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Sample records for chromosomal domain organisation

  1. Protein domain organisation: adding order

    Directory of Open Access Journals (Sweden)

    Kummerfeld Sarah K

    2009-01-01

    Full Text Available Abstract Background Domains are the building blocks of proteins. During evolution, they have been duplicated, fused and recombined, to produce proteins with novel structures and functions. Structural and genome-scale studies have shown that pairs or groups of domains observed together in a protein are almost always found in only one N to C terminal order and are the result of a single recombination event that has been propagated by duplication of the multi-domain unit. Previous studies of domain organisation have used graph theory to represent the co-occurrence of domains within proteins. We build on this approach by adding directionality to the graphs and connecting nodes based on their relative order in the protein. Most of the time, the linear order of domains is conserved. However, using the directed graph representation we have identified non-linear features of domain organization that are over-represented in genomes. Recognising these patterns and unravelling how they have arisen may allow us to understand the functional relationships between domains and understand how the protein repertoire has evolved. Results We identify groups of domains that are not linearly conserved, but instead have been shuffled during evolution so that they occur in multiple different orders. We consider 192 genomes across all three kingdoms of life and use domain and protein annotation to understand their functional significance. To identify these features and assess their statistical significance, we represent the linear order of domains in proteins as a directed graph and apply graph theoretical methods. We describe two higher-order patterns of domain organisation: clusters and bi-directionally associated domain pairs and explore their functional importance and phylogenetic conservation. Conclusion Taking into account the order of domains, we have derived a novel picture of global protein organization. We found that all genomes have a higher than expected

  2. Chromatin Domains: The Unit of Chromosome Organization.

    Science.gov (United States)

    Dixon, Jesse R; Gorkin, David U; Ren, Bing

    2016-06-01

    How eukaryotic chromosomes fold inside the nucleus is an age-old question that remains unanswered today. Early biochemical and microscopic studies revealed the existence of chromatin domains and loops as a pervasive feature of interphase chromosomes, but the biological implications of such organizational features were obscure. Genome-wide analysis of pair-wise chromatin interactions using chromatin conformation capture (3C)-based techniques has shed new light on the organization of chromosomes in interphase nuclei. Particularly, the finding of cell-type invariant, evolutionarily conserved topologically associating domains (TADs) in a broad spectrum of cell types has provided a new molecular framework for the study of animal development and human diseases. Here, we review recent progress in characterization of such chromatin domains and delineation of mechanisms of their formation in animal cells. PMID:27259200

  3. Polymer physics of chromosome large-scale 3D organisation

    Science.gov (United States)

    Chiariello, Andrea M.; Annunziatella, Carlo; Bianco, Simona; Esposito, Andrea; Nicodemi, Mario

    2016-07-01

    Chromosomes have a complex architecture in the cell nucleus, which serves vital functional purposes, yet its structure and folding mechanisms remain still incompletely understood. Here we show that genome-wide chromatin architecture data, as mapped by Hi-C methods across mammalian cell types and chromosomes, are well described by classical scaling concepts of polymer physics, from the sub-Mb to chromosomal scales. Chromatin is a complex mixture of different regions, folded in the conformational classes predicted by polymer thermodynamics. The contact matrix of the Sox9 locus, a region linked to severe human congenital diseases, is derived with high accuracy in mESCs and its molecular determinants identified by the theory; Sox9 self-assembles hierarchically in higher-order domains, involving abundant many-body contacts. Our approach is also applied to the Bmp7 locus. Finally, the model predictions on the effects of mutations on folding are tested against available data on a deletion in the Xist locus. Our results can help progressing new diagnostic tools for diseases linked to chromatin misfolding.

  4. Polymer physics of chromosome large-scale 3D organisation

    Science.gov (United States)

    Chiariello, Andrea M.; Annunziatella, Carlo; Bianco, Simona; Esposito, Andrea; Nicodemi, Mario

    2016-01-01

    Chromosomes have a complex architecture in the cell nucleus, which serves vital functional purposes, yet its structure and folding mechanisms remain still incompletely understood. Here we show that genome-wide chromatin architecture data, as mapped by Hi-C methods across mammalian cell types and chromosomes, are well described by classical scaling concepts of polymer physics, from the sub-Mb to chromosomal scales. Chromatin is a complex mixture of different regions, folded in the conformational classes predicted by polymer thermodynamics. The contact matrix of the Sox9 locus, a region linked to severe human congenital diseases, is derived with high accuracy in mESCs and its molecular determinants identified by the theory; Sox9 self-assembles hierarchically in higher-order domains, involving abundant many-body contacts. Our approach is also applied to the Bmp7 locus. Finally, the model predictions on the effects of mutations on folding are tested against available data on a deletion in the Xist locus. Our results can help progressing new diagnostic tools for diseases linked to chromatin misfolding. PMID:27405443

  5. Formation of Chromosomal Domains by Loop Extrusion.

    Science.gov (United States)

    Fudenberg, Geoffrey; Imakaev, Maxim; Lu, Carolyn; Goloborodko, Anton; Abdennur, Nezar; Mirny, Leonid A

    2016-05-31

    Topologically associating domains (TADs) are fundamental structural and functional building blocks of human interphase chromosomes, yet the mechanisms of TAD formation remain unclear. Here, we propose that loop extrusion underlies TAD formation. In this process, cis-acting loop-extruding factors, likely cohesins, form progressively larger loops but stall at TAD boundaries due to interactions with boundary proteins, including CTCF. Using polymer simulations, we show that this model produces TADs and finer-scale features of Hi-C data. Each TAD emerges from multiple loops dynamically formed through extrusion, contrary to typical illustrations of single static loops. Loop extrusion both explains diverse experimental observations-including the preferential orientation of CTCF motifs, enrichments of architectural proteins at TAD boundaries, and boundary deletion experiments-and makes specific predictions for the depletion of CTCF versus cohesin. Finally, loop extrusion has potentially far-ranging consequences for processes such as enhancer-promoter interactions, orientation-specific chromosomal looping, and compaction of mitotic chromosomes. PMID:27210764

  6. Formation of Chromosomal Domains by Loop Extrusion

    Directory of Open Access Journals (Sweden)

    Geoffrey Fudenberg

    2016-05-01

    Full Text Available Topologically associating domains (TADs are fundamental structural and functional building blocks of human interphase chromosomes, yet the mechanisms of TAD formation remain unclear. Here, we propose that loop extrusion underlies TAD formation. In this process, cis-acting loop-extruding factors, likely cohesins, form progressively larger loops but stall at TAD boundaries due to interactions with boundary proteins, including CTCF. Using polymer simulations, we show that this model produces TADs and finer-scale features of Hi-C data. Each TAD emerges from multiple loops dynamically formed through extrusion, contrary to typical illustrations of single static loops. Loop extrusion both explains diverse experimental observations—including the preferential orientation of CTCF motifs, enrichments of architectural proteins at TAD boundaries, and boundary deletion experiments—and makes specific predictions for the depletion of CTCF versus cohesin. Finally, loop extrusion has potentially far-ranging consequences for processes such as enhancer-promoter interactions, orientation-specific chromosomal looping, and compaction of mitotic chromosomes.

  7. Spatial organization of chromatin domains and compartments in single chromosomes.

    Science.gov (United States)

    Wang, Siyuan; Su, Jun-Han; Beliveau, Brian J; Bintu, Bogdan; Moffitt, Jeffrey R; Wu, Chao-ting; Zhuang, Xiaowei

    2016-08-01

    The spatial organization of chromatin critically affects genome function. Recent chromosome-conformation-capture studies have revealed topologically associating domains (TADs) as a conserved feature of chromatin organization, but how TADs are spatially organized in individual chromosomes remains unknown. Here, we developed an imaging method for mapping the spatial positions of numerous genomic regions along individual chromosomes and traced the positions of TADs in human interphase autosomes and X chromosomes. We observed that chromosome folding deviates from the ideal fractal-globule model at large length scales and that TADs are largely organized into two compartments spatially arranged in a polarized manner in individual chromosomes. Active and inactive X chromosomes adopt different folding and compartmentalization configurations. These results suggest that the spatial organization of chromatin domains can change in response to regulation. PMID:27445307

  8. Stretching the rules: monocentric chromosomes with multiple centromere domains.

    Science.gov (United States)

    Neumann, Pavel; Navrátilová, Alice; Schroeder-Reiter, Elizabeth; Koblížková, Andrea; Steinbauerová, Veronika; Chocholová, Eva; Novák, Petr; Wanner, Gerhard; Macas, Jiří

    2012-01-01

    The centromere is a functional chromosome domain that is essential for faithful chromosome segregation during cell division and that can be reliably identified by the presence of the centromere-specific histone H3 variant CenH3. In monocentric chromosomes, the centromere is characterized by a single CenH3-containing region within a morphologically distinct primary constriction. This region usually spans up to a few Mbp composed mainly of centromere-specific satellite DNA common to all chromosomes of a given species. In holocentric chromosomes, there is no primary constriction; the centromere is composed of many CenH3 loci distributed along the entire length of a chromosome. Using correlative fluorescence light microscopy and high-resolution electron microscopy, we show that pea (Pisum sativum) chromosomes exhibit remarkably long primary constrictions that contain 3-5 explicit CenH3-containing regions, a novelty in centromere organization. In addition, we estimate that the size of the chromosome segment delimited by two outermost domains varies between 69 Mbp and 107 Mbp, several factors larger than any known centromere length. These domains are almost entirely composed of repetitive DNA sequences belonging to 13 distinct families of satellite DNA and one family of centromeric retrotransposons, all of which are unevenly distributed among pea chromosomes. We present the centromeres of Pisum as novel "meta-polycentric" functional domains. Our results demonstrate that the organization and DNA composition of functional centromere domains can be far more complex than previously thought, do not require single repetitive elements, and do not require single centromere domains in order to segregate properly. Based on these findings, we propose Pisum as a useful model for investigation of centromere architecture and the still poorly understood role of repetitive DNA in centromere evolution, determination, and function. PMID:22737088

  9. Evolutionary origin and genomic organisation of runt-domain containing genes in arthropods

    Directory of Open Access Journals (Sweden)

    Smith James M

    2008-11-01

    Full Text Available Abstract Background Gene clusters, such as the Hox gene cluster, are known to have critical roles in development. In eukaryotes gene clusters arise primarily by tandem gene duplication and divergence. Genes within a cluster are often co-regulated, providing selective pressure to maintain the genome organisation, and this co-regulation can result in temporal or spatial co-linearity of gene expression. It has been previously noted that in Drosophila melanogaster, three of the four runt-domain (RD containing genes are found in a relatively tight cluster on chromosome 1, raising the possibility of a putative functional RD gene cluster in D. melanogaster. Results To investigate the possibility of such a gene cluster, orthologues of the Drosophila melanogaster RD genes were identified in several endopterygotan insects, two exopterygotan insects and two non-insect arthropods. In all insect species four RD genes were identified and orthology was assigned to the Drosophila sequences by phylogenetic analyses. Although four RD genes were found in the crustacean D. pulex, orthology could not be assigned to the insect sequences, indicating independent gene duplications from a single ancestor following the split of the hexapod lineage from the crustacean lineage. In insects, two chromosomal arrangements of these genes was observed; the first a semi-dispersed cluster, such as in Drosophila, where lozenge is separated from the core cluster of three RD genes often by megabases of DNA. The second arrangement was a tight cluster of the four RD genes, such as in Apis mellifera. This genomic organisation, particularly of the three core RD genes, raises the possibility of shared regulatory elements. In situ hybridisation of embryonic expression of the four RD genes in Drosophila melanogaster and the honeybee A. mellifera shows no evidence for either spatial or temporal co-linearity of expression during embryogenesis. Conclusion All fully sequenced insect genomes

  10. Organisation

    DEFF Research Database (Denmark)

    Lindkvist, Pia

    Du vil som læser få et indblik i, hvordan omgivelsernes ændrede krav til virksomhederne ændrer på organisations- og ledelsesteorien. Baggrunden for ”Organisation – videregående uddannelser” er, at give dig egenskaberne til at analysere og vurdere ledelsesmæssige og organisatoriske problemer...

  11. Organisation

    DEFF Research Database (Denmark)

    Sørensen, Henrik B.; Hansen, Kaj; Heide, Asbjørn;

    Bogen giver en indføring i de centrale emner indenfor organisation og ledelse. Bogen henvender sig især til akademi- og diplomuddannelserne samt uddannelser til professionsbachelorer, der alle har et anvendelsesorienteret formål. Endvidere henvender bogen sig til uddannelser og kurser, der...... beskæftiger sig med ledelse og organisation på et videregående niveau. Til bogen er der udarbejdet en lang række supplerende materialer til undervisere og studerende i form af opgaver og cases, test med tilhørende svar, vejledninger i opgaveløsning og projektarbejde. dette univers kan findes på bogens...... hjemmeside på www.organisation.academica.dk...

  12. Formation of chromosomal domains in interphase by loop extrusion

    Science.gov (United States)

    Fudenberg, Geoffrey

    While genomes are often considered as one-dimensional sequences, interphase chromosomes are organized in three dimensions with an essential role for regulating gene expression. Recent studies have shown that Topologically Associating Domains (TADs) are fundamental structural and functional building blocks of human interphase chromosomes. Despite observations that architectural proteins, including CTCF, demarcate and maintain the borders of TADs, the mechanisms underlying TAD formation remain unknown. Here we propose that loop extrusion underlies the formation TADs. In this process, cis-acting loop-extruding factors, likely cohesins, form progressively larger loops, but stall at TAD boundaries due to interactions with boundary proteins, including CTCF. This process dynamically forms loops of various sizes within but not between TADs. Using polymer simulations, we find that loop extrusion can produce TADs as determined by our analyses of the highest-resolution experimental data. Moreover, we find that loop extrusion can explain many diverse experimental observations, including: the preferential orientation of CTCF motifs and enrichments of architectural proteins at TAD boundaries; TAD boundary deletion experiments; and experiments with knockdown or depletion of CTCF, cohesin, and cohesin-loading factors. Together, the emerging picture from our work is that TADs are formed by rapidly associating, growing, and dissociating loops, presenting a clear framework for understanding interphase chromosomal organization.

  13. Transcription rate and transcript length drive formation of chromosomal interaction domain boundaries.

    Science.gov (United States)

    Le, Tung Bk; Laub, Michael T

    2016-07-15

    Chromosomes in all organisms are highly organized and divided into multiple chromosomal interaction domains, or topological domains. Regions of active, high transcription help establish and maintain domain boundaries, but precisely how this occurs remains unclear. Here, using fluorescence microscopy and chromosome conformation capture in conjunction with deep sequencing (Hi-C), we show that in Caulobacter crescentus, both transcription rate and transcript length, independent of concurrent translation, drive the formation of domain boundaries. We find that long, highly expressed genes do not form topological boundaries simply through the inhibition of supercoil diffusion. Instead, our results support a model in which long, active regions of transcription drive local decompaction of the chromosome, with these more open regions of the chromosome forming spatial gaps in vivo that diminish contacts between DNA in neighboring domains. These insights into the molecular forces responsible for domain formation in Caulobacter likely generalize to other bacteria and possibly eukaryotes. PMID:27288403

  14. Active chromatin and transcription play a key role in chromosome partitioning into topologically associating domains

    OpenAIRE

    Ulianov, Sergey V; Khrameeva, Ekaterina E.; Gavrilov, Alexey A.; Flyamer, Ilya M.; Kos, Pavel; Mikhaleva, Elena A.; Penin, Aleksey A.; Logacheva, Maria D.; Imakaev, Maxim V.; Chertovich, Alexander; Gelfand, Mikhail S; Shevelyov, Yuri Y.; Razin, Sergey V.

    2016-01-01

    Recent advances enabled by the Hi-C technique have unraveled many principles of chromosomal folding that were subsequently linked to disease and gene regulation. In particular, Hi-C revealed that chromosomes of animals are organized into topologically associating domains (TADs), evolutionary conserved compact chromatin domains that influence gene expression. Mechanisms that underlie partitioning of the genome into TADs remain poorly understood. To explore principles of TAD folding in Drosophi...

  15. Evolution of NMDA receptor cytoplasmic interaction domains: implications for organisation of synaptic signalling complexes

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    Emes Richard D

    2008-01-01

    Full Text Available Abstract Background Glutamate gated postsynaptic receptors in the central nervous system (CNS are essential for environmentally stimulated behaviours including learning and memory in both invertebrates and vertebrates. Though their genetics, biochemistry, physiology, and role in behaviour have been intensely studied in vitro and in vivo, their molecular evolution and structural aspects remain poorly understood. To understand how these receptors have evolved different physiological requirements we have investigated the molecular evolution of glutamate gated receptors and ion channels, in particular the N-methyl-D-aspartate (NMDA receptor, which is essential for higher cognitive function. Studies of rodent NMDA receptors show that the C-terminal intracellular domain forms a signalling complex with enzymes and scaffold proteins, which is important for neuronal and behavioural plasticity Results The vertebrate NMDA receptor was found to have subunits with C-terminal domains up to 500 amino acids longer than invertebrates. This extension was specific to the NR2 subunit and occurred before the duplication and subsequent divergence of NR2 in the vertebrate lineage. The shorter invertebrate C-terminus lacked vertebrate protein interaction motifs involved with forming a signaling complex although the terminal PDZ interaction domain was conserved. The vertebrate NR2 C-terminal domain was predicted to be intrinsically disordered but with a conserved secondary structure. Conclusion We highlight an evolutionary adaptation specific to vertebrate NMDA receptor NR2 subunits. Using in silico methods we find that evolution has shaped the NMDA receptor C-terminus into an unstructured but modular intracellular domain that parallels the expansion in complexity of an NMDA receptor signalling complex in the vertebrate lineage. We propose the NR2 C-terminus has evolved to be a natively unstructured yet flexible hub organising postsynaptic signalling. The evolution of

  16. Distribution of segmental duplications in the context of higher order chromatin organisation of human chromosome 7

    DEFF Research Database (Denmark)

    Ebert, Grit; Steininger, Anne; Weißmann, Robert;

    2014-01-01

    of the Williams-Beuren syndrome locus we demonstrate by cross-species comparison that these SDs have inserted at the borders of a topological domain and that they flank regions with distinct DNA conformation. CONCLUSIONS: Our study suggests a link of nuclear architecture and the propagation of SDs across...

  17. Visualization of the chromosome scaffold and intermediates of loop domain compaction in extracted mitotic cells.

    Science.gov (United States)

    Sheval, Eugene V; Polyakov, Vladimir Y

    2006-12-01

    A novel extraction protocol for cells cultured on coverslips is described. Observations of the extraction process in a perfusion chamber reveal that cells of all mitotic stages are not detached from coverslips during extraction, and all stages can be recognized using phase contrast images. We studied the extracted cell morphology and distribution of a major scaffold component - topoisomerase IIalpha, in extracted metaphase and anaphase cells. An extraction using 2M NaCl leads to destruction of chromosomes at the light microscope level. Immunogold studies demonstrate that the only residual structure observed is an axial chromosome scaffold that contains topoisomerase IIalpha. In contrast, mitotic chromosomes are swelled only partially after an extraction using dextran sulphate and heparin, and it appears that this treatment does not lead to total destruction of loop domains. In this case, the chromosome scaffold and numerous structures resembling small rosettes are revealed inside extracted cells. The rosettes observed condense after addition of Mg2+-ions and do not contain topoisomerase IIalpha suggesting that these structures correspond to intermediates of loop domain compaction. We propose a model of chromosome structure in which the loop domains are condensed into highly regular structures with rosette organization. PMID:17029868

  18. Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions.

    Science.gov (United States)

    Guelen, Lars; Pagie, Ludo; Brasset, Emilie; Meuleman, Wouter; Faza, Marius B; Talhout, Wendy; Eussen, Bert H; de Klein, Annelies; Wessels, Lodewyk; de Laat, Wouter; van Steensel, Bas

    2008-06-12

    The architecture of human chromosomes in interphase nuclei is still largely unknown. Microscopy studies have indicated that specific regions of chromosomes are located in close proximity to the nuclear lamina (NL). This has led to the idea that certain genomic elements may be attached to the NL, which may contribute to the spatial organization of chromosomes inside the nucleus. However, sequences in the human genome that interact with the NL in vivo have not been identified. Here we construct a high-resolution map of the interaction sites of the entire genome with NL components in human fibroblasts. This map shows that genome-lamina interactions occur through more than 1,300 sharply defined large domains 0.1-10 megabases in size. These lamina-associated domains (LADs) are typified by low gene-expression levels, indicating that LADs represent a repressive chromatin environment. The borders of LADs are demarcated by the insulator protein CTCF, by promoters that are oriented away from LADs, or by CpG islands, suggesting possible mechanisms of LAD confinement. Taken together, these results demonstrate that the human genome is divided into large, discrete domains that are units of chromosome organization within the nucleus. PMID:18463634

  19. Spatial relationship between chromosomal domains in diploid and autotetraploid Arabidopsis thaliana nuclei.

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    Sas-Nowosielska, H; Bernas, T

    2016-04-25

    Polyploids constitute more than 80% of angiosperm plant species. Their DNA content is often further increased by endoreplication, which occurs as a part of cell differentiation. Here, we explore the relationship between 3D chromatin architecture, number of genome copies and their origin in the model plant, Arabidopsis thaliana. Spatial proximity between pericentromeric, interstitial and subtelomeric domains of chromosomes 1 and 4 was quantified over a range of distances. The results indicate that average nuclear volume as well as chromatin density increase with the genome copy number. Similar dependence is observed when association of homologous chromosomes (in 2C/ endopolyploid nuclei) and sister chromatid separation (in endopolyploid nuclei) is studied. Moreover, clusters of chromosomal domains are detectable at the spatial scale above microscopy resolution. Subtelomeric, interstitial and pericentromeric chromosomal domains are affected to different extent by these processes, which are modulated by endopolyploidy. This factor influences fusion of heterochromatin as well. Nonetheless, local chromatin architecture of Arabidopsis thaliana depends mainly on endopolyploidy level, and to lesser extend on polyploidy. PMID:27310308

  20. Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation.

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    Georgina Gómez-Saldivar

    2016-06-01

    Full Text Available Nucleoporins are the constituents of nuclear pore complexes (NPCs and are essential regulators of nucleocytoplasmic transport, gene expression and genome stability. The nucleoporin MEL-28/ELYS plays a critical role in post-mitotic NPC reassembly through recruitment of the NUP107-160 subcomplex, and is required for correct segregation of mitotic chromosomes. Here we present a systematic functional and structural analysis of MEL-28 in C. elegans early development and human ELYS in cultured cells. We have identified functional domains responsible for nuclear envelope and kinetochore localization, chromatin binding, mitotic spindle matrix association and chromosome segregation. Surprisingly, we found that perturbations to MEL-28's conserved AT-hook domain do not affect MEL-28 localization although they disrupt MEL-28 function and delay cell cycle progression in a DNA damage checkpoint-dependent manner. Our analyses also uncover a novel meiotic role of MEL-28. Together, these results show that MEL-28 has conserved structural domains that are essential for its fundamental roles in NPC assembly and chromosome segregation.

  1. Nucleonica: a platform for organisational knowledge management in the nuclear domain

    Energy Technology Data Exchange (ETDEWEB)

    Magill, J. [Institute for Transuranium Elements, European Commission Joint Research Centre, Postfach 2340, 76125 Karlsruhe (Germany); Magill, N.F. [Department of Management, Gilbert Scott Building, Glasgow G12 8QQ, Scotland (United Kingdom)

    2010-07-01

    The nuclear science web portal Nucleonica is considered from a knowledge management perspective. In particular, Nucleonica's 'knowledge objects' are considered within the context of Nonaka's 'knowledge spiral' model for organisational knowledge creation, transfer and dissemination. (authors)

  2. Chromosome

    Science.gov (United States)

    Chromosomes are structures found in the center (nucleus) of cells that carry long pieces of DNA. DNA ... is the building block of the human body. Chromosomes also contain proteins that help DNA exist in ...

  3. Organising Sustainable Transition: Understanding the Product, Project and Service Domain of the Built Environment

    DEFF Research Database (Denmark)

    Thuesen, Christian; Koch-Ørvad, Nina; Maslesa, Esmir

    2016-01-01

    Sustainable transition of the built environment con struction industry is challenging the existing construction practices and business models. This article presents a framework for understanding and facilitating sustainable transition in the built environment. The framework was developed through...... of three generic domains - the Project, Product and Service domain - with widely different markets, companies, business models and regulation. Besides identifying the characteristics of the different domains, the findings show that these domains are interdependent, but largely live their own lives...... and built environment....

  4. Replication domains are self-interacting structural chromatin units of human chromosomes

    Science.gov (United States)

    Arneodo, Alain

    2011-03-01

    In higher eukaryotes, the absence of specific sequence motifs marking the origins of replication has been a serious hindrance to the understanding of the mechanisms that regulate the initiation and the maintenance of the replication program in different cell types. In silico analysis of nucleotide compositional skew has predicted the existence, in the germline, of replication N-domains bordered by putative replication origins and where the skew decreases rather linearly as the signature of a progressive inversion of the average fork polarity. Here, from the demonstration that the average fork polarity can be directly extracted from the derivative of replication timing profiles, we develop a wavelet-based pattern recognition methodology to delineate replication U-domains where the replication timing profile is shaped as a U and its derivative as a N. Replication U-domains are robustly found in seven cell lines as covering a significant portion (40-50%) of the human genome where the replication timing data actually displays some plasticity between cell lines. The early replication initiation zones at U-domains borders are found to be hypersensitive to DNase I cleavage, to be associated with transcriptional activity and to present a significant enrichment in insular-binding proteins CTCF, the hallmark of an open chromatin structure. A comparative analysis of genome-wide chromatin interaction (HiC) data shows that replication-U domains correspond to self-interacting structural high order chromatin units of megabase characteristic size. Taken together, these findings provide evidence that the epigenetic compartmentalization of the human genome into autonomous replication U-domains comes along with an extensive remodelling of the threedimensional chromosome architecture during development or in specific diseases. The observed cell specific conservation of the replication timing between the human and mouse genomes strongly suggests that this chromosome organization into

  5. Active chromatin and transcription play a key role in chromosome partitioning into topologically associating domains.

    Science.gov (United States)

    Ulianov, Sergey V; Khrameeva, Ekaterina E; Gavrilov, Alexey A; Flyamer, Ilya M; Kos, Pavel; Mikhaleva, Elena A; Penin, Aleksey A; Logacheva, Maria D; Imakaev, Maxim V; Chertovich, Alexander; Gelfand, Mikhail S; Shevelyov, Yuri Y; Razin, Sergey V

    2016-01-01

    Recent advances enabled by the Hi-C technique have unraveled many principles of chromosomal folding that were subsequently linked to disease and gene regulation. In particular, Hi-C revealed that chromosomes of animals are organized into topologically associating domains (TADs), evolutionary conserved compact chromatin domains that influence gene expression. Mechanisms that underlie partitioning of the genome into TADs remain poorly understood. To explore principles of TAD folding in Drosophila melanogaster, we performed Hi-C and poly(A)(+) RNA-seq in four cell lines of various origins (S2, Kc167, DmBG3-c2, and OSC). Contrary to previous studies, we find that regions between TADs (i.e., the inter-TADs and TAD boundaries) in Drosophila are only weakly enriched with the insulator protein dCTCF, while another insulator protein Su(Hw) is preferentially present within TADs. However, Drosophila inter-TADs harbor active chromatin and constitutively transcribed (housekeeping) genes. Accordingly, we find that binding of insulator proteins dCTCF and Su(Hw) predicts TAD boundaries much worse than active chromatin marks do. Interestingly, inter-TADs correspond to decompacted inter-bands of polytene chromosomes, whereas TADs mostly correspond to densely packed bands. Collectively, our results suggest that TADs are condensed chromatin domains depleted in active chromatin marks, separated by regions of active chromatin. We propose the mechanism of TAD self-assembly based on the ability of nucleosomes from inactive chromatin to aggregate, and lack of this ability in acetylated nucleosomal arrays. Finally, we test this hypothesis by polymer simulations and find that TAD partitioning may be explained by different modes of inter-nucleosomal interactions for active and inactive chromatin. PMID:26518482

  6. Comparative Anatomy of Chromosomal Domains with Imprinted and Non-Imprinted Allele-Specific DNA Methylation

    OpenAIRE

    Paliwal, Anupam; Temkin, Alexis M.; Kerkel, Kristi; Yale, Alexander; Yotova, Iveta; Drost, Natalia; Lax, Simon; Nhan-Chang, Chia-Ling; Powell, Charles; Borczuk, Alain; Aviv, Abraham; Wapner, Ronald; Chen, Xiaowei; Nagy, Peter L.; Schork, Nicholas,

    2013-01-01

    Allele-specific DNA methylation (ASM) is well studied in imprinted domains, but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci, where the ASM is dictated not by parent-of-origin but instead by the local haplotype. We identified loci with strong ASM in human tissues from methylation-sensitive SNP array data. Two index regions (bisulfite PCR amplicons), one between the C3orf27 and RPN1 genes in chromosome band 3q21 and the other near the VTRNA2-1 vault R...

  7. Comparative anatomy of chromosomal domains with imprinted and non-imprinted allele-specific DNA methylation.

    OpenAIRE

    Anupam Paliwal; Temkin, Alexis M.; Kristi Kerkel; Alexander Yale; Iveta Yotova; Natalia Drost; Simon Lax; Chia-Ling Nhan-Chang; Charles Powell; Alain Borczuk; Abraham Aviv; Ronald Wapner; Xiaowei Chen; Nagy, Peter L.; Nicholas Schork

    2013-01-01

    Allele-specific DNA methylation (ASM) is well studied in imprinted domains, but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci, where the ASM is dictated not by parent-of-origin but instead by the local haplotype. We identified loci with strong ASM in human tissues from methylation-sensitive SNP array data. Two index regions (bisulfite PCR amplicons), one between the C3orf27 and RPN1 genes in chromosome band 3q21 and the other near the VTRNA2-1 vault R...

  8. Comparative anatomy of chromosomal domains with imprinted and non-imprinted allele-specific DNA methylation.

    Directory of Open Access Journals (Sweden)

    Anupam Paliwal

    2013-08-01

    Full Text Available Allele-specific DNA methylation (ASM is well studied in imprinted domains, but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci, where the ASM is dictated not by parent-of-origin but instead by the local haplotype. We identified loci with strong ASM in human tissues from methylation-sensitive SNP array data. Two index regions (bisulfite PCR amplicons, one between the C3orf27 and RPN1 genes in chromosome band 3q21 and the other near the VTRNA2-1 vault RNA in band 5q31, proved to be new examples of imprinted DMRs (maternal alleles methylated while a third, between STEAP3 and C2orf76 in chromosome band 2q14, showed non-imprinted haplotype-dependent ASM. Using long-read bisulfite sequencing (bis-seq in 8 human tissues we found that in all 3 domains the ASM is restricted to single differentially methylated regions (DMRs, each less than 2kb. The ASM in the C3orf27-RPN1 intergenic region was placenta-specific and associated with allele-specific expression of a long non-coding RNA. Strikingly, the discrete DMRs in all 3 regions overlap with binding sites for the insulator protein CTCF, which we found selectively bound to the unmethylated allele of the STEAP3-C2orf76 DMR. Methylation mapping in two additional genes with non-imprinted haplotype-dependent ASM, ELK3 and CYP2A7, showed that the CYP2A7 DMR also overlaps a CTCF site. Thus, two features of imprinted domains, highly localized DMRs and allele-specific insulator occupancy by CTCF, can also be found in chromosomal domains with non-imprinted ASM. Arguing for biological importance, our analysis of published whole genome bis-seq data from hES cells revealed multiple genome-wide association study (GWAS peaks near CTCF binding sites with ASM.

  9. Comparative anatomy of chromosomal domains with imprinted and non-imprinted allele-specific DNA methylation.

    Science.gov (United States)

    Paliwal, Anupam; Temkin, Alexis M; Kerkel, Kristi; Yale, Alexander; Yotova, Iveta; Drost, Natalia; Lax, Simon; Nhan-Chang, Chia-Ling; Powell, Charles; Borczuk, Alain; Aviv, Abraham; Wapner, Ronald; Chen, Xiaowei; Nagy, Peter L; Schork, Nicholas; Do, Catherine; Torkamani, Ali; Tycko, Benjamin

    2013-08-01

    Allele-specific DNA methylation (ASM) is well studied in imprinted domains, but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci, where the ASM is dictated not by parent-of-origin but instead by the local haplotype. We identified loci with strong ASM in human tissues from methylation-sensitive SNP array data. Two index regions (bisulfite PCR amplicons), one between the C3orf27 and RPN1 genes in chromosome band 3q21 and the other near the VTRNA2-1 vault RNA in band 5q31, proved to be new examples of imprinted DMRs (maternal alleles methylated) while a third, between STEAP3 and C2orf76 in chromosome band 2q14, showed non-imprinted haplotype-dependent ASM. Using long-read bisulfite sequencing (bis-seq) in 8 human tissues we found that in all 3 domains the ASM is restricted to single differentially methylated regions (DMRs), each less than 2kb. The ASM in the C3orf27-RPN1 intergenic region was placenta-specific and associated with allele-specific expression of a long non-coding RNA. Strikingly, the discrete DMRs in all 3 regions overlap with binding sites for the insulator protein CTCF, which we found selectively bound to the unmethylated allele of the STEAP3-C2orf76 DMR. Methylation mapping in two additional genes with non-imprinted haplotype-dependent ASM, ELK3 and CYP2A7, showed that the CYP2A7 DMR also overlaps a CTCF site. Thus, two features of imprinted domains, highly localized DMRs and allele-specific insulator occupancy by CTCF, can also be found in chromosomal domains with non-imprinted ASM. Arguing for biological importance, our analysis of published whole genome bis-seq data from hES cells revealed multiple genome-wide association study (GWAS) peaks near CTCF binding sites with ASM. PMID:24009515

  10. Organisation Matters

    DEFF Research Database (Denmark)

    Unphon, Hataichanok; Dittrich, Yvonne

    2008-01-01

    Our work aims at understanding the design rationale for product line architecture by focusing on the design of common data access modules for complex simulation software products. This paper presents empirical evidence of organisational and business domain aspects that influence the development o...... of product line architecture. We suggest that the assessment of use-situation and his tory of organisational structure should be considered when creating product line architectures, especially for products that are tailored and used interactively.......Our work aims at understanding the design rationale for product line architecture by focusing on the design of common data access modules for complex simulation software products. This paper presents empirical evidence of organisational and business domain aspects that influence the development...

  11. SAP-like domain in nucleolar spindle associated protein mediates mitotic chromosome loading as well as interphase chromatin interaction

    Energy Technology Data Exchange (ETDEWEB)

    Verbakel, Werner, E-mail: werner.verbakel@chem.kuleuven.be [Laboratory of Biomolecular Dynamics, Katholieke Universiteit Leuven, Celestijnenlaan 200G, Bus 2403, 3001 Heverlee (Belgium); Carmeliet, Geert, E-mail: geert.carmeliet@med.kuleuven.be [Laboratory of Experimental Medicine and Endocrinology, Katholieke Universiteit Leuven, Herestraat 49, Bus 902, 3000 Leuven (Belgium); Engelborghs, Yves, E-mail: yves.engelborghs@fys.kuleuven.be [Laboratory of Biomolecular Dynamics, Katholieke Universiteit Leuven, Celestijnenlaan 200G, Bus 2403, 3001 Heverlee (Belgium)

    2011-08-12

    Highlights: {yields} The SAP-like domain in NuSAP is a functional DNA-binding domain with preference for dsDNA. {yields} This SAP-like domain is essential for chromosome loading during early mitosis. {yields} NuSAP is highly dynamic on mitotic chromatin, as evident from photobleaching experiments. {yields} The SAP-like domain also mediates NuSAP-chromatin interaction in interphase nucleoplasm. -- Abstract: Nucleolar spindle associated protein (NuSAP) is a microtubule-stabilizing protein that localizes to chromosome arms and chromosome-proximal microtubules during mitosis and to the nucleus, with enrichment in the nucleoli, during interphase. The critical function of NuSAP is underscored by the finding that its depletion in HeLa cells results in various mitotic defects. Moreover, NuSAP is found overexpressed in multiple cancers and its expression levels often correlate with the aggressiveness of cancer. Due to its localization on chromosome arms and combination of microtubule-stabilizing and DNA-binding properties, NuSAP takes a special place within the extensive group of spindle assembly factors. In this study, we identify a SAP-like domain that shows DNA binding in vitro with a preference for dsDNA. Deletion of the SAP-like domain abolishes chromosome arm binding of NuSAP during mitosis, but is not sufficient to abrogate its chromosome-proximal localization after anaphase onset. Fluorescence recovery after photobleaching experiments revealed the highly dynamic nature of this NuSAP-chromatin interaction during mitosis. In interphase cells, NuSAP also interacts with chromatin through its SAP-like domain, as evident from its enrichment on dense chromatin regions and intranuclear mobility, measured by fluorescence correlation spectroscopy. The obtained results are in agreement with a model where NuSAP dynamically stabilizes newly formed microtubules on mitotic chromosomes to enhance chromosome positioning without immobilizing these microtubules. Interphase Nu

  12. High-Throughput Live-Cell Microscopy Analysis of Association Between Chromosome Domains and the Nucleolus in S. cerevisiae.

    Science.gov (United States)

    Wang, Renjie; Normand, Christophe; Gadal, Olivier

    2016-01-01

    Spatial organization of the genome has important impacts on all aspects of chromosome biology, including transcription, replication, and DNA repair. Frequent interactions of some chromosome domains with specific nuclear compartments, such as the nucleolus, are now well documented using genome-scale methods. However, direct measurement of distance and interaction frequency between loci requires microscopic observation of specific genomic domains and the nucleolus, followed by image analysis to allow quantification. The fluorescent repressor operator system (FROS) is an invaluable method to fluorescently tag DNA sequences and investigate chromosome position and dynamics in living cells. This chapter describes a combination of methods to define motion and region of confinement of a locus relative to the nucleolus in cell's nucleus, from fluorescence acquisition to automated image analysis using two dedicated pipelines. PMID:27576709

  13. High-Throughput Live-Cell Microscopy Analysis of Association Between Chromosome Domains and the Nucleolus in S. cerevisiae.

    Science.gov (United States)

    Wang, Renjie; Normand, Christophe; Gadal, Olivier

    2016-01-01

    Spatial organization of the genome has important impacts on all aspects of chromosome biology, including transcription, replication, and DNA repair. Frequent interactions of some chromosome domains with specific nuclear compartments, such as the nucleolus, are now well documented using genome-scale methods. However, direct measurement of distance and interaction frequency between loci requires microscopic observation of specific genomic domains and the nucleolus, followed by image analysis to allow quantification. The fluorescent repressor operator system (FROS) is an invaluable method to fluorescently tag DNA sequences and investigate chromosome position and dynamics in living cells. This chapter describes a combination of methods to define motion and region of confinement of a locus relative to the nucleolus in cell's nucleus, from fluorescence acquisition to automated image analysis using two dedicated pipelines.

  14. Imprinted chromosomal domains revealed by allele-specific replication timing of the GABRB3 and GABRA5 genes

    Energy Technology Data Exchange (ETDEWEB)

    LaSalle, J.; Flint, A.; Lalande, M. [Harvard Medical School, Boston, MA (United States)] [and others

    1994-09-01

    The GABRB3 and GABRA5 genes are organized as a cluster in chromosome 15q11-q13. The genes are separated by around 100 kb and arranged in opposite transcriptional orientations. The GABA{sub A} receptor cluster lies near the Angelman and Prader-Willi loci and displays asynchronous DNA replication, suggesting that this region is subject to parental imprinting. In order to further study the association between DNA replication and imprinting, allele-specific replication was assayed by fluorescence in situ hybridization with {lambda}-phage probes from the GABRB3/A5 region and a D15Z1 satellite probe to identify the parental origin of each chromosome. The replication kinetics of each allele was determined by using a flow sorter to fractionate mitogen-stimulated lymphocytes on the basis of cell cycle progression prior to FISH analysis. These kinetic studies reveal a 50-150 kb chromosomal domain extending from the middle of the GABRB3/A5 intergenic region into the GABRA5 5{prime}-UTR which displays maternal replication in early S with paternal replication delayed until the end of S. In contrast, genomic regions on either side of this maternal early replication domain exhibit the opposite pattern with paternal before maternal replication and both alleles replicating in the latter half of S. These results indicate that the GABRB3/A5 region is divided into domains in which replication timing is determined by parental origin. In addition to a loss of asynchronous replication, organization into replication timing domains is also lost in lymphocytes from maternal and paternal uniparental disomy 15 patients suggesting that a chromosome contribution from both parents is required for the establishment of the imprinted replication domains.

  15. The HhH2/NDD domain of the Drosophila Nod chromokinesin-like protein is required for binding to chromosomes in the oocyte nucleus.

    Science.gov (United States)

    Cui, Wei; Hawley, R Scott

    2005-12-01

    Nod is a chromokinesin-like protein that plays a critical role in segregating achiasmate chromosomes during female meiosis. The C-terminal half of the Nod protein contains two putative DNA-binding domains. The first of these domains, known as the HMGN domain, consists of three tandemly repeated high-mobility group N motifs. This domain was previously shown to be both necessary and sufficient for binding of the C-terminal half of Nod to mitotic chromosomes in embryos. The second putative DNA-binding domain, denoted HhH(2)/NDD, is a helix-hairpin-helix(2)/Nod-like DNA-binding domain. Although the HhH(2)/NDD domain is not required or sufficient for chromosome binding in embryos, several well-characterized nod mutations have been mapped in this domain. To characterize the role of the HhH(2)/NDD domain in mediating Nod function, we created a series of UAS-driven transgene constructs capable of expressing either a wild-type Nod-GFP fusion protein or proteins in which the HhH(2)/NDD domain had been altered by site-directed mutagenesis. Although wild-type Nod-GFP localizes to the oocyte chromosomes and rescues the segregation defect in nod mutant oocytes, two of three proteins carrying mutants in the HhH(2)/NDD domain fail to either rescue the nod mutant phenotype or bind to oocyte chromosomes. However, these mutant proteins do bind to the polytene chromosomes in nurse-cell nuclei and enter the oocyte nucleus. Thus, even though the HhH(2)/NDD domain is not essential for chromosome binding in other cell types, it is required for chromosome binding in the oocyte. These HhH(2)/NDD mutants also block the localization of Nod to the posterior pole of stage 9-10A oocytes, a process that is thought to facilitate the interaction of Nod with the plus ends of microtubules (Cui et al. 2005). This observation suggests that the Nod HhH2/NDD domain may play other roles in addition to binding Nod to meiotic chromosomes.

  16. Domain Specific Attentional Impairments in Children with Chromosome 22Q11.2 Deletion Syndrome

    Science.gov (United States)

    Bish, Joel P.; Chiodo, Renee; Mattei, Victoria; Simon, Tony J.

    2007-01-01

    One of the defining cognitive characteristics of the chromosome 22q deletion syndrome (DS22q11.2) is visuospatial processing impairments. The purpose of this study was to investigate and extend the specific attentional profile of children with this disorder using both an object-based attention task and an inhibition of return task. A group of…

  17. NMR spectroscopic and bioinformatic analyses of the LTBP1 C-terminus reveal a highly dynamic domain organisation.

    Directory of Open Access Journals (Sweden)

    Ian B Robertson

    Full Text Available Proteins from the LTBP/fibrillin family perform key structural and functional roles in connective tissues. LTBP1 forms the large latent complex with TGFβ and its propeptide LAP, and sequesters the latent growth factor to the extracellular matrix. Bioinformatics studies suggest the main structural features of the LTBP1 C-terminus are conserved through evolution. NMR studies were carried out on three overlapping C-terminal fragments of LTBP1, comprising four domains with characterised homologues, cbEGF14, TB3, EGF3 and cbEGF15, and three regions with no homology to known structures. The NMR data reveal that the four domains adopt canonical folds, but largely lack the interdomain interactions observed with homologous fibrillin domains; the exception is the EGF3-cbEGF15 domain pair which has a well-defined interdomain interface. (15N relaxation studies further demonstrate that the three interdomain regions act as flexible linkers, allowing a wide range of motion between the well-structured domains. This work is consistent with the LTBP1 C-terminus adopting a flexible "knotted rope" structure, which may facilitate cell matrix interactions, and the accessibility to proteases or other factors that could contribute to TGFβ activation.

  18. Domain specific attentional impairments in children with chromosome 22q11.2 deletion syndrome

    OpenAIRE

    Bish, Joel P.; Chiodo, Renee; Mattei, Victoria; Simon, Tony J.

    2007-01-01

    One of the defining cognitive characteristics of the chromosome 22q deletion syndrome (DS22q11.2) is visuospatial processing impairments. The purpose of this study was to investigate and extend the specific attentional profile of children with this disorder using both an object-based attention task and an inhibition of return task. A group of children with the disorder was compared in these tasks with a group of age-matched typically developing children. The children with DS22q11.2 demonstrat...

  19. Aberrant Behaviours of Reaction Diffusion Self-organisation Models on Growing Domains in the Presence of Gene Expression Time Delays

    KAUST Repository

    Seirin Lee, S.

    2010-03-23

    Turing\\'s pattern formation mechanism exhibits sensitivity to the details of the initial conditions suggesting that, in isolation, it cannot robustly generate pattern within noisy biological environments. Nonetheless, secondary aspects of developmental self-organisation, such as a growing domain, have been shown to ameliorate this aberrant model behaviour. Furthermore, while in-situ hybridisation reveals the presence of gene expression in developmental processes, the influence of such dynamics on Turing\\'s model has received limited attention. Here, we novelly focus on the Gierer-Meinhardt reaction diffusion system considering delays due the time taken for gene expression, while incorporating a number of different domain growth profiles to further explore the influence and interplay of domain growth and gene expression on Turing\\'s mechanism. We find extensive pathological model behaviour, exhibiting one or more of the following: temporal oscillations with no spatial structure, a failure of the Turing instability and an extreme sensitivity to the initial conditions, the growth profile and the duration of gene expression. This deviant behaviour is even more severe than observed in previous studies of Schnakenberg kinetics on exponentially growing domains in the presence of gene expression (Gaffney and Monk in Bull. Math. Biol. 68:99-130, 2006). Our results emphasise that gene expression dynamics induce unrealistic behaviour in Turing\\'s model for multiple choices of kinetics and thus such aberrant modelling predictions are likely to be generic. They also highlight that domain growth can no longer ameliorate the excessive sensitivity of Turing\\'s mechanism in the presence of gene expression time delays. The above, extensive, pathologies suggest that, in the presence of gene expression, Turing\\'s mechanism would generally require a novel and extensive secondary mechanism to control reaction diffusion patterning. © 2010 Society for Mathematical Biology.

  20. Four chromo-domain proteins of Schizosaccharomyces pombe differentially repress transcription at various chromosomal locations.

    OpenAIRE

    Thon, G.; Verhein-Hansen, J.

    2000-01-01

    Transcription is repressed in regions of the fission yeast genome close to centromeres, telomeres, or the silent mating-type cassettes mat2-P and mat3-M. The repression involves the chromo-domain proteins Swi6 and Clr4. We report that two other chromo-domain proteins, Chp1 and Chp2, are also important for these position effects. Chp1 showed a specificity for centromeric regions. Its essentiality for the transcriptional repression of centromeric markers correlates with its importance for chrom...

  1. Organisational Learning

    Institute of Scientific and Technical Information of China (English)

    谢苑苑

    2014-01-01

    In the process of global economy, there is a growing demand for organisational learning. This article critically reviews four models of organisational learning and applies them to the work experience of the author.

  2. Organisational Structure

    Science.gov (United States)

    National Centre for Vocational Education Research (NCVER), 2006

    2006-01-01

    An understanding of organisational structure can provide guidance for organisations that want to change and innovate. Many writers agree that this understanding allows organisations to shape how their work is done to ultimately achieve their business goals--and that too often structure is given little consideration in business strategy and…

  3. Analysis of the chromatin domain organisation around the plastocyanin gene reveals an MAR-specific sequence element in Arabidopsis thaliana.

    Science.gov (United States)

    van Drunen, C M; Oosterling, R W; Keultjes, G M; Weisbeek, P J; van Driel, R; Smeekens, S C

    1997-10-01

    The Arabidopsis thaliana genome is currently being sequenced, eventually leading towards the unravelling of all potential genes. We wanted to gain more insight into the way this genome might be organized at the ultrastructural level. To this extent we identified matrix attachment regions demarking potential chromatin domains, in a 16 kb region around the plastocyanin gene. The region was cloned and sequenced revealing six genes in addition to the plastocyanin gene. Using an heterologous in vitro nuclear matrix binding assay, to search for evolutionary conserved matrix attachment regions (MARs), we identified three such MARs. These three MARs divide the region into two small chromatin domains of 5 kb, each containing two genes. Comparison of the sequence of the three MARs revealed a degenerated 21 bp sequence that is shared between these MARs and that is not found elsewhere in the region. A similar sequence element is also present in four other MARs of Arabidopsis.Therefore, this sequence may constitute a landmark for the position of MARs in the genome of this plant. In a genomic sequence database of Arabidopsis the 21 bp element is found approximately once every 10 kb. The compactness of the Arabidopsis genome could account for the high incidence of MARs and MRSs we observed.

