Database Description - GETDB | LSDB Archive [Life Science Database Archive metadata

Lifescience Database Archive (English)

Full Text Available abase Description General information of database Database name GETDB Alternative n...ame Gal4 Enhancer Trap Insertion Database DOI 10.18908/lsdba.nbdc00236-000 Creator Creator Name: Shigeo Haya... Chuo-ku, Kobe 650-0047 Tel: +81-78-306-3185 FAX: +81-78-306-3183 E-mail: Database classification Expression... Invertebrate genome database Organism Taxonomy Name: Drosophila melanogaster Taxonomy ID: 7227 Database des...riginal website information Database maintenance site Drosophila Genetic Resource

Regulation of homologous recombination repair protein Rad51 by Ku70

International Nuclear Information System (INIS)

Du Liqing; Liu Qiang; Wang Yan; Xu Chang; Cao Jia; Fu Yue; Chen Fenghua; Fan Feiyue

2013-01-01

Objective: To explore the regulative effect of non-homologous end joining (NHEJ)protein Ku70 on homologous recombination repair protein Rad51, and to investigate the synergistic mechanism of homologous recombination repair in combination with NHEJ. Methods: Observed Rad51 protein expression after transfect Ku70 small interfering RNA or Ku70 plasmid DNA into tumor cells using Western blot. Results: Expression of Rad51 was obviously reduced after pretreated with Ku70 small interfering RNA. And with the increasing expression of Ku70 protein after transfection of Ku70 plasmid DNA PGCsi3.0-hKu70 into tumor cell lines, the Rad51 protein expression was increased. Conclusion: Ku70 protein has regulating effect on gene expression of Rad51, and it might participate in the collaboration between homologous recombination repair and NHEJ. (authors)

Gode råd til KU om bæredygtig omstilling

DEFF Research Database (Denmark)

Lund, Jens Friis

2016-01-01

I gennemsnit flyver alle KU-ansatte retur til Cairo årligt. KU's stigende internationalisering skader klimaet, og det kræver bæredygtig omstilling.......I gennemsnit flyver alle KU-ansatte retur til Cairo årligt. KU's stigende internationalisering skader klimaet, og det kræver bæredygtig omstilling....

Interaction of caveolin-1 with Ku70 inhibits Bax-mediated apoptosis.

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Huafei Zou

Full Text Available Caveolin-1, the structural protein component of caveolae, acts as a scaffolding protein that functionally regulates signaling molecules. We show that knockdown of caveolin-1 protein expression enhances chemotherapeutic drug-induced apoptosis and inhibits long-term survival of colon cancer cells. In vitro studies demonstrate that caveolin-1 is a novel Ku70-binding protein, as shown by the binding of the scaffolding domain of caveolin-1 (amino acids 82-101 to the caveolin-binding domain (CBD of Ku70 (amino acids 471-478. Cell culture data show that caveolin-1 binds Ku70 after treatment with chemotherapeutic drugs. Mechanistically, we found that binding of caveolin-1 to Ku70 inhibits the chemotherapeutic drug-induced release of Bax from Ku70, activation of Bax, translocation of Bax to mitochondria and apoptosis. Potentiation of apoptosis by knockdown of caveolin-1 protein expression is greatly reduced in the absence of Bax expression. Finally, we found that overexpression of wild type Ku70, but not a mutant form of Ku70 that cannot bind to caveolin-1 (Ku70 Φ→A, limits the chemotherapeutic drug-induced Ku70/Bax dissociation and apoptosis. Thus, caveolin-1 acts as an anti-apoptotic protein in colon cancer cells by binding to Ku70 and inhibiting Bax-dependent cell death.

Ku-pa-ro en las tablillas de Cnoso

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José L. Melena

1974-12-01

Full Text Available This paper is intended to be a comprehensive analysis of the evidence of ku-pa-ro in the Knossos Linear B tablets. Its main purpose is to set up the importance of such aromatic plant in the Mycenaean economy at Knossos and to identify what kind of sedge was concealed under the name ku-pa-ro and what part of the plant was used and for what purposes. What it is stressed is the usage of the Cyperus rotundus L. (i. e. the ku-pa-ro in the industry of perfumes and as a food additive as well. What is inferred from the discussion on the evidence of ku-pa-ro issues a series of valuable data concerning the localization of certain place-names in the map of Crete, and concerning the explanation of ideogram *171. The high qualities of ku-pa-ro preserved in the tablets lead the author to assume that such a plant was cultivated in Crete and was one of the main aromatic plants used by the Mycenaeans in the making of perfumes.

Ku70 inhibits gemcitabine-induced DNA damage and pancreatic cancer cell apoptosis

International Nuclear Information System (INIS)

Ma, Jiali; Hui, Pingping; Meng, Wenying; Wang, Na; Xiang, Shihao

2017-01-01

The current study focused on the role of Ku70, a DNA-dependent protein kinase (DNA-PK) complex protein, in pancreatic cancer cell resistance to gemcitabine. In both established cell lines (Mia-PaCa-2 and PANC-1) and primary human pancreatic cancer cells, shRNA/siRNA-mediated knockdown of Ku70 significantly sensitized gemcitabine-induced cell death and proliferation inhibition. Meanwhile, gemcitabine-induced DNA damage and subsequent pancreatic cancer cell apoptosis were also potentiated with Ku70 knockdown. On the other hand, exogenous overexpression of Ku70 in Mia-PaCa-2 cells suppressed gemcitabine-induced DNA damage and subsequent cell apoptosis. In a severe combined immune deficient (SCID) mice Mia-PaCa-2 xenograft model, gemcitabine-induced anti-tumor activity was remarkably pontificated when combined with Ku70 shRNA knockdown in the xenografts. The results of this preclinical study imply that Ku70 might be a primary resistance factor of gemcitabine, and Ku70 silence could significantly chemo-sensitize gemcitabine in pancreatic cancer cells. - Highlights: • Ku70 knockdown sensitizes gemcitabine-induced killing of pancreatic cancer cells. • Ku70 knockdown facilitates gemcitabine-induced DNA damage and cell apoptosis. • Ku70 overexpression deceases gemcitabine's sensitivity in pancreatic cancer cells. • Ku70 knockdown sensitizes gemcitabine-induced anti-tumor activity in vivo.

Ku70, Ku80, and sClusterin: A Cluster of Predicting Factors for Response to Neoadjuvant Chemoradiation Therapy in Patients With Locally Advanced Rectal Cancer

International Nuclear Information System (INIS)

Pucci, Sabina; Polidoro, Chiara; Joubert, Alessandro; Mastrangeli, Francesca; Tolu, Barbara; Benassi, Michaela; Fiaschetti, Valeria; Greco, Laura; Miceli, Roberto; Floris, Roberto; Novelli, Giuseppe; Orlandi, Augusto; Santoni, Riccardo

2017-01-01

Purpose: The identification of predictive biomarkers for neoadjuvant chemoradiation therapy (CRT) is a current clinical need. The heterodimer Ku70/80 plays a critical role in DNA repair and cell death induction after damage. The aberrant expression and localization of these proteins fail to control DNA repair and apoptosis. sClusterin is the Ku70 partner that sterically inhibits Bax-dependent cell death after damage in some pathologic conditions. This study sought to evaluate the molecular relevance of Ku70-Ku80-Clu as a molecular cluster predicting the response to neoadjuvant CRT in patients with locally advanced rectal cancer (LARC). Methods and Materials: Patients enrolled in this study underwent preoperative CRT followed by surgical excision. A retrospective study based on individual response, evaluated by computed tomography and diffusion-weighted magnetic resonance imaging, identified responder (56%) and no-responder patients (44%). Ku70/80 and Clu expression were observed in biopsy specimens obtained before and after treatment with neoadjuvant CRT from the same LARC patients. In vitro studies before and after irradiation were also performed on radioresistant (SW480) and radiosensitive (SW620) colorectal cancer cell lines, mimicking sensitive or resistant tumor behavior. Results: We found a conventional nuclear localization of Ku70/80 in pretherapeutic tumor biopsies of responder patients, in agreement with their role in DNA repair and regulating apoptosis. By contrast, in the no-responder population we observed an unconventional overexpression of Ku70 in the cytoplasm (P<.001). In this context we also overexpression of sClu in the cytoplasm, which accorded with its role in stabilizing of Bax-Ku70 complex, inhibiting Bax-dependent apoptosis. Strikingly, Ku80 in these tumor tissues was lost (P<.005). In vitro testing of colon cancer cells finally confirmed the results observed in tumor biopsy specimens, proving that Ku70/80-Clu deregulation is extensively

Ku70, Ku80, and sClusterin: A Cluster of Predicting Factors for Response to Neoadjuvant Chemoradiation Therapy in Patients With Locally Advanced Rectal Cancer

Energy Technology Data Exchange (ETDEWEB)

Pucci, Sabina, E-mail: sabina.pucci@uniroma2.it; Polidoro, Chiara; Joubert, Alessandro; Mastrangeli, Francesca; Tolu, Barbara; Benassi, Michaela; Fiaschetti, Valeria; Greco, Laura; Miceli, Roberto; Floris, Roberto; Novelli, Giuseppe; Orlandi, Augusto; Santoni, Riccardo

2017-02-01

Purpose: The identification of predictive biomarkers for neoadjuvant chemoradiation therapy (CRT) is a current clinical need. The heterodimer Ku70/80 plays a critical role in DNA repair and cell death induction after damage. The aberrant expression and localization of these proteins fail to control DNA repair and apoptosis. sClusterin is the Ku70 partner that sterically inhibits Bax-dependent cell death after damage in some pathologic conditions. This study sought to evaluate the molecular relevance of Ku70-Ku80-Clu as a molecular cluster predicting the response to neoadjuvant CRT in patients with locally advanced rectal cancer (LARC). Methods and Materials: Patients enrolled in this study underwent preoperative CRT followed by surgical excision. A retrospective study based on individual response, evaluated by computed tomography and diffusion-weighted magnetic resonance imaging, identified responder (56%) and no-responder patients (44%). Ku70/80 and Clu expression were observed in biopsy specimens obtained before and after treatment with neoadjuvant CRT from the same LARC patients. In vitro studies before and after irradiation were also performed on radioresistant (SW480) and radiosensitive (SW620) colorectal cancer cell lines, mimicking sensitive or resistant tumor behavior. Results: We found a conventional nuclear localization of Ku70/80 in pretherapeutic tumor biopsies of responder patients, in agreement with their role in DNA repair and regulating apoptosis. By contrast, in the no-responder population we observed an unconventional overexpression of Ku70 in the cytoplasm (P<.001). In this context we also overexpression of sClu in the cytoplasm, which accorded with its role in stabilizing of Bax-Ku70 complex, inhibiting Bax-dependent apoptosis. Strikingly, Ku80 in these tumor tissues was lost (P<.005). In vitro testing of colon cancer cells finally confirmed the results observed in tumor biopsy specimens, proving that Ku70/80-Clu deregulation is extensively

Discovery and characterization of Ku acetylation in Mycobacterium smegmatis.

Science.gov (United States)

Zhou, Ying; Chen, Tao; Zhou, Lin; Fleming, Joy; Deng, Jiaoyu; Wang, Xude; Wang, Liwei; Wang, Yingying; Zhang, Xiaoli; Wei, Wenjing; Bi, Lijun

2015-03-01

Lysine acetylation is an important post-translational modification and is known to regulate many eukaryotic cellular processes. Little, however, is known about acetylated proteins in prokaryotes. Here, using immunoblotting, mass spectrometry and mutagenesis studies, we investigate the acetylation dynamics of the DNA repair protein Ku and its relationship with the deacetylase protein Sir2 and the non-homologous end joining (NHEJ) pathway in Mycobacterium smegmatis. We report that acetylation of Ku increases with growth, while NHEJ activity decreases, providing support for the hypothesis that acetylation of Ku may be involved in the DNA damage response in bacteria. Ku has multiple lysine sites. Our results indicate that K29 is an important acetylation site and that deficiency of Sir2 or mutation of K29 affects the quantity of Ku and its acetylation dynamics. Our findings expand knowledge of acetylation targets in prokaryotes and indicate a new direction for further research on bacterial DNA repair mechanisms. © FEMS 2014. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Interspecific variation of intracellular localization and postirradiation movement of Ku70-protein in fibroblastic cells

International Nuclear Information System (INIS)

Endoh, Daiji; Hayashi, Masanobu; Okui, Toyo; Kawase, Shiro; Kon, Yasushiro

2003-01-01

Ku (Ku70 and Ku80) Proteins are known as components of DNA-dependent protein kinase (DNA-PK) and play an important role for DNA repair. We previously reported that more than 70% of Ku proteins were located in cytoplasm of rat cells, the Ku proteins moved into nuclei of normal rat cells after X-irradiation, Ku proteins also moved into nuclei after X-irradiation but were not retained in nucleus of radiosensitive LEC rat cells. While reports have been shown about mechanisms on nuclear localization of Ku proteins, how Ku proteins export from nucleus is poorly understood. Here we show that C-terminal region of Ku70 protein is important for its cytoplasmic localization. When transfected into LEC rat cells, exogenous intact Ku70 (1-609) tagged with enhanced green fluorescent protein (EGFP-Ku70) localized mainly in the cytoplasm, whereas C-terminal-deletion mutant of Ku70 (1-593) tagged with EGFP (EGFP-Ku70D) was mainly localized in the nucleus. After X-irradiation, the endogenous intact EGFP-Ku70 once moved into nucleus, but returned into the cytoplasm. On the other hand, EGFP-Ku70D was retained in nucleus for two hours after X-irradiation. These results suggest that C-terminal region of Ku70 is included in the postirradiation nuclear export. Next, we investigated the intracellular localization of Ku70 proteins and the movement after X-irradiation of fibroblastic cells prepared from some mammalian species. Ku70 proteins were localized in nucleus and the postirradiation-extranuclear transport was not observed in human and African green monkey cells. On the other hand, Ku70 proteins were mainly localized in cytoplasm and moved into nucleus in mouse, Chinese hamster, Golden hamster, cotton rat, squirrel, cat and dog cells. These results may show that alternatively Ku70 protein is localized in the cytoplasm or nucleus depends on species and translocation of cytoplasmic Ku70 into nucleus is a response against low dose irradiation in fibroblasts of rodents, cats and dogs

Expression of Ku correlates with radiation sensitivities in the head and neck cancer cell lines

International Nuclear Information System (INIS)

Lee, Sang Wook; Yu, Eun Sil; Yi, So Lyoung; Son, Se Hee; Kim, Jong Hoon; Ahn, Seung Do; Shin, Seong Soo; Choi, Eun Kyung

2004-01-01

DNA-dependent protein kinase (DNA-PK) is a serine/threonine kinase consisting of a 470 kDa catalytic subunit (DNA-PKcs) and a heterodimeric regulatory complex, called Ku, which is composed of 70 kDa (Ku 70) and 86 kDa (Ku 80) proteins. The DNA-PK has been shown to play a pivotal role in rejoining DNA double-strand-breaks (dsb) in mammalian cells. The purpose of this study is to examine the relationship between the level of Ku expression and radiation sensitivity. Nine head and neck, cancer cell lines showed various intrinsic radiation sensitivities. Among the nine, AMC-HN-3 cell was the most sensitive for X-ray irradiation and AMC-HN-9 cell was the most resistance. The most sensitive and resistant cell lines were selected and the test sensitivity of radiation and expression of Ku were measured. Radiation sensitivity was obtained by colony forming assay and Ku protein expression using Western blot analysis. Ku80 increased expression by radiation, wheras Ku70 did not. Overexpression of Ku80 protein increased radiation resistance in AMC-HN9 cell line. There was a correlation between Ku80 expression and radiation resistance. Ku80 was shown to play an important role in radiation damage response. Induction of Ku80 expression had an important role in DNA damage repair by radiation. Ku80 expression may be an effective predictive assay of radiosensitivity on head and neck cancer

Ku must load directly onto the chromosome end in order to mediate its telomeric functions.

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Christopher R Lopez

2011-08-01

Full Text Available The Ku heterodimer associates with the Saccharomyces cerevisiae telomere, where it impacts several aspects of telomere structure and function. Although Ku avidly binds DNA ends via a preformed channel, its ability to associate with telomeres via this mechanism could be challenged by factors known to bind directly to the chromosome terminus. This has led to uncertainty as to whether Ku itself binds directly to telomeric ends and whether end association is crucial for Ku's telomeric functions. To address these questions, we constructed DNA end binding-defective Ku heterodimers by altering amino acid residues in Ku70 and Ku80 that were predicted to contact DNA. These mutants continued to associate with their known telomere-related partners, such as Sir4, a factor required for telomeric silencing, and TLC1, the RNA component of telomerase. Despite these interactions, we found that the Ku mutants had markedly reduced association with telomeric chromatin and null-like deficiencies for telomere end protection, length regulation, and silencing functions. In contrast to Ku null strains, the DNA end binding defective Ku mutants resulted in increased, rather than markedly decreased, imprecise end-joining proficiency at an induced double-strand break. This result further supports that it was the specific loss of Ku's telomere end binding that resulted in telomeric defects rather than global loss of Ku's functions. The extensive telomere defects observed in these mutants lead us to propose that Ku is an integral component of the terminal telomeric cap, where it promotes a specific architecture that is central to telomere function and maintenance.

Ku must load directly onto the chromosome end in order to mediate its telomeric functions.

Science.gov (United States)

Lopez, Christopher R; Ribes-Zamora, Albert; Indiviglio, Sandra M; Williams, Christopher L; Haricharan, Svasti; Bertuch, Alison A

2011-08-01

The Ku heterodimer associates with the Saccharomyces cerevisiae telomere, where it impacts several aspects of telomere structure and function. Although Ku avidly binds DNA ends via a preformed channel, its ability to associate with telomeres via this mechanism could be challenged by factors known to bind directly to the chromosome terminus. This has led to uncertainty as to whether Ku itself binds directly to telomeric ends and whether end association is crucial for Ku's telomeric functions. To address these questions, we constructed DNA end binding-defective Ku heterodimers by altering amino acid residues in Ku70 and Ku80 that were predicted to contact DNA. These mutants continued to associate with their known telomere-related partners, such as Sir4, a factor required for telomeric silencing, and TLC1, the RNA component of telomerase. Despite these interactions, we found that the Ku mutants had markedly reduced association with telomeric chromatin and null-like deficiencies for telomere end protection, length regulation, and silencing functions. In contrast to Ku null strains, the DNA end binding defective Ku mutants resulted in increased, rather than markedly decreased, imprecise end-joining proficiency at an induced double-strand break. This result further supports that it was the specific loss of Ku's telomere end binding that resulted in telomeric defects rather than global loss of Ku's functions. The extensive telomere defects observed in these mutants lead us to propose that Ku is an integral component of the terminal telomeric cap, where it promotes a specific architecture that is central to telomere function and maintenance.

Somatic mutation frequency in the stamen hairs of Tradescantia KU 7 and KU 9 clones exposed to low-level gamma rays

International Nuclear Information System (INIS)

Ichikawa, S.; Nagashima, C.; Takahashi, C.S.

1981-01-01

Two triploid clones (KU 7 and KU 9) of Tradescantia heterozygous for flower color were exposed to 1 to 42.3R of gamma rays or the scattering radiation in the gamma field of the Institute of Radiation . Breeding. Occurrence of somatic pink mutations in the stamen hairs was investigated 10 to 16 (or 14) days after irradiation. The mutation frequency was found to increase linearly with increasing gamma-ray exposure in the both clones, and the frequencies of 0.437 and 0.468 pink mutant events per 10 3 hairs per R were determined for KU 7 and KU 9, respectively. When the data collected in the present study were analyzed together with those obtained in earlier experiments in the gamma field, linear relationships of the somatic mutation frequency with gamma-ray (2.1 to 201.6R) and scattering radiation (0.72 to 57.6R) exposures were confirmed. Scattering radiation was found to have a genetical efficiency more than two times higher than that of gamma rays. Variation of spontaneous mutation frequency observed in the present study and in earlier studies was inversely correlated to temperature variation

Multilayered elastic analysis formulation for surface moment loading

CSIR Research Space (South Africa)

Maina, JW

2006-01-01

Full Text Available Denki University Hatoyama-Cho, Hiki-Gun, Saitama 350-0394, JAPAN Tel.: +81-492-96-2911 Fax: +81-492-96-6501 fkiyo@g.dendai.ac.jp Kunihito Matsui Professor Dept. of Civil and Environmental Engineering, Tokyo Denki University Hatoyama-Cho, Hiki...-Gun, Saitama 350-0394, JAPAN Tel.: +81-492-96-2911 Fax: +81-492-96-6501 matsui@g.dendai.ac.jp Takemi Inoue Executive Managing Director NIPPO Corporation 19-11 Kyobashi 1-Chome. Chuo-Ku, Tokyo 104-8380, JAPAN Tel.: +81-3-3563-6760 Fax: +81...

The Ku80 carboxy terminus stimulates joining and artemis-mediated processing of DNA ends

DEFF Research Database (Denmark)

Weterings, Eric; Verkaik, Nicole S; Keijzers, Guido

2008-01-01

Repair of DNA double-strand breaks (DSBs) is predominantly mediated by nonhomologous end joining (NHEJ) in mammalian cells. NHEJ requires binding of the Ku70-Ku80 heterodimer (Ku70/80) to the DNA ends and subsequent recruitment of the DNA-dependent protein kinase catalytic subunit (DNA-PK(CS)) an......Repair of DNA double-strand breaks (DSBs) is predominantly mediated by nonhomologous end joining (NHEJ) in mammalian cells. NHEJ requires binding of the Ku70-Ku80 heterodimer (Ku70/80) to the DNA ends and subsequent recruitment of the DNA-dependent protein kinase catalytic subunit (DNA......-PK(CS)) and the XRCC4/ligase IV complex. Activation of the DNA-PK(CS) serine/threonine kinase requires an interaction with Ku70/80 and is essential for NHEJ-mediated DSB repair. In contrast to previous models, we found that the carboxy terminus of Ku80 is not absolutely required for the recruitment and activation...... was phosphorylated to normal levels. This resulted in severely reduced levels of Artemis nuclease activity in vivo and in vitro. We therefore conclude that the Ku80 carboxy terminus is important to support DNA-PK(CS) autophosphorylation at specific sites, which facilitates DNA end processing by the Artemis...

Expression of Ku70 predicts results of radiotherapy in prostate cancer

Energy Technology Data Exchange (ETDEWEB)

Hasegawa, Tomokazu; Someya, Masanori; Hori, Masakazu; Nakata, Kensei; Kitagawa, Mio; Tsuchiya, Takaaki; Sakata, Koh-ichi [Sapporo Medical University School of medicine, Department of Radiology, Chuo-ku, Sapporo, Hokkaido (Japan); Matsumoto, Yoshihisa [Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology, Meguro-ku, Tokyo (Japan); Nojima, Masanori [The University of Tokyo, The Institute of Medical Science Hospital, Minatoku, Tokyo (Japan); Masumori, Naoya [Sapporo Medical University School of medicine, Department of Urology, Chuo-ku, Sapporo, Hokkaido (Japan); Hasegawa, Tadashi [Sapporo Medical University School of medicine, Department of Surgical Pathology, Chuo-ku, Sapporo, Hokkaido (Japan)

2017-01-15

Therapeutic strategy for prostate cancer is decided according to T stage, Gleason score, and prostate-specific antigen (PSA) level. These clinical factors are not accurate enough to predict individual risk of local failure of prostate cancer after radiotherapy. Parameters involved with radiosensitivity are required to improve the predictive capability for local relapse. We analyzed 58 patients with localized adenocarcinoma of the prostate between August 2007 and October 2010 treated with 76 Gy of intensity-modulated radiotherapy (IMRT) as a discovery cohort and 42 patients between March 2001 and May 2007 treated with three-dimensional conformal radiotherapy (3D-CRT) as a validation cohort. Immunohistochemical examination for proteins involved in nonhomologous end-joining was performed using biopsy specimens. Ku70 expression was not correlated with various clinical parameters, such as the Gleason score and D'amico risk classification, indicating that Ku70 expression was an independent prognostic factor. The predictive value for PSA relapse was markedly improved after the combination of Gleason score and Ku70 expression, as compared with Gleason score alone. In patients treated with radiotherapy and androgen deprivation therapy (ADT), no relapses were observed in patients with Gleason score ≤7 or low Ku70 expression. In contrast, patients with Gleason score ≥8 and high Ku70 expression had high PSA relapse rates. In the validation cohort, similar results were obtained. Treatment with 76 Gy and ADT can be effective for patients with Gleason score ≤7 or low Ku70 expression, but is not enough for patients with Gleason score ≥8 and high Ku70 expression and, thus, require other treatment approaches. (orig.) [German] Die Behandlung beim Prostatakarzinom ist abhaengig von T-Stadium, Gleason-Score und prostataspezifischem Antigen (PSA). Diese klinischen Faktoren sind jedoch zu ungenau, um das individuelle Lokalrezidivrisiko beim Prostatakarzinom nach

Mycobacterium tuberculosis Ku can bind to nuclear DNA damage and sensitize mammalian cells to bleomycin sulfate.

Science.gov (United States)

Castore, Reneau; Hughes, Cameron; Debeaux, Austin; Sun, Jingxin; Zeng, Cailing; Wang, Shih-Ya; Tatchell, Kelly; Shi, Runhua; Lee, Kyung-Jong; Chen, David J; Harrison, Lynn

2011-11-01

Radiotherapy and chemotherapy are effective cancer treatments due to their ability to generate DNA damage. The major lethal lesion is the DNA double-strand break (DSB). Human cells predominantly repair DSBs by non-homologous end joining (NHEJ), which requires Ku70, Ku80, DNA-PKcs, DNA ligase IV and accessory proteins. Repair is initiated by the binding of the Ku heterodimer at the ends of the DSB and this recruits DNA-PKcs, which initiates damage signaling and functions in repair. NHEJ also exists in certain types of bacteria that have dormant phases in their life cycle. The Mycobacterium tuberculosis Ku (Mt-Ku) resembles the DNA-binding domain of human Ku but does not have the N- and C-terminal domains of Ku70/80 that have been implicated in binding mammalian NHEJ repair proteins. The aim of this work was to determine whether Mt-Ku could be used as a tool to bind DSBs in mammalian cells and sensitize cells to DNA damage. We generated a fusion protein (KuEnls) of Mt-Ku, EGFP and a nuclear localization signal that is able to perform bacterial NHEJ and hence bind DSBs. Using transient transfection, we demonstrated that KuEnls is able to bind laser damage in the nucleus of Ku80-deficient cells within 10 sec and remains bound for up to 2 h. The Mt-Ku fusion protein was over-expressed in U2OS cells and this increased the sensitivity of the cells to bleomycin sulfate. Hydrogen peroxide and UV radiation do not predominantly produce DSBs and there was little or no change in sensitivity to these agents. Since in vitro studies were unable to detect binding of Mt-Ku to DNA-PKcs or human Ku70/80, this work suggests that KuEnls sensitizes cells by binding DSBs, preventing human NHEJ. This study indicates that blocking or decreasing the binding of human Ku to DSBs could be a method for enhancing existing cancer treatments.

Deletion of individual Ku subunits in mice causes an NHEJ-independent phenotype potentially by altering apurinic/apyrimidinic site repair.

Directory of Open Access Journals (Sweden)

Yong Jun Choi

Full Text Available Ku70 and Ku80 form a heterodimer called Ku that forms a holoenzyme with DNA dependent-protein kinase catalytic subunit (DNA-PKCS to repair DNA double strand breaks (DSBs through the nonhomologous end joining (NHEJ pathway. As expected mutating these genes in mice caused a similar DSB repair-defective phenotype. However, ku70(-/- cells and ku80(-/- cells also appeared to have a defect in base excision repair (BER. BER corrects base lesions, apurinic/apyrimidinic (AP sites and single stand breaks (SSBs utilizing a variety of proteins including glycosylases, AP endonuclease 1 (APE1 and DNA Polymerase β (Pol β. In addition, deleting Ku70 was not equivalent to deleting Ku80 in cells and mice. Therefore, we hypothesized that free Ku70 (not bound to Ku80 and/or free Ku80 (not bound to Ku70 possessed activity that influenced BER. To further test this hypothesis we performed two general sets of experiments. The first set showed that deleting either Ku70 or Ku80 caused an NHEJ-independent defect. We found ku80(-/- mice had a shorter life span than dna-pkcs(-/- mice demonstrating a phenotype that was greater than deleting the holoenzyme. We also found Ku70-deletion induced a p53 response that reduced the level of small mutations in the brain suggesting defective BER. We further confirmed that Ku80-deletion impaired BER via a mechanism that was not epistatic to Pol β. The second set of experiments showed that free Ku70 and free Ku80 could influence BER. We observed that deletion of either Ku70 or Ku80, but not both, increased sensitivity of cells to CRT0044876 (CRT, an agent that interferes with APE1. In addition, free Ku70 and free Ku80 bound to AP sites and in the case of Ku70 inhibited APE1 activity. These observations support a novel role for free Ku70 and free Ku80 in altering BER.

Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in prostate cancer.

Science.gov (United States)

Evans-Axelsson, Susan; Vilhelmsson Timmermand, Oskar; Welinder, Charlotte; Borrebaeck, Carl Ak; Strand, Sven-Erik; Tran, Thuy A; Jansson, Bo; Bjartell, Anders

2014-01-01

The aim of this investigation was to assess the Ku70/Ku80 complex as a potential target for antibody imaging of prostate cancer. We evaluated the in vivo and ex vivo tumor targeting and biodistribution of the (111)In-labeled human internalizing antibody, INCA-X ((111)In-DTPA-INCA-X antibody), in NMRI-nude mice bearing human PC-3, PC-3M-Lu2 or DU145 xenografts. DTPA-conjugated, non-labeled antibody was pre-administered at different time-points followed by a single intravenous injection of (111)In-DTPA-INCA-X. At 48, 72 and 96 h post-injection, tissues were harvested, and the antibody distribution was determined by measuring radioactivity. Preclinical SPECT/CT imaging of mice with and without the predose was performed at 48 hours post-injection of labeled DTPA-INCA-X. Biodistribution of the labeled antibody showed enriched activity in tumor, spleen and liver. Animals pre-administered with DTPA-INCA-X showed increased tumor uptake and blood content of (111)In-DTPA-INCA-X with reduced splenic and liver uptake. The in vitro and in vivo data presented show that the (111)In-labeled INCA-X antibody is internalized into prostate cancer cells and by pre-administering non-labeled DTPA-INCA-X, we were able to significantly reduce the off target binding and increase the (111)In-DTPA-INCA-X mAb uptake in PC-3, PC-3M-Lu2 and DU145 xenografts. The results are encouraging and identifying the Ku70/Ku80 antigen as a target is worth further investigation for functional imaging of prostate cancer.

Preclinical evaluation of 111In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in prostate cancer

Science.gov (United States)

Evans-Axelsson, Susan; Vilhelmsson Timmermand, Oskar; Welinder, Charlotte; Borrebaeck, Carl AK; Strand, Sven-Erik; Tran, Thuy A; Jansson, Bo; Bjartell, Anders

2014-01-01

The aim of this investigation was to assess the Ku70/Ku80 complex as a potential target for antibody imaging of prostate cancer. We evaluated the in vivo and ex vivo tumor targeting and biodistribution of the 111In-labeled human internalizing antibody, INCA-X (111In-DTPA-INCA-X antibody), in NMRI-nude mice bearing human PC-3, PC-3M-Lu2 or DU145 xenografts. DTPA-conjugated, non-labeled antibody was pre-administered at different time-points followed by a single intravenous injection of 111In-DTPA-INCA-X. At 48, 72 and 96 h post-injection, tissues were harvested, and the antibody distribution was determined by measuring radioactivity. Preclinical SPECT/CT imaging of mice with and without the predose was performed at 48 hours post-injection of labeled DTPA-INCA-X. Biodistribution of the labeled antibody showed enriched activity in tumor, spleen and liver. Animals pre-administered with DTPA-INCA-X showed increased tumor uptake and blood content of 111In-DTPA-INCA-X with reduced splenic and liver uptake. The in vitro and in vivo data presented show that the 111In-labeled INCA-X antibody is internalized into prostate cancer cells and by pre-administering non-labeled DTPA-INCA-X, we were able to significantly reduce the off target binding and increase the 111In-DTPA-INCA-X mAb uptake in PC-3, PC-3M-Lu2 and DU145 xenografts. The results are encouraging and identifying the Ku70/Ku80 antigen as a target is worth further investigation for functional imaging of prostate cancer. PMID:24982817

Ku recruits XLF to DNA double-strand breaks.

Science.gov (United States)

Yano, Ken-ichi; Morotomi-Yano, Keiko; Wang, Shih-Ya; Uematsu, Naoya; Lee, Kyung-Jong; Asaithamby, Aroumougame; Weterings, Eric; Chen, David J

2008-01-01

XRCC4-like factor (XLF)--also known as Cernunnos--has recently been shown to be involved in non-homologous end-joining (NHEJ), which is the main pathway for the repair of DNA double-strand breaks (DSBs) in mammalian cells. XLF is likely to enhance NHEJ by stimulating XRCC4-ligase IV-mediated joining of DSBs. Here, we report mechanistic details of XLF recruitment to DSBs. Live cell imaging combined with laser micro-irradiation showed that XLF is an early responder to DSBs and that Ku is essential for XLF recruitment to DSBs. Biochemical analysis showed that Ku-XLF interaction occurs on DNA and that Ku stimulates XLF binding to DNA. Unexpectedly, XRCC4 is dispensable for XLF recruitment to DSBs, although photobleaching analysis showed that XRCC4 stabilizes the binding of XLF to DSBs. Our observations showed the direct involvement of XLF in the dynamic assembly of the NHEJ machinery and provide mechanistic insights into DSB recognition.

RECQ HELICASE RECQL4 PARTICIPATES IN NON-HOMOLOGOUS END JOINING AND INTERACTS WITH THE KU COMPLEX

DEFF Research Database (Denmark)

Shamanna, Raghavendra A; Singh, Dharmendra Kumar; Lu, Huiming

2014-01-01

-irradiation and resulted in accumulation of 53BP1 foci after irradiation, indicating defects in the processing of DSB. We find that RECQL4 interacts with the Ku70/Ku80 heterodimer, part of the DNA-dependent protein kinase (DNA-PK) complex, via its N-terminal domain. Further, RECQL4 stimulates higher order DNA binding...... of Ku70/Ku80 to a blunt end DNA substrate. Taken together, these results implicate that RECQL4 participates in the NHEJ pathway of DSB repair via a functional interaction with the Ku70/Ku80 complex. This is the first study to provide both in vitro and in vivo evidence for a role of a RecQ helicase...

Construction of cell model of silenced Ku80 and the radiobiology change of HeLa cell

International Nuclear Information System (INIS)

Zhuang Liang; Yu Shiying; Huang Xiaoyuan; Xiong Huihua; Xiong Hua; Li Xiaolan; Leng Yan

2007-01-01

Objective: To construct the cell model of Ku80 with expression inhibited by siRNA and to explore the role of Ku80 in radiobiology. Methods: Ku80-siRNA expression plasmids were constructed and HeLa cells were transfected with these plasmids by lipofectamine. Western blotting was used to measure the expression of Ku80. After irradiation with 6 MV X ray, cells were collected and analyzed by flow cytometry for apoptosis and cell cycle at 24, 48 and 72 h; The radiobiology parameters of four cell lines were acquired by clone formation array. Results: Three stable transfected cell clones were obtained, and the inhibition rates of Ku80 protein expression of two positive clones were 89.3% and 96.4%; The apoptosis rates of HeLa cells Ku80 inhibited were higher than control cells at 48 and 72 after X ray irradiation (P 0.05). HeLa cells of silenced Ku80 had lower SF 2 and D 0 than control cells, and their SER (sensitization enhancement ratio) based on D 10 were 1.315 and 1.365, respectively. Conclusions: The HeLa cell models with Ku80 expression suppressed were successfully established; the inhibition of Ku80 by siRNA could enhance the radiosensitivity of HeLa cells. (authors)

The Ku80 carboxy terminus stimulates joining and artemis-mediated processing of DNA ends.

Science.gov (United States)

Weterings, Eric; Verkaik, Nicole S; Keijzers, Guido; Florea, Bogdan I; Wang, Shih-Ya; Ortega, Laura G; Uematsu, Naoya; Chen, David J; van Gent, Dik C

2009-03-01

Repair of DNA double-strand breaks (DSBs) is predominantly mediated by nonhomologous end joining (NHEJ) in mammalian cells. NHEJ requires binding of the Ku70-Ku80 heterodimer (Ku70/80) to the DNA ends and subsequent recruitment of the DNA-dependent protein kinase catalytic subunit (DNA-PK(CS)) and the XRCC4/ligase IV complex. Activation of the DNA-PK(CS) serine/threonine kinase requires an interaction with Ku70/80 and is essential for NHEJ-mediated DSB repair. In contrast to previous models, we found that the carboxy terminus of Ku80 is not absolutely required for the recruitment and activation of DNA-PK(CS) at DSBs, although cells that harbored a carboxy-terminal deletion in the Ku80 gene were sensitive to ionizing radiation and showed reduced end-joining capacity. More detailed analysis of this repair defect showed that DNA-PK(CS) autophosphorylation at Thr2647 was diminished, while Ser2056 was phosphorylated to normal levels. This resulted in severely reduced levels of Artemis nuclease activity in vivo and in vitro. We therefore conclude that the Ku80 carboxy terminus is important to support DNA-PK(CS) autophosphorylation at specific sites, which facilitates DNA end processing by the Artemis endonuclease and the subsequent joining reaction.

The Ku80 Carboxy Terminus Stimulates Joining and Artemis-Mediated Processing of DNA Ends▿

Science.gov (United States)

Weterings, Eric; Verkaik, Nicole S.; Keijzers, Guido; Florea, Bogdan I.; Wang, Shih-Ya; Ortega, Laura G.; Uematsu, Naoya; Chen, David J.; van Gent, Dik C.

2009-01-01

Repair of DNA double-strand breaks (DSBs) is predominantly mediated by nonhomologous end joining (NHEJ) in mammalian cells. NHEJ requires binding of the Ku70-Ku80 heterodimer (Ku70/80) to the DNA ends and subsequent recruitment of the DNA-dependent protein kinase catalytic subunit (DNA-PKCS) and the XRCC4/ligase IV complex. Activation of the DNA-PKCS serine/threonine kinase requires an interaction with Ku70/80 and is essential for NHEJ-mediated DSB repair. In contrast to previous models, we found that the carboxy terminus of Ku80 is not absolutely required for the recruitment and activation of DNA-PKCS at DSBs, although cells that harbored a carboxy-terminal deletion in the Ku80 gene were sensitive to ionizing radiation and showed reduced end-joining capacity. More detailed analysis of this repair defect showed that DNA-PKCS autophosphorylation at Thr2647 was diminished, while Ser2056 was phosphorylated to normal levels. This resulted in severely reduced levels of Artemis nuclease activity in vivo and in vitro. We therefore conclude that the Ku80 carboxy terminus is important to support DNA-PKCS autophosphorylation at specific sites, which facilitates DNA end processing by the Artemis endonuclease and the subsequent joining reaction. PMID:19103741

The Ku Heterodimer and the Metabolism of Single-Ended DNA Double-Strand Breaks

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Alessia Balestrini

2013-06-01

Full Text Available Single-ended double-strand breaks (DSBs are a common form of spontaneous DNA break, generated when the replisome encounters a discontinuity in the DNA template. Given their prevalence, understanding the mechanisms governing the fate(s of single-ended DSBs is important. We describe the influence of the Ku heterodimer and Mre11 nuclease activity on processing of single-ended DSBs. Separation-of-function alleles of yku70 were derived that phenocopy Ku deficiency with respect to single-ended DSBs but remain proficient for NHEJ. The Ku mutants fail to regulate Exo1 activity, and bypass the requirement for Mre11 nuclease activity in the repair of camptothecin-induced single-ended DSBs. Ku mutants exhibited reduced affinity for DNA ends, manifest as both reduced end engagement and enhanced probability of diffusing inward on linear DNA. This study reveals an interplay between Ku and Mre11 in the metabolism of single-ended DSBs that is distinct from repair pathway choice at double-ended DSBs.

Ku proteins function as corepressors to regulate farnesoid X receptor-mediated gene expression

International Nuclear Information System (INIS)

Ohno, Masae; Kunimoto, Masaaki; Nishizuka, Makoto; Osada, Shigehiro; Imagawa, Masayoshi

2009-01-01

The farnesoid X receptor (FXR; NR1H4) is a member of the nuclear receptor superfamily and regulates the expression of genes involved in enterohepatic circulation and the metabolism of bile acids. Based on functional analyses, nuclear receptors are divided into regions A-F. To explore the cofactors interacting with FXR, we performed a pull-down assay using GST-fused to the N-terminal A/B region and the C region, which are required for the ligand-independent transactivation and DNA-binding, respectively, of FXR, and nuclear extracts from HeLa cells. We identified DNA-dependent protein kinase catalytic subunit (DNA-PKcs), Ku80, and Ku70 as FXR associated factors. These proteins are known to have an important role in DNA repair, recombination, and transcription. DNA-PKcs mainly interacted with the A/B region of FXR, whereas the Ku proteins interacted with the C region and with the D region (hinge region). Chromatin immunoprecipitation assays revealed that the Ku proteins associated with FXR on the bile salt export pump (BSEP) promoter. Furthermore, we demonstrated that ectopic expression of the Ku proteins decreased the promoter activity and expression of BSEP gene mediated by FXR. These results suggest that the Ku proteins function as corepressors for FXR.

The Ku heterodimer and the metabolism of single-ended DNA double-strand breaks.

Science.gov (United States)

Balestrini, Alessia; Ristic, Dejan; Dionne, Isabelle; Liu, Xiao Z; Wyman, Claire; Wellinger, Raymund J; Petrini, John H J

2013-06-27

Single-ended double-strand breaks (DSBs) are a common form of spontaneous DNA break, generated when the replisome encounters a discontinuity in the DNA template. Given their prevalence, understanding the mechanisms governing the fate(s) of single-ended DSBs is important. We describe the influence of the Ku heterodimer and Mre11 nuclease activity on processing of single-ended DSBs. Separation-of-function alleles of yku70 were derived that phenocopy Ku deficiency with respect to single-ended DSBs but remain proficient for NHEJ. The Ku mutants fail to regulate Exo1 activity, and bypass the requirement for Mre11 nuclease activity in the repair of camptothecin-induced single-ended DSBs. Ku mutants exhibited reduced affinity for DNA ends, manifest as both reduced end engagement and enhanced probability of diffusing inward on linear DNA. This study reveals an interplay between Ku and Mre11 in the metabolism of single-ended DSBs that is distinct from repair pathway choice at double-ended DSBs. Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi's sarcoma-associated herpesvirus latent replication

International Nuclear Information System (INIS)

Cha, Seho; Lim, Chunghun; Lee, Jae Young; Song, Yoon-Jae; Park, Junsoo; Choe, Joonho; Seo, Taegun

2010-01-01

During latent infection, latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) plays important roles in episomal persistence and replication. Several host factors are associated with KSHV latent replication. Here, we show that the catalytic subunit of DNA protein kinase (DNA-PKcs), Ku70, and Ku86 bind the N-terminal region of LANA. LANA was phosphorylated by DNA-PK and overexpression of Ku70, but not Ku86, impaired transient replication. The efficiency of transient replication was significantly increased in the HCT116 (Ku86 +/-) cell line, compared to the HCT116 (Ku86 +/+) cell line, suggesting that the DNA-PK/Ku complex negatively regulates KSHV latent replication.

Airborn Ku-band polarimetric radar remote sensing of terrestrial snow cover

Science.gov (United States)

Simon H. Yueh; Steve J. Dinardo; Ahmed Akgiray; Richard West; Donald W. Cline; Kelly Elder

2009-01-01

Characteristics of the Ku-band polarimetric scatterometer (POLSCAT) data acquired from five sets of aircraft flights in the winter months of 2006-2008 for the second Cold Land Processes Experiment (CLPX-II) in Colorado are described in this paper. The data showed the response of the Ku-band radar echoes to snowpack changes for various types of background vegetation in...

Drosophila UTX coordinates with p53 to regulate ku80 expression in response to DNA damage.

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Chengwan Zhang

Full Text Available UTX is known as a general factor that activates gene transcription during development. Here, we demonstrate an additional essential role of UTX in the DNA damage response, in which it upregulates the expression of ku80 in Drosophila, both in cultured cells and in third instar larvae. We further showed that UTX mediates the expression of ku80 by the demethylation of H3K27me3 at the ku80 promoter upon exposure to ionizing radiation (IR in a p53-dependent manner. UTX interacts physically with p53, and both UTX and p53 are recruited to the ku80 promoter following IR exposure in an interdependent manner. In contrast, the loss of utx has little impact on the expression of ku70, mre11, hid and reaper, suggesting the specific regulation of ku80 expression by UTX. Thus, our findings further elucidate the molecular function of UTX.

C-terminal low-complexity sequence repeats of Mycobacterium smegmatis Ku modulate DNA binding.

Science.gov (United States)

Kushwaha, Ambuj K; Grove, Anne

2013-01-24

Ku protein is an integral component of the NHEJ (non-homologous end-joining) pathway of DSB (double-strand break) repair. Both eukaryotic and prokaryotic Ku homologues have been characterized and shown to bind DNA ends. A unique feature of Mycobacterium smegmatis Ku is its basic C-terminal tail that contains several lysine-rich low-complexity PAKKA repeats that are absent from homologues encoded by obligate parasitic mycobacteria. Such PAKKA repeats are also characteristic of mycobacterial Hlp (histone-like protein) for which they have been shown to confer the ability to appose DNA ends. Unexpectedly, removal of the lysine-rich extension enhances DNA-binding affinity, but an interaction between DNA and the PAKKA repeats is indicated by the observation that only full-length Ku forms multiple complexes with a short stem-loop-containing DNA previously designed to accommodate only one Ku dimer. The C-terminal extension promotes DNA end-joining by T4 DNA ligase, suggesting that the PAKKA repeats also contribute to efficient end-joining. We suggest that low-complexity lysine-rich sequences have evolved repeatedly to modulate the function of unrelated DNA-binding proteins.

Shuttle orbiter Ku-band radar/communications system design evaluation. Deliverable test equipment evaluation

Science.gov (United States)

Maronde, R. G.

1980-07-01

The Ku-band test equipment, known as the Deliverable System Test equipment (DSTE), is reviewed and evaluated. The DSTE is semiautomated and computer programs were generated for 14 communication mode tests and 17 radar mode tests. The 31 test modules provide a good cross section of tests with which to exercise the Ku-band system; however, it is very limited when being used to verify Ku-band system performance. More detailed test descriptions are needed, and a major area of concern is the DSTE sell-off procedure which is inadequate.

Improving DNA double-strand repair inhibitor KU55933 therapeutic index in cancer radiotherapy using nanoparticle drug delivery

Science.gov (United States)

Tian, Xi; Lara, Haydee; Wagner, Kyle T.; Saripalli, Srinivas; Hyder, Syed Nabeel; Foote, Michael; Sethi, Manish; Wang, Edina; Caster, Joseph M.; Zhang, Longzhen; Wang, Andrew Z.

2015-11-01

Radiotherapy is a key component of cancer treatment. Because of its importance, there has been high interest in developing agents and strategies to further improve the therapeutic index of radiotherapy. DNA double-strand repair inhibitors (DSBRIs) are among the most promising agents to improve radiotherapy. However, their clinical translation has been limited by their potential toxicity to normal tissue. Recent advances in nanomedicine offer an opportunity to overcome this limitation. In this study, we aim to demonstrate the proof of principle by developing and evaluating nanoparticle (NP) formulations of KU55933, a DSBRI. We engineered a NP formulation of KU55933 using nanoprecipitation method with different lipid polymer nanoparticle formulation. NP KU55933 using PLGA formulation has the best loading efficacy as well as prolonged drug release profile. We demonstrated that NP KU55933 is a potent radiosensitizer in vitro using clonogenic assay and is more effective as a radiosensitizer than free KU55933 in vivo using mouse xenograft models of non-small cell lung cancer (NSCLC). Western blots and immunofluorescence showed NP KU55933 exhibited more prolonged inhibition of DNA repair pathway. In addition, NP KU55933 leads to lower skin toxicity than KU55933. Our study supports further investigations using NP to deliver DSBRIs to improve cancer radiotherapy treatment.

Development of intense terahertz coherent synchrotron radiation at KU-FEL

Energy Technology Data Exchange (ETDEWEB)

Sei, Norihiro, E-mail: sei.n@aist.go.jp [Research Institute for Measurement and Analytical Instrumentation, National Institute of Advanced Industrial Science and Technology, 1-1-1 Umezono, Umezono, Tsukuba, Ibaraki 305-8568 (Japan); Zen, Heishun; Ohgaki, Hideaki [Institute for Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011 (Japan)

2016-10-01

We produced intense coherent synchrotron radiation (CSR) in the terahertz (THz) region using an S-band linac at the Kyoto University Free Electron Laser (KU-FEL), which is a mid-infrared free-electron laser facility. The CSR beam was emitted from short-pulse electron bunches compressed by a 180° arc, and was transferred to air at a large solid angle of 0.10 rad. The measured CSR energy was 55 μJ per 7 μs macropulse, and KU-FEL was one of the most powerful CSR sources in normal conducting linear accelerator facilities. The CSR spectra were measured using an uncooled pyroelectric detector and a Michelson-type interferometer designed specifically for the KU-FEL electron beam, and had a maximum at a frequency of 0.11 THz. We found that adjusting the energy slit enhanced the CSR energy and shortened the electron beam bunch length in the CSR spectra measurements. Our results demonstrated that the efficient use of the energy slit can help improve the characteristics of CSR. - Highlights: • We have developed intense coherent synchrotron radiation (CSR) at KU-FEL. • The elevation angle of the CSR was correctly measured by a new technique. • The CSR power extracted to the air was 55 μJ per 7 μs macropulse. • It was demonstrated that an energy slit was effective to improve the CSR properties.

Development of intense terahertz coherent synchrotron radiation at KU-FEL

International Nuclear Information System (INIS)

Sei, Norihiro; Zen, Heishun; Ohgaki, Hideaki

2016-01-01

We produced intense coherent synchrotron radiation (CSR) in the terahertz (THz) region using an S-band linac at the Kyoto University Free Electron Laser (KU-FEL), which is a mid-infrared free-electron laser facility. The CSR beam was emitted from short-pulse electron bunches compressed by a 180° arc, and was transferred to air at a large solid angle of 0.10 rad. The measured CSR energy was 55 μJ per 7 μs macropulse, and KU-FEL was one of the most powerful CSR sources in normal conducting linear accelerator facilities. The CSR spectra were measured using an uncooled pyroelectric detector and a Michelson-type interferometer designed specifically for the KU-FEL electron beam, and had a maximum at a frequency of 0.11 THz. We found that adjusting the energy slit enhanced the CSR energy and shortened the electron beam bunch length in the CSR spectra measurements. Our results demonstrated that the efficient use of the energy slit can help improve the characteristics of CSR. - Highlights: • We have developed intense coherent synchrotron radiation (CSR) at KU-FEL. • The elevation angle of the CSR was correctly measured by a new technique. • The CSR power extracted to the air was 55 μJ per 7 μs macropulse. • It was demonstrated that an energy slit was effective to improve the CSR properties.

Double strand break rejoining by the Ku-dependent mechanism of non-homologous end-joining

International Nuclear Information System (INIS)

Jeggo, P.; Singleton, B.; Beamish, H.; Priestley, A.

1999-01-01

The DNA-dependent protein kinase functions in the repair of DNA double strand breaks (DSBs) and in V(D)J recombination. To gain insight into the function of DNA-PK in this process we have carried out a mutation analysis of Ku80 and DNA-PKcs. Mutations at multiple sites within the N-terminal two thirds of Ku80 result in loss of Ku70/80 interaction, loss of DNA end-binding activity and inability to complement Ku80 defective cell fines. In contrast, mutations in the carboxy terminal region of the protein do not impair DNA end-binding activity but decrease the ability of Ku to activate DNA-PK. To gain insight into important functional domains within DNA-PKcs, we have analysed defective mutants, including the mouse scid cell line, and the rodent mutants, ire-20 and V-3. Mutational changes in the carboxy terminal region have been identified in all cases. Our results strongly suggest that the C-terminus of DNA-PKcs is required for kinase activity. (author)

Vecāku un bērnu mantiskās attiecības

OpenAIRE

Krauja, Uldis

2009-01-01

Bakalaura darba "Vecāku un bērna mantiskās attiecības" mērķis ir izpētīt vecāku un bērna mantisko attiecību regulējumu Latvijas Republikas normatīvajos tiesību aktos, atklājot tajos iespējamās problēmas un nepilnības. Bakalaura darba pirmajā daļā apskatīts vecāku un bērna mantisko attiecību jēdziens un izpratne, pievērsta uzmanība uz vecāku un bērna mantisko attiecību pirmsākumiem caur vēstures prizmu, lai apzinātos ģimenes tiesību nozīmīgumu cilvēces attīstībā un tagadējā dzīvē. Bakalaura da...

Synthetic lethality between murine DNA repair factors XLF and DNA-PKcs is rescued by inactivation of Ku70

DEFF Research Database (Denmark)

Xing, Mengtan; Bjørås, Magnar; Daniel, Jeremy A

2017-01-01

DNA double-strand breaks (DSBs) are recognized and repaired by the Classical Non-Homologous End-Joining (C-NHEJ) and Homologous Recombination pathways. C-NHEJ includes the core Ku70 and Ku80 (or Ku86) heterodimer that binds DSBs and thus promotes recruitment of accessory downstream NHEJ factors XLF......, PAXX, DNA-PKcs, Artemis and other core subunits, XRCC4 and DNA Ligase 4 (Lig4). In the absence of core C-NHEJ factors, DNA repair can be performed by Alternative End-Joining, which likely depends on DNA Ligase 1 and DNA Ligase 3. Genetic inactivation of C-NHEJ factors, such as Ku70, Ku80, XLF, PAXX...... with severe apoptosis in the central nervous system. Here, we demonstrate that inactivation of the Ku70 gene rescues the synthetic lethality between XLF and DNA-PKcs, resulting in triple knockout mice that are indistinguishable from Ku70-deficient littermates by size or levels of genomic instability. Moreover...

Downregulated Ku70 and ATM associated to poor prognosis in colorectal cancer among Chinese patients

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Lu YF

2014-10-01

Full Text Available Yuanfang Lu,1,2 Jingyan Gao,1,3 Yuanming Lu,1 1Department of Toxicology, School of Public Health, Guilin Medical University, Guangxi, People's Republic of China; 2Department of Clinical Research Center, Affiliated 2nd Hospital of Nanjing Medical University, Nanjing, People's Republic of China; 3Department of Human Anatomy and Histo-Embryology, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China Background: Double-strand DNA breaks (DSBs are a key factor in carcinogenesis. The necessary repair of DSBs is pivotal in maintaining normal cell division. To address the relationship between altered expression of DSB repair of proteins Ku70 and ataxia-telangiectasia mutated (ATM in colorectal cancer (CRC, we examined the expression levels and patterns of Ku70 and ATM in CRC samples. Methods: Expression and coexpression of Ku70 and ATM were investigated by using real-time quantitative polymerase chain reaction assays and confirmed further with fluorescent immunohistochemistry in CRC and pericancerous samples from 112 Chinese patients. Results: Downexpression patterns for both Ku70 and ATM were found in the CRC samples and were significantly associated with advanced tumor node metastasis stage and decreased 5-year overall survival rate. Conclusion: Downregulated Ku70 and ATM were associated with poor disease-free survival. Loss of Ku70 and ATM expression might act as a biomarker to predict poor prognosis in patients with CRC. Keywords: DNA double-strand breaks, ataxia-telangiectasia mutated, Ku70, colorectal cancer

Ku-mediated coupling of DNA cleavage and repair during programmed genome rearrangements in the ciliate Paramecium tetraurelia.

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Antoine Marmignon

2014-08-01

Full Text Available During somatic differentiation, physiological DNA double-strand breaks (DSB can drive programmed genome rearrangements (PGR, during which DSB repair pathways are mobilized to safeguard genome integrity. Because of their unique nuclear dimorphism, ciliates are powerful unicellular eukaryotic models to study the mechanisms involved in PGR. At each sexual cycle, the germline nucleus is transmitted to the progeny, but the somatic nucleus, essential for gene expression, is destroyed and a new somatic nucleus differentiates from a copy of the germline nucleus. In Paramecium tetraurelia, the development of the somatic nucleus involves massive PGR, including the precise elimination of at least 45,000 germline sequences (Internal Eliminated Sequences, IES. IES excision proceeds through a cut-and-close mechanism: a domesticated transposase, PiggyMac, is essential for DNA cleavage, and DSB repair at excision sites involves the Ligase IV, a specific component of the non-homologous end-joining (NHEJ pathway. At the genome-wide level, a huge number of programmed DSBs must be repaired during this process to allow the assembly of functional somatic chromosomes. To understand how DNA cleavage and DSB repair are coordinated during PGR, we have focused on Ku, the earliest actor of NHEJ-mediated repair. Two Ku70 and three Ku80 paralogs are encoded in the genome of P. tetraurelia: Ku70a and Ku80c are produced during sexual processes and localize specifically in the developing new somatic nucleus. Using RNA interference, we show that the development-specific Ku70/Ku80c heterodimer is essential for the recovery of a functional somatic nucleus. Strikingly, at the molecular level, PiggyMac-dependent DNA cleavage is abolished at IES boundaries in cells depleted for Ku80c, resulting in IES retention in the somatic genome. PiggyMac and Ku70a/Ku80c co-purify as a complex when overproduced in a heterologous system. We conclude that Ku has been integrated in the Paramecium

Volba idolů ve školním věku (Pop-Pantheon)

OpenAIRE

Ryba, Zdeněk

2016-01-01

Title: Idolization In The School Age (Pop-Pantheon) Author: Zdeněk Ryba Department: Department of Psychology Supervizor: doc. PhDr. Miloš Kučera, CSc. The main theme of this thesis is basic-school-age children choice of idols. The work is a part of Miloš Kučera research at Faculty of Education UK and it follows up the Mikšíček (1999) and Kravcová (2008) conclusions in their diploma thesis and short Kučera's contribution. The goal of this thesis is to make a database of idols and to create mod...

Uloga kućnih ljubimaca u socioemocionalnom  razvoju djece školskog uzrasta

OpenAIRE

Smojver-Ažić, Sanja; Topalović, Zorica

2010-01-01

Rezultati istraživanja potvrđuju kako je odrastanje uz kućnoga ljubimca povezano sa socioemocionalnim razvojem djece. Cilj ovoga istraživanja bio je ispitati odnos u kojemu stoje privrženost majci, privrženost kućnomu ljubimcu i empatija, socijalna kompetencija i usamljenost. U istraživanju je sudjelovalo 480 učenika četvrtoga, šestoga i osmoga razreda osnovnih škola u Istri (226 djevojčica i 254 dječaka) te njihovi roditelji. U ispitanom uzorku 63 % djece ima jednoga ili više kućnih ljubi...

The Ku70/80 ring in Non-Homologous End-Joining

DEFF Research Database (Denmark)

Kragelund, Birthe Brandt; Weterings, Eric; Hartmann-Petersen, Rasmus

2016-01-01

/80 heterodimer is a key-player in the NHEJ pathway and binds to DNA termini with high affinity, where it helps to protect DNA ends from degradation and to recruit other NHEJ factors required for repair. The mechanism of Ku70/80 detachment from the DNA helix after completion of DNA repair is incompletely......Non-homologous end-joining (NHEJ) is an essential DNA double strand break repair pathway during all cell cycle stages. Deficiency in NHEJ factors can lead to accumulation of unrepaired DNA breaks or faulty DNA repair, which may ultimately result in cell death, senescence or carcinogenesis. The Ku70...... understood. Some data suggest that certain DNA repair factors are ubiquitylated and targeted for proteasomal degradation after repair. Recent studies suggest that Ku80 is conjugated to lysine48-linked ubiquitin chains by the Skp1-Cullin-F-box (SCF) complex and/or the RING finger protein 8 (RNF8) ubiquitin-protein...

Xrcc1-dependent and Ku-dependent DNA double-strand break repair kinetics in Arabidopsis plants.

Science.gov (United States)

Charbonnel, Cyril; Gallego, Maria E; White, Charles I

2010-10-01

Double-strand breakage (DSB) of DNA involves loss of information on the two strands of the DNA fibre and thus cannot be repaired by simple copying of the complementary strand which is possible with single-strand DNA damage. Homologous recombination (HR) can precisely repair DSB using another copy of the genome as template and non-homologous recombination (NHR) permits repair of DSB with little or no dependence on DNA sequence homology. In addition to the well-characterised Ku-dependent non-homologous end-joining (NHEJ) pathway, much recent attention has been focused on Ku-independent NHR. The complex interrelationships and regulation of NHR pathways remain poorly understood, even more so in the case of plants, and we present here an analysis of Ku-dependent and Ku-independent repair of DSB in Arabidopsis thaliana. We have characterised an Arabidopsis xrcc1 mutant and developed quantitative analysis of the kinetics of appearance and loss of γ-H2AX foci as a tool to measure DSB repair in dividing root tip cells of γ-irradiated plants in vivo. This approach has permitted determination of DSB repair kinetics in planta following a short pulse of γ-irradiation, establishing the existence of a Ku-independent, Xrcc1-dependent DSB repair pathway. Furthermore, our data show a role for Ku80 during the first minutes post-irradiation and that Xrcc1 also plays such a role, but only in the absence of Ku. The importance of Xrcc1 is, however, clearly visible at later times in the presence of Ku, showing that alternative end-joining plays an important role in DSB repair even in the presence of active NHEJ. © 2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd.

A novel small molecule inhibitor of the DNA repair protein Ku70/80.

Science.gov (United States)

Weterings, Eric; Gallegos, Alfred C; Dominick, Lauren N; Cooke, Laurence S; Bartels, Trace N; Vagner, Josef; Matsunaga, Terry O; Mahadevan, Daruka

2016-07-01

Non-Homologous End-Joining (NHEJ) is the predominant pathway for the repair of DNA double strand breaks (DSBs) in human cells. The NHEJ pathway is frequently upregulated in several solid cancers as a compensatory mechanism for a separate DSB repair defect or for innate genomic instability, making this pathway a powerful target for synthetic lethality approaches. In addition, NHEJ reduces the efficacy of cancer treatment modalities which rely on the introduction of DSBs, like radiation therapy or genotoxic chemotherapy. Consequently, inhibition of the NHEJ pathway can modulate a radiation- or chemo-refractory disease presentation. The Ku70/80 heterodimer protein plays a pivotal role in the NHEJ process. It possesses a ring-shaped structure with high affinity for DSBs and serves as the first responder and central scaffold around which the rest of the repair complex is assembled. Because of this central position, the Ku70/80 dimer is a logical target for the disruption of the entire NHEJ pathway. Surprisingly, specific inhibitors of the Ku70/80 heterodimer are currently not available. We here describe an in silico, pocket-based drug discovery methodology utilizing the crystal structure of the Ku70/80 heterodimer. We identified a novel putative small molecule binding pocket and selected several potential inhibitors by computational screening. Subsequent biological screening resulted in the first identification of a compound with confirmed Ku-inhibitory activity in the low micro-molar range, capable of disrupting the binding of Ku70/80 to DNA substrates and impairing Ku-dependent activation of another NHEJ factor, the DNA-PKCS kinase. Importantly, this compound synergistically sensitized human cell lines to radiation treatment, indicating a clear potential to diminish DSB repair. The chemical scaffold we here describe can be utilized as a lead-generating platform for the design and development of a novel class of anti-cancer agents. Copyright © 2016 Elsevier B.V. All

Molecular characterization of resistant chronic lymphocytic leukemia (CLL): function of a new phosphorylated form of Ku70

International Nuclear Information System (INIS)

Saad, Lina

2013-01-01

We have identified a new form of phospho-S27-S33-Ku70 constitutively over expressed in a subset of chronic lymphocytic leukemia (CLL) B cells resistant to apoptosis induced by DNA double strand breaks (DSB). Ku70 is one of the essential proteins involved in the maintenance of genomic stability through its role in DNA double strand break repair (non-homologous end-joining, NHEJ) and in telomeric protection. Laboratory previously established that resistant CLL cells disclose an up-regulated NHEJ DNA repair and an impaired structure of telomeres. The goal of this thesis was to characterize the biological function(s) of this new form of Ku70. For this purpose we have constructed specific EBV-based vectors (siRNA / cDNA) enabling a simultaneous inhibition of endogenous Ku70 and an expression of different forms (mutated, wild, phospho-mimetic at ser27-33) of Ku70 resistant to siRNA. Thus, we showed: i) a strict co-localisation of phospho-Ku70 with γ-H2AX foci; ii) that DSB induces the phosphorylation of Ku70 within minutes after genotoxic stress in heterodimer complex Ku70/Ku80. This phosphorylation necessitates both the physical interaction and the activity of pS2056-DNA-PKcs and/or ATM, linking phospho-Ku70 to NHEJ-mediated DNA DSB repair; iii) cells expressing mutated A27-A33-Ku70 exhibit a delayed G2/M cell cycle arrest, slower kinetic of DNA repair, lower level of genotoxic stress-induced chromosomal aberrations, and a higher cellular impedance correlated with translocation of transcriptional factor β-catenin from cytoplasmic membrane to the nucleus. Together, these data unveil an involvement of phospho-Ku70 in fast and inaccurate DNA repair; new paradigm for NHEJ regulation and to the control of resistance and maintenance of malignant cells.In parallel, we have initiated experimental approaches to explore other potential roles of phospho-Ku70. Especially, we were interested to determine whether it could play a role in an initiation of cell senescence induced by

DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi's sarcoma-associated herpesvirus latent replication

Energy Technology Data Exchange (ETDEWEB)

Cha, Seho [Department of Life Science, Dongguk Univ-Seoul, Seoul 100-715 (Korea, Republic of); Lim, Chunghun [Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701 (Korea, Republic of); Lee, Jae Young [Department of Life Science, Dongguk Univ-Seoul, Seoul 100-715 (Korea, Republic of); Song, Yoon-Jae [Department of Life Science, Kyungwon University, Seongnam-Si, Kyeonggi-Do 461-701 (Korea, Republic of); Park, Junsoo [Division of Biological Science and Technology, Yonsei University, Wonju 220-100 (Korea, Republic of); Choe, Joonho [Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701 (Korea, Republic of); Seo, Taegun, E-mail: tseo@dongguk.edu [Department of Life Science, Dongguk Univ-Seoul, Seoul 100-715 (Korea, Republic of)

2010-04-16

During latent infection, latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) plays important roles in episomal persistence and replication. Several host factors are associated with KSHV latent replication. Here, we show that the catalytic subunit of DNA protein kinase (DNA-PKcs), Ku70, and Ku86 bind the N-terminal region of LANA. LANA was phosphorylated by DNA-PK and overexpression of Ku70, but not Ku86, impaired transient replication. The efficiency of transient replication was significantly increased in the HCT116 (Ku86 +/-) cell line, compared to the HCT116 (Ku86 +/+) cell line, suggesting that the DNA-PK/Ku complex negatively regulates KSHV latent replication.

Functional Interplay of the Mre11 Nuclease and Ku in the Response to Replication-Associated DNA Damage ▿

Science.gov (United States)

Foster, Steven S.; Balestrini, Alessia; Petrini, John H. J.

