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Sample records for chromatography methodenentwicklung zu

  1. Chromatography.

    Science.gov (United States)

    Brantley, L. Reed, Sr.; Demanche, Edna L.; Klemm, E. Barbara; Kyselka, Will; Phillips, Edwin A.; Pottenger, Francis M.; Yamamoto, Karen N.; Young, Donald B.

    This booklet presents some activities on chromatography. Directions for preparing leaf pigment extracts using alcohol are given, and paper chromatography and thin-layer chromatography are described as modifications of the basic principles of chromatography. (KHR)

  2. Zwei Anmerkungen zu WACC

    OpenAIRE

    Löffler, Andreas

    2017-01-01

    Der WACC–Ansatz zählt zu den beliebtesten Verfahren der Unternehmensbewertung. In dieser Note wird gezeigt, dass dieser Ansatz auch dann verwendet werden kann, wenn die von Miles und Ezzell getroffene Annahme einer konstanten Kapitalstruktur fallen gelassen und die WACC-Bewertungsgleichung entsprechend angepasst wird. Des Weiteren wird gezeigt, wie der WACC-Ansatz in ein zeitstetiges Modell übertragen werden kann. Die sich ergebende Bewertungsgleichung zeigt große Ähnlichkeiten mit den Er...

  3. Hin zu Utopia - eine empirische Spekulation

    Directory of Open Access Journals (Sweden)

    Wolfgang Frindte

    2017-04-01

    Full Text Available Hin zu Utopia - eine empirische Spekulation (von Wolfgang Frindte & Nico Dietrich Der vorliegende Text nutzt Befunde, die die Autoren im Rahmen einer Studie zu Einstellungen gegenüber Muslimen und dem Islam erhoben haben (Frindte/Dietrich 2017, um über die Möglichkeit einer kosmopolitischen Utopie zu spekulieren und nach den Akteuren der Zukunft und den künftigen Gestaltern eines friedlichen und menschenfreundlichen europäischen Kontinents zu fahnden. Im Ergebnis ihrer Suche kommen die Autoren zu dem Schluss: Es gibt noch immer gebildete und politisch informierte Menschen, die eine positive Sicht auf ethnische Minderheiten, Flüchtlinge im Allgemeinen und Muslime im Besonderen haben, autoritäre und machtorientierte Gesellschaftsstrukturen ablehnen und sich mit Europa identifizieren. Auf ihren persönlichen Einsatz, ihre Entschlossenheit, ihren Zusammenhalt über die Zeit und ihre Zukunftsorientierung kommt es an.

  4. Literatur zu Gast im Globe

    CERN Multimedia

    2006-01-01

    Der deutsche Schriftsteller Thomas LEHR liest am 23. März 2006 aus seinem Roman '42', einem philosophischen Abenteuerroman über die Suche nach der Zeit, erzählt in einer funkelnden und souveränen Sprache. Eine Welt von vernichtender Schönheit und bizarren Schrecken wartet auf eine Besuchergruppe, die in der Nähe von Genf die unterirdischen Anlagen des Forschungszentrums für Teilchenphysik CERN besichtigt. Als sie um 12:47:42 wieder ans Tageslicht tritt, scheint ganz Europa in einen Dornröschenschlaf gefallen. Allein die 70 Besucher können sich noch bewegen. Monate und Jahre verharrt die Welt wie in einer riesigen dreidimensionalen Fotografie, bis ein wiederum schockierendes Ereignis die 'Chronifizierten' aus ihren physikalischen Spekulationen und seltsamen Lebensformen reisst. Zu dieser literarischen Veranstaltung im 'Globe of Science and Innovation' laden gemeinsam das Generalkonsulat der Bundesrepublik Deutschland in Genf und CERN ein. Vor Beginn der Lesung wird eine Führung in deutscher Sprac...

  5. Identification of hardly biodegradable residuals (sulfur- and nitrogen-containing substances) during waste water treatment, including the development of analytical methods; Identifizierung von schwer abbaubaren Reststoffen (stickstoff- und schwefelhaltigen Verbindungen) bei der Abwasserbehandlung, einschliesslich analytischer Methodenentwicklung

    Energy Technology Data Exchange (ETDEWEB)

    Moehle, E; Huber, A; Metzger, J W

    1999-07-01

    Organic residuals in sewage, which are not removed completely by waste water treatment may be relevant in environmental toxicology and may disturb drinking water treatment processes. The organic residuals must be identified before new techniques to eliminate these substances from waste water can be developed and steps can be taken to prevent them from polluting waste waters. In the research project sum parameters of sulfur- and nitrogen-containing substances in municipal waste water were determined. A new method was developed to determine the organic sulfur in compounds absorbed on activated carbon (AOS). The determination of dissolved organic nitrogen (DON) was calculated as the difference between total nitrogen and the sum of NH{sub 4}{sup +}-N, NO{sub 3}{sup -}-N and NO{sub 2}{sup -}N. The removal of organic substances from the inorganic matrix was only possible for standard solutions, but not for real samples. More than 60 substances contributing to the sum parameters could be identified with GC-MS and GC-AED, an most of them could be quantified. 30-70% of the sulfur-containing substances detected with GC-AED could be identified. With the GC-MS screening method 21 drugs or drug metabolites could be identified and partly quantified. Hydrophilic organic residuals were identified and quantified with high performance liquid chromatography coupled with UV- and fluorescence detectors and also with a mass detector (ESI-MS-MS). With the methods described only a small percentage of the sum of AOS and DON could be detected, although new materials for the solid phase enrichment and new analytical methods, such as HPLC-MS-MS were used. In order to get information about the degree of elimination (absorption or degradation) of different drugs in a municipal sewage plant, laboratory-scale tests under aerobic conditions were performed. A batch reactor containing drugs in environmentally relevant concentrations and a suspension of activated sludge was coupled online with HPLC

  6. Beiträge zu einer kognitiv ausgerichteten Lexikographie

    African Journals Online (AJOL)

    rbr

    Jahrhunderten immer wieder abgelegte Bekenntnis zu dem Satz Sprache dient dem Ausdruck von Gedanken oder einer seiner Varianten zeigt, kognitive Orien- tierung ein altes Programm mit Sprache befaßter Disziplinen ist, hat sich eine. Linguistik, die dieses Programm wirklich auf ihre Fahnen geschrieben hat, erst.

  7. Sind gesetze (noch immer zu hoch aufgehängt?

    Directory of Open Access Journals (Sweden)

    Ada Gruntar Jermol

    2015-08-01

    Full Text Available Es braucht nicht besonders betont zu werden, dass Gesetze für den fachlichen Laien eine rätselhafte undfast unverständliche Lektüre darstellen. Im vorliegenden Beitrag wird versucht, deutlich zu machen, warum - bildlich gesprochen - Gesetze zu hoch aufgehängt sind; oder mit anderen Worten: worauf die meisten Verständnisschwierigkeiten von Gesetzestexten zurückzuführen sind. Wurzelt die Unverstäindlichkeit von Gesetzen in ihrer sprachlichen Beschaffenheit (die Stilthese oder in der mangelnden Referenz auf die betreffenden Bezugssysteme (die Wissensthese? Die einleitende Diskussion sei mit einem Zitat Els Oksaars (1988, 185 eröffnet: Vor mehr als zweitausend Jahren liess der Tyrann Dionysios von Syrakus die Gesetze so hoch aufhängen, dass sie kein Bfuger lesen konnte. Hegel vergleicht in seiner Rechtsphilosophie dieses Umecht mit der Tatsache, dass das Recht seiner Gestalt nach nur denen zugänglich gemacht wird, die sich - wie er es ausdrückt - gelehrt darauflegen. Heute kannjeder Bürger, wenn er will, alle Gesetze lesen, es scheint jedoch, dass sich seit Dionysios im Prinzip nicht viel geändert hat: sind nicht Gesetze auch heute noch zu hoch aufgehängt, und zwar auf der Abstraktions- und Begriffsleiter der Sprache?

  8. Beiträge zu einer kognitiv ausgerichteten lexikographie | Figge ...

    African Journals Online (AJOL)

    Nach einer "kognitiven Wende" in der Psychologie in der zweiten Hälfte der sechziger Jahre haben auch Teile der Linguistik und der Psycholinguistik begonnen, sich kognitiv zu orientieren. Hier wird ein Modell zugrunde gelegt, nach dem sprachliche Einheiten auf Einheiten des Gedächtnisses referieren. Einheiten des ...

  9. Open Access für Forschung und Kultur: Die Staatsbibliothek zu Berlin unterstützt den offenen Zugang zu wissenschaftlichem Wissen

    OpenAIRE

    Staatsbibliothek zu Berlin

    2017-01-01

    Seit ihrer Gründung im Jahr 1661 ist die Staatsbibliothek zu Berlin (SBB) für die Öffentlichkeit zugänglich. In dieser Tradition sowie im Bewusstsein ihrer besonderen historischen Verantwortung steht die SBB zu ihrer Verpflichtung, wissenschaftliches Wissen der Gesellschaft unbeschränkt und gleichberechtigt zur Verfügung zu stellen. Als Einrichtung der Stiftung Preußischer Kulturbesitz hat sie die Berliner Erklärung über den offenen Zugang zu wissenschaftlichem Wissen unterzeichnet. Deren Zie...

  10. Open Access für Forschung und Kultur: Die Staatsbibliothek zu Berlin unterstützt den offenen Zugang zu wissenschaftlichem Wissen

    OpenAIRE

    Staatsbibliothek zu Berlin

    2016-01-01

    Seit ihrer Gründung im Jahr 1661 ist die Staatsbibliothek zu Berlin (SBB) für die Öffentlichkeit zugänglich. In dieser Tradition sowie im Bewusstsein ihrer besonderen historischen Verantwortung steht die SBB zu ihrer Verpflichtung, wissenschaftliches Wissen der Gesellschaft unbeschränkt und gleichberechtigt zur Verfügung zu stellen. Als Einrichtung der Stiftung Preußischer Kulturbesitz hat sie die Berliner Erklärung über den offenen Zugang zu wissenschaftlichem Wissen unterzeichnet. Deren Zie...

  11. Beiträge zu einer kognitiv ausgerichteten Lexikographie

    Directory of Open Access Journals (Sweden)

    Udo L. Figge

    2011-10-01

    Full Text Available

    Zusammenfassung: Nach einer "kognitiven Wende" in der Psychologie in der zweitenHälfte der sechziger Jahre haben auch Teile der Linguistik und der Psycholinguistik begonnen, sichkognitiv zu orientieren. Hier wird ein Modell zugrunde gelegt, nach dem sprachliche Einheiten aufEinheiten des Gedächtnisses referieren. Einheiten des Gedächtnisses sind zunächst Merkmale,sodann Konzepte, die sich aus Merkmalen konstituieren, und schließlich Gebilde von Merkmalen,Konzepten und Beziehungen zwischen Merkmalen. Eine solche Konzeption entspricht in denGrundzügen zeitgenössischen neuropsychologischen Erkenntnissen. Konzepte manifestieren sichin substantivischen Satzgliedern. Die Bedeutung eines Substantivs ist lexikographisch als Mengevon Merkmalen zu beschreiben, und zwar ohne Beachtung der ohnehin schwer zu rechtfertigendenUnterscheidung zwischen semantischem und enzyklopädischem Wissen. Die Bedeutungeines attributiven Adjektivs ist lexikographisch als Merkmal der Konzepte zu beschreiben, diedurch die zugehörigen Substantive ausgedrückt werden. Einem Verb liegt ein konverses Netz vonMerkmalen zugrunde; es muß lexikographisch dieser Bedeutung entsprechend beschrieben werden.Bei der Beschreibung von Substantiven ist systematisch zwischen echten Substantiven undsolchen Substantiven zu unterscheiden, die, meist als Ableitungen, eine verbale oder adjektivischeBedeutung haben. Insbesondere ist zu beachten, daß solche Substantive in Nebensatzäquivalentendie Funktion substantivischer Verbformen haben, daß sie weiterhin auch substantivische Bedeutungannehmen können und daß sie schließlich häufig Konstituenten von Stützverbgefügen sind.Die Bedeutung von Konjunktionen und von Präpositionen in Nebensatzäquivalenten kann nurnach einer exakten grammatischen Analyse verläßlich beschrieben werden. Texte werden unteranderem durch Textgliederungssignale gegliedert, die meist die Gestalt von Adverbien oder Adverbialenhaben. Diese

  12. "Un'artefice cristiano" : Studien zu Lavinia Fontana als Historienmalerin

    OpenAIRE

    Schmidt, Sandra

    2015-01-01

    Lavinia Fontana (1552-1614) war die erste Frau, die den Beruf der Malerin professionell ausübte und sich bereits zu Lebzeiten eine beeindruckende Reputation erarbeitete. In den Quellen wird sie als „Pittora singolare tra le donne” bezeichnet und in der Tat konnte sie aufgrund ihrer zahlreichen Aufträge und Erfolge von der Kunst leben und ihre Familie ernähren, was im 16. Jahrhundert außergewöhnlich war. Auch ihr Œuvre ist ungewöhnlich umfangreich. Lavinia Fontana hinterließ ein Gesamtwerk ...

  13. Kurz zum Klima: Zu viel Salz verdirbt den Boden

    OpenAIRE

    Zimmer, Markus; Lippelt, Jana; Frank, Jonas

    2012-01-01

    Der zunehmende Einsatz von Anlagen zur Bewässerung sowie mangelnde Erfahrungswerte und Unkenntnis über den richtigen Einsatz der künstlichen Bewässerung haben in den letzten Jahrzehnten zu einer massiven Zunahme der Versalzung des Bodens geführt. Durch diese Bodenversalzung werden weite Flächen für die Landwirtschaft unbrauchbar. Sie sind nur durch hohen Aufwand und Kosten wieder renaturierbar. Der Beitrag in der Reihe »Kurz zum Klima« gibt einen Überblick über die Versalzung in Anbaugebiet...

  14. Ein Paradigma, das es weiter zu konkretisieren gilt. Der Begriff der Mediatisierung bei Krotz

    Directory of Open Access Journals (Sweden)

    Hans-Dieter Kübler

    2010-01-01

    Full Text Available Rezension zu: Krotz, Friedrich. 2007. Mediatisierung: Fallstudien zum Wandel von Kommunikation. 1. Aufl. Medien - Kultur - Kommunikation. Wiesbaden: VS Verlag für Sozialwissenschaften.

  15. Die grossen Physiker und ihre Entdeckungen von den fallenden Körpern zu den Quarks

    CERN Document Server

    Segrè, Emilio

    1998-01-01

    Von Galileo Galilei bis zu Richard Feynman und Murray Gell-Mann - von den fallenden Körpern zu den Quarks: Der Physiknobelpreisträger Emilio Segre hat seine ganz persönliche Geschichte der Physik geschrieben. Er erzählt von den großen Gestalten und deren wichtigen Entdeckungen mit großer Anschaulichkeit und Lebendigkeit.

  16. Von eingebetteten Systemen zu Cyber-Physical Systems

    Science.gov (United States)

    Wedde, Rorst F.; Lehnhoff, Sebastian; Rehtanz, Christian; Krause, Olav

    Das Hauptanliegen des Papiers ist, ein Paradigma für Probleme mit neuartigen Integrationsanforderungen für Forschung und Entwicklung in verteilten eingebetteten Echtzeitsystemen zu motivieren und vorzustellen, nämlich den Begriff Cyber-Physical Systems. Bei einer in letzter Zeit stark zunehmenden Anzahl von Realzeitanwendungen können ohne die Berücksichtigung solcher Forderungen keine praktisch brauchbaren Lösungen erwartet werden. Einige Anwendungsfelder werden angesprochen. Im Einzelnen werden dann für Elektroautos, die mit erneuerbaren Energien betrieben werden sollen, einerseits die Management-, verteilte Verhandlungs- und Verteilungsprobleme der benötigten Energie in einem bottom-up Ansatz gelöst. Andererseits wird als Teil unserer Projektarbeit die Bereitstellung von Reserveenergie für den allgemeinen Bedarf durch Autobatterien vorgestellt. Es zeigt sich, dass dies effizienter und wesentlich kurzfristiger in unserem verteilten Vorgehen geschehen kann als in traditionellen Verfahren.

  17. Plasma chromatography

    International Nuclear Information System (INIS)

    Anon.

    1984-01-01

    This book examines the fundamental theory and various applications of ion mobility spectroscopy. Plasma chromatography developed from research on the diffusion and mobility of ions. Topics considered include instrument design and description (e.g., performance, spectral interpretation, sample handling, mass spectrometry), the role of ion mobility in plasma chromatography (e.g., kinetic theory of ion transport), atmospheric pressure ionization (e.g., rate equations), the characterization of isomers by plasma chromatography (e.g., molecular ion characteristics, polynuclear aromatics), plasma chromatography as a gas chromatographic detection method (e.g., qualitative analysis, continuous mobility monitoring, quantitative analysis), the analysis of toxic vapors by plasma chromatography (e.g., plasma chromatograph calibration, instrument control and data processing), the analysis of semiconductor devices and microelectronic packages by plasma chromatography/mass spectroscopy (e.g., analysis of organic surface contaminants, analysis of water in sealed electronic packages), and instrument design and automation (hardware, software)

  18. Hydroxylapatite chromatography.

    Science.gov (United States)

    Broadhurst, A V

    2001-05-01

    Hydroxylapatite (also called hydroxyapatite), a form of calcium phosphate, can be used as a matrix for the chromatography of both proteins and nucleic acids. Protocols are provided for both standard low-pressure chromatography of a protein mixture using a hydroxylapatite column prepared in the laboratory, and an HPLC method, applicable to proteins and nucleic acids, that uses a commercially available column. Alternate protocols describe column chromatography using a step gradient or batch binding and step-gradient elution.

  19. „Dichtes Verstehen“ als Schritt zu einer begegnenden Bibliothekskultur

    Directory of Open Access Journals (Sweden)

    Barbara Schellhammer

    2013-11-01

    Full Text Available Mit seiner ethnografischen Methode der dichten Beschreibung schlug der amerikanische Sozial­wissenschaftler Clifford Geertz eine völlig neue Richtung in der Kulturanthropologie ein. Diese sogenannte „interpretative Wende“ stellt auch jenseits der Ethnografie eine grosse Bereicherung dar. Überall wo Men­schen unterschiedlicher sozio-kultureller Herkunft aufeinander treffen, eignet sich die dichte Beschreibung, um kulturelle Bedeutungssysteme besser verstehen und einordnen zu können. Dies hilft nicht nur im Be­reich der qualitativen Sozialforschung, sondern auch ganz konkret im alltäglichen Leben, im privaten, wie auch im beruflichen Umgang mit fremden Erfahrungshorizonten. Denn die dichte Beschreibung sprengt vorgefertigte, „dünne“, sozio-kulturelle Typologien und Etikettierungen. Sie möchte vielmehr kulturelle Symbole auf deren tiefe Bedeutung phänomenologisch beschreiben und hermeneutisch ergründen. Diese Fähigkeit kann gerade auch im Umgang mit Kundinnen und Kunden im Bibliotheksbereich eine grosse Be­reicherung darstellen und dazu beitragen, eine positive Bibliothekskultur zu begründen. With his ethnographic approach Clifford Geertz established a new way of thinking and doing re­search in cultural anthropology. This so called “ethnographic turn” continues to be of great significance for many disciplines, not only for ethnological studies. Wherever people of different socio-cultural back­grounds meet, Geertz's method of “Thick Description” helps to understand foreign cultures, or, what Geertz calls “complex systems of meaning-making”. This is important for scientific research settings, but also for day-to-day encounters in multi-cultural societies in a globalized world. “Thin” forms of describing other­ness categorize, stereotype or come up with set explanations which will never be able to grasp the complex­ity and ever changing nature of peoples and cultures. Geertz's method seeks to (1 thoroughly

  20. Methodische Anregungen zu Literarischen Gesprächen in der Sekundarstufe

    OpenAIRE

    Spinner, Kaspar H.

    2014-01-01

    Methodische Anregungen zu Literarischen Gesprächen in der Sekundarstufe. - In: Standardorientierung im Lese- und Literaturunterricht der Sekundarstufe I / Cornelia Rosebrock ; Heike Wirthwein. - Baltmannsweiler : Schneider-Verl. Hohengehren, 2014. - S. 126-132

  1. [Memel als Brücke zu den baltischen Ländern] / Ralph Tuchtenhagen

    Index Scriptorium Estoniae

    Tuchtenhagen, Ralph, 1961-

    2014-01-01

    Arvustus: Memel als Brücke zu den baltischen Ländern. Kulturgeschichte Klaipedas vom Mittelalter bis ins 20. Jahrhundert. (Tagungsberichte der Historischen Kommission für ost- und westpreussische Landesforschung. Bd. 26)

  2. Ebenen des Verstehens: Überlegungen zu einem Verfahren zum Wurzelziehen

    Science.gov (United States)

    Winter, Martin

    Wir bemühen uns, insbesondere bei Kindern, den Lernprozess auch im Mathematikunterricht durch den Einsatz von Materialien zu unterstützen. Die Arbeitsschritte dienen dabei oft der Vorbereitung oder Herleitung von Verfahren - in der Hoffnung, dass durch die Veranschaulichung Zusammenhänge besser verstanden werden. Worin dann das Verstehen besteht, wenn im Ergebnis ein Verfahren von den Kindern erfolgreich abgearbeitet wird, ist nicht unmittelbar zu sehen.

  3. RECHTSEXTREME LINKS LIEGEN LASSEN? EINE EINSCHÄTZUNG VON SZENEAUSSTEIGERN ZU MEDIENEFFEKTEN INNERHALB DER RECHTSEXTREMEN SZENE

    Directory of Open Access Journals (Sweden)

    Katharina Neumann

    2014-12-01

    Full Text Available Die vorliegende Arbeit geht der Frage nach, welche Reaktionen durch unterschiedliche Formen der Berichterstattung über Rechtsextremismus bei den Anhängern der rechten Szene ausgelöst werden und welche Strategien diese verfolgen, um ihre mediale Selbstinszenierung zu optimieren. Das Ziel der Arbeit ist es, Ansätze für einen verantwortungsbewussten Umgang mit Themen rechtsextremen Schwerpunkts für Medienmacher zu finden. Es werden 7 Aussteiger zu ihren Erfahrungen mit Medien in der Szene befragt. Zu den wichtigsten Ergebnissen dieser Arbeit zählt die Erkenntnis, dass die rechtsextreme Führungsriege aktiv Berichterstattung in den Massenmedien rezipiert und systematisch auswertet, um auf dieser Basis ihre politischen Selbstinszenierungsstrategien zu entwickeln. Die Szene reagiert also taktisch auf Berichte in den Massenmedien und versucht diese durch Anschlusskommunikation für ihre Zwecke zu instrumentalisieren. Wichtig für die Berichterstattung über Rechtsextremismus erscheint den Probanden die Betonung der Pluralität der Szene und eine ausführliche Hintergrundberichterstattung, die im besten Falle identitätsstiftende Attribute rechtsextremer Gruppen dekonstruiert und somit deren Glaubwürdigkeit in Frage stellt.

  4. Zu einigen mittelalterlichen Amtsbezeichungen in der Toponymie Serbiens

    Directory of Open Access Journals (Sweden)

    Aleksandar Loma

    2015-12-01

    Full Text Available Die vorliegende Arbeit behandelt den Ortsnamen Šepšin, wie ein Dorf in Serbien unweit von Mladenovac, südlich von Belgrad, heißt. Nach dem Zeugnis der türkischen Verzeichnisse des 16. Jh.s, hat sich die heutige Form des Namens über Šemšin aus Šemčin entwickelt, wie sein Erstbeleg aus 1528 lautet. Vorausgesetzt, dass sich das anlautende Š- aus S- durch Assimilation vom Typ šiče < siče ‘zischt’ entwickelt hat, lässt sich die ursprügliche Namenform als *Sěmčijin wiederherstellen, wo sich um ein Possessivadjektiv zur altserbischen Amtsbezeichnung sěmьčija handelt. Nur als Hapax in der BanjskaUrkunde aus 1316. belegt, wird sie mit aksl. samъčii ‘praefectus’ gleichgesetzt, wo wahrscheinlich ein Lehnwort aus dem Protobulgarischen vorliegt. In diesem Fall liesse sich das ě der altserbischen Nebenform durch den Einfluss des urslav. Wortes *sěmь ‘Familie’ erklären. Anknüpfend wird die Frage nach der Herkunft eines anderen Ortsnamens aus demselben Gebiet, Hrčin, heute Vrčin, wieder in Betracht gezogen. Bisher unterschiedlich gedeutet, lässt sich er analogisch zu *Sěmčijin auf *Hrtčijin als Possessivbildung zur sonst unbelegten Hofamtsbezeichnung *xъrtьčii ‘für Windhunde (*xъrtъ zuständig, Hofjägermeister’ zurückführen. In beiden Fällen dürfte sich das maskuline Possessivadjektiv auf -in ursprünglich auf potok ‘Bach’ oder dol ‘Tal’ im Lehensbesitz der betreffenden Person bezogen haben.

  5. Analytische und Effektor-Studien von N-Acyl-Ethanolaminphosphaten

    OpenAIRE

    Ates, Ebru

    2011-01-01

    Bei N-Acyl-Ethanolaminphosphaten handelt es sich um eine bislang wenig untersuchte Klasse polarer Substanzen, deren Erforschung aufgrund ihrer strukturellen Analogie zu apolaren, physiologisch wirksamen N-Acyl-Ethanolaminen von Interesse ist. Zu bear-beiten waren analytische Fragestellungen, die auch synthetische Aufgaben beinhalteten, wie Methodenentwicklung und Versuche zur Erfassung von N-Acyl-Ethanolamin-phosphaten in ausgewählten Lebensmitteln sowie strukturelle Studien zur „Bioaktivität...

  6. Geschlecht zwischen Körper und Konstruktion: Aktuelle Impulse zu einer langen Debatte

    Directory of Open Access Journals (Sweden)

    Natascha Gruber

    2002-11-01

    Full Text Available Die mit dem Erscheinen von Judith Butlers Unbehagen der Geschlechter ausgelöste „Essentialismus versus Konstruktivismus“-Debatte um die Frage, wie Materialität und Körper zu denken sei, hält die Geschlechterforschung bis heute in dilemmatischer Spannung. Kann auf die Kategorie Geschlecht überhaupt verzichtet werden? Der Sammelband Verhandlungen des Geschlechts dokumentiert anhand von 15 Beiträgen aus den Bereichen Kulturwissenschaften, Philosophie, Psychoanalyse und Politik die aktuelle Entwicklung der Debatte rund um den an Butler orientierten feministischen Konstruktionsbegriff. Unter Rückgriff auf unterschiedliche Ansätze wird versucht, zwischen „Körper“ und „Diskurs“ zu vermitteln und Materialität neu zu denken.

  7. Ein Versuch, Naturverhältnisse aus feministischer Sicht zu interpretieren

    Directory of Open Access Journals (Sweden)

    Helga Purgand

    2002-07-01

    Full Text Available Mit dem vorliegenden Sammelband Geschlechterverhältnisse – Naturverhältnisse wird nach dem innovativen Beitrag der feministischen Debatte für umweltsoziologisches Denken gefragt und nach Überschneidungen, Grenzen und Zwischenräumen zwischen „Geschlecht“ und „Natur“ gesucht. In ihm wird der lobenswerte Versuch unternommen, einen Zusammenhang zwischen Geschlechter- und Naturverhältnissen herzustellen „die in der Umweltsoziologie bislang stark vernachlässigte feministische Diskussion und Geschlechterforschung einzubeziehen und als genderbezogene Perspektive zu etablieren“. (S. 9 Das Buch enthält Beiträge von 15 Autorinnen, die unterschiedliche Natur- und Geschlechtsverhältnisse facettenreich, überwiegend kreativ und theoretisch anspruchsvoll betrachten. Das Buch enthält Zugänge aus der allgemeinen Soziologie, den Sprach- und Kulturwissenschaften, aus politik- und naturwissenschaftlichen Zusammenhängen. Den ausdrücklich formulierten Anspruch, von multi- zu einer inter-disziplinären Durchdringung zu gelangen, erfüllt es nur in Ansätzen (besonders mit der Einleitung und den theoretischen Problemexplikationen. Das zustande gekommene „Nach-, Zusammen- und Querdenken“ über Begriffe, Theorien und neuere Forschungsansätze (von Foucault über Butler bis zu Donna Haraway belegt die Notwendigkeit, die naturwissenschaftlichen Annahmen „immanent zu dekonstruieren“. Sehr aufschlussreich sind in diesem Zusammenhang die Beiträge von Irmgard Schultz, Elvira Scheich, aber auch von Mary Mellor und besonders von Paula-Irene Villa, die interessante und kreative Gedanken zur Klärung des Verhältnisses von Natur, Kultur und Geschlecht anbieten. Deutlich wurde, dass weiterhin ein kontroverses Ringen um angemessene konzeptionelle Fassungen von Geschlechts- und Naturverhältnissen und die Integration von Gender als zentraler analytischer Querschnitts-Kategorie nötig sind. Insgesamt ist dem Buch ist eine schnelle Verbreitung und

  8. Lightcurve and Rotation Period Determinations for 1599 Giomus and 1888 Zu Chong-Zhi

    Science.gov (United States)

    Foylan, Mike; Rowe, Basil; Smith, Kevin Stephen

    2018-04-01

    Collaborative CCD photometric observations of mainbelt asteroids 1599 Giomus (1950 WA) and 1888 Zu Chong-Zhi (1964 VO1) were acquired during 2017 November and December. A rotation period of 9.53 ± 0.03 h and amplitude of A = 0.06 ± 0.05 mag were determined for 1599 Giomus and 11.053 ± 0.003 h and amplitude of A = 0.56 ± 0.05 mag were determined for 1888 Zu Chong-Zhi.

  9. Zu einigen entwicklungstendenzen beim ausdruck der gewissheits modalität

    Directory of Open Access Journals (Sweden)

    Stojan Bračič

    1981-12-01

    Full Text Available Das verfolgte Ziel in diesem Artikel - einem für den Druck bear­ beiteten Kapitel aus der Magisterarbeit des Verfassers - ist es, möglichst viele Ausdrucksmittel zum Ausdruck der Gewissheits­ modälitat (im weiteren GMO zu finden und sie sys.tematisch zu behandeln. Ausserdem soll durch statistische Bearbeitung der im Korpus gefundenen Belege festgestellt werden, ob man in der Zeit­ spanne derletzten siebzig Jahre beim Ausdruck der GMO in der deutschen Sprache mit Entwicklungstendenzen /ET/ rechnen kann.

  10. Gas chromatography

    International Nuclear Information System (INIS)

    Cram, S.P.; Risby, T.H.; Field, L.R.; Yu, W.L.

    1980-01-01

    In addition to the published literature for the years 1978 - 79, this review of developments in the field of gas chromatography includes earlier articles of particular significance appearing in foreign journals and the patent literature which was not available at the time of the previous review. The articles cited were selected as presenting the most fundamental developments in theory, methodology, and instrumentation. Some applications are cited if they reflect an advance in the state-of-the-art or have particular relevance to new developments. 1149 references are cited

  11. Biotechnologische Konversion von Nebenströmen der Lebensmittelindustrie zu hochwertigen Aromastoffen durch Basidiomyceten

    OpenAIRE

    Kunkel, Katrin

    2013-01-01

    29 Basidiomyceten wurden hinsichtlich der Bildung von natürlichen Aromastoffgemischen in Substrat-supplementierten Submerskulturen untersucht. Als Substrate dienten Karottenschalen, Blattspinat, Sellerietrester, Zwiebelschalen, Lauchtrester und Zitruspellets, die als Reststoffe in der lebensmittelverarbeitenden Industrie anfallen. Die Geruchseindrücke von 61 Biotransformationen wurden im Vergleich zu den Substrat- und Pilz-Blindwerten beurteilt und die Reproduzierbarkeit der ansprechendsten B...

  12. Design of the intake and outlet areas of turbines; Gestaltung der Zu- und Abstroemung von Turbinen

    Energy Technology Data Exchange (ETDEWEB)

    Ruprecht, A. [Stuttgart Univ. (Germany). Abt. Numerische Stroemungsmechanik

    1997-12-31

    Especially in the case of plants with a small height of drop, efficiency can be substantially impaired. Moreover, an unfavourable design of the inlet area can result in operating problems because of instationary flow phenomena. Similar considerations apply to the draft tube and outflow area. Selected examples are chosen to demonstrate problems and propose improvements. As modelling demands too much effort in the case of small plants, the investigations are carried out using modern numerical flow calculation methods. As demonstrated, inlet and outflow areas can be economically designed using these methods even in the case of small hydro power stations so as to present favourable flow parameters. [Deutsch] Vor allem bei Anlagen mit kleiner Fallhoehe kann der Verlust betraechtlich sein. Darueber hinaus kann eine unguenstige Gestaltung des Einlaufbereichs durch instationaere Stroemungsphaenomene auch zu Problemen beim Betrieb der Anlage fuehren. Aehnliches gilt bei der Gestaltung des Saugrohrs und der Abstroemung. An ausgewaehlten Beispielen werden Probleme dargestellt und Verbesserungsvorschlaege aufgezeigt. Da bei kleinen Anlagen Modellversuche zu aufwendig sind, erfolgen die Untersuchungen mit modernen Methoden der numerischen Stroemungsrechnung. Es wird gezeigt, dass man mit diesen Methoden in der Lage ist, auch bei Kleinwasserkraftanlagen wirtschaftlich eine stroemungsguenstige Gestaltung von Zu- und Abstroemung zu erreichen. (orig.)

  13. Gefährlicher Trend : Smartphone im Strassenverkehr : auch zu Fuss ist das Ablenkungspotenzial sehr gross.

    NARCIS (Netherlands)

    2016-01-01

    Viele Menschen, die sich zu Fuß im Straßenverkehr bewegen, nutzen dabei das Smartphone. Dies ist das Ergebnis einer Erhebung von DEKRA in sechs europäischen Hauptstädten. Von den rund 14.000 erfassten Personen nutzten fast 17 Prozent ihr Handy — für Textnachrichten, zum Telefonieren oder Musikhören.

  14. Emotional Branding in der Automobilindustrie: ein Schlüssel zu langfristigem Markenerfolg?

    OpenAIRE

    Nadler, Sabrina; Rennhak, Carsten

    2011-01-01

    In der Automobilindustrie vollzieht sich aktuell ein Wandel von der Technik- zur Kundenorientierung. Die Konsumenten sollen Produkte und Dienstleistungen nicht nur einfach nutzen, sondern eine emotionale Bindung zu ihnen aufbauen und sie im Idealfall sogar lieben. Seit Gobé (2001) werden entsprechende Markenführungsansätze mit dem Schlagwort „Emotional Branding“ bezeichnet.

  15. Neue Welle an IFRS: Wechseln schon bald mehr Firmen zu Swiss GAAP FER?

    OpenAIRE

    Sager, Marc; Vial, Jérôme

    2016-01-01

    Seit 2008 wechseln an der SIX kotierte Firmen vermehrt den Rechnungslegungsstandard von den IFRS zu Swiss GAAP FER (FER). Mit IFRS 9 (Finanzinstrumente), IFRS 15 (Erlöse aus Verträgen mit Kunden) und IFRS 16 (Leasingverhältnisse) werden die Anwender einmal mehr mit weitreichenden Änderungen konfrontiert.

  16. Cromatografia unificada Unified chromatography

    Directory of Open Access Journals (Sweden)

    Carin von Mühlen

    2004-10-01

    Full Text Available The scope of this study encompasses an overview of the principles of unified chromatography as well as the principles of chromatographic techniques as applied to unified systems, which include gas chromatography, liquid chromatography, supercritical fluid chromatography, high temperature and high pressure liquid chromatography, micro-liquid chromatography, enhanced fluidity chromatography, and solvating gas chromatography. Theoretical considerations and individual instrumental parameters such as mobile phase, sample introduction system, columns, and detection system are also discussed. Future applications of this separation approach are discussed.

  17. Rückwärtsintegration - Zu den Verhältnissen Gymnasium, Hochschule und Arbeitswelt

    Science.gov (United States)

    Schmid, Gerhard; Heppner, Winfried; Focht, Eva

    In seiner 2007 erschienen Sammlung von Vorträgen und Essays beschäftigt sich Wolfgang Frühwald, mit der Frage "Wieviel Wissen brauchen wir?“ [1] Die Kernproblematik moderner Wissenschaft und Forschung sieht der Autor, emeritierter Ordinarius für Neuere Deutsche Literaturwissenschaft und von 1992 bis 1997 Präsident der Deutschen Forschungsgemeinschaft, einerseits in der zunehmenden Spezialisierung der Wissenschaftsbereiche, andererseits in der Gefahr der Abkoppelung der Naturwissenschaften von den Geisteswissenschaften. Wiederholt plädiert er dafür, über der rasanten Entwicklung beispielsweise in der Biologie und Physik, die historische, gesellschaftliche und besonders die ethische Dimension der Forschung nicht zu übersehen und fordert eine übergeordnete Theorie der Wissenschaft, die nur im Dialog zwischen den einzelnen Fachgebieten zu entwickeln sei.

  18. Rezension zu: Rita Pawlowski (Hg.: „Unsere Frauen stehen ihren Mann“. Berlin: trafo Wissenschaftsverlag 2008

    Directory of Open Access Journals (Sweden)

    Susanne Freund

    2009-07-01

    Full Text Available Rita Pawlowski stellt in diesem Band in alphabethischer Ordnung insgesamt 651 Frauen vor, die Abgeordnete der Volkskammer der DDR waren. Nach einer knappen Einführung zur Wahlhäufigkeit weiblicher Abgeordneter, zum Frauenanteil in der Volkskammer und in den Ausschüssen geben die Biographen im Wesentlichen Auskunft über die soziale Herkunft, die Parteizugehörigkeit, die schulische und berufliche Ausbildung und die Berufstätigkeit der einzelnen Frauen. Ziel dieses Bandes ist die Dokumentation des außergewöhnlichen Engagements von Frauen in der DDR, neben Berufstätigkeit und Familienpflichten politische Einflussmöglichkeiten zu nutzen. Damit wird ein Teilaspekt der Lebensgeschichte dieser Frauen beleuchtet, der – wie die Autorin im Vorfeld einräumt – keinen Anspruch auf Vollständigkeit erhebt. Dieser Hinweis ist wichtig, um dieses lexikalische Nachschlagewerk sinnvoll zu handhaben.

  19. Web 2.0 Dienste als Ergänzung zu algorithmischen Suchmaschinen

    CERN Document Server

    Deutschland. Bundesministerium für Wirtschaft und Technologie; Maas, Christian

    2008-01-01

    Dokumentiert die Ergebnisse des Fachprojekts "Einbingung von Frage-Antwort-Diensten in die Web-Suche", Wintersemester 2007/2008, Dept. Information, Hochschule für angewandte Wissenschaften Hamburg. Mit sozialen Suchdiensten - wie z.B. Yahoo Clever, Lycos iQ oder Mister Wong - ist eine Ergänzung zu den bisherigen Ansätzen in der Web-Suche entstanden. Während Google und Co. automatisch generierte Trefferlisten bieten, binden soziale Suchdienste die Anwender zur Generierung der Suchergebnisse in den Suchprozess ein. Vor diesem Hintergrund wird in diesem Buch der Frage nachgegangen, inwieweit soziale Suchdienste mit traditionellen Suchmaschinen konkurrieren oder diese qualitativ ergänzen können. Der vorliegende Band beleuchtet die hier aufgeworfene Fragestellung aus verschiedenen Perspektiven, um auf die Bedeutung von sozialen Suchdiensten zu schließen.

  20. Zum Entwicklungsstand und zu Entwicklungsproblemen unserer Jugend - 1987 : Forschungsergebnisse - Beobachtungen - Überlegungen in 10 Thesen

    OpenAIRE

    Friedrich, Walter

    1987-01-01

    Dieser Bericht gibt in zehn Thesen einen Überblick über Forschungsergebnisse, Beobachtungen und Überlegungen zum Entwicklungsstand der DDR-Jugend im Jahre 1987. Diese sei "in ihrer großen Mehrheit durch ein hohes Niveau ihrer sozialistischen Persönlichkeitsentwicklung charakterisiert". Kritisiert wird die Rolle der FDJ, die die Interessenvertretung der Jugend nicht ausreichend wahrnehme. Weiterhin nehme die Rezeption westlicher Rundfunk- und Fernsehsendungen immer mehr zu, jedoch sei es verbo...

  1. Verzwickte Flussgemeinschaft - Warum es so schwierig ist, den Flüssen Raum zu geben

    NARCIS (Netherlands)

    Hartmann, T.

    2011-01-01

    Extreme Hochwasser verursachen enorme Schäden in Flusslandschaften. Flussgemeinschaften müssen künftig den Flüssen mehr Raum geben, um Hochwasser in der Fläche zurückzuhalten, statt in der Höhe (der Deiche) zu bekämpfen. Die Idee „den Flüssen mehr Raum geben“ beschreibt eigentlich etwas

  2. Untersuchungen zu mummy, kette und Interaktionspartnern bei der Entwicklung des Drosophila Nervensystems

    OpenAIRE

    Strunk, J.M. (Judith)

    2006-01-01

    Die Dissertation beinhaltet genetische, funktionelle und phänotypische Analysen von Mutationen, die zu Defekten der Musterbildung des embryonalen ZNS von Drosophila melanogaster führen. Das Gen mummy wurde im Bereich 26D7 lokalisiert und codiert eine UDP-N-ACETYLGLUCOSAMIN-PYROPHOSPHORYLASE. MUMMY katalysiert eine Reaktion, deren Produkt, das UDP-N-Acetylglucosamin, Grundbaustein für Glycosylierung und GPI-Ankersynthese ist. Effekte von mummy auf diese Prozesse konnten dargestellt werden. Für...

  3. Von der Europäischen Rechtsgeschichte zu einer Rechtsgeschichte Europas in globalhistorischer Perspektive

    Directory of Open Access Journals (Sweden)

    Thomas Duve

    2012-01-01

    In den letzten Jahren sind im Zuge der Diskussion um postkoloniale Perspektiven auf die Geschichte, um Transnationale und Globalgeschichte, viele Grundlagen der traditionellen Europahistoriographie kritisiert und massiv erschüttert worden. Das wirft Fragen auch an die Europäische Rechtsgeschichte auf: Welches Europabild liegt ihr zu Grunde? Auf welchen intellektuellen und konzeptionellen Grundlagen beruht sie? Wie antwortet sie auf die Vorwürfe des Eurozentrismus, des epistemischen Kolonialismus, wie auf die Forderung, Europa zu ›provinzialisieren‹? Wie definiert sie das Verhältnis der Europäischen zur Transnationalen und Globalen Rechtsgeschichte? – Diesen und ähnlichen Fragen wenden sich die folgenden Überlegungen zu. Der Schwerpunkt liegt auf einer Auseinandersetzung mit der Tradition, ihren konzeptionellen Grundlagen und deren wissenschaftshistorischem Kontext (1. Teil, 1.–6.. Aus dieser kritischen Bestandsaufnahme und den Ergebnissen der Debatte um Globalgeschichte ergeben sich Ausgangspunkte und Aufgaben einer in Vielem auf den Leistungen der Disziplin aufbauenden, doch notwendigerweise auf einer anderen Konzeption beruhenden Rechtsgeschichte Europas in globalhistorischer Perspektive (2. Teil, 7.–11..

  4. [Von den Restgütern zu den Sowchosen in Estland 1939-1953. Dokumentensammlung] / Olev Liivik

    Index Scriptorium Estoniae

    Liivik, Olev, 1975-

    2011-01-01

    Arvustus: Von den Restgütern zu den Sowchosen in Estland 1939-1953 : Dokumentensammlung / Hrsg. von David Feest, Karsten Brüggemann. Berlin : Lit Verlag, 2010. (Schriften der Baltischen historischen Kommission ; Bd. 15)

  5. Report: Affinity Chromatography.

    Science.gov (United States)

    Walters, Rodney R.

    1985-01-01

    Supports, affinity ligands, immobilization, elution methods, and a number of applications are among the topics considered in this discussion of affinity chromatography. An outline of the basic principles of affinity chromatography is included. (JN)

  6. Zu einer inhaltsorientierten Theorie des Lernens und Lehrens der biologischen Evolution

    Science.gov (United States)

    Wallin, Anita

    Der Zweck dieser Studie (zwecks Überblick siehe dazu Abb. 9.1) war zu untersuchen, wie die Schüler der Sekundarstufe II ein Verständnis von der Theorie der biologischen Evolution entwickeln. Vom Ausgangspunkt "Vorurteile der Schüler“ ausgehend wurden Unterrichtssequenzen entwickelt und drei verschiedene Lernexperimente in einem zyklischen Prozess durchgeführt. Das Wissen der Schüler wurde vor, während und nach den Unterrichtssequenzen mit Hilfe von schriftlichen Tests, Interviews und Diskussionsrunden in kleinen Gruppen abgefragt. Etwa 80 % der Schüler hatten vor dem Unterricht alternative Vorstellungen von Evolution, und in dem Nachfolgetest erreichten circa 75 % ein wissenschaftliches Niveau. Die Argumentation der Schüler in den verschiedenen Tests wurde sorgfältig unter Rücksichtnahme auf Vorurteile, der konzeptionellen Struktur der Theorie der Evolution und den Zielen des Unterrichts analysiert. Daraus konnten Einsichten in solche Anforderungen an Lehren und Lernen gewonnen werden, die Herausforderungen an Schüler und Lehrer darstellen, wenn sie anfangen, evolutionäre Biologie zu lernen oder zu lehren. Ein wichtiges Ergebnis war, dass das Verständnis existierender Variation in einer Population der Schlüssel zum Verständnis von natürlicher Selektion ist. Die Ergebnisse sind in einer inhaltsorientierten Theorie zusammengefasst, welche aus drei verschiedenen Aspekten besteht: 1) den inhaltsspezifischen Aspekten, die einzigartig für jedes wissenschaftliche Feld sind; 2) den Aspekten, die die Natur der Wissenschaft betreffen; und 3) den allgemeinen Aspekten. Diese Theorie kann in neuen Experimenten getestet und weiter entwickelt werden.

  7. Social Entrepreneurship im etablierten Wohlfahrtsstaat : Aktuelle empirische Befunde zu neuen und alten Akteuren auf dem Wohlfahrtsmarkt

    OpenAIRE

    Heinze, Rolf G.; Schönauer, Anna-Lena; Schneiders, Katrin; Grohs, Stephan; Ruddat, Claudia

    2013-01-01

    Im internationalen Vergleich hat sich die wissenschaftliche Diskussion um die gesellschaftliche Relevanz des Phänomens „Social Entrepreneurship“ (SE) in Deutschland relativ spät entwickelt. In Asien wurde die Debatte insbesondere durch die von Muhammad Yunus 1983 gegründete Grameen Bank angestoßen und spätestens seit der Auszeichnung Yunus‘ mit dem Friedensnobelpreis wird SE in vielen Nationen zunehmend als Chance wahrgenommen, soziale Missstände effektiv und nachhaltig zu bekämpfen....

  8. Abfallentsorgung zu Lasten der Bürger? : Probleme der Kreislaufwirtschaft und Lösungsansätze für eine Entlastung der Haushalte

    OpenAIRE

    Kaimer, Martin; Schade, Diethard

    2000-01-01

    Das heutige Abfallentsorgungssystem und die ihm zugrunde liegenden gesetzlichen Regelungen haben eine lange Vorgeschichte und beruhen auf unterschiedlichen Zielsetzungen. Im Ergebnis haben die daraus folgenden Anforderungen zu teilweise fragwürdigen Entsorgungspfaden, zu einem komplizierten Nebeneinander privater und öffentlichrechtlicher Entsorger und vor allem zu höheren Müllgebühren für die Bürger geführt. Ausgehend von den Wirkungen, die mit der Umsetzung der gesetzlichen Regelungen errei...

  9. Vom Geschäftsgang zum Prozess: Prozessmanagement in Bibliotheken am Beispiel der Staatsbibliothek zu Berlin

    Directory of Open Access Journals (Sweden)

    Cornelia Vonhof

    2015-12-01

    Full Text Available Der Beitrag skizziert den Weg der Staatsbibliothek zu Berlin bei der Implementierung eines systematischen Prozessmanagements. Basierend auf den Erfahrungen aus einem Pilotprojekt werden Vorgehensweisen und Methoden reflektiert und weiterentwickelt, die sich eignen, in einer großen, arbeitsteiligen Organisation eingesetzt zu werden. Beleuchtet werden Motivationsfaktoren für Prozessmanagement sowie neue Ansätze wie Service Blueprint und Adaptiv Case Management, die das klassische Verständnis von Prozessmanagement erweitern. The article outlines the path of the Berlin State Library working towards the implementation of a systematic process management. Based on the experience of a pilot project procedures and methods are reflected and further developed to be suitable for a large, work-sharing organization. Motivating factors for process management as well as new approaches such as Service Blueprint and Adaptive Case Management, that expand the classical understanding of process management, are covered.

  10. Philosophisch und theologisch denken (lernen. Fachdidaktische Skizzierungen zu einer ReligionslehrerInnenbildung NEU

    Directory of Open Access Journals (Sweden)

    Philipp Klutz

    2013-10-01

    Full Text Available ENGLISH: It is an essential challenge for (religious teachers to initiate successful learning at school. How teachers act in their instruction is a key factor for successful learning at school. So teacher education has to focus on the process of professionalization of teachers. The faculty of Catholic Theology at the University of Vienna realigns the courses of subject didactic. According to the course ‘Philosophisch und theologisch denken’ this paper illustrates the students’ process of professionalization. The aim of this process is to form a ‘habitus’ in which religious education at schools and subject didactic (didactic of RE is visible under a theological approach. GERMAN: Wie gelingendes Lernen an der Schule initiiert werden kann, ist eine zentrale Herausforderung von (Religions LehrerInnen. Dabei kommt der Qualität des unterrichtlichen Handelns von Lehrpersonen eine entscheidende Rolle zu. In der Ausbildungsphase von ReligionslehrerInnen gilt es, sich dem Prozess der Professionalisierung zu widmen. An der Katholisch-Theologischen Fakultät der Universität Wien wird die fachdidaktische Lehre neu ausgerichtet, die sich so den Herausforderungen des Religionslehrberufs stellt. Anhand des fachdidaktischen Seminars ‚Philosophisch und theologisch denken‘ wird der Professionalisierungsprozess von Studierenden aufgezeigt. Durch diesen soll eine Haltung (Habitus ausgebildet werden, der eine theologisch begründete Sichtweise religiöser Bildung an der Schule und somit von Fachdidaktik (Religionsdidaktik sichtbar werden lässt.

  11. Experimental investigations on the mechanisms of the warm prestress effect; Experimentelle Untersuchungen zu den Mechanismen des WPS-Effekts

    Energy Technology Data Exchange (ETDEWEB)

    Roos, E.; Alsmann, U. [Stuttgart Univ. (Germany). Staatliche Materialpruefungsanstalt

    1998-11-01

    The Government-supported, joint project by IWW Freiburg, BAM Berlin, IWW Magdeburg, and MPA Stuttgart is intended to yield insight into the mechanisms underlying the WPS, warm prestress effect, and to derive on this basis a quantitative description of the WPS effect. (orig./CB) [Deutsch] Ziel eines durch das BMBF gefoerderten Gemeinschaftsprojekts von IWW Freiburg, BAM Berlin, IWW Magdeburg und MPA Stuttgart ist es, die Mechanismen des WPS-(warm prestress)-Effekts zu erklaeren und auf dieser Basis eine quantitative Beschreibung des WPS-Effekts zu ermoeglichen. (orig./MM)

  12. Chromatography resin support

    Science.gov (United States)

    Dobos, James G.

    2002-01-01

    An apparatus and method of using an improved chromatography resin support is disclosed. The chromatography support platform is provided by a stainless steel hollow cylinder adapted for being inserted into a chromatography column. An exterior wall of the stainless steel cylinder defines a groove for carrying therein an "O"-ring. The upper surface of the stainless steel column is covered by a fine stainless steel mesh welded to the edges of the stainless steel cylinder. When placed upon a receiving ledge defined within a chromatography column, the "O"-ring provides a fluid tight seal with the inner edge wall of the chromatography cylinder. The stainless steel mesh supports the chromatography matrix and provides a back flushable support which is economical and simple to construct.

  13. Separation techniques: Chromatography

    Science.gov (United States)

    Coskun, Ozlem

    2016-01-01

    Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Proteins can be purified based on characteristics such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with the stationary phase. Four separation techniques based on molecular characteristics and interaction type use mechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography. Column chromatography is one of the most common methods of protein purification. PMID:28058406

  14. Chromatography of phosphorus oxoacids

    International Nuclear Information System (INIS)

    Ohashi, S.

    1975-01-01

    The present state of studies on the chromatographic separation of phosphorus oxoacids is surveyed. In this paper, chromatographic techniques are divided into four groups, i.e. paper and thin-layer chromatography, paper electrophoresis, ion-exchange chromatography, and gel chromatography. The separation mechanisms and characteristics for these chromatographic methods are discussed and some examples for the separation of phosphorus oxoacids are described. As examples of the application of ion-exchange and gel chromatography, studies on the hot atom chemistry of 32 P in solid inorganic phosphates and those on the substitution reactions between diphosphonate (diphosphite) and polyphosphates are reported. (author)

  15. Ideen zu einer Literatursoziologie des Internets. Mit einer Blogotop-Analyse

    Directory of Open Access Journals (Sweden)

    Peer Trilcke

    2013-11-01

    Full Text Available Der Essay skizziert und exemplifiziert die Möglichkeiten einer Literatursoziologie des Internets und plädiert in diesem Zusammenhang für eine Empirisierung der Forschung zu diesem rasant wachsenden, jedoch bisher in seiner Differenziertheit allenfalls oberflächlich beschriebenen Feld der Kommunikation über Literatur. Hierzu werden die literaturwissenschaftlichen Internet Studies und ihr literatursoziologischer Teilbereich innerhalb der allgemeinen Digital Humanities verortet. Als Beispiel für eine in diesem Sinne relational-empirische Literatursoziologie des Internets werden erste Ergebnisse der Analyse eines sog. Bücherblogotops präsentiert, anhand derer sich einige Mechanismen der sekundären literarischen Kommunikation zwar nicht repräsentativ, aber doch exemplarisch diskutieren lassen.

  16. Cyborg oder Göttin: Wie Technikforscherinnen ihr Verhältnis zu Technik sehen

    Directory of Open Access Journals (Sweden)

    Ulrike Kissmann

    2001-11-01

    Full Text Available Die Autorinnen haben für die Beiträge eigene auto/biographische Erlebnisse ausgewählt, in denen der mediale Charakter von Technik deutlich wird. Die Geschichten erzählen also von Technik, die soziale Ordnung und Bedeutung vermittelt und auf diese Weise dazu beiträgt, Geschlechtergrenzen und andere Grenzen wie die zwischen „bekannt“ und „fremd“ oder „normal“ und „anormal“ herzustellen. Die Auto/Biographien sind durch Technik einerseits fremdbestimmt, andererseits zeigen die Autorinnen auch, wie Selbstbestimmung über diese Grenzen hinweg möglich ist. Sie benutzen Donna Haraways Cyborg Metapher (1985, um das Selbst in der Verflechtung von Sozialem und Technischem auto/biographisch zu rekonstruieren.

  17. Analyse der Literatur zu Klimawirkungen in Deutschland: ein Gesamtbild mit Lücken

    Science.gov (United States)

    Fleischhauer, Mark; Greiving, Stefan; Lindner, Christian; Lückenkötter, Johannes; Schauser, Inke

    Dieses Kapitel präsentiert Ergebnisse einer umfassenden Literaturauswertung zu relevanten Klimawirkungen für Deutschland, die im Rahmen des Projekts "Netzwerk Vulnerabilität" vorgenommen wurde. Es zeigt auf, dass ein aggregiertes Gesamtbild der Klimawirkungen für Deutschland als Grundlage für Folgenabschätzungen und Anpassungsplanungen noch nicht gezeichnet werden kann, da eine große Bandbreite an Ansätzen zur Bewertung von Vulnerabilitäten oder Klimawandelfolgen existiert und die gegenwärtig vorhandenen Klimawirkungs- und Vulnerabilitätsstudien von großer Heterogenität gekennzeichnet sind. Als erster Schritt wird deshalb eine Zusammenschau bereits vorhandener Ansätze geliefert.

  18. Quantenphysik und Kommunikationswissenschaft auf dem Weg zu einer allgemeinen Theorie der Kommunikation

    CERN Document Server

    Hamberger, Erich

    2015-01-01

    Der Quantenphysiker Herbert Pietschmann beschäftigt sich seit Jahrzehnten mit dem Phänomen Kommunikation. Der Kommunikationswissenschaftler Erich Hamberger setzt sich seit langem mit der Frage der Adaptierung erkenntnistheoretischer Einsichten der Quantentheorie für die Geistes- und Biowissenschaften auseinander. 2003 begegnen sich die beiden - und staunen über die „verschränkten Erkenntnisinteressen“. 2006 bestreiten sie eine erste gemeinsame universitäre Lehrveranstaltung. 2011 folgt Das Phänomen Kommunikation transdisziplinär betrachtet. Aus ihrer Zusammenarbeit ist dieses Buch entstanden. Naturwissenschaft arbeitet auf der Grundlage des mechanistischen Denkrahmens. Die bisher einzige Ausnahme ist die Quantenphysik. Weder Leben noch Kommunikation ist mittels des mechanistischen Denkrahmens zu verstehen. Auch der Denkrahmen der Quantenphysik reicht dazu nicht aus. Der Bedarf nach adäquatem Denken im Bereich der Kommunikation kann durch quantenphysikalisches Denken NICHT befriedigt werden, jedoc...

  19. Auf dem Weg zu semantischen APIs für Forschungsdatendienste

    Directory of Open Access Journals (Sweden)

    Anna Fensel

    2017-09-01

    Full Text Available Die schnelle Entwicklung der Internet- und Web-Technologie verändert den Stand der Technik in der Kommunikation von Wissen oder  Forschungsergebnissen. Insbesondere werden semantische Technologien, verknüpfte und offene Daten zu entscheidenden Faktoren für einen  erfolgreichen und effizienten Forschungsfortschritt. Zuerst definiere ich den Research Data Service (RDS und diskutiere typische aktuelle  und mögliche zukünftige Nutzungsszenarien mit RDS. Darüber hinaus bespreche ich den Stand der Technik in den Bereichen semantische Dienstleistung und Datenanmerkung und API-Konstruktion sowie infrastrukturelle Lösungen, die für die RDS-Realisierung anwendbar sind. Zum Schluss werden noch innovative Methoden der Online-Verbreitung, Förderung und effizienten Kommunikation der Forschung diskutiert.

  20. Euphemismen und Dysphemismen im Flexibilisierungsdiskurs. Auf dem Weg zu einem mehrperspektivischen Untersuchungsdesign

    Directory of Open Access Journals (Sweden)

    Goranka Rocco

    2015-07-01

    Full Text Available Abstract – This article outlines  theoretical and empirical prolegomena to the discourse-analytical study of euphemisms and dysphemisms which appear in the discourse of ‘flexibility’. After giving an operative definition of the euphemisms and dysphemisms and discussing some epistemological and other problems, the article presents an „EuDy-Modell“ called framework for studying euphemisms and dysphemisms from the perspective of discourse linguistics.Abstract – Der Beitrag enthält theoretische und empirische Prolegomena zu einer diskurslinguistisch orientierten Untersuchung von Euphemismen und Dysphemismen im Flexibilisierungsdiskurs. Zunächst werden eine funktionale Arbeitsdefinition der Begriffe Euphemismus und Dysphemismus gegeben und einige epistemologische und andere Probleme des Forschungsvorhabens erörtert, um im Anschluss ein Modell zur Analyse von Euphemismen und Dysphemismen aus der diskurslinguistischen Perspektive – das „EuDy-Modell“ – vorzustellen.

  1. ALBA, organisation interaméricaine ou vénézuélienne ?

    Directory of Open Access Journals (Sweden)

    Jean-Jacques Kourliandsky

    2011-01-01

    Full Text Available L’«Alliance Bolivarienne des peuples de notre Amérique», ou ALBA, est de toutes les organisations interaméricaines celle dont l’identité est la plus originale. S’affirmant anti-impérialiste elle donne en effet à l’idéologie une place centrale qui commande la coopération entre ses membres. Mais l’ALBA au quotidien diffère-t-elle d’autres organisations d’intégration ? Le lien entre les différents pays qui la composent n’est-il pas au-delà de l’hommage unanime rendu à Bolivar bien davantage celui de pouvoir bénéficier du pétrole vénézuélien à un prix préférentiel ? L’ALBA, comme d’autres institutions latino-américaines, ne répond-elle pas à l’ambition d’un État, en l’occurrence ici le Venezuela, plus qu’à celle de construire un projet collectif pérenne? Ce lien entre le pétrole de son initiateur vénézuélien et l’ALBA, est une garantie pour le présent. Mais l’ALBA survivrait-elle à une alternance politique au Venezuela, ou à une chute des prix du baril affectant le nerf de l’organisation ?

  2. The new IEA research programme on PV systems in buildings; Das neue Forschungsprogramm der internationalen Energieagentur zu Photovoltaik an Gebaeuden

    Energy Technology Data Exchange (ETDEWEB)

    Laukamp, H.; Erge, T. [Fraunhofer-Inst. fuer Solare Energiesysteme, Freiburg (Germany). Abt. fuer Photovoltaische Systeme und Messtechnik

    1998-02-01

    The International Energy Agency coordinates and supports the cooperation of OECD countries in energy politics and energy technologies by technology-oriented scientific programmes (`implementing agreements`). Under these agreements subject-oriented scientific projects (`tasks`) are carried out. Within the `Photovoltaic Power Systems Programme` the Task VII (Photovoltaics in the Built Environment) has just begun. The Fraunhofer ISE was contracted to coordinate the German contribution to Task VII and to organize information transfer to interested German institutions. So far Task VII focussed on a selection of architecturally outstanding PV buildings, on developing criteria to assess their quality and on a critical review of planned PV buildings. (orig.) [Deutsch] Die Internationale Energieagentur foerdert die Zusammenarbeit der OECD Laender in der Energiepolitik und bei den Energietechnologien, durch gemeinsam vereinbarte technologiespezifische Programme (`Implementing Agreements`). Die Programme werden durch Projekte (`Tasks`) konkretisiert. Im Programm `Photovoltaic Power Systems` wird derzeit Task VII `Photovoltaics in the Built Environment` begonnen. Das Fraunhofer ISE wurde gebeten, die deutsche Beteiligung hieran zu koordinieren und den Informationstransfer zu interessierten deutschen Firmen und Instituten zu organisieren. Schwerpunkte bisheriger Arbeiten lagen bei der Auswahl architektonisch herausragender PV-Gebaeude, bei der Erarbeitung von Kriterien zu ihrer Beurteilung und bei der kritischen Diskussion geplanter PV-Gebaeude. (orig.)