  4. Parenté, alliance, organisation sociale et cohabitation religieuse pacifique dans le domaine Est indonésien

    Directory of Open Access Journals (Sweden)

    Nao-Cosme Rémon

    2012-12-01

    Full Text Available Dans cet ouvrage basé sur sa thèse de doctorat, Philipus Tule propose une étude monographique de l’ethnographie d’une société Est indonésienne religieusement mixte, les Kéo de la côte sud du centre de Florès (district Ngada. En prenant appui sur une description détaillée du système culturel local du domaine d’Udi Worowatu (qui correspond à la division administrative indonésienne actuelle desa du sous-district (kecamatan de Ma’uponggo, Tule montre comment se manifeste la coexistence interre...

  5. Organisational Art

    DEFF Research Database (Denmark)

    Ferro-Thomsen, Martin

    University of Copenhagen / Learning Lab Denmark. 2005 Kort beskrivelse: Organisational Art is a tentative title for an art form that works together with organisations to produce art. This is most often done together with non-artist members of the organisation and on-site in their social context. OA...... is characterised as socially engaged, conceptual, discursive, site-specific and contextual. Abstract: This investigation is about Organisational Art (OA), which is a tentative title for an art form that works together with organisations (companies, institutions, communities, governments and NGOs) to produce art....... This is most often done together with non-artist members of the organisation and on-site in their social context. OA is characterised as socially engaged, conceptual, discursive, site-specific and contextual. It is argued that OA seeks to advance both art and the organisation of human work/life by crossing...

  6. Zinc Finger Domain of the PRDM9 Gene on Chromosome 1 Exhibits High Diversity in Ruminants but Its Paralog PRDM7 Contains Multiple Disruptive Mutations

    Science.gov (United States)

    Ahlawat, Sonika; Sharma, Priyanka; Sharma, Rekha; Arora, Reena; De, Sachinandan

    2016-01-01

    PRDM9 is the sole hybrid sterility gene identified so far in vertebrates. PRDM9 gene encodes a protein with an immensely variable zinc-finger (ZF) domain that determines the site of meiotic recombination hotspots genome-wide. In this study, the terminal ZF domain of PRDM9 on bovine chromosome 1 and its paralog on chromosome 22 were characterized in 225 samples from five ruminant species (cattle, yak, mithun, sheep and goat). We found extraordinary variation in the number of PRDM9 zinc fingers (6 to 12). We sequenced PRDM9 ZF encoding region from 15 individuals (carrying the same ZF number in both copies) and found 43 different ZF domain sequences. Ruminant zinc fingers of PRDM9 were found to be diversifying under positive selection and concerted evolution, specifically at positions involved in defining their DNA-binding specificity, consistent with the reports from other vertebrates such as mice, humans, equids and chimpanzees. ZF-encoding regions of the PRDM7, a paralog of PRDM9 on bovine chromosome 22 and on unknown chromosomes in other studied species were found to contain 84 base repeat units as in PRDM9, but there were multiple disruptive mutations after the first repeat unit. The diversity of the ZFs suggests that PRDM9 may activate recombination hotspots that are largely unique to each ruminant species. PMID:27203728

  7. Zinc Finger Domain of the PRDM9 Gene on Chromosome 1 Exhibits High Diversity in Ruminants but Its Paralog PRDM7 Contains Multiple Disruptive Mutations.

    Science.gov (United States)

    Ahlawat, Sonika; Sharma, Priyanka; Sharma, Rekha; Arora, Reena; De, Sachinandan

    2016-01-01

    PRDM9 is the sole hybrid sterility gene identified so far in vertebrates. PRDM9 gene encodes a protein with an immensely variable zinc-finger (ZF) domain that determines the site of meiotic recombination hotspots genome-wide. In this study, the terminal ZF domain of PRDM9 on bovine chromosome 1 and its paralog on chromosome 22 were characterized in 225 samples from five ruminant species (cattle, yak, mithun, sheep and goat). We found extraordinary variation in the number of PRDM9 zinc fingers (6 to 12). We sequenced PRDM9 ZF encoding region from 15 individuals (carrying the same ZF number in both copies) and found 43 different ZF domain sequences. Ruminant zinc fingers of PRDM9 were found to be diversifying under positive selection and concerted evolution, specifically at positions involved in defining their DNA-binding specificity, consistent with the reports from other vertebrates such as mice, humans, equids and chimpanzees. ZF-encoding regions of the PRDM7, a paralog of PRDM9 on bovine chromosome 22 and on unknown chromosomes in other studied species were found to contain 84 base repeat units as in PRDM9, but there were multiple disruptive mutations after the first repeat unit. The diversity of the ZFs suggests that PRDM9 may activate recombination hotspots that are largely unique to each ruminant species. PMID:27203728

  8. Zinc Finger Domain of the PRDM9 Gene on Chromosome 1 Exhibits High Diversity in Ruminants but Its Paralog PRDM7 Contains Multiple Disruptive Mutations.

    Directory of Open Access Journals (Sweden)

    Sonika Ahlawat

    Full Text Available PRDM9 is the sole hybrid sterility gene identified so far in vertebrates. PRDM9 gene encodes a protein with an immensely variable zinc-finger (ZF domain that determines the site of meiotic recombination hotspots genome-wide. In this study, the terminal ZF domain of PRDM9 on bovine chromosome 1 and its paralog on chromosome 22 were characterized in 225 samples from five ruminant species (cattle, yak, mithun, sheep and goat. We found extraordinary variation in the number of PRDM9 zinc fingers (6 to 12. We sequenced PRDM9 ZF encoding region from 15 individuals (carrying the same ZF number in both copies and found 43 different ZF domain sequences. Ruminant zinc fingers of PRDM9 were found to be diversifying under positive selection and concerted evolution, specifically at positions involved in defining their DNA-binding specificity, consistent with the reports from other vertebrates such as mice, humans, equids and chimpanzees. ZF-encoding regions of the PRDM7, a paralog of PRDM9 on bovine chromosome 22 and on unknown chromosomes in other studied species were found to contain 84 base repeat units as in PRDM9, but there were multiple disruptive mutations after the first repeat unit. The diversity of the ZFs suggests that PRDM9 may activate recombination hotspots that are largely unique to each ruminant species.

  9. The C terminus of fragile X mental retardation protein interacts with the multi-domain Ran-binding protein in the microtubule-organising centre.

    Science.gov (United States)

    Menon, Rajesh P; Gibson, Toby J; Pastore, Annalisa

    2004-10-01

    Absence of the fragile X mental retardation protein (FMRP) causes fragile X syndrome, the most common form of hereditary mental retardation. FMRP is a mainly cytoplasmic protein thought to be involved in repression of translation, through a complex network of protein-protein and protein-RNA interactions. Most of the currently known protein partners of FMRP recognise the conserved N terminus of the protein. No interaction has yet been mapped to the highly charged, poorly conserved C terminus, so far thought to be involved in RNA recognition through an RGG motif. In the present study, we show that a two-hybrid bait containing residues 419-632 of human FMRP fishes out a protein that spans the sequence of the Ran-binding protein in the microtubule-organising centre (RanBPM/RanBP9). Specific interaction of RanBPM with FMRP was confirmed by in vivo and in vitro assays. In brain tissue sections, RanBPM is highly expressed in the neurons of cerebral cortex and the cerebellar purkinje cells, in a pattern similar to that described for FMRP. Sequence analysis shows that RanBPM is a multi-domain protein. The interaction with FMRP was mapped in a newly identified CRA motif present in the RanBPM C terminus. Our results suggest that the functional role of RanBPM binding is modulation of the RNA-binding properties of FMRP.

  10. The Compassionate Organisation

    DEFF Research Database (Denmark)

    Jørgensen, Poul Erik Flyvholm; Isaksson, Maria

    2015-01-01

    Structured Abstract: Purpose – This paper tests whether organisations in the public domain have embraced a corporate type of discourse, mirroring the private sector’s preferred orientation towards expertise, or whether they maintain their traditional discourse of goodwill towards the publics they...

  11. Organising integration

    DEFF Research Database (Denmark)

    Axelsson, Runo

    2013-01-01

    to large-scale bureaucratic structures of centralised control. In the 1980’s there was a period of decentralisation to smaller organisational units, which was followed in the 1990’s by an introduction of market mechanisms in accordance with the New Public Management. During this period, there has been......Background: In Sweden, as in many other countries, there has been a succession of trends in the organisation of health care and other welfare services. These trends have had different implications for the integration of services in the health and welfare system. Aims: One aim is to discuss...... the implications of different organisational trends for the integration of health and welfare services. Another aim is to introduce a Swedish model of financial coordination as a flexible way to organise integration. Organisational trends: In the 1960’s there was an expansion of health and welfare services leading...

  12. Inquiring Organisations

    OpenAIRE

    James Courtney; David Croasdell; David Paradice

    1998-01-01

    Churchman (1971) developed five archetypal models of inquiring systems in an effort to expand the field of management information systems along a philosophical path. Contemporary businesses can use the ideas developed by Churchman to become productive and efficient inquiring organisations. This paper explores the relationship between inquiry and learning in organisations and how information technology can be used to support the process of knowledge creation in the context of inquiring systems.

  13. Organisational Change

    DEFF Research Database (Denmark)

    Poulsen, Peter

    This Ph.D. research is carried out for the business unit at LEGO concerned with Internet shopping (e-business) called LEGO Direct. The research is concerned with the issues of organisational change and management. The research is partly sponsored by LEGO Company and Aalborg University The research...... understanding of organizational change and its processes both theoretical as well as empirical. In the search for interesting and relevant theories that would fulfill the goal of thesis, we learnt that the field of organisational change was complex and widely spread across lots of disciplines and paradigms......, ranging from areas such as engineering, psychology, management, and sociology. We also learnt that all of the theories were adding bits and pieces to our understanding of organisational change. During the search and selection, we found that it would be interesting to analyse what can be gained from...

  14. Barndommens organisering

    DEFF Research Database (Denmark)

    Barndommens Organisering undersøger og diskuterer dansk barndom som et samfundsmæssigt fænomen. Det er bogens mål at pege på en mangfoldighed af organiseringsprocesser, der på forskellig vis bidrager til at skabe barndommens rum, både de symbolske og de materielle. Bogens artikler er skrevet af...

  15. TEAM ORGANISERING

    DEFF Research Database (Denmark)

    Levisen, Vinie; Haugaard, Lena

    2004-01-01

    organisation som denne? Når teams i samtiden anses for at være en organisationsform, der fremmer organisatorisk læring, beror det på, at teamet antages at udgøre et ikke-hierarkisk arbejdsfællesskab, hvor erfaringer udveksles og problemer løses. Teamorganisering kan imidlertid udformes på mange forskellige...

  16. Organisational learning by way of organisational development

    DEFF Research Database (Denmark)

    Elkjær, Bente

    project contributes toorganisational learning. The point of departure is a municipality in Denmark workingtoward digitalising its administration. The conclusion is that the success of such aprocess very much depends on an organisation's ability to encompass severalunderstandings of organisational......In the paper, the idea is explored of organisational learning as the opening andclosure of organisational space for inquiry or reflective thinking, as a way toconstruct organisational learning as an object for research. This is done by asking thequestion of whether an organisational development...

  17. Organisational learning by way of organisational development

    OpenAIRE

    Elkjær, Bente

    2004-01-01

    In the paper, the idea is explored of organisational learning as the opening andclosure of organisational space for inquiry or reflective thinking, as a way toconstruct organisational learning as an object for research. This is done by asking thequestion of whether an organisational development project contributes toorganisational learning. The point of departure is a municipality in Denmark workingtoward digitalising its administration. The conclusion is that the success of such aprocess ver...

  18. Building a Learning Organisation

    OpenAIRE

    Tan, Gaik See

    2007-01-01

    According to Fortune magazine, the most successful corporation of the 1990s is those learning organisations who learn faster than their competitors. The concept of a learning organisation is becoming increasingly important in this knowledge-based economy. Continuos learning is essential in order for organisation tu sustain its competitive advantage.The concept of organisational learning and learning organisation emerged during the 1960s and 1970s. Learning organisation is a collective entity ...

  19. The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.

    Directory of Open Access Journals (Sweden)

    Audrey Costes

    Full Text Available We have investigated in vivo the role of the carboxy-terminal domain of the Bacillus subtilis Single-Stranded DNA Binding protein (SSB(Cter as a recruitment platform at active chromosomal forks for many proteins of the genome maintenance machineries. We probed this SSB(Cter interactome using GFP fusions and by Tap-tag and biochemical analysis. It includes at least 12 proteins. The interactome was previously shown to include PriA, RecG, and RecQ and extended in this study by addition of DnaE, SbcC, RarA, RecJ, RecO, XseA, Ung, YpbB, and YrrC. Targeting of YpbB to active forks appears to depend on RecS, a RecQ paralogue, with which it forms a stable complex. Most of these SSB partners are conserved in bacteria, while others, such as the essential DNA polymerase DnaE, YrrC, and the YpbB/RecS complex, appear to be specific to B. subtilis. SSB(Cter deletion has a moderate impact on B. subtilis cell growth. However, it markedly affects the efficiency of repair of damaged genomic DNA and arrested replication forks. ssbΔCter mutant cells appear deficient in RecA loading on ssDNA, explaining their inefficiency in triggering the SOS response upon exposure to genotoxic agents. Together, our findings show that the bacterial SSB(Cter acts as a DNA maintenance hub at active chromosomal forks that secures their propagation along the genome.

  20. The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.

    Science.gov (United States)

    Costes, Audrey; Lecointe, François; McGovern, Stephen; Quevillon-Cheruel, Sophie; Polard, Patrice

    2010-01-01

    We have investigated in vivo the role of the carboxy-terminal domain of the Bacillus subtilis Single-Stranded DNA Binding protein (SSB(Cter)) as a recruitment platform at active chromosomal forks for many proteins of the genome maintenance machineries. We probed this SSB(Cter) interactome using GFP fusions and by Tap-tag and biochemical analysis. It includes at least 12 proteins. The interactome was previously shown to include PriA, RecG, and RecQ and extended in this study by addition of DnaE, SbcC, RarA, RecJ, RecO, XseA, Ung, YpbB, and YrrC. Targeting of YpbB to active forks appears to depend on RecS, a RecQ paralogue, with which it forms a stable complex. Most of these SSB partners are conserved in bacteria, while others, such as the essential DNA polymerase DnaE, YrrC, and the YpbB/RecS complex, appear to be specific to B. subtilis. SSB(Cter) deletion has a moderate impact on B. subtilis cell growth. However, it markedly affects the efficiency of repair of damaged genomic DNA and arrested replication forks. ssbΔCter mutant cells appear deficient in RecA loading on ssDNA, explaining their inefficiency in triggering the SOS response upon exposure to genotoxic agents. Together, our findings show that the bacterial SSB(Cter) acts as a DNA maintenance hub at active chromosomal forks that secures their propagation along the genome. PMID:21170359

  1. A Polymer Model with Epigenetic Recolouring Reveals a Pathway for the de novo Establishment and 3D Organisation of Chromatin Domains

    CERN Document Server

    Michieletto, Davide; Marenduzzo, Davide

    2016-01-01

    One of the most important problems in development is how epigenetic domains can be first established, and then maintained, within cells. To address this question, we propose a framework which couples 3D chromatin folding dynamics, to a "recolouring" process modelling the writing of epigenetic marks. Because many intra-chromatin interactions are mediated by bridging proteins, we consider a "two-state" model with self-attractive interactions between two epigenetic marks which are alike (either active or inactive). This model displays a first-order-like transition between a swollen, epigenetically disordered, phase, and a compact, epigenetically coherent, chromatin globule. If the self-attraction strength exceeds a threshold, the chromatin dynamics becomes glassy, and the corresponding interaction network freezes. By modifying the epigenetic read-write process according to more biologically-inspired assumptions, our polymer model with recolouring recapitulates the ultrasensitive response of epigenetic switches t...

  2. Convention entre le Conseil fédéral de la Confédération suisse et le Gouvernement de la République française relative à l'extension en territoire français du domaine de l'Organisation européenne pour la recherche nucléaire

    CERN Document Server

    CERN. Geneva

    1965-01-01

    Convention entre le Conseil fédéral de la Confédération suisse et le Gouvernement de la République française relative à l'extension en territoire français du domaine de l'Organisation européenne pour la recherche nucléaire

  3. Beta-Globin Gene Regulation and Nuclear Organisation

    NARCIS (Netherlands)

    J.A. Kooren (Jurgen)

    2007-01-01

    textabstractAll genetic information required for the development and functioning of an organism is stored in billions of base pairs of deoxyribonucleic acid (or DNA). In eukaryotes, DNA is organised in large units called chromosomes that are located inside the cells nucleus. On these chromosomes res

  4. Organisational Learning: Theoretical Shortcomings and Practical Challenges

    Directory of Open Access Journals (Sweden)

    Jon Aarum Andersen

    2014-05-01

    Full Text Available This paper addresses two problems related to learning and the use of knowledge at work. The first problem is the theoretical shortcomings stemming from the controversy between three different concepts of ‘organisational learning.’ In order to enhance scholarship in this field the notion that organisations - as organisations - can learn need to be rejected for theoretical and empirical reasons. The metaphorical use of ‘organisational learning’ creates only confusion. Learning is a process and knowledge is the outcome of that process. It is argued that learning and knowledge is only related to individuals. Knowledge is thus the individual capability to draw distinctions, within a domain of action, based on an appreciation of context or theory. Consequently, knowledge becomes organisational when it is created, developed and transmitted to other individuals in the organisation. In a strict sense knowledge becomes organisational when employees use it and act based on generalisations due to the rules and procedures found in their organisation. The gravest problem is practical challenges due to the fact that the emphasis on learning, knowledge and competence of the working force do not materialize in the application of the knowledge acquired. It is evident that employees do not use their increased knowledge. However, we do not know why they do not use it. An enormous waste of money is spent on learning and knowledge in organisations which does not yield what is expected. How can managers act in order to enhance the application of increased knowledge possessed by the workforce?

  5. Amphipathic alpha-helices and putative cholesterol binding domains of the influenza virus matrix M1 protein are crucial for virion structure organisation.

    Science.gov (United States)

    Tsfasman, Tatyana; Kost, Vladimir; Markushin, Stanislav; Lotte, Vera; Koptiaeva, Irina; Bogacheva, Elena; Baratova, Ludmila; Radyukhin, Victor

    2015-12-01

    The influenza virus matrix M1 protein is an amphitropic membrane-associated protein, forming the matrix layer immediately beneath the virus raft membrane, thereby ensuring the proper structure of the influenza virion. The objective of this study was to elucidate M1 fine structural characteristics, which determine amphitropic properties and raft membrane activities of the protein, via 3D in silico modelling with subsequent mutational analysis. Computer simulations suggest the amphipathic nature of the M1 α-helices and the existence of putative cholesterol binding (CRAC) motifs on six amphipathic α-helices. Our finding explains for the first time many features of this protein, particularly the amphitropic properties and raft/cholesterol binding potential. To verify these results, we generated mutants of the A/WSN/33 strain via reverse genetics. The M1 mutations included F32Y in the CRAC of α-helix 2, W45Y and W45F in the CRAC of α-helix 3, Y100S in the CRAC of α-helix 6, M128A and M128S in the CRAC of α-helix 8 and a double L103I/L130I mutation in both a putative cholesterol consensus motif and the nuclear localisation signal. All mutations resulted in viruses with unusual filamentous morphology. Previous experimental data regarding the morphology of M1-gene mutant influenza viruses can now be explained in structural terms and are consistent with the pivotal role of the CRAC-domains and amphipathic α-helices in M1-lipid interactions.

  6. Progress in the domain of emissions tracking and environment radioactivity monitoring - Proceedings of the technical days organised by the SFRP Environment Section

    International Nuclear Information System (INIS)

    The Environment Section of the French Society of Radiation Protection (SFRP) organized a technical meeting on the progress made in the domain of emissions tracking and environment radioactivity monitoring. This document brings together the abstracts and the presentations (slides) of the different talks given at the meeting: 1 - Environment monitoring at the global, national and local scale: historical overview (Dominique CALMET, CEA); 2 - Evolution of radioactivity monitoring in the environment from 1960 to the present day (Guillaume MANIFICAT, IRSN); 3 - Euratom's legal framework (Zhana GENOVA, CTE); 4 - Main regulatory changes during the last decade (Nathalie REYNAL, ASN); 5 - Progress of standardization works on radioactive effluent emissions control and environment monitoring (Philippe BEGUINEL, BNEN); 6 - From operators' self-monitoring to ASN's inspections: a many components control system (Eric LE COZ, ASN); 7 - Control of effluents and emissions management at CEA Centres (Marianne CALVEZ, CEA); 8 - Liquid and gaseous effluents of ITER experimental facility: description and impacts (Pierre CORTES, IO); 9 - Effluents and emissions management strategy at AREVA NC La Hague facility (Stephane PUYDARRIEUX, AREVA); 10 - Radioactive effluents from nuclear facilities ongoing deconstruction: from dimensioning to real effluents (Benoit CLAVEL, EDF); 11 - Radionuclides decontamination process for liquid effluents using micro-algae at the laboratory scale (Corinne RIVASSEAU, CEA); 12 - Radioactive effluents from nuclear medicine services: management, monitoring and impact measurement methods (Nathalie TCHILIAN, ASN); 13 - Evolution history of effluents management and environment monitoring at the Solvay La Rochelle site (Thierry DELLOYE, SOLVAY); 14 - Different international approaches in effluents management and monitoring: example of French and German gaseous effluents - regulation, analyses, accounting rules (Jean-Jacques DIANA, ASN); 15 - Environment

  7. Researching enterprises between organisation and organising

    DEFF Research Database (Denmark)

    Elkjær, Bente; Brandi, Ulrik

    RESEARCHING ENTERPRISES BETWEEN ORGANISATION AND ORGANISING Ulrik Brandi & Bente Elkjaer, Department of Learning, University of Aarhus, Danish School of Education, Tuborgvej 164, 2400 Copenhagen NV, Denmark Short paper submission to the 26th European Group of Organization Studies Colloquium, Waves...... Dewey and Anselm Strauss. Based on a pragmatist theoretical framework we aim in this paper to propose concepts for organisation/organising that can handle process and structure, change and stability, order and disorder, history, present and future. We introduce the concept of a transactional relation...... philosophy and pragmatism followed by an elaboration of the transactional relationship between subject and world and the notion of social worlds. The final element in the paper is an empirical illustration - researching organisational learning in the midst of change that illustrates the rhythm...

  8. Administrativ organisation og ansvar

    DEFF Research Database (Denmark)

    Hilsted, Jannik C; Krogsgaard, Kim

    2003-01-01

    The paper describes the current organisation of clinical trials in Danish hospitals, with particular emphasis on the relationship between hospitals and the pharmaceutical industry. Legal responsibilities as well as mutual agreements on collaboration and organisation are described and discussed....

  9. Organisation og demokrati

    DEFF Research Database (Denmark)

    Fink, Jørgen Troelssøn

    1995-01-01

    the role of the business organisations of tyhe urban trades in the democratisation of Denmark 1830- 1915......the role of the business organisations of tyhe urban trades in the democratisation of Denmark 1830- 1915...

  10. Organising to Enable Innovation

    DEFF Research Database (Denmark)

    Brink, Tove

    2016-01-01

    . The findings reveal a continous organising process between individual/ team creativity and organisational structures/control to enable innovation at firm level. Organising provides a dynamic approach and contains the integrated reconstruction of creativity, structures and boundaries for enhanced balance......The purpose of this conceptual paper is to reveal how organising can enable innovation across organisational layers and organisational units. This approach calls for a cross-disciplinary literature review. The aim is to provide an integrated understanding of innovation in an organisational approach...... of explorative and exploitative learning in uncertain environments. Shedding light on the cross-disciplinary theories to organise innovation provides a contribution at the firm level to enable innovation....

  11. Organisational Structure & Change

    Science.gov (United States)

    National Centre for Vocational Education Research (NCVER), 2006

    2006-01-01

    Structural change is seen as a way to meet the challenges of the future that face many organisations. While some writers agree that broad-ranging structural change may not always transform an organisation or enhance its performance, others claim that innovation will be a major source of competitive advantage to organisations, particularly when…

  12. The peripheral chromosome scaffold, a novel structural component of mitotic chromosomes.

    Science.gov (United States)

    Sheval, Eugene V; Polyakov, Vladimir Y

    2008-06-01

    Using an original high-salt extraction protocol, we observed a novel chromosome substructure, referred to as the peripheral chromosome scaffold. This chromosome domain contained the perichromosomal layer proteins pKi-67, B23/nucleophosmin and fibrillarin, but no DNA fragments (i.e., the loop domain bases were not associated with the peripheral scaffold). Modern models of chromosome organization do not predict the existence of a peripheral chromosome scaffold domain, and thus our observations have conceptual implications for understanding chromosome architecture. PMID:18337132

  13. The process of organisational adaptation through innovations, and organisational adaptability

    OpenAIRE

    Tikka, Tommi

    2010-01-01

    This study is about the process of organisational adaptation and organisational adaptability. The study generates a theoretical framework about organisational adaptation behaviour and conditions that have influence on success of organisational adaptation. The research questions of the study are: How does an organisation adapt through innovations, and which conditions enhance or impede organisational adaptation through innovations? The data were gathered from five case organisations withi...

  14. ORGANISATIONAL CULTURE ANALYSIS MODEL

    OpenAIRE

    Mihaela Simona Maracine

    2012-01-01

    The studies and researches undertaken have demonstrated the importance of studying organisational culture because of the practical valences it presents and because it contributes to increasing the organisation’s performance. The analysis of the organisational culture’s dimensions allows observing human behaviour within the organisation and highlighting reality, identifying the strengths and also the weaknesses which have an impact on its functionality and development. In this paper, we try to...

  15. The Inner Centromere Protein (INCENP) Coil Is a Single α-Helix (SAH) Domain That Binds Directly to Microtubules and Is Important for Chromosome Passenger Complex (CPC) Localization and Function in Mitosis.

    Science.gov (United States)

    Samejima, Kumiko; Platani, Melpomeni; Wolny, Marcin; Ogawa, Hiromi; Vargiu, Giulia; Knight, Peter J; Peckham, Michelle; Earnshaw, William C

    2015-08-28

    The chromosome passenger complex (CPC) is a master regulator of mitosis. Inner centromere protein (INCENP) acts as a scaffold regulating CPC localization and activity. During early mitosis, the N-terminal region of INCENP forms a three-helix bundle with Survivin and Borealin, directing the CPC to the inner centromere where it plays essential roles in chromosome alignment and the spindle assembly checkpoint. The C-terminal IN box region of INCENP is responsible for binding and activating Aurora B kinase. The central region of INCENP has been proposed to comprise a coiled coil domain acting as a spacer between the N- and C-terminal domains that is involved in microtubule binding and regulation of the spindle checkpoint. Here we show that the central region (213 residues) of chicken INCENP is not a coiled coil but a ∼ 32-nm-long single α-helix (SAH) domain. The N-terminal half of this domain directly binds to microtubules in vitro. By analogy with previous studies of myosin 10, our data suggest that the INCENP SAH might stretch up to ∼ 80 nm under physiological forces. Thus, the INCENP SAH could act as a flexible "dog leash," allowing Aurora B to phosphorylate dynamic substrates localized in the outer kinetochore while at the same time being stably anchored to the heterochromatin of the inner centromere. Furthermore, by achieving this flexibility via an SAH domain, the CPC avoids a need for dimerization (required for coiled coil formation), which would greatly complicate regulation of the proximity-induced trans-phosphorylation that is critical for Aurora B activation.

  16. Organisational intelligence and distributed AI

    OpenAIRE

    Kirn, Stefan

    1995-01-01

    The analysis of this chapter starts from organisational theory, and from this it draws conclusions for the design, and possible organisational applications, of Distributed AI systems. We first review how the concept of organisations has emerged from non-organised black-box entities to so-called computerised organisations. Within this context, organisational researchers have started to redesign their models of intelligent organisations with respect to the availability of advanced computing tec...

  17. Designing comprehensible self-organising systems

    NARCIS (Netherlands)

    Höning, N.F.; La Poutré, J.A.

    2010-01-01

    Self-organising systems are a popular engineering concept for designing decentralised autonomic computing systems. They are able to find solutions in complex and versatile problem domains, but as they capture more complexity in their own design, they are becoming less and less comprehensible to thei

  18. Kommunikation skaber din organisation

    DEFF Research Database (Denmark)

    Hansen, Heidi

    KOMMUNIKATION skaber din ORGANISATION tager udgangspunkt i en narrativ tilgang til kommunikation, hvor organisationen skabes i mødet mellem ledere, medarbejdere, organisation og omverden. Historier hjælper os med at skabe mening, og er derfor vigtige både som et udviklingsværktøj i organisationen...

  19. Organisational Structures & Considerations

    NARCIS (Netherlands)

    Luiijf, H.A.M.; Healey, J.

    2012-01-01

    The purpose of this section is to review specific types of national cyber security (NCS) areas (also called ‘mandates’) and examine the organisational and collaborative models associated with them. Before discussing the wide variety of organisational structures at the national and international leve

  20. Introducing organisational heritage: Linking corporate heritage, organisational identity, and organisational memory

    OpenAIRE

    Balmer, JMT; Burghausen, M

    2015-01-01

    In this article we formally introduce and explicate the organisational heritage notion. The authors conclude organisational heritage can be designated in three broad ways as: (1) organisational heritage identity as the perceived and reminisced omni-temporal traits – both formal/normative and utilitarian/societal – of organisational members’ work organisation; (2) organisational heritage identification as organisational members’ identification/self-categorisation vis-à-vis these perceived and ...

  1. Self-organising software

    CERN Document Server

    Serugendo, Giovanna Di Marzo; Karageorgos, Anthony

    2011-01-01

    Self-organisation, self-regulation, self-repair and self-maintenance are promising conceptual approaches for dealing with complex distributed interactive software and information-handling systems. Self-organising applications dynamically change their functionality and structure without direct user intervention, responding to changes in requirements and the environment. This is the first book to offer an integrated view of self-organisation technologies applied to distributed systems, particularly focusing on multiagent systems. The editors developed this integrated book with three aims: to exp

  2. Researching enterprises between organisation and organising

    OpenAIRE

    Elkjær, Bente; Brandi, Ulrik

    2010-01-01

    RESEARCHING ENTERPRISES BETWEEN ORGANISATION AND ORGANISINGUlrik Brandi & Bente Elkjaer, Department of Learning, University of Aarhus, Danish School of Education, Tuborgvej 164, 2400 Copenhagen NV, DenmarkShort paper submission to the 26th European Group of Organization Studies Colloquium, Waves of Globalization: Repetition and difference in organizing over time and space. June 30 - July 3 2010, Lisbon, Portugal.Sub-theme 16:  Investigating Organization as Becoming in a World on the Move ...

  3. Organisational Theatre and Polyphony

    DEFF Research Database (Denmark)

    Matula, Linda; Badham, Richard; Meisiek, Stefan

    of the organisational theatre event. It focuses in particular on the alignments and clashes between the different human resource voices in defining the ‘surface’ formal purpose for the intervention and the embeddedness of such interactions and negotiations in ‘deeper’ cultural and social conditions. The paper provides......This paper details the conditions leading up to and influencing an organisational theatre intervention as part of an organisational change program at a newly established cancer clinic. The paper explores the social and political interactions and negotiations shaping the structure and conditions...... the first in-depth longitudinal study of shaping and negotiation of an organisational theatre event and the ways in which it is influenced by a polyphonic multivocality and takes the form of selective and partial forms of harmonious expression in establishing meaningful cooperation. The paper reveals...

  4. Leveraging organisational cultural capital

    Directory of Open Access Journals (Sweden)

    R Scheel

    2007-01-01

    Full Text Available Organisational culture discourse mandates a linear approach of diagnosis, measurement and gap analysis as standard practice in relation to most culture change initiatives. Therefore, a problem solving framework geared toward “fixing�? and/or realigning an organisation’s culture is usually prescribed. The traditional problem solving model seeks to identify gaps between current and desired organisational cultural states, inhibiting the discovery of an organisation’s unique values and strengths, namely its cultural capital. In pursuit of discovering and leveraging organisational cultural capital, a descriptive case study is used to show how an Appreciative Inquiry process can rejuvenate the spirit of an organisation as a system-wide inquiry mobilises a workforce toward a shared vision.

  5. Organisations in Innovation Systems

    DEFF Research Database (Denmark)

    Borrás, Susana

    Organisations are crucial elements in an innovation system. Yet, their role is so ubiquitous that it is difficult to grasp and to examine from the perspective of public policy. Besides, links between the literature at firm and system levels on the one hand, and public policy and governance studies...... on the other, are still scarce. The purpose of this paper is to define the conceptual background of innovation policy in relation to the role of organisations in general, and entrepreneurship and intrapreneurship in particular. In so doing, this paper aims at making three contributions. Firstly......, it distinguishes between different types of organisations in the innovation system, a crucial topic in understanding innovation dynamics and blurring borders. Secondly, it identifies the organisation-related bottlenecks in the innovation system, and examines the policy instruments to solve them. Thirdly...

  6. Strategy Development in Organisations

    DEFF Research Database (Denmark)

    Sørensen, Lene

    1999-01-01

    There exist certain ambiguities with the converging fields of information technology and organisational strategy development. The term "IT strategy" has evolved and reflects in some respects this confusion. This paper discusses some of the ambiguities and difficulties of the term "IT strategy......" as used in practice and literature. Emphasis is put on how the term is related to the problem, the organisation, the strategy process and the practical way of methodologically developing the strategy. Finally, alternative strategy developing perspectives are presented....

  7. The Organisation of Disappointment

    OpenAIRE

    Clancy, Annette

    2012-01-01

    The aim of this study is to explore the emotion of disappointment in organisations and to develop a new line of theorising inspired by psychodynamic theory. The current literature casts disappointment as a negative emotion undermining morale, depressing expectations and justifying inaction and inertia. This only captures part of the complexity of disappointment and leaves unexplored both its impact on the organisation and its potential creativity. The study presents a theoretical framework de...

  8. Organisational learning in professional discourses.

    Directory of Open Access Journals (Sweden)

    Martin Sedláček

    2012-06-01

    Full Text Available Authors of the paper discuss the concept of organisational learning. Theyrefer to a wide range of definitions and concepts, point out some differences from related or analogous concepts used, such as knowledge management and learning organisation. They note the discussions that relate to key topics. These discussions concern levels at which organisational learning can take place, the effect on behaviour organisational performance. The following section of this article presents organisational learning as a process and describes the stages of organisational learning. The purpose of the article is to provide an overview of efforts to define organisational learning, including criticism of the results of this effort. The last partis devoted to selected theories and empirical findings on organisational learning atschool. The authors stress the importance of organisational learning for schools asspecific organisations and express request to examine the processes of organisational learning in this context.

  9. The organisational diagnoses of a distribution organisation / Lourence Badenhorst Alblas

    OpenAIRE

    Alblas, Lourence Badenhorst

    2002-01-01

    During the past three decades the economic performances of South Africa organisations were poor. This poor performance forced a lot of South African organisations to look at the way they do business. A method to analyse the way business is conducted through an Organisation Diagnose. Organisation Diagnose is the process of using concepts and methods from the social and behavioural sciences to assess the current state of an entire organisation and to find ways to improve th...

  10. Organisational learning in professional discourses.

    OpenAIRE

    Martin Sedláček; Petr Novotný; Lenka Hloušková; Milan Pol; Bohumíra Lazarová

    2012-01-01

    Authors of the paper discuss the concept of organisational learning. Theyrefer to a wide range of definitions and concepts, point out some differences from related or analogous concepts used, such as knowledge management and learning organisation. They note the discussions that relate to key topics. These discussions concern levels at which organisational learning can take place, the effect on behaviour organisational performance. The following section of this article presents organisational ...

  11. Strategising through organising

    DEFF Research Database (Denmark)

    Larsen, Mette Vinther

    and sensemaking point of view. It argues that actors’ strategising is closely connected to their organising. Maybe strategising and organising co-constitute each other? It is a perspective that looks at strategy as emergent wayfinding more than planned navigating. In the attempts to make sense...... of and operationalise a strategy, maybe actors do not follow a pre-defined map, but instead figure out the way as they go? Maybe actors go in ways that they relationally believe are the ‘right ones’ and in ways that make sense to them? There are, however, many actors in an organisation and, therefore, also many ways......This thesis is about strategy. It is about what happens after the strategy leaves the drawing board and actors try to make sense of it and figure out how to act. There are many perspectives on strategy. This is another one. A perspective that tries to work with strategy from an everyday, relational...

  12. Luhmann og Organisation

    DEFF Research Database (Denmark)

    Paulsen, Michael

    LUHMANN OG ORGANISATION Organisationsstudier spiller en særlig rolle for den tyske sociolog Niklas Luhmann (1927-1998) idet han begriber organisationer som det mest afgørende socialsystem i det moderne samfund. Kun organisati-oner kan træffe samfundsmæssige afgørelser. Faktisk begyndte Luhmann som...... organisationsteoretiker, og flere af hans tidlige værker regnes nu som milepæle indenfor organisations-studier. I 2000 udkom post humt hans sidste store organisationsteoretiske værk Organisation und Entscheidung. I Luhmanns almene systemteori trækker han kraftigt på sit kendskab til organisationer. Begribelsen af...... organisationer i sammenhæng med det omgivende samfund, formidlet med Luhmanns kommunikations-teoretiske begrebsapparat giver hans bidrag til organisationsteori en enestående analytisk styrke. Omdrejningspunktet for Luhmann har været at forstå, hvordan organisationer kan lukke sig omkring deres egne beslutninger...

  13. Between Organisation and Architecture

    DEFF Research Database (Denmark)

    Stang Våland, Marianne

    2011-01-01

    . In this process, the open-office layout was introduced as a premediated design condition, a format that the users initially resisted. The paper discusses how end-user participation as a method and spatial design as a perspective may inform change in organisations. Although the users' resistance toward the open......This paper contributes to our sparse knowledge on the relationship between organisational and architectural design. It is based on an ethnographic study of the process of designing a municipality town hall, in which end-user participation constituted an integrated part of the design process...

  14. Den kommunikerende organisation

    DEFF Research Database (Denmark)

    Tække, Jesper

    2006-01-01

    samtidig tilbydes optimale muligheder for at få opfyldt individuelle behov og karrieremuligheder. For at en organisation skal kunne korrigere sig i forhold til de krav den konfronteres med, må der kunne fremstilles et adækvat billede af den. Det vil sige at dens faktiske kommunikationsprocesser må kunne...... drages frem i lyset på en måde så hensigtsmæssigheder og uhensigtsmæssigheder kan iagttages og selv blive objekt for reflekteret organisationskommunikation. Dette paper giver et udkast til en analysestrategi, hvormed der kan tegnes et billede af den enkelte organisation ud fra hvordan dens enkelte og...

  15. Exploring the Impact that Organisational Culture and Structures have on Knowledge Management Initiatives

    OpenAIRE

    Digan, Gavin

    2015-01-01

    The project builds on research in the domain of knowledge management, with a literature review covering several aspects of the domain. There is particular emphasis on knowledge management implementations within organisations. Several researchers in the area offer methodologies or strategies for organisations to adopt, when implementing knowledge management initiatives. These options are covered at length in the literature review along with real world case studies on organisations that have im...

  16. Organisational Learning with Technology

    DEFF Research Database (Denmark)

    Hundebøl, Jesper

    2008-01-01

    in practice. I will discuss these cases with a view to understand the implications for organising, learning and knowing in the design phases of a construction projects. Before this, however, I will present in detail the research question and how I intend to deal with it methodologically. I will outline...

  17. Four archetypal networked organisations

    NARCIS (Netherlands)

    Treurniet, W.; Buul, K. van

    2015-01-01

    Complex and multifaceted problems such as emergencies typically require coordinated effort by a network of different organisations. These networks typically rely less on formal hierarchical structures and instead have a stronger focus on allowing the dynamics to emerge in the process of collaboratio

  18. The national emergency organisation

    International Nuclear Information System (INIS)

    In any emergency situation, the organisation of the response in France is based on predetermined emergency response plans. These plans define worst-case accident scenarios in terms of safety and the measures necessary to control the accident and to protect the personnel on the sites as well as the general public. (authors)

  19. Cultures et organisations

    NARCIS (Netherlands)

    Hofstede, G.; Hofstede, G.J.; Minkov, M.

    2010-01-01

    La coopération interculturelle, une question de survie Véritable atlas des valeurs culturelles, paru en 18 langues, Cultures et organisations est le fruit de plus de 40 ans de recherches menées dans plus de 100 pays. Il est aujourd'hui le livre de référence des chercheurs, universitaires et professi

  20. Den Legende Organisation

    DEFF Research Database (Denmark)

    Thorsted, Ann Charlotte

    Bogen beskriver, hvordan to vidt forskellige organisationer - den store internationale virksomhed LEGO og den mindre nonprofit organisation SUS - har bragt legen i spil. Ann Charlotte Thorsted viser i bogen, hvordan leg kan understøtte innovation, læring, kommunikation, samarbejde og det gode...

  1. Polymer models of chromosome (re)organization

    Science.gov (United States)

    Mirny, Leonid

    Chromosome Conformation Capture technique (Hi-C) provides comprehensive information about frequencies of spatial interactions between genomic loci. Inferring 3D organization of chromosomes from these data is a challenging biophysical problem. We develop a top-down approach to biophysical modeling of chromosomes. Starting with a minimal set of biologically motivated interactions we build ensembles of polymer conformations that can reproduce major features observed in Hi-C experiments. I will present our work on modeling organization of human metaphase and interphase chromosomes. Our works suggests that active processes of loop extrusion can be a universal mechanism responsible for formation of domains in interphase and chromosome compaction in metaphase.

  2. Organisational Memetics?: Organisational Learning as a Selection Process

    OpenAIRE

    Price, Ilfryn

    1995-01-01

    Companies are not only systems created and controlled by those who manage them but also self-organising entities that evolve through learning. Whereas an organism is a creation of natural replicators, genes, an organisation can be seen as a product of an alternative replicator, the meme or mental model, acting, like a gene, to preserve itself in an Evolutionary Stable System. The result is an organisation which self organises around a set of unspoken and unwritten rules and assumptions. ...

  3. The Impact of Organisational Learning on Organisational Performance

    OpenAIRE

    Anna Zgrzywa-Ziemak

    2015-01-01

    Purpose: The aim of this article is to analyse the theoretical views and results of empirical research concerning the relation between organisational learning (OL) and organisational performance (OP). Methodology: The study was carried out through extensive literature research, including relevant literature review from databases such as ProQuest, Elsevier, Emerald and EBSCO (the phrases: “organisational learning”, “learning organisation” and “organisational performance” were searched in th...

  4. MEMO Organisation Modelling Language (1): Focus on organisational structure

    OpenAIRE

    Frank, Ulrich

    2011-01-01

    Organisation models are at the core of enterprise model, since they represent key aspects of a company's action system. Within MEMO, the Organisation Modelling Language (OrgML) supports the construction of organisation models. They can be divided into two main abstractions: a static abstraction is focusing on the structure of an organisation that reflects the division of labour with respect to static responsibilities and a dynamic abstraction that is focusing on models of business processes. ...

  5. World Organisation for Animal Health

    Science.gov (United States)

    World Organisation for Animal Health Home About us Presentation Director general office Biography Photos Strategic plan Our ... Food safety and animal welfare History General organisation World Assembly Council Headquarters OIE Regional Representations OIE Regional ...

  6. Learning and change in organisations

    DEFF Research Database (Denmark)

    Chemi, Tatiana

    2013-01-01

    Review of: Ian W. King & Jonathan Vickery (Eds.), Experiencing Organisations. New Aesthetic perspectives, Faringdon, Libri Publishing, 2013.......Review of: Ian W. King & Jonathan Vickery (Eds.), Experiencing Organisations. New Aesthetic perspectives, Faringdon, Libri Publishing, 2013....

  7. Virtual corporations, enterprise and organisation

    OpenAIRE

    Carmen RÃDUT

    2009-01-01

    Virtual organisation is a strategic paradigm that is centred on the use of information and ICT to create value. Virtual organisation is presented as a metamanagement strategy that has application in all value oriented organisations. Within the concept of Virtual organisation, the business model is an ICT based construct that bridges and integrates enterprise strategic and operational concerns. Firms try to ameliorate the impacts of risk and product complexity by forming alliances and partners...

  8. EVALUATION OF THE PROJECT MANAGEMENT ORGANISATIONAL ABILITY OF PUBLIC ORGANISATIONS

    Directory of Open Access Journals (Sweden)

    Florescu Margareta

    2012-12-01

    Full Text Available The quality of the results of a project or a public programme, as well as the quality of project management consist in management process performance.This analysis tool promotes the idea of initiating a new organisational/functional policy – organisational tools regarding project management, a new standard concerning the complexity of the project and the associated risk, as well as a new standard concerning the project management organisational/functional ability. Political decision makers, public organisation managers, but project managers as well are the main people in charge of elaborating and taking responsibility for these standards and policies within the organisations they manage and represent.

  9. Virtualness : a new organisational dimension

    NARCIS (Netherlands)

    Bosch-Sijtsema, P.M.

    2003-01-01

    In current literature a new organisational form is presented, the virtual organisation (VO). ms organisational form is a co-operation between a number of companies who combine their strengths to develop a new service or product. The current VO literature is ambiguous about the definition and charact

  10. Customer Oriented Organisations

    OpenAIRE

    Pau Adriana

    2011-01-01

    Customer orientation is a complex, multidimensional concept, that includes dimensions like: motivation of an employee for meeting the customers’ requirements, abilities necessary for an employee to satisfy the customer’s needs, freedom of the employee to make decisions aimed at meeting the customer’s needs and desires. Customer oriented organisations offer services with a high level of quality that ensures the customer’s satisfaction.

  11. Managing a Voluntary Organisation

    OpenAIRE

    Dk. Hjh. Siti Fatimah - Pg. Hj. Petra

    2014-01-01

    This paper reveals the experience of a young member of a non-profitable organisation in managing a team of professionals in Brunei Darussalam. In a team, the experience of managing has not been as scary as expected; positions taken, however, must be firm and effective. Being a leader of the contingency approach type, actions and responses are based on circumstances. Marketing and managing changes need to be pursued as a new leader of a voluntary organization. Careful considerations and risks ...

  12. Droit des organisations internationales

    CERN Document Server

    Sorel, Jean-Marc; Ndior, Valère

    2013-01-01

    Cet ouvrage collectif offre aux enseignants et chercheurs en droit international, aux praticiens et aux étudiants, une analyse actualisée du droit des organisations internationales. Il dresse en cinq parties un tableau, illustré par des exemples variés, des problématiques que soulève le phénomène polymorphe d institutionnalisation de la société internationale. La première partie est consacrée au phénomène des « organisations internationales », sous l angle à la fois de l institutionnalisation progressive des relations internationales et de la difficulté à cerner une catégorie unifiée. La deuxième partie rend compte de la création, de la disparition et des mutations des organisations internationales, ici envisagées comme systèmes institutionnels et ordres juridiques dérivés. La troisième partie analyse l autonomie que l acquisition de la personnalité juridique et de privilèges et immunités, un organe administratif intégré, un personnel ou un budget propres confèrent aux organi...

  13. Ten Australian ICU nurses' perceptions of organisational restructuring.

    Science.gov (United States)

    Wynne, Rochelle

    2004-02-01

    The Australian healthcare system underwent radical reform in the 1990s as economic rationalist policies were embraced. As a result, there was significant organisational restructuring within hospitals. Traditional indicators, such as nursing absenteeism and attrition, increase during times of organisational change. Despite this, nurses' views of healthcare reform are under-represented in the literature and little is known about the impact of organisational restructuring on perceived performance. This study investigated the perceived impact of organisational restructuring on a group of intensive care unit (ICU) nurses' workplace performance. It employed a qualitative approach to collect data from a purposive sample of clinical nurses. The primary method of data collection was semi-structured interviews. Content analysis generated three categories of data. Participants identified constant pressure, inadequate communication and organisational components of restructuring within the hospital as issues that had a significant impact on their workplace performance. They perceived organisational restructuring was poorly communicated, and this resulted in an environment of constant pressure. Organisational components of restructuring included the subcategories of specialised service provision and an alternative administrative structure that had both positive and negative ramifications for performance. To date, there has been little investigation of nurses' perceptions of organisational restructure or the impact this type of change has in the clinical domain. Participants in this study believed reorganisation was detrimental to quality care delivery in intensive care, as a result of fiscal constraint, inadequate communication and pressure that influenced their workplace performance.