2011-01-01

The Mre11 complex is a central component of the DNA damage response, with roles in damage sensing, molecular bridging, and end resection. We have previously shown that in Saccharomyces cerevisiae, Ku70 (yKu70) deficiency reduces the ionizing radiation sensitivity of mre11Δ mutants. In this study, we show that yKu70 deficiency suppressed the camptothecin (CPT) and methyl methanesulfonate (MMS) sensitivity of nuclease-deficient mre11-3 and sae2Δ mutants in an Exo1-dependent manner. CPT-induced G2/M arrest, γ-H2AX persistence, and chromosome breaks were elevated in mre11-3 mutants. These outcomes were reduced by yKu70 deficiency. Given that the genotoxic effects of CPT are manifest during DNA replication, these data suggest that Ku limits Exo1-dependent double-strand break (DSB) resection during DNA replication, inhibiting the initial processing steps required for homology-directed repair. We propose that Mre11 nuclease- and Sae2-dependent DNA end processing, which initiates DSB resection prevents Ku from engaging DSBs, thus promoting Exo1-dependent resection. In agreement with this idea, we show that Ku affinity for binding to short single-stranded overhangs is much lower than for blunt DNA ends. Collectively, the data define a nonhomologous end joining (NHEJ)-independent, S-phase-specific function of the Ku heterodimer. PMID:21876003

Interplay between Ku and Replication Protein A in the Restriction of Exo1-mediated DNA Break End Resection*

Science.gov (United States)

Krasner, Danielle S.; Daley, James M.; Sung, Patrick; Niu, Hengyao

2015-01-01

DNA double-strand breaks can be eliminated via non-homologous end joining or homologous recombination. Non-homologous end joining is initiated by the association of Ku with DNA ends. In contrast, homologous recombination entails nucleolytic resection of the 5′-strands, forming 3′-ssDNA tails that become coated with replication protein A (RPA). Ku restricts end access by the resection nuclease Exo1. It is unclear how partial resection might affect Ku engagement and Exo1 restriction. Here, we addressed these questions in a reconstituted system with yeast proteins. With blunt-ended DNA, Ku protected against Exo1 in a manner that required its DNA end-binding activity. Despite binding poorly to ssDNA, Ku could nonetheless engage a 5′-recessed DNA end with a 40-nucleotide (nt) ssDNA overhang, where it localized to the ssDNA-dsDNA junction and efficiently blocked resection by Exo1. Interestingly, RPA could exclude Ku from a partially resected structure with a 22-nt ssDNA tail and thus restored processing by Exo1. However, at a 40-nt tail, Ku remained stably associated at the ssDNA-dsDNA junction, and RPA simultaneously engaged the ssDNA region. We discuss a model in which the dynamic equilibrium between Ku and RPA binding to a partially resected DNA end influences the timing and efficiency of the resection process. PMID:26067273

Serum anti-Ku86 is a potential biomarker for early detection of hepatitis C virus-related hepatocellular carcinoma

Energy Technology Data Exchange (ETDEWEB)

Nomura, Fumio, E-mail: fnomura@faculty.chiba-u.jp [Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University and Divisions of Laboratory Medicine, Clinical Genetics and Proteomics, Chiba University Hospital, Chiba (Japan); Sogawa, Kazuyuki; Noda, Kenta; Seimiya, Masanori; Matsushita, Kazuyuki; Miura, Toshihide [Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University and Divisions of Laboratory Medicine, Clinical Genetics and Proteomics, Chiba University Hospital, Chiba (Japan); Tomonaga, Takeshi [Laboratory of Proteome Research, National Institute of Biomedical Innovation, Ibaraki (Japan); Yoshitomi, Hideyuki [Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba (Japan); Imazeki, Fumio [Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, Chiba (Japan); Takizawa, Hirotaka [Kashiwado Clinic in Port-Square of the Kashiwado Memorial Foundation, Chiba (Japan); Mogushi, Kaoru [Information Center for Medical Sciences, Tokyo Dental and Medical University, Tokyo (Japan); Miyazaki, Masaru [Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba (Japan); Yokosuka, Osamu [Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, Chiba (Japan)

2012-05-18

Highlights: Black-Right-Pointing-Pointer Overexpression of Ku86 in human liver cancer was shown by immunohistochemistry. Black-Right-Pointing-Pointer Serum anti-Ku86 was significantly elevated in early hepatocellular carcinoma. Black-Right-Pointing-Pointer Anti-Ku86 may be more sensitive than the conventional markers for early detection. Black-Right-Pointing-Pointer Serum anti-Ku86 significantly decreased after surgical resection of liver tumors. Black-Right-Pointing-Pointer Elevation of serum anti-Ku86 in other non-liver solid tumors was minimal. -- Abstract: Hepatocellular carcinoma (HCC), the predominant form of primary liver cancer, is one of the most common cancers worldwide and the third most common cause of cancer-related death. Imaging studies including ultrasound and computed tomography are recommended for early detection of HCC, but they are operator dependent, costly and involve radiation. Therefore, there is a need for simple and sensitive serum markers for the early detection of hepatocellular carcinoma (HCC). In our recent proteomic studies, a number of proteins overexpressed in HCC tissues were identified. We thought if the serum autoantibodies to these overexpressed proteins were detectable in HCC patients. Of these proteins, we focused on Ku86, a nuclear protein involved in multiple biological processes and aimed to assess the diagnostic value of serum anti-Ku86 in the early detection of HCC. Serum samples were obtained prior to treatment from 58 consecutive patients with early or relatively early hepatitis C virus (HCV)-related HCC and 137 patients with HCV-related liver cirrhosis without evidence of HCC. Enzyme immunoassays were used to measure serum levels of autoantibodies. Serum levels of anti-Ku86 antibodies were significantly elevated in HCC patients compared to those in liver cirrhosis patients (0.41 {+-} 0.28 vs. 0.18 {+-} 0.08 Abs at 450 nm, P < 0001). Setting the cut-off level to give 90% specificity, anti-Ku86 was positive in 60.7% of

Radiosensitizing activity and pharmacokinetics of multiple dose administered KU-2285 in peripheral nerve tissue in mice

International Nuclear Information System (INIS)

Iwai, Hiroyuki; Matsuno, Etsuko; Sasai, Keisuke; Abe, Mitsuyuki; Shibamoto, Yuta

1994-01-01

In a clinical trial in which a 2-nitroimidazole radiosensitizer was administered repeatedly, the dose-limiting toxicity was found to be peripheral neuropathy. In the present study, the in vivo radiosensitizing activity of KU-2285 in combination with radiation dose fractionation, and the pharmacokinetics of cumulative dosing of KU-2285 in the peripheral nerves were examined. The ability of three nitroimidazoles, misonidazole (MISO), etanidazole (SR-2508) and KU-2285, to sensitize SCCVII tumors to radiation treatment has been compared for drug doses in the range 0-200 mg/kg. Single radiation doses or two different fractionation schedules (6 Gy/fractions x three fractions/48 h or 5 Gy/fractions x five fractions/48 h) were used; the tumor cell survival was determined using an in vivo/in vitro colony assay. The pharmacokinetics in the sciatic nerves were undertaken, when KU-2285 or etanidazole were injected at a dose of 200 mg/kg intravenously one, two, three, or four times at 2-h intervals. At less than 100 mg/kg, KU-2285 sensitized SCCVII tumors more than MISO and SR-2508 by fractionated irradiation. Evaluation of pharmacokinetics in the peripheral nerves showed that the apparent biological half-life of SR-2508 increased with the increases in the number of administrations, whereas that of KU-2285 became shorter. Since most clinical radiotherapy is given in small multiple fractions, KU-2285 appears to be a hypoxic cell radiosensitizer that could be useful in such regimens, and that poses no risk of chronic peripheral neurotoxicity. 12 refs., 5 figs., 1 tab

Increased expression of IRE1α and stress-related signal transduction proteins in ischemia-reperfusion injured retina

Directory of Open Access Journals (Sweden)

Natsuyo Hata

2008-08-01

Full Text Available Natsuyo Hata1, Toshiyuki Oshitari1,2, Akiko Yokoyama1,3, Yoshinori Mitamura1, Shuichi Yamamoto11Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, Chuo-ku, Chiba, Japan; 2Department of Ophthalmology, Kimitsu Central Hospital, Kisarazu City, Chiba, Japan; 3Department of Ophthalmology, Inoue Memorial Hospital, Chuo-ku, Chiba, JapanAbstract: The purpose of this study was to determine whether the expression of ER stress-related factors IRE1α, apoptosis signal-regulating kinase 1 (ASK1, SAPK/ERK kinase 1 (SEK1 and c-Jun N-terminal kinase (JNK is associated with the damaged retinal neurons induced by ischemia-reperfusion injury. After 60 minutes of ischemia, the rat retinas were reperfused, and retinas were isolated and fixed after 6, 9, 12, 18, and 24 hours, and 2, 5, and 9 days of reperfusion. Cryosections were immunostained with Fluoro-Jade B, a degenerating neuron marker to label degenerating neurons. Semi-quantitative analysis of the expression of IRE1α, ASK1, SEK1, and JNK were performed in both control and ischemic retinas. In ischemic retinas, the intensities of IRE1α immunoreactivity in the ganglion cell layer (GCL were significantly higher than in the control retinas. In ischemic retinas, the numbers of SEK1-, ASK1-, and JNK-positive cells were significantly increased in the GCL compared to those in the control retinas. In addition, the cells that were positive for SEK1-, ASK1-, and JNK were also positive for Fluoro-Jade B-positive cells. These results indicate that the increased expression of ER stress-related factors was, in part, associated with the retinal neuronal abnormalities after ischemia-reperfusion injury in rat retinas.Keywords: endoplasmic reticulum, IRE1α, apoptosis signal-regulating kinase 1, SAPK/ERK kinase 1, c-Jun N-terminal kinase, Fluoro-Jade B, ischemia-reperfusion injury

Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA

International Nuclear Information System (INIS)

Smider, V.; Rathmell, W.K.; Chu, G.; Lieber, M.R.

1994-01-01

Three genetic complementation groups of rodent cells are defective for both repair of x-ray-induced double-strand breaks and V(D)J recombination. Cells from one group lack a DNA end-binding activity that is biochemically and antigenically similar to the Ku autoantigen. Transfection of complementary DNA (cDNA) that encoded the 86-kilodalton subunit of Ku rescued these mutant cells for DNA end-binding activity, x-ray resistance, and V(D)J recombination activity. These results establish a role for Ku in DNA repair and recombination. Furthermore, as a component of a DNA-dependent protein kinase, Ku may initiate a signaling pathway induced by DNA damage

Deletion of individual Ku subunits in mice causes an NHEJ-independent phenotype potentially by altering apurinic/apyrimidinic site repair

NARCIS (Netherlands)

Y.J. Choi (Yong Jun); H. Li (Han); M.Y. Son (Mi Young); X.-H. Wang (Xiao-Hong); J.L. Fornsaglio (Jamie L.); R.W. Sobol (Robert W.); M. Lee (Moonsook); J. Vijg (Jan); S. Imholz (Sandra); M.E.T. Dollé (Martijn); H. van Steeg (Harry); E. Reiling (Erwin); P. Hasty (Paul)

2014-01-01

textabstractKu70 and Ku80 form a heterodimer called Ku that forms a holoenzyme with DNA dependent-protein kinase catalytic subunit (DNA-PKCS) to repair DNA double strand breaks (DSBs) through the nonhomologous end joining (NHEJ) pathway. As expected mutating these genes in mice caused a similar DSB

18F-fluorodeoxyglucose positron emission tomography might be useful for diagnosis of hepatic amyloidosis

Directory of Open Access Journals (Sweden)

Tawada A

2014-06-01

Full Text Available Akinobu Tawada,1 Tatsuo Kanda,1 Takashi Oide,2 Toshio Tsuyuguchi,1 Fumio Imazeki,1,3 Yukio Nakatani,2 Osamu Yokosuka11Department of Gastroenterology, 2Department of Diagnostic Pathology, Chiba University Hospital, Chuo-ku, Chiba, Japan; 3Safety and Health Organization, Chiba University, Inage-ku, Chiba, JapanAbstract: We report on a woman with hepatic involvement of primary systemic (immunoglobulin light chain, AL amyloidosis. Her diagnosis was confirmed by liver biopsy. Clinical symptoms of hepatic amyloidosis are generally mild at its first stage, with most frequent findings being hepatomegaly and alkaline phosphatase elevation. Recent advances in the understanding of the pathophysiology of systemic amyloidosis have made several treatments available. However, its prognosis is occasionally poor. Because liver biopsy is not always safe, other modalities for the diagnosis are needed. Of interest was that fluorodeoxyglucose (FDG uptake into the liver was observed, compared with that into the spleen, in this patient, indicating that FDG positron emission tomography and computed tomography might be useful for the diagnosis of hepatic amyloidosis with mild liver dysfunction.Keywords: amyloidosis, diagnosis, hepatic involvement, FDG PET

GPM, DPR Level 2A Ku Precipitation V03

Data.gov (United States)

National Aeronautics and Space Administration — The 2AKu algorithm provides precipitation estimates from the Ku radar of the Dual-Frequency Precipitation Radar on the core GPM spacecraft. The product contains one...

Pohon pásového dopravníku pro transport uhlí

OpenAIRE

Kliš, Kamil

2013-01-01

Diplomová práce se zabývá návrhem pohonu pásového dopravníku pro transport uhlí. Práce obsahuje nezbytné výpočty dle norem potřebné k určení vhodných komponentů pásového dopravníku. Cílem diplomové práce bylo navrhnout pohon pásového dopravníku, všech jeho potřebných komponentů včetně návrhem rámu pohonu a vytvoření výkresové dokumentace. This diploma thesis deals with designing a drivetrain for a coal belt conveyor. Thesis contains necessary calculations according to norms needed for dete...

A Ku-band magnetically insulated transmission line oscillator with overmoded slow-wave-structure

Science.gov (United States)

Jiang, Tao; He, Jun-Tao; Zhang, Jian-De; Li, Zhi-Qiang; Ling, Jun-Pu

2016-12-01

In order to enhance the power capacity, an improved Ku-band magnetically insulated transmission line oscillator (MILO) with overmoded slow-wave-structure (SWS) is proposed and investigated numerically and experimentally. The analysis of the dispersion relationship and the resonant curve of the cold test indicate that the device can operate at the near π mode of the TM01 mode, which is useful for mode selection and control. In the particle simulation, the improved Ku-band MILO generates a microwave with a power of 1.5 GW and a frequency of 12.3 GHz under an input voltage of 480 kV and input current of 42 kA. Finally, experimental investigation of the improved Ku-band MILO is carried out. A high-power microwave (HPM) with an average power of 800 MW, a frequency of 12.35 GHz, and pulse width of 35 ns is generated under a diode voltage of 500 kV and beam current of 43 kA. The consistency between the experimental and simulated far-field radiation pattern confirms that the operating mode of the improved Ku-band MILO is well controlled in π mode of the TM01 mode. Project supported partly by the National Natural Science Foundation of China (Grant No. 61171021).

The Ku70/80 ring in Non-Homologous End-Joining: easy to slip on, hard to remove.

Science.gov (United States)

Kragelund, Birthe B; Weterings, Eric; Hartmann-Petersen, Rasmus; Keijzers, Guido

2016-01-01

Non-homologous end-joining (NHEJ) is an essential DNA double strand break repair pathway during all cell cycle stages. Deficiency in NHEJ factors can lead to accumulation of unrepaired DNA breaks or faulty DNA repair, which may ultimately result in cell death, senescence or carcinogenesis. The Ku70/80 heterodimer is a key-player in the NHEJ pathway and binds to DNA termini with high affinity, where it helps to protect DNA ends from degradation and to recruit other NHEJ factors required for repair. The mechanism of Ku70/80 detachment from the DNA helix after completion of DNA repair is incompletely understood. Some data suggest that certain DNA repair factors are ubiquitylated and targeted for proteasomal degradation after repair. Recent studies suggest that Ku80 is conjugated to lysine48-linked ubiquitin chains by the Skp1-Cullin-F-box (SCF) complex and/or the RING finger protein 8 (RNF8) ubiquitin-protein ligases, followed by rapid proteasomal degradation. In this review we address the structure and function of the Ku70/80 heterodimer and how ubiquitylation may affect the release of Ku70/80 from chromatin and its subsequent degradation via the ubiquitin-proteasome system.

An integrated Ka/Ku-band payload for personal, mobile and private business communications

Science.gov (United States)

Hayes, Edward J.; Keelty, J. Malcolm

1991-01-01

The Canadian Department of Communications has been studying options for a government-sponsored demonstration payload to be launched before the end of the century. A summary of the proposed system concepts and network architectures for providing an advanced private business network service at Ku-band and personal and mobile communications at Ka-band is presented. The system aspects addressed include coverage patterns, traffic capacity, and grade of service, multiple access options as well as special problems, such as Doppler in mobile applications. Earth terminal types and the advanced payload concept proposed in a feasibility study for the demonstration mission are described. This concept is a combined Ka-band/Ku-band payload which incorporates a number of advanced satellite technologies including a group demodulator to convert single-channel-per-carrier frequency division multiple access uplink signals to a time division multiplex downlink, on-board signal regeneration, and baseband switching to support packet switched data operation. The on-board processing capability of the payload provides a hubless VSAT architecture which permits single-hop full mesh interconnectivity. The Ka-band and Ku-band portions of the payload are fully integrated through an on-board switch, thereby providing the capability for fully integrated services, such as using the Ku-band VSAT terminals as gateway stations for the Ka-band personal and mobile communications services.

Coexposure to benzo[a]pyrene plus UVA induced DNA double strand breaks: visualization of Ku assembly in the nucleus having DNA lesions

International Nuclear Information System (INIS)

Toyooka, Tatsushi; Ibuki, Yuko; Koike, Manabu; Ohashi, Norio; Takahashi, Sentaro; Goto, Rensuke

2004-01-01

Benzo[a]pyrene (BaP) is a ubiquitous environmental pollutant with potential carcinogenicity. It has been shown that BaP, upon UVA irradiation, synergistically induced oxidative DNA damage, but other DNA damage was not confirmed. In this study, we examined whether coexposure to BaP plus UVA induces double strand breaks (DSBs) using xrs-5 cells, deficient in the repair of DSBs (Ku80 mutant), and whether Ku translocates involving the formation of DSBs. BaP plus UVA had a significant cytotoxic effect on CHO-K1 cells and an even more drastic effect on Ku80-deficient, xrs-5 cells, suggesting that the DSBs were generated by coexposure to BaP plus UVA. The DSBs were repaired in CHO-K1 cells within 30 min, but not in xrs-5 cells, indicating the involvement of a non-homologous end joining, which needs Ku proteins. Furthermore, we succeeded in visualizing that Ku80 rapidly assembled to the exposed region, in which DSBs might be generated, and clarified that the presence of both Ku70 and Ku80 was important for their accumulation

Radiation effects on radiation-hardened KU and KS-4V optical fibres

International Nuclear Information System (INIS)

Ivanov, A.A.; Tugarinov, S.N.; Kaschuck, Y.A.; Krasilnikov, A.V.; Bender, S.E.

1999-01-01

The aim of this work was to test the un-pretreated and the hardened (H 2 -loaded and pre-irradiated) KS-4V and KU optical fibres in reactor environment by in-situ measurements of both the radiation-induced loss and the luminescence in the visible spectral region. Both the radio-luminescent and the transmission spectra were in-situ detected during irradiation by charge-coupled-device (CCD) linear detector in the visible spectral region of 400 to 700 nm. The radiation induced loss spectra at the fast neutron fluence of 2*10 6 n/cm 2 shows the hardened, H 2 -loading and pre-irradiating effects in the both KU and KS-4V fibres. KU un-pretreated fibre shows a big radiation absorption band of non-bridging oxygen centered at the wavelength of 630 nm. It appears that the KS-4V hardened fibre has a specific point in the loss spectrum in the vicinity of 460 nm. Other measurements were performed, particularly after reactor shutdown and at 3 different neutron fluences with constant neutron flux after restarting

Hypoxia downregulates Ku70/80 expression in cervical carcinoma tumors

International Nuclear Information System (INIS)

Lara, Pedro Carlos; Lloret, Marta; Clavo, Bernardino; Apolinario, Rosa Maria; Bordon, Elisa; Rey, Agustin; Falcon, Orlando; Alonso, Ana Ruiz; Belka, Claus

2008-01-01

Hypoxia may inhibits the NHEJ DNA repair through downregulating Ku70/80 expression and combined with an increased angiogenesis and altered p53 expression would be responsible for tumor progression in cervical carcinoma

The Ku Protein Complex Interacts with YY1, Is Up-Regulated in Human Heart Failure, and Represses α Myosin Heavy-Chain Gene Expression

Science.gov (United States)

Sucharov, Carmen C.; Helmke, Steve M.; Langer, Stephen J.; Perryman, M. Benjamin; Bristow, Michael; Leinwand, Leslie

2004-01-01

Human heart failure is accompanied by repression of genes such as α myosin heavy chain (αMyHC) and SERCA2A and the induction of fetal genes such as βMyHC and atrial natriuretic factor. It seems likely that changes in MyHC isoforms contribute to the poor contractility seen in heart failure, because small changes in isoform composition can have a major effect on the contractility of cardiac myocytes and the heart. Our laboratory has recently shown that YY1 protein levels are increased in human heart failure and that YY1 represses the activity of the human αMyHC promoter. We have now identified a region of the αMyHC promoter that binds a factor whose expression is increased sixfold in failing human hearts. Through peptide mass spectrometry, we identified this binding activity to be a heterodimer of Ku70 and Ku80. Expression of Ku represses the human αMyHC promoter in neonatal rat ventricular myocytes. Moreover, overexpression of Ku70/80 decreases αMyHC mRNA expression and increases skeletal α-actin. Interestingly, YY1 interacts with Ku70 and Ku80 in HeLa cells. Together, YY1, Ku70, and Ku80 repress the αMyHC promoter to an extent that is greater than that with YY1 or Ku70/80 alone. Our results suggest that Ku is an important factor in the repression of the human αMyHC promoter during heart failure. PMID:15367688

Alterations in cellular energy metabolism associated with the antiproliferative effects of the ATM inhibitor KU-55933 and with metformin.

Directory of Open Access Journals (Sweden)

Mahvash Zakikhani

Full Text Available KU-55933 is a specific inhibitor of the kinase activity of the protein encoded by Ataxia telangiectasia mutated (ATM, an important tumor suppressor gene with key roles in DNA repair. Unexpectedly for an inhibitor of a tumor suppressor gene, KU-55933 reduces proliferation. In view of prior preliminary evidence suggesting defective mitochondrial function in cells of patients with Ataxia Telangiectasia (AT, we examined energy metabolism of cells treated with KU-55933. The compound increased AMPK activation, glucose uptake and lactate production while reducing mitochondrial membrane potential and coupled respiration. The stimulation of glycolysis by KU-55933 did not fully compensate for the reduction in mitochondrial functions, leading to decreased cellular ATP levels and energy stress. These actions are similar to those previously described for the biguanide metformin, a partial inhibitor of respiratory complex I. Both compounds decreased mitochondrial coupled respiration and reduced cellular concentrations of fumarate, malate, citrate, and alpha-ketogluterate. Succinate levels were increased by KU-55933 levels and decreased by metformin, indicating that the effects of ATM inhibition and metformin are not identical. These observations suggest a role for ATM in mitochondrial function and show that both KU-55933 and metformin perturb the TCA cycle as well as oxidative phosphorylation.

Klimatizace líhně kuřat

OpenAIRE

Petr, Lukáš

2009-01-01

Diplomová práce se zabývá návrhem systému klimatizačního zařízení pro prostory s líhněmi kuřat. Práce v úvodu krátce přibližuje problematiku klimatizace, představuje dispozice objektu a nastiňuje požadavky na mikroklima pro správnou inkubaci vajec a líhnutí kuřat. Praktická část diplomové práce se dělí na dva základní úseky, a to výpočtový a návrhový. Výpočtová část se zabývá stanovením množství přiváděného čerstvého klimatizačního vzduchu, výpočtem tepelných ztrát, tepelné zátěže a psychrome...

Role of Ku80-dependent end-joining in delayed genomic instability in mammalian cells surviving ionizing radiation

International Nuclear Information System (INIS)

Suzuki, Keiji; Kodama, Seiji; Watanabe, Masami

2010-01-01

Ionizing radiation induces delayed destabilization of the genome in the progenies of surviving cells. This phenomenon, which is called radiation-induced genomic instability, is manifested by delayed induction of radiation effects, such as cell death, chromosome aberration, and mutation in the progeny of cells surviving radiation exposure. Previously, there was a report showing that delayed cell death was absent in Ku80-deficient Chinese hamster ovary (CHO) cells, however, the mechanism of their defect has not been determined. We found that delayed induction of DNA double strand breaks and chromosomal breaks were intact in Ku80-deficient cells surviving X-irradiation, whereas there was no sign for the production of chromosome bridges between divided daughter cells. Moreover, delayed induction of dicentric chromosomes was significantly compromised in those cells compared to the wild-type CHO cells. Reintroduction of the human Ku86 gene complimented the defective DNA repair and recovered delayed induction of dicentric chromosomes and delayed cell death, indicating that defective Ku80-dependent dicentric induction was the cause of the absence of delayed cell death. Since DNA-PKcs-defective cells showed delayed phenotypes, Ku80-dependent illegitimate rejoining is involved in delayed impairment of the integrity of the genome in radiation-survived cells.

Role of Ku80-dependent end-joining in delayed genomic instability in mammalian cells surviving ionizing radiation

Energy Technology Data Exchange (ETDEWEB)

Suzuki, Keiji, E-mail: kzsuzuki@nagasaki-u.ac.jp [Course of Life Sciences and Radiation Research, Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523 (Japan); Kodama, Seiji [Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1-2 Gakuen-machi, Sakai 599-8570 (Japan); Watanabe, Masami [Kyoto University Research Reactor Institute, Kumatori-cho Sennan-gun, Osaka 590-0494 (Japan)

2010-01-05

Ionizing radiation induces delayed destabilization of the genome in the progenies of surviving cells. This phenomenon, which is called radiation-induced genomic instability, is manifested by delayed induction of radiation effects, such as cell death, chromosome aberration, and mutation in the progeny of cells surviving radiation exposure. Previously, there was a report showing that delayed cell death was absent in Ku80-deficient Chinese hamster ovary (CHO) cells, however, the mechanism of their defect has not been determined. We found that delayed induction of DNA double strand breaks and chromosomal breaks were intact in Ku80-deficient cells surviving X-irradiation, whereas there was no sign for the production of chromosome bridges between divided daughter cells. Moreover, delayed induction of dicentric chromosomes was significantly compromised in those cells compared to the wild-type CHO cells. Reintroduction of the human Ku86 gene complimented the defective DNA repair and recovered delayed induction of dicentric chromosomes and delayed cell death, indicating that defective Ku80-dependent dicentric induction was the cause of the absence of delayed cell death. Since DNA-PKcs-defective cells showed delayed phenotypes, Ku80-dependent illegitimate rejoining is involved in delayed impairment of the integrity of the genome in radiation-survived cells.

Návrh zásobníku na sypký vápenný hydrát

OpenAIRE

Perna, Marek

2009-01-01

Tato diplomová práce řeší návrh hlubokého zásobníku na sypký vápenný hydrát pro technologická zařízení čistíren odpadních vod. Je zde proveden rozbor volby typu a konstrukce zásobníku, návrh rozměrů a pevnostní výpočet zásobníku. Je zde popsána možnost vzniku klenby při vyprazdňování a její potlačení pomocí aktivních a pasivních prvků napomáhající toku. Popis aktivních a pasivních stavů napjatosti materiálu v zásobníku. This diploma thesis is solving systems design of silo for a powdery li...

Loss of MLH1 sensitizes colon cancer cells to DNA-PKcs inhibitor KU60648.

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Hinrichsen, Inga; Ackermann, Anne; Düding, Tonja; Graband, Annika; Filmann, Natalie; Plotz, Guido; Zeuzem, Stefan; Brieger, Angela

2017-07-01

Germline mutations of MLH1 are responsible for tumor generation in nearly 50% of patients with Lynch Syndrome, and around 15% of sporadic colorectal cancers show MLH1-deficiency due to promotor hypermethylation. Although these tumors are of lower aggressiveness the benefit for these patients from standard chemotherapy is still under discussion. Recently, it was shown that the sensitivity to the DNA-PKcs inhibitor KU60648 is linked to loss of the MMR protein MSH3. However, loss of MSH3 is rather secondary, as a consequence of MMR-deficiency, and frequently detectable in MLH1-deficient tumors. Therefore, we examined the expression of MLH1, MSH2, MSH6, and MSH3 in different MMR-deficient and proficient cell lines and determined their sensitivity to KU60648 by analyzing cell viability and survival. MLH1-dependent ability of double strand break (DSB) repair was monitored after irradiation via γH2AX detection. A panel of 12 colon cancer cell lines, two pairs of cells, where MLH1 knock down was compared to controls with the same genetic background, and one MLH1-deficient cell line where MLH1 was overexpressed, were included. In summary, we found that MLH1 and/or MSH3-deficient cells exhibited a significantly higher sensitivity to KU60648 than MMR-proficient cells and that overexpression of MLH1 in MLH1-deficient cells resulted in a decrease of cell sensitivity. KU60648 efficiency seems to be associated with reduced DSB repair capacity. Since the molecular testing of colon tumors for MLH1 expression is a clinical standard we believe that MLH1 is a much better marker and a greater number of patients would benefit from KU60648 treatment. © 2017 Wiley Periodicals, Inc.

Experimental research on Ku-band magnetically insulated transmission line oscillator

Energy Technology Data Exchange (ETDEWEB)

Jiang, Tao; Zhang, Jiande; He, Juntao; Li, Zhiqiang; Ling, Junpu [College of Optoelectric Science and Engineering, National University of Defense Technology, Hunan 410073 (China)

2015-10-15

An improved Ku-band magnetically insulated transmission line oscillator is proposed and investigated experimentally. In the particle-in-cell simulation, the Ku-band MILO generates the microwave with a power of 1.62 GW and a frequency of 13 GHz at the input voltage of 474 kV. The device is fabricated based on the simulation results, and an experiment system is designed. In the preliminary experiments, output microwave with frequency of 13.02 GHz, power of 150 MW, and pulse width of 17 ns is generated, under the diode voltage of 450 kV. Analysis on the experiment results shows that plasma produced due to the large current hitting to the outside of the collection tank is the essential cause for the low amplitude of the microwave power and short pulse width.

Mre11 and Blm-Dependent Formation of ALT-Like Telomeres in Ku-Deficient Ustilago maydis.

Directory of Open Access Journals (Sweden)

Eun Young Yu

2015-10-01

Full Text Available A subset of human cancer cells uses a specialized, aberrant recombination pathway known as ALT to maintain telomeres, which in these cells are characterized by complex aberrations including length heterogeneity, high levels of unpaired C-strand, and accumulation of extra-chromosomal telomere repeats (ECTR. These phenotypes have not been recapitulated in any standard budding or fission yeast mutant. We found that eliminating Ku70 or Ku80 in the yeast-like fungus Ustilago maydis results initially in all the characteristic telomere aberrations of ALT cancer cells, including C-circles, a highly specific marker of ALT. Subsequently the ku mutants experience permanent G2 cell cycle arrest, accompanied by loss of telomere repeats from chromosome ends and even more drastic accumulation of very short ECTRs (vsECTRs. The deletion of atr1 or chk1 rescued the lethality of the ku mutant, and "trapped" the telomere aberrations in the early ALT-like stage. Telomere abnormalities are telomerase-independent, but dramatically suppressed by deletion of mre11 or blm, suggesting major roles for these factors in the induction of the ALT pathway. In contrast, removal of other DNA damage response and repair factors such as Rad51 has disparate effects on the ALT phenotypes, suggesting that these factors process ALT intermediates or products. Notably, the antagonism of Ku and Mre11 in the induction of ALT is reminiscent of their roles in DSB resection, in which Blm is also known to play a key role. We suggest that an aberrant resection reaction may constitute an early trigger for ALT telomeres, and that the outcomes of ALT are distinct from DSB because of the unique telomere nucleoprotein structure.

Mycobacterial UvrD1 is a Ku-dependent DNA helicase that plays a role in multiple DNA repair events, including double-strand break repair.

Science.gov (United States)

Sinha, Krishna Murari; Stephanou, Nicolas C; Gao, Feng; Glickman, Michael S; Shuman, Stewart

2007-05-18

Mycobacterium tuberculosis and other bacterial pathogens have a Ku-dependent nonhomologous end joining pathway of DNA double-strand break repair. Here we identify mycobacterial UvrD1 as a novel interaction partner for Ku in a genome-wide yeast two-hybrid screen. UvrD1 per se is a vigorous DNA-dependent ATPase but a feeble DNA helicase. Ku stimulates UvrD1 to catalyze ATP-dependent unwinding of 3'-tailed DNAs. UvrD1, Ku, and DNA form a stable ternary complex in the absence of ATP. The Ku binding determinants are located in the distinctive C-terminal segment of UvrD1. A second mycobacterial paralog, UvrD2, is a vigorous Ku-independent DNA helicase. Ablation of UvrD1 sensitizes Mycobacterium smegmatis to killing by ultraviolet and ionizing radiation and to a single chromosomal break generated by I-SceI endonuclease. The physical and functional interactions of bacterial Ku and UvrD1 highlight the potential for cross-talk between components of nonhomologous end joining and nucleotide excision repair pathways.

Low density lipoprotein receptor gene Ava II polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

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Wu Dong-Feng

2011-02-01

Full Text Available Abstract Background Several common genetic polymorphisms in the low density lipoprotein receptor (LDL-R gene have associated with modifications of serum total cholesterol (TC and low density lipoprotein cholesterol (LDL-C levels, but the results are not consistent in different populations. Bai Ku Yao is a special subgroup of the Yao minority in China. The present study was undertaken to detect the association of LDL-R gene Ava Ⅱ polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations. Methods A total of 1024 subjects of Bai Ku Yao and 792 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples. Genotyping of the LDL-R gene Ava Ⅱ polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing. Results The levels of serum TC, high density lipoprotein cholesterol (HDL-C, LDL-C, apolipoprotein (Apo A1 and the ratio of ApoA1 to ApoB were lower in Bai Ku Yao than in Han (P - and A+ alleles was 65.5% and 34.5% in Bai Ku Yao, and 80.7% and 19.3% in Han (P -A-, A-A+ and A+A+ genotypes was 42.6%, 45.9% and 11.5% in Bai Ku Yao, and 64.9%, 31.6% and 3.5% in Han (P P 3.20 mmol/L subgroups in Bai Ku Yao (P P P P +A+ genotype had higher serum LDL-C, TC, HDL-C or ApoA1 levels than the subjects with A-A+ and A-A- genotypes. Spearman rank correlation analysis revealed that the levels of LDL-C in Bai Ku Yao and HDL-C in Han were correlated with genotypes (P P Conclusions The association of LDL-R gene Ava Ⅱ polymorphism and serum lipid levels is different between the Bai Ku Yao and Han populations. The discrepancy might partly result from different LDL-R gene Ava Ⅱ polymorphism or LDL-R gene-enviromental interactions.

DNA requirements for interaction of the C-terminal region of Ku80 with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs).

Science.gov (United States)

Radhakrishnan, Sarvan Kumar; Lees-Miller, Susan P

2017-09-01

Non-homologous end joining (NHEJ) is the major pathway for the repair of ionizing radiation induced DNA double strand breaks (DSBs) in human cells. Critical to NHEJ is the DNA-dependent interaction of the Ku70/80 heterodimer with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to form the DNA-PK holoenzyme. However, precisely how Ku recruits DNA-PKcs to DSBs ends to enhance its kinase activity has remained enigmatic, with contradictory findings reported in the literature. Here we address the role of the Ku80 C-terminal region (CTR) in the DNA-dependent interaction of Ku70/80 with DNA-PKcs using purified components and defined DNA structures. Our results show that the Ku80 CTR is required for interaction with DNA-PKcs on short segments of blunt ended 25bp dsDNA or 25bp dsDNA with a 15-base poly dA single stranded (ss) DNA extension, but this requirement is less stringent on longer dsDNA molecules (35bp blunt ended dsDNA) or 25bp duplex DNA with either a 15-base poly dT or poly dC ssDNA extension. Moreover, the DNA-PKcs-Ku complex preferentially forms on 25 bp DNA with a poly-pyrimidine ssDNA extension.Our work clarifies the role of the Ku80 CTR and dsDNA ends on the interaction of DNA-PKcs with Ku and provides key information to guide assembly and biology of NHEJ complexes. Copyright © 2017 Elsevier B.V. All rights reserved.

Absence of hemolytic disease of fetus and newborn despite maternal high-titer IgG anti-Ku.

Science.gov (United States)

Kakaiya, R M; Whaley, A; Howard-Menk, C; Rami, J; Papari, M; Campbell-Lee, S; Malecki, Z

2010-01-01

Anti-Ku seen in K(o) (Kell-null) individuals has previously been shown to cause severe hemolytic transfusion reactions. Maternal anti-Ku can cause none or moderate to severe hemolytic disease of the fetus and newborn (HDFN). In two of four previously described HDFN cases, intrauterine transfusions were required because of severe anemia. We report a case in which maternal anti-Ku did not cause HDFN. Standard serologic methods were used for RBC antibody screening and identification, adsorption and elution of RBC antibodies, and antigen typing. A gravida 3, para 3 (G3P3) woman was first evaluated in 2006 and was found to have an IgG RBC antibody that reacted against all panel RBCs in the anti-human globulin phase. A panel of RBCs treated with DTT did not react with the antibody. The antibody failed to react with one example of K(o) RBCs. The patient’s RBCs typed negative for the following Kell blood group antigens: KEL1, KEL2, KEL3, KEL4, KEL6, KEL7, KEL11, KEL13, and KEL18. These results established the presence of anti-Ku in maternal serum. The newborn was group A, D+ and required phototherapy for hyperbilirubinemia, but did not require transfusion. The woman was seen again in January 2010 during the third trimester (G4P3). At this time, anti-Ku titer was 256. She delivered a healthy group O, D+ baby boy at 37 weeks' gestation. Cord RBCs were 4+ for IgG by DAT. An eluate reacted with all RBCs tested, but did not react when tested against a panel of DTT-treated RBCs. K(o) phenotype is rare to begin with, and the maternal anti-Ku formation may require more than one pregnancy. Therefore, cases that can be evaluated for anti-Ku–related HDFN are rare. Our case contributes to serologic and clinical aspects of such rare cases.

Oxidative DNA Damage in Neurons: Implication of Ku in Neuronal Homeostasis and Survival

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Daniela De Zio

2012-01-01

Full Text Available Oxidative DNA damage is produced by reactive oxygen species (ROS which are generated by exogenous and endogenous sources and continuously challenge the cell. One of the most severe DNA lesions is the double-strand break (DSB, which is mainly repaired by nonhomologous end joining (NHEJ pathway in mammals. NHEJ directly joins the broken ends, without using the homologous template. Ku70/86 heterodimer, also known as Ku, is the first component of NHEJ as it directly binds DNA and recruits other NHEJ factors to promote the repair of the broken ends. Neurons are particularly metabolically active, displaying high rates of transcription and translation, which are associated with high metabolic and mitochondrial activity as well as oxygen consumption. In such a way, excessive oxygen radicals can be generated and constantly attack DNA, thereby producing several lesions. This condition, together with defective DNA repair systems, can lead to a high accumulation of DNA damage resulting in neurodegenerative processes and defects in neurodevelopment. In light of recent findings, in this paper, we will discuss the possible implication of Ku in neurodevelopment and in mediating the DNA repair dysfunction observed in certain neurodegenerations.

Superconducting magnet for a Ku-band maser.

Science.gov (United States)

Berwin, R.; Wiebe, E.; Dachel, P.

1972-01-01

A superconducting magnet to provide a uniform magnetic field of up to 8000 G in a 1.14-cm gap for the 15.3-GHz (Ku-band) traveling wave maser is described. The magnet operates in a persistent mode in the vacuum environment of a closed-cycle helium refrigerator (4.5 K). The features of a superconducting switch, which has both leads connected to 4.5 K heat stations and thereby does not receive heat generated by the magnet charging leads, are described.

DASCH ON KU Cyg: A ∼ 5 YEAR DUST ACCRETION EVENT IN ∼ 1900

International Nuclear Information System (INIS)

Tang Sumin; Grindlay, Jonathan; Los, Edward; Servillat, Mathieu

2011-01-01

KU Cyg is an eclipsing binary consisting of an F-type star accreting through a large accretion disk from a K5III red giant. Here we present the discovery of a 5 year dip around 1900 found from its 100 year DASCH light curve. It showed a ∼0.5 mag slow fading from 1899 to 1903 and brightened back around 1904 on a relatively shorter timescale. The light curve shape of the 1899-1904 fading-brightening event differs from the dust production and dispersion process observed in R Coronae Borealis stars, which usually has a faster fading and slower recovery, and for KU Cyg is probably related to the accretion disk surrounding the F star. The slow fading in KU Cyg is probably caused by increases in dust extinction in the disk, and the subsequent quick brightening may be due to the evaporation of dust transported inward through the disk. The extinction excess which caused the fading may arise from increased mass transfer rate in the system or from dust clump ejections from the K giant.

Characterization of the roles of the catalytic domains of Mycobacterium tuberculosis ligase D in Ku-dependent error-prone DNA end joining.

Science.gov (United States)

Wright, Douglas; DeBeaux, Austin; Shi, Runhua; Doherty, Aidan J; Harrison, Lynn

2010-09-01

We previously established an Escherichia coli strain capable of re-circularizing linear plasmid DNA by expressing the Mycobacterium tuberculosis Ku (Mt-Ku) and Mycobacterium tuberculosis ligase D (Mt-LigD) proteins from the E.coli chromosome. Repair was predominately mutagenic due to deletions at the termini. We hypothesized that these deletions could be due to a nuclease activity of Mt-LigD that was previously detected in vitro. Mt-LigD has three domains: an N-terminal polymerase domain (PolDom), a central domain with 3'-phosphoesterase and nuclease activity and a C-terminal ligase domain (LigDom). We generated bacterial strains expressing Mt-Ku and mutant versions of Mt-LigD. Plasmid re-circularization experiments in bacteria showed that the PolDom alone had no re-circularization activity. However, an increase in the total and accurate repair was found when the central domain was deleted. This provides further evidence that this central domain does have nuclease activity that can generate deletions during repair. Deletion of only the PolDom of Mt-LigD resulted in a complete loss of accurate repair and a significant reduction in total repair. This is in agreement with published in vitro work indicating that the PolDom is the major Mt-Ku-binding site. Interestingly, the LigDom alone was able to re-circularize plasmid DNA but only in an Mt-Ku-dependent manner, suggesting a potential second site for Ku-LigD interaction. This work has increased our understanding of the mutagenic repair by Mt-Ku and Mt-LigD and has extended the in vitro biochemical experiments by examining the importance of the Mt-LigD domains during repair in bacteria.

Oil Slick Observation at Low Incidence Angles in Ku-Band

Science.gov (United States)

Panfilova, M. A.; Karaev, V. Y.; Guo, Jie

2018-03-01

On the 20 April 2010 the oil platform Deep Water Horizon in the Gulf of Mexico suffered an explosion during the final phases of drilling an exploratory well. As a result, an oil film covered the sea surface area of several thousand square kilometers. In the present paper the data of the Ku-band Precipitation Radar, which operates at low incidence angles, were used to explore the oil spill event. The two-scale model of the scattering surface was used to describe radar backscatter from the sea surface. The algorithm for retrieval of normalized radar cross section at nadir and the total slope variance of large-scale waves compared to the wavelength of electromagnetic wave (22 mm) was developed for the Precipitation Radar swath. It is shown that measurements at low incidence angles can be used for oil spill detection. This is the first time that the dependence of mean square slope of large-scale waves on wind speed has been obtained for oil slicks from Ku-band data, and compared to mean square slope obtained by Cox and Munk from optical data.

Ku70 is required for late B cell development and immunoglobulin heavy chain class switching.

Science.gov (United States)

Manis, J P; Gu, Y; Lansford, R; Sonoda, E; Ferrini, R; Davidson, L; Rajewsky, K; Alt, F W

1998-06-15

Immunoglobulin (Ig) heavy chain (HC) class switch recombination (CSR) is a late B cell process that involves intrachromosomal DNA rearrangement. Ku70 and Ku80 form a DNA end-binding complex required for DNA double strand break repair and V(D)J recombination. Ku70(-/-) (K70T) mice, like recombination activating gene (RAG)-1- or RAG-2-deficient (R1T or R2T) mice, have impaired B and T cell development at an early progenitor stage, which is thought to result at least in part from defective V(D)J recombination (Gu, Y., K.J. Seidl, G.A. Rathbun, C. Zhu, J.P. Manis, N. van der Stoep, L. Davidson, H.L. Cheng, J.M. Sekiguchi, K. Frank, et al. 1997. Immunity. 7:653-665; Ouyang, H., A. Nussenzweig, A. Kurimasa, V.C. Soares, X. Li, C. Cordon-Cardo, W. Li, N. Cheong, M. Nussenzweig, G. Iliakis, et al. 1997. J. Exp. Med. 186:921-929). Therefore, to examine the potential role of Ku70 in CSR, we generated K70T mice that carry a germline Ig HC locus in which the JH region was replaced with a functionally rearranged VH(D)JH and Ig lambda light chain transgene (referred to as K70T/HL mice). Previously, we have shown that B cells from R1T or R2T mice carrying these rearranged Ig genes (R1T/HL or R2T/HL mice) can undergo CSR to IgG isotypes (Lansford, R., J. Manis, E. Sonoda, K. Rajewsky, and F. Alt. 1998. Int. Immunol. 10:325-332). K70T/HL mice had significant numbers of peripheral surface IgM+ B cells, which generated serum IgM levels similar to those of R2T/HL mice. However, in contrast to R2T/HL mice, K70T/HL mice had no detectable serum IgG isotypes. In vitro culture of K70T/HL B cells with agents that induce CSR in normal or R2T/HL B cells did lead to the induction of germline CH transcripts, indicating that initial signaling pathways for CSR were intact in K70T/HL cells. However, treatment with such agents did not lead to detectable CSR by K70T/HL B cells, and instead, led to cell death within 72 h. We conclude that Ku70 is required for the generation of B cells that have

'SON-GO-KU' : a dream of automated library

Science.gov (United States)

Sato, Mamoru; Kishimoto, Juji

In the process of automating libraries, the retrieval of books through the browsing of shelves is being overlooked. The telematic library is a document based DBMS which can deliver the content of books by simulating the browsing process. The retrieval actually simulates the process a person would use in selecting a book in a real library, where a visual presentation using a graphic display is substituted. The characteristics of prototype system "Son-Go-Ku" for such retrieval implemented in 1988 are mentioned.

Design of a Ku band miniature multiple beam klystron

Energy Technology Data Exchange (ETDEWEB)

Bandyopadhyay, Ayan Kumar, E-mail: ayan.bandyopadhyay@gmail.com; Pal, Debasish; Kant, Deepender [Microwave Tubes Division, CSIR-CEERI, Pilani, Rajasthan-333031 (India); Saini, Anil; Saha, Sukalyan; Joshi, Lalit Mohan

2016-03-09

The design of a miniature multiple beam klystron (MBK) working in the Ku-band frequency range is presented in this article. Starting from the main design parameters, design of the electron gun, the input and output couplers and radio frequency section (RF-section) are presented. The design methodology using state of the art commercial electromagnetic design tools, analytical formulae as well as noncommercial design tools are briefly presented in this article.

Analysis of metals in asphaltenes of KU-46 by PIXE analysis; Analisis de metales en asfaltenos de crudo mexicano KU-46 por PIXE

Energy Technology Data Exchange (ETDEWEB)

Navidad G, P.; Pina L, L.I.; Lopez M, J.; Ramirez T, J.J.; Aspiazu F, J.A.; Romero G, E.T. [Gerencia de Ciencias Basicas, Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

2002-07-01

The content of metals of the asphaltenes obtained from the KU-46 mexican crude with n-heptane was evaluated. The found metals in higher concentration are transition metals as well as the vanadium, nickel, copper and zinc. Moreover the sulfur in high concentrations was quantified. The metallic content of the asphaltenes revealed that the crude contains a lower quantity of metals unlike the vacuum residue previously analysed. (Author)

Analysis of a Compact Wideband Slotted Antenna for Ku Band Applications

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M. R. Ahsan

2014-01-01

Full Text Available The design procedure and physical module of a compact wideband patch antenna for Ku band application are presented in this paper. Finite element method based on 3D electromagnetic field solver has been utilized for the designing and analyzing process of proposed microstrip line fed modified E-H shaped electrically small patch antenna. After successful completion of the design process through various simulations, the proposed antenna has been fabricated on printed circuit board (PCB and its characteristics have been studied. The parameters of the proposed antenna prototype have been measured in standard far-field rectangular shape anechoic measurement compartment. It is apparent from the measured antenna parameters that the proposed antenna achieved almost stable variation of radiation pattern over the entire operational band with 1380 MHz of -10 dB return loss bandwidth. The maximum gain of 7.8 dBi and 89.97% average efficiency within the operating band from 17.15 GHz to 18.53 GHz ensure the suitability of the proposed antenna for Ku band applications.

Kuřim–the largest small town in the South-Moravian Region (Czechia)

Czech Academy of Sciences Publication Activity Database

Vaishar, Antonín

2015-01-01

Roč. 8, č. 8 (2015), s. 135-147 ISSN 2084-5456 Institutional support: RVO:68145535 Keywords : small town * suburbanization * Kuřim Subject RIV: DE - Earth Magnetism, Geodesy, Geography http://annalesgeo.up.krakow.pl/article/view/2839/2502

Synergistic effects of methyl methanesulfonate and X rays in inducing somatic mutations in the stamen hairs of Tradescantia clones, KU 27 and BNL 4430

International Nuclear Information System (INIS)

Ichikawa, Sadao; Yamaguchi, Akihiko; Okumura, Mikiko

1993-01-01

Young influorescences of Tradescantia clones KU 27 and BNL 4430, the both of which are blue/pink heterozygotes and have been demonstrated to be highly sensitive to alkylating agents, were exposed either to aqueous solutions of methyl methanesulfonate (MMS) for 16 hr alone (at 0.005 to 0.02% for KU 27 and at 0.005% for BNL 4430) or to acute 150 kVp X rays alone (161 to 531 mGy for Ku 27 and 501 to 976 mGy for BNL 4430), or in combinations (134 to 448 mGy for KU 27 and 458 to 865 mGy for BNL 4430 after the 0.005% MMS treatment). The induced somatic pink mutation frequencies per hair-cell division were studied and compared, and clone BNL 4430 was found to be nearly two times more sensitive to MMS than clone KU 27, while the X-ray-induced mutation frequencies in the latter was about 1.5 times higher than those in the former. The lower sensitivity to MMS of clone KU 27 (as compared with BNL 4430) was nevertheless about 5.6 times higher as compared with the responses of clone BNL 02 to MMS reported earlier, proving the high sensitivities of the two clones used in the present study. Clear synergistic effects of MMS and X rays were observed in the both clones, indicating that the mechanisms of inducing mutations are common at least in part between MMS and X rays. (author)

Vecāku pedagoģiskās kompetences pilnveide pirmsskolas izglītības iestāde

OpenAIRE

Vītola, Aija

2015-01-01

Maģistra darba autore Aija Vītola. Maģistra darba nosaukums „Vecāku pedagoģiskās kompetences pilnveide pirmsskolas izglītības iestādē”. Maģistra darba mērķis: izpētīt vecāku pedagoģiskās kompetences pilnveides iespējas pirmsskolas izglītības iestādē. Darba zinātniski teorētisko daļu veido: 1,5 – 3 gadus vecu bērnu personības attīstības likumsakarības, problēmas, ar kurām vecāki sastopas, bērnam uzsākot gaitas pirmsskolas izglītības iestādē, kompetences jēdziens, būtība, veidi, vecāku ...

Differential gene expression in a DNA double-strand-break repair mutant XRS-5 defective in Ku80. Analysis by cDNA microarray

International Nuclear Information System (INIS)

Chan, John Y.H.; Chen, Lung-Kun; Chang, Jui-Feng

2001-01-01

The ability of cells to rejoin DNA double-strand breaks (DSBs) usually correlates with their radiosensitivity. This correlation has been demonstrated in radiosensitive cells, including the Chinese hamster ovary mutant XRS-5. XRS-5 is defective in a DNA end-binding protein, Ku80, which is a component of a DNA-dependent protein kinase complex used for joining strand breaks. However, Ku80-deficient cells are known to be retarded in cell proliferation and growth as well as other yet to be identified defects. Using custom-made 600-gene cDNA microarray filters, we found differential gene expressions between the wild-type and XRS-5 cells. Defective Ku80 apparently affects the expression of several repair genes, including topoisomerase-I and -IIA, ERCC5, MLH1, and ATM. In contrast, other DNA repair-associated genes, such as GADD45A, EGR1 MDM2 and p53, were not affected. In addition, for large numbers of growth-associated genes, such as cyclins and clks, the growth factors and cytokines were also affected. Down-regulated expression was also found in several categories of seemingly unrelated genes, including apoptosis, angiogenesis, kinase and signaling, phosphatase, stress protein, proto-oncogenes and tumor suppressors, transcription and translation factors. A RT-PCR analysis confirmed that the XRS-5 cells used were defective in Ku80 expression. The diversified groups of genes being affected could mean that Ku80, a multi-functional DNA-binding protein, not only affects DNA repair, but is also involved in transcription regulation. Our data, taken together, indicate that there are specific genes being modulated in Ku80- deficient cells, and that some of the DNA repair pathways and other biological functions are apparently linked, suggesting that a defect in one gene could have global effects on many other processes. (author)

Differential gene expression in a DNA double-strand-break repair mutant XRS-5 defective in Ku80. Analysis by cDNA microarray

Energy Technology Data Exchange (ETDEWEB)

Chan, John Y.H.; Chen, Lung-Kun; Chang, Jui-Feng [National Yang Ming Univ., Taipei, Taiwan (China). Inst. of Radiological Sciences] (and others)

2001-12-01

The ability of cells to rejoin DNA double-strand breaks (DSBs) usually correlates with their radiosensitivity. This correlation has been demonstrated in radiosensitive cells, including the Chinese hamster ovary mutant XRS-5. XRS-5 is defective in a DNA end-binding protein, Ku80, which is a component of a DNA-dependent protein kinase complex used for joining strand breaks. However, Ku80-deficient cells are known to be retarded in cell proliferation and growth as well as other yet to be identified defects. Using custom-made 600-gene cDNA microarray filters, we found differential gene expressions between the wild-type and XRS-5 cells. Defective Ku80 apparently affects the expression of several repair genes, including topoisomerase-I and -IIA, ERCC5, MLH1, and ATM. In contrast, other DNA repair-associated genes, such as GADD45A, EGR1 MDM2 and p53, were not affected. In addition, for large numbers of growth-associated genes, such as cyclins and clks, the growth factors and cytokines were also affected. Down-regulated expression was also found in several categories of seemingly unrelated genes, including apoptosis, angiogenesis, kinase and signaling, phosphatase, stress protein, proto-oncogenes and tumor suppressors, transcription and translation factors. A RT-PCR analysis confirmed that the XRS-5 cells used were defective in Ku80 expression. The diversified groups of genes being affected could mean that Ku80, a multi-functional DNA-binding protein, not only affects DNA repair, but is also involved in transcription regulation. Our data, taken together, indicate that there are specific genes being modulated in Ku80- deficient cells, and that some of the DNA repair pathways and other biological functions are apparently linked, suggesting that a defect in one gene could have global effects on many other processes. (author)

The radiosensitizing effect of Ku70/80 knockdown in MCF10A cells irradiated with X-rays and p(66)+Be(40) neutrons.

Science.gov (United States)

Vandersickel, Veerle; Mancini, Monica; Slabbert, Jacobus; Marras, Emanuela; Thierens, Hubert; Perletti, Gianpaolo; Vral, Anne

2010-04-27

A better understanding of the underlying mechanisms of DNA repair after low- and high-LET radiations represents a research priority aimed at improving the outcome of clinical radiotherapy. To date however, our knowledge regarding the importance of DNA DSB repair proteins and mechanisms in the response of human cells to high-LET radiation, is far from being complete. We investigated the radiosensitizing effect after interfering with the DNA repair capacity in a human mammary epithelial cell line (MCF10A) by lentiviral-mediated RNA interference (RNAi) of the Ku70 protein, a key-element of the nonhomologous end-joining (NHEJ) pathway. Following irradiation of control and Ku-deficient cell lines with either 6 MV X-rays or p(66)+Be(40) neutrons, cellular radiosensitivity testing was performed using a crystal violet cell proliferation assay. Chromosomal radiosensitivity was evaluated using the micronucleus (MN) assay. RNAi of Ku70 caused downregulation of both the Ku70 and the Ku80 proteins. This downregulation sensitized cells to both X-rays and neutrons. Comparable dose modifying factors (DMFs) for X-rays and neutrons of 1.62 and 1.52 respectively were obtained with the cell proliferation assay, which points to the similar involvement of the Ku heterodimer in the cellular response to both types of radiation beams. After using the MN assay to evaluate chromosomal radiosensitivity, the obtained DMFs for X-ray doses of 2 and 4 Gy were 2.95 and 2.66 respectively. After neutron irradiation, the DMFs for doses of 1 and 2 Gy were 3.36 and 2.82 respectively. The fact that DMFs are in the same range for X-rays and neutrons confirms a similar importance of the NHEJ pathway and the Ku heterodimer for repairing DNA damage induced by both X-rays and p(66)+Be(40) neutrons. Interfering with the NHEJ pathway enhanced the radiosensitivity of human MCF10A cells to low-LET X-rays and high-LET neutrons, pointing to the importance of the Ku heterodimer for repairing damage induced by both

A novel coaxial Ku-band transit radiation oscillator without external guiding magnetic field

Energy Technology Data Exchange (ETDEWEB)

Ling, Junpu, E-mail: lingjunpu@163.com; Zhang, Jiande; He, Juntao; Jiang, Tao [College of Photoelectric Science and Engineering, National University of Defense Technology, Changsha 410073 (China)

2014-02-15

A novel coaxial transit radiation oscillator without external guiding magnetic field is designed to generate high power microwave at Ku-band. By using a coaxial structure, the space-charge potential energy is suppressed significantly, that is good for enhancing efficient beam-wave interaction. In order to improve the transmission stability of the unmagnetized intense relativistic electron beam, a Pierce-like cathode is employed in the novel device. By contrast with conventional relativistic microwave generators, this kind of device has the advantages of high stability, non-guiding magnetic field, and high efficiency. Moreover, with the coaxial design, it is possible to improve the power-handing capacity by increasing the radial dimension of the Ku-band device. With a 550 keV and 7.5 kA electron beam, a 1.25 GW microwave pulse at 12.08 GHz has been obtained in the simulation. The power conversion efficiency is about 30%.

PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF

Directory of Open Access Journals (Sweden)

Satish K. Tadi

2016-10-01

Full Text Available In mammalian cells, classical non-homologous end joining (c-NHEJ is critical for DNA double-strand break repair induced by ionizing radiation and during V(DJ recombination in developing B and T lymphocytes. Recently, PAXX was identified as a c-NHEJ core component. We report here that PAXX-deficient cells exhibit a cellular phenotype uncharacteristic of a deficiency in c-NHEJ core components. PAXX-deficient cells display normal sensitivity to radiomimetic drugs, are proficient in transient V(DJ recombination assays, and do not shift toward higher micro-homology usage in plasmid repair assays. Although PAXX-deficient cells lack c-NHEJ phenotypes, PAXX forms a stable ternary complex with Ku bound to DNA. Formation of this complex involves an interaction with Ku70 and requires a bare DNA extension for stability. Moreover, the relatively weak Ku-dependent stimulation of LIG4/XRCC4 activity by PAXX is unmasked by XLF ablation. Thus, PAXX plays an accessory role during c-NHEJ that is largely overlapped by XLF’s function.

Pirmsskolas vecuma bērnu vecāku emocionālais intelekts un stresa līmenis saistībā ar vecāku audzināšanas pieejām

OpenAIRE

Pastare, Ilze

2007-01-01

Rezumējums Pētījuma mērķis bija noskaidrot pirmsskolas vecuma bērnu vecāku emocionālā intelekta un stresa līmeņa rādītāju saistību ar audzināšanas pieejām. Kopā pētījumā piedalījās 41 māte. Vidējais vecums mātēm – 33 gadi, bērnu vidējais vecums – 5 gadi. Pētījumā tika izmantotas šādas metodes: “ Bar-On Emocionālā intelekta aptauja”, “Vecāku stresa indekss”, “ Alabamas audzināšanas aptaujas pirmsskolas versija”. Pētījumā tika noskaidrots, ka pastāv statistiski nozīmīga po...

Volba řešitelských strategií v závislosti na věku

Directory of Open Access Journals (Sweden)

Petr Eisenmann

2017-12-01

Full Text Available Příspěvek popisuje experiment, který byl součástí dlouhodobého výzkumu Rozvíjení kultury řešení matematických problémů ve školské praxi. Experiment byl proveden na vzorku celkem 584 respondentů ve věku 11–17 let a mapuje vývoj ve volbě řešitelských strategií matematických úloh u různých věkových skupin žáků. Žákům 6. a 8. ročníku základní školy, I. a III. ročníku střední školy byly předloženy tři úlohy, které se dají efektivně řešit pomocí heuristických strategií. Kromě úspěšnosti řešení úloh byla věnována pozornost také způsobu jejich řešení. Výsledkem experimentu je zjištění, že existují heuristické strategie, které žáci použili při řešení úloh i přesto, že s nimi ve výuce nebyli seznámeni. U některých z nich se jejich výskyt nemění v závislosti na věku.

Analysis of metals in asphaltenes of KU-46 by PIXE analysis

International Nuclear Information System (INIS)

Navidad G, P.; Pina L, L.I.; Lopez M, J.; Ramirez T, J.J.; Aspiazu F, J.A.; Romero G, E.T.

2002-01-01

The content of metals of the asphaltenes obtained from the KU-46 mexican crude with n-heptane was evaluated. The found metals in higher concentration are transition metals as well as the vanadium, nickel, copper and zinc. Moreover the sulfur in high concentrations was quantified. The metallic content of the asphaltenes revealed that the crude contains a lower quantity of metals unlike the vacuum residue previously analysed. (Author)

Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome

International Nuclear Information System (INIS)

Mimori, Tsuneyo; Ohosone, Yasuo; Hama, Nobuaki; Suwa, Akira; Akizuki, Masashi; Homma, Mitsuo; Griffith, A.J.; Hardin, J.A.

1990-01-01

Anti-Ku (p70/p80) autoantibodies in patients with scleroderma-polymyositis overlap syndrome recognize a 70-kDa/80-kDa protein heterodimer which binds to terminal regions of double-stranded DNA. In the present study, the authors isolated full-length cDNAs that encode the 80-kDa Ku subunit. Initial screening of a human spleen cDNA library with anti-Ku antibodies yielded a cDNA of 1.0 kilobase (kb) (termed K71) encoding a portion of the 80-kDa Ku polypeptide (identification based on immunological criteria). In RNA blots, this cDNA hybridized with two mRNAs of 3.4 and 2.6 kb. In vitro transcription and translation experiments produced an immunoprecipitable polypeptide which comigrated with the 80-kDa Ku subunit. The Ku80-6 cDNA proved to be 3304 nucleotides in length, with an additional poly(A) tail, closely approximating the size of the larger mRNA. It contains a single long open reading frame encoding 732 amino acids. The putative polypeptide has a high content of acidic amino acids and a region with periodic repeat of leucine in every seventh position which may form the leucine zipper structure. In genomic DNA blots, probes derived from the opposite ends of cDNA Ku80-6 hybridized with several nonoverlapping restriction fragments from human leukocyte DNA, indicating that the gene encoding the 80-kDa Ku polypeptide is divided into several exons by intervening sequences

Renewal of KU-FEL Facility

CERN Document Server

Kii, Toshiteru; Masuda, Kai; Murakami, Shio; Ohgaki, Hideaki; Yamazaki, Tetsuo; Yoshikawa, Kiyoshi; Zen, Heishun

2004-01-01

Users demands to a high power tunable IR laser are increasing in Japan in energy-related science, such as basic study of high-efficiency solar cells, generation of new energy source of alcohol and/or H2 from polluted gas, and separation of DNA and/or RNA. To satisfy these demands, we decided to renew our FEL facility more user friendly and to operate more flexibly. Construction and fundamental studies on the KU-FEL have been carried out at a building of Institute of Chemical Research where few other accelerators are operating. Therefore, available machine time for our experiments is quite limited. We are now modifying the room by adding concrete walls of 2-m thickness and some space for users will be available. The present FEL system will be moved to the room A photocathode RF-gun system will be nearly added to the system and the present thermionic RF-gun will be used ternatively according to the demands of users. The photocathode material will be Cs2Te. The room with the shielding will be completed in June, ...

Experimental demonstration of a Ku-band radial-line relativistic klystron oscillator based on transition radiation

Science.gov (United States)

Dang, Fangchao; Zhang, Xiaoping; Zhang, Jun; Ju, Jinchuan; Zhong, Huihuang

2017-03-01

We report on a radial-line relativistic klystron oscillator (RL-RKO), which is physically designed to generate gigawatt-level high power microwaves (HPMs) at Ku-band. The 3π/4 mode of a four-gap buncher is selected to highly modulate the radially propagating intense relativistic electron beam (IREB). A three-gap extractor operating at the π mode is employed to extract the radio-frequency energy efficiently. The Ku-band RL-RKO is investigated experimentally on an intense-current electron beam accelerator. The radially propagating IREB is well focused with an axial-width of 2 mm by a radial magnetic field of 0.4 T. Microwaves with a frequency of 14.86 GHz and a power of 1.5 GW are generated, corresponding to an efficiency of 24%, which indicates a significant advance for the research of radial-line HPM sources.

[Professor Wacław Kuśnierczyk (1908-1997)--Pro Memoria in the century of birthday].

Science.gov (United States)

Brozek, Krzysztof; Kozakiewicz, Jacek; Kierzek, Andrzej

2009-01-01

Wacław Kuśnierczyk was born in 1908 in Sniatyń. He received the degree in medicine at Jan Kazimierz University in Lwów in 1932. He did his PhD degree under Professor Zaleski supervision in 1938 at Jan Kazimierz University. At that time he concentrated his scientific activity on research on tuberculosis. In 1953 he obtained the title of second degree specialist in ear, nose and throat diseases. He became a chief of Otolaryngology at Urban Hospital No 4 in Katowice in 1960. Since then this eminent physician was working on tumours located in upper respiratory tract and the possibility of its endoscopic diagnosis at Silesian Academy of Medicine in Katowice. As one of the first he pointed out the negative influence of smoking cigarettes on cancer of larynx. It was Wacław Kuśnierczyk who implemented new priorities for integrated programs in patient care, research, education and cancer prevention. He has published widely in peer reviewed journals and has edited or contributed to many books. He has given many major lectures and is the recipient of numerous prestigious awards for his scientific accomplishments. The achievement of Professor Kuśnierczyk were the valuable source of information for the physicians. In 1997, on the 31st of January he died in Katowice.

Effect of Ku80 deficiency on mutation frequencies and spectra at a LacZ reporter locus in mouse tissues and cells.

Directory of Open Access Journals (Sweden)

Rita A Busuttil

Full Text Available Non-homologous end joining (NHEJ is thought to be an important mechanism for preventing the adverse effects of DNA double strand breaks (DSBs and its absence has been associated with premature aging. To investigate the effect of inactivated NHEJ on spontaneous mutation frequencies and spectra in vivo and in cultured cells, we crossed a Ku80-deficient mouse with mice harboring a lacZ-plasmid-based mutation reporter. We analyzed various organs and tissues, as well as cultured embryonic fibroblasts, for mutations at the lacZ locus. When comparing mutant with wild-type mice, we observed a significantly higher number of genome rearrangements in liver and spleen and a significantly lower number of point mutations in liver and brain. The reduced point mutation frequency was not due to a decrease in small deletion mutations thought to be a hallmark of NHEJ, but could be a consequence of increased cellular responses to unrepaired DSBs. Indeed, we found a substantial increase in persistent 53BP1 and gammaH2AX DNA damage foci in Ku80-/- as compared to wild-type liver. Treatment of cultured Ku80-deficient or wild-type embryonic fibroblasts, either proliferating or quiescent, with hydrogen peroxide or bleomycin showed no differences in the number or type of induced genome rearrangements. However, after such treatment, Ku80-deficient cells did show an increased number of persistent DNA damage foci. These results indicate that Ku80-dependent repair of DNA damage is predominantly error-free with the effect of alternative more error-prone pathways creating genome rearrangements only detectable after extended periods of time, i.e., in young adult animals. The observed premature aging likely results from a combination of increased cellular senescence and an increased load of stable, genome rearrangements.

Ku-band electromagnetic wave absorbing properties of polysiloxane derived Si-O-C bulk ceramics

Science.gov (United States)

Ding, Donghai; Li, Zipei; Xiao, Guoqing; Yang, Shaoyu

2018-02-01

The bulk Si-O-C ceramics were prepared by polymer derived ceramics (PDCs) route using polysiloxane as precursor and their properties were investigated for electromagnetic wave absorbing in the frequency range of 12.4-18 GHz (Ku-band). It was found that the catalytic pyrolysis can enhance substantially the absorbing properties by in situ formation of turbostratic carbon network, ordered carbon, and multi-wall carbon nanotubes. The matching thickness of sample containing 1.5 wt% FeCl3 (FPSO-1.5) is 2.2 mm, and its reflection loss exceeds -10 dB in the whole Ku-band with an absorption peak of -35.48 dB at 14.16 GHz. For sample containing 1.5 wt% FeCl3, its absorption peak increases to -15.78 dB, but its matching thickness decreases significantly to 2.2 mm. The polymer derived Si-O-C ceramics could be used as excellent electromagnetic functional devices working in harsh environments.

Suppression of the asymmetric competition mode in the relativistic Ku-band coaxial transit-time oscillator

Energy Technology Data Exchange (ETDEWEB)

Ling, Junpu; He, Juntao; Zhang, Jiande; Jiang, Tao; Wang, Lei [College of Optoelectronic Science and Engineering, National University of Defense Technology, Changsha 410073 (China)

2014-10-15

A relativistic Ku-band coaxial transit-time oscillator has been proposed in our previous work. In the experiments, we find that the asymmetric competition mode in the device limits the microwave power with the increase of the input electric power. For solving such a problem, the methods for analysis and suppression of the asymmetric competition mode in the device are investigated theoretically and experimentally. It is shown that the structure and the material of the collector, the concentricity, and the electron emission uniformity play an important part in the suppression of the asymmetric competition mode in the relativistic Ku-band transit-time oscillator. In the subsequent experiments, the asymmetric mode was suppressed effectively. At a low guiding magnetic field of 0.7 T, a microwave pulse with power of 1 GW, frequency of 14.3 GHz close to the simulation one, and efficiency of 20% was generated.

Expression of Mycobacterium tuberculosis Ku and Ligase D in Escherichia coli results in RecA and RecB-independent DNA end-joining at regions of microhomology.

Science.gov (United States)

Malyarchuk, Svitlana; Wright, Douglas; Castore, Reneau; Klepper, Emily; Weiss, Bernard; Doherty, Aidan J; Harrison, Lynn

2007-10-01

Unlike Escherichia coli, Mycobacterium tuberculosis (Mt) expresses a Ku-like protein and an ATP-dependent DNA ligase that can perform non-homologous end-joining (NHEJ). We have expressed the Mt-Ku and Mt-Ligase D in E. coli using an arabinose-inducible promoter and expression vectors that integrate into specific sites in the E. coli chromosome. E. coli strains have been generated that express the Mt-Ku and Mt-Ligase D on a genetic background that is wild-type for repair, or deficient in either the RecA or RecB protein. Transformation of these strains with linearized plasmid DNA containing a 2bp overhang has demonstrated that expression of both the Mt-Ku and Mt-Ligase D is required for DNA end-joining and that loss of RecA does not prevent this double-strand break repair. Analysis of the re-joined plasmid has shown that repair is predominantly inaccurate and results in the deletion of sequences. Loss of RecB did not prevent the formation of large deletions, but did increase the amount of end-joining. Sequencing the junctions has revealed that the majority of the ligations occurred at regions of microhomology (1-4bps), eliminating one copy of the homologous sequence at the junction. The Mt-Ku and Mt-Ligase D can therefore function in E. coli to re-circularize linear plasmid.

Enterocin X, a novel two-peptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides.

Science.gov (United States)

Hu, Chih-Bo; Malaphan, Wanna; Zendo, Takeshi; Nakayama, Jiro; Sonomoto, Kenji

2010-07-01

Enterocin X, composed of two antibacterial peptides (Xalpha and Xbeta), is a novel class IIb bacteriocin from Enterococcus faecium KU-B5. When combined, Xalpha and Xbeta display variably enhanced or reduced antibacterial activity toward a panel of indicators compared to each peptide individually. In E. faecium strains that produce enterocins A and B, such as KU-B5, only one additional bacteriocin had previously been known.

Initial assessment of an airborne Ku-band polarimetric SAR.

Energy Technology Data Exchange (ETDEWEB)

Raynal, Ann Marie; Doerry, Armin Walter

2013-02-01

Polarimetric synthetic aperture radar (SAR) has been used for a variety of dual-use research applications since the 1940s. By measuring the direction of the electric field vector from radar echoes, polarimetry may enhance an analysts understanding of scattering effects for both earth monitoring and tactical surveillance missions. Polarimetry may provide insight into surface types, materials, or orientations for natural and man-made targets. Polarimetric measurements may also be used to enhance the contrast between scattering surfaces such as man-made objects and their surroundings. This report represents an initial assessment of the utility of, and applications for, polarimetric SAR at Ku-band for airborne or unmanned aerial systems.

Izrada 3D modela kućišta centrifugalne pumpe

OpenAIRE

Hršak, Božidar; Golubić, Stjepan; Carek, Dalibor

2012-01-01

Prikazana je izrada 3D modela kućišta centrifugalne pumpe na temelju postojećeg 2D nacrta gotovog proizvoda primjenom sinkrone tehnologije 3D modeliranja, i reverzibilnog inženjeringa. Na temelju kreiranog 3D modela, predložene su moguće modifikacije postojeće izvedbe proizvoda. Provedene su analize zakrivljenosti površina, Bézierove krivulje modela i 3D geometrije. Prikazana je i mogućnost vizualizacije gotovog 3D modela na Web stranicama u cilju prezentiranja proizvoda budućim kupcima primj...

Enterocin X, a Novel Two-Peptide Bacteriocin from Enterococcus faecium KU-B5, Has an Antibacterial Spectrum Entirely Different from Those of Its Component Peptides▿

Science.gov (United States)

Hu, Chih-Bo; Malaphan, Wanna; Zendo, Takeshi; Nakayama, Jiro; Sonomoto, Kenji

2010-01-01

Enterocin X, composed of two antibacterial peptides (Xα and Xβ), is a novel class IIb bacteriocin from Enterococcus faecium KU-B5. When combined, Xα and Xβ display variably enhanced or reduced antibacterial activity toward a panel of indicators compared to each peptide individually. In E. faecium strains that produce enterocins A and B, such as KU-B5, only one additional bacteriocin had previously been known. PMID:20418437

EPR study of gamma and neutron irradiation effects on KU1, KS-4V and Infrasil 301 silica glasses

International Nuclear Information System (INIS)

Lagomacini, Juan C.; Bravo, David; Leon, Monica; Martin, Piedad; Ibarra, Angel; Martin, Agustin; Lopez, Fernando J.

2011-01-01

Electron paramagnetic resonance (EPR) studies have been carried out on KU1 and KS-4V high purity quartz glasses and commercial silica Infrasil 301, irradiated with gamma rays up to a dose of 11.6 MGy and neutron fluences of 10 21 and 10 22 n/m 2 . Gamma irradiations produce a much higher concentration of defect centres (mainly E', POR and NBOHC) for KU1 and I301 than for KS-4V silica. In contrast, neutron irradiation at the highest fluence produces similar concentrations in all silica types. These results agree to a good extent with those obtained in previous optical absorption measurements. Moreover, oxygen-related centres (POR and NBOHC) have been well characterized by means of electron paramagnetic resonance.