  3. Liquid Chromatography in 1982.

    Science.gov (United States)

    Freeman, David H.

    1982-01-01

    Reviews trends in liquid chromatography including apparatus, factors affecting efficient separation of a mixture (peak sharpness and speed), simplified problem-solving, adsorption, bonded phase chromatography, ion selectivity, and size exclusion. The current trend is to control chemical selectivity by the liquid phase. (Author/JN)

  4. Column Liquid Chromatography.

    Science.gov (United States)

    Majors, Ronald E.; And Others

    1984-01-01

    Reviews literature covering developments of column liquid chromatography during 1982-83. Areas considered include: books and reviews; general theory; columns; instrumentation; detectors; automation and data handling; multidimensional chromatographic and column switching techniques; liquid-solid chromatography; normal bonded-phase, reversed-phase,…

  5. „Wir verbrachten mehr als 24 Stunden, ohne etwas anderes als Schokolade und Limonade zu uns zu nehmen“. Hinweise in Alexander von Humboldts Tagebuchaufzeichnungen zu Fragen der Verpflegung auf der Forschungsreise durch Spanisch-Amerika

    Directory of Open Access Journals (Sweden)

    Ursula Thiemer-Sachse

    2013-12-01

    Full Text Available Zusammenfassung In Alexander von Humboldts Tagebüchern seiner berühmten Forschungsreise durch Spanisch-Amerika finden sich Bemerkungen und Hinweise für künftige Leser seiner Reisedarstellungen zu Problemen der Verpflegung. Zumeist werden Engpässe und Missstände erwähnt sowie Fragen soziopolitischer Situation verdeutlicht. Sie beziehen sich auf Extrembedingungen in stadtfernen Gebieten und im Kontakt mit den indigenen Trägern und Helfern während der einzelnen Exkursionen. Die Darstellungen der alltäglichen Gegebenheiten sind zugleich eine interessante Quelle für Verhaltensmuster der verschiedenen Schichten der spanischkolonialen Gesellschaft wie der Forschungsreisenden, die mit den Schwierigkeiten umzugehen hatten, sich anzupassen verstanden oder ihre eigenen Verhaltensmuster entwickelt haben. Damit ermöglichen Humboldts Aufzeichnungen zugleich auch auf diesem Gebiet manchen Blick auf den seither erfolgten Kulturwandel. Resumen En los diarios del famoso viaje de estudios de Alejandro de Humboldt por la América española se encuentran observaciones y notas sobre problemas de aprovisionamiento, en favor de lectores futuros de sus relaciones de viaje. En la mayoría de los casos Humboldt menciona situaciones precarias o ilustra cuestiones de carácter sociopolítico. Frecuentemente, sus apuntes se refieren a condiciones extremas en regiones remotas y en contacto con los cargadores y ayudantes indígenas durante las diferentes excursiones. Las descripciones de los acontecimientos cotidianos al mismo tiempo son una fuente interesante sobre los modos de proceder de las diferentes capas de la sociedad colonial española así como de los viajeros científicos que tuvieron que enfrentar dificultades a las cuales había que adaptarse o desarrollar modelos propios de comportamiento. Por esto al mismo tiempo las noticias de Humboldt también en este asunto posibilitan más de una vez la atención al cambio cultural que se ha realizado hasta hoy

  6. Gesundheits- und Konzentrationsfähigkeitsuntersuchungen bei Büroangestellten im Rahmen einer Pilotstudie zu Exposition gegenüber tonerbasierten Bürogeräten

    OpenAIRE

    Schumann, Henrik

    2011-01-01

    Ziel der vorliegenden Arbeit war es zu untersuchen, ob und gegebenenfalls in welchem Maße der Betrieb von Laserdruckern und Fotokopiergeräten in Büro-räumen Einfluss auf die von Büroangestellten geäußerten Konzentrations- und Gesundheitsbeschwerden hat. Die Studie wurde dabei als eine Pilotstudie an-gelegt, die primär zur Aufgabe hatte, Erhebungsinstrumente auszuwählen, wei-terzuempfehlen und auf ihre Einsetzbarkeit zu überprüfen. Zu diesem Zwecke wurden zwischen Januar und Oktober 2006 in Ko...

  7. Gas chromatography in space

    Science.gov (United States)

    Akapo, S. O.; Dimandja, J. M.; Kojiro, D. R.; Valentin, J. R.; Carle, G. C.

    1999-01-01

    Gas chromatography has proven to be a very useful analytical technique for in situ analysis of extraterrestrial environments as demonstrated by its successful operation on spacecraft missions to Mars and Venus. The technique is also one of the six scientific instruments aboard the Huygens probe to explore Titan's atmosphere and surface. A review of gas chromatography in previous space missions and some recent developments in the current environment of fiscal constraints and payload size limitations are presented.

  8. Supercritical fluid chromatography

    Science.gov (United States)

    Vigdergauz, M. S.; Lobachev, A. L.; Lobacheva, I. V.; Platonov, I. A.

    1992-03-01

    The characteristic features of supercritical fluid chromatography (SCFC) are examined and there is a brief historical note concerning the development of the method. Information concerning the use of supercritical fluid chromatography in the analysis of objects of different nature is presented in the form of a table. The roles of the mobile and stationary phases in the separation process and the characteristic features of the apparatus and of the use of the method in physicochemical research are discussed. The bibliography includes 364 references.

  9. Der kleine Unterschied. Zu den Selbstverhältnissen von Verantwortung und Pflicht

    Directory of Open Access Journals (Sweden)

    Vogelmann Frieder

    2015-12-01

    Full Text Available Die Debatte um die Differenz von „Verantwortung“ und „Pflicht“ ist kein bloßer Streit um Wörter, geht es doch um Begriffe, für die der Anspruch erhoben wird, sie seien konstitutiv für moralische Normativität oder gar für Normativität per se. Doch welchen Unterschied macht es, die besondere Bindungskraft von Normativität über Verantwortung oder über Pflicht zu explizieren? Die Genealogie der philosophischen Reflexionen auf Verantwortung lokalisiert die Differenz zwischen Pflicht und Verantwortung in den jeweiligen Selbstverhältnissen, die mit diesen Begriffen verbunden werden. Die Analyse der Struktur dieser Selbstverhältnisse erklärt sowohl, warum Verantwortung häufig als moderner Ersatzbegriff für Pflicht gedacht wird, als auch, welchen Unterschied die Explikation der Bindungskraft von Normativität über Verantwortung oder Pflicht macht: Je nachdem, mit welchem Begriff normative Kraft erläutert wird, wandert das entsprechende Selbstverhältnis in die Verfasstheit von Normativität ein.

  10. Medienbildung als konstruktive Aneignung von Medienensembles. Vorbemerkungen zu einer Theorie der Medienpädagogik

    Directory of Open Access Journals (Sweden)

    Tilman Bechthold-Hengelhaupt

    2016-12-01

    Full Text Available Die These dieses Aufsatzes lautet, dass im medienpädagogischen Handeln und Kommunizieren Erfahrungen mit dinglich gegebenen Medien verarbeitet und dass dadurch Medienensembles konstruiert werden, also praktisch verfügbare Konfigurationen von Geräten und Gegenständen, unter denen die beteiligten Akteure eine Auswahl treffen können. Der Begriff der Wahl lässt insbesondere einen Anschluss an die Idee einer demokratischen Medienbildung zu. Als erkenntnistheoretische Grundlage wird eine Orientierung an der sinnlichen Erfahrung vorgeschlagen, die gerade in Immanuel Kants "Kritik der reinen Vernunft" als Erkenntnisquelle begründet wird. Dass der Begriff der Medienensembles auch die historische Dimension aufschließen kann, wird am Beispiel des so genannten Neumagener Schulreliefs gezeigt, einem archäologischen Dokument aus der Zeit um 180 n.Chr. This essay proposes the thesis that media education should be understood as consisting in negotiations about experiences with media ensembles. In a constructivist approach, the acting with media ensembles is seen as a form of coming to terms with devices and other media objects that are accessible via sensory experience. In this context, a pivotal idea is that choices are made between different media that are at hand for educational purposes. This concept is further elaborated in the concepts of Immanuel Kant's theory of perception. As an example, an archaeological monument from ca. 180 AD ("Neumagener Schulrelief" is described and interpreted.

  11. Rezension zu: Christina Holtz-Bacha (Hg.: Frauen, Politik und Medien. Wiesbaden: VS Verlag für Sozialwissenschaften 2008

    Directory of Open Access Journals (Sweden)

    Tanja Maier

    2009-03-01

    Full Text Available In dem von Christina Holtz-Bacha herausgegebenen Sammelband wird aus einer kommunikationswissenschaftlichen Perspektive untersucht, wie Politikerinnen in höchsten politischen Ämtern medial repräsentiert werden. In den einzelnen Beiträgen wird der Frage nachgegangen, ob die zunehmende Sichtbarkeit von Politikerinnen wie Angela Merkel, Christina Kirchner oder Ségolène Royal auch zu einer veränderten medialen Berichterstattung geführt habe. Analysiert werden nicht nur die mediale Präsenz von Politikerinnen im Vergleich zu ihren männlichen Kollegen, sondern auch die Gemeinsamkeiten und Unterschiede in den textuellen und bildlichen Darstellungsweisen. Der Band liefert aus der Perspektive der politischen Kommunikationsforschung einige interessante empirische Einzelanalysen. Die theoretische Auseinandersetzung mit Konzepten der Geschlechterforschung spielt nur am Rande eine Rolle.

  12. Rezension zu: Yvonne Haffner, Beate Krais: Arbeit als Lebensform? Frankfurt am Main u.a.: Campus Verlag 2008

    Directory of Open Access Journals (Sweden)

    Daniela Heitzmann

    2009-07-01

    Full Text Available Nachdem der Mythos des unerbittlich arbeitenden Wissenschaftlers, der sich mit Haut und Haar ‚seiner‘ Forschung verschrieben hat, langsam zu bröckeln beginnt, stellt sich aus der Genderperspektive die Frage nach der Bedeutung der Arbeitskultur für die fortbestehende Unterrepräsentanz von Frauen in der Wissenschaft, besonders in leitenden Positionen. Gleichzeitig trägt die allmähliche ‚Entdeckung‘ des Privaten für Männer, die nun auch Väter und Lebenspartner sein sollen (können, zur Entmythologisierung bei. Der vorliegende Sammelband widmet sich dem Balance-Akt zwischen Arbeits- und Privatleben, den Frauen und Männer zu leisten haben. Auf Grundlage zahlreicher empirischer Untersuchungen wird ein umfassendes und erkenntnisreiches Panorama über die Verquickungen individueller und struktureller Anforderungen an berufstätige Akademikerinnen und Akademiker vorgelegt.

  13. Rezension zu: Uta Fenske: Mannsbilder. Eine geschlechterhistorische Betrachtung von Hollywoodfilmen 1946–1960. Bielefeld: transcript Verlag 2008.

    Directory of Open Access Journals (Sweden)

    Asokan Nirmalarajah

    2009-10-01

    Full Text Available Uta Fenske beschäftigt sich in ihrer Dissertation mit den Männlichkeitsentwürfen des klassischen Hollywoodfilms der 1950er Jahre. Die Historikerin blickt damit zurück in eine Ära US-amerikanischer Geschichte, in der soziokulturelle Umbrüche zu einer Erschütterung tradierter Männlichkeitsbilder, zu einem Krisendiskurs über den modernen Mann führten. Fenskes kulturwissenschaftliche Studie kann sich dabei aus zweierlei Sicht profilieren. Zum einen erweitert sie mit der unorthodoxen Wahl ihres Untersuchungsgegenstandes das geschichtswissenschaftliche Quellenkorpus. Zum anderen kann sie durch ihren Rekurs auf eine Vielzahl von Genres zeigen, dass die Hollywoodfilme der 1950er eine größere Fülle an Antworten auf die Krise anbieten als gemeinhin angenommen.

  14. Beratung und Coaching zum Fremdsprachenlernen : Konzepte, Qualitätssicherung, praktische Erfahrungen : Beiträge zu einer Arbeitstagung (Hannover 2015)

    OpenAIRE

    Böcker, Jessica; Saunders, Constanze; Koch, Lennart; Langner, Michael (Hrsg.)

    2017-01-01

    Dieser Band entstand im Anschluss an eine Tagung zu Sprachlernberatung, die im Juli 2015 an der Leibniz-Universität Hannover stattgefunden hat. Die wesentlichen Beiträge aus drei Sektionen behandeln theoretisch-konzeptionelle Aspekte neben Fragen der Qualitätssicherung und Beispielen von good practice. Der Band gliedert sich dementsprechend in drei Bereiche: Konzepte und Forschung behandelt Fragen der Definitionen und der Konzepte zu Sprachlernberatung bzw. -coaching und ihre wissenschaft...

  15. Die Digitale Bibliothek der Akademie der Wissenschaften zu Göttingen

    Directory of Open Access Journals (Sweden)

    Daniel Beucke

    2014-12-01

    Full Text Available Die Akademie der Wissenschaften zu Göttingen (AdWG und die niedersächsische Staats- und Universitätsbibliothek Göttingen (SUB haben ihre bestehende Kooperation zum Webportal der AdWG intensiviert, das einen Überblick über die Aktivitäten der Göttinger Akademie und umfangreiche Information zu den zahlreichen Langzeitvorhaben bietet und die digitalen Publikationen präsentiert. Zentraler Bestandteil des Webportals ist die Digitale Bibliothek der AdWG, für die im Rahmen der Kooperation neue Präsentationsformen für die Forschungsdaten aus den Akademie-Vorhaben konzipiert und entwickelt wurden. Ein Beispiel für die Umsetzung neuer Präsentationsformen im Portal ist das Edfu-Projekt, dessen Ziel eine Gesamtübersetzung aller Inschriften des Tempels von Edfu in Oberägypten ist. Hinzugezogen werden dazu alle internen, greifbaren, in den Schriften enthaltenen Parallelen, auf die online über das Webportal zugegriffen werden kann. Zerstörte Bereiche lassen sich dadurch oftmals ebenso ergänzen, wie zunächst unverständliche Textpassagen mit Sinn versehen werden können. Nicht nur die bereits vorübersetzten Texte sind auf diese Weise abrufbar, sondern darüber hinaus zahlreiche zusätzliche Materialien, wie z.B. das Fotoarchiv mit seinen mehr als 20.000 Bildern. Die Vernetzung all dieser Daten schafft einen für die Wissenschaft ungewöhnlich tiefen Einblick in die Planung und Konzeption eines altägyptischen Tempels, seiner religiösen Hintergründe und historischen Zusammenhänge. The Göttingen Academy of Sciences and Humanities (AdWG and the Göttingen State and University Library (SUB have intensified their existing cooperation on the web portal of the AdWG. The portal provides an overview of the activities of the Göttingen Academy as well as extensive information on its numerous long-term projects. It also presents the academy’s digital publications. A crucial part of the web portal is the Digital Library of the AdWG. Within the

  16. Wie viel muss ich wissen, um global handeln zu können? Verantwortung für Weltarmut und das Problem der epistemischen Überforderung

    Directory of Open Access Journals (Sweden)

    Weber-Guskar Eva

    2015-12-01

    Full Text Available Was heißt es, sich in unserer globalisierten Welt als eine vollverantwortliche Person zu verstehen und zu verhalten? Einerseits scheint es richtig, dass wir global verantwortlich sind, d.h. dass wir auch gegen entferntes Leid etwas tun sollten; andererseits aber ist wegen vielfacher Überforderungsproblemen unklar ist, wie man diese Verantwortung tatsächlich übernehmen können soll – was wiederum dagegen spricht, dass wir diese Verantwortung berechtigtermaßen zuschreiben können. Um einen Aspekt dieses großen Themas zu behandeln, konzentriere ich mich in diesem Aufsatz auf den Anwendungsbereich von extremer Armut und argumentiere in folgenden Schritten. Zuerst spitze ich das Thema zu auf ein Problem, das Samuel Scheffler in diesem Zusammenhang konstatiert hat, nämlich einen Widerstreit zwischen dem, was Verantwortung unter den Bedingungen der Globalisierung bedeutet und dem, wie wir uns grundsätzlich als Handelnde zu verstehen gewohnt sind. Dann zeige ich erstens, dass dem Thema einer epistemischen Überforderung dabei entscheidendes Gewicht zugesprochen wird, zweitens jedoch, dass sich, genauer besehen, dieses Problem gar nicht in dieser Art stellt. Dafür schlage ich eine Analyse des Verantwortungsbegriffs vor, die es ermöglicht, Kriterien zu identifizieren, anhand derer gewisse Grenzen gezogen werden können, so dass die Zuschreibung der Verantwortung gerechtfertigt werden kann.

  17. Was der Religionspädagogik zu denken gibt. Exegetische Denkanstöße.

    Directory of Open Access Journals (Sweden)

    Jeremia Josef M. Mayr

    2014-07-01

    Full Text Available ENGLISH: The following article addresses three exegetical impulses which should be understood as food for thought to religious education. The first concentrates on the relation between exegesis and religious education because of the importance of reflecting on the concrete design of this special relation. The second centers what is indeed the centre of exegesis and religious education: the biblical text itself and the question of its real role and ways to handle it. Finally follows the presentation of a model of interaction with texts trying to equally respect all participants of this process. Simultaneously, this approach arranges a scope for the concrete biblical text allowing it to speak. This model is composed of three central steps: analysis (an exactly methodic procedure, interpretation (based on analysis, posing the question of meaning and appropriation (individually existential meaning. DEUTSCH: Der vorliegende Beitrag möchte aus exegetischer Perspektive drei Denkanstöße an die Religionspädagogik richten. Zunächst wird der Blick auf das Verhältnis zwischen Exegese und Religionspädagogik gelenkt, denn die Gestaltung dieses Verhältnisses ist entscheidend. Der zweite Denkanstoß rückt in die Mitte, was tatsächlich Mittelpunkt der Exegese und der Bibeldidaktik ist: den Text der Bibel selbst und die Frage, wie mit ihm umgegangen wird. Danach soll ein Modell der Textannäherung vorgestellt werden, das es ermöglichen will, mit Blick auf die Beteiligten auch den Texten genügend Raum zu geben, damit sie tatsächlich sprechen können. Die drei zentralen Teile dieses Modells sind Analyse (genaues methodisches Vorgehen, Interpretation (darauf aufbauend die Frage nach der Bedeutung und Aneignung (persönlich-existentielle Bedeutung.

  18. Extraction chromatography of actinides

    International Nuclear Information System (INIS)

    Muller, W.

    1978-01-01

    Extraction chromatography of actinides in the oxidation state from 2 to 6 is reviewed. Data on using neutral (tbp), basic (substituted ammonium salts) and acidic [di-(2-ethylhexyl)-phosphoric acid (D2EHPA)] extracting agents ketones, esters, alcohols and β-diketones in this method are given. Using the example of actinide separation using D2EHPA, discussed are factors influencing the efficiency of their chromatography separation (nature and particle size of the carrier materials, extracting agents amount on the carrier, temperature and elution rate)

  19. Micellar liquid chromatography

    International Nuclear Information System (INIS)

    Basova, Elena M; Ivanov, Vadim M; Shpigun, Oleg A

    1999-01-01

    Background and possibilities of practical applications of micellar liquid chromatography (MLC) are considered. Various retention models in MLC, the effects of the nature and concentration of surfactants and organic modifiers, pH, temperature and ionic strength on the MLC efficiency and selectivity are discussed. The advantages and limitations of MLC are demonstrated. The performance of MLC is critically evaluated in relationship to the reversed-phase HPLC and ion-pair chromatography. The potential of application of MLC for the analysis of pharmaceuticals including that in biological fluids and separation of inorganic anions, transition metal cations, metal chelates and heteropoly compounds is described. The bibliography includes 146 references.

  20. performance liquid chromatography

    African Journals Online (AJOL)

    user

    2010-11-22

    Nov 22, 2010 ... ISSN 1684–5315 © 2010 Academic Journals. Full Length Research Paper. Determination ... Key words: Processed food, high-performance liquid chromatography, acrylamide, health hazard. INTRODUCTION. In the year 2002, the ... potatoes, breakfast cereals etc. It was thus confirmed that acrylamide has ...

  1. Stille wird zu Farben : [aus "Vaikus saab värvideks" ("Stille wird zu Farben")] : [luuletused] / Jaan Kaplinski ; tlk. Irja Grönholm ja Gisbert Jänicke

    Index Scriptorium Estoniae

    Kaplinski, Jaan, 1941-

    2006-01-01

    Sisu: Stille wird zu Farben ; Der letzte rote Apfel ; Wer allein bleibt ; Ruf nach dem Vorherigen ; Mit jedem Frühling ; So viele Namen ; In die Spuren ; Höher ; Es wird Licht ; Mit dem Sommerwrind um die Wette ; Ob sie noch hört. Orig.: Vaikus saab värvideks ; Viimane verev õun ; Üksi jääb ; Hüüab eelmist ; Iga kevadega ; Niipalju nimesid ; Astub tuule jälgedele ; Käib kõrgemalt ; Saab valguseks ; Suvetuulega võidu ; Kas kuuleb enam

  2. Brief Study and Spanish Translation of Die Kunst das Clavier zu Spielen by Friedrich Wilhelm Marpurg (Berlin, Henning, 1750

    Directory of Open Access Journals (Sweden)

    María de las Nieves Pascual León

    2017-01-01

    Full Text Available This paper includes the fi rst Spanish version of F. W. Marpurg’s treaty Die Kunst das Clavier zu spielen, which had been restricted for the common Spanish-speaking reader since its fi rst appearance in 1750. This philological translation of the treaty (with the facsimile of the fi rst edition and its Spanish translation on the opposite page is instroduced by a brief study of the text’s various editions published during the author’s life, and pretends to offer an initial approach to the treaty, to facilitate the public’s access to the work and to contribute to its full disclosure.

  3. Jenseits von "Identität"? : Zu den Un/Möglichkeiten nicht-identitärer Strategien politischen Handelns

    OpenAIRE

    Rostock, Petra

    2014-01-01

    Kulturalisierungen und Zuschreibungen ›kollektiver Identitäten‹ dienen in Debatten um die Einwande­rungsgesellschaft Deutschland immer wieder dazu eine soziale Ordnung zu konstruieren, die zwischen denen unterscheidet, die dazu gehören und jenen, die nicht dazu gehören. Gleichzeitig formiert sich ›Identitätspolitik‹ als eine widerständige politische Praxis. Sie greift im Bewusstsein einer gemeinsamen Geschichte der Ausbeutung und Unterdrückung infolge einer zugewiesenen und konstruierten ›Ide...

  4. Chromatography in Industry

    Science.gov (United States)

    Schoenmakers, Peter

    2009-07-01

    This review focuses on the chromatography research that has been carried out within industry or in close cooperation with industry and that has been reported in the scientific literature between 2006 and mid-2008. Companies in the health care sector, such as pharmaceutical and biotechnology companies, are the largest contributors. Industrial research seems to take place in an open environment in cooperation with academia, peer companies, and institutions. Industry appears ready to embrace new technologies as they emerge, but they focus strongly on making chromatography work robustly, reliably, rapidly, and automatically. “Hyphenated” systems that incorporate on-line sample-preparation techniques and mass-spectrometric detection are the rule rather than the exception. Various multidimensional separation methods are finding numerous applications. Strategies aimed at speeding up the development of new chromatographic methods remain the focus of attention. Also, there is a clear trend toward exploring chromatographic methods for parallel processing along with other strategies for high-throughput analysis.

  5. Wie "zukunftsreich" ist das neue Lehramtsstudium? Bestandsaufnahme zu Medienbildung und digitalen Kompetenzen in den Curriculaentwürfen der Sekundarstufe der PädagogInnenbildung_NEU

    Directory of Open Access Journals (Sweden)

    Klaus Himpsl-Gutermann

    2015-12-01

    Full Text Available Durch die PädagogInnenbildung_NEU eröffnet sich derzeit die Chance, österreichweit in vier Verbundregionen in Kooperation zwischen Universitäten und Pädagogischen Hochschulen die Lehramtsausbildung für alle Schulstufen und Schultypen zukunftsweisend zu reformieren. Zu den Ansprüchen an das neue Studium gehört auch, dass es künftige LehrerInnen an die Schulen entsendet, die neben ihrer fachlichen Expertise mit aktuellen gesellschaftlichen Herausforderungen und Entwicklungen adäquat umgehen können. Die rasant voranschreitende Technologisierung und Digitalisierung unserer Gesellschaft gehört zweifelsohne zu diesen Herausforderungen. Die E-Learning-Strategiegruppe der österreichischen Pädagogischen Hochschulen (PHELS hat deshalb die aktuell vorliegenden Curricula und Curriculaentwürfe der Sekundarstufe Allgemeinbildung in den vier Verbundregionen einer kritischen Analyse hinsichtlich Medienbildung und digitaler Kompetenzen unterzogen − mit einem ernüchternden bis verheerenden Ergebnis: Es scheint nicht nur die historische Chance vertan, Medienbildung systematisch in der LehrerInnenausbildung und damit in den Schulen zu verankern, sondern durch die fehlende informatische Grundbildung laufen künftige Generationen auch Gefahr, auf zunehmend digitalisierten Arbeitsmärkten den internationalen Anschluss zu verlieren.

  6. Die Erforschung kollektiver und individueller Dynamik – Zu einer historisch und prozess-soziologisch orientierten interpretativen Sozialforschung

    Directory of Open Access Journals (Sweden)

    Gabriele Rosenthal

    2016-04-01

    Full Text Available In diesem Beitrag konzentriere ich mich auf die Vorteile einer konsequent durchgeführten interpretativen oder rekonstruktiven Sozialforschung gegenüber anderen qualitativen Verfahren und verdeutliche, inwiefern wir diese Vorteile noch konsequenter verfolgen können als bisher. Dabei stellen sich die folgenden Fragen: Was können wir mit wenigen, aber aufwendigen Fallrekonstruktionen mit dem Ziel einer "theoretischen Verallgemeinerung" am Einzelfall im Unterschied zu qualitativen Verfahren mit umfangreichen Stichproben leisten, die eher eine numerische oder quantitative Verallgemeinerung zum Ziel haben? Inwiefern kommen wir damit überhaupt zu "allgemeinen" Aussagen über die soziale (kollektive und individuelle Wirklichkeit, über eine bestimmte Gruppierung, Organisation oder Ortsgemeinde, einen kollektiver Konflikt, ein bestimmtes Milieu oder soziales Netzwerk? Bei der Behandlung dieser Fragen geht es mir vor allem um ein Plädoyer für eine methodisch stärker historisch und prozess-soziologisch orientierte interpretative Sozialforschung. M.E. liegen in einer konsequenten Rekonstruktion der Verläufe und Wandlungsprozesse von einzelnen "Fällen" und ihrer Verflechtungen mit anderen "Fällen" die Chancen für eine wirklich empirisch geerdete Theorieentwicklung. URN: http://nbn-resolving.de/urn:nbn:de:0114-fqs1602139

  7. Rezension zu: Elisabeth Tuider, Martin Dannecker: Das Recht auf Vielfalt. Aufgaben und Herausforderungen sexueller Bildung. Göttingen: Wallstein Verlag 2016.

    Directory of Open Access Journals (Sweden)

    Marcus Felix

    2017-06-01

    Full Text Available Sexuelle Bildung ist und war schon immer eine umkämpfte Disziplin der Pädagogik. Dabei ist die aktuelle Erregung um diese als Reaktion auf die fortschreitende Pluralisierung von Lebensentwürfen und die Liberalisierung von Normen und Werten zu verstehen, auf die eine diversitätsbewusste Sexualpädagogik in ihren Konzepten Bezug nimmt, die aber von ultrakonservativen, fundamentalistischen und rechtsradikalen Gruppierungen angegriffen werden. Diese Gemengelage nehmen die Autor_innen zum Anlass, um ausgewählte in der Kritik stehende Inhalte einer sexualfreundlichen Bildung zu besprechen und die Argumentationsmuster der Kritiker_innen zu verdeutlichen. Damit trägt der Band zur (Re-Kontextualisierung und Versachlichung einer hochemotionalen Auseinandersetzung über sexuelle Bildung bei.

  8. Rezension zu: Isabelle Stauffer: Weibliche Dandys, blickmächtige Femmes fragiles. Köln u.a.: Böhlau Verlag 2008

    Directory of Open Access Journals (Sweden)

    Jens F. Heiderich

    2009-07-01

    Full Text Available Stauffer legt eine hervorragende Studie zu ironischen Inszenierungen geschlechtsspezifischer Topoi vor. Literaturgeschichtlich präzise werden die Geschlechterverwirrungen im Fin de Siècle unter besonderer Berücksichtigung der Werke Annette Kolbs und Franziska zu Reventlows kontextualisiert und mit Hilfe eines eigens entwickelten methodologischen Ansatzes analysiert. Überzeugend kann Stauffer darlegen, dass den Ironisierungen der Femme fragile und der Femme fatale, des Décadents und des Dandy die Funktion zukommt, Geschlecht als Konstruktion zu enthüllen. Avant la lettre werde dadurch Judith Butlers Theorie der subversiven Performativität bestätigt. Für die Beschreibung der damit verbundenen Konstruktionsverfahren hält Stauffer allerdings den Begriff der Ironie für treffender als den von Butler u. a. favorisierten Begriff der Parodie.

  9. Rezension zu: Heike Brabandt, Bettina Roß, Susanne Zwingel (Hg.: Mehrheit am Rand? Wiesbaden: VS Verlag für Sozialwissenschaften 2008

    Directory of Open Access Journals (Sweden)

    Katja Strobel

    2009-03-01

    Full Text Available Im vorliegenden Sammelband werden aktuelle Diskussionen zu den Themen Globalierung, Exklusion, Geschlechterverhältnisse und Ethnizität zusammengeführt. Vor allem noch wenig rezipierte postkoloniale Ansätze werden berücksichtigt. Das breite Spektrum an Themen und die Vielfalt an Ansätzen, die von empirischen Studien zur Makroökonomik über historische Analysen bis hin zu detaillierten Fallstudien reicht, überzeugt. Der Anspruch, zum Thema globale Ungleichheit Beiträge aus den zentralen sozialwissenschaftlichen Feldern sinnvoll zusammenzuführen, wird eingelöst.

  10. Spiral Countercurrent Chromatography

    Science.gov (United States)

    Ito, Yoichiro; Knight, Martha; Finn, Thomas M.

    2013-01-01

    For many years, high-speed countercurrent chromatography conducted in open tubing coils has been widely used for the separation of natural and synthetic compounds. In this method, the retention of the stationary phase is solely provided by the Archimedean screw effect by rotating the coiled column in the centrifugal force field. However, the system fails to retain enough of the stationary phase for polar solvent systems such as the aqueous–aqueous polymer phase systems. To address this problem, the geometry of the coiled channel was modified to a spiral configuration so that the system could utilize the radially acting centrifugal force. This successfully improved the retention of the stationary phase. Two different types of spiral columns were fabricated: the spiral disk assembly, made by stacking multiple plastic disks with single or four interwoven spiral channels connected in series, and the spiral tube assembly, made by inserting the tetrafluoroethylene tubing into a spiral frame (spiral tube support). The capabilities of these column assemblies were successfully demonstrated by separations of peptides and proteins with polar two-phase solvent systems whose stationary phases had not been well retained in the earlier multilayer coil separation column for high-speed countercurrent chromatography. PMID:23833207

  11. L’eau convoitée de la Guyane vénézuélienne1

    Directory of Open Access Journals (Sweden)

    Anne Pene-Annette

    2004-12-01

    Full Text Available La Guyane vénézuélienne, région dont la planification est contrôlée par l’Etat, joue un rôle majeur dans le développement économique du pays. Le potentiel hydrologique du fleuve Caroni est convoité pour les usages hydroélectriques, industriels et urbains qui se concentrent dans le pôle d’industrie lourde de Ciudad Guayana. La planification dirigiste, qui se reflète aussi dans la gestion du « pétrole bleu », est en concurrence avec la politique de décentralisation.

  12. Fluchen und Flamen, um ein Mann zu sein? Die Verknüpfung von Geschlecht und Sprache durch Vorurteile

    Directory of Open Access Journals (Sweden)

    Nicola Döring

    2001-11-01

    Full Text Available Die hier besprochene Monographie der Linguistin Anja Gottburgsen beschäftigt sich theoretisch und empirisch mit sprachbezogenen Geschlechterstereotypen, also mit unseren Vorstellungen darüber, ob und wie Männer und Frauen jeweils in spezifischer und distinkter Weise sprachlich kommunizieren. Im Theorieteil der Arbeit wird der aktuelle Stand der soziolinguistischen und sozialpsychologischen Diskussion zu Geschlecht, Sprachverhalten und Stereotypisierung kritisch referiert und die Bedeutung von sprachbezogenen Geschlechterstereotypen erläutert. Im empirischen Teil berichtet die Autorin die Ergebnisse von drei Fragebogen-Studien mit deutschsprachigen Studierenden. Es zeigt sich, dass bei Studierenden unabhängig von Geschlecht und Geschlechtsrollenorientierung stereotype Vorstellungen über das sprachliche Verhalten von Männern und Frauen verbreitet sind.

  13. Zu entwicklungstendenzen im satzbau der Deutschen sprache der gegenwart unter besonderer Berücksichtigung der ausrahmung

    Directory of Open Access Journals (Sweden)

    Jasna Makovec

    1983-01-01

    Full Text Available Die vorliegende Arbeit wurde durch das Bestreben motiviert, weitere Ergänzungen zum Phänornen "Ausrahrnung in der modernen deutschen Schriftsprache der Gegenwart" zu leisten. Der Ausgangspunkt dieses "fakultativ" geregelten Bereiches ist die Annahme bzw. die Arbeitshypothese, daß sich linguistische und außerlinguistische Faktoren einander zuordnen lassen. Das bedeutet, daß die Linearität innerhalb des Kornrnunikationspro­ zesses auch einige wichtige kommunikative Funktionen auszuüben hat, und daß die kontextuellen Modifikationen der Stellungsva­ rianten bei gleichern Denotatsbezug unter diesern Blickwinkel untersucht werden müssen, und nicht gleich als stilistische Varianten qualifiziert werden.

  14. Rezension zu: Nicole C. Karafyllis, Gotling Ulshöfer: Sexualized Brains. Cambridge u.a.: MIT Press 2008

    Directory of Open Access Journals (Sweden)

    Sigrid Schmitz

    2009-07-01

    Full Text Available In der Auseinandersetzung um geschlechtliche Normierungen spielt die moderne Hirnforschung eine wirkmächtige und inzwischen disziplinenübergreifende Rolle. Nicole Karafyllis und Gotlind Ulshöfer stellen in diesem Sammelband mit Beiträgen aus Neurowissenschaft, Psychologie, Soziologie, Kulturwissenschaft und Philosophie ein multiperspektivisches Diskursfeld zu der Frage vor, ob die Emotionale Intelligenz (EI als erstrebenswerte Fähigkeit die klassische Kodierung in männliche Rationalität bzw. weibliche Emotionalität dekonstruieren und ein emanzipatorisches Potential entgegen gesellschaftlichen Hierarchisierungen entfalten kann. Viele Aspekte, Verbindungen zwischen den Diskurssträngen und wechselseitige Beeinflussungen, aber auch disziplinäre Eingrenzungen werden sichtbar, die eine kritische Fortführung des hier angestoßenen Dialogs notwendig machen.

  15. Drei Avantgarde-Strömungen des heutigen US-Geisteslebens – und ihre Beziehung zu Europa

    Directory of Open Access Journals (Sweden)

    Roland Benedikter

    2006-12-01

    Full Text Available Zusammenfassung: Die heutigen USA gelten vielen als Vorreiter auf dem Weg zur integrativen Erneuerung von Wissenschafts- und Erkenntnisparadigmen. Dies vor allem im Bereich der traditionellen Kern- und Grundlagen-Wissenschaft der neuzeitlichen Universität: der Philosophie und der historisch aus ihr erwachsenen Psychologien. Seit einigen Jahren ist in den USA in der Tat eine Entwicklung im Gang, welche die Einseitigkeiten des nominalistisch-subjektivistischen Paradigmas der „Postmoderne“, welches aus ideengeschichtlicher Sicht die Epoche zwischen 1979 und 2001 geprägt hat, um einen neuen geistigen Objektivismus ausgleichen und beide zu einem neuen, „subjektiv-objektiven“ Paradigma integrieren will. Diese Entwicklung findet ihren Ausdruck in drei exemplarischen Avantgarde-Strömungen, die im vorliegenden Beitragvorgestellt sowie auf Charakteristiken und Wechselbeziehungen untersucht werden. Dabei erweist sich, dass die heutige ideengeschichtliche Avantgarde der USA in Kernterminologie, historischer Kontinuität und Ausrichtung stark pazifisch-asiatisch, aber noch zu wenig atlantisch-europäisch geprägt ist. Das scheint mit ein Grund dafür zu sein, warum diese Avantgarde-Ansätze trotz ihres hochwertigen Anregungs- und Innovations-Potentials im Hinblick auf ein ganzheitlichen Wissenschafts-Paradigma für das 21. Jahrhundert noch unübersehbare Schwierigkeiten haben, den atlantisch-europäisch geprägten Hauptstrom des Geistes-, Kultur- und politisch-sozialen Lebens ihrer Gesellschaft zu erreichen. Es zeigt sich, dass der innere Ausgleich zwischen pazifischen und atlantischen Ideen-Einflüssen eine der zentralen Herausforderungen für diese Avantgarde-Strömungen, aber darüber hinaus im Spiegelverhältnis auch für das europäische Kultur- und Gesellschafts-Paradigma sowie für die Entwicklung der integralen Bewegungen auf Weltebene insgesamt ist. Three avant-garde currents within the contemporary intellectual life in the United States

  16. Alle Wege führen nach Rom: Trifft dies auch für die Veränderungsmessung zu?

    DEFF Research Database (Denmark)

    Boecker, Maren; Volz-Sidiropoulou, Eftychia; Nielsen, Tine

    Die Erfassung von Veränderungen mithilfe von Fragebögen spielt eine wichtige Rolle in der klinischen Praxis und Forschung. Ziel dieser Studie war es zu untersuchen, ob im Rahmen der Veränderungsmessung die verwendete Datenanalysemethode einen Einfluss auf die Studienergebnisse hat. Insbesondere w...

  17. Nachtraege zu den „Tertiaerschichten auf Java.” 1ter Nachtrag: Mollusken. Nach Sammlungen der Indischen Bergbeamten, Hunghuhn’s und Reinwardt’s

    NARCIS (Netherlands)

    Martin, K.

    1881-01-01

    Durch Vermittelung Verbeek’s erhielt ich eine grosse Reihe von Versteinerungen, welche von Bergbeamten Indiens (hauptsächlich von R. Everwijn und P. van Dijk) gesammelt waren und sich bis vor Kurzem, ohne näher untersucht zu sein, in Batavia befanden ¹). Diese Sammlung umfasste neben vielem

  18. [Antjekathrin Grassmann. Zu den wirtschaftlichen und persönlichen Beziehungen Lübecks ins Baltikum im Lichte einer unbeachteten mittelalterlichen Quellegruppe]/ Dennis Hortmuth

    Index Scriptorium Estoniae

    Hormuth, Dennis

    2012-01-01

    Arvustus: Antjekathrin Grassmann. Zu den wirtschaftlichen und persönlichen Beziehungen Lübecks ins Baltikum im Lichte einer unbeachteten mittelalterlichen Quellegruppe. In: Leder ist Brot. Beiträge zur norddeutschen Landes- und Archivgeseten. Schwerin 2011. S. 125-135

  19. Declining price difference from gas to coal provides for a new situation in the electricity market; Sinkende Preisdifferenz von Gas zu Kohle sorgt fuer neue Lage am Strommarkt

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2017-01-15

    The price difference (spread) between power coal and natural gas has increased sharply since 1999. This gap reached its peak in 2013 with 171 Euro/t SKE. In many cases, higher CO{sub 2} prices were required to promote gas use in the electricity sector. However, it has now become clear that falling gas prices lead to a growing competitiveness of natural gas. Interventions in the EU-ETS or at the national level with the aim to promote gas use in the electricity sector do not make sense and would merely reduce the competitive pressure on the gas producers and lead them to become a monopoly rents. [German] Der Preisabstand (Spread) zwischen Kraftwerkskohle und Erdgas stieg seit 1999 stark an. Dieser Gap erreichte in 2013 mit 171 Euro/t SKE seinen Hoechststand. Vielfach wurden hoehere CO{sub 2}-Preise gefordert, um den Gaseinsatz im Stromsektor zu foerdern. Doch inzwischen zeigt sich, dass fallende Gaspreise zu einer wachsenden Wettbewerbsfaehigkeit von Erdgas fuehren. Interventionen in das EU-ETS oder auf nationaler Ebene mit dem Ziel, den Gaseinsatz im Stromsektor zu foerdern, machen daher keinen Sinn und wuerden lediglich den Wettbewerbsdruck auf die Gasproduzenten mindern sowie fuer diese zu einer Monopolrente fuehren.

  20. Multiplex gas chromatography

    Science.gov (United States)

    Valentin, Jose R.

    1990-01-01

    The principles of the multiplex gas chromatography (GC) technique, which is a possible candidate for chemical analysis of planetary atmospheres, are discussed. Particular attention is given to the chemical modulators developed by present investigators for multiplex GC, namely, the thermal-desorption, thermal-decomposition, and catalytic modulators, as well as to mechanical modulators. The basic technique of multiplex GC using chemical modulators and a mechanical modulator is demonstrated. It is shown that, with the chemical modulators, only one gas stream consisting of the carrier in combination with the components is being analyzed, resulting in a simplified instrument that requires relatively few consumables. The mechanical modulator demonstrated a direct application of multiplex GC for the analysis of gases in atmosphere of Titan at very low pressures.

  1. Dielectrokinetic chromatography devices

    Science.gov (United States)

    Chirica, Gabriela S; Fiechtner, Gregory J; Singh, Anup K

    2014-12-16

    Disclosed herein are methods and devices for dielectrokinetic chromatography. As disclosed, the devices comprise microchannels having at least one perturber which produces a non-uniformity in a field spanning the width of the microchannel. The interaction of the field non-uniformity with a perturber produces a secondary flow which competes with a primary flow. By decreasing the size of the perturber the secondary flow becomes significant for particles/analytes in the nanometer-size range. Depending on the nature of a particle/analyte present in the fluid and its interaction with the primary flow and the secondary flow, the analyte may be retained or redirected. The composition of the primary flow can be varied to affect the magnitude of primary and/or secondary flows on the particles/analytes and thereby separate and concentrate it from other particles/analytes.

  2. Determination of radiochemical purity using gas chromatography

    International Nuclear Information System (INIS)

    1975-01-01

    The concepts of chromatography, gas chromatography, activity, radiochemical impurity are defined; the procedure of the application of gas chromatography for detecting radiochemical purity of substances is standardized. (E.F.)

  3. Enantioseparations in counter-current chromatography and centrifugal partition chromatography.

    Science.gov (United States)

    Foucault, A P

    2001-01-12

    Examples of chiral separations in counter-current chromatography (CCC) and centrifugal partition chromatography (CPC) are not numerous, due to the difficulty of finding chiral selectors highly selective in the liquid phase as well as a combination of solvents that does not destroy the selectivity and retains the capacity to elute chiral isomers of interest. New ideas and new chiral selectors generally come from other separation techniques, as will be highlighted in this review.

  4. Mehr Last als Lust – Ein weiterer Sammelband zu „(Frauen-Körper“ More Encumbrance than Enjoyment

    Directory of Open Access Journals (Sweden)

    Paula-Irene Villa

    2006-11-01

    Full Text Available Dieser Band vereint acht heterogene Aufsätze zu „Lust und Last am eigenen Körper“ von Frauen. Alle Beiträge gehören zum Spektrum psychologischer Reflexion; die Themen reichen dabei von Sport über Kochen und Essen bis Schönheit, die Zugänge von Psychoanalyse über experimentelle Psychologie bis hin zu freier Assoziation auf der Grundlage klinisch-praktischer Arbeit. Ein roter Faden durch die Beiträge ist nicht erkennbar und so wirkt die Vielfalt beliebig. So unterschiedlich die Beiträge sind, so unterschiedlich ist auch ihre Qualität. Leider kommen einige Artikel über die unkritische Zusammenstellung anderer Arbeiten nicht hinaus, die meisten nehmen kaum Bezug auf das inzwischen breite und institutionalisierte Feld der (sozial- und kulturwissenschaftlichen Körperforschung. Doch haben manche Aufsätze interessante empirische Einblicke zu bieten, die zur weiteren Reflexion anregen, und einige wenige Beiträge sind so differenziert, dass sich mit ihnen weiterdenken und -forschen lässt. Insgesamt bereitet die Lektüre des Buches weder ‚Einsteiger/-innen‘ noch ‚Fortgeschrittenen‘ der wissenschaftlichen Beschäftigung mit (Frauen-Körpern Lust, sondern erweist sich – bis auf Ausnahmen, die allerdings auch in ausführlicherer Form anderenorts publiziert wurden – eher als Last. Ärgerlich und alles andere als argumentativ überzeugend ist darüber hinaus das in manchen Texten enthaltene, mehr oder minder explizit artikulierte, ‚Feminismus- bzw. Frauenforschungs-Bashing‘.This volume joins together eight heterogeneous essays by women under the theme “enjoyment and encumbrance of the body.” All of the contributions belong within the spectrum of psychological reflection; the themes range from sports, cooking and eating, and beauty, to the process of psychoanalysis, experimental psychology, and free-association based on clinical practice. The thread throughout the contributions remains indiscernible and thus the

  5. Centrifugal precipitation chromatography

    Science.gov (United States)

    Ito, Yoichiro; Lin, Qi

    2009-01-01

    Centrifugal precipitation chromatography separates analytes according their solubility in ammonium sulfate (AS) solution and other precipitants. The separation column is made from a pair of long spiral channels partitioned with a semipermeable membrane. In a typical separation, concentrated ammonium sulfate is eluted through one channel while water is eluted through the other channel in the opposite direction. The countercurrent process forms an exponential AS concentration gradient through the water channel. Consequently, protein samples injected into the water channel is subjected to a steadily increasing AS concentration and at the critical AS concentration they are precipitated and deposited in the channel bed by the centrifugal force. Then the chromatographic separation is started by gradually reducing the AS concentration in the AS channel which lowers the AS gradient concentration in the water channel. This results in dissolution of deposited proteins which are again precipitated at an advanced critical point as they move through the channel. Consequently, proteins repeat precipitation and dissolution through a long channel and finally eluted out from the column in the order of their solubility in the AS solution. The present method has been successfully applied to a number of analytes including human serum proteins, recombinant ketosteroid isomerase, carotenoid cleavage enzymes, plasmid DNA, polysaccharide, polymerized pigments, PEG-protein conjugates, etc. The method is capable to single out the target species of proteins by affinity ligand or immunoaffinity separation. PMID:19541553

  6. Microemulsion Electrokinetic Chromatography.

    Science.gov (United States)

    Buchberger, Wolfgang

    2016-01-01

    Microemulsion electrokinetic chromatography (MEEKC) is a special mode of capillary electrophoresis employing a microemulsion as carrier electrolyte. Analytes may partition between the aqueous phase of the microemulsion and its oil droplets which act as a pseudostationary phase. The technique is well suited for the separation of neutral species, in which case charged oil droplets (obtained by addition of an anionic or cationic surfactant) are present. A single set of separation parameters may be sufficient for separation of a wide range of analytes belonging to quite different chemical classes. Fine-tuning of resolution and analysis time may be achieved by addition of organic solvents, by changes in the nature of the surfactants (and cosurfactants) used to stabilize the microemulsion, or by various additives that may undergo some additional interactions with the analytes. Besides the separation of neutral analytes (which may be the most important application area of MEEKC), it can also be employed for cationic and/or anionic species. In this chapter, MEEKC conditions are summarized that have proven their reliability for routine analysis. Furthermore, the mechanisms encountered in MEEKC allow an efficient on-capillary preconcentration of analytes, so that the problem of poor concentration sensitivity of ultraviolet absorbance detection is circumvented.

  7. Introduction to modern liquid chromatography

    National Research Council Canada - National Science Library

    Snyder, Lloyd R; Kirkland, J. J; Dolan, John W

    2010-01-01

    "High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample...

  8. Gas chromatography: mass selective detector

    International Nuclear Information System (INIS)

    Lapinskas, R.

    1988-01-01

    The mechanism of mass spectrometry technique directed for detecting molecular structures is described, with some considerations about its operational features. This mass spectrometer is used as a gas chromatography detector. (author)

  9. Calcium-sensitive immunoaffinity chromatography

    DEFF Research Database (Denmark)

    Henriksen, Maiken L; Lindhardt Madsen, Kirstine; Skjoedt, Karsten

    2014-01-01

    Immunoaffinity chromatography is a powerful fractionation technique that has become indispensable for protein purification and characterization. However, it is difficult to retrieve bound proteins without using harsh or denaturing elution conditions, and the purification of scarce antigens...... to homogeneity may be impossible due to contamination with abundant antigens. In this study, we purified the scarce, complement-associated plasma protein complex, collectin LK (CL-LK, complex of collectin liver 1 and kidney 1), by immunoaffinity chromatography using a calcium-sensitive anti-collectin-kidney-1 m...... chromatography was superior to the traditional immunoaffinity chromatographies and resulted in a nine-fold improvement of the purification factor. The technique is applicable for the purification of proteins in complex mixtures by single-step fractionation without the denaturation of eluted antigens...

  10. Flow Rates in Liquid Chromatography, Gas Chromatography and Supercritical Fluid Chromatography: A Tool for Optimization

    Directory of Open Access Journals (Sweden)

    Joris Meurs

    2016-08-01

    Full Text Available This paper aimed to develop a standalone application for optimizing flow rates in liquid chromatography (LC, gas chromatography (GC and supercritical fluid chromatography (SFC. To do so, Van Deemter’s equation, Knox’ equation and Golay’s equation were implemented in a MATLAB script and subsequently a graphical user interface (GUI was created. The application will show the optimal flow rate or linear velocity and the corresponding plate height for the set input parameters. Furthermore, a plot will be shown in which the plate height is plotted against the linear flow velocity. Hence, this application will give optimized flow rates for any set conditions with minimal effort.

  11. Intermediale Bezugnahme und mediale Materialität Eine Replik auf Beatrice Nickels Aufsatz »Poetry gets into the city. Beobachtungen zu intermedialen Dichtungen in der Stadt«

    Directory of Open Access Journals (Sweden)

    Johanne Mohs

    2012-05-01

    Full Text Available In ihrer Replik auf den Beitrag von Beatrice Nickel weitet Johanne Mohs die Perspektive auf ›Gedichtobjekte‹ im städtischen Raum nach 1945 aus. Eingangs differenziert sie den Begriff der Intermedialität, um daraufhin die von Beatrice Nickel angeführten Beispiele analytisch zu spezifizieren und interpretatorisch zu vertiefen. Letztlich folgt ihre Lesart den Avantgardekonzepten des frühen 20. Jahrhunderts, um das von den Künstlern problematisierte Wechselverhältnis von ›Kunst‹ und ›Leben‹ zu beleuchten.

  12. Intermediale Bezugnahme und mediale Materialität. Eine Replik auf Beatrice Nickels Aufsatz »Poetry gets into the city: Beobachtungen zu intermedialen Dichtungen in der Stadt«

    Directory of Open Access Journals (Sweden)

    Johanne Mohs

    2012-05-01

    Full Text Available In ihrer Replik auf den Beitrag von Beatrice Nickel weitet Johanne Mohs die Perspektive auf ›Gedichtobjekte‹ im städtischen Raum nach 1945 aus. Eingangs differenziert sie den Begriff der Intermedialität, um daraufhin die von Beatrice Nickel angeführten Beispiele analytisch zu spezifizieren und interpretatorisch zu vertiefen. Letztlich folgt ihre Lesart den Avantgardekonzepten des frühen 20. Jahrhunderts, um das von den Künstlern problematisierte Wechselverhältnis von ›Kunst‹ und ›Leben‹ zu beleuchten.

  13. Tutorium Quantenfeldtheorie was Sie schon immer über QFT wissen wollten, aber bisher nicht zu fragen wagten

    CERN Document Server

    Edelhäuser, Lisa

    2016-01-01

    Dieses Buch richtet sich an alle, die sich schon immer gefragt haben, wie die kanonische Quantisierung, die LSZ-Reduktionsformel, Pfadintegrale, Feynman-Graphen und die Renormierung miteinander zusammenhängen. Als locker geschriebene Begleitlektüre zu Vorlesungen über Quantenfeldtheorie oder zum Selbststudium geeignet, gibt sich das Buch gesprächig und liefert Rechentricks und Erklärungen, die für Einsteiger sehr hilfreich sind. Im ersten Teil werden anhand von Skalarfeldern grundlegende Konzepte von der klassischen Feldtheorie bis zur Renormierung eingeführt. Der zweite Teil verallgemeinert diese für Felder mit Spin und legt mit der Einführung des Eichprinzips die Grundlagen für den dritten Teil. Hier werden „Anwendungen auf die reale Welt“ behandelt: Die Quantenelektrodynamik und ihre Renormierung, sowie das Standardmodell der Teilchenphysik und der Higgs-Mechanismus. Durch ausführlich vorgerechnete und in den Text eingebundene Aufgaben eignet sich das Tutorium sowohl zum schnellen Nachschla...

  14. Vulnerabilität. Erläuterungen zu einem Schlüsselbegriff im Denken Judith Butlers

    Directory of Open Access Journals (Sweden)

    Pistrol Florian

    2016-07-01

    Full Text Available Im neueren Denken Judith Butlers nimmt der Begriff der Vulnerabilität eine Schlüsselrolle ein. Kaum eines der vielen Themen, denen sich die US-amerikanische Autorin seit der Jahrtausendwende widmet, kann ohne Kenntnis ihrer Überlegungen zu diesem Terminus angemessen verstanden werden. Der Versuch einer Rekonstruktion ihrer Konzeption von Vulnerabilität steht allerdings vor der Schwierigkeit, dass Butlers Gedanken hierzu über zahlreiche Texte hinweg verstreut sind, wobei bisweilen unterschiedliche Aspekte betont und verschiedene Schwerpunktsetzungen vorgenommen werden. Im vorliegenden Beitrag werden Butlers vulnerabilitätstheoretische Ausführungen in gebündelter Weise dargestellt und interpretiert. Dabei wird (1 die Bedeutsamkeit von Butlers Überlegungen zur Vulnerabilität für ihre Vorstellung von Körperlichkeit aufgewiesen, (2 der Zusammenhang von Vulnerabilität und Anerkennung erläutert und (3 gezeigt, welche ethischen Erwägungen Butler ausgehend von ihrer Anerkennungskonzeption und ihrem Denken der Vulnerabilität anstellt und inwiefern Vulnerabilität und politische Handlungsfähigkeit einander nicht ausschließen. Abschließend wird (4 Butlers Vulnerabilitätsverständnis mit einigen kritischen Rückfragen konfrontiert.

  15. Rezension zu: Sheila Jeffreys: The Industrial Vagina. The Political Economy of the Global Sex Trade. London: Routledge 2008.

    Directory of Open Access Journals (Sweden)

    Susanne Hofmann

    2010-02-01

    Full Text Available Sheila Jeffreys analysiert die Prozesse, die zu einer Industrialisierung und Globalisierung von Prostitution im späten 20. und 21. Jahrhundert geführt haben. Hauptverantwortlich für die gegenwärtige Diskursverschiebung um Prostitution ist für Jeffreys zum einen die sexuelle Revolution der 1970er Jahre und zum anderen die massive Finanzierung von Unterstützer/-innen der Sexarbeiterinnen zur HIV-Prävention in den 1980ern. Jeffreys legt wie in früheren Arbeiten ihre radikal-feministische Position dar und fordert eine Abschaffung der Prostitution. In ihrer Abrechnung mit dem liberalen feministischen Diskurs, für den eine Unterstützung von Sexarbeiterinnen prioritär ist, ignoriert sie die Widersprüchlichkeiten und Komplexitäten der gelebten Realitäten. An vielen Stellen ihres Buches bekräftigt Jeffreys stereotypische Männlichkeitsvorstellungen, was durch einen Blick über den Tellerrand des ihr vertrauten wissenschaftlichen Bezugsrahmens hätte vermieden werden können.

  16. Gerechtigkeit, ethische Subjektivität und Alterität. Zu den normativen Implikationen der Philosophie von Emmanuel Levinas

    Directory of Open Access Journals (Sweden)

    Seitz Sergej

    2016-07-01

    Full Text Available Obwohl seit einiger Zeit ein verstärktes Forschungsinteresse an Emmanuel Levinas’ Denken des Politischen, verbunden mit einer genaueren Inblicknahme der zentralen Begriffe des Dritten und der Gerechtigkeit aufgekommen ist, blieb bislang unklar, inwiefern aus Levinas’ intrinsisch ‚ethischem‘ Denken in konkreterer Weise normative Konsequenzen zu ziehen sind. Diesem Desiderat versucht der vorliegende Beitrag nachzukommen. Das Levinas’sche Gerechtigkeitsdenken wird dabei so eingeführt, dass es zugleich als alternativer Entwurf und als produktive kritische Provokation für gegenwärtige Gerechtigkeitstheorien lesbar wird. Ausgehend von einer Rekonstruktion des alteritären Verständnisses ethischer Subjektivität, wie es Levinas insbesondere in Jenseits des Seins erarbeitet, wird ausgelotet, wie tragfähig Levinas’ ethische Konzeption plural alteritär durchfurchter Subjektivität für normative Gerechtigkeitsfragen ist. Aus einer Reflexion auf den Begriff des ‚Dritten‘ werden einige konkrete Konsequenzen für das Denken gerechter Institutionen gezogen.

  17. Rezension zu: Mary Pepchinski: Feminist Space. Exhibitions and Discourses between Philadelphia and Berlin 1865–1912. Weimar: VDG Verlag und Datenbank für Geisteswissenschaften 2007.

    Directory of Open Access Journals (Sweden)

    Monika Kaiser

    2009-10-01

    Full Text Available Mary Pepchinski widmet ihre Untersuchung temporären Ausstellungen, die im Zeitraum von 1865 bis 1912 im deutschsprachigen Raum und in den USA entstanden sind und die zum Ziel hatten, in der bürgerlichen Öffentlichkeit Räume für feministische Anliegen und Diskussionen zu schaffen. In diesen um 1900 neu entstehenden Räumen der weiblichen Kultur spiegeln sich die feministischen Diskurse der Epoche und fließen in die von den Akteurinnen des gehobenen Bürgertums geschaffenen, charakteristischen Raumschöpfungen ein. Die Autorin stellt bislang unbekanntes Quellenmaterial vor und eröffnet durch ihren interdisziplinären Blick den Zugang zu einem vernachlässigten, da bislang unbekannten Teil der Kulturgeschichte der Moderne.

  18. Wybrane Aspekty Zużywania Się Połączeń Wpustowych I Wielowypustowych W Procesie Eksploatacji

    Directory of Open Access Journals (Sweden)

    Gębura Andrzej

    2014-12-01

    Full Text Available W artykule przedstawiono wybrane wady połączeń wielowypustowych: mimośrodowości, przekoszenia, zwiększonych luzów. Przedstawiono znaczenie tych wad dla poprawności funkcjonowania całego zespołu napędowego. Zaprezentowano swoisty katalog tych wad w formie: wizualnego zużycia, pomiarów mechanicznych płaszczyzn przyporu, opisu matematycznego przebiegów dynamiki ruchu takiego połączenia, zobrazowań dla metod FAM-C i FDM-A. Zaznaczono skutki długotrwałej eksploatacji połączeń wielowypustowych o nadmiernym zużyciu dla bezpieczeństwa latania

  19. Die Übung an sich selbst außerhalb der Ästhetik: das Gegenmittel zu Instrumentalisierungen der Kultur.

    Directory of Open Access Journals (Sweden)

    Stanislas d’Ornano

    2005-10-01

    Full Text Available In heutiger Zeit den politischen Status der Kultur und der Kunst zu untersuchen setzt voraus, dass man ebenfalls drei Aspekte unserer Epoche in Betracht zieht. Wir haben die Orientierung verloren aufgrund des Widerspruchs zwischen der Vielfalt der Wege (Theorien, Lebensstile, Kunstrichtungen einerseits und der Vorstellung der Erreichung eines Ziels (das Ende der Geschichte, die vollkommene Gesellschaft andererseits. Wir erleben täglich die Situation einer zweifachen Instrumentalisierung, ...

