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Sample records for cinnamomun zeylanicum mentha

  1. Free Radicals Scavenging Activity of Essential Oils and Different Fractions of Methanol Extract of Zataria Multiflora, Salvia Officinalis, Rosmarinus Officinalis, Mentha Pulegium and Cinnamomum Zeylanicum

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    S Changizi Ashtiani

    2012-04-01

    Full Text Available Introduction: Essential oils and extracts from medicinal plants are regarded as natural food preservatives and health promoting drugs. Considering their antioxidant activity, most of them can prevent oxidative stress. The present study was aimed to evaluate free radicals scavenging activity of essential oils and different fractions of methanol extracts from cinnamon, pennyroyal, black cumin, sage, rosemary and azkand. Methods: Antioxidant property of essential oils and different fractions of these medicinal plants was studied by determining their DPPH(2,2-diphenyl-1-picrylhydrazyl free radicals scavenging activity. Results: There were significant differences among the free radical scavenging activity of studied essential oils and different fractions. Ethyl acetate fractions were identified as the most active fractions than other ones and even synthetic antioxidant (BHT, IC50 value of 239.5μg/ml with the highest activity in Mentha pulegium (47.2 μg/ml μg/ml. Among others, n-hexane fraction of rosemary (969 μg/ml, dichloromethane fraction of rosemary (205.46 μg/ml and zatar (344 μg/ml and aqueous fractions of cinnamon (117.6 μg/ml and sage (321.3 μg/ml exhibited appreciable antioxidant activity. Conclusion: Regarding considerable activity of studied extracts, they have the potential to be used as natural antioxidants in relevant industries.

  2. Extraction of Essential Oil from Cinnamon (Cinnamomum Zeylanicum)

    OpenAIRE

    Wong, Y C; M. Y. Ahmad-Mudzaqqir; W.A. Wan-Nurdiyana

    2014-01-01

    Cinnamomum zeylanicum is one of the herbs and spices plants that come from cinnamon family which contains high quality of essential oil. In this study, the essential oil from plant Cinnamomum zeylanicum was extracted using two methods which were steam distillation and Soxhlex extraction. Steam distillation produced high quality essential oil extraction using separatory funnel. Soxhlet extraction produced essential oil in crude form using rotary evaporator to purify the extracted product. Cinn...

  3. Essential oils of Mentha pulegium and Mentha rotundifolia from Uruguay

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    Daniel Lorenzo

    2002-12-01

    Full Text Available Essential oils obtained by hydrodistillation from leaves of Mentha pulegium L. and Mentha rotundifolia (L. Huds. from Uruguay were analysed by GC-FID and GC-MS. Oxygen-containing monoterpenes were the main group of constituents in both oils. Pulegone, isomenthone and menthone were the major components in the oil of M. pulegium, whereas piperitenone oxide and (Z-sabinene hydrate were the major ones in M. rotundifolia. Enantiomerically pure (--menthone, (+-isomenthone, (+-isomenthol, (--menthol and (+-pulegone were detected by multidimensional gas chromatography in the case of M. pulegium oil.Óleos essenciais obtidos por hidrodestilação das folhas de Mentha pulegium L. e Mentha rotundifolia (L. Huds. do Uruguay foram analisados por GC-FID e GC-MS. O grupo de monoterpenes oxigenados foi o mais importante em ambos os óleos, sendo que a pulegona, isomenthona e menthona foram os constituintes maioritarios no óleo de Mentha pulegium, no entanto, o ôxido de piperitenona e (Z-hidrato de sabineno foram os maioritarios na Mentha rotundifolia. (--mentone, (+-isomentone, (+-isomenthol, (--menthol e (+-pulegone enantioméricamente puras foram detectadas por cromatografía gasosa multidimensional no caso do óleo de Mentha pulegium.

  4. Extraction of Essential Oil from Cinnamon (Cinnamomum Zeylanicum

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    Y.C.Wong

    2014-03-01

    Full Text Available Cinnamomum zeylanicum is one of the herbs and spices plants that come from cinnamon family which contains high quality of essential oil. In this study, the essential oil from plant Cinnamomum zeylanicum was extracted using two methods which were steam distillation and Soxhlex extraction. Steam distillation produced high quality essential oil extraction using separatory funnel. Soxhlet extraction produced essential oil in crude form using rotary evaporator to purify the extracted product. Cinnamon essential oil contains high cinnamaldehyde content which is the main component in cinnamon. The percentage of cinnamaldehyde in essential oil from steam distillation was about 90% and 62-73% from Soxhlet extraction. Cinnamon essential oil has high antimicrobial properties which formed clear zone when tested with gram positive bacteria Bacillus subtilis s.p and a gram negative bacterium Escherichia coli. It also showed antimicrobial properties with two unknown bacteria with unknown characteristics. Cinnamaldehyde contains high antibiotic quality since it is the main compound in cinnamon.

  5. Chemistry, biogenesis, and biological activities of Cinnamomum zeylanicum.

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    Jayaprakasha, G K; Rao, L Jagan Mohan

    2011-07-01

    The genus Cinnamomum comprises of several hundreds of species, which are distributed in Asia and Australia. Cinnamomum zeylanicum, the source of cinnamon bark and leaf oils, is an indigenous tree of Sri Lanka, although most oil now comes from cultivated areas. C. zeylanicum is an important spice and aromatic crop having wide applications in flavoring, perfumery, beverages, and medicines. Volatile oils from different parts of cinnamon such as leaves, bark, fruits, root bark, flowers, and buds have been isolated by hydro distillation/steam distillation and supercritical fluid extraction. The chemical compositions of the volatile oils have been identified by GC and GC-MS. More than 80 compounds were identified from different parts of cinnamon. The leaf oil has a major component called eugenol. Cinnamaldehyde and camphor have been reported to be the major components of volatile oils from stem bark and root bark, respectively. Trans-cinnamyl acetate was found to be the major compound in fruits, flowers, and fruit stalks. These volatile oils were found to exhibit antioxidant, antimicrobial, and antidiabetic activities. C. zeylanicum bark and fruits were found to contain proanthocyandins with doubly linked bis-flavan-3-ol units in the molecule. The present review provides a coherent presentation of scattered literature on the chemistry, biogenesis, and biological activities of cinnamon. PMID:21929331

  6. De vluchtige olie van enkele chemotypen van mentha suaveolens EHRH. en van hybriden met mentha longifolia (L.) HUDSON

    NARCIS (Netherlands)

    Hendriks, Hindrik

    1974-01-01

    De opvatting dat Mentha x piperita L. een bastaard zou zijn van Mentha spicata L. en Mentha aquatica L. werd nader besproken. Hierbij werd Mentha spicata beschouwd als een bastaard van Mentha longifolia (L.) HUDSON en Mentha suaveolens EHRH. ... Zie: Samenvatting.

  7. Essential oils of Mentha pulegium and Mentha rotundifolia from Uruguay

    OpenAIRE

    Daniel Lorenzo; Daniel Paz; Eduardo Dellacassa; Philip Davies; Roser Vila; Salvador Cañigueral

    2002-01-01

    Essential oils obtained by hydrodistillation from leaves of Mentha pulegium L. and Mentha rotundifolia (L.) Huds. from Uruguay were analysed by GC-FID and GC-MS. Oxygen-containing monoterpenes were the main group of constituents in both oils. Pulegone, isomenthone and menthone were the major components in the oil of M. pulegium, whereas piperitenone oxide and (Z)-sabinene hydrate were the major ones in M. rotundifolia. Enantiomerically pure (-)-menthone, (+)-isomenthone, (+)-isomenthol, (-)-m...

  8. ETHNOBOTANY, PHYTOCHEMICAL AND PHARMACOLOGICAL ASPECTS OF CINNAMOMUM ZEYLANICUM BLUME.

    OpenAIRE

    Das Manosi; Mandal Suvra; Mallick Budhimanta; Hazra Jayram

    2013-01-01

    Cinnamomum zeylanicum, popularly known as cinnamon or ceylon cinnamon refers to the tropical evergreen tree as well as the bark that is extracted from the plant. Cinnamon are recognized for its sweet flavour and aroma and used as an important spice in India in almost every delicious dishes .The spice has gained more importance due to its well uses as remedy for the treatment of various types of disorders in the Ayurvedic and folklore system of medicine in India from the era of “Charaka samhi...

  9. Spectroscopic study of Mentha oils

    Science.gov (United States)

    Rai, A. K.; Singh, A. K.

    The visible fluorescence and excitation spectra of Mentha oils (Japanese mint oil, peppermint oil and spearmint oil) have been recorded. Different physical constants which are characteristic of the fluorescent molecules have been calculated for all three oils. Results reveal that the same group of organic compounds dominate in the oils of peppermint and spearmint, whereas some different compound is present in Japanese mint oil. It is also found that the fluorescence intensity of these oils is comparable to that of Rhodamine 6G dye in methanol solution. Our studies suggest that Mentha oils may be a useful lasing material in the 450-600 nm wavelength range.

  10. Inhibitory potential of nine mentha species against pathogenic bacteria strains

    International Nuclear Information System (INIS)

    Plants produce secondary metabolites, which are used in their growth and defense against pathogenic agents. These plant based metabolites can be used as natural antibiotics against pathogenic bacteria. Synthetic antibiotics caused different side effects and become resistant to bacteria. Therefore the main objective of the present study was to investigate the inhibitory potential of nine Mentha species extracts against pathogenic bacteria. The methanolic leaves extracts of nine Mentha species (Mentha arvensis, Mentha longifolia, Mentha officinalis, Mentha piperita, Mentha citrata, Mentha pulegium, Mentha royleana, Mentha spicata and Mentha suareolens) were compared for antimicrobial activities. These Mentha species showed strong antibacterial activity against four microorganisms tested. Mentha arvensis showed 25 mm and 30 mm zones of inhibition against Staphylococcus aureus, Vibrio cholera and Enterobacter aerogens. Moreover, Mentha longifolia showed 24 mm zone of inhibition against Staphylococcus aureus. Mentha officinalis showed 30 mm zone of inhibition against Staphylococcus aureus. 25 mm inhibitory zone was recorded against Staphylococcus aureus by Mentha piperita. Mentha royleana showed 25 mm zone of inhibition against Vibrio cholera, while Mentha spicata showed 21 mm, 22 mm and 23 mm zones of inhibition against Staphylococcus aureus, Vibrio cholera and Enterobacter aerogens. Moreover most of the Mentha species showed zone of inhibition in the range of 10-20 mm. (author)

  11. Green synthesis of gold nanoparticles using Cinnamomum zeylanicum leaf broth

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    Smitha, S. L.; Philip, Daizy; Gopchandran, K. G.

    2009-10-01

    Development of biologically inspired experimental processes for the synthesis of nanoparticles is an important branch of nanotechnology. The synthesis of gold nanoparticles using Cinnamomum zeylanicum leaf broth as the reducing agent is reported. The morphology of the particles formed consists of a mixture of gold nanoprisms and spheres with fcc (1 1 1) structure of gold. At lower concentrations of the extract, formation of prism shaped Au particles dominates, while at higher concentrations almost spherical particles alone are observed. Good crystallinity of the nanoparticles with fcc phase is evident from XRD patterns, clear lattice fringes in the high resolution TEM image and bright circular rings in the SAED pattern. Au nanoparticles grown are observed to be photoluminescent and the intensity of photoemission is found to increase with increase in leaf broth concentration. The ability to modulate the shape of nanoparticles as observed in this study for gold nanoparticles opens up the exciting possibility of developing further synthetic routes employing ecofriendly sources.

  12. Free radical scavenging (DPPH) potential in nine Mentha species.

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    Ahmad, Nisar; Fazal, Hina; Ahmad, Iftikhar; Abbasi, Bilal Haider

    2012-02-01

    Mentha species are used in every day life in various food items. These species produce valuable secondary metabolites that scavenge toxic free radicals. Toxic free radicals can cause different diseases in the human body. In the present study free radical scavenging potential (1,1-diphenyl-2-picrylhydrazyl scavenging activity) in nine Mentha species were investigated to evaluate and explore new potential sources for natural antioxidants. The activity was performed after different time intervals with incubation period of 30 minutes. The methanolic extracts revealed that significantly higher activity (82%) was observed in Mentha suaveolens, followed by Mentha longifolia (79%), Mentha officinalis (76%) and Mentha piperita, Mentha pulegium, Mentha royleana (75%), respectively. Significantly same activity was observed in Mentha arvensis and Mentha spicata. Lower activity was observed in Mentha citrata (64%). The present study revealed that these species can be used as natural antioxidants. PMID:21646282

  13. Free radical scavenging (DPPH) potential in nine Mentha species.

    Science.gov (United States)

    Ahmad, Nisar; Fazal, Hina; Ahmad, Iftikhar; Abbasi, Bilal Haider

    2012-02-01

    Mentha species are used in every day life in various food items. These species produce valuable secondary metabolites that scavenge toxic free radicals. Toxic free radicals can cause different diseases in the human body. In the present study free radical scavenging potential (1,1-diphenyl-2-picrylhydrazyl scavenging activity) in nine Mentha species were investigated to evaluate and explore new potential sources for natural antioxidants. The activity was performed after different time intervals with incubation period of 30 minutes. The methanolic extracts revealed that significantly higher activity (82%) was observed in Mentha suaveolens, followed by Mentha longifolia (79%), Mentha officinalis (76%) and Mentha piperita, Mentha pulegium, Mentha royleana (75%), respectively. Significantly same activity was observed in Mentha arvensis and Mentha spicata. Lower activity was observed in Mentha citrata (64%). The present study revealed that these species can be used as natural antioxidants.

  14. Studies on antimicrobial activities of solvent extracts of different spices.

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    Keskin, Dilek; Toroglu, Sevil

    2011-03-01

    The antimicrobial activities of the ethyl acetate, acetone and methanol extract of 12 plant species were studied. The extract of Capsicum annuum (red pepper) (fruit) Zingiber officinale (ginger) (root), Cuminum cyminum (cumin), Alpinia ficinarum (galingale), Coriandrum sativum (coriander), Cinnamomun zeylanicum Nees (cinnamomun), Origanum onites L. (thyme), Folium sennae (senna), Eugenia caryophyllata (cloves), Flos tiliae (lime), Folium menthae crispae (peppermint) and Piper nigrum (blackpepper) were tested in vitro against 2 fungi and 8 bacterial species by the disc diffusion method. Klebsiella pneumonia 13883, Bacillus megaterium NRS, Pseudomonas aeroginosa ATCC 27859, Staphylococcus aureus 6538 P, Escherichia coil ATCC 8739, Enterobacter cloaca ATCC 13047, Corynebacterium xerosis UC 9165, Streptococcus faecalis DC 74, Kluyveromyces marxianus, Rhodotorula rubra were used in this investigation. The results indicated that extracts of different spices has shown antibacterial activity in the range of 7-24 mm 30 microl(-1) inhibition zone Eugenia caryophyllata (clove), 7-20 mm 30microl(-1) inhibition zone Capsicum annum (red pepper) and Cinnamomun zeylanicum (cinnamon) bark, 7-18 mm 30microl(-1) inhibition zone Folium sennae (senna) leaves, 7-16 mm 30 microl(-1) inhibition zone Zingiber officinale (ginger) root, 7-15 mm 30microl(-1) inhibition zone Cuminum cyminum (cumin) seed, 7-14 mm 30 microl(-1) inhibition zone Folium menthae crispae (peppermint), Origanum onites (thyme) leaves and Alpinia ficinarum (galingale) root, 7-12 mm 30 microl(-1) inhibiton zone Piper nigrum (blackpepper), 7-11 mm 30microl(-1) inhibition zone Flos tiliae (lime) leaves, 7-8 mm 30microl(-1) inhibition zone Coriandrum sativum (coriander) to the microorganisms tested.

  15. ETHNOBOTANY, PHYTOCHEMICAL AND PHARMACOLOGICAL ASPECTS OF CINNAMOMUM ZEYLANICUM BLUME.

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    Das Manosi

    2013-04-01

    Full Text Available Cinnamomum zeylanicum, popularly known as cinnamon or ceylon cinnamon refers to the tropical evergreen tree as well as the bark that is extracted from the plant. Cinnamon are recognized for its sweet flavour and aroma and used as an important spice in India in almost every delicious dishes .The spice has gained more importance due to its well uses as remedy for the treatment of various types of disorders in the Ayurvedic and folklore system of medicine in India from the era of “Charaka samhita”. The herb is used for the treatment of dyspeptic conditions, flatulence, loss of appetite, abdominal pain with diarrhoea, inflammation of the eye, leukorrhoea, vaginitis, rheumatism, neuralgia, wounds, and toothache. The oil isolated from the different parts of the plant containing cinnamaldehyde and eugenol as major constituents are considered as active principle of cinnamon. Over the past two decades many scientific journals are describing its nutritional and medicinal properties. This review has tried to include an up to date phytochemical and biological research on cinnamon. The ethnobotanical uses have also been discussed.

  16. Total phenolic content and antioxidant activity of six wild Mentha species (Lamiaceae) from northeast of Algeria

    OpenAIRE

    Amina Benabdallah; Chaabane Rahmoune; Mahieddine Boumendjel; Oumayma Aissi; Chokri Messaoud

    2016-01-01

    Objective: To investigate the total phenolics, flavonoids and tannins content and the in vitro antioxidant activities of methanolic extracts of six wild Mentha species which are Mentha aquatica, Mentha arvensis, Mentha piperita, Mentha pulegium, Mentha rotundifolia and Mentha villosa. Methods: The Folin–Ciocalteu method was used to determine the total phenols content while flavonoids were estimated according to the aluminum chloride colorimetric method. To evaluate tannins content, vanilli...

  17. Control of Aspergillus flavus Growth in Tomato Paste by Cinnamomum zeylanicum and Origanum vulgare L. Essential Oils

    OpenAIRE

    F Kalantary

    2014-01-01

    This study was conducted to evaluate the antifungal activities of cinnamon (Cinnamomum zeylanicum) and oregano (Origanum vulgare L.) essential oils against Aspergillus flavus in culture media and tomato paste. The chemical compositions of the essential oils were determined by Gas Chromatography-Mass Spectroscopy (GC-MS). Trans- cinnamaldehyde was found to be the main constituent of Cinnamomum zeylanicum essential oil (CZEO), followed by methyl eugenol, δ- cadinene and γ- cadinene. The major c...

  18. Final report on the safety assessment of Mentha Piperita (Peppermint) Oil, Mentha Piperita (Peppermint) Leaf Extract, Mentha Piperita (Peppermint) Leaf, and Mentha Piperita (Peppermint) Leaf Water.

    Science.gov (United States)

    Nair, B

    2001-01-01

    Mentha Piperita (Peppermint) Oil, Mentha Piperita (Peppermint) Leaf Extract, Mentha Piperita (Peppermint) Leaf, Mentha Piperita (Peppermint) Leaf Water are obtained from the Mentha piperita plant. The oil is currently used in cosmetic formulations as a fragrance component, but previously had been also described as a denaturant. The extract and leaves are described as biological additives, but only the extract is reported to be used. Peppermint Water is described as a flavoring agent or fragrance component, but is not currently in use. Peppermint Oil is used at a concentration of Oil is composed primarily of menthol and menthone. Other possible constituents include pulegone, menthofuran, and limone. Most of the safety test data concern Peppermint Oil. The oil is considered to present the "worst case scenario" because of its many constituents, so data on the oil were considered relevant to the entire group of ingredients. Peppermint Oil was minimally toxic in acute oral studies. Short-term and sub-chronic oral studies reported cystlike lesions in the cerebellum in rats that were given doses of Peppermint Oil containing pulegone, pulegone alone, or large amounts (>200 mg/kg/day) of menthone. Pulegone is also a recognized hepatotoxin. Repeated intradermal dosing with Peppermint Oil produced moderate and severe reactions in rabbits, although Peppermint Oil did not appear to be phototoxic. Peppermint Oil was negative in the Ames test and a mouse lymphoma mutagenesis assay but gave equivocal results in a Chinese hamster fibroblast cell chromosome aberration assay. In a carcinogenicity study of toothpaste and its components, no apparent differences were noted between mice treated with Peppermint Oil and those treated with the toothpaste base. Isolated clinical cases of irritation and/or sensitization to Peppermint Oil and/or its constituents have been reported, but Peppermint Oil (8%) was not a sensitizer when tested using a maximization protocol. It was expected that

  19. Dessication tolerance of Cinnamomum zeylanicum Ness SeedsTolerância à dessecação de sementes de Cinnamomum zeylanicum Ness

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    Leandra Matos Barroso

    2012-05-01

    Full Text Available In order to evaluate the physiological quality of seeds of Cinnamomum zeylanicum Ness, submitted to different levels of drying, the fruits of dark brown and fully mature were collected and washed by hand, then the seeds were exposed to drying, on paper towel, under laboratory conditions. Treatments consisted of sampling for the determination of water content, germination and vigor tests at the beginning of the experiment and after 12, 24, 36, 48, 60, 72, 84 and 96 hours. The experimental design was completely randomized with four replications of 25 seeds each. The data were subjected to analysis of variance and polynomial regression. Cinnamon seeds are dispersed with high water content (39.43% and viability is compromised when dehydrated, the germination capacity as well as its speed decreases with dehydration, characterizing the recalcitrant behavior of seeds, with water content 34.82% less than both the percentage and the speed of germination are reduced. The seeds of C. zeylanicum tolerate desiccation to 12 hours without significant loss of vigor. Com o objetivo de avaliar a qualidade fisiológica das sementes de canela (Cinnamomum zeylanicum Ness submetidas a diferentes níveis de secagem, os frutos de coloração marrom escura e completamente maduros foram coletados e despolpados manualmente, em seguida, as sementes foram expostas para a secagem, sobre papel toalha, em condições de laboratório. Os tratamentos consistiram na amostragem para a determinação do teor de água, testes de germinação e vigor no início do experimento e após 12, 24, 36, 48, 60, 72, 84 e 96 horas. O delineamento experimental utilizado foi o inteiramente ao acaso, em quatro repetições de 25 sementes cada. Os dados obtidos foram submetidos à análise de variância e de regressão polinomial. As sementes de canela são dispersas com alto teor de água (39,43% e tem a viabilidade comprometida quando desidratadas; a capacidade de germinação bem como a sua

  20. Constituintes voláteis das folhas e dos galhos de Cinnamomum zeylanicum Blume (Lauraceae) Volatile constituents from leaves and branches of Cinnamomum zeylanicum Blume (Lauraceae)

    OpenAIRE

    Maria da Paz Lima; Maria das Graças B. Zoghbi; Eloisa Helena A. Andrade; Tatiana Maria D. Silva; Carlos Sousa Fernandes

    2005-01-01

    Os óleos essenciais das folhas e dos galhos de Cinnamomum zeylanicum coletada no município de Manaus, estado do Amazonas, foram obtidos por hidrodestilação e analisados através de CG-EM. Vinte e três constituintes foram identificados nas folhas, dos quais o eugenol foi o que apresentou maior porcentagem (60%). Nos galhos foram identificados trinta e seis componentes, com predominância dos monoterpenos alfa- e beta-pineno (9,9%; 3,5%), alfa-felandreno (9,2%), p-cimeno (6,2%), limoneno (7,9%), ...

  1. [Phenotypic and phytochemical differences between Mentha arvensis L. and Mentha canadiensis L].

    Science.gov (United States)

    Shelepova, O V; Voronkova, T V; Kondrat'eva, V V; Semenova, M V; Bidiukova, G F; Olekhnovich, L S

    2014-01-01

    A taxonomic study of anatomical, morphological, and phytochemical characteristics of Mentha arvensis L. and Mentha canadiensis L. using hierarchical cluster analysis has been conducted and the differences between the species studied have been revealed. The ratio between the lengths of the calyx tube and the calyx lobes, the number of secretory glands on the upper and lower surfaces of the leaf, and the composition of the essen- tial oil were shown to be the most appropriate parameters for classification.

  2. Supercritical CO2 extraction of mentha (Mentha piperita L.) at different solvent densities

    OpenAIRE

    DUŠAN ADAMOVIĆ; IBRAHIM MUJIĆ; SLAVICA MILIĆ; ŽIKA LEPOJEVIĆ; ZORAN ZEKOVIĆ

    2009-01-01

    The chemical composition of mentha essential oil and mentha extracts obtained at different pressures/temperatures by supercritical fluid extraction (SFE) were studied by GC–MS. The menthol content was also determined spectrophotometrically. The predominant compounds in the essential oil and in the CO2 extract obtained at 100 bar were L-menthon and menthole but at higher pressures (from 150 to 400 bar), squalene was dominant. The equation of Naik et al. was used for modelling the mentha–superc...

  3. Mentha X dumetorum Schult. aan de Voerenbeek

    NARCIS (Netherlands)

    Jansen, M.T.

    1961-01-01

    Mentha X dumetorum Schult. is de zeldzaam voorkomende bastaard van M. aquatica L. en M. longifolia (L.) Huds. De Voerenbeek of Voer zoeke men niet bij Voerendaal; het is nl. de beek die even bezuiden Eysden, nog juist op Nederlands gebied in de Maas uitmondt. Op Belgisch gebied ontspringt hij bij Si

  4. Southern African species of Mentha L. (Lamiaceae

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    L. E. Codd

    1983-12-01

    Full Text Available The species of Mentha L. occurring in Southern Africa are reviewed and a key is provided to two indigenous and one naturalized species.  M. wissii Launert is reduced to M. longifolia (L. Huds. subsp. wissii (Launert Codd.

  5. Registro de Acromyrmex disciger Mayr, 1887 (Hymenoptera: Formicidae em Cinnamomum zeylanicum (Lauraceae no Município de Braço do Trombudo, Santa Catarina, Brasil

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    Tiago Pikart

    2010-11-01

    Abstract. Cinnamomum zeylanicum Blume (Lauraceae is cultivated for production of leaves, bark and roots, due to its aromatic and spice proprieties. But despite being a resistant plant, C. zeylanicum is subject to attack by various insects and mites during their development, and these pests are responsible for considerable reduction in crop yields. The aim of this study was to record and characterize the attack by leaf-cutting ants in plants of C. zeylanicum in Braço do Trombudo, Santa Catarina State, Brazil between January and March 2010. Damage were characterized by cutting young leaves and shoots of plants of C. zeylanicum with height between 1.0 to 1.5 m and total defoliation of plants less than 1.0 m. The attack in adult plants was not observed. This is the first record of leaf-cutting ant Acromyrmex disciger Mayr damaging plants of C. zeylanicum in Brazil.

  6. Evaluation of metal concentrations in mentha herbal teas (Mentha piperita, Mentha pulegium and Mentha species) by inductively coupled plasma spectrometry.

    Science.gov (United States)

    Rubio, C; Lucas, J R D; Gutiérrez, A J; Glez-Weller, D; Pérez Marrero, B; Caballero, J M; Revert, C; Hardisson, A

    2012-12-01

    Phytopharmaceuticals are gaining popularity worldwide; however, cases of adverse effects and drug interactions have also increased. One reason is in the high metal content both as ingredients but also as contaminants. Metal monitoring in food, like herbal teas, provides basic information on safety aspects in regulatory processes as well as nutritional values. In the present work, Cd, Pb, K, Na, Ca, Mg, Al, B, Ba, Co, Cr, Cu, Fe, Mn, Zn, Li, Ni, and Mo were determined by inductively coupled plasma spectrometry (ICPS) in 36 samples of Mentha sp. Mint tea bags and loose leaves were randomly obtained from supermarkets, traditional markets, herbal stores, and pharmacies in Tenerife (Canary Islands, Spain). Metal contents varied significantly, dependent on the stores the products were purchased in and on tea packaging (loose leaves versus tea bags). Pb analyses revealed levels (0.65±0.71mg/kg) below legal limits. The maximum permissible limit for Cd, 0.3mg/kg, set by the WHO for medicinal plants, was exceeded by 19.44% of the samples (0.22±0.13mg/kg), but all values were below the limit given in the European Pharmacopoeia for this metal (4mg/kg). We observed high Al (151.24±162.73mg/kg) and Li (5.46±3.94mg/kg) levels. B, Ba, Co, Cr, Cu, Fe, Mn, Ni, Zn, and Mo mean levels were 20.51, 14.15, 0.26, 1.65, 10.65, 406.00, 55.05, 1.72, 33.67, and 0.73mg/kg, respectively. Mean Ca, Mg, K, and Na were detected in concentrations of 10.32, 3.83, 7.23 and 1.17g/kg, respectively. In conclusion, metal exposure through herbal mint teas does not seem to be of health concern, as to most of the studied metals, but regulatory limits for Al contents should be imposed.

  7. Constituintes voláteis das folhas e dos galhos de Cinnamomum zeylanicum Blume (Lauraceae Volatile constituents from leaves and branches of Cinnamomum zeylanicum Blume (Lauraceae

    Directory of Open Access Journals (Sweden)

    Maria da Paz Lima

    2005-09-01

    Full Text Available Os óleos essenciais das folhas e dos galhos de Cinnamomum zeylanicum coletada no município de Manaus, estado do Amazonas, foram obtidos por hidrodestilação e analisados através de CG-EM. Vinte e três constituintes foram identificados nas folhas, dos quais o eugenol foi o que apresentou maior porcentagem (60%. Nos galhos foram identificados trinta e seis componentes, com predominância dos monoterpenos alfa- e beta-pineno (9,9%; 3,5%, alfa-felandreno (9,2%, p-cimeno (6,2%, limoneno (7,9%, linalol (10,6%; os sesquiterpenos alfa-copaeno (3,3%, (beta -cariofileno (6,7%, óxido de cariofileno (3,1% e os alilbenzenos (E-cinamaldeído (7,8% e acetato de (E-cinamila (9,7%.The essential oils of the leaves and branches "of Cinnamomum zeylanicum, collected at Manaus, state of Amazonas, were obtained by hydrodistillation and analyzed by GC-MS. Twenty-three components were identified, of which eugenol, was the major (60%. Thirty-six components were identified in the branch oil, among them the monoterpenes alpha-pinene (9.9 %, beta-pinene (3.5 %, alpha phellandrene (9.2 %, p-cymene (6.2%, limonene (7.9%, linalool (10.6% were the most abundant, followed by the sesquiterpenes alpha-copaene (3.3%, (beta-caryophyllene (6.7%, caryophyllene oxide (3.1% and the allybenzenes (E-cinnamaldehyde (7.8%, (E-cinnamyl acetate (9.7%.

  8. Blood chemistry changes in broiler chickens following supplementation with Cinnamomum zeylanicum

    OpenAIRE

    Faixová Zita; Faix Štefan

    2008-01-01

    A study was conducted to investigate the effects of different doses of Cinnamomum zeylanicum in diet on blood biochemistry of broiler chickens. Thirty two, 1-d old male broiler chickens of a commercial strain ROSS 308 were distributed into groups of 8 birds in each one. The chicks received the diets from the day of hatching to 38 d of age. The four types of diets included basal diets for chicks (HYD 01, HYD 02 and HYD 03) supplemented by 0%, 0.1%, 0.05 and 0.025% cinnamon (Cinnamomi aetherole...

  9. Morfologija polenovih zrna roda Mentha L. (Lamiaceae)

    OpenAIRE

    Jančić, Radiša; Polić, Đoka

    1989-01-01

    Utvrđeno je da su polenova zrna vrsta Mentha arven- sis L., M. aquatica L., M. 'longifolia (L.) Huđs., M. spicata L. i M. pulegium L. istog tipa: meridionalno šestobrazdna, prolatna, dugoosa, eliptična. Ekzina je s mrežastom skulpturom. Polenova zrna biljaka vrste M. longifolia uočljivo su manjih dimenzija od polenovih zrna biljaka ostalih istraživanih vrsta.

  10. Overview on mentha and thymus polyphenols

    OpenAIRE

    Olívia R. Pereira; Cardoso, Susana M.

    2013-01-01

    Mentha and Thymus are important genera of the Lamiaceae family widely distributed in the entire World and commonly used in traditional medicine. Indeed, many species of the two genera have been credited with a large list of health-benefit effects, including antioxidant, anti-inflammatory, antimicrobial, analgesic, neuroprotective and anticarcinogenic. In turn, these properties have been associated to the polyphenolic composition of the plants. The present review summarizes the phe...

  11. Supercritical CO2 extraction of mentha (Mentha piperita L. at different solvent densities

    Directory of Open Access Journals (Sweden)

    DUŠAN ADAMOVIĆ

    2009-04-01

    Full Text Available The chemical composition of mentha essential oil and mentha extracts obtained at different pressures/temperatures by supercritical fluid extraction (SFE were studied by GC–MS. The menthol content was also determined spectrophotometrically. The predominant compounds in the essential oil and in the CO2 extract obtained at 100 bar were L-menthon and menthole but at higher pressures (from 150 to 400 bar, squalene was dominant. The equation of Naik et al. was used for modelling the mentha–supercritical CO2 system.

  12. Blood chemistry changes in broiler chickens following supplementation with Cinnamomum zeylanicum

    Directory of Open Access Journals (Sweden)

    Faixová Zita

    2008-01-01

    Full Text Available A study was conducted to investigate the effects of different doses of Cinnamomum zeylanicum in diet on blood biochemistry of broiler chickens. Thirty two, 1-d old male broiler chickens of a commercial strain ROSS 308 were distributed into groups of 8 birds in each one. The chicks received the diets from the day of hatching to 38 d of age. The four types of diets included basal diets for chicks (HYD 01, HYD 02 and HYD 03 supplemented by 0%, 0.1%, 0.05 and 0.025% cinnamon (Cinnamomi aetheroleum of Cinnamomum zeylanicum, Calendula a.s., Nová L'ubovña, Slovakia. Continuous lighting and water and feed ad libitum were provided throughout the trial. The addition of cinnamon to the diets caused a significantly lower plasma glucose level and the effects of cinnamon on plasma glucose levels tended to be dose-dependent. Dietary intake of 0.05 and 0.025% cinnamon reduced serum ALT and plasma potassium levels. Ingestion of cinnamon, however, resulted in no significant changes in circulating calcium, albumin, triglycerides, free glycerol and cholesterol levels. It was concluded that cinnamon could be used not only for flavor and taste in food preparation but it had an additional role in glucose metabolism in broiler chickens.

  13. Anti-Candida activity and chemical composition of Cinnamomum zeylanicum blume essential oil

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    Ricardo Dias de Castro

    2013-10-01

    Full Text Available The purpose of this study was to identify the anti-Candida activity and chemical composition of the essential oil (EO of Cinnamomum zeylanicum (cinnamon. For this, tests were conducted to determine the Minimum Inhibitory Concentration (MIC and Minimum Fungicidal Concentration (MFC and the action of C. zeylanicum EO on fungal cell wall of Candida albicans, C. tropicalis and C. krusei strains. The composition of the was analysed by gas chromatography with mass spectrometry. Significant antifungal activity of the EO was observed on the strains tested, with 87.5% and 62.5% of them sensitive, respectively at a MIC of 312.5 µg/mL and MFC of 2500 µg/mL. In the presence of sorbitol, the MIC was 625 µg/mL against all the strains, showing a possible action of the EO on fungal cell wall. Eugenol (73.27% and trans-β-caryophyllene (5.38% were found in higher concentrations. The results indicated anti-Candida activity of the EO analyzed and suggested that it occurred due to the action on fungal cell wall.

  14. Free Radicals Scavenging Activity of Essential Oils and Different Fractions of Methanol Extract of Zataria Multiflora, Salvia Officinalis, Rosmarinus Officinalis, Mentha Pulegium and Cinnamomum Zeylanicum

    OpenAIRE

    S Changizi Ashtiani; A Malekirad; N Hosseini; M Nazemi

    2012-01-01

    Introduction: Essential oils and extracts from medicinal plants are regarded as natural food preservatives and health promoting drugs. Considering their antioxidant activity, most of them can prevent oxidative stress. The present study was aimed to evaluate free radicals scavenging activity of essential oils and different fractions of methanol extracts from cinnamon, pennyroyal, black cumin, sage, rosemary and azkand. Methods: Antioxidant property of essential oils and different fractions of ...

  15. Antinociceptive activity of Mentha piperita leaf aqueous extract in mice

    OpenAIRE

    Taher, Yousef A.

    2012-01-01

    Background: Mentha piperita L. (Labiatae) is an herbaceous plant, used in folk medicine for the treatment of several medical disorders. Methods and Results: In the present study, the aqueous extract of Mentha piperita leaf, at the i.p doses 200 and 400 mg/kg, showed significant analgesic effects against both acetic acid-induced writhing and hot plateinduced thermal stimulation in mice, with protection values of 51.79% and 20.21% respectively. On the contrary, the Mentha piperita leaf aqueous ...

  16. Photostimulated luminescence detection and radiation effects on cinnamon (Cinnamomum zeylanicum) spice.

    Science.gov (United States)

    Marcazzó, J; Sanchez-Barrera, C E; Urbina-Zavala, A; Cruz-Zaragoza, E

    2015-10-01

    The increase of disease borne pathogens in foods has promoted the use of new technologies in order to eliminate these pathogen microorganisms and extend the shelf-life of the foodstuffs. In particular, Cinnamon (Cinnamomum zeylanicum) contains an important number of pathogen microorganisms and it is frequently sterilized by gamma radiation. However, it is important to develop the detection methods for irradiated food in order to keep the dose control and also to analyze the radiation effects in their chemical property. This work reports (i) the photostimulated luminescence (PSL) detection of irradiated cinnamon and thermoluminescence (TL) detection of the inorganic polymineral fraction separated from this spice, and (ii) the proximate chemical analysis carried out on fat, protein and dietetic fiber contents. The detection limits using the PSL and TL methods were 500 Gy and 10 Gy, respectively, and the fat content was increased significantly with the gamma dose that could be related to the lipid oxidation in the cinnamon. PMID:26133665

  17. Supercritical CO2 extract of Cinnamomum zeylanicum: chemical characterization and antityrosinase activity.

    Science.gov (United States)

    Marongiu, Bruno; Piras, Alessandra; Porcedda, Silvia; Tuveri, Enrica; Sanjust, Enrico; Meli, Massimo; Sollai, Francesca; Zucca, Paolo; Rescigno, Antonio

    2007-11-28

    The volatile oil of the bark of Cinnamomum zeylanicum was extracted by means of supercritical CO2 fluid extraction in different conditions of pressure and temperature. Its chemical composition was characterized by GC-MS analysis. Nineteen compounds, which in the supercritical extract represented >95% of the oil, were identified. (E)-Cinnamaldehyde (77.1%), (E)-beta-caryophyllene (6.0%), alpha-terpineol (4.4%), and eugenol (3.0%) were found to be the major constituents. The SFE oil of cinnamon was screened for its biological activity about the formation of melanin in vitro. The extract showed antityrosinase activity and was able to reduce the formation of insoluble flakes of melanin from tyrosine. The oil also delayed the browning effect in apple homogenate. (E)-Cinnamaldehyde and eugenol were found to be mainly responsible of this inhibition effect. PMID:17966976

  18. Direct enantiomeric analysis of Mentha essential oils.

    Science.gov (United States)

    Barba, Carmen; Santa-María, Guillermo; Herraiz, Marta; Martínez, Rosa M

    2013-11-01

    A rapid and fully automated screening of chiral compounds in essential oils, aimed to the selection of natural sources of pure enantiomers of limonene and carvone, is performed by using on-line coupled reversed phase liquid chromatography with gas chromatography and mass spectrometry (RPLC-GC-MS). Essential oils obtained from Mentha spicata and Mentha piperita were analysed by direct injection into RPLC. The reported procedure includes fractionation and clean-up in RPLC, selection of the fraction to be transferred from RPLC to GC, trapping and concentration of the target compounds in the interface, thermal desorption and, finally, enantiomeric resolution and identification of chiral compounds by GC-MS. The presence of (S)-limonene and (R)-carvone as the unique enantiomeric forms existing for both compounds could be unambiguously established by transferring different volume fractions from RPLC to GC. Data obtained demonstrate high separation efficiency and well tunable selectivity in the on-line coupled RPLC-GC-MS analysis of chiral compounds. PMID:23768391

  19. Direct enantiomeric analysis of Mentha essential oils.

    Science.gov (United States)

    Barba, Carmen; Santa-María, Guillermo; Herraiz, Marta; Martínez, Rosa M

    2013-11-01

    A rapid and fully automated screening of chiral compounds in essential oils, aimed to the selection of natural sources of pure enantiomers of limonene and carvone, is performed by using on-line coupled reversed phase liquid chromatography with gas chromatography and mass spectrometry (RPLC-GC-MS). Essential oils obtained from Mentha spicata and Mentha piperita were analysed by direct injection into RPLC. The reported procedure includes fractionation and clean-up in RPLC, selection of the fraction to be transferred from RPLC to GC, trapping and concentration of the target compounds in the interface, thermal desorption and, finally, enantiomeric resolution and identification of chiral compounds by GC-MS. The presence of (S)-limonene and (R)-carvone as the unique enantiomeric forms existing for both compounds could be unambiguously established by transferring different volume fractions from RPLC to GC. Data obtained demonstrate high separation efficiency and well tunable selectivity in the on-line coupled RPLC-GC-MS analysis of chiral compounds.

  20. Antinociceptive activity of Mentha piperita leaf aqueous extract in mice

    Directory of Open Access Journals (Sweden)

    Yousef A. Taher

    2012-03-01

    Full Text Available Mentha piperita L. (Labiatae is an herbaceous plant, used in folk medicine for the treatment of several medical disorders.In the present study, the aqueous extract of Mentha piperita leaf, at the i.p doses 200 and 400 mg/kg, showed significant analgesic effects against both acetic acid-induced writhing and hot plate-induced thermal stimulation in mice, with protection values of 51.79% and 20.21% respectively. On the contrary, the Mentha piperita leaf aqueous extract did not exhibit anti-inflammatory activity against carrageenan induced paw oedema.These findings indicate that Mentha piperita has a potential analgesic effect that may possibly have mediated centrally and peripherally, as well as providing a pharmacological evidence for its traditional use as a pain reliever.

  1. Southern African species of Mentha L. (Lamiaceae)

    OpenAIRE

    L. E. Codd

    1983-01-01

    The species of Mentha L. occurring in Southern Africa are reviewed and a key is provided to two indigenous and one naturalized species.  M. wissii Launert is reduced to M. longifolia (L.) Huds. subsp. wissii (Launert) Codd.

  2. Mentha L. essential oils composition and in vitro antifungal activity

    OpenAIRE

    Kostik, Vesna; Gjorgjeska, Biljana; Petkovska, Sofija

    2015-01-01

    The essential oils isolated by hydro-distillation from the leaves of wild growing Mentha piperita and Mentha spicata (Lamiaceae) at the region of Mariovo, Republic of Macedonia were analyzed by gas chromatography with flame ionization detector (GC-FID) and gas chromatography wit mass selective detector (GC-MS). A total of forty six and thirty two different components were identified in the essential oils obtained from M. piperita and M. spicata, respectively, constituting approximately >99% (...

  3. Evaluation of Northern Iran Mentha pulegium L. cytotoxicity

    OpenAIRE

    Shirazi, Farshad H.; Neda Ahmadi; Mohammad Kamalinejad

    2004-01-01

    In vitro tests could be a valuable tool for the evaluation of medicinal plants’ cytotoxicity. One of the most frequently used Iranian traditional plants is Mentha Pulegium from Labiatae family. In the present study, essential oil and the methanolic extract of Mentha pulegium, were analyzed for cytotoxicity on human ovary adenocarcinoma SK-OV-3, human malignant cervix carcinoma Hela, and human lung carcinoma A549 cell lines. Two different assays of clonogenic and neutral red (NR) were used for...

  4. IDENTIFICACIÓN DE COMPUESTOS DE MELIA AZEDARACH, SYZGIUM AROMATICUM Y CINNAMOMUM ZEYLANICUM CON EFECTO INHIBITORIO SOBRE BACTERIAS Y HONGOS

    OpenAIRE

    Padrón Márquez, Beatriz; Oranday Cárdenas, Azucena; Rivas Morales, Catalina; Verde Star, María Julia

    2003-01-01

    Se hicieron preparaciones de extractos, pruebas microbiológicas contra ocho microorganismos y caracterización de los compuestos activos de seis extractos obtenidos por dos métodos de extracción, de Melia azedarach, Syzygium aromaticum y Cinnamomum zeylanicum, teniendo como objetivos comparar la eficacia de las técnicas de extracción, y evaluar la actividad antimicrobiana de estas plantas, partiendo del antecedente de que son utilizadas como remedios caseros. Se obse...

  5. CHEMICAL CHARACTERIZATION AND INSECTICIDAL EVALUATION OF THE ESSENTIAL OIL OF Mentha suaveolens L. AND Mentha pulegium L. GROWING IN MOROCCO

    OpenAIRE

    Nisrin Benayad; Weaam Ebrahim; Abdelhak Hakiki; Mahjouba Mosaddak

    2012-01-01

    In this study the chemical composition and insecticidal evaluation of the essential oil of Mentha suaveolens L. and Mentha pulegium L. which are growing in Morocco were investigated. The volatile extract was isolated using hydro-distillation technique followed by continuous liquid-liquid fractionation (Water / Ethyl acetate). The essential oil was then analyzed by gas chromatography (GC) and gas chromatography - mass spectroscopy (GC-MS). The major compounds which are characterized in the ess...

  6. Safety and Tolerability of Essential Oil from Cinnamomum zeylanicum Blume Leaves with Action on Oral Candidosis and Its Effect on the Physical Properties of the Acrylic Resin

    Directory of Open Access Journals (Sweden)

    Julyana de Araújo Oliveira

    2014-01-01

    Full Text Available The anti-Candida activity of essential oil from Cinnamomum zeylanicum Blume, as well as its effect on the roughness and hardness of the acrylic resin used in dental prostheses, was assessed. The safety and tolerability of the test product were assessed through a phase I clinical trial involving users of removable dentures. Minimum inhibitory concentration (MIC and minimum fungicidal concentrations (MFC were determined against twelve Candida strains. Acrylic resin specimens were exposed to artificial saliva (GI, C. zeylanicum (GII, and nystatin (GIII for 15 days. Data were submitted to ANOVA and Tukey posttest (α=5%. For the phase I clinical trial, 15 healthy patients used solution of C. zeylanicum at MIC (15 days, 3 times a day and were submitted to clinical and mycological examinations. C. zeylanicum showed anti-Candida activity, with MIC = 625.0 µg/mL being equivalent to MFC. Nystatin caused greater increase in roughness and decreased the hardness of the material (P<0.0001, with no significant differences between GI and GII. As regards the clinical trial, no adverse clinical signs were observed after intervention. The substance tested had a satisfactory level of safety and tolerability, supporting new advances involving the clinical use of essential oil from C. zeylanicum.

  7. Antioxidant properties of essential oils from Mentha species evidenced by electrochemical methods Propriedades antioxidantes de óleos essenciais de espécies de Mentha evidenciadas por métodos eletroquímicos

    OpenAIRE

    R.S. Gonçalves; Battistin, A; G. Pauletti; Rota, L.; L.A. Serafini

    2009-01-01

    The antioxidant capacity of essential oils of Mentha spicata L., Mentha x gentilis L., Mentha crispa L., Mentha piperita L. and Mentha x piperita L. was determined by using differential pulse voltammetry. This assay was based on the reduction in the limiting current value of the oxygen electroreduction, which was used to calculate the antioxidant capacity (K) of these species. The species M. x gentilis L. had the highest K value. Cyclic voltammetry experiments confirmed the interaction betwee...

  8. Mentha x piperita, Mentha spicata and Effects of Their Essential Oils on Meloidogyne in Soil

    OpenAIRE

    Walker, J. T.; Melin, J. B.

    1996-01-01

    Six peppermint (Mentha x piperita) and six spearmint (M. spicata) PI accessions were inoculated with Meloidogyne incognita race 3 and M. arenaria race 2, under greenhouse conditions. No galls formed on roots of any of the plants inoculated with 1,800 eggs/pot. Fewer than two galls per root system formed on three PI accessions of peppermint inoculated with M. incognita at 5,400 eggs/pot. Only one peppermint accession developed galls when inoculated with M. arenaria, whereas none of the spearmi...

  9. Subcritical water extraction of flavoring and phenolic compounds from cinnamon bark (Cinnamomum zeylanicum).

    Science.gov (United States)

    Khuwijitjaru, Pramote; Sayputikasikorn, Nucha; Samuhasaneetoo, Suched; Penroj, Parinda; Siriwongwilaichat, Prasong; Adachi, Shuji

    2012-01-01

    Cinnamon bark (Cinnamomum zeylanicum) powder was treated with subcritical water at 150 and 200°C in a semi-continuous system at a constant flow rate (3 mL/min) and pressure (6 MPa). Major flavoring compounds, i.e., cinnamaldehyde, cinnamic acid, cinnamyl alcohol and coumarin, were extracted at lower recoveries than the extraction using methanol, suggesting that degradation of these components might occur during the subcritical water treatment. Caffeic, ferulic, p-coumaric, protocatechuic and vanillic acids were identified from the subcritical water treatment. Extraction using subcritical water was more effective to obtain these acids than methanol (50% v/v) in both number of components and recovery, especially at 200°C. Subcritical water treatment at 200°C also resulted in a higher total phenolic content and DPPH radical scavenging activity than the methanol extraction. The DPPH radical scavenging activity and total phenolic content linearly correlated but the results suggested that the extraction at 200°C might result in other products that possessed a free radical scavenging activity other than the phenolic compounds. PMID:22687781

  10. Application of Zataria multiflora Boiss. and Cinnamon zeylanicum essential oils as two natural preservatives in cake

    Science.gov (United States)

    Kordsardouei, Habibe; Barzegar, Mohsen; Sahari, Mohamad Ali

    2013-01-01

    Objective: Oxidation of oils has an important effect on nutritional and organoleptic properties of foodstuffs. Nowadays, new tendency has created a necessity to use natural compounds such as essential oils for producing functional foods. In this study, antioxidant, antifungal, and organoleptic properties of Zataria multiflora Boiss. (ZMEO) and Cinnamon zeylanicum essential oils (CZEO) have been checked as two natural preservatives in the cakes. Materials and Methods: The antioxidant activity of essential oils were determined by measuring thiobarbituric, peroxide, and free fatty acid values of prepared cakes during 60 days storage at 25 ˚C. Antifungal properties of essential oils were determined and given as the ratio of colony number in samples containing ZMEO and CZEO to the control. Results: Different concentrations of essential oils prevented oxidation rate and reducd preliminary and secondary oxidation products compared with butylate hydroxyanisole (BHA (100 and 200 ppm)) and control cakes. Moreover, ZMEO and CZEO at three concentrations (500, 1000, and 1500 ppm) reduced the fungal growth more than samples containing BHA (100 and 200 ppm) and the control. Conclusion: Our results showed that optimum concenteration of ZMEO and CZEO for using in the cakes was 500 ppm therefore it can be replaced instead of synthetic preservatives in foodstuffs. PMID:25050280

  11. Chemical composition of essential oils of wild-growing Mentha piperita L and Mentha spicata L from the Mariovo region, Republic of Macedonia

    OpenAIRE

    Kostik, Vesna; Bauer, Biljana

    2015-01-01

    The genus Mentha (family Lamiaceae), comprising more than 25 species, grows widely throughout the temperate regions of the world. Mentha arvensis, M. longifolia, M. piperita and M. spicata, commonly known as menthol mint, wild mint, peppermint and spearmint, respectively, are frequently cultivated in many countries of Europe, East Asia, America and Australia for the production of essential oils. The essential oils and extracts from Mentha species have been in use since ancient times for the t...

  12. Human Keratinocytes Radioprotection with Mentha Longifolia

    Science.gov (United States)

    Rizzo, Angela Maria; Berselli, P.; Zava, S.; Negroni, M.; Corsetto, P.; Montorfano, G.; Bertolotti, A.; Ranza, E.; Ottolenghi, A.; Berra, B.

    Antioxidants are suggested to act as radioprotectors, and dietary supplements based on antiox-idants have been proposed for astronauts involved in long-term space missions. Plant extracts with antioxidant properties may be used in dietetic supplements for astronauts; in fact recent nutritional guidelines suggest that "fruits and vegetables may become as important on space-going vessels as limes were on the sea-going vessels of old". Mint presents a large variety of biological properties, such as antiallergenic, antibacterial, anti-inflammatory, antitumor, an-tiviral, gastrointestinal protective, hepatoprotective, chemopreventive activities, most of which are attributable to its antioxidant activity. The aim of the present study is to evaluate the antioxidant properties and protective bio-efficacy of a phenol enriched Mentha longifolia ex-tract on gamma rays stressed human keratinocytes (NCTC2544). We assessed first the in vitro antioxidant activity (ABTS and DPPH), and then evaluated different stress markers in order to investigate various oxidative stress targets: cell viability (MTT); retained proliferating ca-pability (CA); DNA damage (histone H2AX) and protein damage (HSP70 induction). Results indicate that this Mint extract has a higher antioxidant activity respect to fresh extracts, that could be responsible of its really interesting radio-protective effects.

  13. Effects of Cinnamomum zeylanicum (Ceylon cinnamon) on blood glucose and lipids in a diabetic and healthy rat model

    OpenAIRE

    Priyanga Ranasinghe; Sanja Perera; Mangala Gunatilake; Eranga Abeywardene; Nuwan Gunapala; Sirimal Premakumara; Kamal Perera; Dilani Lokuhetty; Prasad Katulanda

    2012-01-01

    Objectives: To evaluate short- and long-term effects of Cinnamomum zeylanicum on food consumption, body weight, glycemic control, and lipids in healthy and diabetes-induced rats. Materials and Methods: The study was conducted in two phases (Phase I and Phase II), using Sprague-Dawley rats in four groups. Phase I evaluated acute effects on fasting blood glucose (FBG) (Groups 1 and 2) and on post-oral glucose (Groups 3 and 4) blood glucose. Groups 1 and 3 received distilled-water and Groups 2 a...

  14. The effect of Cinnamomum zeylanicum essential oil on chemical characteristics of Lyoner- type sausage during refrigerated storage.

    Science.gov (United States)

    Aminzare, Majid; Aliakbarlu, Javad; Tajik, Hossein

    2015-01-01

    The effect of Cinnamomum zeylanicum essential oil (CZEO) at two concentrations (0.02% and 0.04% v/w) on chemical composition, pH, water activity (aw), lipid oxidation, color stability and sensory characteristics of Lyoner-type sausage stored at 4 ˚C for 40 days was investigated. The moisture content of the control sample was higher (p 0.05). The water activity content fell in Lyoners with added CZEO during the storage. Incorporation of CZEO retard lipid oxidation process at the end of storage (p sausages.

  15. Antiemetic activity of volatile oil from Mentha spicata and Mentha × piperita in chemotherapy-induced nausea and vomiting

    OpenAIRE

    Tayarani-Najaran, Z; Talasaz-Firoozi, E; Nasiri, R; N Jalali; Hassanzadeh, MK

    2013-01-01

    Background: This study is aimed at determining the efficacy of Mentha spicata (M. spicata) and Mentha × piperita (M. × piperita) in preventing chemotherapy-induced nausea and vomiting (CINV). Methods: This was a randomised, double-blind clinical trial study. Prior to the study, patients were randomly assigned into four groups to receive M. spicata or M. × piperita. Statistical analysis included the χ 2 test, relative risk, and Student’s t-test. Fifty courses were analysed for each group that ...

  16. Evaluation of Northern Iran Mentha pulegium L. cytotoxicity

    Directory of Open Access Journals (Sweden)

    Farshad H. Shirazi

    2004-08-01

    Full Text Available In vitro tests could be a valuable tool for the evaluation of medicinal plants’ cytotoxicity. One of the most frequently used Iranian traditional plants is Mentha Pulegium from Labiatae family. In the present study, essential oil and the methanolic extract of Mentha pulegium, were analyzed for cytotoxicity on human ovary adenocarcinoma SK-OV-3, human malignant cervix carcinoma Hela, and human lung carcinoma A549 cell lines. Two different assays of clonogenic and neutral red (NR were used for evaluation of cytotoxicity. Although the methanolic extract of Mentha pulegium did not show any cytotoxic effects, the essential oil of this plant proved to be a potent cytotoxic agent on the above three cell lines. According to the clonogenic assay, LD50s of the essential oil on SK-OV-3, Hela and A549 cell lines are 14.10, 59.10 and 18.76 µg/ml, respectively. Our findings suggest that Mentha pulegium essential oil might be considered as a potentially toxic agent on human cancer cell lines, and a possible candidate for human cancer chemotherapy. However, further biological tests on the efficacy and side effects of this plant are necessary before its use in human

  17. Evaluation of In-Vitro Antibacterial Acitivity of Cinnamomum zeylanicum Extract on Different Microorganisms of the Dental Plaque

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    Fouad Hussain M. H. Al-Bayaty

    2015-10-01

    Full Text Available The present study was conducted to evaluate the antibacterial activity of aqueous and ethanol extracts of Cinnaomum zeylanicum on different types of dental plaque microorganisms. Screening study was perforned to detect the potential antibacterial activity against S. aureus, E. coli, S. mutans, L. casei, B. fragilis, A. actinomycemtemcomitans and dental plaque pool samples. From the screening test, values of Minimum Inhibitory Concentration (MIC were determined. The lowest MIC value was 25 mg/ml of aqueous and 12.5 mg/ml of ethanol extract for S. aureus. The highest MIC values were seen in A. actinomycetemcomitans and dental plaque aerobic pool samples with 300 mg/ml of aqueous extract and 150 mg/ml of ethanol extract. The MIC values for aqueous extracts ranged from 25 to 300 mg/ml whereas for fixed plant concentration test, showed the strongest inhibition effect for all the organisms tested. Generally, the ethanol extract of Cinnamomum zeylanicum demonstrated a stronger antibacterial activity compared to the aqueous extract. This study also compared the antibacterial activity of chlorhexidine with that of the plant extracts. Chlorhexidine showed a higher antibacterial effect on the microorganisms, with almost all organisms inhibited.

  18. Acute and chronic antihypertensive effects of Cinnamomum zeylanicum stem bark methanol extract in L-NAME-induced hypertensive rats

    Directory of Open Access Journals (Sweden)

    Nyadjeu Paulin

    2013-01-01

    Full Text Available Abstract Background Previous study showed that the aqueous extract of the stem bark of Cinnamomum zeylanicum possesses antihypertensive and vasodilatory properties. The present work investigates the acute and chronic antihypertensive effects of the methanol extract of Cinnamomum zeylanicum stem bark (MECZ in L-NAME-induced hypertensive rats. Methods The acute antihypertensive effects of MECZ (5, 10 and 20 mg/kg administered intravenously were evaluated in rats in which acute arterial hypertension has been induced by intravenous administration of L-NAME (20 mg/kg. For chronic antihypertensive effects, animals were treated with L-NAME (40 mg/kg/day plus the vehicle or L-NAME (40 mg/kg/day in combination with captopril (20 mg/kg/day or MECZ (300 mg/kg/day and compared with control group receiving only distilled water. All drugs were administered per os and at the end of the experiment that lasted for four consecutive weeks, blood pressure was measured by invasive method and blood samples were collected for the determination of the lipid profile. The heart and aorta were collected, weighed and used for both histological analysis and determination of NO tissue content. Results Acute intravenous administration of C. zeylanicum extract (5, 10 and 20 mg/kg to L-NAME-induced hypertensive rats provoked a long-lasting decrease in blood pressure. Mean arterial blood pressure decreased by 12.5%, 26.6% and 30.6% at the doses of 5, 10 and 20 mg/kg, respectively. In chronic administration, MECZ and captopril significantly prevented the increase in blood pressure and organs’ weights, as well as tissue histological damages and were able to reverse the depletion in NO tissue’s concentration. The MECZ also significantly lower the plasma level of triglycerides (38.1%, total cholesterol (32.1% and LDL-cholesterol (75.3% while increasing that of HDL-cholesterol (58.4% with a significant low atherogenic index (1.4 versus 5.3 for L-NAME group. Conclusion MECZ

  19. EFECTO DE LOS REGULADORES DEL CRECIMIENTO EN LA MULTIPLICACIÓN In Vitro DE Mentha piperita Y Mentha citrata

    Directory of Open Access Journals (Sweden)

    Lianette Godoy

    2005-01-01

    Full Text Available El género Mentha agrupa una cierta cantidad de especies de gran importancia como materia prima en diferentes industrias como la perfumería, la cosmética, la licorería y la farmacéutica. En Cuba, el cultivo de las mentas no constituye hasta el momento un renglón comercial de primer orden; sin embargo, sus extractos tienen cierta importancia por sus propiedades medicinales y su utilización en la fabricación de licores. Este trabajo se propuso como objetivo determinar los requerimientos hormonales para la multiplicación in vitro de Mentha citrata y Mentha piperita, como parte de una tecnología para la multiplicación acelerada y la consiguiente obtención de material de propagación de especies de este género. Se ensayaron cinco tratamientos basados en diferentes concentraciones de ácido indolacético y 6-bencilaminopurina, utilizando como control el medio de Murashige y Skoog sin reguladores del crecimiento. Las variables evaluadas fueron: cantidad de entrenudos/brote, cantidad de brotes y cantidad de raíces. No se detectaron diferencias significativas en la variable cantidad de entrenudos/brote para las especies estudiadas. En cambio, se observaron interacciones significativas especie x tratamiento para las variables cantidad de brotes y cantidad de raíces. El empleo de 6-BAP (1.0 mg.L-1 provocó un incremento significativo en ambas variables para Mentha piperita. La producción de brotes y raíces para M. citrata fue independiente de la combinación hormonal empleada. Se recomienda continuar utilizando el medio de Murashige y Skoog sin reguladores del crecimiento para la multiplicación in vitro de Mentha citrata, y emplear el medio basal de Murashige y Skoog suplementado con 6-BAP (1.0 mg.L-1 para la multiplicación in vitro de Mentha piperita.

  20. AN UPDATED OVERVIEW ON PEPPERMINT (MENTHA PIPERITA L.)

    OpenAIRE

    Paul Rita; Datta K. Animesh

    2011-01-01

    Mentha piperita L. (Family: Lamiaceae; Synonym: M. balsamea Wild; commonly known as peppermint) is a natural hybrid (M. aquatica × M. spicata) with immense therapeutic uses (oldest known medicinal plant species, “medicinal plant of the year 2004”, known as “heirba Buena” meaning good herb) apart from possessing other potential uses (as flavoring agent from chewing gum to after dinner mints, in cosmetics and pharmaceutical products). The plant species is a perennial herb, propagating through s...

  1. Antibacterial and antioxidant activities of Mentha piperita L.

    OpenAIRE

    Rajinder Singh; Muftah A. M. Shushni; Asma Belkheir

    2015-01-01

    The antibacterial activity of peppermint oil and different extracts of Mentha piperita against some Gram-positive and Gram-negative bacterial strains was evaluated in the present research work by agar well diffusion method. It was found that the distilled concentrations of essential oil inhibited the growth of microorganisms and the results were comparable with those of antibiotic gentamycin. Essential oils showed a wider spectrum of activity but less strong inhibition as compared to the inve...

  2. Comparison of mentha extracts obtained by different extraction methods

    OpenAIRE

    Milić Slavica; Lepojević Žika; Adamović Dušan; Mujić Ibrahim; Zeković Zoran

    2006-01-01

    The different methods of mentha extraction, such as steam distillation, extraction by methylene chloride (Soxhlet extraction) and supercritical fluid extraction (SFE) by carbon dioxide (CO J were investigated. SFE by CO, was performed at pressure of 100 bar and temperature of40°C. The extraction yield, as well as qualitative and quantitative composition of obtained extracts, determined by GC-MS method, were compared.

  3. New monocyclic monoterpenoid glycoside from Mentha haplocalyx Briq.

    OpenAIRE

    She Gai-Mei; Xu Chao; Liu Bin

    2012-01-01

    Abstract Two new monocyclic monoterpenoid glycosides, rel-(1R,2S,3R,4R) p-menthane-1,2,3-triol 3-O-β-D-glucopyranoside (1) and rel- (1S,2R,3S) terpinolene-1,2,3-triol 3-O-β-D-glucopyranoside (2) were isolated from aqueous acetone extract of the aerial parts of Mentha haplocalyx Briq.. Their structures were elucidated through spectral analysis using MS and NMR spectrometers.

  4. Control of Aspergillus flavus Growth in Tomato Paste by Cinnamomum zeylanicum and Origanum vulgare L. Essential Oils

    Directory of Open Access Journals (Sweden)

    F Kalantary

    2014-05-01

    Full Text Available This study was conducted to evaluate the antifungal activities of cinnamon (Cinnamomum zeylanicum and oregano (Origanum vulgare L. essential oils against Aspergillus flavus in culture media and tomato paste. The chemical compositions of the essential oils were determined by Gas Chromatography-Mass Spectroscopy (GC-MS. Trans- cinnamaldehyde was found to be the main constituent of Cinnamomum zeylanicum essential oil (CZEO, followed by methyl eugenol, δ- cadinene and γ- cadinene. The major components of Origanum vulgare L. essential oil (OVEO were limonene, caryophyllene oxide, α-ionone, germacrene– D, γ- terpinene, β- pinene and terpinene-4-ol. For evaluating antifungal activities of CZEO and OVEO, A. flavus PTCC: 5006, was inoculated in Sabouraud Dextrose Broth (SDB and tomato paste, then 0, 50, 100, 200, 300, 400, 500 and 600 ppm of essential oils were added to each sample and incubated at 25±0.5oC for 30 and 60 days, respectively. The antifungal activity was measured by Agar Dilution method. The EOs at all tested concentrations had inhibitory effect against A. flavus growth. 200 ppm of CZEO and 500 ppm of OVEO completely inhibited A. flavus growth in culture media, while in tomato paste 300 ppm of CZEO and 200 ppm of OVEO had the same effect. Test panel evaluations were carried out in tomato ketchup base and samples with 100 and 200 ppm CZEO were accepted by panelists. The results may suggest the potential replacement of antifungal chemicals by CZEO as natural inhibitor to control A. flavus growth in tomato paste.

  5. The exploitation of micromorphological parameters for identification in the section / Mentha Využitie mikromorfologických parametrov pri identifikácii v sekcii Mentha

    OpenAIRE

    Fialová S.; Halušová L.; Tekeľová D.; Grančai D.

    2015-01-01

    Identifikacia druhov v rode Mentha L. je obzvlašť naročna. Je to sposobene ľahkym križenim podporenym gynodioeciou, ktora je ďalej skomplikovana polymorfizmom, pestovanim, polyploiditou a vegetativnym rozmnožovanim, čo vysvetľuje genetickopopulačne a mikroevolučne pozadie premenlivosti v rode Mentha. V tejto praci sme študovali využitie mikromorfologickych a fytochemickych parametrov pri identifikacii a charakterizacii vybranych druhov rodu Mentha. Na spodnej pokožke listov 20 populacii Menth...

  6. Chemical composition of essential oils of Thymus and Mentha species and their antifungal activities.

    NARCIS (Netherlands)

    Sokovic, M.D.; Vukojevic, J.; Marin, P.D.; Brkic, D.D.; Vajs, V.; Griensven, van L.J.L.D.

    2009-01-01

    The potential antifungal effects of Thymus vulgaris L., Thymus tosevii L., Mentha spicata L., and Mentha piperita L. (Labiatae) essential oils and their components against 17 micromycetal food poisoning, plant, animal and human pathogens are presented. The essential oils were obtained by hydrodestil

  7. Mentha arvensis exhibit better adaptive characters in contrast to Mentha piperita when subjugated to sustained waterlogging stress.

    Science.gov (United States)

    Phukan, Ujjal J; Mishra, Sonal; Timbre, Khilesh; Luqman, Suaib; Shukla, Rakesh Kumar

    2014-05-01

    Waterlogging is becoming a critical threat to plants growing in areas prone to flooding. Some plants adapt various morphological and biochemical alterations which are regulated transcriptionally to cope with the situation. A comparative study of waterlogging response in two different varieties of Mentha namely Mentha piperita and Mentha arvensis was performed. M. arvensis showed better response towards waterlogging in comparison to M. piperita. M. arvensis maintained a healthy posture by utilizing its carbohydrate content; also, it showed a flourished vegetative growth under waterlogged condition. Soluble protein, chlorophyll content, relative water content, and nitric oxide scavenging activity were comparatively more salient in M. arvensis during this hypoxia treatment. Lipid peroxidation was less in M. arvensis. M. arvensis also showed vigorous outgrowth of adventitious roots to assist waterlogging tolerance. To further investigate the possible gene transcripts involved in this response, we did cDNA subtraction of waterlogging treated M. piperita and M. arvensis seedlings. cDNA subtraction has identified thirty seven novel putative Expressed Sequence Tags which were further classified functionally. Functional classification revealed that maximum percentage of proteins belonged to hypothetical proteins followed by proteins involved in biosynthesis. Some of the identified ESTs were further quantified for their induced expression in M. arvensis in comparison to M. piperita through quantitative real-time PCR.

  8. Safety and Tolerability of Essential Oil from Cinnamomum zeylanicum Blume Leaves with Action on Oral Candidosis and Its Effect on the Physical Properties of the Acrylic Resin

    OpenAIRE

    Julyana de Araújo Oliveira; Ingrid Carla Guedes da Silva; Leonardo Antunes Trindade; Edeltrudes de Oliveira Lima; Hugo Lemes Carlo; Alessandro Leite Cavalcanti; Ricardo Dias de Castro

    2014-01-01

    The anti-Candida activity of essential oil from Cinnamomum zeylanicum Blume, as well as its effect on the roughness and hardness of the acrylic resin used in dental prostheses, was assessed. The safety and tolerability of the test product were assessed through a phase I clinical trial involving users of removable dentures. Minimum inhibitory concentration (MIC) and minimum fungicidal concentrations (MFC) were determined against twelve Candida strains. Acrylic resin specimens were exposed to a...

  9. Antifungal activity of the essential oils of wild-growing Mentha piperita L and Mentha spicata L from the Mariovo region, Republic of Macedonia

    OpenAIRE

    Kostik, Vesna; Bauer, Biljana

    2015-01-01

    The antifungal activity of the essential oils isolated by hydro-distillation from the leaves of wild growing Mentha piperita and Mentha spicata (Lamiaceae) at the region of Mariovo, Republic of Macedonia was tested by disc diffusion method and the micro-dilution broth method (MIC) against six plant pathogenic fungi: Alternaria alternate Alternaria solani, Aspergillus flavus, Aspergillus niger, Fusarium solani and Rhizopus solani. The results from the disc diffusion method followed by MIC indi...

  10. Radiation induced useful mutants of Japanese mint (Mentha arvesis L.)

    International Nuclear Information System (INIS)

    With a view to inducing mutations, dormant suckers of Mentha arvensis var. piperascens were exposed to X- and gamma rays. Individual clones raised from these treatments were screened for morphological and biochemical traits. Clones with apparent mutations in the first vegetative generation were propagated through second generation and those breeding true for the mutated character were isolated. In this way several mutants were isolated, some of which were found to be novel with respect to the quality of oil, besides those economically useful. The study shows that induced mutations could be very useful in the breeding of vegetatively propagated plants. (orig.)

  11. New terpenoids from Mentha pulegium L. and their antimicrobial activity.

    Science.gov (United States)

    Ibrahim, Amany K

    2013-04-01

    In addition to one previously reported compound: two new terpenoidal compounds 1α, 6βdimethyl-5β-hydroxy-4β-(prop-1-en-2-yl)-decahydronaphthalen-2-one (1) and 1-(O-β-D-glucopyranosyl)-2,7-dimethyloct-5-en-3-one (2) were isolated from the chloroformic extract of Mentha pulegium L. The structure elucidation of these compounds was based primarily on 1D and 2D-nuclear magnetic resonance analyses. Compound 1 displayed moderate anti-MRSa (IC50 8.5 µg mL(-1)).

  12. Radiation induced useful mutants of Japanese mint (Mentha arvesis L. )

    Energy Technology Data Exchange (ETDEWEB)

    Kak, S.N.; Kaul, B.L.

    1980-01-01

    With a view to inducing mutations, dormant suckers of Mentha arvensis var. piperascens were exposed to X- and gamma rays. Individual clones raised from these treatments were screened for morphological and biochemical traits. Clones with apparent mutations in the first vegetative generation were propagated through second generation and those breeding true for the mutated character were isolated. In this way several mutants were isolated, some of which were found to be novel with respect to the quality of oil, besides those economically useful. The study shows that induced mutations could be very useful in the breeding of vegetatively propagated plants.

  13. Effects of Cinnamomum zeylanicum (Ceylon cinnamon on blood glucose and lipids in a diabetic and healthy rat model

    Directory of Open Access Journals (Sweden)

    Priyanga Ranasinghe

    2012-01-01

    Full Text Available Objectives: To evaluate short- and long-term effects of Cinnamomum zeylanicum on food consumption, body weight, glycemic control, and lipids in healthy and diabetes-induced rats. Materials and Methods: The study was conducted in two phases (Phase I and Phase II, using Sprague-Dawley rats in four groups. Phase I evaluated acute effects on fasting blood glucose (FBG (Groups 1 and 2 and on post-oral glucose (Groups 3 and 4 blood glucose. Groups 1 and 3 received distilled-water and Groups 2 and 4 received cinnamon-extracts. Phase II evaluated effects on food consumption, body weight, blood glucose, and lipids over 1 month. Group A (n = 8, distilled-water and Group B (n = 8, cinnamon-extracts were healthy rats, while Group C (n = 5, distilled-water and Group D (n = 5, cinnamon-extracts were diabetes-induced rats. Serum lipid profile and HbA1c were measured on D-0 and D-30. FBG, 2-h post-prandial blood glucose, body weight, and food consumption were measured on every fifth day. Results: Phase I: There was no significant difference in serial blood glucose values in cinnamon-treated group from time 0 (P > 0.05. Following oral glucose, the cinnamon group demonstrated a faster decline in blood glucose compared to controls (P < 0.05. Phase II: Between D0 and D30, the difference in food consumption was shown only in diabetes-induced rats (P < 0.001. Similarly, the significant difference following cinnamon-extracts in FBG and 2-h post-prandial blood glucose from D0 to D30 was shown only in diabetes-induced rats. In cinnamon-extracts administered groups, total and LDL cholesterol levels were lower on D30 in both healthy and diabetes-induced animals (P < 0.001. Conclusions: C. zeylanicum lowered blood glucose, reduced food intake, and improved lipid parameters in diabetes-induced rats.

  14. EFECTO DE LOS REGULADORES DEL CRECIMIENTO EN LA MULTIPLICACIÓN In Vitro DE Mentha piperita Y Mentha citrata

    OpenAIRE

    Lianette Godoy; E. Héctor; Díaz, B.; Annarella Chea; Torres, A.

    2005-01-01

    El género Mentha agrupa una cierta cantidad de especies de gran importancia como materia prima en diferentes industrias como la perfumería, la cosmética, la licorería y la farmacéutica. En Cuba, el cultivo de las mentas no constituye hasta el momento un renglón comercial de primer orden; sin embargo, sus extractos tienen cierta importancia por sus propiedades medicinales y su utilización en la fabricación de licores. Este trabajo se propuso como objetivo determinar los requerimientos hormonal...

  15. Cinnamomum zeylanicum extract on the radiolabelling of blood constituents and the morphometry of red blood cells: In vitro assay

    International Nuclear Information System (INIS)

    Effects of Cinnamomum zeylanicum (cinnamon) on the labelling of blood constituents with technetium-99 m(99mTc) and on the morphology of red blood cells were studied. Blood samples from Wistar rats were incubated with cinnamon extract for 1hour or with 0.9% NaCl, as control. Labelling of blood constituents with 99mTc was performed. Plasma (P) and blood cells (BC), soluble (SF-P and SF-BC) and insoluble (IF-P and IF-BC) fractions were separated. The radioactivity in each fraction was counted and the percentage of radioactivity incorporated (%ATI) was calculated. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphological analysis of the red blood cells was evaluated. The data showed that the cinnamon extract decreased significantly (p99mTc, and although our results were obtained with animals, precaution is suggested in interpretations of nuclear medicine examinations involving the labelling of blood constituents in patients who are using cinnamon

  16. Cinnamomum zeylanicum extract on the radiolabelling of blood constituents and the morphometry of red blood cells: In vitro assay

    Energy Technology Data Exchange (ETDEWEB)

    Benarroz, M.O. [Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, 4o Andar, Vila Isabel, 20551-030 Rio de Janeiro, RJ (Brazil); Universidade Federal do Rio Grande do Norte, Programa de Pos-Graduacao em Ciencias da Saude, Avenida General Gustavo Cordeiro de Farias, s/n, 59010-180 Natal, RN (Brazil); Fonseca, A.S. [Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, 4o Andar, Vila Isabel, 20551-030 Rio de Janeiro, RJ (Brazil)], E-mail: adenilso@uerj.br; Rocha, G.S. [Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, 4o Andar, Vila Isabel, 20551-030 Rio de Janeiro, RJ (Brazil); Frydman, J.N.G. [Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, 4o Andar, Vila Isabel, 20551-030 Rio de Janeiro, RJ (Brazil); Universidade Federal do Rio Grande do Norte, Programa de Pos-Graduacao em Ciencias da Saude, Avenida General Gustavo Cordeiro de Farias, s/n, 59010-180 Natal, RN (Brazil); Rocha, V.C.; Pereira, M.O. [Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, 4o Andar, Vila Isabel, 20551-030 Rio de Janeiro, RJ (Brazil)] (and others)

    2008-02-15

    Effects of Cinnamomum zeylanicum (cinnamon) on the labelling of blood constituents with technetium-99 m({sup 99m}Tc) and on the morphology of red blood cells were studied. Blood samples from Wistar rats were incubated with cinnamon extract for 1hour or with 0.9% NaCl, as control. Labelling of blood constituents with {sup 99m}Tc was performed. Plasma (P) and blood cells (BC), soluble (SF-P and SF-BC) and insoluble (IF-P and IF-BC) fractions were separated. The radioactivity in each fraction was counted and the percentage of radioactivity incorporated (%ATI) was calculated. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphological analysis of the red blood cells was evaluated. The data showed that the cinnamon extract decreased significantly (p<0.05) the %ATI on BC, IF-P and IF-BC. No modifications were verified on shape of red blood cells. Cinnamon extracts could alter the labelling of blood constituents with {sup 99m}Tc, and although our results were obtained with animals, precaution is suggested in interpretations of nuclear medicine examinations involving the labelling of blood constituents in patients who are using cinnamon.