EPR study of gamma and neutron irradiation effects on KU1, KS-4V and Infrasil 301 silica glasses

Energy Technology Data Exchange (ETDEWEB)

Lagomacini, Juan C., E-mail: jc.lagomacini@uam.es [Dept. Fisica de Materiales, Universidad Autonoma de Madrid, E-28049 Madrid (Spain); Bravo, David [Dept. Fisica de Materiales, Universidad Autonoma de Madrid, E-28049 Madrid (Spain); Leon, Monica; Martin, Piedad; Ibarra, Angel [Materiales para Fusion, CIEMAT, Avda. Complutense 22, E-28040 Madrid (Spain); Martin, Agustin [Dept. Fisica e Instalaciones, ETS Arquitectura UPM, E-28040 Madrid (Spain); Lopez, Fernando J. [Dept. Fisica de Materiales, Universidad Autonoma de Madrid, E-28049 Madrid (Spain)

2011-10-01

Electron paramagnetic resonance (EPR) studies have been carried out on KU1 and KS-4V high purity quartz glasses and commercial silica Infrasil 301, irradiated with gamma rays up to a dose of 11.6 MGy and neutron fluences of 10{sup 21} and 10{sup 22} n/m{sup 2}. Gamma irradiations produce a much higher concentration of defect centres (mainly E', POR and NBOHC) for KU1 and I301 than for KS-4V silica. In contrast, neutron irradiation at the highest fluence produces similar concentrations in all silica types. These results agree to a good extent with those obtained in previous optical absorption measurements. Moreover, oxygen-related centres (POR and NBOHC) have been well characterized by means of electron paramagnetic resonance.

The Shock and Vibration Digest. Volume 16, Number 6

Science.gov (United States)

1984-06-01

Meuurement of Rigid Body Modes for Dynamic Deagn N. Okuboand T. Furukawa CAMAL Chuo Univ., Tokyo, Japan, Intl. Modal Analysis Conf., Proc. 2nd, Orlando... CAMAL , Chuo Univ., Tokyo, Japan, Intl. VV :ä\\ Analysis Conf., Proc. 2nd, Orlando, FL, Feh ö-Q. 1984, Spons. by Union College

Comparative Assessment of Women Farmers' Status in Japan and ...

African Journals Online (AJOL)

The research compared the status of Japanese and Nigerian women farmers. The study was conducted in Sera Chuo, Hiroshima and Ohnan Chuo, Shimane prefecture, Chugoku region of Japan and Southwestern part of Nigeria covering three locations (Omi-Adio, Ijaye and Kila) within the suburban area of Ibadan ...

Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70.

Science.gov (United States)

Boboila, Cristian; Jankovic, Mila; Yan, Catherine T; Wang, Jing H; Wesemann, Duane R; Zhang, Tingting; Fazeli, Alex; Feldman, Lauren; Nussenzweig, Andre; Nussenzweig, Michel; Alt, Frederick W

2010-02-16

Class switch recombination (CSR) in B lymphocytes is initiated by introduction of multiple DNA double-strand breaks (DSBs) into switch (S) regions that flank immunoglobulin heavy chain (IgH) constant region exons. CSR is completed by joining a DSB in the donor S mu to a DSB in a downstream acceptor S region (e.g., S gamma1) by end-joining. In normal cells, many CSR junctions are mediated by classical nonhomologous end-joining (C-NHEJ), which employs the Ku70/80 complex for DSB recognition and XRCC4/DNA ligase 4 for ligation. Alternative end-joining (A-EJ) mediates CSR, at reduced levels, in the absence of C-NHEJ, even in combined absence of Ku70 and ligase 4, demonstrating an A-EJ pathway totally distinct from C-NHEJ. Multiple DSBs are introduced into S mu during CSR, with some being rejoined or joined to each other to generate internal switch deletions (ISDs). In addition, S-region DSBs can be joined to other chromosomes to generate translocations, the level of which is increased by absence of a single C-NHEJ component (e.g., XRCC4). We asked whether ISD and S-region translocations occur in the complete absence of C-NHEJ (e.g., in Ku70/ligase 4 double-deficient B cells). We found, unexpectedly, that B-cell activation for CSR generates substantial ISD in both S mu and S gamma1 and that ISD in both is greatly increased by the absence of C-NHEJ. IgH chromosomal translocations to the c-myc oncogene also are augmented in the combined absence of Ku70 and ligase 4. We discuss the implications of these findings for A-EJ in normal and abnormal DSB repair.

A wideband superconducting filter at Ku-band based on interdigital coupling

Science.gov (United States)

Jiang, Ying; Wei, Bin; Cao, Bisong; Li, Qirong; Guo, Xubo; Jiang, Linan; Song, Xiaoke; Wang, Xiang

2018-04-01

In this paper, an interdigital-type resonator with strong electric coupling is proposed for the wideband high-frequency (>10 GHz) filter design. The proposed microstrip resonator consists of an H-shaped main line part with its both ends installed with interdigital finger parts. Strong electric coupling is achieved between adjacent resonators. A six-pole high-temperature superconducting filter at Ku-band using this resonator is designed and fabricated. The filter has a center frequency of 15.11 GHz with a fractional bandwidth of 30%. The insertion loss of the passband is less than 0.3 dB, and the return loss is greater than 14 dB without any tuning.

A creative approach to the development of an agenda for knowledge utilization: outputs from the 11th international knowledge utilization colloquium (KU 11).

Science.gov (United States)

Wilkinson, Joyce E; Rycroft-Malone, Jo; Davies, Huw T O; McCormack, Brendan

2012-12-01

A group of researchers and practitioners interested in advancing knowledge utilization met as a colloquium in Belfast (KU 11) and used a "world café" approach to exploit the social capital and shared understanding built up over previous events to consider the research and practice agenda. We considered three key areas of relevance to knowledge use: (1) understanding the nature of research use, influence and impact; (2) blended and collaborative approaches to knowledge production and use; and (3) supporting sustainability and spread of evidence-informed innovations. The approach enabled the development of artifacts that reflected the three areas and these were analyzed using a creative hermeneutic approach. The themes that emerged and which are outlined in this commentary are not mutually exclusive. There was much overlap in the discussions and therefore of the themes, reflecting the complex nature of knowledge translation work. The agenda that has emerged from KU 11 also reflects the participatory and creative approach in which the meeting was structured and focused, and therefore emphasizes the processual, relational and contingent nature of some of the challenges we face. The past 20 years has seen an explosion in activity around understanding KU, and we have learned much about the difficulties. Whilst the agenda for the next decade may be becoming clearer, colloquia such as KU 11, using creative and engaging approaches, have a key role to play in dissecting, articulating and sharing that agenda. In this way, we also build an ever-expanding international community that is dedicated to working towards increasing the chances of success for better patient care. © 2012 Sigma Theta Tau International.

Study of sea-surface slope distribution and its effect on radar backscatter based on Global Precipitation Measurement Ku-band precipitation radar measurements

Science.gov (United States)

Yan, Qiushuang; Zhang, Jie; Fan, Chenqing; Wang, Jing; Meng, Junmin

2018-01-01

The collocated normalized radar backscattering cross-section measurements from the Global Precipitation Measurement (GPM) Ku-band precipitation radar (KuPR) and the winds from the moored buoys are used to study the effect of different sea-surface slope probability density functions (PDFs), including the Gaussian PDF, the Gram-Charlier PDF, and the Liu PDF, on the geometrical optics (GO) model predictions of the radar backscatter at low incidence angles (0 deg to 18 deg) at different sea states. First, the peakedness coefficient in the Liu distribution is determined using the collocations at the normal incidence angle, and the results indicate that the peakedness coefficient is a nonlinear function of the wind speed. Then, the performance of the modified Liu distribution, i.e., Liu distribution using the obtained peakedness coefficient estimate; the Gaussian distribution; and the Gram-Charlier distribution is analyzed. The results show that the GO model predictions with the modified Liu distribution agree best with the KuPR measurements, followed by the predictions with the Gaussian distribution, while the predictions with the Gram-Charlier distribution have larger differences as the total or the slick filtered, not the radar filtered, probability density is included in the distribution. The best-performing distribution changes with incidence angle and changes with wind speed.

Wpływ wysiłku fizycznego na funkcję układu oddechowego w twardzinie układowej

Directory of Open Access Journals (Sweden)

Stanisław Sierakowski

2010-12-01

Full Text Available Cel: Powikłania ze strony układu oddechowego stanowią główną przyczynę zgonów pacjentów z twardziną układową (TU. Tętniczenadciśnienie płucne i śródmiąższowa choroba płuc stanowią najistotniejszez nich. Pacjenci są jednak często zdiagnozowani dopierow późnym okresie choroby, dlatego też istotne jest opracowaniemetody diagnostycznej do wczesnego wykrywania tych powikłań.Celem pracy była ocena wpływu wysiłku fizycznego na zmiany parametrówoddechowych. Materiały i metody: Badania przeprowadzono wśród 32 pacjentów zezdiagnozowaną TU; 72% pacjentów miało postać ograniczoną TU,28% postać uogólnioną. Wykonywano pomiar zdolności dyfuzyjnejpłuc dla tlenku węgla (DLCO, natężonej pojemności życiowej (FVC,natężonej objętości wydechowej pierwszosekundowej (FEV1 orazcałkowitej pojemności płuc (TLC w spoczynku oraz bezpośrednio pozakończeniu wysiłku fizycznego. Wyniki: Pod wpływem wysiłku fizycznego obserwowano wzrostDLCO od 59,7% do 68,1% wartości należnej (p < 0,005. Nie stwierdzononatomiast istotnych statystycznie zmian wartości FVC, FEV1oraz TLC. Wnioski: U chorych na TU zdolność dyfuzyjna płuc dla tlenku węglapo wysiłku fizycznym uległa istotnej statystycznie poprawiew porównaniu z wartościami spoczynkowymi. Związane jest toz koniecznością zapewnienia właściwego utlenowania krwi tętniczeji wynika m.in. ze zwiększenia pojemności łożyska naczyniowego.W klinicznej interpretacji wartości DLCO należy uwzględniać opróczstężenia hemoglobiny i objętości pęcherzykowej także wpływ wysiłkuoraz związane z nim zmiany wielkości objętości rzutu serca.

Evaluation of genetic alteration induced by radon gas using the micronucleus test (Tradescantia sp. clone KU-20)

International Nuclear Information System (INIS)

Bruschi, Armando L.; Azevedo, Heliana de; Macacini, Jose F.; Roque, Claudio V.

2011-01-01

The first observations over the existence of radon gas (Rn), initially known as 'thorium emanation', were carried out between the end of 19 th and beginning of 20 th centuries. A result of uranium-238 (U 238 ) radioactive decay, radon is a tasteless, odorless and colorless gas under room temperature, with a 3.825-day half life and particle α emission in its decay, and as final product of its disintegration, the stable lead-206 isotope (Pb 206 ). Being it is the gas with the highest density known, closed and poor ventilated environments are favorable to its accumulation, with its inhalation being the highest health risk. The use of vegetal bioindicators has shown to be excellent on the monitoring of air quality and on mutagenic potential of various pollutants contained in the atmosphere. Within this context, the objective of this study was to evaluate the micronucleus test application potential utilizing the Tradescantia sp. clone KU-20, in order to evaluate genetic alterations induced by radon gas. Stems of Tradescantia sp. clone KU-20, previously immerse in Hoagland solution, were introduced in a radon detection equipment's calibration chamber (Alphaguard), containing radium salt. Afterwards, the accommodated stems were exposed to radon gas (the average radon concentration was 7.639 KBq/m3) for 24 hours. The results demonstrated an increase on micronucleus formation (39.23 + 2.143 MCN/100 tetrads) in stems exposed in relation to the negative control (18.00 + 1.396 MCN/100 tetrads). The difference between the values indicated a significant increase on micronucleus frequency in the inflorescences subjected to radon gas. The presented results demonstrated the micronucleus test application potential using Tradescantia clone KU-20 to evaluate genetic effects induced by radon gas. (author)

Improved foilless Ku-band transit-time oscillator for generating gigawatt level microwave with low guiding magnetic field

Energy Technology Data Exchange (ETDEWEB)

Ling, Junpu; He, Juntao, E-mail: hejuntao12@163.com; Zhang, Jiande; Jiang, Tao; Hu, Yi [College of Optoelectronic Science and Engineering, National University of Defense Technology, Changsha 410073 (China)

2014-09-15

An improved foilless Ku-band transit-time oscillator with low guiding magnetic field is proposed and investigated in this paper. With a non-uniform buncher and a coaxial TM{sub 02} mode dual-resonant reflector, this improved device can output gigawatt level Ku-band microwave with relatively compact radial dimensions. Besides the above virtue, this novel reflector also has the merits of high TEM reflectance, being more suitable for pre-modulating the electron beam and enhancing the conversion efficiency. Moreover, in order to further increase the conversion efficiency and lower the power saturation time, a depth-tunable coaxial collector and a resonant cavity located before the extractor are employed in our device. Main structure parameters of the device are optimized by particle in cell simulations. The typical simulation result is that, with a 380 kV, 8.2 kA beam guided by a magnetic field of about 0.6 T, 1.15 GW microwave pulse at 14.25 GHz is generated, yielding a conversion efficiency of about 37%.

Apigenin induces apoptosis by targeting inhibitor of apoptosis proteins and Ku70–Bax interaction in prostate cancer

Science.gov (United States)

Shukla, Sanjeev; Fu, Pingfu; Gupta, Sanjay

2014-01-01

Dysfunction of the apoptotic pathway in prostate cancer cells confers apoptosis resistance towards various therapies. A novel strategy to overcome resistance is to directly target the apoptotic pathway in cancer cells. Apigenin, an anticancer agent, selectively toxic to cancer cells induces cell cycle arrest and apoptosis through mechanisms which are not fully explored. In the present study we provide novel insight into the mechanisms of apoptosis induction by apigenin. Treatment of androgen-refractory human prostate cancer PC-3 and DU145 cells with apigenin resulted in dose-dependent suppression of XIAP, c-IAP1, c-IAP2 and survivin protein levels. Apigenin treatment resulted in significant decrease in cell viability and apoptosis induction with the increase of cytochrome C in time-dependent manner. These effects of apigenin were accompanied by decrease in Bcl-xL and Bcl-2 and increase in the active form of Bax protein. The apigenin-mediated increase in Bax was due to dissociation of Bax from Ku70 which is essential for apoptotic activity of Bax. Apigenin treatment resulted in the inhibition of class I histone deacetylases and HDAC1 protein expression, thereby increasing the acetylation of Ku70 and the dissociation of Bax resulting in apoptosis of cancer cells. Furthermore, apigenin significantly reduced HDAC1 occupancy at the XIAP promoter, suggesting that histone deacetylation might be critical for XIAP downregulation. These results suggest that apigenin targets inhibitor of apoptosis proteins and Ku70–Bax interaction in the induction of apoptosis in prostate cancer cells and in athymic nude mouse xenograft model endorsing its in vivo efficacy. PMID:24563225

Rapid Recruitment of BRCA1 to DNA Double-Strand Breaks Is Dependent on Its Association with Ku80▿ †

Science.gov (United States)

Wei, Leizhen; Lan, Li; Hong, Zehui; Yasui, Akira; Ishioka, Chikashi; Chiba, Natsuko

2008-01-01

BRCA1 is the first susceptibility gene to be linked to breast and ovarian cancers. Although mounting evidence has indicated that BRCA1 participates in DNA double-strand break (DSB) repair pathways, its precise mechanism is still unclear. Here, we analyzed the in situ response of BRCA1 at DSBs produced by laser microirradiation. The amino (N)- and carboxyl (C)-terminal fragments of BRCA1 accumulated independently at DSBs with distinct kinetics. The N-terminal BRCA1 fragment accumulated immediately after laser irradiation at DSBs and dissociated rapidly. In contrast, the C-terminal fragment of BRCA1 accumulated more slowly at DSBs but remained at the sites. Interestingly, rapid accumulation of the BRCA1 N terminus, but not the C terminus, at DSBs depended on Ku80, which functions in the nonhomologous end-joining (NHEJ) pathway, independently of BARD1, which binds to the N terminus of BRCA1. Two small regions in the N terminus of BRCA1 independently accumulated at DSBs and interacted with Ku80. Missense mutations found within the N terminus of BRCA1 in cancers significantly changed the kinetics of its accumulation at DSBs. A P142H mutant failed to associate with Ku80 and restore resistance to irradiation in BRCA1-deficient cells. These might provide a molecular basis of the involvement of BRCA1 in the NHEJ pathway of the DSB repair process. PMID:18936166

Izrada 3D modela kućišta ravnog ventila DN 50

OpenAIRE

Hršak, Božidar; Golubić, Stjepan; Bošnjak, Matej

2012-01-01

Prikazana je izrada 3D modela kućišta centrifugalne pumpe na temelju postojećeg 2D nacrta gotovog proizvoda primjenom sinkrone tehnologije 3D modeliranja, i reverzibilnog inženjeringa. Na temelju kreiranog 3D modela, predložene su moguće modifikacije postojeće izvedbe proizvoda. Provedene su analize zakrivljenosti površina, Bézierove krivulje modela i 3D geometrije. Prikazana je i mogućnost vizualizacije gotovog 3D modela na Web stranicama u cilju prezentiranja proizvoda budućim kupcima primj...

Shuttle Ku-band bent-pipe implementation considerations. [for Space Shuttle digital communication systems

Science.gov (United States)

Batson, B. H.; Seyl, J. W.; Huth, G. K.

1977-01-01

This paper describes an approach for relay of data-modulated subcarriers from Shuttle payloads through the Shuttle Ku-band communications subsystem (and subsequently through a tracking and data relay satellite system to a ground terminal). The novelty is that a channel originally provided for baseband digital data is shown to be suitable for this purpose; the resulting transmission scheme is referred to as a narrowband bent-pipe scheme. Test results demonstrating the validity of the narrowband bent-pipe mode are presented, and limitations on system performance are described.

Detailed modeling of local anisotropy and transverse Ku interplay regarding hysteresis loop in FeCuNbSiB nanocrystalline ribbons

Science.gov (United States)

Geoffroy, Olivier; Boust, Nicolas; Chazal, Hervé; Flury, Sébastien; Roudet, James

2018-04-01

This article focuses on the modeling of the hysteresis loop featured by Fe-Cu-Nb-Si-B nanocrystalline alloys with transverse induced anisotropy. The magnetization reversal process of a magnetic correlated volume (CV), characterized by the induced anisotropy Ku, and a deviation of the local easy magnetization direction featuring the effect of a local incoherent anisotropy Ki, is analyzed, taking account of magnetostatic interactions. Solving the equations shows that considering a unique typical kind of CV does not enable accounting for both the domain pattern and the coercivity. Actually, the classical majority CVs obeying the random anisotropy model explains well the domain pattern but considering another kind of CVs, minority, mingled with classical ones, featuring a magnitude of Ki comparable to Ku, is necessary to account for coercivity. The model has been successfully compared with experimental data.

Origin of absorption peaks in reflection loss spectrum in Ku- frequency band of Co-Zr substituted strontium hexaferrites prepared using sucrose precursor

Energy Technology Data Exchange (ETDEWEB)

Narang, Sukhleen Bindra, E-mail: sukhleen2@yahoo.com [Department of Electronics Technology, Guru Nanak Dev University, Amritsar (India); Pubby, Kunal, E-mail: kunalpubby02@gmail.com [Department of Electronics Technology, Guru Nanak Dev University, Amritsar (India); Chawla, S.K., E-mail: sschawla118@gmail.com [Department of Chemistry, Centre for Advanced Studies-I, Guru Nanak Dev University, Amritsar (India); Kaur, Prabhjyot, E-mail: prabhjyot.2525@gmail.com [Department of Chemistry, Centre for Advanced Studies-I, Guru Nanak Dev University, Amritsar (India)

2017-03-15

This study presents the detailed explanation of the factors, contributing towards the absorption peaks in reflection loss spectrum of hexaferrites. Cobalt-Zirconium substituted strontium hexaferrites, synthesized using sucrose precursor sol-gel technique, were analyzed in 12.4–18 GHz frequency range. The concepts of impedance matching through quarter wavelength condition, complex thickness, dielectric phase angle and attenuation constant have been used to determine the location as well as intensity of absorption peaks. This study also demonstrates the potential application of three compositions of this series with doping content (x)==0.0, 0.6 and 0.8 as an effective microwave absorbers in Ku-frequency band. - Highlights: • EM analysis of Sr Hexaferrites in Ku-band. • Factors towards absorption peak intensity & location.

Analysis of Standards Efficiency in Digital Television Via Satellite at Ku and Ka Bands

Directory of Open Access Journals (Sweden)

Landeros-Ayala Salvador

2013-06-01

Full Text Available In this paper, an analysis on the main technical features of digital television standards for satellite transmission is carried out. Based on simulations and link budgets, the standard with the best operational performance is defined, based on simulations and link budget analysis, as well as a comparative efficiency analysis is conducted for the Ku and Ka bands for both transparent and regenerative transponders in terms of power, bandwidth, information rate and link margin, including clear sky, uplink rain, downlink rain and rain in both.

Investigation on the performance of an optically generated RF local oscillator signal in Ku-band DVB-S systems

NARCIS (Netherlands)

Khan, M.R.H.; Marpaung, D.A.I.; Burla, M.; Roeloffzen, C.G.H.; Bernhardi, Edward; de Ridder, R.M.

2011-01-01

We investigate a way to externally generate the local oscillator (LO) signal used for downconversion of the Ku-band (10.7 − 12.75 GHz) RF signal received from a phased array antenna (PAA). The signal is then translated to an intermediate frequency (950 − 2150 MHz) at the output of the mixer of

The pretreatment of corn stover with Gloeophyllum trabeum KU-41 for enzymatic hydrolysis

Directory of Open Access Journals (Sweden)

Gao Ziqing

2012-05-01

Full Text Available Abstract Background Pretreatment is an essential step in the enzymatic hydrolysis of biomass for bio-ethanol production. The dominant concern in this step is how to decrease the high cost of pretreatment while achieving a high sugar yield. Fungal pretreatment of biomass was previously reported to be effective, with the advantage of having a low energy requirement and requiring no application of additional chemicals. In this work, Gloeophyllum trabeum KU-41 was chosen for corn stover pretreatment through screening with 40 strains of wood-rot fungi. The objective of the current work is to find out which characteristics of corn stover pretreated with G. trabeum KU-41 determine the pretreatment method to be successful and worthwhile to apply. This will be done by determining the lignin content, structural carbohydrate, cellulose crystallinity, initial adsorption capacity of cellulase and specific surface area of pretreated corn stover. Results The content of xylan in pretreated corn stover was decreased by 43% in comparison to the untreated corn stover. The initial cellulase adsorption capacity and the specific surface area of corn stover pretreated with G. trabeum were increased by 7.0- and 2.5-fold, respectively. Also there was little increase in the cellulose crystallinity of pretreated corn stover. Conclusion G. trabeum has an efficient degradation system, and the results indicated that the conversion of cellulose to glucose increases as the accessibility of cellulose increases due to the partial removal of xylan and the structure breakage of the cell wall. This pretreatment method can be further explored as an alternative to the thermochemical pretreatment method.

Study of the Effect of Simulated Rain on the Offset Parabolic Antenna at Ku-Band with Different Elevation Angles

Directory of Open Access Journals (Sweden)

H. Mostafa

2007-01-01

Full Text Available Effect of rain on the receiver antenna is a major factor to degrade the system performance in a frequency above 10 GHz. This paper deals with the wet antenna attenuation at Ku-band with three different frequencies at different rain rates. During the Ku-band propagation experiment, it was discovered that rain water on the antenna caused a significant attenuation. It is necessary to estimate the losses caused by water on the antenna in order to separate these losses from the atmospheric propagation losses. The experiment was done at USM Engineering Campus to study the attenuation for these physical parameters. A Ku-band RF signal was generated by a signal generator and transmitted via horn antenna. The signal was received using a smooth offset antenna of 60 cm by 54 cm (Astro dish and measured using spectrum analyzer. In order to simulate a rain, pipes with bores of a same distance were implemented. Three cases were considered: in the first case one pipe was used to simulate low rain rate, the second case two pipes were used to simulate medium rain rate, and the third case three pipes were used to simulate heavy rain rate. In addition, the tap was used to control the flow of water in order to get more values of rain rate. The total attenuation of RF signals due to water layer on the feed and on the reflector feed was found to be 3.1 dB at worst case. On the other hand, the attenuation of RF signal due to the feed only was 2.83 dB, so the major attenuation occur was due to feed.

New uranium chalcoantimonates, RbU{sub 2}SbS{sub 8} and KU{sub 2}SbSe{sub 8}, with a polar noncentrosymmetric structure

Energy Technology Data Exchange (ETDEWEB)

Choi, K S; Kanatzidis, M G

1999-09-01

The new compounds, RbU{sub 2}SbS{sub 8} and KU{sub 2}SbSe{sub 8}, were prepared as golden-black, blocklike crystals by the polychalcogenide molten flux method. RbU{sub 2}SbS{sub 8} has a two-dimensional character with layers running perpendicular to the c-axis. The coordination geometry around the U{sup 4+} atoms is best described as a bicapped trigonal prism. The trigonal prisms share triangular faces with neighboring prisms, forming one-dimensional columns along the a-axis. The columns are then joined to construct sheets by sharing capping S atoms. Sb{sup 3+} ions are sitting at the center of a slightly distorted seesaw coordination environment (CN = 4). Rb{sup +} ions are stabilized in 8-coordinate bicapped trigonal prismatic sites. KU{sub 2}SbSe{sub 8} has essentially the same structure as RbU{sub 2}SbS{sub 8}. However, Sb{sup 3+} and K{sup +} ions appear disordered in every other layer resulting in a different unit cell. RbU{sub 2}SbS{sub 8} is a semiconductor with a band gap of 1.38 eV. The band gap of KU{sub 2}SbSe{sub 8} could not be determined precisely due to the presence of overlapping intense f-f transitions in the region (0.5--1.1 eV). The Raman spectra show the disulfide stretching vibration in RbU{sub 2}SbS{sub 8} at 479 cm{sup {minus}1} and the diselenide stretching vibration in KU{sub 2}SbSe{sub 8} at 252 cm{sup {minus}1}. Magnetic susceptibility measurements indicate the presence of U{sup 4+} centers in the compounds. The compounds do not melt below 1,000 C under vacuum.

STS-79 Ku-band antenna, ODS and Spacehab module at PCR

Science.gov (United States)

1996-01-01

The orbiter Ku-band antenna looms large in this view of the Space Shuttle Atlantis' payload bay. Visible just past the antenna system -- stowed on the starboard side of the payload bay wall -- is the Orbiter Docking System (ODS), and connected to the ODS via a tunnel is the Spacehab Double Module in the aft area of the payload bay. This photograph was taken from the starboard wing platform on the fifth level of the Payload Changeout Room (PCR) at Launch Pad 39A. Work is under way in the PCR to close Atlantis' payload bay doors for flight. Atlantis currently is being targeted for liftoff on Mission STS-79, the fourth docking of the U.S. Shuttle to the Russian Space Station Mir, around September 12.

Analysis of Fade Dynamic at Ku-Band in Malaysia

Directory of Open Access Journals (Sweden)

Siat Ling Jong

2014-01-01

Full Text Available This work investigates fade dynamics of satellite communication systems in equatorial heavy rain region based on a one year of Ku-band propagation measurement campaign carried out in Universiti Teknologi Malaysia (UTM, Johor, Malaysia. First order statistics of rain attenuation are deduced and the results are found to be in good agreement with those obtained from other beacon measurements gathered within the same area (Kuala Lumpur. Moreover, the fade duration and slope statistics of the satellite signal variations are also carefully derived and subsequently compared with the ITU-R recommendation model. Such information is useful for the system operator and radio communication engineer for the design of appropriate fade mitigation techniques as well as the quality of service that could be offered to the user (according to the time interval for a typical day. Further evaluation on the performances of several ITU-R models in the heavy rain region are needed based on the measurement database available of this climatic region.

55K isoform of CDK9 associates with Ku70 and is involved in DNA repair

International Nuclear Information System (INIS)

Liu, Hongbing; Herrmann, Christine H.; Chiang, Karen; Sung, Tzu-Ling; Moon, Sung-Hwan; Donehower, Lawrence A.; Rice, Andrew P.

2010-01-01

Positive elongation factor b (P-TEFb) is a cellular protein kinase that is required for RNA polymerase II (RNAP II) transcriptional elongation of protein coding genes. P-TEFb is a set of different molecular complexes, each containing CDK9 as the catalytic subunit. There are two isoforms of the CDK9 protein - the major 42 KDa CDK9 isoform and the minor 55KDa isoform that is translated from an in-frame mRNA that arises from an upstream transcriptional start site. We found that shRNA depletion of the 55K CDK9 protein in HeLa cells induces apoptosis and double-strand DNA breaks (DSBs). The levels of apoptosis and DSBs induced by the depletion were reduced by expression of a 55K CDK9 protein variant resistant to the shRNA, indicating that these phenotypes are the consequence of depletion of the 55K protein and not off-target effects. We also found that the 55K CDK9 protein, but not the 42K CDK9 protein, specifically associates with Ku70, a protein involved in DSB repair. Our findings suggest that the 55K CDK9 protein may function in repair of DNA through an association with Ku70.

Türkiye’de Çalışan Y Kuşağında İş Tatmini-Motivasyon İlişkisi

OpenAIRE

Saracel, Nüket; Taşseven, Özlem; Kaynak, Ece

2016-01-01

Bilgi çağı olarak nitelenen yirmi birinci yüzyılda, bilgi ve insan organizasyonların en önemli sermayesihaline gelmiştir. Üretim kaynakları içerisinde insanın öneminin artması şirketlerin yönetiminde insangücünün motivasyonu ve iş tatminine ilişkin konuların özel önem taşıyan konular olmasını beraberindegetirmiştir. Birbirlerine yakın zamanlarda doğan insanların benzer özellikler taşıması kuşak kavramınıortaya çıkarmıştır. Her bir kuşağın farklı özelliklerinin olması, organizasyonel sistemler...

Predictive images of postoperative levator resection outcome using image processing software

Directory of Open Access Journals (Sweden)

Mawatari Y

2016-09-01

Full Text Available Yuki Mawatari,1 Mikiko Fukushima2 1Igo Ophthalmic Clinic, Kagoshima, 2Department of Ophthalmology, Faculty of Life Science, Kumamoto University, Chuo-ku, Kumamoto, Japan Purpose: This study aims to evaluate the efficacy of processed images to predict postoperative appearance following levator resection.Methods: Analysis involved 109 eyes from 65 patients with blepharoptosis who underwent advancement of levator aponeurosis and Müller’s muscle complex (levator resection. Predictive images were prepared from preoperative photographs using the image processing software (Adobe Photoshop®. Images of selected eyes were digitally enlarged in an appropriate manner and shown to patients prior to surgery.Results: Approximately 1 month postoperatively, we surveyed our patients using questionnaires. Fifty-six patients (89.2% were satisfied with their postoperative appearances, and 55 patients (84.8% positively responded to the usefulness of processed images to predict postoperative appearance.Conclusion: Showing processed images that predict postoperative appearance to patients prior to blepharoptosis surgery can be useful for those patients concerned with their postoperative appearance. This approach may serve as a useful tool to simulate blepharoptosis surgery. Keywords: levator resection, blepharoptosis, image processing, Adobe Photoshop® 

Impact of tropospheric scintillation in the Ku/K bands on the communications between two LEO satellites in a radio occultation geometry

DEFF Research Database (Denmark)

Martini, Enrica; Freni, A.; Facheris, L.

2006-01-01

A theoretical analysis of the impact of clear-air tropospheric scintillation on a radio occultation link between two low Earth orbit satellites in K- and Ku-bands is presented, with particular reference to differential approaches for the measure of the total content of water vapor. The troposphere...

Prokaryotic Homologs of the Eukaryotic 3-Hydroxyanthranilate 3,4-Dioxygenase and 2-Amino-3-Carboxymuconate-6-Semialdehyde Decarboxylase in the 2-Nitrobenzoate Degradation Pathway of Pseudomonas fluorescens Strain KU-7†

OpenAIRE

Muraki, Takamichi; Taki, Masami; Hasegawa, Yoshie; Iwaki, Hiroaki; Lau, Peter C. K.

2003-01-01

The 2-nitrobenzoic acid degradation pathway of Pseudomonas fluorescens strain KU-7 proceeds via a novel 3-hydroxyanthranilate intermediate. In this study, we cloned and sequenced a 19-kb DNA locus of strain KU-7 that encompasses the 3-hydroxyanthranilate meta-cleavage pathway genes. The gene cluster, designated nbaEXHJIGFCDR, is organized tightly and in the same direction. The nbaC and nbaD gene products were found to be novel homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase a...

Chemical compatibility study of Cooley L18KU, Herculite, and Elephant Mat with Hanford tank waste

International Nuclear Information System (INIS)

Mercado, J.E.

1998-01-01

An independent chemical compatibility review of various wrapping and absorbent/padding materials was conducted to evaluate resistance to chemicals and constituents present in liquid waste from the Hanford underground tanks. These materials will be used to wrap long-length contaminated equipment when such equipment is removed from the tanks and prepared for transportation and subsequent disposal or storage. The materials studied were Cooley L18KU, Herculite, and Elephant Mat. The study concludes that these materials are appropriate for use in this application

Room Temperature Thin Film Ba(x)Sr(1-x)TiO3 Ku-Band Coupled MicrostripPhase Shifters: Effects of Film Thickness, Doping, Annealing and Substrate Choice

Science.gov (United States)

VanKeuls, F. W.; Mueller, C. H.; Miranda, F. A.; Romanofsky, R. R.; Canedy, C. L.; Aggarwal, S.; Venkatesan, T.; Ramesh, R.; Horwitz, S.; Chang, W.

1999-01-01

We report on measurements taken on over twenty Ku-band coupled microstrip phase shifters (CMPS) using thin ferroelectric films of Ba(x)Sr(1-x)TiO3. This CMPS design is a recent innovation designed to take advantage of the high tunability and tolerate the high dielectric constant of ferroelectric films at Ku- and K-band frequencies. These devices are envisioned as a component in low-cost steerable beam phased area antennas, Comparisons are made between devices with differing film thickness, annealed vs unannealed, Mn-doped vs. undoped, and also substrates of LaAlO3 and MgO. A comparison between the CMPS structure and a CPW phase shifter was also made oil the same ferroelectric film.

Focusing electrode and coaxial reflector used for reducing the guiding magnetic field of the Ku-band foilless transit-time oscillator

Energy Technology Data Exchange (ETDEWEB)

Ling, Junpu; Zhang, Jiande; He, Juntao, E-mail: hejuntao12@163.com; Wang, Lei; Deng, Bingfang [College of Optoelectronic Science and Engineering, National University of Defense Technology, Changsha 410073 (China)

2014-08-15

Based on the theoretical analysis of the intense relativistic electron beam propagation in the coaxial drift-tube, a focusing electrode and a coaxial reflector is proposed to lessen the demand of the coaxial Ku-band foilless transit-time oscillator (TTO) for the guiding magnetic field. Moreover, a Ku-band TTO with the focusing electrode and the coaxial reflector is designed and studied by particle in cell simulation. When the diode voltage is 390 kV, the beam current 7.8 kA, and the guiding magnetic field is only 0.3 T, the device can output 820 MW microwave pulse at 14.25 GHz by means of the simulation. However, for the device without them, the output power is only 320 MW. The primary experiments are also carried out. When the guiding magnetic field is 0.3 T, the output power of the device with the focusing electrode and the coaxial reflector is double that of the one without them. The simulation and experimental results prove that the focusing electrode and the coaxial reflector are effective on reducing the guiding magnetic field of the device.

Design studies of the Ku-band, wide-band Gyro-TWT amplifier

Science.gov (United States)

Jung, Sang Wook; Lee, Han Seul; Jang, Kwong Ho; Choi, Jin Joo; Hong, Yong Jun; Shin, Jin Woo; So, Jun Ho; Won, Jong Hyo

2014-02-01

This paper reports a Ku-band, wide band Gyrotron-Traveling-wave-tube(Gyro-TWT) that is currently being developed at Kwangwoon University. The Gyro-TWT has a two stage linear tapered interaction circuit to obtain a wide operating bandwidth. The linearly-tapered interaction circuit and nonlinearly-tapered magnetic field gives the Gyro-TWT a wide operating bandwidth. The Gyro-TWT bandwidth is 23%. The 2d-Particle-in-cell(PIC) and MAGIC2d code simulation results are 17.3 dB and 24.34 kW, respectively for the maximum saturated output power. A double anode MIG was simulated with E-Gun code. The results were 0.7 for the transvers to the axial beam velocity ratio (=alpha) and a 2.3% axial velocity spread at 50 kV and 4 A. A magnetic field profile simulation was performed by using the Poisson code to obtain the grazing magnetic field of the entire interaction circuit with Poisson code.

Impact of Surface Soil Moisture Variations on Radar Altimetry Echoes at Ku and Ka Bands in Semi-Arid Areas

Directory of Open Access Journals (Sweden)

Christophe Fatras

2018-04-01

Full Text Available Radar altimetry provides information on the topography of the Earth surface. It is commonly used for the monitoring not only sea surface height but also ice sheets topography and inland water levels. The radar altimetry backscattering coefficient, which depends on surface roughness and water content, can be related to surface properties such as surface soil moisture content. In this study, the influence of surface soil moisture on the radar altimetry echo and backscattering coefficient is analyzed over semi-arid areas. A semi-empirical model of the soil’s complex dielectric permittivity that takes into account that small-scale roughness and large-scale topography was developed to simulate the radar echoes. It was validated using waveforms acquired at Ku and Ka-bands by ENVISAT RA-2 and SARAL AltiKa respectively over several sites in Mali. Correlation coefficients ranging from 0.66 to 0.94 at Ku-band and from 0.27 to 0.96 at Ka-band were found. The increase in surface soil moisture from 0.02 to 0.4 (i.e., the typical range of variations in semi-arid areas increase the backscattering from 10 to 15 dB between the core of the dry and the maximum of the rainy seasons.

Zamyšlení nad charakterem vůdčí vrstvy městského obyvatelstva na příkladu Brna v raném novověku. Diskusní příspěvek

Czech Academy of Sciences Publication Activity Database

Sulitková, Ludmila

2004-01-01

Roč. 22, - (2004), s. 243-260, 383-384 ISSN 0231-7443. [Pražské městské elity středověku a raného novověku - jejich proměny, zázemí a kulturní profil. Praha, 01.10.2002-02.10.2002] Institutional research plan: CEZ:AV0Z8077915 Keywords : municipal elites * Early Modern Ages * Brno Subject RIV: AB - History

Low-loss negative index metamaterials for X, Ku, and K microwave bands

Directory of Open Access Journals (Sweden)

David A. Lee

2015-04-01

Full Text Available Low-loss, negative-index of refraction metamaterials were designed and tested for X, Ku, and K microwave frequency bands. An S-shaped, split-ring resonator was used as a unit cell to design homogeneous slabs of negative-index metamaterials. Then, the slabs of metamaterials were cut unto prisms to measure experimentally the negative index of refraction of a plane electromagnetic wave. Theoretical simulations using High-Frequency Structural Simulator, a finite element equation solver, were in good agreement with experimental measurements. The negative index of refraction was retrieved from the angle- and frequency-dependence of the transmitted intensity of the microwave beam through the metamaterial prism and compared well to simulations; in addition, near-field electromagnetic intensity mapping was conducted with an infrared camera, and there was also a good match with the simulations for expected frequency ranges for the negative index of refraction.

Sutureless intrascleral intraocular lens fixation with lamellar dissection of scleral tunnel

Directory of Open Access Journals (Sweden)

Kawaji T

2016-01-01

Full Text Available Takahiro Kawaji,1,2 Tomoki Sato,2 Hidenobu Tanihara11Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, Chuo-ku, 2Sato Eye & Internal Medicine Clinic, Kumamoto, JapanPurpose: To report the results of sutureless scleral fixation of a posterior chamber intraocular lens (IOL by using our developed simple technique.Methods: We retrospectively reviewed the medical records of 48 eyes of 47 patients who underwent sutureless intrascleral IOL fixation by using our modified technique. A 25-gauge microvitreoretinal knife was used to perform sclerotomies and create limbus-parallel scleral tunnels with lamellar dissection in which the haptics were fixed.Results: The IOLs were fixed and centered well. The mean follow-up period was 26.7 months. Postoperative complications included smooth vitreous hemorrhage in four eyes (8.3%, cystoid macular edema in two eyes (4.2%, and iris capture of the IOL in two eyes (4.2%. No other complications, such as breakage of the IOL, spontaneous IOL dislocation, or retinal detachment, were detected during the follow-up period.Conclusion: The lamellar dissection of the limbus-parallel scleral tunnel can simplify the forceps-assisted introduction of the haptics into the scleral tunnel, and this technique seemed to be safe.Keywords: intraocular lenses, ophthalmologic surgical procedures, intrascleral fixation, sutureless fixation

Generation, Transformation and Place in Inga Iwasiów’s Novels Bambino (2008 and Ku słońcu (2010

Directory of Open Access Journals (Sweden)

Ursula PHILLIPS

2012-06-01

Full Text Available This chapter will discuss two novels by contemporary writer Inga Iwasiów (b. 1963, Bambino (2008 and Ku słońcu [Towards the Sun] (2010, in the context of geopolitical, ideological, social and psycho-cultural transformations as they specifically affect different generations of inhabitants of the Polish city of Szczecin (pre-1945 German Stettin from 1945 until the first decade of the 21st century. Bambino covers the years 1945–1981, but also contains flashbacksto pre-war memory of Stettin and to the suppressed experiences of the new post-war inhabitants, as well as ‘flash-forwards’ to post-1989, post-communist reality; Ku słońcu covers 1981 to the first decade of 2000s. It will show how the novels portray these transformations through the experience primarily of women (but not only — in Bambino, through the main protagonists: Ula (formerly Ulrike, Anna and Marysia, and through that of the hidden narrator Magda (Marysia’s daughter born in the same year as the author, Iwasiów, 1963; and in Ku słońcu, primarily through the experience of Magda herself, as well as through that of two generations of ‘feminist’ academics: Małgorzata and Sylwia (in which it is hard not to perceive the personal experience of the author, though she is at pains to emphasize elsewhere that her work is a collective biography of the city, not so much of herself. The analysis follows Katarzyna Chmielewska’s supposition that contemporary Polish fiction dealing with the past ‘constantly oscillates between memory and genealogy’, and that it raises raw issues not yet addressed by historians; it also derives inspiration from the new ‘postdependency’ studiesproposed by Hanna Gosk and Ryszard Nycz. It also attempts to capture the specific nature of Iwasiów’s use of language, her style of feminism, her method of portraying place, and the connections in her prose between place and memory.

Akadēmiskās pašregulācijas saistība ar pusaudžu personības iezīmēm un vecāku audzināšanas stiliem

OpenAIRE

Iliško, Judīte

2016-01-01

Maģistra darba pētījuma mērķis bija noskaidrot, kāda saistība pastāv starp pusaudžu pašnovērtēto akadēmisko pašregulāciju, vecāku audzināšanas stiliem un pusaudžu personības iezīmēm, kā arī, vai pastāv dzimuma atšķirības starp pusaudžu pašnovērtēto akadēmisko pašregulāciju, vecāku audzināšanas stiliem un personības iezīmēm, un, cik lielā mērā pusaudžu personības iezīmes un vecāku audzināšanas stili prognozē pusaudžu akadēmisko pašregulāciju. Pētījumā piedalījās 225 7.-8.klašu skolēni no vien...

Waldenström's macroglobulinemia harbors a unique proteome where Ku70 is severely underexpressed as compared with other B-lymphoproliferative disorders

International Nuclear Information System (INIS)

Perrot, A; Pionneau, C; Azar, N; Baillou, C; Lemoine, F M; Leblond, V; Merle-Béral, H; Béné, M-C; Herbrecht, R; Bahram, S; Vallat, L

2012-01-01

Waldenström's macroglobulinemia (WM) is a clonal B-cell lymphoproliferative disorder (LPD) of post-germinal center nature. Despite the fact that the precise molecular pathway(s) leading to WM remain(s) to be elucidated, a hallmark of the disease is the absence of the immunoglobulin heavy chain class switch recombination. Using two-dimensional gel electrophoresis, we compared proteomic profiles of WM cells with that of other LPDs. We were able to demonstrate that WM constitutes a unique proteomic entity as compared with chronic lymphocytic leukemia and marginal zone lymphoma. Statistical comparisons of protein expression levels revealed that a few proteins are distinctly expressed in WM in comparison with other LPDs. In particular we observed a major downregulation of the double strand repair protein Ku70 (XRCC6); confirmed at both the protein and RNA levels in an independent cohort of patients. Hence, we define a distinctive proteomic profile for WM where the downregulation of Ku70—a component of the non homologous end-joining pathway—might be relevant in disease pathophysiology

Reduction of intergranular exchange coupling and grain size for high Ku CoPt-based granular media: Metal-oxide buffer layer and multiple oxide boundary materials

Science.gov (United States)

Tham, Kim Kong; Kushibiki, Ryosuke; Kamada, Tomonari; Hinata, Shintaro; Saito, Shin

2018-05-01

Investigation of magnetic properties and microstructure of granular media with various multiple oxides as the grain boundary material is reported. Saturation magnetization (Ms), uniaxial magnetocrystalline anisotropy (Ku), and magnetic grain diameter (GD) of the granular media show linear correlation with volume weighted average for melting point (Tm) of each oxides (Tmave). Ku of magnetic grains (Kugrain) shows a trade-off relation with GD that it is a big challenge to satisfy both high Kugrain and small GD by only controlling Tmave. To obtain a granular medium with appropriate Kugrain, GD, and low degree of intergranular exchange coupling, the combination of Tmave control of grain boundary material by mixing oxides and employment of a buffer layer are required. Here the degree of intergranular exchange coupling is estimated from the slope of M-H loop at around coercivity (α). By applying this technique, a typical granular medium with Kugrain of 1.0×107 erg/cm3, GD of 5.1 nm, and α of 1.2 is realized.

A high efficiency Ku-band radial line relativistic klystron amplifier

Energy Technology Data Exchange (ETDEWEB)

Dang, Fangchao; Zhang, Xiaoping, E-mail: zhangxiaoping@nudt.edu.cn; Zhong, Huihuang; Zhang, Jun; Ju, Jinchuan [College of Optoelectronic Science and Engineering, National University of Defense Technology, Changsha 410073 (China)

2016-07-15

To achieve the gigawatt-level microwave amplification output at Ku-band, a radial-line relativistic klystron amplifier is proposed and investigated in this paper. Different from the annular electron beam in conventional axial relativistic klystron amplifiers, a radial-radiated electron beam is employed in this proposed klystron. Owing to its radially spreading speciality, the electron density and space charge effect are markedly weakened during the propagation in the radial line drift tube. Additionally, the power capacity, especially in the output cavity, is enhanced significantly because of its large volume, which is profitable for the long pulse operation. Particle-in-cell simulation results demonstrate that a high power microwave with the power of 3 GW and the frequency of 14.25 GHz is generated with a 500 kV, 12 kA electron beam excitation and the 30 kW radio-frequency signal injection. The power conversion efficiency is 50%, and the gain is about 50 dB. Meanwhile, there is insignificant electron beam self-excitation in the proposed structure by the adoption of two transverse electromagnetic reflectors. The relative phase difference between the injected signals and output microwaves keeps stable after the amplifier saturates.

Effects of surface roughness on sea ice freeboard retrieval with an Airborne Ku-Band SAR radar altimeter

DEFF Research Database (Denmark)

Hendricks, Stefan; Stenseng, Lars; Helm, Veit

2010-01-01

to investigate sea ice volume changes on an Arctic wide scale. Freeboard retrieval requires precise radar range measurements to the ice surface, therefore we investigate the penetration of the Ku-Band radar waves into the overlying snow cover as well as the effects of sub-footprint-scale surface roughness using...... airborne radar and laser altimeters. We find regional variable penetration of the radar signal at late spring conditions, where the difference of the radar and the reference laser range measurement never agrees with the expected snow thickness. In addition, a rough surface can lead to biases...

Deficient Pms2, ERCC1, Ku86, CcOI in field defects during progression to colon cancer.

Science.gov (United States)

Nguyen, Huy; Loustaunau, Cristy; Facista, Alexander; Ramsey, Lois; Hassounah, Nadia; Taylor, Hilary; Krouse, Robert; Payne, Claire M; Tsikitis, V Liana; Goldschmid, Steve; Banerjee, Bhaskar; Perini, Rafael F; Bernstein, Carol

2010-07-28

In carcinogenesis, the "field defect" is recognized clinically because of the high propensity of survivors of certain cancers to develop other malignancies of the same tissue type, often in a nearby location. Such field defects have been indicated in colon cancer. The molecular abnormalities that are responsible for a field defect in the colon should be detectable at high frequency in the histologically normal tissue surrounding a colonic adenocarcinoma or surrounding an adenoma with advanced neoplasia (well on the way to a colon cancer), but at low frequency in the colonic mucosa from patients without colonic neoplasia. Using immunohistochemistry, entire crypts within 10 cm on each side of colonic adenocarcinomas or advanced colonic neoplasias were found to be frequently reduced or absent in expression for two DNA repair proteins, Pms2 and/or ERCC1. Pms2 is a dual role protein, active in DNA mismatch repair as well as needed in apoptosis of cells with excess DNA damage. ERCC1 is active in DNA nucleotide excision repair. The reduced or absent expression of both ERCC1 and Pms2 would create cells with both increased ability to survive (apoptosis resistance) and increased level of mutability. The reduced or absent expression of both ERCC1 and Pms2 is likely an early step in progression to colon cancer. DNA repair gene Ku86 (active in DNA non-homologous end joining) and Cytochrome c Oxidase Subunit I (involved in apoptosis) had each been reported to be decreased in expression in mucosal areas close to colon cancers. However, immunohistochemical evaluation of their levels of expression showed only low to modest frequencies of crypts to be deficient in their expression in a field defect surrounding colon cancer or surrounding advanced colonic neoplasia. We show, here, our method of evaluation of crypts for expression of ERCC1, Pms2, Ku86 and CcOI. We show that frequency of entire crypts deficient for Pms2 and ERCC1 is often as great as 70% to 95% in 20 cm long areas

Serial blood donations for intrauterine transfusions of severe hemolytic disease of the newborn with the use of recombinant erythropoietin in a pregnant woman alloimmunized with anti-Ku.

Science.gov (United States)

Lydaki, Evaggelia; Nikoloudi, Irene; Kaminopetros, Petros; Bolonaki, Irene; Sifakis, Stavros; Kikidi, Katerina; Koumantakis, Evgenios; Foundouli, Kaliopi

2005-11-01

The management of a pregnant woman with the rare Ko phenotype and anti-Ku is a special challenge, because matched blood is extremely rare and the possibility of severe hemolytic disease of the newborn is high. A 30-year-old woman with rare Ko (Knull) phenotype presented at 18 weeks of gestation with positive indirect agglutination test results. She had anti-Ku due to previous blood transfusion, one pregnancy, and two abortions. During this pregnancy, anti-Ku titers ranged from 1024 to 4096. At the 26th week of gestation ultrasound showed a hydropic fetus and urgent intrauterine exchange transfusion was performed with the maternal red blood cells (RBCs). Recombinant human erythropoietin (rHu-EPO) and intravenous (IV) iron were administered to the mother to ensure an adequate supply of matched RBCs for intrauterine transfusions and possible perinatal hemorrhage. Intrauterine transfusions were repeated every 1 to 3 weeks. By 35 weeks 2 days of gestation, the mother had donated 4 units of blood, and four intrauterine transfusions had been performed. Cesarean section was then decided and a healthy male newborn was born. He was treated with phototherapy but without exchange transfusions. By the 15th day of life rHu-EPO was administrated to the newborn because of anemia. The maternal RBCs completely disappeared from the child's blood by Day 100. As shown in this case, treatment with rHu-EPO and IV Fe has effectively increased the mother's capacity to donate RBCs for autologous use and intrauterine transfusions, with no adverse effects to the mother or the child.

Resistance to Sri Lankan Cassava Mosaic Virus (SLCMV) in Genetically Engineered Cassava cv. KU50 through RNA Silencing

KAUST Repository

Ntui, Valentine Otang

2015-04-22

Cassava ranks fifth among the starch producing crops of the world, its annual bioethanol yield is higher than for any other crop. Cassava cultivar KU50, the most widely grown cultivar for non-food purposes is susceptible to Sri Lankan cassava mosaic virus (SLCMV). The objective of this work was to engineer resistance to SLCMV by RNA interference (RNAi) in order to increase biomass yield, an important aspect for bioethanol production. Here, we produced transgenic KU50 lines expressing dsRNA homologous to the region between the AV2 and AV1 of DNA A of SLCMV. High level expression of dsRNA of SLCMV did not induce any growth abnormality in the transgenic plants. Transgenic lines displayed high levels of resistance to SLCMV compared to the wild-type plants and no virus load could be detected in uninoculated new leaves of the infected resistant lines after PCR amplification and RT-PCR analysis. The agronomic performance of the transgenic lines was unimpaired after inoculation with the virus as the plants presented similar growth when compared to the mock inoculated control plants and revealed no apparent reduction in the amount and weight of tubers produced. We show that the resistance is correlated with post-transcriptional gene silencing because of the production of transgene specific siRNA. The results demonstrate that transgenic lines exhibited high levels of resistance to SLCMV. This resistance coupled with the desirable yield components in the transgenic lines makes them better candidates for exploitation in the production of biomass as well as bioethanol.

Resistance to Sri Lankan Cassava Mosaic Virus (SLCMV) in Genetically Engineered Cassava cv. KU50 through RNA Silencing

KAUST Repository

Ntui, Valentine Otang; Kong, Kynet; Khan, Raham Sher; Igawa, Tomoko; Janavi, Gnanaguru Janaky; Rabindran, Ramalingam; Nakamura, Ikuo; Mii, Masahiro

2015-01-01

Cassava ranks fifth among the starch producing crops of the world, its annual bioethanol yield is higher than for any other crop. Cassava cultivar KU50, the most widely grown cultivar for non-food purposes is susceptible to Sri Lankan cassava mosaic virus (SLCMV). The objective of this work was to engineer resistance to SLCMV by RNA interference (RNAi) in order to increase biomass yield, an important aspect for bioethanol production. Here, we produced transgenic KU50 lines expressing dsRNA homologous to the region between the AV2 and AV1 of DNA A of SLCMV. High level expression of dsRNA of SLCMV did not induce any growth abnormality in the transgenic plants. Transgenic lines displayed high levels of resistance to SLCMV compared to the wild-type plants and no virus load could be detected in uninoculated new leaves of the infected resistant lines after PCR amplification and RT-PCR analysis. The agronomic performance of the transgenic lines was unimpaired after inoculation with the virus as the plants presented similar growth when compared to the mock inoculated control plants and revealed no apparent reduction in the amount and weight of tubers produced. We show that the resistance is correlated with post-transcriptional gene silencing because of the production of transgene specific siRNA. The results demonstrate that transgenic lines exhibited high levels of resistance to SLCMV. This resistance coupled with the desirable yield components in the transgenic lines makes them better candidates for exploitation in the production of biomass as well as bioethanol.

Destruction of gel sulfonated cation-exchangers of the KU-2 type under the influence of hydrogen peroxide

Energy Technology Data Exchange (ETDEWEB)

Roginskaya, B.S.; Zavadovskaya, A.S.; Znamenskii, Yu.P.; Paskhina, N.A.; Dobrova, E.I.

1988-10-20

The purpose of this work was to study the mechanism of interaction of Soviet sulfonated cation-exchangers of the KU-2 type with hydrogen peroxide. It is shown that under the influence of hydrogen peroxide sulfonated cation-exchangers begin, after a certain induction period, to lose capacity and to release destruction products into water; the length of the induction period increases with the degree of cross-linking. In a given time of contact between the resin and the solution the degree of destruction falls with increase of cross-linking. The principal product of destruction of sulfonated cation-exchangers is an aromatic sulfonic acid containing oxidized groups in the side chains.

Reduction of intergranular exchange coupling and grain size for high Ku CoPt-based granular media: Metal-oxide buffer layer and multiple oxide boundary materials

Directory of Open Access Journals (Sweden)

Kim Kong Tham

2018-05-01

Full Text Available Investigation of magnetic properties and microstructure of granular media with various multiple oxides as the grain boundary material is reported. Saturation magnetization (Ms, uniaxial magnetocrystalline anisotropy (Ku, and magnetic grain diameter (GD of the granular media show linear correlation with volume weighted average for melting point (Tm of each oxides (Tmave. Ku of magnetic grains (Kugrain shows a trade-off relation with GD that it is a big challenge to satisfy both high Kugrain and small GD by only controlling Tmave. To obtain a granular medium with appropriate Kugrain, GD, and low degree of intergranular exchange coupling, the combination of Tmave control of grain boundary material by mixing oxides and employment of a buffer layer are required. Here the degree of intergranular exchange coupling is estimated from the slope of M-H loop at around coercivity (α. By applying this technique, a typical granular medium with Kugrain of 1.0×107 erg/cm3, GD of 5.1 nm, and α of 1.2 is realized.

Teplovodní výměník pro krbovou vložku

OpenAIRE

Kněžínek, Petr

2012-01-01

Diplomová práce je zaměřena na problematiku zapojení krbových kamen a krbových vložek do teplovodních vytápěcích systémů. Práce obsahuje stručný popis krbů a otopných soustav. Konkrétně se zabývá návrhem teplovodního výměníku pro krbová kamna. Poslední část práce je věnována experimentálnímu ověření provozních parametrů a vyhodnocení získaných výsledků. The diploma thesis is focused on problems connecting of fireplace with water heating systems. The thesis comprises concise description of ...

A technical writing programme implemented in a first-year engineering course at KU Leuven

Science.gov (United States)

Heylen, Christel; Vander Sloten, Jos

2013-12-01

Technical communication and technical writing are important skills for the daily work-life of every engineer. In the first-year engineering programme at KU Leuven, a technical writing programme is implemented within the project-based course 'Problem Solving and Engineering Design'. This paper describes a case study for implementing a writing programme based on active learning methods and situated learning in large classes. The programme consists of subsequent cycles of instructions, learning by doing and reflection on received feedback. In addition, a peer-review assignment, together with an interactive lecture using clicking devices, is incorporated within the assignments of the second semester. A checklist of desired writing abilities makes it easier to grade the large number of papers. Furthermore, this ensures that all staff involved in the evaluation process uses the same criteria to grade and for providing feedback.

Hemşirelerin Bireysel ve Profesyonel Değerlerinde Kuşaklararası Farklılıklar / Personal and Professional Difference between the Generation of Nurses

OpenAIRE

IŞIK, Gülşen; EĞELİOĞLU CETİŞLİ, Nuray; TOKEM, Yasemin; YILMAZ, Dilan; İLHAN, Aylin

2018-01-01

Amaç: Buaraştırmanın amacı hemşirelerin bireysel ve profesyonel değerleri arasındakikuşaklararası farklılıkları belirlemektir. Gereçve Yöntem: Tanımlayıcı ve karşılaştırmalı tipteki bu çalışma, İzmir’dekibir üniversite hastanesinde çalışan, X kuşağındaki 85(doğum tarihi 1965-1979yılları arasında olanlar) ve Y kuşağındaki 79 (doğum tarihi 1980-1999 yıllarıarasında olanlar) olmak üzere toplam 164 hemşire ile yürütülmüştür. Veriler, araştırmacılar tarafından 30 dakikalık süreiçerisinde yüz yüze ...

8 – 11 gadus vecu bērnu pašcieņas saistība ar vecāku audzināšanas stilu un tehnoferenci

OpenAIRE

Ērmane, Linda

2017-01-01

Ir izvirzīts pētījuma mērķis teorētiski un praktiski izpētīt 8 – 11 gadus vecu bērnu pašcieņas saistību ar vecāku audzināšanas stilu un tehnoferenci. Pētījumā ir viena hipotēze par to, ka pastāv saistība starp vecāku audzināšanas stilu, bērnu pašcieņu un tehoferenci. Pētījuma izlasi veido 15 bērni (M = 9,6 gadi; SD = 1,12; 8 meitenes un 7 zēni) un 30 vecāki (M = 36,8 gadi; SD = 5,74; 15 vīrieši un 15 sievietes). Ir izmantoti 3 instrumenti: Audzināšanas pieeju aptauja, Tehnoloģijas ierīču ieja...

Wideband Array for C, X, and Ku-Band Applications with 5.3:1 Bandwidth

Science.gov (United States)

Novak, Markus H.; Volakis, John L.; Miranda, Felix A.

2015-01-01

Planar arrays that exploit strong intentional coupling between elements have allowed for very wide bandwidths in low-profile configurations. However, such designs also require complex impedance matching networks that must also be very compact. For many space applications, typically occurring at C-, X-, Ku-, and most recently at Ka-band, such designs require specialized and expensive fabrication techniques. To address this issue, a novel ultra-wideband array is presented, using a simplified feed network to reduce fabrication cost. The array operates from 3.5-18.5 GHz with VSWR less than 2.4 at broadside, and is of very low profile, having a total height of lambda/10 at the lowest frequency of operation. Validation is provided using a 64-element prototype array, fabricated using common Printed Circuit Board (PCB) technology. The low size, weight, and cost of this array make it attractive for space-borne applications.

Emocionālā intelekta attīstība 16 -18 gadus veciem pusaudžiem un tā saistība ar vecāku audzināšanas stilu.

OpenAIRE

Poļakova, Anželika

2011-01-01

Šī pētījuma mērķis ir noskaidrot, vai vecāku izvēlētais audzināšanas stils ir saistīts ar emocionālā intelekta attīstību. Pētījumā piedalījās 64 respondenti: 32 sievietes no 16 līdz 18 gadiem un 32 vīrieši no 16 līdz 18 gadiem. Respondenti pārstāv dažādas sociāli ekonomiskas grupas. Tika izmantotas šādas metodes: Bar-Ona emocionālā intelekta aptauja (Bar-On Emotional Quotient Inventory (EQ-i), Bar-On, 1999) un Buri Vecāku audzināšanas stila aptauju (Buri Parental Authority Questionnaire- PAQ...

Pusaudžu problemātiskas uzvedības saistības ar viņu piesaistes stilu un vecāku audzināšanas stilu ģimenē

OpenAIRE

Zamberga, Zane

2006-01-01

Maģistra darba pētījuma mērķis bija noskaidrot pusaudžu uzvedības traucējumu, piesaistes stilu un vecāku audzināšanas stilu savstarpējo saistību pusaudžu pašnovērtējumā, un, kāda ir savstarpējā ietekme (multiplās regresijas analīzes rezultātā) piesaistes stilam un vecāku audzināšanas stilam, iepretim uzvedības pašnovērtējumam. Pētījuma izlasi veido 60 pusaudži vcecumā no 14 – 16 gadiem. Tika aptaujāti Latvijas vispārizglītojošo skolu 7. un 8. klases skolēni: 30 meitenes un 30 zēni. Pētījumā...

Modelling the morphology of migrating bacterial colonies

Science.gov (United States)

Nishiyama, A.; Tokihiro, T.; Badoual, M.; Grammaticos, B.

2010-08-01

We present a model which aims at describing the morphology of colonies of Proteus mirabilis and Bacillus subtilis. Our model is based on a cellular automaton which is obtained by the adequate discretisation of a diffusion-like equation, describing the migration of the bacteria, to which we have added rules simulating the consolidation process. Our basic assumption, following the findings of the group of Chuo University, is that the migration and consolidation processes are controlled by the local density of the bacteria. We show that it is possible within our model to reproduce the morphological diagrams of both bacteria species. Moreover, we model some detailed experiments done by the Chuo University group, obtaining a fine agreement.

Wpływ wysiłku fizycznego na funkcję układu oddechowego w twardzinie układowej

OpenAIRE

Stanisław Sierakowski; Ewa Gińdzieńska-Sieśkiewicz; Maciej Kaczmarski; Zenon Siergiejko; Grzegorz Siergiejko; Piotr Siergiejko; Justyna Fryc

2010-01-01

Cel: Powikłania ze strony układu oddechowego stanowią główną przyczynę zgonów pacjentów z twardziną układową (TU). Tętniczenadciśnienie płucne i śródmiąższowa choroba płuc stanowią najistotniejszez nich. Pacjenci są jednak często zdiagnozowani dopierow późnym okresie choroby, dlatego też istotne jest opracowaniemetody diagnostycznej do wczesnego wykrywania tych powikłań.Celem pracy była ocena wpływu wysiłku fizycznego na zmiany parametrówoddechowych. Materiały i metody: Badania przeprowadzono...

The impact of the snow cover on sea-ice thickness products retrieved by Ku-band radar altimeters

Science.gov (United States)

Ricker, R.; Hendricks, S.; Helm, V.; Perovich, D. K.

2015-12-01

Snow on sea ice is a relevant polar climate parameter related to ocean-atmospheric interactions and surface albedo. It also remains an important factor for sea-ice thickness products retrieved from Ku-band satellite radar altimeters like Envisat or CryoSat-2, which is currently on its mission and the subject of many recent studies. Such satellites sense the height of the sea-ice surface above the sea level, which is called sea-ice freeboard. By assuming hydrostatic equilibrium and that the main scattering horizon is given by the snow-ice interface, the freeboard can be transformed into sea-ice thickness. Therefore, information about the snow load on hemispherical scale is crucial. Due to the lack of sufficient satellite products, only climatological values are used in current studies. Since such values do not represent the high variability of snow distribution in the Arctic, they can be a substantial contributor to the total sea-ice thickness uncertainty budget. Secondly, recent studies suggest that the snow layer cannot be considered as homogenous, but possibly rather featuring a complex stratigraphy due to wind compaction and/or ice lenses. Therefore, the Ku-band radar signal can be scattered at internal layers, causing a shift of the main scattering horizon towards the snow surface. This alters the freeboard and thickness retrieval as the assumption that the main scattering horizon is given by the snow-ice interface is no longer valid and introduces a bias. Here, we present estimates for the impact of snow depth uncertainties and snow properties on CryoSat-2 sea-ice thickness retrievals. We therefore compare CryoSat-2 freeboard measurements with field data from ice mass-balance buoys and aircraft campaigns from the CryoSat Validation Experiment. This unique validation dataset includes airborne laser scanner and radar altimeter measurements in spring coincident to CryoSat-2 overflights, and allows us to evaluate how the main scattering horizon is altered by the

Gözeli yöresindeki (Elazığ meşe (Quercus sp . ormanlarına asılan yapay yuvalardaki böcekçil kuşların kuluçka faaliyetleri

Directory of Open Access Journals (Sweden)

Özden YALÇIN

2015-12-01

Full Text Available Elazığ Orman Bölge Müdürlüğü sınırları içerisinde yer alan Elazığ ili Sivrice ilçesi Gözeli mevkiinde bulunan meşe ormanlarında, 2009-2013 yılları arasında gerçekleştirilen bu çalışma ile farklı bakılara asılan farklı renkteki küçük ve büyük yuva sandıklarında yuva yapan kuş türleri ve bu türlerin kuluçka faaliyetlerinin saptanması amaçlanmıştır. Kuluçkalarını başarı ile tamamlayan kuş türlerinin Otus scops haricindeki türlerin hepsi, hem küçük hem de büyük yuvaları kullanmıştır. Öncelikli yuva tercihleri şu şekilde olmuştur: Parus major küçük yuvaların %66,4’ünde, Cyanistes caeruleus büyük yuvaların %16,3’ünde, Sitta europaea büyük yuvaların %23,2’sinde, Petronia petronia küçük yuvaların %15,2’sinde, Passer domesticus büyük yuvaların %27,9’unda, Otus scops ise sadece büyük yuvaların %7’sinde yuvalanmıştır. Asılan yuvalarda başarılı kuluçka durumunun farklı kuş türlerine göre bakı tercihleri arasında farklılıklar bulunmaktadır. Büyük yuvalarda güney (%39,5, doğu (%34,9, kuzey (%16,3 ve batı (%9,3’dır. Küçük yuvalarda ise güney (%33,2, doğu (%27,2, batı (%20,8 ve kuzey (%18,8’dir.Farklı renklerde asılan kuş yuvalarında başarılı kuluçka yapan kuş türlerinin renk tercihleri değişmektedir. Büyük yuvalarda, Parus major yeşil yuvaları, Cyanistes caeruleus sarı yuvaları, Sitta europaea turuncu yuvaları, Petronia petronia sarı ve mavi yuvaları, Passer domesticus sarı, mavi ve boyanmamış yuvaları daha fazla tercih etmişlerdir. Kuşların yuva tercihinde yuva boyutu, renk ve bakının türler arası farklılık gösterdiği ortaya çıkmıştır. Bir türün daha yoğun bulunduğu alanda, o türe uygun yuva tercihinin gerçekleştirilerek asılması biyolojik mücadele kapsamında daha verimli sonuçlar elde edilmesini sağlayacaktır.

Exposure to power frequency magnetic fields suppresses X-ray-induced apoptosis transiently in Ku80-deficient xrs5 cells

International Nuclear Information System (INIS)

Tian, Furong; Nakahara, Takehisa; Yoshida, Masami; Honda, Naoko; Hirose, Hideki; Miyakoshi, Junji

2002-01-01

In an attempt to determine whether exposure to extremely low frequency (ELF) electromagnetic fields can affect cells, Ku80-deficient cells (xrs5) and Ku80-proficient cells (CHO-K1) were exposed to ELF electromagnetic fields. Cell survival, and the levels of the apoptosis-related genes p21, p53, phospho-p53 (Ser 15 ), caspase-3 and the anti-apoptosis gene bcl-2 were determined in xrs5 and CHO-K1 cells following exposure to ELF electromagnetic fields and X-rays. It was found that exposure of xrs5 and CHO-K1 cells to 60 Hz ELF electromagnetic fields had no effect on cell survival, cell cycle distribution and protein expression. Exposure of xrs5 cells to 60 Hz ELF electromagnetic fields for 5 h after irradiation significantly inhibited G 1 cell cycle arrest induced by X-rays (1 Gy) and resulted in elevated bcl-2 expression. A significant decrease in the induction of p53, phospho-p53, caspase-3 and p21 proteins was observed in xrs5 cells when irradiation by X-rays (8 Gy) was followed by exposure to 5 mT ELF magnetic fields. Exposure of xrs5 cells to the ELF electromagnetic fields for 10 h following irradiation significantly decreased X-ray-induced apoptosis from about 1.7% to 0.7%. However, this effect was not found in CHO-K1 cells within 24 h of irradiation by X-rays alone and by X-rays combined with ELF electromagnetic fields. Exposure of xrs5 cells to 60 Hz ELF electromagnetic fields following irradiation can affect cell cycle distribution and transiently suppress apoptosis by decreasing the levels of caspase-3, p21, p53 and phospho-p53 and by increasing bcl-2 expression

USP22 Induces Cisplatin Resistance in Lung Adenocarcinoma by Regulating γH2AX-Mediated DNA Damage Repair and Ku70/Bax-Mediated Apoptosis

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Aman Wang

2017-05-01

Full Text Available Resistance to platinum-based chemotherapy is one of the most important reasons for treatment failure in advanced non-small cell lung cancer, but the underlying mechanism is extremely complex and unclear. The present study aimed to investigate the correlation of ubiquitin-specific peptidase 22 (USP22 with acquired resistance to cisplatin in lung adenocarcinoma. In this study, we found that overexpression of USP22 could lead to cisplatin resistance in A549 cells. USP22 and its downstream proteins γH2AX and Sirt1 levels are upregulated in the cisplatin- resistant A549/CDDP cell line. USP22 enhances DNA damage repair and induce cisplatin resistance by promoting the phosphorylation of histone H2AX via deubiquitinating histone H2A. In addition, USP22 decreases the acetylation of Ku70 by stabilizing Sirt1, thus inhibiting Bax-mediated apoptosis and inducing cisplatin resistance. The cisplatin sensitivity in cisplatin-resistant A549/CDDP cells was restored by USP22 inhibition in vivo and vitro. In summary, our findings reveal the dual mechanism of USP22 involvement in cisplatin resistance that USP22 can regulate γH2AX-mediated DNA damage repair and Ku70/Bax-mediated apoptosis. USP22 is a potential target in cisplatin-resistant lung adenocarcinoma and should be considered in future therapeutic practice.