  20. Essay (with abstract in English Drei Avantgarde-Strömungen des heutigen US-Geisteslebens – und ihre Beziehung zu Europa

    Directory of Open Access Journals (Sweden)

    Roland Benedikter

    2006-12-01

    Full Text Available Die heutigen USA gelten vielen als Vorreiter auf dem Weg zur integrativen Erneuerung von Wissenschafts- und Erkenntnisparadigmen. Dies vor allem im Bereich der traditionellen Kern- und Grundlagen-Wissenschaft der neuzeitlichen Universität: der Philosophie und der historisch aus ihr erwachsenen Psychologien. Seit einigen Jahren ist in den USA in der Tat eine Entwicklung im Gang, welche die Einseitigkeiten des nominalistisch-subjektivistischen Paradigmas der „Postmoderne“, welches aus ideengeschichtlicher Sicht die Epoche zwischen 1979 und 2001 geprägt hat, um einen neuen geistigen Objektivismus ausgleichen und beide zu einem neuen, „subjektiv-objektiven“ Paradigma integrieren will. Diese Entwicklung findet ihren Ausdruck in drei exemplarischen Avantgarde-Strömungen, die im vorliegenden Beitrag vorgestellt sowie auf Charakteristiken und Wechselbeziehungen untersucht werden. Dabei erweist sich, dass die heutige ideengeschichtliche Avantgarde der USA in Kernterminologie, historischer Kontinuität und Ausrichtung stark pazifisch-asiatisch, aber noch zu wenig atlantisch-europäisch geprägt ist. Das scheint mit ein Grund dafür zu sein, warum diese Avantgarde-Ansätze trotz ihres hochwertigen Anregungs- und Innovations-Potentials im Hinblick auf ein ganzheitliches Wissenschafts-Paradigma für das 21. Jahrhundert noch unübersehbare Schwierigkeiten haben, den atlantisch-europäisch geprägten Hauptstrom des Geistes-, Kultur- und politisch-sozialen Lebens ihrer Gesellschaft zu erreichen. Es zeigt sich, dass der innere Ausgleich zwischen pazifischen und atlantischen Ideen-Einflüssen eine der zentralen Herausforderungen für diese Avantgarde-Strömungen, aber darüber hinaus im Spiegelverhältnis auch für das europäische Kultur- und Gesellschafts-Paradigma sowie für die Entwicklung der integralen Bewegungen auf Weltebene insgesamt ist.

  1. Das Re-entry von Kritik: Assemblageforschung nach der Kritik an der Kritik. Kommentar zu Alexa Färbers „Potenziale freisetzen“

    Directory of Open Access Journals (Sweden)

    Hanna Göbel

    2014-05-01

    Full Text Available Der Kommentar zum Beitrag von Alexa Färber diskutiert eine Vermengung von drei unterschiedlichen Kritikbegriffen sowie Orten von Kritik in der aktuellen Debatte um die Assemblageforschung im Feld der Urban Studies. Ich rekonstruiere und unterscheide zwischen einem Kritikbegriff als theoretischer Norm, wie er von der (neo-marxistischen Stadt- und Raumforschung vertreten wird, und der ontologischen Kritik dieser Norm seitens der Assemblageforscher_innen. Alexa Färbers Beitrag zeigt drittens exemplarisch eine sich einschleichende normative Ebene in der Assemblageforschung auf, die bislang als Kritikform unterbelichtet geblieben ist. Diese wird als Wiedereinführung (re-entry von empirisch vollzogener Kritik in die Assemblageforschung bezeichnet. Damit ist eine theoretische Neupositionierung von Kritik ‚innerhalb’ einer Assemblage gemeint, indem Kritik nicht mehr als theoretische Norm ‚außerhalb’ zu verorten ist. In diesem Kommentar plädiere ich dafür, in der Assemblageforschung eine differenzierte Auseinandersetzung mit unterschiedlichen Begriffen und Orten von Kritik zu pflegen, um innerhalb der Urban Studies und für interessierte städtische Akteure mit urbaner Expertise (u.a. Architekt_innen, Planer_innen, Künstler_innen, Aktivist_innen, Kultur- und Sozialarbeiter_innen, DIY-Gemeinschaften klarer adressierbar zu sein.

  2. Geschlecht zwischen Körper und Konstruktion: Aktuelle Impulse zu einer langen Debatte Between Bodies and Constructions: Current Takes on a Long-Standing Debate

    Directory of Open Access Journals (Sweden)

    Natascha Gruber

    2002-11-01

    Full Text Available Die mit dem Erscheinen von Judith Butlers Unbehagen der Geschlechter ausgelöste „Essentialismus versus Konstruktivismus“-Debatte um die Frage, wie Materialität und Körper zu denken sei, hält die Geschlechterforschung bis heute in dilemmatischer Spannung. Kann auf die Kategorie Geschlecht überhaupt verzichtet werden? Der Sammelband Verhandlungen des Geschlechts dokumentiert anhand von 15 Beiträgen aus den Bereichen Kulturwissenschaften, Philosophie, Psychoanalyse und Politik die aktuelle Entwicklung der Debatte rund um den an Butler orientierten feministischen Konstruktionsbegriff. Unter Rückgriff auf unterschiedliche Ansätze wird versucht, zwischen „Körper“ und „Diskurs“ zu vermitteln und Materialität neu zu denken.The “essentialism-versus-constructivism” debate which was sparked by Butler’s work, Gender Trouble, a debate centered around the question how to best think about materiality and bodies, continues to be relevant to gender studies to the present day. Is it at all possible to avoid gender as a category? 15 essays from scholars in cultural studies, philosophy, psycho-analysis, and politics document the current development of the debates that were inspired by Butler’s ideas about feminist constructions. Using a variety of different approaches, the authors attempt to mediate between the concepts of “body” and “discourse” and to conceive of materiality in a new way.

  3. Alte Thesen neu gelesen: Perspektiven kritischer Stadtforschung. Kommentar zu Hartmut Häußermann & Walter Siebels „Thesen zur Soziologie der Stadt“

    Directory of Open Access Journals (Sweden)

    Carsten Keller

    2013-07-01

    Full Text Available [Dieser Artikel ist Teil einer Debatte] Der Text „Thesen zur Soziologie der Stadt“ scheint mir besonders in den abschließend formulierten Perspektiven, die Hartmut Häußermann und Walter Siebel unter der Überschrift „Aufgaben einer kritischen Soziologie der Stadt“ formulieren, nach wie vor lesenswert und aktuell zu sein. Drei Perspektiven werden hier aufgezeigt: erstens eine Wissenschafts- und Ideologiekritik, zweitens eine empirisch orientierte Zustandsbeschreibung städtischer Strukturen und von Stadtentwicklung sowie drittens eine Analyse der staatlichen Stadtpolitik. Diese drei abschließenden Perspektiven möchte ich im Folgenden kommentieren. Es wird mithin keine immanente Auseinandersetzung mit dem gesamten Text und weiteren Arbeiten der beiden Autoren angestrebt. Es hätte mich zwar durchaus gereizt, die Entwicklung der Arbeiten von Häußermann und Siebel vor dem Hintergrund dieses frühen, emphatisch formulierten Textes zu reflektieren. Vielleicht auch wegen der persönlichen Bekanntschaft mit Häußermann, den ich als Projekt- und Dissertationsbetreuer an der Humboldt-Universität kennenlernte, erschien mir eine solche Zugangsweise zu den „Thesen“ durchaus naheliegend. [...

  4. Für Forschung und Kultur - Öffentlichkeitswirksame Darstellung bibliothekarischer Weltschätze und bibliothekarischer Wissenschaftsservices am Beispiel der Staatsbibliothek zu Berlin

    Directory of Open Access Journals (Sweden)

    Barbara Schneider-Kempf

    2014-12-01

    Full Text Available Die Öffentlichkeitsarbeit der Staatsbibliothek zu Berlin zielt auf eine wirksame Darstellung ihrer bibliothekarischen Weltschätze und ihrer Serviceangebote für die Wissenschaft ab. Sie ruht auf drei Säulen: kulturelle Breitenbildung für wenig bibliotheksaffine Kreise, allgemeine und breit gefächerte Öffentlichkeitsarbeit sowie Aktionen, die sich an spezielle communities in der Wissenschaft richten. Der Beitrag informiert auch über die erfolgreiche Umsetzung dieser Strategie anhand zahlreicher Beispiele. Er zeigt, wie es der Staatsbibliothek in der Hauptstadt Berlin, in der viele Gedächtnisinstitutionen tätig sind, gelingt, öffentlich präsent zu bleiben und ihr Profil zu vermitteln. The public relations work of the Berlin State Library – Prussian Cultural Heritage aims at effectively presenting its globally significant treasures as well as its services for the scientific world. It is based on three pillars: cultural education for the general public, especially for people who are not naturally close to libraries; a general and wide variety of public relations work; and events which target special communities in science. The paper also presents many examples for the effectual implementation of this strategy. It shows how the State Library, in the capital city of Berlin with its many memory institutions, succeeds in keeping a strong presence and communicating its profile to the public.

  5. Intertextuelle und intramediale Bezüge als Orientierungsangebot – systematische Überlegungen und exemplarische Untersuchungen zu Verweisen auf das Fernsehangebot

    Directory of Open Access Journals (Sweden)

    Ben Bachmair

    2017-07-01

    Full Text Available Auch wenn das Internet sich weder so schnell noch so nachhaltig im Alltag und als Bestandteil von Massenkommunikation etablierte, wie es Ende der neunziger Jahre den Anschein hatte, so zeigt sich am Internet doch, was sich mit der Vernetzungs- und Verfügbarkeitslogik verändern könnte, die dem Internet zu eigen ist. Manuel Castells (2001 mit seiner Analyse des „Informationszeitalters“ und der als instrumentellem Netzwerk organisierten Gesellschaft skizziert die Umrisse, wie sich unsere Gesellschaft mit welcher Dynamik entwickelt. Er spricht von einer „informationeilen Entwicklungsweise“ (ebd., S. 17, die anders als die „agrarische“ oder die „industrielle“ auf das gesellschaftliche Verhältnis der Menschen zur Natur („Produktion“, zu sich selber („Erfahrung“, „kulturelle Identität“ und zu anderen (.Macht“ einwirkt. Einen Aspekt dieser „informationellen Entwicklungsweise“ beschreibt Castells mit dem Hinweis auf „globale Netzwerke der Instrumentalität“, die eine „unüberschaubare Anzahl virtueller Gemeinschaften“ hervorbringt (ebd., S. 23. Dabei konstituiert sich auch eine Art von Subjektivität, die sich im Moment als „zunehmende Distanz zwischen Globalisierung und Identität, zwischen dem Netz und dem Ich“ beschreiben lässt (ebd., S. 24. In dieser Spannung bekommt .Multimedia“ die Funktion einer „symbolischen Umwelt“ (ebd., S. 415.

  6. Benjamin Ortmeyer: Mythos und Pathos statt Logos und Ethos, Zu den Publikationen führender Erziehungswissenschaftler in der NS-Zeit: Eduard Spranger, Herman Nohl, Erich Weniger und Peter Petersen, Weinheim / Basel: Beltz 2009 [Rezension

    OpenAIRE

    Zimmer, Hasko

    2010-01-01

    Rezension von: Benjamin Ortmeyer: Mythos und Pathos statt Logos und Ethos, Zu den Publikationen führender Erziehungswissenschaftler in der NS-Zeit: Eduard Spranger, Herman Nohl, Erich Weniger und Peter Petersen, Weinheim / Basel: Beltz 2009, 606 S.

  7. Nitrogen dioxide - current knowledge on the pollution situation and health effects; Stickstoffdioxid - Kenntnisstand zur Belastungssituation und zu gesundheitlichen Wirkungen

    Energy Technology Data Exchange (ETDEWEB)

    Muecke, H.G. [Umweltbundesamt, Berlin (Germany). Inst. fuer Wasser-, Boden- und Lufthygiene

    1999-07-01

    Feuerstellen (z.B. Gasherde, Gasheizautomaten und Heisswasserbereiter) und das Tabakrauchen sind die NO{sub 2}-Hauptquellen der Luftbelastung in Innenraeumen. NO{sub 2}-Messungen in deutschen Kuechen ergaben mittlere Konzentrationen von bis zu 50 {mu}g/m{sup 3} und Spitzenkonzentrationen von fast 200 {mu}G/m{sup 3}. Die NO{sub 2}-Konzentration in Innenraeumen ohne Stickstoffoxid-Quellen wird von den Aussenluftverhaeltnissen bestimmt und liegt im Durchschnitt zwischen 20 und 30 {mu}G/m{sup 3}. Die ueberarbeiteten NO{sub 2}-Richtwerte der WHO von 1998 sind: 40 {mu}g/m{sup 3} als Jahresmittel und 200 {mu}g/m{sup 3} als Einstundenmittel. Als oxidatives und gering wasserloesliches Reizgas entwickelt NO{sub 2} nach Inhalation seine Hauptwirkung in der Lungenperipherie. Veraenderungen von Lungenfunktionsparametern und Zunahme der bronchialen Reagibilitaet sind die wesentlichen gesundheitlichen Wirkungen. Expositionsstudien zur Belastungssituation zeigen, dass Innenraumquellen zusaetzlich im Mittel bis zu 5 {mu}g NO{sub 2}/m{sup 3} beitragen koennen und dass das Lueftungsverhalten als eine wesentliche Einflussgroesse mitangesehen werden kann. (orig.)

  8. Extraction chromatography of fission products

    International Nuclear Information System (INIS)

    Bonnevie-Svendsen, M.; Goon, K.

    1978-01-01

    Various cases of using extraction chromatography during analysis of fission products are reviewed. The use of the extraction chromatography method is considered while analysing reprocessed products of nuclear fuel for quantitative radiochemical analysis and control of fission product and actinoide separation during extraction and their chemical state in production solutions. The method is used to obtain pure fractions of typical burnup monitors (neodymium, molybdenum, cerium, cesium, europium, lanthanides) during determination of nuclear fuel burnup degree. While studying the nature of nuclear reactions the method is used to separate quickly short-life isotopes, to purify β-radiator fractions before measuring their half-life periods, to enrich isotopes forming with low output during fission. Examples of using extraction chromatography are given to separate long half-life or stable fission products from spent solutions, to control environment object contamination

  9. Capillary gas-solid chromatography

    International Nuclear Information System (INIS)

    Berezkin, V.G.

    1996-01-01

    Modern state of gas adsorption chromatography in open capillary columns has been analyzed. The history of the method development and its role in gas chromatography, ways to construct open adsorptional capillary columns, foundations of the theory of retention and washing of chromatographic regions in gas adsorption capillary columns have been considered. The fields is extensively and for analyzing volatile compounds of different isotopic composition, inorganic and organic gases, volatile organic polar compounds, aqueous solutions of organic compounds. Separation of nuclear-spin isomers and isotopes of hydrogen is the first illustrative example of practical application of the adsorption capillary chromatography. It is shown that duration of protium and deuterium nuclear isomers may be reduced if the column temperature is brought to 47 K

  10. From eco-efficiency in overall sustainable development in enterprises; Von Oekoeffizienz zu nachhaltiger Entwicklung in Unternehmen

    Energy Technology Data Exchange (ETDEWEB)

    Weizsaecker, E.U. von; Stigson, B; Seiler-Hausmann, J D [eds.

    2001-07-01

    Eco-efficiency is achieved when goods and services satisfy human needs, increase the quality of life, at prices which are competitive and when environmental impacts and resource intensity are decreased to a degree that they are in accordance with the expected capacities of the Earth. Eco-efficiency is a management approach that allows enterprises to carry out environmental protection measures from a market-oriented point of view. Eco-efficiency shows that ecology and economy do not contradict each other. On the contrary, in combination, they present a gain for enterprises. The motto is: To produce more with less. The WBCSD and its member enterprises have agreed to make economic growth and sustainable development the guideline for their economic entrepreneurial actions. The WBCSD supports the close co-operation of the economy, government and other organizations, as it is only together that we can help sustainable development to achieve a breakthrough. (orig.) [German] Oekoeffizienz ist dann erreicht, wenn Gueter und Dienstleistungen die menschlichen Beduerfnisse befriedigen, die Lebensqualitaet erhoehen und wettbewerbsfaehige Preise aufweisen, und die oekologischen Auswirkungen und die Ressourcenintensitaet waehrend des Lebenszyklus soweit verringert werden, dass eine Uebereinstimmung mit der voraussichtlichen Belastbarkeit der Erde besteht. Oekoeffizienz steht fuer einen Managementansatz, der Unternehmen erlaubt, Umweltschutzmassnahmen unter Marktgesichtspunkten durchzufuehren. Mit Oekoeffizienz wird zum Ausdruck gebracht, dass Oekonomie und Oekologie sich nicht ausschliessen, sondern in Kombination einen Gewinn fuer Unternehmen darstellen. Das Motto lautet: Mehr mit weniger produzieren. Der WBCSD und seine Mitgliedsunternehmen haben sich darauf verstaendigt, ihr Handeln von den Prinzipien des oekonomischen Wachstums und der nachhaltigen Entwicklung leiten zu lassen. Der WBCSD unterstuetzt die enge Zusammenarbeit von Wirtschaft, Staat und anderen Organisationen

  11. Erictho und die Figuren der Entzweiung – Vorüberlegungen zu einer Poetik der Emergenz in Lucans Bellum civile

    Directory of Open Access Journals (Sweden)

    Alexander Arweiler

    2010-10-01

    Full Text Available Im Ausgang von der Möglichkeit, mit dem Wort figura Gestalten der Handlungsebene, dynamisch gefaßte Gedanken- / Wortgebilde und Erscheinungsweisen von Phänomenen geistiger und materieller Natur zu bezeichnen, läßt sich das Bellum civile als Werk beschreiben, an dessen Grund die Figur der Entzweiung steht, die durch eine Poetik der Emergenz in den Stoff entfaltet wird. Die vorgeschlagene Lektüre des Bellum civile wendet sich damit gegen das verbreitete Verständnis des Textes als eines (politischen, moralischen Kommentars zum Bürgerkrieg und kehrt die Hierarchie um: Der (sekundäre Bürgerkriegsstoff soll die zugrunde liegende Figur der Entzweiung explizieren, wird von dieser aber nicht gedeutet, sondern benutzt. Emergenz tritt als poetische Verhältnisbestimmung an die Stelle chronologischer und kausaler Erklärungsmodelle und behauptet eine bleibende Verbindung von Untergrund und hervortretendem Relief (Ereignis, Wesen, Gestalt. Dementsprechend lassen sich die Gestalten der Handlungsebene (figurae als Ekstasen des Textes fassen, die in einer Verdichtung des Textes aus dessen Oberfläche hervortreten, zugleich gemeinsam mit anderen Textelementen Erscheinungsweisen (figurae der abstrakten Figur der Entzweiung sind. Im sechsten Buch des Bellum civile wird die Ekstase insbesondere im Hervorgehen der Erictho aus der Konstruktion eines raum-zeitlich unmöglichen Thessalien kompositionell konkretisiert und imitationstechnischumgesetzt: Die ovidisch geprägte Begegnungsszenezwischen Sextus Pompeius und der textuell übercodierten Erictho bereitet den Boden für die Auffassung der Hexesowohl als einer Fortschreibung der nach Ovid vergehenden Sibylle als auch als figura (Gestalt und Erscheinungsweise Roms, das sich in einer Trennung von sich selbst gegenübertritt und im (poetischen Kannibalismus die Entzweiung zumVollzug bringt.

  12. Isotopic separation by ion chromatography

    International Nuclear Information System (INIS)

    Albert, M.G.; Barre, Y.; Neige, R.

    1994-01-01

    The isotopic exchange reaction and the isotopic separation factor are first recalled; the principles of ion chromatography applied to lithium isotope separation are then reviewed (displacement chromatography) and the process is modelled in the view of dimensioning and optimizing the industrial process; the various dimensioning parameters are the isotopic separation factor, the isotopic exchange kinetics and the material flow rate. Effects of the resin type and structure are presented. Dimensioning is also affected by physico-chemical and hydraulic parameters. Industrial implementation features are also discussed. 1 fig., 1 tab., 5 refs

  13. Atomic Force Microscope Mediated Chromatography

    Science.gov (United States)

    Anderson, Mark S.

    2013-01-01

    The atomic force microscope (AFM) is used to inject a sample, provide shear-driven liquid flow over a functionalized substrate, and detect separated components. This is demonstrated using lipophilic dyes and normal phase chromatography. A significant reduction in both size and separation time scales is achieved with a 25-micron-length column scale, and one-second separation times. The approach has general applications to trace chemical and microfluidic analysis. The AFM is now a common tool for ultra-microscopy and nanotechnology. It has also been demonstrated to provide a number of microfluidic functions necessary for miniaturized chromatography. These include injection of sub-femtoliter samples, fluidic switching, and sheardriven pumping. The AFM probe tip can be used to selectively remove surface layers for subsequent microchemical analysis using infrared and tip-enhanced Raman spectroscopy. With its ability to image individual atoms, the AFM is a remarkably sensitive detector that can be used to detect separated components. These diverse functional components of microfluidic manipulation have been combined in this work to demonstrate AFM mediated chromatography. AFM mediated chromatography uses channel-less, shear-driven pumping. This is demonstrated with a thin, aluminum oxide substrate and a non-polar solvent system to separate a mixture of lipophilic dyes. In conventional chromatographic terms, this is analogous to thin-layer chromatography using normal phase alumina substrate with sheardriven pumping provided by the AFM tip-cantilever mechanism. The AFM detection of separated components is accomplished by exploiting the variation in the localized friction of the separated components. The AFM tip-cantilever provides the mechanism for producing shear-induced flows and rapid pumping. Shear-driven chromatography (SDC) is a relatively new concept that overcomes the speed and miniaturization limitations of conventional liquid chromatography. SDC is based on a

  14. Metal-organic frameworks in chromatography.

    Science.gov (United States)

    Yusuf, Kareem; Aqel, Ahmad; ALOthman, Zeid

    2014-06-27

    Metal-organic frameworks (MOFs) emerged approximately two decades ago and are the youngest class of porous materials. Despite their short existence, MOFs are finding applications in a variety of fields because of their outstanding chemical and physical properties. This review article focuses on the applications of MOFs in chromatography, including high-performance liquid chromatography (HPLC), gas chromatography (GC), and other chromatographic techniques. The use of MOFs in chromatography has already had a significant impact; however, the utilisation of MOFs in chromatography is still less common than other applications, and the number of MOF materials explored in chromatography applications is limited. Copyright © 2014 Elsevier B.V. All rights reserved.

  15. Selectivity in microemulsion electrokinetic chromatography

    DEFF Research Database (Denmark)

    Pedersen-Bjergaard, S; Gabel-Jensen, Charlotte; Honoré Hansen, S

    2000-01-01

    Microemulsion electrokinetic chromatography (MEEKC) is a most promising separation technique providing good selectivity and high separation efficiency of anionic, cationic as well as neutral solutes. In MEEKC lipophilic organic solvents dispersed as tiny droplets in an aqueous buffer by the use...

  16. Liquid chromatography in migration studies

    International Nuclear Information System (INIS)

    Carlsen, L.; Batsberg, W.

    1982-01-01

    Liquid chromatography ahs been suggested as a facile experimental technique to determine important physico-chemical properties, as permeability, porosity, dispersion-, diffusion-, and sorption charcteristics for geological material as chalk samples. The feasibility of the trechnique as a rapid method to evaluate the possible influence of changes in ground water composition on the migration behavior of radionuclides has been demonstrated

  17. Liquid phase chromatography on microchips

    DEFF Research Database (Denmark)

    Kutter, Jörg Peter

    2012-01-01

    explosive development of, in particular, chromatographic separation systems on microchips, has, however, slowed down in recent years. This review takes a closer, critical look at how liquid phase chromatography has been implemented in miniaturized formats over the past several years, what is important...

  18. Undergraduate Separations Utilizing Flash Chromatography

    Science.gov (United States)

    Horowitz, G.

    2000-02-01

    This article describes the procedures used to carry out four flash chromatography experiments: the isolation of the carotenes, chlorophylls and xanthophylls from a spinach extract; the separation of ß-carotene from tetraphenyl cyclopentadienone; the isolation of (+) and (-) carvone from caraway and spearmint oil; and the purification of benzil from benzoin. Apparatus used is nonbreakable, easy to use, and inexpensive.

  19. Thin-Layer and Paper Chromatography.

    Science.gov (United States)

    Sherma, Joseph; Fried, Bernard

    1984-01-01

    Reviews literature on chromatography examining: books, reviews, student experiments; chromatographic systems, techniques, apparatus; detecting and identification of separated zones; preparative chromatography and radiochromatography; and applications related to specific materials (such as acids, alcohols, amino acids, antibiotics, enzymes, dyes,…

  20. Chromatography: Are We Getting It Right?

    Science.gov (United States)

    Maitland, Pamela D.; Maitland, David P.

    2002-01-01

    Explains the basics of chromatography which is used to demonstrate the separation of plant photosynthetic pigments. Reports the results of an evaluative study that explored textbook errors in explaining how chromatography works. (Contains 13 references.) (Author/YDS)

  1. Stimmen zu Estlands Eurobeitritt

    Index Scriptorium Estoniae

    2011-01-01

    Arvamusi Eesti liitumisest eurotsooniga: Toomas Hendrik Ilves, Andrus Ansip, Juhan Parts, Angela Merkel, Jose Manuel Barroso, Olli Rehn, Paul Krugman Anti Poolamets, Nicolaus Heinen, Edgar Savisaar. Lisatud väljavõtted saksakeelsetest ajakirjadest ja ajalehtedest. Euro kasutuselevõttu seostatakse heaolu ja stabiilsusega, samas peetakse üleminekut uuele vääringule liiga kulukaks ja rutakaks

  2. Comparative determination of phenytoin by spectrophotometry, gas chromatography, liquid chromatography, enzyme immunoassay, and radioimmunoassay

    International Nuclear Information System (INIS)

    Castro, A.; Ibanez, J.; DiCesare, J.L.; Adams, R.F.; Malkus, H.

    1978-01-01

    Sera from patients being treated with phenytoin were analyzed for the drug by spectrophotometry, gas chromatography, radioimmunoasay, enzyme immunoassay, and liquid chromatography. The assay values obtained were intercompared statistically. Enzyme immunoassay and liquid chromatography appear to be attractive alternatives to the more traditional methods of spectrophotometry and gas chromatography. Our radioimmunoassay data correlated poorly with results by the four other methods

  3. Weltliche Einflüsse in der Notre-Dame-Musik? Überlegungen zu einer Klausel im Codex F

    Directory of Open Access Journals (Sweden)

    Büttner, Fred

    2002-12-01

    Full Text Available The unlimited validity of the rectilinear evolution that, according to Friedrich Ludwig, led from Notre Dame Clausula to Latin and thenceforth to French Motet was most recently questioned by Wolf Frobenius in his article "Zum genetischen Verhältnis zwischen Notre-Dame-Klauseln und ihren Motetten" (1987. Nevertheless, Frobenius does not thoroughly examine the pieces concerned, with hardly more far-reaching conclusions being attached to the priority claimed for many French Motets when comparing their concordances as Clausula and Latin Motet. With a setting of the choral melisma "ne" (Sm8 - already used by Ludwig in order to substantiate his estimation of the historical precedence of the Clausula - as an example, the point of view held by Frobenius is therefore once again subjected to critical scrutiny. Thus several traits can be established in the specifics of the Clausula that point to the field of secular music. Although this corroborates the considerations made by Frobenius, on the other hand such a result will open up new perspectives for further research into the School of Notre Dame.

    [de] Spätestens durch den Aufsatz „Zum genetischen Verhältnis zwischen Notre-Dame-Klauseln und ihren Motetten" von Wolf Frobenius (1987 wurde die uneingeschränkte Gültigkeit des geradlinigen Entwicklungsgangs, den Friedrich Ludwig von der Notre-Dame-Klausel über die lateinische bis zur franzosischen Motette zeichnete, in Frage gestellt. Jedoch bleibt Frobenius eine gründliche Untersuchung der betreffenden Stücke ebenso schuldig, wie auch an die behauptete Priorität vieler französischer Motetten gegenüber ihren Konkordanzen als Klausel und lateinische Motette kaum weiterreichende Schlußfolgerungen geknüpft werden. Anhand des „Ne"-Satzes Sm8, den bereits Ludwig exemplarisch hervorhob, um seine Auffassung von der Klausel als ältestem Stadium zu belegen, wird deshalb die Sichtweise von Frobenius noch einmal auf ihre Stichhaltigkeit

  4. Der grüne Weg zu Open Access: institutionelle und fachliche Repositorien / The Green Road to Open access: institutional and subject repositories

    Directory of Open Access Journals (Sweden)

    Bauer, Bruno

    2009-06-01

    Full Text Available The recent issue 1/2009 of GMS Medizin – Bibliothek – Information has a focus on „The green road to open Access: institutional and subject repositories“. Self-archiving and storing scholarly publications on a print server were also central topics in many presentations at the 9th International Bielefeld Conference in February 2009. The authors in this issue are Birgit Schmidt and Karin Ilg-Hartecke (Open Access in Deutschland – erweiterte Perspektiven für die Wissenschaft, Christoph Bruch and Anja Lengenfelder (Unterstützung des Grünen Weges zu Open Access an der Max-Planck-Gesellschaft, Ulrich Herb and Matthias Müller (Nuancen in Grün: Betrieb eines institutionellen und disziplinären Repositoriums – Erfahrungen und Entwicklungen an der Saarländischen Universitäts- und Landesbibliothek, Timo Borst and Jan B. Weiland (EconStor: ein fachliches Repositorium für die Wirtschaftswissenschaften, Antonella de Robbio and Michael Katzmayr (The management of an international open access repository: the case of E-LIS and Christian Gumpenberger (The EPrints story: Southampton as the cradle of institutional self-archiving.Furthermore this focus issue features an interview of a representative of a research funding organisation (Repositorien: Der grüne Weg zu Open Access Publishing aus der Perspektive einer Forschungsförderungsorganisation. 10 Fragen von Bruno Bauer an Falk Reckling, Mitarbeiter des FWF Der Wissenschaftsfonds and an interview of a publisher (Repositorien: Der grüne Weg zu Open Access Publishing aus der Perspektive der International Association of Scientific, Technical & Medical Publishers (STM: 10 Fragen von Bruno Bauer an Barbara Kalumenos, Director of Public Affairs bei STM.

  5. Erhöht ein Opfer-Täter-Zyklus das Risiko, Sexualstraftaten als pädosexuelle Straftaten zu begehen? : Ergebnisse einer ereignisanalytischen Pilotstudie

    OpenAIRE

    Urban, Dieter; Lindhorst, Heiko

    2004-01-01

    Die Pilotstudie untersucht mittels multivariater Ereignisanalysen den Übergang vom ehemaligen Opfer pädosexuellen bzw. körperlichen Missbrauchs zum pädosexuellen Täter im Lebensverlauf verurteilter Sexualstraftäter. Zur Erklärung dieses Übergangs werden aus den wichtigsten täterorientierten Theoriemodellen drei Hypothesen abgeleitet, die empirisch getestet werden sollen. Diese Hypothesen betreffen das Risiko, nach Opfererfahrungen in der Kindheit zum Täter pädosexuellen Missbrauchs zu werden....

  6. Erhöht ein Opfer-Täter-Zyklus das Risiko, Sexualstraftaten als pädosexuelle Straftaten zu begehen? : Ergebnisse einer ereignisanalytischen Pilotstudie

    OpenAIRE

    Urban, Dieter; Lindhorst, Heiko

    2004-01-01

    "Die Pilotstudie untersucht mittels multivariater Ereignisanalysen den Übergang vom ehemaligen Opfer pädosexuellen bzw. körperlichen Missbrauchs zum pädosexuellen Täter im Lebensverlauf verurteilter Sexualstraftäter. Zur Erklärung dieses Übergangs werden aus den wichtigsten täterorientierten Theoriemodellen drei Hypothesen abgeleitet, die empirisch getestet werden sollen. Diese Hypothesen betreffen das Risiko, nach Opfererfahrungen in der Kindheit zum Täter pädosexuellen Missbrauchs zu werden...

  7. Rezension zu: Thomas Ballhausen, Thomas Edlinger, Linda Henschel, Katherina Zakrawsky: The Porn Identity. Expeditionen in die Dunkelzone. Nürnberg: Verlag für Moderne Kunst 2009.

    Directory of Open Access Journals (Sweden)

    Florian Cramer

    2010-02-01

    Full Text Available Die Ausstellung „The Porn Identity“ und ihr begleitender Katalog- und Essayband kartografieren die Schnittmengen von Pornografie, zeitgenössischer Kunst und Kulturtheorie umfassend genug, um als Einführungen ins Themengebiet nützlich zu sein. Indem der Katalog Pornobilder und -lifestyle zugleich schwelgerisch zelebriert und genderpolitisch-korrekt verbrämt, ist er jedoch so schmierig wie kommerzielle Pornografie. Dies macht ihn zum Konzeptkunstwerk – und setzt Fragezeichen hinter den Diskurs der porn studies.

  8. Der Einfluss des Country-of-Origin Effekts im Vergleich zu anderen Faktoren auf die Kaufabsicht und Markenpräferenz in der Produktgruppe PKW

    OpenAIRE

    Gerhold, Christine

    2009-01-01

    Der Konsument sieht sich im zunehmenden Maße mit einer großen Produktvielfalt, einer allgemeinen Homogenisierung der Produkte und einer ständigen Informationsüberflutung konfrontiert. Zur leichteren Orientierung werden aus dem großen Bündel an Produktinformationen, einzelne "Schlüsselinformationen", verstärkt zur Kaufentscheidung herangezogen. In der vorliegenden Arbeit wird untersucht, welche Bedeutung die Schlüsselinformation "Country-of-Origin" im Vergleich zu anderen Faktoren, wie Marke o...

  9. Benefit analysis of e-business solutions for utilities; Nutzenbetrachtung von E-Business-Branchenloesungen. Der Online-Weg zu den Privatkunden

    Energy Technology Data Exchange (ETDEWEB)

    Koch, M.; Baier, D. [Lehrstuhl fuer Marketing und Innovationsmanagement und BTU Cottbus (Germany)

    2003-04-07

    E-Business-applications for internal and external business processes are an often dealt subject. In opposition to that the implementation of E-Business-Systems at the interface to the many private customers are taken only few notice. Even though the implementation requires additional investments utilities could save considerable costs. (orig.) [German] Die Unterstuetzung der internen und externen Geschaeftsprozesse durch E-Business-Systeme ist eine aufgrund ihrer Bedeutung oft behandelte Thematik. Weitgehend unbeachtet blieb bisher der Einsatz von E-Business-Systemen an der Schnittstelle zu den vielen Privatkunden. Trotz zusaetzlich notwendiger Investitionen lassen sich gerade an dieser Stelle hohe Kosten sparen. (orig.)

  10. Lipidomics by Supercritical Fluid Chromatography

    Directory of Open Access Journals (Sweden)

    Laurent Laboureur

    2015-06-01

    Full Text Available This review enlightens the role of supercritical fluid chromatography (SFC in the field of lipid analysis. SFC has been popular in the late 1980s and 1990s before almost disappearing due to the commercial success of liquid chromatography (LC. It is only 20 years later that a regain of interest appeared when new commercial instruments were introduced. As SFC is fully compatible with the injection of extracts in pure organic solvent, this technique is perfectly suitable for lipid analysis and can be coupled with either highly universal (UV or evaporative light scattering or highly specific (mass spectrometry detection methods. A short history of the use of supercritical fluids as mobile phase for the separation oflipids will be introduced first. Then, the advantages and drawbacks of SFC are discussed for each class of lipids (fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterols, prenols, polyketides defined by the LIPID MAPS consortium.

  11. Lipidomics by Supercritical Fluid Chromatography

    Science.gov (United States)

    Laboureur, Laurent; Ollero, Mario; Touboul, David

    2015-01-01

    This review enlightens the role of supercritical fluid chromatography (SFC) in the field of lipid analysis. SFC has been popular in the late 1980s and 1990s before almost disappearing due to the commercial success of liquid chromatography (LC). It is only 20 years later that a regain of interest appeared when new commercial instruments were introduced. As SFC is fully compatible with the injection of extracts in pure organic solvent, this technique is perfectly suitable for lipid analysis and can be coupled with either highly universal (UV or evaporative light scattering) or highly specific (mass spectrometry) detection methods. A short history of the use of supercritical fluids as mobile phase for the separation oflipids will be introduced first. Then, the advantages and drawbacks of SFC are discussed for each class of lipids (fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterols, prenols, polyketides) defined by the LIPID MAPS consortium. PMID:26090714

  12. Operating experience for a closed-circuit interconnected system for flue gas desulphurization at Klingenberg heating power station; Betriebserfahrungen zu einem Kreislaufverbundsystem fuer die Rauchgasentschwefelung im Heizkraftwerk Klingenberg

    Energy Technology Data Exchange (ETDEWEB)

    Hultsch, T [Berliner Kraft- und Licht (BEWAG)-AG, Heizkraftwerk Klingenberg, Berlin (Germany); Mueller, U [BDAG Balcke-Duerr AG, Ratingen (Germany)

    1995-05-01

    The cleaning of flue gases imposes extreme demands on the material concerned. By keeping below the acid dewpoint, this leads to condensation of acid products on the heating surfaces. The correct selection of the appropriate material is decisive for the availability of the overall plant. In the case of the application described in this paper, it has been possible to show that nickel-based alloys are outstandingly suitable for withstanding those kinds of stresses over many years. However, independently of this, it is valid to test material selection for each individual case of application. (orig.) [Deutsch] Die Reinigung von Rauchgasen stellt extreme Anforderungen an den jeweiligen Werkstoff. Durch die Unterschreitung des Saeuretaupunktes kommt es dabei zu einer Kondensation der Saeureprodukte auf den Heizflaechen. Die Auswahl des geeigneten Materials ist entscheidend fuer die Verfuegbarkeit der Gesamtanlage. In dem hier dargestellten Anwendungsfall konnte gezeigt werden, dass Nickelbasis-Legierungen hervorragend geeignet sind, einer derartigen Beanspruchung ueber Jahre hinaus standzuhalten. Unabhaengig hiervon gilt es jedoch, fuer die Materialauswahl jeden Anwendungsfall einzeln zu pruefen. (orig.)

  13. „Weder dem Vergangenen anheimfallen noch dem Zukünftigen. Es kommt darauf an, ganz gegenwärtig zu sein“

    Directory of Open Access Journals (Sweden)

    Christian Filk

    2012-03-01

    Full Text Available Aufwachsen und Leben in der Wissensgesellschaft wird schon länger durch die Diskussion um ‚Informationsarme‘ und ‚Informationsreiche‘ begleitet. Jüngere wissenschaftliche Studien belegen das mangelnde oder gar nicht vorhandene Wissen zu wichtigen politischen, geschichtlichen und ethischen Themen wie Nationalsozialismus und Shoah. Christian Filk untersucht wesentliche Grundsätze moderner Geschichtsschreibung und den angemessenen Einsatz von Wissensmedien. Schliesslich erörtert er, wie elektronische, digitale und soziale Medien dazu beitragen können, mehr über den Genozid von etwa sechs Millionen europäischen Juden während des Zweitens Weltkriegs zu erfahren.For some time, growing up and living in a knowledge-based society is accompanied by the discussion on ‚information poor‘ and ‚information rich‘. Recent Scientific studies show the lack or non-existing knowledge on important political, historical and ethical issues such as Nazism and the Holocaust. Christian Filk examines essential principles of modern history and appropriate use of knowledge media. Finally he discusses, how electronic, digital and social media could help to learn more about the genocide of six million European Jews during World War II.

  14. Review of preliminary results of the project `Co-cracking of plastics and petroleum residues`; Uebersicht zu den ersten Ergebnissen des Projektes Cocracking von Kunststoffen und Erdoelrueckstand

    Energy Technology Data Exchange (ETDEWEB)

    Uhmann, R; Koepsel, R F; Kuchling, T; Simanjenkov, V [Technische Univ. Bergakademie Freiberg (Germany). Inst. fuer Energieverfahrenstechnik und Chemieingenieurwesen

    1998-09-01

    Coprocessing of petroleum residues and plastics is a promising technology, although some problems concerning waste plastics must be clarified prior to its implementation. In general, it can be stated that with a careful choice of operating parameter combinations, oil yields will be higher than for thermal treatment of petroleum residues alone (e.g. a 35% yield increase is achieved by adding 25% of plastics, which is a disproportionately high increase). Waste plastics thus become a valuable material for processing. (orig.) [Deutsch] Das untersuchte Coprocessing von VR und Kunststoffen ist ein erfolgversprechender Weg der Kunststoffverwertung und des tiefen Crackens von Erdoelrueckstand. Die Klaerung der mit Altkunststoffen verbundenen Fragen bedarf weiterer Untersuchungen. Allgemein kann festgestellt werden, dass bei entsprechenden Parameterkombinationen bessere Oelausbeuten erzielt werden als bei der thermischen Behandlung von reinem VR. Ein Beispiel dafuer ist in der Abbildung 10 dargestellt. Durch eine 25%-ige Erhoehung der Einsatzstoffmasse durch Kunststoffzugabe wird eine im Vergleich zu reinem VR um ca. 36% hoehere Oelausbeute erzielt, was einer ueberproportionalen Erhoehung entspricht. Altkunststoff wird bei diesem Prozess unter Nutzung des Wasserstoff-potentials von Polymeren zu einem wertvollen Einsatzstoff. (orig.)

  15. Idle load losses of an injection-lubricated spur gear drive; Untersuchungen zu den Leerlaufverlusten eines einspritzgeschmierten Stirnradgetriebes

    Energy Technology Data Exchange (ETDEWEB)

    Dick, A.K.

    2000-07-01

    Arbeitsmaschine in Reihe geschaltet sind, wirkt sich dies direkt auf den Wirkungsgrad der Anlage aus. Mit dem im Rahmen dieser Arbeit gebauten Pruefstand wurde die Druckabhaengigkeit der lastunabhaengigen Verzahnungsverluste eines Stirnradpaares bis zu Umfangsgeschwindigkeiten von v{sub t}=200 m/s fuer Druecke von p{sub G}=0.13 bar / 1.4 bar ermittelt. Die gemessenen Ventilations- und Quetschverluste sind linear vom Druck abhaengig. Die aus bekannten Potenzansaetze zur Berechnung der lastunabhaengigen Verzahnungsverluste wurden bezueglich der Impulsverluste korrigiert. Ausserdem wurde der Faktor F{sub Druck} zur Beschreibung der Druckabhaengigkeit von Ventilations- und Quetschverlusten neu eingefuehrt. Die Stroemungsvorgaenge im Getriebegehaeuse beeinflussen die lastunabhaengigen Verluste. Durch das Teilvakuum werden die Stroemungsvorgaenge im Getriebe veraendert. Aehnliche Effekte koennen u.U. durch eine enge Kapselung der Rotoren erzielt werden. Die Versuchsergebnisse zeigen, dass das Potential fuer eine weitere Verlustreduzierung bei niedrigen Druecken gering ist. Da jedoch auch in Zukunft Turbogetriebe bei Atmosphaerendruck betrieben werden, sollte der Einfluss von eng anliegenden Getriebegehaeusen untersucht werden. (orig./AKF)

  16. Hugo Chavez : le renouveau du populisme vénézuélien ?

    Directory of Open Access Journals (Sweden)

    Olivier Folz

    2005-09-01

    Full Text Available Pour aborder la problématique du populisme en tant que phénomène politique, nous avons choisi de nous placer dans une perspective latino-américaine en nous intéressant au cas particulier du Venezuela et de son Président Hugo Chavez. La crise généralisée qui, à partir des années quatre-vingts, s’est abattue sur le pays a mis un terme à quarante années de bipartisme et a considérablement affaibli le modèle de démocratie représentative mis en place par les partis politiques Action Démocratique et Copei. Profitant de ce vide, de nouveaux acteurs politiques, indépendants des partis traditionnels, émergèrent dans le panorama politique vénézuélien et parmi eux Hugo Chavez. Ex-putschiste et leader incontesté des secteurs défavorisés, il construisit sa victoire aux élections présidentielles de 1998 en s’appuyant sur un discours virulent envers les partis et l’ancienne classe politique. Grâce à cette technique, Chavez sut capter l’attention des laissés-pour-compte et des déçus du système en s’imposant comme le candidat de la rupture, du changement en faveur d’une démocratie participative et du retour à l’âge d’or, inspiré de Bolivar, jugé plus à même de conduire le Venezuela vers un avenir meilleur.Afin d’appréhender les principales caractéristiques de ce leader charismatique, dans un premier temps, après une brève étude du populisme vénézuélien des années cinquante, nous nous concentrerons sur le personnage, puis, à travers ses déclarations et ses écrits, nous examinerons le diagnostic qu’il porte sur la crise que traverse le Venezuela, la nature de son programme électoral et, enfin, son rapport au peuple. Grâce à ces différents axes de recherches et à leur analyse, nous verrons si Hugo Chavez est ou non un leader populiste.Con el propósito de abordar la problemática del populismo como fenómeno político, hemos decidido ubicarnos en una perspectiva latinoamericana interes

  17. Immune chromatography: a quantitative radioimmunological assay

    International Nuclear Information System (INIS)

    Davis, J.W.; Demetriades, M.; Bowen, J.M.

    1984-01-01

    Immune chromatography, a radioimmunological binding assay, employs paper chromatography to separate immune complexes from free antigen and antibodies. During chromatography free antigen and antibodies become distributed throughout the paper, while immune complexes remain near the bottoms of the strips. The chromatographic differences can be made quantitative by using either iodinated antigens or antibodies. Under these conditions nanogram quantities of antigen can be detected or antibodies in sera diluted several 1000-fold. The immune chromatography assay can also be performed as an indirect assay, since the paper strips are cut from nitrocellulose paper. In this case the immune components are absorbed by the paper during chromatography. Antigen is then detected with an iodinated second antibody. The indirect immune chromatography assay is particularly useful for identifying different sera that react with the same antigen. Reaction with the first serum before chromatography reduces the amount of antigen available to the second serum following chromatography. In addition to characterizing the immune chromatography procedure, we discuss the possible applications of chromatography assays for the quantitation of other types of molecular binding interactions. (Auth.)

  18. Ion mobility: its role in plasma chromatography

    International Nuclear Information System (INIS)

    Mason, E.A.

    1984-01-01

    This paper is a review of the basic physical theory underlying plasma chromatography. Essentially, plasma chromatography simply measures ion mobility. The new feature of plasma chromatography, as compared to aqueous electrophoresis, is the existence of a highly-developed and accurate body of theory that connects gaseous ion mobility and diffusion to the ion molecule interactions in the drift tube. Attention is restricted to phenomena occurring in the drift tube portion of the apparatus

  19. Characterisation of uremic "Middle molecular"fractions by gas chromatography mass spectrometry, isotachophoresis, and liquid chromatography

    NARCIS (Netherlands)

    Schoots, A.C.; Mikkers, F.E.P.; Claessens, H.A.; Smet, de R.; Landschoot, van N.; Ringoir, S.M.G.

    1982-01-01

    Uremic ultrafiltrates (and normal serum, for comparison) were fractionated by means of gel filtration. The collected fractions were further investigated by combined analytical techniques: "high- performance" liquid chromatography, gas chromatography, mass spectrometry, and isotachophoresis.

  20. RNase-assisted RNA chromatography

    Science.gov (United States)

    Michlewski, Gracjan; Cáceres, Javier F.

    2010-01-01

    RNA chromatography combined with mass spectrometry represents a widely used experimental approach to identify RNA-binding proteins that recognize specific RNA targets. An important drawback of most of these protocols is the high background due to direct or indirect nonspecific binding of cellular proteins to the beads. In many cases this can hamper the detection of individual proteins due to their low levels and/or comigration with contaminating proteins. Increasing the salt concentration during washing steps can reduce background, but at the cost of using less physiological salt concentrations and the likely loss of important RNA-binding proteins that are less stringently bound to a given RNA, as well as the disassembly of protein or ribonucleoprotein complexes. Here, we describe an improved RNA chromatography method that relies on the use of a cocktail of RNases in the elution step. This results in the release of proteins specifically associated with the RNA ligand and almost complete elimination of background noise, allowing a more sensitive and thorough detection of RNA-binding proteins recognizing a specific RNA transcript. PMID:20571124

  1. Application of gas chromatography in hydrogen isotope separation

    International Nuclear Information System (INIS)

    Ye Xiaoqiu; Sang Ge; Peng Lixia; Xue Yan; Cao Wei

    2008-01-01

    The principle of gas chromatographic separation of hydrogen isotopes was briefly introduced. The main technology and their development of separating hydrogen isotopes, including elution chromatography, hydrogen-displacement chromatography, self-displacement chromatography and frontal chromatography were discussed in detail. The prospect of hydrogen isotope separation by gas chromatography was presented. (authors)

  2. Extraprostatische Expansion des Prostatakarzinoms und seine Beziehung zu den Resektionsrändern bei der radikalen Prostatektomie aus Sicht des Pathologen

    Directory of Open Access Journals (Sweden)

    Noll P

    2008-01-01

    Full Text Available Das Prostatakarzinom stellt heute in der westlichen Welt das am häufigsten diagnostizierte Karzinom beim Mann dar. Entsprechend dieser Häufigkeit werden zunehmend radikale Prostatektomien durchgeführt. Die histologische Aufarbeitung des Operationspräparates liefert dem Urologen die wichtigsten Prognosefaktoren des Prostatakarzinoms. Hierbei besteht ein enger Zusammenhang zwischen der extraprostatischen Ausdehnung des Tumors und seiner Beziehung zu den Resektionsrändern. Anhand einer eigenen Untersuchung an 200 radikal operierten Prostatakarzinomen konnte gezeigt werden, dass hierbei die Lokalisation, der Malignitätsgrad und die Größe des Tumors entscheidende Bedeutung haben. Die Ermittlung dieser Parameter ist abhängig von einer standardisierten Untersuchungstechnik auf der Grundlage einer vollständigen histologischen Aufarbeitung der Prostata.

  3. No Escape from the Burbs. Rezension zu Roger Keil (Hg. (2013: Suburban Constellations: Governance, Land and Infrastructure in the 21st Century. Berlin: JOVIS.

    Directory of Open Access Journals (Sweden)

    Markus Kip

    2016-11-01

    Full Text Available Den Begriff des Urbanen in Auseinandersetzung mit dem Suburbanen zu bestimmen, ist horizonterweiternd. Nicht, dass er nach der Lektüre von Suburban Constellations an Schärfe gewonnen hätte, wohl aber hat sich meine Aufmerksamkeit für scheinbar banale Phänomene außerhalb der Stadt geschärft. Neu ist die enorme Bandbreite suburbaner Fallstudien aus dem ‚Globalen Norden‘ und dem ‚Globalen Süden‘, die hier versammelt werden.Der Band ist ein wichtiger Output des Forschungsverbunds „Global Suburbanism. Governance, Land and Infrastructure in the 21st Century“, dem über fünfzig Wissenschaftler_innen und Praktiker_innen angehörenAm Ende hat mich die Hauptthese des Buches überzeugt, der zufolge suburbane Räume keine Randphänomene, sondern Schlüsselaspekte der Urbanisierung im 21. Jahrhundert darstellen.

  4. Physical interactions in MRI. Some rules of thumb for their reduction; Physikalische Wechselwirkungen in der MRT. Einige Daumenregeln zu ihrer Reduktion

    Energy Technology Data Exchange (ETDEWEB)

    Muehlenweg, M. [Krankenhaus Martha-Maria Halle-Doelau, Institut fuer Radiologie, Halle (Saale) (Germany); Schaefers, G. [MR:comp GmbH, Gelsenkirchen (Germany); Trattnig, S. [Medizinische Universitaet Wien, Exzellenzzentrum Hochfeld-Magnetresonanz, Universitaetsklinik fuer Radiodiagnostik, Wien (Austria)

    2015-08-15

    Magnetic resonance imaging (MRI) is one of the most powerful and at the same time gentlest clinical imaging techniques at the present time; however, the enormous physical complexity as well as simple inattentiveness (projectile effect) implicate a significant risk potential and place high demands on the MR operator to ensure a safe workflow. A sound knowledge of the potential MR interactions is the foundation for a safe and profitable operation for all parties. The first part of this article deals with the three most important sources of physical interaction, i.e. static magnetic field, gradient and high-frequency (HF) fields. The paper discusses the differences between each type of field with respect to the impact on human beings, the interactions with magnetic and electrically conducting objects/implants and the relevant safety standards. Each section is followed by simple rules of thumb to minimize potentially unwanted physical MRI interactions. (orig.) [German] Die Magnetresonanztomographie ist eines der leistungsfaehigsten und zugleich schonendsten klinischen bildgebenden Verfahren der heutigen Zeit. Allerdings bergen ihre enorme physikalische Komplexitaet, aber auch einfache Unaufmerksamkeiten (''Projektileffekt'') ein signifikantes Risikopotenzial in sich und stellen hohe Anforderungen an die MR-Bediener, um einen sicheren Betrieb zu gewaehrleisten. Ein solides Wissen um die potenziellen MR-Wechselwirkungen ist die Grundlage fuer einen sicheren und fuer alle Seiten gewinnbringenden Betrieb. Der erste Teil der Arbeit behandelt die 3 zentralen Quellen fuer physikalische Wechselwirkungen in der Magnetresonanztomographie (statisches Magnetfeld, geschaltete Gradienten- und HF-Felder). Es werden fuer jede Feldart die Auswirkungen auf den Menschen, Wechselwirkungen mit magnetischen und elektrisch leitenden Objekten/Implantaten und relevante Sicherheitsstandards besprochen. Daran angeschlossen ist jeweils ein Abschnitt mit einfachen &apos

  5. Thin-layer and paper chromatography

    International Nuclear Information System (INIS)

    Sherma, J.

    1986-01-01

    This selective review covers the literature of thin-layer chromatography (TLC) and paper chromatography (PC) cited in Chemical Abstracts from December 5, 1983, through November 25, 1985, and Analytical Abstracts from November 1983 to November 1985. Also researched directly were the following important journals publishing papers on TLC and PC: the Journal of Chromatography (including its bibliography issues), Journal of High Resolution Chromatography and Chromatography Communications, Journal of Chromatographic Science, Chromatographia, Analytical Chemistry, JAOAC, and the special TLC issues of the Journal of Liquid Chromatography. Many of the inherent advantages of TLC that are obvious to workers familiar with high performance, quantitative theory and practice still are not appreciated adequately by the majority of people using chromatography. These include unrestricted access to the separation process; introducing magnetic, thermal, electrical, and other physical forces to improve resolution; high sample throughput; truly multidimensional separations; and the use of controlled multiple gradients. Many advantages of TLC relative to column chromatography were discussed in the Introductions to our 1982 and 1984 reviews of TLC in this Journal. No complete commercial robotics system specifically for TLC has been developed, but all necessary modules are available for such a system. The combination of robotics, with the continued development of theory, practice, and instrumentation will lead eventually to TLC systems that are unrivaled for speed, versatility, accuracy, precision, and sensitivity. 573 references

  6. Chromium Speciation Analysis by Ion Chromatography Coupled ...

    African Journals Online (AJOL)

    Two methods coupling ion chromatography with inductively coupled plasma - optical emission spectroscopy (ICP-OES) were developed for the simultaneous separation and determination of Cr(III) and Cr(VI) species. In the first method, anion chromatography with sodium bicarbonate/carbonate solution as the eluent was ...

  7. Ein Versuch, Naturverhältnisse aus feministischer Sicht zu interpretieren Another attempt to explain the natural environment from a feminist perspective

    Directory of Open Access Journals (Sweden)

    Helga Purgand

    2002-07-01

    Full Text Available Mit dem vorliegenden Sammelband Geschlechterverhältnisse – Naturverhältnisse wird nach dem innovativen Beitrag der feministischen Debatte für umweltsoziologisches Denken gefragt und nach Überschneidungen, Grenzen und Zwischenräumen zwischen „Geschlecht“ und „Natur“ gesucht. In ihm wird der lobenswerte Versuch unternommen, einen Zusammenhang zwischen Geschlechter- und Naturverhältnissen herzustellen „die in der Umweltsoziologie bislang stark vernachlässigte feministische Diskussion und Geschlechterforschung einzubeziehen und als genderbezogene Perspektive zu etablieren“. (S. 9 Das Buch enthält Beiträge von 15 Autorinnen, die unterschiedliche Natur- und Geschlechtsverhältnisse facettenreich, überwiegend kreativ und theoretisch anspruchsvoll betrachten. Das Buch enthält Zugänge aus der allgemeinen Soziologie, den Sprach- und Kulturwissenschaften, aus politik- und naturwissenschaftlichen Zusammenhängen. Den ausdrücklich formulierten Anspruch, von multi- zu einer inter-disziplinären Durchdringung zu gelangen, erfüllt es nur in Ansätzen (besonders mit der Einleitung und den theoretischen Problemexplikationen. Das zustande gekommene „Nach-, Zusammen- und Querdenken“ über Begriffe, Theorien und neuere Forschungsansätze (von Foucault über Butler bis zu Donna Haraway belegt die Notwendigkeit, die naturwissenschaftlichen Annahmen „immanent zu dekonstruieren“. Sehr aufschlussreich sind in diesem Zusammenhang die Beiträge von Irmgard Schultz, Elvira Scheich, aber auch von Mary Mellor und besonders von Paula-Irene Villa, die interessante und kreative Gedanken zur Klärung des Verhältnisses von Natur, Kultur und Geschlecht anbieten. Deutlich wurde, dass weiterhin ein kontroverses Ringen um angemessene konzeptionelle Fassungen von Geschlechts- und Naturverhältnissen und die Integration von Gender als zentraler analytischer Querschnitts-Kategorie nötig sind. Insgesamt ist dem Buch ist eine schnelle Verbreitung und

  8. Dielectrokinetic chromatography and devices thereof

    Science.gov (United States)

    Chirica, Gabriela S; Fiechtner, Gregory J; Singh, Anup K

    2014-04-22

    Disclosed herein are methods and devices for dielectrokinetic chromatography. As disclosed, the devices comprise microchannels having at least one perturber which produces a non-uniformity in a field spanning the width of the microchannel. The interaction of the field non-uniformity with a perturber produces a secondary flow which competes with a primary flow. By decreasing the size of the perturber the secondary flow becomes significant for particles/analytes in the nanometer-size range. Depending on the nature of a particle/analyte present in the fluid and its interaction with the primary flow and the secondary flow, the analyte may be retained or redirected. The composition of the primary flow can be varied to affect the magnitude of primary and/or secondary flows on the particles/analytes and thereby separate and concentrate it from other particles/analytes.

  9. Detection in superheated water chromatography

    International Nuclear Information System (INIS)

    Chienthavorn, O.

    1999-11-01

    Superheated water has been used successfully as an eluent in liquid chromatography and has been coupled to various modes of detection, ultraviolet (UV), fluorescence, and nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS). A number of compounds were examined on poly(styrene-divinylbenzene) (PS-DVB), polybutadiene (PBD), and octadecylsilyl bonded silica (ODS) column with isothermal and temperature programmes. The PS-DVB column was mostly used throughout the project as it was the most stable. Not only pure water could serve as superheated water mobile phase; inorganic buffered water and ion-pairing reagent with a concentration of 1-3 mM of the buffer and reagent were also exploited. It was shown that the pH could be controlled during the separation without salt precipitation and the separations followed a conventional reversed-phase HPLC method. Results from fluorescence detection showed good separation of a series of vitamins, such as pyridoxine, riboflavin, thiamine, and some analgesics. The relationship of riboflavin using the detection was linear and the detection limit was seven times higher than that of a conventional method. Simultaneous separation and identification using superheated water chromatography-NMR was demonstrated. With using a stop flow method, NMR spectra of model drugs, namely barbiturates, paracetamol, caffeine and phenacetin were obtained and the results agreed with reference spectra, confirming a perfect separation. A demonstration to obtain COSY spectrum of salicylamide was also performed. The method was expanded to the coupling of superheated water LC to NMR-MS. Results from the hyphenated detection method showed that deuteration and degradation happened in the superheated water conditions. The methyl group hydrogens of pyrimidine ring of sulfonamide and thiamine were exchanged with deuterium. Thiamine was decomposed to 4-methyl-5-thiazoleethanol and both were deuterated under the conditions. (author)

  10. Dohmen, Dieter, Erbes, Annegret, Fuchs, Kathrin & Günzel, Juliane (2008). Was wissen wir über Nachhilfe? Sachstand und Auswertung der Forschungsliteratur zu Angebot, Nachfrage und Wirkungen. Bielefeld: Bertelsmann, 150 S. [Rezension

    OpenAIRE

    Mayr, Thomas

    2014-01-01

    Rezension von Dohmen, Dieter, Erbes, Annegret, Fuchs, Kathrin & Günzel, Juliane (2008). Was wissen wir über Nachhilfe? Sachstand und Auswertung der Forschungsliteratur zu Angebot, Nachfrage und Wirkungen. Bielefeld: Bertelsmann, 150 S. 29,90 EUR, ISBN 978-3763936656

  11. Emotions as a site-related factor. The attitudes of the population towards energy policy and environmental protection; Emotionen als Standortfaktor. Die Einstellungen der Bevoelkerung zu Energiepolitik und Umweltschutz

    Energy Technology Data Exchange (ETDEWEB)

    Koecher, R. [Inst. fuer Demoskopie, Allensbach (Germany)

    1997-06-01

    For years, debate has been raging on the future competitiveness of Germany. The discussions focus on wages and incidental wage costs, working hours, the education and training system, technological changes, flexibilization and the fight against red tape as well as social consensus. But one site-related factor hardly crops up in public discussion and in economic-science textbook and expertises, although it quite essentially influences competitiveness, now even more than formerly: the mentality of the population. It includes priorities held by society, target concepts such as the openness of the population towards innovation and its attitude towards technology and technical progress as well as the way in which discussions are conducted in society and decisions taken, especially also people`s inclination to rationality or irrationality, and proneness to emotions or ideological thinking. (orig.) [Deutsch] Seit Jahren wird ueber die Zukunft des Standortes Deutschland diskutiert und gestritten. Im Mittelpunkt der Diskussion stehen Loehne und Lohnnebenkosten, Arbeitszeiten, das Bildungs- und Ausbildungssystem, technologische Veraenderungen, Flexibilisierung und Entbuerokratisierung und der soziale Frieden. Ein Standortfaktor kommt dagegen in der oeffentlichen Diskussion wie auch in den wirtschaftswissenschaftlichen Lehrbuechern und Expertisen kaum vor, obwohl er die Standortqualitaet ganz wesentlich beeinflusst, heute noch mehr als frueher: Die Mentalitaet der Bevoelkerung. Dazu gehoeren die gesellschaftlichen Prioritaeten und Zielvorstellungen wie die Innovationsoffenheit der Bevoelkerung, ihre Einstellung zu Technik und technischem Fortschritt wie die Art, gesellschaftliche Diskussionen zu fuehren und Entscheidungen zu treffen, besonders auch die Neigung zu Rationalitaet und Irrationalitaet, die Anfaelligkeit fuer Emotionen und Ideologisierung. (orig.)

