and functionality of the Phase 3 system to provide support for the NASA design community, including integration with Computer Aided Design (CAD) environments. Phase 4 is scheduled for prototyping in 1992 to 93 and delivery in 1994.

  20. Routine testing on protective and safety systems and components

    International Nuclear Information System (INIS)

    Rysy, W.

    1977-01-01

    1) In-process inspection, tests during commissioning. 2) Tests during reactor operation. 2.1) Reactor protection system, for example: continuous auto-testing by a dynamic system, check of the output signals; 2.2) safety features: selected examples: functional tests on the ECCS, trial operation of the emergency diesels. 3) Tests during refuelling phase. 3.1) Containment: Leakage rate tests, leak testing; 3.2) coolant system: selected examples: inservice inspections of the pressure vessel, eddy current testing of the steam generator, functional tests of safety valves. (orig./HP) [de

  1. Human as a component of a nuclear material safeguard system

    International Nuclear Information System (INIS)

    Morgan, D.E.; Schechter, R.S.

    1978-01-01

    Many human vigilance experiments are summarized and principles are extracted which should be useful in designing and evaluating a nuclear material safeguard system. A human is a poor observer and is not a dependable part of any man-machine system when required to function as an observer. There are a few techniques which improve his performance by providing feedback. A conceptual model is presented which is helpful in design and evaluation of systems. There is some slight experimental support for the model. Finally, some techniques of time study and statistical control charting will be useful as a means of detecting nuclear diversion attempts

  2. LOGIC SIMULATION OF LIFE SUPPORT SYSTEM COMPONENT IN REAL TIME

    Directory of Open Access Journals (Sweden)

    A. S. Marchenko

    2016-01-01

    Full Text Available Abstract. The article proposed the use of simulation methods for evaluating the effectiveness of a stepped fan engine speed control while maintaining the air flow volume in the set boundaries of the «fan-filter» system. A detailed algorithm of the program made on the basis of an Any Logic software package. Is analyzed the possibility of using the proposed method in the design of ventilation systems.The proposed method allows at the design stage to determine the maximum replacement intervals of the systems filter elements, as well as to predict the time to switch the fan motor speeds. Using of the technique allows to refuse the complex air flow systems and maximize the life of the filter elements set.Methods of logical processes modeling allows to reduce construction costs and improve energy efficiency of buildings. 

  3. Basic components of a national control system for nuclear materials

    International Nuclear Information System (INIS)

    Rabot, G.

    1986-01-01

    The paper presents the different aspects related to the organization and the functioning of a national control and accounting system for nuclear materials. The legal aspects and the relations with the IAEA are included

  4. High performance coated board inspection system based on commercial components

    CERN Document Server

    Barjaktarovic, M; Radunovic, J

    2007-01-01

    This paper presents a vision system for defect (fault) detection on a coated board developed using three industrial firewire cameras and a PC. Application for image processing and system control was realized with the LabView software package. Software for defect detection is based on a variation of the image segmentation algorithm. Standard steps in image segmentation are modified to match the characteristics of defects. Software optimization was accomplished using SIMD (Single Instruction Multiple Data) technology available in the Intel Pentium 4 processors that provided real time inspection capability. System provides benefits such as: improvement in production process, higher quality of delivered coated board and reduction of waste. This was proven during successful exploitation of the system for more than a year.

  5. Type systems for distributed programs components and sessions

    CERN Document Server

    Dardha, Ornela

    2016-01-01

    In this book we develop powerful techniques based on formal methods for the verification of correctness, consistency and safety properties related to dynamic reconfiguration and communication in complex distributed systems. In particular, static analysis techniques based on types and type systems are an adequate methodology considering their success in guaranteeing not only basic safety properties, but also more sophisticated ones like deadlock or lock freedom in concurrent settings. The main contributions of this book are twofold. i) We design a type system for a concurrent object-oriented calculus to statically ensure consistency of dynamic reconfigurations. ii) We define an encoding of the session pi-calculus, which models communication in distributed systems, into the standard typed pi-calculus. We use this encoding to derive properties like type safety and progress in the session pi-calculus by exploiting the corresponding properties in the standard typed pi-calculus.

  6. Force Dynamics of Verb Complementation

    Directory of Open Access Journals (Sweden)

    Jacek Woźny

    2015-12-01

    Full Text Available Force Dynamics of Verb Complementation The concepts of motion and force are both extensively discussed in cognitive linguistics literature. But they are discussed separately. The first usually in the context of ‘motion situations’ (Talmy, Slobin, Zlatev, the other as part of the Force Dynamics framework, which was developed by Talmy. The aim of this paper is twofold: first, to argue that the concepts of force and motion should not be isolated but considered as two inseparable parts of force-motion events. The second goal is to prove that the modified Force Dynamics (force-motion framework can be used for precise characterization of the verb complementation patterns. To this end, a random sample of 50 sentences containing the verb ‘went’ is analyzed, demonstrating the differences between the categories of intensive and intransitive complementation with respect to the linguistically coded parameters of force and motion.

  7. Condition Prediction Model and Component Interaction Fault Tree for Heat Distribution Systems

    National Research Council Canada - National Science Library

    Marsh, Charles

    2001-01-01

    .... Frequent, detailed inspection is largely impractical, and components are subject to complex, obscure interdependencies that can create seemingly unrelated distresses virtually anywhere in the system...

  8. Two-component air heating system. Final report. Zweikomponenten-Luftheizungs-System. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Radtke, W; Thiel, D

    1986-01-01

    The two-component heating system consists of a combination of air-based floor heating and direct air heating, with ventilation and extraction and heat recovery. The direct airflow consists exclusively of heated outside air, the amount corresponding to the building's external air intake requirement. The control system comprises a two-step sequential control of the air throughput of the direct air heating system and of the air distribution for the floor heating airflow. A special heating switch makes it possible to switch off the direct air heating system separately, and to select rapid warm-up. The way in which the new heating system works has been tested in a pilot set-up and proven by comprehensive measurements. In addition, a simulation model was produced which gave substantial confirmation of the measurements. (orig.) With 9 refs., 37 tabs., 63 figs.

  9. System level modeling and component level control of fuel cells

    Science.gov (United States)

    Xue, Xingjian

    This dissertation investigates the fuel cell systems and the related technologies in three aspects: (1) system-level dynamic modeling of both PEM fuel cell (PEMFC) and solid oxide fuel cell (SOFC); (2) condition monitoring scheme development of PEM fuel cell system using model-based statistical method; and (3) strategy and algorithm development of precision control with potential application in energy systems. The dissertation first presents a system level dynamic modeling strategy for PEM fuel cells. It is well known that water plays a critical role in PEM fuel cell operations. It makes the membrane function appropriately and improves the durability. The low temperature operating conditions, however, impose modeling difficulties in characterizing the liquid-vapor two phase change phenomenon, which becomes even more complex under dynamic operating conditions. This dissertation proposes an innovative method to characterize this phenomenon, and builds a comprehensive model for PEM fuel cell at the system level. The model features the complete characterization of multi-physics dynamic coupling effects with the inclusion of dynamic phase change. The model is validated using Ballard stack experimental result from open literature. The system behavior and the internal coupling effects are also investigated using this model under various operating conditions. Anode-supported tubular SOFC is also investigated in the dissertation. While the Nernst potential plays a central role in characterizing the electrochemical performance, the traditional Nernst equation may lead to incorrect analysis results under dynamic operating conditions due to the current reverse flow phenomenon. This dissertation presents a systematic study in this regard to incorporate a modified Nernst potential expression and the heat/mass transfer into the analysis. The model is used to investigate the limitations and optimal results of various operating conditions; it can also be utilized to perform the

  10. What is the existing evidence supporting the efficacy of compression bandage systems containing both elastic and inelastic components (mixed-component systems)? A systematic review.

    Science.gov (United States)

    Welsh, Lynn

    2017-05-01

    To analyse current evidence on the efficacy of bandage systems containing both elastic and inelastic components (mixed-component systems). International consensus on the efficacy of types of compression systems is difficult to achieve; however, mixed-component systems are being promoted as combining the best properties of both elastic and inelastic bandage systems and increasingly being used to treat venous leg ulcers in practice. A systematic literature review. Search terms such as venous leg ulcer, varicose ulcer, leg ulcer, compression, bandage, elastic, inelastic, short stretch, healing rate, interface pressure, mixed component, two-layer, four-layer and multi-layer were used in database and hand searches in several combinations. Limits were set for years 2005-March 2015 and English-language publications. A total of 475 studies were identified at initial search, and following elimination from abstract and title, this was reduced to 7. A further study was identified on Google Scholar, bringing the final number of studies fitting inclusion criteria to 8. The following subgroups relating to outcomes of efficacy were identified: ulcer healing, maintenance of interface pressure, slippage, ease of application and patient quality of life. Mixed-component systems were found to have comparable ulcer healing rates to alternative compression systems and be easy to apply; have similar abilities to maintain pressure as four-layer bandages and better abilities than short-stretch bandages; have less slippage than alternative systems; and to be significantly associated with several favourable quality of life outcomes. Clinician skill in bandage application was an uncontrolled variable in all eight papers included in the review, which may limit reliability of findings. This review synthesises existing evidence on the efficacy of mixed-component systems and encourages clinicians to regard them as an effective alternative to purely elastic or inelastic compression systems

  11. Component state-based integrated importance measure for multi-state systems

    International Nuclear Information System (INIS)

    Si, Shubin; Levitin, Gregory; Dui, Hongyan; Sun, Shudong

    2013-01-01

    Importance measures in reliability engineering are used to identify weak components and/or states in contributing to the reliable functioning of a system. Traditionally, importance measures do not consider the possible effect of groups of transition rates among different component states, which, however, has great effect on the component probability distribution and should therefore be taken into consideration. This paper extends the integrated importance measure (IIM) to estimate the effect of a component residing at certain states on the performance of the entire multi-state systems. This generalization of IIM describes in which state it is most worthy to keep the component to provide the desired level of system performance, and which component is the most important to keep in some state and above for improving the performance of the system. An application to an oil transportation system is presented to illustrate the use of the suggested importance measure

  12. Qualification of C and I systems using new technology components

    International Nuclear Information System (INIS)

    Gossner, S.

