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Sample records for chondrodysplasia punctata

  1. The neurology of rhizomelic chondrodysplasia punctata

    OpenAIRE

    Bams-Mengerink, Annemieke M; Koelman, Johannes HTM; Waterham, Hans; Barth, Peter G; Poll-The, Bwee Tien

    2013-01-01

    Background To describe the neurologic profiles of Rhizomelic chondrodysplasia punctata (RCDP); a peroxisomal disorder clinically characterized by skeletal abnormalities, congenital cataracts, severe growth and developmental impairments and immobility of joints. Defective plasmalogen biosynthesis is the main biochemical feature. Methods Observational study including review of clinical and biochemical abnormalities, genotype, presence of seizures and neurophysiological studies of a cohort of 16...

  2. Genetics Home Reference: X-linked chondrodysplasia punctata 1

    Science.gov (United States)

    Skip to main content Your Guide to Understanding Genetic Conditions Enable Javascript for addthis links to activate. ... Conditions Genes Chromosomes & mtDNA Resources Help Me Understand Genetics Home Health Conditions X-linked chondrodysplasia punctata 1 ...

  3. Brachytelephalangic chondrodysplasia punctata: prenatal diagnosis and postnatal outcome.

    OpenAIRE

    Boulet, Stéphanie; Dieterich, Klaus; Althuser, Marc; Nugues, Frédérique; Durand, Claudia; Charra, Catherine; Schaal, Jean-Patrick; Jouk, Pierre-Simon

    2010-01-01

    International audience We report the prenatal management of a brachytelephalangic chondrodysplasia punctata (CDPX1) case and how postnatal findings confirmed the diagnosis. The mother was initially referred after ultrasound revealed an abnormal fetal mid-face and punctuation of upper femoral epiphyses. Chondrodysplasia punctata (CP) with Binder anomaly was suspected. 3D-HCT revealed brachytelephalangy suggesting CDPX1. At birth, mid-face hypoplasia was marked. Postnatal imaging and genetic...

  4. Rare Case of Rhizomelic Chondrodysplasia Punctata

    Science.gov (United States)

    Mahale, Yashwant; Kadu, Vikram V.; Chaudhari, Amit

    2015-01-01

    Introduction: Rhizomelic chondrodysplasia punctata (RCDP) is a very rare disease. It impairs the normal development of many parts of the body. The features of this disorder include bony abnormalities, severe mental retardation, joint contractures, cataract and recurrent respiratory infections and breathing problems. Seizures and Distinctive facial features including prominent forehead, depressed nasal bridge and small nose is also associated with this pathology. Being rare, this is very difficult to diagnose when presented at OPD. Proper history and meticulous examination is extremely necessary. Our aim is to discuss current knowledge on etiopathogenesis as well as radiological and clinical symptoms of diseases associated with RCDP. Case Report: 5 yrs old male child presented with chest infection and periarticular swelling of all the small and large joints. The patient was walking with limp. History elicited that the child was born of a consanguineous marriage. The child was delivered at home. Birth weight was 2.4 kgs. He repeatedly had upper respiratory tract infections and was taking treatment for the same. He was further investigated in the form of clinical, biochemical and radiological assessment which stated that the patient was suffering from RCDP. Conclusion: This is a rare presentation. Though this is not curable, management of RCDP is symptomatic and supportive and may include physiotherapy and orthopedic procedures (in later stages) to improve function. The child may also undergo cataract surgery to improve vision. PMID:27299065

  5. [Symptomatic calcification in the newborn. Phenocopies of chondrodysplasia punctata].

    Science.gov (United States)

    Leicher-Düber, A; Schumacher, R; Spranger, J

    1990-04-01

    Stippled epiphyses occur in the new-born and young infant in the different hereditary forms of chondrodysplasia punctata. Symptomatic stippling has been described also in association with chromosomal anomalies, gangliosidosis and drug induced embryopathies. We present patients with Cumarin-embryopathy (2), fetal alcohol syndrome (1), Zellweger-syndrome (2) and chromosomal anomaly 16 (1) and discuss the typical roentgenographic features, distribution and differential diagnosis of epiphyseal stippling. PMID:2160110

  6. Severe tracheobronchial stenosis and cervical vertebral subluxation in X-linked recessive chondrodysplasia punctata

    Energy Technology Data Exchange (ETDEWEB)

    Mundinger, Gerhard S. [Johns Hopkins Hospital, Division of Plastic, Reconstructive, and Maxillofacial Surgery, Baltimore, MD (United States); Weiss, Clifford; Fishman, Elliot K. [Johns Hopkins Hospital, Russell H. Morgan Department of Radiology and Radiological Science, Baltimore, MD (United States)

    2009-06-15

    Radiologic manifestations of X-linked chondrodysplasia punctata (CDPX1) typically include chondrodysplasia, epiphyseal stippling, punctate calcification of cartilage, distal phalangeal hypoplasia, and nasal/midface hypoplasia. We present an infant with CDPX1 demonstrating calcification and stenosis of the entire trachea and mainstem bronchi, as well as possible anterior C1 subluxation due to progression of congenital vertebral dysplasia. (orig.)

  7. Neonatal lupus syndrome: a case with chondrodysplasia punctata and other unusual manifestations.

    OpenAIRE

    Austin-Ward, E; Castillo, S.; Cuchacovich, M; Espinoza, A; Cofré-Beca, J; González, S; Solivelles, X; Bloomfield, J.

    1998-01-01

    We report a case of a newborn infant whose mother had systemic lupus erythematosus (SLE) diagnosed before pregnancy. The child had clinical manifestations of neonatal lupus as well as chondrodysplasia punctata and other findings that resemble the congenital anomalies associated with the use of oral anticoagulants, with no history of exposure. We speculate that the combined action of the different maternal autoantibodies may produce the whole spectrum of manifestations.

  8. Systemic lupus erythematosus in pregnancy with rare anomaly of rhizomelic chondrodysplasia punctata in baby

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    Alakananda

    2016-06-01

    Full Text Available Rhizomelic means shortening of the bones closest to the body's trunk. Chondrodysplasia refers to malformation (the dysplasia part of the word of the cartilage (the chondro part of the word. Punctata refers to an unusual stippling on the end of the bones that join the shoulders and elbows (the humerus and the top of the leg and the hip (femur. On X-ray an infant's bones look spotty at the ends. Here, we report a case of rhizomelic chondrodysplasia punctate (RCDP in newborn of a known systemic lupus erythematosus (SLE patient. Consent for publication of this rare case for academic benefit has been taken from the patient. [Int J Res Med Sci 2016; 4(6.000: 2461-2463

  9. Clinical and radiological pictures of two newborn babies with manifestations of chondrodysplasia punctata and review of available literature

    International Nuclear Information System (INIS)

    Chondrodysplasia punctata (CDP) is a rare, heterogeneous congenital skeletal dysplasia, characterized by punctate or dot-like calcium deposits in cartilage observed on neonatal radiograms. A number of inborn metabolic diseases are associated with CDP, including peroxisomal and cholesterol biosynthesis dysfunction and other inborn errors of metabolism such as: mucolipidosis type II, mucopolysacharidosis type III, GM1 gangliosidosis. CDP is also related to disruption of vitamin K-dependent metabolism, causing secondary effects on the embryo, as well as fetal alcohol syndrome (FAS), chromosomal abnormalities that include trisomies 18 and 21, Turner syndrome. This article presents clinical data and diagnostic imaging findings of two newborn babies with chondrodysplasia punctata. Children presented with skeletal and cartilage anomalies, dysmorphic facial feature, muscles tone abnormalities, skin changes and breathing difficulties. One of the patients demonstrated critical stenosis of spinal canal with anterior subluxation of C1 vertebra relative to C2. The aim of this article is to present cases and briefly describe current knowledge on etiopathogenesis as well as radiological and clinical symptoms of diseases coexisting with CDP. Radiological diagnostic imaging allows for visualization of punctate focal mineralization in bone epiphyses during neonatal age and infancy. Determining the etiology of chondrodysplasia punctata requires performing various basic as well as additional examinations, including genetic studies

  10. Condrodisplasia punctata rizomélica: relato de caso e breve revisão da literatura Rhizomelic chondrodysplasia punctata: a case report and brief literature review

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    Sizenildo da Silva Figueirêdo

    2007-02-01

    Full Text Available Apresentamos um caso de uma lactente de dois meses de idade acometida pela forma recessiva da condrodisplasia punctata, doença caracterizada, radiologicamente, por acentuado encurtamento proximal e distúrbio de ossificação (epífises puntiformes dos membros. São enfatizados os achados clínico-radiológicos, bem como seus principais diagnósticos diferenciais, baseados em dados de breve revisão da literatura.The authors present a case of a 2-month-old infant affected by the recessive form of chondrodysplasia punctata, a rare condition radiologically characterized by severe proximal shortening and anomalous ossification (epiphyseal stippling of the limbs. Clinical and radiological findings as well as main differential diagnoses are emphasized on the basis of data originating from a brief literature review.

  11. Prenatal diagnosis of chondrodysplasia punctata tibia-metacarpal type using multidetector CT and three-dimensional reconstruction

    Energy Technology Data Exchange (ETDEWEB)

    Miyazaki, Osamu [National Centre for Child Health and Development, Department of Radiology, Tokyo (Japan); Nishimura, Gen [Tokyo Metropolitan Kiyose Children' s Hospital, Department of Radiology, Tokyo (Japan); Sago, Haruhiko; Watanabe, Noriyoshi; Ebina, Shunsuke [National Centre for Child Health and Development, Department of Perinatal Medicine and Maternal Care, Tokyo (Japan)

    2007-11-15

    We report a case of chondrodysplasia punctata tibia-metacarpal type (CDP-TM) that was diagnosed prenatally using multidetector CT (MDCT) with three-dimensional (3-D) CT reconstructions. Prenatal US had shown severe thoracic hypoplasia and rhizomelic shortening of the limbs, raising the suspicion of thanatophoric dysplasia. However, MDCT showed punctate calcifications in the epiphyseal cartilage of the humeri and femora, carpal bones, and paravertebral region. On 3-D CT, the tibiae were much shorter than the fibulae, the humeri were very short and bowed, and severe platyspondyly was evident. These findings led to the diagnosis of CDP-TM. The diagnosis was confirmed on postnatal radiographs. Prenatal MDCT with 3-D images may make a useful contribution to prenatal diagnosis in selected fetuses with severe skeletal dysplasia. (orig.)

  12. Whole Exome Sequencing Reveals Compound Heterozygosity for Ethnically Distinct PEX7 Mutations Responsible for Rhizomelic Chondrodysplasia Punctata, Type 1

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    Jessie C. Jacobsen

    2015-01-01

    Full Text Available We describe two brothers who presented at birth with bone growth abnormalities, followed by development of increasingly severe intellectual and physical disability, growth restriction, epilepsy, and cerebellar and brain stem atrophy, but normal ocular phenotypes. Case 1 died at 19 years of age due to chronic respiratory illnesses without a unifying diagnosis. The brother remains alive but severely disabled at 19 years of age. Whole exome sequencing identified compound heterozygous stop mutations in the peroxisome biogenesis factor 7 gene in both individuals. Mutations in this gene cause rhizomelic chondrodysplasia punctata, type 1 (RCDP1. One mutation, p.Arg232∗, has only been documented once before in a Japanese family, which is of interest given these two boys are of European descent. The other mutation, p.Leu292∗, is found in approximately 50% of RCDP1 patients. These are the first cases of RCDP1 that describe the coinheritance of the p.Arg232∗ and p.Leu292∗ mutations and demonstrate the utility of WES in cases with unclear diagnoses.

  13. Detection of Gene Deletions in Children with Chondrodysplasia Punctata, Ichthyosis, Kallmann Syndrome, and Ocular Albinism by FISH Studies

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    Jia-Woei Hou

    2005-09-01

    Full Text Available Background: Contiguous gene syndrome (CGS is characterized by a series of clinical featuresresulting from interstitial or terminal deletions of various adjacentgenes. Several important genes have been identified in the Xp22.3 region tobe responsible for genetically heterogeneous diseases. In this study, fluorescencein situ hybridization (FISH methods were used to detect the extent ofgene deletion related to the phenotypes of patients with Xp-CGS.Methods: The molecular cytogenetic statuses of 23 boys with at least 1 apparent featureof chondrodysplasia punctata (CDP, ichthyosis, Kallmann syndrome, ortype 1 ocular albinism and those of their family members were investigated.High-resolution banding and FISH studies were performed using the probesof steroid sulfatase (STS, KAL1 and OA1, to detect the deleted status onXp22.3 in these patients along with their mothers and/or sisters or maternalgrandmothers.Results: All of these boys had normal karyotypes. FISH study showed nullisomy in 9of the 23 male patients and hemizygosity in all female carriers in the geneson Xp22.3. The existence of 2 or more diseases in the same individual indicatesa CGS. In addition, a putative mental retardation-related gene onXp22.3 locus was considered to be located between X-linked CDP and STS.Conclusions: The use of FISH probes for the Xp22.3 region allowed us to identify XlinkedCGSs, especially in those patients with 2 or more distinct clinical entitiesor an obvious X-linked disorder.

  14. Genetics Home Reference: X-linked chondrodysplasia punctata 2

    Science.gov (United States)

    ... come from animals (particularly egg yolks, meat, poultry, fish, and dairy products). Although too much cholesterol is a risk factor for heart disease, this molecule is necessary for normal embryonic development and has important functions both before and after ...

  15. Clinical genetics and pathobiology of ciliary chondrodysplasias

    OpenAIRE

    Schmidts, M.

    2014-01-01

    Ciliary chondrodysplasias represent a heterogenous group of rare, nearly exclusively autosomal recessively inherited developmental conditions. While the skeletal phenotype, mainly affecting limbs, ribs and sometimes the craniofacial skeleton, is predominant, extraskeletal disease affecting the kidneys, liver, heart, eyes and other organs and tissues is observed inconsistently. Significant lethality, resulting from cardiorespiratory failure due to thoracic constriction as well as from renal an...

  16. Leukoderma punctata following topical PUVAsol treatment.

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    Fernandes, Nurimar Conceição; Pina, Juliana Carnevale

    2010-01-01

    Two adolescent females and a girl, all with clinically diagnosed vitiligo, were treated with 0.2% 8-methoxypsoralen cream followed by exposure to solar ultraviolet light. One year later, they developed hypopigmented and achromic spots on the areas affected by the vitiligo. Biopsy of skin tissue taken from one of these cases showed a marked reduction in melanin. Clinical and histological findings point to a diagnosis of leukoderma punctata. PMID:20944925

  17. A New Acylated Flavonoid from Anaphalis aureo-punctata

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A new acylated tlavonoid glycoside, 3-O-kaempferol-3-O-acetyl-6-O-(P-coumaroyl)-β-D-glucopyranoside 1 was isolated from the whole plant of Anaphalis aureo-punctata. The structure was established by spectral methods.

  18. Phenolic compounds from Anaphalis aureo-punctata

    Institute of Scientific and Technical Information of China (English)

    WU Yan-Qi; LOU Ning; LI Yu

    2003-01-01

    @@ From the ethanolic extract of the whole plant of Anaphalis aureo-punctata, a new acylated flavonoid glycoside 3-O-kaempferol-3-O-acetyl-6-O-(p-coumaroyl)-β-D-glucopyranoside (1), and five known phenolic compounds 3-O-kaempferol-6- O- ( p-coumaroyl )-β-D-glucopyranoside ( 2 ), kaempferol-3- O-β-D-glucopyranoside 3,6-(4′-hydroxystyryl)-4-methoxy-2-pyrone (4), 2H-pyran-2-one, 6-[ 2-( 4-(β-D-glucopyranosyloxy ) phenyl ) ethenyl ]-4-methoxy-( E ) (5) and 4-hydroxy-3-methoxycinnamic hexacosyl ester (6) were isolated. Their structures were established by spectral methods (UV, IR, MS, 1D, 2D-NMR). The flavonoid glycosides, 1, 2 and 3 showed markedly inhibited oxidative DNA strand breaks induced by Fenton reaction and NADH/PMS in a concentration-dependent manner.

  19. Carex punctata Gaudin (Cyperaceae), nova vrsta u hrvatskoj flori

    OpenAIRE

    Koopman, Jacob; Topić, Jasenka

    2011-01-01

    Carex punctata Gaudin 1811. Agrost. Helv., 2: 152 (Cyperaceae) pronađen je na tri lokaliteta u razdoblju 2004-2009. Vrsta nikad nije zabilježena za Hrvatsku. Pronašao ju je drugi autor, a prvi je potvrdio determinaciju.

  20. Antibacterial activity of Zuccagnia punctata Cav. ethanolic extracts.

    Science.gov (United States)

    Zampini, Iris C; Vattuone, Marta A; Isla, Maria I

    2005-12-01

    The present study was conducted to investigate antibacterial activity of Zuccagnia punctata ethanolic extract against 47 strains of antibiotic-resistant Gram-negative bacteria and to identify bioactive compounds. Inhibition of bacterial growth was investigated using agar diffusion, agar macrodilution, broth microdilution and bioautographic methods. Zuccagnia punctata extract was active against all assayed bacteria (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter cloacae, Serratia marcescens, Morganella morganii, Acinetobacter baumannii, Pseudomonas aeruginosa, Stenotrophomonas maltophilia) with minimal inhibitory concentration (MIC) values ranging from 25 to 200 microg/mL. Minimal bactericidal concentration (MBC) values were identical or two-fold higher than the corresponding MIC values. Contact bioautography, indicated that Zuccagnia punctata extracts possess one major antibacterial component against Pseudomonas aeruginosa and at least three components against. Klebsiella pneumoniae and Escherichia coli. Activity-guided fractionation of 1he ethanol extract on a silica gel column yielded a compound (2',4'-dihydroxychalcone), which exhibited strong antibacterial activity with MIC values between 0.10 and 1.00 microg/mL for Proteus mirabilis, Enterobacter cloacae, Serratia marcescens, Morganella morganii, Acinetobacter baumannii, Pseudomonas aeruginosa, Stenotrophomonas maltophilia. These values are lower than imipenem (0.25-16 microg/mL). Zuccagnia punctata might provide promising therapeutic agents against infections with multi-resistant Gram-negative bacteria. PMID:16137849

  1. Zuccagnia punctata (Leguminosae): ¿nuevo o viejo endemismo argentino?

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    Emilio A. Ulibarri

    2005-01-01

    El estudio bibliográfico y de colecciones botánicas pertenecientes a Zuccagnia punctata demostraron el caracter endémico de esta especie para la Argentina. Con la descripción, ilustración y otros datos complementarios, se brinda un panorama que enriquece el concepto sobre esta interesante especie.