  17. The Effectiveness of Cinnamomum zeylanicum, Punica granatum Flower and Capsicum annuum Extracts Against Parascaris equorum Infective Larvae

    Directory of Open Access Journals (Sweden)

    Ehsan RAKHSHANDEHROO

    2016-07-01

    Full Text Available Recent investigations have shown that plants with medicinal peculiarities as good alternative to anthelmintics for livestock. In this study, the anthelmintic effects of three medicinal herbs (Cinnamomum zeylanicum, Punica granatum flower and Capsicum annuum were screened in vitro against the infective larvae of Parascaris equorum. The recovered larvae of the parasite were exposed to four concentrations (50, 75, 100 and 125 mg/mL of the extracts and then they examined for the viability at 0, 10, 20, 30 and 40 minutes after the challenge. The results revealed that all the concentrations of each plant extract had anthelmintic effects on P. equorum larvae. Also, the statistics indicated that there were significant interactions between the concentration of the extracts and time of exposure on the number of viable larvae. In addition, C. annuum extract seemed to be a strong potency to kill larvae at all concentrations from the beginning of the experiment. These results confirmed that those herbal extracts possess good antiparasitic effects against infective larvae of P. equorum and thus could be considered in anthelminth treatment strategies.

  18. Antimicrobial potentials of Mentha longifolia by disc diffusion method.

    Science.gov (United States)

    Bakht, Jehan; Shaheen, Salma; Shafi, Mohammad

    2014-07-01

    This study was conducted for the assessment of the antimicrobial activities of different solvents extracted samples from the aerial parts of Mentha longifolia against ten microbial species through the disc diffusion assay using two different concentrations of 1 and 2 mg disc1. All extracts from Mentha longifolia showed different ranges of antimicrobial activities. Butanol and ethyl acetate fractions showed inhibitory activities against all microbial species. Methanol fraction showed inhibitory effects against all the tested microbial species except Salmonella typhi. Salmonella typhi was also not controlled by methanol, petroleum ether and dichloromethane extracted samples. The most susceptible gram positive bacteria was Bacillus atropheus and Bacillus subtilis and were inhibited by all extracts and Staphylococus aureus was least susceptible among gram positive bacteria. Klebsiella pneumoniae was the most susceptible gram negative bacterium and Salmonella typhi was highly resistant among the gram negative bacteria. Erwinia carotovora and Agrobacterium tumefaciene were susceptible to all fractions. All fractions showed antifungal activities against Candida albicans except water extracted samples.

  19. Inhibitory effect of Cinnamomum zeylanicum Blume (Lauraceae essential oil and beta-pinene on the growth of dematiaceous moulds Efeito inibitório do óleo essencial de Cinnamomum zeylanicum Blume (Lauraceae e beta-pineno sobre o crescimento de fungos dematiáceos

    Directory of Open Access Journals (Sweden)

    Ana Carolina Pessoa Moreira

    2007-03-01

    Full Text Available Dematiaceous moulds are pathogen microorganisms able to act as etiological agents of mycoses with different degrees of severity. This study evaluated the effectiveness of Cinnamomum zeylanicum Blume essential oil and beta-pinene in inhibiting the growth of various strains of dematiaceous moulds (Alternaria brassicola, Cladosporium herbarium, C. resinae, C. cladosporioides, Chaetomum globosum, Curvularia sp., Fonsecaea compacta, Piedraia hortae. Antimicrobial assays were led by determining the Minimum Inhibitory Concentration-MIC using the solid medium diffusion procedure and observing the interference of the MIC values on the mould radial mycelial growth along 14 days. MIC values found to C. zeylanicum essential oil oscillated between 63 and 125 µL/mL. beta-pinene showed MIC value of 125 µL/mL for the most mould strains, however C. resinae and C. globosum were resistant to it in all assayed concentrations. MIC values found to C. zeylanicum essential oil and beta-pinene presented intense fungicidal effect noted by a total inhibition of the mycelial growth of C. cladosporioides and F. compacta along 14 days of exposure. These results showed the intense antimould potential of C. zeylanicum essential oil and beta-pinene which could be regarded in a rational use in pharmaceutical formulations used to treat some mycoses, particularly, those caused by dematiaceous moulds.Os fungos dematiáceos são microrganismos patogênicos capazes de agir como agentes etiológicos de micoses com diferentes graus de severidade. Este estudo avaliou a efetividade do óleo essencial de Cinnamomum zeylanicum Blume e beta-pineno em inibir o crescimento de várias cepas de fungos dematiáceos (Alternaria brassicola, Cladosporium herbarium, C. resinae, C. cladosporioides, Chaetomum globosum, Curvularia, Fonsecaea compacta, Piedraia hortae. Os ensaios antimicrobianos foram conduzidos através da determinação da Concentração Inibitória Mínima-CIM utilizando-se da t

  20. The exploitation of micromorphological parameters for identification in the section / Mentha Využitie mikromorfologických parametrov pri identifikácii v sekcii Mentha

    Directory of Open Access Journals (Sweden)

    Fialová S.

    2015-06-01

    Full Text Available Identifikacia druhov v rode Mentha L. je obzvlašť naročna. Je to sposobene ľahkym križenim podporenym gynodioeciou, ktora je ďalej skomplikovana polymorfizmom, pestovanim, polyploiditou a vegetativnym rozmnožovanim, čo vysvetľuje genetickopopulačne a mikroevolučne pozadie premenlivosti v rode Mentha. V tejto praci sme študovali využitie mikromorfologickych a fytochemickych parametrov pri identifikacii a charakterizacii vybranych druhov rodu Mentha. Na spodnej pokožke listov 20 populacii Mentha sme stanovili veľkosť a počet „žliazok typu Lamiaceae“. Silicu sme analyzovali pomocou GC MS. Identifikovali a charakterizovali sme populacie druhov M. × piperita, M. spicata, M. spicata subsp. condensata, M. spicata var. crispa, M. spicata var. citrata, M. × gentilis, M. aquatic, M. arvensis a M. longifolia. Sledovanie veľkosti a počtu „žliazok typu Lamiaceae prispievaju k charakterizacii niektorych druhov Mentha (najma M. longifolia a M. × piperita.

  1. Potential pharmacological and toxicological basis of the essential oil from Mentha spp.

    Directory of Open Access Journals (Sweden)

    IZA TEIXEIRA ALVES PEIXOTO

    2010-06-01

    Full Text Available

    During the past few years, interest in the potential clinical and pharmacological basis of the efficacy and safety of herbal medicines has increased greatly, due to widespread domestic self-medication with these agents. Some authors have analyzed the use of Mentha ssp. in the pharmacological industry. The essential oil from Mentha spp. is used to treat discomfort of the gastrointestinal tract, irritable bowel syndrome, myalgia and neuralgia, as well as oral mucosal inflammation, and also as an expectorant, an antimicrobial and an ingredient in many analgesic creams. The essential oil also contains chemical compounds that are associated with side effects such as nausea, vomiting, allergic reactions, flushing and headaches. Therefore, the purpose of the present review was to examine the literature on the efficacy and safety of the possible clinical and pharmacological uses of the essential oil from Mentha spp. in human beings. Keywords: Mentha spp. Antimicrobial activity. Essential oil.

  2. Research on Yield, Essential Oil, Contents and Components of Mint (Mentha) Species in Different Ecologies

    OpenAIRE

    ÖZGÜVEN, Mensure; Kirici, Saliha

    1999-01-01

    Mint oil demand is met by imports in Turkey. For this reason, this study was conducted to determine yields and essential oil contents and components of different Mentha piperita cultivars ( M. piperita Ucrainica, M. piperita Mitcham , M. piperita Bulgaristan 36, M. piperita Multimentha, M. piperita Prilubskaja-I) and Mentha species ( M. arvensis var. piperascens (L.), M. aquatica, M. spicata ssp. spicata) in Adana and Pozanti ecological conditions over two years. In Adana, they were harvested...

  3. Chemical composition of essential oils of Thymus and Mentha species and their antifungal activities.

    OpenAIRE

    Sokovic,, M.D.; Vukojevic,, J.; Marin, P.D.; Brkic, D.D.; Vajs, V.; Griensven, van, L.J.L.D.

    2009-01-01

    The potential antifungal effects of Thymus vulgaris L., Thymus tosevii L., Mentha spicata L., and Mentha piperita L. (Labiatae) essential oils and their components against 17 micromycetal food poisoning, plant, animal and human pathogens are presented. The essential oils were obtained by hydrodestillation of dried plant material. Their composition was determined by GC-MS. Identification of individual constituents was made by comparison with analytical standards, and by computer matching mass ...

  4. EFFECT OF DIETARY LEVELS OF SPEARMINT (Mentha spicata) ON BROILER CHICKS PERFORMANCE

    OpenAIRE

    E.O. AMASAIB; B.H. ABD ELRAHMAN; Abdelhameed, A A; B.A. ATTA ELMNAN; A.G. MAHALA

    2013-01-01

    This study was conducted to determine the effect of addition different levels of spearmint (Mentha spicata) on broiler chick’s performance. One hundred and twenty eight day old unsexed (Cobb) broiler chicks were used in this experiment. Birds were distributed randomly into 16 pens (8/pen) as replicates, in a complete randomized design. The experimental diets were formulated with four levels of spearmint (Mentha spicata) of 0, 1, 1.5 and 2%. Feed and water were freely accessed. Feed intake, bo...

  5. Artificial Autotetraploidy Induction Possibility of Two Iranian Endemic Mint (Mentha mozaffarianii) Ecotypes

    OpenAIRE

    Askar GHANI; Seyyed Hossein NEAMATI; Azizi, Majid; Saharkhiz, Mohammad Jamal; Farsi, Mohammad

    2014-01-01

    The present study was conducted to polyploidy possibility induction of two Iranian endemic mint (Mentha mozaffarianii) ecotypes. For this purpose, three experiments were done. The first experiment was factorial, based on completely randomized design with three factors and three replications that rhizomes were used for treatment. The first factor including different colchicine concentrations (0, 0.025, 0.05, 0.1 and 0.2% that 0 as control). The second factor including two Mentha ecotypes (Ecot...

  6. A Geminivirus-Satellite Complex is Associated with Leaf Deformity of Mentha (Mint) Plants in Punjab

    OpenAIRE

    Borah, B. K.; Cheema, G. S.; Gill, C. K.; I. Dasgupta

    2010-01-01

    A widespread leaf deformity disease of mentha (mint), accompanied by whiteflies, the vectors of begomoviruses, was observed in Punjab in the last few years. The presence of begomovirus was indicated by DNA dot-blot analysis using the conserved coat protein and replication-associated protein genes of another begomovirus, Sri Lankan cassava mosaic virus (SLCMV). A DNA fragment (2.0 kb), representing a partial genomic DNA of a begomovirus, amplified from the symptomatic mentha leaves was used to...

  7. Effectiveness of Mentha piperita in the Treatment of Infantile Colic: A Crossover Study

    OpenAIRE

    João Guilherme Bezerra Alves; Rita de Cássia Coelho Moraes de Brito; Telma Samila Cavalcanti

    2012-01-01

    Background. Infantile colic is a distressing and common condition for which there is no proven standard treatment. Objective. To compare the efficacy of Mentha piperita with simethicone in treatment for infantile colic. Methods. A double-blind crossover study was performed with 30 infants attending IMIP, Recife, Brazil. They were randomized to use Mentha piperita or simethicone in the treatment of infantile colic during 7 days with each drug. Primary outcomes were mother_s opinion about respo...

  8. Chemical Composition of Essential Oilsof Thymus and Mentha Speciesand Their Antifungal Activities

    OpenAIRE

    Van Griensven, Leo J. L. D.; Vlatka Vajs; Dejan D. Brkić; Petar D. Marin; Jelena Vukojević; Marina D. Soković

    2009-01-01

    The potential antifungal effects of Thymus vulgaris L., Thymus tosevii L., Mentha spicata L., and Mentha piperita L. (Labiatae) essential oils and their components against 17 micromycetal food poisoning, plant, animal and human pathogens are presented. The essential oils were obtained by hydrodestillation of dried plant material. Their composition was determined by GC-MS. Identification of individual constituents was made by comparison with analytical standards, and by computer matching mass ...

  9. Pharmacological and therapeutic effects of Mentha Longifolia L. and its main constituent, menthol

    OpenAIRE

    Peyman Mikaili; Sina Mojaverrostami; Milad Moloudizargari; Shahin Aghajanshakeri

    2013-01-01

    Mentha longifolia (wild mint) is a popular folk remedy. Some parts of this plant have been used in traditional medicine of Iran and other countries. Many studies have shown various pharmacological and therapeutic effects of the plant. Our aim in preparing this study was to review the traditional uses of M. longifolia together with the pharmacological and therapeutic effects of its entire extract and major compounds. Mentha longifolia is an herb with a wide range of pharmacological properties ...

  10. Potential pharmacological and toxicological basis of the essential oil from Mentha spp.

    OpenAIRE

    IZA TEIXEIRA ALVES PEIXOTO; VIVIAN FERNANDES FURLETTI; PAULA CRISTINA ANIBAL; MARTA CRISTINA TEIXEIRA DUARTE; JOSé FRANCISCO HöFLING

    2010-01-01

    During the past few years, interest in the potential clinical and pharmacological basis of the efficacy and safety of herbal medicines has increased greatly, due to widespread domestic self-medication with these agents. Some authors have analyzed the use of Mentha ssp. in the pharmacological industry. The essential oil from Mentha spp. is used to treat discomfort of the gastrointestinal tract, irritable bowel syndrome, myalgia and neuralgia, as well as oral mucosal in...

  11. OCIMUM BASILICUM AND MENTHA PIPERITA ESSENTIAL OILS INFLUENCE THE ANTIMICROBIAL SUSCEPTIBILITY OF STAPHYLOCOCCUS AUREUS STRAINS

    OpenAIRE

    Dan Mihaiescu; Crina Saviuc; Ioana Marinaş; Carmen Mariana Chifiriuc; Alexandru Mihai Grumezescu; Veronica Lazǎr

    2012-01-01

    Essential oils extracted by microwave assisted hydrodistillation technique from Ocinum basilicum and Mentha piperita were characterized by GC-MS. An adapted diffusion method was used in order to assess the potentiator effect of the essential oils on the susceptibility of Staphylococcus aureus clinical and reference strains to some of the currently used antibiotics, i.e. clindamycin, ciprofloxacin, tetracycline, gentamicin, penicillin and erythromycin.. The Ocimum basilicum and Mentha piperit...

  12. Kinetics of Acetylcholinesterase Inhibition by an Aqueous Extract of Mentha longifolia Leaves

    OpenAIRE

    Chandra Shekhar; Suresh Kumar

    2014-01-01

    Cholinesterase inhibitors are the class of compounds which inhibit cholinesterase enzyme. These are used as drugs for symptomatic treatment of Alzheimer’s disease (AD). The present study, evaluate anti-cholinesterase property of an aqueous extract of Mentha longifolia leaves, which is an aromatic plant traditionally used for several medicinal properties. Ellman’s method was used to determine the acetylcholinesterase (AChE) enzyme inhibitory activity of an aqueous extracts of Mentha longifolia...

  13. Assessment of mint (Mentha spp.) species for large-scale production of plantlets by micropropagation

    OpenAIRE

    Hugo Teixeira Gomes; Patrícia Monah Cunha Bartos; Aline Elita Martins; Sharrine Omari Domingues Oliveira; Jonny Everson Scherwinski-Pereira

    2015-01-01

    Species of the genus Mentha produce essential oils which are widely used in pharmaceutical and cosmetic industries. Current study evaluates the potential for in vitro propagation and estimates mass production of plantlets of Mentha species. Nine species (M. piperita, M. suaveolens, M. canadensis, M. longiflora, M. aquatica, M. arvensis, Mentha x gracilis, M. gracilis and M. spicata) were propagated with five successive 30-day subcultures in MS medium supplemented with NAA (0.05 µM) and BAP (4...

  14. In-vitro Antioxidant Activity and GC/MS Studies on the Leaves of Mentha piperita (Lamiaceae) from Morocco

    OpenAIRE

    Elhoussine derwich; Rachida Chabir; Rachid Taouil; Omar Senhaji

    2011-01-01

    The present study was to evaluate the in vitro antioxidant activity and chemical composition of essential oil of Mentha piperita from Morocco. Mentha species from the Lamiaceae family are widely distributed in Morocco and commonly used as herbal tea, flavoring agent and medicinal plant. In this stud y, the essential oils of Mentha piperita collected in the region of Meknes (Morocco) were obtained by hydro-distillation of the leaves and analysed by gas chromatography equipped with flame ionisa...

  15. Initial Study on the Antibacterial Action of Mentha spicata Linn Extract in Vitro

    Institute of Scientific and Technical Information of China (English)

    ZHANG Hong-ying; WANG Xue-bing; HAN Wei-li; CUI Bao-an

    2010-01-01

    [Objective] The research aimed to study the antibacterial action of Mentha spicata Linn extract in vitro.[Method] The cylinder-plate method was used to measure the inhibitory zone size of Mentha spicata Linn extract.The test-tube double dilution method was used to measure the minimal inhibitory concentration(MIC)of Mentha spicata Linn extract on the four kinds of animal pathogenic bacteria,which could determine the antibacterial effect.[Result] The decoction,alcohol extract and volatile oil of Mentha spicata Linn had the different antibacterial effects on the four kinds of animal pathogenic bacteria.Especially the inhibition effect on the pig staphylococcus was comparatively obvious.MIC of Mentha spicata Linn decoction I,alcohol extract III,volatile oil V and volatile oil VI were respectively 31.25,62.5,2.32 and 2.31 mg/ml.[Conclusion] The decoction,alcohol extract and volatile oil of Mentha spicata Linn had the certain antibacterial effect in vitro,and the extracts which were gained by the different extraction methods had the different inhibition effects on the experimental bacteria.

  16. Comparative analysis of antioxidant activities of fourteen mentha essential oils and their components.

    Science.gov (United States)

    Sitzmann, Judith; Habegger, Ruth; Schnitzler, Wilfried H; Grassmann, Johanna

    2014-12-01

    The essential oils of 14 species and hybrids, respectively, of the genus Mentha were examined for their antioxidant capacity in the ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) assay and in a lipid-peroxidation (LPO) assay. The ABTS(.+) -scavenging capacity of pure essential-oil components and mixtures of them was also tested. In both assays, Mentha×dumetorum (classification not fully confirmed), Mentha suaveolens, and Mentha×villosa (classification not fully confirmed) showed the highest antioxidant capacity, which was ascribed to the components germacrene D, piperitone oxide, and piperitenone oxide. The high antioxidant activity in the LPO assay of the two hybrids Mentha×gracilis and, to a lower degree, of Mentha×dalmatica (classification not fully confirmed) was ascribed to their high contents of cis-ocimene and β-caryophyllene. Of the pure components tested (germacrene D, piperitone oxide, and piperitenone oxide were not tested, as not commercially available), only cis-ocimene showed a distinct antioxidant effect, whereas dihydrocarvone and linalool had pro-oxidant effects in the ABTS assay.

  17. Comparative study of kinetics of adsorption of methylene blue from aqueous solutions using cinnamon plant (Cinnamonum zeylanicum) leaf powder and pineapple (Ananas comosus) peel powder

    OpenAIRE

    Satish Dnyandeo Patil; S. Renukdas; N. T. Patel

    2012-01-01

    Batch adsorption of methylene blue (MB) onto Cinnamon plant (Cinnamonum Zeylanicum) leaf powder (CPLP) and Pineapple (Ananas Comosus) peel powder (PPP) was investigated. Different parameters such as initial sorbate concentration, adsorbent dosage, pH, contact time, agitation speed, temperature and particle size. All isotherm models were found to be best fitting with high values of regression coefficient i.e. for Langmuir (R2 = 0.989 to 0.994 for CPLP and 0.993 to 0.995 for PPP), for Freundlic...

  18. Demonstration that menthofuran synthase of mint (Mentha) is a cytochrome P450 monooxygenase: cloning, functional expression, and characterization of the responsible gene

    NARCIS (Netherlands)

    Bertea, C.M.; Schalk, M.; Karp, F.; Maffei, M.; Croteau, R.

    2001-01-01

    ( )-Menthofuran is an undesirable monoterpenoid component of peppermint (Mentha x piperita) essential oil that is derived from the ,-unsaturated ketone ( )-pulegone. Microsomal preparations, from the oil gland secretory cells of a high ( )-menthofuran-producing chemotype of Mentha pulegium, transfor

  19. Estudos de microscopia óptica e de microscopia eletrônica de varredura em folhas de Mentha spicata e de Mentha spicata x suaveolens (Lamiaceae)

    OpenAIRE

    Martins Maria Bernadete Gonçalves

    2002-01-01

    O presente trabalho tem como objetivo realizar um estudo de anatomia foliar por meio de microscopia óptica e de microscopia eletrônica de varredura em Mentha spicata L. e Mentha spicata X suaveolens, caracterizando histologicamente a lâmina foliar. Secções transversais e paradérmicas da região mediana do limbo foliar mostraram a presença de epiderme unisseriada, coberta por uma fina camada de cutícula, apresentando tricomas glandulares do tipo capitado e peltado e não glandulares unisseriados...

  20. Chrysolina herbacea modulates terpenoid biosynthesis of Mentha aquatica L.

    Directory of Open Access Journals (Sweden)

    Simon Atsbaha Zebelo

    Full Text Available Interactions between herbivorous insects and plants storing terpenoids are poorly understood. This study describes the ability of Chrysolina herbacea to use volatiles emitted by undamaged Mentha aquatica plants as attractants and the plant's response to herbivory, which involves the production of deterrent molecules. Emitted plant volatiles were analyzed by GC-MS. The insect's response to plant volatiles was tested by Y-tube olfactometer bioassays. Total RNA was extracted from control plants, mechanically damaged leaves, and leaves damaged by herbivores. The terpenoid quantitative gene expressions (qPCR were then assayed. Upon herbivory, M. aquatica synthesizes and emits (+-menthofuran, which acts as a deterrent to C. herbacea. Herbivory was found to up-regulate the expression of genes involved in terpenoid biosynthesis. The increased emission of (+-menthofuran was correlated with the upregulation of (+-menthofuran synthase.

  1. AN UPDATED OVERVIEW ON PEPPERMINT (MENTHA PIPERITA L.

    Directory of Open Access Journals (Sweden)

    Paul Rita

    2011-08-01

    Full Text Available Mentha piperita L. (Family: Lamiaceae; Synonym: M. balsamea Wild; commonly known as peppermint is a natural hybrid (M. aquatica × M. spicata with immense therapeutic uses (oldest known medicinal plant species, “medicinal plant of the year 2004”, known as “heirba Buena” meaning good herb apart from possessing other potential uses (as flavoring agent from chewing gum to after dinner mints, in cosmetics and pharmaceutical products. The plant species is a perennial herb, propagating through stolons and yield peppermint oil (extremely used of all the volatile oils of commerce (used parts: leaf, whole plant. Considering the essentiality of M. piperita an overview is conducted involving nearly all aspects to provide unabridged repository of references to present and future researchers for effective exploration of the species in human health benefits and commerce.

  2. Induced mutations in the improvement of mentha species

    International Nuclear Information System (INIS)

    A comprehensive study on the induced mutagenesis of the three commercially important species of Mentha; M. arvensis, var. piperascens; M. citrata and M. piperita, was initiated in 1970. Dormant suckers/stolons were exposed to X- and gamma rays (3 to 10 kR). Treated suckers/stolons were planted singly and surviving seedlings carefully examined for mutations using visual, analytical techniques and olfactory assessment of the oils to detect biochemical and qualitative mutants. The mutants selected were propagated through two vegetative cycles to ascertain their genetic stability and field performance. Eventually, a few economically useful and some novel biochemical mutants were isolated. Desirable mutants were subjected to agronomic and chemical evaluation over a period of three years. Consequently one clone of M. arvensis (RRL-118/3) was released for commercial cultivation early this year. This mutant has a potentiality of giving upto 40% more menthol per unit area over the currently available commercial strain. (author)

  3. Inhibitory activity of Indian spice plant Cinnamomum zeylanicum extracts against Alternaria solani and Curvularia lunata, the pathogenic dematiaceous moulds

    Directory of Open Access Journals (Sweden)

    Sharma Bechan

    2009-03-01

    Full Text Available Abstract Background Dematiaceous moulds are pathogenic microorganisms and act as etiological agents of mycoses with different degrees of severity in humans and animals. These moulds also cause loss of food crops and storage food products. The information regarding antimicrobial efficacy of the plant preparations on these moulds is scanty. The present study reveals phytochemical characterization and the effect of bark and leaf extracts of Indian spice plant, Cinnamomum zeylanicum (Cz, against the growth of two species of dematiaceous moulds, Alternaria solani and Curvularia lunata. Methods Cz bark and leaf samples were sequentially extracted in different solvents using Soxhlet apparatus. Phytochemical analyses of extracts were done as per standard protocols. The antifungal bioassay of extracts was done by hanging drop technique. The inhibition of fungal spore germination was monitored under influence of three different concentrations of extracts. Results The lowest test concentration (50 μg/ml of extracts of Cz bark prepared into acetone and that of Cz leaf into petroleum ether and ethanol exhibited complete inhibition (100% of spore germination in both the moulds. At 100 μg/ml concentration all the extracts showed about 50 to 100% inhibition. However, the treatment of the spores of the two fungal species with highest concentration (500 μg/ml of bark and leaf extracts in all the solvents showed 100% fungicidal activity as it completely arrested the germination of spores. Relatively lower activity of aqueous extracts at 50 and 100 μg/ml concentrations suggests that the antifungal ingredients present in Cz bark and leaf are more soluble in organic solvents than water. Conclusion The results demonstrated that the Cz bark and leaves contain certain fungicidal constituents exhibiting potential antimould activity against A. solani and C. lunata.

  4. Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae)

    OpenAIRE

    Brahmi, Fatiha; Hauchard, Didier; Guendouze, Naima; Madani, Khodir; Kiendrebeogo, Martin; Kamagaju, Léocadie; Stévigny, Caroline; Chibane, Mohamed; Duez, Pierre

    2015-01-01

    International audience The leaves of three Mentha species harvested in Algeria, Mentha spicata L. (MS), Mentha pulegium L. (MP) and Mentha rotundifolia (L.) Huds (MR) were examined for their content in polyphenols and for some activities-linked biological properties these could impart. The contents in total phenolics (TPC) and flavonoids (TFC) were evaluated by the Folin–Ciocalteu and the aluminum chloride methods, respectively. Whereas MS showed the highest TPC (12.0 ± 0.3 mg gallic acid ...

  5. Antioxidant properties of essential oils from Mentha species evidenced by electrochemical methods Propriedades antioxidantes de óleos essenciais de espécies de Mentha evidenciadas por métodos eletroquímicos

    Directory of Open Access Journals (Sweden)

    R.S. Gonçalves

    2009-01-01

    Full Text Available The antioxidant capacity of essential oils of Mentha spicata L., Mentha x gentilis L., Mentha crispa L., Mentha piperita L. and Mentha x piperita L. was determined by using differential pulse voltammetry. This assay was based on the reduction in the limiting current value of the oxygen electroreduction, which was used to calculate the antioxidant capacity (K of these species. The species M. x gentilis L. had the highest K value. Cyclic voltammetry experiments confirmed the interaction between the electrode surface and the active compounds present in the essential oils. A simple electrochemical method for determining the ability of active compounds to chelate Fe (II is proposed.Neste trabalho foram determinadas as capacidades antioxidantes dos óleos extraídos de Mentha spicata L., Mentha x gentilis L., Mentha crispa L., Mentha piperita L. e Mentha x piperita L. pelo método da voltametria de pulso diferencial. Esta determinação baseou-se na diminuição do valor limite da corrente de eletrorredução do oxigênio, a partir do qual foi possível calcular o valor da capacidade antioxidante (K destas espécies. A espécie que apresentou o maior valor de K foi a M. x gentilis L. Os dados experimentais obtidos por voltametria cíclica indicaram a interação entre a superfície do eletrodo de platina e os componentes ativos presentes nos óleos essenciais. Uma metodologia simples para a determinação da habilidade quelante dos componentes ativos dos óleos essenciais em relação ao Fe (II também é apresentada.

  6. Identification of EST-SSRs and molecular diversity analysis in Mentha piperita

    Institute of Scientific and Technical Information of China (English)

    Birendra Kumar; Umesh Kumar; Hemant Kumar Yadav

    2015-01-01

    EST sequences of Mentha piperita available in the public domain (NCBI) were exploited to develop SSR markers. A total of 1316 ESTs were assembled into 155 contigs and 653 singletons and of these, 110 sequences were found to contain 130 SSRs, with a frequency of 1 SSR/3.4 kb. Dinucleotide repeat SSRs were most frequent (72.3%) with the AG/CT (43.8%) repeat motif followed by AT/AT (16.2%). Primers were successfully designed for 68 SSR-containing sequences (62.0%). The 68 primers amplified 13 accessions of M. piperita and 54 produced clear amplicons of the expected size. Of these 54, 33 (61%) were found to be polymorphic among M. piperita accessions, showing from 2 to 4 alleles with an average of 2.33 alleles/SSR, and the polymorphic information content (PIC) value varied between 0.13 and 0.51 (average 0.25). All the amplified SSRs showed transferability among four different species of Mentha, with a highest in Mentha arvensis (87.0%) and minimum in Mentha citrata (37.0%). The newly developed SSRs markers were found to be useful for diversity analysis, as they successfully differentiated among species and accessions of Mentha.

  7. Identification of EST–SSRs and molecular diversity analysis in Mentha piperita

    Directory of Open Access Journals (Sweden)

    Birendra Kumar

    2015-08-01

    Full Text Available EST sequences of Mentha piperita available in the public domain (NCBI were exploited to develop SSR markers. A total of 1316 ESTs were assembled into 155 contigs and 653 singletons and of these, 110 sequences were found to contain 130 SSRs, with a frequency of 1 SSR/3.4 kb. Dinucleotide repeat SSRs were most frequent (72.3% with the AG/CT (43.8% repeat motif followed by AT/AT (16.2%. Primers were successfully designed for 68 SSR-containing sequences (62.0%. The 68 primers amplified 13 accessions of M. piperita and 54 produced clear amplicons of the expected size. Of these 54, 33 (61% were found to be polymorphic among M. piperita accessions, showing from 2 to 4 alleles with an average of 2.33 alleles/SSR, and the polymorphic information content (PIC value varied between 0.13 and 0.51 (average 0.25. All the amplified SSRs showed transferability among four different species of Mentha, with a highest in Mentha arvensis (87.0% and minimum in Mentha citrata (37.0%. The newly developed SSRs markers were found to be useful for diversity analysis, as they successfully differentiated among species and accessions of Mentha.

  8. Identification of EST–SSRs and molecular diversity analysis in Mentha piperita

    Institute of Scientific and Technical Information of China (English)

    Birendra; Kumar; Umesh; Kumar; Hemant; Kumar; Yadav

    2015-01-01

    EST sequences of Mentha piperita available in the public domain(NCBI) were exploited to develop SSR markers. A total of 1316 ESTs were assembled into 155 contigs and 653 singletons and of these, 110 sequences were found to contain 130 SSRs, with a frequency of1 SSR/3.4 kb. Dinucleotide repeat SSRs were most frequent(72.3%) with the AG/CT(43.8%)repeat motif followed by AT/AT(16.2%). Primers were successfully designed for 68SSR-containing sequences(62.0%). The 68 primers amplified 13 accessions of M. piperita and 54 produced clear amplicons of the expected size. Of these 54, 33(61%) were found to be polymorphic among M. piperita accessions, showing from 2 to 4 alleles with an average of2.33 alleles/SSR, and the polymorphic information content(PIC) value varied between 0.13 and 0.51(average 0.25). All the amplified SSRs showed transferability among four different species of Mentha, with a highest in Mentha arvensis(87.0%) and minimum in Mentha citrata(37.0%). The newly developed SSRs markers were found to be useful for diversity analysis, as they successfully differentiated among species and accessions of Mentha.

  9. Mentha piperita effects on wistar rats plasma lipids

    Directory of Open Access Journals (Sweden)

    Sandra Maria Barbalho

    2009-10-01

    Full Text Available The aim of this study was to demonstrate the effects of Mentha piperita juice and tea on plasma lipids of Wistar rats. The animals were divided in control group (CG and four treated groups: TG1 treated with tea and TG2 treated with juice (both receiving commercial rat chow; TG3 and TG4 were fed with supplemented fat chow (with hydrogenated fat and soybean oil and treated respectively with tea and juice. Both tea and juice were administered by intra-gastric route (gavage two times a day for 30 days. TG2 and TG4 animals showed significant decrease in triacylglycerols and increase in HDL-c levels. TG1 and TG2 also showed lower cholesterol values. The levels of LDL-c increased in CG but decreased in the treated groups. Animals of TG1, TG2 and TG4 showed substantial reduction in food intake and in percentage of weight gain. TG3 increased food intake but did not increase the percentage of weight gain.Os efeitos hipolipidêmicos de algumas plantas medicinais já foram demonstrados, mas muitas plantas que são comumente utilizadas pela população precisam ser estudadas. O objetivo deste trabalho foi verificar o efeito da Mentha piperita no perfil lipídico de ratos machos Wistar. Os animais foram divididos em grupo controle (CG e quatro grupos experimentais: TG1 e TG2 receberam ração comercial normal, sendo que TG1 foi tratado com chá e TG2 com suco de M. piperita; TG3 e TG4 receberam ração enriquecida (com gordura hidrogenada e óleo de soja, sendo que TG3 foi tratado com chá e TG4 com suco. O tratamento foi feito por via intragástrica duas vezes ao dia durante 30 dias. Os animais de TG2 e TG4 tiveram diminuição significativa nos níveis de triacilglicerídeos e aumento nos níveis de HDL-c. Houve aumento nos níveis de LDL-c em CG, mas nos demais grupos houve diminuição. TG1, TG2 e TG4 tiveram redução significativa no consumo alimentar e na porcentagem de ganho de peso. TG3 aumentou o consumo alimentar, mas não aumentou a porcentagem de

  10. Inhibitory effect of the essential oil from Cinnamomum zeylanicum Blume leaves on some food-related bacteria Efeito inibitório do óleo essencial das folhas de Cinnamomum zeylanicum Blume sobre bactérias de interesse em alimentos

    Directory of Open Access Journals (Sweden)

    Vinicius Nogueira Trajano

    2010-09-01

    Full Text Available Cinnamomum zeylanicum Blume, Lauraceae, has long been known for having many biological properties. This study aimed to identify the constituents of the essential oil from C. zeylanicum leaves using GC-MS and to assess its inhibitory effect on Salmonella enterica, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa based on MIC and MBC determination and kill-time study. Eugenol (73.27% was the most prevalent compound in the essential oil followed by trans-β-cariophyllene (5.38%, linalool (3.31%, and alcohol cinamic acetate (2.53%. The results showed an interesting antibacterial activity of the oil with MIC ranging from 1.25 to 10 µL.mL-1. MBC values were in the range of 20 - 80 µL.mL-1. A concentration of 10 and 40 µL.mL-1 of the essential oil caused a fast and steady decrease in viable cell count (2 to 5 log cycles of all assayed strains along 24 hours. A concentration of 40 µL.mL-1 of the oil provided a total elimination of the initial inocula of S. aureus after 2 hours. These results show the possibility of regarding the essential oil from C. zeylanicum leaves as alternative sources of antimicrobial compounds to be applied in food conservation systems.Cinnamomum zeylanicum Blume, Lauraceae, é uma espécie vegetal reconhecida, a longo tempo, como possuidora de muitas propriedades biológicas. Este estudo objetivou identificar os constituintes do óleo essencial das folhas de C. zeylanicum, utilizando CG-EM, e avaliar seu efeito inibitório sobre Salmonella enterica, Escherichia coli, Staphylococcus aureus e Pseudomonas aeruginosa através da determinação da CIM e CBM, bem como através da análise do tempo de morte bacteriana. Eugenol (73,27% foi o composto mais prevalente no óleo essencial, seguido por trans-β-cariofileno (5,38%, linalol (3,31% e álcool acetato cinâmico (2,53%. Os resultados mostraram uma interessante atividade antibacteriana do óleo essencial com CIM, variando de 1,25 a 10 µL.mL-1. Os valores de

  11. Effect of cinnamomum zeylanicum blume essential oil on the rowth and morphogenesis of some potentially pathogenic Aspergillus species Efeito do óleo essencial de Cinnamomum zeylanicum Blume sobre o crescimento e morfogênese de algumas espécies de Aspergillus potencialmente patogênicas

    Directory of Open Access Journals (Sweden)

    Egberto Santos Carmo

    2008-03-01

    Full Text Available Cinnamomum zeylanicum Blume is known for a wide range of medicinal properties. This study aimed to assess the interference of C. zeylanicum essential oil on the growth and morphogenesis of some potentially pathogenic Aspergillus species. The essential oil presented strong antifungal effect causing the growth inhibition of the assayed strains and development of large growth inhibition zones. MIC50 and MIC90 values were 40 and 80 µL/mL, respectively. 80, 40 and 20 µL/mL of the oil strongly inhibited the radial mycelial growth of A. niger, A. flavus and A. fumigatus along 14 days. 80 and 40 µL/mL of the oil caused a 100% inhibition of the fungal spore germination. Main morphological changes observed under light microscopy provided by the essential oil in the fungal strains were decreased conidiation, leakage of cytoplasm, loss of pigmentation and disrupted cell structure indicating fungal wall degeneration. It is concluded that C. zeylanicum essential oil could be known as potential antifungal compound, particularly, to protect against the growth of Aspergillus species.Cinnamomum zeylanicum Blume é uma planta conhecida por apresentar ampla variedade de propriedades medicinais. Portanto, este estudo teve por objetivo avaliar a interferência do óleo essencial C. zeylanicum sobre o crescimento e morfogênese de algumas espécies de Aspergillus potencialmente patogênicas. O óleo essencial testado apresentou potente efeito antifúngico demonstrado pela visualização de grandes zonas de inibição de crescimento de todas as linhagens testadas. Os valores de CIM50 e de CIM90 foram 40 e 80 µL/mL, respectivamente. Nas concentrações de 80, 40 e 20 µL/mL o óleo demonstrou um potente efeito fumigante, inibindo o crescimento micelial radial de A. niger, A. flavus e A. fumigatus ao longo de 14 dias de exposição. A 80 e 40 µL/mL o óleo essencial promoveu inibição de 100% da germinação de esporos, das três espécies de Aspergillus citadas

  12. In vitro anticancer activity of extracts of Mentha Spp. against human cancer cells.

    Science.gov (United States)

    Sharma, Vikas; Hussain, Shabir; Gupta, Moni; Saxena, Ajit Kumar

    2014-10-01

    In vitro anticancer potential of methanolic and aqueous extracts of whole plants of Mentha arvensis, M. longifolia, M. spicata and M. viridis at concentration of 100 μg/ml was evaluated against eight human cancer cell lines--A-549, COLO-205, HCT-116, MCF-7, NCI-H322, PC-3, THP-1 and U-87MG from six different origins (breast, colon, glioblastoma, lung, leukemia and prostate) using sulphorhodamine blue (SRB) assay. Methanolic extracts of above-mentioned Mentha Spp. displayed anti-proliferative effect in the range of 70-97% against four human cancer cell lines, namely COLO-205, MCF-7, NCI-H322 and THP-1; however, aqueous extracts were found to be active against HCT-116 and PC-3. The results indicate that Mentha Spp. contain certain constituents with cytotoxic properties which may find use in developing anticancer agents. PMID:25630112

  13. In vitro anticancer activity of extracts of Mentha Spp. against human cancer cells.

    Science.gov (United States)

    Sharma, Vikas; Hussain, Shabir; Gupta, Moni; Saxena, Ajit Kumar

    2014-10-01

    In vitro anticancer potential of methanolic and aqueous extracts of whole plants of Mentha arvensis, M. longifolia, M. spicata and M. viridis at concentration of 100 μg/ml was evaluated against eight human cancer cell lines--A-549, COLO-205, HCT-116, MCF-7, NCI-H322, PC-3, THP-1 and U-87MG from six different origins (breast, colon, glioblastoma, lung, leukemia and prostate) using sulphorhodamine blue (SRB) assay. Methanolic extracts of above-mentioned Mentha Spp. displayed anti-proliferative effect in the range of 70-97% against four human cancer cell lines, namely COLO-205, MCF-7, NCI-H322 and THP-1; however, aqueous extracts were found to be active against HCT-116 and PC-3. The results indicate that Mentha Spp. contain certain constituents with cytotoxic properties which may find use in developing anticancer agents.

  14. Effects of Mentha suaveolens essential oil on Chlamydia trachomatis.

    Science.gov (United States)

    Sessa, Rosa; Di Pietro, Marisa; De Santis, Fiorenzo; Filardo, Simone; Ragno, Rino; Angiolella, Letizia

    2015-01-01

    Chlamydia trachomatis, the most common cause of sexually transmitted bacterial infection worldwide, has a unique biphasic developmental cycle alternating between the infectious elementary body and the replicative reticulate body. C. trachomatis is responsible for severe reproductive complications including pelvic inflammatory disease, ectopic pregnancy, and obstructive infertility. The aim of our study was to evaluate whether Mentha suaveolens essential oil (EOMS) can be considered as a promising candidate for preventing C. trachomatis infection. Specifically, we investigated the in vitro effects of EOMS towards C. trachomatis analysing the different phases of chlamydial developmental cycle. Our results demonstrated that EOMS was effective towards C. trachomatis, whereby it not only inactivated infectious elementary bodies but also inhibited chlamydial replication. Our study also revealed the effectiveness of EOMS, in combination with erythromycin, towards C. trachomatis with a substantial reduction in the minimum effect dose of antibiotic. In conclusion, EOMS treatment may represent a preventative strategy since it may reduce C. trachomatis transmission in the population and, thereby, reduce the number of new chlamydial infections and risk of developing of severe sequelae.

  15. Antioxidant activity of gilan Mentha pulegium during growth.

    Science.gov (United States)

    Shahmohamadi, Rezvan; Sariri, Reyhaneh; Rasa, Mahdi; Aghamali, Mahmudreza

    2014-02-01

    Antioxidant activity and total phenolic contents of methanolic and hydroalcoholic-acetone extracts of Iranian Mentha pulegium in two stages of maturity were investigated. The aim was to investigate the most suitable solvent for extraction of antioxidants and to find the correlation existed between plant growth stage and its antioxidant capacity. In vitro antioxidant properties of the extracts were examined by 1, 1 diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing/Antioxidant Power (FRAP) methods. Moreover, total phenolic contents were determined by the Folin-Ciocalteu method. On the other hand, the phenolic compounds were analyzed by chromatographic methods, TLC and SPE-reversed phase-HPLC. The results revealed that the antioxidant capacities and total phenol contents of the extracts in flowering season were higher than pre-flowering season. A positive relationship was found between the results obtained from three different assay methods used, i.e. FRAP, DPPH and phenol contents. The TLC chromatogram of the two extracts showed differences in the number of separated compounds of extracts. HPLC results indicated that the fraction collected with washing buffer (pH = 6) had highest antioxidant activity.

  16. Antibacterial and antioxidant activities of Mentha piperita L.

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    Rajinder Singh

    2015-05-01

    Full Text Available The antibacterial activity of peppermint oil and different extracts of Mentha piperita against some Gram-positive and Gram-negative bacterial strains was evaluated in the present research work by agar well diffusion method. It was found that the distilled concentrations of essential oil inhibited the growth of microorganisms and the results were comparable with those of antibiotic gentamycin. Essential oils showed a wider spectrum of activity but less strong inhibition as compared to the investigated commercial antibiotic. Minimum inhibitory concentrations (MICs for the bacterial species ranged from 0.4% to 0.7% v/v. The oil and extracts also exhibited significant antioxidant activity and the oil showed about half potency when compared to the standard BHT. These results indicated the strong antibacterial and antioxidant activities of peppermint oil but additional investigations need to be performed in order to confirm the safety of these concentrations (MIC for human consumption. Peppermint oil could be used as a good conservation agent by inhibiting some food borne pathogens.

  17. CONSTITUENTS OF THE ESSENTIAL OIL OF "MENTHA MOZAFFARIANI JAMZAD"

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    DjA.Rustaiyan

    1994-08-01

    Full Text Available The genus Mont ha is widely distributed in Iranian territory and comprises several species reported in folk medicine as having biological activity (1. Mentha mozaffariani Jamzad, growing in the wild in southern Iran, near Bandar-Abass (40 km. north of the Persian Gulf, contains an essential oil, the exact composition of which has not yet, as far as we know, been reported. Spectrometric scanning (IR and H-NMR of the crude oils indicated their terpenic nature."nThe aerial parts and flowers of this plant were investigated by means of gas chromatography, mass spectrometry and high-resolution H-NMR of the main compounds. The composition of the volatile mixtures was determined by comparison with data from the literature, and databases of the retention times and Kovats indices."nThe compounds most frequently found in the oils studied are:-caryophyllene, a - Humulene, calamenene and piperitone epoxide. Among hydroxylated terpenes, a -terpineol, 4-terpineol, linalool and borneol were found.

  18. Effects of Mentha suaveolens Essential Oil on Chlamydia trachomatis

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    Rosa Sessa

    2015-01-01

    Full Text Available Chlamydia trachomatis, the most common cause of sexually transmitted bacterial infection worldwide, has a unique biphasic developmental cycle alternating between the infectious elementary body and the replicative reticulate body. C. trachomatis is responsible for severe reproductive complications including pelvic inflammatory disease, ectopic pregnancy, and obstructive infertility. The aim of our study was to evaluate whether Mentha suaveolens essential oil (EOMS can be considered as a promising candidate for preventing C. trachomatis infection. Specifically, we investigated the in vitro effects of EOMS towards C. trachomatis analysing the different phases of chlamydial developmental cycle. Our results demonstrated that EOMS was effective towards C. trachomatis, whereby it not only inactivated infectious elementary bodies but also inhibited chlamydial replication. Our study also revealed the effectiveness of EOMS, in combination with erythromycin, towards C. trachomatis with a substantial reduction in the minimum effect dose of antibiotic. In conclusion, EOMS treatment may represent a preventative strategy since it may reduce C. trachomatis transmission in the population and, thereby, reduce the number of new chlamydial infections and risk of developing of severe sequelae.

  19. Positive correlation between menthol content and in vitro menthol tolerance in Mentha arvensis L. cultivars

    Indian Academy of Sciences (India)

    Ajit K Shasany; Suman P S Khanuja; Sunita Dhawan; Sushil Kumar

    2000-09-01

    Menthol is a highly valued monoterpene produced by Japanese mint (Mentha arvensis) as a natural product with wide applications in cosmetics, confectionery, flavours, beverages and therapeutics. Selection of high menthol yielding genotypes is therefore the ultimate objective of all genetic improvement programmes in Mentha arvensis. A positive correlation was observed in the present study between menthol content in oils of evaluated genotypes and the level of tolerance to externally supplied menthol of explants of these genotypes in culture medium. The easy use of this relationship as a selectable biochemical marker opens the practical applicability of large-scale in vitro screening of the germplasm, clones and breeders’ material for selection of elite genotypes.

  20. Estudos de microscopia óptica e de microscopia eletrônica de varredura em folhas de Mentha spicata e de Mentha spicata x suaveolens (Lamiaceae) Optical microscopy studies and scanning electron microscopy in leaf the Mentha spicata and Mentha spicata x suaveolens (Lamiaceae)

    OpenAIRE

    Maria Bernadete Gonçalves Martins

    2002-01-01

    O presente trabalho tem como objetivo realizar um estudo de anatomia foliar por meio de microscopia óptica e de microscopia eletrônica de varredura em Mentha spicata L. e Mentha spicata X suaveolens, caracterizando histologicamente a lâmina foliar. Secções transversais e paradérmicas da região mediana do limbo foliar mostraram a presença de epiderme unisseriada, coberta por uma fina camada de cutícula, apresentando tricomas glandulares do tipo capitado e peltado e não glandulares unisseriados...

  1. PHENOLIC COMPOUNDS OF WATER-ETHANOLIC EXTRACT OF MENTHA LONGIFOLIA L

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    O. A. Grebennikova

    2014-01-01

    Full Text Available The article represents data about qualitative and quantitative composition of phenolic compounds in water-ethanol extract of perspective clone of Mentha longifolia L. of NBE-NSC selection. Phenolic substances content in water-ethanol extract amounted to 3003.3 mg/100g. 13 components were determined in the extract. The extract contains caffeic acid, chlorogenic acid isomers, rosmarinic acid and glycosides of luteolin. Rosmarinic acid (50.2% prevails among phenolic substances of Mentha longifolia extract. The conclusion is that the use of this extract is possible to create products with high biological value

  2. OCIMUM BASILICUM AND MENTHA PIPERITA ESSENTIAL OILS INFLUENCE THE ANTIMICROBIAL SUSCEPTIBILITY OF STAPHYLOCOCCUS AUREUS STRAINS

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    Dan Mihaiescu

    2012-03-01

    Full Text Available Essential oils extracted by microwave assisted hydrodistillation technique from Ocinum basilicum and Mentha piperita were characterized by GC-MS. An adapted diffusion method was used in order to assess the potentiator effect of the essential oils on the susceptibility of Staphylococcus aureus clinical and reference strains to some of the currently used antibiotics, i.e. clindamycin, ciprofloxacin, tetracycline, gentamicin, penicillin and erythromycin.. The Ocimum basilicum and Mentha piperita essential oils, exhibited a strong, strain specific influence on the antibiotic susceptibility of the tested strains.

  3. PHENOLIC COMPOUNDS OF WATER-ETHANOLIC EXTRACT OF MENTHA LONGIFOLIA L

    OpenAIRE

    O. A. Grebennikova; A. E. Paliy; V. D. Rabotyagov

    2014-01-01

    The article represents data about qualitative and quantitative composition of phenolic compounds in water-ethanol extract of perspective clone of Mentha longifolia L. of NBE-NSC selection. Phenolic substances content in water-ethanol extract amounted to 3003.3 mg/100g. 13 components were determined in the extract. The extract contains caffeic acid, chlorogenic acid isomers, rosmarinic acid and glycosides of luteolin. Rosmarinic acid (50.2%) prevails among phenolic substances of Mentha longifo...

  4. Efficiency of Mentha piperita L. and Mentha pulegium L. essential oils on nutritional indices of Plodia interpunctella Hübner (Lepidoptera: Pyralidae

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    K. Saeidi

    2014-04-01

    Full Text Available Antifeedant activity of plant extracts from Mentha piperita L. and Mentha pulegium L. were tested against the Indian meal moth, Plodia interpunctella (Hübner. The nutritional indices: relative growth rate (RGR, relative consumption rate (RCR, efficiency of conversion of ingested food (ECI and feeding deterrence index (FDI were measured for first-instar larvae (15-d old. Treatments were evaluated using a flour disk bioassay in the dark, at 25±1°C and 60±5% R.H. Concentrations of 0, 0.1, 0.5, 0.75, 1, 1.5 and 2 mL/disk were prepared from each essential oil. After 72 h, nutritional indices were calculated. M. piperita oils were more effective than M. pulegium oils, by significantly decreasing the RGR, RCR and FDI. At the highest concentration tested (2 mL/disk, the ECI (9% was significantly reduced.

  5. Diurnal effects on Mentha canadensis oil yields and composition at two different harvests

    Science.gov (United States)

    Japanese cornmint, also known as menthol mint, (Mentha canadensis L. syn M. arvensis var canadensis L.), is an essential oil crop cultivated in several countries in Asia and South America. The plant is currently the only commercially viable source for natural menthol due to the high concentration of...

  6. Capillary electrophoresis fingerprinting and spectrophotometric determination of antioxidant potential for classification of Mentha products.

    Science.gov (United States)

    Roblová, Vendula; Bittová, Miroslava; Kubáň, Petr; Kubáň, Vlastimil

    2016-07-01

    In this work aqueous infusions from ten Mentha herbal samples (four different Mentha species and six hybrids of Mentha x piperita) and 20 different peppermint teas were screened by capillary electrophoresis with UV detection. The fingerprint separation was accomplished in a 25 mM borate background electrolyte with 10% methanol at pH 9.3. The total polyphenolic content in the extracts was determined spectrophotometrically at 765 nm by a Folin-Ciocalteu phenol assay. Total antioxidant activity was determined by scavenging of 2,2-diphenyl-1-picrylhydrazyl radical at 515 nm. The peak areas of 12 dominant peaks from CE analysis, present in all samples, and the value of total polyphenolic content and total antioxidant activity obtained by spectrophotometry was combined into a single data matrix and principal component analysis was applied. The obtained principal component analysis model resulted in distinct clusters of Mentha and peppermint tea samples distinguishing the samples according to their potential protective antioxidant effect. Principal component analysis, using a non-targeted approach with no need for compound identification, was found as a new promising tool for the screening of herbal tea products.

  7. Capillary electrophoresis fingerprinting and spectrophotometric determination of antioxidant potential for classification of Mentha products.

    Science.gov (United States)

    Roblová, Vendula; Bittová, Miroslava; Kubáň, Petr; Kubáň, Vlastimil

    2016-07-01

    In this work aqueous infusions from ten Mentha herbal samples (four different Mentha species and six hybrids of Mentha x piperita) and 20 different peppermint teas were screened by capillary electrophoresis with UV detection. The fingerprint separation was accomplished in a 25 mM borate background electrolyte with 10% methanol at pH 9.3. The total polyphenolic content in the extracts was determined spectrophotometrically at 765 nm by a Folin-Ciocalteu phenol assay. Total antioxidant activity was determined by scavenging of 2,2-diphenyl-1-picrylhydrazyl radical at 515 nm. The peak areas of 12 dominant peaks from CE analysis, present in all samples, and the value of total polyphenolic content and total antioxidant activity obtained by spectrophotometry was combined into a single data matrix and principal component analysis was applied. The obtained principal component analysis model resulted in distinct clusters of Mentha and peppermint tea samples distinguishing the samples according to their potential protective antioxidant effect. Principal component analysis, using a non-targeted approach with no need for compound identification, was found as a new promising tool for the screening of herbal tea products. PMID:27306863

  8. [Relativity of commercial specification of Menthae Herba based on chemical analysis].

    Science.gov (United States)

    Ye, Dan; Zhao, Ming; Shao, Yang; Ouyang, Zhen; Peng, Hua-sheng; Han Bang-xing; Zhang, Wei-wan-qi; Gu, Xue-mei

    2015-01-01

    In order to compare the differences of 35 Menthae Herba samples collected on the market and at producing areas, the contents of six total terpenoids, the essential oil and chromatographic fingerprints were analyzed, which provided evidences for drawing up the commodity specifications and grading criteria of Menthae Herba. GC-MS method was used to analyze the chemical constituents of 35 different samples. The chromatographic fingerprints obtained by using GC were then evaluated by similarity analysis, hierarchical clustering analysis and principal component analysis. The relativity between the content of six terpenoids and the essential oil were studied. In this study, the chemical profiles of 35 samples from different producing areas had significant disparity. All samples collected in the report could be categorized into four chemical types, L-menthol, pulegone, carvone and L-menthone, but the chemical profiles had no relationship with the areas. The chromatographic fingerprints of the samples from different types were dissimilar, while the different producing areas were difficult to be separated. It was indicated that the content of volatile oil was positively correlated with the content of L-menthol and the sum of six total terpenoids. The content of the essential oil, L-menthol and the sum of six total terpenoids of Menthae Herba were considered as one of the commercial specifications and grading criteria. These results in the research could be helpful to draw up the commercial specification and grading criteria of Menthae Herba from a view of chemical information.

  9. Enhanced rosmarinic acid production in cultured plants of two species of Mentha.

    Science.gov (United States)

    Roy, Debleena; Mukhopadhyay, Sandip

    2012-11-01

    In the present investigation an attempt has been made to enhance rosmarinic acid level in plants, grown in vitro, of 2 species of Mentha in presence of 2 precursors in the nutrient media during culture. For in vitro culture establishment and shoot bud multiplication, MS basal media were used supplemented with different concentrations and combinations of different growth regulator like NAA (alpha-napthaleneacetic acid), BAP (6-benzylaminopurine). The medium containing NAA (0.25 mg/L) and BAP (2.5 mg/L) gave the highest potentiality of shoot formation (average 58.0 numbers of shoots) per explant for Mentha piperita L. and the medium containing BAP (2.0 mg/L) gave the highest potentiality of shoot (average 19.2 numbers of shoots) formation per explant for Mentha arvensis L. The complete plants were regenerated in above mentioned media after 8 weeks of subculture. For in vitro enhancement of rosmarinic acid production, the 2 precursors tyrosine (Tyr) and phenylalanine (Phe) were added in the nutrient media at different levels (0.5 mg/L to 15.0 mg/L). Tyrosine was found to be very effective for augmenting rosmarinic acid content in Mentha piperita L. It nearly increased the production up to 1.77 times. In case of Mentha arvensis L., phenylalanine significantly affected the production of rosmarinic acid and the production was nearly 2.03 times more than the control. No significant increase in biomass was observed after addition of these precursors indicating that the added amino acids acting as precursors for rosmarinic acid synthesis were readily utilized in producing rosmarinic acid without promoting growth. Total protein profile also revealed the presence of a specific band in polyacrylamide gel electrophoresis.

  10. Evaluation of Mentha Piperita and NaCl effects on Streptococcus mutans

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    M. Raeisi

    2016-01-01

    Full Text Available Introduction & Objective: Dental caries is an infectious disease caused by colonization of Streptococcus mutans bacteria, which is begun by decalcification of non-organic part of teeth and continued with destruction of organic matrix. According to the incidence of relative resistance of biocide and also antibiotics in the normal micro flora of mouth specially viridians species and recent trends in the use of medicinal herbs to prevent and treat diseases due to fewer side effects, in this study, the MBC and MIC effect of hydro alcoholic extract of Mentha Piperita and NaCl on S. mutans in vitro were evaluated. Materials & Methods: In this experimental study hydro alcoholic extract has been prepared from Mentha Piperita with Maceration method (soaking in a solvent. After the preparation of the S. mutans strain standard and sterilization of hydro alcoholic extract by filtering, the antibacterial effects have been evaluated by Agar well diffusion, Agar disk diffusion and broth micro dilution methods (Concentration in the range of 0.78 - 800 micrograms per milliliter (µg/ml. Meanwhile, the concentration range of sodium chloride 0.5 - 10 % was prepared and its effect on the test bacteria was determined. Result: In Agar well diffusion method and Agar disk diffusion method the hydro alcoholic extract of Mentha Piperita and NaCl had no antibacterial effect on S. mutans. In broth micro dilution method, hydro alcoholic extract of Mentha Piperita had no antibacterial effect on S. mutans but the MIC and MBC of NaCl was 5% and 5.5% , respectively. Conclusion: Our results indicated that the hydro alcoholic extract of Mentha Piperita had not any antibacterial property against S. mutans but the most effective antibacterial concentration of NaCl was 5 - 5.5 percent. Sci J Hamadan Univ Med Sci . 2016; 22 (4 :309-315

  11. Sinergismo entre óleos essenciais e drogas antimicrobianas sobre linhagens de Staphylococcus aureus e Escherichia coli isoladas de casos clínicos humanos Synergism between essential oils and antimicrobial drugs against Staphylooccus aureus and Escherichia coli strains from human infections

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    Juliana A. A. Zago

    2009-12-01

    Full Text Available Estudos com plantas e utilização em terapias combinatórias têm sido estimulados. Verificou-se as possíveis interações entre óleos essenciais de plantas [canela (Cinnamomum zeylanicum Blume Lauraceae, capim-cidreira (Cymbopogon citratus (DC. Stapf, Poaceae, hortelã-pimenta (Mentha piperita L. Lamiaceae, gengibre (Zingiber officinale Roscoe Zingiberaceae, cravo-da-índia (Caryophillus aromaticus L. Myrtaceae e alecrim (Rosmarinus officinalis L. Lamiaceae] combinados a oito drogas antimicrobianas frente a doze linhagens de Staphylococcus aureus e doze de Escherichia coli isoladas de humanos. Após determinação da Concentração Inibitória Mínima (CIM para os óleos pelo método da diluição foram realizados ensaios para verificação de sinergismo entre os óleos essenciais e os antimicrobianos pela metodologia de Kirby & Bauer. S. aureus foi mais suscetível às interações óleos e drogas, tendo o óleo de capim cidreira apresentado sinergismo com as oito drogas testadas, seguido pelo óleo de hortelã com sete drogas. Nos ensaios com E. coli, houve sinergismo apenas para os óleos de alecrim (três drogas e capim-cidreira (duas drogas. Não ocorreram casos de antagonismo e os resultados de sinergismo foram influenciados pelos microrganismos estudados.The studies with plants and combinatory therapy have been stimulated. The interactions between cinnamon (Cinnamomun zeylanicum Blume Lauraceae, lemon grass (Cymbopogon citratus (DC. Stapf, Poaceae, mint (Mentha piperita L. Lamiaceae, ginger (Zingiber officinale Roscoe Zingiberaceae, clove (Caryophillus aromaticus L. Myrtaceae and rosemary (Rosmarinus officinalis L. Lamiaceae and eight antimicrobial drug was carried. It was made against twelve S. aureus and twelve E.coli strains isolated from human specimens. After minimal inhibitory concentration (MIC values determination of essential oils by dilution agar method, the synergism assays were performed by Kirby and Bauer method. The S

  12. ANTIMICROBIAL ACTIVITY OF MENTHA ARVENSIS AGAINST CLINICAL ISOLATES OF HUMAN CARIOGENIC PATHOGENS- AN IN-VITRO STUDY

    OpenAIRE

    Vinod Singh et al.

    2012-01-01

    Patients with chronic dental infection are usually treated with antibiotics. However, the value of antibiotics was decreasing because increased resistance in bacteria. The objective of this study is to evaluate the efficacy of herbal crude extract of Mentha arvensis in human Cariogenic pathogens. In this study we obtained crud extract of Mentha arvensis in different solvent 50% and 10% methanol, ethyl acetate, chloroform and was tested against human Cariogenic pathogens Streptococcus mutans...

  13. Anti-bacterial effect of Mentha spicata L. essential oil on eight standard species of gastrointestinal pathogens

    OpenAIRE

    Majid Zare Bidaki; Mina Arab; Mohtarame Khazaei; Ehsan Afkar

    2014-01-01

    Background and Aim: Nowadays resistance to antibiotics and their side effects has emerged as a worldwide problem. As a result, tend to use anti-bacterial compounds of plant origin has been increased. Mint plant scientifically called Mentha spicata L. is one of the plants which has many medicinal uses and its antibacterial effects is a matter of debate. We aimed to study antibacterial effects of Mentha spicata L essential oil on 8 standard bacterial species including Escherichia coli, Bacillus...

  14. A comparison Comparison between analgesic effects of aqueous ethanolic extract of mentha longifolia and morphine in male rats

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    Ezatollah Paknia

    2013-08-01

    Full Text Available Background and Aim: Long-term consumption of many drugs followed by reduction of their effectiveness has necessitated performing research on new analgesics .Thus, the present study was conducted to evaluate the analgesic effects of mentha longifolia and morphine in mice using writhing and hot plate tests. Materials and Methods: In this experimental study, 70 male rats were divided into 7 equal groups. The groups included the control, three experimental groups receiving 400, 800, or 1600 mg/kg of mentha extract and three experimental groups which received 2, 4, or 8 mg/kg of morphine .In order to measure pain, the two acceptable tests, writhing and hot plate tests, were applied. Pain scores were measured at 0, 15, 30, 45 or 60 min after administration of algogenic stimulus. Results: It was found that in hot plate test, only the dose of 1600mg/kg of Mentha extract after 60 minutes was significantly able to exert an analgesic effect (P<0.05. In wrighting test, mentha extract at different doses significantly reduced the number and time of wrightes in the rats, comparable to morphine (P<0.05. Conclusion: It seems that all doses of mentha extract in wrighting test have analgesic effects which indicate chronic pain inhibition of mentha hydroalcholic extract.

  15. α-Humulene and β-elemene from Syzygium zeylanicum (Myrtaceae) essential oil: highly effective and eco-friendly larvicides against Anopheles subpictus, Aedes albopictus, and Culex tritaeniorhynchus (Diptera: Culicidae).

    Science.gov (United States)

    Govindarajan, Marimuthu; Benelli, Giovanni

    2016-07-01

    Mosquitoes transmit serious pathogens and parasites to humans and animals, including malaria, dengue, Japanese encephalitis and filariasis. The extensive use of chemical pesticides leads to the development of resistance in mosquito vector populations and serious non-target effects on human health and the environment. Myrtaceae plants can be a useful reservoir of natural products effective against Culicidae young instars. In this research, we evaluated the mosquitocidal potential of the essential oil (EO) from Syzygium zeylanicum leaves against larvae of three mosquitoes of medical and veterinary importance, the malaria vector Anopheles subpictus, the dengue vector Aedes albopictus, and the Japanese encephalitis vector Culex tritaeniorhynchus. The chemical composition of the EO was analyzed by gas chromatography-mass spectroscopy. GC-MS revealed that the S. zeylanicum EO contained at least 18 compounds. The major chemical components were α-humulene (37.8.5 %) and β-elemene (10.7 %). The EO had a significant toxic effect against early third-stage larvae of An. subpictus, Ae. albopictus, and Cx. tritaeniorhynchus, with LC50 values of 83.11, 90.45, and 97.96 μg/ml, respectively. The two major constituents extracted from the S. zeylanicum EO were tested individually for acute toxicity against larvae of the three mosquito vectors. α-Humulene and β-elemene appeared highly effective against An. subpictus (LC50 = 6.19 and 10.26 μg/ml, respectively), followed by Ae. albopictus (LC50 = 6.86 and 11.15 μg/ml) and Cx. tritaeniorhynchus (LC50 = 7.39 and 12.05 μg/ml). Furthermore, the EO and its major components was safe towards the non-target fish Gambusia affinis; LC50 values were 20374.26, 1024.95, and 2073.18 μg/ml, respectively for EO, α-humulene and β-elemene. Overall, this study highlighted that the acute toxicity of S. zeylanicum EO towards mosquito larvae was mainly due to the presence of α-humulene and β-elemene. Furthermore, we pointed

  16. Ocorrência de Salmonella e coliformes de origem fecal na canela em pó (Cinnamomum cassia Blume a Cinnamomum zeylanicum Nees comercializada em Florianópolis, Santa Catarina, Brasil Salmonella and fecal coliforms in cinnamon (Cinnamomum cassia Blume and Cinnamomum zeylanicum Nees sold in the city of Florianópolis, Santa Catarina, Brazil

    Directory of Open Access Journals (Sweden)

    Jane Maria de S. Philippi

    1995-12-01

    Full Text Available Cem amostras de canela em pó de dez marcas diferentes comercializadas na cidade de Florianópolis, SC, foram submetidas à análise microbiológica, pesquisando-se Salmonella e coliformes de origem fecal. Em nenhuma amostra foi detectada Salmonella. Coliformes de origem fecal foram encontrados entre os valores The microbiological quality of a hundred samples of ten differents commercial brands of a ground cinnamon (Cinnamomum cassia Blume and Cinnamomum zeylanicum Nees from supermarkets in the city of Florianópolis, Brazil, was assessed. Salmonella and fecal coliforms were determined. Results were negative for Salmonella Fecal coliforms values ranged from 100 MPN per g. Fecal coliforms were detected in 37% of the cinnamon samples.

  17. Demonstration that limonene is the first cyclic intermediate in the biosynthesis of oxygenated p-menthane monoterpenes in Mentha piperita and other Mentha species

    Energy Technology Data Exchange (ETDEWEB)

    Croteau, R.; Kjonaas, R.

    1983-01-01

    The volatile oil of mature Mentha piperita (peppermint) leaves contains as major components the oxygenated p-menthane monoterpenes l-menthol (47%) and l-menthone (24%) as well as very low levels of the monoterpene olefins limonene (1%) and terpinolene (0.1%), which are considered to be probable precursors of the oxygenated derivatives. Immature leaves, which are actively synthesizing monoterpenes, produce an oil with comparatively higher levels of limonene approx.3%), and isolation of the pure olefin showed this compound to consist of approx.80% of the l-(4S)-enantiomer and approx.20% of the d-(4R)-enantiomer. The time course of incorporation of (U-/sup 14/C)sucrose into the monoterpenes of M. piperita shoot tips was consistent with the inital formation of limonene and its subsequent conversion to menthone via pulegone. d,l-(9-/sup 3/H)Limonene and (9,10-/sup 3/H)terpinolene were prepared and tested directly as precursors of oxygenated p-menthane monoterpenes in M. piperita shoot tips. Limonene was readily incorporated into pulegone, menthone, and other oxygenated derivatives, whereas terpinolene was not appreciably incorporated into these compounds. Similarly, d,l-(9-/sup 3/H)limonene was specifically incorporated into pulegone in Mentha pulegium and into the C-2-oxygenated derivative carvone in Mentha spicata, confirming the role of this olefin as the essential precursor of oxygenated p-menthane monoterpenes. Soluble enzyme preparations from the epidermis of immature M. piperita leaves converted the acyclic terpenoid precursor (1-/sup 3/H)geranyl pyrophosphate to limonene as the major cyclic product.

  18. Constituintes voláteis de Mentha pulegium L. e Plectranthus amboinicus (Lour.) Spreng Volatile constituents of Mentha pulegium L. and Plectranthus amboinicus (Lour.) Spreng

    OpenAIRE

    R.A. Oliveira; I.C.G Sá; Duarte, L P; F.F Oliveira

    2011-01-01

    Nesse trabalho foi investigado a composição química de óleos essenciais de duas plantas medicinais cultivadas em Ilhéus, Brasil, Plectranthus amboinicus (Lour.) Spreng. (hortelã-grosso) e Mentha pulegium L. (poejo). Os óleos essenciais foram extraídos das folhas frescas por hidrodestilação e foram analisados por CG/FID e CG-EM, na primavera e no inverno. P. amboinicus forneceu, tanto na primavera como no inverno, 0,10% de óleo sendo timol o componente majoritário. M. pulegium forneceu na prim...

  19. Mentha piperita as a pivotal neuro-protective agent against gamma irradiation induced DNA fragmentation and apoptosis: Mentha extract as a neuroprotective against gamma irradiation

    OpenAIRE

    Hassan, Hanaa A.; Hafez, Hani S.; Goda, Mona S.

    2012-01-01

    Ionizing radiation is classified as a potent carcinogen, and its injury to living cells, in particular to DNA, is due to oxidative stress enhancing apoptotic cell death. Our present study aimed to characterize and semi-quantify the radiation-induced apoptosis in CNS and the activity of Mentha extracts as neuron-protective agent. Our results through flow cytometry exhibited the significant disturbance and arrest in cell cycle in % of M1: SubG1 phase, M2: G0/1 phase of diploid cycle, M3: S phas...

  20. EVALUATION OF IN VITRO ANTIOXIDANT AND ANTIMI CROBIAL ACTIVITY OF MENTHA SPICATA L. LEAVES

    Directory of Open Access Journals (Sweden)

    Neelima R

    2014-11-01

    Full Text Available Different extracts of Mentha spicata L. leaves were investigated for their in vitroantioxidant and antimicrobial activities. In vitroantioxidant activity was evaluated for chloroform, ethanol and aqueous extracts for scavenging of DPPH, Superoxide and FRAP radicals. Antimicrobial activity of the extracts was evaluated against five selected bacterial strains by using cylinder plate assay.Concentration dependent antioxidant activity was observed for all the tested extracts. All the tested extracts of Mentha spicata showed considerable zone of inhibition against the tested bacterial strains. All the extracts showedsignificant zone of inhibition at a dose of 500μg/ml. Of the three extracts tested the highest zone of inhibition was shown by ethanolic extract against B.subtilisat the dose of 500μg/ml. All extracts exhibited antioxidant and antimicrobial activity in a dose dependent manner.

  1. Avaliação de substratos orgânicos na produção de mudas de hortelã (Mentha gracilis R. Br. e Mentha x villosa Huds. Evaluation of organic substrates on mint (Mentha gracilis R. Br. and Mentha x villosa Huds. seedling production

    Directory of Open Access Journals (Sweden)

    D Paulus

    2011-01-01

    Full Text Available A determinação de substratos alternativos viáveis para a produção de mudas de plantas medicinais é de grande relevância, pois o aproveitamento de resíduos de atividades agrícolas representa alternativa para problemas ambientais e sociais. Os experimentos foram realizados em ambiente protegido e a campo, na Universidade Tecnológica Federal do Paraná, Campus Dois Vizinhos, com o objetivo de avaliar substratos orgânicos para produção de mudas de hortelã para cultivo a campo. O delineamento experimental foi blocos casualizados, com três repetições, em esquema fatorial, sendo avaliados quatro substratos [esterco bovino + vermiculita + areia (1:1:1, esterco bovino + areia + solo (1:1:1, solo + vermiculita + cama-de-aviário (1:1:1 e substrato organo-mineral Plantmax® HA] e duas espécies de menta (Mentha gracilis e Mentha x villosa. Foram analisadas a altura da plantas e o número de folhas aos 07, 14 e 21 dias após o transplantio, índice de mortalidade, massa fresca e seca da parte aérea e das raízes. Não houve interação entre as espécies de menta e substratos para as variáveis analisadas. O substrato organo-mineral Plantmax® apresentou os melhores resultados para o número de folhas (8,44, altura (8,46 cm, produção de massa fresca (1,56 g planta-1, seca (0,23 g planta-1 e 100% de pegamento de mudas a campo o que para o produtor de menta é de grande interesse. Com base nos resultados obtidos, pode-se concluir que para a produção de mudas de Mentha gracilis e Mentha villosa o substrato Plantmax® HA é o mais recomendado.The determination of viable alternative substrates is of great importance for the production of medicinal seedlings since the use of residues from agricultural activities represents an alternative to solve environmental and social problems. The experiments were carried out in protected environment and in the field, located in the Federal Technological University of Paraná, Dois Vizinhos Campus, Paran

  2. Antimutagenic and anticancer activity of Al Madinah Alhasawy mint (Mentha longifolia) leaves extract.

    Science.gov (United States)

    Al-Ali, Khalil; Abdelrazik, Mohamad; Alghaithy, Abdulaziz; Diab, Atef; El-Beshbishy, Hesham; Baghdadi, Hussam

    2014-12-01

    Mentha is one of the genera of Lamiaceae family. The aim of the present study was to evaluate the antimutagenic and anticancer activity of the water and methanolic extract of Alhasawy mint (Mentha longifolia), that grown in Madina Province, western region, Saudi Arabia using three different bioassays namely; Brine shrimp bioassay, Ames mutagenicity bioassay using 3 Hist-Salmonella typhimurium strains of different mutations (TA98, TA97 and TA100) and 2 reference mutagenic drugs nitrosopiperidine (NP) and 2-amino-3-methylimidazo-quinolidine (IQ) and Mammalian cell lines bioassays using 2 different cell lines HepG2 and Vero cell lines. The plant extract showed an efficient antimutagenic activity against the studied bioassays in a directly proportional effect with concentration. PMID:26027170

  3. Antimutagenic and anticancer activity of Al Madinah Alhasawy mint (Mentha longifolia) leaves extract.

    Science.gov (United States)

    Al-Ali, Khalil; Abdelrazik, Mohamad; Alghaithy, Abdulaziz; Diab, Atef; El-Beshbishy, Hesham; Baghdadi, Hussam

    2014-12-01

    Mentha is one of the genera of Lamiaceae family. The aim of the present study was to evaluate the antimutagenic and anticancer activity of the water and methanolic extract of Alhasawy mint (Mentha longifolia), that grown in Madina Province, western region, Saudi Arabia using three different bioassays namely; Brine shrimp bioassay, Ames mutagenicity bioassay using 3 Hist-Salmonella typhimurium strains of different mutations (TA98, TA97 and TA100) and 2 reference mutagenic drugs nitrosopiperidine (NP) and 2-amino-3-methylimidazo-quinolidine (IQ) and Mammalian cell lines bioassays using 2 different cell lines HepG2 and Vero cell lines. The plant extract showed an efficient antimutagenic activity against the studied bioassays in a directly proportional effect with concentration.

  4. Bioactive properties of Mentha spicata L. infusions: a comparison between standard and reserve lots

    OpenAIRE

    Rita, Íngride; Pereira, Carla; Heleno, Sandrina A.; Ferreira, Isabel C.F.R.

    2015-01-01

    Mentha spicata L., commonly known as spearmint and belonging to the Lamiaceae family, is widely used as infusion due to its exquisite and outstanding flavour [1]. This medicinal and aromatic plant is also known for its antioxidant properties due to naturally occurring active compounds, such as phenolic compounds including flavonoids. [2] Recently, with the increased consumption of herbal infusions, several novel products are emerging and different lots with distinct composition...

  5. CHEMICAL AND BIOLOGICAL STUDY OF MENTHA SPICATAL ESSENTIAL OIL FROM IRAN

    Directory of Open Access Journals (Sweden)

    ABBAS HADJIAKHOONDI

    2000-07-01

    Full Text Available The essential oil from spearmint {Mentha spicata L., Labiatae was extracted by hydrodistillation, and analysed by GC and GC/MS. Twenty eight components were identified representing 90.14% of total oil composition. The major constituents were carvone (22.40%, linalool (11.25% and limonene (10.80%. The essential oil showed good activity against larvae of Anophel stephensi and Artemia salina.

  6. CHEMICAL AND BIOLOGICAL STUDY OF MENTHA SPICATAL ESSENTIAL OIL FROM IRAN

    OpenAIRE

    ABBAS HADJIAKHOONDI; NASRIN AGHEL; NASRIN ZAMANIZADEH-NADGAR; HASSAN VATANDOOST

    2000-01-01

    The essential oil from spearmint {Mentha spicata L., Labiatae) was extracted by hydrodistillation, and analysed by GC and GC/MS. Twenty eight components were identified representing 90.14% of total oil composition. The major constituents were carvone (22.40%), linalool (11.25%) and limonene (10.80%). The essential oil showed good activity against larvae of Anophel stephensi and Artemia salina.