    1986-01-01

    Whereas one may find an almost complete change-over to microprocessor based automation and supervision systems in numerous industrial fields, a similar development did not yet take place in C and I systems of West German nuclear power plants. Neglecting some unimportant exceptions C and I systems of West German nuclear power plants are fully conventional. This situation, however, will not last forever. There is a growing understanding that by means of microcomputer-based C and I equipment it will be possible: to solve many problems more exactly, e.g. calculations of reactor power, power distribution, water levels, DNBR; to reduce the number of detailed informations and alarms concentrated in control rooms and control stations and to condense these informations into easily to understand pictorial displays, e.g. surveillance or trend pictures on graphic CRT-display units; to reduce cabling amount by using sequential data transmission, e.g. by means of bus-systems. It's clear in the near future microprocessors will enter not only operational C and I equipment but C and I systems important to safety as well. Therefore it's high time to analyse existing design and qualification requirements, qualification methods and qualification procedures whether or not to be suitable and sufficient

  13. Complement: Alive and Kicking Nanomedicines

    DEFF Research Database (Denmark)

    Andersen, Alina Joukainen; Hashemi, S.H.; Andresen, Thomas Lars

    2009-01-01

    Administration of liposome- and polymer-based clinical nanomedicines, as well as many other proposed multifunctional nanoparticles, often triggers hypersensitivity reactions without the involvement of IgE. These anaphylactic reactions are believed to be secondary to activation of the complement...... their procoagulant activity, and has the capacity to elicit non-lytic stimulatory responses from vascular endothelial cells. Here we discuss the molecular basis of complement activation by liposomes, including poly(ethylene glycol) coated vesicles, and other related lipid-based and phospholipid-poly(ethylene glycol...

  14. Critical Components in Microfluidic Systems for Drug Delivery

    DEFF Research Database (Denmark)

    Bitsch, Lennart

    2006-01-01

    Formålet med denne afhandling har været at evaluere mulighederne for at anvende mikrofluide systemer til medicinsk behandling. Vi har især fokuseret på sikkerhed i reciprokerende pumper, med henblik på en kontinuer insulin behandling, og har identificeret mikroventiler som værende kritiske......-mikroventil. Sammenlignet med systemer der benytter passive ventiler opnår vi et markant højere sikkerhedsniveau med en drejeventil. Teknologien bygger på konstruktion med bløde inkompressible gummimaterialer, og hårde overflader. Grundlæggende studier af dynamisk friktion mellem gummi og en hård overflade viser...... dreje-mikroventiler med gode pakninsgsegenskaber. De overvejende fordele ved nedskalering af mekaniske systemer er mere kompakte, lavenergi-apparater. Vi har vist muligheden for at lave dreje-mikroventiler med lavt energiforbrug og gode pakningsegenskaber ved konstruktion og test af en demonstrator med...

  15. Potential drop crack measurement systems for CANDU components

    Energy Technology Data Exchange (ETDEWEB)

    Sahney, R [Carleton Univ., Ottawa, ON (Canada)

    1994-12-31

    A project to develop an automated crack measurement system for CANDU pressure tube burst testing is currently underway. The system will utilize either Direct Current Potential Drop (DCPD) or Alternating Current Potential Drop (ACPD) techniques for crack measurement. The preliminary stage of the project involves testing and comparison of both ACPD and DCPD methods on a Zr - 2.5% Nb alloy plate with saw cuts (used to simulate cracks). Preliminary results show that both ACPD and DCPD techniques are capable of detecting cracks; further testing is in progress to determine the ability of each of the two systems to make accurate crack depth measurements. This paper will describe the two potential drop techniques and will present test results from the experimental program. (author). 10 refs., 7 figs.

  16. Component-based modeling of systems for automated fault tree generation

    International Nuclear Information System (INIS)

    Majdara, Aref; Wakabayashi, Toshio

    2009-01-01

    One of the challenges in the field of automated fault tree construction is to find an efficient modeling approach that can support modeling of different types of systems without ignoring any necessary details. In this paper, we are going to represent a new system of modeling approach for computer-aided fault tree generation. In this method, every system model is composed of some components and different types of flows propagating through them. Each component has a function table that describes its input-output relations. For the components having different operational states, there is also a state transition table. Each component can communicate with other components in the system only through its inputs and outputs. A trace-back algorithm is proposed that can be applied to the system model to generate the required fault trees. The system modeling approach and the fault tree construction algorithm are applied to a fire sprinkler system and the results are presented

  17. FDIR for the IMU Component of AOCS Systems

    Directory of Open Access Journals (Sweden)

    Maurício N. Pontuschka

    2014-01-01

    Full Text Available The main objective of this paper is the study of a FDIR for an IMU aiming at space applications with focus on the gyro signal analysis and the tests of the filtering algorithms. The algorithms have been tested by using lab data provided by the DMC LABSIM (Physical’s Simulation Laboratory of the Space Mechanics and Control Division of INPE. The results have demonstrated good agreement with the concepts applied in this study. Automatic detection procedures are very important in the characterization of occurrence, definition of criteria, and device types in the scenario of AOCS FDIR. An IMU comprised of four gyros in a tetrahedral configuration is one of the assumed components for the AOCS (attitude and orbit control subsystem considered in this work. The types of failures considered in this paper are the step abrupt change, ramp/drift/slow, stuck, cyclic, erratic, spike, and finally the stuck for variance alteration noise. An appropriate algorithm for the automatic detection of each type of fault is developed. The approach includes the mapping capability of fault event indicators to the IMU. This mapping is very important in the characterization of the occurrence, definition of criteria, and device types as well as associated fault identification for an AOCS.

  18. Computers as Components Principles of Embedded Computing System Design

    CERN Document Server

    Wolf, Wayne

    2008-01-01

    This book was the first to bring essential knowledge on embedded systems technology and techniques under a single cover. This second edition has been updated to the state-of-the-art by reworking and expanding performance analysis with more examples and exercises, and coverage of electronic systems now focuses on the latest applications. Researchers, students, and savvy professionals schooled in hardware or software design, will value Wayne Wolf's integrated engineering design approach.The second edition gives a more comprehensive view of multiprocessors including VLIW and superscalar archite

  19. Conference on Radiation and its Effects on Components and Systems

    CERN Document Server

    2017-01-01

    The aim of RADECS conferences is to provide an annual European forum for the presentation and discussion of the latest advances in the field of radiation effects on electronic and photonic materials, devices, circuits, sensors, and systems. The scope of the conference encompasses technological processes and design techniques for producing radiation tolerant systems for space, aeronautical or terrestrial applications, as well as relevant methodologies for their characterization and qualification. The conference features a technical program, an Industrial Exhibit, and one day tutorial or ‘short course’ on radiation effects. The technical program includes oral and poster sessions and round tables.

  20. Complement Attack against Aspergillus and Corresponding Evasion Mechanisms

    Directory of Open Access Journals (Sweden)

    Cornelia Speth

    2012-01-01

    Full Text Available Invasive aspergillosis shows a high mortality rate particularly in immunocompromised patients. Perpetually increasing numbers of affected patients highlight the importance of a clearer understanding of interactions between innate immunity and fungi. Innate immunity is considered to be the most significant host defence against invasive fungal infections. Complement represents a crucial part of this first line defence and comprises direct effects against invading pathogens as well as bridging functions to other parts of the immune network. However, despite the potency of complement to attack foreign pathogens, the prevalence of invasive fungal infections is increasing. Two possible reasons may explain that phenomenon: First, complement activation might be insufficient for an effective antifungal defence in risk patients (due to, e.g., low complement levels, poor recognition of fungal surface, or missing interplay with other immune elements in immunocompromised patients. On the other hand, fungi may have developed evasion strategies to avoid recognition and/or eradication by complement. In this review, we summarize the most important interactions between Aspergillus and the complement system. We describe the various ways of complement activation by Aspergillus and the antifungal effects of the system, and also show proven and probable mechanisms of Aspergillus for complement evasion.

  1. In silico dissection of Type VII Secretion System components across ...

    Indian Academy of Sciences (India)

    2016-02-12

    Feb 12, 2016 ... Bio-Sciences R&D Division, TCS Innovation Labs, Tata Research Development & Design Centre, ... Type VII Secretion System (T7SS) is one of the factors involved in virulence of ... Such wide distribution of T7SS and.

  2. Photovoltaic system and components price development in the Netherlands

    NARCIS (Netherlands)

    van Sark, W.G.J.H.M.; Schoen, Tony

    2017-01-01

    Market inventories have been made since fall 2011 for modules, inverters, and systems that are available for purchase in the Netherlands to provide the fast growing Dutch market with proper market information. The most recent inventory was made in December 2016. After the drop in module price in

  3. Component Based System Framework for Dynamic B2B Interaction

    NARCIS (Netherlands)

    Hu jinmin, Jinmin; Grefen, P.W.P.J.

    Business-to-Business (B2B) collaboration is becoming a pivotal way to bring today's enterprises to success in the dynamically changing e-business environment. Though many business-to-business protocols are developed to support B2B interaction, none are generally accepted. A B2B system should support

  4. 14 CFR 33.91 - Engine system and component tests.

    Science.gov (United States)

    2010-01-01

    ... AIRWORTHINESS STANDARDS: AIRCRAFT ENGINES Block Tests; Turbine Aircraft Engines § 33.91 Engine system and..., reliability, and durability. (c) Each unpressurized hydraulic fluid tank may not fail or leak when subjected... hydraulic fluid tank must meet the requirements of § 33.64. (d) For an engine type certificated for use in...

  5. A computer-controlled electronic system for the ultrasonic NDT of components for nuclear power stations

    International Nuclear Information System (INIS)

    Rehrmann, M.; Harbecke, D.