  2. Effects of Zuccagnia punctata on the gastrointestinal tract in rats and mice.

    Science.gov (United States)

    Ortega, C A; María, A O M; Giordano, O S; Gianello, J C

    2003-04-01

    A pharmacological evaluation of Zuccagnia punctata Cav. (Fabaceae) on the gastrointestinal tract was made in rats and mice. 2',4'-dihydroxychalcone and 2',4'-dihydroxy-3'-methoxychalcone were isolated from Zuccagnia punctata. The data reported in the present work indicate that the acetone extract and infusion of Zuccagnia punctata reduced intestinal transit in rats and mice and offered protection against ethanol-induced ulceration in rats. The Z. punctata effect could be due, in part, to the presence of 2',4'-dihydroxychalcone and 2',4'-dihydroxy-3'-methoxychalcone in this plant. PMID:12722150

  3. Notes of the types of Aeshna punctata Martin, 1908 (Odonata, Aeshnidae

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    Angelo B. M. Machado

    1984-01-01

    Full Text Available The type series of Aeshna punctata Martin, 1908 was examined and some inconsistent points in the original description were elucidated. A lectolype was designated, redescribed and illustrated. The two females originally referred to this species actually belong to the Mexican species Aeshna jalapensis Williamson, 1908. Thus, A. punctata should be dropped from the faunistic list of Mexico.

  4. Leucodermia punctata após tratamento com Puvasol tópico Leukoderma punctata following topical PUVAsol treatment

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    Nurimar Conceição Fernandes

    2010-08-01

    Full Text Available Duas adolescentes e uma menina com vitiligo clinicamente diagnosticado foram tratadas com 8-metoxipsoraleno a 0,2% em creme Lanette com subsequente exposição solar. Um ano após, apresentaram máculas acrômicas na área do vitiligo. A biópsia de pele em um dos casos revelou melanócitos com escassa pigmentação melânica. Os achados clínicos e histológicos sugerem o diagnóstico de leucodermia punctata.Two adolescent females and a girl, all with clinically diagnosed vitiligo, were treated with 0.2% 8-methoxypsoralen cream followed by exposure to solar ultraviolet light. One year later, they developed hypopigmented and achromic spots on the areas affected by the vitiligo. Biopsy of skin tissue taken from one of these cases showed a marked reduction in melanin. Clinical and histological findings point to a diagnosis of leukoderma punctata.

  5. Leucodermia punctata após tratamento com Puvasol tópico Leukoderma punctata following topical PUVAsol treatment

    OpenAIRE

    Nurimar Conceição Fernandes; Juliana Carnevale Pina

    2010-01-01

    Duas adolescentes e uma menina com vitiligo clinicamente diagnosticado foram tratadas com 8-metoxipsoraleno a 0,2% em creme Lanette com subsequente exposição solar. Um ano após, apresentaram máculas acrômicas na área do vitiligo. A biópsia de pele em um dos casos revelou melanócitos com escassa pigmentação melânica. Os achados clínicos e histológicos sugerem o diagnóstico de leucodermia punctata.Two adolescent females and a girl, all with clinically diagnosed vitiligo, were treated with 0.2% ...

  6. Cytoprotective effects of chalcones from Zuccagnia punctata and melatonin on the gastroduodenal tract in rats.

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    de la Rocha, N; María, A O M; Gianello, J C; Pelzer, L

    2003-07-01

    The effects of 2',4'-dihydroxychalcone and 2',4'-dihydroxy-3'-methoxychalcone from Zuccagnia punctata Cav. (Fabaceae) and melatonin administration on ethanol-induced gastroduodenal injury were investigated in rats. Both chalcones showed significant preventive effects in treatment with melatonin previous to the necrotising agent. These effects could be due, in part, to the radical scavenging activity of the melatonin. PMID:12770521

  7. Evaluation of genotoxic and antigenotoxic effects of hydroalcoholic extracts of Zuccagnia punctata Cav.

    Science.gov (United States)

    Zampini, Iris Catiana; Villarini, Milena; Moretti, Massimo; Dominici, Luca; Isla, María Inés

    2008-01-17

    Zuccagnia punctata Cav. (Fabaceae), a widely used plant species in Argentine folk medicine, has been shown to have a broad spectrum of antibacterial, antifungal, antioxidant and cytoprotective activities. In this study, the hydroalcoholic extract of Zuccagnia punctata and 2',4'-dihydroxychalcone isolated from it were investigated for genotoxicity/antigenotoxicity in the in vitro comet assay test on human hepatoma HepG2 cells. No acute toxicity of the extract could be determined. HepG2 cells were treated with three different concentrations (2.5, 5.0 and 10.0 microg/mL) or 2',4'-dihydroxychalcone (0.01, 0.10 and 1.00 microg/mL). To explore the potential mechanisms of action, two approaches were followed: co-treatment with 4-nitroquinoline-N-oxyde (4-NQO), a direct genotoxic compound, and a pre-treatment protocol with benzo[a]pyrene (B[a]P), an indirect genotoxic compound. The natural products neither affected cell viability nor induced DNA damage in the concentration range tested. Zuccagnia punctata tinctures were able to diminish the DNA damage induced in HepG2 cells by 4-NQO and B[a]P in 31% and 10%, respectively at 10 microg/mL. Pre-treatment of HepG2 cells with 2',4'-dihydroxychalcone was highly effective in decreasing B[a]P-induced DNA damage at a statistically significant level, with an almost clear dose-response relationship. The inhibition values were 28.2-43.9% for the tested concentrations of 0.01-1 microg/mL, respectively. The results clearly indicate that the phytoextract from Zuccagnia punctata, under the experimental conditions tested, is not genotoxic and that 2',4'-dihydroxychalcone contributes to a high degree to the antigenotoxic effects of Zuccagnia punctata tincture. PMID:18023546

  8. Functional neuroanatomy of the rhinophore of Aplysia punctata

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    Rössler Wolfgang

    2006-04-01

    Full Text Available Abstract Background For marine snails, olfaction represents a crucial sensory modality for long-distance reception, as auditory and visual information is limited. The posterior tentacle of Aplysia, the rhinophore, is a chemosensory organ and several behavioural studies showed that the rhinophores can detect pheromones, initiate orientation and locomotion toward food. However the functional neuroanatomy of the rhinophore is not yet clear. Here we apply serotonin-immunohistochemistry and fluorescent markers in combination with confocal microscopy as well as optical recording techniques to elucidate the structure and function of the rhinophore of the sea slug Aplysia punctata. Results With anatomical techniques an overview of the neuroanatomical organization of the rhinophore is presented. Labelling with propidium iodide revealed one layer of cell nuclei in the sensory epithelium and densely packed cell nuclei beneath the groove of the rhinophore, which extends to about two third of the total length of the rhinophore. Serotonin immunoreactivity was found within the olfactory glomeruli underneath the epithelium as well as in the rhinophore ganglion. Retrograde tracing from the rhinophore ganglion with 4-(4-(dihexadecylaminostyryl-N-methylpyridinium iodide (DiA demonstrated the connection of glomeruli with the ganglion. Around 36 glomeruli (mean diameter 49 μm were counted in a single rhinophore. Fluorimetric measurements of intracellular Ca2+ levels using Fura-2 AM loading revealed Ca2+-responses within the rhinophore ganglion to stimulation with amino acids. Bath application of different amino acids revealed differential responses at different positions within the rhinophore ganglion. Conclusion Our neuroanatomical study revealed the number and position of glomeruli in the rhinophore and the rhinophore ganglion as processing stage of sensory information. Serotonin-immunoreactive processes were found extensively within the rhinophore, but was not

  9. Histo-morphology of the alimentary canal in two freshwater snakehead fish Channa punctata and Channa striata

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    Mita Borman; Ismot Ara; Mohammad Kamrujjaman; Md Rafiqun Nabi

    2015-01-01

    The histo-morphological study of the alimentary canal of two carnivore freshwater snakehead fish Channa punctata and C. striata was carried out from October 2013 to July 2014. It revealed that three major parts like oesophagus, stomach and intestine composed of short thick-walled body. The oesophagus begins with buccopharynx. Structure and arrangement of both villiform and canine teeth on jaws in C. striata are more extendable and stronger than C. punctata and thereby made the former one more...

  10. Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) II: transcriptome alterations of pathways involved in carbohydrate metabolism and endogenous hormone crosstalk

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    Liu, Yang; Fang, Yang; Huang, Mengjun; Jin, Yanling; Sun, Jiaolong; TAO, XIANG; Zhang, Guohua; He, Kaize; Zhao, Yun; Zhao, Hai

    2015-01-01

    Background Landoltia punctata is a widely distributed duckweed species with great potential to accumulate enormous amounts of starch for bioethanol production. We found that L. punctata can accumulate starch rapidly accompanied by alterations in endogenous hormone levels after uniconazole application, but the relationship between endogenous hormones and starch accumulation is still unclear. Results After spraying fronds with 800 mg/L uniconazole, L. punctata can accumulate starch quickly, wit...

  11. Severe but reversible acute kidney injury resulting from Amanita punctata poisoning

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    Kang, Eunjung; Cheong, Ka-Young; Lee, Min-Jeong; Kim, Seirhan; Shin, Gyu-Tae; Kim, Heungsoo; Park, In-Whee

    2015-01-01

    Mushroom-related poisoning can cause acute kidney injury. Here we report a case of acute kidney injury after ingestion of Amanita punctata, which is considered an edible mushroom. Gastrointestinal symptoms occurred within 24 hours from the mushroom intake and were followed by an asymptomatic period, acute kidney injury, and elevation of liver and pancreatic enzymes. Kidney function recovered with supportive care. Nephrotoxic mushroom poisoning should be considered as a cause of acute kidney i...

  12. CHARACTERISTICS OF GAMBUSIA PUNCTATA (POECILIDAE) FOR ITS SELECTION AS BIOMONITOR IN AQUATIC TOXICOLOGY IN CUBA

    OpenAIRE

    Argota, George; Iannacone, José; Fimia, Rigoberto

    2013-01-01

    The use of natural organisms in their capacity as biomonitors allows the evaluation of the environmental conditions of aquatic ecosystems. The aim of this study was to describe the characteristics of Gambusia punctata Poey, 1854 (Poeciliidae) for selection as a biomonitor in aquatic ecotoxicology. The species was selected to be representative and monitored from 2004 to 2012 on a quarterly basis, with two of them corresponding to periods of rain and little rain outside the Almen...

  13. Antifungal chalcones and new caffeic acid esters from Zuccagnia punctata acting against soybean infecting fungi.

    Science.gov (United States)

    Svetaz, Laura; Tapia, Alejandro; López, Silvia N; Furlán, Ricardo L E; Petenatti, Elisa; Pioli, Rosanna; Schmeda-Hirschmann, Guillermo; Zacchino, Susana A

    2004-06-01

    The crude methanolic extract of Zuccagnia punctata was active toward the fungal pathogens of soybean Phomopsis longicolla and Colletotrichum truncatum. Assay guided fractionation led to the isolation of two chalcones, one flavanone and a new caffeoyl ester derivative as the compounds responsible for the antifungal activity. Another new caffeoyl ester derivative was isolated from the antifungal chloroform extract but proved to be inactive against the soybean infecting fungi up to 50 microg/mL PMID:15161186

  14. Biomineralization in the Sea Hare Aplysia punctata Initiated by Nano-Dolomite

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    Tonejc, Anđelka; Medaković, Davorin; Popović, Stanko; JAKLIN Andrej; Bijelić, Mirjana; Lončarek, Ivana

    2014-01-01

    X-ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED), high resolution transmission electron microscopy (HRTEM), environmental scanning electron microscopy (ESEM) and energy dispersive X-ray analysis (EDS) were used in study of starting biomineralization processes in embryos of the sea hare species Aplysia punctata. 10 days old embryos appeared amorphous according to XRD patterns. TEM of the same sample showed that first grains of nanocryst...

  15. Effect of structurally related flavonoids from Zuccagnia punctata Cav. on Caenorhabditis elegans.

    Science.gov (United States)

    D'Almeida, Romina E; Alberto, María R; Morgan, Phillip; Sedensky, Margaret; Isla, María I

    2014-03-01

    Zuccagnia punctata Cav. (Fabaceae), commonly called jarilla macho or pus-pus, is being used in traditional medicine as an antiseptic, anti-inflammatory and to relieve muscle and bone pain. The aim of this work was to study the anthelmintic effects of three structurally related flavonoids present in aerial parts of Z. punctata Cav. The biological activity of the flavonoids 7-hydroxyflavanone (HF), 3,7-dihydroxyflavone (DHF) and 2´,4´-dihydroxychalcone (DHC) was examined in the free-living nematode Caenorhabditis elegans. Our results showed that among the assayed flavonoids, only DHC showed an anthelmintic effect and alteration of egg hatching and larval development processes in C. elegans. DHC was able to kill 50% of adult nematodes at a concentration of 17 μg/mL. The effect on larval development was observed after 48 h in the presence of 25 and 50 μg/mL DHC, where 33.4 and 73.4% of nematodes remained in the L3 stage or younger. New therapeutic drugs with good efficacy against drug-resistant nematodes are urgently needed. Therefore, DHC, a natural compound present in Z. punctata, is proposed as a potential anthelmintic drug. PMID:26204036

  16. Effects of nutrient media on vegetative growth of Lemna minor and Landoltia punctata during in vitro and ex vitro cultivation

    Directory of Open Access Journals (Sweden)

    Chokchai Kittiwongwattana

    2013-01-01

    Full Text Available Lemnaceous plants, namely Lemna minor and Landoltia punctata, have been used in various types of biological research. The effects of Murashige and Skoog (MS and Hoagland media on vegetative growth rate of both species during in vitro and ex vitro cultivation were investigated. Under axenic conditions, frond proliferation of L. minor and Lan. punctata in Hoagland medium are 8 and 11.5% respectively faster than that in MS medium. Biomass production in Hoagland medium also increases 2.2-fold (L. minor and 1.4-fold (Lan. punctata compared to MS medium. The roots of both species in MS medium are distinctly shorter than those in Hoagland medium. In contrast, ex vitro regeneration of frond colonies in MS medium is 22.2% (for L. minor and 17.1% (for Lan. punctata faster than in Hoagland medium. Similarly, ex vitro biomass production of both species in MS increases 1.8-fold (for L. minor and 1.3-fold (for Lan. punctata compared to that in Hoagland medium. Root elongation of the frond colonies in MS and Hoagland media is comparable. The distinct effects of MS and Hoagland media on vegetative growth of both species and the pre-determination of ex vitro growth rates in each medium are demonstrated.

  17. Some psychosocial aspects of nonlethal chondrodysplasias: I. Assessment using a Life-Styles Questionnaire.

    Science.gov (United States)

    Hunter, A G

    1998-06-16

    Studies concerning the psychosocial aspects of skeletal dysplasias that cause disproportionate short stature have been few and have usually involved small numbers of patients. As part of a study involving patients with chondrodysplasias and their families, an assessment battery of standardised instruments designed to measure depression, anxiety, self-esteem, personal support networks, marital adjustment, and family structure were completed by patients and, in many cases, their sibs, spouses, and/or parents. This first in a series of six papers reports the results of a Life-Styles Questionnaire which provides some insights into the levels of satisfaction with various aspects of life, including friendships, employment, the use of some substances and services, and the impact of the skeletal dysplasia on career, marriage, and childbearing. Results are presented for patients and the unaffected parents of patients. Overall, the study has shown a high level of satisfaction with many aspects of life. However, there are important differences in attitudes between the married and unmarried patients, and in some cases the unaffected parents, in a number of areas including health, overall satisfaction with life, and concerns surrounding child bearing and employment. PMID:9637414

  18. Structural and segregation analysis of the type II collagen gene (COL2A1) in some heritable chondrodysplasias.

    OpenAIRE

    Wordsworth, P; Ogilvie, D.; Priestley, L; Smith, R.; Wynne-Davies, R; Sykes, B

    1988-01-01

    Seventy-seven persons with a variety of heritable chondrodysplasias were screened for gross rearrangements of the structural gene encoding the major cartilage collagen, collagen II. None was found. Segregation of the locus (COL2A1) was studied in 19 pedigrees using three restriction site dimorphisms (shown by PvuII, HindIII, and BamHI) and a length polymorphism as linkage markers. Discordant segregation between COL2A1 and the mutant locus was seen in pedigrees with multiple epiphyseal dysplas...

