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Sample records for citrus limonia osbeck

  1. In silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck under water stress

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    Raquel L. Boscariol-Camargo

    2007-01-01

    Full Text Available CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and proline-related synthase. Some sequences could not be classified by using FunCat and remained with an unknown function. A large number of sequences presented high similarities to annotated genes involved with cell energy, protein synthesis and cellular transport, suggesting that Rangpur lime may sustain active cell growth under stressed condition. The presence of membrane transporters and cell signaling components could be an indication of a coordinated morphological adaptation and biochemical response during drought, helping to explain the higher tolerance of this rootstock to water stress.

  2. EFEITOS DE GIBERELINAS, CITOCININAS E DO NITRATO DE POTÁSSIO, NO PROCESSO GERMINATIVO DE SEMENTES DE LIMOEIRO `CRAVO' (Citrus limonia OSBECK

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    Leonel Sarita

    1999-01-01

    Full Text Available Foram estudados os efeitos de reguladores vegetais do grupo das giberelinas e citocininas, bem como do nitrato de potássio na germinação de sementes do limoeiro `Cravo' (Citrus limonia Osbeck. O experimento foi realizado, contendo papel de filtro como substrato para a germinação das sementes, regulado à temperatura de 25oC. As sementes foram retiradas de frutos maduros no final da safra do limoeiro `Cravo', lavadas, secas à sombra e armazenadas durante 11 dias em câmara fria. Em seguida, receberam tratamento com os fitorreguladores e KNO3 por 24 horas, de acordo com os tratamentos: KNO3 0,1% e 0,2%; GA3 50, 100 e 250 mg.L-1; GA4 + GA7 + fenilmetilaminopurina 100 mg.L-1; fenilmetilaminopurina 20 mg.L-1 e água destilada (testemunha. As avaliações foram iniciadas 15 dias após a semeadura, em intervalos de 5 dias. Conclui-se que os reguladores vegetais utilizados não afetaram o processo germinativo das sementes e que os tratamentos com nitrato de potássio 0,1% e 0,2% exerceram efeito inibitório sobre a germinação.

  3. Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments

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    Almeida Weliton Antonio Bastos de

    2003-01-01

    Full Text Available Genetic transformation allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. The objective of this research was to establish a protocol for genetic transformation of Valencia and Natal sweet oranges (Citrus sinensis L. Osbeck and Rangpur lime (Citrus limonia L. Osbeck. Epicotyl segments of germinated in vitro plantlets (three weeks in darkness and two weeks in a 16-h photoperiod were used as explants. These were co-cultivated with Agrobacterium tumefaciens strain EHA-105 and different experiments were done to evaluate the transformation efficiency: explants were co-cultivated with Agrobacterium for one, three or five days; explants were incubated with Agrobacterium suspension for 5, 10, 20 or 40 minutes; co-cultivation medium was supplemented with acetosyringone at 0, 100 or 200 mmol L-1; Explants ends had a longitudinal terminal incision (2-3 mm; co-cultivation temperatures of 19, 23 or 27degreesC were imposed. The experimental design was completely randomized in all experiments with five replications, each consisted of a Petri dish (100 x 15 mm with 30 explants and resulted in a total of 150 explants per treatment. Longitudinal terminal incision in the explant ends did not improve shoot regeneration. However, transgenic plants of all three cultivars were confirmed from explants that had been subjected to inoculation time of 20 minutes, co-culture of three days at 23-27degreesC, in the absence of acetosyringone.

  4. Large changes in anatomy and physiology between diploid Rangpur lime (Citrus limonia) and its autotetraploid are not associated with large changes in leaf gene expression.

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    Allario, Thierry; Brumos, Javier; Colmenero-Flores, Jose Manuel; Tadeo, Francisco; Froelicher, Yann; Talon, Manuel; Navarro, Luis; Ollitrault, Patrick; Morillon, Raphaël

    2011-05-01

    Very little is known about the molecular origin of the large phenotypic differentiation between genotypes arising from somatic chromosome set doubling and their diploid parents. In this study, the anatomy and physiology of diploid (2x) and autotetraploid (4x) Rangpur lime (Citrus limonia Osbeck) seedlings has been characterized. Growth of 2x was more vigorous than 4x although leaves, stems, and roots of 4x plants were thicker and contained larger cells than 2x that may have a large impact on cell-to-cell water exchanges. Leaf water content was higher in 4x than in 2x. Leaf transcriptome expression using a citrus microarray containing 21 081 genes revealed that the number of genes differentially expressed in both genotypes was less than 1% and the maximum rate of gene expression change within a 2-fold range. Six up-regulated genes in 4x were targeted to validate microarray results by real-time reverse transcription-PCR. Five of these genes were apparently involved in the response to water deficit, suggesting that, in control conditions, the genome expression of citrus autotetraploids may act in a similar way to diploids under water-deficit stress condition. The sixth up-regulated gene which codes for a histone may also play an important role in regulating the transcription of growth processes. These results show that the large phenotypic differentiation in 4x Rangpur lime compared with 2x is not associated with large changes in genome expression. This suggests that, in 4x Rangpur lime, subtle changes in gene expression may be at the origin of the phenotypic differentiation of 4x citrus when compared with 2x.

  5. Accumulation of the sesquiterpenes nootkatone and valencene by callus cultures of Citrus paradisi, Citrus limonia and Citrus aurantium.

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    Del Río, J A; Ortuño, A; Puig, D G; Iborra, J L; Sabater, F

    1991-10-01

    The production of the sesquiterpenes nootkatone and valencene by callus cultures of Citrus species is described. The levels of these compounds were examined by gas chromatography-mass spectrometry and their yields were compared with the amounts found in mature fruits. A simultaneous increase and decrease in the levels of nootkatone and valencene, respectively, were observed with the aging of callus cultures of Citrus paradisi. These results suggest that valencene might be a possible precursor of nootkatone in this species. The high level of nootkatone detected in 9-month-old callus cultures of Citrus paradisi might be associated with the corresponding cell morphological changes observed. PMID:24221735

  6. Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.

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    Soares, Márcio Santos; da Silva, Danielle Fernandes; Forim, Moacir Rossi; da Silva, Maria Fátima das Graças Fernandes; Fernandes, João Batista; Vieira, Paulo Cezar; Silva, Denise Brentan; Lopes, Norberto Peporine; de Carvalho, Sérgio Alves; de Souza, Alessandra Alves; Machado, Marcos Antônio

    2015-07-01

    A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for quantifying hesperidin and rutin levels in leaves and stems of Citrus limonia, with a good linearity over a range of 1.0-80.0 and 1.0-50.0 μg mL(-1) respectively, with r(2)>0.999 for all curves. The limits of detection (LOD) for both flavonoids were 0.6 and 0.5 μg mL(-1), respectively, with quantification (LOQ) being 2.0 and 1.0 μg mL(-1), respectively. The quantification method was applied to Citrus sinensis grafted onto C. limonia with and without CVC (citrus variegated chlorosis) symptoms after Xylella fastidiosa infection. The total content of rutin was low and practically constant in all analyses in comparison with hesperidin, which showed a significant increase in its amount in symptomatic leaves. Scanning electron microscopy studies on leaves with CVC symptoms showed vessel occlusion by biofilm, and a crystallized material was noted. Considering the difficulty in isolating these crystals for analysis, tissue sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) to confirm the presence of hesperidin at the site of infection. The images constructed from MS/MS data with a specific diagnostic fragment ion (m/z 483) also showed higher ion intensities for it in infected plants than in healthy ones, mainly in the vessel regions. These data suggest that hesperidin plays a role in the plant-pathogen interaction, probably as a phytoanticipin. This method was also applied to C. sinensis and C. limonia seedlings, and comparison with the graft results showed that the rootstock had an increased hesperidin content ∼3.6 fold greater in the graft stem than in the stem of C. sinensis seedlings. Increase in hesperidin content by rootstock can be related to induced internal defense mechanisms. PMID:25749617

  7. Performance of 'Valencia' Orange (Citrus sinensis [L.] Osbeck) on 17 rootstocks in a trial severely affected by huanglongbing

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    Valencia orange (Citrus sinensis L. Osbeck) was grown on 17 rootstocks through seven years of age and the first four harvest seasons in a central Florida field trial severely affected by huanglongbing (HLB) disease. All trees in the trial had huanglongbing symptoms and were shown by Polymerase chain...

  8. Isolation and Characterization of Copia-like Retrotransposons from 12 Sweet Orange (Citrus sinensis Osbeck) Cultivars

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    Neng-Guo TAO; Juan XU; Yun-Jiang CHENG; Liu HONG; Wen-Wu GUO; Hua-Lin YI; Xiu-Xin DENG

    2005-01-01

    As the largest transposable element in the plant genome, retrotransposons are thought to be involved in citrus genetic instability and genome evolution, especially in sweet orange, which is prone to bud mutation. In the present study, the presence of copia-like retrotransposons, their heterogeneity, genomic distribution, and transcriptional activities in Citrus were investigated in 12 sweet orange (Citrus sinensis Osbeck) cultivars using a PCR assay designed to detect copia-like reverse transcriptase (RT)sequences. Twelve amplification products from each cultivar were cloned and sequenced. The cloned sequences showed great heterogeneity, except "Dream" navel and "Hamlin", both of which shared the same sequence. Frame shifting, termination, deletion, and substitution accounted for the heterogeneity of RT sequences. Southern blot hybridization using the RT1 clone from the "Cara Cara" navel as a probe showed that multiple copies were integrated throughout the sweet orange genomes, which made the retrotransposon possible an effective molecular marker to detect citrus evolution events and to reveal its relationship with bud mutation. No transcriptional activities of the retrotransposon were detected by RT-PCR and Northern analysis in the fruits and leaves of either "Cara Cara" or "Seike" navels.

  9. Clinical evaluation of Moro (Citrus sinensis (L.) Osbeck) orange juice supplementation for the weight management.

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    Cardile, Venera; Graziano, Adriana Carol Eleonora; Venditti, Alessandro

    2015-01-01

    In the last years, several studies have recently evaluated the beneficial effects of red orange juice (Citrus sinensis (L.) Osbeck) and its active components in weight management and obesity. Moro orange is a cultivar of red orange, particularly rich in active compounds such as anthocyanins, hydroxycinnamic acids, flavone glycosides and ascorbic acid, which displays anti-obesity effects in in vitro and in vivo studies. In this clinical study, the effect of a Moro juice extract (Morosil(®), 400 mg/die) supplementation was evaluated in overweight healthy human volunteers for 12 weeks. Results showed that Moro juice extract intake was able to induce a significant reduction in body mass index (BMI) after 4 weeks of treatment (p juice have a synergistic effect on fat accumulation in humans and Moro juice extract can be used in weight management and in the prevention of human obesity.

  10. Supercritical Carbon Dioxide Extraction of Flavonoids from Pomelo (Citrus grandis (L.) Osbeck) Peel and Their Antioxidant Activity

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    Qiao-Mei Ru; Jian-Hua Liu; Ping Shao; Jin-Zhe He

    2012-01-01

    Supercritical carbon dioxide (SC-CO2) extraction of flavonoids from pomelo (Citrus grandis (L.) Osbeck) peel and their antioxidant activity were investigated. Box-Behnken design combined with response surface methodology was employed to maximize the extraction yield of flavonoids. Correlation analysis of the mathematical-regression model indicated that a quadratic polynomial model could be used to optimize the SC-CO2 extraction of flavonoids. The optimal conditions for obtaining the highest e...

  11. Analysis of expressed sequence tags from Citrus sinensis L. Osbeck infected with Xylella fastidiosa

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    Alessandra A. de Souza

    2007-01-01

    Full Text Available In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L. Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were associated with metabolism, protein modification, energy and transport facilitation. The majority of the up-regulated transcripts were associated with metabolism and defense response. Some transcripts associated with adaptation to stress conditions were up-regulated in plants with CVC and could explain why plants remain alive even under severe water and nutritional stress. Others of the up-regulated transcripts are related to defense response suggesting that sweet orange plants activate their defense machinery. The genes associated with stress response might be expressed as part of a secondary response related to physiological alterations caused by the infection.

  12. Characterization of three terpenoid glycosyltransferase genes in 'Valencia' sweet orange (Citrus sinensis L. Osbeck).

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    Fan, Jing; Chen, Chunxian; Yu, Qibin; Li, Zheng-Guo; Gmitter, Frederick G

    2010-10-01

    Three putative terpenoid UDP-glycosyltransferase (UGT) genes, designated CsUGT1, CsUGT2, and CsUGT3, were isolated and characterized in 'Valencia' sweet orange (Citrus sinensis L. Osbeck). CsUGT1 consisted of 1493 nucleotides with an open reading frame encoding 492 amino acids, CsUGT2 consisted of 1727 nucleotides encoding 504 amino acids, and CsUGT3 consisted of 1705 nucleotides encoding 468 amino acids. CsUGT3 had a 145 bp intron at 730-874, whereas CsUGT1 and CsUGT2 had none. The three deduced glycosyltransferase proteins had a highly conserved plant secondary product glycosyltransferase motif in the C terminus. Phylogenetic analysis showed that CsUGT1 and CsUGT3 were classified into group L of glycosyltransferase family 1, and CsUGT2 was classified into group D. Through Southern blotting analysis, CsUGT1 was found to have two copies in the sweet orange genome, whereas CsUGT2 and CsUGT3 had at least seven and nine copies, respectively. CsUGT1, CsUGT2, and CsUGT3 were constitutively expressed in leaf, flower, and fruit tissues. The results facilitate further investigation of the function of terpenoid glycosyltransferases in citrus and the biosynthesis of terpenoid glycosides in vitro.

  13. BIOSORPTION STUDIES OF CADMIUM (II IONS FROM AQUEOUS SOLUTIONS ONTO ORANGE RIND (CITRUS SINENSIS L. OSBECK

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    Satish A. Bhalerao

    2015-03-01

    Full Text Available The biosorption studies for effective removal of cadmium (II ions from aqueous solutions using orange rind (Citrus sinensis L. Osbeck, cost effective biosorbent, was carried out in batch system. FTIR analysis of biosorbent confirmed that carboxyl, hydroxyl, carbonyl group which was responsible for biosorption of cadmium (II ions. The SEM represents porous structure with surface area. The effects of operational factors including solution pH, biosorbent dose, initial cadmium (II ions concentration, contact time and temperature were studied. The optimum solution pH for cadmium (II ions biosorption by biosorbent was 7.0 with the optimal removal 80.30 %. The biosorbent dose 5 mg/ml was enough for optimal removal of 65.15 %. The biosorption process was relatively fast and equilibrium was achieved after 90 minutes of contact. The experimental equilibrium biosorption data were analysed by four widely used two-parameters Langmuir, Freundlich, Dubinin-Kaganer-Redushkevich (DKR and Temkin isotherm models. Langmuir isotherm model provided a better fit with the experimental data than Freundlich, Temkin and Dubinin-Kaganer-Redushkevich (DKR isotherm models by high correlation coefficient value (R2 = 0.911. The maximum adsorption capacity determined from Langmuir isotherm was found to be 83.33 mg/g of biosorbent. Simple kinetic models such as pseudo-first-order, pseudo-second-order, Elovich equation and Weber and Morris intra-particle diffusion rate equation were employed to determine the adsorption mechanism. Results clearly indicates that the pseudo-second-order kinetic model (R2 = 0.998 was found to be correlate the experimental data strongest than other three kinetic models and this suggests that chemical adsorption process was more dominant. Thermodynamic study revealed that the biosorption process was spontaneous, endothermic and increasing randomness of the solid solution interfaces. Orange rind (Citrus sinensis L. Osbeck was successfully used for the

  14. Honey bee attractants and pollination in sweet orange, Citrus sinensis (L. Osbeck, var. Pera-Rio

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    D. T. Malerbo-Souza

    2004-01-01

    Full Text Available This experiment studied the frequency and behavior of insects on sweet orange flowers, Citrus sinensis (L. Osbeck, their effect on fruit production (quantity and quality, nectar and pollen collection, and the effectiveness of different attractants. Over three consecutive years, the most frequent visitor to the flowers was Apis mellifera (Africanized. Flowers visited less than ten times showed low fructification. Fruit production was 35.30% greater in uncovered flowers. Fruit mean weight was much greater in uncovered (180.2g than in covered flowers (168.5g. Fruits from the covered were more acid (1.411g of citric acid/100ml of juice than the uncovered flowers (1.164g of citric acid/100ml of juice. The number of seeds per bud was higher in the uncovered (1 seed/bud than in the covered treatment (0.8 seed/bud. Bee-HereR, eugenol, geraniol, citral, and lemon grass extract, mainly diluted in water, were effective in attracting honeybees to orchards. However, these compounds were less effective when diluted in sugar syrup. The same products had variable attractiveness to honeybees in different years.

  15. Cardioprotective Activity of Ethanolic Extract of Citrus grandis (L. Osbeck Peel on Doxorubicin and Cyclophosphamide Induced Cardiotoxicity in Albino Rats

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    Samir Baniya

    2015-07-01

    Full Text Available The present study was designed to evaluate the cardioprotective potential of Citrus grandis (L. Osbeck (CGO peel extract in rats. Citrus grandis (L. Osbeck peel extract was evaluated for protection against cyclophosphamide (200 mg/kg body wt., i.p. and doxorubicin (15 mg/kg body wt., i.p. induced cardiotoxicity in male albino rats. Biomarkers like lactate dehydrogenase (LDH, alanine aminotransaminase (ALT, aspartate aminotransaminase (AST, alkaline phosphatase (ALP, total cholesterol (TC, triglyceride (TG and creatinine kinase (CK-MB along with heart weight index and antioxidant enzymes was considered to determine the cardioprotective property. Histopathological study was also carried out on heart of experimental animals. The CGO peel extract was found to contain alkaloids, flavonoids, steroids and triterpenoids, saponins, phenolic compounds and tannin as chemical constituents. Cyclophosphamide (CYP and doxorubicin (DOX treated groups exhibited significant increase in LDH, ALT, AST, ALP, TC, TG and CK-MB level and decrease in catalase (CAT, superoxide dimutase (SOD when compared to control group. Pretreatment with different doses of CGO significantly reduced the serum biomarkers and increased the tissue antioxidant level when compared to DOX and CYP alone treated groups. Moreover, treatment with CGO also improved CYP induced changes in histopathology of heart which may be due to its antioxidant property. The Citrus grandis (L. Osbeck exerted protective effect against CYP and DOX induced cardiotoxicity in rats, which may be due its lipid lowering and antioxidant properties. These findings might be helpful to understand the beneficial effects of CGO extract against myocardial injury although further study is needed to confirm its mechanism.

  16. Production of Transgenic Anliucheng Sweet Orange (Citrus sinensis Osbeck) with Xa21 Gene for Potential Canker Resistance

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    LI Ding-li; XIAO Xuan; and GUO Wen-wu

    2014-01-01

    Citrus canker, an epidemic quarantine disease caused by Xanthomonas axonopodis pv. citri, has brought a great damage in citrus production worldwide. Herein, a rice PRR (pattern recognition receptor) gene Xa21 together with GUS reporter gene and hygromycin phosphotransferase gene (HPT) was introduced into Anliucheng sweet orange (Citrus sinensis Osbeck) via Agrobacterium-mediated transformation of embryogenic callus. The transgenic calluses were screened on MT basal medium containing hygromycin (HYG) and detected by histochemical GUS staining. The transgenic plantlets were recovered through somatic embryogenesis pathway. The regenerated plantlets were accustomed to and maintained in the greenhouse. The transgene integration of recovered plantlets was identiifed by PCR and Southern blot hybridization. It showed that all the transgenic plantlets tested had undergone single copy integration, the expression of Xa21 in eight different transgenic lines detected by qRT-PCR can be divided into three grades, high for T5 and T6, middle for T4 and low for the rest. The tolerance to citrus canker disease of the three recovered transgenic lines T2, T4 and T6 was assessed by in vitro pin-puncture inoculation. The results showed that all the three transgenic lines conferred improved resistance to citrus canker bacterium infection and the T4 transgenic line displayed the highest resistance. The mechanism and feasibility of rice Xa21 in triggering innate immunity in citrus was brielfy discussed.

  17. Efeito de herbicidas residuais, aplicados por vários anos consecutivos, na distribuição do sistema radicular da laranjeira Natal (Citrus sinensis.(L. osbeck Effects of residual herbicides, applyed for several years son the root system distribution of 'Natal' orange (Citrus sinensis L. osbeck

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    R Victória Filho

    1985-12-01

    Full Text Available O presente trabalho foi desenvolvido com o objetivo de verificar a influência do uso contínuo de vários herbicidas residuais, sobre a distribuição das radicelas de laranjeiras Natal (Citrus sinensis (L. Osbeck enxertadas sobre limão Cravo (Cithus limonia Osbeck e plantadas em janeiro de 1970. O experimento foi instalado no pomar de produção da Faculdade de Ciências Agrárias e Veterinárias do Campus de Jaboticabal (UNESP, em Latossol Roxo distrófico, com 1,6% de matéria orgânica e 51% de argila. O delineamento experimental utilizado foi o de blocos ao acaso, dentro de um esquema fatorial 11x 3x2x2, com três repetições. Os tratamentos utilizados, com as respectivas doses em kg do i.a/ha foram: fluometuron a 4,2; simazíne a 4,8; atrazine a 4,8; bromacil a 3,2;bromacil (40% + diuron (40% a 4,8; bromacil (53,3% + diuron (27,6% a 4,8; terbacil a 3,2; oxadiazon a 1,5; dichlobenil a 3,0 e 6,0; além de uma testemunha capinada. O estudo das radicel as foi feito pelo "método do trado", tendo sido tomadas amostras de solo contendo radicelas, nas distâncias de 80, 160 e 240 cm do tronco, nas camadas de 0 a 15 cm e 15 a 30 cm de profundidade e em duas direções distintas (entrelinhas e entreplantas. Os resultados mostraram que as radicelas localizam-se superficialmente, com 70% delas nos primeiros 15 cm, do total encontrado na camada superficial de 0 a 30 cm de profundidade do solo. Cerca de 75% das radicelas situam-se até a distância de 160 cm do tronco. Na camada mais superficial do solo (0 a 15 cm, a quantidade de radicelas diminuiu de maneira acentuada, à medida que se afastou do tronco. Na camada de 15 a 30 cm de profundidade, ocorreu uma distribuição horizontal mais uniforme, comparada com a da camada de 0 a 15 cm. As radicelas das plantas do cultivar Natal distribuiram-se uniformemente, tanto na direção das plantas da linha vizinha, quanto nas das plantas de uma mesma linha, para o espaçamento de 7,0 m x 7,2 m. Não se

  18. Somatic hybridization between Citrus sinensis (L. Osbeck and C. grandis (L. Osbeck Hibridação somática entre Citrus sinensis (L. Osbeck e C. grandis (L. Osbeck

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    Marcia Cristina Calixto

    2004-07-01

    Full Text Available This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.O objetivo deste trabalho foi produzir híbridos somáticos entre laranjas doces e toranjas. Após fusão de protoplastos embriogênicos de laranja doce com protoplastos derivados de mesófilo foliar de toranjas, plantas foram regeneradas por embriogênese somática e aclimatizadas em casa de vegetação. Os híbridos laranja 'Hamlin' + toranja 'Indian Red' e laranja 'Hamlin' + toranja 'Singapura' foram confirmados pela morfologia foliar, contagem do número de cromossomos e análise molecular. Esses híbridos apresentam potencial para serem testados como porta-enxertos tolerantes a declínio, tristeza e doenças causadas por Phytophthora, bem como em programas de melhoramento de porta-enxertos.

  19. Transcriptional profiling of canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) constitutively overexpressing a spermidine synthase gene.

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    Fu, Xing-Zheng; Liu, Ji-Hong

    2013-01-01

    Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating diseases affecting the citrus industry worldwide. In our previous study, the canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) plants were produced via constitutively overexpressing a spermidine synthase. To unravel the molecular mechanisms underlying Xcc resistance of the transgenic plants, in the present study global transcriptional profiling was compared between untransformed line (WT) and the transgenic line (TG9) by hybridizing with Affymetrix Citrus GeneChip. In total, 666 differentially expressed genes (DEGs) were identified, 448 upregulated, and 218 downregulated. The DEGs were classified into 33 categories after Gene ontology (GO) annotation, in which 68 genes are in response to stimulus and involved in immune system process, 12 genes are related to cell wall, and 13 genes belong to transcription factors. These genes and those related to starch and sucrose metabolism, glutathione metabolism, biosynthesis of phenylpropanoids, and plant hormones were hypothesized to play major roles in the canker resistance of TG9. Semiquantitative RT-PCR analysis showed that the transcript levels of several candidate genes in TG9 were significantly higher than in WT both before and after Xcc inoculation, indicating their potential association with canker disease.

  20. Transcriptional Profiling of Canker-Resistant Transgenic Sweet Orange (Citrus sinensis Osbeck Constitutively Overexpressing a Spermidine Synthase Gene

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    Xing-Zheng Fu

    2013-01-01

    Full Text Available Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc is one of the most devastating diseases affecting the citrus industry worldwide. In our previous study, the canker-resistant transgenic sweet orange (Citrus sinensis Osbeck plants were produced via constitutively overexpressing a spermidine synthase. To unravel the molecular mechanisms underlying Xcc resistance of the transgenic plants, in the present study global transcriptional profiling was compared between untransformed line (WT and the transgenic line (TG9 by hybridizing with Affymetrix Citrus GeneChip. In total, 666 differentially expressed genes (DEGs were identified, 448 upregulated, and 218 downregulated. The DEGs were classified into 33 categories after Gene ontology (GO annotation, in which 68 genes are in response to stimulus and involved in immune system process, 12 genes are related to cell wall, and 13 genes belong to transcription factors. These genes and those related to starch and sucrose metabolism, glutathione metabolism, biosynthesis of phenylpropanoids, and plant hormones were hypothesized to play major roles in the canker resistance of TG9. Semiquantitative RT-PCR analysis showed that the transcript levels of several candidate genes in TG9 were significantly higher than in WT both before and after Xcc inoculation, indicating their potential association with canker disease.

  1. Uso contínuo de herbicidas em citrus (Citrus sinensis L. osbeck : II. Efeitos no desenvolvimento, produção e qualidade dos frutos Continuos use of herbicides in citros (Citrus sinensis (L. osbeck: II - ffects ondevelopment, yield and fruit quality

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    Ricardo Victoria Filho

    1991-01-01

    Full Text Available A presente pesquisa foi conduzida no município de Conchal - SP, Brasil, em um Latossol Vermelho Amarelo com 1,75% de matéria orgânica com o objetivo de veri fic ar o efeito do uso cont inuo dos principais herbicidas no desenvolvimento, produção e qualidade dos frutos em um pomar de laranja 'Pera' Citrus sinensis (L. Osbeck, en xertada sobr e limão cravo (Citrus lionia Osbeck. O delineamento experimental adotado foi o de blocos ao acaso, com 12 tratamentos e 4 repetições. Os tratamentos utilizados com as respectivas doses do i. a. em kg/h a foram: terbacil a 3,2; simazine a 4,0; ametryne + secbumetone 4,5; dichlobenil a5,0 ; diuron a3,2 ; bromacila 3,2; bromacil + diuron a 3,2; paraquat a 0,6; glyphosate a 1,61 e MSMA a 1,77 alem de uma testemunha que recebia uma capina anualmente, e outra que era capinada sempre que a cobertura pelas plantas daninhas atingia 25% da área da parcela. O pomar foi plantado em meio/ 75 e a 1a aplicação dos herbicidas foi realizada em outubro de 1977. As parcelas continham 4 plantas em uma area de 3,0 x 18,0 m (54 m2. A última aplic ação foi realizada em 1992. O efeito no desenvolvimento foi feito pela medida do diâmetro do caule a 10 m acima do ponto de enxertia, pelo diâmetro da copa na altura mediana e pela altura das plantas. Todas essas medidas eram realizadas 2 vezes por ano. Para avaliação da produção, eram colhidos os frutos de quatro plantas por parcela. A qualidade dos frutos foi avaliada através das medidas do diâmetro longitudinal e transversal, albedo, peso e número de sementes, peso médio dos frutos, % de suco, % de sólidos solúveis e % de acidez. Pelos dados obtidos , verifica - se que não houve influência dos herbicidas no desenvolvimento das plantas de citros, e na produção. As influencias na qualidade dos frutos foram mínimas, e dependeram do ano de amostragem.The research reported in this paper was conducted at the Conchal county in Sao Paulo State, Brazil, in a Red

  2. The Composition, Antioxidant and Antibacterial Activities of Cold-Pressed and Distilled Essential Oils of Citrus paradisi and Citrus grandis (L. Osbeck

    Directory of Open Access Journals (Sweden)

    Ming-Chiu Ou

    2015-01-01

    Full Text Available The chemical composition and functional activities of cold-pressed and water distilled peel essential oils of Citrus paradisi (C. paradisi and Citrus grandis (L. Osbeck (C. grandis were investigated in present study. Yields of cold-pressed oils were much higher than those of distilled oils. Limonene was the primary ingredient of essential oils of C. paradisi (cold 92.83%; distilled 96.06% and C. grandis (cold 32.63%; distilled 55.74%. In addition, C. grandis oils obtained were rich in oxygenated or nitrogenated compounds which may be involved in reducing cardiovascular diseases or enhancing sleep effectiveness. The order of free radical scavenging activities of 4 citrus oils was distilled C. paradisi oil > cold-pressed C. paradisi oil > distilled C. grandis oil > cold-pressed C. grandis oil. Cold-pressed C. grandis oil exhibited the lowest activity in all antioxidative assays. The order of antimicrobial activities of 4 citrus oils was distilled C. grandis oil, cold-pressed C. paradisi oil > distilled C. paradisi oil > cold-pressed C. paradisi oil. Surprisingly, distilled C. grandis oil exhibited better antimicrobial activities than distilled C. paradisi oil, especially against Escherichia coli and Salmonella enterica subsp. The results also indicated that the antimicrobial activities of essential oils may not relate to their antioxidative activities.

  3. The Composition, Antioxidant and Antibacterial Activities of Cold-Pressed and Distilled Essential Oils of Citrus paradisi and Citrus grandis (L.) Osbeck

    Science.gov (United States)

    Ou, Ming-Chiu; Liu, Yi-Hsin; Sun, Yung-Wei; Chan, Chin-Feng

    2015-01-01

    The chemical composition and functional activities of cold-pressed and water distilled peel essential oils of Citrus paradisi (C. paradisi) and Citrus grandis (L.) Osbeck (C. grandis) were investigated in present study. Yields of cold-pressed oils were much higher than those of distilled oils. Limonene was the primary ingredient of essential oils of C. paradisi (cold 92.83%; distilled 96.06%) and C. grandis (cold 32.63%; distilled 55.74%). In addition, C. grandis oils obtained were rich in oxygenated or nitrogenated compounds which may be involved in reducing cardiovascular diseases or enhancing sleep effectiveness. The order of free radical scavenging activities of 4 citrus oils was distilled C. paradisi oil > cold-pressed C. paradisi oil > distilled C. grandis oil > cold-pressed C. grandis oil. Cold-pressed C. grandis oil exhibited the lowest activity in all antioxidative assays. The order of antimicrobial activities of 4 citrus oils was distilled C. grandis oil, cold-pressed C. paradisi oil > distilled C. paradisi oil > cold-pressed C. paradisi oil. Surprisingly, distilled C. grandis oil exhibited better antimicrobial activities than distilled C. paradisi oil, especially against Escherichia coli and Salmonella enterica subsp. The results also indicated that the antimicrobial activities of essential oils may not relate to their antioxidative activities. PMID:26681970

  4. Production of intergeneric somatic hybrids between round kumquat (Fortunella japonica Swingle) and 'Morita navel' orange (Citrus sinensis Osbeck).

    Science.gov (United States)

    Takami, K; Matsumara, A; Yahata, M; Imayama, T; Kunitake, H; Komatsu, H

    2004-08-01

    Intergeneric somatic hybrids between embryogenic callus-derived protoplasts of round kumquat (Fortunella japonica Swingle) and 'Morita navel' orange (Citrus sinensis Osbeck) were produced by electrofusion. Among the eight different fusion strains obtained, six showed normal morphology, whereas the remaining two showed malformation. All the regenerated plants were intermediate in leaf morphology and had thick and round leaves, which are typical characteristics of polyploids. Ploidy analyses by flow cytometry and chromosome counting in root-tip cells revealed that these plants are amphidiploid (2n=4x=36). Hybridity of the fusion products was confirmed by random amplified polymorphic DNA and cleaved amplified polymorphic sequence (CAPS) analyses. Furthermore, analyses of chloroplast (cp) and mitochondrial (mt) DNA by CAPS showed that these somatic hybrids contained cp- and mt-DNA of round kumquat without recombination in the regions analyzed. PMID:14997338

  5. In vitro Plant Regeneration from the Mature Tissue of Navel Orange (Citrus sinensis L. Osbeck) by Direct Organogenesis

    Institute of Scientific and Technical Information of China (English)

    HUANG Jia-quan; YIN Li-yan; YANG Xiao-hong; SUN Zhong-hai

    2005-01-01

    An efficient in vitro regeneration system by direct organogenesis from mature nodal and internodal stem segments of Newhall navel orange (Citrus sinensis L. Osbeck) was developed. Illuminating conditions together with plant growth regulators affected the adventitious bud regeneration frequency and efficiency. The initial 15 d darkness inoculation is beneficial for the adventitious bud regeneration. The highest regeneration frequency (85.2%) and bud formation efficiency (3.7 per responsive internodal stem segment) were obtained in the media supplemented with 1.0 mg L-1 BAP and 0.5 mg L-1 NAA. ABA at 0.2 mg L-1 positively affected the bud formation efficiency, which amounted to 8.5 buds per internodal segment in the presence of BAP at 1.0 mg L-1. The adventitious shoots successfully rooted and were transferred to the soil.

  6. Construction and Characterization of a cDNA Library from the Pulp of Cara Cara Navel Orange (Citrus sinensis Osbeck)

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A cDNA library was constructed and characterized from the pulp of Cara Cara navel orange (Citrus sinensis Osbeck) at different stages of ripening. Tittering results revealed that approximately 5.086x105 independent clones were included in this library. Electrophoresis gel results of 15 randomly selected clones revealed that the size of the insertion fragments ranged from 400 bp to 2 kb, with an average size of 900 bp. Sequencing results of 150 randomly picked clones showed that the recombination rate was 94%. During subsequent sequence analysis, 41 of 139 clones failed to be identified and the amino sequence of 71 clones shared less than 30% identity with related plants in GenBank. Of 27 clones whose amino sequences shared more than 60% identity with other related plants in GenBank, 17 clones showed an 80% identity with the corresponding candidate genes of citrus. The clone recognized as the type Ⅲ metallothionein-like (MT) gene was observed to occur 13 times, indicating that the protein may play an important role in fruit development and ripening.

  7. 不同土壤对白黎檬光合特性的影响%Effects of Different Soils on Photosynthetic Characteristics of Citrus limonia

    Institute of Scientific and Technical Information of China (English)

    吴素芳; 樊卫国

    2012-01-01

    To explore the adaptability for C. limonia to different soils, the seedlings were planted separately in pots with metamorphic rocks cane yellow soil, quaternary red clay cane yellow soil, yellow calcareous soil, brown calcareous soil and black calcareous soil. Photosynthetic characteristics of C. limonia in five different soils were studied, and the relation between soil nutrient and photosynthetic rate(Pn) of C. li-monia was analyzed. The results showed that; (i) Pn of C. limonia in black calcareous soil was the highest[8.91 μmol CO2/(m2 o s) ], chlorophyll(Chl) content was also the highest(2.24 mg/g‐1, and the adaptability range to light intensity was the widest; Pn and Chi content of C. limonia in yellow calcareous soil took the second place, while Pn and Chi content in quaternary red clay cane yellow soil were both the lowest[ 1.17 μ.molC02/(m2 · g) and 0.73 mg/g]. The effects of alkaline hydrolysis N, available Fe and available Cu in different soils on Pn of C. limonia were significant, while other mineral elements in different soils on Pn of C. limonia were not significant. (ii) Under the same climate condition, the adaptability changes of Pn content, Chl content and basic light requirement characteristics of C. limonia occurred on different soils, and black calcareous soil was the most conducive to the improvement of light utilization efficiency of C. limonia.%为了探索白黎檬对不同土壤的适应性,采用盆栽土培试验研究了白黎檬在变质岩母质黄壤、第4纪红色粘土母质黄壤、黄色石灰土、棕色石灰土和黑色石灰土5种土壤上的光合特性,分析土壤养分含量与白黎檬光合速率(Pn)的关系.结果表明:①黑色石灰土上白黎檬的Pn最大,为8.91 μmolCO2/(m2·s)叶绿素(Chl)含量最高,为2.24 mg/g,对光照强度的适应范围最大;黄色石灰土上白黎檬的Pn及Chl含量都仅次于黑色石灰土,而第4纪红色粘土母质黄壤上白黎檬的Pn最小,为1.17 μmolCO2/(m2

  8. Visitantes florais e produção de frutos em cultura de laranja ( Citrus sinensis L. Osbeck = Floral visitors and fruit production on sweet orange crop ( Citrus sinensis L. Osbeck

    Directory of Open Access Journals (Sweden)

    Lourdes Maria Gamito

    2006-10-01

    Full Text Available O presente experimento foi realizado em florada de laranja (Citrus sinensis L. Osbeck, variedade Pera-Rio, com os objetivos de estudar os insetos visitantes nas flores d e laranjeira, o seu comportamento nas flores, o tipo de coleta efetuada e o efeito dessas visitas na produção de frutos, em quantidade e qualidade. Os dados de freqüência foram obtidos por contagem nos primeiros 10 minutos de cada horário, das 8h às 18h, em três dias distintos, percorrendo-se as linhas da cultura. O comportamento forrageiro de cada espécie de inseto foi avaliado através de observações visuais, no decorrer do dia, no período experimental. Os insetos observados foram abelhas africanizadas Apis mellifera, Trigona spinipes e Tetragonisca angustula. As abelhas A. mellifera foram os visitantes florais maisfreqüentes e preferiram coletar néctar comparado ao pólen. Os botões florais descobertos produziram mais frutos que os botões florais cobertos. Os frutos decorrentes do tratamento coberto foram menores, mais ácidos e com menor quantidade de vitamina C que os frutos do tratamento descoberto. The present experiment was carried out in flowerage of sweet orange(Citrus sinensis L. Osbeck, Pera-rio variety, to study the insects involv ed in pollination, their behaviour in the flower (nectar or pollen collection and the effect of the pollination on fruit production (quantity and quality. More frequent insects were recorded daily (counted during ten minutes, every hour from 8:00 a.m. to 6:00 p.m., with three replications. The forage behaviour and nectar and/or pollen collect was also observed. The insect visitors onflowers were Africanized honey bee Apis mellifera, followed by stingless bees Trigona spinipes and Tetragonisca angustula. A. mellifera were the most frequent visitors and preferred to collectnectar than pollen. The uncovered flowers -buds produced more fruits than the covered ones. Another observation was that fruits derived from covered

  9. OBTENÇÃO DE PLANTAS DE LIMÃO CRAVO (Citrus limonia Osbeck E TANGERINA CLEÓPATRA (Citrus reshni Hort. A PARTIR DO CULTIVO DE PROTOPLASTOS DE SUSPENSÃO CELULAR PLANT REGENERATION OF 'RANGPUR' LIME (Citrus limonia Osbeck AND 'CLEÓPATRA' MANDARIN (Citrus reshni Hort. THROUGH PROTOPLASTS OF CELL SUSPENSION

    Directory of Open Access Journals (Sweden)

    Rodrigo Rocha Latado

    1999-01-01

    Full Text Available Este trabalho descreve uma metodologia para a regeneração de plantas de tangerina 'Cleópatra' e limão 'Cravo', a partir do cultivo de protoplastos de suspensão celular. Para tal, calos nucelares foram induzidos em meio contendo BAP e cultivados em meio sem reguladores de crescimento. Protoplastos foram isolados de suspensões celulares e cultivados em gotas de agarose, com densidade de 2 X 105 protoplastos.ml-1. O meio MT, contendo ácido giberélico e água de coco, foi eficiente na germinação de embriões somáticos. Os métodos de aclimatação de plantas testados apresentaram baixa eficiência. Como resultado final, 17 plantas adaptadas de tangerina e 8 de limão foram obtidas.The present research describes the regeneration of 'Cleópatra' mandarin and 'Rangpur' lime plants from cell suspension protoplasts. Nucelar calli were induced on a medium containing BAP and maintained on growth regulator free medium. Protoplasts were isolated from embryogenic suspension and plated at a concentration of 2 X 105 protoplasts.ml-1, on agarose droplets. The MT medium with gibberellic acid and coconut water was efficient to stimulate somatic embryo conversion. Rooted plants acclimation had low efficiency. Seventeen mandarin plants and eight lime plants were obtained.

  10. The Analysis of MADS box Genes Expressing in the Citrus grandis Osbeck cv.%柚(Citrus grandis Osbeck cv.)MADS box基因的表达分析

    Institute of Scientific and Technical Information of China (English)

    张敬虎; 潘一山; 王少峰; 邹金美; 陈羡德; 林莹; 王玉玲; 蓝炎阳

    2015-01-01

    This paper is concerning the MADS box gene family from the fruitlet (less than 5 days) and the yang leaf of two kinds of pomelo breeding (Citrus grandis Osbeck cv.) using the De novo transcriptomics method. The fruitlet and the yang leaf from the Early Mature Dragon Pomelo (which was taken as mutant group)and the Guangxi Sweet Pomelo (which was taken as reference group)were taken as experiment materials, by RNA extraction, reverse transcription, sequencing and assembly Unigene sequences. The MADS box transcription factor family gene annotation results were retrieved from the Nr, Swiss Prot, KEGG and COG the four databases. Adopting digital gene expression label (DGE) technology, the gene transcription abundance in the sample was taken as RPKM value, that the differentially expressed genes (DEGs) were identified by comparing the different of the RPKM value between the mutant group and reference group, with the duplication≥2. There were 28 MADS box genes were annotated from the fruitlet and the yang leaf of the Early Mature Dragon Pomelo and the Guangxi Sweet Pomelo. There were 25 MADS box genes expressed in the fruitlet from the Early Mature Dragon Pomelo (FE) and the Guangxi Sweet Pomelo (FL), and sixs of them were DEGs. Meanwhile, There were 27 MADS box genes expressed in the leaf from the Early Mature Dragon Pomelo (LE) and the Guangxi Sweet Pomelo (LL), and sevens of them were DEGs. The 28 MADS box gene family was reported eighteens of them in Citrus clementina and twenties of them in Citrus sinensis, respectively, and fives of them have reported neither in Citrus clementina nor in Citrus sinensis, and have not found in the Citrus's other breeding.%以De novo转录组学方法分析柚(Citrus grandis Osbeck cv.)MADS box基因家族的基因.以特早熟龙柚(变异型)和琯溪蜜柚(参照型)嫩果(不超过5天)和新叶为材料,经过RNA提取、反转录、测序、组装得到Unigene序列. Unigene序列在 Nr、Swiss-Prot、KEGG 和 COG 四

  11. Supercritical Carbon Dioxide Extraction of Flavonoids from Pomelo (Citrus grandis (L. Osbeck Peel and Their Antioxidant Activity

    Directory of Open Access Journals (Sweden)

    Qiao-Mei Ru

    2012-10-01

    Full Text Available Supercritical carbon dioxide (SC-CO2 extraction of flavonoids from pomelo (Citrus grandis (L. Osbeck peel and their antioxidant activity were investigated. Box-Behnken design combined with response surface methodology was employed to maximize the extraction yield of flavonoids. Correlation analysis of the mathematical-regression model indicated that a quadratic polynomial model could be used to optimize the SC-CO2 extraction of flavonoids. The optimal conditions for obtaining the highest extraction yield of flavonoids from pomelo peel were a temperature of 80 °C, a pressure of 39 MPa and a static extraction time of 49 min in the presence of 85% ethanol as modifier. Under these conditions, the experimental yield was 2.37%, which matched positively with the value predicted by the model. Furthermore, flavonoids obtained by SC-CO2 extraction showed a higher scavenging activity on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS radicals than those obtained by conventional solvent extraction (CSE. Therefore, SC-CO2 extraction can be considered as a suitable technique for the obtainment of flavonoids from pomelo peel.

  12. 沙田柚活性成分的提取及其生理功能的研究进展%Development of Extraction and Physiological Activities of Active Substance from Citrus Graudis Osbeck

    Institute of Scientific and Technical Information of China (English)

    王标诗; 吴艺华; 杜建中; 江敏; 金蓓; 胡小军; 彭元怀

    2014-01-01

    论述了沙田柚活性成分(如精油、果胶、甙类物质、生物碱和水溶性多糖等)的提取方法及其活性成分的生理功能(抗菌、抗氧化、降血脂等作用),并对其发展前景进行展望,以期为沙田柚的高值化综合开发利用提供参考。%This review focused on extraction of active ingredients (such as essential oils, pectin, glycosides, alkaloids and water-soluble polysaccharides) from Citrus Graudis Osbeck and their physiological function (antibacterial activity,antioxidant activity and hypolipidemic)and the developing prospects of Citrus Graudis Osbeck were also discussed in the future, which is helpful to comprehensive development and utilization of Citrus Graudis Osbeck.

  13. 柚(Citrus grandis Osbeck cv.)MADS box基因的表达分析

    Institute of Scientific and Technical Information of China (English)

    张敬虎[1; 潘一山[1; 王少峰[2; 邹金美[1; 陈羡德[1; 林莹[1; 王玉玲[1; 蓝炎阳[2

    2015-01-01

    以De novo转录组学方法分析柚(Citrus grandis Osbeck cv.)MADS box基因家族的基因.以特早熟龙柚(变异型)和琯溪蜜柚(参照型)嫩果(不超过5天)和新叶为材料,经过RNA提取、反转录、测序、组装得到Unigene序列.Unigene序列在Nr、Swiss-Prot、KEGG和COG四大数据库中进行比对,获得MADS box转录因子家族基因注释结果.采用数字基因表达标签(digital gene expression tag,DGE)技术,对样本中基因转录丰度以RPKM值表示,比较处理组和对照组的RPKM值,以倍性变化≥2确定差异表达基因(differentially expressed genes,DEGs).从特早熟龙柚和琯溪蜜柚嫩果和叶片中,总共注释到28个MADS box基因家族基因.在龙柚嫩果(FE)和蜜柚嫩果(FL)中表达的有25个,其中有6个属于差异表达基因.在龙柚叶片(LE)和蜜柚叶片(LL)中表达的有27个,其中有7个属于差异表达基因.注释到的28个MADS box基因家族基因在克莱门特柚(Citrus clementina)和中国甜橙(Citrus sinensis)报道过的分别有18个和20个,其中有5个基因既在中国甜橙和克莱门特柚都没有报道过,也没有发现在其它Citrus物种报道过.

  14. Época da indução e evocação floral em Citrus sinensis (L. Osbeck cv. Pêra Rio The flower induction and evocation period in Citrus sinensis (L. Osbeck cv. Pêra Rio

    Directory of Open Access Journals (Sweden)

    Iara Alvarenga Mesquita Pereira

    2003-10-01

    Full Text Available A produção do estímulo floral (indução e emissão das primeiras sépalas, na gema floralmente determinada (evocação floral em Citrus sinensis (L. Osbeck cv. Pêra Rio pôde ser acompanhada pelas variações anatômicas nos meristemas apicais e axilares. Com objetivo de determinar a época na qual ocorre a indução e evocação floral, cortes longitudinais de gemas apicais e axilares, corados com pironina Y-methylgreen foram efetuados em períodos regulares de maio a agosto. Através das mudanças no formato da gema, principalmente diâmetro, que aumentou com a indução, foi possível determinar a época do ano na qual gemas vegetativas são induzidas a florescerem. Foram detectadas variações nos diâmetros das gemas no período de início da morfogênese floral (meados até o final de julho. A medição do diâmetro das gemas mostra-se um procedimento eficiente para acompanhar os fenômenos decorrentes da indução, evocação e morfogênese florais. Gemas vegetativas apresentam diâmetro médio de 100µm, com túnica composta por três camadas, de formato cônico, recobrindo o corpo, ao passo que a reprodutiva é mais achatada, após a iniciação do primeiro primórdio de sépala, exibindo diâmetro médio de 200µm. Coloração com pironina Y-methylgreen proporciona a captação do fenômeno da evocação floral, pela detecção de regiões mais concentradas em RNA, nas zonas periféricas das gemas; alteração bioquímica esta que precede a emissão das sépalas.The research aims to determine the time in which the inflorescence induction and evocation occurs. The production of inflorescence stimulus (induction and emission of first sepals in a bud determined as a flower bud (floral evocation in Citrus sinensis (L. Osbeck cv. Pêra Rio could be observed through the anatomic variations of the apical and lateral meristems. Longitudinal sections, stained with pironina Y-methylgreen were done in regular periods from May to August. The

  15. Molecular Characterization and Genetic Diversity Analysis of Sweet Orange Citrus sinensis L. Osbeck Cultivars in Iraq Using RAPD Markers

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    Ali Saeed Atiyah AL-Janabi

    2016-03-01

    Full Text Available Sweet orange (Citrus sinensis L. Osbeck is one of the most important commercially cultivated fruit crops of Citrus. Genetic diversity and inter-relationship among 5 cultivars (Indian, Iraqi, Japanese, Syrian, Egyptian of C. sinensis were analyzed based on RAPD markers. Six primers generated reproducible and easily storable RAPD profiles with a number of amplified DNA fragments ranging from 6 to 14 fragment bands. The total number of amplicons detected was 51, including 14 fragments unique bands with average reached 2.8 fragments ̸ primers. While the number of polymorphic ranged from 0 to 8 with an average reached 4.4 fragments ̸ primers with the polymorphic percentage ranged from 0% to 57.1%. While the number of monomorphic ranged from 2 to 5 fragment bands and was total of the monomorphic 15 fragments with an average reached 3 fragments ̸ primers with the monomorphic percentage was 14.2 % to 83.3%. A maximum numbers of amplicons was amplified with primer OPS-238 reached 14 fragments while the minimum number of fragments was amplified with primer OPS-253 reached 6 fragments. The highest number of polymorphic bands reached 8 fragments was obtained with primer OPS-238 with high percentage 57.1%, while the highest number of monomorphic bands reached 5 fragments with high percentage 83.3% was obtained with primer OPS-253. RAPD markers detected genetic distance and similarity, amaximum genetic distance value was observed between Japanese (Jap and Syrian (Syr cultivars reached 0.530 with less similarity value reached 47%, a minimum genetic distance value was observed between sweet Iraqi (Irq and Indian (Ind cultivars reached 0.239 with high similarity value reached 76.1%. The similarity matrices were employed in the cluster analysis to generate a dendrogram using the UPGMA method. The cluster tree analysis showed that the sweet orange cultivars were broadly divided into two main groups A and B with similarity reached 50%. A group including

  16. Collection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).

    Science.gov (United States)

    Hijaz, Faraj; Killiny, Nabil

    2014-01-01

    Through utilizing the nutrient-rich phloem sap, sap feeding insects such as psyllids, leafhoppers, and aphids can transmit many phloem-restricted pathogens. On the other hand, multiplication of phloem-limited, uncultivated bacteria such as Candidatus Liberibacter asiaticus (CLas) inside the phloem of citrus indicates that the sap contains all the essential nutrients needed for the pathogen growth. The phloem sap composition of many plants has been studied; however, to our knowledge, there is no available data about citrus phloem sap. In this study, we identified and quantified the chemical components of phloem sap from pineapple sweet orange. Two approaches (EDTA enhanced exudation and centrifugation) were used to collect phloem sap. The collected sap was derivatized with methyl chloroformate (MCF), N-methyl-N- [tert-butyl dimethylsilyl]-trifluroacetamide (MTBSTFA), or trimethylsilyl (TMS) and analyzed with GC-MS revealing 20 amino acids and 8 sugars. Proline, the most abundant amino acid, composed more than 60% of the total amino acids. Tryptophan, tyrosine, leucine, isoleucine, and valine, which are considered essential for phloem sap-sucking insects, were also detected. Sucrose, glucose, fructose, and inositol were the most predominant sugars. In addition, seven organic acids including succinic, fumaric, malic, maleic, threonic, citric, and quinic were detected. All compounds detected in the EDTA-enhanced exudate were also detected in the pure phloem sap using centrifugation. The centrifugation technique allowed estimating the concentration of metabolites. This information expands our knowledge about the nutrition requirement for citrus phloem-limited bacterial pathogen and their vectors, and can help define suitable artificial media to culture them.

  17. Phytoene Synthase Gene Cloning from Citrus sinensis Osbeck cv.Cara Cara and Its Prokaryotic Expression

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jian-cheng; TAO Neng-guo; TONG Zhu; DENG Xiu-xin

    2008-01-01

    Using the mRNA from the fruit of Cara Cara as the template,the cDNA of phytoene synthase(PSY)gene was amplified by reverse transcription polymerse chain reaction(RT-PCR).Sequence analysis indicated that the eDNA was of 1 520 bp,which had an open reading frame of 1 308 bp and encoded a protein of 436 amino acids.The homology analysis showed that PSY of Cara Cara shared high similarities of nucleotides and deduced amino acids with those in other plants up to more than 75 and 70%,respectively.A putative signal transit peptide for plastid targeting was found in the N-terminal region of PSY.The mature forms of PSY included a transmembrane(TM) domain.The recombinant plasmid pET-CitPSY was constructed by subeloning the full coding sequence of PSY eDNA into pET-28(+).After transformation of E.coil BL21 and induced by 1 mmol L-1 isopropyl-a-D-thiogalacropyranoside(IPTG),the fusion protein(6×His-PSY)with 52 kD was produced at a high level by prokaryotic expression system.The results of Western blot demonstrated that the fusion protein(6xHis-PSY)could be recognized by anti-6×His monoclonal antibody.The study could establish a basis for molecular improvement of Citrus fruit colors.

  18. Inotropic effect of Citrus sinensis (L. Osbeck leaf extracts on the guinea pig atrium

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    Oliveira E.D.

    2005-01-01

    Full Text Available The objective of the present investigation was to determine the contractile effect of crude and acetone leaf extracts of Citrus sinensis (L. Osb. on mammalian myocardium. Crude leaf extracts have been used in folk medicine to treat neurological disorders. Some flavonoids isolated from this plant presented a positive inotropic effect on myocardium. This motivated us to test the extracts on the atria of guinea pigs of both sexes (300-500 g and surprisingly we observed inotropic depression instead of an increase in force. The maximum effect of the crude extract was 79.4 ± 8.1% of the control force amplitude (N = 5 hearts, 10 trials, 27 ± 0.1ºC, stimulus: 2 Hz, 400 V, 0.5 ms. The EC50 for crude, ethanol, acetic, aqueous, and acetone extracts was 300, 300, 600, 1000, and 140 µg/ml, respectively, with a Hill constant of 1.8, 2.0, 2.5, 2.0, and 1.4, respectively. Blockade of cholinergic, beta-adrenergic, or opioid membrane receptors with 1.5 µM atropine sulfate, 1 µM propranolol, and 10 µM naloxone, respectively, did not change the effect of the crude extract. The acetone extract abolished the Bowditch positive staircase phenomenon (N = 5 hearts, 10 trials, 27 ± 0.1ºC, suggesting a possible reduction of the calcium inward current, and also promoted the so-called Woodworth phenomenon. The effect was concentration-dependent and indicated the existence of another inhibitory contractile mechanism such as the simultaneous activation of some of the membrane potassium channels reducing the myocardial action potential duration and further decreasing the cellular calcium entry.

  19. Composition of Citrus sinensis (L.) Osbeck cv «Maltaise demi-sanguine» juice. A comparison between organic and conventional farming.

    Science.gov (United States)

    Letaief, Hend; Zemni, Hassen; Mliki, Ahmed; Chebil, Samir

    2016-03-01

    Juices from conventionally and organically grown Citrus sinensis (L.) Osbeck cv. Maltaise demi-sanguine blood orange were investigated for quality parameters and antioxidant capacity. This blood orange variety is particularly rich in linoleic, linolenic acids, vitamin C and phenolic compounds. The quantitative determination of these compounds in cv. Maltaise demi-sanguine juice produced under conventional and organic agricultural practices revealed significant differences. The organically grown fruits contained more hesperidin and total fatty acids amounts as well as a higher sugar content and a lower acidity. Conventionally-grown fruit was found to have an increase in antioxidant capacity. In addition to having higher antioxidant activity conventionally-grown fruit had an observed increase in the concentration of phenolic acids and most flavonoids. The results of this study indicated that organically-grown Maltaise demi-sanguine juice contained an increased concentration of hesperidin which has been observed to possess biological activities associated with a healthy life. PMID:26471557

  20. First evidence of C- and O-glycosyl flavone in blood orange (Citrus sinensis (L.) Osbeck) juice and their influence on antioxidant properties.

    Science.gov (United States)

    Barreca, Davide; Bellocco, Ersilia; Leuzzi, Ugo; Gattuso, Giuseppe

    2014-04-15

    RP-LC-DAD-ESI-MS-MS separation/identification protocol has been employed for the identification and characterisation of nine C- and O-glycosyl flavonoids in Moro (Citrus sinensis (L.) Osbeck) juice grown in Southern Italy. For the first time we reported the presence of five C-glycosyl flavones (lucenin-2, vicenin-2, stellarin-2, lucenin-2 4'-methyl ether and scoparin), a 3-hydroxy-3-methylglutaryl glycosyl flavonol (3-hydroxy-3-methylglutaryl glycosyl quercetin) and a flavone O-glycosides (chrysoeriol 7-O-neoesperidoside). Moreover, the influence of the identified C- and O-glycosyl flavonoids on the total antioxidant activity of crude juice has been evaluated on the basis of its ability to scavenge DPPH•, OH• and ABTS•+ radicals and to reduce iron.

  1. C- and O-glycosyl flavonoids in Sanguinello and Tarocco blood orange (Citrus sinensis (L.) Osbeck) juice: Identification and influence on antioxidant properties and acetylcholinesterase activity.

    Science.gov (United States)

    Barreca, Davide; Gattuso, Giuseppe; Laganà, Giuseppina; Leuzzi, Ugo; Bellocco, Ersilia

    2016-04-01

    Sanguinello and Tarocco are the blood orange (Citrus sinensis (L.) Osbeck) cultivars most diffused worldwide. Reversed phase liquid chromatography coupled with MS-MS analysis showed that these two varieties have a similar chromatographic pattern, characterised by the presence of C- and O-glycosyl flavonoids. Of the two, Sanguinello was found to be far richer in flavonoids than Tarocco. In the juices, twelve individual components were identified for the first time, namely, four C-glycosyl flavones (lucenin-2, vicenin-2, stellarin-2, lucenin-2 4'-methyl ether and scoparin), three flavonol derivatives (quercetin-3-O-(2-rhamnosyl)-rutinoside, quercetin-3-O-hexoside, quercetin 3-hydroxy-3-methylglutaryl-glycoside), an O-triglycosyl flavanone (narirutin 4'-O-glucoside) and a flavone O-glycosides (chrysoeriol 7-O-neoesperidoside). Moreover, the influence of the identified C- and O-glycosyl flavonoids on the antioxidant and acetylcholinesterase activity of these juices has been evaluated.

  2. Composition of Citrus sinensis (L.) Osbeck cv «Maltaise demi-sanguine» juice. A comparison between organic and conventional farming.

    Science.gov (United States)

    Letaief, Hend; Zemni, Hassen; Mliki, Ahmed; Chebil, Samir

    2016-03-01

    Juices from conventionally and organically grown Citrus sinensis (L.) Osbeck cv. Maltaise demi-sanguine blood orange were investigated for quality parameters and antioxidant capacity. This blood orange variety is particularly rich in linoleic, linolenic acids, vitamin C and phenolic compounds. The quantitative determination of these compounds in cv. Maltaise demi-sanguine juice produced under conventional and organic agricultural practices revealed significant differences. The organically grown fruits contained more hesperidin and total fatty acids amounts as well as a higher sugar content and a lower acidity. Conventionally-grown fruit was found to have an increase in antioxidant capacity. In addition to having higher antioxidant activity conventionally-grown fruit had an observed increase in the concentration of phenolic acids and most flavonoids. The results of this study indicated that organically-grown Maltaise demi-sanguine juice contained an increased concentration of hesperidin which has been observed to possess biological activities associated with a healthy life.

  3. Populational fluctuation of vectors of Xylella fastidiosa, wells in sweet orange [Citrus sinensis (L. Osbeck] varieties of northwest Paraná State, Brazil

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    Rúbia de Oliveira Molina

    2010-06-01

    Full Text Available The aim of the present study was to assess the population flutuation of the sharpshooters species subfamily Cicadellinae belonging to the tribes Cicadellini and Proconiini, in sweet orange [Citrus sinensis( L. Osbeck] commercial orchards of the northwest region of Paraná State , Brazil. Samplings were carried out the employing every time 24 yellow sticky cards. Identification of the species showed that the most representative were Dilobopterus costalimai of the Cicadellini tribe and Acrogonia citrina of the Proconiini tribe.A Clorose variegada dos citros (CVC é uma importante doença que ocorre nos citros, cujo agente causal é a bactéria Xylella fastidiosa, Wells. A bactéria depende, obrigatoriamente, de insetos vetores para sua disseminação, que são as cigarrinhas sugadoras do xilema (Hemiptera: Cicadellidae, Cicadellinae. No presente estudo objetivou-se avaliar a flutuação populacional de espécies de cigarrinhas nas diferentes variedades de laranja doce [Citrus sinensis (L. Osbeck], Natal, Pêra, Valência e Folha Murcha, em um pomar comercial localizado na região Noroeste do Paraná, no período de janeiro de 2000 a dezembro de 2002. Amostragens quinzenais foram realizadas com o uso de armadilhas adesivas amarelas, num total de 24 armadilhas em cada avaliação. Após a identificação das espécies observou-se, que as mais representativas foram Dilobopterus costalimai da tribo Cicadellini e Acrogonia citrina da tribo Proconiini, sendo que a variedade de laranja Pêra apresentou o maior número de espécies vetoras durante os anos avaliados.

  4. Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry

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    Fernanda Januzzi Mendes-da-Glória

    2000-09-01

    Full Text Available Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck and Rangpur lime (C. limonia L. Osbeck. The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36, with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.Híbridos somáticos de laranja doce (Citrus sinensis L. Osbeck e limão Cravo (C. limonia L. Osbeck foram regenerados após a fusão (polietileno glicol e cultura de protoplastos. Os híbridos somáticos foram confirmados pela análise da morfologia das folhas, determinação do número de cromossomos e marcadores moleculares (RAPD. Todas as plantas analisadas revelaram-se tetraplóides (2n = 4x = 36, possuíam folhas de morfologia intermediária e uma combinação do padrão de bandas de RAPD de ambos os parentais. Esta combinação pode se tornar útil como porta-enxerto para a Região Sudeste da indústria citrícola brasileira. Este híbrido somático potencialmente combinará as características de tolerância à seca e o vigor do limão Cravo com a tolerância ao declínio da laranja Caipira.

  5. EFEITOS DE STIMULATE E DE MICRO-CITROS NO DESENVOLVIMENTO VEGETATIVO E NA PRODUTIVIDADE DA LARANJEIRA `PÊRA' (Citrus sinensis L. Osbeck

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    Castro P.R.C.

    1998-01-01

    Full Text Available Estudou-se o efeito de aplicações do estimulante vegetal Stimulate e do fertilizante foliar Micro-Citrus no número de ramos, comprimento dos ramos, número e caracterização de frutos na colheita da laranjeira `Pêra' (Citrus sinensis L. Osbeck sobre limoeiro `Cravo'. O experimento foi realizado em condições de campo, num Latossolo Vermelho-Escuro Álico, em Holambra (S.P., sendo que em 13/02, 22/04 e 17/06/96 foram efetuadas pulverizações com Stimulate (1 L.ha-1, 2L. ha-1 e 4L. ha-1, Stimulate 2L. ha-1 + Micro-Citros e Micro-Citros, além do controle. Realizaram-se seis tratamentos distribuídos em dez árvores inteiramente casualizadas em um pomar uniforme com seis anos de idade. Foram demarcadas quatro ramificações em diagonal, nas quais efetuaram-se determinações biométricas em 22/04 e 23/09/96. Observou-se que Stimulate (1 L. ha-1 aumentou o número de ramos 69 dias após a primeira aplicação, além de incrementar o peso médio dos frutos por árvore, em relação ao controle, na colheita.

  6. Influência de substratos na formação dos porta-enxertos: limoeiro-Cravo (Citrus limonia Osbeck e tangerineira-Cleópatra (Citrus reshni Hort. Ex Tanaka em ambiente protegido

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    JABUR MARCOS ANTÔNIO

    2002-01-01

    Full Text Available Visando a estudar o efeito do substrato na produção de porta-enxertos de limoeiro-Cravo e tangerineira-Cleópatra em tubetes sob ambiente protegido, conduziu-se este trabalho na Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal-UNESP. O estudo foi realizado em ambiente coberto com sombrite preto sem proteção lateral, adotando-se delineamento inteiramente casualizado, em esquema fatorial 5x2, 4 repetições e 20 sementes por parcela. Utilizaram-se os substratos húmus de minhoca oriundo de esterco de curral (H% e vermiculita média (V%, sendo S1=H0 V100; S2=H25 V75; S3=H50 V50; S4=H75 V25 e S5=H100 V0. Avaliaram-se o número de plântulas emergidas, altura das plantas, diâmetro do caule, massas fresca e seca de raiz e parte aérea. Observaram-se resultados estatisticamente significativos para diâmetro do caule, aos 104 e 118 dias após a semeadura (DAS entre espécies, com superioridade de Cravo em relação a Cleópatra, porém não aos 132 DAS. Não foi constatada diferença significativa entre os substratos. Quanto à massa fresca de parte aérea, nenhuma diferença estatística ocorreu entre as espécies; porém, quanto aos substratos, S3 mostrou-se superior a S1, não diferindo, entretanto, dos demais. Embora a análise estatística não tenha mostrado diferenças significativas entre os tratamentos para os outros parâmetros analisados, S3 destacou-se entre os substratos testados.

  7. Ectopic expression of MdSPDS1 in sweet orange (Citrus sinensis Osbeck reduces canker susceptibility: involvement of H2O2 production and transcriptional alteration

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    Wang Yin

    2011-03-01

    Full Text Available Abstract Background Enormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene. Results In the present work, an apple spermidine synthase gene (MdSPDS1 was introduced into sweet orange (Citrus sinensis Osbeck 'Anliucheng' via Agrobacterium-mediated transformation of embryogenic calluses. Two transgenic lines (TG4 and TG9 varied in the transgene expression and cellular endogenous polyamine contents. Pinprick inoculation demonstrated that the transgenic lines were less susceptible to Xanthomonas axonopodis pv. citri (Xac, the causal agent of citrus canker, than the wild type plants (WT. In addition, our data showed that upon Xac attack TG9 had significantly higher free spermine (Spm and polyamine oxidase (PAO activity when compared with the WT, concurrent with an apparent hypersensitive response and the accumulation of more H2O2. Pretreatment of TG9 leaves with guazatine acetate, an inhibitor of PAO, repressed PAO activity and reduced H2O2 accumulation, leading to more conspicuous disease symptoms than the controls when both were challenged with Xac. Moreover, mRNA levels of most of the defense-related genes involved in synthesis of pathogenesis-related protein and jasmonic acid were upregulated in TG9 than in the WT regardless of Xac infection. Conclusion Our results demonstrated that overexpression of the MdSPDS1 gene prominently lowered the sensitivity of the transgenic plants to canker. This may be, at least partially, correlated with the generation of more H2O2 due to increased production of polyamines and enhanced PAO-mediated catabolism, triggering hypersensitive response or activation of defense-related genes.

  8. Caracterización mediante microsatélites de 34 accesiones de naranja Citrus sinensis L. Osbeck del Banco de Germoplasma de Corpoica-Palmira

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    Morillo Coronado Yacenia

    2010-03-01

    Full Text Available Se utilizaron 19 marcadores microsatélites para caracterizar 34 accesiones de naranja Citrus sinensis L. Osbeck del Banco de Germoplasma de la Corporación Colombiana de Investigación Agropecuaria (Corpoica-Palmira. Seis marcadores fueron polimórficos, los de mayor heterocigosidad fueron CCSM- 19 (0.4097 y CCSM-3 (0.3254. Se diferenciaron 33 accesiones y al 85% de similitud, con el índice de Nei-Li y el método de agrupamiento UPGMA, se conformaron cuatro grupos. En el primero se situaron dos accesiones blancas (Lerma y Valencia Olinda 2, en el segundo la mayoría de las blancas (Indian River, Valencia Olinda, Wialua, Lima Dulce, Pera del Río, St. Michael, Enterprise, Cafetera No. 1 junto con García Valencia y Navel (Lane Late, Valle Washington, New Hall y dos sanguinas (Rudy Blood, Moro Blood, en el tercero accesiones blancas (Joppa, Salerma, Cuban Queen, Rico, Galicia, Star Calyx, Valencia Cutter, Valencia Frost, Valencia Campbell y Valencia Variegado y una sola accesión sanguina (Morocco Blood. En el cuarto grupo se situaron dos accesiones blancas, (Jaffa y Valencia 1-D-E. Finalmente, la variedad Sanguinella no hizo parte de grupos. Con los marcadores microsatélites no se encontró relación entre los grupos genéticos, formación y las características morfológicas del grupo.

  9. INFLUENCIA DE UNA COBERTURA DE Neonotonia wightii EN LOS CAMBIOS FLORÍSTICOS DE UNA PLANTACIÓN DE NARANJA VALENCIA LATE (Citrus sinensis L. Osbeck

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    I. R. Gutiérrez

    2002-01-01

    Full Text Available La necesidad de disminuir el consumo de agrotóxicos para el control de malezas en el cultivo de los cítricos, condiciona la búsqueda de alternativas viables en la esfera agroecológica, razones por las que en la Cooperativa de Producción Agropecuaria ¿José Martí¿ en Ceballos, provincia de Ciego de Avila, se evaluaron los efectos que una cobertura viva de la leguminosa Neonotonia wightii produjo en la composición florística de las ¿calles¿ de una plantación de naranja Valencia Late (Citrus sinensis L. Osbeck, usando como unidad experimental ¿la calle¿ de 400 m2, diez de las cuales contaban con cobertura mejorada por N.wightii y otras diez con pasto natural, que fueron utilizadas para la evaluación correspondiente a los indicadores de producción de los naranjos en el transcurso de dos años de establecimiento de la cobertura, obteniéndose la reducción del 68.4 % de las especies de la clase Magnoliatae inicialmente encontradas, así como del 94 % de las poáceas evaluadas en las áreas, además de la elevación de los rendimientos del cultivo principal.

  10. Caracterización mediante microsatélites de 34 accesiones de naranja Citrus sinensis L. Osbeck del Banco de Germoplasma de Corpoica-Palmira

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    Yacenia Morillo Coronado

    2010-01-01

    Full Text Available Se utilizaron 19 marcadores microsatélites para caracterizar 34 accesiones de naranja Citrus sinensis L. Osbeck del Banco de Germoplasma de la Corporación Colombiana de Investigación Agropecuaria (Corpoica-Palmira. Seis marcadores fueron polimórficos, los de mayor heterocigosidad fueron CCSM- 19 (0.4097 y CCSM-3 (0.3254. Se diferenciaron 33 accesiones y al 85% de similitud, con el índice de Nei-Li y el método de agrupamiento UPGMA, se conformaron cuatro grupos. En el primero se situaron dos accesiones blancas (Lerma y Valencia Olinda 2, en el segundo la mayoría de las blancas (Indian River, Valencia Olinda, Wialua, Lima Dulce, Pera del Río, St. Michael, Enterprise, Cafetera No. 1 junto con García Valencia y Navel (Lane Late, Valle Washington, New Hall y dos sanguinas (Rudy Blood, Moro Blood, en el tercero accesiones blancas (Joppa, Salerma, Cuban Queen, Rico, Galicia, Star Calyx, Valencia Cutter, Valencia Frost, Valencia Campbell y Valencia Variegado y una sola accesión sanguina (Morocco Blood. En el cuarto grupo se situaron dos accesiones blancas, (Jaffa y Valencia 1-D-E. Finalmente, la variedad Sanguinella no hizo parte de grupos. Con los marcadores microsatélites no se encontró relación entre los grupos genéticos, formación y las características morfológicas del grupo.

  11. The complete chloroplast genome sequence of Citrus sinensis (L. Osbeck var 'Ridge Pineapple': organization and phylogenetic relationships to other angiosperms

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    Jansen Robert K

    2006-09-01

    Full Text Available Abstract Background The production of Citrus, the largest fruit crop of international economic value, has recently been imperiled due to the introduction of the bacterial disease Citrus canker. No significant improvements have been made to combat this disease by plant breeding and nuclear transgenic approaches. Chloroplast genetic engineering has a number of advantages over nuclear transformation; it not only increases transgene expression but also facilitates transgene containment, which is one of the major impediments for development of transgenic trees. We have sequenced the Citrus chloroplast genome to facilitate genetic improvement of this crop and to assess phylogenetic relationships among major lineages of angiosperms. Results The complete chloroplast genome sequence of Citrus sinensis is 160,129 bp in length, and contains 133 genes (89 protein-coding, 4 rRNAs and 30 distinct tRNAs. Genome organization is very similar to the inferred ancestral angiosperm chloroplast genome. However, in Citrus the infA gene is absent. The inverted repeat region has expanded to duplicate rps19 and the first 84 amino acids of rpl22. The rpl22 gene in the IRb region has a nonsense mutation resulting in 9 stop codons. This was confirmed by PCR amplification and sequencing using primers that flank the IR/LSC boundaries. Repeat analysis identified 29 direct and inverted repeats 30 bp or longer with a sequence identity ≥ 90%. Comparison of protein-coding sequences with expressed sequence tags revealed six putative RNA edits, five of which resulted in non-synonymous modifications in petL, psbH, ycf2 and ndhA. Phylogenetic analyses using maximum parsimony (MP and maximum likelihood (ML methods of a dataset composed of 61 protein-coding genes for 30 taxa provide strong support for the monophyly of several major clades of angiosperms, including monocots, eudicots, rosids and asterids. The MP and ML trees are incongruent in three areas: the position of Amborella and

  12. Rare earth element transfer from soil to navel orange pulp (Citrus sinensis Osbeck cv. Newhall and the effects on internal fruit quality.

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    Jinjin Cheng

    Full Text Available The effects of soil rare earth element (REE on navel orange quality and safety in rare earth ore areas have gained great attention. This study investigated the transfer characteristics of REE from soil to navel orange pulp (Citrus sinensis Osbeck cv. Newhall and examined the effects of soil REE on internal fruit quality in Xinfeng County, Jiangxi province, China. Path analysis showed that soil REE, pH, cation exchange capacity (CEC, and Fe oxide (Feox significantly affected pulp REE concentrations. A Freundlich-type prediction model for pulp REE was established: log[REEpulp] = -1.036 + 0.272 log[REEsoil] - 0.056 pH - 0.360 log[CEC] + 0.370 log[Feox] (n = 114, R2 = 0.60. From the prediction model, it was inferred that even when soil REE and Feox were as high as 1038 mg kg-1 and 96.4 g kg-1, respectively, and pH and CEC were as low as 3.75 and 5.08 cmol kg-1, respectively, pulp REE concentrations were much lower than the food limit standard. Additionally, soil REE levels were significantly correlated with selected fruit quality indicators, including titratable acidity (r = 0.52, P < 0.01, total soluble solids (r = 0.48, P < 0.01 and vitamin C (r = 0.56, P < 0.01. Generally, under routine methods of water and fertilization management, the cultivation of navel oranges in rare earth ore areas of south China with soil REE ranging from 38.6 to 546 mg kg-1 had improved in internal fruit quality.

  13. Visitantes florais e produção de frutos em cultura de laranja (Citrus sinensis L. Osbeck - DOI: 10.4025/actascianimsci.v28i4.612 Floral visitors and fruit production on sweet orange crop (Citrus sinensis L. Osbeck - DOI: 10.4025/actascianimsci.v28i4.612

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    Darclet Terezinha Malerbo Souza

    2006-02-01

    Full Text Available O presente experimento foi realizado em florada de laranja (Citrus sinensis L. Osbeck, variedade Pera-Rio, com os objetivos de estudar os insetos visitantes nas flores d e laranjeira, o seu comportamento nas flores, o tipo de coleta efetuada e o efeito dessas visitas na produção de frutos, em quantidade e qualidade. Os dados de freqüência foram obtidos por contagem nos primeiros 10 minutos de cada horário, das 8h às 18h, em três dias distintos, percorrendo-se as linhas da cultura. O comportamento forrageiro de cada espécie de inseto foi avaliado através de observações visuais, no decorrer do dia, no período experimental. Os insetos observados foram abelhas africanizadas Apis mellifera, Trigona spinipes e Tetragonisca angustula. As abelhas A. mellifera foram os visitantes florais mais freqüentes e preferiram coletar néctar comparado ao pólen. Os botões florais descobertos produziram mais frutos que os botões florais cobertos. Os frutos decorrentes do tratamento coberto foram menores, mais ácidos e com menor quantidade de vitamina C que os frutos do tratamento descoberto.The present experiment was carried out in flowerage of sweet orange (Citrus sinensis L. Osbeck, Pera-rio variety, to study the insects involv ed in pollination, their behaviour in the flower (nectar or pollen collection and the effect of the pollination on fruit production (quantity and quality. More frequent insects were recorded daily (counted during ten minutes, every hour from 8:00 a.m. to 6:00 p.m., with three replications. The forage behaviour and nectar and/or pollen collect was also observed. The insect visitors on flowers were Africanized honey bee Apis mellifera, followed by stingless bees Trigona spinipes and Tetragonisca angustula. A. mellifera were the most frequent visitors and preferred to collect nectar than pollen. The uncovered flowers -buds produced more fruits than the covered ones. Another observation was that fruits derived from covered

  14. β-cyclodextrin inclusion complexes containing Citrus sinensis (L.) Osbeck essential oil: An alternative to control Aedes aegypti larvae

    Energy Technology Data Exchange (ETDEWEB)

    Galvão, J.G., E-mail: julianaggalvao@gmail.com [Pharmacy Department, Federal University of Sergipe, 49100-000 São Cristóvão, SE (Brazil); Silva, V.F.; Ferreira, S.G. [Pharmacy Department, Federal University of Sergipe, 49100-000 São Cristóvão, SE (Brazil); França, F.R.M. [Chemical Engineering Department, Federal University of Sergipe, 49100-000 São Cristóvão, SE (Brazil); Santos, D.A.; Freitas, L.S.; Alves, P.B. [Chemistry Department, Federal University of Sergipe, 49100-000 São Cristóvão, SE (Brazil); Araújo, A.A.S.; Cavalcanti, S.C.H.; Nunes, R.S. [Pharmacy Department, Federal University of Sergipe, 49100-000 São Cristóvão, SE (Brazil)

    2015-05-20

    Highlights: • Thermal analysis was useful to determine the formation of inclusion complexes by paste and co-precipitation methods. • HS/GC-FID quantitative analysis revealed that the best method of obtaining a CSEO/β-CD complex was the PWE, with the largest inclusion content [78.5%]. • The inclusion complex revealed LC{sub 50} of 23.01 ppm, close to CSEO LC{sub 50} 21.5 ppm. - Abstract: The development of β-cyclodextrin (β-CD) complexes is an interesting way for increasing the aqueous solubility of essential oils. The aim of this study was to prepare inclusion complexes of Citrus sinensis essential oil (CSEO) with β-CD using paste complexation (with and without co-solvent) and co-precipitation methods. Additionally, the physicochemical properties of the inclusion complexes using thermal analysis, X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy were evaluated. Furthermore, CSEO content (%) and solubility of complexes were measured. The biological activity against the Aedes aegypti Linn. larvae was further evaluated. For comparison purposes, a physical mixture between β-CD and CSEO was prepared and evaluated. Thermal analysis clearly indicated the formation of complexes by paste and co-precipitation methods. The headspace/gas chromatography quantitative analysis showed inclusions contents higher than 50%. On the other hand, the product revealed LC{sub 50} of 23.01 ppm, close to CSEO LC{sub 50} 21.5 ppm.