    1987-01-01

    The paper describes an automatic ultrasonic testing system combined with a computer-controlled electronics system, called IMPULS I, for the non-destructive testing of components of nuclear reactors. The system can be used for both in-service inspection and for inspection during the manufacturing process. IMPUL I has more functions and less components than conventional ultrasonic systems, and the system gives good reproducible test results and is easy to operate. (U.K.)

  6. PV System Component Fault and Failure Compilation and Analysis.

    Energy Technology Data Exchange (ETDEWEB)

    Klise, Geoffrey Taylor; Lavrova, Olga; Gooding, Renee Lynne

    2018-02-01

    This report describes data collection and analysis of solar photovoltaic (PV) equipment events, which consist of faults and fa ilures that occur during the normal operation of a distributed PV system or PV power plant. We present summary statistics from locations w here maintenance data is being collected at various intervals, as well as reliability statistics gathered from that da ta, consisting of fault/failure distributions and repair distributions for a wide range of PV equipment types.

  7. Updated optical read/write memory system components

    Science.gov (United States)

    1974-01-01

    A survey of the building blocks of the electro-optic read/write system was made. Critical areas and alternate paths are discussed. The latest PLZT block data composer is analyzed. Stricter controls in the production and fabrication of PLZT are implied by the performance of the BDC. A reverse charge before erase has eliminated several problems observed in the parallel plane charging process for photoconductor-thermoplastic hologram storage.

  8. ACCOUNTING, AN ESSENTIAL COMPONENT OF THE INFORMATION SYSTEM

    Directory of Open Access Journals (Sweden)

    PALIU -POPA LUCIA

    2013-02-01

    Full Text Available In the context of deep financial and economic transformations taking place both nationally and globally, theneed and appropriateness of ongoing and increased involvement of accounting information in the management processis increasingly obvious under the increasing credibility and relevance of such information in user perception. Althoughthe data, information and knowledge provided by accounting are not the only ones characterizing a particular economicunit, we believe they are an essential element for analysis and assessment of the entity's statement of assets, motivationaccording to which accounting is seen by the management, and not only, as the core of the information system.Thus, the accounting information experiences a wide revaluation on all the decision-making stages specific toa field of activity, materialized, for this purpose, in economic and financial indicators that are obtained either directlyfrom synthetic and analytical accounts, or through calculations based on them, regularly and periodically, dependingon the need and possibility of operational knowledge of such indicators or only at certain times.Therefore the main purpose of the accounting information system is to provide each user, according to itsresponsibilities and tasks, with all the necessary information, representing both an interface between the operating andthe management system of the entity, but also an element connecting the internal and the external environment of thecompany, a perspective that motivates our scientific approach orientation in the research of accounting informationand its role in the decision making process.

  9. Deep Charging Evaluation of Satellite Power and Communication System Components

    Science.gov (United States)

    Schneider, T. A.; Vaughn, J. A.; Chu, B.; Wong, F.; Gardiner, G.; Wright, K. H.; Phillips, B.

    2016-01-01

    Deep charging, in contrast to surface charging, focuses on electron penetration deep into insulating materials applied over conductors. A classic example of this scenario is an insulated wire. Deep charging can pose a threat to material integrity, and to sensitive electronics, when it gives rise to an electrostatic discharge or arc. With the advent of Electric Orbit Raising, which requires spiraling through Earth's radiation belts, satellites are subjected to high energy electron environments which they normally would not encounter. Beyond Earth orbit, missions to Jupiter and Saturn face deep charging concerns due to the high energy radiation environments. While predictions can be made about charging in insulating materials, it is difficult to extend those predictions to complicated geometries, such as the case of an insulating coating around a small wire, or a non-uniform silicone grouting on a bus bar. Therefore, to conclusively determine the susceptibility of a system to arcs from deep charging, experimental investigations must be carried out. This paper will describe the evaluation carried out by NASA's Marshall Space Flight Center on subscale flight-like samples developed by Space Systems/Loral, LLC. Specifically, deep charging evaluations of solar array wire coupons, a photovoltaic cell coupon, and a coaxial microwave transmission cable, will be discussed. The results of each evaluation will be benchmarked against control sample tests, as well as typical power system levels, to show no significant deep charging threat existed for this set of samples under the conditions tested.

  10. Novel Two-Component System of Streptococcus sanguinis Affecting Functions Associated with Viability in Saliva and Biofilm Formation.

    Science.gov (United States)

    Camargo, Tarsila M; Stipp, Rafael N; Alves, Lívia A; Harth-Chu, Erika N; Höfling, José F; Mattos-Graner, Renata O

    2018-04-01

    Streptococcus sanguinis is a pioneer species of teeth and a common opportunistic pathogen of infective endocarditis. In this study, we identified a two-component system, S. sanguinis SptRS (SptRS Ss ), affecting S. sanguinis survival in saliva and biofilm formation. Isogenic mutants of sptR Ss (SKsptR) and sptS Ss (SKsptS) showed reduced cell counts in ex vivo assays of viability in saliva compared to those of parent strain SK36 and complemented mutants. Reduced counts of the mutants in saliva were associated with reduced growth rates in nutrient-poor medium (RPMI) and increased susceptibility to the deposition of C3b and the membrane attach complex (MAC) of the complement system, a defense component of saliva and serum. Conversely, sptR Ss and sptS Ss mutants showed increased biofilm formation associated with higher levels of production of H 2 O 2 and extracellular DNA. Reverse transcription-quantitative PCR (RT-qPCR) comparisons of strains indicated a global role of SptRS Ss in repressing genes for H 2 O 2 production (2.5- to 15-fold upregulation of spxB , spxR , vicR , tpk , and ackA in sptR Ss and sptS Ss mutants), biofilm formation, and/or evasion of host immunity (2.1- to 11.4-fold upregulation of srtA , pcsB , cwdP , iga , and nt5e ). Compatible with the homology of SptR Ss with AraC-type regulators, duplicate to multiple conserved repeats were identified in 1,000-bp regulatory regions of downstream genes, suggesting that SptR Ss regulates transcription by DNA looping. Significant transcriptional changes in the regulatory genes vicR , spxR , comE , comX , and mecA in the sptR Ss and sptS Ss mutants further indicated that SptRS Ss is part of a regulatory network that coordinates cell wall homeostasis, H 2 O 2 production, and competence. This study reveals that SptRS Ss is involved in the regulation of crucial functions for S. sanguinis persistence in the oral cavity. Copyright © 2018 American Society for Microbiology.

  11. Optical components of adaptive systems for improving laser beam quality

    Science.gov (United States)

    Malakhov, Yuri I.; Atuchin, Victor V.; Kudryashov, Aleksis V.; Starikov, Fedor A.

    2008-10-01

    The short overview is given of optical equipment developed within the ISTC activity for adaptive systems of new generation allowing for correction of high-power laser beams carrying optical vortices onto the phase surface. They are the kinoform many-level optical elements of new generation, namely, special spiral phase plates and ordered rasters of microlenses, i.e. lenslet arrays, as well as the wide-aperture Hartmann-Shack sensors and bimorph deformable piezoceramics- based mirrors with various grids of control elements.

  12. Handoff algorithm for mobile satellite systems with ancillary terrestrial component

    KAUST Repository

    Sadek, Mirette

    2012-06-01

    This paper presents a locally optimal handoff algorithm for integrated satellite/ground communication systems. We derive the handoff decision function and present the results in the form of tradeoff curves between the number of handoffs and the number of link degradation events in a given distance covered by the mobile user. This is a practical receiver-controlled handoff algorithm that optimizes the handoff process from a user perspective based on the received signal strength rather than from a network perspective. © 2012 IEEE.

  13. TASKA-M exhaust system and its main components

    International Nuclear Information System (INIS)

    Kleefeldt, K.W.; Mueller, R.A.; Schramm, K.

    1985-01-01

    TASKA-M is a study for a mirror based D-T plasma device for fusion technology tests. Mature technology was applied whereever possible. The axial confinement time is relatively short, resulting in a large gas throughput compared to the fusion power level of 6.8 MW. The technological requirements of the exhaust system will not cause undue development problems in either of the two major areas: highly loaded dumps for the conversion of the escaping particle and plasma streams to thermal gas; vacuum pumping facilities. (orig.)

  14. HTS thin films: Passive microwave components and systems integration issues

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, F.A.; Chorey, C.M.; Bhasin, K.B. [National Aeronautics and Space Administration, Cleveland, OH (United States)

    1994-12-31

    The excellent microwave properties of the High-Temperature-Superconductors (HTS) have been amply demonstrated in the laboratory by techniques such as resonant cavity, power transmission and microstrip resonator measurements. The low loss and high Q passive structures made possible with HTS, present attractive options for applications in commercial, military and space-based systems. However, to readily insert HTS into these systems improvement is needed in such areas as repeatability in the deposition and processing of the HTS films, metal-contact formation, wire bonding, and overall film endurance to fabrication and assembly procedures. In this paper we present data compiled in our lab which illustrate many of the problems associated with these issues. Much of this data were obtained in the production of a space qualified hybrid receiver-downconverter module for the Naval Research Laboratory`s High Temperature Superconductivity Space Experiment II (HTSSE-II). Examples of variations observed in starting films and finished circuits will be presented. It is shown that under identical processing the properties of the HTS films can degrade to varying extents. Finally, we present data on ohmic contacts and factors affecting their adhesion to HTS films, strength of wire bonds made to such contacts, and aging effects.

  15. HTS thin films: Passive microwave components and systems integration issues

    International Nuclear Information System (INIS)

    Miranda, F.A.; Chorey, C.M.; Bhasin, K.B.