  14. Ten Australian ICU nurses' perceptions of organisational restructuring.

    Science.gov (United States)

    Wynne, Rochelle

    2004-02-01

    The Australian healthcare system underwent radical reform in the 1990s as economic rationalist policies were embraced. As a result, there was significant organisational restructuring within hospitals. Traditional indicators, such as nursing absenteeism and attrition, increase during times of organisational change. Despite this, nurses' views of healthcare reform are under-represented in the literature and little is known about the impact of organisational restructuring on perceived performance. This study investigated the perceived impact of organisational restructuring on a group of intensive care unit (ICU) nurses' workplace performance. It employed a qualitative approach to collect data from a purposive sample of clinical nurses. The primary method of data collection was semi-structured interviews. Content analysis generated three categories of data. Participants identified constant pressure, inadequate communication and organisational components of restructuring within the hospital as issues that had a significant impact on their workplace performance. They perceived organisational restructuring was poorly communicated, and this resulted in an environment of constant pressure. Organisational components of restructuring included the subcategories of specialised service provision and an alternative administrative structure that had both positive and negative ramifications for performance. To date, there has been little investigation of nurses' perceptions of organisational restructure or the impact this type of change has in the clinical domain. Participants in this study believed reorganisation was detrimental to quality care delivery in intensive care, as a result of fiscal constraint, inadequate communication and pressure that influenced their workplace performance. PMID:15011993

  15. Virtual corporations, enterprise and organisation

    Directory of Open Access Journals (Sweden)

    Carmen RÃDUT

    2009-06-01

    Full Text Available Virtual organisation is a strategic paradigm that is centred on the use of information and ICT to create value. Virtual organisation is presented as a metamanagement strategy that has application in all value oriented organisations. Within the concept of Virtual organisation, the business model is an ICT based construct that bridges and integrates enterprise strategic and operational concerns. Firms try to ameliorate the impacts of risk and product complexity by forming alliances and partnerships with others to spread the risk of new products and new ventures and to increase organisational competence. The result is a networked virtual organization.

  16. TEDx Organisers meet at CERN

    CERN Multimedia

    Abha Eli Phoboo

    2013-01-01

    CERN hosted the second TEDx European Organisers meeting last week with around 80 organisers attending from all over Europe. They were given an introduction to CERN and a tour of the LHC experiments.   The participants of the TEDx European Organisers meeting held at CERN last week. Among the attendees was Bruno Giussani, European director of TED, who delivered the welcome address. The TEDx European organisers shared their experiences in workshops and brainstormed about how to work on different aspects of organising a TEDx event, and about improving the relationship between TEDx and TED. “The goal of this meeting was for veteran TEDx organisers to help younger ones, help each other, bring the community together and have better quality events,” said Claudia Marcelloni, head of TEDxCERN. TEDx is an independently organised TED (Technology, Entertainment, Design) talk event, which has grown exponentially all over the world. There are hundreds of TEDx events every day and it n...

  17. Stable Chromosome Condensation Revealed by Chromosome Conformation Capture.

    Science.gov (United States)

    Eagen, Kyle P; Hartl, Tom A; Kornberg, Roger D

    2015-11-01

    Chemical cross-linking and DNA sequencing have revealed regions of intra-chromosomal interaction, referred to as topologically associating domains (TADs), interspersed with regions of little or no interaction, in interphase nuclei. We find that TADs and the regions between them correspond with the bands and interbands of polytene chromosomes of Drosophila. We further establish the conservation of TADs between polytene and diploid cells of Drosophila. From direct measurements on light micrographs of polytene chromosomes, we then deduce the states of chromatin folding in the diploid cell nucleus. Two states of folding, fully extended fibers containing regulatory regions and promoters, and fibers condensed up to 10-fold containing coding regions of active genes, constitute the euchromatin of the nuclear interior. Chromatin fibers condensed up to 30-fold, containing coding regions of inactive genes, represent the heterochromatin of the nuclear periphery. A convergence of molecular analysis with direct observation thus reveals the architecture of interphase chromosomes. PMID:26544940

  18. Intergovernmental organisation activities and Multilateral agreements

    International Nuclear Information System (INIS)

    This section summarises the activities of Intergovernmental organisations and the status of Multilateral agreements on December 1, 2011: 1 - Intergovernmental organisation activities: European Atomic Energy Community: Adopted legislative instruments, Reports, Meetings; International Atomic Energy Agency: IAEA Action Plan on Nuclear Safety, Non-binding instrument on the transboundary movement of scrap metal, 55. IAEA General Conference, Basic Safety Standards, Nuclear Law Institute; OECD Nuclear Energy Agency: Basic Safety Standards, International Nuclear Law Essentials, International School of Nuclear Law, New members, Russian Federation request for membership; 2 - Multilateral agreements: Status of conventions in the nuclear energy domain on December 1, 2011; Status of conventions in the environmental protection/evaluation impacting the nuclear energy use on December 1, 2011; participation of OECD Member States to nuclear energy and environment protection/evaluation related treaties/conventions

  19. Kommunikation skaber din organisation

    DEFF Research Database (Denmark)

    Hansen, Heidi

    Kommunikation er med til at skabe vores sociale verdener - og er dermed essentiel i forhold til at skabe virksomhedens image udadtil og den organisatoriske virkeligehd indadtil. Bogen tager udgangspunkt i en semiotisk og narrativ tilgang til kommunikation, hvor organisationen konstant (gen-)skabe......-)skabes genenm interaktioner mellem ledere og medarbejdere og mellem organisation og omverden. Identitet er et nøgleberegb i denne forståelse. Historier hjælper os med at skabe mening og er derfor vigtige som organisationsudviklings- og positioneringsværktøj....

  20. Organisational Learning with Technology

    DEFF Research Database (Denmark)

    Hundebøl, Jesper

    2008-01-01

    Based on multi-site ethno-methodological field studies in the Danish construction industry this paper examines the relational effects of 3D object-based modelling. In describing how that technology is being introduced, shaped and enacted, how it associates with, mediates and translates existing...... practices, I discuss how it has effects for work methods and routines in an (inter-)organisational setting, namely that of architects and consulting engineers. The technology is introduced in the practices in question, in part because of a program referred to as Det Digitale Byggeri (Digital Construction...

  1. Contemporary Network Organisations in Slovenia

    Directory of Open Access Journals (Sweden)

    Jure Kovač

    2014-11-01

    Full Text Available Modern organisations are confronted with enormous challenges. The need to continuously adapt to changing environments represents a main challenge for modern organisations. In order to adapt to the requirements of modern environments more easily and more rapidly, organisations become connected into networks. A network organisation is fast becoming a favourite form of the modern organisation. On the basis of an analysis of members in the field of network organisations, this contribution presents the dimensions and definitions of network organisations. In the follow-up, the starting point for a theoretical explanation of network organisations and their different existing forms is presented. The emphasis of the empirical part of the contribution focuses on presenting an analysis of forms of network organisations that are present in Slovenia from the perspective of their shape, development and actual state. Based on an analysis of relevant documentation, it may be concluded that it was the institutional environment which initiated and directed the start-up processes that led to the establishment of contemporary network organisations within Slovenia.

  2. Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretin receptor superfamily with an unusual extracellular domain

    Energy Technology Data Exchange (ETDEWEB)

    Hamann, J. [Univ. of Amsterdam (Netherlands)]|[Max Planck Society, Berlin-Buch (Germany); Hamann, D.; Lier, R.A.W. [Univ. of Amsterdam (Netherlands)] [and others

    1995-08-15

    CD97 is a monomeric glycoprotein of 75 to 85 kDa that is induced rapidly on the surface of most leukocytes upon activation. We herein report the isolation of a cDNA encoding human CD97 by expression cloning in COS cells. The 3-kb cDNA clone encodes a mature polypeptide chain of 722 amino acids with a predicted molecular mass of 79 kDa. Within the C-terminal part of the protein, a region with seven hydrophobic segments was identified, suggesting that CD97 is a seven-span transmembrane molecule. Sequence comparison indicates that CD97 is the first leukocyte Ag in a recently described superfamily that includes the receptors for secretin, calcitonin, and other mammalian and insect peptide hormones. Different from these receptors, CD97 has an extended extracellular region of 433 amino acids that possesses three N-terminal epidermal growth factor-like domains, two of them with a calcium-binding site, and single Arg-Gly-Asp (RGD) motif. The existence of structural elements characteristic for extracellular matrix proteins in a seven-span transmembrane molecule makes CD97 a receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. The gene encoding CD97 is localized on chromosome 19 (19p13.12-13.2).

  3. EVALUATION OF THE PROJECT MANAGEMENT ORGANISATIONAL ABILITY OF PUBLIC ORGANISATIONS

    OpenAIRE

    Florescu Margareta

    2012-01-01

    The quality of the results of a project or a public programme, as well as the quality of project management consist in management process performance.This analysis tool promotes the idea of initiating a new organisational/functional policy – organisational tools regarding project management, a new standard concerning the complexity of the project and the associated risk, as well as a new standard concerning the project management organisational/functional ability. Political decision makers,...

  4. Organisational Self - Evaluation as a Possible Tool of Organisational Analysis

    OpenAIRE

    Mariann Veresné Somosi

    2004-01-01

    The clue of enduring success of companies / institutes is the ability to recognise new challenges betimes and to react them quickly and flexible. The management however does not dispose of the appropriate tools and methodological knowledge in cases of complex and complicated organisational forming to map fields in critical situations. During this presentation, I examine one of the possible systems of goals and fields of organisational analysis with the help of the organisational analysis proc...

  5. Organisational Learning - A Global View

    International Nuclear Information System (INIS)

    This paper will discuss the concept of organisational learning and its application to NPP. The concept outlined in this paper substantially extends the operating experience feedback process currently practiced in NPP. Organisational learning has emerged from economics and business administration where measures related to this concept have been applied to increase economic efficiency and return. Such organisations are designed to achieve specific objectives by using specific means and resources. For these organisations, organisational learning is of utmost importance especially in changing environments, because it drives the systematic and continuous adaptation and self-improvement of an organisation in order to better carry out its missions and to better fulfil the economic, legal, political, scientific, social, and technological requirements of the environment. In the nuclear industry, besides design nuclear safety is guaranteed by an efficient safety management. Safety management includes the continuous monitoring, evaluation, and improvement of nuclear safety. Organisational learning is an essential element of safety management, and safety management is to be considered as a particular framework for organisational learning. To be efficient, organisational learning, like safety management, has to rely on core values of a good safety culture like e.g. questioning attitude, and openness to criticism. Chapter 2 of the paper elaborates on the definition of organisational learning and its main elements. Chapter 3 discusses the current status regarding the organisational learning system in German NPP. Chapter 4 presents concept and implementation status of an organisational learning system developed by the Unterweser NPP and GRS. The paper concludes with lessons learned up to now

  6. Research into Chinese media organisations

    OpenAIRE

    Xin, Xin

    2015-01-01

    This article examines the impact of media marketisation in China upon organisational structure and journalistic practices of Chinese news organisations. It aims to assess to what extent market factors have weakened the centralised control over local journalistic practices in the last two and a half decades. This paper focuses on Xinhua Shanghai Bureau, a local division within a central news organisation, Xinhua News Agency, which is headquartered in Beijing and operates nationwide. This artic...

  7. Feminine leadership and organisational culture

    OpenAIRE

    Andreea-Simona Saseanu; Sorin-George Toma; Paul Marinescu

    2014-01-01

    People in general, leaders especially, are influenced by the organisational culture and the other way around. Organisational culture represents a determining factor regarding the display of leadership, since these two processes create each other, adding value and consistency to one another. Moreover, organisational culture can be created and developed in a fluctuant business environment, in which the external factors influence its progress. The development of society has incessantly been emph...

  8. Chromosome Microarray.

    Science.gov (United States)

    Anderson, Sharon

    2016-01-01

    Over the last half century, knowledge about genetics, genetic testing, and its complexity has flourished. Completion of the Human Genome Project provided a foundation upon which the accuracy of genetics, genomics, and integration of bioinformatics knowledge and testing has grown exponentially. What is lagging, however, are efforts to reach and engage nurses about this rapidly changing field. The purpose of this article is to familiarize nurses with several frequently ordered genetic tests including chromosomes and fluorescence in situ hybridization followed by a comprehensive review of chromosome microarray. It shares the complexity of microarray including how testing is performed and results analyzed. A case report demonstrates how this technology is applied in clinical practice and reveals benefits and limitations of this scientific and bioinformatics genetic technology. Clinical implications for maternal-child nurses across practice levels are discussed. PMID:27276104

  9. Youth organisations as learning organisations: Exploring special contributions and challenges

    NARCIS (Netherlands)

    Felice, M.C. del; Solheim, L.

    2011-01-01

    Youth and youth organisations are becoming valuable development partners, but little knowledge about their characteristics as learning organisations exists. This article presents perceptions of youth workers on this topic. These were gathered via an online survey and through research done by a youth

  10. Organisational Learning and Organisational Memory for SMS and FRMS

    NARCIS (Netherlands)

    Koornneef, F.; Stewart, S.; Akselsson, R.; Ward, M.

    2009-01-01

    Chapter 1: Organisational Learning and Organisational Memory for SMS and FRMS The European Commission HILAS project (Human Integration into theLifecycle of Aviation Systems - a project supported by the European Commission’s 6th Framework between 2005-2009) was focused on using human factors knowled

  11. Organisational commitment and turnover intentions in humanitarian organisations

    NARCIS (Netherlands)

    Wijnmaalen, Julia; Heyse, Liesbet; Voordijk, Hans

    2016-01-01

    Unwanted staff turnover is a prominent HRM problem in humanitarian organisations. In the profit sector, HRM tools such as pay, benefits, socialisation and training have proven to be effective in increasing organisational commitment and decreasing staff turnover. This study explores whether such tool

  12. Luhmann og Organisation

    DEFF Research Database (Denmark)

    Tække, Jesper; Paulsen, Michael

    2008-01-01

      Synopsis - Vi vil i denne artikel udlægge Luhmanns organisationsteori i et fugleperspektiv. Ud fra en videnskabsteoretisk og metametodologisk vinkel henvender vi os især til interesserede i organisationsteori og organisationskommunikation, som gerne vil tilegne sig Luhmanns organisationsteori i...... forhold til hans overordnede teoriramme. Formålet med artiklen er at fremstille, hvorledes man i det hele taget med Luhmanns teoriapparat kan iagttage organisatoriske fænomener. Vi vil indfri dette formål ved at formidle en række grundbegreber i en sammenhæng, der begynder med iagttagelsesbegrebet og...... bevæger sig videre til systembegrebet, forskellige systemtyper, kommunikationsbegrebet og til sidst organisations- og beslutningsbegrebet. Alt i alt er det artiklens ærinde at blotlægge de begrebslige præmisser for Luhmanns organisationsteori....

  13. Performance-oriented Organisation Modelling

    NARCIS (Netherlands)

    Popova, V.; Sharpanskykh, A.

    2006-01-01

    Each organisation exists or is created for the achievement of one or more goals. To ensure continued success, the organisation should monitor its performance with respect to the formulated goals. In practice the performance of an organization is often evaluated by estimating its performance indicato

  14. Boundary organisations and their strategies

    NARCIS (Netherlands)

    Enst, van Wynanda I.; Runhaar, Hens A.C.; Driessen, P.P.J.

    2016-01-01

    This article discusses three empirical cases of boundary organisations, within the context of the Wadden Sea: the Wadden Academy, IMSA Amsterdam and the NCEA. The research aims to provide further insights into how boundary organisations work in practice. The research shows that the role of a boun

  15. Organisational innovations in the Netherlands

    NARCIS (Netherlands)

    Hartog, F. den; Verbruggen, V.

    2000-01-01

    The Netherlands have shown some major technological and economical changes in the field of new production forms and organisation and labour-related innovations. As in other European countries, some autonomous developments like economic developments partly determine the work organisation and the type

  16. Organisational communication and supportive employees

    NARCIS (Netherlands)

    J.A. de Ridder

    2004-01-01

    The importance of the social dimension of organisations is currently a strong focus of emphasis in the literature. From a managerial perspective, however, it is important that the community spirit within an organisation falls in line with its strategic direction. The study discussed in this article

  17. Organising purchasing and (strategic) sourcing

    DEFF Research Database (Denmark)

    Lidegaard, Nina; Boer, Harry; Munkgaard Møller, Morten

    2015-01-01

    or hybrid overall structure, deliver the expected results. Contingency theory predicts that the success of a firm depends on the fit among characteristics of, amongst others, the firm’s processes and organisational structure. The objective of this paper is to propose and illustrate a processbased...... typological theory of purchasing and (strategic) sourcing organisation....

  18. Innovating the Product Development Organisation

    DEFF Research Database (Denmark)

    Andreasen, Mogens Myrup; Hein, Lars

    1997-01-01

    The organisational innovation of the product development function is a doubtful affair since we can hardly describe why a specific organisation works. In this article two comprehensive innovation campaigns in Danish industry are described with reference to the nature, content and results...... of innovative processes and as the crux an attempt to explain principal elements in the changes of attitudes and behaviour in companies....

  19. Organising, Educating... Changing the World

    Science.gov (United States)

    Grayson, John

    2005-01-01

    Over the past few years a constellation of social movements and organisations concerned with issues of globalisation and world poverty have exploded onto the world stage. They have mobilised demonstrations, organised mass gatherings and conferences, created e-networks and websites and become major players in international political lobbying and…

  20. Diffusing Polymers in Confined Microdomains and Estimation of Chromosomal Territory Sizes from Chromosome Capture Data

    Science.gov (United States)

    Amitai, A.; Holcman, D.

    2013-06-01

    Is it possible to extract the size and structure of chromosomal territories (confined domain) from the encounter frequencies of chromosomal loci? To answer this question, we estimate the mean time for two monomers located on the same polymer to encounter, which we call the mean first encounter time in a confined microdomain (MFETC). We approximate the confined domain geometry by a harmonic potential well and obtain an asymptotic expression that agrees with Brownian simulations for the MFETC as a function of the polymer length, the radius of the confined domain, and the activation distance radius ɛ at which the two searching monomers meet. We illustrate the present approach using chromosome capture data for the encounter rate distribution of two loci depending on their distances along the DNA. We estimate the domain size that restricts the motion of one of these loci for chromosome II in yeast.

  1. The Impact of Organisational Learning on Organisational Performance

    Directory of Open Access Journals (Sweden)

    Anna Zgrzywa-Ziemak

    2015-12-01

    Full Text Available Purpose: The aim of this article is to analyse the theoretical views and results of empirical research concerning the relation between organisational learning (OL and organisational performance (OP. Methodology: The study was carried out through extensive literature research, including relevant literature review from databases such as ProQuest, Elsevier, Emerald and EBSCO (the phrases: “organisational learning”, “learning organisation” and “organisational performance” were searched in the keywords, titles or abstracts. Findings: From a theoretical point of view, the relation between OL and OP is neither obvious nor clear, but the analysis of the empirical studies allows one to assume that OL has an essential impact on OP. However, differences in the strength of the relation were shown and some contradictions related to the presence of the relation between OL and selected (mostly financial performance aspects identified. Furthermore, the article discusses the significant differences and inconsistencies in the methods of measuring OL, measuring OP, selecting contextual factors and adopted methods of data analysis. Implications: Inconsistencies and gaps found in the studies of the relationship between OL and OP made it possible to designate the direction for promising further research. Value: The article presents valuable insight through its in-depth, critical analysis of the organisational learning and organisational outcomes. First and foremost, this indicates that the formula of the previous empirical studies does not allow for the development of precise solutions pertaining to organisational learning management for the benefit of OP improvement.

  2. Intergovernmental organisation activities

    International Nuclear Information System (INIS)

    This section treats of the following Intergovernmental organisation activities: 1 - European Atomic Energy Community: Non-legally binding instruments - Communication from the European Commission 'Towards an Integrated Strategic Energy Technology (SET) Plan: Accelerating the European Energy System Transformation'; 2014 Annual Report of the Euratom Supply Agency; Report of June 2015 from the Euratom Supply Agency to the European Commission on the Security of Supply of Medical Radioisotopes; 2 - International Atomic Energy Agency (IAEA): Convention on Nuclear Safety (CNS); 59. regular session of the IAEA General Conference (Resolutions of the Conference, Measures to Strengthen International Cooperation in Nuclear, Radiation, Transport and Waste Safety (GC(59)/RES/9): conventions, regulatory frameworks and supporting non-legally-binding instruments for safety, Nuclear liability, National infrastructures, Nuclear installation safety, Safe management of radioactive sources, Nuclear and radiological incident and emergency preparedness and response); Nuclear Security (GC(59)/RES/10); IAEA Treaty Event; Legislative assistance activities; Nuclear Law Institute; 3 - OECD Nuclear Energy Agency (NEA): European Nuclear Energy Tribunal (ENET) Inaugural Session for the 9. mandate; New signatories to the extension of the Generation IV International Forum (GIF) Framework Agreement; Joint Declaration on the Security of Supply of Medical Radioisotopes; International Framework for Nuclear Energy Cooperation (IFNEC); 15. session of the International School of Nuclear Law (ISNL); 2016 session of the International Nuclear Law Essentials (INLE)

  3. Chromosome landmarks and autosome-sex chromosome translocations in Rumex hastatulus, a plant with XX/XY1Y2 sex chromosome system.

    Science.gov (United States)

    Grabowska-Joachimiak, Aleksandra; Kula, Adam; Książczyk, Tomasz; Chojnicka, Joanna; Sliwinska, Elwira; Joachimiak, Andrzej J

    2015-06-01

    Rumex hastatulus is the North American endemic dioecious plant with heteromorphic sex chromosomes. It is differentiated into two chromosomal races: Texas (T) race characterised by a simple XX/XY sex chromosome system and North Carolina (NC) race with a polymorphic XX/XY1Y2 sex chromosome system. The gross karyotype morphology in NC race resembles the derived type, but chromosomal changes that occurred during its evolution are poorly understood. Our C-banding/DAPI and fluorescence in situ hybridization (FISH) experiments demonstrated that Y chromosomes of both races are enriched in DAPI-positive sequences and that the emergence of polymorphic sex chromosome system was accompanied by the break of ancestral Y chromosome and switch in the localization of 5S rDNA, from autosomes to sex chromosomes (X and Y2). Two contrasting domains were detected within North Carolina Y chromosomes: the older, highly heterochromatinised, inherited from the original Y chromosome and the younger, euchromatic, representing translocated autosomal material. The flow-cytometric DNA estimation showed ∼3.5 % genome downsizing in the North Carolina race. Our results are in contradiction to earlier reports on the lack of heterochromatin within Y chromosomes of this species and enable unambiguous identification of autosomes involved in the autosome-heterosome translocation, providing useful chromosome landmarks for further studies on the karyotype and sex chromosome differentiation in this species.

  4. Using organisational memory in evaluations

    Directory of Open Access Journals (Sweden)

    Madri S. Jansen van Rensburg

    2014-12-01

    Full Text Available This article uses the case of a regional intermediary organisation to investigate organisational memory (OM and its contribution to knowledge management and activities in evaluations. Understanding of, and accessing OM is critical for participatory evaluations. The aim of the article is to reflect on the OM of a non-governmental organisation (NGO and what implicationsthe structural changes in OM over the organisation’s life cycle have for evaluators. It further aims to advocate an awareness of OM and explains how evaluators can access and utilise it more effectively. Evaluators need to have an understanding of OM, and to take more responsibility for disseminating results to enhance it. This case study reflects on a retrospective case example of a regional NGO. The report reflects the development and structure of the life cycle of the organisation. The data collection included in-depth interviews with staff members and other key stakeholders, engagement with beneficiary organisations and donors, and analyses of documents, electronic files and audio-visual material. Since OM survives after the demise of an organisation, and is accessible through directories, it is important for the evaluator to include historical information. Specific implications for evaluators include the ability to access OM through directories and networks of the organisation. As evaluators hold OM of all the organisations they have engaged with, they also have a responsibility to share knowledge. The key findings of this study illustrate the importance of accessing the memory and historical information of the organisation. Understanding OM enhances the in-depth comprehension of the activity, project or programme under investigation, and the collective knowledge generated as a result of it.

  5. The construct validation of an instrument designed to assess organisational socialisation

    Directory of Open Access Journals (Sweden)

    Michelle Mudurai

    2008-09-01

    Full Text Available The aim of this study was to establish the construct validity of an instrument for assessing organisational socialisation. A purposive sample of 170 respondents completed the socialisation instrument. Scale reliabilities and a confrmatory factor analysis confrmed six constructs (History, Language, Politics, People, Organisational Goals and Values, and Performance Profciency as measures of this domain. The signifcance of the fndings of this study is that from a South African perspective, the socialisation instrument can be useful in measuring organisational socialisation. This instrument can also be valuable in assessing the effectiveness of the socialisation tactics used by organisations.

  6. Identity In and Around Organisations

    DEFF Research Database (Denmark)

    Schultz, Majken; Maguire, Steve

    2013-01-01

    concept may not be the best way of approaching and managing your organisation. Rather, Majken Schultz and Steve Maguire argue that organisations would benefit from adopting a process-based view of identity, which integrates history, ongoing change and market instability into its definition.......At the heart of any successful organisation lies a powerful conception of identity: the coherent way in which it presents itself to its stakeholders and employees, containing its purpose, goals and key characteristics. However, the traditional idea of identity as a stable, solid and reliable...

  7. Building a strategic security organisation.

    Science.gov (United States)

    Howard, Mike

    2016-01-01

    In everyone's day-to-day jobs there is constant need to deal with current and newly detected matters. This is now a world of immediacy, driven by the cadence of the business and its needs. These concerns should not be ignored, as failing to deal with these issues would not bode well for the future. It is essential that the gears are kept spinning. The challenge for any security organisation is to identify its short-term tactical requirements, while developing longer-term strategic needs. Once done, the differences can be accounted for and strides can be made toward a desired future state. This paper highlights several steps that the author and his team have taken in their own journey. There is no magic answer, each organisation will have its own unique challenges. Nevertheless, some of the approaches to building a strategic security organisation described in this paper are applicable to all organisations, irrespective of their size. PMID:27318284

  8. The Strategic Process in Organisations

    DEFF Research Database (Denmark)

    Sørensen, Lene; Vidal, Rene Victor Valqui

    1999-01-01

    Organisational strategy development is often conceptualised through methodological frameworks. In this paper strategy development is seen as a strategic process characterised by inherent contradictions between actors, OR methods and the problem situation. The paper presents the dimensions...

  9. Cultural Diversity in Multinational Organisations

    OpenAIRE

    Crowley-Henry, Marian

    2005-01-01

    With the rhetoric in international management espousing the value of being able to access and capitalise on the knowledge of a workforce with international experience in order to compete globally and the need to embrace diversity (including cultural or ethnic diversity) in and across organisations, this paper discusses the findings from a qualitative research undertaking where senior and middle managers working for multinational organisations in a cross-section of industry sectors were ...

  10. Chinese Pragmatism and the Learning Organisation

    Science.gov (United States)

    Elkin, Graham; Cone, Malcolm H.; Liao, Jianqiao

    2009-01-01

    Purpose: For 40 years, it has been widely believed in the West that learning organisations would be healthier, flexible and more competitive than other organisations. By now, one might expect them to be widespread. However, fully developed learning organisations are rare in the West. In contrast, Chinese organisations seem naturally to be learning…

  11. From Digital Administration to Organisational Learning

    Science.gov (United States)

    Elkjaer, Bente

    2005-01-01

    Purpose: To explore whether deliberate organisational change of a public sector organisation (a local municipality) would create an avenue for organisational learning. Design/methodology/approach: A case study was set up to study the means by which the organisational change towards a digital administration was to come about. The organisational…

  12. Condensin-Based Chromosome Organization from Bacteria to Vertebrates.

    Science.gov (United States)

    Hirano, Tatsuya

    2016-02-25

    Condensins are large protein complexes that play a central role in chromosome organization and segregation in the three domains of life. They display highly characteristic, rod-shaped structures with SMC (structural maintenance of chromosomes) ATPases as their core subunits and organize large-scale chromosome structure through active mechanisms. Most eukaryotic species have two distinct condensin complexes whose balanced usage is adapted flexibly to different organisms and cell types. Studies of bacterial condensins provide deep insights into the fundamental mechanisms of chromosome segregation. This Review surveys both conserved features and rich variations of condensin-based chromosome organization and discusses their evolutionary implications.

  13. Domains and domain loss

    DEFF Research Database (Denmark)

    Haberland, Hartmut

    2005-01-01

    The domain concept, originally suggested by Schmidt-Rohr in the 1930’s (as credited in Fishman’s writings in the 1970s), was an attempt to sort out different areas of language use in multilingual societies, which are relevant for language choice. In Fishman’s version, domains were considered...... not described in terms of domains, and recent research e.g. about the multilingual communities in the Danish-German border area seems to confirm this....

  14. The effect of organisational context on organisational development (OD interventions

    Directory of Open Access Journals (Sweden)

    Sanjana Brijball Parumasur

    2012-01-01

    Full Text Available Orientation: Systematic and congruent organisational structures, systems, strategies and designs are necessary for the successful implementation of organisational development (OD interventions.Research purpose: This article examines national and international OD practices. It assesses the effect of diverse cultures and cultural values for determining the effectiveness of OD interventions.Motivation for the study: Most organisational change and development programmes fail and only a few result in increased competitiveness, improvements and profitability. This emphasises the need for change interventions to give sufficient attention to leadership, cultures, managing change and adopting context-based OD interventions.Research design, approach and method: This article is a literature review of the current trends and research in the area of OD interventions. It synthesises the influence that cultures and cultural orientations have on determining which OD intervention strategies organisations should adopt in different cultures.Main findings: The analysis emphasises how important it is to achieve congruence between the OD interventions organisations select and their local cultures.Practical/managerial implications: It is important to note the evolving nature of the political and economic climates that influence national cultures and that they emphasise that interventions that reflect OD values, which are tailor-made and shaped to the needs of local cultures, are necessary.Contribution/value-add: This study links various OD interventions to Hofstede’s dimensions for differentiating national cultures. It provides guidelines for aligning the practices and techniques of OD to the values and cultures of the organisations and societies in which they are to be implemented.

  15. Constructing professional and organisational fields.

    Science.gov (United States)

    Gurney, Robert

    2016-01-01

    Purpose - The purpose of this paper is to fill an apparent gap in the literature addressing issues of leadership and change - the development and activities of constructing and leading sports sciences and medicine professions, and similarly, the construction and leadership of multidisciplinary/inter-disciplinary organisations that practice sports sciences and medicine. Design/methodology/approach - This study incorporated explorations through conducting both interviews and survey questionnaires with members of Sports Medicine Australia (SMA). The interviews (qualitative) were semi-structured and asked questions addressing what changed, why change and how change was implemented. Findings - The health sciences and medicine professions moving to specialised sports sciences and medicine disciplines and SMA, evolved through forces driving the need for change (legitimacy, resource dependency, positioning and core competencies). Practical implications - The knowledge developed from understanding activities of change that traditional professions conducted to become specialised Disciplines and parallel changes in a single Discipline organisation evolving to an umbrella organisation (SMA), comprised a membership of specialised Disciplines, can act as a catalyst for inquiry by other professional and organisational groups. Originality/value - The findings of this study contributes to the literature investigating change in professional and organisations fields. More specifically, this study promotes inquiry into leadership practices of sports sciences and medicine, as contributors to the field of health services. PMID:26764964

  16. Virtues for innovativeness : a mixed methods study of ethical organisational culture and organisational innovativeness

    OpenAIRE

    Riivari, Elina

    2016-01-01

    This study examined ethical organisational culture, consisting of ethical organisational virtues, and organisational innovativeness. More specifically, the study explored the relationship between the phenomena. Ethical organisational culture was studied using Corporate Ethical Virtues (CEV) model, which is based on the virtue theory of business ethics. This research makes a contribution to prior research on ethical organisational culture by linking the concept of organ...

  17. Organisational Diversity and Knowledge Sharing

    DEFF Research Database (Denmark)

    Lauring, Jakob; Jonasson, Charlotte

    The last years' focus on diversity management has gone from social responsibility to arguments for the competitive advantages, called the business case. It has been argued that diversity management can increase organisational efficiency, improve on moral, and give better access to new market...... segments. But a substantial critique has recently been launched at the arguments for the business case for diversity, especially in the employment of knowledge synergies. Most studies that support or reject the business case argument have limited their research to establish a link between organisational...... productivity and diversity management - that is not engaging in a discussion of how and why there could be potential benefits or difficulties. In this paper we will describe and analyse some of the complications multicultural organisations can experience in regard to knowledge sharing...

  18. EEN encodes for a member of a new family of proteins containing an Src homology 3 domain and is the third gene located on chromosome 19p13 that fuses to MLL in human leukemia

    OpenAIRE

    So, Chi Wai; Caldas, Carlos; Liu, Meng-Min; Chen, Sai-Juan; Huang, Qiu-Hua; Gu, Long-Jun; Sham, Mai Har; Wiedemann, Leanne Marie; Chan, Li Chong

    1997-01-01

    The MLL gene, the closest human homologue to the Drosophila trithorax gene, undergoes chromosomal translocation with a large number of different partner genes in both acute lymphoid and acute myeloid leukemias. We have identified a new partner gene, EEN, fused to MLL in a case of acute myeloid leukemia. The gene is located on chromosome 19p13, where two other MLL partner genes, ENL and ELL/MEN have also been identified. The deduced protein of 368 aa contains a central α-helical region and a C...

  19. Environmental health organisations against tobacco.

    LENUS (Irish Health Repository)

    Mulcahy, Maurice

    2009-04-01

    Implementing the World Health Organisation (WHO) Framework Convention on Tobacco Control (FCTC) relies heavily on enforcement. Little is known of the way different enforcement agencies operate, prioritise or network. A questionnaire was sent to representatives of the International Federation of Environmental Health (IFEH) in 36 countries. Tobacco control was given low priority. Almost two thirds did not have any tobacco control policy. A third reported their organisation had worked with other agencies on tobacco control. Obstacles to addressing tobacco control included a lack of resources (61%) and absence of a coherent strategy (39%).

  20. Seeing Like an International Organisation

    DEFF Research Database (Denmark)

    Broome, André; Seabrooke, Leonard

    2012-01-01

    International organisations (IOs) often serve as the ‘engine room’ of ideas for structural reforms at the national level, but how do IOs construct cognitive authority over the forms, processes and prescriptions for institutional change in their member states? Exploring the analytic institutions...... and organisational environment that guides an IO's actions and informs its policy advice to states, which enables a more comprehensive picture of how the everyday business of global governance works in practice. Instead of ‘black boxing’ IOs, the contributors to this special issue demonstrate how studying IOs from...

  1. Cultural Diversity and Organisational Effiency

    DEFF Research Database (Denmark)

    Lauring, Jakob

    2004-01-01

    The expected positive link between diversity management and organisational efficiency is often used as a reason for pursuing diversity management and equal employment opportunity programmes. However, this positive link is only supported to a limited degree by in-depth empirical research and there......The expected positive link between diversity management and organisational efficiency is often used as a reason for pursuing diversity management and equal employment opportunity programmes. However, this positive link is only supported to a limited degree by in-depth empirical research...

  2. PREFACE: Scientific Organising Committee Information

    Science.gov (United States)

    2015-06-01

    Edited by: Oliver Roberts Lorraine Hanlon Sheila McBreen Local Organising Committee: Oliver Roberts (Chair) Antonio Martin-Carrillo Lorraine Hanlon Sheila McBreen Alexey Uliyanov David Murphy Sinéad Hales Scientific Organising Committee:: Sheila McBreen, (UCD, Ireland) (Chair) Franco Camera (INFN-Milano, Italy) Nerine Cherepy (LLNL, USA) Jarek Glodo (RMD, USA) Lorraine Hanlon (UCD, Ireland) Paul Lecoq (CERN, Switzerland) Julie McEnery (NASA, USA) Oliver Roberts (UCD, Ireland) Anant Setlur, (GE, USA) Brian Shortt, (ESA, the Netherlands) Kenneth Stanton (UCD, Ireland)

  3. Organisational Conditions for Corporate Entrepreneurship in Dutch Organisations

    NARCIS (Netherlands)

    Veenker, Simon; Sijde, van der Peter; During, Wim; Nijhof, André

    2008-01-01

    Corporate entrepreneurship is a topic of attraction for many managers in corporate enterprises. In the early 1980s, several researchers discovered the importance of entrepreneurship and its role in organisational renewal, innovation, risk taking and creation of new businesses. Corporate entrepreneur

  4. Dimensions and Domains of Organisational Effectiveness in Australian Higher Education.

    Science.gov (United States)

    Lysons, Art

    1990-01-01

    Research in Australian higher education testing one theory of dimensions of organizational effectiveness is reviewed, and two theories are analyzed to develop a more comprehensive set of dimensions. The existing taxonomy is further defined, and the implications relating to recent structural adjustments in the higher education system are discussed.…

  5. An Exploration of Policy Making in Public and Organisational Domains

    Institute of Scientific and Technical Information of China (English)

    谢苑苑

    2014-01-01

    This article critically analyses the interplay between policy at institutional level and policy at national level. It also examines the policy-making process by using examples from literature and identifies consultation as a more organic way of policy-making.

  6. Stupid Organisation - How will you ever learn?

    DEFF Research Database (Denmark)

    Elkjær, Bente

    2005-01-01

     The question derives from a research project in which I explored whether a deliberate change process in an organisation would lead to organisational learning. The idea was to see whether it was possible to depict the unfolding of organisational learning processes in the turmoil of change...... in an organisation. If this is the case we may be able to provide clues as to how to deliberately support organisational processes of learning. The study was a case study of a local municipality in Denmark that was pursuing a change from a conventional to a digitalised organisation. The theoretical approach...... was to see whether a pragmatic understanding of learning and organising could open up the organisational learning process. The work took me to a conclusion in which I point to the value of tensions in organisations. It also made me stress the necessity of an awareness of tensions being bridgeable...

  7. Undetected sex chromosome aneuploidy by chromosomal microarray.

    Science.gov (United States)

    Markus-Bustani, Keren; Yaron, Yuval; Goldstein, Myriam; Orr-Urtreger, Avi; Ben-Shachar, Shay

    2012-11-01

    We report on a case of a female fetus found to be mosaic for Turner syndrome (45,X) and trisomy X (47,XXX). Chromosomal microarray analysis (CMA) failed to detect the aneuploidy because of a normal average dosage of the X chromosome. This case represents an unusual instance in which CMA may not detect chromosomal aberrations. Such a possibility should be taken into consideration in similar cases where CMA is used in a clinical setting.

  8. Organisational change. Grace under fire.

    Science.gov (United States)

    Dickinson, Helen; Parker, Helen

    2006-12-14

    The success of organisational change is often thwarted by leaders' failure to consider staff feelings. Managers must communicate a clear vision for the future, even though they may be facing great professional uncertainty themselves. It is important to deal with post-merger issues such as helping staff to new roles and 'unlearning' old ways. PMID:17252855

  9. 'Ethos' Enabling Organisational Knowledge Creation

    Science.gov (United States)

    Matsudaira, Yoshito

    This paper examines knowledge creation in relation to improvements on the production line in the manufacturing department of Nissan Motor Company and aims to clarify embodied knowledge observed in the actions of organisational members who enable knowledge creation will be clarified. For that purpose, this study adopts an approach that adds a first, second, and third-person's viewpoint to the theory of knowledge creation. Embodied knowledge, observed in the actions of organisational members who enable knowledge creation, is the continued practice of 'ethos' (in Greek) founded in Nissan Production Way as an ethical basis. Ethos is knowledge (intangible) assets for knowledge creating companies. Substantiated analysis classifies ethos into three categories: the individual, team and organisation. This indicates the precise actions of the organisational members in each category during the knowledge creation process. This research will be successful in its role of showing the indispensability of ethos - the new concept of knowledge assets, which enables knowledge creation -for future knowledge-based management in the knowledge society.

  10. Entrepreneuring as Organisation-Creation

    DEFF Research Database (Denmark)

    Hjorth, Daniel

    2014-01-01

    This chapter aims at making a contribution to the study of entrepreneurship and creativity by developing a processual conceptualisation of a form of entrepreneurial creativity called entrepreneuring or organisation-creation. Such a processual conceptualisation of entrepreneuring will answer the l...

  11. The Organisation as Artist's Palette

    DEFF Research Database (Denmark)

    Schnugg, Claudia

    2014-01-01

    The article considers the many possibilities of bringing the arts into organisations to keep up with the demands of an uncertain and fast-changing environment. It discusses cases of arts-based interventions in companies that reflect the different kinds of arts-based interventions that can be found...

  12. The Immigrant Organising Process : Turkish Organisations in Amsterdam and Berlin and Surinamese Organisations in Amsterdam, 1960-2000

    OpenAIRE

    Vermeulen, Floris

    2006-01-01

    This study focuses on the emergence and persistence of immigrant organisations in host societies. The relevance of immigrant organisations for both the host society and the immigrants themselves has been effectively demonstrated in many different studies. However, the question why immigrant organisations emerge and why they often persist over a long period is not adequately answered. In this study a comparative approach is used to reveal the structural determinants of the immigrant organising...

  13. Importance of the hexagonal lipid phase in biological membrane organisation

    Directory of Open Access Journals (Sweden)

    Juliette eJouhet

    2013-12-01

    Full Text Available Abstract:Domains are present in every natural membrane. They are characterised by a distinctive protein and/or lipid composition. Their size is highly variable from the nano- to the micrometer scale. The domains confer specific properties to the membrane leading to original structure and function. The determinants leading to domain organisation are therefore important but remain obscure. This review presents how the ability of lipids to organize into hexagonal II or lamellar phases can promote particular local structures within membranes. Since biological membranes are composed of a mixture of lipids, each with distinctive biophysical properties, lateral and transversal sorting of lipids can promote creation of domains inside the membrane through local modulation of the lipid phase. Lipid biophysical properties have been characterized for long based on in vitro analyses using non-natural lipid molecules; their re-examinations using natural lipids might open interesting perspectives on membrane architecture occurring in vivo in various cellular and physiological contexts.

  14. Fetal chromosome analysis: screening for chromosome disease?

    DEFF Research Database (Denmark)

    Philip, J; Tabor, Ann; Bang, J;

    1983-01-01

    The aim of the study was to investigate the rationale of the current indications for fetal chromosome analysis. 5372 women had 5423 amniocentesis performed, this group constituting a consecutive sample at the chromosome laboratory, Rigshospitalet, Copenhagen from March 1973 to September 1980 (Group...... A + B). Pregnant women 35 years of age, women who previously had a chromosomally abnormal child, families with translocation carriers or other heritable chromosomal disease, families where the father was 50 years or more and women in families with a history of Down's syndrome (group A), were compared...... to women having amniocentesis, although considered not to have any increased risk of fetal chromosome abnormality (1390 pregnancies, group B). They were also compared with 750 consecutive pregnancies in women 25-34 years of age, in whom all heritable diseases were excluded (group C). The risk of unbalanced...

  15. Chromosome Disorder Outreach

    Science.gov (United States)

    ... BLOG Join Us Donate You are not alone. Chromosome Disorder Outreach, Inc. is a non-profit organization, ... Support For all those diagnosed with any rare chromosome disorder. Since 1992, CDO has supported the parents ...

  16. A Case Study of Horizontal Reuse in a Project-Driven Organisation

    DEFF Research Database (Denmark)

    Christensen, Henrik Bærbak; Røn, Henrik

    2000-01-01

    This experience paper presents observations, lessons learned, and recommendations based on a case study of reuse. The case study is concerned with the development, maturation, and reuse of a business domain independent software component (horizontal reuse) in a project-driven organisation that has...... knowledge is transferred within an organisation; (c) design patterns can be as risky as they can be beneficial; and (d) there is more to architectural mismatch than “merely ” packaging mismatch....

  17. ORGANISATIONAL SOCIAL MEDIA PLATFORMS: EXPLORING USER PARTICIPATION BEHAVIOURS IN SOFTWARE AND TECHNOLOGY FIRMS

    OpenAIRE

    Demetriou, Georgia

    2012-01-01

    The aim of this research was to explore the user participation behaviours in the emerging structure of organisational social media platforms; a term coined and defined in this thesis. This emerging community structure originates from technical discussion forums and knowledge repository systems, and appears to be concerned with solving user problems, generating professional and technical content, and facilitating interaction in the external organisational domain. This research has explored thr...

  18. Bullying and Organisational Commitment: Common antecedents?

    OpenAIRE

    Dick, Gavin P.M.

    2011-01-01

    The paper’s aim is to provide new theoretical insights by examining whether organisational commitment and workplace bullying co-vary, and if this is due to direct effects and/or indirect effects of their organisation and supervision environment. From a survey of all uniform officers in a UK police agency the author analyses the bullying behaviours experienced by police officers and if the organisational and managerial factors that are known to influence organisational commitment also change t...

  19. EMPLOYEE LEARNING AND DEVELOPMENT IN ORGANISATIONS

    OpenAIRE

    VNOUČKOVÁ, Lucie

    2013-01-01

    The primary goal of all organisations is efficiency of human resources. Therefore activities as HR controlling, performance management but also cutting costs are the main theme. Current organisations need to monitor human resources to keep their competitiveness. Thus paper describes the key factor of organisational efficiency - employee education, talent management and the necessity to retain skilled employees. The aim of the paper is to reveal the current approach in organisations to educati...

  20. Effective Diagnosis in Organisation Change Management

    OpenAIRE

    Ahmed Hassin

    2014-01-01

    Organisations are part of interactive and dynamic environments and modern organisations face considerable pressure to meet or exceed customer/ beneficiary’s and stakeholders’ expectations. The key to establishing effective change and transformation in organisations lies in the early stages of assessment and diagnosis: if diagnosis is wrong, treatment will be ineffective. An organisation development programme should be based on a sound analysis of relevant data about the problem situation, and...

  1. Organisational learning as movements in practice

    DEFF Research Database (Denmark)

    Elkjær, Bente

    2013-01-01

    In the paper, I take the readers through a tour de force of the past, present and future of the field of organisational learning. This is structured around three concepts that stand out as important, namely organisational learning as changed behaviour, as changed theories of actions and as part...... of practice. I also point to the future of organisational learning as inspired by the work of pragmatist philosophy and as affected by the call for more concreteness in organisation studies as a whole....

  2. Refugee community organisations: a social capital analysis

    OpenAIRE

    Kellow, Alexa

    2011-01-01

    This thesis considers how refugee-led community organisations generate social capital for their service users. The concept of social capital has become popular in policy debates in recent years, and previous research has attributed social capital creation for their service users to refugee community organisations (RCOs). This research aimed to analyse the process by which social capital is created by refugee community organisations, and what this means for the members of these organisations i...

  3. ZEBRAFISH CHROMOSOME-BANDING

    NARCIS (Netherlands)

    PIJNACKER, LP; FERWERDA, MA

    1995-01-01

    Banding techniques were carried out on metaphase chromosomes of zebrafish (Danio rerio) embryos. The karyotypes with the longest chromosomes consist of 12 metacentrics, 26 submetacentrics, and 12 subtelocentrics (2n = 50). All centromeres are C-band positive. Eight chromosomes have a pericentric C-b

  4. Chromosome painting in plants.

    NARCIS (Netherlands)

    Schubert, I.; Fransz, P.F.; Fuchs, J.; Jong, de J.H.

    2001-01-01

    The current 'state-of-art' as to chromosome painting in plants is reviewed. We define different situations described as painting so far: i) Genomic in situ hybridisation (GISH) with total genomic DNA to distinguish alien chromosomes on the basis of divergent dispersed repeats, ii) 'Chromosomal in si

  5. Domains and domain loss

    DEFF Research Database (Denmark)

    Haberland, Hartmut

    2005-01-01

    politicians and in the media, especially in the discussion whether some languages undergo ‘domain loss’ vis-à-vis powerful international languages like English. An objection that has been raised here is that domains, as originally conceived, are parameters of language choice and not properties of languages...... theoretical constructs that can explain language choice which were supposed to be a more powerful explanatory tool than more obvious (and observable) parameters like topic, place (setting) and interlocutor. In the meantime, at least in Scandinavia, the term ‘domain’ has been taken up in the debate among...

  6. Improving Teaching Quality and the Learning Organisation

    Science.gov (United States)

    Collie, Sarah L.; Taylor, Alton L.

    2004-01-01

    This study applied a learning organisation framework to understand academic departments' efforts to improve teaching quality. The theoretical framework was generated from literature on learning organisations, organisations devoted to continuous improvement through continuous learning. Research questions addressed relationships among departments'…

  7. Key variables of organisation design in servitization

    DEFF Research Database (Denmark)

    Kreye, Melanie; Jensen, Per Langaa

    2014-01-01

    Manufacturing companies offering additional service with their products need to change their organisation design to accommodate for the service business. Using the star model, this paper investigates organisation design in terms of strategy, structure, processes, rewards and people as a response......, and (iii) the parts of organisation design need to be aligned to prevent inconsistencies in service provision....