  15. β-cyclodextrin inclusion complexes containing Citrus sinensis (L.) Osbeck essential oil: An alternative to control Aedes aegypti larvae

    International Nuclear Information System (INIS)

    Highlights: • Thermal analysis was useful to determine the formation of inclusion complexes by paste and co-precipitation methods. • HS/GC-FID quantitative analysis revealed that the best method of obtaining a CSEO/β-CD complex was the PWE, with the largest inclusion content [78.5%]. • The inclusion complex revealed LC50 of 23.01 ppm, close to CSEO LC50 21.5 ppm. - Abstract: The development of β-cyclodextrin (β-CD) complexes is an interesting way for increasing the aqueous solubility of essential oils. The aim of this study was to prepare inclusion complexes of Citrus sinensis essential oil (CSEO) with β-CD using paste complexation (with and without co-solvent) and co-precipitation methods. Additionally, the physicochemical properties of the inclusion complexes using thermal analysis, X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy were evaluated. Furthermore, CSEO content (%) and solubility of complexes were measured. The biological activity against the Aedes aegypti Linn. larvae was further evaluated. For comparison purposes, a physical mixture between β-CD and CSEO was prepared and evaluated. Thermal analysis clearly indicated the formation of complexes by paste and co-precipitation methods. The headspace/gas chromatography quantitative analysis showed inclusions contents higher than 50%. On the other hand, the product revealed LC50 of 23.01 ppm, close to CSEO LC50 21.5 ppm

  16. Growth of citrus rootstocks under aluminium stress in hydroponics

    Directory of Open Access Journals (Sweden)

    Pereira Walter Esfrain

    2003-01-01

    Full Text Available Plants present different degrees of adaptation to aluminium (Al concentrations in the soil, and the understanding of this characteristic can lead to a viable option for the utilization of acid soils. The objective of this experiment was to evaluate the effects of five Al concentrations (0, 50, 100, 200, and 400 mumol L-1 on the growth of 'Rangpur' lime (Citrus limonia Osbeck and 'Volkamer' lemon (Citrus volkameriana Hort. ex Tan., and tangerine rootstocks 'Cleópatra' (Citrus reshni Hort. ex Tan and 'Sunki' (Citrus sunki Hort. ex Tan., in hydroponic culture. The treatments were arranged in a randomized block design, with four replications. For all rootstocks, the relative growth rate in terms of plant total fresh matter increased under low and, decreased under large Al concentrations. Growth of the shoot, leaf area ratio and leaf weight ratio decreased for all rootstocks in the presence of Al. The 'Rangpur' lime had a decrease of the root system growth, starting from 23 mumol L-1 of Al. For the remaining rootstocks, this growth reached maximum values at 91 to 117 mumol L-1 of Al, respectively. Considering all the evaluated characteristics of plant growth, the 'Rangpur' lime was the most susceptible to Al.

  17. Mites (Arachnida, Acari on Citrus sinensis L. Osbeck orange trees in the state of Amazonas, Northern Brazil Ácarofauna de Citrus sinensis L. Osbeck no estado do Amazonas, Brasil

    Directory of Open Access Journals (Sweden)

    Teiamar da Encarnação Bobot

    2011-01-01

    Full Text Available Despite the importance of citriculture in Brazil, very little is known about mite populations in citrus crops in the Northern Region. In the municipality of Manaus, 12 sprayed sweet orange orchards were surveyed every two weeks during seven months to record mite species amount, and to describe the abundance and distribution of the most important species. The size and age of the orchards varied from 3,360 to 88,080 m² and seven to 25 years, respectively. In the fourteen sampling period, leaves, twigs and fruits were collected from 12 trees, one per orchard. In total, 3,360 leaves, 672 twigs and 1,344 fruits were sampled from 168 trees. Mites were manually extracted from the fruits, and by the washing method on leaves and twigs. We identified pests with the potential to cause economic loss. Fourteen species of phytophagous and mycophagous mites from Eriophyidae, Tarsonemidae, Tenuipalpidae, and Tetranychidae were recorded. Brevipalpus phoenicis (Geijskes 1939 and Phyllocoptruta oleivora (Ashm., 1879, the two commonest phytophagous mites in other Brazilian regions were dominant, showing that local orchards are susceptible to their infestation. Eleven predatory mites were recorded, comprising 10% of the mite population, belonging to Phytoseiidae and Ascidae. Phytoseiidae was the richest family, with ten species. The results are discussed in relation to the temporal variation aspects and habitat use of the most important species. Long-term research encompassing chemical applications followed by evaluations of the mite community are necessary for a better management of the orchards, taking into consideration the seasonal phenology of key pests.Apesar da importância da citricultura no Brasil, pouco se conhece sobre as populações de ácaros em plantações de citros no norte do país. No município de Manaus, 12 pomares de laranja doce pulverizados foram avaliados a cada duas semanas, durante sete meses, para o registro de ácaros plantícolas e

  18. Desenvolvimento vegetativo e custo de produção de porta-enxertos de citros em recipientes para fins de subenxertia Vegetative development and production cost of citrus rootstocks in containers for inarching

    Directory of Open Access Journals (Sweden)

    Eduardo Augusto Girardi

    2007-05-01

    Full Text Available O objetivo deste trabalho foi avaliar o desenvolvimento vegetativo e estimar o custo de produção de 11 porta-enxertos de citros para fins de subenxertia, em diferentes recipientes. Avaliaram-se limão 'Cravo' clone Limeira (Citrus limonia Osbeck; citrumelo 'Swingle' (Poncirus trifoliata (L. Raf. x Citrus paradisi Macf.; tangerina 'Cleópatra' (Citrus reshni Hort. ex Tanaka; tangerina 'Sunki' (Citrus sunki Hort. ex Tanaka; limão 'Volkameriano' clone Catânia 2 (Citrus volkameriana Pasquale; laranja 'Caipira' clone DAC (Citrus sinensis L. Osbeck; limão 'Rugoso da África' clone Mazoe (Citrus jambhiri Lush.; Poncirus trifoliata 'Davis A'; tangerina 'Sun Shu Sha Kat' (Citrus sunki Hort. ex Tanaka; tangerina 'Sunki' clone 2506 ou Fruto Grande (Citrus sunki Hort. ex Tanaka e Poncirus trifoliata 'Barnes'. Foram utilizados tubetes de 290 mL, sacolas de 1,7 L, e porta-enxertos transplantados de tubetes de 75 mL para sacolas de polietileno de 1,7 e 4,5 L. Porta-enxertos produzidos diretamente em sacolas de 1,7 L atingem ponto ideal de subenxertia em menor tempo, de 100 a 150 dias após a semeadura, e permitem a obtenção de plantas maiores e com sistema radicular adequado, porém com custo de produção superior ao sistema de produção em tubetes de 290 mL.The vegetative development and the estimation of the production cost of eleven citrus rootstocks for inarching were evaluated in different containers. 'Rangpur' lime cultivar Limeira (Citrus limonia Osbeck; 'Swingle' citrumelo (Poncirus trifoliata (L. Raf. x Citrus paradisi Macf.; 'Cleópatra' mandarin (Citrus reshni Hort. ex Tanaka; 'Sunki' mandarin (Citrus sunki Hort. ex Tanaka; 'Volkamer' lemon cultivar Catânia 2 (Citrus volkameriana Pasquale; 'Caipira' sweet orange cultivar DAC (Citrus sinensis L. Osbeck; 'Rugoso da África' rough lemon cultivar Mazoe (Citrus jambhiri Lush.; Poncirus trifoliata cultivar Davis A; 'Sun Shu Sha Kat' mandarin (Citrus sunki Hort. ex Tanaka; 'Sunki' mandarin

  19. 两类柑桔杂种砧木对脐橙(Citrus sinensis Osbeck)幼树生长和叶片糖营养含量年变化的影响%Effects of Two Kinds of Rootstocks on the Growth of Navel Orange(Citrus sinensis Osbeck)Young Tree and the Annual Changes in the Contents of Carbohydrates in Leaves

    Institute of Scientific and Technical Information of China (English)

    周开兵; 郭文武; 夏仁学; 王贵元; 沈婷

    2004-01-01

    以体细胞杂种红桔+枳和红桔+粗柠檬、有性杂种Troyer枳橙和Swingle枳柚作砧木的耐湿脐橙(Citrus sinensis Osbeck)2年生嫁接苗为试材,通过盆栽试验研究了砧木对苗木生长和叶片糖营养含量年变化动态的影响.结果表明:红桔+枳的生长势和花量明显地强于和大于其它砧木,红桔+粗柠檬的生长和花量表现介于2种有性杂种之间.叶绿素a/b和类胡萝卜素含量在不同砧木间无显著差异,而不同砧木显著或极显著地影响叶绿素含量各指标.砧木影响叶片可溶性糖和淀粉含量年变化动态的基本特征.在休眠期,红桔+枳的可溶性糖和淀粉含量都高于2种有性杂种砧木,而红桔+粗柠檬则反之;在生长期,红桔+粗柠檬的可溶性糖和淀粉含量表现出高于有性杂种砧木的趋势,而红桔+枳则介于2种有性杂种砧木之间.同时还重点讨论了2种体细胞杂种砧木的利用价值.

  20. 咪鲜胺壳寡糖复合涂膜对脐橙果实采后品质的影响%Effect of Oligochitosan and Prochloraz Complex Coating on the Postharvest Quality of Citrus Sinensis Osbeck

    Institute of Scientific and Technical Information of China (English)

    聂青玉

    2012-01-01

    以脐橙为试材,果实采后以1%的壳寡糖为涂膜剂,分别与质量分数为0.01%,0.05%,0.10%浓度的咪鲜胺(有效成份450 g/L水乳剂)组合,浸泡处理后置于18~25℃,75%~85%RH环境中贮藏,比较与不涂膜处理及仅用1%的壳寡糖处理对脐橙贮藏品质、采后生理的影响.结果表明:不同浓度的咪鲜胺壳寡糖复合涂膜均能降低果实的失重率、腐烂率;延缓果实可溶性固形物、可滴定酸、还原性抗坏血酸含量的下降;抑制呼吸强度、丙二醛含量、相对电导率的上升;有效保持贮藏脐橙果实的感官品质、风味品质、营养品质,延长果实的贮藏期.其中,0.05%咪鲜胺与1%壳寡糖复合涂膜处理果实的各项品质、生理指标优于其它处理组,为有效延长脐橙果实贮藏时间的最经济、安全组合浓度.%Postharvest Citrus sinensis Osbeck has been stored after being soaked in 18 - 25 ℃ , 75% -85% RH environment,by the treatment of 1. 0% of Oligochitosan Combined with 0. 01 %, 0. 05% and 0. 1 % of Prochloraz (active ingredient 450 g/L of water emulsion) respectively. The effects of 1 % Oligochitosan treatment and uncoating treatment on the postharvest physiology and storage quality of Citrus sinensis Osbeck have been compared. The result illustrates that all kinds of Oligochitosan and Prochloraz Complex coating could reduce rate of weight loss and decay, delay the decline of soluble solids, titratable acidity and the content of ascorbic acid in fruits, inhibit the increase of the respiration rate, MDA content and relative electrical conductivity, maintain effectively the sensory, flavor, nutritional quality of Citrus sinensis Osbeck stored, and extend the storage period of Citrus sinensis Osbeck. Furthermore, the most economical and secure combination is 0.05% of Prochloraz (active ingredient 450 g/L of water emulsion) and 1% of Oligochitosan, which can make all kinds of the quality, physiological indexes

  1. Efeito do tratamento agudo do óleo essencial de Citrus sinensis (L Osbeck na aquisição da memória espacial de ratos avaliada no labirinto aquático de Morris

    Directory of Open Access Journals (Sweden)

    Ciro Gonçalves Sá

    2012-04-01

    Full Text Available A Doença de Alzheimer está relacionada a prejuízos na aquisição e retenção da memória, processos que podem ser estudados no laboratório por meio de modelos animais, entre eles o labirinto aquático de Morris, que avalia a memória espacial em ratos. Estudos sobre as propriedades biológicas do gênero Citrus destacam atividades importantes como antioxidantes e anticolinesterásica. O objetivo desse trabalho é avaliar os efeitos do tratamento agudo com o óleo essencial de folhas (OEF de Citrus sinensis (L. Osbeck no processo de aquisição da memória espacial em ratos Wistar, utilizando o paradigma do labirinto aquático de Morris. O óleo essencial de Citrus sinensis possui em sua composição principalmente compostos da classe dos monoterpenos, como o limoneno (20,14%, citronelol (30,42% e o geranial (31,42%. Os animais foram tratados previamente com doses do óleo essencial (OE de C. sinensis (L. Osbeck de 50, 100 e 200 mg/ kg e realizados testes de campo aberto e do labirinto aquático de Morris. A aquisição da memória espacial é avaliada pelo tempo que o animal leva para localizar a plataforma depois de ter sido treinado. Nos resultados do campo aberto foi demonstrado que os animais não apresentam estímulo motor quando tratados com o óleo essencial de Citrus sinensis e os resultados do labirinto aquático foram significativamente menores na latência para encontrarem a plataforma submersa do que o grupo controle negativo [p<0,01] indicando uma capacidade de memória maior nos animais tratados, mas que devem ser reforçados por outros testes de memória preconizado na literatura.

  2. Use of the heat dissipation method for sap flow measurement in citrus nursery trees1 Uso do método de dissipação de calor para a medição do fluxo de seiva em mudas cítricas

    Directory of Open Access Journals (Sweden)

    Eduardo Augusto Girardi

    2010-12-01

    Full Text Available Sap flow could be used as physiological parameter to assist irrigation of screen house citrus nursery trees by continuous water consumption estimation. Herein we report a first set of results indicating the potential use of the heat dissipation method for sap flow measurement in containerized citrus nursery trees. 'Valencia' sweet orange [Citrus sinensis (L. Osbeck] budded on 'Rangpur' lime (Citrus limonia Osbeck was evaluated for 30 days during summer. Heat dissipation probes and thermocouple sensors were constructed with low-cost and easily available materials in order to improve accessibility of the method. Sap flow showed high correlation to air temperature inside the screen house. However, errors due to natural thermal gradient and plant tissue injuries affected measurement precision. Transpiration estimated by sap flow measurement was four times higher than gravimetric measurement. Improved micro-probes, adequate method calibration, and non-toxic insulating materials should be further investigated.O fluxo de seiva poderia ser utilizado como parâmetro fisiológico para fomentar a irrigação de mudas cítricas em cultivo protegido pela estimação do consumo contínuo de água. Neste trabalho, reportam-se os primeiros resultados, indicando o uso potencial e as limitações iniciais do método de dissipação de calor para medição do fluxo de seiva em mudas cítricas em recipientes. Mudas de laranjeira-doce 'Valência' [Citrus sinensis (L. Osbeck] enxertadas sobre limoeiro 'Cravo' (Citrus limonia Osbeck foram avaliadas por 30 dias, durante o verão. Sondas de dissipação de calor e sensores do tipo termopar foram construídos a partir de materiais prontamente disponíveis e de baixo custo para favorecer o acesso ao método por viveiristas. O fluxo de seiva apresentou alta correlação com a temperatura do ar dentro da estufa telada. Contudo, erros inerentes ao gradiente térmico natural e a injúrias nos tecidos do caule afetaram a

  3. 甜橙挥发油成分及药理研究进展%Sweet orange (Citrus sinensis L.(Osbeck)) of volatile oil constituents and pharmacological research

    Institute of Scientific and Technical Information of China (English)

    陈莹

    2011-01-01

    甜橙Citrus sinensis L.(Osbeck)为芸香科(Rutaceae)柑橘亚科柑橘属(Citrus)植物,属于乔木.西汉时(公元前117年)已有"黄柑、橙、楱"等关于甜橙的记载.甜橙又名广柑、黄果、橙子、新会橙,广橘、雪柑、印子柑[1],原产于我国南方及亚洲的中南半岛,在15世纪初期从中国传入欧洲,在15世纪末传入美洲.在我国,甜橙主产于四川、广东、台湾、广西、福建、湖南、江西、湖北等省.在全球各种柑橘的年产量中,甜橙所占的比例最大,其年产量约占橘类总产量的2/3[2].而且,甜橙品种在柑橘类中最为丰富,全世界品种达400个以上.

  4. Fita plástica e fita degradável na enxertia de citros Plastic and degradable tape on citrus budding

    Directory of Open Access Journals (Sweden)

    Roberto Pedroso de Oliveira

    2004-12-01

    Full Text Available Este trabalho foi realizado com o objetivo de avaliar o efeito de fitas plástica e degradável no pegamento e no desenvolvimento de enxertos de citros, em viveiro telado. Borbulhas certificadas de 6-8 mm das cultivares de laranjeira-de-umbigo 'Navelina' (Citrus sinensis (L. Osbeck, tangerineira 'Marisol' (C. reticulata Blanco e do híbrido 'Nova' [C. clementina x (C. paradisi x C. tangerina ] foram enxertadas em limoeiro 'Cravo' (C. limonia Osbeck, com 10 meses de idade, em fevereiro de 2003. A fixação das borbulhas nos porta-enxertos foi feita com fitas de dois tipos: fita plástica de polietileno transparente e fita degradável de parafilme. Os tratamentos foram dispostos em esquema fatorial (3 cultivares x 2 tipos de fita, em delineamento inteiramente ao acaso, com quatro repetições, sendo as unidades experimentais constituídas por dez plantas. A média geral de pegamento dos enxertos foi elevada (99,6%, em razão das condições propícias à enxertia fornecidas no viveiro telado. Não houve efeito do tipo de fita no pegamento dos enxertos nem das cultivares no pegamento e desenvolvimento dos enxertos. No entanto, o desenvolvimento dos enxertos foi sensivelmente maior quando a enxertia foi realizada com fita degradável em relação à fita plástica.The aim of this work was to evaluate the effect of the plastic and degradable tape on budding efficiency and scion development of citrus in nursery under screenhouse. Certificated 6-8 mm buds of the cultivars navel orange 'Navelina' (Citrus sinensis (L. Osbeck, mandarin 'Marisol' (C. reticulata Blanco and the hybrid 'Nova' [C. clementina x (C. paradisi x C. tangerina ] were budded on 10 months rangpur lime (C. limonia Osbeck, in February 2003. The buds were attached to the rootstocks using either one of two types of tape: plastic polyethylene transparent tape and parafilm degradable tape. The experimental design was a factorial entirely randomized with 3 cultivars, 2 tape types and four

  5. 脐橙凝胶型果派产品配方优化及工艺研究%Research on Formulation and Process Optimization of Citrus sinensis Osbeck gel Style Candy

    Institute of Scientific and Technical Information of China (English)

    罗秋潜; 刘幸科; 杨禹晗; 欧阳健; 朱建华; 钟瑞敏; 邹秀容

    2015-01-01

    以新鲜脐橙果浆、食品亲水胶体、果葡糖浆与白砂糖为主要原料制备脐橙凝胶型果派产品。以感官品评总体评价分值为产品质量评价指标,在单因素实验结果基础上,通过正交实验获得最优配方为:47.0%(质量比)甜味剂(果葡糖浆:白砂糖为1.3∶1)、13.0%(质量比)脐橙果浆、1.7%(质量比)胶凝剂(琼脂1.3%、黄芪胶0.2%、卡拉胶0.2%)、1.1%(质量比)柠檬酸。该配方下制备的产品色泽均一,具典型脐橙香味,酸甜味适中,并具良好的咀嚼性和弹性等质构特性。%In this paper the Citrus sinensis Osbeck gel style candy was prepared mainly with fresh orange pulp , food hydrocolloids, high fructose syrup and sugar. Based on single factor and orthogonal experiments, combined with overall sensory evaluation score as quality indicators the optimal formula was obtained as follows:47.0%(w/w)sweeteners(high fruit syrup to sugar as 1.3∶1),13.0%(w/w)orange pulp, 1.7%(w/w)gelling agent(agar 1.3%,tragacanth 0.2%, carrageenan0.2%), 1.1%(w/w) citric acid. The gel style candy prepared with the optimal formula had uniform color, a typical Citrus sinensis Osbeck flavor, moderate sweet and sour taste, and with good texture such as chewing and elasticity characteristics.

  6. 紫外线照射对梁平柚果皮基因表达的影响%Effect of UV-B Radiation on Gene Expression in Peels of'Liangping'Pommelo[Citrus grandis(L.)Osbeck

    Institute of Scientific and Technical Information of China (English)

    程春振; 朱世平; 吴波; 阳佳位; 贝学军; 马岩岩; 钟广炎

    2011-01-01

    A suppression subtractive hybridization library was successfully constructed using cDNA synthesized from RNA extracted from peels of 'Liangping' pemmelo[Citrus grandis (L.) Osbeck]receiving UV-B radiation as tester and non-UVB treatment as driver.Two hundred randomly-picked positive clones were sequenced, and a total of 168 EST sequences were obtained.One hundred and fourteen ESTs, involving in 57 genes, were found to share considerable homology with known genes while the rest 54 ESTs had low or even no homology with known genes.Function analysis showed that these differentially expressed genes were related to numerical celluar events such as stress-tolerance, growth and development, cell apoptosis, cell differenciation, gene transcription and translation, signaling, energy metabolism, carbohydrate and amino acid metabolism and secondary metabolism.%采用SSH技术以紫外照射的梁平柚[Citrus grandis(L.)Osbeck]果皮作为实验方(tester),以未被照射的正常果皮作为驱动方(driver),构建了一个梁平柚果皮紫外诱导基因的正向差减文库.经菌液PCR检测后随机挑取200个阳性克隆测序,获得168条表达序列标签(ESTs).比对这168条ESTs,发现有分属于57个基因的114条ESTs与已知基因高度同源,54条ESTs同源性较低或没有同源性.功能分析发现,这些ESTs主要参与抗逆防御、生长发育、细胞凋亡、转录与翻译、细胞分化、信号传导、能量代谢、糖类及氨基酸代谢以及次生代谢等.

  7. The complete chloroplast genome sequence of Citrus sinensis (L.) Osbeck var 'Ridge Pineapple': organization and phylogenetic relationships to other angiosperms

    OpenAIRE

    Jansen Robert K; Lee Seung-Bum; Singh Nameirakpam D; Bausher Michael G; Daniell Henry

    2006-01-01

    Abstract Background The production of Citrus, the largest fruit crop of international economic value, has recently been imperiled due to the introduction of the bacterial disease Citrus canker. No significant improvements have been made to combat this disease by plant breeding and nuclear transgenic approaches. Chloroplast genetic engineering has a number of advantages over nuclear transformation; it not only increases transgene expression but also facilitates transgene containment, which is ...

  8. Carbon cost of the fungal symbiont relative to net leaf P accumulation in a split-root VA mycorrhizal symbiosis. [Poncirus trifoliata L. Raf. x Citrus sinensis L. Osbeck; Glomus intraradices Schenk and Smith

    Energy Technology Data Exchange (ETDEWEB)

    Douds, D.D. Jr.; Johnson, C.R.; Koch, K.E. (Univ. of Florida, Gainesville (USA))

    1988-02-01

    Translocation of {sup 14}C-photosynthates to mycorrhizal (++), half mycorrhizal (0+), and nonmycorrhizal (00) split-root systems was compared to P accumulation in leaves of the host plant. Carrizo citrange seedlings (Poncirus trifoliata (L.) Raf. {times} Citrus sinensis (L.) Osbeck) were inoculated with the vesicular-arbuscular mycorrhizal fungus Glomus intraradices Schenck and Smith. Plants were exposed to {sup 14}CO{sub 2} for 10 minutes and ambient air for 2 hours. Three to 4% of recently labeled photosynthate was allocated to metabolism of the mycorrhiza in each inoculated root half independent of shoot P concentration, growth response, and whether one or both root halves were colonized. Nonmycorrhizal roots respired more of the label translocated to them than did mycorrhizal roots. Label recovered in the potting medium due to exudation or transport into extraradical hyphae was 5 to 6 times greater for (++) versus (00) plants. In low nutrient media, roots of (0+) and (++) plants transported more P to leaves per root weight than roots of (00) plants. However, when C translocated to roots utilized for respiration, exudation, etc., as well as growth is considered, (00) plant roots were at least as efficient at P uptake (benefit) per C utilized (cost) as (0+) and (++) plants. Root systems of (++) plants did not supply more P to leaves than (0+) plants in higher nutrient media, yet they still allocated twice the {sup 14}C-photosynthate to the mycorrhiza as did (0+) root systems.

  9. Character impact odorants of Citrus Hallabong [(C. unshiu Marcov x C. sinensis Osbeck) x C. reticulata Blanco] cold-pressed peel oil.

    Science.gov (United States)

    Choi, Hyang-Sook

    2003-04-23

    The volatile components of Hallabong ([C. unshiu Marcov x C. sinensis Osbeck] x C. reticulata Blanco) cold-pressed peel oil were quantitatively and qualitatively determined by use of two internal standards with GC, GC-MS, and GC-olfactometry. According to instrumental analysis by GC and GC-MS, limonene (90.68%) was the most abundant compound, followed by sabinene (2.15%), myrcene (1.86%), and gamma-terpinene (0.88%). Flavor dilution (FD) factors of the volatile flavor components from Hallabong peel oil were determined by aroma extract dilution analysis. Furthermore, relative flavor activity was investigated by means of FD factor and weight percent. The highest FD factors were found for citronellal and citronellyl acetate, and delta-murollene showed a higher relative flavor activity. Results of sniff testing of the original oil and its oxygenated fraction revealed that citronellal, cis-beta-farnesene, and citronellyl acetate were regarded as the character impact odorants of Hallabong peel oil, and citronellal gave the most odor-active character of Hallabong aroma. PMID:12696958

  10. Compare the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis( L.) Osbeck and citrus grandis( L.)Osbeck%橘、橙、柚皮中橙皮苷和柚皮苷含量的测定

    Institute of Scientific and Technical Information of China (English)

    边清泉; 刘思曼; 杨振萍; 黄宝美

    2008-01-01

    橘、橙、柚皮分别为芸香科植物橘(citrus reticulata Blnco)、橙(citrussimenis(L) Osbeck)、柚(citrus grandis(L)Osbeck)及其栽培变种的干燥成熟果皮。橘皮(陈皮)为历版药典收录的中药材,而橙、柚皮资源的开发多年来被人们忽略,较多地重视其果实的利用,除极少部分作为中药材或提取果胶和香精油的原料外,在果实食用或加工后将占果总重量约40%-50%果皮渣弃去,从环境、经济和资源利用角度都不尽科学合理。

  11. Influencia de 46 porta-injertos para cítricos en la precocidad o retardo de maduración de la naranja "Frost valencia" (Citrus sinensis (L. Osbeck

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    Caselles N. Álvaro A.

    1987-03-01

    Full Text Available Of 46 graft carrier influence in citrics on ripe precocity or retardation from "Frost Valencia" Citrus sinensis (L Osbeck was studied looking for to guaranter a fruit constant supply toward market. Graft carrier influence was tested in orange blosson, fruiting ripeness season. Stock influence to induce early intermediate or later blossoms was observed, related with period and intensity so them same ocurr. Stock influence on solubles solids content, juici volume, ripeness index, ripe fruit, mantenence on tree also was found . Early of later yields are feasible to obtain using trifoliado x Ruby Or (1437 and "Garcia Valencia" stock markedly, or which highly significant ripeness gains were found with "Frost Valencia" Orange respectively.Se estudió la influencia de los portainjertos para cítricos buscando la obtención de producciones tempranas o tardías para garantizar un suministro continuo de fruta fresca al mercado. Se analizó por espacio de dos años la influencia de los porta-injertos en el período de floración, fructificación y maduración de la naranja. Se observó influencia del patrón para inducir floraciones precoces, intermedias o tardías haciendo referencia al período de intensidad con que ocurren las mismas. También se encontró influencia del patrón en contenido de sólidos solubles, volumen de jugo, acidez, índice de marez y mantenimiento de la fruta madura en el árbol. Es posible obtener producciones tempranas o tardías con la utilización de los patrones trifoliados x Ruby Or (1437 Y García Valencia, sobre las cuales se encontró adelantos altamente significativos de maduración de la naranja "Frost Valencia" respectivamente.

  12. Efecto del déficit hídrico en el crecimiento y desarrollo de frutos de naranja Valencia (Citrus sinensis Osbeck en el piedemonte del Meta, Colombia

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    Diana Lucía Garzón Correa

    2013-04-01

    Full Text Available En Colombia el área sembrada con cítricos es marginal, si se compara con el área potencial de cultivo, debido a factores como déficit de agua, nutrición y eficiencia de la formación de frutos. El objetivo del presente estudio fue evaluar el efecto del déficit hídrico mediante la aplicación controlada de láminas de riego en el cultivo de naranja Valencia (Citrus sinensis Osbeck en el piedemonte del Meta (departamento del Meta, Colombia. Los tratamientos (T de riego fueron: T1 = 100% de la evapotranspiración del cultivo (ETc; T2 = 50% ETc; T3 = testigo = 0% ETc. El riego se aplicó desde el inicio de la floración en febrero hasta la formación del fruto en abril de 2011. El diseño experimental fue bloques completamente al azar con tres repeticiones por tratamiento. Las variables evaluadas fueron: humedad del suelo (q g, estado hídrico de la planta (Ytallo, fenología, brotación, floración, formación, crecimiento y calidad del fruto. Los árboles en el T1 presentaron un mayor número y tamaño de fruto, pero no se encontraron diferencias (P > 0.05 en producción y calidad respecto al testigo; la q g y el Ytallo fueron diferentes (P < 0.05 entre tratamientos y el testigo mostró los menores valores, 13.82% y -2.5 MPa, lo cual indica un estrés moderado.

  13. Comparison of Flavonoid Compounds in the Flavedo and Juice of Two Pummelo Cultivars (Citrus grandis L. Osbeck from Different Cultivation Regions in China

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    Mingxia Zhang

    2014-10-01

    Full Text Available The objective of this study was to investigate the effect of different cultivation regions on the pattern and content of flavonoids in two pummelo cultivars (C. grandis L. Osbeck in China. Results showed that similar patterns of flavonoids were observed in the flavedo or juice of each pummelo cultivar from these cultivation regions, whereas the individual flavonoid content showed unique characteristics. Naringin, the predominant flavanone glycoside, showed the highest content in both flavedo and juice of C. grandis “Guanximiyu” from the Pinghe of Fujian (FJ cultivation region compared with the Dapu of Guangdong (GD and Nanbu of Sichuan (SC regions. However, its content in the flavedo of C. grandis “Shatianyu” from the Pingle of Guangxi (GX was significantly lower than in the GD and SC regions. Vicenin-2 appeared to be the dominant flavone C-glycoside in the flavedo of both cultivars, and the lowest content was observed in the flavedo of C. grandis “Guanximiyu” from the SC region. However, C. grandis “Shatianyu” contained the highest content of vicenin-2 in the flavedo from SC region. Similarly, the predominant flavone O-glucoside, rhoifolin, showed the highest content in C. grandis “Guanximiyu” from the GD and FJ regions, whereas C. grandis “Shatianyu” in SC region showed the highest content of rhoifolin. Cluster analysis suggested that genotype played a primary role in determining the flavonoid profiles of pummelo cultivars, whereas regional differences significantly affected the flavonoid distribution of pummelo cultivars potentially via affecting the direction of flavonoid accumulation in pummelo.

  14. Two Lycopene β-Cyclases Genes from Sweet Orange (Citrus sinensis L. Osbeck) Encode Enzymes With Different Functional Efifciency During the Conversion of Lycopene-to-Provitamin A

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jian-cheng; ZHOU Wen-jing; XU Qiang; TAO Neng-guo; YE Jun-li; GUO Fei; XU Juan; DENG Xiu-xin

    2013-01-01

    Citrus fruits are rich in carotenoids. In the carotenoid biosynthetic pathway, lycopene β-cyclase (LCYb, EC:1.14.-.-) is a key regulatory enzyme in the catalysis of lycopene to β-carotene, an important dietary precursor of vitamin A for human nutrition. Two closely related lycopeneβ-cyclase cDNAs, designated CsLCYb1 and CsLCYb2, were isolated from the pulp of orange fruits (Citrus sinensis). The expression level of CsLCYb genes is lower in the lfavedo and juice sacs of a lycopene-accumulating genotype Cara Cara than that in common genotype Washington, and this might be correlated with lycopene accumulation in Cara Cara fruit. The CsLCYb1 efifciently converted lycopene into the bicyclicβ-carotene in an Escherichia coli expression system, but the CsLCYb2 exhibited a lower enzyme activity and converted lycopene into theβ-carotene and the monocyclic γ-carotene. In tomato transformation studies, expression of CsLCYb1 under the control of the caulilfower mosaic virus (CaMV) 35S constitutive promoter resulted in a virtually complete conversion of lycopene intoβ-carotene, and the ripe fruits displayed a bright orange colour. However, the CsLCYb2 transgenic tomato plants did not show an altered fruit colour during development and maturation. In fruits of the CsLCYb1 transgenic plants, most of the lycopene was converted intoβ-carotene with provitamin A levels reaching about 700 µg g-1 DW. Unexpectedly, most transgenic tomatoes showed a reduction in total carotenoid accumulation, and this is consistent with the decrease in expression of endogenous carotenogenic genes in transgenic fruits. Collectively, these results suggested that the cloned CsLCYb1 and CsLCYb2 genes encoded two functional lycopene β-cyclases with different catalytic efifciency, and they may have potential for metabolite engineering toward altering pigmentation and enhancing nutritional value of food crops.

  15. Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck for selecting differentially expressed genes related to vascular hypertrophy.

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    Cheng-Quan Yang

    Full Text Available Corky split vein caused by boron (B deficiency in 'Newhall' Navel Orange was studied in the present research. The boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. Morphologic and anatomical surveys at four representative phases of corky split veins showed that the symptom was the result of vascular hypertrophy. Digital gene expression (DGE analysis was performed based on the Illumina HiSeq™ 2000 platform, which was applied to analyze the gene expression profilings of corky split veins at four morphologic phases. Over 5.3 million clean reads per library were successfully mapped to the reference database and more than 22897 mapped genes per library were simultaneously obtained. Analysis of the differentially expressed genes (DEGs revealed that the expressions of genes associated with cytokinin signal transduction, cell division, vascular development, lignin biosynthesis and photosynthesis in corky split veins were all affected. The expressions of WOL and ARR12 involved in the cytokinin signal transduction pathway were up-regulated at 1(st phase of corky split vein development. Furthermore, the expressions of some cell cycle genes, CYCs and CDKB, and vascular development genes, WOX4 and VND7, were up-regulated at the following 2(nd and 3(rd phases. These findings indicated that the cytokinin signal transduction pathway may play a role in initiating symptom observed in our study.

  16. Larvicidal activity of Syzygium aromaticum (L.) Merr and Citrus sinensis (L.) Osbeck essential oils and their antagonistic effects with temephos in resistant populations of Aedes aegypti

    Science.gov (United States)

    Araujo, Adriana Faraco de Oliveira; Ribeiro-Paes, João Tadeu; de Deus, Juliana Telles; Cavalcanti, Sócrates Cabral de Holanda; Nunes, Rogéria de Souza; Alves, Péricles Barreto; Macoris, Maria de Lourdes da Graça

    2016-01-01

    Environmentally friendly botanical larvicides are commonly considered as an alternative to synthetic larvicides against Aedes aegypti Linn. In addition, mosquito resistance to currently used larvicides has motivated research to find new compounds acting via different mechanisms of action, with the goal of controlling the spread of mosquitos. Essential oils have been widely studied for this purpose. This work aims to evaluate the larvicidal potential of Syzygium aromaticum and Citrus sinensis essential oils, either alone or in combination with temephos, on Ae. aegypti populations having different levels of organophosphate resistance. The 50% lethal concentration (LC50) of the essential oils alone and in combination with temephos and the influence of essential oils on vector oviposition were evaluated. The results revealed that essential oils exhibited similar larvicidal activity in resistant populations and susceptible populations. However, S. aromaticum and C. sinensis essential oils in combination with temephos did not decrease resistance profiles. The presence of the evaluated essential oils in oviposition sites significantly decreased the number of eggs compared to sites with tap water. Therefore, the evaluated essential oils are suitable for use in mosquito resistance management, whereas their combinations with temephos are not recommended. Additionally, repellency should be considered during formulation development to avoid mosquito deterrence. PMID:27384083

  17. Larvicidal activity of Syzygium aromaticum (L. Merr and Citrus sinensis (L. Osbeck essential oils and their antagonistic effects with temephos in resistant populations of Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Adriana Faraco de Oliveira Araujo

    2016-01-01

    Full Text Available Environmentally friendly botanical larvicides are commonly considered as an alternative to synthetic larvicides against Aedes aegypti Linn. In addition, mosquito resistance to currently used larvicides has motivated research to find new compounds acting via different mechanisms of action, with the goal of controlling the spread of mosquitos. Essential oils have been widely studied for this purpose. This work aims to evaluate the larvicidal potential of Syzygium aromaticum and Citrus sinensis essential oils, either alone or in combination with temephos, on Ae. aegypti populations having different levels of organophosphate resistance. The 50% lethal concentration (LC50 of the essential oils alone and in combination with temephos and the influence of essential oils on vector oviposition were evaluated. The results revealed that essential oils exhibited similar larvicidal activity in resistant populations and susceptible populations. However, S. aromaticum and C. sinensis essential oils in combination with temephos did not decrease resistance profiles. The presence of the evaluated essential oils in oviposition sites significantly decreased the number of eggs compared to sites with tap water. Therefore, the evaluated essential oils are suitable for use in mosquito resistance management, whereas their combinations with temephos are not recommended. Additionally, repellency should be considered during formulation development to avoid mosquito deterrence.

  18. Characterization of the Aldehydes and Their Transformations Induced by UV Irradiation and Air Exposure of White Guanxi Honey Pummelo (Citrus Grandis (L.) Osbeck) Essential Oil.

    Science.gov (United States)

    Li, Li Jun; Hong, Peng; Chen, Feng; Sun, Hao; Yang, Yuan Fan; Yu, Xiang; Huang, Gao Ling; Wu, Li Ming; Ni, Hui

    2016-06-22

    Aldehydes are key aroma contributors of citrus essential oils. White Guanxi honey pummelo essential oil (WPEO) was investigated in its aldehyde constituents and their transformations induced by UV irradiation and air exposure by GC-MS, GC-O, and sensory evaluation. Nine aldehydes, i.e., octanal, nonanal, citronellal, decanal, trans-citral, cis-citral, perilla aldehyde, dodecanal, and dodecenal, were detected in WPEO. After treatment, the content of citronellal increased, but the concentrations of other aldehydes decreased. The aliphatic aldehydes were transformed to organic acids. Citral was transformed to neric acid, geranic acid, and cyclocitral. Aldehyde transformation caused a remarkable decrease in the minty, herbaceous, and lemon notes of WPEO. In fresh WPEO, β-myrcene, d-limonene, octanal, decanal, cis-citral, trans-citral, and dodecenal had the highest odor dilution folds. After the treatment, the dilution folds of decanal, cis-citral, trans-citral, and dodecenal decreased dramatically. This result provides information for the production and storage of aldehyde-containing products. PMID:27226192

  19. Larvicidal activity of Syzygium aromaticum (L.) Merr and Citrus sinensis (L.) Osbeck essential oils and their antagonistic effects with temephos in resistant populations of Aedes aegypti.

    Science.gov (United States)

    Araujo, Adriana Faraco de Oliveira; Ribeiro-Paes, João Tadeu; Deus, Juliana Telles de; Cavalcanti, Sócrates Cabral de Holanda; Nunes, Rogéria de Souza; Alves, Péricles Barreto; Macoris, Maria de Lourdes da Graça

    2016-07-01

    Environmentally friendly botanical larvicides are commonly considered as an alternative to synthetic larvicides against Aedes aegypti Linn. In addition, mosquito resistance to currently used larvicides has motivated research to find new compounds acting via different mechanisms of action, with the goal of controlling the spread of mosquitos. Essential oils have been widely studied for this purpose. This work aims to evaluate the larvicidal potential of Syzygium aromaticum and Citrus sinensis essential oils, either alone or in combination with temephos, on Ae. aegypti populations having different levels of organophosphate resistance. The 50% lethal concentration (LC50) of the essential oils alone and in combination with temephos and the influence of essential oils on vector oviposition were evaluated. The results revealed that essential oils exhibited similar larvicidal activity in resistant populations and susceptible populations. However, S. aromaticum and C. sinensis essential oils in combination with temephos did not decrease resistance profiles. The presence of the evaluated essential oils in oviposition sites significantly decreased the number of eggs compared to sites with tap water. Therefore, the evaluated essential oils are suitable for use in mosquito resistance management, whereas their combinations with temephos are not recommended. Additionally, repellency should be considered during formulation development to avoid mosquito deterrence. PMID:27384083

  20. Effects of osmotic dehydration on quality of Citrus grandis(L.) Osbeck cv.Guanximiyou%不同渗透条件对琯溪蜜柚品质的影响

    Institute of Scientific and Technical Information of China (English)

    韩雅霏; 陈炜; 李俊先; 董全

    2011-01-01

    以琯溪蜜柚为原料,采用正交实验设计,研究不同渗透液含糖量(55%、60%、65%),不同渗透温度(50、55、60℃),不同料液比(1∶6、1∶8、1∶10)对蜜柚品质的影响。测定的指标包括失水率、抗坏血酸保存率、还原糖保存率、蔗糖增加率和总酸保存率。由多指标综合评分法得出的结果表明:渗透液含糖量65%,渗透温度60℃,料液比1∶10为最佳渗透脱水条件。不同渗透条件对蜜柚各营养指标均有影响,且呈现不同的影响效果。%The effect of osmotic dehydration on percentage of water loss,vitamin C retention,reducing sugar retention,sucrose increasing rate and total acid retention of Citrus grandis(L.)Osbeck cv.Guanximiyou(Guanxi pomelo)was investigated.Orthogonal array design was employed for evaluating the effects of sugar content(55%,60%,65%),temperature(50,55,60℃)and material-liquid ratio(1∶ 6,1∶ 8,1∶ 10)on quality of pomelo during osmotic dehydration.The results which were obtained by multi-indexes comprehensive method showed that the optimal osmotic dehydration condition were as follows:sugar solution 65%,temperature 60℃,material-liquid ratio 1∶ 10.Different conditions of osmotic dehydration had different effects on various parameters reflecting quality of pomelo.

  1. Comprehensive Analysis of Expressed Sequence Tags from the Pulp of the Red Mutant 'Cara Cara' Navel Orange(Citrus sinensis Osbeck)

    Institute of Scientific and Technical Information of China (English)

    Jun-Li Ye; An-Dan Zhu; Neng-Guo Tao; Qiang Xu; Juan Xu; Xiu-Xin Deng

    2010-01-01

    Expressed sequence tag(EST)analysis of the pulp of the red-fleshed mutant 'Cara Cara' navel orange provided a starting point for gene discovery and transcriptome survey during citrus fruit maturation. Interpretation of the EST datasets revealed that the mutant pulp transcriptome held a high section of stress responses related genes,such as the type Ⅲ metallothionein-like gene(6.0%),heat shock protein(2.8%),Cu/Zn superoxide dismutase(0.8%),late embryogenesis abundant protein 5(0.8%),etc. 133transcripts were detected to be differentially expressed between the red mutant and its orange-color wild genotype 'Washington' via digital expression analysis. Among them,genes involved in metabolism,defense/stress and signal transduction were statistical overrepresented. Fifteen transcription factors,composed of NAM,ATAF,and CUC transcription factor(NAC); myeloblastosis(MYB); myelocytomatosis(MYC); basic helix-loop-helix(bHLH); basic leucine zipper(bZIP)domain members,were also included. The data reflected the distinct expression profile and the unique regulatory module associated with these two genotypes. Eight differently expressed genes analyzed in digital were validated by quantitative realtime polymerase chain reaction. For structural polymorphism,both simple sequence repeats and single nucleotide polymorphisms(SNP)loci were surveyed; dinucleotide presentation revealed a bias toward AG/GA/TC/CT repeats(52.5%),against GC/CG repeats(0%). SNPs analysis found that transitions(73%)outnumbered transversions(27%). Seventeen potential cultivar-specific and 387 heterozygous SNP loci were detected from 'Cara Cara' and 'Washington' EST pool.

  2. A Proteomic Analysis of the Chromoplasts Isolated from Sweet Orange Fruits [Citrus sinensis (L.) Osbeck] Revealed Clues to Their Ripening Event

    Institute of Scientific and Technical Information of China (English)

    Yunliu Zeng; Zhiyong Pan; Yuduan Ding; Andan Zhu; Hongbo Cao; Qiang Xu; Xiuxin Deng

    2012-01-01

    Carotenoid biosynthesisis believed to occur in chromoplasts,which are non-photosynthetic plastids often present in flowers and fruits.We report a comprehensive proteomic analysis of the chromoplasts,which were purified from sweet orange by a novel protocol using Nycodenz density gradient centrifugation.Western blot analysis using polyclonal antibodies against marker proteins for different cell compartments suggested the chromoplast extraction were of high purity.Systematic analysis of the chromoplast proteome identified 493 proteins,of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses.Based on the predicted functions of these identified plastid proteins,a large proportion (~60%) of the chromoplast proteome of sweet orange is constituted by proteins involved in carbohydrate metabolism,amino acid/protein synthesis,and secondary metabolism.Of note,HDS (hydroxymethylbutenyl 4-diphosphate synthase),PAP (plastid-lipid-associated protein),and psHSPs (plastid small heat shock proteins) involved in the synthesis or storage of carotenoid and stress response are among the most abundant proteins identified.A comparison of chromoplast proteomes between sweet orange and tomato suggested a high level of conservation in a broad range of metabolic pathways.However,the citrus chromoplast was characterized by more extensive carotenoid synthesis,extensive amino acid synthesis without nitrogen assimilation,and evidence for lipid metabolism concerning jasmonic acid synthesis.In conclusion,this study provides an insight into the major metabolic pathways as well as some unique characteristics of the sweet orange chromoplasts at the whole proteome level.

  3. Influencia del porta-injertos y la época de cosecha sobre la calidad de pomelo (Citrus paradisis Macfad variedad Rio Red

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    Andrés José Armadans Rojas

    2014-04-01

    Full Text Available En el campo experimental de la Facultad de Ciencias Agrarias de la Universidad Nacional de Asunción San Lorenzo, Paraguay, se estudió la influencia del tipo de porta-injerto y la época de cosecha sobre la calidad del fruto del pomelo rosado (Citrus paradisis. Macfad var. Rio Red. Se utilizó un diseño experimental de bloques completos al azar con arreglo en parcelas subdivididas, siendo la parcela principal las épocas (abril, mayo y junio de cosecha y la subparcela los porta-injertos [tangelo Orlando -Citrus tangerina Hort. ex Tanaka x Citrus paradisi Macfad., limón rugoso -Citrus jambhiri Luch, lima Rangpur -Citrus limonia y Citrange C35 -Poncirus trifoliata (L. Raf. x Citrus sinensis (L. Osbeck cv. Ruby en cuatro repeticiones. Se cosecharon 10 frutos por unidad experimental y se evaluaron los caracteres morfológicos: diámetro y forma del fruto, y los porcentajes de jugo, bagazo, sólidos solubles totales,acidez titulable y relación o cociente entre sólidos solubles totales: acidez. Los resultados mostraron la influencia de los porta-injertos sobre la calidad del fruto del pomelo Rio Red. El limón rugoso fue el porta-injerto que presentó el menor porcentaje de jugo (47.33% y el mayor en bagazo (48.72%, sólido soluble (10.54% y acidez (1.42%. Mientras que el mayor porcentaje de jugo (52.76 y 50.75% se presentó en los frutos cosechados en abril y mayo. El mayor porcentaje de acidez se presentó en abril (9.53%.

  4. Balanço hídrico no solo para porta-enchertos de citros em ecossistema de tabuleiro costeiro Water balance in soil for citrus rootstocks in the brazilian coastal tableland ecosystem

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    Fernando Luis Dultra Cintra

    2000-04-01

    Full Text Available A má distribuição das chuvas e a existência de camadas coesas em muitos solos dos tabuleiros promovem, muitas vezes, alterações importantes no regime hídrico do solo e nas taxas de evapotranspiração das culturas exploradas nesse ecossistema. Objetivou-se, com este trabalho, conhecer, através do balanço hídrico, como esses processos ocorrem e, também, contribuir para o estabelecimento de práticas de manejo visando ao melhor uso das reservas de água no solo. Todos os componentes do balanço foram medidos, à exceção da evapotranspiração, que foi calculada. Os resultados obtidos permitiram concluir que a maior demanda hídrica da laranjeira aconteceu nos meses de outubro e novembro e que, com base na taxa de evapotranspiração, a Tangerina Cleópatra (Citrus reshni Hort. ex Tan. foi o porta-enxerto menos adaptado e o Limão Cravo (Citrus limonia Osbeck o que apresentou as melhores características de adaptação à área estudada.The irregular rain distribution and the existence of hardened layers in several soils of the tablelands promote, sometimes, important changes in the soil water regime and in the rates of evapotranspiration of the crops utilized in that ecosystem. The objective of this work was to know, by means of the water balance, how these processes occur in the studied area and to contribute to the establishment of management practices in order to make better use of the soil water. All components of the equations were measured, with exception of the evapotranspiration which was calculated. According to the results, it could be concluded that the orange tree had the maximum water consumption during the months of October and November and that, in terms of the rootstocks during these periods of great water consumption, the "Tangerina Cleópatra" (Citrus reshni Hort. former Tan. was the less adapted and the "Limão Cravo" (Citrus limonia Osbeck presented the best adaptation characteristics to the studied area.

  5. Optimization design based on kinetic model and extraction process of pectin from pomelo (Citrus grandis L.Osbeck) fruit peel%柚果皮果胶浸提过程及动力学分析优化设计

    Institute of Scientific and Technical Information of China (English)

    周存山; 余筱洁; 马海乐; 王允祥; 韩平

    2013-01-01

    以琯溪蜜柚果皮为原料,采用果胶酸解浸提法,基于果胶在浸提过程中包括原果胶转变为果胶、果胶从组织扩散到溶剂、果胶大分子降解等进程,以同步串联反应法构建果胶浸提动力学模型,获得基于浸提工艺优化和果胶同步降解影响的浸提动力学方程,进而进行模型验证分析及参数优化.有效性(残差分析及F检验)检验表明:该模型可应用于分析柚果皮果胶浸提动力学过程,在温度70 ~ 90℃,浸提过程表观活化能Ea为31.34kJ· mol-1;优化分析获得在浸提液pH =2.0,浸提温度90℃,浸提时间90.25 min,料液质量与体积比为1:40时,柚果皮果胶浸提得率11.83%,此结果与正交设计优化结果吻合.%The pectin extraction of pomelo (Citrus grandis L. Osbeck) fruit peel was investigated by the method of acid hydrolysis. The kinetic model of the processes of protopectin hydrolysis to pectin, pectin diffusion from tissue to solvent, degradation of some pectin macromolecules was established by synchronous series reaction method to estimate the most important mass transfer coefficients. Based on the effects of degradation and extraction of pectin, and optimization of simultaneous extraction conditions, the kinetic equations were obtained to realize model validation and optimization parameter. The validations of residual error analysis and F test show that the model can be applied to the kinetic analysis of pectin extraction from pomelo fruit peel. The apparent activation energy of extraction process is 31. 34 kJ·mol-1 at temperature of 70 - 90 ℃ . According to the optimization analysis, the pectin extraction yield from pomelo fruit peel is 11. 83% of dried peel at the optimized conditions with pH value of 2. 0,extraction temperature of 90 ℃ , extraction time of 90 min and solid to liquid ration of 1 : 40. The optimization condition and the maximum yield from kinetic model are confirmed by the results from orthogonal experiment.

  6. Bacteria as growth-promoting agents for citrus rootstocks.

    Science.gov (United States)

    Giassi, Valdionei; Kiritani, Camila; Kupper, Katia Cristina

    2016-09-01

    The microbial community plays an essential role in maintaining the ecological balance of soils. Interactions between microorganisms and plants have a major influence on the nutrition and health of the latter, and growth-promoting rhizobacteria can be used to improve plant development through a wide range of mechanisms. Therefore, the objective of the present study was to evaluate bacteria as growth-promoting agents for citrus rootstocks. A total of 30 bacterial isolates (11 of Bacillus spp., 11 actinobacteria, and 8 lactic acid bacteria) were evaluated in vitro for indoleacetic acid production, phosphate solubilization, and nitrogen (N) fixation. In vivo testing consisted of growth promotion trials of the bacterial isolates that yielded the best results on in vitro tests with three rootstocks: Swingle citrumelo [Citrus×paradisi Macfad cv. Duncan×Poncirus trifoliata (L.) Raf.], Sunki mandarin (Citrus sunki Hort. ex Tan), and rangpur (Citrus×limonia Osbeck). The parameters of interest were height, number of leaves, stem diameter, shoot and root dry mass, and total dry mass at 150days after germination. The results showed that most bacterial isolates were capable of IAA production. Only one lactic acid bacterium isolate (BL06) solubilized phosphate, with a high solubilization index (PSI>3). In the actinobacteria group, isolates ACT01 (PSI=2.09) and ACT07 (PSI=2.01) exhibited moderate phosphate-solubilizing properties. Of the Bacillus spp. isolates, only CPMO6 and BM17 solubilized phosphate. The bacterial isolates that most fixated nitrogen were BM17, ACT11, and BL24. In the present study, some bacteria were able to promote growth of citrus rootstocks; however, this response was dependent on plant genotype and isolate. Bacillus spp. BM16 and CPMO4 were able to promote growth of Swingle citrumelo. In Sunki mandarin plants, the best treatment results were obtained with BM17 (Bacillus sp.) and ACT11 (actinobacteria). For Rangpur lime rootstock, only BM05 (Bacillus sp

  7. Bacteria as growth-promoting agents for citrus rootstocks.

    Science.gov (United States)

    Giassi, Valdionei; Kiritani, Camila; Kupper, Katia Cristina

    2016-09-01

    The microbial community plays an essential role in maintaining the ecological balance of soils. Interactions between microorganisms and plants have a major influence on the nutrition and health of the latter, and growth-promoting rhizobacteria can be used to improve plant development through a wide range of mechanisms. Therefore, the objective of the present study was to evaluate bacteria as growth-promoting agents for citrus rootstocks. A total of 30 bacterial isolates (11 of Bacillus spp., 11 actinobacteria, and 8 lactic acid bacteria) were evaluated in vitro for indoleacetic acid production, phosphate solubilization, and nitrogen (N) fixation. In vivo testing consisted of growth promotion trials of the bacterial isolates that yielded the best results on in vitro tests with three rootstocks: Swingle citrumelo [Citrus×paradisi Macfad cv. Duncan×Poncirus trifoliata (L.) Raf.], Sunki mandarin (Citrus sunki Hort. ex Tan), and rangpur (Citrus×limonia Osbeck). The parameters of interest were height, number of leaves, stem diameter, shoot and root dry mass, and total dry mass at 150days after germination. The results showed that most bacterial isolates were capable of IAA production. Only one lactic acid bacterium isolate (BL06) solubilized phosphate, with a high solubilization index (PSI>3). In the actinobacteria group, isolates ACT01 (PSI=2.09) and ACT07 (PSI=2.01) exhibited moderate phosphate-solubilizing properties. Of the Bacillus spp. isolates, only CPMO6 and BM17 solubilized phosphate. The bacterial isolates that most fixated nitrogen were BM17, ACT11, and BL24. In the present study, some bacteria were able to promote growth of citrus rootstocks; however, this response was dependent on plant genotype and isolate. Bacillus spp. BM16 and CPMO4 were able to promote growth of Swingle citrumelo. In Sunki mandarin plants, the best treatment results were obtained with BM17 (Bacillus sp.) and ACT11 (actinobacteria). For Rangpur lime rootstock, only BM05 (Bacillus sp

  8. Aproveitamento da casca de citros na perspectiva de alimentos: prospecção da atividade antibacteriana Utilization of citrus by-products in food perspective: screening of antibacterial activity

    Directory of Open Access Journals (Sweden)

    Carin Gerhardt

    2012-05-01

    Full Text Available Os citros são as frutas mais produzidas e consumidas no mundo. O Brasil ocupa primeiro lugar na produção mundial e na exportação de suco de laranja, sendo o Estado do Rio Grande do Sul um importante produtor. Ao longo do cultivo e do processamento dos citros, são geradas toneladas de resíduos de baixo valor comercial, mas com grande potencial de aproveitamento dentro da indústria de alimentos. Esses resíduos possuem elevados teores de nutrientes, pigmentos e componentes bioativos, bem como possuem baixa toxicidade e baixo custo. Há evidências de que a casca de diferentes espécies de citros possui princípios ativos antibacterianos e antifúngicos. O objetivo deste trabalho, portanto, foi verificar a atividade antibacteriana de extratos alcoólicos da casca de citros na perspectiva da desinfecção e da conservação de alimentos, propondo alternativas sustentáveis e naturais voltadas a consumidores cada vez mais preocupados com sua saúde. Foram obtidos extratos alcoólicos da casca crua de bergamota-ponkan (Citrus reticulata Blanco, pomelo (Citrus maxima (Burm. Merr. e limão-bergamota (Citrus limonia Osbeck ou limão-cravo maduros, provenientes de cultivo agroecológico, cujas atividades antibacterianas foram avaliadas quanto à Concentração Inibitória Mínima (CIM e à Concentração Bactericida Mínima (CBM frente a cinco diferentes bactérias. O extrato de limão-bergamota apresentou a melhor atividade antibacteriana, apresentando CIM em torno de 24 mg.mL-1 e CBM de 42 mg.mL-1 para as bactérias mais resistentes. A bactéria mais sensível a todos os extratos foi Pseudomonas aeruginosa, com CIM entre 16 e 36 mg.mL-1 e CBM entre 28 e 49 mg.mL-1. Os extratos inibiram ou inativaram na sua totalidade as bactérias testadas, indicando a possibilidade de se tornarem alternativas naturais na desinfecção e na conservação de alimentos.Citrus are the most produced fruits in the world. Brazil ranks first in global production and

  9. Ingestão de seiva do xilema de laranjeiras 'Pêra' e 'Valência' (Citrus sinensis (L. Osbeck sadias e infectadas por Xylella fastidiosa, pelas cigarrinhas vetoras Oncometopia facialis e Dilobopterus costalimai (Hemiptera: Cicadellidae Xylem sap ingestion form healthy "Pera" and "Valencia" sweet orange (Citrus sinensis (L. Osbeck and infected ones by Xylella fastidiosa, Oncometopia facialis and Dilobopterus costalimai (Hemiptera: Cicadellidae

    Directory of Open Access Journals (Sweden)

    Luiz Henrique Montesino

    2006-08-01

    Full Text Available Estudou-se o efeito da infecção pela bactéria Xylella fastidiosa, agente causal da Clorose Variegada dos Citros (CVC, sobre a taxa de ingestão de seiva do xilema de plantas cítricas por duas espécies de cigarrinhas vetoras (Hemiptera: Cicadellidae. Foram utilizados pés-francos de laranjeira-doce (Citrus sinensis das variedades 'Pêra' e 'Valência', infectadas por X. fastidiosa da linhagem 9a5c, por meio de inoculação mecânica. Os insetos utilizados nos experimentos foram coletados em campo, sendo um representante da Tribo Cicadellini (Dilobopterus costalimai e um da Proconiini (Oncometopia facialis. A taxa de ingestão de seiva do xilema por O. facialis foi quantificada nos ramos das plantas e a de D. costalimai nas folhas e ramos, por meio da avaliação do volume do líquido (honeydew excretado por unidade de tempo. O consumo pela cigarrinha O. facialis nas plantas doentes foi menor do que nas plantas sadias. Na variedade 'Pêra' doente, o consumo foi baixo, não permitindo a quantificação da seiva eliminada. Na 'Pêra' sadia e na 'Valência' doente e sadia, O. facialis apresentou valores expressivos de excreção, com maior alimentação no período diurno. Nas plantas sadias das duas variedades, o consumo pela cigarrinha D. costalimai foi maior do que nas plantas com CVC. Comparando-se as variedades, o consumo foi superior na variedade 'Valência', e, em relação às partes da planta, folha e ramo, a taxa de ingestão foi maior no ramo das duas variedades, apresentando consumo maior no período diurno.It was studied the effect of Xylella fastidiosa infection, causal agent of Citrus Variegated Chlorosis (CVC, on the xylem sap ingestion rate of citrus plants by two sharpshooters species (Hemiptera: Cicadellidae. Seedlings of sweet orange Pera and Valencia (Citrus sinensis were used and infected by X. fastidiosa, strain 9a5c, obtained by mechanical inoculation. The insects used in the experiments were collected in the field, one

  10. Efeitos de mbta [cloridrato de n,n-dietil-2-(4-metilbenziloxi etilamina] na produtividade e na qualidade dos frutos da laranjeira 'pêra' (Citrus sinensis L. Osbeck Effects of mbta [n,n-diethyl-2-(4-metylbenzyloxy ethylamine hydrochloride] on yield and fruit quality of 'pêra' sweet orange (Citrus sinensis L. Osbeck

    Directory of Open Access Journals (Sweden)

    Chryz Melinski Serciloto

    2008-09-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos do biorregulador MBTA [cloridrato de N,N-dietil-2-(4-metilbenziloxi etilamina] aplicado em diferentes épocas e concentrações na produtividade e qualidade dos frutos da laranjeira 'Pêra'. Em duas safras consecutivas, o MBTA foi aplicado em três diferentes concentrações (8; 16 e 32 mg L-1 e em duas diferentes fases fenológicas (25% e 100% de flores abertas, em árvores cítricas adultas, utilizando um volume de 7 litros de solução por planta, acompanhado do adjuvante Silwett L-77 0,05%. Foram amostrados 20 frutos por planta, em quatro diferentes épocas estudadas, para determinar o teor de sólidos solúveis (SS; acidez titulável (AT; quantidade de sólidos solúveis por caixa de 40,8 kg; pH; rendimento de suco; "ratio" (relação SS/AT, e a massa média dos frutos. Os efeitos do MBTA variaram de acordo com a concentração aplicada e com a fase fenológica de aplicação. O MBTA, na concentração de 8 mg L-1, aplicado com 25% das flores abertas, incrementou o teor de sólidos solúveis, a acidez, a quantidade de sólidos solúveis por caixa de 40,8 kg e a produtividade. Esse mesmo tratamento também reduziu a massa média dos frutos e não alterou o rendimento de suco e o "ratio" do suco dos frutos. O incremento médio foi de 0,49 a 0,65% na concentração de sólidos solúveis, de 0,11 a 0,13 kg na quantidade de sólidos solúveis por caixa de 40,8 kg e de 20,4 kg/planta na produtividade.The purpose of this research was to evaluate the effects of MBTA [N,N-diethyl-2-(4-metylbenzyloxy ethylamine hydrochloride] bioregulator applied on different times and concentrations on the yield and fruit quality of 'Pera' sweet orange. In two consecutive harvest seasons, the MBTA was sprayed in three different concentrations (8; 16 and 32 mg L-1 and in two different phenological phases (25% and 100% open flowers in citrus mature trees, using 7 L of spray per tree added with Silwett L-77 adjuvant at 0

  11. Effect of Cold Storage and On-tree Storage on Fruit Carotenoid Composition and Content of ' Redflesh navel orange' (Citrus sinensis Osbeck)%冷藏和留树保鲜对红肉脐橙果实类胡萝卜素种类和含量的影响

    Institute of Scientific and Technical Information of China (English)

    王璠; 伊华林; 郭琳琳

    2007-01-01

    红肉脐橙(Citrus sinensis Osbeck cv.‘Redflesh navel orange’)丰产优质,是唯一果肉粉红且着色均匀的甜橙品种,源自华盛顿脐橙的芽变,20世纪90年代初由美国引进,于2001年被湖北省农作物品种审定委员会认定为新品种。已有研究证明:红肉脐橙果肉中的主要色素为番茄红素和β-胡萝b素。但通过对红肉脐橙果实进行冷藏及留树保鲜处理,发现其果实品质及果皮、果肉颜色与明亮度变化有些不同。

  12. 通过机械加工获得的甜橙油(ISO3140:2005)%The International Standard of Crude or Rectified Oil of sweet orange(Citrus sinensis(L.)Osbeck),obtained by mechanical treatment(ISO 3140:2005)

    Institute of Scientific and Technical Information of China (English)

    徐易

    2007-01-01

    @@ 1 目的 本标准规定了甜橙油[Citrus sinensis(L.)Osbeck]的某些特性,以便对其 质量进行评估. 2 规范性引用文件 下列规范性文件所包含的条款,通过在本标准中的引用而成为本标准的条款.凡是注日期的引用文件,其随后所有的修改单(不包括勘误的内容)或修订版均不适用于本标准,然而鼓励根据本标准达成协议的各方研究是否可使用这些标准的最新版本.凡是不注日期的引用文件,其最新版本适用于本标准.

  13. Digital Gene Expression Analysis of Corky Split Vein Caused by Boron Deficiency in ‘Newhall’ Navel Orange (Citrus sinensis Osbeck) for Selecting Differentially Expressed Genes Related to Vascular Hypertrophy

    OpenAIRE

    Yang, Cheng-Quan; Liu, Yong-Zhong; An, Ji-Cui; Li, Shuang; Jin, Long-Fei; Zhou, Gao-Feng; Wei, Qing-Jiang; Yan, Hui-Qing; Wang, Nan-Nan; Fu, Li-Na; Liu, Xiao; Hu, Xiao-Mei; Yan, Ting-Shuai; Peng, Shu-Ang

    2013-01-01

    Corky split vein caused by boron (B) deficiency in ‘Newhall’ Navel Orange was studied in the present research. The boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. Morphologic and anatomical surveys at four representative phases of corky split veins showed that the symptom was the result of vascular hypertrophy. Digital gene expression (DGE) analysis was performed based on the Illumina HiSeq™ 2000 platform, which was applied to analyze the gene expression profi...

  14. Cloning and Characterization of a Novel cDNA Encoding Late Embryogenesis-Abundant Protein 5 Like (LEA-5) Gene from Cara Cara Navel Orange Fruit(Citrus sinensis Osbeck)

    Institute of Scientific and Technical Information of China (English)

    TAO Neng-guo; YE Jun-li; XU Juan; DENG Xiu-xin

    2006-01-01

    LEA5 gene was postulated related with both stress and hormone responses. In an attempt to find genes exclusively expressed during fruit ripening of Cara Cara navel orange, a novel cDNA clone encoding late embryogenesis-abundant protein 5 like gene (CitLEA5-1) was obtained. It was 582 bp in length, containing 97 deduced amino acids. Compared with the stress-induced LEA5 from leaves of Citrus sinensis, CitLEA5-1 had a shorter 3' untranslated region (UTR). Semiquantitative RT-PCR analysis revealed that CitLEA5-1 was transcriptional regulated during fruit ripening of Cara Cara navel orange.

  15. Efficient Isolation of RNA from Fruit Peel and Pulp of Ripening Navel Orange (Citrus sinensis Osbeck)%一种适合于成熟脐橙果皮和果肉的RNA提取方法

    Institute of Scientific and Technical Information of China (English)

    刘永忠; 刘庆; 陶能国; 邓秀新

    2006-01-01

    An efficient RNA isolation method was established in the present paper. RNA extracted from peel and pulp collected at different ripening time was successfully used for reverse transcription-PCR (RT-PCR) without DNase treatment, complementary DNA-amplified fragment length polymorphism (cDNA-AFLP), and RNA blotting. The A260/A230ratios were higher than 2. 0, and the A260/A280 ratios ranged from 1.65 to 1.92. In addition, the protocol is safe, convenient and proved to be widely applicable, since it was successfully employed for RNA extraction from citrus leaves, immature fruit, Poncirus seedling and citrus callus as well.%研究了一种适合脐橙果实成熟过程中有效的RNA提取方法.结果表明利用该方法从不同成熟时期果实的果皮和果肉中提取的RNA可以有效用于RT-PC,cDNA-AFLP和RNA杂交.其A260/A230的比值超过2.0,A260/A280的比值在1.65-1.92的范围之间.另外该方法也证明可以广泛用于柑橘叶片,未成熟的幼果,枳壳幼苗和柑橘愈伤组织的RNA提取,是一种安全、方便和适用性较广的RNA提取方法.

  16. Occurrence, molecular characterisation, and pathogenicity of Neoscytalidium dimidiatum on Citrus in Italy

    NARCIS (Netherlands)

    Polizzi, G.; Aiello, D.; Castello, I.; Vitale, A.; Groenewald, J.Z.; Crous, P.W.; Gentile, A.; La Malfa, S.

    2011-01-01

    During 2008 and 2009, a new disease was noticed in eastern Sicily (Italy) in two re-grafted citrus orchards (Citrus sinensis (L.) Osbeck) on sour orange rootstock. Symptoms consisted of blight of vigorously growing shoots and a sooty canker on shoots and rootstock trunks. A Scytalidium-like fungus w

  17. Occurrence, molecular characterisation, and pathogenicity of Neoscytalidium dimidiatum on citrus in Italy

    NARCIS (Netherlands)

    Polizzi, G.; Aiello, D.; Castello, I.; Vitale, A.; Groenewald, J.Z.; Crous, P.W.

    2011-01-01

    During 2008 and 2009, a new disease was noticed in eastern Sicily (Italy) in two re-grafted citrus orchards (Citrus sinensis (L.) Osbeck) on sour orange rootstock. Symptoms consisted of blight of vigorously growing shoots and a sooty canker on shoots and rootstock trunks. A Scytalidium-like fungus w

  18. First report of Xiphinema rivesi Dalmasso, 1969 on citrus in northern Egypt

    Science.gov (United States)

    During a nematode survey in 2012-2013 in EL-Nobarria, EL-Behera governorate, northern Egypt, specimens of dagger nematode (Xiphinema sp.) were collected from soil around the rhizosphere of citrus trees (Citrus sinensis (L.) Osbeck) exhibiting poor growth and low yield. The morphology of females esta...

  19. Involvement of an ethylene response factor in chlorophyll degradation during citrus fruit degreening

    Science.gov (United States)

    Chlorophyll degradation naturally occurs during plant senescence. However, in fruit such as citrus, it is a positive characteristic, as degreening is an important colour development contributing to fruit quality. In the present work, Citrus sinensis Osbeck, cv. Newhall fruit was used as a model for ...

  20. Anti-Inflammatory Properties and Chemical Characterization of the Essential Oils of Four Citrus Species.

    Directory of Open Access Journals (Sweden)

    Jorge Luis Amorim

    Full Text Available Citrus fruits have potential health-promoting properties and their essential oils have long been used in several applications. Due to biological effects described to some citrus species in this study our objectives were to analyze and compare the phytochemical composition and evaluate the anti-inflammatory effect of essential oils (EO obtained from four different Citrus species. Mice were treated with EO obtained from C. limon, C. latifolia, C. aurantifolia or C. limonia (10 to 100 mg/kg, p.o. and their anti-inflammatory effects were evaluated in chemical induced inflammation (formalin-induced licking response and carrageenan-induced inflammation in the subcutaneous air pouch model. A possible antinociceptive effect was evaluated in the hot plate model. Phytochemical analyses indicated the presence of geranial, limonene, γ-terpinene and others. EOs from C. limon, C. aurantifolia and C. limonia exhibited anti-inflammatory effects by reducing cell migration, cytokine production and protein extravasation induced by carrageenan. These effects were also obtained with similar amounts of pure limonene. It was also observed that C. aurantifolia induced myelotoxicity in mice. Anti-inflammatory effect of C. limon and C. limonia is probably due to their large quantities of limonene, while the myelotoxicity observed with C. aurantifolia is most likely due to the high concentration of citral. Our results indicate that these EOs from C. limon, C. aurantifolia and C. limonia have a significant anti-inflammatory effect; however, care should be taken with C. aurantifolia.

  1. Anti-Inflammatory Properties and Chemical Characterization of the Essential Oils of Four Citrus Species.

    Science.gov (United States)

    Amorim, Jorge Luis; Simas, Daniel Luiz Reis; Pinheiro, Mariana Martins Gomes; Moreno, Daniela Sales Alviano; Alviano, Celuta Sales; da Silva, Antonio Jorge Ribeiro; Fernandes, Patricia Dias

    2016-01-01

    Citrus fruits have potential health-promoting properties and their essential oils have long been used in several applications. Due to biological effects described to some citrus species in this study our objectives were to analyze and compare the phytochemical composition and evaluate the anti-inflammatory effect of essential oils (EO) obtained from four different Citrus species. Mice were treated with EO obtained from C. limon, C. latifolia, C. aurantifolia or C. limonia (10 to 100 mg/kg, p.o.) and their anti-inflammatory effects were evaluated in chemical induced inflammation (formalin-induced licking response) and carrageenan-induced inflammation in the subcutaneous air pouch model. A possible antinociceptive effect was evaluated in the hot plate model. Phytochemical analyses indicated the presence of geranial, limonene, γ-terpinene and others. EOs from C. limon, C. aurantifolia and C. limonia exhibited anti-inflammatory effects by reducing cell migration, cytokine production and protein extravasation induced by carrageenan. These effects were also obtained with similar amounts of pure limonene. It was also observed that C. aurantifolia induced myelotoxicity in mice. Anti-inflammatory effect of C. limon and C. limonia is probably due to their large quantities of limonene, while the myelotoxicity observed with C. aurantifolia is most likely due to the high concentration of citral. Our results indicate that these EOs from C. limon, C. aurantifolia and C. limonia have a significant anti-inflammatory effect; however, care should be taken with C. aurantifolia.