    1994-01-01

    The excellent microwave properties of the High-Temperature-Superconductors (HTS) have been amply demonstrated in the laboratory by techniques such as resonant cavity, power transmission and microstrip resonator measurements. The low loss and high Q passive structures made possible with HTS, present attractive options for applications in commercial, military and space-based systems. However, to readily insert HTS into these systems improvement is needed in such areas as repeatability in the deposition and processing of the HTS films, metal-contact formation, wire bonding, and overall film endurance to fabrication and assembly procedures. In this paper we present data compiled in our lab which illustrate many of the problems associated with these issues. Much of this data were obtained in the production of a space qualified hybrid receiver-downconverter module for the Naval Research Laboratory's High Temperature Superconductivity Space Experiment II (HTSSE-II). Examples of variations observed in starting films and finished circuits will be presented. It is shown that under identical processing the properties of the HTS films can degrade to varying extents. Finally, we present data on ohmic contacts and factors affecting their adhesion to HTS films, strength of wire bonds made to such contacts, and aging effects

  16. Novel Evasion Mechanisms of the Classical Complement Pathway.

    Science.gov (United States)

    Garcia, Brandon L; Zwarthoff, Seline A; Rooijakkers, Suzan H M; Geisbrecht, Brian V

    2016-09-15

    Complement is a network of soluble and cell surface-associated proteins that gives rise to a self-amplifying, yet tightly regulated system with fundamental roles in immune surveillance and clearance. Complement becomes activated on the surface of nonself cells by one of three initiating mechanisms known as the classical, lectin, and alternative pathways. Evasion of complement function is a hallmark of invasive pathogens and hematophagous organisms. Although many complement-inhibition strategies hinge on hijacking activities of endogenous complement regulatory proteins, an increasing number of uniquely evolved evasion molecules have been discovered over the past decade. In this review, we focus on several recent investigations that revealed mechanistically distinct inhibitors of the classical pathway. Because the classical pathway is an important and specific mediator of various autoimmune and inflammatory disorders, in-depth knowledge of novel evasion mechanisms could direct future development of therapeutic anti-inflammatory molecules. Copyright © 2016 by The American Association of Immunologists, Inc.

  17. 76 FR 69284 - Certain Integrated Solar Power Systems and Components Thereof: Notice of Institution of...

    Science.gov (United States)

    2011-11-08

    ... the United States after importation of certain integrated solar power systems and components thereof... certain integrated solar power systems and components thereof that infringe one or more of claims 6 and 10... INTERNATIONAL TRADE COMMISSION [Investigation No. 337-TA-811] Certain Integrated Solar Power...

  18. 77 FR 39736 - Certain Integrated Solar Power Systems and Components Thereof; Notice of Termination of the...

    Science.gov (United States)

    2012-07-05

    ... certain integrated solar power systems and components thereof by reason of infringement of certain claims... INTERNATIONAL TRADE COMMISSION [Investigation No. 337-TA-811] Certain Integrated Solar Power Systems and Components Thereof; Notice of Termination of the Investigation Based on Settlement AGENCY: U.S...

  19. Two-Component Systems of Mycobacterium tuberculosis—Structure-Based Approaches

    DEFF Research Database (Denmark)

    Tucker, Paul; Nowak, Elzbieta; Morth, Jens Preben

    2007-01-01

    Mycobacterium tuberculosis contains few two‐component systems compared to many other bacteria, possibly because it has more serine/threonine signaling pathways. Even so, these two‐component systems appear to play an important role in early intracellular survival of the pathogen as well as in aspe...

  20. 77 FR 58579 - Certain Two-Way Global Satellite Communication Devices, System and Components Thereof...

    Science.gov (United States)

    2012-09-21

    ... Communication Devices, System and Components Thereof; Institution of Investigation Pursuant to 19 U.S.C. 1337... certain two-way global satellite communication devices, system and components thereof that infringe one or... within the United States after importation of certain two-way global satellite communication devices...

  1. Components for monolithic fiber chirped pulse amplification laser systems

    Science.gov (United States)

    Swan, Michael Craig

    The first portion of this work develops techniques for generating femtosecond-pulses from conventional fabry-perot laser diodes using nonlinear-spectral-broadening techniques in Yb-doped positive dispersion fiber ampliers. The approach employed an injection-locked fabry-perot laser diode followed by two stages of nonlinear-spectral-broadening to generate sub-200fs pulses. This thesis demonstrated that a 60ps gain-switched fabry-perot laser-diode can be injection-locked to generate a single-longitudinal-mode pulse and compressed by nonlinear spectral broadening to 4ps. Two problems have been identified that must be resolved before moving forward with this approach. First, gain-switched pulses from a standard diode-laser have a number of characteristics not well suited for producing clean self-phase-modulation-broadened pulses, such as an asymmetric temporal shape, which has a long pulse tail. Second, though parabolic pulse formation occurs for any arbitrary temporal input pulse profile, deviation from the optimum parabolic input results in extensively spectrally modulated self-phase-modulation-broadened pulses. In conclusion, the approach of generating self-phase-modulation-broadened pulses from pulsed laser diodes has to be modified from the initial approach explored in this thesis. The first Yb-doped chirally-coupled-core ber based systems are demonstrated and characterized in the second portion of this work. Robust single-mode performance independent of excitation or any other external mode management techniques have been demonstrated in Yb-doped chirally-coupled-core fibers. Gain and power efficiency characteristics are not compromised in any way in this novel fiber structure up to the 87W maximum power achieved. Both the small signal gain at 1064nm of 30.3dB, and the wavelength dependence of the small signal gain were comparable to currently deployed large-mode-area-fiber technology. The efficiencies of the laser and amplifier were measured to be 75% and 54

  2. Floodplains: the forgotten and abused component of the fluvial system

    Directory of Open Access Journals (Sweden)

    Heritage George

    2016-01-01

    Full Text Available River restoration is strongly focussed on in-channel initiatives driven by fisheries interests and a continued desire for river stability. This contrasts greatly with the inherently mobile nature of watercourses. What is often overlooked is the fact that many rivers have developed floodplain units that would naturally operate as integrated functional systems, moderating the effects of extreme floods by distributing flow energy and sediment transport capacity through out of bank flooding. Floodplain utilisation for farming activities and landowner intransigence when it comes to acknowledging that the floodplain is part of the river system, has resulted in floodplains being the most degraded fluvial morphologic unit, both in terms of loss of form and function and sheer levels of spatial impact. The degradation has been facilitated by the failure of regulatory mechanisms to adequately acknowledge floodplain form and function. This is testament to the ‘inward looking’ thinking behind national assessment strategies. This paper reviews the state of floodplain systems drawing on quantitative data from England and Wales to argue for greater consideration of the floodplain in relation to river management. The database is poor and must be improved, however it does reveal significant loss of watercourse-floodplain connectivity linked to direct flood alleviation measures and also to altered flood frequency as a result of river downcutting following river engineering. These latter effects have persisted along many watercourses despite the historic nature of the engineering interventions and will continue to exacerbate the risk of flooding to downstream communities. We also present several examples of the local and wider values of reinstating floodplain form and function, demonstrating major ecological gains, improvement to downstream flood reduction, elevation of water quality status and reductions in overall fine sediment loss from farmland. A re

  3. Non-periodic inspection optimization of multi-component and k-out-of-m systems

    International Nuclear Information System (INIS)

    Hajipour, Yassin; Taghipour, Sharareh

    2016-01-01

    This paper proposes a model to find the optimal non-periodic inspection interval over a finite planning horizon for two types of multi-component repairable systems. The first system contains hard-type and soft-type components, and the second system is a k-out-of-m system with m identical components. The failures of components in both systems follow a non-homogeneous Poisson process. A component can be a single part such as battery or line cord, or a subsystem, such as circuit breaker or charger in an infusion pump, which depending on their failures could be either replaced or minimally repaired according to their ages at failure. The systems are inspected at scheduled inspections or when an event of opportunistic inspection or a system failure occur. We develop a model to find the optimal inspection scheme for each system, which results in the minimum total expected cost over the system's lifecycle. We first develop a simulation model to obtain the total expected cost for a given non-periodic inspection scheme, and then integrate the simulation model with a genetic algorithm to obtain the optimal scheme more efficiently. - Highlights: • Non-periodic inspection optimization of two complex systems. • One system consists of soft-type and hard-type components. • The second system is a k-out-of-m system. • Integration of a simulation model and the genetic algorithm. • The model can be used when inspection is challenging or costly.

  4. Deep Charging Evaluation of Satellite Power and Communication System Components

    Science.gov (United States)

    Schneider, T. A.; Vaughn, J. A.; Chu, B.; Wong, F.; Gardiner, G.; Wright, K. H.; Phillips, B.

    2016-01-01

    A set of deep charging tests has been carried out by NASA's Marshall Space Flight Center on subscale flight-like samples developed by Space Systems/Loral, LLC. The samples, which included solar array wire coupons, a photovoltaic cell coupon, and a coaxial microwave transmission cable, were placed in passive and active (powered) circuit configurations and exposed to electron radiation. The energy of the electron radiation was chosen to deeply penetrate insulating (dielectric) materials on each sample. Each circuit configuration was monitored to determine if potentially damaging electrostatic discharge events (arcs) were developed on the coupon as a result of deep charging. The motivation for the test, along with charging levels, experimental setup, sample details, and results will be discussed.

  5. Optical components and systems for synchrotron radiation: an introduction

    International Nuclear Information System (INIS)

    Howells, M.R.

    1981-01-01

    A brief description of the nature and origins of synchrotron radiation is given with special reference to its geometrical optical properties and the use of storage rings as light souces. The geographical distribution of SR sources in the world is reviewed and some discussion of the level of experimental activity is given. Estimates of future levels of experimental activity are also made both for existing storage rings and those planned for the future. Calculations of the approximate number of mirrors and gratings that will be required are offered. Some general considerations are outlined showing how synchrotron radiation optical systems couple to the light source and indicating which parameters need to be maximized for best overall performance

  6. Complement evasion by Bordetella pertussis: implications for improving current vaccines.

    Science.gov (United States)

    Jongerius, Ilse; Schuijt, Tim J; Mooi, Frits R; Pinelli, Elena

    2015-04-01

    Bordetella pertussis causes whooping cough or pertussis, a highly contagious disease of the respiratory tract. Despite high vaccination coverage, reported cases of pertussis are rising worldwide and it has become clear that the current vaccines must be improved. In addition to the well-known protective role of antibodies and T cells during B. pertussis infection, innate immune responses such as the complement system play an essential role in B. pertussis killing. In order to evade this complement activation and colonize the human host, B. pertussis expresses several molecules that inhibit complement activation. Interestingly, one of the known complement evasion proteins, autotransporter Vag8, is highly expressed in the recently emerged B. pertussis isolates. Here, we describe the current knowledge on how B. pertussis evades complement-mediated killing. In addition, we compare this to complement evasion strategies used by other bacterial species. Finally, we discuss the consequences of complement evasion by B. pertussis on adaptive immunity and how identification of the bacterial molecules and the mechanisms involved in complement evasion might help improve pertussis vaccines.