  8. Reflection as a Catalyst for Organisational Learning

    Science.gov (United States)

    Knipfer, Kristin; Kump, Barbara; Wessel, Daniel; Cress, Ulrike

    2013-01-01

    This article takes a psychological perspective on organisational learning, putting "reflection" into the centre of attention. We argue that (1) organisational learning is based on individual and team learning at work, (2) reflection is the driving force that leads to organisational learning and (3) cumulation of the staff's reflection outcomes…

  9. Cryogenic safety organisation at CERN

    CERN Document Server

    CERN. Geneva

    2016-01-01

    With Safety being a top priority of CERN’s general policy, the Organisation defines and implements a Policy that sets out the general principles governing Safety at CERN. To the end of the attainment of said Safety objectives, the organic units (owners/users of the equipment) are assigned the responsibility for the implementation of the CERN Safety Policy at all levels of the organization, whereas the Health and Safety and Environmental Protection Unit (HSE) has the role of providing assistance for the implementation of the Safety Policy, and a monitoring role related to the implementation of continuous improvement of Safety, compliance with the Safety Rules and the handling of emergency situations. This talk will elaborate on the roles, responsibilities and organisational structure of the different stakeholders within the Organization with regards to Safety, and in particular to cryogenic safety. The roles of actors of particular importance such as the Cryogenic Safety Officers (CSOs) and the Cryogenic Sa...

  10. Organising pneumonia due to dronedarone.

    Science.gov (United States)

    Thornton, D; Avery, S; Edey, A J; Medford, A R L

    2015-01-01

    Organising pneumonia is one of the responses of the lung to injury and can mimic bacterial pneumonia but importantly it does not respond to antibiotic therapy. We present the case of a 67-year-old male who was diagnosed with organising pneumonia secondary to dronedarone. Drug reactions are a common cause and early identification of the culprit is mandatory to prevent further morbidity and ensure a favourable outcome. On chest radiography there may be fleeting peripheral consolidation, while computed tomography can show a range of stereotyped patterns including perilobular consolidation. Bronchoscopic biopsy may not always be possible but response to steroids is often rapid following removal of the culprit drug. Dronedarone should be included in the list of possible drugs and the Pneumotox database remains a useful resource for the clinician when acute drug-related pneumotoxicity is suspected. PMID:26517101

  11. Spreadsheet Risk Management in Organisations

    CERN Document Server

    Rittweger, Ben G

    2010-01-01

    The paper examines in the context of financial reporting, the controls that organisations have in place to manage spreadsheet risk and errors. There has been widespread research conducted in this area, both in Ireland and internationally. This paper describes a study involving 19 participants (2 case studies and 17 by survey) from Ireland. Three areas are examined; firstly, the extent of spreadsheet usage, secondly, the level of complexity employed in spreadsheets, and finally, the controls in place regarding spreadsheets. The findings support previous findings of Panko (1998), that errors occur frequently in spreadsheets and that there is little or unenforced controls employed, however this research finds that attitudes are changing with regard to spreadsheet risk and that one organisation is implementing a comprehensive project regarding policies on the development and control of spreadsheets. Further research could be undertaken in the future to examine the development of a "best practice model" both for t...

  12. In Pursuit of Organisational Renewal

    DEFF Research Database (Denmark)

    Sørensen, Brian Vejrum

    Every time we open a newspaper we are confirmed in the belief that organisations are faced by increas-ingly knowledge intensive realities. More often than not, these reports tell us how companies are strug-gling with issues related to operating on an competitive stage, which is characterised...... is another mat-ter, which is less explored, especially when we look at implementation and sustaining of the initiatives taken. The concern of the thesis, therefore, rests with the constructive process generating new systems, practices and understandings that improve organisational competitiveness. The thesis...... employs three longitudinal case studies, which follow three companies going through deep change, which challenged their capacities to act and learn. This prompted the companies to work with knowledge and learning in several dimensions, related to reflective inquiry, sensing opportunities, and development...

  13. Understanding bullying in healthcare organisations.

    Science.gov (United States)

    Allen, Belinda

    2015-12-01

    Bullying is a pervasive problem in healthcare organisations. Inquiries and reports on patient care and poor practice in the NHS have emphasised the substantial negative effects this behaviour may have on patient care. If bullying is to be addressed, it is crucial we develop clarity about what behaviours constitute bullying and how these behaviours differ from other negative behaviours in the workplace. It is important that we recognise the extent of the problem; statistics on the prevalence of bullying are likely to be an underestimate because of under-reporting of bullying. Effective interventions may only be designed and implemented if there is knowledge about what precipitates bullying and the magnitude of the changes required in organisations to tackle bullying. Individuals should also be aware of the options that are available to them should they be the target of bullying behaviour and what they should do if they witness bullying in their workplace. PMID:26639294

  14. Cemeteries - organisation, management and innovation

    DEFF Research Database (Denmark)

    Kjøller, Christian Philip

    Through the use of qualitative methods and various theoretical perspectives the implementation of maintenance specifications for Danish cemeteries is analysed and in parallel the cemeteries are described and characterised. Cemeteries and their management is complex due to the duality of the servi...... and thus likely to be part of future innovation and learning processes. Study findings contribute to the field of Danish cemeteries, as well as the knowledge base on cemeteries, public green spaces and of organisational change....... and due to frequent ad hoc approaches. The new tool represents a divergent change of current practices, and implementation is subsequently ambiguous, with adaptations of both tool and organisations. The tool is however addressing aspects of emerging challenges in the field of cemetery administrations...

  15. Feminine leadership and organisational culture

    Directory of Open Access Journals (Sweden)

    Andreea-Simona Saseanu

    2014-05-01

    Full Text Available People in general, leaders especially, are influenced by the organisational culture and the other way around. Organisational culture represents a determining factor regarding the display of leadership, since these two processes create each other, adding value and consistency to one another. Moreover, organisational culture can be created and developed in a fluctuant business environment, in which the external factors influence its progress. The development of society has incessantly been emphasized by the relationship between the sexes, by their individual evolution, but also by the interdependency between them. Although there is increasingly more talk about gender equality, in many countries, the social as well as economical chances and opportunities are not equal for women and men. Gradually, women have been through a series of changes related to education, rights and obligations. However, the number of women leaders has always been much lower than the number of men leaders. The personality traits of leaders significantly influence the leadership style and the way in which they are perceived by their subordinates, all this having a major impact on the overall performances of the organisation. In this manner, depending on the gender (masculine/feminine, we can state that one could observe certain personality traits that are characteristic to women and others specific to men, leaving their mark on the leadership method and on the leadership style adopted of each of them. Considering the fact that, in the current turbulent economic environment, certain qualities such as flexibility, intuition, development of communication networks and motivating the employees represent values that are considered to be “feminine”, one can assert that, in this case, gender is an opportunity. However, if we should take into consideration the impact of culture, of certain mentalities and misconceptions that are still present, regarding the woman’s standing in

  16. Communication in third sector organisations

    Directory of Open Access Journals (Sweden)

    Patricia Durán-Bravo, M.A.

    2010-01-01

    Full Text Available Third sector organisations favour the social development due to their capacity to produce human and social capital in society (Putman, 2001. In order to achieve social welfare third sector organisations need to produce a positive change in the relations among individuals, groups and institutions in a society. The expectations about the third sector organisations’ transparency and capacity to tackle social problems are increasing worldwide. In Mexico, this problematic is more complex because there is a low level of professionalism, transparency, accountability and participation culture in the organized civil society (ITAM, 2010. The development of the organized civil society in Mexico will depend to a great extent on its capacity to communicate its values to society and achieve a cultural and social change. Therefore, the third sector organisations must adopt strategic initiatives to reinforce their credibility and reputation, because the public and private donors are increasingly more demanding in their criteria to grant resources to social projects; and because the Mexican society has a low participation level and faces increasingly more complex social problems.

  17. Identification of alternative topological domains in chromatin

    OpenAIRE

    Filippova, Darya; Patro, Rob; Duggal, Geet; Kingsford, Carl

    2014-01-01

    Chromosome conformation capture experiments have led to the discovery of dense, contiguous, megabase-sized topological domains that are similar across cell types and conserved across species. These domains are strongly correlated with a number of chromatin markers and have since been included in a number of analyses. However, functionally-relevant domains may exist at multiple length scales. We introduce a new and efficient algorithm that is able to capture persistent domains across various r...

  18. Multiscale Identification of Topological Domains in Chromatin

    OpenAIRE

    Filippova, Darya; Patro, Rob; Duggal, Geet; Kingsford, Carl

    2013-01-01

    Recent chromosome conformation capture experiments have led to the discovery of dense, contiguous, megabase-sized topological domains that are similar across cell types and conserved across species. These domains are strongly correlated with a number of chromatin markers and have since been included in a number of analyses. However, functionally-relevant domains may exist at multiple length scales. We introduce a new and efficient algorithm that is able to capture persistent domains across va...

  19. Bias of purine stretches in sequenced chromosomes

    DEFF Research Database (Denmark)

    Ussery, David; Soumpasis, Dikeos Mario; Brunak, Søren;

    2002-01-01

    We examined more than 700 DNA sequences (full length chromosomes and plasmids) for stretches of purines (R) or pyrimidines (Y) and alternating YR stretches; such regions will likely adopt structures which are different from the canonical B-form. Since one turn of the DNA helix is roughly 10 bp, we...... measured the fraction of each genome which contains purine (or pyrimidine) tracts of lengths of 10 by or longer (hereafter referred to as 'purine tracts'), as well as stretches of alternating pyrimidines/purine ('pyr/pur tracts') of the same length. Using this criteria, a random sequence would be expected......, in eukaryotes there is an abundance of long stretches of purines or alternating purine/pyrimidine tracts, which cannot be explained in this way; these sequences are likely to play an important role in eukaryotic chromosome organisation....

  20. Organisering

    DEFF Research Database (Denmark)

    Olsen, Poul Bitsch

    2013-01-01

    Organiseringsanalyse er den væsentligste af samtidens analysemetode for ledere og alle der er engagerede i organisationer og erhvervsaktivitet. Her fremlægges organiseringsanalysens baggrund i den amerikanske pragmatisme, og hvorledes den netop udfylder en plads i den mikrosociologiske tradition...

  1. Organisational Learning: Conceptual Links to Individual Learning, Learning Organisation and Knowledge Management

    OpenAIRE

    Siu Loon Hoe

    2007-01-01

    Organisational learning has over the years been subject of much study by scholars and managers. In the process, the organisational learning concept has been linked to many other knowledge concepts such as individual learning, learning organisation, and knowledge management. This paper draws from existing literature in organisational behaviour, human resource management, marketing, and information management, to further develop the conceptual links between organisational learning and these kno...

  2. High-resolution mapping of the spatial organization of a bacterial chromosome.

    Science.gov (United States)

    Le, Tung B K; Imakaev, Maxim V; Mirny, Leonid A; Laub, Michael T

    2013-11-01

    Chromosomes must be highly compacted and organized within cells, but how this is achieved in vivo remains poorly understood. We report the use of chromosome conformation capture coupled with deep sequencing (Hi-C) to map the structure of bacterial chromosomes. Analysis of Hi-C data and polymer modeling indicates that the Caulobacter crescentus chromosome consists of multiple, largely independent spatial domains that are probably composed of supercoiled plectonemes arrayed into a bottle brush-like fiber. These domains are stable throughout the cell cycle and are reestablished concomitantly with DNA replication. We provide evidence that domain boundaries are established by highly expressed genes and the formation of plectoneme-free regions, whereas the histone-like protein HU and SMC (structural maintenance of chromosomes) promote short-range compaction and the colinearity of chromosomal arms, respectively. Collectively, our results reveal general principles for the organization and structure of chromosomes in vivo. PMID:24158908

  3. Effect of workplace innovation on organisational performance and sickness absence

    NARCIS (Netherlands)

    Oeij, P.R.A.; Vaas, F.

    2016-01-01

    Workplace innovation is an organisational capability, defined as a strategic renewal in organising and organisational behaviour. This capability may consist of four resources: strategic orientation, product-market improvement, flexible work and organising smarter. Workplace innovation is theoretical

  4. An organisational perspective on professionals' learning

    DEFF Research Database (Denmark)

    Elkjær, Bente; Brandi, Ulrik

    2014-01-01

    that they are connected with. These are a behavioural, a cognitive and a practice-based perspective on organisational learning respectively. We propose that these lenses on organisations and learning may help us see professionals’ learning as contextualised in both their work practices and their places of work, i......The purpose and contribution of our chapter is to provide a vision for professionals’ learning in the light of the field of organisational learning, and through this lens to incorporate various understandings of the organisational dynamics that professionals work and learn in and through. Inspired...... by some of the founding fathers of the field of organisational learning, Chris Argyris and Donald Schön (Argyris & Schön, 1978; 1996: 3), we ask: “what is an organisation that professionals may learn?” We answer this question by introducing three understandings of organisations and the learning theories...

  5. Chimpanzee chromosome 12 is homologous to human chromosome 2q

    Energy Technology Data Exchange (ETDEWEB)

    Sun, N. C.; Sun, C. R.Y.; Ho, T.

    1977-01-01

    Most of the 46 human chromosomes find their counterparts in the 48 chimpanzee chromosomes except for chromosome 2 which has been hypothesized to have been derived from a centric fusion of two chimpanzee acrocentric chromosomes. These two chromosomes correspond to the human chromosomes 2p and 2g. This conclusion is based primarily on chromosome banding techniques, and the somatic cell hybridization technique has also been used. (HLW)

  6. Effect of weightlessness on colloidal particle transport and segregation in self-organising microtubule preparations.

    Science.gov (United States)

    Tabony, James; Rigotti, Nathalie; Glade, Nicolas; Cortès, Sandra

    2007-05-01

    Weightlessness is known to effect cellular functions by as yet undetermined processes. Many experiments indicate a role of the cytoskeleton and microtubules. Under appropriate conditions in vitro microtubule preparations behave as a complex system that self-organises by a combination of reaction and diffusion. This process also results in the collective transport and organisation of any colloidal particles present. In large centimetre-sized samples, self-organisation does not occur when samples are exposed to a brief early period of weightlessness. Here, we report both space-flight and ground-based (clinorotation) experiments on the effect of weightlessness on the transport and segregation of colloidal particles and chromosomes. In centimetre-sized containers, both methods show that a brief initial period of weightlessness strongly inhibits particle transport. In miniature cell-sized containers under normal gravity conditions, the particle transport that self-organisation causes results in their accumulation into segregated regions of high and low particle density. The gravity dependence of this behaviour is strongly shape dependent. In square wells, neither self-organisation nor particle transport and segregation occur under conditions of weightlessness. On the contrary, in rectangular canals, both phenomena are largely unaffected by weightlessness. These observations suggest, depending on factors such as cell and embryo shape, that major biological functions associated with microtubule driven particle transport and organisation might be strongly perturbed by weightlessness.

  7. The terminal region of the E. coli chromosome localises at the periphery of the nucleoid

    Directory of Open Access Journals (Sweden)

    Stouf Mathieu

    2011-02-01

    Full Text Available Abstract Background Bacterial chromosomes are organised into a compact and dynamic structures termed nucleoids. Cytological studies in model rod-shaped bacteria show that the different regions of the chromosome display distinct and specific sub-cellular positioning and choreographies during the course of the cell cycle. The localisation of chromosome loci along the length of the cell has been described. However, positioning of loci across the width of the cell has not been determined. Results Here, we show that it is possible to assess the mean positioning of chromosomal loci across the width of the cell using two-dimension images from wide-field fluorescence microscopy. Observed apparent distributions of fluorescent-tagged loci of the E. coli chromosome along the cell diameter were compared with simulated distributions calculated using a range of cell width positioning models. Using this method, we detected the migration of chromosome loci towards the cell periphery induced by production of the bacteriophage T4 Ndd protein. In the absence of Ndd production, loci outside the replication terminus were located either randomly along the nucleoid width or towards the cell centre whereas loci inside the replication terminus were located at the periphery of the nucleoid in contrast to other loci. Conclusions Our approach allows to reliably observing the positioning of chromosome loci along the width of E. coli cells. The terminal region of the chromosome is preferentially located at the periphery of the nucleoid consistent with its specific roles in chromosome organisation and dynamics.

  8. Coordination Processes in International Organisations

    DEFF Research Database (Denmark)

    Nedergaard, Peter

    2008-01-01

    The EU is not a member of the International Labour Organisation (ILO), but relatively elaborate EU coordination takes place anyway. This paper addresses two research questions: 1) How is it possible to evaluate the coordination of the EU in its specific observable configuration in the ILO?, and 2...... to coordinate relatively elaborate agreements due to the strength of its coordination as far as professional or technical and political activities (excepting the ILO budget) are concerned. In other more clear-cut or 'simple' policy areas such as the ILO budget, the EU coordination is weak: this contrast...

  9. The Precarious Prokaryotic Chromosome

    OpenAIRE

    Kuzminov, Andrei

    2014-01-01

    Evolutionary selection for optimal genome preservation, replication, and expression should yield similar chromosome organizations in any type of cells. And yet, the chromosome organization is surprisingly different between eukaryotes and prokaryotes. The nuclear versus cytoplasmic accommodation of genetic material accounts for the distinct eukaryotic and prokaryotic modes of genome evolution, but it falls short of explaining the differences in the chromosome organization. I propose that the t...

  10. Realising the organisational learning opportunities

    International Nuclear Information System (INIS)

    An aspect of proactive safety management is learning lessons from unforeseen events. As BNFL has expanded and extended its nuclear services to many more sites, the potential for organisational learning has grown, but sharing through informal networking has become progressively harder. This potential problem has been solved by implementing formalised company-wide arrangements to turn incidents and accidents into organisational learning opportunities through a system called 'Learning from Experience' (LFE). LFE enables event causes and corrective actions to be identified and shared across all BNFL's sites, initially in the UK but ultimately throughout the world. The result is prevention of events having similar causes, and development of a learning culture which breaks down the barriers to adopting best practice'. Key aspects of the system are: Applying root cause analysis to all significant events; Logging all events, their causes and corrective actions onto a Company-wide database; Screening the database regularly by locally appointed Feedback Co-ordinators trained in identifying learning opportunities and knowledgeable of their own business area, and; Placing and tracking actions to prevent similar events at local Event Review Meetings. The paper describes the implementation and initial experience in operation of the LFE system, which is seen as a significant step towards becoming an expanding and learning company with no accidents or incidents. (author)

  11. Ring chromosome 13

    DEFF Research Database (Denmark)

    Brandt, C A; Hertz, Jens Michael; Petersen, M B;

    1992-01-01

    A stillborn male child with anencephaly and multiple malformations was found to have the karyotype 46,XY,r(13) (p11q21.1). The breakpoint at 13q21.1, determined by high resolution banding, is the most proximal breakpoint ever reported in patients with ring chromosome 13. In situ hybridisation...... with the probe L1.26 confirmed the derivation from chromosome 13 and DNA polymorphism analysis showed maternal origin of the ring chromosome. Our results, together with a review of previous reports of cases with ring chromosome 13 with identified breakpoints, could neither support the theory of distinct clinical...

  12. Business continuity management in international organisations.

    Science.gov (United States)

    Adamou, Christel

    2014-01-01

    In the area of business continuity management, a preliminary review of the literature reveals extensive knowledge, expertise and experience concerning organisations in the private and public sectors. It is interesting to note, however, that there is little literature about business continuity management in international organisations, although these entities are complex and particularly prone to threats. This apparent absence of literature suggests that business continuity management has not yet hit the agenda of international organisations. In recent years, member states have encouraged senior management to design and implement business continuity strategies to minimise the mishandling of an internal crisis and build organisational resilience, but very few of them have actually been able to design and implement comprehensive business continuity programmes. Based on actual experience working in international organisations, this paper outlines some of the challenges faced by international organisations in developing and implementing business continuity activities and attempts to make suggestions for further improvement. PMID:24578023

  13. The institutional dynamics of voluntary organisations

    DEFF Research Database (Denmark)

    Aagaard, Peter

    to the organisation may give volunteers special opportunities to choose, not only where and when, but also how they will perform. But in praxis the volunteer's ability to perform is structured by the institutional settings of the specific voluntary organisation as well as the organisational field of voluntary......What features of institutional change do voluntary organisations contain? This question is debated in the civil society literature, but often under different headlines, like social entrepreneurship or social movement theory. The question of voluntarism is often not taken into account. This paper...... organisations. I establish a theoretical frame of institutional dynamic, build primarily on J.G. March's theory on exploration and exploitation. I focus on two organisational arrangements drawn from the theory: The degree of strategic decision-making and the degree of diversity among the volunteers. I use...

  14. Business continuity management in international organisations.

    Science.gov (United States)

    Adamou, Christel

    2014-01-01

    In the area of business continuity management, a preliminary review of the literature reveals extensive knowledge, expertise and experience concerning organisations in the private and public sectors. It is interesting to note, however, that there is little literature about business continuity management in international organisations, although these entities are complex and particularly prone to threats. This apparent absence of literature suggests that business continuity management has not yet hit the agenda of international organisations. In recent years, member states have encouraged senior management to design and implement business continuity strategies to minimise the mishandling of an internal crisis and build organisational resilience, but very few of them have actually been able to design and implement comprehensive business continuity programmes. Based on actual experience working in international organisations, this paper outlines some of the challenges faced by international organisations in developing and implementing business continuity activities and attempts to make suggestions for further improvement.

  15. Organisational Change, Health and the Labour Market

    DEFF Research Database (Denmark)

    Bhatti, Yosef; Gørtz, Mette; Holm Pedersen, Lene

    This research examines the effects of organisational change on employee health and labour market outcomes. Previous studies looking into organisational change in the private sector indicate that the larger the size and depth of organisational change, the larger the detrimental consequences...... change. And fourth, we have access to objective measures of health outcomes from register data. The results show that the effects of organisational change on health outcomes are limited and heterogeneous. Change per se does not necessarily lead to worsened employee outcomes. However, the degree...... of the change seems to matter. The smaller share an old organisation constitutes of the new organisation, the larger the effect is on health outcomes for its employees....

  16. Effective Diagnosis in Organisation Change Management

    Directory of Open Access Journals (Sweden)

    Ahmed Hassin

    2014-09-01

    Full Text Available Organisations are part of interactive and dynamic environments and modern organisations face considerable pressure to meet or exceed customer/ beneficiary’s and stakeholders’ expectations. The key to establishing effective change and transformation in organisations lies in the early stages of assessment and diagnosis: if diagnosis is wrong, treatment will be ineffective. An organisation development programme should be based on a sound analysis of relevant data about the problem situation, and during the diagnosis process, it is important to look at both the environment and organisation. The OD practitioner’s choice of a certain diagnosis method or model or a combination of methods depends on the context, type of problem and organisation.

  17. The gendered organisation : A positive critique

    OpenAIRE

    Hearn, Jeff; Parkin, Wendy

    2003-01-01

    In this article we go back to basics – to the very idea of the gendered organisation. Probably all researchers on gender and diversity in the workplace have some notion, however implicit, of the gendered organisation. This applies in both empirical research and more general theoretical analysis of organisations and management. Our task here is to assist in making explicit what those assumptions may be and help us to take a critical look at how we understand and conceptualise ‘the ...

  18. Managing evaluation : a community arts organisation's perspective.

    OpenAIRE

    Swan, P.J.; Atkinson, S.

    2012-01-01

    Background: Arts and health organisations must increasingly provide measurable evidence of impact to stakeholders, which can pose both logistical and ideological challenges. This article examines the relationship between the ethos of an arts and health organisation and external demands for evaluation. Methods: Research involved an ethnographic engagement where the first author worked closely with the organisation for a year. In addition to informal discussions, 20 semi-structured int...

  19. Main Features of SMEs Organisation System

    OpenAIRE

    Ovidiu NICOLESCU

    2009-01-01

    SMEs present many particularities which influence to a large extend their functionality and performance. A very deep investigation of more than 60 SMEs has revealed what are the major variables having influence on SMEs organisation and the main features of organisation system. In the first part the paper indicate the variables influencing the SMEs. In the second part there are presented the nine main features of SMEs organisation system.

  20. Organisational Learning and Konwledge Management within NGO

    OpenAIRE

    Neagu Olimpia

    2013-01-01

    NGOs are learning as well as knowledge- intensiv organisations. They are creating social value in the community where they act as social change agents, by creating, sharing and using knowledge. As components of the new, knowledge-based society, their organisational processes are marked by learning and knowledge. The paper aims to propose a conceptual model of the link between organisational learning and knowledge management within NGOs

  1. Meaningful work, work engagement and organisational commitment

    OpenAIRE

    Madelyn Geldenhuys; Karolina Łaba; Cornelia M. Venter

    2014-01-01

    Orientation: Meaningful work can yield benefits for organisations and lead to positive work outcomes such as satisfied, engaged and committed employees, individual and organisational fulfilment, productivity, retention and loyalty.Research purpose: The aim of the study was to investigate the relationships amongst psychological meaningfulness, work engagement and organisational commitment and to test for a possible mediation effect of work engagement on the relationship between psychological ...

  2. Learning Organisation and the Process of Regionalisation

    OpenAIRE

    Gustavsen, Bjørn

    2006-01-01

    "Concepts like Taylorism, lean production and learning organisation draw attention to the point that work organisation can appear in different forms and it is generally recognised that different conditions tend to produce different forms. Still, there is a tendency to underplay how different these generative conditions are. In this article the issue of learning organisation is placed in focus, drawing upon experiences from Scandinavian workplace development programmes. These...

  3. Impact of New Technologies on Public Organisations

    OpenAIRE

    Matei, Lucica

    2009-01-01

    ICTs impact is important on organisational structure and organisational culture.IT offers options for the design of organisational culture,the interaction by means of new modalities ,joint fonctions and objectives,faciliting the set up of working groups with a determined term,as well as communication on large areas. The use of effective IT could provide an attractive work environment ,and could motivate the employees by means of job enrichment. ICTs represent a catalyst for the public...

  4. Social media and small non profit organisations

    OpenAIRE

    Gulyás, A.

    2016-01-01

    This project sought to explore patterns of and views about social media adoption among small non-profit organisations and to identify mechanisms that could effectively support these organisations with their social media use. The following findings emerged from the study: • Social media are now part of the organisational infrastructure of small non-profits and the main ways in which they communicate with the public • Key variables that influence social media adoption among small no...

  5. Rheumatoid arthritis and cryptogenic organising pneumonitis.

    Science.gov (United States)

    Rees, J H; Woodhead, M A; Sheppard, M N; du Bois, R M

    1991-05-01

    We describe three patients with rheumatoid arthritis who presented with non-specific pulmonary symptoms, a restrictive defect in lung function and bilateral changes on chest radiograph. Lung histology showed characteristic features of cryptogenic organising pneumonitis and treatment with steroids produced significant improvement. The clinical and laboratory features of cryptogenic organising pneumonitis (otherwise known as bronchiolitis obliterans organising pneumonia, 'BOOP') are discussed and compared with those of bronchiolitis obliterans with which the condition should not be confused. Cryptogenic organising pneumonitis should be considered as one of the pulmonary manifestations of rheumatoid arthritis, but lung biopsy is essential to make the diagnosis.

  6. Evaluation of organisational culture and nurse burnout.

    Science.gov (United States)

    Watts, Jenny; Robertson, Noelle; Winter, Rachel; Leeson, David

    2013-10-01

    A survey of nurses working with older adults across three NHS trusts was conducted to explore how perceptions of the workplace affect nurse wellbeing. Standardised validated measures were used to assess burnout, perceived organisational support and organisational culture. Significant associations were found between innovative organisational culture and nurses' sense of personal accomplishment, which reduce the likelihood of burnout. Multiple regression showed experience of burnout to be predicted by the nature of organisational culture. It seems therefore that nurses' wellbeing may be affected by their perceptions of the working environment. Applications of this knowledge and suggestions for future research are discussed. PMID:24063341

  7. EMPLOYEE LEARNING AND DEVELOPMENT IN ORGANISATIONS

    Directory of Open Access Journals (Sweden)

    VNOUČKOVÁ, Lucie

    2013-09-01

    Full Text Available The primary goal of all organisations is efficiency of human resources. Therefore activities as HR controlling, performance management but also cutting costs are the main theme. Current organisations need to monitor human resources to keep their competitiveness. Thus paper describes the key factor of organisational efficiency - employee education, talent management and the necessity to retain skilled employees. The aim of the paper is to reveal the current approach in organisations to education and learning based on primary survey of employees. The data were collected using quantitative primary survey in Czech organisations across sectors. The questionnaire was compiled based on the theoretical background. The paper has been processed based on the analysis of secondary sources, outcome synthesis and the evaluation of results of a questionnaire survey. The data were analysed using descriptive statistic, correlation analysis and factor analysis. The SPSS programme was used for the analyses. The outcomes were categorized and the analyses revealed the main factors affecting organisational approach to employee learning and development. The results identify three possible approaches in organisations to employee learning and development. The first type of organisations educates employees by their own rules, second type does not support education of employees in any way, it is only an interest of employees themselves and thirdly knowledgeable employees were identified as those employees do as much as possible to learn and grow and they choose job position in order to develop constantly. The results can be taken into account in further analysis and in organisation of adult education.

  8. Evaluation of organisational culture and nurse burnout.

    Science.gov (United States)

    Watts, Jenny; Robertson, Noelle; Winter, Rachel; Leeson, David

    2013-10-01

    A survey of nurses working with older adults across three NHS trusts was conducted to explore how perceptions of the workplace affect nurse wellbeing. Standardised validated measures were used to assess burnout, perceived organisational support and organisational culture. Significant associations were found between innovative organisational culture and nurses' sense of personal accomplishment, which reduce the likelihood of burnout. Multiple regression showed experience of burnout to be predicted by the nature of organisational culture. It seems therefore that nurses' wellbeing may be affected by their perceptions of the working environment. Applications of this knowledge and suggestions for future research are discussed.

  9. IAU Public Astronomical Organisations Network

    Science.gov (United States)

    Canas, Lina; Cheung, Sze Leung

    2015-08-01

    The Office for Astronomy Outreach has devoted intensive means to create and support a global network of public astronomical organisations around the world. Focused on bringing established and newly formed amateur astronomy organizations together, providing communications channels and platforms for disseminating news to the global community and the sharing of best practices and resources among these associations around the world. In establishing the importance that these organizations have for the dissemination of activities globally and acting as key participants in IAU various campaigns social media has played a key role in keeping this network engaged and connected. Here we discuss the implementation process of maintaining this extensive network, the processing and gathering of information and the interactions between local active members at a national and international level.

  10. Simultaneous Attacks by Terrorist Organisations

    Directory of Open Access Journals (Sweden)

    Kathleen Deloughery

    2013-12-01

    Full Text Available While terror attacks that are a part of a coordinated effort receive attention in the popular media, they have not received much attention in the academic literature. The decision to carry out simultaneous attacks should be examined as one of the choices a terrorist organisation makes about the method of attack. Determining the impact of simultaneous attacks vis-à-vis a single attack can explain why groups would use this method. Up to one quarter of all attacks coded in two major databases, GTD and ITERATE, may be part of a simultaneous campaign. Empirical analysis shows simultaneous attacks are more likely to be successful and cause more fatalities, though not in a one-to-one fashion. These results underline the importance of considering simultaneous attacks in empirical analysis.

  11. The Immigrant Organising Process : Turkish Organisations in Amsterdam and Berlin and Surinamese Organisations in Amsterdam, 1960-2000

    NARCIS (Netherlands)

    Vermeulen, Floris

    2006-01-01

    This study focuses on the emergence and persistence of immigrant organisations in host societies. The relevance of immigrant organisations for both the host society and the immigrants themselves has been effectively demonstrated in many different studies. However, the question why immigrant organisa

  12. Organisational values as "attractors of chaos": An emerging cultural change to manage organisational complexity

    OpenAIRE

    Shimon L. Dolan; Garc??a, Salvador; Diegoli, Samantha; Auerbach, Alan

    2000-01-01

    Business organisations are excellent representations of what in physics and mathematics are designated "chaotic" systems. Because a culture of innovation will be vital for organisational survival in the 21st century, the present paper proposes that viewing organisations in terms of "complexity theory" may assist leaders in fine-tuning managerial philosophies that provide orderly management emphasizing stability within a culture of organised chaos, for it is on ...

  13. Electochemical detection of chromosome translocation

    DEFF Research Database (Denmark)

    Kwasny, Dorota; Dimaki, Maria; Silahtaroglu, Asli;

    2014-01-01

    Cytogenetics is a study of the cell structure with a main focus on chromosomes content and their structure. Chromosome abnormalities, such as translocations may cause various genetic disorders and heametological malignancies. Chromosome translocations are structural rearrangements of two chromoso...

  14. 'Organised' cervical screening 45 years on: How consistent are organised screening practices?

    Science.gov (United States)

    Williams, Jane H; Carter, Stacy M; Rychetnik, Lucie

    2014-11-01

    Organised screening programmes have been remarkably successful in reducing incidence and mortality from cervical cancer, while opportunistic screening varies in its effectiveness. Experts recommend that cervical screening or HPV testing be carried out only in the context of an organised programme. We sought to answer the following study questions: What does it mean for a cervical screening programme to be organised? Is there a place for opportunistic screening (in an organised programme)? We reviewed 154 peer-reviewed papers on organised and opportunistic approaches to cervical screening published between 1970 and 2014 to understand how the term 'organised' is used, formally and in practice. We found that despite broad recognition of a prescriptive definition of organisation, in practice the meaning of organisation is much less clear. Our review revealed descriptions of organised programmes that differ significantly from prescribed norms and from each other, and a variety of ways that opportunistic and organised programmes intersect. We describe the breadth of the variation in cervical cancer screening programmes and examine the relationships and overlaps between organised and opportunistic screening. Implications emerging from the review include the need to better understand the breadth of organisation in practice, the drivers and impacts of opportunistic screening and the impact of opportunistic screening on population programme outcomes. Appreciation of the complexity of cervical screening programmes will benefit both screeners and women as programmes are changed to reflect a partially vaccinated population, new evidence and new technologies.

  15. Managing intangible assets: An essay on organising contemporary organisations based upon identity, competencies and networks

    NARCIS (Netherlands)

    Schoemaker, M.J.R.; Jonker, J.

    2005-01-01

    - Purpose – To develop a concept of managing intangible assets in contemporary organisations. Insight is given into the rise of the network organisation and the importance of talent, social capital and identity in this kind of organisation. - Design/methodology/approach – This paper develops a conce

  16. Case studies on age-management in organisations: report on organisational case studies

    NARCIS (Netherlands)

    Punte, E.; Conen, W.S.; Schippers, J.; Henkens, C.J.I.M.

    2011-01-01

    The acquisition of case studies was thwarted by the economic crisis and the feeling of being ‘over-researched’ by potential organisations. Although organisations in some sectors (e.g. chemical manufacturing) reported the worst part of the economic crisis was behind, many organisations indicated gett

  17. Exploring the Interconnectedness among Strategy Development, Shared Mental Models, Organisational Learning and Organisational Change

    Science.gov (United States)

    Malan, Renee

    2011-01-01

    The cognitive psychological processes related to learning and change behaviour are factors that impact on organisational strategy development. Strategy development is dependent on strategic thinking that is reciprocally influenced by shared mental models, organisational learning and organisational change. Although strategy development, shared…

  18. Clinical impact of ABL1 kinase domain mutations and IKZF1 deletion in adults under age 60 with Philadelphia chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL): molecular analysis of CALGB (Alliance) 10001 and 9665.

    Science.gov (United States)

    DeBoer, Rebecca; Koval, Gregory; Mulkey, Flora; Wetzler, Meir; Devine, Steven; Marcucci, Guido; Stone, Richard M; Larson, Richard A; Bloomfield, Clara D; Geyer, Susan; Mullighan, Charles G; Stock, Wendy

    2016-10-01

    Recent studies have identified oncogenic lesions in Philadelphia chromosome-positive (Ph+)  acute lymphoblastic leukemia (ALL) and ABL1 kinase mutations that confer resistance to tyrosine kinase inhibitors. We sought to determine the prevalence and clinical impact of these lesions in patients on CALGB 10001, a previously reported Phase II study of imatinib, chemotherapy, and hematopoietic cell transplant in adult Ph + ALL. Of the 58 enrolled, 22 relapsed. By direct sequencing, an ABL1 kinase mutation known to induce imatinib resistance was present at relapse in 13 of 20. Using quantitative PCR assays, the mutations were detectable at diagnosis or early during treatment in most (62%) relapsed patients. Aberrations in IKZF1, CDKN2A/B, and PAX5 were assessed in 28 samples using SNP arrays and genomic DNA sequencing. Of these, 22 (79%) had IKZF1 deletion. The combination of IKZF1 deletion and p210 BCR-ABL1 (p < 0.0001), high white blood cell count (p = 0.021), and minimal residual disease (p = 0.013) were associated with worse disease-free survival. PMID:26892479

  19. Organisational Learning and Employees' Intrinsic Motivation

    Science.gov (United States)

    Remedios, Richard; Boreham, Nick

    2004-01-01

    This study examined the effects of organisational learning initiatives on employee motivation. Four initiatives consistent with theories of organisational learning were a priori ranked in terms of concepts that underpin intrinsic-motivation theory. Eighteen employees in a UK petrochemical company were interviewed to ascertain their experiences of…

  20. The "State of Art" of Organisational Blogging

    Science.gov (United States)

    Baxter, Gavin J.; Connolly, Thomas M.

    2013-01-01

    Purpose: The aim of this paper is to provide an overview of the "state of art" of organisational blogging. It also aims to provide a critical review of the literature on organisational blogging and propose recommendations on how to advance the subject area in terms of academic research. Design/methodology/approach: A systematic literature review…

  1. Impacts of IST on firms and organisations

    DEFF Research Database (Denmark)

    Jørgensen, Ulrik

    2003-01-01

    A review of socio-economic research on the impact and role of IST in firms and organisations. The study is carried out in ESTO for IPTS.......A review of socio-economic research on the impact and role of IST in firms and organisations. The study is carried out in ESTO for IPTS....

  2. Relating structure and dynamics in organisation models

    NARCIS (Netherlands)

    Jonkers, C.M.; Treur, J.

    2008-01-01

    To understand how an organisational structure relates to dynamics is an interesting fundamental challenge in the area of social modelling. Specifications of organisational structure usually have a diagrammatic form that abstracts from more detailed dynamics. Dynamic properties of agent systems, on t

  3. International Organisations and Transnational Education Policy

    Science.gov (United States)

    Moutsios, Stavros

    2009-01-01

    This paper focuses on the World Bank/IMF (International Monetary Fund), the OECD (Organisation for Economic Cooperation and Development) and the WTO (World Trade Organisation) as institutions of transnational policy making. They are all at present making education policies which are decisively shaping current directions and developments in…

  4. The Shanghai Co-operation Organisation

    DEFF Research Database (Denmark)

    Hansen, Flemming Splidsboel

    New regional organisations are always interesting to follow as they will tell us more about the things that make or break this type of co-operation. Originally established as “The Shanghai Five” in 1996, The Shanghai Co-operation Organisation (SCO) has been successful at reducing tension between ...

  5. Meaningful work, work engagement and organisational commitment

    Directory of Open Access Journals (Sweden)

    Madelyn Geldenhuys

    2014-02-01

    Full Text Available Orientation: Meaningful work can yield benefits for organisations and lead to positive work outcomes such as satisfied, engaged and committed employees, individual and organisational fulfilment, productivity, retention and loyalty.Research purpose: The aim of the study was to investigate the relationships amongst psychological meaningfulness, work engagement and organisational commitment and to test for a possible mediation effect of work engagement on the relationship between psychological meaningfulness and organisational commitment.Motivation for the study: Managers have to rethink ways of improving productivity and performance at work, due to the diverse, and in some instances escalating, needs of employees (e.g. financial support to uphold their interest in and enjoyment of working.Research approach, design and method: A quantitative approach was employed to gather the data for the study, utilising a cross-sectional survey design. The sample (n = 415 consisted of working employees from various companies and positions in Gauteng, South Africa.Main findings: The results confirmed a positive relationship between psychological meaningfulness, work engagement and organisational commitment. Further, psychological meaningfulness predicts work engagement, whilst psychological meaningfulness and work engagement predict organisational commitment.Practical/managerial implications: Employers identifying their employees’ commitment patterns and mapping out strategies for enhancing those that are relevant to organisational goals will yield positive work outcomes (e.g. employees who are creative, seek growth or challenges for themselves.Contribution/value-add: This study contributes to the literature through highlighting the impact that meaningful work has on sustaining employee commitment to the organisation.

  6. Agreeing on role adoption in open organisations

    NARCIS (Netherlands)

    Aldewereld, H.; Dignum, V.; Jonker, C.M.; Van Riemsdijk, M.B.

    2012-01-01

    The organisational specification of a multi-agent system supports agents’ effectiveness in attaining their purpose, or prevent certain undesired behaviour from occurring. This requires that agents are able to find out about the organisational purpose and description and decide on its appropriateness

  7. European Perspectives on the Learning Organisation

    Science.gov (United States)

    Nyhan, Barry; Cressey, Peter; Tomassini, Massimo; Kelleher, Michael; Poell, Rob

    2004-01-01

    This paper, based on a publication entitled "Facing up to the Learning Organisation Challenge," published in April 2003, provides an overview of the main questions emerging from recent European research projects related to the topic of the learning organisation. The rationale for focusing on this topic is the belief that the European Union goals…

  8. Constraint-based Modelling of Organisations

    NARCIS (Netherlands)

    Viara, Popova; Alexei, Sharpanskykh

    2008-01-01

    Modern organisations are characterised by a great variety of forms and often involve many actors with diverse goals, performing a wide range of tasks in changing environmental conditions. Due to high complexity, mistakes and inconsistencies are not rare in organisations. To provide better insights i

  9. Identity Building in Organisations: Proactive Capability Development

    DEFF Research Database (Denmark)

    Rasmussen, Lauge Baungaard

    2002-01-01

    Identity building in organisations is often viewed as legitimacy of value systems of the organisation. Based on empirical studies the task of this article is to argue that such a legitimacy approach risks failing in the longer perspective, if the proactive capability development is neglected. The...

  10. Organising Quality Function in Research and Development

    Directory of Open Access Journals (Sweden)

    Rajendra Prasad

    2006-01-01

    Full Text Available Quality function may be summarised as quality generation, quality control, and qualityassurance. The quality generation and quality control, because of their fuactional nature, arecomparatively easy to organise, however, the challenge is to organise quality assurance in theorganisation. It is the challenge because quality assurance should seamlessly merge with theR&D process. In future, the organisations, whether real or virtual, are going to bepredominantly, research and development (R&D oriented rather than pure manufacturing orservice providers. However, the nature and intensity of the R&D may differ. It could be fromsimple improvement to innovation, to exploitation of inventions.Organising quality function in R&D means organising its various dimensions against thestipulated criteria such as the realisation of the quality system; realisation of quality duringproduct development, including system engineering; staffing of quality generation, quality control,and quality assurance; balancing of the quality function; harnessing of project quality, functionalquality, staff quality and line quality; application of the RHR principle; exploitation of the toolsof organising, democratic organisation; and so on. The basic purpose of organising the qualityfunction is to inculcate the sense of pride in quality among the scientists so that they feelpassionate about it. It is the passion and love for quality that generates, sustains, and maintainsquality.

  11. The Self-Organising Fuzzy Controller

    DEFF Research Database (Denmark)

    Jantzen, Jan

    1998-01-01

    A marginally stable test system, with a large dead time and an integrator, is stabilised by a self-organising fuzzy controller in a simulation study. It acts as a case study, to explain the self-organising controller to engineering students. The paper is one of a series of tutorial papers...... for a course in fuzzy control....

  12. E-Learning in Small Organisations

    Science.gov (United States)

    Sambrook, Sally

    2003-01-01

    This paper focuses on the existing and potential role of electronic learning in small and medium-sized organisations (SMEs). Innovations in information and communication technologies (ICTs) could create new forms of learning, particularly appealing to small organisations, to overcome traditional barriers such as lack of financial resources, time,…

  13. Organising European technical documentation to avoid duplication.

    Science.gov (United States)

    Donawa, Maria

    2006-04-01

    The development of comprehensive accurate and well-organised technical documentation that demonstrates compliance with regulatory requirements is a resource-intensive, but critically important activity for medical device manufacturers. This article discusses guidance documents and method of organising technical documentation that may help avoid costly and time-consuming duplication. PMID:16736662

  14. Achieving Organisational Change through Values Alignment

    Science.gov (United States)

    Branson, Christopher M.

    2008-01-01

    Purpose: The purpose of this paper is to, first, establish the interdependency between the successful achievement of organisational change and the attainment of values alignment within an organisation's culture and then, second, to describe an effective means for attaining such values alignment. Design/methodology/approach: Literature from the…

  15. A Model of Project and Organisational Dynamics

    Directory of Open Access Journals (Sweden)

    Jenny Leonard

    2012-04-01

    Full Text Available The strategic, transformational nature of many information systems projects is now widely understood. Large-scale implementations of systems are known to require significant management of organisational change in order to be successful. Moreover, projects are rarely executed in isolation – most organisations have a large programme of projects being implemented at any one time. However, project and value management methodologies provide ad hoc definitions of the relationship between a project and its environment. This limits the ability of an organisation to manage the larger dynamics between projects and organisations, over time, and between projects. The contribution of this paper, therefore, is to use literature on organisational theory to provide a more systematic understanding of this area. The organisational facilitators required to obtain value from a project are categorised, and the processes required to develop those facilitators are defined. This formalisation facilitates generalisation between projects and highlights any time and path dependencies required in developing organisational facilitators. The model therefore has the potential to contribute to the development of IS project management theory within dynamic organisational contexts. Six cases illustrate how this model could be used.

  16. Facilitating "Organisational Learning" in a "Learning Institution"

    Science.gov (United States)

    Lawler, Alan; Sillitoe, James

    2013-01-01

    The term "organisational learning" was popularised by Peter Senge in "The Fifth Discipline", his seminal book from 1990. Since then, the term has become widely accepted among those interested in organisational learning and change management. However, partly due to the somewhat ambiguous situation which arises in a university…

  17. Building Innovative Vocational Education and Training Organisations

    Science.gov (United States)

    Callan, Victor

    2004-01-01

    Highly innovative organisations demonstrate six key characteristics. They create learning cultures which promote innovation as a core organisational capability; employ leaders who are "failure-tolerant"; identify innovators; reward people who propose innovative ideas; use partnerships; and, promote innovation through teams. This report analyses…

  18. The organisational aspect of faculty development

    DEFF Research Database (Denmark)

    Kolmos, Anette; Gynnild, Vidar; Roxå, Torgny

    2004-01-01

    The article points out the faculty centres ought to be more conscious in their organisational strategies and get to managements support when working on pedagogical changes.......The article points out the faculty centres ought to be more conscious in their organisational strategies and get to managements support when working on pedagogical changes....

  19. Organisational IT Strategy Development using GDSS

    DEFF Research Database (Denmark)

    Sørensen, Lene

    1999-01-01

    IT strategy development in organisations is a complex task for which there is an immediate need for methodological support to be able to make satisfying decisions. Group Decision Support Systems (GDSS) is one line of supporting the process of group decision making and supporting IT strategy...... to support development of organisational IT strategies....

  20. New challenges for the internal safety organisation

    DEFF Research Database (Denmark)

    Hasle, Peter; Jensen, Per Langå

    2003-01-01

    Research from several countries indicates that the internal health and safety organisation in most companies is placed in an appendix position. Introduc-tion of learning is a possibility for the development of a stronger and more ef-fective health and safety organisation. This approach has been...... studied in a Danish network project with 11 companies....

  1. Organisational Blogs: Benefits and Challenges of Implementation

    Science.gov (United States)

    Baxter, Gavin J.; Connolly, Thomas M.; Stansfield, Mark H.

    2010-01-01

    Purpose: The purpose of this paper is to identify the theoretical link between blogs and organisational learning. It aims to provide a set of practical guidelines on how to overcome the challenges of implementing an organisational blog. Design/methodology/approach: A literature review will be used to examine blogs and their association towards…

  2. Specific patterns of gene space organisation revealed in wheat by using the combination of barley and wheat genomic resources

    Directory of Open Access Journals (Sweden)

    Waugh Robbie

    2010-12-01

    Full Text Available Abstract Background Because of its size, allohexaploid nature and high repeat content, the wheat genome has always been perceived as too complex for efficient molecular studies. We recently constructed the first physical map of a wheat chromosome (3B. However gene mapping is still laborious in wheat because of high redundancy between the three homoeologous genomes. In contrast, in the closely related diploid species, barley, numerous gene-based markers have been developed. This study aims at combining the unique genomic resources developed in wheat and barley to decipher the organisation of gene space on wheat chromosome 3B. Results Three dimensional pools of the minimal tiling path of wheat chromosome 3B physical map were hybridised to a barley Agilent 15K expression microarray. This led to the fine mapping of 738 barley orthologous genes on wheat chromosome 3B. In addition, comparative analyses revealed that 68% of the genes identified were syntenic between the wheat chromosome 3B and barley chromosome 3 H and 59% between wheat chromosome 3B and rice chromosome 1, together with some wheat-specific rearrangements. Finally, it indicated an increasing gradient of gene density from the centromere to the telomeres positively correlated with the number of genes clustered in islands on wheat chromosome 3B. Conclusion Our study shows that novel structural genomics resources now available in wheat and barley can be combined efficiently to overcome specific problems of genetic anchoring of physical contigs in wheat and to perform high-resolution comparative analyses with rice for deciphering the organisation of the wheat gene space.