  12. [Stadt und Orden. Das Verhältnis des deutschen Ordens zu den Städten in Livland, Preußen und im Deutschen Reich] / Bernhart Jähnig

    Index Scriptorium Estoniae

    Jähnig, Bernhart, 1941-

    1995-01-01

    Arvustus: Stadt und Orden. Das Verhältnis des deutschen Ordens zu den Städten in Livland, Preußen und im Deutschen Reich, hg. v. Udo Arnold. [Quellen und Studien zur Geschichte des Deutschen Ordens. 44; Veröffentlichungen der Internationalen Historischen Kommission zur Erforschung des Deutschen Ordens. 4) Marburg: N. G. Elwert Verlag 1993. VIII, 311 S.

  13. [Staat und Orden. Das Verhältnis des Deutschen Ordens zu den Städten in Livland, Preußen und im Deutschen Reich] / Bernhart Jähnig

    Index Scriptorium Estoniae

    Jähnig, Bernhart, 1941-

    1995-01-01

    Arvustus: Staat und Orden. Das Verhältnis des Deutschen Ordens zu den Städten in Livland, Preußen und im Deutschen Reich, hg. v. Udo Arnold. (Quellln und Studien zur Geschichte des Deutschen Ordens. 4.) Marburg: N. G. Elwert Verlag 1993

  14. Überlegungen zu einer entrepreneur-orientierten Ausbildung an gewerblichen Fachschulen: eine Bestandsaufnahme mit Vorschlägen für eine Ergänzung des Curriculums

    OpenAIRE

    Hartmann, Falk

    2010-01-01

    In der Arbeit wird untersucht ob eine Ausbildung zum Entrepreneur, also zur Selbständigkeit möglich ist und es wird ein geeigneter curricularer Ansatz gesucht eine Entrepreneurship Education in die Ausbildung zum staatlich geprüften Techniker Maschinentechnik zu integriereren.

  15. [Dennis Hormuth. Livonia est omnis divisa in partes tres : Studien zu mental mapping der livländischen Chronistik in der Frühen Neuzeit (1558-1710) ] / Stefan Donecker

    Index Scriptorium Estoniae

    Donecker, Stefan, 1977-

    2014-01-01

    Arvustus: Hormuth, Dennis. Livonia est omnis divisa in partes tre : Studien zu mental mapping der livländischen Chronistik in der Frühen Neuzeit (1558-1710). (Quellen und Studien zur Geschichte des östlichen Europa, 79). Stuttgard, 2012

  16. Analiza wielkości i zmienności zużycia wody w kampusie Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie

    Directory of Open Access Journals (Sweden)

    Piotr Wichowski

    2015-10-01

    Full Text Available W artykule przedstawiono badania wielkości i zmienności zużycia wody w kampusie SGGW w roku akademickim 2012/13. Przeciętne jednostkowe zużycie wody w okresie, kiedy realizowane są zajęcia dydaktyczne, wyniosło 29,3 dm3·d–1 dla studenta studiów stacjonarnych i 23,2 dm3·d–1 dla studenta studiów niestacjonarnych. W okresie wakacyjnym wskaźniki te są niższe i wynoszą odpowiednio 15,2 oraz 11,4 dm3·d–1. Wskaźniki te wyznaczono bez uwzględnienia zużycia wody w akademikach. Współczynnik nierównomierności dobowej dla całego kampusu wyniósł 1,54 i był zbliżony do współczynnika nierównomierności godzinowej, wynoszącego 1,52. Najwyższe miesięczne zużycie wody wystąpiło w czerwcu i było o 85% większe od najniższego, które wystąpiło w sierpniu. W cyklu tygodniowym najwyższe zużycie wody obserwowano we wtorki, a najniższe w niedziele. Przeciętne rozbiory wtorkowe są wyższe od rozbiorów niedzielnych o 31,5%. Szczyty rozbiorów w ciągu doby przypadały w godzinach 10–15 oraz 23–01.

  17. Analytical chromatography. Methods, instrumentation and applications

    International Nuclear Information System (INIS)

    Yashin, Ya I; Yashin, A Ya

    2006-01-01

    The state-of-the-art and the prospects in the development of main methods of analytical chromatography, viz., gas, high performance liquid and ion chromatographic techniques, are characterised. Achievements of the past 10-15 years in the theory and general methodology of chromatography and also in the development of new sorbents, columns and chromatographic instruments are outlined. The use of chromatography in the environmental control, biology, medicine, pharmaceutics, and also for monitoring the quality of foodstuffs and products of chemical, petrochemical and gas industries, etc. is considered.

  18. Radiochemical purity determination by paper chromatography 2

    International Nuclear Information System (INIS)

    1975-01-01

    The standard relates to the determination of radiochemical impurities in labelled compounds using paper chromatography. The basic terms are given as is the description of procedure and evaluation of chromatograms. (E.S.)

  19. Ion Chromatography Applications in Wastewater Analysis

    Directory of Open Access Journals (Sweden)

    Rajmund Michalski

    2018-02-01

    Full Text Available Wastewater analysis is an important area in analytical and environmental chemistry. It can be performed with both the classic wet methods and instrumental techniques. The development of new methods, and modification of the existing ones, constitute a major task for researchers. Ion chromatography plays a predominant role in ion determinations with the instrumental methods. It offers several advantages over the conventional methods, such as simultaneous determinations of alkali and alkaline earth cations and ammonia. Ammonium ions cannot be determined by spectroscopic methods. Ion chromatography has been accepted world-wide as a reference method for analyzing anions and cations in water and wastewater due to the fact that it enables the replacement of several individual wet chemistry methods for common ions with one instrumental technique. The following article describes the principles of ion chromatography, such as stationary phases, eluents, detectors, and sample preparation methods. Moreover, the applications of ion chromatography in wastewater analyses and international standards are presented.

  20. Gradient High Performance Liquid Chromatography Method ...

    African Journals Online (AJOL)

    Purpose: To develop a gradient high performance liquid chromatography (HPLC) method for the simultaneous determination of phenylephrine (PHE) and ibuprofen (IBU) in solid ..... nimesulide, phenylephrine. Hydrochloride, chlorpheniramine maleate and caffeine anhydrous in pharmaceutical dosage form. Acta Pol.

  1. UHPLC: The Greening Face of Liquid Chromatography

    OpenAIRE

    Cielecka-Piontek, Judyta; Zalewski, Przemys?aw; Jeli?ska, Anna; Garbacki, Piotr

    2013-01-01

    Pharmaceutical analysis based on chromatographic separation is an important part of studies aimed at developing routine quality analysis of drugs. High-performance liquid chromatography (HPLC) is one of the main analytical techniques recommended for drug analysis. Although it meets many criteria vital for analysis, it is time-consuming and uses a relatively high amount of organic solvents compared to other analytical techniques. Recently, Ultra-high-performance liquid chromatography (UHPLC) h...

  2. Selection of imprinted nanoparticles by affinity chromatography.

    Science.gov (United States)

    Guerreiro, António R; Chianella, Iva; Piletska, Elena; Whitcombe, Michael J; Piletsky, Sergey A

    2009-04-15

    Soluble molecularly imprinted nanoparticles were synthesised via iniferter initiated polymerisation and separated by size via gel permeation chromatography. Subsequent fractionation of these particles by affinity chromatography allowed the separation of high affinity fractions from the mixture of nanoparticles. Fractions selected this way possess affinity similar to that of natural antibodies (K(d) 6.6x10(-8)) M and were also able to discriminate between related functional analogues of the template.

  3. Large scale chromatographic separations using continuous displacement chromatography (CDC)

    International Nuclear Information System (INIS)

    Taniguchi, V.T.; Doty, A.W.; Byers, C.H.

    1988-01-01

    A process for large scale chromatographic separations using a continuous chromatography technique is described. The process combines the advantages of large scale batch fixed column displacement chromatography with conventional analytical or elution continuous annular chromatography (CAC) to enable large scale displacement chromatography to be performed on a continuous basis (CDC). Such large scale, continuous displacement chromatography separations have not been reported in the literature. The process is demonstrated with the ion exchange separation of a binary lanthanide (Nd/Pr) mixture. The process is, however, applicable to any displacement chromatography separation that can be performed using conventional batch, fixed column chromatography

  4. Überzeugen, Stupsen, Zwingen – Die Konzeption von Nudge und Libertärem Paternalismus und ihr Verhältnis zu anderen Formen der Verhaltenssteuerung

    Directory of Open Access Journals (Sweden)

    Düber Dominik

    2016-07-01

    Full Text Available Trotz der überwältigenden öffentlichen wie wissenschaftlichen Resonanz, auf die der Nudge-Ansatz von Thaler und Sunstein gestoßen ist, bleiben die genauen Konturen dieses Theorie und Policyvorschlages unscharf. So ist an vielen Stellen unklar, worin die spezifische Differenz gegenüber klassischen Formen der Verhaltenssteuerung liegen soll. Für eine Bewertung des Ansatzes in seinen Vorzügen und Nachteilen gegenüber klassischen Steuerungsformen ist eine solche klärende Einordnung jedoch notwendige Vorbedingung. Der Aufsatz arbeitet daher die bestehenden Definitions- und Abgrenzungsprobleme von „Nudge“ und „Libertärem Paternalismus“ heraus und plädiert sodann für einen Kernbegriff von „Nudge“, der dessen Extension einschränkt, um ihn als originären und eigenständigen Vorschlag einzugrenzen. Ein so präzisiertes Verständnis von Nudge wird dann hinsichtlich seiner Eigenschaften sowie der Gemeinsamkeiten und Unterschiede zu klassischen Formen der Verhaltenssteuerung ins Verhältnis gesetzt.

  5. Wäre es Dir lieber, ich würde zu Recht verurteilt? - Sokrates Bund und die Frage der Kontrolle

    Directory of Open Access Journals (Sweden)

    Katharina Kaiser-Müller

    2014-09-01

    Full Text Available Die Ablösung von zweifelhafter menschlicher Kontrolle durch eine zuverlässige Software bietet eindrucksvolle Möglichkeiten. Mit der flächendeckenden Einführung von Sokrates Bund zum Schuljahr 2014/2015 wird endlich die Identifzierung von Pisadurchschnittsverderbern möglich. Die AutorInnen diskutieren die Lage ... Die Situation war dramatisch: Ungerecht angeklagt wegen Verführung der Jugend und Gottlosigkeit, sieht Sokrates dem Tod durch den Schierlingsbecher entgegen und antwortet auf die ob des Fehlurteils durchaus verständliche Klage von Xanthippe mit der titelgebenden Frage. Anschließend verweigert er die Flucht und zieht es vor, sich dem formal korrekt gefällten falschen Urteil zu beugen. Eine legendäre Entscheidung, in der Sokrates die Gesetzestreue mit der Begründung, man müsse schlechte Gesetze ändern, dürfe sie aber nicht übertreten, über das eigene Wohl stellt.

  6. Gestaltung einer netzbasierten Lernumgebung für einen Fernstudiengang zu „Medien und Informationstechnologien in Erziehung, Unterricht und Bildung“

    Directory of Open Access Journals (Sweden)

    Gerhard Tulodziecki

    2017-07-01

    Full Text Available In diesem Beitrag stelle ich dar, wie wir in einem kooperativen Verbund eine netzbasierte Lernumgebung für einen Fernstudiengang zu „Medien und Informationstechnologien in Erziehung, Unterricht und Bildung“ gestalten. Das Projekt wird von zwei Arbeitsgruppen an der Universität Paderborn (Allgemeine Didaktik und Medienpädagogik, Didaktik der Informatik und einer Arbeitsgruppe an der Fernuniversität Hagen (Theorie der Schule und des Unterrichts getragen. Die Projektleitung teile ich mit Horst Dichanz, Bardo Herzig und Johannes Magenheim. Der Fernstudiengang basiert auf Entwicklungen zur Lehrerausbildung im Bereich neuer Medien, wie sie im Rahmen der Initiative „Bildungswege in der Informationsgesellschaft (BIG“ erarbeitet wurden (vgl. u.a. Tulodziecki/Moll/Gallasch 2001. Die – 1995 begonnene – BIG-Initiative geht auf die Bertelsmann Stiftung und Heinz Nixdorf Stiftung zurück. Die jetzige Projektarbeit wird von der ,,e-nitiative.nrw.Netzwerk für Bildung“ gefördert.

  7. Ökonomie der Bandbreite: Evolutionär-ökonomische und kulturanthropologische Überlegungen zu Schnittstellen in Mensch-Maschine-Komplexen

    Directory of Open Access Journals (Sweden)

    Manuel Wäckerle

    2012-09-01

    Full Text Available In diesem Artikel diskutieren wir Bandbreite als kognitive, kulturelle und technische Regel, als eine Regel, die Informationsübertragung pro Zeiteinheit beschränkt. Wir verstehen Wirtschaft, Kultur und Gesellschaft als regelbasiertes evolvierendes System und geben der Bandbreite als generische Regel eine zentrale Rolle. Dabei untersuchen wir theoretisch die wechselwirkenden Prozesse der Ausbeutung und Ausweitung von Bandbreite in Mensch-Maschine-Komplexen. Des Weiteren verweisen wir auf die historische Dimension der digitalisierten Gesellschaft und diskutieren kulturelle Entwicklungen von evolvierten Prothesen. In diesem Zusammenhang formulieren wir eine Genealogie von Gleichheit und Freiheit im Netz. Abschließend betrachten wir zwei Beispiele von evolvierenden Prothesen: Das „Automated Trading“ und die Thematik des „Freundschaftskapitals bei Facebook“. Es zeigt sich, dass sich in beiden Beispielen, so unterschiedlich ihre Implikationen auf ersten Blick auch sind, systemisches Risiko auf ähnliche Art und Weise akkumuliert. Unser Artikel versucht einerseits wesentliche Aspekte der Ökonomie der Bandbreite aufzuzeigen, andererseits erste interdisziplinäre Denkschemata für ebendiese zu entwickeln.

  8. Airtight miniaturized chromatography: a safer method for radiopharmaceutical quality control

    International Nuclear Information System (INIS)

    Coupal, J.J.; Shih, W.J.; Ryo, U.Y.

    1988-01-01

    Miniaturized chromatography is widely used for quality control of radiopharmaceuticals. Recently, published chromatography procedures have illustrated or described chromatography chambers open to the air in use, suggesting that volatile toxic mobile phases are harmless to people in the vicinity. The authors describe the results of their search for an inexpensive closed chromatography chamber that can be used to derive safely the benefits from conventional miniaturized chromatography

  9. Pedological investigations regarding thermal, chemical and biological soil cleaning techniques. Final report for the period of investigation 1990 to 1994; Bodenkundliche Untersuchungen zu thermischen, chemischen und biologischen Bodenreinigungsverfahren. Schlussbericht fuer den Bearbeitungszeitraum 1990 bis 1994

    Energy Technology Data Exchange (ETDEWEB)

    Goetz, D; Bauske, B; Claussen, A; Glaeseker, W; Holz, C [Hamburg Univ. (Germany). Inst. fuer Bodenkunde

    1998-12-31

    The hazard potential of contaminated sites is evaluated by means of risk assessments. These can entail diverse measures being taken that may range from the mere observation of the site over protective and restricting measures, different encapsulations to ensure its safety as well as the shifting of contaminated matter, to the decontamination of the site (Council of Experts on Environmental Questions SRU, 1990). Of these measures, soil cleaning demands the greatest effort and should lastingly repair the damage without new damage being caused in another place. (orig.) [Deutsch] Das Gefaehrdungspotential kontaminierter Standorte wird ueber Gefaehrdungsabschaetzungen klassifiziert. Diese Einschaetzung kann zu verschiedenen Massnahmen fuehren. Sie reichen von der reinen Ueberwachung des Standortes ueber Schutz- und Beschraenkungsmassnahmen, Sicherung durch verschiedene Einkapselungen, Umlagerungsmassnahmen bis zur Dekontamination der verunreinigten Materialien (SRU, 1990). Die Bodenreinigung ist dabei die aufwendigste Massnahme und sollte zu einer endgueltigen Beseitigung des Schadens fuehren, ohne dass an anderer Stelle neue Lasten entstehen. (orig.)

  10. Stationary phases for superheated water chromatography

    International Nuclear Information System (INIS)

    Saha, Shikha

    2002-01-01

    This project focused on the comparison of conventional liquid chromatography and superheated water chromatography. It examined the differences in efficiency and retention of a range of different stationary phases. Alkyl aryl ketones and eight aromatic compounds were separated on PBD-zirconia, Xterra RP 18, Luna C 18 (2) and Oasis HLB columns using conventional LC and superheated water chromatography system. The retention indices were determined in the different eluents. On changing the organic component of the eluent from methanol to acetonitrile to superheated water considerable improvements were found in the peak shapes and column efficiencies on the PBD-zirconia and Oasis HLB columns. PS-DVB, PBD-zirconia and Xterra RP 18 columns have been used in efficiency studies. It was found that simply elevating the column temperature did not increase the efficiency of a separation in superheated water chromatography. The efficiency depended on flow rate, injection volume and also mobile phase preheating system. Although high efficiencies were not achieved with superheated water on PS-DVB and Xterra RP 18 columns, a higher efficiency was achieved on a PBD-zirconia column with superheated water than with 25-35% ACN at room temperature. The proposed theoretical increases in u opt were measured on three columns using superheated water as the mobile phase. The application of the superheated water chromatographic method to the separation of the pungent constituents of ginger by superheated water chromatography-NMR coupling system was studied. The coupling of superheated water chromatography using deuterium oxide to NMR spectroscopy for the separation of dry ginger extract was successful, although the NMR sensitivity in on-line mode coupling system was low. However, four compounds were identified in the ginger extract by stop-flow mode on superheated water chromatography-UV-NMR detection system. (author)

  11. Analytic studies of test problems of laser welds in consideration of tolerated hardness peaks. Final report; Analytische Untersuchungen der Pruefproblematik an laserstrahlgeschweissten Verbindungen unter besonderer Beruecksichtigung der zu tolerierenden Haertespitzen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    Test methods for laser welds were investigated under the following aspects: 1. Setting a defined cracking path by changing the test piece geometry - is it possible and does it make sense? 2. Can the results obtained with changed test piece geometries be applied to standard test pieces? 3. Is crack displacement typical of small test pieces, or is it observable in real components as well? 4. Should the cracking path be predefined at all if it results in 'non-realistic' fractures? The focus was on notched bar impact tests. [German] Ziel des Forschungsvorhabens war die Ermittlung geeigneter Pruefverfahren zur Charakterisierung einer Laserstrahlschweissnaht. Folgende Fragen sollten geklaert werden: 1. Ist es moeglich und sinnvoll, mit einer Aenderung der Probengeometrie (z.B. Einbringen von Seitenkerben) den Rissverlauf in das aufgehaertete Laserschweissgut zu zwingen? 2. Welche Aussagen liefern uns die Ergebnisse dieser abgeaenderten Probengeometrie und inwiefern sind diese vor allen Dingen beim Kerbschlagbiegeversuch auf die Ergebnisse der standardisierten Probengeometrie zu uebertragen? 3. Ist das Rissauswandern ein nur fuer Kleinproben typisches Verhalten oder wird es auch bei realen Bauteilen beobachtet? 4. Ist es sinnvoll, den Rissverlauf in die sproede, aufgehaertete Schweissnaht zu zwingen, obwohl daraus ein 'realitaetsfernes' Bruchverhalten resultiert? Dabei wurde der Schwerpunkt auf die Untersuchung des Kerbschlagbiegeversuches gelegt. (orig.)

  12. Reimbursement for electricity supplied to the grid and the phaseout of franchise agreements. A critical review of recent developments of the law; Einspeiseverguetung und Auslaufen der Konzessionsvertraege - eine kritische Studie zu neueren Rechtsentwicklungen

    Energy Technology Data Exchange (ETDEWEB)

    Jackel, G. [Energieversorgung Schwaben AG, Stuttgart (Germany)

    1994-12-31

    Allowing more competition in all sectors of the economy is an acceptable strategy if resulting in social benefits, i.e. if profits are passed on to the citizens and consumers. If, however, such a liberalisation or deregulation ends up in a concentration of power by commercial enterprises to the disadvantage of consumers who have to pay higher electricity rates, or jeopardizes the reliability of power supply, such actionism on the part of the legislature may well adversely affect public welfare. This applies likewise to minor regulation, as is the Act on Compulsory Acceptance of Electricity From Renewable Energy Sources,(SEG), which puts an unproportionate load on the shoulders of the public utilities in order to satisfy interests of environmental policy. The paper explains these statements by examining effects expected to result from the SEG, and by referring to problems involved in the phaseout of franchise agreements. (orig./HSCH) [Deutsch] Die Einfuehrung von mehr Wettbewerb in allen Bereichen der Wirtschaft ist zu begruessen, wenn sich volkswirtschaftlich positive Entwicklungen einstellen, d.h. der Buerger, der Kunde davon profitiert. Wenn liberalisierende Eingriffe letztlich aber zu hoeheren Strompreisen oder zu geringerer Versorgungssicherheit fuehren, bei gleichzeitigem Konzentrationsschub in der Unternehmensstruktur, dann kann das Gemeinwohl durch gesetzgeberischen Aktionismus leicht Schaden nehmen. Dies gilt auch fuer Regulierungen minderer Art wie beim Stromeinspeisungsgesetzt (SEG), das die deutschen Energieversorgungsunternehmen (EVU) aus umweltpolitischen Erwaegungen weiter einseitig belastet. (orig./HSCH)

  13. Laboratory and field based evaluation of chromatography ...

    Science.gov (United States)

    The Monitor for AeRosols and GAses in ambient air (MARGA) is an on-line ion-chromatography-based instrument designed for speciation of the inorganic gas and aerosol ammonium-nitrate-sulfate system. Previous work to characterize the performance of the MARGA has been primarily based on field comparison to other measurement methods to evaluate accuracy. While such studies are useful, the underlying reasons for disagreement among methods are not always clear. This study examines aspects of MARGA accuracy and precision specifically related to automated chromatography analysis. Using laboratory standards, analytical accuracy, precision, and method detection limits derived from the MARGA chromatography software are compared to an alternative software package (Chromeleon, Thermo Scientific Dionex). Field measurements are used to further evaluate instrument performance, including the MARGA’s use of an internal LiBr standard to control accuracy. Using gas/aerosol ratios and aerosol neutralization state as a case study, the impact of chromatography on measurement error is assessed. The new generation of on-line chromatography-based gas and particle measurement systems have many advantages, including simultaneous analysis of multiple pollutants. The Monitor for Aerosols and Gases in Ambient Air (MARGA) is such an instrument that is used in North America, Europe, and Asia for atmospheric process studies as well as routine monitoring. While the instrument has been evaluat

  14. Comprehensive two-dimensional liquid chromatography: Ion chromatography × reversed-phase liquid chromatography for separation of low-molar-mass organic acids

    NARCIS (Netherlands)

    Brudin, S.S.; Shellie, R.A.; Haddad, P.R.; Schoenmakers, P.J.

    2010-01-01

    In the work presented here a novel approach to comprehensive two-dimensional liquid chromatography is evaluated. Ion chromatography is chosen for the first-dimension separation and reversed-phase liquid chromatography is chosen for the second-dimension separation mode. The coupling of these modes is

  15. Alte und neue Problemaspekte der Figur. Zu einem medienübergreifenden Versuch einer Begriffsbestimmung der Figur [Jens Eder / Fotis Jannidis / Ralf Schneider (Hg.: Characters in Fic­tional Worlds. Understanding Imaginary Beings in Literature, Film, and Other Media. Berlin 2010

    Directory of Open Access Journals (Sweden)

    Carmen Lăcan

    2012-12-01

    Full Text Available Rezension zu / Review of:Jens Eder / Fotis Jannidis / Ralf Schneider (Hg.: Characters in Fic­tional Worlds. Understanding Imaginary Beings in Literature, Film, and Other Media. Berlin 2010

  16. Diagonal chromatography to study plant protein modifications.

    Science.gov (United States)

    Walton, Alan; Tsiatsiani, Liana; Jacques, Silke; Stes, Elisabeth; Messens, Joris; Van Breusegem, Frank; Goormachtig, Sofie; Gevaert, Kris

    2016-08-01

    An interesting asset of diagonal chromatography, which we have introduced for contemporary proteome research, is its high versatility concerning proteomic applications. Indeed, the peptide modification or sorting step that is required between consecutive peptide separations can easily be altered and thereby allows for the enrichment of specific, though different types of peptides. Here, we focus on the application of diagonal chromatography for the study of modifications of plant proteins. In particular, we show how diagonal chromatography allows for studying proteins processed by proteases, protein ubiquitination, and the oxidation of protein-bound methionines. We discuss the actual sorting steps needed for each of these applications and the obtained results. This article is part of a Special Issue entitled: Plant Proteomics--a bridge between fundamental processes and crop production, edited by Dr. Hans-Peter Mock. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Inert carriers for column extraction chromatography

    International Nuclear Information System (INIS)

    Katykhin, G.S.

    1978-01-01

    Inert carriers used in column extraction chromatography are reviewed. Such carriers are devided into two large groups: hydrophilic carriers which possess high surface energy and are well wetted only with strongly polar liquids (kieselguhrs, silica gels, glasses, cellulose, Al 2 O 3 ) and water-repellent carriers which possess low surface energy and are well wetted with various organic solvents (polyethylene, polytetrafluorethylene polytrifluorochlorethylene). Properties of various carriers are presented: structure, chemical and radiation stability, adsorption properties, extracting agent capacity. The effect of structure and sizes of particles on the efficiency of chromatography columns is considered. Ways of immovable phase deposition on the carrier and the latter's regeneration. Peculiarities of column packing for preparative and continuous chromatography are discussed

  18. Liquid chromatography detection unit, system, and method

    Science.gov (United States)

    Derenzo, Stephen E.; Moses, William W.

    2015-10-27

    An embodiment of a liquid chromatography detection unit includes a fluid channel and a radiation detector. The radiation detector is operable to image a distribution of a radiolabeled compound as the distribution travels along the fluid channel. An embodiment of a liquid chromatography system includes an injector, a separation column, and a radiation detector. The injector is operable to inject a sample that includes a radiolabeled compound into a solvent stream. The position sensitive radiation detector is operable to image a distribution of the radiolabeled compound as the distribution travels along a fluid channel. An embodiment of a method of liquid chromatography includes injecting a sample that comprises radiolabeled compounds into a solvent. The radiolabeled compounds are then separated. A position sensitive radiation detector is employed to image distributions of the radiolabeled compounds as the radiolabeled compounds travel along a fluid channel.

  19. Electrochemical Detectors in HPLC and Ion Chromatography.

    Science.gov (United States)

    Horvai, George; Pungor, ErnÕ

    1989-01-01

    Back in 1952, the renowned Polish electrochemist Wiktor Kemula introduced chromato-polarography, 1 i.e., polaro-graphic detection for liquid chromatography. This technique continued to develop slowly until the early 1970s (for a review see Reference 2) when modem high-performance liquid chromatography (HPLC) emerged. This new, highly efficient chromatographc method could only be. used with detectors ensuring low dispersion. It was not easy to modify the dropping mercury electrode cells to satisfy this requirement. However, at the same time, electroanalytical chemists, who already had much experience in using carbon-based electrodes for oxidative detection in flow analysis, put forward the idea of oxidative amperometric detection in liquid chromatography. 3,4 In this technique, solid or quasi-solid (paste) electrodes were used and this made possible the construction of miniaturized cells with just a few microliter volume.

  20. ILK statement on the recommendations by the working group on procedures for the selection of repository sites; ILK-Stellungnahme zu den Empfehlungen des Arbeitskreises Auswahlverfahren Endlagerstandorte Internationale (AkEnd)

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2003-11-01

    The Working Group on Procedures for the Selection of Repository Sites (AkEnd) had been appointed by the German Federal Ministry for the Environment (BMU) to develop procedures and criteria for the search for, and selection of, a repository site for all kinds of radioactive waste in deep geologic formations in Germany. ILK in principle welcomes the attempt on the part of AkEnd to develop a systematic procedure. On the other hand, ILK considers the two constraints imposed by BMU inappropriate: AkEnd was not to take into account the two existing sites of Konrad and Gorleben and, instead, work from a so-called white map of Germany. ILK recommends to perform a comprehensive safety analysis of Gorleben and define a selection procedure including the facts about Gorleben and, in addition, to commission the Konrad repository as soon as possible. The one-repository concept established as a precondition by BMU greatly restricts the selection procedure. There are no technical or scientific reasons for such concept. ILK recommends to plan for separate repositories, which would also correspond to international practice. The geoscientific criteria proposed by AkEnd should be examined and revised. With respect to the site selection procedure proposed, ILK feels that procedure is unable to define a targeted approach. Great importance must be attributed to public participation. The final site selection must be made under the responsibility of the government or the parliament. (orig.) [German] Der Arbeitskreis Auswahlverfahren Endlagerstandorte (AkEnd) hat Ende 2002 seine Empfehlungen vorgestellt. Der AkEnd war vom Bundesumweltministerium (BMU) berufen worden, um Verfahren und Kriterien fuer die Suche und die Auswahl eines Endlagerstandortes fuer alle Arten radioaktiver Abfaelle in tiefen geologischen Formationen in Deutschland zu entwickeln. Die ILK begruesst grundsaetzlich den Versuch des AkEnd, ein systematisches Verfahren zu entwickeln. Allerdings haelt die ILK die beiden vom BMU

  1. Validated High Performance Liquid Chromatography Method for ...

    African Journals Online (AJOL)

    Purpose: To develop a simple, rapid and sensitive high performance liquid chromatography (HPLC) method for the determination of cefadroxil monohydrate in human plasma. Methods: Schimadzu HPLC with LC solution software was used with Waters Spherisorb, C18 (5 μm, 150mm × 4.5mm) column. The mobile phase ...

  2. Electronic spectral detection in liquid chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Biggs, Wilton R. [ChevronTexaco Chemical Co., P. O. Box 1627, Richmond, CA 94802 (United States); Fetzer, John C. [Fetzpahs Consulting, 1283 Santa Fe St., Hercules, CA 94547 (United States)

    2002-07-01

    The use of absorbance and fluorescence spectrometers as liquid chromatography detectors is described. These detectors offer sensitive detection that can also identify the peaks in a chromatogram. Many examples, mainly from the separation of polycyclic aromatic hydrocarbons, are given that show the selectivity and usefulness of these detectors. (orig.)

  3. Ionic liquid stationary phases for gas chromatography.

    Science.gov (United States)

    Poole, Colin F; Poole, Salwa K

    2011-04-01

    This article provides a summary of the development of ionic liquids as stationary phases for gas chromatography beginning with early work on packed columns that established details of the retention mechanism and established working methods to characterize selectivity differences compared with molecular stationary phases through the modern development of multi-centered cation and cross-linked ionic liquids for high-temperature applications in capillary gas chromatography. Since there are many reviews on ionic liquids dealing with all aspects of their chemical and physical properties, the emphasis in this article is placed on the role of gas chromatography played in the design of ionic liquids of low melting point, high thermal stability, high viscosity, and variable selectivity for separations. Ionic liquids provide unprecedented opportunities for extending the selectivity range and temperature-operating range of columns for gas chromatography, an area of separation science that has otherwise been almost stagnant for over a decade. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Gas chromatography at the Tritium Laboratory Karlsruhe

    International Nuclear Information System (INIS)

    Laesser, R.; Gruenhagen, S.

    2003-08-01

    Among the analytical techniques (mass spectrometry, laser Raman spectroscopy, gas chromatography, use of ionisation chambers) employed at the Tritium Laboratory Karlsruhe (TLK), gas chromatography plays a prominent role. The main reasons for that are the simplicity of the gas chromatographic separation process, the small space required for the equipment, the low investment costs in comparison to other methods, the robustness of the equipment, the simple and straightforward analysis and the fact that all gas species of interest (with the exception of water) can easily be detected by gas chromatographic means. The conventional gas chromatographs GC1 and GC2 used in the Tritium Measurement Techniques (TMT) System of the TLK and the gas chromatograph GC3 of the experiment CAPER are presented in detail, by discussing their flow diagrams, their major components, the chromatograms measured by means of various detectors, shortcomings and possible improvements. One of the main disadvantages of the conventional gas chromatography is the long retention times required for the analysis of hydrogen gas mixtures. To overcome this disadvantage, micro gas chromatography for hydrogen analysis was developed. Reduction of the retention times by one order of magnitude was achieved. (orig.)

  5. Instrument platforms for nano liquid chromatography

    Czech Academy of Sciences Publication Activity Database

    Šesták, Jozef; Moravcová, Dana; Kahle, Vladislav

    2015-01-01

    Roč. 1421, NOV (2015), s. 2-17 ISSN 0021-9673 R&D Projects: GA MV VG20112015021 Institutional support: RVO:68081715 Keywords : nano liquid chromatography * splitless gradient generation * nano LC platforms Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 3.926, year: 2015 http://hdl.handle.net/11104/0250900

  6. Instrument platforms for nano liquid chromatography.

    Science.gov (United States)

    Šesták, Jozef; Moravcová, Dana; Kahle, Vladislav

    2015-11-20

    The history of liquid chromatography started more than a century ago and miniaturization and automation are two leading trends in this field. Nanocolumn liquid chromatography (nano LC) and largely synonymous capillary liquid chromatography (capillary LC) are the most recent results of this process where miniaturization of column dimensions and sorbent particle size play crucial role. Very interesting results achieved in the research of extremely miniaturized LC columns at the end of the last century lacked distinctive raison d'être and only advances in mass spectrometry brought a real breakthrough. Configuration of nano LC-electrospray ionization mass spectrometry (LC-ESI-MS) has become a basic tool in bioanalytical chemistry, especially in proteomics. This review discusses and summarizes past and current trends in the realization of nano liquid chromatography (nano LC) platforms. Special attention is given to the mobile phase delivery under nanoflow rates (isocratic, gradient) and sample injection to the nanocolumn. Available detection techniques applied in nano LC separations are also briefly discussed. We followed up the key themes from the original scientific reports over gradual improvements up to the contemporary commercial solutions. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. Ultra Performance Liquid Chromatography Tandem Mass ...

    African Journals Online (AJOL)

    NICOLAAS

    drugs alone.16. After a single oral dose of 120–800 mg of NTB in healthy sub- jects in a fasting state the peak plasma NTB concentration (tmax) was found to be 4–7 h, with a half-life of approximately 9–17 h.17 ... performance liquid chromatography mass spectrometry/mass .... to the likely biological plasma constituents.

  8. Analytical biotechnology: Capillary electrophoresis and chromatography

    International Nuclear Information System (INIS)

    Horvath, C.; Nikelly, J.G.

    1990-01-01

    The papers describe the separation, characterization, and equipment required for the electrophoresis or chromatography of cyclic nucleotides, pharmaceuticals, therapeutic proteins, recombinant DNA products, pheromones, peptides, and other biological materials. One paper, On-column radioisotope detection for capillary electrophoresis, has been indexed separately for inclusion on the data base

  9. Bibliography of extraction chromatography, ch. 15

    International Nuclear Information System (INIS)

    Eschrich, H.; Drent, W.

    1975-01-01

    A bibliography of reviews, books and publications dealing with laminar extraction chromatography is given. A tabular survey of experimental data on column extraction chromatographic investigations, stationary phases and support materials is presented. An author index and corporative author index are included. This bibliography refers to various publications relevant to nuclear interest

  10. Gas chromatography in blood carbon monoxide monitoring

    Energy Technology Data Exchange (ETDEWEB)

    Drasche, H.; Funk, L.; Herbolsheimer, R.

    1975-05-01

    A description of a gas chromatography method for monitoring blood carboxyhaemoglobin (HbCO) levels in a very small quantity (100 mcl) of capillary blood: reagents and apparatus, procedures, calculation of results. To calculate HbCO content, an aliquot portion of water-diluted blood is saturated with CO; this saturation obviates the need to determine the haemoglobin or iron blood levels.

  11. Loading Effects on Resolution in Thin Layer Chromatography and ...

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 10; Issue 11. Loading Effects on Resolution in Thin Layer Chromatography and Paper Chromatography. K Girigowda V H Mulimani. Classroom Volume 10 Issue 11 November 2005 pp 79-84 ...

  12. Separation of Chloroplast Pigments Using Reverse Phase Chromatography.

    Science.gov (United States)

    Reese, R. Neil

    1997-01-01

    Presents a protocol that uses reverse phase chromatography for the separation of chloroplast pigments. Provides a simple and relatively safe procedure for use in teaching laboratories. Discusses pigment extraction, chromatography, results, and advantages of the process. (JRH)

  13. High Performance Liquid Chromatography Experiments to Undergraduate Laboratories

    Science.gov (United States)

    Kissinger, Peter T.; And Others

    1977-01-01

    Reviews the principles of liquid chromatography with electrochemical detection (LCEC), an analytical technique that incorporates the advantages of both liquids chromatography and electrochemistry. Also suggests laboratory experiments using this technique. (MLH)

  14. Development of a high performance liquid chromatography method ...

    African Journals Online (AJOL)

    Development of a high performance liquid chromatography method for simultaneous ... Purpose: To develop and validate a new low-cost high performance liquid chromatography (HPLC) method for ..... Several papers have reported the use of ...

  15. Optimizing separations in online comprehensive two‐dimensional liquid chromatography

    OpenAIRE

    Pirok, Bob W.J.; Gargano, Andrea F.G.; Schoenmakers, Peter J.

    2017-01-01

    Abstract Online comprehensive two‐dimensional liquid chromatography has become an attractive option for the analysis of complex nonvolatile samples found in various fields (e.g. environmental studies, food, life, and polymer sciences). Two‐dimensional liquid chromatography complements the highly popular hyphenated systems that combine liquid chromatography with mass spectrometry. Two‐dimensional liquid chromatography is also applied to the analysis of samples that are not compatible with mass...

  16. Eine zu Recht vergessene Vergangenheit?

    Directory of Open Access Journals (Sweden)

    Oliver Lepsius

    2013-01-01

    Full Text Available Review of: David M. Rabban, Law’s History. American Legal Thought and the Transatlantic Turn to History, Cambridge: Cambridge University Press 2013, XVI, 564 S., ISBN 978-0-521-76191-8

  17. Notizen zu altpr. mosla, moska

    Directory of Open Access Journals (Sweden)

    Bernd Gliwa

    2011-11-01

    Full Text Available Pastabos dėl pr. mosla, moskaSantraukaŽodžių pr. mosla – v.v.a. leimat (GrG73, moska – leimet (GrA85, Moska – Leimet, Viscus (GrF86, išskyrus lo. viscus, reikšmės nėra aiškios, o pastarasis yra daugiareikšmis. Svarstoma keletas prūsų kalbos rūpimo žodžio kilmės variantų, manant, kad lo. viscus „amalas, klijai iš amalo, spąstai paukš­čiams su klijais“ teisingai perteikia pr. mosla.1. Jei žodis įvardytų masalą, ypač su klijais smulkiems paukščiams gaudyti (plg. v.v.a. lîm–ruote, tada pr. mosla lygintinas su lie. mãsalas, mãsinti, kur pr. šaknyje būtų kaip Noye (Gr. = nage (E 145, pig. lie. nagà. Darybiškai pr. mosla gretintinas su lie. mãsalas kaip lie. dẽglas – su dẽgalas, plerplà – su pler̃palas. Beje, nerasta jokių duomenų, kad baltai būtų taip medžioję.2. Kadangi vok. Mistel „amalas“ galbūt yra vedinys iš Mist „mėšlas“, ieškota mosla sąsajos su lie. mė́šlas, mė́žti. Galima pastarojo žodžio giminystė su lie. mỹžti rodytų šaknies apofonijos i eilę, kur laipsnis ō nelauktinas. Variantas atmetamas.3. Amalas yra krūmu ant medžių augantis parazitas. Pavadinimai įvardija jo formą, pvz.: lie. laumės šluota, vok. dial. Kraigennest „varnos lizdas“. Vok. dial. Wispe, Wespe, atrodo, irgi perteikia augalo formą, plg. norv. visp „krūmelis“, šved. visp „virbų maišiklis“ ir vespelten, vispelten „ama­las“. Šiuo atžvilgiu pr. žodį galima lyginti su lie. mastas „mazgas, juosta“ , mãzgas „t.p.“, užsimègzti „pradėti augti (apie tik apvaisintą vaisių“, – tada pr. mosla [masla] būtų *“kas susimazgojęs; kas auga kaip mazgas“.4. Dažnai pr. (Gr šaknyje atitinka lie. u, pvz.: pr. Songos – lie. šunį, šuo. Tad svarstytina pr. mosla sąsaja su lie. mus-: 2mùsti (muñta, mùto „verdant tirštėti...“, mū̃sas „plėvė ant skysčio; vario oksidas; pelėsiai“. Šalia ū esama ir lyčių su u: musójai bei mūsojaĩ „plėvė ant rūgusio pieno, alaus, uogienės...“. Kadangi ide. *meu– vediniai reiškia tiek „maudyti...“, tiek „pelėti...“, trūksta bendros semos. Suponuotina pirmykštė ide. *meu– reikšmė „(plono sluoksnio susidarymas“ > *“vandens sluoks­nis ant kūno“ > *“maudytis“: lie. máudyti. Tam tikras sluoksnis gali būti ir prakaitas (plg. lie. sumutęs „sušilęs = suprakaitavęs“ arklys, rasa: mūsalas „rasa ant lango“. Šiame kontekste pr. mosla < *mus-sla  arba *mu-sla gali būti nomen instr. „tirštiklis, fermentas“, išskiriantis amalo vartojimą, kaip lie. tirkšlis „amalas“ < nom. instr. *“tirštiklis“.5. Kaip (4, tik semantinė raida kitaip suvokiama: mosla *“amalas“ < *“kas tankina medžio lają, medį dengia“.

  18. Comparison of ultra high performance supercritical fluid chromatography, ultra high performance liquid chromatography, and gas chromatography for the separation of synthetic cathinones.

    Science.gov (United States)

    Carnes, Stephanie; O'Brien, Stacey; Szewczak, Angelica; Tremeau-Cayel, Lauriane; Rowe, Walter F; McCord, Bruce; Lurie, Ira S

    2017-09-01

    A comparison of ultra high performance supercritical fluid chromatography, ultra high performance liquid chromatography, and gas chromatography for the separation of synthetic cathinones has been conducted. Nine different mixtures of bath salts were analyzed in this study. The three different chromatographic techniques were examined using a general set of controlled synthetic cathinones as well as a variety of other synthetic cathinones that exist as positional isomers. Overall 35 different synthetic cathinones were analyzed. A variety of column types and chromatographic modes were examined for developing each separation. For the ultra high performance supercritical fluid chromatography separations, analyses were performed using a series of Torus and Trefoil columns with either ammonium formate or ammonium hydroxide as additives, and methanol, ethanol or isopropanol organic solvents as modifiers. Ultra high performance liquid chromatographic separations were performed in both reversed phase and hydrophilic interaction chromatographic modes using SPP C18 and SPP HILIC columns. Gas chromatography separations were performed using an Elite-5MS capillary column. The orthogonality of ultra high performance supercritical fluid chromatography, ultra high performance liquid chromatography, and gas chromatography was examined using principal component analysis. For the best overall separation of synthetic cathinones, the use of ultra high performance supercritical fluid chromatography in combination with gas chromatography is recommended. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. High perfomance liquid chromatography in pharmaceutical analyses.

    Science.gov (United States)

    Nikolin, Branko; Imamović, Belma; Medanhodzić-Vuk, Saira; Sober, Miroslav

    2004-05-01

    In testing the pre-sale procedure the marketing of drugs and their control in the last ten years, high performance liquid chromatography replaced numerous spectroscopic methods and gas chromatography in the quantitative and qualitative analysis. In the first period of HPLC application it was thought that it would become a complementary method of gas chromatography, however, today it has nearly completely replaced gas chromatography in pharmaceutical analysis. The application of the liquid mobile phase with the possibility of transformation of mobilized polarity during chromatography and all other modifications of mobile phase depending upon the characteristics of substance which are being tested, is a great advantage in the process of separation in comparison to other methods. The greater choice of stationary phase is the next factor which enables realization of good separation. The separation line is connected to specific and sensitive detector systems, spectrafluorimeter, diode detector, electrochemical detector as other hyphernated systems HPLC-MS and HPLC-NMR, are the basic elements on which is based such wide and effective application of the HPLC method. The purpose high performance liquid chromatography (HPLC) analysis of any drugs is to confirm the identity of a drug and provide quantitative results and also to monitor the progress of the therapy of a disease.1) Measuring presented on the Fig. 1. is chromatogram obtained for the plasma of depressed patients 12 h before oral administration of dexamethasone. It may also be used to further our understanding of the normal and disease process in the human body trough biomedical and therapeutically research during investigation before of the drugs registration. The analyses of drugs and metabolites in biological fluids, particularly plasma, serum or urine is one of the most demanding but one of the most common uses of high performance of liquid chromatography. Blood, plasma or serum contains numerous endogenous

  20. Advances in chromatography of the rare earth elements (review)

    International Nuclear Information System (INIS)

    Oguma, Koichi; Kuroda, Rokuro; Shimizu, Tsuneo.

    1995-01-01

    A review is presented which covers liquid chromatography, gas chromatography, and related techniques. This article intends to describe the chromatographic methods playing an important role in the separation of the rare earth elements. Special attention is paid to the usefulness of various types of liquid chromatography which enable the complete mutual separation of the rare earth elements. Applications are also discussed. (author) 161 refs

  1. High performance liquid chromatography in pharmaceutical analyses

    Directory of Open Access Journals (Sweden)

    Branko Nikolin

    2004-05-01

    Full Text Available In testing the pre-sale procedure the marketing of drugs and their control in the last ten years, high performance liquid chromatographyreplaced numerous spectroscopic methods and gas chromatography in the quantitative and qualitative analysis. In the first period of HPLC application it was thought that it would become a complementary method of gas chromatography, however, today it has nearly completely replaced gas chromatography in pharmaceutical analysis. The application of the liquid mobile phase with the possibility of transformation of mobilized polarity during chromatography and all other modifications of mobile phase depending upon the characteristics of substance which are being tested, is a great advantage in the process of separation in comparison to other methods. The greater choice of stationary phase is the next factor which enables realization of good separation. The separation line is connected to specific and sensitive detector systems, spectrafluorimeter, diode detector, electrochemical detector as other hyphernated systems HPLC-MS and HPLC-NMR, are the basic elements on which is based such wide and effective application of the HPLC method. The purpose high performance liquid chromatography(HPLC analysis of any drugs is to confirm the identity of a drug and provide quantitative results and also to monitor the progress of the therapy of a disease.1 Measuring presented on the Fig. 1. is chromatogram obtained for the plasma of depressed patients 12 h before oral administration of dexamethasone. It may also be used to further our understanding of the normal and disease process in the human body trough biomedical and therapeutically research during investigation before of the drugs registration. The analyses of drugs and metabolites in biological fluids, particularly plasma, serum or urine is one of the most demanding but one of the most common uses of high performance of liquid chromatography. Blood, plasma or

  2. Dressing the Black Body. Mode, Hairstyle und Schwarzsein in den USA – von den 1970er-Jahren bis zu Barack Obama

    Directory of Open Access Journals (Sweden)

    Philipp Dorestal

    2017-08-01

    Full Text Available Conventional forms of African American activism and organization in the second half of the twentieth century have been the subject of academic research for quite some time, but less attention has been devoted to symbolic and body political forms of intervention. Looking at the Afro hairstyle and hip hop culture, this article explores how African American identity has been reflected through Style Politics. It outlines the history of African American hairstyles from the 1970s until the 2000s and identifies elements of popular culture such as Blaxploitation movies and rap music as constitutive of African American fashion. The question of ›respectability‹ that surfaced in the context of racist discourses about black women will be examined through the example of First Lady Michelle Obama, who rose to fame as a fashion icon. In conclusion, the article discusses the case of Trayvon Martin, an African American teenager who was killed in 2012 while wearing a hoodie, making the garment once again an object of contention and a symbol of protest. * * * Klassische Formen des Aktivismus und der Organisierung von African Americans in der zweiten Hälfte des 20. Jahrhunderts sind schon seit geraumer Zeit Gegenstand wissenschaftlicher Studien. In der bisherigen Forschung weniger gewürdigt wurden jedoch symbolische und körperpolitische Interventionsformen. Dieser Aufsatz geht anhand der Afro-Frisur und der durch die Hip-Hop-Kultur inspirierten Mode der Frage nach, wie afroamerikanische Identität über Style Politics verhandelt wurde. Skizziert wird die Geschichte afroamerikanischer Hairstyles von den 1970er- bis zu den 2000er-Jahren. Weiterhin werden Elemente der Populärkultur wie das Blaxploitation-Kino oder die seit den 1980er-Jahren verbreitete Rap-Musik als stilbildend für afroamerikanische Modephänomene identifiziert. Die Frage der »Respektabilität«, die im Kontext rassistischer Diskurse über schwarze Frauen auftauchte, wird anhand von Michelle

  3. Image quality of paper prints compared to film copies in CT investigations; Bildqualitaet von Papierausdrucken zur Befunddokumentation im Vergleich zu Laserfilmen bei MSCT-Untersuchungen

    Energy Technology Data Exchange (ETDEWEB)

    Bley, T.; Burger, D.; Ghanem, N.; Thuerl, C.; Saueressig, U.; Kotter, E.; Langer, M. [Abt. Roentgendiagnostik, Radiologische Klinik, Universitaetsklinikum Freiburg (Germany)

    2002-04-01

    Purpose: Evaluation of image quality of paper prints in routine CT investigations. Method: The image quality of paper prints from 104 CT scans of daily routine investigations was analysed by three observer three observers according to a five-point ranking scale for contrast, grey level, spatial resolution, and subjective confidence in correct evaluation. Each study was rated ''acceptable'' or ''not acceptable for documentation''. Results: In 312 ratings the mean grade for contrast was 2.7, for grey levels 3.2, and for spatial resolution 3.3. Grades ranged from 1 = very good to 5 = insufficient. Subjective confidence in correct evaluation was rated as ''certain'' in 77.4%, ''likely'' in 18.4% and ''impossible to evaluate'' in 4.2% of cases. 93.7% of printed CT images were rated ''acceptable for documentation''. Conclusion: Image quality of paper prints does not reach the quality of laser films concerning its contrast, grey levels, and spatial resolution. Nevertheless, paper prints are acceptable for documentation of findings in most CT investigations. A high confidence in correct evaluation was found. Limitations are found in documentation of small coin lesions of the lung and ischemic lesions of the neurocranium. (orig.) [German] Ziel: Qualitaetsbewertung von Papierausdrucken zur Bilddokumentation von Routine-CT-Untersuchungen. Material und Methoden: Bei 104 Routine-CT-Untersuchungen werteten drei Untersucher (insgesamt 312 Auswertungen) die Bildqualitaet von Papierausdrucken im Vergleich zu Laserfilmen bezueglich Kontrast, Grauwerten und Aufloesung sowie ''persoenlicher Befundsicherheit'' (Skala 1 - 5) aus. Abschliessend wurde entschieden, ob der Papierausdruck fuer die Befunddokumentation ausreiche. Ergebnisse: Mit Bewertungen zwischen 1 fuer ''sehr gut'' und 5 fuer &apos

  4. Studies on the turnover and properties of dump groundwaters in brown coal mining areas and possibilities of influencing them. Final report; Untersuchungen zum Stoffumsatz und zur Beschaffenheit der Kippengrundwaesser in Braunkohlebergbaugebieten und Moeglichkeiten zu deren Beeinflussung. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Katzur, J.; Liebner, F.; Liebner, C.; Welzel, H.P.; Hettrich, K.

    2001-07-01

    Only 6 days after the increase in pH with water saturation the concentration of iron in the pore water of the T1 sediment had dropped to a low value, 70% of which was accounted for by Fe(II). This is probably due to the almost complete precipitation of Fe(III) caused by the high pH values. Compared with the untreated variant the lowering of pH in the further course of the experiment led to a slight Fe(III) mobilisation and hence, through interaction with other reduction processes, to erratic changes in the share of Fe(II) in total Fe. Although this sediment showed a relatively low iron concentration a comparison showed that higher pH values and incubation temperatures have an influence on microbial iron reduction. [German] Im Porenwasser des Sedimentes T1 lag Eisen bereits 6 Tage nach pH-Erhoehung und Wassersaettigung nur noch in geringer Konzentration und zu ueber 70% als Fe(II) vor. Dies ist vermutlich in erster Linie auf die nahezu vollstaendige Ausfaellung von Fe(III) infolge der hohen pH-Werte zurueckzufuehren. Im weiteren Versuchsverlauf fuehrte das Absinken der pH-Werte zu einer im Vergleich zur unbehandelten Variante geringfuegigen Fe(III)-Mobilisierung und damit in Wechselwirkung mit ablaufenden Reduktionsprozessen zu unstetigen Veraenderungen in den Anteilen Fe(II) an Fe{sub ges}. Obwohl in diesem Sediment nur vergleichsweise niedrige Eisenkonzentrationen vorliegen, ergab ein Vergleich, dass hoehere pH-Werte und Inkubationtemperaturen die mikrobielle Eisenreduktion beeinflussen. (orig.)

  5. Research activities of MPA, Stuttgart University, for enhanced safety and reliability of components under complex load; Forschungsaktivitaeten der MPA Universitaet Stuttgart zu Erhoehung der Sicherheit und Zuverlaessigkeit komplex beanspruchter Bauteile

    Energy Technology Data Exchange (ETDEWEB)

    Herter, K.H.; Roos, E.; Schuler, X.; Maile, K. [Materialpruefungsanstalt (MPA), Univ. Stuttgart, Stuttgart (Germany)

    2004-07-01

    MPA research activities focus on fracture prevention and on the development of a generally applicable method of component integrity testing which, independent of the safety relevance of the components involved, is also part of ageing management. (orig.) [German] Die Forschungsaktivitaeten an der MPA im Hinblick auf die Erhoehung der Sicherheit und Zuverlaessigkeit komplex beanspruchter Bauteile orientieren sich an den Grundgedanken des Basissicherheitskonzepts (Prinzip des Bruchausschlusses). Zielsetzung war dabei die Entwicklung eines allgemein anwendbaren Nachweisverfahrens zur Komponentenintegritaet, das, abhaengig von der sicherheitstechnischen Relevanz der zu betrachtenden Komponenten, auch Bestandteil des Alterungsmanagements ist. (orig.)

  6. Rezension zu: Sabine Hark, Paula-Irene Villa (Hg.: Anti-Genderismus. Sexualität und Geschlecht als Schauplätze aktueller politischer Auseinandersetzungen. Bielefeld: transcript Verlag 2015.

    Directory of Open Access Journals (Sweden)

    Christian Hammermann

    2017-05-01

    Full Text Available Der von Sabine Hark und Paula-Irene Villa herausgegebene Sammelband analysiert die öffentlichen Angriffe auf Gender Studies und Gender Mainstreaming, die unter dem Begriff Anti-Genderismus auftreten und verhandelt werden. Die einzelnen Beiträge untersuchen Themen und Methoden des anti-genderistischen Diskurses, seine Akteur_Innen und gesellschaftlichen Bedingung. Dabei entwerfen sie ein sehr kohärentes Porträt dieser Politiken, das allerdings von einer weiteren Untersuchung der gesellschaftlichen Bedingungen und intersektionalen Bezüge zu anderen Ressentiments profitieren würde.

  7. UHPLC: The Greening Face of Liquid Chromatography.

    Science.gov (United States)

    Cielecka-Piontek, Judyta; Zalewski, Przemysław; Jelińska, Anna; Garbacki, Piotr

    2013-01-01

    Pharmaceutical analysis based on chromatographic separation is an important part of studies aimed at developing routine quality analysis of drugs. High-performance liquid chromatography (HPLC) is one of the main analytical techniques recommended for drug analysis. Although it meets many criteria vital for analysis, it is time-consuming and uses a relatively high amount of organic solvents compared to other analytical techniques. Recently, Ultra-high-performance liquid chromatography (UHPLC) has been frequently proposed as an alternative to HPLC, which means introducing an environment-friendly approach to drug analysis achieved by reducing the consumption of solvents. It also offers greater chromatographic resolution and higher sensitivity as well as requiring less time due to faster analysis. This review focuses on the basics of UHPLC, compares that technique with HPLC and discusses the possibilities of applying UHPLC for the analysis of different pharmaceuticals and biopharmaceuticals.

  8. Gas chromatography of β-diketonates

    International Nuclear Information System (INIS)

    Burgett, C.A.

    1976-01-01

    The history of separation of metal complexes by gas chromatography is reviewed. The greatest success has been achieved with metal halides or metal β-diketonates, particularly with chelates of the small elements, Be and Al, and the extremely inert chromium diketonates. Many problems still remain concerning the development of suitable chromatographic techniques for many chelates; however, the technique of synergic solvent extraction, mixed-ligand complex chromatography, the synthesis of new heteroatomic ligands, and the advent of new liquid phases are discussed as means to increase the number of unique techniques for separation and analysis of metals. Methods are suggested and references are cited for chromatographic analysis of elements in the following groups: alkali metals, alkaline earths, Group IIIA, IVB, VB, VIB, VIIB, VIII, IB, and IIB. Special attention is given to separation of the volatile complexes of Sc, Y, the lanthanides, the actinides, and Pb. 156 references

  9. Boron isotopic enrichment by displacement chromatography

    International Nuclear Information System (INIS)

    Mohapatra, K.K.; Bose, Arun

    2014-01-01

    10 B enriched boron is used in applications requiring high volumetric neutron absorption (absorption cross section- 3837 barn for thermal and 1 barn for 1 MeV fast neutron). It is used in fast breeder reactor (as control rod material), in neutron counter, in Boron Neutron Capture Therapy etc. Owing to very small separation factor, boron isotopic enrichment is a complex process requiring large number of separation stages. Heavy Water Board has ventured in industrial scale production of 10 B enriched boron using Exchange Distillation Process as well as Ion Displacement Chromatography Process. Ion Displacement Chromatography process is used in Boron Enrichment Plant at HWP, Manuguru. It is based on isotopic exchange between borate ions (B(OH) 4 - ) on anion exchange resin and boric acid passing through resin. The isotopic exchange takes place due to difference in zero point energy of 10 B and 11 B

  10. Instrumentation for analytical scale supercritical fluid chromatography.

    Science.gov (United States)

    Berger, Terry A

    2015-11-20

    Analytical scale supercritical fluid chromatography (SFC) is largely a sub-discipline of high performance liquid chromatography (HPLC), in that most of the hardware and software can be used for either technique. The aspects that separate the 2 techniques stem from the use of carbon dioxide (CO2) as the main component of the mobile phase in SFC. The high compressibility and low viscosity of CO2 mean that pumps, and autosamplers designed for HPLC either need to be modified or an alternate means of dealing with compressibility needs to be found. The inclusion of a back pressure regulator and a high pressure flow cell for any UV-Vis detector are also necessary. Details of the various approaches, problems and solutions are described. Characteristics, such as adiabatic vs. isothermal compressibility, thermal gradients, and refractive index issues are dealt with in detail. Copyright © 2015 Elsevier B.V. All rights reserved.

  11. Spillage detector for liquid chromatography systems

    Science.gov (United States)

    Jarvis, M. J.; Fulton, D. S. (Inventor)

    1986-01-01

    A spillage detector device for use in conjunction with fractionation of liquid chromatography systems which includes a spillage recieving enclosure beneath the fractionation area is described. A sensing device having a plurality of electrodes of alternating polarity is mounted within the spillage recieving enclosure. Detection circuitry, responsive to conductivity between electrodes, is operatively connected to the sensing device. The detection circuitry feeds into the output circuitry. The output circuit has relaying and switching circuitry directed to a solenoid, an alarm system and a pump. The solenoid is connected to the pliable conduit of the chromatography system. The alarm system comprises an audio alarm and a visual signal. A 115-volt power system interconnected with the pump, the solenoid, the sensing device, and the detection and output circuitry.

  12. Integrated sampling vs ion chromatography: Mathematical considerations

    International Nuclear Information System (INIS)

    Sundberg, L.L.

    1992-01-01

    This paper presents some general purpose considerations that can be utilized when comparisons are made between the results of integrated sampling over several hours or days, and ion chromatography where sample collection times are measured in minutes. The discussion is geared toward the measurement of soluble transition metal ions in BWR feedwater. Under steady-state conditions, the concentrations reported by both techniques should be in reasonable agreement. Transient operations effect both types of measurements. A simplistic model, applicable to both sampling techniques, is presented that demonstrates the effect of transients which occur during the acquisition of a steady-state sample. For a common set of conditions, the integrated concentration is proportional to the concentration and duration of the transient, and inversely proportional to the sample collection time. The adjustment of the collection period during a known transient allows an estimation of peak transient concentration. Though the probability of sampling a random transient with the integrated sampling technique is very high, the magnitude is severely diluted with long integration times. Transient concentrations are magnified with ion chromatography, but the probability of sampling a transient is significantly lower using normal ion chromatography operations. Various data averaging techniques are discussed for integrated sampling and IC determinations. The use of time-weighted averages appears to offer more advantages over arithmetic and geometric means for integrated sampling when the collection period is variable. For replicate steady-state ion chromatography determinations which bracket a transient sample, it may be advantageous to ignore the calculation of averages, and report the data as trending information only

  13. Use of macroporous plastics in extraction chromatography

    International Nuclear Information System (INIS)

    Braun, T.; Farac, A.B.

    1978-01-01

    Possibilities are analysed which the use of porous plastics (polyvinyl chloride, siloxane rubber, polyurethane, polystyrene and etc.) as carriers presents in extraction chromatography. Short characteristics of chemical and physical properties of macroporous carriers is given. The importance of correct chromatographic column packing is noted to obtain columns with good hydrodynamic characteristics and operational properties. Examples of using columns with macroporous carriers in radiochemistry and inorganic chemistry for element separation are given

  14. Ion exchange and liquid column chromatography

    International Nuclear Information System (INIS)

    Walton, H.F.