  19. Subcellular distribution of uranium in the roots of Spirodela punctata and surface interactions

    Energy Technology Data Exchange (ETDEWEB)

    Nie, Xiaoqin, E-mail: xiaoqin_nie@163.com [Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Mianyang 621010 (China); Key Laboratory of Radiation Physics and Technology (Sichuan University), Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064 (China); Dong, Faqin, E-mail: fqdong2004@163.com [Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Mianyang 621010 (China); Liu, Ning [Key Laboratory of Radiation Physics and Technology (Sichuan University), Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064 (China); Liu, Mingxue [Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Mianyang 621010 (China); Zhang, Dong; Kang, Wu [Institute of Nuclear Physics and Chemistry,China Academy of Engineering Physics, Mianyang 621900 (China); Sun, Shiyong; Zhang, Wei; Yang, Jie [Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Mianyang 621010 (China)

    2015-08-30

    Graphical abstract: - Highlights: • The proportion of uranium concentration approximate as 8:2:1 in the cell wall organelle and cytosol fractions of roots of S. punctata. • The particles including 35% Fe (wt%) released from the cells after 100 mg/L U treatment 48 h. • Most of the uranium bound onto the root surface and contacted with phosphorus ligands and formed as nano-scales U-P lamellar crystal. • FTIR and XPS analyses result indicates the uranium changed the band position and shapes of phosphate group, and the region of characteristic peak belongs to U(VI) and U(IV) were also observed. - Abstract: The subcellular distribution of uranium in roots of Spirodela punctata (duckweed) and the process of surface interaction were studied upon exposure to U (0, 5–200 mg/L) at pH 5. The concentration of uranium in each subcelluar fraction increased significantly with increasing solution U level, after 200 mg/L uranium solution treatment 120 h, the proportion of uranium concentration approximate as 8:2:1 in the cell wall organelle and cytosol fractions of roots of S. punctata. OM SEM and EDS showed after 5–200 mg/L U treatment 4–24 h, some intracellular fluid released from the root cells, after 100 mg/L U treatment 48 h, the particles including 35% Fe (wt%) and other organic matters such as EPS released from the cells, most of the uranium bound onto the root surface and contacted with phosphorus ligands and formed as nano-scales U-P lamellar crystal, similar crystal has been found in the cell wall and organelle fractions after 50 mg/L U treatment 120 h. FTIR and XPS analyses result indicates the uranium changed the band position and shapes of phosphate group, and the region of characteristic peak belongs to U(VI) and U(IV) were also observed.

  20. SSCP and segregation analysis of the human type X collagen gene (COL10A1) in heritable forms of chondrodysplasia

    Energy Technology Data Exchange (ETDEWEB)

    Sweetman, W.A.; Rash, B.; Thomas, J.T.; Boot-Handford, R.; Grant, M.E.; Wallis, G.A. (Univ. of Manchester (United Kingdom)); Sykes, B. (Univ. of Oxford (United Kingdom)); Beighton, P. (Univ. of Cape Town (South Africa)); Hecht, J.T. (Univ. of Texas, Houston, TX (United States)); Zabell, B. (Johannes Gutenburg Universitaet, Mainz (Germany))

    1992-10-01

    Type X collagen is a homotrimeric, short chain, nonfibrillar collagen that is expressed exclusively by hypertrophic chondrocytes at the sites of endochondral ossification. The distribution and pattern of expression of the type X collagen gene (COL10A1) suggests that mutations altering the structure and synthesis of the protein may be responsible for causing heritable forms of chondrodysplasia. The authors investigated whether mutations within the human COL10A1 gene were responsible for causing the disorders achondroplasia, hypochondroplasia, pseudoachondroplasia, and thanatophoric dysplasia, by analyzing the coding regions of the gene by using PCR and the single-stranded conformational polymorphism technique. By this approach, seven sequence changes were identified within and flanking the coding regions of the gene of the affected persons. The authors demonstrated that six of these sequence changes were not responsible for causing these forms of chondrodysplasia but were polymorphic in nature. The sequence changes were used to demonstrate discordant segregation between the COL10A1 locus and achondroplasia and pseudoachondroplasia, in nuclear families. This lack of segregation suggests that mutations within or near the COL101A1 locus are not responsible for these disorders. The seventh sequence change resulted in a valine-to-methionine substitution in the carboxyl-terminal domain of the molecule and was identified in only two hypochondroplasic individuals from a single family. Segregation analysis in this family was inconclusive, and the significance of this substitution remains uncertain. 47 refs., 3 figs., 2 tabs.

  1. The effect of Zuccagnia punctata, an Argentine medicinal plant, on virulence factors from candida species.

    Science.gov (United States)

    Gabriela, Nuño; Rosa, Alberto María; Catiana, Zampini Iris; Soledad, Cuello; Mabel, Ordoñez Roxana; Esteban, Sayago Jorge; Veronica, Baroni; Daniel, Wunderlin; Ines, Isla María

    2014-07-01

    Zuccagnia punctata Cav. has been used as a traditional medicine in Argentina for the treatment of bacterial and fungal infections. In this study, we evaluated the ability of Z. punctata extract (ZpE) and compounds isolated from it to inhibit the growth and virulence factors of Candida species. ZpE showed inhibitory activity against planktonic cells of all assayed Candida species with MIC values of 400 microg/mL and with MFC values between 400 and 1,200 microg/mL. The principal identified compounds by HPLC-MS/MS and UV-VIS were chalcones (2',4'-dihydroxy-3'-methoxychalcone, 2',4'- dihydroxychalcone), flavones (galangin, 3,7-dihydroxyflavone and chrysin) and flavanones (naringenin, 7-hydroxyflavanone and pinocembrine). These compounds were more effective as inhibitors than the extracts upon biofilm formation as well as on preformed Candida biofilm and yeast germ tube formation. Furthermore, ZpE and chalcones are able to inhibit exoenzymes, which are responsible for the invasion mechanisms of the pathogens. All these effects could moderate colonization, thereby suppressing the pathogen invasive potential. Our results indicate that ZpE and chalcones could be used in antifungal therapy. PMID:25230496

  2. Argentinean propolis from Zuccagnia punctata Cav. (Caesalpinieae) exudates: phytochemical characterization and antifungal activity.

    Science.gov (United States)

    Agüero, María Belén; Gonzalez, Mariela; Lima, Beatriz; Svetaz, Laura; Sánchez, Marianela; Zacchino, Susana; Feresin, Gabriela Egly; Schmeda-Hirschmann, Guillermo; Palermo, Jorge; Wunderlin, Daniel; Tapia, Alejandro

    2010-01-13

    This paper reports the in vitro antifungal activity of propolis extracts from the province of Tucuman (Argentina) as well as the identification of their main antifungal compounds and botanical origin. The antifungal activity was determined by the microdilution technique, using reference microorganisms and clinical isolates. All dermatophytes and yeasts tested were strongly inhibited by different propolis extracts (MICs between 16 and 125 microg mL(-1)). The most susceptible species were Microsporum gypseum, Trichophyton mentagrophytes, and Trichophyton rubrum. The main bioactive compounds were 2',4'-dihydroxy-3'-methoxychalcone 2 and 2',4'-dihydroxychalcone 3. Both displayed strong activity against clinical isolates of T. rubrum and T. mentagrophytes (MICs and MFCs between 1.9 and 2.9 microg mL(-1)). Additionally, galangin 5, pinocembrin 6, and 7-hydroxy-8-methoxyflavanone 9 were isolated from propolis samples and Zuccagnia punctata exudates, showing moderate antifungal activity. This is the first study matching the chemical profile of Z. punctata Cav. exudates with their corresponding propolis, giving strong evidence on the botanical origin of the studied propolis. PMID:19916546

  3. Reappearance and seasonality of Phyllorhiza punctata von Lendenfeld (Cnidaria, Scyphozoa, Rhizostomeae) medusae in southern Brazil Reaparecimento e sazonalidade da medusa Phyllorhiza punctata von Lendenfeld (Cnidaria, Scyphozoa, Rhizostomeae) no sul do Brasil

    OpenAIRE

    Maria A. Haddad; Miodeli Nogueira Júnior

    2006-01-01

    The scyphozoan Phyllorhiza punctata von Lendenfeld, 1884 (Mastigiidae), known only from Indo-Pacific waters prior to the 1950s, is today found far from its original distribution, probably due to human activities. First seen in 1955 in Brazilian waters, medusae were found in southern and southeastern coasts, disappearing (at least as a medusa) in the early 1960's. Another population was found in the late 1990's, in the state of Bahia, and again in late 2001, many sightings were reported along ...

  4. Singlet oxygen quenching and radical scavenging capacities of structurally-related flavonoids present in Zuccagnia punctata Cav.

    Science.gov (United States)

    Vieyra, Faustino E Morán; Boggetti, Héctor J; Zampini, Iris C; Ordoñez, Roxana M; Isla, María I; Alvarez, Rosa M S; De Rosso, Veridiana; Mercadante, Adriana Z; Borsarelli, Claudio D

    2009-06-01

    The singlet oxygen (1O2) quenching and free radical (DPPH(*), ABTS(* +) and O2(* -)) scavenging ability of three structurally-related flavonoids (7-hydroxyflavanone HF, 2',4'-dihydroxychalcone DHC and 3,7-dihydroxyflavone DHF) present in the Argentinean native shrub Zuccagnia punctata Cav. were studied in solution by combining electrochemical and kinetic measurements, mass spectroscopy, end-point antioxidant assays and computational calculations. The results showed that the antioxidant properties of these flavonoids depend on several factors, such as their electron- and hydrogen atom donor capacity, the ionization degree of the more acidic group, solvatation effects and electrostatic interactions with the oxidant species. The theoretical calculations for both the gas and solution phases at the B3LYP level of theory for the Osanger reaction field model agreed with the experimental findings, thus supporting the characterization of the antioxidant mechanism of the Z. punctata flavonoids. PMID:19431060

  5. Endoplasmic Reticulum Stress-Unfolding Protein Response-Apoptosis Cascade Causes Chondrodysplasia in a col2a1 p.Gly1170Ser Mutated Mouse Model

    OpenAIRE

    Liang, Guoyan; Lian, Chengjie; Huang, Di; Gao, Wenjie; Liang, Anjing; Peng, Yan; Ye, Wei; Wu, Zizhao; Su, Peiqiang; Huang, Dongsheng

    2014-01-01

    The collagen type II alpha 1 (COL2A1) mutation causes severe skeletal malformations, but the pathogenic mechanisms of how this occurs are unclear. To understand how this may happen, a col2a1 p.Gly1170Ser mutated mouse model was constructed and in homozygotes, the chondrodysplasia phenotype was observed. Misfolded procollagen was largely synthesized and retained in dilated endoplasmic reticulum and the endoplasmic reticulum stress (ERS)-unfolded protein response (UPR)-apoptosis cascade was act...

  6. Histo-morphology of the alimentary canal in two freshwater snakehead fish Channa punctata and Channa striata

    Directory of Open Access Journals (Sweden)

    Mita Borman

    2015-12-01

    Full Text Available The histo-morphological study of the alimentary canal of two carnivore freshwater snakehead fish Channa punctata and C. striata was carried out from October 2013 to July 2014. It revealed that three major parts like oesophagus, stomach and intestine composed of short thick-walled body. The oesophagus begins with buccopharynx. Structure and arrangement of both villiform and canine teeth on jaws in C. striata are more extendable and stronger than C. punctata and thereby made the former one more successful predator. The availability and arrangement pattern of mucous pits and taste bud pores in oesophagus are also prominent in C. striata. The TS of stomach of both the species has broad GM devoid of goblet mucous cells, but surface layer CC and basal layer GG open through gastric pits. The length of intestine (16.0 cm and intestinal pyloric caeca (5.5 cm in C. striata are larger than C. punctata (7.0 cm and 1.5 cm, respectively. However, the TS of intestinal Sr. 0.05 mm; MM. 0.8 mm; Mu 0.5 mm suggest in favour of carnivore habit of both the species.

  7. Morphological Variation of Sordellina punctata (Peters, 1880 with Notes on the Phylogenetic Position of the Genus within the Family Dipsadidae (Serpentes

    Directory of Open Access Journals (Sweden)

    Bruno Henrique V. Miranda

    2012-01-01

    Full Text Available Sordellina punctata (Peters, 1880 is a poorly known species from Brazil that, until recently, had an uncertain phylogenetic position. Sixty-one specimens of Sordellina punctata were examined, representing different localities encompassing the known geographic distribution of this taxon. Eight skulls and five hemipenes were prepared and described in detail and data on scale variation and color pattern as well as morphometric data were analyzed. The skull bones described, as well as the hemipenis of S. punctata, were compared to available data in the literature referred to members of the tribe Echinanterini and related taxa. Sordellina punctata presents an average snout-vent length of 31.5 cm for males and 40.2 for females and has an average of 149 ventral scales in males and 157 in females. The data presented here reinforce the necessity of a greater amount of osteological descriptive studies, especially of members of the tribe Echinanterini, in order to evaluate the recently proposed hypothesis of relationship of S. punctata and these taxa.

  8. CHARACTERISTICS OF GAMBUSIA PUNCTATA (POECILIDAE FOR ITS SELECTION AS BIOMONITOR IN AQUATIC TOXICOLOGY IN CUBA

    Directory of Open Access Journals (Sweden)

    Argota, George

    2013-07-01

    Full Text Available The use of natural organisms in their capacity as biomonitors allows the evaluation of the environmental conditions of aquatic ecosystems. The aim of this study was to describe the characteristics of Gambusia punctata Poey, 1854 (Poeciliidae for selection as a biomonitor in aquatic ecotoxicology. The species was selected to be representative and monitored from 2004 to 2012 on a quarterly basis, with two of them corresponding to periods of rain and little rain outside the Almendares -Vento Basin in San Juan Havana and Santiago de Cuba, Cuba, respectively. The description of the characteristics of the referred species considered whether they met seven criteria for biomonitoring: 1 cosmopolitan distribution, 2 easy taxonomic identification, 3 appropriate size, 4 limited mobility and relatively long life cycle, 5 tolerant to contamination, 6 easy handling in laboratory studies, and 7 correlation between the contaminant of interest and other environmental matrices. The characteristics were met for each of the seven criteria, and thus have conditional use as biomonitors in ecotoxicology studies and for the ecosystem at San Juan Almendares, Cuba.

  9. Antifungal activity of Zuccagnia punctata Cav.: evidence for the mechanism of action.

    Science.gov (United States)

    Svetaz, Laura; Agüero, María Belén; Alvarez, Sandra; Luna, Lorena; Feresin, Gabriela; Derita, Marcos; Tapia, Alejandro; Zacchino, Susana

    2007-08-01

    Petroleum ether and dichloromethane extracts of fruits, aerial parts and exudate of Zuccagnia punctata Cav. (Fabaceae) showed moderate antifungal activities against the yeasts C. albicans, S. cerevisiae and C. neoformans (MICs: 62.5 - 250 microg/mL) and very strong antifungal activities against the dermatophytes M. gypseum, T. rubrum and T. mentagrophytes (MICs: 8 - 16 microg/mL) thus supporting the ethnopharmacological use of this plant. Antifungal activity-directed fractionation of active extracts by using bioautography led to the isolation of 2',4'-dihydroxy-3'-methoxychalcone (1) and 2',4'-dihydroxychalcone (2) as the compounds responsible for the antifungal activity. Second-order studies included MIC (80), MIC (50) and MFC of both chalcones in an extended panel of clinical isolates of the most sensitive fungi, and also comprised a series of targeted assays. They showed that the most active chalcone 2 is fungicidal rather than fungistatic, does not disrupt the fungal membranes up to 4 x MFC and does not act by inhibiting the fungal cell wall. So, 2',4'-dihydroxychalcone would act by a different mechanism of action than the antifungal drugs in current clinical use, such as amphotericin B, azoles or echinocandins, and thus appears to be very promising as a novel antifungal agent. PMID:17628836

  10. Unilateral punctate porokeratosis - Case report Poroceratose punctata unilateral - Relato de caso

    Directory of Open Access Journals (Sweden)

    Vera Barreto Teixeira

    2013-06-01

    Full Text Available This case report involves a 20-year-old man with unilateral punctate porokeratosis. The patient presented an 8-year history of numerous asymptomatic keratotic papules and pits with linear distribution on his left pal-mar surface and fifth finger of the left hand. Histopathological examination of the keratotic plug revealed findings of distinct epidermal depressions containing cornoid lamellae. This report review draws attention to differential diagnoses of punctate porokeratosis.Relata-se o caso de um homem de 20 anos de idade com poroceratose punctata, caracterizada por múltiplas pápulas queratósicas e depressões, com disposição linear localizada à região palmar da mão e 5º dedo esquerdos, com cerca de 8 anos de evolução. O estudo histológico mostrou presença de depressão da epiderme, preenchida por característica lamela cornóide. Foi realizada revisão da literatura e ressaltado o seu diagnóstico diferencial.

  11. To duckweeds (Landoltia punctata), nanoparticulate copper oxide is more inhibitory than the soluble copper in the bulk solution

    OpenAIRE

    Shi, Jiyan; Abid, Aamir D; Kennedy, Ian M.; Hristova, Krassimira R.; Silk, Wendy K.

    2011-01-01

    CuO nanoparticles (CuO-NP) were synthesized in a hydrogen diffusion flame. Particle size and morphology were characterized using scanning mobility particle sizing, Brunauer–Emmett–Teller analysis, dynamic light scattering, and transmission electron microscopy. The solubility of CuO-NP varied with both pH and presence of other ions. CuO-NP and comparable doses of soluble Cu were applied to duckweeds, Landoltia punctata. Growth was inhibited 50% by either 0.6 mg L−1 soluble copper or by 1.0 mg ...