Ocena poziomu lęku i depresji u rodziców dzieci z zespołem nerczycowym – sposoby radzenia sobie z nimi i metody wsparcia

Directory of Open Access Journals (Sweden)

Monika Pawlak-Bratkowska

2013-06-01

Full Text Available Wstęp i cele pracy: Idiopatyczny zespół nerczycowy w wieku rozwojowym przebiega nawrotowo i trwale zmie‑ nia życie dzieci oraz ich rodziców. Celem badania była ocena poziomu lęku i depresji u rodziców dzieci z zespo‑ łem nerczycowym. Materiał i metodyka: Badanie zaplanowano jako przekrojową próbę kliniczną wśród rodzi‑ ców dzieci chorujących na zespół nerczycowy w odniesieniu do rodziców dzieci zdrowych. Wszyscy badani (46 rodziców wypełniali: ankietę dotyczącą przebiegu choroby u dziecka, kwestionariusz STAI (Inwentarz stanu i cechy lęku, kwestionariusz Skali depresji Becka, kwestionariusz Skali kontroli emocji (CECS. Grupę kontrolną stanowiło 20 rodziców dzieci zdrowych. Wyniki: Rodzice dzieci z zespołem nerczycowym charakteryzowali się istotnie wyższym poziomem depresji (Skala depresji Becka 8,7 vs 4,9, t = 2,18, p < 0,05. U ojców zaobserwo‑ wano wyższe subiektywne uczucie kontrolowania własnych reakcji w porównaniu z matkami – wyższy wynik CECS, suma u ojców (55,1 vs 47,2, t = 2,53, p < 0,05. U matek odnotowano wyższe wskaźniki zarówno w ska‑ lach mierzących poziom lęku jako cechy (STAI – cecha 45,7 vs 36,1, t = 3,19, p < 0,05 i stanu (STAI – stan 42,8 vs 33,4, t = 2,8, p < 0,05, jak i w kwestionariuszu badającym poziom depresji (10,62 vs 4,86, t = 2,66, p < 0,05. Rodzice, którzy wykazywali tendencję do obwiniania się o chorobę dziecka, podczas jej pierwszego epizodu uzyskali statystycznie istotny wyższy wynik w testach badających poziom depresji. Wnioski: Rodzice dzieci cierpiących na zespół nerczycowy charakteryzowali się istotnie wyższym poziomem depresji niż rodzice dzieci zdrowych. Ważne wydaje się objęcie szczególną opieką matek, mającą na celu obniżenie poziomu lęku i objawów depresji, oraz ojców skłonnych do tłumienia przeżywanych emocji.

Fiscal 2000 report on results of R and D of welfare techno-system in West Ward, Kobe City; 2000 nendo walfare techno system kenkyu kaihatsu (Kobe-shi Nishi-ku) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

R and D was conducted on devices for promoting self-support, mitigating nursing care, and improving safety and comfort for the elderly and the disabled, with the fiscal 2000 results reported. In the development of a wheelchair system (small arm sling mountable on a wheelchair) capable of assisting self-support for eating and vocational activity at home, a mounting device of an arm sling was experimentally manufactured for five different types of wheelchairs. In the research on the welfare device support system at a disaster or emergency, the system was modified based on the result of the system operation in the preceding year. Consequently, it became possible to make use of a power from a solar battery, a storage battery and an utility line, and also to reduce instantaneous interruption caused by switching of the self-sustaining operation for example. Further, a load control adaptor linked with an inverter was developed, enabling the system to use electricity for a long period even at the time of power failure. In addition, a conventional simple charge/discharge control was found to cause a marked deterioration in storage cell properties; hence, an accurate charge controller was added. (NEDO)

Report on achievements in fiscal 1999 on research and development of the Welfare Techno-system (Nishi Ward, Kobe); 1999 nendo Welfare Techno system kenkyu kaihatsu (Kobe-shi Nishi-ku) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

Research and development has been performed on devices to enhance autonomy, safety and comfort for elderly and handicapped people in houses and homes. This paper summarizes the achievements in fiscal 1999. In developing wheelchairs to allow autonomy in meal taking and working at homes, a small upper arm holding device that can be installed on a wheelchair was fabricated on the trial basis according to the experience obtained in making the first prototype in the previous fiscal year, and clinical evaluation was given. In the research and development on a safe walk assisting appliance for elderly and handicapped people, development, improvement and trial fabrication were carried out on a caster in which the brake will not work during walking, but it works automatically in a sudden movement such as falling down. In developing an environment information transmitting system for elderly and handicapped people, a first prototype fabrication was performed on a system to detect sound of an alarm or vibration caused by knocking a door, and convey information to elderly and handicapped people by utilizing the communication services including the commercially available cellular phones. In the research on a welfare device support system for use in disaster and emergency, design and structuring were carried out on a nighttime electric power utilizing system having solar cells combined with storage batteries. Related data were measured. (NEDO)

Sadhana | Indian Academy of Sciences

Indian Academy of Sciences (India)

Keywords. Sensitivity analysis; dynamic improvement structural modoficaton; laser beam printer; motorbike; disc drive; mechatronics; automobile engine ... Author Affiliations. Nobuyuki Okubo1 Takeshi Toi1. Department of Precision Mechanics, Chuo University, Tokyo, Japan ...

Some clastic rocks as parent rocks in beech forests of Brezovica - Južni Kučaj

Directory of Open Access Journals (Sweden)

Joksimović Vesna

2003-01-01

Full Text Available On the large forest areas, where the measures of soil quality improvement are absent or rarely undertaken, the rocks are the sources of a great number of elements in plant nutrition and one of the factors affecting the contents of some elements in plants. The aim of the study is to determine the differences in the contents of Cu, Zn, Mn, Ni, Fe, Cd, Co, Pb and Cr in the beech leaves developed in the same climate conditions, on morphologically similar terrains, composed of different types of sedimentary rocks. This paper presents the study results of the contents of the above elements in argillites, sandstones (grauwackes and microconglomerates occurring as the parent rocks in high beech forests in the region of GJ Bogovina (Brezovica Južni Kučaj and in the leaves of beech developed on these rocks.

Motorické projevy laterality u osob středního věku a starších

OpenAIRE

Habartová, Lucie

2008-01-01

Název dielomové eráce: Motorické projevy laterality u osob středního věku a starších. Název v gnglickém jazyce: The Manifestations of Motoric Laterality of Middle-aged People and Elderly. Cíle eráce: Definovat, popsat lateralitu, zjistit stupeň laterality horní končetiny, dolní končetiny a oka. Zjistit vztah laterality horních končetin a očí, horních končetin a dolních končetin. Metoda: Lateralitu budeme zjišťovat na základě dotazníků a motorických testů. Testovaný soubor čítá 50 probandů ve ...

Y Kuşağı Turistlerin Destinasyon İmaj Algıları Üzerine Bir Araştırma A Research On Generation Y Tourists Perceptions Of Destination Image

Directory of Open Access Journals (Sweden)

Aslı ALBAYRAK

2013-07-01

Full Text Available This study has been performed on the purposed of determiningeffects of the destination image composing factors on deciding tovacation resort of Y Generation tourists who show differentcharacterists in comparison to the previous and the next generation,and making recommendations related to destination marketing for thegeneration by examining the relationship between destinationperception and structural features of Y Generation tourist.In this study, questionnaire technique which is one thequantitative research method, were used and questionnaire study weremade face to face with 384 people who were determined by quotasampling method and represent the generation in Istanbul. On analysisobtained from data were used descriptive statistics, t-test, and Anovaanalysis.According to results of research, destination image differs fromdepending on gender, level of educaiton, also income levels ofparticipants. On the other other hand, it can be said that Y Generationtourists act at the request of seeing new places, leisure, andentertainment and are the individuals who look for quality and luxuryholiday with affordable price.Touristic purchase process is shaped by many factors. One of thefactors affecting this process is image of destination. The mostgenerational definition about destination image is as an individual’soverall perception and these perceptions ınfluenced by many factorssuch as age, education, income and culture. There are several studieshave been conducted about tourists’ perceptions of destination image inpast and generally these studies about effects of tourists’ characteristicssuch as age, education, culture income to perception of destinationimage but there are no studies about different generations’ destinationimage perceptions. Therefore ıt’s thought that the results obtained fromthis study could contribute to the tourism marketers. Bu çalışma, kendinden önceki ve sonraki kuşaklardan farklı özellikler gösteren Y ku

A Small Ku-Band Polarization Tracking Active Phased Array for Mobile Satellite Communications

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Wei Shi

2013-01-01

Full Text Available A compact polarization tracking active phased array for Ku-band mobile satellite signal reception is presented. In contrast with conventional mechanically tracking antennas, the approach presented here meets the requirements of beam tracking and polarization tracking simultaneously without any servo components. The two-layer stacked square patch fed by two probes is used as antenna element. The impedance bandwidth of 16% for the element covers the operating frequency range from 12.25 GHz to 12.75 GHz. In the presence of mutual coupling, the dimensional parameters for each element of the small 7 × 7 array are optimized during beam scanning and polarization tracking. The compact polarization tracking modules based on the low-temperature cofired ceramic (LTCC system-in-package (SiP technology are proposed. A small active phased array prototype with the size of 120 mm (length × 120 mm (width × 55 mm (height is developed. The measured polarization tracking patterns of the prototype are given. The polarization tracking beam can be steered in the elevation up to 50°. The gain of no less than 16.0 dBi and the aperture efficiency of more than 50% are obtained. The measured and simulated polarization tracking patterns agreed well.

Efficient gene replacements in ku70 disruption strain of Aspergillus chevalieri var. intermedius

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Qingqing Huang

2017-01-01

Full Text Available Aspergillus chevalieri var. intermedius is a dominant filamentous fungal species in Fuzhuan tea and is associated with the quality and health benefits of this tea. The sexual or asexual reproduction of this fungus depends on the osmotic pressure of the tea. Efforts to enhance the beneficial effects of A. chevalieri var. intermedius are hampered by difficulties in disrupting its genes. To address this issue, we identified the A. chevalieri var. intermedius homolog (Acku70 of human Ku70 and generated an Acku70 disruption strain (ΔAcku70, aiming to improve the gene replacement efficiency. ΔAcku70 grew at a slightly lower rate in vitro than the wild-type strain; however, the two strains exhibited similar sensitivity to temperature, osmotic pressure and the effects of ethyl methane sulphonate and H2O2. The replacement efficiency of veA and flbA dramatically increased in ΔAcku70 compared to that in the wild type. The efficiency of flbA replacement increased from 2.6% to 80%, whereas the frequency of veA disruption increased from 15.2% to 83.9% and from 30.8% to 86.8%. Thus, ΔAcku70 is suitable for use as a type strain for large-scale functional genomic analysis of A. chevalieri var. intermedius.

Pusaudžu agresivitātes saistība ar vecāku audzināšanas stilu.

OpenAIRE

Dambe, Agnese

2007-01-01

Šajā darbā tiek apskatīta viena no mūsdienu būtiskākajām problēmām- pusaudžu agresivitāte. Pētījuma mērķis bija noskaidrot, vai pastāv statistiski nozīmīgas saistības starp vecāku audzināšanas stilu un pusaudžu agresivitāti. Pētījuma izlasi veido 60 pusaudži (30 jaunietes un 30 jaunieši) no pilnām ģimenēm, vecumposmā no 15-17 gadiem. Pētījumā tika izmantotas divas pētījuma metodes- A.H. Bassa (A.H. Buss)- A. Darki (A. Durkee) aptaujas anketa agresīvu reakciju noteikšanai un ...

Oxygen Isotope Records in Modern Oyster Shells from Chi Ku, Tainan and Their Implication of Seasonality

Science.gov (United States)

Chen, Y. C.; Mii, H. S.; Li, K. T.

2015-12-01

To exam whether oxygen isotope records of Crassostrea gigasoysters can be used as proxies of environment, 133 cultivated oysters and 21 water samples were collected from Chi Ku area, Tainan City, southern Taiwan in December of 2012, and from March, 2013 to July, 2014. Instrumental air and water temperatures and precipitation records were obtained from a nearest Central Weather Bureau (CWB) station roughly 16 km north of Chi Ku. The oxygen and carbon isotope values of the ligamental area of the modern oyster shells are from -6.92‰ to -0.08‰ (-3.05 ± 1.17‰, N = 2280; 1σ; VPDB) and from -5.57‰ to 0.63‰ (-1.88 ± 0.81‰), respectively. Oxygen isotope values of the water samples are mainly between -0.28‰ and 0.74‰ (0.18 ± 0.29‰, N = 20; 1σ; VSMOW). However, water oxygen isotope value of -2.75‰ was observed for the water sample collected immediately after a typhoon heavy rainfall. Seasonal temperature fluctuation pattern of estimated oxygen isotope temperatures from modern shells is similar to that of CWB instrumental records. However, the oxygen isotope temperatures are respectively about 3 °C and 10°C higher than those of instrumental records for winter and summer. Higher estimated oxygen isotope temperatures are most likely caused by underestimated fraction of freshwater. We analyzed 5 archaeological oyster shells of Siraya culture (500~250B.P.) collected from Wu Chien Tuso North (WCTN) archaeological site of Tainan branch of Southern Taiwan Science Park to infer the harvest season of mollusks. Oxygen isotope values of the ligamental area of the archaeological oyster shells are between -5.98‰ and -1.26‰ (-3.34 ± 1.37‰, N = 60; 1σ), and carbon isotope values are between -3.21‰ and 0.60‰ (-2.04‰ ± 0.55‰). The oxygen isotope records of archaeological oyster shells also showed clear seasonality. Most of the oysters were collected in autumn and winter. Oxygen isotope values of archaeological oyster shells was 1‰ greater than that

Tracking- and Scintillation-Aware Channel Model for GEO Satellite to Land Mobile Terminals at Ku-Band

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Ali M. Al-Saegh

2015-01-01

Full Text Available Recent advances in satellite to land mobile terminal services and technologies, which utilize high frequencies with directional antennas, have made the design of an appropriate model for land mobile satellite (LMS channels a necessity. This paper presents LMS channel model at Ku-band with features that enhance accuracy, comprehensiveness, and reliability. The effect of satellite tracking loss at different mobile terminal speeds is considered for directional mobile antenna systems, a reliable tropospheric scintillation model for an LMS scenario at tropical and temperate regions is presented, and finally a new quality indicator module for different modulation and coding schemes is included. The proposed extended LMS channel (ELMSC model is designed based on actual experimental measurements and can be applied to narrow- and wide-band signals at different regions and at different speeds and multichannel states. The proposed model exhibits lower root mean square error (RMSE and significant performance observation compared with the conventional model in terms of the signal fluctuations, fade depth, signal-to-noise ratio (SNR, and quality indicators accompanied for several transmission schemes.

Cave bear (Ursus spelaeus Rosenmüller & Heinroth males' den from Velika Pećina in Duboka Near Kučevo, Eastern Serbia

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Dimitrijević Vesna M.

2002-01-01

Full Text Available More a 100 years after the first research in the cave Velika pećina in Duboka near Kučevo cave bear remains were discovered in a small chamber cut off from the passable channels by a 7 m high slope. A whole skull, bones of a forearm in articulation, and other skeleton parts were laying on the cave floor encrusted in travertine cover and in some places overgrown by stalagmites. Bones belonged to adult males, which found there the shelter to hibernate, in a short epizode that ended by closing the channels that once linked this part of the cave to a surface.

Analysis and comparison model for measuring tropospheric scintillation intensity for Ku-band frequency in Malaysia

Directory of Open Access Journals (Sweden)

Mandeep JS

2011-06-01

Full Text Available This study has been based on understanding local propagation signal data distribution characteristics and identifying and predicting the overall impact of significant attenuating factors regarding the propagation path such as impaired propagation for a signal being transmitted. Predicting propagation impairment is important for accurate link budgeting, thereby leading to better communication network system designation. This study has thus used sample data for one year concerning beacon satellite operation in Malaysia from April 2008 to April 2009. Data concerning 12GHz frequency (Ku-band and 40° elevation angle was collected and analysed, obtaining average signal amplitude value, ÷ and also standard deviation ó which is normally measured in dB to obtain long-term scintillation intensity distribution. This analysis showed that scintillation intensity distribution followed Gaussian distribution for long-term data distribution. A prediction model was then selected based on the above; Karasawa,
ITU-R, Van de Kamp and Otung models were compared to obtain the best prediction model performance for selected data regarding specific meteorological conditions. This study showed that the Karasawa model had the best performance for predicting scintillation intensity for the selected da ta.

Y Kuşağının Mobil Öğrenme Uygulama Tercihini Etkileyen Faktörlerin İncelenmesi

OpenAIRE

ALTUNTAŞ, Başar

2017-01-01

Mobilteknolojilerin öğrenme ortamlarına dâhil edilmesiyle birlikte öğrenme faaliyetifiziksel sınırların dışına çıkmış ve farklı bir boyuta evrilmiştir. Tasarlanan öğrenmeuygulamaları kullanıcılarına zamandan ve mekândan bağımsız öğrenme fırsatısunarken, yeni ve farklı deneyimleri de beraberinde getirmiştir. Bu çalışmada,Y kuşağının mobil öğrenme uygulamalarını tercih etmesini ve kullanmasınıetkileyen faktörler incelenmiştir. Teknoloji kabul modeli ile beklenti-kabul modelitemelinde tasarlanan...

Kuş Gölü (Bandırma Makrobentik Omurgasız Faunası Hakkında Bir Ön Araştırma.

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Süleyman Balık

2015-12-01

Full Text Available Kuş Gölü’nün makrobentik omurgasız faunasını belirlemek amacıyla 1987 ve 1988 yılları arasında aylık periyotlar halinde, bir yıl süre ile 5 istasyonda arazi çalışması yapılmıştır. Ekman grab ile alınan çamur örnekleri 500 μm göz açıklığındaki elekten geçirilmiş ve örnekler %4’lük formaldehit solüsyonunda tespit edilmiştir. Araştırma sonucunda, Oligochaeta sınıfından 10, Chironomidae familyasından 5 ve Ceratopogonidae familyasından 1 olmak üzere toplam 16 takson tespit edilmiştir. Kuş gölü’nde metrekarede ortalama 6759 birey tespit edilmiş, grupların oransal dağılımında %81,94 ile Oligochaeta bireylerinin en baskın grubu oluşturduğu, bu grubu Chironomidae (%17,90 ve Ceratopogonidae (%0,16 larvalarının takip ettiği saptanmıştır. Tespit edilen grupların istasyonlara göre oransal dağılımlarında ise, 1. istasyonun en zengin (%28,71, 4. istasyonun en fakir (%12,77 istasyon olduğu gözlenmiştir

Pusaudžu - hokeja spēlētāju sasniegumu stratēģijas un apmierinātības izjūta, spēlējot hokeju, saistībā ar vecāku audzināšanas stiliem.

OpenAIRE

Stālmane, Liene

2014-01-01

Maģistra darba mērķis bija izpētīt saistības starp pusaudžu, kuri trenējas hokejā jau no agrīna vecuma, sasniegumu stratēģijām, apmierinātības izjūtu, spēlējot hokeju, un viņu vecāku audzināšanas stilus. Pētījumā tika iekļauti 53 respondenti latviešu tautības zēni, vecumā no 9 – 14 gadiem, kuri trenējas hokejā. Par pētījuma instrumentāriju izmantotas: 1)Vecāku audzināšanas stila aptauja. (Parental Authority Questionnaire (PAQ), Buri, 1991); 2) Sasniegumu stratēģiju aptauja....

Vecāku audzināšanas stilu saistība ar pašcieņu pusaudžu vecumā

OpenAIRE

Miķelsone-Ježaka, Dace

2008-01-01

Darba mērķis ir izpētīt pašcieņas saistību ar mātes un tēvu audzināšanas stilu bērnībā. Pētījumā piedalījās 105 pusaudži no dažādām skolām vecumā no 16 līdz 18 gadiem, kas dzīvo pilnās ģimenēs. Tika izmantotas 2 aptaujas: 1. Vecāku autoritātes novērtējuma aptauja (The Parental Authority Questionnaire, Buri, 1991; Griškina, 2002). 2. Rozenberga pašcieņas aptauja (The Rosenberg Self – esteem Scale, Rosenberg, 1965).

Vecāku audzināšanas stila saistība ar pusaudžu vientulības izjūtu

OpenAIRE

Vlasova, Iveta

2013-01-01

Pētījuma jautājums: Vai starp mātes audzināšanas stilu un vientulības līmeni pusaudžiem pastāv saistība? Pētījuma dalībnieki. Viena izlase, pēc pieejamības principa. Dalībnieku vecums: 12-16 gadi, 31 meitene, 23 zēni. Dzīvojoši pilnās ģimenēs. Pētījuma metode. 1.Lieto, lai noteiktu kopējo vientulības izjūtas līmeni, tika izmantota Vientulības skala (UCLA Loneliness Scale/UCLA LS/, VERSION 3; Russel, D.W. 1996).– visu jautājumu summa. 2.Vecāku audzināšanas stila aptauja (Parental...

Comparison of neutron and gamma irradiation effects on KU1 fused silica monitored by electron paramagnetic resonance

Energy Technology Data Exchange (ETDEWEB)

Bravo, D. [Department Fisica de Materiales, Universidad Autonoma de Madrid, E-28049 Madrid (Spain)], E-mail: david.bravo@uam.es; Lagomacini, J.C. [Department Fisica de Materiales, Universidad Autonoma de Madrid, E-28049 Madrid (Spain); Leon, M.; Martin, P. [Materiales para Fusion, CIEMAT, Avda. Complutense 22, E-28040 Madrid (Spain); Martin, A. [Department Fisica e Instalaciones, ETS Arquitectura UPM, E-28040 Madrid (Spain); Lopez, F.J. [Department Fisica de Materiales, Universidad Autonoma de Madrid, E-28049 Madrid (Spain); Ibarra, A. [Materiales para Fusion, CIEMAT, Avda. Complutense 22, E-28040 Madrid (Spain)

2009-06-15

Electron paramagnetic resonance (EPR) studies have been carried out on KU1 fused silica irradiated with neutrons at fluences 10{sup 21} and 10{sup 22} n/m{sup 2}, and gamma-ray doses up to 12 MGy. The effects of post-irradiation thermal annealing treatments, up to 850 deg. C, have also been investigated. Paramagnetic oxygen-related defects (POR and NBOHC) and E'-type defects have been identified and their concentration has been measured as a function of neutron fluence, gamma dose and post-irradiation annealing temperature. It is found that neutrons at the highest fluence generate a much higher concentration of defects (mainly E' and POR, both at concentrations about 5 x 10{sup 18} spins/cm{sup 3}) than gamma irradiations at the highest dose (mainly E' at a concentration about 4 x 10{sup 17} spins/cm{sup 3}). Moreover, for gamma-irradiated samples a lower treatment temperature (about 400 deg. C) is required to annihilate most of the observed defects than for neutron-irradiated ones (about 600 deg. C)

Ufundishaji wa Kusoma na Kuandika Kiswahili: Uzoefu wa Idara ya ...

African Journals Online (AJOL)

Makala haya yanahusu shughuli za kufundisha Kiswahili kama lugha ya kigeni katika Chuo Kikuu cha Sebha nchini Libya. Kwanza inaeleza historia ya Kiswahili – yaani kilianza lini kufundishwa kwa madhumuni gani na nani walikuwa wanzilishi. Halikadhalika makala ya naeleza utaratibu uliowekwa na idara ili kuweza ...

Faktorová analýza antropometrických charakteristik u mladých plavců ve věku 11 a 12 let Factor analysis of anthropometric characteristics in young swimmers aged 11 and 12

Directory of Open Access Journals (Sweden)

Zofia Ignasiak

2006-02-01

Full Text Available Cílem této práce bylo popsat vybrané somatické charakteristiky typické pro mladé plavce. Faktorová analýza umožnila úměrné snížení somatických ukazatelů a umožnila vypočítat hlavní strukturální faktory typické pro mladé plavce. Do výzkumného souboru bylo zařazeno 80 žáků (11 a 12letých přijatých ke studiu na základní sportovní škole ve Wroclawi. Sledovaní probandi trénovali po dobu 2–3 let a jejich průměrný tréninkový čas byl 12 až 18 hodin týdně. Byly měřeny následující parametry: tělesná výška a hmotnost, délka horních a dolních končetin, obvod stehen, lýtka, paží, předloktí, hrudníku v klidu, hrudníku po nádechu a po výdechu, obvod pasu a boků, šířka ramen, šířka boků, šířka hrudníku, hloubka hrudníku a Rohrerův index. K dosažení cíle této studie byla použita metoda faktorové analýzy. Analýza výsledků ukázala, že pouze některé zvláštnosti mezi sledovanými parametry jsou pro mladé plavce charakteristické. Jsou spojeny do 2 trsů somatických charakteristik: kubický obsah (zahrnuje tělesnou hmotnost, svalstvo, trup a obvod horních a dolních končetin a vertikální rozměr (zahrnuje tělesnou výšku a délku horních a dolních končetin. Tyto parametry signalizují formování postavy typické pro plavce, charakteristické specifickými proporcemi tělesné hmotnosti a výšky, hrudníku a boků a obvykle delšími končetinami. Výsledky naznačují, že antropometrické charakteristiky lze použít pro optimalizaci tréninkového procesu mladých plavců. The aim of this study was to describe the set of somatic characteristics, which significantly discriminate young swimmers. The factor analysis let us reduce the number of somatic traits mostly correlated and to calculate the main structural factors typical for young swimmers. There were 80 pupils (11 and 12 years old from the primary sport school in Wroclaw recruited for the study

A Novel Ku-Band/Ka-Band and Ka-Band/E-Band Multimode Waveguide Couplers for Power Measurement of Traveling-Wave Tube Amplifier Harmonic Frequencies

Science.gov (United States)

Wintucky, Edwin G.; Simons, Rainee N.

2015-01-01

This paper presents the design, fabrication and test results for a novel waveguide multimode directional coupler (MDC). The coupler, fabricated from two dissimilar frequency band waveguides, is capable of isolating power at the second harmonic frequency from the fundamental power at the output port of a traveling-wave tube (TWT) amplifier. Test results from proof-of-concept demonstrations are presented for a Ku-band/Ka-band MDC and a Ka-band/E-band MDC. In addition to power measurements at harmonic frequencies, a potential application of the MDC is in the design of a satellite borne beacon source for atmospheric propagation studies at millimeter-wave (mm-wave) frequencies (Ka-band and E-band).

Beretning for undersøgelse af bopladsområde med værkstedsaktivitet fra yngre jernalder/vikingetid, neolitiske anlæg fortrinsvis fra MN A III og en grav fra MN B II. Kalmergården (KU) 2000

DEFF Research Database (Denmark)

Bican, Josefine Franck

2001-01-01

anlæggelse er omtalt nedenfor i en separat beretning ved Lone Gebauer Thomsen (se bilag 2). Yngre jernalder/vikingetid er den periode, der var bedst repræsenteret på pladsen. Her var spor efter en markeds-/produktionsplads, der er registreret vha. 3735 detektorfund, hvor især sølvskatten (KU 2337) skal...

Styl radzenia sobie ze stresem jako moderator związku lęku z neurotyzmem u osób z reumatoidalnym zapaleniem stawów

Directory of Open Access Journals (Sweden)

Michał Ziarko

2011-06-01

Full Text Available Lęk stanowi jedną z konsekwencji chorób przewlekłych, w tymreumatoidalnego zapalenia stawów (RZS. Wysoki poziom lękuobniża jakość życia pacjentów, prowadzi do zachowań antyzdrowotnychoraz może wpływać na gorszy przebieg choroby. Związekneurotyzmu z lękiem został dotąd dobrze udokumentowany. Neurotyzmstanowi cechę temperamentu, a więc w niewielkim stopniumożna ją modyfikować. Redukcja kosztów psychicznych ponoszonychprzez osoby o wysokim poziomie neurotyzmu wymagapoznania czynników, które mogłyby ograniczać wpływ neurotyzmuna poziom lęku (czynniki moderujące relację neurotyzm–lęk.Celem badania było określenie zmiennych moderujących relacjępomiędzy neurotyzmem a lękiem.W badaniach wzięło udział 100 osób chorujących na RZS (78 ko -biet i 22 mężczyzn. Zostali oni przebadani za pomocą kwestionariuszymierzących: poziom lęku, style radzenia sobie ze stresemoraz wymiary osobowości. Obliczono współczynniki korelacjir Pearsona pomiędzy mierzonymi zmiennymi oraz wykonano analizęmoderacji związku pomiędzy neurotyzmem a lękiem jakostanem za pośrednictwem stylów radzenia sobie ze stresem.Testowanie interakcji przeprowadzono metodą najmniejszych kwadratówi regresji logistycznej.Ustalono, że istnieje wprost proporcjonalny związek pomiędzyneurotyzmem a lękiem rozumianym jako stan (r = 0,63; p < 0,01(tab. I. Zaobserwowano, że związek ten nie zachodzi w przypadkuosób o wysokim neurotyzmie posługujących się unikowym stylem radzenia sobie ze stresem (R2 = 0,19; F = 7,00; p < 0,001 (ryc. 1oraz u osób poszukujących kontaktów towarzyskich (R2 = 0,22;F = 8,70; p < 0,001 (ryc. 2, tab. II.Wyniki wskazują, że silny neurotyzm zwiększa prawdopodobieństworeagowania podwyższonym poziomem lęku w obliczu pojawiającychsię objawów oraz zagrożeń będących konsekwencjąchoroby. Zaobserwowane zależności potwierdzają hipotezę, żetemperament jest czynnikiem ryzyka w rozwoju zaburze

Drinking Parties and Learning: A Tale from Japan

Science.gov (United States)

Suchan, Jim

2007-01-01

In spring of 2006, the author spent a semester teaching undergraduate and graduate business and managerial communication courses at Chuo University, with intent to challenge his US-centric habits of mind about interaction and communication by immersing himself in a cultural and work environment fundamentally different from his own. Although…

Use of combined ion exchangers on the basis of KU-23 and KM-2p cation exchangers for purification of ammonium molybdate and tungstate solutions from phosphate, arsenate, and silicate impurities

International Nuclear Information System (INIS)

Blokhin, A.A.; Majorov, D.Yu.; Kopyrin, A.A.; Taushkanov, V.P.

2002-01-01

Using the Tracer technique ( 32 P) and elementary analysis, potentiality of using combined ionites on the basis of macroporous cation-exchange resins KU-23 or KM-2p and hydrated zirconium oxide for purification of concentrated solutions of ammonium molybdate and tungstate from phosphate-, arsenate-, and silicate-ions impurities was studied. High selectivity of the combined ionites towards impurity ions was ascertained, which permits reducing the content of impurities by a factor of 50-100 compared with the initial one [ru

Fiscal 1999 technical survey report. Basic survey of prioritized fields in Central Asia; 1999 nendo Chuo Asia chiiki juten bun'ya kiso chosa hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

For working out and proposing project themes optimum for the popularization of technologies for effectively utilizing energy in four countries in Central Asia (Kazakhstan, Uzbekistan, Turkmenistan, and Azerbaijan), basic surveys were conducted into their energy policies, trends of supply and demand, electric power industry, metal refining industry, and cement making industry. Common to the four nations were that they were rich in energy resources (natural gas, oil, and coal) but that they failed to utilize them effectively despite of an increase in the yields. Incomplete infrastructures and delay in the implementation of energy measures attracted attention. A higher priority was found attached to the upgrading of and investment in structures, renewal of facilities, and the intensification of management. The basic industries wanted the modernization of their facilities and processes. As for electric power consumption, Kazakhstan consumed 53.0-billion kWh, Uzbekistan 39.0-billion kWh, Turkmenistan 4.4-billion kWh, and Azerbaijan 16.4-billion kWh. In Uzbekistan, where the nonferrous metal refining industry led its national economy, the energy consumption rate was found to be quite high. There was a decrease in production in the cement making industry. (NEDO)

Extracted atmospheric impairments on earth-sky signal quality in tropical regions at Ku-band

Science.gov (United States)

Al-Saegh, Ali Mohammed; Sali, Aduwati; Mandeep, J. S.; Ismail, Alyani

2013-11-01

Atmospheric condition variations were shown to have a major effect on the earth sky signal quality at Ku band. Moreover, such variations increased in the tropical regions as compared to temperate areas due to their different weather parameters. With the increase of recent satellite communication technology applications throughout the tropical countries and lack of information regarding the atmospheric impairments analysis, simulation and mitigation techniques, there is an ever increasing need for extracting a unique and accurate performance of the signal quality effects during highly natural tropical weather impairments. This paper presents a new method developed for proper analysis with distinctive and highly realistic performance evaluation for signal quality during the atmospheric conditions variations in 14 tropical areas from the four continents analyzed based on actual measured parameters. The method implementation includes signal attenuation, carrier to noise ratio, symbol energy to noise ratio, and symbol error rate at different areas and different modulation schemes. Furthermore, for improvement in analysis in terms of covering more remarkable regions in tropics, the paper provides new measurements data with analysis for certain region in tropics used as a test bed and to add measurement data of such area to the world's data base for future researchers. The results show a significant investigation and performance observation in terms of weather impairments in tropical regions in general and each region in that area in particular regarding the signal attenuation and error rates accompanied for several transmission schemes.

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African Journals Online (AJOL)

J. S. Nyombo Luboya

P.O. Box 3006, Chuo Kikuu, Morogoro, TANZANIA. 2. Applied Human ... INTRODUCTION. Sweet potato (Ipomoea batatas L) is grown as a food crop in most parts of the world, ... diet and health status. .... Effect of drying on ascorbic acid, carotenoids, iron, calcium, oxalates and polyphenols ..... 1983; 10: 205-210. 28. Garman ...

Kinetics of destruction of KU-2 x 8 and AV-17 x 8 ion-exchange resins by the joint action of heating and irradiation

International Nuclear Information System (INIS)

Tulupov, P.E.; Butenko, T.Yu.

1982-01-01

Experimental data indicates a decrease of the exchange capacity of AV-17 x 8 (OH) resin in ion-exchange beds at various temperatures. Losses of exchange of the resin in hydroxyl form after 10,000h of continuous heating at 60, 70, and 80 0 C are 24, 44, and 78% respectively. Partial replacement of the hydroxyl by the salt form is accomplished by appreciable lowering of capacity losses. The trimethlyamine evolved during deamination of AV-17 x 8 anion exchange resin, and only methanol, enters the coolant. Under the influence of ionizing radiation in the active zone the methanol will be converted successively into formaldehyde, formic acid and CO 2 . Thus the maximum temperature of use of the hydroxyl form of te anion-exchange resin AV-17 x 8 (OH) in nuclear power plants can be raised to 70-75 0 C. Brief changes of temperature at 100 0 C do not cause any appreciable losses of exchange capacity either. Physicochemical properties of ion-exchange resins after use in nuclear power plants leads to excessive and unjustified restriction of their service life in the course of a single cycle. Joint action of heating and irradiation on AV-17 anion-exchange resin was studied. It was found that rate constants of the processes of thermal radiation decreases the exchange capacities of the resins in the temperature ranges of interest with relation to nuclear power plants. Calculation of the stability of KU-2 x 8 cation-exchange resin shows that even after a year of use at 100 0 C the loss of its exchange capacity is less than 0.1%. Therefore, under the conditions of use in a nuclear power plant KU-2 x 8 resin can be regarded as absolutely stable. The loss of exchange capacity of AV-17 x 8 resin in C1 form at 100 0 C is of similar magnitude

Pašvērtējuma, apmierinātības ar dzīvi un agresijas atšķirības pieaugušajiem ar un bez bērnībā pieredzētas vecāku šķiršanās

OpenAIRE

Ekarte, Marika

2013-01-01

Pētījuma mērķis ir noskaidrot vai pastāv atšķirības starp uzrādītajiem pašvērtējuma, apmierinātības ar dzīvi un agresijas rādītājiem respondentiem ar bērnībā pieredzētu vecāku šķiršanos un respondentiem bez vecāku šķiršanās pieredzes, kontrolējot dzimumu. Pētījuma mērķa noskaidrošanā pielietota pašvērtējuma aptauja (Rosenberg Self-Esteem Scale, RSE, Rosenberg, 1965), apmierinātības ar dzīvi aptauja (Satisfaction with life scales. Diener, 1985), agresijas līmeņa noteikšanai - agre...

Fiscal 1999 technical survey report. Basic survey of prioritized fields in Central Asia; 1999 nendo Chuo Asia chiiki juten bun'ya kiso chosa hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

For working out and proposing project themes optimum for the popularization of technologies for effectively utilizing energy in four countries in Central Asia (Kazakhstan, Uzbekistan, Turkmenistan, and Azerbaijan), basic surveys were conducted into their energy policies, trends of supply and demand, electric power industry, metal refining industry, and cement making industry. Common to the four nations were that they were rich in energy resources (natural gas, oil, and coal) but that they failed to utilize them effectively despite of an increase in the yields. Incomplete infrastructures and delay in the implementation of energy measures attracted attention. A higher priority was found attached to the upgrading of and investment in structures, renewal of facilities, and the intensification of management. The basic industries wanted the modernization of their facilities and processes. As for electric power consumption, Kazakhstan consumed 53.0-billion kWh, Uzbekistan 39.0-billion kWh, Turkmenistan 4.4-billion kWh, and Azerbaijan 16.4-billion kWh. In Uzbekistan, where the nonferrous metal refining industry led its national economy, the energy consumption rate was found to be quite high. There was a decrease in production in the cement making industry. (NEDO)

10- 12 gadus vecu bērnu ar atkārtotu nesekmību iemācītās bezpalīdzības saistība ar vecāku iemācīto bezpalīdzību

OpenAIRE

Lamstere, Jolanta

2006-01-01

Darbā tika pētīta iemācītās bezpalīdzības saistība bērniem ar vecāku iemācīto bezpalīdzību. Pētījuma gaitā tika izvirzīti pētījuma jautājumi: vai pastāv statistiski nozīmīgas sakarības starp iemācītās bezpalīdzības rādītājiem 10- 12 gadus veciem bērniem ar atkārtotu nesekmību un viņu vecāku iemācītās bezpalīdzības rādītājiem; vai pastāv statistiski nozīmīgas atšķirības starp iemācītās bezpalīdzības rādītājiem bērniem ar atkārtotu nesekmību un sekmīgiem bērniem; vai pastāv statistiski nozīmīga...

Microwave absorption in X and Ku band frequency of cotton fabric coated with Ni–Zn ferrite and carbon formulation in polyurethane matrix

International Nuclear Information System (INIS)

Gupta, K.K.; Abbas, S.M.; Goswami, T.H.; Abhyankar, A.C.

2014-01-01

The present study highlights various microwave properties, i.e. reflection, transmission, absorption and reflection loss, of the coated cotton fabric [formulation: Ni–Zn ferrite (Ni 0.5 Zn 0.5 Fe 2 O 4 ) and carbon black (acetylene black) at concentrations of 30, 40, 50, 60 and70 g of ferrite and 5 g carbon in each 100 ml polyurethane] evaluated at 8–18 GHz frequency. The uniform density of filling materials in coated fabrics (dotted marks in SEM micrograph) indicates homogeneous dispersion of conducting fillers in polyurethane and the density of filling material cluster increases with increase in ferrite concentration. SEM images also show uniform coating of conducting fillers/resin system over individual fibers and interweave spaces. The important parameters governing the microwave properties of coated fabrics i.e. permittivity and permeability, S-parameters, reflection loss, etc. were studied in a HVS free space microwave measurement system. The lossy character of coated fabric is found to increase with increase of ferrite content; the ferrite content decreases the impedance and increases the permittivity and permeability values. The 1.6–1.8 mm thick coated fabric sample (40 wt% ferrite, 3 wt% carbon and 57 wt% PU) has shown about 40% absorption, 20% transmission and 40% reflectance in X (8.2–12.4 GHz) and Ku (12–18 GHz) frequency bands. The reflection loss at 13.5 GHz has shown the highest peak value (22.5 dB) due to coated sample optical thickness equal to λ/4 and more than 7.5 dB in entire Ku band. Owing to its thin and flexible nature, the coated fabric can be used as apparel in protecting human being from hazardous microwaves and also as radar camouflage covering screen in defense. - Highlights: • Ni–Zn ferrite (Ni 0.5 Zn 0.5 Fe 2 O 4 ) with acetylene black found effective coating for microwave absorption. • Coating formulation containing 40 wt% ferrite, 3 wt% carbon and 57 wt% PU offered 40% absorption, 20% transmission and 40% reflection

Microwave absorption in X and Ku band frequency of cotton fabric coated with Ni–Zn ferrite and carbon formulation in polyurethane matrix

Energy Technology Data Exchange (ETDEWEB)

Gupta, K.K., E-mail: krishna62@rediffmail.com [Defence Materials and Stores Research and Development Establishment, Kanpur PO, GT Road, Kanpur 208013 (India); Abbas, S.M.; Goswami, T.H. [Defence Materials and Stores Research and Development Establishment, Kanpur PO, GT Road, Kanpur 208013 (India); Abhyankar, A.C. [Defence Institute of Advanced Technology( DIAT), Giri Nagar, Pune 411025 (India)

2014-08-01

The present study highlights various microwave properties, i.e. reflection, transmission, absorption and reflection loss, of the coated cotton fabric [formulation: Ni–Zn ferrite (Ni {sub 0.5}Zn{sub 0.5}Fe{sub 2}O{sub 4}) and carbon black (acetylene black) at concentrations of 30, 40, 50, 60 and70 g of ferrite and 5 g carbon in each 100 ml polyurethane] evaluated at 8–18 GHz frequency. The uniform density of filling materials in coated fabrics (dotted marks in SEM micrograph) indicates homogeneous dispersion of conducting fillers in polyurethane and the density of filling material cluster increases with increase in ferrite concentration. SEM images also show uniform coating of conducting fillers/resin system over individual fibers and interweave spaces. The important parameters governing the microwave properties of coated fabrics i.e. permittivity and permeability, S-parameters, reflection loss, etc. were studied in a HVS free space microwave measurement system. The lossy character of coated fabric is found to increase with increase of ferrite content; the ferrite content decreases the impedance and increases the permittivity and permeability values. The 1.6–1.8 mm thick coated fabric sample (40 wt% ferrite, 3 wt% carbon and 57 wt% PU) has shown about 40% absorption, 20% transmission and 40% reflectance in X (8.2–12.4 GHz) and Ku (12–18 GHz) frequency bands. The reflection loss at 13.5 GHz has shown the highest peak value (22.5 dB) due to coated sample optical thickness equal to λ/4 and more than 7.5 dB in entire Ku band. Owing to its thin and flexible nature, the coated fabric can be used as apparel in protecting human being from hazardous microwaves and also as radar camouflage covering screen in defense. - Highlights: • Ni–Zn ferrite (Ni{sub 0.5}Zn{sub 0.5}Fe{sub 2}O{sub 4}) with acetylene black found effective coating for microwave absorption. • Coating formulation containing 40 wt% ferrite, 3 wt% carbon and 57 wt% PU offered 40% absorption, 20

Pusaudžu tipiskās reakcijas pēc vecāku laulības šķiršanas.

OpenAIRE

Steļmaha, Elza

2011-01-01

Rezumējums Pētījuma „Pusaudžu depresija un uzvedības problēmas pēc vecāku laulības šķiršanas” mērķis bija noskaidrot kādas ir pusaudžu uzvedības un depresijas atšķirības starp šķirtajām un pilnajām ģimenēm.. Pētījuma papildus daļas mērķis bija noskaidrot kādas ir atšķirības depresijas izpausmēm un uzvedības problēmām, meitenēm un zēniem gan šķirto, gan pilno ģimeņu izlasē. Pētījumā piedalījās 60 respondenti vecuma grupā no 13-16 gadiem, 30 pusaudžiem vecāki ir šķiršanās procesā vai šķīr...

From Resident to President: The Sinuous Participation of Paraguayan Women in Politics and the Emergence of the Feminist Political Movement Kuña Pyrenda

Directory of Open Access Journals (Sweden)

Sarah Cerna Villarga

2015-07-01

Full Text Available Paraguayan women have transcended history as the mothers of the “Guarani breed”, as The Resident Woman Residentas, the social role of these has been undeniable, but in other scopes, such as the political area, its social and historical role is still invisible, because the policy has historically been considered a male activity. However, many women have contributed to the construction of the history of this nation, and although the asymmetries in the real access to power between men and women are notorious today, Paraguayan want power and will seek, as it was found in the last two presidential elections of 2008 and 2013. On this basis, this paper aims to describe what has been the impact of feminism in Paraguayan politics and, in turn, explain the factors that have influenced raid Kuña Pyrenda feminist movement in the elections of 2013.

Statistical analysis on dominating factor of pH in rain and snow sample. Investigation on water analysis from January, 1984 through December, 1986 sampled at Chuo-ku, Sapporo city

Energy Technology Data Exchange (ETDEWEB)

Watanabe, Norimoto; Kishi, Masami; Hayakawa, Osamu

1988-03-31

On the each samples of rain and snow collected in the City of Sapporo from January 1984 through December 1986, analyses were made in eleven ionic species, amount of rainfall, conductivity, ninhydrin-N, pH buffer, chemical oxygen demand (COD), and ultra violet absorbance. The pH of samples correlated to the logarithm of the concentration on each analysis except Na, NH/sub 4/, ninhydrin-N, and PO. Rainfall samples were divided into five respective pH range as follows: 5.0 or less, 5.0 to 5.5, 5.5 to 6.0, 6.0 to 6.5, and 6.5 or more. Equivalent amount of cation and anion, and cation/anion ratio increased in higher pH range. No significant correlation was found between the pH of the samples and the concentration of N and S oxides, nor between the hydrogen ion concentration precipitated amounts and the NO/sub 2/ and SO/sub 4/ precipitated amounts in pH range of 5.5 or less. The study yeilded the result that the increase of N and S oxides has little effect on the increase of H/sup +/. (8 figs, 6 tabs, 1 ref)

Achievement report for fiscal 1998. Optical tomographic system; 1998 nendo seika hokokusho. Hikari danso imaging system

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-05-01

Evaluations were given on spatial resolution and measurement time of an optical tomographic system by using the developed 64-channel time-resolved spectroscopy and an image reconstruction algorithm. With respect to the spatial resolution, the target value of 1 cm was verified from tomographic images of a phantom with a diameter of 10cm, simulating a neonate. The measurement time achieved 20 minutes, being one third of the target value. In installing the equipment at Hokkaido University, speeds of the optical switches and attenuators were increased to have reduced the measurement time to one minute. For installation at Kanagawa Rehabilitation Center, development has been made on a nano-second light pulser, whose average beam quantity has been increased to 40 times, and improvement has been given on the optical switches, the attenuators, and the indication software, by which the measurement time was decreased further by 30 seconds than that at Hokkaido University. In performing the clinical evaluation, the evaluation protocol resolved by the Experiment Evaluation Special Committee was submitted for deliberation at the Medical Welfare Device Clinical Evaluation Committee. Upon having been authorized by the Committee, the clinical evaluations were performed at Hokkaido University and the Kanagawa Rehabilitation Center. (NEDO)

Kinetic modelling and optimisation of antimicrobial compound production by Candida pyralidae KU736785 for control of Candida guilliermondii.

Science.gov (United States)

Mewa-Ngongang, Maxwell; du Plessis, Heinrich W; Hutchinson, Ucrecia F; Mekuto, Lukhanyo; Ntwampe, Seteno Ko

2017-06-01

Biological antimicrobial compounds from yeast can be used to address the critical need for safer preservatives in food, fruit and beverages. The inhibition of Candida guilliermondii, a common fermented beverage spoilage organism, was achieved using antimicrobial compounds produced by Candida pyralidae KU736785. The antimicrobial production system was modelled and optimised using response surface methodology, with 22.5 ℃ and pH of 5.0 being the optimum conditions. A new concept for quantifying spoilage organism inhibition was developed. The inhibition activity of the antimicrobial compounds was observed to be at a maximum after 17-23 h of fermentation, with C. pyralidae concentration being between 0.40 and 1.25 × 10 9 CFU ml -1 , while its maximum specific growth rate was 0.31-0.54 h -1 . The maximum inhibitory activity was between 0.19 and 1.08 l contaminated solidified media per millilitre of antimicrobial compound used. Furthermore, the antimicrobial compound formation rate was 0.037-0.086 l VZI ml -1 ACU h -1 , respectively. The response surface methodology analysis showed that the model developed sufficiently described the antimicrobial compound formation rate 1.08 l VZI ml -1 ACU, as 1.17 l VZI ml -1 ACU, predicted under the optimum production conditions.

Uticaj povećanja dometa na dinamičku stabilnost artiljerijskih raketa sa olučastim krilima / Influence of range extension on dynamic stability for artillery rockets with wrap around fins

Directory of Open Access Journals (Sweden)

Danilo Ćuk

2007-07-01

Full Text Available U radu su prikazani efekti povećanja dometa artiljerijske rakete sa olučastim krilima na njenu dinamičku stabilnost. Totalni impuls raketnog motora, uvećan za 50% i let rakete kroz slojeve atmosfere sa malom gustinom vazduha povećavaju domet rakete za više od 75% od nominalnog dometa. Međutim, let rakete kroz razređeni vazduh proizvodi teškoće koje se odnose na dinamičku stabilnost. Male promene bočnog momenta indukovanog napadnim uglom i Magnusovog momenta mogu proizvesti nestabilnost kretanja i nedozvoljeni rast napadnog ugla. Određene su tolerancije bočnog momenta za osnovnu i poboljšanu varijantu rakete radi sprečavanja nestabilnog leta. Analiziran je, takođe uticaj povećanja dometa na verovatna odstupanja rakete po dometu i pravcu. / This paper presents the effects of the range extension of an artillery rocket with wrap around fins on its dynamic stability. The increased total impulse of the rocket engine for 50% and flight through the atmosphere layers with low air density extend the range for more than 75% of the nominal range. However, the flight of the unguided rocket through the rarefied air produces difficulties related to the dynamic stability. The small changes in the side moment which is induced by the angle of attack and Magnus moment of the improved rocket can produce instabilities in flight and increasement the angle of attack. The tolerances of the side moment were determined for both basic and improved type of the rocket in order to prevent instable flight. The influence of the range extension on the probable errors in range and cross-range was analyzed as well.

A family of diverse Kunitz inhibitors from Echinococcus granulosus potentially involved in host-parasite cross-talk.

Science.gov (United States)

González, Silvia; Fló, Martín; Margenat, Mariana; Durán, Rosario; González-Sapienza, Gualberto; Graña, Martín; Parkinson, John; Maizels, Rick M; Salinas, Gustavo; Alvarez, Beatriz; Fernández, Cecilia

2009-09-17

The cestode Echinococcus granulosus, the agent of hydatidosis/echinococcosis, is remarkably well adapted to its definitive host. However, the molecular mechanisms underlying the successful establishment of larval worms (protoscoleces) in the dog duodenum are unknown. With the aim of identifying molecules participating in the E. granulosus-dog cross-talk, we surveyed the transcriptomes of protoscoleces and protoscoleces treated with pepsin at pH 2. This analysis identified a multigene family of secreted monodomain Kunitz proteins associated mostly with pepsin/H(+)-treated worms, suggesting that they play a role at the onset of infection. We present the relevant molecular features of eight members of the E. granulosus Kunitz family (EgKU-1 - EgKU-8). Although diverse, the family includes three pairs of close paralogs (EgKU-1/EgKU-4; EgKU-3/EgKU-8; EgKU-6/EgKU-7), which would be the products of recent gene duplications. In addition, we describe the purification of EgKU-1 and EgKU-8 from larval worms, and provide data indicating that some members of the family (notably, EgKU-3 and EgKU-8) are secreted by protoscoleces. Detailed kinetic studies with native EgKU-1 and EgKU-8 highlighted their functional diversity. Like most monodomain Kunitz proteins, EgKU-8 behaved as a slow, tight-binding inhibitor of serine proteases, with global inhibition constants (K(I) (*)) versus trypsins in the picomolar range. In sharp contrast, EgKU-1 did not inhibit any of the assayed peptidases. Interestingly, molecular modeling revealed structural elements associated with activity in Kunitz cation-channel blockers. We propose that this family of inhibitors has the potential to act at the E. granulosus-dog interface and interfere with host physiological processes at the initial stages of infection.

Rainfall measurement from the opportunistic use of an Earth–space link in the Ku band

Directory of Open Access Journals (Sweden)

L. Barthès

2013-08-01

Full Text Available The present study deals with the development of a low-cost microwave device devoted to the measurement of average rain rates observed along Earth–satellite links, the latter being characterized by a tropospheric path length of a few kilometres. The ground-based power measurements, which are made using the Ku-band television transmissions from several different geostationary satellites, are based on the principle that the atmospheric attenuation produced by rain encountered along each transmission path can be used to determine the path-averaged rain rate. This kind of device could be very useful in hilly areas where radar data are not available or in urban areas where such devices could be directly placed in homes by using residential TV antenna. The major difficulty encountered with this technique is that of retrieving rainfall characteristics in the presence of many other causes of received signal fluctuation, produced by atmospheric scintillation, variations in atmospheric composition (water vapour concentration, cloud water content or satellite transmission parameters (variations in emitted power, satellite pointing. In order to conduct a feasibility study with such a device, a measurement campaign was carried out over a period of five months close to Paris. The present paper proposes an algorithm based on an artificial neural network, used to identify dry and rainy periods and to model received signal variability resulting from effects not related to rain. When the altitude of the rain layer is taken into account, the rain attenuation can be inverted to obtain the path-averaged rain rate. The rainfall rates obtained from this process are compared with co-located rain gauges and radar measurements taken throughout the full duration of the campaign, and the most significant rainfall events are analysed.

Rainfall measurement from the opportunistic use of an Earth-space link in the Ku band

Science.gov (United States)

Barthès, L.; Mallet, C.

2013-08-01

The present study deals with the development of a low-cost microwave device devoted to the measurement of average rain rates observed along Earth-satellite links, the latter being characterized by a tropospheric path length of a few kilometres. The ground-based power measurements, which are made using the Ku-band television transmissions from several different geostationary satellites, are based on the principle that the atmospheric attenuation produced by rain encountered along each transmission path can be used to determine the path-averaged rain rate. This kind of device could be very useful in hilly areas where radar data are not available or in urban areas where such devices could be directly placed in homes by using residential TV antenna. The major difficulty encountered with this technique is that of retrieving rainfall characteristics in the presence of many other causes of received signal fluctuation, produced by atmospheric scintillation, variations in atmospheric composition (water vapour concentration, cloud water content) or satellite transmission parameters (variations in emitted power, satellite pointing). In order to conduct a feasibility study with such a device, a measurement campaign was carried out over a period of five months close to Paris. The present paper proposes an algorithm based on an artificial neural network, used to identify dry and rainy periods and to model received signal variability resulting from effects not related to rain. When the altitude of the rain layer is taken into account, the rain attenuation can be inverted to obtain the path-averaged rain rate. The rainfall rates obtained from this process are compared with co-located rain gauges and radar measurements taken throughout the full duration of the campaign, and the most significant rainfall events are analysed.

Estimating Snow Water Equivalent with Backscattering at X and Ku Band Based on Absorption Loss

Directory of Open Access Journals (Sweden)

Yurong Cui

2016-06-01

Full Text Available Snow water equivalent (SWE is a key parameter in the Earth’s energy budget and water cycle. It has been demonstrated that SWE can be retrieved using active microwave remote sensing from space. This necessitates the development of forward models that are capable of simulating the interactions of microwaves and the snow medium. Several proposed models have described snow as a collection of sphere- or ellipsoid-shaped ice particles embedded in air, while the microstructure of snow is, in reality, more complex. Natural snow usually forms a sintered structure following mechanical and thermal metamorphism processes. In this research, the bi-continuous vector radiative transfer (bi-continuous-VRT model, which firstly constructs snow microstructure more similar to real snow and then simulates the snow backscattering signal, is used as the forward model for SWE estimation. Based on this forward model, a parameterization scheme of snow volume backscattering is proposed. A relationship between snow optical thickness and single scattering albedo at X and Ku bands is established by analyzing the database generated from the bi-continuous-VRT model. A cost function with constraints is used to solve effective albedo and optical thickness, while the absorption part of optical thickness is obtained from these two parameters. SWE is estimated after a correction for physical temperature. The estimated SWE is correlated with the measured SWE with an acceptable accuracy. Validation against two-year measurements, using the SnowScat instrument from the Nordic Snow Radar Experiment (NoSREx, shows that the estimated SWE using the presented algorithm has a root mean square error (RMSE of 16.59 mm for the winter of 2009–2010 and 19.70 mm for the winter of 2010–2011.

The determinants of long-term care utilization and equity of access to care among older adults in Dong-Ku of Incheon Metropolitan city, South Korea.

Science.gov (United States)

Park, J M

2005-01-01

Under the current health care system, around three percent of the elderly remain uninsured. Based on the 2003 Dong-Ku Health Status Survey and the Aday and Andersen Access Framework, the present study examined the social and behavioral determinants of long-term care utilization and the extent to which equity in the use of long-term care services for the elderly has been achieved. The results indicate that universal health insurance system has not yielded a fully equitable distribution of services. Type of coverage and resource availability do not remain predictors of long-term care utilization. The data suggest that a universal health insurance system exists in South Korea with significant access problems for the population without insurance. Access differences also arise from obstacles in expanding the scope and level of plan benefits due to financial disparity among insurers. Health policy reforms must continue to concentrate on extending insurance coverage to the uninsured and establishing long-term insurance system for the elderly.

Effects of Snow/ Soil Interface on Microwave Backscatter of Terrestrial Snowpack at X- and Ku- Band

Science.gov (United States)

Kang, D. H.; Tan, S.; Zhu, J.; Gu, W.; Tsang, L.; Kim, E. J.

2017-12-01

Recent advances in monitoring and modeling capabilities to support remote sensing of terrestrial snow is encouraging to develop satellite mission concept in monitoring cold-region hydrological processes on global scales. However, it is still challenging to link back the active microwave backscattering signals to physical snowpack parameters. One of the limitations resides in the ignorance of the vegetation and soil conditions beneath the snowpack in the microwave scattering/ emission modeling and the snow water equivalent (SWE) retrieval algorithm. During the SnowEx 2017 winter campaign in Grand Mesa, CO, a particular effort has been made on comprehensive measurements of the underlying vegetation and soil characteristics from the snowpit measurements. Besides conducting standard snow core sampling, we have made additional protocols to record the background information beneath the snowpack. Recent works on active SWE retrieval algorithm using backscatters at X- (9.6 GHz) and Ku- (17.2 GHz) band suggest the significant signals from the background scattering characterization. The background scattering arising from the rough snow/ soil interface and the buried vegetation inside and beneath the snowpack modifies the sensitivity of the total backscatter to SWE. In this paper, we summarize the snow/ soil interface conditions as observed in the SnowEx campaign. We also develop standards for future in-situ snowpit measurements to include regular snow/ soil interface observations to accommodate the interpretation of microwave backscatter both for modeling and observation of microwave signatures. These observations first provide inputs to the microwave scattering models to predict the backscattering contribution from background, which is one of the key factors to be included to improve the SWE retrieval performance.

X ve Y Kuşağı: Hemşirelerin Meslek Dayanışması İle İş Doyumu Arasındaki İlişki

OpenAIRE

KARASU, Fatma; AYLAZ, Rukuye

2017-01-01

Amaç:Bu araştırma, X ve Y kuşağındaki hemşirelerin meslek dayanışması ile iş doyumuarasındaki ilişkisini belirlemek amacıyla yapılmıştır. Gereç ve Yöntem: Kesitsel nitelikteki araştırmanın evrenini devlethastanesinde çalışan hemşireler oluşturmuş, örneklem seçimine gidilmeyerekevrenin tamamı örnekleme dahil edilmiş ve 01.02.2017-01.03.2017 tarihleriarasında çalışmayı kabul eden toplamda 145 hemşireye ulaşılmıştır. Veri toplamaformunda, hemşirelerin sosyo-demografik özellikleri, Hemşirelerde M...

High-performance 16-way Ku-band radial power combiner based on the TE01-circular waveguide mode

Science.gov (United States)

Montejo-Garai, José R.; Saracho-Pantoja, Irene O.; Ruiz-Cruz, Jorge A.; Rebollar, Jesús M.

2018-03-01

This work presents a 16-way Ku-band radial power combiner for high power and high frequency applications, using the very low loss TE01 circular waveguide mode. The accomplished design shows an excellent performance: the experimental prototype has a return loss better than 30 dB, with a balance for the amplitudes of (±0.15 dB) and (±2.5°) for the phases, in a 16.7% fractional bandwidth (2 GHz centered at 12 GHz). For obtaining these outstanding specifications, required, for instance, in high-frequency amplification or on plasma systems, a rigorous step-by-step procedure is presented. First, a high-purity mode transducer has been designed, from the TE10 mode in the rectangular waveguide to the TE01 mode in the circular waveguide, with very high attenuation (>50 dB) for the other propagating and evanescent modes in the circular waveguide. This transducer has been manufactured and measured in a back-to-back configuration, validating the design process. Second, an E-plane 16-way radial power divider has been designed, where the power is coupled from the 16 non-reduced-height radial standard waveguides into the TE01 circular waveguide mode, improving the insertion loss response and removing the usual tapered transformers of previous designs limiting the power handling. Finally, both the transducer and the divider have been assembled to make the final radial combiner. The prototype has been carefully manufactured, showing very good agreement between the measurements and the full-wave simulations.

Topical steroid addiction in atopic dermatitis

OpenAIRE

Fukaya, Mototsugu; Sato, Kenji; Sato, Mitsuko; Kimata, Hajime; Fujisawa, Shigeki; Dozono, Haruhiko; Yoshizawa, Jun; Minaguchi, Satoko

2014-01-01

Mototsugu Fukaya,1 Kenji Sato,2 Mitsuko Sato,3 Hajime Kimata,4 Shigeki Fujisawa,5 Haruhiko Dozono,6 Jun Yoshizawa,7 Satoko Minaguchi8 1Tsurumai Kouen Clinic, Nagoya, 2Department of Dermatology, Hannan Chuo Hospital, Osaka, 3Sato Pediatric Clinic, Osaka, 4Kimata Hajime Clinic, Osaka, 5Fujisawa Dermatology Clinic, Tokyo, 6Dozono Medical House, Kagoshima, 7Yoshizawa Dermatology Clinic, Yokohama, 8Department of Dermatology, Kounosu Kyousei Hospital, Saitama, Japan Abstract: The American Academy ...

Marketing research on potential demands of photovoltaic systems. Taiyoko hatsuden system no senzai shijo chosa seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

1988-03-01

Applicable uses and the market size for photovoltaic (PV) systems were investigated taking into consideration the features and economics of such systems. As a result of computing representative usage examples together with the extent of potential demand for PV, 238 GW for the power source in private homes, 76 GW for green houses, 126 GW for sewage water treatment plants, 4 GW for schools and universities and others were estimated. For the greater number of PV systems already in actual use, non-cost factors acted as major determining factors when deciding on implementation. Promising uses (applicable ideas) seen from the standpoint of non-cost factors were studied in order to develop the PV system market and gain breakthrough for lowering future costs and increasing proliferation for such systems. When it comes to simultaneously decreasing costs and increasing demand for solar batteries in the coming days, it will be impossible to make an immediate transition from the present state of demand for public use and for special use to the final goal of demand as a power source in the home. There is a need for an intermediate demand that can bridge this gap. (13 figs, 9 tabs)

Achievement report for fiscal 1998. Esophageal vocalization aid system; 1998 nendo seika hokokusho. Shokudo hassei hojo sochi

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-05-01

Development has been made on element technologies for an esophageal vocalization aid system. With regard to the speaker, selection and trial production were performed on a speaker used for a phono-coupler to be used in coupling with a telephone transmitter. Performance not differing from that in the currently used telephone set was obtained in the overall characteristics evaluation using a dummy telephone circuitry. For the microphone, two kinds of hands-free microphones were fabricated on a trial basis. In order to develop pitch extraction and amplitude pitch conversion systems, pitch extraction performances were compared and discussed on the following five methods: the auto-correlation method, the Cepstram method, the average magnitude difference function (AMDF) method, the simplified inverse filter tracking (SIFT) method, and the time-domain excitation extractor using minimum perturbation operator (TEMPO) method. The hauling phenomenon, having come up as a problem in an auxiliary digital device, was analyzed to discuss methods for prevention thereof. In developing a voice/unvoiced distinction judgment method, a method using low domain power and high domain power was discussed. Development has been made on exclusive ICs, a voice analyzer, and the using feeling enhancing technology. In developing a total system, a digital unit incorporated esophageal vocalization aid system was developed and improved. (NEDO)

Gaistošo organisko savienojumu emisijas no kuņģa vēža audiem un to potenciālā bioloģiskā saistība

OpenAIRE

Aronsson, Dan Erik

2018-01-01

Gaistošā organiskā savienojuma (VOC) emisija, ko var novērot izelpā, ir strauji augoša diagnostikas metode audzēju izmeklēšanā. VOC ir savienojums, kam istabas temperatūrā ir augsts tvaika spiediens, kas veicina tā iztvaikošanu gāzes formā. Šo gāzi var noteikt cilvēka audos un šķidrumos.( Haick et al 2014) Galvenokārt ir pētīta VOC saistība ar plaušu vēzi. Taču aizvien vairāk tiek veikti pētījumi, lai noteiktu, kādas ir iespējas VOC pielietot arī kuņģa audzēja gadījumā. Tā kā VOC molekula sp...

A Ku band pulsed electron paramagnetic resonance spectrometer using an arbitrary waveform generator for quantum control experiments at millikelvin temperatures

Energy Technology Data Exchange (ETDEWEB)

Yap, Yung Szen, E-mail: yungszen@utm.my [Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka-shi, Osaka 560-8531 (Japan); Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor (Malaysia); Tabuchi, Yutaka [Research Center for Advanced Science and Technology (RCAST), The University of Tokyo, Meguro-ku, Tokyo 153-8904 (Japan); Negoro, Makoto; Kagawa, Akinori; Kitagawa, Masahiro, E-mail: kitagawa@ee.es.osaka-u.ac.jp [Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka-shi, Osaka 560-8531 (Japan)

2015-06-15

We present a 17 GHz (Ku band) arbitrary waveform pulsed electron paramagnetic resonance spectrometer for experiments down to millikelvin temperatures. The spectrometer is located at room temperature, while the resonator is placed either in a room temperature magnet or inside a cryogen-free dilution refrigerator; the operating temperature range of the dilution unit is from ca. 10 mK to 8 K. This combination provides the opportunity to perform quantum control experiments on electron spins in the pure-state regime. At 0.6 T, spin echo experiments were carried out using γ-irradiated quartz glass from 1 K to 12.3 mK. With decreasing temperatures, we observed an increase in spin echo signal intensities due to increasing spin polarizations, in accordance with theoretical predictions. Through experimental data fitting, thermal spin polarization at 100 mK was estimated to be at least 99%, which was almost pure state. Next, to demonstrate the ability to create arbitrary waveform pulses, we generate a shaped pulse by superposing three Gaussian pulses of different frequencies. The resulting pulse was able to selectively and coherently excite three different spin packets simultaneously—a useful ability for analyzing multi-spin system and for controlling a multi-qubit quantum computer. By applying this pulse to the inhomogeneously broadened sample, we obtain three well-resolved excitations at 8 K, 1 K, and 14 mK.

Problemas de los grupos *k(hi̭, *kṷ(hi̭, *t(hi̭ y *tṷ en griego

Directory of Open Access Journals (Sweden)

M.ª Luisa del Barrio Vega

1990-12-01

Full Text Available In this research the author studies a series of problems related to the evolution of the groups *k(hi̭, *kṷ(hi̭, *t(hi̭ and *tṷ in Greek. After a brief description of the palatalization process undergone by these groups and considering the different results from *t(hi̭ in the Ionic-Attic and Arcadian-Cypriot dialects which were swept twice by a wave of palatalization as well as the possible influence by analogy of the development undergone by *k(hi̭, the author examines in great depth the state of these groups at the time of the Mycenaean tablets analyzing the different values proposed for the series z- and s-. For z- an occlusive palatal and for s- an affricate articulation are considered the most likely ones. The author further suggests that the s- series followed an evolution parallel to that of the complex syllabograms of the type raz, roz, etc. Subsequently the author analyzes the graphic issues related to the evolution of the phonemic groups under study: The use of a special graphical notation in some Ionic and Pamphylian σσ/ττ in the dialects during the first millennium are due to the influence derived from the graphic representation of the state *ts. Finally the author suggests the Boeotian dialect as a starting point for the change *ts > tt, as in this dialect tt has a corresponding voiced series dd.

Functional simian immunodeficiency virus Gag-specific CD8+ intraepithelial lymphocytes in the mucosae of SIVmac251- or simian-human immunodeficiency virus KU2-infected macaques

International Nuclear Information System (INIS)

Stevceva, Liljana; Moniuszko, Marcin; Alvarez, Xavier; Lackner, Andrew A.; Franchini, Genoveffa

2004-01-01

The vaginal and rectal mucosae are the first line of cellular immune defense to sexually transmitted human immunodeficiency virus type 1 (HIV-1) entry. Thus, intraepithelial lymphocytes (IELs) may be important in the immune response to HIV infection. Here we investigated whether functional IELs in mucosal compartments could be visualized by direct staining with a tetrameric complex specific for the simian immunodeficiency virus (SIV) immunodominant Gag epitope in either separated IEL cells or tissues of macaques infected with SIVmac251. Of the 15 Mamu-A*01-positive macaques studied here, eight were chronically infected with either SIVmac251 or simian-human immunodeficiency virus (SHIV) KU2 and the remaining seven were exposed mucosally to SIVmac251 and sacrificed within 48 h to assess the local immune response. Gag-specific CD8+ T-cells were found in separated IELs from the rectum, colon, jejunum, and vagina of most infected animals. Direct staining of tetramers also revealed their presence in intact tissue. These Gag-specific IELs expressed the activation marker CD69 and produced IFN-γ, suggesting an active immune response in this locale

The Effect of the Unique Features of Y Generation on Organizational Commitment / Y Kuşağının Belirleyici Özelliklerinin Örgütsel Bağlılık Üzerindeki Etkisi

Directory of Open Access Journals (Sweden)

Mustafa Emre Civelek

2017-12-01

Full Text Available ABSTRACT In this research, the determinants of the generations and unique characteristics of Generation Y are examined and the effect of this unique characteristics on organizational commitment is investigated. In the introduction part of the research, different features separating the generations from each other were mentioned and the studies done in this subject were examined and the studies and findings of different researchers were evaluated. In the final part of the survey, there is the analysis result of the survey collected from overall Turkey from 8 corporations. Sample size of this research is 267.  In order to measure organizational commitment, the scale developed by Allen and Meyer was used (Allen & Meyer, 1993. In order to measure team working, the scale developed by Levi and Slem was used (Levi & Slem, 1995. In order to measure playful spirit at work, the scale developed by Berg was used (Berg, 2011. According to the test results, there is a positive and significant relationship between Workplace fun and Team Working and between Team Working and Organizational Commitment. Contrary to expectation, there is not directly relation between organizational commitment and workplace fun. But work place fun positively effects organizational commitment through team working indirectly. The results of this research are to confirm that employees will be more likely to work in teams if organization environments are established to make them happy and entertained, thereby increasing their organizational commitment. ÖZ Bu araştırmada Kuşakların belirleyici özellikleri incelenerek Y kuşağını diğer kuşaklardan ayıran özelliklerin neler olduğu ve bu özelliklerin örgütsel bağlılık üzerinde ne gibi bir etkisi olduğu araştırılmaktadır. Araştırmanın giriş kısmında kuşakları birbirinden ayrıştıran farklı özelliklere değinilmiş ve bu konuda yapılan çalışmalar incelenerek farklı araştırmacıların �

Trends spanning 36 years of nursing research

OpenAIRE

LEE Hyeyong; SHIMOTAKAHARA Rie; FUKADA Akimi; SHINBASHI Sumiko

2018-01-01

PURPOSE: The aim of demonstrating priorities for future research, this study draws on the strengths of text-mining analysis to analyze trends in nursing research in Japan over a 36-year period. METHODS: Documents published in the online version of the Igaku Chuo Zasshi (ICHUSHI) between 1980 and December 2015 were targeted for analysis. Tendencies and trends over time characterizing words used in the titles of all target research papers were analyzed, along with characteristic words. RESULTS:...