    1980-01-01

    Major accomplishments in liquid chromatography for the past two years and reported in journals available to December 31, 1979 are reviewed. Emphasis has been placed on principles and methodology with some representative examples of application included. Applications are illustrated in two rather lenghtly tables, one covering inorganic ions and the other covering organic compounds. 544 references, 8 books, and 15 reviews are listed in the bibliography

  15. Design of preparative-supercritical fluid chromatography.

    Science.gov (United States)

    Rajendran, Arvind

    2012-08-10

    Preparative supercritical fluid chromatography (prep-SFC) is an important separation process in the chromatographers toolbox. Owing to the unique properties of the mobile phase, which is predominantly CO(2), the behavior of SFC is markedly different from high performance liquid chromatography (HPLC). This review article focuses on the scale-up of preparative chromatography. The basics of SFC, with particular focus on highlighting the key differences between SFC and HPLC, are introduced. Then, a framework for rational design of prep-SFC is proposed. This framework is based on obtaining basic system parameters from analytical scale equipment, i.e., with very small amount of material, and performing design and optimization in silico to evaluate process performance and to identify operating conditions for scale-up. The tools required to obtain the input parameters such as adsorption isotherms are discussed and the development of the design and optimization framework is elaborated. Examples from the literature which use this approach for successful scale-up are provided. Finally the design of multi-column SFC systems is discussed. Copyright © 2012 Elsevier B.V. All rights reserved.

  16. Compound immobilization and drug-affinity chromatography.

    Science.gov (United States)

    Rix, Uwe; Gridling, Manuela; Superti-Furga, Giulio

    2012-01-01

    Bioactive small molecules act through modulating a yet unpredictable number of targets. It is therefore of critical importance to define the cellular target proteins of a compound as an entry point to understanding its mechanism of action. Often, this can be achieved in a direct fashion by chemical proteomics. As with any affinity chromatography, immobilization of the bait to a solid support is one of the earliest and most crucial steps in the process. Interfering with structural features that are important for identification of a target protein will be detrimental to binding affinity. Also, many molecules are sensitive to heat or to certain chemicals, such as acid or base, and might be destroyed during the process of immobilization, which therefore needs to be not only efficient, but also mild. The subsequent affinity chromatography step needs to preserve molecular and conformational integrity of both bait compound and proteins in order to result in the desired specific enrichment while ensuring a high level of compatibility with downstream analysis by mass spectrometry. Thus, the right choice of detergent, buffer, and protease inhibitors is also essential. This chapter describes a widely applicable procedure for the immobilization of small molecule drugs and for drug-affinity chromatography with subsequent protein identification by mass spectrometry.

  17. Present evaluation of the use of rape oil/RME compared to diesel fuel. Appendix 1: Resources and emission balances: rape oil and RME compared to diesel fuel. Appendix 2: Economic evaluation of the use of rape oil and RME compared to diesel fuel; Aktuelle Bewertung des Einsatzes von Rapsoel/RME im Vergleich zu Dieselkraftstoff. Anhang 1: Ressourcen- und Emissionsbilanzen: Rapsoel und RME im Vergleich zu Dieselkraftstoff. Anhang 2: Oekonomische Bewertung des Einsatzes von Rapsoel und RME gegenueber Dieselkraftstoff

    Energy Technology Data Exchange (ETDEWEB)

    Kraus, K.; Niklas, G.; Tappe, M.; Reinhardt, G.A. [ifeu-Institut fuer Energie- und Umweltforschung GmbH, Heidelberg (Germany); Folkers, C. [Bochum Univ. (Germany). Lehrstuhl fuer Finanzwissenschaft

    1999-12-01

    New recognitions in the sectors of research, development in the processing industry, motor vehicle technology, ecological balance methodics, the high interest in environmental policy and some other points of criticism have motivated the Umweltbundesamt to update the corresponding parts of the '1993 eco-balance rape oil'. Balancing of the particularly critically discussed sectors of resource consumption and airborne environmental impacts over the entire life cycle of rape oil based fuels as compared to diesel fuel was taken care of by the ifeu Institute. That balances were only made up for these two sectors is due to the fact that it is here where the particular environmental advantages of vegetable fuel oils are supposedly brought to bear. Moreover, on the basis of the report 'renewable energy sources - fundamentals, methods, ecological balancing' published recently and sponsored by the Deutsche Bundesstiftung Umwelt, topical essential factors such as the redefinition of the reference system (short-rotation cultivation instead of permanent fallowness) could be taken into account by the expertise. A particular benefit attributed to rape oil and RME is the conservation of fossil energy sources and associated abatement of the greenhouse effect (especially carbon dioxide). Cutting down greenhouse gas emissions is one of the predominant aims of national and international environmental policy with a view to preventing a dangerous man-made disturbance of climate. (orig.) [German] Neue Erkenntnisse im Bereich der Forschung, der Entwicklungen der verarbeitenden Industrie, der Fahrzeugtechnik, der Oekobilanzmethodik, das hohe umweltpolitische Interesse sowie die zuvor genannten Kritikpunkte haben das Umweltbundesamt veranlasst, die entsprechenden Teile der 'Oekobilanz Rapsoel' von 1993 auf den neuesten Stand zu bringen. Das ifeu-Institut hat die besonders kritisch diskutierten Bereiche des Ressourcenverbrauchs und der luftgetragenen

  18. Technological options in regard to reducing harmful environmental effects of road traffic. Fuel cell technology compared with combustion engines; Technische Optionen zur Verminderung der Verkehrsbelastungen. Brennstoffzellenfahrzeuge im Vergleich zu Fahrzeugen mit Verbrennungsmotoren

    Energy Technology Data Exchange (ETDEWEB)

    Kolke, R. [comp.

    1999-05-01

    The German Umweltbundesamt (federal environmental agency) commissioned a cost-benefit analysis to be made, testing the practical performance of fuel cell-driven automobiles, for state-of-the-art assessment of their benefits and drawbacks as compared to other available automotive fuel systems. Results available so far of calculations based on the cost-benefit analysis show that the targets involved, such as cutback of emissions and conservation of resources, can be realised within the foreseeable future at much lesser expense through the use of fuel consumption-optimized Otto engines offering lowest emission levels (of the type ULEV, ultra-low emission vehicle, or EURO 4 standard), as compared to the relevant contributions to emission reduction and other targets offered by the fuel cell-driven automobiles.(orig./CB) [Deutsch] Das Umweltbundesamt hat die Brennstoffzellentechnik im Verkehr einer Kosten-Nutzen-Betrachtung unterzogen, um zu ermitteln, welche Vor- und Nachteile sie im Vergleich zu anderen Antriebskonzepten im Verkehr nach derzeitigem Wissensstand hat. Nach den bisherigen Berechnungen am Beispiel einer Kosten-Nutzen-Analyse zeichnet sich ab, dass Emissionsminderungen und Ressourcenschutz fuer die absehbare Zeit wesentlich kostenguenstiger durch verbrauchsoptimierte Fahrzeuge mit Ottomotor mit niedrigsten Abgasemissionen (ULEV-`Ultra Low Emission Vehicle` oder EURO 4-Standard) realisiert werden koennen als durch Fahrzeuge mit Brennstoffzellenantrieb. (orig.)

  19. „Ich glaube, wir können es uns gar nicht leisten, nichts von Religion zu verstehen.“ Grundkompetenzen für Lehrerinnen und Lehrer im Umgang mit Religion

    Directory of Open Access Journals (Sweden)

    Helga Kohler-Spiegel

    2016-06-01

    Full Text Available Das Recht von Kindern, sich mit Religion auseinanderzusetzen, ist in den Kinderrechten der UN verankert, Friedrich Schweitzer spricht von den „großen Fragen“. Diesen Fragen Raum zu geben beinhaltet ein Begleiten der Kinder, unabhängig ihrer formalen religiösen Zugehörigkeiten. Da sich Religiöses in der Schule auf sehr vielfältige Art zeigt, bedarf es der kurzen Klärung, was darunter verstanden wird, bevor beschrieben wird, welche Grundkompetenzen Lehrpersonen brauchen, um Kinder in der Vielfalt dieser Erfahrungen und Fragen zu begleiten. „I believe we cannot afford to not understand religion.“ Basic competences for teachers in handling religion Children‘s entitlement to deal with religion is constituted in the Human Rights for Children of the UN, Friedrich Schweitzer refers to it as „The big questions“. To create space for these questions implies to shepherd the children, regardless of their formal religious affiliation. Driven by the diversity of religiousness in school, some brief clarification of this topic is required, before characterizing which basic expertise educational personnel need to have in order to guide children through the variety of these experiences and questions.

  20. Dust precipitation nuisance in the Rhine lignite basin. Situation analysis, legal assessment and the use of compost recycling for emission-protection purposes; Die Staubniederschlagsbelastungen im Rheinischen Braunkohlenrevier. Situationsanalyse, rechtliche Bewertung und Verwertung von Kompost zu Immissionsschutzzwecken

    Energy Technology Data Exchange (ETDEWEB)

    Asenbaum, P. [Bergamt Dueren (Germany)

    2004-03-01

    The paper describes the problem of dust precipitation in the Rhine lignite basin and presents a legal, scientific and technical assessment of how composting can be used for emission protection purposes, along with supporting measures such as the replanting of opencast mining sites. Also examines the benefits, and possible risks, associated with waste recycling projects of this kind. The results of the investigations show that compost recycling for emission-protection purposes can be used to support the emission-protection measures previously deployed at opencast mines in the Rhine lignite basin, including the grassing-over of former mine sites. Such measures are now considered to be technically feasible, environmentally compatible and lawful, and indeed are considered necessary in some circumstances. (orig.) [German] Die Arbeit beschreibt die Staubniederschlagsbelastungen im Rheinischen Braunkohlenrevier und bewertet in diesem Zusammenhang den Einsatz von Kompost zu Immissionsschutzzwecken einschliesslich einer unterstuetzenden Begruenung in den Tagebauen in rechtlicher sowie naturwissenschaftlich-technischer Hinsicht. Darueber hinaus wird die Nuetzlichkeit und Schadlosigkeit dieser Abfallverwertungsmassnahmen beurteilt. Nach den Ergebnissen dieser Arbeit ist eine Kompostverwertung zu Immissionsschutzzwecken zur Ergaenzung des bisherigen Immissionsschutzsystems in den Tagebauen des Rheinischen Braunkohlenreviers einschliesslich einer unterstuetzenden Begruenung technisch moeglich, umweltvertraeglich und rechtlich zulaessig sowie unter bestimmten Voraussetzungen erforderlich. (orig.)

  1. E-Portfolios, ‹eine Möglichkeit, viel für sich selbst zu lernen›. Der Einsatz von E-Portfolios als Reflexionsinstrument am Beispiel der Viadrina PeerTutoring-Ausbildung

    Directory of Open Access Journals (Sweden)

    Stefanie Vogler-Lipp

    2017-03-01

    Full Text Available Das Reflektieren und Verstehen des eigenen Lernprozesses gehört zu den studienrelevanten Fertigkeiten, die erlernt und während eines erfolgreichen Studiums vertieft werden sollten. Seit 2012 wird im Rahmen der Viadrina PeerTutoring-Ausbildung an der Europa-Universität Viadrina auf den Ausbau und die Weiterentwicklung der Reflexionskompetenzen sehr viel Wert gelegt. Im Folgenden werden die theoretischen Grundlagen zusammengefasst und erläutert, wie gutes Reflektieren angeleitet wird. Die Studierenden reflektieren ihre Lernprozesse in E-Portfolios. Um das komplexe Zusammenspiel einer guten Anleitung zum Reflektieren, den tatsächlichen Ergebnissen in den E-Portfolios und den Eindrücken der Mitarbeiter/innen am Zentrum für Schlüsselkompetenzen und Forschendes Lernen nachvollziehen zu können, werden am Ende einzelne Auszüge aus den E-Portfolios vorgestellt und für den Einsatz von E-Portfolio als Reflexionsinstrument plädiert.

  2. Christine Löw: Frauen aus der Dritten Welt und Erkenntniskritik? Die postkolonialen Untersuchungen von Gayatri C. Spivak zu Globalisierung und Theorieproduktion. Sulzbach im Taunus: Ulrike Helmer Verlag 2009.

    Directory of Open Access Journals (Sweden)

    Heike Kahlert

    2011-07-01

    Full Text Available Christine Löw setzt sich in ihrer politikwissenschaftlichen Dissertation mit dem postkolonial-feministischen Denken Gayatri C. Spivaks auseinander und zielt in ihren sorgfältigen, überwiegend affirmativen Ausführungen darauf, Spivaks Überlegungen für die Politikwissenschaft nutzbar zu machen. Hierzu diskutiert sie deren theoretische Verortung in kritischen Ansätzen von Marxismus, Feminismus und Dekonstruktion sowie ihre Reflexionen zur epistemischen Gewalt westlichen Denkens gegenüber dem Süden und illustriert ihre Thesen zur Subalternität an vier ausgewählten politischen Themenfeldern. Zentrale Verdienste des Buches liegen darin, Spivaks Arbeiten in den deutschsprachigen sozialwissenschaftlichen Kontext einzuschreiben und weiterführende Forschungsfragen zu Globalisierung und Erkenntnisproduktion aufzuwerfen.Christine Löw’s dissertation (in the field of Political Science examines Gayatri C. Spivak’s postcolonial-feminist thinking. In her careful, mainly affirmative explanations she aims at utilizing Spivak’s considerations for Political Science. To this end, she discusses their theoretical position within critical approaches of Marxism, feminism, and deconstruction as well as her reflections on epistemic violence of western thinking against the south while also illustrating her theses on subalternity with four selected political topics. Situating Spivak’s works within the context of German-speaking social science and posing additional research questions on globalization and production of knowledge are the main achievements of this book.

  3. Präferenzen, Wohlergehen und Rationalität – Zu den begrifflichen Grundlagen des libertären Paternalismus und ihren Konsequenzen für seine Legitimierbarkeit

    Directory of Open Access Journals (Sweden)

    Klonschinski Andrea

    2016-07-01

    Full Text Available Der libertäre Paternalismus (LP genießt in Politik, Wissenschaft und Öffentlichkeit eine große Popularität, die er insbesondere zwei Merkmalen verdankt: Erstens stützt sich der LP auf verhaltensökonomische Ergebnisse, die zeigen, dass individuelle Entscheidungen oft nicht der neoklassischen Rationalitätskonzeption entsprechen, sodass Individuen durch sogenanntes Nudging zu besseren, ihren wahren Präferenzen entsprechenden Entscheidungen verholfen werden könne. Zweites ist damit der Anspruch verbunden, das Wohlergehen der Individuen, wie sie selbst es verstehen, zu erhöhen. Dieser Beitrag zeigt anhand einer dogmengeschichtlichen Analyse der zentralen, dem LP zugrunde liegenden Begriffe Präferenz, Nutzen, Rationalität und Wohlergehen, dass der LP diesen Anspruch nicht einlösen kann. Eine detaillierte begriffliche Analyse ist deshalb geboten, weil diese Konzepte in der Ökonomik immer wieder für Missverständnisse sorgen. Eine theoriegeschichtlich informierte Untersuchung wiederum ist notwendig, da diese Konfusion insbesondere auf der Tatsache beruht, dass der Gehalt dieser Begriffe sich im Laufe der Zeit gravierend verändert hat und sich die verschiedenen Bedeutungen heute z. T. wechselseitig überlagern.

  4. Ein (erneuter Versuch, modernen Ungleichheitsstrukturen auf die Spur zu kommen An(other Attempt at Getting at the Core of Modern Structures of Inequality

    Directory of Open Access Journals (Sweden)

    Irene Dölling

    2001-11-01

    Full Text Available Die Verschränkung zweier Ungleichheitsstrukturen – der ‚klassischen‘ Sozialstruktur und der Struktur der Geschlechterverhältnisse – ist für die Autorin eine Ursache dafür, dass seit Einführung der Marktwirtschaft ostdeutsche Frauen massiv vom Arbeitsmarkt verdrängt werden. Aufbauend auf Grundannahmen über die Entstehung moderner Ungleichheitsstrukturen vergleicht Schäfgen die Entwicklung von Sozialstruktur und Geschlechterverhältnissen der BRD und der DDR, die sie beide als moderne Gesellschaften versteht – leider nicht gesellschafts- bzw. modernetheoretisch begründet. Dies hat Konsequenzen: Zwar bestätigt das empirische Material das Vorhandensein ‚klassischer‘ wie auch qua Geschlecht erzeugter Ungleichheiten in beiden deutschen Staaten, aber Schäfgens Vergleich liefert leider keine Differenzierung zwischen den Ausprägungen der Moderne in BRD und DDR. Um die ‚doppelte Ungleichheit‘ in die Analyse einbeziehen zu können, entwickelt Schäfgen das ‚Modell der doppelten Vertikalität‘. Im Kern dieses Modells werden die durch ‚Geschlecht‘ produzierten Ungleichheiten in deren vertikaler Dimension verortet. Fraglich ist, ob Schäfgens Modell theoretischen und methodischen Gewinn bringt: Kann die Substantialisierung von Konzepten wie ‚Klasse‘ oder ‚Geschlecht‘ wirklich der prozessualen Erzeugung und Reproduktion sozialer Ungleichheit auf die Spur kommen?Schäfgen views the interlocking of two systems of inequality-that of ‘traditional’ social inequality and that of gender inequality-as one reason why East German women have been driven out of the labour market since the introduction of a free market economy. Starting from core assumptions about the development of modern structures of inequality, Schäfgen compares the development of social structures and gender relations in the FRG and GDR. Schäfgen merely presumes the two German states to be modern states; unfortunately, she does not explain why she

  5. Analysis of pollutants in air and water using gas chromatography and headspace gas chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Stenner, H.

    1980-01-01

    The combination 'personal sampling' with headspace gas chromatography to determine traces of formaldehyde, phenol and benzene in air is investigated in this work, with the aim of developing maximum workplace concentration values (MWL values). Further possible applications of gas chromatography in trace analysis in the environmentally protected area. The analysis of chromium in waste waters (Cr III as acetyl acetonate complex) is investigated as further possible application, whereby optimum conditions are obtained. A modified flame ionization detector was used to increase the detection sensitivity.

  6. Thin-layer chromatography - liquid chromatography, an ideal supplement demonstrated by the separation of lanthanoids

    International Nuclear Information System (INIS)

    Specker, H.; Hufnagel, A.

    1984-01-01

    All lanthanoids have been separated by thin-layer chromatography (TLC) with short retention times by using a mixture of ether/tetrahydrofurane (THF)/bis-(2-ethylhexyl)-phosphate (HDEHP)/nitric acid. The eluent was empirically tested by synergistic effects. The results have been transferred to high-performance liquid chromatography /HPLC). It was possible to use the same eluent in TLC and HPLC both for the analytical separation of all lanthanoids and for the separation of fission products. The experimental experience gained in HPLC could be applied to the pre-concentration of isotopes in TLC. Both methods excellently supplemented each other in the separation of lanthanoids. (orig.) [de

  7. Tutorial: simulating chromatography with Microsoft Excel Macros.

    Science.gov (United States)

    Kadjo, Akinde; Dasgupta, Purnendu K

    2013-04-22

    Chromatography is one of the cornerstones of modern analytical chemistry; developing an instinctive feeling for how chromatography works will be invaluable to future generation of chromatographers. Specialized software programs exist that handle and manipulate chromatographic data; there are also some that simulate chromatograms. However, the algorithm details of such software are not transparent to a beginner. In contrast, how spreadsheet tools like Microsoft Excel™ work is well understood and the software is nearly universally available. We show that the simple repetition of an equilibration process at each plate (a spreadsheet row) followed by discrete movement of the mobile phase down by a row, easily automated by a subroutine (a "Macro" in Excel), readily simulates chromatography. The process is readily understood by a novice. Not only does this permit simulation of isocratic and simple single step gradient elution, linear or multistep gradients are also easily simulated. The versatility of a transparent and easily understandable computational platform further enables the simulation of complex but commonly encountered chromatographic scenarios such as the effects of nonlinear isotherms, active sites, column overloading, on-column analyte degradation, etc. These are not as easily simulated by available software. Views of the separation as it develops on the column and as it is seen by an end-column detector are both available in real time. Excel 2010™ also permits a 16-level (4-bit) color gradation of numerical values in a column/row; this permits visualization of a band migrating down the column, much as Tswett may have originally observed, but in a numerical domain. All parameters of relevance (partition constants, elution conditions, etc.) are readily changed so their effects can be examined. Illustrative Excel spreadsheets are given in the Supporting Information; these are easily modified by the user or the user can write his/her own routine. Copyright

  8. Phosphopeptide enrichment by immobilized metal affinity chromatography

    DEFF Research Database (Denmark)

    Thingholm, Tine E.; Larsen, Martin R.

    2016-01-01

    Immobilized metal affinity chromatography (IMAC) has been the method of choice for phosphopeptide enrichment prior to mass spectrometric analysis for many years and it is still used extensively in many laboratories. Using the affinity of negatively charged phosphate groups towards positively...... charged metal ions such as Fe3+, Ga3+, Al3+, Zr4+, and Ti4+ has made it possible to enrich phosphorylated peptides from peptide samples. However, the selectivity of most of the metal ions is limited, when working with highly complex samples, e.g., whole-cell extracts, resulting in contamination from...

  9. Undergraduate physics laboratory: Electrophoresis in chromatography paper

    Science.gov (United States)

    Hyde, Alexander; Batishchev, Oleg

    2015-12-01

    An experiment studying the physical principles of electrophoresis in liquids was developed for an undergraduate laboratory. We have improved upon the standard agarose gel electrophoresis experimental regime with a straightforward and cost-effective procedure, in which drops of widely available black food coloring were separated by electric field into their dye components on strips of chromatography paper soaked in a baking soda/water solution. Terminal velocities of seven student-safe dyes were measured as a function of the electric potential applied along the strips. The molecular mobility was introduced and calculated by analyzing data for a single dye. Sources of systematic and random errors were investigated.

  10. Gas chromatography of alkylphosphonic and dialkylphosphinic acids

    International Nuclear Information System (INIS)

    Gasco, L.; Barrera, R.; Ramirez, A.; Martin Munoz, N.

    1978-01-01

    After carrying out an optimization study on the separation conditions for the TMS-derivatives, of the hexyl-, cyclohexyl-, heptyl-, and octyl-phosphonic acids; dihexyl-, dicyclohexyl-, heptyl-, and octyl-phosphinic acids, and dioctylphosphine oxide, their retention indices (I) at two temperatures and on the 0V-1 and 0V-17 stationary phase were determined. Correlations between I and molecular structure were established. Calibration factors of these compounds in the flame ionization detector were studied, and the results analized taking into account the variables afecting the quantitative results. These results were unbiased but they had a lower precission than usually achieveble in gas chromatography. (author)

  11. Quantitative analysis of deuterium by gas chromatography

    International Nuclear Information System (INIS)

    Isomura, Shohei; Kaetsu, Hayato

    1981-01-01

    An analytical method for the determination of deuterium concentration in water and hydrogen gas by gas chromatography is described. HD and D 2 in a hydrogen gas sample were separated from H 2 by a column packed with Molecular Sieve 13X, using extra pure hydrogen gas as carrier. A thermal conductivity detector was used. Concentrations of deuterium were determined by comparison with standard samples. The error inherent to the present method was less a 1% on the basis of the calibration curves obtained with the standard samples. The average time required for the analysis was about 3 minutes. (author)

  12. HDT mixtures treatment strategies by gas chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Laquerbe, C.; Contreras, S.; Demoment, J. [Commissariat a l' Energie Atomique - CEA/Valduc, F-21121 Is sur Tille (France)

    2008-07-15

    Gas phase chromatographic processes are of interest for the separation of hydrogen isotopes from an HDT mixture. For a certain quantity, they are very competitive and present several benefits. Nevertheless no active packing material allows to have simultaneously good enrichment performances for tritium production and high decontamination capabilities for HD gases. The influence of the packing material is first described in this article. Then two specific processes (TCAP and Reverse Chromatography), each well adapted to perform one target, are presented. Finally, the problematic to propose an optimized treatment scheme associating these two processes is formulated. (authors)

  13. HDT mixtures treatment strategies by gas chromatography

    International Nuclear Information System (INIS)

    Laquerbe, C.; Contreras, S.; Demoment, J.

    2008-01-01

    Gas phase chromatographic processes are of interest for the separation of hydrogen isotopes from an HDT mixture. For a certain quantity, they are very competitive and present several benefits. Nevertheless no active packing material allows to have simultaneously good enrichment performances for tritium production and high decontamination capabilities for HD gases. The influence of the packing material is first described in this article. Then two specific processes (TCAP and Reverse Chromatography), each well adapted to perform one target, are presented. Finally, the problematic to propose an optimized treatment scheme associating these two processes is formulated. (authors)

  14. Course on Advanced Analytical Chemistry and Chromatography

    DEFF Research Database (Denmark)

    Andersen, Jens Enevold Thaulov; Fristrup, Peter; Nielsen, Kristian Fog

    2011-01-01

    Methods of analytical chemistry constitute an integral part of decision making in chemical research, and students must master a high degree of knowledge, in order to perform reliable analysis. At DTU departments of chemistry it was thus decided to develop a course that was attractive to master...... students of different direction of studies, to Ph.D. students and to professionals that need an update of their current state of skills and knowledge. A course of 10 ECTS points was devised with the purpose of introducing students to analytical chemistry and chromatography with the aim of including theory...

  15. Use of chelating agents as immovable phase in extraction chromatography

    International Nuclear Information System (INIS)

    Sebesta, F.

    1978-01-01

    Extraction chromatography using chelating agents is reviewed. The theory of element extraction by chelating agents and factors influencing this process (pH, extracting agent concentration in organic phase, masking agent concentration in aqueous phase) are briefly considered. The effect of kinetic factors on the extraction chromatography process is discussed. Ways of preparing columns are emphasized. Examples of using chelating reagents in various extraction chromatography systems are given. β-Diketones, oximes, hydroxamic acids, dithizon, diethyl dithiocarbamic acid are chosen as chelating agents

  16. [High-performance liquid-liquid chromatography in beverage analysis].

    Science.gov (United States)

    Bricout, J; Koziet, Y; de Carpentrie, B

    1978-01-01

    Liquid liquid chromatography was performed with columns packed with stationary phases chemically bonded to silica microparticules. These columns show a high efficiency and are used very easily. Flavouring compounds like aromatic aldehydes which have a low volatility were analyzed in brandy using a polar phase alkylnitrile. Sapid substances like amarogentin in Gentiana lutea or glyryrrhizin in Glycyrrhiza glabra were determined by reversed phase chromatography. Finally ionizable substances like synthetic dyes can be analyzed by paired ion chromatography witha non polar stationary phase.

  17. Optimizing separations in online comprehensive two‐dimensional liquid chromatography

    Science.gov (United States)

    Gargano, Andrea F.G.; Schoenmakers, Peter J.

    2017-01-01

    Abstract Online comprehensive two‐dimensional liquid chromatography has become an attractive option for the analysis of complex nonvolatile samples found in various fields (e.g. environmental studies, food, life, and polymer sciences). Two‐dimensional liquid chromatography complements the highly popular hyphenated systems that combine liquid chromatography with mass spectrometry. Two‐dimensional liquid chromatography is also applied to the analysis of samples that are not compatible with mass spectrometry (e.g. high‐molecular‐weight polymers), providing important information on the distribution of the sample components along chemical dimensions (molecular weight, charge, lipophilicity, stereochemistry, etc.). Also, in comparison with conventional one‐dimensional liquid chromatography, two‐dimensional liquid chromatography provides a greater separation power (peak capacity). Because of the additional selectivity and higher peak capacity, the combination of two‐dimensional liquid chromatography with mass spectrometry allows for simpler mixtures of compounds to be introduced in the ion source at any given time, improving quantitative analysis by reducing matrix effects. In this review, we summarize the rationale and principles of two‐dimensional liquid chromatography experiments, describe advantages and disadvantages of combining different selectivities and discuss strategies to improve the quality of two‐dimensional liquid chromatography separations. PMID:29027363

  18. Optimizing separations in online comprehensive two-dimensional liquid chromatography.

    Science.gov (United States)

    Pirok, Bob W J; Gargano, Andrea F G; Schoenmakers, Peter J

    2018-01-01

    Online comprehensive two-dimensional liquid chromatography has become an attractive option for the analysis of complex nonvolatile samples found in various fields (e.g. environmental studies, food, life, and polymer sciences). Two-dimensional liquid chromatography complements the highly popular hyphenated systems that combine liquid chromatography with mass spectrometry. Two-dimensional liquid chromatography is also applied to the analysis of samples that are not compatible with mass spectrometry (e.g. high-molecular-weight polymers), providing important information on the distribution of the sample components along chemical dimensions (molecular weight, charge, lipophilicity, stereochemistry, etc.). Also, in comparison with conventional one-dimensional liquid chromatography, two-dimensional liquid chromatography provides a greater separation power (peak capacity). Because of the additional selectivity and higher peak capacity, the combination of two-dimensional liquid chromatography with mass spectrometry allows for simpler mixtures of compounds to be introduced in the ion source at any given time, improving quantitative analysis by reducing matrix effects. In this review, we summarize the rationale and principles of two-dimensional liquid chromatography experiments, describe advantages and disadvantages of combining different selectivities and discuss strategies to improve the quality of two-dimensional liquid chromatography separations. © 2017 The Authors. Journal of Separation Science published by WILEY-VCH Verlag GmbH & Co. KGaA.

  19. Liquid chromatography - mass spectrometry analysis of pharmaceuticals

    International Nuclear Information System (INIS)

    Macasek, F.

    2003-01-01

    The drugs represent mostly non-volatile and thermally labile solutes, often available only in small amounts like it is in case of radiopharmaceuticals. Therefor, the favourable separation techniques for such compounds are HPLC, capillary electrophoresis and also TLC 1. Liquid chromatography with mass spectrometric detector (LC/MS) is especially powerful for their microanalysis. Mass spectrometry separating the ions in high vacuum was presumably used as detector for gas chromatography effluent but the on-line coupling with liquid eluant flow 0.1-1 mL/min is far more challenging. New types of ion sources were constructed for simultaneous removal of solvent and ionisation of solutes at atmospheric pressure (API). At present, a relatively wide choice of successfully designed commercial equipment is available either for small organic molecules and larger biomolecules (Perkin-Elmer, Agilent, Jeol, Bruker Daltonics, ThermoQuest, Shimadzu). The features of the LC/MS systems are presented. LC/MS as a new quality control tool for [F-18]fluorodeoxyglucose (FDG) radiopharmaceutical, which has became the most spread radiopharmaceutical for positron emission tomography (PET), was proposed. Other applications of the LC/MS are reviewed. (author)

  20. Alternatives to Chromatography in Plant Breeding

    Directory of Open Access Journals (Sweden)

    Keusgen, Michael

    2016-07-01

    Full Text Available Wild plants were taken into cultivation because of special features. Usually, medicinal plants or spices show distinct secondary metabolites combined with a specific pattern of these compounds. Typically, chromatographic methods like gas chromatography (GC or high performance liquid chromatography (HPLC were applied as standard methods for a meaningful analysis of secondary metabolites. However, these methods are labor and time intensive. In the breeding process, usually numerous single plants have to be analyzed and therefore, high throughput methods are required. In this article, some examples for alternative strategies are given. Besides spectroscopic methods like near infrared (NIR, also biosensoric approaches should be considered. For instance, several enzymes can oxidize or hydrolyze secondary metabolites in dependence of their functional groups. Polyphenols can be determined by laccases. Polyphenols like catechins and flavonoids contribute to the bioactivity of many medicinal plants. Also cysteine sulfoxides, which are typical for Allium species like garlic and onions, can be enzymatically determined with high specificity. Finally, toxic cyanogenic glycosides can be quantified by the enzyme cyanidase.

  1. The weight of flash chromatography: A tool to predict its mass intensity from thin-layer chromatography

    Directory of Open Access Journals (Sweden)

    Freddy Pessel

    2016-11-01

    Full Text Available Purification by flash chromatography strongly impacts the greenness of a process. Unfortunately, due to the lack of the relevant literature data, very often this impact cannot be assessed thus preventing the comparison of the environmental factors affecting the syntheses. We developed a simple mathematical approach to evaluate the minimum mass intensity of flash chromatography from the retention factor values determined by thin-layer chromatography.

  2. Radial Chromatography for the Separation of Nitroaniline Isomers

    Science.gov (United States)

    Miller, Robert B.; Case, William S.

    2011-01-01

    Separation techniques are usually presented in the undergraduate organic laboratory to teach students how to purify and isolate compounds. Often the concept of liquid chromatography is introduced by having students create "silica gel columns" to separate components of a reaction mixture. Although useful, column chromatography can be a laborious…

  3. Using Ion Exchange Chromatography to Separate and Quantify Complex Ions

    Science.gov (United States)

    Johnson, Brian J.

    2014-01-01

    Ion exchange chromatography is an important technique in the separation of charged species, particularly in biological, inorganic, and environmental samples. In this experiment, students are supplied with a mixture of two substitution-inert complex ions. They separate the complexes by ion exchange chromatography using a "flash"…

  4. Pseudo-affinity chromatography of rumen microbial cellulase on ...

    African Journals Online (AJOL)

    Pseudo-affinity chromatography of rumen microbial cellulase on Sepharose- Cibacron Blue F3GA. ... African Journal of Biotechnology ... Pseudo affinity adsorption of bioproducts on Sepharose-cibacron blue F3-GA was subjected to rumen microbial enzyme evaluation through batch binding and column chromatography of ...

  5. A retrospective data acquisition and evaluation on the characterization and prognosis for 776 patients with differentiated thyroid carcinomas in the Klinik und Poliklinik fuer Nuklearmedizin der Universitaet zu Koeln in the period from 1990 to 2005

    International Nuclear Information System (INIS)

    Meixner, Markus

    2011-01-01

    The author describes the retrospective data acquisition and evaluation on the characterization and prognosis for 776 patients (average age 40:1 years) with differentiated thyroid carcinomas in the Klinik und Poliklinik fuer Nuklearmedizin der Universitaet zu Koeln in the period from 1990 to 2005. The methodology includes patient-relevant data, examination data, imaging techniques and biochemical tests. Clinically important differences were found for papillary and follicular thyroid carcinoma. Further differences concern the localization of metastases. The author discusses the prognostic significance of the primary tumor radius in comparison with the so-called TNM classification for papillary and follicular thyroid carcinomas. It is concluded that the new version of the TNM classification offers only slight advantages with respect to the former version. It was also shown that the primary tumor size is adequate for prognostic estimations.

  6. Gel chromatography of sup(99m)Tc-labelled compounds

    International Nuclear Information System (INIS)

    Vilcek, S.; Machan, V.; Kalincak, M.

    1976-01-01

    The present state of gel chromatography of sup(99m)Tc-labelled compounds is reviewed. Examples are given of gel chromatography for preparing labelled compounds and for quality control analysis and the development of new types of sup(99m)Tc-labelled compounds. The factors which influence the gel chromatography of these compounds are discussed, i.e., the nature of the elution agent, the duration of the contact of the gel and the preparation the gel type, the nature of the labelled compound. The GCS method (gel chromatography scanning) is briefly described. The advantages of gel chromatography as compared with other chromatographic techniques for sup(99m)Tc-labelled compounds are summarized. (author)

  7. Counter-current motion in counter-current chromatography.

    Science.gov (United States)

    Ito, Yoichiro

    2014-12-12

    After the CCC2012 meeting, I have received an e-mail regarding the terminology of "Countercurrent Chromatography". It stated that the term "Countercurrent" is a misnomer, because its stationary phase is motionless in the column and that the method should be renamed as liquid-liquid separations or centrifugal separations. However, it was found that these names are already used for various other techniques as found via Google search. The term "Countercurrent Chromatography" was originally made after two preparative methods of Countercurrent distribution and liquid Chromatography, both having no countercurrent motion in the column. However, it is surprising to find that this F1 hybrid method "Countercurrent Chromatography" can clearly exhibit countercurrent motion within the separation column in both hydrodynamic and hydrostatic equilibrium systems. This justifies that "Countercurrent Chromatography" is a proper term for this chromatographic method. Published by Elsevier B.V.

  8. Phytochemical analysis of ethanolic extract of Dichrostachys Cinerea W and Arn leaves by a thin layer chromatography, high performance thin layer chromatography and column chromatography

    OpenAIRE

    M Vijayalakshmi; K Periyanayagam; K Kavitha; K Akilandeshwari

    2013-01-01

    Background: The leaves of Dichrostachys cinerea are used as laxative, diuretic, painkiller. It is also used in the treatment of gonorrhoea, boils, oedema, gout, veneral diseases and nasopharyngeal affections, etc. Materials and Methods: The Phytochemical investigation of ethanolic extract of D. cinerea leaves were performed by standard chemical tests, thin layer chromatography (TLC) by using various solvent systems, and by high performance liquid chromatography (HPTLC). Two compounds were...

  9. Simultaneous analysis for water- and fat-soluble vitamins by a novel single chromatography technique unifying supercritical fluid chromatography and liquid chromatography.

    Science.gov (United States)

    Taguchi, Kaori; Fukusaki, Eiichiro; Bamba, Takeshi

    2014-10-03

    Chromatography techniques usually use a single state in the mobile phase, such as liquid, gas, or supercritical fluid. Chromatographers manage one of these techniques for their purpose but are sometimes required to use multiple methods, or even worse, multiple techniques when the target compounds have a wide range of chemical properties. To overcome this challenge, we developed a single method covering a diverse compound range by means of a "unified" chromatography which completely bridges supercritical fluid chromatography and liquid chromatography. In our method, the phase state was continuously changed in the following order; supercritical, subcritical and liquid. Moreover, the gradient of the mobile phase starting at almost 100% CO2 was replaced with 100% methanol at the end completely. As a result, this approach achieved further extension of the polarity range of the mobile phase in a single run, and successfully enabled the simultaneous analysis of fat- and water-soluble vitamins with a wide logP range of -2.11 to 10.12. Furthermore, the 17 vitamins were exceptionally separated in 4min. Our results indicated that the use of dense CO2 and the replacement of CO2 by methanol are practical approaches in unified chromatography covering diverse compounds. Additionally, this is a first report to apply the novel approach to unified chromatography, and can open another door for diverse compound analysis in a single chromatographic technique with single injection, single column and single system. Copyright © 2014. Published by Elsevier B.V.

  10. Peak broadening in paper chromatography and related techniques : III. Peak broadening in thin-layer chromatography on cellulose powder

    NARCIS (Netherlands)

    Ligny, C.L. de; Remijnse, A.G.

    1968-01-01

    The mechanism of peak broadening in thin-layer chromatography on cellulose powder was investigated by comparing the peak widths obtained in chromatography with those caused only by diffusion in the cellulose powder, for a set of amino acids of widely differing RF values and six kinds of cellulose

  11. Ausrüstung von Starkstromanlagen mit elektronischen Betriebsmitteln / Achtung: Abschnitte 5.3.2.2.2 (einschließlich Kurve 2 in Bild 2) und 7.3.1.2 gelten erst ab Mai 1990. Bestehende Anlagen und Betriebsmittel einschließlich Ersatzlieferungen brauchen nicht den Anforderungen dieser neuen Norm angepaßt zu werden.

    CERN Document Server

    Deutsches Institut für Normung. Berlin

    1988-01-01

    Ausrüstung von Starkstromanlagen mit elektronischen Betriebsmitteln / Achtung: Abschnitte 5.3.2.2.2 (einschließlich Kurve 2 in Bild 2) und 7.3.1.2 gelten erst ab Mai 1990. Bestehende Anlagen und Betriebsmittel einschließlich Ersatzlieferungen brauchen nicht den Anforderungen dieser neuen Norm angepaßt zu werden.

  12. Prüfungen zur Beurteilung der Brandgefahr; Beispiele für Verfahren zur Beurteilung einer Brandgefahr und für die Auslegung von Ergebnissen; 3.1: Kennwerte der Verbrennung und Übersicht über Prüfverfahren zu ihrer Ermittlung; Identisch mit IEC 60695-3-1, Ausgabe 1982

    CERN Document Server

    Deutsches Institut für Normung. Berlin

    1986-01-01

    Prüfungen zur Beurteilung der Brandgefahr; Beispiele für Verfahren zur Beurteilung einer Brandgefahr und für die Auslegung von Ergebnissen; 3.1: Kennwerte der Verbrennung und Übersicht über Prüfverfahren zu ihrer Ermittlung; Identisch mit IEC 60695-3-1, Ausgabe 1982

  13. Alexandru Popa, Untersuchungen zu den römisch-barbarischen Kontakten Östlich der römischen Provinz Dacia Antiquitatis reihe 3 Abhnadlungen zur vor-und frühgeschichte, zur klassichen und provinzial-römischen archäologie und zur geschichte des altertum

    Directory of Open Access Journals (Sweden)

    Vasile Iarmulschi

    2016-06-01

    Full Text Available Review on book - Alexandru Popa, Untersuchungen zu den römisch-barbarischen Kontakten Östlich der römischen Provinz Dacia Antiquitatis reihe 3 Abhnadlungen zur vor-und frühgeschichte, zur klassichen und provinzial-römischen archäologie und zur geschichte des altertum

  14. A retrospective data acquisition and evaluation on the characterization and prognosis for 776 patients with differentiated thyroid carcinomas in the Klinik und Poliklinik fuer Nuklearmedizin der Universitaet zu Koeln in the period from 1990 to 2005; Eine retrospektive Datenerhebung und Auswertung zur Charakterisierung und Prognose von 776 Patienten mit differenziertem Schilddruesenkarzinom der Klinik und Poliklinik fuer Nuklearmedizin der Universitaet zu Koeln im Zeitraum von 1990-2005

    Energy Technology Data Exchange (ETDEWEB)

    Meixner, Markus

    2011-06-22

    The author describes the retrospective data acquisition and evaluation on the characterization and prognosis for 776 patients (average age 40:1 years) with differentiated thyroid carcinomas in the Klinik und Poliklinik fuer Nuklearmedizin der Universitaet zu Koeln in the period from 1990 to 2005. The methodology includes patient-relevant data, examination data, imaging techniques and biochemical tests. Clinically important differences were found for papillary and follicular thyroid carcinoma. Further differences concern the localization of metastases. The author discusses the prognostic significance of the primary tumor radius in comparison with the so-called TNM classification for papillary and follicular thyroid carcinomas. It is concluded that the new version of the TNM classification offers only slight advantages with respect to the former version. It was also shown that the primary tumor size is adequate for prognostic estimations.

  15. XII All-Russian conference on gas chromatography. Program. Summary of reports

    International Nuclear Information System (INIS)

    2002-01-01

    Program and summary of reports of the XII All-Russian conference on gas chromatography are performed. The conference took place in Samara, 10-14 June, 2002. Reports on physicochemical regularities of gas chromatography, application of chromatographic methods in atomic energetics are included in the program of the conference. Part of the reports are deleted to the analytical application of the gas chromatography. Adsorbents for the gas chromatography, complex methods, mathematic methods in chromatography, ecological aspects of the gas chromatography [ru

  16. Purification of a synthetic pterocarpanquinone by countercurrent chromatography

    International Nuclear Information System (INIS)

    Costa, Fernanda das Neves; Silva, Alcides Jose M. da; Domingos, Jorge L. de Oliveira; Costa, Paulo Roberto R.; Leitao, Gilda G.; Daher Netto, Chaquip

    2012-01-01

    Countercurrent chromatography (CCC) was employed as a useful, fast and economic alternative to conventional chromatography techniques for the purification of a synthetic pterocarpanquinone, LQB-118. The separation was performed in a two-step CCC with the solvent system hexanechloroform- methanol-water 2:1.5:5:2 in both steps. Traditional purification of these reaction products by silica gel column chromatography demanded a large amount of solvent and time, besides allowing the irreversible adsorption of the compound in the column. The use of 1 H NMR for the calculation of KD of target compound is proposed as an alternative for HPLC measurements. (author)

  17. Ion chromatography of anions in the primary and secondary circuit

    International Nuclear Information System (INIS)

    Brandt, F.; Trost, R.

    1984-01-01

    Ion chromatography - though based on the same, well-established basic principles as gas chromatography and high pressure fluid chromatography - has made an actual breakthrough only in recent years. The adaptability of the process permits the measurement of samples of different composition or concentration. Some of the experience which has been accumulated in the laboratory at Goesgen nuclear power station during the last two years, is reported. This relates particularly to the composition of the samples, the need to use extremely pure calibration samples, the choice of special laboratory accessories and the like. (orig.) [de

  18. Planning, budgeting and performance management at Swiss hospitals – Are Swiss hospitals at a crossroads – Will these medical specialist organisations in future develop into institutions with a business orientation? [Die Krankenhäuser der Schweiz am Wendepunkt von der medizinischen Expertenorganisation hin zu betriebswirtschaftlich geführten Institutionen? – Untersuchung zu deren Performance-Management-System

    Directory of Open Access Journals (Sweden)

    Walser, Konrad

    2013-11-01

    die Schweizer Krankenhäuser ein kohärentes und langfristig ausgerichtetes Performance Management aufbauen können. Mit der Einführung von Wettbewerbsmechanismen (DRG-System im neuen Krankenversicherungsgesetz wird die betriebswirtschaftliche Ausrichtung der Krankenhausführung zum Schlüsselfaktor für ein Bestehen am Markt. Die Basis der vorliegenden Untersuchung bildet eine schriftliche Befragung aller Deutschschweizer Krankenhäuser. Diese wurden auf der Grundlage eines Reifegrade-Modells zum Ist- sowie dem Soll-Zustand von deren Performance-Management-Systemen befragt. Das Reifegradmodell wurde für Krankenhäuser angepasst.Die deskriptive Auswertung der Daten zeigt, dass die meisten Krankenhäuser auf dem richtigen Weg sind. Planung und Controlling haben mehrheitlich einen hohen Reifegrad, indes wird die strategische Wichtigkeit eines wirksamen Performance Managements (Reporting, IT-Integration vielerorts noch unterschätzt. So verfügen die meisten Krankenhäuser bereits heute über ausgebaute Planungsmechanismen, beurteilen aber die dazu gehörige Informatikunterstützung als nicht prioritär.Dabei zeigen die bivariaten Auswertungen (Korrelationsmatrizen der Umfrage, dass gerade der IT als Träger oder Plattform des Performance-Managements eine zentrale Rolle zukommt. Gleichzeitig weisen diese weiterführenden Analysen darauf hin, dass die betriebswirtschaftliche Krankenhausplanung der eigentliche Treiber zum Auf- und Ausbau von leistungsfähigen Performance-Management-Systemen ist. Erst eine gut entwickelte und integrierte IT und klare Planwerte, so zeigt sich ausgehend von der Untersuchung, lassen ein umfassendes Performance Management zu.Ein gut funktionierendes Performance Management beinhaltet überdies auch eine organisatorische Komponente. Erst wenn die Aufgaben, Kompetenzen und Verantwortlichkeiten im Hinblick auf die Krankenhaussteuerung bezüglich Betriebswirtschaft, Medizin und Pflege aufeinander abgestimmt sind, können Krankenh

  19. Size exclusion chromatography with superficially porous particles.

    Science.gov (United States)

    Schure, Mark R; Moran, Robert E

    2017-01-13

    A comparison is made using size-exclusion chromatography (SEC) of synthetic polymers between fully porous particles (FPPs) and superficially porous particles (SPPs) with similar particle diameters, pore sizes and equal flow rates. Polystyrene molecular weight standards with a mobile phase of tetrahydrofuran are utilized for all measurements conducted with standard HPLC equipment. Although it is traditionally thought that larger pore volume is thermodynamically advantageous in SEC for better separations, SPPs have kinetic advantages and these will be shown to compensate for the loss in pore volume compared to FPPs. The comparison metrics include the elution range (smaller with SPPs), the plate count (larger for SPPs), the rate production of theoretical plates (larger for SPPs) and the specific resolution (larger with FPPs). Advantages to using SPPs for SEC are discussed such that similar separations can be conducted faster using SPPs. SEC using SPPs offers similar peak capacities to that using FPPs but with faster operation. This also suggests that SEC conducted in the second dimension of a two-dimensional liquid chromatograph may benefit with reduced run time and with equivalently reduced peak width making SPPs advantageous for sampling the first dimension by the second dimension separator. Additional advantages are discussed for biomolecules along with a discussion of optimization criteria for size-based separations. Copyright © 2016 Elsevier B.V. All rights reserved.

  20. Size-exclusion chromatography of perfluorosulfonated ionomers.

    Science.gov (United States)

    Mourey, T H; Slater, L A; Galipo, R C; Koestner, R J

    2011-08-26

    A size-exclusion chromatography (SEC) method in N,N-dimethylformamide containing 0.1 M LiNO(3) is shown to be suitable for the determination of molar mass distributions of three classes of perfluorosulfonated ionomers, including Nafion(®). Autoclaving sample preparation is optimized to prepare molecular solutions free of aggregates, and a solvent exchange method concentrates the autoclaved samples to enable the use of molar-mass-sensitive detection. Calibration curves obtained from light scattering and viscometry detection suggest minor variation in the specific refractive index increment across the molecular size distributions, which introduces inaccuracies in the calculation of local absolute molar masses and intrinsic viscosities. Conformation plots that combine apparent molar masses from light scattering detection with apparent intrinsic viscosities from viscometry detection partially compensate for the variations in refractive index increment. The conformation plots are consistent with compact polymer conformations, and they provide Mark-Houwink-Sakurada constants that can be used to calculate molar mass distributions without molar-mass-sensitive detection. Unperturbed dimensions and characteristic ratios calculated from viscosity-molar mass relationships indicate unusually free rotation of the perfluoroalkane backbones and may suggest limitations to applying two-parameter excluded volume theories for these ionomers. Copyright © 2011 Elsevier B.V. All rights reserved.

  1. [Separation of osteoclasts by lectin affinity chromatography].

    Science.gov (United States)

    Itokazu, M; Tan, A; Tanaka, S

    1991-09-01

    Newborn rat calvaria bone cells obtained by digestion were fractionated on columns of wheat-germ agglutinin (WGA) sepharose 6MB for osteoclast isolation. The initial nonspecific binding cells which were passed through the WGA sepharose column by a buffer acquired a high enzyme activity of alkaline phosphatase, but not that of acid phosphatase. However, elution of cells using a buffer with the addition of N-acetyl-D-glucosamine resulted in a high acid phosphatase activity but no alkaline phosphatase activity. The former WGA binding negative fraction enriched osteoblasts averaging 30 microns in size. The latter WGA binding positive fraction enriched osteoclasts ranging from 20 microns to 60 microns in size. The electron-microscope clearly demonstrated the cellular details of osteoclasts. Isolated cell counts showed a ratio of six to four. These results indicate that our method of osteoclast isolation is simple and useful in lectin affinity chromatography because all cells have sugar moieties on their surface and the binding of osteoclasts can be reversed by the addition of specific lectin-binding sugars to the eluting buffer.

  2. Optimizing heterosurface adsorbent synthesis for liquid chromatography

    Science.gov (United States)

    Bogoslovskii, S. Yu.; Serdan, A. A.

    2016-03-01

    The structural and geometric parameters of a silica matrix (SM) for the synthesis of heterosurface adsorbents (HAs) are optimized. Modification is performed by shielding the external surfaces of alkyl-modified silica (AS) using human serum albumin and its subsequent crosslinking. The structural and geometric characteristics of the SM, AS, and HA are measured via low-temperature nitrogen adsorption. It is found that the structural characteristics of AS pores with diameters D 9 nm reduces significantly due to adsorption of albumin. It is concluded that silica gel with a maximum pore size distribution close to 5 nm and a minimal proportion of pores with D > 9 nm is optimal for HA synthesis; this allows us to achieve the greatest similarity between the chromatographic retention parameters for HA and AS. The suitability of the synthesized adsorbents for analyzing drugs in biological fluids through direct sample injection is confirmed by chromatography. It was found that the percentage of the protein fraction detected at the outlet of the chromatographic column is 98%.

  3. Ion mobility spectrometry after supercritical fluid chromatography

    International Nuclear Information System (INIS)

    Morrissey, M.A.

    1988-01-01

    In this work, a Fourier transform ion mobility spectrometer (FT-IMS) was constructed and evaluated as a detector for supercritical fluid chromatography (SFC). The FT-IMS provides both quantitative and qualitative data of a wide range of compounds, selective and nonselective modes of chromatographic detection, and it is compatible with a wide range of SFC mobile phases. Drift spectra are presented for a number of samples, including polymers, lipids, herbicides, antibiotics, and pharmaceuticals. The unique properties of supercritical fluids made it possible to introduce these compounds into the spectrometer. While the drift spectra presented are generally simple, showing only a quasi-molecular ion, a few are surprising complex. Examples of selective and non-selective detection demonstrate the usefulness of the detector. Examples are presented for fish oil concentrate, bacon grease extract, soil extract, and polymer mixtures. In the case of Triton X-100, a non-ionic surfactant, the FT-IMS was able to selectively detect individual oligomers in the polymer mixture. In the case of a polydimethylsilicone mixture the detector isolated a contaminant in the mixture

  4. Contribution to high-temperature chromatography and high-temperature-gas-chromatography-mass spectrometry of lipids

    International Nuclear Information System (INIS)

    Aichholz, R.

    1998-04-01

    This thesis describes the use of high temperature gas chromatography for the investigation of unusual triacylglycerols, cyanolipids and bees waxes. The used glass capillary columns were pretreated and coated with tailor made synthesized high temperature stable polysiloxane phases. The selective separation properties of the individual columns were tested with a synthetic lipid mixture. Suitable derivatization procedures for the gaschromatographic analyses of neutral lipids, containing multiple bonds as well as hydroxy-, epoxy-, and carboxyl groups, were developed and optimized. Therefore conjugated olefinic-, conjugated olefinic-acetylenic-, hydroxy-, epoxy-, and conjugated olefinic keto triacylglycerols in miscellaneous plant seed oils as well as hydroxy monoesters, diesters and hydroxy diesters in bees waxes could be analysed directly with high temperature gas chromatography for the first time. In order to elucidate the structures of separated lipid compounds, high temperature gas chromatography was coupled to mass spectrometry and tandem mass spectrometry, respectively. Comparable analytical systems are hitherto not commercial available. Therefore instrumental prerequisites for a comprehensive and detailed analysis of seed oils and bees waxes were established. In GC/MS commonly two ionization methods are used, electron impact ionization and chemical ionization. For the analysis of lipids the first is of limited use only. Due to intensive fragmentation only weak molecular ions are observed. In contrast, the chemical ionization yields in better results. Dominant quasi molecular ions enable an unambiguous determination of the molecular weight. Moreover, characteristic fragment ions provide important indications of certain structural features of the examined compounds. Nevertheless, in some cases the chromatographic resolution was insufficient in order to separate all compounds present in natural lipid mixtures. Owing to the selected detection with mass spectrometry

  5. Microbial metabolomics with gas chromatography/mass spectrometry

    NARCIS (Netherlands)

    Koek, M.M.; Muilwijk, B.; Werf, M.J. van der; Hankemeier, T.

    2006-01-01

    An analytical method was set up suitable for the analysis of microbial metabolomes, consisting of an oximation and silylation derivatization reaction and subsequent analysis by gas chromatography coupled to mass spectrometry. Microbial matrixes contain many compounds that potentially interfere with

  6. Microchip-based monolithic column for high performance liquid chromatography

    Data.gov (United States)

    National Aeronautics and Space Administration — We have developed microchip based monolithic columns that can be used for liquid chromatography of small organic molecules, as well as, macromolecules such as...

  7. An Empirical Formula From Ion Exchange Chromatography and Colorimetry.

    Science.gov (United States)

    Johnson, Steven D.

    1996-01-01

    Presents a detailed procedure for finding an empirical formula from ion exchange chromatography and colorimetry. Introduces students to more varied techniques including volumetric manipulation, titration, ion-exchange, preparation of a calibration curve, and the use of colorimetry. (JRH)

  8. Inverse colloidal crystal membranes for hydrophobic interaction membrane chromatography.

    Science.gov (United States)

    Vu, Anh T; Wang, Xinying; Wickramasinghe, S Ranil; Yu, Bing; Yuan, Hua; Cong, Hailin; Luo, Yongli; Tang, Jianguo

    2015-08-01

    Hydrophobic interaction membrane chromatography has gained interest due to its excellent performance in the purification of humanized monoclonal antibodies. The membrane material used in hydrophobic interaction membrane chromatography has typically been commercially available polyvinylidene fluoride. In this contribution, newly developed inverse colloidal crystal membranes that have uniform pores, high porosity and, therefore, high surface area for protein binding are used as hydrophobic interaction membrane chromatography membranes for humanized monoclonal antibody immunoglobulin G purification. The capacity of the inverse colloidal crystal membranes developed here is up to ten times greater than commercially available polyvinylidene fluoride membranes with a similar pore size. This work highlights the importance of developing uniform pore size high porosity membranes in order to maximize the capacity of hydrophobic interaction membrane chromatography. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. High Performance Liquid Chromatography of Vitamin A: A Quantitative Determination.

    Science.gov (United States)

    Bohman, Ove; And Others

    1982-01-01

    Experimental procedures are provided for the quantitative determination of Vitamin A (retinol) in food products by analytical liquid chromatography. Standard addition and calibration curve extraction methods are outlined. (SK)

  10. Plasma wave detection in laser spectroscopy and gas chromatography

    International Nuclear Information System (INIS)

    Franzke, J.; Irmer, A. von; Veza, D.; Niemax, K.

    1995-01-01

    Frequency changes of plasma oscillations in low-pressure discharges are used for sensitive detection of atomic or molecular trace gases. Analyte selectivity can be either obtained by resonant laser excitation or by gas chromatography

  11. Gas Chromatography Mass Spectrometry of Quassia undulata Seed ...

    African Journals Online (AJOL)

    Prof. Ogunji

    The use of gas chromatography mass spectrometry (GC MS) as a sensitive and specific technique ... cold flow properties and stability of the fuel to oxidation, peroxidation and polymerization .... determinants of both the physical and chemical ...

  12. Gas Chromatography-Mass Spectrometric Analysis and Insecticidal ...

    African Journals Online (AJOL)

    HP

    Original Research Article. Gas Chromatography-Mass Spectrometric Analysis and ... into a natural fumigant/insecticide for the control of stored product insects. Keywords: Mallotus ..... stability as well as reduce cost. ACKNOWLEDGEMENT.

  13. A generalized theory of chromatography and multistep liquid extraction

    Science.gov (United States)

    Chizhkov, V. P.; Boitsov, V. N.

    2017-03-01

    A generalized theory of chromatography and multistep liquid extraction is developed. The principles of highly efficient processes for fine preparative separation of binary mixture components on a fixed sorbent layer are discussed.

  14. Gas Chromatography-Mass Spectrometric Analysis of Nematicidal ...

    African Journals Online (AJOL)

    Gas Chromatography-Mass Spectrometric Analysis of Nematicidal Essential Oil of Valeriana ... Tropical Journal of Pharmaceutical Research ... have a potential to be developed to natural nematicides for the control of cereal cyst nematodes.

  15. Gas Chromatography-Mass Spectrometric Analysis and Insecticidal ...

    African Journals Online (AJOL)

    Tropical Journal of Pharmaceutical Research ... apelta aerial parts was analyzed by gas chromatography/mass spectrometric (GC/MS) to determine its composition. ... into a natural fumigant/insecticide for the control of stored product insects.

  16. Optimisation, simulation, and training in ion chromatography - all for free!

    International Nuclear Information System (INIS)

    Dicinoski, G.W.; Shaw, M.J.; Madden, J.E.; Haddad, P.R.

    2001-01-01

    Since its inception in 1975, ion chromatography (IC) has grown to become a powerful separation tool for the analytical chemist. Today, when scientists refer to the term IC, they mean a collection of chromatographic techniques suitable for the separation of inorganic ions and low molecular weight, water-soluble, organic compounds. The range of chromatographic techniques covered by this designation includes: ion-exchange chromatography; reversed-phase ion-interaction chromatography; and ion-exclusion chromatography. The optimization of the IC separation process is extremely time-consuming. A computer simulation software, Virtual Column 2, that accurately mimic retention behaviour in IC, is presented. It is based on large datasets of experimentally measured retention times; an ideal tool for chromatographers

  17. Study of comportment of trioctylphosphine oxide by coat slight chromatography

    International Nuclear Information System (INIS)

    Meddour, Laaldja; Azzouz Abdelkrim

    1996-04-01

    The synthesis and characterisation process of the extractant agent 'Trioctylphosphine oxide' (TOPO) are not very developped in the literature. However, in order to identify this agent (TOPO) in its synthesis process, we attempt several analysis methods. The coat slight chromatography proves the simple and accessible method, that explains the choice of this study. In the present work, we have analysed the TOPO by coat slight chromatography with the intention of finding a better solvent

  18. Ultra-high Performance Liquid Chromatography in Steroid Analysis

    OpenAIRE

    Salonen, Fanny

    2017-01-01

    The latest version of liquid chromatography is ultra-high performance (or pressure) chromatography (UHPLC). In the technique, short and narrow-bore columns with particle sizes below 3 µm are used. The extremely high pressure used results in very short analysis times, excellent separation, and good resolution. This makes UHPLC a good choice for steroidal analysis. Steroids are a highly interesting area of study; they can be recognized as biomarkers for several diseases and are a relevant topic...