  12. Anti-Oxidant activity of 80% methanol extracts from Clerodendron myricoides, Satureja punctata, Urtica dioica, Ajuga remota and Gnidia stenophylla

    Directory of Open Access Journals (Sweden)

    Ibrahim Nasser

    2010-01-01

    Full Text Available El estrés oxidativo está involucrado en el desarrollo de muchas enfermedades, como: aterosclerosis, hipertensión, asma, diabetes mellitus, y muchas más. La necesidad de nuevos antioxidantes o mezclas de nuevos antioxidantes tiene una gran importancia en el manejo de enfermedades crónicas. Los extractos crudos de Clerodendron myricoides, Satureja punctata, Urtica sciemensis, Ajuga remota and Gnidia stenophylla en metanol (80% fueron estudiadas para evaluar sus propiedades antioxidantes usando el radical libre 1,1-diphenyl-2-picrylhydrazyl (DPPH y ácido ascórbico como estándares. La actividad secuestradora del radical fue expresada como la cantidad de extractos activos necesarios para disminuir la absorbancia inicial DPPH (50%. Todos los extractos de las plantas mostraron una actividad antioxidante significativa. C. myricoides fue la más potente, seguida en orden descendente por S. punctata, U. dioica, A. remota and G. stenophylla. La concentración iniciall 50 (CI50 de los extractos estuvo en el rango entre 6.5 - 9.1 ¿g/mL y para el ácido ascórbico fue 8.6 ¿g/mL. Los ensayos fitoquímicos de los extractos de las plantas investigadas indicaron la presencia de polifenoles, alcaloides, taninos y flavonoides. Se ha reprtado que estos compuestos poseen una potente actividad antioxidante.

  13. Lethal chondrodysplasia in a family of Holstein cattle is associated with a de novo splice site variant of COL2A1

    DEFF Research Database (Denmark)

    Agerholm, Jørgen Steen; Menzi, Fiona; McEvoy, Fintan;

    2016-01-01

    variant as candidate causal mutation of a severe lethal chondrodysplasia phenotype. Germline mosaicism is a relatively frequent mechanism in the origin of genetic disorders and explains the prevalence of a certain fraction of affected offspring. Paternal dominant de novo mutations are a risk in cattle...

  14. Chromosomal aberrations in a fish, Channa punctata after in vivo exposure to three heavy metals.

    Science.gov (United States)

    Yadav, Kamlesh K; Trivedi, Sunil P

    2009-08-01

    The studies were designed to assess the extent of chromosomal aberrations (CA) under the exposure of three common heavy metalic compounds, viz. mercuric chloride, arsenic trioxide and copper sulphate pentahydrate, in vivo using fish, Channa punctata (2n=32), as a test model. Prior acclimatized fishes were divided into five groups. Group I and II served as negative and positive control, respectively. An intramuscular injection of Mitomycin-C (@ 1mg/kg body wt.) was administered to group II only. Fishes of groups III, IV and V were subjected to sublethal concentrations (10% of 96h LC(50)), of HgCl(2) (0.081mg/L), As(2)O(3) (6.936mg/L) and CuSO(4)x5H(2)O (0.407mg/L). Fishes of all the groups were exposed uninterrupted for 24, 48, 72, 96 and 168h. Observations of kidney cells of exposed fishes revealed chromatid and chromosome breaks, chromatid and chromosome gaps along with ring and di-centric chromosomes. A significant increase over negative control in the frequency of chromosomal aberrations (CA) was observed in fish exposed to Mitomycin-C, Hg(II), As(III) and Cu(II). As the average + or - SE total number of CA, average number of CA per metaphase and %incidence of aberrant cells in Hg(II) was 104.40 + or - 8.189, 0.347 + or - 0.027 and 10.220 + or - 0.842, respectively; in As(III) 109.20 + or - 8.309, 0.363 + or - 0.027 and 10.820 + or - 2.347, respectively and in Cu(II) 89.00 + or - 19.066, 0.297 + or - 0.028 and 8.900 + or - 0.853, respectively. Hence, it reveals that the order of induction of frequency of CA was Cu

  15. When and where to have sex? Different modes of reproduction and life history traits in the facultative parthenogenetic ant Platythyrea punctata

    OpenAIRE

    Kellner, Katrin

    2010-01-01

    Hymenopterans generally exhibit a haplo-diploid sex determination mechanism, in which females are produced by sexual reproduction and are diploid and males are produced parthenogenetically and are haploid. In a small number of ant species, workers are able to produce diploid female offspring parthenogenetically from unfertilized eggs, which is termed thelytoky. One of these species, the ponerine ant Platythyrea punctata, is of peculiar interest, because it has been found to have both thelytok...

  16. A multiple-parameter approach to analyzing the mid-punctata zone anomalous signatures in pure limestones (Moravian Karst, Brunovistulian terrane, central Europe)

    Czech Academy of Sciences Publication Activity Database

    Hladil, Jindřich; Koptíková, Leona; Geršl, Milan; Langrová, Anna; Pruner, Petr; Galle, Arnošt; Bábek, O.; Frána, Jaroslav; Otava, J.; Chadima, Martin

    New York : SUNY-Geneseo ; San Diego State University, 2007 - (Over, D.; Morrow, J.), s. 40-42 [Subcommission on Devonian Stratigraphy and IGCP 499 Devonian Land Sea Interaction. Eureka (US), 09.09.2007-17.09.2007] R&D Projects: GA AV ČR IAAX00130702 Institutional research plan: CEZ:AV0Z30130516; CEZ:AV0Z10480505 Keywords : mid-punctata Event * geophysics * geochemistry Subject RIV: DB - Geology ; Mineralogy

  17. Myxidium mackiei (Myxosporea) in Indo-Gangetic flap-shelled turtles Lissemys punctata andersonii: parasite-host interaction and ultrastructure.

    Science.gov (United States)

    Helke, K L; Poynton, S L

    2005-02-28

    Myxosporeans are common parasites of fish, and uncommon parasites of amphibians, reptiles and invertebrates, that can cause significant morbidity and mortality. The common genus Myxidium infects the excretory system of turtles, yet knowledge of its pathogenicity in these hosts is limited. We offer new knowledge of morphological and ultrastructural aspects of host-parasite interactions in Myxidium infections from our recent diagnostic investigations on captive freshwater turtles listed in CITES (Appendix II). We investigated the cause of death of 2 adult Indo-Gangetic flap-shelled turtles Lissemys punctata andersonii from a zoo collection. After post-mortem examination, tissues were processed for histopathology, and special stains were used to demonstrate morphology of myxosporean spores. Additional kidney tissue, immersion-fixed in formalin, was processed for transmission electron microscopy. Both turtles were infected with a myxosporidian, Myxidium mackiei, in the kidney, which occluded 5 to 10% of the renal proximal convoluted tubules. The polysporic plasmodia contained pairs of developing and mature spores. Each mature, spindle-shaped spore had 2 asymmetric valves (1 overlapping, 1 overlapped), with 10 to 13 and 10 to 14 longitudinal ridges per valve, and 2 polar capsules each containing a polar filament with 4 to 5 turns. A pair of spores, each surrounded by a membrane-bound electron-lucent matrix, lay in an enclosing cell within the plasmodium. Regions of the parasite-host interface consisted of undulations of the parasite surface, with intense pinocytotic activity beneath, intermingled with the hosts' microvilli, and endocytotic channels at the apex of renal epithelial cells. The microvilli of the renal epithelial cells of infected tubules were frequently sheared or compressed, or occasionally missing; we did not detect other pathology induced by the parasite. Our report of M. mackiei in L. punctata is a new host record. Both individuals also had disseminated

  18. NADPH diaphorase and nitric oxide synthase in the corpus cardiacum-corpus allatum of the cockroach Diploptera punctata.

    Science.gov (United States)

    Skinner, J R; Ren, M; Chan, K K; Stay, B

    2000-01-01

    Juvenile hormone synthesis by corpora allata is regulated partly by allatostatin containing nerves from the brain that innervate the corpora cardiaca and the corpora allata. To investigate whether NO also participates in the regulation of juvenile hormone synthesis, antibody against NO synthase and the histochemical test for NADPH diaphorase activity, a marker for NO synthase, were applied to the corpora cardiaca-corpora allata of Diploptera punctata. Strong NADPH diaphorase activity occurred in corpus allatum cells but not in nerve fibers in the corpora allata or corpora cardiaca. In contrast, NO immunoreactivity occurred in nerves in the corpora cardiaca but not within the corpora allata. NO and allatostatin were not colocalized. NO synthase and NADPH diaphorase activity were localized in similar areas of the subesophageal ganglion and cells in the pars intercerebralis of the brain. Positive correlation of the quantity of NADPH diaphorase activity with juvenile hormone synthesis during the gonadotrophic cycle and lack of such correlation in subesophageal ganglia suggest that NADPH diaphorase activity reflects the necessity of NADPH in the pathway of juvenile hormone synthesis. These data suggest that NO is unlikely to play a significant role in the regulation of the corpora allata. PMID:18088916

  19. Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens.

    Science.gov (United States)

    Jimenez, Cristina M; Sampietro, Diego A; Sgariglia, Melina A; Soberón, José R; Vattuone, Marta A

    2014-10-01

    Infusion, tincture and decoction of leaves of Zuccagnia punctata Cav. were assayed on growth of Fusarium verticillioides, F. graminearum sensu stricto, F. boothii, F. meridionale, F. subglutinans and F. thapsinum. The tincture showed the lowest IC50 on mycelial growth. A diethyl ether fraction of the tincture showed the highest antifungal activity in microdilution assays on F. verticillioides and F. graminearum. The antifungal constituents were separated by silica gel chromatography and identified as 2',4'-dihydroxychalcone, 2',4'-dihydroxy-3'-methoxychalcone and 7-hydroxy-3',4'-dimethoxyflavone. These chalcones had the lowest MIC and MFC values on F. verticillioides and F. graminearum sensu stricto. 2',4'-Dihydroxychalcone was mildly toxic and the remaining identified compounds were non-toxic in the brine shrimp assay. 2',4'-Dihydroxychalcone in mixtures with commercial food preservatives showed additive effects on F. graminearum sensu stricto and synergistic ones on F. verticillioides. 2',4'-Dihydroxy-3'-methoxychalcone showed synergistic effects in mixtures. Our results suggest that addition of chalcones to food preservatives allows reduction in the doses of the preservatives required for control of Fusarium species. PMID:25522536

  20. Histología y química umbral de metales pesados en hígado, branquias y cerebro de Gambusia punctata (Poeciliidae) del río Filé de Santiago de Cuba.

    OpenAIRE

    Argota G.; Larramendi D.; Mora Y.; Argota H.; Fimia R.; Iannacone J.

    2012-01-01

    RESUMENSe realizó un estudio con la especie Gambusia punctata del río Filé de Santiago de Cuba, para establecer niveles de referencia en cuanto a la histología y umbrales de metales pesados en tres órganos diana: hígado, branquias ycerebro. ABSTRACTA study was conducted with the specie Gambusia punctata of Filé river in Santiago de Cuba with the aim of establishing reference values regarding histology and threshold values of heavy metals.

  1. Histología y química umbral de metales pesados en hígado, branquias y cerebro de Gambusia punctata (Poeciliidae del río Filé de Santiago de Cuba.

    Directory of Open Access Journals (Sweden)

    Argota G.

    2012-05-01

    Full Text Available RESUMENSe realizó un estudio con la especie Gambusia punctata del río Filé de Santiago de Cuba, para establecer niveles de referencia en cuanto a la histología y umbrales de metales pesados en tres órganos diana: hígado, branquias ycerebro. ABSTRACTA study was conducted with the specie Gambusia punctata of Filé river in Santiago de Cuba with the aim of establishing reference values regarding histology and threshold values of heavy metals.

  2. Disease: H01194 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available :10391219 Braverman N, Lin P, Moebius FF, Obie C, Moser A, Glossmann H, Wilcox WR, Rimoin DL, Smith M, Kratz...ulfatase. In X-linked dominant CDP (CDPX2), aberrant punctate calcification in cartilage is most prominent around the vertebral co...ngenital disorder characterized by a skeletal abnormality, characterized by punctate calcification of the cartil... L, Kelley RI, Valle D Mutations in the gene encoding 3 beta-hydroxysteroid-delta...plasia punctata (CDPX1); X-linked dominant chondrodysplasia punctata (CDPX2) Chondrodysplasia punctata (CDP) is a co

  3. Frequency of the allelic variant c.1150T > C in exon 10 of the fibroblast growth factor receptor 3 (FGFR3 gene is not increased in patients with pathogenic mutations and related chondrodysplasia phenotypes

    Directory of Open Access Journals (Sweden)

    Thatiane Yoshie Kanazawa

    2014-12-01

    Full Text Available Mutations in the FGFR3 gene cause the phenotypic spectrum of FGFR3 chondrodysplasias ranging from lethal forms to the milder phenotype seen in hypochondroplasia (Hch. The p.N540K mutation in the FGFR3 gene occurs in ~70% of individuals with Hch, and nearly 30% of individuals with the Hch phenotype have no mutations in the FGFR3, which suggests genetic heterogeneity. The identification of a severe case of Hch associated with the typical mutation c.1620C > A and the occurrence of a c.1150T > C change that resulted in a p.F384L in exon 10, together with the suspicion that this second change could be a modulator of the phenotype, prompted us to investigate this hypothesis in a cohort of patients. An analysis of 48 patients with FGFR3 chondrodysplasia phenotypes and 330 healthy (control individuals revealed no significant difference in the frequency of the C allele at the c.1150 position (p = 0.34. One patient carrying the combination `pathogenic mutation plus the allelic variant c.1150T > C' had a typical achondroplasia (Ach phenotype. In addition, three other patients with atypical phenotypes showed no association with the allelic variant. Together, these results do not support the hypothesis of a modulatory role for the c.1150T > C change in the FGFR3 gene.

  4. Determination of Cu, Zn, Pb and Cd by Atomic Emission Spectrometry with Inductively Coupled Plasma in Organs of the Specie Gambusia punctata (Poeciliidae)

    International Nuclear Information System (INIS)

    The aim of this research was to adequate the ICP-AES method to the quantification of Cu, Zn, Pb and Cd in brain, liver and gills of the specie Gambusia punctata, in order to know the level of exposure of the ecosystems San Juan and File in Santiago de Cuba province. To achieve the fitness for purpose of the method, limits of detection, reproducibility and accuracy were evaluated using reference certificated materials of fishes. The biological samples were classified according the ecosystem, length, sex and organ. It were dried, digested with mix of acids and the metals measured in the spectrometer. The results demonstrated that factors as length and sex have not influence in the bioaccumulation, whereas the gill was the organ where all the elements were bioaccumulated. Finally, due that the smallest concentrations of metals were in the File ecosystem, it could be used as an environmental reference

  5. In vitro Charakterisierung der tumor-lytischen Wirkung des Aplysia punctata ink toxins (APIT) auf humane Tumorzellen und Etablierung eines Xenotransplantat-Modells als Basis für in vivo Analysen von APIT

    OpenAIRE

    Brink, Nicola

    2010-01-01

    In vitro characterization of Aplysia punctata ink toxin (APIT) for its anti-cancer efficacy against human cancer cell lines and establishment of xenograft models for in vivo analyzing of APIT The increasing number of patients suffering from different forms of cancer remains a major health problem in industrialized countries. Although commonly used as standard treatment options, surgery, radiation and classical chemotherapy often result in severe adverse reactions, or are even unsuccessful...

  6. Resistência de Haemonchus placei, Cooperia punctata e Oesophagostomum radiatum à ivermectina pour-on a 500mcgkg-1 em rebanhos bovinos no Brasil

    Directory of Open Access Journals (Sweden)

    Welber Daniel Zanetti Lopes

    2014-05-01

    Full Text Available O amplo espectro de ação dos endectocidas e a praticidade de aplicação das suas formulações pour-on são fatores que têm estimulado uma maior frequência de utilização destes produtos na criação de bovinos. Neste estudo, avaliou-se a eficácia anti-helmíntica da ivermectina, administrada via pour-on, 500mcgkg-1, contra nematódeos de bovinos provenientes de diferentes rebanhos. Foram adquiridos 12 bezerros de cada um dos quatro rebanhos selecionados. Todos os 48 bezerros utilizados apresentavam médias de ovos por grama de fezes (OPG maiores que 500, considerando-se três contagens consecutivas. Na sequencia, os animais de cada rebanho foram divididos em dois grupos de seis bovinos cada, sendo um tratado com ivermectina 500mcgkg-1 via pour-on e outro mantido como controle. Quatorze dias depois do tratamento, os bovinos foram submetidos à eutanásia para contagem de endoparasitas. A ivermectina demonstrou resultados nulos de eficácia contra H. placei nos quatro rebanhos. Contra C. punctata, tal formulação teve eficácia nula nos rebanhos provenientes de Jaboticabal,SP, e de Formiga,MG, e eficácias de 75,8% e 58,4% nos rebanhos provenientes de São José do Rio Pardo,SP, e de São Sebastião do Paraíso,MG, respectivamente. Valores de eficácia de 94,2% (Jaboticabal, 0,0% (São José do Rio Pardo, 94,2% (Formiga e 39,2% (São Sebastião do Paraíso foram detectados contra O. radiatum. Com base nos resultados encontrados, pode-se concluir que as quatro populações de Haemonchus placei e de Cooperia punctata avaliadas foram resistentes à ivermectina, administrada via pour-on, 500mcgkg-1. Foram observadas cepas de Oesophagostomum radiatum resistentes à ivermectina em dois dos quatro rebanhos testados.

  7. Expression of anti-tumor necrosis factor alpha (TNFα) single-chain variable fragment (scFv) in Spirodela punctata plants transformed with Agrobacterium tumefaciens.