  7. EVALUATION OF IN VITRO ANTIOXIDANT AND ANTIMI CROBIAL ACTIVITY OF MENTHA SPICATA L. LEAVES

    OpenAIRE

    Neelima R; Nagaraju N; Bhavani Y; Bandaru V Rao

    2014-01-01

    Different extracts of Mentha spicata L. leaves were investigated for their in vitroantioxidant and antimicrobial activities. In vitroantioxidant activity was evaluated for chloroform, ethanol and aqueous extracts for scavenging of DPPH, Superoxide and FRAP radicals. Antimicrobial activity of the extracts was evaluated against five selected bacterial strains by using cylinder plate assay.Concentration dependent antioxidant activity was observed for all the tested extracts. All the tested extra...

  8. Efektifitas Serbuk Daun Spearmint (Mentha spicata) Sebagai Repellent Terhadap Kecoa Rumah (Periplaneta americana)

    OpenAIRE

    Br Ginting, Mandasari

    2015-01-01

    American cockroach (Periplaneta americana) is mechanical vector which can bring agent of diseases. In Cockroach had ever been found about 40 microorganism so that can contaminate human food and cause some intestinal diseases even the allergic for human.Usually, People control the American cockroach by using chemical substances which persistent in environment if we use it continuously. The aim of this research was to analyze the effectivity of spearmint (Mentha spicata) leaves powde...

  9. Effect of Cadmium and Lead on Quantitative and Essential Oil Traits of Peppermint (Mentha piperita L.)

    OpenAIRE

    Shahram AMIRMORADI; Parviz Rezvani MOGHADDAM; Alireza KOOCHEKI; Shahnaz DANESH; Amir FOTOVAT

    2012-01-01

    Cadmium (Cd) and lead (Pb) are particularly noteworthy metals that can pollute the air, soil and water contributing to serious environmental problems. Tests were done on concentrations of Pb and Cd; treatments tested in the experiment were as follows; Cd concentrations (10, 20, 40, 60, 80, 100 ppm) and concentrations of Pb (100, 300, 600, 900, 1200, 1500 ppm) and control. Tests were done on Mentha piperita L. in a greenhouse, arranged as a randomized complete block design with three replicati...

  10. Chemical Composition of Essential Oilsof Thymus and Mentha Speciesand Their Antifungal Activities

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    Leo J. L. D. van Griensven

    2009-01-01

    Full Text Available The potential antifungal effects of Thymus vulgaris L., Thymus tosevii L., Mentha spicata L., and Mentha piperita L. (Labiatae essential oils and their components against 17 micromycetal food poisoning, plant, animal and human pathogens are presented. The essential oils were obtained by hydrodestillation of dried plant material. Their composition was determined by GC-MS. Identification of individual constituents was made by comparison with analytical standards, and by computer matching mass spectral data with those of the Wiley/NBS Library of Mass Spectra. MIC’s and MFC’s of the oils and their components were determined by dilution assays. Thymol (48.9% and p-cymene (19.0% were the main components of T. vulgaris, while carvacrol (12.8%, a-terpinyl acetate (12.3%, cis-myrtanol (11.2% and thymol (10.4% were dominant in T. tosevii. Both Thymus species showed very strong antifungal activities. In M. piperita oil menthol (37.4%, menthyl acetate (17.4% and menthone (12.7% were the main components, whereas those of M. spicata oil were carvone (69.5% and menthone (21.9%. Mentha sp. showed strong antifungal activities, however lower than Thymus sp. The commercial fungicide, bifonazole, used as a control, had much lower antifungal activity than the oils and components investigated. It is concluded that essential oils of Thymus and Mentha species possess great antifungal potential and could be used as natural preservatives and fungicides.

  11. Chemical Composition and Antibacterial Activity of the Essence oil of Peppermint (Mentha piperita L)

    OpenAIRE

    Z Izadi; M Esna-Ashari; G Ahmadvand; P. Davoodi; KH Piri

    2009-01-01

    Introduction & Objective: Peppermint (Mentha piperita L.) is a perennial herbaceous essence oil bearing plant which belongs to the Lamiaceae family. This plant is a valuable and important herb which has many therapeutic properties. Recent investigations have shown its excellent anti-irritable bowel syndrome effects. Other properties of this plant are anti-inflammatory, analgesic, promote menstrual flow, antipyretic, antiseptic and anti-rheumatoid effects. This investigation was conducted to s...

  12. Metabolic Profile of Offspring from Diabetic Wistar Rats Treated with Mentha piperita (Peppermint)

    OpenAIRE

    Barbalho, Sandra M.; Damasceno, Débora C; Ana Paula Machado Spada; Vanessa Sellis da Silva; Karla Aparecida Martuchi; Marie Oshiiwa; Flávia M. V. Farinazzi Machado; Claudemir Gregório Mendes

    2011-01-01

    This study aimed at evaluating glycemia and lipid profile of offspring from diabetic Wistar rats treated with Mentha piperita (peppermint) juice. Male offspring from nondiabetic dams (control group: 10 animals treated with water and 10 treated with peppermint juice) and from dams with streptozotocin-induced severe diabetes (diabetic group: 10 animals treated with water and 10 treated with peppermint juice) were used. They were treated during 30 days, and, after the treatment period, levels of...

  13. Sušenje lista paprene metvice (Mentha piperita) u elementarnom (tankom) sloju

    OpenAIRE

    Pliestić, Stjepan; Dobričević, Nadica

    2006-01-01

    List paprene metvice (Mentha piperita) bio je sušen u elemen-tarnom (tankom) sloju u sušnici na temperaturama zraka raspona od 35 do 60 °C. Porast temperature radnog medija (zraka) značajno je smanjio vrijeme sušenja lista. Podaci o sušenju lista paprene metvice analizirani su i u pogledu difuzije u periodima sušenja.

  14. Composition and antifungal activity of the essential oil of Mentha cervina from Portugal

    OpenAIRE

    Gonçalves, M. J.; Vicente, A. M.; C. Cavaleiro; Salgueiro, L.

    2007-01-01

    The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Mentha cervinacollected during the flowering and vegetative phases of the plants were investigated by GC and GC-MS. Quantitative differences were observed in the compositions, particularly in the amounts of pulegone (12.9–79.6%) and isomenthone (8.7–77.0%). Antifungal activity of the oils was evaluated by minimal inhibitory concentrations (MIC) and minimal lethal concentrations (MLC) against ...

  15. Effect of essential oils of Syzygium aromaticum and Cinnamomum zeylanicum and their major components on biofilm production in Staphylococcus aureus strains isolated from milk of cows with mastitis.

    Science.gov (United States)

    Budri, P E; Silva, N C C; Bonsaglia, E C R; Fernandes Júnior, A; Araújo Júnior, J P; Doyama, J T; Gonçalves, J L; Santos, M V; Fitzgerald-Hughes, D; Rall, V L M

    2015-09-01

    Bovine mastitis is an inflammation of the mammary glands of cows and causes significant economic losses in dairy cattle. Staphylococcus aureus is one of the microorganisms most commonly isolated. Novel agents are required in agricultural industries to prevent the development of mastitis. The production of biofilm by Staph. aureus facilitates the adhesion of bacteria to solid surfaces and contributes to the transmission and maintenance of these bacteria. The effect of the essential oils of Syzygium aromaticum (clove; EOSA) and Cinnamomum zeylanicum (cinnamon; EOCZ) and their major components, eugenol and cinnamaldehyde, on Staph. aureus biofilm formation on different surfaces was investigated. The results showed a significant inhibition of biofilm production by EOSA on polystyrene and stainless steel surfaces (69.4 and 63.6%, respectively). However, its major component, eugenol, was less effective on polystyrene and stainless steel (52.8 and 19.6%, respectively). Both EOCZ and its major component, cinnamaldehyde, significantly reduced biofilm formation on polystyrene (74.7 and 69.6%, respectively) and on stainless steel surfaces (45.3 and 44.9%, respectively). These findings suggest that EOSA, EOCZ, and cinnamaldehyde may be considered for applications such as sanitization in the food industry.

  16. Effect of essential oils of Syzygium aromaticum and Cinnamomum zeylanicum and their major components on biofilm production in Staphylococcus aureus strains isolated from milk of cows with mastitis.

    Science.gov (United States)

    Budri, P E; Silva, N C C; Bonsaglia, E C R; Fernandes Júnior, A; Araújo Júnior, J P; Doyama, J T; Gonçalves, J L; Santos, M V; Fitzgerald-Hughes, D; Rall, V L M

    2015-09-01

    Bovine mastitis is an inflammation of the mammary glands of cows and causes significant economic losses in dairy cattle. Staphylococcus aureus is one of the microorganisms most commonly isolated. Novel agents are required in agricultural industries to prevent the development of mastitis. The production of biofilm by Staph. aureus facilitates the adhesion of bacteria to solid surfaces and contributes to the transmission and maintenance of these bacteria. The effect of the essential oils of Syzygium aromaticum (clove; EOSA) and Cinnamomum zeylanicum (cinnamon; EOCZ) and their major components, eugenol and cinnamaldehyde, on Staph. aureus biofilm formation on different surfaces was investigated. The results showed a significant inhibition of biofilm production by EOSA on polystyrene and stainless steel surfaces (69.4 and 63.6%, respectively). However, its major component, eugenol, was less effective on polystyrene and stainless steel (52.8 and 19.6%, respectively). Both EOCZ and its major component, cinnamaldehyde, significantly reduced biofilm formation on polystyrene (74.7 and 69.6%, respectively) and on stainless steel surfaces (45.3 and 44.9%, respectively). These findings suggest that EOSA, EOCZ, and cinnamaldehyde may be considered for applications such as sanitization in the food industry. PMID:26142866

  17. Influence of Different Temperatures and Times on Antiradical Properties of Zataria multiflora Boiss. and Cinnamon zeylanicum Essential Oils by Using DPPH Method

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    Habibeh KORDSARDOUEI

    2013-12-01

    Full Text Available The oxidation of fats and oils has a key role in the reduction of the nutritional and organoleptic properties of foodstuffs. Nowadays, there is a tendency to create to use natural preservatives, such as essential oils, for antioxidant, antiradical and antimicrobial properties in foodstuffs. In this study, the effect of thermal processing on the antiradical activities of Zataria multiflora Boiss. (ZMEO and Cinnamon zeylanicum (CZEO essential oils is checked. Antiradical activities were measured with a 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH– assay at 3 temperatures (100, 140 and 180 °C and at 3 different time intervals (1, 2 and 3 h. The EC50 of ZMEO and CZEO were 4026.67 ± 2.2 and 2605.01 ± 15.57 ppm, respectively, at 25 °C. The 2 essences showed various reactions and characteristics at different temperatures (100, 140 and 180 °C and time ranges (1, 2 and 3 h. Maximum and minimum antiradical properties were observed for ZMEO at respectively, 140 and 180 °C after 1 h heating.

  18. Assessment of mint (Mentha spp. species for large-scale production of plantlets by micropropagation

    Directory of Open Access Journals (Sweden)

    Hugo Teixeira Gomes

    2015-10-01

    Full Text Available Species of the genus Mentha produce essential oils which are widely used in pharmaceutical and cosmetic industries. Current study evaluates the potential for in vitro propagation and estimates mass production of plantlets of Mentha species. Nine species (M. piperita, M. suaveolens, M. canadensis, M. longiflora, M. aquatica, M. arvensis, Mentha x gracilis, M. gracilis and M. spicata were propagated with five successive 30-day subcultures in MS medium supplemented with NAA (0.05 µM and BAP (4.4 µM. Shoots were rooted in MS with IBA, IAA or NAA (0.0; 0.25; 0.5; 2.5 or 5.0 µM. The rooted plantlets were finally acclimatized in a greenhouse. Studied species increased in multiplication rates between 4.2 and 9.0-fold per month. M. piperita, M. longiflora, M. arvensis, M. x gracilis and M. gracilis showed the greatest potential for plantlet production since the estimated production varied between 6,000 and 27,000 plantlets after five 30-days subcultures. The addition of auxin to the medium did not influence root induction. However, IAA at a concentration of 5 µM provided the best results for root length and fresh weight, with averages 11.1 cm and 0.16 g, respectively. Survival of plantlets reached 100% during the greenhouse acclimatization process.

  19. Analysis of genetic variability and relationships among Mentha L. using the limonene synthase gene, LS.

    Science.gov (United States)

    Wang, Hai Tang; Yu, Xu; Liu, Yan; Liang, Cheng-Yuan; Li, Wei-Lin

    2013-07-25

    The genus Mentha comprises a group of aromatic plants with worldwide distribution. Because of frequent interspecific hybridization, the genetic relationships within the genus are not clearly understood. Limonene synthase, which catalyses the first committed step in the essential oil monoterpene biosynthetic pathway, is considered to be a possible rate limiting enzyme. With the homology-based cloning method, primers were designed according to cDNA sequence to amplify full-length DNA sequences in 13 Mentha samples from five species, using Perilla as an outgroup. Analyses of gene structure, length variation, GC-content, Ts/Tv ratio and evolutionary diversity were carried out. Consensus phylogenetic trees were obtained using maximum likelihood, neighbor-joining, and maximum parsimony, respectively, based on the full-length genomic DNA sequences, complete ORF coding sequences and predicted amino acid sequences. The results presented here based on the sequence of MhLS provide the first credibly supported genetic relationships for Mentha, which enables a basis for further mint taxonomy, cultivation and breeding.

  20. Antiproliferative activity of flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the MCF7, KB, and NIH/3T3 cell lines.

    Science.gov (United States)

    Nedel, Fernanda; Begnini, Karine; Carvalho, Pedro Henrique de Azambuja; Lund, Rafael Guerra; Beira, Fátima T A; Del Pino, Francisco Augusto B

    2012-11-01

    This study assessed the antiproliferative effect in vitro of the flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the human breast adenocarcinoma (MCF-7), human mouth epidermal carcinoma (KB), and mouse embryonic fibroblast (NIH 3T3) cell lines, using sulforhodamine B (SRB) assay. A cell density of 2×10(4)/well was seeded in 96-well plates, and samples at different concentrations ranging from 10 to 500 mg/mL were tested. The optical density was determined in an ELISA multiplate reader (Thermo Plate TP-Reader). Results demonstrated that the hexane extract presented antiproliferative activity against both the tumor cell lines KB and MCF-7, presenting a GI(50) (MCF-7=13.09 mg/mL), TGI (KB=37.76 mg/mL), and IL(50) (KB=291.07 mg/mL). Also, the hexane extract presented antiproliferative activity toward NIH 3T3 cells GI(50) (183.65 mg/mL), TGI (280.54 mg/mL), and IL(50) (384.59 mg/mL). The results indicate that the flower hexane extract obtained from M. spicata associated with M. rotundifolia presents an antineoplastic activity against KB and MCF-7, although an antiproliferative effect at a high concentration of the extract was observed toward NIH 3T3. PMID:23066647

  1. Antiproliferative activity of flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the MCF7, KB, and NIH/3T3 cell lines.

    Science.gov (United States)

    Nedel, Fernanda; Begnini, Karine; Carvalho, Pedro Henrique de Azambuja; Lund, Rafael Guerra; Beira, Fátima T A; Del Pino, Francisco Augusto B

    2012-11-01

    This study assessed the antiproliferative effect in vitro of the flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the human breast adenocarcinoma (MCF-7), human mouth epidermal carcinoma (KB), and mouse embryonic fibroblast (NIH 3T3) cell lines, using sulforhodamine B (SRB) assay. A cell density of 2×10(4)/well was seeded in 96-well plates, and samples at different concentrations ranging from 10 to 500 mg/mL were tested. The optical density was determined in an ELISA multiplate reader (Thermo Plate TP-Reader). Results demonstrated that the hexane extract presented antiproliferative activity against both the tumor cell lines KB and MCF-7, presenting a GI(50) (MCF-7=13.09 mg/mL), TGI (KB=37.76 mg/mL), and IL(50) (KB=291.07 mg/mL). Also, the hexane extract presented antiproliferative activity toward NIH 3T3 cells GI(50) (183.65 mg/mL), TGI (280.54 mg/mL), and IL(50) (384.59 mg/mL). The results indicate that the flower hexane extract obtained from M. spicata associated with M. rotundifolia presents an antineoplastic activity against KB and MCF-7, although an antiproliferative effect at a high concentration of the extract was observed toward NIH 3T3.

  2. Coatings comprising chitosan and Mentha piperita L. or Mentha × villosa Huds essential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit.

    Science.gov (United States)

    Guerra, Ingrid Conceição Dantas; de Oliveira, Priscila Dinah Lima; Pontes, Alline Lima de Souza; Lúcio, Ana Sílvia Suassuna Carneiro; Tavares, Josean Fechine; Barbosa-Filho, José Maria; Madruga, Marta Suely; de Souza, Evandro Leite

    2015-12-01

    In this study, we evaluated the efficacy of coatings comprising shrimp chitosan (CHI) and Mentha piperita L. (MPEO) or Mentha × villosa Huds (MVEO) essential oils to control mold infections caused by Aspergillus niger, Botrytis cinerea, Penicillium expansum and Rhizopus stolonifer in cherry tomato fruits (Solanum lycopersicum L.) during storage at room temperature (25°C for 12 days) and low temperature (12°C for 24 days). The effects of the coatings on the physicochemical and sensory characteristics of cherry tomato fruits during storage were also assessed. The minimum inhibitory concentration (MIC) of CHI against all test fungi was 8 mg/mL, whereas the MIC for both MPEO and MVEO was 5 μL/mL. Combinations of CHI at 4 mg/mL and MPEO or MVEO at 2.5 or 1.25 μL/mL strongly inhibited the mycelial growth and spore germination of target fungi. The coatings comprising CHI and MPEO or CHI and MVEO at the different tested concentrations delayed the growth of decay-causing fungi in artificially contaminated tomato fruit during storage at either room temperature or low temperature. The assayed coatings preserved the quality of cherry tomato fruit during storage, in terms of physicochemical and sensory attributes. These results indicate that coatings comprising CHI and MPEO or CHI and MVEO represent promising postharvest treatments to prevent common postharvest mold infections in cherry tomato fruit during storage without affecting the quality of the fruit.

  3. Coatings comprising chitosan and Mentha piperita L. or Mentha × villosa Huds essential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit.

    Science.gov (United States)

    Guerra, Ingrid Conceição Dantas; de Oliveira, Priscila Dinah Lima; Pontes, Alline Lima de Souza; Lúcio, Ana Sílvia Suassuna Carneiro; Tavares, Josean Fechine; Barbosa-Filho, José Maria; Madruga, Marta Suely; de Souza, Evandro Leite

    2015-12-01

    In this study, we evaluated the efficacy of coatings comprising shrimp chitosan (CHI) and Mentha piperita L. (MPEO) or Mentha × villosa Huds (MVEO) essential oils to control mold infections caused by Aspergillus niger, Botrytis cinerea, Penicillium expansum and Rhizopus stolonifer in cherry tomato fruits (Solanum lycopersicum L.) during storage at room temperature (25°C for 12 days) and low temperature (12°C for 24 days). The effects of the coatings on the physicochemical and sensory characteristics of cherry tomato fruits during storage were also assessed. The minimum inhibitory concentration (MIC) of CHI against all test fungi was 8 mg/mL, whereas the MIC for both MPEO and MVEO was 5 μL/mL. Combinations of CHI at 4 mg/mL and MPEO or MVEO at 2.5 or 1.25 μL/mL strongly inhibited the mycelial growth and spore germination of target fungi. The coatings comprising CHI and MPEO or CHI and MVEO at the different tested concentrations delayed the growth of decay-causing fungi in artificially contaminated tomato fruit during storage at either room temperature or low temperature. The assayed coatings preserved the quality of cherry tomato fruit during storage, in terms of physicochemical and sensory attributes. These results indicate that coatings comprising CHI and MPEO or CHI and MVEO represent promising postharvest treatments to prevent common postharvest mold infections in cherry tomato fruit during storage without affecting the quality of the fruit. PMID:26313246

  4. In-Vivo Antioxidant activity of ethanolic extract of Mentha pulegium leaf against CCl4 induced toxicity in rats

    Institute of Scientific and Technical Information of China (English)

    Sachin Jain; Dinesh Kumar Jain; Neelam Balekar

    2012-01-01

    Objective: To evaluate the in-vivo antioxidant potential of ethanolic extract of Mentha Pulegium against CCl4 induced toxicity in rats. Methods: Animals were treated with plant extract for 7 days and then toxicity was induced with a single CCl4 intraperitoneal injection. Pre-treatment with 600 mg/kg (p.o.) of ethanolic extract of Mentha Pulegium improved the glutathione, SOD, catalase, and peroxidase levels significantly as compared to control group. Results: The present studies revealed that Mentha Pulegium has significant in-vivo antioxidant activity and can be used to protect tissue from oxidative stress. The result showed that the activities of glutathione, SOD, catalase and peroxidase in group treated with CCl4 declined significantly than that of normal group. Conclusion: Ethanolic extract of Mentha Pulegium in the dose of 600 mg/kg, p.o., has improved the glutathione, SOD, catalase, and peroxidase levels significantly, which were comparable with Liv 52. Based on this study we conclude that Ethanolic extract of MenthaPulegium possesses in vivo antioxidant activity and can be employed in protecting tissue from oxidative stress.

  5. Variabilité chimique et intérêt économique des huiles essentielles de deux menthes sauvages : Mentha pulegium (Fliou) et Mentha rotundifolia (Domrane) de l’ouest algérien.

    OpenAIRE

    TAALBI, Amina

    2016-01-01

    Notre travail s’est basé principalement sur une étude de la variabilité chimique et l’intérêt économique des huiles essentielles de deux menthes sauvages de l’ouest algérien: Mentha pulegium et Mentha rotundifolia. Ces deux menthes, largement répandue en Algérie, de la famille des Lamiaceae sont connus dans le monde pour leurs propriétés thérapeutiques (antiseptique, antinévralgique, analgésique…) et l’intérêt économique de leurs huiles essentielles....

  6. Influência do manejo de poda no crescimento, produção de biomassa e teor de óleo essencial de Mentha arvensis e Mentha suaviolens em sistema agroflorestal

    OpenAIRE

    Antunes, Jonai; Souza, R. Edenilson; Paulus, Dalva; Devens, Gilmarise

    2015-01-01

    Mentha arvensis e Mentha suaveolens são plantas medicinais muito apreciadas por suas características farmacêuticas, cosméticas e alimentícias. O objetivo deste trabalho foi avaliar a melhor época de poda, produção e teor de óleo essencial de menta em sistema agroflorestal. O trabalho foi desenvolvido na propriedade de um agricultor no município de Dois Vizinhos no estado do Paraná, Brasil, no período de fevereiro a novembro de 2014. O delineamento experimental utilizado foi o de blocos ao aca...

  7. HS-SPME-GC×GC-qMS volatile metabolite profiling of Chrysolina herbacea frass and Mentha spp. leaves.

    Science.gov (United States)

    Cordero, Chiara; Zebelo, Simon Atsbaha; Gnavi, Giorgio; Griglione, Alessandra; Bicchi, Carlo; Maffei, Massimo E; Rubiolo, Patrizia

    2012-02-01

    Headspace solid-phase microextraction (HS-SPME) comprehensive two-dimensional (2D) gas chromatography combined with quadrupole-mass spectrometry (GC×GC-qMS) with dedicated comparative data elaboration was applied to separate chemical patterns arising from the interaction between some Mentha species and the herbivore Chrysolina herbacea, also known as the mint bug. Upon feeding on different Mentha species (Mentha spicata L., Mentha × piperita L. and Mentha longifolia L.), C. herbacea produced frass (faeces) which were characterized by a typical volatile fraction. HS-SPME GC×GC-qMS analysis of the complex volatile fraction of both mint leaf and C. herbacea frass was submitted to advanced fingerprinting analysis of 2D chromatographic data. 1,8-Cineole, found in the leaves of all the Mentha species examined, was oxidized, and C. herbacea frass yielded high rates of several hydroxy-1,8-cineoles, including 2α-hydroxy-, 3α-hydroxy-, 3β-hydroxy- and 9-hydroxy-1,8-cineole. Upon insect feeding, several unknown oxidized monoterpenes, a p-menthane diol and three unknown phenylpropanoids were also detected in the frass volatiles. In M. longifolia, the occurrence of the monoterpene piperitenone oxide was found to be toxic and associated with insect death. The results of this work show that high throughput techniques such as HS-SPME and GC×GC-qMS fingerprint analysis are ideal tools to analyze complex volatile matrices, and provide a sensitive method for the direct comparison and chemical visualization of plant and insect emitted volatile components. PMID:22143743

  8. HS-SPME-GC×GC-qMS volatile metabolite profiling of Chrysolina herbacea frass and Mentha spp. leaves.

    Science.gov (United States)

    Cordero, Chiara; Zebelo, Simon Atsbaha; Gnavi, Giorgio; Griglione, Alessandra; Bicchi, Carlo; Maffei, Massimo E; Rubiolo, Patrizia

    2012-02-01

    Headspace solid-phase microextraction (HS-SPME) comprehensive two-dimensional (2D) gas chromatography combined with quadrupole-mass spectrometry (GC×GC-qMS) with dedicated comparative data elaboration was applied to separate chemical patterns arising from the interaction between some Mentha species and the herbivore Chrysolina herbacea, also known as the mint bug. Upon feeding on different Mentha species (Mentha spicata L., Mentha × piperita L. and Mentha longifolia L.), C. herbacea produced frass (faeces) which were characterized by a typical volatile fraction. HS-SPME GC×GC-qMS analysis of the complex volatile fraction of both mint leaf and C. herbacea frass was submitted to advanced fingerprinting analysis of 2D chromatographic data. 1,8-Cineole, found in the leaves of all the Mentha species examined, was oxidized, and C. herbacea frass yielded high rates of several hydroxy-1,8-cineoles, including 2α-hydroxy-, 3α-hydroxy-, 3β-hydroxy- and 9-hydroxy-1,8-cineole. Upon insect feeding, several unknown oxidized monoterpenes, a p-menthane diol and three unknown phenylpropanoids were also detected in the frass volatiles. In M. longifolia, the occurrence of the monoterpene piperitenone oxide was found to be toxic and associated with insect death. The results of this work show that high throughput techniques such as HS-SPME and GC×GC-qMS fingerprint analysis are ideal tools to analyze complex volatile matrices, and provide a sensitive method for the direct comparison and chemical visualization of plant and insect emitted volatile components.

  9. Mentha piperita micropropagation and volatil constituents evaluation Micropropagación de plantas de Mentha piperita y evaluación de sus constituyentes volátiles

    Directory of Open Access Journals (Sweden)

    Fernández Gloria Azucena

    1999-06-01

    Full Text Available The use of in vitro plant tissue culture techniques for the propagation of aromatic and medicinal species, could play very promising role for the economy of small farmers, due to the high potential of industrialization of their essential oils. In this work is shown how the tissue culture technique has been used for the propagation of Mentha piperita, as its essential oil is widely used in the flavor and fragrance industry. Mints from different sources were studied, and evaluation of the yield and quality of the essential oil was carry out, before and after the micropropagation by plant tissue culture. Several basal mediums with different proportion of plant growth regulators and vitamins were tested in order to maximize the propagation results. The constituents of the essential oil were quantified by Gas Chromatography. Lin & Staba (1962 basal medium, supplemented with BAP, and some vitamins, was the best for mints species micropropagation. The yield and quality of essential oil produced by plant tissue culture and its mother plant were very similar. La aplicación de técnicas de cultivo de tejidos vegetales in vitro en especies aromáticas y medicinales puede constituirse en un renglón importante para la economía de pequeños agricultores, debido a su alto potencial de comercialización y producción. Se pretende en este trabajo demostrar una vía de aplicación semiindustrial para cultivar masivamente especies vegetales de Mentha piperita, debido a la aceptación y consumo mundial de su aceite esencial. Se estudiaron diferentes variedades de Mentha para evaluar su rendimiento de producción de aceite esencial y su calidad, antes y después de la micropropagación. Para lograr el objetivo propuesto, se ensayaron diversos me-dios de cultivo y sus respectivos balances de fitohormonas y vitaminas para lograr el máximo de multiplicación. Además se caracterizó el aceite esencial por medio de la técnica de análisis instrumental de

  10. Comparative study of kinetics of adsorption of methylene blue from aqueous solutions using cinnamon plant (Cinnamonum zeylanicum leaf powder and pineapple (Ananas comosus peel powder

    Directory of Open Access Journals (Sweden)

    Satish Dnyandeo Patil

    2012-07-01

    Full Text Available Batch adsorption of methylene blue (MB onto Cinnamon plant (Cinnamonum Zeylanicum leaf powder (CPLP and Pineapple (Ananas Comosus peel powder (PPP was investigated. Different parameters such as initial sorbate concentration, adsorbent dosage, pH, contact time, agitation speed, temperature and particle size. All isotherm models were found to be best fitting with high values of regression coefficient i.e. for Langmuir (R2 = 0.989 to 0.994 for CPLP and 0.993 to 0.995 for PPP, for Freundlich (R2 = 0.996 to 0.998 for CPLP and 0.995 for PPP and for Temkin (R2 = 0.983 to 0.995 for CPLP and 0.984 to 0.989 for PPP. The monolayer (maximum adsorption capacities (qm were found to be 250 and 333.333 mg/g for CPLP and PPP respectively. Lagergen pseudo -second order model best fits the kinetics of adsorption (R2 = 0.999 for CPLP and 1 for PPP. The amount of dye adsorbed at equilibrium qe(the obtained from Lagergen pseudo -second order model were found to be nearly same with the experimental data. Intra particle diffusion plot showed boundary layer effect and larger intercepts indicates greater contribution of surface sorption in rate determining step. Adsorption was found to be directly proportional to pH and temperature but inversely proportional to particle size. Thermodynamic analysis (∆G, ∆H and ∆S values showed adsorption was favourable, spontaneous, endothermic physisorption and increased disorder and randomness at the solid- solution interface of MB with the adsorbents. The forward rate constant was much higher than reverse rate constant suggesting dominance of rate of adsorption. PPP was found to be better adsorbent than CPLP.

  11. Modulatory Effects of Mentha spicata (Linn.) against 4 Nitroquinoline-1- Oxide Induced Chromosome Damage and Oxidative Stress in Mice

    OpenAIRE

    Ponnan Arumugam; Siva Kamalakannan; Marudhamuthu Murugan

    2015-01-01

    Mentha spicata is a common medicinal and edible plant in India and used in the ayurvedic system of medicine to treat various ailments including as a memory enhancer. The present study is aimed to evaluate the modulatory effects of Mentha spicata (Linn.) against 4?nitroquinoline- 1-oxide (4-NQO) induced chromosome damage and oxidative stress in mice. Experiments were conducted with eight groups of either sex of mice. Ethanol extract (EE) of three doses (80, 160 and 320 mg/kg body weight-bw) wi...

  12. 349 estudios morfo-anatómicos de gineceo y fruto en especies de mentha (lamiaceae) de argentina

    OpenAIRE

    Bonzani, Norma; Bravi, Viviana; Barbosa, Gloria

    2012-01-01

    Se realizaron estudios morfológicos y anatómicos de gineceo y fruto, utilizando microscopía óptica y electrónica de barrido, en especies de Mentha (sect. Mentha L.y sect. Pulegium (Mill.) Coss. & Germ.). Se registran por primera vez para el género algunas novedades sobre el gineceo, histología del nectario, microesculturas en el epicarpo, presencia de ceras epicuticulares en el hilo y la morfología de los cristaloides. Se establecieron cuatro tipos de microesculturas en núculas maduras. L...

  13. Effect of Mentha Piperita L. Powder on the Overall Acceptability, Coliforms, and Molds and Yeasts Counts of Borujerd Domestic Cheese

    OpenAIRE

    FADAEİ NOGHANİ, Vajiheh; SAMETİ, Sadrieh

    2015-01-01

    Abstract. In this study, the antimicrobial effects of plant powder Mentha Piperita L. in at the levels of 0.1, 0.2 and 0.3 % on preventing the growth of mold, yeast and coliforms, as well as the, overall acceptability, pH and acidity of Borujerd domestic cheese samples during the cold storage were studied. The findings showed that with increasing the percentage of Mentha Piperita L. powder in cheese samples, the number of coliforms and mold and yeast, titratable acidity and overall acceptabil...

  14. Identifizierung von etherischen Ölen mit antioxidativer Kapazität in der Gattung Mentha L.

    OpenAIRE

    Sitzmann, Judith

    2010-01-01

    14 anhand chemotaxonomischer Merkmale bestimmte Mentha-Arten bzw. Hybriden wiesen hinsichtlich ihrer etherischen Ölgehalte und -zusammensetzungen deutliche Unterschiede auf. In zwei in vitro-Testsystemen zur Prüfung der antioxidativen Kapazität (Lipidperoxidation im Blutplasma und ABTS-Entfärbeassay) erzielten die Öle von Minzen mit höheren D-Germacren-, Piperitonoxid- und Piperitenonoxid-Gehalten gute Ergebnisse. Alle untersuchten Mentha-Spezies zeigten eine grundsätzliche Eignung für den ko...

  15. Estudos de microscopia óptica e de microscopia eletrônica de varredura em folhas de Mentha spicata e de Mentha spicata x suaveolens (Lamiaceae Optical microscopy studies and scanning electron microscopy in leaf the Mentha spicata and Mentha spicata x suaveolens (Lamiaceae

    Directory of Open Access Journals (Sweden)

    Maria Bernadete Gonçalves Martins

    2002-12-01

    Full Text Available O presente trabalho tem como objetivo realizar um estudo de anatomia foliar por meio de microscopia óptica e de microscopia eletrônica de varredura em Mentha spicata L. e Mentha spicata X suaveolens, caracterizando histologicamente a lâmina foliar. Secções transversais e paradérmicas da região mediana do limbo foliar mostraram a presença de epiderme unisseriada, coberta por uma fina camada de cutícula, apresentando tricomas glandulares do tipo capitado e peltado e não glandulares unisseriados multicelulares, não ramificados. O mesofilo de ambas as espécies é dorsiventral, com parênquima paliçádico uniestratificado, com células alongadas e rico em inclusões citoplasmáticas. O parênquima lacunoso é formado por três a quatro camadas de células irregulares. Os tricomas capitados presentes são classificados como do tipo I, e apresentam-se com uma célula basal, uma célula peduncular e uma grande célula apical, cujo formato varia de circular a piriforme. Os tricomas peltados consistem de uma célula basal, uma célula peduncular curta, larga e unicelular, com paredes externas cutinizadas e uma cabeça grande multicelular com 12 células secretoras, distribuídas radialmente em dois círculos concêntricos, o central com 4 células e o externo com 8 células, as quais acumulam o produto da secreção em uma cavidade entre a cutícula e as células secretoras; o pé do tricoma glandular está inserido em 11 células epidérmicas. Há predominância de tricomas capitados em relação aos tricomas peltados em ambas as espécies de Mentha.The objective of the present work is to make a study of leaf anatomy through optic microscopy and eletronic microscopy of scanning in Mentha spicata L. and Mentha spicata X suaveolens, characterizing the leaf blade histology. Cross and paradermic sections of the leaf, showed the presence of uniseriate epidermal cells covered by a fine cuticle layer, presenting gland trichomes of multicellular

  16. Artificial Autotetraploidy Induction Possibility of Two Iranian Endemic Mint (Mentha mozaffarianii Ecotypes

    Directory of Open Access Journals (Sweden)

    Askar GHANI

    2014-06-01

    Full Text Available The present study was conducted to polyploidy possibility induction of two Iranian endemic mint (Mentha mozaffarianii ecotypes. For this purpose, three experiments were done. The first experiment was factorial, based on completely randomized design with three factors and three replications that rhizomes were used for treatment. The first factor including different colchicine concentrations (0, 0.025, 0.05, 0.1 and 0.2% that 0 as control. The second factor including two Mentha ecotypes (Ecotype A: Kamarej region and Ecotype B: Pirmohlat region and the third factors consist of two soaking time (6 h and 12 h. In second experiment, apical meristem was treated. The factorial experiment based on randomized completely design with two factors and five replications. The factors including different colchicine concentrations (0, 0.035, 0.07, 0.15, 0.3 and 0.7% and two ecotypes. In the third experiment, colchicine as combined with irrigation was applied. For this purpose, a factorial experiment in randomized complete design with two factors (colchicine concentrations: 0, 0.025, 0.012, 0.006% and two mint ecotypes and three replications was conducted. At the end of all experiments, survival rate and tetraploidy percentage (by morphological change, stomata traits, flow cytometry and chromosome counts were measured. The results showed that different treatment had significant effects on survival percent on all experiments and increasing of colchicine concentration caused decreasing plants survival. On the other hand, tetraploidy changes only in the first experiments were observed. Only in 0.025% colchicine treatment with 6 h soaking time on ecotype A, 12.66% tetraploidy was identified. Totally, it is seems that Mentha mozaffarianiihardly response to colchicine for tetraploidy induction.

  17. Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes.

    Science.gov (United States)

    Maggiore, Marina A; Albanese, Adriana A; Gende, Liesel B; Eguaras, Martín J; Denegri, Guillermo M; Elissondo, María Celina

    2012-03-01

    The aim of the present work was to determine the in vitro effect of Mentha piperita and Mentha pulegium essential oils against Echinococcus granulosus and to compare the effectiveness of both oils according to the exposure time and concentration. Although both treatments had a protoscolicidal effect, M. pulegium had a considerably stronger effect than M. piperita. Essential oil of M. pulegium produced dose- and time-dependent effects. Maximal protoscolicidal effect was observed after 12 days of incubation and reached 0% after 18 days. This lack of viability was proved during the determination of infectivity into mice. Essential oil of M. piperita produced only a time-dependent effect. At 24 days p.i., the viability of protoscoleces decreased to approximately 50%. Scanning and transmission electron microscopy (SEM and TEM) demonstrated the drug-induced ultrastructural damage. On the other hand, a loss of turgidity was detected in all M. pulegium-treated cysts respective of the drug concentration. There was a correlation between the intensity of damage and the concentration of the essential oil assayed. Studies by SEM revealed that the germinal layer of treated cysts lost the feature multicellular structure. M. pulegium essential oil showed piperitone oxide as main compound in their composition, and we suggest that this component could be responsible of the markedly anthelmintic effect detected. Our data suggest that essential oils of Mentha spp. can be a promising source of potential protoscolicidal agents. The isolation of active anthelmintic constituents is in progress and may lead to the discovery of compounds with improved therapeutic value.

  18. Influência de citocininas na micropropagação de Mentha x gracilis Sole Cytokinin influence on Mentha x gracilis Sole micropropagation

    Directory of Open Access Journals (Sweden)

    T.M.B Garlet

    2011-01-01

    Full Text Available Mentha x gracilis Sole é um híbrido que produz óleos essenciais ricos em monoterpenos. Tendo em vista a propagação clonal desta planta, segmentos nodais provenientes de plantas assépticas, foram cultivados em meio de Murashige e Skoog (MS suplementado com 0; 0,5; 1,0 e 2,0 µM de cinetina, benzilaminopurina (BAP ou thidiazuron (TDZ. Após 30 dias, as plantas foram transferidas para meio MS não suplementado com citocinina. Os melhores resultados foram obtidos em meio suplementado com 2 µM de TDZ, mostrando ser método viável para a produção rápida de grande número de mudas. Após a transferência das plantas para a casa de vegetação, as plantas propagadas com TDZ apresentam maior número de tricomas glandulares.Mentha x gracilis Sole is a hybrid that produces essential oils rich in monoterpenes. Aimed at the clonal propagation of this plant, nodal segments from aseptic plants were cultured in Murashige and Skoog (MSO medium supplemented with 0; 0.5; 1.0 and 2.0 µM kinetin, benzyl adenine (BAP or thidiazuron (TDZ. After 30 days, plants were transferred to MOS medium without cytokinin supplementation. The best results were obtained in medium supplemented with 2 µM TDZ, which proved to be a viable method for the rapid production of a large number of seedlings. After transference to the greenhouse, plants propagated with TDZ had a larger number of glandular trichomes.

  19. Essential oil composition of sixteen elite cultivars of Mentha from western Himalayan region, India

    OpenAIRE

    RAJENDRA C. PADALIA

    2013-01-01

    The hydrodistilled essential oils of 16 cultivars of Mentha, viz. M. arvensis L., M. spicata L. and M. citrata Ehrh., were analysed and compared by gas chromatography and gas chromatography-mass spectrometry. Fifty-seven constituents representing 92.8-99.8% of the total essential oil composition were identified. Monoterpenoids (88.1-98.6%) are the major constituents of the essential oils. The major constituents of the oils in 9 cultivars of M. arvensis are menthol (73.7-85.8%), menthone (1.5-...

  20. Pb and Cd on growth, leaf ultrastructure and essential oil yield mint (Mentha arvensis L.)

    OpenAIRE

    Caroline Nery Jezler; Pedro Antônio Oliveira Mangabeira; Alex-Alan Furtado de Almeida; Raildo Mota de Jesus; Rosilene Aparecida de Oliveira; Delmira da Costa Silva; Larissa Correa do Bomfim Costa

    2015-01-01

    Contamination of medicinal plants with heavy metals as Pb and Cd can affect the growth and the essential oil production of the plants and represent a risk to those who consume as medicine. This study aimed to evaluate the effects of absorption and localization of Pb and Cd on growth, ultrastructural aspects of leaves and essential oil yield and composition of Mentha arvensis, applied on the soil with increasing concentrations (8, 16, 32, 64 and 128mg kg-1). There was a differential absorption...

  1. Development of mint (Mentha piperita L.) grown on biosolids: evaluation of productivity and essential oil content

    OpenAIRE

    Joseane Scavroni; Leonardo Cesar Ferreira; Janice Valmorbida; Carmen Sílvia Fernandes Boaro

    2009-01-01

    Development of Mentha piperita L . on biosolids amended soil with levels equivalent to 0, 28, 56 and 112 t ha-1 was evaluated. In order to measure the productivity and its relation with mint essential oil yield, different indices were determined: leaf area, total and several organ dry matter, leaf area ratio, specific leaf area, net assimilation rate and relative growth rate at 30, 44, 58, 72 and 86 days after planting (DAP), and essential oil yield at 90, 110 and 120 DAP. Physiological indic...

  2. Chemical composition and antibacterial activity of the essential oil from Mentha requienii Bentham.

    Science.gov (United States)

    Chessa, Mario; Sias, Angela; Piana, Andrea; Mangano, Giuseppe Salvatore; Petretto, Giacomo Luigi; Masia, Maria Dolores; Tirillini, Bruno; Pintore, Giorgio

    2013-01-01

    The chemical composition of essential oil obtained by hydrodistillation of the fresh aerial parts of Mentha requienii Bentham (Lamiaceae) collected on the Gennargentu Mountains (Sardinia, Italy) has been investigated by gas chromatography and gas chromatography-mass spectrometry. The main constituents that resulted were pulegone (78%), menthone (0.5%), isomenthone (18%), isopulegone (1.3%) and limonene (1.76%). In vitro antifungal activity is evaluated in order to identify new means that could be helpful in the prevention of contamination in indoor environments.

  3. Mentha pulegium extract as a natural product for the inhibition of corrosion. Part I: electrochemical studies.

    Science.gov (United States)

    Khadraoui, Abdelkader; Khelifa, Abdellah; Boutoumi, Hocine; Hammouti, Belkheir

    2014-01-01

    The inhibitory effect of Mentha pulegium extract (MPE) on steel corrosion in 1 M HCl solution was investigated using potentiodynamic polarisation and electrochemical impedance spectroscopy. The inhibition efficiency of MPE was found to increase with the concentration and reached 88% at 33% (v/v). Polarisation measurements show that the natural extract acted as a mixed inhibitor. The remarkable inhibition efficiency of MPE was discussed in terms of blocking of electrode surface by adsorption of inhibitor molecules through active centres. The adsorption of MPE was found to accord with the Temkin isotherm.

  4. [Sex structure and seed productivity of Mentha canadensis L. from natural flora of primorye of Russia].

    Science.gov (United States)

    Voronkova, T V; Shelepova, O V; Kondrat'eva, V V; Bidiukova, G F

    2014-01-01

    The sex structure and seed productivity of Mentha canadensis L. from different climatic regioins of Primorye of Russia was studied. We established that M. canadensis is characterized by a homogeneous population structure due to the formation of vegetative clones. The ratio of female and androgynous individuals was 1:5, and it is possible that this is a species-specific trait. Both sexual forms produced fruits under conditions of isolation from cross-pollination. We discuss the possibility of apomixis and the influence of climatic conditions on seed productivity and morphometric characteristics of plants.

  5. Identification of EST–SSRs and molecular diversity analysis in Mentha piperita

    OpenAIRE

    Birendra Kumar; Umesh Kumar; Hemant Kumar Yadav

    2015-01-01

    EST sequences of Mentha piperita available in the public domain (NCBI) were exploited to develop SSR markers. A total of 1316 ESTs were assembled into 155 contigs and 653 singletons and of these, 110 sequences were found to contain 130 SSRs, with a frequency of 1 SSR/3.4 kb. Dinucleotide repeat SSRs were most frequent (72.3%) with the AG/CT (43.8%) repeat motif followed by AT/AT (16.2%). Primers were successfully designed for 68 SSR-containing sequences (62.0%). The 68 primers amplified 13 ac...

  6. 不同薄荷品种遗传关系的ISSR分析%ISSR Analysis of Genetic Relationships between Eleven Varieties of Mentha

    Institute of Scientific and Technical Information of China (English)

    乐云辰; 吴亚妮; 张艳玲; 姚雷

    2008-01-01

    本研究利用ISSR分子标记技术对上海地区适应性种植良好的11个薄荷品种的遗传关系进行了分析.从分子水平阐明其遗传多样性和相似性,以期完善薄荷品种资源的评价方法.结果显示,12个引物共检测到176个位点,其中多态位点170个,多态位点百分率为96.59%.Nei遗传多样性指数平均为0.2280,平均遗传相似系数为0.50,分布在0.19到0.67之间.聚类分析结果显示,圆叶留兰香(Mentha rotundifolia)与留兰香(Mentha spicata L.)成一组,巧克力薄荷(Mentha piperita L. cv.chocolate)、黑胡椒薄荷(Mentha piperita f.rubfscens)、胡椒薄荷(Mentha piperita L)、薰衣草薄荷(Mentha piperita cv.lavandula)和柠檬辣薄荷(Menthapiperita var.citrata)成一组,苹果薄荷(Mentha suoveolens)、菠萝薄荷(Metha suaveolens cv.varie gata)与葡萄柚薄荷(Mentha suave-olens ×piperita)成一组,唇萼薄荷(Mentha pulegium L)单独成一组.该分析结果与以前根据形态进行的分类结果一致.

  7. Pentacyclic triterpenoids of Mentha villosa: structural identification and {sup 1}H and {sup 13}C resonance assignments; Triterpenoides pentaciclicos de Mentha villosa: identificacao estrutural e atribuicao dos deslocamentos quimicos dos atomos de hidrogenio e carbono

    Energy Technology Data Exchange (ETDEWEB)

    Monte, Francisco J. Queiroz; Oliveira, Eliete F. de [Ceara Univ., Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica; Braz Filho, Raimundo [Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ (Brazil). Setor de Quimica de Produtos Naturais

    2001-08-01

    The structures of seven oleanene and ursene triterpenoids (1-7) isolated from aerial parts of Mentha villosa were identified. In addition, the complete {sup 1} H and {sup 13} C resonance assignments of these triterpenoids were accomplished using 1D and 2D NMR spectroscopic experiments. (author)

  8. Anti-yeast activity of mentha oil and vapours through in vitro and in vivo (real fruit juices) assays.

    Science.gov (United States)

    Tyagi, Amit Kumar; Gottardi, Davide; Malik, Anushree; Guerzoni, Maria Elisabetta

    2013-04-15

    The anti-yeast activity of mentha oil and vapours was evaluated against 8 food spoiling yeasts through disc diffusion, disc volatilisation and micro broth dilution method. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) varied from 0.28 to 2.25 and 1.13 to 4.5 mg/ml, respectively. Furthermore, the anti-yeast efficacy of mentha oil alone and in combination with thermal treatment was evaluated in a real food system i.e. mixed fruit juices. The samples treated with a combination of mentha oil at the MIC, ½ MIC and ¼ MIC levels and thermal treatment enhanced the reduction viability. Chemical characterisation of mentha oil by gas chromatography-mass spectrometry (GC-MS) revealed that the dominant compounds were cis-menthone (27.43%), menthol (24.3%), trans-menthone (9.23%), limonene (5.84%), menthofuran (4.44%) and isomenthol (3.21%). Present results established the superior performance of integrated treatment over individual exposure for fruit juice preservation.

  9. IMPACT OF ANISE (PIMPINELLA ANISUM AND MINT (MENTHA PIPERITA ESSENTIAL OILS TO MICROBIAL ACTIVITY IN CHICKEN MEAT

    Directory of Open Access Journals (Sweden)

    Miroslava Kačániová

    2015-02-01

    Full Text Available The goal of the present study was to evaluate fresh chicken thighs quality (microbiological and sensory after treatment by ethylenediaminetetraacetate (EDTA, Pimpinella anisum L. and Mentha piperita essential oils in 1% concentration, stored under vacuum packaging (VP, at 4±0.5°C for a period of 16 days. The following treatments of chicken thighs were applied: air-packaged (AC, control samples, vacuum-packaged (VPC, control samples, vacuum-packed with EDTA solution 1.50% v/w (VPEC, control samples, VP with Pimpinella anisum L. and Mentha piperita essential oil at concentrations 0.1% v/w (VP+AEO and VP+MEO. The quality assessment of VP product after EDTA treatment, Pimpinella anisum L. and Mentha piperita oils was done by microbiological testing and the total viable counts, Enterobacteriaceae, lactic acid bacteria and Pseudomonas aeruginosa were detected. The using of Pimpinella anisum L. and Mentha piperita oils and EDTA with combination of vacuum packaging has significant effect (P < 0.05 to reduction of microorganisms compared with control group without vacuum packaging and untreated control group.

  10. A Comparative Study of Three Cryopreservation Protocols for Effective Storage of In Vitro-Grown Mint (Mentha Spp.)

    Science.gov (United States)

    This study was designed to determine the response of diverse mint genotypes to three commonly used cryopreservation techniques. Four mints [Mentha x piperita nothosubsp. citrata (Ehrh.) Briq.; M. canadensis L.; M. australis R. Br, and M. cunninghamii Benth] were cryopreserved using the three standa...

  11. Gaschromatografisch onderzoek naar de samenstelling van de vluchtige olie van Mentha piperita L. gedurende de ontwikkeling van de plant

    NARCIS (Netherlands)

    Malingré, Theodorus Maria

    1966-01-01

    SAMENVATTING Het doel van het beschreven onderzoek was door middel van gaschromatografie, gecombineerd met andere analysemethoden, de samenstelling van de vluchtige olie van Mentha piperita L. en de veranderingen hierin gedurende de ontwikkeling van de plant, te bepalen en uit de resultaten van het

  12. EFFECT OF DIETARY LEVELS OF SPEARMINT (Mentha spicata ON BROILER CHICKS PERFORMANCE

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    E.O. AMASAIB

    2013-07-01

    Full Text Available This study was conducted to determine the effect of addition different levels of spearmint (Mentha spicata on broiler chick’s performance. One hundred and twenty eight day old unsexed (Cobb broiler chicks were used in this experiment. Birds were distributed randomly into 16 pens (8/pen as replicates, in a complete randomized design. The experimental diets were formulated with four levels of spearmint (Mentha spicata of 0, 1, 1.5 and 2%. Feed and water were freely accessed. Feed intake, body weight gain and feed conversion ratio were weekly recorded and Mortality rate was recorded throughout the experiment. At the end of the experimental period, four birds from each treatment were randomly selected, weighed and slaughtered for determination of carcass weight and dressing percentage. Average feed intakes obtained from the experiment were 2680.20, 2679.11, 2708.55 and 2692.57 for diets 0, 1, 1.5%and 2%, respectively. However, the body weight gains for the treatments were 1481.63, 1512.81, 1519.57 and 1519.63, 0, 1, 1.5 and 2, respectively. Feed conversion ratios for treatments were found to be 1.92, 1.94, 1.92 and 1.99 respectively. Dressing percentage were 73.12, 74.17, 73.08 and73.47 respectively. The results indicated that the supplementation of different levels of spearmint to the diets of broiler improved feed intake and body weight gain.

  13. Chemical composition, antimicrobial and antioxidant properties of Mentha longifolia (L. Huds. essential oil

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    Haris Nikšić

    2012-12-01

    Full Text Available Introduction: Present study describes the antimicrobial activity and free radical scavenging capacity (RSC of essential oil from Mentha longifolia (L. Huds. Aim of this study to investigate the quality, antimicrobial andantioxidant activity of wild species Mentha longifolia essential oil from Bosnia and Herzegovina.Methods: The chemical profi le of essential oil was evaluated by the means of gas chromatography-mass spectrometry (GC-MS and thin-layer chromatography (TLC. Antimicrobial activity was tested against 6bacterial strains. RSC was assessed by measuring the scavenging activity of essential oils on 2,2- diphenyl-1-picrylhydrazil (DPPH.Results: The main constituents of the essential oil of M. longifoliae folium were oxygenated monoterpenes,piperitone oxide (63.58% and 1,8-cineole (12.03%. Essential oil exhibited very strong antibacterial activity.The most important antibacterial activity essential oil was expressed on Gram negative strains: Escherichia coli, Pseudomonas aerginosa and Salmonella enterica. subsp.enterica serotype ABONY. Antioxidant activity was evaluated as a RSC. Investigated essential oil was able to reduce DPPH radicals into the neutral DPPHH form (IC50=10.5 μg/ml and this activity was dose –dependent.Conclusion: The study revealed signifi cant antimicrobial activity of the investigated essential oil. The examined oil exhibited high RSC, which was found to be in correlation to the content of mainly monoterpeneketones and aldehydes. These results indicate that essential oils could serve as safe antioxidant and antiseptic supplements in pharmaceuticals.

  14. Identification and quantification of phenolics in Australian native mint (Mentha australis R. Br.).

    Science.gov (United States)

    Tang, Kitty S C; Konczak, Izabela; Zhao, Jian

    2016-02-01

    Australian native mints have traditionally been used by the aboriginal people for natural remedies; however, their bioactive components have not been studied. Antioxidant capacity and composition of phenolic compounds of Mentha australis R. Br., Lamiaceae were investigated for the first time. Phenolic compounds were analyzed by HPLC photodiode array detector, liquid chromatography high resolution mass spectrometry, tandem mass spectrometry and nuclear magnetic resonance spectroscopy. Aqueous methanolic extract of the mint exhibited comparable antioxidant capacity to the common spearmint. Major compounds identified in the extract were rosmarinic acid (160.4 ± 0.85 μg mg(-1)purified extract), neoponcirin (145.0 ± 0.42 μg gallic acid equivalent(GAE) mg(-1)), narirutin (30.3 ± 0.02 μg GAE mg(-1)), chlorogenic acid (15.4 ± 0.05 μg mg(-1)) and biochanin A (9.6 ± 0.06 μg GAE mg(-1)), while minor compounds were caffeic acid, apigenin, hesperetin and naringenin. Neoponcirin and biochanin A were identified for the first time in the Mentha genus.

  15. Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L.

    Institute of Scientific and Technical Information of China (English)

    Nripendra Nath Biswas; Subarna Saha; Mohammed Khadem Ali

    2014-01-01

    Objective: To investigate potential antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. in different in vivo and in vitro experimental models. Methods: In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the plant extract. In vivo analgesic activity was carried out by acetic acid-induced writhing test in Swiss albino mice. All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight. Antibacterial activity was studied by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains. Brine shrimp lethality assay was used to investigate cytotoxicity effects of the plant extract. Results:The extract showed free radical scavenging activity in the DPPH assay (IC50~41 µg/mL) compared to the standard antioxidant ascorbic acid (IC50~19 µg/mL). The extract also produced prominent antimicrobial activity against Salmonella typhi, Salmonella paratyphi, Shigella boydii,Streptococcus pyogenes and Streptococcus aureus compared to standard drug kanamycin at the dose of 30 µg/disc. The extract exhibited lethality against the brine shrimp nauplii with the LC50 values of 40 µg/mL, and also 90% mortality (LC90) value was found to be 160 µg/mL. In analgesic test, the extract demonstrated statistically significant (P Conclusions:These results suggest that the ethanolic extract of Mentha arvensis L. has potential antioxidant, antibacterial, cytotoxic and analgesic activities that support the ethnopharmacological uses of this plant.

  16. Identification and quantification of phenolics in Australian native mint (Mentha australis R. Br.).

    Science.gov (United States)

    Tang, Kitty S C; Konczak, Izabela; Zhao, Jian

    2016-02-01

    Australian native mints have traditionally been used by the aboriginal people for natural remedies; however, their bioactive components have not been studied. Antioxidant capacity and composition of phenolic compounds of Mentha australis R. Br., Lamiaceae were investigated for the first time. Phenolic compounds were analyzed by HPLC photodiode array detector, liquid chromatography high resolution mass spectrometry, tandem mass spectrometry and nuclear magnetic resonance spectroscopy. Aqueous methanolic extract of the mint exhibited comparable antioxidant capacity to the common spearmint. Major compounds identified in the extract were rosmarinic acid (160.4 ± 0.85 μg mg(-1)purified extract), neoponcirin (145.0 ± 0.42 μg gallic acid equivalent(GAE) mg(-1)), narirutin (30.3 ± 0.02 μg GAE mg(-1)), chlorogenic acid (15.4 ± 0.05 μg mg(-1)) and biochanin A (9.6 ± 0.06 μg GAE mg(-1)), while minor compounds were caffeic acid, apigenin, hesperetin and naringenin. Neoponcirin and biochanin A were identified for the first time in the Mentha genus. PMID:26304400

  17. In-vitro Antioxidant Activity and GC/MS Studies on the Leaves of Mentha piperita (Lamiaceae from Morocco

    Directory of Open Access Journals (Sweden)

    Elhoussine derwich

    2011-04-01

    Full Text Available The present study was to evaluate the in vitro antioxidant activity and chemical composition of essential oil of Mentha piperita from Morocco. Mentha species from the Lamiaceae family are widely distributed in Morocco and commonly used as herbal tea, flavoring agent and medicinal plant. In this stud y, the essential oils of Mentha piperita collected in the region of Meknes (Morocco were obtained by hydro-distillation of the leaves and analysed by gas chromatography equipped with flame ionisation detector (GC-FID and gas chromatography coupled to mass spectrometry system (GC/MS for their chemical composition. The antioxidant activity of essential oils against DPPH radical was determined in vitro by treated with different concentrations of essential oil and vitamin C as standard antioxidant compound. The percentages of DPPH inhibition and IC50 were recorded. Thirty compounds were identified in leaves oil representing 58.61% of the total oil composition. The yield of essential oil of Mentha piperita was 1.02% and the major compound in the leaves was: Menthone (29.01% followed by menthol (5.58%, menthyl acetate (3.34%, menthofuran (3.01%, 1,8-cineole (2.40%, isomenthone (2.12%, limonene (2.10%, α-pinene (1.56%, germacrene-D (1.50%, β-pinene (1.25%, sabinene (1.13% and pulegone (1.12%. The radical scavenging activity (% inhibition of the essential oil from Mentha piperita was the highest (81.09± 1.21% at the concentration of 150µg/ml and the IC50 values of these plant extracts were 53.67µg/ml.

  18. Physiological indexese macro- and micronutrients in plant tissue and essential oil of Mentha piperita L. grown in nutrient solution with variation in N, P, K and Mg levels

    OpenAIRE

    E.F.S. David; M. M. MISCHAN; M.O.M. Marques; C.S.F. Boaro

    2014-01-01

    Mentha piperita L. is an aromatic and medicinal species of the family Lamiaceae, known as mint or peppermint, and its leaves and branches produce essential oil rich in menthol. This study aimed to evaluate physiological indexes, macro- and micronutrients inthe shootsand essential oil of Mentha piperita L. grown in nutrient solution number 2 of Hoagland and Arnon (1950) with different N, P, K and Mg levels. Shoot length, dry mass of the different organs, total dry mass, leaf area, essential oi...

  19. Mentha spicata var. spicata (L. 1753) et Raphanus sativus var. sativus (L. 1753) : repas de survie pour Medauroidea extradentata (Brunner von Wattenwyl 1907) (Phasmatodea: Phasmatidae)

    OpenAIRE

    Olive, Gabriel; Zimmer, Jean-Yves; OLIVE, Gilles

    2016-01-01

    The English version of this preprint can be found at hal-01270314 . Mentha spicata var. spicata (L. 1753) and Raphanus sativus var. sativus (L. 1753): survival meal for Medauroidea extradentata (Brunner von Wattenwyl 1907) (Phasmatodea: Phasmatidae). When food shortages for a stick insect breeding of the species Medauroidea extradentata, feeding trials were conducted with spearmint (Mentha spicata var. spicata). Contrary to what was expected, the stems were eaten more than the leaves. In a...

  20. Constituintes voláteis de Mentha pulegium L. e Plectranthus amboinicus (Lour. Spreng Volatile constituents of Mentha pulegium L. and Plectranthus amboinicus (Lour. Spreng

    Directory of Open Access Journals (Sweden)

    R.A Oliveira

    2011-01-01

    Full Text Available Nesse trabalho foi investigado a composição química de óleos essenciais de duas plantas medicinais cultivadas em Ilhéus, Brasil, Plectranthus amboinicus (Lour. Spreng. (hortelã-grosso e Mentha pulegium L. (poejo. Os óleos essenciais foram extraídos das folhas frescas por hidrodestilação e foram analisados por CG/FID e CG-EM, na primavera e no inverno. P. amboinicus forneceu, tanto na primavera como no inverno, 0,10% de óleo sendo timol o componente majoritário. M. pulegium forneceu na primavera 0,20% de óleo e no inverno 0,09%. Os componentes majoritários dos óleos foram pulegona e trans-cariofileno; borneol, mentol e piperitona foram identificados em menores quantidades. No inverno foram observados maiores quantidades de mentol e isomentol. Acetatos de neoisomentila, de mentila e de isometila foram observados somente no inverno. Esse trabalho contribui para o conhecimento das espécies vegetais cultivadas na região do sul da BahiaThis work investigated the chemical composition of the essential oils of two medicinal plants grown in Ilhéus Municipality, Bahia State, Brazil: Plectranthus amboinicus (Lour. Spreng (Mexican mint and Mentha pulegium L. (pennyroyal. The essential oils were extracted from fresh leaves by hydrodistillation and analyzed by GC-FID and GC-MS, in the spring and in the winter. In both seasons, P. amboinicus yielded 0.10% oil and had thymol as major component. M. pulegium yielded 0.20% and 0.09% oil in the spring and winter, respectively. The major components were pulegone and trans-caryophyllene, whereas borneol, menthol and piperitone were identified at small quantities. Higher menthol and isomenthol levels were detected in the winter. In addition, neoisomenthyl, menthyl and isomenthyl acetates were only observed in the winter. This work contributes to the knowledge of plant species grown in southern Bahia

  1. Essential oil of Origanum majorana L., Illicium verum Hook. f. and Cinnamomum zeylanicum Blume: chemical and antimicrobial characterization Óleos essenciais de Origanum majorana L., Illicium verum Hook. f. e Cinnamomum zeylanicum Blume: caracterização química e antimicrobiana

    Directory of Open Access Journals (Sweden)

    J.M Freire

    2011-01-01

    Full Text Available Essential oils of Origanum majorana L. (marjoram, Illicium verum Hook. f. (star-anise and Cinnamomum zeylanicum Blume (cinnamon were obtained by steam distillation using a modified Clevenger device. The antimicrobial activity of each oil was evaluated against the bacteria Staphylococcus aureus, Escherichia coli and the fungi Aspergillus flavus and Aspergillus parasiticus by observing their growth and/or mycelial inhibition through comparison with the standard dish (without oil. The essential oils were analyzed using a gas chromatograph coupled to a mass spectrometer for identification and coupled to a flame ionization detector for quantification. The major constituents of marjoram, star-anise and cinnamon essential oils were 4-terpineol, trans-anetole and cinnamic aldehyde, respectively. In in vitro tests, essential oils of marjoram and cinnamon promoted an inhibitory effect on the bacteria S. aureus and E. coli, while the essential oil of star-anise presented activity only against E. coli. Marjoram, star-anise and cinnamon oils were effective against the studied fungi, presenting an inhibitory effect. The minimal inhibitory concentration for the mycelial growth of A. parasiticus was 1 and 0.01 µL mL-1 for star-anise and cinnamon oils, respectively. The minimal inhibitory concentration for A. parasiticus was 0.25, 2 and 2 µL mL-1 for cinnamon, star-anise and marjoram oils, respectively.Óleos essenciais de Origanum majorana L. (manjerona, Illicium verum Hook. f. (anis estrelado e Cinnamomum zeylanicum Blume (canela foram obtidos pela técnica de arraste a vapor d'água com aparelho de Clevenger modificado. Foram avaliadas as atividades antimicrobianas de cada um sobre as bactérias Staphylococcus aureus, Escherichia coli e para os fungos Aspergillus flavus e Aspergillus parasiticus, observando o crescimento e/ou inibição micelial, comparando-se estes com a placa-padrão (sem óleo. Os óleos essenciais foram analisados em cromat

  2. Essential Oil Composition of Menthol Mint (Mentha arvensis and Peppermint (Mentha piperita Cultivars at Different Stages of Plant Growth from Kumaon Region of Western Himalaya

    Directory of Open Access Journals (Sweden)

    R.S. Verma

    2010-01-01

    Full Text Available Menthol mint (Mentha arvensis L. and peppermint (M. piperita L. cultivars grown in Kumaon region were evaluated for essential oil content and composition at different stages of crop growth. In men­thol mint cultivars viz., ‘Kosi’, ‘Saksham’, ‘Himalaya’, and ‘Kalka’, the essential oil content was found to vary from 0.3% - 1.2%, 0.42% - 1.1%, 0.38% - 1.0% and 0.26% - 1.2%, at different days after transplanting (DAT respectively, while in cultivars ‘Kukrail’, ‘CIM-Madhurus’ and ‘CIM-Indus’ of peppermint, it varied from 0.28% - 0.6%, 0.19% - 0.55% and 0.17% - 0.37%, respectively at different DAT. The menthol content in all the menthol mint cultivars reached higher values at 120 and 150 DAT. In case of peppermint cultivars viz., Kukrail’, ‘CIM-Madhurus’ and ‘CIM-Indus’, menthol content varied from 32.92% - 39.65%, 34.29% - 42.83% and 22.56% - 32.77%, respectively during the crop growth.

  3. ANTIMICROBIAL ACTIVITY OF MENTHA ARVENSIS AGAINST CLINICAL ISOLATES OF HUMAN CARIOGENIC PATHOGENS- AN IN-VITRO STUDY

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    Vinod Singh et al.

    2012-05-01

    Full Text Available Patients with chronic dental infection are usually treated with antibiotics. However, the value of antibiotics was decreasing because increased resistance in bacteria. The objective of this study is to evaluate the efficacy of herbal crude extract of Mentha arvensis in human Cariogenic pathogens. In this study we obtained crud extract of Mentha arvensis in different solvent 50% and 10% methanol, ethyl acetate, chloroform and was tested against human Cariogenic pathogens Streptococcus mutans, Streptococcus sangunis, Staphylococcus aurues, Lactobacillus casei were isolated from patients having dental disease. The crude extracts activity were studied by disc diffusion and both dilution methods in different concentration. Studies were also undertaken to assess the phytochemical composition of the Mentha arvensis extract. 50% methanolic extract at 2.5mg/ml and 5mg/ml concentration shows slightly bigher zone of inhibition (ranging from 26 to 30 mm and 28 to 32 mm, and 10% methanolic 2.5mg/ml and 5mg/ml extract shows slightly small zone (ranging from 20 to 24 mm and 22 to 27 mm and comparison with ethyl acetate and chloroform shows small zone at 5mg/ml ranging from 15 to 18 mm and 13 to 17 mm and in 2.5gm/ml ranging from 14 to 15mm and 09 to 16 mm or to be moderately sensitive. MIC results exhibit the profound and promising activity of Mentha arvensis on BHI 0.090 mg/ml. The secondary metabolites commonly present in the test leaves are Alkaloids, Tannins, Flavonols, Steroids, Xantones and glycosides, The GCMS analysis of revealed, the presence of Eucalyptol, Isomethone, Linalool, methnol, 4-Terpineol, OleicAcid, Tetradecanoic acid, 12-methyl-, methyl ester, Hexadecanoic acid, (Palmitic acid methyl ester. These data suggest that extracts of Mentha arvensis contain significant amounts of phytochemicals with antioxidative properties which could serve antimicrobial property of the Mentha arvensis and it is exploited as a potential source for plant

  4. Effects of enhanced UV-B radiation on Mentha spicata essential oils

    International Nuclear Information System (INIS)

    In vitro propagated plantlets representing two distinct chemotypes of Mentha spicata, viz. plants producing essential oils rich in piperitone oxide and piperitenone oxide (chemotype I) and rich in carvone and dihydrocarvone (chemotype II), were grown in the field under ambient or ambient plus supplemental UV-B radiation, biologically equivalent to a 15% ozone depletion over Patras (38.3°N, 29.1°E), Greece. Enhanced UV-B radiation stimulated essential oil production in chemotype II substantially, while a similar, non-significant trend was observed in chemotype I. No effect was found on the qualitative composition of the essential oils, whereas the quantitative composition was slightly modified in chemotype I. This is the first investigation reporting an improved essential oil content under UV-B supplementation in aromatic plants under field conditions

  5. 椒样薄荷的栽培技术%Culture of Mentha piperital

    Institute of Scientific and Technical Information of China (English)

    冯晓侠

    2005-01-01

    椒样薄荷学名Mentha piperital(唇形科、薄荷属),别名黑薄荷、胡椒薄荷、欧洲薄荷。原产欧洲及地中海沿岸一带,1959年从国外引进,目前国内在陕西、四川等地有少量栽培。从其茎叶中提炼的精油广泛应用于食品、化妆品、医药卫生等方面,因而种植薄荷的前景十分广阔。

  6. Anatomical, morphological, and phytochemical effects of inoculation with plant growth- promoting rhizobacteria on peppermint (Mentha piperita).

    Science.gov (United States)

    del Rosario Cappellari, Lorena; Santoro, Maricel Valeria; Reinoso, Herminda; Travaglia, Claudia; Giordano, Walter; Banchio, Erika

    2015-02-01

    Plant growth-promoting rhizobacteria (PGPR) generally exert their effects through enhancement of plant nutrient status and/or phytohormone production. The effects of PGPR on aromatic plant species are poorly known. We measured plant growth parameters, chlorophyll content, trichome density, stomatal density, and levels of secondary metabolites in peppermint (Mentha piperita) seedlings inoculated with PGPR strains Bacillus subtilis GB03, Pseudomonas fluorescens WCS417r, P. putida SJ04, or a combination of WCS417r + SJ04. The treated plants, in comparison with controls, showed increases in shoot biomass, root biomass, leaf area, node number, trichome density, and stomatal density, and marked qualitative and quantitative changes in monoterpene content. Improved knowledge of the factors that control or affect biosynthesis of secondary metabolites and monoterpene accumulation will lead to strategies for improved cultivation and productivity of aromatic plants and other agricultural crops without the use of chemical fertilizers or pesticides.

  7. Effect of different drying methods on the essential oils of mint (Mentha haplocalyx).

    Science.gov (United States)

    Shu, Yachun; Chen, Yajun; Qin, Kunming; Cai, Hao; Wu, Li; Li, Huan; Xu, Lijuan; Caib, Baochang

    2013-10-01

    The essential oils of fresh, shade-dried, sun-dried, and oven-dried mint of Mentha haplocalyx Brig., and the shade-dried herbs after one hour of soaking were analyzed by GC-MS to provide a scientific basis to regulate the drying methods. Fifty-nine compounds were isolated and identified, including 35 from fresh herbs, 25 from shade-dried herbs, 23 from sun-dried herbs, 17 from oven-dried herbs and 48 from shade-dried mint after one hour of soaking. Eighteen compounds were common to all five samples, including menthol, menthone, and isomenthone, which were the main components. Several of these significantly decreased in shade-dried mint soaked in water. Thus in cleaning and drying processes soaking mint in water should be avoided as far as possible, in case major components are extracted thus producing an inferior product that will undermine its curative effect.

  8. Meat quality characteristics in Japanese quails fed with Mentha piperita plant

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    Babak Aminzade

    2012-06-01

    Full Text Available In current study, the effect of different levels of Mentha piperita plant (MPP on meat quality characteristics in Japanese quail wasinvestigated. 180 quails were carried out in completely randomized design with three levels of MPP (0, 1.5 and 3 percentage. Four replicateswith 15 quails were allocated to each experimental treatment. At the end of experiment, after slaughter and evisceration two birds from eachreplicate of the treatment, the carcasses of the birds were kept in a refrigerator (2-40С for 24 hours. Meat quality parameters including thebrightness, yellowness and redness of colors, water holding capacity (WHC, acidity (pH, thiobarbituric acid-reacting substances (TBARSand intramuscular fat (IMF were performed on breast meat samples. The results showed that yellowness, redness and thiobarbituric acid-reactingsubstances (TBARS affected by diets containing MPP (p<0.05. There was no significantly difference for the rest of meat quality traitssuch as brightness, WHC, pH and IMF.

  9. Larvicidal activity of Mentha x villosa Hudson essential oil, rotundifolone and derivatives.

    Science.gov (United States)

    Lima, Tamires Cardoso; da Silva, Tayane Kayne Mariano; Silva, Fabiana Lima; Barbosa-Filho, José Maria; Marques, Márcia Ortiz Mayo; Santos, Roseli La Corte; Cavalcanti, Sócrates Cabral de Holanda; de Sousa, Damião Pergentino

    2014-06-01

    The aim of this study was to evaluate the larvicidal activity of Mentha x villosa essential oil (MVEO) and its major constituent, rotundifolone, against larvae of Aedes aegypti. Additionally, a set of 15 analogues of the rotundifolone were evaluated to identify the molecular characteristics which contribute to the larvicidal effect. The results from the present study showed that the MVEO exhibited outstanding toxic effects against Ae. aegypti larvae (LC50=45.0ppm). Rotundifolone exhibited reasonable larvicidal activity (LC50=62.5ppm). With respect to comparative study of rotundifolone and its analogues, all tested compounds were less potent than rotundifolone, except (-)-limonene. In general, replacement of C-C double bonds by epoxides groups decreases the larvicidal potency. The presence of α,β-unsaturated carbonyls contributes to the larvicidal toxicity. The addition of hydroxyl groups in the chemical structure resulted in less potent compounds. Furthermore, the enantioselectivity seems to play an important role for the larvicidal toxicity.

  10. Assessment of vacuum-dried peppermint (Mentha piperita L.) as a source of natural antioxidants.

    Science.gov (United States)

    Uribe, Elsa; Marín, Daniela; Vega-Gálvez, Antonio; Quispe-Fuentes, Issis; Rodríguez, Angela

    2016-01-01

    The purpose of this study was to investigate the effect of temperature in the vacuum drying process of Mentha piperita L. (50 to 90 °C). Generally, drying processes affect the quality of product, however, vacuum drying works under sub-atmospheric pressures. In order to investigate how temperature affects this herb, as to color, chlorophyll, total phenolic (TPC), total flavonoid (TFC) contents, antioxidant activity by DPPH and ORAC methods. Mineral, vitamin C and sugar contents were also evaluated. A slight change in color and chlorophyll content was observed in the samples. The highest values for TPC, TFC and antioxidant activity methods were obtained at 50 and 70 °C however, a decrease in the vitamin C content was observed. Minerals such as K, Ca, Mg and Na were found. Sucrose showed the highest sugar content. This work suggests that M. piperita L. can be used as a natural antioxidant, whether fresh or dried.

  11. Assessment of vacuum-dried peppermint (Mentha piperita L.) as a source of natural antioxidants.

    Science.gov (United States)

    Uribe, Elsa; Marín, Daniela; Vega-Gálvez, Antonio; Quispe-Fuentes, Issis; Rodríguez, Angela

    2016-01-01

    The purpose of this study was to investigate the effect of temperature in the vacuum drying process of Mentha piperita L. (50 to 90 °C). Generally, drying processes affect the quality of product, however, vacuum drying works under sub-atmospheric pressures. In order to investigate how temperature affects this herb, as to color, chlorophyll, total phenolic (TPC), total flavonoid (TFC) contents, antioxidant activity by DPPH and ORAC methods. Mineral, vitamin C and sugar contents were also evaluated. A slight change in color and chlorophyll content was observed in the samples. The highest values for TPC, TFC and antioxidant activity methods were obtained at 50 and 70 °C however, a decrease in the vitamin C content was observed. Minerals such as K, Ca, Mg and Na were found. Sucrose showed the highest sugar content. This work suggests that M. piperita L. can be used as a natural antioxidant, whether fresh or dried. PMID:26213010

  12. Metabolic Profile of Offspring from Diabetic Wistar Rats Treated with Mentha piperita (Peppermint

    Directory of Open Access Journals (Sweden)

    Sandra M. Barbalho

    2011-01-01

    Full Text Available This study aimed at evaluating glycemia and lipid profile of offspring from diabetic Wistar rats treated with Mentha piperita (peppermint juice. Male offspring from nondiabetic dams (control group: 10 animals treated with water and 10 treated with peppermint juice and from dams with streptozotocin-induced severe diabetes (diabetic group: 10 animals treated with water and 10 treated with peppermint juice were used. They were treated during 30 days, and, after the treatment period, levels of glycemia, triglycerides, total cholesterol, and fractions were analyzed in the adult phase. The offspring from diabetic dams treated with peppermint showed significantly reduced levels of glucose, cholesterol, LDL-c, and triglycerides and significant increase in HDL-c levels. The use of the M. piperita juice has potential as culturally appropriate strategy to aid in the prevention of DM, dyslipidemia, and its complications.

  13. Preparation and characterization of PEG-Mentha oil nanoparticles for housefly control.

    Science.gov (United States)

    Kumar, Peeyush; Mishra, Sapna; Malik, Anushree; Satya, Santosh

    2014-04-01

    Nanoparticles of Mentha × piperita essential oil were prepared by melt-dispersion method. The nanoparticles prepared at varying oil doses (5-10%, w/v) showed an encapsulation efficiency of 78.2-83.4%, while the oil load was observed to range between 3.64 and 7.46%. The average particle size of the nanoparticles varied between 226 and 331 nm, while polydispersity index showed variation between 0.547 and 1.000. DSC analysis indicated endothermic reaction during formation of nanoparticles, while a 2-term exponential kinetic model was followed during oil release. Nanoparticles showed considerable mortality against housefly larvae in lab (100%) as well as simulated field condition after first week (93%) and 6th week (57%) of application. This was the first study utilizing controlled release property of nanoparticles to formulate a cost effective product for breeding site application against housefly.