  7. Inactivation of complement by Loxosceles reclusa spider venom.

    Science.gov (United States)

    Gebel, H M; Finke, J H; Elgert, K D; Cambell, B J; Barrett, J T

    1979-07-01

    Zymosan depletion of serum complement in guinea pigs rendered them highly resistant to lesion by Loxosceles reclusa spider venom. Guinea pigs deficient in C4 of the complement system are as sensitive to the venom as normal guinea pigs. The injection of 35 micrograms of whole recluse venom intradermally into guinea pigs lowered their complement level by 35.7%. Brown recluse spider venom in concentrations as slight as 0.02 micrograms protein/ml can totally inactivate one CH50 of guinea pig complement in vitro. Bee, scorpion, and other spider venoms had no influence on the hemolytic titer of complement. Fractionation of recluse spider venom by Sephadex G-200 filtration separated the complement-inactivating property of the venom into three major regions which could be distinguished on the basis of heat stability as well as size. None was neutralized by antivenom. Polyacrylamide gel electrophoresis of venom resolved the complement inactivators into five fractions. Complement inactivated by whole venom or the Sephadex fractions could be restored to hemolytic activity by supplements of fresh serum but not by heat-inactivated serum, pure C3, pure C5, or C3 and C5 in combination.

  8. Lower-Energy Energy Storage System (LEESS) Component Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Gonder, J.; Cosgrove, J.; Shi, Y.; Saxon, A.; Pesaran, A.

    2014-10-01

    Alternate hybrid electric vehicle (HEV) energy storage systems (ESS) such as lithium-ion capacitors (LICs) and electrochemical double-layer capacitor (EDLC) modules have the potential for improved life, superior cold temperature performance, and lower long-term cost projections relative to traditional battery storage systems. If such lower-energy ESS (LEESS) devices can also be shown to maintain high HEV fuel savings, future HEVs designed with these devices could have an increased value proposition relative to conventional vehicles. NREL's vehicle test platform is helping validate the in-vehicle performance capability of alternative LEESS devices and identify unforeseen issues. NREL created the Ford Fusion Hybrid test platform for in-vehicle evaluation of such alternative LEESS devices, bench testing of the initial LIC pack, integration and testing of the LIC pack in the test vehicle, and bench testing and installation of an EDLC module pack. EDLC pack testing will continue in FY15. The in-vehicle LIC testing results suggest technical viability of LEESS devices to support HEV operation. Several LIC configurations tested demonstrated equivalent fuel economy and acceleration performance as the production nickel-metal-hydride ESS configuration across all tests conducted. The lowest energy LIC scenario demonstrated equivalent performance over several tests, although slightly higher fuel consumption on the US06 cycle and slightly slower acceleration performance. More extensive vehicle-level calibration may be able to reduce or eliminate these performance differences. The overall results indicate that as long as critical attributes such as engine start under worst case conditions can be retained, considerable ESS downsizing may minimally impact HEV fuel savings.

  9. Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formation

    Directory of Open Access Journals (Sweden)

    Yu-Tze Horng

    2018-04-01

    Full Text Available Background/Purpose: Klebsiella pneumoniae is one of the leading causes of device-related infections (DRIs, which are associated with attachment of bacteria to these devices to form a biofilm. The latter is composed of not only bacteria but also extracellular polymeric substances (EPSes consisting of extracellular DNAs, polysaccharides, and other macromolecules. The phosphoenolpyruvate (PEP:carbohydrate phosphotransferase system (PTS regulates diverse processes of bacterial physiology. In the genome of K. pneumoniae MGH 78578, we found an uncharacterized enzyme II complex homolog of PTS: KPN00353 (EIIA homolog, KPN00352 (EIIB homolog, and KPN00351 (EIIC homolog. The aim of this study was to characterize the potential physiological role of KPN00353, KPN00352, and KPN00351 in biofilm formation by K. pneumoniae. Methods/Results: We constructed the PTS mutants and recombinant strains carrying the gene(s of PTS. The recombinant K. pneumoniae strain overexpressing KPN00353–KPN00352–KPN00351 produced more extracellular matrix than did the vector control according to transmission and scanning electron microscopy. Judging by quantification of biofilm formation, of extracellular DNA (eDNA, and of capsular polysaccharide, the recombinant strain overexpressing KPN00353-KPN00352-KPN00351 produced more biofilm and capsular polysaccharide after overnight culture and more eDNA in the log phase as compared to the vector control. Conclusion: The genes, KPN00353–KPN00352–KPN00351, encode a putative enzyme II complex in PTS and positively regulate biofilm formation by enhancing production of eDNA and capsular polysaccharide in K. pneumoniae. Five proteins related to chaperones, to the citric acid cycle, and to quorum sensing are upregulated by the KPN00353–KPN00352–KPN00351 system. Keywords: Klebsiella, PTS, Biofilm, eDNA, Polysaccharide

  10. Components and systems for hybrid- and electromobiles; Komponenten und Systeme fuer Hybrid- und Elektrofahrzeuge

    Energy Technology Data Exchange (ETDEWEB)

    Immle, Michael; Burgmayr, Thomas [Panasonic Electric Works Europe AG, Holzkirchen (Germany)

    2010-07-01

    On the Hybrid and Electric Vehicle sector Panasonic Electric Works is working among others on electro-mechanical products, such as contactors for battery disconnection or battery charging, on semi-conductor relays for battery monitoring and on complex systems as battery disconnect units. This paper will show experience on the hybrid vehicle sector. Further on different switching components and their usage will be introduced. As a main topic battery disconnected units will be discussed. Based on an actual example basic development items and system features will be touched and important development stages will be shown. As a general topic a future view on vehicles and batteries, as well as on charging systems and infrastructural necessities will be introduced. (orig.)

  11. Design of a Natural Gas Liquefaction System with Minimum Components

    International Nuclear Information System (INIS)

    Bergese, Franco

    2004-01-01

    In this work an economic method for liquefying natural gas by diminishing its temperature by means of the Joule-Thomson effect is presented.The pressures from and to which the gas must be expanded arose from a thermodynamic calculation optimizing the cost per unit mass of Liquefied Natural Gas LNG).It was determined that the gas should be expanded from 200 atm to 4 atm.This expansion ratio can be used in different scales.Large Scale: liquefaction of gas at well.It takes advantage of the fact that the gas inside the well is stored at high pressure.The gas is expanded in a valve / nozzle and then compressed to the pressure of the local pipeline system.The objective of this project is to export natural gas as LNG, which is transported by ships to the markets of consumption.Using this method of liquefaction, the LNG production levels are limited to a fraction of the production of the well, due to the injection of the un condensed gas into the local pipelines system.Medium Scale: A high pressure pipeline is the source of the gas.The expansion is performed and then the gas is again compressed to the pressure of a lower pressure pipeline into which the gas is injected.The pressure reductions of natural gas are performed nearby big cities.The aim of this project scale is the storage of fuel for gas thermal power plants during periods of low energy consumption for later burning when the resource is limited. Another possibility that offers this size of plant is the transportation of gas to regions where the resource is unavailable.This transportation would be carried out by means of cistern trucks, in the same way that conventional liquid fuels are transported.Small scale: the place of production would be a CNG refueling station. The source of gas is in this case a gas pipeline of urban distribution and the gas should be compressed with the compressor of the refueling station.Compressors have generally low loading factor and the periods of time when they are not producing

  12. The human component in the safety of complex systems

    International Nuclear Information System (INIS)

    Wahlstroem, B.

    1986-02-01

    The safety of nuclear power and other complex processes requires that human actions are carried though on time and without error. Investigations indicate that human errors are the main or an important contributing cause in more than half of the incidents which occur. This makes it important to try understand the mechanisms behind the human errors and to investigate possibilities for decreasing their likelihood. The present report presents an overview of the Nordic cooperation in the field of human factors in nuclear safety, under the LIT-programme carried out 1981-1985. The work was divided into six different projects in the following fields: human reliability in test and maintenance work; safety oriented organizations and company structures; design of information and control systems; new approaches for information presentation; experimental validation of man-machine interfaces; planning and evaluation of operator training. The research topics were selected from the findings of an earlier phase of the Nordic cooperation. The results are described in more detail in separate reports

  13. Reliability Analysis of 6-Component Star Markov Repairable System with Spatial Dependence

    Directory of Open Access Journals (Sweden)

    Liying Wang

    2017-01-01

    Full Text Available Star repairable systems with spatial dependence consist of a center component and several peripheral components. The peripheral components are arranged around the center component, and the performance of each component depends on its spatial “neighbors.” Vector-Markov process is adapted to describe the performance of the system. The state space and transition rate matrix corresponding to the 6-component star Markov repairable system with spatial dependence are presented via probability analysis method. Several reliability indices, such as the availability, the probabilities of visiting the safety, the degradation, the alert, and the failed state sets, are obtained by Laplace transform method and a numerical example is provided to illustrate the results.