    Science.gov (United States)

    Balaji, Parthasarathy; Satheeshkumar, P K; Venkataraman, Krishnan; Vijayalakshmi, M A

    2016-05-01

    Therapeutic antibodies against tumor necrosis factor alpha (TNFα) have been considered effective for some of the autoimmune diseases such as rheumatoid arthritis, Crohn's diseases, and so on. But associated limitations of the current therapeutics in terms of cost, availability, and immunogenicity have necessitated the need for alternative candidates. Single-chain variable fragment (scFv) can negate the limitations tagged with the anti-TNFα therapeutics to a greater extent. In the present study, Spirodela punctata plants were transformed with anti-TNFα through in planta transformation using Agrobacterium tumefaciens strain, EHA105. Instead of cefotaxime, garlic extract (1 mg/mL) was used to remove the agrobacterial cells after cocultivation. To the best of our knowledge, this report shows for the first time the application of plant extracts in transgenic plant development. 95% of the plants survived screening under hygromycin. ScFv cDNA integration in the plant genomic DNA was confirmed at the molecular level by PCR. The transgenic protein expression was followed up to 10 months. Expression of scFv was confirmed by immunodot blot. Protein expression levels of up to 6.3% of total soluble protein were observed. β-Glucuronidase and green fluorescent protein expressions were also detected in the antibiotic resistant plants. The paper shows the generation of transgenic Spirodela punctuata plants through in planta transformation. PMID:25786575

  8. Radiographic features of the skeleton in disorders of post-squalene cholesterol biosynthesis

    Energy Technology Data Exchange (ETDEWEB)

    Rossi, Massimiliano; Edery, Patrick [Hospices Civils de Lyon, Genetic Department, Referral Centre for Developmental Abnormalities, Femme-Mere-Enfant Hospital, Bron (France); INSERM U1028 UMR CNRS 5,292, UCBL, CRNL TIGER Team, CH le Vinater, Bron (France); Hall, Christine M. [Retired from Department of Radiology, Great Ormond Street Hospital, London (United Kingdom); Bouvier, Raymonde; Collardeau-Frachon, Sophie [Hospices Civils de Lyon, Department of Pathology, CBPE, Bron (France); Le Breton, Frederique [Hospices Civils de Lyon, Department of Pathology, Croix-Rousse Hospital, Lyon (France); Bucourt, Martine [AP-HP, Foetopathology Unit, Jean Verdier Hospital, Bondy (France); Cordier, Marie Pierre [Hospices Civils de Lyon, Genetic Department, Referral Centre for Developmental Abnormalities, Femme-Mere-Enfant Hospital, Bron (France); Vianey-Saban, Christine [Hospices Civils de Lyon, Department of Inborn Errors of Metabolism and Neonatal Screening, CBPE, Bron (France); Parenti, Giancarlo; Andria, Generoso [Federico II University, Department of Translational Medical Sciences, Section of Pediatrics, Naples (Italy); Le Merrer, Martine [AP-HP, Genetic Department, Referal Centre for Skeletal Dysplasias, Institut Imagine, Necker-Enfants Malades Hospital, Paris (United Kingdom); Offiah, Amaka C. [Stephenson Wing Sheffield Children' s NHS Foundation Trust Western Bank, Radiology Department, Children' s Hospital, Academic Unit of Child Health Room C4, Sheffield (United Kingdom)

    2015-07-15

    Disorders of post-squalene cholesterol biosynthesis are inborn errors of metabolism characterised by multiple congenital abnormalities, including significant skeletal involvement. The most frequent and best-characterised example is the Smith-Lemli-Opitz syndrome. Nine other disorders are known, namely autosomal-recessive Antley-Bixler syndrome, Greenberg dysplasia, X-linked dominant chondrodysplasia punctata, X-linked recessive male emopamil-binding protein deficiency, CHILD syndrome, CK syndrome, sterol C4 methyloxidase-like deficiency, desmosterolosis and lathosterolosis. This study provides an overview of the radiologic features observed in these diseases. A common pattern of limb abnormalities is recognisable, including polydactyly, which is typically post-axial and rarely interdigital and can involve all four limbs, and syndactyly of the toes. Chondrodysplasia punctata is specifically associated with a subgroup of disorders of cholesterol biosynthesis (Greenberg dysplasia, CHILD syndrome, X-linked dominant chondrodysplasia punctata, male emopamil-binding protein deficiency). The possible occurrence of epiphyseal stippling in the Smith-Lemli-Opitz syndrome, initially reported, does not appear to be confirmed. Stippling is also associated with other congenital disorders such as chromosomal abnormalities, brachytelephalangic chondrodysplasia punctata (X-linked recessive chondrodysplasia punctata, disruptions of vitamin K metabolism, maternal autoimmune diseases), rhizomelic chondrodysplasia punctata (peroxisomal disorders) and lysosomal storage disorders. In the differential diagnosis of epiphyseal stippling, a moth-eaten appearance of bones, asymmetry, or presence of a common pattern of limb abnormalities indicate inborn errors of cholesterol biosynthesis. We highlight the specific differentiating radiologic features of disorders of post-squalene cholesterol biosynthesis. (orig.)

  9. Radiographic features of the skeleton in disorders of post-squalene cholesterol biosynthesis

    International Nuclear Information System (INIS)

    Disorders of post-squalene cholesterol biosynthesis are inborn errors of metabolism characterised by multiple congenital abnormalities, including significant skeletal involvement. The most frequent and best-characterised example is the Smith-Lemli-Opitz syndrome. Nine other disorders are known, namely autosomal-recessive Antley-Bixler syndrome, Greenberg dysplasia, X-linked dominant chondrodysplasia punctata, X-linked recessive male emopamil-binding protein deficiency, CHILD syndrome, CK syndrome, sterol C4 methyloxidase-like deficiency, desmosterolosis and lathosterolosis. This study provides an overview of the radiologic features observed in these diseases. A common pattern of limb abnormalities is recognisable, including polydactyly, which is typically post-axial and rarely interdigital and can involve all four limbs, and syndactyly of the toes. Chondrodysplasia punctata is specifically associated with a subgroup of disorders of cholesterol biosynthesis (Greenberg dysplasia, CHILD syndrome, X-linked dominant chondrodysplasia punctata, male emopamil-binding protein deficiency). The possible occurrence of epiphyseal stippling in the Smith-Lemli-Opitz syndrome, initially reported, does not appear to be confirmed. Stippling is also associated with other congenital disorders such as chromosomal abnormalities, brachytelephalangic chondrodysplasia punctata (X-linked recessive chondrodysplasia punctata, disruptions of vitamin K metabolism, maternal autoimmune diseases), rhizomelic chondrodysplasia punctata (peroxisomal disorders) and lysosomal storage disorders. In the differential diagnosis of epiphyseal stippling, a moth-eaten appearance of bones, asymmetry, or presence of a common pattern of limb abnormalities indicate inborn errors of cholesterol biosynthesis. We highlight the specific differentiating radiologic features of disorders of post-squalene cholesterol biosynthesis. (orig.)

  10. qnrVC-Like Gene Located in a Novel Complex Class 1 Integron Harboring the ISCR1 Element in an Aeromonas punctata Strain from an Aquatic Environment in Shandong Province, China▿

    OpenAIRE

    Xia, Ruirui; Guo, Xianhu; Zhang, Yuzhen; Xu, Hai

    2010-01-01

    A qnrVC-like gene, qnrVC4, was found in a novel complex class 1 integron gene cassette array following the ISCR1 element and blaPER-1 in a multidrug-resistant strain of the aquatic bacterium Aeromonas punctata. The deduced QnrVC4 protein sequence shares 45% to 81% amino acid identity with quinolone resistance determinants QnrB6, QnrA1, QnrS1, QnrC, QnrVC1, and QnrVC3. A Ser-83 to Ile amino acid substitution in gyrase A may be mainly responsible for ciprofloxacin resistance in this strain.

  11. qnrVC-like gene located in a novel complex class 1 integron harboring the ISCR1 element in an Aeromonas punctata strain from an aquatic environment in Shandong Province, China.

    Science.gov (United States)

    Xia, Ruirui; Guo, Xianhu; Zhang, Yuzhen; Xu, Hai

    2010-08-01

    A qnrVC-like gene, qnrVC4, was found in a novel complex class 1 integron gene cassette array following the ISCR1 element and bla(PER-1) in a multidrug-resistant strain of the aquatic bacterium Aeromonas punctata. The deduced QnrVC4 protein sequence shares 45% to 81% amino acid identity with quinolone resistance determinants QnrB6, QnrA1, QnrS1, QnrC, QnrVC1, and QnrVC3. A Ser-83 to Ile amino acid substitution in gyrase A may be mainly responsible for ciprofloxacin resistance in this strain. PMID:20516288

  12. In vitro and in vivo plasmalogen replacement evaluations in rhizomelic chrondrodysplasia punctata and Pelizaeus-Merzbacher disease using PPI-1011, an ether lipid plasmalogen precursor

    Directory of Open Access Journals (Sweden)

    Wood Paul L

    2011-10-01

    Full Text Available Abstract Background Childhood peroxisomal disorders and leukodystrophies are devastating diseases characterized by dysfunctional lipid metabolism. Plasmalogens (ether glycerophosphoethanolamine lipids are decreased in these genetic disorders. The biosynthesis of plasmalogens is initiated in peroxisomes but completed in the endoplasmic reticulum. We therefore undertook a study to evaluate the ability of a 3-substituted, 1-alkyl, 2-acyl glyceryl ether lipid (PPI-1011 to replace plasmalogens in rhizomelic chrondrodysplasia punctata type 1 (RCDP1 and rhizomelic chrondrodysplasia punctata type 2 (RCDP2 lymphocytes which possess peroxisomal mutations culminating in deficient plasmalogen synthesis. We also examined plasmalogen synthesis in Pelizaeus-Merzbacher disease (PMD lymphocytes which possess a proteolipid protein-1 (PLP1 missense mutation that results in abnormal PLP1 folding and it's accumulation in the endoplasmic reticulum (ER, the cellular site of the last steps in plasmalogen synthesis. In vivo incorporation of plasmalogen precursor into tissue plasmalogens was also evaluated in the Pex7 mouse model of plasmalogen deficiency. Results In both RCDP1 and RCDP2 lymphocytes, PPI-1011 repleted the target ethanolamine plasmalogen (PlsEtn16:0/22:6 in a concentration dependent manner. In addition, deacylation/reacylation reactions resulted in repletion of PlsEtn 16:0/20:4 in both RCDP1 and RCDP2 lymphocytes, repletion of PlsEtn 16:0/18:1 and PlsEtn 16:0/18:2 in RCDP2 lymphocytes, and partial repletion of PlsEtn 16:0/18:1 and PlsEtn 16:0/18:2 in RCDP1 lymphocytes. In the Pex7 mouse, oral dosing of labeled PPI-1011 demonstrated repletion of tissue levels of the target plasmalogen PlsEtn 16:0/22:6 with phospholipid remodeling also resulting in significant repletion of PlsEtn 16:0/20:4 and PlsEtn 16:0/18:1. Metabolic conversion of PPI-1011 to the target plasmalogen was most active in the liver. Conclusions Our data demonstrate that PPI-1011 is activated

  13. [Taxonomic status of the Burana virus (BURV) (Bunyaviridae, Nairovirus, Tamdy group) isolated from the ticks Haemaphysalis punctata Canestrini et Fanzago, 1877 and Haem. concinna Koch, 1844 (Ixodidae, Haemaphysalinae) in Kyrgyzstan].

    Science.gov (United States)

    L'vov, D K; Al'khovskiĭ, S V; Shchelkanov, M Iu; Shchetinin, A M; Deriabin, P G; Gitel'man, A K; Aristova, V A; Botikov, A G

    2014-01-01

    Complete genome sequence of the Burana virus (BURV) was determined using the next-generation sequencing approach (ID GenBank KF801651). The prototype strain of BURV LEIV-Krg760 was originally isolated from the ticks Haemaphysalis punctata Canestrini et Fanzago, 1877 (Ixodidae, Haemaphysalinae), collected from cows in Tokmak wildlife sanctuary, eastern part of the Chu valley (43 degrees 10' N, 74 degrees 40' E) near Burana village, Kirgizia, in April 1971. Molecular genetics and phylogenetic analyses showed that the BURV belonged to the Nairovirus genus, Bunyaviridae and is related to Tamdy virus (TAMV) that is also associated with the ixodidae ticks of pasture biocenosis in Central Asia. Previous studies showed that TAMV is the prototypic virus of new phylogenetic Tamdy group in the Nairovirus genus. Thus, BURV was classified as a new virus of the Tamdy group, Nairovirus, Bunyaviridae. PMID:25549462

  14. Homeostasis of phospholipids — The level of phosphatidylethanolamine tightly adapts to changes in ethanolamine plasmalogens

    OpenAIRE

    Dorninger, Fabian; Brodde, Alexander; Braverman, Nancy E; Moser, Ann B; Wilhelm W Just; Forss-Petter, Sonja; Brügger, Britta; Berger, Johannes

    2015-01-01

    Ethanolamine plasmalogens constitute a group of ether glycerophospholipids that, due to their unique biophysical and biochemical properties, are essential components of mammalian cellular membranes. Their importance is emphasized by the consequences of defects in plasmalogen biosynthesis, which in humans cause the fatal disease rhizomelic chondrodysplasia punctata (RCDP). In the present lipidomic study, we used fibroblasts derived from RCDP patients, as well as brain tissue from plasmalogen-d...

  15. Neonatal lupus erythematosus associated with unilateral pectoralis major atrophy.

    Science.gov (United States)

    Mondal, Rakesh; Nandi, Madhumita; Sarkar, Sumantra; Mukherjee, Krishnendu

    2011-11-01

    Neonatal lupus erythematosus (NLE), in most cases, presents with cardiac and dermatological manifestation due to transferred IgG auto antibodies (anti Ro/SSA and anti La/SSB) from the mother. Some unusual associations with myelopathy, vasculopathy, transient myasthenia gravis, congenital nephrotic syndrome, chondrodysplasia punctata etc. are also reported. Here, the authors present a case of NLE with isolated left sided pectoralis major muscle atrophy, which has not been reported earlier. PMID:21553209

  16. Cardiopatía, embarazo y tratamiento anticoagulante Anticoagulant therapy in pregnant women with mechanical valve prosthesis and/or heart diseases

    OpenAIRE

    Sandra Braun J

    1999-01-01

    Pregnancy is a hypercoagulable state. Some women with cardiac disease and mechanical valve prosthesis are at increased risk of arterial thromboembolic phenomena. These women are maintained on oral anticoagulants and require effective permanent prophylaxis during pregnancy. The use of oral anticoagulants during pregnancy is controversial because of the risks of embriopathy (Chondrodysplasia punctata) in fetuses who are exposed to coumarin between the 6th and 9th week of gestation, the risk of ...

  17. Functional Neuroanatomy of the Rhinophore of Aplysia punctata

    OpenAIRE

    Rössler Wolfgang; Wertz Adrian; Obermayer Malu; Bickmeyer Ulf

    2006-01-01

    Abstract Background For marine snails, olfaction represents a crucial sensory modality for long-distance reception, as auditory and visual information is limited. The posterior tentacle of Aplysia, the rhinophore, is a chemosensory organ and several behavioural studies showed that the rhinophores can detect pheromones, initiate orientation and locomotion toward food. However the functional neuroanatomy of the rhinophore is not yet clear. Here we apply serotonin-immunohistochemistry and fluore...

  18. Development and physical analysis of YAC contigs covering 7 Mb of Xp22.3-p22.2

    Energy Technology Data Exchange (ETDEWEB)

    Herrell, S.; Novo, F.J.; Charlton, R. [Univ. of Cambridge (United Kingdom)] [and others

    1995-01-20

    A total of YAC clones have been isolated from the region of Xp22.2-p22.3 extending from the amelogenin gene locus to DXS31. Restriction analysis of these clones in association with STS contenting and end clone analysis has facilitated the construction of 6 contigs covering a total of 7 Mb in which 20 potential CpG islands have been located. Thirty new STSs have been developed from probe and YAC end clone sequences, and these have been used in the analysis of patients suffering from different combinations of chondrodysplasia punctata, mental retardation, X-linked ichthyosis, and Kallmann syndrome. The results suggest that (1) the gene for chondrodysplasia punctata must lie between the X chromosome pseudoautosomal boundary (PABX) and DXS1145; (2) a gene for mental retardation lies between DXS1145 and the sequence tagged site GS1; and (3) the gene for ocular albinism type 1 lies proximal to the STS G13. The CpG islands within the YAC contigs constitute valuable markers for the potential positions of genes. Genes found associated with any of these potential CpG islands would be possible candidates for the disease genes mentioned above. 47 refs., 3 figs., 5 tabs.

  19. Hydrops associated with chondrodysplasia of the fetus in a miniature Scottish Highland cow.

    Science.gov (United States)

    Catalina Cabrera, L; McNabb, Bret R; Woods, Sarah E; Cartoceti, Andrew N; Busch, Rosie C

    2016-03-01

    CASE DESCRIPTION A 2-year-old primiparous miniature Scottish Highland cow with an unknown breeding date was evaluated for suspected hydrops. CLINICAL FINDINGS Transabdominal and transrectal ultrasonographic examination identified a large amount of hypoechoic fluid within an enlarged uterus; the fetus could not be identified. Presence of a severely distended uterus and concerns regarding associated health risks to the cow led to the decision to induce labor. Although fluids were expelled, parturition did not progress further over the following 48 hours. Vaginal examination revealed a partially dilated cervix and an abnormally shaped fetus that was too large to pass vaginally. TREATMENT AND OUTCOME Supportive care was provided to the cow, and a stillborn bull calf was delivered by cesarean section. Grossly evident chondrodystrophic dwarfism with hydrocephalus, compatible with so-called bulldog calf malformations, was confirmed by diagnostic imaging and histopathologic evaluation. The cow recovered from surgery uneventfully and was discharged from the hospital the following day. Genetic analysis of DNA from hair roots collected from the sire and dam confirmed both were carriers of an aggrecan-1 gene mutation (bulldog dwarfism1) previously associated with dwarfism and bulldog calf malformations in Dexter cattle. CLINICAL RELEVANCE To our knowledge, this is the first reported case of bulldog calf malformations associated with an aggrecan-1 gene mutation in miniature Scottish Highland cattle, confirming that at least 1 genetic mutation associated with this condition is found in cattle breeds other than Dexter. The findings highlighted the clinical importance of testing for known genetic diseases in breeding cattle, particularly among miniature breeds. PMID:26885599

  20. New insight on FGFR3-related chondrodysplasias molecular physiopathology revealed by human chondrocyte gene expression profiling.