SERANGAN BENALU PADA BEBERAPA KELAS UMUR TANAMAN JATI DI WILAYAH HUTAN BKPH BEGAL, KPH NGAWI, JAWA TIMUR (An Attact of Parasitic Plant on Several Ages of Teak Plantation In Begal Forest Sub-District, Ngawi Forest District, East Java

Directory of Open Access Journals (Sweden)

Soewarno Hasanbahri

2014-10-01

The objectives of the study were to determine the relationship of teak plantation age class with the frequency and severity of teak trees due to an attack of parasitic plant (mistletoe, and parasitic plant distribution patterns horizontally. The sampling method in this study was the Multistage Sampling, i.e. at each age group classe (KU represented one compartment and each compartment made an individual sampling tree as much as 10 % for KU V and up (old age classes, 5 % for KU III – IV (middle age classes and 1 % for KU I - II (young age classes to the total trees populations. Age class relationship with the frequency and degree of tree damage due to an attack of parasitic plant were analyzed by correlation test formula and for the parasitic plant distribution were analysed by the Poisson distribution formula and the Binomial Test Statistically Reversed. The conclusions of this study that were found two species of parasitic plants of Dendrophthoe pentandra and Scurula parasitiaca of the Loranthaceae family members, sub-family Viscoidae. The frequency of teak trees that were attacted by parasitic plants ranged from 12.88 % for KU II and  followed by 15.55 % for KU I, 15.72 % for KU V, 18.06 % for KU IV and VI, up  to 19.73 % for KU III. The correlation between an attack of parasitic plants and the teak forest age classes have proven that significant relationship to the level of the proximal health. In another part, proved that age class does not have a significant relationship to the distal extent of damage. Nevertheless , the teak tree damage suffered an attack of parasitic plants were more prevalent on trees in the younger age classes. It was shown by a comparison of proximal and distal branches in each age class, namely: -23.81 cm for KU I, -1.56 cm for KU II, 14.66 cm for KU III, 24.13 cm for KU IV, 22.40 cm for KU V and 54.94 cm for KU VI. With the loss of future growth faced by teak trees in the young age classes become very vulnerable in the presence of

FY 1998 result report. Report on the results of the examinational research on the trend of technology development in the iron/steel industry; 1998 nendo seika hokokusho. Tekko sangyo no gijutsu kaihatsu doko nado ni kansuru chosa kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

The trend of the technology development in the Japanese iron/steel industry was compared with those in foreign countries and surveyed to increase the international competitive strength. From the viewpoints of technology, industry and goods, the iron/steel field was characterized based on the statistic data, and especially automobile use steel materials were selected such as cold rolling high tension steel sheet, surface treated steel sheet, and bearing sheet. The analysis of factors of competitive superiority was conducted, and the following were presented: measures for process continuation, and measures taken from the age of equipment and cost indexes. The paper also surveyed the trend of pig iron making/steel making technology in Asia. The present international technical competitiveness in the iron/steel industry in Japan, especially of automobile use steel sheet, is very high. This is because of the well-functioned demand-oriented development, and also as a result of the mutually influentially conducted equipment development such as process continuation and development of new goods, supposing the iron/steel continuous process and reduction in impurities. However, fears are the stagnation in the recent technical development strength and the saturation of new equipment. Moreover, the further heightening is needed of the international cost competitive strength of general-purpose products. (NEDO)

Report on the results of efforts for fiscal 1997. Development of technologies for creating high-quality crystal materials for low-loss power control devices; 1997 nendo seika hokokusho. Teisonshitsu denryoku seigyo soshiyo kohinshitsu kessho zairyo sosei gijutsu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

Basic technologies are developed for the manufacture of high-quality crystal materials large in diameter and homogeneous in property which will allow power control semiconductor devices to grow more sophisticated in function and to be lower in the loss they suffer. Physical properties of molten semiconductor and the like are measured accurately, which include surface tension, density, viscosity, electric conductivity, thermal conductivity, emissivity, diffusion coefficient, vapor pressure, heat capacity, contact angle, and solid solution equilibrium distribution coefficient. In computer-aided simulation, as in the previous year, simulation codes are developed for the analysis of flow, temperature distribution, and diffusion behavior in the gas phase; simulation codes are developed for the analysis of 3D unsteady thermal flows in the melt; and a main program is developed which governs experimental calculations. As for experiments in model Czochralski crucibles, small crucible are used in which experiments are conducted in the temperature range of normal to 200degC for the acquisition of experimental data for verification. Measured by use of the model crucibles are the temperature distribution in the bath, the surface flow speeds, and the flows inside the melt. 140 refs., 153 figs., 10 tabs.

Simulation study for ground-based Ku-band microwave observations of ozone and hydroxyl in the polar middle atmosphere

Science.gov (United States)

Newnham, David; Clilverd, Mark; Kosch, Michael; Verronen, Pekka

2017-04-01

Commercial satellite TV broadcasting is possible due to remarkable advances in microwave electronics, enabling weak signals transmitted over 36,000 km from geostationary orbit to be received by inexpensive rooftop dishes. The Ku band satellite frequencies (10.70-14.25 GHz) overlap microwave emissions from ozone (O3) at 11.072 GHz and hydroxyl radical (OH) at 13.44 GHz. These important chemical species in the polar middle atmosphere respond strongly to solar variability and, at high latitudes, geomagnetic activity associated with space weather. Atmospheric model calculations predict that energetic electron precipitation (EEP) driven by magnetospheric substorms produces large changes in polar mesospheric O3 and OH. The EEP typically peaks at geomagnetic latitudes ˜65˚ (e.g. Kilpisjärvi, Finland and Syowa station, Antarctica) and evolves rapidly with time eastwards and over the geomagnetic latitude range 60˚ -80˚ (e.g. reaching Halley, Antarctica). During the substorms OH can increase by more than 1000% at 64-84 km. The substorms leave footprints of 5-55% O3 loss lasting many hours of local time, with strong altitude and seasonal dependences. An atmospheric simulation and retrieval study is performed to determine the specification and design requirements for microwave radiometers capable of measuring O3 and OH profiles from Arctic and Antarctic locations using accessible satellite TV receiver technology. The proposed observations are highly applicable to studies of EEP, atmospheric dynamics, planetaryscale circulation, chemical transport, and the representation of these processes in polar and global climate models. They would provide a lowcost, reliable alternative to increasingly sparse satellite measurements, extending long-term data records and also providing "ground truth" calibration data.

Agrobacterium tumefaciens T-DNA Integration and Gene Targeting in Arabidopsis thaliana Non-Homologous End-Joining Mutants

Directory of Open Access Journals (Sweden)

Qi Jia

2012-01-01

Full Text Available In order to study the role of AtKu70 and AtKu80 in Agrobacterium-mediated transformation and gene targeting, plant lines with a T-DNA insertion in AtKu80 or AtKu70 genes were functionally characterized. Such plant lines lacked both subunits, indicating that heterodimer formation between AtKu70 and AtKu80 is needed for the stability of the proteins. Homozygous mutants were phenotypically indistinguishable from wild-type plants and were fertile. However, they were hypersensitive to the genotoxic agent bleomycin, resulting in more DSBs as quantified in comet assays. They had lower end-joining efficiency, suggesting that NHEJ is a critical pathway for DSB repair in plants. Both Atku mutants and a previously isolated Atmre11 mutant were impaired in Agrobacterium T-DNA integration via floral dip transformation, indicating that AtKu70, AtKu80, and AtMre11 play an important role in T-DNA integration in Arabidopsis. The frequency of gene targeting was not significantly increased in the Atku80 and Atku70 mutants, but it was increased at least 10-fold in the Atmre11 mutant compared with the wild type.

FY 1999 report on the results on analysis of protein functions; 1999 nendo tanpakushitsu kino kaiseki seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

This project is aimed at construction of the intellectual infrastructures for biotechnologies, in order to accelerate development of the Japanese technologies and activate their application to industries. Described herein are the FY 1999 results. These infrastructures are for functional analysis of protein which will be one of the key issues in genome analysis, and collection and analysis of biological information. This project includes a total of 9 research and development themes for four research categories: frequency analysis of gene expression (development of the gene expression profile database system for functional analysis of human genome, and analysis of the gene expression and protein functions by the ECA chip technology), function analysis by the biological model (high-performance analysis by the bio-project, database system for drug metabolizing enzymes, analysis of gene functions using mutant mice, and simple genome function analysis of murine individuals using the RNAi effect), protein expression (function validation of unknown human genes based on the useful biological model, and protein function analysis using multi-purpose destination vectors), and protein function prediction by the information science method. (NEDO)

Achievement report for fiscal 1982 on Sunshine Program. Coal energy; 1982 nendo sunshine keikaku seika hokokusho. Sekitan energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1982-07-01

This research program consists of (1) the solid catalyst-aided reforming of various petroleum based oils and heavy coal oils into Solvolysis solvents and the secondary hydrogenolysis, (2) a search for pre-treatment for improvement on the sub-bituminous coal liquefaction yield, and (3) a search for measures for improvement on the brown coal liquefaction yield. Studied in this fiscal year are (1) the high-temperature short-contact time liquefaction of brown coal, (2) the role of catalyst support for selective upgrading of SRC (solvent refined coal), and (3) reforming of thermal melting feature of low rank coal by preliminary deashing at room temperature. In relation with Item (1), it is found that the dehydrogenation or reaction to carbonization of molecules involved in hydrogen donation is structure-sensitive but that thermolysis of coal is accelerated under high-temperature conditions to allow transfer hydrogen to contribute uniformly. It is deemed also that matching is excellent between hydrogen transfer and thermolysis, that the solvent dissolution level is elevated, and that the reaction short in duration inhibits consecutive polymerization reaction. These are supposed to be effective in improving on the liquefaction yield and in rendering heavy constituents lighter. In relation with Item (2), it is found that the experimentally manufactured catalysts are effective in reducing the weight of heavy constituents. In relation with Item (3), a carbonization reforming method is experimentally implemented in which the ash that affects carbonization is further removed by a pre-treatment at room temperature. (NEDO)

Report on results of contract research. 'Research on MHD generation system'; MHD hatsuden system no kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1981-03-01

'Research on MHD generation system' was implemented by its expert committee in the electric joint study group, with the results of fiscal 1980 reported. This year, a detailed conceptual design was carried out on a coal fired MHD generation system, with points for the technological development concretely examined. In addition, investigation was conducted on the progress of MHD generation technology, development situation of other generation systems, state of energy resources, etc., in various foreign countries. In the conceptual design of the coal fired MHD generation plant, the system structure of a 2,000 MWt class commercial MHD generation plant was explained, as were the conceptual design of the structural elements and proposals for a 500 MWt class demonstration plant and an 100 MWt class experimental plant, for example. In the overseas trend of R and D on MHD generation, investigations were made concerning the U.S., Soviet Union, and China, with details compiled for such items as generation plants, combustors, generation channels, heat resisting materials, superconducting magnets, heat exchangers, seed slags, inverters, boilers and environments, and commercial plants. (NEDO)

Achievement report for fiscal 1998. Multimedia system for the disabled; 1998 nendo seika hokokusho. Shogaisha taio multimedia system

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-05-01

Development has been made on the element technologies for a multimedia system for the disabled. In developing the non-visual graphical user interface (GUI) access system, a non-visual access system to enable the visually disabled to access a GUI system by utilizing sound and tactile devices was developed. In developing the three-dimensional sound utilizing information providing system, a system was developed, in which information on screen arrangement of the GUI in a personal computer is presented as spatial sound positions by using the three-dimensional sound control technology. The system can be operated non-visually using a mouse by controlling movements of a cursor. The current fiscal year has improved three-dimensional sound producing devices, three-dimensional sound interface, and how to handle their applications. User evaluations were also performed. Functions were expanded and improved in a system and an optical media reader that enable the visually disabled person to read printed papers by himself. Development was made also on a function to link the system with a total system. (NEDO)

Report on results of contract research. 'Research on MHD generation system'; MHD hatsuden system no kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1982-03-01

'Research on MHD generation system' was implemented by its expert committee in the electric joint study group, with the results of fiscal 1981 reported. This year, technological reexamination was conducted for a 2,000 MWt commercial MHD generation plant, with evaluation carried out on the cost performance including the construction and operation cost. In addition, for the purpose of intermediate R and D towards the practicability, examination was also conducted on a system structure, concrete specifications of component element, cost of R and D including operation expenses for example, concerning an 100 MWt class experimental plant and a 500 MWt class plant. In the investigation of the overseas trend, information was summarized in detail on the experimental devices, combustors, generation channels, electrode materials, electrode phenomena, theoretical analyses, seeds, slag, component equipment, instrumental technologies, conceptual designs of generation plant, commercial plant, etc., in Soviet Union, China, Holland, India and EPRI, on the basis of the materials from the 19th MHD symposium held in UTSI and from the coal MHD specialist conference held in Sydney. (NEDO)

Report on results of contract research. 'Research on MHD generation system'; MHD hatsuden system no kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1983-03-01

'Research on MHD generation system' was implemented by its expert committee in the electric joint study group, with the results of fiscal 1982 reported. This year is the final year of this research; therefore, reexamination was made from the practical standpoint on the R and D of coal fired MHD generation, evaluating the present technological level as well as clarifying the development procedures, with proposals made as to the R and D from now on. The present technological level in the practicability is still in the basic stage, where the essential problem is the development of combustors, air heaters and generation channels in particular. An examination was conducted for the problems, procedures and period of the development for each component equipment, with the results provided as materials for studying the path to the experimental plant of 100 MW heat input. In the method of proceeding with the R and D in the future, it was decided as essential, in order to enter the stage of the experimental plant from the element technology development, that R and D is carried out on the unestablished component technology, making sure that no excessive risk is taken in scaling up to the next stage. (NEDO)

Multi-Satellite MIMO Communications at Ku-Band and Above: Investigations on Spatial Multiplexing for Capacity Improvement and Selection Diversity for Interference Mitigation

Directory of Open Access Journals (Sweden)

Liolis Konstantinos P

2007-01-01

Full Text Available This paper investigates the applicability of multiple-input multiple-output (MIMO technology to satellite communications at the Ku-band and above. After introducing the possible diversity sources to form a MIMO matrix channel in a satellite environment, particular emphasis is put on satellite diversity. Two specific different topics from the field of MIMO technology applications to satellite communications at these frequencies are further analyzed: (i capacity improvement achieved by MIMO spatial multiplexing systems and (ii interference mitigation achieved by MIMO diversity systems employing receive antenna selection. In the first case, a single-user capacity analysis of a satellite MIMO spatial multiplexing system is presented and a useful analytical closed form expression is derived for the outage capacity achieved. In the second case, a satellite MIMO diversity system with receive antenna selection is considered, adjacent satellite cochannel interference on its forward link is studied and an analytical model predicting the interference mitigation achieved is presented. In both cases, an appropriate physical MIMO channel model is assumed which takes into account the propagation phenomena related to the frequencies of interest, such as clear line-of-sight operation, high antenna directivity, the effect of rain fading, and the slant path lengths difference. Useful numerical results obtained through the analytical expressions derived are presented to compare the performance of multi-satellite MIMO systems to relevant single-input single-output (SISO ones.

The University of Kansas High-Throughput Screening laboratory. Part I: meeting drug-discovery needs in the heartland of America with entrepreneurial flair.

Science.gov (United States)

McDonald, Peter R; Roy, Anuradha; Chaguturu, Rathnam

2011-05-01

The University of Kansas High-Throughput Screening (KU HTS) core is a state-of-the-art drug-discovery facility with an entrepreneurial open-service policy, which provides centralized resources supporting public- and private-sector research initiatives. The KU HTS core applies pharmaceutical industry project-management principles in an academic setting by bringing together multidisciplinary teams to fill critical scientific and technology gaps, using an experienced team of industry-trained researchers and project managers. The KU HTS proactively engages in supporting grant applications for extramural funding, intellectual-property management and technology transfer. The KU HTS staff further provides educational opportunities for the KU faculty and students to learn cutting-edge technologies in drug-discovery platforms through seminars, workshops, internships and course teaching. This is the first instalment of a two-part contribution from the KU HTS laboratory.

KUŞAKLARIN SİYASAL İLETİŞİM KÜLTÜR VE LİDERLİK AÇISINDAN DEĞERLENDİRİLMESİ

OpenAIRE

GÖKTAŞ, Pınar; ÇARIKÇI, İlker Hüseyin

2018-01-01

Kuşaklararası farklılıklar; bireylerin iletişim tarzını vearzu edilen liderlik tarzını etkilemektedir. Tipik kuşak çatışmalarının çoğuiletişimsizlikten kaynaklanmaktadır. Bu sorunu çözmek için hedef kitleye yönelikiletişim tarzı seçilmelidir. Liderlik tarzı açısından ise kuşaklar inandırıcı vegüvenilir bir lider arzusu içindedir. Araştırma kapsamında aynı zamandaaralarındaki farka da değinebilmek adına sessiz kuşak, patlama kuşağı, X, Y ve Zkuşaklarına yer verilmiştir. Bu bağlamda kuşaklar il...

Nuclear survivin and its relationship to DNA damage repair genes in non-small cell lung cancer investigated using tissue array.

Directory of Open Access Journals (Sweden)

Songliu Hu

Full Text Available To investigate the predictive role and association of nuclear survivin and the DNA double-strand breaks repair genes in non-small cell lung cancer (NSCLC: DNA-dependent protein kinase catalytic subunit (DNA-PKcs, Ku heterodimeric regulatory complex 70-KD subunit (Ku70 and ataxia-telangiectasia mutated (ATM.The protein expression of nuclear survivin, DNA-PKcs, Ku70 and ATM were investigated using immunohistochemistry in tumors from 256 patients with surgically resected NSCLC. Furthermore, we analyzed the correlation between the expression of nuclear survivin, DNA-PKcs, Ku70 and ATM. Univariate and multivariate analyses were performed to determine the prognostic factors that inuenced the overall survival and disease-free survival of NSCLC.The expression of nuclear survivin, DNA-PKcs, Ku70 and ATM was significantly higher in tumor tissues than in normal tissues. By dichotomizing the specimens as expressing low or high levels of nuclear survivin, nuclear survivin correlated significantly with the pathologic stage (P = 0.009 and lymph node status (P = 0.004. The nuclear survivin levels were an independent prognostic factor for both the overall survival and the disease-free survival in univariate and multivariate analyses. Patients with low Ku70 and DNA-PKcs expression had a greater benefit from radiotherapy than patients with high expression of Ku70 (P = 0.012 and DNA-PKcs (P = 0.02. Nuclear survivin expression positively correlated with DNA-PKcs (P<0.001 and Ku70 expression (P<0.001.Nuclear survivin may be a prognostic factor for overall survival in patients with resected stage I-IIIA NSCLC. DNA-PKcs and Ku70 could predict the effect of radiotherapy in patients with NSCLC. Nuclear survivin may also stimulates DNA double-strand breaks repair by its interaction with DNA-PKcs and Ku70.

The Comparison of Canopy Height Profiles Extracted from Ku-band Profile Radar Waveforms and LiDAR Data

Directory of Open Access Journals (Sweden)

Hui Zhou

2018-05-01

Full Text Available An airborne Ku-band frequency-modulated continuous waveform (FM-CW profiling radar, Tomoradar, records the backscatter signal from the canopy surface and the underlying ground in the southern boreal forest zone of Finland. The recorded waveforms are transformed into canopy height profiles (CHP with a similar methodology utilized in large-footprint light detection and ranging (LiDAR. The point cloud data simultaneously collected by a Velodyne® VLP-16 LiDAR on-board the same platform represent the frequency of discrete returns, which are also applied to the extraction of the CHP by calculating the gap probability and incremental distribution. To thoroughly explore the relationships of the CHP derived from Tomoradar waveforms and LiDAR data we utilized the effective waveforms of one-stripe field measurements and comparison them with four indicators, including the correlation coefficient, the root-mean-square error (RMSE of the difference, and the coefficient of determination and the RMSE of residuals of linear regression. By setting the Tomoradar footprint as 20 degrees to contain over 95% of the transmitting energy of the main lobe, the results show that 88.17% of the CHPs derived from Tomoradar waveforms correlated well with those from the LiDAR data; 98% of the RMSEs of the difference ranged between 0.002 and 0.01; 79.89% of the coefficients of determination were larger than 0.5; and 98.89% of the RMSEs of the residuals ranged from 0.001 to 0.01. Based on the investigations, we discovered that the locations of the greatest CHP derived from the Tomoradar were obviously deeper than those from the LiDAR, which indicated that the Tomoradar microwave signal had a stronger penetration capability than the LiDAR signal. Meanwhile, there are smaller differences (the average RMSEs of differences is only 0.0042 when the total canopy closure is less than 0.5 and better linear regression results in an area with a relatively open canopy than with a denser

Fiscal 1997 report on the results of the R and D on the invention of high-level combustion technology using the microgravity environment; 1997 nendo seika hokokusho (bisho juryoku kankyo wo riyoshita kodo nensho gijutsu soshutsu ni kansuru kenkyu kaihatsu) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

For the purpose of achieving both the diversification of energy resources and the reduction in environmental pollutants in fuel exhaust gases, the paper conducted elucidation of combustion phenomena using microgravity facilities and research on combustors which make high-level combustion possible. In the experimental study using microgravity experimental facilities, conducted were evaluation experiments on combustion/evaporation of fuel droplets and droplet groups, elucidation/evaluation experiments of combustion characteristics of high-density fuels, evaluation experiments of flammability limit, and elucidation/evaluation experiments of emission mechanisms of NOx, etc. Analysis/evaluation of the data obtained and elucidation of the combustion mechanism were conducted. Further, by applying the results of the microgravity experiment, the high-level combustor experimental facilities were fabricated, and validation tests were started. In the international joint research with US`s NASA, the following were conducted using JAMIC`s facilities and NASA`s 2.2 second drop tower: study of mutual interference of droplet series combustion, study of binary system fuel droplet series combustion, study of combustion characteristics of solid fuels, study of behavior of flames around the lean flammable limit, etc. 151 refs., 253 figs., 7 tabs.

Pilot testing of the Czech versions of the Coaching Behavior Assessment Questionnaire structure [Pilotní testování struktury české verze dotazníku Coaching Behavior Assessment Questionnaire

Directory of Open Access Journals (Sweden)

Martin Musálek

2012-03-01

Martin a Kelly Barnes vytvořili v roce 1999 dotazník Coaching Behavior Assessment Questionnaire, který vychází z CBAS (Smith a kol., 1977 a LSS (Chelladurai & Saleh, 1978, 1980. V současné době není v České republice podobný diagnostický nástroj pro hodnocení chování trenéra. CÍLE: Cílem této pilotní studie bylo přeložit a ohodnotit strukturu dotazníku CBAQ metodou strukturálního modelování pro populaci dětí ve věku 10 – 16 let. METODIKA: Pro účel překladu dotazníku byla použita modifikovaná metoda přímého překladu (Behling & Law, 2000. Dotazník vyplnilo 100 účastníků ve věku 10 – 16 let, 63 děvčat (průměrný věk 13,02; SD = 2,10, 37 chlapců (průměrný věk 13,27; SD = 1,37. Pro analýzu dat byla použita exploratorní a konfirmační faktorová analýza. VÝSLEDKY: Původní model byl testován za účelem ověření struktury a diagnostické kvality tohoto nástroje. Výsledky původního 12-faktorového modelu CBAQ ukázaly slabý fit modelu s podprůměrnými hodnotami indexů fitu RMSEA = 0,082, CFI = 0,600 a také nízké koeficienty generické reliability. Z tohoto důvodu jsme se rozhodli pro re-analýzu dat, ze které byla vytvořena modifikovaná 5-faktorová struktura CBAQ. Tento 5-faktorový model s omezeným počtem položek ukázal nejlepší hodnoty indexů fitu RMSEA = 0,056; CFI = 0.964 a také akceptovatelné hodnoty generické reliability. ZÁVĚRY: Tato studie měla pilotní charakter, uvědomujeme si proto, že snížení velkého počtu položek u této modifikované verze vedlo ke ztrátě informace. Přesto se domníváme, že by tato finální 5-faktorová verze s 15 položkami mohla tvořit základ pro budoucí připravovanou plně standardizovanou českou verzi CBAQ.

Mechanický návrh průběžného zásobníku příze a jeho realizace

OpenAIRE

Klusoň, Vladimír

2014-01-01

Práce se zabývá vývojem zařízení Průběžného zásobníku příze pro textilní stroj. Průběžný zásobník přízeobsahuje poháněný navíjecí válec s rotačním kompenzátorem napětí příze, flyerem. Poháněný otočný zásobník je spřažen s prvním pohonem tvořeným elektrickým motorem a rotační kompenzátor je spřažen s druhým pohonem tvořeným elektrickým motorem, přičemž oba motory jsou napojeny na řídicí systém. Zařízení Průběžný zásobník příze efektivně zvyšuje stupeň automatizace textilního stroje. This th...

Vecāku prombūtnes un klātesošā vecāka audzināšanas pieejas saistība ar pusaudžu depresijas pazīmēm

OpenAIRE

Ločmele, Renāte

2014-01-01

Pētījuma mērķis bija noskaidrot, kādas ir saistības starp vecāku prombūtni, klātesošā vecāka audzināšanas pieeju un pusaudžu depresijas pazīmēm. Pētījumā piedalījās 62 respondenti – 37 meitenes un 25 zēni vecumā no 11 līdz 16 gadiem. Respondentu vidējais vecums bija 14 gadi. Tie tika sadalīti divās grupās: vienā grupā bija pusaudži, kuriem viens no vecākiem atrodas prombūtnē (n=31), otrā grupā bija pusaudži, kas dzīvo kopā ar abiem vecākiem ( n=31). Datu ievākšanai tika izmantotas sekojoš...

Ku to V-band 4-bit MEMS phase shifter bank using high isolation SP4T switches and DMTL structures

Science.gov (United States)

Dey, Sukomal; Koul, Shiban K.; Poddar, Ajay K.; Rohde, Ulrich L.

2017-10-01

This work presents a micro-electro-mechanical system (MEMS) based on a wide-band 4-bit phase shifter using two back-to-back single-pole-four-throw (SP4T) switches and four different distributed MEMS transmission line (DMTL) structures that are implemented on 635 µm alumina substrate using surface micromachining process. An SP4T switch is designed with a series-shunt configuration and it demonstrates an average return loss of  >17 dB, an insertion loss of  28 dB up to 60 GHz. A maximum area of the SP4T switch is ~0.76 mm2. Single-pole-single-throw and SP4T switches are capable of handling 1 W of radio frequency (RF) power up to  >100 million cycles at 25° C; they can even sustained up to  >70 million cycles with 1 W at 85 °C. The proposed wide-band phase shifter works at 17 GHz (Ku-band), 25 GHz (K-band), 35 GHz (Ka-band) and 60 GHz (V-band) frequencies. Finally,a 4-bit phase shifter demonstrates an average insertion loss of  10 dB and maximum phase error of ~3.8° at 60 GHz frequency over 500 MHz bandwidth. Total area of the fabricated device is ~11 mm2. In addition, the proposed device works well up to  >107 cycles with 1 W of RF power. To the best of the author’s knowledge, this is the best reported wide-band MEMS 4-bit phase shifter in the literature that works with a constant resolution.

Hard X-Ray Properties of the Merging Cluster Abell 3667 as Observed with Suzaku

Science.gov (United States)

2008-08-01

3667 as Observed with Suzaku Kazuhiro NAKAZAWA Department of Physics, The University of Tokyo, 7-3-1 Hongo , Bunkyo-ku, Tokyo 113-0033 nakazawa...of Tokyo, 7-3-1 Hongo , Bunkyo-ku, Tokyo 113-0033 Kazuo MAKISHIMA Department of Physics, The University of Tokyo, 7-3-1 Hongo , Bunkyo-ku, Tokyo 113

Summarized achievement report on the Sunshine Project in fiscal 1980 (Hydrogen energy); 1980 nendo seika hokokusho gaiyoshu. Suiso energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1981-04-01

This paper summarizes the achievement report on the Sunshine Project in fiscal 1980 for hydrogen energy research. In hydrogen manufacturing using the electrolytic process, improvements were made on membranes and electrodes. Solid electrolyte electrolysis is also under research. Researches are continued on reaction, separating operation, and device materials for the iodine system cycle in the thermo-chemical method. In the iron system cycle, a reaction experimenting equipment was fabricated on the trial basis, and tests and evaluation were performed on the material and heat balances. In the mixed system cycle, researches on the light irradiation electrolytic process were continued, whereas the light collecting rate was raised by using a lens to increase light intensity, having enhanced successfully the reaction rate to 60 to 80%. A heat diffusion column for HI decomposition and separation (hydrogen acquisition) was discussed in terms of chemical engineering. Development works are continued on metal hydrides for hydrogen transportation, and durability tests are also being performed. Same applies to hydrogen storage. A model burner was fabricated on the trial basis, and catalytic combustion was studied as development of a combustion technology that matches the requirements for safe hydrogen combustion and suppression of NOx emission. Searches were continued on catalysts and solid electrolyte materials for fuel cells. Thin film sold electrolyte fuel cells constructed by using the evaporation process are also being studied. The paper also describes measures for hydrogen safety assurance and researches on energy systems. (NEDO)

Fiscal 1997 achievement report. Research and development of synergy ceramics; 1997 nendo synergy ceramics no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

Research and development is conducted on two subjects, that is, 1) hyper organized structure control technology and 2) structural element control technology. In addition, joint research and development is conducted on the creation of new materials by hyper organized structure controlling, hyper organized structure controlling for ceramics by a structurization reaction process, designing of precursors to ceramics, and the hyper organized structure control for ceramics by nanostructure process control. The joint research and development endeavors further deal with re-entrusted projects which involve researches on sintered structure control by powdery particulate structure control; dynamic process of synergy ceramics; oxynitride liquids, glasses, and glass-ceramics; and multifunctional ceramic laminates for engineering applications. Under subject 1), researches are made on the development of precursors into ceramics by utilizing chemical reactions of organic metal compounds, and analyses are conducted into the effects, exerted by the molecular structures of precursors and the conditions of a reaction for their development into ceramics, on the microstructures and various properties of the ceramics to be composed. Under subject 2), high strength, great hardness, and high resistance to wear are realized by allowing the precipitation of nano-particulates in crystals of a fine and very compact sintered body of alumina. (NEDO)

Report of results of contract research. 'Research on magneto hydrodynamic (MHD) generation'; MHD hatsuden system no kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1980-03-01

Examination was conducted in detail on an MHD generation system by coal combustion, with the results reported. Concerning a gas table calculation program in coal combustion, it was prepared assuming 100% slag removal ratio in the combustor as the primary approximation. A combustor for MHD generation needs to efficiently burn fuel using high temperature pre-heated air as the oxidant, to fully dissociate/electrolytically dissociate seed, and to supply to the generation channel a high speed combustion gas plasma having a high electrical conductivity which is required for MHD generation. This year, an examination was conducted on technological problems in burning coal in an MHD combustor. As for the NOx elimination system in an MHD generation plant, an examination was made if the method studied so far in MHD generation using heavy oil as the fuel is applicable to coal. Also investigated and reviewed were various characteristics, change in physical properties, recovery method, etc., in a mixed state of seed and slag in the case of coal combustion MHD. (NEDO)

Achievement report on developing inverse manufacturing system in fiscal 1998; 1998 nendo inverse manufacturing system no kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

Research and development has been performed on a circulating type production system, or an inverse manufacturing system, to minimize the environmental load in the entire life cycle of industrial products from design to manufacturing, maintenance, recovery, and re-utilization. In developing the recycling information system, discussion and extraction were executed on the data to be exchanged between manufacturers and users in the inverse society. A new individual parts history control system was developed. In developing the recycling processing system, a prototype system was evaluated by using hypothetical data, wherein the possibility of improving the recycling processing efficiency and reducing the cost was verified. In addition, trial design was made on a recycling processing plant in consideration of the cost effect to get the future plant image. In fabricating the inverse manufacturing products, a prototype concept model was made for information terminal devices. Development was performed on such elementary technologies as the easy-to-disintegrate structure technology, self-integration connecting structure and environmental hysteresis detection system. (NEDO)

Fiscal 2000 achievement report. Welfare technosystem research and development (Mizusawa); 2000 nendo walfare technosystem kenkyu kaihatsu (Mizusawa) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

For assisting aged people, healthy and living alone, in living without help, activities were conducted for (1) an electrical transmission experiment connecting residences (two, inhabited for the experiment) and a remote location (Techno-House) and (2) the development of a home server equipped with an electrocardiograph. Under item (1), data collected by sensors (occupancy sensor, open/close sensor, flame sensor, waterflow sensor) installed at the residences occupied by aged persons living alone were forwarded to Techno-House via CATV (cable television) and analyzed for the study of elderly people's behavior and daily life pattern. It was found that using this system the behavior of elderly people living alone might be detected even in the absence of their intention to report or communicate. Under item (2), an Ni-based electrode sheet was fabricated and installed in a bed and, via the sheet and a CATV channel, cardiographic waveforms were transmitted to Techno-House where the data were put into an analyzer for the examination of the elderly person's physical conditions. (NEDO)

Fiscal 2000 achievement report. Welfare technosystem research and development (Sapporo); 2000 nendo Welfare technosystem kenkyu kaihatsu (Sapporo) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

Activities are conducted on system technologies for ensuring comfortable living circumstances and thereby helping aged or handicapped people live independently in snowy cold regions. The research and development efforts center about the three fields of (1) in-house systems to help aged or handicapped people live an independent life, (2) in-home care equipment systems suitable for advanced welfare oriented residences, and (3) user interface controllers to improve on operating sensation in a motor driven wheelchair. In field (1), studies involve the development of low floor residences for snowy cold regions, improvement on entrances for independent residences helping the inhabitants lead an independent life, and functionally excellent designs for handrails for steps, stairs, or bathrooms. In field (2), surveys are conducted concerning design improvements and their acceptability relative to a universal welfare equipment controller, optimum sound that welfare equipment alarms should produce, and transfer devices. In field (3), studies are made about motor driven wheelchair controllers. (NEDO)

Fiscal 2000 achievement report. Welfare technosystem research and development (Niihama); 2000 nendo walfare technosystem kenkyu kaihatsu (Niihama) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

Efforts are made to develop welfare equipment systems for helping people needing care lead an independent life. In collecting data on the transfer of patients and carrying out research and development in this connection, activities are conducted to develop (1) a transfer wheelchair and a transfer aiding wheelchair, (2) a simplified transfer type toilet, and (3) a tilt mechanism installable on a standard wheelchair. Under item (1), a wheelchair and an auxiliary wheelchair enabling the transfer of a person who cannot move without help between a wheelchair and an automobile are experimentally built. Under item (2), a simplified transfer type toilet to which a single attendant can easily guide the patient from the bed is developed. Under item (3), a tilt mechanism is developed, installable on a wheelchair to recline the same for the aged person to assume a comfortable attitude. In the development of a portable character reading device, a finger tip reader was experimentally manufactured for reading Japanese characters. (NEDO)

Fiscal 2000 achievement report. Welfare technosystem research and development (Ube); 2000 nendo walfare technosystem kenkyu kaihatsu (Ube) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

Efforts continue to develop welfare nursing equipment for helping persons in need of nursing care or handicapped persons who live an independent life. Efforts are exerted in three fields for developing (1) a motor driven wheelchair in which the occupant feels as if driving a car, (2) an online cardiac function monitoring system using a cardiac sound sensor, (3) an automatic pressure belt for patients of postural low blood pressure, (4) and a seated position aiding and stabilizing device for workers with legs not fully useful. In field (1), the steering wheel, brake, and driving system are contrived so that the occupant may easily drive the wheelchair as if in an automobile driver's seat. In field (2), a device is developed to monitor and examine the heart rate and respiration rate, for which a cardiac sensor has to be installed on the water bed or an air mattress. In field (3), a computer controlled automatic pressure belt is developed for patients of myelopathy or cerebrovascular disease. In field (4), a work assisting device is developed to help seated workers with leg troubles. (NEDO)

Fiscal 1999 achievement report. Research and development of synergy ceramics; 1999 nendo synergy ceramics no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

Studies are conducted on three subjects, which are the development of 1) high-temperature energy materials technology, 2) ultrasophisticated materials technology, and 3) high-performance active materials technology. Under subject 1), high-strength porous materials are to be developed, which are resistant to heat and corrosion, tolerant of damage and deformation, and light in weight. In addition, relative to fluid transmission, materials are to be developed, which are highly resistant to heat, corrosion, and thermal impact, and capable of separating and removing particulates of powder dust. Under subject 2), efforts are under way to develop two technologies, related to materials creation and their application. The former technology involves the development of a process of simultaneously equipping materials with high wear resistance, desired friction factors, and desired mechanical and thermal characteristics; a process of equipping them with a trace damage detecting function; and a process of simultaneously equipping them with functions of restoration from accidents and of self-lubrication. Relative to the latter technology, efforts are conducted, in coordination with the endeavors for the above-said materials creation technology, to develop materials and their application with attention paid to some specified functions. Under subject 3), self-complete type ceramic materials not requiring an external driving power source are to be developed, in which a selective cleaning function and environmental energy conversion function are amalgamated. (NEDO)

Fiscal 2000 achievement report. Welfare technosystem research and development (Chofu); 2000 nendo welfare technosystem kenkyu kaihatsu (Chofu) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

Research and development efforts are exerted to realize (1) a variable function residential circumstance system for the aged people and (2) a medical welfare and life assisting information system for helping the aged or handicapped people lead an independent life. Under item (1), a variable function residential circumstance system under which functions of the residence may be changed dependent upon inhabitants' degree of debility will be developed, and an information system will be developed under which medical welfare assistance and life assistance are integrated. Under item (2), residences, welfare appliances, and nurse/attendant assisting systems will be assessed and examined. In fiscal 2000, under item (1), a variable function residential unit was fabricated and was inhabited experimentally by people approximately 1 to 4 in the degree of need for nursing care, and problems were isolated to be discussed toward putting the system to practical use. Under item (2), physical power required for nursing and attending was measured using medical assistance appliances for in-home nursing care installed at Welfare Technohouse Chofu, and ergonomic studies were conducted with a view to relieving the load to be imposed upon those engaged in the nursing and attending work. (NEDO)

FY 1981 Report on the results of Sunshine Project. Coal energy; 1981 nendo sunshine keikaku seika hokokusho. Sekitan energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1981-07-01

This report presents the results of (researches on solvolysis liquefaction mechanisms and reaction promotion with oil- and coal-based solvents), conducted as part of the research and development project for coal liquefaction techniques. The FY 1981 program includes researches on (1) the effects of liquefaction reaction conditions on liquefaction yield and production of light products for coal species of low degree of carbonization, including brown coal, (2) the effects of pretreatment of coal on its liquefaction reactivity, and (3) up-grading of the solvolysis coal liquid (SCL). For the item (1), HA240 (hydrogenated Ashland's A240) is used to investigate its liquefaction capacity for various coal species of low degree of carbonization, including brown coal. For the item (2), the effects of pretreatment in a hot water bath with reflux was investigated for sub-bituminous coal in the FY 1980. In the FY 1981, various pretreatment methods are attempted for enhancing liquefaction reactivity of brown coal. As a result, it is found that ash content of brown coal is decreased to one-third of the initial level, when it is treated in a diluted hydrochloric acid bath with reflux. For the item (3), SCL hydrogenated by Birch reduction (B-SCL, 1) is compared with H-SCL with respect to properties, structures and thermal crackability, to discuss the items required for the catalyst to be used in the second stage by clarifying effectiveness of the catalyst. (NEDO)

Summary of the FY 1988 Sunshine Project results. Hydrogen energy; 1988 nendo sunshine keikaku seika hokokusho gaiyoshu. Suiso energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1989-04-01

Outlined herein are the results of researches on hydrogen energy as part of the FY 1988 Sunshine Project results. Researches on the techniques for producing hydrogen by electrolysis of water using a polymer electrolyte include development of power-supplying materials for electrolysis at high current density, and basic studies on the electrolysis using an OH ion conducting type polymer electrolyte. Researches on the techniques for producing hydrogen by electrolysis with hot steam include development of the materials, techniques for processing these materials, and electrolysis performance tests. Researches on the techniques for transporting hydrogen by metal hydrides include development of hydrogen-occluding alloys of high bulk density, and techniques for evaluating characteristics of metal hydrides. Researches on the techniques for storing hydrogen include those on alloy molding/processing techniques, hydrogen-storing metallic materials, and new hydrogen-storing materials. Researches on the techniques for utilizing hydrogen include those on energy conversion techniques using hydrogen-occluding alloys, and hydrogen-fueled motors. Researches on the techniques for safety-related measures include those on prevention of embrittlement of the system materials by hydrogen. (NEDO)

Summary of the FY 1989 Sunshine Project results. Hydrogen energy; 1989 nendo sunshine keikaku seika hokokusho gaiyoshu. Suiso energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1990-04-01

Outlined herein are the results of researches on hydrogen energy as part of the FY 1989 Sunshine Project results. Researches on the techniques for producing hydrogen by electrolysis of water using a polymer electrolyte include those on the SPE electrolysis at high temperature and current density, and basic studies on the electrolysis using an OH ion conducting type polymer electrolyte. Researches on the techniques for producing hydrogen by electrolysis with hot steam include development of the materials, techniques for processing these materials, and electrolysis performance tests. Researches on the techniques for transporting hydrogen by metal hydrides include development of hydrogen-occluding alloys of high bulk density, and techniques for evaluating characteristics of metal hydrides. Researches on the techniques for storing hydrogen include those on hydrogen-storing metallic materials, alloy molding/processing techniques, and new hydrogen-storing materials. Researches on the techniques for utilizing hydrogen include those on energy conversion techniques using hydrogen-occluding alloys, and hydrogen-fueled motors. Researches on the techniques for safety-related measures include those on prevention of embrittlement of the system materials by hydrogen. (NEDO)

Fiscal 2000 achievement report. Welfare technosystem research and development (Kamogawa); 2000 nendo welfare technosystem kenkyu kaihatsu (Kamogawa) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

For the purpose of assisting in-home medical care, a system to enable in-home acquisition, compression, accumulation, distribution, and regeneration of auscultatory sounds (cardiac sound, respiratory sound) is added to telemedicine assisting system technologies for remotely located doctors' decisions and instructions for in-home medical care. In fiscal 2000, for improvement on the already-developed system, operationality was improved, uptake was realized of still images (digital camera pictures) mainly for diseases in dematology where such is strongly demanded, and a transmitter function was augmented. The system enables the PHS (personal handyphone system) aided exchange of medical data between portable terminals on patrol and (doctors') personal computers through the personal computer server in the hospital. Doctors are able to administer remote telemedicine now that biomimetic information on remotely located patients such as still medical images and auscultatory sounds necessary for diagnosing is available in the hospital. (NEDO)

The effect of MRN complex and ATM kinase inhibitors on Zebrafish embryonic development

Science.gov (United States)

Kumaran, Malina; Fazry, Shazrul

2018-04-01

Zebrafish is an ideal animal model to study developmental biology due to its transparent embryos and rapid development stages of embryogenesis. Here we investigate the role of DNA damage proteins, specifically Mre11/Rad50/NBN (MRN) complex and ataxia-telangiectasia mutated (ATM) kinase during embryogenesis by inhibiting its function using specific MRN complex (Mirin) and ATM Kinase inhibitors (Ku60019 and Ku55933). Zebrafish embryos at midblastula transition (MBT) stage are treated with Mirin, Ku60019 and Ku55933. The embryonic development of the embryos was monitored at 24 hours-post fertilisation (hpf), 48 hpf and 72 hpf. We observed that at the lowest concentrations (3 µM of Mirin, 1.5 nM of Ku60019 and 3 nM of Ku55933), the inhibitors treated embryos have 100% survivability. However, with increasing inhibitor concentration, the survivability drops. Control or mock treatment of all embryos shows 100 % survivability rate. This study suggests that DNA damage repair proteins may be crucial for normal zebrafish embryo development and survival.

Lastedisain ehk kodumaine disainerlooming lastele / Dagmar Reinolt

Index Scriptorium Estoniae

Reinolt, Dagmar

2015-01-01

Eesti disainerite poolt toodetud lastepärased esemed - võileivaalus (VarmCountry), tapeet "Värvi ise" (Anni Varm), söögitool "Mari" (Julia Maria Künnap), nööpsadul (Liisa Elken), öökullipadi (Ann Lutter), kiikhobu "Fox" (Maile Grünberg), KuKuJuKu helkurid (Okeiko) ja Dadamora lasteriided (Mariliis Oksaar,Tõnis Kenkmaa)

Structural Modification of Egyptian Kaolinite for Paper Coating

International Nuclear Information System (INIS)

Mohamed, H.F.; Hassan, M.S.; Morsy, F.A.; El-Sherbiny, S.

2013-01-01

The aim of this work is surface modification of Egyptian kaolinite for paper coating. Kaolinite < 2 fraction (KF) obtained using sedimentation and centrifuging techniques. Organo-kaolinite was prepared via inserting Urea (NH 2 CO NH 2 ) within the gallery of kaolinite using grinding technique for different times. Nano kaolinite was prepared from dispersion of organo-kaolinite in paper coating. The kaolinite (K), kaolinite /urea (KU) and kaolinite urea with binder (KU/Binder) were characterized using X-ray diffractometry (XRD), Infrared spectroscopy (IR) and Scanning Electron Microscopy (SEM). XRD studies revealed shifting of basal space of kaolinite from 0.714 to 1.11 nm upon grinding of kaolinite with urea for 5 h. On the other hand, the characteristic peak of kaolinite completely disappeared during dispersion of KU in paper coating suspension, which revealed the exfoliation of organo-kaolinite layers through the binder. Meanwhile, IR spectra show that NH-CO molecule exists in the intercalated kaolinite. The SEM images of KU revealed that kaolinite is intercalated U and fully exfoliation of KU achieved in KU/Binder and revealed by thin flakes with particle size ranged from 500 nm and 300 nm respectively. The modified kaolinite for paper coating increased the optical properties in terms of ISO brightness, opacity and gloss of the coated paper. There was a significant decrease in coated paper roughness compared to untreated kaolinite. Air permeance of the KF decreased in comparison with K sample, but increased sharply by intercalation of kaolinite (KU). Burst strength also started to increase with untreated kaolinite but decreased sharply with urea KU.

Safety Evaluation Report related to the renewal of the operating license for the research reactor at the University of Kansas (Docket No. 50-148)

International Nuclear Information System (INIS)

1984-05-01

This Safety Evaluation Report for the application filed by the University of Kansas (KU) for a renewal of Operating License R-78 to continue to operate the KU 250-kW open-pool training reactor has been prepared by the Office of Nuclear Reactor Regulation of the US Nuclear Regulatory Commission. The facility is owned and operated by the University of Kansas and is located on the KU campus in Lawrence, Douglas County, Kansas. The staff concludes that the reactor facility can continue to be operated by KU without endangering the health and safety of the public. 17 references, 11 figures, 4 tables

Lie. kū́nas

Directory of Open Access Journals (Sweden)

Simas Karaliūnas

2011-12-01

Full Text Available LIТН. kū́nas “BODY”SummaryThe cognates of Lith. kū́nas “body” and Latv. kûnis (kи̃пе, kũņа “body; chrysalis; caterpil­lar of a butterfly; bee pupae” are supposed to be Lith. kūпа “carrion”, pa-kū́nė “sore, furuncle; upper lamella, a layer under the roots”, Latv. kипа “wart, excrescence”, kunis “bottom of a sheaf” and others. Lith. kū́nas, kūпа may represent substantivized forms of the adjective Latv. kûns“round, obese, stout”, while Latv. kûnis, kũņа, kūne seem to be derivatives of the suffixes *-o-*-ā-, *-ē-.

Erection of the Meiko-Central bridge; Meiko chuo ohashi no kasetsu

Energy Technology Data Exchange (ETDEWEB)

Kaneda, S.; Nishikawa, K.; Nagai, Y.; Ishihara, S.; Tsunekawa, M.; Yoshino, S. [Kawasaki Heavy Industries, Ltd., Kobe (Japan)

1997-10-20

This is a 1170m-long triple-span continuous steel cable-stayed bridge with the central span measuring 590m, built to traverse Nagoya Port as part of the Isewangan highway. The 190m-high main towers are A-shaped, and have an octagonal cross section to stay stable against winds. The joints were designed to be welded mainly on the spot so as not to affect the surrounding landscape, and in this welding process the achievement of prescribed accuracy was the important technological task. The main girder was a 37m-wide multi-chamber/single-box type, and the steel stabs were installed by on-site welding. Since the erection was carried out above the ship course, a method was used in which the main girder was hung over with the cable stretched long, which demanded constant prudence to assure accuracy throughout the work. The cable is a 17-stage 2-face multi-fan type, a semi-parallel wire strand coated with polyethylene and white fluoroethylene resin. As for accuracy management for erection, it was accomplished by adjusting the amount of shim introduced into each of the four cables every time one stage of cable was erected for the side span and central span. Every accuracy management requirement met the target value with an adequate margin. 6 refs., 10 figs., 4 tabs.

Repair of DNA DSB in higher eukaryotes

International Nuclear Information System (INIS)

Wang, H.; Perrault, A.R.; Takeda, Y.; Iliakis, G.

2003-01-01

Cells of higher eukaryotes process within minutes double strand breaks (DSBs) in their genome using a NHEJ apparatus that engages DNA-PKcs, Ku, DNA ligase IV, XRCC4, and other as of yet unidentified factors. Although chemical inhibition, or mutation, in any of these factors delays processing, cells ultimately remove the majority of DNA DSBs using an alternative pathway operating with slower kinetics. This alternative pathway is active in mutants deficient in genes of the RAD52 epistasis group. We proposed, therefore, that it reflects an alternative form of NHEJ that operates as a backup (B-NHEJ) to the DNA-PK- dependent (D-NHEJ) pathway, rather than homology directed repair of DSBs. We studied the role of Ku and DNA-PKcs in the coordination of these pathways using as a model end joining of restriction endonuclease linearized plasmid DNA in whole cell extracts. Efficient error-free endjoining observed in such in-vitro reactions is strongly inhibited by anti-Ku antibodies. The inhibition requires DNA-PKcs, despite that fact that Ku efficiently binds DNA ends in the presence of antibodies, or in the absence of DNA-PKcs. Strong inhibition of DNA endjoining is also mediated by wortmannin, an inhibitor of DNA-PKcs, in the presence but not in the absence of Ku, and this inhibition can be rescued by pre-incubating the reaction with double stranded oligonucleotides. The results are compatible with a role of Ku in directing endjoining to a DNA-PK dependent pathway, mediated by efficient end binding and productive interactions with DNA-PKcs. On the other hand, efficient end joining is observed in extracts of cells lacking DNA-PKcs, as well as in Ku-depleted extracts sugggesting the operation of alternative pathways. Extracts depleted of Ku and DNA-PKcs rejoin blunt ends, as well as homologous ends with 3' or 5' protruding single strands with similar efficiency, but addition of Ku suppresses joining of blunt ends and homologous ends with 3' overhangs. We propose that the

Volcanology of Tuzo pipe (Gahcho Kué cluster) — Root-diatreme processes re-interpreted

Science.gov (United States)

Seghedi, I.; Maicher, D.; Kurszlaukis, S.

2009-11-01

The Middle Cambrian (~ 540 Ma) Gahcho Kué Kimberlite Field is situated about 275 km ENE of Yellowknife, NWT, Canada. The kimberlites were emplaced into 2.6 Ga Archean granitic rocks of the Yellowknife Supergroup. Four larger kimberlite bodies (5034, Tesla, Tuzo, and Hearne) as well as a number of smaller pipes and associated sheets occur in the field. In plan view, the Tuzo pipe has a circular outline at the surface, and it widens towards deeper levels. The pipe infill consists of several types of coherent and fragmental kimberlite facies. Coherent or apparent coherent (possibly welded) kimberlite facies dominate at depth, but also occur at shallow levels, as dikes intruded late in the eruptive sequence or individual coherent kimberlite clasts. The central and shallower portions of the pipe consist of several fragmental kimberlite varieties that are texturally classified as Tuffisitic Kimberlites. The definition, geometry and extent of the geological units are complex and zones controlled by vertical elements are most significant. The fluidal outlines of some of the coherent kimberlite clasts suggest that at least some are the product of disruption of magma that was in a semi-plastic state or even of welded material. Ragged clasts at low levels are inferred to form part of a complex peperite-like system that intrudes the base of the root zone. A variable, often high abundance of local wall-rock xenoliths between and within the kimberlite phases is observed, varying in size from sub-millimeter to several tens of meters. Wall-rock fragments are common at all locations within the pipe but are especially frequent in a domain with a belt-like geometry between 120 and 200 m depth in the pipe. Steeply outward-dipping bedded deposits made up of wall-rock fragments occur in deep levels of the pipe and are especially common under the downward-widening roof segments. The gradational contact relationships of these deposits with the surrounding kimberlite-bearing rocks as well

Beyond repair foci: DNA double-strand break repair in euchromatic and heterochromatic compartments analyzed by transmission electron microscopy.

Directory of Open Access Journals (Sweden)

Yvonne Lorat

Full Text Available DNA double-strand breaks (DSBs generated by ionizing radiation pose a serious threat to the preservation of genetic and epigenetic information. The known importance of local chromatin configuration in DSB repair raises the question of whether breaks in different chromatin environments are recognized and repaired by the same repair machinery and with similar efficiency. An essential step in DSB processing by non-homologous end joining is the high-affinity binding of Ku70-Ku80 and DNA-PKcs to double-stranded DNA ends that holds the ends in physical proximity for subsequent repair.Using transmission electron microscopy to localize gold-labeled pKu70 and pDNA-PKcs within nuclear ultrastructure, we monitored the formation and repair of actual DSBs within euchromatin (electron-lucent and heterochromatin (electron-dense in cortical neurons of irradiated mouse brain.While DNA lesions in euchromatin (characterized by two pKu70-gold beads, reflecting the Ku70-Ku80 heterodimer are promptly sensed and rejoined, DNA packaging in heterochromatin appears to retard DSB processing, due to the time needed to unravel higher-order chromatin structures. Complex pKu70-clusters formed in heterochromatin (consisting of 4 or ≥ 6 gold beads may represent multiple breaks in close proximity caused by ionizing radiation of highly-compacted DNA. All pKu70-clusters disappeared within 72 hours post-irradiation, indicating efficient DSB rejoining. However, persistent 53BP1 clusters in heterochromatin (comprising ≥ 10 gold beads, occasionally co-localizing with γH2AX, but not pKu70 or pDNA-PKcs, may reflect incomplete or incorrect restoration of chromatin structure rather than persistently unrepaired DNA damage.Higher-order organization of chromatin determines the accessibility of DNA lesions to repair complexes, defining how readily DSBs are detected and processed. DNA lesions in heterochromatin appear to be more complex, with multiple breaks in spatial vicinity inducing

Näitus nagu kuu / Anders Härm

Index Scriptorium Estoniae

Härm, Anders, 1977-

2005-01-01

Näitusest "Expat-Art-Centre" Tallinna Kunstihoone fuajees, Ku fassaadil ja Ku-Ku kohvikus kuni 12. I. Näituse ja sellega kaasneva ajakirja on koostanud Mathieu Copeland, osalevad Brian Eno, Pierre Huyghe (sünd. 1962), Ben Kinmont, Claude Leveque (sünd. 1953), Didier Marcel, Olivier Mosset (sünd. 1933), Shimabuku (sünd. 1969), Dan Walsh (sünd. 1962), Ian Wilson (sünd. 1940)

Natural course of sensitization to hen's egg in children not previously exposed to egg ingestion.

Science.gov (United States)

de Boissieu, D; Dupont, C

2006-04-01

Clinical adverse reactions to egg may occur in infants or children who have never eaten egg. They may be sensitized or even react at first egg ingestion. Few studies are available concerning the reality of egg white allergy in such sensitized children, the natural evolution of this condition and the appropriate decisions to make. To analyze the actuality and natural course of egg allergy in children sensitized without previous of hen's egg ingestion. We set up a clinical decision tree based on clinical history and specific egg white IgE to manage patients who had never ingested egg but were sensitized as demonstrated by a positive SPT and report a cohort of 30 such children The mean level of egg white specific IgE at first analysis, i.e. before 12 months, was high, 28.3 KU(A) /L, with a large range, from 0.6 to >100 KU(A) /L, below 6 KU(A) /L in only 8 patients. In 6 children ("no challenge" group), IgE values remained >8 KU(A) /L by the end of the survey and the oral challenge with egg was always denied. Their mean + SD IgE level was at 51.7 + 38 KU(A) /L at 1 year and 19.7 + 13 KU(A) /L at a mean age of 34 + 5 months. All had an associated anaphylactic reaction with milk and 5 were still allergic to milk by the end of the survey. In the remaining 24 infants, egg was given for the first time at a mean age of 30 + 9 months, by error in 4 cases, all exhibiting an immediate reaction, and in a hospital setting in 20, among whom 14 reacted. Among those 18, with a specific IgE level at 9.1 + 10 KU(A) /L at 28 + 9 months, 4 became tolerant between 3 and 4 years, with specific IgE levels below 1.3 KU(A) /L and a 5th one with specific IgE >100 KU(A) /L at 6 months tolerated scrambled eggs at age 7 year, with specific IgE at 2.6 KU(A) /L. In the 6 others, labeled "non allergic", egg white specific IgE levels were significantly lower, whatever the age, than in the "no challenge" group. The age at challenge was 35 + 8 months, with a mean specific IgE level at 1.0 + 0.9 KU

Validitas dan Reliabilitas Konstruk Pengukuran Perpustakaan Ideal Berbasis Pemakai dengan Pendekatan LIBQUAL

Directory of Open Access Journals (Sweden)

Yulyanti Fahruna

2017-08-01

Full Text Available This study aims to measure the validity and reliability construct satisfaction and quality of library services based on the user using LIBQUAL (library quality method with a touch of marketing management. Data analysis by Cronbach Alpha and Pearson Correlation. The research was conducted in Tanjungpura University (UNTAN by taking samples of visitors (students at six (6 faculty libraries namely three (3 social faculty of 150 people and three (3 science faculty of 150 people. The sample is chosen by purposive sampling method. Reliability test results show that the five US, IC, LP, LQ and KU variables show high instrument reliability. The result of pearson correlation test shows that the measurement instrument used has problem validity on some question items. At minimum service item with correlation value smaller than 0.30 is IC2, IC5 and LQ5 so it is declared invalid. At service expectation items with correlation value smaller than 0.30 ie AS8, IC1, IC2, IC4, LP1, KU1, KU2, KU4, and KU8. And at present service items with correlation values smaller than 0.30 ie IC1, IC3, LP1, LP3, LQ1, LQ3, LQ4 and KU1.

Siim Nestor soovitab : Odessa Pop. Neoandertals / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2007-01-01

Indieüritusest Odessa Pop 17. veebr. Tallinnas baar-klubis Juuksur. Ultrabrutaalse death-metali duo Neoandertals heliplaadi "Neoandertals Were Master Butchers" esitluskontserdist 17. veebr. Tallinnas KuKu klubis

1998 Annual Study Report. Standardization of color reproduction management; 1998 nendo seika hokokusho. Iro saigen kanri (color management) no hyojunka

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

Standardization of color reproduction management is aimed at realization of the most urgently needed areas of the color reproduction management for international standardization. This R and D program is aimed at drafting the standards for color information devices and their characteristics, and proposing the drafts. Factors which deteriorate color reproduction stability of input and output devices are classified and analyzed, devices for evaluation are selected, and extents of the deterioration are measured and evaluated, in order to extract the problems involved in the color reproduction models for the current input and output devices. Standardization of color management on the multi-spectrum color images is attempted for business printing, where effectiveness of a fluorescent ink containing a fluorescent material is noted for expansion of the color reproduction range for printing color images with the conventional YMCK inks. Basic aspects of the fluorescent colors are measured, to verify the methods for measuring fluorescent body colors. As a result, new knowledge is obtained. The standard images for flesh color evaluation are produced, and the methods for analyzing the data obtained by the tests, including the subjective tests, are established. These lead to establishment of the standards for preferred flesh color of the yellow races. (NEDO)

FY 1998 R and D project on industrial science technology; 1998 nendo ryoshi kino soshi no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

The R and D on quantum functional devices (QFDs) were conducted, and the FY 1998 results were summed up. In the comprehensive survey on QFDs, the following were carried out for the efficient R and D promotion: R and D progress state survey/problem extraction/analytical study, technical trend survey, overseas survey ordered from overseas supplier, common basement technology study, etc. In the R and D on technology for QFDs, integration of multivalued logical devices using tunneling control functional devices, integration of logical memory devices using quantum levels, integration of quantum band combination-type multi-functional devices, silicon insulating film tunnel memory devices, assembly quantum dot functional memory, quantum wave switching functional devices, integration of single electron logical devices, integration of CMOS combination-type single electron devices, etc. Moreover, in the development of the basement technology of single electron devices, technology to construct element devices using quantum functions, basement technology of single electron device integration devices, architecture of single electron device information processing circuit system, etc. (NEDO)

Summary of achievement reports on the Sunshine Project in fiscal 1978 (Hydrogen energy); 1978 nendo seika hokokusho gaiyoshu. Suiso energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1979-04-01

This paper summarizes achievement reports on the Sunshine Project in fiscal 1978 (hydrogen energy). In hydrogen manufacturing methods, studies are described on materials of membranes and electrodes used in high temperature and pressure electrolysis. In thermo-chemical method, studies are continuing on cycles of the iron system, iodine system, and mixed system (composed by thermal, photo and electro-chemistries). For the iodine system, summary design was performed on an experimental device. For the mixed system, trial fabrication and experiments were carried out on a beam radiation type electrolytic tank that electrolyses quickly HI and Fe{sup 3+} produced in the photo-chemical reaction, and separates the products. Discussions were also given on HI decomposition (hydrogen acquisition) by means of heat diffusion. With respect to storage and transportation, development is being made on optimal metal hydrides. In combustion technologies, discussions are given on combustors and catalysts to break through the dilemma of high NOx emission and frequent occurrence of reverse ignition. For fuel cells, the paper describes developments of the materials thereof, high-temperature solid electrolyte type fuel cells and alkaline aqueous solution electrolyte type fuel cells. Regarding the non-steady hydrogen engines, the paper describes fundamental studies on non-steady jet flow behavior using shock tubes, and single cylinder engine tests. It also describes hydrogen safety assuring measures, and studies on energy systems. (NEDO)

Achievement report for fiscal 1998. Home rehabilitation system for aphasia patients; 1998 nendo seika hokokusho. Shitsugosho zaitaku rehabilitation shien system

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-05-01

Development has been made on the element technologies for a home rehabilitation system for aphasia patients. First, studies were performed on an authoring procedure for aphasia patient training, and partial trial production and development were carried out on an authoring system for the training program formula. Then, studies were executed on a training simulation and analysis filter, and partial trial production and development were implemented on a home rehabilitation supporting and self-teaching system. Next, an input and output mechanism for voice signal processing was studied, and aphasia patient and caregiver human interface was partially fabricated on a trial basis. In addition, a remote rehabilitation communicating method was researched, and partial trial production was carried out on a remote rehabilitation evaluation and diagnosis support means. Finally, communication assisting means were researched, and a communication assisting system was partially produced on a trial basis. Good evaluation results were given on a demonstration system. Although partial indications were given on minute parts, practical agreement was obtained as a whole. Great expectation was given particularly on the remote rehabilitation system and the authoring system. (NEDO)

Geothermal development promotion survey. No. B-3 Kumaishi area; Chinethu kaihatsu sokushin chosa seika hokokusho. No. B-3. Kumaishi chiiki

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

This paper summarizes the result of a geothermal resource survey carried out from fiscal 1995 through fiscal 1997 in the Kumaishi Area located in the western center of Oshima Peninsula in the south western part of Hokkaido. The survey for this area was performed with an objective to search the spread of high temperature area continuing from the adjoining Yakumo area where the highest temperature of 233 degrees C has been confirmed in a structural test well (N63-YK-6), to identify high water permeating areas in great depth points underground, and to find possibility of existence of geothermal resources. The survey included geological and alteration zone survey, geochemical survey, gravitation exploration, electromagnetic exploration, drilling and temperature logging of five structural test wells, water injection test, temperature restoration test, core survey and hot water survey. Also performed were environmental effect survey, rock age measurement, alteration age measurement, and fluid inclusion test. As a result of the survey, it was identified that the verified geothermal hot water has been stored in granites with poor water permeability, whose low temperature has not formed convection systems spreading in wide areas despite having been heated, making each system exist in small scale independently. It was estimated that the possibility is low to expect geothermal power generation. (NEDO)

Development of environmentally friendly universal controller. Data and materials; 1998 nendo kankyo taio universal controller no kaihatsu seika hokokusho. Shiryohen

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-05-01

Research and development was carried out for an 'environmentally friendly universal controller (UC)' which would be 'user-friendly and energy-saving' when used with electrical home appliances, and data and materials on them were compiled into this volume. It covers the specifications (Versions 0.6 and 0.51) of a newly developed two-way controller protocol, the specification (Version 0.1) of two-way remote control IrDA-C communication unit hardware, an infrared communication protocol IrDa Control, and the like. It also accommodates information collected through surveys of technological trends as reflected in technical literature, Internet home pages, catalogs, and the like, concerning the user-friendliness of electrical home appliances, remote control, domestic consumption of electric power, and the like. Also covered is the result of surveys of patents involving environmentally friendly UC related technologies, namely, Japanese patents (from applications made in 1979 through disclosures made in 1998) and U.S. patents (registrations made in 1982 and thereafter). Furthermore, it contains the result of surveys of actualities of domestic use conducted for the purpose of clarifying the effect of environmentally friendly UC introduction, how such UCs are being accepted by the public, and the conditions that should be satisfied for their popularization. (NEDO)

Report on results of contract research. 'Research on MHD generation system'; MHD hatsuden system no kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1982-03-01

'Research on MHD generation system' was implemented by its expert committee in the electric joint study group, with the results of fiscal 1981 reported. This year, technological reexamination was conducted for a 2,000 MWt commercial MHD generation plant, with evaluation carried out on the cost performance including the construction and operation cost. In addition, for the purpose of intermediate R and D towards the practicability, examination was also conducted on a system structure, concrete specifications of component element, cost of R and D including operation expenses for example, concerning an 100 MWt class experimental plant and a 500 MWt class plant. In the investigation of the overseas trend, information was summarized in detail on the experimental devices, combustors, generation channels, electrode materials, electrode phenomena, theoretical analyses, seeds, slag, component equipment, instrumental technologies, conceptual designs of generation plant, commercial plant, etc., in Soviet Union, China, Holland, India and EPRI, on the basis of the materials from the 19th MHD symposium held in UTSI and from the coal MHD specialist conference held in Sydney. (NEDO)

Fiscal 1998 achievement report on welfare technosystem research and development. Kyoto; 1998 nendo walfare technosystem kenkyu kaihatsu (Kyoto) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

To be ready for the computing-everywhere age to come, it is necessary to create environments in which the barrier-free utilization is ensured of various apparatuses of the daily routine, such as various bodily function substituting apparatuses capable of compensating for the degraded functions of disabled or aged individuals. Under the circumstances, a system for building man-machine interfaces in the home or the like is required, and fundamental technologies of architecture and information infrastructure have to be established on which the development of technologies for the field involved will proceed. Concerning the technologies already in existence in this field of research, a survey is conducted of the status of research and development of information interface techniques primarily at Stanford University, and a report is made thereon. Also reported is the information obtained at Technology and Persons with Disabilities Conference 1999. The result of a survey of the trend of research and development of the smart house under the TIDE (Technology Initiative for Disabled and Elderly People) project and the result of a survey of an information standardization project for equipment control in Europe are reported, and the result of a survey of the approach of Kyoto's welfare apparatus distributors to the development of equipment is made known. (NEDO)

Summary of reports on 1979 result of Sunshine Project. Solar energy; 1979 nendo sunshine keikaku seika hokokusho gaiyoshu. Taiyo energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1980-04-01

This report is a compilation of all outlines of the results concerning 'solar energy' for which R and D was carried out as a part of Sunshine Project in fiscal 1979. The research subjects (items of the studies) are written below. 1. Solar energy system (measurement of spectral irradiance, utilization system, and meteorological investigation); 2. Solar thermal power generation system; 3. Photovoltaic power generation system (basic research on solar cells, silicon vertical ribbon crystal, silicon horizontal ribbon crystal, particle non-acceleration growth type thin film silicon crystal, particle acceleration growth type thin film silicon crystal, new type solar cells, secondary to quaternary compound semiconductor solar cells, and photovoltaic power generation system); 4. Solar cooling, heating and hot water supply system (evaluation system, newly-built private residential system, existing private residential system, multiple dwelling system, large building system, synthetic resin materials, glass based materials, and metallic materials); 5. Solar energy new utilization method (new power generation system and materials); 6. R and D on solar thermal power generation plant (R and D on pilot plant, experimental research for developing plant on curved surface converging method, and experimental research for developing plant on tower converging method). (NEDO)

Report on results of contract research. 'Research on MHD generation system'; MHD hatsuden system no kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1981-03-01

'Research on MHD generation system' was implemented by its expert committee in the electric joint study group, with the results of fiscal 1980 reported. This year, a detailed conceptual design was carried out on a coal fired MHD generation system, with points for the technological development concretely examined. In addition, investigation was conducted on the progress of MHD generation technology, development situation of other generation systems, state of energy resources, etc., in various foreign countries. In the conceptual design of the coal fired MHD generation plant, the system structure of a 2,000 MWt class commercial MHD generation plant was explained, as were the conceptual design of the structural elements and proposals for a 500 MWt class demonstration plant and an 100 MWt class experimental plant, for example. In the overseas trend of R and D on MHD generation, investigations were made concerning the U.S., Soviet Union, and China, with details compiled for such items as generation plants, combustors, generation channels, heat resisting materials, superconducting magnets, heat exchangers, seed slags, inverters, boilers and environments, and commercial plants. (NEDO)

Fiscal 1999 achievement report. Development of ultralow-loss power device technology; 1999 nendo choteisonshitsu denryoku soshi gijutsu kaihtsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

The aim is to establish basic technologies for upgrading wide-gap semiconductor devices, fabricated mainly of SiC, in terms of their low-loss, high-speed, and high-power features. In the research and development of ultralow-loss power device technology, progress of the effort is reviewed, problems in the process of research and development are extracted, and technological trends are surveyed. In the development of basic technologies, an SiC crystal growing device is experimentally built and the process of crystal growth is assessed and analyzed, and tasks to discharge for higher quality and larger diameter are extracted. Basic technologies are developed relative to the epitaxial growth, interface control, and conductivity control of SiC etc. In the development of technologies for developing SiC into devices, technological development is carried out for the junction-type FET (field effect transistor), which involves termination structure optimization, high-voltage capability enhancement, and gate-off gain improvement. As for MOSFET (metal oxide semiconductor FET), MOS channel formation technology is developed and device-constructing technology is also developed. As for MESFET (metal-semiconductor FET), micro-processing is established for a success in experimentally building a 0.5{mu}m-long gate. (NEDO)

Fiscal 1998 achievement report on welfare technosystem research and development. Tokushima; 1998 nendo walfare technosystem kenkyu kaihatsu (Tokushima) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

The project aims to construct a real-time system capable of searching a wider living area through establishing a management system centralized by remote control by improving on radio transmitters in a communication network and on a central station communication control system centering about a health and welfare office, for alleviating the burden of the nursing of old persons who wander, for preventing them from accidents attributable to wandering, and for finding and protecting them in time. For the construction of such a system, surveys and studies are conducted for the preparation of fundamental designs for personal data files on individuals carrying radio transmitters, monitor screen manipulation, program for acquiring information from a transmitter about its current position and for receiving information from the terminal station, and a frequency selecting program or the like to give instructions to the terminal station to probe the frequency on which the missing person's transmitter operates. In this fiscal year, specifications are studied of software programs based on data obtained from an experiment conducted for the practical application of a search network system utilizing the currently available radio direction finding technique, which effort is exerted mainly at the infirmary of Hanoura-cho, Tokushima Prefecture. (NEDO)

Development of environmentally friendly universal controller. Data and materials; 1998 nendo kankyo taio universal controller no kaihatsu seika hokokusho. Shiryohen

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-05-01

Research and development was carried out for an 'environmentally friendly universal controller (UC)' which would be 'user-friendly and energy-saving' when used with electrical home appliances, and data and materials on them were compiled into this volume. It covers the specifications (Versions 0.6 and 0.51) of a newly developed two-way controller protocol, the specification (Version 0.1) of two-way remote control IrDA-C communication unit hardware, an infrared communication protocol IrDa Control, and the like. It also accommodates information collected through surveys of technological trends as reflected in technical literature, Internet home pages, catalogs, and the like, concerning the user-friendliness of electrical home appliances, remote control, domestic consumption of electric power, and the like. Also covered is the result of surveys of patents involving environmentally friendly UC related technologies, namely, Japanese patents (from applications made in 1979 through disclosures made in 1998) and U.S. patents (registrations made in 1982 and thereafter). Furthermore, it contains the result of surveys of actualities of domestic use conducted for the purpose of clarifying the effect of environmentally friendly UC introduction, how such UCs are being accepted by the public, and the conditions that should be satisfied for their popularization. (NEDO)

Fiscal 1999 research achievement report on the development of SNPs related technologies; 1999 nendo SNPs kanren gijutsu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

Efforts are made to develop specimen processing technologies for modifying and enabling various kinds of specimens to automatically undergo SNP (single nucleotide polymorphism) analysis for medicine development and clinical diagnostic activities and to develop technologies and apparatuses to enable rapid, inexpensive, and simple search and analysis of SNPs using DNA (deoxyribonucleic acid) chips and mass spectrometry. Activities are conducted in the four fields involving (1) the development of a practical clinical system for rapid detection and analysis of SNPs, (2) research and development of an SNP scoring system using bar-coded oligonucleotides and magnetic beads, (3) research and development of a high-speed SNP analysis system using a mass spectrometer, and (4) the development of a high throughput SNP analysis line. Efforts exerted in field (1) involve a protein fixation method using plasma polymerization and its application to DNA arrays, development of an SNP detection method using human genomes, construction of a rapid DNA detection device using an electric field, development of an SNP analysis system using the solid phase HPA (hybridization protection assay) method, and SNP analysis using solid phase ligation. (NEDO)

ONR Far East Scientific Bulletin, Volume 12, Number 4, October-December 1987,

Science.gov (United States)

1987-12-01

magnetic Japan Control Engineers Measurements (CPEM󈨜) 1-35-28-303 Hongo , Bunkyo-ku, Tokyo 113 June The 7th International Kyoto, Professor Isao Yamada...Physics-Material Japan Faculty of Engineering, 0 Research Society) Inter- Tokyo University national Conference on 7-3-1 Hongo , Bunkyo-ku, Tokyo 113...o Department of Psychological 39-FlOO-J200 Chemistry and Nutrition, Faculty of Medicine, University of Tokyo 7-3-1 Hongo , Bunkyo-ku, Tokyo 113

Five-year outcomes of pars plana vitrectomy for macular edema associated with branch retinal vein occlusion

Directory of Open Access Journals (Sweden)

Nishida A

2017-02-01

Full Text Available Akihiro Nishida,1,2 Hiroshi Kojima,1,2 Takanori Kameda,1,2 Michiko Mandai,1,3 Yasuo Kurimoto1,2 1Department of Ophthalmology, Kobe City Medical Center General Hospital, 2Department of Ophthalmology, Institute of Biomedical Research and Innovation, 3Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Chuo-ku, Kobe, Hyogo, Japan Purpose: Long-term outcomes of pars plana vitrectomy (PPV for macular edema (ME associated with branch retinal vein occlusion (BRVO have been previously reported, but the studies did not report the number of additional treatments after surgery. During 5 years of follow-up, we therefore investigated the efficacy and safety of PPV for BRVO and evaluated the incidence of additional treatments. Methods: We retrospectively reviewed the medical records of 25 eyes of 24 patients who underwent PPV for ME associated with BRVO and were followed up for at least 5 years. Best-corrected visual acuity was measured, and foveal thickness was assessed by optical coherence tomography. Additional treatments were also investigated. Results: The logarithm of the minimal angle of resolution (logMAR improved from 0.53±0.23 at baseline to 0.16±0.25 at 5 years (P<0.0001. The foveal thickness decreased from 535±222 µm at baseline to 205±143 µm at 5 years (P<0.0001. For the eyes with residual ME, the following additional treatments were performed within 5 years of follow-up: sub-Tenon injection of triamcinolone acetonide in two eyes, intravitreal injection of bevacizumab in three eyes, grid laser photocoagulation in one eye, and direct photocoagulation of macroaneurysm in one eye. Additional surgeries were performed in two eyes: for one eye, phacoemulsification extraction of the ocular lens and intraocular lens implantation were performed because of cataract progression, and for the other eye, additional PPV was done for postoperative retinal detachment. Conclusion: PPV was effective for resolution of ME

Forodesine in the treatment of relapsed/refractory peripheral T-cell lymphoma: an evidence-based review

Directory of Open Access Journals (Sweden)

Makita S

2018-04-01

Full Text Available Shinichi Makita,1 Akiko Miyagi Maeshima,2 Dai Maruyama,1 Koji Izutsu,1 Kensei Tobinai1 1Department of Hematology, 2Department of Pathology, National Cancer Center Hospital, Chuo-ku, Tokyo, Japan Abstract: T-cell lymphoma is a rare hematologic malignancy with an incidence rate between 10% and 20% of that of non-Hodgkin lymphomas. Patients with peripheral T-cell lymphoma (PTCL generally have a poor prognosis when treated with cyclophosphamide, doxorubicin, vincristine, and prednisolone (CHOP/CHOP-like chemotherapy; once relapse occurs, it is mostly regarded as an incurable disease. To overcome the chemorefractoriness of PTCL, several novel agents have been developed. Since the first approval of pralatrexate, a dihydrofolate reductase inhibitor, for relapsed/refractory PTCL by the US Food and Drug Administration, several new agents, such as romidepsin (histone deacetylase inhibitor, brentuximab vedotin (antibody–drug conjugate targeting CD30, chidamide (histone deacetylase inhibitor, and mogamulizumab (anti-CC chemokine receptor 4 monoclonal antibody, have been approved as a therapeutic option for relapsed/refractory PTCL in several countries, including the US, Europe, China, and Japan. Forodesine is a novel, potent purine nucleoside phosphorylase inhibitor that is effective against T-cell malignancies. Although the clinical development of forodesine was discontinued in the US and Europe, a multicenter Phase I/II study of oral forodesine for relapsed PTCL was recently completed in Japan. The overall response rate was 24% (10 of 41 patients, which included four patients with complete response. In general, the toxicity of forodesine is manageable. As the study met the primary end point, forodesine was approved for the treatment of relapsed/refractory PTCL in Japan in March 2017, which was the first approval of forodesine in the world. As forodesine is an oral formulation, it is more convenient than other novel intravenous agents approved for PTCL

A novel method for calculating the energy cost of turning during running

OpenAIRE

Hatamoto, Yoichi; Yamada,Yosuke; Higaki,Yasuki; Kiyonaga,Akira; Tanaka,Hiroaki; Fujii,Tatsuya

2013-01-01

Yoichi Hatamoto,1 Yosuke Yamada,2 Tatsuya Fujii,3 Yasuki Higaki,3 Akira Kiyonaga,3 Hiroaki Tanaka31Graduate School of Sports and Health Science, Fukuoka University, Nanakuma Jonan-ku Fukuoka, Japan; 2The Fukuoka University Institute for Physical Activity, Nanakuma Jonan-ku Fukuoka, Japan; 3Faculty of Sports and Health Science, Fukuoka University, Nanakuma Jonan-ku Fukuoka, JapanAbstract: Although changes of direction are one of the essential locomotor patterns in ball sports, the physiologica...