  2. Respective Effects of Post-harvest Citrus Oil Induction and Vibration Stress Treatment on Oleocellosis in Jincheng Orange Peel(Citrus sinensis Osbeck cv. Jincheng)%采后橘油诱导及振动胁迫对贮藏期锦橙果皮油胞病的影响

    Institute of Scientific and Technical Information of China (English)

    刘丽丹; 吴日章; 曾凯芳

    2011-01-01

    The respective effects of post-harvest citrus oil induction and vibration stress treatment on oleocellosis in Jincheng orange peel were studied based on collapse index and color index.Oleocellosis in Jincheng orange peel showed a gradual increase as the concentration of citrus oil increased.The most serious oleocellosis in citrus peel could arise from 100% citrus oil treatment.In addition,mechanical vibration stress also could induce oleocellosis in Jincheng orange peel.Mechanical vibration stress at 500 r/min revealed the most obvious effect on oleocellosis formation.Moreover,Jincheng oranges treated with citrus oil or mechanical vibration stress were also used to explore the effect of storage temperature on oleocellosis in their peels.As a result,Jincheng oranges stored at 20 or 5 ℃had the severest change in oleocellosis.However,Jincheng oranges had the slightest change in oleocellosis during 5 ℃ storage.Therefore,these results can provide a theoretical reference for exploring the mechanisms and control strategies of oleocellosis.%以果皮塌陷指数和变色指数为测定指标,研究橘油及振动胁迫处理对锦橙果皮油胞病的影响。结果表明:随橘油体积分数增大果皮油胞病逐渐加重,并以100%橘油处理对锦橙果皮油胞病的诱导效果最明显;锦橙果皮油胞病发病程度随着振动胁迫强度增大而加重,其中500r/min的诱导效果最显著。另外,在橘油和振动胁迫处理后的果实在不同温度下贮藏时,发现20℃贮藏的锦橙果皮油胞病最为严重,0℃冷害温度也能加剧果实油胞病的发生,5℃贮藏的锦橙果皮油胞病最轻微。研究结果为进一步研究柑橘油胞病的发生机理及防控措施提供理论依据。

  3. Organic Acid Content, Microbial Quantity and Enzyme Activity in Rhizosphere Soil of Four Citrus Rootstocks Under Different Phosphorus Levels%不同施磷水平下4种柑橘砧木的根际土壤有机酸、微生物及酶活性

    Institute of Scientific and Technical Information of China (English)

    罗燕; 樊卫国

    2014-01-01

    酸含量显著增多,土壤生物活性增强;在酸性黄壤上,宜昌橙能较好地适应低磷土壤环境。%[Objective] The effects of different phosphorus (P) levels on the composition and content of organic acids, the population and quantity of microbe and enzyme activities in rhizosphere soil of different citrus rootstocks were explored for providing a scientific basis of enriching the theory about phosphorus nutrition and ecology of citrus and discovering the citrus rootstock with specific nutrient characteristics.[Method]A pot experiment was adopted to study the ability to phosphorus stress, the composition and content of organic acids, the population and quantity of microbe and enzyme activities in rhizosphere soil of Citrus ichangensis Swing., C.aurantium L., C.limonia Osbeck and Poncirus trifoliata Raf. under different P levels, and their correlation was analyzed.[Result]The dry matter accumulation of C.aurantium L., C.limonia Osbeck and P. trifoliata Raf. significantly increased with the rised P levels. There was no obvious effect of different P levels on the biomass of C. ichangensis Swing.. The tolerance to low-P stress was in the order of C. ichangensis Swing.>C.limonia Osbeck>C.aurantium L.>P. trifoliata Raf. The composition and content of organic acids in rhizosphere soil of different citrus rootstocks were significantly different, and the oxalic acid, succinic acid and acetic acid were the main organic acids.The total content of organic acids and the content of oxalic acid, malonic acid in rhizosphere soil of different citrus rootstocks and the content of succinic acid in rhizosphere soil of C.ichangensis Swing. and C.aurantium L. significantly increased with the reduced P levels. Under phosphate starvation, the amount of organic acid in rhizosphere soil of C.ichangensis Swing. was significantly higher than other citrus rootstocks. Bacteria were dominant, followed by actinomycetes, and fungi were the least. The quantity of bacteria

  4. Citrus orchard planted with no tillage and conventional systemsHuerto de cítricos plantado con sistema de cero labranza y sistema convencional

    Directory of Open Access Journals (Sweden)

    Carmen Silvia Vieira Janeiro Neves

    2010-02-01

    Full Text Available No tillage system has brought many advantages to soil protection, without reducing the yield of annual crops. This system has been adapted for Citrus orchards, with the plantation of trees in furrows opened in areas previously occupied by pastures. This work had as objective to evaluate tree development, root system, yield, and soil characteristics in an orchard planted in two systems. The study was made in Parana State, Brazil, in a medium texture oxisol with ‘Valência’ orange (Citrus sinensis L. trees with Rangpur lime rootstock (Citrus limonia Osbeck. The treatments were no tillage (furrowing and plantation and conventional plantation (hole area plowing and disk harrowing before furrowing and plantation. The yield was 86 - 129 kg of fruits per plant, without significant difference between treatments. The plantation systems either do not affect the development of the plants, the amount of roots and the fruit quality. In the conventional plantation system the soil has greater resistance to penetration in inter rows than in the zero tillage system plantation.El sistema de cero labranza ha traído muchas ventajas en la protección del suelo, sin perjudicar el rendimiento de los cultivos anuales. Este sistema ha sido adaptado para los huertos citrícolas, realizándose la plantación en surcos abiertos en terrenos anteriormente ocupados por pastizales. El trabajo tuvo como objetivo evaluar el desarrollo de las plantas y del sistema radicular, producción, características del suelo en un huerto plantado en dos sistemas de labranza. El estudio fue realizado en el Noroeste de Paraná, en un oxisol con textura media, en plantas de naranja ‘Valência’ con patrón de lima Rangpur. Los tratamientos evaluados fueron: cero labranza (apertura de surcos y plantación y plantación convencional (arado de discos y grada, seguido de apertura de surcos y plantación. La producción fue de 86 a 129 kg de frutos por planta, sin diferencia significativa

  5. In vitro organogenesis in some citrus species

    Directory of Open Access Journals (Sweden)

    Evandro Henrique Schinor

    2011-06-01

    Full Text Available In vitro organogenesis of Citrus was studied for the genotypes Citrus sinensis cv. 'Natal', C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP - 0.0; 0.5; 1.0; 1.5 or 2.0 mg L-1. For the recalcitrant genotypes C. limonia and C. aurantium the in vitro organogenesis was also studied with internodal segments-derived explants, cultured in MT salts and vitamins medium supplemented with 0; 0.5; 1.0; 2.0, or 4.0 mg L-1 of BAP. The efficiency of culture medium supplementation with the combination of BAP (0.0; 1.0, or 2.0 mg L-1 and NAA (1-naphthaleneacetic acid - 0.0; 0.3, or 0.5 mg L-1 in the development of adventitious shoots was evaluated for C. aurantium. Culture medium supplementation with BAP is not essential for the adventitious shoots development in the four genotypes studied when epicotyl segments-derived explants are used. In general, culture media supplementation with BAP decreased the percentage of responsive explants excepted for C. sinensis cv. 'Natal' and C. limonia when the concentrations of 1.5 and 2.0 mg/L were used. The presence of cytokinin, in concentrations up to 2 mg/L, stimulated the in vitro organogenesis when internodal segments-derived explants were used for C. limonia and C. aurantium. For C. aurantium no adventitious shoots developed in explants (internodal segments cultured in basal culture medium, without BAP supplementation. Although no statistic differences could be detected, culture media supplementation with the combination of BAP and NAA favored the development of adventitious shoots in C. aurantium. The best concentration of NAA varied according to BAP concentration. The results presented herein, show that Citrus in vitro organogenesis depends on the interaction of culture medium composition, explant differentiation level, and genotype.

  6. Influence of Limonia acidissima L. against the biofilm forming Aeromonas hydrophila isolated from fresh water fishes

    Directory of Open Access Journals (Sweden)

    Srinivasan Ponnuraj

    2015-04-01

    Full Text Available Limonia acidissima is a medicinal plant commonly used for multitude of ailments. In this context, the validation of traditionally used medicinal plant that the fruit extracts of L. acidissima exhibit antibiofilm activity against the predominant fish pathogen Aeromonas hydrophila was assessed. Among ten isolates of Aeromonas spp, KUAH1 showed strong biofilm formation and was studied further. The methanol (LA-M and ethyl acetate (LA-EA fraction of Limonia fruit extract clearly demonstrated significant (p ≤ 0.005 antibiofilm activity of 58 to 94% and 54 to 77% respectively. Furthermore, the potential of Limonia fruit extracts against some of the biofilm associated factors were tested by swimming and swarming motility assay, XTT and anti-haemolytic activity assay. Extracellular protein analysis revealed differential protein expression at molecular weight corresponding to 30-60 kDa. This is the first report on antibiofilm activity of L. acidissima fruit extracts, signifying the scope for development of complementary medicine to treat Aeromonas biofilm-associated infections.

  7. An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis

    OpenAIRE

    Adriano R. Lucheta; Ana Carla O. Silva-Pinhati; Ana Carolina Basílio-Palmieri; Irving J. Berger; Juliana Freitas-Astúa; Mariângela Cristofani

    2007-01-01

    Citrus species are known by their high content of phenolic compounds, including a wide range of flavonoids. In plants, these compounds are involved in protection against biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. In humans, flavonoid consumption has been related to increasing overall health and fighting some important diseases. The goals of this study were to identify expressed sequence tags (EST) in Citrus sinensis (L.) Osbeck co...

  8. The effects of the use of organic solid wastes on the growth of citrus trees

    OpenAIRE

    Costa, M; Beltrão, J.; Brito, J. C.; Neves, Alcinda; Correia, Maribela Pestana; Guerrero, C.

    2010-01-01

    Two types of organic solid wastes were selected to be applied in a citrus orchard: the sewage sludge (the most common on the settlements) and the manure (the most common on the agricultural fields). The sewage sludge is a residue originated from the wastewater treatment - the solid phase. Its application as an organic fertilizer may represent an alternative to the pollution effects in nature. In an orchard of orange-trees (Citrus sinensis [L.] Osbeck), the application of sludge was compared w...

  9. Occurrence, molecular characterisation, and pathogenicity of Neoscytalidium dimidiatum on Citrus in Italy

    OpenAIRE

    Polizzi, G.; Aiello, D.; Castello, I.; Vitale, A.; Groenewald, J.Z.; Crous, P.W.; A. Gentile; LA MALFA, S

    2011-01-01

    During 2008 and 2009, a new disease was noticed in eastern Sicily (Italy) in two re-grafted citrus orchards (Citrus sinensis (L.) Osbeck) on sour orange rootstock. Symptoms consisted of blight of vigorously growing shoots and a sooty canker on shoots and rootstock trunks. A Scytalidium-like fungus was isolated consistently from symptomatic tissues on 2% potato-dextrose agar (PDA). The mycelium was composed of branched, septate, brown hyphae which disarticulated into 0-1-septate phragmospores....

  10. Occurrence, molecular characterisation, and pathogenicity of Neoscytalidium dimidiatum on citrus in Italy

    OpenAIRE

    Polizzi, G.; Aiello, D.; Castello, I.; Vitale, A.; Groenewald, J.Z.; Crous, P.W.

    2011-01-01

    During 2008 and 2009, a new disease was noticed in eastern Sicily (Italy) in two re-grafted citrus orchards (Citrus sinensis (L.) Osbeck) on sour orange rootstock. Symptoms consisted of blight of vigorously growing shoots and a sooty canker on shoots and rootstock trunks. A Scytalidium-like fungus was isolated consistently from symptomatic tissues on 2% potato-dextrose agar (PDA). The mycelium was composed of branched, septate, brown hyphae which disarticulated into 0-1-septate phragmospores....

  11. Embryogenic calli induction from nucellar tissue of Citrus cultivars Indução de calos embriogênicos a partir de nucelos de variedades de Citrus

    Directory of Open Access Journals (Sweden)

    Fernanda Januzzi Mendes-da-Glória

    1999-01-01

    Full Text Available Nucellar tissues of seven Citrus varieties were introduced onto three growth media to produce embryogenic callus. The media tested were: EME [MT, modified, with the addition of malt extract (500 mg.L-1]; 1/2-EME [half concentration of MT macronutrients + half concentration of BH3 macronutrients + 500 mg.L-1 malt extract + 1.55 g.L-1 of glutamine]; and EBA [EME + 0.44 muM 6-benzyladenine + 0.04 muM 2,4 D]. Soft friable calli were obtained from 'Cravo' and 'Ponkan' mandarins (Citrus reticulata, Blanco, 'Murcott' tangor (Citrus reticulata Blanco x Citrus sinensis L. Osbeck, 'Serra d'água' and 'Valencia' sweet oranges (Citrus sinensis, L. Osbeck 120 days after callus induction. 'Natal' and 'Pera' sweet oranges (Citrus sinensis, L. Osbeck produced hard non-friable calli in this period. EME and 1/2-EME media had the best results for 'Cravo' mandarin, 'Ponkan' mandarin and 'Serra d'água' sweet orange, whereas EBA was the best media composition to induce soft friable calli on 'Murcott' tangor and 'Valencia' sweet orange. Friable callus cultures of 'Cravo' and 'Ponkan' mandarins, and 'Murcott' tangor yielded high quality protoplasts after isolation. Abbreviations: a.c. - activated charcoal; BA - 6-benzyladenine; IAA - indole-acetic acid; 2,4-D - 2,4-diclorophenoxyacetic acid; MT - Murashige & Tucker basal medium.Nucelos de sete variedades de Citrus foram introduzidos em três meios de cultura para produção de calos embriogênicos. Os meios de cultura testados foram: EME [MT, modificado pela adição de extrato de malte (500 mg.L-1]; 1/2-EME [1/2 concentração de macronutrientes no meio MT + 1/2 concentração de macronutrientes no meio BH3 + 500 mg.L-1 extrato de malte + 1,55 g.L-1 de glutamina]; e EBA [EME + 0,44 miM 6-benziladenina + 0,04 miM 2,4 D]. Calos friáveis foram obtidos nas variedades tangerinas 'Cravo' e 'Ponkan' (Citrus reticulata, Blanco, tangor 'Murcote' (Citrus reticulata Blanco x Citrus sinensis L. Osbeck, laranja 'Valencia

  12. Regional Division of Production and Development Strategy of Citrus in Hunan Province

    Institute of Scientific and Technical Information of China (English)

    Yu YANG; Wen DENG; Jianquan LI; Weihong WANG; Guolin HUANG; Ping ZHANG

    2012-01-01

    [Objective] The aim was to research regional division of citrus production and development strategies in Hunan Province. [Method] According to Rural Statisti- cal Yearbook in Hunan Province during 2002-2011, Citrus in Hunan province were divided into three production regions, namely, superior region, sub-superior and non- superior region. On the base of the divisions, the ecological regionalization and brand strategy, Optimization of regional distribution and developing strategy were proposed, with consideration of avoiding frozen zones, in this paper. [Result] Fresh and processing bases of mandarin orange (C.unshiu Marc), and specialty industries of seedless ponkan(C.reticulata Blanco), Bingtang orange (C.sinensis Osbeck Bing- tangcheng), Dayongjuhuaxinyou (Cgrandis (L.) Osbeck Dayongjuhuaxinyou) and An- jiangxiangyou (C.grandis (L.) Osbeck Anjiangxiangyou) should be constructed, where fresh fruit is dominant, supplemented by canned fruit and juice. Industry belt of sat- suma orange, fresh or processing food, is mainly built, for proportion of early and earlier ripe satsuma orange is over 50% of total yield in Xiangzhong citrus zone; in- dustry belt of excellent fresh navel orange and processing sweet orange should be highlighted in Xiangnan. [Conclusion] The research provides references for decision- making for governments, especially on optimization of citrus production regions and development of citrus industry.

  13. Differences between elemental composition of orange juices and leaves from organic and conventional production systems

    International Nuclear Information System (INIS)

    A comprehensive sampling of organic and conventional oranges was carried out in Bebedouro, an important citrus producing region of Brazil. The soils, leaves and fruits of the variety Valencia (Citrus sinensis [L.] Osbeck) budded on Rangpur lime (Citrus limonia Osbeck) were analyzed. The chemical characterization was accomplished by instrumental neutron activation analysis (INAA). Indications for a difference between organic and conventional orange juices and leaves were obtained by applying univariate and multivariate statistical analysis. There were differences between samples for Br, Co, Cs, La and Rb from both systems. (author)

  14. Efficacy ofLimonia acidissima L. (Rutaceae) leaf extract on larval immatures of Culex quinquefasciatus Say 1823

    Institute of Scientific and Technical Information of China (English)

    Siddharthasankar Banerjee; Someshwar Singha; Subrata Laskar; Goutam Chandra

    2011-01-01

    Objective:To investigate the role of leaf extract ofLimonia acidissimaL. (Rutaceae) as a biocontrol agent against the larval form ofCulex quinquefasciatus, and characterization of bioactive component responsible for larvicidal activity.Methods:Larval mortality of mosquito species was observed after24, 48and72 hours of exposure to different concentrations of aqueous extract, solvent extract and subsequently bioactive compound. The bioactive compound was subjected toIR andGC-MS analysis.Results:Mortality rate at 3% concentration of crude extract were highest(90%) amongst all concentrations tested and subsequently highest(95%) mortality was achieved in chloroform: methanol extract at100 ppm concentrations. IRandGC-MS analysis of bioactive compound revealed the presence of steroid compound which may act as larvicide. Conclusions:The chloroform: methanol extract of mature leaves of Limonia acidissima was found to exhibit considerable mosquito larvicidal activity against Culex quinquefasciatus.

  15. Effect of drought stress on mineral nutrient contents in leaves of sdeedling plant of C. tangerina Hort., C. sinensis Osbeck and C. grandis Osbeck%干旱胁迫对实生红橘、甜橙和柚叶中营养元素含量的影响

    Institute of Scientific and Technical Information of China (English)

    马文涛; 樊卫国

    2007-01-01

    以红橘(Citrus tangerina Hort.)、甜橙(Citrus sinensis Osbeck)、柚(Citrus grandis Osbeck)实生苗为材料,采用模拟降雨控水长期干旱胁迫的方法,研究了降水量400和800 mm的干旱胁迫对3种柑橘实生苗叶片的N、P、K、Ca、Mg、Fe、Zn、Cu、Mn、B元素含量的影响.结果表明:干旱胁迫降低了实生甜橙、红橘和柚的叶片中N、P、K、Ca、Mg、Fe、Cu、B元素的含量,增加了叶中Mn元素的含量;实生红橘和柚叶中的Zn含量在降水量400 mm的干旱胁迫下显著降低,而同样的干旱胁迫条件下,实生甜橙叶中的Zn含量极显著地增加了.干旱胁迫降低实生甜橙和红橘叶中N、Ca、Mg、Fe、Cu、B元素含量的作用相对较大.

  16. Citrus tissue culture employing vegetative explants.

    Science.gov (United States)

    Chaturvedi, H C; Singh, S K; Sharma, A K; Agnihotri, S

    2001-11-01

    Citrus being a number one fruit of the world due to its high nutritional value, huge production of fruits and fruit products, the citrus industry may be considered a major fruit industry. Though citrus orchard area in India is comparable to USA, the produce is far less, while its export is nil. Biotechnology has played an outstanding role in boosting the citrus industry, e.g., in Spain, which is now the biggest exporter of citrus fruit with the application of micrografting. Amongst the fruit trees, perhaps the maximum tissue culture research has been done in citrus during the past four decades, however, the results of practical value are meagre. The shortfalls in citrus tissue culture research and some advancements made in this direction along with bright prospects are highlighted, restricting the review to vegetative explants only. Whilst utilization of nucellar embryogenesis is limited to rootstocks, the other aspects, like, regeneration and proliferation of shoot meristems measuring 200 microm in length--a global breakthrough--of two commercially important scion species, Citrus aurantifolia and C. sinensis and an important rootstock, C. limonia, improvement of micrografting technique, cloning of the same two scion species as well as some Indian rootstock species, employing nodal stem segments of mature trees, of immense practical value have been elaborated. A rare phenomenon of shift in the morphogenetic pattern of differentiation from shoot bud differentiation to embryoid formation occurred during the long-term culture of stem callus of C. grandis. Stem callus-regenerated plants of C. aurantifolia, C. sinensis and C. grandis showed variation in their ploidy levels and a somaclonal variant of C. sinensis, which produced seedless fruits was isolated. Tailoring of rooting in microshoots to a tap root-like system by changing the inorganic salt composition of the rooting medium, resulting in 100% transplant success, and germplasm preservation through normal growth

  17. Selection processes in a citrus hybrid population using RAPD markers

    Directory of Open Access Journals (Sweden)

    Oliveira Roberto Pedroso de

    2003-01-01

    Full Text Available The objective of this work was to evaluate the processes of selection in a citrus hybrid population using segregation analysis of RAPD markers. The segregation of 123 RAPD markers between 'Cravo' mandarin (Citrus reticulata Blanco and 'Pêra' sweet orange (C. sinensis (L. Osbeck was analysed in a F1 progeny of 94 hybrids. Genetic composition, diversity, heterozygosity, differences in chromosomal structure and the presence of deleterious recessive genes are discussed based on the segregation ratios obtained. A high percentage of markers had a skeweness of the 1:1 expected segregation ratio in the F1 population. Many markers showed a 3:1 segregation ratio in both varieties and 1:3 in 'Pêra' sweet orange, probably due to directional selection processes. The distribution analysis of the frequencies of the segregant markers in a hybrid population is a simple method which allows a better understanding of the genetics of citrus group.

  18. In vitro pollen germination of five citrus species

    International Nuclear Information System (INIS)

    The aim of present study is In vitro germination of the pollen grains of five Citrus species belonging to the family Rutaceae viz., Citrus aurantium L. var., aurantium Hook.f., C. limon (L.) Brum. f., C. paradisii Macfad, C. reticulata Blanco and C. sinensis (L.) Osbeck. using hanging drop technique. The germination was checked up to 48 weeks, for the pollen stored at different temperatures like 4 degree C, -20 degree C, -30 degree C and -60 degree C. The study indicates that low temperature and low relative humidity is better than high temperature and humidity with respect to pollen germination capacity and viability. Freeze dryer (-60 degree C) seems to be the best method to maintain pollen viability of stored pollen grains for a long period of time. Among five species Citrus aurantium, C. limon and C. sinensis showed high percentage of germination as compared to C. reticulata and C. paradisii. (author)

  19. Carbohydrate levels in the leaves and production consistency of the Ponkan tangerine when thinned out with Ethephon Teores de carboidratos nas folhas e regularidade da produção de tangerineira 'Ponkan' raleadas com Ethephon

    OpenAIRE

    Rodrigo Amato Moreira; José Darlan Ramos; Maria do Céu Monteiro da Cruz; Lílian Araújo Pantoja; Alexandre Soares dos Santos

    2013-01-01

    Management practices that maintain carbohydrate reserves in tangerines are necessary in order to ensure good yields. This experiment was carried out with the object of evaluating the levels of carbohydrates in the leaves, and consistency in the production of the Ponkan tangerine (Citrus reticulata Blanco) when subjected to chemical thinning with five concentrations of Ethephon for three consecutive years. The Ponkan tangerine evaluated had been grafted onto Rangpur lime (Citrus limonia Osbeck...

  20. Variação de matéria seca e de nutrientes nas folhas e nos frutos, produção de ácido ascórbico e suco, em seis cultivares de citros, durante um ciclo Six citrus cultivars comparatively evaluated as to their fruit and leaf dry weights and nutrient concentrations

    Directory of Open Access Journals (Sweden)

    H.P. Haag

    1993-09-01

    Full Text Available De uma plantação de citros, com os cultivares T. Cravo (Citrus reticulata Blanco, L.Hamlin (Citrus sinensis (L. Osbeck, T. Murcott (Citrus reticulata Blanco x Citrus sinensis (L. Osbeck, L. Natal (Citrus sinensis (L. Osbeck, L. Valencia (Citrus sinensis (L. Osbeck e L. Pera (Citrus sinensis (L. Osbeck, situada na "Fazenda Sete Lagoas", no município de Mogi-Guaçu (22° 22% 46° 56'W.Gr., em Latossolo Vermelho amarelo, fase arenosa, foram coletados frutos 30 dias após florescimento, até a idade da coleta comercial. No material coletado, foram determinadas a variação da matéria seca, a concentração dos macro e micronutrientes nas folhas adjacentes ao fruto, a extração de macro e micronutríentes pelos frutos, a produção de suco (ml por fruto e a concentração de ácido ascórbico (mg/100 ml de suco. Concluiu-se que: 1. O aumento da matéria seca, intensifica-se a partir do segundo mês apos o florescimento; 2. Com exceção da T. Cravo, ocorre uma diminuição na produção de matéria seca no final do ciclo; 3. A concentração dos macro e micronutrientes nas folhas apresenta oscilações durante o desenvolvimento do fruto; 4. A ordem decrescente de extração de nutrientes é: K, N, Ca, Mg, P = S, Fe, B, Zn, Mn, Cu; 5. A capacidade de exportação de nutrientes pelos cultivares é, em ordem decrescente: L. Pera, L. Hamlin = T. Cravo, T. Murcott, L. Valencia, L. Natal; 6. A quantidade de suco produzido por fruto, oscila entre 43 a 95 ml; 7. A concentração de ácido ascórbico (mg/100 ml de suco, varia entre 30 a 95.The experiment was carried out in a commercial citrus orchard located in Mogi-Guaçu (22° 22'S., 46° 56'WGr., State of São Paulo, Brazil. Five orange types were studied: Cravo (Citrus reticulata Blanco; Hamlin (Citrus sinensis (L. Osbeck; Natal (Citrus sinensis (L. Osbeck; Valencia (Citrus sinensis (L. Osbeck and Pera (Citrus sinensis (L. Osbeck and a tangerine Murcott (Citrus reticulata Blanco x Citrus sinensis (L

  1. Host susceptibility of citrus cultivars to Queensland fruit fly (Diptera: Tephritidae).

    Science.gov (United States)

    Lloyd, A C; Hamacek, E L; Smith, D; Kopittke, R A; Gu, H

    2013-04-01

    Citrus crops are considered to be relatively poor hosts for Queensland fruit fly, Bactrocera tryoni (Froggatt), as for other tephritid species. Australian citrus growers and crop consultants have reported observable differences in susceptibility of different citrus cultivars under commercial growing conditions. In this study we conducted laboratory tests and field surveys to determine susceptibility to B. tryoni of six citrus cultivars [(Eureka lemon (Citrus limon (L.) Osbeck); Navel and Valencia oranges (C. sinensis (L.) Osbeck); and Imperial, Ellendale, and Murcott mandarins (C. reticulata Blanco). The host susceptibility of these citrus cultivars was quantified by a Host Susceptibility Index, which is defined as the number of adult flies produced per gram of fruit infested at a calculated rate of one egg per gram of fruit. The HSI was ranked as Murcott (0.083) > Imperial (0.052) > Navel (0.026) - Ellendale (0.020) > Valencia (0.008) > Eureka (yellow) (0.002) > Eureka (green) (0). Results of the laboratory study were in agreement with the level of field infestation in the four citrus cultivars (Eureka lemon, Imperial, Ellendale, and Murcott mandarins) that were surveyed from commercial orchards under baiting treatments against fruit flies in the Central Burnett district of Queensland. Field surveys of citrus hosts from the habitats not subject to fruit fly management showed that the numbers of fruit flies produced per gram of fruit were much lower, compared with the more susceptible noncitrus hosts, such as guava (Psidium guajava L.), cherry guava (P. littorale Raddi), mulberry (Morus nigra L.), loquat (Eriobotrya japonica (Thunb.) Lindl.), and pear (Pyrus communis L.). Therefore, the major citrus crops commercially cultivated in Australia have a relatively low susceptibility to B. tryoni, with Eureka lemons being a particularly poor host for this tephritid fruit fly. PMID:23786078

  2. Cellulose nanocrystal from pomelo (C. Grandis osbeck) albedo: Chemical, morphology and crystallinity evaluation

    International Nuclear Information System (INIS)

    Citrus peel is one of the under-utilized waste materials that have potential in producing a valuable fibre, which are cellulose and cellulose nanocrystal. Cellulose was first isolated from pomelo (C. Grandis Osbeck) albedo by combination of alkali treatment and bleaching process, followed by acid hydrolysis (65% H2SO4, 45 °C, 45min) to produce cellulose nanocrystal. The crystalline, structural, morphological and chemical properties of both materials were studied. Result reveals the crystallinity index obtained from X-ray diffraction for cellulose nanocrystal was found higher than extracted cellulose with the value of 60.27% and 57.47%, respectively. Fourier transform infrared showed that the chemical treatments removed most of the hemicellulose and lignin from the pomelo albedo fibre. This has been confirmed further by SEM and TEM for their morphological studies. These results showed that cellulose and cellulose nanocrystal were successfully obtained from pomelo albedo and might be potentially used in producing functional fibres for food application

  3. Cellulose nanocrystal from pomelo (C. Grandis osbeck) albedo: Chemical, morphology and crystallinity evaluation

    Science.gov (United States)

    Zain, Nor Fazelin Mat; Yusop, Salma Mohamad; Ahmad, Ishak

    2013-11-01

    Citrus peel is one of the under-utilized waste materials that have potential in producing a valuable fibre, which are cellulose and cellulose nanocrystal. Cellulose was first isolated from pomelo (C. Grandis Osbeck) albedo by combination of alkali treatment and bleaching process, followed by acid hydrolysis (65% H2SO4, 45 °C, 45min) to produce cellulose nanocrystal. The crystalline, structural, morphological and chemical properties of both materials were studied. Result reveals the crystallinity index obtained from X-ray diffraction for cellulose nanocrystal was found higher than extracted cellulose with the value of 60.27% and 57.47%, respectively. Fourier transform infrared showed that the chemical treatments removed most of the hemicellulose and lignin from the pomelo albedo fibre. This has been confirmed further by SEM and TEM for their morphological studies. These results showed that cellulose and cellulose nanocrystal were successfully obtained from pomelo albedo and might be potentially used in producing functional fibres for food application.

  4. Cellulose nanocrystal from pomelo (C. Grandis osbeck) albedo: Chemical, morphology and crystallinity evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Zain, Nor Fazelin Mat; Yusop, Salma Mohamad [Food Science Program, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor (Malaysia); Ahmad, Ishak [Polymer Research Centre (PORCE), School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor (Malaysia)

    2013-11-27

    Citrus peel is one of the under-utilized waste materials that have potential in producing a valuable fibre, which are cellulose and cellulose nanocrystal. Cellulose was first isolated from pomelo (C. Grandis Osbeck) albedo by combination of alkali treatment and bleaching process, followed by acid hydrolysis (65% H{sub 2}SO{sub 4}, 45 °C, 45min) to produce cellulose nanocrystal. The crystalline, structural, morphological and chemical properties of both materials were studied. Result reveals the crystallinity index obtained from X-ray diffraction for cellulose nanocrystal was found higher than extracted cellulose with the value of 60.27% and 57.47%, respectively. Fourier transform infrared showed that the chemical treatments removed most of the hemicellulose and lignin from the pomelo albedo fibre. This has been confirmed further by SEM and TEM for their morphological studies. These results showed that cellulose and cellulose nanocrystal were successfully obtained from pomelo albedo and might be potentially used in producing functional fibres for food application.

  5. Cloning and analysis of LFY-like gene from Citrus%柑橘LEAFY同源基因片段的克隆及分析

    Institute of Scientific and Technical Information of China (English)

    刘淑芳; 陈力耕; 陈大明; 徐昌杰

    2001-01-01

    在提取大三岛脐橙(Citrus sinensis (L) Osbeck)基因组DNA的基础上,利用Uneven PCR的方法克隆了脐橙中的LEAFY(LFY)同源基因片段。经对该基因片段的核苷酸序列分析表明,它和烟草中的LFY同源基因NFY基因在结构上高度保守,同源性高达90%以上。%The homologous DNA fragment of LFY was cloned from citrus genomic DNA that had been isolated from Sweet orange (Citrus sinensis (L) Osbeck)using Uneven PCR. Analysis of the nucleotide sequence indicated that homology was up to 90% between it and NFY, another LFY-like gene, which implied that they were highly conserved on their structure.

  6. Analysis of Major Carotenoid Composition and Its Content of Citrus Fruit

    Institute of Scientific and Technical Information of China (English)

    TAO Jun; ZHANG Shang-long; ZHANG Liang-cheng; XU Jian-guo; LIU Chun-rong

    2003-01-01

    a-carotene, β-carotene, lycopene, β-cryptoxanthin, zeaxanthin and lutein content of fruits in53 citrus cultivars were determined using HPLC. In both peel and pulp of citrus fruit, the major carotenoidswere lutein, zeaxanthin and β-cryptoxanthin. β-carotene content was relatively low and extremely low was theamount of or-carotene. Among the 53 cultivars tested, lycopene was detected only in pulp of Cara Cara navelorange. Carotenoid content in both peel and pulp of citrus fruit was the highest in Citrus reticulata Blanco andlowest in Citrus grandis Osbeck. Consequently, as far as the health protection value is considered, fruit ofCitrus reticulata Blanco ranks probably higher than other citrus fruits. In fruit of most Citrus retieulatavarities, β-cryptoxanthin was the main carotenoid component in pulp and its amount approximated that of lu-tein in peel. Content of lutein, zeaxanthin and β-cryptoxanthin in peel was about 2.5 - 15 times that in pulp onthe basis of fresh weight. Thus peel was inferred to be the principal location for the carotenoid stock in citrus fruit.

  7. Rizobactérias e promoção do crescimento de plantas cítricas Rhizobacteria and growth promotion of citrus plants

    Directory of Open Access Journals (Sweden)

    S. S. Freitas

    2004-12-01

    Full Text Available Desenvolveram-se três experimentos em casa de vegetação para verificar a possibilidade de as rizobactérias atuarem como promotoras do crescimento de plantas cítricas. Ao todo, testaram-se 10 isolados de Pseudomonas do grupo fluorescente, 13 de Bacillus e sete de outras bactérias rizosféricas em porta-enxertos utilizados na citricultura: tangerineira 'Cleópatra' (Citrus reshni, limoeiro 'Cravo' (Citrus limonia e limoeiro 'Volcameriano' (Citrus volkameriana. Dependendo do porta-enxerto, sete isolados de Pseudomonas, um de Bacillus e um de outra bactéria rizosférica tiveram efeito benéfico sobre a matéria seca de raízes ou de parte aérea, indicando uma alta proporção de promotores de crescimento entre as bactérias do primeiro grupo. Procedeu-se também à contagem de bactérias fluorescentes do gênero Pseudomonas e de bactérias não-fluorescentes em raízes de tangerineira 'Cleópatra' e de limoeiro 'Cravo', procedentes de viveiro de mudas e do campo. Ambos os grupos bacterianos tiveram sua multiplicação favorecida na rizosfera de tangerineira 'Cleópatra', em condições de viveiro.Three greenhouse trials were carried out to verify if rhizobacteria can promote citrus plant growth. Ten isolates of fluorescent Pseudomonads, thirteen of Bacillus spp. and seven of other rhizospheric bacteria were tested in three rootstocks seedlings: 'Cleopatra' mandarin (Citrus reshni, rangpure lime (Citrus limonia and Volkamerian lemon (Citrus volkameriana. Depending on the rootstock, seven Pseudomonads, one isolate of Bacillus and one of other rhizospheric bacteria increased the root or shoot dry weight, indicating a high proportion of growth promoters among the fluorescent Pseudomonads. Also, fluorescent Pseudomonads and non fluorescent bacteria were counted in the roots of nursery seedlings and field plants of Citrus reshni and Citrus limonia. The growth of both bacterial groups was favored in the Citrus reshni rhizosphere under nursery

  8. Citrus Genomics

    OpenAIRE

    Talon, Manuel; Gmitter, Fred G.Jr.

    2008-01-01

    Citrus is one of the most widespread fruit crops globally, with great economic and health value. It is among the most difficult plants to improve through traditional breeding approaches. Currently, there is risk of devastation by diseases threatening to limit production and future availability to the human population. As technologies rapidly advance in genomic science, they are quickly adapted to address the biological challenges of the citrus plant system and the world's industries. The hist...

  9. Phylogenetic relationships of citrus and its relatives based on matK gene sequences.

    Directory of Open Access Journals (Sweden)

    Tshering Penjor

    Full Text Available The genus Citrus includes mandarin, orange, lemon, grapefruit and lime, which have high economic and nutritional value. The family Rutaceae can be divided into 7 subfamilies, including Aurantioideae. The genus Citrus belongs to the subfamily Aurantioideae. In this study, we sequenced the chloroplast matK genes of 135 accessions from 22 genera of Aurantioideae and analyzed them phylogenetically. Our study includes many accessions that have not been examined in other studies. The subfamily Aurantioideae has been classified into 2 tribes, Clauseneae and Citreae, and our current molecular analysis clearly discriminate Citreae from Clauseneae by using only 1 chloroplast DNA sequence. Our study confirms previous observations on the molecular phylogeny of Aurantioideae in many aspects. However, we have provided novel information on these genetic relationships. For example, inconsistent with the previous observation, and consistent with our preliminary study using the chloroplast rbcL genes, our analysis showed that Feroniella oblata is not nested in Citrus species and is closely related with Feronia limonia. Furthermore, we have shown that Murraya paniculata is similar to Merrillia caloxylon and is dissimilar to Murraya koenigii. We found that "true citrus fruit trees" could be divided into 2 subclusters. One subcluster included Citrus, Fortunella, and Poncirus, while the other cluster included Microcitrus and Eremocitrus. Compared to previous studies, our current study is the most extensive phylogenetic study of Citrus species since it includes 93 accessions. The results indicate that Citrus species can be classified into 3 clusters: a citron cluster, a pummelo cluster, and a mandarin cluster. Although most mandarin accessions belonged to the mandarin cluster, we found some exceptions. We also obtained the information on the genetic background of various species of acid citrus grown in Japan. Because the genus Citrus contains many important accessions

  10. 广东曲江区柑橘园天敌瓢虫种类调查及食性观察(鞘翅目:瓢虫科)%Survey and preys of lady beetles (Coccinellidae) in citrus groves in Qujiang, Guangdong

    Institute of Scientific and Technical Information of China (English)

    郑朝武; 虞国跃

    2013-01-01

    对广东曲江区4个柑橘园4种橘类植物(甜橙Citrus sinensis(Linn.)Osbeck,沙田柚Citrus grandis(Linn.)Osbeck var.shatinyu Hort,温州蜜橘Citrus unshiu Marcovitch和沙糖桔Citrus reticulata Blanco cv.Shiyue Ju)上的瓢虫进行种类调查和食性观察,共采集320头瓢虫标本,鉴定结果为24属51种,多数为蚜虫的捕食性天敌,其中龟纹瓢虫、六斑月瓢虫、红肩瓢虫、黄斑盘瓢虫、台湾隐势瓢虫、后斑小瓢虫和细缘唇瓢虫为优势种.对大多数种类的食性作了观察或进行室内饲养,基本明确了它们在橘类上的猎物.%Survey and preys of lady beetle species in citrus groves were carried out in Qujiang District, Shaoguan City, Guangdong Province between January 2010 to July 2012. The surveyed 4 Citrus plants are Citrus sinensis (Linn. ) Osbeck, C. grandis (Linn. ) Osbeck var. shatinyu Hort, C. unshiu Marcovitch and C. reticulata Blanco cv. Shiyue Ju. A total of 320 lady beetle specimens (excluding plant -eating ones) were collected and identified. They belonged to 51 species of 24 genera. Most of them are predator of citrus aphids, and Propylea japonica (Thunberg, 1781), Cheilomems sexmaculata (Fabricius, 1781), Harmonia dimidiata ( Fabricius, 1755), Lemnia saucia ( Mulsant, 1850), Cryptogonus horishanus (Ohta, 1929), Scymnus (Pullus) posticalis Sicard, 1912 and Chilocorus circumdatus (Gyhhenhal, 1808) were dominant species. Most of them are provided with the observed preys in field or the feeding preys indoor.

  11. Phenols in citrus peel byproducts. Concentrations of hydroxycinnamates and polymethoxylated flavones in citrus peel molasses.

    Science.gov (United States)

    Manthey, J A; Grohmann, K

    2001-07-01

    In addition to the main flavanone glycosides (i.e., hesperidin and naringin) in citrus peel, polymethoxylated flavones and numerous hydroxycinnamates also occur and are major phenolic constituents of the molasses byproduct generated from fruit processing. Although a small number of the hydroxycinnamates in citrus occur as amides, most occur as esters and are susceptible to alkaline hydrolysis. This susceptibility to alkaline hydrolysis was used in measuring the concentrations of hydroxycinnamates in citrus peel molasses. The highest concentrations of hydroxycinnamates occurred in molasses of orange [C. sinensis (L.) Osbeck] and tangerine (C. reticulata Blanco.) compared to grapefruit (C. paradisi Macf.) and lemon [C. limon (L.) Burm.]. Concentrations of two phenolic glucosides, phlorin (phloroglucinol-beta-O-glucoside) and coniferin (coniferyl alcohol-4-beta-O-glucoside), were also measured. Measurements of the polymethoxylated flavones in molasses from several tangerine and orange varieties showed that these compounds occurred in the highest amounts in Dancy tangerine, whereas samples from two other tangerine molasses contained significantly lower levels, similar to those in the molasses samples from late- and early/mid-season oranges. PMID:11453761

  12. Structure and composition of the assemblage of parasitoids associated to Phyllocnistis citrella pupae Stainton (Lepidoptera: Gracillariidae) in citrus orchards in Southern Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Jahnke, Simone M.; Redaelli, Luiza R.; Soglio, Fabio K. Dal [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Dept. de Fitossanidade; Diefenbach, Lucia M.G. [Fundacao Estadual de Producao e Pesquisa em Saude (FEPPS), Porto Alegre, RS (Brazil). Inst. de Pesquisas Biologicas. Lab. Central do Estado (LACEN)

    2007-09-15

    The structure and composition of the assemblage of pupal parasitoids of Phyllocnistis citrella Stainton, the citrus leaf miner, were studied in two citrus orchards (Citrus deliciosa Tenore cv. Montenegrina and Citrus sinensis (L.) Osbeck x Citrus reticulata Blanco hybrid Murcott), in Montenegro County (29 deg 68S and 51 deg 46W), southern Brazil. At fortnightly samplings, from July 2001 to June 2003, all the new shoots from 24 randomly selected trees were inspected. The species richness reached five native species in the Murcott orchard, and six in Montenegrina. In Murcott, the presence of Ageniaspis citricola (Hymenoptera: Encyrtidae), an exotic species, was detected in the first year of sampling, probably migrating from the nearby areas where it had been released for the miner control. In Montenegrina, its presence was only registered in the second year. A. citricola in both areas was dominant and changed the community structure of parasitoid complex of P. citrella in both orchards. (author)

  13. Chemical constituents and larvicidal potential of Feronia limonia leaf essential oil against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus.

    Science.gov (United States)

    Senthilkumar, A; Jayaraman, M; Venkatesalu, V

    2013-03-01

    In the present investigation, the leaf essential oil of Feronia limonia was evaluated for chemical constituents and mosquito larvicidal activity against the larvae of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. GC and GC-MS analyses revealed that the essential oil contain 51 compounds. Estragole (34.69 %) and β-pinene(23.59 %) were identified as the major constituents followed by methyl (Z)-caryophyllene (11.05 %), eugenol (6.50 %), linalool (3.97 %), phytol (3.27 %), sabinene (2.41 %) and limonene (2.27 %). Larval mortality was observed after 12 and 24 h of exposure period. The oil showed remarkable larvicidal activity against A. stephensi (LC(50) = 38.93 and LC(90) = 108.64 ppm (after 12 h); LC(50) = 15.03 and LC(90) = 36.69 ppm (after 24 h)), A. aegypti (LC(50) = 37.60 and LC(90) = 104.69 ppm (after 12 h); LC(50) = 11.59 and LC(90) = 42.95 ppm (after 24 h)) and C. quinquefasciatus (LC(50) = 52.08 and LC(90) = 124.33 ppm (after 12 h); LC(50) = 22.49 and LC(90) = 60.90 ppm (after 24 h)). Based on the results, the essential oil of F. limonia can be considered as a new source of larvicide for the control of vector mosquitoes.

  14. An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis

    Directory of Open Access Journals (Sweden)

    Adriano R. Lucheta

    2007-01-01

    Full Text Available Citrus species are known by their high content of phenolic compounds, including a wide range of flavonoids. In plants, these compounds are involved in protection against biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. In humans, flavonoid consumption has been related to increasing overall health and fighting some important diseases. The goals of this study were to identify expressed sequence tags (EST in Citrus sinensis (L. Osbeck corresponding to genes involved in general phenylpropanoid biosynthesis and the key genes involved in the main flavonoids pathways (flavanones, flavones, flavonols, leucoanthocyanidins, anthocyanins and isoflavonoids. A thorough analysis of all related putative genes from the Citrus EST (CitEST database revealed several interesting aspects associated to these pathways and brought novel information with promising usefulness for both basic and biotechnological applications.

  15. ESTIMATIVA DA ÁREA FOLIAR DE LIMOEIRO-CRAVO USANDO DIMENSÕES LINEARES DO LIMBO FOLIAR

    OpenAIRE

    Émile Costa Melo; Raimundo Leonardo Lima de Oliveira; Layla Gerusa Souza Lima; Lillian Matias de Oliveira; Ariele Carneiro de Andrade; Raimundo Thiago Lima da Silva; Cândido Ferreira de Oliveira

    2013-01-01

    In order to obtain equations which, through dimensional linear parameters of the leaves, allows the estimation of leaf area of rangpur lime (Citrus limonia (L.) Osbeck) an experiment was conducted in a rural area of Capitão Poço - PA. Leaf area was determined by the method of discs. Linear, linear without intercept, quadratic, cubic, logarithmic, exponential and potential models were adjusted between leaf area and length, the width, the sum (length + width) and its products (length x width), ...

  16. Measurements on hydrocyanic acid absorbed by citrus tissues during fumigation

    Energy Technology Data Exchange (ETDEWEB)

    Bartholomew, E.T.; Sinclair, W.B.; Lindgren, D.L.

    1942-05-01

    Methods for the accurate determination of hydrocyanic acid (HCN) and studies of factors affecting the recovery of HCN from fumigated citrus tissues have been previously reported. This study is concerned with the results of the application of the principles derived from the earlier studies to further laboratory experiments, performed in conjunction with the fumigation studies of citrus trees under orchard conditions. The effect of certain factors, such as oil sprays, the locality in which the trees were grown, and the temperature, age, and moisture content of citrus tissues at time of fumigation, have been studied in relation to the absorption and retention of HCN under both laboratory and field conditions. The comparative amounts of absorption and lengths of time of retention of HCN have also been studied in relation to maturity of leaves and fruits and in relation to their injurious or noninjurious effects. The results of laboratory experiments cannot always be applied directly to the solution of orchard fumigation problems, but they may serve as a basis for the formulation of field experiments. The trees, leaves, and fruits used in the experiments described in this study were of the Valencia-orange variety (Citrus sinensis Osbeck). 13 references, 9 figures, 11 tables.

  17. New excised-leaf assay method to test inoculativity of Asian citrus psyllid (Hemiptera: Psyllidae) with Candidatus Liberibacter asiaticus associated with citrus huanglongbing disease.

    Science.gov (United States)

    Ammar, El-Desouky; Walter, Abigail J; Hall, David G

    2013-02-01

    The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the primary vector of Candidatus Liberibacter asiaticus (Las) associated with huanglongbing, or citrus greening, the most devastating citrus (Citrus spp.) disease worldwide. Here, we developed a new "excised-leaf assay" that can speed up Las-inoculativity tests on Asian citrus psyllid from the current 3-12 mo (when using whole citrus seedlings for inoculation) to only 2-3 wk. Young adults of Asian citrus psyllid that had been reared on Las-infected plants were caged on excised healthy sweet orange [Citrus sinensis (L.) Osbeck] leaves for a 1-2-wk inoculation access periods (IAP), and then both psyllids and leaves were tested later by quantitative polymerase chain reaction (PCR). When single adults were tested per leaf, percentages of Las-positive leaves averaged 2-6% by using HLBaspr primers and 10-20% by using the more sensitive LJ900 primers. Higher proportions of Las-positive leaves were obtained with 1) higher densities of inoculating psyllids (5-10 adults per leaf), 2) longer IAPs, and 3) incubation of leaves for 1 wk postinoculation before PCR. Logistic regression analysis indicated a positive correlation between Las titer in Asian citrus psyllid adults tested singly and the probability of detecting Las in the inoculated leaves, correlations that can be very useful in epidemiological studies. Comparison between excised leaves and whole seedlings, inoculated consecutively for 1 wk each by one or a group of psyllids, indicated no significant difference between Las detection in excised leaves or whole plants. This new excised-leaf assay method saves considerable time, materials, and greenhouse space, and it may enhance vector relation and epidemiological studies on Las and potentially other Liberibacter spp. associated with huanglongbing disease.

  18. Bioactive compounds in blood oranges (Citrus sinensis (L.) Osbeck): Level and intake.

    Science.gov (United States)

    Fallico, Biagio; Ballistreri, Gabriele; Arena, Elena; Brighina, Selina; Rapisarda, Paolo

    2017-01-15

    Both the composition and the intake of antioxidants (anthocyanins, ascorbic acid and hydroxycinnamic acids) were reported for all blood oranges including the single cultivars (Moro, Tarocco and Sanguinello) and industrially produced juices. The mean values of the studied bioactive compounds in the edible part oranges were: 9.6mg/100g of orange edible part for the anthocyanins; 8.1, 0.7, 1.3, 3.8, 2.5mg/100g for total hydroxycinnamic acids, caffeic, sinapic, ferulic and coumaric acids, respectively and 59.1mg/100g for ascorbic acid. The consumption of blood oranges contributes to a daily intake of: 9.4mg/d (up to 55mg/d) of anthocyanins and 58.5mg/d (up to 340mg/d) of vitamin C, respectively. Data suggest that the 50% of consumers, males and females, receive more than the 70% and 90% of EAR value of vitamin C, respectively. The 25% of males and the 40% of females has an intake higher than the EAR. PMID:27542451

  19. Early Evaluation of Compatibility between Commercial Citrus Varieties and Kaffir Lime (Citrus hystrix and Carrizo Citrange (C. sinensis Osb. x P. trifoliata L. Raf. Rootstocks at Mekong Delta, Vietnam

    Directory of Open Access Journals (Sweden)

    Le Thi Khoe

    2015-01-01

    Full Text Available The results of investigation on early evaluation of compatibility between commercial citrus varieties, and Kaffir lime (Citrus hystrix and Carrizo citrange (C. sinensis Osb. x P. trifoliata L. Raf. rootstocks, at Mekong Delta Vietnam, during four successive years from 2010 to 2013, reported that out of the tested combinations of the recent commercial citrus varieties, included Da xanh pumelo (C. grandis Osbeck, seedless Mat orange (C. sinensis L. Osbeck, Sanh orange (C. nobilis Lour. and Duong mandarin  (C. reticulata, budded on Kaffir lime and Carrizo citrange, in the 3rd and 4th  year after planting under the trial fields at Mekong Delta Vietnam, the trees of Sanh orange (C. nobilis Lour. budded on Kaffir lime (Citrus hystrix were supperior with precocity, producing high number of fruits (16.67 fruits per tree , and very good fruit quality of larger fruit size (334.6 g and 11.16 cm, fruit weight and diameter, respectively, higher total soluble solids (12.5 brix, better orange-yellow juice color development, and excellent flavor and aroma of juice, as compared to the typical characteristics; meanwhile these were followed by trees of seedless Mat orange budded on Kaffir lime, which produced the highest number of fruits (47.33 fruits per tree, with little inferior quality of seedy fruit (1-3 seeds per fruit, lower total soluble solids (7.33%, and no good flavor and aroma, as somewhat differed from the typical fruit traits.

  20. Citrus leprosis research update

    Science.gov (United States)

    Citrus leprosis is one of the oldest citrus diseases, but is also one of the most important emerging citrus diseases in South and Central America, and it is apparently spreading northward towards the U.S. Research in our labs and by others has shown that citrus leprosis disease is caused by a compl...

  1. Resistance of Citrus and Related Genera to Diaphorina citri Kuwayama (Hemiptera: Liviidae).

    Science.gov (United States)

    Borgoni, P C; Vendramim, J D; Lourencão, A L; Machado, M A

    2014-10-01

    The present study was developed to evaluate the resistance of the following genotypes of Citrus and related genera to this pest: 'Pera,' 'Natal', and 'Washington Navel' oranges (Citrus sinensis), 'Marsh Seedless' grapefruit (Citrus paradisi), hardy orange 'Rubidoux' (Poncirus trifoliata), kumquat (Fortunella margarita Swingle), citrumelo 'Swingle' (C. paradisi x P. trifoliata), and citrange 'Troyer' (P. trifoliata x C. sinensis). The experiments were performed in greenhouses with plants grafted onto 'Rangpur' lime (Citrus limonia) and placed individually in voile cages. The preference for oviposition in a no-choice test, and the effect of genotype were evaluated. The egg-adult cycle was monitored to determine the effect of genotype on the biology of the insect. Poncirus 'Rubidoux' was the least preferred genotype for oviposition; reduced number of eggs was also found to occur on citrange 'Troyer', and 'Marsh Seedless' was the genotype with the most eggs. No significant variation in the duration of the embryonic period was observed; however, a difference in the viability of eggs was found, with the lowest egg viabilities on 'Swingle.' Kumquat and 'Marsh Seedless' genotypes were correlated with increased durations of the nymphal phase, however, there was no difference in the survival of this phase. Fecundity of females on 'Troyer', 'Swingle', and kumquat was reduced. Considering all of the evaluated parameters, it was concluded that cultivars of sweet orange are the most susceptible genotypes to Diaphorina citri. Regarding oviposition, P. trifoliata 'Rubidoux' showed resistance of the antixenosis type. PMID:27193957

  2. Resistance of Citrus and Related Genera to Diaphorina citri Kuwayama (Hemiptera: Liviidae).

    Science.gov (United States)

    Borgoni, P C; Vendramim, J D; Lourencão, A L; Machado, M A

    2014-10-01

    The present study was developed to evaluate the resistance of the following genotypes of Citrus and related genera to this pest: 'Pera,' 'Natal', and 'Washington Navel' oranges (Citrus sinensis), 'Marsh Seedless' grapefruit (Citrus paradisi), hardy orange 'Rubidoux' (Poncirus trifoliata), kumquat (Fortunella margarita Swingle), citrumelo 'Swingle' (C. paradisi x P. trifoliata), and citrange 'Troyer' (P. trifoliata x C. sinensis). The experiments were performed in greenhouses with plants grafted onto 'Rangpur' lime (Citrus limonia) and placed individually in voile cages. The preference for oviposition in a no-choice test, and the effect of genotype were evaluated. The egg-adult cycle was monitored to determine the effect of genotype on the biology of the insect. Poncirus 'Rubidoux' was the least preferred genotype for oviposition; reduced number of eggs was also found to occur on citrange 'Troyer', and 'Marsh Seedless' was the genotype with the most eggs. No significant variation in the duration of the embryonic period was observed; however, a difference in the viability of eggs was found, with the lowest egg viabilities on 'Swingle.' Kumquat and 'Marsh Seedless' genotypes were correlated with increased durations of the nymphal phase, however, there was no difference in the survival of this phase. Fecundity of females on 'Troyer', 'Swingle', and kumquat was reduced. Considering all of the evaluated parameters, it was concluded that cultivars of sweet orange are the most susceptible genotypes to Diaphorina citri. Regarding oviposition, P. trifoliata 'Rubidoux' showed resistance of the antixenosis type.

  3. Development and reproduction of Panonychus citri (Prostigmata: Tetranychidae) on different species and varieties of citrus plants.

    Science.gov (United States)

    Zanardi, Odimar Zanuzo; Bordini, Gabriela Pavan; Franco, Aline Aparecida; de Morais, Matheus Rovere; Yamamoto, Pedro Takao

    2015-12-01

    The species and varieties of citrus plants that are currently grown can favor the population growth of the citrus red mite Panonychus citri (McGregor) (Prostigmata: Tetranychidae) and alter the pest management programs in citrus groves. In this study we evaluated, in the laboratory, the development and reproduction of P. citri and estimated its life table parameters when reared on four varieties of Citrus sinensis (L.) Osbeck (Valencia, Pera, Natal, and Hamlin), one variety of Citrus reticulata Blanco (Ponkan) and one variety of Citrus limon (L.) Burm. (Sicilian). The incubation period and egg viability were not affected by the host plant. However, the development and survival of the immature stage were significantly lower on Hamlin orange than on Valencia, Pera and Natal oranges, Ponkan mandarin and Sicilian lemon. The fecundity and oviposition period of females were lower on Hamlin orange than on the other hosts. Mites reared on Valencia orange and Sicilian lemon had a higher net reproductive rate (R 0 ), intrinsic growth rate (r) and finite rate of increase (λ), and a shorter interval between generations (T) than on Pera, Natal and Hamlin oranges and Ponkan mandarin. On the other hand, mites reared on Hamlin orange had the lowest R 0 , r and λ and the highest T among the hosts. Based on the results obtained we recommend that for Valencia orange and Sicilian lemon, the mite monitoring programs should be more intense to detect the initial infestation of pest, avoiding the damage in plants and the increase in production costs. PMID:26459376

  4. Development and reproduction of Panonychus citri (Prostigmata: Tetranychidae) on different species and varieties of citrus plants.

    Science.gov (United States)

    Zanardi, Odimar Zanuzo; Bordini, Gabriela Pavan; Franco, Aline Aparecida; de Morais, Matheus Rovere; Yamamoto, Pedro Takao

    2015-12-01

    The species and varieties of citrus plants that are currently grown can favor the population growth of the citrus red mite Panonychus citri (McGregor) (Prostigmata: Tetranychidae) and alter the pest management programs in citrus groves. In this study we evaluated, in the laboratory, the development and reproduction of P. citri and estimated its life table parameters when reared on four varieties of Citrus sinensis (L.) Osbeck (Valencia, Pera, Natal, and Hamlin), one variety of Citrus reticulata Blanco (Ponkan) and one variety of Citrus limon (L.) Burm. (Sicilian). The incubation period and egg viability were not affected by the host plant. However, the development and survival of the immature stage were significantly lower on Hamlin orange than on Valencia, Pera and Natal oranges, Ponkan mandarin and Sicilian lemon. The fecundity and oviposition period of females were lower on Hamlin orange than on the other hosts. Mites reared on Valencia orange and Sicilian lemon had a higher net reproductive rate (R 0 ), intrinsic growth rate (r) and finite rate of increase (λ), and a shorter interval between generations (T) than on Pera, Natal and Hamlin oranges and Ponkan mandarin. On the other hand, mites reared on Hamlin orange had the lowest R 0 , r and λ and the highest T among the hosts. Based on the results obtained we recommend that for Valencia orange and Sicilian lemon, the mite monitoring programs should be more intense to detect the initial infestation of pest, avoiding the damage in plants and the increase in production costs.

  5. Volatile constituents of redblush grapefruit (Citrus paradisi) and pummelo (Citrus grandis) peel essential oils from Kenya.

    Science.gov (United States)

    Njoroge, Simon Muhoho; Koaze, Hiroshi; Karanja, Paul Nyota; Sawamura, Masayoshi

    2005-12-14

    The volatile constituents of cold-pressed peel essential oils of redblush grapefruit (Citrus paradisi Macfadyen forma Redblush) and pummelo (Citrus grandis Osbeck) from the same locality in Kenya were determined by GC and GC-MS. A total of 67 and 52 compounds, amounting to 97.9 and 98.8% of the two oils, respectively, were identified. Monoterpene hydrocarbons constituted 93.3 and 97.5% in the oils, respectively, with limonene (91.1 and 94.8%), alpha-terpinene (1.3 and 1.8%), and alpha-pinene (0.5%) as the main compounds. Sesquiterpene hydrocarbons constituted 0.4% in each oil. The notable compounds were beta-caryophyllene, alpha-cubebene, and (E,E)-alpha-farnesene. Oxygenated compounds constituted 4.2 and 2.0% of the redblush grapefruit and pummelo oils, respectively, out of which carbonyl compounds (2.0 and 1.3%), alcohols (1.4 and 0.3%), and esters (0.7 and 0.4%) were the major groups. Heptyl acetate, octanal, decanal, citronellal, and (Z)-carvone were the main constituents (0.1-0.5%). Perillene, (E)-carveol, and perillyl acetate occurred in the redblush grapefruit but were absent from the pummelo oil. Nootkatone, alpha- and beta-sinensal, methyl-N-methylanthranilate, and (Z,E)-farnesol were prominent in both oils. PMID:16332132

  6. 76 FR 23449 - Citrus Canker, Citrus Greening, and Asian Citrus Psyllid; Interstate Movement of Regulated...

    Science.gov (United States)

    2011-04-27

    ... to movement. Citrus canker is a plant disease that is caused by the bacterium Xanthomonas citri subsp... quarantined for citrus canker are found in Sec. 301.75-6. Citrus greening, also known as Huanglongbing disease of citrus, is considered to be one of the most serious citrus diseases in the world. Citrus...

  7. 78 FR 63369 - Citrus Canker, Citrus Greening, and Asian Citrus Psyllid; Interstate Movement of Regulated...

    Science.gov (United States)

    2013-10-24

    ... citrus canker are found in Sec. 301.75-6. Citrus greening, also known as Huanglongbing disease of citrus, is considered to be one of the most serious citrus diseases in the world. Citrus greening is a... areas of the world where the disease is endemic, citrus trees decline and die within a few years and...

  8. Alteração na atividade de peroxidase e concentração de fenóis em microtangerinas (Citrus spp. infectadas por Phytophthora parasitica

    Directory of Open Access Journals (Sweden)

    José Ribamar Gusmão Araújo

    2008-02-01

    Full Text Available The present research aimed to characterize and evaluate species and varieties of small-fruited mandarins of Tanaka’s group (Citrus spp. with potential use as rootstocks, in relation to infection to the Phytophthora parasitica, by means of foliar determination of peroxidase activity and total phenolics content. It was used the following species: C. reshni Hort. ex Tan., C. sunki Hort. ex Tan., C. pectinifera Tan., C. crenatifolia Lush., C. amblycarpa Ochese, C. aurantium L, C. reticulata Blanco and C. limonia Osb. The Center of Citrus Germoplasm of Botucatu and Cordeirópolis provide all plant material. Four resistant varieties to the stem rot and root rot infections: Pectinifera, Crenatifolia, Sun Chu Shu (clone Kat 1004 and Cleopatra (clone 1 were evaluated. Susceptible plants infected by P. parasitica presented higher activity of peroxidase, while phenolics contents were lower in susceptible group.

  9. Karyological studies in ten species of Citrus (Linnaeus, 1753 (Rutaceae of North-East India

    Directory of Open Access Journals (Sweden)

    Marlykynti Hynniewta

    2011-11-01

    Full Text Available Ten Citrus (Linnaeus, 1753 species of North-East India have been karyo-morphologically analysed. All studied species had 2n=18 chromosomes without any evidence of numerical variation. All the chromosomes were found to be of metacentric and sub-metacentric in all the species; the morphology of the chromosomes showing size difference only. Symmetrical karyotype which does not have much difference in the ratio of longest to shortest chromosome in all the species was observed. Three species, C. grandis (Osbeck, 1757, C. reticulata (Blanco, 1837 and C. medica (Linnaeus, 1753 are identified as true basic species from asymmetry studies of karyotypes as they reflect on the primitive nature of their genomes. C. indica (Tanaka, 1937 occupies a special taxonomic position within the genus Citrus as a progenitor for other cultivated species

  10. Isoenzymatic polymorphism in Citrus spp. and Poncirus trifoliata (L. Raf. (Rutaceae

    Directory of Open Access Journals (Sweden)

    Novelli Valdenice Moreira

    2000-01-01

    Full Text Available Isoenzymatic polymorphism analysis was used to determine genetic variability among species and hybrids of Citrus spp. and one accession of Poncirus trifoliata (L. Raf. Ten enzymatic systems aspartate aminotransferase (AAT, acid phosphatase (ACP, leucine aminopeptidase (LAP, 6-phosphogluconate dehydrogenase (6-PGD, isocitrate dehydrogenase (IDH, phosphoglucoisomerase (PGI, phosphoglucomutase (PGM, diaphorase (DIA, shikimate dehydrogenase (SKD and peroxidase (PRX were analyzed. Twenty loci and 48 alleles were identified. Sweet orange cultivars (C. sinensis (L. Osbeck showed the highest polymorphism with the largest number of heterozygous loci, although the alleles of those loci were the same in all cultivars, with the exception of Westin and Lima graúda. Mandarins (C. reticulata Blanco exhibited diverse patterns, whereas Poncirus trifoliata (L. Raf. showed high variability with all Citrus species and hybrids. Exclusive phenotypes were observed in some enzymatic systems, and similar patterns were found among interspecific hybrids and their putative parents.

  11. Targeted cybridization in citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness.

    Science.gov (United States)

    Guo, W W; Prasad, D; Cheng, Y J; Serrano, P; Deng, X X; Grosser, J W

    2004-05-01

    CMS (cytoplasmic male sterility) can be controlled by the mitochondrion genome in higher plants, including Satsuma mandarin. Somatic fusion experiments in citrus combining embryogenic callus protoplasts of one parent with leaf protoplasts of a second parent often produce cybrid plants of the leaf parent, a phenomenon occurring most often with interspecific fusion combinations. In an attempt to practically exploit this cybridization phenomenon, we conducted somatic fusion experiments combining embryogenic suspension-derived protoplasts of Satsuma mandarin, Citrus unshiu Marc. cv. Guoqing No. 1 (G1), a male-sterile cultivar, with leaf protoplasts of other seedy types--Hirado Buntan Pink pummelo (HBP) [Citrus grandis (L.) Osbeck], Sunburst mandarin (C. reticulata Blanco), Orie Lee hybrid (C. reticulata cv. Clementine x Murcott tangor), and Murcott tangor [C. reticulata x C. sinensis (L.) Osbeck], respectively--in an attempt to generate seedless cybrids by the targeted transfer of CMS. The genetic identities of regenerated plants from all four parental combinations were determined by flow cytometry, SSR, CAPS (or PCR-RFLP), RFLP, and chloroplast-SSR analyses. Regenerated plants from the first three parental combinations were diploids, and the cybrid nature of G1 + HBP with the mitochondrion genome from G1 and the chloroplast genome from HBP was confirmed, whereas the cybrid nature of the remaining two combinations was difficult to confirm because of the close phylogenetic relatedness of both fusion parents, as expected. Plants from G1 + Murcott were confirmed as tetraploid somatic hybrids. This is the first report of targeted citrus cybrid production by symmetric fusion with male-sterile Satsuma as the callus parent and other seedy cultivars as the leaf parents. PMID:14730385

  12. Diversidade de moscas-das-frutas em pomares de citros no município de Araruama, RJ Diversity of fruit flies in citrus groves in the municipality of Araruama, RJ

    OpenAIRE

    Jorge Ferreira de Souza; Silvana Aparecida da Silva Souza; Elen de Lima Aguiar-Menezes; Fernando Antônio Abrantes Ferrara; Stenilson Araújo Nascimento; William Costa Rodrigues; Paulo César Rodrigues Cassino

    2008-01-01

    O objetivo deste estudo foi determinar as espécies de Tephritidae e Lonchaeidae (Diptera: Tephritoidea) de ocorrência em pomares de laranja doce (Citrus sinensis Osbeck) e tangerina (Citrus reticulata Blanco), no município de Araruama, RJ, durante o período de dezembro de 2002 a novembro de 2003. Os espécimes foram coletados em armadilhas McPhail contendo solução aquosa de proteína hidrolisada a 5% e em amostras de frutos de seis variedades de citros. Nas armadilhas, o total de 2.543 adultos ...

  13. Crescimento vegetativo de plantas cítricas no norte e noroeste do Paraná Vegetative growth of citrus trees in north an northwest of Parana

    Directory of Open Access Journals (Sweden)

    Neusa Maria Colauto Stenzel

    2005-12-01

    Full Text Available Este trabalho avaliou, em Paranavaí e Londrina-PR, os fluxos de crescimento vegetativo dos ramos de laranjeira 'Folha Murcha' enxertada sobre limoeiro 'Cravo' (Citrus limonia Osb., limoeiro 'Volcameriano' (Citrus volkameriana Ten. e Pasq., tangerineira 'Sunki' (Citrus sunki Hort. ex Tan., tangerineira 'Cleópatra' (Citrus reshni Hort. ex Tan. e laranjeira 'Pêra' sobre o porta-enxerto limoeiro 'Cravo'. Ocorreram seis fluxos de crescimento em Paranavaí e sete em Londrina, no inverno, primavera, verão e outono, para todas as combinações de copa/porta-enxerto. O comprimento final dos ramos (121,2 mm a 151,6 mm não foi influenciado pelas combinações copa/porta-enxerto em Londrina, mas em Paranavaí a combinação 'Pêra'/limoeiro 'Cravo' produziu ramos mais longos que as demais. O período de crescimento dos ramos variou de 25,5 a 37,8 dias e foi menor em Paranavaí do que em Londrina, para todas as combinações de copa/porta-enxerto. Os resultados são discutidos em relação às características edafoclimáticas dos dois locais.This work evaluated in Paranavaí and Londrina, PR, Brazil, the vegetative growth fluxes of branches of 'Folha Murcha' orange on 'Rangpur' lime (Citrus limonia Osb., 'Volkamer' lemon (Citrus volkameriana Ten. & Pasq., 'Sunki' mandarin (Citrus sunki Hort. ex Tan., 'Cleopatra' mandarin (Citrus reshni Hort. ex Tan., and 'Pêra' orange on 'Rangpur' lime rootstock. Six growth flushes were observed in Paranavaí, and seven were observed in Londrina, in winter, spring, summer, and autumn, for all scion/rootstock combinations. The final length of the branches (121.2 mm to 151.6 mm was not influenced by the scion/rootstock combinations in Londrina; in Paranavaí, however, the 'Pêra'/'Rangpur' lime combination produced longer branches than the others. The growth period of branches ranged from 25.5 to 37.8 days and was shorter in Paranavaí than in Londrina, for all scion/rootstock combinations. The results are discussed

  14. 76 FR 8603 - Citrus Seed Imports; Citrus Greening and Citrus Variegated Chlorosis

    Science.gov (United States)

    2011-02-15

    ... disease of citrus and referred to below as HLB) is considered to be one of the most serious citrus... world where the disease is endemic, citrus trees decline and die within a few years and may never.... Manjunath. Asian Citrus Psyllids (Sternorrhyncha: Psyllidae) and Greening Disease of Citrus: A...

  15. Flutuação populacional de Phyllocnistis citrella Stainton em Citrus deliciosa e no híbrido Murcott Citrus sinensis x Citrus reticulata Phyllocnistis citrella Stainton population fluctuation in Citrus deliciosa and Murcott hybrid Citrus sinensis x Citrus reticulata

    Directory of Open Access Journals (Sweden)

    Cristiane Ramos de Jesus

    2008-06-01

    Full Text Available O objetivo desta pesquisa foi avaliar a dinâmica populacional de Phyllocnistis citrella Stainton, (Lepidoptera: Gracillariidae, o minador-dos-citros, em pomares de tangerineira Citrus deliciosa Tenore variedade Montenegrina e de tangoreiro híbrido "Murcott" Citrus sinensis L. Osbeck X Citrus reticulata Blanco, com manejo orgânico, em Montenegro (29° 68'S e 51° 46'O, Rio Grande do Sul. Foram realizadas amostragens quinzenais de julho de 2001 a junho de 2003. Os brotos coletados foram examinados em laboratório e submetidos à análise do número de folhas por broto, a da presença ou ausência de minas, do número de minas, dos ovos, das larvas e das pupas de P. citrella. Em ambos os pomares não houve registro de minas de P. citrella no primeiro fluxo de brotação, de agosto a outubro. No ano I, as maiores densidades de minas foram registradas em meados de novembro, início de janeiro e início de abril, em ambos os pomares. No ano II, constataram-se as maiores densidades de minas e larvas em janeiro e em abril, em C. deliciosa, e de dezembro a março em "Murcott". Embora o número médio de brotos registrado tenha sido sempre maior em C. deliciosa, a colonização e o estabelecimento do minador-dos-citros seguiram o mesmo padrão em ambos os pomares. A temperatura mínima e média e a umidade relativa do ar foram os fatores abióticos que apresentaram maior influência no número de minas e de larvas de P. citrella.To evaluate the population dynamics of Phyllocnistis citrella Stainton, (Lepidoptera: Gracillariidae, the citrus leafminer, in tangerine Citrus deliciosa Tenore var. Montenegrina and tangor 'Murcott' Citrus sinensis L Osbeck X Citrus reticulata Blanco in organically managed orchards, in Montenegro (29° 68'S e 51° 46'W, RS, fortnightly samples were carried out from July 2001 to June 2003. Sampled shoots were examined in the lab and the number of leaves, presence or absence of mines and the number of mines, eggs, larvae and

  16. PHARMACOLOGICAL IMPORTANCE OF CITRUS FRUITS

    OpenAIRE

    Amita Tomar *, Mridula Mall and Pragya Rai

    2013-01-01

    This paper reviews the pharmacological importance of citrus fruits. Citrus fruits are used for various pharmacological importance. According to literature the citrus fruit possess anti-cancer, antimicrobial, antioxidant, antiulcer, anti-inflammatory, and hypolipidemic and hepatoprotective properties.

  17. Citrus Waste Biomass Program

    Energy Technology Data Exchange (ETDEWEB)

    Karel Grohman; Scott Stevenson

    2007-01-30

    Renewable Spirits is developing an innovative pilot plant bio-refinery to establish the commercial viability of ehtanol production utilizing a processing waste from citrus juice production. A novel process based on enzymatic hydrolysis of citrus processing waste and fermentation of resulting sugars to ethanol by yeasts was successfully developed in collaboration with a CRADA partner, USDA/ARS Citrus and Subtropical Products Laboratory. The process was also successfully scaled up from laboratory scale to 10,000 gal fermentor level.

  18. CONSTITUENTS FROM LIMONIA CRENULATA

    Institute of Scientific and Technical Information of China (English)

    XUE-MEI NIU; SHENG-HONG LI; LI-YAN PENG; ZHONG-WEN LIN; GAO-XIONG RAO; HAN-DONG SUN

    2001-01-01

    A new indole alkaloid, crenulatine (1), along with twenty known compounds, was isolated from the stems of Lirnonia Crenulata. Their structures were identified by spectral means. Those compounds include four alkaloids, four coumarins, two flavanones, three tetranortriterpenoids,one triterpenoid, three steroids, two lignans and two aromatic compounds.

  19. Ulcer protective potential of standardized hesperidin, a citrus flavonoid isolated from Citrus sinensis

    Directory of Open Access Journals (Sweden)

    Papiya Bigoniya

    2014-06-01

    Full Text Available Free radicals play an important role in stomach ulcer formation. The present investigation validates the anti ulcer activity of hesperidin, isolated from Citrus sinensis (L. Osbeck, Rutaceae, through the assessment of its antioxidant potential over stomach mucosal tissue by histological examination. Hesperidin was isolated from the dried peel of C. sinensis, and authenticated by TLC, IR and HPLC. The anti-ulcerogenic potential of this fruit was assessed using indomethacin and hypothermic restrain stress-induced ulceration models on rats at 150, 300 and 450 mg/kg dose orally. The parameters measured were gastric pH, volume, free and total acidity, ulcer index, and mucin, glutathione, super oxide dismutase, catalase and protein content. Hesperidin at 300 and 450 mg/kg dose showed significant (p < 0.01-0.001 increase in pH, decrease in acidity and ulcer index against indomethacin and hypothermic restrain stress, along with histological evidence of cytoprotection. Glutathione, super oxide dismutase, catalase and mucin levels increased significantly at 450 mg/kg (p <0.05-0.001 after indomethacin ulceration, whereas hypothermic restrain stress only increased glutathione and mucin levels. Hesperidin prevents oxidative cell injury by significant rise of super oxide dismutase, glutathione and catalase levels in gastric mucosa. Hesperidin allowed the regeneration of ulcerated tissue, and prevented hemorrhagic injury of gastric mucosa. The potential anti-ulcer effect of hesperidin may be due to antioxidant, mucoprotective and cytoprotective activities.

  20. RAPD-based genetic analysis of offsprings from the sexual cross using allotetraploid citrus somatic hybrid as pollen parent

    Institute of Scientific and Technical Information of China (English)

    YI HuaLin; DENG XiuXin

    2007-01-01

    Huazhong Agricultural University, Wuhan 430070, ChinaThirty-one polymorphic decamer primers were selected to genotype 92 progenies from the cross between Yiben No,4, a monoembryonic diploid F1 hybrid of Citrus reticulata Blanco cv Huanongbendizao tangerine and C. ichangensis Swingle, and [Hamlin sweet orange + Rough lemon], an allotetraploid somatic hybrid of Citrus sinensis Osbeck cv. Hamlin and C. jambhiri Lush cv. Rough Lemon. x2(Chi-square) analysis of RAPD markers in the progenies indicated they were randomly transmitted from the four donor parents, without significant difference between the diploids and triploids. However,these progenies were clustered into three major groups using dendrogram constructed by UPGMA,skewed to three parents in certain degrees, 15 (13 triploids and 2 diploids) to Hamlin, 16 (9 and 7) to Yiben No. 4, and 61 (57 and 4) to [Hamlin sweet orange + Rough Lemon] from which genomic contribution was predominant in progenies, respectively.

  1. RAPD-based genetic analysis of offsprings from the sexual cross using allotetraploid citrus somatic hybrid as pollen parent

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Thirty-one polymorphic decamer primers were selected to genotype 92 progenies from the cross be- tween Yiben No.4, a monoembryonic diploid F1 hybrid of Citrus reticulata Blanco cv Huanongbendizao tangerine and C. ichangensis Swingle, and [Hamlin sweet orange + Rough lemon], an allotetraploid somatic hybrid of Citrus sinensis Osbeck cv. Hamlin and C. jambhiri Lush cv. Rough Lemon. χ2 (Chi-square) analysis of RAPD markers in the progenies indicated they were randomly transmitted from the four donor parents, without significant difference between the diploids and triploids. However, these progenies were clustered into three major groups using dendrogram constructed by UPGMA, skewed to three parents in certain degrees, 15 (13 triploids and 2 diploids) to Hamlin, 16 (9 and 7) to Yiben No. 4, and 61 (57 and 4) to [Hamlin sweet orange + Rough Lemon] from which genomic contribu- tion was predominant in progenies, respectively.

  2. Genetic similarity of citrus fresh fruit market cultivars Similaridade genética de cultivares de citros de mesa

    Directory of Open Access Journals (Sweden)

    Roberto Pedroso de Oliveira

    2005-08-01

    Full Text Available The objective of this work was to assess the genetic similarity of the following citrus fresh fruit market seedless cultivars: Lane Late, Navelate, Navelina and Salustiana sweet oranges (Citrus sinensis (L. Osbeck, Clemenules and Marisol mandarins (C. reticulata Blanco and Okitsu satsuma mandarin (C. unshiu Marcovitch, and the hybrids Nova [C. clementina x (C. paradisi x C. tangerina] and Ortanique (tangor probably derived from C. sinensis (L. Osbeck x C. reticulata Blanco, utilizing isoenzymatic markers. Electrophoresis analysis of proteins extracted from leaf tissues was utilized to detect polymorphisms at ten isoenzymatic systems. Out of 30 alleles, 16 were polymorphic. The Jaccard coefficient was utilized to estimate the genetic similarity between the cultivars and the unweigthed pair-group method using an arithmetic average (UPGMA was used to obtain the phenogram (NTSYS 1.7. The cultivars showed high genetic similarity (>72.5%, and were classified in five main groups: sweet oranges, 'Clemenules' and 'Marisol' mandarins, 'Nova', 'Ortanique', and 'Okitsu' satsuma mandarin.O objetivo deste trabalho foi estudar a similaridade genética das seguintes cultivares sem sementes de citros de mesa: laranjas Lane Late, Navelate, Navelina e Salustiana (Citrus sinensis (L. Osbeck, tangerinas Clemenules e Marisol (C. reticulata Blanco, satsuma Okitsu (C. unshiu Marcovitch e híbridos Nova [C. clementina x (C. paradisi x C. tangerina] e Ortanique (tangor provavelmente entre C. sinensis (L. Osbeck e C. reticulata Blanco, por intermédio de marcadores isoenzimáticos. O polimorfismo foi detectado por eletroforese de proteínas extraídas de tecido foliar em 10 sistemas isoenzimáticos. Foram obtidos 30 alelos, sendo 16 polimórficos. O coeficiente de Jaccard foi utilizado para estimar a similaridade genética entre as cultivares e o método UPGMA para gerar o fenograma por meio do NTSYS 1,7. As cultivares apresentaram elevada similaridade genética (>72,5% e

  3. Cercosporoid diseases of Citrus.

    Science.gov (United States)

    Huang, Feng; Groenewald, J Z; Zhu, Li; Crous, P W; Li, Hongye

    2015-01-01

    Citrus leaves and fruits exhibiting disease symptoms ranging from greasy spot, yellow spot, small or large brown spot, black dot, and brown dot were sampled from Fujian, Guangdong, Guizhou, Hunan, Jiangxi, Yunnan, Zhejiang provinces and the Guangxi Zhuang Autonomous Region in China. In total 82 isolates representing various cercosporoid genera were isolated from these disease symptoms, which were supplemented with eight Citrus cercosporoid isolates collected from other countries. Based on a morphological and phylogenetic study using sequence data from the nuclear ribosomal DNA's ITS1-5.8S-ITS2 regions (ITS), and partial actin (act), β-tubulin (tub2), 28S nuclear ribosomal RNA (28S rDNA) and translation elongation factor 1-α (tef1) genes, these strains were placed in the following genera: Cercospora, Pallidocercospora, Passalora, Pseudocercospora, Verrucisporota and Zasmidium. All isolates tended to be sterile, except the Zasmidium isolates associated with citrus greasy spot-like symptoms, which subsequently were compared with phylogenetically similar isolates occurring on Citrus and other hosts elsewhere. From these results four Zasmidium species were recognized on Citrus, namely Z. indonesianum on Citrus in Indonesia, Z. fructicola and Z. fructigenum on Citrus in China and Z. citri-griseum, which appears to have a wide host range including Acacia, Citrus, Eucalyptus and Musa, as well as a global distribution.