  14. An ascomycetous endophyte isolated from Mentha piperita L.: biological features and molecular studies.

    Science.gov (United States)

    Mucciarelli, Marco; Scannerini, Silvano; Bertea, Cinzia M; Maffei, Massimo

    2002-01-01

    A hyaline sterile fungus forming epiphyllous mycelial nets was isolated from meristem cultures of Mentha piperita. Histological studies indicated that the culture isolate is able to colonize stems and leaves with no damage to the host plant. In vitro-grown peppermint plants displayed enhanced vegetative growth when infected by the fungus, with mycelium extending from green tissues to growing rootlets. The production of very thin hyphae growing away from host meristems and the asymptomatic nature of the symbiosis were commonly observed in cultures, where the isolate never sporulated. No attribution to a precise morphospecies was therefore possible and the fungal culture was named sterile mycelium PGP-HSF. Through comparison of the 18 S rDNA sequence of the epibiont to those available in literature and in GenBank we were able to determine that the mutualist of peppermint is a member of the Pyrenomycetes, belonging to the subclass Sordariomycetidae. PMID:21156475

  15. Antioxidant,antimicrobial,cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L.

    Institute of Scientific and Technical Information of China (English)

    Nripendra; Nath; Biswas; Subarna; Saha; Mohammed; khadem; Ali

    2014-01-01

    Objective:To investigate potential antioxidant,antimicrobial,cytotoxic and analgesic activities of ethanolic extract of Mentha arrensis l.,in different in vivo and in vitro experimental models.Methods:In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the plant extract.In vivo analgesic activity was carried out by acetic acid—induced writhing test in Swiss albino mice.All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight.Antibacterial activilv was studied by disk diffusion assay against some Gram—positive and Gram—negative bacterial strains.Brine shrimp lethality assay was used to investigate cyloloxicity effects of the plant extract.Results:The extract showed free radical scavenging activity in the DPPH assay(IC5041 μg/mL)compared to the standard antioxidant ascorbic acid(IC5019 μg/mL).The extract also produced prominent antimicrobial activity against Salmonella typhi.Salmonella paratyphi.Shigella boydii,Streptococcus pyogenes and Streptococcus aureus compared to standard drug kanamycin at the dose of 30 μg/disc.The extract exhibited lethality against the brine shrimp nauplii with the LC50,values of 40 μg/mL.and also 90%mortality(LC90) value was found to be 160 μg/mL.In analgesic test.the extract demonstrated statistically significant(P<0.01) analgesic effect in acetic acid induced writhing in white albino mice al both dose levels.Conclusions:These results suggest that the ethanolic extract of Mentha arvenns L.has potential antioxidant,antibacterial,cytotoxic and analgesic activities that support the ethnopharmacological uses of this plant.

  16. Growth and development of Mentha piperita L. in nutrient solution as affected by rates of potassium

    Directory of Open Access Journals (Sweden)

    Janice Valmorbida

    2007-05-01

    Full Text Available The influence of potassium on the development of Mentha piperita L. was evaluated in the plants grown in nutritive solution modified by variations in potassium, at 6.0, 3.0, and 1.5 mmol L-1, establishing differences between treatments and corresponding, respectively, to the concentration proposed in Hoagland and Arnon's no. 2 solution and reductions by 50% and 75%. Until 21 days after transplanting, the concentration used was diluted to 50% in the three treatments. Evaluations consisted of leaf area, development of the above-ground part, and dry matter of different plant organs. Plants grown with 1.5/3.0 mmol L-1 K showed greater development of the above-ground part. Other variables were not different among plants in the different treatments.A influência do potássio no desenvolvimento de Mentha piperita L. foi avaliada em plantas cultivadas em solução nutritiva modificada pela variação de potássio, com 6.0, 3.0 e 1.5 mmol L-1, que estabeleceram as diferenças entre tratamentos e corresponderam, respectivamente, a sua concentração proposta na solução nº 2 de Hoagland e Arnon e diminuição em 50% e 75%. Até os 21 dias após o transplante a concentração utilizada foi diluída em 50% nos três tratamentos. Avaliou-se área foliar, desenvolvimento da parte aérea e matéria secas dos diferentes órgãos. Plantas cultivadas com 1.5/3.0 mmol L-1 K apresentaram maior desenvolvimento da parte aérea. Demais variáveis não diferiram entre as plantas dos diferentes tratamentos.

  17. Pharmacological Action of Mentha piperita on Lipid Profile in Fructose-Fed Rats.

    Science.gov (United States)

    Mani Badal, Rajendra; Badal, Divya; Badal, Pourush; Khare, Ashish; Shrivastava, Jyoti; Kumar, Vinod

    2011-01-01

    Cardiovascular diseases with an incidence of approximately 50% are the main causes of death in most advanced countries and an increasing trend in the developing world as well. The World Health Organization estimates that 12 million people per year worldwide die from cardiovascular diseases. Cardiovascular diseases are becoming an increasing problem worldwide and hypercholesterolemia has been correlated for coronary heart diseases. Nearly all lipoproteins are involved in this process including cholesterol carried by very low density lipoproteins (VLDL), remnant lipoproteins and low density lipoproteins (LDL). Currently, available hypolipidemic drugs have been associated with the number of side effects. Herbal treatment for hyperlipidemia poses no side effects and is relatively cheap and locally available. In view of this, the present study was carried out to investigate the effect of Menthe piperita on serum lipid levels of albino rats. Mentha piperita aqueous extract (100 mg/Kg, 250 mg/Kg p.o. daily) was fed for 3 weeks on fructose-fed rats and the levels of glucose, cholesterol, triglycerides, very low density lipoprotein, low density lipoprotein, and atherogenic index was measured. Twenty-four male Sprague Dawley rats were divided into four groups (6 per group). The results of present study indicate that Mentha piperita had significant beneficial effects against fructose-induced hyperlipidemia and showed good antioxidant activity. The aqueous extract of the plant produced a significant decrease (p < 0.05) in elevated levels of glucose, cholesterol, triglycerides, very low density lipoprotein, low density lipoprotein and atherogenic index and also increased the high density lipoprotein cholesterol levels and HDL-ratio without affecting serum insulin levels in fructose-fed rats. PMID:24250421

  18. Preliminary study of the antimicrobial activity of Mentha x villosa Hudson essential oil, rotundifolone and its analogues Estudo preliminar da atividade antimicrobiana do óleo essencial de Mentha x villosa Hudson, rotundifolona e seus análogos

    OpenAIRE

    Thúlio A. Arruda; Rossana M.P. Antunes; Raissa M.R. Catão; Lima, Edeltrudes de O.; Damião P. De Sousa; Xirley P. Nunes; Maria S.V. Pereira; José M. Barbosa-Filho; Emidio V. L. da Cunha

    2006-01-01

    Essential oils present antimicrobial activity against a variety of bacteria and yeasts, including species resistant to antibiotics and antifungicals. In this context, this work aims at the evaluation of the antimicrobial activity of the essential oil of Mentha x villosa Hudson ("hortelã da folha miúda"), its major component (rotundifolone) and four similar analogues of rotundifolone (limonene oxide, pulegone oxide, carvone epoxide and (+)-pulegone) against strain standards of Staphylococcus a...

  19. Organization of monoterpene biosynthesis in Mentha. Immunocytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase.

    Science.gov (United States)

    Turner, Glenn W; Croteau, Rodney

    2004-12-01

    We present immunocytochemical localizations of four enzymes involved in p-menthane monoterpene biosynthesis in mint: the large and small subunits of peppermint (Mentha x piperita) geranyl diphosphate synthase, spearmint (Mentha spicata) (-)-(4S)-limonene-6-hydroxylase, peppermint (-)-trans-isopiperitenol dehydrogenase, and peppermint (+)-pulegone reductase. All were localized to the secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development. Immunogold labeling of geranyl diphosphate synthase occurred within secretory cell leucoplasts, (-)-4S-limonene-6-hydroxylase labeling was associated with gland cell endoplasmic reticulum, (-)-trans-isopiperitenol dehydrogenase labeling was restricted to secretory cell mitochondria, while (+)-pulegone reductase labeling occurred only in secretory cell cytoplasm. We discuss this pathway compartmentalization in relation to possible mechanisms for the intracellular movement of monoterpene metabolites, and for monoterpene secretion into the extracellular essential oil storage cavity.

  20. ANTI – INFLAMMATORY AND SEDATIVE – HYPNOTIC ACTIVITY OF THE METHANOLIC EXTRACT OF THE LEAVES OF MENTHA ARVENSIS

    OpenAIRE

    Verma, S. M.; Arora, H.; R. Dubey

    2003-01-01

    Mentha arvensis Linn, a plant used as traditional medicine and in perfumery, has now been explored for its pharmacological activities as an anti-inflammatory and also as sedativehypnotic plant drug. The methanolic extract of the leaves after being processed, was taken for the pharmacological study. Anti-inflammatory activity was carried out on albino rats. Further, the activity was compared to that of a standard anti-inflammatory drug – nimesulide and the percent inhibition of oedema determin...

  1. Effects of salinity stress on some yield parameters and morphological characteristics of spearmint (Mentha spicata L.) in hydroponic conditions

    OpenAIRE

    Z. Safari Mohamadiyeh; Moghaddam, M.; B. Abedy; Samiei, L.

    2015-01-01

    Mint is an important medicinal herb that is cultivated in different parts of the world. In order to investigate the effects of salinity stress on morphological characteristics of spearmint (Mentha spicata L.), an experiment was conducted in 2013, based on complete randomized design, under hydroponic conditions, at Research Greenhouse of Faculty of Agriculture, Ferdowsi University of Mashhad, Iran. Treatments were five salinity levels (0, 30, 60, 90 and 120 mM of sodium chloride) with four rep...

  2. Chemical Composition and In Vitro Antibacterial Activity of Mentha spicata Essential Oil against Common Food-Borne Pathogenic Bacteria

    OpenAIRE

    Yasser Shahbazi

    2015-01-01

    The aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of Mentha spicata plant against common food-borne pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli O157:H7). Chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (GC-MS). The antibacterial activity of the...

  3. Next generation sequencing unravels the biosynthetic ability of Spearmint (Mentha spicata) peltate glandular trichomes through comparative transcriptomics

    OpenAIRE

    Jin, Jingjing; Panicker, Deepa; Wang, Qian; Kim, Mi Jung; Liu, Jun; Yin, Jun-Lin; Wong, Limsoon; Jang, In-Cheol; Chua, Nam-Hai; Sarojam, Rajani

    2014-01-01

    Background Plant glandular trichomes are chemical factories with specialized metabolic capabilities to produce diverse compounds. Aromatic mint plants produce valuable essential oil in specialised glandular trichomes known as peltate glandular trichomes (PGT). Here, we performed next generation transcriptome sequencing of different tissues of Mentha spicata (spearmint) to identify differentially expressed transcripts specific to PGT. Our results provide a comprehensive overview of PGT’s dynam...

  4. Protection against LPS-induced cartilage inflammation and degradation provided by a biological extract of Mentha spicata

    OpenAIRE

    Kott Laima S; Fletcher Ronald S; Pearson Wendy; Hurtig Mark B

    2010-01-01

    Abstract Background A variety of mint [Mentha spicata] has been bred which over-expresses Rosmarinic acid (RA) by approximately 20-fold. RA has demonstrated significant anti-inflammatory activity in vitro and in small rodents; thus it was hypothesized that this plant would demonstrate significant anti-inflammatory activity in vitro. The objectives of this study were: a) to develop an in vitro extraction procedure which mimics digestion and hepatic metabolism, b) to compare anti-inflammatory p...

  5. The yield and essential oil content of mint (Mentha ssp.) in Northern Ostrobothnia

    OpenAIRE

    Aflatuni, A. (Abbas)

    2005-01-01

    Abstract Peppermint (Mentha x piperita L.) oil is one of the most popular and widely used essential oils, mostly because of its main components menthol and menthone. Peppermint oil is used for flavouring pharmaceuticals and oral preparations. Corn mint is the richest source of natural menthol. Carvone-scented mint plants, such as spearmint (M. spicata), are rich in carvone and are widely used as spices, and they are cultivated in several countries. Studies were made into the yield and esse...

  6. the variability of secondary metabolites in mentha × piperita cv . ‘perpeta’ during the development of inflorescence

    OpenAIRE

    Fialová S.; Tekeľová D.; Švajdlenka E.; Potúček P.; Jakubová K.; Grančai D.

    2014-01-01

    Mentha × piperita (L.) Huds. of the family Lamiaceae is a very important species for commercial exploitation due to the high content of essential oil. Besides the essential oils, there are also other significant secondary metabolites in peppermint, especially flavonoids and hydroxycinnamic derivatives. In this study, we evaluate the variability of essential oil constituents and of phenolic compounds in the leaves of M. × piperita during the development of inflorescence. Similar studi...

  7. Development and essential oil yield of mint (Mentha x piperita L.) cultivated in nutritive solution under different levels of phosphorus

    OpenAIRE

    Evelize de Fátima Saraiva David; Carmen Silvia Fernandes Boaro; Martha M. Mischan

    2007-01-01

    To evaluate the development and essential oil yield of Mentha x piperita L, plants were cultivated using complete Hoagland and Arnon (1950) no 2 solution with a 50% increase or decrease in the phosphorus (P) concentration. The following were evaluated: Leaf number, leaf area, stem length, dry mass of several organs, total dry mass, leaf area ratio (LAR), leaf mass ratio (RMF), net assimilatory rate (NAR), relative growth rate (RGR), specific leaf area (SLA), dry mass distribution, and essenti...

  8. A comparison Comparison between analgesic effects of aqueous ethanolic extract of mentha longifolia and morphine in male rats

    OpenAIRE

    Ezatollah Paknia; Mohammad Ebrahim Rezvani; Mohammad Hossain Dashti-Rahmatabadi; Majid Bagheri

    2013-01-01

    Background and Aim: Long-term consumption of many drugs followed by reduction of their effectiveness has necessitated performing research on new analgesics .Thus, the present study was conducted to evaluate the analgesic effects of mentha longifolia and morphine in mice using writhing and hot plate tests. Materials and Methods: In this experimental study, 70 male rats were divided into 7 equal groups. The groups included the control, three experimental groups receiving 400, 800, or 1600 m...

  9. Extraction, Preliminary Characterization and Evaluation of in Vitro Antitumor and Antioxidant Activities of Polysaccharides from Mentha piperita

    OpenAIRE

    Xin Liu; Zhen-Liang Sun; Ai-Rong Jia; Ya-Ping Shi; Rui-Hong Li; Pei-Ming Yang

    2014-01-01

    This study describes the extraction, preliminary characterization and evaluation of the in vitro antitumor and antioxidant activities of polysaccharides extracted from Mentha piperita (MPP). The optimal parameters for the extraction of MPP were obtained by Box-Behnken experimental design and response surface methodology (RSM) at the ratio of water to raw material of 20, extraction time of 1.5 h and extraction temperature at 80 °C. Chemical composition analysis showed that MPP was mainly comp...

  10. Comparative Chemical Analysis of Mentha piperita and M. spicata and a Fast Assessment of Commercial Peppermint Teas.

    Science.gov (United States)

    Buleandra, Mihaela; Oprea, Eliza; Popa, Dana Elena; David, Iulia Gabriela; Moldovan, Zenovia; Mihai, Iuliana; Badea, Irinel Adriana

    2016-04-01

    The hydrodistilled essential oils and volatile compounds (by static headspaces technique) of Mentha piperita L. and M spicata L. were characterized by GC-MS. Headspace analysis of Mentha piperita revealed the existence of menthone (25.4%), 1,8-cineole (17.7%) and menthol (12.1%) as the main components, while the essential oil contained high amounts of menthol (46.8%) and menthone (25.6%). By contrast, headspace analysis of M. spicata showed a high content of limonene (37.0%) together with carvone (13.0%), β-pinene (10.4%) and α-pinene (9.8%), while the essential oil was reach in carvone (51.7%), dihydrocarveol (11.5%) and cis-dihydrocarvone (9.1%). Eleven samples of peppermint tea available on the Romanian market were analysed by headspace GC-MS. The volatile profile of the tea samples was compared with that of Mentha piperita L. and certain differences were emphasized and discussed. PMID:27396216

  11. Isolation of a flavonoid, apigenin 7-O-glucoside, from Mentha longifolia (L.) Hudson subspecies longifolia and its genotoxic potency.

    Science.gov (United States)

    Gulluce, Medine; Orhan, Furkan; Yanmis, Derya; Arasoglu, Tulin; Guvenalp, Zuhal; Demirezer, Lutfiye Omur

    2015-09-01

    Mentha is a medicinal and aromatic plant belonging to the Lamiaceae family, which is widely used in food, flavor, cosmetic and pharmaceutical industries. Recently, it has been found that the use of Mentha as a pharmaceutical source is based on its phytochemical constituents that have far been identified as tannins, saponins, phenolic acids and flavonoids. This study was designed to evaluate the mutagenic and antimutagenic activities of apigenin 7-O-glucoside (A7G), a flavonoid isolated from Mentha longifolia (L.) Hudson subspecies longifolia (ML). The possible antimutagenic potential of A7G was examined against mutagens ethyl methanesulfonate and acridine in an eukaryotic cell system Saccharomyces cerevisiae and sodium azide in Salmonella typhimurium TA1535 and 9-aminoacridine in S. typhimurium TA1537. According to our findings, any concentrations of the A7G used did not show mutagenic activity but exerted strong antimutagenic activities at tested concentrations. The inhibition rates for the Ames test ranged from 27.2% (S. typhimurium TA1535: 0.4 μM/plate) to 91.1% (S. typhimurium TA1537: 0.2 μM/plate) and for the yeast deletion assay from 4% to 57.7%. This genotoxicological study suggests that a flavonoid from ML owing to antimutagenic properties is of great pharmacological importance and might be beneficial to industries producing food additives, cosmetics and pharmaceuticals products.

  12. Comparative Chemical Analysis of Mentha piperita and M. spicata and a Fast Assessment of Commercial Peppermint Teas.

    Science.gov (United States)

    Buleandra, Mihaela; Oprea, Eliza; Popa, Dana Elena; David, Iulia Gabriela; Moldovan, Zenovia; Mihai, Iuliana; Badea, Irinel Adriana

    2016-04-01

    The hydrodistilled essential oils and volatile compounds (by static headspaces technique) of Mentha piperita L. and M spicata L. were characterized by GC-MS. Headspace analysis of Mentha piperita revealed the existence of menthone (25.4%), 1,8-cineole (17.7%) and menthol (12.1%) as the main components, while the essential oil contained high amounts of menthol (46.8%) and menthone (25.6%). By contrast, headspace analysis of M. spicata showed a high content of limonene (37.0%) together with carvone (13.0%), β-pinene (10.4%) and α-pinene (9.8%), while the essential oil was reach in carvone (51.7%), dihydrocarveol (11.5%) and cis-dihydrocarvone (9.1%). Eleven samples of peppermint tea available on the Romanian market were analysed by headspace GC-MS. The volatile profile of the tea samples was compared with that of Mentha piperita L. and certain differences were emphasized and discussed.

  13. Isolation of a flavonoid, apigenin 7-O-glucoside, from Mentha longifolia (L.) Hudson subspecies longifolia and its genotoxic potency.

    Science.gov (United States)

    Gulluce, Medine; Orhan, Furkan; Yanmis, Derya; Arasoglu, Tulin; Guvenalp, Zuhal; Demirezer, Lutfiye Omur

    2015-09-01

    Mentha is a medicinal and aromatic plant belonging to the Lamiaceae family, which is widely used in food, flavor, cosmetic and pharmaceutical industries. Recently, it has been found that the use of Mentha as a pharmaceutical source is based on its phytochemical constituents that have far been identified as tannins, saponins, phenolic acids and flavonoids. This study was designed to evaluate the mutagenic and antimutagenic activities of apigenin 7-O-glucoside (A7G), a flavonoid isolated from Mentha longifolia (L.) Hudson subspecies longifolia (ML). The possible antimutagenic potential of A7G was examined against mutagens ethyl methanesulfonate and acridine in an eukaryotic cell system Saccharomyces cerevisiae and sodium azide in Salmonella typhimurium TA1535 and 9-aminoacridine in S. typhimurium TA1537. According to our findings, any concentrations of the A7G used did not show mutagenic activity but exerted strong antimutagenic activities at tested concentrations. The inhibition rates for the Ames test ranged from 27.2% (S. typhimurium TA1535: 0.4 μM/plate) to 91.1% (S. typhimurium TA1537: 0.2 μM/plate) and for the yeast deletion assay from 4% to 57.7%. This genotoxicological study suggests that a flavonoid from ML owing to antimutagenic properties is of great pharmacological importance and might be beneficial to industries producing food additives, cosmetics and pharmaceuticals products. PMID:23377117

  14. Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species.

    Science.gov (United States)

    Govindarajan, M; Sivakumar, R; Rajeswari, M; Yogalakshmi, K

    2012-05-01

    Mosquitoes are blood-feeding insects and serve as the most important vectors for spreading human diseases such as malaria, yellow fever, dengue fever, and filariasis. The continued use of synthetic insecticides has resulted in resistance in mosquitoes. Synthetic insecticides are toxic and affect the environment by contaminating soil, water, and air, and then natural products may be an alternative to synthetic insecticides because they are effective, biodegradable, eco-friendly, and safe to environment. Botanical origin may serve as suitable alternative biocontrol techniques in the future. Mentha spicata, an edible and medicinal plant, is chiefly distributed in Southeast Asia and South Asia. In the present study, the toxicity of mosquito larvicidal activity of leaf essential oil (EO) and their major chemical constituents from Mentha spicata against Culex quinquefasciatus, Aedes aegypti, and Anopheles stephensi. The chemical composition of the leaf EO was analyzed using gas chromatography-mass spectroscopy (GC-MS). GC-MS revealed that the EO of M. spicata contained 18 compounds. The major chemical components identified were carvone (48.60%), cis-carveol (21.30%), and limonene (11.30%). The EO had a significant toxic effect against early third-stage larvae of C. quinquefasciatus, A. aegypti, and A. stephensi with LC(50) values of 62.62, 56.08, and 49.71 ppm and LC(90) values of 118.70, 110.28, and 100.99 ppm, respectively. The three major pure constituents extracted from the M. spicata leaf EO were also tested individually against three mosquito larvae. The LC(50) values of carvone, cis-carveol, and limonene appeared to be most effective against A. stephensi (LC(50) 19.33, 28.50, and 8.83 ppm) followed by A. aegypti (LC(50) 23.69, 32.88, and 12.01 ppm), and C. quinquefasciatus (LC(50) 25.47, 35.20, and 14.07 ppm). The results could be useful in search for newer, safer, and more effective natural larvicidal agents against C. quinquefasciatus, A. aegypti, and A

  15. Development of mint (Mentha piperita L. grown on biosolids: evaluation of productivity and essential oil content

    Directory of Open Access Journals (Sweden)

    Joseane Scavroni

    2009-04-01

    Full Text Available Development of Mentha piperita L . on biosolids amended soil with levels equivalent to 0, 28, 56 and 112 t ha-1 was evaluated. In order to measure the productivity and its relation with mint essential oil yield, different indices were determined: leaf area, total and several organ dry matter, leaf area ratio, specific leaf area, net assimilation rate and relative growth rate at 30, 44, 58, 72 and 86 days after planting (DAP, and essential oil yield at 90, 110 and 120 DAP. Physiological indices revealed that biosolids prolonged the vegetative phase of the plants, which adapted themselves to the presence of biosolids with time. Plants showed inverse behaviors in relation to productivity, resulting from the primary metabolism, represented by the shoot dry matter yield, and oil yield, resulting from the secondary metabolism. Adaptation of the mint plants to the growth on biosolids could be due to a phytoremediation function of this species. The intrinsic mechanisms of these processes could be better understood in a further evaluation of residual effects in mint plant shoots.Níveis de biossólido equivalentes a 0, 28, 56 e 112 t ha-1 foram avaliados no desenvolvimento de Mentha piperita L. Determinaram-se área foliar e matéria seca total e dos diferentes órgãos, os índices fisiológicos razão de área foliar, área foliar específica, taxa assimilatória líquida e taxa de crescimento relativo, aos 30, 44, 58, 72 e 86 dias após plantio (DAP e o rendimento de óleo essencial aos 90, 110 e 120 DAP. Os índices fisiológicos revelaram que o biossólido prolongou a fase vegetativa das plantas, que se adaptaram com o tempo e apresentaram comportamentos inversos em relação à produtividade, resultado de seu metabolismo primário e representada pela produção de matéria seca da parte aérea e produção de óleo, resultado do metabolismo secundário. A referida adaptação das plantas de menta à presença do biossólido pode ser devido à fun

  16. Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species.

    Science.gov (United States)

    Govindarajan, M; Sivakumar, R; Rajeswari, M; Yogalakshmi, K

    2012-05-01

    Mosquitoes are blood-feeding insects and serve as the most important vectors for spreading human diseases such as malaria, yellow fever, dengue fever, and filariasis. The continued use of synthetic insecticides has resulted in resistance in mosquitoes. Synthetic insecticides are toxic and affect the environment by contaminating soil, water, and air, and then natural products may be an alternative to synthetic insecticides because they are effective, biodegradable, eco-friendly, and safe to environment. Botanical origin may serve as suitable alternative biocontrol techniques in the future. Mentha spicata, an edible and medicinal plant, is chiefly distributed in Southeast Asia and South Asia. In the present study, the toxicity of mosquito larvicidal activity of leaf essential oil (EO) and their major chemical constituents from Mentha spicata against Culex quinquefasciatus, Aedes aegypti, and Anopheles stephensi. The chemical composition of the leaf EO was analyzed using gas chromatography-mass spectroscopy (GC-MS). GC-MS revealed that the EO of M. spicata contained 18 compounds. The major chemical components identified were carvone (48.60%), cis-carveol (21.30%), and limonene (11.30%). The EO had a significant toxic effect against early third-stage larvae of C. quinquefasciatus, A. aegypti, and A. stephensi with LC(50) values of 62.62, 56.08, and 49.71 ppm and LC(90) values of 118.70, 110.28, and 100.99 ppm, respectively. The three major pure constituents extracted from the M. spicata leaf EO were also tested individually against three mosquito larvae. The LC(50) values of carvone, cis-carveol, and limonene appeared to be most effective against A. stephensi (LC(50) 19.33, 28.50, and 8.83 ppm) followed by A. aegypti (LC(50) 23.69, 32.88, and 12.01 ppm), and C. quinquefasciatus (LC(50) 25.47, 35.20, and 14.07 ppm). The results could be useful in search for newer, safer, and more effective natural larvicidal agents against C. quinquefasciatus, A. aegypti, and A

  17. 薄荷化学成分及采收加工研究概况%Research Situation of Chemical Components and Collecting Processing of Menthae Haplocalycis

    Institute of Scientific and Technical Information of China (English)

    束雅春; 徐丽娟; 蔡皓; 蔡宝昌

    2013-01-01

    Menthae Haplocalycis is one of the commonly used clinical pungent-cool diaphoretics.In order to offer the basis of exploiting Menthae Haplocalycis medicinal resources and rational clinical application,the research situation of chemical components and collecting processing of Menthae Haplocalycis in recent years was summarized in this paper.%薄荷是中医临床常用辛凉解表药之一,就近年来薄荷的化学成分及采收加工的研究进展进行综述,为薄荷药材资源的开发及临床合理应用提供依据.

  18. Effect of clinorotation on in vitro cultured explants of Mentha piperita L.

    Science.gov (United States)

    Paolicchi, Fabio; Mensuali-Sodi, Anna; Tognoni, Franco

    2002-02-14

    An in vitro culture system was used to study the influence of gravity on axillary shoot formation and adventitious root regeneration in Mentha piperita L. The direction of the gravity vector was altered by displacing stem node explants in different orientations. Also, microgravity conditions were simulated by rotating the explants on a horizontal clinostat so that the main axis of nodes was either parallel (Cpa) or perpendicular to the clinostat axis (Ccp and Ccf, centripetally and centrifugally oriented, respectively). Mint nodes were cultured on solidified Linsmaier and Skoog's medium [Physiol. Plant. 18 (1965) 100] adding a filter-sterilized aqueous solution of 2 mg/l benzyladenine (BA) in half of the cultures. The proliferation of axillary shoots as well as adventitious root formation were not affected by altering upright explant orientation. On the contrary clinorotation was able to modify plantlet development. In absence of BA, leaf width was hindered by Cpa treatment and penultimate internode length was enhanced by Ccp. Furthermore, a negative effect of Cpa treatment was observed in root length parameter, while Ccp increased the root number both in absence and in presence of BA. An effect strictly connected to clinorotation in presence of BA was the occurrence of hyperhydricity. Moreover, explants under clinorotation treatments switched their gravitropic response modifying shoot curvature.

  19. Differentiation of mint (Mentha haplocalyx Briq.) from different regions in China using gas and liquid chromatography.

    Science.gov (United States)

    Dong, Wenjiang; Ni, Yongnian; Kokot, Serge

    2015-02-01

    In this study, complex substances such as Mint (Mentha haplocalyx Briq.) samples from different growing regions in China were analyzed for phenolic compounds by high-performance liquid chromatography with diode array detection and for the volatile aroma compounds by gas chromatography with mass spectrometry. Chemometrics methods, e.g. principal component analysis, back-propagation artificial neural networks, and partial least squares discriminant analysis, were applied to resolve complex chromatographic profiles of Mint samples. A total of 49 aroma components and 23 phenolic compounds were identified in 79 Mint samples. Principal component analysis score plots from gas chromatography with mass spectrometry and high-performance liquid chromatography with diode array detection data sets showed a clear distinction among Mint from three different regions in China. Classification results showed that satisfactory performance of prediction ability for back-propagation artificial neural networks and partial least squares discriminant analysis. The major compounds that contributed to the discrimination were chlorogenic acid, unknown 3, kaempherol 7-O-rutinoside, salvianolic acid L, hesperidin, diosmetin, unknown 6 and pebrellin in Mint according to regression coefficients of the partial least squares discriminant analysis model. This study indicated that the proposed strategy could provide a simple and rapid technique to distinguish clearly complex profiles from samples such as Mint.

  20. Chemical diversity in Mentha spicata: antioxidant and potato sprout inhibition activity of its essential oils.

    Science.gov (United States)

    Chauhan, Shailendra S; Prakash, Om; Padalia, Rajendra C; Vivekanand; Pant, Anil K; Mathela, Chandra S

    2011-09-01

    The essential oils from fresh aerial parts of Mentha spicata L. collected from ten different natural habitats of Uttarakhand, India were analyzed by a combination of GC, GC/MS and NMR spectroscopy. The analysis revealed that monoterpenoids (46.1%-91.6%), mainly carvone (15.3%-68.5%), piperetenone oxide (24.0%-79.2%) and alpha-humulene (0.1%-29.9%), were the major constituents of the essential oils, but with significant qualitative and quantitative differences among the other constituents. Cluster analysis of the oil composition was carried out in order to discern the differences and similarities within different accessions collected from different natural habitats. The essential oils were also screened for their antioxidant activities by chelating properties of Fe2+, DPPH radical-scavenging activity, and their reducing power. The essential oils of two chemo variants (viz. carvone and piperetenone oxide types) were also tested for potato (Solanum tuberosum L.) sprout suppressant activity. The results showed that these oils exhibit good sprout inhibition activity in comparison to CIPC and iodine, the standard sprout suppressant. PMID:21941918

  1. Biochemical and histochemical localization of monoterpene biosynthesis in the glandular trichomes of spearmint (Mentha spicata)

    Energy Technology Data Exchange (ETDEWEB)

    Gershenzon, J.; Maffei, M.; Croteau, R. (Washington State Univ., Pullman (USA))

    1989-04-01

    The primary monoterpene accumulated in the glandular trichomes of spearmint (Mentha spicata) is the ketone (-)-carvone which is formed by cyclization of the C{sub 10} isoprenoid intermediate geranyl pyrophosphate to the olefin (-)-limonene, hydroxylation to (-)-trans-carveol and subsequent dehydrogenation. Selective extraction of the contents of the glandular trichomes indicated that essentially all of the cyclase and hydroxylase activities resided in these structures, whereas only about 30% of the carveol dehydrogenase was located here with the remainder located in the rest of the leaf. This distribution of carveol dehydrogenase activity was confirmed by histochemical methods. Electrophoretic analysis of the partially purified carveol dehydrogenase from extracts of both the glands and the leaves following gland removal indicated the presence of a unique carveol dehydrogenase species in the glandular trichomes, suggesting that the other dehydrogenase found throughout the leaf probably utilizes carveol only as an adventitious substrate. These results demonstrate that carvone biosynthesis takes place exclusively in the glandular trichomes in which this natural product accumulates.

  2. Proteomic snapshot of spearmint (Mentha spicata L.) leaf trichomes: a genuine terpenoid factory.

    Science.gov (United States)

    Champagne, Antoine; Boutry, Marc

    2013-11-01

    Peltate glandular trichomes from Mentha spicata were purified on a Percoll gradient and soluble and membrane proteins were trypsinized and the peptides were separated by nano-LC fractionation and analyzed by MALDI-MS/MS. The vast majority of the 1666 proteins identified were housekeeping proteins or involved in the primary metabolism. However, 57 were predicted to be involved in the secondary metabolism. Of these, 21 were involved in the synthesis of phenylpropanoids and phenolics and 32 in terpenoid synthesis. Of the 14 membrane transporters identified, the 11 ATP-binding cassette transporters provide good material for assessing whether active transport is required for the transfer of monoterpenoid intermediates between cellular compartments and for the secretion of the final products into the subcuticular storage cavity. In conclusion, this proteome analysis of M. spicata peltate trichomes has identified several candidate proteins that might be involved in terpenoid synthesis and transport. The data have been deposited to the ProteomeXchange with identifier PXD000352 (http://proteomecentral.proteomexchange.org/dataset/PXD000352). PMID:24124164

  3. Protective effects of bioactive phytochemicals from Mentha piperita with multiple health potentials

    Directory of Open Access Journals (Sweden)

    Seyedeh Maryam Sharafi

    2010-01-01

    Full Text Available Mentha piperita essential oil was bactericidal in order of E. coli> S. aureus > Pseudomonas aeruginosa> S. faecalis > Klebsiella pneumoniae. The oil with total phenolics of 89.43 ± 0.58 μg GAE/mg had 63.82 ± 0.05% DPPH inhibition activity with an IC 50 = 3.9 μg/ml. Lipid peroxidation inhibition was comparable to BHT and BHA. A 127% hike was noted in serum ferric-reducing antioxidant power. There was 38.3% decrease in WBCs count, while platelet count showed increased levels of 214.12%. Significant decrease in uric acid level and cholesterol/HDL and LDL/HDL ratios were recorded. The volatile oil displayed high cytotoxic action toward the human tumor cell line. The results of this study deserve attention with regard to antioxidative and possible anti-neoplastic chemotherapy that form a basis for future research. The essential oil of mint may be exploited as a natural source of bioactive phytopchemicals bearing antimicrobial and antioxidant potentials that could be supplemented for both nutritional purposes and preservation of foods.

  4. Mentha piperita in nephrotoxicity - a possible intervention to ameliorate renal derangements associated with gentamicin

    Directory of Open Access Journals (Sweden)

    Naveed Ullah

    2014-01-01

    Full Text Available Objective: Free radical generation has a strong role in the pathogenesis of renal damage associated with the use of gentamicin. Therefore, the present study was carried out to evaluate the renoprotective effect of Mentha piperita against gentamicin induced nephrotoxicity. Materials and Methods: A total of 24 male rabbits were divided into 4 groups receiving normal saline, gentamicin, M. piperita extract and co-therapy of extract and gentamicin respectively. Gentamicin was provided as 80 mg/kg/day intramuscularly and extract was given 200 mg/kg/day orally for a period of 21 days. Serum and urinary biochemical parameters and histological changes were studied for each group. The impact of the extract on the antibacterial action of gentamicin was also evaluated. Results: Animals treated with gentamicin showed derangements in serum and urinary biochemical parameters. These alterations were reversed by treatment with M. piperita extract. The histological changes showed in gentamicin group were also reverted by treatment with the extract. Further the plant did not influence the efficacy of gentamicin with respect to its antimicrobial properties. Conclusion: Co-therapy of M. piperita with gentamicin successfully attenuated biochemical kidney functioning derangements and morphological changes associated with gentamicin.

  5. Contact toxicity and repellency of the essential oil from Mentha haplocalyx Briq. against Lasioderma serricorne.

    Science.gov (United States)

    Zhang, Wen-Juan; Yang, Kai; You, Chun-Xue; Wang, Cheng-Fang; Geng, Zhu-Feng; Su, Yang; Wang, Ying; Du, Shu-Shan; Deng, Zhi-Wei

    2015-05-01

    The chemical composition of the essential oil obtained by hydrodistillation from the aerial parts of Mentha haplocalyx was investigated by GC-FID and GC/MS analyses. In sum, 23 components, representing 92.88% of the total oil composition, were identified, and the main compounds were found to be menthol (59.71%), menthyl acetate (7.83%), limonene (6.98%), and menthone (4.44%). By bioassay-guided fractionation (contact toxicity), three compounds were obtained from the essential oil and identified as menthol, menthyl acetate, and limonene. The essential oil and the three isolated compounds exhibited potent contact toxicity against Lasioderma serricorne adults, with LD50 values of 16.5, 7.91, 5.96, and 13.7 μg/adult, respectively. Moreover, the oil and its isolated compounds also exhibited strong repellency against L. serricorne adults. At the lower concentrations tested and at 2 h after exposure, menthol showed even significantly stronger repellency than the positive control DEET. The study revealed that the bioactivity properties of the essential oil can be attributed to the synergistic effects of its diverse major and minor components, which indicates that the M. haplocalyx oil and its isolated compounds have potential for the development as natural insecticides and/or repellents to control insects in stored grains and traditional Chinese medicinal materials.

  6. Water Extract of Mentha x villosa: Phenolic Fingerprint and Effect on Ischemia-Reperfusion Injury.

    Science.gov (United States)

    Fialovaa, Silvia; Veizerova, Lucia; Nosalova, Viera; Drabikova, Katarina; Tekelova, Daniela; Grancai, Daniel; Sotnikova, Ruzena

    2015-06-01

    Qualitative analysis of the water extract of Mentha x villosa Huds. leaves was performed by liquid chromatography mass spectrometry (LC-MS/MS) and quantitative analysis was made by reverse-phase liquid chromatography coupled with photodiode array detection (LC-DAD). Sixteen phenolic compounds were identified and quantified consisting of 8 phenolic acids/derivatives and 8 flavonoid glycosides (quinic acid, chlorogenic acid, coumaroyl-hexoside, caffeic acid, coumaroylquinic acid, lithospermic acid, rosmarinic acid, salvianolic acid A, luteolin-7-O-glucuronide, luteolin-7-O-glucoside, luteolin-7-O-rutinoside, eriodictyol-7-O-rutinoside, apigenin-7-O-glucuronide, kaempferol-3-O-glucuronide, chrysoeriol-7-O-rutinoside, and hesperetin-7-O-rutinoside). Luteolin-7- O-rutinoside (25.6 ± 0.7 mg/g dry extract) and rosmarinic acid (17.9 ± 0.4 mg/g dry extract) were the most abundant. High antioxidant activity of this phenolic-rich water extract was confirmed in vitro by DPPH and ABTS tests and ex vivo in the ischemia-reperfusion injured rat superior mesenteric artery. Thus, the water extract of M. x villosa leaves seems to be a promising agent in prevention of tissue injury caused by oxidative stress.

  7. Coliform bacteria removal from sewage in constructed wetlands planted with Mentha aquatica.

    Science.gov (United States)

    Avelar, Fabiana F; de Matos, Antonio T; de Matos, Mateus P; Borges, Alisson C

    2014-08-01

    The present study evaluated the performance of the species Mentha aquatica in constructed wetlands of horizontal subsurface flow (CW-HSSF) with regard to the removal of coliforms bacteria in an effluent from the primary treatment of sewage as well as to obtain adjustment parameters of the bacterial decay kinetic model along the length of the CW-HSSF. Therefore, four CW-HSSFs measuring 24.0 m x 1.0 m x 0.35 m were built and filled with number 0 gravel as the support medium to a height of 0.20m. Two of the CW-HSSFs were planted with the species M. aquatica, while the other two remained uncultivated. Cultivation of M. aquatica in CW-HSSF resulted in total coliforms (TC) and Escherichia coli (EC) removals from 0.9 to 1.3 log units greater than those obtained in the uncultivated experimental plots, for the hydraulic retention times (HRTs) of 4.5 and 6.0 days. For HRT ranged from 1.5 to 6.0 days, the highest removal efficiencies in counts of TC and EC were obtained when using longer HRT. The mathematical models evaluated showed good fit to average counts of TC and EC highlighting the modified first-order kinetic model with the inclusion of the power parameter in the HRT variable.

  8. Essential oil composition of sixteen elite cultivars of Mentha from western Himalayan region, India

    Directory of Open Access Journals (Sweden)

    Rajendra C. Padalia

    2013-02-01

    Full Text Available The hydrodistilled essential oils of 16 cultivars of Mentha, viz. M. arvensis L., M. spicata L. and M. citrata Ehrh., were analysed and compared by gas chromatography and gas chromatography-mass spectrometry. Fifty-seven constituents representing 92.8-99.8% of the total essential oil composition were identified. Monoterpenoids (88.1-98.6% are the major constituents of the essential oils. The major constituents of the oils in 9 cultivars of M. arvensis are menthol (73.7-85.8%, menthone (1.5-11.0%, menthyl acetate (0.5-5.3%, isomenthone (2.1-3.9%, limonene (1.2-3.3% and neomenthol (1.9-2.5%. Carvone (51.3-65.1%, limonene (15.1-25.2%, -pinene (1.3-3.2% and 1,8-cineole (≤0.1-3.6% are the major constituents in 5 cultivars of M. spicata, while in one cultivar (Ganga of M. spicata the major constituents are piperitenone oxide (76.7%, α-terpineol (4.9% and limonene (4.7%. Linalool (59.7%, linalyl acetate (18.4%, nerol (2.0%, trans-p-menth-1-en-2-ol (1.8%, a-terpineol (1.5% and limonene (1.1% are the major constituents of M. citrata.

  9. Quantitative estimation of pulegone in Mentha longifolia growing in Saudi Arabia. Is it safe to use?

    Science.gov (United States)

    Alam, Prawez; Saleh, Mahmoud Fayez; Abdel-Kader, Maged Saad

    2016-03-01

    Our TLC study of the volatile oil isolated from Mentha longifolia showed a major UV active spot with higher Rf value than menthol. Based on the fact that the components of the oil from same plant differ quantitatively due to environmental conditions, the major spot was isolated using different chromatographic techniques and identified by spectroscopic means as pulegone. The presence of pulegone in M. longifolia, a plant widely used in Saudi Arabia, raised a hot debate due to its known toxicity. The Scientific Committee on Food, Health & Consumer Protection Directorate General, European Commission set a limit for the presence of pulegone in foodstuffs and beverages. In this paper we attempted to determine the exact amount of pulegone in different extracts, volatile oil as well as tea flavoured with M. longifolia (Habak) by densitometric HPTLC validated methods using normal phase (Method I) and reverse phase (Method II) TLC plates. The study indicated that the style of use of Habak in Saudi Arabia resulted in much less amount of pulegone than the allowed limit.

  10. Mentha suaveolens Ehrh. (Lamiaceae) Essential Oil and Its Main Constituent Piperitenone Oxide: Biological Activities and Chemistry.

    Science.gov (United States)

    Božović, Mijat; Pirolli, Adele; Ragno, Rino

    2015-01-01

    Since herbal medicines play an important role in the treatment of a wide range of diseases, there is a growing need for their quality control and standardization. Mentha suaveolens Ehrh. (MS) is an aromatic herb with fruit and a spearmint flavor, used in the Mediterranean areas as a traditional medicine. It has an extensive range of biological activities, including cytotoxic, antimicrobial, antioxidant, anti-inflammatory, hypotensive and insecticidal properties, among others. This study aims to review the scientific findings and research reported to date on MS that prove many of the remarkable various biological actions, effects and some uses of this species as a source of bioactive natural compounds. On the other hand, piperitenone oxide (PO), the major chemical constituent of the carvone pathway MS essential oil, has been reported to exhibit numerous bioactivities in cells and animals. Thus, this integrated overview also surveys and interprets the present knowledge of chemistry and analysis of this oxygenated monoterpene, as well as its beneficial bioactivities. Areas for future research are suggested.

  11. Proteomic snapshot of spearmint (Mentha spicata L.) leaf trichomes: a genuine terpenoid factory.

    Science.gov (United States)

    Champagne, Antoine; Boutry, Marc

    2013-11-01

    Peltate glandular trichomes from Mentha spicata were purified on a Percoll gradient and soluble and membrane proteins were trypsinized and the peptides were separated by nano-LC fractionation and analyzed by MALDI-MS/MS. The vast majority of the 1666 proteins identified were housekeeping proteins or involved in the primary metabolism. However, 57 were predicted to be involved in the secondary metabolism. Of these, 21 were involved in the synthesis of phenylpropanoids and phenolics and 32 in terpenoid synthesis. Of the 14 membrane transporters identified, the 11 ATP-binding cassette transporters provide good material for assessing whether active transport is required for the transfer of monoterpenoid intermediates between cellular compartments and for the secretion of the final products into the subcuticular storage cavity. In conclusion, this proteome analysis of M. spicata peltate trichomes has identified several candidate proteins that might be involved in terpenoid synthesis and transport. The data have been deposited to the ProteomeXchange with identifier PXD000352 (http://proteomecentral.proteomexchange.org/dataset/PXD000352).

  12. Quantitative estimation of pulegone in Mentha longifolia growing in Saudi Arabia. Is it safe to use?

    Science.gov (United States)

    Alam, Prawez; Saleh, Mahmoud Fayez; Abdel-Kader, Maged Saad

    2016-03-01

    Our TLC study of the volatile oil isolated from Mentha longifolia showed a major UV active spot with higher Rf value than menthol. Based on the fact that the components of the oil from same plant differ quantitatively due to environmental conditions, the major spot was isolated using different chromatographic techniques and identified by spectroscopic means as pulegone. The presence of pulegone in M. longifolia, a plant widely used in Saudi Arabia, raised a hot debate due to its known toxicity. The Scientific Committee on Food, Health & Consumer Protection Directorate General, European Commission set a limit for the presence of pulegone in foodstuffs and beverages. In this paper we attempted to determine the exact amount of pulegone in different extracts, volatile oil as well as tea flavoured with M. longifolia (Habak) by densitometric HPTLC validated methods using normal phase (Method I) and reverse phase (Method II) TLC plates. The study indicated that the style of use of Habak in Saudi Arabia resulted in much less amount of pulegone than the allowed limit. PMID:27087088

  13. Growth response and carcass characteristics of Japanese quail to Mentha piperita plant supplementation

    Directory of Open Access Journals (Sweden)

    Babak Aminzade

    2012-06-01

    Full Text Available In current study, the effect of Mentha piperita plant (MPP supplementation on performance and carcass characteristics in Japanesequail was investigated. A total of 180 quails (male and female were carried out in completely randomized design with diet consisted of thebasal diet as the control (0MPP, diets with MPP at 1.5% (15 g/kg MPP and 3% (30 g/kg MPP. Four replicates with 15 quails were allocatedto each experimental treatment and the birds were reared for 42 days. The diets were formulated according to NRC (1994 guidelines and contained24% protein and 2900 kcal/kg ME. The results showed that weight gain, feed intake and feed conversation was significantly affected bylevels of MPP (p<0.05. Quails fed with 1.5% MPP had more percentage of breast muscle compared to quails fed 0 and 3% MPP. Feed conversionratio was significantly improved in quails received 1.5% compared to 3% (p<0.05. There was no significant difference for percentage ofcarcass and thigh muscle among experimental treatments. The result showed adding various levels of MPP was improved feed conversion ratioand increase body weight in Japanese quail.

  14. Metabolism of monoterpenes: evidence for compartmentation of l-menthone metabolism in peppermint (Mentha piperita) leaves

    Energy Technology Data Exchange (ETDEWEB)

    Martinkus, C.; Croteau, R.

    1981-01-01

    Previous studies have shown that the monoterpene ketone l-(G-/sup 3/H)-menthone is reduced to the epimeric alcohols l-menthol and d-neomenthol in leaf discs of flowering peppermint (Mentha piperita L.), and that a portion of the menthol is converted to menthyl acetate while the bulk of the neomenthol is transformed to neomenthyl-..beta..-D-glucoside. When l-(3-/sup 3/H)menthol and d-(3-/sup 3/H)neomenthol are separately administered to leaf discs, both menthyl and neomenthyl acetates and menthyl and neomenthyl glucosides are formed with nearly equal facility, suggesting that the metabolic specificity observed with the ketone precursor was not a function of the specificity of the transglucosylase or transacetylase but rather a result of compartmentation of each stereospecific dehydrogenase with the appropriate transferase. Co-purification of the acceptor-mediated activities, and differential activation and inhibition studies, provided strong evidence that the same UDP-glucose-dependent enzyme could transfer glucose to either l-menthol or d-neometnthol. These results demonstrate that the specific in vivo conversion of l-menthone to l-menthyl acetate and d-neomenthyl-..beta..-D-glucoside cannot be attributed to the selectivity of the transferases, and they clearly indicate that the metabolic specificity observed is a result of compartmentation effects.

  15. Differentiation of mint (Mentha haplocalyx Briq.) from different regions in China using gas and liquid chromatography.

    Science.gov (United States)

    Dong, Wenjiang; Ni, Yongnian; Kokot, Serge

    2015-02-01

    In this study, complex substances such as Mint (Mentha haplocalyx Briq.) samples from different growing regions in China were analyzed for phenolic compounds by high-performance liquid chromatography with diode array detection and for the volatile aroma compounds by gas chromatography with mass spectrometry. Chemometrics methods, e.g. principal component analysis, back-propagation artificial neural networks, and partial least squares discriminant analysis, were applied to resolve complex chromatographic profiles of Mint samples. A total of 49 aroma components and 23 phenolic compounds were identified in 79 Mint samples. Principal component analysis score plots from gas chromatography with mass spectrometry and high-performance liquid chromatography with diode array detection data sets showed a clear distinction among Mint from three different regions in China. Classification results showed that satisfactory performance of prediction ability for back-propagation artificial neural networks and partial least squares discriminant analysis. The major compounds that contributed to the discrimination were chlorogenic acid, unknown 3, kaempherol 7-O-rutinoside, salvianolic acid L, hesperidin, diosmetin, unknown 6 and pebrellin in Mint according to regression coefficients of the partial least squares discriminant analysis model. This study indicated that the proposed strategy could provide a simple and rapid technique to distinguish clearly complex profiles from samples such as Mint. PMID:25431171

  16. The antioxidative effect of Iranian Mentha pulegium extracts and essential oil in sunflower oil.

    Science.gov (United States)

    Kamkar, Abolfazl; Javan, Ashkan Jebelli; Asadi, Farzad; Kamalinejad, Mohammad

    2010-07-01

    The aim of the present study was to evaluate antioxidative activities of the essential oil, methanol and water extracts of Iranian pennyroyal in vegetable oil during storage. Different concentrations (0, 200, 400, 600, 800 and 1000 ppm) of essential oil, water and methanol extracts and beta-hydroxy toluene (BHT; 200 ppm) were added to sunflower oil emulsion in the presence of cupric ions and incubated for 7 days at 60 degrees C. Peroxide values (PVs) and thiobarbituric acid reacting substances (TBARS) levels were measured in each day up to day of seven. Furthermore, antioxidant capacity of the essential oil and extracts were determined using DPPH and beta-carotene-linoleic acid methods. Values were compared among groups in each incubation time points using ANOVA. Results showed that DPPH and beta-carotene-linoleic acid assay findings on the Mentha pulegium extracts were comparable to those found on BHT. Furthermore, in all incubation time points, M. pulegium extracts lowered PVs and TBARS levels when compared to the control (poil did not show considerable antioxidative effect. It seems that water extract of M. pulegium is a potent antioxidant which makes it as a potential antioxidant for oil and oily products during storage.

  17. Chemical composition and biological activities of the essential oil of Mentha suaveolens Ehrh.

    Science.gov (United States)

    El-Kashoury, El-Sayeda A; El-Askary, Hesham I; Kandil, Zeinab A; Salem, Mohamed A; Sleem, Amany A

    2012-01-01

    Hydrodistilled oils of the fresh aerial parts of Mentha suaveolens Ehrh. cultivated in Egypt were prepared from samples collected along the four seasons. The percentage yields of these essential oils were 0.50%, 0.52%, 0.60%, and 0.47% of the dry weight for winter, spring, summer, and autumn samples. GC/MS analyses of all samples revealed a qualitative and quantitative variability in the oil composition. The total number of compounds identified was 46 among which 15 were common in all samples. The oxygenated compounds constituted about 45%, 46%, 63%, and 44% of the total composition of the oils for winter, spring, summer, and autumn samples, respectively. Carvone was the major constituent in spring, summer, and autumn samples (about 31%, 56%, and 35%, respectively), while limonene (ca. 26%) was the major constituent of the winter sample followed by carvone (ca. 25%). The essential oil of the highest yield (full-flowering summer sample), with the highest oxygenated constituents and carvone contents, was screened for certain biological activities. It exhibited analgesic and acute anti-inflammatory activities (75% and 82% relative to indomethacin). It also showed a potent in vivo antioxidant activity (96% relative to vitamin E). In addition, it exerted moderate cytotoxic, hepatoprotective, and in vitro antioxidant activities. Moreover, the oil had a potent antifungal activity against Candida albicans (MIC = 4 microg/ml), Saccharomyces cerevisiae (MIC = 5.2 microg/ml), and Aspergillus niger (MIC = 6.8 microg/ml).

  18. Antioxidant and antimicrobial activities of the essential oil and solvent extracts of Mentha pulegium L.

    Directory of Open Access Journals (Sweden)

    Palić Ivan R.

    2015-01-01

    Full Text Available We report the total phenolic (TPC; expressed as gallic acid equivalents, GAE, per milligram of dry extract weight and the total flavonoid contents (TFC; expressed as quercetin equivalents, QE, per milligram of dry extract weight and antimicrobial and antioxidant activities of the essential oil and hexane, diethyl ether, ethyl acetate and methanol extracts of Mentha pulegium L. (Lamiaceae collected in Serbia. The total phenolic content was in the range of 129.43-388.29 μg GAE/mg, while TFC ranged from 57.81 to 160.94 QE/mg; the highest TPC and TFC were found in the methanol extract. The antimicrobial activity (against five bacteria and two fungi species of the essential oil and solvent extracts was assessed using disc-diffusion method. However, the studied samples demonstrated a poor antimicrobial potential. The antioxidant activity was screened using five different tests: 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay (DPPH, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radical cation decolorization assay (ABTS, total reducing power (TRP, ferric reducing antioxidant power (FRAP and cupric reducing antioxidant capacity assay (CUPRAC; the methanol extract showed the strongest antioxidant potential. The results of the different antioxidant assays were correlated mutually and with the total flavonoid and total phenolic contents (regression analysis and agglomerative hierarchical clustering. [Projekat Ministarstva nauke Republike Srbije, br. 172047

  19. Pb and Cd on growth, leaf ultrastructure and essential oil yield mint (Mentha arvensis L.

    Directory of Open Access Journals (Sweden)

    Caroline Nery Jezler

    2015-03-01

    Full Text Available Contamination of medicinal plants with heavy metals as Pb and Cd can affect the growth and the essential oil production of the plants and represent a risk to those who consume as medicine. This study aimed to evaluate the effects of absorption and localization of Pb and Cd on growth, ultrastructural aspects of leaves and essential oil yield and composition of Mentha arvensis, applied on the soil with increasing concentrations (8, 16, 32, 64 and 128mg kg-1. There was a differential absorption of Pb and Cd by M. arvensis mainly concentrated in the roots. Pb was found in small amounts in the leaves while Cd largely exceeded the safety limit without symptoms of toxicity. The ultrastructural analysis revealed the metal accumulation on vesicles surrounding the mitochondria and the presence of electron dense deposits surrounding the mitochondria, nucleus and chloroplasts. Little changes caused by Pb and Cd application were not enough to affect the growth and essential oil yield and composition of M. arvensis

  20. Radiosensitivity on the components of essential oil in the genus Mentha

    Energy Technology Data Exchange (ETDEWEB)

    Ono, Seiroku (Okayama Univ. (Japan). Faculty of Agriculture)

    1992-10-01

    The effects of seed irradiation using X-rays (20KR) on the components of the essential oil in the adult plant were investigated using Mentha arvensis L. var. piperascens Malinvaud (2n=96). 1. X-ray irradiation produced almost no effect on the dry matter weight and content of essential oil at any stage of growth. 2. Using X-ray irradiation, the level of free menthol was increased, and ester menthol and menthone were decreased. This seemed to suggest that menthol was synthesized by the reduction of the menthone. 3. The content of free menthol was found to increase linearly toward leaves of the upper level, while the content of menthone was found to gradually decrease and, finally, to disappear in leaves at fifth level of leaves. 4. No difference was found in the ratios of contents of inorganic component between the first and second harvest seasons. There was also no difference in the content of total nitrogen at any location of leaves, except when an increased concentration of nitrogen at the seventh level of leaves was found. (author).

  1. Chemical Composition, Antifungal and Antibiofilm Activities of the Essential Oil of Mentha piperita L.

    Science.gov (United States)

    Saharkhiz, Mohammad Jamal; Motamedi, Marjan; Zomorodian, Kamiar; Pakshir, Keyvan; Miri, Ramin; Hemyari, Kimia

    2012-01-01

    Variations in quantity and quality of essential oil (EO) from the aerial parts of cultivated Mentha piperita were determined. The EO of air-dried sample was obtained by a hydrodistillation method and analyzed by a gas chromatography/mass spectrometry (GC/MS). The antifungal activity of the EO was investigated by broth microdilution methods as recommended by Clinical and Laboratory Standards Institute. A biofilm formation inhibition was measured by using an XTT reduction assay. Menthol (53.28%) was the major compound of the EO followed by Menthyl acetate (15.1%) and Menthofuran (11.18%). The EO exhibited strong antifungal activities against the examined fungi at concentrations ranging from 0.12 to 8.0 μL/mL. In addition, the EO inhibited the biofilm formation of Candida albicans and C. dubliniensis at concentrations up to 2 μL/mL. Considering the wide range of the antifungal activities of the examined EO, it might be potentially used in the management of fungal infections or in the extension of the shelf life of food products.

  2. Chemical and morphological diversity in wild populations of Mentha longifolia in Israel.

    Science.gov (United States)

    Segev, Daniel; Nitzan, Nadav; Chaimovitsh, David; Eshel, Amram; Dudai, Nativ

    2012-03-01

    Populations of Mentha longifolia, an endangered species in Israel, were tested for essential oil composition and conservational ability. In 2002-2003, 25 wild populations country-wide were tested, indicating population divergence into two chemotypes. Chemotype A was characterized by high levels of menthone and pulegone, and chemotype B by high levels of piperitenone oxide and piperitone oxide. Chemotype A was more abundant (22 of 25 populations) than chemotype B (11 of 25 populations). However, a chemotype/population interaction was not recorded (P > 0.05). In spring 2003, seven of the 25 wild populations were resampled, propagated, and cultivated at the Newe Ya'ar campus. Then, in 2004, the propagated plants were tested for essential oil composition. The propagated plants maintained the essential oil composition as well as the chemotype-frequency distribution of the original wild population from which they were obtained. Since a chemotype/population interaction was not recorded, and the cultivated plants displayed the wild population essential oil composition, it can be concluded that i) the chemotype diversity is genetically based, and ii) the M. longifolia populations sampled can be horticulturally conserved.

  3. Mentha suaveolens Ehrh. (Lamiaceae Essential Oil and Its Main Constituent Piperitenone Oxide: Biological Activities and Chemistry

    Directory of Open Access Journals (Sweden)

    Mijat Božović

    2015-05-01

    Full Text Available Since herbal medicines play an important role in the treatment of a wide range of diseases, there is a growing need for their quality control and standardization. Mentha suaveolens Ehrh. (MS is an aromatic herb with fruit and a spearmint flavor, used in the Mediterranean areas as a traditional medicine. It has an extensive range of biological activities, including cytotoxic, antimicrobial, antioxidant, anti-inflammatory, hypotensive and insecticidal properties, among others. This study aims to review the scientific findings and research reported to date on MS that prove many of the remarkable various biological actions, effects and some uses of this species as a source of bioactive natural compounds. On the other hand, piperitenone oxide (PO, the major chemical constituent of the carvone pathway MS essential oil, has been reported to exhibit numerous bioactivities in cells and animals. Thus, this integrated overview also surveys and interprets the present knowledge of chemistry and analysis of this oxygenated monoterpene, as well as its beneficial bioactivities. Areas for future research are suggested.

  4. Effects of Mentha suaveolens Essential Oil Alone or in Combination with Other Drugs in Candida albicans.

    Science.gov (United States)

    Stringaro, Annarita; Vavala, Elisabetta; Colone, Marisa; Pepi, Federico; Mignogna, Giuseppina; Garzoli, Stefania; Cecchetti, Serena; Ragno, Rino; Angiolella, Letizia

    2014-01-01

    Candidosis is the most important cause of fungal infections in humans. The yeast Candida albicans can form biofilms, and it is known that microbial biofilms play an important role in human diseases and are very difficult to treat. The prolonged treatment with drugs has often resulted in failure and resistance. Due to the emergence of multidrug resistance, alternatives to conventional antimicrobial therapy are needed. This study aims to analyse the effects induced by essential oil of Mentha suaveolens Ehrh (EOMS) on Candida albicans and its potential synergism when used in combination with conventional drugs. Morphological differences between control and EOMS treated yeast cells or biofilms were observed by scanning electron microscopy and transmission electron microscopy (SEM and TEM resp.,). In order to reveal the presence of cell cycle alterations, flow cytometry analysis was carried out as well. The synergic action of EOMS was studied with the checkerboard method, and the cellular damage induced by different treatments was analysed by TEM. The results obtained have demonstrated both the effects of EOMS on C. albicans yeast cells and biofilms and the synergism of EOMS when used in combination with conventional antifungal drugs as fluconazole (FLC) and micafungin (MCFG), and therefore we can hypothesize on its potential use in therapy. Further studies are necessary to know its mechanism of action.

  5. Retention of aroma compounds from Mentha piperita essential oil by cyclodextrins and crosslinked cyclodextrin polymers.

    Science.gov (United States)

    Ciobanu, A; Mallard, I; Landy, D; Brabie, G; Nistor, D; Fourmentin, S

    2013-05-01

    In this paper, the controlled release of aroma compounds from cyclodextrins (CDs) and CD polymers was studied by multiple headspace extraction (MHE) experiments. Mentha piperita essential oil was obtained by Soxhlet extraction and identification of the major compounds was performed by GC-MS analysis. Menthol, menthone, pulegone and eucalyptol were identified as the major components. Retention of standard compounds in the presence of different CDs and CD polymers has been realised by static headspace gas chromatography (SH-GC) at 25 °C in the aqueous or gaseous phase. Stability constants for standard compounds and for compounds in essential oil have been also determined with monomeric CD derivatives. The obtained results indicated the formation of a 1:1 inclusion complex for all the studied compounds. Molecular modelling was used to investigate the complementarities between host and guest. This study showed that β-CDs were the most versatile CDs and that β-CD polymers could perform the controlled release of aroma compounds.

  6. Influence of drying on the flavor quality of spearmint (Mentha spicata L.).

    Science.gov (United States)

    Díaz-Maroto, M Consuelo; Pérez-Coello, M Soledad; González Viñas, M A; Cabezudo, M Dolores

    2003-02-26

    Spearmint (Mentha spicata L.) was dried using three different drying methods: oven-drying at 45 degrees C, air-drying at ambient temperature, and freeze-drying. The effect of the drying method on the volatile compounds and on the structural integrity and sensory characteristics of the spice was evaluated. The volatile components from fresh and dried spearmint samples were isolated by simultaneous distillation-extraction (SDE) and analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 28 compounds were identified, carvone, limonene, and 1,8-cineole, in that order, being the main components in all of the samples. Oven-drying at 45 degrees C and air-drying at ambient temperature were the methods that produced the best results. An increase in monoterpenes was observed in all of the dried samples, except in the freeze-dried samples that underwent freezing at -198 degrees C. Freeze-drying resulted in substantial losses in oxygenated terpenes and sesquiterpenes. The effect of each drying method on leaf structure was observed by scanning electron microscopy. From a sensory standpoint, drying the spearmint brought about a decrease in herbaceous and floral notes together with an increase in minty odor.

  7. Phytochemical Properties of Mentha longifolia L. Essential Oil and its Antimicrobial Effects on Staphylococcus Aureus

    Directory of Open Access Journals (Sweden)

    R Mahmodi

    2011-12-01

    Full Text Available Background & Aim: Due to the side effects of chemical and synthetic preservatives, consumers have recently become more eager to use foods containing natural preservatives from plants, animals and microbial sources. In the present study, biochemical composition and antibacterial effects (MIC of Mentha longifolia L. essential oil against Staphylococcus aureus have been evaluated. Methods: In this experimental study, the biochemical composition and antibacterial prosperities of this essential oil was determined by the Gas chromatography/ mass spectrophotometer (GC/MS and micro dilution method respectively. The morphological and membrane changes of the bacterial cell under the effect of this essential oil were evaluated by transmission electron microscopy. The collected data was analyzed by the SPSS software using ANOVA. Results: The chemical analysis of the essential oil by Gas chromatography/ mass spectrophotometer (GC/MS revealed the presence of 22 substances (95.30%, mainly including Pulegon (31.54%, 1,8 Cineol (15.89%, Menthoforan (11.8% and Cis- Isopulegon (9.74%. Minimum inhibitory concentration of the essential oil determined under different temperature and pH values showed to be in the range of 75-1200 µg/ ml. Conclusion: The MIC results and membrane cell damage observed in the electron microscopy evaluation indicated that this essential oil have a high antibacterial activity. Therefore, this essential oil can be combined with other agents for the preservation of foods against pathogenic and toxigenic microorganisms.

  8. Research Progress of Chemical Compositions and Pharmacological Actions of Mentha canadensis%薄荷化学成分与药理作用研究新进展

    Institute of Scientific and Technical Information of China (English)

    沈梅芳; 李小萌; 单琪媛

    2012-01-01

    Through searching literatures about Mentha canadensis at home and abroad in recent years,it is to summarize the chemical compositions,pharmacological actions and modern application in clinic, providing theory foundation for further developing of Mentha canadensis.%通过对薄荷在近几年国内外化学研究、药理作用方面相关文献的查阅,以及现代临床应用等方面的研究情况进行了总结,为进一步合理开发利用薄荷提供理论依据.

  9. Variabilité de la composition chimique des huiles essentielles de Mentha rotundifolia du Nord de l’Algérie

    OpenAIRE

    Georges Lognay; Annabelle Carlier; Michel Marlier; Moussa Brada; Mohamed Bezzina

    2007-01-01

    Variability of the chemical composition of Mentha rotundifolia from Northern Algeria. Algerian oils of Mentha rotundifolia (L.) Huds., obtained by steam distillation (yield : 0,8%), have been analysed by means of GC-FID and GC/MS. The results indicate clearly the occurrence of two chemotypes which are presently described. The former contains high proportions of piperitone oxide (19,7–31,4%) and piperitenone oxide (27,8–29,4%) respectively and the latter revealed piperitenone (54,9%) and piper...

  10. Variabilité de la composition chimique des huiles essentielles de Mentha rotundifolia du Nord de l’Algérie

    Directory of Open Access Journals (Sweden)

    Georges Lognay

    2007-01-01

    Full Text Available Variability of the chemical composition of Mentha rotundifolia from Northern Algeria. Algerian oils of Mentha rotundifolia (L. Huds., obtained by steam distillation (yield : 0,8%, have been analysed by means of GC-FID and GC/MS. The results indicate clearly the occurrence of two chemotypes which are presently described. The former contains high proportions of piperitone oxide (19,7–31,4% and piperitenone oxide (27,8–29,4% respectively and the latter revealed piperitenone (54,9% and piperitenone oxide (17,6% as the major constituents. Such particular composition is reported for the first time within the M. rotundifolia species.

  11. Essential Oil Composition of Menthol Mint (Mentha arvensis) and Peppermint (Mentha piperita) Cultivars at Different Stages of Plant Growth from Kumaon Region of Western Himalaya

    OpenAIRE

    Verma, R S; Rahman, L.; Verma, R. K.; Chauhan, A.; A K Yadav; A Singh

    2010-01-01

    Menthol mint (Mentha arvensis L.) and peppermint (M. piperita L.) cultivars grown in Kumaon region were evaluated for essential oil content and composition at different stages of crop growth. In men­thol mint cultivars viz., ‘Kosi’, ‘Saksham’, ‘Himalaya’, and ‘Kalka’, the essential oil content was found to vary from 0.3% - 1.2%, 0.42% - 1.1%, 0.38% - 1.0% and 0.26% - 1.2%, at different days after transplanting (DAT) respectively, while in cultivars ‘Kukrail’, ‘CIM-Madhurus’ and ‘CIM-Indus’ of...

  12. 薄荷总黄酮提取工艺研究%Extraction Technology for Total Flavonoids from Mentha Haplocalyx

    Institute of Scientific and Technical Information of China (English)

    张秋燕; 徐晓岚; 张福平

    2011-01-01

    Objective: To study the optimum methanol extraction process of total flavonoids in Mentha haplocalyx. Methods: With Rutin as a standard and the Mentha haplocalyx as the subject, the paper studied the extraction of the flavonoids from Mentha haplocalyx with orthogonal test. It was extracted by ethanol solvent.And the percent was tested by spectrophotometer. Results: The factors which influenced the extraction rate were sorted as follows: concentration of ethanol > reaction time > ratio of liquid to solid >reaction temperature. Conclusion: The optimum extracting conditions were: ethanol concentration 95%, the ratio of liquid to solid as 15: 1, reaction time 1h, reaction temperature 80℃ .%目的:优选薄荷中总黄酮的提取工艺.方法:以薄荷为原料,以芦丁为标准品,以乙醉为溶剂,通过正交实验对薄荷黄酮的提取进行研究,并用分光光度法测定黄酮类物质的含量.结果:影响薄荷总黄酮提取效果的主次因素为:乙醉浓度>提取时间>固液比>提取温度.结论:黄酮最佳提取工艺为乙醉浓度95%,固液比1:15,提取时间lh,提取温度80℃.

  13. Mentha spicata Essential Oil: Chemical Composition, Antioxidant and Antibacterial Activities against Planktonic and Biofilm Cultures of Vibrio spp. Strains

    OpenAIRE

    Mejdi Snoussi; Emira Noumi; Najla Trabelsi; Guido Flamini; Adele Papetti; Vincenzo De Feo

    2015-01-01

    Chemical composition, antioxidant and anti-Vibrio spp. activities of the essential oil isolated from the aerial parts of Mentha spicata L. (spearmint) are investigated in the present study. The effect of the essential oil on Vibrio spp. biofilm inhibition and eradication was tested using the XTT assay. A total of 63 chemical constituents were identified in spearmint oil using GC/MS, constituting 99.9% of the total identified compounds. The main components were carvone (40.8% ± 1.23%) and lim...

  14. In-vivo Antioxidant Effects of Ethyl Acetate Fraction of Mentha spicata L. on 4-Nitroquinoline-1-Oxide Injected Mice

    OpenAIRE

    Arumugam, Ponnan; Ramesh, Arabandi

    2011-01-01

    Antioxidant effects of ethyl acetate fraction of Mentha spicata (L.) were evaluated against 4-nitroquinoline-1-oxide injected mice. For this study, experiment setup consisted of 36 albino mice of either sex divided into 6 groups: Control (25% DMSO in water), ethyl acetate fraction (EAF) alone group (80, 160 mg/Kg body weight-bwt), 4-NQO (7.5 mg/Kg bwt-IP) alone and 4-NQO + EAF. EAF and vehicles were administered orally for five consecutive days. 4-NQO (7.5 mg/Kg bwt) was injected intraperiton...

  15. Comparison between the essential oil and supercritical Carbon Dioxide extraction of Mentha Piperita L. cultivated in Iran

    OpenAIRE

    Aghel N; Yamini Y; Hadji Akhoondi A

    2002-01-01

    The chemical compositions of the essential oils and the volatile concentrate of Mentha piperita L. (Labiatae) obtained by supercritical carbon dioxide extraction (SFE) at 35°C and 100 atm were compared using GC/MS. Whereas twenty four components were identified in the essential oil, only seven compounds, including the main compounds of the peppermint oil were isolated by the SFE. The percent of major components of the oil and the extract were: Menthol (31.53 and 48.39), Menthone (23.37 and 26...

  16. Variation in light absorption properties of mentha aquatica L. as a function of leaf form: Implications for plant growth

    DEFF Research Database (Denmark)

    Enriquez, Susana; Jensen, Kaj Sand

    2008-01-01

    area increased. This relationship indicates that dispersive samples, such as leaves, although efficient light traps, can also be affected by the "package effect." Mentha aquatica leaves, by expanding their biomass (increased specific leaf area [SLA]), improve their light absorption efficiency per unit...... photon may result in a reduction in the specific plant growth rate (RGR). Understanding the association between leaf form and the ability of leaf biomass to absorb light provides a mechanistic explanation for the empirical relationship found repeatedly in the literature between RGR and SLA. Our results...

  17. IMPACT OF ANISE (PIMPINELLA ANISUM) AND MINT (MENTHA PIPERITA) ESSENTIAL OILS TO MICROBIAL ACTIVITY IN CHICKEN MEAT

    OpenAIRE

    Miroslava Kačániová; Jana Petrová; Martin Mellen; Juraj Čuboň; Peter Haščík; Lukáš Hleba; Margarita Terentjeva; Simona Kunová; Henrieta Blaškovičová

    2015-01-01

    The goal of the present study was to evaluate fresh chicken thighs quality (microbiological and sensory) after treatment by ethylenediaminetetraacetate (EDTA), Pimpinella anisum L. and Mentha piperita essential oils in 1% concentration, stored under vacuum packaging (VP), at 4±0.5°C for a period of 16 days. The following treatments of chicken thighs were applied: air-packaged (AC, control samples), vacuum-packaged (VPC, control samples), vacuum-packed with EDTA solution 1.50% v/w (VPEC, contr...

  18. UV-B modulates the interplay between terpenoids and flavonoids in peppermint (Mentha x piperita L.).

    Science.gov (United States)

    Dolzhenko, Yuliya; Bertea, Cinzia M; Occhipinti, Andrea; Bossi, Simone; Maffei, Massimo E

    2010-08-01

    Modulation of secondary metabolites by UV-B involves changes in gene expression, enzyme activity and accumulation of defence metabolites. After exposing peppermint (Mentha x piperita L.) plants grown in field (FP) and in a growth chamber (GCP) to UV-B irradiation, we analysed by qRT-PCR the expression of genes involved in terpenoid biosynthesis and encoding: 1-deoxy-D-xylulose-5-phosphate synthase (Dxs), 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase (Mds), isopentenyl diphosphate isomerase (Ippi), geranyl diphosphate synthase (Gpps), (-)-limonene synthase (Ls), (-)-limonene-3-hydroxylase (L3oh), (+)-pulegone reductase (Pr), (-)-menthone reductase (Mr), (+)-menthofuran synthase (Mfs), farnesyl diphosphate synthase (Fpps) and a putative sesquiterpene synthase (S-TPS). GCP always showed a higher terpenoid content with respect to FP. We found that in both FP and GCP, most of these genes were regulated by the UV-B treatment. The amount of most of the essential oil components, which were analysed by gas chromatography-mass spectrometry (GC-MS), was not correlated to gene expression. The total phenol composition was found to be always increased after UV-B irradiation; however, FP always showed a higher phenol content with respect to GCP. Liquid chromatography-mass spectrometry (LC-ESI-MS/MS) analyses revealed the presence of UV-B absorbing flavonoids such as eriocitrin, hesperidin, and kaempferol 7-O-rutinoside whose content significantly increased in UV-B irradiated FP, when compared to GCP. The results of this work show that UV-B irradiation differentially modulates the expression of genes involved in peppermint essential oil biogenesis and the content of UV-B absorbing flavonoids. Plants grown in field were better adapted to increasing UV-B irradiation than plants cultivated in growth chambers. The interplay between terpenoid and phenylpropanoid metabolism is also discussed.

  19. [Effect of drying methods on monoterpenes, phenolic acids and flavonoids in Mentha haplocalyx].