    Directory of Open Access Journals (Sweden)

    Laurent Schibler

    Full Text Available Endochondral ossification is the process by which the appendicular skeleton, facial bones, vertebrae and medial clavicles are formed and relies on the tight control of chondrocyte maturation. Fibroblast growth factor receptor (FGFR3 plays a role in bone development and maintenance and belongs to a family of proteins which differ in their ligand affinities and tissue distribution. Activating mutations of the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia with varying degrees of severity: thanatophoric dysplasia (TD, achondroplasia and hypochondroplasia. Despite progress in the characterization of FGFR3-mediated regulation of cartilage development, many aspects remain unclear. The aim and the novelty of our study was to examine whole gene expression differences occurring in primary human chondrocytes isolated from normal cartilage or pathological cartilage from TD-affected fetuses, using Affymetrix technology. The phenotype of the primary cells was confirmed by the high expression of chondrocytic markers. Altered expression of genes associated with many cellular processes was observed, including cell growth and proliferation, cell cycle, cell adhesion, cell motility, metabolic pathways, signal transduction, cell cycle process and cell signaling. Most of the cell cycle process genes were down-regulated and consisted of genes involved in cell cycle progression, DNA biosynthesis, spindle dynamics and cytokinesis. About eight percent of all modulated genes were found to impact extracellular matrix (ECM structure and turnover, especially glycosaminoglycan (GAG and proteoglycan biosynthesis and sulfation. Altogether, the gene expression analyses provide new insight into the consequences of FGFR3 mutations in cell cycle regulation, onset of pre-hypertrophic differentiation and concomitant metabolism changes. Moreover, impaired motility and ECM properties may also provide clues about growth plate disorganization. These results also suggest that many signaling pathways may be directly or indirectly altered by FGFR3 and confirm the crucial role of FGFR3 in the control of growth plate development.

  1. Comparative transcriptome analysis to investigate the high starch accumulation of duckweed (Landoltia punctata) under nutrient starvation

    OpenAIRE

    Tao, Xiang; Fang, Yang; Xiao, Yao; Jin, Yan-Ling; Ma, Xin-Rong; Zhao, Yun; He, Kai-ze; Zhao, Hai; Wang, Hai-Yan

    2013-01-01

    Background Duckweed can thrive on anthropogenic wastewater and produce tremendous biomass production. Due to its relatively high starch and low lignin percentage, duckweed is a good candidate for bioethanol fermentation. Previous studies have observed that water devoid of nutrients is good for starch accumulation, but its molecular mechanism remains unrevealed. Results This study globally analyzed the response to nutrient starvation in order to investigate the starch accumulation in duckweed ...

  2. Succinic acid production from duckweed (Landoltia punctata) hydrolysate by batch fermentation of Actinobacillus succinogenes GXAS137.

    Science.gov (United States)

    Shen, Naikun; Wang, Qingyan; Zhu, Jing; Qin, Yan; Liao, Siming; Li, Yi; Zhu, Qixia; Jin, Yanling; Du, Liqin; Huang, Ribo

    2016-07-01

    Duckweed is potentially an ideal succinic acid (SA) feedstock due to its high proportion of starch and low lignin content. Pretreatment methods, substrate content and nitrogen source were investigated to enhance the bioconversion of duckweed to SA and to reduce the costs of production. Results showed that acid hydrolysis was an effective pretreatment method because of its high SA yield. The optimum substrate concentration was 140g/L. The optimum substrate concentration was 140g/L. Corn steep liquor powder could be considered a feasible and inexpensive alternative to yeast extract as a nitrogen source. Approximately 57.85g/L of SA was produced when batch fermentation was conducted in a 1.3L stirred bioreactor. Therefore, inexpensive duckweed can be a promising feedstock for the economical and efficient production of SA through fermentation by Actinobacillus succinogenes GXAS137. PMID:27023386

  3. Prey refuges as predator hotspots: ocelot (Leopardus pardalis) attraction to agouti (Dasyprocta punctata) dens

    NARCIS (Netherlands)

    Emsens, W.J.; Hirsch, B.T.; Kays, R.; Jansen, P.A.

    2014-01-01

    We tested the hypothesis that prey refuges attract predators, leading to elevated predator activity in the vicinity of refuges. We used camera traps to determine whether the spatial activity of a predator, the ocelot (Leopardus pardalis), was biased toward refuge locations of its principal prey, the

  4. Synthesis and bioactivity of novel caffeic acid esters from Zuccagnia punctata.

    Science.gov (United States)

    Ramachandra, M S; Subbaraju, G V

    2006-12-01

    Synthesis of novel caffeic acid esters (1 and 2) was accomplished starting from appropriately substituted benzaldehydes (3 and 9). While compound 2 exhibited potent anti-oxidative activity in both the nitroblue tetrazolium and 1,1-diphenyl-2-picrylhydrazyl radical-scavenging models, compound 1 showed moderate 5-lipoxygenase inhibitory activity. PMID:17145655

  5. Targeted overexpression of parathyroid hormone-related peptide in chondrocytes causes chondrodysplasia and delayed endochondral bone formation.

    OpenAIRE

    Weir, E C; Philbrick, W M; Amling, M.; Neff, L A; Baron, R; Broadus, A E

    1996-01-01

    Parathyroid hormone-related peptide (PTHrP) was initially identified as a product of malignant tumors that mediates paraneoplastic hypercalcemia. It is now known that the parathyroid hormone (PTH) and PTHrP genes are evolutionarily related and that the products of these two genes share a common receptor, the PTH/PTHrP receptor. PTHrP and the PTH/PTHrP receptor are widely expressed in both adult and fetal tissues, and recent gene-targeting and disruption experiments have implicated PTHrP as a ...

  6. [Genodermatosis in man and animal. Comparative overview].

    Science.gov (United States)

    Küster, W; Happle, R

    1983-11-01

    Fourteen monogenic cutaneous disorders of man are compared to similar gene defects in animals. The traits are classified into two groups. In the first group, an identity (homology) of the underlying gene defects is likely. This group includes oculo-cutaneous albinism, Chédiak-Higashi syndrome, aplasia cutis congenita, Ehlers-Danlos syndrome (type I), hypohidrotic ectodermal dysplasia of the Christ-Siemens-Touraine type, X-linked dominant chondrodysplasia punctata, ichthyosis congenita gravis, Menkes syndrome, erythropoetic porphyria, porphyria cutanea tarda, and acrodermatitis enteropathica. In the second group, the traits are similar but the question of their homology cannot be settled. It includes alopecia congenita, hidrotic ectodermal dysplasia of the Clouston type, and hereditary lymphedema. The existence of identical mutations in man and animals provides evidence for the close relationship between the various mammalian species. Homologous traits affecting the skin are of practical importance since the use of these animal models may help to answer those questions which cannot be answered by performing research in human patients. PMID:6358130

  7. Autoimmune diseases in pregnancy: maternal and fetal outcomes

    Directory of Open Access Journals (Sweden)

    Pavithra M. Vengetesh

    2015-02-01

    Full Text Available Background: The aim of this study was to assess the impact of autoimmune connective tissue disorders on the outcomes of pregnancy and the influence of treatment on pregnancy. Methods: Thirty-seven antenatal patients with autoimmune connective tissue diseases, comprising of Systemic Lupus Erythematosus (SLE, primary antiphospholipid antibody syndrome (APS, Mixed Connective Tissue Diseases (MCTD, ankylosing spondylitis and Takayasu arteritis were analysed. Results: Multigravidas constituted 89.4% and were associated with bad obstetric history. Before diagnosis and treatment, serious maternal complications of eclampsia and thromboembolism were observed in patients with SLE and APS. The live birth rates were 9% and 2.4% respectively in patients with SLE and APS. With appropriate treatment- aspirin, heparin and immunosuppressant, the live birth rates were raised to 70% in SLE and 100% in APS patients. Investigation for autoimmune disease in recurrent pregnancy loss is important. A rare association between MCTD and congenital anomaly - Rhizomelic chondrodysplasia punctata was observed. Preeclampsia, gestational diabetes, fetal growth restriction and preterm labour were the common complications noted. Conclusions: Active disease at onset of pregnancy, presence of Anti-ds DNA antibodies and secondary APS were strong predictors of poor pregnancy outcomes among patients with SLE. Vigilant monitoring during pregnancy is required for favourable outcomes. [Int J Reprod Contracept Obstet Gynecol 2015; 4(1.000: 9-14

  8. The ether lipid-deficient mouse: tracking down plasmalogen functions.

    Science.gov (United States)

    Gorgas, Karin; Teigler, Andre; Komljenovic, Dorde; Just, Wilhelm W

    2006-12-01

    Chemical and physico-chemical properties as well as physiological functions of major mammalian ether-linked glycerolipids, including plasmalogens were reviewed. Their chemical structures were described and their effect on membrane fluidity and membrane fusion discussed. The recent generation of mouse models with ether lipid deficiency offered the possibility to study ether lipid and particularly plasmalogen functions in vivo. Ether lipid-deficient mice revealed severe phenotypic alterations, including arrest of spermatogenesis, development of cataract and defects in central nervous system myelination. In several cell culture systems lack of plasmalogens impaired intracellular cholesterol distribution affecting plasma membrane functions and structural changes of ER and Golgi cisternae. Based on these phenotypic anomalies that were accurately described conclusions were drawn on putative functions of plasmalogens. These functions were related to cell-cell or cell-extracellular matrix interactions, formation of lipid raft microdomains and intracellular cholesterol homeostasis. There are several human disorders, such as Zellweger syndrome, rhizomelic chondrodysplasia punctata, Alzheimer's disease, Down syndrome, and Niemann-Pick type C disease that are distinguished by altered tissue plasmalogen concentrations. The role plasmalogens might play in the pathology of these disorders is discussed. PMID:17027098

  9. Ectopic bone formation and chondrodysplasia in transgenic mice carrying the rat C3(1)/T{sub AG} fusion gene

    Energy Technology Data Exchange (ETDEWEB)

    Green, J.E.; Maroulakou, I.G.; Anver, M. [National Cancer Institute, Frederick, MD (United States)] [and others

    1994-09-01

    Transgenic mice expressing the SV40 large T-antigen (T{sup AG}) under the regultory control of the hormone-responsive rat C3(1) prostatein promoter develop unusual bone and cartilage lesions, as well as ectopic bone and cartilage formation. Two lines of transgenic animals have been propagated in which the expression of the transgene in chondrocytes results in a mild to moderate generalized disorganization of cartilage growth which appears to affect multiple tissues, including the trachea, ear pinna and articular cartilage. The epiphyseal plates are also affected with normal architecture of the zones of proliferation and maturation, but marked elongation of the zone of hypertrophy. Immunocytochemistry demonstrates that expression of T{sup AG} is limited to the zone of hypertropny in the epiphyseal plates, suggesting that the chondrocytes become hormone-responsive at this particular stage of differentiation. Normal mineralization and trabecular formation in long bone appears to occur. Ectopic bone and cartilage formation occurs in the foot pads of the fore- and hind- feet over the course of several months. This is preceded by proliferation of sweat gland epithelial cells followed by the appearance of nodules of cartilage and bone. The nodules are closely associated with proliferating epithelium but are not contiguous with bony structures normally found in the feet. The roles of BMP`s, growth factors, oncogenes and hormones in the development of these lesions will be presented. These transgenic animals may provide new insights into hormone-responsiveness of chondrocytes, as well as factors involved in the processes of bone and cartilage differentiation and growth. These transgenic animals may serve as a useful model for human heterotopic bone formation.

  10. Impaired neurotransmission in ether lipid-deficient nerve terminals

    Science.gov (United States)

    Brodde, Alexander; Teigler, Andre; Brugger, Britta; Lehmann, Wolf D.; Wieland, Felix; Berger, Johannes; Just, Wilhelm W.

    2016-01-01

    Isolated defects of ether lipid (EL) biosynthesis in humans cause rhizomelic chondrodysplasia punctata type 2 and type 3, serious peroxisomal disorders. Using a previously described mouse model [Rodemer, C., Thai, T.P., Brugger, B., Kaercher, T., Werner, H., Nave, K.A., Wieland, F., Gorgas, K., and Just, W.W. (2003) Inactivation of ether lipid biosynthesis causes male infertility, defects in eye development and optic nerve hypoplasia in mice. Hum. Mol. Genet., 12, 1881–1895], we investigated the effect of EL deficiency in isolated murine nerve terminals (synaptosomes) on the pre-synaptic release of the neurotransmitters (NTs) glutamate and acetylcholine. Both Ca2+-dependent exocytosis and Ca2+-independent efflux of the transmitters were affected. EL-deficient synaptosomes respire at a reduced rate and exhibit a lowered adenosin-5′-triphosphate/adenosine diphosphate (ATP/ADP) ratio. Consequently, ATP-driven processes, such as synaptic vesicle cycling and maintenance of Na+, K+ and Ca2+ homeostasis, might be disturbed. Analyzing reactive oxygen species in EL-deficient neural and non-neural tissues revealed that plasmalogens (PLs), the most abundant EL species in mammalian central nervous system, considerably contribute to the generation of the lipid peroxidation product malondialdehyde. Although EL-deficient tissue contains less lipid peroxidation products, fibroblasts lacking ELs are more susceptible to induced oxidative stress. In summary, these results suggest that due to the reduced energy state of EL-deficient tissue, the Ca2+-independent efflux of NTs increases while the Ca2+-dependent release declines. Furthermore, lack of PLs is mainly compensated for by an increase in the concentration of phosphatidylethanolamine and results in a significantly lowered level of lipid peroxidation products in the brain cortex and cerebellum. PMID:22403185

  11. Disease: H00445 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H00445 Osteoarthritis with mild chondrodysplasia The disease is characterized by a ...M: 604864 PMID:11708863 (description, gene) Mier RJ, Holderbaum D, Ferguson R, Moskowitz R Osteoarthritis...ymorphism in the type II procollagen gene (COL2A1) to primary osteoarthritis associated with mild chondrodysplasia. N Engl J Med 322:526-30 (1990) ...

  12. The influence of duckweed species diversity on biomass productivity and nutrient removal efficiency in swine wastewater.

    Science.gov (United States)

    Zhao, Zhao; Shi, Huijuan; Liu, Yang; Zhao, Hai; Su, Haifeng; Wang, Maolin; Zhao, Yun

    2014-09-01

    The effect of temperature, light intensity, nitrogen and phosphorus concentrations on the biomass and starch content of duckweed (Landoltia punctata OT, Lemna minor OT) in monoculture and mixture were assessed. Low light intensity promoted more starch accumulation in mixture than in monoculture. The duckweed in mixture had higher biomass and nutrient removal efficiency than those in monoculture in swine wastewater. Moreover, the ability of L. punctata C3, L. minor C2, Spirodela polyrhiza C1 and their mixtures to recovery nutrients and their biomass were analyzed. Results showed that L. minor C2 had the highest N and P content, while L. punctata C3 had the highest starch content, and the mixture of L. punctata C3 and L. minor C2 had the greatest nutrient removal rate and the highest biomass. Compared with L. punctata C3 and L. minor C2 in monoculture, their biomass in mixture increased by 17.0% and 39.8%, respectively. PMID:24998479

  13. Disease: H00493 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available wicz M Chondrodysplasias due to proteoglycan defects. Glycobiology 12:57R-68R (20... Carson DD Heparan sulfate proteoglycans: key players in cartilage biology. Crit Rev Eukaryot Gene Expr 15:2

  14. Espèce nouvelle du genre Necrophorus de l’Archipel Indo-Néerlandais

    NARCIS (Netherlands)

    Grouvelle, A.

    1893-01-01

    Niger; antennarum tribus ultimis articulis, elytrorum epipleuris et duabus fasciis transversis aurantiacis; prima fascia subbasali, humeros attingente et suturam versus interrupta, infra humeros nigro-punctata; secunda fascia subapicali, flexuosa, suturam lateraque haud attingente; prothorace transv

  15. Parasitism, commensalism or mutualism? The case of Scyphozoa (Coronatae) and horny sponges

    OpenAIRE

    Uriz, María Jesús; Rosell, Dolors; Maldonado, Manuel

    1992-01-01

    The relationship between the scyphozoan Nausitoe punctata and the horny sponges Cacospongia scalans, Dysidea avara and D. fragdis was analyzed. Evidence was found for utilization of the thecae of N. punctata as a substitute for skeletal fibres. Consequently some metabolic costs associated with skeleton-buildng may be reduced for the sponge. Further potential benefits for the scyphozoan, e.g. protection against predation and mechanical disturbance, and trophic advantages, are di...

  16. Disease: H00515 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H00515 The DTDST-related disorders, including: Achondrogenesis IB (ACG IB); Atelost...eogenesis II (AO II); Diastrophic dysplasia (DTD) The DTDST-related disorders are severe recessive chondrody...splasias including two lethal disorders ACG IB and AO II; and a non-lethal disorder DTD. Patients with these disorders

  17. Disease: H00468 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available genital anomalies is a condition that shows severe limb malformation with brachydactyly. Hypogonadism is also present. It is cause...Lehmann K A homozygous BMPR1B mutation causes a new subtype of acromesomelic chondrodysplasia with genital a

  18. A taxonomic study of the beetle cockroaches (Diploptera Saussure) from China, with notes on the genus and species worldwide (Blattodea: Blaberidae: Diplopterinae).