  4. In vitro organogenesis in some citrus species Organogênese in vitro em algumas espécies de cítrus

    Directory of Open Access Journals (Sweden)

    Evandro Henrique Schinor

    2011-06-01

    Full Text Available In vitro organogenesis of Citrus was studied for the genotypes Citrus sinensis cv. 'Natal', C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP - 0.0; 0.5; 1.0; 1.5 or 2.0 mg L-1. For the recalcitrant genotypes C. limonia and C. aurantium the in vitro organogenesis was also studied with internodal segments-derived explants, cultured in MT salts and vitamins medium supplemented with 0; 0.5; 1.0; 2.0, or 4.0 mg L-1 of BAP. The efficiency of culture medium supplementation with the combination of BAP (0.0; 1.0, or 2.0 mg L-1 and NAA (1-naphthaleneacetic acid - 0.0; 0.3, or 0.5 mg L-1 in the development of adventitious shoots was evaluated for C. aurantium. Culture medium supplementation with BAP is not essential for the adventitious shoots development in the four genotypes studied when epicotyl segments-derived explants are used. In general, culture media supplementation with BAP decreased the percentage of responsive explants excepted for C. sinensis cv. 'Natal' and C. limonia when the concentrations of 1.5 and 2.0 mg/L were used. The presence of cytokinin, in concentrations up to 2 mg/L, stimulated the in vitro organogenesis when internodal segments-derived explants were used for C. limonia and C. aurantium. For C. aurantium no adventitious shoots developed in explants (internodal segments cultured in basal culture medium, without BAP supplementation. Although no statistic differences could be detected, culture media supplementation with the combination of BAP and NAA favored the development of adventitious shoots in C. aurantium. The best concentration of NAA varied according to BAP concentration. The results presented herein, show that Citrus in vitro organogenesis depends on the interaction of culture medium composition, explant differentiation level, and genotype.A organogênese in vitro

  5. The role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation.

    Science.gov (United States)

    Peng, Gang; Wang, Chunyan; Song, Song; Fu, Xiumin; Azam, Muhammad; Grierson, Don; Xu, Changjie

    2013-10-01

    Three 1-deoxy-D-xylulose-5-phosphate synthases (DXS) and three phytoene synthases (PSY) were identified in citrus, from Affymetrix GeneChip Citrus Genome Array, GenBank and public orange genome databases. Tissue-specific expression analysis of these genes was carried out on fruit peel and flesh, flower and leaf of Satsuma mandarin (Citrus unshiu Marc.) in order to determine their roles in carotenoid accumulation in different tissues. Expression of CitDXS1 and CitPSY1 was highest in all test tissues, while that of CitDXS2 and CitPSY2 was lower, and that of CitDXS3 and CitPSY3 undetectable. The transcript profiles of CitDXS1 and CitPSY1 paralleled carotenoid accumulation in flesh of Satsuma mandarin and orange (Citrus sinensis Osbeck) during fruit development, and CitPSY1 expression was also associated with carotenoid accumulation in peel, while the CitDXS1 transcript level was only weakly correlated with carotenoid accumulation in peel. Similar results were obtained following correlation analysis between expression of CitDXS1 and CitPSY1 and carotenoid accumulation in peel and flesh of 16 citrus cultivars. These findings identify CitPSY1 and CitDXS1 as the main gene members controlling carotenoid biosynthesis in citrus fruit. Furthermore, chromoplasts were extracted from flesh tissue of these citrus, and chromoplasts of different shape (spindle or globular), different size, and color depth were observed in different cultivars, indicating chromoplast abundance, number per gram tissue, size and color depth were closely correlated with carotenoid content in most cultivars. The relationship between carotenoid biosynthesis and chromoplast development was discussed.

  6. Fuel from citrus waste

    Energy Technology Data Exchange (ETDEWEB)

    1979-08-01

    University of Florida researchers are looking for methods of using citrus peel to produce fuel for the citrus industry by using the methane gas given off in a special continuous digestion vessel. They are trying to discover ways to reduce the peel oil content of oranges as this oil inhibits the growth of organisms which produce the methane. The citrus industry currently utilizes orange peel and pulp to make cattle feed, but since the peel has to be dried this step consumes a lot of energy.

  7. Monitoring the viability of citrus rootstocks seeds stored under refrigeration

    Directory of Open Access Journals (Sweden)

    Sérgio Alves de Carvalho

    2013-03-01

    Full Text Available The citrus nursery tree is produced through the bud grafting process, in which rootstock is usually grown from seed germination. The objective of this research was to evaluate, in two dissimilar environmental conditions, the viability and polyembryony expression of five citrus rootstocks seeds stored in different periods under refrigeration. The rootstock varieties evaluated were: Rangpur lime (Citrus limonia Osb. cv. Limeira, Trifoliate orange (Poncirus trifoliata Raf. cv. Limeira, Citrumelo (P. trifoliata x C. paradisi Macf. cv. Swingle, Sunki mandarin (C. sunki Hort. ex Tanaka and Volkamer lemon (C. volkameriana Ten. & Pasq. cv. Catania 2. The experimental design was the randomized blocks in a 11 x 5 x 2 factorial scheme, evaluating from time zero to the tenth month of storage, the five varieties of rootstock in two environments: germination and growth B.O.D type chamber (Biological Oxygen Demand - Eletrolab Brand Model FC 122 at 25 °C; and greenhouse seedbed with partial temperature control (22 °C to 36 °C and humidity control (75-85%. The plot had 24 seeds in four replicates, using trays with substrate in greenhouse and Petri dishes with filter paper in B.O.D. chamber. The seed germination rate and polyembryony expression were evaluated monthly. It was concluded that Trifoliate and Citrumelo Swingle seeds can be stored for up to seven months, while Volkamer lemon, Rangpur lime and Sunki seeds can be stored for up to ten months. The polyembryony expression rate was slightly higher when measured in greenhouse than in B.O.D. chamber and remained stable in both environments until the seventh month, from which dropped sharply. Citrumelo Swingle seeds expressed the highest polyembryony rate (18.8%, followed by Rangpur lime and Volkamer lemon (average value of 13.7%, Sunki (9.4% and Trifoliate (3.2%. Despite some differences among varieties, the viability of rootstock stored seeds can be monitored either in the greenhouse or in B

  8. Effects of open-top chambers on Valencia' orange trees. [Citrus sinensis

    Energy Technology Data Exchange (ETDEWEB)

    Olszyk, D.M. (Environmental Protection Agency, Corvallis, OR (United States)); Takemoto, B.K. (California Air Resources Board, Sacramento (United States)); Kats, G.; Dawson, P.J.; Morrison, C.L.; Preston, J.W.; Thompson, C.R. (Univ. of California, Riverside (United States))

    Open-top field chambers are the most widely used technology for evaluating the impacts of air pollutants on vegetation. This study was conducted to evaluate the long-term effects of chambers on Valencia orange trees (Citrus sinensis (L.) Osbeck). The trees were exposed to ambient ozone (O{sub 3}) for 51 months in large (4.3-m diam. by 2.9-m high) nonfiltered open-top chambers (NF) and in ambient air without chambers (AA). Results suggest that the yield increases for NF compared to AA trees could, in part, be accounted for by decreased flux of O{sub 3} into leaves (based on decreased O{sub 3} exposure and leaf conductance). However, other factors, i.e., increased tree growth, altered leaf C allocation, and lack of wind stress occurring only in chambers, likely contributed to higher NF tree yields.

  9. Effects of pectinase clarification treatment on phenolic compounds of pummelo (Citrus grandis l. Osbeck) fruit juice.

    Science.gov (United States)

    Shah, Nor Nadiah Abdul Karim; Rahman, Russly Abdul; Shamsuddin, Rosnah; Adzahan, Noranizan Mohd

    2015-08-01

    The purpose of this study is to investigate the changes occured on phenolic compounds between two Malaysian varieties of pummelo fruit juice: Ledang (PO55) and Tambun (PO52) post-enzymatic clarification. The changes in polyphenols composition were monitored using High Performance Liquid Chromatography Diode Array Detection and Folin Ciocalteu's method. Clarification treatment of pummelo fruit juice with a commercial pectinase was optimized based on incubation temperature, time and enzyme concentration. Both varieties of pummelo fruit juice were treated with different optimized variables which produced the highest clarities with the least effect to the juice physical quality. Tambun variety was found to have significantly more total phenolic compounds (p authenticity and the health benefits from the juice. PMID:26243926

  10. The protective effects of pomelo extract (Citrus grandis L. Osbeck) against fructose-mediated protein oxidation and glycation

    OpenAIRE

    Caengprasath, Natarin; Ngamukote, Sathaporn; Mäkynen, Kittana; Adisakwattana, Sirichai

    2013-01-01

    Chronic hyperglycemia induces non-enzymatic protein glycation, which plays an important role in the development of diabetic complications. Immense efforts have been made to determine effective antiglycation compounds from natural products. Pomelo has shown beneficial effects for human health. The objective of this study was to determine the antiglycation effect of pomelo extract against fructose-mediated protein oxidation and glycation. Our results showed that the pomelo extract (0.25 - 2.00 ...

  11. In vitro bioaccessibility of carotenoids, flavonoids, and vitamin C from differently processed oranges and orange juices [Citrus sinensis (L.) Osbeck].

    Science.gov (United States)

    Aschoff, Julian K; Kaufmann, Sabrina; Kalkan, Onur; Neidhart, Sybille; Carle, Reinhold; Schweiggert, Ralf M

    2015-01-21

    Carotenoid, flavonoid, and vitamin C concentrations were determined in fresh orange segments and a puree-like homogenate derived thereof, as well as freshly squeezed, flash-pasteurized, and pasteurized juices. Lutein and β-cryptoxanthin were slightly degraded during dejuicing, whereas β-carotene levels were retained. Vitamin C levels remained unaffected, whereas flavonoid levels decreased 8-fold upon juice extraction, most likely due to the removal of flavonoid-rich albedo and juice vesicles. Likewise, the presence of such fibrous matrix compounds during in vitro digestion was assumed to significantly lower the total bioaccessibility (BA) of all carotenoids from fresh fruit segments (12%) as compared to juices (29-30%). Mechanical disruption of orange segments prior to digestion did not alter carotenoid BA, whereas pasteurization of the freshly squeezed juice slightly increased BA by 9-11%. In addition to carotenoid BA, the stabilities of hesperidin, narirutin, and vitamin C including dehydroascorbic acid during in vitro digestion were monitored, and applied analytical methods were briefly validated.

  12. Citrus diseases with global ramifications including citrus canker and huanglongbing

    Science.gov (United States)

    Although there are a number of diseases that plague citrus production worldwide, two bacterial diseases are particularly problematic. Both are of Asian origin and currently cause severe economic damage: Asiatic citrus canker (ACC) and citrus huanglongbing (HLB). Although ACC has been found in the ...

  13. Acción Bactericida del Jugo de Limón Sobre el Staphylococcus Aureus Rosenbach

    Directory of Open Access Journals (Sweden)

    Obregón Botero. Rafael

    1946-04-01

    Full Text Available El objeto de este experimento fue el determinar la influencia que pudiera tener el jugo de limón común (citrus limonia Osbeck sobre el Staphylococcus; aureus Rosenbach. Esta bacteria se encuentra con frecuencia en la piel y el pelo del hombre y de los animales, también en el pus y con frecuencia asociados a muchas otras causantes de abcesos, forúnculos, nacidos, etc. Ocasionalmente pueden causar osteomielitis y endocarditis. En individuos tuberculosos las infecciones causadas por esta bacteria pueden ocasionar la muerte.

  14. Acción bactericida del jugo de limón sobre el staphylococcus aureus rosenbach

    OpenAIRE

    Obregón Botero Rafael

    2012-01-01

    El objeto de este experimento fue el determinar la influencia que pudiera tener el jugo de limón común (citrus limonia Osbeck sobre el Staphylococcus; aureus Rosenbach. Esta bacteria se encuentra con frecuencia en la piel y el pelo del hombre y de los animales, también en el pus y con frecuencia asociados a muchas otras causantes de abcesos, forúnculos, nacidos, etc. Ocasionalmente pueden causar osteomielitis y endocarditis. En individuos tuberculosos las infecciones causadas por esta bacteri...

  15. Caracterização química e atividade antibacteriana de óleos essenciais de plantas condimentares e medicinais contra Staphylococcus aureus e Escherichia coli

    OpenAIRE

    Millezi, A. F.; N.N. Baptista; D.S. Caixeta; D.F. Rossoni; M.G Cardoso; R.H. Piccoli

    2014-01-01

    Os óleos essenciais são metabólitos secundários dos vegetais com propriedades biológicas diferenciadas, dentre elas, a atividade contra microrganismos, sendo de interesse da indústria de alimentos as concentrações mínimas inibitórias (CMI) desses óleos para diversas bactérias. As CMI variam em função dos compostos majoritários e da espécie de bactéria. Nesta pesquisa, os óleos essenciais das plantas Satureja montana L., Cymbopogon nardus L. e Citrus limonia Osbeck foram caracterizados quimica...

  16. PHARMACOLOGICAL IMPORTANCE OF CITRUS FRUITS

    Directory of Open Access Journals (Sweden)

    Amita Tomar *, Mridula Mall and Pragya Rai

    2013-01-01

    Full Text Available This paper reviews the pharmacological importance of citrus fruits. Citrus fruits are used for various pharmacological importance. According to literature the citrus fruit possess anti-cancer, antimicrobial, antioxidant, antiulcer, anti-inflammatory, and hypolipidemic and hepatoprotective properties.

  17. Skewed RAPD markers in linkage maps of Citrus

    Directory of Open Access Journals (Sweden)

    Roberto Pedroso de Oliveira

    2004-01-01

    Full Text Available The objective of this work was to analyze the effects of RAPD markers with skewed segregation on genetic linkage maps. Segregation data for 123 Citrus sinensis (L. Osbeck cv. Pêra markers and 53 C. reticulata Blanco cv. Cravo markers in F1 progeny composed of 94 hybrids were used. Genetic linkage maps of the two varieties were constructed with non-skewed markers (p < 0.05 and p < 0.01 using the program MAPMAKER 3.0 and a pseudo-testcross strategy. The maps were compared to those constructed with all markers. Alterations in the genetic distances were observed based on the location of the skewed markers within the linkage groups. Generally, the skewed markers were located at the end of the linkage groups, sometimes forming entire linkage groups, without causing significant distance modifications. However, skewed markers located between non-skewed markers caused significant distance modifications and, in some cases, altered the order of the markers. Most of the skewed markers can be included in linkage maps, but in each case the degree of distance modification caused by each marker needs to be assessed.

  18. Desarrollo vegetativo de patrones cítricos cultivados en condiciones de invernadero bajo dos sistemas de riego Vegetative development of citrus seedlings cultivated at greenhouse conditions and submitted to two irrigations systems

    Directory of Open Access Journals (Sweden)

    Gilmar Schäfer

    2006-08-01

    Full Text Available En el presente estudio se evaluó el desarrollo vegetativo de patrones cítricos cultivados en invernadero bajo dos sistemas de riego. El experimento se realizó en la Estação Experimental Agronômica de la Universidade Federal do Rio Grande do Sul, ubicada en Eldorado do Sul, Rio Grande do Sul, Brasil, entre los meses de septiembre de 2003 y abril de 2004, totalizando 225 días de experimentación. El diseño experimental fue de parcelas subdivididas, en factorial 2 x 3, con 4 repeticiones de 22 contenedores cada. En las parcelas principales se evaluaron los sistemas de riego (microaspersión y capilaridad y en las subparcelas los patrones cítricos Poncirus trifoliata (L. Raf., citrangero 'C37' [P. trifoliata x Citrus sinensis (L. Osb. cv. Pêra] y lima 'Rangpur' (C. limonia Osb.. En condiciones de invernadero los patrones cítricos presentan un desarrollo vegetativo más rápido bajo riego por capilaridad respecto a la microaspersión. Los patrones cítricos evaluados presentan desarrollos vegetativos distintos, donde el citrangero 'C37' supera a los demás.The aim of the present work was to evaluate the vegetative development of citrus rootstock seedlings cultivated under greenhouse conditions with two irrigation systems. The experiment was conducted at the Estação Experimental Agronômica , Universidade Federal do Rio Grande do Sul, located in Eldorado do Sul, Rio Grande do Sul, Brazil, from September 2003 to April 2004, totalizing 225 days of experimentation. The experimental design was a split-plot, in a 2x3 factorial, with 4 replications of 22 pots each. In the main plot the irrigation systems was evaluated (micro sprinkler and capillarity and in the split-plot the citrus rootstocks [Trifoliate orange - Poncirus trifoliata (L. Raf., 'C37' citrange - P. trifoliata x Citrus sinensis (L. Osb. cv. Pêra and 'Rangpur' lime - C. limonia Osb.] were evaluated. The main result showed in conditions of greenhouse citrus rootstock seedlings

  19. 77 FR 59709 - Citrus Greening and Asian Citrus Psyllid; Quarantine and Interstate Movement Regulations

    Science.gov (United States)

    2012-10-01

    ...) 851-2286. SUPPLEMENTARY INFORMATION: Background Citrus greening, also known as Huanglongbing disease of citrus, is considered to be one of the most serious citrus diseases in the world. Citrus greening... citrus greening disease. In areas of the world where the disease is endemic, citrus trees decline and...

  20. Antioxidant activity of Citrus fruits.

    Science.gov (United States)

    Zou, Zhuo; Xi, Wanpeng; Hu, Yan; Nie, Chao; Zhou, Zhiqin

    2016-04-01

    Citrus is well-known for its nutrition and health-promotion values. This reputation is derived from the studies on the biological functions of phytochemicals in Citrus fruits and their derived products in the past decades. In recent years, the antioxidant activity of Citrus fruits and their roles in the prevention and treatment of various human chronic and degenerative diseases have attracted more and more attention. Citrus fruits are suggested to be a good source of dietary antioxidants. To have a better understanding of the mechanism underlying the antioxidant activity of Citrus fruits, we reviewed a study on the antioxidant activity of the phytochemicals in Citrus fruits, introduced methods for antioxidant activity evaluation, discussed the factors which influence the antioxidant activity of Citrus fruits, and summarized the underlying mechanism of action. Some suggestions for future study were also presented. PMID:26593569

  1. Antioxidant activity of Citrus fruits.

    Science.gov (United States)

    Zou, Zhuo; Xi, Wanpeng; Hu, Yan; Nie, Chao; Zhou, Zhiqin

    2016-04-01

    Citrus is well-known for its nutrition and health-promotion values. This reputation is derived from the studies on the biological functions of phytochemicals in Citrus fruits and their derived products in the past decades. In recent years, the antioxidant activity of Citrus fruits and their roles in the prevention and treatment of various human chronic and degenerative diseases have attracted more and more attention. Citrus fruits are suggested to be a good source of dietary antioxidants. To have a better understanding of the mechanism underlying the antioxidant activity of Citrus fruits, we reviewed a study on the antioxidant activity of the phytochemicals in Citrus fruits, introduced methods for antioxidant activity evaluation, discussed the factors which influence the antioxidant activity of Citrus fruits, and summarized the underlying mechanism of action. Some suggestions for future study were also presented.

  2. 湖南柑橘大实蝇对不同柑橘品种的危害特点研究初报%The Damage Characteristics of Tetradacus citri Chen on Different Citrus Varieties in Hunan Province

    Institute of Scientific and Technical Information of China (English)

    林文力; 杨水芝; 潘美山; 陈海玲; 黄泽培; 龙建国; 肖伏莲

    2011-01-01

    采用定点观察和普查的方式对湖南省吉首市、麻阳县柑橘大实蝇的危害特点进行了研究.结果表明:柑橘大实蝇对不同柑橘品种的危害时间由早到晚依次为:脐橙>温洲蜜柑>冰糖橙≥大红甜橙=广柑>椪柑;其危害最严重的品种是脐橙,虫果率达63%,其次是早熟温洲蜜柑和冰糖橙,达22%,再次是椪柑,达6%;同时还发现,柑橘不同品种果皮表面存在产卵假痕迹现象.这些结果可为柑橘大实蝇的防治提供理论参考依据.%The damage characteristics of Tetradacus citri Chen in Mayang County, Jishou City, Hunan Province were investigated by fixed-point observation method and general survey method. The results indicated that the harm time of Tetradacus citri Chen on different varieties of citrus ordered as navel orange>satsuma mandarin>Citrus sinensis ^Citrus sineasis Osbeck cv. Dahong= Citrussinensis (L.) Osbeck >Citrus reticulata Blanco cv. Ponkan; the damage level of Tetradacus citri Chen on different varieties of citrus ordered as navel orange>early mature satsuma mandarin and Citrus sinensis >dtrus reticulata Blanco cv. Ponkan, and the insect infestation ratio of navel orange, early mature satsuma mandarin, Citrus sinensis and Citrus reticulata Blanco cv. Ponkan was 63%, 22%, 22% and 6%, respectively. Meanwhile, the research also showed that there are fake oviposition traces of Tetradacus citri Chen on the Peels of different varieties of citrus. These results above can provide the theory basis for the control of Tetradacus citri Chen.

  3. Citrus stubborn disease (CSD)

    Science.gov (United States)

    CSD is caused by Spiroplasma citri, a phloem-limited, cell-wall-less bacterium. S. citri is transmitted in a propagative, circulative manner by several leafhoppers including Circulifer tenellus and Scaphytopius nitridus in citrus-growing regions of California and Arizona and by C. haematoceps (syn....

  4. Cercosporoid diseases of Citrus

    NARCIS (Netherlands)

    Huang, Feng; Groenewald, J.Z.; Zhu, Li; Crous, P.W.; Li, Hongye

    2015-01-01

    Citrus leaves and fruits exhibiting disease symptoms ranging from greasy spot, yellow spot, small or large brown spot, black dot, and brown dot were sampled from Fujian, Guangdong, Guizhou, Hunan, Jiangxi, Yunnan, Zhejiang provinces and the Guangxi Zhuang Autonomous Region in China. In total 82 isol

  5. Potential Nutritional Benefits of Current Citrus Consumption

    OpenAIRE

    Tami Turner; Burri, Betty J.

    2013-01-01

    Citrus contains nutrients and phytochemicals that may be beneficial for health. We collected citrus production and consumption data and estimated the amount of these compounds that are consumed. We then compared the amounts of citrus and citrus-derived compounds used in studies that suggest a health benefit to the amounts typically found in citrus. Data is scarce, but suggests that citrus consumption might improve indices of antioxidant status, and possibly cardiovascular health and insulin s...

  6. Potential Nutritional Benefits of Current Citrus Consumption

    Directory of Open Access Journals (Sweden)

    Tami Turner

    2013-03-01

    Full Text Available Citrus contains nutrients and phytochemicals that may be beneficial for health. We collected citrus production and consumption data and estimated the amount of these compounds that are consumed. We then compared the amounts of citrus and citrus-derived compounds used in studies that suggest a health benefit to the amounts typically found in citrus. Data is scarce, but suggests that citrus consumption might improve indices of antioxidant status, and possibly cardiovascular health and insulin sensitivity.

  7. Physiological changes and expression characteristics of ZIP family genes under zinc deifciency in navel orange (Citrus sinensis)

    Institute of Scientific and Technical Information of China (English)

    XING Fei; FU Xing-zheng; WANG Nan-qi; XI Jian-long; HUANG Yi; ZHOU Wei; LING Li-li; PENG Liang-zhi

    2016-01-01

    Zinc (Zn) deifciency is widespread among citrus plants, but information about the mechanisms for Zn deifciency response in these plants is scarce. In the present study, different navel orange (Citrus sinensis (L.) Osbeck) leaves with various yelowing levels were sampled in our experimental orchard, and upon estimation of nutrient contents, Zn deifciencies were diagnosed as mild, moderate, and severe. Further analysis of chlorophyl content, photosynthetic characteristics, antioxidant enzyme activities, and expression levels ofZn/Iron-regulated transporter-like protein (ZIP) family genes were conducted in the sampled Zn-deifcient leaves. The results showed that chlorophyl contents and net photosynthetic rate (Pn) seemed to decrease with reduced Zn contents. In addition, comparison of severe Zn-deifcient and normal leaves revealed that activities of peroxidase (POD) and catalase (CAT) increased signiifcantly, whereas that of Zn-containing enzymes such as Cu/Zn superoxide dismutase (Cu/Zn-SOD) signiifcantly reduced with decreasing Zn contents. As expected, expression of the ZIP family genes,ZIP1,ZIP3, andZIP4, was induced by Zn deifciencies. These results deepen our understanding of Zn deifciency in citrus plants as wel as provide useful preliminary information for further research.

  8. Construction and characterization of two Citrus BAC libraries and identification of clones containing the phytoene synthase gene.

    Science.gov (United States)

    Baig, M N R; Yu, An; Guo, Wenwu; Deng, Xiuxin

    2009-05-01

    Two deep-coverage Bacterial Artificial Chromosome (BAC) libraries of Citrus sinensis (L.) Osbeck 'Cara Cara' navel orange and Citrus reticulata (L.) Blanco 'Egan No. 1' Ponkan mandarin, which belong to the two most important species of the Citrus genus, have been constructed and characterized to facilitate gene cloning and to analyze variety-specific genome composition. The C. sinensis BAC library consists of 36 000 clones with negligible false-positive clones and an estimated average insert size of 126 kb covering ~4.5 x 109 bp and thus providing an 11.8-fold coverage of haploid genome equivalents, whereas the C. reticulata library consists of 21 000 clones also with negligible false-positive clones and an estimated average of 120 kb covering ~2.5 x 109 bp representing a 6.6-fold coverage of haploid genome equivalents. Both libraries were evaluated for contamination with high-copy vector, empty pIndigoBAC536 vector, and organellar DNA sequences. Screening has been performed by Southern hybridization of BAC filters, which results in genomics research in the two important species C. sinensis and C. reticulata. Resources, high-density filters, individual clones, and whole libraries are available for public distribution and are accessible at the National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University.

  9. Somatic embryogenesis in Citrus sinensis, C. reticulata AND C. nobilis x C. deliciosa

    Directory of Open Access Journals (Sweden)

    Ricci Adriana Patrícia

    2002-01-01

    Full Text Available Most of the plant regeneration processes in citrus, through tissue culture, involve indirect somatic embryogenesis. The optimization of these processes is important for the development of in vitro plant improvement and micropropagation studies. Studies to evaluate the effect of different carbohydrates in somatic embryogenesis were conducted using calli from 'Ponkan' mandarin (Citrus reticulata, Blanco, 'Cravo' mandarin (C. reticulata, 'Itaboraí' sweet orange (C. sinensis L. Osbeck., 'Valencia' sweet orange (C. sinensis and 'Kinnow' mandarin (C. nobilis Loureiro x C. deliciosa Tenore. The culture medium used was MT supplemented with sucrose, galactose, glucose, maltose or lactose with the following concentrations of 18, 37, 75, 110, and 150 mM. The culture medium used for the maturation of somatic embryos had 0, 15, 29, 44, 58 and 73 mM of sucrose, in presence or absence of 0.5 g L-1 of activated charcoal. Seventy-three mM of sucrose with 0.1 mg L-1 of GA3 in the presence or absence 0.5 g L-1 of activated charcoal was also tested. Overall, the carbohydrates galactose or lactose induced a higher number of somatic embryos. Sucrose concentrations of 58 and 73 mM generated a higher number of plantlets from mature embryos of 'Ponkan' mandarin and 'Valencia' sweet orange.

  10. Índice de qualidade em um latossolo amarelo coeso cultivado com citros Quality index in a cohesive yellow latosol cultivated with citrus

    Directory of Open Access Journals (Sweden)

    José Fernandes de Melo Filho

    2009-12-01

    Full Text Available O Brasil é o maior produtor mundial de citros e exportador de suco concentrado de laranja. Na região Nordeste, a citricultura tem grande importância social, onde é cultivada predominantemente por produtores com áreas menores do que 10 hectares, em solos como caráter coeso. Na Bahia, a citricultura é explorada sobre diversas condições tecnológicas, e especialmente nos Tabuleiros Costeiros os pomares têm baixa longevidade e produtividade em função das limitações dos solos coesos à produção agrícola. Este trabalho tem como objetivo determinar a qualidade física e química de um Latossolo Amarelo Coeso cultivado com citros. O estudo foi realizado na região do Recôncavo Baiano, em um pomar comercial de laranja "Pera" (Citrus sinensis L. Osb. sob porta-enxerto de limão-cravo (Citrus limonia L. Osb.. Para a determinação do índice de qualidade do solo (IQS, utilizou-se o método proposto por Karlen & Stott (1994, e amostras foram coletadas em duas camadas: 0,00 - 0,20 m e 0,20 - 0,40 m. Os resultados mostraram que o Latossolo Amarelo Coeso sob manejo tradicional para a cultura de citros apresentou índice de qualidade regular, com limitações determinadas pela elevada resistência do solo à penetração, baixa permeabilidade à água e baixo teor de matéria orgânica, o que resulta em limitações para permitir o crescimento e o aprofundamento do sistema radicular e prover o fornecimento e a disponibilidade de água para as plantas cítricas.Brazil is the greatest citrus producer and exporter of concentrated orange juice in the world. In the Northeast, the citriculture has a large social importance for farming family, which is predominantly cultivated for small farmers. In Bahia state the citriculture has been exploited under several technology conditions and especially in the Costal table lands soil the orchards have low longevity and productivity based on the cohesive soil limitations for rural production. The purpose of

  11. Phytochemical analysis and radical scavenging profile of juices of Citrus sinensis, Citrus anrantifolia, and Citrus limonum

    OpenAIRE

    Rauf, Abdur; Uddin, Ghias; Ali, Jawad

    2014-01-01

    Background The aim of the current investigation was to identify bioactive secondary metabolites including phenols, tannins, flavonoids, terpinedes, and steroids and compare the phytochemical analysis and antioxidant profile of the juice extracted from the fruits of Citrus sinensis, Citrus anrantifolia, and Citrus limonum. Results Phytochemical screening is important for the isolation of new, novel, and rare secondary metabolites before bulk extraction. Phytochemical analysis of the desired pl...

  12. Valuing Catastrophic Citrus Losses

    OpenAIRE

    Adams, Damian C.; Kilmer, Richard L.; Moss, Charles B.; Schmitz, Andrew

    2004-01-01

    Courts are often required to estimate changes in welfare to agricultural operations from catastrophic events. For example, courts must assign damages in lawsuits, such as with pesticide drift cases, or determine "just compensation" when the government takes private land for public use, as with the removal of dairy farms from environmentally sensitive land or destruction of canker-contaminated citrus trees. In economics, the traditional method of quantifying producer losses is estimating chang...

  13. 'Valencia' sweet orange tree flowering evaluation under field conditions Avaliação do florescimento de laranjeiras valência em condição de campo

    Directory of Open Access Journals (Sweden)

    Rafael Vasconcelos Ribeiro

    2008-01-01

    Full Text Available Since citrus flowering is a key process in citriculture and its evaluation is often difficult due to the canopy structure and field sampling, the aim of this research was to give some directions regarding the evaluation of flowering in field-grown sweet orange plants. This study was conducted in a citrus orchard of sweet orange plants cv. 'Valencia' [Citrus sinensis (L. Osbeck] grafted on 'Cleopatra' mandarin (Citrus reshni hort. ex Tanaka or 'Rangpur' lime (Citrus limonia Osbeck rootstocks, with North-South orientation. Generative structures [buds, flowers and fruitlets (diameter O florescimento dos citros é um processo chave na citricultura e sua avaliação é dificultada devido à estrutura da copa e amostragem em campo. O objetivo desse artigo foi fornecer algumas indicações de como avaliar o florescimento de laranjeiras em condição de campo. Esse estudo foi conduzido em um pomar de laranjeiras 'Valência' [Citrus sinensis (L. Osbeck] enxertadas em tangerineira 'Cleópatra' (Citrus reshni hort. ex Tanaka ou limoeiro 'Cravo' (Citrus limonia Osbeck, com orientação Norte-Sul. As estruturas reprodutivas [botões florais, flores e frutos (diâmetro < 3 cm] foram quantificadas semanalmente entre agosto e novembro de 2005, utilizando guias de 1 m² posicionadas no terço médio da copa das plantas, amostrando aproximadamente um volume de 1 m³. As guias foram divididas em duas partes para que duas pessoas pudessem realizar as avaliações, e posicionadas nas orientações sudeste, sudoeste, nordeste e noroeste, em sete plantas. Alguns aspectos do florescimento dos citros foram avaliados: (i quantas plantas são necessárias para uma amostragem representativa do florescimento; (ii em qual orientação deve ser feita a medida e (iii qual volume da copa das plantas que deve ser amostrado. Ao se considerar os aspectos práticos da produção dos citros, um método rápido, simples e representativo é necessário para avaliar o florescimento

  14. Multimodal cues drive host-plant assessment in Asian citrus psyllid (Diaphorina citri).

    Science.gov (United States)

    Patt, Joseph M; Meikle, William G; Mafra-Neto, Agenor; Sétamou, Mamoudou; Mangan, Robert; Yang, Chenghai; Malik, Nasir; Adamczyk, John J

    2011-12-01

    Asian citrus psyllid (Diaphorina citri) transmits the causal agent of Huanglongbing, a devastating disease of citrus trees. In this study we measured behavioral responses of D. citri to combinations of visual, olfactory, and gustatory stimuli in test arenas. Stimuli were presented to the psyllids in droplets or lines of an emulsified wax formulation in two different arena types in no-choice tests. First, when placed on a colored ring situated halfway between the center and perimeter of a petri dish, D. citri spent more time on yellow versus gray rings; however, this response disappeared when either gray or yellow wax droplets were applied. When the psyllids were presented with droplets scented with terpenes, the response to both scent and color was increased. The addition of a dilute (≍0.1 M) sucrose solution to the wax droplets increased the magnitude of D. citri responses. Next, groups of D. citri were placed on plastic laboratory film covering a sucrose solution, to mimic a leaf surface. Test stimuli were presented via two 'midribs' made from lines of emulsified wax formulation. Probing levels were measured as a function of color saturation and scent composition, and concentration. The test scents were based on qualitatively major volatiles emitted by Murraya paniculata (L.) Jack, Citrus aurantifolia (Christm.) Swingle, and C. sinensis (L.) Osbeck. The highest probing response was observed on the middle concentration (20-μl scent/10 ml wax formulation) of the C. aurantifolia-scented wax lines. Results indicate that there are interactive effects between the different sensory modalities in directing host-plant assessment behavior.

  15. Dispersion patterns and sampling plans for Diaphorina citri (Hemiptera: Psyllidae) in citrus.

    Science.gov (United States)

    Sétamou, Mamoudou; Flores, Daniel; French, J Victor; Hall, David G

    2008-08-01

    The abundance and spatial dispersion of Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were studied in 34 grapefruit (Citrus paradisi Macfad.) and six sweet orange [Citrus sinensis (L.) Osbeck] orchards from March to August 2006 when the pest is more abundant in southern Texas. Although flush shoot infestation levels did not vary with host plant species, densities of D. citri eggs, nymphs, and adults were significantly higher on sweet orange than on grapefruit. D. citri immatures also were found in significantly higher numbers in the southeastern quadrant of trees than other parts of the canopy. The spatial distribution of D. citri nymphs and adults was analyzed using Iowa's patchiness regression and Taylor's power law. Taylor's power law fitted the data better than Iowa's model. Based on both regression models, the field dispersion patterns of D. citri nymphs and adults were aggregated among flush shoots in individual trees as indicated by the regression slopes that were significantly >1. For the average density of each life stage obtained during our surveys, the minimum number of flush shoots per tree needed to estimate D. citri densities varied from eight for eggs to four flush shoots for adults. Projections indicated that a sampling plan consisting of 10 trees and eight flush shoots per tree would provide density estimates of the three developmental stages of D. citri acceptable enough for population studies and management decisions. A presence-absence sampling plan with a fixed precision level was developed and can be used to provide a quick estimation of D. citri populations in citrus orchards.

  16. 中药材威灵仙的研究进展%Research progress of Clematis chinensis Osbeck

    Institute of Scientific and Technical Information of China (English)

    典灵辉

    2004-01-01

    威灵仙,又称粉灵仙、灵仙、黑须公、铁灵仙等,为毛茛科铁线莲属威灵仙(Clematis chinensis Osbeck)的干燥根茎,属多年生草本植物。据《中国药典》记载威灵仙(Clematis chinensis Osbeck)、棉团铁线莲(Clematis hexapetala Pall.)或东北铁线莲(Clematis manshurica Rupr.)为正品。威灵仙味辛成,性温,有微毒,具有祛风除湿,通络止痛之功效。临床上主要用于风湿痹痛,肢体麻木,筋脉挛急,屈伸不利等症状。

  17. Basal respiration and stratification ratio in soil cultivated with citrus and treated with organic residues in the state of Sergipe

    Directory of Open Access Journals (Sweden)

    Juliana Augusta Moura

    2015-04-01

    Full Text Available The use of organic residues in the soil to improve its quality, provide nutrients to plant growth and increase carbon storage is an ancient practice, but little is known about the application of laminar composting in citrus orchards. The objective of this study was to evaluate the microbial activity through microbial respiration, and the different rates stratification of some variables related to this attribute of an Ultisol cultivated with citrus and treated with organic waste in the state of Sergipe. Soil samples were collected in the layers 0-5 cm, 5-10 cm, and 10-15 cm in a citrus orchard (Citrus sinensis, L. Osbeck under different treatments 1. Layering organic residue (crop wastes placed in layers under the plant canopy 2. Layering organic residue + NPK (the same as described in iten 1 + NPK; 3. Humus (Humified organic compost applied under the plant canopy; 4. Humus + NPK (the same as described in iten 3 + NPK; 5. Control + NPK (plant canopy was kept free of residues, + NPK application; 6. Control – NPK (plant canopy was kept free of residues, without application of NPK, with three replications. It has been found that the use of the organic residue in the plant canopy increased microbial respiration rate and total organic matter content, as well as an increase in some chemical attributes as pH, P, Ca and Mg in all treatments compared the control. The ratio stratification was effective to evaluate the influence of different management practices between depths demonstrating an improvement in microbial activity by more practical conservation treatments and indicating that the use of Humus, humus + NPK and alternate layering residues + NPK were the most effective in increasing the organic matter content in the soil

  18. Citrus peel waste treatment

    Energy Technology Data Exchange (ETDEWEB)

    Yamanouchi, I.; Kuno, H.

    1977-07-30

    Citrus peel waste from canneries are treated with an acid and an additional base then fermented by anaerobic bacteria to produce CH/sub 4/. Then the solution is aerobically treated and discharged. The excess activated sludge is recycled for fermentation. Thus, a mixture of mandarin-orange cannery waste (80% water) and 0.5% HCl was heated at 120/sup 0/ to hydrolyze the organic matter to obtain a solution containing glucose and suspended solids. The solution is then enriched with N and P for fermentation to produce 50 L of CH/sub 4/ in 5 days vs 25 L without HCl.

  19. Antihyperlipidemic effects of Citrus sinensis, Citrus paradisi, and their combinations

    OpenAIRE

    Neelam Mallick; Rafeeq Alam Khan

    2016-01-01

    Objective: Hyperlipidemia, extensively contributes in the progression of coronary heart diseases and atherosclerosis, but may be managed through alterations in the nutritional pattern. Several studies show that diet rich in polyphenols and antioxidants have antiatherogenic effects. Citrus sinensis and Citrus paradisi are widely known for health benefits and have found to produce antioxidant, anti-inflammatory, and hypolipidemic effects, hence current research was planned to determine the hypo...

  20. Relationship Between Leaf Vein Splitting and Mineral Nutrition of Citrus%柑橘叶脉开裂症与矿质营养的关系

    Institute of Scientific and Technical Information of China (English)

    李健; 谢钟琛; 谢文龙; 吴兴明; 施清

    2011-01-01

    柑橘叶脉开裂症属于多病因的病症,缺Mg或缺B均可导致发病,对'纽荷尔'脐橙[Citrus sinensis(L.)Osbeck'Newhall']的缺Mg叶脉开裂症病株,在生长季4月采用1.0%硝酸镁叶面喷施矫治2~3次,可有效降低病叶发生.通过福建柑橘产区普查,由缺Mg引起叶脉开裂症占86.2%,其次为B与Mg共同缺乏,缺B仅占2.3%.不同品种缺Mg叶脉开裂的感病顺序为:纽荷尔脐橙>琯溪蜜柚,其它发病品种还有金柑、早熟温州蜜柑、瓯柑.采用易感品种纽荷尔脐橙与抗性品种椪柑互为中间砧高接比对发现,纽荷尔脐橙对Mg和B的吸收能力低于椪柑,对K的吸收高于椪柑,这可能是纽荷尔脐橙易患叶脉开裂症的原因.缺Mg和缺B病症的最显著的区别为,缺Mg叶脉开裂多位于叶片项部"∧"形黄化部位;缺B叶脉开裂症病叶呈绿色不黄化;Mg、B缺乏症的病叶主脉和侧脉明显开裂与全叶黄化,或叶脉开裂达基部"∧"形绿色区域.%This study confirmed that leaf vein splitting of citrus was multi-pathogeny caused by both Mg-deficiency and B-deficiency. Spraying 1.0% Mg(NO3)2· 6H2O had significant effect on remedying leaf vein splitting of‘ Newhall’ [Citrus sinensis (L.) Osbeck] caused by Mg-deficiency in April. According to the investigation in Fujian citrus orchards, 86.2% of leaf vein splitting was suffered from Mg-deficiency, 11.5% from Mg-deficiency and B-deficiency, only 2.3% from B-deficiency. Infection rates of leaf vein splitting caused by Mg-deficiency ranked in descending order was as follow: Newhall navel orange, ‘Guanxi-miyou' [C. grandis (L.) Osbeck]. Other infected varieties included ‘Kumquat’(Fortunella crassifolia Swingle), Satsuma mandarin (C. unshiu Marc. ‘Early variety’ ) and ‘Ougan’(C. suavissima Hort. ex Tanaka). Leaf vein splitting susceptible variety Newhall [C. sinensis(L. )Osbeck]and resistant varieties Ponkan ( C. reticulate Blanco) were used as intermediate stocks in graft

  1. Diversidade e dinâmica populacional de ácaros em pomar cítrico Diversity and population dynamics of mites in citrus orchard

    OpenAIRE

    Marcos Zatti da Silva; Mário Eidi Sato; Carlos Amadeu Leite de Oliveira

    2012-01-01

    As plantas de citros hospedam diversas espécies de ácaros que causam sérios prejuízos à produção e qualidade de frutos no Brasil. Esta pesquisa teve por objetivo estudar a diversidade de ácaros e a dinâmica populacional das principais espécies de ácaros em pomar de citros, Citrus sinensis (L.) Osbeck, localizado no município de Descalvado, Estado de São Paulo. Foram realizadas 14 avaliações em um período de 12 meses, de fevereiro de 2007 a fevereiro de 2008. Em cada avaliação, foram coletadas...

  2. EFEITO DO ARMAZENAMENTO DE HASTES DE LARANJA CACAU (Citrus sinensis Osb NO "PEGAMENTO" DE BORBULHAS EFFECT OF THE STORAGE PERIOD OF “CACAU” ORANGE (Citrus sinensis OSB BUDWOODS ON THE QUALITY OF BUDS FOR GRAFTING

    Directory of Open Access Journals (Sweden)

    Maria Terezinha Teixeira Lopes

    2007-09-01

    Full Text Available

    O presente trabalho foi realizado na Escola de Agronomia e Veterinária da UFGO, com o objetivo de estudar o efeito da conservação de borbulhas de laranja “Cacau” (Citrus sinensis Osb embaladas em sacos plásticos, no pegamento de enxertos. Os tratamentos utilizados foram enxertia com borbulhas de hastes com 0, 5, 10, 15 e 20 dias de conservação. A percentagem de enxertos brotados decresceu linearmente em função do período de conservação. À medida que aumentava a intensidade de descoloração das hastes, diminuía a percentagem de "pegamento" de enxertos. Para nossas condições as borbulhas de laranja "cacau" (Citrus sinensis Osb não devem ser conservadas em sacos plásticos, à temperatura ambiente, por mais de 5 dias, pois a percentagem de enxertos brotados decresceu linearmente com o período de conservação das hastes.

    This work was carried out at the Escola de Agronomia e Veterinária da UFGO. This study was made to test the effect of the storage period of the “cacau” orange budwood on the quality of buds for grafting. Budwoods of the “Cacau” orange (Citrus sinensis  Osb were harvested, packed in polyethylene bags and maintained at environmental temperature. The buds were grafted on rootstocks of Rangpur lime (Citrus limonia, Osb at intervals of 6 days (0, 5, 10, 15 and 20 days. It was noted that the loss of bud viability increased simultaneously with progressive change in budwood color from the natural green to an intense brown. The percentages of buds alive after 60 days of grafting were: 100% on the day budwoods were harvested; 70% after 5 days of storage; 36% after 10 days of storage and 00% after 15 days.

  3. 75 FR 34322 - Citrus Greening and Asian Citrus Psyllid; Quarantine and Interstate Movement Regulations

    Science.gov (United States)

    2010-06-17

    ... known as Huanglongbing disease of citrus, is considered to be one of the most serious citrus diseases in the world. Citrus greening is a bacterial disease, caused by strains of the bacterial pathogen... a tree with citrus greening disease. In areas of the world where the disease is endemic,...

  4. 78 FR 8435 - Importation of Fresh Citrus Fruit From Uruguay, Including Citrus Hybrids and Fortunella

    Science.gov (United States)

    2013-02-06

    ... From Uruguay, Including Citrus Hybrids and Fortunella spp., Into the Continental United States AGENCY... citrus fruit, as well as Citrus hybrids and the Citrus-related genus Fortunella, from Uruguay into the... certificate issued by the national plant protection organization of Uruguay with an additional...

  5. 柑橘木虱对柑橘不同品种的趋性%Diaphorina citri's Gost Preference on Thirteen Citrus Varieties

    Institute of Scientific and Technical Information of China (English)

    陈建利; 阮传清; 刘波; 范国成; 段永平; D.HALL

    2011-01-01

    用2种方法测定了柑橘木虱Diaphorina citri Kuw.成虫对13个柑橘品种离体嫩梢的选择性.结果表明,对不同品种进行两两组合的选择性试验,柑橘木虱停靠在处理组合早金和四季斑叶橘的虫量占总虫量的23%和1%,停靠在处理组合台湾玫瑰橙和纽荷尔脐橙上的虫量分别占总虫量的33%和20%,停靠在处理组合日辉和佩奇甜橙上虫量分别占总虫量的38%和15%,两两之间差异显著.将13个品种组合在一起进行选择性试验,结果表明柑橘木虱停靠在纽荷尔、福橘和佩奇甜橙上的成虫数量为每梢3.2~6.6只·d-1,显著高于木虱在其余10个品种枝梢上的停靠数量.福橘是柑橘黄龙病高发品种的原因是其对柑橘木虱的吸引力较强.%Under laboratory conditions, preference of adult Asian Citrus Psyllid (ACP), Diaphorina citri Kuw, for infestation among 13 citrus varieties was determined with two experimentations. Experiment A applied every 2 detached shoots of different citrus varieties and put together for choice. Experiment B used the detached shoots of all tested citrus varieties and put together for choice. The results of Experiment A revealed that the percentage of ACP adults on each shoot of Citrus sinensis (L.) Osbeck cv. Earlygold and C. medurensis (C. mitis) cv. Varigated Calamondin were 23 % and 1 %, respectively, which was significantly different from each other. The percentage on C. sinensis Osbeck cv. Taiwanmeiguicheng (33%) and cv. Newhall Navel Orange (20%) were also significantly different from each other. Significant different percentages of ACP adults were also observed between C. reticulata C. paradisi cv. Rihui (38%) and C. reticulata C. grandis cv. Page (15%). In Experiment B, the number of ACP on the shoots of C. sinensis Osbeck cv. Newhall Navel Orange, C. tangerina Tanaka cv. Fuju and C. reticulata Citrusgrandis cv. Page was 3.2-6.6 adults · shoot-day-1 , which were significantly higher than that

  6. Control of virus diseases of citrus.

    Science.gov (United States)

    Lee, Richard F

    2015-01-01

    Citrus is thought to have originated in Southeast Asia and horticulturally desirable clonal selections have been clonally cultivated for hundreds of years. While some citrus species have nucellar embryony, most cultivation of citrus has been by clonal propagation to ensure that propagated plants have the same traits as the parent selection. Clonal propagation also avoids juvenility, and the propagated plants produce fruit sooner. Because of the clonal propagation of citrus, citrus has accumulated a large number of viruses; many of these viruses are asymptomatic until a susceptible rootstock and/or scion is encountered. The viruses reported to occur in citrus will be summarized in this review. Methods of therapy to clean selected clones from viruses will be reviewed; the use of quarantine, clean stock, and certification programs for control of citrus viruses and other strategies to control insect spread citrus viruses, such as mild strain cross-protection and the use of pest management areas will be discussed.

  7. Levantamento de moscas-das-frutas e seus parasitoides em citros, no município de Viçosa, Minas Gerais Survey of fruit-flies and their parasitoids in citrus in Viçosa, Minas Gerais

    Directory of Open Access Journals (Sweden)

    Daniel Lucas Magalhães Machado

    2012-12-01

    Full Text Available As moscas-das-frutas são responsáveis por grandes perdas em fruteiras comerciais no Brasil, por isso é fundamental conhecer as espécies predominantes na região. Objetivou-se com este trabalho estudar a ocorrência de moscas-das-frutas (Diptera: Tephritidae e seus parasitoides em laranjas doces (Citrus sinensis L. Osbeck, tangerina Poncã (Citrus reticulata Blanco e mexerica Rio (Citrus deliciosa Ten, no município de Viçosa, Minas Gerais. Os frutos foram coletados em abril de 2008. No laboratório eles foram acondicionados em caixas plásticas contendo areia umedecida e em ambiente controlado para obtenção dos pupários, que foram contados, acondicionados em frascos de vidro com areia fina e mantidos em estufa até a emergência dos adultos. Somente uma espécie de mosca-das-frutas (Anastrepha fraterculus (Wiedemann, 1830 e uma de parasitoide (Doryctobracon brasiliensis Szépligeti foram identificadas. Dentre as variedades, a laranja doce Baianinha apresentou o maior índice de infestação, e os menores foram atribuídos à mexerica Rio e à tangerina Poncã.Fruit flies are responsible for large losses in commercial orchards in Brazil, thus, it is important to know the predominant species in the region. The objective of this study was to study the occurrence of fruit flies (Diptera: Tephritoidae and of their parasitoids in sweet orange (Citrus sinensis L. Osbeck, 'Poncã' mandarin (Citrus reticulate Blanco and 'Rio' tangerine (Citrus deliciosa Ten, in Viçosa, Minas Gerais. The fruits were collected in April 2008. In the laboratory, the fruits were stored in plastic boxes containing moist sand in a controlled environment, to obtain pupae. Then, the pupae were counted, placed in glass bottles with fine sand and kept in an oven until adult emergence. Only one species of fruit fly (Anastrepha fraterculus (Wiedemann, 1830 and one species of parasitoid (Doryctobracon brasiliensis Szépligeti were identified. Among the varieties, the sweet

  8. Diversidade de moscas-das-frutas em pomares de citros no município de Araruama, RJ Diversity of fruit flies in citrus groves in the municipality of Araruama, RJ

    Directory of Open Access Journals (Sweden)

    Jorge Ferreira de Souza

    2008-04-01

    Full Text Available O objetivo deste estudo foi determinar as espécies de Tephritidae e Lonchaeidae (Diptera: Tephritoidea de ocorrência em pomares de laranja doce (Citrus sinensis Osbeck e tangerina (Citrus reticulata Blanco, no município de Araruama, RJ, durante o período de dezembro de 2002 a novembro de 2003. Os espécimes foram coletados em armadilhas McPhail contendo solução aquosa de proteína hidrolisada a 5% e em amostras de frutos de seis variedades de citros. Nas armadilhas, o total de 2.543 adultos de Tephritoidea (1.430 fêmeas e 1.023 machos foi capturado, sendo dez espécies de Tephritidae, quatro espécies e dois morfotipos de Lonchaeidae. Dos Tephritidae e capturados nas McPhail, quatro espécies (Anastrepha fraterculus, A. obliqua, A. sororcula e Ceratitis capitata infestaram frutos cítricos, enquanto que, dos Lonchaeidae, somente os morfotipos não infestaram as amostras de citros. Os resultados demonstram que a densidade populacional das moscas-das-frutas pode ser superestimada, quando baseada no número de moscas por armadilha, devido à captura de espécies que não infestam os frutos de interesse comercial.This study was aimed at determining the species of Tephritidae and Lonchaeidae (Diptera: Tephritoidea that occur on groves of sweet orange (Citrus sinensis Osbeck and tangerine (Citrus reticulata Blanco in the municipality of Araruama, state of Rio de Janeiro, Brazil, from December, 2002 to November, 2003. The specimens were collected by McPhail traps with aqueous solution of hydrolyzed protein to 5% and in fruits samples of six varieties of citrus. In the traps the total of 2,543 adults of Tephritoidea (1,430 females and 1,023 males was captured, being ten species of Tephritidae, four species and two morphotypes of Lonchaeidae. From the Tephritidae caught by the McPhail, four species (Anastrepha fraterculus, A. obliqua, A. sororcula and Ceratitis capitata infested citric fruits while the Lonchaeidae, the morphotypes did not infest

  9. CIBMAN: Database exploring Citrus biodiversity of Manipur

    OpenAIRE

    Sanabam, Rakesh; Somkuwar, Bharat Gopalrao; Thingnam, Gourshyam; Moirangthem, Sila; Handique, Pratap Jyoti; Huidrom, Sunitibala

    2012-01-01

    The rich wealth of Citrus genetic resources makes India to enjoy a remarkable position in the “Citrus belt of the world”. We have developed CIBMAN, a unique database on Citrus biodiversity of Manipur which comprises 33 accessions collected through extensive survey for more than three years. CIBMAN provides integrated access to Citrus species through sophisticated web interface which has following capabilities a) morphological details, b) socio-economic details, c) taxonomic details and d) geo...

  10. Citrus tristeza virus-host interactions

    OpenAIRE

    Dawson, William O.; Garnsey, Stephen M.; Satyanarayana eTatineni; Folimonova, Svetlana Y.; Scott J Harper; S eGowda

    2013-01-01

    Citrus tristeza virus (CTV) is a phloem-limited virus whose natural host range is restricted to citrus and related species. Although the virus has killed millions of trees, almost destroying whole industries, and continually limits production in many citrus growing areas, most isolates are mild or symptomless in most of their host range. There is little understanding of how the virus causes severe disease in some citrus and none in others. Movement and distribution of CTV differs considerably...

  11. Citrus breeding, genetics and genomics in Japan

    OpenAIRE

    Omura, Mitsuo; Shimada, Takehiko

    2016-01-01

    Citrus is one of the most cultivated fruits in the world, and satsuma mandarin (Citrus unshiu Marc.) is a major cultivated citrus in Japan. Many excellent cultivars derived from satsuma mandarin have been released through the improvement of mandarins using a conventional breeding method. The citrus breeding program is a lengthy process owing to the long juvenility, and it is predicted that marker-assisted selection (MAS) will overcome the obstacle and improve the efficiency of conventional br...

  12. Soil Micronutrients and Citrus Growth

    Institute of Scientific and Technical Information of China (English)

    OUYANGTAO

    1993-01-01

    By using nutritional diagnosis of citrus leaves and determining soil micronutrients,the relationship between soil micronutrients and citrus growth in southern China has been studied.Studies showed that there was a significant positive correlation between available micronutrients (such as Zn,Mo,Cu)in the soil and the corresponding nutrients in citrus leaves.Thus,one can roughly learn of the sufficiency or deficiency of certain nutrients in soils by analyzing citrus leaves.Rational spray of Zn B or Mo fertilizer not only improved citrus yields but also increased the total sugar of Satsuma mandarin and of Xinhui orange by 2.9 and 17.2% respectively compared with the control.Spraying Mo fertilizer increased the vitamin C content of Satsuma mandarin juice by 4.7%-8.4%,maturated fruits 7-10 days earlier and gave the peel a brighter color.The ultramicroscopic characteristics of Zn-deficient citrus leaves were investigated under an electron microscope.Results showed that the Zn-deficient leaf cell was characterized mainly by poor cytoplasm,endoplasmic reticula and ribosomes and by big starch grains in the chloroplast.As a result of spraying Zn fertilizer the structure of the cell returned to normal,the cytoplasm became rich and the amount of chloroplast increased.There also appeared a great deal of multiform endoplasmic reticula,thus promoting the photosynthesis of Zn-deficient plants.This provides a cytologico-theoretical basis for fertilization of high-yielding citrus trees.

  13. Citrus Limonoids: Analysis, Bioactivity, and Biomedical Prospects

    Science.gov (United States)

    This publication is a review of the chemistry, biochemistry and bioactivity of limonoids occurring in citrus. The review chronologically relates the evolution of research in citrus limonoids beginning with their association with bitterness development in citrus juices. The chemical and biochemical...

  14. Chemical Constituents of Clematis chinensis Osbeck%威灵仙的化学成分研究

    Institute of Scientific and Technical Information of China (English)

    赵阳; 梁丽珍; 李厚金; 文诗雅; 蓝文健

    2012-01-01

    Clematis chinensis Osbeck is a popular medicinal plant. Seven known compounds, pinoresinol (1), epipinoresinol (2), matairesinol (3), salicifoliol (4), 3, 4, 5-trihydroxybenzoic acid (5), 4-hydroxy-3, 5-dimethoxybenzoic acid (6) , and 3-hydroxy- 4- methoxycinnamic acid (7) were isolated from the ethyl acetate extract of Clematis chinensis Osbeck by using the flash silica gel column chromatog-raphy and preparative high performance liquid chromatography. Their structures were elucidated mainly on the basis of NMR and MS data. These compounds could be fast and accurately detected from the extract by using HPLC and GCMS analytical methods. Lignans 1, 2, 3, 4 have various bioactivities, and the lignans from Clematis chinensis Osbeck should have the potential application in drug development.%威灵仙是一种重要的中药材.运用快速硅胶柱层析和制备型HPLC技术对威灵仙的乙酸乙酯提取物进行分离,纯化得到7个化合物,它们是松脂素( Pinoresinol,1),Epipinoresinol (2),罗汉松脂素(Matairesinol,3),Salicifoliol (4),3,4,5-三羟基苯甲酸(5),4-羟基-3,5-二甲氧基-苯甲酸(6),异阿魏酸(7),化合物的结构通过NMR和MS的分析得到确定.运用分析型HPLC和GC-MS色谱分析方法,可以在威灵仙药材提取物中快速、准确地鉴定该类化合物.化合物1,2,3,4是木脂素类化合物,具有多种重要的生理活性,潜在药用明显.

  15. Comparative morpho-anatomical studies of the lesions caused by citrus leprosis virus on sweet orange

    Directory of Open Access Journals (Sweden)

    João P.R. Marques

    2010-06-01

    Full Text Available The leprosis disease shows a viral etiology and the citrus leprosis virus is considered its etiologic agent. The disease may show two types of cytopatologic symptom caused by two virus: nuclear (CiLV-N and cytoplasmic (CiLV-C types. The aim of this study was to compare the morpho-anatomical differences in the lesions caused by leprosis virus-cytoplasmic and nuclear types in Citrus sinensis (L. Osbeck 'Pêra'. Leaf and fruit lesions were collected in Piracicaba/São Paulo (cytoplasmic type and Monte Alegre do Sul/São Paulo and Amparo/São Paulo (nuclear type. The lesions were photographed and then fixed in Karnovsky solution, dehydrated in a graded ethylic series, embedded in hydroxy-ethyl methacrylate resin (Leica Historesin, sectioned (5 μm thick, stained and mounted in synthetic resin. The digital images were acquired in a microscope with digital video camera. Leaf and fruit lesions caused by the two viruses were morphologically distinct. Only the lesion caused by CiLV-N virus presented three well-defined regions. In both lesions there was the accumulation of lipidic substances in necrotic areas that were surrounded by cells with amorphous or droplets protein. Only leaf and fruit lesions caused by CiLV-N virus exhibited traumatic gum ducts in the vascular bundles.A doença leprose dos citros tem etiologia viral sendo o citrus leprosis virus seu agente etiológico. Demonstrou-se que há dois vírus distintos que causam sintomas de leprose em ci-tros: citoplasmático (CiLV-C e o nuclear (CiLV-N. O objetivo desse estudo foi comparar as diferenças morfo-anatômicas nas lesões causadas por CiLV-C e por CiLV-N em laranjeira doce (Citrus sinensis (L. Osbeck 'Pêra'. As lesões foliares e dos frutos foram coletadas em Piracicaba/SP (tipo citoplas-mático e em Monte Alegre do Sul/SP e Amparo/SP (tipo nuclear. As lesões foram fotografadas e em seguida fixadas em solução Karnovsky, desidratadas em série etílica, incluídas em historesina e

  16. Citrus asymmetric somatic hybrids produced via fusion of gamma-irradiated and iodoacetamide-treated protoplasts

    Energy Technology Data Exchange (ETDEWEB)

    Bona, Claudine Maria de [Instituto Agronomico do Parana (IAPAR), Curitiba, PR (Brazil)], e-mail: debona@iapar.br; Gould, Jean Howe [Texas A and M University, College Station, TX (United States). Dept. of Ecosystem Science and Management], e-mail: gould@tamu.edu; Miller Junior, J. Creighton [Texas A and M University, College Station, TX (United States). Dept. of Horticultural Sciences], e-mail: jcmillerjr@tamu.edu; Stelly, David [Texas A and M University, College Station, TX (United States). Dept. of Soil and Crop Sciences], e-mail: stelly@tamu.edu; Louzada, Eliezer Silva [Texas A and M University, Kingsville, TX (United States). Citrus Center], e-mail: e-louzada@tamu.edu

    2009-05-15

    The objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma.irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad.) cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck) 'Itaborai', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow.) and 'Changsha' mandarin (C. reticulata Blanco) and 'Murcott' tangor (C. reticulata x C. sinensis). Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L{sup -1} iodoacetamide (IOA), and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto sour orange, rough lemon or 'Swingle' (C. paradisi x Poncirus trifoliata) x 'Sunki' mandarin rootstock seedlings, or rooted after dipping their bases in indol.butyric acid (IBA) solution. The products were later acclimatized to greenhouse conditions. Ploidy was analyzed by flow cytometry, and hybridity was confirmed by amplified fragment length polymorphism (AFLP) analysis of plantlet DNA samples. The best treatment was the donor-recipient fusion combination of 80 Gy.irradiated 'Ruby Red' protoplasts with 20 min IOA.treated 'Succari' protoplasts. Tetraploid and aneuploid plants were produced. Rooting recalcitrance was solved by dipping shoots' stems in 3,000 mg L{sup -1} IBA solution for 10 min. (author)

  17. Variety and Harvesting Season Effects on Antioxidant Activity and Vitamins Content of Citrus sinensis Macfad.

    Directory of Open Access Journals (Sweden)

    Vanessa Cardeñosa

    2015-05-01

    Full Text Available Five sweet orange (Citrus sinensis Osbeck varieties cultivated in Huelva (Spain and picked at two seasons during two consecutive years, were characterized for their antioxidant activity (free radicals scavenging activity, reducing power and lipid peroxidation inhibition and vitamin content (vitamin E and vitamin C. The effects induced by sweet orange variety and stage of maturity were comprehensively compared by applying 2-way ANOVA and linear discriminant analysis. The results indicated higher differences in antioxidant activity and vitamin contents in response to the effect of the harvesting season, when compared to the effect of sweet orange variety. Nevertheless, the results observed in 2012 showed less marked differences among the assayed sweet orange varieties. Either way, it might be concluded that oranges sampled in January show the highest antioxidant activity and vitamin contents. Furthermore, concerning the properties evaluated in this work, all sweet orange varieties represent good alternatives, except for Rhode Summer, which would not be the preferable choice as a target to enhance sweet orange overall characteristics.

  18. Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion.

    Science.gov (United States)

    Grosser, J W; Gmitter, F G; Tusa, N; Recupero, G R; Cucinotta, P

    1996-05-01

    Somatic hybridization experiments in Citrus that involve the fusion of protoplasts of one parent isolated from either nucellus-derived embryogenic callus or suspension cultures with leaf-derived protoplasts of a second parent, often result in the regeneration of diploid plants that phenotypically resemble the leaf parent. In this study, plants of this type regenerated following somatic fusions of the following three parental combinations were analyzed to determine their genetic origin (nuclear and organelle): (embryogenic parent listed first, leaf parent second) (1) calamondin (C. microcarpa Bunge) + 'Keen' sour orange (C. aurantium L.), (2) Cleopatra mandarin (C. reticulata Blanco) + sour orange, and (3) 'Valencia' sweet orange (C. sinensis (L.) Osbeck) + 'Femminello' lemon (C. limon (L.) Burm. f.). Isozyme analyses of PGI, PGM, GOT, and IDH zymograms of putative cybrid plants, along with RFLP analyses using a nuclear genome-specific probe showed that these plants contained the nucleus of the leaf parent. RFLP analyses using mtDNA-specific probes showed that these plants contained the mitochondrial genome of the embryogenic callus donor, thereby confirming cybridization. RFLP analyses using cpDNA-specific probes revealed that the cybrid plants contained the chloroplast genome of either one or the other parent. These results support previous reports indicating that acquisition of the mitochondria of embryogenic protoplasts by leaf protoplasts is a prerequisite for recovering plants with the leaf parent phenotype via somatic embryogenesis following somatic fusion. PMID:24178608

  19. Evaluation of central nervous system effects of Citrus limon essential oil in mice

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    Lidianne Mayra Lopes Campêlo

    2011-08-01

    Full Text Available The central nervous system (CNS depressant and anticonvulsant activities of Citrus limon (L. Osbeck, Rutaceae, essential oil (EO were investigated in animal models. The EO (50, 100 and 150 mg/kg injected by oral route (p.o. in mice caused a significant decrease in the motor activity of animals when compared with the control group, up to thirty days after the administration and the dose of 150 mg/kg significantly reduced the remaining time of the animals on the Rota-rod apparatus. Additionally, C. limon essential oil was also capable to promote an increase of latency for development of convulsions induced by pentylenetetrazole (PTZ. The administration of FLU (10 mg/kg, i.p., GABA A-benzodiazepine (GABA-BZD receptor antagonist, antagonized the effect of C. limon essential oil at higher dose. This C. limon essential oil was also capable to promote an increase of latency for development of convulsions induced by picrotoxin (PIC at higher dose. In the same way, the anticonvulsant effect of the EO was affected by pretreatment with flumazenil, a selective antagonist of benzodiazepine site of GABA A receptor. These results suggest a possible CNS depressant and anticonvulsant activities in mice that needs further investigation.

  20. Caracterização e atividade antibacteriana de óleos essenciais de plantas condimentares e medicinais contra Staphylococcus aureus e Escherichia coli

    Directory of Open Access Journals (Sweden)

    A.F. Millezi

    2013-01-01

    Full Text Available Os óleos essenciais são metabólitos secundários vegetais com propriedades biológicas diferenciadas, dentre elas a atividade contra microrganismos, sendo de importante interesse para a indústria de alimentos. As concentrações mínimas inibitórias (CMI desses óleos para diversas bactérias devem ser determinadas. As CMI variam de acordo com o óleo utilizado, dos compostos majoritários e do tipo de bactéria. Nesta pesquisa, os óleos essenciais das plantas Satureja montana L., Cymbopogon nardus L. e Citrus limonia Osbeck foram caracterizados quimicamente e determinada a CMI sobre as bactérias Staphylococcus aureus ATCC 2592 e Escherichia coli ATCC 25922. A CMI para todos os óleos contra E. coli foi 1,5%, já S. aureus foi sensível a partir da concentração de 5,0% do óleo essencial de S. montana e 1,5% foi a CMI obtida quando utilizados os óleos essenciais das outras espécies estudadas. Na constituição química os componentes majoritários para os óleos de S. montana, C. narduse C. limonia Osbeck foram respectivamente o timol, citronelal e limoneno.

  1. Caracterização química e atividade antibacteriana de óleos essenciais de plantas condimentares e medicinais contra Staphylococcus aureus e Escherichia coli

    Directory of Open Access Journals (Sweden)

    A.F. Millezi

    2014-03-01

    Full Text Available Os óleos essenciais são metabólitos secundários dos vegetais com propriedades biológicas diferenciadas, dentre elas, a atividade contra microrganismos, sendo de interesse da indústria de alimentos as concentrações mínimas inibitórias (CMI desses óleos para diversas bactérias. As CMI variam em função dos compostos majoritários e da espécie de bactéria. Nesta pesquisa, os óleos essenciais das plantas Satureja montana L., Cymbopogon nardus L. e Citrus limonia Osbeck foram caracterizados quimicamente e se determinou a CMI sobre as bactérias Staphylococcus aureus ATCC 2592 e Escherichia coli ATCC 25922. A CMI dos óleos testados contra E. coli e S. aureus foi de 1,5%, exceto para o óleo essencial de S. montana sobre S. aureus, a qual foi sensível a este óleo a partir da concentração de 5,0%. Sobre a constituição química, os componentes majoritários dos óleos de S. montana, C. narduse C. limonia Osbeck foram respectivamente o timol, citronelal e limoneno.

  2. Asymmetric somatic hybridization between UV-irradiated Citrus unshiu and C. sinensis: regeneration and characterization of hybrid shoots.

    Science.gov (United States)

    Xu, Xiao-Yong; Hu, Zhi-Yong; Li, Jun-Feng; Liu, Ji-Hong; Deng, Xiu-Xin

    2007-08-01

    In the present paper, attempts were made to explore the possibility of employing ultraviolet (UV) irradiation in citrus asymmetric fusion for transfer of limited amount of favorable traits from a desirable cultivar to a target one. Exposure of Satsuma mandarin (Citrus unshiu Marc.) embryogenic protoplasts to UV at an intensity of 300 muW cm(-2) led to reduced viability, especially under long irradiation duration. The protoplasts could not grow during culture when they were irradiated for over 30 s. Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assay revealed extensive DNA fragmentation in the UV-irradiated protoplasts compared with those without UV treatment. Electrofusion between UV-irradiated protoplasts of Satsuma mandarin (donor) with those of Jincheng (C. sinensis Osbeck, recipient), a local cultivar of superior quality, gave rise to regeneration of several lines of shoots, which failed to root despite enormous endeavors. Ploidy analysis via flow cytometry and chromosome counting showed that four selected shoots were either diploid, triploid or tetraploid. Random amplified polymorphism DNA (RAPD) and amplified fragment length polymorphism (AFLP) confirmed the shoots, irrespective of their ploidy level, as putative somatic hybrids. Cleaved amplified polymorphism sequences (CAPS) demonstrated that the shoots predominantly got their cytoplasmic components, in terms of chloroplast (cp) and mitochondrion DNA, from Jincheng, along with possible recombination of cpDNA in some shoot lines. The current data indicated that UV-based asymmetric fusion could also be employed in citrus somatic hybridization with the intention of creating novel germplasms, which may provide an alternative approach for cultivar improvement. PMID:17443330

  3. 柑桔果实糖酸比及线粒体乌头酸酶活性的变化%CHANGES IN SUGAR/ACID RATIO AND ACTIVITY OF MITOCHONDRIAL ACONIDASE IN DEVELOPING CITRUS FRUIT

    Institute of Scientific and Technical Information of China (English)

    罗安才; 李道高; 李纯凡

    2004-01-01

    测定了尤力克柠檬(高酸Citrus limon Burm.F)、冰糖橙(低酸C. sinensis Osbeck)、锦橙(中酸C. sinensis Osbeck)、奉节脐橙(C.sinensis Osbeck)72-1及其低酸和晚熟芽变在果实发育过程中还原糖含量、糖酸比以及线粒体乌头酸酶(Aconitase,EC 4.2.1.3)和苹果酸脱氢酶(Malate dehydrogenase,MDH,EC 1.1.1.37)活性变化.结果表明:各类型柑橘果实发育过程中糖的含量,随着果实的生长而增加,奉节脐橙的低酸芽变还原糖含量和糖酸比的增长速度最快,与其说它是低酸还不如说是早熟更为合理.各类型柑橘果实线粒体乌头酸酶活性变化一致随果实发育降低,表明果实发育后期有机酸降解与线粒体乌头酸酶活性的降低无关,而较低的细胞质乌头酸酶活性有利于酸的积累;柠檬MDH活性一直较低,但其他柑桔MDH活性变化与果实柠檬酸含量差异没有发现有明显的关系.

  4. Effectivity of Kaffir lime (Citrus hystrix, Nasnaran Mandarin (Citrus amblycarpa, and Pomelo (Citrus maxima Leaf Extract Against Aedes aegypti Larvae

    Directory of Open Access Journals (Sweden)

    Hebert Adrianto

    2014-03-01

    Full Text Available The used of chemicals as larvicidal may causing resistance, health problem, and environment problem. Leaf extracts of Citrus as biolarvicidal against Aedes aegypti larvae can be used as an alternative larvicidal. The aims of this study is to find out the species of Citrus in the study sample, which is the most effective as biolarvicidal. This study was conducted using nested experiment design, with six treatments and five replicates. Larvae mortality was observed after 24 and 48 hours. Then, data were analyzed by probit. The results of this study show that (1 the LC95 value after 24 hours of exposure of leaf extracts of Citrus hystrix, Citrus amblycarpa, and Citrus maxima, were each 3,176 ppm; 4,174 ppm; and 6,369 ppm. (2 the LC95 value after 48 hours of exposure of leaf extracts of Citrus hystrix, Citrus amblycarpa, and Citrus maxima, were each 2,499 ppm; 3,256 ppm; and 4,886 ppm. (3 leaf extract of Citrus hystrix is the most effective among others as biolarvicidal against Aedes aegypti larvae. Leaf extract of Citrus hystrix can be used as alternative biolarvicidal.

  5. Citrus Functional Genomics and Molecular Modeling in Relation to Citrus sinensis (Sweet Orange) Infection with Xylella fastidiosa (Citrus Variegated Chlorosis).