Evaluation of mutagenic potential of contaminated atmosphere at Ibirapuera Park, Sao Paulo - SP, Brazil, using the Tradescantia stamen-hair assay

International Nuclear Information System (INIS)

Ferreira, Maria Izildinha; Domingos, Marisa; Gomes, Heliana de A; Saldiva, Paulo H.N.; Assuncao, Joao V. de

2007-01-01

Trad-SHM assay was used to check mutagenic potential of atmospheric contamination at Ibirapuera Park, located in Sao Paulo city, Brazil, and variation of risk along the year, besides determining which Tradescantia clone, BNL 4430 or KU-20, better indicates risk. Thirty pots of both clones were exposed during one-year period (September, 2002-August, 2003). Twenty inflorescences were taken from each clone twice a month in the morning, in order to estimate the frequency of mutations in stamen hairs. Results were compared to air pollution and climatic data measured next to the exposure site. KU-20 showed stamen-hair mutations greater than BNL 4430. Greatest mutation rates in KU-20 were observed in condition of high monthly mean of NO 2 and average peak concentrations of NO during the day, indicating that mutagenic effects originated from vehicular pollution. Clone KU-20 revealed to be more appropriate for biomonitoring purposes at the Park. - Clone KU-20 showed to be more appropriate than clone BNL 4430 to indicate vehicular pollution mutagenic risks in Trad-SHM assay

Kunstnike Liidu volikogu koosolek toimus 20. X Ku-Ku klubis

Index Scriptorium Estoniae

2003-01-01

Kinnitati EKLi 2004. a. eelarve, selle toetustest. Harry Liivrand tutvustas Kunstihoone ja selle galerii, Reet Varblane Linnagalerii, Elin Kard Hobusepea ja Draakoni galerii 2004. a. näituseplaane. Ateljee Vabaduse väljak 6-35 otsustati anda rendile Ülle Marksile, ateljee Ristiku 27 müüa Rafael Arutjunjanile. EKLi uued liikmed: Andrus Joonas, Alice Kask, Hermes Sarapuu, Epp Viires, Kristel Sibul

Somatic mutation frequencies in the stamen hairs of stable and mutable clones of Tradescantia after acute gamma-ray treatments with small doses

International Nuclear Information System (INIS)

Ichikawa, Sadao; Takahashi, C.S.

1977-01-01

Young inflorescences of two different Tradescantia clones heterozygous for flower and stamen-hair color, one stable (KU 9) and the other spontaneously mutable (KU 20), were irradiated acutely with small doses (approx. 3 to 50 R) of 60 Co gamma-rays. Somatic mutation frequencies from blue to pink in the stamen hairs scored on post-irradiation days 10 to 16 increased essentially linearly with increasing gamma-ray dose in both clones. Despite about a 5-fold difference in spontaneous mutation frequency per hair found between the two clones, the dose-response curves of pink mutations determined were similar to each other, giving average mutation frequencies of 1.51 and 1.41 pink-mutant events per 1000 hairs per R for KU 9 and KU 20, respectively. These frequencies are comparable to earlier results obtained from acute irradiation treatments of other clones with higher doses. The doubling dose of pink mutation (the radiation dose making the mutation frequency double the spontaneous level) was calculated to be 2.09 R for KU 9, and this low doubling dose must be given full attention. On the other hand, the doubling dose for KU 20 (calculated to be 10.4 R) is of questionable value, being greatly subject to change because of the diversely variable spontaneous mutation frequency of this clone

Endothelin-A-receptor antagonism with atrasentan exhibits limited activity on the KU-19-19 bladder cancer cell line in a mouse model.

Science.gov (United States)

Herrmann, Edwin; Tiemann, Arne; Eltze, Elke; Bolenz, Christian; Bremer, Christoph; Persigehl, Thorsten; Hertle, Lothar; Wülfing, Christian

2009-10-01

The endothelin axis consists of endothelin-1 (ET-1) and its two receptors, ET(A)- and ET(B)-receptor (ET(A)-R and ET(B)-R). In several tumor entities, the ET(A)-R plays a significant role as a drug target. In our study, we investigated whether inhibition of ET(A)-R with atrasentan leads to an antitumor effect in urinary bladder carcinoma as well. Twenty nude mice with thymic aplasia were subcutaneously administered 2 x 10(6) KU-19-19 bladder cancer cells in the right flank. Starting on the 22nd day after the injection, ten animals were treated with atrasentan (2.5 mg/kg BW intraperitoneally), and another ten animals were treated with placebo. During treatment, absolute tumor growth and relative growth rate over time were determined. After the end of treatment, the mitosis and necrosis rates, microvessel density, and receptor density in the tumor tissue were analyzed by immunohistochemistry. In addition, the expression intensities of ET-1, ET(A)-R, and ET(B)-R were evaluated semiquantitatively and compared between the groups. No significant differences between the active-treatment and placebo groups were detected, either with respect to absolute tumor growth (P = 0.333) or mitosis rate (P = 0.217). In the analysis of the necrosis rate and receptor density for ET(A)-R, a trend toward higher values in the active-treatment group (mean necrosis rate = 63.67%, receptor density: 1.417) than in the placebo group (mean necrosis rate = 46.25%, receptor density: 1.270) was found; however, neither difference was statistically significant (P = 0.08 and 0.219, respectively). ET(A)-R blockade with atrasentan in a bladder cancer xenograft model shows no significant antitumor effect.

Agresijas un vecāku audzināšanas stila saistība jauniešiem no profesionālās vidējās izglītības iestādēm

OpenAIRE

Stikāne, Austra

2012-01-01

Pētījuma jautājumi ir vai pastāv saistība starp agresijas rādītājiem un vecāku audzināšanas stilu profesionālās vidējās izglītības iestāžu audzēkņiem, kuri ikdienā dzīvo ar vecākiem un audzēkņiem, kuri ikdienā nedzīvo ģimenē, kā arī vai pastāv atšķirības agresijas skalās starp jauniešiem, kuri dzīvo ar vecākiem ikdienā un jauniešiem, kuri ikdienā nedzīvo ar vecākiem. Pētījumā piedalījās 95 respondenti (55 studenti, kuri ikdienā dzīvo ar vecākiem, 40 studenti, kuri ikdienā nedzīvo ģimenē)...

Report of the results of the fiscal 1997 survey. R and D of high efficiency clean energy vehicles; 1997 nendo chosa hokokusho. Kokoritsu clean energy jidosha no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

For the purpose of developing an automobile which keeps low pollution using petroleum substituting clean energy, decreases the running energy consumption to a half at least, and reduces the CO2 emission to less than a half of the conventional one at the same time, the R and D started in fiscal 1997. As to the study of a high efficiency hybrid power system, conducted were the prediction of fuel consumption performance of the system proposed, evaluation of element technology using hybrid simulator, evaluation experiment on a new hybrid vehicle, and grasp of overseas trends. In relation to the development of hybrid vehicles, the following were studied: methanol fuel cell loading hybrid vehicle, CNG engine loading hybrid vehicle, CNG ceramic engine loading hybrid truck, CNG lean burn engine loading hybrid truck, LNG engine loading hybrid bus, and DME engine loading hybrid bus. Besides, a survey on synthetic fuel and the related survey were carried out. 17 refs., 185 figs., 101 tabs.

National Security and the Right to Information in Europe

DEFF Research Database (Denmark)

Jacobsen, Amanda Lynn

2013-01-01

Full text available at: http://cast.ku.dk/pdf/National_Security_and_the_Right_to_Information.pdf/......Full text available at: http://cast.ku.dk/pdf/National_Security_and_the_Right_to_Information.pdf/...

Organizational Analysis of the United States Army Contracting Command-Kuwait

National Research Council Canada - National Science Library

Orr, Kristine R

2008-01-01

This study of the U.S. Army Contracting Command - Kuwait (USACC-KU) used an organizational systems framework to analyze factors related to strategy structure, processes and results experienced at USACC-KU during 2006-2008...

Siim Nestor soovitab : Tallinn Doom Night vol.1. Odessa Pop / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2006-01-01

Soome doom-metal ansambel Reverend Bizarre üritusel "Tallinn Doom Night vol.1" 8. dets. klubis Rockstar's. Indipoppi viljelev Rootsi duo My Darling Yoy! üritusel "Odessa Pop" 9. dets. Tallinnas klubis KuKu

Ginsbergi vennaskond, värskelt ühendatud / Marko Mägi

Index Scriptorium Estoniae

Mägi, Marko

2005-01-01

Poetry slami laadis luuleõhtul "Poesie United" esinesid 21. mail KuKu klubis Wehwalt Koslovsky, Tobias Hoffmann (Saksamaa), Ben Porter Lewis, Milo Martin (USA), Antoine Faure (Prantsusmaa), fs, Asko Künnap, Jürgen Rooste

Uudised : "Odessa Pop uuel ringil". Neoandertals jõuab plaadile. "Xanadu" Hollandis muusikalilaval

Index Scriptorium Estoniae

2007-01-01

Kontsertõhtust sarja Odessa Pop raames 17. veebr. Tallinnas baar-klubis Juuksur. Ultrabrutaalse deathmetali duo Neoandertals heliplaadi "Neanderthals Were Master Butchers" esitluskontserdist 17. veebr. Tallinnas KuKu klubis. 1980. aastal valminud diskofilmi "Xanadu" muusikaliversioonist

Fiscal 1997 report on the results of the research on the development of high-performance industrial furnaces. Volume 2; 1997 nendo seika hokokusho (koseino kogyoro no kaihatsu ni kansuru kenkyu seika hokokusto)

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

A R and D was conducted for the purpose of developing remarkable energy conservation/CO2 reduction technologies. In fiscal 1997, the five working groups about the following were formed to have animated discussions: the basic research, heating furnace, heat treatment furnace, melting furnace, and tubular heating furnace. The research subjects are the pursuit/deepening of the base study on flame structures in high temperature air combustion, critical heat recovery of the system in high temperature air combustion, examination of the basic common technology on the ultimate optimization of the material heat transfer, construction of general-purpose experimental database, setting-up of design standards for general-purpose high-performance industrial furnaces, presentation of examples of the actual trial design based on the design standards and feasibility study of the practical application, preparatory support of the field test project on high-performance industrial furnaces for commercialization/diffusion/spread, etc. Further, how to arrange the developmental results was studied to make the results of the project useful for study of the prompt commercialization. 442 refs., 1444 figs., 311 tabs

Fiscal 1997 report on the results of the research on the development of high-performance industrial furnaces. Volume 3; 1997 nendo seika hokokusho (koseino kogyoro no kaihatsu ni kansuru kenkyu seika hokokusto)

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

A R and D was conducted for the purpose of developing remarkable energy conservation/CO2 reduction technologies. In fiscal 1997, the five working groups about the following were formed to have animated discussions: the basic research, heating furnace, heat treatment furnace, melting furnace, and tubular heating furnace. The research subjects are the pursuit/deepening of the base study on flame structures in high temperature air combustion, critical heat recovery of the system in high temperature air combustion, examination of the basic common technology on the ultimate optimization of the material heat transfer, construction of general-purpose experimental database, setting-up of design standards for general-purpose high-performance industrial furnaces, presentation of examples of the actual trial design based on the design standards and feasibility study of the practical application, preparatory support of the field test project on high-performance industrial furnaces for commercialization/diffusion/spread, etc. Further, how to arrange the developmental results was studied to make the results of the project useful for study of the prompt commercialization. 442 refs., 1444 figs., 311 tabs.

Fiscal 1997 report on the results of the research on the development of high-performance industrial furnaces. R and D of high-performance industrial furnaces, etc; 1997 nendo seika hokokusho (koseino kogyoro no kaihatsu ni kansuru kenkyu seika hokokusho). Koseino kogyoro nado ni kansuru kenkyu kaihatsu

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

A R and D was conducted for the purpose of developing remarkable energy conservation/CO2 reduction technologies. In fiscal 1997, the five working groups about the following were formed to have animated discussions: the basic research, heating furnace, heat treatment furnace, melting furnace, and tubular heating furnace. The research subjects are the pursuit/deepening of the base study on flame structures in high temperature air combustion, critical heat recovery of the system in high temperature air combustion, examination of the basic common technology on the ultimate optimization of the material heat transfer, construction of general-purpose experimental database, setting-up of design standards for general-purpose high-performance industrial furnaces, presentation of examples of the actual trial design based on the design standards and feasibility study of the practical application, preparatory support of the field test project on high-performance industrial furnaces for commercialization/diffusion/spread, etc. Further, how to arrange the developmental results was studied to make the results of the project useful for study of the prompt commercialization. 442 refs., 1444 figs., 311 tabs.

Siim Nestor soovitab : Back2Bass Helsinki. Teenage Kicks / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2002-01-01

Soome trummi-ja-bassi klubi Back2Bass 28. septembril KuKu klubis Tallinnas. 28. sept. Von Krahlis toimuvast live-üritusest Teenage Kicks, kus ansambel Claire's Birthaday esitleb ka oma uut singlit "Do You Remember"

Uudised : Depeche Mode'i maailmaturnee peaaegu välja müüdud. Easy Listening esitleb : Villu Tamme diskorina

Index Scriptorium Estoniae

2001-01-01

Ans. Depeche Mode maailmaturnee "Exciter" Euroopa ja Ameerika kontserdid on peaaegu välja müüdud. 11. mail KuKu klubis toimuval muusikapeol sarjas 'The Easy Listening Sessions' astub diskorina üles V.Tamme

Chance Discovery with Data Crystallization: A Basic Research for Discovering Unobservable Events

Science.gov (United States)

2006-05-10

Ohsawa y School of Engineering, The University of Tokyo, 7-3-1 Hongo , Bunkyo-ku, 113-8563, Japan y.ohsawa@gmail.com Received 17 June 2005 This paper...this study as the software developer of data crystallization. y School of Engineering, The University of Tokyo, 7-3-1 Hongo , Bunkyo-ku, Tokyo 113-8653...University of Tokyo, 7-3-1 Hongo , Bunkyo-ku, Tokyo, 113-8563 Japan, ohsawa@q.t.u-tokyo.ac.jp Abstract. There are invisible events which play an important role

Gridded 5-day mean sea surface height anomaly and significant wave height from Jason-1 and OSTM/Jason-2 satellites (NODC Accession 0065055)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains the gridded 5-day mean sea surface height anomaly (SSHA) and Ku Band significant wave height (SWH-KU) observed from Jason-1 and OSTM/Jason-2...

Siim Nestor soovitab : Eddie Amador Vibe'i neljandal sünnipäeval. Aasta hiphop.ee-d / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2002-01-01

Ameeriklane Eddy Amador külas Vibe neljandal sünnipäeval 11.okt. Internetiaadressi www.hiphop.ee esimese aastapäeva tähistamisest Tartus Club-Tallinnas 11. okt. ja Tallinnas KuKu klubis 12. oktoobril

KU24 METAR

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

Ku dydaktyce afirmatywnej

Directory of Open Access Journals (Sweden)

Monika Rogowska-Stangret

2016-11-01

Full Text Available "Towards affirmative didactics" The article “Towards affirmative didactics” is a description of a project reflecting on the experience of teaching and learning in a systematic and complex manner. It is also an effort to define the conditions that could encourage values such as inclusiveness, tolerance, mindfulness, joint responsibility, as well as engagement in and support for actions undertaken to strengthen the above-mentioned values. The project is a work in progress and it consists of two parts. The first one is critical and it describes the given situation by pointing the main threats involved. The second – affirmative one – was created with the effort to develop positive guidelines that would encourage transformations in the approach to didactics.

Siim Nestor soovitab : Turbodisko. Camouflage / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2006-01-01

Üritusest "Turbodisko" 2. dets. Tallinnas KuKu klubis, indieansambli Bad Apples heliplaadi "When Colours Become Day and Night" (plaadifirmalt Seksound) esitluskontserdist. Saksamaa futuelektroonika ansambli Camouflage albumi "Relocated" esitluskontserdist 3. dets klubis Hollywood (kontserti soojendab läti ansambel STandArt)

Report of results of contract research. 'Research on magneto hydrodynamic (MHD) generation'; MHD hatsuden system no kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1980-03-01

Examination was conducted in detail on an MHD generation system by coal combustion, with the results reported. Concerning a gas table calculation program in coal combustion, it was prepared assuming 100% slag removal ratio in the combustor as the primary approximation. A combustor for MHD generation needs to efficiently burn fuel using high temperature pre-heated air as the oxidant, to fully dissociate/electrolytically dissociate seed, and to supply to the generation channel a high speed combustion gas plasma having a high electrical conductivity which is required for MHD generation. This year, an examination was conducted on technological problems in burning coal in an MHD combustor. As for the NOx elimination system in an MHD generation plant, an examination was made if the method studied so far in MHD generation using heavy oil as the fuel is applicable to coal. Also investigated and reviewed were various characteristics, change in physical properties, recovery method, etc., in a mixed state of seed and slag in the case of coal combustion MHD. (NEDO)

Fiscal 1996 report on technological results. R and D on micromachine technology; 1996 nendo micromachine gijutsu no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

Researches on basic element technology of micromachines are conducted that operate autonomously in a narrow small part in a complicated apparatus or in vivo. The areas of activity are (1) research on micromachine systems, (2) a subminiature liquid synthesizing system, and (3) comprehensive investigation and research. In (1), the researches were carried out on the miniaturization and functional combination of a micro laser catheter and a micro tactile sensor catheter, which are the primary components of a coeliac diagnostic and therapeutic system, a 'micro catheter for cerebral blood vessel/treatment', as a micromachine system in the medical field. In (2), R and D was conducted on a system element technology assuming it contributed to a subminiature liquid synthesizing system capable of preparing various liquids including pharmaceuticals accurately with a trace amount. In (3), examination was made on the application area of a micromachine system with priority given to a medical field and also on technological subjects to be tapped, as well as on the contents of (2), with a device installed for evaluating the operating characteristic of a distribution type fluid actuator as needed for the development. (NEDO)

Achievement report for fiscal 1998 on Welfare Technosystem Oita research and development; 1998 nendo Walfare technosystem kenkyu kaihatsu (Oita) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

Before a welfare facility is introduced into a home, studies should be sufficiently conducted about the circumstances of daily life, not only of the aged user but also of the family members who take care of the aged user. In the development of handrails, it is found that plastic foam containing wood flour presents the feel of wood, easy to bend when heated. Materials are investigated for flexural strength, and ABS (acrylonitrile butadiene styrene) is chosen as the suitable material. As for devices to assist toileting, a system is developed under which the patient's legs are raised. Since there are arguments for and against the operation itself of mechanically raising a patient's legs, however, efforts will continue to develop much advanced systems. In the study of walk and transfer behavior, a toilet frame mockup is designed and manufactured, which is for collecting data. In an preliminary experiment, tests are conducted about a transfer from a chair to another and, assuming a physically handicapped patient, a transfer from a wheelchair. The data thus collected are to be subjected to analysis and image processing by the use of measuring sensors. Development is described of interfaces, for use in devices to serve as information mediums, which consider the cognition characteristics of the aged people. As for the development of devices for communication, environmental control, and display, their basic operation and feasibility are discussed. Actualities of costumes for physically handicapped people are also investigated, and development is carried out as required. (NEDO)

Summarized achievement report on the Sunshine Project in fiscal 1979. Hydrogen energy; 1979 nendo sunshine keikaku seika hokokusho gaiyoshu. Suiso energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1980-04-01

This paper summarizes the achievement report on the Sunshine Project in fiscal 1979 for hydrogen energy research. In hydrogen manufacturing technologies, the paper describes improvement in membrane performance and discussions on electrode materials in high temperature and pressure electrolysis. In the thermo-chemical method, hydrolysis of iron bromide (II) in the iron system cycle was compared to three kinds of reaction patterns corresponding to phase change, and evaluation was given as the hydrogen generating reaction. In the iodine system the first stage oxidation and reduction reaction of MgO-I{sub 2} was subjected to a continued experiment by using a batch autoclave. Discussions were continued on device materials for the iodine cycle. In the light irradiation electrolytic method for the mixed cycle, the light intensity was experimented at a force 12 times greater than that of the solar beam, and a reaction rate of 80% was achieved. Raising the temperature causes the reaction rate to decline, but it can be supplemented by raising the light intensity. A heat diffusion column was found effective in HI decomposition (hydrogen acquisition). For hydrogen transportation and storage, researches are continued on metal hydrides. In hydrogen utilization technologies, combustion, fuel cells (using high temperature solid and alkaline aqueous solution electrolytes), and hydrogen engines are studied. This paper also describes studies on hydrogen safety assuring measures and energy systems. (NEDO)

Sunshine Program for fiscal 1981. Research on direct coal-liquefaction reactor; 1981 nendo sekitan chokusetsu ekika hannoki no kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1982-04-17

The research aims to develop a new-type high-efficiency reactor for direct coal-liquefaction from a reaction engineering viewpoint and, at the same time, to settle problems expected to occur in industrializing this technique. The research items are Item 1 on the analysis of characteristics of a tubular continuous reactor and Item 2 on high-activity catalysts. Under Item 1, on the assumption that liquefaction reaction is dominantly a reaction in a continuous phase, a reactor is selected in consideration of homogeneous-phase reaction, and operating conditions are set according to the selected reactor and to the liquefaction reaction characteristics. A somewhat large Re number is assigned to the paste. Since the hydrogen partial pressure lowers with the progress of reaction, hydrogen is resupplied at the middle of the reaction tube. The volume of gas increases toward the final stage of reaction, decreasing the continuous phase holdup. Since it is undesirable to let the decrease stand, a vapor-liquid separator is provided for the control of the gas volume. In this fiscal year, investigation is conducted into details so as to determine if the reactor is equipped with intended characteristics. Under Item 2, the iron ore is deemed to be the most promising as disposal catalysts for liquefaction. In this fiscal year, studies are conducted to disclose the activity etc. of catalysts of this type. (NEDO)

Achievement report for fiscal 1998 on Welfare Technosystem Oita research and development; 1998 nendo Walfare technosystem kenkyu kaihatsu (Oita) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

Before a welfare facility is introduced into a home, studies should be sufficiently conducted about the circumstances of daily life, not only of the aged user but also of the family members who take care of the aged user. In the development of handrails, it is found that plastic foam containing wood flour presents the feel of wood, easy to bend when heated. Materials are investigated for flexural strength, and ABS (acrylonitrile butadiene styrene) is chosen as the suitable material. As for devices to assist toileting, a system is developed under which the patient's legs are raised. Since there are arguments for and against the operation itself of mechanically raising a patient's legs, however, efforts will continue to develop much advanced systems. In the study of walk and transfer behavior, a toilet frame mockup is designed and manufactured, which is for collecting data. In an preliminary experiment, tests are conducted about a transfer from a chair to another and, assuming a physically handicapped patient, a transfer from a wheelchair. The data thus collected are to be subjected to analysis and image processing by the use of measuring sensors. Development is described of interfaces, for use in devices to serve as information mediums, which consider the cognition characteristics of the aged people. As for the development of devices for communication, environmental control, and display, their basic operation and feasibility are discussed. Actualities of costumes for physically handicapped people are also investigated, and development is carried out as required. (NEDO)

Fiscal 1997 report on technological results. R and D on micromachine technology; 1997 nendo micromachine gijutsu no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

Researches on basic element technology of micromachines are conducted that operate autonomously in a narrow small part in a complicated apparatus or in vivo. The areas of activity are 1. research on micromachine systems and 2. comprehensive investigation and research. In 1, (1) a micro catheter and (2) a micro tactile sensor catheter were studied while, in 2, basic technology and technological problems, particularly those problems concerning sophistication in the medical field were examined and studied. Further, in (1), with the purpose of developing a micro laser catheter for diagnosis/treatment, researches were implemented on the technology of integrating micro laser and an optical fiber which were components of the catheter and on the fabrication of the fine diameter of a micro catheter as well as on the characteristical improvement of the base body on which minute elements were mounted. In (2), characteristical improvement and sophistication were carried out for a high sensitivity contact pressure sensor and a diagnostic tactile sensor, which are mounted on a catheter having a positive bulge control mechanism for insertion into body cavity, with a sensor probe manufactured experimentally. (NEDO)

Achievement report for fiscal 1998 on Welfare Technosystem Kamogawa research and development; 1998 nendo Welfare technosystem kenkyu kaihatsu (Kamogawa) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

Opinions were heard from residents about residences designed for aged people. According to their physical conditions, residents of advanced age may be classified into four groups, that is, 'Aged person who is self-reliant,' 'Patient who can walk using a walking stick or handrail, with a caregiver,' 'Patient who rides a wheelchair, with a caregiver,' 'Patient who is bedridden, with a caregiver.' The collected data will be effectively utilized in the future for planning residences that respect an advanced age. The difference between the four groups ensues from difference in human relationship in the residences and in movement and caregiving in daily life. When a residence in which a patient lives with the family is set forth for premise, what is important is to consider the request of the patient that the residence be functionally perfect and, at the same time, attention must be paid to the psychological desire that the residence be equipped with what a home is generally supposed to offer. Emerging increasingly definite out of the research conducted this fiscal year are policies towards the development of, and the feasibility of, a remotely controlled medical assistance system with patients allowed to stay at home and terminal equipment therefor. An experimentally constructed movement assistance unit is tested at Welfare Technohouse Kamogawa, and findings are obtained about the safe use of a barrier-free condominium thanks to such functions as motor-assisted walking, assistance for standing-up, collision avoidance, etc. (NEDO)

Achievement report for fiscal 1998 on Welfare Technosystem Osaka research and development; 1998 nendo Walfare technosystem kenkyu kaihatsu (Osaka) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

Old people's need for illumination is sufficiently covered by the JIS-stipulated illumination range. Although the minimum safe limit for the aged is serval times higher than that of the young, yet electricity saving may still be enhanced by proper adjustment without spoiling amenity for the aged. A hard floor puts a burden on the knees. The degree of fatigue due to the use of footgear is dependent not only on the shape but also on the user's habit. The transfer equipment seat surface height, if improperly set, oppresses the user psychologically and physiologically, the burdens heavier when the seat is lower. As compared with transfer aboard the wheelchair, a horizontal movement on the seat surface by the use of arms (fore and aft, right and left) demands less physiological burden. The above-said matters are taken into consideration when designing transfer equipment. As for emergency information systems, it is necessary to study seat pressure distribution, change in body temperature in a bath, change in blood pressure on the stool, and to develop a pulse counting simulator. A life space simulator and welfare utensil fitness evaluation are discussed. Also discussed are the development of a simulator for the comprehensive evaluation of motorized wheelchairs for their capability to encourage self-reliance, monitoring systems, and supervising/reporting systems (toilet irregularity annunciators etc.). (NEDO)

Achievement report for fiscal 1998. Development of environmentally friendly universal controller; 1998 nendo kankyo taio universal controller no kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-05-01

Research and development was carried out for an 'environmentally friendly universal controller (UC)' which would be 'user-friendly and energy-saving' when used with electrical home appliances. An environmentally friendly UC is a remote controller which utilizes an infrared two-way communication function and becomes capable of dealing with any appliance upon downloading remote control information from the appliance involved. Studies were conducted for the development of controller hardware and software therefor and for the development of electrical home appliances to correspond to their specifications. In relation with energy conservation, studies centered about reduction in power consumption while on standby and unnecessary power consumption. As the result, an environmentally friendly UC protocol was newly developed and, based on the protocol, plural numbers of controllers, appliances, and network gateways were tentatively fabricated, and were examined for their function, behavior, and operational interchangeability. Systems aiming at reducing power consumption while on standby and unnecessary power consumption were built by way of experiment, and were checked for performance. It was then concluded that a reduction of several billion kWh might be achieved. (NEDO)

Fiscal 1998 'Plant Biotechnology in the 21st Century' workshop report; '21 seiki no shokubutsu biotechnology' workshop 1998 nendo seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

The workshop was opened with the opening remarks by Yamada (President of Nara Institute of Science and Technology (NIST)), the overview of plant biotechnology in the 21st century from academia by Shinmyo (Professor of NIST), and the overview of such technology from Ministry of International Trade and Industry by Katao (Chief of Chemical Industry Division). Lectures and discussions of various topics were conducted for 2 days as follows. The effectiveness of a genomic DNA array method for obtaining the genes for switching genes according to daytime, nighttime, drying, salt, high temperature and low temperature for every plant. Current transfer technology of large DNA fragments into plant cell nuclei and chloroplast. Biological evaluation of the physiological functions and complex stress tolerance capacity transformed by transferring complex stress tolerance genes and useful genes for productivity improvement and value addition. Discussion was also held on the importance of a basic research for biotechnology in the 21st century. (NEDO)

Achievement report for fiscal 1998. Development of next-generation chemical process technologies; 1998 nendo jisedai kagaku process gijutsu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

Researches are conducted on the development of a technology of catalytic cracking of naphtha, technology relative to selective oxidation reaction of saturated hydrocarbons, process technology utilizing solid-phase reaction field, and a process technology utilizing novel reaction mechanism. Also, a survey is conducted on chemical processes of the next generation. Concerning the catalytic cracking of naphtha, reaction systems are roughly divided into two groups by whether or not they proceed in the presence of oxygen. As for rare earth oxide catalyst systems and zeolitic catalyst systems, their performance is confirmed and their reaction mechanisms are estimated. Concerning the selective oxidation reaction of hydrocarbons, studies are made to enhance the performance of catalytic systems that have been selected in researches conducted in the past. Concerning the process of solid phase reaction field utilization, the project is summarized and a concept is constructed of 'solid phase catalyst.' Concerning novel reaction mechanism utilizing process technologies, development is discussed of a novel process using membrane reactor, a highly functional hydrogen permeable membrane, a low-temperature dehydrogenation catalyst, etc., and a dehydrogenation membrane reactor is experimentally built. (NEDO)

Achievement report for fiscal 1998 on Welfare Technosystem Ube research and development; 1998 nendo Walfare technosystem kenkyu kaihatsu (Chiba) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

Research is conducted into burdens to be imposed on the nurse and the attended person during nursing activities and into measures to lighten such burdens, and basic data are collected about physical strains to be produced when raising a patient on the bed, pushing a wheelchair, and about stool shapes appropriate for the aged. In a study about escape from a disaster, many shortcomings are discovered requiring remedy, such as difference in height and doors failing to open in case of power outage. For communication in case of emergency, it is concluded that the most promising method will be a combination of a mobile computer and handyphone system. A photoelectric pulse wave monitoring system and an acceleration sensor-equipped water bed, on which the patient lies unrestrained for the measurement of heartbeats and respiration waveforms, are developed. A concept wheelchair is under development which will be a 2-motor 4-wheel design, to be equipped with a steering wheel and a vertically movable seat which will facilitate boarding and alighting. Many projects are in store for fiscal 2001, which include the development of a system to ensure sitting position stability and an automatic pressure belt for the prevention of orthostatic hypotension. A multiple function bed is under development for domestic use, which will be provided with a discharge system (automatic toilet) whose mechanism is quite sophisticated. (NEDO)

Report on the coal energy achievements in the Sunshine Project in fiscal 1988; 1989 nendo sunshine keikaku seika hokokusho. Sekitan energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1989-07-01

This paper describes the achievements in the Sunshine Project in fiscal 1988 in studies on coal liquefaction. The liquefaction mechanisms may be conceived to work in the following three manners: radicals produced by spontaneous bond and cleavage of coal molecules draw out hydrogen from the donor and stabilize it; bond, cleavage and stabilization due to hydrogenation from the donor to coal aromatic nucleus; and cleavage and stabilization due to work of active hydrogen discharged from the donor. How these mechanisms contribute to composition of the products depends on coals, liquefying conditions, and reactivity of the donor. Selection of the donor solvent requires design by which high liquefaction yield can be obtained with small amount of solvent. The oil plus asphaltene yield could have been approached to the ideal value through the moving liquefaction of gaseous phase hydrogen and catalyst-free hydrogen, with the 4HFL to coal ratio at 1.5 to 1. The gaseous phase hydrogen was found effective in reducing 4HFL. Liquefaction residual oil manufactured from Morwell brown coal subjected to BCL was hydrogenated in single stage or two stages to compare and discuss the solvent sorting constituents, reactivity of CLVR, and how the catalytic activity deteriorates. The cause for the catalyst deterioration was elucidated, and considerations were given on catalysts and reaction conditions for lightening and refining of CLVR. (NEDO)

Achievement report for fiscal 1998. Development of next-generation chemical process technologies; 1998 nendo jisedai kagaku process gijutsu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

Researches are conducted on the development of a technology of catalytic cracking of naphtha, technology relative to selective oxidation reaction of saturated hydrocarbons, process technology utilizing solid-phase reaction field, and a process technology utilizing novel reaction mechanism. Also, a survey is conducted on chemical processes of the next generation. Concerning the catalytic cracking of naphtha, reaction systems are roughly divided into two groups by whether or not they proceed in the presence of oxygen. As for rare earth oxide catalyst systems and zeolitic catalyst systems, their performance is confirmed and their reaction mechanisms are estimated. Concerning the selective oxidation reaction of hydrocarbons, studies are made to enhance the performance of catalytic systems that have been selected in researches conducted in the past. Concerning the process of solid phase reaction field utilization, the project is summarized and a concept is constructed of 'solid phase catalyst.' Concerning novel reaction mechanism utilizing process technologies, development is discussed of a novel process using membrane reactor, a highly functional hydrogen permeable membrane, a low-temperature dehydrogenation catalyst, etc., and a dehydrogenation membrane reactor is experimentally built. (NEDO)

Fiscal 1982 Sunshine Project research report. Development of bore hole measurement technology; 1982 nendo koseinai sokutei gijutsu no kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1983-03-01

This report summarizes the fiscal 1982 research result on development of measuring instruments for information collection in geothermal bore holes under high-temperature high-pressure conditions. In development of high-temperature well logging cable, the new TFE Teflon insulated well logging cable (3,300m) applicable up to 320 degreesC was prepared. In development of digital well logging system, the data analyzer for edition and analysis of recorded digital logging data, the depth panel with an automatic depth correction mechanism, and the fiber optics controller were prepared. Preparation of the whole digital well logging system was thus completed. Field test was made at 3 wells in 2 geothermal areas. At the test well in Nigori-Gawa area, Hokkaido, performance test was made on the digital well logging system, TFE Teflon well logging cable, and improved winch completed in this fiscal year. At the same time, the radioactive well logging test using radioactive isotope showed the satisfactory result except neutron well logging. (NEDO)

Report of overseas dispatch of Japanese researchers by RITE in fiscal 1995; 1995 nendo RITE kokunai kenkyusha kaigai haken seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-12-31

This is a report of overseas dispatch of domestic researchers by the Research Institute of Innovative Technology for the Earth (RITE). When the CO2 reduction effect in the air is evaluated by the chemical fixation of CO2, not only the reduction but also the total reduction of CO2 emission by introducing the countermeasure technology should be investigated. Measures for reduction were evaluated by the LCA technique in Austria, and related information was collected in Europe. The promotion of international cooperation for the CO2 storage in the ocean, and the economy and environmental harmony of the whole system including separation, recovery, transportation of CO2, and its supply into the ocean were investigated in Massachusetts Institute of Technology. Based on the biological synthesis and decomposition of chitin and chitosan (chitin matter circulation) which are components of shell of insects and shellfish, the fixation of CO2 and development of new materials in the hydrosphere were investigated in Norway. To clarify the reaction mechanism and critical factors of the yield of the rhenium carbonyl complex, a CO2 photoreduction catalyst, a relationship between the change of electronic state of complex due to chemical modification and the infrared absorption was analyzed in the United Kingdom. 26 refs., 40 figs., 12 tabs.

Environmental radiation status in Bunkyo-ku, Tokyo, after the TEPCO Fukushima Dai-ichi Nuclear Power Plant disaster

International Nuclear Information System (INIS)

Ebisawa, Takao; Hirose, Seiichi; Furuta, Etsuko; Kusama, Keiji; Iimoto, Takeshi

2013-01-01

Bunkyo-ku is located in the eastern part of the metropolitan area of Tokyo, Japan, and is roughly 220 km south of the TEPCO Fukushima Dai-ichi Nuclear Power Plant. The population of the city was 201,079 as of August 1, 2012, and its area is 11.31 km"2. The local government has officially been surveying the environmental radiation status after the disaster in response to numerous requests from its citizens. The radiation surveillance in this area has been technically guided by radiation protection specialists. The two main targets for surveillance are (1) the ambient radiation dose (microsieverts per hour) at all the school yards, public parks, and representative measurement points selected by the local government, and (2) the specific radioactivity (becquerels per kilogram) present in school lunch. These data have been reported to the citizens through the city website as well as in a bi-monthly report in the public relations magazine of the local government. This report presents the background status and technical information of the related activities, as well as the measured environmental radiation data. The ambient radiation dose in the city has been surveyed since July 2011. In the 1st period of surveillance (from July to August, 2012), over a total of 304 measurement points, the highest recorded value of the ambient radiation dose was 0.22 μSv h"-"1 at the height of 1 m from the ground, the lowest was 0.05 μSv h"-"1, and the average was around 0.09 μSv h"-"1. These values include the natural background dose rate detected by the energy compensation type surveymeters. In the most recent surveillance records, the maximum value recorded was 0.10, the minimum was 0.05, and 0.07 μSv h"-"1 was the average value. The specific radioactivity of drinking water has been monitored at local purification plants since the accident occurred. No water sample supplied to the city has exceeded the national limits for intake dose. The specific radioactivity of school lunch was

Variability of selected indicators of physical activity in a randomized sample of the Czech population between the years 2003–2006: Results from the short and long self administered format of the IPAQ questionnaire [Variabilita vybraných indikátorů pohybové aktivity u randomizovaných souborů české populace v letech 2003–2006: Výsledky z krátké a dlouhé administrativní verze IPAQ dotazníku

Directory of Open Access Journals (Sweden)

Erik Sigmund

2009-06-01

MET–minutes/week for walking and sitting on working days. The long self administered format of the IPAQ provides more detailed results on PA. However, the value of total PA is significantly higher than that obtained using the short self administered format of the IPAQ. In order to obtain more exact data on PA, we recommend using the long self administered format of the IPAQ simultaneously with pedometer or accelerometer PA monitoring. [VÝCHODISKA: Snaha o získání celosvětově srovnatelných výsledků o úrovni pohybové aktivity (PA různých populačních skupin vyústila ve vytvoření a standardizaci Mezinárodního dotazníku o pohybové aktivitě (IPAQ. CÍLE: Hlavním cílem této studie bylo zjistit variabilitu vybraných proměnných PA ze dvou ekvivalentních forem českých administrativních verzí IPAQ dotazníku aplikovaných u randomizovaných souborů české populace v letech 2003–2006. METODIKA: Ve studii byla použita data od 11277 obyvatel České republiky (5683 žen a 5594 mužů ve věku 15–69 let. Krátká administrativní verze IPAQ byla u 2066 žen a 1976 mužů aplikována na podzim 2003 a jaře 2004. Dlouhá administrativní verze IPAQ (resp. jako součást ANEWS dotazníku byla použita u 1688 žen a 1689 mužů na podzim 2004 a jaře 2005 (resp. u 1929 žen a 1929 mužů na podzim 2005 a jaře 2006. Jednotlivé soubory účastníků byly randomizovány podle pohlaví, věku a velikosti místa bydliště v souladu s údaji Českého statistického úřadu (www.czso.cz. VÝSLEDKY: Mezi samostatně aplikovanou dlouhou verzí IPAQ a ANEWS dotazníkem (obsahujícím dlouhou verzi IPAQ nenacházíme u mužů ani žen výrazné rozdíly v MET–minut/týden v celkové PA, transportní PA, PA v zaměstnání a chůzi. U krátké verze IPAQ zaznamenáváme u mužů i žen nižší hodnoty celkové PA (p < 0,0001 než v dlouhé verzi IPAQ (resp. ANEWS dotazníku. Bez rozdílu mezi jednotlivými ekvivalentními formami IPAQ dotazníku jsou

Mycobacterium tuberculosis and Mycobacterium marinum non-homologous end-joining proteins can function together to join DNA ends in Escherichia coli.

Science.gov (United States)

Wright, Douglas G; Castore, Reneau; Shi, Runhua; Mallick, Amrita; Ennis, Don G; Harrison, Lynn

2017-03-01

Mycobacterium tuberculosis and Mycobacterium smegmatis express a Ku protein and a DNA ligase D and are able to repair DNA double strand breaks (DSBs) by non-homologous end-joining (NHEJ). This pathway protects against DNA damage when bacteria are in stationary phase. Mycobacterium marinum is a member of this mycobacterium family and like M. tuberculosis is pathogenic. M. marinum lives in water, forms biofilms and infects fish and frogs. M. marinum is a biosafety level 2 (BSL2) organism as it can infect humans, although infections are limited to the skin. M. marinum is accepted as a model to study mycobacterial pathogenesis, as M. marinum and M. tuberculosis are genetically closely related and have similar mechanisms of survival and persistence inside macrophage. The aim of this study was to determine whether M. marinum could be used as a model to understand M. tuberculosis NHEJ repair. We identified and cloned the M. marinum genes encoding NHEJ proteins and generated E. coli strains that express the M. marinum Ku (Mm-Ku) and ligase D (Mm-Lig) individually or together (LHmKumLig strain) from expression vectors integrated at phage attachment sites in the genome. We demonstrated that Mm-Ku and Mm-Lig are both required to re-circularize Cla I-linearized plasmid DNA in E. coli. We compared repair of strain LHmKumLig with that of an E. coli strain (BWKuLig#2) expressing the M. tuberculosis Ku (Mt-Ku) and ligase D (Mt-Lig), and found that LHmKumLig performed 3.5 times more repair and repair was more accurate than BWKuLig#2. By expressing the Mm-Ku with the Mt-Lig, or the Mt-Ku with the Mm-Lig in E. coli, we have shown that the NHEJ proteins from M. marinum and M. tuberculosis can function together to join DNA DSBs. NHEJ repair is therefore conserved between the two species. Consequently, M. marinum is a good model to study NHEJ repair during mycobacterial pathogenesis. © The Author 2016. Published by Oxford University Press on behalf of the UK Environmental Mutagen

Fiscal 1998 research report on information structuring technology for advanced use of NEDO research reports; 1998 nendo NEDO seika hokokusho nado no kodo riyo ni hitsuyona joho kozoka gijutsu no chosa hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

Research was made on electronic NEDO research reports corresponding to the recent extending trend of Internet. The logical structure and electronic format of NEDO research reports as document were first studied considering document disclosure and reuse of technical information. HTML as electronic format for Internet is unfortunately insufficient to describe the logical structure of NEDO research reports because of HTML's restricted description. Thus, XML which is being watched because of its possible definition of original tags was studied in place of HTML. As a result, because at present preparation of standards on XML and a compatibility for implementation of standards are insufficient, it was concluded that construction of the electronic document system for NEDO research reports is still too early. However, since adoption of XML as document format is important from the viewpoint of a property succession on Internet, preparation of the XML format file of NEDO research reports is essential. (NEDO)

ВПЛИВ ПОРОГОВИХ УМОВ ITU НА ЕФЕКТИВНІСТЬ ВИКОРИСТАННЯ ГЕОСТАЦІОНАРНОЇ ОРБІТИ В Ku ДІАПАЗОНІ

OpenAIRE

Kolomytsev, M. A.; Lipatov, A.; Synepolski, Y.

2014-01-01

The article is devoted to the estimation of threshold values, regulated by ITU, which determine the necessity of satellite networks coordination in the Ku band. Maximum capacity of geostationary orbit (GEO) for different diameters ofearth station antennas, operating in standard DVB-S2 accounting the limitations caused by the parameters of existing satellite networks equipment, is determined. Optimal values of satellites’ orbital separation, relative increase in allowablenoise / and the signal...

Inhibiting DNA-PKCS radiosensitizes human osteosarcoma cells

International Nuclear Information System (INIS)

Mamo, Tewodros; Mladek, Ann C.; Shogren, Kris L.; Gustafson, Carl; Gupta, Shiv K.; Riester, Scott M.; Maran, Avudaiappan; Galindo, Mario; Wijnen, Andre J. van; Sarkaria, Jann N.; Yaszemski, Michael J.

2017-01-01

Osteosarcoma survival rate has not improved over the past three decades, and the debilitating side effects of the surgical treatment suggest the need for alternative local control approaches. Radiotherapy is largely ineffective in osteosarcoma, indicating a potential role for radiosensitizers. Blocking DNA repair, particularly by inhibiting the catalytic subunit of DNA-dependent protein kinase (DNA-PK CS ), is an attractive option for the radiosensitization of osteosarcoma. In this study, the expression of DNA-PK CS in osteosarcoma tissue specimens and cell lines was examined. Moreover, the small molecule DNA-PK CS inhibitor, KU60648, was investigated as a radiosensitizing strategy for osteosarcoma cells in vitro. DNA-PK CS was consistently expressed in the osteosarcoma tissue specimens and cell lines studied. Additionally, KU60648 effectively sensitized two of those osteosarcoma cell lines (143B cells by 1.5-fold and U2OS cells by 2.5-fold). KU60648 co-treatment also altered cell cycle distribution and enhanced DNA damage. Cell accumulation at the G2/M transition point increased by 55% and 45%, while the percentage of cells with >20 γH2AX foci were enhanced by 59% and 107% for 143B and U2OS cells, respectively. These results indicate that the DNA-PK CS inhibitor, KU60648, is a promising radiosensitizing agent for osteosarcoma. - Highlights: • DNA-PKcs is consistently expressed in human osteosarcoma tissue and cell lines. • The DNA-PKcs inhibitor, KU60648, effectively radiosensitizes osteosarcoma cells. • Combining KU60648 with radiation increases G2/M accumulation and DNA damage.

Homme kell 19 algab EKA galeriis Norra eri väljapanek "(A guide to) constructing the universe"

Index Scriptorium Estoniae

2005-01-01

Kuraatoriduo Rakett koosseisus Karolin Tampere ja Ase Lovgren norra noorema kunsti ja muusikaga.12. III toimuva norra video- ja performance'i-õhtu esinejad loetletud, vt. ka lk. 24. Projekti II osa 19. märtsil Ku-Ku klubis (bändid ja dj-d)

Correlation of the microculture-kinetic drug-induced apoptosis assay with patient outcomes in initial treatment of adult acute myelocytic leukemia.

Science.gov (United States)

Strickland, Stephen A; Raptis, Anastasios; Hallquist, Allan; Rutledge, James; Chernick, Michael; Perree, Mathieu; Talbott, Mahsa S; Presant, Cary A

2013-03-01

Overall survival (OS) with acute myeloid leukemia (AML) remains poor. Determining prognostic factors will help in selecting patients for appropriate treatments. Our aim was to determine whether the level of drug-induced apoptosis (chemosensitivity) demonstrated by the microculture-kinetic drug-induced apoptosis (MiCK) assay significantly predicted outcomes after standard AML induction therapy. A total of 109 patients with untreated AML had blood and/or bone marrow aspirate samples analyzed for anthracycline-induced apoptosis using the MiCK assay. The amount of apoptosis observed over 48 h was determined and expressed as kinetic units of apoptosis (KU). Complete remission (CR) was significantly higher (72%) in patients with high idarubicin-induced apoptosis >3 KU compared to patients with apoptosis ≤ 3 KU (p = 0.01). Multivariate analysis showed the only significant variables to be idarubicin-induced apoptosis and karyotype. Median overall survival of patients with idarubicin-induced apoptosis >3 KU was 16.1 months compared to 4.5 months in patients with apoptosis ≤ 3 KU (p = 0.004). Multivariate analysis showed the only significant variable to be idarubicin-induced apoptosis. Chemotherapy-induced apoptosis measured by the MiCK assay demonstrated significant correlation with outcomes and appears predictive of complete remission and overall survival for patients receiving standard induction chemotherapy.

ONR Far East Scientific Bulletin, Volume 7, Number 4, October-December 1982,

Science.gov (United States)

1982-12-01

Japan Professor D. Kunii June 3 on Fluidization Department of ChemicalEngineering Faculty of Engineering University of Tokyo 7-3-1, Hongo , Bunkyo-ku...tentative) Conference on Raman Department of Chemistry Spectroscopy Faculty of Science University of Tokyo 7-3-1, Hongo Bunkyo-ku, Tokyo 113 September

WhichCyp

DEFF Research Database (Denmark)

Rostkowski, Michal; Spjuth, Ola; Rydberg, Patrik

2013-01-01

. AVAILABILITY: The WhichCyp server is freely available for use on the web at http://drug.ku.dk/whichcyp, where the WhichCyp Java program and source code is also available for download. CONTACT: pry@sund.ku.dk SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online....

Spin Electronics

Science.gov (United States)

2003-08-01

Site: University of Tokyo Department of Physics Graduate School of Science 7-3-1 Hongo , Bunkyo-ku Tokyo 113-0033, Japan Date April 5, 2001...University of Tokyo Department of Electrical Engineering 7-3-1, Hongo , Bunkyo-ku Tokyo 113, Japan Date: April 5, 2001 WTEC Attendees: D

Development of a Charged-particle Accumulator Using an RF Confinement Method II

Science.gov (United States)

2006-08-24

Ryugo Hayano, Ph.D., Professor Department of Physics, Graduate School of Science University of Tokyo 7-3-1 Hongo , Bunkyo-ku...NAME(S) AND ADDRESS(ES) University of Tokyo,7-3-1 Hongo , Bunkyo-ku,Tokyo 113-0033,Japan,JP,113-0033 8. PERFORMING ORGANIZATION REPORT NUMBER AOARD

Siim Nestor soovitab : Scratch Perverts. Q-Boy. Sümpaatsed väiksed peod / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2005-01-01

Üritustest 11. veebr. Tallinnas: klubis Hip Hop Café diskorid Tony Vegas, Prime Cuts, Plus One, Paul Oja ja Critical; klubis BonBon inglise räppar Q-Boy ja MC Ms Fontaine; klubis Võitlev Sõna üritus "Lift" ja 12. veebr. klubis KuKu üritus "Turbodisko"

Making the Road While Walking It: A Conversation with Richard Simpson

Science.gov (United States)

Zabel, Robert H.; Kaff, Marilyn; Teagarden, James

2016-01-01

Richard Simpson is professor of special education at the University of Kansas (KU). Dr. Simpson's duties at KU have included roles of staff psychologist, teaching associate, assistant professor, project director, associate professor, professor, and chairperson for the Department of Special Education. He has directed numerous University of Kansas…

Domain structure of a NHEJ DNA repair ligase from Mycobacterium tuberculosis.

Science.gov (United States)

Pitcher, Robert S; Tonkin, Louise M; Green, Andrew J; Doherty, Aidan J

2005-08-19

A prokaryotic non-homologous end-joining (NHEJ) system for the repair of DNA double-strand breaks (DSBs), composed of a Ku homodimer (Mt-Ku) and a multidomain multifunctional ATP-dependent DNA ligase (Mt-Lig), has been described recently in Mycobacterium tuberculosis. Mt-Lig exhibits polymerase and nuclease activity in addition to DNA ligation activity. These functions were ascribed to putative polymerase, nuclease and ligase domains that together constitute a monomeric protein. Here, the separate polymerase, nuclease and ligase domains of Mt-Lig were cloned individually, over-expressed and the soluble proteins purified to homogeneity. The polymerase domain demonstrated DNA-dependent RNA primase activity, catalysing the synthesis of unprimed oligoribonucleotides on single-stranded DNA templates. The polymerase domain can also extend DNA in a template-dependent manner. This activity was eliminated when the catalytic aspartate residues were replaced with alanine. The ligase domain catalysed the sealing of nicked double-stranded DNA designed to mimic a DSB, consistent with the role of Mt-Lig in NHEJ. Deletion of the active-site lysine residue prevented the formation of an adenylated ligase complex and consequently thwarted ligation. The nuclease domain did not function independently as a 3'-5' exonuclease. DNA-binding assays revealed that both the polymerase and ligase domains bind DNA in vitro, the latter with considerably higher affinity. Mt-Ku directly stimulated the polymerase and nuclease activities of Mt-Lig. The polymerase domain bound Mt-Ku in vitro, suggesting it may recruit Mt-Lig to Ku-bound DNA in vivo. Consistent with these data, Mt-Ku stimulated the primer extension activity of the polymerase domain, suggestive of a functional interaction relevant to NHEJ-mediated DSB repair processes.

The University of Kansas High-Throughput Screening Laboratory. Part II: enabling collaborative drug-discovery partnerships through cutting-edge screening technology.

Science.gov (United States)

McDonald, Peter R; Roy, Anuradha; Chaguturu, Rathnam

2011-07-01

The University of Kansas High-Throughput Screening (KU HTS) core is a state-of-the-art drug-discovery facility with an entrepreneurial open-service policy, which provides centralized resources supporting public- and private-sector research initiatives. The KU HTS core was established in 2002 at the University of Kansas with support from an NIH grant and the state of Kansas. It collaborates with investigators from national and international academic, nonprofit and pharmaceutical organizations in executing HTS-ready assay development and screening of chemical libraries for target validation, probe selection, hit identification and lead optimization. This is part two of a contribution from the KU HTS laboratory.

Fiscal 2000 achievement report on the research and development of transfer standard gage for oil flowmeter (final assessment); 2000 nendo sekiyu ryuryokei no iten hyojunki no kenkyu kaihatsu seika hokokusho (saishu hyoka hokokusho)

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

In a servo PD (positive displacement) flowmeter for oil, a differential pressure transmitter detects difference in pressure between the PD flowmeter input and output ports and a rotor is driven by a servo motor so that the pressure difference between before and after the PD flowmeter rotor will be zero. The design enables high-accuracy measurement across a wide range of flow rates and is not easily affected by the surrounding physical conditions such as viscosity and density. For the development of such a flowmeter, it is necessary to develop a rotor that rotates smoothly free of locking in a steady and uniform flow, to build a visualized model that enables the analysis of flow inside the flowmeter which in turn will enable the determination of optimum differential pressure detecting ports, and to connect a newly developed servo mechanism to the thus determined ports. As the result of the research, a servo PD flowmeter is developed, which is a 50mm diameter prototype employing the Invoflex tooth form which is the waveform for a rotor suitable for a high performance flowmeter. Placed in a generally used oil flow calibration liquid, which is gasoline, kerosene, or the like, it covers a flow rate range of 1:10 and reads with an accuracy of {+-}0.1%. It remains stable in performance and suffers but a little change with the passage of time. (NEDO)

Quantification of Forecasting and Change-Point Detection Methods for Predictive Maintenance

Science.gov (United States)

2015-08-19

University of Tokyo 7-3-1 Hongo , Bunkyo-ku, Ttokyo 113-8645, Japan 3 Air Force Research Laboratory, 2977 Hobson Way, Bldg 653 Rm 427 Wright...AFB OH 45433-7734 *** University of Tokyo 7-3-1 Hongo , Bunkyo-ku, Tokyo 113-8645, Japan Abstract This work investigates the application of the

Proceedings of 16th Nordic Semiconductor Meeting Held in Laugarvatn, Iceland on 12-15 June 1994

Science.gov (United States)

1995-01-10

7-3-1 Hongo , Bunkyo-ku, Tokyo 113, Japan Received May 4, 1994; accepted June 16, 1994 Abstract described in detail in a previous work [5]. The...Henrik Stubb, Dept. of Physics, KTH Electrum 229, Badenerstr. 569, VTT Electronics, 7-3-1 Hongo , Bunkyo-ku, Dept. of Electronics, CH-8048 Ziirich

Rozbor morfologie nohy u chlapců a dívek ve věku Infans 2 a Juvenis Analysis of morphology of foot in Moravian male and female students in the age Infans 2 and Juvenis

Directory of Open Access Journals (Sweden)

Michaela Peštuková

2005-02-01

Full Text Available U 106 studentů a 162 studentek z Gymnázia ve Šternberku a Střední integrované školy v Ostravě ve věku 12–18 let byl sledován morfologický typ nohy, index nohy pro hodnocení podélné klenby nožní, vyosení palce a malíku, úhel paty a stav svalstva dolních končetin. U chlapců i dívek byla nalezena výrazně vyšší frekvence výskytu nohy egyptské (71,69 % a 70,99 %. Noha kvadratická se vyskytovala u chlapců ve velmi nízkém procentu (2,83 % s určitou individuální asymetrií, u dívek nalezena nebyla. Diagnostika plochonoží metodou Chippauxe–Šmiřáka poskytla velmi příznivé výsledky (4,94–10,38 % výskytu ploché nohy, metoda podle Szritera–Godunova naopak nález podstatně horší (38,2–43,21 % výskytu ploché nohy. S nulovým stavem vyosení palce jsme se setkali u necelé čtvrtiny souborů. U souboru chlapců byla četnost projevu valgozity a varozity palce téměř vyrovnána, věcně převažovalo varózní postavení palce vpravo. U souboru dívek dominovalo signifikantně valgózní postavení. Průměrné hodnoty se pohybovaly v rozmezí 4 až 7 stupňů. Průměrná hodnota úhlu malíku, charakterizující vbočený malík, byla vysoká (17,73 až 21,90 stupně. Průměrný úhel paty byl diagnostikován v rozmezí 15 až 18 stupňů. Při hodnocení vztahů mezi zkrácením svalů dolní končetiny a poruchami funkčního stavu klenby nožní (pouze u mužů jsme nalezli potvrzení statistické významnosti závislosti u vyosení paty a zkrácení m. triceps surae sin. a signifikantní závislost mezi úhlem paty a zkrácením flexorů kolen sin. Morphological foot type, foot index for longitudinal foot vault, misalignment of the big and little toes, heel angle and lower limb muscles condition were monitored in 106 male students and 162 female students from the Gymnasium school in Šternberk and the Integrated secondary school in Ostrava ranging in age from 12 to 18 years. A considerably

Either non-homologous ends joining or homologous recombination is required to repair double-strand breaks in the genome of macrophage-internalized Mycobacterium tuberculosis.

Science.gov (United States)

Brzostek, Anna; Szulc, Izabela; Klink, Magdalena; Brzezinska, Marta; Sulowska, Zofia; Dziadek, Jaroslaw

2014-01-01

The intracellular pathogen Mycobacterium tuberculosis (Mtb) is constantly exposed to a multitude of hostile conditions and is confronted by a variety of potentially DNA-damaging assaults in vivo, primarily from host-generated antimicrobial toxic radicals. Exposure to reactive nitrogen species and/or reactive oxygen species causes different types of DNA damage, including oxidation, depurination, methylation and deamination, that can result in single- or double-strand breaks (DSBs). These breaks affect the integrity of the whole genome and, when left unrepaired, can lead to cell death. Here, we investigated the role of the DSB repair pathways, homologous recombination (HR) and non-homologous ends joining (NHEJ), in the survival of Mtb inside macrophages. To this end, we constructed Mtb strains defective for HR (ΔrecA), NHEJ [Δ(ku,ligD)], or both DSB repair systems [Δ(ku,ligD,recA)]. Experiments using these strains revealed that either HR or NHEJ is sufficient for the survival and propagation of tubercle bacilli inside macrophages. Inhibition of nitric oxide or superoxide anion production with L-NIL or apocynin, respectively, enabled the Δ(ku,ligD,recA) mutant strain lacking both systems to survive intracellularly. Complementation of the Δ(ku,ligD,recA) mutant with an intact recA or ku-ligD rescued the ability of Mtb to propagate inside macrophages.

Either non-homologous ends joining or homologous recombination is required to repair double-strand breaks in the genome of macrophage-internalized Mycobacterium tuberculosis.

Directory of Open Access Journals (Sweden)

Anna Brzostek

Full Text Available The intracellular pathogen Mycobacterium tuberculosis (Mtb is constantly exposed to a multitude of hostile conditions and is confronted by a variety of potentially DNA-damaging assaults in vivo, primarily from host-generated antimicrobial toxic radicals. Exposure to reactive nitrogen species and/or reactive oxygen species causes different types of DNA damage, including oxidation, depurination, methylation and deamination, that can result in single- or double-strand breaks (DSBs. These breaks affect the integrity of the whole genome and, when left unrepaired, can lead to cell death. Here, we investigated the role of the DSB repair pathways, homologous recombination (HR and non-homologous ends joining (NHEJ, in the survival of Mtb inside macrophages. To this end, we constructed Mtb strains defective for HR (ΔrecA, NHEJ [Δ(ku,ligD], or both DSB repair systems [Δ(ku,ligD,recA]. Experiments using these strains revealed that either HR or NHEJ is sufficient for the survival and propagation of tubercle bacilli inside macrophages. Inhibition of nitric oxide or superoxide anion production with L-NIL or apocynin, respectively, enabled the Δ(ku,ligD,recA mutant strain lacking both systems to survive intracellularly. Complementation of the Δ(ku,ligD,recA mutant with an intact recA or ku-ligD rescued the ability of Mtb to propagate inside macrophages.

Up-regulation of DNA-dependent protein kinase correlates with radiation resistance in oral squamous cell carcinoma

International Nuclear Information System (INIS)

Shintani, Satoru; Mihara, Mariko; Li, Chunnan; Nakahara Yuuji; Hino, Satoshi; Nakashiro, Koh-ichi; Hamakawa, Hiroyuki

2003-01-01

DNA-PK is a nuclear protein with serine/threonine kinase activity and forms a complex consisting of the DNA-PKcs and a heterodimer of Ku70 and Ku80 proteins. Recent laboratory experiments have demonstrated that the DNA-PK complex formation is one of the major pathways by which mammalian cells respond to DNA double-strand breaks induced by ionizing radiation. In this study, we evaluated the relationship between expression levels of DNA-PKcs, Ku70 and Ku80 proteins and radiation sensitivity in oral squamous cell carcinoma (OSCC) cell lines and in OSCC patients treated with preoperative radiation therapy. The OSCC cell lines greatly differed in their response to irradiation, as assessed by a standard colony formation assay. However, the expression levels of the DNA-PK complex proteins were all similar, and there was no association between the magnitude of their expression and the tumor radiation sensitivity. Expression of DNA-PK complex proteins increased after radiation treatment, and the increased values correlated with the tumor radiation resistance. Expression of DNA-PKcs and Ku70 after irradiation was increased in the surviving cells of OSCC tissues irradiated preoperatively. These results suggest that up-regulation of DNA-PK complex protein, especially DNA-PKcs, after radiation treatment correlates to radiation resistance. DNA-PKcs might be a molecular target for a novel radiation sensitization therapy of OSCC. (author)

Parenteral formulation of an antileishmanial drug candidate--tackling poor solubility, chemical instability, and polymorphism.