    Science.gov (United States)

    Zhu, Shao-qing; Zhu, Zhen-hua; Guo, Sheng; Zhao, Yu-yang; Lu, Xue-jun; Sha, Xiu-xiu; Qian, Da-wei; Duan, Jin-ao

    2015-12-01

    To provide a scientific basis for the selection of the appropriate drying method for Mentha Haplocalyx Herba (MHH), determine 2 monoterpenes, 4 phenolic acids and 5 flavonoids in MHH by GC-MS and UPLC-TQ-MS methods, and investigate the effects of the drying methods on the changes in contents of these analytes. The qualities of products obtained with different drying methods were evaluated by the multivariate statistical method of Technique for Order Preference by Similarity to Ideal Solution (TOPSIS). Results showed that the drying methods had the greatest impact on menthol, caffeic acid, and rosemary acid, which were followed by chlorogenic acid and diosmetin-7-O-glucoside. The contents in these analytes processed with hot-air-drying method were higher than those with microwave-drying and infrared-drying methods at the same temperatures. The contents in these analytes processed under low temperature (40-45 °C) were higher than those under higher temperature (60-70 °C). Above all, the contents in phenolic acids processed with microwave fixation (exposed under microwave at 100 °C for several minutes) were obviously higher than those of not being processed, showing an inhibition of some enzymes in samples after fixation. The TOPSIS evaluation showed that the variable temperature drying method of 'Hot-Air 45-60 °C' was the most suitable approach for the primary drying processing of MHH. The results could provide the scientific basis for the selection of appropriate drying method for MHH, and helpful reference for the primary drying proces of herbs containing volatile chemical components. PMID:27245035

  20. Bioefficacy of Mentha piperita essential oil against dengue fever mosquito Aedes aegypti L

    Science.gov (United States)

    Kumar, Sarita; Wahab, Naim; Warikoo, Radhika

    2011-01-01

    Objective To assess the larvicidal and repellent potential of the essential oil extracted from the leaves of peppermint plant, Mentha piperita (M. piperita) against the larval and adult stages of Aedes aegypti (Ae. Aegypti). Methods The larvicidal potential of peppermint oil was evaluated against early fourth instar larvae of Ae. aegypti using WHO protocol. The mortality counts were made after 24 and 48 h, and LC50 and LC90 values were calculated. The efficacy of peppermint oil as mosquito repellent was assessed using the human-bait technique. The measured area of one arm of a human volunteer was applied with the oil and the other arm was applied with ethanol. The mosquito bites on both the arms were recorded for 3 min after every 15 min. The experiment continued for 3 h and the percent protection was calculated. Results The essential oil extracted from M. piperita possessed excellent larvicidal efficiency against dengue vector. The bioassays showed an LC50 and LC90 value of 111.9 and 295.18 ppm, respectively after 24 h of exposure. The toxicity of the oil increased 11.8% when the larvae were exposed to the oil for 48 h. The remarkable repellent properties of M. piperita essential oil were established against adults Ae. aegypti. The application of oil resulted in 100% protection till 150 min. After next 30 min, only 1-2 bites were recorded as compared with 8-9 bites on the control arm. Conclusions The peppermint essential oil is proved to be efficient larvicide and repellent against dengue vector. Further studies are needed to identify the possible role of oil as adulticide, oviposition deterrent and ovicidal agent. The isolation of active ingredient from the oil could help in formulating strategies for mosquito control. PMID:23569733

  1. [Effect of drying methods on monoterpenes, phenolic acids and flavonoids in Mentha haplocalyx].

    Science.gov (United States)

    Zhu, Shao-qing; Zhu, Zhen-hua; Guo, Sheng; Zhao, Yu-yang; Lu, Xue-jun; Sha, Xiu-xiu; Qian, Da-wei; Duan, Jin-ao

    2015-12-01

    To provide a scientific basis for the selection of the appropriate drying method for Mentha Haplocalyx Herba (MHH), determine 2 monoterpenes, 4 phenolic acids and 5 flavonoids in MHH by GC-MS and UPLC-TQ-MS methods, and investigate the effects of the drying methods on the changes in contents of these analytes. The qualities of products obtained with different drying methods were evaluated by the multivariate statistical method of Technique for Order Preference by Similarity to Ideal Solution (TOPSIS). Results showed that the drying methods had the greatest impact on menthol, caffeic acid, and rosemary acid, which were followed by chlorogenic acid and diosmetin-7-O-glucoside. The contents in these analytes processed with hot-air-drying method were higher than those with microwave-drying and infrared-drying methods at the same temperatures. The contents in these analytes processed under low temperature (40-45 °C) were higher than those under higher temperature (60-70 °C). Above all, the contents in phenolic acids processed with microwave fixation (exposed under microwave at 100 °C for several minutes) were obviously higher than those of not being processed, showing an inhibition of some enzymes in samples after fixation. The TOPSIS evaluation showed that the variable temperature drying method of 'Hot-Air 45-60 °C' was the most suitable approach for the primary drying processing of MHH. The results could provide the scientific basis for the selection of appropriate drying method for MHH, and helpful reference for the primary drying proces of herbs containing volatile chemical components.

  2. Cytotoxic activity of methanolic extract of Mentha longifolia and Ocimum basilicum against human breast cancer.

    Science.gov (United States)

    Al-Ali, Khalil H; El-Beshbishy, Hesham A; El-Badry, Ayman A; Alkhalaf, Moussa

    2013-12-01

    Labiatae family is represented in Saudi Arabia. The aim of the present study was to go insight to investigate the anticancer activity and antioxidative potentials of methanolic extracts of Mentha longifolia L. (ML) and Ocimum basilicum L. (OB) that grown in Madina province, western region, Saudi Arabia. OB exhibited the greater phenolic contents as mg gallic acid equivalent/g weight (mg GAE/g) for a value of 105 +/- 5.5 mg GAE/g. On the other hand, ML produced 29 +/- 3.12 mg GAE/g. The standard antioxidant vitamin E used in this experiment elicited a value of total phenolic contents equal 22 +/- 2.2 mg GAE/g. The percentage scavenging activity of against diphenylpicrylhydrazyl (DPPH) was 850 and 160% for OB and ML extracts, respectively. Vitamin E elicited% scavenging activity of against DPPH equal to 198%. Brine shrimp cytotoxic assay clearly indicated the cytotoxic effects of either ML or OB extract. The brine shrimp survival is inversely proportional to the concentration of either ML or OB extract used with LD50 191.23 and 235.50 ppm, respectively. Toxic effects on brine shrimps indicated the anticancer potential of ML or OB extract. The ML or OB extract was unable to produce pbluescript (pBS) plasmid DNA damage, while the plasmid DNA treated with EcoRI produced a single band as a result of DNA damage. Also, both ML and OB extract exhibited marked cytotoxic activity against MCF-7 cells at various concentrations (20, 40, 80, 160 and 320 microg mL(-1)). The 160 and 320 microg mL(-1) showed more cytotoxic effect against MCF-7 cells. Based on results achieved, we can concluded that, OB and ML extracts have the potency to act as powerful antioxidants and protect against DNA damage and have cytotoxic activity against MCF-7 cell line.

  3. Antibacterial activity of leaves and inter-nodal callus extracts of Mentha arvensis L

    Institute of Scientific and Technical Information of China (English)

    JohnsonM; WeselyEG; KavithaMS; UmaV

    2011-01-01

    Objective:To determine the anti-bacterial efficacy of chloroform, ethanol, ethyl acetate and water extracts of inter-nodal and leaves derived calli extracts from Mentha arvensis (M. arvensis) against Salmonella typhi(S. typhi), Streptococcus pyogenes(S. pyogenes), Proteus vulgaris(P. vulgaris) and Bacillus subtilis(B. subtilis). Methods: The inter-nodal and leaves segments of M. arvensis were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3% sucrose, gelled with 0.7% agar and different concentration of 2, 4-Dichlorophenoxyacetie acid (2,4-D) either alone or in combinations. The preliminary phytochemical screening was performed by Brindha et al method. Antibacterial efficacy was performed by disc diffusion method and incubated for 24 h at 37 ℃. Results: Maximum percentage of callus formation (inter-nodal segments 84.3±0.78;leaves segments 93.8±1.27) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 1.5 mg/L of 2, 4-D. The ethanol extracts of leaves derived calli showed the maximum bio-efficacy than other solvents. The leaves and stem derived calli extracts on Proteus sp. showed that the plants can be used in the treatment of urinary tract infection associated with Proteus sp. Through the bacterial efficacy studies, it is confirmed that the in vitro raised calli tissue was more effective compared to in vivo tissue. Conclusions:The bio-efficacy study confirmed that the calli mediated tissues showed the maximum zone of inhibition. The present study paved a protocol to establish high potential cell lines by in vitro culture.

  4. Some aspects of salinity responses in peppermint (Mentha × piperita L.) to NaCl treatment.

    Science.gov (United States)

    Li, Zhe; Yang, Hetong; Wu, Xiaoqing; Guo, Kai; Li, Jishun

    2015-05-01

    Salinity is a major stress that adversely affects plant growth and crop production. Understanding the cellular responses and molecular mechanisms by which plants perceive and adopt salinity stress is of fundamental importance. In this work, some of the cellular signaling events including cell death, reactive oxygen species (ROS) generation, and the behaviors of organelles were analyzed in a salt-tolerant species (Keyuan-1) of peppermint (Mentha × piperita L.) under NaCl treatment. Our results showed that 200 mM NaCl treatment elicited a distinct progress of cell death with chromatin condensation and caspase-3-like activation and a dramatic burst of ROS which was required for the execution of cell death. The major ROS accumulation occurred in the mitochondria and chloroplasts, which were the sources of ROS production under NaCl stress. Moreover, mitochondrial activity and photosynthetic capacity also exhibited the obvious decrease in the ROS-dependent manner under 200 mM NaCl stress. Furthermore, the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), and dehydroascorbate reductase (DHAR) as well as the contents of ascorbate and glutathione changed in the concentration-dependent manner under NaCl stress. Altogether, our data showed the execution of programmed cell death (PCD), the ROS dynamics, and the behaviors of organelles especially mitochondria and chloroplasts in the cellular responses of peppermint to NaCl stress which can be used for the tolerance screening, and contributed to the understanding of the cellular responses and molecular mechanisms of peppermint to salinity stress, providing the theoretic basis for the further development and utilization of peppermint in saline areas.

  5. Effect of Cadmium and Lead on Quantitative and Essential Oil Traits of Peppermint (Mentha piperita L.

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    Shahram AMIRMORADI

    2012-11-01

    Full Text Available Cadmium (Cd and lead (Pb are particularly noteworthy metals that can pollute the air, soil and water contributing to serious environmental problems. Tests were done on concentrations of Pb and Cd; treatments tested in the experiment were as follows; Cd concentrations (10, 20, 40, 60, 80, 100 ppm and concentrations of Pb (100, 300, 600, 900, 1200, 1500 ppm and control. Tests were done on Mentha piperita L. in a greenhouse, arranged as a randomized complete block design with three replications. Rhizomes with uniform weight were planted in pots 30�50�35 cm. Plants were irrigated with Cd and Pb chloride after germination of all rhizomes. Results demonstrated that with increasing concentrations of Cd and Pb there was a decrease in fresh and dry weights, main stem height, leaf area per plant, leaf number, number of nodes per main stem and essential oil of peppermint compared to the control. Fresh weights were decreased at 100 ppm of Cd and 1500 ppm of Pb, 18.16% and 24.55%, respectively compared to the control at the first harvest. At the second harvest, these decreases were 15.24% and 32.72%, respectively. At the highest concentrations of Cd and Pb, dry weight of peppermint was dropped 22.92% and 39.01% at the first harvest. For the second harvest, decreased dry weights were 25.88% and 26.77% respectively. It seems that peppermint can tolerate waste water or soil polluted with medium range of Cd and Pb concentrations and the essential oil percentage was not affected by these concentrations.

  6. Encapsulation of Mentha Oil in Chitosan Polymer Matrix Alleviates Skin Irritation.

    Science.gov (United States)

    Mishra, Nidhi; Rai, Vineet Kumar; Yadav, Kuldeep Singh; Sinha, Priyam; Kanaujia, Archana; Chanda, Debabrata; Jakhmola, Apurva; Saikia, Dharmendra; Yadav, Narayan Prasad

    2016-04-01

    Mentha spicata L. var. viridis oil (MVO) is a potent antifungal agent, but its application in the topical treatment is limited due to its irritancy and volatility. It was aimed to develop more efficient, chitosan-incrusted MVO microspheres with reduced volatility and lesser irritancy and to dispense it in the form of ointment. Simple coacervation technique was employed to microencapsulate MVO in chitosan matrix. Morphological properties and polymer cross-linking were characterized by scanning electron microscopy and differential scanning calorimetry, respectively. Optimization was carried out on the basis of entrapment efficiency (EE) using response surface methodology. Well-designed microspheres having smooth surface and spherical shape were observed. EE (81.20%) of optimum batch (R21) was found at 1.62% w/v of cross-linker, 5.4:5 of MVO to chitosan ratio and at 1000 rpm. R21 showed 69.38 ± 1.29% in vitro MVO release in 12 h and 96.92% retention of MVO in microspheres even after 8 week. Ointments of PEG 4000 and PEG 400 comprising MVO (F1) and R21 (F2) were developed separately. F2 showed comparatively broader zone of growth inhibition (13.33 ± 1.76-18.67 ± 0.88 mm) and less irritancy (PII 0.5833, irritation barely perceptible) than that of F1. F2 was able to avoid the direct contact of mild irritant MVO with the skin and to reduce its rapid volatility. Controlled release of MVO helped in lengthening the duration of availability of MVO in agar media and hence improved its therapeutic efficacy. In conclusion, a stable and non-irritant formulation with improved therapeutic potential was developed. PMID:26248538

  7. Chemical Composition and Antibacterial Activity of the Essence oil of Peppermint (Mentha piperita L

    Directory of Open Access Journals (Sweden)

    Z Izadi

    2009-10-01

    Full Text Available Introduction & Objective: Peppermint (Mentha piperita L. is a perennial herbaceous essence oil bearing plant which belongs to the Lamiaceae family. This plant is a valuable and important herb which has many therapeutic properties. Recent investigations have shown its excellent anti-irritable bowel syndrome effects. Other properties of this plant are anti-inflammatory, analgesic, promote menstrual flow, antipyretic, antiseptic and anti-rheumatoid effects. This investigation was conducted to study the antibacterial properties of peppermint essence oil, as well as determining the content and composition of essential oil. Materials & Methods: In order to study the effect of the antibacterial activity of the essence oil of peppermint, this experimental study was conducted in 2009 at Hamedan University of Medical Sciences. The aerial parts of the peppermint were harvested in summer when it was in the full blooming stage of the plant. The collected aerial parts were then dried in the shade. The essence oil of the aerial parts was extracted by hydro-distillation technique using Clevenger apparatus and was analyzed by the capillary GC and GC/MS method. Anti bacterial properties of the essence oil on four pathogenic bacteria were determined by using broth dilution and well diffusion agar methods. The collected data were analyzed by the SPSS version 11.5 software, using the independent t-test. Results: The essence oil of peppermint showed the maximum anti bacterial effect on E. coli and the minimum effect on S. aureus and P. aeuroginosa. The essence oil content of aerial parts was 0.69% (w/w based on dry weight. The amount of menthol which is the main constituent of the oil and as an antiseptic component was 47.9%. Conclusion: Results of this study revealed that the essential oil of peppermint is rich in menthol and can be considered as an anti-bacterial agent in drug and food industries.

  8. PEG-induced osmotic stress in Mentha x piperita L.: Structural features and metabolic responses.

    Science.gov (United States)

    Búfalo, Jennifer; Rodrigues, Tatiane Maria; de Almeida, Luiz Fernando Rolim; Tozin, Luiz Ricardo Dos Santos; Marques, Marcia Ortiz Mayo; Boaro, Carmen Silvia Fernandes

    2016-08-01

    The present study investigated whether osmotic stress induced by the exposure of peppermint (Mentha x piperita L.) to moderate and severe stress for short periods of time changes the plant's physiological parameters, leaf anatomy and ultrastructure and essential oil. Plants were exposed to two levels of polyethyleneglycol (50 g L(-1) and 100 g L(-1) of PEG) in a hydroponic experiment. The plants exposed to 50 g L(-1) maintained metabolic functions similar to those of the control group (0 g L(-1)) without changes in gas exchange or structural characteristics. The increase in antioxidant enzyme activity reduced the presence of free radicals and protected membranes, including chloroplasts and mitochondria. In contrast, the osmotic stress caused by 100 g L(-1) of PEG inhibited leaf gas exchange, reduced the essential oil content and changed the oil composition, including a decrease in menthone and an increase in menthofuran. These plants also showed an increase in peroxidase activity, but this increase was not sufficient to decrease the lipid peroxidation level responsible for damaging the membranes of organelles. Morphological changes were correlated with the evaluated physiological features: plants exposed to 100 g L(-1) of PEG showed areas with collapsed cells, increases in mesophyll thickness and the area of the intercellular space, cuticle shrinkage, morphological changes in plastids, and lysis of mitochondria. In summary, our results revealed that PEG-induced osmotic stress in M. x piperita depends on the intensity level of the osmotic stress applied; severe osmotic stress changed the structural characteristics, caused damage at the cellular level, and reduced the essential oil content and quality.

  9. Isolation and identification of antimicrobial compound from Mentha longifolia L. leaves grown wild in Iraq

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    Al-Bayati Firas A

    2009-06-01

    Full Text Available Abstract Background Mentha longifolia L. (Lamiaceae leaves have been traditionally implemented in the treatment of minor sore throat and minor mouth or throat irritation by the indigenous people of Iraq, although the compounds responsible for the medicinal properties have not been identified. In the present study, an antimicrobial compound was isolated and characterized, and its biological activity was assessed. Methods The compound was isolated and characterized from the extracted essential oil using different spectral techniques: TLC, FTIR spectra and HPLC. Antimicrobial activity of the compound was assessed using both disc diffusion and microdilution method in 96 multi-well microtiter plates. Results A known compound was isolated from the essential oil of the plant and was identified as (- menthol. The isolated compound was investigated for its antimicrobial activity against seven selected pathogenic and non-pathogenic microorganisms: Staphylococcus aureus, Streptococcus mutans, Streptococcus faecalis, Streptococcus pyogenis, Lactobacillus acidophilus, Pseudomonas aeruginosa and the yeast Candida albicans. Menthol at different concentrations (1:1, 1:5, 1:10, 1:20 was active against all tested bacteria except for P. aeruginosa, and the highest inhibitory effect was observed against S. mutans (zone of inhibition: 25.3 mm using the disc diffusion method. Minimal inhibitory concentration MIC values ranged from 15.6–125.0 μg/ml, and the most promising results were observed against S. aureus and S. mutans (MIC 15.6 μg/ml while, S. faecalis, S. pyogenis and L. acidophilus ranked next (MIC 31.2 μg/ml. Furthermore, menthol achieved considerable antifungal activity against the yeast C. albicans (zone of inhibition range: 7.1–18.5 mm; MIC: 125.0. Conclusion The isolation of an antimicrobial compound from M. longifolia leaves validates the use of this plant in the treatment of minor sore throat and minor mouth or throat irritation.

  10. Encapsulation of Mentha Oil in Chitosan Polymer Matrix Alleviates Skin Irritation.

    Science.gov (United States)

    Mishra, Nidhi; Rai, Vineet Kumar; Yadav, Kuldeep Singh; Sinha, Priyam; Kanaujia, Archana; Chanda, Debabrata; Jakhmola, Apurva; Saikia, Dharmendra; Yadav, Narayan Prasad

    2016-04-01

    Mentha spicata L. var. viridis oil (MVO) is a potent antifungal agent, but its application in the topical treatment is limited due to its irritancy and volatility. It was aimed to develop more efficient, chitosan-incrusted MVO microspheres with reduced volatility and lesser irritancy and to dispense it in the form of ointment. Simple coacervation technique was employed to microencapsulate MVO in chitosan matrix. Morphological properties and polymer cross-linking were characterized by scanning electron microscopy and differential scanning calorimetry, respectively. Optimization was carried out on the basis of entrapment efficiency (EE) using response surface methodology. Well-designed microspheres having smooth surface and spherical shape were observed. EE (81.20%) of optimum batch (R21) was found at 1.62% w/v of cross-linker, 5.4:5 of MVO to chitosan ratio and at 1000 rpm. R21 showed 69.38 ± 1.29% in vitro MVO release in 12 h and 96.92% retention of MVO in microspheres even after 8 week. Ointments of PEG 4000 and PEG 400 comprising MVO (F1) and R21 (F2) were developed separately. F2 showed comparatively broader zone of growth inhibition (13.33 ± 1.76-18.67 ± 0.88 mm) and less irritancy (PII 0.5833, irritation barely perceptible) than that of F1. F2 was able to avoid the direct contact of mild irritant MVO with the skin and to reduce its rapid volatility. Controlled release of MVO helped in lengthening the duration of availability of MVO in agar media and hence improved its therapeutic efficacy. In conclusion, a stable and non-irritant formulation with improved therapeutic potential was developed.

  11. Acute toxicity and metabolomics analysis of hypocholesterolemic effect of Mentha piperita aqueous extract in Wistar rats

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    Nor Zaini Johari

    2015-01-01

    Full Text Available Summary. The oral acute toxicity of the aqueous peppermint extract (APE was assessed and the bio and/or chemo markers for hypocholesterolemic activity of APE were identified through metabolomics approach. No mortality resulted from the present oral acute toxicity study in which the histological changes observed in the selected organs and the biochemical deviation of blood compared to the normal range level were minimal. This study also explored the effect of 290 mg-1 kg body weight of APE against 5% cholesterol-enriched diet within 14 days treatment. Whereby after the treatment, there were reductions exhibited in plasma total cholesterol (44.32%, LDL-cholesterol (69.19% and total triglycerides (55.77%. 1H NMR-metabolomics approach was, employed for better sensitivity and accuracy in evaluating the  potential plasma biomarkers of hyper-and hypo-cholesterolemic properties. β-Hydroxybutarate and α-D-glucose have been identified as the possible hypercholesterolemic markers, whereas taurine, betaine, alanine, glycine and L-leucine were suggested to be the hypocholesterolemic markers of APE.  Industrial relevance. Due to various reports on the side effects of conventional drug-lowering cholesterol available in the market, aqueous peppermint extract at its recommended consumption dosage has been investigated over its toxicity of oral consumption and its efficacy against elevation of cholesterol level in blood. The evaluation of hypocholesterolemic activity of aqueous peppermint extract (APE, from which the potential biomarkers could be established, might be useful in the  development of new anti-cholesterol drug and also for quality control of peppermint-based products. Keywords. Mentha piperita; peppermint; hypercholesterolemia; metabolomics; 1H NMR; multivariate data analysis

  12. Investigation of Antibacterial Activity of Ethanolic and Methanolic Extracts of Mentha pulegium L.

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    Hossein Motamedi

    2014-10-01

    Full Text Available Background: With regard to the rapid emerging antibiotic resistance bacteria, plants as one of the most common natural sources of antimicrobial agents can be used as alternative for treatment of infectious diseases. This study was designed to investigate antibacterial activity of Mentha pulegium L. (Lamiaceae family. Materials and Methods: In this experimental study, the antibacterial effect of 4, 8, 16 and 24 mg/disc of alcoholic extracts were assessed using standard disc diffusion method. Minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC were also determined. Furthermore, the structural changes following to the exposure with these extracts were also investigated in test bacteria. Results: Both extracts of this plant showed considerable antibacterial activity against some Gram positive (Bacillus cereus and Staphylococcus aureus and also Gram negative (Escherichia coli, Pseudomonas aeruginosa and Proteus mirabilis bacteria. All of the tested bacteria were resistant to nafcillin. The maximum effects was observed in the case of both ethanolic and methanolic extracts in all concentrations on P. mirabilis (25 mm and the lowest effect was on P. aeruginosa. MIC and MBC values of both extracts against S. aureus were equal (MIC=MBC=8 mg/mL and P. mirabilis were MIC=4 mg/mL and MBC=8 mg/mL. The SEM analysis revealed deformation and cell wall disruption of affected bacteria. Conclusion: Based on these results it can be suggested that M. pulegium L. is an effective antibacterial plant that can be used as a new source for antibiotic discovery against bacterial pathogens especially food poisoning pathogens such as S. aureus, B. cereus and also for treatment of P. mirabilis infection.

  13. PEG-induced osmotic stress in Mentha x piperita L.: Structural features and metabolic responses.

    Science.gov (United States)

    Búfalo, Jennifer; Rodrigues, Tatiane Maria; de Almeida, Luiz Fernando Rolim; Tozin, Luiz Ricardo Dos Santos; Marques, Marcia Ortiz Mayo; Boaro, Carmen Silvia Fernandes

    2016-08-01

    The present study investigated whether osmotic stress induced by the exposure of peppermint (Mentha x piperita L.) to moderate and severe stress for short periods of time changes the plant's physiological parameters, leaf anatomy and ultrastructure and essential oil. Plants were exposed to two levels of polyethyleneglycol (50 g L(-1) and 100 g L(-1) of PEG) in a hydroponic experiment. The plants exposed to 50 g L(-1) maintained metabolic functions similar to those of the control group (0 g L(-1)) without changes in gas exchange or structural characteristics. The increase in antioxidant enzyme activity reduced the presence of free radicals and protected membranes, including chloroplasts and mitochondria. In contrast, the osmotic stress caused by 100 g L(-1) of PEG inhibited leaf gas exchange, reduced the essential oil content and changed the oil composition, including a decrease in menthone and an increase in menthofuran. These plants also showed an increase in peroxidase activity, but this increase was not sufficient to decrease the lipid peroxidation level responsible for damaging the membranes of organelles. Morphological changes were correlated with the evaluated physiological features: plants exposed to 100 g L(-1) of PEG showed areas with collapsed cells, increases in mesophyll thickness and the area of the intercellular space, cuticle shrinkage, morphological changes in plastids, and lysis of mitochondria. In summary, our results revealed that PEG-induced osmotic stress in M. x piperita depends on the intensity level of the osmotic stress applied; severe osmotic stress changed the structural characteristics, caused damage at the cellular level, and reduced the essential oil content and quality. PMID:27107175

  14. Bioefficacy ofMentha piperita essential oil against dengue fever mosquito Aedes aegypti L

    Institute of Scientific and Technical Information of China (English)

    Sarita Kumar; Naim Wahab; Radhika Warikoo

    2011-01-01

    Objective:To assess the larvicidal and repellent potential of the essential oil extracted from the leaves of peppermint plant,Mentha piperita (M. piperita) against the larval and adult stages of Aedes aegypti (Ae. Aegypti).Methods: The larvicidal potential of peppermint oil was evaluated against early fourth instar larvae ofAe. aegypti usingWHO protocol. The mortality counts were made after 24 and48 h, and LC50 and LC90values were calculated. The efficacy of peppermint oil as mosquito repellent was assessed using the human-bait technique. The measured area of one arm of a human volunteer was applied with the oil and the other arm was applied with ethanol. The mosquito bites on both the arms were recorded for3 min after every15 min. The experiment continued for 3 h and the percent protection was calculated.Results:The essential oil extracted fromM. piperita possessed excellent larvicidal efficiency against dengue vector. The bioassays showed an LC50 and LC90 value of111.9 and295.18 ppm, respectively after24 h of exposure. The toxicity of the oil increased11.8% when the larvae were exposed to the oil for48 h. The remarkable repellent properties ofM. piperita essential oil were established against adults Ae. aegypti. The application of oil resulted in100% protection till150 min. After next30min, only1-2 bites were recorded as compared with8-9 bites on the control arm.Conclusions:The peppermint essential oil is proved to be efficient larvicide and repellent against dengue vector. Further studies are needed to identify the possible role of oil as adulticide, oviposition deterrent and ovicidal agent. The isolation of active ingredient from the oil could help in formulating strategies for mosquito control.

  15. 灰薄荷精油化学成分研究%Research on Chemical Components of Essential Oil from Mentha vagans Boriss.

    Institute of Scientific and Technical Information of China (English)

    刘艳; 梁呈元; 李维林

    2011-01-01

    目的 研究灰薄荷(Mentha vagans Boriss.)精油的化学成分.方法 通过水蒸汽蒸馏法提取灰薄荷精油,并利用GC-MS对精油化学成分进行了分析.结果 从灰薄荷精油中分离鉴定出20种化学成分,占总精油的97.1%.其中含量较高的为辣薄荷烯酮氧化物(Piperitenone oxide,68.5%)、桉树脑(1,8-Cineole,13.5%)和月桂烯(β-Myrcene,2.1%).结论 分离鉴定了灰薄荷精油的主要化学成分.%Objective To study the chemical components of essential oil from Mentha vagans Boriss.. Methods The essential oil was extracted from Mentha vagans Boriss. By steam distillation, and the chemical components of essential oil were analyzed by GC-MS. Results 20 components were identified which composed of 97.1% of the total essential oil including piperitenone oxide (68.5%), 1,8-cineole (13.5%) and P-myrcene (2.1%). Conclusion The main chemical constituents of Mentha vagans Boriss. Were identified.

  16. Nane (Mentha Spicata L.) ve Kekik (Thymus Vulgaris L.) Bitkileri Kullanarak Yün Lifinin Doğal Boyanması

    OpenAIRE

    TUTAK, Mustafa; ACAR, Gülcan; AKMAN, Onur

    2014-01-01

    In this study, 100% wool fabrics were naturally dyed using mint (Mentha spicata L.) and thyme (Thymus vulgaris L.). Before the dyeing process, fabrics were mordanted with iron (II) sulfate with different concentrations according to pre-mordanting technique. After the natural dyeing process, K/S color yields, CIE L* a * b * color coordinates and various fastness properties of dyed fabrics were determined.

  17. Physiological indexese macro- and micronutrients in plant tissue and essential oil of Mentha piperita L. grown in nutrient solution with variation in N, P, K and Mg levels

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    E.F.S. David

    2014-03-01

    Full Text Available Mentha piperita L. is an aromatic and medicinal species of the family Lamiaceae, known as mint or peppermint, and its leaves and branches produce essential oil rich in menthol. This study aimed to evaluate physiological indexes, macro- and micronutrients inthe shootsand essential oil of Mentha piperita L. grown in nutrient solution number 2 of Hoagland and Arnon (1950 with different N, P, K and Mg levels. Shoot length, dry mass of the different organs, total dry mass, leaf area, essential oil yield and composition, and macronutrient (N, P, K, Mg, Ca, S and micronutrient (Mn, Cu, Fe, Zn contents in the shoot were evaluated. Plants treated with 65%N/50%P/25%K/100%Mg had a tendency towards longer shoot, greaterroot and leaf blade dry masses, higher essential oil yield, higher menthol levels and lower menthone levels. The results showed that Mentha can be grown in nutrient solution by reducing 65% N, 50% P, 25% K and 100% Mg. This solution had better development compared to the other tested treatments. Therefore,we recommendMentha piperita L. to be grown with such nutrient levels.

  18. Mentha spicata subsp. spicata hipokotilinden in vitro çoklu sürgün rejenerasyonu

    OpenAIRE

    Özdemir, Fethi Ahmet; Yıldırım, Mehmet Uğur; Pourali Kahriz, Mahsa

    2015-01-01

    Mentha spicata subsp. spicata tıbbi ve aromatik bir bitkidir. Bu çalışmada Mentha spicata subsp. spicata’nın in vitro’ da çoğaltımı amacıyla hipokotil eksplantları BAP ve NAA ’nın farklı kombinasyonlarını içeren, %3 sukroz ilave edilmiş ve % 0.65 agar ile katılaştırılmış MS besin ortamında kültüre alınmıştır. Araştırma sonuçları kallus oluşum yüzdesi, rejenere sürgün yüzdesi, eksplant başına düşen ortalama sürgün sayısı, ortalama sürgün uzunluğu, eksplant başına düşen ortalama kök sayısı ve o...

  19. Effects of Cultivation System and Fertilization on Seedling Production of Ocimum basilicum L. and Mentha spicata L.

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    Anastasia AKOUMIANAKI-IOANNIDOU

    2015-04-01

    Full Text Available The demand for aromatic plants as ornamentals and sources of essential oils has increased recently. A float system is a promising alternative method of seedling production. Two cropping systems, float and conventional (seed bed, and two nutrient solution formulations, one organic and one inorganic, were evaluated for Ocimum basilicum (basil and Mentha spicata (spearmint seedling production. Basil seedlings grown in float system had better or comparable growth of shoots and leaves with those grown in seedbeds, irrespective of the fertilization, whereas spearmint organic fertilization reduced the growth of the aboveground parts of the seedlings, regardless of the cropping system. By contrast, fertilization treatments on both species’ seedling grown in float system showed better root length and higher root biomass accumulation. The survival percentage of seedlings after transplanting, as well as the subsequent growth of plants, were not seriously affected by the cropping system of the fertilization method during transplant production. Overall, we conclude that a float system can be used for the production of Ocimum basilicum and Mentha spicata seedlings under organic or inorganic fertilization.

  20. Mentha piperita essential oil induces apoptosis in yeast associated with both cytosolic and mitochondrial ROS-mediated damage.

    Science.gov (United States)

    Ferreira, Patrícia; Cardoso, Teresa; Ferreira, Filipa; Fernandes-Ferreira, Manuel; Piper, Peter; Sousa, Maria João

    2014-11-01

    Mentha piperita (MP), also known as peppermint, is an aromatic and medicinal plant widely used in the food industry, perfumery and cosmetic, pharmacy and traditional medicine. Its essential oil (EO) displays antimicrobial activity against a range of bacteria and fungi. In this study, we found that MP EO lethal cytotoxicity is associated with increased levels of intracellular reactive oxygen species, mitochondrial fragmentation and chromatin condensation, without loss of the plasma membrane integrity, indicative of an apoptotic process. Overexpression of cytosolic catalase and superoxide dismutases reverted the lethal effects of the EO and of its major component menthol. Conversely, deficiency in Sod1p (cytosolic copper-zinc-superoxide dismutase) greatly increased sensitivity to both agents, but deficiency in Sod2p (mitochondrial manganese superoxide dismutase) only induced sensitivity under respiratory growth conditions. Mentha piperita EO increased the frequency of respiratory deficient mutants indicative of damage to the mitochondrial genome, although increase in mitochondrial thiol oxidation does not seem to be involved in the EO toxicity. PMID:25065265

  1. Screening of free radical scavenging compounds in water extracts of Mentha samples using a postcolumn derivatization method.

    Science.gov (United States)

    Koşar, Müberra; Dorman, H J Damien; Can Başer, K Hüsnü; Hiltunen, Raimo

    2004-08-11

    An on-line high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl (HPLC-DPPH*) method has been improved for the detection of polar and nonpolar radical scavenging compounds in complex plant extracts. Nine water extracts were prepared from different Mentha species, varieties, hybrids, and cultivars. After the components within each extract had been separated by reverse phase chromatography using 10-100% methanol with 2% acetic acid as a mobile phase, analytes within the eluent capable of scavenging a citric acid-sodium citrate-buffered methanol 1,1-diphenyl-2-picrylhydrazyl solution were detected by postcolumn derivatization at 517 nm. The HPLC-DPPH* on-line method was applied to the qualitative and quantitative analysis of Mentha extracts. There was a strong correlation between the scavenging (negative) peak area and the concentration of the radical scavenging reference substances used. The minimum detectable concentration (microg/mL) of the antioxidant compounds was determined. Caffeic acid, eriocitrin (eriodictyol-7-O-rutinoside), luteolin-7-O-glucoside, and rosmarinic acid were identified as the dominant radical scavengers in these extracts by this method. PMID:15291467

  2. Mentha piperita essential oil induces apoptosis in yeast associated with both cytosolic and mitochondrial ROS-mediated damage.

    Science.gov (United States)

    Ferreira, Patrícia; Cardoso, Teresa; Ferreira, Filipa; Fernandes-Ferreira, Manuel; Piper, Peter; Sousa, Maria João

    2014-11-01

    Mentha piperita (MP), also known as peppermint, is an aromatic and medicinal plant widely used in the food industry, perfumery and cosmetic, pharmacy and traditional medicine. Its essential oil (EO) displays antimicrobial activity against a range of bacteria and fungi. In this study, we found that MP EO lethal cytotoxicity is associated with increased levels of intracellular reactive oxygen species, mitochondrial fragmentation and chromatin condensation, without loss of the plasma membrane integrity, indicative of an apoptotic process. Overexpression of cytosolic catalase and superoxide dismutases reverted the lethal effects of the EO and of its major component menthol. Conversely, deficiency in Sod1p (cytosolic copper-zinc-superoxide dismutase) greatly increased sensitivity to both agents, but deficiency in Sod2p (mitochondrial manganese superoxide dismutase) only induced sensitivity under respiratory growth conditions. Mentha piperita EO increased the frequency of respiratory deficient mutants indicative of damage to the mitochondrial genome, although increase in mitochondrial thiol oxidation does not seem to be involved in the EO toxicity.

  3. Two-Dimensional Micro-TLC Phenolic Fingerprints of Selected Mentha sp. on Cyano-Bonded Polar Stationary Phase.

    Science.gov (United States)

    Hawrył, Mirosław A; Niemiec, Małgorzata A; Słomka, Kamil; Waksmundzka-Hajnos, Monika; Szymczak, Grażyna

    2016-01-01

    Micro-thin-layer chromatography in two-dimensional (2D-mTLC) mode in normal- (NP) and reversed-phase (RP) systems by use of cyanopropyl-bonded stationary phases was applied to make fingerprints of 11 species of Mentha genus and two finished pharmaceutical products. Non-aqueous eluents were used in the NP systems. Mixtures of acetonitrile with water and methanol with water were used in the RP chromatographic systems. Optimization of one-dimensional systems was performed by determining RM vs. composition of mobile phase dependencies for standards occurring in various Mentha sp. On the basis of these dependencies, the most selective chromatographic systems for each run were chosen. Then most selective eluents were applied to optimize two-dimensional systems by creating RF in NP systems vs. RF in RP systems correlations. The best two-dimensional systems were chosen on the basis of R(2) values for RF vs. RF correlations (the lowest values of R(2) coefficients). The 2D-mTLC optimized systems were applied to separate phenolic compounds and make fingerprints of the examined plant materials.

  4. Phytochemical Composition and Antibacterial Activity of Hydroalcoholic Extracts of Pterospartum tridentatum and Mentha pulegium against Staphylococcus aureus Isolates

    Directory of Open Access Journals (Sweden)

    Alfredo Aires

    2016-01-01

    Full Text Available Pterospartum tridentatum and Mentha pulegium are largely used in Portuguese folk medicine to treat several human disorders and inflammatory processes but without any consistent evidence for those beneficial pointed properties. Thus, the aim of the current work is to evaluate its benefits and phytochemicals related to those beneficial properties. A distinct polyphenol profile between P. tridentatum and M. pulegium was found. Taxifolin, myricetin, ginestin, ginestein, and ginestein derivatives, biochanin A-glucoside, and biochanin A were identified in P. tridentatum, whilst in M. pulegium the luteolin-7-rutinoside, diosmin, and apigenin and respective derivatives were most representative polyphenols. These variations had implications in the antiradical and antibacterial activity and the P. tridentatum exhibited the highest antibacterial activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus MSSA, which was mainly dose-dependent. This antibacterial activity seems to be related to high content of flavonols, flavones, and isoflavones, which can act synergistically with each other against this type of bacteria. Our results showed consistent evidence that Pterospartum tridentatum and Mentha pulegium are an important reservoir of phytochemicals with antiradical activity and antibacterial capacity and thus they might be used in a preventive way or in a combined pharmaceutical and antibiotic therapy against pathogenic bacteria.

  5. Phytochemical Composition and Antibacterial Activity of Hydroalcoholic Extracts of Pterospartum tridentatum and Mentha pulegium against Staphylococcus aureus Isolates.

    Science.gov (United States)

    Aires, Alfredo; Marrinhas, Eduardo; Carvalho, Rosa; Dias, Carla; Saavedra, Maria José

    2016-01-01

    Pterospartum tridentatum and Mentha pulegium are largely used in Portuguese folk medicine to treat several human disorders and inflammatory processes but without any consistent evidence for those beneficial pointed properties. Thus, the aim of the current work is to evaluate its benefits and phytochemicals related to those beneficial properties. A distinct polyphenol profile between P. tridentatum and M. pulegium was found. Taxifolin, myricetin, ginestin, ginestein, and ginestein derivatives, biochanin A-glucoside, and biochanin A were identified in P. tridentatum, whilst in M. pulegium the luteolin-7-rutinoside, diosmin, and apigenin and respective derivatives were most representative polyphenols. These variations had implications in the antiradical and antibacterial activity and the P. tridentatum exhibited the highest antibacterial activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus MSSA, which was mainly dose-dependent. This antibacterial activity seems to be related to high content of flavonols, flavones, and isoflavones, which can act synergistically with each other against this type of bacteria. Our results showed consistent evidence that Pterospartum tridentatum and Mentha pulegium are an important reservoir of phytochemicals with antiradical activity and antibacterial capacity and thus they might be used in a preventive way or in a combined pharmaceutical and antibiotic therapy against pathogenic bacteria.

  6. Evaluation of Antioxidant, Cholinesterase Inhibitory and Antimicrobial Properties of Mentha longifolia subsp. noeana and Its Secondary Metabolites

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    Abdulselam Ertaş

    2014-09-01

    Full Text Available The aim of the present study was to determine the chemical structures of the isolated compounds, the essential oil and fatty acid compositions of Mentha longifolia subsp. noeana with their biological activities. Ursolic acid (1, u vaol (2, stigmast-5-ene-3 b -yl formate (3, stigmast-5-en-3-one (4, b -sitosterol (5, bis(2-ethylhexyl benzene-1,2-dicarboxylate (6,hexacosyl (E-ferulate (7 and 5-hydroxy-6,7,3',4'-tetramethoxy flavone (8 were obtained from the aerial parts. The compounds (2-4, 6, 7 were isolated for the first time from a Mentha species. Palmitic acid (40.8% was the major component of the non-polar fraction obtained from the petroleum ether extract. Pulegone (32.3% was the main constituent of the essential oil which exhibited strong butyrylcholinesterase inhibitory activity ( 77.36 ± 0.29%, moderate antimicrobial activity against Escherichia coli , Staphylococcus aureus, and Candida albicans. The methanol extract showed 80% inhibition of lipid peroxidation, and the acetone extract possessed moderate DPPH free radical scavenging activity (60% inhibition at 100 m g/mL.

  7. Comparative study of chemistry compositions and antimicrobial potentials of essential oils and oleoresins from dried and fresh Mentha longifolia L.

    Directory of Open Access Journals (Sweden)

    Sunita Singh

    2015-12-01

    Full Text Available Objective: To investigate the chemical compositions and antimicrobial potentials of the essential oils and oleoresins obtained from fresh and dried Mentha longifolia L. Methods: Gas chromatography and gas chromatography-mass spectrometer techniques were used to determine the profiling of the essential oils and oleoresins. In order to determine the antimicrobial efficacy of the volatile oil and oleoresins, the pathogenic fungus Aspergillus niger (1884, Aspergillus flavus (2479, Fusarium monoliforme (1893, Fusarium graminearum (2088 and Penicillium viridicatum (2007 were undertaken whereas four pathogenic bacteria Bacillus subtilis (1790, Staphylococcus aureus (3103 (Gram-positive, Escherichia coli (1672, Pseudomonas aeruginosa (1942 (Gram-negative were selected for the present study. Food poisoned, inverted Petri plate, agar well diffusion and disk diffusion methods were employed for investigating antimicrobial potentials. Results: Piperitenone oxide, an oxygenated monoterpene, dominated the chemical compositions of essential oils and oleoresins whose compositions varied from 23.5%–87.8%. Both essential oils showed good antifungal activities against Aspergillus and Fusarium species. The antibacterial investigations revealed that Gram-positive bacteria were more sensitive to the essential oils. Conclusions: Drying the fresh herbal materials influences the chemical contents and the biological activities of the essential oils and oleoresins. Such results indicate that essential oils of Mentha longifolia L. can be possible candidates for further investigations to isolate and characterize their active principles as possible new natural preservatives.

  8. Phytochemical Composition and Antibacterial Activity of Hydroalcoholic Extracts of Pterospartum tridentatum and Mentha pulegium against Staphylococcus aureus Isolates.

    Science.gov (United States)

    Aires, Alfredo; Marrinhas, Eduardo; Carvalho, Rosa; Dias, Carla; Saavedra, Maria José

    2016-01-01

    Pterospartum tridentatum and Mentha pulegium are largely used in Portuguese folk medicine to treat several human disorders and inflammatory processes but without any consistent evidence for those beneficial pointed properties. Thus, the aim of the current work is to evaluate its benefits and phytochemicals related to those beneficial properties. A distinct polyphenol profile between P. tridentatum and M. pulegium was found. Taxifolin, myricetin, ginestin, ginestein, and ginestein derivatives, biochanin A-glucoside, and biochanin A were identified in P. tridentatum, whilst in M. pulegium the luteolin-7-rutinoside, diosmin, and apigenin and respective derivatives were most representative polyphenols. These variations had implications in the antiradical and antibacterial activity and the P. tridentatum exhibited the highest antibacterial activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus MSSA, which was mainly dose-dependent. This antibacterial activity seems to be related to high content of flavonols, flavones, and isoflavones, which can act synergistically with each other against this type of bacteria. Our results showed consistent evidence that Pterospartum tridentatum and Mentha pulegium are an important reservoir of phytochemicals with antiradical activity and antibacterial capacity and thus they might be used in a preventive way or in a combined pharmaceutical and antibiotic therapy against pathogenic bacteria. PMID:27190990

  9. Two-Dimensional Micro-TLC Phenolic Fingerprints of Selected Mentha sp. on Cyano-Bonded Polar Stationary Phase.

    Science.gov (United States)

    Hawrył, Mirosław A; Niemiec, Małgorzata A; Słomka, Kamil; Waksmundzka-Hajnos, Monika; Szymczak, Grażyna

    2016-01-01

    Micro-thin-layer chromatography in two-dimensional (2D-mTLC) mode in normal- (NP) and reversed-phase (RP) systems by use of cyanopropyl-bonded stationary phases was applied to make fingerprints of 11 species of Mentha genus and two finished pharmaceutical products. Non-aqueous eluents were used in the NP systems. Mixtures of acetonitrile with water and methanol with water were used in the RP chromatographic systems. Optimization of one-dimensional systems was performed by determining RM vs. composition of mobile phase dependencies for standards occurring in various Mentha sp. On the basis of these dependencies, the most selective chromatographic systems for each run were chosen. Then most selective eluents were applied to optimize two-dimensional systems by creating RF in NP systems vs. RF in RP systems correlations. The best two-dimensional systems were chosen on the basis of R(2) values for RF vs. RF correlations (the lowest values of R(2) coefficients). The 2D-mTLC optimized systems were applied to separate phenolic compounds and make fingerprints of the examined plant materials. PMID:26673190

  10. Organic Extractives from Mentha spp. Honey and the Bee-Stomach: Methyl Syringate, Vomifoliol, Terpenediol I, Hotrienol and Other Compounds

    Directory of Open Access Journals (Sweden)

    Igor Jerković

    2010-04-01

    Full Text Available The GC and GC/MS analyses of the solvent organic extractive from the stomach of the bees, having collected Mentha spp. nectar, revealed the presence of methyl syringate (6.6%, terpendiol I (5.0% and vomifoliol (3.0% that can be attributed to the plant origin. Other major compounds from the bee-stomach were related to the composition of cuticular waxes and less to pheromones. Organic extractivesfrom Mentha spp. honey were obtained by solvent-free headspace solid-phase microextraction (HS-SPME and ultrasonic solvent extraction (USE and analyzed by GC and GC/MS. The major honey headspace compounds were hotrienol (31.1%–38.5%, 2-methoxy-4-methylphenol (0.5–6.0%, cis- and trans-linalool oxides (0.9–2.8%, linalool (1.0–3.1% and neroloxide (0.9–1.9%. Methyl syringate was the most abundant compound (38.3-56.2% in the honey solvent extractives followed by vomifoliol (7.0–26.6%. Comparison of the honey organic extractives with the corresponding bee-stomach extractive indicated that methyl syringate and vomofoliol were transferred to the honey while terpendiol I was partially transformed to hotrienol in ripened honey.

  11. Development and essential oil yield of mint (Mentha x piperita L. cultivated in nutritive solution under different levels of phosphorus

    Directory of Open Access Journals (Sweden)

    Evelize de Fátima Saraiva David

    2007-06-01

    Full Text Available To evaluate the development and essential oil yield of Mentha x piperita L, plants were cultivated using complete Hoagland and Arnon (1950 no 2 solution with a 50% increase or decrease in the phosphorus (P concentration. The following were evaluated: Leaf number, leaf area, stem length, dry mass of several organs, total dry mass, leaf area ratio (LAR, leaf mass ratio (RMF, net assimilatory rate (NAR, relative growth rate (RGR, specific leaf area (SLA, dry mass distribution, and essential oil yield. The experimental design was completely randomized in a factorial scheme 3x5, with three levels and five crops, each with four replications. For essential oil, a randomized 3x3 factorial design was used. Data analysis consisted in application of ANOVA and regression analysis, using a 5% level of significance. The results led to the conclusion that the levels of phosphorus affect the development of Mentha x piperita L. Although plants showed variations in several of the parameters evaluated when submitted to different levels of phosphorus, they adjusted to these conditions, as demonstrated by the relative growth rate (RGR. However, the plants cultivated with lower levels of phosphorus resulted in a higher essential oil yield at 60 DAT.

  12. Identification of the essential oils composition from four ecotypes of Mentha longifolia (L. Huds. growing wild in Isfahan province, Iran

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    REZA ABEDI

    2015-08-01

    Full Text Available Mentha longifolia L., commonly known as wild mint, belongs to family Lamiaceae. The aim of this study was to identify the chemical composition of essential oil extracted from four ecotypes of Mentha longifolia L. grown wild (Shahreza, Chadegan, Isfahan, and Falavarjan in Isfahan province (Central Iran. The essential oil was extracted by a Clevenger approach and analyzed using GC/MS. In the aerial parts of the plant were identified 26, 30, 22 and 25 compounds for Shahreza, Chadegan, Isfahan and Falavarjan ecotypes, respectively. The major constituents of the essential oil from the aerial parts of M. longifolia in Shahreza province were piperitenone oxide (26.71%, 1,8-cineole (20.72%, α-pinene (14.28%, pulegone (7.81%, sabinene (7.06% and trans-caryophyllene (4.23%. The main compositions in Chadegan province were piperitenone oxide (29.13%, 1,8-cineole (28.84%, sabinene (9.05%, pulegone (8.97% and α-pinene (6.31%. The main compositions in Isfahan province were pulegone (44.75%, 1,8-cineole (13.82%, 2-cyclohexen-1-ol, 1-methyl (8.49%, isopulegone (8.07% and menthone (4.37%. In Falavarjan province the constituents were pulegone (33.39%, 1,8-cineole (29.79%, sabinene (11.23% and isopulegone (7.28%.

  13. Antimicrobial properties of plant extracts of Thymus vulgaris L., Ziziphora tenuior L. and Mentha Spicata L., against important foodborne pathogens in vitro

    Directory of Open Access Journals (Sweden)

    Faride Tabatabaee

    2013-03-01

    Full Text Available The ancient-Iranians were familiar with many medicinal herbs and were aware of their usefulness in treatment of various diseases. In this study Thymus vulgaris L., Ziziphora tenuior L. and Mentha Spicata L. extracted with methanol 96°and the antimicrobial effects of extracts were evaluated on Escherichia coli PTCC 1330 and Staphylococcus aureus PTCC 1337 by “using the method of Collins” and “disk agar diffusion method”. The results show that methanolic extracts were quite effective in 2000 μg/ml concentration on Escherichia coli PTCC 1330 and Staphylococcus aureus PTCC 1337 and were prevented from growth them on medium. In “disk agar diffusion method”, 10, 20, 30 and 40 % methanolic extract concentrations, was deterrented effect on Escherichia coli PTCC 1330 and Staphylococcus aureus PTCC 1337. The Thymus vulgaris L., Ziziphora tenuior L. and Mentha Spicata L. extracts presented the more effective impact on the growth of Staphylococcus aureus PTCC 1337than Escherichia coli PTCC 1330 (pE. coli was the resistant bacterium against Thymus vulgaris L., Ziziphora tenuior L. and Mentha Spicata L.  extracts E. coli is known to possess a high level of intrinsic resistance to most of the antimicrobial agents due to a very restrictive outer membrane barrier. As a result alcoholic extracts of Thymus vulgaris L., Ziziphora tenuior L. and Mentha Spicata L., have been strong antimicrobial activity against many food pathogen bacteria. Results showed Thymus vulgaris L., Ziziphora tenuior L. and Mentha Spicata L. extracts can be used as natural antimicrobial in food products.

  14. Actividad Antifúngica de Aceites Esenciales de Canela (Cinnamomum zeylanicum Blume) y Orégano (Origanum vulgare L.) y su Efecto sobre la Producción de Aflatoxinas en Nuez Pecanera [Carya illinoensis (F.A. Wangenh) K. Koch

    OpenAIRE

    Erick Arturo García-Camarillo; Martha Yolanda Quezada-Viay; Josefina Moreno-Lara; Gabriela Sánchez-Hernández; Ernesto Moreno-Martínez; María Cristina Julia Pérez-Reyes

    2006-01-01

    Los aceites esenciales de canela (Cinnamomum zeylanicum) y orégano (Origanum vulgare) fueron evaluados para determinar su actividad antifúngica contra Aspergillus flavus y la producción de aflatoxinas en nuez pecanera. Ambos aceites presentaron actividad fungicida in vitro contra A. flavus, el aceite esencial de orégano a partir de 1000 ppm y el de canela de 2000 ppm, en medio de cultivo de malta-salagar y un efecto fungistático en 100 ppm. Sin embargo, al evaluar el efecto inhibitorio en la ...

  15. Reguladores de crescimento vegetal no cultivo in vitro de Mentha x Piperita L.

    Directory of Open Access Journals (Sweden)

    T.P. Morais

    2014-01-01

    Full Text Available A micropropagação é uma técnica muitas vezes indicada para a multiplicação em larga escala de plantas com propriedades medicinais. Dentre elas, destaca-se a hortelã-pimenta (Mentha x Piperita L., cujo óleo essencial é utilizado no tratamento de transtornos digestivos e respiratórios. Para otimizar o protocolo de micropropagação dessa espécie são necessários estudos, principalmente quanto à suplementação do meio de cultura para garantir a produção massal in vitro e posterior extração do óleo essencial. Nesse contexto, objetivou-se avaliar o efeito de concentrações e combinações de reguladores de crescimento vegetal na morfogênese in vitro de hortelã-pimenta. Segmentos nodais provenientes de plântulas estabelecidas in vitro foram utilizados como fonte de explante e inoculados em meio de cultura MS suplementado com 0; 2,0 e 4,0 mg L-1 de BAP (6-benzilaminopurina, 0; 0,5 e 1,5 mg L-1 de ANA (ácido naftaleno-acético e 0; 0,5 e 1,0 mg L-1 de GA3 (ácido giberélico. O delineamento experimental adotado foi inteiramente casualizado, com os tratamentos distribuídos em esquema fatorial 3x3x3 com oito repetições. Concluiu-se que o BAP favoreceu a sobrevivência de segmentos nodais de M. x Piperita inoculados in vitro e, quando combinado ao GA3, promoveu a brotação dos explantes. Essas características, no entanto, não foram estimuladas pela adição de ANA ao meio de cultura. Conclui-se que após a multiplicação dos brotos in vitro estes devem ser transferidos para meio sem reguladores para seu desenvolvimento. Apesar dos efeitos benéficos do BAP na organogênese de M. x Piperita, elevadas concentrações deste regulador de crescimento vegetal promoveram a formação de calos.

  16. Preliminary study of the antimicrobial activity of Mentha x villosa Hudson essential oil, rotundifolone and its analogues Estudo preliminar da atividade antimicrobiana do óleo essencial de Mentha x villosa Hudson, rotundifolona e seus análogos

    Directory of Open Access Journals (Sweden)

    Thúlio. A. Arruda

    2006-09-01

    Full Text Available Essential oils present antimicrobial activity against a variety of bacteria and yeasts, including species resistant to antibiotics and antifungicals. In this context, this work aims at the evaluation of the antimicrobial activity of the essential oil of Mentha x villosa Hudson ("hortelã da folha miúda", its major component (rotundifolone and four similar analogues of rotundifolone (limonene oxide, pulegone oxide, carvone epoxide and (+-pulegone against strain standards of Staphylococcus aureus ATCC 25923, E. coli ATCC 25922, Pseudomona aeruginosa ATCC 27853, Candida albicans ATCC 76645 and one strain of meticilin - resistant Staphylococcus aureus - MRSA (171c from human clinic. The method of the diffusion in plates with solid medium was used. The results showed that the oil of Mentha x villosa, rotundifolone, limonene oxide and (+-pulegone, are similar regarding the antimicrobial activity against the tested strains of S. aureus and C. albicans. All of the products present antimocrobial potential with antibacterial activity for S. aureus ATCC 25923 and antifungal activity for C. albicans ATCC 76645. None of the products presented antimicrobial activity for the strains of E. coli ATCC 25922 and P. aeruginosa ATCC 27853, representatives of the Gram negative bacteria.Os óleos essenciais apresentam atividade antimicrobiana contra uma variedade de bactérias e fungos, incluindo espécies resistentes a antibióticos e antimicóticos. Neste contexto, este trabalho objetiva a avaliação da atividade de antimicrobiana do óleo essencial de Mentha x villosa Hudson (hortelã-da-folha-miúda - seu componente majoritário (rotundifolona e quatro análogos sintéticos da rotundifolona (epóxi-limoneno epóxi-pulegona, epóxi-carvona e (+-pulegona frente a cepas padrão de Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 76645 e uma cepa de Staphylococcus aureus meticilina - resistente

  17. Flavor retention of peppermint (Mentha piperita L.) essential oil spray-dried in modified starches during encapsulation and storage

    DEFF Research Database (Denmark)

    Baranauskiene, R.; Bylaite, Egle; Zukauskaite, J.;

    2007-01-01

    The effect of different commercial modified food starch carrier materials on the flavor retention of the essential oil (EO) of peppermint (Mentha piperita L.) during spray drying and storage was evaluated. The obtained results revealed that the emulsification and encapsulation efficiencies...... of peppermint EO were higher for all n-octenyl succinic anhydride (OSAN)-modified starches as compared to those of hydrolyzed starches (dextrins). The compositions of pure, emulsified, and encapsulated peppermint EOs in different matrices were quite similar; however, some changes in the percentages of some...... individual compounds were observed. Larger differences in the compositions of surface oils from various encapsulation products were obtained. Flavor components were released at different rates by each of the encapsulated products. The aroma binding capacity of different modified starch matrices to lock EO...

  18. Flavor retention of peppermint (Mentha piperita L.) essential oil spray-dried in modified starches during encapsulation and storage.

    Science.gov (United States)

    Baranauskiene, Renata; Bylaite, Egle; Zukauskaite, Jurate; Venskutonis, Rimantas P

    2007-04-18

    The effect of different commercial modified food starch carrier materials on the flavor retention of the essential oil (EO) of peppermint (Mentha piperita L.) during spray drying and storage was evaluated. The obtained results revealed that the emulsification and encapsulation efficiencies of peppermint EO were higher for all n-octenyl succinic anhydride (OSAN)-modified starches as compared to those of hydrolyzed starches (dextrins). The compositions of pure, emulsified, and encapsulated peppermint EOs in different matrices were quite similar; however, some changes in the percentages of some individual compounds were observed. Larger differences in the compositions of surface oils from various encapsulation products were obtained. Flavor components were released at different rates by each of the encapsulated products. The aroma binding capacity of different modified starch matrices to lock EO droplets depends on the water activity, and the leakage of aromas from encapsulated powder products during storage increased with increasing water activity. PMID:17381102

  19. Multidisciplinary Approach to Determine the Optimal Time and Period for Extracting the Essential Oil from Mentha suaveolens Ehrh

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    Stefania Garzoli

    2015-05-01

    Full Text Available A comprehensive study on essential oils (EOs extracted from some Mentha suaveolens L. samples, collected in the countryside of Tarquinia, is reported. In this study, the procedure for essential oil preparation, in terms of harvesting and extraction time, was analyzed in detail for the first time. The GC/MS analysis, carried out on 18 samples, revealed that piperitenone oxide (PO, the main essential oils’ chemical constituent, is primarily responsible for the related antifungal activity. Nevertheless, EOs with lower PO content indicate that other chemicals, such as para-cymenene, may participate in exerting the EOs’ antifungal effect. Furthermore, the bacterial reverse mutation assay highlighted lack of mutagenic effect in all tested samples. Analysis of the results indicated that for higher activity, the essential oils should be produced with 3 h maximum hydrodistillation, regardless of the harvesting time. Differently, the maximum essential oil yield can be obtained in August and the highest piperitenone oxide percentage is obtainable in July.

  20. Chemical characterization and bioactive properties of two aromatic plants: Calendula officinalis L. (flowers) and Mentha cervina L. (leaves).

    Science.gov (United States)

    Miguel, María; Barros, Lillian; Pereira, Carla; Calhelha, Ricardo C; Garcia, Pablo A; Castro, MaÁngeles; Santos-Buelga, Celestino; Ferreira, Isabel C F R

    2016-05-18

    The chemical composition and bioactive properties of two plants (Calendula officinalis L. and Mentha cervina L.) were studied. Their nutritional value revealed a high content of carbohydrates and low fat levels, and very similar energy values. However, they presented different profiles in phenolic compounds and fatty acids; C. officinalis presented mainly glycosylated flavonols and saturated fatty acids, while M. cervina presented mainly caffeoyl derivatives and polyunsaturated fatty acids. M. cervina showed the highest concentration of phenolic compounds while C. officinalis presented higher amounts of sugars, organic acids and tocopherols. The highest antioxidant and cytotoxic activities were obtained for the hydromethanolic extract of M. cervina, which presented the lowest values of EC50 and exhibited cytotoxicity against the four tumor cell lines tested. Infusions showed no cytotoxicity for the tumor cell lines, and none of the extracts showed toxicity against non-tumor cells. This study contributes to expand the knowledge on both natural sources and therefore their use. PMID:27110832

  1. 薄荷属植物染色体观察%Observation on chromosome of Mentha L.

    Institute of Scientific and Technical Information of China (English)

    于盱; 梁呈元; 李维林; 刘艳

    2009-01-01

    对薄荷属植物3个种类及薄荷(Mentha haplocalyx Briq.)的4个栽培品种的染色体进行了观察.结果表明,薄荷属植物染色体基数x=12,染色体大小为0.43~1.93 μm,多倍化现象非常普遍.其中椒样薄荷(M. piperita Linn.)2n=6x=72,留兰香(M. spicata Linn.)2n=8x=96,圆叶薄荷(M. rotundifolia Huds.)2n=3x=36,薄荷品种39、687和Fu-1 2n=6x=96,品种738为非整倍体,2n=90.

  2. Evaluation of drying methods with respect to drying parameters, some nutritional and colour characteristics of peppermint (Mentha x piperita L.)

    Energy Technology Data Exchange (ETDEWEB)

    Arslan, Derya; Oezcan, M. Musa [Selcuk University, Faculty of Agriculture, Department of Food Engineering, Konya (Turkey); Menges, Hakan Okyay [Selcuk University, Faculty of Agriculture, Department of Farm Machinery, Konya (Turkey)

    2010-12-15

    Peppermint leaves (Mentha x piperita L.) were dried by using sun, oven (50 C) and microwave oven (700 W) drying methods. Page, Modified page, Midilli and Kuecuek models adequately described the oven, sun and microwave oven drying behaviours of peppermint leaves. The drying process was explicated through the diffusional model in order to obtain effective diffusivity values, which were determined as 3.10 x 10{sup -12}, 2.68 x 10{sup -12} and 4.09 x 10{sup -10} for the sun, oven and microwave oven drying process, respectively. Fresh and dried herbs had high amounts of K, Ca, P, Mg, Fe and Al minerals. Microwave oven drying method leaded to the lowest increase in Ag, Al, B, Na, Mn, Mg and Zn values than the other drying methods. Microwave oven drying shortened the drying time, revealed the highest phenolic content and optimum colour values. (author)

  3. Extraction, preliminary characterization and evaluation of in vitro antitumor and antioxidant activities of polysaccharides from Mentha piperita.

    Science.gov (United States)

    Liu, Xin; Sun, Zhen-Liang; Jia, Ai-Rong; Shi, Ya-Ping; Li, Rui-Hong; Yang, Pei-Ming

    2014-01-01

    This study describes the extraction, preliminary characterization and evaluation of the in vitro antitumor and antioxidant activities of polysaccharides extracted from Mentha piperita (MPP). The optimal parameters for the extraction of MPP were obtained by Box-Behnken experimental design and response surface methodology (RSM) at the ratio of water to raw material of 20, extraction time of 1.5 h and extraction temperature at 80 °C. Chemical composition analysis showed that MPP was mainly composed of glucuronic acid, galacturonic acid, glucose, galactose and arabinose, and the molecular weight of its two major fractions were estimated to be about 2.843 and 1.139 kDa, respectively. In vitro bioactivity experiments showed that MPP not only inhibited the growth of A549 cells but possessed potent inhibitory action against DNA topoisomerase I (topo I), and an appreciative antioxidant action as well. These results indicate that MPP may be useful for developing safe natural health products.

  4. Multidisciplinary Approach to Determine the Optimal Time and Period for Extracting the Essential Oil from Mentha suaveolens Ehrh.

    Science.gov (United States)

    Garzoli, Stefania; Pirolli, Adele; Vavala, Elisabetta; Di Sotto, Antonella; Sartorelli, Gianni; Božović, Mijat; Angiolella, Letizia; Mazzanti, Gabriela; Pepi, Federico; Ragno, Rino

    2015-01-01

    A comprehensive study on essential oils (EOs) extracted from some Mentha suaveolens L. samples, collected in the countryside of Tarquinia, is reported. In this study, the procedure for essential oil preparation, in terms of harvesting and extraction time, was analyzed in detail for the first time. The GC/MS analysis, carried out on 18 samples, revealed that piperitenone oxide (PO), the main essential oils' chemical constituent, is primarily responsible for the related antifungal activity. Nevertheless, EOs with lower PO content indicate that other chemicals, such as para-cymenene, may participate in exerting the EOs' antifungal effect. Furthermore, the bacterial reverse mutation assay highlighted lack of mutagenic effect in all tested samples. Analysis of the results indicated that for higher activity, the essential oils should be produced with 3 h maximum hydrodistillation, regardless of the harvesting time. Differently, the maximum essential oil yield can be obtained in August and the highest piperitenone oxide percentage is obtainable in July.

  5. Effect of gamma irradiation on chemical composition, antimicrobial and antioxidant activities of Thymus vulgaris and Mentha pulegium essential oils

    Science.gov (United States)

    Zantar, Said; Haouzi, Rachid; Chabbi, Mohamed; Laglaoui, Amin; Mouhib, Mohammed; Mohammed Boujnah; Bakkali, Mohammed; Zerrouk, Mounir Hassani

    2015-10-01

    The effects of gamma irradiation doses (10, 20 and 30 kGy) on chemical composition, antimicrobial and antioxidant activities of Thymus vulgaris and Mentha pulegium essential oils (EOs) have been studied. The chromatographic analysis showed that the studied EOs were constituted mainly by carvacrol for T. vulgaris and pulegone for M. pulegium. Gamma irradiation on the studied doses, affects quantitatively and not qualitatively some components of the investigated oils. This effect was dose dependent. While the antioxidant activity remains stable at any dose applied for the plants studied, the antimicrobial activity increased in the irradiated samples for gram negative bacteria and did not change for gram+bacteria. This study supports that gamma irradiation employed at sterilizing doses did not compromise the biological activities of medicinal and aromatic plants.

  6. COMPARATIVE ACCOUNT ON GC-MS ANALYSIS OF MENTHA ARVENSIS L. CORN MINT FROM THREE DIFFERENT LOCATIONS OF NORTH INDIA

    Directory of Open Access Journals (Sweden)

    SHARMA VIVEK1*, SHARMA NISHA1, SINGH HARBANS 1, SRIVASTAVA K. DEVENDRA1, PATHANIA VIJAYLATA2, SINGH BIKRAM2, GUPTA C. RAGHBIR1

    2009-12-01

    Full Text Available The essential oil analysis of Mentha arvensis L. (Corn Mint has been done for the first time from three locations of NorthIndia. The extraction yields for the essential oils of three locations of M. arvensis were: 0.38% for sample M-1 collectedfrom Fatehpur (415m, 0.31% for sample M-2 from Dhameta (435m and 0.36% for sample MP from Patiala (250m. Theoils were analyzed by GC-MS, the components of oil were identified by comparing their retention indices and mass spectrafragmentation patterns with those stored on the MS-computer library and also from the published literatures. The majorconstituents reported from essential oils of M. arvensis were: L-Menthone, Menthol, Isomenthone, Eucalyptol, Piperitoneoxide, Carvone, dl-Limonene, trans-Dihydrocarvone, Germacrene-D, etc. from all three samples collected from Punjab andHimachal Pradesh of Northern India.

  7. Comparison between the essential oil and supercritical Carbon Dioxide extraction of Mentha Piperita L. cultivated in Iran

    Directory of Open Access Journals (Sweden)

    Aghel N

    2002-08-01

    Full Text Available The chemical compositions of the essential oils and the volatile concentrate of Mentha piperita L. (Labiatae obtained by supercritical carbon dioxide extraction (SFE at 35°C and 100 atm were compared using GC/MS. Whereas twenty four components were identified in the essential oil, only seven compounds, including the main compounds of the peppermint oil were isolated by the SFE. The percent of major components of the oil and the extract were: Menthol (31.53 and 48.39, Menthone (23.37 and 26.68 and Isomenthone (11.11 and 6.58, respectively. From these results it may concluded that the SFE method supply a selective essential oil extract.

  8. Flavor retention of peppermint (Mentha piperita L.) essential oil spray-dried in modified starches during encapsulation and storage.

    Science.gov (United States)

    Baranauskiene, Renata; Bylaite, Egle; Zukauskaite, Jurate; Venskutonis, Rimantas P

    2007-04-18

    The effect of different commercial modified food starch carrier materials on the flavor retention of the essential oil (EO) of peppermint (Mentha piperita L.) during spray drying and storage was evaluated. The obtained results revealed that the emulsification and encapsulation efficiencies of peppermint EO were higher for all n-octenyl succinic anhydride (OSAN)-modified starches as compared to those of hydrolyzed starches (dextrins). The compositions of pure, emulsified, and encapsulated peppermint EOs in different matrices were quite similar; however, some changes in the percentages of some individual compounds were observed. Larger differences in the compositions of surface oils from various encapsulation products were obtained. Flavor components were released at different rates by each of the encapsulated products. The aroma binding capacity of different modified starch matrices to lock EO droplets depends on the water activity, and the leakage of aromas from encapsulated powder products during storage increased with increasing water activity.

  9. Triterpenóides pentacíclicos de Mentha villosa: identificação estrutural e atribuição dos deslocamentos químicos dos átomos de hidrogênio e carbono Pentacyclic triterpenoids of Mentha villosa: structural identification and ¹H and 13C resonance assignments

    OpenAIRE

    Francisco J. Queiroz Monte; Eliete F. de Oliveira; Raimundo Braz Filho

    2001-01-01

    The structures of seven oleanene and ursene triterpenoids (1-7) isolated from aerial parts of Mentha villosa were identified. In addition, the complete ¹H and 13C resonance assignments of these triterpenoids were accomplished using 1D and 2D NMR spectroscopic experiments.

  10. Modulatory Effects of Mentha spicata (Linn. against 4 Nitroquinoline-1- Oxide Induced Chromosome Damage and Oxidative Stress in Mice

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    Ponnan Arumugam

    2015-12-01

    Full Text Available Mentha spicata is a common medicinal and edible plant in India and used in the ayurvedic system of medicine to treat various ailments including as a memory enhancer. The present study is aimed to evaluate the modulatory effects of Mentha spicata (Linn. against 4?nitroquinoline- 1-oxide (4-NQO induced chromosome damage and oxidative stress in mice. Experiments were conducted with eight groups of either sex of mice. Ethanol extract (EE of three doses (80, 160 and 320 mg/kg body weight-bw with or without 4-NQO along with vehicle control (25% DMSO in water were administered orally for five consecutive days. 4-NQO (7.5 mg/kg bw was injected intraperitoneally on the sixth day, and the animals scarified the following day. 4-NQO enhanced the frequency of micronucleated polychromatic erythrocytes (MnPCEs by about 4.2 times the control value, 15.78 MnPCEs/2500 PCEs. Pretreatment with EE, significantly reduced the MnPCEs frequency (50-69% induced by 4-NQO. Moreover, 4-NQO enhanced the lipid peroxidation (LPO by about 60% with decrease of enzymatic antioxidants in the range 27-41% and 38-60% for nonenzymatic antioxidants over the respective controls. Pre-treatment with the EE, brought down 4-NQO induced LPO significantly with in a dose dependent manner. All the measured antioxidants are positively modulated by the EE. The modulated values at high doses are either comparable with the controls or even higher than the control values.

  11. Study Progress in Tissue and Cell Culture of Mentha haplocalyx Briq%薄荷组织和细胞培养的研究进展

    Institute of Scientific and Technical Information of China (English)

    张泽宏; 王兵丽; 陈巧玲

    2013-01-01

    This paper introduced the domestic research situation in tissue and cell culture of Mentha haplocalyx Briq. with the related data, and pointed out the limitation and shortcoming of tissue culture technology, and direct the next step of study in tissue and cell culture of Mentha haplocalyx Briq..%结合相关资料,综述了薄荷组织的离体培养和细胞悬浮培养的研究概况,指出目前薄荷组培技术方面的局限和不足,提出细胞培养直接产生次生代谢产物的开发利用途径,为以后进行薄荷组织和细胞培养工作指明方向。

  12. Organization of Monoterpene Biosynthesis in Mentha. Immunocytochemical Localizations of Geranyl Diphosphate Synthase, Limonene-6-Hydroxylase, Isopiperitenol Dehydrogenase, and Pulegone Reductase1

    Science.gov (United States)

    Turner, Glenn W.; Croteau, Rodney

    2004-01-01

    We present immunocytochemical localizations of four enzymes involved in p-menthane monoterpene biosynthesis in mint: the large and small subunits of peppermint (Mentha x piperita) geranyl diphosphate synthase, spearmint (Mentha spicata) (−)-(4S)-limonene-6-hydroxylase, peppermint (−)-trans-isopiperitenol dehydrogenase, and peppermint (+)-pulegone reductase. All were localized to the secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development. Immunogold labeling of geranyl diphosphate synthase occurred within secretory cell leucoplasts, (−)-4S-limonene-6-hydroxylase labeling was associated with gland cell endoplasmic reticulum, (−)-trans-isopiperitenol dehydrogenase labeling was restricted to secretory cell mitochondria, while (+)-pulegone reductase labeling occurred only in secretory cell cytoplasm. We discuss this pathway compartmentalization in relation to possible mechanisms for the intracellular movement of monoterpene metabolites, and for monoterpene secretion into the extracellular essential oil storage cavity. PMID:15542490

  13. Protective effects of solvent fractions of Mentha spicata (L.) leaves evaluated on 4-nitroquinoline-1-oxide induced chromosome damage and apoptosis in mouse bone marrow cells

    OpenAIRE

    Ponnan Arumugam; Arabandi Ramesh

    2009-01-01

    Spearmint leaves (Mentha spicata L.) contain high levels of antioxidants that are known to protect against both exogenous and endogenous DNA damage. In this study, the protective effects of the hexane fraction (HF), chloroform fraction (CF) and ethyl acetate fraction (EAF) in an ethanol extract from M. spicata were evaluated against 4-nitroquinoline-1-oxide (4-NQO) induced chromosome damage and apoptosis in bone marrow cells of Swiss albino mice. Two (EAF; 80 and 160 mg/ kg body weight - bw) ...

  14. Mentha spicata var. spicata (L. 1753) and Raphanus sativus var. sativus (L. 1753) : survival meal for Medauroidea extradentata (Brunner von Wattenwyl 1907) (Phasmatodea: Phasmatidae)

    OpenAIRE

    Olive, Gabriel; Zimmer, Jean-Yves; OLIVE, Gilles

    2016-01-01

    La version française de l'article est hal-01277035. International audience When food shortages for a stick insect breeding of the species Medauroidea extradentata, feeding trials were conducted with spearmint (Mentha spicata var. spicata). Contrary to what was expected, the stems were eaten more than the leaves. In addition, four specimens of this species were found after surviving without water or food other than radish plants (Raphanus sativus var. sativus). So it seems that M. extrad...

  15. Effects of Cultivation System and Fertilization on Seedling Production of Ocimum basilicum L. and Mentha spicata L.

    OpenAIRE

    Anastasia AKOUMIANAKI-IOANNIDOU; Marina RASOULI; Lamprini PODAROPOULOU; Ioannis KARAPANOS; Dimitrios BILALIS

    2015-01-01

    The demand for aromatic plants as ornamentals and sources of essential oils has increased recently. A float system is a promising alternative method of seedling production. Two cropping systems, float and conventional (seed bed), and two nutrient solution formulations, one organic and one inorganic, were evaluated for Ocimum basilicum (basil) and Mentha spicata (spearmint) seedling production. Basil seedlings grown in float system had better or comparable growth of shoots and leaves with thos...

  16. Evaluation of possible toxic effects of spearmint (Mentha spicata) on the reproductive system, fertility and number of offspring in adult male rats

    OpenAIRE

    Fatemeh Nozhat; Sanaz Alaee; Khodabakhsh Behzadi; Najmeh Azadi Chegini

    2014-01-01

    Objective: In this study we investigated the effects of spearmint (Mentha spicata Labiatae) on the reproductive system, fertility and number of offspring in adult male rats. Materials and Methods: Adult Wistar male rats in one control (C) and three experimental groups (I, II and III) received 0, 10, 20 and 40 mg/kg spearmint extract orally for 45 days, respectively.  Following this treatment, the animals’ weights, and the standard weight of reproductive tissues, sperm count, sperm motility an...

  17. Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram negative bacteria

    OpenAIRE

    Fohad Mabood Husain; Iqbal eAhmad; Mohammad Shavez Khan; Ejaz eAhmad; Mohammad Shahnawaz Khan; Qudsia eTahseen; Nasser Abdul latif Alshabib

    2015-01-01

    Bacterial quorum sensing (QS) is a density dependent communication system that regulates the expression of certain genes including production of virulence factors in many pathogens. Bioactive plant extract/compounds inhibiting QS regulated gene expression may be a potential candidate as antipathogenic drug. In this study anti-QS activity of peppermint (Mentha piperita) oil was first tested using the Chromobacterium violaceum CVO26 biosensor. Further, the findings of the present investigatio...

  18. Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria

    OpenAIRE

    Husain, Fohad M.; Ahmad, Iqbal; Khan, Mohammad S.; Ahmad, Ejaz; Tahseen, Qudisa; Khan, Mohd Shahnawaz; Alshabib, Nasser A.

    2015-01-01

    Bacterial quorum sensing (QS) is a density dependent communication system that regulates the expression of certain genes including production of virulence factors in many pathogens. Bioactive plant extract/compounds inhibiting QS regulated gene expression may be a potential candidate as antipathogenic drug. In this study anti-QS activity of peppermint (Mentha piperita) oil was first tested using the Chromobacterium violaceum CVO26 biosensor. Further, the findings of the present investigation ...