    Science.gov (United States)

    Dwivedi, Upendra N; Tiwari, Sameeksha; Prasanna, Pragya; Awasthi, Manika; Singh, Swati; Pandey, Veda P

    2016-08-01

    Citrus are among the economically most important fruit tree crops in the world. Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa infection, is a serious disease limiting citrus production at a global scale. With availability of citrus genomic resources, it is now possible to compare citrus expressed sequence tag (EST) data sets and identify single-nucleotide polymorphisms (SNPs) within and among different citrus cultivars that can be exploited for citrus resistance to infections, citrus breeding, among others. We report here, for the first time, SNPs in the EST data sets of X. fastidiosa-infected Citrus sinensis (sweet orange) and their functional annotation that revealed the involvement of eight C. sinensis candidate genes in CVC pathogenesis. Among these genes were xyloglucan endotransglycosylase, myo-inositol-1-phosphate synthase, and peroxidase were found to be involved in plant cell wall metabolism. These have been further investigated by molecular modeling for their role in CVC infection and defense. Molecular docking analyses of the wild and the mutant (SNP containing) types of the selected three enzymes with their respective substrates revealed a significant decrease in the binding affinity of substrates for the mutant enzymes, thus suggesting a decrease in the catalytic efficiency of these enzymes during infection, thereby facilitating a favorable condition for infection by the pathogen. These findings offer novel agrigenomics insights in developing future molecular targets and strategies for citrus fruit cultivation in ways that are resistant to X. fastidiosa infection, and by extension, with greater harvesting efficiency and economic value. PMID:27447755

  6. Incidência de Dilobopterus costalimai Young e Acrogonia citrina Marucci & Cavichioli, em pomares cítricos no noroeste paranaense = Incidence of Dilobopterus costalimai Young and Acrogonia citrina Marucci & Cavichioli, in citrus orchards in Northwestern Paraná

    Directory of Open Access Journals (Sweden)

    Aline Maria Orbolato Gonçalves

    2008-07-01

    Full Text Available As cigarrinhas das famílias Cicadellidae e Cercopidae são vetores da bactéria Xylella fastidiosa Wells, causadora da Clorose variegada dos citros. Esta doença traz sérios prejuízos à lavoura de citros no Noroeste Paranaense, e é estudada no Brasil desde 1987, quando foi descoberta pela primeira vez na região de Colina, São Paulo. Os vetores transmitem a X. fastidiosa quando se alimentam sugando a seiva do xilema, local onde a bactéria se instala, obstruindo a passagem da seiva. Este trabalho teve o objetivo de avaliar aocorrência das cigarrinhas vetoras Dilobopterus costalimai e Acrogonia citrina em pomar comercial de citros. O experimento foi realizado no sítio “Laranjeiras I”, município de Nova Esperança, região noroeste do Paraná, no período de julho de 2004 a agosto de 2006. Foram feitas amostragens mensais, por meio de armadilhas adesivas amarelas (Biocontrole®, nas variedades de laranjeiras [Citrus sinensis (L. Osbeck] Folha Murcha, Valência, Pêra e Natal.Nas variedades Pêra e Valência, a cigarrinha mais capturada foi Dilobopterus costalimai. Os meses de maior ocorrência das espécies foram entre dezembro e maio de cada ano.The sharpshooters of the families Cicadellidae and Cercopidae are vectors of Xylella fastidiosa (Wells, which causes citrus variegated chlorosis. This disease causes severe damage to the Paraná citrus industry, and it has been studied in Brazil since 1987, when it was discovered for the first time in the area of Colina, SP. The vectors transmit X. fastidiosa when they feed on the xylem sap, where the bacteria settles, obstructing the passage of the sap. This workhad the objective of evaluating the occurrence of the vector sharpshooter Dilobopterus costalimai and Acrogonia citrina in a commercial citrus orchard during the period between July 2004 and August 2006. The experiment was accomplished in the Laranjeiras I farm, in the city of Nova Esperança, northwestern Paraná. The collections

  7. Avaliação do potencial hídrico foliar, umidade do solo e temperatura do ar no período pré- florescimento dos citros Evaluation of the leaf water potencial, soil humidity and air temperature on the pre-bloom of citrus

    Directory of Open Access Journals (Sweden)

    Maria do Céu Monteiro da Cruz

    2007-10-01

    Full Text Available O trabalho foi conduzido em um pomar de citros localizado no Setor de Fruticultura do Departamento de Fitotecnia da Universidade Federal de Viçosa-UFV/Viçosa-MG, no período de março a setembro de 2004. O objetivo foi avaliar a influência do potencial hídrico das folhas e do solo sobre o florescimento da tangerineira 'Poncã', laranjeira 'Serra d'Água'e limeira ácida 'Tahiti' nas condições climáticas de Viçosa-MG. A temperatura do ar (ºC e a precipitação pluviométrica (mm foram avaliadas diariamente durante o período experimental. O potencial hídrico no solo e nas folhas foi avaliado em dois horários (7:00 às 8:00 h manhã e 13:00 às 14:00 h tarde. As cultivares utilizadas foram laranjeira 'Serra d'Água'(Citrus sinensis (L. Osb., tangerina 'Poncã' (Citrus reticulata Blanco e limeira ácida 'Tahiti' (Citrus latifolia Tanaka., enxertadas sobre limoeiro 'Cravo' (Citrus limonia Osb.. Observou-se que o potencial hídrico foliar dos citros diminuiu sob condições de altas temperaturas e déficit hídrico no solo, entretanto, varia em função dos cultivares, observando-se os maiores valores para a limeira 'Tahiti'. O florescimento ocorreu após um período de baixas temperaturas seguido por uma redução do potencial hídrico do solo. A limeira ácida 'Tahiti' é mais precoce, quando comparada com a tangerineira 'Poncã' e a laranjeira 'Serra D'Água'.This study was carried out to evaluate the influence of leaf and soil water potential on flowering of 'Ponkan' mandarin, 'Tahiti' acid lime, and 'Serra d'Água' orange trees under the climate conditions of Viçosa State de Minas Gerais. The experiment was carried out at the Federal University of Viçosa-UFV, in an orchard located in the Fruit crop Sector of the Crop Sciences Department in the period of March to September of 2004. Were evaluate daily during the experimental period the air temperature (ºC and the pluvial precipitation (mm. The soil and the leaf water potential ware

  8. Studies on the Annual Cycle of Endogenous Free Polyamines in Citrus%柑橘不同器官内源游离多胺的年周期变化

    Institute of Scientific and Technical Information of China (English)

    张秋明; 郑玉生; 刘昆玉; 谢深喜; 李大志

    2003-01-01

    对32年生特洛伊(Troyer)枳橙砧(Citrus sinensis×Poncinus trifoliata)华盛顿脐橙[Citrus sinensis (L.)Osbeck]及8年生枳砧[Poncirus trifoliata(L.)Raf.]宫川温州蜜柑(Citrus unshiu Marc.)进行了内源游离多胺年变化规律的研究.结果表明:柑橘不同器官在生长发育的重要时期均伴随着多胺含量的变化,春梢叶片内源多胺含量随叶龄增加而上升,在越冬期急剧增加达全年最高峰,尤以精胺含量增加显著;花期以腐胺含量变化最大,盛花期达高峰,谢花后迅速下降;果实多胺总量变化与横径增长呈平行关系.前期腐胺上升较快,后期主要以亚精胺和精胺增加为主.果皮内源多胺含量大于果肉部分,占果实含量2/3左右.

  9. Application of natural colorants on citrus fruit as alternatives to Citrus Red II

    Science.gov (United States)

    Warm field temperatures can often result in poor peel color of some citrus varieties, especially early in the harvest season. Under these conditions, Florida oranges, temples, tangelos, and K-Early citrus fruit are allowed to be treated with Citrus Red No.2 (CR2) to help produce a more acceptable pe...

  10. CREATION AND HYBRID IDENTIFICATION OF THE SEXUAL PROGENIES USING CITRUS SOMATIC HYBRID AS POLLEN PARENT%柑橘体细胞杂种有性后代的创造及杂种鉴定

    Institute of Scientific and Technical Information of China (English)

    伊华林; 邓秀新

    2005-01-01

    以异源四倍体柑橘体细胞杂种[哈姆林甜橙(Citrus sinensis Osbeck)+粗柠檬(C.jambhiriLuss)]为父本与二倍体单胚类型宜本杂4号[华农本地早橘(C.reticulata Blanco)×宜昌橙(C.ichangensis Swingle)]杂交,采用胚抢救技术获得了110株有性后代植株,通过染色体计数及倍性分析仪鉴定,其中93株植株为3倍体,余下的17株植株为2倍体.RAPD分析表明:获得的有性后代植株均为杂种.

  11. Volatile profiles in cold-pressed peel oil from Korean and Japanese Shiranui (Citrus unshiu Marcov. x C. sinensis Osbeck x C. reticulata Blanco).

    Science.gov (United States)

    Song, Hee Sun; Lan Phi, Nguyen Thi; Park, Yeon-Hee; Sawamura, Masayoshi

    2006-03-01

    A comparison of the volatile profiles between Korean and Japanese Shiranui cold-pressed peel oil was performed by GC and GC-MS. Limonene was the most abundant in the Japanese (91.8%) and Korean (86.4%) oil. Alcohols accounted for 1.8% in the Korean oil, and 0.2% in the Japanese oil, in which the respective linalool levels were 1.2% and 0.1%. The level of aldehydes was also higher in the Korean oil (1.6%) than in the Japanese oil (0.7%). PMID:16556996

  12. INFLUÊNCIA DO ANELAMENTO E ESTIOLAMENTO DE RAMOS NA PROPAGAÇÃO DA LARANJEIRA VALÊNCIA (Citrus sinensis Osbeck ATRAVÉS DE ESTACAS

    Directory of Open Access Journals (Sweden)

    A.M. CASTRO

    1996-05-01

    Full Text Available Objetivando estudar a influência do condicionamento de ramos, através do anelamento e estiolamento parcial, sobre o enraizamento de estacas de laranjeira cv. Valência, conduziu-se o experimento no período de fevereiro a dezembro de 1993. Para o anelamento foi utilizado arame fino de cobre com o qual provocou-se um estrangulamento na base do ramo. No estiolamento, utilizou-se fita preta de polietileno, que foi enrolada na base das brotações. De cada ramo condicionado, obteve-se apenas uma estaca com 15 cm de comprimento, diâmetro médio de 0,5 cm e um par de folhas apicais, cortadas pela metade. O plantio das estacas foi realizado em 12.04.93, em substrato de areia lavada e peneirada. Adotou-se o delineamento inteiramente casualizado, em esquema fatorial 3x3, resultante das combinações entre os períodos de zero, 30 e 60 dias dos fatores anelamento e estiolamento. Verificou-se que não ocorreu enraizamento nas estacas que permaneceram três meses no substrato e variou de 0 até 26,04% para aquelas que permaneceram oito meses. O período de estiolamento influenciou significativamente o percentual de estacas enraizadas.With the aim of studying the effect of branch conditioning through girdling and banding on Valência orange cuttings a rooting trial was carried out from February to December of 1993. The girdling was performed with a thin cooper wire tightly tied at the branch base reaching the log, and the banding with black poliethylene tape, covering the branch at the base of the sprouts. Each conditioned branch gave one cutting of 15 cm length, 0.5 cm diameter and a pair of half cut apical leaves. Cuttings were planted on Dec.04, 93, in washed and sieved sand substrate. The experimental design was completely randomized, with a factorial 3 x 3 from 0, 30 and 60 day periods, girdling and banding. There was no rooting in cuttings that remained three months in the substrate and there was up to 26.04% rooting for those ones that lasted for eight months in the substrate. The banding period significantly affected the cuttings rooting percentage.

  13. Effects of foliar application with compost tea and filtrate biogas slurry liquid on yield and fruit quality of washington navel orange (Citrus sinenesis Osbeck) trees.

    Science.gov (United States)

    Omar, Alaa El-din K; Belal, Elsayed B; El-Abd, Abd El-Naiem A

    2012-07-01

    Sixteen-year-old navel orange trees at a private orchard located in Kafer El-Sheikh Governorate, Egypt, were used in this study. Compost tea (CT) and filtrate biogas slurry liquid (FLB) were applied at two different concentrations (50% and 100%); control trees were sprayed with water Trees treated with CT at 100% were the highest in yield, fruit weight, and vitamin C, whereas the highest percentage of fruit set, fruit number and soluble solid content (SSC), lowest fruit drop, and highest reducing and total sugars were in trees treated with 100% FLB. Concentrations at 50% for both foliar application (CT and FLB) improved yield and fruit characteristics than control treatment. Generally, using a foliar application of compost tea and filtrate biogas slurry liquid at (100%) treatments as food nutrients could be recommended to improve the yield and fruit quality of navel orange fruits under the current study conditions.

  14. Characterization of Aroma Active Compounds in Fruit Juice and Peel Oil of Jinchen Sweet Orange Fruit (Citrus sinensis (L.) Osbeck) by GC-MS and GC-O

    OpenAIRE

    Si Yi Pan; Xiao Lin Yao; Gang Fan; Yun Zhang; Yan Zhang; Bi Jun Xie; Yu Qiao

    2008-01-01

    Gas chromatography-mass spectrometry (GC-MS) and gas chromatographyolfactometry (GC-O) were used to determine the aromatic composition and aroma active compounds of fruit juice and peel oil of Jinchen sweet orange fruit. Totals of 49 and 32 compounds were identified in fruit juice and peel oil, respectively. GC-O was performed to study the aromatic profile of Jinchen fruit juice and peel oil. A total of 41 components appeared to contribute to the aroma of fruit juice and peel oil. Twelve comp...

  15. Characterization of Aroma Active Compounds in Fruit Juice and Peel Oil of Jinchen Sweet Orange Fruit (Citrus sinensis (L. Osbeck by GC-MS and GC-O

    Directory of Open Access Journals (Sweden)

    Si Yi Pan

    2008-06-01

    Full Text Available Gas chromatography-mass spectrometry (GC-MS and gas chromatographyolfactometry (GC-O were used to determine the aromatic composition and aroma active compounds of fruit juice and peel oil of Jinchen sweet orange fruit. Totals of 49 and 32 compounds were identified in fruit juice and peel oil, respectively. GC-O was performed to study the aromatic profile of Jinchen fruit juice and peel oil. A total of 41 components appeared to contribute to the aroma of fruit juice and peel oil. Twelve components were the odorants perceived in both samples. The aromatic compositions of fruit juice were more complex than that of peel oil. Ethyl butanoate, β-myrcene, octanal, linalool, α-pinene, and decanal were found to be responsible for the aromatic notes in fruit juice and peel oil. Nineteen components have been perceived only in the juice and ten compounds were described as aromatic components of only the peel oil by the panelists. These differences lead to the different overall aroma between fruit juice and peel oil.

  16. Physicochemical and functional properties of micronized jincheng orange by-products (Citrus sinensis Osbeck) dietary fiber and its application as a fat replacer in yogurt.

    Science.gov (United States)

    Yi, Tian; Huang, Xingjian; Pan, Siyi; Wang, Lufeng

    2014-08-01

    Orange by-products from juice extraction are generally discarded or used in animal feed due to their low market value. However, orange by-products show potential as dietary fiber (DF) and fat replacers in products such as yogurt. This study assessed the benefits of using orange by-products in DF-enriched materials such as DF powders (OP) and micronized DF with ball-milling (MDF). The study also investigated the effects of adding different levels of OP and MDF on the quality of low-fat yogurt. Results show that MDF showed better physicochemical and functional properties than OP, and that 2% MDF as a fat replacer in yogurt retained most of the textural and sensory properties of full-fat yogurt. Therefore, this study showed that MDF is a promising alternative as a fat replacer in low-fat yogurt, without sacrificing good taste and other qualities of full-fat yogurt.

  17. Comparison of the Composition and Antimicrobial Activities of the Essential Oils of Green Branches and Leaves of Egyptian Navel Orange (Citrus sinensis (L.) Osbeck var. malesy).

    Science.gov (United States)

    Eldahshan, Omayma A; Halim, Ahmed F

    2016-06-01

    The essential oils isolated from the leaves and green branches of the Egyptian navel orange trees were analyzed by GC and GC/MS. A total of 33 and 24 compounds were identified from the oils of the leaves and branches accounting for 96.0% and 97.9%, respectively, of the total detected constituents. The major ones were sabinene (36.5; 33.0%), terpinen-4-ol (8.2; 6.2%), δ-3-carene (7.0; 9.4%), limonene (6.8; 18.7%), trans-ocimene (6.7; 6.1%), and β-myrcene (4.5; 4.4%). The antimicrobial activities of both oils were evaluated using the agar-well diffusion method toward three representatives for each of Gram-positive bacteria, Gram-negative bacteria, and fungi. The oil of leaves was more effective as antimicrobial agent than that of the branches. Streptococcus pyogenes, Staphylococcus aureus, Salmonella typhimurium, and Aspergillus fumigatus were the most sensitive bacteria and fungi by the leaves oil. PMID:26948682

  18. CARACTERIZACIÓN DEL DESARROLLO VEGETATIVO Y SU RELACIÓN CON LA FRUCTIFICACIÓN Y PRODUCCIÓN EN NARANJOS [Citrus sinensis (L.) Osbeck

    OpenAIRE

    Guillermo R. Almenares Garlobo; María del C. Pérez Hernández; Walfredo Torres de la Noval; Mario Varela Nualles; María I. Pavón Rosales

    2015-01-01

    Se caracterizó el desarrollo vegetativo de los cultivares de naranjo `Valencia Late ́ y `Washington Navel`, se determinó la relación del desarrollo vegetativo con la fructificación, la producción y la eficiencia de la fructificación y la producción. Se seleccionó una parcela por cada cultivar y durante cada oleada de brotación, se evaluó el número de hojas por superficie de la copa, las dimensiones de los árboles y la superficie foliar promedio, y se registraron las precipitaciones acumuladas...

  19. Producción y desarrollo de nueve variedades de naranja Citrus sinensis (L. Osbeck en la zona plana del Valle del Cauca

    Directory of Open Access Journals (Sweden)

    Salazar C. Raúl

    1990-12-01

    Full Text Available En árboles de naranja de cinco años injertados sobre mandarina Cleopatra, se evaluaron nueve variedades sembradas en un diseño experimental de bloques completos al azar. El crecimiento de los frutos presentó las tres fases típicas, con duración variable dependiendo de la variedad: lento, crecimiento acelerado y estabilización. Los máximos crecimientos se obtuvieron en las variedades Old Vini, Valencia y Valle Washington. En todas las variedades los sólidos solubles disminuyeron y el porcentaje de acidez aumentó hasta ciertos límites con la edad del fruto. Considerando como momento óptimo para iniciar la cosecha una relación de sólidos solubles: acidez de 9.5:1, la más temprana fue la Valle Washington (7.5 meses, intermedias Old Vini y Australia Navel y la más tardía García Valencia. Todas las variedades presentaron contenidos de jugo mayores del 50 % Y color atractivo de la pulpa. El color más atractivo de la corteza se obtuvo en Parson Brown (97.5 % amarillo, las otras variedades fluctuaron entre 48 y 79%. Todas las variedades tuvieron menos de seis semillas por fruto. Se presentaron dos épocas de cosecha y las variedades con mayor producción: Hamlin, García Valencia y Pineapple tuvieron la mayor variación mensual. La mejor alternativa sembrando 90% de variedades para industria y 10 % para mesa se obtuvo con las variedades Pineapple, Hamlin, Parson Brown, Valle Washington y Old Vini.Five years old orange trees, were grafted on Cleopatra scions of nine cultivars planted in a completely randomized block design were evaluated. Fruit growths presented three typical phases of variable lenght, depending on the cultivar: slow , rapid and stable growth rates. Largest growth were observed in the old Vini, Valencia and Valle Washington cultivars. In all cases total soluble solid contents and acidity increased, up to certain point, with fruit age. Considering that the optimum time to iniciate cropping is when the relationship between solids and acidity is 9.5: 1, the earlient cultivar is Valle Washington (7.5 months while the old Vini and Australian Navel are intermediate and Garcia Valencia is late. All cultivars had solid content above 50% and attractive pulp color. Most attractive rind color was found in the Parson Brown (97.s yellow, the rest fluctuated between 48 and 79%, All cultivars had less than six seeds per fruit, There were two cropping seasons. Largest producers (Hamlin, Garda Valencia and Pineapple had the largest monthy variation, marketing 90 % for industrial purposes and 10% for as fresh product the best cultivars were Pineapple, Hamlin, Parson Brown, Valle Washington and old Vini.

  20. Construction of a Genomic DNA Library of Citrus sinensis Osbeck%脐橙基因组DNA文库的构建

    Institute of Scientific and Technical Information of China (English)

    陈大明; 徐昌杰; 张上隆

    2000-01-01

    研究构建了一个脐橙基因组DNA文库.以'大山岛'脐橙幼叶为试材,CTAB法制备基因组DNA,经CsCl密度梯度离心纯化,获得了大片段(>100 kb)高纯度基因组DNA.以Sau3A I部分酶切,酶切片段3'凹端不完全补平后与LambdaGEM(R)-12 Xho I Half-Site Arms连接,连接产物用Packagene(R) Extract包装,所得噬菌体在KW251寄主上铺平板.文库经过扩增并保存.对该文库特性研究表明,该文库包含了 2.15×106个单个克隆,背景低于100 pfu/μg DNA,插入片段平均大小约为17 kb,证明是一个较为完整的脐橙基因组DNA文库.

  1. Signaling pathways in a Citrus EST database

    OpenAIRE

    Angela Mehta; Marilia Santos Silva; Simone Guidetti-Gonzalez; Helaine Carrer; Marco Aurélio Takita; Natália F. Martins

    2007-01-01

    Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis) was launched in order to sequence Citrus ESTs (expressed sequence tags) from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under differe...

  2. Recent advances in Citrus psorosis virus

    OpenAIRE

    Achachi, Asmae; Ait Barka, Essaïd; Ibriz, Mohammed

    2014-01-01

    Psorosis is a globally devastating disease of citrus caused by an infectious filamentous ophiovirus, Citrus psorosis virus (CPsV), which causes annual losses of about 5 % and a progressive decline of trees by affecting the conductive tissues. The disease can be harboured asymptomatically in many citrus species. In the field, the most characteristic symptoms of the disease in adult trees are bark scaling in the trunk and main branches and also internal staining in the underlying wood. The viru...

  3. Produtividade e qualidade dos frutos da laranjeira 'Pêra' clone IAC em 16 porta-enxertos na região de Bebedouro-SP Yield and fruit quality of 'Pêra' sweet orange clone IAC on 16 rootstocks in Bebedouro region, State of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Eduardo Sanches Stuchi

    2004-08-01

    Full Text Available Avaliou-se a influência de 16 porta-enxertos na produtividade, nas características físicas e químicas (sólidos solúveis totais-°Brix; acidez; ratio; porcentagem de suco; índice tecnológico e tamanho dos frutos dos frutos da laranjeira 'Pêra' [Citrus sinensis (L. Osbeck] e na incidência e severidade da clorose variegada dos citros (CVC. O plantio do experimento foi realizado em julho de 1993, com espaçamento de 6,0 m entre linhas e 3,5 m entre plantas (476 plantas/ha. O experimento foi conduzido sem irrigação. O delineamento experimental foi em blocos ao acaso, duas plantas por parcela, três repetições e 16 tratamentos, constituídos pelas seguintes cultivares porta-enxertos: tangerineira 'Sun Chu Sha Kat' (Citrus reticulata Blanco, tangerineira 'Pectinífera' (C. reticulata, 'Shekwasha' (C. depressa Hayata, tangerineira 'Pectinífera/Shekwasha' (C. depressa Hayata, tangerineira 'Batangas' (C. reticulata, tangerineira 'Oneco' (C. reticulata, citrangor [citrange (Poncirus trifoliata Raf. x C. sinensis x C. sinensis], citrandarin [C.sunki hort. Ex Tanaka x Poncirus trifoliata (L. Raf. cv. English, tangerineira 'Sunki' (C. sunki, tangerineira 'Suen-Kat' (C. sunki, tangerineira 'Nasnaran' (C. amblycarpa Ochse, tangerineira 'Venezuela' (C. reticulata, tangerineira Heen Naran (C. lycopersicaeformis hort. ex Tan. , limoeiro 'Cravo' (C. limonia Osbeck x tangerineira 'Cleópatra' (C. reshni hort ex Tanaka, limoeiro 'Cravo' (C. limonia, tangerineira 'Cleópatra' (C. reshni. A intensidade da clorose variegada dos citros variou em função dos porta-enxertos e não se relacionou com a produção de frutos até a quarta safra. Os porta-enxertos estudados, com exceção da tangerineira Nasnaran, proporcionaram qualidade e produções iniciais de frutos similares aos do limoeiro 'Cravo'.An experiment was set up in July 1993, at Bebedouro city, São Paulo state, Brazil, aiming to evaluate the rootstock influence on the yield and several physical

  4. Green synthesis of gold nanoparticles using Citrus fruits (Citrus limon, Citrus reticulata and Citrus sinensis) aqueous extract and its characterization

    Science.gov (United States)

    Sujitha, Mohanan V.; Kannan, Soundarapandian

    2013-02-01

    This study reports the biological synthesis of gold nanoparticles by the reduction of HAuCl4 by using citrus fruits (Citrus limon, Citrus reticulata and Citrus sinensis) juice extract as the reducing and stabilizing agent. A various shape and size of gold nanoparticles were formed when the ratio of the reactants were altered with respect to 1.0 mM chloroauric acid solution. The gold nanoparticles obtained were characterized by UV-visible spectra, transmission electron microscopy (TEM) and X-ray diffraction (XRD). TEM studies showed the particles to be of various shapes and sizes and particle size ranges from 15 to 80 nm. Selected-area electron diffraction (SAED) pattern confirmed fcc phase and crystallinity of the particles. The X-ray diffraction analysis revealed the distinctive facets (1 1 1, 2 0 0, 2 2 0 and 2 2 2 planes) of gold nanoparticles. Dynamic light scattering (DLS) studies revealed that the average size for colloid gp3 of C. limon, C. reticulata and C. sinensis are 32.2 nm, 43.4 nm and 56.7 nm respectively. The DLS graph showed that the particles size was larger and more polydispersed compared to the one observed by TEM due to the fact that the measured size also includes the bio-organic compounds enveloping the core of the Au NPs. Zeta potential value for gold nanoparticles obtained from colloid gp3 of C. limon, C. reticulata and C. sinensis are -45.9, -37.9 and -31.4 respectively indicating the stability of the synthesized nanoparticles. Herein we propose a novel, previously unexploited method for the biological syntheses of polymorphic gold nanoparticles with potent biological applications.

  5. Comparison of FTIR spectra between Huanglongbing (citrus greening) and other citrus maladies.

    Science.gov (United States)

    Hawkins, Samantha A; Park, Bosoon; Poole, Gavin H; Gottwald, Tim R; Windham, William R; Albano, Joseph; Lawrence, Kurt C

    2010-05-26

    Fourier transform infrared (FTIR) spectroscopy has the ability to quickly identify the presence of specific carbohydrates in plant materials. The presence of the disease huanglongbing (HLB) in the leaves of infected citrus plants has a distinctive spectrum that can be used to distinguish an infected plant from a healthy plant. However, many citrus diseases display similar visible symptoms and are of concern to citrus growers. In this study several citrus diseases (citrus leaf rugose virus, citrus tristeza virus, citrus psorosis virus, and Xanthomonas axonopodis ) and nutrient deficiencies (iron, copper, zinc, manganese, and magnesium) were compared with HLB using FTIR spectroscopy to determine if the spectra alone can be used to identify plants that are infected with HLB instead of another disease. The results indicate that the spectra of some diseases and deficiencies more closely resemble those of apparently healthy plants and some share the carbohydrate transformation that has been seen in the spectra of HLB-infected plants.

  6. Diversidade e dinâmica populacional de ácaros em pomar cítrico Diversity and population dynamics of mites in citrus orchard

    Directory of Open Access Journals (Sweden)

    Marcos Zatti da Silva

    2012-01-01

    Full Text Available As plantas de citros hospedam diversas espécies de ácaros que causam sérios prejuízos à produção e qualidade de frutos no Brasil. Esta pesquisa teve por objetivo estudar a diversidade de ácaros e a dinâmica populacional das principais espécies de ácaros em pomar de citros, Citrus sinensis (L. Osbeck, localizado no município de Descalvado, Estado de São Paulo. Foram realizadas 14 avaliações em um período de 12 meses, de fevereiro de 2007 a fevereiro de 2008. Em cada avaliação, foram coletadas dez folhas e cinco frutos na parte interna de cada uma das 40 plantas demarcadas no pomar. Foram encontradas 34 espécies de ácaros, distribuídas em 17 famílias. As espécies mais abundantes foram: Phyllocoptruta oleivora (Ashmed (Eriophyidae, Panonychus citri (McGregor (Tetranychidae, Brevipalpus phoenicis (Geijskes (Tenuipalpidae e Fungitarsonemus sp. (Tarsonemidae. Entre os ácaros predadores, a família Phytoseiidae foi a de maior riqueza (oito espécies e abundância (1.072 indivíduos. A espécie predominante foi Euseius concordis (Chant, correspondendo a 98,3% dos ácaros desta família. Foram observadas correlações significativas entre densidades populacionais de E. concordis e dos ácaros fitófagos P. citri, B. phoenicis e P. oleivora. Também foram observadas correlações significativas entre densidades populacionais desses ácaros e fatores meteorológicos como temperatura e precipitação pluvial. Os resultados indicam que E. concordis atua como inimigo natural de P. citri, B. phoenicis e P. oleivora no pomar cítrico estudado.The citrus plants are hosts of several species of mites, which cause severe damage to production and quality of fruits in Brazil. The objective of this research was to study the diversity of mites and population dynamics of the main mite species in a citrus orchard, Citrus sinensis (L. Osbeck, in Descalvado County, State of São Paulo, Brazil. Fourteen evaluations were conducted over a period of 12

  7. Histological Characterization of Rind Oil Spotting Disease in Citrus%柑橘油胞凹陷病组织结构特征的研究

    Institute of Scientific and Technical Information of China (English)

    涂俊凡; 吴黎明; 彭抒昂

    2010-01-01

    以4个柑橘品种奉节脐橙(Citrus sinensis Osbeck cv.Fengjie navel orange)、纽荷尔脐橙(C. sinensis Osbeck cv. Newhall navel orange)、红橘(C.reticulata Blanco cv. Red tangerine)和太田椪柑(C. reticulata Blanco cv. Ota ponkan)为试验材料,采用石蜡切片技术,对果皮油胞凹陷病从轻到重的发展过程进行了研究,了解果皮油胞凹陷病各阶段的果皮组织结构特征.结果表明,果皮油胞凹陷病始于黄皮层内4~7层细胞带,受害部位细胞变扁,原生质体收缩位于细胞中央,但表皮细胞仍排列整齐、紧密;受害部位都始于两油胞之间,受害细胞层有多种形式,有的与果皮表面平行,有的为块状,沿着黄皮层延伸,在正常细胞层之间形成明显的受害区;中期细胞变得狭长,原生质体在细胞壁周围缩成薄薄的一层;后期病害到达表皮细胞,造成胞质降解,但油胞仍然完好;末期油胞上部细胞破裂,部分油胞受到伤害,向内凹陷.

  8. 7 CFR 319.56-38 - Citrus from Chile.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Citrus from Chile. 319.56-38 Section 319.56-38... from Chile. Clementines (Citrus reticulata Blanco var. Clementine), mandarins (Citrus reticulata Blanco), and tangerines (Citrus reticulata Blanco) may be imported into the United States from Chile,...

  9. Rapid cyling plant breeding in citrus

    Science.gov (United States)

    Resistance or tolerance to huanglongbing (HLB) and other important traits have been identified in several citrus types and relatives and associated markers should be identified soon. What is urgently needed in addition is an accelerated strategy for citrus variety breeding. Identification and use of...

  10. Cryopreservation and Cryotherapy of Citrus Cultivars

    Science.gov (United States)

    Long-term conservation of Citrus clones can be accomplished by cryopreservation. Shoot tips will survive liquid nitrogen exposure and storage when appropriately desiccated and treated with cryoprotectant solutions. In our research, vegetative Citrus budwood is shipped from Riverside to Fort Collin...

  11. Resistance and tolerance to Huanglongbing in citrus

    Science.gov (United States)

    The Stover-led research team focuses on developing improved citrus scion varieties, through both conventional breeding and transgenics, with special attention to confronting the threat of citrus huanglongbing (HLB). Collaborations are underway with more than 20 research groups around the world to ha...

  12. Saponin-rich fraction from Clematis chinensis Osbeck roots protects rabbit chondrocytes against nitric oxide-induced apoptosis via preventing mitochondria impairment and caspase-3 activation

    OpenAIRE

    Wu, Wenjun; Gao, Xinghua; Xu, Xianxiang; Luo, Yubin; Liu, Mei; Xia, Yufeng; Dai, Yue

    2012-01-01

    Our previous study reported that the saponin-rich fraction from Clematis chinensis Osbeck roots (SFC) could effectively alleviate experimental osteoarthritis induced by monosodium iodoacetate in rats through protecting articular cartilage and inhibiting local inflammation. The present study was performed to investigate the preventive effects of SFC on articular chondrocyte, and explore the underlying mechanisms. Primary rabbit chondrocytes were cultured and exposed to sodium nitroprusside (SN...

  13. Identificação de espécies de citros mediante polimorfismo enzimático Identification of citrus species by means of enzymatic polymorphism

    Directory of Open Access Journals (Sweden)

    Haiko Enok Sawazaki

    1992-01-01

    Full Text Available Estudou-se, mediante polimorfismo enzimático em gel de poliacrilamida, a variabilidade genética das espécies de laranja-doce (Citrus sinensis; laranja-azeda (C. aurantium; tangerinas clementina (C. clementina, sunki (C. sunki, cleópatra (C. reshni e poncã (C. rsticulata; lima-da-pérsia (C. limettioides; limão-galego (C. aurantifolia; limão-cravo (C. limonia e trifoliata (Poncirus trifoliata. Extratos de folhas foram analisados para as isoenzimas de malato deidrogenase (MDH, enzima málica (ME, leucino amino peptidase (LAP, glutamato oxaloacetato transaminase (GOT, fosfoglucoisomerase (PGI, fosfoglucomutase (PGM e isocitrato deidrogenase (IDH. Verificou-se grande variabilidade genética interespecífica, porém nenhuma entre os cultivares de laranja-doce. Foram encontradas algumas aloenzimas, além das referidas pela literatura em gel de amido, como aquelas de uma região próxima ao loco conhecido por Pgm-1, responsável por proteínas monoméricas. Este sistema, denominado PGM, revelou a maior diferenciação entre as espécies, tendo apresentado duas regiões distintas com 9 alelos. No sistema MDH, foram considerados dois locas codificando para proteínas diméricas com 7 alelos; no ME, um loco com 3 alelos; no LAP, possivelmente dois locos responsáveis por proteínas monoméricas com 4 alelos; no GOT, dois focos com 7 alelos; no PGI, um loco com 3 alelos e no IDH, um loco com 4 alelos.The genetic diversity of citrus cultivars was studied by polyacrylamide gel electrophoresis on sweet orange (C. sinensis; tangerines (C. clementine, C. sunki, C. reshni, C. reticulata; Palestine lime (C. Iimettioides; West Indian lime (C. aurantifolia; Rangpur lime (C. limonia, Sour orange (C. aurantium and Poncirus trifoliata. Citrus leaf extracts were analysed for isozymes of malato dehidrogenase (MDH, malic enzyme (ME, leucine aminopeptidase (LAP, glutamate oxaloacetate transaminase (GOT, phosphoglucose isomerase (PGI, phosphoglucose mutase (PGM and

  14. Tolerance of the trifoliate citrus hybrid US-897 (Citrus reticulata Blanco x Poncirus trifoliata L. Raf.) to Huanglongbing

    Science.gov (United States)

    Huanglongbing (HLB) is a destructive disease of citrus in most citrus-producing countries worldwide. The disease, presumably caused by phloem-limited bacteria of the genus Candidatus Liberibacter, affects all known citrus species and citrus relatives with little known resistance. Typical disease s...

  15. Citrus quarantine, sanitary and certification programs in the USA. Prevention of introduction and distribution of citrus diseases

    Science.gov (United States)

    Citrus germplasm originated in Australasia, the Far East, and Africa, thus all citrus grown in the New World was imported. This importation of citrus also resulted in importation of graft transmissible pathogens of citrus, many of which are latent in their original host but can cause epidemics of t...

  16. Produção de frutos e incidência de cancro cítrico em laranjeiras "Monte Parnaso" enxertadas sobre sete porta-enxertos Fruit production and incidence of citrus canker lesions on "Monte Parnaso" navel orange budded on seven rootstocks

    Directory of Open Access Journals (Sweden)

    Bernadete Reis

    2008-06-01

    Full Text Available Porta-enxertos de citros podem exercer influência sobre a produção de frutos e suscetibilidade das plantas ao cancro cítrico. Este trabalho objetivou selecionar porta-enxertos que induzam elevado índice de produtividade e menor suscetibilidade das copas de laranjeiras-de-umbigo "Monte Parnaso"(Citrus sinensis à bactéria Xanthomonas axonopodis pv. citri, causadora do cancro cítrico (CC. O experimento foi desenvolvido em um pomar experimental com 10 anos de idade, plantado em espaçamento de 2,5 x 6,0m, no município de Butiá, no Estado do Rio Grande do Sul. O delineamento experimental foi em blocos ao acaso, com sete tratamentos e quatro repetições, usando duas plantas por parcela. Os tratamentos constaram dos seguintes porta-enxertos: limoeiros "Cravo" (C. limonia e "Volkameriano" (C. volkameriana., trifoliata "Flying Dragon" (Poncirus trifoliata., laranjeira "Caipira" (C. sinensis, citrangeiro "Troyer" (C. sinensis x P. trifoliata, tangerineira "Sunki" (C. sunki. e citrumeleiro "Swingle" (C. paradisi x P. trifoliata. Os parâmetros avaliados foram: incidência de CC em folhas e frutos em toda a planta e em ramos previamente selecionados, em diversas épocas. Verificou-se que as plantas enxertadas sobre citrumeleiro "Swingle" apresentaram o maior índice de produtividade e, à semelhança do "Flying Dragon", a menor incidência de CC, tanto nas folhas como nos frutos. Além disso, verificou-se também que as plantas enxertadas sobre os limoeiros "Cravo" e "Volkameriana", apesar de produzirem as mais elevadas cargas de frutos, foram as mais suscetíveis ao CC.Citrus rootstocks can exert some influences on fruit production and susceptibility of the plants to citrus canker (CC. This research was performed aiming to select rootstocks which induce higher productivity index and lower susceptibility of 'Monte Parnaso' navel orange (Citrus sinensis trees to the bacteria Xanthomonas axonopodis pv. citri. The research was carried out in an

  17. Analysis of Microsatellites in Citrus Unigenes%柑橘EST-SSR分子标记分析

    Institute of Scientific and Technical Information of China (English)

    江东; 钟广炎; 洪棋斌

    2006-01-01

    对来源于甜橙(Citrus sinensis Osbeck)、枳壳(Poncirus trifoliata Raf.)和其他柑橘非冗余EST数据库的38 124条单一基因(Unigene)序列进行了简单重复序列SSRs(Simple Sequence Repeat)搜索,所分析的柑橘非冗余核酸序列总长23.29Mb,从中获得了8 218条SSR,其中包括单碱基重复4 913条(59.8%),2碱基重复1 419条(17.3%),3碱基重复1 709条(20.8%),4碱基重复114条(1.39%),5碱基重复23条(0.28%),6碱基重复40条(0.49%).大约每2.8kb长度的单一基因序列中即存在1个SSR,即平均4.6个单一基因中存在1个SSR.随碱基重复单元(motif)的不同,SSR的最大长度在40-105之间,全部重复序列的平均长度为20.9 bp.各种SSR(1-,2-,3-,4-,5-,6-核苷酸重复)的发生频率在甜橙和枳壳间非常接近.其中单碱基重复序列是最丰富的重复单元,其次为3碱基重复.在所得的SSR的重复单元中,富含A碱基的重复单元的分布占据优势地位,出现的频率与密度均较高,而富含CG碱基的重复单元出现频率和密度较低.用25对EST-SSR引物对6个柑橘品种的多样性进行了PCR检测,结果表明,所有25对引物在6个柑橘品种间均扩增到多样性条带,证实通过柑橘EST数据库的发掘能够高效地筛选到基因水平的SSR标记.%Simple sequence repeats (SSRs) were investigated in the unigene sequences from expressed sequence tags (EST) of sweet orange (Citrussinensis osbeck), trifoliate orange (Poncirus trifoliata Raf) and other citrus species and cultivars. A total of 37 802 citrus unigene sequences corresponding to 23.29 Mb were searched, resulting in the identification of 8 218 SSRs. Among them there were 4 913 (59.8%) mono-, 1 419 (17.3%) di-, 1 709 (20.8%) tri-, 114 (1.39%) tetra-, 23 (0.28%) penta- and 40(0.49%) hexa-nucleotide SSRs. The estimated frequency of SSRs was approximately 1/2.8 kb, which could be extrapolated to 1SSR-containing unigene in 4.6 unigenes. The maximum length of the SSR ranged from 40 to 105 bp

  18. Effects of. gamma. -irradiation on growth and embryogenesis of citrus callus

    Energy Technology Data Exchange (ETDEWEB)

    Nito, Nobumasa; Ling, Jing-Tian; Iwamasa, Masao; Katayama, Yukiyoshi (Saga Univ. (Japan). Faculty of Agriculture)

    1989-09-01

    The embryogenic callus of 'Valencia', orange, 'Yoshida' navel orange (Citrus sinensis (L.) Osbeck), Calamondin (C. madurensis Lour.), Yuzu (C. junos Sieb. ex Tan.) and 'Ishizuka Wase' satsuma (C. unshiu Marc.) were exposed to 1, 5, 10, 20 and 50 kiloroentgen (kR) of gamma irradiation at the dosage of 1 kR/h in Institute of Radiation Breeding, Ministry of Agriculture, Forestry and Fishery, Japan. The irradiation caused a retardation of callus growth. Callus growth was reduced to 50% or less of that in non-irradiated callus, at a dosage of 5 kR in Yuzu, 20 kR in 'Valencia' orange and 'Ishizuka Wase' satsuma, and 50 kR in 'Yoshida' navel orange and Calamondin. The irradiation stimulated embryoid formation from the callus. Optimum irradiation dosages to produce the maximum number of embryoids were 50 kR in 'Valencia' orange, 20 kR in Yuzu, 10 kR in Calamondin and 'Ishizuka Wase' satsuma. 'Yoshida' navel orange produced the maximum number of embryoids at the lowest irradiation of 5 kR. The ratios of embryoid number produced at the optimum irradiation to that at non-irradiation were, 16.4, 8.4, 7.8, 6.4 and 2.7 in Yuzu, 'Ishizuka Wase' satsuma, 'Valencia' orange, 'Yoshida' navel orange and Calamondin, respectively. (author).

  19. Citrus Improvement Using Mutation Techniques

    International Nuclear Information System (INIS)

    Citrus cultivar improvement is hampered by several biological factors inherent to most citrus species. Facultative apomixis, self and cross-incompatibility, long juvenility period, and high heterozygosis are some of the vast arrays of impediments faced by citrus breeders in conventional hybridization. Since oranges and grapefruits are highly polyembryonic, the production of enough numbers of zygotic offspring for selection of superior genotypes of these species is basically impossible; hence, most commercially important cultivars of these species have originated through natural or induced mutation. Star Ruby, a deep-red-fleshed grapefruit, was developed by irradiation of Hudson grapefruit seeds with thermal neutrons. Unlike Hudson, which contains over 50 seeds per fruit, Star Ruby is nearly seedless. Hensz irradiated buds of Ruby Red grapefruit with thermal neutrons and a tree that originated from one of the buds produced fruits three times redder than Ruby Red. It was named A and I-1-48. Ten trees were propagated from A and I -1-48, and out of one of the trees, a budsport mutation was found producing fruits five times redder than Ruby Red. Called Rio Red, it is currently the variety of choice for Texas and is known worldwide for its sweetness, red flesh and beautiful blush. Currently, 37 years after A and I -1-48 was first propagated, the trees are still producing several budsport mutations. So far, in the 2007/2008 season, more than 100 new mutations were obtained from a 100-tree block. In the mandarin group, the existence of several monoembryonic cultivars facilitates conventional breeding, but still, induced mutation is part of most mandarin breeding programmes, and proprietary, new seedless cultivars have been produced in the US, Italy, Israel and elsewhere. Seedless mandarins produced by the University of California Riverside include Dayse, Fairchild, Encore, and Nova. The USDA-ARS, U.S. Horticultural Research Laboratory in Florida released a seedless

  20. Citrus breeding, genetics and genomics in Japan.

    Science.gov (United States)

    Omura, Mitsuo; Shimada, Takehiko

    2016-01-01

    Citrus is one of the most cultivated fruits in the world, and satsuma mandarin (Citrus unshiu Marc.) is a major cultivated citrus in Japan. Many excellent cultivars derived from satsuma mandarin have been released through the improvement of mandarins using a conventional breeding method. The citrus breeding program is a lengthy process owing to the long juvenility, and it is predicted that marker-assisted selection (MAS) will overcome the obstacle and improve the efficiency of conventional breeding methods. To promote citrus molecular breeding in Japan, a genetic mapping was initiated in 1987, and the experimental tools and resources necessary for citrus functional genomics have been developed in relation to the physiological analysis of satsuma mandarin. In this paper, we review the progress of citrus breeding and genome researches in Japan and report the studies on genetic mapping, expression sequence tag cataloguing, and molecular characterization of breeding characteristics, mainly in terms of the metabolism of bio-functional substances as well as factors relating to, for example, fruit quality, disease resistance, polyembryony, and flowering.

  1. Signaling pathways in a Citrus EST database

    Directory of Open Access Journals (Sweden)

    Angela Mehta

    2007-01-01

    Full Text Available Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis was launched in order to sequence Citrus ESTs (expressed sequence tags from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under different development stages (adult vs. juvenile. Several cDNA libraries were constructed and the sequences obtained formed the Citrus ESTs database with almost 200,000 sequences. Searches were performed in the Citrus database to investigate the presence of different signaling pathway components. Several of the genes involved in the signaling of sugar, calcium, cytokinin, plant hormones, inositol phosphate, MAPKinase and COP9 were found in the citrus genome and are discussed in this paper. The results obtained may indicate that similar mechanisms described in other plants, such as Arabidopsis, occur in citrus. Further experimental studies must be conducted in order to understand the different signaling pathways present.

  2. Citrus breeding, genetics and genomics in Japan.

    Science.gov (United States)

    Omura, Mitsuo; Shimada, Takehiko

    2016-01-01

    Citrus is one of the most cultivated fruits in the world, and satsuma mandarin (Citrus unshiu Marc.) is a major cultivated citrus in Japan. Many excellent cultivars derived from satsuma mandarin have been released through the improvement of mandarins using a conventional breeding method. The citrus breeding program is a lengthy process owing to the long juvenility, and it is predicted that marker-assisted selection (MAS) will overcome the obstacle and improve the efficiency of conventional breeding methods. To promote citrus molecular breeding in Japan, a genetic mapping was initiated in 1987, and the experimental tools and resources necessary for citrus functional genomics have been developed in relation to the physiological analysis of satsuma mandarin. In this paper, we review the progress of citrus breeding and genome researches in Japan and report the studies on genetic mapping, expression sequence tag cataloguing, and molecular characterization of breeding characteristics, mainly in terms of the metabolism of bio-functional substances as well as factors relating to, for example, fruit quality, disease resistance, polyembryony, and flowering. PMID:27069387

  3. Yield and quality responses of citrus (Citrus reticulate) and tea (Podocarpus fleuryi Hickel.) to compound fertilizers*

    OpenAIRE

    Wang, Rui; Shi, Xue-gen; Wei, You-zhang; Yang, Xiao-e; Uoti, Juhani

    2006-01-01

    Experiments were carried out with citrus (Citrus reticulate) and tea (Podocarpus fleuryi Hickel.) to study the effects of compound fertilizers on their yields and quality. In the citrus experiment, application of compound fertilizers increased available P, K and Mg contents in soil but decreased alkali-hydrolyzable N contents in soil and N, P and K contents in leaves. In the tea experiment, application of compound fertilizers increased available P, K and Mg contents in soil and N, P, K and Mg...

  4. Characterization of the Asian Citrus Psyllid Transcriptome.

    Science.gov (United States)

    Reese, Justin; Christenson, Matthew K; Leng, Nan; Saha, Surya; Cantarel, Brandi; Lindeberg, Magdalen; Tamborindeguy, Cecilia; Maccarthy, Justin; Weaver, Daniel; Trease, Andrew J; Steven V, Ready; Davis, Vincent M; McCormick, Courtney; Haudenschild, Christian; Han, Shunsheng; Johnson, Shannon L; Shelby, Kent S; Huang, Hong; Bextine, Blake R; Shatters, Robert G; Hall, David G; Davis, Paul H; Hunter, Wayne B

    2014-01-01

    The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is a vector for the causative agents of Huanglongbing, which threatens citrus production worldwide. This study reports and discusses the first D. citri transcriptomes, encompassing the three main life stages of D. citri, egg, nymph and adult. The transcriptomes were annotated using Gene Ontology (GO) and insecticide-related genes within each life stage were identified to aid the development of future D. citri insecticides. Transcriptome assemblies and other sequence data are available for download at the International Asian Citrus Psyllid Genome Consortium website [http://psyllid.org/download] and at NCBI [http://www.ncbi.nlm.nih.gov/bioproject/29447]. PMID:24511328

  5. Characterization of the Asian Citrus Psyllid Transcriptome.

    Science.gov (United States)

    Reese, Justin; Christenson, Matthew K; Leng, Nan; Saha, Surya; Cantarel, Brandi; Lindeberg, Magdalen; Tamborindeguy, Cecilia; Maccarthy, Justin; Weaver, Daniel; Trease, Andrew J; Steven V, Ready; Davis, Vincent M; McCormick, Courtney; Haudenschild, Christian; Han, Shunsheng; Johnson, Shannon L; Shelby, Kent S; Huang, Hong; Bextine, Blake R; Shatters, Robert G; Hall, David G; Davis, Paul H; Hunter, Wayne B

    2014-01-01

    The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is a vector for the causative agents of Huanglongbing, which threatens citrus production worldwide. This study reports and discusses the first D. citri transcriptomes, encompassing the three main life stages of D. citri, egg, nymph and adult. The transcriptomes were annotated using Gene Ontology (GO) and insecticide-related genes within each life stage were identified to aid the development of future D. citri insecticides. Transcriptome assemblies and other sequence data are available for download at the International Asian Citrus Psyllid Genome Consortium website [http://psyllid.org/download] and at NCBI [http://www.ncbi.nlm.nih.gov/bioproject/29447].

  6. Green Synthesis and Biological Activities of Gold Nanoparticles Functionalized with Citrus reticulata, Citrus aurantium, Citrus sinensis and Citrus grandis

    International Nuclear Information System (INIS)

    In the present study, gold nanoparticles (GNPs) were prepared at boiling temperature (90-95 degree C) by treating gold ions with Citrus fruit extracts. The effect of mixing ratios of the reactants and concentration of gold hydrochloride was studied. In the standardization process, 10/sup -3/ M solution of HAuCl/sub 4/.3H/sub 2/O was reacted with fruit extracts for half an hour at 90-95 degree C in different ratios. GNPs were characterized by UV-Vis spectroscopy (UV-Vis) and atomic force microscopy (AFM). Their stability was evaluated against varying pH solutions and volumes of sodium chloride along with metals and antibiotics sensing ability. The gold nanoparticles were tested for antibacterial and antifungal activities against various pathogenic strains. The UV-Vis spectra of gold nanoparticles gave surface plasmon resonance at about 540 nm while the AFM images revealed the particle size within the range of 70-100 nm. GNPs showed remarkable stability in varying pH solutions and salt volumes as well as high detection ability towards cobalt, copper, ceftriaxone and penicillin. Moreover, the GNPs possessed moderate antibacterial and good antifungal activity. These results concluded that the Citrus fruit extracts can be utilized for large scale synthesis of cost-effective nanoparticles which may have compatibility for biomedical and pharmaceutical applications. (author)

  7. Dry matter accumulation in citrus fruit is not limited by transport capacity of the pedicel.

    Science.gov (United States)

    Garcia-Luis, A; Oliveira, M E M; Bordon, Y; Siqueira, D L; Tominaga, S; Guardiola, J L

    2002-12-01

    mass transfer in the phloem but had no influence on fruit growth. These observations demonstrate that the transport capacity in the phloem of the pedicel does not limit fruit growth and, within the limits of our experiments, an increase in demand by the fruit appeared to be matched by an increase in SMT. The dependence of late xylem formation (after the period of fruitlet abscission) on fruitlet growth was demonstrated in Salustiana orange [Citrus sinensis (L.) Osbeck] by means of controlling fruit growth through the manipulation of leaf area. Fruit growth at this time was more closely related to leaf area than to carbohydrate levels, suggesting that it may be limited by current photosynthesis. PMID:12451031

  8. Effectivity of Kaffir lime (Citrus hystrix), Nasnaran Mandarin (Citrus amblycarpa), and Pomelo (Citrus maxima) Leaf Extract Against Aedes aegypti Larvae

    OpenAIRE

    Hebert Adrianto; Subagyo Yotopranoto; Hamidah

    2014-01-01

    The used of chemicals as larvicidal may causing resistance, health problem, and environment problem. Leaf extracts of Citrus as biolarvicidal against Aedes aegypti larvae can be used as an alternative larvicidal. The aims of this study is to find out the species of Citrus in the study sample, which is the most effective as biolarvicidal. This study was conducted using nested experiment design, with six treatments and five replicates. Larvae mortality was observed after 24 and 48 hours. Then, ...

  9. Parentais femininos monoembriônicos na obtenção de porta-enxertos híbridos de citros The use of female monoembryonic parents to obtain citrus rootstocks hybrids

    Directory of Open Access Journals (Sweden)

    Walter dos Santos Soares Filho

    2008-03-01

    Full Text Available Visando a evitar os efeitos da poliembrionia, os quais dificultam a distinção entre indivíduos híbridos e aqueles de origem materna (nucelares. O Programa de Melhoramento Genético de Citros da Embrapa Mandioca e Fruticultura Tropical selecionou híbridos monoembriônicos, com potencial de geração de novos porta-enxertos, e utilizou-os como parentais femininos em polinizações abertas. Esses parentais foram: trifoliados HTR-001; HTR-002; HTR-005; HTR-037; HTR-073 e HTR-135, além de LCR (limoeiro 'Cravo' Citrus limonia x LRF (limoeiro 'Rugoso da Flórida' C. jambhiri - 005. As progênies de meios-irmãos obtidas compreenderam, respectivamente, 364; 55; 12; 58; 51; 551 e 59 seedlings (plantas oriundas de sementes zigóticos. Os híbridos dentro de progênies foram extremamente variáveis quanto à altura da planta e diâmetro do caule, também apresentando diferenças na presença de ramificações, formato, cor e textura do limbo foliar e tamanho de espinhos. A progênie mais vigorosa relacionou-se ao parental HTR-073, dando-se o contrário com aquela relativa ao híbrido LCR x LRF-005. Este trabalho indica que, em citros, a utilização de parentais femininos monoembrônicos em cruzamentos abertos constitui método simples e fácil de obtenção de híbridos.Seeking to avoid the effects of polyembryony, that difficult the identification of hybrids and individuals of maternal origin (nucellar, the Citrus Breeding Program of Embrapa Cassava & Tropical Fruits selected monoembryonic hybrids with potential of generating new rootstocks. Thus, the trifoliate hybrids HTR-001, HTR-002, HTR-005, HTR-037, HTR-073 and HTR-135, besides LCR ('Rangpur' lime Citrus limonia x LRF ('Florida' rough lemon C. jambhiri - 005 were used as female parents in open pollinations design. The half-sibling progenies obtained were, respectively, 364; 55; 12; 58; 51; 551 and 59 zygotic seedlings. The hybrids inside progenies were extremely variable as regards the height

  10. 2004 SWFWMD Citrus County Lidar Survey

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This metadata record describes the ortho & LIDAR mapping of Citrus County, FL. The mapping consists of LIDAR data collection, contour generation, and production...

  11. The aconitate hydratase family from Citrus

    Directory of Open Access Journals (Sweden)

    Cercos Manuel

    2010-10-01

    Full Text Available Abstract Background Research on citrus fruit ripening has received considerable attention because of the importance of citrus fruits for the human diet. Organic acids are among the main determinants of taste and organoleptic quality of fruits and hence the control of fruit acidity loss has a strong economical relevance. In citrus, organic acids accumulate in the juice sac cells of developing fruits and are catabolized thereafter during ripening. Aconitase, that transforms citrate to isocitrate, is the first step of citric acid catabolism and a major component of the citrate utilization machinery. In this work, the citrus aconitase gene family was first characterized and a phylogenetic analysis was then carried out in order to understand the evolutionary history of this family in plants. Gene expression analyses of the citrus aconitase family were subsequently performed in several acidic and acidless genotypes to elucidate their involvement in acid homeostasis. Results Analysis of 460,000 citrus ESTs, followed by sequencing of complete cDNA clones, identified in citrus 3 transcription units coding for putatively active aconitate hydratase proteins, named as CcAco1, CcAco2 and CcAco3. A phylogenetic study carried on the Aco family in 14 plant species, shows the presence of 5 Aco subfamilies, and that the ancestor of monocot and dicot species shared at least one Aco gene. Real-time RT-PCR expression analyses of the three aconitase citrus genes were performed in pulp tissues along fruit development in acidic and acidless citrus varieties such as mandarins, oranges and lemons. While CcAco3 expression was always low, CcAco1 and CcAco2 genes were generally induced during the rapid phase of fruit growth along with the maximum in acidity and the beginning of the acid reduction. Two exceptions to this general pattern were found: 1 Clemenules mandarin failed inducing CcAco2 although acid levels were rapidly reduced; and 2 the acidless "Sucreña" orange

  12. Antihyperlipidemic effects of Citrus sinensis, Citrus paradisi, and their combinations

    Directory of Open Access Journals (Sweden)

    Neelam Mallick

    2016-01-01

    Full Text Available Objective: Hyperlipidemia, extensively contributes in the progression of coronary heart diseases and atherosclerosis, but may be managed through alterations in the nutritional pattern. Several studies show that diet rich in polyphenols and antioxidants have antiatherogenic effects. Citrus sinensis and Citrus paradisi are widely known for health benefits and have found to produce antioxidant, anti-inflammatory, and hypolipidemic effects, hence current research was planned to determine the hypolipidemic effects of C. sinensis and C. paradisi in rats receiving diet rich in cholesterol. Materials and Methods: All rats were divided into 11 groups each comprising 10 animals: Normal control group and hyperlipidemic control. C. sinensis treated three groups, C. paradisi treated three groups, C. sinensis and C. paradisi combination treated two groups, and group treated atorvastatin. All rats in the respective groups were treated orally with sterile water, juices, and standard drug for 8 weeks and lipid profile was estimated at the end of dosing. Results: Cholesterol, triglycerides (TGs, and low-density lipoprotein (LDL were decreased at all the three doses of C. sinensis and C. paradisi but rise in high-density lipoprotein (HDL was only significant at 8 ml/kg, and 0.3 ml/kg, respectively. Animals received the combination doses of C. sinensis and C. paradisi also showed a highly significant fall in cholesterol, LDL, and TGs, however HDL level was significantly elevated by SPJ-2 combination. Conclusion: Results suggest that C. sinensis and C. paradisi possess antihyperlipidemic activity due to phytochemicals and other essential nutrients, hence may serve as cardioprotective by preventing thrombosis.

  13. 刈割、AM真菌和施磷对根箱栽培中柑橘和柱花草生长的影响%Effects of mowing, AM fungal inoculation and phosphorus application on growth of Citrus sinensis and Stylosanthes gracilis in a rhizobox

    Institute of Scientific and Technical Information of China (English)

    姚青; 朱红惠; 胡志群; 陈杰忠

    2004-01-01

    利用根箱栽培模拟果园生草栽培,以柑橘Citrus sinensis (L.) Osbeck.cv.Luogangcheng和柱花草Stylosanthes gracilis H.B.K.cv.Graham来研究生草刈割、AM真菌和施磷在减轻养分竞争上的作用.结果表明,3种措施在不同程度上减轻了柱花草对柑橘生长的抑制效应,增加了柑橘的株高、叶片数和干质量;柱花草刈割的作用最明显,导致柱花草的根冠比急剧降低,表明刈割可能是通过抑制根系生长来减轻养分竞争.

  14. Characterization of the Asian Citrus Psyllid Transcriptome

    OpenAIRE

    Reese, Justin; Christenson, Matthew K.; Leng, Nan; Saha, Surya; Cantarel, Brandi; Lindeberg, Magdalen; Tamborindeguy, Cecilia; MacCarthy, Justin; Weaver, Daniel; Trease, Andrew J.; Ready, Steven V.; Davis, Vincent M.; McCormick, Courtney; Haudenschild, Christian; Han, Shunsheng

    2014-01-01

    The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is a vector for the causative agents of Huanglongbing, which threatens citrus production worldwide. This study reports and discusses the first D. citri transcriptomes, encompassing the three main life stages of D. citri, egg, nymph and adult. The transcriptomes were annotated using Gene Ontology (GO) and insecticide-related genes within each life stage were identified to aid the development of future D. citri insectici...

  15. 腐殖酸结合汞对多年生植物(Citrus Sinensis L.Osbeck) 的生物有效性初探%Bioavailability of Humic-Substance-Bound Mercury to Perennial Plant Citrus Sinensis L. Osbeck

    Institute of Scientific and Technical Information of China (English)

    余贵芬; 吴泓涛; 蒋新; 青长乐

    2006-01-01

    野外采集广柑树及供其生长的土壤,研究多年生植物对土壤Hg的吸收及与土壤理化性质、腐殖酸结合汞(HS-Hg)的关系.结果表明,在酸性土壤环境中,酸性过强,果树吸收Hg量会更低;果实部分Hg含量与土壤的有机质或腐殖酸含量呈现负相关关系;HS-Hg特别是FA-Hg(富啡酸结合汞)组分是果树吸收、积累Hg的重要来源,其与根Hg的相关系数达到0.700*~0.759**,且以表层土壤更能提供有效的HS-Hg.

  16. Evaluation of natural colorants and their application on citrus fruit as alternatives to citrus red II

    Science.gov (United States)

    The poor peel color of some varieties of oranges and the hybrids, especially for early season fruits, is caused by the subtropical climate of Florida, and has resulted in the use of a red dye on the peel to improve fruit appearance and marketability. Citrus Red II (CR2), the commercial citrus color ...

  17. Detection of non-typhoid Salmonella infection by citrus and citrus extracts in Lao PDR

    Institute of Scientific and Technical Information of China (English)

    Yutaka Midorikawa; Satoshi Nakamura; Rattanaphone Phetsouvanh; Manivanh Vongsouvaht; Kaoru Midorikawa

    2010-01-01

    Objective: To know the current state of non-typhoid Salmonella infection in Laos. To examine the usefulness of new screening methods for Salmonella using citrus. Methods: Non-typhoidSalmonella infection of person in Lao PDR was studied in this research (2004-2009). The site was Vientiane capital city in 2004. Research from rural villages locating suburb of Vientiane during 2005-2008 was carried out. Rural villages in Attapu province where ethnic minorities were living was searched for this study in 2009. During this research, to detect Salmonella strain, a new method using citrus and citrus extract named MY phenomenon that observing black ring (MIDO ring) on DHL agar was tried. The slice lemon and lime were used for this trial in 2004. After 2005, disk of ascorbic acid and citric acid were used for the device instead of citrus fruits itself. Results: During this research, 65 of 272 human samples (23.9%) were infected with non-typhoid Salmonella. Conclusions: During this study, the method using citrus and citrus extracts was accepted for the detection of Salmonella. This study shows that with citrus and citrus extract, detection of Salmonella is possible using only DHL media. Results suggest that infectious rate of non-typhoid Salmonella was high.

  18. Interrelations between citrus rust mite, Hirsutella thompsonii and greasy spot on citrus in Surinam

    NARCIS (Netherlands)

    Brussel, van E.W.

    1975-01-01

    Counts of citrus rust mite (Phyllocoptruta oleivora (Ashm.)) on leaves and fruit of citrus rose to a peak in the two dry seasons, the build up taking 4-5 weeks. It then decreased partly through infection by the entomogenous fungus Hirsutellathompsonii Fisher and partly through a decline in feed qual

  19. 78 FR 41259 - Importation of Fresh Citrus Fruit From Uruguay, Including Citrus

    Science.gov (United States)

    2013-07-10

    ... (78 FR 8435-8441, Docket No. APHIS-2011-0060) a proposal \\1\\ to amend the regulations concerning the... Inspection Service 7 CFR Part 319 RIN 0579-AD59 Importation of Fresh Citrus Fruit From Uruguay, Including... the Citrus-related genus Fortunella, from Uruguay into the continental United States. As a...

  20. Phyllosticta citriasiana sp. nov., the cause of Citrus tan spot of Citrus maxima in Asia

    NARCIS (Netherlands)

    Wulandari, N.F.; To-anun, C.; Hyde, K.D.; Duong, L.M.; Gruyter, de J.; Meffert, J.P.; Groenewald, J.Z.; Crous, P.W.

    2009-01-01

    Guignardia citricarpa, the causal agent of Citrus Black Spot, is subject to phytosanitary legislation in the European Union and the U.S.A. This species is frequently confused with G. mangiferae, which is a non-pathogenic, and is commonly isolated as an endophyte from citrus fruits and a wide range o

  1. Phyllosticta citriasiana sp nov., the cause of Citrus tan spot of Citrus maxima in Asia

    NARCIS (Netherlands)

    Wulandari, N.F.; To-anun, C.; Hyde, K.D.; Duong, L.M.; de Gruyter, J.; Meffert, J.P.; Groenewald, J.Z.; Crous, P.W.

    2009-01-01

    Guignardia citricarpa, the causal agent of Citrus Black Spot, is subject to phytosanitary legislation in the European Union and the U.S.A. This species is frequently confused with G. mangiferae, which is a non-pathogenic, and is commonly isolated as an endophyte from citrus fruits and a wide range o

  2. Analysis of the Inheritance and Expression of Antibacterial Peptide Gene Shiva A in Transgenic Citrus(Citrus sinensis) during Its Asexual Propagation%抗菌肽Shiva A基因在转基因柑橘无性繁殖后代中的遗传与表达分析

    Institute of Scientific and Technical Information of China (English)

    许兰珍; 何永睿; 彭爱红; 雷天刚; 刘小丰; 邹修平; 姚利晓; 陈善春

    2013-01-01

    In order to further clarify the genetic stability and the target traits of the foreign gene in the progeny of transgenic citrus,the asexual reproduction plants of transgenic Newhall navel orange(Citrus sinensis Osbeck)containing bivalent antibacterial peptide gene(Shiva A-cecropin B) were studied.In this study,the genetic stability of Shiva A gene in To,T1,T2 and T3 progenies of transgenic citrus varieties were analyzed by PCR,Southern hybridization,Real-time quantitative PCR and greenhouse disease index statistic to Xathomonas axonopodis pv.Citri(Hasse) Dye.The results showed that the antimicrobial peptides Shiva A gene was existed and expressed in To,T1,T2 and T3 plants.This meant target gene could be stably inherited from one generation to another through asexual propagating.There was difference of Shiva A gene copy number between transgenic To and its asexual propagation.Southern bloting analysis showed that To generation had two hybridizations,but its progenies had only one hybridization consistent with To generation.It could be speculated that the T0 generation plant was a transgenic multicellular mixed cytochimera.In addition,Realtime quantitative PCR results showed that,even if Shiva A gene in the To generation genome was double copy numbers,but its expression level was lower than a single copy of the T1,T2 and T3 generation.Therefore,in this study,exogenous gene expression and transgene copy number was a negative correlation.The results of this study provide some basic date and the material for extending the stable phenotype of transgenic citrus strains in carrying out the safety evaluation of transgenic plants.%为进一步明确外源基因在转基因柑橘无性繁殖后代中的遗传稳定性及目标性状表现,本研究以转双价抗菌肽基因(ShivaA-cecropinB)纽荷尔脐橙(Citrus Sinensis Osbeck)无性繁殖后代植株为材料,通过PCR扩增,Southern杂交和实时定量PCR检测,以及温室抗病性评价分析等,对抗菌肽ShivaA基

  3. Dietary citrus pulp reduces lipid oxidation in lamb meat

    OpenAIRE

    Inserra, L.; Priolo, A.; L. Biondi; Lanza, M.; Bognanno, M.; Gravador, R.; Luciano, G.

    2014-01-01

    This study investigated the effect of replacing cereal concentrateswith high levels of dried citrus pulp in the diet on lamb meat oxidative stability. Over 56 days, lambs were fed a barley-based concentrate (Control) or concentrates inwhich 24% and 35% dried citrus pulpwere included to partially replace barley (Citrus 24% and Citrus 35%, respectively). Meat was aged under vacuum for 4 days and subsequently stored aerobically at 4 °C. The Control diet increased the redness, yellowness and satu...

  4. Evaluation of natural colorants and their application on citrus fruit

    Science.gov (United States)

    Warm temperatures can often result in poor peel color of some citrus varieties, especially early in the harvest season. Under these conditions, Florida oranges, temples, tangelos, and K-Early citrus fruit are allowed to be treated with Citrus Red No.2 (CR2) to help produce a more acceptable peel col...

  5. Susceptibility of sixteen citrus genotypes to Candidatus Liberibacter asiaticus

    Science.gov (United States)

    Huanglongbing (HLB) disease is the most serious threat to citrus production worldwide and in the last decade has devastated the Florida citrus industry. HLB is associated with a phloem-limited a-proteobacter Candidatus Liberibacter asiaticus (CLas) and its insect vector the Asian citrus psyllid (Dia...

  6. Trailers transporting oranges to processing plants move Asian citrus psyllids

    Science.gov (United States)

    Huanglongbing (citrus greening) is one of the most serious of citrus diseases. Movement of the disease occurs as a result of natural vector-borne infection and by movement of plant material. We demonstrate here that Diaphorina citri Kuwayama (vector of citrus greening pathogens) can be transported i...

  7. Characterizing the citrus variety Carrizo genome through 454 shotgun sequencing

    Science.gov (United States)

    Citrus production is of global importance both in economic impact and significance to nutrition. The number of natural citrus species appears extremely limited. The genome size is small (haploid approximately 367 Mb), arranged on 18 chromosomes. The citrus variety Carrizo, generated by a ‘Washingt...

  8. First report of citrus leprosis virus nuclear type in Colombia

    Science.gov (United States)

    Citrus leprosis is a difficult viral disease causing significant damage to citrus fruit in South America and Central America. The disease is marked by dramatic lesions on fruit, leaves and stems resulting in unmarketable product. Citrus leprosis virus cytoplasmic types (CiLV-C and CiLV-C2) wer edete...

  9. Thirty years of citrus tristeza virus observations in Peru

    Science.gov (United States)

    The Peruvian citrus industry was devastated by epidemics of Citrus tristeza virus (CTV) decline (CTV-D) on sour orange rootstock between 1950 and 1965 and CTV stem pitting (SP) between 1965 and 1985. CTV-SP debilitates citrus and fruit production regardless of rootstock. Control of CTV-SP by mild st...

  10. Alternation of host plants as a survival mechanism of leafhoppers Dilobopterus costalimai and Oncometopia facialis (Hemiptera: Cicadellidae, vectors of the Citrus Variegated Chlorosis (CVC Alternância de hospedeiros como mecanismo de sobrevivência das cigarrinhas Dilobopterus costalimai e Oncometopia facialis (Hemiptera: Cicadellidae, vetoras da Clorose Variegada dos Citros (CVC

    Directory of Open Access Journals (Sweden)

    José Maria Milanez

    2001-12-01

    Full Text Available Dilobopterus costalimai (Young and Oncometopia facialis (Signoret are two of the most important species of citrus leafhoppers, vectors of bacterium Xylella fastidiosa which causes the Citrus Variegated Chlorosis (CVC disease. To develop a rearing technique for these species under laboratory conditions, the egg laying preference and nymph development were studied in different breeding systems: Rangpur lime (Citrus limonia and "falso boldo" (Vernonia condensata as host plants. Trials were set up in a randomized block design with three treatments (n=8. Females of D. costalimai had particular preference for ovipositing on Rangpur lime leaves while O. facialis females placed a higher number of eggs on "falso boldo", but it did not differ statistically from the Rangpur lime. The nymphal viability of D. costalimai was null in Rangpur lime and 58% in "falso boldo". For O. facialis the nymphal viability was 25 and 78% in Rangpur lime and "falso boldo", respectively. "Falso boldo" is more suitable as a host plant to rear the two species of citrus leafhoppers. The alternation of host plants seems to be an important survival mechanism of the CVC-vector species, as shown in natural conditions.Dilobopterus costalimai (Young e Oncometopia facialis (Signoret são duas das mais importantes espécies de cigarrinhas dos citros, transmissoras da bactéria Xylella fastidiosa causadora da doença conhecida como Clorose Variegada dos Citros (CVC. Com o objetivo de se desenvolver uma técnica de criação destas espécies, em condições de laboratório, estudou-se a preferência por postura e o desenvolvimento ninfal, em diferentes sistemas de criação, tendo como plantas hospedeiras limão cravo (Citrus limonia e falso boldo (Vernonia condensata. O delineamento experimental foi em blocos casualizados com três tratamentos e oito repetições. Fêmeas de D. costalimai tiveram acentuada preferência para ovipositar em folhas de limão cravo, enquanto que fêmeas de O

  11. Evaluation of natural colorants and their application on citrus fruit as alternatives to Citrus Red No. 2

    Science.gov (United States)

    Warm field temperatures can often result in poor peel color of some citrus varieties, especially early in the harvest season. Under these conditions, Florida oranges, temples, tangelos, and K-Early citrus fruit are allowed to be treated with Citrus Red No.2 (CR2) to help produce a more acceptable pe...

  12. Degradation products of citrus volatile organic compounds (VOCs) acting as phagostimulants that increase probing behavior of Asian citrus psyllid

    Science.gov (United States)

    Volatile phytochemicals play a role in orientation by phytophagous insects. We studied antennal and behavioral responses of the Asian citrus psyllid, Diaphorina citri Kuwayama, vector of the citrus greening disease pathogen. Little or no response to citrus leaf volatiles was detected by electroanten...

  13. Alterações das características químicas de três substratos comerciais na produção de mudas cítricas Change of the chemical characteristics of three commercial substrates in the production of citric seedlings

    Directory of Open Access Journals (Sweden)

    Mário Luís Fochesato

    2008-08-01

    Full Text Available Avaliou-se a dinâmica dos nutrientes em três substratos comerciais em que cultivaram-se mudas cítricas, desde o momento da repicagem dos porta-enxertos até a fase final de produção das mesmas. O experimento foi conduzido em ambiente protegido, sendo as mudas cultivadas em citropotes de 4L. O experimento foi desenvolvido na Estação Experimental Agronômica da UFRGS em Eldorado do Sul, RS, no período de junho/2003 a novembro/2004, e obedeceu ao delineamento experimental de blocos casualizados, em esquema fatorial 3 x 3 x 2, sendo: 3 substratos comerciais de cultivo (Comercial 1, 2 e 3 - composto de turfa preta; cascas processadas e enriquecidas, perlita, vermiculita expandida e turfa; e, casca de pinus compostada e vermiculita, respectivamente, 3 porta-enxertos (Trifoliata - Poncirus trifoliata [L.] Raf.; citrangeiro 'C13' - Citrus sinensis [L.] Osbeck x P. trifoliata [L.] Raf.; e limoeiro 'Cravo' - C. limonia Osbeck e 2 variedades copa (laranjeira 'Valência' C. sinensis [L.] Osbeck e a tangerineira 'Montenegrina' - C. deliciosa Tenore. Empregou-se o sistema de irrigação por gotejamento. Avaliou-se a caracterização química dos substratos e conteúdo nutricional foliar e a massa seca da raiz e da parte aérea. Nas condições deste experimento, os substratos apresentam perdas consideráveis de nutrientes, especialmente de fósforo e potássio. No entanto, observa-se que o substrato Comercial 2 apresenta maior aporte de nutrientes, quando comparado com os outros substratos, proporcionando maior acúmulo de massa seca nos porta-enxertos, no momento da enxertia e nas mudas cítricas no final do experimento.The dynamics of nutrients in three commercial substrates were evaluated, from the transplanting until final nursery. The rootstocks were cultivated in 4L containers under greenhouse conditions. The experiment was developed at Estação Experimental Agronômica of UFRGS from June/2003 to November/2004, with the experimental design of

  14. Mudas de laranjeira 'valência' sobre dois porta-enxertos e sob diferentes manejos de adubação 'Valencia' sweet orange nursery trees on two rootstocks under different fertilizer managements

    Directory of Open Access Journals (Sweden)

    Eduardo Augusto Girardi

    2010-09-01

    Full Text Available O manejo da adubação é uma das principais práticas culturais para a produção de mudas cítricas em cultivo protegido. Avaliou-se o efeito de seis tipos de manejo das adubações comercialmente recomendadas na produção de mudas de laranjeira 'Valência' [Citrus sinensis (L. Osbeck] enxertada sobre os porta-enxertos limoeiro 'Cravo' (Citrus limonia Osbeck e citrumeleiro 'Swingle' [Citrus paradisi Macf. x Poncirus trifoliata (L. Raf.]. As avaliações foram conduzidas a partir da transplantação dos porta-enxertos até 180 dias após a enxertia, em viveiro empresarial, em Conchal-SP. Os manejos corresponderam a duas soluções de fertilizantes solúveis aplicadas isoladamente, soluções de fertilizante solúveis associadas a fertilizante de liberação controlada e aplicação exclusiva de fertilizante de liberação controlada. O delineamento experimental adotado foi o fatorial 2 x 6 (porta-enxerto x manejo da adubação, em blocos casualizados, com três repetições e 12 mudas na parcela. O limoeiro 'Cravo' induziu maior crescimento ao enxerto. O crescimento vegetativo das mudas foi similar após o uso de fertilizantes solúveis ou de liberação controlada, apesar da grande variação de quantidades totais de nutrientes fornecidas às plantas. Desta forma, o viveirista poderá optar pelo manejo mais econômico ou prático, conforme as condições locais.The fertilizer program is a major practice for screened citrus nursery tree production. The effect of six fertilizer programs commercially recommended was evaluated on the production of 'Valência' sweet orange [Citrus sinensis (L. Osbeck] nursery trees budded on rootstocks 'Rangpur' lime (Citrus limonia Osbeck and 'Swingle' citrumelo [Citrus paradisi Macf. x Poncirus trifoliata (L. Raf.]. Experimental work was carried out from rootstock transplant until 180 days after budding, in a citrus nursery in Conchal, SP, Brazil. Fertilizer managements consisted of two soluble fertilizers

  15. Exploring the origin of natural citrus hybrid Zigui Tangor%柑橘天然杂种秭归橘橙来源探究

    Institute of Scientific and Technical Information of China (English)

    李俊峰; 向长海; 邓秀新

    2009-01-01

    秭归橘橙为天然柑橘杂种,果实无核、早熟,但其亲本来源未得到深入的分析和验证.为探明秭归橘橙的亲本,应用倍性检测、形态学标记和AFLP、cpSSR、nSSR等分子标记,对其进行遗传鉴定.倍性检测证实秭归橘橙为二倍体,叶形指数、气孔密度和大小、花粉育性和花粉形态分析表明秭归橘橙形态特征上偏向于甜橙,而其花粉染色活力介于甜橙与红橘之间.AFLP聚类分析表明秭归橘橙介于橙和橘之间.cpSSR、nSSR确认秭归橘橙的母本为甜橙(Citrus sinensis[L.] Osbeck),父本为橘类(C.reticulate Blanco).

  16. Noninvasive maturity detection of citrus with machine vision

    Science.gov (United States)

    Ying, Yibin; Xu, Zhenggang; Fu, Xiaping; Liu, Yande

    2004-03-01

    A computer vision system was established to explore a method for citrus maturity detection. The surface color information and the ratio of total soluble solid to titratable acid (TSS/TA) were used as maturity indexes of citrus. The spectral reflectance properties with different color were measured by UV-240 ultraviolet and visible spectrophotometer. The biggest discrepancy of gray levels between citrus pixels and background pixels was in blue component image by image background segmentation. Dynamic threshold method for background segmentation had best result in blue component image. Methods for citrus image color description were studied. The citrus spectral reflectance experiments showed that green surface and saffron surface of citrus were of highest spectral reflectance at the wavelength of 700nm, the difference between them reached to maximum, about 53%, and the image acquired at this wavelength was of more color information for maturity detection. A triple-layer feed forward network was established to map citrus maturity from the hue frequency sequence by the mean of artificial neural network. After training, the network mapper was used to detect the maturity of the test sample set, which was composed of 252 Weizhang citrus with different maturity. The identification accuracy of mature citrus reached 79.1%, that of immature citrus was 63.6%, and the mean identification accuracy was 77.8%. This study suggested that it is feasible to detect citrus maturity non-invasively by using the computer vision system and hue frequency sequence method.