    Science.gov (United States)

    Li, Xinran; Wang, Zongqing

    2015-01-01

    Four taxa of beetle cockroaches (Diploptera Saussure, 1864) from South China are described and illustrated, viz., two new species D. elliptica sp. n. and D. naevus sp. n., one new subspecies D. nigrescens guani subsp. n. and one widespread known species D. punctata (Eschscholtz, 1822). The genus and known species from around the world are discussed based on types and other specimens. D. pulchra Anisyutkin, 2007 is now regarded as a junior synonym of D. bicolor Hanitsch, 1925. Whether the populations of D. punctata represent or not different species needs to be studied in the future. A key, a distribution map, and photos of species are provided. PMID:26624027

  19. fRNAdb Summary: FR291009 [

    Lifescience Database Archive (English)

    Full Text Available FR291009 AF198362,AF198367,AF198369,AF198370,AF198371,AF198372,AF198373,AF198375,AF198377,AF1983 ... mirabilis,Providencia stuartii,Edwardsiella tarda,Aeromonas ... salmonicida,Aeromonas ... punctata,Aeromonas ... veronii,V ... ibrio alginolyticus,Edwardsiella ictaluri,Aeromonas ... veronii bv. sobria,Photorhabdus luminescens subsp. ...

  20. fRNAdb Summary: FR217426 [

    Lifescience Database Archive (English)

    Full Text Available FR217426 AB003402,AB035920,AB035923,AB035926,AE005174,AE005674,AE008706,AE008886,AE008893,AE0167 ... igella boydii,Shigella flexneri,Edwardsiella tarda,Aeromonas ... hydrophila,Aeromonas ... punctata,Aeromonas ... veronii,Pr ... almonella enterica subsp. enterica serovar Newport,Aeromonas ... veronii bv. sobria,Salmonella enterica subsp. ente ...

  1. fRNAdb Summary: FR152950 [

    Lifescience Database Archive (English)

    Full Text Available FR152950 AY907109,AY907112,AY907113,AY907117,AY907120,AY907125,AY907127 5.8S ribosomal RNA (rRNA ... ala,Malaxis metallica,Malaxis punctata,Malaxis sp. Fiji ,Malaxis taurina RF00002 Rfam v8.1 FR152950.jpg FR1 ...

  2. Site records of softshell turtles (Chelonia: Trionychidae from Barak Valley, Assam, northeastern India

    Directory of Open Access Journals (Sweden)

    K.C. Das

    2011-04-01

    Full Text Available We report for the first time the occurrence of four species of Trionychid turtles Nilssonia gangetica, N. hurum, Chitra indica and Lissemys punctata andersonii from 57 sites in the Barak Valley region of Assam, northeastern India. Sites of occurrence include rivers, small streams, floodplain lakes and ox-bows.

  3. Espécies novas e chave para as espécies de Callia (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Galileo Maria Helena M.

    2002-01-01

    Full Text Available New species described: Callia marginata from Peru, C. punctata from Colombia, C. annulata from Ecuador, C. tristis from Bolivia, C. paraguaya from Paraguay; from Brazil: C. divisa and C. tomentosa (Mato Grosso, and C. lissonota (Rondônia. A key to the species of Callia is added.

  4. The South American Mailed Catfishes of the genus Pseudoloricaria Bleeker, 1862 (Pisces, Siluriformes, Loricariidae)

    NARCIS (Netherlands)

    Isbrücker, I.J.H.; Nijssen, H.

    1976-01-01

    Two species of South American Mailed Catfishes of the genus Pseudoloricaria Bleeker, 1862 are redescribed and figured from type-specimens and additional material: Pseudoloricaria laeviuscula (Valenciennes, 1840), and Pseudoloricaria punctata (Regan, 1904). Since the provenance of the holotype of P.

  5. Congrid eels of the Eastern Pacific and key to their leptocephali

    OpenAIRE

    Raju, Solomon N.

    1985-01-01

    This study indicates that 13 species of congrid larvae belonging to 8 genera occur in the eastern Pacific. The species are: Ariosoma gilberti; Paraconger californiensis; Paraconger sp.; P. dentatus; Chiloconger labiatus; Taenioconger digueti; T. canabus; Gorgasia punctata; G. obtusa; Gnathophis catalinensis; Hildebrandia nitens; Bathycongrus macrurus; and B. varidens. The morphological and anatomical changes undergone during metamorphosis are useful in the identification of the larva...

  6. Seed predation and defleshing in the agouti-dispersed palm Astrocaryum standleyanum

    NARCIS (Netherlands)

    Jansen, P.A.; Elschot, K.; Verkerk, P.J.; Wright, S.J.

    2010-01-01

    The agouti (Dasyprocta punctata) meticulously defleshes Astrocaryum standleyanum palm seeds before scatter hoarding. On Barro Colorado Island, Panama, we experimentally tested three hypotheses on how this behaviour could reduce seed predation to the mutual benefit of the tree and the rodent. The fir

  7. Ellis-van Creveld syndrome in an Indian child: a case report

    OpenAIRE

    K M Veena; Jagadishchandra, H; Rao, Prasanna Kumar; Chatra, Laxmikanth

    2011-01-01

    Ellis-van Creveld syndrome is a rare congenital genetic disorder having autosomal recessive inheritance. It is a syndrome affecting the Amish population of Pennsylvania in USA with prevalence rate of 1/5,000 live at birth. In non-Amish population, the birth prevalence is 7/1,000,000. The syndrome is characterized by bilateral postaxial polydactyly of the hands, chondrodysplasia of long bones resulting in acromesomelic dwarfism, ectodermal dysplasia affecting nails as well as teeth and congeni...

  8. Profilin 1 is required for abscission during late cytokinesis of chondrocytes

    OpenAIRE

    Böttcher, Ralph T.; Wiesner, Sebastian; Braun, Attila; Wimmer, Reiner; Berna, Alejandro; Elad, Nadav; Medalia, Ohad; Pfeifer, Alexander; Aszódi, Attila; Costell, Mercedes; Fässler, Reinhard

    2009-01-01

    Profilins are key factors for dynamic rearrangements of the actin cytoskeleton. However, the functions of profilins in differentiated mammalian cells are uncertain because profilin deficiency is early embryonic lethal for higher eukaryotes. To examine profilin function in chondrocytes, we disrupted the profilin 1 gene in cartilage (Col2pfn1). Homozygous Col2pfn1 mice develop progressive chondrodysplasia caused by disorganization of the growth plate and defective chondrocyte cytokinesis, indic...

  9. Identification of a Novel Mutation in the COL2A1 Gene in a Chinese Family with Spondyloepiphyseal Dysplasia Congenita

    OpenAIRE

    Huang, Xiangjun; Deng, Xiong; Xu, Hongbo; Wu, Song; Yuan, Lamei; Yang, Zhijian; Yang, Yan; DENG, HAO

    2015-01-01

    Spondyloepiphyseal dysplasia congenita (SEDC) is an autosomal dominant chondrodysplasia characterized by disproportionate short-trunk dwarfism, skeletal and vertebral deformities. Exome sequencing and Sanger sequencing were performed in a Chinese Han family with typical SEDC, and a novel mutation, c.620G>A (p.Gly207Glu), in the collagen type II alpha-1 gene (COL2A1) was identified. The mutation may impair protein stability, and lead to dysfunction of type II collagen. Family-based study sugge...

  10. Spondyloepiphyseal dysplasia congenita: genetic linkage to type II collagen (COL2AI).

    OpenAIRE

    Anderson, I J; Goldberg, R.B.; Marion, R W; Upholt, W B; Tsipouras, P

    1990-01-01

    Spondyloepiphyseal dysplasia congenita (SEDC) is an autosomal dominantly inherited chondrodysplasia characterized by disproportionate short stature (short trunk), abnormal epiphyses, and flattened vertebral bodies. Manifestations are present at birth. We ascertained a 4-generation family exhibiting the clinical manifestations of the disorder. Previous evidence suggesting defects of type II collagen associated with the SEDC phenotype led us to genotype the family for various COL2A1 gene-associ...

  11. TCTEX1D2 mutations underlie Jeune asphyxiating thoracic dystrophy with impaired retrograde intraflagellar transport

    OpenAIRE

    Kayserili Karabey, Hülya; Schmidts, Miriam; Hou, Yuqing; Cortes, Claudio R.; Mans, Dorus A.; Huber, Celine; Boldt, Karsten; Patel, Mitali; van Reeuwijk, Jeroen; Plaza, Jean-Marc; van Beersum, Sylvia E. C.; Yap, Zhi Min; Letteboer, Stef J. F.; Taylor, S. Paige; Herridge, Warren; Johnson, Colin A.; Scambler, Peter J.; Ueffing, Marius; Krakow, Deborah; King, Stephen M.; Beales, Philip L.; Al-Gazali, Lihadh; Wicking, Carol; Cormier-Daire, Valerie; Roepman, Ronald; Mitchison, Hannah M.; Witman, George B.

    2015-01-01

    The analysis of individuals with ciliary chondrodysplasias can shed light on sensitive mechanisms controlling ciliogenesis and cell signalling that are essential to embryonic development and survival. Here we identify TCTEX1D2 mutations causing Jeune asphyxiating thoracic dystrophy with partially penetrant inheritance. Loss of TCTEX1D2 impairs retrograde intraflagellar transport (IFT) in humans and the protist Chlamydomonas, accompanied by destabilization of the retrograde IFT dynein motor. W...

  12. Shwachman-Diamond syndrome: first molecular diagnosis in a Brazilian child

    OpenAIRE

    Cresio Alves; Julia Constanca Fernandes; Silvana Sampaio; Raquel de Melo Alves Paiva; Rodrigo Tocantins Calado

    2013-01-01

    Herein the first molecular diagnosis of a Brazilian child with Shwachman-Diamond Syndrome is reported. A 6-year-old boy was diagnosed with cystic fibrosis at the age of 15 months due to recurrent respiratory infections, diarrhea and therapeutic response to pancreatic enzymes. Three sweat tests were negative. At the age of 5 years, he began to experience pain in the lower limbs, laxity of joints, lameness and frequent falls. A radiological study revealed metaphyseal chondrodysplasia. A complet...

  13. Dwarfism and gigantism in historical picture postcards.

    OpenAIRE

    Enderle, A

    1998-01-01

    A collection of 893 historical picture postcards from 1900 to 1935, depicting dwarfs and giants, was analysed from medical and psychosocial viewpoints. In conditions such as 'bird headed dwarfism', achondroplasia, cretinism, so-called Aztecs or pinheads, Grebe chondrodysplasia, and acromegalic gigantism, the disorder could be diagnosed easily. In hypopituitary dwarfism, exact diagnosis was more difficult because of heterogeneity. The most common conditions depicted were pituitary dwarfism and...

  14. Achondroplasia Presenting with Pneumonia in a Two Months Old Boy

    OpenAIRE

    Huseyin Bilgin

    2014-01-01

    Achondroplasia is one of the common chondrodysplasias with an inheritance is autosomal dominant, but in around 85% the phenotype is the result of a new mutation. Achondroplasia develops as a result of dysplasia of enchondral formation due to the mutation of fibroblast growth factor receptor 3. A 2-month-old boy was referred to the our hospital with cough and fever. Craniofacially the head appeared large and also frontal bossing and depressed nasal bridge was demonstrated. Narrow lumbar interp...

  15. Den molekylaergenetiske baggrund for en raekke arvelige kraniosynostoser og kondrodysplasier

    DEFF Research Database (Denmark)

    Hertz, Jens Michael; juncker, Inger; Christensen, L; Østergaard, J R; Jensen, P K

    2001-01-01

    Fibroblast growth factors are structurally related proteins associated with cell growth, differentiation, migration, wound healing, angiogenesis, and oncogenesis. At the cellular level, their function is mediated by transmembrane tyrosinekinase receptors, fibroblast growth factor receptors. Four ......, ACS. The same mutation can cause different syndromes, and the same syndrome can be caused by mutations in different genes. The chondrodysplasias: achondroplasia, hypochondroplasia, and thanatophoric dysplasia are all caused by mutations in FGFR3....

  16. Exome sequencing identifies DYNC2H1 mutations as a common cause of asphyxiating thoracic dystrophy (Jeune syndrome) without major polydactyly, renal or retinal involvement.

    OpenAIRE

    Schmidts, M.; Arts, H. H.; Bongers, E.M.H.F.; Yap, Z.; Oud, M. M.; Antony, D.; Duijkers, L.; Emes, R. D.; Stalker, J.; Yntema, J.-B. L.; Plagnol, V.; Hoischen, A.; Gilissen, C; Forsythe, E.; Lausch, E.

    2013-01-01

    BACKGROUND: Jeune asphyxiating thoracic dystrophy (JATD) is a rare, often lethal, recessively inherited chondrodysplasia characterised by shortened ribs and long bones, sometimes accompanied by polydactyly, and renal, liver and retinal disease. Mutations in intraflagellar transport (IFT) genes cause JATD, including the IFT dynein-2 motor subunit gene DYNC2H1. Genetic heterogeneity and the large DYNC2H1 gene size have hindered JATD genetic diagnosis. AIMS AND METHODS: To determine the contribu...

  17. Articular cartilage collagen: an irreplaceable framework?

    OpenAIRE

    Eyre, D. R.; Weis, M A; J-J Wu

    2006-01-01

    Adult articular cartilage by dry weight is two-thirds collagen. The collagen has a unique molecular phenotype. The nascent type II collagen fibril is a heteropolymer, with collagen IX molecules covalently linked to the surface and collagen XI forming the filamentous template of the fibril as a whole. The functions of collagens IX and XI in the heteropolymer are far from clear but, evidently, they are critically important since mutations in COLIX and COLXI genes can result in chondrodysplasia ...

  18. Frequency and intensity of infection by helminths in cattle slaughtered at the abattior, of the northwest of region state of São Paulo, Brazil/ Freqüência e intensidade parasitária de helmintos gastrintestinais em bovinos abatidos em frigorífico da região noroeste do Estado de São Paulo, SP, Brasil

    Directory of Open Access Journals (Sweden)

    Luis Gustavo Ferraz Lima

    2001-08-01

    Full Text Available Parasitary gastrointestinal helminths frequency and intensity were studied in 48 female bovines, with different zebu and taurine crossbreeding degrees, ages ranging between 24 and 30 months, from eight counties in the Northwest of São Paulo State, slaughtered at Frigorífico Montenegro, Araçatuba, São Paulo (Brazil and four animals were monthly necropsied over one year. The identified gastrointestinal helminth species were: Cooperia punctata, Cooperia pectinata, Haemonchus similis, Haemonchus placei and Oesophagostomum radiatum. Among the 48 animals, 21 (43,8% were carrying Haemonchus spp. The Haemonchus similis and the Haemonchus placei species were identified isolated at an equivalent rate (10,4%, being simultaneous infection registered in 22,9% of the cases. In small intestine, 26 bovines (54,2% showed Cooperia punctata. Ten male Cooperia pectinata were identified in only one bovine that also carried 310 Cooperia punctata. In the large intestine, 20 bovines (41,7% showed Oesophagostomum radiatum. Mixed infections occurred in 52,1% of the animals, simple infections in 12%, and 22,9% of the bovines did not have gastrointestinal helminths.A freqüência e a intensidade parasitária de helmintos gastrintestinais foram estudadas em 48 bovinos, fêmeas, com diferentes graus de cruzamento de raças zebuínas e taurinas, de faixa etária entre 24 a 30 meses, provenientes de oito municípios da região noroeste do Estado de São Paulo e abatidos no Frigorífico Montenegro, Araçatuba, SP, sacrificando-se quatro animais mensalmente, ao longo de um ano. As espécies de helmintos identificadas foram: Cooperia punctata, Cooperia pectinata, Haemonchus similis, Haemonchus placei e Oesophagostomum radiatum. Dos animais examinados, 21 (43,8% estavam parasitados por Haemonchus spp. As espécies H. similis e H. placei foram identificadas isoladamente em igual proporção (10,4% em dez bovinos, sendo que a infecção simultânea foi registrada em outros 11

  19. fRNAdb Summary: FR138127 [

    Lifescience Database Archive (English)

    Full Text Available FR138127 AF198361,AF198363,AF198366,AF198374,AF198376,AF198379,AF407225,AF407231,AJ535850,AJ5358 ... 4 transfer RNA (tRNA), GAT (Ile/I) Isoleucine tRNA Aeromonas ... punctata,Aeromonas ... veronii,Photobacterium damselae ... piscicida,Photobacterium damselae subsp. damselae,Aeromonas ... veronii bv. sobria RF00005 Rfam v8.1 FR138127.jpg ...

  20. Abilities of some higher plants to hydrolyze the acetates of phenols and aromatic-aliphatic alcohols

    OpenAIRE

    Agnieszka Mironowicz; Krystyna Kromer; Paweł Pawłowicz; Antoni Siewiński

    2014-01-01

    In the biotransformations carried out under the same conditions, the whole intact plants of Spirodela punctata, Nephrolepis exaltata, Cyrtomium falcatum, Nephrolepis cordifolia and the suspension cultures of Helianthus tuberosus, Daucus carota and Petunia hybrida hydrolyze (partially or totally) the ester bonds of the acetates of phenols and aromatic-aliphatic alcohols and also the menthyl acetate. Nevertheless, the methyl esters of aromatic acids, structurally similar to the former substrate...

  1. Detection of Theileria luwenshuni in sheep from Great Britain

    OpenAIRE

    Phipps, L. Paul; Hernández-Triana, Luis M.; Goharriz, Hooman; Welchman, David; Johnson, Nicholas

    2016-01-01

    Background Theileria spp. are tick-borne protozoan parasites of the Phylum Apicomplexa, Order Piroplasmida that infect a wide range of wild and domestic animals. In Great Britain, Theileria spp. have been reported from livestock associated with transmission by the tick Haemaphysalis punctata. However, these reports have not been associated with disease. This study has investigated the cause of a disease outbreak accompanied by mortality in a flock of sheep grazing reclaimed marshland in north...