  3. Actin-organising properties of the muscular dystrophy protein myotilin.

    Science.gov (United States)

    von Nandelstadh, Pernilla; Grönholm, Mikaela; Moza, Monica; Lamberg, Arja; Savilahti, Harri; Carpén, Olli

    2005-10-15

    Myotilin is a sarcomeric Z-disc protein that binds F-actin directly and bundles actin filaments, although it does not contain a conventional actin-binding domain. Expression of mutant myotilin leads to sarcomeric alterations in the dominantly inherited limb-girdle muscular dystrophy 1A and in myofibrillar myopathy/desmin-related myopathy. Together, with previous in vitro studies, this indicates that myotilin has an important function in the assembly and maintenance of Z-discs. This study characterises further the interaction between myotilin and actin. Functionally important regions in myotilin were identified by actin pull-down and yeast two-hybrid assays and with a novel strategy that combines in vitro DNA transposition-based peptide insertion mutagenesis with phenotype analysis in yeast cells. The shortest fragment to bind actin was the second Ig domain together with a short C-terminal sequence. Concerted action of the first and second Ig domain was, however, necessary for the functional activity of myotilin, as verified by analysis of transposon mutants, actin binding and phenotypic effect in mammalian cells. Furthermore, the Ig domains flanked with N- and C-terminal regions were needed for actin-bundling, indicating that the mere actin-binding sequence was insufficient for the actin-regulating activity. None of the four known disease-associated mutations altered the actin-organising ability. These results, together with previous studies in titin and kettin, identify the Ig domain as an actin-binding unit.

  4. Entropy Methods in Guided Self-Organisation

    Directory of Open Access Journals (Sweden)

    Mikhail Prokopenko

    2014-10-01

    Full Text Available Self-organisation occurs in natural phenomena when a spontaneous increase in order is produced by the interactions of elements of a complex system. Thermodynamically, this increase must be offset by production of entropy which, broadly speaking, can be understood as a decrease in order. Ideally, self-organisation can be used to guide the system towards a desired regime or state, while "exporting" the entropy to the system's exterior. Thus, Guided Self-Organisation (GSO attempts to harness the order-inducing potential of self-organisation for specific purposes. Not surprisingly, general methods developed to study entropy can also be applied to guided self-organisation. This special issue covers abroad diversity of GSO approaches which can be classified in three categories: information theory, intelligent agents, and collective behavior. The proposals make another step towards a unifying theory of GSO which promises to impact numerous research fields.

  5. Action research in inter-organisational networks

    DEFF Research Database (Denmark)

    Goduscheit, René Chester; Rasmussen, Erik Stavnsager; Jørgensen, Jacob Høj;

    2007-01-01

    Traditionally, the literature on action research has been aimed at intra-organisational issues. These studies have distinguished between two researcher roles: The problem-solver and the observer. This article addresses the distinct challenges of action research in inter-organisational projects....... In addition to the problem-solver and observer roles, the researcher in an inter-organisational setting can serve as a legitimiser of the project and manage to involve partners that in an ordinary business-to-business setting would not have participated. Based on an action research project in a Danish inter......-organisational network, this article discusses potential pitfalls in the legitimiser role. Lack of clarity in defining the researcher role and project ownership in relation to the funding organisation and the rest of the network can jeopardise the project and potentially the credibility of the researchers. The article...

  6. Impact of organisational change on mental health

    DEFF Research Database (Denmark)

    Grandjean Bamberger, Simon; Vinding, Anker Lund; Larsen, Anelia;

    2012-01-01

    Although limited evidence is available, organisational change is often cited as the cause of mental health problems. This paper provides an overview of the current literature regarding the impact of organisational change on mental health. A systematic search in PUBMED, PsychInfo and Web...... of Knowledge combining MeSH search terms for exposure and outcome. The criterion for inclusion was original data on exposure to organisational change with mental health problems as outcome. Both cross-sectional and longitudinal studies were included. We found in 11 out of 17 studies, an association between...... organisational change and elevated risk of mental health problems was observed, with a less provident association in the longitudinal studies. Based on the current research, this review cannot provide sufficient evidence of an association between organisational change and elevated risk of mental health problems...

  7. Domain Engineering

    Science.gov (United States)

    Bjørner, Dines

    Before software can be designed we must know its requirements. Before requirements can be expressed we must understand the domain. So it follows, from our dogma, that we must first establish precise descriptions of domains; then, from such descriptions, “derive” at least domain and interface requirements; and from those and machine requirements design the software, or, more generally, the computing systems.

  8. Sequential cloning of chromosomes

    Energy Technology Data Exchange (ETDEWEB)

    Lacks, S.A.

    1991-12-31

    A method for sequential cloning of chromosomal DNA and chromosomal DNA cloned by this method are disclosed. The method includes the selection of a target organism having a segment of chromosomal DNA to be sequentially cloned. A first DNA segment, having a first restriction enzyme site on either side. homologous to the chromosomal DNA to be sequentially cloned is isolated. A first vector product is formed by ligating the homologous segment into a suitably designed vector. The first vector product is circularly integrated into the target organism`s chromosomal DNA. The resulting integrated chromosomal DNA segment includes the homologous DNA segment at either end of the integrated vector segment. The integrated chromosomal DNA is cleaved with a second restriction enzyme and ligated to form a vector-containing plasmid, which is replicated in a host organism. The replicated plasmid is then cleaved with the first restriction enzyme. Next, a DNA segment containing the vector and a segment of DNA homologous to a distal portion of the previously isolated DNA segment is isolated. This segment is then ligated to form a plasmid which is replicated within a suitable host. This plasmid is then circularly integrated into the target chromosomal DNA. The chromosomal DNA containing the circularly integrated vector is treated with a third, retrorestriction enzyme. The cleaved DNA is ligated to give a plasmid that is used to transform a host permissive for replication of its vector. The sequential cloning process continues by repeated cycles of circular integration and excision. The excision is carried out alternately with the second and third enzymes.

  9. Topological Organization of Multi-chromosomal Regions by Firre

    Science.gov (United States)

    Hacisuleyman, Ezgi; Goff, Loyal A.; Trapnell, Cole; Williams, Adam; Henao-Mejia, Jorge; Sun, Lei; McClanahan, Patrick; Hendrickson, David G.; Sauvageau, Martin; Kelley, David R.; Morse, Michael; Engreitz, Jesse; Lander, Eric S.; Guttman, Mitch; Lodish, Harvey F.; Flavell, Richard; Raj, Arjun; Rinn, John L.

    2014-01-01

    RNA is known to be an abundant and important structural component of the nuclear matrix, including long noncoding RNAs (lncRNA). Yet the molecular identities, functional roles, and localization dynamics of lncRNAs that influence nuclear architecture remain poorly understood. Here, we describe one lncRNA, Firre, that interacts with the nuclear matrix factor hnRNPU, through a 156 bp repeating sequence and Firre localizes across a ~5 Mb domain on the X-chromosome. We further observed Firre localization across at least five distinct trans-chromosomal loci, which reside in spatial proximity to the Firre genomic locus on the X-chromosome. Both genetic deletion of the Firre locus or knockdown of hnRNPU resulted in loss of co-localization of these trans-chromosomal interacting loci. Thus, our data suggest a model in which lncRNAs such as Firre can interface with and modulate nuclear architecture across chromosomes. PMID:24463464

  10. CHROMOSOMES OF AMERICAN MARSUPIALS.

    Science.gov (United States)

    BIGGERS, J D; FRITZ, H I; HARE, W C; MCFEELY, R A

    1965-06-18

    Studies of the chromosomes of four American marsupials demonstrated that Caluromys derbianus and Marmosa mexicana have a diploid number of 14 chromosomes, and that Philander opossum and Didelphis marsupialis have a diploid number of 22. The karyotypes of C. derbianus and M. mexicana are similar, whereas those of P. opossum and D. marsupialis are dissimilar. If the 14-chromosome karyotype represents a reduction from a primitive number of 22, these observations suggest that the change has occurred independently in the American and Australasian forms.

  11. The relationship between organisational climate and employee satisfaction in a South African information and technology organisation

    Directory of Open Access Journals (Sweden)

    Monia L. Castro

    2010-03-01

    Full Text Available Orientation: Organisational climate and job satisfaction are distinct but related constructs, and both appear to influence employees’ understanding of the work environment and their level of job satisfaction.Research purpose: The objective of this study was to explore the relationship between organisational climate and job satisfaction to determine whether employees’ perceptions of the work environment influence their level of job satisfaction.Motivation for the study: Organisations are facing more challenges than ever before. These challenges are not unique to any specific organisation or industry, but affect all organisations.Organisational climate in particular is constantly challenged by changes impacting organisations today.Research design, approach and method: An organisational climate questionnaire was administered to a convenience sample of 696 employees from a population of 1453 employees working in three regions in which the organisation was operational. Confirmatory and exploratory factor analyses were used to investigate the structure of the climate model.Main findings: The revised 12-factor model (after the confirmatory factor analysis fitted the data best and the researchers therefore decided to proceed with the revised 12-factor model (11 dimensions for further analysis. A stepwise regression was conducted and nine dimensions of organisational climate were found to predict job satisfaction. The results indicated a strong positive correlation (r = 0.813, p< 0.01 between organisational climate and the dependent variable of job satisfaction.Practical implications: This study provided support for the view that line managers and human resource practitioners should be aware that different biographical groups have different needs that can influence their job satisfaction levels and different perceptions of the climate within the organisation and that this impacts on their behaviour.Contribution: The findings of this study indicated a

  12. Organisational Socialisation Facilitates Overseas Assignment

    Directory of Open Access Journals (Sweden)

    Pei-Chen (Chiu-Yi/Joy Lee

    2014-09-01

    Full Text Available Cross-cultural adjustment is the biggest challenge that derails expatriate success. In responding to the importance of Taiwanese expatriates’ adjustment in Mainland China, this study tests, as a critical factor, Socialisation affecting the adjustment of Taiwanese expatriates in Mainland China. As a result, this study critically reviews previous studies on expatriate success, shedding light on key factors in the area of expatriate adjustment and providing multiple guidelines for human resource supervisors and senior executives hoping to ensure expatriate success. The definitions of success and methodological approaches are discussed, and suggestions for improving empirical research in this area are presented. Academic researchers and international enterprises can perhaps clarify whether all international expatriates are affected by the factor as organisational socialisation, and also whether the proposed model can be utilised in studying expatriates in different professional areas from those of this study. Finally, the results and their implications for research and the business world are discussed. This may assist not only Taiwanese MNCs but also global enterprises to perform their international business more efficiently in Mainland China.

  13. Chromosomal abnormalities and autism

    Directory of Open Access Journals (Sweden)

    Farida El-Baz

    2016-01-01

    Conclusion: Chromosomal abnormalities were not detected in the studied autistic children, and so the relation between the genetics and autism still needs further work up with different study methods and techniques.

  14. Chromosome condensation and segmentation

    International Nuclear Information System (INIS)

    Some aspects of chromosome condensation in mammalians -humans especially- were studied by means of cytogenetic techniques of chromosome banding. Two further approaches were adopted: a study of normal condensation as early as prophase, and an analysis of chromosome segmentation induced by physical (temperature and γ-rays) or chemical agents (base analogues, antibiotics, ...) in order to show out the factors liable to affect condensation. Here 'segmentation' means an abnormal chromosome condensation appearing systematically and being reproducible. The study of normal condensation was made possible by the development of a technique based on cell synchronization by thymidine and giving prophasic and prometaphasic cells. Besides, the possibility of inducing R-banding segmentations on these cells by BrdU (5-bromodeoxyuridine) allowed a much finer analysis of karyotypes. Another technique was developed using 5-ACR (5-azacytidine), it allowed to induce a segmentation similar to the one obtained using BrdU and identify heterochromatic areas rich in G-C bases pairs

  15. Chromosomal Abnormalties with Epilepsy

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2005-02-01

    Full Text Available The correlation between specific chromosome abnormalties and various epilepsies was investigated by a study of 76 patients’ records obtained by questionnaires distributed to members of Kyoto Multi-institutional Study Group of Pediatric Neurology.

  16. Structural organization of the inactive X chromosome in the mouse.

    Science.gov (United States)

    Giorgetti, Luca; Lajoie, Bryan R; Carter, Ava C; Attia, Mikael; Zhan, Ye; Xu, Jin; Chen, Chong Jian; Kaplan, Noam; Chang, Howard Y; Heard, Edith; Dekker, Job

    2016-07-28

    X-chromosome inactivation (XCI) involves major reorganization of the X chromosome as it becomes silent and heterochromatic. During female mammalian development, XCI is triggered by upregulation of the non-coding Xist RNA from one of the two X chromosomes. Xist coats the chromosome in cis and induces silencing of almost all genes via its A-repeat region, although some genes (constitutive escapees) avoid silencing in most cell types, and others (facultative escapees) escape XCI only in specific contexts. A role for Xist in organizing the inactive X (Xi) chromosome has been proposed. Recent chromosome conformation capture approaches have revealed global loss of local structure on the Xi chromosome and formation of large mega-domains, separated by a region containing the DXZ4 macrosatellite. However, the molecular architecture of the Xi chromosome, in both the silent and expressed regions,remains unclear. Here we investigate the structure, chromatin accessibility and expression status of the mouse Xi chromosome in highly polymorphic clonal neural progenitors (NPCs) and embryonic stem cells. We demonstrate a crucial role for Xist and the DXZ4-containing boundary in shaping Xi chromosome structure using allele-specific genome-wide chromosome conformation capture (Hi-C) analysis, an assay for transposase-accessible chromatin with high throughput sequencing (ATAC-seq) and RNA sequencing. Deletion of the boundary disrupts mega-domain formation, and induction of Xist RNA initiates formation of the boundary and the loss of DNA accessibility. We also show that in NPCs, the Xi chromosome lacks active/inactive compartments and topologically associating domains (TADs), except around genes that escape XCI. Escapee gene clusters display TAD-like structures and retain DNA accessibility at promoter-proximal and CTCF-binding sites. Furthermore, altered patterns of facultative escape genes indifferent neural progenitor clones are associated with the presence of different TAD

  17. Structural organization of the inactive X chromosome in the mouse.

    Science.gov (United States)

    Giorgetti, Luca; Lajoie, Bryan R; Carter, Ava C; Attia, Mikael; Zhan, Ye; Xu, Jin; Chen, Chong Jian; Kaplan, Noam; Chang, Howard Y; Heard, Edith; Dekker, Job

    2016-07-28

    X-chromosome inactivation (XCI) involves major reorganization of the X chromosome as it becomes silent and heterochromatic. During female mammalian development, XCI is triggered by upregulation of the non-coding Xist RNA from one of the two X chromosomes. Xist coats the chromosome in cis and induces silencing of almost all genes via its A-repeat region, although some genes (constitutive escapees) avoid silencing in most cell types, and others (facultative escapees) escape XCI only in specific contexts. A role for Xist in organizing the inactive X (Xi) chromosome has been proposed. Recent chromosome conformation capture approaches have revealed global loss of local structure on the Xi chromosome and formation of large mega-domains, separated by a region containing the DXZ4 macrosatellite. However, the molecular architecture of the Xi chromosome, in both the silent and expressed regions,remains unclear. Here we investigate the structure, chromatin accessibility and expression status of the mouse Xi chromosome in highly polymorphic clonal neural progenitors (NPCs) and embryonic stem cells. We demonstrate a crucial role for Xist and the DXZ4-containing boundary in shaping Xi chromosome structure using allele-specific genome-wide chromosome conformation capture (Hi-C) analysis, an assay for transposase-accessible chromatin with high throughput sequencing (ATAC-seq) and RNA sequencing. Deletion of the boundary disrupts mega-domain formation, and induction of Xist RNA initiates formation of the boundary and the loss of DNA accessibility. We also show that in NPCs, the Xi chromosome lacks active/inactive compartments and topologically associating domains (TADs), except around genes that escape XCI. Escapee gene clusters display TAD-like structures and retain DNA accessibility at promoter-proximal and CTCF-binding sites. Furthermore, altered patterns of facultative escape genes indifferent neural progenitor clones are associated with the presence of different TAD

  18. Chimpanzee chromosome 13 is homologous to human chromosome 2p

    Energy Technology Data Exchange (ETDEWEB)

    Sun, N. C.; Sun, C. R.Y.; Ho, T.

    1977-01-01

    Similarities between human and chimpanzee chromosomes are shown by chromosome banding techniques and somatic cell hybridization techniques. Cell hybrids were obtained from the chimpanzee lymphocyte LE-7, and the Chinese hamster mutant cell, Gal-2. Experiments showed that the ACPL, MDHs, and Gal-Act genes could be assigned to chimpanzee chromosome 13, and since these genes have been assigned to human chromosme 2p, it is suggested that chimpanzee chromosome 13 is homologous to human chromosome 2p. (HLW)

  19. Dosage Effects of X and Y Chromosomes on Language and Social Functioning in Children with Supernumerary Sex Chromosome Aneuploidies: Implications for Idiopathic Language Impairment and Autism Spectrum Disorders

    Science.gov (United States)

    Lee, Nancy Raitano; Wallace, Gregory L.; Adeyemi, Elizabeth I.; Lopez, Katherine C.; Blumenthal, Jonathan D.; Clasen, Liv S.; Giedd, Jay N.

    2012-01-01

    Background: Supernumerary sex chromosome aneuploidies (X/Y-aneuploidies), the presence of extra X and/or Y chromosomes, are associated with heightened rates of language impairments and social difficulties. However, no single study has examined different language domains and social functioning in the same sample of children with tri-, tetra-, and…

  20. Chromosome doubling method

    Science.gov (United States)

    Kato, Akio

    2006-11-14

    The invention provides methods for chromosome doubling in plants. The technique overcomes the low yields of doubled progeny associated with the use of prior techniques for doubling chromosomes in plants such as grasses. The technique can be used in large scale applications and has been demonstrated to be highly effective in maize. Following treatment in accordance with the invention, plants remain amenable to self fertilization, thereby allowing the efficient isolation of doubled progeny plants.

  1. Approaches to Maintaining and Building Organisational Knowledge

    International Nuclear Information System (INIS)

    Conclusions: • Involvement of people is one of the most important enablers of successful KM; • KM focuses on organisational knowledge that is needed for achieving business goals; • Working culture and KM activities embedded in the ways of working are essential for management of organisational knowledge; • Formal KM approach is needed as well, and one of its objectives is to support informal KM activities; • For a successful management of organisational knowledge, KM related functions need to be identified and understood as one entity

  2. Organisational IT managed from the shop floor

    DEFF Research Database (Denmark)

    Bolmsten, Johan; Dittrich, Yvonne

    2015-01-01

    Modern organisations need to be able to adjust to changes in the environment, changes which are ever more rapid, and in doing so capitalise on the creativity and innovations of their employees. As suggested by Boulus-Rødje and Bjørn (Chap. 14), information technology (IT) applications today...... are likely to take the form of complex, integrated infrastructures, supporting collaboration within and across organisations. This places requirements on the IT infrastructure. As the work practices within an organisation change, the supporting infrastructure also needs to evolve....

  3. The Dynamics of Organisation and Work

    DEFF Research Database (Denmark)

    Nielsen, Peter

    driver for this general trend is globalisation of the economic relations and growth in technological enhancements. The conditions of globalisation and the technological developments directly or indirectly put high pressure on most organisations in both private and public sector. The high pressure...... international comparative data on organisational changes and economic as well as social performance of the emerging new work organisations. The Meadow project has created instruments to produce such international comparative data and the aim of this paper is to present the measurement framework of Meadow...

  4. Corporate information systems in health organisations.

    Science.gov (United States)

    Smith, J

    1997-01-01

    This paper presents an overview of the nature of corporate information systems and their applications in health organisations. It emphasises the importance of financial and human resource information in the creation of a corporate data model. The paper summarises the main features of finance and human resource systems as they are used in health organisations. It looks at a series of case studies carried out in health organisations, which were selected on the basis of their representation of different aspects of service delivery. It also discusses the theoretical and practical perspectives of the systems themselves, their roles in information management, executive and decision support, and in planning and forecasting. PMID:10173702

  5. Micromechanics of human mitotic chromosomes

    International Nuclear Information System (INIS)

    Eukaryote cells dramatically reorganize their long chromosomal DNAs to facilitate their physical segregation during mitosis. The internal organization of folded mitotic chromosomes remains a basic mystery of cell biology; its understanding would likely shed light on how chromosomes are separated from one another as well as into chromosome structure between cell divisions. We report biophysical experiments on single mitotic chromosomes from human cells, where we combine micromanipulation, nano-Newton-scale force measurement and biochemical treatments to study chromosome connectivity and topology. Results are in accord with previous experiments on amphibian chromosomes and support the 'chromatin network' model of mitotic chromosome structure. Prospects for studies of chromosome-organizing proteins using siRNA expression knockdowns, as well as for differential studies of chromosomes with and without mutations associated with genetic diseases, are also discussed

  6. Communicating for organisational change: exploring links between Internet organisational communications and organisational change in a quality context

    OpenAIRE

    McGuckian, Rosheen

    2000-01-01

    Internal communications is identified as being a key lever of organisational change. The literature consistently puts forward a comprehensive list of guidelines for communicating for change, but these guidelines tend to be prescribed universally and with no explanation given as to why a relationship between the two might exist Research was earned out to explore this relationship, using total quality as the context of organisational change. Through the findings of a questionnaire completed...

  7. Studies in Organisational Semiotics: an introduction

    Directory of Open Access Journals (Sweden)

    Rodney Clarke

    2001-05-01

    Full Text Available The broad application of semiotic approaches to organisations has been considered by a number of information systems academics to be a necessary advance in information systems theory (see for examples Land 1985, Rzevski 1985, and Tully 1985. Along with psychology and sociology, semiotics is considered to be a foundation discipline for information systems within the IFIP WG 8.1 FRISCO Framework (Falkenberg, et al eds/ 2000. Semiotics examines the processes of production and consumption of meanings in organisations, institutions and society, and their underlying mechanisms by means of what Pap (1991, 47 refers to as a "...systematic analysis of patterns of interpretive behaviour". Although often unacknowledged, meaning is central to any definition of an information system. While the concept of meaning and meaning making is difficult to define, semiotic theory can assist by emphasising the distinctions between 'information', 'meaning', 'sense' and 'reference' for example (see Noth 1990, 92-102. Eco (1976, 8, provides a broad definition of semiotics as the study of "...all cultural processes as processes of communication". Cultural processes are interpreted to include organisational contexts and processes thereby providing a link between systems and organisations. Most applied semiotic studies start by identifying or defining one or more models of the sign as the basic unit of analysis. Signs are usually glossed as 'something that stands for something else in some capacity or another'. Depending on the model of the sign, mention may be made to an entity for whom the 'stands for' relationship applies. For a discipline often defined as the 'study of signs', there are a plethora of distinct sign models from which to choose. The reader is directed to Winfried Noth's Handbook of Semiotics for a detailed description of different sign typologies, sign models and disciplinary history (Noth 1990, 79-91. The period of modern semiotics starts at the beginning

  8. CERN’s new organisational structure

    CERN Document Server

    2009-01-01

    CERN’s new organisational structure was made public on 1st December 2008. All the changes are explained in detail on a new web portal. As you learned on 1st December last year, CERN’s new organisational structure took effect on 1st January, under the leadership of new Director-General, Rolf Heuer. To explain the new structure, the Human Resources (HR) Department set up a Web portal in December. This portal (NICE user name and password required) displays the new organisation chart and explains the differences between the old and new structures. A "Frequently Asked Questions" page provides additional information. If you don’t find the answer to your specific question, a discussion forum is also available. You can obtain the contact details of your Human Resources Advisor by clicking on a link that takes you to a dedicated web page. Finally, all documents pertaining to the new organisational structure, including powerpoint pres...

  9. Privacy Management and Accountability in Global Organisations

    OpenAIRE

    Pearson, Siani

    2014-01-01

    Organisations that operate in a global environment can be subject to potentially diverse and complex regulatory requirements. This paper explains some of the key issues that corporate governance faces related to privacy and some mechanisms for addressing these.

  10. Economic Agents, Ethics and International Economic Organisations

    Directory of Open Access Journals (Sweden)

    Bruno S. Sergi

    2006-03-01

    Full Text Available The last sixty years have seen international economic organisations maintain a position at the forefront of promoting economic growth and socioeconomic development. These organisations have not achieved as much against certain benchmarks, and several observers have accused them of being unqualified to meet the needs of the poor, and even found them guilty of something worse, such as famine and disease. Although these organisations have organisational behaviour and skills, many of their funded projects lack forcefulness to such an extent that they should only be answerable through achieving certain economic facts by way of ethics and morals. We design ethics and morals as two interconnected concepts, and the rationale that binds all economic agents to their respective obligations must be interpreted by effective courses of action dictated by economic realities.

  11. Internal corporate venturing during organisational change

    DEFF Research Database (Denmark)

    Kjærgaard, Annemette L.

    2004-01-01

    that a continuous stream of new ideas and initiatives create new opportunities and ensure that the company stays viable by adapting to new internal and external challenges. This has been pursued in studies of strategy formation (Mintzberg, 1978), strategic change (Pettigrew, 1988) and internal corporate venturing...... (Burgelman, 1983b, 2002) and is still a central issue in the strategic management discourse. It is generally acknowledged that continuous change is important for organisations' survival in a changing world. On the other hand the need for stability and continuity in form of a clear and strong corporate...... the question of how organisational actors' perception of organisational identity influences the strategy-making process during organisational change. The study adopts an evolutionary approach to the unfolding of the strategy-making process, using the variation-selection-retention framework of cultural...

  12. What can management theories offer evidence-based practice? A comparative analysis of measurement tools for organisational context

    Directory of Open Access Journals (Sweden)

    Pennington Lindsay

    2009-05-01

    Full Text Available Abstract Background Given the current emphasis on networks as vehicles for innovation and change in health service delivery, the ability to conceptualise and measure organisational enablers for the social construction of knowledge merits attention. This study aimed to develop a composite tool to measure the organisational context for evidence-based practice (EBP in healthcare. Methods A structured search of the major healthcare and management databases for measurement tools from four domains: research utilisation (RU, research activity (RA, knowledge management (KM, and organisational learning (OL. Included studies were reports of the development or use of measurement tools that included organisational factors. Tools were appraised for face and content validity, plus development and testing methods. Measurement tool items were extracted, merged across the four domains, and categorised within a constructed framework describing the absorptive and receptive capacities of organisations. Results Thirty measurement tools were identified and appraised. Eighteen tools from the four domains were selected for item extraction and analysis. The constructed framework consists of seven categories relating to three core organisational attributes of vision, leadership, and a learning culture, and four stages of knowledge need, acquisition of new knowledge, knowledge sharing, and knowledge use. Measurement tools from RA or RU domains had more items relating to the categories of leadership, and acquisition of new knowledge; while tools from KM or learning organisation domains had more items relating to vision, learning culture, knowledge need, and knowledge sharing. There was equal emphasis on knowledge use in the different domains. Conclusion If the translation of evidence into knowledge is viewed as socially mediated, tools to measure the organisational context of EBP in healthcare could be enhanced by consideration of related concepts from the organisational

  13. A Multiple Source Approach to Organisational Justice: The Role of the Organisation, Supervisors, Coworkers, and Customers

    Directory of Open Access Journals (Sweden)

    Agustin Molina

    2015-07-01

    Full Text Available The vast research on organisational justice has focused on the organisation and the supervisor. This study aims to further this line of research by integrating two trends within organisational justice research: the overall approach to justice perceptions and the multifoci perspective of justice judgments. Specifically, this study aims to explore the effects of two additional sources of justice, coworker-focused justice and customer-focused justice, on relevant employees’ outcomes—burnout, turnover intentions, job satisfaction, and workplace deviance— while controlling the effect of organisation-focused justice and supervisor-focused justice. Given the increased importance attributed to coworkers and customers, we expect coworker-focused justice and customer-focused justice to explain incremental variance in the measured outcomes, above and beyond the effects of organisation-focused justice and supervisor-focused justice. Participants will be university students from Austria and Germany employed by service organisations. Data analysis will be conducted using structural equation modeling.

  14. Intra-organisational accounting during negotiation processes for inter-organisational control

    DEFF Research Database (Denmark)

    Jakobsen, Morten

    relationships. It addresses the question of how intra-organisational management accounting practices affect the ability to conduct inter-organisational relationships. A qualitative case study is used to gather information from an electronics company. The company enters its inter-organisational relationships....... The study concludes that an important role of management accounting is to reveal the intra-organisational cost consequences of proposals made by suppliers during negotiation processes. Thereby cost information becomes an integrated part of the counter-proposals generated and actively used during......To date the literature on management and management accounting within inter-organisational relationships has mainly focussed on managing the interface between the supplier and the buyer. In contrast to most previous research, this study examines the internal practices of a company engaged in inter-organisational...

  15. Organisational and strategic communication research: european perspectives

    OpenAIRE

    Gonçalves, Gisela Marques Pereira; Somerville, Ian; Melo, Ana

    2013-01-01

    Série: Pesquisas em comunicação (Excerto) Created in 2006, the Organisational and Strategic Communication Section of the European Communication Research and Education Association (ECREA) aims to promote an active and critical dialogue among scholars involved in the study of Organisational and Strategic Communication (OSC) and to propose new research topics and debate concepts relevant to the interdisciplinary growth of this field of studies. At the 4th international ECREA Co...

  16. The Organisational Gap Model for Hotel Management

    OpenAIRE

    Maja Uran

    2010-01-01

    This paper describes the development of the organisational gap model for hotel management. It descries a management measurement instrument that helps to assess the 3 organizational service gaps that are preconditions for delivering service quality (the positioning gap, specification gap and evaluation gap). The described theoretical model was constructed based upon the four organisational gaps of the Parasuraman et al. service quality model, then redefined and reassessed. Data were gathered o...

  17. Is the Lean Organisation a Complex System?

    OpenAIRE

    Masai, Pierre; Parrend, Pierre; Toussaint, Nicolas; Collet, Pierre

    2015-01-01

    International audience; 3 Toyota Motor Europe, Bruxelles, Belgium 4 Complex System Digital Campus (UNESCO Unitwin) http://unitwin-cs.org/ Abstract The Lean Organisation enjoys a tremendous success. It was first developed within Toyota Motor Corporation in the Automotive Industry, but was then adopted by many organisations in all fields of human activities. A deeper observation of this success displays behaviours of complex systems: a high number of agents interact ...

  18. Labour organisation on robotic milking dairy farms.

    OpenAIRE

    Sonck, B.R.

    1996-01-01

    1. Research issuesThe research described in this dissertation is focused on the effects of the integration of the milking robot in a dairy farm on the labour organisation at operational and tactical level. Attention was paid to the future requirements concerning human labour and labour (re)organisation with respect to the complex interaction between the cows and an automatic milking system (AMS) on a robotic milking dairy farm. The study was divided in a number of research issues (Chapter 1) ...

  19. Key variables of organisation design in servitization

    OpenAIRE

    Kreye, Melanie; Jensen, Per Langaa

    2014-01-01

    Manufacturing companies offering additional service with their products need to change their organisation design to accommodate for the service business. Using the star model, this paper investigates organisation design in terms of strategy, structure, processes, rewards and people as a response to external environment to ensure performance. We present a case in the European renewable energy sector. The findings show three key variables: (i) processes need to enable information flow between b...

  20. The relationship between organisational communication and perception.

    Science.gov (United States)

    Marynissen, H M F

    2011-01-01

    Both researchers and managers search for the most appropriate form of organisational communication. The aim of such an organisational communication is to influence the receivers' perception to confirm, adapt or change behaviour according to the sender's intention. This paper argues that to influence the receivers' perception, a specific form of communication that is embedded in a specific organisational culture is required. It also demands prior knowledge of the existing organisational schemata and the current perception concerning the topic that has to be communicated. The rationale is that three obstacles hinder the objectives of traditional communication strategies to influence perception according to the sender's objectives. The first challenge is that a receiver of a certain message never garners one single, clearly pronounced message conveyed by one single person. Yet, few studies are based on multiple messages from various sources. This makes most of the communication strategies in use obsolete. The second strain is the dual mode of thinking that forms organisational members' perceptions: the heuristic and the cogitative (Taleb, 2010). Most organisational communication theories are based on the paradigm in which receivers of information process this information in a rational way, while research in the field of neurobiology (Lehrer, 2009) indicates that rationality is dominated by emotions. The third difficulty is that organisational members constrain to well-established, ingrained schemas (Labianca et al., 2000; Balogun and Johnson, 2004). Based on these existing schemas, the scattered information from multiple sources, and the inability to process that information through cognitive reasoning, organisational members construct perceptions that are not in line with the objectives of the sender's communication. This article reviews different communication theories, points out key concepts in the literature on individual and collective perceptions, and suggests

  1. Organisational learning is crystallised into artefacts

    DEFF Research Database (Denmark)

    Bertelsen, Olav Wedege

    1996-01-01

    In this note I understand organisational learning in terms of manifest crystallisation of collective experience into artefacts, and I attempt to integrate an engineering and an emancipatory perspective. A Danish music festival serves as an example.......In this note I understand organisational learning in terms of manifest crystallisation of collective experience into artefacts, and I attempt to integrate an engineering and an emancipatory perspective. A Danish music festival serves as an example....

  2. Organisational Structure – Structures for Growth

    OpenAIRE

    Ramskill, Jason

    2011-01-01

    Summary Rexam Plc has articulated that part of the company’s strategy is to make a carefully managed expansion into the emerging markets. In this report I suggest an alternative structure for Rexam to enable implementation of such a strategy and propose a business process for designing effective organisational structures. The work of Chandler (1962) charts the evolution of company organisational structures from the early centralised structures of the 1920s to the multidivisional str...

  3. Using Intelligent Agents to understand organisational behaviour

    CERN Document Server

    Celia, Helen; Robinson, Mark; Siebers, Peer-Olaf; Aickelin, Uwe; Sprigg, Christine

    2008-01-01

    This paper introduces two ongoing research projects which seek to apply computer modelling techniques in order to simulate human behaviour within organisations. Previous research in other disciplines has suggested that complex social behaviours are governed by relatively simple rules which, when identified, can be used to accurately model such processes using computer technology. The broad objective of our research is to develop a similar capability within organisational psychology.

  4. A Model of Project and Organisational Dynamics

    OpenAIRE

    Jenny Leonard

    2012-01-01

    The strategic, transformational nature of many information systems projects is now widely understood. Large-scale implementations of systems are known to require significant management of organisational change in order to be successful. Moreover, projects are rarely executed in isolation – most organisations have a large programme of projects being implemented at any one time. However, project and value management methodologies provide ad hoc definitions of the relationship between a project ...

  5. The Compilation of Math Pattern for Productivity in Educational Organisations, Service Organisations (Municipalities), and Industrial Organisations Based on Organisational Climate in Iran

    OpenAIRE

    Fattah Nazem

    2008-01-01

    The purpose of the present study is to investigate the relationship between organisational climate with the productivity of managers' services in educational, service, and industrial organisations, in order to present a suitable management pattern. The population of the research consist of all managers who work in Tehran high schools, municipalities managers, and managers of the National Company Petro-Chemical Industries. Two hundred and forty five High School managers, 52 municipalities mana...

  6. Antecedents and Outcomes of Organisational Virtues

    OpenAIRE

    Martin-Moreno, Jose Felix

    2010-01-01

    Recent years have seen an increasing interest in the application of virtue ethics in business as opposed to consequentialist or deontogical ethics. However the focus has often been on virtues at the individual as opposed to the organizational level of analysis.It is proposed that the organisational virtues intermediate individual (leadership) and group level constructs (organisational citizenship behaviours, corporate citizenship) to foster intrinsic motivation through a focus on eudemonia (m...

  7. Highly optimised global organisation of metabolic networks

    OpenAIRE

    Tanaka, R.; Csete, M.; Doyle, J.

    2005-01-01

    High-level, mathematically precise descriptions of the global organisation of complex metabolic networks are necessary for understanding the global structure of metabolic networks, the interpretation and integration of large amounts of biologic data (sequences, various -omics) and ultimately for rational design of therapies for disease processes. Metabolic networks are highly organised to execute their function efficiently while tolerating wide variation in their environment. These network...

  8. The relationship between organisational climate and employee satisfaction in a South African information and technology organisation

    OpenAIRE

    Monia L. Castro; Nico Martins

    2010-01-01

    Orientation: Organisational climate and job satisfaction are distinct but related constructs, and both appear to influence employees’ understanding of the work environment and their level of job satisfaction.Research purpose: The objective of this study was to explore the relationship between organisational climate and job satisfaction to determine whether employees’ perceptions of the work environment influence their level of job satisfaction.Motivation for the study: Organisations are facin...

  9. Employee Engagement and Organisational Performance in A Public Sector Organisation in Zimbabwe

    OpenAIRE

    Peter Sibanda; Taruona Muchena; Farai Ncube

    2014-01-01

    The research analyses employee engagement as a precursor to unlocking organisational performance in a public sector organisation in Zimbabwe. The organisation (herein referred to as Zim-PSO), which has been plagued by operational and people challenges, is essentially locked in underperformance, which, it is argued, could improve as employee engagement levels also improved. A sample of 50 subjects participated in the research. Data were gathered using a combination of unstructured interviews, ...

  10. An Analysis of Organisational Culture of Integrated Public Organisations: The case of Thailand

    OpenAIRE

    Phookpan, Pantharak

    2013-01-01

    The theme of the re-aggregation of public organisations has been embraced in the recent public sector reforms of some developed countries such as the UK. The re-aggregation of public organisations may benefit the government in terms of focusing its interests on policy coordination. This is an alternative way of reforming the public sector in order to increase greater outcomes and the performance of public organisations with regard to the achievement of particular policy goals. The reform inev...

  11. Organisational conflict: Reflections on managing conflict, identities and values in a selected South African organisation

    OpenAIRE

    Claude-Hélène Mayer; Lynette Louw

    2009-01-01

    This article presents a case study on organisational conflict in a selected international organisation in the South African automotive industry to increase the contextual understanding of the topic. Data was gathered through in-depth interviews with 45 senior and middle managers in the selected international organisation at the head off ce and two branches in Gauteng and at one branch in KwaZulu-Natal. The data analysis was conducted through content analysis as well as triangulation of dat...

  12. Organisational Social Capital through Corporate Social Performance

    Directory of Open Access Journals (Sweden)

    A.H. Niţu

    2014-06-01

    Full Text Available The purpose of this study is to identify the correlation between the corporate social responsibility performance and the Organisational Social Capital. Inductively, through grounded theory, this paper uses secondary data to develop a theoretical model which presents the relationship between the following concepts: business codes, stakeholders, Corporate Social Performance (CSP and Organisational Social Capital (OSC. This study brings together two main areas of research, namely: Organisational Social Capital and business ethics. This represents a gap in the literature, to which this research will address. Three propositions are put forward and discussed using secondary data collection methods. The findings suggest that there is a strong correlation between the characteristics which improve the quality of organisation-stakeholders relationship and the effectiveness of implementing business codes and, therefore, the increasing Corporate Social Performance. The proposed ethical framework has, at the same time, a similar effect by incrementing Organisational Social Capital, because it shares similar features with the relation between organisation-stakeholders, business codes and CSP.

  13. Economic and Organisational Wisdom for Asian Century

    Directory of Open Access Journals (Sweden)

    Arup BARMAN

    2015-06-01

    Full Text Available The Asian Century is rich and already 14 years old. Today, Asia is in the middle of a historic transformation which has brought both challenges and opportunities. To meet these challenges, Asian leaders need to devise bold and innovative national policies for pursuing avenues for regional and global cooperation. In the similar way organisation and business in Asia will transform. This transformative whirlpool calls the Asian private sector and public sector organisations for absorption of new wisdom, values and principles in place of 20th century’s management. Overall, the capacity to respond to the changing global economic landscape through flexibility and adaptivness will carry a high premium. This paper delve the issues how Asian Organisations have already been used the wisdom during the global chaos. Deriving from the examples of Asia in the midst of global chaos in many points of time, this present paper attempts to re-focus on organisational wisdom of commitment and ability for Asian organisation, modernization, governance and helping to retool institutions, for enhancing transparency, and finally to develop accountability for organisational resilience and survival.

  14. Exploring inertia in a typical state organisation

    Directory of Open Access Journals (Sweden)

    G. J. Louw

    2004-10-01

    Full Text Available Those organisations which do not change according to environmental pressures, suffer from organisational inertia. The purpose of this study is to explore the manifestation of organisational inertia in the target organisation. The target population for this study was a group of trainees, representing the geographic and demographic levels of a particular state department. In South Africa, surveys of this nature were only executed in the corporate sector. The results indicate that organisational inertia is a phenomenon that affects both corporate and governmental organisations. Opsomming Organisasies wat nie ooreenkomstig omgewingsdruk verander nie, ly aan organisasietraagheid. Die doel van die studie is om organisasietraagheid te konseptualiseer en die manifestasie daarvan in die teikenorganisasie te ondersoek. Die teikenpopulasie bestaan uit ’n groep kursusgangers wat die demografiese en geografiese samestelling van ‘n tipiese staatsdepartement verteenwoordig. In Suid -Afrika is navorsing van hierdie aard nog net in die korporatiewe sektor uitgevoer. Die resultate toon aan dat organisasietraagheid ‘n faktor is wat beide die korporatiewe omgewing en staatsorganisasies beïnvloed.

  15. [The current and future organisational structure of the OIE (World Organisation for Animal Health)].

    Science.gov (United States)

    Crespo León, F; Ruiz Mercader, J; Sabater Sánchez, R; Rodríguez Ferri, E F; Crespo Azofra, L

    2003-12-01

    The authors analyse the organisational structure of the OIE (World organisation for animal health), highlighting the roles of the Central Bureau, the Specialist Commissions, Regional Commissions, working groups and ad hoc groups, Regional Representations, Reference Laboratories and Collaborating Centres. The paper also includes some suggestions as to how the OIE could work more closely with its 'customers', that is, the Member Countries. These suggestions are based on current theories of organisational flexibility, and take into account not only the current organisational structure of the OIE, but also the Strategic Plan and the Working Plan, which were adopted at the 69th General Session of the OIE International Committee in 2001.

  16. Organisational conflict: Reflections on managing conflict, identities and values in a selected South African organisation

    Directory of Open Access Journals (Sweden)

    Claude-Hélène Mayer

    2009-04-01

    Full Text Available This article presents a case study on organisational conflict in a selected international organisation in the South African automotive industry to increase the contextual understanding of the topic. Data was gathered through in-depth interviews with 45 senior and middle managers in the selected international organisation at the head off ce and two branches in Gauteng and at one branch in KwaZulu-Natal. The data analysis was conducted through content analysis as well as triangulation of data. The research results show that managers in this industry in post-apartheid South Africa experience organisational conflicts that are associated with their managerial values and identities.

  17. Interphase chromosome positioning in in vitro porcine cells and ex vivo porcine tissues

    Directory of Open Access Journals (Sweden)

    Foster Helen A

    2012-11-01

    Full Text Available Abstract Background In interphase nuclei of a wide range of species chromosomes are organised into their own specific locations termed territories. These chromosome territories are non-randomly positioned in nuclei which is believed to be related to a spatial aspect of regulatory control over gene expression. In this study we have adopted the pig as a model in which to study interphase chromosome positioning and follows on from other studies from our group of using pig cells and tissues to study interphase genome re-positioning during differentiation. The pig is an important model organism both economically and as a closely related species to study human disease models. This is why great efforts have been made to accomplish the full genome sequence in the last decade. Results This study has positioned most of the porcine chromosomes in in vitro cultured adult and embryonic fibroblasts, early passage stromal derived mesenchymal stem cells and lymphocytes. The study is further expanded to position four chromosomes in ex vivo tissue derived from pig kidney, lung and brain. Conclusions It was concluded that porcine chromosomes are also non-randomly positioned within interphase nuclei with few major differences in chromosome position in interphase nuclei between different cell and tissue types. There were also no differences between preferred nuclear location of chromosomes in in vitro cultured cells as compared to cells in tissue sections. Using a number of analyses to ascertain by what criteria porcine chromosomes were positioned in interphase nuclei; we found a correlation with DNA content.

  18. Chromosome numbers in Bromeliaceae

    Directory of Open Access Journals (Sweden)

    Cotias-de-Oliveira Ana Lúcia Pires

    2000-01-01

    Full Text Available The present study reports chromosome numbers of 17 species of Bromeliaceae, belonging to the genera Encholirium, Bromelia, Orthophytum, Hohenbergia, Billbergia, Neoglaziovia, Aechmea, Cryptanthus and Ananas. Most species present 2n = 50, however, Bromelia laciniosa, Orthophytum burle-marxii and O. maracasense are polyploids with 2n = 150, 2n = 100 and 2n = 150, respectively, while for Cryptanthus bahianus, 2n = 34 + 1-4B. B chromosomes were observed in Bromelia plumieri and Hohenbergia aff. utriculosa. The chromosome number of all species was determined for the first time, except for Billbergia chlorosticta and Cryptanthus bahianus. Our data supports the hypothesis of a basic number of x = 25 for the Bromeliaceae family and decreasing aneuploidy in the genus Cryptanthus.

  19. Those amazing dinoflagellate chromosomes

    Institute of Scientific and Technical Information of China (English)

    PETER J RIZZO

    2003-01-01

    Dinoflagellates are a very large and diverse group of eukaryotic algae that play a major role in aquatic food webs of both fresh water and marine habitats. Moreover, the toxic members of this group pose a health threat in the form of red tides. Finally, dinoflagellates are of great evolutionary importance,because of their taxonomic position, and their unusual chromosome structure and composition. While the cytoplasm of dinoflagellates is typically eukaryotic, the nucleus is unique when compared to the nucleus of other eukaryotes. More specifically, while the chromosomes of all other eukaryotes contain histones,dinoflagellate chromosomes lack histones completely. There are no known exceptions to this observation: all dinoflagellates lack histones, and all other eukaryotes contain histones. Nevertheless, dinoflagellates remain a relatively unstudied group of eukaryotes.

  20. Chromatin domain boundaries: insulators and beyond

    Institute of Scientific and Technical Information of China (English)

    Gong Hong WEI; De Pei LIU; Chih Chuan LIANG

    2005-01-01

    The eukaryotic genome is organized into functionally and structurally distinct domains, representing regulatory units for gene expression and chromosome behavior. DNA sequences that mark the border between adjacent domains are the insulators or boundary elements, which are required in maintenance of the function of different domains. Some insulators need others enable to play insulation activity. Chromatin domains are defined by distinct sets of post-translationally modified histones. Recent studies show that these histone modifications are also involved in establishment of sharp chromatin boundaries in order to prevent the spreading of distinct domains. Additionally, in some loci, the high-order chromatin structures for long-range looping interactions also have boundary activities, suggesting a correlation between insulators and chromatin loop domains. In this review, we will discuss recent progress in the field of chromatin domain boundaries.

  1. Palliative care. Some organisational considerations.

    Science.gov (United States)

    Welshman, A

    2005-01-01

    consideration. Another important issue faced daily by palliative care physicians is the broad number of chronic conditions which could make use of opioids. Severe cancer pain is the most obvious example of an appropriate use of opioids, but hardly the only one. The North American Chronic Pain Association of Canada (NACPAC) advocates the use of opioids for a wide range of conditions causing severe chronic pain, including lower back pain, inflammatory bowel disease, migraines, AIDS, multiple sclerosis and arthritis. Concerns regarding under treatment of chronic pain have captured the attention of patient advocacy groups, policy makers and scientific organisations. Misconceptions of opioid laws, negative social stigma and lack of valid prescribing alternatives to overcome this, together with paucity of formal provider education confound the issue. Much education needs to be done before opioids will be seen as a safe and reasonable treatment for chronic pain here in Italy.