  19. Effect of Different Planting Times on Yields and Agricultural Characters of Different Mint (Mentha spp.) Varieties Under The Harran Plain Conditions

    OpenAIRE

    ÖZEL, Abdulhabip

    1998-01-01

    This study was conducted to determine the effect of different planting times on yield and characters of different mint varieties (Mentha arvensis var. piperascens, M. piperita Mitcham, M. piperita Eskişehir Nanesi and M. piperita Prilubskaja). The field trials were carried out at GAP Agricultural Research Station of Çukurova University, Faculty of Agriculture in the Harran Plain conditions in years of 1993 and 1994. Autumn transplants gave higher herb yields and essential oil contents than pl...

  20. Effect of three 2-allyl-p-mentha-6,8-dien-2-ols on inhibition of mild steel corrosion in 1 M HCl

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    S. Kharchouf

    2014-11-01

    Full Text Available 2-Allyl-p-mentha-6,8-dien-2-ols P1−P3 synthesized from carvone P are tested as corrosion inhibitors of steel in 1 M HCl using weight loss measurements, potentiodynamic polarisation and impedance spectroscopy (EIS methods. The addition of 2-allyl-p-mentha-6,8-dien-2-ols reduced the corrosion rate. Potentiodynamic polarisation studies clearly reveal that the presence of inhibitors does not change the mechanism of hydrogen evolution and that they act essentially as cathodic inhibitors. 2-Allyl-p-mentha-6,8-dien-2-ols tested adsorb on the steel surface according to Langmuir isotherm. From the adsorption isotherm some thermodynamic data for the adsorption process are calculated and discussed. EIS measurements show the increase of the charge-transfer resistance with the inhibitor concentration. The highest inhibition efficiency (92% is obtained for P1 at 3 g/L. The corrosion rate decreases with the rise of temperature. The corresponding activation energies are determined.

  1. Efeito anti-helmíntico do hidrolato de Mentha villosa Huds. (Lamiaceae em nematóides gastrintestinais de bovinos Anthelmintic effect of hidrolact of Mentha villosa Huds. (Lamiaceae in gastrointestinal nematodes of cattle

    Directory of Open Access Journals (Sweden)

    Érica Maria Nascimento

    2009-06-01

    Full Text Available Atualmente, o estudo da atividade anti-helmíntica de plantas medicinais em ruminantes tem atraído bastante interesse. Mentha villosa Huds. (Lamiaceae é uma das espécies de hortelã que tem sido utilizada popularmente devido às diversas propriedades medicinais, inclusive para o controle de verminoses. O presente estudo teve como objetivo testar a atividade anti-helmíntica do hidrolato dessa planta em bezerras infectadas por nematóides gastrintestinais, tanto in vitro, pelo método de coprocultura quantitativa, quanto in vivo, por meio do teste de redução no número de ovos de nematóides nas fezes dos hospedeiros. No teste in vitro, o hidrolato nas concentrações de 40%, 60% e 80% e 100% apresentou porcentagem de eficácia de 91,88%, 94,15%, 98,40% e 100%, respectivamente, mostrando atividade ovicida significativa sobre nematóides gastrintestinais em bezerras. Entretanto, os resultados do teste in vivo mostraram ausência de atividade anti-helmíntica do hidrolato de M. villosa na dose de 0,1ml kg dia-1, nos animais tratados.Currently, it has been of great interest to study the anthelmintic activity of medicinal plants in ruminants. Mentha villosa Huds. (Lamiaceae is one of the mint species that has been popularly used based on various medicinal properties, even for the control of nematode infections. This study aimed to test the anthelmintic activity of hidrolact of this plant, both in vitro, by the quantitative coproculture method, and in vivo, in calves infected with gastrointestinal nematodes, through the egg count reduction test in feces of the hosts. In in vitro tests, the hidrolact at the concentrations 40%, 60% and 80% and 100% obtained percentage of effectiveness of 91.88%, 94.15%, 98.40% and 100% respectively, showing significant ovicidal activity against gastrointestinal nematodes in calves. However, the hidrolact of M. villosa showed no in vivo anthelmintic activity at 0.1ml kg-1 day-1 on the treated animals.

  2. Protection against LPS-induced cartilage inflammation and degradation provided by a biological extract of Mentha spicata

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    Kott Laima S

    2010-05-01

    Full Text Available Abstract Background A variety of mint [Mentha spicata] has been bred which over-expresses Rosmarinic acid (RA by approximately 20-fold. RA has demonstrated significant anti-inflammatory activity in vitro and in small rodents; thus it was hypothesized that this plant would demonstrate significant anti-inflammatory activity in vitro. The objectives of this study were: a to develop an in vitro extraction procedure which mimics digestion and hepatic metabolism, b to compare anti-inflammatory properties of High-Rosmarinic-Acid Mentha spicata (HRAM with wild-type control M. spicata (CM, and c to quantify the relative contributions of RA and three of its hepatic metabolites [ferulic acid (FA, caffeic acid (CA, coumaric acid (CO] to anti-inflammatory activity of HRAM. Methods HRAM and CM were incubated in simulated gastric and intestinal fluid, liver microsomes (from male rat and NADPH. Concentrations of RA, CA, CO, and FA in simulated digest of HRAM (HRAMsim and CM (CMsim were determined (HPLC and compared with concentrations in aqueous extracts of HRAM and CM. Cartilage explants (porcine were cultured with LPS (0 or 3 μg/mL and test article [HRAMsim (0, 8, 40, 80, 240, or 400 μg/mL, or CMsim (0, 1, 5 or 10 mg/mL, or RA (0.640 μg/mL, or CA (0.384 μg/mL, or CO (0.057 μg/mL or FA (0.038 μg/mL] for 96 h. Media samples were analyzed for prostaglandin E2 (PGE2, interleukin 1β (IL-1, glycosaminoglycan (GAG, nitric oxide (NO and cell viability (differential live-dead cell staining. Results RA concentration of HRAMsim and CMsim was 49.3 and 0.4 μg/mL, respectively. CA, FA and CO were identified in HRAMsim but not in aqueous extract of HRAM. HRAMsim (≥ 8 μg/mL inhibited LPS-induced PGE2 and NO; HRAMsim (≥ 80 μg/mL inhibited LPS-induced GAG release. RA inhibited LPS-induced GAG release. No anti-inflammatory or chondroprotective effects of RA metabolites on cartilage explants were identified. Conclusions Our biological extraction procedure produces

  3. 薄荷精油抗菌活性研究%Antimicrobial Activities of Mentha haplocalyx Briq.Essential Oil

    Institute of Scientific and Technical Information of China (English)

    王微; 吴楠; 付玉杰; 祖元刚

    2007-01-01

    采用气相色谱-质谱联用仪分析了薄荷精油的成分,在鉴定出的21种成分中,异佛尔酮(41.22%)、p-石竹烯(10.01%)、斯巴醇(2.89%)、β-蒎烯(1.45%)和桉树脑(1.13%)为主要成分.采用琼脂扩散法和微量肉汤稀释法对薄荷精油的抗菌活性进行了研究.结果表明,枯草芽孢杆菌及变形杆菌出现最大的抑菌环.MIC实验中,薄荷精油的浓度范围为5.00%~0.039%.在所选的8种菌种中,变形杆菌的MIC及MBC值最低,分别为0.625%及1.25%.结果充分证明,薄荷精油对于所选的8种病原菌都有很好的抗菌活性,在食品及制药工业中有广阔的应用前景.%The sample of the Mentha haplocalyx Briq.essential oil was analyzed by GC-MS.From 21 compounds representing the oils,Isophorone(41.22%),β-Caryophyllene(10.01%),Spathulenol (2.89%),β-Pinene(1.45%)and Eucalyptole(1.13%)appeared as the main components.The screening of antimicrobial activity of the essential oil was evaluated using agar diffusion and broth microdilution methods.Tests showed that the oil presented the biggest inhibition zones in the two strains of Bacillus subtilis and Proteus vulgaris.The MICs of the oil against the tested microorganisms ranged from 5.00%to 0.039%.Of the 8 microorganisms,Proteus vulgaris presented the lowest MIC(0.625%)and MBC(1.25%)values.Results presented here may suggest that the essential oil of Mentha haplocalyx Briq.possesses antimicrobial properties,and therefore is a potential source of antimicrobial ingredientsfor food and pharmaceutical industry.

  4. Chemical Composition and In Vitro Antibacterial Activity of Mentha spicata Essential Oil against Common Food-Borne Pathogenic Bacteria.

    Science.gov (United States)

    Shahbazi, Yasser

    2015-01-01

    The aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of Mentha spicata plant against common food-borne pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli O157:H7). Chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (GC-MS). The antibacterial activity of the essential oil was evaluated by broth microdilution method and agar disk diffusion assay. According to the result of GC-MS analysis, 18 components were identified, accounting for 99.89% of the whole essential oil. The main components were carvone (78.76%), limonene (11.50%), β-bourbonene (11.23%), cis-dihydrocarveol (1.43%), trans-caryophyllene (1.04%), menthone (1.01%), menthol (1%), and terpinen-4-ol (0.99). The essential oil exhibited moderate level of antibacterial activity against all test microorganisms. In general, Gram-positive bacteria were more susceptible to M. spicata essential oil than Gram-negative bacteria. L. monocytogenes was the most sensitive of the microorganisms to the antibacterial activity of M. spicata essential oil (inhibition zone = 22 mm and MIC and MBC = 2.5 µL/mL). Based on our results, the essential oil of M. spicata plant collected from Kermanshah province, west of Iran, has a potential to be applied as antibacterial agent. PMID:26351584

  5. Mentha spicata Essential Oil: Chemical Composition, Antioxidant and Antibacterial Activities against Planktonic and Biofilm Cultures of Vibrio spp. Strains

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    Mejdi Snoussi

    2015-08-01

    Full Text Available Chemical composition, antioxidant and anti-Vibrio spp. activities of the essential oil isolated from the aerial parts of Mentha spicata L. (spearmint are investigated in the present study. The effect of the essential oil on Vibrio spp. biofilm inhibition and eradication was tested using the XTT assay. A total of 63 chemical constituents were identified in spearmint oil using GC/MS, constituting 99.9% of the total identified compounds. The main components were carvone (40.8% ± 1.23% and limonene (20.8% ± 1.12%. The antimicrobial activity against 30 Vibrio spp. strains (16 species was evaluated by disc diffusion and microdilution assays. All microorganisms were strongly affected, indicating an appreciable antimicrobial potential of the oil. Moreover, the investigated oil exhibited high antioxidant potency, as assessed by four different tests in comparison with BHT. The ability of the oil, belonging to the carvone chemotype, to inhibit or reduce Vibrio spp. biofilm warrants further investigation to explore the use of natural products in antibiofilm adhesion and reinforce the possibility of its use in the pharmaceutical or food industry as a natural antibiotic and seafood preservative against Vibrio contamination.

  6. Tipos de estacas e substratos na propagação vegetativa da menta (Mentha arvensis L.

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    H.T.R Amaro

    2013-01-01

    Full Text Available O trabalho teve como objetivo avaliar a propagação vegetativa da menta utilizando diferentes tipos de estacas e substratos. O experimento foi conduzido no Horto de Plantas Medicinais da Unimontes, campus Janaúba - MG. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 2 x 4 (dois tipos de estacas e quatro diferentes substratos com quatro repetições, sendo cada parcela representada por seis estacas. Foram analisadas as variáveis comprimento de parte aérea e de raízes, massa seca de parte aérea e de raízes e número total de brotações formadas por planta. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Scott-Knott a 5% de probabilidade. A interação entre os fatores estacas e substratos não foi significativa para as variáveis estudadas, passando-se a estudar o efeito isolado de cada fator. A propagação de Mentha arvensis L. pode ser realizada tanto por estacas apicais como medianas, utilizando o substrato solo + areia + esterco bovino (2:1:1 para a produção de mudas de qualidade.

  7. Nutritional and sensory quality during refrigerated storage of fresh-cut mints (Mentha×piperita and M. spicata).

    Science.gov (United States)

    Curutchet, Ana; Dellacassa, Eduardo; Ringuelet, Jorge A; Chaves, Alicia R; Viña, Sonia Z

    2014-01-15

    The effect of storage time on quality attributes of refrigerated fresh-cut mints (Mentha×piperita and M. spicata) was studied. Atmosphere composition, respiratory activity, weight loss, surface colour, total chlorophyll, carotenoids, browning potential, total phenols, flavonoids, radical-scavenging activity, ascorbic acid and essential oil yield and composition were analysed. Respiratory activity of peppermint and spearmint samples diminished moderately (42% and 28%, respectively) after 21days at 0°C. A slight modification of the internal atmosphere was achieved. Surface colour, chlorophyll, carotenoid and antioxidant compounds remained almost constant. The yield of essential oil did not change or it showed an apparent increase after 21days at 0°C, depending on plant growth stage. The characteristic flavour components of peppermint (menthone and menthol) increased, while the contents of the main constituents of spearmint essential oil showed minor variations after storage. The conditions assayed for packaging and storing fresh-cut mints were adequate to achieve a relatively long shelf life and they retained their antioxidant properties. PMID:24054235

  8. Investigation of the effects of peppermint (Mentha piperita on the biochemical and anthropometric profile of university students

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    Sandra Maria Barbalho

    2011-09-01

    Full Text Available The hypolipidemic effects of several medicinal plants have already been demonstrated, but many plants commonly used to treat diseases still need to be studied. Peppermint (Mentha piperita is widely consumed by the population for different purposes, but not for the treatment of dyslipidemias. The objective of this study was to examine the effects of this plant on human biochemical and anthropometric profiles and blood pressure, based on the administration of peppermint juice twice daily for 30 days. Blood samples were collected before and after the treatment in order to determine the glycemic and lipid profiles, and the Body Mass Index (BMI analysis was performed. Results indicated that 41.5% of the subjects showed a reduction in glycemia, 66.9% in total cholesterol levels, 58.5% in triacylglycerides, 52.3% in LDL-c (low-density lipoproteins indices, 70% in GOT (glutamic-oxaloacetic transaminase levels, 74.5% in GPT (glutamic-pyruvic transaminase levels, and that 52% presented an increase in HDL-c (high-density lipoprotein cholesterol indices. Also, 52.5% showed a decrease in blood pressure and 48.7% in BMI. The use of peppermint by humans can be considered beneficial in the prevention and treatment of risk factors of chronic degenerative diseases.

  9. Plant growth-promoting effects of native Pseudomonas strains on Mentha piperita (peppermint): an in vitro study.

    Science.gov (United States)

    Santoro, M V; Cappellari, L R; Giordano, W; Banchio, E

    2015-11-01

    Plant growth-promoting rhizobacteria (PGPR) affect growth of host plants through various direct and indirect mechanisms. Three native PGPR (Pseudomonas putida) strains isolated from rhizospheric soil of a Mentha piperita (peppermint) crop field near Córdoba, Argentina, were characterised and screened in vitro for plant growth-promoting characteristics, such as indole-3-acetic acid (IAA) production, phosphate solubilisation and siderophore production, effects of direct inoculation on plant growth parameters (shoot fresh weight, root dry weight, leaf number, node number) and accumulation and composition of essential oils. Each of the three native strains was capable of phosphate solubilisation and IAA production. Only strain SJ04 produced siderophores. Plants directly inoculated with the native PGPR strains showed increased shoot fresh weight, glandular trichome number, ramification number and root dry weight in comparison with controls. The inoculated plants had increased essential oil yield (without alteration of essential oil composition) and biosynthesis of major essential oil components. Native strains of P. putida and other PGPR have clear potential as bio-inoculants for improving productivity of aromatic crop plants. There have been no comparative studies on the role of inoculation with native strains on plant growth and secondary metabolite production (specially monoterpenes). Native bacterial isolates are generally preferable for inoculation of crop plants because they are already adapted to the environment and have a competitive advantage over non-native strains.

  10. Extraction, Preliminary Characterization and Evaluation of in Vitro Antitumor and Antioxidant Activities of Polysaccharides from Mentha piperita

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    Xin Liu

    2014-09-01

    Full Text Available This study describes the extraction, preliminary characterization and evaluation of the in vitro antitumor and antioxidant activities of polysaccharides extracted from Mentha piperita (MPP. The optimal parameters for the extraction of MPP were obtained by Box-Behnken experimental design and response surface methodology (RSM at the ratio of water to raw material of 20, extraction time of 1.5 h and extraction temperature at 80 °C. Chemical composition analysis showed that MPP was mainly composed of glucuronic acid, galacturonic acid, glucose, galactose and arabinose, and the molecular weight of its two major fractions were estimated to be about 2.843 and 1.139 kDa, respectively. In vitro bioactivity experiments showed that MPP not only inhibited the growth of A549 cells but possessed potent inhibitory action against DNA topoisomerase I (topo I, and an appreciative antioxidant action as well. These results indicate that MPP may be useful for developing safe natural health products.

  11. Impact assessment of mercury accumulation and biochemical and molecular response of Mentha arvensis: a potential hyperaccumulator plant.

    Science.gov (United States)

    Manikandan, R; Sahi, S V; Venkatachalam, P

    2015-01-01

    The present study was focused on examining the effect of Hg oxidative stress induced physiochemical and genetic changes in M. arvensis seedlings. The growth rate of Hg treated seedlings was decreased to 56.1% and 41.5% in roots and shoots, respectively, compared to the control. Accumulation of Hg level in both roots and shoots was increased with increasing the concentration of Hg. Superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities were found to be increased with increasing the Hg concentration up to 20 mg/L; however, it was decreased at 25 mg/L Hg concentration. The POX enzyme activity was positively correlated with Hg dose. The changes occurring in the random amplification of ploymorphic DNA (RAPD) profiles generated from Hg treated seedlings included variations in band intensity, disappearance of bands, and appearance of new bands compared with the control seedlings. It was concluded that DNA polymorphisms observed with RAPD profile could be used as molecular marker for the evaluation of heavy metal induced genotoxic effects in plant species. The present results strongly suggested that Mentha arvensis could be used as a potential phytoremediator plant in mercury polluted environment.

  12. Effect of hydrocooling, packaging, and cold storage on the post-harvest quality of peppermint (Mentha piperita L.

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    C.K.R. BARBOSA

    2016-01-01

    Full Text Available ABSTRACT The purpose of this study was to assess the effects of hydrocooling and polyethylene terephthalate (PET perforated and non-perforated packaging on post-harvest quality of peppermint (Mentha piperita L. leaves stored at 5 and 25ºC. The experiments were conducted using a subdivided parcel layout in a completely randomized design with four replicates. Each parcel used a 2x3 layout (two hydrocooling treatments and three packaging treatments with subparcels for storage time. The shelf life, chlorophyll content, mass loss (ML, relative water content (RWC, total soluble sugars (TSS, reducing sugars (Red, non-reducing sugars (NRed and starch of the leaves were determined. Cold storage increased the shelf life of the peppermint by up to 64 times (>15 days. Hydrocooling increased the RWC of the leaves. Plastic packaging was effective in maintaining the RWC. Unperforated packages more effectively prevented ML in the peppermint leaves and prevented the leaves from wilting for a longer period of time. The chlorophyll content of the leaves varied according to the treatment. The soluble sugars and starch levels varied according to RWC. Hydrocooling with the use of non-perforated plastic packages was the most effective method for maintaining the postharvest quality of the peppermint.

  13. Comparative study on the antioxidant effect of aqueous and ethanolic extracts of Mentha pulegium L. grown at two different locations

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    BRAHMI F

    2014-11-01

    Full Text Available Aqueous and ethanolic extracts of Mentha pulegium L. (Lamiaceae from two different locations Tizi-Ouzou and Bejaia in Algeria were examined in vitro. Phenolic compounds and antioxidant activity were analyzed. Measurement of total phenolic and total flavonoids contents of the extracts of M. pulegium were achieved by using Folin-Ciocalteu and chloride aluminium methods, respectively. The total phenolic content of water extract from Tizi-Ouzou location was found significantly higher (55.78  2.78 mg GAE/g DW. The flavonoids content was also higher in the extracts from this location for both ethanolic and water extracts (2.17  0.12; 2.04  0.03 mg QE/g DW, respectively. Antioxidant activities were assessed by five in vitro antioxidant assays. Results showed that the two M. pulegium provenances were significantly different according to their antioxidant activity. Indeed, aqueous extract issued from Bejaia plant exhibited stronger antioxidant activity. For instance, Bejaia provenance shoots showed lower IC50 value of 20.35 μg/ mL for H2O2 test.

  14. Mentha spicata Essential Oil: Chemical Composition, Antioxidant and Antibacterial Activities against Planktonic and Biofilm Cultures of Vibrio spp. Strains.

    Science.gov (United States)

    Snoussi, Mejdi; Noumi, Emira; Trabelsi, Najla; Flamini, Guido; Papetti, Adele; De Feo, Vincenzo

    2015-01-01

    Chemical composition, antioxidant and anti-Vibrio spp. activities of the essential oil isolated from the aerial parts of Mentha spicata L. (spearmint) are investigated in the present study. The effect of the essential oil on Vibrio spp. biofilm inhibition and eradication was tested using the XTT assay. A total of 63 chemical constituents were identified in spearmint oil using GC/MS, constituting 99.9% of the total identified compounds. The main components were carvone (40.8% ± 1.23%) and limonene (20.8% ± 1.12%). The antimicrobial activity against 30 Vibrio spp. strains (16 species) was evaluated by disc diffusion and microdilution assays. All microorganisms were strongly affected, indicating an appreciable antimicrobial potential of the oil. Moreover, the investigated oil exhibited high antioxidant potency, as assessed by four different tests in comparison with BHT. The ability of the oil, belonging to the carvone chemotype, to inhibit or reduce Vibrio spp. biofilm warrants further investigation to explore the use of natural products in antibiofilm adhesion and reinforce the possibility of its use in the pharmaceutical or food industry as a natural antibiotic and seafood preservative against Vibrio contamination.

  15. Phenolic content, antioxidant and astroprotective response to oxidative stress of ethanolic extracts of Mentha longifolia from Sinai.

    Science.gov (United States)

    Eissa, Tarek F; González-Burgos, Elena; Carretero, M Emilia; Gómez-Serranillos, M Pilar

    2014-10-01

    The aerial parts ofMentha longifolia L. are used as herbal remedies for curing different diseases through traditional Bedouin medicine. The antioxidant activity of the ethanolic extracts of M longifolia was investigated measuring peroxyl radical-scavenging activity by ORAC assay, with Trolox (a water-soluble analogue of α-tocopherol) employed as reference compound. In addition, the total content of phenolic compounds estimated by the Folin-Ciocalteau method and the identification of the polyphenols using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) have been performed. Furthermore, the effect of these extracts on cell viability and intracellular ROS production was assayed using the U373-MG human astrocytoma cell line in a H2O2-induced oxidative stress model. Results showed that the major type of polyphenols found were benzoic acids, cinnamic acids, flavones and flavanones. The total phenolic content was 37.7 mg gallic acid/g sample and the ORAC value was 1.355 .mol TE/mg sample. The data obtained in cellular assays demonstrated that these ethanolic extracts protected H2O2-induced astrocyte damage by increasing cell viability and inhibiting production of intracellular ROS. These results suggest that the investigated extracts obtained from the aerial parts of M longifolia have antioxidant potential related to their phenol content which have important beneficial health effects, especially in those disease associated with ROS.

  16. Metabolite profile, antioxidant capacity, and inhibition of digestive enzymes in infusions of peppermint (Mentha piperita) grown under drought stress.

    Science.gov (United States)

    Figueroa-Pérez, Marely G; Rocha-Guzmán, Nuria Elizabeth; Pérez-Ramírez, Iza F; Mercado-Silva, Edmundo; Reynoso-Camacho, Rosalía

    2014-12-10

    Peppermint (Mentha piperita) infusions represent an important source of antioxidants, which can be enhanced by inducing abiotic stress in plants. The aim of this study was to evaluate the effect of drought stress on peppermint cultivation as well as the metabolite profile, antioxidant capacity, and inhibition of digestive enzymes of resulting infusions. At 45 days after planting, irrigation was suppressed until 85 (control), 65, 35, 24, and 12% soil moisture (SM) was reached. The results showed that 35, 24, and 12% SM decreased fresh (20%) and dry (5%) weight. The 35 and 24% SM treatments significantly increased total phenolic and flavonoid contents as well as antioxidant capacity. Coumaric acid, quercetin, luteolin, and naringenin were detected only in some drought treatments; however, in these infusions, fewer amino acids and unsaturated fatty acids were identified. The 24 and 12% SM treatments slightly improved inhibition of pancreatic lipase and α-amylase activity. Therefore, induction of moderate water stress in peppermint is recommended to enhance its biological properties.

  17. In Vitro Susceptibility of Pythium insidiosum to Melaleuca alternifolia, Mentha piperita and Origanum vulgare Essential Oils Combinations.

    Science.gov (United States)

    de Souza Silveira Valente, Júlia; de Oliveira da Silva Fonseca, Anelise; Denardi, Laura Bedin; Dal Ben, Vanessa Silveira; de Souza Maia Filho, Fernando; Baptista, Cristiane Telles; Braga, Caroline Quintana; Zambrano, Cristina Gomes; Alves, Sydney Hartz; de Avila Botton, Sônia; Pereira, Daniela Isabel Brayer

    2016-08-01

    Pythium insidiosum is the etiologic agent of pythiosis, a severe and emerging disease that affects mammals. Failure of conventional antifungal therapies is partially justified by the absence of ergosterol in the plasma membrane of this oomycete. Despite research advancement, the treatment of pythiosis has not been not fully established. The present study investigated the in vitro susceptibility profile of Brazilian isolates of P. insidiosum (n = 20) against Melaleuca alternifolia, Mentha piperita and Origanum vulgare essential oils, and their combinations. Susceptibility tests were performed according to CLSI M38-A2 protocol, and combinations were evaluated by the microdilution cherkerboard method. All tested essential oils showed antimicrobial activity against P. insidiosum, and the greatest activity of O. vulgare was highlighted. Synergistic and/or indifferent effect was observed for all combinations evaluated, especially the M. piperita and O. vulgare combination, which showed 65 % synergism. This is the first study to report in vitro combinations of essential oils against P. insidiosum indicating the susceptibility of this oomycete to M. alternifolia, M. piperita and O. vulgare essential oils, as well as their combinations. PMID:27209011

  18. In vitro induction of α-pinene, pulegone, menthol, menthone and limonene in cell suspension culture of pennyroyal (Mentha pulegium).

    Science.gov (United States)

    Darvishi, E; Kahrizi, D; Bahraminejad, S; Mansouri, M

    2016-01-01

    Medicinal plants are known as important sources of secondary metabolites. Because of the economic value of pennyroyal [Mentha pulegium L. (Lamiaceae)] in food industries, propagation of this valuable plant has special importance. Plant cell suspension culture can increase some produced components. The aim of this research was performing cell culture for induction of some secondary metabolites of M. pulegium and compares it with native one. The MS medium was used for suspension culture. To investigate quantitative materials, 4 levels of yeast extract elicitor (20, 40, 60 and 80 mg/L) and salicylic acid in 4 levels (2, 4, 6 and 8 mg/L) were used. Obtained extracts were analyzed by GC-MS. Statistical analysis showed that the amount of limonene, menthone, menthol and α-pinene were more than mentioned compounds in natural plant as control. The maximum amount of this metabolites were obtained as limonene (in 60 mg/l yeast extract), menthone (in 40 mg/l yeast extract and 2 mg/l salicylic acid), menthol (in 6 mg/l salicylic acid) and α-pinene (in 4 mg/l salicylic acid) in the M. pulegium cell culture. The Pulegone was fond more in natural plants than cell culture mass. The most important secondary metabolites were increased by cell culture containing of salicylic acid and yeast extract elicitors in M. pulegume. PMID:27064866

  19. Mentha spicata Essential Oil: Chemical Composition, Antioxidant and Antibacterial Activities against Planktonic and Biofilm Cultures of Vibrio spp. Strains.

    Science.gov (United States)

    Snoussi, Mejdi; Noumi, Emira; Trabelsi, Najla; Flamini, Guido; Papetti, Adele; De Feo, Vincenzo

    2015-01-01

    Chemical composition, antioxidant and anti-Vibrio spp. activities of the essential oil isolated from the aerial parts of Mentha spicata L. (spearmint) are investigated in the present study. The effect of the essential oil on Vibrio spp. biofilm inhibition and eradication was tested using the XTT assay. A total of 63 chemical constituents were identified in spearmint oil using GC/MS, constituting 99.9% of the total identified compounds. The main components were carvone (40.8% ± 1.23%) and limonene (20.8% ± 1.12%). The antimicrobial activity against 30 Vibrio spp. strains (16 species) was evaluated by disc diffusion and microdilution assays. All microorganisms were strongly affected, indicating an appreciable antimicrobial potential of the oil. Moreover, the investigated oil exhibited high antioxidant potency, as assessed by four different tests in comparison with BHT. The ability of the oil, belonging to the carvone chemotype, to inhibit or reduce Vibrio spp. biofilm warrants further investigation to explore the use of natural products in antibiofilm adhesion and reinforce the possibility of its use in the pharmaceutical or food industry as a natural antibiotic and seafood preservative against Vibrio contamination. PMID:26262604

  20. Nutritional and sensory quality during refrigerated storage of fresh-cut mints (Mentha×piperita and M. spicata).

    Science.gov (United States)

    Curutchet, Ana; Dellacassa, Eduardo; Ringuelet, Jorge A; Chaves, Alicia R; Viña, Sonia Z

    2014-01-15

    The effect of storage time on quality attributes of refrigerated fresh-cut mints (Mentha×piperita and M. spicata) was studied. Atmosphere composition, respiratory activity, weight loss, surface colour, total chlorophyll, carotenoids, browning potential, total phenols, flavonoids, radical-scavenging activity, ascorbic acid and essential oil yield and composition were analysed. Respiratory activity of peppermint and spearmint samples diminished moderately (42% and 28%, respectively) after 21days at 0°C. A slight modification of the internal atmosphere was achieved. Surface colour, chlorophyll, carotenoid and antioxidant compounds remained almost constant. The yield of essential oil did not change or it showed an apparent increase after 21days at 0°C, depending on plant growth stage. The characteristic flavour components of peppermint (menthone and menthol) increased, while the contents of the main constituents of spearmint essential oil showed minor variations after storage. The conditions assayed for packaging and storing fresh-cut mints were adequate to achieve a relatively long shelf life and they retained their antioxidant properties.

  1. Anthelmintic activity of Cymbopogon martinii, Cymbopogon schoenanthus and Mentha piperita essential oils evaluated in four different in vitro tests.

    Science.gov (United States)

    Katiki, L M; Chagas, A C S; Bizzo, H R; Ferreira, J F S; Amarante, A F T

    2011-12-29

    Anthelmintic resistance is a worldwide concern in small ruminant industry and new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Mentha piperita, Cymbopogon martinii and Cymbopogon schoenanthus essential oils were evaluated against developmental stages of trichostrongylids from sheep naturally infected (95% Haemonchus contortus and 5% Trichostrogylus spp.) through the egg hatch assay (EHA), larval development assay (LDA), larval feeding inhibition assay (LFIA), and the larval exsheathment assay (LEA). The major constituent of the essential oils, quantified by gas chromatography for M. piperita oil was menthol (42.5%), while for C. martinii and C. schoenanthus the main component was geraniol (81.4% and 62.5%, respectively). In all in vitro tests C. schoenanthus essential oil had the best activity against ovine trichostrongylids followed by C. martini, while M. piperita presented the least activity. Cymbopogon schoenanthus essential oil had LC(50) value of 0.045 mg/ml in EHA, 0.063 mg/ml in LDA, 0.009 mg/ml in LFIA, and 24.66 mg/ml in LEA. The anthelmintic activity of essential oils followed the same pattern in all in vitro tests, suggesting C. schoenanthus essential oil could be an interesting candidate for nematode control, although in vivo studies are necessary to validate the anthelmintic properties of this oil.

  2. ULTRASTRUCTURAL CHANGES IN Schistosoma mansoni MALE WORMS AFTER in vitro INCUBATION WITH THE ESSENTIAL OIL OF Mentha x villosa Huds

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    Thiago José MATOS-ROCHA

    2016-01-01

    Full Text Available Introduction: The essential oil Mentha x villosa (MVEO has a wide range of actions, including antibacterial, antifungal, antiprotozoal and schistosomicidal actions. The present study aimed to investigate the ultrastructural changes of MVEO on the tegument of adult Schistosoma mansoni. Materials and Methods: Different concentrations of MVEO were tested on S. mansoni adult worms in vitro. Ultrastructural changes on the tegument of these adult worms were evaluated using scanning electron microscopy (SEM and transmission electron microscopy (TEM. Results: The MVEO caused the death of all worms at 500 μg mL-1 after 24 h. After 24h of 500 μg mL-1 MVEO treatment, bubble lesions were observed over the entire body of worms and they presented loss of tubercles in some regions of the ventral portion. In the evaluation by TEM, S. mansoni adult worms treated with MVEO, 500 μg mL-1, presented changes in the tegument and vacuoles in the syncytial matrix region. Glycogen granules close to the muscle fibers were visible. Conclusion: The ability of MVEO to cause extensive ultrastructural damage to S. mansoni adult worms correlates with its schistosomicidal effects and confirms earlier findings with S. mansoni.

  3. Chemical Composition and In Vitro Antibacterial Activity of Mentha spicata Essential Oil against Common Food-Borne Pathogenic Bacteria

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    Yasser Shahbazi

    2015-01-01

    Full Text Available The aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of Mentha spicata plant against common food-borne pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli O157:H7. Chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (GC-MS. The antibacterial activity of the essential oil was evaluated by broth microdilution method and agar disk diffusion assay. According to the result of GC-MS analysis, 18 components were identified, accounting for 99.89% of the whole essential oil. The main components were carvone (78.76%, limonene (11.50%, β-bourbonene (11.23%, cis-dihydrocarveol (1.43%, trans-caryophyllene (1.04%, menthone (1.01%, menthol (1%, and terpinen-4-ol (0.99. The essential oil exhibited moderate level of antibacterial activity against all test microorganisms. In general, Gram-positive bacteria were more susceptible to M. spicata essential oil than Gram-negative bacteria. L. monocytogenes was the most sensitive of the microorganisms to the antibacterial activity of M. spicata essential oil (inhibition zone = 22 mm and MIC and MBC = 2.5 µL/mL. Based on our results, the essential oil of M. spicata plant collected from Kermanshah province, west of Iran, has a potential to be applied as antibacterial agent.

  4. Antimicrobial and Antioxidant Activity of the Essential Oils Obtained from Mentha longifolia L. Hudson, Dryed by Three Different Techniques

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    Dragana Stanisavljević, , , , and

    2013-11-01

    Full Text Available The way of drying the fresh herbal material influences the chemical content and the biological activities of their essential oils. The influence of the different drying methods of the herb Mentha longifolia (L. Hudson on the antioxidant and antimicrobial activity of the extracted essential oils has been analyzed in this study. Drying has been carried out in three ways: in the natural way, in the laboratory oven (45°C and in the absorptional low-temperature condensational drier (35°C. The antioxidant activity of the essential oil has been estimated by FRAP and DPPH assays, while the antimicrobial activity has been estimated by the diffusible and micro-delusional method, testing on the nine types of bacteria and two types of fungi. The essential oil obtained from the herb dried in the natural way has shown the highest antioxidant activity and the lowest from the herb dried in the laboratory oven. Bacillus subtilis , Micrococcus luteus and Enterococcus faecalis have shown the highest sensitivity on the three samples. The oil obtained from the herb dried in the absorptional low-temperature drier has shown the strongest antimicrobial effect.

  5. Chemical Composition and In Vitro Antibacterial Activity of Mentha spicata Essential Oil against Common Food-Borne Pathogenic Bacteria.

    Science.gov (United States)

    Shahbazi, Yasser

    2015-01-01

    The aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of Mentha spicata plant against common food-borne pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli O157:H7). Chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (GC-MS). The antibacterial activity of the essential oil was evaluated by broth microdilution method and agar disk diffusion assay. According to the result of GC-MS analysis, 18 components were identified, accounting for 99.89% of the whole essential oil. The main components were carvone (78.76%), limonene (11.50%), β-bourbonene (11.23%), cis-dihydrocarveol (1.43%), trans-caryophyllene (1.04%), menthone (1.01%), menthol (1%), and terpinen-4-ol (0.99). The essential oil exhibited moderate level of antibacterial activity against all test microorganisms. In general, Gram-positive bacteria were more susceptible to M. spicata essential oil than Gram-negative bacteria. L. monocytogenes was the most sensitive of the microorganisms to the antibacterial activity of M. spicata essential oil (inhibition zone = 22 mm and MIC and MBC = 2.5 µL/mL). Based on our results, the essential oil of M. spicata plant collected from Kermanshah province, west of Iran, has a potential to be applied as antibacterial agent.

  6. Volatile compounds and sensory analysis of both harvests of double-cut Yakima peppermint (Mentha piperita L.).

    Science.gov (United States)

    Chen, Michael Z; Trinnaman, Laurence; Bardsley, Kathryn; St Hilaire, Craig J; Da Costa, Neil C

    2011-09-01

    North American peppermint oils are widely regarded as some of the most superior peppermint oils commercially available. Amongst them is Yakima double-cut peppermint oil (Mentha piperita L.). It has an aroma described as strong, refreshing, minty, slightly creamy, and very herbaceous with some of the most desirable flavor notes among peppermint oils. The peppermint is grown in the Yakima Valley of Washington State, USA. As one of the most northerly grown peppermints with longer days, it is unique as there are 2 harvests of the mint leaves in one season. For this study, samples of fresh and dried (hay) leaves were collected from both harvests; 1st cut, July and 2nd cut, September. Steam-distilled oils were produced from each harvest and analyzed by gas chromatography-mass spectrometry to give detailed profiles. The aroma and taste sensory attributes of each oil were evaluated. In addition, menthyl formate has previously been reported only once in the literature, but not as definitively as required by current flavor-regulatory bodies for use as a natural flavoring ingredient. It was conclusively reported in these peppermint oils.

  7. Analysis of the efficacy of the peppermint oil (Mentha piperita L. on irritable bowel syndrome: an evidence-based review

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    Joana Costa Gomes

    2013-04-01

    Full Text Available Objective: To evaluate the efficacy of peppermint oil (Mentha piperita L. on individuals diagnosed with irritable bowel syndrome (IBS regarding symptom improvement and quality of life enhancement. Methods: Literature search was conducted according to evidence based on methodology review at Trip database, National Guideline Clearinghouse, Guidelines finder, Cochrane Library, Dare, Bandolier and Medline, as well as using the MeSH index terms “Irritable bowel syndrome” and “peppermint oil”. We selected practice guidelines, systematic reviews, meta-analysis and randomized controlled trials that evaluated peppermint oil (PO efficacy in improving symptoms and/or quality of life when compared to placebo or other approved therapy for IBS. Results and Discussion: A total of eight articles were selected including five practice guidelines and three systematic reviews/ meta-analysis. Existing evidence suggests that there are enough data to support the use of peppermint oil in IBS for overall symptomatic relief, especially when abdominal pain is the dominant symptom, when compared to placebo (with an odds ratio of 2.7; CI 95%, 1.6 to 4.8 and NNT of 3. There is also evidence regarding overall quality of life improvement when using PO compared to placebo (p<0.001. Conclusion: So far, evidence suggests that PO should be considered for IBS patients, especially in the presence of abdominal pain (Strength of recommendation B. However, more high methodological quality studies that evaluate long-term efficacy and security of PO are needed.

  8. Plant growth-promoting effects of native Pseudomonas strains on Mentha piperita (peppermint): an in vitro study.

    Science.gov (United States)

    Santoro, M V; Cappellari, L R; Giordano, W; Banchio, E

    2015-11-01

    Plant growth-promoting rhizobacteria (PGPR) affect growth of host plants through various direct and indirect mechanisms. Three native PGPR (Pseudomonas putida) strains isolated from rhizospheric soil of a Mentha piperita (peppermint) crop field near Córdoba, Argentina, were characterised and screened in vitro for plant growth-promoting characteristics, such as indole-3-acetic acid (IAA) production, phosphate solubilisation and siderophore production, effects of direct inoculation on plant growth parameters (shoot fresh weight, root dry weight, leaf number, node number) and accumulation and composition of essential oils. Each of the three native strains was capable of phosphate solubilisation and IAA production. Only strain SJ04 produced siderophores. Plants directly inoculated with the native PGPR strains showed increased shoot fresh weight, glandular trichome number, ramification number and root dry weight in comparison with controls. The inoculated plants had increased essential oil yield (without alteration of essential oil composition) and biosynthesis of major essential oil components. Native strains of P. putida and other PGPR have clear potential as bio-inoculants for improving productivity of aromatic crop plants. There have been no comparative studies on the role of inoculation with native strains on plant growth and secondary metabolite production (specially monoterpenes). Native bacterial isolates are generally preferable for inoculation of crop plants because they are already adapted to the environment and have a competitive advantage over non-native strains. PMID:26012535

  9. Impact assessment of mercury accumulation and biochemical and molecular response of Mentha arvensis: a potential hyperaccumulator plant.

    Science.gov (United States)

    Manikandan, R; Sahi, S V; Venkatachalam, P

    2015-01-01

    The present study was focused on examining the effect of Hg oxidative stress induced physiochemical and genetic changes in M. arvensis seedlings. The growth rate of Hg treated seedlings was decreased to 56.1% and 41.5% in roots and shoots, respectively, compared to the control. Accumulation of Hg level in both roots and shoots was increased with increasing the concentration of Hg. Superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities were found to be increased with increasing the Hg concentration up to 20 mg/L; however, it was decreased at 25 mg/L Hg concentration. The POX enzyme activity was positively correlated with Hg dose. The changes occurring in the random amplification of ploymorphic DNA (RAPD) profiles generated from Hg treated seedlings included variations in band intensity, disappearance of bands, and appearance of new bands compared with the control seedlings. It was concluded that DNA polymorphisms observed with RAPD profile could be used as molecular marker for the evaluation of heavy metal induced genotoxic effects in plant species. The present results strongly suggested that Mentha arvensis could be used as a potential phytoremediator plant in mercury polluted environment. PMID:25654134

  10. Impact Assessment of Mercury Accumulation and Biochemical and Molecular Response of Mentha arvensis: A Potential Hyperaccumulator Plant

    Directory of Open Access Journals (Sweden)

    R. Manikandan

    2015-01-01

    Full Text Available The present study was focused on examining the effect of Hg oxidative stress induced physiochemical and genetic changes in M. arvensis seedlings. The growth rate of Hg treated seedlings was decreased to 56.1% and 41.5% in roots and shoots, respectively, compared to the control. Accumulation of Hg level in both roots and shoots was increased with increasing the concentration of Hg. Superoxide dismutase (SOD, catalase (CAT, and ascorbate peroxidase (APX activities were found to be increased with increasing the Hg concentration up to 20 mg/L; however, it was decreased at 25 mg/L Hg concentration. The POX enzyme activity was positively correlated with Hg dose. The changes occurring in the random amplification of ploymorphic DNA (RAPD profiles generated from Hg treated seedlings included variations in band intensity, disappearance of bands, and appearance of new bands compared with the control seedlings. It was concluded that DNA polymorphisms observed with RAPD profile could be used as molecular marker for the evaluation of heavy metal induced genotoxic effects in plant species. The present results strongly suggested that Mentha arvensis could be used as a potential phytoremediator plant in mercury polluted environment.

  11. In vitro study on the antimicrobial effect of hydroalcoholic extracts from Mentha arvensis L. (Lamiaceae) against oral pathogens=Estudo in vitro do efeito antimicrobiano dos extratos hidroalcólicos de Mentha arvensis L. (Lamiaceae) contra patógenos orais

    OpenAIRE

    Rogério Antonio Freitag; Gladis Aver Ribeiro; Patrícia da Silva Nascente; Rosana Serpa; Rafael Guerra Lund; Francisco Augusto Burkert Del Pino

    2012-01-01

    In vitro tests could be a valuable tool for the evaluation of medicinal plants’ antimicrobial activity. Mentha arvensis of the Lamiaceae family is one of the most frequently traditional plants used in Brazil. Hydroalcoholic extracts of M. arvensis were analyzed for antimicrobial activity on Streptococcus mutans, Streptococcus sobrinus and Candida albicans. Three different assays (agar diffusion, broth macro- and micro-dilution methods) were used to evaluate antimicrobial activity. Although hy...

  12. UN MÉTODO PARA LA DESINFECCIÓN Y EL ESTABLECIMIENTO In Vitro DE LA MENTA JAPONESA (Mentha arvensis L.

    Directory of Open Access Journals (Sweden)

    E. Héctor

    2005-01-01

    Full Text Available El género Mentha constituye una importante fuente de materia prima para varias industrias como la perfumería, cosmética, licorería y farmacéutica. En el estado de Chihuahua, México, se han introducido en los últimos años varias especies de este género con propósitos comerciales, y se ha empleado con relativo éxito la propagación in vitro en la multiplicación masiva de una de ellas. La ocurrencia de contaminaciones endógenas ha sido un problema para el establecimiento in vitro de esta y otras especies comerciales. En este trabajo se presenta un método para la desinfección y el establecimiento in vitro de explantes de Mentha arvensis L., después de una previa detección de dos géneros de bacterias (Streptobacillus y Enterobacter presentes en abundancia en los explantes de esta especie. El método se basa en la combinación del empleo de cloranfenicol en un tratamiento previo a la implantación y como constituyente del medio de cultivo, y permitió la obtención de un 65 % de explantes libres de microorganismos. Se discuten las causas de la presencia de contaminación en los explantes de M. arvensis L. y se recomienda el método para su aplicación en otras especies de Mentha.

  13. Antimicrobial properties of plant extracts of Thymus vulgaris L., Ziziphora tenuior L. and Mentha Spicata L., against important foodborne pathogens in vitro

    OpenAIRE

    Faride Tabatabaee; Ali Mortazavi; A. Koocheki; SH. Afsharian; Behrooz Alizadeh Behbahani

    2013-01-01

    The ancient-Iranians were familiar with many medicinal herbs and were aware of their usefulness in treatment of various diseases. In this study Thymus vulgaris L., Ziziphora tenuior L. and Mentha Spicata L. extracted with methanol 96°and the antimicrobial effects of extracts were evaluated on Escherichia coli PTCC 1330 and Staphylococcus aureus PTCC 1337 by “using the method of Collins” and “disk agar diffusion method”. The results show that methanolic extracts were quite effective in 2000 μg...

  14. Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-β-farnesene

    OpenAIRE

    Crock, John; Wildung, Mark; Croteau, Rodney

    1997-01-01

    (E)-β-Farnesene is a sesquiterpene semiochemical that is used extensively by both plants and insects for communication. This acyclic olefin is found in the essential oil of peppermint (Mentha x piperita) and can be synthesized from farnesyl diphosphate by a cell-free extract of peppermint secretory gland cells. A cDNA from peppermint encoding (E)-β-farnesene synthase was cloned by random sequencing of an oil gland library and was expressed in Escherichia coli. The corresponding synthase has a...

  15. GC-MS ANALYSIS AND ANTI-MICROBIAL ACTIVITY OF ESSENTIAL OIL OF MENTHA PIPERITA L. FROM KULLU-A NORTH INDIAN REGION OF HIGHER ALTITUDE HIMALAYAS

    OpenAIRE

    SHARMA VIVEK; SHARMA NISHA; MALIK A. REYAZ; GUPTA C. RAGHBIR; PATHANIA VIJAYLATA; SINGH BIKRAM

    2010-01-01

    The essential oil analysis of Mentha piperita L. (Peppermint) has been done for the first time from study area of Northern Indian region of Kullu (1362m) district of Himachal Pradesh. The extraction yield for the essential oil of M. piperita L. was 0.42% for sample M-7. The oil was analyzed by GC-MS, the components of oil were identified by comparing their retention indices and mass spectra fragmentation patterns with those stored on the MS-computer library and also from the published literat...

  16. Avaliação da atividade antimicrobiana e antioxidante das espécies Plectranthus amboinicus (Lour.) e Mentha x villosa (Huds.)

    OpenAIRE

    Rosangela Correia Freitas; Rhuanna Rackel de Sá Azevedo; Larissa Isabela Oliveira Souza; Thiago José Matos Rocha; Aldenir Feitosa dos Santos

    2014-01-01

    O uso de plantas medicinais com potencial terapêutico tem motivado a realização de estudos através de ensaios experimentais que visam fornecer informações úteis e de extrema importância. Assim o presente trabalho objetivou avaliar a atividade antimicrobiana e antioxidante das espécies de Coleus amboinicus (Lour.) e Mentha x villosa (Huds). A atividade antimicrobiana foi avaliada pelo método de difusão em disco, cepas bacterianas padronizadas Gram negativas Escherichia ...

  17. 薄荷属植物选种育种研究进展%Research progress on seed selection and breeding of Mentha's plants

    Institute of Scientific and Technical Information of China (English)

    周露; 谢文申

    2012-01-01

    综述了薄荷属植物挥发性成分的主要化学类型及近年来国内外薄荷属植物在选种育种方面的研究进展及发展趋势.希望对促进我国现阶段薄荷属植物的选育种工作有所帮助.%In this paper, the main chemical types of the volatility components in Mentha are summarized, and the research progress and developing tendency on seed selection and breeding of the plants are reviewed.

  18. Reseach on Screening of Effective Antiviral Parts of Mentha Haplocalyx%薄荷抗单纯疱疹病毒有效部位筛选研究

    Institute of Scientific and Technical Information of China (English)

    陈飞; 姚梅悦; 张霞; 周长征

    2015-01-01

    目的:用大孔树脂吸附法提取薄荷的12个化学部位进行体外抗单纯疱疹病毒(HSV-1)研究,以确定薄荷抗单纯疱疹病毒的具体有效部位。方法:把薄荷的水煎液浓缩至一定体积上样于大孔吸附树脂柱,分别用水、25%乙醇、50%乙醇、75%乙醇进行洗脱,采用HSV-1-Hep-2(其中Hep-2为人喉癌上皮细胞)细胞体外病毒感染模型,进行体外抗HSV-1作用评价,通过显微镜下观察细胞病变效应(CPE),以酶标仪测定中性红染色A540值,计算治疗指数(TI)。结果:薄荷大孔树脂水洗脱液2号具有显著的抗HSV-1活性,其TI指数为74.3。结论:薄荷水溶性成分对HSV-1病毒有明显的抑制作用。%Objective:To determine the effective anti-herpes simplex virus(HSV) part of mentha haplocalyx by anti-HSV research in vitro on 12 chemical parts of mentha haplocalyx extracted by macroporous resin ad-sorption. Methods:The water decoction of mentha haplocalyx was concentrated to certain volume and the samples was put on the macropore adsorption resin. The samples was washed by water,25% ethanol,50%ethanol and 75% ethanol. The in vitro effect of anti HSV-1 was evaluated by in vitro infection model of HSV-1-Hep 2 cells. The cytopathic effect(CPE) was observed by microscope,the neutral red staining A540 value was determined by ELIASA and the therapeutic index(TI)was calculated. Results:The NO.2 water elu-tion has remarkable anti HSV-1 effect and the TI was 74.3. Conclusion:The water soluble ingredients of mentha haplocalyx has remarkable intibitory effect on HSV-1.

  19. Effects of essential oil of Satureja bachtiarica, Satureja khuzestanica and Mentha longifolia on the growth of Actococcus garvieae in rainbow trout fillet

    OpenAIRE

    Ansari, Mahsa

    2014-01-01

    The present research was done with the aim of studing the effects of some medicinal plant essential oils against Lactococcus garvieae and on some spoilage indexes in rainbow trout fillets in 4°C during 18 days. Essential oils of Mentha longifola (20, 40 and 80 μg g-1), Satureja bachtiarica (0.125, 0.25 and 0.50 μg g-1) and Satrureja khuzestanica (0.06, 0.12, 0.25 and 0.50 μg g-1) were used for this study. According to the results, the highest and the lowest microbial load were observed in con...

  20. In-vivo Antioxidant Effects of Ethyl Acetate Fraction of Mentha spicata L. on 4-Nitroquinoline-1-Oxide Injected Mice.

    Science.gov (United States)

    Arumugam, Ponnan; Ramesh, Arabandi

    2011-01-01

    Antioxidant effects of ethyl acetate fraction of Mentha spicata (L.) were evaluated against 4-nitroquinoline-1-oxide injected mice. For this study, experiment setup consisted of 36 albino mice of either sex divided into 6 groups: Control (25% DMSO in water), ethyl acetate fraction (EAF) alone group (80, 160 mg/Kg body weight-bwt), 4-NQO (7.5 mg/Kg bwt-IP) alone and 4-NQO + EAF. EAF and vehicles were administered orally for five consecutive days. 4-NQO (7.5 mg/Kg bwt) was injected intraperitoneally on the 6(th) day. After 24 h, the animals were killed; liver sample was extracted and used for bio-assay. 4-NQO alone treated group decreased (27-60%) the antioxidant activities and promoted lipid peroxidation (LPO-60%) over their respective control values. Pretreatment with EAF, at the maximum dose (160 mg/Kg bwt) brought down the LPO up to 87% enhanced by 4-NQO. Among the enzymatic antioxidants, glutathione S-transferase (GST) was the most affected enzyme with 4-NQO and the least was catalase (CAT). Pretreatment with EAF (160 mg/Kg bwt), the restoration of antioxidants like glutathione peroxidase, superoxide dismutase, and CAT were found equal or less than 1.2 fold higher than that of the respective control values whereas, GST was observed to be the most restored antioxidant. Be reduced glutathione (GSH) and the least vitamin C over their control values. EAF restored the GSH and Vitamin E levels were found to be 1.2 fold higher than the respective control values. PMID:24250414

  1. Effects of salinity stress on some yield parameters and morphological characteristics of spearmint (Mentha spicata L. in hydroponic conditions

    Directory of Open Access Journals (Sweden)

    Z. Safari Mohamadiyeh

    2015-11-01

    Full Text Available Mint is an important medicinal herb that is cultivated in different parts of the world. In order to investigate the effects of salinity stress on morphological characteristics of spearmint (Mentha spicata L., an experiment was conducted in 2013, based on complete randomized design, under hydroponic conditions, at Research Greenhouse of Faculty of Agriculture, Ferdowsi University of Mashhad, Iran. Treatments were five salinity levels (0, 30, 60, 90 and 120 mM of sodium chloride with four replications. Growth medium was 2:1 cocopeat to perlite, and complete nutrients solution was used. Supplying plants’ nutrients was twice a week along the irrigation water application. When plants’ height was 30 cm, salinity treatments were applied and were continued till full flowering. At the end of the experiment, morphologic traits including plant height, number of main stems, number of lateral branches, leaf area, specific leaf weight, stem diameter, number of nodes, fourth internode length, and spike length were measured. Results showed that salinity stress had significant effects on leaf area, fresh and dry weights of leaf, stem and root, plant height, number of lateral branches, number of nodes, fourth internode length, and spike length. The highest leaf area, leaf dry weight, plant height and number of lateral branches were observed in control treatment. The lowest leaf area and leaf dry weight were obtained in salinity level of 120 mM, and the lowest plant height and number of lateral branches were observed in salinity levels of 90 mM and 120 mM. Salinity had no significant effect on fresh weight of shoot to root ratio, fresh weight of leaf to root ratio, specific weight of leaves, number of main stems and stem diameter. According to the results, it appears that spearmint is sensitive to salinity stress and it can't tolerate salinity levels higher than 30 mM sodium chloride.

  2. ACTIVIDAD ENZIMÁTICA Y CAPACIDAD ANTIOXIDANTE EN MENTA (Mentha piperita L. ALMACENADA BAJO REFRIGERACIÓN

    Directory of Open Access Journals (Sweden)

    Mar\\u00EDa Teresa Mart\\u00EDnez-Dami\\u00E1n

    2013-01-01

    Full Text Available El objetivo de esta investigación fue estudiar el efecto de las bajas temperaturas sobre la actividad enzimática y capacidad antioxidante en menta (Mentha piperita L.. Se cuantificó su comportamiento en almacenamiento a 6 y 10ºC con respecto a un testigo (temperatura ambiente, para esto, se condujo un experimento en laboratorio durante el ciclo primavera-verano de 2011 en la Universidad Autónoma Chapingo, Texcoco, México. Se evaluó la actividad enzimática de peroxidasa (POD, polifenol oxidasa (PFO, superóxido dismutasa (SOD y catalasa (CAT, de igual forma se determinó la capacidad antioxidante (CA, contenido de fenoles totales (FT y de vitamina C (VC. La temperatura de 10ºC incrementó la actividad enzimática de POD de 194,72 a 306,29 U/g de PF; así como la actividad antioxidante de 262,57 a 327,32 mm TEAC/g de PF. Por otra parte, a 6 y 10ºC aumentaron los contenidos de FT y VC, inverso a lo sucedido con la actividad de SOD que disminuyó de 53,68 a 26,22 U/g de PF. El tratamiento a temperatura ambiente favoreció una mayor actividad de PFO (90,02 U/g de PF. La refrigeración incrementó la actividad enzimática de POD, el contenido de FT y VC. Por otro lado, la actividad de SOD disminuyó y la CA fue mayormente afectada por la temperatura de almacenamiento a 10ºC.

  3. Antinociceptive effects of the essential oil of Mentha x villosa leaf and its major constituent piperitenone oxide in mice

    Directory of Open Access Journals (Sweden)

    P.J.C. Sousa

    2009-07-01

    Full Text Available Mentha x villosa Huds (Labiatae is an aromatic herb widely used in folk medicine. Since the essential oil of the herb has many pharmacological activities, including antispasmodic effects, we determined whether the oil and its major constituent, piperitenone oxide (PO, have antinociceptive activity. The essential oil of M. x villosa (EOMV and PO administered orally at 200 mg/kg (vehicle: 0.1% Tween 80 in water significantly reduced the writhings induced by acetic acid from control values of 59.5 ± 3.1 s (N = 10 to 31.9 ± 2.8 s (N = 10 and 23.8 ± 3.4 s (N = 10, respectively. When administered at 100 and 200 mg/kg, EOMV reduced the paw licking time for the second phase of the formalin test from the control value of 20.6 ± 2.1 s (N = 13 to 5.3 ± 2.2 s (N = 12 and 2.7 ± 1.2 s (N = 18, respectively. At 100 and 200 mg/kg, PO reduced this second phase to 8.3 ± 2.7 s (N = 12 and 3.0 ± 1.2 s (N = 10, respectively. This effect of EOMV and PO was not reversed by naloxone. EOMV and PO had no significant effect on the first phase of the formalin test. As evaluated by the hot-plate and tail immersion test, EOMV and PO, at doses up to 200 mg/kg, showed no analgesic activity. These results show that EOMV and PO have antinociceptive activity and suggest that this effect is probably an indirect anti-inflammatory effect, which does not involve the central nervous system.

  4. Triterpenóides pentacíclicos de Mentha villosa: identificação estrutural e atribuição dos deslocamentos químicos dos átomos de hidrogênio e carbono

    OpenAIRE

    Monte Francisco J. Queiroz; Oliveira Eliete F. de; Braz Filho Raimundo

    2001-01-01

    The structures of seven oleanene and ursene triterpenoids (1-7) isolated from aerial parts of Mentha villosa were identified. In addition, the complete ¹H and 13C resonance assignments of these triterpenoids were accomplished using 1D and 2D NMR spectroscopic experiments.

  5. Comparative study of chemistry compositions and antimicrobial potentials of essential oils and oleoresins from dried and freshMentha longifolia L

    Institute of Scientific and Technical Information of China (English)

    Sunita Singh; Shiv Saran Das; Gurdip Singh; Marina Perotti; Carola Schuff; Csar Cataln

    2015-01-01

    Objective:To investigate the chemical compositions and antimicrobial potentials of the essential oils and oleoresins obtained from fresh and driedMentha longifolia L. Methods: Gas chromatography and gas chromatography-mass spectrometer techniques were used to determine the profiling of the essential oils and oleoresins. In order to determine the antimicrobial efficacy of the volatile oil and oleoresins, the pathogenic fungusAspergillus niger (1884),Aspergillus flavus (2479),Fusarium monoliforme (1893),Fusarium graminearum (2088) andPenicillium viridicatum (2007) were undertaken whereas four pathogenic bacteria Bacillus subtilis (1790),Staphylococcus aureus (3103) (Gram-positive),Escherichia coli (1672),Pseudomonas aeruginosa (1942) (Gram-negative) were selected for the present study. Food poisoned, inverted Petri plate, agar well diffusion and disk diffusion methods were employed for investigating antimicrobial potentials. Results:Piperitenone oxide, an oxygenated monoterpene, dominated the chemical compositions of essential oils and oleoresins whose compositions varied from 23.5%–87.8%. Both essential oils showed good antifungal activities againstAspergillus andFusarium species. The antibacterial investigations revealed that Gram-positive bacteria were more sensitive to the essential oils. Conclusions: Drying the fresh herbal materials influences the chemical contents and the biological activities of the essential oils and oleoresins. Such results indicate that essential oils ofMentha longifolia L. can be possible candidates for further investigations to isolate and characterize their active principles as possible new natural preservatives.

  6. Automatic Germination Evaluation and Qualitative Analysis of Essential Oil of Mentha × piperita L. under the Influence of High Frequency Pulsatile Electromagnetic and Ultrasound Pulsatile Fields

    Directory of Open Access Journals (Sweden)

    Valentin SINGUREANU

    2015-04-01

    Full Text Available The study illustrates the influence of high frequency pulsatile electromagnetic fields and ultrasound pulsatile fields on Mentha × piperita L. seed germination and the quality of its essential oil. The physiological role of the above mentioned experimental factors was considered to be a catalyticall base point, improving germination percent, SVI (seedling vigor index, GVI (germination velocity index. All the biometric aspects of the germination process (seed area, seed perimeter, seed development on x and y radius, radicele length, hypocotyl length where determined using open free software, consolidating the general idea that scientific communities can improve and perfect open source projects. High frequency pulsatile electromagnetic fields (91.75% and ultrasound pulsatile fields (64.75% experimental variants gave higher germination percent compared to control (47.00%. Following the main terpenes determination, the same experimental variants obtained high accumulations of menthol, eugenol, thymol, eucalyptol, linalool and other components. These aspects can be scientifically sustained by the seedling vigor index marks obtained at high frequency pulsatile electromagnetic fields (1985.47 and ultrasound pulsatile fields (1480.09, creating the general premises for better development stages in the nursery sector. Raised accumulation of main therapeutical terpenes in Mentha × piperita L. must be supervised in further studies, when microscopically imagery of glandular trichomes and their density may lead to more profound conclusions.

  7. Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene

    Energy Technology Data Exchange (ETDEWEB)

    Croteau, Rodney Bruce (Pullman, WA); Wildung, Mark Raymond (Colfax, WA); Crock, John E. (Moscow, ID)

    1999-01-01

    A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.

  8. Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene

    Science.gov (United States)

    Croteau, Rodney Bruce; Crock, John E.

    2005-01-25

    A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-famesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-famesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.

  9. Hemato - Immunological and biochemical parameters, skin antibacterial activity, and survival in rainbow trout (Oncorhynchus mykiss) following the diet supplemented with Mentha piperita against Yersinia ruckeri.

    Science.gov (United States)

    Adel, Milad; Pourgholam, Reza; Zorriehzahra, Jalil; Ghiasi, Maryam

    2016-08-01

    This study was aimed to assess the potential effects of Mentha piperita on the hemato - immunological and biochemical parameters, skin antibacterial activity and protection against Yersinia ruckeri infection in rainbow trout Oncorhynchus mykiss. Fish were divided into 4 groups before being fed diets supplemented with 0, 1, 2 and 3% of Mentha piperita (MP) plant extract for 8 weeks. Dose-dependent increases immune (both in skin mucus and blood serum) and hematological parameters (number of red and white cells, hematocrit and hemoglobin contents), as well as in respiratory burst activity, total protein, albumin, and neutrophil levels in fish fed supplemented diets compared to the control fish. Furthermore, dietary MP plant extract supplements have no significant effect on blood biochemical parameters and enzymatic activities of liver determined in serum of rainbow trout. After 8 weeks the cessation of feeding with MP plant extract, survival rates of 54.4%, 63.6% and 75.2% were recorded in groups which received 1, 2 and 3% of MP plant extract of feed, respectively, compared to 34.6% survivals in the control. This study underlying several positive effects of dietary administration of MP plant extract to farmed fish.

  10. Treatment of experimental pythiosis with essential oils of Origanum vulgare and Mentha piperita singly, in association and in combination with immunotherapy.

    Science.gov (United States)

    Fonseca, Anelise O S; Pereira, Daniela I B; Botton, Sônia A; Pötter, Luciana; Sallis, Elisa S V; Júnior, Sérgio F V; Filho, Fernando S M; Zambrano, Cristina Gomes; Maroneze, Beatriz P; Valente, Julia S S; Baptista, Cristiane T; Braga, Caroline Q; Ben, Vanessa Dal; Meireles, Mario C A

    2015-08-01

    This study investigated the in vivo antimicrobial activity of the essential oils of Origanum vulgare and Mentha piperita both singly, associated and in combination with immunotherapy to treat experimental pythiosis. The disease was reproduced in 18 rabbits divided into six groups (n=3): group 1, control; group 2, treated with essential oil of Mentha piperita; group 3, treated with essential oil of Origanum vulgare; group 4, treated with commercial immunotherapic; group 5, treated with a association of oils of M. piperita and O. vulgare and group 6, treated with a combination of both oils plus immunotherapy. Essential oils were added in a topical cream base formula, and lesions were treated daily for 45 days. The animals in groups 4 and 6 received a dose of immunotherapeutic agent every 14 days. The results revealed that the evolution of lesions in groups 5 and 6 did not differ from one another but differed from the other groups. The lesions of group 5 increased 3.16 times every measurement, while those of group 6 increased 1.83 times, indicating that the smallest growth of the lesions occurred when the combination of therapies were used. A rabbit from group 5 showed clinical cure at day 20 of treatment. This research is the pioneer in the treatment of experimental pythiosis using essential oils from medicinal plants and a combination of therapies. This study demonstrated that the use of essential oils can be a viable alternative treatment to cutaneous pythiosis, particularly when used in association or combination with immunotherapy.

  11. Hemato - Immunological and biochemical parameters, skin antibacterial activity, and survival in rainbow trout (Oncorhynchus mykiss) following the diet supplemented with Mentha piperita against Yersinia ruckeri.

    Science.gov (United States)

    Adel, Milad; Pourgholam, Reza; Zorriehzahra, Jalil; Ghiasi, Maryam

    2016-08-01

    This study was aimed to assess the potential effects of Mentha piperita on the hemato - immunological and biochemical parameters, skin antibacterial activity and protection against Yersinia ruckeri infection in rainbow trout Oncorhynchus mykiss. Fish were divided into 4 groups before being fed diets supplemented with 0, 1, 2 and 3% of Mentha piperita (MP) plant extract for 8 weeks. Dose-dependent increases immune (both in skin mucus and blood serum) and hematological parameters (number of red and white cells, hematocrit and hemoglobin contents), as well as in respiratory burst activity, total protein, albumin, and neutrophil levels in fish fed supplemented diets compared to the control fish. Furthermore, dietary MP plant extract supplements have no significant effect on blood biochemical parameters and enzymatic activities of liver determined in serum of rainbow trout. After 8 weeks the cessation of feeding with MP plant extract, survival rates of 54.4%, 63.6% and 75.2% were recorded in groups which received 1, 2 and 3% of MP plant extract of feed, respectively, compared to 34.6% survivals in the control. This study underlying several positive effects of dietary administration of MP plant extract to farmed fish. PMID:27245867

  12. Advances in Studies on Varieties, Harvest Processing and Packaging Storage of Menthae Haplocalycis Herba%薄荷品种、采收加工和包装贮藏研究概况

    Institute of Scientific and Technical Information of China (English)

    王文凯; 贾静; 张正; 龚千锋; 钟凌云; 张金莲; 易炳学

    2012-01-01

    对薄荷近年来品种来源、采收加工炮制、包装贮藏保管方面的研究进行文献整理和分析.以薄荷、采收、包装和贮藏为主题词检索CNKI和万方数据库的相关文献,对其中19篇文献进行了归纳分析.簿荷药材、饮片的质量与其品种来源、采收加工炮制、包装贮藏保管有密切关系.薄荷采用正确的采收加工炮制方法、进行良好的包装贮藏保管,对提高薄荷药材及饮片质量,保证临床药效有着重要的意义.%This article has sorted and analyzed the literature regarding the recent studies on Menthae Haplocalycis Herba covering its sources of varieties, harvest processing and packaging storage. Subject terms such as Menthae Haplocalycis Herba , harvesting, packaging and storage are used as a keyword to retrieve the related literature from CNK.I and Wanfang Data, 19 articles of which are summarized and analyzed. The quality of medicinal substances and decoction pieces of Menthae Haplocalycis Herba is closely related to its sources of varieties, harvest processing and packaging storage. The proper harvest processing and good packaging storage of Menthae Haplocalycis Herba is very significant to the improvement of the quality of medicinal substances and decoction pieces of Menthae Haplocalycis Herba and to the guarantee of clinical efficacy.

  13. Chemical composition and larvicidal evaluation of Mentha, Salvia, and Melissa essential oils against the West Nile virus mosquito Culex pipiens.

    Science.gov (United States)

    Koliopoulos, George; Pitarokili, Danae; Kioulos, Elias; Michaelakis, Antonios; Tzakou, Olga

    2010-07-01

    The volatile metabolites of wild-growing Mentha spicata, M. longifolia, M. suaveolens, Melissa officinalis, Salvia fruticosa, S. pomifera subsp. calycina, and S. pomifera subsp. pomifera from Greece were determined by gas chromatography and gas chromatography-mass spectrometry. The insecticidal properties of the analyzed essential oils were screened on Culex pipiens larvae. Additionally two of the main components of the essential oils, piperitenone oxide and 1,8-cineole were assayed against C. pipiens in order to define the affiliation between them and the larvicidal properties of the oils. The most effective oils were M. suaveolens (major constituent piperitenone oxide, 62.4%), M. spicata (piperitenone oxide, 35.7% and 1,8-cineole, 14.5%) and M. longifolia--Central Greece (piperitenone oxide, 33.4%; 1,8-cineole, 24.5% and trans-piperitone epoxide, 17.4%), which exhibited LC(50) values ranging from 47.88 to 59.33 mg l(-1). Medium activity revealed the oils of M. officinalis (terpin-4-ol, 15.8%; caryophyllene oxide, 13.2%; sabinene, 12.9%; beta-pinene, 12.1%; and trans-caryophyllene, 10.2%), M. longifolia--Southern Greece (carvone, 54.7% and limonene 20.0%), S. pomifera subsp. pomifera (trans-caryophyllene, 22.5% and trans-thujone, 21.0%), S. pomifera subsp. calycina--West Southern Greece (trans-thujone, 56.1% and 1,8-cineole, 10.4%), and S. fruticosa--population 2 (camphor, 23.1%; alpha-pinene, 12.7%; and borneol, 12.6%), with LC(50) values ranging from 78.28 to 91.45 mg l(-1). S. pomifera subsp. calycina (Central Greece) essential oil (trans-thujone, 26.5% and cis-thujone, 12.0%) presented rather low activity (LC(50) values 140.42 mg l(-1)), while S. fruticosa--population 1 (1,8-cineole, 31.4% and camphor, 22.6%) was the only inactive oil. Additionally, the constituent piperitenone oxide was found to be highly active (LC(50) values 9.95 mg l(-1)), whereas 1,8-cineole revealed no toxicity. PMID:20405142

  14. Morphologic alterations on red blood cells labeled with technetium-99m: the effect of Mentha crispa L. (hortela) extract

    International Nuclear Information System (INIS)

    The use of natural products, as medicinal plants, is very frequent in the world. Mentha crispa L. (M. crispa) is utilized in herbal medicine. Blood elements labeled with technetium-99m (99mTc) are used in nuclear medicine procedures and this labeling process may be altered by drugs. We have investigated the possibility of M. crispa extract being capable to alter the labeling of blood elements with 99mTc. Blood was incubated with M. crispa extract in various concentrations (6.25, 12.5, 25, 50 and 100%). Stannous chloride solution and Tc-99m, as sodium pertechnetate, were added. Blood was centrifuged and plasma (P) and blood cells (BC) were isolated. Samples of P and BC were also precipitated, centrifuged and insoluble (IF) and soluble (SF) separated. The percentage of radioactivity (%ATI) in BC, IF-P and IF-BC was calculated. Histological evaluations of the red blood cells (RBC) were performed with blood samples treated with various concentrations of M. Crispa L. and the morphology of the RBC was observed under optical microscope. Important morphological alterations expressed by mean of the perimeter/area of the RBC treated with M. crispa: 6.25% (0.67 ± 0.02), 12.5% (0.77 ± 0.03), 25% (0.73 ± 0.04), 50% (0.76 ± 0.04), 100% (0.69 ± 0.08) and the control cells (0.67 ± 0.05). The %ATI decreased: (i) on BC from 97.3 ± 1.92 to 60.0 ± 2.44; (ii) on IF-P from 74.8 ± 3.78 to 9.99 ± 3.61; (iii) on IF-BC from 88.6 ± 5.41 to 58.4 ± 11.55. The perimeter/area of the RBC showed significant differences (P>0.01) when compared 6.25% and 12.5%, and when compared 6.25% and 50% of M. Crispa L. extract. These findings could also justify the decrease of the labeling of BC with 99mTc in presence of M. Crispa extract

  15. Diferentes concentrações de solução nutritiva para o cultivo de Mentha piperita e Melissa officinalis Mentha piperita (peppermint and Melissa officinalis (balm development in different concentrations of nutritive solution

    Directory of Open Access Journals (Sweden)

    Lenita L. Haber

    2005-12-01

    Full Text Available Avaliou-se o desenvolvimento das espécies de plantas medicinais, aromáticas e condimentares Mentha piperita (hortelã-pimenta e Melissa officinalis (melissa em diversas concentrações de solução nutritiva em sistema hidropônico NFT. Os experimentos foram implementados em ambiente protegido sendo as espécies semeadas em espuma fenólica com dimensões de 2,5x2,5x3 cm, com 3 sementes por cubo, irrigadas diariamente com água até a germinação e, posteriormente com solução nutritiva diluída em 50%. Após 15 dias para a melissa e 16 dias para a hortelã-pimenta, as mudas foram transferidas para as bancadas de desenvolvimento onde receberam solução nutritiva diluída em 75% por 12 dias, sendo as mudas posteriormente transplantadas para as bancadas de cultivo onde foram submetidas às concentrações da solução nutritiva até o momento da colheita. O delineamento experimental utilizado foi inteiramente casualizado, em esquema de parcelas subdivididas. As concentrações da solução nutritiva (I 50%, II 75%, III 100%, IV 125% foram sorteadas nas parcelas e nas subparcelas avaliando-se o efeito da posição das plantas nos perfis hidropônicos (I - inicial, II - intermediária e III - final, totalizando 12 tratamentos, para cada espécie. Cada posição continha 5 conjuntos de plantas sendo utilizadas 3 repetições por tratamento. Foram avaliados a altura das plantas, número de folhas e brotos, peso fresco e seco, tanto da parte aérea quanto de raiz. Com relação à posição das plantas nos perfis, foi observada interação significativa entre os tratamentos para número de folhas e peso seco para menta e melissa, respectivamente, onde a posição inicial revelou ser o melhor tratamento. Ainda para melissa foi observada diferença significativa entre as posições para altura das plantas, peso fresco de raiz e peso fresco e seco de folhas, com a posição inicial tendo os maiores valores, apesar de não diferir significativamente

  16. 铬胁迫对薄荷生理特性的影响%Effect of Chromium Stress on Physiological Characteristics of Mentha haplocalyx Briq

    Institute of Scientific and Technical Information of China (English)

    张昌存; 高洁

    2011-01-01

    A experiment of pot culture was carried out to investigate the effects of chromium on root system vigor, chlorophyll content, antioxidant enzymes activities and MDA content of Mentha haplocalyx Briq.. It was observed that with the increasing of chromium concentrations and time, root system vigor decreased continuously; on the contrary, MDA content of different organs increased; antioxidant enzymes activities reached a peak at 40 mg/L of chromium, then decreased; chlorophyll content increased then decreased with the increasing of chromium concentrations. The results suggested that antioxidant enzymes might play important roles to Mentha haplocalyx Briq. to chromium stress. Above 40 mg/L of chromium, the antioxidant enzymes would be destroyed and the growth of plants would be bad.%为探讨薄荷(Mentha haplocalyx Briq.)耐受和富集重金属铬的生理机制,采用盆栽法研究不同质量浓度、不同时间Cr6+处理对薄荷根系活力、叶绿素含量、不同器官中抗氧化酶(SOD、POD、CAT)活性和丙二醛(MDA)含量的影响.结果表明,随着Cr6+质量浓度梯度和时间的增加,薄荷根系活力与对照相比,呈下降趋势;相反,MDA含量呈增加趋势.叶绿素a、叶绿素b和类胡萝卜素含量随Cr6+质量浓度的增加呈先升后降的趋势.SOD、POD、CAT 3种酶活性随Cr6+质量浓度的增加呈先升后降的趋势,在Cr6+质量浓度为40mg/L时,三者均出现峰值,3种酶活性随时间呈增加趋势.在Cr6+质量浓度超过40mg/L时,薄荷的抗氧化酶系统受到损害,无法对铬胁迫做出积极的响应,植株生长受到影响.