  19. Disease specific stress of tumor patients at the beginning of radiotherapy. Effect on psychosocial support requirement; Krankheitsspezifische Belastungen von Tumorpatienten zu Beginn einer Strahlentherapie. Auswirkungen auf den psychosozialen Betreuungsbedarf

    Energy Technology Data Exchange (ETDEWEB)

    Sehlen, S.; Hollenhorst, H.; Schymura, B.; Firsching, M.; Duehmke, E. [Ludwig-Maximilians-Univ., Muenchen (Germany). Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie; Aydemir, U. [Inst. fuer Biometrie und Epidemiologie, Klinikum Grosshadern, Ludwig-Maximilians-Univ., Muenchen (Germany); Herschbach, P. [Technische Univ. Muenchen (Germany). Inst. und Poliklinik fuer Psychosomatische Medizin, Psychotherapie und medizinische Psychologie

    2001-10-01

    requirement. (orig.) [German] Hintergrund: Die Radiotherapie bringt Tumorpatienten in eine besondere Lebenssituation, in der sie eine Reihe von unterschiedlichen Faktoren ausgesetzt werden, deren Auspraegung weitgehend unbekannt ist. Intention dieser Querschnittsstudie war es, erstmals systematisch die spezifischen Belastungen von Tumorpatienten zu Beginn einer Strahlentherapie aufzuzeigen und einen belastungsabhaengigen Betreuungswunsch zu erheben mit dem Ziel einer Belastungsreduktion und Verbesserung von Lebensqualitaet und Compliance waehrend einer radioonkologischen Behandlung. Patienten und Methoden: 732 Patienten wurden zunaechst in die Studie aufgenommen, von denen 446 (60,9%) die Einschlusskriterien erfuellten (Ablehnung 21,0%, Karnofsky-Index 6,6%, organisatorische Probleme 3,4%, Sprachbarrieren 3,0%, kognitive Einschraenkung 2,6%, Tod 2,5%). Folgende Erhebungsinstrumente gingen in die Auswertung zu Beginn der Strahlentherapie ein: medizinische und soziodemographische Daten, Lebenssituation (LS), Fragebogen zur Belastung von Krebspatienten (FBK), selbst definierter Betreuungswunsch (BB). 262 Maenner und 184 Frauen (medianes Alter 60,0 Jahre) mit unterschiedlichen Diagnosen wurden untersucht. Ergebnisse: Auf Skalenebene des Fragebogens zur Belastung von Krebspatienten zeigte sich vor allem eine Belastung durch Einschraenkung der Leistungsfaehigkeit, Angst und Schmerz. Frauen hatten signifikant hoehere Belastungswerte in den Bereichen Schmerz (p=0,016) und Angst (p=0,009), Patienten unter 45 Jahren im Bereich Information (p=0,002) und Patienten zwischen 45 und 60 Jahren in den Bereichen Angst (p=0,002) und Gesamtbelastung (p=0,003). Patientinnen mit einem Mammakarzinom waren am staerksten belastet. Der Anteil von stark belasteten Patienten war in den Skalen Leistungsfaehigkeit (43%) und Angst (40%) am hoechsten. Es bestand ein signifikanter Zusammenhang zwischen hoher Belastung und hohem Betreuungswunsch (insbesondere nach Arzt-/Psychologengespraechen und Information

  20. On open questions of the coalition agreement. EEG levy, national CO{sub 2} levy, electricity tax instead of energy tax?; Zu offenen Fragen des Koalitionsvertrags. EEG-Umlage, nationale CO{sub 2}-Abgabe, Stromsteuer statt Energiesteuer?

    Energy Technology Data Exchange (ETDEWEB)

    Becker, Peter

    2018-03-15

    beschliessen, als Grundlage fuer ein Gesetz. Die Aussagen zur Energiewende enttaeuschen noch mehr. Ein Anteil von etwa 65 Prozent Erneuerbare Energien (EE) bis 2030 wird ''angestrebt''. Wuenschenswert sei die Deckung des ''zusaetzlichen Strombedarfs zur Erreichung der Klimaschutzziele im Verkehr, in Gebaeuden und in der Industrie''; man ahnt: Die haben sich tatsaechlich schon mit der Sektorenkopplung befasst. Aber dass fuer eine Sektorenkopplung, die die Klimaschutzziele zu erreichen erlaubt, eine Verdopplung der Stromproduktion noetig ist, kann man nirgends lesen. Zwar bekennt sich die Politik zu Power to X; auch der Abgeordnete Bareiss von der CDU. Aber dass beispielsweise bei Power to Gas nur etwa eine Effizienz von etwa 30 Prozent erreicht wird, wird von kaum jemand angesprochen. Ein wichtiger Grund fuer die Unverbindlichkeit der Aussagen ist nicht nur der politische Dissens, sondern die hohe Komplexitaet des Stoffs. Z.B.liegt der Zusammenhang zwischen der Einfuehrung einer CO{sub 2}-Abgabe und der Strom- bzw. Energiebesteuerung nicht auf der Hand. Daher soll der Versuch gemacht werden, die Forderungen thesenartig aufzustellen und die Zusammenhaenge kurz zu erlaeutern. Dieser Beitrag ist als Aufforderung zur Diskussion gedacht.

  1. Intercomparison of the measurements of oxalic acid in aerosols by gas chromatography and ion chromatography

    Science.gov (United States)

    Kawamura, Kimitaka; Barrie, Leonard A.; Toom-Sauntry, Desiree

    2010-12-01

    Oxalate, the anion of oxalic acid, is one of the most abundant measurable organic species in atmospheric aerosols. Traditionally, this bifunctional species has been measured by gas chromatography (GC) after derivatization to butyl ester and by ion chromatography (IC) without derivatization. However, there are few published comparisons of the two techniques. Here, we report the results of an intercomparison study for the measurement of oxalic acid in Arctic aerosols (oxalic acid by GC ranged from 6.5-59.1 ng m -3 (av. 26.0 ng m -3, median 26.2 ng m -3) whereas those by IC ranged from 6.6-52.1 ng m -3 (av. 26.6 ng m -3, median 25.4 ng m -3). They showed a good correlation ( r = 0.84) with a slope of 0.96. Thus, observations of oxalate obtained by GC employing dibutyl esters are almost equal to those by IC. Because the accuracy of oxalic acid by GC method largely depends on the method used, it is important to strictly examine the recovery in each study.

  2. Multiphase flow modeling in centrifugal partition chromatography.

    Science.gov (United States)

    Adelmann, S; Schwienheer, C; Schembecker, G

    2011-09-09

    The separation efficiency in Centrifugal Partition Chromatography (CPC) depends on selection of a suitable biphasic solvent system (distribution ratio, selectivity factor, sample solubility) and is influenced by hydrodynamics in the chambers. Especially the stationary phase retention, the interfacial area for mass transfer and the flow pattern (backmixing) are important parameters. Their relationship with physical properties, operating parameters and chamber geometry is not completely understood and predictions are hardly possible. Experimental flow visualization is expensive and two-dimensional only. Therefore we simulated the flow pattern using a volume-of-fluid (VOF) method, which was implemented in OpenFOAM®. For the three-dimensional simulation of a rotating FCPC®-chamber, gravitational centrifugal and Coriolis forces were added to the conservation equation. For experimental validation the flow pattern of different solvent systems was visualized with an optical measurement system. The amount of mobile phase in a chamber was calculated from gray scale values of videos recorded by an image processing routine in ImageJ®. To visualize the flow of the stationary phase polyethylene particles were used to perform a qualitative particle image velocimetry (PIV) analysis. We found a good agreement between flow patterns and velocity profiles of experiments and simulations. By using the model we found that increasing the chamber depth leads to higher specific interfacial area. Additionally a circular flow in the stationary phase was identified that lowers the interfacial area because it pushes the jet of mobile phase to the chamber wall. The Coriolis force alone gives the impulse for this behavior. As a result the model is easier to handle than experiments and allows 3D prediction of hydrodynamics in the chamber. Additionally it can be used for optimizing geometry and operating parameters for given physical properties of solvent systems. Copyright © 2011 Elsevier B

  3. Zur Geschichte der Geowissenschaften im Museum für Naturkunde zu Berlin. Teil 6: Geschichte des Geologisch-Paläontologischen Instituts und Museums der Universität Berlin 1910–2004

    Directory of Open Access Journals (Sweden)

    W. Gross

    2004-01-01

    Full Text Available Die Entwicklung des Geologisch-Paläontologischen Instituts und Museums der Universität Berlin von einer Institution, die Geologie zusammen mit Paläontologie als eine Einheit vertrat, über eine Institution, die eine geotektonische Ausrichtung hatte, zu einer auf Paläontologie konzentrierten Institution wird nachvollzogen. Die beiden Institutsdirektoren am Anfang des 20sten Jahrhunderts waren Vertreter der allumfassenden Geologie des 19ten Jahrhunderts, während die beiden folgenden Direktoren eine Geologie ohne Paläontologie vertraten. Das führte zu einer Trennung der beiden Richtungen, und nach der III. Hochschulreform der DDR 1968 verblieb allein die sammlungsbezogene Paläontologie am Museum. Nach der Wiedervereinigung wurde ein Institut für Paläontologie mit biologischer Ausrichtung mit zwei Professuren, einer für Paläozoologie und einer für Paläobotanik, eingerichtet. The development of the Geologisch-Paläontologisches Institut und Museum of the Museum für Naturkunde at the Humboldt University (formerly Friedrich-Wilhelm-Universität in Berlin from a geology-paleontology institution to a pure paleontology institution is described. The first two directors of the department in the beginning of the 20th century, Prof, von Branca and Prof. Pompeckj, represented a 19th century concept of a geology, which included paleontology, even vertebrate paleontology as the crown jewel of geology. They fought sometimes vigorously against a separation of paleontology from geology. The next two directors. Prof. Stille and Prof, von Bubnoff, were the leading geologists in Germany; to be a student of Stille was a special trade mark in geology of Germany. They represented a geology centered on tectonics. The separation of paleontology as separate section was prepared. The destructions of the Second World War, the following restaurations and the division of Germany into two States influenced strongly their directorships. The education of geologists

  4. Racism, Ethnicity and the Media in Africa: Reflections Inspired by Studies of Xenophobia in Cameroon and South Africa Rassismus, Ethnizität und die Medien in Afrika: Reflektionen angeregt durch Studien zu Fremdenfeindlichkeit in Kamerun und Südafrika

    Directory of Open Access Journals (Sweden)

    Francis B. Nyamnjoh

    2010-01-01

    Full Text Available This paper demonstrates the extent to which the media and belonging in Africa are torn between competing and often conflicting claims of bounded and flexible ideas of culture and identity. It draws on studies of xenophobia in Cameroon and South Africa, inspired by the resilience of the politicization of culture and identity, to discuss the hierarchies and inequalities that underpin political, economic and social citizenship in Africa and the world over, and the role of the media in the production, enforcement and contestation of these hierarchies and inequalities. In any country with liberal democratic aspirations or pretensions, the media are expected to promote national citizenship and its emphasis on large-scale, assimilationist and territorially bounded belonging, while turning a blind eye to those who fall through the cracks as a result of racism and/or ethnicity. Little wonder that such an exclusionary articulation of citizenship is facing formidable challenges from its inherent contradictions and closures, and from an upsurge in the politics of recognition and representation by small-scale communities claiming autochthony at a historical juncture where the rhetoric espouses flexible mobility, postmodern flux and discontinuity. Der vorliegende Beitrag zeigt auf, inwieweit die Medien und gesellschaftliche Bindungen in Afrika zwischen konfligierenden Ansprüchen abgegrenzter und sich wandelnder kultureller Identitäten zerrissen sind. Angeregt durch die Erfahrung der kontinuierlichen Politisierung kultureller und sozialer Identitäten zieht der Autor Studien zu Fremdenfeindlichkeit in Kamerun und Südafrika heran, um die Hierarchien und Ungleichheiten zu diskutieren, auf denen politische, wirtschaftliche und soziale Staatsbürgerschaft in Afrika und darüber hinaus basiert, sowie die Rolle der Medien bei der Entstehung, Verstärkung und im Wettstreit dieser Hierarchien und Ungleichheiten. In jedem liberal-demokratisch ausgerichteten Staat

  5. Analysis of radioactive mixed hazardous waste using derivatization gas chromatography/mass spectrometry, liquid chromatography, and liquid chromatography/mass spectrometry

    International Nuclear Information System (INIS)

    Campbell, J.A.; Lerner, B.D.; Bean, R.M.; Grant, K.E.; Lucke, R.B.; Mong, G.M.; Clauss, S.A.

    1994-08-01

    Six samples of core segments from Tank 101-SY were analyzed for chelators, chelator fragments, and several carboxylic acids by derivatization gas chromatography/mass spectrometry. The major components detected were ethylenediaminetetraacetic acid, nitroso-iminodiacetic acid, nitrilotriacetic acid, citric acid, succinic acid, and ethylenediaminetriacetic acid. The chelator of highest concentration was ethylenediaminetetraacetic acid in all six samples analyzed. Liquid chromatography was used to quantitate low molecular weight acids including oxalic, formic, glycolic, and acetic acids, which are present in the waste as acid salts. From 23 to 61% of the total organic carbon in the samples analyzed was accounted for by these acids

  6. Phytochemical Profile of Erythrina variegata by Using High-Performance Liquid Chromatography and Gas Chromatography-Mass Spectroscopy Analyses

    OpenAIRE

    Suriyavathana Muthukrishnan; Subha Palanisamy; Senthilkumar Subramanian; Sumathi Selvaraj; Kavitha Rani Mari; Ramalingam Kuppulingam

    2016-01-01

    Natural products derived from plant sources have been utilized to treat patients with numerous diseases. The phytochemical constituents present in ethanolic leaf extract of Erythrina variegata (ELEV) were identified by using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectroscopy (GC-MS) analyses. Shade dried leaves were powdered and extracted with ethanol for analyses through HPLC to identify selected flavonoids and through GC-MS to identify other molecules. Th...

  7. Cyborg oder Göttin: Wie Technikforscherinnen ihr Verhältnis zu Technik sehen Cyborg or Goddess: How Women Academics in Science and Technology View their Relationship to Technology

    Directory of Open Access Journals (Sweden)

    Ulrike Kissmann

    2001-11-01

    Full Text Available Die Autorinnen haben für die Beiträge eigene auto/biographische Erlebnisse ausgewählt, in denen der mediale Charakter von Technik deutlich wird. Die Geschichten erzählen also von Technik, die soziale Ordnung und Bedeutung vermittelt und auf diese Weise dazu beiträgt, Geschlechtergrenzen und andere Grenzen wie die zwischen „bekannt“ und „fremd“ oder „normal“ und „anormal“ herzustellen. Die Auto/Biographien sind durch Technik einerseits fremdbestimmt, andererseits zeigen die Autorinnen auch, wie Selbstbestimmung über diese Grenzen hinweg möglich ist. Sie benutzen Donna Haraways Cyborg Metapher (1985, um das Selbst in der Verflechtung von Sozialem und Technischem auto/biographisch zu rekonstruieren.The authors of this book have chosen examples from their individual life stories to illustrate the interpretative character of technology. These stories show how technology mediates social order and meaning and how technology thus contributes to negotiate gendered boundaries and other boundaries, for example, boundaries between “familiar” and “foreign” or “normal” and “abnormal”. The auto/biographies in this book are on the one hand influenced by technology; at the same time, the authors demonstrate that they are also able to transgress these boundaries. Using Haraway’s image of the cyborg (1985 the authors analyse the representation of the self within the interconnections of the social and the technical.

  8. Batteries and accumulators: everything you always wanted to know. Batteries, accumulators and the environment - questions and answers; Batterien und Akkus - Das wollten Sie wissen.. Fragen und Antworten zu Batterien, Akkus und Umwelt

    Energy Technology Data Exchange (ETDEWEB)

    Leuthold, S.; Minkos, A. (comps.)

    2006-07-15

    Batteries and accumulators are part of our everyday life. This brochure answers problems related to their use and disposal, chargers and charging. Starter batteries for motor vehicles are left out of account, as are special batteries e.g. for electric fences or lights on building sites. The brochure also explains the legal fundamentals for disposal of batteries and provides information on environmental problems relating to production and disposal. Frequently asked questions are answered on the final pages. [German] Batterien und Akkus begleiten unseren Alltag. Solange sie die noetige mobile Versorgung ermoeglichen, sind sie gern gesehen. Versagen sie ihren Dienst, beginnen die Probleme: Wohin mit der leeren Batterie, dem nicht mehr funktionsfaehigen Akku (Keinesfalls in den Hausmuell.)? Welches ist das richtige Ladegeraet und das richtige Ladeverfahren fuer den Akku? Zu diesen und anderen Fragen rund um Geraetebatterien und -akkus gibt diese Broschuere Auskunft. Nicht betrachtet werden hier allerdings Starterbatterien (z.B. fuer Autos und Motorraeder) und bestimmte Spezialbatterien (z.B. fuer Weidezaeune oder Baustellenlampen), die einen Sonderfall darstellen. Die Broschuere erlaeutert auch die gesetzlichen Grundlagen fuer die Entsorgung unbrauchbarer Batterien und Akkus und gibt Informationen zur Umweltbelastung durch Herstellung und Entsorgung. Am Ende der Broschuere werden haeufig gestellte Fragen und deren Antworten zusammengestellt.

  9. Fluchen und Flamen, um ein Mann zu sein? Die Verknüpfung von Geschlecht und Sprache durch Vorurteile The Manliness of Swearing and Flaming-On The Role of Stereoptypes in Research on Gender and Language

    Directory of Open Access Journals (Sweden)

    Nicola Döring

    2001-11-01

    Full Text Available Die hier besprochene Monographie der Linguistin Anja Gottburgsen beschäftigt sich theoretisch und empirisch mit sprachbezogenen Geschlechterstereotypen, also mit unseren Vorstellungen darüber, ob und wie Männer und Frauen jeweils in spezifischer und distinkter Weise sprachlich kommunizieren. Im Theorieteil der Arbeit wird der aktuelle Stand der soziolinguistischen und sozialpsychologischen Diskussion zu Geschlecht, Sprachverhalten und Stereotypisierung kritisch referiert und die Bedeutung von sprachbezogenen Geschlechterstereotypen erläutert. Im empirischen Teil berichtet die Autorin die Ergebnisse von drei Fragebogen-Studien mit deutschsprachigen Studierenden. Es zeigt sich, dass bei Studierenden unabhängig von Geschlecht und Geschlechtsrollenorientierung stereotype Vorstellungen über das sprachliche Verhalten von Männern und Frauen verbreitet sind.Linguist Gottburgsen presents both a theoretical and empirical analysis of speech-related gender stereotypes -of common ideas about “male” and “female” ways of speaking. Gottburgsen takes a critical look at current socio-linguistic and social-psychological literature on gender, language behaviour and stereotyping and explains the meaningof language-related gender stereotypes. She furthermore presents the results of three survey studies of German-speaking university students. According to these studies, university students, regardless of sex and of their ideas about gender roles, continue to have quite stereotypical views on “gender-appropriate” language behaviour.

  10. Comprehensive two-dimensional liquid chromatography and hyphenated liquid chromatography to study the degradation of poly(bisphenol A)carbonate

    NARCIS (Netherlands)

    Coulier, L.; Kaal, E.R.; Hankemeier, T.

    2005-01-01

    Size exclusion chromatography (SEC), gradient polymer elution chromatography (GPEC) and liquid chromatography at critical conditions (LC-CC) have been developed and applied to observe chemical changes in poly(bisphenol A)carbonate (PC) due to hydrolytic degradation. Especially LC-CC appeared to be

  11. Towards early detection of the hydrolytic degradation of poly(bisphenol A)carbonate by hyphenated liquid chromatography and comprehensive two-dimensional liquid chromatography

    NARCIS (Netherlands)

    Coulier, L.; Kaal, E.R.; Hankemeier, Th.

    2006-01-01

    The hydrolytic degradation of poly(bisphenol A)carbonate (PC) has been characterized by various liquid chromatography techniques. Size exclusion chromatography (SEC) showed a significant decrease in molecular mass as a result of hydrolytic degradation, while 'liquid chromatography at critical

  12. Determination of Pb-210 and actinides by extraction chromatography and anion exchange chromatography

    International Nuclear Information System (INIS)

    Kalmykov, St.N.; Sapozhnikov, Yu.A.

    1997-01-01

    This work is devoted to the determination of Pb-210 and actinides (Pu-238, Pu-239, Am-241, U-235, U-238, Th-232) by means of highly selective chromatographic resins and anion exchangers. The special interest was paid to the analysis of large quantities of samples with high concentration of competitive ions like ocean sediments, bone ash and others.The commercially available TRU-Spec chromatographic resins was used for separation of actinides from the matrix. Then U, Th, Am, and Pu were separated from other using anion exchange chromatography with AG-1X4 anionite in Cl - form, electro-deposed and α-counted.Pb-21- and Bi-210 were determined by liquid scintillation counting. The developed procedure is rather express, effective and could be adopted for the determination of radionuclides like Ba-133, Ra, Np-239

  13. Affinity monolith chromatography: A review of general principles and applications.

    Science.gov (United States)

    Li, Zhao; Rodriguez, Elliott; Azaria, Shiden; Pekarek, Allegra; Hage, David S

    2017-11-01

    Affinity monolith chromatography, or AMC, is a liquid chromatographic method in which the support is a monolith and the stationary phase is a biological-binding agent or related mimic. AMC has become popular for the isolation of biochemicals, for the measurement of various analytes, and for studying biological interactions. This review will examine the principles and applications of AMC. The materials that have been used to prepare AMC columns will be discussed, which have included various organic polymers, silica, agarose, and cryogels. Immobilization schemes that have been used in AMC will also be considered. Various binding agents and applications that have been reported for AMC will then be described. These applications will include the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, dye-ligand affinity chromatography, and immobilized metal-ion affinity chromatography. The use of AMC with chiral stationary phases and as a tool to characterize biological interactions will also be examined. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. [Column chromatography purification and analysis of biodiesel by transesterification].

    Science.gov (United States)

    Liu, Yang; Yi, Huai-feng; Chen, Yu; Wu, Yu-long; Yang, Ming-de; Chen, Zeng; Tong, Jun-mao

    2012-02-01

    In the present paper, crude biodiesel prepared with sorbifolia oil as raw material by transesterification was purified by column chromatography, then the composition of biodiesel was analyzed by gas chromatography, FTIR, GC-MS and 1H NMR. Column chromatography can separate the crude biodiesel into two fractions: petroleum ether eluted fraction (A1) and methanol eluted fraction (A2). Petroleum ether eluted fraction was mainly biodiesel fraction, which was produced from sorbifolia oil by transesterification, including methyl linoleate, methyl cis-9-octadecenoate and so on; methanol eluted fraction was mainly glycerol fraction, which came from the side reaction of transesterification. The results show that the purity of refined biodiesel increased from 77.51% to 93.872, and the product recovery rate reached up to 91.04% after the purification by column chromatography. The results obtained by FTIR and 1H NMR further showed that the column chromatography can effectively improve the purity of biodiesel. This paper provides a basis for industrialization of purification of biodiesel.

  15. Carbon nanotube based stationary phases for microchip chromatography

    DEFF Research Database (Denmark)

    Mogensen, Klaus Bo; Kutter, Jörg Peter

    2012-01-01

    already been demonstrated in more classical formats, for improved separation performance in gas and liquid chromatography, and for unique applications in solid phase extraction. Carbon nanotubes are now also entering the field of microfluidics, where there is a large potential to be able to provide......The objective of this article is to provide an overview and critical evaluation of the use of carbon nanotubes and related carbon-based nanomaterials for microchip chromatography. The unique properties of carbon nanotubes, such as a very high surface area and intriguing adsorptive behaviour, have...... integrated, tailor-made nanotube columns by means of catalytic growth of the nanotubes inside the fluidic channels. An evaluation of the different implementations of carbon nanotubes and related carbon-based nanomaterials for microfluidic chromatography devices is given in terms of separation performance...

  16. Diamond based adsorbents and their application in chromatography.

    Science.gov (United States)

    Peristyy, Anton A; Fedyanina, Olga N; Paull, Brett; Nesterenko, Pavel N

    2014-08-29

    The idea of using diamond and diamond containing materials in separation sciences has attracted a strong interest in the past decade. The combination of a unique range of properties, such as chemical inertness, mechanical, thermal and hydrolytic stability, excellent thermal conductivity with minimal thermal expansion and intriguing adsorption properties makes diamond a promising material for use in various modes of chromatography. This review summarises the recent research on the preparation of diamond and diamond based stationary phases, their properties and chromatographic performance. Special attention is devoted to the dominant retention mechanisms evident for particular diamond containing phases, and their subsequent applicability to various modes of chromatography, including chromatography carried out under conditions of high temperature and pressure. Copyright © 2014 Elsevier B.V. All rights reserved.

  17. Size-exclusion chromatography using core-shell particles.

    Science.gov (United States)

    Pirok, Bob W J; Breuer, Pascal; Hoppe, Serafine J M; Chitty, Mike; Welch, Emmet; Farkas, Tivadar; van der Wal, Sjoerd; Peters, Ron; Schoenmakers, Peter J

    2017-02-24

    Size-exclusion chromatography (SEC) is an indispensable technique for the separation of high-molecular-weight analytes and for determining molar-mass distributions. The potential application of SEC as second-dimension separation in comprehensive two-dimensional liquid chromatography demands very short analysis times. Liquid chromatography benefits from the advent of highly efficient core-shell packing materials, but because of the reduced total pore volume these materials have so far not been explored in SEC. The feasibility of using core-shell particles in SEC has been investigated and contemporary core-shell materials were compared with conventional packing materials for SEC. Columns packed with very small core-shell particles showed excellent resolution in specific molar-mass ranges, depending on the pore size. The analysis times were about an order of magnitude shorter than what could be achieved using conventional SEC columns. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Liquid-adsorption chromatography of metal 8-hydroxyquinolinates

    International Nuclear Information System (INIS)

    Basova, E.M.; Bol'shova, T.A.; Shirshova, T.S.

    1988-01-01

    Using the method of liquid-adsorption chromatography separation of 8-hydroxyquinolinates of Cu, Fe, Ga, In, Al, Ni is realized. Thin-layer chromatography (TLC) is used to define the optimum conditions of separation and highly efficient liquid column chromatography (HELC) - for chelate separation. Applicaton of the same sorbent is a very important condition of transition from TLC to HELC. Solica gel Partisil, mobile phase: benzene - isopropanol mixture, containing 1 x 10 -3 M of 8-hydroxyquinoline, are the optimal conditions for the studied chelates separation. But In and Ga in TLC move together, and in HELC they are partially separated, therefore, for their separation the column selectivity should be improved by selection of mobile phase

  19. Application of ion chromatography in clinical studies and pharmaceutical industry.

    Science.gov (United States)

    Michalski, Rajmund

    2014-01-01

    Ion chromatography is a well-established regulatory method for analyzing anions and cations in environmental, food and many other samples. It offers an enormous range of possibilities for selecting stationary and mobile phases. Additionally, it usually helps to solve various separation problems, particularly when it is combined with different detection techniques. Ion chromatography can also be used to determine many ions and substances in clinical and pharmaceutical samples. It provides: availability of high capacity stationary phases and sensitive detectors; simple sample preparation; avoidance of hazardous chemicals; decreased sample volumes; flexible reaction options on a changing sample matrix to be analyzed; or the option to operate a fully-automated system. This paper provides a short review of the ion chromatography applications for determining different inorganic and organic substances in clinical and pharmaceutical samples.

  20. Ion-Exchange Chromatography: Basic Principles and Application.

    Science.gov (United States)

    Cummins, Philip M; Rochfort, Keith D; O'Connor, Brendan F

    2017-01-01

    Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful resolving ability, and ease of scale-up and automation have led to it becoming one of the most versatile and widely used of all liquid chromatography (LC) techniques. In this chapter, we review the basic principles of IEC, as well as the broader criteria for selecting IEC conditions. By way of further illustration, we outline basic laboratory protocols to partially purify a soluble serine peptidase from bovine whole brain tissue, covering crude tissue extract preparation through to partial purification of the target enzyme using anion-exchange chromatography. Protocols for assaying total protein and enzyme activity in both pre- and post-IEC fractions are also described.

  1. Radiogas chromatography mass spectrometry in the selected ion monitoring mode

    International Nuclear Information System (INIS)

    Doerfler, D.L.; Rosenblum, E.R.; Malloy, J.M.; Naworal, J.D.; McManus, I.R.; Campbell, I.M.

    1980-01-01

    The value of selected ion monitoring in analyzing biological radio isotope incorporation experiments by radiogas chromatography mass spectrometry is illustrated with reference to the biosynthesis of the mycotoxin mycophenolic acid in Penicillium brevicompactum and the mode of action of the anticholesterolemic drug 20,25-diazacholesterol. Both examples used 1-[ 14 C]acetate precursors. It is shown that the increased sensitivity and specificity of the selected ion monitoring mode detector permits straightforward detection and identification of the relatively small cellular pools associated with metabolic intermediates. The computer program RADSIM is described. Problems that still exist in using radiogas gas chromatography mass spectrometry technology to analyse isotope incorporation experiments are discussed. (author)

  2. Forced-flow chromatography of rare earths using sensitive spectrophometry

    International Nuclear Information System (INIS)

    Matsui, Masakazu; Aoki, Toru; Kumagai, Tetsu.

    1981-01-01

    The sensitive spectrophotometric method for the rare earth elements with xylenol orange in the presence of cetylpyridinium bromide was applied to the continuous detection system of liquid chromatography. Fourteen rare earth elements were completely separated within 130 min cation-exchange chromatography using 2-hydroxy-iso-butylic acid. The eluted ions were determined with absorption maxima of their complexes at around 610 nm. A linear relationship between the peak height and the amounts of rare earth elements was also obtained over the range 0.04 to 0.5 MU g. (author)

  3. Modeling of biopharmaceutical processes. Part 2: Process chromatography unit operation

    DEFF Research Database (Denmark)

    Kaltenbrunner, Oliver; McCue, Justin; Engel, Philip

    2008-01-01

    Process modeling can be a useful tool to aid in process development, process optimization, and process scale-up. When modeling a chromatography process, one must first select the appropriate models that describe the mass transfer and adsorption that occurs within the porous adsorbent. The theoret......Process modeling can be a useful tool to aid in process development, process optimization, and process scale-up. When modeling a chromatography process, one must first select the appropriate models that describe the mass transfer and adsorption that occurs within the porous adsorbent...

  4. Early stages in the history of gas chromatography.

    Science.gov (United States)

    Kolomnikov, Ivan G; Efremov, Alexander M; Tikhomirova, Tatyana I; Sorokina, Nadezhda M; Zolotov, Yury A

    2018-02-16

    The creation of gas chromatography is traditionally associated with the names of Nobel Prize winner Archer Martin and his colleagues Richard Synge and Anthony James. However, sometimes references to their predecessors can be found. An investigation conducted by the authors of this article not only confirmed the reliability of these references; but in fact led to the conclusion that by 1952, which is commonly believed to be the year when gas chromatography was born, many research papers had already been devoted to this method, mainly, in its gas-solid version. These papers are considered in this article. Copyright © 2018. Published by Elsevier B.V.

  5. Fractionation of fecal neutral steroids by high performance liquid chromatography

    International Nuclear Information System (INIS)

    Jackson, E.M.; Kloss, C.A.; Weintraub, S.T.; Mott, G.E.

    1985-01-01

    Fecal neutral steroids were fractionated by high performance liquid chromatography (HPLC) into three major fractions: 5 beta-H, 3-keto steroids; 5 beta-H, 3 beta-hydroxy steroids; and 5 alpha-H and delta 5-3 beta-hydroxy steroids. This separation was achieved in about 10 minutes, with greater than 97% recovery of standards in each fraction. Gas-liquid chromatographic quantitation of fecal steroids fractionated by either HPLC or thin-layer chromatography gave nearly identical results. A method using both C18 reverse phase and silica HPLC to purify radiolabeled sterols is also described

  6. Application of genetic algorithms for parameter estimation in liquid chromatography

    International Nuclear Information System (INIS)

    Hernandez Torres, Reynier; Irizar Mesa, Mirtha; Tavares Camara, Leoncio Diogenes

    2012-01-01

    In chromatography, complex inverse problems related to the parameters estimation and process optimization are presented. Metaheuristics methods are known as general purpose approximated algorithms which seek and hopefully find good solutions at a reasonable computational cost. These methods are iterative process to perform a robust search of a solution space. Genetic algorithms are optimization techniques based on the principles of genetics and natural selection. They have demonstrated very good performance as global optimizers in many types of applications, including inverse problems. In this work, the effectiveness of genetic algorithms is investigated to estimate parameters in liquid chromatography

  7. Prostate cell membrane chromatography-liquid chromatography-mass spectrometry for screening of active constituents from Uncaria rhynchophylla.

    Science.gov (United States)

    He, Jianyu; Han, Shengli; Yang, Fangfang; Zhou, Nan; Wang, Sicen

    2013-01-01

    Uncaria rhynchophylla is a traditional Chinese medicinal herb used to treat hypertension and convulsive disorders such as epilepsy. Rat prostate cell membrane chromatography combined with liquid chromatography-mass spectrometry (LC-MS) was used to identify active constituents from U. rhynchophylla extracts. Four compounds (corynoxeine, isorhynchophylline, isocorynoxeine and rhynchophylline) were discovered. Competitive binding assay results indicated that the four compounds were in direct competition at a single common binding site and interacted with α1A adrenergic receptors (α1A-AR) in a manner similar to tamsulosin. Affinity constant values of the four compounds binding with α1A-AR were also measured using rat prostate cell membrane chromatography (CMC). Finally, their pharmacodynamic effects were tested on rat caudal arteries. This CMC combined LC-MS system offers a means of drug discovery by screening natural medicinal herbs for new pharmacologically active molecules targeting specific receptors.

  8. Liquid Chromatography Applied to Space System

    Science.gov (United States)

    Poinot, Pauline; Chazalnoel, Pascale; Geffroy, Claude; Sternberg, Robert; Carbonnier, Benjamin

    , etc.) which are good examples for one of the two intrinsic features of life (i.e. complexity) would then be searched for. Although these methods are very promising as they have already demonstrated real benefits in terms of sensitivity towards specific compounds of middle/high molecular weight, they cannot be used to detect in one pot a wide range of biopolymer targets with very diverse nature, such as peptides or oligonucleotides. In this context, it would be interesting to develop a “micro-lab” equipped with a miniaturized HPLC-MS as the ones currently developed in the field of biological and medicinal sciences. The objective is to demonstrate unequivocally the presence or absence in space of a wide range of biopolymers thanks to a “one step one pot” instrumentation. We propose to demonstrate the feasibility and the validity of such a concept. For that, we optimize the chromatographic conditions and the mass spectrometer parameters to detect in the range of ppb, proteins and polypeptides biomarkers, while taking into account the space constraints. On a UPLC-HRMS (Q-Exactive and Qq-TOF), different stationary phases (laboratory-made or commercially available), different eluents, gradient flows, temperatures, pressures, and the use of a pre-concentration stage are tested. Dual detection (MS and diode array) is also considered. First experiments have highlighted the ability of such a technique to find ultra-traces level of organic matters under definite space constraints (elution flow, solvents, temperature...). This work is funded by the French Space Agency (CNES) References Glavin DP, Schubert Ml, Botta O, Kminek G, Bada JL (2001) Detecting pyrolysis products from bacteria on Mars. Earth Planet Sc Lett 185:1-2. doi:10.1016/S0012-821X(00)00370-8 Navarro-González R, Navarro KF, de la Rosa J, Iñliguez E, Molina P, Mira LD (2006) The limitations on organic detection in Mars-like soils by thermal volatilization-gas chromatography-MS and their implications for the

  9. Value of breast MRI as supplement to mammography and sonography for high risk breast cancer patients; Wertigkeit der Mamma-MRT als Ergaenzung zu Mammographie und Sonographie bei Patientinnen mit erhoehtem Mammakarzinomrisiko

    Energy Technology Data Exchange (ETDEWEB)

    Schlossbauer, T.; Hellerhoff, K.; Reiser, M. [Klinikum Grosshadern der Ludwig-Maximilians-Universitaet Muenchen, Institut fuer Klinische Radiologie, Muenchen (Germany)

    2008-04-15

    The aim of this study is to give an overview on early detection of breast cancer in patients with an increased risk of breast cancer. Sensitivities and diagnostic accuracies of breast MRI, mammography and ultrasound were compared. A systematic literature search of the past 3 years was performed. Studies which compared breast imaging modalities and used image-guided biopsy results as standard of reference were included. Patients included had to have had an increased lifetime risk for breast cancer (>15%). Regarding sensitivity and diagnostic accuracy, breast MRI performed best in comparison to the other modalities within this collective of patients. Sensitivities ranged from 71-100%, 0-78%, and 13-65%, for MRI, mammography, and ultrasound, respectively Breast MRI is a well established tool for screening in patients at high risk for developing breast cancer and is a valuable supplement to mammography and ultrasound within this selected cohort of patients. (orig.) [German] Ziele der Arbeit sind die inhaltliche Einfuehrung in das Thema Frueherkennung des Mammakarzinoms bei Patientinnen mit erhoehtem Risiko und eine systematische Analyse der Wertigkeit von MRT und anderen bildgebenden Verfahren in diesem Zusammenhang. Es wurde eine systematische Literaturrecherche ueber die letzten 3 Jahre durchgefuehrt. Dabei wurden Vergleichsstudien der verschiedenen bildgebenden Verfahren in der Mammadiagnostik beruecksichtigt, bei denen die bildgesteuerte Biopsie als Goldstandard angegeben wurde. Von allen bildgebenden Verfahren zeigt die Mamma-MRT bei Patientinnen mit familiaerem Risiko die hoechste Sensitivitaet und diagnostische Genauigkeit bei der Detektion des Mammakarzinoms. Fuer MRT, Mammographie und Sonographie werden Sensitivitaeten zwischen 71 und 100%, 0 und 78% sowie 13 und 65% angegeben. Die Mamma-MRT ist ein etabliertes Verfahren zum Screening von Risikopatientinnen. Uebereinstimmend wird der diagnostische Nutzen der Mamma-MRT als ergaenzendes Verfahren zu Mammographie

  10. Schinus terebinthifolius countercurrent chromatography (Part III): Method transfer from small countercurrent chromatography column to preparative centrifugal partition chromatography ones as a part of method development.

    Science.gov (United States)

    das Neves Costa, Fernanda; Hubert, Jane; Borie, Nicolas; Kotland, Alexis; Hewitson, Peter; Ignatova, Svetlana; Renault, Jean-Hugues

    2017-03-03

    Countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC) are support free liquid-liquid chromatography techniques sharing the same basic principles and features. Method transfer has previously been demonstrated for both techniques but never from one to another. This study aimed to show such a feasibility using fractionation of Schinus terebinthifolius berries dichloromethane extract as a case study. Heptane - ethyl acetate - methanol -water (6:1:6:1, v/v/v/v) was used as solvent system with masticadienonic and 3β-masticadienolic acids as target compounds. The optimized separation methodology previously described in Part I and II, was scaled up from an analytical hydrodynamic CCC column (17.4mL) to preparative hydrostatic CPC instruments (250mL and 303mL) as a part of method development. Flow-rate and sample loading were further optimized on CPC. Mobile phase linear velocity is suggested as a transfer invariant parameter if the CPC column contains sufficient number of partition cells. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Nanoparticle Analysis by Online Comprehensive Two-Dimensional Liquid Chromatography combining Hydrodynamic Chromatography and Size-Exclusion Chromatography with Intermediate Sample Transformation

    Science.gov (United States)

    2017-01-01

    Polymeric nanoparticles have become indispensable in modern society with a wide array of applications ranging from waterborne coatings to drug-carrier-delivery systems. While a large range of techniques exist to determine a multitude of properties of these particles, relating physicochemical properties of the particle to the chemical structure of the intrinsic polymers is still challenging. A novel, highly orthogonal separation system based on comprehensive two-dimensional liquid chromatography (LC × LC) has been developed. The system combines hydrodynamic chromatography (HDC) in the first-dimension to separate the particles based on their size, with ultrahigh-performance size-exclusion chromatography (SEC) in the second dimension to separate the constituting polymer molecules according to their hydrodynamic radius for each of 80 to 100 separated fractions. A chip-based mixer is incorporated to transform the sample by dissolving the separated nanoparticles from the first-dimension online in tetrahydrofuran. The polymer bands are then focused using stationary-phase-assisted modulation to enhance sensitivity, and the water from the first-dimension eluent is largely eliminated to allow interaction-free SEC. Using the developed system, the combined two-dimensional distribution of the particle-size and the molecular-size of a mixture of various polystyrene (PS) and polyacrylate (PACR) nanoparticles has been obtained within 60 min. PMID:28745485

  12. Carbon monoxide measurement by gas chromatography; Mesure du monoxyde de carbone par chromatographie en phase gazeuse

    Energy Technology Data Exchange (ETDEWEB)

    Gros, V.; Sarda-Esteve, R.; Bonsang, B.; Ramonet, M.

    1998-09-01

    Although carbon monoxide (CO) is present in trace quantities in the atmosphere (0.1 ppm -or parts per million in volume- on average), the study of this gas is important. Indeed, its impact on human can be dangerous at high level of concentration on the hand and it constitutes one of the main precursor of ozone in presence of concentration on the one hand and it constitutes one of the main precursor of ozone in presence of other pollutants on the other hand. Finally, CO affects the levels of several important greenhouse gases, through its reaction with hydroxyl radicals (OH). CO is measured in the atmosphere since the mid 60's by various methods. Among them, gas chromatography has the advantage to combine a low detection limit with a high precision. This report details the improvements made on the measurement analyser which allowed to perform automatic CO measurements in remote areas with low mixing ratios of carbon monoxide. This report describes some quality tests and the results of various applications. (authors)

  13. Liquid chromatography and liquid chromatography-mass spectrometry analysis of donepezil degradation products

    Directory of Open Access Journals (Sweden)

    Mladenović Aleksandar R.

    2015-01-01

    Full Text Available This study describes the investigation of degradation products of donepezil (DP using stability indicating RP-HPLC method for determination of donepezil, which is a centrally acting reversible acetylcholinesterase inhibitor. In order to investigate the stability of drug and formed degradation products, a forced degradation study of drug sample and finished product under different forced degradation conditions has been conducted. Donepezil hydrochloride and donepezil tablets were subjected to stress degradation conditions recommended by International Conference on Harmonization (ICH. Donepezil hydrochloride solutions were subjected to acid and alkali hydrolysis, chemical oxidation and thermal degradation. Significant degradation was observed under alkali hydrolysis and oxidative degradation conditions. Additional degradation products were observed under the conditions of oxidative degradation. The degradation products observed during forced degradation studies were monitored using the high performance liquid chromatography (HPLC method developed. The parent method was modified in order to obtain LC-MS compatible method which was used to identify the degradation products from forced degradation samples using high resolution mass spectrometry. The mass spectrum provided the precise mass from which derived molecular formula of drug substance and degradation products formed and proved the specificity of the method unambiguously. [Projekat Ministarstva nauke Republike Srbije, br. 172013

  14. Comparison of Membrane Chromatography and Monolith Chromatography for Lactoferrin and Bovine Serum Albumin Separation

    Directory of Open Access Journals (Sweden)

    Chalore Teepakorn

    2016-09-01

    Full Text Available These last few decades, membranes and monoliths have been increasingly used as stationary phases for chromatography. Their fast mass transfer is mainly based on convection, which leads to reduced diffusion, which is usually observed in resins. Nevertheless, poor flow distribution, which causes inefficient binding, remains a major challenge for the development of both membrane and monolith devices. Moreover, the comparison of membranes and monoliths for biomolecule separation has been very poorly investigated. In this paper, the separation of two proteins, bovine serum albumin (BSA and lactoferrin (LF, with similar sizes, but different isoelectric points, was investigated at a pH of 6.0 with a BSA-LF concentration ratio of 2/1 (2.00 mg·mL−1 BSA and 1.00 mg·mL−1 LF solution using strong cation exchange membranes and monoliths packed in the same housing, as well as commercialized devices. The feeding flow rate was operated at 12.0 bed volume (BV/min for all devices. Afterward, bound LF was eluted using a phosphate-buffered saline solution with 2.00 M NaCl. Using membranes in a CIM housing from BIA Separations (Slovenia with porous frits before and after the membrane bed, higher binding capacities, sharper breakthrough curves, as well as sharper and more symmetric elution peaks were obtained. The monolith and commercialized membrane devices showed lower LF binding capacity and broadened and non-symmetric elution peaks.

  15. Adaptation to high throughput batch chromatography enhances multivariate screening.

    Science.gov (United States)

    Barker, Gregory A; Calzada, Joseph; Herzer, Sibylle; Rieble, Siegfried

    2015-09-01

    High throughput process development offers unique approaches to explore complex process design spaces with relatively low material consumption. Batch chromatography is one technique that can be used to screen chromatographic conditions in a 96-well plate. Typical batch chromatography workflows examine variations in buffer conditions or comparison of multiple resins in a given process, as opposed to the assessment of protein loading conditions in combination with other factors. A modification to the batch chromatography paradigm is described here where experimental planning, programming, and a staggered loading approach increase the multivariate space that can be explored with a liquid handling system. The iterative batch chromatography (IBC) approach is described, which treats every well in a 96-well plate as an individual experiment, wherein protein loading conditions can be varied alongside other factors such as wash and elution buffer conditions. As all of these factors are explored in the same experiment, the interactions between them are characterized and the number of follow-up confirmatory experiments is reduced. This in turn improves statistical power and throughput. Two examples of the IBC method are shown and the impact of the load conditions are assessed in combination with the other factors explored. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Column Chromatography To Obtain Organic Cation Sorption Isotherms.

    Science.gov (United States)

    Jolin, William C; Sullivan, James; Vasudevan, Dharni; MacKay, Allison A

    2016-08-02

    Column chromatography was evaluated as a method to obtain organic cation sorption isotherms for environmental solids while using the peak skewness to identify the linear range of the sorption isotherm. Custom packed HPLC columns and standard batch sorption techniques were used to intercompare sorption isotherms and solid-water sorption coefficients (Kd) for four organic cations (benzylamine, 2,4-dichlorobenzylamine, phenyltrimethylammonium, oxytetracycline) with two aluminosilicate clay minerals and one soil. A comparison of Freundlich isotherm parameters revealed isotherm linearity or nonlinearity was not significantly different between column chromatography and traditional batch experiments. Importantly, skewness (a metric of eluting peak symmetry) analysis of eluting peaks can establish isotherm linearity, thereby enabling a less labor intensive means to generate the extensive data sets of linear Kd values required for the development of predictive sorption models. Our findings clearly show that column chromatography can reproduce sorption measures from conventional batch experiments with the benefit of lower labor-intensity, faster analysis times, and allow for consistent sorption measures across laboratories with distinct chromatography instrumentation.

  17. Specialized Gas Chromatography--Mass Spectrometry Systems for Clinical Chemistry.

    Science.gov (United States)

    Gochman, Nathan; And Others

    1979-01-01

    A discussion of the basic design and characteristics of gas chromatography-mass spectrometry systems used in clinical chemistry. A comparison of three specific systems: the Vitek Olfax IIA, Hewlett-Packard HP5992, and Du Pont DP-102 are included. (BB)

  18. Determination of Caffeine in Beverages by High Performance Liquid Chromatography.

    Science.gov (United States)

    DiNunzio, James E.

    1985-01-01

    Describes the equipment, procedures, and results for the determination of caffeine in beverages by high performance liquid chromatography. The method is simple, fast, accurate, and, because sample preparation is minimal, it is well suited for use in a teaching laboratory. (JN)

  19. Beta-induced fluorescence detection in liquid chromatography

    International Nuclear Information System (INIS)

    Malcolme-Lawes, D.J.; Massey, S.; Warwick, P.

    1981-01-01

    A theoretical analysis of beta-induced fluorescence is used to determine the factors which influence the sensitivity of the technique as applied to liquid chromatography. Equations are presented for detector response and for signal-to-noise ratios and the theoretical response for a typical detector is compared with experimentally determined values. (author)

  20. Pre-staining thin layer chromatography method for amino acid ...

    African Journals Online (AJOL)

    Jane

    2010-12-13

    Dec 13, 2010 ... inexpensive and the results obtained were clean and reproducible. However, it is suitable for the high throughput screening of amino acid-producing strains. Key words: Thin layer chromatography, pre-staining, amino acid detection. INTRODUCTION. Several analytical techniques have been often used for.

  1. Separation of zirconium and hafnium using paper distribution chromatography

    International Nuclear Information System (INIS)

    Lebedeva, G.G.; Viktorova, M.E.

    1981-01-01

    A method is suggested of chromatographic separation of zirconium and hafnium in a CCl 4 -tributyl phosphate system (1:9) containing KCl as a salting-out agent in 5 M HNO 3 at 28-30 deg C. Zr and Hf are deterfmined in articiial mixtures under optimal chromatography conditions using visual colorimetry [ru

  2. Gas chromatography mass spectrometry : key technology in metabolomics

    NARCIS (Netherlands)

    Koek, Maud Marijtje

    2009-01-01

    Metabolomics involves the unbiased quantitative and qualitative analysis of the complete set of metabolites present in cells, body fluids and tissues. Gas chromatography coupled to mass spectrometry (GC-MS) is very suitable for metabolomics analysis, as it combines high separation power with

  3. Extending the molecular application range of gas chromatography

    NARCIS (Netherlands)

    Kaal, E.; Janssen, H.-G.

    2008-01-01

    Gas chromatography is an important analytical technique for qualitative and quantitative analysis in a wide range of application areas. It is fast, provides a high peak capacity, is sensitive and allows combination with a wide range of selective detection methods including mass spectrometry.

  4. Supercritical fluid chromatography of fish, shark and seal oils

    DEFF Research Database (Denmark)

    Borch-Jensen, Christina; Mollerup, Jørgen

    1996-01-01

    Various natural and treated fish, shark liver and seal oils have been analyzed by supercritical fluid chromatography (SFC) using a non-polar capillary column. The lipids are separated according to molecular mass. The lipid groups found included free fatty acids, cholesterol, squalene, vitamins, wax...... applications of SFC on fish, seal and shark liver oils are presented....

  5. Analysis of moniliformin in maize plants using hydrophilic interaction chromatography

    DEFF Research Database (Denmark)

    Sørensen, Jens Laurids; Nielsen, Kristian Fog; Thrane, Ulf

    2007-01-01

    A novel HPLC method was developed for detection of the Fusarium mycotoxin, moniliformin in whole maize plants. The method is based on hydrophilic interaction chromatography (HILIC) on a ZIC zwitterion column combined with diode array detection and negative electrospray mass spectrometry (ESI...

  6. Choices of capture chromatography technology in antibody manufacturing processes.

    Science.gov (United States)

    DiLeo, Michael; Ley, Arthur; Nixon, Andrew E; Chen, Jie

    2017-11-15

    The capture process employed in monoclonal antibody downstream purification is not only the most critically impacted process by increased antibody titer resulting from optimized mammalian cell culture expression systems, but also the most important purification step in determining overall process throughput, product quality, and economics. Advances in separation technology for capturing antibodies from complex feedstocks have been one focus of downstream purification process innovation for past 10 years. In this study, we evaluated new generation chromatography resins used in the antibody capture process including Protein A, cation exchange, and mixed mode chromatography to address the benefits and unique challenges posed by each chromatography approach. Our results demonstrate the benefit of improved binding capacity of new generation Protein A resins, address the concern of high concentration surge caused aggregation when using new generation cation exchange resins with over 100mg/mL binding capacity, and highlight the potential of multimodal cation exchange resins for capture process design. The new landscape of capture chromatography technologies provides options to achieve overall downstream purification outcome with high product quality and process efficiency. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Chelating agents as stationary phase in extraction chromatography, ch. 11

    International Nuclear Information System (INIS)

    Sebesta, F.

    1975-01-01

    Chelating agents have been used largely in extraction chromatography for separations related to activation analysis, for concentration of metals from dilute solutions, and for preparation of radiochemically pure or carrier-free radionuclides. This review deals with the theory of extraction by chelating agents, the experimental technique, and the chelating agents and systems used (β-diketones, oximes, hydroxamic acid, dithizone and diethyldithiocarbamic acid)

  8. A Size Exclusion Chromatography Laboratory with Unknowns for Introductory Students

    Science.gov (United States)

    McIntee, Edward J.; Graham, Kate J.; Colosky, Edward C.; Jakubowski, Henry V.

    2015-01-01

    Size exclusion chromatography is an important technique in the separation of biological and polymeric samples by molecular weight. While a number of laboratory experiments have been published that use this technique for the purification of large molecules, this is the first report of an experiment that focuses on purifying an unknown small…

  9. The thermodynamic principles of ligand binding in chromatography and biology

    DEFF Research Database (Denmark)

    Mollerup, Jørgen

    2007-01-01

    the general thermodynamic principles of ligand binding. Models of the multi-component adsorption in ion-exchange and hydrophobic chromatography, HIC and RPLC, are developed. The parameters in the models have a well-defined physical significance. The models are compared to the Langmuir model...

  10. Mallow carotenoids determined by high-performance liquid chromatography

    Science.gov (United States)

    Mallow (corchorus olitorius) is a green vegetable, which is widely consumed either fresh or dry by Middle East population. This study was carried out to determine the contents of major carotenoids quantitatively in mallow, by using a High Performance Liquid Chromatography (HPLC) equipped with a Bis...

  11. High-Performance Liquid Chromatography-Mass Spectrometry.

    Science.gov (United States)

    Vestal, Marvin L.

    1984-01-01

    Reviews techniques for online coupling of high-performance liquid chromatography with mass spectrometry, emphasizing those suitable for application to nonvolatile samples. Also summarizes the present status, strengths, and weaknesses of various techniques and discusses potential applications of recently developed techniques for combined liquid…

  12. Enthalpy of Vaporization by Gas Chromatography: A Physical Chemistry Experiment

    Science.gov (United States)

    Ellison, Herbert R.

    2005-01-01

    An experiment is conducted to measure the enthalpy of vaporization of volatile compounds like methylene chloride, carbon tetrachloride, and others by using gas chromatography. This physical property was measured using a very tiny quantity of sample revealing that it is possible to measure the enthalpies of two or more compounds at the same time.

  13. Use of chromatography tubes in triiodothyronine and thyroxine ria

    International Nuclear Information System (INIS)

    Inbar, D.; Tamir, Y.; Derfler, S.; Feingers, J.; Wagner, D.

    1980-01-01

    Simple, convenient radioimmunoassay for total triiodothyronine and total thyroxine in human serum are described. Ascending dry column chromatography is used to separate the free and antibody-bound hormone. The method allows for separation by absorption, without centrifugation. The use of test tubes containing pre-measured quantities of lyophilized radioactive reagent obviates unnecessary handling of radioactive solutions. (Auth.)

  14. Monitoring aged reversed-phase high performance liquid chromatography columns

    NARCIS (Netherlands)

    Bolck, A; Smilde, AK; Bruins, CHP

    1999-01-01

    In this paper, a new approach for the quality assessment of routinely used reversed-phase high performance liquid chromatography columns is presented. A used column is not directly considered deteriorated when changes in retention occur. If attention is paid to the type and magnitude of the changes,

  15. Gas Chromatography-Mass Spectrometric Analysis of Nematicidal ...

    African Journals Online (AJOL)

    Purpose: To investigate the chemical composition and nematicidal activity of the essential oil of. Valeriana amurensis ... Methods: The essential oil of V. amurensis roots was obtained by hydrodistillation and analyzed by gas chromatography (GC) ..... Aβ1-40 and Caspase-3 in Alzheimer's disease model rat's brain. J Chin ...

  16. Ultra high performance liquid chromatography of seized drugs

    NARCIS (Netherlands)

    Lurie, I.S.

    2010-01-01

    The primary goal of this thesis is to investigate the use of ultra high performance liquid chromatography (UHPLC) for the analysis of seized drugs. This goal was largely achieved and significant progress was made in achieving improved separation and detection of drugs of forensic interest.

  17. Adsorption gas chromatography with 150-ms {sup 216}Po

    Energy Technology Data Exchange (ETDEWEB)

    Vogt, A [Bern Univ. (Switzerland); Gaeggeler, H W; Tuerler, A [Paul Scherrer Inst. (PSI), Villigen (Switzerland)

    1997-09-01

    A gas chromatography apparatus was developed, which allows experiments with volatile radionuclides having shorter half-lives than one second. This apparatus was tested with the 150-ms isotope {sup 216}Po. Experimental data were compared with a Monte Carlo model to determine the adsorption enthalpy {Delta}H{sub a}. (author) 2 figs., 2 refs.

  18. Estimation of boiling point of radon by radiogas chromatography

    International Nuclear Information System (INIS)

    Takahashi, N.; Otozai, K.

    1986-01-01

    The retention volume of radon was measured by means of radiogas chromatography. The boiling point of radon was estimated from the retention volume by the use of the semi-empirical formula relating the boiling point to the retention volume. The obtained boiling point (198+-2)K was lower by 13 K than that measured by Gray and Ramsay. (author)

  19. Purification of bacteriocins using size-exclusion chromatography

    Directory of Open Access Journals (Sweden)

    Vivek K. Bajpai

    2016-06-01

    Full Text Available The bacteriocin purification involves following main steps. a. Extraction of cell-free-supernatant of bacteria. b. Ammonium sulfate precipitation. c. Dialysis. d. Diafiltration using PVP and e. Size-exclusion chromatography. However, depending on the nature of work, the compound could be further analyzed by reverse-phase HPLC, NMR, mass spectrometry and sequencing.

  20. Quantification of Tea Flavonoids by High Performance Liquid Chromatography

    Science.gov (United States)

    Freeman, Jessica D.; Niemeyer, Emily D.

    2008-01-01

    We have developed a laboratory experiment that uses high performance liquid chromatography (HPLC) to quantify flavonoid levels in a variety of commercial teas. Specifically, this experiment analyzes a group of flavonoids known as catechins, plant-derived polyphenolic compounds commonly found in many foods and beverages, including green and black…

  1. Fast temperature programming in gas chromatography using resistive heating

    NARCIS (Netherlands)

    Dallüge, J.; Ou-Aissa, R.; Vreuls, J.J.; Brinkman, U.A.T.; Veraart, J.R.

    1999-01-01

    The features of a resistive-heated capillary column for fast temperature-programmed gas chromatography (GC) have been evaluated. Experiments were carried out using a commercial available EZ Flash GC, an assembly which can be used to upgrade existing gas chromatographs. The capillary column is placed

  2. The effect of improved chromatography on GDGT-based palaeoproxies

    NARCIS (Netherlands)

    Hopmans, E.C.; Schouten, S.; Sinninghe Damsté, J.S.

    2016-01-01

    The development of methods using liquid chromatography coupled to mass spectrometry to analyze glycerol dialkyl glycerol tetraethers (GDGTs) has substantially expanded the biomarker tool box and led to the development of several new proxies. Recent studies have shown that new high performance liquid

  3. Radioactivity monitor for high-performance liquid chromatography

    International Nuclear Information System (INIS)

    Reeve, D.R.; Crozier, A.

    1977-01-01

    The coupling of a homogeneous radioactivity monitor to a liquid chromatograph involves compromises between the sensitivity of the monitor and the resolution and speed of analysis of the chromatograph. The theoretical relationships between these parameters are considered and expressions derived which make it possible to calculate suitable monitor operating conditions for most types of high-performance liquid chromatography

  4. Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography

    Czech Academy of Sciences Publication Activity Database

    Flodrová, Dana; Bobálová, Janette; Laštovičková, Markéta

    2016-01-01

    Roč. 44, č. 2 (2016), s. 286-297 ISSN 0133-3720 R&D Projects: GA ČR(CZ) GPP503/12/P395 Institutional support: RVO:68081715 Keywords : barley * wheat * glycoprotein * mass spectrometry * lectin chromatography Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 0.496, year: 2016

  5. Bayesian approach for peak detection in two-dimensional chromatography

    NARCIS (Netherlands)

    Vivó-Truyols, G.

    2012-01-01

    A new method for peak detection in two-dimensional chromatography is presented. In a first step, the method starts with a conventional one-dimensional peak detection algorithm to detect modulated peaks. In a second step, a sophisticated algorithm is constructed to decide which of the individual

  6. Characterization of porous silicon integrated in liquid chromatography chips

    NARCIS (Netherlands)

    Tiggelaar, Roald M.; Verdoold, Vincent; Eghbali, H.; Desmet, G.; Gardeniers, Johannes G.E.

    2009-01-01

    Properties of porous silicon which are relevant for use of the material as a stationary phase in liquid chromatography chips, like porosity, pore size and specific surface area, were determined with high-resolution SEM and N2 adsorption–desorption isotherms. For the anodization conditions

  7. Methods of uranium isotpic separation by chemical exchange chromatography

    International Nuclear Information System (INIS)

    Pena V, L.A.; Valle M, L.

    1985-01-01

    Chemical exchange chromatography as applied to isotope separation has undergone a constant development during the last few years. The results so far indicate that this method could eventually become commercially useful. This work presents a critical review of the experimental methods presently under study by principal research groups, and which have not get been compared. (Author)

  8. Preparative supercritical fluid chromatography: A powerful tool for chiral separations.

    Science.gov (United States)

    Speybrouck, David; Lipka, Emmanuelle

    2016-10-07

    In 2012, the 4 biggest pharmaceutical blockbusters were pure enantiomers and separating racemic mixtures is now frequently a key step in the development of a new drug. For a long time, preparative liquid chromatography was the technique of choice for the separation of chiral compounds either during the drug discovery process to get up to a hundred grams of a pure enantiomer or during the clinical trial phases needing kilograms of material. However the advent of supercritical Fluid Chromatography (SFC) in the 1990s has changed things. Indeed, the use of carbon dioxide as the mobile phase in SFC offers many advantages including high flow rate, short equilibration time as well as low solvent consumption. Despite some initial teething troubles, SFC is becoming the primary method for preparative chiral chromatography. This article will cover recent developments in preparative SFC for the separation of enantiomers, reviewing several aspects such as instrumentation, chiral stationary phases, mobile phases or purely preparative considerations including overloading, productivity or large scale chromatography. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. Supercritical fluid chromatography for lipid analysis in foodstuffs.

    Science.gov (United States)

    Donato, Paola; Inferrera, Veronica; Sciarrone, Danilo; Mondello, Luigi

    2017-01-01

    The task of lipid analysis has always challenged separation scientists, and new techniques in chromatography were often developed for the separation of lipids; however, no single technique or methodology is yet capable of affording a comprehensive screening of all lipid species and classes. This review acquaints the role of supercritical fluid chromatography within the field of lipid analysis, from the early developed capillary separations based on pure CO 2 , to the most recent techniques employing packed columns under subcritical conditions, including the niche multidimensional techniques using supercritical fluids in at least one of the separation dimensions. A short history of supercritical fluid chromatography will be introduced first, from its early popularity in the late 1980s, to the sudden fall and oblivion until the last decade, experiencing a regain of interest within the chromatographic community. Afterwards, the subject of lipid nomenclature and classification will be briefly dealt with, before discussing the main applications of supercritical fluid chromatography for food analysis, according to the specific class of lipids. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Chromatography Of Metal Ions On Wood Cellulose Impregnated ...