Science.gov (United States)

Kupetz, Eva; Preu, Lutz; Kunick, Conrad; Bunjes, Heike

2013-11-01

The paullon chalcone derivative KuRei300 is active against Leishmania donovani, the protozoans causing visceral leishmaniasis. The aim of this study was the development of a parenteral formulation of the virtually water insoluble compound in order to enable future studies in mice. Mixed lecithin/bile salt micelles, liposomes, supercooled smectic cholesterol myristate nanoparticles, cubic phase nanoparticles and a triglyceride emulsion were screened for their solubilizing properties. Due to the limited available amount of KuRei300 a passive loading approach with pre-formulated carriers that were incubated with drug substance deposited onto the walls of glass vials was used. The loading capacities of the nanocarriers, the influence of the solid state properties of the drug and its deposits on the loading results and chemical stability aspects of KuRei300 were investigated. Employed methods included HPLC, UV spectroscopy, (1)H NMR, XRPD, and DSC. All nanocarriers substantially improved the solubility of KuRei300; the mixed micelles exhibited the highest drug load. Related to the lipid matrix, however, the smectic nanoparticles solubilized the significantly highest amount of drug. Loading from physically altered drug deposits improved the obtainable concentration to the threefold compared with untreated drug powder. Formulations with KuRei300 must be stored excluded from light under a nitrogen atmosphere as the substance is susceptible to photoisomerization and decomposition. Copyright © 2013 Elsevier B.V. All rights reserved.

Achievement report on research and development in the Sunshine Project in fiscal 1977. Hydrogen energy; 1977 nendo seika hokokusho gaiyoshu. Suiso energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1978-04-01

This paper summarizes achievements in the Sunshine Project related to hydrogen energy in fiscal 1977. In the electrolytic process in hydrogen manufacturing technologies, new composite materials are developed in relation with membranes and electrodes as the high temperature and pressure water decomposition method. A bench-scale water decomposition tank using organic polymer ion exchange membranes is fabricated on a trial basis and tested for studying solid electrolyte decomposition method. In hydrogen manufacturing technologies using thermo-chemical process, discussions are being given on cycles of iron systems, iodine systems and hybrid systems (mixture of thermo and photo chemistry and electrochemistry). For hydrogen transporting and storing technologies, metal hydrides most suitable for hydrogen storage are developed, and storage systems are studied. In hydrogen combustion, elucidation is made on fundamental conditions for mixed and single combustion technologies suitable for prevention of reverse ignition and suppression of NOx generation. Studies are also being made on fuel cells using aqueous solution and solid electrolytes. Studies on hydrogen fueled engines are also described. In hydrogen safety assuring technologies, discussions are being given on prevention of explosion disasters, prevention of embrittlement of materials due to hydrogen and criteria for safety assuring technologies. Descriptions are given also on studies on total hydrogen energy systems and hydrogen fueled automobiles. (NEDO)

Fiscal 1998 research report. Construction model project of the human sensory database; 1998 nendo ningen kankaku database kochiku model jigyo seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

This report summarizes the fiscal 1998 research result on construction of the human sensory database. The human sensory database for evaluating working environment was constructed on the basis of the measurement result on human sensory data (stress and fatigue) of 400 examinees at fields (transport field, control room and office) and in a laboratory. By using the newly developed standard measurement protocol for evaluating summer clothing (shirt, slacks and underwear), the database composed of the evaluation experiment results and the comparative experiment results on human physiological and sensory data of aged and young people was constructed. The database is featured by easy retrieval of various information concerned corresponding to requirements of tasks and use purposes. For evaluating the mass data with large time variation read corresponding to use purposes for every scene, the data detection support technique was adopted paying attention to physical and psychological variable phases, and mind and body events. A meaning of reaction and a hint for necessary measures are showed for every phase and event. (NEDO)

Fiscal 1993 report on technological results. R and D on micromachine technology; 1993 nendo micro machine gijutsu no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1994-12-01

Researches on basic element technology of micromachines are conducted with the view of establishing a mechanical system constituted of minute functional elements that perform autonomous operations in a narrow small part of complicated equipment in a power station for example or in a living body. The areas of activity are 1. research on micro actuators and 2. research on basic technology of micromachine; 1 is classified into researches of (1) shape-memory actuator, (2) bending and stretching type actuator, and (3) integrated micro actuator, while 2 is classified into researches of (1) total system for medical diagnosis, (2) micro tactile sensing technology and laser applied diagnosis/treatment technology, and (3) blood pressure/blood circulation sensing technology. In 1-(1), a chemo-mechanical actuator was studied using a shape memory alloy (SMA) and a high polymer gel, and in 1-(2), an SMA and bimetal were employed as a source of the driving force. Further, examination was also made on a fluid driving type actuator. (NEDO)

Achievement report for fiscal 1990 on research and development of photoreactive materials; 1990 nendo hikari hanno zairyo no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1991-03-01

Activities were performed with an objective to establish fundamental technologies related to photoreactive materials capable of ultra-high density and high resolution indication by controlling structures and assemblies of molecules by using light. The activities were taken in the four fields of elucidation of photoreactive mechanisms and fundamental properties, development of the materials, material processing and constituting technologies, and evaluation technologies. Specifically speaking, the activities were developed on the multiplexing technology applicable to ultra-high density recording, sensitivity responsiveness, and material stability in photochromic materials and photochemical hole burning (PHB) materials. For the photochromic materials, development was performed on photochromic materials to form associations, and on a thin film forming technology by means of the organic molecular beam epitaxial process. For the PHB materials, research was implemented on use of the materials at elevated temperatures, development was made on quinone-based materials, research on enhancement of multiplexity, development on porphyrine-based materials, and research on the organizing technology. In the research of the use at elevated temperatures, development was progressed on a material that can retain under the liquefied nitrogen temperature the status of wavelength multiplexed recording that has been formed under the liquefied helium temperature. (NEDO)

Collection of summaries of Sunshine Program achievement reports for fiscal 1982. Hydrogen energy; 1982 nendo sunshine keikaku seika hokokusho gaiyoshu. Suiso energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1983-04-01

The collection includes achievements of research relating to hydrogen energy. In the research on hydrogen production by electrolysis, electrolysis of water using an acid-type solid polymer electrolyte and electrolysis of water using an alkali-type solid polymer electrolyte are taken up. In the research on hydrogen production by thermochemical methods, studies are conducted on the iodine-based cycle, the bromine-based cycle, materials for devices for the iodine-based cycle, and the mixed cycle. Hydrogen production using high-temperature direct thermolysis and solar radiation is also studied. In the research on hydrogen transportation and storage, use of metallic hydrides in these processes are taken up. In the research on the application of hydrogen, techniques of hydrogen combustion and hydrogen-fueled engines are discussed. In the research on hydrogen safety measures, technologies for the prevention of hydrogen explosions and of hydrogen embrittlement of materials in use with hydrogen are studied. In addition, a study is conducted of a hydrogen energy total system, and research and development is carried out for a plant that produces hydrogen by high-temperature high-pressure electrolysis of water. (NEDO)

Achievement report on the development of solar thermal electric power plant technologies. Annex; Taiyonetsu hatsuden plant gijutsu kaihatsu seika hokokusho. Fuzoku shiryo

Energy Technology Data Exchange (ETDEWEB)

NONE

1985-07-01

The two solar thermal electric power pilot plants are of the tower concentration type and the flat/curved surface concentration type. For the first time in the world, they succeeded in operating at a rated output of 1,000kW in August and September, 1981, respectively. Sunshine was inputted at an unstable rate, and the plants were operated under various load patterns. Studies were conducted and an optimum operating technique is established. Since designing, construction, and operation were carried for two types of pilot plants, quantities of useful data were collected through a variety of experiences. Valuable hints and design data were provided for use in the construction of full-scale power plants in the future. Element units developed for the plants were high-reflectance mirrors, high-precision tracking mechanisms, solar heat collectors of the cavity type and paraboloidal type, and molten salt heat accumulators. The tower concentration type plant exhibits a power generation efficiency of 16-17% and an overall plant efficiency of 3.1-4.4%. The maximum overall efficiency a month is 3.9% with the flat/curved surface concentration type plant. (NEDO)

Collection of summaries of Sunshine Program achievement reports for fiscal 1981. Hydrogen energy; 1981 nendo sunshine keikaku seika hokokusho gaiyoshu. Suuiso energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1982-04-01

The collection includes research on hydrogen production through the electrolysis of water using an acid-type solid polymer electrolyte, electrolysis of water using an alkali-type solid polymer electrolyte, thermochemical method using an iodine-based cycle, thermochemical method using a bromine-based cycle, thermochemical method using a mixed cycle, high-temperature direct thermolysis, and the utilization of solar radiation. Furthermore, it includes a study of materials to build a iodine-based cycle apparatus. In a research on the transportation and storage of hydrogen, technologies of hydrogen transportation and storage using metallic hydrides are taken up. In a research on the application of hydrogen, technologies of hydrogen combustion and hydrogen-fueled engines are discussed. In a research on hydrogen safety measures, technologies for the prevention of hydrogen explosion disasters and of hydrogen embrittlement of materials in use with hydrogen are studied. In addition, a study is conducted of a hydrogen energy total system, and research and development is carried out of a plant that produces hydrogen by means of the high-temperature high-pressure electrolysis of water. (NEDO)

Fiscal 2000 achievement report. Research on machine tool not necessitating hydraulic system; 2000 nendo yuatsu resu kosaku kikai no kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

From the viewpoint that the manufacturing process, expendable items, and recycling should all be taken into consideration when machine tool energy consumption is the matter to discuss, it is concluded that the most important policy to follow in the effort to enhance energy conservation is to enable the tool to operate without hydraulic systems. For the realization of a general-purpose machine tool (lathe) to operate free of hydraulic systems, efforts are exerted to develop element technologies, tool rests, tail stocks, and chuck drives usable for the construction of a practical hydraulic system-free machine tool. In fiscal 2000, comprehensive evaluation of experimental machine tools continued, problems to solve for practical application were put together for the fabrication of improved units, and the improved units and an improved control method were integrated into a prototype of practical machine tools. The prototype was exhibited at Japan International Machine Tool Fair (JIMTOF) as a hydraulic system-free NC (numerically controlled) lathe Type LB300, and won a high valuation. The prototype was then tested for basic performance and for possibility of improvement, and problems to be solved before commercialization were isolated. (NEDO)

Non-liberal subjectivities

DEFF Research Database (Denmark)

Sonnichsen, André

2014-01-01

Paper presented at the one-day exploratory workshop on 'Sovereignty and Social Orders in the Postcolony", co-funded by Faculty of Social Sciences, KU, and Danida.......Paper presented at the one-day exploratory workshop on 'Sovereignty and Social Orders in the Postcolony", co-funded by Faculty of Social Sciences, KU, and Danida....

Development and characterization of a novel C-terminal inhibitor of Hsp90 in androgen dependent and independent prostate cancer cells

International Nuclear Information System (INIS)

Eskew, Jeffery D; Rajewski, Roger A; Blagg, Brian SJ; Manjarrez, Jacob R; Matts, Robert L; Holzbeierlein, Jeffrey M; Vielhauer, George A; Sadikot, Takrima; Morales, Pedro; Duren, Alicia; Dunwiddie, Irene; Swink, Megan; Zhang, Xiaoying; Hembruff, Stacey; Donnelly, Alison

2011-01-01

The molecular chaperone, heat shock protein 90 (Hsp90) has been shown to be overexpressed in a number of cancers, including prostate cancer, making it an important target for drug discovery. Unfortunately, results with N-terminal inhibitors from initial clinical trials have been disappointing, as toxicity and resistance resulting from induction of the heat shock response (HSR) has led to both scheduling and administration concerns. Therefore, Hsp90 inhibitors that do not induce the heat shock response represent a promising new direction for the treatment of prostate cancer. Herein, the development of a C-terminal Hsp90 inhibitor, KU174, is described, which demonstrates anti-cancer activity in prostate cancer cells in the absence of a HSR and describe a novel approach to characterize Hsp90 inhibition in cancer cells. PC3-MM2 and LNCaP-LN3 cells were used in both direct and indirect in vitro Hsp90 inhibition assays (DARTS, Surface Plasmon Resonance, co-immunoprecipitation, luciferase, Western blot, anti-proliferative, cytotoxicity and size exclusion chromatography) to characterize the effects of KU174 in prostate cancer cells. Pilot in vivo efficacy studies were also conducted with KU174 in PC3-MM2 xenograft studies. KU174 exhibits robust anti-proliferative and cytotoxic activity along with client protein degradation and disruption of Hsp90 native complexes without induction of a HSR. Furthermore, KU174 demonstrates direct binding to the Hsp90 protein and Hsp90 complexes in cancer cells. In addition, in pilot in-vivo proof-of-concept studies KU174 demonstrates efficacy at 75 mg/kg in a PC3-MM2 rat tumor model. Overall, these findings suggest C-terminal Hsp90 inhibitors have potential as therapeutic agents for the treatment of prostate cancer

Development and characterization of a novel C-terminal inhibitor of Hsp90 in androgen dependent and independent prostate cancer cells

Directory of Open Access Journals (Sweden)

Eskew Jeffery D

2011-10-01

Full Text Available Abstract Background The molecular chaperone, heat shock protein 90 (Hsp90 has been shown to be overexpressed in a number of cancers, including prostate cancer, making it an important target for drug discovery. Unfortunately, results with N-terminal inhibitors from initial clinical trials have been disappointing, as toxicity and resistance resulting from induction of the heat shock response (HSR has led to both scheduling and administration concerns. Therefore, Hsp90 inhibitors that do not induce the heat shock response represent a promising new direction for the treatment of prostate cancer. Herein, the development of a C-terminal Hsp90 inhibitor, KU174, is described, which demonstrates anti-cancer activity in prostate cancer cells in the absence of a HSR and describe a novel approach to characterize Hsp90 inhibition in cancer cells. Methods PC3-MM2 and LNCaP-LN3 cells were used in both direct and indirect in vitro Hsp90 inhibition assays (DARTS, Surface Plasmon Resonance, co-immunoprecipitation, luciferase, Western blot, anti-proliferative, cytotoxicity and size exclusion chromatography to characterize the effects of KU174 in prostate cancer cells. Pilot in vivo efficacy studies were also conducted with KU174 in PC3-MM2 xenograft studies. Results KU174 exhibits robust anti-proliferative and cytotoxic activity along with client protein degradation and disruption of Hsp90 native complexes without induction of a HSR. Furthermore, KU174 demonstrates direct binding to the Hsp90 protein and Hsp90 complexes in cancer cells. In addition, in pilot in-vivo proof-of-concept studies KU174 demonstrates efficacy at 75 mg/kg in a PC3-MM2 rat tumor model. Conclusions Overall, these findings suggest C-terminal Hsp90 inhibitors have potential as therapeutic agents for the treatment of prostate cancer.

Balance og stofskifte

DEFF Research Database (Denmark)

2011-01-01

Udstilling på Medicinsk Museion. Baseret på bevilling fra Assens Fond. Se mere på http://www.museion.ku.dk/whats-on/exhibitions/balance-and-metabolism/......Udstilling på Medicinsk Museion. Baseret på bevilling fra Assens Fond. Se mere på http://www.museion.ku.dk/whats-on/exhibitions/balance-and-metabolism/...

Siim Nestor soovitab : Stereo ÖÖ. Mutant Disco. Beats from the Vault. Turbodisko / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2006-01-01

Oma debüütalbumit "Migration" tutvustav ameerika diskor Alex Attias üritustel "Stereo ÖÖ" 19. mail Tallinnas Von Krahlis. Üritustest "Mutant Disco" 19. mail Tallinnas klubis Privé ja Tartus 20. mail klubis Illusion, "Beats from the Vault" 19. mail Tallinnas klubis Võit ja "Turbodisko" 20. mail Tallinnas klubis KuKu

TDRSS S-shuttle unique receiver equipment

Science.gov (United States)

Weinberg, A.; Schwartz, J. J.; Spearing, R.

1985-01-01

Beginning with STS-9, the Tracking and Date Relay Satellite system (TDRSS) will start providing S- and Ku-band communications and tracking support to the Space Shuttle and its payloads. The most significant element of this support takes place at the TDRSS White Sands Ground Terminal, which processes the Shuttle return link S- and Ku-band signals. While Ku-band hardware available to other TDRSS users is also applied to Ku-Shuttle, stringent S-Shuttle link margins have precluded the application of the standard TDRSS S-band processing equipment to S-Shuttle. It was therfore found necessary to develop a unique S-Shuttle Receiver that embodies state-of-the-art digital technology and processing techniques. This receiver, developed by Motorola, Inc., enhances link margins by 1.5 dB relative to the standard S-band equipment and its bit error rate performance is within a few tenths of a dB of theory. An overview description of the Space Shuttle Receiver Equipment (SSRE) is presented which includes the presentation of block diagrams and salient design features. Selected, measured performance results are also presented.

The Joint Venture Model of Knowledge Utilization: a guide for change in nursing.

Science.gov (United States)

Edgar, Linda; Herbert, Rosemary; Lambert, Sylvie; MacDonald, Jo-Ann; Dubois, Sylvie; Latimer, Margot

2006-05-01

Knowledge utilization (KU) is an essential component of today's nursing practice and healthcare system. Despite advances in knowledge generation, the gap in knowledge transfer from research to practice continues. KU models have moved beyond factors affecting the individual nurse to a broader perspective that includes the practice environment and the socio-political context. This paper proposes one such theoretical model the Joint Venture Model of Knowledge Utilization (JVMKU). Key components of the JVMKU that emerged from an extensive multidisciplinary review of the literature include leadership, emotional intelligence, person, message, empowered workplace and the socio-political environment. The model has a broad and practical application and is not specific to one type of KU or one population. This paper provides a description of the JVMKU, its development and suggested uses at both local and organizational levels. Nurses in both leadership and point-of-care positions will recognize the concepts identified and will be able to apply this model for KU in their own workplace for assessment of areas requiring strengthening and support.

Heat exchanger design

OpenAIRE

Vítek, Tomáš

2017-01-01

Tato bakalářská práce řeší návrh výměníku tepla pro teplovodní kotel se zplyňovací komorou pro předehřev spalovacího vzduchu odpadním teplem spalin. Hodnoty pro výpočet byly experimentálně naměřeny. Práce obsahuje stručný popis trubkového výměníku tepla, stechiometrický vypočet spalování, návrh geometrických rozměrů výměníku, výpočet tlakových ztrát a výpočet výkonu. Její součástí je také výkresová dokumentace navrženého výměníku. This bachelor thesis solves design of a heat exchanger for ...

Development of a Charged-Particle Accumulator Using an RF Confinement Method VI

Science.gov (United States)

2010-08-10

PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) University of Tokyo,7-3-1 Hongo , Bunkyo-ku...Department of Physics 7-3-1 Hongo , Bunkyo-ku, Tokyo 113-0033 - Phone : 03-5841-4235 - Fax : 03-4496-4043 Period of Performance: 2/9/2009 – 3/8/2010

Association of code status discussion with invasive procedures among advanced-stage cancer and noncancer patients

Directory of Open Access Journals (Sweden)

Sasaki A

2017-07-01

Full Text Available Akinori Sasaki,1 Eiji Hiraoka,1 Yosuke Homma,2 Osamu Takahashi,3 Yasuhiro Norisue,4 Koji Kawai,5 Shigeki Fujitani4 1Department of Internal Medicine, 2Department of Emergency Medicine, Tokyo Bay Urayasu Ichikawa Medical Center, Urayasu City, Chiba, 3Department of Internal Medicine, St. Luke’s International Hospital, Chuo-ku, Tokyo, 4Department of Critical Care Medicine, Tokyo Bay Urayasu Ichikawa Medical Center, Urayasu City, Chiba, 5Department of Gastroenterology, Ito Municipal Hospital, Ito City, Shizuoka, Japan Background: Code status discussion is associated with a decrease in invasive procedures among terminally ill cancer patients. We investigated the association between code status discussion on admission and incidence of invasive procedures, cardiopulmonary resuscitation (CPR, and opioid use among inpatients with advanced stages of cancer and noncancer diseases. Methods: We performed a retrospective cohort study in a single center, Ito Municipal Hospital, Japan. Participants were patients who were admitted to the Department of Internal Medicine between October 1, 2013 and August 30, 2015, with advanced-stage cancer and noncancer. We collected demographic data and inquired the presence or absence of code status discussion within 24 hours of admission and whether invasive procedures, including central venous catheter placement, intubation with mechanical ventilation, and CPR for cardiac arrest, and opioid treatment were performed. We investigated the factors associated with CPR events by using multivariate logistic regression analysis. Results: Among the total 232 patients, code status was discussed with 115 patients on admission, of which 114 (99.1% patients had do-not-resuscitate (DNR orders. The code status was not discussed with the remaining 117 patients on admission, of which 69 (59% patients had subsequent code status discussion with resultant DNR orders. Code status discussion on admission decreased the incidence of central venous

[An intergenerational health promotion program involving older adults in urban areas. "Research of Productivity by Intergenerational Sympathy (REPRINTS)": first-year experience and short-term effects].

Science.gov (United States)

Fujiwara, Yoshinori; Nishi, Mariko; Watanabe, Naoki; Lee, Sangyoon; Inoue, Kazuko; Yoshida, Hiroto; Sakuma, Naoko; Kureta, Youichi; Ishii, Kenji; Uchida, Hayato; Kakuno, Fumihiko; Shinkai, Shoji

2006-09-01

We have launched a new intervention study, called "Research of Productivity by Intergenerational Sympathy (REPRINTS)" in which senior volunteers engage in reading picture books to children. The "REPRINTS" program consistently involves social roles and intellectual activity, two higher-level functional capacities. This study reported findings and problems experienced through "REPRINTS" during the first year, ascertained potential effectiveness of social activity, and proposed methods for continued activity. Basic concepts of "REPRINTS"program include "contribution to society", "life-long learning", and "group activity." Sixty seven volunteers and 74 controls, all aged 60 years and over living in three areas, ie., Chuo-ku, central Tokyo, Kawasaki city, suburb of Tokyo and Nagahama city, a local city, participated in a baseline health check-up in June, 2004. After completion of 3-month training seminars (once a week, 2 hr per session), volunteers visited public elementary schools and kindergartens in groups of 6-10 persons for 6 months. They were assessed again by follow-up health check-up in March, 2005. At baseline, the proportion of those who had no grand children (41.8% vs. 20.3%, P= 0.006), average school years (13.4 +/- 2.5 vs. 12.3 +/- 2.5 years, P= 0.008), having any experience of volunteer activities (79.1% vs. 52.7%, P=0.001), and an usual walking speed (86.7 +/- 12.3 vs. 81.3 +/- 12.9 m/min, P=0.012) were significantly higher in volunteers than in controls. There was no significant difference in other baseline characteristics between the two groups. At follow-up, social network scores for 56 volunteers were significantly improved: frequency of contact with grandchildren and others around neighborhood and size of circles of friends and acquaintances were increased, as compared to controls. Social support scores for the volunteers significantly decreased in the receiving aspect, while increased in the giving aspect. In addition, consciousness of loving

Multiple and variable NHEJ-like genes are involved in resistance to DNA damage in Streptomyces ambofaciens

Directory of Open Access Journals (Sweden)

Grégory Hoff

2016-11-01

Full Text Available Non homologous end-joining (NHEJ is a double strand break (DSB repair pathway which does not require any homologous template and can ligate two DNA ends together. The basic bacterial NHEJ machinery involves two partners: the Ku protein, a DNA end binding protein for DSB recognition and the multifunctional LigD protein composed a ligase, a nuclease and a polymerase domain, for end processing and ligation of the broken ends. In silico analyses performed in the 38 sequenced genomes of Streptomyces species revealed the existence of a large panel of NHEJ-like genes. Indeed, ku genes or ligD domain homologues are scattered throughout the genome in multiple copies and can be distinguished in two categories: the core NHEJ gene set constituted of conserved loci and the variable NHEJ gene set constituted of NHEJ-like genes present in only a part of the species. In Streptomyces ambofaciens ATCC 23877, not only the deletion of core genes but also that of variable genes led to an increased sensitivity to DNA damage induced by electron beam irradiation. Multiple mutants of ku, ligase or polymerase encoding genes showed an aggravated phenotype compared to single mutants. Biochemical assays revealed the ability of Ku-like proteins to protect and to stimulate ligation of DNA ends. RT-qPCR and GFP fusion experiments suggested that ku-like genes show a growth phase dependent expression profile consistent with their involvement in DNA repair during spores formation and/or germination.

Výzkum církve a náboženského života v raně novověkých městech

Czech Academy of Sciences Publication Activity Database

Mikulec, Jiří

2013-01-01

Roč. 32, č. 2 (2013), s. 139-151 ISSN 0231-7443. [Města ve středověku a raném novověku jako badatelské téma posledních dvou desetiletí. Praha, 10.10.2011-11.10.2011] Institutional support: RVO:67985963 Keywords : Early Modern period * towns * church * religious life Subject RIV: AB - History

Wpływ terapii BEMER na wybrane wskaźniki krwi w ramach procesu odnowy biologicznej po wysiłku wytrzymałościowym. Studium przypadku = Effect of BEMER therapy on selected indicators of blood in the process of wellness after exercise endurance. Case study

Directory of Open Access Journals (Sweden)

Mirosław Mrozkowiak

2015-04-01

Instytut Kultury Fizycznej, Wydział Kultury Fizycznej, Zdrowia i Turystyki, Uniwersytet Kazimierza Wielkiego, Bydgoszcz, Polska     Słowa kluczowe: terapia BEMER, wskaźniki krwi, odnowa biologiczna, wysiłek wytrzymałościowy, studium przypadku.   Keywords: BEMER therapy, blood indicators, wellness, exercise endurance, case study.   Streszczenie   Zasadniczymi naczyniami tworzącymi mikrokrążenie są naczynia włosowate. Pojedyncze naczynie włosowate ma średnicę około 6 p.m, sumaryczna powierzchnia przekroju naczyń włosowatych wynosi około 3000 cm2. Na każdy milimetr sześcienny ciała ludzkiego przypada średnio 600 naczyń włosowatych. Terapia systemem BEMR przy zastosowaniu kompleksowym lub uzupełniającym doskonali podstawowe procesy fizjologiczne, angiokinezę mikrokrążenia, doprowadzenie składników odżywczych i tlenu do komórek mięśniowych. Dzięki określonej modulacji biorytmicznej uzyskuje się synergiczny wpływ również na pierwotne i wtórne, nieco większe naczynia krwionośne. Materiał i metody. Badany mężczyzna realizujący zdrowy styl życia poddał się dwukrotnie pomiarowi wybranych wskaźników krwi i układu naczyniowego: po wysiłku wytrzymałościowym w czasie restytucji fizjologicznej oraz odnowie wspomaganej terapią BEMER. Uzyskane wyniki. Najbardziej znaczące zmiany w wybranych wskaźnikach krwi po wysiłku wytrzymałościowym zaobserwowano w: całkowitej ilości hemoglobiny najmniejsze w stężeniu jonów wodorowęglowych. Znamienne zmiany wystąpiły w okresie wczesnej odnowy we wskaźnikach: cząstkowym ciśnieniu tlenu (PO2, nasyceniu krwi tętniczej tlenem (SO2 (c, oksyhemoglobinie (O2Hb, karboksyhemoglobinie (HbCO, methemoglobinie (MeHb i hydroksyhemoglobinie (HHb.         Wnioski. Zastosowana metodyka i profil sygnału BEMER wpływa zróżnicowanie na wielkość wybranych cech krwi. Zastosowanie stymulacji BEMER w odnowie biologicznej po wysiłkach wytrzymałościowych u mężczyzn w 5

A Detailed Examination of the GPM Core Satellite Gridded Text Product

Science.gov (United States)

Stocker, Erich Franz; Kelley, Owen A.; Kummerow, C.; Huffman, George; Olson, William S.; Kwiatowski, John M.

2015-01-01

The Global Precipitation Measurement (GPM) mission quarter-degree gridded-text product has a similar file format and a similar purpose as the Tropical Rainfall Measuring Mission (TRMM) 3G68 quarter-degree product. The GPM text-grid format is an hourly summary of surface precipitation retrievals from various GPM instruments and combinations of GPM instruments. The GMI Goddard Profiling (GPROF) retrieval provides the widest swath (800 km) and does the retrieval using the GPM Microwave Imager (GMI). The Ku radar provides the widest radar swath (250 km swath) and also provides continuity with the TRMM Ku Precipitation Radar. GPM's Ku+Ka band matched swath (125 km swath) provides a dual-frequency precipitation retrieval. The "combined" retrieval (125 km swath) provides a multi-instrument precipitation retrieval based on the GMI, the DPR Ku radar, and the DPR Ka radar. While the data are reported in hourly grids, all hours for a day are packaged into a single text file that is g-zipped to reduce file size and to speed up downloading. The data are reported on a 0.25deg x 0.25 deg grid.

Radiation-induced apoptosis of chicken lymphocyte B-cell line DT40

International Nuclear Information System (INIS)

Furusawa, Y.; Aoki, M.; Takakura, K.

2003-01-01

Full text: Ionizing radiation causes lesions of DNA, cell cycle arrest, induced cell death, and apoptosis in the irradiated cells. Then it is easy to expect that those events would be increased in a cell line which is defective in DNA repair system. However, induction of apoptosis by irradiation takes so complicated process when the cells are defective of DNA repair system. Indeed by many recent studies it has been clarified that DNA repair gene is also concerned with apoptotic event and some study shows the contrary data. Thus, the relationship between the genetics of apoptosis and that of DNA repair is still unclear. In this study two kinds of DNA repair proteins, Rad54 and Ku70, were focused. Proteins of Rad54 and Ku70 have important role at two type of DNA repair systems called homologous recombination repair and non-homologous end joining repair, respectively. 4 phenotypes of DT40, parent type, ku70-/-, rad54-/- and ku70-/-/rad54-/- were used to study the radiation-induced apoptosis (Previous study shows that survival fraction of 4 phenotypes of DT40 is decreased in the cell line, in which DNA repair gene is defective). From the results in this study, two things are clarifies. One is that the dependence of apoptotic index on phenotypes is so different between at low dose and at high dose irradiation. The other is that Ku70 has effective role to induce apoptosis in DT40 irradiated with high dose X-rays

Collection of outlines of achievement reports for fiscal 1976 on Sunshine Program. Hydrogen energy; 1976 nendo sunshine keikakaku seika hokokusho gaiyoshu. Suiso energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1977-04-01

Twenty studies are outlined, which are: Hydrogen production technology using electrolysis (Osaka National Research Institute); Hydrogen production technology using high-temperature/high-pressure electrolysis (Showa Denko K.K., and 1 other); Hydrogen production technologies using thermochemical method (4 articles - Osaka National Research Institute; Tokyo National Research Institute; Hitachi, Ltd.; Mitsubishi Heavy Industries, Ltd.); Water decomposition by thermochemical and photochemical hybrid cycle (Yokohama National University); Hydrogen production technology using direct thermolysis (Electrotechnical Laboratory); Hydrogen solidification technology (2 articles - Osaka National Research Institute; Tokyo National Research Institute); Combustion technology (Osaka National Research Institute); Materials for fuel cells (Osaka National Research Institute); Manufacture of fuel cells (Electrotechnical Laboratory); Systematization of fuel cells (Electrotechnical Laboratory); Hydrogen-fueled engine (Mechanical Engineering Laboratory); Disaster prevention technologies for gaseous and liquid hydrogen, etc. (Tokyo National Research Institute); Prevention of embrittlement of materials used with hydrogen (Chugoku National Research Institute); Refining, transportation, and storage systems, and safety techniques for hydrogen (Industrial Research Institute); Hydrogen energy total system (Electrotechnical Laboratory); Comprehensive examination of hydrogen-using subsystems and peripheral technologies (Electrochemical Society of Japan, and 6 others). (NEDO)

FY 1982 annual report on the research and development of automatic sewing systems; 1982 nendo jido hosei system no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1983-03-01

The automatic sewing system technique research association has been commissioned by the Agency of Industrial Science and Technology for (research and development of automatic sewing systems). This program covers R and D of the elementary techniques for total systems and sewing preparation/processing, sewing/assembling, cloth handling, and system management/control. The total system is designed and studied, and automation of the production systems is studied, in order to reduce time for producing unit quantity of diversified types of clothes in small quantities at least by 50% from the current level. The test plant is designed, constructed and operated, to evaluate and confirm its functions for the above purposes. In the FY 1982, the conceptual designs are drawn to establish the overall production systems for producing diversified types of clothes in small quantities. The program for the sewing preparation/processing evaluates various characteristics of cloth to be processed by the sewing system, based on which the cloth characteristics evaluation, cloth stabilization, high-function pattern preparation, and cloth measuring/unfolding/cutting techniques are studied to establish the automatic cloth unfolding/cutting techniques. The FY 1982 efforts are directed to the conceptual designs. (NEDO)

Fiscal 2000 research achievement report. Super-advanced electronics technologies development promotion project; 2000 nendo kenkyu seika hokokusho. Cho sentan gijutsu kaihatsu sokushin jigyo

Energy Technology Data Exchange (ETDEWEB)

NONE

2002-05-01

For the construction of a common base of key technologies in the domain of electronics and information with the influence propagating across a wide range of industries, research and development was conducted of technologies of ultrafine working process, technologies of ultimate measurement, analysis, and control, technologies of new functional electronic materials, etc. Studied in the field of electron beam lithography were high precision and large area imaging technology, highly accurate electron optics control system technology, and beam monitoring technology. Studied in the field of ultrashort wavelength electromagnetic wave patterning system technology were proximity ultrashort wavelength electromagnetic wave patterning system technology, reduced ultrashort wavelength electromagnetic wave patterning system technology, and ultrahigh precision new materials patterning technology. Also studied were technologies of super-advanced plasma measurement, analysis, and control, and technology of super-advanced cleaning. In the field of technologies of new functional elements and films, studies were conducted about technologies of highly sensitive GMR (giant magnetoresistive) film and head fabrication process for contact magnetic recording and technologies of a spin-valve head with submicron track width for contact magnetic recording. (NEDO)

Report on fiscal 1998 results. Item of development, 'research on technologies for disaster prediction'; Saigai yochi gijutsu no kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

Development was conducted, with the results summarized, for an electromagnetic probing system using a superconducting quantum interference device (SQUID) sensor, for the purpose of establishing disaster prediction technologies by grasping the underground structure highly precisely and three-dimensionally. The developed electromagnetic probing system consists of SQUID elements, 3-axial probe with driving circuits, liquid nitrogen Dewars, automatic control circuit of SQUID biases, high speed multi-channel A/D converter, mobile computer and a battery unit. The software has the function of SQUID bias control, measurement, data saving and data analysis. For the electromagnetic probing analytical program, an one- and three-dimensional interpretation programs were developed. As a result of the field tests, the subject system proved to be strong against city noise and advantageous for fault investigation in urban areas. In addition, being a sensor of multi-component/differential measurement, the system is also supposed to be useful in mine surveying. (NEDO)

Fiscal 1998 achievement report. Research and development of advanced clean energy vehicles; 1998 nendo kokoritsu clean energy jidosha no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

The efforts aim to develop advanced clean energy vehicles (ACEVs) which drive on substitutes of oil low in pollution, consuming 1/2 or less energy and emitting 1/2 or less CO2 than the existing vehicles. Studies conducted in fiscal 1998 covered high-efficiency hybrid power systems and ACEVs. Efforts to develop ACEVs involved a reformed methanol fuel cell hybrid passenger car of Nissan Motor Co., Ltd. (improvement on element technologies, study of methanol concentration); CNG (compressed natural gas) engine hybrid passenger car of Honda Research and Development Co., Ltd. (improvement on flywheels, studies of ANG (adsorbent natural gas) adsorbent and ANG tank); CNG ceramics engine hybrid cargo truck of Isuzu Ceramics Research Institute Co., Ltd. (fabrication of ceramics single-cylinder engine, design and fabrication of vehicle control system, fabrication of prototype); CNG lean burn engine hybrid cargo truck of Mitsubishi Motors Co., Ltd. (studies, designing, and fabrication of engine element parts); LNG engine hybrid bus of Nissan Diesel Motor Co., Ltd. (development of engine and power storage); and DME (dimethylether) engine hybrid bus of Hino Motors, Ltd. (development of DME fuel injection system and high-efficiency power storage). (NEDO)

FY 1999 research and development results. Technological development of superconducting power storage systems; Chodendo denryoku chozo system gijutsu kaihatsu 1999 nendo seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

The research and development project is implemented for technological surveys on the superconducting power storage system (SMES) for cost reduction and high-temperature SMES, and the FY 1999 results are reported. The SMES cost analysis/evaluation program establishes the (basic flow for cost analysis/evaluation) for cost evaluation. The program for the SMES systems for system stabilization sets the specifications of 100MW as output and 15kWh as storage capacity at the generator end and intermediate switching station. The program for SMES systems for load fluctuation compensation and frequency control sets the specifications of 100MW as output and 500kWh as storage capacity at the installation site as the load end, and the investigation of high-temperature SMES technology is conducted on the conceptual designs of the SMES for system stabilization application (100MW, 15kWh) of toroidal coil type. The optimization designs are made for these systems. The investigation of the technology for high-temperature superconducting wires involves fabrication on a trial basis and evaluation for the characteristic evaluation coils, and characteristic measurement and applicability investigation for the large-current short conductor. (NEDO)

1998 Annual Study Report. Standardization of manufacturing process systems; 1998 nendo seika hokokusho. Seisan process system no hyojunka (STEP kiban kikaku no kaihatsu)

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

The standards for exchanging and sharing product model data are being internationalized, mainly by ISO-10303 (STEP). The current specifications are not fully developed to include all of the functions needed by the industrial circles, e.g., the functions capable of expressing dynamic product models useful for design alterations and manufacturing processes. In order to solve these problems, and, in particular, to propose the specifications which meet the functions required by the domestic industries, the necessary conditions which the STEP basic specifications should satisfy are investigate to propose the Japan-developed international specifications. One of the major results of the efforts in this year is development of the functional model (I-AAM) which allows to analyze the functions to be proposed for the specifications for the mechanical production processes in the Japanese industries. The necessary data model candidates can be selected by the aid of the model. The efforts will be further continued in and after the next year, based on the results obtained in this year, to eventually develop the specifications which can be registered by NWI. (NEDO)

Fiscal 2000 report on result of the full-length cDNA structure analysis; 2000 nendo kanzen cho cDNA kozo kaiseki seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

This paper explains the results of research on full-length cDNA structure analysis for the period from April, 2000 to March, 2001. The outline of human genome sequence was published in June, 2000. In Japan, human gene analysis was such that, as the basic technology of the bio industry, a millennium project was decided in the budget of fiscal 2000. The full-length cDNA structure analysis is the core of the project. The libraries of cDNA were prepared using full-length and more than 4-5kbp-long cDNAs by oligo-capping method. It began from determining partial sequence data at end cDNA, and then, with new clones selected therefrom, full-length human cDNA sequence data were determined. The partial sequence data determined by fiscal 2000 were 1,035,000 clones while the full-length sequence data were 12,144 clones. The sequence data obtained were analyzed by homology search and translated into amino acid coding sequences, with predictions conducted on protein functions. A clustering method was examined that selects new clones from partial sequences. Database was constructed on gene expression profiles and disease-related gene sequence data. (NEDO)

Achievement report for fiscal 1998 on development of advanced household electric appliance recycling technology; 1998 nendo senshinteki kaden recycle gijutsu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

With an objective to develop a household electric appliance recycling technology, research and development has been made on crushing of compressors, separation of resins and metals, and regenerative utilization of urethane used as the refrigerator heat insulating material. In the compressor crushing technology, development was made on a ring hammer crusher of wood pattern horizontal type. In the research of the resin-metal separation technology, the dry-type separation and material purity were enhanced successfully by means of classification of materials to be crushed according to grain sizes, and classification using an eddy current screening device and a specific gravity screening device. In the research of the urethane regenerative utilization technology, the contained fluorocarbon was decomposed at the rate of 99.999% by modifying and rinsing the pyrolytic gas from the urethane by means of cracking, having achieved to make the material innocuous. The fuel gas thus obtained can be utilized for electric power generation. In addition, a method to granulate the material was established by mixing it with thermo-plastic resins. It was discovered that using the recovered urethane, which is pulverized smaller than 300 {mu} m, can elevate the heat insulation performance when it is used as the vacuum heat insulator, proving its capability of being re-utilized in refrigerators. (NEDO)

Fiscal 1998 R and D report on environment-friendly hydrogen production technology; Kankyo chowagata suiso seizo gijutsu kenkyu kaihatsu 1998 nendo seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

For efficient oil-free environment-friendly production of hydrogen necessary for oil refining process and effective use of CO{sub 2}, this project develops the efficient hydrogen production techniques by using a capacity of microorganisms. On the R and D on improvement of screening and breeding for photosynthetic microorganisms, introduction of different hydrogenases, acquisition of hydrogen uptake negative strains, control of photosynthetic pigment expression, breeding of PHB synthesis negative strains were carried out continuously, and some problems were arranged confirming the contribution to increasing a hydrogen production capacity. On the R and D on large-scale cultivation techniques, engineering data were collected by using a module continuously. Based on the engineering data collected by the module test, the feasibility study was made on a possibility as industrial technology from the viewpoint of a profitability and environmental harmony. Screening of bacterial strains suitable for 4 kinds of wastewater and capable of producing hydrogen under extreme conditions, and basic studies on photoreactor and cultivation technique were conducted by outside research organizations. (NEDO)

Fiscal 1980 Sunshine Project research report. Removal technology of H{sub 2}S; 1980 nendo ryuka suiso jokyo gijutsu no kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1981-03-01

This report summarizes the fiscal 1980 research result on removal process and equipment of H{sub 2}S from condensate and waste gas of turbine inlet steam and turbine outlet condensers of geothermal power plants. In the research on continuous measurement method of H{sub 2}S in geothermal steam, the continuous measurement equipment was completed by improving the heat conduction type steam qualitative dilution equipment developed in last year. The test result of this equipment in Onikobe geothermal power plant gave interesting data showing cyclic fluctuation of H{sub 2}S concentration. In the research on treatment of turbine inlet steam, both physical and chemical treatments were unpractical because of their very expensive costs. In the research on treatment of turbine outlet fluid by selecting RET method for waste gas and diffusion method for condensate, the field test result by using real fluid in geothermal power plants made it possible to select the optimum process according to conditions of turbine outlet fluid, and to estimate removal costs of H{sub 2}S precisely. (NEDO)

FY 1998 report of the results of the R and D of human media; 1998 nendo human media no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

In the light of the trial design/manufacture made in the previous fiscal year, the following were conducted in this R and D: design/manufacture of a system to support the combinedly complete design of designer, contemplation of a system to instantly search the necessary images without keyword input, joint design of urban environment after completion of urban redevelopment by virtual reality technology, design/manufacture of a system by which many people can feel reality, and manufacture of a system to enable evaluation of disaster prevention by a model of urban planning design. Further, carried out were exchanges of technology information and survey on the trend of domestic/overseas technology. In the R and D of the Kansei agent and human media database, pre-prototyping of the integrated system was conducted. By this, an image was materialized for realization of an industrial design support system including sensible information and multimedia contents using database. In the R and D of urban environment human media, the following were conducted: design/evaluation support for design including buildings and the whole urban environment; primary development of individual technology by which many people can virtually experience simultaneously landscape, wind environment, refuge behavior at the time of disaster, etc. (NEDO)

Designating Domestic Terrorist Individuals or Groups

Science.gov (United States)

2010-09-01

These individuals and groups include the Black Panther Party, Weather Underground, Covenant Sword and the Arm of the Lord, Ku Klux Klan, Earth...based and left wing and right wing groups such as the Covenant Sword and the Arm of the Lord, Ku Klux Klan, Black Panther Party, and the Weather...TERMS Watch list, domestic terrorism, validation, designation, domestic terrorism organization, material support, domestic surveillance, SHAC, animal

Mistrz i miasto Mirona Pietrowskiego w ujęciu postkolonialnym

OpenAIRE

Korcz, Karolina

2008-01-01

Recenzja książki: M. Pietrowski, Mistrz i Miasto. Kijowskie konteksty Michaiła Bułhakowa. Tłum. I. Kuźmina, A. Jezierska. Wydawnictwo „Bonami”, Poznań 2004, ss. 319. Review of book: Miron Pietrowski, Mistrz i Miasto. Kijowskie konteksty Michaiła Bułhakowa. Tłum. I. Kuźmina, A. Jezierska. Wydawnictwo „Bonami”, Poznań 2004, pp. 319.

20. X avati arhitektuuri- ja disainigaleriis...

Index Scriptorium Estoniae

2004-01-01

Väljapanek "Vaateaken Eesti ekspositsioonile Veneetsia arhitektuuribiennaalil". Eestit esindab 9. Veneetsia arhitektuuribiennaalil Eesti Arhitektide Liidu projekt "Aeg-maha-arhitektuur", idee autor Kristjan Holm, kaasautorid Liina Jänes ja Pille Epner. Kabli rannast Veneetsiasse teisaldatud puust hobusekujulise välikäimla on loonud Agu Külm ja Jukka-Pekka Jalovaara. Ku-Ku klubis esilinastus Marianne Kõrveri, Rain Tolgi ja Andres Maimiku dokumentaalfilm "Surm Veneetsias"

Artvin yöresinin iklim, toprak yapısı, orman alanları, ağaç serveti ve ormancılık çalışmalarıyla ilgili genel bir değerlendirme

OpenAIRE

Yüksek, Turan; Ölmez, Zafer

2002-01-01

Çalışma konusu olan Artvin, Kuzeydoğu Karadeniz Bölgesinde yer almaktadır. Artvin ormanları ılıman iklim kuşağından, sert-karasal iklim kuşağına kadar farklı kuşaklarda yayılış göstermektedir. Artvin yöresi 390471 hektar orman alanına sahiptir. Orman alanlarının 276883 hektarı (%70.91’i) doğal ormanlar, 113588 hektarı (% 29.09’u) baltalık ormanlardan oluşmaktadır. Toplam arazinin % 54.77’si ormanlarla kaplıdır. Artvin Orman Bölge Müdürlüğü ormanları, Picea ssp., Pinus ssp., Abies ...

Professor

DEFF Research Database (Denmark)

Hansen, Henrik

2014-01-01

DEBAT: Danske bistandskroner skal øge den interregionale handel i Afrika - det skaber vækst i Afrika og indirekte handel med danske virksomheder, mener Henrik Hansen, professor ved Økonomisk Institut på KU.......DEBAT: Danske bistandskroner skal øge den interregionale handel i Afrika - det skaber vækst i Afrika og indirekte handel med danske virksomheder, mener Henrik Hansen, professor ved Økonomisk Institut på KU....

Dual inhibition of mTORC1 and mTORC2 perturbs cytoskeletal organization and impairs endothelial cell elongation.

Science.gov (United States)

Tsuji-Tamura, Kiyomi; Ogawa, Minetaro

2018-02-26

Elongation of endothelial cells is an important process in vascular formation and is expected to be a therapeutic target for inhibiting tumor angiogenesis. We have previously demonstrated that inhibition of mTORC1 and mTORC2 impaired endothelial cell elongation, although the mechanism has not been well defined. In this study, we analyzed the effects of the mTORC1-specific inhibitor everolimus and the mTORC1/mTORC2 dual inhibitor KU0063794 on the cytoskeletal organization and morphology of endothelial cell lines. While both inhibitors equally inhibited cell proliferation, KU0063794 specifically caused abnormal accumulation of F-actin and disordered distribution of microtubules, thereby markedly impairing endothelial cell elongation and tube formation. The effects of KU0063794 were phenocopied by paclitaxel treatment, suggesting that KU0063794 might impair endothelial cell morphology through over-stabilization of microtubules. Although mTORC1 is a key signaling molecule in cell proliferation and has been considered a target for preventing angiogenesis, mTORC1 inhibitors have not been sufficient to suppress angiogenesis. Our results suggest that mTORC1/mTORC2 dual inhibition is more effective for anti-angiogenic therapy, as it impairs not only endothelial cell proliferation, but also endothelial cell elongation. Copyright © 2018 Elsevier Inc. All rights reserved.

The genomes and comparative genomics of Lactobacillus delbrueckii phages.

Science.gov (United States)

Riipinen, Katja-Anneli; Forsman, Päivi; Alatossava, Tapani

2011-07-01

Lactobacillus delbrueckii phages are a great source of genetic diversity. Here, the genome sequences of Lb. delbrueckii phages LL-Ku, c5 and JCL1032 were analyzed in detail, and the genetic diversity of Lb. delbrueckii phages belonging to different taxonomic groups was explored. The lytic isometric group b phages LL-Ku (31,080 bp) and c5 (31,841 bp) showed a minimum nucleotide sequence identity of 90% over about three-fourths of their genomes. The genomic locations of their lysis modules were unique, and the genomes featured several putative overlapping transcription units of genes. LL-Ku and c5 virions displayed peptidoglycan hydrolytic activity associated with a ~36-kDa protein similar in size to the endolysin. Unexpectedly, the 49,433-bp genome of the prolate phage JCL1032 (temperate, group c) revealed a conserved gene order within its structural genes. Lb. delbrueckii phages representing groups a (a phage LL-H), b and c possessed only limited protein sequence homology. Genomic comparison of LL-Ku and c5 suggested that diversification of Lb. delbrueckii phages is mainly due to insertions, deletions and recombination. For the first time, the complete genome sequences of group b and c Lb. delbrueckii phages are reported.

Depression in chronic ketamine users: Sex differences and neural bases.

Science.gov (United States)

Li, Chiang-Shan R; Zhang, Sheng; Hung, Chia-Chun; Chen, Chun-Ming; Duann, Jeng-Ren; Lin, Ching-Po; Lee, Tony Szu-Hsien

2017-11-30

Chronic ketamine use leads to cognitive and affective deficits including depression. Here, we examined sex differences and neural bases of depression in chronic ketamine users. Compared to non-drug using healthy controls (HC), ketamine-using females but not males showed increased depression score as assessed by the Center of Epidemiological Studies Depression Scale (CES-D). We evaluated resting state functional connectivity (rsFC) of the subgenual anterior cingulate cortex (sgACC), a prefrontal structure consistently implicated in the pathogenesis of depression. Compared to HC, ketamine users (KU) did not demonstrate significant changes in sgACC connectivities at a corrected threshold. However, in KU, a linear regression against CES-D score showed less sgACC connectivity to the orbitofrontal cortex (OFC) with increasing depression severity. Examined separately, male and female KU showed higher sgACC connectivity to bilateral superior temporal gyrus and dorsomedial prefrontal cortex (dmPFC), respectively, in correlation with depression. The linear correlation of sgACC-OFC and sgACC-dmPFC connectivity with depression was significantly different in slope between KU and HC. These findings highlighted changes in rsFC of the sgACC as associated with depression and sex differences in these changes in chronic ketamine users. Copyright © 2017 Elsevier B.V. All rights reserved.

A polycomb group protein, PHF1, is involved in the response to DNA double-strand breaks in human cell

Science.gov (United States)

Hong, Zehui; Jiang, Jie; Lan, Li; Nakajima, Satoshi; Kanno, Shin-ichiro; Koseki, Haruhiko; Yasui, Akira

2008-01-01

DNA double-strand breaks (DSBs) represent the most toxic DNA damage arisen from endogenous and exogenous genotoxic stresses and are known to be repaired by either homologous recombination or nonhomologous end-joining processes. Although many proteins have been identified to participate in either of the processes, the whole processes still remain elusive. Polycomb group (PcG) proteins are epigenetic chromatin modifiers involved in gene silencing, cancer development and the maintenance of embryonic and adult stem cells. By screening proteins responding to DNA damage using laser micro-irradiation, we found that PHF1, a human homolog of Drosophila polycomb-like, Pcl, protein, was recruited to DSBs immediately after irradiation and dissociated within 10 min. The accumulation at DSBs is Ku70/Ku80-dependent, and knockdown of PHF1 leads to X-ray sensitivity and increases the frequency of homologous recombination in HeLa cell. We found that PHF1 interacts physically with Ku70/Ku80, suggesting that PHF1 promotes nonhomologous end-joining processes. Furthermore, we found that PHF1 interacts with a number of proteins involved in DNA damage responses, RAD50, SMC1, DHX9 and p53, further suggesting that PHF1, besides the function in PcG, is involved in genome maintenance processes. PMID:18385154

Multitude scaling laws in axisymmetric turbulent wake

Science.gov (United States)

Layek, G. C.; Sunita

2018-03-01

We establish theoretically multitude scaling laws of a self-similar (statistical) axisymmetric turbulent wake. At infinite Reynolds number limit, the flow evolves as general power law and a new exponential law of streamwise distance, consistent with the criterion of equilibrium similarity hypothesis. We found power law scalings for components of the homogeneous dissipation rate (ɛ) obeying the non-Richardson-Kolmogorov cascade as ɛu˜ku3 /2/(l R elm ) , ɛv˜kv3 /2/l , kv˜ku/R el2 m, 0 stress, l is the local length scale, and Rel is the Reynolds number. The Richardson-Kolmogorov cascade corresponds to m = 0. For m ≈ 1, the power law agrees with non-equilibrium scaling laws observed in recent experiments of the axisymmetric wake. On the contrary, the exponential scaling law follows the above dissipation law with different regions of existence for power index m = 3. At finite Reynolds number with kinematic viscosity ν, scalings obey the dissipation laws ɛu ˜ νku/l2 and ɛv ˜ νkv/l2 with kv˜ku/R eln. The value of n is preferably 0 and 2. Different possibilities of scaling laws and symmetry breaking process are discussed at length.

Lactobacillus versmoldensis sp. nov., isolated from raw fermented sausage.

Science.gov (United States)

Kröckel, L; Schillinger, U; Franz, C M A P; Bantleon, A; Ludwig, W

2003-03-01

Lactobacillus versmoldensis sp. nov. (KU-3T) was isolated from raw fermented sausages. The new species was present in high numbers, and frequently dominated the lactic acid bacteria (LAB) populations of the products. 16S rDNA sequence data revealed that the isolates are closely related to the species Lactobacillus kimchii DSM 13961T, Lactobacillus paralimentarius DSM 13238T, Lactobacillus alimentarius DSM 20249T and Lactobacillus farciminis DSM 20184T. DNA-DNA reassociation data, however, clearly distinguished the new isolates from these species; they showed a low degree of DNA relatedness with the type strains of this group of phylogenetically closely related lactobacilli. These results warrant separate species status for strain KU-3T, for which the name Lactobacillus versmoldensis sp. nov. is proposed. The type strain is KU-3T (=DSM 14857T =NCCB 100034T =ATCC BAA-478T).

Integrated source and channel encoded digital communication system design study

Science.gov (United States)

Alem, W. K.; Huth, G. K.; Simon, M. K.

1978-01-01

The particular Ku-band carrier, PN despreading, and symbol synchronization strategies, which were selected for implementation in the Ku-band transponder aboard the orbiter, were assessed and evaluated from a systems performance viewpoint, verifying that system specifications were met. A study was performed of the design and implementation of tracking techniques which are suitable for incorporation into the Orbiter Ku-band communication system. Emphasis was placed on maximizing tracking accuracy and communication system flexibility while minimizing cost, weight, and system complexity of Orbiter and ground systems hardware. The payload communication study assessed the design and performance of the forward link and return link bent-pipe relay modes for attached and detached payloads. As part of this study, a design for a forward link bent-pipe was proposed which employs a residual carrier but which is tracked by the existing Costas loop.

Pre-estimation of loading on water bodies from peat production and new pollution control methods (TuVeKu); Turvetuotannon vesistoekuormituksen ennakointi ja uudet hallintamenetelmaet

Energy Technology Data Exchange (ETDEWEB)

Klove, B.; Saukkoriipi, J.; Tuukkanen, T.; Heiderscheidt, E.; Heikkinen, K.; Marttila, H.; Ihme, R.; Depre, L.; Karppinen, A.

2012-10-15

Approximately 25 million cubic metres of peat are produced in Finland each year. Furthermore 23 million cubic metres of peat is extracted for energy purposes in Finland, which is more than 50% of the world's energy peat production. Peat extraction activities such as drainage and the exposure of peat layers are known to increase the amount of water discharging from the catchment areas and to increase the loading of suspended solids and nutrients, like phosphorus and nitrogen. Hence every effort has to be made to reduce pollution from peat extraction by means of the best viable technologies that take account of the whole life-cycle impact on water bodies. Overland flow areas are considered as one of the best available technologies (BAT) for purifying run-off water. Wetlands that are constructed to already drained mires are also a common and practical method of protecting waters nowadays, as undrained areas of mires close to peat extraction areas are often lacking. Constructed wetlands however are not considered to BAT so far. The chemical purification of runoff waters is considered to one of the best available technologies. However the limiting factor for chemical treatment has often been the high costs of the method. Thus the less expensive small-scale chemical treatment has become a norm in small peat production areas with stringent purification requirements. However, there is still insufficient amount of knowledge regarding how effective drained wetlands and small-scale chemical treatment are in the purification of runoff waters. The 'Pre-estimation of loading on water bodies from peat production and new pollution control methods' project (TuVeKu) had two main objectives: (I) To assess the effect of the local and structural characteristics of the peat production areas and water treatment structures to the detected variations in the purification efficiencies. The statistical analysis is based on the gathered pollution monitoring data and gathered

Untitled

Indian Academy of Sciences (India)

Structure and superconductivity in ternary systems of compounds 299. Erra B2 structure do not have T. above 1:2 K (Ku and Meisner 1981); (iii) for. Ao.67Pt3B the T. are 1-6, 28 and 56K for A = Ca,Sr and Ba (Shelton 1978) and. (iv) AOs B, A = Th, T = 3 K; A = Y, T = 6K (Ku 1980). 3. Ternary carbides. Of the known ternary ...

Leptospira borgpetersenii hybrid leucine-rich repeat protein: Cloning and expression, immunogenic identification and molecular docking evaluation.

Science.gov (United States)

Sritrakul, Tepyuda; Nitipan, Supachai; Wajjwalku, Worawidh; La-Ard, Anchalee; Suphatpahirapol, Chattip; Petkarnjanapong, Wimol; Ongphiphadhanakul, Boonsong; Prapong, Siriwan

2017-11-01

Leptospirosis is an important zoonotic disease, and the major outbreak of this disease in Thailand in 1999 was due largely to the Leptospira borgpetersenii serovar Sejroe. Identification of the leucine-rich repeat (LRR) LBJ_2271 protein containing immunogenic epitopes and the discovery of the LBJ_2271 ortholog in Leptospira serovar Sejroe, KU_Sej_R21_2271, led to further studies of the antigenic immune properties of KU_Sej_LRR_2271. The recombinant hybrid (rh) protein was created and expressed from a hybrid PCR fragment of KU_Sej_R21_2271 fused with DNA encoding the LBJ_2271 signal sequence for targeting protein as a membrane-anchoring protein. The fusion DNA was cloned into pET160/GW/D-TOPO® to form the pET160_hKU_R21_2271 plasmid. The plasmid was used to express the rhKU_Sej_LRR_2271 protein in Escherichia coli BL21 Star™ (DE3). The expressed protein was immunologically detected by Western blotting and immunoreactivity detection with hyperimmune sera, T cell epitope prediction by HLA allele and epitope peptide binding affinity, and potential T cell reactivity analysis. The immunogenic epitopes of the protein were evaluated and verified by HLA allele and epitope peptide complex structure molecular docking. Among fourteen best allele epitopes of this protein, binding affinity values of 12 allele epitopes remained unchanged compared to LBJ_2271. Two epitopes for alleles HLA-A0202 and -A0301 had higher IC 50 values, while T cell reactivity values of these peptides were better than values from LBJ_2271 epitopes. Eight of twelve epitope peptides had positive T-cell reactivity scores. Although the molecular docking of two epitopes, 3FPLLKEFLV11/47FPLLKEFLV55 and 50KLSTVPEGV58, into an HLA-A0202 model revealed a good fit in the docked structures, 50KLSTVPEGV58 and 94KLSTVPEEV102 are still considered as the proteins' best epitopes for allele HLA-A0202. The results of this study showed that rhKU_Sej_LRR_2271 protein contained natural immunological properties that should

Fiscal 1976 Sunshine Project result report. Research on solar energy system (weather survey); 1976 nendo taiyo energy system no kenkyu seika hokokusho. Kisho chosa

Energy Technology Data Exchange (ETDEWEB)

NONE

1977-03-31

This report describes the fiscal 1976 research result on weather survey for solar energy systems. Study was made on preparation of the global solar radiation (GSR) map of Japan. To obtain the estimation equation of GSR based on related weather data, analysis was made on data at A type GSR observation sites and related weather data. As some factors effective for estimating monthly mean GSR, a relative sunshine duration, snowfall index (ratio of days more than 10cm in snowfall) and solar altitude index (sine of solar altitude at culmination hour) were selected. The estimation equation was prepared on the basis of these above factors. GSR was affected by relative sunshine duration exceedingly, snowfall by 12%, and solar altitude difference by 6% within an error of 5%. The monthly and annual GSR maps were prepared every site by the above calculation. The continuous observation results in Kagoshima and Fukuoka by recording direct pyranometers are presented. Scattered solar radiation is defined as the difference between simultaneously measured GSR and direct solar radiation. Weather data preparation in Fukuoka for design of solar cooling/heating and hot water supply systems is also described. (NEDO)

FY 1991 Report on the results of the researches on nonlinear photoelectronic materials; 1991 nendo hisenkei hikari denshi zairyo no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1992-03-01

The nonlinear optics with light-light conversion and electro-optical effects are expected to be the optical materials of the next generation for their functions of, e.g., conversion of the infrared laser beams into visual light, and optical switching. The efforts are directed to development of the procedures for exploring and designing various raw materials, and technologies for synthesizing the raw materials and for producing and evaluating the materials, in order to develop highly nonlinear, high-speed responding materials. Three areas are studied; for the organic materials, the chiral nonlinear effects are demonstrated and the new procedure for determining electrical susceptibility ({gamma}) is developed; for the dispersed materials, highly nonlinear susceptibility of glass dispersed with fine particles of semiconductors, e.g., CuCl, is realized, and possibility of controlled dispersion of fine semiconductor particles in an organic polymer is established; and for development of the superlattice-based materials, the layered structures of thin organic superlattice films are studied, the thin semiconductor films of high purity and little defects are prepared, and development of the three-dimensional structures is started. The individual researches are conducted, 3 for the organic materials, 4 for the dispersed materials, and 2 for the superlattice-based materials. The targets of susceptibility and response are 10{sup -8}esu or more and 10ps or less, respectively. (NEDO)

Fiscal 1975 Sunshine Project. Technology assessment on coal gasification and liquefaction; 1975 nendo sekitan no gas ka ekika gijutsu no technology assessment seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1976-03-31

This research was intended to find the desirable direction of technological development, by discussing the effects of coal gasification/liquefaction technologies from every angle, for the purpose coping with the social demand for steadily and continuously obtaining clean energy. This year, the examination was conducted not only on the energy conversion technologies of coal gasification/liquefaction, but also on the impact generating from the entire supply system of coal energy from coal mine development to mining, transportation, storage and conversion to secondary energy. For this purpose, while the outline was grasped of coal resources in the world as well as each technology for the mining, transportation, storage, electric power generation by gasification, and high calorie gasification, a case study was made on the assumption that electric power generation and high calorie gasification were conducted by importing 1,000 tons of coal from overseas, extracting the impact on the basis of this case study. In addition, examination was made on the position of coal energy in the energy supply of the future, with an impact investigated on condition that about 300 million tons were imported equivalent to roughly 16% of energy supply in the year 2000 or about. (NEDO)

Report on achievements in fiscal 1998. Research and development of a welfare techno-system; 1998 nendo welfare techno system kenkyu kaihatsu (Sapporo) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

The surveys and studies having been performed in the present fiscal year are as follows: (1) in the surveys and studies on self-reliant and at-home care-taking for elderly people living in residential houses, using biological information of snowy and cold districts as the parameters, (a) effect of changing the attitude in two-step ascent and descent motions was studied, (b) stair ascent and descent motions were analyzed, (c) standing-up motion using a sitting position handrail was analyzed, and (d) stride-over motion patterns and hip joint movements were experimented; (2) in the studies of a living environment system to support self-reliant way of living of elderly and physically handicapped people in snowy and cold districts, studies were made on (a) shapes of entrance and exit spaces of a residential house, and features of moving motions of elderly and physically handicapped people, (b) living activity patterns and pleasantness centering around the living section in the house of the at-home elderly people, (c) forms of approach spaces for independent residential houses that consider snow disposal: (3) in development and improvement of care-taking devices with importance placed on physical and psychological adapting function of the users and harmony with the users, studies were given on (a) noise characteristics of rooms in which at-home welfare devices are installed, (b) colors and recognizability of switches on welfare devices for elderly persons, (c) drive experiments of moving devices in winter, and (d) motion simulation of ridable devices; and studies were made on (4) moving and riding welfare device systems for elderly and physically handicapped people in snowy and cold districts, and (5) user interface of motor-driven wheelchairs. (NEDO)

Japan`s Sunshine Project. 1991 annual summary of coal liquefaction and gasification; 1991 nendo sunshine keikaku seika hokokusho gaiyoshu. Sekitan no ekika gas ka

Energy Technology Data Exchange (ETDEWEB)

1992-07-01

Out of the research and development on the 1991 Sunshine Project, the results of coal liquefaction/gasification are reported. The basic research of coal liquefaction/gasification is conducted. The research plan for a 150 ton/day scale pilot plant (PP) is worked out for the development of bituminous coal liquefaction technology by NEDOL process. Data of PSU (Process Support Units) operation, especially, are studied. Concerning the data obtained through dismantling of the 50 ton/day PP in Australia which uses Australian Victoria coal due to completion of its operation and also obtained from its support research, they are reflected in the design of a demonstration plant, and the results are arranged for study. Research and development on refining technology of coal-derived liquid such as Illinois coal liquid and on application technology of its products are made. For the development of coal-use hydrogen production technology, conducted is the research of a high temperature gasification PP by entrained flow bed process which is the core of the coal gasification technology. Elementary study with a 2 ton/day furnace is made for the development of the entrained flow bed coal gasification combined cycle power generation system. Also conducted are PP construction, adjusting operation and the overall research operation.

Report on the results of `the R and D of industrial technology information base arrangement`; `Sangyo gijutsu joho kiban seibi kenkyu kaihatsu` seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

In relation to information processing in the open type research information networking, R and D were conducted to establish basic technologies such as grading-up, acceleration, enhancement of efficiency, diversification of application, promotion of usable data accumulation, etc. As to the R and D of software to promote sharing of research information, software was developed which can easily modify and renew management objects for users at research sites, etc., and the target was almost achieved. Concerning the R and D of information supply technology, an approach was presented whereby clients submit bids for each stream of the data requested and the admission decision is made so as to maximize gain of the session under network capacity constraints. Additionally, the R and D were made of software to construct an international information sharing system, a system for multi-lingual I/O, text manipulation and communication, and software for remote use of network based information for scientific computing. 138 refs., 125 figs., 33 tabs.

FY 1995 report on the results of the R and D of biodegradable plastics; 1995 nendo seibunkaisei plastic kenkyu kaihatsu seika hokokusho. Ippan kokaiyo

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-03-01

For the development of biodegradable plastics, the paper made studies on the following items: 1) development of biodegradable plastics through the cultivation of plastic-like polymer producing micro-organisms; 2) development of biodegradable plastics using polysaccharides; 3) development of biodegradable plastics using molecular design/precise polymerization technology. In 1), P of 4HB fraction 10% was biosynthesized in 5L scale and in one stage by giving to fungus body the {gamma}-butyrolactone which was heated/hydrolyzed. In the study of PHB production by gene recombination of algae, the promoter is improved, and it was confirmed that PHB synthetic genes were surely introduced. PHB could be produced 25% by cultivation of acetic acid. In 2), using alkali protease as enzyme catalyst, sugar ester monomer was synthesized from glucose, sucrose and maltose. In 3), effects were grasped of polymerization conditions of succinic acid/1,4-butane diol/carbonate on the molecular weight and reaction velocity. A test on degradability was conducted by the enzyme method to study the structure of olygomer, in particular. Biodegradation of polyurethane was assessed by soil suspension. (NEDO)

Achievement report for fiscal 1990 on research and development of highly crystalline polymeric materials; 1990 nendo kokesshosei kobunshi zairyo no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1991-03-01

Activities were performed to seek polymeric materials having mechanical strength comparable to metallic materials as a result of giving contrivance on the composition and processing method, and bending strength of 100 GPa or more. The activities were taken in the following five fields: (1) a one-dimensional system material, (2) special melting and forming of a multi-dimensional system, (3) compound forming of multi-dimensional molecules, (4) hybrid cross-linking of cross-linking systems, and (5) high-density cross linking of cross-linking systems. In Item (1), researches were made on optimization of the structures, and elongation and fluid processing of thermotropic liquid crystal polyacrylate, wherein the objective was achieved. In Item (2), the objective was achieved on poly (imide-benzobisthiazole) by monoaxial drawing and lamination thereof. Discussions were given on the liquid crystal polymer as to its correlation between the structure and the magnetic field orientation. In Item (3) discussions were given on the composition and processing method for molecule compounded materials reinforced by aromatic polyamide matrix-polyphenylenebenzothiazole. In Item (4), ionomers were discussed. In Item (5), discussions were given on multi-functional diacetylene system and diacetylene group containing polyimide. (NEDO)

FY 1999 achievement report on the R and D of welfare techno-systems (Kyoto); 1999 nendo welfare techno system kenkyu kaihatsu (Kyoto) seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

The R and D were carried out of various kinds of physically functional substitution equipment which supplements the worsened functional ability of the aged/handicapped. In the research on various prototypes of physically functional substitution equipment, experiments on the use were conducted using Japanese speech recognition devices/programs which are standard products commercially available, so that the aged/handicapped can operate equipment (various devices such as home electric appliances) by speech (words). Further, the specifications were studied for technology to use the Japanese speech recognition technique for physically substitution functions. In the R and D of information infrastructure and various kinds of physically functional substitution equipment, the specifications were established for technology to operate lighting equipment, heater and TV by the Japanese speech, and the experiment was made using the prototype manufactured. As a result, the power source operation by speech of TV/lighting desk lump/electric stove has been finished almost certainly. Further study is needed such as TV channel selection by remote control. The investigation was made of the aid needs for the handicapped. (NEDO)

Achievement report for fiscal 1990 on research and development of electrically conductive polymeric materials; 1990 nendo dodensei kobunshi zairyo no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1991-03-01

It is intended to realize new electrically conductive materials characterized by light weight, corrosion resistance and easy-to-process performance, and electrical and electronic materials having functions different from those of metallic conduction mechanism. Therefore, activities were performed to seek technologies for polymeric materials having conductivity greater than 10{sup 5} S/cm and being stable and easy to process. Activities were taken in the following six fields: (1) new hydrocarbon conjugate polymers, (2) excipient conjugate conductive materials, (3) technologies to form thin films of graphite synthesized at low temperatures, (4) conductive polymers of hetero aromatic system, (5) research and development of conductive materials of the hetero containing system and the {pi} conjugate system, and (6) comprehensive investigative research. In (1), thin films of polyacetylene and polyacene systems were formed, in (2), excipient hydrocarbon conjugate polymers and excipient graphite materials were developed, in (3), a high-accuracy process controlled graphite thin film forming technology was developed, in (4), the conductivity was enhanced by using high-order structural control and molecular design, and stability of the conductive polymers of complex annular conjugate system was enhanced, and in (5), conductive polymers of the hetero containing system and the {pi} conjugate system, and flexible graphite fibers were developed. (NEDO)

FY 1999 report on the results of the technology development of next-generation chemical process; 1999 nendo jisedai kagaku process gijutsu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

The R and D were conducted on the naphtha catalytic cracking, selective oxidation reaction of saturated hydrocarbon, new reaction mechanism utilization process, etc., as next-generation chemical process technology in FY 1999, and the results were summarized. As to the technology of naphtha catalytic cracking, developed was La203/p/ZMS-5 zeolite catalyst which is high in cracking activity and high in light-olefin selectivity. By the FS, it was confirmed that this is more excellent in case of the fixed bed reactor than in the present process. Concerning the technology of selective oxidation reaction of saturated hydrocarbon, the basic information/knowledge were obtained about the construction of catalytic principle toward the high selective catalytic partial oxidation, elucidation of a reaction mechanism in alkane oxidation and design of high functional catalysts, elucidation of a mechanism in butan oxidation, synthesis of alkane selective oxidation catalysts, etc. In relation to the technology of new reaction mechanism utilization process, the following were carried out: study of ethyl benzene dehydrogenation using the membrane reactor trially fabricated in the previous fiscal year, development of high efficiency hydrogen penetration membranes and low temperature high activity catalysts, study of the new process by membrane reactor. (NEDO)

Fiscal 1998 research achievement report. Project for promoting development of super-advanced electronic technology; 1998 nendo chosentan denshi gijutsu kaihatsu sokushin jigyo seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-05-01

For the purpose of establishing super-advanced technologies two generations ahead in the field of electronics and information, research and development was carried out involving ultrafine machining process technology, technologies of ultimate measuring, analysis, and control, and technologies of electronic materials equipped with novel functions. In the study of writing systems in which writing is performed directly by an electron beam, writing technologies using electron beams under single-column and multi-column systems were taken up. In the study of ultrashort wavelength electromagnetic wave patterning systems, studies were made about equimultiple ultrashort wavelength and reduced ultrashort wavelength electromagnetic wave patterning. In the study of ultrahigh precision shielding systems, studies involved high precision in situ measurement and control of writing distortion, and ultrahigh precision and high current density electronic optical technologies. Also carried out were research and development of technologies of super-advanced plasma measurement, analysis, and control, technology of cleaning by ultrafine particle control, technology of ultrahigh sensitivity medium, and technologies of new functional elements and film fabrication. In the overall research and survey, surveys were conducted of the trends of development of technology of semiconductor related lithography. (NEDO)

Welfare of farmed fish - nutrition, breeding and diseases

DEFF Research Database (Denmark)

Buchmann, Kurt

Proceeding on the workshop on Welfare of fish which was organised by the research school SCOFDA at KU-LIFE on April 11 and 12, 2007. Prooceedings comprise a total of 18 abstracts which were edited by Kurt Buchmann.......Proceeding on the workshop on Welfare of fish which was organised by the research school SCOFDA at KU-LIFE on April 11 and 12, 2007. Prooceedings comprise a total of 18 abstracts which were edited by Kurt Buchmann....