  17. Yarpuz (Mentha pulegium L. Bitkisindeki Malondialdehit, Glutatyon ve Vitamin Miktarları ile Total Antioksidan Kapasitesinin Araştırılması

    Directory of Open Access Journals (Sweden)

    Ebru ÇÖTELİ

    2014-07-01

    Full Text Available In this study, the amounts of malondialdehyde (MDA, reduced form glutathione 1, riboflavin (vitamin B2, nicotinic acid (vitamin B3, pyridoxine hydrochloride (vitamin B6 and folic acid (vitamin B9 in leaves of Mentha pulegium L. sample were determined by using High Performance Liquid Chromatography. Also, total phenolic and flavonoid contents of plant samples were determined by spectrophotometric measurements. In addition to these, the free radical scavenging effect of the plant extract on 2,2-diphenyl-1-pikrilhidrazil (DPPH, this effect was measured using the standard the antioxidant known butylated hydroxytoluene (BHT and compared. The amount of MDA, GSH, GSSG and vitamin A, vitamin E, β-carotene, vitamin C, vitamin B1, vitamin B2, vitamin B3, vitamin B6 and vitamin B9 in leaves of Mentha pulegium L. were obtained to be 17,43±1,57g/g; 185,71±10,61 g/g; 280,48±24,58 g/g; 0,38±0,08 g/g; 12,46±1,82 g/g; 1,24±0,14 g/g; 282,86±9,24 g/g; 0,50±0,09 g/g; 43,11±7,02 g/g; 16,91±1,59 g/g; 81,88±9,47 g/g and 1,53±0,16 g/grespectively. The amount of total phenolic the as 136,85±11,39 (mg/g gallic acid equivalent and the amount of the total flavanoid the 65,2±1,07 (mg/g quercetin equivalent in samples were determined. Free radical scavenging effect of 85,94±1,20 % was determined as, but compared to BHT was not significant difference (p> 0.05. It may be concluded from the results that rich, the the Mentha pulegium L. plant contains of glutathione (GSH, GSSG, vitamin C, vitamin B2 and vitamin B6, also amount contains phenolic and flavonoid content a powerful antioxidant

  18. 金牡感冒片中薄荷油的β-环糊精包合工艺研究%Studies on the IncIusion Processes by β-CycIodextrin for Oleum Menthae in Jinmu Ganmao Tablets

    Institute of Scientific and Technical Information of China (English)

    洪绯

    2015-01-01

    OBJECTIVE To study the optimal preparation technology and stability of β-cyclodextrin inclusion complex for oleum menthae in Jinmu Ganmao Tablet .METHODS The β-cyclodextrin inclusion complex in oleum menthae of Jinmu Ganmao TabletS was prepared by homogenization technique .The formulation and processing param-eters were optimized by uniform design table with utilization ratio of oleum menthae as index .The stability of oleum menthae β-cyclodextrin inclusion was studied under stressed conditions .RESULTS Under optimal preparation con-ditions for inclusion ,the ratio between oleum menthae and β-cyclodextrin was 1:10 ( mL· g-1 ) ,that between β-cy-clodextrin and water was 1:4 ,the inclusion temperature was 40℃,the inclusion time was 35 min ,and the rotate speed was 6000 r· min-1 .utilization ratio of oleum menthae was 83.1%,which was prepared under the optimal preparation conditions.Stability experiments show that the inclusion complex has certain resistance to light , heat, wet stabili-ty.CONCLUSION The optimum inclusion process features simple operation and stable inclusion complex .%目的 研究金牡感冒片中薄荷油β-环糊精包合物的最佳制备工艺并考察其稳定性. 方法 采用匀质法制备金牡感冒片薄荷油β-环糊精包合物. 以薄荷油包合利用率为评价指标,采用均匀设计法优化包合物的制备工艺与处方,并考察其稳定性. 结果 最佳包合工艺为挥发油与β-环糊精比例为1:10,β-环糊精与水比例为1:4,包合温度为 40℃,包合时间为 35min,匀质机转速为 6000rpm,薄荷油包合利用率达83.1%. 稳定性实验表明,包合物具有一定的抗光照、热、湿稳定性. 结论 试验得出的最佳包合工艺操作简便,包合物稳定.

  19. Study on Thin-layer Chromatography Separation and Anti-bacterial Activity of Mentha spicata Linn Essential Oil%留兰香挥发油的薄层分离及抗菌活性研究

    Institute of Scientific and Technical Information of China (English)

    张晓伟; 李青雨; 王德国; 石倩倩; 王佳华

    2011-01-01

    [目的]对留兰香挥发油的组分及抗菌活性进行研究.[方法]采用薄层色谱法对留兰香挥发油的组分进行初步分离,采用扩散法及紫外可见分光光度法对留兰香挥发油的抗菌活性进行测定.[结果]以石油醚:乙酸乙酯(V:V=6:1)为层析液时,留兰香挥发油的分离效果最好,分离出4种主要物质;留兰香精油对3种试验菌株的抑菌性为枯草芽孢杆菌(Bacillus subtdis)>金黄色葡萄球菌(Staphylococcus aureus)>大肠杆菌(Escherichia coli);对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌的最低抑菌浓度(MIC)分别为:0.50,1.00,3.50μl/ml.[结论]留兰香挥发油具有抑菌作用,这为综合开发留兰香提供了理论基础.%[ Objective ] To study the composition and the anti-bacterial activity of volatile oil from Mentha spicata Linn. [ Method ] Using thin-layer chromatography ( TLC ), the compositions of essential oil from Mentha spicata Linn were separated. The inhibitory activity was determined by diffusion method combining with UV spectrophotometry. [ Result] Using petroleum ether- ethyl acetate ( V: V =6:1 ) as chromatography liquid, the essential oil from Mentha spicata Linn was best in separation and four components were obtained. The inhibitory effect of volatile oil from Mentha spicata Linn to three test strains was determined to be Bacillus subtilis > Staphylococcus aureus > Escherichia coli with MIC value of 0.50, 1.00 and 3.50 ul/ml, respectively. [ Conclusion ] The volatile oil from Mentha spicata Linn had anti-bacterial effect, which provided theoretical basis for comprehensive development of Mentha spicata Linn.

  20. Isolation, fractionation and identification of chemical constituents from the leaves crude extracts of Mentha piperita L grown in Sultanate of Oman

    Institute of Scientific and Technical Information of China (English)

    Mohammad Amzad Hossain; Seham Salim Al-Hdhrami; Afaf Mohammed Weli; Qasim Al-Riyami; Jamal Nasser Al-Sabahi

    2014-01-01

    Objective: To analyze and identify the chemical compositions of different organic plants crude extracts of Mentha piperita (M. piperita) grown in Sultanate of Oman by gas chromatography-mass spectrometry (GC-MS). Methods: The powder sample was extracted with methanol by using Soxhlet extractor. Methanol crude extracts of M. piperita and its derived fractions of hexane, chloroform, ethyl acetate and butanol were prepared.Results:MS showed that majority of these compounds are bioactive.Conclusions:According to the results of the present study, the plant crude extracts could be Qualitative analyses of various organic plant crude extracts of M. piperita by using GC-used as medicine for the treatment of different diseases. The analysis and identification of the chemical compounds in the plant crude extracts by using GC-MS was the first time.

  1. UTJECAJ TEMPERATURE RADNOG MEDIJA U POSTUPKU SUŠENJA LISTA PAPRENE METVICE (Mentha piperita) NA KOLIČINU ETERIČNOG ULJA

    OpenAIRE

    S. Pliestić; Dobričević, Nadica; Filipović, D.

    2007-01-01

    List paprene metvice (Mentha piperita) nakon vaganja bio je sušen u elementarnom (tankom) sloju u sušnici na temperaturama zraka 350C, 450C i 600C. Eterično ulje izdvojeno je postupkom destilacije primjenom Clevengerovog uređaja i potom analizirano primjenom plinske kromatografije i masene spektrofotometrije (GC-MS). Količina eteričnog ulja značajno se smanjuje porastom temperature sušenja, od 1,23 % (v/v) kod temperature sušenja od 350C, na 0,78% (450C), odnosno 0,18% (60°C). Početna vlažnos...

  2. Influence of the leaf extract of mentha arvensis linn. (Mint) on the survival of mice exposed to different doses of gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Jagetia, G.C.; Baliga, M.S. [Kasturba Medical Coll., Manipal (India). Dept. of Radiobiology

    2002-02-01

    Background: The aim of the present study was to evaluate the radioprotective effect of Mentha arvensis (mint) on the survival of mice exposed to various doses of whole-body gamma radiation. Material and Methods: The radioprotective effect of various doses (0, 2.5, 5, 10, 20, 40 and 80 mg/kg body weight) of chloroform extract of mint (Mentha arvensis Linn.) was studied in mice exposed to 10 Gy gamma radiation. Results: The 10 mg/kg of mint extract was found to afford best protection as evidenced by the highest number of survivors in this group at 30 days post-irradiation, and further experiments were carried out using this dose of mint extract. The mice treated with 10 mg/kg body weight mint extract or oil were exposed to 6, 7, 8, 9 and 10 Gy of gamma radiation and observed for the induction of radiation-sickness and mortality up to 30 days post-irradiation. The mint extract pretreatment was found to reduce the severity of symptoms of radiation sickness and mortality at all exposure doses and a significant increase in the animal survival was observed when compared with the oil + irradiation group. All of the animals that were treated with 10 mg/kg mint extract and then exposed to 7 Gy irradiation were protected against the radiation-induced mortality when compared with the concurrent oil + irradiation group, in which 20% animals died by 30 days post-irradiation. The mint extract treatment protected the mice against the gastrointestinal death as well as bone marrow deaths. The DRF was found to be 1.2. The drug was non-toxic up to a dose of 1 000 mg/kg body weight, the highest drug dose that could be tested for acute toxicity. Conclusion: From our study it is clear that mint extract provides protection against the radiation-induced sickness and mortality and the optimum protective dose of 10 mg/kg is safe from the point of drug-induced toxicity. (orig.) [German] Hintergrund: Ziel der vorliegenden Studie war es, den radioprotektiven Effekt von Mentha arvensis (Minze

  3. Determination of antimutagenic properties of apigenin-7-O-rutinoside, a flavonoid isolated from Mentha longifolia (L.) Huds. ssp. longifolia with yeast DEL assay.

    Science.gov (United States)

    Gulluce, Medine; Orhan, Furkan; Adiguzel, Ahmet; Bal, Tugba; Guvenalp, Zuhal; Dermirezer, Lutfiye Omur

    2013-07-01

    Lamiaceae is an important plant family that has been investigated for its medicinal properties due to its large amounts of phenolic acids and flavonoids. Flavonoids have been shown to have antioxidant and antimutagenic activities in different test systems, but their certain mechanisms are still unclear. This study was designed to evaluate the mutagenic and antimutagenic activities of apigenin 7-O-rutinoside, a flavonoid isolated from Mentha longifolia (L.) Huds. ssp. longifolia. The possible antimutagenic potential of apigenin 7-O-rutinoside (A7R) was examined against mutagens ethyl methanesulfonate (EMS) and acridine (AC) in a eukaryotic cell system Saccharomyces cerevisiae RS112. The results showed that A7R has different inhibition rates against EMS and AC-induced mutagenicity. Thus, the properties of A7R are of great pharmacological importance and might be beneficial for reducing the risk of reactive oxygen species-related diseases.

  4. 椒样薄荷挥发油化学成分分析%Analysis on Chemical Components of Volatile Oil from Mentha piperita L.

    Institute of Scientific and Technical Information of China (English)

    陆长根; 梁呈元; 李维林

    2008-01-01

    采用水蒸气蒸馏法从椒样薄荷(Mentha piperita L.)中提取挥发油,并运用气相色谱-质谱联用技术对其化学成分进行了分析.从椒样薄荷挥发油中鉴定出46种化学成分,占总出峰面积的97.982%.其中含量较高的为薄荷醇(32.533%)、薄荷酮(23.577%)和3,7,7-三甲基-二环[4,1,0]庚烷(10.379%).

  5. Genetic variability, character association, and path analysis for economic traits in menthofuran rich half-sib seed progeny of Mentha piperita L.

    Science.gov (United States)

    Kumar, Birendra; Mali, Himanshi; Gupta, Ekta

    2014-01-01

    Menthofuran rich eight half-sib seed progeny of Mentha piperita (MPS-36) were studied for various genetic parameters, namely, coefficient of variation, heritability, genetic advance, correlation, and path of various plant and oil attributes, namely, plant height, L:S ratio, herb yield, β -myrcene, limonene, 1,8-cineole, menthone, menthofuran, neomenthone, pulegone, and menthol. Maximum genotypic coefficient of variation and genetic advance as percentage of mean were recorded for pulegone, followed by menthofuran and 1,8-cineole. The genotypic correlation in general was higher than phenotypic; positive significant correlation was recorded for limonene with 1,8-cineole and menthone, β -myrcene with limonene, and 1,8-cineole and menthofuran with neomenthol. A high direct positive effect on menthofuran was of neomenthol.

  6. Azoto normų įtaka pirmamečių pipirmėčių (Mentha piperita L.) derliui ir kokybei

    OpenAIRE

    Dambrauskienė, Edita; Bundinienė, Ona; Viškelis, Pranas; Zalatorius, Vytautas

    2007-01-01

    2006 metais Lietuvos sodininkystės ir daržininkystės instituto bandymų laukuose tirta azoto normų įtaka pipirmėčių (Mentha piperita L.) žalios masės derliui ir kokybei. Visos tyrimams naudotos pipirmėtės: varietetas iš Lietuvos, varietetas iš Lenkijos ir ‘Krasnodarskaja’ veislė, papildomai patręštos azoto trąšomis, derėjo gausiau. Tyrimais nustatyta, kad didžiausias žalios masės derliaus priedas gautas, tręšiant pipirmėtes didžiausia azoto norma N120 per 3 kartus (N60+30+30). Pipirmėčių žalio...

  7. Effect of aqueous extracts of Mentha arvensis (mint) and Piper betle (betel) on growth and citrinin production from toxigenic Penicillium citrinum.

    Science.gov (United States)

    Panda, Pragyanshree; Aiko, Visenuo; Mehta, Alka

    2015-06-01

    Due to growing concern of consumers about chemical residues in food products, the demand for safe and natural food is increasing greatly. The use of natural additives such as spices and herbal oil as seasoning agents for their antimicrobial activity has been extensively investigated. This paper discusses the efficacy of the aqueous extract of mint (Mentha arvensis) and betel (Piper betle) on the mycelial growth and citrinin production of Penicillium citrinum. The present investigation revealed that mint extract inhibited citrinin production up to 73 % without inhibiting the mycelium growth. The citrinin production decreased with increase in the concentration of mint extract as observed from the data obtained from High pressure liquid chromatography. The samples also showed reduced cytotoxicity on HeLa cells. On the other hand betel extract resulted in stimulatory effect on citrinin production and mycelial growth. The study showed that mint extract has the potential to be used safely for restraining citrinin contamination.

  8. In vitro evaluation of schistosomicidal activity of essential oil of Mentha x villosa and some of its chemical constituents in adult worms of Schistosoma mansoni.

    Science.gov (United States)

    Matos-Rocha, Thiago José; dos Santos Cavalcanti, Marília Gabriela; Barbosa-Filho, José Maria; Lúcio, Ana Silvia Suassuna Carneiro; Veras, Dyana Leal; Feitosa, Ana Paula Sampaio; de Siqueira Júnior, José Pinto; de Almeida, Reinaldo Nóbrega; Marques, Márcia Ortiz Mayo; Alves, Luiz Carlos; Brayner, Fábio André

    2013-09-01

    This study aimed to determine the composition of the essential oil of Mentha x villosa and to evaluate its biological effects in vitro on adult worms of S. mansoni. Rotundifolone (70.96 %), limonene (8.75 %), trans-caryophyllene (1.46 %), and β-pinene (0.81 %) were shown to be the major constituents of this oil. Adult worms of S. mansoni were incubated with different concentrations of the essential oil (1, 10, 100, 250, 500, and 1000 µg/mL) and of its constituents rotundifolone (0.7, 3.54, 7.09, 70.96, 177.4, 354.8, and 700.96 µg/mL), limonene (43.75 µg/mL), trans-caryophyllene (7.3 µg/mL), and β-pinene (4.03 µg/mL). No schistosomicidal activity was identified at the trans-caryophyllene and β-pinene concentrations studied. However, use of the essential oil (10 µg/mL), rotundifolone (7.09 µg/mL), and limonene (43.75 µg/mL) resulted in decreased worm motility continuing until 96 hours of observation. At higher concentrations (100 and 70.96 µg/mL, respectively), both the essential oil and rotundifolone caused mortality among adult worms of S. mansoni. The positive control praziquantel caused the death of all parasites after 24 h of evaluation. The results from this study suggest that the essential oil of Mentha x villosa presents schistosomicidal efficacy.

  9. Treatment of experimental pythiosis with essential oils of Origanum vulgare and Mentha piperita singly, in association and in combination with immunotherapy.

    Science.gov (United States)

    Fonseca, Anelise O S; Pereira, Daniela I B; Botton, Sônia A; Pötter, Luciana; Sallis, Elisa S V; Júnior, Sérgio F V; Filho, Fernando S M; Zambrano, Cristina Gomes; Maroneze, Beatriz P; Valente, Julia S S; Baptista, Cristiane T; Braga, Caroline Q; Ben, Vanessa Dal; Meireles, Mario C A

    2015-08-01

    This study investigated the in vivo antimicrobial activity of the essential oils of Origanum vulgare and Mentha piperita both singly, associated and in combination with immunotherapy to treat experimental pythiosis. The disease was reproduced in 18 rabbits divided into six groups (n=3): group 1, control; group 2, treated with essential oil of Mentha piperita; group 3, treated with essential oil of Origanum vulgare; group 4, treated with commercial immunotherapic; group 5, treated with a association of oils of M. piperita and O. vulgare and group 6, treated with a combination of both oils plus immunotherapy. Essential oils were added in a topical cream base formula, and lesions were treated daily for 45 days. The animals in groups 4 and 6 received a dose of immunotherapeutic agent every 14 days. The results revealed that the evolution of lesions in groups 5 and 6 did not differ from one another but differed from the other groups. The lesions of group 5 increased 3.16 times every measurement, while those of group 6 increased 1.83 times, indicating that the smallest growth of the lesions occurred when the combination of therapies were used. A rabbit from group 5 showed clinical cure at day 20 of treatment. This research is the pioneer in the treatment of experimental pythiosis using essential oils from medicinal plants and a combination of therapies. This study demonstrated that the use of essential oils can be a viable alternative treatment to cutaneous pythiosis, particularly when used in association or combination with immunotherapy. PMID:26036789

  10. Citocininas na multiplicação in vitro de hortelã-pimenta (Mentha x Piperita L. Cytokinins on in vitro multiplication of peppermint (Mentha x Piperita L.

    Directory of Open Access Journals (Sweden)

    S. A. Asmar

    2011-01-01

    Full Text Available O hortelã-pimenta é um hibrido triplo, Mentha x piperita, utilizado de forma medicinal no tratamento de náuseas, cólicas gastrointestinais, flatulências, cálculos biliares, icterícia, ansiedade, expectoração e expulsão de vermes intestinais. Na micropropagação, o BAP (6-benzilaminopurina e KIN (cinetina têm sido as fontes de citocininas mais empregadas. O objetivo deste trabalho foi avaliar o efeito de reguladores vegetais na multiplicação in vitro de hortelã-pimenta. Explantes constituídos de segmentos nodais provenientes de plântulas já estabelecidas in vitro com aproximadamente 0,5 cm foram inoculados em meio MS, suplementado com diferentes associações de BAP e KIN, em adição de 30 g L-1 de sacarose. As concentrações de citocininas utilizadas foram 0,0; 1,0 e 2,0 mg L-1 de BAP e 0,0; 0,5 e 1,0 mg L-1 de KIN. O delineamento experimental foi o inteiramente casualizado, com nove tratamentos consistindo de cinco frascos cada um, sendo que cada frasco continha quatro explantes. A utilização de 2,0 mg L-1 de BAP promove a multiplicação in vitro de hortelã-pimenta, porém, diminui o índice de sobrevivência. O uso de citocininas aumenta a massa fresca e seca dos explantes e a ausência destes reguladores propicia o alongamento nesta espécie.Peppermint is used for medical treatments of nausea, gastrointestinal cramps, flatulence, gallstones, jaundice, anxiety, sputum and expulsion of intestinal worms. In micropropagation, BAP (6-benzylaminopurine and KIN (kinetin have been the most widely used sources of cytokinins. The aim of this study was to evaluate the effect of plant growth regulators in multiplication of peppermint. Explants consisting of nodal segments from seedlings already established in vitro with approximately 0,5 cm were inoculated on MS medium supplemented with different combinations of BAP and KIN and 30 g L-1 of sucrose. The concentrations of cytokinins used were 0,0; 1,0 and 2,0 mg L-1 of BAP and 0

  11. Relationship analysis of Mentha species based on RAPD marker%基于RAPD标记的薄荷属(Mentha L.)植物亲缘关系分析

    Institute of Scientific and Technical Information of China (English)

    房海灵; 李维林; 梁呈元; 郭巧生

    2010-01-01

    应用RAPD标记方法分析了薄荷属(Mentha L. )7个种38个种源间的遗传多样性,并采用UPGMA聚类分析方法探讨了38个种源间的亲缘关系.结果表明,用20条随机引物从38个种源的总DNA中共扩增出111条带,其中多态性条带91条,多态性条带百分率达81.98%,表明薄荷属植物种间和种内存在丰富的遗传多样性.聚类分析结果表明,在遗传相似系数0.43 处,供试的38个种源被分为2大类,其中第1大类包含日本薄荷(M. arvensis L. )、灰薄荷(M. vagans Boriss. )、留兰香(M. spicata L. )、皱叶留兰香(M. crispata Schrad. ex Willd. )、椒样薄荷(M.×piperita L. )和薄荷(M. haplocalyx Briq. )的37个种源,第2大类仅包含唇萼薄荷(M. pulegium L. )1个种源.在遗传相似系数0.74处,38个种源被分为6组:A组仅包含日本薄荷1个种源;B组包含灰薄荷的4个种源;C组包含留兰香的2个种源和皱叶留兰香的6个种源;D组包含椒样薄荷的5个种源和留兰香的2个种源;E组包含薄荷的17个种源;F组仅包含唇萼薄荷1个种源.在遗传相似系数0.83处,B组、C组、D组和E组可各自进一步划分为不同的亚组.研究结果显示,基于RAPD标记分析的聚类分析结果与传统形态学分类结果基本相吻合;同一种类来源相同或相近的种源聚在一起,说明薄荷属植物种内的遗传关系与地理分布和环境差异有一定的相关性.

  12. Zhodnocení vlivu některých faktorů na uchovatelnost čerstvých řezaných rostlin máty klasnaté (Mentha spicata)

    OpenAIRE

    Bourová, Kristýna

    2012-01-01

    The aim of this bachelor work was to evaluate the effect of the production system , after-harvest treatment and the temperature on the preservation of newly-cut plants of spearmint (Mentha spicata L.). The main observed factors were the effect of the production system (ecological and conventional), effect of the temperature during stocking in cooling boxes (6 and 8°C) and the impact of after-harvest treatment (saturation in hydrogen peroxide solution and glucose of various c...

  13. Classification and Recognition of Mentha Based On the Generalized Regression Neural Network%基于广义回归神经网络的薄荷属植物的分类识别

    Institute of Scientific and Technical Information of China (English)

    黄劲潮

    2015-01-01

    Mentha is easily to hybrid that polymorphism phenomenon is serious, therefore, the classification of Mentha mainly uses classification method or the number of classification and so on, but it spends a lot of time and effort of these methods. This paper selects some strong related attributes of plant category distinctions of Mentha as the training set, constructs the Generalized Regression Neural Network with mentors to classify and recognize Mentha, and ensures the accuracy and the recognition speed. At the same time, the model can also be used to tell whether some certain attribute of Mint is strongly related with classification recognition or not.%薄荷属植物易杂交以致多型性现象严重,对于薄荷属植物的分类主要采用分类检索表法或数量分类法等,但这些方法花费的时间与物力较大。选取了薄荷属植物类别区分的一些强相关属性作为训练组,构建了广义回归神经网络来对薄荷属植物进行分类、识别,在保证准确度的同时也兼顾了识别的速度。同时,该模型也可用来识别薄荷属植物的某种属性是否与类别区分强相关。

  14. 基于广义回归神经网络的薄荷属植物的分类识别%Classification and Recognition of Mentha Based On the Generalized Regression Neural Network

    Institute of Scientific and Technical Information of China (English)

    黄劲潮

    2015-01-01

    Mentha is easily to hybrid that polymorphism phenomenon is serious, therefore, the classification of Mentha mainly uses classification method or the number of classification and so on, but it spends a lot of time and effort of these methods. This paper selects some strong related attributes of plant category distinctions of Mentha as the training set, constructs the Generalized Regression Neural Network with mentors to classify and recognize Mentha, and ensures the accuracy and the recognition speed. At the same time, the model can also be used to tell whether some certain attribute of Mint is strongly related with classification recognition or not.%薄荷属植物易杂交以致多型性现象严重,对于薄荷属植物的分类主要采用分类检索表法或数量分类法等,但这些方法花费的时间与物力较大。选取了薄荷属植物类别区分的一些强相关属性作为训练组,构建了广义回归神经网络来对薄荷属植物进行分类、识别,在保证准确度的同时也兼顾了识别的速度。同时,该模型也可用来识别薄荷属植物的某种属性是否与类别区分强相关。

  15. Synthetic Photochemistry. LIII. Low-Temperature Irradiation of Methyl 2,4-Dioxopentanoate with alpha-Phellandrene, p-Mentha-1,5-diene. Product Distribution and Further Occurrence of "retro-Benzilic Acid Rearrengement"

    OpenAIRE

    Hatsui, Toshihide; Nojima, Chitoshi; Takeshita, Hitoshi

    1989-01-01

    Low-temperature irradiation of methyl 2,4-dioxopentanoate with p-mentha-1,5-diene (alpha-phellandrene) gave retro-benzilic acid rearrangement products in low but reproducible yields, after a pyrolytic workup, together with isomeric [4+2] cycloadducts. This indicates an accumulation of the proto-photocycloadducts during the reaction. The product analysis indicated the [2+2] and [4+2] cycloadditions are mutually independent processes.

  16. In vitro study on the antimicrobial effect of hydroalcoholic extracts from Mentha arvensis L. (Lamiaceae) against oral pathogens - doi: 10.4025/actascibiolsci.v34i4.8959

    OpenAIRE

    Francisco Augusto Burkert Del Pino; Gladis Aver Ribeiro; Rogério Antonio Freitag; Patrícia da Silva Nascente; Rosana Serpa; Rafael Guerra Lund

    2012-01-01

    In vitro tests could be a valuable tool for the evaluation of medicinal plants’ antimicrobial activity. Mentha arvensis of the Lamiaceae family is one of the most frequently traditional plants used in Brazil. Hydroalcoholic extracts of M. arvensis were analyzed for antimicrobial activity on Streptococcus mutans, Streptococcus sobrinus and Candida albicans. Three different assays (agar diffusion, broth macro- and micro-dilution methods) were used to evaluate antimicrobial activity. Although hy...

  17. Mycorrhizal fungi inoculation and phosphorus fertilizer on growth, essential oil production and nutrient uptake in peppermint (Mentha piperita L.) Inoculação com fungos micorrízicos e adubação fosfatada no crescimento, produção de óleo essencial e absorção de nutrientes em hortelã-pimenta (Mentha piperita L.)

    OpenAIRE

    M.C. Arango; M.F. Ruscitti; M.G. Ronco; J. Beltrano

    2012-01-01

    This study evaluated the effects of inoculation with the arbuscular mycorrhizal fungi Glomus mosseae, Glomus intraradices A4 and Glomus intraradices B1 and two phosphorus levels (10 and 40 mg kg-1) on root colonization, plant growth, nutrient uptake and essential oil content in Mentha piperita L. The experiment was carried out in a greenhouse, in 4x2 factorial arrangement, in completely randomized design. At sixty days after transplanting, the mycorrhizal plants had significantly higher fresh...

  18. Research Development on Chemical Composition in Herba Menthae Related with Efficacy and Toxicity%基于功效和毒性的薄荷化学成分研究进展

    Institute of Scientific and Technical Information of China (English)

    何希望; 李晓宇; 孙蓉

    2011-01-01

    Objective Summarizing the current states of chemical composition of Herba Menthae related with its efficacy and toxicity, in order to identify its effective and toxical composition and offer the accordance and research ideas for further research on the relationship between efficacy, toxicity and chemical composition of Herba Menthae. Methods Ancient books and the related literatures in the recent years of Herba Mentnae were collated, analyzed and summarized. Results Volatile oil are both the main material basis for efficacy and toxicity in Herba Menthae; the research about efficacy, material basis and toxicity of Herba Menthae is isolated and short of relevance. The research for its basis of extraction and separation technology research and quality control is also seldom-reported. Conclusion As a common-used record of TCM, Herba Menthae is urgent to be studied, based on the efficacy and chemicals related basic and "keep- efficacy" and "control- toxicity" research to provide experimental basement and literature reference for rational use in clinic.%目的 总结与薄荷的功效、毒性相关的化学成分研究现状,明确薄荷的有效成分和毒性成分,为进一步研究薄荷功效与毒性的相关性提供文献依据和研究思路.方法 对历史古籍和近些年的相关文献进行整理、分析、归纳.结果 薄荷挥发油既是薄荷的功效物质基础也是毒性物质基础.目前针对薄荷的功效、毒性和物质基础研究都是孤立的,彼此之间缺少关联性,毒性机制还不完善.针对药效物质基础和毒性物质基础的提取、分离工艺优化研究与质量控制研究也鲜有报道.结论 作为临床常用药薄荷,亟待需要进行基于功效和毒性相关的化学物质基础研究,为临床安全、可控、有效使用薄荷提供试验依据和文献思路.

  19. Influência do cultivo em consórcio na produção de fitomassa e óleo essencial de manjericão (Ocimum basilicum L.) e hortelã (Mentha x villosa Huds.) Influence of intercropping on phytomass and essential oil production in basil (Ocimum basilicum L.) and mint (Mentha x villosa Huds.)

    OpenAIRE

    J.T.L.S. Maia; Martins, E. R.; Costa, C. A.; E.O.F. Ferraz; I.C.A. Alvarenga; I.T. Souza Júnior; S.V. Valadares

    2009-01-01

    As plantas medicinais produzem seus princípios ativos de acordo com os estímulos do ambiente, por isso a presença de outras plantas pode comprometer a produção dos metabólicos secundários. Assim, o presente trabalho teve como objetivo avaliar o efeito do cultivo consorciado sobre a produção de fitomassa e o teor de óleo essencial de manjericão (Ocimum basilicum L.) e hortelã (Mentha x villosa Huds.). O experimento foi conduzido na área experimental do Núcleo de Ciências Agrárias da Universida...

  20. Desenvolvimento vegetativo de Mentha campestris Schur e produção de mentol em diferentes espaçamentos de plantio e épocas de colheita Vegetative development of Mentha campestris Schur and menthol production in different row spaces and harvest times

    Directory of Open Access Journals (Sweden)

    R. Monteiro

    2011-01-01

    Full Text Available A produção de óleos essenciais nas plantas aromáticas é influenciada por fatores bióticos e abióticos. A demanda por esses produtos tem aumentado, sendo os óleos essenciais do gênero Mentha de grande interesse nas indústrias farmacêutica, de cosméticos, alimentícia e agrícola, principalmente em função do composto mentol. Esse trabalho teve como objetivo avaliar o efeito de três espaçamentos de plantio (0,60 x 0,15 m; 0,60 x 0,30 m e 0,60 x 0,45 m e duas épocas de colheita (60 e 90 dias após o plantio na espécie Mentha campestris Schur. O experimento foi conduzido no Centro de Estações Experimentais do Canguiri-UFPR, em Pinhais-PR, no período de janeiro a abril de 2008. O delineamento utilizado foi o de blocos ao acaso em esquema de parcelas subdivididas. Houve diferença significativa para todas as variáveis analisadas. As massas secas de folhas, ramos e total foram maiores que na primeira época. Para a biomassa seca de folhas foram observados maiores valores no menor espaçamento de plantio. O rendimento de óleo essencial foi maior na segunda época de colheita e nos espaçamentos maiores. A produtividade do óleo também foi maior na segunda época de colheita, porém no espaçamento mais adensado. Pode-se concluir como recomendação para M. campestris Schur o espaçamento 0,60 x 0,15 m e colheita aos 90 dias, por terem atingido maior biomassa, rendimento de óleo essencial e produtividade de mentol por hectare.Essential oil production in aromatic plants is influenced by biotic and abiotic factors. The demand for these products has increased, and essential oils from the genus Mentha have been of great interest for pharmaceutical, cosmetic, food and agronomic industries, especially because of the compound menthol. This study aimed to evaluate the effect of three row spaces (0.60 x 0.15 m; 0.60 x 0.30 m and 0.60 x 0.45 m and two harvest times (60 and 90 days after planting on the species Mentha campestris Schur. The

  1. Protective effects of solvent fractions of Mentha spicata (L. leaves evaluated on 4-nitroquinoline-1-oxide induced chromosome damage and apoptosis in mouse bone marrow cells

    Directory of Open Access Journals (Sweden)

    Ponnan Arumugam

    2009-01-01

    Full Text Available Spearmint leaves (Mentha spicata L. contain high levels of antioxidants that are known to protect against both exogenous and endogenous DNA damage. In this study, the protective effects of the hexane fraction (HF, chloroform fraction (CF and ethyl acetate fraction (EAF in an ethanol extract from M. spicata were evaluated against 4-nitroquinoline-1-oxide (4-NQO induced chromosome damage and apoptosis in bone marrow cells of Swiss albino mice. Two (EAF; 80 and 160 mg/ kg body weight - bw or three (HF and CF; 80, 160 and 320 mg/ kg bw doses of solvent fractions or vehicle control (25% DMSO in water were administered orally for five consecutive days. Upon the sixth day, 4-NQO was injected intraperitoneally. The animals were killed the following day. Other control groups were comprised of animals treated with either the vehicle control or the various doses of solvent fractions, but with no 4-NQO treatment. 4-NQO induced micro-nucleated polychromatic erythrocytes (MnPCEs in all the test groups. However, pre-treatment of animals with the solvent fractions significantly reduced the 4-NQO-induced MnPCEs as well as the percentage of apoptotic cells. The reduction of both MnPCE and apoptosis was more evident following the pre-treatment of animals with 160 mg/kg bw EAF.

  2. Protective effects of solvent fractions of Mentha spicata (L.) leaves evaluated on 4-nitroquinoline-1-oxide induced chromosome damage and apoptosis in mouse bone marrow cells.

    Science.gov (United States)

    Arumugam, Ponnan; Ramesh, Arabandi

    2009-10-01

    Spearmint leaves (Mentha spicata L.) contain high levels of antioxidants that are known to protect against both exogenous and endogenous DNA damage. In this study, the protective effects of the hexane fraction (HF), chloroform fraction (CF) and ethyl acetate fraction (EAF) in an ethanol extract from M. spicata were evaluated against 4-nitroquinoline-1-oxide (4-NQO) induced chromosome damage and apoptosis in bone marrow cells of Swiss albino mice. Two (EAF; 80 and 160 mg/ kg body weight - bw) or three (HF and CF; 80, 160 and 320 mg/ kg bw) doses of solvent fractions or vehicle control (25% DMSO in water) were administered orally for five consecutive days. Upon the sixth day, 4-NQO was injected intraperitoneally. The animals were killed the following day. Other control groups were comprised of animals treated with either the vehicle control or the various doses of solvent fractions, but with no 4-NQO treatment. 4-NQO induced micro-nucleated polychromatic erythrocytes (MnPCEs) in all the test groups. However, pre-treatment of animals with the solvent fractions significantly reduced the 4-NQO-induced MnPCEs as well as the percentage of apoptotic cells. The reduction of both MnPCE and apoptosis was more evident following the pre-treatment of animals with 160 mg/kg bw EAF. PMID:21637463

  3. Effect of Mentha spicata L. and Artemisia campestris extracts on the shelf life and quality of vacuum-packed refrigerated sardine (Sardina pilchardus) fillets.

    Science.gov (United States)

    Houicher, Abderrahmane; Kuley, Esmeray; Bendeddouche, Badis; Ozogul, Fatih

    2013-10-01

    The present study investigated the effects of ethanolic extracts obtained from Mentha spicata and Artemisia campestris on the shelf life and the quality of vacuum-packed sardine fillets stored at 3 ± 1°C for a period of 21 days. The three groups were tested were VC, control group; VM, group treated with 1 % mint extract; and VA, group treated with 1 % artemisia extract. The observed shelf life of sardine fillets was 10 days for control samples, whereas the combination of vacuum packaging with mint and artemisia extracts extended the product's shelf life to 17 days. Among the chemical indices determined, the thiobarbituric acid-reactive substances values were significantly lower in VM samples. Total volatile base nitrogen was maintained at low levels in VA samples until 17 days of chilled storage. Results of aerobic plate counts and coliform counts showed the existence of a reduced growth in VA group, whereas lactic acid bacteria did not show a significant difference among groups. Natural extract treatments combined with vacuum packaging showed lower microbiological and chemical indices, indicating that the presence of phenolic compounds in mint and artemisia extracts and the removal of oxygen in the pack retarded lipid oxidation and reduced the growth of microorganisms, which resulted in preventing spoilage and extending the product's shelf life. PMID:24112571

  4. Productivity and quality of volatile oil extracted from Mentha spicata and M. arvensis var. piperascens grown by a hydroponic system using the deep flow technique.

    Science.gov (United States)

    Vimolmangkang, Sornkanok; Sitthithaworn, Worapan; Vannavanich, Danai; Keattikunpairoj, Sunisa; Chittasupho, Chuda

    2010-01-01

    The purpose of this study was to determine the differences between spearmint (Mentha spicata L.) and Japanese mint (M. arvensis L. var. piperascens Malinv.) cultivated in either soil or nutrient solution using the deep flow technique (DFT). The differences were measured in terms of harvest period (full bloom period) and quantity and chemical components of volatile oils. The spearmint and Japanese mint were cultivated in four different nutrient formulas: plant standard nutrient, plant standard nutrient with an amino acid mixture, plant standard nutrient with a sulphur compound, and a combination of plant standard nutrient with an amino acid mixture and a sulphur compound. We observed that cultivation of spearmint and Japanese mint in nutrient solution using DFT is an effective method to provide high production of volatile oil, since it results in an earlier harvest period and higher quantity of volatile oil. We determined that for spearmint an amino acid mixture is an appropriate nutrient supplement to enhance production of volatile oil with optimum carvone content. Finally, we observed high menthol content in Japanese mint grown in all four nutrient formulas; however, supplementation with a combination of sulphur fertilisation and amino acid mixture yields the highest quantity of volatile oil. PMID:19763744

  5. Studies on free-radical scavenging activity and identification of active ingredients of different plant crude extracts of Mentha piperita collected from Sur, Sultanate of Oman

    Institute of Scientific and Technical Information of China (English)

    Mohammad Amzad Hossain; Aqeela Said Hamed AL Orimi; Afaf Mohammed Weli; Qasim Al-Riyami; Jamal Nasser Al-Sabahi

    2014-01-01

    Objective: To determine free radical scavenging activity and active chemical ingredients of different plant crude extracts of Mentha piperita (M. piperita). Methods: The dried powder leaves of M. piperita were extracted with polar organic solvent by Soxhlet extractor. The crude extract and its fractions of hexane, chloroform, ethyl acetate and butanol crude extracts were prepared. The antioxidant activity of different crude extracts from M. piperita was carried out by DPPH method with minor modification, and the active chemical ingredients of different plant crude extracts of M. piperita were analyzed by gas chromatography-mass spectrometry (GC-MS). Results: Qualitative analysis of different polarities crude extracts by GC-MS found different types of active organic compounds. The antioxidant activity of different crude extracts were found to be in the order of chloroform extract> butanol extract> ethyl acetate extract> hexane extract>methanol extract. Majority identified compounds in the plant crude extracts by GC-MS were biologically active. Conclusions: Therefore, the isolation, purification, identification and characterization of bioactive compounds from various crude extracts of M. piperita might have ecological significance.

  6. Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria.

    Science.gov (United States)

    Husain, Fohad M; Ahmad, Iqbal; Khan, Mohammad S; Ahmad, Ejaz; Tahseen, Qudisa; Khan, Mohd Shahnawaz; Alshabib, Nasser A

    2015-01-01

    Bacterial quorum sensing (QS) is a density dependent communication system that regulates the expression of certain genes including production of virulence factors in many pathogens. Bioactive plant extract/compounds inhibiting QS regulated gene expression may be a potential candidate as antipathogenic drug. In this study anti-QS activity of peppermint (Mentha piperita) oil was first tested using the Chromobacterium violaceum CVO26 biosensor. Further, the findings of the present investigation revealed that peppermint oil (PMO) at sub-Minimum Inhibitory Concentrations (sub-MICs) strongly interfered with acyl homoserine lactone (AHL) regulated virulence factors and biofilm formation in Pseudomonas aeruginosa and Aeromonas hydrophila. The result of molecular docking analysis attributed the QS inhibitory activity exhibited by PMO to menthol. Assessment of ability of menthol to interfere with QS systems of various Gram-negative pathogens comprising diverse AHL molecules revealed that it reduced the AHL dependent production of violacein, virulence factors, and biofilm formation indicating broad-spectrum anti-QS activity. Using two Escherichia coli biosensors, MG4/pKDT17 and pEAL08-2, we also confirmed that menthol inhibited both the las and pqs QS systems. Further, findings of the in vivo studies with menthol on nematode model Caenorhabditis elegans showed significantly enhanced survival of the nematode. Our data identified menthol as a novel broad spectrum QS inhibitor. PMID:26029178

  7. Chemical Composition and Anti-Inflammatory, Cytotoxic and Antioxidant Activities of Essential Oil from Leaves of Mentha piperita Grown in China.

    Science.gov (United States)

    Sun, Zhenliang; Wang, Huiyan; Wang, Jing; Zhou, Lianming; Yang, Peiming

    2014-01-01

    The chemical composition, anti-inflammatory, cytotoxic and antioxidant activities of essential oil from leaves of Mentha piperita (MEO) grown in China were investigated. Using GC-MS analysis, the chemical composition of MEO was characterized, showing that it was mainly composed of menthol, menthone and menthy acetate. MEO exhibited potent anti-inflammatory activities in a croton oil-induced mouse ear edema model. It could also effectively inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. The cytotoxic effect was assessed against four human cancer cells. MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively. In addition, MEO had moderate antioxidant activity. The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources. PMID:25493616

  8. Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita

    Directory of Open Access Journals (Sweden)

    Eleni Fitsiou

    2016-08-01

    Full Text Available Natural products, known for their medicinal properties since antiquity, are continuously being studied for their biological properties. In the present study, we analyzed the composition of the volatile preparations of essential oils of the Greek plants Ocimum basilicum (sweet basil, Mentha spicata (spearmint, Pimpinella anisum (anise and Fortunella margarita (kumquat. GC/MS analyses revealed that the major components in the essential oil fractions, were carvone (85.4% in spearmint, methyl chavicol (74.9% in sweet basil, trans-anethole (88.1% in anise, and limonene (93.8% in kumquat. We further explored their biological potential by studying their antimicrobial, antioxidant and antiproliferative activities. Only the essential oils from spearmint and sweet basil demonstrated cytotoxicity against common foodborne bacteria, while all preparations were active against the fungi Saccharomyces cerevisiae and Aspergillus niger. Antioxidant evaluation by DPPH and ABTS radical scavenging activity assays revealed a variable degree of antioxidant potency. Finally, their antiproliferative potential was tested against a panel of human cancer cell lines and evaluated by using the sulforhodamine B (SRB assay. All essential oil preparations exhibited a variable degree of antiproliferative activity, depending on the cancer model used, with the most potent one being sweet basil against an in vitro model of human colon carcinoma.

  9. Investigation the effects using different levels of Mentha pulegium L. (pennyroyal) in comparison with an antibiotic growth promoter on performance, carcass traits and immune responses in broiler chickens

    Institute of Scientific and Technical Information of China (English)

    Gholamreza Ghalamkari; Majid Toghyani; Nasir Landy; Ehsan Tavalaeian

    2012-01-01

    Objective: The trial involved 240 Ross 308 broiler chicks in order to investigate the effects of Mentha pulegium L. (pennyroyal) on performance, carcass traits and immune responses in broiler chickens. Methods: Birds were assigned to 4 treatments: control feed, antibiotic group receiving 4.5 mg/kg flavophospholipol, and 5 and 10 g/kg pennyroyal powder added to the basal diet. Body weights of broilers were determined at d 1, 14, 28 and 42, feed intake was determined at the same periods, and feed conversion ratio was calculated accordingly. At day 42, two birds per replicate were slaughtered for the determination of carcass traits. Antibody titers against newcastle, influenza viruses and sheep red blood cell (SRBC) were determined. Results: Performance, Internal organ weights and carcass characteristics were not significantly influenced by the dietary treatments at day 42. Humoral immune responses were not affected by dietary treatments.Conclusions:In conclusion, the results of this study showed that addition of pennyroyal powder seem not to have a positive influence on growth performance of broiler chicks.

  10. Avaliação da atividade antimicrobiana e antioxidante das espécies Plectranthus amboinicus (Lour. e Mentha x villosa (Huds.

    Directory of Open Access Journals (Sweden)

    Rosangela Correia Freitas

    2014-01-01

    Full Text Available O uso de plantas medicinais com potencial terapêutico tem motivado a realização de estudos através de ensaios experimentais que visam fornecer informações úteis e de extrema importância. Assim o presente trabalho objetivou avaliar a atividade antimicrobiana e antioxidante das espécies de Coleus amboinicus (Lour. e Mentha x villosa (Huds. A atividade antimicrobiana foi avaliada pelo método de difusão em disco, cepas bacterianas padronizadas Gram negativas Escherichia coli (ATCC 25922 e ATCC 35218, cepas Gram positivas Staphylococcus aureus (ATCC 25923 e S. aureus ATCC 27853, assim como fungos padrões de Cryptococcus neoformans (pertencentes à coleção de microrganismos do CESMAC. Como controle positivo foi usado o imipenem e fluconazol e como controle negativo um disco com etanol absoluto. A determinação do potencial antioxidante foi realizada utilizando os métodos DPPH, FTC e determinação de compostos fenólicos. Os extratos etanólicos de C. amboinicus e M. x villosa apresentaram excelentes resultados tanto relacionado à atividade antimicrobiana quanto à antioxidante. Nossos resultados mostram a potencialidade das plantas analisadas na prevenção e no combate de doenças.

  11. Efficiency of ethanolic extract of peppermint (Mentha piperita) as an antibiotic growth promoter substitution on performance, and carcass characteristics in broiler chickens

    Institute of Scientific and Technical Information of China (English)

    Shahram Nanekarani; Majid Goodarzi; Mohammad Heidari; Nasir Landy

    2012-01-01

    Objective: This trial was conducted to examine the effect of usage different levels of ethanolic extract of peppermint (Mentha piperita) in drinking water in comparison with an antibiotic growth promoter (Virginiamycin) on the growth performance, and carcass traits of broiler chicks.Methods:virginiamycin, and 0.1, 0.2 and 0.3 % ethanolic extract of peppermint supplemented to drinking water. Body weights of broilers were determined at d 1, 21 and 42, feed intake was determined at the same periods, and feed conversion ratio was calculated accordingly. At day 42, two birds per replicate were slaughtered for the determination of carcass traits. Results: Performance indices were not significantly influenced by the dietary treatments at day 42. Carcass yield increased in broilers supplemented with 0.3 % peppermint extract in drinking water, also, abdominal fat pad decreased in broilers supplemented with 0.3 % peppermint in drinking water (P>0.05).Conclusions:Birds were assigned to 5 treatments: control feed, antibiotic group receiving 5 mg/kg In conclusion, the results of this study showed that addition of 0.3 % ethanolic extract of peppermint to drinking water seem to have a positive influence on broiler performance productive via more carcass yield and decrease abdominal fat deposition.

  12. Metal absorption properties of Mentha spicata grown under tannery sludge amended soil-its effect on antioxidant system and oil quality.

    Science.gov (United States)

    Patel, Anju; Pandey, Vineeta; Patra, D D

    2016-03-01

    Tannery sludge (TS) is hazardous to environment and its disposal in an ecofriendly manner is a major challenge. An experiment was conducted to investigate the metal absorption properties of Mentha spicata grown under different levels of TS amended soil (soil: sludge in 100:0, 75:25, 50:50, 25:75 and 0:100 ratio) and its effect on the antioxidant system and oil quality. At 75:25 ratio of sludge and soil, metal translocation factor was ≥0.5 for Cr, Cd, and Co and for Ni and for Pb ≥ 1. Carvone, limonene, dihydrocarvone and other oil constituents along with biomass were maximum in 75:25 ratio of sludge and soil. Superoxide dismutase (SOD), CAT (Catalases), POD (Peroxidases), MDA (Malondialdehyde) and proline play a major role in detoxification of reactive oxygen species generated due to TS (heavy metal stress). Antioxidant (SOD, CAT and POD), MDA and proline showed an increasing trend as the concentration of TS increased with the treatments. To test the relationship between 23 character principal component analysis (PCA) was performed. PC-I contributed 56% of total variance while PC-II contributed 37% of total variance. The results concluded that M. spicata performed well in terms of oil yield and multiple metal translocations in 75:25 sludge and soil ratio.

  13. Effect of chemical elicitors on peppermint (Mentha piperita) plants and their impact on the metabolite profile and antioxidant capacity of resulting infusions.

    Science.gov (United States)

    Figueroa Pérez, Marely G; Rocha-Guzmán, Nuria Elizabeth; Mercado-Silva, Edmundo; Loarca-Piña, Guadalupe; Reynoso-Camacho, Rosalía

    2014-08-01

    Infusions are widely consumed all over the world and are a source of dietary antioxidants, which can be improved in plants using elicitors. The aim of this study was to evaluate the foliar application of salicylic acid (SA) (0.5, 1 and 2mM) or hydrogen peroxide (H2O2) (0.05, 0.1 and 0.5mM) on peppermint (Mentha piperita) plants and its effect on the metabolite profile and antioxidant capacity of resulting infusions. Whereas 2mM SA treatment improved plant growth parameters and metabolite profile (carbohydrates and amino acids), 0.5 and 1mM SA treatments increased phenolic compound concentration. Sinapic acid, rutin and naringin were detected only in SA treatments; antioxidant capacity was also improved. Regarding H2O2 treatments, no differences in plant growth parameters, metabolite profile or antioxidant capacity were found. Therefore, the application of SA to peppermint is recommended in order to improve bioactive compounds and the antioxidant capacity of infusions.

  14. Effects of dietary peppermint (Mentha piperita) on growth performance, chemical body composition and hematological and immune parameters of fry Caspian white fish (Rutilus frisii kutum).

    Science.gov (United States)

    Adel, Milad; Abedian Amiri, Armin; Zorriehzahra, Jalil; Nematolahi, Amin; Esteban, Maria Ángeles

    2015-08-01

    Peppermint (Mentha piperita L.) is a very popular herb. While numerous effects have been described in mammals, its effects on fish have received so far limited attention. The effects of dietary administration of peppermint on fry Caspian white fish (Rutilus frisii kutum) were studied. Fish were divided into 4 groups before being fed diets supplemented with 0% (control), 1%, 2% and 3% of peppermint extracts for 8 weeks. Dose-dependent increases of growth parameters (WG and SGR), mucus skin (protein concentration, alkaline phosphatase and antimicrobial activity) and seric (lysozyme and IgM) and blood leucocyte respiratory burst activities and different hematological parameters (number of red and white cells, seric hemoglobin and hematocrit content) were recorded in fry fish fed supplemented diets. However, the dietary peppermint supplements have different effects on the number of blood leucocytes depending on the leucocyte cell type. While no significant differences were observed in the number of blood monocytes and eosinophils, the number of neutrophils and lymphocytes was increased and decreased, respectively, on fish fed peppermint enriched diets, respect to the values found in control fish. Present results corroborate that dietary administration of peppermint promotes growth performance and increases the main hematological and immune humoral (both mucosal and systemic) parameters of fry Caspian white fish. This study may provide new applications of peppermint and, at the same time, promote rational development and utilization of peppermint resources.

  15. Studies on the chemical constituents of the active parts of Mentha spicata L.(Ⅰ)%留兰香的活性成分(Ⅰ)

    Institute of Scientific and Technical Information of China (English)

    郑健; 高慧媛; 陈广通; 吴斌; 吴立军

    2006-01-01

    目的对唇形科薄荷属植物留兰香(Mentha spicata L.)的活性部位的化学成分进行研究.方法采用硅胶柱色谱等方法进行分离纯化,根据理化性质和光谱数据进行结构鉴定.结果得到4个化合物,分别鉴定为3-甲氧基-4-羟基苯甲酸(3-methoxy -4hydroxybenzoic acid,Ⅰ)、3,5-二甲氧基-4羟基苯甲酸(3,5-dimethoxy -4-hydroxybenzoic acid,Ⅱ)、橙皮素-7- O -β -D -葡萄糖苷(hesperetin -7- O -β -D -glucoside,Ⅲ)、芹菜素-7- O -β -D -吡喃葡萄糖苷(apigenin -7- O -β - D -pyranglucoside,Ⅳ).结论化合物Ⅰ,Ⅱ为首次从薄荷属植物中分离得到,化合物Ⅲ,Ⅳ为留兰香中首次分离得到.

  16. Efficacy of Mentha spicata essential oil in suppression of Aspergillus flavus and aflatoxin contamination in chickpea with particular emphasis to mode of antifungal action.

    Science.gov (United States)

    Kedia, Akash; Dwivedy, Abhishek Kumar; Jha, Dhruva Kumar; Dubey, Nawal Kishore

    2016-05-01

    The present study reports in vivo antifungal and antiaflatoxigenic efficacy of Mentha spicata essential oil (EO) against toxigenic Aspergillus flavus strain LHP(C)-D6 in chickpea food system up to 12 months of storage. In addition, the mode of antifungal action of EO was also determined to understand the mechanism of fungal growth inhibition. The in vivo study with different concentrations of M. spicata EO showed dose-dependent decrease in fungal colony count as well as aflatoxin B1 concentration. The EO caused >50% protection in inoculated sets and >70% protection in uninoculated sets of chickpea food system against A. flavus at 1.0 μL mL(-1) air concentration. However, at the same concentration, EO caused 100% inhibition to aflatoxin B1 production in both sets when analyzed through high-performance liquid chromatography (HPLC). The antifungal target of EO in fumigated cells of A. flavus was found to be the plasma membrane when analyzed through electron microscopic observations and ions leakage test. The EO fumigated chickpea seeds showed 100% seed germination and seedling growth after 12 months of storage. Based on these observations, M. spicata EO can be recommended as plant-based preservative for safe protection of food commodities during storage conditions against fungal and most importantly mycotoxin contaminations.

  17. Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita.

    Science.gov (United States)

    Fitsiou, Eleni; Mitropoulou, Gregoria; Spyridopoulou, Katerina; Tiptiri-Kourpeti, Angeliki; Vamvakias, Manolis; Bardouki, Haido; Panayiotidis, Mihalis Ι; Galanis, Alex; Kourkoutas, Yiannis; Chlichlia, Katerina; Pappa, Aglaia

    2016-01-01

    Natural products, known for their medicinal properties since antiquity, are continuously being studied for their biological properties. In the present study, we analyzed the composition of the volatile preparations of essential oils of the Greek plants Ocimum basilicum (sweet basil), Mentha spicata (spearmint), Pimpinella anisum (anise) and Fortunella margarita (kumquat). GC/MS analyses revealed that the major components in the essential oil fractions, were carvone (85.4%) in spearmint, methyl chavicol (74.9%) in sweet basil, trans-anethole (88.1%) in anise, and limonene (93.8%) in kumquat. We further explored their biological potential by studying their antimicrobial, antioxidant and antiproliferative activities. Only the essential oils from spearmint and sweet basil demonstrated cytotoxicity against common foodborne bacteria, while all preparations were active against the fungi Saccharomyces cerevisiae and Aspergillus niger. Antioxidant evaluation by DPPH and ABTS radical scavenging activity assays revealed a variable degree of antioxidant potency. Finally, their antiproliferative potential was tested against a panel of human cancer cell lines and evaluated by using the sulforhodamine B (SRB) assay. All essential oil preparations exhibited a variable degree of antiproliferative activity, depending on the cancer model used, with the most potent one being sweet basil against an in vitro model of human colon carcinoma.

  18. 留兰香活性部位化学成分的研究Ⅲ%Study of chemical constituents in active parts of Mentha spicata Ⅲ

    Institute of Scientific and Technical Information of China (English)

    陈广通; 高慧媛; 郑健; 吴斌; 杨小珂; 吴立军

    2006-01-01

    目的:对唇形科薄荷属植物留兰香Mentha spicata的活性部位化学成分进行深入研究.方法:采用硅胶柱色谱等方法进行分离纯化,根据理化性质和光谱数据进行结构鉴定.结果:分离得到5个化合物,分别鉴定为原儿茶醛(protocatechuic aldehyde,Ⅰ),原儿茶酸(protocatechuic acid,Ⅱ),3'-甲氧基木犀草素(chrysoeriol,Ⅲ),5,6-二羟基-7,8,3',4'-四甲氧基黄酮(5,6-dihydroxy-7,8,3',4'tetramethoxyflavone,Ⅳ),5,6,7,4'-四羟基-3'-甲氧基黄酮(nodifloretin,Ⅴ).结论:化合物Ⅰ,Ⅱ为首次从薄荷属植物中分离得到,化合物Ⅲ,Ⅳ,Ⅴ为留兰香中首次分离得到.

  19. Bio-preservative effect of the essential oil of the endemic Mentha piperita used alone and in combination with BacTN635 in stored minced beef meat.

    Science.gov (United States)

    Smaoui, Slim; Hsouna, Anis Ben; Lahmar, Aida; Ennouri, Karim; Mtibaa-Chakchouk, Ahlem; Sellem, Imen; Najah, Soumaya; Bouaziz, Mohamed; Mellouli, Lotfi

    2016-07-01

    The major compounds in Mentha piperita essential oil (EOMP) were menthol (33.59%) and iso-menthone (33%). The biopreservative effect of EOMP used alone at 0.25 or 0.5% and in combination with the semi-purified bacteriocin BacTN635 at 500 or 1000AU/g, on minced beef meat was evaluated by microbiological, physicochemical and sensory analyses during storage at 4°C for 21days. EOMP used alone limited the microbial deterioration of minced meat (P<0.05). Furthermore, the combination between EOMP and BacTN635 led to a decrease in TBARS values and slowed down the accumulation of MetMb. This combination was more efficient (P<0.05) against microflora proliferation and enhanced the sensory acceptability extending thus the shelf life of meat beef by approximately 7days. On the basis of these results, physicochemical and sensorial parameters could be used for constructing regression models to predict overall acceptability. Overall, the strongest preservative effect was achieved by using the combination of EOMP at 0.5% with BacTN535 at 1000AU/g.

  20. Essential oil composition and antimicrobial activity of wild and cultivated mint timija (Mentha suaveolens subsp. timija (Briq.) Harley), an endemic and threatened medicinal species in Morocco.

    Science.gov (United States)

    Kasrati, A; Jamali, C Alaoui; Bekkouche, K; Lahcen, H; Markouk, M; Wohlmuth, H; Leach, D; Abbad, A

    2013-01-01

    The hydro-distilled essential oils obtained from aerial parts of the wild (w) and cultivated (c) mint timija (Mentha suaveolens subsp. timija), an endemic medicinal species of Morocco, have been analyzed by GC-MS and screened for antimicrobial activity. In total, 35 compounds representing more than 98% of the oils were identified. Menthone (39.4(w)-10.8(c)%), pulegone (62.3(c)-34.3(w)%) and isomenthone (9.3(c)-7.8(w)%) were found as the main components for the two oils. The volatiles of the wild and cultivated material differed significantly in both the percentage of the main components and antimicrobial effect. Pulegone was more dominant in cultivated mint timija (62.3%) than in wild one (34.3%), while menthone was more abundant in the wild material (39.4%). In the antimicrobial assays, both oils displayed good to excellent activity against all microorganisms tested with the oil of the cultivated form being more active.

  1. 留兰香的活性成分(Ⅱ)%Chemical constituents of the active parts of Mentha spicata L.(Ⅱ)

    Institute of Scientific and Technical Information of China (English)

    郑健; 高慧媛; 陈广通; 杨小珂; 吴斌; 吴立军

    2006-01-01

    目的对唇形科薄荷属植物留兰香(Mentha spicata L.)的活性部位的化学成分进行研究.方法采用硅胶柱色谱等方法进行分离纯化,根据理化性质和光谱数据进行结构鉴定.结果又分离得到5个化合物,分别鉴定为(+)isolariciresinol 2a - O -β -D -glucoside (Ⅰ)、5,4' -二羟基黄酮-7- O -β -D -吡喃葡萄糖醛酸丁酯(5,4'-dihydroxy flavonoid -7- O -β -D pyranglycuronate butyl ester,Ⅱ)、香蜂草苷(didymin,Ⅲ)、橙皮苷(hesperitin,Ⅳ)、迷迭香酸(rosmarinic acid,Ⅴ).结论化合物Ⅰ、Ⅱ是从薄荷属植物中首次分离得到,化合物Ⅲ、Ⅳ、Ⅴ是从留兰香中首次分离得到.

  2. Immunological and parasitological parameters in Schistosoma mansoni-infected mice treated with crude extract from the leaves of Mentha x piperita L.

    Science.gov (United States)

    Dejani, Naiara N; Souza, Laís C; Oliveira, Sandra R P; Neris, Débora M; Rodolpho, Joice M A; Correia, Ricardo O; Rodrigues, Vanderlei; Sacramento, Luis V S; Faccioli, Lúcia H; Afonso, Ana; Anibal, Fernanda F

    2014-08-01

    Schistosomiasis is a chronic disease caused by an intravascular trematode of the genus Schistosoma. Praziquantel is the drug used for treatment of schistosomiasis; nevertheless failure of treatment has been reported. Consequently, the identification of new effective schistosomicidal compounds is essential to ensure the effective control of schistosomiasis in the future. In this work we investigated the immunomodulatory and antiparasitic effects of the crude leaves extract of Mentha x piperita L. (peppermint) on murine Schistosomiasis mansoni. Female Balb/c mice were infected each with 50 S. mansoni cercariae and divided into three experimental groups: (I) untreated; (II) treated daily with M. x piperita L. (100mg/kg) and III) treated on 1/42/43 days post-infection with Praziquantel (500mg/kg). Another group with uninfected and untreated mice was used as a control. Subsequently, seven weeks post-infection, S. mansoni eggs were counted in the feces, liver and intestine. Worms were recovered by perfusion of the hepatic portal system and counted. Sera levels of IL-10, IL-5, IL-13, IFN-γ, IgG1, IgE and IgG2a were assayed by ELISA. Animals treated with a daily dose of M. x piperita L. showed increased sera levels of IL-10, IFN-γ, IgG2a and IgE. Besides, M. x piperita L. treatment promoted reduction in parasite burden by 35.2% and significant decrease in egg counts in the feces and intestine.

  3. Induction and prolireration of Mentha haplocalyx Briq leaves callus%薄荷叶片愈伤组织的诱导与增殖

    Institute of Scientific and Technical Information of China (English)

    周伟香; 龚宁

    2006-01-01

    以薄荷(Mentha haplocalyx Briq)幼嫩叶片为外植体,采用正交设计L9(33)考察6-BA,NAA,2,4-D 3种植物生长调节物质对叶片愈伤组织诱导的影响,实验结果表明薄荷叶片愈伤组织诱导最佳培养基为MS+6-BA10mg/L+NAA1.0mg/L+2,4-D 0.5 mg/L+蔗糖30 g/L.在此培养基上研究不同培养条件对愈伤组织增殖效应的影响,结果表明薄荷愈伤组织继代增殖适合且经济的培养条件为蔗糖20 g/L,液体浅层静置培养.

  4. A Study on Chemical Constituents in the Herb of Mentha spicata%留兰香中化学成分的分离与鉴定

    Institute of Scientific and Technical Information of China (English)

    郑健; 赵东升; 吴斌; 吴立军

    2002-01-01

    目的:对唇形科薄荷属植物留兰香Mentha spicata的活性部位进行系统研究.方法:采用溶剂法和各种层析方法.结果:分离得到 7个化合物,分别鉴定为乌索烷 (ursane,I ), 3-甲氧基-4-甲基苯甲醛(3-methoxy-4-methylbenzaldehyde,Ⅱ), 藜芦酸(veratric acid,Ⅲ),5-羟基-3′,4′,6,7-四甲氧基黄酮(5-hydroxy-3′,4′,6,7-tetramethoxyflavone,trimethoxyflavone,thymonin,Ⅵ),香叶木素(diosmetin,V),5,6,4′-三羟基-7,8,3′-三甲氧基黄酮(5,6,4′-rihydroxy-7,8,3′-trimethoxyflavone,thymonin,Ⅵ),胡萝卜苷(daucosterol,Ⅶ).结论:化合物I,Ⅱ,Ⅲ是从薄荷属植物中首次分离得到,化合物Ⅳ,Ⅵ是从留兰香中首次分离得到.

  5. Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita.

    Science.gov (United States)

    Fitsiou, Eleni; Mitropoulou, Gregoria; Spyridopoulou, Katerina; Tiptiri-Kourpeti, Angeliki; Vamvakias, Manolis; Bardouki, Haido; Panayiotidis, Mihalis Ι; Galanis, Alex; Kourkoutas, Yiannis; Chlichlia, Katerina; Pappa, Aglaia

    2016-01-01

    Natural products, known for their medicinal properties since antiquity, are continuously being studied for their biological properties. In the present study, we analyzed the composition of the volatile preparations of essential oils of the Greek plants Ocimum basilicum (sweet basil), Mentha spicata (spearmint), Pimpinella anisum (anise) and Fortunella margarita (kumquat). GC/MS analyses revealed that the major components in the essential oil fractions, were carvone (85.4%) in spearmint, methyl chavicol (74.9%) in sweet basil, trans-anethole (88.1%) in anise, and limonene (93.8%) in kumquat. We further explored their biological potential by studying their antimicrobial, antioxidant and antiproliferative activities. Only the essential oils from spearmint and sweet basil demonstrated cytotoxicity against common foodborne bacteria, while all preparations were active against the fungi Saccharomyces cerevisiae and Aspergillus niger. Antioxidant evaluation by DPPH and ABTS radical scavenging activity assays revealed a variable degree of antioxidant potency. Finally, their antiproliferative potential was tested against a panel of human cancer cell lines and evaluated by using the sulforhodamine B (SRB) assay. All essential oil preparations exhibited a variable degree of antiproliferative activity, depending on the cancer model used, with the most potent one being sweet basil against an in vitro model of human colon carcinoma. PMID:27537869

  6. Bio-preservative effect of the essential oil of the endemic Mentha piperita used alone and in combination with BacTN635 in stored minced beef meat.

    Science.gov (United States)

    Smaoui, Slim; Hsouna, Anis Ben; Lahmar, Aida; Ennouri, Karim; Mtibaa-Chakchouk, Ahlem; Sellem, Imen; Najah, Soumaya; Bouaziz, Mohamed; Mellouli, Lotfi

    2016-07-01

    The major compounds in Mentha piperita essential oil (EOMP) were menthol (33.59%) and iso-menthone (33%). The biopreservative effect of EOMP used alone at 0.25 or 0.5% and in combination with the semi-purified bacteriocin BacTN635 at 500 or 1000AU/g, on minced beef meat was evaluated by microbiological, physicochemical and sensory analyses during storage at 4°C for 21days. EOMP used alone limited the microbial deterioration of minced meat (P<0.05). Furthermore, the combination between EOMP and BacTN635 led to a decrease in TBARS values and slowed down the accumulation of MetMb. This combination was more efficient (P<0.05) against microflora proliferation and enhanced the sensory acceptability extending thus the shelf life of meat beef by approximately 7days. On the basis of these results, physicochemical and sensorial parameters could be used for constructing regression models to predict overall acceptability. Overall, the strongest preservative effect was achieved by using the combination of EOMP at 0.5% with BacTN535 at 1000AU/g. PMID:26995774

  7. Efficacy of Mentha spicata essential oil in suppression of Aspergillus flavus and aflatoxin contamination in chickpea with particular emphasis to mode of antifungal action.

    Science.gov (United States)

    Kedia, Akash; Dwivedy, Abhishek Kumar; Jha, Dhruva Kumar; Dubey, Nawal Kishore

    2016-05-01

    The present study reports in vivo antifungal and antiaflatoxigenic efficacy of Mentha spicata essential oil (EO) against toxigenic Aspergillus flavus strain LHP(C)-D6 in chickpea food system up to 12 months of storage. In addition, the mode of antifungal action of EO was also determined to understand the mechanism of fungal growth inhibition. The in vivo study with different concentrations of M. spicata EO showed dose-dependent decrease in fungal colony count as well as aflatoxin B1 concentration. The EO caused >50% protection in inoculated sets and >70% protection in uninoculated sets of chickpea food system against A. flavus at 1.0 μL mL(-1) air concentration. However, at the same concentration, EO caused 100% inhibition to aflatoxin B1 production in both sets when analyzed through high-performance liquid chromatography (HPLC). The antifungal target of EO in fumigated cells of A. flavus was found to be the plasma membrane when analyzed through electron microscopic observations and ions leakage test. The EO fumigated chickpea seeds showed 100% seed germination and seedling growth after 12 months of storage. Based on these observations, M. spicata EO can be recommended as plant-based preservative for safe protection of food commodities during storage conditions against fungal and most importantly mycotoxin contaminations. PMID:26338202

  8. Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata).

    Science.gov (United States)

    Wang, Qian; Reddy, Vaishnavi Amarr; Panicker, Deepa; Mao, Hui-Zhu; Kumar, Nadimuthu; Rajan, Chakravarthy; Venkatesh, Prasanna Nori; Chua, Nam-Hai; Sarojam, Rajani

    2016-07-01

    In many aromatic plants including spearmint (Mentha spicata), the sites of secondary metabolite production are tiny specialized structures called peltate glandular trichomes (PGT). Having high commercial values, these secondary metabolites are exploited largely as flavours, fragrances and pharmaceuticals. But, knowledge about transcription factors (TFs) that regulate secondary metabolism in PGT remains elusive. Understanding the role of TFs in secondary metabolism pathway will aid in metabolic engineering for increased yield of secondary metabolites and also the development of new production techniques for valuable metabolites. Here, we isolated and functionally characterized a novel MsYABBY5 gene that is preferentially expressed in PGT of spearmint. We generated transgenic plants in which MsYABBY5 was either overexpressed or silenced using RNA interference (RNAi). Analysis of the transgenic lines showed that the reduced expression of MsYABBY5 led to increased levels of terpenes and that overexpression decreased terpene levels. Additionally, ectopic expression of MsYABBY5 in Ocimum basilicum and Nicotiana sylvestris decreased secondary metabolite production in them, suggesting that the encoded transcription factor is probably a repressor of secondary metabolism. PMID:26842602

  9. Metal absorption properties of Mentha spicata grown under tannery sludge amended soil-its effect on antioxidant system and oil quality.

    Science.gov (United States)

    Patel, Anju; Pandey, Vineeta; Patra, D D

    2016-03-01

    Tannery sludge (TS) is hazardous to environment and its disposal in an ecofriendly manner is a major challenge. An experiment was conducted to investigate the metal absorption properties of Mentha spicata grown under different levels of TS amended soil (soil: sludge in 100:0, 75:25, 50:50, 25:75 and 0:100 ratio) and its effect on the antioxidant system and oil quality. At 75:25 ratio of sludge and soil, metal translocation factor was ≥0.5 for Cr, Cd, and Co and for Ni and for Pb ≥ 1. Carvone, limonene, dihydrocarvone and other oil constituents along with biomass were maximum in 75:25 ratio of sludge and soil. Superoxide dismutase (SOD), CAT (Catalases), POD (Peroxidases), MDA (Malondialdehyde) and proline play a major role in detoxification of reactive oxygen species generated due to TS (heavy metal stress). Antioxidant (SOD, CAT and POD), MDA and proline showed an increasing trend as the concentration of TS increased with the treatments. To test the relationship between 23 character principal component analysis (PCA) was performed. PC-I contributed 56% of total variance while PC-II contributed 37% of total variance. The results concluded that M. spicata performed well in terms of oil yield and multiple metal translocations in 75:25 sludge and soil ratio. PMID:26761599

  10. Efficacy of mint (Mentha arvensis) leaf and citrus (Citrus aurantium) peel extracts as natural preservatives for shelf life extension of chill stored Indian mackerel.

    Science.gov (United States)

    Viji, Pankyamma; Binsi, Puthanpurakkal Kizhakkathil; Visnuvinayagam, Sivam; Bindu, Jaganath; Ravishankar, Chandragiri Nagarajarao; Srinivasa Gopal, Teralandur Krishnaswamy

    2015-10-01

    Efficacy of mint (Mentha arvensis) leaf and citrus (Citrus aurantium) peel extracts in retarding the quality changes in Indian mackerel during chilled storage was investigated. Mint leaf extract showed higher quantity of phenolics and superior in-vitro antioxidant activities than citrus peel extract. Gutted mackerel were given a dip treatment in mint extract (0.5 %, w/v) and citrus extract (1 % w/v), packed in LDPE pouches and stored at 0-2 °C. The biochemical quality indices viz. total volatile base nitrogen (TVB-N), trimethylamine nitrogen (TMA-N), free fattyacids (FFA) were significantly (p < 0.05) lower in mint extract (ME) treated fishes compared to citrus extract (CE) treated and control fishes (C) without any treatment. Plant extract treatment significantly inhibited lipid oxidation in mackerel as indicated by peroxide value (PV) and thiobarbituric acid reactive substances (TBARS). Aerobic plate count (APC) was markedly higher in C group followed by CE group throughout the storage period. As per sensory evaluation, shelf life of Indian mackerel was determined to be 11-13 days for C group, 13-15 days for CE group and 16-17 days for ME group, during storage at 0-2 °C. PMID:26396373

  11. Effects of dietary peppermint (Mentha piperita) on growth performance, chemical body composition and hematological and immune parameters of fry Caspian white fish (Rutilus frisii kutum).

    Science.gov (United States)

    Adel, Milad; Abedian Amiri, Armin; Zorriehzahra, Jalil; Nematolahi, Amin; Esteban, Maria Ángeles

    2015-08-01

    Peppermint (Mentha piperita L.) is a very popular herb. While numerous effects have been described in mammals, its effects on fish have received so far limited attention. The effects of dietary administration of peppermint on fry Caspian white fish (Rutilus frisii kutum) were studied. Fish were divided into 4 groups before being fed diets supplemented with 0% (control), 1%, 2% and 3% of peppermint extracts for 8 weeks. Dose-dependent increases of growth parameters (WG and SGR), mucus skin (protein concentration, alkaline phosphatase and antimicrobial activity) and seric (lysozyme and IgM) and blood leucocyte respiratory burst activities and different hematological parameters (number of red and white cells, seric hemoglobin and hematocrit content) were recorded in fry fish fed supplemented diets. However, the dietary peppermint supplements have different effects on the number of blood leucocytes depending on the leucocyte cell type. While no significant differences were observed in the number of blood monocytes and eosinophils, the number of neutrophils and lymphocytes was increased and decreased, respectively, on fish fed peppermint enriched diets, respect to the values found in control fish. Present results corroborate that dietary administration of peppermint promotes growth performance and increases the main hematological and immune humoral (both mucosal and systemic) parameters of fry Caspian white fish. This study may provide new applications of peppermint and, at the same time, promote rational development and utilization of peppermint resources. PMID:26067171

  12. Chemical Constituents in Ethyl Acetate Extracts from Mentha haplocalyx%薄荷乙酸乙酯提取部位的化学成分

    Institute of Scientific and Technical Information of China (English)

    李明亮; 徐凌玉; 李振麟; 钱士辉; 李艺; 秦民坚

    2013-01-01

    从薄荷乙酸乙酯提取部位分离鉴定了8个化合物,分别为委陵菜酸(tormentic acid,1),野椿酸(euscaphic acid,2),乌苏酸(ursolic acid,3),齐墩果酸(oleanolic acid,4),尿嘧啶(uracil,5),琥珀酸(succinic acid,6),(9E)-8,11,12-trihydroxyoctadecenoic acid methyl ester(7),neoechinulin A(8).化合物1,2,5,6为首次从该植物中分到;7和8为在唇形科中首次分到.%Eight compounds were isolated in ethyl acetate extracts from Mentha haplocalyx Briq. Their structures were identified as tormentic acid (1), euscaphic acid (2), ursolic acid (3), oleanolic acid (4), uracil (5), succinic acid (6), (9E)-8, 11, 12-trihydroxyoctadecenoic acid methyl ester (7) and neoechinulin A (8). Compounds 1, 2, 5, and 6 were isolated from this plant for the first time. Compounds 7 and 8 were the first isolated from the family of Lamicaeae.

  13. Studies on free-radical scavenging activity and identification of active ingredients of different plant crude extracts of Mentha piperita collected from Sur, Sultanate of Oman

    Directory of Open Access Journals (Sweden)

    Mohammad Amzad Hossain

    2014-10-01

    Full Text Available Objective: To determine free radical scavenging activity and active chemical ingredients of different plant crude extracts of Mentha piperita (M. piperita. Methods: The dried powder leaves of M. piperita were extracted with polar organic solvent by Soxhlet extractor. The crude extract and its fractions of hexane, chloroform, ethyl acetate and butanol crude extracts were prepared. The antioxidant activity of different crude extracts from M. piperita was carried out by DPPH method with minor modification, and the active chemical ingredients of different plant crude extracts of M. piperita were analyzed by gas chromatographymass spectrometry (GC-MS. Results: Qualitative analysis of different polarities crude extracts by GC-MS found different types of active organic compounds. The antioxidant activity of different crude extracts were found to be in the order of chloroform extract> butanol extract> ethyl acetate extract> hexane extract> methanol extract. Majority identified compounds in the plant crude extracts by GC-MS were biologically active. Conclusions: Therefore, the isolation, purification, identification and characterization of bioactive compounds from various crude extracts of M. piperita might have ecological significance.

  14. Allelopathic Effect of Aqueous Extracts from the Leaves of Peppermint (Mentha × piperita L. on Selected Physiological Processes of Common Sunflower (Helianthus annuus L.