  14. Anti-complement activities of human breast-milk.

    Science.gov (United States)

    Ogundele, M O

    1999-08-01

    It has long been observed that the human milk possesses significant anti-inflammatory properties, while simultaneously protecting the infant against many intestinal and respiratory pathogens. There is, however, a paucity of information on the degree and extent of this anti-inflammatory activity. In the present study, the inhibitory effects of different fractions of human milk on serum complement activity were analysed. Colostrum and milk samples from healthy voluntary lactating donors at different postpartum ages were obtained and pooled normal human serum was used as source of complement in a modified CH50 assay. Inherent complement activity in human milk was also investigated by measuring the deposition of an activated C3 fragment on a serum-sensitive bacteria, and by haemolytic assays. Most whole- and defatted-milk samples consistently showed a dose-dependent inhibition of the serum complement activity. This inhibition was greater in mature milk compared to transitional milk samples. It was enhanced by inactivation of milk complement, and diminished by centrifugation of milk samples, which partly removed fat and larger protein components including casein micelles. Inherent complement activity in human milk was also demonstrated by haemolysis of sensitised sheep erythrocytes and deposition of C3 fragments on solid-phase bacteria. These activities were highest in the colostrum and gradually decreased as lactation proceeded. Several natural components abundant in the fluid phase of the human breast-milk have been shown to be inhibitors of complement activation in vitro. Their physiological significance probably reside in their ability to prevent inflammatory-induced tissue damage of the delicate immature gastrointestinal tract of the new-born as well as the mammary gland itself, which may arise from ongoing complement activation.

  15. Reliability prediction of engineering systems with competing failure modes due to component degradation

    International Nuclear Information System (INIS)

    Son, Young Kap

    2011-01-01

    Reliability of an engineering system depends on two reliability metrics: the mechanical reliability, considering component failures, that a functional system topology is maintained and the performance reliability of adequate system performance in each functional configuration. Component degradation explains not only the component aging processes leading to failure in function, but also system performance change over time. Multiple competing failure modes for systems with degrading components in terms of system functionality and system performance are considered in this paper with the assumption that system functionality is not independent of system performance. To reduce errors in system reliability prediction, this paper tries to extend system performance reliability prediction methods in open literature through combining system mechanical reliability from component reliabilities and system performance reliability. The extended reliability prediction method provides a useful way to compare designs as well as to determine effective maintenance policy for efficient reliability growth. Application of the method to an electro-mechanical system, as an illustrative example, is explained in detail, and the prediction results are discussed. Both mechanical reliability and performance reliability are compared to total system reliability in terms of reliability prediction errors

  16. Analysis for a two-dissimilar-component cold standby repairable system with repair priority

    International Nuclear Information System (INIS)

    Leung, Kit Nam Francis; Zhang Yuanlin; Lai, Kin Keung

    2011-01-01

    In this paper, a cold standby repairable system consisting of two dissimilar components and one repairman is studied. Assume that working time distributions and repair time distributions of the two components are both exponential, and Component 1 has repair priority when both components are broken down. After repair, Component 1 follows a geometric process repair while Component 2 obeys a perfect repair. Under these assumptions, using the perfect repair model, the geometric process repair model and the supplementary variable technique, we not only study some important reliability indices, but also consider a replacement policy T, under which the system is replaced when the working age of Component 1 reaches T. Our problem is to determine an optimal policy T* such that the long-run average loss per unit time (i.e. average loss rate) of the system is minimized. The explicit expression for the average loss rate of the system is derived, and the corresponding optimal replacement policy T* can be found numerically. Finally, a numerical example for replacement policy T is given to illustrate some theoretical results and the model's applicability. - Highlights: → A two-dissimilar-component cold standby system with repair priority is formulated. → The successive up/repair times of Component 1 form a decreasing/increasing geometric process. → Not only some reliability indices but also a replacement policy are studied.

  17. Reliability Analysis of Load-Sharing K-out-of-N System Considering Component Degradation

    Directory of Open Access Journals (Sweden)

    Chunbo Yang

    2015-01-01

    Full Text Available The K-out-of-N configuration is a typical form of redundancy techniques to improve system reliability, where at least K-out-of-N components must work for successful operation of system. When the components are degraded, more components are needed to meet the system requirement, which means that the value of K has to increase. The current reliability analysis methods overestimate the reliability, because using constant K ignores the degradation effect. In a load-sharing system with degrading components, the workload shared on each surviving component will increase after a random component failure, resulting in higher failure rate and increased performance degradation rate. This paper proposes a method combining a tampered failure rate model with a performance degradation model to analyze the reliability of load-sharing K-out-of-N system with degrading components. The proposed method considers the value of K as a variable which is derived by the performance degradation model. Also, the load-sharing effect is evaluated by the tampered failure rate model. Monte-Carlo simulation procedure is used to estimate the discrete probability distribution of K. The case of a solar panel is studied in this paper, and the result shows that the reliability considering component degradation is less than that ignoring component degradation.

  18. Plasma complement and vascular complement deposition in patients with coronary artery disease with and without inflammatory rheumatic diseases

    Science.gov (United States)

    2017-01-01

    Purpose Inflammatory rheumatic diseases (IRD) are associated with accelerated coronary artery disease (CAD), which may result from both systemic and vascular wall inflammation. There are indications that complement may be involved in the pathogenesis of CAD in Systemic Lupus Erythematosus (SLE) and Rheumatoid Arthritis (RA). This study aimed to evaluate the associations between circulating complement and complement activation products with mononuclear cell infiltrates (MCI, surrogate marker of vascular inflammation) in the aortic media and adventitia in IRDCAD and non-IRDCAD patients undergoing coronary artery bypass grafting (CABG). Furthermore, we compared complement activation product deposition patterns in rare aorta adventitial and medial biopsies from SLE, RA and non-IRD patients. Methods We examined plasma C3 (p-C3) and terminal complement complexes (p-TCC) in 28 IRDCAD (SLE = 3; RA = 25), 52 non-IRDCAD patients, and 32 IRDNo CAD (RA = 32) from the Feiring Heart Biopsy Study. Aortic biopsies taken from the CAD only patients during CABG were previously evaluated for adventitial MCIs. The rare aortic biopsies from 3 SLE, 3 RA and 3 non-IRDCAD were assessed for the presence of C3 and C3d using immunohistochemistry. Results IRDCAD patients had higher p-TCC than non-IRDCAD or IRDNo CAD patients (prheumatic disease, and, in particular, SLE with the complement system. Exaggerated systemic and vascular complement activation may accelerate CVD, serve as a CVD biomarker, and represent a target for new therapies. PMID:28362874

  19. Methods of producing epoxides from alkenes using a two-component catalyst system

    Science.gov (United States)

    Kung, Mayfair C.; Kung, Harold H.; Jiang, Jian

    2013-07-09

    Methods for the epoxidation of alkenes are provided. The methods include the steps of exposing the alkene to a two-component catalyst system in an aqueous solution in the presence of carbon monoxide and molecular oxygen under conditions in which the alkene is epoxidized. The two-component catalyst system comprises a first catalyst that generates peroxides or peroxy intermediates during oxidation of CO with molecular oxygen and a second catalyst that catalyzes the epoxidation of the alkene using the peroxides or peroxy intermediates. A catalyst system composed of particles of suspended gold and titanium silicalite is one example of a suitable two-component catalyst system.

  20. The MainSTREAM Component Platform: A Holistic Approach to Microfluidic System Design

    DEFF Research Database (Denmark)

    Sabourin, David; Skafte-Pedersen, Peder; Søe, Martin Jensen

    2013-01-01

    A microfluidic component library for building systems driving parallel or serial microfluidic-based assays is presented. The components are a miniaturized eight-channel peristaltic pump, an eight-channel valve, sample-to-waste liquid management, and interconnections. The library of components...... of reaction chips; (2) highly parallel pumping and routing/valving capability; (3) methods to interface pumps and chip-to-liquid management systems; (4) means to construct a portable system; (5) reconfigurability/flexibility in system design; (6) means to interface to microscopes; and (7) compatibility...

  1. Methods for removal of unwanted signals from gravity time-series: Comparison using linear techniques complemented with analysis of system dynamics

    Science.gov (United States)

    Valencio, Arthur; Grebogi, Celso; Baptista, Murilo S.

    2017-10-01

    The presence of undesirable dominating signals in geophysical experimental data is a challenge in many subfields. One remarkable example is surface gravimetry, where frequencies from Earth tides correspond to time-series fluctuations up to a thousand times larger than the phenomena of major interest, such as hydrological gravity effects or co-seismic gravity changes. This work discusses general methods for the removal of unwanted dominating signals by applying them to 8 long-period gravity time-series of the International Geodynamics and Earth Tides Service, equivalent to the acquisition from 8 instruments in 5 locations representative of the network. We compare three different conceptual approaches for tide removal: frequency filtering, physical modelling, and data-based modelling. Each approach reveals a different limitation to be considered depending on the intended application. Vestiges of tides remain in the residues for the modelling procedures, whereas the signal was distorted in different ways by the filtering and data-based procedures. The linear techniques employed were power spectral density, spectrogram, cross-correlation, and classical harmonics decomposition, while the system dynamics was analysed by state-space reconstruction and estimation of the largest Lyapunov exponent. Although the tides could not be completely eliminated, they were sufficiently reduced to allow observation of geophysical events of interest above the 10 nm s-2 level, exemplified by a hydrology-related event of 60 nm s-2. The implementations adopted for each conceptual approach are general, so that their principles could be applied to other kinds of data affected by undesired signals composed mainly by periodic or quasi-periodic components.

  2. Analysis on Sealing Reliability of Bolted Joint Ball Head Component of Satellite Propulsion System

    Science.gov (United States)

    Guo, Tao; Fan, Yougao; Gao, Feng; Gu, Shixin; Wang, Wei

    2018-01-01

    Propulsion system is one of the important subsystems of satellite, and its performance directly affects the service life, attitude control and reliability of the satellite. The Paper analyzes the sealing principle of bolted joint ball head component of satellite propulsion system and discuss from the compatibility of hydrazine anhydrous and bolted joint ball head component, influence of ground environment on the sealing performance of bolted joint ball heads, and material failure caused by environment, showing that the sealing reliability of bolted joint ball head component is good and the influence of above three aspects on sealing of bolted joint ball head component can be ignored.