Learning and study strategies: a learning analytics approach for feedback

OpenAIRE

De Laet, Tinne; Broos, Tom; Pinxten, Maarten; Vanhoudt, Joke; Verbert, Katrien; Van Soom, Carolien; Langie, Greet

2017-01-01

Due to the open entrance in the Flemish (Belgium) higher education system (any student with a secondary education diploma can enter the program), a substantial part of the first-year students enters without the right qualifications, resulting in an overall drop-out rate is around 40% in the Faculties of Science, Engineering Science, Engineering Technology, and Bio-engineering at the KU Leuven. Therefore, KU Leuven staff heavily invest in heavily invest in advising students before and througho...

"Küsse lat's ..." : [luuletused] / Kauksi Ülle

Index Scriptorium Estoniae

Kauksi Ülle, pseud., 1962-

2002-01-01

Sisu: "Küsse lat's ..." ; "Vanaimä, ma lupa, ku suurõss kasu ..." ; "Mis-ti küsüt, et ..." ; Ussõ pääl üteld ; "Mis' sõs ku olõ 'pihlõ' ma kesk umma mäke ..." "Kos' sa olt? ..." ; "Maarahva miil ..." ; "Havvakirju ragi yts miis ..." ; Hanõ vai luigõ ; Võlg ; Mu viimäne laul sullõ ; "üts korgõ puu pak mullõ paika ...". Eluloolisi andmeid autori kohta lk. 369

Izbor faktora rotacije vektora potiska za balističku raketu sa Lambertovim vođenjem / Choice of the rotational factor of the thrust vector for the ballistic missile with Lambert guidance

Directory of Open Access Journals (Sweden)

Danilo V. Ćuk

2008-04-01

Full Text Available Predmet rada jeste primena Lambertovog vođenja na balističku raketu. Koristeći fundamentalnu jednačinu za upravljački vektor brzine razvijena je formula za određivanje ugla orijentacije vektora potiska u zavisnosti od faktora rotacije njegovog pravca. Izvedena formula zahteva Q-matricu koja povezuje diferencijalnu promenu vektora položaja rakete sa odgovarajućom diferencijalnom promenom korelisane brzine za fiksnu poziciju cilja i konstantno vreme slobodnog leta rakete. Izborom parametra rotacije vektora potiska dobijaju se različiti profili ugaonog položaja rakete i ugla njene brzine u zavisnosti od vremena. Podešavanjem parametra rotacije vektora potiska moguće je ostvariti prekid rada raketnog motora pri nultom napadnom uglu i, na taj način, smanjiti početne poremećaje slobodnog leta rakete. / This paper deals with the application of the Lambert guidance to the ballistic missile. By using the fundamental equation for the velocity-to-be-gained, the demanded command for the thrust angle is developed in terms of the rotational factor of its direction. The developed formula requires the Q-matrix which links a differential change in the missile position vector to the corresponding differential change in the correlated velocity for the fixed target position and the constant time of free flight. The choice of the rotational parameter provides different profiles of the missile attitude and flight path angle with time. By adjusting the rotational parameter for the thrust vector, the cut-off of the rocket engine can occur at zero angle of attack and the initial perturbations during free flight may be thus reduced.

Airborne Radar Observations of Severe Hailstorms: Implications for Future Spaceborne Radar

Science.gov (United States)

Heymsfield, Gerald M.; Tian, Lin; Li, Lihua; McLinden, Matthew; Cervantes, Jaime I.

2013-01-01

A new dual-frequency (Ku and Ka band) nadir-pointing Doppler radar on the high-altitude NASA ER-2 aircraft, called the High-Altitude Imaging Wind and Rain Airborne Profiler (HIWRAP), has collected data over severe thunderstorms in Oklahoma and Kansas during the Midlatitude Continental Convective Clouds Experiment (MC3E). The overarching motivation for this study is to understand the behavior of the dualwavelength airborne radar measurements in a global variety of thunderstorms and how these may relate to future spaceborne-radar measurements. HIWRAP is operated at frequencies that are similar to those of the precipitation radar on the Tropical Rainfall Measuring Mission (Ku band) and the upcoming Global Precipitation Measurement mission satellite's dual-frequency (Ku and Ka bands) precipitation radar. The aircraft measurements of strong hailstorms have been combined with ground-based polarimetric measurements to obtain a better understanding of the response of the Ku- and Ka-band radar to the vertical distribution of the hydrometeors, including hail. Data from two flight lines on 24 May 2011 are presented. Doppler velocities were approx. 39m/s2at 10.7-km altitude from the first flight line early on 24 May, and the lower value of approx. 25m/s on a second flight line later in the day. Vertical motions estimated using a fall speed estimate for large graupel and hail suggested that the first storm had an updraft that possibly exceeded 60m/s for the more intense part of the storm. This large updraft speed along with reports of 5-cm hail at the surface, reflectivities reaching 70 dBZ at S band in the storm cores, and hail signals from polarimetric data provide a highly challenging situation for spaceborne-radar measurements in intense convective systems. The Ku- and Ka-band reflectivities rarely exceed approx. 47 and approx. 37 dBZ, respectively, in these storms.

The Faculties of Pharmacy Schools Should Make an Effort to Network with Community Pharmacies.

Science.gov (United States)

Matsushita, Ryo

2016-01-01

By law, medical faculties are mandated to have a designated partner hospital for the purposes of student practical training. In contrast, pharmacy faculties do not have such a legal requirement for student training in a community pharmacy setting. Nevertheless, there are several public and private universities that do have community pharmacies. However, there is no national university that has established both an educational hospital and a community pharmacy. When Kanazawa University (KU) established a graduate school with a clinical pharmacy course, the faculty of KU deemed it necessary to set up an independent community pharmacy for the purpose of practical training. Thus, in 2003, the Acanthus Pharmacy was set up as the first educational community pharmacy in Japan, managed by a nonprofit organization, with the permission of the Ishikawa Pharmaceutical Association and local community pharmacists. Since that time, Acanthus has managed a clinical pharmacy practice for students from both the undergraduate and graduate schools of KU. From 2006, the undergraduate pharmacy program was changed to a 6-year program, and the Acanthus Pharmacy has continued its roles in educating undergraduate pharmaceutical students, medical students, and as a site of early exposure for KU freshmen. From our experience, it is important to have a real clinical environment available to university pharmacy faculty and students, especially in training for community pharmacy practices.

In vitro studies of cellular response to DNA damage induced by boron neutron capture therapy

International Nuclear Information System (INIS)

Perona, M.; Pontiggia, O.; Carpano, M.; Thomasz, L.; Thorp, S.; Pozzi, E.; Simian, M.; Kahl, S.; Juvenal, G.; Pisarev, M.; Dagrosa, A.

2011-01-01

The aim of these studies was to evaluate the mechanisms of cellular response to DNA damage induced by BNCT. Thyroid carcinoma cells were incubated with 10 BPA or 10 BOPP and irradiated with thermal neutrons. The surviving fraction, the cell cycle distribution and the expression of p53 and Ku70 were analyzed. Different cellular responses were observed for each irradiated group. The decrease of Ku70 in the neutrons +BOPP group could play a role in the increase of sensitization to radiation.

Ultra-compact Ku band rectenna

OpenAIRE

Takacs , Alexandru; Aubert , Hervé; Charlot , Samuel

2015-01-01

International audience; This paper addresses an innovative and ultra-compact rectenna designed for energy harvesting or wireless power transfer applications. The presented rectenna uses a printed cross dipoles antenna array and a rectifier implemented with only one silicon Schottky diode. Experimental results show that 1.15 mW of DC power can be obtained for an optimal load impedance of 500 Ω using a compact rectenna (2.5 cm 2 or 0.6 square wavelength) illuminated by an electric field of 60 V...

Mobile belt conveyor

OpenAIRE

Tenora, Jiří

2017-01-01

Cílem bakalářské práce je návrh pásového dopravníku pro přepravu drobného kameniva, zeminy a drobné stavební sutě s dopravním výkonem 60 t/h, výškovým rozdílem 3,5 m a vzdáleností mezi osami bubnů 8 m. Tato práce obsahuje také popis základních částí pásového dopravníku, funkční výpočty podle normy ČSN ISO 5048, návrh hlavních rozměrů dopravníku a pohonu. Celá práce se skládá z technické zprávy a výkresové dokumentace. The aim of this word is to design belt conveyor to transport tiny aggreg...

Pilsētas sarukšanas ietekme uz Rīgas vēsturiskā centra vidi

OpenAIRE

Ķivule, Astra

2013-01-01

Pasaulē katru gadu ievērojami pieaug pilsētās un mazpilsētās dzīvojošo cilvēku skaits. Vienlaikus Eiropa piedzīvo rūkošo pilsētu fenomenu, ko veicina zemā dzimstība un suburbanizācijas procesi. Pie rūkošām pilsētām pieskaitāma arī Rīga, kas pēdējo 20 gadu laikā ir zaudējusi vairāk nekā 20% iedzīvotāju. Šis process atspoguļojas Rīgas vēsturiskā centra vidē, jo īpaši lielajā neapdzīvoto ēku īpatsvarā. Maģistra darbā apzināts neizmantoto ēku skaits Rīgas vēsturiskajā centrā. Neizmantotās ēkas da...

Yerelden Evrensele Japon Anime ve Manga Sanatı

OpenAIRE

TAŞ ALİCENAP, Çiğdem

2014-01-01

Anime ve manga kültürü Uzakdoğu çizgi roman sanatının yoğun üretimi, tarih, din, kültür ve felsefenin konulara yansıması ve Batı çizgi film ve çizgi romanlarından ayrılan farklı tarzlarıyla araştırılması ve öğrenilmesi gereken değerler içermektedir. Japonya’ da çizgi film sanatının Batı’ daki Disney gibi bir endüstrinin dışında bu denli özgün ve başarılı örnekleri elbette o sanatçıların yetiştiği kültürün, tarihin, sanatın ve felsefi değerlerin olduğu kadar eski...

Substitution of the transmembrane domain of Vpu in simian-human immunodeficiency virus (SHIVKU1bMC33) with that of M2 of influenza A results in a virus that is sensitive to inhibitors of the M2 ion channel and is pathogenic for pig-tailed macaques

International Nuclear Information System (INIS)

Hout, David R.; Gomez, Melissa L.; Pacyniak, Erik; Gomez, Lisa M.; Fegley, Barbara; Mulcahy, Ellyn R.; Hill, M. Sarah; Culley, Nathan; Pinson, David M.; Nothnick, Warren; Powers, Michael F.; Wong, Scott W.; Stephens, Edward B.

2006-01-01

The Vpu protein of human immunodeficiency virus type 1 has been shown to shunt the CD4 receptor molecule to the proteasome for degradation and to enhance virus release from infected cells. The exact mechanism by which the Vpu protein enhances virus release is currently unknown but some investigators have shown that this function is associated with the transmembrane domain and potential ion channel properties. In this study, we determined if the transmembrane domain of Vpu could be functionally substituted with that of the prototypical viroporin, the M2 protein of influenza A virus. We constructed chimeric vpu gene in which the transmembrane domain of Vpu was replaced with that of the M2 protein of influenza. This chimeric vpu gene was substituted for the vpu gene in the genome of a pathogenic simian human immunodeficiency virus, SHIV KU-1bMC33 . The resulting virus, SHIV M2 , synthesized a Vpu protein that had a slightly different M r compared to the parental SHIV KU-1bMC33 , reflecting the different sizes of the two Vpu proteins. The SHIV M2 was shown to replicate with slightly reduced kinetics when compared to the parental SHIV KU-1bMC33 but electron microscopy revealed that the site of maturation was similar to the parental virus SHIV KU1bMC33 . We show that the replication and spread of SHIV M2 could be blocked with the antiviral drug rimantadine, which is known to target the M2 ion channel. Our results indicate a dose dependent inhibition of SHIV M2 with 100 μM rimantadine resulting in a >95% decrease in p27 released into the culture medium. Rimantadine did not affect the replication of the parental SHIV KU-1bMC33 . Examination of SHIV M2 -infected cells treated with 50 μM rimantadine revealed numerous viral particles associated with the cell plasma membrane and within intracytoplasmic vesicles, which is similar to HIV-1 mutants lacking a functional vpu. To determine if SHIV M2 was as pathogenic as the parental SHIV KU-1bMC33 virus, two pig-tailed macaques

Nálezy loštické keramiky ve východních Čechách. Příspěvek ke kontaktům východních Čech a severní Moravy

Czech Academy of Sciences Publication Activity Database

Bláha, R.; Frolík, Jan; Sigl, J.

2003-01-01

Roč. 28, - (2003), s. 525-537 ISSN 0231-5823. [Konference archeologů středověku České republiky a Slovenské republiky s hlavním zaměřením na interetnické vztahy ve středověku a jejich odkaz v hmotné kultuře /34./. Opava, 23.09.2002-27.09.2002] R&D Projects: GA AV ČR KSK8002119 Keywords : Middle Ages * Pottery * Eastern Bohemia Subject RIV: AC - Archeology, Anthropology, Ethnology

Korelace sportovní aktivity a stravovacích návyků u dětí předškolního věku a jejich rodičů The correlation of sports activity and eating habits in preschool children and their parents

Directory of Open Access Journals (Sweden)

Jera Zajec

2006-02-01

Full Text Available Cílem výzkumu bylo analyzovat sportovní aktivitu a stravovací návyky u dětí předškolního věku a jejich rodičů. Zajímala nás korelace mezi stravovacími návyky rodičů a dětí předškolního věku a sportovní aktivitou. Pro dotazování 93 rodičů tří až pětiletých dětí ze tří slovinských školek byl použit dotazník obsahující 32 otázek. Údaje byly zpracovány programem SPSS, který počítá frekvenci a kontingenční tabulky. Zjistili jsme, že výživa většiny dětí předškolního věku je pravidelná a vhodná, přestože některé pětileté dívky a tříletí chlapci mají zvýšený BMI (index tělesné hmotnosti. Stravovací návyky rodičů však nejsou ani zdaleka vzorné – přes 50 % má pouze 2 až 3 jídla denně. Přestože nebyla potvrzena žádná statisticky charakteristická korelace, výsledky ukazují, že děti, jejichž rodiče jsou sportovně aktivní, mají vhodnější výživu než děti, jejichž rodiče sportovně aktivní nejsou. Statisticky charakteristické je, že rodiče, kteří jsou sportovně aktivnější, zapojují své děti do sportu ve větší míře. Většina z nich rovněž tráví aktivně prázdniny. Domníváme se, že v době, kdy ještě podléhají vlivu rodičů a učitelů, by děti měly přijmout pozitivní přístup ke sportovně aktivnímu způsobu života a zdravé výživě, které představují základní podmínku normálního způsobu života bez zbytečných problémů způsobených škodlivými návyky a civilizačními chorobami. The aim of this research was to analyze sports activity and eating habits in preschool children and their parents. We were interested in the correlation between parents' and preschool childrens' eating habits and sports activity. A questionnaire consisting of 32 questions was used to interview 93 parents of three to five year old children from 3 Slovene kindergartens. The data was processed by the SPSS program

The C-terminal region of Rad52 is essential for Rad52 nuclear and nucleolar localization, and accumulation at DNA damage sites immediately after irradiation

Energy Technology Data Exchange (ETDEWEB)

Koike, Manabu, E-mail: m_koike@nirs.go.jp [DNA Repair Gene Res., National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555 (Japan); Yutoku, Yasutomo [DNA Repair Gene Res., National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555 (Japan); Graduate School of Science, Chiba University, Yayoicho, Inage-ku, Chiba 263-8522 (Japan); Koike, Aki [DNA Repair Gene Res., National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555 (Japan)

2013-05-31

Highlights: •Rad52 might play a key role in the repair of DSB immediately after irradiation. •EYFP-Rad52 accumulates rapidly at DSB sites and colocalizes with Ku80. •Accumulation of Rad52 at DSB sites is independent of the core NHEJ factors. •Localization and recruitment of Rad52 to DSB sites are dependent on the Rad52 CTR. •Basic amino acids in Rad52 CTR are highly conserved among vertebrate species. -- Abstract: Rad52 plays essential roles in homologous recombination (HR) and repair of DNA double-strand breaks (DSBs) in Saccharomyces cerevisiae. However, in vertebrates, knockouts of the Rad52 gene show no hypersensitivity to agents that induce DSBs. Rad52 localizes in the nucleus and forms foci at a late stage following irradiation. Ku70 and Ku80, which play an essential role in nonhomologous DNA-end-joining (NHEJ), are essential for the accumulation of other core NHEJ factors, e.g., XRCC4, and a HR-related factor, e.g., BRCA1. Here, we show that the subcellular localization of EYFP-Rad52(1–418) changes dynamically during the cell cycle. In addition, EYFP-Rad52(1–418) accumulates rapidly at microirradiated sites and colocalizes with the DSB sensor protein Ku80. Moreover, the accumulation of EYFP-Rad52(1–418) at DSB sites is independent of the core NHEJ factors, i.e., Ku80 and XRCC4. Furthermore, we observed that EYFP-Rad52(1–418) localizes in nucleoli in CHO-K1 cells and XRCC4-deficient cells, but not in Ku80-deficient cells. We also found that Rad52 nuclear localization, nucleolar localization, and accumulation at DSB sites are dependent on eight amino acids (411–418) at the end of the C-terminal region of Rad52 (Rad52 CTR). Furthermore, basic amino acids on Rad52 CTR are highly conserved among mammalian, avian, and fish homologues, suggesting that Rad52 CTR is important for the regulation and function of Rad52 in vertebrates. These findings also suggest that the mechanism underlying the regulation of subcellular localization of Rad52 is

Differential contribution of two Ppd-1 homoeoalleles to early-flowering phenotype in Nepalese and Japanese varieties of common wheat.

Science.gov (United States)

Nguyen, Anh T; Iehisa, Julio C M; Mizuno, Nobuyuki; Nitta, Miyuki; Nasuda, Shuhei; Takumi, Shigeo

2013-12-01

Wheat landraces carry abundant genetic variation in heading and flowering times. Here, we studied flowering-related traits of two Nepalese varieties, KU-4770 and KU-180 and a Japanese wheat cultivar, Shiroganekomugi (SGK). These three wheat varieties showed similar flowering time in a common garden experiment. In total, five significant quantitative trait loci (QTLs) for three examined traits, the heading, flowering and maturation times, were detected using an F2 population of SGK/KU-4770. The QTLs were found at the Ppd-1 loci on chromosomes 2B and 2D and the 2B QTL was also confirmed in another F2 population of SGK/KU-180. The Ppd-D1 allele from SGK and the Ppd-B1 alleles from the two Nepalese varieties might be causal for early-flowering phenotype. The SGK Ppd-D1 allele contained a 2-kb deletion in the 5' upstream region, indicating a photoperiod-insensitive Ppd-D1a allele. Real-time PCR analysis estimating the Ppd-B1 copy number revealed that the two Nepalese varieties included two intact Ppd-B1 copies, putatively resulting in photoperiod insensitivity and an early-flowering phenotype. The two photoperiod-insensitive Ppd-1 homoeoalleles could independently contribute to segregation of early-flowering individuals in the two F2 populations. Therefore, wheat landraces are genetic resources for discovery of alleles useful for improving wheat heading or flowering times.

Japan`s New Sunshine Project. 1996 Annual Summary of Coal Liquefaction and Gasification; 1996 nendo new sunshine keikaku seika hokokusho gaiyoshu. Sekitan no ekika gasuka

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-11-01

In reference to the results of the research and development under the fiscal 1996 New Sunshine Project, a report was summed up on coal liquefaction and coal gasification. As to the R and D of coal liquefaction technology, researches were conducted on liquefaction characteristics and engineering properties by coal kind, catalysts for coal liquefaction, liquefaction reaction of coal and reformation utilization of the liquefied products, liquefaction reaction mechanism and coking mechanism, solubility of coal in solvent and catalytic reaction mechanism, solvent reaction mechanism by hydrogen donor solvent, etc. Concerning the R and D of coal gasification technology, made were the basic study of eco-technology adaptable gasification technology and the study of coal gasification enhancing technology. Further, as to the development of bituminous coal liquefaction technology, carried out were the study in pilot plants and the support study of pilot plants. Additionally, R and D were done of the basic technology of coal liquefaction such as upgrading technology and environmentally acceptable coal liquefaction technology, and of coal hydrogasification technology. 3 refs., 81 figs., 25 tabs.

Report on 1981 result of Sunshine Project. Research on direct liquefaction reactor of coal; 1981 nendo sekitan no chokusetsu ekika hannoki no kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1981-07-01

This paper explains the results of research on direct liquefaction reaction of coal in fiscal 1981. The direct liquefaction is a reaction of three phases, gas-liquid-solid, under high temperature and high pressure. A tube type was employed in order to increase volume efficiency of the reactor, the continuous phase disperse number was decreased in order to bring it close to plug flow, and the paste Re number was increased to some extent in order to prevent sedimentation of coal particles. Hydrogen was supplied from the tube halfway for the purpose of supplementing the lowering of hydrogen partial pressure with the progress of reaction. A gas-liquid separator was installed so that increase in gas quantity was controlled in the rear stage of the reactor. As for catalysts, iron ore-sulfur system was examined which was promising as a disposable catalyst in place of the conventional iron system. Effect of catalytic addition was clearly recognizable in comparison with non-catalytic, with an exothermic peak observed comparable to the coal hydrogenation reaction of 350-420 degree C. Its catalytic effect was also presumable from the high pressure differential thermal analysis curve. The catalytic mechanism in the case where sulfur is added to hematite and limonite is similar to that of Fe{sub 2}O{sub 3} - sulfur catalyst, with the center of activity supposed to be Fe{sub 1-x}S. The catalytic effect is largely dependent on the particle size. (NEDO)

Fiscal 1974 Sunshine Project result report. Research on solar energy utilization systems (photovoltaic power generation); Taiyo energy riyo system chosa kenkyu seika hokokusho. Taiyoko hatsuden

Energy Technology Data Exchange (ETDEWEB)

NONE

1975-03-01

This report summarizes the research result on the guide for developing solar cells. The report includes (1) standard measurement method for solar cells, (2) system configuration and installation site, (3) optimum design and profitability of solar cells and (4) raw materials and pollution prevention. In the 1st research, study was made on the standard for solar radiation intensity, the definition and measurement methods for spectral profiles, and the measurement conditions for solar cells. In the 2nd research, study was made on various basic data for the scale of photovoltaic power systems. In the 3rd research, since it is necessary to obtain characteristics of solar cells with any profiles of physical constants such as impurity, minority carrier life and mobility by simulation of solar cell actions, research was made on the measurement method of minority carrier lives by using a prepared computer program. Technical and economical comparisons were also made between Si solar cells with various structures. In the 4th research, survey was made on various data for compound semiconductors, and study was made on industrial waste pollution. (NEDO)

FY 1999 report on the results of the investigational study of dioxin emission mechanism/control; 1999 nendo dioxin hassei kiko yokusei chosa kenkyu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

For the purpose of promoting the utilization of energy from waste, studies were conducted on the dioxin emission mechanism and non-Br base fire retardant materials which emit no Br base dioxin, and the FY 1999 results were summarized. In the study, using a small-fluidized bed experimental incinerator, survey was made of effects of organic/inorganic chlorine included in solid general waste on the formation/discharge of dioxins. As a result of the experiment, it was found out that organic chlorine compounds discharge more dioxins than inorganic ones, and that the combustion method in which rapid formation of volatile matter in furnace is controlled to realize the homogeneous mixed state is important for control of dioxins. In the study of non-Br base fire retardant materials, a preliminary experiment was conducted on new fire retardant materials added with inorganic carrier, metal complex, etc. to make the confirmation of fire retardation by liquid phase reaction of polymer in combustion conditions and the study of fire retardation by separating liquid phase reaction, pyrolysis reaction, and gaseous phase reaction. Further, simulation of the combustion of polymer materials was carried out. (NEDO)

FY 2000 report on the results of the development of the next generation chemical process technology; 2000 nendo jisedai kagaku process gijutsu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

The R and D were conducted on the next generation chemical process by which no energy losses are produced and the amount of the waste is made extremely small by the new catalytic reaction, the reaction using next generation separation/reaction membrane, etc., and the FY 2000 results were summed up. As to the development of the selective oxidation technology of saturated hydrocarbon, the basic knowledge/information were obtained on the following: construction of the catalytic principle toward the highly selective catalytic partial oxidation, elucidation of the reaction mechanism in alkane oxidation and design of the high functional catalyst, functional design in butane oxidation, characterization of the alkane oxidation catalyst and the application to the catalytic development, synthesis of the alkane selective oxidation catalyst, etc. Relating to the development of the process technology using the new reaction mechanism, a target value of equilibrium conversion of 10% or more was achieved using membrane reactor in the dehydrogenation of ethyl benzene. Further, as to the high performance selective membrane and low temperature active catalyst that are indispensable to the element technology, the continuous study was made, and at the same time themes in this study were arranged. (NEDO)

Achievement report for fiscal 1998. Research and development of advanced functional materials designing platform; 1998 nendo kokino zairyo sekkei platform no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

Stated in this paper are the reports of the working groups (WGs) for the above-named endeavor and the reports on the achievements of the universities to whom the jobs had been commissioned. WG1 carried out research and development related to the coarse-grained molecular dynamics method. WG2 dealt with mesoscale structures, which are quite larger than atoms, such the phase separated structure of polymer molecules and interfacial structures, worked for the construction of a general-purpose simulator system for reproducing/predicting their dynamic properties, and studied the properties of polymers by use of the said simulator system. WG3 worked on polymeric phenomena large in scale in terms of both space and time. Faculty of Engineering, Yamagata University, constructed a materials physical property database necessary in designing materials by simulation. Institute of Industrial Science, Tokyo University, studied the viscoelastic phase isolation at the quantitative level. Tokyo University's Faculty of Engineering-managed study teams investigated the nano-scale structures and properties of polymer materials. Faculty of Engineering, Tokyo Institute of Technology, performed research and development about the meso-scale behavior of multi-constituent polymers. (NEDO)

Fiscal 1998 'Plant Biotechnology in the 21st Century' workshop report; '21 seiki no shokubutsu biotechnology' workshop 1998 nendo seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

The workshop was opened with the opening remarks by Yamada (President of Nara Institute of Science and Technology (NIST)), the overview of plant biotechnology in the 21st century from academia by Shinmyo (Professor of NIST), and the overview of such technology from Ministry of International Trade and Industry by Katao (Chief of Chemical Industry Division). Lectures and discussions of various topics were conducted for 2 days as follows. The effectiveness of a genomic DNA array method for obtaining the genes for switching genes according to daytime, nighttime, drying, salt, high temperature and low temperature for every plant. Current transfer technology of large DNA fragments into plant cell nuclei and chloroplast. Biological evaluation of the physiological functions and complex stress tolerance capacity transformed by transferring complex stress tolerance genes and useful genes for productivity improvement and value addition. Discussion was also held on the importance of a basic research for biotechnology in the 21st century. (NEDO)

Fiscal 2000 survey report on R and D results of advanced clean energy vehicle; 2000 nendo kokoritsu clean energy jidosha no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

With problems inherent to clean energy vehicles such as cruising distance, fuel supply and fuel consumption, ACEVs (advanced clean energy vehicles) are in demand featuring both low pollution and high efficiency compatibly. This paper explains the fiscal 2000 results of development. The target is, by using oil-alternative fuel, to reduce driving energy consumption and carbon dioxide emission to less than half and to control the life cycle cost (total of manufacturing cost, operating cost, fuel cost, etc.) to not more than twice as much as those of conventional vehicles. As ACEVs, an ANG (adsorbed natural gas) engine and flywheel battery mounted passenger car was selected, as were a CNG ceramics engine and capacitor mounted truck, CNG engine and lithium-ion battery mounted truck, LNG engine and capacitor mounted bus, and a DME engine and capacitor mounted bus. All are hybrid systems with an energy saving device. In the research of synthetic fuels, the results of the studies were summarized including the effect of various synthetic light oils on engine performance, fuel characteristics, effect of PM grain size and the optimum properties. (NEDO)

FY 1998 report on the results of the project on the next generation structure housing development; 1998 nendo jisedai kozo jutaku kaihatsu jigyo seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

The purpose of this project is to develop/verify technology to design/construct a structural body of multiple dwelling house which is highly durable and usable at maximum of the limited space with no projections of poles/beams, and is also to develop/verify a composite system of a structure and each house in which residential space can freely be changed horizontally/vertically in response to the leveling-up of living and changes in life style. The following were conducted: construction of an experimental house group with next generation structure; development of technology of effective space utilization/high durability body construction; development of technology of structural analysis/design systematization of antiseismic/damping system; development of commercialization of technology to heighten frame durability, development of technology to heighten durability of finishing materials of a body; development of low cost construction technology; development of technology of interface between a body and each house; verification of housing performance using a next generation body system; generalization of next generation frame system/house system. To reflect the results of the research quickly, the details of the research are systematized, and a draft is worked out of a guide for constructing the next generation frame system/house system which were arranged between the current architecture related laws and business as a general multiple dwelling house. (NEDO)

FY1998 research report on the R and D on recycling technology. Part 1; 1998 nendo recycle nado kankyo gijutsu kekyu kaihatsu seika hokokusho. 1

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

This project aims to develop recycling technology for reduction of environmental burden caused by waste, and promotion of recycling of wastes. As for advanced recycling technology for PET bottles, the facility was improved for improvement of a facility operability and product quality. Study was made on the effect of a raw bale quality and recycled flake colors on a product quality, the forming test with PS or PET labels, and the concentration and effect of washing liquid circulated in flake washing process. As for recycling technology of hard-to-dispose waste plastics, facility improvement and demonstration test were made for continuous operation of dry-distillation/gasification of shredder dusts and separation of nonferrous metals and glass. Study was also made on pulverizing and recycling technologies of FRP bath tubs, and such pulverization costs were estimated. As for production technology of chemical feed and fuel from wastes, study was made on removal technology of non-flammable substances, development of alkaline additives, reacting condition, development of reactors, and use technology as chemical feed. (NEDO)

FY 1998 report on achievements of the verification survey on a new method for load leveling; Fuka heijunka shinshuho jissho chosa 1998 nendo seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

An electrically double-layered capacitor is drawing attention as a new power storage technology that can contribute to power load leveling. The issue is to make the capacitor more compact by enhancing energy density. Discussions to improve the energy density are being given on a method by which internal resistance combined with that in electronic circuits may be allowed to some extent. In addition, in order to verify feasibility of its practical use, investigations are made on the optimal operation pattern of the system, possible quantity of introduction, effect of the introduction, and future possibility to reduce the cost. As demonstration of the element technology used in capacitor modules, fiscal 1997 fabricated 35 single cells each with a volume of one liter, and investigated the positioning at practical application, case studies, and operation patterns. Based on the achievements in fiscal 1997, fiscal 1998 performed fabrication and performance test on a capacitor module in which multiple number of cells are connected, and trial fabrication of a next generation type high-energy density capacitor. In addition, as a study on a total system, discussions were given on design and cost estimation of a hybrid type capacitor which combines the capacitor with a NAS battery. (NEDO)

Fiscal 1997 report on the results of the verification survey of a new load leveling method; Fuka heijunka shinshuho jissho chosa (1997 nendo seika hokokusho)

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

A study was conducted of a practical applicability of the electric double layer capacitor as a new electric power storage technique. In the study, a comparison was made between the capacitor system and the NAS battery which is most commercialized as one for power storage of all the secondary batteries. The capacitor system is excellent in cycle life, system efficiency, and safety/environment, but in energy density it is approximately a tenth of that of the NAS battery so far. The capacitor system, however, is high in filling rate in modules and enables compact design. As to the 35 fabricated electric double layer capacitor cells, the following tests were carried out: charge and discharge, internal resistance measurement, energy density measurement, characteristics of charge/discharge efficiency, self-discharge characteristics, and characteristics of circumferential temperature. As a result of the tests, the cell with volume energy density of 16.4-16.8 Wh/L was fixed to the module with a filling rate of about 93% and achieved an energy density of 15 Wh/L. 26 refs., 175 figs., 75 tabs.

Reports on 1979 result of Sunshine Project. Research on solar energy system (meteorological investigation); 1979 nendo taiyo energy system no kenkyu seika hokokusho. Kisho chosa

Energy Technology Data Exchange (ETDEWEB)

NONE

1980-03-31

The following were implemented with the purpose of collecting, measuring and putting in order the meteorological data required for the R and D on solar energy technology. (1) Observation of direct solar radiation (Nagoya/Sendai), (2) Meteorological observation for the pilot plant site of 1,000kW solar thermal power generation, (3) Studies on estimation of quantity of direct solar radiation, and (4) Studies on characteristics of quantity of direct solar radiation. In (1), the summary and the results were explained on the continuous observation of the quantity of the direct solar radiation conducted in Nagoya and Sendai using a self-recording actinometer. In (2), meteorological observation was conducted for building lots reclaimed from a salt pan at Nio-cho, Mitoyo county, Kagawa prefecture, a scheduled site for the pilot plant. The items were the quantity of global solar radiation, quantity of sky solar radiation, quantity of direct solar radiation, temperature, wet-bulb temperature, wind direction and wind velocity. In (3), A method was developed for estimating the monthly average quantity of the global solar radiation, normal direct solar radiation, horizontal sky solar radiation at an arbitrary spot. In (4), the characteristics of direct/specified direct solar radiation flux curves were elucidated as the basic data for the technological development of solar energy utilization using a sun follower type heat collecting device, with research done on a method for estimating these curves from other meteorological factors. (NEDO)

Achievement report for fiscal 1997 on research and development of solid electrolyte fuel cells; Kotai denkaishitsugata nenryo denchi no kenkyu kaihatsu 1997 nendo seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-07-01

This paper describes the achievements in fiscal 1997 on research and development of solid electrolyte fuel cells. Fuji Electric has demonstrated possibilities of film type cells of predominantly metallic flat plate supporting type of a large-area cell lamination system, and seal-less stack structure. Sanyo Electric has discussed making thinner the composite cell lamination type anode, optimization in sintering temperature, and sealing materials. The Fine Ceramic Center has performed a 1,000-hour test on an La(Sr) MnO{sub 3}-YSZ electrode, in which A-site defect amount was decreased to 0.1-0.02 to stabilize micro-structure air electrodes for an extended period of time. Fujikura has discussed functional materials for high dispersion and slanting in a fuel electrode Ni/YSZ. Mitsubishi Heavy Industries has fabricated MgO-based fuel electrodes on a trial basis, and performed internal reformation and power generation tests by using full-size stacks. Murata Manufacturing Company has verified long-term power generation properties and stability of a three-layered co-sintered film of flat plate type. Mitsui Shipbuilding has reached a near final conclusion on the basic structure of gas separator cells. The Central Electric Power Research Institute has completed a conceptual design on a 300-MW class composite power generation system in which SOFC and gas turbines are combined. The Electric Power Development Company has discussed problems in SOFC composite power generation development using coal gasified fuel. (NEDO)

Fiscal 1997 achievement report. Research and development of comprehensive basic technology on marine resources; Kaiyo shigen sogo kiban gijutsu no kenkyu kaihatsu sogo seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

The deep-sea manganese nodule mining system in this report collects efficiently and continuously a large quantity of manganese nodules in existence in the 5,000m-deep sea floor. The aim of the project is to develop and build an experimental system for a real mining machine and to perform a comprehensive marine test to find out if such a real machine is technologically and economically feasible. The system under this project is divided into four systems, which are a nodule mining system, nodule lifting system, nodule handling system, and a measurement control system. The nodule mining system travels on the sea bottom efficiently collecting manganese nodules and forwarding them into the nodule lifting system. Only a few systems of this kind have so far been developed, however, and therefore much endeavor needs to be exerted for the development of technologies involved. The nodule lifting system is divided into a pump lift unit, air lift unit, and a nodule lifting pipe. The pump lift unit and air lift unit elevate manganese nodules supplied from the nodule collecting unit to the sea surface. The nodule lifting pipe provides a passage for nodules to run through upward. (NEDO)

Japan`s New Sunshine Project. 1994 annual summary of solar energy R and D program; 1994 nendo new sunshine keikaku. Seika hokokusho gaiyoshu (taiyo energy)

Energy Technology Data Exchange (ETDEWEB)

NONE

1994-12-01

The paper reported the results of fiscal 1994 studies on solar energy in the New Sunshine Project. Relating to the technical development for the practical use of photovoltaic power systems, the development of manufacturing technologies for low-cost substrates and the development of element technology for manufacturing low-cost polycrystalline cells/modules were reported as the development of technology for thin substrate polycrystalline solar cells for practical use. As to the research on fabrication technology for thin film solar cells for practical use, reports were made on the research on low-cost fabrication technology for large-area modules and the technological development for qualitative improvement, etc. In respect to the technological development for super-high efficiency solar cells, reported were the technological development for super-high efficiency single crystalline silicon solar cells and the technological development for crystalline compound solar cells, etc. Concerning the research and development of photovoltaic power systems, reports were on the characterization and control of surface/interface recombination velocity of crystalline silicon thin films and the research on surface passivation for high-efficiency silicon solar cells, etc. In regard to the utilization technology of solar thermal energy, the energy conversion technology using chemical reactions and the development of chemical refrigeration and cold storage systems using solar heat were reported as the research and development of utilization technology of solar thermal systems for industrial use.

FY 1991 Report on the results of the research and development of photo-reactive materials; 1991 nendo hikari hanno zairyo no kenkyu kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1992-03-01

This project is aimed at establishment of the basic technologies for photo-reactive materials capable of, e.g., superhigh-density, superhigh-resolution display using photo-functions to control structures and associated conditions of the molecules. More concretely, the efforts are directed to realization of the technologies for multiplexing, sensitivity-response and material stability, applicable to superhigh-density recording by photochromic and photo-chemical burning (PHB) materials. For the photochromic materials, the long-chain alkyl derivatives of photochromic compounds, e.g., spiropyran and fulgide, are formed into thin films by the LB method, in order to develop organic, superhigh-density recording devices by controlling their associated conditions, and controlling of the photochromism reactions in the films is studied. Those studied for the PHB materials include improvement of heat resistance of the materials, development of quinone-based materials and improvement of their degree of multiplexing, and development of porphyrin-based materials and of technologies for structuring them. The comprehensive survey/research activities include extraction of the problems involved in the themes mainly related to the committee activities, and related technologies. (NEDO)

Fiscal 1976 Sunshine Project result report. Research on solar energy utilization systems (solar furnace); 1976 nendo taiyo energy riyo system chosa kenkyu seika hokokusho. Taiyoro

Energy Technology Data Exchange (ETDEWEB)

NONE

1977-03-01

For solar furnace, study was made on analysis of its use purpose, analysis of the profitability of large furnaces, safety, maintenance, solar radiation and construction site conditions. On production of fused refractories by solar furnace, in particular, giving attention on zircon sand and mullite, their fusion property was clarified, and their fine structure was obtained by rapid heating/cooling. On production of Si by solar furnace, in this fiscal year, in particular, study was made on the 1st step (production of coarse Si from silica rock) and 4th step (deposition of Si by decomposition of SiHCl{sub 3}). On study on high-temperature processing followed by chemical reaction, study was made on production of carborundum by solar furnace, its application to high-temperature gas reaction, and its application to heat storage by high-temperature chemical reaction. In addition, for large solar furnace, study was made on its maintenance, safety, pollution problem, and construction site condition based on estimation of direct solar radiation profile maps. Inlands of Tokai, Kii-Hanto and Seto-Naikai districts were confirmed to be suitable for solar furnaces. (NEDO)

Japan's New Sunshine Project. 1998 annual summary of hydrogen energy R and D; New sunshine keikaku 1998 nendo seika hokokusho gaiyoshu. Suiso energy

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-04-01

Summarized herein are the reports on R and D efforts on hydrogen energy, as part of the FY 1998 New Sunshine Project. For production of hydrogen, characteristics related to transport number were investigated for steam electrolysis at high temperature, in which a sintered ceramic powder was used as the electrolyte and the cell was equipped with platinum electrodes. For utilization of hydrogen, energy conversion techniques were investigated using hydrogen occluding alloys for testing methods for alloy microstructures and hydrogenation characteristics, and preparation of and performance testing methods for the cathodes charged with the aid of hydrogen gas. For analysis/assessment for development of hydrogen-related techniques, the investigated items included water electrolysis with solid polymer electrolytes, hydrogen transport techniques using metal hydrides, hydrogen storing techniques using metal hydrides, hydrogen engines, and techniques for preventing hydrogen embrittlement. Analysis/assessment for development of hydrogen turbines was also investigated as one of the 12 R and D themes reported herein. (NEDO)

Report on the surveys in fiscal 1999. Surveys on practical application of the research achievements; 1999 nendo kenkyu seika no jitsuyoka ni kansuru chosa hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-03-01

A number of technological hurdles exist in the process of practically applying the fundamental researches, wherein risks for private business entities to promote the technological development are not at all small. The present surveys are intended to elucidate the actual status as to how a practical application process for technologies is positioned in the series of research and technological development activity floats by private corporations, and what kinds of problems are present, to serve for discussing the way the future supportive environment improvement should be. The 'practical application processes' in private corporations were put into order and analyzed, using as the base the existing study results relative to technological innovation mechanisms. Investigations ere performed on the existing Japanese research and technological development supporting institutions for the practical application processes employed by the private corporations. The result was used for reference of discussing the problems therein. Furthermore, it is indicated that, in the R and D strategies in the U.S.A., profitability is placed as the largest target by means of strengthening the linkage between research and commercialization. This has resulted in decline in the fundamental researches, for which discussions are developed currently. This paper reports investigations on the actual status of business enterprises in the practical application processes. (NEDO)

Fiscal 1980 Sunshine Project research report. Development of slurry applicable to geothermal environment; 1980 nendo chinetsu kankyoka de shiyo kanona deisui no kaihatsu seika hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1981-03-01

This report summarizes the fiscal 1980 research result on development of slurry applicable to geothermal environment. Study was made on the hydrothermal alteration and rheology of the clay suspension slurry system composed of base mud of sepiolite (thermogel), dehydrator of sodium polyacrylate (SPA), and dispersant GT-8. The study result clarified roughly the action mechanism of such slurry under geothermal environment. Such slurry thus achieved an expected target experimentally through various tests on slurry characteristics at higher temperature, slurry curing and slurry conditioning. Test was made on the lubricity of air system drilling fluid. Simulated field test was made on a preventive effect from lost circulation by using combination of various materials. The preventive effect increased with the kind of additional preventive agents. As for waste sludge treatment technology, the agitating capacity of a mixing tank for polymer flocculant was improved. Study was made on possible continuous treatment of sludge by using the above equipment and a continuous sludge caking equipment, resulting in achievement of an expected target. (NEDO)

FY1998 report on the research on support for practical recycling technologies. Part 1; 1998 nendo recyle gijutsu nado jitsuyoka shien kenkyu seika hokokusho. 1

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

This project aims at development, demonstration and utilization of waste recycling technologies. This report summarizes the fiscal 1998 results. The test/demonstration plant for separation and removal of foreign materials was constructed for effective use of waste building wood, and its optimum operation condition was studied. Although some foreign materials exists, 90% of waste building wood chips were separated and recovered through some improvements for the plant. An advanced separation technology is expected. As for fibering technology, fibers with target compression strength were obtained from cedar chips through a disintegration experiment using a newly developed refiner plate. Although the compression strength of fibers obtained from waste building wood chips was slightly poor, it is attained by increasing the target specific gravity of light- weight wooden materials by nearly 10% from 0.1. As for fiber orientation technology, as the tension of a forming belt was adjusted to achieve sufficient width compression, nearly 90% of the target compression strength was attained. A width compression method remains to be solved. (NEDO)

A Systematic Review of the Physical, Mental, Social, and Economic Problems of Immigrant Women in the Perinatal Period in Japan.

Science.gov (United States)

Kita, Sachiko; Minatani, Mariko; Hikita, Naoko; Matsuzaki, Masayo; Shiraishi, Mie; Haruna, Megumi

2015-12-01

The perinatal mortality of immigrants in Japan is higher than that of Japanese women. However, details of the problems of immigrant perinatal women that contribute to worsening of their health are still unknown. This review describes the physical, psychological, social, and economic problems of immigrant women during the perinatal period in Japan. Medline, CINAHL, PsycINFO, and Igaku-Chuo Zasshi were searched and 36 relevant articles were reviewed. The related descriptions were collected and analyzed by using content analysis. The results showed that immigrant perinatal women in Japan experienced the following problems: language barriers, a problematic relationship with a partner, illegal residency, emotional distress, physical distress, adjustment difficulties, lack of utilization of services, social isolation, lack of support, lack of information, low economic status, unsatisfactory health care, and discrimination. These results indicated that multilingual services, strengthening of social and support networks, and political action are necessary to resolve their problems.

Wybrane zagadnienia dotyczące wpływu wysiłku fizycznego na organizm człowieka=Selected issues concerning the impact of physical exercise on the human organism

Directory of Open Access Journals (Sweden)

Wojciech Wojtasik

2015-10-01

3      Instytut Kultury Fizycznej, Uniwersytet Kazimierza Wielkiego w Bydgoszczy   Słowa kluczowe: aktywność fizyczna, wysiłek fizyczny, geny i aktywności fizyczna Keywords: physical activity, physical effort, genes and physical activity   Cel i zakres pracy Ludzie, już w starożytnej Grecji, przywiązywali dużą wagę do zdrowego trybu życia, którego wyznacznikami są m. in.: siła mięśniowa, koordynacja nerwowo-mięśniowa, wydolność krążeniowo-oddechowa. Wysiłek fizyczny oddziałuje na poszczególne układy organizmu człowieka, w tym szczególnie na układ ruchu, krwionośny i oddechowy, neuro-hormonalny, a przez to na funkcjonowanie wielu narządów wewnętrznych. Od wieków prowadzone są liczne badania mające na celu poznanie zmian zachodzących w organizmie na skutek aktywności fizycznej. Zmiany w funkcjonowaniu organizmu poddanemu określonemu wysiłkowi fizycznemu (zarówno statycznemu jak i dynamicznemu określane są poprzez zastosowanie diagnostyki medycznej, praw fizycznych oraz diagnostyki sportowej. W pracy opisano zmiany, jakie zachodzą w organizmie człowieka w czasie wysiłku fizycznego, w takich układach jak: kostno-stawowy, mięśniowy, krwionośny, czy też oddechowy. Opisano także procesy przystosowawcze organizmu i wykazano korzystne oraz niekorzystne działanie (fizyczne, psychiczne i społeczne aktywności fizycznej na organizm człowieka. Sklasyfikowano również wysiłek fizyczny w zależności od przyjętych obecnie kryteriów. Artykuł stanowi częściowe podsumowanie zainteresowań naukowych autorów w latach 2014-2015.

Acute Normal Tissue Reactions in Head-and-Neck Cancer Patients Treated With IMRT: Influence of Dose and Association With Genetic Polymorphisms in DNA DSB Repair Genes

International Nuclear Information System (INIS)

Werbrouck, Joke; Ruyck, Kim de; Duprez, Frederic; Veldeman, Liv; Claes, Kathleen; Eijkeren, Marc van; Boterberg, Tom; Willems, Petra; Vral, Anne; Neve, Wilfried de; Thierens, Hubert

2009-01-01

Purpose: To investigate the association between dose-related parameters and polymorphisms in DNA DSB repair genes XRCC3 (c.-1843A>G, c.562-14A>G, c.722C>T), Rad51 (c.-3429G>C, c.-3392G>T), Lig4 (c.26C>T, c.1704T>C), Ku70 (c.-1310C>G), and Ku80 (c.2110-2408G>A) and the occurrence of acute reactions after radiotherapy. Materials and Methods: The study population consisted of 88 intensity-modulated radiation therapy (IMRT)-treated head-and-neck cancer patients. Mucositis, dermatitis, and dysphagia were scored using the Common Terminology Criteria (CTC) for Adverse Events v.3.0 scale. The population was divided into a CTC0-2 and CTC3+ group for the analysis of each acute effect. The influence of the dose on critical structures was analyzed using dose-volume histograms. Genotypes were determined by polymerase chain reaction (PCR) combined with restriction fragment length polymorphism or PCR-single base extension assays. Results: The mean dose (D mean ) to the oral cavity and constrictor pharyngeus (PC) muscles was significantly associated with the development of mucositis and dysphagia, respectively. These parameters were considered confounding factors in the radiogenomics analyses. The XRCC3c.722CT/TT and Ku70c.-1310CG/GG genotypes were significantly associated with the development of severe dysphagia (CTC3+). No association was found between the investigated polymorphisms and the development of mucositis or dermatitis. A risk analysis model for severe dysphagia, which was developed based on the XRCC3c.722CT/TT and Ku70c.-1310CG/GG genotypes and the PC dose, showed a sensitivity of 78.6% and a specificity of 77.6%. Conclusions: The XRCC3c.722C>T and Ku70c.-1310C>G polymorphisms as well as the D mean to the PC muscles were highly associated with the development of severe dysphagia after IMRT. The prediction model developed using these parameters showed a high sensitivity and specificity

Concordant and opposite roles of DNA-PK and the "facilitator of chromatin transcription" (FACT in DNA repair, apoptosis and necrosis after cisplatin

Directory of Open Access Journals (Sweden)

Calkins Anne S

2011-06-01

Full Text Available Abstract Background Platinum-containing chemotherapy produces specific DNA damage and is used to treat several human solid tumors. Tumors initially sensitive to platinum-based drugs frequently become resistant. Inhibition of DNA repair is a potential strategy to enhance cisplatin effectiveness. After cisplatin treatment, a balance between repair and apoptosis determines whether cancer cells proliferate or die. DNA-dependent protein kinase (DNA-PK binds to DNA double strand breaks (DSBs through its Ku subunits and initiates non-homologous end joining. Inhibition of DNA-PK sensitizes cancer cells to cisplatin killing. The goal of this study is to elucidate the mechanism underlying the effects of DNA-PK on cisplatin sensitivity. Results Silencing the expression of the catalytic subunit of DNA-PK (DNA-PKcs increased sensitivity to cisplatin and decreased the appearance of γH2AX after cisplatin treatment. We purified DNA-PK by its Ku86 subunit and identified interactors by tandem mass spectrometry before and after cisplatin treatment. The structure specific recognition protein 1 (SSRP1, Spt16 and γH2AX appeared in the Ku86 complex 5 hours after cisplatin treatment. SSRP1 and Spt16 form the facilitator of chromatin transcription (FACT. The cisplatin-induced association of FACT with Ku86 and γH2AX was abrogated by DNase treatment. In living cells, SSRP1 and Ku86 were recruited at sites of DSBs induced by laser beams. Silencing SSRP1 expression increased sensitivity to cisplatin and decreased γH2AX appearance. However, while silencing SSRP1 in cisplatin-treated cells increased both apoptosis and necrosis, DNA-PKcs silencing, in contrast, favored necrosis over apoptosis. Conclusions DNA-PK and FACT both play roles in DNA repair. Therefore both are putative targets for therapeutic inhibition. Since DNA-PK regulates apoptosis, silencing DNA-PKcs redirects cells treated with cisplatin toward necrosis. Silencing FACT however, allows both apoptosis and

Inhibiting Heat-Shock Protein 90 Reverses Sensory Hypoalgesia in Diabetic Mice

Directory of Open Access Journals (Sweden)

Michael J Urban

2010-07-01

Full Text Available Increasing the expression of Hsp70 (heat-shock protein 70 can inhibit sensory neuron degeneration after axotomy. Since the onset of DPN (diabetic peripheral neuropathy is associated with the gradual decline of sensory neuron function, we evaluated whether increasing Hsp70 was sufficient to improve several indices of neuronal function. Hsp90 is the master regulator of the heat-shock response and its inhibition can up-regulate Hsp70. KU-32 (N-{7-[(2R, 3R, 4S, 5R-3, 4-dihydroxy-5-methoxy-6, 6-dimethyl-tetrahydro-2H-pyran-2-yloxy]-8-methyl-2-oxo-2H-chromen-3-yl}acetamide was developed as a novel, novobiocin-based, C-terminal inhibitor of Hsp90 whose ability to increase Hsp70 expression is linked to the presence of an acetamide substitution of the prenylated benzamide moiety of novobiocin. KU-32 protected against glucose-induced death of embryonic DRG (dorsal root ganglia neurons cultured for 3 days in vitro. Similarly, KU-32 significantly decreased neuregulin 1-induced degeneration of myelinated Schwann cell DRG neuron co-cultures prepared from WT (wild-type mice. This protection was lost if the co-cultures were prepared from Hsp70.1 and Hsp70.3 KO (knockout mice. KU-32 is readily bioavailable and was administered once a week for 6 weeks at a dose of 20 mg/kg to WT and Hsp70 KO mice that had been rendered diabetic with streptozotocin for 12 weeks. After 12 weeks of diabetes, both WT and Hsp70 KO mice developed deficits in NCV (nerve conduction velocity and a sensory hypoalgesia. Although KU-32 did not improve glucose levels, HbA1c (glycated haemoglobin or insulin levels, it reversed the NCV and sensory deficits in WT but not Hsp70 KO mice. These studies provide the first evidence that targeting molecular chaperones reverses the sensory hypoalgesia associated with DPN.

Problems of Cooling Sealed Fire Areas in Underground Coal Mines / Vybrané Technologie Ochlazování Prostorově Uzavřených Požářišt̆ V Hlubinných Uhelných Dolech

Directory of Open Access Journals (Sweden)

Magnusková Jana

2011-09-01

Full Text Available V současné hornické praxi patří mezi nejpoužívanější způsoby ochlazování prostorově uzavřených požářišť přirozené ochlazování, ochlazování dusíkem, ochlazování vodou a inertní pěnou a ochlazování recirkulací požárních zplodin. Technologie ochlazování pomocí dusíku představuje dvě varianty - použití kapalného dusíku nebo plynného dusíku v prostoru uzavřených důlních děl. Z technicko-bezpečnostního hlediska je aplikace kapalného dusíku již dnes považována za nevyhovující, naproti tomu aplikace plynného dusíku se jeví jako vysoce bezpečná technologie ochlazování výbuchuvzdorně prostorově uzavřeného požářiště. Další z možností ochlazování požářišť v hlubinných uhelných dolech představuje tzv. přirozené ochlazování, kdy jsou sice ekonomické náklady nulové, ovšem ve srovnání s ochlazovacími procesy vyvolanými aplikací chladících technologií však delší doba potřebná k přirozené refrigeraci požářiště způsobuje finanční ztráty.

Fiscal 1993 investigational report on the strengthening of the resource/energy industrial basis of CIS Central Asia (the Republic of Kazakhstan); 1993 nendo CIS Chuo Asia (Kazafusutan kyowakoku) no shigen energy sangyo kiban kyoka ni kansuru chosa hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1994-05-01

The paper grasps the present situation of economy and industry in Kazakhstan and studies a policy for developing the industrial machine manufacturing industry of the country. Since the independence in 1992, the country is now at the great turning point in politics, economy, society and industry. The country is forced to convert from the industrial structure of one local area where much of its own discretion had not been given under the economy system of power centralization to the industrial structure which meets the international economic market system as an independent country. Making good use of agricultural and industrial property in the former U.S.S.R., the country needs to export energy resource like oil, natural gas and coal, and abundant ore resource like iron and non-ferrous metal, which were added with values. As the technical base for it, fostering and strengthening of the industrial machine manufacturing industry is important. At present, the situation is that because of the economic sluggishness, the country cannot manufacture domestically even basic mechanical parts and simply-manufactured parts which are required by the domestic energy industry, machine manufacturing industry, and other industries. The technical program to be proposed by Japan is planned to be worked out later based on the basic research made in fiscal 1993. 6 figs., 14 tabs.

Mezivýměník tepla primárního okruhu reaktoru ESFR

OpenAIRE

Švihel, Miroslav

2012-01-01

Práce je zaměřena především na návrh mezivýměníku tepla primárního okruhu reaktoru ESFR. Výměník je počítán tepelně, hydraulicky i pevnostně a závěrem je zpracována část výkresové dokumentace. Jsou navrhnuty základní rozměry trubkového svazku a nádoby výměníku. Je zde obsažen i přehled koncepcí výměníků a doposud použitých typů mezivýměníku na jaderných elektrárnách s rychlými reaktory. Uvedeny jsou zde i základní parametry projektu ESFR a zhodnocena bezpečnost a provozní spolehlivost výměník...

RPA facilitates telomerase activity at chromosome ends in budding and fission yeasts.

Science.gov (United States)

Luciano, Pierre; Coulon, Stéphane; Faure, Virginie; Corda, Yves; Bos, Julia; Brill, Steven J; Gilson, Eric; Simon, Marie-Noelle; Géli, Vincent

2012-04-18

In Saccharomyces cerevisiae, the telomerase complex binds to chromosome ends and is activated in late S-phase through a process coupled to the progression of the replication fork. Here, we show that the single-stranded DNA-binding protein RPA (replication protein A) binds to the two daughter telomeres during telomere replication but only its binding to the leading-strand telomere depends on the Mre11/Rad50/Xrs2 (MRX) complex. We further demonstrate that RPA specifically co-precipitates with yKu, Cdc13 and telomerase. The interaction of RPA with telomerase appears to be mediated by both yKu and the telomerase subunit Est1. Moreover, a mutation in Rfa1 that affects both the interaction with yKu and telomerase reduces the dramatic increase in telomere length of a rif1Δ, rif2Δ double mutant. Finally, we show that the RPA/telomerase association and function are conserved in Schizosaccharomyces pombe. Our results indicate that in both yeasts, RPA directly facilitates telomerase activity at chromosome ends.

Belt conveyor

OpenAIRE

Hoferek, Tomáš

2016-01-01

Tato bakalářská práce se zabývá problematikou pásového dopravníku pro přepravu suchého písku. Cílem práce je provést konstrukční řešení pásového dopravníku podle zadaných parametrů, kterými jsou dopravní výkon 100 000 kg.h-1, výškový rozdíl 5 m a vzdálenost mezi osami bubnů 40 m. Práce obsahuje krátký popis pásového dopravníku a jeho hlavních částí, funkční výpočet dle platné normy ČSN ISO 5048 a volbu jednotlivých komponentů z firemních katalogů. Dále také návrh napínacího zařízení, návrh po...

Probability of major depression diagnostic classification using semi-structured versus fully structured diagnostic interviews.

Science.gov (United States)

Levis, Brooke; Benedetti, Andrea; Riehm, Kira E; Saadat, Nazanin; Levis, Alexander W; Azar, Marleine; Rice, Danielle B; Chiovitti, Matthew J; Sanchez, Tatiana A; Cuijpers, Pim; Gilbody, Simon; Ioannidis, John P A; Kloda, Lorie A; McMillan, Dean; Patten, Scott B; Shrier, Ian; Steele, Russell J; Ziegelstein, Roy C; Akena, Dickens H; Arroll, Bruce; Ayalon, Liat; Baradaran, Hamid R; Baron, Murray; Beraldi, Anna; Bombardier, Charles H; Butterworth, Peter; Carter, Gregory; Chagas, Marcos H; Chan, Juliana C N; Cholera, Rushina; Chowdhary, Neerja; Clover, Kerrie; Conwell, Yeates; de Man-van Ginkel, Janneke M; Delgadillo, Jaime; Fann, Jesse R; Fischer, Felix H; Fischler, Benjamin; Fung, Daniel; Gelaye, Bizu; Goodyear-Smith, Felicity; Greeno, Catherine G; Hall, Brian J; Hambridge, John; Harrison, Patricia A; Hegerl, Ulrich; Hides, Leanne; Hobfoll, Stevan E; Hudson, Marie; Hyphantis, Thomas; Inagaki, Masatoshi; Ismail, Khalida; Jetté, Nathalie; Khamseh, Mohammad E; Kiely, Kim M; Lamers, Femke; Liu, Shen-Ing; Lotrakul, Manote; Loureiro, Sonia R; Löwe, Bernd; Marsh, Laura; McGuire, Anthony; Mohd Sidik, Sherina; Munhoz, Tiago N; Muramatsu, Kumiko; Osório, Flávia L; Patel, Vikram; Pence, Brian W; Persoons, Philippe; Picardi, Angelo; Rooney, Alasdair G; Santos, Iná S; Shaaban, Juwita; Sidebottom, Abbey; Simning, Adam; Stafford, Lesley; Sung, Sharon; Tan, Pei Lin Lynnette; Turner, Alyna; van der Feltz-Cornelis, Christina M; van Weert, Henk C; Vöhringer, Paul A; White, Jennifer; Whooley, Mary A; Winkley, Kirsty; Yamada, Mitsuhiko; Zhang, Yuying; Thombs, Brett D

2018-06-01

original sponsor of the development of the PHQ-9, which is now in the public domain. Dr Chan is a steering committee member or consultant of Astra Zeneca, Bayer, Lilly, MSD and Pfizer. She has received sponsorships and honorarium for giving lectures and providing consultancy and her affiliated institution has received research grants from these companies. Dr Hegerl declares that within the past 3 years, he was an advisory board member for Lundbeck, Servier and Otsuka Pharma; a consultant for Bayer Pharma; and a speaker for Medice Arzneimittel, Novartis, and Roche Pharma, all outside the submitted work. Dr Inagaki declares that he has received grants from Novartis Pharma, lecture fees from Pfizer, Mochida, Shionogi, Sumitomo Dainippon Pharma, Daiichi-Sankyo, Meiji Seika and Takeda, and royalties from Nippon Hyoron Sha, Nanzando, Seiwa Shoten, Igaku-shoin and Technomics, all outside of the submitted work. Dr Yamada reports personal fees from Meiji Seika Pharma Co., Ltd., MSD K.K., Asahi Kasei Pharma Corporation, Seishin Shobo, Seiwa Shoten Co., Ltd., Igaku-shoin Ltd., Chugai Igakusha and Sentan Igakusha, all outside the submitted work. All other authors declare no competing interests. No funder had any role in the design and conduct of the study; collection, management, analysis and interpretation of the data; preparation, review or approval of the manuscript; and decision to submit the manuscript for publication.

Partitioning of U, Th and K Between Metal, Sulfide and Silicate, Insights into the Volatile-Content of Mercury

Science.gov (United States)

Habermann, M.; Boujibar, A.; Righter, K.; Danielson, L.; Rapp, J.; Righter, M.; Pando, K.; Ross, D. K.; Andreasen, R.; Chidester, B.

2016-01-01

During the early stages of the Solar System formation, especially during the T-Tauri phase, the Sun emitted strong solar winds, which are thought to have expelled a portion of the volatile elements from the inner solar system. It is therefore usually believed that the volatile depletion of a planet is correlated with its proximity to the Sun. This trend was supported by the K/Th and K/U ratios of Venus, the Earth, and Mars. Prior to the MESSENGER mission, it was expected that Mercury is the most volatile-depleted planet. However, the Gamma Ray Spectrometer of MESSENGER spacecraft revealed elevated K/U and K/Th ratios for the surface of Mercury, much higher than previous expectations. It is possible that the K/Th and K/U ratios on the surface are not a reliable gauge of the bulk volatile content of Mercury. Mercury is enriched in sulfur and is the most reduced of the terrestrial planets, with oxygen fugacity (fO2) between IW-6.3 and IW-2.6 log units. At these particular compositions, U, Th and K behave differently and can become more siderophile or chalcophile. If significant amounts of U and Th are sequestered in the core, the apparent K/U and K/Th ratios measured on the surface may not represent the volatile budget of the whole planet. An accurate determination of the partitioning of these elements between silicate, metal, and sulfide phases under Mercurian conditions is therefore essential to better constrain Mercury's volatile content and assess planetary formation models.

Simplification of intradermal skin testing in Hymenoptera venom allergic children.

Science.gov (United States)

Cichocka-Jarosz, Ewa; Stobiecki, Marcin; Brzyski, Piotr; Rogatko, Iwona; Nittner-Marszalska, Marita; Sztefko, Krystyna; Czarnobilska, Ewa; Lis, Grzegorz; Nowak-Węgrzyn, Anna

2017-03-01

The direct comparison between children and adults with Hymenoptera venom anaphylaxis (HVA) has never been extensively reported. Severe HVA with IgE-documented mechanism is the recommendation for venom immunotherapy, regardless of age. To determine the differences in the basic diagnostic profile between children and adults with severe HVA and its practical implications. We reviewed the medical records of 91 children and 121 adults. Bee venom allergy was exposure dependent, regardless of age (P bee venom allergic group, specific IgE levels were significantly higher in children (29.5 kU A /L; interquartile range, 11.30-66.30 kU A /L) compared with adults (5.10 kU A /L; interquartile range, 2.03-8.30 kU A /L) (P venom were higher in bee venom allergic children compared with the wasp venom allergic children (P venom. At concentrations lower than 0.1 μg/mL, 16% of wasp venom allergic children and 39% of bee venom allergic children had positive intradermal test results. The median tryptase level was significantly higher in adults than in children for the entire study group (P = .002), as well as in bee (P = .002) and wasp venom allergic groups (P = .049). The basic diagnostic profile in severe HVA reactors is age dependent. Lower skin test reactivity to culprit venom in children may have practical application in starting the intradermal test procedure with higher venom concentrations. Copyright © 2016 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

Characterisation of manganese peroxidase and laccase producing bacteria capable for degradation of sucrose glutamic acid-Maillard reaction products at different nutritional and environmental conditions.

Science.gov (United States)

Kumar, Vineet; Chandra, Ram

2018-02-02

Maillard reactions products (MRPs) are a major colorant of distillery effluent. It is major source of environmental pollution due to its complex structure and recalcitrant nature. This study has revealed that sucrose glutamic acid-Maillard reaction products (SGA-MRPs) showed many absorption peaks between 200 and 450 nm. The absorption maximum peak was noted at 250 nm in spectrophotometric detection. This indicated the formation of variable molecular weight Maillard products during the SGA-MRPs formation at high temperature. The identified aerobic bacterial consortium consisting Klebsiella pneumoniae (KU726953), Salmonella enterica (KU726954), Enterobacter aerogenes (KU726955), Enterobacter cloaceae (KU726957) showed optimum production of MnP and laccase at 120 and 144 h of growth, respectively. The potential bacterial consortium showed decolourisation of Maillard product up to 70% in presence of glucose (1%), peptone (0.1%) at optimum pH (8.1), temperature (37 °C) and shaking speed (180 rpm) within 192 h of incubation. The reduction of colour of Maillard product correlated with shifting of absorption peaks in UV-Vis spectrophotometry analysis. Further, the changing of functional group in FT-IR data showed appearance of new peaks and GC-MS analysis of degraded sample revealed the depolymerisation of complex MRPs. The toxicity evaluation using seed of Phaseolus mungo L. showed reduction of toxicity of MRPs after bacterial treatment. Hence, this study concluded that developed bacterial consortium have capability for decolourisation of MRPs due to high content of MnP and laccase.

Topologie vnějšku a vnitřku v autorském herectví

Czech Academy of Sciences Publication Activity Database

Koubová, Alice

2013-01-01

Roč. 68, Suppl. 1 (2013), s. 133-143 ISSN 0046-385X R&D Projects: GA ČR(CZ) GAP401/10/1164 Grant - others:AV ČR(CZ) M300091203 Institutional support: RVO:67985955 Keywords : topology * phenomenology * phenopraxis * interiority * exteriority * (inter)acting with the inner partner * authorial acting Subject RIV: AA - Philosophy ; Religion

Neotectonics of central Japan and Izu-Bonin arc. Chuo Nippon no neotectonics to Izu Ogasawara ko

Energy Technology Data Exchange (ETDEWEB)

Takeuchi, A. (Toyama Univ., Toyama (Japan). College of Liberal Arts)

1991-08-25

Central Japan is the boundary region among the Northeast Japan arc (NEJ), which was parted from the Honshu arc accompanying the basin in the Japan sea area and the bending of Japan Islands, the Southwest Japan arc (SWJ) and the end of the Izu-Bonin arc (IOA), and involves the specific volcanic activity. In this report, the geohistory of the Late Cainozoic era in the central Japan was re-arranged and the neotectonics of the central Japan was tried to reorganize. Especially from a viewpoint that the central Japan is a juncture between the north end of the volcanic arc IOA (Fuji, Norikura and Hakusan volcanic belts) and the southwest end of the volcanic arc NEJ (Nasu and Chokai volcanic belts), the relation between the volcanic activity and tectonics from the Pliocene to Pleistocene has been mainly handled in the Hida region east of the Hakusan and in the northern Fossa Magna region. In addition to it, the tectonics was pointed out to be an indication of mechanical jointing state with IOA. Then the results of IOA drilling according to ODP, Leg 129 were referred as well, in order to compare with the transition of more typical volcanic activity in IOA. 61 refs., 4 figs.

Maternal seric alpha-fetoprotein: determination of the medium values in Costa Rican pregnant women

International Nuclear Information System (INIS)

Salas Chaves, P.; Villalobos Castro, A.

1999-01-01

The present study focuses on the normal mean values of AFP in 124 Costa rican pregnant women. In the women 554 determinations were done between the fifteenth and twentieth week of gestation. For this purpose, and Irma test and normal values were established. The concentration values of AFP were expressed as multiples of the median, considering a value of 2.5 Mm as the normal superior limit and of 0.25 as the inferior limit. The confidence limits of the median were 24-57 Ku/I and the 2.5MM value located between 60-143 Ku/I. (author)