    Directory of Open Access Journals (Sweden)

    Edyta SKRZYPEK

    2015-12-01

    Full Text Available In plants cultivation, some species influencing each other in a favorable manner, and others adversely affect the result of the release of physiologically active substances. These substances, called allelopathic compounds are excreted primarily by underground and aboveground plants’ organs or formed during the decomposition of their remains. Allelopathins show the inhibitory or stimulating effects on the processes of seed germination, growth and physiological activity of plants. The aim of the study was to determine the allelopathic effects of aqueous extracts from the peppermint (Mentha × piperita L. leaves at various concentrations (1, 3, 5, 10, 15% on seeds germination and the selected physiological processes of common sunflower (Helianthus annuus L. seedlings. Seeds were germinated and plants were grown under greenhouse conditions for 30 days. Germination of sunflower seeds was reduced and electrolyte leakage from seedlings increased with increasing concentrations of aqueous extracts of the peppermint leaves. Increasing concentrations of aqueous extracts of peppermint also caused decrease of chlorophyll a and an increase of chlorophyll b content. The highest increase of the maximum photochemical efficiency of photosystem II was observed in H. annuus L. treated with 15% peppermint extract in comparison to the lower concentration of extracts and to the control. Non-photochemical and photochemical quenching and vitality index of photosystem II decreased with increasing concentrations of allelopathic substances in peppermint extracts.

  15. GC-MS ANALYSIS AND ANTI-MICROBIAL ACTIVITY OF ESSENTIAL OIL OF MENTHA PIPERITA L. FROM KULLU-A NORTH INDIAN REGION OF HIGHER ALTITUDE HIMALAYAS

    Directory of Open Access Journals (Sweden)

    SHARMA VIVEK

    2010-12-01

    Full Text Available The essential oil analysis of Mentha piperita L. (Peppermint has been done for the first time from study area of Northern Indian region of Kullu (1362m district of Himachal Pradesh. The extraction yield for the essential oil of M. piperita L. was 0.42% for sample M-7. The oil was analyzed by GC-MS, the components of oil were identified by comparing their retention indices and mass spectra fragmentation patterns with those stored on the MS-computer library and also from the published literatures. The major constituents reported from essential oils of M. piperita were: L-Menthone (28.66%; Menthol (9.94%; Piperitone oxide (16.0%; Eucalyptol (7.03%; L-Menthone (3.13%; Isoneomenthol (2.93%; á-Phellandrene (3.21%; ä-3-Carene (3.27%; dl-Limonene (2.53%; á-Pinene (2.02%, etc. Furthermore, anti-microbial activity of oil was evaluated using agar well diffusion method. The anti-microbial test results showed that the oil had a potential anti-microbial activity against all seven Gram+ve and Gram-ve bacterial strains such as: Pseudomonas fluorescence, Salmonella typhimurium, Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Staphylococcus epidermis and Acenetobactor calcoaceticus. Essential oil showed maximum zone of inhibition and minimal inhibition concentration against Bacillus subtilis (MTCC-2451 and Pseudomonas fluorescence (MTCC-664 bacterial strains.

  16. Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram negative bacteria

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    Fohad Mabood Husain

    2015-05-01

    Full Text Available Bacterial quorum sensing (QS is a density dependent communication system that regulates the expression of certain genes including production of virulence factors in many pathogens. Bioactive plant extract/compounds inhibiting QS regulated gene expression may be a potential candidate as antipathogenic drug. In this study anti-QS activity of peppermint (Mentha piperita oil was first tested using the Chromobacterium violaceum CVO26 biosensor. Further, the findings of the present investigation revealed that peppermint oil at sub-MICs strongly interfered with acyl homoserine lactone (AHL regulated virulence factors and biofilm formation in Pseudomonas aeruginosa and Aeromonas hydrophila. The result of molecular docking analysis attributed the quorum sensing inhibitory activity exhibited by peppermint oil to menthol. Assessment of ability of menthol to interfere with quorum sensing systems of various Gram-negative pathogens comprising diverse AHL molecules revealed that it reduced the AHL dependent production of violacein, virulence factors and biofilm formation indicating broad-spectrum anti-QS activity. Using two E. coli biosensors, MG4/pKDT17 and pEAL08-2, we also confirmed that menthol inhibited both the las and pqs quorum sensing systems. Further, findings of the in vivo studies with menthol on nematode model Caenorhabditis elegans showed significantly enhanced survival of the nematode. Our data identified menthol as a novel broad spectrum quorum sensing inhibitor.

  17. Chemical Composition and Anti-Inflammatory, Cytotoxic and Antioxidant Activities of Essential Oil from Leaves of Mentha piperita Grown in China.

    Directory of Open Access Journals (Sweden)

    Zhenliang Sun

    Full Text Available The chemical composition, anti-inflammatory, cytotoxic and antioxidant activities of essential oil from leaves of Mentha piperita (MEO grown in China were investigated. Using GC-MS analysis, the chemical composition of MEO was characterized, showing that it was mainly composed of menthol, menthone and menthy acetate. MEO exhibited potent anti-inflammatory activities in a croton oil-induced mouse ear edema model. It could also effectively inhibit nitric oxide (NO and prostaglandin E2 (PGE2 production in lipopolysaccharide (LPS-activated RAW 264.7 macrophages. The cytotoxic effect was assessed against four human cancer cells. MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively. In addition, MEO had moderate antioxidant activity. The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources.

  18. [Effects of aqueous extracts of Mentha piperita L. and Chenopodium ambrosioides L. leaves in infective larvae cultures of gastrointestinal nematodes of goats].

    Science.gov (United States)

    De Almeida, Maria Angela O; Domingues, Luciana F; Almeida, Gisele N; Simas, Mônica Mattos Dos S; Botura, Mariana B; Da Cruz, Ana Carla Ferreira G; Da Silva, Ana Valéria Araújo F; Menezes, Taise P; Batatinha, Maria José M

    2007-01-01

    Phitotherapy has been frequently utilized in parasitism control for numerous animal species. The aim of this experiment was to evaluate the in vitro effects of aqueous extracts of Mentha piperita L. and Chenopodium ambrosioides L. leaves in larvae cultures of gastrointestinal nematodes of goats. Six different concentrations of M. piperita extracts (196; 150.7; 115.9; 89.1; 68.5 e 52.7 mg/mL) and C. ambrosioides extracts (110,6; 85; 65,3; 50,2; 38,6 e 29,6 mg/mL) were used for the treatment of larvae cultures, in triple assays. Distilled water and doramectin were used in larvae cultures as negative and positive controls, respectively. The results revealed a reduction of more than 95% of the infective larvae when M. piperita extracts were used in the concentrations of 115.9 and 196 mg/mL, and C. ambrosioides extract in the concentration of 110.6 mg/mL, supporting the effect of these extracts in the in vitro treatment of gastrointestinal nematodes of goats.

  19. Chemical Composition and Anti-Inflammatory, Cytotoxic and Antioxidant Activities of Essential Oil from Leaves of Mentha piperita Grown in China.

    Science.gov (United States)

    Sun, Zhenliang; Wang, Huiyan; Wang, Jing; Zhou, Lianming; Yang, Peiming

    2014-01-01

    The chemical composition, anti-inflammatory, cytotoxic and antioxidant activities of essential oil from leaves of Mentha piperita (MEO) grown in China were investigated. Using GC-MS analysis, the chemical composition of MEO was characterized, showing that it was mainly composed of menthol, menthone and menthy acetate. MEO exhibited potent anti-inflammatory activities in a croton oil-induced mouse ear edema model. It could also effectively inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. The cytotoxic effect was assessed against four human cancer cells. MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively. In addition, MEO had moderate antioxidant activity. The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources.

  20. Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria.

    Science.gov (United States)

    Husain, Fohad M; Ahmad, Iqbal; Khan, Mohammad S; Ahmad, Ejaz; Tahseen, Qudisa; Khan, Mohd Shahnawaz; Alshabib, Nasser A

    2015-01-01

    Bacterial quorum sensing (QS) is a density dependent communication system that regulates the expression of certain genes including production of virulence factors in many pathogens. Bioactive plant extract/compounds inhibiting QS regulated gene expression may be a potential candidate as antipathogenic drug. In this study anti-QS activity of peppermint (Mentha piperita) oil was first tested using the Chromobacterium violaceum CVO26 biosensor. Further, the findings of the present investigation revealed that peppermint oil (PMO) at sub-Minimum Inhibitory Concentrations (sub-MICs) strongly interfered with acyl homoserine lactone (AHL) regulated virulence factors and biofilm formation in Pseudomonas aeruginosa and Aeromonas hydrophila. The result of molecular docking analysis attributed the QS inhibitory activity exhibited by PMO to menthol. Assessment of ability of menthol to interfere with QS systems of various Gram-negative pathogens comprising diverse AHL molecules revealed that it reduced the AHL dependent production of violacein, virulence factors, and biofilm formation indicating broad-spectrum anti-QS activity. Using two Escherichia coli biosensors, MG4/pKDT17 and pEAL08-2, we also confirmed that menthol inhibited both the las and pqs QS systems. Further, findings of the in vivo studies with menthol on nematode model Caenorhabditis elegans showed significantly enhanced survival of the nematode. Our data identified menthol as a novel broad spectrum QS inhibitor.

  1. Effects of Mentha longifolia L. essential oil and nisin alone and in combination on Bacillus cereus and Bacillus subtilis in a food model and bacterial ultrastructural changes.

    Science.gov (United States)

    Pajohi, Mohamad Reza; Tajik, Hossein; Farshid, Amir Abbas; Basti, Afshin Akhondzadeh; Hadian, Mojtaba

    2011-02-01

    In the face of emerging new pathogens and ever-growing health-conscious customers, food preservation technology remains on the top agenda of food industry. This study was aimed at determining the effects of the essential oil of Mentha longifolia L., alone and in combination with nisin, on Bacillus cereus and Bacillus subtilis at 8°C and 25°C in a food model (commercial barley soup) during 15 days. The essential oil alone at 8°C inhibited bacterial growth significantly compared with the control (p oil alone showed inhibitory effect on bacterial growth. At 8°C, the combination effect of the essential oil and nisin on bacteria was noted at 0.25 μg mL(-1) for nisin and 0.05 μL mL(-1) for the essential oil (p oil demonstrated significant inhibitory effects on the vegetative forms of bacteria at 25°C, although it was comparable to that of nisin alone at the same concentrations. Electron microscopy studies revealed a great deal of damage to B. cereus treated with a combination of nisin and the essential oil. However, the combination of nisin with the essential oil led to a complete destruction of cell wall and cytoplasm of vegetative cells of B. subtilis.

  2. Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani Flora.

    Science.gov (United States)

    Iqbal, Tahseen; Hussain, Abdullah Ijaz; Chatha, Shahzad Ali Shahid; Naqvi, Syed Ali Raza; Bokhari, Tanveer Hussain

    2013-01-01

    The antioxidant activity and free radical scavenging capacity of the essential oil and three different extracts of wildly grown Mentha longifolia (M. longifolia) were studied. The essential oil from M. longifolia aerial parts was isolated by hydrodistillation technique using Clevenger-type apparatus. The extracts were prepared with three solvents of different polarity (n-hexane, dichloromethane, and methanol) using Soxhlet extractor. Maximum extract yield was obtained with methanol (12.6 g/100 g) while the minimum with dichloromethane (3.50 g/100 g). The essential oil content was found to be 1.07 g/100 g. A total of 19 constituents were identified in the M. longifolia oil using GC/MS. The main components detected were piperitenone oxide, piperitenone, germacrene D, borneol, and β -caryophyllene. The total phenolics (TP) and total flavonoids (TF) contents of the methanol extract of M. longifolia were found to be significantly higher than dichloromethane and hexane extracts. The dichloromethane and methanol extracts exhibited excellent antioxidant activity as assessed by 2,2'-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability, bleaching β -carotene, and inhibition of linoleic acid peroxidation assays. The essential oil and hexane extract showed comparatively weaker antioxidant and free radical scavenging activities. The results of the study have validated the medicinal and antioxidant potential of M. longifolia essential oil and extracts.

  3. 不同溶剂提取的薄荷浸提物的抑菌效果研究%Study on Antimicrobial Activities of Different Solvent Extracts from HERBA MENTHAE

    Institute of Scientific and Technical Information of China (English)

    刘锐; 莫倩美

    2011-01-01

    [目的]研究不同溶剂提取的薄荷(HERBA MENTHAE HAPLOCLYCIS)浸提物的抑菌效果,以期为优化薄荷提取工艺,减少2次污染,为薄荷进一步开发利用提供新途径.[方法]采用琼脂扩散法测定不同溶剂提取的薄荷浸提物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、沙门氏菌的抑菌活性.[结果]不同溶剂提取的薄荷浸提物对4种指示菌均具有的明显的抑菌作用;不同溶剂提取的薄荷浸提物的抑菌能力也不同,其中无水乙醇的薄荷浸提物的抑菌效果最好;薄荷浸提物的抑菌能力受到菌种的影响,相同的薄荷浸提物对不同菌种的抑制能力并不相同.[结论]用无水乙醇的薄荷浸提物的抑菌效果最好.%[Objective] The research aimed to study the antimicrobial activities of different solvent extrcts from HERBA MENTHAE, so as to optimize the extraction technology, decrease the second pollution and provide a new way for further exploitation and utilization of food industry.[ Method ] Agar diffusion method was performed to determine the antimicrobial activities of different solvent extracts from HERBA MENTHAE against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella. [ Result ] Different solvent extracts from HERBA MENTHAE had obvious antimicrobial activities against 4 indicator bacteria, in which absolute ethyl alcohol extracts performed the best. As for the same extract,the antimicrobial activities were varied for different strains. [ Conclusion ] Absolute ethyl alcohol extracts had the best antimicrobial activity.

  4. Comparison of chemical constituents of volatile oil from different parts of mentha haplocalyx briq%薄荷不同部位挥发油成分比较研究

    Institute of Scientific and Technical Information of China (English)

    平晟; 朱才会; 晏婷; 朱敏; 陈功; 王丽梅

    2015-01-01

    研究武汉地区野生薄荷根茎叶挥发油的成分及含量。用水蒸气蒸馏法( SD )分别提取薄荷不同部位的挥发油,然后用气相色谱-质谱法分析薄荷不同部位挥发油的化学成分。结果表明:叶片挥发油出油率是0.45%,茎挥发油出油率是0.2%,根挥发油出油率是0.02%。用气相色谱-质谱法分析三种不同部位挥发油,共鉴定出43种化合物,叶和茎挥发油主要成分是薄荷醇,根挥发油的主要成分是二十四烷。用薄荷不同部位提取的挥发油含量和成分有较大差别。为获得较高产率的薄荷醇成分,以薄荷叶获取挥发油为最佳原料。%To study the volatile components and contents of roots, stems and leaves from wild Mentha haplocalyx Briq cultivated in Wuhan.The volatile oil of roots, stems and leaves from wild Mentha haplocalyx Briq was extracted by steam distillation( SD) .The chemical components of volatile oil was analyzed by gas chromatography-mass spec-trometry (GC-MS).The yield of volatile oil from the leaf was 0.45%, The yield of volatile oil from the stem was 0. 2%, The yield of volatile oil from the roots was 0.02%.43 main vo1atile compounds were identified.Levomentho was main component of volatile oil from the leaf and the stem ,Tetracosane was main component of volatile oil from the root.There was great difference in volatile components and contents which were extracted from different part of Mentha haplocalyx Briq.In order to obtain higher yield of Levomentho, leaf of Mentha haplocalyx Briq was the best Material.

  5. Study on Antimicrobiai and Antioxidant Activity of Essential Oils and Polyphenol in Mentha Plants%薄荷属植物精油和多酚类的抑菌性及抗氧化性研究

    Institute of Scientific and Technical Information of China (English)

    吕爽; 田呈瑞; 韩莎莎

    2011-01-01

    The antimicrobial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of essential oil in Mentha piperita and Mentha cardiaca were determined in this study. Furthermore, the polyphenol content and antioxidant activity of solid residues in two mints were also investigated. The results demonstrated that the two essential oils exhibited inhibition effect on E. Coli, Staphylococcus aureus, Salmonella typhimurium and Listeria monocytogenes, however, without inhibitory effect was observed in the case of Salmonella enteritidis and Aspergillus niger. The antimicrobial ability of peppermint essential oil was stronger than Scotch spearmint essential oils. Besides, the polyphenol content in residues of peppermint was significantly decreased, but that in Scotch spearmint had no significantreduction. The residues of two mints had stronger ability of scavenging DPPH after water distillation, but the antioxidant ability in FRAP assay reduced significantly.%研究了椒样薄荷(Mentha piperita L.)与苏格兰留兰香(Mentha cardiaca G.)精油的抑菌活性,测定了精油的最小抑菌浓度(MIC)和最小杀菌浓度(MBC).同时对两种薄荷叶片以及精油提取后残渣的多酚含量和抗氧化性进行研究,以期为椒样薄荷与苏格兰留兰香的多角度开发利用提供理论依据.结果表明:2种薄荷精油对于大肠杆菌、金黄色葡萄球菌、鼠伤寒沙门氏菌和李斯特菌均有抑制作用,而对于肠炎沙门氏菌及黑曲霉没有抑制作用,椒样薄荷精油的抑菌效果比苏格兰留兰香精油强.另外,椒样薄荷多酚含量在提取精油后显著性减少,而苏格兰留兰香未显著性降低.两种薄荷精油提取后残渣具有较强的DPPH自由基清除能力,而FRAP抗氧化能力却显著性降低.

  6. Potencial alelopático de extratos aquosos de Melissa officinalis L. e Mentha x villosa L. na germinação e vigor de sementes de Plantago major L. Allelopathic effect of extracts of Melissa officinallis L. and Mentha x villosa L. on seed germination and vigor of Plantago major L.

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    F.P.G. Bonfim

    2011-01-01

    Full Text Available O objetivo do trabalho foi avaliar o efeito dos extratos aquosos de hortelã e melissa na germinação e vigor de sementes de tanchagem. O experimento foi conduzido no Departamento de Fitotecnia da Universidade Federal de Viçosa, no mês de abril de 2011. O delineamento utilizado foi o inteiramente casualizado com cinco tratamentos (testemunha, 25%, 50%, 75% e 100 % do extrato aquoso de hortelã e melissa e quatro repetições. Os extratos foram preparados utilizando a parte aérea das plantas, na proporção de 100g de planta para 1 L de água destilada. Foram utilizadas 50 sementes de tanchagem em cada caixa gerbox, previamente umedecido com os extratos aquosos (correspondentes aos tratamentos, estas foram levadas para germinar em câmara de germinação, em temperatura de 20°C, fotoperíodo de 16 horas luz e 8 horas escuro, por 14 dias. Durante esse tempo foram feitas às contagens diárias das sementes germinadas para calcular o índice de velocidade de germinação e ao final dos 14 dias foi calculada a porcentagem de germinação. Sementes de tanchagem não submetidas aos extratos aquosos de melissa e hortelã apresentaram-se mais vigorosas quando comparado com as concentrações, evidenciando o potencial alelopático de melissa e hortelã sobre a sua qualidade fisiológica.The aim of this work was to evaluate the effect of aqueous extracts of Melissa officinallis and Mentha x villosa for the germination and vigor of Plantago major seeds. The experiment was conducted at the Plant Science Department, Federal University of Viçosa, in April 2011. The design was a completely randomized design with five treatments (control, 25%, 50%, 75% and 100% of the aqueous extract of Mentha x villosa and Melissa Officinallis and four replications. The extracts were prepared utulizando the shoots of plants at the rate of 100g of plant to 1 L of distilled water. We used 50 Plantago major seeds in each box incubator and these were subsequently placed in a

  7. Evaluation of possible toxic effects of spearmint (Mentha spicata on the reproductive system, fertility and number of offspring in adult male rats

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    Fatemeh Nozhat

    2014-11-01

    Full Text Available Objective: In this study we investigated the effects of spearmint (Mentha spicata Labiatae on the reproductive system, fertility and number of offspring in adult male rats. Materials and Methods: Adult Wistar male rats in one control (C and three experimental groups (I, II and III received 0, 10, 20 and 40 mg/kg spearmint extract orally for 45 days, respectively.  Following this treatment, the animals’ weights, and the standard weight of reproductive tissues, sperm count, sperm motility and serum testosterone concentration were measured, and reproductive tissues were examined histopathologically. To evaluate the effects of spearmint on fertility of male rats and growth of their offspring, male rats of the control and experimental groups mated with untreated female rats. Results: Results showed that spearmint did not affect the rats’ body and reproductive tissue weights. The sperm count, fast and slow progressive motility of sperm and serum testosterone concentration decreased while number of non-progressive sperm and immotile sperm increased in the experimental groups compared to the control group, but none of these changes were statistically significant. Histopathological studies showed no severe changes in reproductive tissues between control and experimental groups. Number and growth of offspring born from mating of male rats with untreated female rats showed no difference. Conclusion: We concluded that spearmint has no significant toxic effect on the reproductive system, fertility and number of offspring in adult male rats at the above  mentioned dose levels. However high levels of this extract may have adverse effects on male fertility.

  8. Antioxidant activity, the content of total phenols and flavonoids in the ethanol extracts of Mentha longifolia (L. Hudson dried by the use of different techniques

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    Stanisavljević Dragana M.

    2012-01-01

    Full Text Available In this study, we have examined the yield of extracted substances obtained by means of extraction using 70 % ethanol (v/v, the content of total phenols and flavonoids, as well as the antioxidant activity of the extracts obtained from the samples of the herbs dried by means of different techniques. Wild mint Mentha longifolia (L. Hudson was dried naturally in a laboratory oven at a temperature of 45 °C and in an absorptive low temperature condensation oven at 35°C. The highest yield of extracts was obtained from the naturally dried herbs and the lowest from the herbs dried in the low temperature condensation drying oven. The content of total phenols and flavonoids was determined by spectrophotometric methods with an FC reagent and by the complexation reaction with aluminium-chloride, respectively. The extract of the naturally dried herbs had the highest overall content of phenols (113.8±2.0 mg of gallic acid/g of the dry extract and flavonoids (106.7±0.3 mg of rutin/g of the dry extract. The highest antioxidant activity determined by the FRAP and DPPH assay was determined in the extracts obtained from naturally dried herbs (2.76±0.15 mmol Fe2+/mg of the dry extract and EC50=0.022±0.001 mg/ml, while the lowest was obtained from the extracts of herbs dried in the laboratory oven (1.13±0.11 mmol Fe2+/mg of the dry extract and EC50=0.033±0.001 mg/ml. The HPLC-DAD analysis result show that the greatest content of phenolic compounds show extract obtained from naturally dried plant material. The dominant phenolic component in the all extracts is Kaempferol 3-O-glucoside. The content of all phenolic compound strongly depend on the drying conditions.

  9. Analysis of Plant Growth-Promoting Effects of Fluorescent Pseudomonas Strains Isolated from Mentha piperita Rhizosphere and Effects of Their Volatile Organic Compounds on Essential Oil Composition

    Science.gov (United States)

    Santoro, Maricel V.; Bogino, Pablo C.; Nocelli, Natalia; Cappellari, Lorena del Rosario; Giordano, Walter F.; Banchio, Erika

    2016-01-01

    Many species or strains of the genus Pseudomonas have been characterized as plant growth promoting rhizobacteria (PGPR). We used a combination of phenotypic and genotypic techniques to analyze the community of fluorescent Pseudomonas strains in the rhizosphere of commercially grown Mentha piperita (peppermint). Biochemical techniques, Amplified rDNA Restriction Analysis (ARDRA), and 16S rRNA gene sequence analysis revealed that the majority of the isolated native fluorescent strains were P. putida. Use of two Repetitive Sequence-based PCR (rep-PCR) techniques, BOX-PCR and ERIC-PCR, allowed us to evaluate diversity among the native strains and to more effectively distinguish among them. PGPR activity was tested for the native strains and reference strain P. fluorescens WCS417r. Micropropagated M. piperita plantlets were exposed to microbial volatile organic compounds (mVOCs) emitted by the bacterial strains, and plant biomass parameters and production of essential oils (EOs) were measured. mVOCs from 11 of the native strains caused an increase in shoot fresh weight. mVOCs from three native strains (SJ04, SJ25, SJ48) induced changes in M. pierita EO composition. The mVOCs caused a reduction of metabolites in the monoterpene pathway, for example menthofuran, and an increase in menthol production. Menthol production is the primary indicator of EO quality. The mVOCs produced by native strains SJ04, SJ25, SJ48, and strain WCS417r were analyzed. The obtained mVOC chromatographic profiles were unique for each of the three native strains analyzed, containing varying hydrocarbon, aromatic, and alogenic compounds. The differential effects of the strains were most likely due to the specific mixtures of mVOCs emitted by each strain, suggesting a synergistic effect occurs among the compounds present. PMID:27486441

  10. Analysis of Plant Growth-Promoting Effects of Fluorescent Pseudomonas Strains Isolated from Mentha piperita Rhizosphere and Effects of Their Volatile Organic Compounds on Essential Oil Composition.

    Science.gov (United States)

    Santoro, Maricel V; Bogino, Pablo C; Nocelli, Natalia; Cappellari, Lorena Del Rosario; Giordano, Walter F; Banchio, Erika

    2016-01-01

    Many species or strains of the genus Pseudomonas have been characterized as plant growth promoting rhizobacteria (PGPR). We used a combination of phenotypic and genotypic techniques to analyze the community of fluorescent Pseudomonas strains in the rhizosphere of commercially grown Mentha piperita (peppermint). Biochemical techniques, Amplified rDNA Restriction Analysis (ARDRA), and 16S rRNA gene sequence analysis revealed that the majority of the isolated native fluorescent strains were P. putida. Use of two Repetitive Sequence-based PCR (rep-PCR) techniques, BOX-PCR and ERIC-PCR, allowed us to evaluate diversity among the native strains and to more effectively distinguish among them. PGPR activity was tested for the native strains and reference strain P. fluorescens WCS417r. Micropropagated M. piperita plantlets were exposed to microbial volatile organic compounds (mVOCs) emitted by the bacterial strains, and plant biomass parameters and production of essential oils (EOs) were measured. mVOCs from 11 of the native strains caused an increase in shoot fresh weight. mVOCs from three native strains (SJ04, SJ25, SJ48) induced changes in M. pierita EO composition. The mVOCs caused a reduction of metabolites in the monoterpene pathway, for example menthofuran, and an increase in menthol production. Menthol production is the primary indicator of EO quality. The mVOCs produced by native strains SJ04, SJ25, SJ48, and strain WCS417r were analyzed. The obtained mVOC chromatographic profiles were unique for each of the three native strains analyzed, containing varying hydrocarbon, aromatic, and alogenic compounds. The differential effects of the strains were most likely due to the specific mixtures of mVOCs emitted by each strain, suggesting a synergistic effect occurs among the compounds present. PMID:27486441

  11. Effects of Peppermint (Mentha piperita L. Alcoholic Extract on Carbon Tetrachloride-induced Hepatotoxicity in Broiler Chickens Under Heat Stress Condition

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    Khodadust MR

    2015-06-01

    Full Text Available In order to investigate the effects of peppermint (Mentha piperita L. alcoholic extract on liver injury caused by the oxidant carbon tetrachloride (CCl4, an experiment was performed as a completely randomized design in a factorial arrangement (2 × 2 with 4 replications of 10 broilers each. Factors included two levels of peppermint leaf alcoholic extract (0 and 2 mL/Kg body weight and CCl4 (0 and 1 mL/Kg body weight. Results showed significant (P interactions for body weight gain (BWG, feed intake (FI and feed conversion ratio (FCR on d 42 of the experiment. The alcoholic extract of peppermint leaf did not improve growth performance, whereas CCl4 worsened BWG and FCR (P. The interaction between peppermint extract and CCl4 indicated an ameliorative effect of peppermint extract on BWG and FCR (P. The interaction effects between peppermint extract and CCl4 significantly differed for blood serum concentrations of total protein, albumin, albumin:globulin ratio, glucose, triglyceride, total cholesterol, HDLC, LDLC, LDLC:HDLC ratio, and VLDLC as well as the amount of blood liver enzymes (P. Peppermint extract significantly increased blood serum concentrations of total protein, albumin, triglyceride and HDLC, whilst CCl4 decreased those concentrations (P. Blood serum concentrations of total cholesterol, LDLC, LDLC:HDLC ratio, VLDLC and glucose were decreased by peppermint extract, whereas those concentrations were increased by CCl4 (P. A significantly higher level of liver enzymes was found in blood serum of birds treated by CCl4 than those by peppermint extract (P. A moderate effect on blood serum liver enzymes was observed by the interaction between 2 mL of peppermint extract and 1 mL of CCl4 (P. Generally, this study indicated that in vivo administration of peppermint alcoholic extract ameliorated the adverse effects of CCl4 on growth performance and liver function, therefore it might be useful for the prevention of oxidative stress

  12. Vascular and antioxidant effects of an aqueous Mentha cordifolia extract in experimental N(G)-nitro-L-arginine methyl ester-induced hypertension.

    Science.gov (United States)

    Pakdeechote, Poungrat; Prachaney, Parichat; Berkban, Warinee; Kukongviriyapan, Upa; Kukongviriyapan, Veerapol; Khrisanapant, Wilaiwan; Phirawatthakul, Yada

    2014-01-01

    The effect of an aqueous Mentha cordifolia (MC) extract on the haemodynamic status, vascular remodeling, function, and oxidative status in NG-nitro-L-arginine methyl ester (L-NAME)-induced hypertension was investigated. Male Sprague-Dawley rats were given L-NAME [50 mg/(kg body weight (BW) d)] in their drinking water for 5 weeks and were treated by intragastric administration with the MC extract [200 mg/(kgBWd)] for 2 consecutive weeks. Quercetin [25 mg/(kg BW d)] was used as a positive control. The effects of the MC extract on the haemodynamic status, thoracic aortic wall thickness, and oxidative stress markers were determined, and the vasorelaxant activity of the MC extract was tested in isolated mesenteric vascular beds in rats. Significant increases in the mean arterial pressure (MAP), heart rate (HR), hind limb vascular resistance (HVR), wall thickness, and cross-sectional area of the thoracic aorta, as well as oxidative stress markers were found in the L-NAME-treated group compared to the control (P < 0.05). MAP, HVR, wall thickness, cross-sectional area of the thoracic aorta, plasma malondialdehyde (MDA), and vascular superoxide anion production were significantly reduced in L-NAME hypersensitive rats treated with the MC extract or quercetin. Furthermore, the MC extract induced vasorelaxation in the pre-constricted mesenteric vascular bed with intact and denuded endothelium of normotensive and hypertensive rats. Our results suggest that the MC extract exhibits an antihypertensive effect via its antioxidant capacity, vasodilator property, and reduced vascular remodeling.

  13. In vitro inhibition of herpes simplex virus type 1 replication by Mentha suaveolens essential oil and its main component piperitenone oxide.

    Science.gov (United States)

    Civitelli, Livia; Panella, Simona; Marcocci, Maria Elena; De Petris, Alberto; Garzoli, Stefania; Pepi, Federico; Vavala, Elisabetta; Ragno, Rino; Nencioni, Lucia; Palamara, Anna Teresa; Angiolella, Letizia

    2014-05-15

    Several essential oils exert in vitro activity against bacteria and viruses and, among these latter, herpes simplex virus type 1 (HSV-1) is known to develop resistance to commonly used antiviral agents. Thus, the effects of the essential oil derived from Mentha suaveolens (EOMS) and its active principle piperitenone oxide (PEO) were tested in in vitro experimental model of infection with HSV-1. The 50% inhibitory concentration (IC50) was determined at 5.1μg/ml and 1.4μg/ml for EOMS and PEO, respectively. Australian tea tree oil (TTO) was used as control, revealing an IC50 of 13.2μg/ml. Moreover, a synergistic action against HSV-1 was observed when each oil was added in combination with acyclovir. In order to find out the mechanism of action, EOMS, PEO and TTO were added to the cells at different times during the virus life-cycle. Results obtained by yield reduction assay indicated that the antiviral activity of both compounds was principally due to an effect after viral adsorption. Indeed, no reduction of virus yield was observed when cells were treated during viral adsorption or pre-treated before viral infection. In particular, PEO exerted a strong inhibitory effect by interfering with a late step of HSV-1 life-cycle. HSV-1 infection is known to induce a pro-oxidative state with depletion of the main intracellular antioxidant glutathione and this redox change in the cell is important for viral replication. Interestingly, the treatment with PEO corrected this deficit, thus suggesting that the compound could interfere with some redox-sensitive cellular pathways exploited for viral replication. Overall our data suggest that both EOMS and PEO could be considered good candidates for novel anti-HSV-1 strategies, and need further exploration to better characterize the targets underlying their inhibition.

  14. Anti-inflammatory effect of Mentha longifolia in lipopolysaccharide-stimulated macrophages: reduction of nitric oxide production through inhibition of inducible nitric oxide synthase.

    Science.gov (United States)

    Karimian, Parastoo; Kavoosi, Gholamreza; Amirghofran, Zahra

    2013-01-01

    Mentha longifolia is an aromatic plant used in flavoring and preserving foods and as an anti-inflammatory folk medicine remedy. The present study assessed the effects of M. longifolia extracts, including essential oil and crude methanol extract and its fractions (ethyl acetate, butanol and hexane), on nitric oxide (NO) production and inducible NO synthase (iNOS) mRNA expression in lipopolysaccharide (LPS)-stimulated J774A.1 cells using real-time polymerase chain reaction (PCR). The cytotoxic effects of the extracts on the cells were examined and non-cytotoxic concentrations (<0.2 mg/ml) were used to examine their effects on NO production and iNOS mRNA expression. Only the hexane fraction that contained high levels of phenolic and flavonoid compounds at concentrations from 0.05-0.20 mg/ml significantly reduced NO production in LPS-stimulated cells (p < 0.001). Real-time PCR analysis indicated the ability of this fraction at the same concentrations to significantly decrease iNOS as well as TNFα mRNA expression in the cells (p < 0.001). All extracts were able to scavenge NO radicals in a concentration-dependent manner. At concentrations greater than 0.2 mg/ml, total radicals were 100% scavenged. In conclusion, M. longifolia possibly reduces NO secretion in macrophages by scavenging NO and inhibiting iNOS mRNA expression, and also decreases TNFα pro-inflammatory cytokine expression, thus showing its usefulness in the inflammatory disease process.

  15. Analysis of Plant Growth-Promoting Effects of Fluorescent Pseudomonas Strains Isolated from Mentha piperita Rhizosphere and Effects of Their Volatile Organic Compounds on Essential Oil Composition.

    Science.gov (United States)

    Santoro, Maricel V; Bogino, Pablo C; Nocelli, Natalia; Cappellari, Lorena Del Rosario; Giordano, Walter F; Banchio, Erika

    2016-01-01

    Many species or strains of the genus Pseudomonas have been characterized as plant growth promoting rhizobacteria (PGPR). We used a combination of phenotypic and genotypic techniques to analyze the community of fluorescent Pseudomonas strains in the rhizosphere of commercially grown Mentha piperita (peppermint). Biochemical techniques, Amplified rDNA Restriction Analysis (ARDRA), and 16S rRNA gene sequence analysis revealed that the majority of the isolated native fluorescent strains were P. putida. Use of two Repetitive Sequence-based PCR (rep-PCR) techniques, BOX-PCR and ERIC-PCR, allowed us to evaluate diversity among the native strains and to more effectively distinguish among them. PGPR activity was tested for the native strains and reference strain P. fluorescens WCS417r. Micropropagated M. piperita plantlets were exposed to microbial volatile organic compounds (mVOCs) emitted by the bacterial strains, and plant biomass parameters and production of essential oils (EOs) were measured. mVOCs from 11 of the native strains caused an increase in shoot fresh weight. mVOCs from three native strains (SJ04, SJ25, SJ48) induced changes in M. pierita EO composition. The mVOCs caused a reduction of metabolites in the monoterpene pathway, for example menthofuran, and an increase in menthol production. Menthol production is the primary indicator of EO quality. The mVOCs produced by native strains SJ04, SJ25, SJ48, and strain WCS417r were analyzed. The obtained mVOC chromatographic profiles were unique for each of the three native strains analyzed, containing varying hydrocarbon, aromatic, and alogenic compounds. The differential effects of the strains were most likely due to the specific mixtures of mVOCs emitted by each strain, suggesting a synergistic effect occurs among the compounds present.

  16. Effect of gibberellic acid and calliterpenone on plant growth attributes, trichomes, essential oil biosynthesis and pathway gene expression in differential manner in Mentha arvensis L.

    Science.gov (United States)

    Bose, Subir K; Yadav, Ritesh Kumar; Mishra, Smrati; Sangwan, Rajender S; Singh, A K; Mishra, B; Srivastava, A K; Sangwan, Neelam S

    2013-05-01

    Extensive research is going on throughout the world to find out new molecules from natural sources to be used as plant growth promoter. Mentha arvensis L. is the main source of menthol rich essential oil used commercially in various food, pharmaceutical and other preparations. Experiments were conducted on field grown plants for understanding the effect of calliterpenone (CA), a stereo-isomer of abbeokutone, in comparison to gibberellic acid (GA3) on growth attributes, trichomes, essential oil biosynthesis and expression of some oil biosynthetic pathway genes. The exogenous application of CA (1 μM, 10 μM and 100 μM) was found to be better in improving plant biomass and stolon yield, leaf area, branching and leaf stem ratio than with counterpart GA3 at the same concentrations. CA treated plants showed higher glandular trichome number, density and diameter and also correlated with enhanced oil biogenetic capacity as revealed by feeding labeled (14)C-sucrose for 72 h to excised shoots. Semi-quantitative PCR analysis of key pathway genes revealed differential up regulation under CA treatments. Transcript level of menthol dehydrogenase/menthone reductase was found highly up regulated in CA treated plants with increased content of menthone and menthol in oil. These findings demonstrate that CA positively regulated the yields by enhanced branching and higher density of trichomes resulting into higher accumulation of essential oil. The results suggest CA as a novel plant derived diterpenoid with growth promoting action and opens up new possibilities for improving the crop yields and essential oil biosynthesis in qualitative and quantitative manner.

  17. Chemical characterization and insecticidal properties of essential oils from different wild populations of Mentha suaveolens subsp. timija (Briq.) Harley from Morocco.

    Science.gov (United States)

    Kasrati, Ayoub; Alaoui Jamali, Chaima; Bekkouche, Khalid; Spooner-Hart, Robert; Leach, David; Abbad, Abdelaziz

    2015-05-01

    The present study is the first investigation of the volatile-oil variability and insecticidal properties of the endemic Moroccan mint Mentha suaveolens subsp. timija (mint timija). The yield of essential oils (EOs) obtained from different wild mint timija populations ranged from 0.20±0.02 to 1.17±0.25% (v/w). GC/MS Analysis revealed the presence of 44 oil constituents, comprising 97.3-99.9% of the total oil compositions. The main constituents were found to be menthone (1.2-62.6%), pulegone (0.8-26.6%), cis-piperitone epoxide (2.9-25.5%), piperitone (0.3-35.5%), trans-piperitone epoxide (8.1-15.7%), piperitenone (0.2-9.6%), piperitenone oxide (0.5-28.6%), (E)-caryophyllene (1.5-11.0%), germacrene D (1.0-15.7%), isomenthone (0.3-7.7%), and borneol (0.2-7.3%). Hierarchical-cluster analysis allowed the classification of the EOs of the different mint timija populations into four main groups according to the contents of their major components. This variability within the species showed to be linked to the altitude variation of the mint timija growing sites. The results of the insecticidal tests showed that all samples exhibited interesting activity against adults of Tribolium castaneum, but with different degrees. The highest toxicity was observed for the EOs belonging to Group IV, which were rich in menthone and pulegone, with LC50 and LC90 values of 19.0-23.4 and 54.9-58.0 μl/l air in the fumigation assay and LC50 and LC90 values of 0.17-0.18 and 0.40-0.52 μl/cm(2) in the contact assay.

  18. Role of substituents in cyclodextrin derivatives for enantioselective gas chromatographic separation of chiral terpenoids in the essential oils of Mentha spicata.

    Science.gov (United States)

    Pragadheesh, V S; Yadav, Anju; Chanotiya, Chandan Singh

    2015-10-01

    Enantioselective GC-FID and enantioselective GC-MS have been utilized under temperature gradient mode with differently substituted heptakis- and octakis-cyclodextrins to achieve the resolution of chiral terpenoids in the essential oil of indigenously grown cultivars of Mentha spicata. Modified cyclodextrins were derivatized in GC column for the separation of chiral terpenoids. A 2,3-diethyl-6-tert-butyldimethylsilyl-β-cyclodextrin doped into 14% cyanopropylphenyl/86%dimethylpolysiloxane (TBDE-β-CD) showed good enantioselectivity for all the studied chiral compounds excluding carvone. Carvone enantiomers were well resolved in 2,3-diacetoxy-6-tert-butyldimethylsilyl-β-cyclodextrin column (TBDA-β-CD) with enantioseparation (Es) of 1.006. A TBDE-β-CD provides maximum enantiomeric separation for β-pinene (Es 1.038), sabinene (Es 1.051), limonene (Es 1.045), isomenthone (Es 1.029) and α-terpineol (Es 1.014). Furthermore, enantiomer elution order reversal was observed for sabinene, menthone, terpinen-4-ol and menthol while changing from β- to γ-cyclodextrin phase. Carvone exhibits enantiomer elution order reversal by changing substituents i.e., methyl to acetyl at 2- & 3- position of the cyclodextrin derivative. Chiral constituents such as (+)-isomenthone, (-)-menthone, (1R,2S,5R)-(-)-menthol and (4S)-(+)-piperitone exist as a single enantiomer with >99% excess. Existence of (R)-(+)-limonene and (S)-(+)-carvone enantiomers has been proven first time in M. spicata essential oils and can be used as the marker for Indian origin.

  19. MAPK-mediated enhanced expression of vacuolar H(+)-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.).

    Science.gov (United States)

    Li, Zhe; Zhen, Zhen; Guo, Kai; Harvey, Paul; Li, Jishun; Yang, Hetong

    2016-03-01

    Vacuolar H(+)-ATPase (V-H(+)-ATPase) has been proved to be of importance in maintenance of ion homeostasis inside plant cells under NaCl stress. In this study, the expression levels and salt-tolerate function of V-H(+)-ATPase genes were investigated in the roots and leaves of a halotolerate peppermint (Mentha × piperita L.) Keyuan-1 treated with different concentrations of NaCl. Results showed that the expressions of V-H(+)-ATPase in the transcriptional, protein and activity levels were significantly enhanced in the halotolerate peppermint Keyuan-1 compared to the wild-type (WT) peppermint under 50, 100, and 150 mM NaCl treatment. Moreover, inhibition experiments exhibited that V-H(+)-ATPase activity played vital roles in the salt tolerance of peppermint Keyuan-1 to 150 mM NaCl stress through increasing the vacuolar H(+) pumping activity and Na(+) compartmentalization capacity. Furthermore, results of Western blots showed that the activity of a mitogen-activated protein kinase (MAPK) was significantly increased under different concentrations of NaCl in the halotolerate peppermint Keyuan-1, which was much higher than that of WT peppermint. Further experiments with inhibitors suggested that this MAPK protein was responsible for the enhanced expression of V-H(+)-ATPase in the halotolerate peppermint Keyuan-1. In response to NaCl stress, increase of cytoplasmic calcium concentration ([Ca(2+)]cyt) occurred upstream of MAPK activation in the halotolerate peppermint Keyuan-1. In all, these findings demonstrated that increased V-H(+)-ATPase activity was positively correlated with the enhanced salt tolerance in the halotolerate peppermint Keyuan-1, providing the theoretic basis for the further development and utilization of peppermint in saline areas.

  20. MAPK-mediated enhanced expression of vacuolar H(+)-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.).

    Science.gov (United States)

    Li, Zhe; Zhen, Zhen; Guo, Kai; Harvey, Paul; Li, Jishun; Yang, Hetong

    2016-03-01

    Vacuolar H(+)-ATPase (V-H(+)-ATPase) has been proved to be of importance in maintenance of ion homeostasis inside plant cells under NaCl stress. In this study, the expression levels and salt-tolerate function of V-H(+)-ATPase genes were investigated in the roots and leaves of a halotolerate peppermint (Mentha × piperita L.) Keyuan-1 treated with different concentrations of NaCl. Results showed that the expressions of V-H(+)-ATPase in the transcriptional, protein and activity levels were significantly enhanced in the halotolerate peppermint Keyuan-1 compared to the wild-type (WT) peppermint under 50, 100, and 150 mM NaCl treatment. Moreover, inhibition experiments exhibited that V-H(+)-ATPase activity played vital roles in the salt tolerance of peppermint Keyuan-1 to 150 mM NaCl stress through increasing the vacuolar H(+) pumping activity and Na(+) compartmentalization capacity. Furthermore, results of Western blots showed that the activity of a mitogen-activated protein kinase (MAPK) was significantly increased under different concentrations of NaCl in the halotolerate peppermint Keyuan-1, which was much higher than that of WT peppermint. Further experiments with inhibitors suggested that this MAPK protein was responsible for the enhanced expression of V-H(+)-ATPase in the halotolerate peppermint Keyuan-1. In response to NaCl stress, increase of cytoplasmic calcium concentration ([Ca(2+)]cyt) occurred upstream of MAPK activation in the halotolerate peppermint Keyuan-1. In all, these findings demonstrated that increased V-H(+)-ATPase activity was positively correlated with the enhanced salt tolerance in the halotolerate peppermint Keyuan-1, providing the theoretic basis for the further development and utilization of peppermint in saline areas. PMID:25999237

  1. Role of substituents in cyclodextrin derivatives for enantioselective gas chromatographic separation of chiral terpenoids in the essential oils of Mentha spicata.

    Science.gov (United States)

    Pragadheesh, V S; Yadav, Anju; Chanotiya, Chandan Singh

    2015-10-01

    Enantioselective GC-FID and enantioselective GC-MS have been utilized under temperature gradient mode with differently substituted heptakis- and octakis-cyclodextrins to achieve the resolution of chiral terpenoids in the essential oil of indigenously grown cultivars of Mentha spicata. Modified cyclodextrins were derivatized in GC column for the separation of chiral terpenoids. A 2,3-diethyl-6-tert-butyldimethylsilyl-β-cyclodextrin doped into 14% cyanopropylphenyl/86%dimethylpolysiloxane (TBDE-β-CD) showed good enantioselectivity for all the studied chiral compounds excluding carvone. Carvone enantiomers were well resolved in 2,3-diacetoxy-6-tert-butyldimethylsilyl-β-cyclodextrin column (TBDA-β-CD) with enantioseparation (Es) of 1.006. A TBDE-β-CD provides maximum enantiomeric separation for β-pinene (Es 1.038), sabinene (Es 1.051), limonene (Es 1.045), isomenthone (Es 1.029) and α-terpineol (Es 1.014). Furthermore, enantiomer elution order reversal was observed for sabinene, menthone, terpinen-4-ol and menthol while changing from β- to γ-cyclodextrin phase. Carvone exhibits enantiomer elution order reversal by changing substituents i.e., methyl to acetyl at 2- & 3- position of the cyclodextrin derivative. Chiral constituents such as (+)-isomenthone, (-)-menthone, (1R,2S,5R)-(-)-menthol and (4S)-(+)-piperitone exist as a single enantiomer with >99% excess. Existence of (R)-(+)-limonene and (S)-(+)-carvone enantiomers has been proven first time in M. spicata essential oils and can be used as the marker for Indian origin. PMID:26310896

  2. Metabolism of monoterpenes: early steps in the metabolism of d-neomenthyl-. beta. -D-glucoside in peppermint (Mentha piperita) rhizomes

    Energy Technology Data Exchange (ETDEWEB)

    Croteau, R.; Sood, V.K.; Renstroem, B.; Bhushan, R.

    1984-11-01

    Previous studies have shown that the monoterpene ketone l-(G-/sup 3/H) menthone is reduced to the epimeric alcohols l-menthol and d-neomenthol in leaves of flowering peppermint (Mentha piperita L.), and that a portion of the menthol is converted to methyl acetate while the bulk of the neomenthol is transformed to neomenthyl-..beta..-D-glucoside which is then transported to the rhizome. Analysis of the disposition of l-(G)/sup 3/H)menthone applied to midstem leaves of intact flowering plants allowed the kinetics of synthesis and transport of the monoterpenyl glucoside to be determined, and gave strong indication that the glucoside was subsequently metabolized in the rhizome. Studies with d-(G-/sup 3/H)neomenthyl-..beta..-D-glucoside as substrate, using excised rhizomes or rhizome segments, confirmed the hydrolysis of the glucoside as an early step in metabolism at this site, and revealed that the terpenoid moiety was further converted to a series of ether-soluble, methanol-soluble, and water-soluble products. The conversion of menthone to the lactone, and of the lactone to more polar products, were confirmed in vivo using l-(G-/sup 3/H)menthone and l-(G-/sup 3/H)-3,4-menthone lactone as substrates. Additional oxidation products were formed in vivo via the desaturation of labeled neomenthol and/or menthone, but none of these transformations appeared to lead to ring opening of the p-menthane skeleton. Each step in the main reaction sequence, from hydrolysis of neomenthyl glucoside to lactonization of menthone, was demonstrated in cell-free extracts from the rhizomes of flowering mint plants. The lactomization step is of particular significance in providing a means of cleaving the p-methane ring to afford an acyclic carbon skeleton that can be further degraded by modifications of the well-known ..beta..-oxidation sequence. 41 references, 3 figures, 1 table.

  3. Analysis of plant growth-promoting effects of fluorescent Pseudomonas strains isolated from Mentha piperita rhizosphere and effects of their volatile organic compounds on essential oil composition

    Directory of Open Access Journals (Sweden)

    Maricel Valeria Santoro

    2016-07-01

    Full Text Available Many species or strains of the genus Pseudomonas have been characterized as plant growth promoting rhizobacteria (PGPR. We used a combination of phenotypic and genotypic techniques to analyze the community of fluorescent Pseudomonas strains in the rhizosphere of commercially grown Mentha piperita (peppermint. Biochemical techniques, Amplified rDNA Restriction Analysis (ARDRA, and 16S rRNA gene sequence analysis revealed that the majority of the isolated native fluorescent strains were P. putida. Use of two Repetitive Sequence-based PCR (rep-PCR techniques, BOX-PCR and ERIC-PCR, allowed us to evaluate diversity among the native strains and to more effectively distinguish among them. PGPR activity was tested for the native strains and reference strain P. fluorescens WCS417r. Micropropagated M. piperita plantlets were exposed to microbial volatile organic compounds (mVOCs emitted by the bacterial strains, and plant biomass parameters and production of essential oils (EOs were measured. mVOCs from 11 of the native strains caused an increase in shoot fresh weight. mVOCs from three native strains (SJ04, SJ25,SJ48 induced changes in M. pierita EO composition. The mVOCs caused a reduction of metabolites in the monoterpene pathway, for example menthofuran, and an increase in menthol production. Menthol production is the primary indicator of EO quality. The mVOCs produced by native strains SJ04, SJ25,SJ48 and strain WCS417r were analyzed. The obtained mVOC chromatographic profiles were unique for each of the three native strains analyzed, containing varying hydrocarbon, aromatic, and alogenic compounds. The differential effects of the strains were most likely due to the specific mixtures of mVOCs emitted by each strain, suggesting a synergistic effect occurs among the compounds present.

  4. The Effect of Ethanol Extract of Aerial Parts of the Mentha piperita in the Acquisition, Tolerance Expression and Dependence to Morphine in Adult Male Mice

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    N Khajeh

    2015-04-01

    Full Text Available Background & aim: Morphine dependence is a compulsive pattern of drug taking, resulting from the positive reinforcement of the rewarding effects of drug taking and the negative reinforcement of withdrawal syndrome that accompanies the cessation of drug taking. The objective of this study was to investigate the effect of ethanol extract of aerial parts of the Mentha piperita in the acquisition, tolerance expression and dependence to morphine in adult male mice Methods: In the present study, 75 NMRI mice were divided into fifiteen groups. The Hot-plate test was used to survey the morphine activity. Morphine was injected (2.5, 5, 10, 20, 40 mg/kg, i.p. twice daily for seven days, except in 8th day in which morphine was administrated at a single dose (50 mg/kg. The extract (50, 75, 100 mg/kg was injected for eight days. The control animals were intact, and sham animals only received morphine. Naloxone was injected (10 mg/kg five hours after the final dose of morphine and the withdrawal signs were recorded during a 30 minute period. The data were expressed as mean values ± SEM and tested, using analysis of one-way ANOVA test. Results: Peppermint extract at doses of 75 and 100 kg significantly improved the tolerance expression and dependence to morphine in animals and significantly reduced the symptoms of withdrawal. Conclusion: Peppermint extract was commuted morphine tolerance and dependence in mice.The plant contained component(s that alleviate morphine withdrawal syndrome. The extract possibly be effective in improving tolerance to morphine.

  5. 皱叶薄荷精油的化学分类特征%Analysis on Chemotype of Volatile Oil of Mentha crispata Schrad. ex Willd

    Institute of Scientific and Technical Information of China (English)

    郭晓恒; 刘涛; 宋登敏; 雨田; 严铸云

    2014-01-01

    This study was aimed to analyze the volatile oil of Mentha crispata Schrad. ex Willd. in order to provide evidence for its chemotype and guidance for its production application. The chemical analysis was detected by headspace GC-MS. The results showed that 64 chemical compounds were detected. It was concluded that the volatile oil of M. crispata Schrad. ex Willd. mainly contained eucalyptol (35.58%), limonene (16.92%) and pinene (15.33%). It was concluded that the analysis on composition characteristics and main compounds of M. crispata Schrad. ex Willd. can provide evidences in its production application and chemotype.%目的:对皱叶薄荷精油进行成分分析,为种源鉴定提供化学分类依据并为生产应用提供指导。方法:采用顶空气相法对皱叶薄荷全草进行化学分析。结果:共得到64个化学成分,且皱叶薄荷主要由桉油素(Eucalyptol ,35.58%)、柠檬烯(Limonene ,16.92%)及蒎烯(Pinene ,15.33%)组成。结论:通过分析皱叶薄荷精油的组成特征及主要成分,可为其生产应用及种源鉴定提供化学分类佐证。

  6. Effect of harvest time and drying method on biomass production, essential oil yield, and quality of peppermint (Mentha x piperita L.).

    Science.gov (United States)

    Rohloff, Jens; Dragland, Steinar; Mordal, Ruth; Iversen, Tor-Henning

    2005-05-18

    In the period from 2000 to 2002, studies on peppermint (Mentha x piperita) herb and essential oil (EO) production have been conducted at Planteforsk, Apelsvoll Research Centre Div. Kise in Norway. The trials were aimed at finding the optimal harvest date and suitable drying methods to maximize EO yield and to obtain a desirable oil quality. Peppermint plants from the first production year (2000 and 2001) and the second production year (2002) were harvested during flowering at three developmental stages (early, full, and late bloom). Biomass and leaf production were recorded, and the water content of the plant material was detected after the application of different drying methods: instantaneous drying at 30, 50, and 70 degrees C and prewilting (ground drying) for 1 or 5 days followed by final drying at 30 degrees C. Finally, plant samples were transferred to The Plant Biocentre at NTNU, Trondheim, Norway, for hydrodistillation and gas chromatography-mass spectrometry (GC-MS) analyses of the EOs. Peppermint oil yield increased from early to full bloom and late bloom (average of all years and drying methods except for 50 and 70 degrees C: 2.95, 4.13 and 4.20 L/daa, respectively) as an effect of biomass production and leaf growth. The flavor-impact compounds, menthol and menthone, reached their optimum at full bloom (43-54 and 12-30%, respectively). Prewilting led to slight decreased EO levels after 1 day (7.7%) and 5 days of ground drying (1.5%) and no EO quality changes, compared to direct drying at 30 degrees C. The plant weight (H2O content) was drastically decreased to the average under 80 and 45% in all years, thus reducing the energy supply and costs for the necessary final drying step.

  7. In vitro study on the antimicrobial effect of hydroalcoholic extracts from Mentha arvensis L. (Lamiaceae against oral pathogens=Estudo in vitro do efeito antimicrobiano dos extratos hidroalcólicos de Mentha arvensis L. (Lamiaceae contra patógenos orais

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    Rogério Antonio Freitag

    2012-10-01

    Full Text Available In vitro tests could be a valuable tool for the evaluation of medicinal plants’ antimicrobial activity. Mentha arvensis of the Lamiaceae family is one of the most frequently traditional plants used in Brazil. Hydroalcoholic extracts of M. arvensis were analyzed for antimicrobial activity on Streptococcus mutans, Streptococcus sobrinus and Candida albicans. Three different assays (agar diffusion, broth macro- and micro-dilution methods were used to evaluate antimicrobial activity. Although hydroalcoholic extracts of M. arvensis did not show any antibacterial effect, its antifungal activity against C. albicans was revealed. According to the micro-dilution broth assay, MIC of the hydroalcoholic extract from leaves of M. arvensis on Candida albicans strains ranged between 625 and 2500 g mL-1. Results suggest that M. arvensis hydroalcoholic extract may be considered a potentially antifungal agent against C. albicans, and a possible item for human antibiotic therapy. However, further biological tests on the plant’s efficacy and side-effects are necessary before its use on humans. Testes in vitro podem ser uma ferramenta valiosa para a avaliação da atividade antimicrobiana de plantas medicinais. Mentha arvensis é uma das plantas medicinais brasileiras mais frequentemente utilizadas e pertence à família Lamiaceae. No presente estudo, extratos hidroalcólicos de M. arvensis foram analisados quanto à sua atividade antimicrobiana sobre Streptococcus mutans, Streptococcus sobrinus e Candida albicans. Três diferentes ensaios (métodos de difusão em ágar, macro e microdiluição em caldo foram utilizados para avaliação da atividade antimicrobiana. Embora os extratos hidroalcólicos de M. arvensis não demonstraram qualquer efeito antibacteriano, eles apresentaram atividade antifúngica contra C. albicans. Baseado no ensaio de microdiluição em caldo, a CIM do extrato hidroalcólico das folhas de M. arvensis sobre cepas de C. albicans

  8. Influência alelopática de hortelã (Mentha x villosa Huds. sobre emergência de plântulas de alface (Lactuca sativa L. Allelopathic influence of mint (Mentha x villosa Huds. on the emergence of lettuce (Lactuca sativa L. seedlings

    Directory of Open Access Journals (Sweden)

    J.T.L.S. Maia

    2011-01-01

    Full Text Available Objetivou-se com esse trabalho avaliar a interferência de possíveis efeitos alelopáticos de Mentha x villosa Huds. sobre a emergência de Lactuca sativa L. As atividades foram conduzidas em laboratório, no Instituto de Ciências Agrárias (ICA da Universidade Federal de Minas Gerais, em Montes Claros/MG. Foram propostos três tratamentos, sendo esses: solo A, coletado em área cultivada com hortelã no horto medicinal do ICA/UFMG; solo B, coletado em áreas adjacentes e, testemunha (papel germiteste.O delineamento experimental foi inteiramente casualizado (DIC, com sete repetições. As variáveis avaliadas foram: porcentagem e índice de velocidade de emergência. Observou-se que a emergência de plântulas de Lactuca sativa foi significativamente afetada pelo solo A (6,95% se comparada com o solo B (21,71%, representando assim, uma inibição da emergência, ou seja, efeito alelopático da hortelã sobre a emergência de alface. Já o índice de velocidade de emergência (IVE não diferiu significativamente entre os solos testados.This study aimed to assess the interference of possible allelopathic effects of Mentha x villosa Huds. on the germination of Lactuca sativa L. The activities were carried out in laboratory, at the Institute of Agricultural Sciences (ICA of the Federal University of Minas Gerais, Montes Claros, Minas Gerais State, Brazil. The following three treatments were proposed: soil A, collected in area cultivated with mint at the garden of ICA; soil B, collected in adjacent areas; and control (germitest paper. Experimental design was completely randomized (CR, with seven replicates. The evaluated variables were: germination percentage and speed index. The emergence of Lactuca sativa seedlings was significantly affected by soil A (6.95% when compared to soil B (21.71%, indicating thus emergence inhibition, i.e. an allelopathic effect of mint on lettuce emergence. On the other hand, the germination speed index (GSI did not

  9. Influência do cultivo em consórcio na produção de fitomassa e óleo essencial de manjericão (Ocimum basilicum L. e hortelã (Mentha x villosa Huds. Influence of intercropping on phytomass and essential oil production in basil (Ocimum basilicum L. and mint (Mentha x villosa Huds.

    Directory of Open Access Journals (Sweden)

    J.T.L.S. Maia

    2009-01-01

    Full Text Available As plantas medicinais produzem seus princípios ativos de acordo com os estímulos do ambiente, por isso a presença de outras plantas pode comprometer a produção dos metabólicos secundários. Assim, o presente trabalho teve como objetivo avaliar o efeito do cultivo consorciado sobre a produção de fitomassa e o teor de óleo essencial de manjericão (Ocimum basilicum L. e hortelã (Mentha x villosa Huds.. O experimento foi conduzido na área experimental do Núcleo de Ciências Agrárias da Universidade Federal de Minas Gerais, Campus Regional de Montes Claros- MG. O delineamento utilizado foi o de blocos casualizados, com doze repetições, compreendendo seis tratamentos: manjericão + cenoura, manjericão + alface, manjericão solteiro, hortelã + cenoura, hortelã + alface e hortelã solteira. A produção de fitomassa e óleo essencial da hortelã não foram influenciados pelos tratamentos. Para o manjericão, apenas a massa fresca variou significativamente com os tratamentos.Medicinal plants produce their active principles according to environmental stimuli; thus, the presence of other plants may affect the synthesis of secondary compounds. The present work aimed to evaluate the effect of intercropping on phytomass and essential oil production in basil (Ocimum basilicum L. and mint (Mentha x villosa Huds.. The study was carried out in the experimental field of the Center for Agriculture, Federal University of Minas Gerais, Montes Claros Campus, Minas Gerais State, Brazil. The experimental design was in randomized blocks, with twelve replicates and six treatments: basil + carrot, basil + lettuce, sole crop of basil, mint + carrot, mint + lettuce, and sole crop of mint. Phytomass and essential oil production in mint were not affected by the treatments. As regards basil, only fresh matter significantly varied with treatments.

  10. Control of Aspergillus flavus in maize with plant essential oils and their components.

    Science.gov (United States)

    Montes-Belmont, R; Carvajal, M

    1998-05-01

    The effects of 11 plant essential oils for maize kernel protection against Aspergillus flavus were studied. Tests were conducted to determine optimal levels of dosages for maize protection, effects of combinations of essential oils, and residual effects and toxicity of essential oils to maize plants. Principal constituents of eight essential oils were tested for ability to protect maize kernels. Essential oils of Cinnamomum zeylanicum (cinnamon), Mentha piperita (peppermint), Ocimum basilicum (basil), Origanum vulgare (origanum), Teloxys ambrosioides (the flavoring herb epazote), Syzygium aromaticum (clove), and Thymus vulgaris (thyme) caused a total inhibition of fungal development on maize kernels. Thymol and o-methoxycinnamaldehyde significantly reduced maize grain contamination. The optimal dosage for protection of maize varied from 3 to 8%. Combinations of C. zeylanicum with the remaining oils gave efficient control. A residual effect of C. zeylanicum was detected after 4 weeks of kernel treatment. No phytotoxic effect on germination and corn growth was detected with any of these oils.

  11. 气相色谱法测定薄荷药材及饮片中的薄荷脑%Determination of menthol in menthae haplocalycis herba and its slices by GC

    Institute of Scientific and Technical Information of China (English)

    胡青; 张甦; 王柯; 季申

    2011-01-01

    Objective:To establish a GC method to determine the menthol in Menthae Haplocalycis Herba and its slices. Methods: The menthol was determined following a temperature change programme with INNOWAX capillary column(30 m × 0.53 mm i. d. × 1 μm film thicknes). The testing solution was prepared in ultrasonic extraction with n - hexane. Results: The linear relationship of menthol was good in the range of 9.36 ng -4680 ng. The average recovery was 97.8%. Conclusion: The methods were available with good reproducibility , and could control the quality of Menthae Haplocalycis Herba and its slices effectively.%目的:建立薄荷药材及饮片中薄荷脑的气相色谱含量测定方法,控制其质量.方法:采用INNOWAX弹性石英毛细管柱(柱长30 m,柱内径0.53 mm,膜厚1 μm);程序升温.供试品溶液的制备采用正己烷超声提取.结果:薄荷脑在9.36 ng~4680 ng范围内线性关系良好,平均回收率为97.8%.结论:该方法可行,重复性好,可用于控制薄荷药材与饮片的质量.

  12. Studies on Chemical Constituents and Antimicrobial Activity of Mentha arvensis Oil of Yunnan%云南薄荷精油的化学成分及其抗菌活性研究

    Institute of Scientific and Technical Information of China (English)

    周露; 谢文申

    2011-01-01

    采用GC/MS法对云南薄荷精油挥发性成分进行鉴定,检测出49个成分,鉴定了其中44个成分.薄荷精油主要化学成分为薄荷醇、薄荷酮、苧烯、1,8-桉叶素、胡椒酮、大根香叶烯D、乙酸薄荷酯等.试验研究了薄荷精油对大肠杆菌、金黄葡萄球菌、烟曲霉菌、白念珠菌的抗菌活性.结果显示精油对大肠杆菌、金黄葡萄球菌、白念珠菌有明显的抗菌活性.%The chemical constituents of the Mentha arvensis oil of Yunnan were studied by GC/MS method in this paper. 49 Constituents were isolated, 44 constituents were identified. They are menthol, menthone, limonene, 1,8-cineole, piperitone, germacrene-D and menthyl acetate, etc. The study on the antimicrobial activity of the Mentha arvensis essential oil against Staphylococcus aureus, Escherichia coli, Aspergillus fumigatus and Candida albicans were also conducted. The results show that the oil has antimicrobial activity to Escherichia coli, Staphylococcus aureus and Candida albicans.

  13. Alternativas de propagação na produção de óleo essencial de Mentha canadensis L. no Litoral Norte Catarinense Propagation alternatives for the production of Mentha canadensis L. essential oil at the Santa Catarina State northern coast

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    V.M.C.S. Santos

    2012-01-01

    Full Text Available O gênero Mentha é cultivado mundialmente para a produção de óleo essencial, com ênfase no constituinte mentol, amplamente utilizado nas indústrias farmacêutica, cosmética, alimentícia e de higiene pessoal. Este trabalho teve como objetivo avaliar o efeito da utilização de diferentes estruturas de propagação e épocas de colheita de Mentha canadensis L. no Litoral Norte Catarinense. O delineamento experimental foi em blocos ao acaso em esquema fatorial 4x2, comparando quatro estruturas de propagação (estolões com 10, 15 e 20 cm de comprimento e estacas com 5 cm de comprimento e duas épocas de colheita (95 dias após o plantio e 60 dias após a rebrota. Em cada colheita foi avaliado o crescimento vegetativo e produtividade de óleo essencial e de mentol. Na primeira colheita, houve maior acúmulo de massa seca de folhas, caules e total; produtividade de óleo essencial e de mentol quando o plantio foi realizado com mudas obtidas por estaquia. Na segunda colheita, no entanto, não houve diferença significativa entre os tratamentos para todas as variáveis analisadas. A primeira colheita resultou em médias superiores em relação à segunda colheita à exceção do plantio por estolão com 10 cm em todas as variáveis e estolão com 20 cm para a massa seca de caules. Os constituintes majoritários do óleo essencial foram mentol, mentona e neomentol. Os teores de mentol foram significativamente superiores na segunda colheita e de mentona na primeira colheita, sendo que o neomentol não apresentou diferença significativa em ambas as colheitas. Os resultados obtidos sugerem que a colheita antecipada da rebrota proporciona maior concentração de mentol no óleo essencial. O uso de estolões como estrutura de propagação pode ser considerada uma alternativa viável para menta, pois além de apresentar níveis similares de produção de óleo essencial e de mentol a partir da segunda colheita ao de áreas implantadas com mudas

  14. СРАВНИТЕЛЬНЫЙ АНАЛИЗ КОМПОНЕНТНОГО СОСТАВА ЭФИРНОГО МАСЛА MENTHA PIPERITA L. ИЗ ДИКОРАСТУЩИХ ПОПУЛЯЦИЙ МОЛДОВЫ

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    Elena PELEAH

    2015-12-01

    Full Text Available ANALIZA COMPARATIVĂ A ELEMENTELOR CONSTITUTIVE ALE ULEIULUI ETERIC MENTHA PIPERITA L. DIN POPULAŢIILE SPONTANE DIN MOLDOVAÎn lucrare sunt prezentate rezultatele diferitelor forme spontane ale speciei de mentă M.piperita L. din Moldova. Populaţiile studiate de mentă diferă în ce priveşte ponderea de ulei eteric. În compoziţia chimică a uleiurilor eterice se conţin substanţe din grupa de mentolă în diferite proporţii.COMPARATIVE ANALYSIS OF THE COMPONENT COMPOSITION OF ESSENTIAL OIL OF MENTHA PIPERITA L. FROM WILD POPULATIONS OF MOLDOVAIn course of our investigation of genus Mentha we have analyzed the essential oils of some widely growing population of Mentha piperita L. collected in different regions of Moldova. The essential oils showed the same chemical composition but oil compounds in various proportions.

  15. Antimicrobial activity of propolis and essential oils and synergism between these natural products

    OpenAIRE

    IS Probst; JM Sforcin; VLM Rall; AAH Fernandes; A Fernandes Júnior

    2011-01-01

    In the present study, Apis mellifera propolis and essential oils (EOs) obtained from aromatic plants were evaluated as alternative antimicrobials. We aimed to establish the antimicrobial activity of ethanolic extracts of propolis (EEP) from Apis mellifera and of EOs from Caryophyllus aromaticus, Zingiber officinale, Cinnamomum zeylanicum and Mentha piperita against 32 Staphylococcus aureus and Escherichia coli strains from human clinical specimens. The antimicrobials were diluted in agar and ...

  16. Activities of Ten Essential Oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 Cancer Cells

    OpenAIRE

    Yuangang Zu; Huimin Yu; Lu Liang; Yujie Fu; Thomas Efferth; Xia Liu; Nan Wu

    2010-01-01

    Ten essential oils, namely, mint (Mentha spicata L.,Lamiaceae), ginger (Zingiber officinaleRosc.,Zingiberaceae), lemon (Citrus limon Burm.f.,Rutaceae), grapefruit (Citrus paradisi Macf., Rutaceae), jasmine (Jasminum grandiflora L.,Oleaceae), lavender (Mill.,Lamiaceae), chamomile (Matricaria chamomilla L., Compositae), thyme (Thymus vulgaris L., Lamiaceae), rose (Rosa damascena Mill.,Rosaceae) and cinnamon (Cinnamomum zeylanicumN. Lauraceae) were tested for their antibacterial activities towar...

  17. 薄荷不同组分对小鼠急性毒性实验比较研究%Experimental Comparis on Study on Mice's Acute Toxicity of Different Composition in Herba Menthae

    Institute of Scientific and Technical Information of China (English)

    李晓宇; 孙蓉

    2012-01-01

    ObJGCtive To compare the acute toxicity of different components in Herba Menthae on mice which is all components, peppermint oil, water extract, make an observation and evaluation of acute toxicity to Herba Menthae in order to provide a basis for safe and reasonable medication in clinical. Methods Respectively prepare the all components, peppermint oil, water extract, and compare the acute toxicity of different components in Herba Menthae on mice in accordance with classical acute toxicity test methods, and the experimental datas are calculated LD50 with the Bliss method, with the continuous administration and observation for 14 days. Results The LD^ of peppermint oil is 1.4895 ml'kg'd"1 and the 95%confidence interval is 1.3508^.6407 mL"kg-1"d~~1; the water extract in Herba Menthae are unable to make LD50, MTD results calculated in accordance with crude drug content were respectively 64.0g'kg~1-d~1, which is equal to 746.7 times of 70kg people's daily dried medicinal herb expenses, the MLD of all component is 24.36 g'kg^'d"1, which is equal to 284.2 times of 70kg people's daily dried medicinal herb expenses. Conclusion The acute toxicity of different components in Herba Menthae on mice is: peppermint oil> water extract> all components. By comparing the acute toxicity of different components in Herba Menthae on mice, it is beneficial to make an observation and evaluation of acute toxicity to Herba Menthae herbs, provide experimental basis for further toxicity-based parts and toxical substances and provide a basis for safe and reasonable medication in clinical.%目的 比较薄荷全组分、水提组分、挥发油对小鼠的急性毒性作用 .方法 分别制备薄荷全组分、水提组分和挥发油,按照经典小鼠急性毒性试验方法,进行薄荷不同组分的小鼠急性毒性试验比较研究,试验数据用公斤体重法计算半数致死量(LD50)、最大耐受量(MTD)和最大给药量(MLD),连续观察14 d,记录小

  18. The Study of the Chemical Composition of Essential Oils Mentha Piperita L. are Grown in Non-Chernozem Zone of Russia

    Directory of Open Access Journals (Sweden)

    L. SUSHKOVA

    2014-07-01

    Full Text Available By gas-liquid chromatography and mass spectrometry has been investigated the composition of essential oils and the change in the ontogenesis and exogenous effects on the plant Mentha piperita L. drugs retardant type. With the introduction of oil crops from the southern regions to the more northern regions of the component composition of the essential oil practically does not change. There are only minor variations in the content of some terpenoids oils. Exogenous preharvest treatment plant growth regulators can in some cases deliberately influence the activity of various terpenoid biosynthesis and increase the content of the most valuable components of the essential oil.Content and composition of EM is largely determined by factors such as age and leaves of plants, as well as different climatic cal, soil and agronomic conditions. Processing plants different phytoregulators also affects the content and composition of oil. Biosynthesis of terpenoids polyenzyme performed in centers, the activity and the nature of which is determined primarily genetic characteristics of plants, in addition, the activity of certain enzymes of these centers is under the control of hormonal balance and changes in ontogeny, as well as under the influence of exogenous factors.It is shown that the formation of the maximum bioefficiency in ontogeny of aromatic plants, it is advisable to use a two-stage technology to grow them. Plants producing terpenoids of essential oils, the initial step is to create the conditions for the formation of the maximum yield of aboveground mass of plants, including the use of synthetic plant growth regulators. In the second stage "biosynthetic" upon the occurrence of the reproductive phase before harvesting plants, we recommend that inhibit the growth of phytoregulators retardant type. In this case, we observe stimulation accumulation in aboveground mass of secondary metabolites. Inhibition of growth in the preharvest period, aromatic plants

  19. Effect of mint (Mentha piperita L. and caraway (Carum carvi L. on the growth of some toxigenic aspergillus species and aflatoxin B1 production

    Directory of Open Access Journals (Sweden)

    Škrinjar Marija M.

    2009-01-01

    Full Text Available An inhibitory effect of various concentrations (0.0, 0.5, 1.0, 1.5 and 2,0% of mint (Mentha piperita L. and caraway (Carvum carvi L. on the growth of A. fumigatus, A. flavus and A. ochraceus was examined during 10 days of cultivation in YES medium at temperature of 25°C. Mint showed stronger inhibitory effect than caraway. Total dry weight (g/l after 10 days of the growth of A. fumigatus in YES medium with 0.5% of mint decreased by about 95%, A. flavus by 97% and A. ochraceus by about 82%. Addition of higher concentrations of mint (1.0, 1.5 and 2.0% reduced the growth of all tested species. It was poor and hardly visible. pH values of the media increased with the increase of mint concentrations. A. fumigatus showed the highest sensitivity towards caraway and A. flavus the lowest. Total dry weight (g/l after 10 days of growth of A. fumigatus in medium with 0.5% of caraway decreased by about 72% in comparison to the control. In media with higher concentrations of caraway, its growth was found to be very poor. Concentration of 1.0% of caraway reduced A. flavus growth by 15% and of 1.5% by 92%, in regard to the control. In medium with 2.0% of caraway the growth of A. flavus was observed as poor and hardly visible. The growth of A. ochraceus in medium with 0.5% of caraway decreased by about 85% comparing with control and further decrease was noticed by the increase of concentrations. In medium with 1.5% of caraway a reduction of about 95% of growth was found and under 2.0% of caraway it was poor. pH of the media also increased with the increase of caraway concentrations. Applied concentrations of mint and caraway inhibited completely the production of AB1 by A. flavus.

  20. Effect of Mentha pulegium extract and 8-hydroxy quinoline sulphate to extend the quality and vase life of rose (Rosa hybrid) cut flower.

    Science.gov (United States)

    Hashemabadi, Davood; Torkashvand, Ali Mohammadi; Kaviani, Behzad; Bagherzadeh, Maryam; Rezaalipour, Mohaddeseh; Zarchini, Mohammad

    2015-01-01

    Rose is an ornamental plant which contains one of the world's top cut flowers. Vase life of cut rose flower is short. Extracts of Mentha pulegium and 8-hydroxy quinoline sulphate (8-HQS) were used as two preservative solutions, aiming to extend the vase life of cut rose (Rosa hybrid L.) flowers. Rose flowers were treated with a vase solution containing the extract of M. pulegium, at concentrations of 0, 10, 20 and 30%, in combination with 8-HQS at concentrations of 0, 200, 400 and 600 mg l(-1). Longevity of cut roses flowers was determined on the basis of wilting and chlorophyll retention. Cut roses flowers were kept at room temperature (20 ± 2 degrees C) under normal day light and natural ventilation. The vase life of cut flowers studied was prolonged by all 8-HQS and extract treatments. The best concentration of 8-HQS and extractwere 400 mg l(-1) and 10%, respectively. Our results indicated that the flowers treated with the extract and 8-HQS had longer vase life, higher rate of solution uptake and lower SPAD value (total chlorophyll) compared to the control. Also, cut flowers treated with the extract and 8-HQS had least bacterial colonies. The greatest longevity of vase life by 11.20 and 10.25 days was related to 400 mg I(-1) 8-HQS and 10% of extract, respectively. These treatments improved cut vase life more than the control treatment. The maximum solution uptake (1.85 ml g(-1) f.wt.) and minimum SPAD value (2.19) were calculated in 30% extract along with 200 mg l(-1) 8-HQS, and 200 mg l(-1) 8-HQS, respectively. The lowest number of bacterial colonies (55.75) was obtained in treatment of 600 mg l(-1) 8-HQS. Flower quality of specimens treated with extract and 8-HQS was better than those of the control. The experiments were repeated three times with three replicates and a completely randomized design had been used. The present study concludes that it would be possible to use preservative solutions containing extract of M. pulegium L. and 8-HQS to extend vase