  17. Next generation haplotyping to decipher nuclear genomic interspecific admixture in Citrus species: analysis of chromosome 2

    OpenAIRE

    Curk, Franck; Ancillo, Gema; Garcia-Lor, Andres; Luro, François; Perrier, Xavier; Jacquemoud-Collet, Jean-Pierre; Navarro, Luis; Ollitrault, Patrick

    2014-01-01

    Background The most economically important Citrus species originated by natural interspecific hybridization between four ancestral taxa (Citrus reticulata, Citrus maxima, Citrus medica, and Citrus micrantha) and from limited subsequent interspecific recombination as a result of apomixis and vegetative propagation. Such reticulate evolution coupled with vegetative propagation results in mosaic genomes with large chromosome fragments from the basic taxa in frequent interspecific heterozygosity....

  18. Biogenic emissions from Citrus species in California

    Science.gov (United States)

    Fares, Silvano; Gentner, Drew R.; Park, Jeong-Hoo; Ormeno, Elena; Karlik, John; Goldstein, Allen H.

    2011-09-01

    Biogenic Volatile Organic Compounds (BVOC) emitted from plants are the dominant source of reduced carbon chemicals to the atmosphere and are important precursors to the photochemical production of ozone and secondary organic aerosols. Considering the extensive land used for agriculture, cultivated Citrus plantations may play an important role in the chemistry of the atmosphere especially in regions such as the Central Valley of California. Moreover, the BVOC emissions from Citrus species have not been characterized in detail and more species-specific inputs for regional models of BVOC emissions are needed. In this study, we measured the physiological parameters and emissions of the most relevant BVOC (oxygenated compounds, monoterpenes, and sesquiterpenes) for four predominant Citrus species planted in California ( Citrus sinensis var. 'Parent Navel', Citrus limon var. 'Meyer', Citrus reticulata var. 'W. Murcott' and 'Clementine'). We used two analytical techniques to measure a full range of BVOC emitted: Proton Transfer Reaction Mass Spectrometry (PTR-MS) and gas chromatography with mass spectrometry. Methanol, followed by acetone and acetaldehyde, were the dominant BVOC emitted from lemon and mandarin trees (basal emission rates up to 300 ng(C) g(DW) -1 h -1), while oxygenated monoterpenes, monoterpenes, and sesquiterpenes were the main BVOC emitted from orange trees (basal emission rates up to = 2500 ng(C) g(DW) -1 h -1). Light and temperature-dependent algorithms were better predictors of methanol, acetaldehyde, acetone, isoprene and monoterpenes for all the Citrus species. Whereas, temperature-dependent algorithms were better predictors of oxygenated monoterpenes, and sesquiterpenes. We observed that flowering increased emissions from orange trees by an order of magnitude with the bulk of BVOC emissions being comprised of monoterpenes, sesquiterpenes, and oxygenated monoterpenes. Chemical speciation of BVOC emissions show that the various classes of terpene

  19. ENZYMATIC KINETIC STUDY HYDROLASE FROM CITRUS

    Directory of Open Access Journals (Sweden)

    Israel Hernández

    2015-09-01

    Full Text Available In this paper the degrading activity of enzymes derived from orange peels (Citrus x sinensis, grapefruit (Citrus paradise and pineapple (Ananas comosus on the organic matter in wastewater is evaluated. This activity is measured indirectly by quantifying the biochemical oxygen demand (COD before and after degradation process based on a period of time using the HACH DR / 2010, and then the kinetic study was performed by the differential method and integral with the experimental data, obtaining a reaction order of 1 to pectinase (orange, and order 2 for bromelain (pineapple.

  20. Insecticidal evaluation of essential oils of Citrus sinensis L. (Myrtales: Myrtaceae) against housefly, Musca domestica L. (Diptera: Muscidae).

    Science.gov (United States)

    Kumar, Peeyush; Mishra, Sapna; Malik, Anushree; Satya, Santosh

    2012-05-01

    The housefly, Musca domestica L., is one of the most common insects, associated with vectoring of various etiological agents. In order to search for effective control agent, the essential oil of sweet orange [Citrus sinensis (L.) Osbeck] was evaluated for its insecticidal activity against the larvae and pupae of housefly using contact toxicity and fumigation bioassays. In the contact toxicity assay, lethal concentration, LC(50) of C. sinensis essential oil against housefly larvae, varied between 3.93 and 0.71 μl/cm(2) for different observation days, while lethal time, LT(50), varied between 5.8 to 2.3 days. Mortality of larvae were significant with different concentrations (F = 2.79, df = 4, P housefly larvae, LC(50) of 71.2 and 52.6 μl/l was obtained in 24 and 48 h, respectively. Scanning electron microscopy of oil treated larvae revealed extreme dehydration and surface distortion while control larvae were free from any of the above symptoms and presented smooth surface, conforming effect of essential oil on housefly larvae. Percentage inhibition rate of oil against housefly pupae was 27.3-72.7% for contact toxicity and 46.4-100% for fumigation assay. Compositional analysis of C. sinensis essential oil using gas chromatography/mass spectrometry (GC-MS) revealed D: -limonene (73.24%), α-pinene (5.86%) and myrcene (4.45%) as major components whereas its vapour profile (solid-phase micro extraction-GC/MS) was dominated by D: -limonene at 92.57%. Significant activity of C. sinensis essential oil against larvae and pupae of housefly, pave the way for its use as eco-friendly housefly control measure.

  1. Vigor, produtividade e qualidade de frutos de quatro tangerineiras e híbridos sobre quatro porta-enxertos Vegetative growth, yield and fruit quality of four mandarin and hybrid cultivars on four rootstocks

    Directory of Open Access Journals (Sweden)

    Eduardo Sanches Stuchi

    2008-09-01

    Full Text Available Avaliaram-se o desenvolvimento vegetativo, a produção e a qualidade de frutos das cultivares-copa 'Swatow' (Citrus reticulata Blanco, 'Ellendale' [C. reticulata x C. sinensis (L. Osbeck], 'Fortune' (C. clementina hort. ex Tanaka x C. tangerina hort. ex Tanaka e 'Nova' [C. clementina x (C. paradisi Macfad. x C. reticulata] sobre limoeiro 'Cravo' (C. limonia Osbeck, citrumeleiro 'Swingle' (C. paradisi x P. trifoliata (L. Raf., tangelereiro 'Orlando' (C. reticulata x C. paradisi e tangerineira 'Cleópatra' (C. reshni hort. ex Tanaka, de 2000 a 2006. O experimento foi instalado em Bebedouro-SP, em outubro de 1997. As variáveis analisadas foram: produção anual e produção acumulada de todas as cultivares para todos os anos (2000 a 2006, qualidade de frutos em 2005 e 2006 (massa, sólidos solúveis totais, acidez total, ratio, volume da copa, diâmetro do tronco da copa, eficiência de produção (Kg frutos/m³ copa e índice de alternância de produção. A produção acumulada e a qualidade de frutos das cultivares testadas não foram influenciadas pelos porta-enxertos. O citrumelo 'Swingle' induziu menor porte às plantas das cultivares-copa. O tangelo 'Nova' apresentou características adequadas à comercialização in natura. A tangerineira 'Swatow' produziu frutos de qualidade semelhante aos de tangerineira 'Ponkan'. O tangor 'Ellendale' e a tangerineira 'Fortune' apresentaram produção de frutos baixa e irregular.Plant growth, yield, and fruit quality of 'Swatow' (Citrus reticulata Blanco, 'Ellendale' (C. reticulata x Citrus sinensis L. Osbeck, 'Fortune' (Citrus clementina Hort. ex Tan. x Citrus tangerina Hort. ex Tan. e 'Nova' [(C. clementina x (Citrus paradisi Macf. x C. reticulata] mandarins budded on 'Rangpur' lime (C. limonia Osbeck, 'Swingle' citrumelo (P. trifoliata Raf. x C. paradisi Macf., 'Orlando' tangelo (C. reticulata Blanco x C. paradisi Macf., and 'Cleopatra' mandarin (Citrus reshni Hort. ex Tan. were evaluated, from 2000

  2. Volatile constituents of wild citrus Mangshanyegan (Citrus nobilis Lauriro) peel oil.

    Science.gov (United States)

    Liu, Cuihua; Cheng, Yunjiang; Zhang, Hongyan; Deng, Xiuxin; Chen, Feng; Xu, Juan

    2012-03-14

    Volatiles of a wild mandarin, Mangshanyegan (Citrus nobilis Lauriro), were characterized by GC-MS, and their aroma active compounds were identified by aroma extract dilution analysis (AEDA) and gas chromatography-olfactometry (GC-O). The volatile profile of Mangshanyegan was compared with those of other four citrus species, Kaopan pummelo (Citrus grandis), Eureka lemon (Citrus limon), Huangyanbendizao tangerine (Citrus reticulata), and Seike navel orange (Citrus sinensis). Monoterpene hydrocarbons predominated in Mangshanyegan, in particular d-limonene and β-myrcene, which accounted for 85.75 and 10.89% of total volatiles, respectively. Among the 12 compounds with flavor dilution factors (FD) = 27, 8 oxygenated compounds, including (Z)- and (E)-linalool oxides, were present only in Mangshanyegan. The combined results of GC-O, quantitative analysis, odor activity values (OAVs), and omission tests revealed that β-myrcene and (Z)- and (E)-linalool oxides were the characteristic aroma compounds of Mangshanyegan, contributing to the balsamic and floral notes of its aroma. PMID:22352344

  3. Yield and quality responses of citrus (Citrus reticulate) and tea (Podocarpus fleuryi Hickel.) to compound fertilizers

    Institute of Scientific and Technical Information of China (English)

    WANG Rui; SHI Xue-gen; WEI You-zhang; YANG Xiao-e; UOTI Juhani

    2006-01-01

    Experiments were carried out with citrus (Citrus reticulate) and tea (Podocarpus fleuryi Hickel.) to studythe effects of compound fertilizers on their yieldsand quality. In the citrus experiment, application of compound fertilizers increased available P,K and Mg contents in soil but decreased alkali-hydrolyzable N contents in soil and N, P and K contents in leaves. In the tea experiment, application of compound fertilizers increased available P, K and Mg contents in soil and N, P, K and Mg contents in leaves but decreased alkali-hydrolyzable N in soil compared with the urea treatment. Application of compound fertilizers could improve the quality of citrus and tea, increase their yields and enhance their economical profits significantly. Compared with the control, application of compound fertilizers increased citrus yields by 6.31, 12.94 and 17.69 t/ha, and those of tea by 0.51, 0.86 and 1.30 t/ha, respectively. Correspondingly, profits were increased by 21.4% to 61.1% for citrus and by 10.0% to 15.7% for tea.Optimal rates of compound fertilizers were recommended for both crops.

  4. NaCl胁迫对不同柑橘砧木品种抗氧化系统的影响%Antioxidative responses to NaCl stress in the leaves of different cultivar of citrus rootstocks

    Institute of Scientific and Technical Information of China (English)

    甘海峰; 刘可慧; 傅翠娜; 雷新南; 梅正敏; 肖远辉; 区善汉

    2010-01-01

    采用土培的方式,研究了不同浓度的NaCl(0,50,100,150,250,350 mmol·L~(-1))对岑溪酸橘Citrus reticulata Blanco、龙州土柠檬Citrus limonia Osbeck和临桂砧板柚Citrus grandis Osbeck叶片抗氧化酶(SOD、POD、CAT、APX)活性以及谷胱甘肽(GSH)、丙二醛(MDA)、超氧自由基(O_2~(·-))含量的影响.结果表明,随着NaCl处理浓度的增加,岑溪酸橘、龙州土柠檬和临桂砧板柚3种柑橘叶片中O_2~(·-)均有不同程度的增加,表明在NaCl处理下3种柑橘均受到了不同程度的O_2~(·-)胁迫.NaCl处理对岑溪酸橘和龙州土柠檬SOD、POD活性,临桂砧板柚MDA含量的影响不显著(P>0.05),但引起临桂砧板柚SOD活性、岑溪酸橘和龙州土柠檬APX活性显著增加(P<0.05).在不同浓度的NaCl处理下,3种柑橘叶片的CAT活性均有不同程度的下降,但GSH的含量随着NaCl浓度的增加而增加,在NaCl处理浓度为350 mmol·L~(-1)时,岑溪酸橘、龙州土柠檬、临桂砧板柚GSH含量分别比对照提高了137%、59.1%、90.8%,表明GSH在解毒NaCl胁迫时起重要作用.

  5. Preliminary Studies on Species and Distribution of Citrus Viroids in China

    Institute of Scientific and Technical Information of China (English)

    WANG Xue-feng; ZHOU Chang-yong; TANG Ke-zhi; LAN Jian-qiang; ZHOU Yan; LI Zhon-gan

    2008-01-01

    Citrus viroids are the small but economically important RNA pathogens.For investigating their occurrence and distribution in China,65 viroid samples collected from 8 major citrus cultivated regions were evaluated using one-step or multiplex one-step RT-PCR and biological indexing for specifically detection of Citrus exocortis viroid (CEVd),Citrus bent leaf viroid (CBLVd),Hop stunt viroid (HSVd),Citrus viroid-ⅢI (CVd-Ⅲ) and Citrus viroid-Ⅳ (CVd-Ⅳ).The results showed that there were at least 4 kinds of citrus viroids (CEVd,CBLVd,HSVd,and CVd-Ⅲ) on citrus trees in China.Most of the infected citrus plants harbored more than one viroid species,and two plants were infected with up to 4 citrus viroids.Sweet orange was more frequently infected by viroids than other citrus varieties.It is the preliminary report on the species and distribution of citrus viroids in China.

  6. Digital PCR for detection of citrus pathogens

    Science.gov (United States)

    Citrus trees are often infected with multiple pathogens of economic importance, especially those with insect or mite vectors. Real-time/quantitative PCR (qPCR) has been used for high-throughput detection and relative quantification of pathogens; however, target reference or standards are required. I...

  7. Founder lines for improved citrus biotechnology

    Science.gov (United States)

    This article discusses the research needed to develop the RMCE strategy and molecular assays for site-specific recombinases as tools for genome manipulation. Explanation of genetic engineering used to generate transgenic citrus plants to exhibit a novel phenotype, but not to contain the recombinase...

  8. Ecology of the Asian citrus pysllid

    Science.gov (United States)

    Host selection by psyllids in general appears to involve taste rather than olfaction. Adults are often less discriminating than nymphs. A priori, there is good reason to doubt that Asian citrus psyllid (ACP) uses a long-distance sex pheromone or that ACP orients to host plant volatiles over large (m...

  9. Assessment of Stubborn Disease Incidence in Citrus

    Science.gov (United States)

    Citrus stubborn disease, caused by Spiroplasma Citri, has occured in California for more than 90 years, however, detection methods for estimating disease incidence have not been well developed. Two 8 ha plots in Kern Co. CA were established and sampled in July and August, 2006. Different tissues o...

  10. Assessment of Stubborn Disease Incidence in Citrus

    Science.gov (United States)

    Citrus stubborn disease (CSD) has been a problem in California for over 90 years, yet, methods for rapidly detecting its causal agent, Spiroplasma citri, for use in estimating disease incidence have not been optimized. Two 8 ha blocks within two commercial groves were sampled in July and August, 20...

  11. Citrus tristeza virus-host interactions

    Directory of Open Access Journals (Sweden)

    William O. Dawson

    2013-05-01

    Full Text Available Citrus tristeza virus (CTV is a phloem-limited virus whose natural host range is restricted to citrus and related species. Although the virus has killed millions of trees, almost destroying whole industries, and continually limits production in many citrus growing areas, most isolates are mild or symptomless in most of their host range. There is little understanding of how the virus causes severe disease in some citrus and none in others. Movement and distribution of CTV differs considerably from that of well-studied viruses of herbaceous plants where movement occurs largely through adjacent cells. In contrast, CTV systemically infects plants mainly by long-distance movement with only limited cell-to-cell movement. The virus is transported through sieve elements and occasionally enters an adjacent companion or phloem parenchyma cell where virus replication occurs. In some plants this is followed by cell-to-cell movement into only a small cluster of adjacent cells, while in others there is no cell-to-cell movement. Different proportions of cells adjacent to sieve elements become infected in different plant species. This appears to be related to how well viral gene products interact with specific hosts. CTV has three genes that are not necessary for infection of most of its hosts, but are needed in different combinations for infection of certain citrus species. These genes apparently were acquired by the virus to extend its host range. Some specific viral gene products have been implicated in symptom induction. Remarkably, the deletion of these genes from the virus genome can induce large increases in stem pitting symptoms. The p23 gene, which is a suppressor of RNA silencing and a regulator of viral RNA synthesis, has been shown to be the cause of seedling yellows symptoms in sour orange. Most isolates of CTV in nature are populations of different strains of CTV. The next frontier of CTV biology is the understanding how the virus variants in

  12. Slight Fermentation with Lactobacillus fermentium Improves the Taste (Sugar:Acid Ratio) of Citrus (Citrus reticulata cv. chachiensis) Juice.

    Science.gov (United States)

    Yu, Yuanshan; Xiao, Gengsheng; Xu, Yujuan; Wu, Jijun; Fu, Manqin; Wen, Jing

    2015-11-01

    The aim of this study was to evaluate the hypothesis that fermentation with Lactobacillus fermentium, which can metabolize citric acid, could be applied in improving the taste (sugar:acid ratio) of citrus juice. During fermentation, the strain of L. fermentium can preferentially utilize citric acid of citrus (Citrus reticulata cv. Chachiensis) juice to support the growth without the consumption of sugar. After 6 h of fermentation with L. fermentium at 30 °C, the sugar:acid ratio of citrus juice increased to 22:1 from 12:1, which resulted in that the hedonic scores of sweetness, acidity and overall acceptability of fermented-pasteurized citrus juice were higher than the unfermented-pasteurized citrus juice. Compared with unfermented-pasteurized citrus juice, the ORAC value and total amino acid showed a reduction, and no significant change (P > 0.05) in the L*, a*, b*, total soluble phenolics and ascorbic acid (Vc) content in the fermented-pasteurized citrus juice was observed as compared with unfermented-pasteurized citrus juice. Hence, slight fermentation with L. fermentium can be used for improving the taste (sugar:acid ratio) of citrus juice with the well retaining of quality. PMID:26447635

  13. Slight Fermentation with Lactobacillus fermentium Improves the Taste (Sugar:Acid Ratio) of Citrus (Citrus reticulata cv. chachiensis) Juice.

    Science.gov (United States)

    Yu, Yuanshan; Xiao, Gengsheng; Xu, Yujuan; Wu, Jijun; Fu, Manqin; Wen, Jing

    2015-11-01

    The aim of this study was to evaluate the hypothesis that fermentation with Lactobacillus fermentium, which can metabolize citric acid, could be applied in improving the taste (sugar:acid ratio) of citrus juice. During fermentation, the strain of L. fermentium can preferentially utilize citric acid of citrus (Citrus reticulata cv. Chachiensis) juice to support the growth without the consumption of sugar. After 6 h of fermentation with L. fermentium at 30 °C, the sugar:acid ratio of citrus juice increased to 22:1 from 12:1, which resulted in that the hedonic scores of sweetness, acidity and overall acceptability of fermented-pasteurized citrus juice were higher than the unfermented-pasteurized citrus juice. Compared with unfermented-pasteurized citrus juice, the ORAC value and total amino acid showed a reduction, and no significant change (P > 0.05) in the L*, a*, b*, total soluble phenolics and ascorbic acid (Vc) content in the fermented-pasteurized citrus juice was observed as compared with unfermented-pasteurized citrus juice. Hence, slight fermentation with L. fermentium can be used for improving the taste (sugar:acid ratio) of citrus juice with the well retaining of quality.

  14. INFLUENCE OF SALINITY ON CITRUS: A REVIEW PAPER

    Directory of Open Access Journals (Sweden)

    ADNAN Al-Yassin

    2005-04-01

    Full Text Available Due to the rapid expansion of irrigated agriculture, effi cient use of the limited water resources in arid and semi-arid regions is becoming more and more vital. However, water salinity is a major problem due to its negative infl uence on the yields of many crops. It reduces citrus trees’ growth and causes physiological disorders. Primarily salt-stress lowers net CO2 assimilation, stomatal conductance, and water potential of citrus tree leaves, in addition to accumulation of excessive concentration of Chloride or Sodium in leaves. A great deal of research indicates that citrus have the genetic potential to be salt-sensitive; however inheritance studies in citrus are scarce. In this paper the adverse of effects of salinity on physiological aspects of citrus are reviewed. The review summarizes the prevailing state of knowledge about the responses and tolerance of citrus trees to salinity.

  15. 温度预处理对柑橘果实活性氧代谢相关酶的影响%Effects of Temperature Pre-treatment on the Enzymes Related to the Metabolism of Active Oxygen in Citrus Fruit

    Institute of Scientific and Technical Information of China (English)

    周春华; 胡西琴

    2001-01-01

    以尾张温州蜜柑(Citrus unshiu Marc.)和哈姆林甜橙(Citrus sinensis Osbeck.)为试材,研究了不同温度预处理对果实呼吸速率、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、果皮相对电导率(REC)和木质素含量的影响.结果表明,20℃预处理3 d的果实,在贮藏至100d时,呼吸速率、MDA含量、果皮REC均为最低.20℃预处理果实在贮藏后期SOD与CAT能较好地协同作用清除O-2·,果实受自由基伤害程度最小,果实衰老得以延缓.温度预处理引起的POD活性的上升可能与提高果皮木质素含量、加速受损伤油胞的迅速愈合有关,而贮藏后期POD活性的上升可能与果实的衰老有关.

  16. Research Progress of Medicinal Plant Desmodium styracifolium (Osbeck) Merr.%药用植物广金钱草的研究进展

    Institute of Scientific and Technical Information of China (English)

    蒙爱东

    2008-01-01

    广西和广东常用大宗中药材广金钱草(Desmodium styracifolium (Osbeck) Merr.)是收录品种,生产上以人工栽培为主,主要含有黄酮、生物碱、酚类、鞣质、多糖等化学成分,具有抗泌尿系统结石、改善心血管系统、抗炎和利胆等功效.广金钱草市场需求量大,建议生产上结合经济作物栽培,采取间种或套种的方式,扩大人工栽培规模,在药物研发上,深入研究和开发新的有效化学成分和新的制剂.

  17. Produção e qualidade de frutos de cultivares de laranja-doce no norte do Paraná Fruit production and quality of sweet orange cultivars in northern Paraná state, Brazil

    Directory of Open Access Journals (Sweden)

    Zuleide Hissano Tazima

    2009-06-01

    Full Text Available O objetivo do trabalho foi a caracterização de acessos de laranja-doce [Citrus sinensis (L. Osbeck] do Banco Ativo de Germoplasma de Citros (BAG Citros do IAPAR, em Londrina-PR. Foram estudados os acessos I-02 'Piralima'; I-03 'Barão'; I-11 'Natal', I-16 'Hamlin', I-17 'Seleta-Vermelha', I-60 'Natal', enxertados sobre limão- 'Cravo' (Citrus limonia Osbeck. As plantas foram conduzidas em espaçamento de 7,0 m x 7,0 m e sem irrigação. Os dados de produção (de 1983 a 1997 e as características físico-químicas dos frutos (de 1984 a 2000, como massa (MF, sólidos solúveis totais (SST, acidez titulável total (ATT, ratio (SST/ATT, rendimento em suco (Suco e índice tecnológico (IT foram submetidos à análise de variância, complementada pelo teste de Tukey, a 5% de probabilidade. O acesso I-16 'Hamlin' foi o mais produtivo (218,1 kg por planta e diferiu-se dos demais. Para a massa do fruto, destacaram-se os acessos I-17 'Seleta-Vermelha' (208,8 g e I-11 'Natal' (142,0 g, sem diferença entre si. Com relação às características químicas dos frutos, os acessos apresentaram resultados semelhantes, dentro dos padrões considerados adequados para as cultivares, exceto o acesso I-17, 'Seleta- Vermelha', que teve índice tecnológico menor que 2,0 kg.caixa-1 de SST.The aim of this work was to characterize sweet orange [Citrus sinensis (L. Osbeck] accessions from the IAPAR Active Citrus Germplasm Bank (AGB Citrus in Londrina, Paraná, Brazil. The accessions studied were: I-02 'Piralima', I-03 'Barão', I-11 'Natal', I-16 'Hamlin', I-17 'Seleta Vermelha', I-60 'Natal', I-67 budded on Rangpur lime (Citrus limonia Osbeck. The plants had 7.0 m x 7.0 m spacing, and were carried out without irrigation. Data of yield (from 1983 to 1997 and fruit physical-chemical characteristics (from 1984 to 2000, like fruit mass (FM, total soluble solids (TSS, total titratable acidity (TTA, ratio (TSS/TTA, juice content and technological index (TI were submitted

  18. Progress on Dissecting and Controlling the Citrus Huanglongbing Complex

    OpenAIRE

    Duan, Yongping; Zhou, Lijuan; Zhang, Muqing; Benyon, Lesley; Armstrong-Vahling, Cheryl; Hoffman, Michele; Hao, Guixia; Zou, Huasong; Doud, Melissa; Ding, Fang; Morgan, Kent

    2014-01-01

    Citrus huanglongbing (HLB) is a century-old and emerging disease that impedes citrus production worldwide. ‘Candidatus Liberibacter asiaticus’ (Las) is the globally prevalent species of HLB bacteria. Here we describe our molecular characterizations of Las, and our newly-developed control methods for citrus HLB. From a genomics standpoint, we revealed Las has a significantly reduced genome (1.26Mb) and unique features adapted to its intracellular life style.  Although the genome is small, Las ...

  19. Citrus Genetic Resources Grown on the Ryukyu Islands, Japan

    OpenAIRE

    Yamamoto, Masashi; ヤマモト, マサシ; 山本, 雅史

    2014-01-01

    The Ryukyu Islands are located southwest part of Japan. Various local citrus are grown in this subtropical region. Since there are large geographical and climatic differences between the Ryukyu Islands and the main islands (Honshu, Kyushu, and Shikoku) of Japan, there are unique local citrus genetic resources on the Ryukyu Islands. Shiikuwasha (Citrus depressa) is an indigenous mandarin species in this region. This species is clearly distinguished from the mandarin grown in China and India ba...

  20. Synergy and Other Interactions between Polymethoxyflavones from Citrus Byproducts

    OpenAIRE

    Benito F. García; Ascensión Torres; Francisco A. Macías

    2015-01-01

    The citrus by-products released from citrus processing plants may contain high levels of potentially bioactive compounds such as flavonoids, which are a widely distributed group of polyphenolic compounds with health-related properties based on their antioxidant activity. In the study reported here, the potential bioactivities and antioxidant activities of extracts, fractions and compounds from citrus by-products were evaluated along with the chemical interactions of binary mixtures of compoun...

  1. Production of aromatic acids during anaerobic digestion of citrus peel

    Energy Technology Data Exchange (ETDEWEB)

    Lane, A.G.

    1980-06-01

    Commercially prepared citrus oils, distilled citrus oils, limonene and the non-volatile fraction of lemon oils were all found to be toxic to the anaerobic digestion process for conversion of citrus waste to methane. Toxicity was characterised by appearance of benzoic, phenylacetic and phenylpropionic acids in the digestion liquors, though these acids were not in themselves toxic. The bulk of the phenylpropionic acid was derived from flavonoids.

  2. Resistance to Gas Diffusion in Internal Tissues of Citrus Fruit

    OpenAIRE

    Dirpan, Andi

    2015-01-01

    Japan is a major citrus fruit-producing country, and Ehime Prefecture is one of the main citrus fruit producing regions in Japan. As many as 20 major citrus varieties are cultivated in Ehime. To further develop postharvest technology for quality preservation such as modified atmosphere packaging (MAP) and controlled atmosphere (CA) storage, creating an optimum gas concentration around fruit and vegetables have to be precisely achieved. However, the external atmosphere of fresh products in MAP...

  3. Mechanisms of sexual polyploidization and inheritance in triploid citrus populations

    OpenAIRE

    CUENCA IBÁÑEZ, JOSÉ

    2013-01-01

    Citrus is the main fruit crop in the world and Spain is the 6th producer and the major exporter for the fresh fruit market. Seedlessness is one of the most important fruit quality traits for this market since consumers do not accept seedy fruits. Recovery of triploid hybrids has become an important breeding strategy to develop new seedless citrus varieties and several of them have been already released from citrus breeding programs worldwide. Despite the undisputable importance of polyploidy ...

  4. Discrimination of Citrus reticulata Blanco and Citrus reticulata 'Chachi' by gas chromatograph-mass spectrometry based metabolomics approach.

    Science.gov (United States)

    Duan, Li; Guo, Long; Dou, Li-Li; Zhou, Chang-Lin; Xu, Feng-Guo; Zheng, Guo-Dong; Li, Ping; Liu, E-Hu

    2016-12-01

    Citri Reticulatae Pericarpium, mainly including the pericarp of Citrus reticulata Blanco and the pericarp of Citrus reticulata 'Chachi', has been consumed daily as food and dietary supplement for centuries. In this study, GC-MS based metabolomics was employed to compare comprehensively the volatile constituents in Citrus reticulata Blanco and Citrus reticulata 'Chachi'. Principal component analysis and orthogonal partial least squares discrimination analysis indicated that samples could be distinguished effectively from one another. Fifteen metabolites were finally identified for use as chemical markers in discrimination of Citri Reticulatae Pericarpium samples. The antimicrobial activity against Gram-negative and Gram-positive bacteria of the volatile oil from Citrus reticulata Blanco and Citrus reticulata 'Chachi' was investigated preliminarily. PMID:27374515

  5. Enumerative and binomial sampling plans for citrus mealybug (Homoptera: pseudococcidae) in citrus groves.

    Science.gov (United States)

    Martínez-Ferrer, María Teresa; Ripollés, José Luís; Garcia-Marí, Ferran

    2006-06-01

    The spatial distribution of the citrus mealybug, Planococcus citri (Risso) (Homoptera: Pseudococcidae), was studied in citrus groves in northeastern Spain. Constant precision sampling plans were designed for all developmental stages of citrus mealybug under the fruit calyx, for late stages on fruit, and for females on trunks and main branches; more than 66, 286, and 101 data sets, respectively, were collected from nine commercial fields during 1992-1998. Dispersion parameters were determined using Taylor's power law, giving aggregated spatial patterns for citrus mealybug populations in three locations of the tree sampled. A significant relationship between the number of insects per organ and the percentage of occupied organs was established using either Wilson and Room's binomial model or Kono and Sugino's empirical formula. Constant precision (E = 0.25) sampling plans (i.e., enumerative plans) for estimating mean densities were developed using Green's equation and the two binomial models. For making management decisions, enumerative counts may be less labor-intensive than binomial sampling. Therefore, we recommend enumerative sampling plans for the use in an integrated pest management program in citrus. Required sample sizes for the range of population densities near current management thresholds, in the three plant locations calyx, fruit, and trunk were 50, 110-330, and 30, respectively. Binomial sampling, especially the empirical model, required a higher sample size to achieve equivalent levels of precision.

  6. Phylogenetic Analysis of Citrus tristeza virus Isolates of Wild Type Citrus in China

    Institute of Scientific and Technical Information of China (English)

    YI Long; ZHOU Chang-yong

    2014-01-01

    The genetic variation and phylogenetic relationships of Citrus tristeza virus (CTV) isolates collected from Chinese wild type citrus were analyzed by comparing the sequences of nine genomic regions (p23, p20, p13, p18, p25, p27, POL, HEL and k17) with the CTV isolates of cultivated citrus from different countries. The results showed that the divergence pattern of genomic RNA of the CTV isolates from wild type citrus was similar to that of other isolates from cultivated citrus, the 3´ proximal region was relatively conserved, and the 5´ proximal region had greater variability. The nine genomic regions of CTV isolates analyzed were found to have been under purifying selection in the evolution process. Phylogenetic analysis showed that the eleven Chinese wild CTV isolates were located at different clades and did not relfect their geographical origins, suggesting genetic diversity among the Chinese wild CTV populations. These results will aid in the understanding of molecular evolution of the Chinese CTV populations.

  7. Physicochemical Characteristics of Citrus Seed Oils from Kerman, Iran

    OpenAIRE

    Mohammad Reazai; Issa Mohammadpourfard; Shahrokh Nazmara; Mahdi Jahanbakhsh; Leila Shiri

    2014-01-01

    Recently, there has been a great deal of attention on usage, byproducts, and wastes of the food industry. There have been many studies on the properties of citrus seeds and extracted oil from citrus grown in Kerman, Iran. The rate of oil content of citrus seeds varies between 33.4% and 41.9%. Linoleic acid (33.2% to 36.3%) is the key fatty acid found in citrus seeds oil and oleic (24.8% to 29.3%) and palmitic acids (23.5% to 29.4%) are the next main fatty acids, respectively. There are also o...

  8. Citrus Flavonoids as Regulators of Lipoprotein Metabolism and Atherosclerosis.

    Science.gov (United States)

    Mulvihill, Erin E; Burke, Amy C; Huff, Murray W

    2016-07-17

    Citrus flavonoids are polyphenolic compounds with significant biological properties. This review summarizes recent advances in understanding the ability of citrus flavonoids to modulate lipid metabolism, other metabolic parameters related to the metabolic syndrome, and atherosclerosis. Citrus flavonoids, including naringenin, hesperitin, nobiletin, and tangeretin, have emerged as potential therapeutics for the treatment of metabolic dysregulation. Epidemiological studies reveal an association between the intake of citrus flavonoid-containing foods and a decreased incidence of cardiovascular disease. Studies in cell culture and animal models, as well as a limited number of clinical studies, reveal the lipid-lowering, insulin-sensitizing, antihypertensive, and anti-inflammatory properties of citrus flavonoids. In animal models, supplementation of rodent diets with citrus flavonoids prevents hepatic steatosis, dyslipidemia, and insulin resistance primarily through inhibition of hepatic fatty acid synthesis and increased fatty acid oxidation. Citrus flavonoids blunt the inflammatory response in metabolically important tissues including liver, adipose, kidney, and the aorta. The mechanisms underlying flavonoid-induced metabolic regulation have not been completely established, although several potential targets have been identified. In mouse models, citrus flavonoids show marked suppression of atherogenesis through improved metabolic parameters as well as through direct impact on the vessel wall. Recent studies support a role for citrus flavonoids in the treatment of dyslipidemia, insulin resistance, hepatic steatosis, obesity, and atherosclerosis. Larger human studies examining dose, bioavailability, efficacy, and safety are required to promote the development of these promising therapeutic agents. PMID:27146015

  9. Macronutrientes em mudas de citros cultivadas em vasos em resposta à adubação NPK Macronutrients in citrus nursery trees grown in pots in response to NPK fertilization

    Directory of Open Access Journals (Sweden)

    Alberto Carlos de Campos Bernardi

    2000-12-01

    Full Text Available Mudas de laranjeira 'Valência' (Citrus sinensis sobre o porta-enxerto de limoeiro 'Cravo' (C. limonia foram cultivadas em vasos com substrato de casca de Pinus, vermiculita e perlita com o objetivo de avaliar-se os efeitos do fornecimento de N, P e K sobre os teores de macronutrientes do porta-enxerto e das mudas. Utilizou-se um esquema de fatorial fracionário (1/55³ com um delineamento em blocos ao acaso. Os tratamentos consistiram em 5 doses (em g por planta de N: 1,25; 6,25; 11,25; 16,25; 21,25; 5 doses de K: 0,42; 3,75; 6,22; 9,34; 12,45; e 5 doses de P: 0,19; 0,89; 1,59; 2,29; 2,99. O N e K foram fornecidos semanalmente, sendo 37,5% da dose para o porta-enxerto. O P foi fornecido totalmente no plantio. Determinaram-se os teores totais de N, P e K, Ca, Mg e S nas folhas novas e velhas, raízes e caule e a extração de N, P e K. Os teores de N relacionaram-se diretamente e os de P e K inversamente com a adubação nitrogenada. Os teores de Ca, Mg e S relacionaram-se positivamente até as doses intermediárias de N utilizadas. Houve efeito inibitório do fertilizante potássico sobre a absorção de Ca e Mg. A acumulação de NPK pelos porta-enxertos foi em torno de 30% pelas raízes e 70% pela parte aérea.'Valencia' sweet orange nursery trees (Citrus sinensis budded on Rangpur lime rootstocks (C. limonia were grown in containers using pine bark, vermiculite and perlite as substracts with the objective of evaluating the NPK fertilization effect on macronutrient levels. The experiment was a complete (1/5 5³ factorial randomized block design. Treatments comprised five concentrations in g per plant of N: 1.25; 6.25; 11.25; 16.25; 21.25; five concnetrations of K: 0.42; 3.75; 6.22; 9.34; 12.45; and five concentration of P: 0.19; 0.89; 1.59; 2.29; 2.99. Nitrogen and K were applieded weekly, and 37.5% of the total fertilization was applied to rootstocks. All P was supplied at planting. Young and old leaves, root and stem total nutrient

  10. Desenvolvimento de mudas de citros cultivadas em vaso em resposta à adubação NPK Development of citrus nursery trees grown in pots in response to NPK fertilization

    Directory of Open Access Journals (Sweden)

    Alberto Carlos de Campos Bernardi

    2000-12-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da adubação NPK sobre o desenvolvimento de mudas de laranjeira 'Valência' (Citrus sinensis sobre o porta-enxerto de limoeiro 'Cravo' (C. limonia em vasos com substrato de casca de Pinus, vermiculita e perlita. Utilizou-se um esquema de fatorial fracionário (1/55³ com um delineamento em blocos ao acaso. Os tratamentos consistiram em 5 doses (em g por planta de N: 1,25; 6,25; 11,25; 16,25; 21,25; 5 doses de K: 0,42; 3,75; 6,22; 9,34; 12,45; e 5 doses de P: 0,19; 0,89; 1,59; 2,29; 2,99. O N e o K foram fornecidos semanalmente, sendo 37,5% da dose para o porta-enxerto. O P foi fornecido totalmente no plantio. Avaliaram-se a produção de material seco das folhas, raízes, caule, parte aérea e total; área foliar, relação raízes/parte aérea; altura de plantas; volume radicular, relação área foliar/material seco para os porta-enxertos e as mudas, e o diâmetro do caule para os porta-enxertos. Funções de resposta foram ajustadas e os níveis de nutrientes foram calculados. Os resultados mostraram que as doses de fertilizantes que levaram às melhores respostas para produção de mudas de laranjeira 'Valência'/'Cravo' sobre o porta-enxertos de limoeiro 'Cravo' foram: N, 9,85; P, 2,86; K, 7,99 g por plantas.The objective of this research was to evaluate the NPK fertilizer effect on 'Valencia' sweet orange nursery tree (Citrus sinensis development, budded on Rangpur lime rootstocks (C. limonia in a protected environment using containers with Pinus bark, vermiculite and perlite substrates. The experiment consisted of a complete (1/5 5³ factorial randomized block design. Treatments comprised five concentrations in g per plant of N: 1.25; 6.25; 11.25; 16.25; 21.25; five concnetrations of K: 0.42; 3.75; 6.22; 9.34; 12.45; and five concentration of P: 0.19; 0.89; 1.59; 2.29; 2.99. Nitrogen and K were applieded weekly, and 37.5% of the total fertilization was applied to rootstocks. All P was

  11. Monitoring the viability of citrus rootstocks seeds stored under refrigeration Monitoramento da viabilidade de sementes porta-enxertos de citros armazenados em câmara fria

    Directory of Open Access Journals (Sweden)

    Sérgio Alves de Carvalho

    2013-03-01

    Full Text Available The citrus nursery tree is produced through the bud grafting process, in which rootstock is usually grown from seed germination. The objective of this research was to evaluate, in two dissimilar environmental conditions, the viability and polyembryony expression of five citrus rootstocks seeds stored in different periods under refrigeration. The rootstock varieties evaluated were: Rangpur lime (Citrus limonia Osb. cv. Limeira, Trifoliate orange (Poncirus trifoliata Raf. cv. Limeira, Citrumelo (P. trifoliata x C. paradisi Macf. cv. Swingle, Sunki mandarin (C. sunki Hort. ex Tanaka and Volkamer lemon (C. volkameriana Ten. & Pasq. cv. Catania 2. The experimental design was the randomized blocks in a 11 x 5 x 2 factorial scheme, evaluating from time zero to the tenth month of storage, the five varieties of rootstock in two environments: germination and growth B.O.D type chamber (Biological Oxygen Demand - Eletrolab Brand Model FC 122 at 25 °C; and greenhouse seedbed with partial temperature control (22 °C to 36 °C and humidity control (75-85%. The plot had 24 seeds in four replicates, using trays with substrate in greenhouse and Petri dishes with filter paper in B.O.D. chamber. The seed germination rate and polyembryony expression were evaluated monthly. It was concluded that Trifoliate and Citrumelo Swingle seeds can be stored for up to seven months, while Volkamer lemon, Rangpur lime and Sunki seeds can be stored for up to ten months. The polyembryony expression rate was slightly higher when measured in greenhouse than in B.O.D. chamber and remained stable in both environments until the seventh month, from which dropped sharply. Citrumelo Swingle seeds expressed the highest polyembryony rate (18.8%, followed by Rangpur lime and Volkamer lemon (average value of 13.7%, Sunki (9.4% and Trifoliate (3.2%. Despite some differences among varieties, the viability of rootstock stored seeds can be monitored either in the greenhouse or in B

  12. Subacute toxicity assessment of carotenoids extracted from citrus peel (Nanfengmiju, Citrus reticulata Blanco) in rats.

    Science.gov (United States)

    Xue, Feng; Li, Chen; Pan, Siyi

    2012-02-01

    The mixture of carotenoids extracted from citrus peel (Nanfengmiju, Citrus reticulata Blanco) was tested for subacute oral toxicity. In this study, dose levels of 0, 200, 500 and 2000 mg/kg body weight/day were administered by gavage to 10 Wistar rats/sex/group for 28 days. No statistically significant, dose-related effect on food consumption, food efficiency, body weight gain, clinical signs or ophthalmoscopic parameters was observed in any treatment group. Urinalysis, hematological, blood coagulation and serum biochemical examination as well as necropsy or histopathology showed that no observed adverse effect was found. These findings suggested that the No-Observed-Adverse-Effect Level for the mixture of carotenoids extracted from citrus peel was at least 2000 mg/kg body weight/day. PMID:22197624

  13. EFEITO DA COMPOSIÇÃO DO SUBSTRATO NA FORMAÇÃO DE MUDAS DE LARANJEIRA `PERA'

    Directory of Open Access Journals (Sweden)

    F.A.A. MOURÃO FILHO

    1998-01-01

    Full Text Available O efeito de cinco composições de substratos na produção de mudas de laranjeira `Pêra' (Citrus sinensis, L. Osbeck foi estudado sobre três porta-enxertos (limoeiro `Cravo', Citrus limonia, L. Osbeck; tangerineira `Cleópatra', Citrus reticulata, Blanco; e citrumeleiro `Swingle', Citrus paradisi x Poncirus trifoliata. O experimento obedeceu ao delineamento estatístico de blocos aleatorizados em esquema fatorial 5 x 3 (substratos x porta-enxertos. As composições de misturas utilizadas como substrato e respectivas combinações volumétricas foram: 1 Terra (100%; 2 Terra (33% + areia fina (33% + esterco bovino curtido (33%; 3 Terra (33% + vermiculita (33% + esterco bovino curtido (33%; 4 Terra (33% + raspas de madeira (33% + esterco bovino curtido (33%; 5 Terra (25% + raspas de madeira (25% + areia fina (25% + esterco bovino curtido (25%. A análise dos resultados revelou que plantas de limoeiro `Cravo' e citrumeleiro `Swingle' apresentaram maior desenvolvimento do que as de tangerineira `Cleópatra'. A mistura de materiais com solo mostrou-se vantajosa e induziu à formação de plantas mais desenvolvidas do que aquelas em substrato constituído de terra exclusivamente. A composição do substrato influenciou diferentemente no desenvolvimento de cada porta-enxerto, mas de uma forma geral, composições contento 1/3 de volume de solo e 1/3 de volume de esterco bovino proporcionaram bons resultados para os três porta-enxertos estudados.To verify the effect of five substrate mixtures on the development of young sweet orange trees (Citrus sinensis cv. `Pera', this study used the rootstocks Ranpur lime (Citrus limonia L. Osbeck, Cleopatra mandarin (Citrus reticulata, Blanco, and Swingle citrumelo (Citrus paradisi x Poncirus trifoliata. The experimental design was in randomized blocks, 5 x 3 factorial (substrates x rootstocks. The different materials and their proportion studied were, respectively: 1 Soil (100%; 2 Soil (33% + fine sand (33

  14. New depside from Citrus reticulata Blanco.

    Science.gov (United States)

    Phetkul, Uraiwan; Phongpaichit, Souwalak; Watanapokasin, Ramida; Mahabusarakam, Wilawan

    2014-01-01

    A new depside, named depcitrus A (1), and 31 known compounds were isolated from the peels, leaves and branch barks of Citrus reticulata Blanco. Methylation of the high polarity fractions from the branch barks and peels gave one new methylated compound named depcitrus B (14) and five known compounds. Their structures were established based on spectroscopic evidence. The antioxidant, antimicrobial and cytotoxic activities of some pure compounds were evaluated. PMID:24635118

  15. Citrus phytophthora diseases: Management challenges and successes

    OpenAIRE

    Graham, J; Feichtenberger, E.

    2015-01-01

    Phytophthora spp. are present in nearly all citrus groves in Florida and Brazil and phytophthora-induced diseases, especially foot and root rot, have the potential to cause economically important crop losses. Disease-related losses due to root rot are difficult to estimate because fibrous root damage and yield loss are not always directly proportional. Challenges from phytophthora diseases have been addressed in both countries by enacting phytosanitary requirements for production of pathogen-...

  16. Chemistry and Pharmacology of Citrus sinensis

    OpenAIRE

    Juan Manuel J. Favela-Hernández; Omar González-Santiago; Mónica A. Ramírez-Cabrera; Patricia C. Esquivel-Ferriño; María del Rayo Camacho-Corona

    2016-01-01

    Presently the search for new drugs from natural resources is of growing interest to the pharmaceutical industry. Natural products have been the source of new drugs since ancient times. Plants are a good source of secondary metabolites which have been found to have beneficial properties. The present study is a review of the chemistry and pharmacology of Citrus sinensis. This review reveals the therapeutic potential of C. sinensis as a source of natural compounds with important activities that ...

  17. Polyembryony in non-apomictic citrus genotypes

    Science.gov (United States)

    Aleza, Pablo; Juárez, José; Ollitrault, Patrick; Navarro, Luis

    2010-01-01

    Background and Aims Adventitious embryony from nucellar cells is the mechanism leading to apomixis in Citrus sp. However, singular cases of polyembryony have been reported in non-apomictic genotypes as a consequence of 2x × 4x hybridizations and in vitro culture of isolated nucelli. The origin of the plants arising from the aforementioned processes remains unclear. Methods The genetic structure (ploidy and allelic constitution with microsatellite markers) of plants obtained from polyembryonic seeds arising from 2x × 4x sexual hybridizations and those regenerated from nucellus culture in vitro was systematically analysed in different non-apomictic citrus genotypes. Histological studies were also conducted to try to identify the initiation process underlying polyembryony. Key Results All plants obtained from the same undeveloped seed in 2x × 4x hybridizations resulted from cleavage of the original zygotic embryo. Also, the plants obtained from in vitro nucellus culture were recovered by somatic embryogenesis from cells that shared the same genotype as the zygotic embryos of the same seed. Conclusions It appears that in non-apomictic citrus genotypes, proembryos or embryogenic cells are formed by cleavage of the zygotic embryos and that the development of these adventitious embryos, normally hampered, can take place in vivo or in vitro as a result of two different mechanisms that prevent the dominance of the initial zygotic embryo. PMID:20675656

  18. Evaluating citrus germplasm for huanglongbing (HLB) resistance: USDA-ARS Inoculation Program

    Science.gov (United States)

    The Asian citrus psyllid (ACP), Diaphorina citri, is an important pest because it vectors bacteria responsible for a serious disease of citrus known as huanglongbing (citrus greening disease). USDA-ARS researchers recently established a program for screening citrus germplasm for resistance to the di...

  19. Consideration for alternative outlet for new citrus hybrids

    Science.gov (United States)

    Citrus sinensis (sweet orange, ex. Hamlin, Midsweet, Valencia) is the source of “orange juice” and juice must contain no less than 90% C. sinensis to be marketed as such. Juice produced from Citrus reticulata (mandarins) and C. reticulata hybrids (Orlando, Murcott, Fallglo, Sunburst, Minneola) can b...

  20. Sensory and quality evaluation of selected citrus hybrids

    Science.gov (United States)

    Increasingly consumers are becoming more sophisticated in their demand for diversity of products, greater health potential and good eating quality. The evaluation of a population of mandarin citrus (Citrus reticulata) and mandarin hybrids, was initiated in 2006-2007 with the goal of establishing bas...

  1. Citrus Tristeza Virus on the Island of Crete

    DEFF Research Database (Denmark)

    Shegani, M.; Tsikou, D.; Velimirovic, A.;

    2012-01-01

    Over a period of two years, more than 5,000 citrus trees were tested for the presence of Citrus tristeza virus (CTV) on the island of Crete, resulting in thirty eight positives. Comparisons of the relative transcript levels of CTV p23, coat protein (CP), polymerase (POL) and an intergenic (POL/p3...

  2. Microwave extraction of citrus peel to release pectin

    Science.gov (United States)

    After removal of soluble sugars and other compounds by washing, citrus peel is largely composed of pectin, cellulose and hemicellulose. In order to utilize the greatest amount of citrus peel product, it would appear reasonable that one or all three of these polysaccharides be converted to a useful m...

  3. Utilization of founder lines for improved Citrus biotechnology via RMCE

    Science.gov (United States)

    On October 1st 2011 the CRB chose to fund a unique research project, the development of citrus cultivars specifically for genetic engineering (GE). The objective of this research was to develop GE citrus ‘Founder Lines’ containing DNA sequences that will allow the precise insertion of genes for de...

  4. Discovery of a viral pathogen in the Asian citrus psyllid

    Science.gov (United States)

    We used a Metagenomics approach and discovered an insect-infecting virus in adult Asian citrus psyllids in Florida. Though wide spread in nature, this is the first report of a Fijivirus in North America. The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is a small insect tha...

  5. Developing cryotherapy to eliminate graft-transmissible pathogens in citrus

    Science.gov (United States)

    This article summarizes research being conducted as part of a project funded by the California Citrus Research Board to develop cryotherapy (freezing buds in liquid nitrogen, and then recovering them) as a viable method for elimination of graft transmissible pathogens from Citrus. There are current...

  6. Characterizing the citrus cultivar Carrizo genome through 454 shotgun sequencing.

    Science.gov (United States)

    Belknap, William R; Wang, Yi; Huo, Naxin; Wu, Jiajie; Rockhold, David R; Gu, Yong Q; Stover, Ed

    2011-12-01

    The citrus cultivar Carrizo is the single most important rootstock to the US citrus industry and has resistance or tolerance to a number of major citrus diseases, including citrus tristeza virus, foot rot, and Huanglongbing (HLB, citrus greening). A Carrizo genomic sequence database providing approximately 3.5×genome coverage (haploid genome size approximately 367 Mb) was populated through 454 GS FLX shotgun sequencing. Analysis of the repetitive DNA fraction indicated a total interspersed repeat fraction of 36.5%. Assembly and characterization of abundant citrus Ty3/gypsy elements revealed a novel type of element containing open reading frames encoding a viral RNA-silencing suppressor protein (RNA binding protein, rbp) and a plant cytokinin riboside 5′-monophosphate phosphoribohydrolase-related protein (LONELY GUY, log). Similar gypsy elements were identified in the Populus trichocarpa genome. Gene-coding region analysis indicated that 24.4% of the nonrepetitive reads contained genic regions. The depth of genome coverage was sufficient to allow accurate assembly of constituent genes, including a putative phloem-expressed gene. The development of the Carrizo database (http://citrus.pw.usda.gov/) will contribute to characterization of agronomically significant loci and provide a publicly available genomic resource to the citrus research community.

  7. 21 CFR 74.302 - Citrus Red No. 2.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Citrus Red No. 2. 74.302 Section 74.302 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES SUBJECT TO CERTIFICATION Foods § 74.302 Citrus Red No. 2. (a) Identity. (1) The color...

  8. Penicillium digitatum metabolites on synthetic media and citrus fruits

    DEFF Research Database (Denmark)

    Ariza, M.R.; Larsen, Thomas Ostenfeld; Petersen, Bent O.;

    2002-01-01

    Penicillium digitatum has been cultured on citrus fruits and yeast extract sucrose agar media (YES).Cultivation of fungal cultures on solid medium allowed the isolation of two novel tryptoquivaline-like metabolites, tryptoquialanine A (1) and tryptoquialanine B (2), also biosynthesized on citrus...

  9. Comparative study of LFY homologous genes among Citrus and its relative genre plants%柑橘及其近缘属植物LFY同源基因的比较

    Institute of Scientific and Technical Information of China (English)

    何新华; 郭永泽; 佘金彩; 李杨瑞

    2007-01-01

    LFY基因处于成花调控网络的关键位置,不仅调控开花时间和花转变,而且在花序和花的发育中也起重要作用.为了进一步探讨柑橘及其近缘属植物开花的分子机理,利用PCR技术分别从兴津温州蜜柑(Citrus unshiu Marcovitch)、无核椪柑(Citrus reticulata Blanco)、沙田柚[Citrus grandis(L.)Osbeck]融安金柑(Fortunella crassifolia Swing)和无核黄皮[Clausena lansium(Lour.)Skeels]叶片中分离克隆了LFY全长同源基因.结果表明兴津温州蜜柑、无核椪柑、沙田柚、融安金柑和无核黄皮中的LFY全长同源基因的核苷酸长度分别为2 090、2 086、2 092、2081、2 089bp,分别编码398、398、398、398和397个氨基酸,这些同源基因均由3个外显子和2个内含子组成.同源性分析发现,这些LFY全长同源基因的核苷酸序列和氨基酸序列同源性高,分别为92%~99%和95%~100%.亲缘关系分析结果与当前的植物学分类结果一致.

  10. Somatic Embryogenesis: Still a Relevant Technique in Citrus Improvement.

    Science.gov (United States)

    Omar, Ahmad A; Dutt, Manjul; Gmitter, Frederick G; Grosser, Jude W

    2016-01-01

    The genus Citrus contains numerous fresh and processed fruit cultivars that are economically important worldwide. New cultivars are needed to battle industry threatening diseases and to create new marketing opportunities. Citrus improvement by conventional methods alone has many limitations that can be overcome by applications of emerging biotechnologies, generally requiring cell to plant regeneration. Many citrus genotypes are amenable to somatic embryogenesis, which became a key regeneration pathway in many experimental approaches to cultivar improvement. This chapter provides a brief history of plant somatic embryogenesis with focus on citrus, followed by a discussion of proven applications in biotechnology-facilitated citrus improvement techniques, such as somatic hybridization, somatic cybridization, genetic transformation, and the exploitation of somaclonal variation. Finally, two important new protocols that feature plant regeneration via somatic embryogenesis are provided: protoplast transformation and Agrobacterium-mediated transformation of embryogenic cell suspension cultures.

  11. Dissipation and residue of forchlorfenuron in citrus fruits.

    Science.gov (United States)

    Chen, Weijun; Jiao, Bining; Su, Xuesu; Zhao, Qiyang; Qin, Dongmei; Wang, Chengqiu

    2013-06-01

    Field trials were carried out in three provinces of China to study the dissipation and residue of forchlorfenuron in citrus fruits. The results had shown that the degradation rate of forchlorfenuron in citrus fruits followed the first-order kinetics equation C = A∙eBt. The half-lives of forchlorfenuron were 15.8-23.0 days, the final residues of forchlorfenuron in pulp were all ≤0.002 mg/kg, and most of the residues were concentrated in the peel. The risk assessment revealed that no significant potential health risk would be induced by forchlorfenuron in citrus fruits. Therefore, it could be safe to apply forchlorfenuron in citrus fruits, and the results of this study could also be regarded as a reference to the setting of maximum residue limit for forchlorfenuron in citrus fruits in China.

  12. Investigation of heat treating conditions for enhancing the anti-inflammatory activity of citrus fruit (Citrus reticulata) peels.

    Science.gov (United States)

    Ho, Su-Chen; Lin, Chih-Cheng

    2008-09-10

    In traditional Chinese medicine, dried citrus fruit peels are widely used as remedies to alleviate coughs and reduce phlegm in the respiratory tract. Induction of inducible nitric oxide synthase (iNOS) in inflammatory cells and increased airway production of nitric oxide (NO) are well recognized as key events in inflammation-related respiratory tract diseases. Despite the fact that the enhancing effect of heat treatment on the antioxidant activity of citrus fruit peels has been well documented, the impact of heat treatment on citrus peel beneficial activities regarding anti-inflammation is unclear. To address this issue, we determined the anti-inflammatory activities of heat-treated citrus peel extracts by measuring their inhibitory effect upon NO production by lipopolysaccharide-activated RAW 264.7 macrophages. Results showed that the anti-inflammatory activity of citrus peel was significantly elevated after 100 degrees C heat treatment in a time-dependent fashion during a period from 0 to 120 min. Inhibition of iNOS gene expression was the major NO-suppressing mechanism of the citrus peel extract. Additionally, the anti-inflammatory activity of citrus peel extract highly correlated with the content of nobiletin and tangeretin. Conclusively, proper and reasonable heat treatment helped to release nobiletin and tangeretin, which were responsible for the increased anti-inflammatory activity of heat-treated citrus peels. PMID:18683945

  13. Metabolic Interplay between the Asian citrus psyllid and its Profftella symbiont: An Achilles’ heel of the citrus greening insect vector

    Science.gov (United States)

    ‘Candidatus Liberibacter asiaticus’ (CLas), the bacterial pathogen associated with citrus greening disease, is transmitted by Diaphorina citri, the Asian citrus psyllid. Interactions among D. citri and its microbial endosymbionts, including ‘Candidatus Profftella armatura’, are likely to impact tra...

  14. Seasonal Movement Patterns and Long-Range Dispersal of Asian Citrus Psyllid in Florida Citrus.

    Science.gov (United States)

    Lewis-Rosenblum, Hannah; Martini, Xavier; Tiwari, Siddharth; Stelinski, Lukasz L

    2015-02-01

    The Asian citrus psyllid, Diaphorina citri Kuwayama, is the vector of the bacterial pathogen, Candidatus Liberibacter asiaticus, which is the causal agent of huanglongbing (HLB) in the United States. Both short-range and long-range dispersal of D. citri adults affect the spread of HLB; however, little is known about the long-range dispersal capabilities of D. citri in the field or the seasonality of flight behavior. In the present study, an in situ protein marking technique was used to determine the dispersal of D. citri by trapping marked adults under natural field conditions. D. citri movement from abandoned citrus groves to adjacent managed citrus groves was greatest during the spring and summer months and decreased significantly during the colder months (September-March). D. citri were able to traverse potential geographic barriers such as roads and fallow fields. In an experiment conducted to determine long-range dispersal capacity in the absence of severe weather events, D. citri were able to disperse at least 2 km within 12 d. Wind direction was not correlated with the number of marked psyllids captured, indicating substantial flight capability by D. citri. Finally, the number of marked psyllids captured increased with the density of emerging young leaves on surrounding trees. The results confirm that abandoned citrus groves in Florida serve as reservoirs for D. citri, which can disperse across long distances despite geographical barriers. PMID:26470097

  15. Comparison of FTIR spectra between huanglongbing (citrus greening) and other citrus maladies

    Science.gov (United States)

    Fourier Transform Infrared Spectroscopy has the ability to quickly identify the presence of specific carbohydrates in plant materials. The presence of the disease Huanglongbing(HLB) in the leaves of infected citrus plants has a distinctive spectrum that can be used to distinguish an infected plant ...

  16. Seasonal Movement Patterns and Long-Range Dispersal of Asian Citrus Psyllid in Florida Citrus.

    Science.gov (United States)

    Lewis-Rosenblum, Hannah; Martini, Xavier; Tiwari, Siddharth; Stelinski, Lukasz L

    2015-02-01

    The Asian citrus psyllid, Diaphorina citri Kuwayama, is the vector of the bacterial pathogen, Candidatus Liberibacter asiaticus, which is the causal agent of huanglongbing (HLB) in the United States. Both short-range and long-range dispersal of D. citri adults affect the spread of HLB; however, little is known about the long-range dispersal capabilities of D. citri in the field or the seasonality of flight behavior. In the present study, an in situ protein marking technique was used to determine the dispersal of D. citri by trapping marked adults under natural field conditions. D. citri movement from abandoned citrus groves to adjacent managed citrus groves was greatest during the spring and summer months and decreased significantly during the colder months (September-March). D. citri were able to traverse potential geographic barriers such as roads and fallow fields. In an experiment conducted to determine long-range dispersal capacity in the absence of severe weather events, D. citri were able to disperse at least 2 km within 12 d. Wind direction was not correlated with the number of marked psyllids captured, indicating substantial flight capability by D. citri. Finally, the number of marked psyllids captured increased with the density of emerging young leaves on surrounding trees. The results confirm that abandoned citrus groves in Florida serve as reservoirs for D. citri, which can disperse across long distances despite geographical barriers.

  17. Detection of a new medium for budwood culture in vitro of citrus

    OpenAIRE

    SERTKAYA, Gülşen ŞAŞ; ÇINAR, Ahmet

    1998-01-01

    The transfer of new and quality citrus species and cultivars from one country or growing area to another may lead to the introduction of new pests and diseases. Therefore the import and direct production of citrus budwood without adequate control measures has a high risk. The citrus virus and virus-like disease agents can be successfully eliminated using the shoot-tip grafting (STG) technique which is routinely used to obtain virus-free plants in citrus improvement programs in major citrus...

  18. 76 FR 78228 - Notice of Request for Extension of Approval of an Information Collection; Citrus Greening and...

    Science.gov (United States)

    2011-12-16

    ... regulations to implement the PPA. Citrus greening, also known as Huanglongbing disease of citrus, is considered to be one of the most serious citrus diseases in the world. Citrus greening is a bacterial disease... the fruit, and can kill trees. Once infected, there is no cure for a tree with citrus greening...

  19. Chemical control of the Asian citrus psyllid and of huanglongbing disease in citrus.

    Science.gov (United States)

    Boina, Dhana Raj; Bloomquist, Jeffrey R

    2015-06-01

    By 2014, huanglongbing (HLB), the most destructive disease of citrus, and its insect vector, the Asian citrus psyllid (ACP), Diaphorina citri (Kuwayama), became established in all major citrus-growing regions of the world, including the United States, with the exception of California. At present, application of insecticides is the most widely followed option for reducing ACP populations, while application of antibiotics for suppressing HLB disease/symptoms is being practiced in some citrus-growing regions. Application of insecticides during the dormant winter season, along with cultivation of HLB-free seedlings and early detection and removal of symptomatic and asymptomatic trees, has been very effective in managing ACP. Area-wide management of ACP by application of insecticides at low volume in large areas of citrus cultivation has been shown to be effective in managing HLB and reducing management costs. As insecticide resistance is a major problem in sustainable management of ACP, rotation/alternation of insecticides with different chemistries and modes of action needs to be followed. Besides control of the insect vector, use of antibiotics has temporarily suppressed the symptoms of HLB in diseased trees. Recent efforts to discover and screen existing as well as new compounds for their antibiotic and antimicrobial activities have identified some promising molecules for HLB control. There is an urgent need to find a sustainable solution to the HLB menace through chemical control of ACP populations and within HLB-infected trees through the judicious use of labeled insecticides (existing and novel chemistries) and antibiotics in area-wide management programs with due consideration to the insecticide resistance problem.

  20. Chemical control of the Asian citrus psyllid and of huanglongbing disease in citrus.

    Science.gov (United States)

    Boina, Dhana Raj; Bloomquist, Jeffrey R

    2015-06-01

    By 2014, huanglongbing (HLB), the most destructive disease of citrus, and its insect vector, the Asian citrus psyllid (ACP), Diaphorina citri (Kuwayama), became established in all major citrus-growing regions of the world, including the United States, with the exception of California. At present, application of insecticides is the most widely followed option for reducing ACP populations, while application of antibiotics for suppressing HLB disease/symptoms is being practiced in some citrus-growing regions. Application of insecticides during the dormant winter season, along with cultivation of HLB-free seedlings and early detection and removal of symptomatic and asymptomatic trees, has been very effective in managing ACP. Area-wide management of ACP by application of insecticides at low volume in large areas of citrus cultivation has been shown to be effective in managing HLB and reducing management costs. As insecticide resistance is a major problem in sustainable management of ACP, rotation/alternation of insecticides with different chemistries and modes of action needs to be followed. Besides control of the insect vector, use of antibiotics has temporarily suppressed the symptoms of HLB in diseased trees. Recent efforts to discover and screen existing as well as new compounds for their antibiotic and antimicrobial activities have identified some promising molecules for HLB control. There is an urgent need to find a sustainable solution to the HLB menace through chemical control of ACP populations and within HLB-infected trees through the judicious use of labeled insecticides (existing and novel chemistries) and antibiotics in area-wide management programs with due consideration to the insecticide resistance problem. PMID:25491482

  1. MARCADORES RAPD PARA MAPEAMENTO GENÉTICO E SELEÇÃO DE HÍBRIDOS DE CITROS RAPD MARKERS TO GENETIC MAPPING AND SELECTION OF CITRUS HYBRIDS

    Directory of Open Access Journals (Sweden)

    ROBERTO PEDROSO DE OLIVEIRA

    2001-12-01

    Full Text Available Os marcadores moleculares apresentam várias aplicações no melhoramento de plantas, permitindo uma série de análises genéticas. Este trabalho foi realizado com o objetivo de estabelecer marcadores RAPD para serem utilizados em estudos de mapeamento genético e na seleção de híbridos entre tangerina-'Cravo' (Citrus reticulata Blanco e laranja-'Pêra' (C. sinensis (L. Osbeck. Extraiu-se DNA de folhas dos parentais e de seis híbridos F1. As reações de amplificação foram preparadas em 13 uL de solução, constituída por tampão 1x GIBCO BRL; soluções 1,54 mM de MgCl2 e 0,2 mM de cada dNTP; 15 ng de cada 'primer'; 1,5 unidade de 'Taq DNA Polymerase' e 15 ng de DNA genômico. As reações foram realizadas em termocicladores programados para 36 ciclos de 1 min a 92ºC, 1 min a 36ºC, 2 min a 72ºC e 10 min de extensão a 72ºC. Foram testados 'primers' decâmeros arbitrários dos 'kits' A, AB, AT, AV, B, C, D, E, G, H, M, N, P, Q, R e U da Operon, sendo selecionados 113 por apresentarem polimorfismo, com número de marcadores variando de 1 a 6 por 'primer'. Esses 'primers' amplificaram 201 (23,13% bandas polimórficas, aplicáveis no mapeamento genético e seleção de híbridos. A freqüência de 'primers' com 1; 2; 3; 4; 5 e 6 bandas polimórficas foi de 49,5%, 33,6%, 9,7%, 4,4%, 1,8% e 1,0%, respectivamente.Molecular markers have many applications in plant breeding, enabling some types of genetic analyses. The aim of this work was to establish RAPD markers to be used to genetic mapping studies and selection of hybrids between 'Cravo' tangerine (Citrus reticulata Blanco and 'Pêra' orange (C. sinensis (L. Osbeck. DNA of the parents and six hybrids F1 was isolated from the leaves. The amplification reactions were performed in volumes of 13 µL, composed by GIBCO BRL 1x buffer, 1,54 mM MgCl2, 0,2 mM of each dNTP, 15 ng of each primer, 1,5 unit of Taq DNA Polymerase and 15 ng of genomic DNA. These reactions were carried out in

  2. Comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches.

    Science.gov (United States)

    Ma, Qiaoli; Ding, Yuduan; Chang, Jiwei; Sun, Xiaohua; Zhang, Li; Wei, Qingjiang; Cheng, Yunjiang; Chen, Lingling; Xu, Juan; Deng, Xiuxin

    2014-01-01

    Auxin-like 2,4-dichlorophenoxyacetic acid (2,4-D), a high-efficiency anti-stalling agent for the post-harvest fresh fruit industry, has had its use restricted due to environmental concerns. However, no other substitutes for 2,4-D are available to the post-harvest industry. Insights into the molecular mechanism underlying the effects of 2,4-D on fruit quality preservation will provide a theoretical basis for exploring new safe and effective anti-stalling agents. This study comprehensively analysed changes in the peel of Olinda Valencia orange [Citrus sinensis (L.) Osbeck] induced by 500 ppm 2,4-D using 'omic'-driven approaches. Transcriptional profiling revealed that transcriptional factor (mainly AP2/ERF, WRKY, and NAC family members), transport, and hormone metabolism genes were over-represented and up-regulated within 24h post-treatment (HPT). Stress defence genes were up-regulated, while cell wall metabolism genes were down-regulated after 48 HPT. However, secondary metabolism genes, especially phenylpropanoid and lignin biosynthesis-related genes, were over-represented at all the time points. Comparative proteomic analysis indicated that the expression of proteins implicated in stress responses (25%), hormone metabolism, and signal transduction (12%) significantly accumulated at the post-transcriptional level. Hormone levels detected by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) showed that abscisic acid, salicylic acid, and 2,4-D significantly increased, while ethylene production (detected by gas chromatography) decreased after 2,4-D treatment. In addition, lignin and water content in the fruit peel also increased and the epicuticle wax ultrastructure was modified. In conclusion, 2,4-D retarded fruit senescence by altering the levels of many endogenous hormones and by improving stress defence capabilities by up-regulating defence-related genes and proteins.

  3. Comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches.

    Science.gov (United States)

    Ma, Qiaoli; Ding, Yuduan; Chang, Jiwei; Sun, Xiaohua; Zhang, Li; Wei, Qingjiang; Cheng, Yunjiang; Chen, Lingling; Xu, Juan; Deng, Xiuxin

    2014-01-01

    Auxin-like 2,4-dichlorophenoxyacetic acid (2,4-D), a high-efficiency anti-stalling agent for the post-harvest fresh fruit industry, has had its use restricted due to environmental concerns. However, no other substitutes for 2,4-D are available to the post-harvest industry. Insights into the molecular mechanism underlying the effects of 2,4-D on fruit quality preservation will provide a theoretical basis for exploring new safe and effective anti-stalling agents. This study comprehensively analysed changes in the peel of Olinda Valencia orange [Citrus sinensis (L.) Osbeck] induced by 500 ppm 2,4-D using 'omic'-driven approaches. Transcriptional profiling revealed that transcriptional factor (mainly AP2/ERF, WRKY, and NAC family members), transport, and hormone metabolism genes were over-represented and up-regulated within 24h post-treatment (HPT). Stress defence genes were up-regulated, while cell wall metabolism genes were down-regulated after 48 HPT. However, secondary metabolism genes, especially phenylpropanoid and lignin biosynthesis-related genes, were over-represented at all the time points. Comparative proteomic analysis indicated that the expression of proteins implicated in stress responses (25%), hormone metabolism, and signal transduction (12%) significantly accumulated at the post-transcriptional level. Hormone levels detected by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) showed that abscisic acid, salicylic acid, and 2,4-D significantly increased, while ethylene production (detected by gas chromatography) decreased after 2,4-D treatment. In addition, lignin and water content in the fruit peel also increased and the epicuticle wax ultrastructure was modified. In conclusion, 2,4-D retarded fruit senescence by altering the levels of many endogenous hormones and by improving stress defence capabilities by up-regulating defence-related genes and proteins. PMID:24215076

  4. Chemistry and Pharmacology of Citrus sinensis.

    Science.gov (United States)

    Favela-Hernández, Juan Manuel J; González-Santiago, Omar; Ramírez-Cabrera, Mónica A; Esquivel-Ferriño, Patricia C; Camacho-Corona, María del Rayo

    2016-01-01

    Presently the search for new drugs from natural resources is of growing interest to the pharmaceutical industry. Natural products have been the source of new drugs since ancient times. Plants are a good source of secondary metabolites which have been found to have beneficial properties. The present study is a review of the chemistry and pharmacology of Citrus sinensis. This review reveals the therapeutic potential of C. sinensis as a source of natural compounds with important activities that are beneficial for human health that could be used to develop new drugs. PMID:27072414

  5. Antibacterial activity of Citrus reticulata peel extracts.

    Science.gov (United States)

    Jayaprakasha, G K; Negi, P S; Sikder, S; Rao, L J; Sakariah, K K

    2000-01-01

    Citrus peels were successively extracted with hexane, chloroform and acetone using a soxhlet extractor. The hexane and chloroform extracts were fractionated into alcohol-soluble and alcohol-insoluble fractions. These fractions were tested against different gram positive and gram negative bacteria. The EtOH-soluble fraction was found to be most effective. Fractionation of EtOH-soluble fraction on silica gel column yielded three polymethoxylated flavones, namely desmethylnobiletin, nobiletin and tangeretin. Their structures were confirmed by UV, 1H, 13C NMR and mass spectral studies. The findings indicated a potential of these natural compounds as biopreservatives in food applications. PMID:11204182

  6. Citrus huanglongbing: a newly relevant disease presents unprecedented challenges.

    Science.gov (United States)

    Wang, Nian; Trivedi, Pankaj

    2013-07-01

    Citrus huanglongbing (HLB) is one of the oldest citrus diseases and has been known for over a century. HLB is caused by 'Candidatus Liberibacter' spp. that are phloem-limited, fastidious α-proteobacteria and infect hosts in different Kingdoms (i.e., Animalia and Plantae). When compared with well-characterized, cultivatable plant-pathogenic Gram-negative bacteria, the interactions of uncultured insect-vectored plant-pathogenic bacteria, including 'Ca. Liberibacter' spp., with their hosts remain poorly understood. 'Ca. Liberibacter' spp. have been known to cause HLB, which has been rapidly spreading worldwide, resulting in dramatic economic losses. HLB presents an unprecedented challenge to citrus production. In this review, we focus on the most recent research on citrus, 'Candidatus Liberibacter asiaticus', and psyllid interactions, specifically considering the following topics: evolutionary relationships among 'Ca. Liberibacter' spp., genetic diversity, host range, genome analysis, transmission, virulence mechanisms, and the ecological importance of HLB. Currently, no efficient management strategy is available to control HLB, although some promising progress has been made. Further studies are needed to understand citrus, 'Ca. L. asiaticus', and psyllid interactions to design innovative management strategies. Although HLB has been problematic for over a century, we can only win the battle against HLB with a coordinated and deliberate effort by the citrus industry, citrus growers, researchers, legislatures, and governments.

  7. Antimutagenicity and Anticancer Effects of Citrus Medica Fruit Juice

    Directory of Open Access Journals (Sweden)

    Majd Ahmad

    2009-10-01

    Full Text Available Currently cancer is considered as one of the main factors of mortality globally. Many chemicals in our environment can cause genetic mutations and are potentially responsible for millions of cancer-related deaths. Nowadays the scientists are looking for food materials which can potenthially prevent the cancer occurrence. The purpose of this research is to examine antimutagenicity and anticancer effect of Citrus Medica fruit juice.In present study human astrocytoma cancer cells were cultured in DMEM (Gibco,supplemented with 10% fetal calf serum,peniciline-streptomycin,L-glutamine and incubated at 37 ºC for 2 days.In addition cancer cell line were treated by half-ripe and ripe Citrus Medica fruit juice and cellular vital capacity was determined by MTT. The Citrus Medica fruit juice was subsequenthy evaluated in terms of antimutagenicity and anticancer properties by a standard reverse mutation assay (Ames Test. This was performed with histidine auxotroph strain of Salmonella typhimurium (TA100 .Thus, it requires histidine from a foreign supply to ensure its growth.The aforementioned strain gives rise to reverted colonies when expose to carcinogen substance (Sodium Azide. During MTT, human astrocytoma cell line revealed to have a meaningful cell death when compared with controls (P<0.01. In Ames Test the fruit juice prevented the reverted mutations and the hindrance percent of half-ripe Citrus Medica was 71.7% and ripe Citrus Medica was 34.4% in antimutagenicity test and this value in anticancer test was 83.3% and 50% in half-ripe Citrus Medica and ripe Citrus Medica respectively.This is the first study that have revealed antimutagenicity and anticancer effect of Citrus Medica fruit juice and the effects were higher in half-ripe Citrus Medica in comparison to the riprned one.