  3. Advanced Surveillance, Diagnostic and Prognostic Techniques in Monitoring Structures, Systems and Components in Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2013-09-15

    direction of research, development and demonstration in this area. The technologies discussed in this project are intended to establish the state of the art in surveillance, diagnostics and prognostics (SDP) technologies for equipment and process health monitoring in nuclear facilities. It is also intended to identify technology gaps and research needs of the nuclear industry in the area of SDP. The report draws on the conventional SDP technologies, as well as the latest tools, algorithms and techniques that have emerged over the last few years, especially in enabling technologies including fast data acquisition, data storage, data qualification and data analysis algorithms, such as empirical and physical modelling techniques. These new tools have made it possible to identify problems earlier and with better resolution. The significance of the material presented in this report is that it contributes not only to the current needs of the nuclear industry but also to the design improvements of the next generation of reactors. For example, the nuclear industry is currently striving to operate the plants for up to 80 years or more, as the value of nuclear assets has risen in recent years, resulting partly from environmental concerns with fossil energy production, as well as increased future demand for base load electricity. This long term operation (LTO) or life extension goal of the nuclear industry has stimulated renewed interest in more frequent monitoring of equipment to guard against ageing effects, not to mention the economic benefits that SDP implementation can produce, and contributions to radiation exposure that is as low as reasonably achievable, reduction of human errors, and optimized maintenance. Together with capabilities that enhance situational awareness, the technologies described in this report will enable more holistic management of plant structures, systems and components (SSCs), maintain high capacity factor in LTO and enable higher levels of safe operation

  4. Advanced Surveillance, Diagnostic and Prognostic Techniques in Monitoring Structures, Systems and Components in Nuclear Power Plants

    International Nuclear Information System (INIS)

    2013-01-01

    direction of research, development and demonstration in this area. The technologies discussed in this project are intended to establish the state of the art in surveillance, diagnostics and prognostics (SDP) technologies for equipment and process health monitoring in nuclear facilities. It is also intended to identify technology gaps and research needs of the nuclear industry in the area of SDP. The report draws on the conventional SDP technologies, as well as the latest tools, algorithms and techniques that have emerged over the last few years, especially in enabling technologies including fast data acquisition, data storage, data qualification and data analysis algorithms, such as empirical and physical modelling techniques. These new tools have made it possible to identify problems earlier and with better resolution. The significance of the material presented in this report is that it contributes not only to the current needs of the nuclear industry but also to the design improvements of the next generation of reactors. For example, the nuclear industry is currently striving to operate the plants for up to 80 years or more, as the value of nuclear assets has risen in recent years, resulting partly from environmental concerns with fossil energy production, as well as increased future demand for base load electricity. This long term operation (LTO) or life extension goal of the nuclear industry has stimulated renewed interest in more frequent monitoring of equipment to guard against ageing effects, not to mention the economic benefits that SDP implementation can produce, and contributions to radiation exposure that is as low as reasonably achievable, reduction of human errors, and optimized maintenance. Together with capabilities that enhance situational awareness, the technologies described in this report will enable more holistic management of plant structures, systems and components (SSCs), maintain high capacity factor in LTO and enable higher levels of safe operation

  5. Chloroplast two-component systems: evolution of the link between photosynthesis and gene expression.

    Science.gov (United States)

    Puthiyaveetil, Sujith; Allen, John F

    2009-06-22

    Two-component signal transduction, consisting of sensor kinases and response regulators, is the predominant signalling mechanism in bacteria. This signalling system originated in prokaryotes and has spread throughout the eukaryotic domain of life through endosymbiotic, lateral gene transfer from the bacterial ancestors and early evolutionary precursors of eukaryotic, cytoplasmic, bioenergetic organelles-chloroplasts and mitochondria. Until recently, it was thought that two-component systems inherited from an ancestral cyanobacterial symbiont are no longer present in chloroplasts. Recent research now shows that two-component systems have survived in chloroplasts as products of both chloroplast and nuclear genes. Comparative genomic analysis of photosynthetic eukaryotes shows a lineage-specific distribution of chloroplast two-component systems. The components and the systems they comprise have homologues in extant cyanobacterial lineages, indicating their ancient cyanobacterial origin. Sequence and functional characteristics of chloroplast two-component systems point to their fundamental role in linking photosynthesis with gene expression. We propose that two-component systems provide a coupling between photosynthesis and gene expression that serves to retain genes in chloroplasts, thus providing the basis of cytoplasmic, non-Mendelian inheritance of plastid-associated characters. We discuss the role of this coupling in the chronobiology of cells and in the dialogue between nuclear and cytoplasmic genetic systems.

  6. Complement's participation in acquired immunity

    DEFF Research Database (Denmark)

    Nielsen, Claus Henrik; Leslie, Robert Graham Quinton

    2002-01-01

    of the B cell receptor for antigen (BCR), a complex composed of the iC3b/C3d fragment-binding complement type 2 receptor (CR2, CD21) and its signaling element CD19 and the IgG-binding receptor FcgammaRIIb (CD32). The positive or negative outcome of signaling through this triad is determined by the context...

  7. Sensitivity analysis on the component cooling system of the Angra 1 NPP

    International Nuclear Information System (INIS)

    Castro Silva, Luiz Euripedes Massiere de

    1995-01-01

    The component cooling system has been studied within the scope of the Probabilistic Safety Analysis of the Angra I NPP in order to assure that the proposed modelling suits as close as possible the functioning system and its availability aspects. In such a way a sensitivity analysis was performed on the equivalence between the operating modes of the component cooling system and its results show the fitness of the model. (author). 4 refs, 3 figs, 3 tabs

  8. Estimation of component failure probability from masked binomial system testing data

    International Nuclear Information System (INIS)

    Tan Zhibin

    2005-01-01

    The component failure probability estimates from analysis of binomial system testing data are very useful because they reflect the operational failure probability of components in the field which is similar to the test environment. In practice, this type of analysis is often confounded by the problem of data masking: the status of tested components is unknown. Methods in considering this type of uncertainty are usually computationally intensive and not practical to solve the problem for complex systems. In this paper, we consider masked binomial system testing data and develop a probabilistic model to efficiently estimate component failure probabilities. In the model, all system tests are classified into test categories based on component coverage. Component coverage of test categories is modeled by a bipartite graph. Test category failure probabilities conditional on the status of covered components are defined. An EM algorithm to estimate component failure probabilities is developed based on a simple but powerful concept: equivalent failures and tests. By simulation we not only demonstrate the convergence and accuracy of the algorithm but also show that the probabilistic model is capable of analyzing systems in series, parallel and any other user defined structures. A case study illustrates an application in test case prioritization

  9. Reliability analysis of nuclear component cooling water system using semi-Markov process model

    International Nuclear Information System (INIS)

    Veeramany, Arun; Pandey, Mahesh D.

    2011-01-01

    Research highlights: → Semi-Markov process (SMP) model is used to evaluate system failure probability of the nuclear component cooling water (NCCW) system. → SMP is used because it can solve reliability block diagram with a mixture of redundant repairable and non-repairable components. → The primary objective is to demonstrate that SMP can consider Weibull failure time distribution for components while a Markov model cannot → Result: the variability in component failure time is directly proportional to the NCCW system failure probability. → The result can be utilized as an initiating event probability in probabilistic safety assessment projects. - Abstract: A reliability analysis of nuclear component cooling water (NCCW) system is carried out. Semi-Markov process model is used in the analysis because it has potential to solve a reliability block diagram with a mixture of repairable and non-repairable components. With Markov models it is only possible to assume an exponential profile for component failure times. An advantage of the proposed model is the ability to assume Weibull distribution for the failure time of components. In an attempt to reduce the number of states in the model, it is shown that usage of poly-Weibull distribution arises. The objective of the paper is to determine system failure probability under these assumptions. Monte Carlo simulation is used to validate the model result. This result can be utilized as an initiating event probability in probabilistic safety assessment projects.

  10. Design and Application of an Ontology for Component-Based Modeling of Water Systems

    Science.gov (United States)

    Elag, M.; Goodall, J. L.

    2012-12-01

    Many Earth system modeling frameworks have adopted an approach of componentizing models so that a large model can be assembled by linking a set of smaller model components. These model components can then be more easily reused, extended, and maintained by a large group of model developers and end users. While there has been a notable increase in component-based model frameworks in the Earth sciences in recent years, there has been less work on creating framework-agnostic metadata and ontologies for model components. Well defined model component metadata is needed, however, to facilitate sharing, reuse, and interoperability both within and across Earth system modeling frameworks. To address this need, we have designed an ontology for the water resources community named the Water Resources Component (WRC) ontology in order to advance the application of component-based modeling frameworks across water related disciplines. Here we present the design of the WRC ontology and demonstrate its application for integration of model components used in watershed management. First we show how the watershed modeling system Soil and Water Assessment Tool (SWAT) can be decomposed into a set of hydrological and ecological components that adopt the Open Modeling Interface (OpenMI) standard. Then we show how the components can be used to estimate nitrogen losses from land to surface water for the Baltimore Ecosystem study area. Results of this work are (i) a demonstration of how the WRC ontology advances the conceptual integration between components of water related disciplines by handling the semantic and syntactic heterogeneity present when describing components from different disciplines and (ii) an investigation of a methodology by which large models can be decomposed into a set of model components that can be well described by populating metadata according to the WRC ontology.

  11. Fluoride in the Serra Geral Aquifer System: Source Evaluation Using Stable Isotopes and Principal Component Analysis

    OpenAIRE

    Nanni, Arthur Schmidt; Roisenberg, Ari; de Hollanda, Maria Helena Bezerra Maia; Marimon, Maria Paula Casagrande; Viero, Antonio Pedro; Scheibe, Luiz Fernando

    2013-01-01

    Groundwater with anomalous fluoride content and water mixture patterns were studied in the fractured Serra Geral Aquifer System, a basaltic to rhyolitic geological unit, using a principal component analysis interpretation of groundwater chemical data from 309 deep wells distributed in the Rio Grande do Sul State, Southern Brazil. A four-component model that explains 81% of the total variance in the Principal Component Analysis is suggested. Six hydrochemical groups were identified. δ18O and δ...