    African Journals Online (AJOL)

    Adsorption chromatography of some heavy metal ions on wood cellulose of saw dust (wood waste dust) modified with hydrochloric acid, urea and thiourea was studied. Atomic absorption spectrophotometry (AAS) was used to determine the initial concentration of solutions of Zn2+, Cu2+, Ni2+, Pb2+, and Fe3+ metal ions.

  11. Development of a thermal desorption modulator for gas chromatography

    NARCIS (Netherlands)

    Geus, H.J.; Boer, de J.; Brinkman, U.A.Th.

    1997-01-01

    The separation space in gas chromatography can be enhanced dramatically by the comprehensive coupling of two independent separation dimensions. An interface between the two columns must accumulate analytes eluting from the first dimension, focus them and at the appropriate moment transfer them to

  12. Csaba Horváth and preparative liquid chromatography.

    Science.gov (United States)

    Guiochon, Georges

    2005-06-24

    Few chromatographers have been interested in furthering preparative liquid chromatography. The pioneers, Tswett, Kuhn and Lederer, A.J.P. Martin, Tiselius, isolated fractions but as an intermediate step in the analysis of their samples. The progress in electronics and sensors, and in their miniaturization has lead to the paradoxical situation that the analysts never see the transient pure fractions that their detector quantitates. Yet, over the last 25 years, preparative liquid chromatography has become an important industrial process for the separation, the extraction, and/or the purification of many pharmaceuticals or pharmaceutical intermediates, including pure enantiomers, purified peptides and proteins, compounds that are manufactured at the relatively large industrial scale of a few kilograms to several hundred tons per year. This development that has strongly affected the modem pharmaceutical industry is mainly due to the pioneering work of Csaba Horváth. His work in preparative HPLC was critical at both the practical and the theoretical levels. He was the first scientist in modem times to pay serious attention to the relationships between the curvature of the equilibrium isotherms, the competitive nature of nonlinear isotherms, and the chromatographic band profiles of complex mixtures. The thermodynamics of multi-component phase equilibria and mass transfer kinetics in chromatography attracted his interest and were the focus of ground-breaking contributions. He investigated displacement chromatography, an old method invented by Tiselius that Csaba was first to implement in HPLC. This choice was explained by the essential characteristic of displacement chromatography, in that it delivers fractions that can be far more concentrated than the feed. Remarkably, once the basics of nonlinear chromatography had been mastered in his group, most of the applications that were studied by his coworkers dealt with peptides of various sizes and with proteins. Thus, all

  13. Fabrication of Ceramic Membrane Chromatography for Biologics Purification

    Directory of Open Access Journals (Sweden)

    Maizirwan Mel

    2011-12-01

    Full Text Available Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this study, a coin-like, 13 mm diameter and 3 mm thick, ceramic membrane was fabricated to be used as a chromatographic medium. The membrane is used to replace the conventional resin-based chromatography columns. Hydroxyapatite (HA powder was used as a material for the membrane fabrication. In this project, a HA powder was produced using starch as pore creating agents. Characterization processes were done for the ceramic membrane using the suitable apparatuses. Three parameters of the fabrication process (starch wt %, compaction pressure and sintering temperature were manipulated to optimize the performance of the membrane. The fabricated membrane was placed in a (FPLC system to be tested for its performance as an adsorptive membrane. (IMAC process was run by immobilizing Ni2+ ions at the membrane particles surfaces. NP protein of the (NDV was used to test the membrane's ability to bind Histidine-tagged proteins. The optimum set of process parameters that yielded in the highest porosity and good chromatogram was determined to be 5 wt % starch, 3000 psi compaction pressure and 1100°C sintering temperature.ABSTRAK: Kromatografi merupakan satu daripada proses pengasingan yang penting yang dipilih untuk perolehan/penapisan protein dan biostruktur yang kompleks. Pemfabrikatan membran kromatografi dan kecekapannya dalam proses kromatografi merupakan fokus beberapa kajian terkini. Dalam kajian ini, membran seramik berbentuk duit syiling, berdiameter 13 mm dengan ketebalan 3 mm, direka untuk digunakan sebagai perantara kromatografi. Membran ini digunakan untuk menggantikan turus kromatografi berasaskan resin yang lazim. Serbuk hidroksiapatit (HA digunakan sebagai bahan

  14. Exploration of overloaded cation exchange chromatography for monoclonal antibody purification.

    Science.gov (United States)

    Liu, Hui F; McCooey, Beth; Duarte, Tiago; Myers, Deanna E; Hudson, Terry; Amanullah, Ashraf; van Reis, Robert; Kelley, Brian D

    2011-09-28

    Cation exchange chromatography using conventional resins, having either diffusive or perfusive flow paths, operated in bind-elute mode has been commonly employed in monoclonal antibody (MAb) purification processes. In this study, the performance of diffusive and perfusive cation exchange resins (SP-Sepharose FF (SPSFF) and Poros 50HS) and a convective cation exchange membrane (Mustang S) and monolith (SO(3) Monolith) were compared. All matrices were utilized in an isocratic state under typical binding conditions with an antibody load of up to 1000 g/L of chromatographic matrix. The dynamic binding capacity of the cation exchange resins is typically below 100 g/L resin, so they were loaded beyond the point of anticipated MAb break through. All of the matrices performed similarly in that they effectively retained host cell protein and DNA during the loading and wash steps, while antibody flowed through each matrix after its dynamic binding capacity was reached. The matrices differed, though, in that conventional diffusive and perfusive chromatographic resins (SPSFF and Poros 50HS) demonstrated a higher binding capacity for high molecular weight species (HMW) than convective flow matrices (membrane and monolith); Poros 50HS displayed the highest HMW binding capacity. Further exploration of the conventional chromatographic resins in an isocratic overloaded mode demonstrated that the impurity binding capacity was well maintained on Poros 50HS, but not on SPSFF, when the operating flow rate was as high as 36 column volumes per hour. Host cell protein and HMW removal by Poros 50HS was affected by altering the loading conductivity. A higher percentage of host cell protein removal was achieved at a low conductivity of 3 mS/cm. HMW binding capacity was optimized at 5 mS/cm. Our data from runs on Poros 50HS resin also showed that leached protein A and cell culture additive such as gentamicin were able to be removed under the isocratic overloaded condition. Lastly, a MAb

  15. The Purification of a Blood Group A Glycoprotein: An Affinity Chromatography Experiment.

    Science.gov (United States)

    Estelrich, J.; Pouplana, R.

    1988-01-01

    Describes a purification process through affinity chromatography necessary to obtain specific blood group glycoproteins from erythrocytic membranes. Discusses the preparation of erythrocytic membranes, extraction of glycoprotein from membranes, affinity chromatography purification, determination of glycoproteins, and results. (CW)

  16. Determination of trimethyllead reference material using high performance liquid chromatography-inductively coupled plasma mass spectrometry

    International Nuclear Information System (INIS)

    Lu Hai; Wei Chao; Wang Jun; Chao Jingbo; Zhou Tao; Chen Dazhou

    2005-01-01

    A high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS) was combined, and the chromatography conditions were optimized. The stability and homogeneity of a trimethyllead reference material were determined using this method. (authors)

  17. Advances in silver ion chromatography for the analysis of fatty acids and triacylglycerols-2001 to 2011.

    Science.gov (United States)

    Momchilova, Svetlana M; Nikolova-Damyanova, Boryana M

    2012-01-01

    An effort is made to critically present the achievements in silver ion chromatography during the last decade. Novelties in columns, mobile-phase compositions and detectors are described. Recent applications of silver ion chromatography in the analysis of fatty acids and triacylglycerols are presented while stressing novel analytical strategies or new objects. The tendencies in the application of the method in complementary ways with reversed-phase chromatography, chiral chromatography and, especially, mass detection are outlined.

  18. VIII All-Russian symposium on molecular liquid chromatography and capillary electrophoresis. Program. Summary of reports

    International Nuclear Information System (INIS)

    2001-01-01

    Program and summary of reports of the VIII All-Russian symposium on molecular liquid chromatography and capillary electrophoresis are performed. The meeting took place 15-19 October, 2001 in Moscow. Many problems of liquid and ion exchange chromatography, capillary electrophoresis, thin-layer chromatography have been discussed extensively. Reports covering properties of sorbents and devices for chromatography are incorporated in the collection [ru

  19. Study of radiolysis products of natural organic materials by means of gas chromatography

    International Nuclear Information System (INIS)

    Pogocki, D.

    1994-01-01

    Analytical methods based on gas chromatography for identification determination of products arising during food irradiation have been presented. Behind the classics version of the methods one has shown also combined methods being the on-line connection of gas chromatography with mass spectroscopy as well as gas chromatography with liquid chromatography and mass spectroscopy. The applicability as well as weakness and advantages of each version have been discussed on the context of food irradiation. 11 refs, 7 figs

  20. 5-O-caffeoylshikimic acid from Solanum somalense leaves: advantage of centrifugal partition chromatography over conventional column chromatography.

    Science.gov (United States)

    Chideh, Saïda; Pilard, Serge; Attoumbré, Jacques; Saguez, Robert; Hassan-Abdallah, Alshaimaa; Cailleu, Dominique; Wadouachi, Anne; Baltora-Rosset, Sylvie

    2014-09-01

    Solanum somalense leaves, used in Djibouti for their medicinal properties, were extracted by MeOH. Because of the high polyphenol and flavonoid contents of the extract, respectively, determined at 80.80 ± 2.13 mg gallic acid equivalent/g dry weight and 24.4 ± 1.01 mg quercetin equivalent/g dry weight, the isolation and purification of the main polyphenols were carried out by silica gel column chromatography and centrifugal partition chromatography. Column chromatography led to 11 enriched fractions requiring further purification, while centrifugal partition chromatography allowed the easy recovery of the main compound of the extract. In a solvent system composed of CHCl3/MeOH/H2O (9.5:10:5), 21.8 mg of this compound at 97% purity was obtained leading to a yield of 2.63%. Its structure was established as 5-O-caffeoylshikimic acid by mass spectrometry and NMR spectroscopy. This work shows that S. somalense leaves contain very high level of 5-O-caffeoylshikimic acid (0.74% dry weight), making it a potential source of production of this secondary metabolite that is not commonly found in nature but could be partly responsible of the medicinal properties of S. somalense leaves. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

    International Nuclear Information System (INIS)

    Shu, Xikai; Wang, Mei; Liu, Daicheng; Wang, Daijie; Lin, Xiaojing; Liu, Jianhua; Wang, Xiao; Huang, Luqi

    2013-01-01

    An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I), 24.5 mg of luteolin-7-O-β-D-rutinoside (compound II), 18.4 mg of luteolin-7-O-β-D-glucoside (compound III), and 33.4 mg of cynarin (compound IV) with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC) method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). (author)

  2. Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Shu, Xikai; Wang, Mei; Liu, Daicheng [College of Life Science, Shandong Normal University, Jinan, Shandong (China); Wang, Daijie; Lin, Xiaojing; Liu, Jianhua; Wang, Xiao; Huang, Luqi, E-mail: wxjn1998@126.com [Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, Shandong (China)

    2013-09-01

    An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I), 24.5 mg of luteolin-7-O-{beta}-D-rutinoside (compound II), 18.4 mg of luteolin-7-O-{beta}-D-glucoside (compound III), and 33.4 mg of cynarin (compound IV) with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC) method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). (author)

  3. Electron-capture process and ion mobility spectra in plasma chromatography

    International Nuclear Information System (INIS)

    Karasek, F.W.; Spangler, G.E.

    1981-01-01

    The basic principles of plasma chromatography are introduced and ion mobility relationships presented. The relationships of plasma chromatography to electron-capture detector mechanisms are discussed, including electron energy considerations and electron-capture reactions. A number of experimental studies by plasma chromatography are described. (C.F.)

  4. Normal and Reversed-Phase Thin Layer Chromatography of Green Leaf Extracts

    Science.gov (United States)

    Sjursnes, Birte Johanne; Kvittingen, Lise; Schmid, Rudolf

    2015-01-01

    Introductory experiments of chromatography are often conducted by separating colored samples, such as inks, dyes, and plant extracts, using filter paper, chalk, or thin layer chromatography (TLC) plates with various solvent systems. Many simple experiments have been reported. The relationship between normal chromatography and reversed-phase…

  5. Theories to support method development in comprehensive two-dimensional liquid chromatography - A review

    NARCIS (Netherlands)

    Bedani, F.; Schoenmakers, P.J.; Janssen, H.-G.

    2012-01-01

    On-line comprehensive two-dimensional liquid chromatography techniques promise to resolve samples that current one-dimensional liquid chromatography methods cannot adequately deal with. To make full use of the potential of two-dimensional liquid chromatography, optimization is required. Optimization

  6. 21 CFR 862.2250 - Gas liquid chromatography system for clinical use.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Gas liquid chromatography system for clinical use... Instruments § 862.2250 Gas liquid chromatography system for clinical use. (a) Identification. A gas liquid chromatography system for clinical use is a device intended to separate one or more drugs or compounds from a...

  7. 21 CFR 862.2260 - High pressure liquid chromatography system for clinical use.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false High pressure liquid chromatography system for... Clinical Laboratory Instruments § 862.2260 High pressure liquid chromatography system for clinical use. (a) Identification. A high pressure liquid chromatography system for clinical use is a device intended to separate...

  8. Electrochemically modulated liquid chromatography: Theoretical investigations and applications from the perspectives of chromatography and interfacial electrochemistry

    Energy Technology Data Exchange (ETDEWEB)

    Keller, David W. [Iowa State Univ., Ames, IA (United States)

    2005-01-01

    Electrochemically modulated liquid chromatography (EMLC) employs a conductive material as both a stationary phase for chromatographic separations and as a working electrode for performing electrochemistry experiments. This dual functionality gives EMLC the capacity to manipulate chromatographic separations by changing the potential applied (Eapp) to the stationary phase with respect to an external reference. The ability to monitor retention as a function of Eapp provides a means to chromatographically monitor electrosorption processes at solid-liquid interfaces. In this dissertation, the retention mechanism for EMLC is examined from the perspective of electrical double layer theory and interfacial thermodynamics. From the chromatographic data, it is possible to determine the interfacial excess (Λ) of a solute and changes in interfacial tension (dγ) as a function of both Eapp and the supporting electrolyte concentration. Taken together, these two experimentally manipulated parameters can be examined within the context of the Gibbs adsorption equation to delineate the contribution of a variety of interfacial properties, including the charge of solute on the stationary phase and the potential of zero charge (PZC), to the mechanism behind EMLC-based retention. The chromatographic probing of interfacial phenomena is complemented by electroanalytical experiments that exploit the ability to monitor the electronic current flowing through an EMLC column. Cyclic voltammetry and chronoamperometry of an EMLC column are used to determine the electronic performance characteristics of an EMLC column. An electrochemical flow injection analysis of a column is provided in which the current required to maintain a constant Eapp is monitored and provides a way to examine the influence that acetonitrile and supporting electrolyte composition, flow rate, column backpressure, and ionic strength have on the structure of electrified interfaces.

  9. Arzneimittelentwicklung zu vernachlässigten Krankheiten

    Directory of Open Access Journals (Sweden)

    Katrin Gerlinger

    2017-08-01

    Full Text Available Krankheiten, die fast ausschließlich in armen Ländern auftreten, werden seit Jahrzehnten in der medizinischen Forschung und Entwicklung vernachlässigt. Eine Ursache wird in der zahlungsschwachen Nachfrage gesehen. Sie kann die aufwendige kommerzialisierte Produktentwicklung nicht in ausreichendem Maße anreizen. Seit etlichen Jahren werden Wege gesucht, das systemische Problem des patentbasierten Innovationsprozesses zumindest bei armutsassoziierten Krankheiten abzumildern. Einige Akteure testen, inwiefern sich eine Grundidee der Open-Source-Softwareentwicklung – die Zusammenarbeit Freiwilliger bringt Produkte hervor, die keiner Firma allein gehören – auf die Arzneimittelentwicklung übertragen lässt. Dafür öffnen sie unterschiedliche Elemente des pharmakologischen Innovationsprozesses für eine gemeinsame Nutzung und Weiterentwicklung. Sie bezeichnen dies teilweise als Open Innovation. Der Artikel stellt relevante Initiativen vor.

  10. Elektrochemischer Sensor und Verfahren zu seiner Herstellung

    OpenAIRE

    Vonau, W.; Kaden, H.; Kretzschmar, C.; Otschik, P.; Krabbes, I.; Woithe, W.; Grosse, M.

    2002-01-01

    Electro-chemical sensor for the detection of ion activity comprises a selective glas membrane (4) in direct contact with a metal electrode (3) or in direct contact with a variable conducting glas layer (4a), which is itself in contact with (3). (3), (4) and (4a) are plated onto a steel-ceramic substrate using screen-print technology. The thermal expansion coefficients of the steel-ceramic substrate and the glas layers differ by a maximum of = 6 multiply 10-7 K-1. USE - The sensor is especiall...

  11. Riots ‒ Anmerkungen zu Begriff und Konzept

    Directory of Open Access Journals (Sweden)

    Dieter Rucht

    2016-05-01

    Full Text Available Ausgehend von sozialwissenschaftlichen Befunden werden zunächst ältere massenpsychologische Deutungen von riots zurückgewiesen. Die überwiegende Mehrzahl der im riot Aufbegehrenden entspricht nicht dem Typus entfremdeter und verängstigter Einzeltäter_innen. Zweitens benennt der Beitrag eine Reihe von Bedingungen für das Zustandekommen von riots. Drittens wird der Begriff ‚riot‘ in formaler Hinsicht näher bestimmt und einerseits von moderateren Formen des Protests, andererseits von geplanter und organisierter Gewalt (z. B. Terrorismus abgegrenzt. Abschließend wird die Frage des politischen Gehalts von riots und deren normativen Grundlagen erörtert.

  12. Tropfenschlagerosionsgeschuetzte Turbinenschaufel und Verfahren zu ihrer Herstellung

    OpenAIRE

    Brenner, B.; Storch, W.; Wiedemann, G.

    1995-01-01

    The invention refers to the surface hardening of machine components. The objective of the invention of providing and manufacturing turbine blades with better protection against droplet impact erosion is solved according to the invention et al. by the fact that the constant surface hardness of a very hard layer (2) of the turbine blade represented in the drawing corresponds to the maximum hardness of the steel selected, the depth of this very hard layer (2) is 0.1 to 0.9 mm, the total hardenin...

  13. Liquid chromatography and supercritical fluid chromatography as alternative techniques to gas chromatography for the rapid screening of anabolic agents in urine.

    Science.gov (United States)

    Desfontaine, Vincent; Nováková, Lucie; Ponzetto, Federico; Nicoli, Raul; Saugy, Martial; Veuthey, Jean-Luc; Guillarme, Davy

    2016-06-17

    This work describes the development of two methods involving supported liquid extraction (SLE) sample treatment followed by ultra-high performance liquid chromatography or ultra-high performance supercritical fluid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS and UHPSFC-MS/MS) for the screening of 43 anabolic agents in human urine. After evaluating different stationary phases, a polar-embedded C18 and a diol columns were selected for UHPLC-MS/MS and UHPSFC-MS/MS, respectively. Sample preparation, mobile phases and MS conditions were also finely tuned to achieve highest selectivity, chromatographic resolution and sensitivity. Then, the performance of these two methods was compared to the reference routine procedure for steroid analyses in anti-doping laboratories, which combines liquid-liquid extraction (LLE) followed by gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). For this purpose, urine samples spiked with the compounds of interest at five different concentrations were analyzed using the three analytical platforms. The retention and selectivity of the three techniques were very different, ensuring a good complementarity. However, the two new methods displayed numerous advantages. The overall procedure was much faster thanks to high throughput SLE sample treatment using 48-well plates and faster chromatographic analysis. Moreover, the highest sensitivity was attained using UHPLC-MS/MS with 98% of the doping agents detected at the lowest concentration level (0.1ng/mL), against 76% for UHPSFC-MS/MS and only 14% for GC-MS/MS. Finally, the weakest matrix effects were obtained with UHPSFC-MS/MS with 76% of the analytes displaying relative matrix effect between -20 and 20%, while the GC-MS/MS reference method displayed very strong matrix effects (over 100%) for all of the anabolic agents. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Threshold-Konzepte, das ANCIL-Curriculum und die Metaliteracy – Überlegungen zu Konsequenzen für die Förderung von Informationskompetenz in deutschen Hochschulen

    Directory of Open Access Journals (Sweden)

    Wilfried Sühl-Strohmenger

    2017-04-01

    Full Text Available Das Verständnis von Informationskompetenz, wie es sich im deutschsprachigen Raum seit etwa 15 Jahren entwickelt hat, steht auch aufgrund neuer Ansätze und Ideen zur Information Literacy aus dem angloamerikanischen Raum zur Diskussion. Vor allem bezieht sich das auf die Threshold-Konzepte sowie das Framework-Modell der US-amerikanischen Association of College & Research Libraries (ACRL, ferner die Metaliteracy-Theorie von Mackey und Jacobson und schließlich auf das neue britische ANCIL-Curriculum. Nun sind aus deutscher bibliothekarischer Sicht nicht alle mit den oben genannten neuen Konzepten verbundenen Gedankengänge und Argumente der Kolleginnen und Kollegen aus den USA und aus Großbritannien ohne weiteres nachvollziehbar gewesen. Und wie verhalten sie sich zu den Standards der Informationskompetenz? Jetzt liegen erste Ansätze in den USA vor, die explizit auf dem Threshold-Modell bzw. dem ACRL-Framework aufbauen, so dass eine konkretere Grundlage für mögliche Übertragungen der Modelle auf Kurs- und Schulungskonzepte zur Förderung von Informationskompetenz im deutschsprachigen Raum gegeben ist.   The concept of information competency, as developed in the German-speaking countries for about 15 years, has recently come up for discussion because of new approaches and ideas about information literacy from the Anglo-American countries. This mostly refers to the threshold concepts as well as the framework model of the US-American ACRL, the Metaliteracy Theory by Mackey and Jacobson and finally the new curriculum of the British ANCIL. From a German librarian's point of view, not all of the ideas and arguments of our American and British colleagues which are connected to the above-mentioned new concepts were easy to understand. Another question is how they relate to the standards of information competency. By now, first approaches in the US, which are explicitly based on the Threshold Model or the ACRL Framework, have been made available

  15. Durch Kooperation und Vernetzung zu neuen Aufgabenfeldern – Serviceentwicklung in der Bibliothek der Medizinischen Fakultät Mannheim [Opening up new tasks through cooperation and networking – service development in the Library for the Medical Faculty of Mannheim

    Directory of Open Access Journals (Sweden)

    Semmler-Schmetz, Martina

    2013-09-01

    - und Beratungsangebote entwickelt werden. Die jüngste Kooperation mit Akteuren auf dem Gebiet der evidenzbasierten Medizin hat einen innovativen Service „Systematische Literaturrecherche“ hervorgebracht, der Ärzte und Wissenschaftler bei der Erstellung von Förderanträgen zu klinischen Studien sowie systematischen Übersichtsarbeiten und Leitlinien unterstützt. Mit entsprechenden kostenpflichtigen Services testet die Bibliothek nun auch eine Öffnung ihrer neuen Angebote für Ärzte, Wissenschaftler und Angehörige von Gesundheitsberufen in der Region.

  16. Normalization of natural gas composition data measured by gas chromatography

    International Nuclear Information System (INIS)

    Milton, Martin J T; Harris, Peter M; Brown, Andrew S; Cowper, Chris J

    2009-01-01

    The composition of natural gas determined by gas chromatography is routinely used as the basis for calculating physico-chemical properties of the gas. Since the data measured by gas chromatography have particular statistical properties, the methods used to determine the composition can make use of a priori assumptions about the statistical model for the data. We discuss a generalized approach to determining the composition, and show that there are particular statistical models for the data for which the generalized approach reduces to the widely used method of post-normalization. We also show that the post-normalization approach provides reasonable estimates of the composition for cases where it cannot be shown to arise rigorously from the statistical structure of the data

  17. Nano-liquid chromatography applied to enantiomers separation.

    Science.gov (United States)

    Fanali, Salvatore

    2017-02-24

    This paper presents the state of the art concerning the separation of chiral compounds by means of nano-liquid chromatography (nano-LC). The enantiomers' separation and determination are a subject of fundamental importance in various application fields such as pharmaceutical industry, biomedicine, food, agrochemical etc. Nano-LC is a miniaturized chromatographic technique offering some advantages over conventional ones such as low consumption of mobile phase, sample volume and amount of chiral stationary phase, reduced costs etc. This is reported in the first part of the paper illustrating the features of the nano-LC. In addition, chiral resolution methods are briefly illustrated. Some chiral selectors, used in high-performance liquid chromatography have also been applied in nano-LC including cyclodextrins, glycopeptide antibiotics, modified polysaccharides etc. This is discussed in the second part of the review. Finally some examples of the applications available in literature are reported. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Modeling of Hydrodynamic Chromatography for Colloid Migration in Fractured Rock

    International Nuclear Information System (INIS)

    Li Shihhai; Jen, C.-P.

    2001-01-01

    The role of colloids in the migration of radionuclides in the geosphere has been emphasized in the performance assessment of high-level radioactive waste disposal. The literature indicates that the colloid velocity may not be equal to the velocity of groundwater owing to hydrodynamic chromatography. A theoretical model for hydrodynamic chromatography of colloid migration in the fracture is proposed in the present work. In this model, the colloids are treated as nonreactive and the external forces acting on colloidal particles are considered including the inertial force, the van der Waals attractive force, and the electrical double-layer repulsive force, as well as the gravitational force. A fully developed concentration profile for colloids is obtained to elucidate migration behavior for colloids in the fracture. The effects of parameters governing these forces and the aperture of the fracture are determined using a theoretical model

  19. Fractionation of lithium isotopes in cation-exchange chromatography

    International Nuclear Information System (INIS)

    Oi, Takao; Kawada, Kazuhiko; Kakihana, Hidetake; Hosoe, Morikazu

    1991-01-01

    Various methods for lithium isotope separation have been developed, and their applicability to large-scale enriched lithium isotope production has been assessed. Ion-exchange chromatography is one such method. Cation-exchange chromatography of lithium was carried out to investigate the lithium isotope effect in aqueous ion-exchange systems. The heavier isotope. 7 Li, was preferentially fractionated into the resin phase in every experiment conducted, and this result is consistent with the results of previous work. The value of the separation factor was 1.00089-1.00171 at 25C. A comparison of lithium isotope effect with those of potassium and rubidium indicated that the isotope effect originating from hydration is larger than the effect due to phase change for lithium, while the opposite is the case with potassium and rubidium

  20. Specific capture of uranyl protein targets by metal affinity chromatography

    International Nuclear Information System (INIS)

    Basset, C.; Dedieu, A.; Guerin, P.; Quemeneur, E.; Meyer, D.; Vidaud, C.

    2008-01-01

    To improve general understanding of biochemical mechanisms in the field of uranium toxicology, the identification of protein targets needs to be intensified. Immobilized metal affinity chromatography (IMAC) has been widely developed as a powerful tool for capturing metal binding proteins from biological extracts. However uranyl cations (UO 2 2+ ) have particular physico-chemical characteristics which prevent them from being immobilized on classical metal chelating supports. We report here on the first development of an immobilized uranyl affinity chromatography method, based on the cation-exchange properties of amino-phosphonate groups for uranyl binding. The cation distribution coefficient and loading capacity on the support were determined. Then the stability of the uranyl-bonded phase under our chromatographic conditions was optimized to promote affinity mechanisms. The successful enrichment of uranyl binding proteins from human serum was then proven using proteomic and mass spectral analysis. (authors)

  1. Enrichment and Determination of radionuclides by ion chromatography

    International Nuclear Information System (INIS)

    ZAFIMANJATO, J.L.R.

    1996-01-01

    The fundamentals of Ion Chromatography (IC) and Liquid Scintillation Counting (LSC) are reviewed. Ion Chromatography as separation method for cations is coupled with Liquid Scintillation Counting for the determination of Radionuclides in water samples. An experimental arrangement for investigations on the applicability of guard columns for cationic radionuclide enrichment is shown. The saturation behaviour of single and bivalentic cations and their combination is presented. Our results show that radioactive bivalentic cations like strontium-90 and radium-226 are enriched on a Ion Pac CG 12 Dionex guard column from 100 to 300ml natural water in one single step. The procedure is suitable for their determination in concentrations down to 10 -2 Bq.l -1 [fr

  2. Determination of dibutylphosphate and monobutylphosphate in TBP by ion chromatography

    International Nuclear Information System (INIS)

    Siva Kumar, B.; Vijayalakshmi, S.; Sankaran, K.; Ganesan, V.

    2012-01-01

    Tri-n-butyl phosphate (TBP) is used as solvent in the PUREX (Plutonium Uranium Refining by Extraction) process of nuclear fuel reprocessing. TBP undergoes chemical and radiological degradation to give DBP and MBP which in turn extracts the heavy metal such as U, Pu thereby affecting the performance of the extraction process. Analytical method using ion chromatography (IC) was developed for the determination of dibutyl phosphate (DBP) and monobutyl phosphate (MBP) in TBP. In this method, DBP and MBP were extracted from tri-n-butyl phosphate using carbonate-bicarbonate mixture of eluent composition and the aqueous phase was analyzed using suppressed ion chromatography employing carbonate as eluent. Standardization of extraction was carried out by standard addition studies. The detection limits for both DBP and MBP are found to be in sub ppm level. This method was applied to the analysis of TBP supplied by different suppliers

  3. Forensic applications of supercritical fluid chromatography - mass spectrometry.

    Science.gov (United States)

    Pauk, Volodymyr; Lemr, Karel

    2018-06-01

    Achievements of supercritical fluid chromatography with mass spectrometric detection made in the field of forensic science during the last decade are reviewed. The main topics include analysis of traditional drugs of abuse (e.g. cannabis, methamphetamine) as well as new psychoactive substances (synthetic cannabinoids, cathinones and phenethylamines), doping agents (anabolic steroids, stimulants, diuretics, analgesics etc.) and chemical warfare agents. Control of food authenticity, detection of adulteration and identification of toxic substances in food are also pointed out. Main aspects of an analytical workflow, such as sample preparation, separation and detection are discussed. A special attention is paid to the performance characteristics and validation parameters of supercritical fluid chromatography-mass spectrometric methods in comparison with other separation techniques. Copyright © 2018 Elsevier B.V. All rights reserved.

  4. Approaches for on-line coupling of extraction and chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Hyoetylaeinen, Tuulia; Riekkola, Marja-Liisa [Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, P.O. Box 55, 00014, Helsinki (Finland)

    2004-04-01

    This review provides an overview of the approaches available in order to perform on-line coupling of various extraction techniques with liquid and gas chromatography, for the analysis of semivolatile and nonvolatile analytes in liquid and solid samples. The main focus is on the instrumental set-up of these techniques. Selected real applications are described by way of illustration. The extraction methods suitable for on-line coupling covered in this review are: liquid-liquid extraction, solid-phase extraction, membrane-based techniques, pressurised liquid extraction, supercritical fluid extraction, and microwave- and sonication-assisted extractions. The following systems are not covered in this review: on-line coupled solid-phase extraction-liquid chromatography, purge-and-trap-GC, and membrane extraction with a sorbent interface-GC. (orig.)

  5. Toward ideal separation by size-exclusion chromatography

    Czech Academy of Sciences Publication Activity Database

    Netopilík, Miloš

    2017-01-01

    Roč. 1487, 3 March (2017), s. 139-146 ISSN 0021-9673 R&D Projects: GA ČR(CZ) GC17-04258J Grant - others:OPPK(XE) CZ.2.16/3.1.00/24504 Institutional support: RVO:61389013 Keywords : size-exclusion chromatography * separation mechanism * band broadening function Subject RIV: CD - Macromolecular Chemistry OBOR OECD: Polymer science Impact factor: 3.981, year: 2016

  6. Application of gas chromatography to the investigations on polypropylene radiolysis

    International Nuclear Information System (INIS)

    Zagorski, Z.P.; Gluszewski, W.

    2006-01-01

    Refinement of the gas chromatography (GC) instrumental approach permitted not only improvement of investigation in basic research, but also development of a new kind of polypropylene blends, more suitable for the production of medical devices and radiation sterilization. It has been shown, that using the GC method not only methane and carbon dioxide can be measured, but also the consumption of oxygen which reacts with free radicals on the polypropylene chain

  7. Combined high-performance liquid chromatography-radioimmunoassay for cytokinins

    International Nuclear Information System (INIS)

    MacDonald, E.M.S.; Akiyoshi, D.E.; Morris, R.O.

    1981-01-01

    The cytokinins isopentenyladenosine and ribosylzeatin were conjugated to bovine serum albumin and the conjugates used to raise antisera in rabbits. The resulting antisera had high specificity towards the cytokinin haptens and low cross-reactivity towards other purines. They were used as the basis for a radioimmunoassay for cytokinins, which, when applied in conjunction with high-performance liquid chromatography, allowed rapid and sensitive (to the picogram range) estimation and identification of multiple cytokinins from natural plant and bacterial sources. (orig.)

  8. High performance liquid chromatography in studies of radiolabeled antibodies

    International Nuclear Information System (INIS)

    Hnatowich, D.J.

    1986-01-01

    High performance liquid chromatography (HPLC) as applied to the separation of antibodies displays the same advantages as in its other applications, namely good resolution accompanied by fast analysis. It is therefore not surprising that many HPLC columns designed for use with antibodies and other proteins are now available commercially. The properties of proteins which provide the separation are size, hydrophobicity, charge and affinity. The features of each are discussed. (author)

  9. Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

    OpenAIRE

    Fekete, Szabolcs; Grand-Guillaume-Perrenoud, Alexandre; Guillarme, Davy

    2014-01-01

    The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received ...

  10. Size exclusion chromatography models and its comparison with experiment

    Czech Academy of Sciences Publication Activity Database

    Netopilík, Miloš

    2017-01-01

    Roč. 8, 4 (Suppl) (2017), s. 29 E-ISSN 2157-7064. [International Conference and Exhibition on Advances in Chromatography & HPLC Techniques /3./. 13.07.2017-14.07.2017, Berlin] R&D Projects: GA ČR(CZ) GC17-04258J Institutional support: RVO:61389013 Keywords : model of separation * flow-rate influence Subject RIV: CD - Macromolecular Chemistry

  11. Isolation of Methoxyfuranocoumarins From Ammi majus by Centrifugal Partition Chromatography.

    Science.gov (United States)

    Bartnik, Magdalena; Mazurek, Anna Katarzyna

    2016-01-01

    Pure methoxyfuranocoumarins were isolated from Ammi majus L. by use of low-pressure column chromatography (LPCC) followed by centrifugal partition chromatography (CPC). The concentrated petroleum ether extract from fruits of A. majus was fractionated on a silica gel column using a gradient of ethyl acetate in dichloromethane (0-80%, v/v). Coumarin-rich fractions were analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography with diode array detection (HPLC/DAD). Xanthotoxin (8-MOP) and isopimpinellin (isoP), structurally similar compounds, were isolated in one fraction (FR6). To avoid multistep and long-lasting TLC preparation, optimization of CPC conditions has been performed. In one run, an effective separation of 8-MOP and isoP was achieved. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (10 : 8 : 10 : 9; v/v) in an ascending mode (the aqueous phase was a stationary phase, and the organic phase was a mobile phase), with flow rate 3 mL/min and rotation speed 1,600 r.p.m., was used. The identification and high purities of isolated 8-MOP (98.7%) and isoP (100%) were confirmed by HPLC/DAD assay, when compared with standards. The developed CPC method could be applied to the effective isolation of 8-MOP and isoP from plant extracts. The high purity of obtained compounds makes possible further exploitation of these components in biological studies. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  12. Chromatography, solid-phase extraction, and capillary electrochromatography with MIPs.

    Science.gov (United States)

    Tóth, Blanka; Horvai, George

    2012-01-01

    Most analytical applications of molecularly imprinted polymers are based on their selective adsorption properties towards the template or its analogs. In chromatography, solid phase extraction and electrochromatography this adsorption is a dynamic process. The dynamic process combined with the nonlinear adsorption isotherm of the polymers and other factors results in complications which have limited the success of imprinted polymers. This chapter explains these problems and shows many examples of successful applications overcoming or avoiding the problems.

  13. Application of ion chromatography in pharmaceutical and drug analysis.

    Science.gov (United States)

    Jenke, Dennis

    2011-08-01

    Ion chromatography (IC) has developed and matured into an important analytical methodology in a number of diverse applications and industries, including pharmaceuticals. This manuscript provides a review of IC applications for the determinations of active and inactive ingredients, excipients, degradation products, and impurities relevant to pharmaceutical analyses and thus serves as a resource for investigators looking for insights into the use of the IC methodology in this field of application.

  14. Nanomaterials as stationary phases and supports in liquid chromatography.

    Science.gov (United States)

    Beeram, Sandya R; Rodriguez, Elliott; Doddavenkatanna, Suresh; Li, Zhao; Pekarek, Allegra; Peev, Darin; Goerl, Kathryn; Trovato, Gianfranco; Hofmann, Tino; Hage, David S

    2017-10-01

    The development of various nanomaterials over the last few decades has led to many applications for these materials in liquid chromatography (LC). This review will look at the types of nanomaterials that have been incorporated into LC systems and the applications that have been explored for such systems. A number of carbon-based nanomaterials and inorganic nanomaterials have been considered for use in LC, ranging from carbon nanotubes, fullerenes and nanodiamonds to metal nanoparticles and nanostructures based on silica, alumina, zirconia and titanium dioxide. Many ways have been described for incorporating these nanomaterials into LC systems. These methods have included covalent immobilization, adsorption, entrapment, and the synthesis or direct development of nanomaterials as part of a chromatographic support. Nanomaterials have been used in many types of LC. These applications have included the reversed-phase, normal-phase, ion-exchange, and affinity modes of LC, as well as related methods such as chiral separations, ion-pair chromatography and hydrophilic interaction liquid chromatography. Both small and large analytes (e.g., dyes, drugs, amino acids, peptides and proteins) have been used to evaluate possible applications for these nanomaterial-based methods. The use of nanomaterials in columns, capillaries and planar chromatography has been considered as part of these efforts. Potential advantages of nanomaterials in these applications have included their good chemical and physical stabilities, the variety of interactions many nanomaterials can have with analytes, and their unique retention properties in some separation formats. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Liquid Chromatography for Analysis of Metformin in Myrmeleon sp.

    OpenAIRE

    Afidatul Muadifah; Hermin Sulistyarti; Sasangka Prasetyawan

    2017-01-01

    Myrmeleon sp is a typical of insect larva which has been used in Indonesia for diabetes treatment. However, there is no sufficient scientific report explaining the bioactive compounds in this insect. Based on our preliminary research, this insect contained metformin, i.e. one of bioactive compounds for the treatment of type-2 diabetes. Therefore, this study is focused on the development of separation technique using high performance liquid chromatography (HPLC) on a reverse phase C-18 column ...

  16. MEMS-Based Micro Gas Chromatography: Design, Fabrication and Characterization

    OpenAIRE

    Zareian-Jahromi, Mohammad Amin

    2009-01-01

    This work is focused on the design, fabrication and characterization of high performance MEMS-based micro gas chromatography columns having wide range of applications in the pharmaceutical industry, environmental monitoring, petroleum distillation, clinical chemistry, and food processing. The first part of this work describes different approaches to achieve high-performance microfabricated silicon-glass separation columns for micro gas chromatographic (µGC) systems. The capillary width effec...

  17. Radioanalytical determination of plutonium and americium using ion exchange and extraction chromatography technique in urine

    International Nuclear Information System (INIS)

    Santhanakrishnan, V.; Sreedevi, K.R.; Rajaram, S.; Ravi, P.M.

    2011-01-01

    The use of anion exchange chromatography for the separation of Pu and extraction chromatography technique for the separation of Am from urine samples was studied. In the earlier method, Pu separation was carried out by anion exchange chromatography followed by Am separation by cation exchange chromatography. The chemical recovery of Am obtained by cation exchange separation method was inconsistent and low in the range 30-70%. In this study, the average Pu recovery obtained using anion exchange chromatography was 89.2 with standard deviation of 10.4 and the average Am recovery obtained using extraction chromatography with TRU resin was 77.4 with standard deviation of 14.8. Moreover, Am separation could be completed within three hours using the TRU column compared to two days that were required for the cation exchange chromatography. (author)

  18. Expanded-bed chromatography in primary protein purification.

    Science.gov (United States)

    Anspach, F B; Curbelo, D; Hartmann, R; Garke, G; Deckwer, W D

    1999-12-31

    Chromatography in stable expanded beds enables proteins to be recovered directly from cultivations of microorganisms or cells and preparations of disrupted cells, without the need for prior removal of suspended solids. The general performance of an expanded bed is comparable to a packed bed owing to reduced mixing of the adsorbent particles in the column. However, optimal operating conditions are more restricted than in a packed bed due to the dependence of bed expansion on the size and density of the adsorbent particles as well as the viscosity and density of the feedstock. The feedstock composition may become the most limiting restriction owing to interactions of adsorbent particles with cell surfaces, DNA and other substances, leading to their aggregation and consequently to bed instabilities and channeling. Despite these difficulties, expanded-bed chromatography has found widespread applications in the large scale purification of proteins from mammalian cell and microbial feedstocks in industrial bioprocessing. The basics and implementation of expanded-bed chromatography, its advantages as well as problems encountered in the use of this technique for the direct extraction of proteins from unclarified feedstocks are addressed.

  19. Ion-pair chromatography of nucleic acid derivatives

    International Nuclear Information System (INIS)

    Perrone, P.A.; Brown, P.R.

    1985-01-01

    Little work has been done on the ion-pair chromatography of nucleic acid constituents, although there is a great potential for the use of this technique in the field. Since the classic work in 1949, nucleotides, as well as nucleosides and bases, have been separated by ion-exchange chromatography. However, ion exchange is a difficult mode and most researchers prefer the use of reversed-phase whenever possible. Although reversed-phase is now the method of choice, ionic compounds like nucleotides and some of the more polar bases are not adequately retained by many systems of this type. In addition, it is difficult to analyze simultaneously members of all three classes of nucleic acid compounds (bases, nucleosides, and nucleotides) using a reversed-phase system, even with gradient elution. Ion pairing can be a useful technique because, theoretically, the separation of nonionic bases and nucleosides along with the ionic nucleotides can be achieved. Additionally, each group of compounds may be separated isocratically. In this chapter, they will discuss ion-pair chromatography as applied to nucleic acid constituents. The current theories, advantages and disadvantages, a limited number of applications, and potential for future work are presented

  20. Affinity chromatography: A versatile technique for antibody purification.

    Science.gov (United States)

    Arora, Sushrut; Saxena, Vikas; Ayyar, B Vijayalakshmi

    2017-03-01

    Antibodies continue to be extremely utilized entities in myriad applications including basic research, imaging, targeted delivery, chromatography, diagnostics, and therapeutics. At production stage, antibodies are generally present in complex matrices and most of their intended applications necessitate purification. Antibody purification has always been a major bottleneck in downstream processing of antibodies, due to the need of high quality products and associated high costs. Over the years, extensive research has focused on finding better purification methodologies to overcome this holdup. Among a plethora of different techniques, affinity chromatography is one of the most selective, rapid and easy method for antibody purification. This review aims to provide a detailed overview on affinity chromatography and the components involved in purification. An array of support matrices along with various classes of affinity ligands detailing their underlying working principles, together with the advantages and limitations of each system in purifying different types of antibodies, accompanying recent developments and important practical methodological considerations to optimize purification procedure are discussed. Copyright © 2016 Elsevier Inc. All rights reserved.

  1. Principles of Micellar Electrokinetic Capillary Chromatography Applied in Pharmaceutical Analysis

    Directory of Open Access Journals (Sweden)

    Árpád Gyéresi

    2013-02-01

    Full Text Available Since its introduction capillary electrophoresis has shown great potential in areas where electrophoretic techniques have rarely been used before, including here the analysis of pharmaceutical substances. The large majority of pharmaceutical substances are neutral from electrophoretic point of view, consequently separations by the classic capillary zone electrophoresis; where separation is based on the differences between the own electrophoretic mobilities of the analytes; are hard to achieve. Micellar electrokinetic capillary chromatography, a hybrid method that combines chromatographic and electrophoretic separation principles, extends the applicability of capillary electrophoretic methods to neutral analytes. In micellar electrokinetic capillary chromatography, surfactants are added to the buffer solution in concentration above their critical micellar concentrations, consequently micelles are formed; micelles that undergo electrophoretic migration like any other charged particle. The separation is based on the differential partitioning of an analyte between the two-phase system: the mobile aqueous phase and micellar pseudostationary phase. The present paper aims to summarize the basic aspects regarding separation principles and practical applications of micellar electrokinetic capillary chromatography, with particular attention to those relevant in pharmaceutical analysis.

  2. The isotope separation by ion exchange chromatography. Application to the lithium isotopes separation; La separation isotopique par chromatographie ionique

    Energy Technology Data Exchange (ETDEWEB)

    Albert, M G; Barre, Y; Neige, R

    1994-12-31

    In this work is described the used study step to demonstrate the industrial feasibility of a lithium isotopes separation process by ion exchange chromatography. After having recalled how is carried out the exchange reaction between the lithium isotopes bound on the cations exchanger resin and those which are in solution and gave the ion exchange chromatography principle, the authors establish a model which takes into account the cascade theory already used for enriched uranium production. The size parameters of this model are: the isotopic separation factor (which depends for lithium of the ligands nature and of the coordination factor), the isotopic exchange kinetics and the mass flow (which depends of the temperature, the lithium concentration, the resins diameter and the front advance). The way they have to be optimized and the implementation of the industrial process are given. (O.M.). 5 refs.

  3. Bacterial Cell Wall Precursor Phosphatase Assays Using Thin-layer Chromatography (TLC) and High Pressure Liquid Chromatography (HPLC).

    Science.gov (United States)

    Pazos, Manuel; Otten, Christian; Vollmer, Waldemar

    2018-03-20

    Peptidoglycan encases the bacterial cytoplasmic membrane to protect the cell from lysis due to the turgor. The final steps of peptidoglycan synthesis require a membrane-anchored substrate called lipid II, in which the peptidoglycan subunit is linked to the carrier lipid undecaprenol via a pyrophosphate moiety. Lipid II is the target of glycopeptide antibiotics and several antimicrobial peptides, and is degraded by 'attacking' enzymes involved in bacterial competition to induce lysis. Here we describe two protocols using thin-layer chromatography (TLC) and high pressure liquid chromatography (HPLC), respectively, to assay the digestion of lipid II by phosphatases such as Colicin M or the LXG toxin protein TelC from Streptococcus intermedius . The TLC method can also monitor the digestion of undecaprenyl (pyro)phosphate, whereas the HPLC method allows to separate the di-, mono- or unphosphorylated disaccharide pentapeptide products of lipid II.

  4. Lipidomic analysis of biological samples: Comparison of liquid chromatography, supercritical fluid chromatography and direct infusion mass spectrometry methods.

    Science.gov (United States)

    Lísa, Miroslav; Cífková, Eva; Khalikova, Maria; Ovčačíková, Magdaléna; Holčapek, Michal

    2017-11-24

    Lipidomic analysis of biological samples in a clinical research represents challenging task for analytical methods given by the large number of samples and their extreme complexity. In this work, we compare direct infusion (DI) and chromatography - mass spectrometry (MS) lipidomic approaches represented by three analytical methods in terms of comprehensiveness, sample throughput, and validation results for the lipidomic analysis of biological samples represented by tumor tissue, surrounding normal tissue, plasma, and erythrocytes of kidney cancer patients. Methods are compared in one laboratory using the identical analytical protocol to ensure comparable conditions. Ultrahigh-performance liquid chromatography/MS (UHPLC/MS) method in hydrophilic interaction liquid chromatography mode and DI-MS method are used for this comparison as the most widely used methods for the lipidomic analysis together with ultrahigh-performance supercritical fluid chromatography/MS (UHPSFC/MS) method showing promising results in metabolomics analyses. The nontargeted analysis of pooled samples is performed using all tested methods and 610 lipid species within 23 lipid classes are identified. DI method provides the most comprehensive results due to identification of some polar lipid classes, which are not identified by UHPLC and UHPSFC methods. On the other hand, UHPSFC method provides an excellent sensitivity for less polar lipid classes and the highest sample throughput within 10min method time. The sample consumption of DI method is 125 times higher than for other methods, while only 40μL of organic solvent is used for one sample analysis compared to 3.5mL and 4.9mL in case of UHPLC and UHPSFC methods, respectively. Methods are validated for the quantitative lipidomic analysis of plasma samples with one internal standard for each lipid class. Results show applicability of all tested methods for the lipidomic analysis of biological samples depending on the analysis requirements

  5. Investigation of Pinus mugo essential oil oxygenated fraction by combined use of gas chromatography and dry column chromatography.

    Science.gov (United States)

    A, M B; Coran, S A; Giannellini, V; Vincieri, F F; Moneti, G

    1981-09-01

    The oxygenated compounds of Pinus mugo Turra essential oil were investigated by a combination of GC and dry column chromatography (DCC) coordinated by GC data processing. The collected data resulted in a bar graph ("normalized" gas chromatogram) giving the RRT's and relative amounts of 68 components; 38 of them were identified by MS and IR. The described procedure may be used for essential oil analysis in general.

  6. Separation of Aeruginosin-865 from Cultivated Soil Cyanobacterium (Nostoc sp.) by Centrifugal Partition Chromatography combined with Gel Permeation Chromatography

    Czech Academy of Sciences Publication Activity Database

    Cheel, José; Minceva, M.; Urajová, Petra; Aslam, R.; Hrouzek, Pavel; Kopecký, Jiří

    2015-01-01

    Roč. 10, č. 10 (2015), s. 1719-1722 ISSN 1934-578X R&D Projects: GA MŠk EE2.3.30.0059; GA MŠk ED2.1.00/03.0110; GA MŠk LO1416 Institutional support: RVO:61388971 Keywords : Cyanobacteria * Aeruginosin-865 * Centrifugal partition chromatography Subject RIV: EE - Microbiology, Virology Impact factor: 0.884, year: 2015

  7. Comparison of different transition metal ions for immobilized metal affinity chromatography of selenoprotein P from human plasma

    DEFF Research Database (Denmark)

    Sidenius, U; Farver, O; Jøns, O

    1999-01-01

    Cu2+, Ni2+, Zn2+, Co2+ and Cd2+ were evaluated in metal ion affinity chromatography for enrichment of selenoprotein P, and immobilized Co2+ affinity chromatography was found to be the most selective chromatographic method. The chromatography was performed by fast protein liquid chromatography...

  8. Separation of Aeruginosin-865 from Cultivated Soil Cyanobacterium (Nostoc sp.) by Centrifugal Partition Chromatography combined with Gel Permeation Chromatography.

    Science.gov (United States)

    Cheel, José; Minceva, Mirjana; Urajová, Petra; Aslam, Rabya; Hrouzek, Pavel; Kopecký, Jiří

    2015-10-01

    Aeruginosin-865 was isolated from cultivated soil cyanobacteria using a combination of centrifugal partition chromatography (CPC) and gel permeation chromatography. The solubility of Aer-865 in different solvents was evaluated using the conductor-like screening model for real solvents (COSMO-RS). The CPC separation was performed in descending mode with a biphasic solvent system composed of water-n-BuOH-acetic acid (5:4:1, v/v/v). The upper phase was used as a stationary phase, whereas the lower phase was employed as a mobile phase at a flow rate of 10 mL/min. The revolution speed and temperature of the separation column were 1700 rpm and 25 degrees C, respectively. Preparative CPC separation followed by gel permeation chromatography was performed on 50 mg of crude extract yielding Aer-865 (3.5 mg), with a purity over 95% as determined by HPLC. The chemical identity of the isolated compound was confirmed by comparing its spectroscopic data (UV, HRESI-MS, HRESI-MS/MS) with those of an authentic standard and data available in the literature.

  9. Comparison of gas chromatography/isotope ratio mass spectrometry and liquid chromatography/isotope ratio mass spectrometry for carbon stable-isotope analysis of carbohydrates

    NARCIS (Netherlands)

    Moerdijk-Poortvliet, Tanja C. W.; Schierbeek, Henk; Houtekamer, Marco; van Engeland, Tom; Derrien, Delphine; Stal, Lucas J.; Boschker, Henricus T. S.

    2015-01-01

    We compared gas chromatography/isotope ratio mass spectrometry (GC/IRMS) and liquid chromatography/isotope ratio mass spectrometry (LC/IRMS) for the measurement of δ(13)C values in carbohydrates. Contrary to GC/IRMS, no derivatisation is needed for LC/IRMS analysis of carbohydrates. Hence, although

  10. Comparison of gas chromatography/isotope ratio mass spectrometry and liquid chromatography/isotope ratio mass spectrometry for carbon stable-isotope analysis of carbohydrates

    NARCIS (Netherlands)

    Moerdijk-Poortvliet, T.C.W.; Schierbeek, H.; Houtekamer, M.; van Engeland, T.; Derrien, D.; Stal, L.J.; Boschker, H.T.S.

    2015-01-01

    We compared gas chromatography/isotope ratio mass spectrometry (GC/IRMS) and liquid chromatography/isotope ratio mass spectrometry (LC/IRMS) for the measurement of d13C values in carbohydrates. Contrary to GC/IRMS, no derivatisation is needed for LC/IRMS analysis of carbohydrates. Hence, although

  11. Comparison of gas chromatography/isotope ratio mass spectrometry and liquid chromatography/isotope ratio mass spectrometry for carbon stable-isotope analysis of carbohydrates

    NARCIS (Netherlands)

    Moerdijk-Poortvliet, T.C.W.; Schierbeek, H.; Houtekamer, M.; van Engeland, T.; Derrien, D.; Stal, L.J.; Boschker, H.T.S.

    2015-01-01

    Rationale: We compared gas chromatography/isotope ratio mass spectrometry (GC/IRMS) and liquid chromatography/isotope ratio mass spectrometry (LC/IRMS) for the measurement of δ13C values in carbohydrates. Contrary to GC/IRMS, no derivatisation is needed for LC/IRMS analysis of carbohydrates. Hence,

  12. Isolation and purification of six iridoid glycosides from gardenia jasminoides fruit by medium-pressure liquid chromatography combined with macroporous resin chromatography.

    Science.gov (United States)

    Wang, Yun; Liu, Hui; Shen, Lifeng; Yao, Lan; Ma, Yinlian; Yu, Dingrong; Chen, Jianhong; Li, Puling; Chen, Ying; Zhang, Cun

    2015-12-01

    Gardeniae fructus is one of the most frequently used herbs in traditional Chinese medicine. In the present study, a process for the enrichment of six iridoid glycosides from Gardeniae fructus was developed using medium-pressure liquid chromatography combined with macroporous resin and reversed-phase chromatography. The purities of different fractions from Gardeniae fructus were assessed using quantitative high-performance liquid chromatography. After fractionation using HPD-100 column chromatography, a 30% ethanol fraction was selected based on high-performance liquid chromatography and liquid chromatography with mass spectrometry qualitative analysis to separate and purify. Based on the orientation analysis results, six compounds-deacetyl asperulosidic acid methyl ester, gardenoside, ixoroside, scandoside methyl ester, genipin-1-O-β-d-gentiobioside, and geniposide-were successfully isolated and purified in three to four combined steps from Gardeniae fructus. The purities of these compounds were found by high-performance liquid chromatography analysis to be 97.9, 98.1, 95.5, 96.3, 97.1, and 98.7%, respectively. Moreover, their structures were elucidated by NMR spectroscopy and liquid chromatography with tandem mass spectrometry. The separation process was highly efficient, rapid, and accurate, making it a potential approach for the large-scale production of iridoids in the laboratory and providing several marker compounds for quality control. This procedure may be meaningful for the purification of other natural products used in traditional Chinese medicine. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Use and practice of achiral and chiral supercritical fluid chromatography in pharmaceutical analysis and purification.

    Science.gov (United States)

    Lemasson, Elise; Bertin, Sophie; West, Caroline

    2016-01-01

    The interest of pharmaceutical companies for complementary high-performance chromatographic tools to assess a product's purity or enhance this purity is on the rise. The high-throughput capability and economic benefits of supercritical fluid chromatography, but also the "green" aspect of CO2 as the principal solvent, render supercritical fluid chromatography very attractive for a wide range of pharmaceutical applications. The recent reintroduction of new robust instruments dedicated to supercritical fluid chromatography and the progress in stationary phase technology have also greatly benefited supercritical fluid chromatography. Additionally, it was shown several times that supercritical fluid chromatography could be orthogonal to reversed-phase high-performance liquid chromatography and could efficiently compete with it. Supercritical fluid chromatography is an adequate tool for small molecules of pharmaceutical interest: synthetic intermediates, active pharmaceutical ingredients, impurities, or degradation products. In this review, we first discuss about general chromatographic conditions for supercritical fluid chromatography analysis to better suit compounds of pharmaceutical interest. We also discuss about the use of achiral and chiral supercritical fluid chromatography for analytical purposes and the recent applications in these areas. The use of preparative supercritical fluid chromatography by pharmaceutical companies is also covered. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Enabling Microliquid Chromatography by Microbead Packing of Microchannels

    Science.gov (United States)

    Balvin, Manuel; Zheng, Yun

    2014-01-01

    The microbead packing is the critical element required in the success of on-chip microfabrication of critical microfluidic components for in-situ analysis and detection of chiral amino acids. In order for microliquid chromatography to occur, there must be a stationary phase medium within the microchannel that interacts with the analytes present within flowing fluid. The stationary phase media are the microbeads packed by the process discussed in this work. The purpose of the microliquid chromatography is to provide a lightweight, low-volume, and low-power element to separate amino acids and their chiral partners efficiently to understand better the origin of life. In order to densely pack microbeads into the microchannels, a liquid slurry of microbeads was created. Microbeads were extracted from a commercially available high-performance liquid chromatography column. The silica beads extracted were 5 microns in diameter, and had surface coating of phenyl-hexyl. These microbeads were mixed with a 200- proof ethanol solution to create a microbead slurry with the right viscosity for packing. A microfilter is placed at the outlet via of the microchannel and the slurry is injected, then withdrawn across a filter using modified syringes. After each injection, the channel is flushed with ethanol to enhance packing. This cycle is repeated numerous times to allow for a tightly packed channel of microbeads. Typical microbead packing occurs in the macroscale into tubes or channels by using highly pressurized systems. Moreover, these channels are typically long and straight without any turns or curves. On the other hand, this method of microbead packing is completed within a microchannel 75 micrometers in diameter. Moreover, the microbead packing is completed into a serpentine type microchannel, such that it maximizes microchannel length within a microchip. Doing so enhances the interactions of the analytes with the microbeads to separate efficiently amino acids and amino acid

  15. Modelling of Zirconium and Hafnium separation using continuous annular chromatography

    International Nuclear Information System (INIS)

    Moch-Setyadji; Endang Susiantini

    2014-01-01

    Nuclear degrees of zirconium in the form of a metal alloy is the main material for fuel cladding of NPP. Zirconium is also used as sheathing UO 2 kernel in the form of ZrC as a substitute of SiC in the fuel elements of High Temperature Reactor (HTR). Difficulty separating hafnium from zirconium because it has a lot of similarities in the chemical properties of Zr and Hf. Annular chromatography is a device that can be used for separating of zirconium and hafnium to obtain zirconium nuclear grade. Therefore, it is necessary to construct the mathematical modelling that can describe the separation of zirconium and hafnium in the annular chromatography containing anion resin dowex-1X8. The aim of research is to perform separation simulation by using the equilibrium model and mass transfer coefficient resulted from research. Zr and Hf feed used in this research were 26 and 1 g/l, respectively. Height of resin (L), angular velocity (ω) and the superficial flow rate (uz) was varied to determine the effect of each parameter on the separation of Zr and Hf. By using Kd and Dv values resulted previous research. Simulation results showed that zirconium and hafnium can be separated using a continuous annular chromatography with high resin (long bed) 50 cm, superficial flow rate of 0.001 cm/s, the rotation speed of 0.006 rad/min and 20 cm diameter annular. In these conditions the results obtained zirconium concentration of 10,303.226 g/m 3 and hafnium concentration of 12.324 g/m 3 (ppm). (author)

  16. Mixed-mode chromatography/isotope ratio mass spectrometry.

    Science.gov (United States)

    McCullagh, James S O

    2010-03-15

    Liquid chromatography coupled to molecular mass spectrometry (LC/MS) has been a standard technique since the early 1970s but liquid chromatography coupled to high-precision isotope ratio mass spectrometry (LC/IRMS) has only been available commercially since 2004. This development has, for the first time, enabled natural abundance and low enrichment delta(13)C measurements to be applied to individual analytes in aqueous mixtures creating new opportunities for IRMS applications, particularly for the isotopic study of biological molecules. A growing number of applications have been published in a range of areas including amino acid metabolism, carbohydrates studies, quantification of cellular and plasma metabolites, dietary tracer and nucleic acid studies. There is strong potential to extend these to new compounds and complex matrices but several challenges face the development of LC/IRMS methods. To achieve accurate isotopic measurements, HPLC separations must provide baseline-resolution between analyte peaks; however, the design of current liquid interfaces places severe restrictions on compatible flow rates and in particular mobile phase compositions. These create a significant challenge on which reports associated with LC/IRMS have not previously focused. Accordingly, this paper will address aspects of chromatography in the context of LC/IRMS, in particular focusing on mixed-mode separations and their benefits in light of these restrictions. It aims to provide an overview of mixed-mode stationary phases and of ways to improve high aqueous separations through manipulation of parameters such as column length, temperature and mobile phase pH. The results of several practical experiments are given using proteogenic amino acids and nucleosides both of which are of noted importance in the LC/IRMS literature. This communication aims to demonstrate that mixed-mode stationary phases provide a flexible approach given the constraints of LC/IRMS interface design and acts as a

  17. The high pressure electronic control system in liquid chromatography

    International Nuclear Information System (INIS)

    Popescu, Stefan; Popeneciu, Gabriel; Toadere, Florin

    2002-01-01

    The Liquid Chromatography system can perform a wide variety of measurements and separations especially for the organic liquids, with maximum applications flexibility for less than half price of Gas Chromatography. The repeatability and accuracy of results in quantitative high pressure liquid chromatography are highly dependent on the reproducibility and accuracy of both integrated flow rate and mobile phase composition. Flow rate fluctuation leads to poor reproducibility in both integrated peak areas and retention times. Similarly, poor control of mobile phase composition will cause poor repeatability of retention time and peak heights. The our Solvent Delivery System SDS 200 is a single pump system which provides precise compensated flow rates from 0.01 to 10 mL/min, selectable upper pressure limits of 0 to 100 bar or 10 to 450 bar, and solvent compressibility correction. Ternary solvent system on-line mixing capability saves time, reduces solvent waste and provides more flexibility for difficult separations. The pump itself has two different displacement pistons which are used alternately on both suction and discharge, so that intake of the solvent are synchronous. The evacuated solvent from the two pump's chambers is mixed in the reference unit and then is supplied to the damping unit for flow ripple reduction. The SDS Electronic Module ensures the functions: controls the programmed flow rate, detects and shows the solvent pressure in solvent, supplies the step motor, measures and limits the solvent pressure. The control panel of SDS 200, contains a two-stages flow decimal programmer, a eight-positions knob for upper pressure limits, an alarm LED and a parallel port for connection to a PC system. (authors)

  18. Simultaneous determination of inorganic and organic anions by ion chromatography

    International Nuclear Information System (INIS)

    Park, Yang Soon; Joe, Ki Soo; Han, Sun Ho; Park, Soon Dal; Choi, Kwang Soon

    1999-06-01

    Four methods were investigated for the simultaneous determination of several inorganic and organic anions in aqueous solution by ion chromatography. The first is two columns coupled system. The second is the gradient elution system with an anion exchange column. The third is the system with a mixed-mode stationary phase. The fourth is the system with an anion exchange column and the eluant of low conductivity without ion suppressor. The advantages and disadvantages of individual systems were discussed. The suitable methods were proposed for the application to the samples of the nuclear power industry and the environment. (author)

  19. Characterization of some Jordanian oil shales by pyrolysis gas chromatography

    International Nuclear Information System (INIS)

    Jaradat, Q. M.