  8. Relações entre a produção de laranjeira 'Westin' e as precipitações em Botucatu, SP Relationships between production of 'Westin' sweet orange trees and rainfall at Botucatu, São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    Antonio Tubelis

    1999-05-01

    Full Text Available O trabalho estuda a correlação entre a produção de um pomar de laranja, plantado no altiplano de Botucatu, SP, com as precipitações que ocorrem dezesseis meses antes da colheita e a idade do pomar. As plantas eram de laranjeira doce (Citrus sinensis (L. Osbeck, variedade Westin, de clone nucelar, enxertadas em porta-enxerto de limoeiro 'Cravo' (Citrus limonia Osbeck, plantadas em solo Terra Roxa Estruturada, a 810 m de altitude e em região de clima do tipo Cwb. A cultura foi conduzida de modo convencional e sem irrigação. Coletaram-se dados de produção, nos períodos entre o 3º e o 17º e entre o 21º e o 27º ano de idade do pomar, para análise do comportamento da produção e o efeito da idade e das precipitações na produção. Calcularam-se equações lineares múltiplas de regressão, entre a produção, idade do pomar e as precipitações mensais, nos períodos de pomar juvenil, adulto, senescente e adulto-senescente. A produção correlacionou-se com a idade e com valores mensais de precipitação. Os pequenos desvios observados entre os valores medidos e estimados de produção revelaram que as equações de regressão poderiam ser usadas na previsão de safra ou no controle de irrigação suplementar do pomar.This paper deals with the existence of correlation between the production of a sweet orange orchard, planted at the plateau of Botucatu, São Paulo State, Brazil, with the orchard age and the rainfall that occurred in the sixteen months before the picking season. The plants were of sweet orange trees (Citrus sinensis (L. Osbeck, variety Westin, budded on 'Rangpur' lime (Citrus limonia Osbeck rootstock, grown on "Terra Roxa Estruturada" soil, at an altitude of 810 m above sea level and in a region with Cwb climatic type. The orchard was conducted by conventional ways and no irrigation was applied. The production of the orchard was recorded, during the period from the 3rd until the 17th and from the 21st until the 27th

  9. INFLUENCE OF CITRUS LEPROSIS ON THE MINERAL COMPOSITION OF Citrus sinensis LEAVES

    Directory of Open Access Journals (Sweden)

    N. de L. Nogueira

    1996-05-01

    Full Text Available Citrus leprosis is the main viral disease affecting citrus in Brazil. Viral diseases can affect the mineral composition of citrus leaves, inducing mineral deficiencies. The aim of this work was to verify the influence of citrus leprosis on the mineral composition of citrus leaves, cultivar Pera. Orange seedlings were grown in pots containing sterile soil, and kept under greenhouse conditions. When plants had 15 to 20 leaves, they were infested with the mite Brevipalpus phoenicis, the leprosis vector, collected from infected citrus plants. Other plants were not infested, and were used as the control. Ninety days after infestation, all the infested plants presented leprosis lesions. The six upper leaves of each plant were collected and the determination of macro and micro-elements was performed. Leaves from the infected plants showed lower levels of N and higher levels of Ca, S and Fe, as compared to the control (= 0.05. Both treatments presented similar levels of P, K, Mg, Cu,Mn, Zn and B.A leprose dos citros é uma das principais doenças de citros no Brasil. As doenças causadas por vírus podem afetar a composição mineral de folhas de citros, induzindo deficiências minerais. Este trabalho teve como objetivo analisar a influência da leprose dos citros na composição mineral de folhas de citros, cultivar Pera. Seedlings de laranja foram crescidos em vasos contendo solo estéril e mantidos em casa de vegetação. Plantas com 15 a 20 folhas foram infestadas com ácaro da espécie Brevipalpus phoenicis, vetor da leprose dos citros, provenientes de plantas de citros com sintomas da doença. Outras plantas não foram infestadas, e serviram como controle. Após noventa dias, todas as plantas infestadas apresentavam lesões de leprose. Seis folhas da parte superior de cada planta foram coletadas para determinação dos teores de macro e micronutrientes. As folhas das plantas infectadas apresentaram níveis mais baixos de N e mais elevados de Ca, S

  10. The Asian Citrus Psyllid Genome (Diaphorina citri, Hemiptera)

    OpenAIRE

    Hunter, Wayne B.; Reese, Justin; International Psyllid Genome Consortium, The

    2014-01-01

    The Asian citrus psyllid, Diaphorina citri (Hemiptera), is an important pest of citrus because it vectors bacteria responsible for huanglongbing, which is one of the most serious diseases of citrus worldwide.  The first genome draft of D. citri (DIACI_1.0) was completed in 2011 (ARS, Ft. Pierce, FL), however, gaps in the assembly prompted additional sequencing using the long run PacBio system at the Los Alamos National Lab, NM.  The revised draft genome (DIACI_1.1) was assembled using the new...

  11. Genome-wide comparative analysis reveals possible common ancestors of nucleotide-binding sites domain containing genes in hybrid Citrus sinensis genome and original Citrus clementina genome

    Science.gov (United States)

    We identified and re-annotated candidate disease resistance (R) genes with nucleotide-binding sites (NBS) domain from a Citrus clementina genome and two complete Citrus sinensis genome sequences (one from the USA and one from China). We found similar numbers of NBS genes from three citrus genomes, r...

  12. Phenology of Asian citrus psyllid (Hemiptera: Liviidae) and associated parasitoids on two species of Citrus, kinnow mandarin and sweet orange, in Punjab Pakistan.

    Science.gov (United States)

    Khan, Shouket Zaman; Arif, Muhammad Jalal; Hoddle, Christina D; Hoddle, Mark S

    2014-10-01

    The population phenology of Asian citrus psyllid, Diaphorina citri Kuwayama, was monitored weekly for 110 wk on two species of Citrus, kinnow mandarin and sweet orange, at two different research sites in Faisalabad, Punjab Pakistan. Citrus flush growth patterns were monitored and natural enemy surveys were conducted weekly. Flush patterns were similar for kinnow and sweet orange. However, flush on sweet orange was consistently more heavily infested with Asian citrus psyllid than kinnow flush; densities of Asian citrus psyllid eggs, nymphs, and adults were higher on sweet orange when compared with kinnow. When measured in terms of mean cumulative insect or Asian citrus psyllid days, eggs, nymphs, and adults were significantly higher on sweet orange than kinnow. Two parasitoids were recorded attacking Asian citrus psyllid nymphs, Tamarixia radiata (Waterston) and Diaphorencyrtus aligarhensis (Shafee, Alam and Agarwal). The dominant parasitoid species attacking Asian citrus psyllid nymphs on kinnow and sweet orange was T. radiata, with parasitism averaging 26%. D. aligarhensis parasitism averaged 17%. Generalist predators such as coccinellids and chrysopids were collected infrequently and were likely not important natural enemies at these study sites. Immature spiders, in particular, salticids and yellow sac spiders, were common and may be important predators of all Asian citrus psyllid life stages. Low year round Asian citrus psyllid densities on kinnow and possibly high summer temperatures, may, in part, contribute to the success of this cultivar in Punjab where Candidatus Liberibacter asiaticus, the putative causative agent of huanglongbing, a debilitating citrus disease, is widespread and vectored by Asian citrus psyllid.

  13. Phenology of Asian citrus psyllid (Hemiptera: Liviidae) and associated parasitoids on two species of Citrus, kinnow mandarin and sweet orange, in Punjab Pakistan.

    Science.gov (United States)

    Khan, Shouket Zaman; Arif, Muhammad Jalal; Hoddle, Christina D; Hoddle, Mark S

    2014-10-01

    The population phenology of Asian citrus psyllid, Diaphorina citri Kuwayama, was monitored weekly for 110 wk on two species of Citrus, kinnow mandarin and sweet orange, at two different research sites in Faisalabad, Punjab Pakistan. Citrus flush growth patterns were monitored and natural enemy surveys were conducted weekly. Flush patterns were similar for kinnow and sweet orange. However, flush on sweet orange was consistently more heavily infested with Asian citrus psyllid than kinnow flush; densities of Asian citrus psyllid eggs, nymphs, and adults were higher on sweet orange when compared with kinnow. When measured in terms of mean cumulative insect or Asian citrus psyllid days, eggs, nymphs, and adults were significantly higher on sweet orange than kinnow. Two parasitoids were recorded attacking Asian citrus psyllid nymphs, Tamarixia radiata (Waterston) and Diaphorencyrtus aligarhensis (Shafee, Alam and Agarwal). The dominant parasitoid species attacking Asian citrus psyllid nymphs on kinnow and sweet orange was T. radiata, with parasitism averaging 26%. D. aligarhensis parasitism averaged 17%. Generalist predators such as coccinellids and chrysopids were collected infrequently and were likely not important natural enemies at these study sites. Immature spiders, in particular, salticids and yellow sac spiders, were common and may be important predators of all Asian citrus psyllid life stages. Low year round Asian citrus psyllid densities on kinnow and possibly high summer temperatures, may, in part, contribute to the success of this cultivar in Punjab where Candidatus Liberibacter asiaticus, the putative causative agent of huanglongbing, a debilitating citrus disease, is widespread and vectored by Asian citrus psyllid. PMID:25198345

  14. Saponin-rich fraction from Clematis chinensis Osbeck roots protects rabbit chondrocytes against nitric oxide-induced apoptosis via preventing mitochondria impairment and caspase-3 activation.

    Science.gov (United States)

    Wu, Wenjun; Gao, Xinghua; Xu, Xianxiang; Luo, Yubin; Liu, Mei; Xia, Yufeng; Dai, Yue

    2013-03-01

    Our previous study reported that the saponin-rich fraction from Clematis chinensis Osbeck roots (SFC) could effectively alleviate experimental osteoarthritis induced by monosodium iodoacetate in rats through protecting articular cartilage and inhibiting local inflammation. The present study was performed to investigate the preventive effects of SFC on articular chondrocyte, and explore the underlying mechanisms. Primary rabbit chondrocytes were cultured and exposed to sodium nitroprusside (SNP), a NO donor. After treatment with different concentrations of SFC (30, 100, 300, 1,000 μg/ml) for 24 h, nucleic morphology, apoptotic rate, mitochondrial function and caspase-3 activity of chondrocytes were examined. The results showed that SNP induced remarkable apoptosis of rabbit chondrocytes evidenced by Hoechst 33258 staining and flow cytometry analysis, and SFC prevented the apoptosis in a concentration-dependent manner. Further studies indicated that SFC could prevent the depolarization of mitochondrial membrane potential (∆ψm) in SNP-treated chondrocytes and suppress the activation of caspase-3. It can be concluded that the protection of SFC on articular chondrocytes is associated with the anti-apoptosis effects via inhibiting the mitochondrion impairment and caspase-3 activation. PMID:22821055

  15. Therapeutic effect of the saponin fraction from Clematis chinensis Osbeck roots on osteoarthritis induced by monosodium iodoacetate through protecting articular cartilage.

    Science.gov (United States)

    Wu, Wenjun; Xu, Xianxiang; Dai, Yue; Xia, Lunzhu

    2010-04-01

    The objective of the present study was to investigate the effect of the saponin fraction from Clematis chinensis Osbeck roots (SFC) on an osteoarthritis model in rats and to explore its underlying mechanisms. Osteoarthritis was induced by intraarticular injection of monosodium iodoacetate (MIA) into knee joints of rats, and SFC and diclofenac were orally administered once a day for 28 consecutive days. Joint swelling, macroscopic observation, histological assessment and proteoglycan (PG) degradation were examined. In vitro, cultured rabbit chondrocytes were stimulated with MIA and sodium nitroprusside (SNP), respectively. The effects of SFC on MIA- and SNP-induced chondrocyte injury were examined by MTT assay. It was shown that SFC (50, 100, 200 mg/kg) dose-dependently reduced cartilage injury and PG degradation induced by MIA. Diclofenac (4 mg/kg) only slightly alleviated cartilage injury and PG degradation. SFC also prevented SNP- or MIA-induced rabbit chondrocyte impairment. These results indicate that SFC is effective in ameliorating joint destruction and cartilage erosion in MIA-induced osteoarthritic in rats, and the mechanisms of action for protecting articular cartilage are through preventing extracellular matrix degradation and chondrocyte injury. PMID:19655297

  16. Rentabilidade da tangerineira 'Ponkan' submetida ao raleio químico comparada com ao manejo convencional

    Directory of Open Access Journals (Sweden)

    Maria do Céu Monteiro Cruz

    2011-10-01

    Full Text Available A pesquisa foi conduzida em um pomar comercial, localizado no município de Perdões, região sul de Minas Gerais, com o objetivo de analisar e comparar a rentabilidade da produção da tangerineira 'Ponkan' (Citrus reticulata Blanco submetida ao raleio químico com o manejo convencional. Para se montar a matriz de coeficientes técnicos dos custos de produção e os indicadores de rentabilidade da cultura, os dados foram obtidos com os produtores da região e baseados em trabalho de pesquisa realizado no pomar, no período de dezembro de 2006 a julho de 2008. Foram utilizadas plantas com dez anos de enxertadas sobre limoeiro 'Cravo' (Citrus limonia Osbeck. No primeiro ano, o raleio químico foi realizado com aplicação de Ethephon na concentração de 600 mg L-1,e por ocasião da colheita foi avaliado o rendimento da produção nas plantas submetidas ao raleio químico e ao manejo convencional. No segundo ano, foi determinada a alternância de produção e a produtividade. A prática do raleio químico proporcionou maior rentabilidade, em termos de produtividade da tangerina 'Ponkan', que o manejo convencional, sem a adoção do raleio.

  17. Root distribution of rootstocks for 'Tahiti' lime

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    Neves Carmen Silvia Vieira Janeiro

    2004-01-01

    Full Text Available Field studies on citrus roots are important for genetic selection of cultivars and for management practices such as localized irrigation and fertilization. To characterize root systems of six rootstocks, taking into consideration chemical and physical characteristics of a clayey Typic Hapludox of the Northern State of Paraná, this study was performed having as scion the 'IAC-5 Tahiti' lime [Citrus latifolia (Yu. Tanaka]. The rootstocks 'Rangpur' lime (C. limonia Osbeck, 'Africa Rough' lemon (C. jambhiri Lush., 'Sunki' mandarin [C. sunki (Hayata hort. ex Tan.], Poncirus trifoliata (L. Raf., 'C13' citrange [C. sinensis (L. Osb. x P. trifoliata (L. Raf] and 'Catânia 2' Volkamer lemon (C. volkameriana Ten. & Pasq. were used applying the trench profile method and the SIARCS® 3.0 software to determine root distribution. 'C-13' citrange had the largest root system. 'Volkamer' lemon and 'Africa Rough' lemon presented the smallest amount of roots. The effective depth for 80 % of roots was 31-53 cm in rows and 67-68 cm in inter-rows. The effective distance of 80 % of roots measured from the tree trunk exceeded the tree canopy for P. trifoliata, 'Sunki' mandarin, and 'Volkamer' and 'Africa Rough' lemons.

  18. 锦橙皮膳食纤维抗氧化活性研究%Study on antioxidant activity of dietary fiber from Citrus sinensis Osbeck cv.Jincheng peel

    Institute of Scientific and Technical Information of China (English)

    李燮昕; 蒲彪

    2010-01-01

    目的:提取锦橙皮膳食纤维,研究其对O_2~-·、·OH、DPPH·三种自由基的清除作用.方法:采用复合酶法对膳食纤维进行提取,采用邻苯三酚自氧化法和Fronton反应对清除O_2~-·、·OH、DPPH·的效果进行了研究.结果:对O_2~-·清除率最高为46.28%,对·OH的IC_(50)为3.27mg/mL,对DPPH·的IC_(50)为5.66mg/mL.综上所述,锦橙皮膳食纤维时三种自由基都具有一定的清除作用.

  19. Identificación de aminoácidos libres por cromatografía de capa fina en jugo fresco de naranja (Citrus sinensis L. Osbeck variedad “Valencia”

    Directory of Open Access Journals (Sweden)

    Myrna Luisa Medina Bracamonte

    2013-12-01

    Full Text Available Con el interés de aportar al conocimiento de los aminoácidos libres en el jugo de naranja “Valencia” producido en Venezuela, se propuso aplicar cromatografía de capa fina, al jugo recién extraído de 2 lotes de naranjas “Valencia” adquiridas en mercados locales diferentes de la ciudad de Caracas. El jugo se centrifugó a 960 g (15 min-1. El sobrenadante se homogeneizó con igual volumen de etanol 95 % (v/v, por 3 s y se centrifugó a 900 g (15 min-1. Se ajustó el pH del sobrenadante a 1,7. Se pasó 30 mL del sobrenadante acondicionado a una columna de intercambio iónico de poliestireno activada en forma de H+ (6 x 1,7 cm. El volumen del eluato recogido se evaporó a 40 ºC a vacío hasta sequedad. El residuo seco se suspendió en 2,5 mL de una solución metanol:agua 50:50 (v/v a pH 1,7 y de allí se tomó una muestra de 5 μL con una micropipeta digital Calibra® 822, capacidad 2-20 μL y se aplicó sobre cromatofolios de sílica gel 60 para la cromatografía bidireccional: solvente I, cloroformo:metanol:amoníaco 25 % (v/v 40:40:20; solvente II, fenol:agua 80:20 (m/v. Hubo diferencias en el número de aminoácidos revelados e identificados entre los jugos de ambos lotes. Ambos cromatogramas coincidieron en 8 de los aminoácidos revelados: ácido aspártico, serina, alanina, valina, metionina, prolina, probablemente triptófano y/o fenilalanina y uno no identificado. En ambos predominó prolina y en ambos se identificó el ácido aspártico predominando en el lote 2 en proporción muy similar a la de prolina. El jugo del lote 2 se caracterizó por mayor índice de madurez y de nitrógeno aminoacídico que el jugo del lote 1, en donde el ácido aspártico estuvo en muy baja proporción. También se identificó metionina. Solo en el lote 1 se identificó lisina, ácido glutámico, asparagina y tirosina.

  20. Identificación de aminoácidos libres por cromatografía de capa fina en jugo fresco de naranja (Citrus sinensis L. Osbeck variedad "Valencia"

    Directory of Open Access Journals (Sweden)

    Myrna Luisa Medina Bracamonte

    2013-12-01

    Full Text Available Con el interés de aportar al conocimiento de los aminoácidos libres en el jugo de naranja "Valencia" producido en Venezuela, se propuso aplicar cromatografía de capa fina, al jugo recién extraído de 2 lotes de naranjas "Valencia" adquiridas en mercados locales diferentes de la ciudad de Caracas. El jugo se centrifugó a 960 g (15 min-1. El sobrenadante se homogeneizó con igual volumen de etanol 95 % (v/v, por 3 s y se centrifugó a 900 g (15 min-1. Se ajustó el pH del sobrenadante a 1,7. Se pasó 30 mL del sobrenadante acondicionado a una columna de intercambio iónico de poliestireno activada en forma de H+ (6 x 1,7 cm. El volumen del eluato recogido se evaporó a 40 ºC a vacío hasta sequedad. El residuo seco se suspendió en 2,5 mL de una solución metanol:agua 50:50 (v/v a pH 1,7 y de allí se tomó una muestra de 5 μL con una micropipeta digital Calibra® 822, capacidad 2-20 μL y se aplicó sobre cromatofolios de sílica gel 60 para la cromatografía bidireccional: solvente I, cloroformo:metanol:amoníaco 25 % (v/v 40:40:20; solvente II, fenol:agua 80:20 (m/v. Hubo diferencias en el número de aminoácidos revelados e identificados entre los jugos de ambos lotes. Ambos cromatogramas coincidieron en 8 de los aminoácidos revelados: ácido aspártico, serina, alanina, valina, metionina, prolina, probablemente triptófano y/o fenilalanina y uno no identificado. En ambos predominó prolina y en ambos se identificó el ácido aspártico predominando en el lote 2 en proporción muy similar a la de prolina. El jugo del lote 2 se caracterizó por mayor índice de madurez y de nitrógeno aminoacídico que el jugo del lote 1, en donde el ácido aspártico estuvo en muy baja proporción. También se identificó metionina. Solo en el lote 1 se identificó lisina, ácido glutámico, asparagina y tirosina.

  1. 杀菌方式对无核雪柑汁香气成分的影响%Effects of Sterilization Methods on Aromatic Components of Orange Juice from Seedless ‘Xuegan’ Sweet Orange (Citrus sinensis L. Osbeck)

    Institute of Scientific and Technical Information of China (English)

    郭莉; 黄明发; 吴厚玖; 王华; 沈海亮; 何朝飞

    2012-01-01

    采用固相微萃取-气相色谱-质谱法分析橙汁香气成分,内标法半定量,研究了巴氏杀菌和微波杀菌对无核雪柑汁香气成分的影响。结果表明:无核雪柑鲜榨汁、巴氏杀菌汁、微波杀菌汁分别检测出56种、57种和53种香气成分,含量分别为1304.70、991.51mg/kg和887.67mg/kg,巴氏杀菌后橙汁香气成分总含量高于微波杀菌汁,但微波杀菌后叶醇、十二碳醛、γ-芹子烯等含量均高于巴氏杀菌处理,且未检出异味物质β-松油醇,2种杀菌处理后烃类、酯类物质总含量均下降,酮类物质上升。%Solid-phase micro-extraction-gas chromatography-mass spectrometry(SPME-GC-MS) and internal standard method for semi-quantification were used to study the effects of pasteurization and microwave sterilization on aromatic components of seedless ‘Xuegan’ sweet orange juice.The results showed 56,57 and 53 aromatic compounds were respectively identified from native sweet orange juice,pasteurized sweet orange juice and microwave sterilized sweet orange juice,with respective contents of 1.30,0.99 mg/g and 0.89 mg/g.Although pasteurized sweet orange juice was richer in total aromatic compounds than microwave sterilized sweet orange,microwave sterilized sweet orange contained more cis-3-hexenol,dodecanal and γ-selinene than pasteurized sweet orange juice but no β-terpineol,responsible for off-odor.Pasteurization and microwave sterilization resulted in a decrease in the total content of esters and hydrocarbons and an increase in ketones.

  2. Isolation,Identification and cDNA Cloning of ACTIN of the Citrus grandis (L.) Osbeck Juice Sacs%琯溪蜜柚汁胞ACTIN的分离鉴定和cDNA克隆

    Institute of Scientific and Technical Information of China (English)

    钟凤林; 郭志雄; 潘东明

    2010-01-01

    对琯溪蜜柚粒化与正常汁胞汁胞双向电泳分析,获得一个在粒化中上调表达的羞异蛋白质点,经过质谱鉴定、生物信息学分析,为肌动蛋白(Actin),以琯溪蜜柚粒化汁胞cDNA为模板,根据Actin氨基酸序列设计引物,进行全长的扩增,得到Actin开放阅读框包含1134个碱基,编码377个氨基酸.其核酸序列与黑杨派(Populus trichocarpa)、棉花(Gossypium hirsutum)、蓖麻(Ricinus communis)、圆叶锦葵(Malva pusilla)ACT的同源性在89%以上.

  3. 琯溪蜜柚果肉过氧化物酶cDNA的克隆及序列分析%Cloning and Sequence analysis of the Citrus grandis (L.) Osbeck Flesh Peroxidase cDNA

    Institute of Scientific and Technical Information of China (English)

    林琳; 郭志雄; 潘东明

    2006-01-01

    以琯溪蜜柚果肉为材料,运用cDNA克隆的方法,克隆得到了琯溪蜜柚果肉过氧化物酶的cDNA的全长,并对得到的序列进行了一些分析,从分子生物学角度对粒化的研究做了初步的探索.结果表明:琯溪蜜柚果肉POD cDNA全长1263bp,有完整的阅读框,编码351个氨基酸.另外5'非翻译区42bp,3'非翻译区168bp(不包含终止密码子TAA),其中包括一个多聚腺苷酸化信号AATAAA,以及一个含24个腺苷酸的poly(A)尾.由琯溪蜜柚果肉POD cDNA推导的氨基酸序列与无花果(Ficus carica)、陆地棉(Gossypium hirsutum)、杨属植物(Populus)等同源性较高,分别为:58.6%,67.61%,65.24%,67.14%等.

  4. Monitoramento populacional das cigarrinhas vetoras de Xylella fastidiosa, através de armadilhas adesivas amarelas em pomares comerciais de citros Population monitoring of leafhopper vectors of Xylella fastidiosa, through yellow sticky traps in commercial citrus orchards

    Directory of Open Access Journals (Sweden)

    Rúbia de Oliveira Molina

    2010-12-01

    Full Text Available A Clorose variegada dos citros (CVC é uma doença causada pela bactéria de xilema Xylella fastidiosa Wells. A disseminação ocorre por meio de insetos vetores pertencente à ordem Hemiptera, família Cicadellidae (subfamília Cicadellinae, os quais transmitem a bactéria depois de se alimentarem em plantas contaminadas. Neste trabalho, objetivou-se identificar e monitorar as espécies de cigarrinhas vetoras em um pomar comercial no município de Paranavaí, Paraná. O experimento foi realizado em um talhão comercial de laranja doce (Citrus sinensis (L. Osbeck, variedade Pera, com 1.000 plantas de dez anos de idade. A amostragem foi realizada por meio de armadilhas adesivas amarelas, distribuídas na área periférica e central do pomar, com duas repetições por rua amostrada. As etiquetas foram distribuídas entre a 5ª e 30ª plantas em 10 ruas, totalizando 20 armadilhas que foram renovadas no pomar, a cada trinta dias, durante o período de avaliação que foi entre junho de 2005 e setembro de 2006. As principais espécies de Cicadellinae capturadas foram Acrogonia citrina Marucci & Cavichioli, Dilobopterus costalimai Young e Macugonalia cavifrons Stal. Essas espécies apresentaram ocorrência constante e frequência de 3,97%, 4,2%, 13,0% respectivamente, em relação ao total de cigarrinhas coletadas.Citrus variegated chlorosis (CVC is the disease caused by the bacteria Xylella fastidiosa Wells. Dissemination occurs through insect vectors belonging to the order Hemiptera, family Cicadellidae (subfamily Cicadellinae, which transmit the bacteria after feeding on infected plants. The objective of this study was to identify the species of insect vectors in an orchard in the municipality of Paranavaí, in the State of Paraná. The experiment was conducted in a commercial stand of sweet orange (Citrus sinensis (L. Osbeck, Pêra variety with 1,000 10-year-old plants. Monitoring was performed using yellow sticky traps, distributed in the central

  5. Spatial-temporal distribution of sharpshooters (Hemyptera: Cicadellidae insect vectors of Xylella fastidiosa in citrus orchards = Distribuição espaço-temporal de cigarrinhas (Hemiptera: Cicadellidae vetores da Xylella fastidiosa em pomares cítricos

    Directory of Open Access Journals (Sweden)

    Rúbia de Oliveira Molina

    2016-07-01

    Full Text Available Variegated chlorosis (CVC is a citrus disease, reported initially in the northwest of São Paulo state and in the Triângulo Mineiro region of Minas Gerais state in 1987. The CVC is caused by the xylematic bacteria Xylella fastidiosa. The bacteria is spread through contaminated bubbles or by insect vectors belonging to the Hemyptera order and Cicadellidae family. The aimed of this study was to identify the species of Xylella fastidiosa insect vector and to determine its spatial and temporal distribution in commercial orchards of sweet orange [Citrus sinensis (L. Osbeck]. The experiment was conducted in a commercial area of sweet orange, Pêra variety, grafted on Rangpur lime, located in northwest Paraná. For sampling, yellow sticky traps were used, distributed in the peripheral and central area of the orchard with four replicates per street sampled (5, 30, 55 and 80th plant, each plant was considered a sample unit. Were evaluated ten plots per street, totaling 40 traps for sampling. Every thirty days during the evaluation period, the traps were renewed in the orchard. The main species caught were Acrogonia citrine and Dilobopterus costalimai. The highest incidences occurred from winter to spring, and summer to autumn of the next year. According to the geostatistical analysis, the spatial distribution of these species concentrated in the peripheral zone of the portion where a higher incidence of these species was captured. The results show that it is necessary to adopt pest management practices for the Cicadellidae vector of X. fastidiosa differentiated in space and time. = A clorose variegada dos citros (CVC é uma doença de plantas cítricas, constatada, em 1987, inicialmente nos municípios do noroeste paulista e da região do triângulo mineiro. Ela é causada por uma bactéria de xilema, denominada Xylella fastidiosa. Sua disseminação ocorre através de borbulhas contaminadas ou por meio de insetos vetores da ordem Hemiptera e fam

  6. The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms

    Directory of Open Access Journals (Sweden)

    Sarah Almeida Coelho Oliveira

    2014-01-01

    Full Text Available The aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs compared to 0.2% chlorhexidine (CHX and 1% sodium hypochlorite (NaOCl on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10: C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05. C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7% was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases.

  7. Anti-inflammatory effects of Citrus sinensis L., Citrus paradisi L. and their combinations.

    Science.gov (United States)

    Khan, Rafeeq Alam; Mallick, Neelam; Feroz, Zeeshan

    2016-05-01

    Citrus bioflavonoids embrace a wide group of phenolic compounds effecting the production and scavenging of reactive oxygen species and the processes relating free radical-mediated injury. Keeping in view of the antioxidant and anti-inflammatory properties of Citrus sinensis and Citrus paradisi, present study was undertaken to explore the effects of C. sinensis (orange juice) and C. paradisi (grapefruit juice) at three different doses alone and their two combinations with the objective to examine the effects of these compounds in an experimental model of rat colitis induced by trinitrobenzenesulphonic acid (TNBS). Hence biochemical parameters e.g. myeloperoxidase, alkaline phosphatase, C-reactive protein (CRP) and glutathione were assessed. Data entry and analysis was accomplished by Statistical Package for the Social Sciences version 17 and was presented as mean ± S.E.M with 95% confidence interval. Present result shows that these juices, mainly C. paradisi, may be efficacious for the management of inflammatory bowel disease. In acute colitis model, C. paradise encouraged a decrease in the extension of the lesion escorted by a decrease in the occurrence of diarrhea and reinstatement of the glutathione content. Related effects were produced by the administration of C. sinensis, which also prevented the myeloperoxidase and alkaline phosphatase actions in acute intestinal inflammatory process. The effect of the citrus juices on the inflammatory process may be associated to their antioxidant and anti-inflammatory properties, as revealed in present investigation. The favorable effects exerted were demonstrated both by histological and biochemical changes and were related with a progress in the colonic oxidative status.

  8. Avaliação genética de seleções e híbridos de limões cravo, volkameriano e rugoso como porta-enxertos para laranjeiras Valência na presença da morte súbita dos citros Genetic evaluation of selections and hybrids of rangpur lime, volkamer and rough lemons rootstocks for Valência orange trees in the presence of the citrus sudden death

    Directory of Open Access Journals (Sweden)

    Jorgino Pompeu Junior

    2013-03-01

    Full Text Available Este estudo teve como objetivo realizar a avaliação genética da produção de frutos, eficiência produtiva e altura de laranjeiras Valência (Citrus sinensis enxertadas em seleções e híbridos dos limões Cravo (C. limonia, Volkameriano (C. volkameriana e Rugoso (C. jambhiri, em área endêmica para morte súbita dos citros (MSC. Foram avaliados 36 genótipos desses porta-enxertos, representados por cinco plantas cada, avaliados em cinco safras, do terceiro ao sétimo ano após o plantio. Sete dos genótipos avaliados apresentaram plantas com sintomas de MSC até o sétimo ano: Rangpur Otaheite orange 12901 (859, Rangpur Red Lime D.33.30 (866, Limão-Cravo EEL (871, Rangpur Borneo red (874, Citrus kokhai (1649, Limão-Rugoso 58329 (1655 e Limão- Cravo x Swingle B (1695. Para os genótipos que não manifestaram sintomas da doença, foram estimados parâmetros genéticos e fenotípicos e realizada a predição de valores genéticos dos indivíduos, visando à seleção e ao melhoramento genético para as características citadas, empregando-se o método REML/BLUP (máxima verossimilhança restrita/melhor predição linear não viciada. A análise de produção de frutos de cinco safras mostrou acurácia seletiva de 84,59%, tornando-se desnecessária a avaliação de maior número de safras. A seleção dos sete melhores genótipos proporcionou ganhos genéticos de 11,5% na produção de frutos, enquanto a do melhor genótipo conferiu ganho genético de 16,3%. As maiores médias genéticas preditas (>70,0 kg.pl-1 para produção de frutos foram obtidas pelos genótipos Limão-Cravo- Ipanema (1522, Santa- Bárbara-Red- Lime (884, Limão- Cravo- Limeira (863, Limão- Cravo- Taquaritinga (869, Limão- Rugoso- do -Cabo (1643, Rangpur- Rose Lime (868 e Limão- Cravo- da- Califórnia (1467. Já a acurácia seletiva da eficiência produtiva, para quatro colheitas, foi 77,4%. Para este caráter, as maiores médias genéticas (>8,0 kg.m-3 foram dos

  9. Citrus phytophtorose in humid subtropical zone of West Georgia

    Directory of Open Access Journals (Sweden)

    Mziuri Gabaidze

    2015-05-01

    Full Text Available Materials about harmfulness of phytophtorose, which is one of the significant disease of citrus, its spread, time of emergency and effective means of its control are given in the article.

  10. The draft genome of sweet orange (Citrus sinensis).

    Science.gov (United States)

    Xu, Qiang; Chen, Ling-Ling; Ruan, Xiaoan; Chen, Dijun; Zhu, Andan; Chen, Chunli; Bertrand, Denis; Jiao, Wen-Biao; Hao, Bao-Hai; Lyon, Matthew P; Chen, Jiongjiong; Gao, Song; Xing, Feng; Lan, Hong; Chang, Ji-Wei; Ge, Xianhong; Lei, Yang; Hu, Qun; Miao, Yin; Wang, Lun; Xiao, Shixin; Biswas, Manosh Kumar; Zeng, Wenfang; Guo, Fei; Cao, Hongbo; Yang, Xiaoming; Xu, Xi-Wen; Cheng, Yun-Jiang; Xu, Juan; Liu, Ji-Hong; Luo, Oscar Junhong; Tang, Zhonghui; Guo, Wen-Wu; Kuang, Hanhui; Zhang, Hong-Yu; Roose, Mikeal L; Nagarajan, Niranjan; Deng, Xiu-Xin; Ruan, Yijun

    2013-01-01

    Oranges are an important nutritional source for human health and have immense economic value. Here we present a comprehensive analysis of the draft genome of sweet orange (Citrus sinensis). The assembled sequence covers 87.3% of the estimated orange genome, which is relatively compact, as 20% is composed of repetitive elements. We predicted 29,445 protein-coding genes, half of which are in the heterozygous state. With additional sequencing of two more citrus species and comparative analyses of seven citrus genomes, we present evidence to suggest that sweet orange originated from a backcross hybrid between pummelo and mandarin. Focused analysis on genes involved in vitamin C metabolism showed that GalUR, encoding the rate-limiting enzyme of the galacturonate pathway, is significantly upregulated in orange fruit, and the recent expansion of this gene family may provide a genomic basis. This draft genome represents a valuable resource for understanding and improving many important citrus traits in the future.

  11. Breeding, genetic and genomic of citrus for disease resistance

    OpenAIRE

    Machado, Marcos A; Mariângela Cristofani-Yaly; Marinês Bastianel

    2011-01-01

    Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture regarding the phytosanitary problems. A higher number of varieties/genotypes with potential for commercial growing, either for the industry or fresh market, has been one of the main objectives of citrus breeding programs. The genetic breeding of citrus has improved, in the last decades, due to the possi...

  12. Micro-irrigation systems, automation and fertigation in citrus

    OpenAIRE

    Parameshwar Sidramappa Shirgure

    2012-01-01

    Citrus is number one group of fruits grown in more than 140 countries in the world. Micro-irrigation systems and fertigation management is one of the main concerns of the modem citrus fruit production irrespective of availability of soil, water and fertilizer resources. A variety of recommendations have emerged world over on irrigation systems and fertigation based on soil and leaf analysis of the nutrients, evapo-transpiration and water use pattern. The research review of literature has reve...

  13. Larvicidal Activity of Citrus Limonoids against Aedes Albopictus Larvae

    OpenAIRE

    Hazrat Bilal; Waseem Akram; Soaib Ali-Hassan

    2012-01-01

    Background: Development of insecticide resistance occurred due to the continuous and misuse of synthetic insecti­cides therefore, the recent study was conducted to explore eco-friendly plant extracts that have some potential to sup­press mosquito larval population.Methods: World Health Organization recommended mosquito larval bioassay method for insecticide was used while for the analysis of citrus oils for limonin and nomilin content HPLC was used.Results: Among the two citrus cultivars test...

  14. Citrus bergamia essential oil: from basic research to clinical application

    OpenAIRE

    Michele eNavarra; Carmen eMannucci; Marisa eDelbò; Gioacchino eCalapai

    2015-01-01

    Citrus bergamia Risso et Poiteau, also known as Bergamot, is a plant belonging to the Rutaceae family, defined as a hybrid of bitter orange and lemon. It is an endemic plant of the Calabria region (Italy). Bergamot fruit is primarily used for the extraction of its essential oil (bergamot essential oil: BEO), employed in perfume, cosmetics, food and confections.The aim of this review was to collect recent data from the literature on Citrus bergamia essential oil and, through a critical analysi...

  15. Citrus Fruit Intake Substantially Reduces the Risk of Esophageal Cancer

    OpenAIRE

    Wang, Anqiang; Zhu, Chengpei; Fu, Lilan; Wan, Xueshuai; Yang, Xiaobo; Zhang, Haohai; Miao, Ruoyu; He, Lian; Sang, Xinting; ZHAO, HAITAO

    2015-01-01

    Abstract Many epidemiologic studies indicate a potential association between fruit and vegetable intake and various cancers. The purpose of this meta-analysis is to investigate the association between citrus fruit intake and esophageal cancer risk. The authors conducted a comprehensive search on PubMed, EMBASE, and the Cochrane Library from inception until July 2014. Studies presenting information about citrus intake and esophageal cancer were analyzed. The authors extracted the categories of...

  16. Phenolic Compounds Characterization and Biological Activities of Citrus aurantium Bloom

    OpenAIRE

    Armin Oskoueian; Jaafar, Hawa Z. E.; Rudi Hendra; Ehsan Oskoueian; Ehsan Karimi

    2012-01-01

    Citrus plants are known to possess beneficial biological activities for human health. In addition, ethnopharmacological application of plants is a good tool to explore their bioactivities and active compounds. This research was carried out to evaluate the phenolic and flavonoid analysis, antioxidant properties, anti inflammatory and anti cancer activity of Citrus aurantium bloom. The total phenolics and flavonoids results revealed that methanolic extract contained high total phenolics and fla...

  17. Citrus growers vary in their adoption of biological control

    OpenAIRE

    Grogan, Kelly A.; Goodhue, Rachael E

    2012-01-01

    In a spring 2010 survey, we investigated the characteristics that influenced whether California growers controlled major citrus pests with beneficial insects. We also performed statistical analysis of growers' reliance on Aphytus melinus, a predatory wasp, to control California red scale. The survey results suggest that growers with greater citrus acreage and more education are more likely to use biological control. Marketing outlets, ethnicity and primary information sources also influenced ...

  18. Citrus tristeza virus-based RNAi in citrus plants induces gene silencing in Diaphorina citri, a phloem-sap sucking insect vector of citrus greening disease (Huanglongbing).

    Science.gov (United States)

    Hajeri, Subhas; Killiny, Nabil; El-Mohtar, Choaa; Dawson, William O; Gowda, Siddarame

    2014-04-20

    A transient expression vector based on Citrus tristeza virus (CTV) is unusually stable. Because of its stability it is being considered for use in the field to control Huanglongbing (HLB), which is caused by Candidatus Liberibacter asiaticus (CLas) and vectored by Asian citrus psyllid, Diaphorina citri. In the absence of effective control strategies for CLas, emphasis has been on control of D. citri. Coincident cohabitation in phloem tissue by CLas, D. citri and CTV was exploited to develop a novel method to mitigate HLB through RNA interference (RNAi). Since CTV has three RNA silencing suppressors, it was not known if CTV-based vector could induce RNAi in citrus. Yet, expression of sequences targeting citrus phytoene desaturase gene by CTV-RNAi resulted in photo-bleaching phenotype. CTV-RNAi vector, engineered with truncated abnormal wing disc (Awd) gene of D. citri, induced altered Awd expression when silencing triggers ingested by feeding D. citri nymphs. Decreased Awd in nymphs resulted in malformed-wing phenotype in adults and increased adult mortality. This impaired ability of D. citri to fly would potentially limit the successful vectoring of CLas bacteria between citrus trees in the grove. CTV-RNAi vector would be relevant for fast-track screening of candidate sequences for RNAi-mediated pest control.

  19. Citrus tristeza virus-based RNAi in citrus plants induces gene silencing in Diaphorina citri, a phloem-sap sucking insect vector of citrus greening disease (Huanglongbing).

    Science.gov (United States)

    Hajeri, Subhas; Killiny, Nabil; El-Mohtar, Choaa; Dawson, William O; Gowda, Siddarame

    2014-04-20

    A transient expression vector based on Citrus tristeza virus (CTV) is unusually stable. Because of its stability it is being considered for use in the field to control Huanglongbing (HLB), which is caused by Candidatus Liberibacter asiaticus (CLas) and vectored by Asian citrus psyllid, Diaphorina citri. In the absence of effective control strategies for CLas, emphasis has been on control of D. citri. Coincident cohabitation in phloem tissue by CLas, D. citri and CTV was exploited to develop a novel method to mitigate HLB through RNA interference (RNAi). Since CTV has three RNA silencing suppressors, it was not known if CTV-based vector could induce RNAi in citrus. Yet, expression of sequences targeting citrus phytoene desaturase gene by CTV-RNAi resulted in photo-bleaching phenotype. CTV-RNAi vector, engineered with truncated abnormal wing disc (Awd) gene of D. citri, induced altered Awd expression when silencing triggers ingested by feeding D. citri nymphs. Decreased Awd in nymphs resulted in malformed-wing phenotype in adults and increased adult mortality. This impaired ability of D. citri to fly would potentially limit the successful vectoring of CLas bacteria between citrus trees in the grove. CTV-RNAi vector would be relevant for fast-track screening of candidate sequences for RNAi-mediated pest control. PMID:24572372

  20. Pathogen infection drives patterns of nutrient resorption in citrus plants.

    Science.gov (United States)

    Cao, Jirong; Cheng, Chunzhen; Yang, Junjie; Wang, Qibing

    2015-01-01

    Nutrient resorption processes in the plants infected by pathogen remain poorly understood. Huanglongbing (HLB) is a destructive disease of citrus. HLB-pathogen 'Candidatus Liberibacter asiaticus' grows specifically in the phloem of hosts and may cause problems in the plant vascular system after infection. Therefore, it brings a great concern about the phloem nutrient transport and nutrient intra-cycling in HLB-affected plants. We investigated the effects of 'Ca. L. asiaticus' infection on nitrogen (N) and phosphorus (P) concentrations and resorption in different citrus species (i.e. Citrus reticulata, Citrus limon and Citrus maxima). HLB-pathogen infection had distinctive impacts on nutrient resorption in different species. P resorption efficiency substantially decreased in infected C. reticulata plants relative to the healthy plants in summer, which may account for the marked decrease in the average fruit yield. P resorption was more efficient in infected C. limon plants than in the healthy plants. However, for C. maxima plants, HLB had no significant effects on N:P ratio in live leaves and resorption efficiency as well as on fruit yield. Keeping efficient internal nutrient cycling can be a strategy of citrus species being tolerant to HLB. PMID:26419510

  1. An RNA-Seq-based reference transcriptome for Citrus.

    Science.gov (United States)

    Terol, Javier; Tadeo, Francisco; Ventimilla, Daniel; Talon, Manuel

    2016-03-01

    Previous RNA-Seq studies in citrus have been focused on physiological processes relevant to fruit quality and productivity of the major species, especially sweet orange. Less attention has been paid to vegetative or reproductive tissues, while most Citrus species have never been analysed. In this work, we characterized the transcriptome of vegetative and reproductive tissues from 12 Citrus species from all main phylogenetic groups. Our aims were to acquire a complete view of the citrus transcriptome landscape, to improve previous functional annotations and to obtain genetic markers associated with genes of agronomic interest. 28 samples were used for RNA-Seq analysis, obtained from 12 Citrus species: C. medica, C. aurantifolia, C. limon, C. bergamia, C. clementina, C. deliciosa, C. reshni, C. maxima, C. paradisi, C. aurantium, C. sinensis and Poncirus trifoliata. Four different organs were analysed: root, phloem, leaf and flower. A total of 3421 million Illumina reads were produced and mapped against the reference C. clementina genome sequence. Transcript discovery pipeline revealed 3326 new genes, the number of genes with alternative splicing was increased to 19,739, and a total of 73,797 transcripts were identified. Differential expression studies between the four tissues showed that gene expression is overall related to the physiological function of the specific organs above any other variable. Variants discovery analysis revealed the presence of indels and SNPs in genes associated with fruit quality and productivity. Pivotal pathways in citrus such as those of flavonoids, flavonols, ethylene and auxin were also analysed in detail.

  2. Physicochemical characteristics of citrus seed oils from kerman, iran.

    Science.gov (United States)

    Reazai, Mohammad; Mohammadpourfard, Issa; Nazmara, Shahrokh; Jahanbakhsh, Mahdi; Shiri, Leila

    2014-01-01

    Recently, there has been a great deal of attention on usage, byproducts, and wastes of the food industry. There have been many studies on the properties of citrus seeds and extracted oil from citrus grown in Kerman, Iran. The rate of oil content of citrus seeds varies between 33.4% and 41.9%. Linoleic acid (33.2% to 36.3%) is the key fatty acid found in citrus seeds oil and oleic (24.8% to 29.3%) and palmitic acids (23.5% to 29.4%) are the next main fatty acids, respectively. There are also other acids found at trivial rates such as stearic, palmitoleic, and linolenic. With variation between 0.54 meg/kg and 0.77 mgq/kg in peroxide values of citrus seed oils, acidity value of the oil varies between 0.44% and 0.72%. The results of the study showed that citrus seeds under study (orange and sour lemon grown in Kerman province) and the extracted oil have the potential of being used as the source of edible oil. PMID:25136460

  3. Physicochemical Characteristics of Citrus Seed Oils from Kerman, Iran

    Directory of Open Access Journals (Sweden)

    Mohammad Reazai

    2014-01-01

    Full Text Available Recently, there has been a great deal of attention on usage, byproducts, and wastes of the food industry. There have been many studies on the properties of citrus seeds and extracted oil from citrus grown in Kerman, Iran. The rate of oil content of citrus seeds varies between 33.4% and 41.9%. Linoleic acid (33.2% to 36.3% is the key fatty acid found in citrus seeds oil and oleic (24.8% to 29.3% and palmitic acids (23.5% to 29.4% are the next main fatty acids, respectively. There are also other acids found at trivial rates such as stearic, palmitoleic, and linolenic. With variation between 0.54 meg/kg and 0.77 mgq/kg in peroxide values of citrus seed oils, acidity value of the oil varies between 0.44% and 0.72%. The results of the study showed that citrus seeds under study (orange and sour lemon grown in Kerman province and the extracted oil have the potential of being used as the source of edible oil.

  4. An RNA-Seq-based reference transcriptome for Citrus.

    Science.gov (United States)

    Terol, Javier; Tadeo, Francisco; Ventimilla, Daniel; Talon, Manuel

    2016-03-01

    Previous RNA-Seq studies in citrus have been focused on physiological processes relevant to fruit quality and productivity of the major species, especially sweet orange. Less attention has been paid to vegetative or reproductive tissues, while most Citrus species have never been analysed. In this work, we characterized the transcriptome of vegetative and reproductive tissues from 12 Citrus species from all main phylogenetic groups. Our aims were to acquire a complete view of the citrus transcriptome landscape, to improve previous functional annotations and to obtain genetic markers associated with genes of agronomic interest. 28 samples were used for RNA-Seq analysis, obtained from 12 Citrus species: C. medica, C. aurantifolia, C. limon, C. bergamia, C. clementina, C. deliciosa, C. reshni, C. maxima, C. paradisi, C. aurantium, C. sinensis and Poncirus trifoliata. Four different organs were analysed: root, phloem, leaf and flower. A total of 3421 million Illumina reads were produced and mapped against the reference C. clementina genome sequence. Transcript discovery pipeline revealed 3326 new genes, the number of genes with alternative splicing was increased to 19,739, and a total of 73,797 transcripts were identified. Differential expression studies between the four tissues showed that gene expression is overall related to the physiological function of the specific organs above any other variable. Variants discovery analysis revealed the presence of indels and SNPs in genes associated with fruit quality and productivity. Pivotal pathways in citrus such as those of flavonoids, flavonols, ethylene and auxin were also analysed in detail. PMID:26261026

  5. EFEITOS DE GIBERELINAS, CITOCININAS E DO NITRATO DE POTÁSSIO NO CRESCIMENTO E DESENVOLVIMENTO DO PORTA-ENXERTO DE LIMOEIRO `CRAVO'

    Directory of Open Access Journals (Sweden)

    S. LEONEL

    1996-05-01

    Full Text Available Os efeitos de reguladores vegetais do grupo das giberelinas e citocininas, bem como do nitrato de potássio, foram estudados no crescimento e desenvolvimento do porta-enxerto de limoeiro `Cravo' (Citrus limonia Osbeck. As sementes foram extraídas de frutos maduros, lavadas, secas à sombra e armazenadas em câmara fria (4 - 5oC durante sessenta dias, até o mês de maio/1993, quando foram semeadas nos canteiros de semeadura. As pulverizações com os fitorreguladores foram realizadas no viveiro e tiveram início com as plantas aos 8 meses de idade, contados a partir da semeadura, sendo efetuadas um total de 4 pulverizações, realizadas a intervalos quinzenais. Os tratamentos corresponderam a: 25, 50 e 75 ppm de GA3; 25, 50 e 75 ppm de GA4+GA7+fenilmetil-aminopurina; 20 ppm de fenilmetilpiranil-aminopurina; 0,2% de KNO3 e testemunha (sem pulverização. Os resultados obtidos permitiram concluir que as pulverizações com os reguladores vegetais e com o nitrato de potássio, realizadas no período de janeiro a março (verão, não tiveram efeito na diminuição do tempo de formação das plantas jovens de limoeiro `Cravo', tendo inclusive, o tratamento com KNO3 0,2%, exercido efeito depressivo no desenvolvimento das mesmas.The effects of gibberellins, cytokinins and potassium nitrate on the development of `Rangpur'lime (Citrus limonia Osbeck rootstocks were studied. Seeds from ripe fruits were washed, dried and stored at 4 - 5o C for 60 days. They were planted in nursery plots in May/1993 and when the seedlings had 10 - 20 cm height, they were transplanted to a second nursery stage. The seedlings were sprayed at 15-day intervals, with plant regulators and potassium nitrate, after reaching the age of 8 months. From January to March/1993, the seedlings were sprayed with: 25, 50 and 75 ppm of GA3; 25, 50 and 75 ppm of GA4+7 + phenylmethyl-aminepurine; 20 ppm of phenylmethylpyranil-aminepurine; 0.2% of KNO3. The seedlings planted in May/1993 did not

  6. Desenvolvimento vegetativo, produção e qualidade de frutos da tangerina 'Fremont' sobre quatro porta-enxertos Growth, yield and fruit quality of 'Fremont' mandarin on four rootstocks

    Directory of Open Access Journals (Sweden)

    Erick Espinoza Núñez

    2007-08-01

    Full Text Available Com o objetivo de avaliar o desenvolvimento vegetativo, produção e qualidade de frutos da tangerina 'Fremont' (C. clementina Hort. ex Tan. X C. reticulata Blanco sobre os porta-enxertos limão 'Cravo' (C. limonia Osbeck, citrumelo 'Swingle' (P. trifoliata Raf. x C. paradisi Macf., tangerina 'Cleópatra' (Citrus reshni Hort. ex Tan. e tangelo 'Orlando' (C. reticulata Blanco x C. paradisi Macf., foi instalado um experimento em Bebedouro-SP, em 1997. Embora diferenças na produção tenham sido registradas em função dos porta-enxertos, nos anos de 2003 a 2005, a produção acumulada nas safras de 2000 a 2006 não revelou influência dos mesmos. Os valores do índice de alternância de produção e eficiência de produção não foram influenciados pelos porta-enxertos. O teor de sólidos solúveis, bem como acidez total foram superiores nos frutos das plantas enxertadas sobre citrumelo 'Swingle' e tangerina 'Cleópatra'. Os valores de volume da copa e diâmetro do tronco foram superiores nas árvores sobre tangelo 'Orlando' e tangerina 'Cleópatra'.An experiment was installed in Bebedouro, SP, Brazil in 1997, with the aim of evaluate the effect of the rootstocks 'Rangpur' lime (C. limonia Osbeck, 'Swingle' citrumelo (P. trifoliata Raf. x C. paradisi Macf., 'Cleopatra' mandarin (Citrus reshni Hort. ex Tan. and 'Orlando' tangelo (C. reticulata Blanco x C. paradisi Macf. on plant growth, yield and fruit quality of 'Fremont' mandarin (C. clementina Hort. ex Tan. X C. reticulata Blanco. Despite that differences in yield have been registered related to the rootstocks between 2003 to 2005; cumulative yield from 2000 to 2006 seasons was not affected by the rootstock. Alternate bearing index and yield efficiency values were not influenced by the rootstocks. Fruits from trees on 'Swingle' citrumelo and 'Cleópatra' mandarin had higher values of total soluble solids concentration and total acids. Trunk diameter and canopy volume values were higher in

  7. Generating Asian citrus Psyllid Diaphorina citri Kuwayama (Homoptera: Psyllidae) with twisting wings to prevent the spread of citrus greening disease

    OpenAIRE

    El-Shesheny, I.; Harjeri, S.; Gowda, S.; Killiny, N.

    2014-01-01

    Huanglongbing (HLB) is seriously threatening and causing considerable economic losses to the citrus groves. Its Management depends critically on the control of the Asian citrus Psyllid (ACP), the vector of the cause of HLB, Candidatus Liberibacter asiaticus bacteria (CLas). Silencing genes by RNA interference (RNAi) is a promising technique to control pests. In this study, the abnormal disk wing (awd) has been selected from the available psyllid annotated genome. It has been known that awd ge...

  8. Antimicrobial Nanoemulsion Formulation with Improved Penetration of Foliar Spray through Citrus Leaf Cuticles to Control Citrus Huanglongbing.

    Directory of Open Access Journals (Sweden)

    Chuanyu Yang

    Full Text Available Huanglongbing (HLB is the most serious disease affecting the citrus industry worldwide to date. The causal agent, Candidatus Liberibacter asiaticus (Las, resides in citrus phloem, which makes it difficult to effectively treat with chemical compounds. In this study, a transcuticular nanoemulsion formulation was developed to enhance the permeation of an effective antimicrobial compound (ampicillin; Amp against HLB disease through the citrus cuticle into the phloem via a foliar spray. The results demonstrated that efficiency of cuticle isolation using an enzymatic method (pectinase and cellulase was dependent on the citrus cultivar and Las-infection, and it was more difficult to isolate cuticles from valencia orange (Citrus sinensis and HLB-symptomatic leaves. Of eight adjuvants tested, Brij 35 provided the greatest increase in permeability of the HLB-affected cuticle with a 3.33-fold enhancement of cuticular permeability over water control. An in vitro assay using Bacillus subtilis showed that nanoemulsion formulations containing Amp (droplets size = 5.26 ± 0.04 nm and 94 ± 1.48 nm coupled with Brij 35 resulted in greater inhibitory zone diameters (5.75 mm and 6.66 mm compared to those of Brij 35 (4.34 mm and Amp solution (2.83 mm alone. Furthermore, the nanoemulsion formulations eliminated Las bacteria in HLB-affected citrus in planta more efficiently than controls. Our study shows that a water in oil (W/O nanoemulsion formulation may provide a useful model for the effective delivery of chemical compounds into citrus phloem via a foliar spray for controlling citrus HLB.

  9. Antimicrobial Nanoemulsion Formulation with Improved Penetration of Foliar Spray through Citrus Leaf Cuticles to Control Citrus Huanglongbing.

    Science.gov (United States)

    Yang, Chuanyu; Powell, Charles A; Duan, Yongping; Shatters, Robert; Zhang, Muqing

    2015-01-01

    Huanglongbing (HLB) is the most serious disease affecting the citrus industry worldwide to date. The causal agent, Candidatus Liberibacter asiaticus (Las), resides in citrus phloem, which makes it difficult to effectively treat with chemical compounds. In this study, a transcuticular nanoemulsion formulation was developed to enhance the permeation of an effective antimicrobial compound (ampicillin; Amp) against HLB disease through the citrus cuticle into the phloem via a foliar spray. The results demonstrated that efficiency of cuticle isolation using an enzymatic method (pectinase and cellulase) was dependent on the citrus cultivar and Las-infection, and it was more difficult to isolate cuticles from valencia orange (Citrus sinensis) and HLB-symptomatic leaves. Of eight adjuvants tested, Brij 35 provided the greatest increase in permeability of the HLB-affected cuticle with a 3.33-fold enhancement of cuticular permeability over water control. An in vitro assay using Bacillus subtilis showed that nanoemulsion formulations containing Amp (droplets size = 5.26 ± 0.04 nm and 94 ± 1.48 nm) coupled with Brij 35 resulted in greater inhibitory zone diameters (5.75 mm and 6.66 mm) compared to those of Brij 35 (4.34 mm) and Amp solution (2.83 mm) alone. Furthermore, the nanoemulsion formulations eliminated Las bacteria in HLB-affected citrus in planta more efficiently than controls. Our study shows that a water in oil (W/O) nanoemulsion formulation may provide a useful model for the effective delivery of chemical compounds into citrus phloem via a foliar spray for controlling citrus HLB. PMID:26207823

  10. Morphological, molecular and virulence characterization of three Lencanicillium species infecting Asian citrus psyllids in Huangyan citrus groves.

    Science.gov (United States)

    Lu, Lianming; Cheng, Baoping; Du, Danchao; Hu, Xiurong; Peng, Aitian; Pu, Zhanxu; Zhang, Xiaoya; Huang, Zhendong; Chen, Guoqing

    2015-02-01

    Citrus greening or Huanglongbing (HLB) is caused by the infection of Candidatus Liberibacter spp. in citrus plants. Since Asian citrus psyllid is the primary vector of this bacterial pathogen, the spread of HLB can be mitigated by suppressing Asian citrus psyllid populations in citrus groves using entomopathogens. To expand the current data on entomopathogens infecting Asian citrus psyllids, we isolated and characterized three different entomopathogens. Strains ZJLSP07, ZJLA08, and ZJLP09 infected the Asian citrus psyllid, Diaphorina citri Kuwayama, in Huangyan citrus groves. Based on molecular and morphological analyses, two were identified as Lecanicillium attenuatum and Lecanicillium psalliotae, and the third was recognized as an unidentified species of the genus, Lecanicillium. The corrected mortalities caused by strains ZJLSP07, ZJLA08 were 100% at 7days post-inoculation, while by ZJLP09 complete mortality occurred at 6days after inoculation, with 1.0×10(8)conidia/ml at 25°C and a relative humidity of 90% in the laboratory. Under the same condition, the corrected mortalities caused by strains ZJLSP07, ZJLA08 and ZJLP09 were 100%, 92.55% and 100%, respectively at 9days post-inoculation in the greenhouse. Our findings also revealed that these fungal strains infected D. citri using hyphae that penetrated deep into the insect tissues. Further, all three strains secreted the enzymes proteinases, chitinases and lipases with a potential to destroy insect tissues. Interestingly, strain ZJLP09 had an earlier invasion time and the highest levels of enzyme activities when compared to the other two strains. These findings have expanded the existing pool of entomopathogenic fungi that infect D. citri and can be potentially used for the management of D. citri populations. PMID:25593036

  11. The Distribution of Coumarins and Furanocoumarins in Citrus Species Closely Matches Citrus Phylogeny and Reflects the Organization of Biosynthetic Pathways

    Science.gov (United States)

    Dugrand-Judek, Audray; Olry, Alexandre; Hehn, Alain; Costantino, Gilles; Ollitrault, Patrick; Froelicher, Yann; Bourgaud, Frédéric

    2015-01-01

    Citrus plants are able to produce defense compounds such as coumarins and furanocoumarins to cope with herbivorous insects and pathogens. In humans, these chemical compounds are strong photosensitizers and can interact with medications, leading to the “grapefruit juice effect”. Removing coumarins and furanocoumarins from food and cosmetics imply additional costs and might alter product quality. Thus, the selection of Citrus cultivars displaying low coumarin and furanocoumarin contents constitutes a valuable alternative. In this study, we performed ultra-performance liquid chromatography coupled with mass spectrometry analyses to determine the contents of these compounds within the peel and the pulp of 61 Citrus species representative of the genetic diversity all Citrus. Generally, Citrus peel contains larger diversity and higher concentrations of coumarin/furanocoumarin than the pulp of the same fruits. According to the chemotypes found in the peel, Citrus species can be separated into 4 groups that correspond to the 4 ancestral taxa (pummelos, mandarins, citrons and papedas) and extended with their respective secondary species descendants. Three of the 4 ancestral taxa (pummelos, citrons and papedas) synthesize high amounts of these compounds, whereas mandarins appear practically devoid of them. Additionally, all ancestral taxa and their hybrids are logically organized according to the coumarin and furanocoumarin pathways described in the literature. This organization allows hypotheses to be drawn regarding the biosynthetic origin of compounds for which the biogenesis remains unresolved. Determining coumarin and furanocoumarin contents is also helpful for hypothesizing the origin of Citrus species for which the phylogeny is presently not firmly established. Finally, this work also notes favorable hybridization schemes that will lead to low coumarin and furanocoumarin contents, and we propose to select mandarins and Ichang papeda as Citrus varieties for use in

  12. Morphological, molecular and virulence characterization of three Lencanicillium species infecting Asian citrus psyllids in Huangyan citrus groves.

    Science.gov (United States)

    Lu, Lianming; Cheng, Baoping; Du, Danchao; Hu, Xiurong; Peng, Aitian; Pu, Zhanxu; Zhang, Xiaoya; Huang, Zhendong; Chen, Guoqing

    2015-02-01

    Citrus greening or Huanglongbing (HLB) is caused by the infection of Candidatus Liberibacter spp. in citrus plants. Since Asian citrus psyllid is the primary vector of this bacterial pathogen, the spread of HLB can be mitigated by suppressing Asian citrus psyllid populations in citrus groves using entomopathogens. To expand the current data on entomopathogens infecting Asian citrus psyllids, we isolated and characterized three different entomopathogens. Strains ZJLSP07, ZJLA08, and ZJLP09 infected the Asian citrus psyllid, Diaphorina citri Kuwayama, in Huangyan citrus groves. Based on molecular and morphological analyses, two were identified as Lecanicillium attenuatum and Lecanicillium psalliotae, and the third was recognized as an unidentified species of the genus, Lecanicillium. The corrected mortalities caused by strains ZJLSP07, ZJLA08 were 100% at 7days post-inoculation, while by ZJLP09 complete mortality occurred at 6days after inoculation, with 1.0×10(8)conidia/ml at 25°C and a relative humidity of 90% in the laboratory. Under the same condition, the corrected mortalities caused by strains ZJLSP07, ZJLA08 and ZJLP09 were 100%, 92.55% and 100%, respectively at 9days post-inoculation in the greenhouse. Our findings also revealed that these fungal strains infected D. citri using hyphae that penetrated deep into the insect tissues. Further, all three strains secreted the enzymes proteinases, chitinases and lipases with a potential to destroy insect tissues. Interestingly, strain ZJLP09 had an earlier invasion time and the highest levels of enzyme activities when compared to the other two strains. These findings have expanded the existing pool of entomopathogenic fungi that infect D. citri and can be potentially used for the management of D. citri populations.

  13. Living on the Edges: Spatial Niche Occupation of Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), in Citrus Groves

    OpenAIRE

    Mamoudou Sétamou; David W Bartels

    2015-01-01

    The spatial niche occupation of the Asian citrus psyllid, Diaphorina citri Kuwayama, 1908, was evaluated to determine its field colonization and food resource exploitation strategies in citrus groves. Mature grapefruit and sweet orange groves were surveyed as part of an area-wide program in 2009-2010 to determine D. citri population densities and between-tree distribution. In both cultivars, significantly more psyllids were found on perimeter trees throughout the study period suggesting a str...

  14. Antimicrobial Nanoemulsion Formulation with Improved Penetration of Foliar Spray through Citrus Leaf Cuticles to Control Citrus Huanglongbing

    Science.gov (United States)

    Yang, Chuanyu; Powell, Charles A.; Duan, Yongping; Shatters, Robert; Zhang, Muqing

    2015-01-01

    Huanglongbing (HLB) is the most serious disease affecting the citrus industry worldwide to date. The causal agent, Candidatus Liberibacter asiaticus (Las), resides in citrus phloem, which makes it difficult to effectively treat with chemical compounds. In this study, a transcuticular nanoemulsion formulation was developed to enhance the permeation of an effective antimicrobial compound (ampicillin; Amp) against HLB disease through the citrus cuticle into the phloem via a foliar spray. The results demonstrated that efficiency of cuticle isolation using an enzymatic method (pectinase and cellulase) was dependent on the citrus cultivar and Las-infection, and it was more difficult to isolate cuticles from valencia orange (Citrus sinensis) and HLB-symptomatic leaves. Of eight adjuvants tested, Brij 35 provided the greatest increase in permeability of the HLB-affected cuticle with a 3.33-fold enhancement of cuticular permeability over water control. An in vitro assay using Bacillus subtilis showed that nanoemulsion formulations containing Amp (droplets size = 5.26 ± 0.04 nm and 94 ± 1.48 nm) coupled with Brij 35 resulted in greater inhibitory zone diameters (5.75 mm and 6.66 mm) compared to those of Brij 35 (4.34 mm) and Amp solution (2.83 mm) alone. Furthermore, the nanoemulsion formulations eliminated Las bacteria in HLB-affected citrus in planta more efficiently than controls. Our study shows that a water in oil (W/O) nanoemulsion formulation may provide a useful model for the effective delivery of chemical compounds into citrus phloem via a foliar spray for controlling citrus HLB. PMID:26207823

  15. Pharmacognostical evaluation of Citrus jambhiri Lush. fruit

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    Swapnil Y Chaudhari

    2014-01-01

    Full Text Available Background: Citrus jambhiri Lush., commonly known as Jambīra Nimbū in Sanskrit is medium to large indigenous tree with spreading habit, less spiny than lemon and belonging to the family Rutaceae. In Ayurveda, it is used in many pharmaceutical procedures of purification (Śodhana, calcination (Māraṇa etc., Though it is an important plant, till date, no pharmacognostical reports have been available on its fruit. Materials and Methods: Study of fruit and its powder, histochemical tests and preliminary physicochemical investigations were done. Results and Conclusion: Results showed prismatic crystals of calcium oxalate, aerenchyma cells, oil globules, pitted vessels, scalariform vessels, juicy sac, etc., Preliminary physicochemical analysis revealed loss on drying (1.1%, ash value (1.4%, alcohol soluble extract (28.6%, and water soluble extract (53.3%. These observations can be of use in future studies.

  16. Isolation, characterization and modification of citrus pectins

    Directory of Open Access Journals (Sweden)

    MARIA KRATCHANOVA

    2012-01-01

    Full Text Available Orange and lemon peels were used for obtaining pectic polysaccharides. Citrus peels were previously treated with 96% ethanol, and the obtained alcohol-insoluble solids (AIS were subjected to a sequential extraction with hot distilled water and hot 0.5% HCl. Water- and acid-extracted orange (WEOP and AEOP and lemon (WELP and AELP pectins were obtained. Acid extraction gave higher yields of pectin than water extraction and lemon peels were richer in pectin. Comparative investigations were carried out with chromatographically purified commercial citrus pectin (CPCP. Chemical and physicochemical characterization of all pectins was accomplished. It was found that pectins were similar in anhydrouronic acid content (AUАC, 69-81%, but differed in their degree of methylesterification (DM, 55-81%. Generally water-extracted pectins were with higher DM. Both orange pectins were with higher DM and degree of acetylation (DA, 2%, in comparison with the corresponding lemon pectins. Water-extracted pectins were with higher degree of feruloylation (DF, 0.12-0.34%. To our knowledge this is the first report on the estimation of ester-linked ferulic acid in orange and lemon peel pectins. Pectic polysaccharides differed in molecular weight and homogeneity. WELP was with the highest molecular weight and homogeneity. The pectins contained D-galacturonic and D-glucuronic acids, L-arabinose, D-galactose, L-fucose, L-rhamnose and D-xylose. All investigated pectins showed immunostimulating activity by complement activation in the classical pathway at 1.25 and 2.5 mg/mL. Pectic polysaccharides were modified with endopolygalacturonase. Enzyme-modified CPCP and WEOP had higher anti-complementary activity than the corresponding initial pectins.

  17. Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication.

    Science.gov (United States)

    Wu, G Albert; Prochnik, Simon; Jenkins, Jerry; Salse, Jerome; Hellsten, Uffe; Murat, Florent; Perrier, Xavier; Ruiz, Manuel; Scalabrin, Simone; Terol, Javier; Takita, Marco Aurélio; Labadie, Karine; Poulain, Julie; Couloux, Arnaud; Jabbari, Kamel; Cattonaro, Federica; Del Fabbro, Cristian; Pinosio, Sara; Zuccolo, Andrea; Chapman, Jarrod; Grimwood, Jane; Tadeo, Francisco R; Estornell, Leandro H; Muñoz-Sanz, Juan V; Ibanez, Victoria; Herrero-Ortega, Amparo; Aleza, Pablo; Pérez-Pérez, Julián; Ramón, Daniel; Brunel, Dominique; Luro, François; Chen, Chunxian; Farmerie, William G; Desany, Brian; Kodira, Chinnappa; Mohiuddin, Mohammed; Harkins, Tim; Fredrikson, Karin; Burns, Paul; Lomsadze, Alexandre; Borodovsky, Mark; Reforgiato, Giuseppe; Freitas-Astúa, Juliana; Quetier, Francis; Navarro, Luis; Roose, Mikeal; Wincker, Patrick; Schmutz, Jeremy; Morgante, Michele; Machado, Marcos Antonio; Talon, Manuel; Jaillon, Olivier; Ollitrault, Patrick; Gmitter, Frederick; Rokhsar, Daniel

    2014-07-01

    Cultivated citrus are selections from, or hybrids of, wild progenitor species whose identities and contributions to citrus domestication remain controversial. Here we sequence and compare citrus genomes--a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-orange genomes--and show that cultivated types derive from two progenitor species. Although cultivated pummelos represent selections from one progenitor species, Citrus maxima, cultivated mandarins are introgressions of C. maxima into the ancestral mandarin species Citrus reticulata. The most widely cultivated citrus, sweet orange, is the offspring of previously admixed individuals, but sour orange is an F1 hybrid of pure C. maxima and C. reticulata parents, thus implying that wild mandarins were part of the early breeding germplasm. A Chinese wild 'mandarin' diverges substantially from C. reticulata, thus suggesting the possibility of other unrecognized wild citrus species. Understanding citrus phylogeny through genome analysis clarifies taxonomic relationships and facilitates sequence-directed genetic improvement. PMID:24908277

  18. Antimicrobial Activity and Phytochemical Analysis of Citrus Fruit Peels -Utilization of Fruit Waste

    Directory of Open Access Journals (Sweden)

    K. Ashok kumar

    2011-06-01

    Full Text Available Antibacterial activity of five different solvent extracts(ethyl acetate, acetone, ethanol, petroleum ether and water prepared by soxhlet extractor from two citrus fruit peel (Citrus sinensis and Citrus limon were screened against five pathogenic bacteria Staphylococcus aureus, Bacillus subtilis, Escherichia coli , Klebsiella pneumonia and Salmonella typhi. The highest antibacterial potentiality was exhibited by the acetone peel extract of Citrus sinensis followed by the ethyl acetate peel extract of Citrus limon. The peel extract of Citrus sinensis and Citrus limon can be considered to be as equally potent as the antibiotics, such as metacillin and penicillin. MICs were tested at concentrations ranging from 50-6.25 mg/ml as wells as their MBCs. The phytochemical analysis of the citrus peel extracts showed the presence of flavonoids, saponins, steroids, terpenoids, tannins and alkaloids

  19. Characterization of an ATP/ADP translocase in the citrus huanglongbing bacterium, Candidatus Liberibacter

    Science.gov (United States)

    Citrus huanglongbing (HLB), a disease currently threatening the citrus industry worldwide, has been associated with three different species of Alphaproteobacteria known as Candidatus Liberibacter. A complete genome sequence was recently obtained via metagenomics for Ca. L. asiaticus (Las), the prom...

  20. Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication.

    Science.gov (United States)

    Wu, G Albert; Prochnik, Simon; Jenkins, Jerry; Salse, Jerome; Hellsten, Uffe; Murat, Florent; Perrier, Xavier; Ruiz, Manuel; Scalabrin, Simone; Terol, Javier; Takita, Marco Aurélio; Labadie, Karine; Poulain, Julie; Couloux, Arnaud; Jabbari, Kamel; Cattonaro, Federica; Del Fabbro, Cristian; Pinosio, Sara; Zuccolo, Andrea; Chapman, Jarrod; Grimwood, Jane; Tadeo, Francisco R; Estornell, Leandro H; Muñoz-Sanz, Juan V; Ibanez, Victoria; Herrero-Ortega, Amparo; Aleza, Pablo; Pérez-Pérez, Julián; Ramón, Daniel; Brunel, Dominique; Luro, François; Chen, Chunxian; Farmerie, William G; Desany, Brian; Kodira, Chinnappa; Mohiuddin, Mohammed; Harkins, Tim; Fredrikson, Karin; Burns, Paul; Lomsadze, Alexandre; Borodovsky, Mark; Reforgiato, Giuseppe; Freitas-Astúa, Juliana; Quetier, Francis; Navarro, Luis; Roose, Mikeal; Wincker, Patrick; Schmutz, Jeremy; Morgante, Michele; Machado, Marcos Antonio; Talon, Manuel; Jaillon, Olivier; Ollitrault, Patrick; Gmitter, Frederick; Rokhsar, Daniel

    2014-07-01

    Cultivated citrus are selections from, or hybrids of, wild progenitor species whose identities and contributions to citrus domestication remain controversial. Here we sequence and compare citrus genomes--a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-orange genomes--and show that cultivated types derive from two progenitor species. Although cultivated pummelos represent selections from one progenitor species, Citrus maxima, cultivated mandarins are introgressions of C. maxima into the ancestral mandarin species Citrus reticulata. The most widely cultivated citrus, sweet orange, is the offspring of previously admixed individuals, but sour orange is an F1 hybrid of pure C. maxima and C. reticulata parents, thus implying that wild mandarins were part of the early breeding germplasm. A Chinese wild 'mandarin' diverges substantially from C. reticulata, thus suggesting the possibility of other unrecognized wild citrus species. Understanding citrus phylogeny through genome analysis clarifies taxonomic relationships and facilitates sequence-directed genetic improvement.

  1. Obtenção de híbridos somáticos de limão 'Cravo' e tangerina 'Cleópatra' Somatic hybridization between 'Rangpur' lime and 'Cleópatra' mandarin

    Directory of Open Access Journals (Sweden)

    Rodrigo Rocha Latado

    2002-12-01

    Full Text Available Este trabalho teve como objetivo a obtenção de híbridos somáticos entre o limão 'Cravo' (Citrus limonia Osbeck e a tangerina 'Cleópatra' (Citrus reshni Hort., para serem usados como porta-enxertos de citros. O limão 'Cravo' é atualmente o principal porta-enxerto comercial utilizado no Brasil, em virtude de suas boas qualidades agronômicas. Entretanto, é suscetível ao "declínio" dos citros, doença responsável pela eliminação anual de milhões de plantas cítricas no Brasil. A tangerina 'Cleópatra' é uma espécie bastante utilizada como porta-enxerto em outros países e tem sido descrita na literatura como tolerante ao "declínio". Protoplastos de suspensões celulares embriogênicas e protoplastos derivados de tecidos foliares foram utilizados para fusão com solução de PEG (50% e posterior cultivo em agarose. A porcentagem de obtenção de células híbridas interespecíficas logo após a fusão, variou entre 5,1% e 6,8%. Foram obtidas mais de 500 plantas a partir de produtos de fusão cultivados em gotas de agarose. No total de 180 plantas avaliadas, 11 híbridos somáticos foram discriminados e confirmados, utilizando-se marcadores moleculares RAPD e isoenzimáticos (sistemas PO, IDH e PGI. Seis plantas foram aclimatizadas, plantadas no solo e estão sendo multiplicadas por estaquia para avaliação futura como porta-enxertos de citros.The aim of this work was to obtain somatic hybrids between 'Rangpur' lime (Citrus limonia Osbeck and 'Cleópatra' mandarin (Citrus reshni Hort., to be used as a citrus rootstock. 'Rangpur' lime is the most important Brazilian citrus rootstock due to its higher horticultural performance. Nevertheless, this species is susceptible to blight disease, which is responsible for the death of millions of productive trees per year in Brazil. 'Cleópatra' mandarin has become an increasingly important rootstock in other countries and has been reported as being tolerant to citrus blight disease. Leaf

  2. Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication

    OpenAIRE

    Wu, G. Albert; Prochnik, Simon; Jenkins, Jerry; Salse, Jerome; Hellsten, Uffe; Murat, Florent; Perrier, Xavier; Ruiz, Manuel; Scalabrin, Simone; Terol, Javier; Takita, Marco Aurelio; Labadie, Karine; Poulain, Julie; Couloux, Arnaud; Jabbari, Kamel

    2014-01-01

    Cultivated citrus are selections from, or hybrids of, wild progenitor species whose identities and contributions to citrus domestication remain controversial. Here we sequence and compare citrus genomes-a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-orange genomes- and show that cultivated types derive from two progenitor species. Although cultivated pummelos represent selections from one progenitor species, Citrus maxima, cultivated mandarins ...

  3. Genome Similarity Implies that Citrus-Parasitic Burrowing Nematodes do not Represent a Unique Species

    OpenAIRE

    Kaplan, D. T.; Opperman, C. H.

    1997-01-01

    Burrowing nematodes from Central America, Dominican Republic, Florida, Guadeloupe, Hawaii, and Puerto Rico were characterized for their ability to parasitize citrus, but citrus parasites were found only in Florida. Sequence tag sites originally amplified from a citrus-parasitic burrowing nematode were polymorphic among 37 burrowing nematode isolates and were not correlated with citrus parasitism, nematode isolate collection site, or amplification of a 2.4-kb sequence tag site (DK#1). Results ...

  4. Appropriate tools of Marketing Information System for Citrus Crop in the Lattakia Region, R. A. SYRIA

    OpenAIRE

    Sulaiman, H.; K. Malec; M. Maitah

    2014-01-01

    Citrus production represents the only livelihood source for many families in Lattakia region. Citrus farmers are not informed about expected prices. This information is crucial to make business decisions. For the farmers is necessary to take into account the spatial and temporal arbitrage of citrus harvest and storage, which may improve citrus farmer’s position in marketing chain and reduce the influence of intermediaries. The aim of the paper is to verify using of SARIMA models as a tool of ...

  5. The use of kaolin to control Ceratitis capitata in organic citrus groves.

    OpenAIRE

    LO VERDE, G; Caleca, V; LO VERDE, 
V

    2011-01-01

    The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera Tephritidae), is the key pest in some organically managed citrus orchards in Sicily. The effectiveness of processed kaolin (Surround WP) for control of C. capitata damage was tested in field trials carried out in 2003-2004 on two early ripening citrus species: satsuma (Citrus unshiu Markow.) and clementine (Citrus clementina Hort. ex Tan.). Although the number of males captured in trimedlure baited traps was high in both yea...