  12. A Component-Based Modeling and Validation Method for PLC Systems

    Directory of Open Access Journals (Sweden)

    Rui Wang

    2014-05-01

    Full Text Available Programmable logic controllers (PLCs are complex embedded systems that are widely used in industry. This paper presents a component-based modeling and validation method for PLC systems using the behavior-interaction-priority (BIP framework. We designed a general system architecture and a component library for a type of device control system. The control software and hardware of the environment were all modeled as BIP components. System requirements were formalized as monitors. Simulation was carried out to validate the system model. A realistic example from industry of the gates control system was employed to illustrate our strategies. We found a couple of design errors during the simulation, which helped us to improve the dependability of the original systems. The results of experiment demonstrated the effectiveness of our approach.

  13. Safety philosophy and design principles for systems and components of nuclear power plant: external event

    International Nuclear Information System (INIS)

    Lopes, J.P.G.

    1986-01-01

    In nuclear power plants, some systems and components are designed to withstand external impacts. Such systems and components are those which have to perform their functions even during and after the occurrences of an earthquake, for example, fulfilling the safety objectives and avoiding the release of radioactive material to the environment. The aim of this report is to introduce the safety philosophy and design principles for systems/components to perform their functions during and after the occurrence of an earthquake, as applied by NUCLEN for Angra 2 and 3. (Author) [pt

  14. Analysis of appraisal tool of system security engineering capability maturity based on component

    International Nuclear Information System (INIS)

    Liu Zhenghai; Yang Xiaohua; Zou Shuliang; Liu Yachun; Xiao Jiantian; Liu Zhiming

    2012-01-01

    Spent Fuel Reprocessing is a part of nuclear fuel cycle and is the inevitably choice of nuclear power sustainable development. Reprocessing needs to face with radiological, criticality, chemical hazards. Besides using the tradition appraisal methods based on the security goals, it is a beneficial supplement that using the appraisal method of system security engineering capability maturity model based on the process. Experts should check and approve large numbers of documents during the appraisal based on system security engineering capability maturity model, so it is necessary that developing a tool to assist the expert to complete the appraisal. The method of developing software based on component is highly effective, nimble and reliable. Component technology is analyzed, the methods of extraction model domain components and general components is introduced, and the appraisal system is developed based on component technology. (authors)

  15. Electrostatic Discharge (ESD and Electrical Overstress (EOS: The state of the art in components to systems

    Directory of Open Access Journals (Sweden)

    Steven H. Voldman

    2017-06-01

    Full Text Available Electrostatic Discharge (ESD, Electrical Overstress (EOS and electromagnetic compatibility (EMC continue to impact semiconductor manufacturing, semiconductor components and systems as technologies scale from micro- to nano-electronics. The range of concern for components include semiconductor components, magnetic recording industry, MEMs, and for products from disk drives, cell phones, notebooks, tablets, laptops, and desktop computers. The objective of this lecture is to address the state of the art of electrostatic discharge (ESD and electrical overstress (EOS in today’s electronic components and systems. The tutorial provides a clear picture of ESD, EOS and EMC phenomena, sources, physics, failure mechanisms, testing and qualification of components and systems. The conclusion of this talk is that ESD and EOS continue to be a concern in technologies from micro-electronics to nano-structures, and will remain a reliability and quality issue in the future.

  16. Classification of transportation packaging and dry spent fuel storage system components according to importance to safety

    International Nuclear Information System (INIS)

    Tyacke, M.J.; McConnell, J.W. Jr.; Ayers, A.L. Jr.; O'Connor, S.C.; Jankovich, J.P.

    1996-01-01

    The Idaho National Engineering Laboratory prepared a technical report for the Office of Nuclear Material Safety and Safeguards of the US Nuclear Regulatory Commission, entitled Classification of Transportation Packaging and Dry Spent Fuel Storage System Components According to Importance to Safety, NUREG/CR-6407. This paper provides the results of that report. It also presents the graded approach for classification of components used in transportation packagings and dry spent fuel storage systems. This approach provides a method for identifying the classification of components according to importance to safety within transportation packagings and dry spent fuel storage systems. Record retention requirements are discussed to identify the documentation necessary to validate that the individual components were fabricated in accordance with their assigned classification. A review of the existing regulations pertaining to transportation packagings and dry storage systems was performed to identify current requirements. The general types of transportation packagings and dry storage systems are identified. The methodology used in this paper is based on Regulatory Guide 7.10, Establishing Quality Assurance Programs for Packaging Used in the Transport of Radioactive Material. This paper also includes a list of generic components for each of the general types of transportation packagings and spent fuel storage systems, with a classification category assigned to each component. Several examples concerning the safety importance of components are presented

  17. Development of prototype components for the silicon tracking system of the CBM experiment at FAIR

    Energy Technology Data Exchange (ETDEWEB)

    Lymanets, Anton

    2013-06-26

    The CBM experiment at future accelerator facility FAIR will investigate the properties of nuclear matter under extreme conditions. The experimental programm is different from the heavy-ion experiments at RHIC (BNL) and LHC (CERN) that create nuclear matter at high temperatures. In contrast, the study of the QCD phase diagram in the region of the highest net baryon densities and moderate temperatures that is weakly explored will be performed with high precision. For this, collisions of different heavy-ion beams at the energies of 10-45 GeV/nucleon with nuclear target will be measured. The physics programme of the CBM experiment includes measurement of both rare probes and bulk observables that originate from various phases of a nucleus-nucleus collision. In particular, decay of particles with charm quarks can be registered by reconstructing the decay vertex detached from the primary interaction point by several hundreds of micrometers (e.g., decay length cτ=123 μm for D{sup 0} meson). For this, precise tracking and full event reconstruction with up to 600 charged particle tracks per event within acceptance are required. Other rare probes require operation at interaction rate of up to 10 MHz. The detector system that performs tracking has to provide high position resolution on the order of 10 μm, operate at high rates and have radiation tolerant design with low material budget. The Silicon Tracking System (STS) is being designed for charged-particle tracking in a magnetic field. The system consists of eight tracking station located in the aperture of a dipole magnet with 1 T field. For tracks with momentum above 1 GeV, momentum resolution of such a system is expected to be about 1%. In order to fulfill this task, thorough optimization of the detector design is required. In particular, minimal material budget has to be achieved. Production of a detector module requires research and development activities with respect to the module components and their integration

  18. Study on system layout and component design in the HTTR hydrogen production system. Contract research

    International Nuclear Information System (INIS)

    Nishihara, Tetsuo; Shimizu, Akira; Uchida, Shoji

    2003-01-01

    The global warming becomes a significant issue in the world so that it needs to reduce the CO 2 emission. It is expected that hydrogen is in place of the fossil fuels such as coal and oil, and plays the important role to resolve the global warming. There are several hydrogen making processes such as water electrolysis and steam reforming of hydrocarbon. Steam reforming of hydrocarbon is a major hydrogen making process because of economy in industry. It utilizes the fossil fuels as process heat for chemical reaction and results in a large CO 2 emission. New steam reforming system without fossil fuel can contribute to resolve the global warming. High temperature gas-cooled reactor (HTGR) has a unique feature to be able to supply a hot helium gas whose temperature is approximately 950degC at the reactor outlet. This makes HTGR possible to utilize for not only power generation but also process heat utilization. JAERI constructed the high temperature engineering test reactor (HTTR) that is a sort of HTGR in Oarai establishment and starts operation. Nuclear heat utilization is one of the R and D items of the HTTR. The steam reforming system coupling to the HTTR for hydrogen production has been designed. This report represents the system layout and design specification of key components in HTTR steam reforming system. (author)

  19. Study on system layout and component design in the HTTR hydrogen production system. Contract research

    Energy Technology Data Exchange (ETDEWEB)

    Nishihara, Tetsuo; Shimizu, Akira [Japan Atomic Energy Research Inst., Oarai, Ibaraki (Japan). Oarai Research Establishment; Tanihira, Masanori [Mitsubishi Heavy Industries Ltd., Tokyo (Japan); Uchida, Shoji [Advanced Reactor Technology Co., Ltd., Tokyo (Japan)

    2003-01-01

    The global warming becomes a significant issue in the world so that it needs to reduce the CO{sub 2} emission. It is expected that hydrogen is in place of the fossil fuels such as coal and oil, and plays the important role to resolve the global warming. There are several hydrogen making processes such as water electrolysis and steam reforming of hydrocarbon. Steam reforming of hydrocarbon is a major hydrogen making process because of economy in industry. It utilizes the fossil fuels as process heat for chemical reaction and results in a large CO{sub 2} emission. New steam reforming system without fossil fuel can contribute to resolve the global warming. High temperature gas-cooled reactor (HTGR) has a unique feature to be able to supply a hot helium gas whose temperature is approximately 950degC at the reactor outlet. This makes HTGR possible to utilize for not only power generation but also process heat utilization. JAERI constructed the high temperature engineering test reactor (HTTR) that is a sort of HTGR in Oarai establishment and starts operation. Nuclear heat utilization is one of the R and D items of the HTTR. The steam reforming system coupling to the HTTR for hydrogen production has been designed. This report represents the system layout and design specification of key components in HTTR steam reforming system. (author)

  20. A Study on Components of Internal Control-Based Administrative System in Secondary Schools

    Science.gov (United States)

    Montri, Paitoon; Sirisuth, Chaiyuth; Lammana, Preeda

    2015-01-01

    The aim of this study was to study the components of the internal control-based administrative system in secondary schools, and make a Confirmatory Factor Analysis (CFA) to confirm the goodness of fit of empirical data and component model that resulted from the CFA. The study consisted of three steps: 1) studying of principles, ideas, and theories…