  2. Domain Engineering - A Software Engineering discipline in Need of Research

    DEFF Research Database (Denmark)

    Bjørner, Dines

    2000-01-01

    , and these again seem more stable than software designs. Thus, almost like the universal laws of physics, it pays off to first develop theories of domains. But domain engineering, as in fact also requirements engineering, really is in need of thoroughly researched development principles, techniques and......Before software can be developed its requirements must be stated. Before requirements can be expressed the application domain must be understood. In this paper we outline some of the basic facets of domain engineering. Domains seem, it is our experience, far more stable than computing requirements...... formal techniques. A brief example of describing stake-holder perspectives will be given - on the background of which we then proceed to survey the notions of domain intrinsics, domain support technologies, domain management & organisation, domain rules & regulations, domain human behaviour, etc. We show...

  3. Action Research and Organisational Learning: A Norwegian Approach to Doing Action Research in Complex Organisations

    Science.gov (United States)

    Eikeland, Olav

    2012-01-01

    The purpose of this article is to present a specific approach to the practice of action research "in complex organisations". Clearly, there are many approaches to the challenge of doing action research in organisations; approaches that are, and also must be, quite context dependent and specific. But my purpose is neither to give an overview nor a…

  4. Responses to Climate Change: Exploring Organisational Learning across Internationally Networked Organisations for Development

    Science.gov (United States)

    Boyd, Emily; Osbahr, Henny

    2010-01-01

    Drawing from the organisational learning and governance literature, this paper assesses four internationally networked governmental and non-governmental organisations in the UK addressing climate change. We analyse how those concerned understand the climate change crisis, what mechanisms are put in place to address information flows, and what…

  5. Strategic Orientation and Organisational Culture in Polish Public Organisations: Insights from the Miles and Snow Typology

    Directory of Open Access Journals (Sweden)

    Wronka-Pośpiech Martyna

    2016-05-01

    Full Text Available Polish public organisations are often perceived as having strong bureaucratic orientation, avoiding both change and risk. However, in the last decade a distinct change in the management model of public organisations can be noticed. Public sector becomes an open ground for mergers and partnerships, entrepreneurial leadership, diversified services and commercialization (Golensky and DeRuiter 1999; Zimmerman and Dart, 1998; Pollitt and Bouckaert, 2004; Walker, 2013]. Public organisations embrace these strategies from the for-profit sector in order to manage change and to be effective. Most importantly, public organisations are adopting these frameworks in order to survive the changing operating environment, including changes in the level of government funding. Our paper draws on the Miles and Snow (1978 typology of generic strategies - prospectors, defenders, analysers, and reactors - to identify different organisational strategies within public organisations providing social services in Poland. In order to assess organisational culture we used the most widespread and used in many empirical studies Cameron and Quinn’s model (2003, the Competing Values Framework (CVF, from which four cultures - adhocracy, clan, market and hierarchy - emerge. The choice of these two providers of social services was dictated by our conviction, that these organisations are critical both for the national economy and for mitigating, counteracting and preventing social exclusion.

  6. Organisational Learning and the Organisational Life Cycle: The Differential Aspects of an Integrated Relationship in SMEs

    Science.gov (United States)

    Tam, Steven; Gray, David E.

    2016-01-01

    Purpose: The purpose of this study is to relate the practice of organisational learning in small- and medium-sized enterprises (SMEs) to the organisational life cycle (OLC), contextualising the differential aspects of an integrated relationship between them. Design/methodology/approach: It is a mixed-method study with two consecutive phases. In…

  7. The Strength of Organisational Culture: Organisational Performance in South African Schools

    Science.gov (United States)

    Bipath, Keshni; Adeyemo, Kolawole Samuel

    2014-01-01

    One of the more frequently cited cultural dimensions within the field of cultural research is that of cultural strength (Cameron & Ettington, 1998). Early published works on organisational culture argue that there is an absolute need for a "strong" culture for organisations to be effective. This study has examined the contradiction…

  8. Inter-organisational response to disasters.

    Science.gov (United States)

    Paturas, James L; Smith, Stewart R; Albanese, Joseph; Waite, Geraldine

    2016-01-01

    Inter-organisational communication failures during times of real-world disasters impede the collaborative response of agencies responsible for ensuring the public's health and safety. In the best of circumstances, communications across jurisdictional boundaries are ineffective. In times of crisis, when communities are grappling with the impact of a disaster, communications become critically important and more complex. Important factors for improving inter-organisational communications are critical thinking and problem-solving skills; inter-organisational relationships; as well as strategic, tactical and operational communications. Improving communication, critical thinking, problem-solving and decision-making requires a review of leadership skills. This discussion begins with an analysis of the existing disaster management research and moves to an examination of the importance of inter-organisational working relationships. Before a successful resolution of a disaster by multiple levels of first responders, the group of organisations must have a foundation of trust, collegiality, flexibility, expertise, openness, relational networking and effective communications. Leaders must also be prepared to improve leadership skills through continual development in each of these foundational areas.

  9. Inter-organisational response to disasters.

    Science.gov (United States)

    Paturas, James L; Smith, Stewart R; Albanese, Joseph; Waite, Geraldine

    2016-01-01

    Inter-organisational communication failures during times of real-world disasters impede the collaborative response of agencies responsible for ensuring the public's health and safety. In the best of circumstances, communications across jurisdictional boundaries are ineffective. In times of crisis, when communities are grappling with the impact of a disaster, communications become critically important and more complex. Important factors for improving inter-organisational communications are critical thinking and problem-solving skills; inter-organisational relationships; as well as strategic, tactical and operational communications. Improving communication, critical thinking, problem-solving and decision-making requires a review of leadership skills. This discussion begins with an analysis of the existing disaster management research and moves to an examination of the importance of inter-organisational working relationships. Before a successful resolution of a disaster by multiple levels of first responders, the group of organisations must have a foundation of trust, collegiality, flexibility, expertise, openness, relational networking and effective communications. Leaders must also be prepared to improve leadership skills through continual development in each of these foundational areas. PMID:27318289

  10. Organisational Communication, The Panacea For Improved Labour Relations

    OpenAIRE

    Dupe Adesubomi Abolade

    2015-01-01

    This paper examined the importance of organisational communication to overall effective labour relations. It looked at organisational communication and its role in labour relations between the parties involved in Labour relations for better business procedures and relationships. It explained the importance of organisational communication in enhancing better labour relations within the organisation and with people outside the organisation. It enumerated the various groups that are involved in ...

  11. The mediating effect of knowledge sharing between organisational culture and turnover intentions of professional nurses

    Directory of Open Access Journals (Sweden)

    Everd J. Jacobs

    2011-03-01

    Full Text Available Professional nurses routinely use highly developed domain knowledge in combination with experiential knowledge to deliver quality care. However, this knowledge is often lost to employers as the migration of professional nurses from the developing countries to the developed world has become a global problem. The objective of this study therefore was to determine the relationships between organisational culture, knowledge sharing and turnover intentions and thereafter to propose knowledge sharing as a mediating variable in this relationship in order to suggest a retention strategy. A cross-sectional field survey design with questionnaires was used on a sample of professional nurses (N = 530 in private and provincial hospitals in South Africa. The tri-variate procedure of Baron and Kenny for mediation testing was adopted. The results indicated that a positive correlation exists between organisational culture and knowledge sharing, but a significant negative correlation between organisational culture and turnover intentions, as well as between knowledge sharing and turnover intentions. Finally, the results indicated that knowledge sharing mediates the relationship between organisational culture and turnover intentions, although with a small effect size.The findings suggest that turnover intentions of nurses can be actively managed through contextual variables such as organisational culture and opportunities for knowledge sharing.

  12. The Y Chromosome

    Science.gov (United States)

    Offner, Susan

    2010-01-01

    The Y chromosome is of great interest to students and can be used to teach about many important biological concepts in addition to sex determination. This paper discusses mutation, recombination, mammalian sex determination, sex determination in general, and the evolution of sex determination in mammals. It includes a student activity that…

  13. Chromosomes, cancer and radiosensitivity

    International Nuclear Information System (INIS)

    Some specific chromosomal abnormalities are associated with certain cancers. The earliest description of such a specific association is the one of the Philadelphia chromosome and myelogenous leukemia (1960). Other congenital karyotype abnormalities are associated with specific cancers. Examples of these are Down's syndrome with leukemia and Klinefelter's syndrome with male breast cancer. Genetic diseases of increased chromosome breakage, or of defective chromosome repair, are associated with greatly increased cancer incidence. Three such diseases have been recognized: 1) Fanconi's anemia, associated with leukemias and lymphomas, 2) Bloom's syndrome, associated with acute leukemias and lymphosarcoma, and 3) ataxia telangiectasia, associated with Hodgkin's disease, leukemia, and lymphosarcomas. Ten percent of individuals with ataxia telangiectasia will develop one of these neoplasms. Individuals with certain of these syndromes display an unusually high radiosensitivity. Radiation therapy for cancers has been fatal in patients who received as low as 3000 rad. This remarkable radiosensitivity has been quantitated in cell cultures from such cases. Evidence suggests that the apparent sensitivity may reflect subnormal ability to repair radiation damage. The rapid proliferation of information in this field stems from the interdigitation of many disciplines and specialties, including cytogenetics, cell biology, molecular biology, epidemiology, radiobiology, and several others. This paper is intended for clinicians; it presents a structured analytic scheme for correlating and classifying this multidisciplinary information as it becomes available

  14. Chromosomes, cancer and radiosensitivity

    Energy Technology Data Exchange (ETDEWEB)

    Samouhos, E.

    1983-08-01

    Some specific chromosomal abnormalities are associated with certain cancers. The earliest description of such a specific association is the one of the Philadelphia chromosome and myelogenous leukemia (1960). Other congenital karyotype abnormalities are associated with specific cancers. Examples of these are Down's syndrome with leukemia and Klinefelter's syndrome with male breast cancer. Genetic diseases of increased chromosome breakage, or of defective chromosome repair, are associated with greatly increased cancer incidence. Three such diseases have been recognized: 1) Fanconi's anemia, associated with leukemias and lymphomas, 2) Bloom's syndrome, associated with acute leukemias and lymphosarcoma, and 3) ataxia telangiectasia, associated with Hodgkin's disease, leukemia, and lymphosarcomas. Ten percent of individuals with ataxia telangiectasia will develop one of these neoplasms. Individuals with certain of these syndromes display an unusually high radiosensitivity. Radiation therapy for cancers has been fatal in patients who received as low as 3000 rad. This remarkable radiosensitivity has been quantitated in cell cultures from such cases. Evidence suggests that the apparent sensitivity may reflect subnormal ability to repair radiation damage. The rapid proliferation of information in this field stems from the interdigitation of many disciplines and specialties, including cytogenetics, cell biology, molecular biology, epidemiology, radiobiology, and several others. This paper is intended for clinicians; it presents a structured analytic scheme for correlating and classifying this multidisciplinary information as it becomes available.

  15. Chromosome surveys of human populations: between epidemiology and anthropology.

    Science.gov (United States)

    de Chadarevian, Soraya

    2014-09-01

    It is commonly held that after 1945 human genetics turned medical and focussed on the individual rather than on the study of human populations that had become discredited. However, a closer look at the research practices at the time quickly reveals that human population studies, using old and new tools, prospered in this period. The essay focuses on the rise of chromosome analysis as a new tool for the study of human populations. It reviews a broad array of population studies ranging from newborn screening programmes to studies of isolated or 'primitive' people. Throughout, it highlights the continuing role of concerns and opportunities raised by the propagation of atomic energy for civilian and military uses, the collection of large data bases and computers, and the role of international organisations like the World Health Organisation and the International Biological Programme in shaping research agendas and carving out a space for human heredity in the postwar era.

  16. Indico central - events organisation, ergonomics and collaboration tools integration

    Science.gov (United States)

    Benito Gonzélez López, José; Ferreira, José Pedro; Baron, Thomas

    2010-04-01

    While the remote collaboration services at CERN slowly aggregate around the Indico event management software, its new version which is the result of a careful maturation process includes improvements which will set a new reference in its domain. The presentation will focus on the description of the new features of the tool, the user feedback process which resulted in a new record of usability. We will also describe the interactions with the worldwide community of users and server administrators and the impact this has had on our development process, as well as the tools set in place to streamline the work between the different collaborating sites. A last part will be dedicated to the use of Indico as a central hub for operating other local services around the event organisation (registration epayment, audiovisual recording, webcast, room booking, and videoconference support)

  17. Indico Central - Events Organisation, Ergonomics and Collaboration Tools Integration

    CERN Document Server

    Gonzalez Lopez, J B; Baron, T; CERN. Geneva. IT Department

    2010-01-01

    While the remote collaboration services at CERN slowly aggregate around the Indico event management software, its new version which is the result of a careful maturation process includes improvements which will set a new reference in its domain. The presentation will focus on the description of the new features of the tool, the user feedback process which resulted in a new record of usability. We will also describe the interactions with the worldwide community of users and server administrators and the impact this has had on our development process, as well as the tools set in place to streamline the work between the different collaborating sites. A last part will be dedicated to the use of Indico as a central hub for operating other local services around the event organisation (registration epayment, audiovisual recording, webcast, room booking, and videoconference support)

  18. Disruption of human vigilin impairs chromosome condensation and segregation.

    Science.gov (United States)

    Wei, Ling; Xie, Xiaoyan; Li, Junhong; Li, Ran; Shen, Wenyan; Duan, Shuwang; Zhao, Rongce; Yang, Wenli; Liu, Qiuying; Fu, Qiang; Qin, Yang

    2015-11-01

    Appropriate packaging and condensation are critical for eukaryotic chromatin's accommodation and separation during cell division. Human vigilin, a multi-KH-domain nucleic acid-binding protein, is associated with alpha satellites of centromeres. DDP1, a vigilin's homolog, is implicated with chromatin condensation and segregation. The expression of vigilin was previously reported to elevate in highly proliferating tissues and increased in a subset of hepatocellular carcinoma patients. Other studies showed that vigilin interacts with CTCF, contributes to regulation of imprinted genes Igf2/H19, and colocalizes with HP1α on heterochromatic satellite 2 and β-satellite repeats. These studies indicate that human vigilin might be involved in chromatin remodeling and regular cell growth. To investigate the potential role of human vigilin in cell cycle, the correlations between vigilin and chromosomal condensation and segregation were studied. Depletion of human vigilin by RNA interference in HepG2 cells resulted in chromosome undercondensation and various chromosomal defects during mitotic phase, including chromosome misalignments, lagging chromosomes, and chromosome bridges. Aberrant polyploid nucleus in telophase was also observed. Unlike the abnormal staining pattern of chromosomes, the shape of spindle was normal. Furthermore, the chromatin showed a greater sensitivity to MNase digestion. Collectively, our findings show that human vigilin apparently participates in chromatin condensation and segregation. PMID:26032007

  19. Telomere dysfunction and chromosome instability

    Energy Technology Data Exchange (ETDEWEB)

    Murnane, John P., E-mail: jmurnane@radonc.ucsf.edu [Department of Radiation Oncology, University of California San Francisco, 2340 Sutter Street, San Francisco, CA 94143-1331 (United States)

    2012-02-01

    The ends of chromosomes are composed of a short repeat sequence and associated proteins that together form a cap, called a telomere, that keeps the ends from appearing as double-strand breaks (DSBs) and prevents chromosome fusion. The loss of telomeric repeat sequences or deficiencies in telomeric proteins can result in chromosome fusion and lead to chromosome instability. The similarity between chromosome rearrangements resulting from telomere loss and those found in cancer cells implicates telomere loss as an important mechanism for the chromosome instability contributing to human cancer. Telomere loss in cancer cells can occur through gradual shortening due to insufficient telomerase, the protein that maintains telomeres. However, cancer cells often have a high rate of spontaneous telomere loss despite the expression of telomerase, which has been proposed to result from a combination of oncogene-mediated replication stress and a deficiency in DSB repair in telomeric regions. Chromosome fusion in mammalian cells primarily involves nonhomologous end joining (NHEJ), which is the major form of DSB repair. Chromosome fusion initiates chromosome instability involving breakage-fusion-bridge (B/F/B) cycles, in which dicentric chromosomes form bridges and break as the cell attempts to divide, repeating the process in subsequent cell cycles. Fusion between sister chromatids results in large inverted repeats on the end of the chromosome, which amplify further following additional B/F/B cycles. B/F/B cycles continue until the chromosome acquires a new telomere, most often by translocation of the end of another chromosome. The instability is not confined to a chromosome that loses its telomere, because the instability is transferred to the chromosome donating a translocation. Moreover, the amplified regions are unstable and form extrachromosomal DNA that can reintegrate at new locations. Knowledge concerning the factors promoting telomere loss and its consequences is

  20. European Science Research Organisations forge closer ties

    CERN Multimedia

    2001-01-01

    Effective collaboration has always been one of the foundations of CERN's success. An essential ingredient for collaboration is communication and a new body EIROFORUM has just held its first meeting in Brussels with the aim of developing greater cross fertilization of ideas and projects. Seven organisations, CERN, EMBL (molecular biology), ESA (space activities), ESO (astronomy and astrophysics), ESRF (synchrotron radiation), ILL (neutron source) and EFDA (fusion) are currently members of EIROFORUM. Common interest between the organisations in computational grid development, materials science, instrumentation, public outreach and technology transfer has made EIROFRUM an essential group for maximising these European research organisations' resources. Increasing involvement in the Sixth Framework Programme, Europe's research guidelines for the next four years, is also one of the goals of the group. CERN takes over the chair of EIROFORUM in July 2001 and the next meeting will be held on site on 24 October .

  1. Preparedness organisations at Nordic nuclear power plants

    International Nuclear Information System (INIS)

    The report presents an overview of Emergency Preparedness Organisations (EPO) in Sweden, Finland and Norway and presentations of insights from a study of the staff positions' work instructions in the command centre in an emergency situation. The results indicate potential for improvement in several areas. A number of the improvements are related to introduction of new technology and they should be seen in connection with ensuring safe and reliable communication lines and power supply. Analysis of the data identified four main categories where further studies could contribute to improvement: 1) Communication and exchange of information. 2) Tools and technology. 3) Staffing and organisation. 4) Procedures. The usefulness of the Man Technology and Organisation method in analysing the emergency management decision-making process within the authorities was considered as an interesting issue for continuation of the project. The interface between utility and authorities was pointed out as an important area for continuation. (Author)

  2. Self-organising structures of lecithin

    Science.gov (United States)

    Shchipunov, Yurii A.

    1997-04-01

    Modern concepts of the self-assembly of amphiphiles are considered on the example of self-organising structures of the natural lecithin. Binary, ternary and multicomponent systems are discussed. A considerable part of the review is devoted to the peculiarities of self-organisation of this phospholipid in non-aqueous media and to the role of polar inorganic solvents. Virtually all of the structures formed by lecithin are examined: micelles, swollen micelles, microemulsions, emulsions, organogels, vesicles (liposomes), and lyotropic liquid crystals. In each specific case, attention is drawn to the dependence of self-assembly at the macroscopic level on interactions at the molecular level, shape of molecules, and their solvation and packing at the interface. The self-organising lecithin structures formed in the interfacial area of immiscible liquids in the course of unrestricted adsorption from the bulk of non-aqueous solution are considered. The bibliography includes 282 references.

  3. Information in organisations: directions for information management

    Directory of Open Access Journals (Sweden)

    Joyce Kirk

    1999-01-01

    Full Text Available The work of managers in small and medium-sized enterprises is very information-intensive and the environment in which it is done is very information rich. But are managers able to exploit the wealth of information which surrounds them? And how can information be managed in organisations so that its potential for improving business performance and enhancing the competitiveness of these enterprises can be realised? Answers to these questions lie in clarifying the context of the practice of information management by exploring aspects of organisations and managerial work and in exploring the nature of information at the level of the organisation and the individual manager. From these answers it is possible to suggest some guidelines for managing the integration of business strategy and information, the adoption of a broadly-based definition of information and the development of information capabilities.

  4. The Organisation of Innovative Activities Across Industries

    DEFF Research Database (Denmark)

    Drejer, Ina; Leiponen, Aija

    2003-01-01

    This paper presents an analysis of the organisation of innovation across sectors in Denmark and Finland. First, patterns of innovative activity within manufacturing and services are identified though applying factor analysis on firm-level innovation survey data from the two countries. The analysis......, the relation between innovation modes and innovation outcome are investigated through regression analysis. The analysis confirms that the way innovation is organised matters for outcome, in terms of type of innovation, as well as in terms of share of sales stemming from innovation. Finally, we explore whether...... innovation modes are aligned with industry classifications, as it is assumed in the original work on technological regimes. Contrary to these assumptions we find that industries by no means are uniform in terms of organisation of innovation; in most cases two or three regimes are represented in a single...

  5. Organisational sensemaking, strategy, structuring and routines

    DEFF Research Database (Denmark)

    Nielsen, Renate

    2001-01-01

    Research objective: The purpose of my research is to generate new understanding of what organisational interpretation, sensemaking and structuring processes in organisations mean in a way that can be used not only retrospectively but actively in strategic planning. In the study of these processes...... my focus will, more specifically, be on how sensemaking processes are transformed into strategy and policies to be applied in daily routines, especially sales/marketing strategy routines. By acquiring a broader comprehension of the structuring and strategising processes the intent is to improve...... organisations' competitiveness. Instead of concentrating on decision making I wish to contribute through a better understanding of the processes leading up to decision making. Knowing what guides decision making could mean an even better possibility of making the right decision and implement it in new...

  6. Rise, fall and resurrection of chromosome territories: a historical perspective. Part I. The rise of chromosome territories

    Directory of Open Access Journals (Sweden)

    T Cremer

    2009-06-01

    Full Text Available It is now generally accepted that chromosomes in the cell nucleus are organized in distinct domains, first called chromosome territories in 1909 by the great cytologist Theodor Boveri. Yet, even today chromosomes have remained enigmatic individuals, whose structures, arrangements and functions in cycling and post-mitotic cells still need to be explored in full detail. Whereas numerous recent reviews describe present evidence for a dynamic architecture of chromosome territories and discuss the potential significance within the functional compartmentalization of the nucleus, a comprehensive historical account of this important concept of nuclear organization was lacking so far. Here, we describe the early rise of chromosome territories within the context of the discovery of chromosomes and their fundamental role in heredity, covering a period from the 1870th to the early 20th century (part I, this volume. In part II (next volume we review the abandonment of the chromosome territory concept during the 1950th to 1980th and the compelling evidence, which led to its resurrection during the 1970th to 1980th.

  7. Microstructure-mobility correlation in self-organised, conjugated polymer field-effect transistors

    DEFF Research Database (Denmark)

    Sirringhaus, H.; Brown, P.J.; Friend, R.H.;

    2000-01-01

    We have investigated the correlation between polymer microstructure and charge carrier mobility in high-mobility, self-organised field-effect transistors of poly-3-hexyl-thiophene (P3HT). Two different preferential orientations of the microcrystalline P3HT domains with respect to the substrate ha...... the charge transport properties of conjugated polymer field-effect devices. (C) 2000 Elsevier Science S.A. All rights reserved.......We have investigated the correlation between polymer microstructure and charge carrier mobility in high-mobility, self-organised field-effect transistors of poly-3-hexyl-thiophene (P3HT). Two different preferential orientations of the microcrystalline P3HT domains with respect to the substrate have...

  8. Chromosomal breakpoints characterization of two supernumerary ring chromosomes 20.

    Science.gov (United States)

    Guediche, N; Brisset, S; Benichou, J-J; Guérin, N; Mabboux, P; Maurin, M-L; Bas, C; Laroudie, M; Picone, O; Goldszmidt, D; Prévot, S; Labrune, P; Tachdjian, G

    2010-02-01

    The occurrence of an additional ring chromosome 20 is a rare chromosome abnormality, and no common phenotype has been yet described. We report on two new patients presenting with a supernumerary ring chromosome 20 both prenatally diagnosed. The first presented with intrauterine growth retardation and some craniofacial dysmorphism, and the second case had a normal phenotype except for obesity. Conventional cytogenetic studies showed for each patient a small supernumerary marker chromosome (SMC). Using fluorescence in situ hybridization, these SMCs corresponded to ring chromosomes 20 including a part of short and long arms of chromosome 20. Detailed molecular cytogenetic characterization showed different breakpoints (20p11.23 and 20q11.23 for Patient 1 and 20p11.21 and 20q11.21 for Patient 2) and sizes of the two ring chromosomes 20 (13.6 Mb for case 1 and 4.8 Mb for case 2). Review of the 13 case reports of an extra r(20) ascertained postnatally (8 cases) and prenatally (5 cases) showed varying degrees of phenotypic abnormalities. We document a detailed molecular cytogenetic chromosomal breakpoints characterization of two cases of supernumerary ring chromosomes 20. These results emphasize the need to characterize precisely chromosomal breakpoints of supernumerary ring chromosomes 20 in order to establish genotype-phenotype correlation. This report may be helpful for prediction of natural history and outcome, particularly in prenatal diagnosis.

  9. Measuring business continuity programmes in large organisations.

    Science.gov (United States)

    Green, Christopher

    2014-01-01

    In the field of business continuity management, organisations commit sums of money (often very large sums) to develop and maintain their continuity capability. Despite this, there is almost no measurement of whether this expense offers value for money, or whether it is targeted in the right areas. This paper will explain some methods of measuring components of a business continuity programme. The important outputs from this measurement activity are to demonstrate that an organisation's continuity capability is improving over time, and to identify areas of weakness that should be targeted during future work. PMID:25193458

  10. Learning Styles and Organisational Development in Practice

    DEFF Research Database (Denmark)

    Brix, Jacob; Lauridsen, Karen M.

    2012-01-01

    Issues of innovation and knowledge management are often treated from an organisational learning perspective. As a complement to this, there is a vast potential in the strategic enhancement of individual learning by implementing learning styles profiles and creating personal learning strategies...... for management and employees in a knowledge based organisation. Based on an action-research case study, we offer an example of how learning styles affects individual learning and thus personal knowledge creation in practice. The paper argues that innovation and knowledge management is enhanced and facilitated...... in practice by working with the learning styles of individuals and groups/teams....

  11. International Organisations and Transnational Education Policy

    DEFF Research Database (Denmark)

    Moutsios, Stavros

    2009-01-01

    This paper focuses on the World Bank/IMF (International Monetary Fund), the OECD (Organisation for Economic Cooperation and Development) and the WTO (World Trade Organisation) as institutions of transnational policy making. They are all at present making education policies which are decisively...... shaping current directions and developments in national education systems. The paper reviews the enhanced role of these institutions in producing education policies and investigates the ideological basis as well as the processes through which these policies are made. It is argued that decisions are taken...... the transnationalisation of education policy making but also the full submission of education to the pursuits of global economy....

  12. The man-machine-organisation interface

    International Nuclear Information System (INIS)

    The reliable and safety-centred action of man is one crucial factor for safe operation of nuclear power plants, apart from the technical systems and the organisational aspects. Essential factors influencing human performance are: Qualification and competence of the operating personnel, technical conditions and status of systems, including the level of automation, information technology in the control room, and plant organisation. Analyses of documentation of notifiable events in power plant operation or other incidents yield information on available potentials for safety enhancements or reduction of human errors. (orig./CB)

  13. The Organisational Gap Model for Hotel Management

    Directory of Open Access Journals (Sweden)

    Maja Uran

    2010-12-01

    Full Text Available This paper describes the development of the organisational gap model for hotel management. It descries a management measurement instrument that helps to assess the 3 organizational service gaps that are preconditions for delivering service quality (the positioning gap, specification gap and evaluation gap. The described theoretical model was constructed based upon the four organisational gaps of the Parasuraman et al. service quality model, then redefined and reassessed. Data were gathered on the sample of 500 questionnaires from the Slovenian hotel industry and analysed with exploratory factor analysis and structural equation modelling. The results can be useful guidelines for hotel management on how to improve the service delivery process.

  14. Measuring business continuity programmes in large organisations.

    Science.gov (United States)

    Green, Christopher

    2014-01-01

    In the field of business continuity management, organisations commit sums of money (often very large sums) to develop and maintain their continuity capability. Despite this, there is almost no measurement of whether this expense offers value for money, or whether it is targeted in the right areas. This paper will explain some methods of measuring components of a business continuity programme. The important outputs from this measurement activity are to demonstrate that an organisation's continuity capability is improving over time, and to identify areas of weakness that should be targeted during future work.

  15. Influencing organisational culture: a leadership challenge.

    Science.gov (United States)

    Muls, Ann; Dougherty, Lisa; Doyle, Natalie; Shaw, Clare; Soanes, Louise; Stevens, Anna-Marie

    In the wake of the Francis report, the need for NHS trusts and hospitals to adopt a culture of learning, safety and transparency has been highlighted. This article considers different aspects of culture in health care, and hones in on the link between culture and safety for patients in putting the patient first, embedding the 6Cs and considering the options to measure and influence organisational culture. The article reflects more deeply on how leadership across all levels can influence and inspire change in organisational culture, ensuring that the patient remains the focus of any changes in care delivery.

  16. A Wicked Problem? Whistleblowing in Healthcare Organisations

    Science.gov (United States)

    Hyde, Paula

    2016-01-01

    Mannion and Davies’ article recognises whistleblowing as an important means of identifying quality and safety issues in healthcare organisations. While ‘voice’ is a useful lens through which to examine whistleblowing, it also obscures a shifting pattern of uncertain ‘truths.’ By contextualising cultures which support or impede whislteblowing at an organisational level, two issues are overlooked; the power of wider institutional interests to silence those who might raise the alarm and changing ideas about what constitutes adequate care. A broader contextualisation of whistleblowing might illuminate further facets of this multi-dimensional problem. PMID:27239870

  17. Influencing organisational culture: a leadership challenge.

    Science.gov (United States)

    Muls, Ann; Dougherty, Lisa; Doyle, Natalie; Shaw, Clare; Soanes, Louise; Stevens, Anna-Marie

    In the wake of the Francis report, the need for NHS trusts and hospitals to adopt a culture of learning, safety and transparency has been highlighted. This article considers different aspects of culture in health care, and hones in on the link between culture and safety for patients in putting the patient first, embedding the 6Cs and considering the options to measure and influence organisational culture. The article reflects more deeply on how leadership across all levels can influence and inspire change in organisational culture, ensuring that the patient remains the focus of any changes in care delivery. PMID:26110855

  18. Familial complex chromosomal rearrangement resulting in a recombinant chromosome.

    Science.gov (United States)

    Berend, Sue Ann; Bodamer, Olaf A F; Shapira, Stuart K; Shaffer, Lisa G; Bacino, Carlos A

    2002-05-15

    Familial complex chromosomal rearrangements (CCRs) are rare and tend to involve fewer breakpoints and fewer chromosomes than CCRs that are de novo in origin. We report on a CCR identified in a child with congenital heart disease and dysmorphic features. Initially, the child's karyotype was thought to involve a straightforward three-way translocation between chromosomes 3, 8, and 16. However, after analyzing the mother's chromosomes, the mother was found to have a more complex rearrangement that resulted in a recombinant chromosome in the child. The mother's karyotype included an inverted chromosome 2 and multiple translocations involving chromosomes 3, 5, 8, and 16. No evidence of deletion or duplication that could account for the clinical findings in the child was identified.

  19. [Chromosomal organization of the genomes of small-chromosome plants].

    Science.gov (United States)

    Muravenko, O V; Zelenin, A V

    2009-11-01

    An effective approach to study the chromosome organization in genomes of plants with small chromosomes and/or with low-informative C-banding patterns was developed in the course of investigation of the karyotypes of cotton plant, camomile, flax, and pea. To increase the resolving power of chromosome analysis, methods were worked out for revealing early replication patterns on chromosomes and for artificial impairment of mitotic chromosome condensation with the use of a DNA intercalator, 9-aminoacridine (9-AMA). To estimate polymorphism of the patterns of C-banding of small chromosomes on preparations obtained with the use of 9-AMA, it is necessary to choose a length interval that must not exceed three average sizes of metaphase chromosomes without the intercalator. The use of 9-AMA increases the resolution of differential C- and OR-banding and the precision of physical chromosome mapping by the FISH method. Of particular importance in studying small chromosomes is optimization of the computer-aided methods used to obtain and process chromosome images. The complex approach developed for analysis of the chromosome organization in plant genomes was used to study the karyotypes of 24 species of the genus Linum L. It permitted their chromosomes to be identified for the first time, and, in addition, B chromosomes were discovered and studied in the karyotypes of the species of the section Syllinum. By similarity of the karyotypes, the studied flax species were distributed in eight groups in agreement with the clusterization of these species according to the results of RAPD analysis performed in parallel. Systematic positions and phylogenetic relationships of the studied flax species were verified. Out results can serve as an important argument in favour of the proposal to develop a special program for sequencing the genome of cultivated flax (L. usitatissimum L.), which is a major representative of small-chromosome species. PMID:20058798

  20. Anchoring genome sequence to chromosomes of the central bearded dragon (Pogona vitticeps) enables reconstruction of ancestral squamate macrochromosomes and identifies sequence content of the Z chromosome

    OpenAIRE

    Deakin, Janine E; Edwards, Melanie J; Patel, Hardip; O’Meally, Denis; Lian, Jinmin; Stenhouse, Rachael; Ryan, Sam; Livernois, Alexandra M.; Azad, Bhumika; Clare E Holleley; Li, Qiye; Georges, Arthur

    2016-01-01

    Background Squamates (lizards and snakes) are a speciose lineage of reptiles displaying considerable karyotypic diversity, particularly among lizards. Understanding the evolution of this diversity requires comparison of genome organisation between species. Although the genomes of several squamate species have now been sequenced, only the green anole lizard has any sequence anchored to chromosomes. There is only limited gene mapping data available for five other squamates. This makes it diffic...

  1. Information Systems in Organisations --organisational culture affects information systems in organizations

    Institute of Scientific and Technical Information of China (English)

    张娟

    2013-01-01

    In today’s information age, the application and construction of information systems became the central element of organizational development. Except the influence of information technology, the organisational culture plays a most important role in the implementation of information systems. This paper aimed at information producers, information users, information content and the information channels four parts of information elements to analysis the affects of organisational culture on information system in organisations. In particular, power distance, individualism and collectivism and uncertainty avoidance as three main theory models of Hofstede’s culture theory were be applied in this paper.

  2. "It Just Makes You Feel Invincible": A Foucauldian Analysis of Children's Experiences of Organised Team Sports

    Science.gov (United States)

    Walters, Simon R.; Payne, Deborah; Schluter, Philip J.; Thomson, Rex W.

    2015-01-01

    The childhood years are highlighted as a crucial time when ongoing participation in physical activity can be nurtured and maintained. The nurturing of a child's proclivity to participate in organised sport normally falls into the domain of adults. While both parents and coaches have been identified as key influences on children's…

  3. Quantifying the effects of resolution on convective organisation in cloud-system resolving simulations of West Africa.

    Science.gov (United States)

    White, Bethan; Stier, Philip; Birch, Cathryn

    2016-04-01

    Convection transports moisture, momentum, heat and aerosols through the troposphere, and so the variability of convection is a major driver of global weather and climate. Convection in the tropics is organised across a wide range of spatiotemporal scales, from the few kilometres and hours associated with individual cloud systems, through the mesoscale of squall lines and cloud clusters, to the synoptic scale of tropical cyclones. Global and limited area models often fail to represent many of these scales of organisation, and the interaction between the scales remains poorly understood. In this work we devise a new metric to quantify the degree of convective organisation. We apply this metric to data from simulations of the West African Monsoon region from the CASCADE project, where simulations were performed using the Met Office Unified Model at 12 km horizontal grid length with parameterised convection, and at 12, 4 and 1.5 km horizontal grid lengths with permitted convection. This allows us to perform quantitative analysis of convective organisation across model configurations that experience the same large-scale state and differ only in horizontal grid length and representation of deep convection. We show that our analysis technique can be usefully applied to high-resolution, cloud-system resolving, large-domain simulations of tropical convection. We use our technique to quantify the effects of horizontal grid length and of convective parameterisation on the degree of organisation in the simulated convection, and investigate the spatiotemporal variability of the convective organisation in the different model configurations. We then determine relationships between the degree of convective organisation and precipitation. Further, we compare our results against equivalent parameters derived from satellite data to identify how well each of the model configurations performs against observations. Through the use of this new metric, this work provides a quantitative

  4. Evaluating the internalisation of core values at a South African public service organisation

    Directory of Open Access Journals (Sweden)

    Susanna M. O’Neil

    2012-09-01

    Full Text Available Orientation: Fully entrenched and internalised organisational values have proved a competitive advantage for many leading organisations. The benefits range from higher profit margins to the improvement of employees’ commitment and ethical performance. Nevertheless, the process of value shaping is often no more than a management goal. It is rarely truly internalised by the whole organisation.Research purpose: This article presents an effort to describe a value internalisation effort within a South African public service organisation as well as the results of a subsequent evaluation to ascertain to what extent those efforts actually led to internalisation throughout the organisation. A set of actions and practices were implemented within the public service organisation; the intent was that they should enhance value internalisation in the organisation. A long-term strategy of value internalisation was followed that focussed mainly on the clear articulation and communication of the values through different communication mediums and platforms, such as road shows and branded value material hand-outs, as well as through extensive value internalisation training.Motivation for the study: Documentation of value internalisation processes and its evaluation, especially in South African public service organisations is extremely rare. To ensure that public service organisations do not repeat the same mistakes in their value internalisation practices and implementation processes, proper documentation of these processes in the public and research domains are needed. The need for the evaluation of value internalisation programmes should also be propagated as in many instances, programmes are implemented, but the subsequent success thereof is never evaluated.Research design, approach and method: A survey questionnaire consisting of a 5-point rating scale was developed to measure the extent of value internalisation after the implementation of long

  5. Resilience Safety Culture in Aviation Organisations

    NARCIS (Netherlands)

    Akselsson, R.; Koornneef, F.; Stewart, S.; Ward, M.

    2009-01-01

    Chapter 2: Resilience Safety Culture in Aviation Organisations The European Commission HILAS project (Human Integration into the Lifecycle of Aviation Systems - a project supported by the European Commission’s 6th Framework between 2005-2009) was focused on using human factors knowledge and methodo

  6. Ising, Schelling and Self-Organising Segregation

    CERN Document Server

    Stauffer, D

    2007-01-01

    The similarities between phase separation in physics and residential segregation by preference in the Schelling model of 1971 are reviewed. Also, new computer simulations of asymmetric interactions different from the usual Ising model are presented, showing spontaneous magnetisation (= self-organising segregation) and in one case a sharp phase transition.

  7. Ising, Schelling and self-organising segregation

    Science.gov (United States)

    Stauffer, D.; Solomon, S.

    2007-06-01

    The similarities between phase separation in physics and residential segregation by preference in the Schelling model of 1971 are reviewed. Also, new computer simulations of asymmetric interactions different from the usual Ising model are presented, showing spontaneous magnetisation (=self-organising segregation) and in one case a sharp phase transition.

  8. Management of Adult Education Organisations in Africa

    Science.gov (United States)

    Nafukho, Fredrick Muyia; Wawire, Nelson H. W.; Lam, Penina Mungania

    2011-01-01

    Adult education is now considered a mainstream academic discipline in several African countries, and its importance in today's knowledge and "ideas" economies is growing steadily. It is provided by organisations such as public universities, training colleges, corporate universities and employers. The successful operation of educational…

  9. VIRTUAL ORGANISATIONS: EMPLOYEE COMPETENCY AND MANAGERIAL ISSUES

    Directory of Open Access Journals (Sweden)

    MIRJANA RADOVIC-MARKOVIC

    2014-10-01

    Full Text Available New technologies have led to a new information and knowledge based economy. In this context, technology has changed the work environment, where organisations have become increasingly complex and competitive. Namely, the technologically induced a “virtual” environment has resulted in the adoption of new organisational structures and work skills and practices. On the one hand, the workplace increasingly requires employee to work in teams,collaborating across companies, communities, and continents. These changes and the new organisational structures have also made an impact on role of managers and their management styles, on the other hand. In line with this, there a very rich collection of thinking and empirical research findings on the subject. The goal of our research was to recognize the importance of certain factors in motivating employees in organisations by managers . The other purpose was to investigate the job related motivation factors among senior and junior employees as well as explore issues in the workplace that may affect work demoralization. Furthemore, we explored the relationship between employees motivation and their competences.We also contributed to the topic in our research project-book with the new model of competency. Furthemore, we expect that our methodology will be implemented in practice. However, it should be also a good basis for further improvements in this area.

  10. On the organisation of program verification competitions

    NARCIS (Netherlands)

    Huisman, Marieke; Klebanov, Vladimir; Monahan, Rosemary; Klebanov, Vladimir; Beckert, Bernhard; Biere, Armin; Sutcliffe, Geoff

    2012-01-01

    In this paper, we discuss the challenges that have to be addressed when organising program verification competitions. Our focus is on competitions for verification systems where the participants both formalise an informally stated requirement and (typically) provide some guidance for the tool to sho

  11. The corporate republic. Complex organisations and citizenship

    NARCIS (Netherlands)

    Bovens, M.A.P.

    1998-01-01

    The twentieth century has been the century of complex organisations. However, most contemporary political currents act as if we were still living in the nineteenth century. For many modern liberals, social democrats, and communitarians, the contrast between public and private, between government and

  12. The institutional dynamics of voluntary organisations

    DEFF Research Database (Denmark)

    Aagaard, Peter

    What features of institutional change do voluntary organisations contain? This question is debated in the civil society literature, but often under different headlines, like social entrepreneurship or social movement theory. The question of voluntarism is often not taken into account. This paper...

  13. Labour organisation on robotic milking dairy farms.

    NARCIS (Netherlands)

    Sonck, B.R.

    1996-01-01

    1. Research issuesThe research described in this dissertation is focused on the effects of the integration of the milking robot in a dairy farm on the labour organisation at operational and tactical level. Attention was paid to the future requirements concerning hum

  14. Defining, Measuring, and Comparing Organisational Cultures

    NARCIS (Netherlands)

    Berg, van den Peter T.; Wilderom, Celeste P.M.

    2004-01-01

    La littérature portant sur la culture des organisations souffre d’un manque manifeste d’enquêtes extensives débouchant sur des études comparatives. Afin de rendre plus comparables les cultures organisationnelles, nous proposons une définition et une série de dimensions. La culture organisationnelle

  15. Institutional Level Student Engagement and Organisational Cultures

    Science.gov (United States)

    van der Velden, Gwen

    2012-01-01

    Driven by the growing presence of market forces within higher education worldwide, universities are changing the way they engage with students. This article explores how a university's internal culture relates to engagement with students and their views. It builds on wider research into student engagement and organisational cultures. The…

  16. Teamarbejde og samspillet mellem medarbejdere og organisation

    DEFF Research Database (Denmark)

    Jeppesen, Hans Jeppe; Jønsson, Thomas

    2008-01-01

    teamwork. Bag projektet står professor Hans Jeppe Jeppesen, cand.psych. og ph.d. Thomas Rasmussen samt adjunkt Thomas Jønsson. I denne klumme gives en forskningsbaseret introduktion til teamarbejdet og dets betydning for samspillet mellem medarbejdere og organisation. Med udgangspunkt i egen forskning...

  17. Learning, Learning Organisations and the Global Enterprise

    Science.gov (United States)

    Manikutty, Sankaran

    2009-01-01

    The steadily increasing degree of globalisation of enterprises implies development of many skills, among which the skills to learn are among the most important. Learning takes place at the individual level, but collective learning and organisational learning are also important. Learning styles of individuals are different and learning styles are…

  18. Construction of human chromosome 21-specific yeast artificial chromosomes.

    Science.gov (United States)

    McCormick, M K; Shero, J H; Cheung, M C; Kan, Y W; Hieter, P A; Antonarakis, S E

    1989-12-01

    Chromosome 21-specific yeast artificial chromosomes (YACs) have been constructed by a method that performs all steps in agarose, allowing size selection by pulsed-field gel electrophoresis and the use of nanogram to microgram quantities of DNA. The DNA sources used were hybrid cell line WAV-17, containing chromosome 21 as the only human chromosome and flow-sorted chromosome 21. The transformation efficiency of ligation products was similar to that obtained in aqueous transformations and yielded YACs with sizes ranging from 100 kilobases (kb) to greater than 1 megabase when polyamines were included in the transformation procedure. Twenty-five YACs containing human DNA have been obtained from a mouse-human hybrid, ranging in size from 200 to greater than 1000 kb, with an average size of 410 kb. Ten of these YACs were localized to subregions of chromosome 21 by hybridization of RNA probes (corresponding to the YAC ends recovered in Escherichia coli) to a panel of somatic cell hybrid DNA. Twenty-one human YACs, ranging in size from 100 to 500 kb, with an average size of 150 kb, were obtained from approximately equal to 50 ng of flow-sorted chromosome 21 DNA. Three were localized to subregions of chromosome 21. YACs will aid the construction of a physical map of human chromosome 21 and the study of disorders associated with chromosome 21 such as Alzheimer disease and Down syndrome.

  19. Antecedents of Organisational Creativity: A Multi-Level Approach

    Directory of Open Access Journals (Sweden)

    Ritu Gupta

    2016-06-01

    Full Text Available The purpose of this literature review is to provide a better understanding of the antecedents of organisational creativity with a multi-level approach. Organisational creativity is a sum total of the creativity accounted for by the individual employees of the organisation, the cumulative creativity of a team or group and creativity arising out of different structural components of an organisation. Some of the antecedents identified from the literature include personality, intrinsic motivation, group cohesion, social inhibition, cognitive interference, leader member exchange, organisational culture and climate, amongst others at individual, group and organisational level. Based on the literature review, suggestions for future research and research propositions have been proposed.

  20. The emergence of user organisation of homeless persons in Denmark

    DEFF Research Database (Denmark)

    Anker, Jørgen

    persons emerges in these years. The second part of the paper provides a more detailed description of the organisation, its aims, strategies and organisational structure. Some of the dilemmas and difficulties of the user organisation are finally mentioned at the end of the paper.......This paper provides an overview of the history of the user organisation of homeless persons in Denmark. The first part of the paper seeks to identify some of the processes and actors who facilitated the formation of the organisation, and it seeks to discuss why a user organisation of homeless...

  1. Influencing organisational culture by means of employee remuneration

    Directory of Open Access Journals (Sweden)

    Katarína Stachová

    2015-10-01

    Full Text Available The aim of this paper is to particularly define the possibilities of influencing organisational culture by means of employee remuneration. The paper will present the results of research conducted over 2010–2012. The respondent group included 340 organisations operating in the Slovak Republic. The research showed that even though 98% of the interviewed organisations realise the importance and meaning of dealing with the creation and maintenance of organisational culture, only 21–43% have defined an organisational culture strategy in writing within the organisation´s strategy.

  2. ORGANISATIONAL JUSTICE AND AFFECTIVE COMMITMENT: THE MEDIATING ROLE OF PERCEIVED ORGANISATIONAL SUPPORT

    Directory of Open Access Journals (Sweden)

    Pooja Purang

    2011-01-01

    Full Text Available Perception of fairness among employees is a guiding force that influences employee attitude and behaviour. However, the mechanisms associated with justice need to be studied further. This study hypothesises that perceived organisational support (POS mediates the relationship between organisational justice perceptions and affective commitment of employees. The study was conducted in a multi-national organisation operating in India in the service sector; the sample size was 71 employees. Baron and Kenny's model of studying the mediating relationship was used. The findings showed that POS fully mediates the relationship between distributive justice and affective commitment as well as partially mediates the relationship between procedural justice and affective commitment. This study highlights the importance of fairness and justice in organisations and identifies the mechanism by which employee perceptions of justice influence their loyalty and involvement.

  3. ISO 9000 IMPLEMENTATION AND PERCEIVED ORGANISATIONAL OUTCOME: THE CASE OF A SERVICE ORGANISATION

    Directory of Open Access Journals (Sweden)

    Jasni Abdul Latif

    2003-01-01

    Full Text Available The study examines the relationship between the perceptions of the employees about ISO 9000 implementation and perceived organisational outcomes. A conceptual framework linking the level of employee influence and involvement, their perception of ISO 9000 and the perceived impact on their work and the organisational outcomes is posited. Data was collected from a random sample of 64 employees of a service organisation. Contrary to expectations, the work impact of ISO 9000 was not correlated with the efficiency and market outcomes of ISO 9000 certification but the employee involvement, influence and perception of ISO 9000 were. The conceptualization of the influence path of ISO 9000 implementation on the organisational outcomes may require rethinking as perception may have a direct effect on the outcomes without the mediation of the impact on work. In short, employees may report benefits or positive outcomes even though no change is reported in their work.

  4. European ways to combat psychosocial risks related to work organisation : towards organisational interventions?

    OpenAIRE

    Oeij, P.R.A.; Morvan, E.; Houtman, I.L.D.; Vaas, F.; Wiezer, N.

    2004-01-01

    From 24-26 November 2004, the 6h Annual Conference of the European Academy of Occupational Health Psychology ‘Healthy, Efficient & Productive Organisations’ was held in Oporto, Portugal. During this conference, the Workshop ‘Organisational interventions to combat psychosocial factors of stress’ was organised by PEROSH, Partnership for European Research in Occupational Safety and Health. This syllabus contains the separate papers of various European countries that were delivered to the worksho...