  6. Integrated pest management in citrus orchards in Antalya (1995-1999)

    OpenAIRE

    Özkan, A.; Akteke, Ş.A.; Kaplan, M.; Gürol, M.; Eray, N.; Dalka, Y.; UYSAL, H.; Aytekin, H.; Akyel, E.; Çelik, G.; ARSLAN, M.; Tuncel, H.

    2008-01-01

    This study relating to integrated pest management citrus orchards consisting Central, Kumluca, Finike and Alanya counties of Antalya has been carried out in 8 orchards in 2124 trees during 1995-1999. The citrus mealybug has been found as the main pest and controlled biologically by releasing Leptomastix dactylopii as parasitoid and Cryptolaemus montrouzieri as predator. Citrus whitefly, Citrus red mite and Carob moth have been found as the secondary pests. Mineral oils and specific aca...

  7. Complete Genome sequence of citrus huanglongbing bacterium, ‘Candidatus Liberibacter asiaticus’ obtained through metagenomics

    Science.gov (United States)

    Citrus huanglongbing (HLB) is the most destructive disease of citrus worldwide. It is spread by the citrus psyllids (Diaphorina citri and Trioza erytreae), and is associated with low-titer, phloem-limited infections by any of the three uncultured species of a-Proteobacteria: 'Candidatus Liberibact...

  8. Bacterial brown leaf spot of citrus, a new disease caused by Burkholderia andropogonis

    Science.gov (United States)

    A new bacterial disease of citrus was recently identified in Florida and named as bacterial brown leaf spot (BBLS) of citrus. BBLS-infected citrus displayed flat, circular and brownish lesions with water-soaked margins surrounded by a chlorotic halo on leaves. Based on Biolog carbon source metabolic...

  9. Detection of Citrus Huanglongbing by Fourier Transform Infrared-Attenuated Total Reflection (FTIR-ATR) Spectroscopy

    Science.gov (United States)

    Citrus Huanglongbing (HLB, also known as citrus greening disease) was discovered in Florida in 2005 and is spreading rapidly amongst the citrus growing regions of the state. Detection via visual symptoms of the disease is not a long term viable option. New techniques are being developed to test fo...

  10. Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants

    NARCIS (Netherlands)

    Araujo, W.L.; Marcon, J.; Maccheroni, jr. W.; Elsas, van J.D.; Vuurde, van J.W.L.; Azevedo, de J.L.

    2002-01-01

    Citrus variegated chlorosis (CVC) is caused by Xylella fastidiosa, a phytopathogenic bacterium that can infect all Citrus sinensis cultivars. The endophytic bacterial communities of healthy, resistant, and CVC-affected citrus plants were studied by using cultivation as well as cultivation-independen

  11. 78 FR 58992 - Notice of Request for Extension of Approval of an Information Collection; Citrus Canker...

    Science.gov (United States)

    2013-09-25

    ... States. Citrus canker is a plant disease that affects plant and plant parts, including fresh fruit of... Collection; Citrus Canker; Interstate Movement of Regulated Nursery Stock and Fruit From Quarantined Areas... nursery stock and fruit from quarantined areas to prevent the spread of citrus canker. DATES: We...

  12. 7 CFR 457.107 - Florida citrus fruit crop insurance provisions.

    Science.gov (United States)

    2010-01-01

    ... individual citrus fruit from Citrus IV, V, VII, and VIII to be unmarketable as fresh fruit; or (7) Diseases... 7 Agriculture 6 2010-01-01 2010-01-01 false Florida citrus fruit crop insurance provisions. 457... INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE COMMON CROP INSURANCE REGULATIONS § 457.107 Florida...

  13. 7 CFR 301.75-15 - Funds for the replacement of commercial citrus trees.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Funds for the replacement of commercial citrus trees... trees. Subject to the availability of appropriated funds, the owner of a commercial citrus grove may be eligible to receive funds to replace commercial citrus trees in accordance with the provisions of...

  14. 7 CFR 457.119 - Texas citrus fruit crop insurance provisions.

    Science.gov (United States)

    2010-01-01

    ... fruit caused by low air temperatures. Harvest. The severance of mature citrus fruit from the tree by... of trimming the sides of the citrus trees for better or more fruitful growth of the citrus fruit... you must report by type: (1) The number of trees damaged, topped, hedged, pruned or removed;...

  15. 7 CFR 905.149 - Procedure for permitting growers to ship tree run citrus fruit.

    Science.gov (United States)

    2010-01-01

    ... Fruit § 905.149 Procedure for permitting growers to ship tree run citrus fruit. (a) Tree run citrus fruit. Tree run citrus fruit as referenced in this section is defined in the Florida Department of... grower shall apply to ship tree run fruit using a Grower Tree Run Certificate Application, furnished...

  16. Repellency of Selected Psidium guajava cultivars to the Asian citrus psyllid, Diaphorina citri

    Science.gov (United States)

    Asiatic huanglongbing (HLB)(also known as citrus greening disease) is the most devastating disease of citrus worldwide. It is caused by a bacterium ‘Candidatus Liberibacter asiaticus’ and transmitted by the Asian citrus psyllid (ACP), Diaphorina citri. Considerable research has been conducted toward...

  17. Factors affecting transmission rates of 'Candidatus Liberibacter asiaticus' by Asian citrus psyllid

    Science.gov (United States)

    The Asian citrus psyllid (ACP), Diaphorina citri, is an important pest because it transmits a bacterium ‘Candidatus Liberibacter asiaticus’ (CLas) responsible for a serious disease of citrus known as Asiatic huanglongbing (citrus greening disease). USDA-ARS researchers recently established a program...

  18. 7 CFR 301.75-17 - Funds for the replacement of certified citrus nursery stock.

    Science.gov (United States)

    2010-01-01

    ...) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE DOMESTIC QUARANTINE NOTICES Citrus Canker Notice of Quarantine and Regulations § 301.75-17 Funds for the replacement of certified citrus... 7 Agriculture 5 2010-01-01 2010-01-01 false Funds for the replacement of certified citrus...

  19. Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication

    Science.gov (United States)

    Cultivated citrus are selections from, or hybrids of, wild progenitor species whose identities and contributions to citrus domestication remain controversial. Here we sequence and compare citrus genomes—a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-o...

  20. Cover crop with Teramnus labialis in a citrus orchard: effects on some physical properties of the soil / Cubierta vegetal con Teramnus labialis en plantaciones citrícolas: efectos sobre algunas propiedades físicas del suelo Cubierta vegetal con Teramnus labialis en plantaciones citrícolas: efectos sobre algunas propiedades físicas del suelo

    Directory of Open Access Journals (Sweden)

    Leydis Castellano Rodríguez

    2009-03-01

    Full Text Available The use of leguminous cover crops in citrus orchards constitutes a viable alternative for the improvement of soil properties, whenever they are appropriately managed. In Ciego de Avila University, Cuba, it was evaluated the effect of a leguminous cover crop on some properties of an orchard soil. The work was carried out during four years in an orange plantation of Valencia late (Citrus sinensis L. Osbeck in a 22 years-old orchard, with a plantation frame of 8 X 4 m, planted on a typical red Ferralitic soil, belonging to the CPA ¨José Martí¨, in Ciego de Avila. It was used a random block design with three treatments: one with covering of Teramnus labialis (T1, one with expontaneous vegetation (T2 and the third with no vegetation (T3. The functional structure properties of the soil were determined, and also the composition of macroaggregates expressed in the structure coefficient and the percentage of stable added in water, soil density, humidity and porosity. The increments in the humidity of the soil, the specific volume of pores and air, the structure coefficient, as well as the percentage of stable added in water, in the soil where the covering of Teramnus labialis was stablished, show the efficiency of cover crops in these citrus orchards.El uso de coberturas vivas de leguminosas en plantaciones citrícolas constituye una alternativa viable para el mejoramiento de las propiedades de los suelos, siempre que ellas se manejan adecuadamente en estas áreas. En la universidad de Ciego de Ávila, Cuba se viene trabajando en la evaluación del efecto de coberturas vivas de leguminosas en plantaciones citrícolas en producción, con el objetivo de evaluar el efecto que ejerce la cobertura de leguminosa sobre algunas propiedades del suelo. El trabajo se realizó durante cuatro años (2001-2005 en una plantación de naranja Valencia Late ( Citrus sinensis L. Osbeck en producción de 22 años, con marco de plantación de 8 X 4 m, plantada sobre un

  1. Locais e período de alimentação da cigarrinha vetora de Xylella fastidiosa, Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae, em mudas cítricas Feeding sites and food intake of Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae, a sharpshooter vector of Xylella fastidiosa, on citrus plants

    Directory of Open Access Journals (Sweden)

    Marcelo Pedreira de Miranda

    2008-12-01

    Full Text Available A cigarrinha Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae é um importante vetor da bactéria Xylella fastidiosa, agente causal da clorose variegada dos citros. Este trabalho teve como objetivo identificar o local preferido de alimentação e o período de maior atividade alimentar desta cigarrinha em citros, no sentido de elucidar o comportamento alimentar relacionado à transmissão da bactéria. O local de alimentação foi estudado em ensaio de escolha, no qual 30 insetos adultos foram liberados em gaiolas de observação (n = 10 contendo uma muda de laranja-doce [Citrus sinensis (L. Osbeck]. Após 1; 15; 21; 25; 39; 45 e 49 h da liberação, contaram-se os insetos na parte superior (ramos com brotações e inferior (haste principal, até H"40 cm de altura da muda. Nos ramos da parte superior, avaliou-se a preferência entre a haste, o pecíolo e o limbo foliar. Em um segundo ensaio, 20 machos e 20 fêmeas de B. xanthophis foram confinados individualmente sobre a haste de 'seedlings' de laranja-doce para determinar os períodos de alimentação, quantificando-se a excreção de 'honeydew' (medida indireta da ingestão em períodos sucessivos de dia e noite, durante 48 h. A maioria dos indivíduos de B. xanthophis preferiu a haste dos ramos novos (62%, na parte superior da muda cítrica (91%. Nos 'seedlings', observou-se maior volume de excreção e proporção de indivíduos excretando durante a fotofase, independentemente do sexo. Portanto, em estudos de transmissão de X. fastidiosa, deve-se considerar a preferência de B. xanthophis pela haste de brotações cítricas e sua maior atividade alimentar durante a fotofase.The sharpshooter Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae is an important vector of Xylella fastidiosa, a bacterium that causes citrus variegated chlorosis. This study aimed to identify the preferred feeding sites and periods of this vector on citrus, in order to improve our understanding of the

  2. Nonlinear models for describing the Citrus Variegated Chlorosis in groves of two counties at northwestern Paraná state, Brazil = Modelo não-linear para a curva de progresso de incidência da Clorose Variegada dos Citros (CVC em dois municípios da região noroeste do Estado do Paraná

    Directory of Open Access Journals (Sweden)

    Clara Matiko Ueda

    2010-10-01

    Full Text Available In Brazil, the production of sweet oranges has been threatened by the Citrus Variegated Chlorosis (CVC incited by the gram-negative bacterium Xylella fastidiosa (Wells. Commercial citrus groves in two counties at the Northwestern Paraná state were evaluated to estimate the disease progression by using parameterizations of nonlinear models. Groves of Citrus sinensis Osbeck, variety “Pêra”, “Valência”, “Natal” and “Folha Murcha” had all the plants evaluated for the presence of disease symptoms. Thereafter, different parameterizations of the Logistic and Gompertz models were fitted to these data. The goodness of fit was evaluated by the intrinsic (IN and parameter-effects (PE curvatures of Bates and Watts, the bias of Box and the Hougaard measures of skewness. In Loanda, the best model was the Fermi-Dirac, and in Nova Esperança the data were best fitted to the parameterization L5, which is also a parameterization from the Logistic model.A citricultura é afetada por diversas doenças, dentre as quais a Clorose Variegada dos Citros (CVC causada pela bactéria Xylella fastidiosa (Wells. Para a região noroeste do Estado do Paraná, onde foi avaliada a CVC, propõe-se encontrar modelos não-lineares de curvas de progresso de incidência da CVC que representem o percentual de plantas acometidas pela doença. Para avaliar o comprometimento dos pomares com relação à doença, foram escolhidos pomares comerciais em dois municípios, onde foi determinada a proporção de plantas doentes. Foram selecionados talhões de laranja doce (Citrus sinensis Osbeck nas variedades “Pêra”, “Valência”, “Natal” e “Folha Murcha” e a avaliação de todas as plantas do talhão foi realizada visualmente em relação à presença ou à ausência de sintomas de CVC. Para estimar o modelo que melhor se ajustou aos dados de progresso da proporção da doença em cada talhão, foram considerados modelos não-lineares decrescimento sigmoidal

  3. Avaliação da eficiência da mistura pronta de glyphosate + 2,4-D no controle da Commelina virginica L. em citros Efficacy evaluation of ready mixture of glyphosate + 2,4-D in Commelina virginica L. control in citrus

    Directory of Open Access Journals (Sweden)

    Hamilton H. Ramos

    1996-01-01

    Full Text Available Um dos herbicidas mais utilizados para o controle de plantas daninhas em pós-emergência nos pomares cítricos paulistas é o glyphosate. No entanto, este herbicida aplicado isoladamente e nas doses recomendadas, tem proporcionado seleção da planta daninha trapoeraba (Commelina virginica L., devido à grande tolerância da mesma, somada à eficiente eliminação das demais espécies da comunidade infestante. Em vista disso, e pela falta de opções , faz-se necessária a pesquisa de outros herbicidas ou misturas de herbicidas que sejam eficientes no controle da trapoeraba, de baixa toxicidade para os aplicadores, sistêmicos, e que sejam seletivos às plantas cítricas. O presente trabalho foi desenvolvido com o objetivo de estudar a eficiência da mistura pronta dos herbicidas glyphosate + 2,4-D amina no controle da trapoeraba em citros [Citrus sinensis (L. Osbeck], em comparação com estes mesmos herbicidas aplicados isoladamente, bem como os possíveis efeitos fitotóxicos da mesma à cultura. O experimento foi instalado na região de Catanduva-SP, em um pomar de laranja Pera clone Rio, enxertada sobre limão Cravo, com dez anos de idade, plantado em um espaçamento de 8,0 x 6,5 m. A aplicação dos herbicidas foi realizada em 20 de fevereiro de 1991, de forma dirigida, em pós-emergência tardia da trapoeraba, quando a mesma já florescia e tinha altura variável entre 15 e 60 cm. A análise e interpretação dos resultados obtidos mostraram que a mistura pronta de glyphosate + 2,4-D apresenta um controle superior da trapoeraba em relação aos produtos aplicados isoladamente, não havendo diferenças significativas no controle para doses superiores a 0,60 + 0,80 kg i.a./ha. Os dados obtidos reforçam a teoria de sinergismo entre os dois produtos. Em nenhum dos tratamentos foi verificado sintomas visuais de intoxicação nas laranjeiras.In citrus orchard of São Paulo state, Brazil, one of the most employed post-emergency herbicide for

  4. 75 FR 28233 - Availability of an Environmental Assessment for a Biological Control Agent for Asian Citrus Psyllid

    Science.gov (United States)

    2010-05-20

    .... ] ACP is of particular concern since it is a carrier of Huanglongbing disease (citrus greening), which is considered to be one of the most serious citrus diseases in the world. Citrus greening is a... Control Agent for Asian Citrus Psyllid AGENCY: Animal and Plant Health Inspection Service, USDA....

  5. 75 FR 34419 - Notice of Revision and Request for Extension of Approval of an Information Collection; Citrus...

    Science.gov (United States)

    2010-06-17

    ... canker is a plant disease that affects plant and plant parts, including fresh fruit of citrus and citrus... Information Collection; Citrus Canker; Interstate Movement of Regulated Nursery Stock and Fruit from... nursery stock and fruit from quarantined areas to prevent the spread of citrus canker and to request...

  6. Study of the thermal degradation of citrus seeds

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez-Montoya, V. [Centro de Quimica, Instituto de Ciencias, Universidad Autonoma de Puebla, Apdo. Postal J-55, Puebla 72570, Pue (Mexico); Instituto Nacional del Carbon, CSIC, Apartado 73, E-33080 Oviedo (Spain); Montes-Moran, M.A. [Instituto Nacional del Carbon, CSIC, Apartado 73, E-33080 Oviedo (Spain); Elizalde-Gonzalez, M.P. [Centro de Quimica, Instituto de Ciencias, Universidad Autonoma de Puebla, Apdo. Postal J-55, Puebla 72570, Pue (Mexico)

    2009-09-15

    The citrus seeds are one of the principal residues in the juice industry and their utilization can decrease significantly the problems of their final disposal. In this work the thermal degradation of three Mexican citrus seeds: orange (Citrus sinensis), lemon (Citrus Limon) and grapefruit (Citrus paradisi) was studied in nitrogen atmosphere. The two components (embryo and husk) of the seeds were characterized separately. The results showed that the thermal effects are very similar between the three embryos and the three husks. The embryos show higher degradability, superior content of nitrogen and higher heating value than the husks. The thermal degradation of the components of the three seeds is completed at 600 C and it is considered to be a global process derived from the decomposition of their principal components (cellulose, hemicellulose and lignin). The results suggest that mixing the three entire seeds will not lead to a severe deviation from their individual thermal behavior and that the industry could apply them for carbonization purposes. (author)

  7. The Role of Image Enhancement in Citrus Canker Disease Detection

    Directory of Open Access Journals (Sweden)

    K. Padmavathi

    2016-09-01

    Full Text Available Digital image processing is employed in numerous areas of biology to identify and analyse problems. This approach aims to use image processing techniques for citrus canker disease detection through leaf inspection. Citrus canker is a severe bacterium-based citrus plant disease. The symptoms of citrus canker disease typically occur in the leaves, branches, fruits and thorns. The leaf images show the health status of the plant and facilitate the observation and detection of the disease level at an early stage. The leaf image analysis is an essential step for the detection of numerous plant diseases. The proposed approach consists of two stages to improve the clarity and quality of leaf images. The primary stage uses Recursively Separated Weighted Histogram Equalization (RSWHE, which improves the contrast level. The second stage removes the unwanted noise using a Median filter. This proposed approach uses these methods to improve the clarity of the images and implements these methods in lemon citrus canker disease detection.

  8. Identification of photoperception and light signal transduction pathways in citrus

    Directory of Open Access Journals (Sweden)

    Vera Quecini

    2007-01-01

    Full Text Available Studies employing model species have elucidated several aspects of photoperception and light signal transduction that control plant development. However, the information available for economically important crops is scarce. Citrus genome databases of expressed sequence tags (EST were investigated in order to identify genes coding for functionally characterized proteins responsible for light-regulated developmental control in model plants. Approximately 176,200 EST sequences from 53 libraries were queried and all bona fide and putative photoreceptor gene families were found in citrus species. We have identified 53 orthologs for several families of transcriptional regulators and cytoplasmic proteins mediating photoreceptor-induced responses although some important Arabidopsis phytochrome- and cryptochrome-signaling components are absent from citrus sequence databases. The main gene families responsible for phototropin-mediated signal transduction were present in citrus transcriptome, including general regulatory factors (14-3-3 proteins, scaffolding elements and auxin-responsive transcription factors and transporters. A working model of light perception, signal transduction and response-eliciting in citrus is proposed based on the identified key components. These results demonstrate the power of comparative genomics between model systems and economically important crop species to elucidate several aspects of plant physiology and metabolism.

  9. [Chemical composition and bioactive compounds of flour of orange (Citrus sinensis), tangerine (Citrus reticulata) and grapefruit (Citrus paradisi) peels cultivated in Venezuela].

    Science.gov (United States)

    Rincón, Alicia M; Vásquez, A Marina; Padilla, Fanny C

    2005-09-01

    The purpose of this research was to evaluate the chemical composition and some bioactive compounds in the peel's flour of some of the most consumed citrus fruits cultivated in Venezuela. Chemical composition as well as some trace elements, ascorbic acid, carotenoids dietary fiber, total polyphenols and their antiradical efficiency, using the 2,2-diphenyl-1-picrylhidracyl (DPPH) were assessed in the dried peels of orange (Citrus sinensis), tangerine (Citrus reticulata) and white grapefruit (Citrus paradisi). Moisture, fat, protein and ash content for all samples showed statistical differences (p peel showed the highest magnesium and carotenoid content, while highest ascorbic acid and carotenoid content was found in the grapefruit's peel. Dietary fiber content presented significant high value in the tangerine peel. All samples presented high content of extractable polyphenols (4.33; 7.6 and 5.1 g/100g). The highest antiradical efficiency was shown by the tangerine's peel, value which correlates with the polyphenol content. These results suggest that tangerine peel should be the most suitable, to reduce risk of some diseases such as cardiovascular and some associated to lipid oxidation. Studied samples are good sources of dietary fiber and phenolic compounds, whose use could be useful in the formulation of functional foods, taking advantage of the presence of dietary fiber and antioxidant compounds in only one ingredient. PMID:16454058

  10. 不同pH值下接种AM真菌对枳橙苗生长及光合作用的影响%Effects of AM Fungi on Growth and Photosynthesis of Citrus Rootstock Seedlings in Soils with Different pH

    Institute of Scientific and Technical Information of China (English)

    牛琳琳; 高崇; 曾明; 李伟; 雷世梅; 伍加勇

    2012-01-01

    以枳橙Citrus sinensis×Poncirus tri foliata为试材,研究不同pH值(5.5~6.5,6.5~7.5,7.5~8.5)条件下接种丛枝菌根(AM)真菌摩西球囊霉Glomus mosseae对枳橙苗生长及光合能力的影响.结果表明,不同pH值水平下接种摩西球囊霉均能有效地侵染枳橙植株,菌根侵染率在65%以上;接种处理植株的菌根侵染率、株高、生物量干重、叶片叶绿素含量、光合作用速率和蒸腾速率等指标均显著高于不接种植株;接种AM真菌增强了宿主植物对碱性土的适应性.%Citrange seedlings (Citrus sinensis (L. ) Osbeck×Poncirus trifoliata (L. )) were used to study the effect of arbuscular mycorrhizal (AM) fungus (Glomus mossease) on growth and photo-. synthesis of the trees under different pH conditions (pH 5. 5~6. 5, 6. 5~7. 5, 7. 5~8. 5). Results indicated that the mycorrhizal fungus Glomus mossease was equally effective in infecting plants under all tested pH conditions and the infection rates were higher than 65%. Mycorrhiza infection rate, plant height, dry weight, chlorophyll content, photosynthetic activity and transpiration rate of inoculated plants were significantly higher than those of control plants. Inoculation with AM fungi enhanced the adaptability of the host plants to alkaline soils.

  11. Construction of Plant Expression Vectors with PMI Gene as Selection Marker and Their Utilization in Transformation of Citrus sinensis%PMI基因作为选择标记的植物表达载体构建及其在雪柑转基因中的应用

    Institute of Scientific and Technical Information of China (English)

    曾黎辉; 徐海峰; 王会全; 吴少华; 朱艺萱

    2008-01-01

    以大肠杆菌(Escherichia coli)6-磷酸甘露糖异构酶(6-phosphomannose isomerase,PMI)基因替换植物表达载体pCAMBIA1301中的hpt基因以及pBI121中的gus基因,构建了以PMI基因为选择标记基因的植物表达载体pCAMBIA1301PMI和pBIPMI,并导人根癌农杆菌(Agrobacterium tumefaciens)EHA105中.研究了两种表达载体对雪柑(Citrus sinensis L.Osbeck)上胚轴的转化,在培养基附加25 g/L甘露糖和5 g/L蔗糖为碳源的选择压力下,pCAMBIA1301PMI的转化率为27.7%,pBIPMI转化率为12.7%,对再生植株用氯酚红和PCR检测证实了PMI基因的导入,建立了以PMI/甘露糖为选择系统的雪柑转基因体系.

  12. Observations on Morphological Abnormalities of the Vessel Elements of Veins and Fruit of Citrus under Boron Deficiency%缺硼导致柑橘叶脉和果实导管分子形态畸形的观察

    Institute of Scientific and Technical Information of China (English)

    李爽; 彭抒昂; 刘永忠; 周高峰; 杨成泉

    2012-01-01

    缺硼可导致柑橘叶片变厚而脆、发生木栓化、“猴头果”等现象,其原因是植物组织内部起运输作用的维管组织受到损坏.导管是维管组织重要组成部分,本实验以‘纽荷尔’脐橙(Citrus sinensis Osbeck‘ Newhall')和HB柚[Citrus maxima(Burm.)Merr.‘HB You’]叶片、HB柚果实中果皮为材料,利用离析方法将导管分离,观察缺硼导管分子形态的变化.结果表明:(1)缺硼使纽荷尔脐橙叶脉中的孔纹导管数量增多;(2)缺硼使HB柚叶片和果实中果皮的孔纹导管大量增生,梯纹导管和网纹导管数量减少;(3)正常导管圆润饱满,而缺硼导管变形干瘪,且有侧壁穿孔现象;(4)缺硼导管变得短而窄,细胞壁变薄,无尾率增加,使维管组织功能丧失.(5)缺硼维管束受损,运输效率降低,是发生叶脉木栓化和“猴头果”现象的源头.%Boron deficiency resulted in thick and brittle leaves, suberification, and "contract fruit" in citrus, due to damaged vascular tissue, which usually functions for transportation. Vessels are an important component of vascular tissue. Morphological abnormalities of the vessel elements were observed using the leaves of ' Newhall' (Citrus sinensis Osbeck) Navel orange and HB Pummelo [ Citrus maxima ( Burm. ) Merr. ] by separating the vessels by means of segregation. Results indicated that; (1) The number of pitted vessels in 'Newhall' Navel oranges with boron deficiency increased; (2) A larger number of pitted vessels in the nervure and mesocarp of HB Pummelo with boron deficiency were proliferated, while scalari-form vessels and reticulate vessels decreased; (3) Normal vessels were round and full, while vessels under boron deficiency were transubstantiated shriveled; (4) Compared with the control vessels, vessels under treatment became short and narrow, cell walls turned thin and the percentage of no-tail under boron deficiency condition was increased, which finally led the vascular tissue to

  13. Induced resistance against the Asian citrus psyllid, Diaphorina citri, by β-aminobutyric acid in citrus.

    Science.gov (United States)

    Tiwari, Siddharth; Meyer, Wendy L; Stelinski, Lukasz L

    2013-10-01

    β-Aminobutyric acid (BABA) is known to induce resistance to microbial pathogens, nematodes and insects in several host plant/pest systems. The present study was undertaken to determine whether a similar effect of BABA occurred against the Asian citrus psyllid, Diaphorina citri Kuwayama, in citrus. A 25 mM drench application of BABA significantly reduced the number of eggs/plant as compared with a water control, whereas 200 and 100 mM applications of BABA reduced the numbers of nymphs/plant and adults/plants, respectively. A 5 mM foliar application of BABA significantly reduced the number of adults but not eggs or nymphs when compared with a water control treatment. In addition, leaf-dip bioassays using various concentrations (25–500 mM) of BABA indicated no direct toxic effect on 2nd and 5th instar nymphs or adult D. citri. BABA-treated plants were characterized by significantly lower levels of iron, magnesium, phosphorus, sodium, sulfur and zinc as compared with control plants. The expression level of the PR-2 gene (β-1,3-glucanase) in BABA-treated plants that were also damaged by D. citri adult feeding was significantly higher than in plants exposed to BABA, D. citri feeding alone or control plants. Our results indicate the potential for using BABA as a systemic acquired resistance management tool for D. citri.

  14. The push-pull strategy for citrus psyllid control.

    Science.gov (United States)

    Yan, Huaxue; Zeng, Jiwu; Zhong, Guangyan

    2015-07-01

    The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the only natural vector of Candidatus Liberibacter asiaticus that causes citrus huanglongbing (HLB), a most destructive disease of citrus. Currently, no remedial therapy exists for the disease, and so effective control of ACP is very important in curbing the transmission of the disease. The push-pull strategy should be thoroughly explored as an approach to ACP management. This mini-review summarises the current progress towards more effective repellent and attractant chemicals through investigating known repellent and attractive plants. Interactions between ACP and its host plants are also addressed, with emphasis on the possible involvement of the host biochemicals in attracting the insect. Potential ways to increase the effectiveness of the pull-push strategy are briefly discussed. It is expected that the pull-push strategy will be gradually developed following more extensive research. PMID:25256398

  15. Natural bioactive compounds of Citrus limon for food and health.

    Science.gov (United States)

    González-Molina, E; Domínguez-Perles, R; Moreno, D A; García-Viguera, C

    2010-01-20

    Citrus genus is the most important fruit tree crop in the world and lemon is the third most important Citrus species. Several studies highlighted lemon as an important health-promoting fruit rich in phenolic compounds as well as vitamins, minerals, dietary fiber, essential oils and carotenoids. Lemon fruit has a strong commercial value for the fresh products market and food industry. Moreover, lemon productive networks generate high amounts of wastes and by-products that constitute an important source of bioactive compounds with potential for animal feed, manufactured foods, and health care. This review focuses on the phytochemistry and the analytical aspects of lemon compounds as well as on the importance for food industry and the relevance of Citrus limon for nutrition and health, bringing an overview of what is published on the bioactive compounds of this fruit. PMID:19748198

  16. The push-pull strategy for citrus psyllid control.

    Science.gov (United States)

    Yan, Huaxue; Zeng, Jiwu; Zhong, Guangyan

    2015-07-01

    The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the only natural vector of Candidatus Liberibacter asiaticus that causes citrus huanglongbing (HLB), a most destructive disease of citrus. Currently, no remedial therapy exists for the disease, and so effective control of ACP is very important in curbing the transmission of the disease. The push-pull strategy should be thoroughly explored as an approach to ACP management. This mini-review summarises the current progress towards more effective repellent and attractant chemicals through investigating known repellent and attractive plants. Interactions between ACP and its host plants are also addressed, with emphasis on the possible involvement of the host biochemicals in attracting the insect. Potential ways to increase the effectiveness of the pull-push strategy are briefly discussed. It is expected that the pull-push strategy will be gradually developed following more extensive research.

  17. The IDA peptide controls abscission in Arabidopsis and Citrus

    Directory of Open Access Journals (Sweden)

    Leandro H Estornell

    2015-11-01

    Full Text Available Organ abscission is an important process in plant development and reproduction. During abscission, changes in cellular adhesion of specialised abscission zone (AZ cells ensure the detachment of infected organs or those no longer serving a function to the plant. In addition, abscission also plays an important role in the release of ripe fruits. Different plant species display distinct patterns and timing of organ shedding, most likely adapted during evolution to their diverse life styles. However, it appears that key regulators of cell separation may have conserved function in different plant species. Here we investigate the functional conservation of the citrus orthologue of the Arabidopsis peptide ligand INFLORESCENCE DEFICIENT IN ABSCISSION (AtIDA, controlling floral organ abscission. We discuss the possible implications of modifying the citrus IDA orthologue for citrus fruit production.

  18. Analysis of SSR in Citrus Sequences from EMBL Database

    Institute of Scientific and Technical Information of China (English)

    MENG Hai-jun; CAO Qing-qin; HU Zhi-yong; LIU Gao-ping; CHENG Yun-jiang; DENG Xiu-xin

    2005-01-01

    Abundance of simple sequence repeat (SSR) in Citrus sequences from EMBL database was investigated by using computer program MISA (MIcroSAtellite), which aimed to provide useful information for the development of SSR markers.Among 32 896 sequences of Citrus, 4987 SSRs were found in 4167 sequences and the average distance between SSRs was approximately 3.5 kb. Mononucleotide repeats (50.6%) were the most abundant repeats. And di-, tri-, tetra-, penta- and hexa-nucleotide repeats were 22.8, 25.2, 1, 0.08, and 0.36%, respectively. The most abundant motif was A/T followed in descending order by AG/CT, AC/GT, AT/TA. AAT/ATT, AAG/CTT, AGC/CGT, ACG/CTG and C/G. They comprised about90% of all microsatellites. Ten primer pairs were designed, and three of them produced clear visible bands among Citrus and its related genera.

  19. In Vitro Propagation of Citrus Rootstocks

    Directory of Open Access Journals (Sweden)

    Suneel SHARMA

    2009-06-01

    Full Text Available Present investigation was conducted to standardize a protocol for in-vitro propagation of citrus rootstocks viz. Rough lemon, Cleopatra mandarin Pectinifera and Troyer citrange. The shoot tip explant was found better for callus induction of these rootstocks than the nodal segment. Maximum callus formation (40.0% and 23.3% of shoot tip explants was obtained in Cleopatra mandarin, Pectinifera, and Rough lemon and Troyer citrange, respectively in treatment MS basal media + 0.5mg/l Kin, 2.0mg/l NAA, and 2.0mg/l 2, 4-D. Furthermore, the maximum number of shoots per explant was obtained through the callus in Pectinifera, Rough lemon and Cleopatra mandarin in MS basal media + BAP 1mg/l. Maximum rooting of shoots (1.11% was noted in rootstock Rough lemon followed by Cleopatra mandarin for the � MS media supplemented with 10mg/l IBA. Although the callus development and bud proliferation was recorded in rootstock Troyer citrange however, shoot and root formation did not occur. The potting media consisting of soil, sand and FYM in the ratio of 1:1:1 by volume was better with maximum survival rate of hardened plants six weeks after transferring to the pots under greenhouse for Rough lemon followed by Pectinifera and Cleopatra mandarin rootstock.

  20. Technological quality of irradiated Moroccan citrus fruits

    International Nuclear Information System (INIS)

    The effect of irradiation at doses of 125, 250, 375, and 500 Gy, commonly used for quarantine treatment, on the quality of Maroc-late orange, the most common export variety of Morocco was investigated. In the first study fruits were irradiated without any previous cold conditioning treatment as practiced by the export trade for quarantine purposes. In the second study fruits obtained from the normal chain after conditioning was irradiated. Storage of irradiated fruits was studied at room temperature and 10 deg. C at 0 deg. C in case of control fruits. The parameters studied included juice yield, total solids, reducing and total sugars, total acids and volatile acids, dry weight and weight loss. The results showed that irradiation did not affect the technological quality of citrus fruits during four weeks storage. The result thus far points to the possibility for the successful application of irradiation as an alternative quarantine treatment to the classical methods, which result in browning of the peel. The browning phenomenon could be controlled by waxing and will be the subject of a future study. (author)

  1. Performance of 'Okitsu' Satsuma Mandarin on nine rootstocks

    OpenAIRE

    Zuleide Hissano Tazima; Carmen Silvia Vieira Janeiro Neves; Inês Fumiko Ubukata Yada; Rui Pereira Leite Júnior

    2013-01-01

    Mandarins have become increasingly valued as citrus fruits for the fresh market due to the easy peeling, attractive flavor, and health and nutritional properties. Plant growth and yield, and characteristics of fruits of 'Okitsu' Satsuma mandarin (Citrus unshiu Marc.) trees grafted on nine rootstocks were evaluated in Londrina, northern Paraná, Brazil. The rootstocks were: 'Rangpur' lime (Citrus limonia Osb.); 'Cleopatra' (Citrus reshni hort. ex Tanaka) and 'Sunki' mandarins (Citrus sunki hort...

  2. Distribuição espacial de Toxoptera citricida (Kirkaldy (Hemiptera: Aphididae na cultura de citros Spatial distribution of Toxoptera citricida (Kirkaldy (Hemiptera: Aphididae in citrus orchards

    Directory of Open Access Journals (Sweden)

    Francisco Ricardo de Toledo

    2006-08-01

    Full Text Available O estudo da distribuição espacial de pragas é fundamental para elaboração de planos de amostragem para o uso do manejo integrado de pragas. Para o afídeo Toxoptera citricida (Kirkaldy, estudou-se a distribuição espacial em talhões de pomares de citros comerciais de laranja-doce [Citrus sinensis (L. Osbeck] da variedade Pêra, com 5; 9 e 15 anos de idade, durante o período de setembro de 2004 a abril de 2005. Foram realizadas 14 amostragens de número de T. citricida em intervalos aproximados de 15 dias entre as mesmas, utilizando-se de armadilhas adesivas de cor amarela (0,11 x 0,11 m fixadas à planta, a 1,5 m de altura aproximadamente. As armadilhas foram distribuídas na área, a cada cinco plantas na linha, em linhas alternadas, totalizando 137 armadilhas no talhão com 5 anos, 140 no talhão com 9 anos e 80 no talhão com 15 anos. Os índices de dispersão utilizados foram: razão variância média (I, índice de Morisita (Idelta, coeficiente de Green (Cx e expoente k da distribuição Binomial Negativa. O índice que melhor representou a agregação do pulgão foi o expoente k da distribuição Binomial Negativa, e a distribuição binomial negativa foi o modelo que melhor se ajustou aos dados. Através destas análises, verificou-se que a maioria das amostragens apresentou uma distribuição agregada da população de T. citricida.The study of insects' spatial distribution is fundamental to elaborate potential sampling plans to be used in integrated pest management. Spatial distribution of Toxoptera citricida was studied in plots of commercial orange orchards (Citrus sinensis from 'Pêra' variety of 5, 9 and 15 years old, respectively, from September 2004 to April 2005. It was carried through 14 fortnightlies Toxoptera citricida samplings numbers, utilizing yellow adhesive traps (0.11 x 0.11 m settled on the plant at 1.5 m of height approximately. The traps were distributed in the area each five plants in the line, in alternated

  3. Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence

    OpenAIRE

    Navarro Luis; Ollitrault Patrick; Pina José A; Hernández María; Juárez José; Aleza Pablo

    2009-01-01

    Abstract Background In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus species are characterized by high heterozygosity, making it difficult to assemble large genome sequences. Thus, the International Citrus Genomic Consortium (ICGC) decided to establish a reference whole citrus genome sequence from a h...

  4. Targeting juvenile hormone metabolic genes in the Asian citrus psyllid (Diaphorina citri Kuwayama) as a strategy to reduce the spread of citrus greening disease

    OpenAIRE

    Van Ekert, Evelien; Borovsky, D.; Powell, C A; Cave, R. D.; Alessandro, R. T.; Shatters, Jr., R. G.

    2014-01-01

    Diaphorina citri Kuwayama, the Asian citrus psyllid (ACP), is a devastating citrus pest due to its transmission of a phloem-limited bacterial pathogen, Candidatus Liberibacter asiaticus, that causes citrus greening. Psyllid control is a major part of effective greening disease management, and our research targets perturbation of insect juvenile hormone metabolism as a new psyllid control strategy.  Previous studies have shown that application of a juvenile hormone (JH) analogue, pyriproxyfen,...

  5. Chemical compounds effective against the citrus Huanglongbing bacterium 'Candidatus Liberibacter asiaticus' in planta.

    Science.gov (United States)

    Zhang, Muqing; Powell, Charles A; Zhou, Lijuan; He, Zhenli; Stover, Ed; Duan, Yongping

    2011-09-01

    Citrus Huanglongbing (HLB) is one of the most destructive diseases of citrus worldwide and is threatening the survival of the Floridian citrus industry. Currently, there is no established cure for this century-old and emerging disease. As a possible control strategy for citrus HLB, therapeutic compounds were screened using a propagation test system with 'Candidatus Liberibacter asiaticus'-infected periwinkle and citrus plants. The results demonstrated that the combination of penicillin and streptomycin (PS) was effective in eliminating or suppressing the 'Ca. L. asiaticus' bacterium and provided a therapeutically effective level of control for a much longer period of time than when administering either antibiotic separately. When treated with the PS, 'Ca. L. asiaticus'-infected periwinkle cuttings achieved 70% of regeneration rates versus citrus plants. This may provide a useful tool for the management of citrus HLB and other Liberibacter-associated diseases.

  6. Hydrolysates of citrus plants stimulate melanogenesis protecting against UV-induced dermal damage.

    Science.gov (United States)

    Chiang, Hsiu-Mei; Lin, Jen-Wen; Hsiao, Pei-Ling; Tsai, Shang-Yuan; Wen, Kuo-Ching

    2011-04-01

    The sun-tanning process occurs as a spontaneous response to ultraviolet (UV) irradiation. UV will induce tanning and DNA damage, processes that can lead to photoaging and skin disorders such as hyperpigmentation and cancer. The pigment melanin protects skin from UV damage; therefore, an efficient melanin-promoting suntan lotion could be highly beneficial. In this study, a process was developed to increase the content of naringenin in citrus extracts and to determine whether a higher naringenin content of citrus would induce melanogenesis. Melanin content and tyrosinase expression in mouse B16 melanoma cells were assayed after treatment with citrus plant extracts and their hydrolysates. The results indicate that hydrolysis increased the naringenin content in citrus extracts and that citrus preparations stimulated cellular melanogenesis and tyrosinase expression. It is suggested that this method is applicable to the industrial production of melanin-promoting suntan lotions with antiphotocarcinogenic properties derived from citrus rind and citrus products. PMID:20857432

  7. Organic Acid Concentrations and the Relative Enzymatic Changes During the Development of the Citrus Fruits

    Institute of Scientific and Technical Information of China (English)

    LUO An-cai; YANG Xiao-hong; DENG Ying-yi; LI Chun-fan; XIANG Ke-shu; LI Dao-gao

    2003-01-01

    Seasonal changes in enzyme activities and citrate concentration during the development of citrus fruits were investigated. The result showed that the organic acid concentrations reached a peak in the 100 - 130days after anthesis and gradually declined during later stages of fruit maturation in most varieties of citrus,but declined only slightly thereafter in lemon [Citrus lin on (L.) Burm]; there is no relation between the activity of citrate synthetase(CS) and the different acid concentration in different citrus fruits; the increase of the activity of the cytosolic aconitase in the late period of the development of citrus fruits accelerated the degradation of citric acid in citrus fruits; the higher the activity of phosphoenolpyruvate carboxylase(PEPC) and the ratio of NAD-dependent isocitrate hydrogenase(PEPC/NAD-IDH- ), the more the concentrations of organic acids in citrus fruit.

  8. Learning to fight a fly : developing citrus IPM in Bhutan

    OpenAIRE

    Schoubroeck, van, H.J.

    1999-01-01

    The chinese citrus fly is one of the key pests in Bhutanese mandarin orchards that lays eggs in developing fruit that cause pre-mature fruit drop. In this study it is used as a "model subject" to explore the integration of technical, social and administrative domains of knowledge. The confinement of the study to control of the fly leads to the study addressing a broad set of issues that are linked through their relevance to control the pest. Citrus fly control and its consequent doubling of t...

  9. Induced mutations in citrus shoot tip culture in vitro

    International Nuclear Information System (INIS)

    This study was undertaken to determine the efficacy of using induced mutation of the callus of citrus clones as a method in citrus breeding. It was observed that the nuclear clones had a higher ratio of callus cell differentiation than the old clones. The callus cells were exposed to gamma irradiation of 7,000 roentgen at 127 R/min. Callus cells derived from the shoot tip proved to be more appropriate for cytological examination by the smearing technique than the root tip cells. Gamma irradiation of callus cells is quite effective for inducing chromosome aberration and micronucleus inspection

  10. Inhibitory effect of Citrus unshu peel on anaerobic digestion

    Energy Technology Data Exchange (ETDEWEB)

    Mizuki, E.; Akao, T.; Saruwatari, T. (Fukushima Industrial Research Int. of Fukuoka Prefecture (JP))

    1990-01-01

    A significant inhibition of methane fermentation was observed in anaerobic digestion of Citrus unshu peel at loadings above 2g/litre per day. This inhibitory effect was mainly due to peel oil, but in part to other substances present in the peel. The limiting load of peel oil to anaerobic digestion was about 65 {mu}l/litre per day. Addition of peel oil below this limit load resulted in a change in the microbial flora and in gas production greater than that of digestion with no peel oil. Citrus unshu peel did not inhibit methane fermentation after removal of peel oil by steam distillation or aeration. (author).

  11. Energy conservation in citrus processing. Final project report

    Energy Technology Data Exchange (ETDEWEB)

    Leo, M.A.; Lari, R.I.; Moore, N.R.; Broussard, M.R.; Gyamfi, M.

    1981-11-01

    Alternative energy conserving systems for use in citrus processing plants were synthesized and evaluated in terms of energy savings and economic return. The energy intensive operations that are carried out in citrus processing plants include conveying and extraction, concentration, peel drying, refrigeration, and pasteurization. The alternative energy conserving systems are synthesized from components and subsystems that are arranged to make use of energy cascading and thermodynamic regeneration to reduce the overall energy usage. System requirements such as air pollution rules and plant processing load cycles, a characterization of major operations, description of alternative system concepts, and the evaluation of alternative systems in terms of economic parameters and energy usage are identified.

  12. Weed Biomass and Weed Species Diversity of Juvenile Citrus Trees Intercrop with some Arable Crops

    Directory of Open Access Journals (Sweden)

    Patience Mojibade OLORUNMAIYE

    2012-02-01

    Full Text Available A preliminary study was carried out to evaluate the performances of eight crops in the intercrop of citrus with arable crops at the National Horticultural Research Institute (NIHORT Ibadan, Nigeria. Eight arable crops: maize, cucumber, sweet potato, Corchorus olitorius, large green, grain amaranth, Mucuna pruriens var. utilis, and groundnut were intercropped with young citrus trees in the early planting season of 2010 with sole citrus as control. The experiment was laid out in a completely randomized block design with three replicates. Data were collected on weed flora, weed density and weed dry weight. Results showed that the relative frequencies of weeds in all the plots were less than 4% at both 6 and 9WAP. Gomphrena celosoides, Oldenlandia corymbosa and Tridax procumbens were most preponderant in appearing in all the plots. Tridax procumbens had a consistent relative frequency (2.34% in all the plots except in citrus/maize plot (0.78% at 9 WAP. Significantly lower broadleaf weed densities were obtained in citrus/sweet potato, citrus/large green, control plot and citrus/cucumber (28.67, 45.00, 50.00 and 76.33 m-2 respectively than in citrus/groundnut plot (143.00 m-2. Similarly, significantly lower grass weed densities were produced in citrus/Mucuna and citrus/sweet potato (0.33 m-2 each plots than the control plot (11.33 m-2. Whereas citrus/corchorus plot produced significantly lower broadleaf weed dry weight (37.59 g m-2 than citrus/Mucuna plot (126.47 g m-2 at 3WAP, citrus/large green plot (16.15 g m-2 and citrus/groundnut plot (123.25 g m-2 followed the same trend at 6 WAP. Sedges dry weights were less than 7 g m-2 in all the plots compared with control plot.

  13. In vivo Induction of Tetraploid in Tangerine Citrus Plants (Citrus reticulata Blanco) with the Use of Colchicine.

    Science.gov (United States)

    Surson, Suntaree; Sitthaphanit, Suphasit; Wongma, Nattapong

    2015-01-01

    This in vivo experiment was carried out at Sakhon Nakhon Rajabhat University, Sakhon Nakhon Province, Thailand during March-October 2013. The study aims to search for some possibilities in inducing a large number of tetraploid sets of chromosomes in tangerine citrus seedlings with the use of colchicine chemical. A Randomized Complete Block Design (RCBD) with four replications was used. Seeds of tangerine citrus were treated with colchicine solutions. The experiment consisted of seven treatments, i.e., T1 with 0.0% colchicine (control), T2 with 0.2% colchicine solution and submerged for 12 h, T3 with 0.2% colchicine solution and submerged for 24 h, T4 with 0.4% colchicine solution and submerged for 12 h, T5 with 0.4% colchicine solution and submerged for 24 h, T6 with 0.8% colchicine solution and submerged for 12 h, T7 with 0.8% colchicine solution and submerged for 24 h. The experiment was conducted for 91 days. The results showed that colchicine compound severely and significantly affected germination of tangerine citrus seeds. Colchicine of 0.2% in the solution with seeds submerged for 24 h gave the highest percentages of tetraploid chromosomes in seedlings of tangerine citrus (63.64%). Colchicine significantly affected seed germination, plant height, stomata density and leaf index of the tangerine citrus seeds and seedlings. Colchicine had no significant effect on poly-embryos, mono-embryos, leaf number, leaf area, leaf weight, leaf length and stomata length of the tangerine citrus seedlings. PMID:26353415

  14. EFECTO DE LA ÉPOCA DE RECOLECCIÓN DE LA NARANJA (Citrus sinensis L. VARIEDAD VALENCIA 'LATE' SOBRE EL PROCESAMIENTO INDUSTRIAL DE CÍTRICOS EN LAS TRES GRANDES REGIONES EDAFOCLIMÁTICAS DE CUBA

    Directory of Open Access Journals (Sweden)

    Yenia Pérez

    2004-01-01

    Full Text Available El presente estudio se realizó con la especie Citrus sinensis (L Osbeck variedad Valencia 'Late' procedente de 10 empresas citrícolas del país, ubicadas en tres regiones edafoclimáticas, que suministran la fruta a la producción de jugo concentrado congelado de naranja (JCCN, en las plantas industriales de Pinar del Río, Ceballos y Contramaestre durante el período 2000-2002. Se evaluó el comportamiento del rendimiento potencial (RP de la fruta utilizada como materia prima para el procesamiento industrial, obteniendo los menores valores dados por las empresas de la región oriental asociada a laplanta industrial de Contramaestre, que se traduce en destinar menos volúmenes de fruta para producir 1 t de JCCN. Se mantuvo la tendencia de esta planta industrial de obtener la mejor eficiencia del sistema en términos de RP comparadas con las dos restantes en estudio. Estos resultados mostraron que en la región oriental se alcanzaron los mayores valores de SST del sistema, ya que el 91.6 % de la fruta alcanza valores medio desde 11.3 hasta 15.57 0Bx, mientras el 100 % de la fruta alcanza un contenido de jugo por encima de 51.6 %. Las variables de calidad de la fruta asociadas al rendimiento industrial de sólidos solubles totales (SST y contenido de jugo (CJ mostraron un incremento sostenido en febrero-marzo, causando un efecto importante en la fecha de recolección de la naranja Valencia 'Late' durante los tres años evaluados.

  15. Determination of elements in citrus leaves

    International Nuclear Information System (INIS)

    In many agricultural stations and farms most of the problems encountered generally reduce to questions of diminished yield. This may be due to a number of factors, including soil exhaustion and the application of fertilizers of unsuitable formula. The chemical impoverishment of the soil is due to the leaching-out phenomenon, i- e. the washing out of bases, and to the ''exportation'' of fertilizer elements in the form of crops in years when nothing has been returned to the soil. These losses have a particularly adverse effect if the parent rock does not contain sufficient mineral reserves to compensate for them by a slow alteration process. Such impoverishment is revealed by soil and foliar analyses. The authors have attempted to determine the content in citrus plants of the following elements: Mn, P, Cu, Cl and K (the latter on three samples only). After collection, the samples are treated by Bransolten's method (Rapport de Recherche TRICO № 15/1968), dried for at least 12 hours at 105°C, followed by pulverization of the leaves, after which the determination is carried out. The determination of Mn and Cl is very simple, as is that of Cu. The latter is determined by activation with slow neutrons in order to avoid Zn formation. The phosphorus content is determined by measuring the beta-radiation emitted by the radioactive elements. In this case particular precautions must be taken to ensure that the same layer is used for the samples and the standards, since beta-radiation is absorbed by these layers. For the K and Na determinations thermal neutrons are used for activation and a Ge(Li) detector for measurement of the gamma-spectra. Because of the high resolution of the detector, the two elements can be determined without separation. (author)

  16. In vitro mutant obtainment by irradiation of nucellar tissue of citrus (Citrus Sinensis Osb.)

    International Nuclear Information System (INIS)

    Nucellus of cultivar Valencia (Citrus Sinensis, Osb.) extracted from fruits 12 weeks after fertilization, were gamma irradiated (0.1, 1.0, 2.0, 4.0, 8.0 and 12.0 kR) before inoculation in culture media (pH 5.7) which comprised of macro and micronutrients of medium MS to which were added (in mg/l): mesoinusitol, 100; pyroxidin HCl, 1; nicotinic acid, 1; thiamine HCl, 0.2; malt extract, 500; sacarose, 50,000; agar-agar, 8,000. They were then Kept under 16 h light and 8h dark at a temperature of 270C. (M.A.C.)

  17. New acridone from the wood of Citrus reticulata Blanco.

    Science.gov (United States)

    Phetkul, Uraiwan; Wanlaso, Nutthakran; Mahabusarakam, Wilawan; Phongpaichit, Souwalak; Carroll, Anthony R

    2013-10-01

    A new acridone, named citruscridone (1) together with five known compounds were isolated from the wood of Citrus reticulata Blanco. Their structures were established based on spectroscopic evidence. The antibacterial and antifungal activities of the wood extracts and pure compounds were evaluated. PMID:23697332

  18. Cytological and molecular characterization of three gametoclones of Citrus clementina

    OpenAIRE

    GERMANA, MARIA ANTONIETTA; Aleza, Pablo; Carrera, Esther; Chen, Chunxian; Chiancone, Benedetta; Costantino, Gilles; Dambier, Dominique; Deng, Xiuxin; Federici, Claire T.; Froelicher, Yann; Guo, Wenwu; Ibanez, Victoria; Juarez, Jose; Kwok, Kevin; Luro, François

    2013-01-01

    Abstract Background Three gametoclonal plants of Citrus clementina Hort. ex Tan., cv. Nules, designated ESP, FRA, and ITA (derived from three labs in Spain, France, and Italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. The study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow c...

  19. Emergence and phylodynamics of Citrus tristeza virus in Sicily, Italy.

    Directory of Open Access Journals (Sweden)

    Salvatore Davino

    Full Text Available Citrus tristeza virus (CTV outbreaks were detected in Sicily island, Italy for the first time in 2002. To gain insight into the evolutionary forces driving the emergence and phylogeography of these CTV populations, we determined and analyzed the nucleotide sequences of the p20 gene from 108 CTV isolates collected from 2002 to 2009. Bayesian phylogenetic analysis revealed that mild and severe CTV isolates belonging to five different clades (lineages were introduced in Sicily in 2002. Phylogeographic analysis showed that four lineages co-circulated in the main citrus growing area located in Eastern Sicily. However, only one lineage (composed of mild isolates spread to distant areas of Sicily and was detected after 2007. No correlation was found between genetic variation and citrus host, indicating that citrus cultivars did not exert differential selective pressures on the virus. The genetic variation of CTV was not structured according to geographical location or sampling time, likely due to the multiple introduction events and a complex migration pattern with intense co- and re-circulation of different lineages in the same area. The phylogenetic structure, statistical tests of neutrality and comparison of synonymous and nonsynonymous substitution rates suggest that weak negative selection and genetic drift following a rapid expansion may be the main causes of the CTV variability observed today in Sicily. Nonetheless, three adjacent amino acids at the p20 N-terminal region were found to be under positive selection, likely resulting from adaptation events.

  20. Longevity of radiofrequency identification device microchips in citrus trees

    Science.gov (United States)

    Long-term identification of individual plants in the field is an important part of many types of botanical and horticultural research. In a previous report, we described methods for using implanted radiofrequency (RFID) microchips to tag citrus trees for field research. This report provides an upd...

  1. Citrus greening detection using airborne hyperspectral and multispectral imaging techniques

    Science.gov (United States)

    Hyperspectral imaging can provide unique spectral signatures for diseased vegetation. Airborne multispectral and hyperspectral imaging can be used to detect potentially infected trees over a large area for rapid detection of infected zones. This paper proposes a method to detect the citrus greening...

  2. Efficiency of chemotherapy coupled with thermotherapy against citrus HLB

    Science.gov (United States)

    Six independent experiments were carried out to evaluate the effectiveness of the chemotherapy coupled with the thermotherapy on pot-contained HLB-affected plants based on our previous results from graft-based methods. Three-year old potted HLB-affected citrus plants were exposed to 4 thermotherapy ...

  3. Micro-irrigation systems, automation and fertigation in citrus

    Directory of Open Access Journals (Sweden)

    Parameshwar Sidramappa Shirgure

    2012-11-01

    Full Text Available Citrus is number one group of fruits grown in more than 140 countries in the world. Micro-irrigation systems and fertigation management is one of the main concerns of the modem citrus fruit production irrespective of availability of soil, water and fertilizer resources. A variety of recommendations have emerged world over on irrigation systems and fertigation based on soil and leaf analysis of the nutrients, evapo-transpiration and water use pattern. The research review of literature has revealed best promising results on irrigation scheduling based on depletion pattern of soil available water content, irrigation systems and fertigation. Various micro-irrigation systems have established their superiority over traditionally used flood irrigation with micro-jets having little edge over rest of the others. Similarly, fertigation has shown good responses on growth, yield, quality and uniform distribution pattern of applied nutrients within the plant rootzone compared to band placement involving comparatively localized fertilization. Automated fertigation in citrus orchards is a new concept, which would be the only solitary choice amongst many irrigation monitoring methods in near future. The present status of the review on micro-irrigation and fertigation in citrus cultivars is clearly indicated in this article.

  4. Alternative methods for the control of postharvest citrus diseases.

    Science.gov (United States)

    Talibi, I; Boubaker, H; Boudyach, E H; Ait Ben Aoumar, A

    2014-07-01

    The postharvest diseases of citrus fruit cause considerable losses during storage and transportation. These diseases are managed principally by the application of synthetic fungicides. However, the increasing concern for health hazards and environmental pollution due to chemical use has required the development of alternative strategies for the control of postharvest citrus diseases. Management of postharvest diseases using microbial antagonists, natural plant-derived products and Generally Recognized As Safe compounds has been demonstrated to be most suitable to replace the synthetic fungicides, which are either being banned or recommended for limited use. However, application of these alternatives by themselves may not always provide a commercially acceptable level of control of postharvest citrus diseases comparable to that obtained with synthetic fungicides. To provide more effective disease control, a multifaceted approach based on the combination of different postharvest treatments has been adopted. Actually, despite the distinctive features of these alternative methods, several reasons hinder the commercial use of such treatments. Consequently, research should emphasize the development of appropriate tools to effectively implement these alternative methods to commercial citrus production.

  5. Larvicidal Activity of Citrus Limonoids against Aedes albopictus Larvae

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    Hazrat Bilal

    2012-12-01

    Full Text Available Background: Development of insecticide resistance occurred due to the continuous and misuse of synthetic insecticidestherefore, the recent study was conducted to explore eco-friendly plant extracts that have some potential to suppressmosquito larval population.Methods: WHO recommended mosquito larval bioassay method for insecticide was used while for the analysis of citrus oils for limonin and nomilin content HPLC was used.Results: Among the two citrus cultivars tested as larvicide against Aedes albopictus, valencia late (Citrus sinensis wasthe best in terms of LC50 (297 ppm, % mortality (97% and LT50 (18.49 hours then freutrall early (Citrus reticulatewith LC50 (377.4 ppm, % mortality (88% and LT50 (31 hours, While nomilin gave lowest LC50 (121.04 ppm than limonin (382.22 ppm after 72 hours of exposure. Valencia late also had more limonin and nomilin (377 μg/ml and 21.19 μg/ml than freutrall early (5.29 μg/ml and 3.89 μg/ml respectively.Conclusion: Valencia late showed best results in term of LC50, LT50 and percentage mortality against Aedes albopictus as it has more amount of nomilin then freutrall early, however further evaluation in the field conditions is required.

  6. Larvicidal Activity of Citrus Limonoids against Aedes Albopictus Larvae

    Directory of Open Access Journals (Sweden)

    Hazrat Bilal

    2012-12-01

    Full Text Available Background: Development of insecticide resistance occurred due to the continuous and misuse of synthetic insecti­cides therefore, the recent study was conducted to explore eco-friendly plant extracts that have some potential to sup­press mosquito larval population.Methods: World Health Organization recommended mosquito larval bioassay method for insecticide was used while for the analysis of citrus oils for limonin and nomilin content HPLC was used.Results: Among the two citrus cultivars tested as larvicide against Aedes albopictus, valencia late (Citrus sinensis was the best in terms of LC50 (297 ppm, % mortality (97% and LT50 (18.49 hours then freutrall early (Citrus reticulate with LC50 (377.4 ppm, % mortality (88% and LT50 (31 hours, While nomilin gave lowest LC­­50 (121.04 ppm than limonin (382.22 ppm after 72 hours of exposure. Valencia late also had more limonin and nomilin (377 µg/ml and 21.19 µg/ml than freutrall early (5.29 µg/ml and 3.89 µg/ml respectively.Conclusion: Valencia late showed best results in term of LC50, LT50 and percentage mortality against Aedes albopictus as it has more amount of nomilin then freutrall early, however further evaluation in the field conditions is required

  7. Inhibition of acetylcholinesterase activity by essential oil from Citrus paradisi.

    Science.gov (United States)

    Miyazawa, M; Tougo, H; Ishihara, M

    2001-01-01

    Inhibition of acetylcholinesterase (AChE) activity by essential oils of Citrus paradisi (grapefruit pink in USA) was studied. Inhibition of AChE was measured by the colorimetric method. Nootkatone and auraptene were isolated from C. paradisi oil and showed 17-24% inhibition of AChE activity at the concentration of 1.62 microg/mL. PMID:11858553

  8. Development of direct somatic embryogenesis and regeneration on citrus sinesis

    International Nuclear Information System (INIS)

    The plant regeneration processes in Citrus sinensis involves direct somatic embryogenesis. Culture medium used was MS basal supplemented with 50 mg/L sucrose, 0.27% agar and 0.1% vitamin at pH 5.8. Sucrose is the major carbon source for the induction of somatic embryo and also the maturation and germination of somatic embryo. (Author)

  9. Learning to fight a fly : developing citrus IPM in Bhutan

    NARCIS (Netherlands)

    Schoubroeck, van H.J.

    1999-01-01

    The chinese citrus fly is one of the key pests in Bhutanese mandarin orchards that lays eggs in developing fruit that cause pre-mature fruit drop. In this study it is used as a "model subject" to explore the integration of technical, social and administrative domains of knowledge. The confinement of

  10. 7 CFR 319.56-41 - Citrus from Peru.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Citrus from Peru. 319.56-41 Section 319.56-41 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Fruits and Vegetables § 319.56-41...

  11. Evaluation of citrus fibers as a tablet excipient.

    Science.gov (United States)

    Cespi, Marco; Bonacucina, Giulia; Roberts, Matthew; Hanson, Samuel; Jones, Stephen; Makevica, Elina; Casettari, Luca; Palmieri, Giovanni Filippo

    2014-04-01

    The consumption of fibers is associated with many health benefits, such as a reduction of cardiovascular and gastrointestinal diseases, control of body weight, and prevention of diabetes. Despite the widespread use of fiber supplements such as capsules or tablets, there is an almost complete lack of information concerning the technological properties of functional fibers used in nutraceutical formulations. The aim of this work was to characterize the technological properties of citrus fibers necessary for their use as a processing aid in tableting. The results obtained showed that citrus fibers share many properties of other polysaccharides used as tableting excipients, such as thermal behavior and compaction mechanism, together with an appreciable tabletability. However, the most interesting properties resulted from their disintegration power. Citrus fibers behaved in a similar manner to the well-known super disintegrant croscarmellose sodium and resulted to be little susceptible to their concentration, to lubricant type, and lubricant concentration. Thus, this work supports the idea of a potential use of citrus fibers as "active" substances and processing aid in the tableting of nutraceutical products and also as functional excipient in pharmaceutical tablets formulation.

  12. Physiological Mechanism on the Difference of Sugar Accumulation in Four Citrus Varieties

    Institute of Scientific and Technical Information of China (English)

    ZHAO Zhi-zhong; ZHANG Shang-long; LIU Shuan-tao; CHEN Jun-wei; TAO Jun

    2003-01-01

    The concentration of sucrose,glucose,fructose,titratable acid,ABA and the activity of acid invertase(AI)were measured injuice sacs of Bendizao mandarin(Citrus succosa Hort),Ponkan(Citrus reticulata Blanco),Miyagawa wase(Citrus unshiu Marc)and Meyer lemon(Citrus,meyeri Y).The acid hydrolysis of sucrose in vitro was also studied.The results showed that the sugar content,the ratio of different sugars,titratable acidity,ABA content and the activity of AI were obviously different in four citrus varieties.The sugar content inj uice sacs of citrus fruit was related to that of ABA at the late stage of fruit development.The result suggested that ABA played an important role in sugar accumulation in juice sacs of citrus fruit.Bendizao mandarin and Miyagawa wase mainly accumulated sucrose inj uice sacs of citrus fruit.Meyer lemon accumulated hexose,and Ponkan citrus was the sucrose-hexose accumulating type variety.In Meyer lemon with high acid degree,the composition of sugar inj uice sacs was determined by acid-hydrolysis of sucrose.In Bendizao mandarin,Ponkan citrus and Miyagawa wase with low acid degree,the composition of sugars at the late stage of fruit development was obviously affected by the AI activity.

  13. Desempenho da tangerineira 'Span Americana' em diferentes porta-enxertos

    Directory of Open Access Journals (Sweden)

    Simone Rodrigues da Silva

    2013-12-01

    Full Text Available Este trabalho objetivou avaliar o crescimento vegetativo, a produção e a qualidade de frutos da tangerina 'Span Americana' em diferentes porta-enxertos, nas condições edafoclimáticas de Bebedouro-SP. O plantio foi realizado em junho de 2003, em espaçamento de 6,0 m x 3,0 m, sendo utilizada irrigação por gotejamento a partir de 2006. Os porta-enxertos avaliados foram: citranges [Citrus sinensis (L. Osbeck × Poncirus trifoliata L. Raf] 'Carrizo' e 'Troyer', tetraploides; trifoliatas (P. trifoliata 'Davis A' e 'Flying Dragon'; limão Volkameriano Catania 2 (C. volkameriana Tenn. et Pasq., HRS 849 [(C. aurantium L. cv. 'Smooth Flat Seville' x P. trifoliata cv. 'Argentina'], tangelo 'Orlando' (C. reticulata Blanco × C. paradisi Macf. e limão 'Cravo' (C. limonia Osbeck. Foram avaliadas a produção acumulada, a eficiência produtiva e a precocidade de entrada em produção, no período de 2007 a 2009. Avaliaram-se, também, as dimensões das plantas e a taxa média de crescimento das plantas no período de 2005 a 2008, além da qualidade dos frutos em 2006 e 2007. Em pomares irrigados de tangerineira 'Span Americana', os porta-enxertos trifoliata 'Davis A' e HRS 849 apresentam desempenho horticultural satisfatório. Para plantio em alta densidade, a melhor performance da tangerineira 'Span Americana' é obtida com a utilização dos porta-enxertos trifoliata 'Flying Dragon' e citranges 'Troyer' e 'Carrizo'.

  14. Stylet morphometrics and citrus leaf vein structure in relation to feeding behavior of the Asian citrus psyllid Diaphorina citri, vector of citrus huanglongbing bacterium.

    Science.gov (United States)

    Ammar, El-Desouky; Hall, David G; Shatters, Robert G

    2013-01-01

    The Asian citrus psyllid (ACP), Diaphorina citri (Hemiptera: Psyllidae), is the primary vector of the phloem-limited bacterium Candidatus Liberibacter asiaticus (LAS) associated with huanglongbing (HLB, citrus greening), considered the world's most serious disease of citrus. Stylet morphometrics of ACP nymphs and adults were studied in relation to citrus vein structure and to their putative (histologically verified) feeding sites on Valencia orange leaves. ACP nymphs preferred to settle and feed on the lower (abaxial) side of young leaves either on secondary veins or on the sides of the midrib, whereas adults preferred to settle and feed on the upper (adaxial) or lower secondary veins of young or old leaves. Early instar nymphs can reach and probe the phloem probably because the distance to the phloem is considerably shorter in younger than in mature leaves, and is shorter from the sides of the midrib compared to that from the center. Additionally, the thick-walled 'fibrous ring' (sclerenchyma) around the phloem, which may act as a barrier to ACP stylet penetration into the phloem, is more prominent in older than in younger leaves and in the center than on the sides of the midrib. The majority (80-90%) of the salivary sheath termini produced by ACP nymphs and adults that reached a vascular bundle were associated with the phloem, whereas only 10-20% were associated with xylem vessels. Ultrastructural studies on ACP stylets and LAS-infected leaves suggested that the width of the maxillary food canal in first instar nymphs is wide enough for LAS bacteria to traverse during food ingestion (and LAS acquisition). However, the width of the maxillary salivary canal in these nymphs may not be wide enough to accommodate LAS bacteria during salivation (and LAS inoculation) into host plants. This may explain the inability of early instar nymphs to transmit LAS/HLB in earlier reports.

  15. Stylet morphometrics and citrus leaf vein structure in relation to feeding behavior of the Asian citrus psyllid Diaphorina citri, vector of citrus huanglongbing bacterium.

    Directory of Open Access Journals (Sweden)

    El-Desouky Ammar

    Full Text Available The Asian citrus psyllid (ACP, Diaphorina citri (Hemiptera: Psyllidae, is the primary vector of the phloem-limited bacterium Candidatus Liberibacter asiaticus (LAS associated with huanglongbing (HLB, citrus greening, considered the world's most serious disease of citrus. Stylet morphometrics of ACP nymphs and adults were studied in relation to citrus vein structure and to their putative (histologically verified feeding sites on Valencia orange leaves. ACP nymphs preferred to settle and feed on the lower (abaxial side of young leaves either on secondary veins or on the sides of the midrib, whereas adults preferred to settle and feed on the upper (adaxial or lower secondary veins of young or old leaves. Early instar nymphs can reach and probe the phloem probably because the distance to the phloem is considerably shorter in younger than in mature leaves, and is shorter from the sides of the midrib compared to that from the center. Additionally, the thick-walled 'fibrous ring' (sclerenchyma around the phloem, which may act as a barrier to ACP stylet penetration into the phloem, is more prominent in older than in younger leaves and in the center than on the sides of the midrib. The majority (80-90% of the salivary sheath termini produced by ACP nymphs and adults that reached a vascular bundle were associated with the phloem, whereas only 10-20% were associated with xylem vessels. Ultrastructural studies on ACP stylets and LAS-infected leaves suggested that the width of the maxillary food canal in first instar nymphs is wide enough for LAS bacteria to traverse during food ingestion (and LAS acquisition. However, the width of the maxillary salivary canal in these nymphs may not be wide enough to accommodate LAS bacteria during salivation (and LAS inoculation into host plants. This may explain the inability of early instar nymphs to transmit LAS/HLB in earlier reports.

  16. Role of imidacloprid in integrated pest management of California citrus.

    Science.gov (United States)

    Grafton-Cardwell, E E; Lee, J E; Robillard, S M; Gorden, J M

    2008-04-01

    Portions of three commercial citrus orchards were treated for 1 yr with foliar imidacloprid or for 2 yr with a systemic formulation in a replicated plot design to determine the impact of this neonicotinoid on the San Joaquin Valley California citrus integrated pest management (IPM) program. Foliar-applied imidacloprid had little effect on California red scale, Aonidiella aurantii (Maskell); cottony cushion scale, Icerya purchasi Maskell; or citrus red mite, Panonychus citri (McGregor), populations. Short-term suppression of the parasitoids Aphytis melinus DeBach and Comperiella bifasciata Howard; vedalia, Rodolia cardinalis (Mulsant); and the predacious mite Euseius tularensis (Congdon) were observed. Suppression of natural enemies allowed scales and mites to maintain higher populations in the treated areas compared with the nontreated areas. Thus, foliar imidacloprid did not exhibit control of these citrus pest species, and it disrupted biological control. Systemically applied imidacloprid suppressed California red scale and citricola scale populations 2-3 mo after treatment. Suppression of parasitoids of the California red scale also was observed. Thus, treatments of systemic imidacloprid applied in areawide management programs for invasive pests would provide a benefit of California red scale and citricola scale suppression. However, this treatment provided only single-season control of citricola scale, it was somewhat disruptive of biological control, and it did not suppress densities of either scale as low as a treatment of the organophosphate chlorpyrifos for citricola scale or the insect growth regulator pyriproxyfen for California red scale. Insecticides with longer periods of efficacy and greater IPM compatibility than imidacloprid should be used for a sustainable IPM approach in California citrus. PMID:18459411

  17. MICROWAVE-ASSISTED EXTRACTION OF ESSENTIAL OIL FROM CITRUS GRANDIS PEEL%微波辅助提取柚皮精油的研究

    Institute of Scientific and Technical Information of China (English)

    龚盛昭; 程江; 杨卓如

    2005-01-01

    进行了微波辅助提取柚皮精油的实验.结果表明,石油醚是微波辅助提取柚皮精油的最佳溶剂,液料质量比、物料含水率、粒度、微波功率等因素对微波辅助提取柚皮精油有较大影响.通过实验得到了柚皮精油提取的最佳工艺条件:物料粒度60目,物料含水率7.4%,总液料质量比14:1,微波功率300W,提取两次,每次微波辐射时间3 min.提取液进行真空浓缩至不再有溶剂蒸出,得精油粗产品.往精油粗产品中加入乙醇溶解,放入-15℃冰箱中冷冻48 h,离心分离去除树脂状物质,过滤,滤液进行真空浓缩后,即得精油,产率约2.09%,精油纯度99.0%.与传统的直接加热提取法相比,微波辅助提取法所用时间仅为直接加热提取法的1/20.%Microwave-assisted extraction(MAE) was used to extract essential oil (EO) from pomelo(Citrus grandis (L.)Osbeck) peel (CGP). Results show that petroleum ether is an optimal solvent and the extraction was affected mainly by stock mass ratio, microwave power, water content and granularity of material. The optimal conditions were obtained. Materials with granularity of 60 mesh and water content of around 7.4 % are suitablefor extraction. Total solvent-material mass ratio is 14: 1. Extraction had to be performed twice with microwave irradiation (300 W) for 3 min each time. Crude EO was obtained after the extracted liquor was concentrated under vacuum. Crude EO was then dissolved with ethanol, frozen at - 15 ℃ for 48 h, and centrifugally separated and filtered to get rid of impurities. The final product EO with yield of 2.09 % and purity of 99.0 % was then obtained after distilling the liquor under vacuum. Compared with direct-heating extraction (DHE), MAE reduced the extracting time up to 1/20.

  18. Análise espaço-temporal da clorose variegada dos citros no Noroeste do Paraná, com uso de PCR para detecção de Xylella fastidiosa = Spatio-temporal analysis of the citrus variegated chlorosis (CVC in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa

    Directory of Open Access Journals (Sweden)

    William Mário de Carvalho Nunes

    2006-07-01

    Full Text Available A citricultura é afetada por inúmeras doenças, como a clorose variegada do citros (CVC, causada pela bactéria Xylella fastidiosa. O objetivo deste trabalho foi determinar a distribuição espacial da doença dentro de pomares comerciais do Noroeste do Paraná com o uso de métodos moleculares. Foram selecionados pomares sintomáticos para CVC com as variedades ‘Pêra’, ‘Valência’ e ‘Folha Murcha’ (Citrus sinensis Osbeck. Foram marcadas para cada variedade, 4 plantas-referência positivas para CVC (por sintomas e análise molecular e 8 plantas ao redor de cada uma das plantas-referência foram amostradas, num total de 36 plantas por variedade. Realizou-se o teste da Reação da Polimerase em Cadeia (PCR para detecção da bactéria e na mesma época foram conduzidas avaliações visuais de sintomas de CVC. Os resultados da análise temporal, utilizando-se os modelos Monomolecular, Logístico e Gompertz, apontaram o modelo Logístico como o que melhor se ajustou para descrever o comportamento da doença no tempo, para todas as variedades estudadas. Observou-se que o comportamento espacial da doença diferiu quando a mesma área foiavaliada pelos métodos visual e molecular, resultando em uma diferença no padrão espacial das áreas avaliadas. Portanto, ambos os métodos empregados, sintomas e PCR, foram capazes de constatar asmudanças no padrão espacial apresentado, sendo que a análise molecular (PCR foi mais sensível para detectar as mudanças ocorridas.Countless diseases affect the citriculture, as the citrus variegated chlorosis (CVC which is caused by the bacteria Xylella fastidiosa.The aim of this work was to determine the space distribution of the disease inside commercial orchards in the Northwest of Paraná, using molecular methods. Symptomatic orchards were selected for CVC with the varieties 'Pêra', 'Valência' and 'Folha Murcha' (Citrus sinensis Osbeck. For each variety, 4 positive reference-plant for CVC

  19. Citrus asymmetric somatic hybrids produced via fusion of gamma-irradiated and iodoacetamide-treated protoplasts Híbridos somáticos assimétricos de citros produzidos pela fusão de protoplastos irradiados e tratados com iodoacetamida

    Directory of Open Access Journals (Sweden)

    Claudine Maria de Bona

    2009-05-01

    Full Text Available The objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma-irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad. cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck 'Itaboraí', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow. and 'Changsha' mandarin (C. reticulata Blanco and 'Murcott' tangor (C. reticulata x C. sinensis. Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L-1 iodoacetamide (IOA, and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto sour orange, rough lemon or 'Swingle' (C. paradisi x Poncirus trifoliata x 'Sunki' mandarin rootstock seedlings, or rooted after dipping their bases in indol-butyric acid (IBA solution. The products were later acclimatized to greenhouse conditions. Ploidy was analyzed by flow cytometry, and hybridity was confirmed by amplified fragment length polymorphism (AFLP analysis of plantlet DNAsamples. The best treatment was the donor-recipient fusion combination of 80 Gy-irradiated 'Ruby Red' protoplasts with 20 min IOA-treated 'Succari' protoplasts. Tetraploid and aneuploid plants were produced. Rooting recalcitrance was solved by dipping shoots' stems in 3,000 mg L-1 IBA solution for 10 min.O objetivo deste trabalho foi produzir híbridos somáticos assimétricos de citros pela fusão de protoplastos irradiados com raios gama e protoplastos tratados com iodoacetamida. Protoplastos foram isolados de suspensões celulares embriogênicas de pomelo (Citrus paradisi Macfad., cultivares Ruby Red e Flame, de laranja doce (C. sinensis Osbeck 'Itaboraí', 'Natal', Valencia' e 'Succari', de tangerinas 'Satsuma' (C. unshiu Marcow. e 'Changsha' (C. reticulata Blanco e de tangor 'Murcott' (C. reticulata x C. sinensis

  20. [Pharmacognostic studies on the peel of Citrus reticulata Blanco cv. zhangshuensis and Citrus reticulata Blanco Var. kinokuni (Tanaka) H. H. Hu produced in Jiangxi].

    Science.gov (United States)

    Fang, C S; Wang, A S

    1989-10-01

    Pharmacognostical studies on the peel of Citrus reticulata cv. zhangshuensis and Citrus reticulata var. kinokuni have been carried out in comparison with four crude drugs of Chenpi. Information on the research of resources of these two drugs is provided. PMID:2597317