    1995-01-01

    Gas chromatography with flame ionization detector (GC-FID) was used to study pyrolysis of some Jordanian oil shale samples. Three sampls of different altitudes from El-Lajjun were studied. Pyrograms of solid sampls were studied at different temperature profiles. Solid-liquid extraction with water, methanol, or hexane allowed extraction of organics of different polarity. Hexane showed the highest extraction efficiency. Reproducibility of the pyrograms of the solid sample was evalualted. Relative standard deviation was 7.56%. (author). 7 refs., 8 figs

  20. Determination of inorganic ions in natural water by ion chromatography

    International Nuclear Information System (INIS)

    Nazaratul Ashifa Abdullah Salim; Mohd Suhaimi Hamzah; Eewiat Edin Put; Abdul Khalik Wood; Shamsiah Abdul Rahman; Md Suhaimi Elia

    2010-01-01

    Ion chromatography (IC) is a well established methodology for analysis of ionic species. The concentration of ionic species was determined using suppressed IC with conductivity detection. Anion species were determined in a single 15-min run with Na 2 CO 3 and NaHCO 3 eluent. Cation species were analysed by direct injection of 1 ml and isocratic elution with a methanesulfonic acid (MSA) eluent. Natural water were collected from various sources such as rainwater, lake, river and groundwater. Analysis performance of IC system was validated by evaluating the linear regression of calibration curve, recovery of spike sample and quality control sample. (author)

  1. Isotopic enrichment of 15N by ionic exchange chromatography

    International Nuclear Information System (INIS)

    Trivelin, P.C.O.

    1979-01-01

    The present paper presents some studies on production of 15 N-enriched ammonium sulphate with 5% atoms by ionic exchange chromatography method. Two systems are described of columns of resin, where experiments were conducted by eluition of NH 4 + bands with sodium hydroxide solution. Analyses were made of the cost of production of 15 N-enriched ammonium sulphate 5% atoms and, based on the experiments developed, a cost was obtained which was compatible with the international price of the product. The isotopic analyses of nitrogen were made by mass spectrometry. (Author) [pt

  2. High-performance liquid chromatography of human glycoprotein hormones.

    Science.gov (United States)

    Chlenov, M A; Kandyba, E I; Nagornaya, L V; Orlova, I L; Volgin, Y V

    1993-02-12

    The chromatographic behavior of the glycoprotein hormones from human pituitary glands and of placental origin [thyroid-stimulating hormone, luteinizing hormone and chorionic gonadotropin (CG)] was studied. It was shown that hydrophobic interaction chromatography on a microparticulate packing and anion-exchange HPLC can be applied for the purification of these hormones. Reversed-phase HPLC on wide-pore C4-bonded silica at neutral pH can be applied for the determination of the above hormones and for the isolation of pure CG and its subunits.

  3. Gas chromatography of alkylphosphonic and dialkyl phosphinic acids

    International Nuclear Information System (INIS)

    Gasco Sanchez, L.; Barrera Pinero, R.; Ramirez Caceres, A.; Martin Munoz, M.

    1978-01-01

    After carrying out an optimization study on the separation conditions for the TMSr- derivatives, of the hexyl-, cyclohexyl-, heptyl-, and octyl-phosphonic acids; dihexyl dicyclohexyl-, heptyl-, and octyl-phosphinic acids, and dioctyl phosphine oxide, their retention indices (I) at two temperatures and on the OV-1 and OV-17 stationary phase were determined. Correlations between I and molecular structure were established. Calibration factors of these compounds in the flame ionization detector were studied, and the results analyzed taking into account the variables affecting the quantitative results, These results were unbiased but they had a lower precision than that usually achievable in gas chromatography. (Author) 24 refs

  4. Diesel characterization by high-resolution mass spectrometry - gas chromatography

    International Nuclear Information System (INIS)

    Baldrich, C.A

    1998-01-01

    High-resolution mass spectrometry-gas chromatography is combined with the HC22 method in order to obtain detailed information about the chemical composition of diesel and the distribution of different compound types in terms of its final boiling temperature from a single analysis. The total time elapsed from sample injection and signal processing to obtain final results is 90 minutes. This fact makes this methodology a new and very important tool for the decision making process concerning the most suitable final boiling temperature and the type of treatment of the product in order to obtain diesel that fulfills the international standards. The consistency and repeatability of the experimental results are demonstrated

  5. Size-exclusion chromatography and its optimization for material science

    Czech Academy of Sciences Publication Activity Database

    Netopilík, Miloš; Trhlíková, Olga

    2017-01-01

    Roč. 6, č. 4 (2017), s. 1-5, č. článku 358. ISSN 2169-0022 R&D Projects: GA ČR(CZ) GC17-04258J Grant - others:OPPK(XE) CZ.2.16/3.1.00/24504; AV ČR(CZ) ASRT-16-02 Program:Bilaterální spolupráce Institutional support: RVO:61389013 Keywords : separation mechanism * size-exclusion chromatography * band broadening function Subject RIV: CD - Macromolecular Chemistry OBOR OECD: Polymer science

  6. Magnesium isotope fractionation in cation-exchange chromatography

    International Nuclear Information System (INIS)

    Oi, T.; Yanase, S.; Kakihana, H.

    1987-01-01

    Band displacement chromatography of magnesium has been carried out successfully for the purpose of magnesium isotope separation by using a strongly acidic cation-exchange resin and the strontium ion as the replacement ion. A small but definite accumulation of the heavier isotopes ( 25 Mg, 26 Mg) has been observed at the front parts of the magnesium chromatograms. The heavier isotopes have been fractionated preferentially into the solution phase. The single-stage separation factors have been calculated for the 25 Mg/ 24 Mg and 26 Mg/ 24 isotopic pairs at 25 0 C. The reduced partition function ratios of magnesium species involved in the present study have been estimated

  7. Physicochemical characterization of some solid materials by inverse gas chromatography

    International Nuclear Information System (INIS)

    Hamieh, T.; Abdessater, S.

    2004-01-01

    Full text.New equations and models on two-dimensional state of solid surfaces were previously elaborated in many other studies. results obtained were used in this paper to the determination and the quantification of some physicochemical properties of some solid surfaces, and especially, to study the acid-base superficial characteristics of some solid substrates like oxides and/or polymer adsorbed on oxides, carbon fibers, cements, etc. The technique used was the inverse gas chromatography (CGI) at infinite dilution. The acid-base constants were calculated for many solid surfaces: Al 2 O 3 , SiO 2 , MgO, ZnO, some cements, textiles and carbon fibers

  8. Separation of alkali metals by extraction chromatography using polyethers

    International Nuclear Information System (INIS)

    Smulek, W.; Lada, W.

    1977-01-01

    Separation of alkali metals by means of acyclic polyether 1,13 bis(8-chinolinyl)1, 4, 7, 10, 13 pentaoxatridecane (CPOD) and cyclic polyether dibenzo-18-crown-6 (DBC) using extraction chromatography is described. Solutions of NaSCN were used as eluting agents. The separation ability of the polyether columns strongly depends on pH and temperature. The following radioisotopes were used in the experiments: Na-22, K-42, Rb-86 and Cs-137. The radionuclide purity of the tracers was examined by γ-ray spectrometry using a Ge(Li) detector and a multichannel γ-ray spectrometer. (T.I.)

  9. Establishment of analysis method for methane detection by gas chromatography

    Science.gov (United States)

    Liu, Xinyuan; Yang, Jie; Ye, Tianyi; Han, Zeyu

    2018-02-01

    The study focused on the establishment of analysis method for methane determination by gas chromatography. Methane was detected by hydrogen flame ionization detector, and the quantitative relationship was determined by working curve of y=2041.2x+2187 with correlation coefficient of 0.9979. The relative standard deviation of 2.60-6.33% and the recovery rate of 96.36%∼105.89% were obtained during the parallel determination of standard gas. This method was not quite suitable for biogas content analysis because methane content in biogas would be over the measurement range in this method.

  10. Application of ion chromatography in the analysis of metals

    International Nuclear Information System (INIS)

    Doepke, T.; Braun, N.; Wuensch, G.

    1992-01-01

    Methods for the determination of chloride in molybdenum, tungsten, niobium and tantalum and of phosphorus in molybdenum and tungsten are presented. After oxidative digestion the analytes are separated from the matrix and accumulated in a small volume of liquid. Unsuppressed ion chromatography serves as the final determination method. The trace-matrix-separation and enrichment of chloride is largely independent of the kind of matrix. The procedure is therefore also applicable to concentrated solutions of various salts. A closed system ensures chloride blanks around 0.2 ppm and detection limits in the higher ppb range. A modification allows an enrichment of bromide and the simultaneous determination of chloride and bromide. (orig.) [de

  11. Applications of pressurized cation exchange chromatography for fission yield determination

    International Nuclear Information System (INIS)

    Yan Shuheng; Lin Fa; Zhang Hongdi; Li Xueliang; Zhang Shulan

    1988-01-01

    In order to determine the fission yields of lanthanides precisely, lanthanides with carriers of 1-2 mg per element are separated from each other by means of pressurized cation exchange chromatography - αHIBA concentration gradient elution. The effect of initial loading technique, concentration gradient, flow rate, and temperature on separation were investigated in detail. Under the optimum conditions adapted according to the results given in this work, all the lanthanides can be completely separated within about 90 minutes with a recovery of more than 95% and purity higher than 99%. (author) 3 refs.; 6 figs

  12. Analysis of artificial fireplace logs by high temperature gas chromatography.

    Science.gov (United States)

    Kuk, Raymond J

    2002-11-01

    High temperature gas chromatography is used to analyze the wax of artificial fireplace logs (firelogs). Firelogs from several different manufacturers are studied and compared. This study shows that the wax within a single firelog is homogeneous and that the wax is also uniform throughout a multi-firelog package. Different brands are shown to have different wax compositions. Firelogs of the same brand, but purchased in different locations, also have different wax compositions. With this information it may be possible to associate an unknown firelog sample to a known sample, but a definitive statement of the origin cannot be made.

  13. [Determination of acetochlor and oxyfluorfen by capillary gas chromatography].

    Science.gov (United States)

    Xiang, Wen-Sheng; Wang, Xiang-Jing; Wang, Jing; Wang, Qing

    2002-09-01

    A method is described for the determination of acetochlor and oxyfluorfen by capillary gas chromatography with FID and an SE-30 capillary column (60 m x 0.53 mm i. d., 1.5 microm), using dibutyl phthalate as the internal standard. The standard deviations for acetochlor and oxyfluorfen concentration(mass fraction) were 0.44% and 0.47% respectively. The relative standard deviations for acetochlor and oxyfluorfen were 0.79% and 0.88% and the average recoveries for acetochlor and oxyfluorfen were 99.3% and 101.1% respectively. The method is simple, rapid and accurate.

  14. Capillary supercritical fluid chromatography - Fourier transform infrared spectrometry

    International Nuclear Information System (INIS)

    Olesik, S.V.; French, S.B.; Movotny, M.

    1984-01-01

    One of the most demanding tasks asked of an analytical chemist today is to separate and identify the components of a nonvolatile complex mixture. An efficient separation technique combined with a universal detector that provides structural information, therefore, would be a great asset to analytical chemists. Capillary supercritical fluid chromatography (SFC) - Fourier transform infrared spectrometry (FTIR) shows great potential for being such a technique. SFC-FTIR shows great potential as a very powerful technique for separation and identification of thermally labile and nonvolatile compounds. Research is continuing in these labs to further optimize the technique. 2 refs

  15. Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography.

    OpenAIRE

    Guerrant, G O; Lambert, M A; Moss, C W

    1982-01-01

    A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent ...

  16. Hyphenated techniques in multidimensional gas chromatography for analysis of wine aroma and related odorants

    OpenAIRE

    Chin, Sung Tong

    2017-01-01

    The advent of analytical multidimensional gas chromatography (MDGC) techniques, with recent development in comprehensive two-dimensional gas chromatography (GC×GC) as well as microfluidic technology in conventional heart-cut MDGC, has achieved excellent separation efficiency for advanced characterization of complex volatile and semi-volatile samples, which is unlikely to be accomplished by single dimensional chromatography. This dissertation work has highlighted the potential opportunities fo...

  17. Overview of online two-dimensional liquid chromatography based on cell membrane chromatography for screening target components from traditional Chinese medicines.

    Science.gov (United States)

    Muhammad, Saqib; Han, Shengli; Xie, Xiaoyu; Wang, Sicen; Aziz, Muhammad Majid

    2017-01-01

    Cell membrane chromatography is a simple, specific, and time-saving technique for studying drug-receptor interactions, screening of active components from complex mixtures, and quality control of traditional Chinese medicines. However, the short column life, low sensitivity, low column efficiency (so cannot resolve satisfactorily mixture of compounds), low peak capacity, and inefficient in structure identification were bottleneck in its application. Combinations of cell membrane chromatography with multidimensional chromatography such as two-dimensional liquid chromatography and high sensitivity detectors like mass have significantly reduced many of the above-mentioned shortcomings. This paper provides an overview of the current advances in online two-dimensional-based cell membrane chromatography for screening target components from traditional Chinese medicines with particular emphasis on the instrumentation, preparation of cell membrane stationary phase, advantages, and disadvantages compared to alternative approaches. The last section of the review summarizes the applications of the online two-dimensional high-performance liquid chromatography based cell membrane chromatography reported since its emergence to date (2010-June 2016). © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Purification of flavonoids from licorice using an off-line preparative two-dimensional normal-phase liquid chromatography/reversed-phase liquid chromatography method.

    Science.gov (United States)

    Fan, Yunpeng; Fu, Yanhui; Fu, Qing; Cai, Jianfeng; Xin, Huaxia; Dai, Mei; Jin, Yu

    2016-07-01

    An orthogonal (71.9%) off-line preparative two-dimensional normal-phase liquid chromatography/reversed-phase liquid chromatography method coupled with effective sample pretreatment was developed for separation and purification of flavonoids from licorice. Most of the nonflavonoids were firstly removed using a self-made Click TE-Cys (60 μm) solid-phase extraction. In the first dimension, an industrial grade preparative chromatography was employed to purify the crude flavonoids. Click TE-Cys (10 μm) was selected as the stationary phase that provided an excellent separation with high reproducibility. Ethyl acetate/ethanol was selected as the mobile phase owing to their excellent solubility for flavonoids. Flavonoids co-eluted in the first dimension were selected for further purification using reversed-phase liquid chromatography. Multiple compounds could be isolated from one normal-phase fraction and some compounds with bad resolution in one-dimensional liquid chromatography could be prepared in this two-dimensional system owing to the orthogonal separation. Moreover, this two-dimensional liquid chromatography method was beneficial for the preparation of relatively trace flavonoid compounds, which were enriched in the first dimension and further purified in the second dimension. Totally, 24 flavonoid compounds with high purity were obtained. The results demonstrated that the off-line two-dimensional liquid chromatography method was effective for the preparative separation and purification of flavonoids from licorice. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. Use of micro gas chromatography in the fuel cycle of fusion reactors

    International Nuclear Information System (INIS)

    Laesser, R.; Gruenhagen, S.; Kawamura, Y.

    2003-01-01

    Various analytical techniques exist to determine the compositions of gases handled in the fuel cycle of future fusion machines. Gas chromatography was found to be the most appropriate method. The main disadvantages of conventional gas chromatography were the long retention times for the heavy hydrogen species of >30 min. Recent progress in the development of micro-gas chromatography has reduced these retention times to ∼3 min. The usefulness of micro-gas chromatography for the analysis of hydrogen and impurity gas mixtures in the fuel cycle of future fusion machines is presented and the advantages and drawbacks are discussed

  20. PHARMACEUTICAL AND BIOMEDICAL APPLICATIONS OF AFFINITY CHROMATOGRAPHY: RECENT TRENDS AND DEVELOPMENTS

    Science.gov (United States)

    Hage, David S.; Anguizola, Jeanethe A.; Bi, Cong; Li, Rong; Matsuda, Ryan; Papastavros, Efthimia; Pfaunmiller, Erika; Vargas, John; Zheng, Xiwei

    2012-01-01

    Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents. This method is based on the use of a biologically-related agent as a stationary phase to selectively retain analytes or to study biological interactions. This review discusses the basic principles behind affinity chromatography and examines recent developments that have occurred in the use of this method for biomedical and pharmaceutical analysis. Techniques based on traditional affinity supports are discussed, but an emphasis is placed on methods in which affinity columns are used as part of HPLC systems or in combination with other analytical methods. General formats for affinity chromatography that are considered include step elution schemes, weak affinity chromatography, affinity extraction and affinity depletion. Specific separation techniques that are examined include lectin affinity chromatography, boronate affinity chromatography, immunoaffinity chromatography, and immobilized metal ion affinity chromatography. Approaches for the study of biological interactions by affinity chromatography are also presented, such as the measurement of equilibrium constants, rate constants, or competition and displacement effects. In addition, related developments in the use of immobilized enzyme reactors, molecularly imprinted polymers, dye ligands and aptamers are briefly considered. PMID:22305083

  1. Hyphenated and comprehensive liquid chromatography × gas chromatography-mass spectrometry for the identification of Mycobacterium tuberculosis.

    Science.gov (United States)

    Mourão, Marta P B; Denekamp, Ilse; Kuijper, Sjoukje; Kolk, Arend H J; Janssen, Hans-Gerd

    2016-03-25

    Tuberculosis is one of the world's most emerging public health problems, particularly in developing countries. Chromatography based methods have been used to tackle this epidemic by focusing on biomarker detection. Unfortunately, interferences from lipids in the sputum matrix, particularly cholesterol, adversely affect the identification and detection of the marker compounds. The present contribution describes the serial combination of normal phase liquid chromatography (NPLC) with thermally assisted hydrolysis and methylation followed by gas chromatography-mass spectrometry (THM-GC-MS) to overcome the difficulties of biomarker evaluation. The in-series combination consists of an LC analysis where fractions are collected and then transferred to the THM-GC-MS system. This was either done with comprehensive coupling, transferring all the fractions, or with hyphenated interfacing, i.e. off-line multi heart-cutting, transferring only selected fractions. Owing to the high sensitivity and selectivity of LC as a sample pre-treatment method, and to the high specificity of the MS as a detector, this analytical approach, NPLC × THM-GC-MS, is extremely sensitive. The results obtained indicate that this analytical set-up is able to detect down to 1 × 10(3) mycobacteria/mL of Mycobacterium tuberculosis strain 124, spiked in blank sputum samples. It is a powerful analytical tool and also has great potential for full automation. If further studies demonstrate its usefulness when applied blind in real sputum specimens, this technique could compete with the current smear microscopy in the early diagnosis of tuberculosis. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Method transfer from high-pressure liquid chromatography to ultra-high-pressure liquid chromatography. II. Temperature and pressure effects.

    Science.gov (United States)

    Åsberg, Dennis; Samuelsson, Jörgen; Leśko, Marek; Cavazzini, Alberto; Kaczmarski, Krzysztof; Fornstedt, Torgny

    2015-07-03

    The importance of the generated temperature and pressure gradients in ultra-high-pressure liquid chromatography (UHPLC) are investigated and compared to high-pressure liquid chromatography (HPLC). The drug Omeprazole, together with three other model compounds (with different chemical characteristics, namely uncharged, positively and negatively charged) were used. Calculations of the complete temperature profile in the column at UHPLC conditions showed, in our experiments, a temperature difference between the inlet and outlet of 16 °C and a difference of 2 °C between the column center and the wall. Through van't Hoff plots, this information was used to single out the decrease in retention factor (k) solely due to the temperature gradient. The uncharged solute was least affected by temperature with a decrease in k of about 5% while for charged solutes the effect was more pronounced, with k decreases up to 14%. A pressure increase of 500 bar gave roughly 5% increase in k for the uncharged solute, while omeprazole and the other two charged solutes gave about 25, 20 and 15% increases in k, respectively. The stochastic model of chromatography was applied to estimate the dependence of the average number of adsorption/desorption events (n) and the average time spent by a molecule in the stationary phase (τs) on temperature and pressure on peak shape for the tailing, basic solute. Increasing the temperature yielded an increase in n and decrease in τs which resulted in less skew at high temperatures. With increasing pressure, the stochastic modeling gave interesting results for the basic solute showing that the skew of the peak increased with pressure. The conclusion is that pressure effects are more pronounced for both retention and peak shape than the temperature effects for the polar or charged compounds in our study. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. [Compound preservative and cyclamat determinated by gas chromatography].

    Science.gov (United States)

    Xin, Ruozhu; Ding, Mei; Zheng, Xianguang; Li, Yajuan

    2008-07-01

    To establish a gas chromatography method for synchronous determination of six preservatives and cyclamat in cake and pastry. The pre-treatment of complicated compound was finished by dialysis cleaning technology. The interference of the complicated foundation body in the cake and pastry was removed by the best dialysis condition: 2 g/L NaOH of dialysis fluid was dialyzed at the temperature of 25 degrees C for 24h, and the six preservatives and cyclamat (sorbic acid, benzoic acid, dehydroacetic acid, ethylparabenum, propylparabenum, butylparabenun and sodium cyclamate) were synchronously seperated by 60-80 mesh Chromosorb WAW DMCS glass packing column of intercoated 5% DEGS + 1% H3 PO4. The seven components in the sample had a good lining relation at the concentration 100 -5000 mg/L when the external standard method was used, and their correlation coefficients were 0.9993-0.9998, and their average recovery rates were 87.5%-101.9%, and their relative standard deviations were 0.64%-3.3%, and detection limits were 1.1-8.8 mg/L. This method could be used to quickly analyse the compound preservatives of the large batch cake and pastry through the common gas chromatography and packed column and this method was simple, accurate and quick.

  4. Boron isotope fractionation in column chromatography with glucamine type fibers

    International Nuclear Information System (INIS)

    Sonoda, Akinari; Makita, Yoji; Hirotsu, Takahiro

    2008-01-01

    Glucamine type polymers have specific affinity toward boric acid and borate ion. Among them, Chelest Fiber GRY-L showed larger fractionation for boron isotopes than other polymers in our previous study. For this study, we used Chelest Fibers with different fiber lengths (1.0 mm, 0.5 mm, and 0.3 mm) as column packing materials to perform chromatographic separation of boron isotopes. The shorter fiber has larger packing density when packed into the column using a dry method. The 0.3-mm-long fiber has a larger backpressure than fibers of other lengths. Boron adsorption capacities were measured using the breakthrough operation. At this time, the 0.5-mm-long fiber showed the highest capacity. When we measured the isotope ratio profile for fibers of different length using column chromatography, 0.5-mm-long fibers displayed the highest boron isotope fractionation. The 0.5-mm-long fiber is promising as a packing material of column chromatography for boron isotope separation. We also changed operation methods. The lower eluent concentration and the slower flow rate are suitable for boron isotope separation. (author)

  5. Determination of pertechnetate by liquid chromatography with reductive electrochemical detection

    International Nuclear Information System (INIS)

    Lewis, J.Y.; Zodda, J.P.; Deutsch, E.; Heineman, W.R.

    1983-01-01

    A method utilizing liquid chromatography with electrochemical detection has been developed for the determination of total TcO 4 - (/sup 99m/TcO 4 - and /sup 99m/TcO 4 - ) in 99 Mo//sup 99m/Tc generator eluents. Pertechnetate, which is the starting material for the preparation of many diagnostic radiopharmaceuticals, is generally present in these eluents in the concentration range of 5 X 10 -8 M to 5 X 10 -6 M. No sample pretreatment is necessary since impurities and other components are separated by the high-pressure liquid chromatography (HPLC) NH 2 -bonded column. By use of both static mercury drop (SMDE) and solid electrode detectors, in conjuction with rigorous deoxygenation procedures, total TcO 4 - in generator eluents is readily determined. A severe electrode fouling phenomenon limits the use of solid electrode detectors to TcO 4 - concentrations less than 10 -7 M, the working range for a carbon electrode being 8.5 X 10 -9 to 1.0 X 10 -7 M. The working range for the SMDE is 2.1 X 10 -8 to 1.0 X 10 -4 M TcO 4 -

  6. Chromatography Analysis of Jatropha Curcas L Oil Raw and Refined

    International Nuclear Information System (INIS)

    Chitue de Assuncao Nascimento, Juliano; Lafargue Perez, Francisco; Diaz Velazquez, Manuel

    2013-01-01

    The chromatography of gases is one of the most versatile methods utilized in the chemical laboratory, which permits the determinations of the percentages and types of fatty acids present in vegetable oils. The objective of this work was to carry out a comparative study of the composition of the fatty acids, of the mechanically extracted oil from the seed of the Jatropha curcas L plant in the raw and refined state, using the data obtained by gas chromatography. The fatty acids of the oil were determined as methylic esteres. The analyst was carried out in a gas chromatographer, with a detector of ionization by way of flame (FID), Agilent 7890A (Agilent, USA) coupled to HP Pentium 4 computer, with a data processing program. The temperatures of the injector and of the detector were 250 °C and 260 °C respectively. The data obtained chromatographically of the oil of Jatropha curcas L, raw and refined indicates that the refinery process did not vary the composition of the fatty acid, because there are no significant differences between them. Also, the results obtained by other investigators, showed that the predominant fatty acids obtained from the oil of the Jatropha curcas L were palmitic and stearic in the saturated fatty acids, and oleic and linoleic in the unsaturated fatty acids

  7. Chromatography analysis of Jatropha Curcas L oil raw and refined

    International Nuclear Information System (INIS)

    Chitue de Assuncao Nascimento, Juliano; Lafargue Perez, Francisco; Diaz Velazquez, Manuel

    2013-01-01

    The chromatography of gases is one of the most versatile methods utilized in the chemical laboratory, which permits the determinations of the percentages and types of fatty acids present in vegetable oils. The objective of this work was to carry out a comparative study of the composition of the fatty acids, of the mechanically extracted oil from the seed of the Jatropha curcas L plant in the raw and refined state, using the data obtained by gas chromatography. The fatty acids of the oil were determined as methylic esteres. The analyst was carried out in a gas chromatographer, with a detector of ionization by way of flame (FID), Agilent 7890A (Agilent, USA) coupled to HP Pentium 4 computer, with a data processing program. The temperatures of the injector and of the detector were 250℃ and 260℃ respectively. The data obtained chromatographically of the oil of Jatropha curcas L, raw and refined indicates that the refinery process did not vary the composition of the fatty acid, because there are no significant differences between them. Also, the results obtained by other investigators, showed that the predominant fatty acids obtained from the oil of the Jatropha curcas L were palmitic and stearic in the saturated fatty acids, and oleic and linoleic in the unsaturated fatty acids

  8. Cortisol production rates measured by liquid chromatography/mass spectrometry

    International Nuclear Information System (INIS)

    Esteban, N.V.; Yergey, A.L.

    1990-01-01

    Cortisol production rates (FPRs) in physiologic and pathologic states in humans have been investigated over the past 30 years. However, there has been conflicting evidence concerning the validity of the currently accepted value of FPRs in humans (12 to 15 mg/m2/d) as determined by radiotracer methodology. The present study reviews previous methods proposed for the measurement of FPRs in humans and discusses the applications of the first method for the direct determination of 24-hour plasma FPRs during continuous administration of a stable isotope, using a thermospray high-pressure liquid chromatography-mass spectrometry technique. The technique is fast, sensitive, and, unlike gas chromatography-mass spectrometry methods, does not require derivatization, allowing on-line detection and quantification of plasma cortisol after a simple extraction procedure. The results of determination of plasma FPRs by stable tracer/mass spectrometry are directly in units of mass/time and, unlike radiotracer methods, are independent of any determination of volume of distribution or cortisol concentration. Our methodology offers distinct advantages over radiotracer techniques in simplicity and reliability since only single measurements of isotope ratios are required. The technique was validated in adrenalectomized patients. Circadian variations in daily FRPs were observed in normal volunteers, and, to date, results suggest a lower FRP in normal children and adults than previously believed. 88 references

  9. Porcine Circovirus (PCV) Removal by Q Sepharose Fast Flow Chromatography

    Science.gov (United States)

    Yang, Bin; Wang, Hua; Ho, Cintia; Lester, Philip; Chen, Qi; Neske, Florian; Baylis, Sally A; Blümel, Johannes

    2013-01-01

    The recently discovered contamination of oral rotavirus vaccines led to exposure of millions of infants to porcine circovirus (PCV). PCV was not detected by conventional virus screening tests. Regulatory agencies expect exclusion of adventitious viruses from biological products. Therefore, methods for inactivation/removal of viruses have to be implemented as an additional safety barrier whenever feasible. However, inactivation or removal of PCV is difficult. PCV is highly resistant to widely used physicochemical inactivation procedures. Circoviruses such as PCV are the smallest viruses known and are not expected to be effectively removed by currently-used virus filters due to the small size of the circovirus particles. Anion exchange chromatography such as Q Sepharose® Fast Flow (QSFF) has been shown to effectively remove a range of viruses including parvoviruses. In this study, we investigated PCV1 removal by virus filtration and by QSFF chromatography. As expected, PCV1 could not be effectively removed by virus filtration. However, PCV1 could be effectively removed by QSFF as used during the purification of monoclonal antibodies (mAbs) and a log10 reduction value (LRV) of 4.12 was obtained. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1464–1471, 2013 PMID:24039195

  10. Multimodal charge-induction chromatography for antibody purification.

    Science.gov (United States)

    Tong, Hong-Fei; Lin, Dong-Qiang; Chu, Wen-Ning; Zhang, Qi-Lei; Gao, Dong; Wang, Rong-Zhu; Yao, Shan-Jing

    2016-01-15

    Hydrophobic charge-induction chromatography (HCIC) has advantages of high capacity, salt-tolerance and convenient pH-controlled elution. However, the binding specificity might be improved with multimodal molecular interactions. New ligand W-ABI that combining tryptophan and 5-amino-benzimidazole was designed with the concept of mutimodal charge-induction chromatography (MCIC). The indole and benzimidazole groups of the ligand could provide orientated mutimodal binding to target IgG under neutral pH, while the imidazole groups could induce the electrostatic repulsion forces for efficient elution under acidic pH. W-ABI ligand was coupled successfully onto agarose gel, and IgG adsorption behaviors were investigated. High affinity to IgG was found with the saturated adsorption capacity of 70.4 mg/ml at pH 7, and the flow rate of mobile phase showed little impact on the dynamic binding capacity. In addition, efficient elution could be achieved at mild acidic pH with high recovery. Two separation cases (IgG separation from albumin containing feedstock and monoclonal antibody purification from cell culture supernatant) were verified with high purity and recovery. In general, MCIC with the specially-designed ligand is an expanding of HCIC with improved adsorption selectivity, which would be a potential alternative to Protein A-based capture for the cost-effective purification of antibodies. Copyright © 2015 Elsevier B.V. All rights reserved.

  11. Graphene-coated polymeric anion exchangers for ion chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Kai; Cao, Minyi; Lou, Chaoyan [Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028 (China); Wu, Shuchao, E-mail: wushch2002@163.com [Zhejiang Institute of Geology and Mineral Resources, Hangzhou 310007 (China); Zhang, Peimin [Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028 (China); Zhi, Mingyu [Hangzhou Vocational & Technical College, Hangzhou, 310018 (China); Zhu, Yan, E-mail: zhuyan@zju.edu.cn [Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028 (China)

    2017-06-01

    Carbonaceous stationary phases have gained much attention for their peculiar selectivity and robustness. Herein we report the fabrication and application of a graphene-coated polymeric stationary phase for anion exchange chromatography. The graphene-coated particles were fabricated by a facile evaporation-reduction method. These hydrophilic particles were proven appropriate substrates for grafting of hyperbranched condensation polymers (HBCPs) to make pellicular anion exchangers. The new phase was characterized by zeta potentials, Fourier transform infrared spectroscopy, thermogravimetry and scanning electron microscope. Frontal displacement chromatography showed that the capacities of the anion exchangers were tuned by both graphene amount and HBCPs layer count. The chromatographic performance of graphene-coated anion exchangers was demonstrated with separation of inorganic anions, organic acids, carbohydrates and amino acids. Good reproducibility was obtained by consecutive injections, indicating high chemical stability of the coating. - Highlights: • Graphene-coated polymeric particles were fabricated by a facile method. • Hyperbranched condensation polymers (HBCPs) were grafted from graphene-coated particles to make anion exchangers. • Graphene amount and HBCPs layer count had significant effects on the anion exchange capacities. • Separation of diverse anionic analytes on the anion exchangers was demonstrated. • The prepared anion exchangers exhibited high stability.

  12. Hydrophilic interaction chromatography (HILIC) in the analysis of antibiotics.

    Science.gov (United States)

    Kahsay, Getu; Song, Huiying; Van Schepdael, Ann; Cabooter, Deirdre; Adams, Erwin

    2014-01-01

    This paper presents a general overview of the application of hydrophilic interaction chromatography (HILIC) in the analysis of antibiotics in different sample matrices including pharmaceutical, plasma, serum, fermentation broths, environmental water, animal origin, plant origin, etc. Specific applications of HILIC for analysis of aminoglycosides, β-lactams, tetracyclines and other antibiotics are reviewed. HILIC can be used as a valuable alternative LC mode for separating small polar compounds. Polar samples usually show good solubility in the mobile phase containing some water used in HILIC, which overcomes the drawbacks of the poor solubility often encountered in normal phase LC. HILIC is suitable for analyzing compounds in complex systems that elute near the void in reversed-phase chromatography. Ion-pair reagents are not required in HILIC which makes it convenient to couple with MS hence its increased popularity in recent years. In this review, the retention mechanism in HILIC is briefly discussed and a list of important applications is provided including main experimental conditions and a brief summary of the results. The references provide a comprehensive overview and insight into the application of HILIC in antibiotics analysis. Copyright © 2013 Elsevier B.V. All rights reserved.

  13. Bone protein extraction without demineralization using principles from hydroxyapatite chromatography.

    Science.gov (United States)

    Cleland, Timothy P; Vashishth, Deepak

    2015-03-01

    Historically, extraction of bone proteins has relied on the use of demineralization to better retrieve proteins from the extracellular matrix; however, demineralization can be a slow process that restricts subsequent analysis of the samples. Here, we developed a novel protein extraction method that does not use demineralization but instead uses a methodology from hydroxyapatite chromatography where high concentrations of ammonium phosphate and ammonium bicarbonate are used to extract bone proteins. We report that this method has a higher yield than those with previously published small-scale extant bone extractions, with and without demineralization. Furthermore, after digestion with trypsin and subsequent high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) analysis, we were able to detect several extracellular matrix and vascular proteins in addition to collagen I and osteocalcin. Our new method has the potential to isolate proteins within a short period (4h) and provide information about bone proteins that may be lost during demineralization or with the use of denaturing agents. Copyright © 2014 Elsevier Inc. All rights reserved.

  14. Modern Aspects Of Fluorometric Detection In Liquid-Phase Chromatography

    Science.gov (United States)

    Bousquet, Bernard; Garnier, Jean P.; Dreux, Claude

    1983-10-01

    Recent advances are described in the combined use of fluorometric derivatization and high performance liquid chromatography (HPLC) for clinical chemistry determinations. Derivatives (especially dansyl derivatives) can be formed prior to chromatography in the case of estrogens, amino acids, and catecholamines. In post-column reactions, we preferred to use air-segmented reactions as they conform better to all the optimized chromatographic and spectrofluorometric parameters. Fluorescent derivatives produced from cate-cholamines, tryptophan and its metabolites, hydroxyindoles, tryptamine, amino acids, sugars, polyamines, and other substances are often sufficiently sensitive to be detected in picogram quantities by HPLC. Their reaction principle and some of their applications to samples are described. Recently, chemical excitation of fluorophore-like dansyl amino acid was proposed as a detection system for HPLC. By a post-column reaction, a fluorophore can be made to emit light by its reaction with trichlorophenyl oxalate (TCPO) and hydrogen peroxide. The detection limit of this system is about 10 fmol for each dansyl amino acid. Application of this new reaction to catecholamines opens up new prospects for fluorometric detection.

  15. Group type analysis of asphalt by column liquid chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, C.; Yang, J.; Xue, Y.; Li, Y. [Chinese Academy of Science, Taiyuan (China)

    2008-07-01

    An improved analysis method for characterization of asphalt was established. The method is based on column chromatography technique. The asphalts were separated into four groups: saturates, aromatics, resins, and asphaltenes, quantitatively. About 0.1 g of sample was required in each analysis. About 20 mL of n-heptanes was used to separate out saturates first. Then about 35 mL of n-heptanes/dichloromethane (.5, v/v) mixture was used to separate out aromatics. About 30 mL of dichloromethane/tetrahydrofuran (1/3, v/v) mixture was used to separate out resin. The quality of the separation was confirmed by infrared spectra (IR) and {sup 1}H NMR analysis. The model compounds, tetracosan for saturates, dibenz(o)anthracen for aromatics, and acetanilide for resins were used for verification. The IR and {sup 1}H NMR analysis of the prepared fractions from the column liquid chromatography were in good agreement that of pure reagents.

  16. Temperature-responsive chromatography for the separation of biomolecules.

    Science.gov (United States)

    Kanazawa, Hideko; Okano, Teruo

    2011-12-09

    Temperature-responsive chromatography for the separation of biomolecules utilizing poly(N-isopropylacrylamide) (PNIPAAm) and its copolymer-modified stationary phase is performed with an aqueous mobile phase without using organic solvent. The surface properties and function of the stationary phase are controlled by external temperature changes without changing the mobile-phase composition. This analytical system is based on nonspecific adsorption by the reversible transition of a hydrophilic-hydrophobic PNIPAAm-grafted surface. The driving force for retention is hydrophobic interaction between the solute molecules and the hydrophobized polymer chains on the stationary phase surface. The separation of the biomolecules, such as nucleotides and proteins was achieved by a dual temperature- and pH-responsive chromatography system. The electrostatic and hydrophobic interactions could be modulated simultaneously with the temperature in an aqueous mobile phase, thus the separation system would have potential applications in the separation of biomolecules. Additionally, chromatographic matrices prepared by a surface-initiated atom transfer radical polymerization (ATRP) exhibit a strong interaction with analytes, because the polymerization procedure forms a densely packed polymer, called a polymer brush, on the surfaces. The copolymer brush grafted surfaces prepared by ATRP was an effective tool for separating basic biomolecules by modulating the electrostatic and hydrophobic interactions. Applications of thermally responsive columns for the separations of biomolecules are reviewed here. Copyright © 2011 Elsevier B.V. All rights reserved.

  17. Protein separations using enhanced-fluidity liquid chromatography.

    Science.gov (United States)

    Bennett, Raffeal; Olesik, Susan V

    2017-11-10

    Enhanced-fluidity liquid chromatography (EFLC) methods using methanol/H 2 O/CO 2 and hydrophilic interaction liquid chromatography (HILIC) were explored for the separation of proteins and peptides. EFLC is a separation mode that uses a mobile phase made of conventional solvents combined with liquid carbon dioxide (CO 2 ) in subcritical conditions. The addition of liquid CO 2 enhances diffusivity and decreases viscosity while maintaining mixture polarity, which typically results in reduced time of analysis. TFA additive and elevated temperature were leveraged as key factors in the separation of a 13-analyte intact protein mixture in under 5min. Under these conditions EFLC showed modest improvement in terms of peak asymmetry and analysis time over the competing ACN/H 2 O separation. Protein analytes detected by electrospray ionization - quadrupole time of flight, were shown to be unaffected by the addition of CO 2 in the mobile phase. Herein, the feasibility of separating hydrophilic proteins up to 80kDa (with transferrin) is demonstrated for CO 2 -containing mobile phases. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Purification of bacteriophage M13 by anion exchange chromatography.

    Science.gov (United States)

    Monjezi, Razieh; Tey, Beng Ti; Sieo, Chin Chin; Tan, Wen Siang

    2010-07-01

    M13 is a non-lytic filamentous bacteriophage (phage). It has been used widely in phage display technology for displaying foreign peptides, and also for studying macromolecule structures and interactions. Traditionally, this phage has been purified by cesium chloride (CsCl) density gradient ultracentrifugation which is highly laborious and time consuming. In the present study, a simple, rapid and efficient method for the purification of M13 based on anion exchange chromatography was established. A pre-packed SepFast Super Q column connected to a fast protein liquid chromatography (FPLC) system was employed to capture released phages in clarified Escherichia coli fermented broth. An average yield of 74% was obtained from a packed bed mode elution using citrate buffer (pH 4), containing 1.5 M NaCl at 1 ml/min flow rate. The purification process was shortened substantially to less than 2 h from 18 h in the conventional ultracentrifugation method. SDS-PAGE revealed that the purity of particles was comparable to that of CsCl gradient density ultracentrifugation method. Plaque forming assay showed that the purified phages were still infectious. Copyright 2010 Elsevier B.V. All rights reserved.

  19. Supercritical Fluid Chromatography- A Hybrid of GC and LC

    Directory of Open Access Journals (Sweden)

    Kaushal K Chandrul

    2010-03-01

    Full Text Available

    High performance specifications and unique functionality of chromatographic techniques is a demand of pharmaceutical industry and research. This leads to the origin of Supercritical Fluid Chromatography (SFC. It is a rapidly expanding analytical technique. The main feature that differentiates SFC from other chromatographic techniques is the replacement of either the liquid or gas mobile phase with a supercritical fluid mobile phase. It is considered a hybrid of GC and LC technique. High diffusion coefficient and low viscosity of supercritical fluids is responsible for high speed analysis, high efficiency and high sensitivity. Low mobile-phase flow rate, density programming and compatability with GC and LC detectors make SFC a versatile chromatographic technique in analytical research and development. It has a unique characteristic of analyzing thermo labile or non-volatile substances. This review highlights the role of supercritical fluid chromatography in the separation of polymers, thermally labile pesticides, fatty acids, metal chelates and organometallic compounds, chiral and achiral molecules, identification and analysis of polar samples, explosives, drugs of abuse and application of SFC in forensic science (fingerprinting. 

  20. Supercritical fluid chromatography-A Hybrid of GC and LC

    Directory of Open Access Journals (Sweden)

    Neha Sethi

    2010-01-01

    Full Text Available High performance specifications and unique functionality of chromatographic techniques is a demand of pharmaceutical industry and research. This leads to the origin of Supercritical Fluid Chromatography (SFC. It is a rapidly expanding analytical technique. The main feature that differentiates SFC from other chromatographic techniques is the replacement of either the liquid or gas mobile phase with a supercritical fluid mobile phase. It is considered a hybrid of GC and LC technique. High diffusion coefficient and low viscosity of supercritical fluids is responsible for high speed analysis, high efficiency and high sensitivity. Low mobile-phase flow rate, density programming and compatability with GC and LC detectors make SFC a versatile chromatographic technique in analytical re-search and development. It has a unique characteristic of analyzing thermo labile or non-volatile substances. This review highlights the role of supercritical fluid chromatography in the separation of polymers, thermally labile pesticides, fatty acids, metal chelates and organometallic compounds, chiral and achiral molecules, identification and analysis of polar samples, explosives, drugs of abuse and application of SFC in forensic science (fingerprint-ing.

  1. Pushing the speed limit in enantioselective supercritical fluid chromatography.

    Science.gov (United States)

    Regalado, Erik L; Welch, Christopher J

    2015-08-01

    Chromatographic enantioseparations on the order of a few seconds can be achieved by supercritical fluid chromatography using short columns packed with chiral stationary phases. The evolution of 'world record' speeds for the chromatographic separation of enantiomers has steadily dropped from an industry standard of 20-40 min just two decades ago, to a current ability to perform many enantioseparations in well under a minute. Improvements in instrument and column technologies enabled this revolution, but the ability to predict optimal separation time from an initial method development screening assay using the t(min cc) predictor greatly simplifies the development and optimization of high-speed chiral chromatographic separations. In this study, we illustrate how the use of this simple tool in combination with the workhorse technique of supercritical fluid chromatography on customized short chiral columns (1-2 cm length) allows us to achieve ultrafast enantioseparations of pharmaceutically relevant compounds on the 5-20 s scale, bringing the technique of high-throughput enantiopurity analysis out of the specialist realm and into the laboratories of most researchers. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  2. Characterization of the fermentation process by gas chromatography Lasiodiplodia theobromae and gas chromatography coupled with mass spectrometry

    International Nuclear Information System (INIS)

    Castillo Portela, Grolamys; Eng Sanchez, Felipe; Nogueiras Lima, Clara

    2014-01-01

    Lasiodiplodia theobromae is a fungus, which has been reported by some authors as a high yield producer of the phytohormone jasmonic acid (JA). An indigenous strain of this fungus has been used for producing a fermentation broth with a high JA concentration by the Cuban Research Institute for Sugar Cane Derivatives (ICIDCA), registered as BIOJAS. The broth has been applied to some agricultural crops and demonstrated its economic feasibility as plant growth regulator and biological control of various phytopathogenic microorganisms and pests. Both fermentation broth and biomass from this fungus contain some other metabolites having bioactive properties, for instance, fatty acids. This paper shows the composition and quantification of fatty acids in the biomass using Gas Chromatography (GC) and the identification of substances profile in fermentation broth by Gas Chromatography coupled to Mass Spectrometry (GC-MS). The most fatty acids in the biomass are palmitic, stearic, oleic, linoleic and linolenic acids, being oleic acid the major component. On the other hand, 2,32 % of fatty acid esters; 2,47 % of alkenes; 14,40 % of alcohols; 30,15 % of aldehydes and 21,73 % of paraffins were detected in the composition of fermentation broth

  3. Phytochemical Profile of Erythrina variegata by Using High-Performance Liquid Chromatography and Gas Chromatography-Mass Spectroscopy Analyses.

    Science.gov (United States)

    Muthukrishnan, Suriyavathana; Palanisamy, Subha; Subramanian, Senthilkumar; Selvaraj, Sumathi; Mari, Kavitha Rani; Kuppulingam, Ramalingam

    2016-08-01

    Natural products derived from plant sources have been utilized to treat patients with numerous diseases. The phytochemical constituents present in ethanolic leaf extract of Erythrina variegata (ELEV) were identified by using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectroscopy (GC-MS) analyses. Shade dried leaves were powdered and extracted with ethanol for analyses through HPLC to identify selected flavonoids and through GC-MS to identify other molecules. The HPLC analysis of ELEV showed the presence of gallic and caffeic acids as the major components at concentrations of 2.0 ppm and 0.1 ppm, respectively, as well as other components. GC-MS analysis revealed the presence of 3-eicosyne; 3,7,11,15-tetramethyl-2-hexadecen-1-ol; butanoic acid, 3-methyl-3,7-dimethyl-6-octenyl ester; phytol; 1,2-benzenedicarboxylic acid, diundecyl ester; 1-octanol, 2-butyl-; squalene; and 2H-pyran, 2-(7-heptadecynyloxy) tetrahydro-derivative. Because pharmacopuncture is a new evolving natural mode that uses herbal extracts for treating patients with various ailments with minimum pain and maximum effect, the results of this study are particularly important and show that ELEV possesses a wide range of phytochemical constituents, as indicated above, as effective active principle molecules that can be used individually or in combination to treat patients with various diseases. Copyright © 2016. Published by Elsevier B.V.

  4. Gas chromatography-mass spectrometry and high-performance liquid chromatography-diode array detection for dating of paper ink.

    Science.gov (United States)

    Díaz-Santana, Oscar; Vega-Moreno, Daura; Conde-Hardisson, Francisco

    2017-09-15

    An extraction and determination method is shown for the analysis of dyes and solvents present in two types of ballpoint pen inks that are deposited onto paper. Ink extracts are analysed using a combination of gas chromatography with mass spectrometry (GC-MS), and high-pressure liquid chromatography with photodiode array detection (HPLC-DAD), within a single sample extraction procedure. Seventeen solvents and thirteen dyes contained in two Montblanc ® inks (black and blue) were monitored for 45 months at monthly intervals, in order to determine variations in the concentrations of the compounds over time. We also studied the relative variations between different compounds and the generation of degradation products such as phenol. The concentration data obtained from these compounds during their exposure have been analysed and a multiple regression model is developed for each ink type that allows an estimate of the exposure time of the ink on paper with a maximum error of between 4 and 7 months. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Pressurized planar electrochromatography, high-performance thin-layer chromatography and high-performance liquid chromatography--comparison of performance.

    Science.gov (United States)

    Płocharz, Paweł; Klimek-Turek, Anna; Dzido, Tadeusz H

    2010-07-16

    Kinetic performance, measured by plate height, of High-Performance Thin-Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Pressurized Planar Electrochromatography (PPEC) was compared for the systems with adsorbent of the HPTLC RP18W plate from Merck as the stationary phase and the mobile phase composed of acetonitrile and buffer solution. The HPLC column was packed with the adsorbent, which was scrapped from the chromatographic plate mentioned. An additional HPLC column was also packed with adsorbent of 5 microm particle diameter, C18 type silica based (LiChrosorb RP-18 from Merck). The dependence of plate height of both HPLC and PPEC separating systems on flow velocity of the mobile phase and on migration distance of the mobile phase in TLC system was presented applying test solute (prednisolone succinate). The highest performance, amongst systems investigated, was obtained for the PPEC system. The separation efficiency of the systems investigated in the paper was additionally confirmed by the separation of test component mixture composed of six hormones. 2010 Elsevier B.V. All rights reserved.

  6. Evaluation between ultrahigh pressure liquid chromatography and high-performance liquid chromatography analytical methods for characterizing natural dyestuffs.

    Science.gov (United States)

    Serrano, Ana; van Bommel, Maarten; Hallett, Jessica

    2013-11-29

    An evaluation was undertaken of ultrahigh pressure liquid chromatography (UHPLC) in comparison to high-performance liquid chromatography (HPLC) for characterizing natural dyes in cultural heritage objects. A new UHPLC method was optimized by testing several analytical parameters adapted from prior UHPLC studies developed in diverse fields of research. Different gradient elution programs were tested on seven UHPLC columns with different dimensions and stationary phase compositions by applying several mobile phases, flow rates, temperatures, and runtimes. The UHPLC method successfully provided more improved data than that achieved by the HPLC method. Indeed, even though carminic acid has shown circa 146% higher resolution with HPLC, UHPLC resulted in an increase of 41-61% resolution and a decrease of 91-422% limit of detection, depending on the dye compound. The optimized method was subsequently assigned to analyse 59 natural reference materials, in which 85 different components were ascribed with different physicochemical properties, in order to create a spectral database for future characterization of dyes in cultural heritage objects. The majority of these reference samples could be successfully distinguished with one single method through the examination of these compounds' retention times and their spectra acquired with a photodiode array detector. These results demonstrate that UHPLC analyses are extremely valuable for the acquisition of more precise chromatographic information concerning natural dyes with complex mixtures of different and/or closely related physicochemical properties, essential for distinguishing similar species of plants and animals used to colour cultural heritage objects. Copyright © 2013 Elsevier B.V. All rights reserved.

  7. Supercritical fluid chromatography hyphenated with twin comprehensive two-dimensional gas chromatography for ultimate analysis of middle distillates.

    Science.gov (United States)

    Adam, Frédérick; Thiébaut, Didier; Bertoncini, Fabrice; Courtiade, Marion; Hennion, Marie-Claire

    2010-02-19

    This paper reports the conditions of online hyphenation of supercritical fluid chromatography (SFC) with twin comprehensive two-dimensional gas chromatography (twin-GCxGC) for detailed characterization of middle distillates; this is essential for a better understanding of reactions involved in refining processes. In this configuration, saturated and unsaturated compounds that have been fractionated by SFC are transferred on two different GC x GC columns sets (twin-GCxGC) placed in the same GC oven. Cryogenic focusing is used for transfer of fractions into the first dimension columns before simultaneous GCxGC analysis of both saturated and unsaturated fractions. The benefits of SFC-twin-GC x GC are demonstrated for the extended alkane, iso-alkane, alkene, naphthenes and aromatics analysis (so-called PIONA analysis) of diesel samples which can be achieved in one single injection. For that purpose, saturated and unsaturated compounds have been separated by SFC using a silver loaded silica column prior to GC x GC analysis. Alkenes and naphthenes are quantitatively recovered in the unsaturated and saturated fractions, respectively, allowing their identification in various diesel samples. Thus, resolution between each class of compounds is significantly improved compared to a single GCxGC run, and for the first time, an extended PIONA analysis of diesel samples is presented. Copyright 2009 Elsevier B.V. All rights reserved.

  8. Simultaneous achiral-chiral analysis of pharmaceutical compounds using two-dimensional reversed phase liquid chromatography-supercritical fluid chromatography.

    Science.gov (United States)

    Venkatramani, C J; Al-Sayah, Mohammad; Li, Guannan; Goel, Meenakshi; Girotti, James; Zang, Lisa; Wigman, Larry; Yehl, Peter; Chetwyn, Nik

    2016-02-01

    A new interface was designed to enable the coupling of reversed phase liquid chromatography (RPLC) and supercritical fluid chromatography (SFC). This online two-dimensional chromatographic system utilizing RPLC in the first dimension and SFC in the second was developed to achieve simultaneous achiral and chiral analysis of pharmaceutical compounds. The interface consists of an eight-port, dual-position switching valve with small volume C-18 trapping columns. The peaks of interest eluting from the first RPLC dimension column were effectively focused as sharp concentration pulses on small volume C-18 trapping column/s and then injected onto the second dimension SFC column. The first dimension RPLC separation provides the achiral purity result, and the second dimension SFC separation provides the chiral purity result (enantiomeric excess). The results are quantitative enabling simultaneous achiral, chiral analysis of compounds. The interface design and proof of concept demonstration are presented. Additionally, comparative studies to conventional SFC and case studies of the applications of 2D LC-SFC in pharmaceutical analysis is presented. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Too beautiful to be true?; Zu schoen, um wahr zu werden?

    Energy Technology Data Exchange (ETDEWEB)

    Sieg, Klaus

    2013-06-15

    In Kenya, the largest wind farm of Africa should be established. But first, the electricity grid must be developed. Far better used than wind energy is geothermal energy in Kenya. [German] In Kenia soll der groesste Windpark Afrikas entstehen. Doch zunaechst muss das Stromnetz ausgebaut werden. Weit besser genutzt als die Windenergie wird die Geothermie in Kenia.

  10. EU-Verordnung zu "Konfliktmineralien": Ein Schritt zu höherer Rechenschaftspflicht im Rohstoffsektor?

    OpenAIRE

    Küblböck, Karin; Grohs, Hannes

    2017-01-01

    "No blood in my cell phone" - Anfang der 2000er-Jahre zeigten Kampagnen von Nichtregierungsorganisationen (NGOs) Verbindungen zwischen Rohstoffen in Elektronikprodukten und der Finanzierung des Krieges in der Demokratischen Republik Kongo (DRC) auf. In diesem Zusammenhang rückte auch die Verantwortung von Unternehmen für die Gestaltung ihrer Lieferkette verstärkt ins öffentliche Bewusstsein. 2017 tritt nun eine EU-Verordnung in Kraft, die verhindern soll, dass Unternehmen durch ihre Rohstoffb...

  11. Using Aspen to Teach Chromatographic Bioprocessing: A Case Study in Weak Partitioning Chromatography for Biotechnology Applications

    Science.gov (United States)

    Evans, Steven T.; Huang, Xinqun; Cramer, Steven M.

    2010-01-01

    The commercial simulator Aspen Chromatography was employed to study and optimize an important new industrial separation process, weak partitioning chromatography. This case study on antibody purification was implemented in a chromatographic separations course. Parametric simulations were performed to investigate the effect of operating parameters…

  12. Analytical method for high resolution liquid chromatography for quality control French Macaw

    International Nuclear Information System (INIS)

    Garcia Penna, Caridad M; Torres Amaro, Leonid; Menendez Castillo, Rosa; Sanchez, Esther; Martinez Espinosa, Vivian; Gonzalez, Maria Lidia; Rodriguez, Carlos

    2007-01-01

    Was developed and validated an analytical method for high resolution liquid chromatography applicable to quality control of drugs dry French Macaw (Senna alata L. Roxb.) With ultraviolet detection at 340 nm. The method for high resolution liquid chromatography used to quantify the sennosides A and B, main components, was validated and proved to be specific, linear, precise and accurate. (Author)

  13. Surface modification of chromatography adsorbents by low temperature low pressure plasma

    DEFF Research Database (Denmark)

    Arpanaei, Ayyoob; Winther-Jensen, Bjørn; Theodosiou, E.

    2010-01-01

    In this study we show how low temperature glow discharge plasma can be used to prepare bi-layered chromatography adsorbents with non-adsorptive exteriors. The commercial strong anion exchange expanded bed chromatography matrix, Q HyperZ, was treated with plasmas in one of two general ways. Using ...

  14. Identification of Cyclopentenyl Fatty Acids by Gas Liquid Chromatography and Mass Spectrometry

    DEFF Research Database (Denmark)

    Shukla, V. K. S.; Abdel-Moety, E. M.; Larsen, Elfinn

    1979-01-01

    The straight chain fatty acids and the cyclopentenyl fatty acids present in the lipids of Hydnocarpus wightiana seeds were separated as their pyrrolidides by means of gas chromatography. A gas chromatography-mass spectrometry system confirmed the complete separation and permitted the identification...

  15. Determination of basic azaarenes and polynuclear aromatic hydrocarbons in airborne particulate matter by gas chromatography

    DEFF Research Database (Denmark)

    Nielsen, Torben; Clausen, Peraxel; Jensen, Finn Palmgren

    1986-01-01

    phase (adjusted to pH 14 with potassium hydroxide) with dichloromethane, and determined by capillary gas chromatography (g.c.) with a nitrogen-sensitive detector. The PAH in the toluene phase are isolated by means of semipreparative high-performance liquid chromatography and liquid-liquid extraction...

  16. Multidimensional chromatography coupled to mass spectrometry in analysing complex proteomics samples

    NARCIS (Netherlands)

    Horvatovich, Peter; Hoekman, Berend; Govorukhina, Natalia; Bischoff, Rainer

    Multidimensional chromatography coupled to mass spectrometry (LC(n)-MS) provides more separation power and an extended measured dynamic concentration range to analyse complex proteomics samples than one dimensional liquid chromatography coupled to mass spectrometry (1D-LC-MS). This review gives an

  17. A Modern Apparatus for Performing Flash Chromatography: An Experiment for the Organic Laboratory

    Science.gov (United States)

    Naumiec, Gregory R.; Del Padre, Angela N.; Hooper, Matthew M.; Germaine, Alison St.; DeBoef, Brenton

    2013-01-01

    A modern apparatus for performing flash chromatography using commercially available, prepacked silica cartridges has been developed. The key advantage of this system, when compared to traditional flash chromatography, is its use of commercially available silica cartridges, which obviates the need for students to handle silica gel. The apparatus…

  18. Recent advances in ultra-high performance liquid chromatography for the analysis of traditional chinese medicine

    Science.gov (United States)

    Traditional Chinese medicines (TCMs) have been widely used for the prevention and treatment of various diseases for thousands of years in China. Ultra Performance Liquid Chromatography (UHPLC) is a relatively new technique offering new possibilities in liquid chromatography. This paper reviews recen...

  19. Investigating the Retention Mechanisms of Liquid Chromatography Using Solid-Phase Extraction Cartridges

    Science.gov (United States)

    O'Donnell, Mary E.; Musial, Beata A.; Bretz, Stacey Lowery; Danielson, Neil D.; Ca, Diep

    2009-01-01

    Liquid chromatography (LC) experiments for the undergraduate analytical laboratory course often illustrate the application of reversed-phase LC to solve a separation problem, but rarely compare LC retention mechanisms. In addition, a high-performance liquid chromatography instrument may be beyond what some small colleges can purchase. Solid-phase…

  20. A rapid method for the separation and estimation of uranium in geological materials using ion chromatography

    International Nuclear Information System (INIS)

    Prakash, Satya; Bangroo, P.N.

    2013-01-01

    Ion Chromatography is an elegant analytical technique which was primarily developed for the analysis of anionic species and over the years it has been used successfully to analyse various elements in different matrices. In this work the potential of Ion Chromatography has been used for the rapid separation and estimation of uranium in hydrogeochemical and other geological materials