  5. Caseload midwifery as organisational change: the interplay between professional and organisational projects in Denmark

    OpenAIRE

    Burau, Viola; Overgaard, Charlotte

    2015-01-01

    Background The large obstetric units typical of industrialised countries have come under criticism for fragmented and depersonalised care and heavy bureaucracy. Interest in midwife-led continuity models of care is growing, but knowledge about the accompanying processes of organisational change is scarce. This study focuses on midwives’ role in introducing and developing caseload midwifery. Sociological studies of midwifery and organisational studies of professional groups were used to capture...

  6. Involvement of condensin-directed gene associations in the organization and regulation of chromosome territories during the cell cycle

    Science.gov (United States)

    Iwasaki, Osamu; Corcoran, Christopher J.; Noma, Ken-ichi

    2016-01-01

    Chromosomes are not randomly disposed in the nucleus but instead occupy discrete sub-nuclear domains, referred to as chromosome territories. The molecular mechanisms that underlie the formation of chromosome territories and how they are regulated during the cell cycle remain largely unknown. Here, we have developed two different chromosome-painting approaches to address how chromosome territories are organized in the fission yeast model organism. We show that condensin frequently associates RNA polymerase III-transcribed genes (tRNA and 5S rRNA) that are present on the same chromosomes, and that the disruption of these associations by condensin mutations significantly compromises the chromosome territory arrangement. We also find that condensin-dependent intra-chromosomal gene associations and chromosome territories are co-regulated during the cell cycle. For example, condensin-directed gene associations occur to the least degree during S phase, with the chromosomal overlap becoming largest. In clear contrast, condensin-directed gene associations become tighter in other cell-cycle phases, especially during mitosis, with the overlap between the different chromosomes being smaller. This study suggests that condensin-driven intra-chromosomal gene associations contribute to the organization and regulation of chromosome territories during the cell cycle. PMID:26704981

  7. Reorganization of chromosome architecture in replicative cellular senescence.

    Science.gov (United States)

    Criscione, Steven W; De Cecco, Marco; Siranosian, Benjamin; Zhang, Yue; Kreiling, Jill A; Sedivy, John M; Neretti, Nicola

    2016-02-01

    Replicative cellular senescence is a fundamental biological process characterized by an irreversible arrest of proliferation. Senescent cells accumulate a variety of epigenetic changes, but the three-dimensional (3D) organization of their chromatin is not known. We applied a combination of whole-genome chromosome conformation capture (Hi-C), fluorescence in situ hybridization, and in silico modeling methods to characterize the 3D architecture of interphase chromosomes in proliferating, quiescent, and senescent cells. Although the overall organization of the chromatin into active (A) and repressive (B) compartments and topologically associated domains (TADs) is conserved between the three conditions, a subset of TADs switches between compartments. On a global level, the Hi-C interaction matrices of senescent cells are characterized by a relative loss of long-range and gain of short-range interactions within chromosomes. Direct measurements of distances between genetic loci, chromosome volumes, and chromatin accessibility suggest that the Hi-C interaction changes are caused by a significant reduction of the volumes occupied by individual chromosome arms. In contrast, centromeres oppose this overall compaction trend and increase in volume. The structural model arising from our study provides a unique high-resolution view of the complex chromosomal architecture in senescent cells. PMID:26989773

  8. Chromosomal localization of the human and mouse hyaluronan synthase genes

    Energy Technology Data Exchange (ETDEWEB)

    Spicer, A.P.; McDonald, J.A. [Mayo Clinic Scottsdale, AZ (United States); Seldin, M.F. [Univ. of California Davis, CA (United States)] [and others

    1997-05-01

    We have recently identified a new vertebrate gene family encoding putative hyaluronan (HA) synthases. Three highly conserved related genes have been identified, designated HAS1, HAS2, and HAS3 in humans and Has1, Has2, and Has3 in the mouse. All three genes encode predicted plasma membrane proteins with multiple transmembrane domains and approximately 25% amino acid sequence identity to the Streptococcus pyogenes HA synthase, HasA. Furthermore, expression of any one HAS gene in transfected mammalian cells leads to high levels of HA biosynthesis. We now report the chromosomal localization of the three HAS genes in human and in mouse. The genes localized to three different positions within both the human and the mouse genomes. HAS1 was localized to the human chromosome 19q13.3-q13.4 boundary and Has1 to mouse Chr 17. HAS2 was localized to human chromosome 8q24.12 and Has2 to mouse Chr 15. HAS3 was localized to human chromosome 16q22.1 and Has3 to mouse Chr 8. The map position for HAS1 reinforces the recently reported relationship between a small region of human chromosome 19q and proximal mouse chromosome 17. HAS2 mapped outside the predicted critical region delineated for the Langer-Giedion syndrome and can thus be excluded as a candidate gene for this genetic syndrome. 33 refs., 2 figs.

  9. Organisational learning won't be turned off

    DEFF Research Database (Denmark)

    Elkjær, Bente

    2012-01-01

    individuals and environments, which is my reason for including a section on just that. I draw the two, learning and organisation, together in a concluding section in which there is also a discussion about how a focus on organisational learning can inspire the research on individual learning.......Enterprises have always been learning organisations in much the same way as it is hard for people to avoid learning. A sign of learning organisations are the many successful enterprises, and the abundance of new products and services that keeps emerging. The question about organisational learning...... is how it is possible to ‘see’ and to analyse learning when learning is not connected to individuals but to organisations and organising? In a normative sense, however, “organisational learning” is often used as our answer to the difficulties in enterprises of how most efficiently to channel knowledge...

  10. Proceedings Second Workshop on Formal Aspects of Virtual Organisations

    OpenAIRE

    Bryans, Jeremy; Fitzgerald, John

    2010-01-01

    FAVO2009 was the second workshop on Formal Aspects of Virtual Organisations. The purpose of the FAVO workshops is to encourage an active community of researchers and practitioners using formal methods in the research and development of Virtual Organisations.

  11. The Concept of Embodied Knowledge for Understanding Organisational Knowledge Creation

    Science.gov (United States)

    Matsudaira, Yoshito; Fujinami, Tsutomu

    Our goal in this paper is to understand, in the light of intuition and emotion, the problem-finding and value judgments by organisational members that are part of organisational knowledge creation. In doing so, we emphasise the importance of embodied knowledge of organisations as an explanatory concept. We propose ways of approaching intuition and sense of value as these are posited as objects of research. Approaches from the first, second, and third-person viewpoints result in a deeper grasp of embodied knowledge of organisations. Important in organisational knowledge creation is embodied knowledge of organisations, which has a bearing on problem-finding before any problem-solving or decision making takes place, and on value judgments about the importance of problems that have been found. This article proposes the concept of embodied knowledge, and, by introducing it, gives a profound understanding of that facet of organisational knowledge creation characterised by tacit knowledge held by organisational individuals.

  12. Structural and functional diversity of Topologically Associating Domains.

    Science.gov (United States)

    Dekker, Job; Heard, Edith

    2015-10-01

    Recent studies have shown that chromosomes in a range of organisms are compartmentalized in different types of chromatin domains. In mammals, chromosomes form compartments that are composed of smaller Topologically Associating Domains (TADs). TADs are thought to represent functional domains of gene regulation but much is still unknown about the mechanisms of their formation and how they exert their regulatory effect on embedded genes. Further, similar domains have been detected in other organisms, including flies, worms, fungi and bacteria. Although in all these cases these domains appear similar as detected by 3C-based methods, their biology appears to be quite distinct with differences in the protein complexes involved in their formation and differences in their internal organization. Here we outline our current understanding of such domains in different organisms and their roles in gene regulation. PMID:26348399

  13. Trusted Domain

    DEFF Research Database (Denmark)

    Hjorth, Theis Solberg; Torbensen, Rune

    2012-01-01

    In the digital age of home automation and with the proliferation of mobile Internet access, the intelligent home and its devices should be accessible at any time from anywhere. There are many challenges such as security, privacy, ease of configuration, incompatible legacy devices, a wealth...... of wireless standards, limited resources of embedded systems, etc. Taking these challenges into account, we present a Trusted Domain home automation platform, which dynamically and securely connects heterogeneous networks of Short-Range Wireless devices via simple non-expert user. interactions, and allows...... that enables secure end-to-end communication with home automation devices, and it supports device revocations as well as a structure of intersecting sets of nodes for scalability. Devices in the Trusted Domain are registered in a list that is distributed using a robust epidemic protocol optimized...

  14. Digital Ecosystems: Self-Organisation of Evolving Agent Populations

    OpenAIRE

    Briscoe, Gerard; De Wilde, Philippe

    2008-01-01

    A primary motivation for our research in Digital Ecosystems is the desire to exploit the self-organising properties of biological ecosystems. Ecosystems are thought to be robust, scalable architectures that can automatically solve complex, dynamic problems. Self-organisation is perhaps one of the most desirable features in the systems that we engineer, and it is important for us to be able to measure self-organising behaviour. We investigate the self-organising aspects of Digital Ecosystems, ...

  15. 11th International Symposium on Organisational Science Development

    OpenAIRE

    Aleksander Janes

    2008-01-01

    In this paper we present the organisation and execution of the 11th International Symposium on Organisational Science Development, entitled Management and Social Responsibility, which is organised by the Faculty of Organisational Sciences in Belgrade. The Symposium is already a traditional event and it has taken place in Zlatibor for many years, but this year it was held in Belgrade itself in order to celebrate the 200th anniversary of the University of Belgrade. The fact that more than 450 a...

  16. An ethics model to develop an ethical organisation

    OpenAIRE

    Hendrik R. Lloyd; Michelle R. Mey

    2010-01-01

    Orientation: As background to the study it can be stated that the ethical conduct of employees in an organisation is of paramount importance to the successful operations of an organisation, both real and perceived. In recent times the ethical conduct of employees has received extensive publicity and, as such, has emphasised the impact of organisational ethics on the global competitiveness of organisations.Research purpose: The main aim of the paper is to analyse the impact of business ethics ...

  17. The Importance of Business Rules in the Organisational Transformation Process

    OpenAIRE

    Stergiou, Maria; Johnson, Leslie

    1998-01-01

    Organisational transformation has been widely discussed and practised. Organisations tried and tested many ways of collecting requirements and planning their change programmes. In this paper we discuss the fuller role that the requirements specification stage is called to play in organisational transformation. A role that encircles an understanding of the business rules of the organisation, both written and unwritten. We state the implications of our analysis to the infamous Business-IT gap a...

  18. Shaping communities of work, an organisational development approach

    OpenAIRE

    Schoemaker, M.J.R.

    2006-01-01

    "In order to survive in a turbulent environment many organisations have to operate as an open system. This open system often has the configuration of a network. This networked organisation acts as a community of work. This community of work is based upon a specific organisational identity. The central question addressed by this article is: How can organisation development function in the process of developing communities of work and what is the role of the change agent? T...

  19. Organisational learning and transformative capacity: leveraging collective knowledge

    OpenAIRE

    Shehata, Gamal Mohamed Mohamed

    2000-01-01

    A growing part of the literature on organisational learning sets out to describe how and why organisations learn. Nevertheless, a large number of the recent reviews on organisational learning address several gaps that challenge the future progress of the subject. A critical examination of the organisational learning literature was conducted to identify a number of gaps that exist in the field. These gaps include the fragmented nature of the subject itself, the fact that insufficient attention...

  20. Influencing organisational culture by means of employee remuneration

    OpenAIRE

    Katarína Stachová; Zdenko Stacho; Gabriela Bartáková

    2015-01-01

    The aim of this paper is to particularly define the possibilities of influencing organisational culture by means of employee remuneration. The paper will present the results of research conducted over 2010–2012. The respondent group included 340 organisations operating in the Slovak Republic. The research showed that even though 98% of the interviewed organisations realise the importance and meaning of dealing with the creation and maintenance of organisational culture, only 21–43% have defin...

  1. Intraspecific chromosome variability

    Directory of Open Access Journals (Sweden)

    N Dubinin

    2010-12-01

    Full Text Available (Editorial preface. The publication is presented in order to remind us of one of dramatic pages of the history of genetics. It re-opens for the contemporary reader a comprehensive work marking the priority change from plant cytogenetics to animal cytogenetics led by wide population studies which were conducted on Drosophila polytene chromosomes. The year of the publication (1937 became the point of irretrievable branching between the directions of Old World and New World genetics connected with the problems of chromosome variability and its significance for the evolution of the species. The famous book of T. Dobzhansky (1937 was published by Columbia University in the US under the title “Genetics and the origin of species”, and in the shadow of this American ‘skybuilding’ all other works grew dim. It is remarkable that both Dobzhansky and Dubinin come to similar conclusions about the role of chromosomes in speciation. This is not surprising given that they both might be considered as representatives of the Russian genetic school, by their birth and education. Interestingly, Dobzhansky had never referred to the full paper of Dubinin et al. (1937, though a previous short communication in Nature (1936 was included together with all former papers on the related subject. In full, the volume of the original publication printed in the Biological Journal in Moscow comprised 47 pages, in that number 41 pages of the Russian text accompanied by 16 Figs, a table and reference list, and, above all, 6 pages of the English summary. This final part in English is now reproduced in the authors’ version with the only addition being the reference list in the originally printed form.

  2. Chromosome assortment in Saccharum.

    Science.gov (United States)

    Al-Janabi, S M; Honeycutt, R J; Sobral, B W

    1994-12-01

    Recent work has revealed random chromosome pairing and assortment in Saccharum spontaneum L., the most widely distributed, and morphologically and cytologically variable of the species of Saccharum. This conclusion was based on the analysis of a segregating population from across between S. spontaneum 'SES 208' and a spontaneously-doubled haploid of itself, derived from anther culture. To determine whether polysomic inheritance is common in Saccharum and whether it is observed in a typical biparental cross, we studied chromosome pairing and assortment in 44 progeny of a cross between euploid, meiotically regular, 2n=80 forms of Saccharum officinarum 'LA Purple' and Saccharum robustum ' Mol 5829'. Papuan 2n=80 forms of S. robustum have been suggested as the immediate progenitor species for cultivated sugarcane (S. officinarum). A total of 738 loci in LA Purple and 720 loci in Mol 5829 were amplified and typed in the progeny by arbitrarily primed PCR using 45 primers. Fifty and 33 single-dose polymorphisms were identified in the S. officinarum and S. robustum genomes, respectively (χ 2 at 98%). Linkage analysis of single-dose polymorphisms in both genomes revealed linkages in repulsion and coupling phases. In the S. officinarum genome, a map hypothesis gave 7 linkage groups with 17 linked and 33 unlinked markers. Four of 13 pairwise linkages were in repulsion phase and 9 were in coupling phase. In the S. robustum genome, a map hypothesis gave 5 linkage groups, defined by 12 markers, with 21 markers unlinked, and 2 of 9 pairwise linkages were in repulsion phase. Therefore, complete polysomic inheritance was not observed in either species, suggesting that chromosomal behavior is different from that observed by linkage analysis of over 500 markers in the S. spontaneum map. Implications of this finding for evolution and breeding are discussed.

  3. Organisation and sequence determination of glutamine-dependent carbamoyl phosphate synthetase II in Toxoplasma gondii.

    Science.gov (United States)

    Fox, Barbara A; Bzik, David J

    2003-01-01

    Carbamoyl phosphate synthetase II encodes the first enzymic step of de novo pyrimidine biosynthesis. Carbamoyl phosphate synthetase II is essential for Toxoplasma gondii replication and virulence. In this study, we characterised the primary structure of a 28kb gene encoding Toxoplasma gondii carbamoyl phosphate synthetase II. The carbamoyl phosphate synthetase II gene was interrupted by 36 introns. The predicted protein encoded by the 37 carbamoyl phosphate synthetase II exons was a 1,687 amino acid polypeptide with an N-terminal glutamine amidotransferase domain fused with C-terminal carbamoyl phosphate synthetase domains. This bifunctional organisation of carbamoyl phosphate synthetase II is unique, so far, to protozoan parasites from the phylum Apicomplexa (Plasmodium, Babesia, Toxoplasma) or zoomastigina (Trypanosoma, Leishmania). Apicomplexan parasites possessed the largest carbamoyl phosphate synthetase II enzymes due to insertions in the glutamine amidotransferase and carbamoyl phosphate synthetase domains that were not present in the corresponding gene segments from bacteria, plants, fungi and mammals. The C-terminal allosteric regulatory domain, the carbamoyl phosphate synthetase linker domain and the oligomerisation domain were also distinct from the corresponding domains in other species. The novel C-terminal regulatory domain may explain the lack of activation of Toxoplasma gondii carbamoyl phosphate synthetase II by the allosteric effector 5-phosphoribosyl 1-pyrophosphate. Toxoplasma gondii growth in vitro was markedly inhibited by the glutamine antagonist acivicin, an inhibitor of glutamine amidotransferase activity typically associated with carbamoyl phosphate synthetase II, guanosine monophosphate synthetase, or CTP synthetase. PMID:12547350

  4. How Can Organisations Learn: An Information Systems Development Perspective

    Science.gov (United States)

    Baxter, Gavin J.; Connolly, Thomas M.; Stansfield, Mark

    2009-01-01

    This article sets out to argue that organisations can learn but that they cannot do so in their own right without infrastructural support. This article further examines the notion that individuals in organisations also require the cognitive participation of the organisation itself as a learning entity to learn. The close reliance and affiliation…

  5. Knowledge Creation in Construction Organisations: A Case Approach

    Science.gov (United States)

    Eliufoo, Harriet

    2008-01-01

    Purpose: The purpose of this paper is to investigate and characterise the knowledge creation process in construction organisations and explore to what extent organisations facilitate the process. Design/methodology/approach: A case study approach is adopted using four construction organisations; a knowledge creation model is also used as the…

  6. Bridging Theory and Practice: A Conceptual Framework for Consulting Organisations

    Science.gov (United States)

    Mohammed, Meca B.; Welch, Jennie; Hazle Bussey, Leslie

    2015-01-01

    A growing number of organisations are emerging as partners to districts pursuing systemic improvement. Given the critical role a consulting organisation could play in supporting system reform efforts, how does a district leader looking to establish a consulting partnership determine what characteristics in a consulting organisation may be more…

  7. Organisational citizens or reciprocal relationships? An empirical comparison

    NARCIS (Netherlands)

    Koster, Ferry; Sanders, Karin

    2006-01-01

    Purpose: This paper aims at contributing to the debate on organisational citizenship behaviour (OCB) by developing a theory‐driven measure of cooperative behaviour within organisations, called organisational solidarity (OS). Design/methodology/approach: Data are gathered through a survey among 674

  8. Interlingual Lexical Organisation for Multilingual Lexical Databases in NADIA

    OpenAIRE

    Sérasset, Gilles

    1995-01-01

    We propose a lexical organisation for multilingual lexical databases (MLDB). This organisation is based on acceptions (word-senses). We detail this lexical organisation and show a mock-up built to experiment with it. We also present our current work in defining and prototyping a specialised system for the management of acception-based MLDB. Keywords: multilingual lexical database, acception, linguistic structure.

  9. Organisational mergers: a behavioural perspective on identity management

    NARCIS (Netherlands)

    S.R. Giessner (Steffen)

    2016-01-01

    markdownabstractOrganisational mergers are one of the most extreme forms of organisational change processes. Consequently, they often result in difficulties for employees to adjust to the altered organisational conditions. This is often reflected in low levels of employee identification with the p

  10. Organisational Values in Higher Education: Perceptions and Preferences of Staff

    Science.gov (United States)

    Kleijnen, Jan; Dolmans, Diana; Muijtjens, Arno; Willems, Jos; Van Hout, Hans

    2009-01-01

    In this paper, staff members' perceptions about the organisational culture are measured. The questions addressed are: what are their opinions about the current and preferred organisational culture? Are there differences between the current and preferred situation? Do the perceptions differ per department? The Organisational Culture Assessment…

  11. The change capacity of organisations: general assessment and five configurations

    NARCIS (Netherlands)

    Bennebroek Gravenhorst, K.M.; Werkman, R.A.; Boonstra, J.J.

    2003-01-01

    Realizing major organisational change and innovation is a complex process and many organisations do not obtain the outcomes they desire. The purpose of this study is to investigate which factors hinder or contribute to far-reaching change. These factors are sought in characteristics of organisations

  12. Learning about Learning: Action Learning in Times of Organisational Change

    Science.gov (United States)

    Hill, Robyn

    2009-01-01

    This paper explores the conduct and outcomes of an action learning activity during a period of intense organisational change in a medium-sized vocational education and training organisation in Victoria, Australia. This organisation was the subject of significant change due to government-driven and statewide amalgamation, downsizing and sector…

  13. Ki-67 acts as a biological surfactant to disperse mitotic chromosomes.

    Science.gov (United States)

    Cuylen, Sara; Blaukopf, Claudia; Politi, Antonio Z; Müller-Reichert, Thomas; Neumann, Beate; Poser, Ina; Ellenberg, Jan; Hyman, Anthony A; Gerlich, Daniel W

    2016-07-14

    Eukaryotic genomes are partitioned into chromosomes that form compact and spatially well-separated mechanical bodies during mitosis. This enables chromosomes to move independently of each other for segregation of precisely one copy of the genome to each of the nascent daughter cells. Despite insights into the spatial organization of mitotic chromosomes and the discovery of proteins at the chromosome surface, the molecular and biophysical bases of mitotic chromosome structural individuality have remained unclear. Here we report that the proliferation marker protein Ki-67 (encoded by the MKI67 gene), a component of the mitotic chromosome periphery, prevents chromosomes from collapsing into a single chromatin mass after nuclear envelope disassembly, thus enabling independent chromosome motility and efficient interactions with the mitotic spindle. The chromosome separation function of human Ki-67 is not confined within a specific protein domain, but correlates with size and net charge of truncation mutants that apparently lack secondary structure. This suggests that Ki-67 forms a steric and electrostatic charge barrier, similar to surface-active agents (surfactants) that disperse particles or phase-separated liquid droplets in solvents. Fluorescence correlation spectroscopy showed a high surface density of Ki-67 and dual-colour labelling of both protein termini revealed an extended molecular conformation, indicating brush-like arrangements that are characteristic of polymeric surfactants. Our study thus elucidates a biomechanical role of the mitotic chromosome periphery in mammalian cells and suggests that natural proteins can function as surfactants in intracellular compartmentalization. PMID:27362226

  14. Ki-67 acts as a biological surfactant to disperse mitotic chromosomes.

    Science.gov (United States)

    Cuylen, Sara; Blaukopf, Claudia; Politi, Antonio Z; Müller-Reichert, Thomas; Neumann, Beate; Poser, Ina; Ellenberg, Jan; Hyman, Anthony A; Gerlich, Daniel W

    2016-06-29

    Eukaryotic genomes are partitioned into chromosomes that form compact and spatially well-separated mechanical bodies during mitosis. This enables chromosomes to move independently of each other for segregation of precisely one copy of the genome to each of the nascent daughter cells. Despite insights into the spatial organization of mitotic chromosomes and the discovery of proteins at the chromosome surface, the molecular and biophysical bases of mitotic chromosome structural individuality have remained unclear. Here we report that the proliferation marker protein Ki-67 (encoded by the MKI67 gene), a component of the mitotic chromosome periphery, prevents chromosomes from collapsing into a single chromatin mass after nuclear envelope disassembly, thus enabling independent chromosome motility and efficient interactions with the mitotic spindle. The chromosome separation function of human Ki-67 is not confined within a specific protein domain, but correlates with size and net charge of truncation mutants that apparently lack secondary structure. This suggests that Ki-67 forms a steric and electrostatic charge barrier, similar to surface-active agents (surfactants) that disperse particles or phase-separated liquid droplets in solvents. Fluorescence correlation spectroscopy showed a high surface density of Ki-67 and dual-colour labelling of both protein termini revealed an extended molecular conformation, indicating brush-like arrangements that are characteristic of polymeric surfactants. Our study thus elucidates a biomechanical role of the mitotic chromosome periphery in mammalian cells and suggests that natural proteins can function as surfactants in intracellular compartmentalization.

  15. Chromosome territories, X;Y translocation and Premature Ovarian Failure: is there a relationship?

    Directory of Open Access Journals (Sweden)

    Betri Enrico

    2009-09-01

    Full Text Available Abstract Background Premature ovarian failure (POF is a secondary hypergonadotrophic amenorrhea occurring before the age of 40 and affecting 1-3% of females. Chromosome anomalies account for 6-8% of POF cases, but only few cases are associated with translocations involving X and Y chromosomes. This study shows the cytogenetic and molecular analysis of a POF patient came to our attention as she developed a left ovary choriocarcinoma at the age of 10 and at 14 years of age she presented secondary amenorrhea with elevated levels of gonadotropins. Results Breakpoint position on X and Y chromosomes was investigated using Fluorescent In Situ Hybridisation (FISH with a panel of specific BAC probes, microsatellite analysis and evaluation of copy number changes and loss of heterozigosity by Affymetrix® GeneChip platform (Santa Clara, CA, USA. Patient's karyotype resulted 46, X, der(Yt(X;Y(q13.1;q11.223. X inactivation study was assessed by RBA banding and showed preferential inactivation of derivative chromosome. The reciprocal spatial disposition of sexual chromosome territories was investigated using whole chromosome painting and centromeres probes: patient's results didn't show a significant difference in comparison to normal controls. Conclusion The peculiar clinical case come to our attention highlighted the complexity of POF aetiology and of the translocation event, even if our results seem to exclude any effect on nuclear organisation. POF phenotype could be partially explained by skewed X chromosome inactivation that influences gene expression.

  16. Organising for power: solidarities and transformation

    Directory of Open Access Journals (Sweden)

    James Goodman

    2009-08-01

    Full Text Available Political and social movements are both empowering and power-seeking: they seek both to mobilize civil society and overwhelm state institutions. As organisations they mobilize collective power, generating solidarities and transforming social structures. As such, political organisations both challenge power and exercise power. This article addresses organizational vehicles for political change in Australia, drawing out limits and possibilities. Three organizational forms are discussed - the political party, the non-government organization (NGO, and the social movement - in terms of their capacity and limits. The social solidarities and social structures that frame political organization are debated, highlighting the impact of political conflicts over ecological change. The article ends with a discussion of the proceeding four articles, drawing out shared themes and implications in terms of the relationships post-Howard, between the Australian state, political parties, NGOs and movements.

  17. Exercises of first responder organisations in Austria

    Energy Technology Data Exchange (ETDEWEB)

    Schoenhacker, S. [WIRK.ZONE CBRN Preparedness and Response, Vienna (Austria)

    2011-12-15

    Although there is no nuclear industry in Austria, there are still many possible scenarios which require proper preparation through exercises. The legislative basis for the interventions of the police are the radiation protection law and the upon based interventions regulation, furthermore the penal law and the law on the transport of dangerous goods. The fire brigade has federal fire fighting laws and internal regulations as a regulatory basis. Exercises of first responder organisations take place once a year at least; the scenarios reflect the actions intended by the regulations. Aeroradiometry is a special technique conducted by the police, while the fire brigade may bring heavy equipment to use. Further improvement of the cooperation of different first responder organisations is a major goal of combined exercises. (orig.)

  18. Temporal Feature Integration for Music Organisation

    DEFF Research Database (Denmark)

    Meng, Anders

    2006-01-01

    This Ph.D. thesis focuses on temporal feature integration for music organisation. Temporal feature integration is the process of combining all the feature vectors of a given time-frame into a single new feature vector in order to capture relevant information in the frame. Several existing methods...... for handling sequences of features are formulated in the temporal feature integration framework. Two datasets for music genre classification have been considered as valid test-beds for music organisation. Human evaluations of these, have been obtained to access the subjectivity on the datasets. Temporal...... ranking' approach is proposed for ranking the short-time features at larger time-scales according to their discriminative power in a music genre classification task. The multivariate AR (MAR) model has been proposed for temporal feature integration. It effectively models local dynamical structure...

  19. The organisation of work and innovative performance

    DEFF Research Database (Denmark)

    Arundel, Anthony; Lorenz, Edward; Lundvall, Bengt-Åke;

    that are used to explore at the level of national innovation systems the relation between innovation and the organisation of work. In order to construct these aggregate measures we make use of micro data from two European surveys: the third European survey of Working Conditions and the third Community......It is widely recognised that while expenditures on research and development are important inputs to successful innovation, these are not the only inputs. Further, rather than viewing innovation as a linear process, recent work on innovation in business and economics literatures characterises...... Innovation Survey (CIS-3). Although our data can only show correlations rather than causality they support the view that how firms innovate is linked to the way work is organised to promote learning and problem-solving....

  20. Organisational Learning: Positioning Selves and Creating Meaning

    DEFF Research Database (Denmark)

    Elkjær, Bente

    In this paper, I report from a study of an organisation in the midst of a major restructuring from being a family oriented business to becoming a global player.      This seemed an excellent site to explore how practices may change in organisations. At first I was only able to hear two well...... were told from specific perspectives on the current restructurings as well as on the history and the anticipated future of the enterprise. While I regard the ‘for' and ‘against' positioning as closing towards further inquiry, I propose that it may be through the work with the third ‘both-and' position...

  1. Financing and organisation of veterinary services.

    Science.gov (United States)

    Gallacher, M; Barcos, L

    2012-08-01

    This paper analyses the different ways of financing official Veterinary Services (VS) and the effects of these choices on the performance of such Services. The links between governance, organisational effectiveness and financing arrangements are seen as particularly important. The paper comments on some of the advantages and disadvantages of financing VS with service fees, as compared to budget transfers from general government revenues. Evidence is presented on the considerable heterogeneity in the size of VS and on the impact of this heterogeneity on organisation and financing. The paper concludes with a stylised case study, which emphasises the importance of collaboration and the division of labour between the official and the private sector of the veterinary profession.

  2. The IRAS project organisation and mission operations

    Science.gov (United States)

    Van Holtz, R. C.

    1983-01-01

    The project organisation of IRAS is described, showing the tasks assigned to each project group during post-launch operations. The satellite is described, emphasizing the detectors. In the task division, the role of the U.S. is to construct the telescope and survey instrument, launch the satellite, process final science data for the survey instrument, and provide certain standard satellite items. The Netherlands construct the spacecraft and three additional instruments, integrates and tests the overall satellite, and designs and participates in the development of the operational system. The U.K. provides the operational control center and primary tracking station, generates a system for preliminary science analysis of the survey data, provides housekeeping analysis software and science data distribution software, and staffs the control center operations. The teams involved in mission planning and operations, and their roles, are identified, and a block diagram of the operations organisation is presented.

  3. APPROACHES TO EMPLOYEE DEVELOPMENT IN CZECH ORGANISATIONS

    Directory of Open Access Journals (Sweden)

    VNOUČKOVÁ, Lucie

    2015-03-01

    Full Text Available Education, development, knowledge management, career development and talent management are currently often discussed themes regarding strategic management of organisations. Those concepts are strategically important. Therefore the aim of the article is to evaluate possibilities of employee education and development and identify main approaches to employee development in Czech organizations. The results are based on a quantitative survey by questionnaire data collection. The results shows that 70% of respondents have possibility of development; 86% uses their skills and abilities and 63% stated that their employer support their development. On the contrary, 27% do not feel any possibility to grow and that may lead to disaffection, loss of production or even to employee turnover. Based on the results of the analysis, employees, who miss adequate level of development are usually key and knowledge employees; it is necessary to support their career plans and development to retain them in organisation.

  4. Collaboration with East African security organisations

    DEFF Research Database (Denmark)

    Nordby, Johannes Riber; Jacobsen, Katja L.

    2012-01-01

    of the concept. At the same time the three organisations represent different constellations of member nations and thus different national interests, and locally they have different legitimacy and political strength. Thus, when choosing collaboration partners for a security project it is not simply a question...... of looking at these organisations’ military capabilities. There are other concerns, such as their aims, their internal relations, regional legitimacy and so on, to take into consideration. In this DIIS Policy Brief the authors sketch some of the differences that should be taken into consideration when...... planning to work with these organisations. The brief is the first outcome of a research project that analyses the regional security actors in East Africa and how Denmark can contribute to the regional security through them....

  5. Organisational culture: A contemporary management perspective

    Directory of Open Access Journals (Sweden)

    Richard Weeks

    2010-12-01

    Full Text Available Purpose: The purpose of this paper is to analyse the concept "organisational culture from a traditional and a more contemporary management perspective. Problem investigated: The concept organizational culture has assumed a very prominent place within the management literature and has been analysed from diverse multidisciplinary perspectives. Central to the importance attributed to the concept is the fact that it acts as a perceptual and behavioural determinant, which implies that it will have a significant impact on all human related institutional activities and thus the interest in the concept and its management. Researchers and management practitioners have come to assume that while the concept is difficult to manage in practice, it is possible to determine the prevailing culture of an institution, identify what is termed to be a desired culture for an institution and then analyse the cultural gap that exists. Based on the analysis conducted, it is further frequently assumed that the transition from the prevailing to a desired culture can be managed. A more contemporary management perspective, based on complexity theory, would appear to challenge the assumption of being able to actively manage the organisation's culture transformation process to ensure that a desired culture is manifest within the institution. The traditional and more contemporary approaches for dealing with the concept "organisational culture" are the focus of discussion and analysis in this paper. Methodology: A multidisciplinary literature review and analysis is undertaken to gain an insight of traditional and contemporary management theory and practice, as it relates to the concept "organisational culture" and its management. Findings: An important conclusion drawn from the study is that traditional paradigms of organisational culture management, that evolved within a more mechanistic manufacturing economy, is no longer effective for dealing with the unpredictable and

  6. Designing the organisational chart from the bottom

    DEFF Research Database (Denmark)

    Giangreco, Antonio; Carugati, Andrea

    This is the first of a two-case series (408-026-1 and 408-027-1). Marco Ginola was hired as the Human Resources (HR) Director of a large municipality in central Italy. The organisation had gone through a phase of major expansion which left problems in co-ordination, integration, delegation...... and control. Marco had been called in because of his reputation for being an effective innovator with unconventional ideas for the public sector. Previously, during his long career in the civil service, Marco proved to be an effective leader and negotiator who was open to other people's view points. He would...... share any significant and final decisions with his employees, rather than merely imposing his own personal choice. After spending some time in the organisation, he put into action a bottom up method to redesign the structure of the HR department. He decided to temporarily suspend the existing internal...

  7. The network organisation of consumer complaints

    CERN Document Server

    Rocha, Luis Enrique Correa; 10.1209/0295-5075/91/28005

    2010-01-01

    Interaction between consumers and companies can create conflict. When a consensus is unreachable there are legal authorities to resolve the case. This letter is a study of data from the Brazilian Department of Justice from which we build a bipartite network of categories of complaints linked to the companies receiving those complaints. We find the complaint categories organised in an hierarchical way where companies only get complaints of lower degree if they already got complaints of higher degree. The fraction of resolved complaints for a company appears to be nearly independent on the equity of the company but is positively correlated with the total number of complaints received. We construct feature vectors based on the edge-weight - the weight of an edge represents the times complaints of a category have been filed against that company - and use these vectors to study the similarity between the categories of complaints. From this analysis, we obtain trees mapping the hierarchical organisation of the comp...

  8. Organisational models for agricultural co-operatives

    OpenAIRE

    Gunnarsson Myrelid, Patrik

    1999-01-01

    The aim of the thesis is to study the effects that different financial solutions may have on the organisational mode of Irish dairy co-operatives. Membership, marketing and management are examined in four different enterprises. These areas are examined using the agency theory and property rights theory. Glanbia Group PLC, Golden Vale PLC and Kerry Group PLC were all former traditional co-operatives transformed into Public Limited Companies (PLCs) in the 1980s. Dairygold remained a traditional...

  9. Conceptual and Theoretical Frameworks for Organised Violence

    OpenAIRE

    Shaw, Martin

    2009-01-01

    The possibility of violence is ubiquitous in human social relations; its forms are manifold and its causes complex. Different types of violence are inter- related, but in complex ways, and they are studied within a wide range of disciplines, so that a general theory, while possible, is difficult to achieve. This paper, acknowledging that violence can negate power and that all forms of social power can entail violence, proceeds on the assumption that the organisation of violence is a particula...

  10. Developing organisational vision in general practice.

    OpenAIRE

    al-Shehri, A; Stanley, I; Thomas, P

    1993-01-01

    Vision is a fashionable but ill defined term in management circles. Nevertheless, it embodies a significant concept related to guiding an organisation from present realities, through opportunities and hazards, to a viable future. Until recently a typical general practice could assume a stable external environment, but now it is caught up in the uncertainties stemming from the NHS reforms. For such a practice to undertake effective strategic planning it will have to develop a vision connecting...

  11. Industrial Organisation of the Chinese Food Economy

    OpenAIRE

    Ahmadi-Esfahani, Fredoun Z.; Locke, Christopher G.

    1996-01-01

    The controversy on the convergence of socialism and capitalism has recently been focused on China. In this paper, an industrial organisation framework is used to portray the key features of "Socialism with Chinese Characteristics." The structure of supply and demand and the process of price formation is analysed to explicate the ramifications of the Chinese reform process, and the changing patterns of both government intervention and market forces in the Chinese food economy. An examination o...

  12. Bronchiolitis obliterans organising pneumonia simulating bronchial carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, J.; Flower, C. [Department of Radiology, Addenbrooke`s Hospital, University of Cambridge Teaching Hospital (United Kingdom); Schnyder, P. [Department of Radiology, University Hospital, Lausanne (Switzerland); Herold, C. [Department of Radiology, University Hospital of Vienna (Austria)

    1998-09-01

    Idiopathic bronchiolitis obliterans organising pneumonia (BOOP) is an uncommon but well-recognised condition that usually presents radiologically as bilateral multifocal patchy areas of consolidation on the chest radiograph and on computed tomography (CT). Five cases are described in which the presenting feature was that of a solitary pulmonary nodule. Four of these nodules showed evidence of cavitation and three patients presented with haemoptysis. In all cases the appearances closely resembled bronchial carcinoma. (orig.) With 5 figs., 21 refs.

  13. The institutional dynamics of voluntary organisations

    OpenAIRE

    Aagaard, Peter

    2008-01-01

    What features of institutional change do voluntary organisations contain? This question is debated in the civil society literature, but often under different headlines, like social entrepreneurship or social movement theory. The question of voluntarism is often not taken into account. This paper builds upon the premise that institutional dynamic is connected to peoples ability to act according to their free will.  But only in the ideal version are they able to make a complete connection betwe...

  14. Organisational Culture and the Family Business

    OpenAIRE

    Adiguna, Rocky

    2015-01-01

    The paper reviews the literature on organisational culture in family business by tracing its origin in organization studies and its importation to family business field. Despite the vast literature in the last three decades, cultural inquiries in family business have not come a long way. To date, research on family business culture are heavily dominated by functionalist/integrationist perspective that assumes culture as coherent and shared values. The paper suggests future research to examine...

  15. Die Organisation der Zahnarztpraxis aus Patientensicht

    OpenAIRE

    Platen, Claudia

    2001-01-01

    The dental care is in a phase of radical change. Not only the proper medical treatment but also the the organisation of the dentist's office has become a major interest for the patients. On the one hand this doctor thesis describes the reality of dentist's officeorganisation and on the other hand the requests of the patients concerning possible changes. Based on a questionnaire investigation, 434 persons betweeen 20 and 30 years from 75 different towns in NRW were interviewed selected by chan...

  16. Studies in Organisational Semiotics: an introduction

    OpenAIRE

    Rodney Clarke

    2001-01-01

    The broad application of semiotic approaches to organisations has been considered by a number of information systems academics to be a necessary advance in information systems theory (see for examples Land 1985, Rzevski 1985, and Tully 1985). Along with psychology and sociology, semiotics is considered to be a foundation discipline for information systems within the IFIP WG 8.1 FRISCO Framework (Falkenberg, et al eds/ 2000). Semiotics examines the processes of production and consumption of me...

  17. Hierarchical organisation in perception of orientation.

    Science.gov (United States)

    Spinelli, D; Antonucci, G; Daini, R; Martelli, M L; Zoccolotti, P

    1999-01-01

    According to Rock [1990, in The Legacy of Solomon Asch (Hillsdale, NJ: Lawrence Erlbaum Associates)], hierarchical organisation of perception describes cases in which the orientation of an object is affected by the immediately surrounding elements in the visual field. Various experiments were performed to study the hierarchical organisation of orientation perception. In most of them the rod-and-frame-illusion (RFI: change of the apparent vertical measured on a central rod surrounded by a tilted frame) was measured in the presence/absence of a second inner frame. The first three experiments showed that, when the inner frame is vertical, the direction and size of the illusion are consistent with expectancies based on the hierarchical organisation hypothesis. An analysis of published and unpublished data collected on a large number of subjects showed that orientational hierarchical effects are independent from the absolute size of the RFI. In experiments 4 to 7 we examined the perceptual conditions of the inner stimulus (enclosure, orientation, and presence of luminance borders) critical for obtaining a hierarchical organisation effect. Although an inner vertical square was effective in reducing the illusion (experiment 3), an inner circle enclosing the rod was ineffective (experiment 4). This indicates that definite orientation is necessary to modulate the illusion. However, orientational information provided by a vertical or horizontal rectangle presented near the rod, but not enclosing it, did not modulate the RFI (experiment 5). This suggests that the presence of a figure with oriented contours enclosing the rod is critical. In experiments 6 and 7 we studied whether the presence of luminance borders is important or whether the inner upright square might be effective also if made of subjective contours. When the subjective contour figure was salient and the observers perceived it clearly, its effectiveness in modulating the RFI was comparable to that observed with

  18. Organisational culture: A contemporary management perspective

    OpenAIRE

    Richard Weeks

    2010-01-01

    Purpose: The purpose of this paper is to analyse the concept "organisational culture from a traditional and a more contemporary management perspective. Problem investigated: The concept organizational culture has assumed a very prominent place within the management literature and has been analysed from diverse multidisciplinary perspectives. Central to the importance attributed to the concept is the fact that it acts as a perceptual and behavioural determinant, which implies that it wi...

  19. Temporal Feature Integration for Music Organisation

    OpenAIRE

    Meng, Anders; Larsen, Jan; Hansen, Lars Kai

    2006-01-01

    This Ph.D. thesis focuses on temporal feature integration for music organisation. Temporal feature integration is the process of combining all the feature vectors of a given time-frame into a single new feature vector in order to capture relevant information in the frame. Several existing methods for handling sequences of features are formulated in the temporal feature integration framework. Two datasets for music genre classification have been considered as valid test-beds for music organisa...

  20. Organisational standards for the delivery of intensity-modulated radiation therapy in Ontario.

    Science.gov (United States)

    Whitton, A; Warde, P; Sharpe, M; Oliver, T K; Bak, K; Leszczynski, K; Etheridge, S; Fleming, K; Gutierrez, E; Favell, L; Green, E

    2009-04-01

    By minimising the effect of irradiation on surrounding tissue, intensity-modulated radiation therapy (IMRT) can deliver higher, more effective doses to the targeted tumour site, minimising treatment-related morbidity and possibly improving cancer control and cure. A multidisciplinary IMRT Expert Panel was convened to develop the organisational standards for the delivery of IMRT. The systematic literature search used MEDLINE, EMBASE, the Cochrane Database, the National Guidelines Clearing House and the Health Technology Assessment Database. An environmental scan of unpublished literature used the Google search engine to review the websites of key organisations, cancer agencies/centres and vendor sites in Canada, the USA, Australia and Europe. In total, 22 relevant guidance documents were identified; 12 from the published literature and 10 from the environmental scan. Professional and organisational standards for the provision of IMRT were developed through the analysis of this evidence and the consensus opinion of the IMRT Expert Panel. The resulting standards address the following domains: planning of new IMRT programmes, practice setting requirements, tools, devices and equipment requirements; professional training requirements; role of personnel; and requirements for quality assurance and safety. Here the IMRT Expert Panel offers organisational and professional standards for the delivery of IMRT, with the intent of promoting innovation, improving access and enhancing patient care.

  1. Conceptual and Theoretical Frameworks for Organised Violence

    Directory of Open Access Journals (Sweden)

    Martin Shaw

    2009-05-01

    Full Text Available The possibility of violence is ubiquitous in human social relations; its forms are manifold and its causes complex. Different types of violence are inter- related, but in complex ways, and they are studied within a wide range of disciplines, so that a general theory, while possible, is difficult to achieve. This paper, acknowledging that violence can negate power and that all forms of social power can entail violence, proceeds on the assumption that the organisation of violence is a particular source of social power. It therefore explores the general relationships of violence to power, the significance of war as the archetype of organised violence, the relationships of other types (revolution, terrorism, genocide to war, and the significance of civilian-combatant stratification for the understanding of all types of organised violence. It then discusses the problems of applying conceptual types in analysis and the necessity of a historical framework for theorising violence. The paper concludes by offering such a framework in the transition from industrialised total war to global surveillance war.

  2. Organisational challenges of maintenance work at NPPs

    International Nuclear Information System (INIS)

    The proper working of the machinery is critical to NPP safety and productivity. Because maintenance routines and plant modifications are the activities that intervene most with the machinery, they are also the dominant sources of faults. Most of the human factor studies have relied on this fact. Due to the diversity, the temporal and spatial separation of the maintenance tasks, and the numerous competence requirements, focusing on a single task, special situation or a single psychological problem can only partially explain the requirements of maintenance work and the organisational challenges of effective maintenance. We have applied a cultural approach to maintenance work. Our aim has been to model the maintenance task and its psychological requirements and to characterise the features of organisational cultures in three NPP maintenance units. Results imply similarities and differences in the cultures and in the emphasis on the maintenance task. Maintenance activities have been under various restructuring initiatives. These changes have been experienced as stressful among the personnel. The effect of changes on the reliability of maintenance should be considered. A challenge for maintenance is to be able to build organisational structures and practices that would facilitate the fulfilment of the psychological work characteristics. (orig.)

  3. Analysing Business Interaction in a Virtual Organisation: Using Business Action Theory to Study Complex Inter-Organisational Contexts

    OpenAIRE

    Karin Axelsson

    2003-01-01

    This paper focuses upon the complexity of inter-organisational systems (IOS) — on one hand enabling organisations to become successful actors conducting electronic commerce, and on the other hand being organisationally very complex and demanding to develop, implement, and use, since several organisations with differing objectives are involved. The paper proposes a model to analyse business interaction: the business action theory (BAT) phase model. The aim of the paper is to show how BAT can...

  4. Conditional Mutations in the Mitotic Chromosome Binding Function of the Bovine Papillomavirus Type 1 E2 Protein

    OpenAIRE

    Zheng, Peng-Sheng; Brokaw, Jane; Alison A McBride

    2005-01-01

    The papillomavirus E2 protein is required for viral transcriptional regulation, DNA replication and genome segregation. We have previously shown that the E2 transactivator protein and BPV1 genomes are associated with mitotic chromosomes; E2 links the genomes to cellular chromosomes to ensure efficient segregation to daughter nuclei. The transactivation domain of the E2 protein is necessary and sufficient for association of the E2 protein with mitotic chromosomes. To determine which residues o...

  5. X chromosome inactivation: Activation of Silencing

    NARCIS (Netherlands)

    I.H. Jonkers (Iris)

    2009-01-01

    textabstractX chromosome inactivation is a process that ensures equal expression of the X chromosomes between males, which have one X and one Y chromosome, and females, which have two X chromosomes, in mammals. Females initiate inactivation of one of their two X chromosomes early during embryogenesi

  6. Chromosome Connections: Compelling Clues to Common Ancestry

    Science.gov (United States)

    Flammer, Larry

    2013-01-01

    Students compare banding patterns on hominid chromosomes and see striking evidence of their common ancestry. To test this, human chromosome no. 2 is matched with two shorter chimpanzee chromosomes, leading to the hypothesis that human chromosome 2 resulted from the fusion of the two shorter chromosomes. Students test that hypothesis by looking for…

  7. X-chromosome workshop.

    Science.gov (United States)

    Paterson, A D

    1998-01-01

    Researchers presented results of ongoing research to the X-chromosome workshop of the Fifth World Congress on Psychiatric Genetics, covering a wide range of disorders: X-linked infantile spasms; a complex phenotype associated with deletions of Xp11; male homosexuality; degree of handedness; bipolar affective disorder; schizophrenia; childhood onset psychosis; and autism. This report summarizes the presentations, as well as reviewing previous studies. The focus of this report is on linkage findings for schizophrenia and bipolar disorder from a number of groups. For schizophrenia, low positive lod scores were obtained for markers DXS991 and DXS993 from two studies, although the sharing of alleles was greatest from brother-brother pairs in one study, and sister-sister in the other. Data from the Irish schizophrenia study was also submitted, with no strong evidence for linkage on the X chromosome. For bipolar disease, following the report of a Finnish family linked to Xq24-q27, the Columbia group reported some positive results for this region from 57 families, however, another group found no evidence for linkage to this region. Of interest, is the clustering of low positive linkage results that point to regions for possible further study. PMID:9686435

  8. Epigenetic Histone Marks of Extended Meta-Polycentric Centromeres of Lathyrus and Pisum Chromosomes.

    Science.gov (United States)

    Neumann, Pavel; Schubert, Veit; Fuková, Iva; Manning, Jasper E; Houben, Andreas; Macas, Jiří

    2016-01-01

    Species of the legume genera Lathyrus and Pisum possess chromosomes that exhibit a unique structure of their centromeric regions, which is clearly apparent during metaphase by the formation of extended primary constrictions which span up to a third of the length of the chromosome. In addition, these species express two different variants of the CenH3 protein which are co-localized in multiple domains along the poleward surface of the primary constrictions. Here, we show that the constrictions represent a distinct type of chromatin differing from the chromosome arms. In metaphase, histone phosphorylation patterns including H3S10ph, H3S28ph, and H3T3ph were observed along the entire constriction, in a way similar to holocentric chromosomes. On the other hand, distribution of phosphorylated H2AT120 was different from that previously reported from either, holocentric and monocentric chromosomes, occurring at chromatin surrounding but not overlapping CenH3 domains. Since some of these phosphorylations play a role in chromatid cohesion, it can be assumed that they facilitate correct chromosome segregation by ensuring that multiple separate CenH3 domains present on the same chromatid are oriented toward the same pole. The constrictions also displayed distinct patterns of histone methylation marks, being enriched in H3K9me2 and depleted in H3K4me3 and H3K27me2 compared to the chromosome arms. Super-resolution fluorescence microscopy revealed that although both CenH3 protein variants are present in all CenH3 domains detected on metaphase chromosomes, they are only partially co-localized while there are chromatin subdomains which are mostly made of only one CenH3 variant. Taken together, these data revealed specific features of extended primary constrictions of Lathyrus and Pisum and support the idea that they may represent an intermediate stage between monocentric and holocentric chromosomes. PMID:26973677

  9. Epigenetic histone marks of extended meta-polycentric centromeres of Lathyrus and Pisum chromosomes

    Directory of Open Access Journals (Sweden)

    Pavel eNeumann

    2016-03-01

    Full Text Available Species of the legume genera Lathyrus and Pisum possess chromosomes that exhibit a unique structure of their centromeric regions, which is clearly apparent during metaphase by the formation of extended primary constrictions which span up to a third of the length of the chromosome. In addition, these species express two different variants of the CenH3 protein which are co-localized in multiple domains along the poleward surface of the primary constrictions. Here we show that the constrictions represent a distinct type of chromatin differing from the chromosome arms. In metaphase, histone phosphorylation patterns including H3S10ph, H3S28ph and H3T3ph were observed along the entire constriction, in a way similar to holocentric chromosomes. On the other hand, distribution of phosphorylated H2AT120 was different from that previously reported from either, holocentric and monocentric chromosomes, occurring at chromatin surrounding but not overlapping CenH3 domains. Since some of these phosphorylations play a role in chromatid cohesion, it can be assumed that they facilitate correct chromosome segregation by ensuring that multiple separate CenH3 domains present on the same chromatid are oriented towards the same pole. The constrictions also displayed distinct patterns of histone methylation marks, being enriched in H3K9me2 and depleted in H3K4me3 and H3K27me2 compared to the chromosome arms. High resolution fluorescence microscopy revealed that although both CenH3 protein variants are present in all CenH3 domains detected on metaphase chromosomes, they are only partially co-localized while there are chromatin subdomains which are mostly made of only one CenH3 variant. Taken together, these data revealed specific features of extended primary constrictions of Lathyrus and Pisum and support the idea that they may represent an intermediate stage between monocentric and holocentric chromosomes.

  10. Materiality and the Enduring Aspects of Organisational Identities

    DEFF Research Database (Denmark)

    Backer, Lise

    2007-01-01

    This article contributes to the emerging body of work in organisational theory that seeks to include materiality in conceptualising processes of organizing (e.g. Law, 1994; Doolin, 2003; Czarniawska and Gustavsson, 2004; Dale, 2005). Using the four largest multinational oil companies' green...... transition towards renewable energies as a case the article integrates material aspects into the theory on the narration of organisational identities. Following Czarniawska (1997) the concept of organisational identity is viewed as an evolving organisational narrative. Following Law (1994....... The article concludes to that end that both social and material aspects add to the endurance of organisational identities....

  11. At the confluence of organisation development (OD and organisation identity theory (OIT: Enter identity interventions

    Directory of Open Access Journals (Sweden)

    C L Van Tonder

    2004-10-01

    Full Text Available The identity concept has been around in the form of “corporate identity" for some time, but its appearance as “organisation identity" is more recent. Emerging theory and initial empirical research suggest that an identity approach and “identity interventions" in particular, offer promising avenues to the organisation development practitioner for enhancing organisational focus, building resilience in the face of major change, and improving performance. Identity interventions in and of themselves, but also employed as pre-change interventions, build organisational capacity that would stave off premature organisational “death" and extend the organisation’s life expectancy. Opsomming Die identiteitskonsep is in die vorm van korporatiewe identiteit reeds ’n geruime tyd in omgang, maar die verskyning daarvan as “organisasie-identiteit? is meer onlangs. Ontluikende teorie en aanvanklike empiriese navorsing suggereer dat ’n identiteitsbenadering en "identiteitsintervensies" in die besonder, belowende geleenthede aan die organisasie- ontwikkelingspraktisyn bied om organisasiefokus te verbeter, die organisasie se veerkragtigheid ten aanskoue van omvangryke verandering te bou, en prestasie te verbeter. Identiteitsintervensies op sigself bou organisasiekapasiteit, maar kan ook as voorveranderingsintervensies aangewend word wat premature organisasie "sterftes" sal vermy en die organisasie se lewensverwagting sal verleng.

  12. Learning to Adapt. Organisational Adaptation to Climate Change Impacts

    International Nuclear Information System (INIS)

    Analysis of human adaptation to climate change should be based on realistic models of adaptive behaviour at the level of organisations and individuals. The paper sets out a framework for analysing adaptation to the direct and indirect impacts of climate change in business organisations with new evidence presented from empirical research into adaptation in nine case-study companies. It argues that adaptation to climate change has many similarities with processes of organisational learning. The paper suggests that business organisations face a number of obstacles in learning how to adapt to climate change impacts, especially in relation to the weakness and ambiguity of signals about climate change and the uncertainty about benefits flowing from adaptation measures. Organisations rarely adapt 'autonomously', since their adaptive behaviour is influenced by policy and market conditions, and draws on resources external to the organisation. The paper identifies four adaptation strategies that pattern organisational adaptive behaviour

  13. Organisational Culture and Corporate Performance: Empirical Evidence from Nigeria

    Directory of Open Access Journals (Sweden)

    Olu Ojo

    2014-09-01

    Full Text Available The purpose of this research article is to examine various concepts on organisational culture and strives to ascertain the importance of the relationship between organisational culture and corporate performance in a business context. The study adopted survey research design. The population of this study is the entire employees of Nigerian commercial banks. Primary data were used for this study. Data were collected through a questionnaire that was administered to the selected respondents, The two hypotheses proffered were tested and relevant recommendations were made. The conclusion drawn from the study is that organisational culture plays a vital role in an organisation’s general performance. This study contributes to organisational culture’s literature by showing that employees would commit themselves to organisational goals and work actively in achieving those goals when they buy into cultural norms of the organisation and thus increase organisational performance.

  14. Causes of oncogenic chromosomal translocation

    OpenAIRE

    Aplan, Peter D.

    2005-01-01

    Non-random chromosomal translocations are frequently associated with a variety of cancers, especially hematologic malignancies and childhood sarcomas In addition to their diagnostic utility, chromosomal translocations are increasingly being used in the clinic to guide therapeutic decisions. However, the mechanisms which cause these translocations remain poorly understood. Illegit...

  15. Cohesin in determining chromosome architecture

    Energy Technology Data Exchange (ETDEWEB)

    Haering, Christian H., E-mail: christian.haering@embl.de [Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg (Germany); Jessberger, Rolf, E-mail: rolf.jessberger@tu-dresden.de [Institute of Physiological Chemistry, Dresden University of Technology, Dresden (Germany)

    2012-07-15

    Cells use ring-like structured protein complexes for various tasks in DNA dynamics. The tripartite cohesin ring is particularly suited to determine chromosome architecture, for it is large and dynamic, may acquire different forms, and is involved in several distinct nuclear processes. This review focuses on cohesin's role in structuring chromosomes during mitotic and meiotic cell divisions and during interphase.

  16. On the equivalence between kernel self-organising maps and self-organising mixture density networks.

    Science.gov (United States)

    Yin, Hujun

    2006-01-01

    The kernel method has become a useful trick and has been widely applied to various learning models to extend their nonlinear approximation and classification capabilities. Such extensions have also recently occurred to the Self-Organising Map (SOM). In this paper, two recently proposed kernel SOMs are reviewed, together with their link to an energy function. The Self-Organising Mixture Network is an extension of the SOM for mixture density modelling. This paper shows that with an isotropic, density-type kernel function, the kernel SOM is equivalent to a homoscedastic Self-Organising Mixture Network, an entropy-based density estimator. This revelation on the one hand explains that kernelising SOM can improve classification performance by acquiring better probability models of the data; but on the other hand it also explains that the SOM already naturally approximates the kernel method.

  17. Genetics Home Reference: ring chromosome 20 syndrome

    Science.gov (United States)

    ... 3 links) Encyclopedia: Chromosome Encyclopedia: Epilepsy Health Topic: Epilepsy Genetic and Rare Diseases Information Center (1 link) Ring chromosome 20 Additional NIH Resources (2 links) National Human Genome Research Institute: Chromosome Abnormalities National Institute of ...

  18. Genetics Home Reference: ring chromosome 14 syndrome

    Science.gov (United States)

    ... Encyclopedia: Chromosome Health Topic: Developmental Disabilities Health Topic: Epilepsy Genetic and Rare Diseases Information Center (1 link) Ring chromosome 14 Additional NIH Resources (2 links) National Human Genome Research Institute: Chromosome Abnormalities National Institute of ...

  19. Bacterial chromosome organization and segregation.

    Science.gov (United States)

    Badrinarayanan, Anjana; Le, Tung B K; Laub, Michael T

    2015-01-01

    If fully stretched out, a typical bacterial chromosome would be nearly 1 mm long, approximately 1,000 times the length of a cell. Not only must cells massively compact their genetic material, but they must also organize their DNA in a manner that is compatible with a range of cellular processes, including DNA replication, DNA repair, homologous recombination, and horizontal gene transfer. Recent work, driven in part by technological advances, has begun to reveal the general principles of chromosome organization in bacteria. Here, drawing on studies of many different organisms, we review the emerging picture of how bacterial chromosomes are structured at multiple length scales, highlighting the functions of various DNA-binding proteins and the impact of physical forces. Additionally, we discuss the spatial dynamics of chromosomes, particularly during their segregation to daughter cells. Although there has been tremendous progress, we also highlight gaps that remain in understanding chromosome organization and segregation. PMID:26566111

  20. Higher order structure of chromosomes.

    Science.gov (United States)

    Okada, T A; Comings, D E

    1979-04-01

    Isolated Chinese hamster metaphase chromosomes were resuspended in 4 M ammonium acetate and spread on a surface of distilled water or 0.15 to 0.5 M ammonium acetate. The DNA was released in the form of a regular series of rosettes connected by interrossette DNA. The mean length of the rosette DNA was 14 micron, similar to the mean length of 10 micron for chromomere DNA of Drosophila polytene chromosomes. The mean interrosette DNA was 4.2 micron. SDS gel electrophoresis of the chromosomal nonhistone proteins showed them to be very similar to nuclear nonhistone proteins except for the presence of more actin and tubulin. Nuclear matrix proteins were present in the chromosomes and may play a role in forming the rosettes. Evidence that the rosette pattern is artifactual versus the possibility that it represents a real organizational substructure of the chromosomes is reviewed.

  1. ADN et chromosomes

    OpenAIRE

    Hayes, Hélène

    2000-01-01

    Chaque chromosome contient une seule molécule d’ADN. L’ADN déroulé d’un noyau de cellule humaine mesurerait environ 1,8 m : chaque molécule d’ADN est enroulée et compactée en plusieurs étapes, grâce à l’association de différentes protéines, et loge dans le noyau de 6 µm de diamètre. Le degré de condensation de l’ADN est variable selon les régions chromosomiques et les régions les moins condensées sont les plus riches en gènes. L’ADN est composé d’une variété de séquences codantes ou non et ré...

  2. Schizophrenia and chromosomal deletions

    Energy Technology Data Exchange (ETDEWEB)

    Lindsay, E.A.; Baldini, A. [Baylor College of Medicine, Houston, TX (United States); Morris, M. A. [Univ. of Geneva School of Medicine, NY (United States)] [and others

    1995-06-01

    Recent genetic linkage analysis studies have suggested the presence of a schizophrenia locus on the chromosomal region 22q11-q13. Schizophrenia has also been frequently observed in patients affected with velo-cardio-facial syndrome (VCFS), a disorder frequently associated with deletions within 22q11.1. It has been hypothesized that psychosis in VCFS may be due to deletion of the catechol-o-methyl transferase gene. Prompted by these observations, we screened for 22q11 deletions in a population of 100 schizophrenics selected from the Maryland Epidemiological Sample. Our results show that there are schizophrenic patients carrying a deletion of 22q11.1 and a mild VCFS phenotype that might remain unrecognized. These findings should encourage a search for a schizophrenia-susceptibility gene within the deleted region and alert those in clinical practice to the possible presence of a mild VCFS phenotype associated with schizophrenia. 9 refs.

  3. X-Chromosome dosage compensation.

    Science.gov (United States)

    Meyer, Barbara J

    2005-01-01

    In mammals, flies, and worms, sex is determined by distinctive regulatory mechanisms that cause males (XO or XY) and females (XX) to differ in their dose of X chromosomes. In each species, an essential X chromosome-wide process called dosage compensation ensures that somatic cells of either sex express equal levels of X-linked gene products. The strategies used to achieve dosage compensation are diverse, but in all cases, specialized complexes are targeted specifically to the X chromosome(s) of only one sex to regulate transcript levels. In C. elegans, this sex-specific targeting of the dosage compensation complex (DCC) is controlled by the same developmental signal that establishes sex, the ratio of X chromosomes to sets of autosomes (X:A signal). Molecular components of this chromosome counting process have been defined. Following a common step of regulation, sex determination and dosage compensation are controlled by distinct genetic pathways. C. elegans dosage compensation is implemented by a protein complex that binds both X chromosomes of hermaphrodites to reduce transcript levels by one-half. The dosage compensation complex resembles the conserved 13S condensin complex required for both mitotic and meiotic chromosome resolution and condensation, implying the recruitment of ancient proteins to the new task of regulating gene expression. Within each C. elegans somatic cell, one of the DCC components also participates in the separate mitotic/meiotic condensin complex. Other DCC components play pivotal roles in regulating the number and distribution of crossovers during meiosis. The strategy by which C. elegans X chromosomes attract the condensin-like DCC is known. Small, well-dispersed X-recognition elements act as entry sites to recruit the dosage compensation complex and to nucleate spreading of the complex to X regions that lack recruitment sites. In this manner, a repressed chromatin state is spread in cis over short or long distances, thus establishing the

  4. A role for chromatin topology in imprinted domain regulation.

    Science.gov (United States)

    MacDonald, William A; Sachani, Saqib S; White, Carlee R; Mann, Mellissa R W

    2016-02-01

    Recently, many advancements in genome-wide chromatin topology and nuclear architecture have unveiled the complex and hidden world of the nucleus, where chromatin is organized into discrete neighbourhoods with coordinated gene expression. This includes the active and inactive X chromosomes. Using X chromosome inactivation as a working model, we utilized publicly available datasets together with a literature review to gain insight into topologically associated domains, lamin-associated domains, nucleolar-associating domains, scaffold/matrix attachment regions, and nucleoporin-associated chromatin and their role in regulating monoallelic expression. Furthermore, we comprehensively review for the first time the role of chromatin topology and nuclear architecture in the regulation of genomic imprinting. We propose that chromatin topology and nuclear architecture are important regulatory mechanisms for directing gene expression within imprinted domains. Furthermore, we predict that dynamic changes in chromatin topology and nuclear architecture play roles in tissue-specific imprint domain regulation during early development and differentiation.

  5. Chromatid Painting for Chromosomal Inversion Detection Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose the continued development of a novel approach to the detection of chromosomal inversions. Transmissible chromosome aberrations (translocations and...

  6. Chromatid Painting for Chromosomal Inversion Detection Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose a novel approach to the detection of chromosomal inversions. Transmissible chromosome aberrations (translocations and inversions) have profound genetic...

  7. Achieving excellence in private intensive care units: The effect of transformational leadership and organisational culture on organisational change outcomes

    Directory of Open Access Journals (Sweden)

    Portia J. Jordan

    2015-03-01

    Full Text Available Orientation: Organisational change outcomes in private intensive care units are linked to higher patient satisfaction, improved quality of patient care, family support, cost-effective care practices and an increased level of excellence. Transformational leadership and fostering a positive organisational culture can contribute to these change outcomes.Research purpose: The study determined whether transformational leadership and a supportive organisational culture were evident in six private intensive care units in the Eastern Cape, South Africa. A conceptual framework to investigate the relationship between transformational leadership, organisational culture, and organisational change outcomes, was proposed and tested.Motivation for the study: The prevalence of transformational leadership, a positive organisational culture and their effect on organisational change outcomes in private healthcare industries require further research in order to generate appropriate recommendations.Research design, approach and method: A positivistic, quantitative design was used. A survey was conducted using a questionnaire which, in previous studies, produced scores with Cronbach’s alpha coefficients greater than 0.80, to collect data from a sample of 130 professional nurses in private intensive care units.Main findings: Transformational leadership and a positive organisational culture were evident in the private intensive care units sampled. A strong, positive correlation exists between transformational leadership, organisational culture, and organisational change outcomes. This correlation provides sufficient evidence to accept the postulated research hypotheses. Innovation and intellectual stimulation were identified as the factors in need of improvement.Practical or managerial implications: The findings of the study may be used by managers in intensive care units to promote organisational change outcomes, linked to transformational leadership and a positive

  8. Organisational learning: Between organizing and knowing

    DEFF Research Database (Denmark)

    Elkjær, Bente; Brandi, Ulrik; Nielsen, Steffen Bohni

    This paper explores how management consultants share knowledge amongst different countries and offices through an empirical study of management consultancy. We propose that an understanding of knowledge sharing across countries and offices is to be combined with theories from the field...... of organisational learning. The paper show that knowledge sharing is just as much about processes ‘around’ work (e.g. the interactions in a project group) than the actual knowledge ‘about’ work indicating that knowledge sharing amongst management consultants is situated and negotiated rather than fixed...

  9. POT: Planning, Organisation and Tele-Information

    DEFF Research Database (Denmark)

    Vidal, Rene Victor Valqui

    1996-01-01

    The main purpose of this paper is to introduce the framwork of a research project carried out at the Technical University of Denmark dealing with 'holistic' and interdisciplinary (systems oriented)methods for strategic planning and organisational re-design in connection with the introduction...... of tele-information technologies and hte new demands of hte so-called information society, the POT proejct. Three specirfic projects are shortly introduced: planning in networked firms, strategic approaches to climate change and strategies for the schools of the future....

  10. Typehusbranchens organisation, produktion og marked og innovation

    DEFF Research Database (Denmark)

    Haugbølle, Kim; Forman, Marianne

    Denne dokumentationsrapport beskriver resultaterne af et survey af typehusbranchens organisering, produktion, marked og innovation. På trods af eller måske netop på grund af den aktuelle finanskrise vil en fortsat udvikling af typehusbyggeriet og det industrialiserede byggeri generelt kræve en øget...... har producenterne? - Hvem er bygherrerne? - Hvilke typer af innovation finder sted? - Hvordan forløber innovationsprocessen? Undersøgelsen yder således et bidrag til en forståelse af byggeriets processer og innovation indenfor et område, som er relativt upåagtet i både dansk og international...

  11. Organisational design for an integrated oncological department

    Directory of Open Access Journals (Sweden)

    Ch.L. Meiss-de Haas

    2001-09-01

    Full Text Available Objective: The outcomes of a Strength, Weakness, Opportunities and Threat (SWOT analysis of three Integrated Oncological Departments were compared with their present situation three years later to define factors that can influence a successful implementation and development of an Integrated Oncological Department in- and outside (i.e. home care the hospital. Research design: Comparative Qualitative Case Study. Methods: Auditing based on care-as-usual norms by an external, experienced auditing committee. Research setting: Integrated Oncological Departments of three hospitals. Results: Successful multidisciplinary care in an integrated, oncological department needs broad support inside the hospital and a well-defined organisational plan.

  12. Mitotic chromosome condensation in vertebrates

    Energy Technology Data Exchange (ETDEWEB)

    Vagnarelli, Paola, E-mail: P.Vagnarelli@ed.ac.uk

    2012-07-15

    Work from several laboratories over the past 10-15 years has revealed that, within the interphase nucleus, chromosomes are organized into spatially distinct territories [T. Cremer, C. Cremer, Chromosome territories, nuclear architecture and gene regulation in mammalian cells, Nat. Rev. Genet. 2 (2001) 292-301 and T. Cremer, M. Cremer, S. Dietzel, S. Muller, I. Solovei, S. Fakan, Chromosome territories-a functional nuclear landscape, Curr. Opin. Cell Biol. 18 (2006) 307-316]. The overall compaction level and intranuclear location varies as a function of gene density for both entire chromosomes [J.A. Croft, J.M. Bridger, S. Boyle, P. Perry, P. Teague,W.A. Bickmore, Differences in the localization and morphology of chromosomes in the human nucleus, J. Cell Biol. 145 (1999) 1119-1131] and specific chromosomal regions [N.L. Mahy, P.E. Perry, S. Gilchrist, R.A. Baldock, W.A. Bickmore, Spatial organization of active and inactive genes and noncoding DNA within chromosome territories, J. Cell Biol. 157 (2002) 579-589] (Fig. 1A, A'). In prophase, when cyclin B activity reaches a high threshold, chromosome condensation occurs followed by Nuclear Envelope Breakdown (NEB) [1]. At this point vertebrate chromosomes appear as compact structures harboring an attachment point for the spindle microtubules physically recognizable as a primary constriction where the two sister chromatids are held together. The transition from an unshaped interphase chromosome to the highly structured mitotic chromosome (compare Figs. 1A and B) has fascinated researchers for several decades now; however a definite picture of how this process is achieved and regulated is not yet in our hands and it will require more investigation to comprehend the complete process. From a biochemical point of view a vertebrate mitotic chromosomes is composed of DNA, histone proteins (60%) and non-histone proteins (40%) [6]. I will discuss below what is known to date on the contribution of these two different classes

  13. Intermingling of chromosome territories in interphase suggests role in translocations and transcription-dependent associations.

    Directory of Open Access Journals (Sweden)

    Miguel R Branco

    2006-05-01

    Full Text Available After mitosis, mammalian chromosomes partially decondense to occupy distinct territories in the cell nucleus. Current models propose that territories are separated by an interchromatin domain, rich in soluble nuclear machinery, where only rare interchromosomal interactions can occur via extended chromatin loops. In contrast, recent evidence for chromatin mobility and high frequency of chromosome translocations are consistent with significant levels of chromosome intermingling, with important consequences for genome function and stability. Here we use a novel high-resolution in situ hybridization procedure that preserves chromatin nanostructure to show that chromosome territories intermingle significantly in the nucleus of human cells. The degree of intermingling between specific chromosome pairs in human lymphocytes correlates with the frequency of chromosome translocations in the same cell type, implying that double-strand breaks formed within areas of intermingling are more likely to participate in interchromosomal rearrangements. The presence of transcription factories in regions of intermingling and the effect of transcription impairment on the interactions between chromosomes shows that transcription-dependent interchromosomal associations shape chromosome organization in mammalian cells. These findings suggest that local chromatin conformation and gene transcription influence the extent with which chromosomes interact and affect their overall properties, with direct consequences for cell-type specific genome stability.

  14. Shape Transitions and Chiral Symmetry Breaking in the Energy Landscape of the Mitotic Chromosome.

    Science.gov (United States)

    Zhang, Bin; Wolynes, Peter G

    2016-06-17

    We derive an unbiased information theoretic energy landscape for chromosomes at metaphase using a maximum entropy approach that accurately reproduces the details of the experimentally measured pairwise contact probabilities between genomic loci. Dynamical simulations using this landscape lead to cylindrical, helically twisted structures reflecting liquid crystalline order. These structures are similar to those arising from a generic ideal homogenized chromosome energy landscape. The helical twist can be either right or left handed so chiral symmetry is broken spontaneously. The ideal chromosome landscape when augmented by interactions like those leading to topologically associating domain formation in the interphase chromosome reproduces these behaviors. The phase diagram of this landscape shows that the helical fiber order and the cylindrical shape persist at temperatures above the onset of chiral symmetry breaking, which is limited by the topologically associating domain interaction strength.

  15. Shape Transitions and Chiral Symmetry Breaking in the Energy Landscape of the Mitotic Chromosome

    Science.gov (United States)

    Zhang, Bin; Wolynes, Peter G.

    2016-06-01

    We derive an unbiased information theoretic energy landscape for chromosomes at metaphase using a maximum entropy approach that accurately reproduces the details of the experimentally measured pairwise contact probabilities between genomic loci. Dynamical simulations using this landscape lead to cylindrical, helically twisted structures reflecting liquid crystalline order. These structures are similar to those arising from a generic ideal homogenized chromosome energy landscape. The helical twist can be either right or left handed so chiral symmetry is broken spontaneously. The ideal chromosome landscape when augmented by interactions like those leading to topologically associating domain formation in the interphase chromosome reproduces these behaviors. The phase diagram of this landscape shows that the helical fiber order and the cylindrical shape persist at temperatures above the onset of chiral symmetry breaking, which is limited by the topologically associating domain interaction strength.

  16. Barriers of inter-organisational integration in vocational rehabilitation

    Directory of Open Access Journals (Sweden)

    Ulla Wihlman

    2008-06-01

    Full Text Available Introduction: A project of vocational rehabilitation was studied in Sweden between 1999 and 2002. The project included four public organisations: the social insurance office, the local health services, the municipal social service and the office of the state employment service. The aim of this paper was to analyse perceived barriers in the development of inter-organisational integration. Theory: Theories of inter-professional and inter-organisational integration, and theories on organisational change. Methods: In total, 51 semi-structured interviews and 14 focus group discussions were performed with actors within the project between 1999 and 2002. A thematic approach was used for the analysis of the data. Results: Three different main themes of barriers emerged from the data: A Uncertainty, B Prioritising own organisation and C Lack of communication. The themes are interconnected in an intricate web and hence not mutually exclusive. Conclusions and discussion: The barriers found are all related partly to organisational change in general and partly to the specific development of organisational integration. Prioritising of own organisation led to flaws in communication, which in turn led to a high degree of uncertainty within the project. This can be seen as a circular relationship, since uncertainty might increase focus on own organisation and lack of communication. A way to overcome these barriers would be to take the needs of the clients as a point of departure in the development of joint services and to also involve them in the development of inter-organisational integration.

  17. Organisational reviews - requirements, methods and experience. Progress report 2006

    International Nuclear Information System (INIS)

    Organisational reviews are important instruments in the continuous quest for improved performance. In the nuclear field there has been an increasing regulatory interest in organisational performance, because incidents and accidents often point to organisational deficiencies as one of the major precursors. Many methods for organisational reviews have been proposed, but they are mostly based on ad hoc approaches to specific problems. The absence of well-established techniques for organisational reviews has already shown to cause discussions and controversies on different levels. The aim of the OrRe project is to collect the experiences from organisational reviews carried out so far and to reflect them in a theoretical model of organisational performance. Furthermore, the project aims to reflect on the criteria for the definition of the scope and content of organisational reviews. Finally, recommendations will be made for guidance for people participating in organisational reviews. This progress report describes regulatory practices in Finland and Sweden together with some case examples of organizational reviews and assessment in both countries. Some issues of concern are raised and an outline for the next year's work is proposed. Issues of concern include the sufficient depth of the assessment, the required competence in assessments, data and criteria problems, definition of the boundaries of the system to be assessed, and the necessary internal support and organisational maturity required for successful assessments. Finally, plans for next year's work are outlined. (au)

  18. Organisational reviews - requirements, methods and experience. Progress report 2006

    Energy Technology Data Exchange (ETDEWEB)

    Reiman, T.; Oedewald, P.; Wahlstroem, B. [VTT, Technical Research Centre of Finland (Finland); Rollenhagen, C.; Kahlbom, U. [Maelardalen University (FI)

    2007-04-15

    Organisational reviews are important instruments in the continuous quest for improved performance. In the nuclear field there has been an increasing regulatory interest in organisational performance, because incidents and accidents often point to organisational deficiencies as one of the major precursors. Many methods for organisational reviews have been proposed, but they are mostly based on ad hoc approaches to specific problems. The absence of well-established techniques for organisational reviews has already shown to cause discussions and controversies on different levels. The aim of the OrRe project is to collect the experiences from organisational reviews carried out so far and to reflect them in a theoretical model of organisational performance. Furthermore, the project aims to reflect on the criteria for the definition of the scope and content of organisational reviews. Finally, recommendations will be made for guidance for people participating in organisational reviews. This progress report describes regulatory practices in Finland and Sweden together with some case examples of organizational reviews and assessment in both countries. Some issues of concern are raised and an outline for the next year's work is proposed. Issues of concern include the sufficient depth of the assessment, the required competence in assessments, data and criteria problems, definition of the boundaries of the system to be assessed, and the necessary internal support and organisational maturity required for successful assessments. Finally, plans for next year's work are outlined. (au)

  19. What matters for organisational change? Evidence from DEPZ, Bangladesh

    Directory of Open Access Journals (Sweden)

    Md. Zohurul Islam

    2013-01-01

    Full Text Available Orientation: The role of leadership and human resources (HRM at the managerial level in the economic zones to implement organisational change have been well described in developing countries although they are often not well documented.Research purpose: The purpose of this article is to investigate the relationship between leadership, organisational behaviour and HRM in Dhaka export processing zone (DEPZ enterprises.Motivation for the study: This study has given a direction for implementing organisational change in DEPZ organisations, where leadership, organisational behaviour and HRM have significant effects on organisational change.Research design, approach and method: The author completed a survey using a structured questionnaire on 53 enterprises in the DEPZ. The sample size was 216. The author tested the research hypotheses by using statistical tools like step-wise multiple regression analysis. The author also used Pearson correlations, a t-test, an ANOVA and a radar diagram in this study.Main findings: The results provide evidence that leadership behaviour, organisational behaviour factors and HRM practices have direct relationships with organisational change. In short, it requires high level of leadership ability, employee motivation and commitment, recruitment, performance appraisal and reward to bring about effective organisational change.Practical/managerial implications: The results show that organisational learning, transformational and transactional leadership, compensation and unionisation practices reinforce organisational change at DEPZ enterprises.Contribution/value-add: The results of this study show that organisational change requires integration with leadership ability, organisational behaviour and HRM practices, which are useful for developing companies, industries and the national economy.

  20. Gametocidal chromosomes enhancing chromosome aberration in common wheat induced by 5-azacytidine.

    Science.gov (United States)

    Su, W-Y; Cong, W-W; Shu, Y-J; Wang, D; Xu, G-H; Guo, C-H

    2013-01-01

    The gametocidal (Gc) chromosome from Aegilops spp induces chromosome mutation, which is introduced into common wheat as a tool of chromosome manipulation for genetic improvement. The Gc chromosome functions similar to a restriction-modification system in bacteria, in which DNA methylation is an important regulator. We treated root tips of wheat carrying Gc chromosomes with the hypomethylation agent 5-azacytidine; chromosome breakage and micronuclei were observed in these root tips. The frequency of aberrations differed in wheat containing different Gc chromosomes, suggesting different functions inducing chromosome breakage. Gc chromosome 3C caused the greatest degree of chromosome aberration, while Gc chromosome 3C(SAT) and 2C caused only slight chromosome aberration. Gc chromosome 3C induced different degrees of chromosome aberration in wheat varieties Triticum aestivum var. Chinese Spring and Norin 26, demonstrating an inhibition function in common wheat. PMID:23884766

  1. Challenging the Post-Fordist/Flexible Organisation Thesis: The Case of Reformed Educational Organisations

    Science.gov (United States)

    Brehony, Kevin J.; Deem, Rosemary

    2005-01-01

    This paper examines claims that recent reforms to UK education have led to significant organisational changes in primary school and higher education. It also examines two main theoretical explanations for these, namely post-Fordism and New Managerialism. Examples of changes in both schools and universities, including flexibility and teamwork, are…

  2. European ways to combat psychosocial risks related to work organisation : towards organisational interventions?

    NARCIS (Netherlands)

    Oeij, P.R.A.; Morvan, E.; Houtman, I.L.D.; Vaas, F.; Wiezer, N.

    2004-01-01

    From 24-26 November 2004, the 6h Annual Conference of the European Academy of Occupational Health Psychology ‘Healthy, Efficient & Productive Organisations’ was held in Oporto, Portugal. During this conference, the Workshop ‘Organisational interventions to combat psychosocial factors of stress’ was

  3. Entropy-driven spatial organization of highly confined polymers: Lessons for the bacterial chromosome

    Science.gov (United States)

    Jun, Suckjoon; Mulder, Bela

    2006-08-01

    Despite recent progress in visualization experiments, the mechanism underlying chromosome segregation in bacteria still remains elusive. Here we address a basic physical issue associated with bacterial chromosome segregation, namely the spatial organization of highly confined, self-avoiding polymers (of nontrivial topology) in a rod-shaped cell-like geometry. Through computer simulations, we present evidence that, under strong confinement conditions, topologically distinct domains of a polymer complex effectively repel each other to maximize their conformational entropy, suggesting that duplicated circular chromosomes could partition spontaneously. This mechanism not only is able to account for the spatial separation per se but also captures the major features of the spatiotemporal organization of the duplicating chromosomes observed in Escherichia coli and Caulobacter crescentus. bacterial chromosome segregation | Caulobacter crescentus | Escherichia coli | polymer physics

  4. The network organisation of consumer complaints

    Science.gov (United States)

    Rocha, L. E. C.; Holme, P.

    2010-07-01

    Interaction between consumers and companies can create conflict. When a consensus is unreachable there are legal authorities to resolve the case. This letter is a study of data from the Brazilian Department of Justice from which we build a bipartite network of categories of complaints linked to the companies receiving those complaints. We find the complaint categories organised in an hierarchical way where companies only get complaints of lower degree if they already got complaints of higher degree. The fraction of resolved complaints for a company appears to be nearly independent of the equity of the company but is positively correlated with the total number of complaints received. We construct feature vectors based on the edge-weight —the weight of an edge represents the times complaints of a category have been filed against that company— and use these vectors to study the similarity between the categories of complaints. From this analysis, we obtain trees mapping the hierarchical organisation of the complaints. We also apply principal component analysis to the set of feature vectors concluding that a reduction of the dimensionality of these from 8827 to 27 gives an optimal hierarchical representation.

  5. Incorporating organisational safety culture within ergonomics practice.

    Science.gov (United States)

    Bentley, Tim; Tappin, David

    2010-10-01

    This paper conceptualises organisational safety culture and considers its relevance to ergonomics practice. Issues discussed in the paper include the modest contribution that ergonomists and ergonomics as a discipline have made to this burgeoning field of study and the significance of safety culture to a systems approach. The relevance of safety culture to ergonomics work with regard to the analysis, design, implementation and evaluation process, and implications for participatory ergonomics approaches, are also discussed. A potential user-friendly, qualitative approach to assessing safety culture as part of ergonomics work is presented, based on a recently published conceptual framework that recognises the dynamic and multi-dimensional nature of safety culture. The paper concludes by considering the use of such an approach, where an understanding of different aspects of safety culture within an organisation is seen as important to the success of ergonomics projects. STATEMENT OF RELEVANCE: The relevance of safety culture to ergonomics practice is a key focus of this paper, including its relationship with the systems approach, participatory ergonomics and the ergonomics analysis, design, implementation and evaluation process. An approach to assessing safety culture as part of ergonomics work is presented.

  6. Numerous transitions of sex chromosomes in Diptera.

    Directory of Open Access Journals (Sweden)

    Beatriz Vicoso

    2015-04-01

    Full Text Available Many species groups, including mammals and many insects, determine sex using heteromorphic sex chromosomes. Diptera flies, which include the model Drosophila melanogaster, generally have XY sex chromosomes and a conserved karyotype consisting of six chromosomal arms (five large rods and a small dot, but superficially similar karyotypes may conceal the true extent of sex chromosome variation. Here, we use whole-genome analysis in 37 fly species belonging to 22 different families of Diptera and uncover tremendous hidden diversity in sex chromosome karyotypes among flies. We identify over a dozen different sex chromosome configurations, and the small dot chromosome is repeatedly used as the sex chromosome, which presumably reflects the ancestral karyotype of higher Diptera. However, we identify species with undifferentiated sex chromosomes, others in which a different chromosome replaced the dot as a sex chromosome or in which up to three chromosomal elements became incorporated into the sex chromosomes, and others yet with female heterogamety (ZW sex chromosomes. Transcriptome analysis shows that dosage compensation has evolved multiple times in flies, consistently through up-regulation of the single X in males. However, X chromosomes generally show a deficiency of genes with male-biased expression, possibly reflecting sex-specific selective pressures. These species thus provide a rich resource to study sex chromosome biology in a comparative manner and show that similar selective forces have shaped the unique evolution of sex chromosomes in diverse fly taxa.

  7. Familial transmission of a deletion of chromosome 21 derived from a translocation between chromosome 21 and an inverted chromosome 22.

    Science.gov (United States)

    Aviv, H; Lieber, C; Yenamandra, A; Desposito, F

    1997-06-27

    Chromosome analysis of a newborn boy with Down syndrome resulted in the identification of a family with an unusual derivative chromosome 22. The child has 46 chromosomes, including two chromosomes 21, one normal chromosome 22, and a derivative chromosome 22. Giemsa banding and fluorescent in situ hybridization (FISH) studies show that the derivative chromosome is chromosome 22 with evidence of both paracentric and pericentric inversions, joined to the long arm of chromosome 21 from 21q21.2 to qter. The rearrangement results in partial trisomy 21 extending from 21q21.2 to 21q terminus in the patient. The child's mother, brother, maternal aunt, and maternal grandmother are all carriers of the derivative chromosome. All have 45 chromosomes, with one normal chromosome 21, one normal chromosome 22, and the derivative chromosome 22. The rearrangement results in the absence of the short arm, the centromere, and the proximal long arm of chromosome 21 (del 21pter-21q21.2) in carriers. Carriers of the derivative chromosome in this family have normal physical appearance, mild learning disabilities and poor social adjustment. PMID:9182781

  8. Towards an organisation-wide process-oriented organisation of care: A literature review

    Directory of Open Access Journals (Sweden)

    Groenewegen Peter P

    2011-01-01

    Full Text Available Abstract Background Many hospitals have taken actions to make care delivery for specific patient groups more process-oriented, but struggle with the question how to deal with process orientation at hospital level. The aim of this study is to report and discuss the experiences of hospitals with implementing process-oriented organisation designs in order to derive lessons for future transitions and research. Methods A literature review of English language articles on organisation-wide process-oriented redesigns, published between January 1998 and May 2009, was performed. Results Of 329 abstracts identified, 10 articles were included in the study. These articles described process-oriented redesigns of five hospitals. Four hospitals tried to become process-oriented by the implementation of coordination measures, and one by organisational restructuring. The adoption of the coordination mechanism approach was particularly constrained by the functional structure of hospitals. Other factors that hampered the redesigns in general were the limited applicability of and unfamiliarity with process improvement techniques. Conclusions Due to the limitations of the evidence, it is not known which approach, implementation of coordination measures or organisational restructuring (with additional coordination measures, produces the best results in which situation. Therefore, more research is needed. For this research, the use of qualitative methods in addition to quantitative measures is recommended to contribute to a better understanding of preconditions and contingencies for an effective application of approaches to become process-oriented. Hospitals are advised to take the factors for failure described into account and to take suitable actions to counteract these obstacles on their way to become process-oriented organisations.

  9. Digital Ecosystems: Self-Organisation of Evolving Agent Populations

    CERN Document Server

    Briscoe, Gerard

    2008-01-01

    A primary motivation for research in digital ecosystems is the desire to exploit the self-organising properties of natural ecosystems, because they are thought to be robust, scalable architectures that can automatically solve complex, dynamic problems. Self-organisation is perhaps one of the most desirable features in the systems that we design, and it is important for us to be able to measure such self-organising behaviour. We investigate the self-organising aspects of Digital Ecosystems, created by the application of evolutionary computing to Multi-Agent Systems aiming to determine a macroscopic variable to characterise the self-organisation of the evolving agent populations within our Digital Ecosystem. We study a measure for self-organisation called Physical Complexity, which is based on statistical physics, automata theory, and information theory. It provides a measure of the quantity of information in an organism's genome, relative to the environment in which it evolves, by calculating the entropy in th...

  10. A Review of IT Service Management and Organisational Changes

    DEFF Research Database (Denmark)

    Kræmmergaard, Pernille; Lynge, Bjarne Christoffer; Schou, Christoffer Dalby

    The interest in Information technology service management (ITSM) is increasing in practice and also in research we will argue. The aim of implementation of ITSM in IT organisations is to increase the service towards the IT organisations´ customers, businesses and users, and that requires changes...... in the way many IT organisations operate and organize themselves around IT. We set out to identify the organisational changes in relation to the implementation of ITSM in organisational setting using Leavitt's diamond model and a literature study in more than 100 IS journals. One of the findings reported...... here is that not much has been published on organisational changes associated with the implementation of ITSM. Thus more research in this area is needed, and it confirms the argument that ITSM is a growing field of interest....

  11. Organisational culture: an important concept for pharmacy practice research.

    Science.gov (United States)

    Scahill, Shane; Harrison, Jeff; Carswell, Peter; Babar, Zaheer-Ud-Din

    2009-10-01

    Throughout the developed world, community pharmacy is under considerable pressure to play a greater part in delivering effective primary health care. The requirement to adopt new roles continues to challenge community pharmacy and drive change. The factors that determine the ability of community pharmacy to effectively deliver services for health gain are complex and include; policy, professional, financial and structural elements. There is also evidence to suggest that organisational culture may influence the effectiveness of an organisation. In order to address this there is a need to understand the dimensions of organisational culture that lead to successful implementation of the change necessary for community pharmacy to become a more effective primary health care organisation. In this commentary, we introduce the concept of organisational culture, outline two frameworks for studying culture, and argue the benefits of pursuing an organisational culture research agenda for the evolution of pharmacy practice and research.

  12. Organising Ethics: The Case of the Norwegian Army

    Directory of Open Access Journals (Sweden)

    Ellen-Marie Forsberg

    2012-05-01

    Full Text Available This article shows how institutionalism, a theory in organisational social science, provides a model for diagnosing organisational challenges that influence the ethical practices and integration in the Norwegian Army. Institutionalism provides tools for analysing the differences between expressed values and actual practices and for understanding the organisational dynamics that unfold at the crossroads of the organisation's formal structure, informal culture and stakeholder relations. In this article we present and discuss such differences and dynamics in the Norwegian Army based on findings from a survey and a number of workshops. We also provide some suggestions for effective implementation of strategies for strengthening ethics in such an organisation. We argue that the perspective taken in this project is also relevant for other highly professionalised complex organisations and that such interdisciplinary research will strengthen practical ethics' potential for real impact.http://dx.doi.org/10.5324/eip.v6i1.1779

  13. Promoting institutional and organisational development in surveying and land administration

    DEFF Research Database (Denmark)

    Enemark, Stig; Greenway, Iain

    2006-01-01

    A key component of capacity building is ensuring that a country’s organisations are sufficiently robust to develop, enable and ensure the effective operation of surveying and land administration activities. The relevant organisations include the professional surveying associations (the FIG member...... associations), the private surveying companies, as well as the government agencies such as the mapping organisations and the organisations with land registration and land administration responsibilities. This paper provides a conceptual understanding covering the area of institutional and organisational...... and actions); How do we stay there (sustainability). The paper proposes the establishment of an FIG Task Force that brings together expertise to support countries in this area of institutional and organisational development. The Task Force would create support mechanisms and material for countries...

  14. Meiosis and chromosome painting of sex chromosome systems in Ceboidea.

    Science.gov (United States)

    Mudry, M D; Rahn, I M; Solari, A J

    2001-06-01

    The identity of the chromosomes involved in the multiple sex system of Alouatta caraya (Aca) and the possible distribution of this system among other Ceboidea were investigated by chromosome painting of mitotic cells from five species and by analysis of meiosis at pachytene in two species. The identity of the autosome #7 (X2) involved in the multiple system of Aca and its breakage points were demonstrated by both meiosis and chromosome painting. These features are identical to those described by Consigliere et al. [1996] in Alouatta seniculus sara (Assa) and Alouatta seniculus arctoidea (Asar). This multiple system was absent in the other four Ceboidea species studied here. However, data from the literature strongly suggest the presence of this multiple in other members of this genus. The presence of this multiple system among several species and subspecies that show high levels of chromosome rearrangements may suggest a special selective value of this multiple. The meiotic features of the sex systems of Aca and Cebus apella paraguayanus (Cap) are strikingly different at pachytene, as the latter system is similar to the sex pair of man and other primates. The relatively large genetic distances between species presently showing this multiple system suggest that its origin is not recent. Other members of the same genus should be investigated at meiosis and by chromosome painting in order to know the extent and distribution of this complex sex-chromosome system. PMID:11376445

  15. Preparing organisations for employee-driven open innovation

    OpenAIRE

    Amundsen, O.; Aasen, T.M.; Gressgard, L.J.

    2014-01-01

    The present study addresses the need to prepare organisations, small or large, for open innovation approaches, including the development of capacity to exploit the potential benefits of such principles through Employee-Driven Innovation (EDI). Based on interviews in 20 Norwegian enterprises, we propose that EDI is an under-explored opportunity in many organisations, and that the systematic introduction of EDI practices increases organisations' ability to exploit open innovation principles and...

  16. Intention based modelling of organisational change: an experience report

    OpenAIRE

    Rolland, Colette; Loucopoulos, Pericles; Kavakli, Vagelio; Nurcan, Selmin

    1999-01-01

    The traditional approach to information systems development has proved to be too monolithic for dealing with highly complex, multidimensional, organisational change issues. In the traditional paradigm, little attempt is made in understanding how the proposed system relates to other organisational components or the effect that the system will have on the enterprise itself. This lack of knowledge ramifies throughout the system development process, making it difficult to identify organisational ...

  17. Variability in forms of organisation in biotechnology firms

    OpenAIRE

    Luukkonen, Terttu

    2003-01-01

    This paper examines variability in forms of organisation, in terms of forward and backward networking versus vertical integration, in biotechnology SMEs. The study examines forms of organisation in a set of firms across different application segments. The forms of organisation vary by application segment in biotechnology, but differences are not clear-cut, and a firm can apply different forms to different application segments in its activities. Reasons for the variability are related to the s...

  18. Innovation and the Organisation of Technical Expertise and Work

    OpenAIRE

    Howelss, John

    2004-01-01

    This paper reviews a selection of the comparative research on the organisation of skills and work in different countries. It argues that despite differences in institutional means, such as keiretsu and structured apprenticeship, the ends, in terms of a capacity to organise skills, is similar. It is suggested that the British institution of craft control of skills should be understood as the default state of organisation of skills and work. This state may be likely to develop anywhere where th...

  19. DO INNOVATIVE ORGANISATIONS COMPETE ON SINGLE OR MULTIPLE OPERATIONAL CAPABILITIES?

    OpenAIRE

    ALKA ASHWINI NAND; PRAKASH J. SINGH; ANANYA BHATTACHARYA

    2014-01-01

    Organisations lack clear guidance on how they can become more innovative at the operational level. The operations strategy literature shows that organisations compete on four generic capabilities: cost efficiency, quality of products or services, speed of delivery, and flexibility of operations. Should organisations choose between these capabilities, i.e., engage in trading-off these capabilities and focussing on one capability ("trade-off" model), or combine them, thereby competing on multip...

  20. Balancing Agility and Discipline in a Medical Device Software Organisation

    OpenAIRE

    Mc Hugh, Martin; McCaffery, Fergal; Fitzgerald, Brian; Stol, Klass-Jan; COADY, Garret; Casey, Valentine

    2013-01-01

    peer-reviewed Agile development techniques are becoming increasingly popular in the generic software development industry as they appear to offer solutions to the problems associated with following a plan-driven Software Development Life Cycle (SDLC). However, agile methods may not be suited to all industries or organisations. For agile methods to succeed, an organisation must be structured in a way to accommodate agile methods. Medical device software development organisations are bound b...