  2. Gastro-intestinal nematodes and cestodes of cattle in Burkina Faso

    OpenAIRE

    Belem A.M.G.; Ouedraogo O.P.; Bessin R.

    2001-01-01

    A survey of the parasites of abomasa, small, and large intestines of 94 bovines conveyed to the main slaughterhouse of Ouagadougou from the central and northern part of Burkina Faso allowed the identification of nine different worm species: Cooperia punctata, Cooperia pectinata, Haemonchus contortus, Trichostrongylus colubriformis, Bunostomum phlebotomum, Moniezia expensa, Avitellina sp., Oesophagostomum radiatum, and Trichuris sp. By far, Cooperia sp. was the most prevalent (89.4/), followed...

  3. Inherited Retinal Degenerative Disease Registry

    Science.gov (United States)

    2016-03-21

    Eye Diseases Hereditary; Retinal Disease; Achromatopsia; Bardet-Biedl Syndrome; Bassen-Kornzweig Syndrome; Batten Disease; Best Disease; Choroidal Dystrophy; Choroideremia; Cone Dystrophy; Cone-Rod Dystrophy; Congenital Stationary Night Blindness; Enhanced S-Cone Syndrome; Fundus Albipunctatus; Goldmann-Favre Syndrome; Gyrate Atrophy; Juvenile Macular Degeneration; Kearns-Sayre Syndrome; Leber Congenital Amaurosis; Refsum Syndrome; Retinitis Pigmentosa; Retinitis Punctata Albescens; Retinoschisis; Rod-Cone Dystrophy; Rod Dystrophy; Rod Monochromacy; Stargardt Disease; Usher Syndrome

  4. Crataegus ×ninae-celottiae and C. ×cogswellii (Rosaceae, Maleae), two spontaneously formed intersectional nothospecies

    OpenAIRE

    Knud Ib Christensen; Mehdi Zarrei; Maria Kuzmina; Nadia Talent; Charlotte Lin; Timothy Dickinson

    2014-01-01

    Crataegus monogyna Jacq. is naturalized in North America, where it has hybridized with native diploid hawthorns at least twice. We provide names for the two nothospecies (as well as for the corresponding nothosections and nothoseries), referring to existing documentation in the literature for nothosp. nov. Crataegus ×ninae-celottiae K.I. Chr. & T.A. Dickinson (C. monogyna × C. punctata Jacq.). New data are provided to further document nothosp. nov. Crataegus ×cogswellii ...

  5. Child Neurology: Zellweger syndrome

    OpenAIRE

    Lee, Paul R.; Raymond, Gerald V.

    2013-01-01

    Zellweger syndrome (ZS) is a severe manifestation of disease within the spectrum of peroxisome biogenesis disorders that includes neonatal adrenoleukodystrophy, infantile Refsum disease, and rhizomelic chondroplasia punctata. Patients with ZS present in the neonatal period with a characteristic phenotype of distinctive facial stigmata, pronounced hypotonia, poor feeding, hepatic dysfunction, and often seizures and boney abnormalities. In patients with ZS, a mutation in one of the PEX genes co...

  6. Environmental factors influencing the distribution of three species within the genus Persephona Leach, 1817 (Crustacea, Decapoda, Leucosiidae in two regions on the northern coast of São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    Rafaela T Pereira

    2014-05-01

    Full Text Available Patterns of spatio-temporal distribution of Brachyura are determined by the interaction among life history traits, inter and intraspecific relationships, as well as by the variation of abiotic factors. This study aimed to characterize patterns of spatio-temporal distribution of Persephona lichtensteinii, Persephona mediterranea and Persephona punctata in two regions of the northern coast of São Paulo State, southeastern region of Brazil. Collections were done monthly from July 2001 to June 2003 in Caraguatatuba and Ubatuba, using a shrimp fishery boat equipped with double-rig nets. The patterns of species distribution were tested by means of redundancy analysis (RDA and generalized linear mixed models (GLMM in relation to the recorded environmental factors (BT: bottom temperature, BS: bottom salinity, OM: organic matter and granulometry (Phi. The most influent environmental factor over the species distribution was the Phi, and the ascendant order of influence was P. lichtensteinii, P. punctata and P. mediterranea. The greater abundance of P. mediterranea showed a conservative pattern of distribution for the genus in the sampled region. The greater occurrence of P. punctata and P. lichtensteinii, in distinct transects than those occupied by P. mediterranea, seems to be a strategy to avoid competition among congeneric species, which is related to the substratum specificity.

  7. Natural variation in four human collagen genes across an ethnically diverse population

    OpenAIRE

    Chan, Ting-Fung; Poon, Annie; Basu, Analabha; Addleman, Nick R.; Chen, Justin; Phong, Angie; Byers, Peter H; Klein, Teri E.; Kwok, Pui-Yan

    2008-01-01

    Collagens are members of one of the most important families of structural proteins in higher organisms. There are 28 types of collagens encoded by 43 genes in humans that fall into several different functional protein classes. Mutations in the major fibrillar collagen genes lead to osteogenesis imperfecta (COL1A1 and COL1A2 encoding the chains of Type I collagen), chondrodysplasias (COL2A1 encoding the chains of Type II collagen), and vascular Ehlers-Danlos syndrome (COL3A1 encoding the chain...

  8. Lethal osteochondrodysplasia and de novo autosomal translocation involving the long arm of chromosome 4

    Energy Technology Data Exchange (ETDEWEB)

    Fryns, J.P.; Legius, E.; Van den Berghe, H.; Moerman, P. [Center for Human Genetics, Leuven (Belgium); Vandenberghe, K. [Univ. of Leuven (Belgium); Maroteaux, P. [Hopital des Enfants Malades, Paris (France)

    1994-06-01

    Recently, we diagnosed a de noco t(4q;11q) with apparent breakpoints at 4q23 and 11q13 [karyotype: 46,XX,t(4;11)(q23;q13)] in a first trimester female fetus with a lethal chondrodysplasia. After the observation reported by Urioste et al. (1994) the presena finding of a de novo autosomal translocation with one breakpoint in the proximal part of 4q in another fetus with lethal osteochondrodysplasia may be a further indication toward the possible location of a gene cluster in this autosomal region. 1 ref.

  9. Achondroplasia and periodontal disease

    OpenAIRE

    Chawla, Kirti; Lamba, Arundeep Kaur; Faraz, Farrukh; Tandon, Shruti

    2012-01-01

    Achondroplasia is a non-lethal form of chondrodysplasia. It is a disturbance of endochondral bone formation which results in characteristic dwarfism. It is transmitted as an autosomal dominant trait, with complete penetrance. De novo mutations cause up to 90% of cases. The mutation rate is estimated to be 0.000014 per gamete per generation. It is a rare disorder with a prevalence of 1:10,000 to 1:50,000 births worldwide. A young female patient suffering from achondroplasia reported with oral ...

  10. Approach to Investigating Congenital Skeletal Abnormalities in Livestock.

    Science.gov (United States)

    Dittmer, K E; Thompson, K G

    2015-09-01

    Congenital skeletal abnormalities may be genetic, teratogenic, or nutritional in origin; distinguishing among these different causes is essential in the management of the disease but may be challenging. In some cases, teratogenic or nutritional causes of skeletal abnormalities may appear very similar to genetic causes. For example, chondrodysplasia associated with intrauterine zinc or manganese deficiency and mild forms of hereditary chondrodysplasia have very similar clinical features and histologic lesions. Therefore, historical data are essential in any attempt to distinguish genetic and acquired causes of skeletal lesions; as many animals as possible should be examined; and samples should be collected for future analysis, such as genetic testing. Acquired causes of defects often show substantial variation in presentation and may improve with time, while genetic causes frequently have a consistent presentation. If a disease is determined to be of genetic origin, a number of approaches may be used to detect mutations, each with advantages and disadvantages. These approaches include sequencing candidate genes, single-nucleotide polymorphism array with genomewide association studies, and exome or whole genome sequencing. Despite advances in technology and increased cost-effectiveness of these techniques, a good clinical history and description of the pathology and a reliable diagnosis are still key components of any investigation. PMID:25910781

  11. Comparative analysis of A, B,C and D genomes in the genus Oryza with C0t-1 DNA of C genome

    Institute of Scientific and Technical Information of China (English)

    LAN Weizhen; QIN Rui; LI Gang; HE Guangcun

    2006-01-01

    Fluorescence in situ hybridization (FISH)was applied to somatic chromosomes preparations of Oryza officinalis Wall. (CC), O. sativa L. (AA)×O. officinalis F1 hybrid (AC), backcross progenies BC1 (AAC and ACC), O. latifolia Desv. (CCDD), O. alta Swallen (CCDD) and O. punctata Kotschy (BBCC)with a labelled probe of Cot-1 DNA from O. officinalis.In O. officinalis, the homologous chromosomes showed similar signal bands probed by C0t-1 DNA and karyotype analysis was conducted based on the band patterns. Using no blocking DNA, the probe identified the chromosomes of C genome clearly, but detected few signals on chromosomes of A genome in the F1 hybrid and two backcross progenies of BC1.It is obvious that the highly and moderately repetitive DNA sequences were considerably different between C and A genomes. The chromosomes of C genome were also discriminated from the chromosomes of D-and B-genome in the tetraploid species O. latifolia, O.alta and O. punctata by C0t-1 DNA-FISH. Comparison of the fluorescence intensity on the chromosomes of B, C and D genomes in O. latifolia, O. alta,and O. punctata indicated that the differentiations between C and D genomes are less than that between C and B genomes. The relationship between C and D genomes in O. alta is closer than that of C and D genomes in O. latifolia. This would be one of the causes for the fact that both the genomes are of the same karyotype (CCDD) but belong to different species. The above results showed that the C0t-1 DNA had a high specificity of genome and species. In this paper, the origin of allotetraploid in genus Oryza is also discussed.

  12. Abilities of some higher plants to hydrolyze the acetates of phenols and aromatic-aliphatic alcohols

    Directory of Open Access Journals (Sweden)

    Agnieszka Mironowicz

    2014-02-01

    Full Text Available In the biotransformations carried out under the same conditions, the whole intact plants of Spirodela punctata, Nephrolepis exaltata, Cyrtomium falcatum, Nephrolepis cordifolia and the suspension cultures of Helianthus tuberosus, Daucus carota and Petunia hybrida hydrolyze (partially or totally the ester bonds of the acetates of phenols and aromatic-aliphatic alcohols and also the menthyl acetate. Nevertheless, the methyl esters of aromatic acids, structurally similar to the former substrates, do not undergo hydrolysis. At the same time, the viability of first four plants was observed for different levels of acetate concentration. The method of continuous preparative hydrolysis of the same acetates was worked out in Cyrtomium falcatum culture.

  13. A cytochrome P450 terpenoid hydroxylase linked to the suppression of insect juvenile hormone synthesis

    OpenAIRE

    Sutherland, T. D.; Unnithan, G C; Andersen, J F; Evans, P H; Murataliev, M. B.; Szabo, L. Z.; Mash, E. A.; Bowers, W. S.; Feyereisen, R

    1998-01-01

    A cDNA encoding a cytochrome P450 enzyme was isolated from a cDNA library of the corpora allata (CA) from reproductively active Diploptera punctata cockroaches. This P450 from the endocrine glands that produce the insect juvenile hormone (JH) is most closely related to P450 proteins of family 4 and was named CYP4C7. The CYP4C7 gene is expressed selectively in the CA; its message could not be detected in the fat body, corpora cardiaca, or brain, but trace levels of expression were found in the...

  14. Dicty_cDB: Contig-U15362-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available a punctata genomic clo... 36 1.2 2 ( EY852787 ) CG32-C1-003-030-E05-CT.F Mexican lime leaf, green... 38 1.3 ...can lime leaf, green... 38 4.8 2 ( EY855107 ) CG32-C1-003-014-F02-CT.F Mexican lime...003... 34 4.5 6 ( CU104698 ) Zebrafish DNA sequence from clone CH211-266L9 in ... 36 4.8 4 ( EY851729 ) CG32-C1-003-008-F01-CT.F Mexi

  15. fRNAdb Summary: FR303191 [

    Lifescience Database Archive (English)

    Full Text Available FR303191 AF314834,AF314837,AF314838,AF314840,AF314844,AF314847,AF314848,AF314849,AF314851,AF3148 ... 18840,AY918841,BA000029,DQ091229,DQ091230,X57967 Gro up II intro n gro up_II_intro n Pistia stratio tes,O ryz ... a australiensis,O ryza brachyantha,O ryza o fficinalis,O ryza punctata,T ... riticum aestivum,Zea mays,Ario psis peltata,Xantho so ma sagittifo lium,Cypripedium a ... caule,Thunia alba,Calanthe vestita,Go upia glabra,Agave attenuata,O ryza sativa (indica cu ...

  16. Planktonic cnidarians in a cold-core ring in the Gulf of Mexico

    OpenAIRE

    E. Suárez MORALES; R. Gasca; L. Segura PUERTAS; D. C. Biggs

    2002-01-01

    The species composition and abundance of medusae and siphonophores collected in March 1993 across a Gulf of Mexico cold-core ring (CCR) were analysed. Samples were collected in day and night time by oblique tows (100 m to surface) with a plankton net. Medusae were represented by 12 species, which by combined numbers were almost equally abundant within and outside the CCR. However, the two species that together comprised 66% of all medusae inside the CCR (Nausithoë punctata and Liriope tetraph...

  17. Screening antifungal activities of selected medicinal plants.

    Science.gov (United States)

    Quiroga, E N; Sampietro, A R; Vattuone, M A

    2001-01-01

    Plants synthesise a vast array of secondary metabolites that are gaining importance for their biotechnological applications. The antifungal activity of the ethanolic extracts of ten Argentinean plants used in native medicine is reported. Antifungal assays included radial growth inhibition, disk and well diffusion assays and growth inhibition by broth dilution tests. The chosen test fungi were yeasts, microfungi and wood-rot causing Basidiomycetes. Extracts of Larrea divaricata, Zuccagnia punctata and Larrea cuneifolia displayed remarkable activity in the assays against the majority of the test fungi. In addition to the former plants, Prosopanche americana also inhibited yeast growth. PMID:11137353

  18. Evaluación ambiental del río San Juan de Santiago de Cuba por exposición bioacumulativa a metales pesados Environmental evaluation of San Juan river in Santiago de Cuba due to bioaccumulative exposure to heavy metals

    OpenAIRE

    George Argota Pérez; Humberto Argota Coello

    2012-01-01

    Se evaluó la salud ambiental del río San Juan de Santiago de Cuba, expuesto a metales pesados, mediante la utilización de la especie biorreguladora Gambusia punctata. Se analizaron 271 individuos adultos clasificados por sexo, para lo cual se escogieron los intervalos de clases de edades 2,1-3,0 y 3,1-4,0 cm, según la longitud total, respectivamente. La mezcla ácida aplicada estuvo compuesta por ácido perclórico - ácido sulfúrico; (7:1) ácido nítrico para determinar los niveles bioacumulados ...

  19. Spatial segregation of freshwater fish in an intermittent Cuban stream.

    Directory of Open Access Journals (Sweden)

    José Luis Ponce de León

    2013-09-01

    Full Text Available The spatial segregation of fish species is related to the use of available resources in every river or lake segment. In this work the spatial distribution of four endemic and one introduced freshwater fish species from the Govea stream, in Havana Province, Cuba was studied between june and november of 2005. Both, the effect of sun incidence and the segregation in the water column were analyzed. Gambusia punctata Poey, 1854 feeds on invertebrates, mainly on the surface zone, while herbivores like Girardinus metallicus Poey, 1854 are found in the intermediate zone more frequently. The detritivores like Limia vittata Guichenot, 1853 and Plecostomus commersoni Valenciennes, 1836, and the omnivorous cichlid Nandopsis tetracanthus (Cuvier y Valenciennes, 1831, were found mostly at the bottom. Differences in the mean number of individuals were found for most species in same-depth transects except for N. tetracanthus. In zones exposed to direct sunlight G. metallicus, L. vittata and P. commersoni were found more abundant, whereas G. punctata was mainly noticed in shade areas.

  20. New mammalian records in the Parque Nacional Cerros de Amotape, northwestern Peru

    Directory of Open Access Journals (Sweden)

    Cindy M. Hurtado

    2015-04-01

    Full Text Available The Pacific Tropical Rainforest and Equatorial Dry Forest are found only in southern Ecuador and northern Peru, and are among the most poorly known ecosystems of South America. Even though these forests are protected in Parque Nacional Cerros de Amotape (PNCA, they are threatened by fragmentation because of farming and agriculture. The aim of this study was to determine the medium and large mammalian species richness, using transect census, camera trapping, and specimen bone collection. Nine transects were established and 21 camera trap stations were placed along 16 km2 in three localities of PNCA, from August 2012 to April 2013. Total sampling effort was 215 km of transects and 4077 camera-days. We documented 22 species; including 17 with camera trapping, 11 with transect census, and 10 with specimen collection.  Camera traps were the most effective method, and four species (Dasyprocta punctata, Cuniculus paca, Leopardus wiedii and Puma concolor were documented only with this method. This comprised the first Peruvian record for Dasyprocta punctata, and the first record for the western slope of the Peruvian Andes for Cuniculus paca. Also, both specimen collections and sightings confirm the presence of Potos flavus, first record in the western slope of the Peruvian Andes. Panthera onca, Tremarctos ornatus and Saimiri sciureus are considered locally extinct, while several species are in need of further research. We highlight the importance of the high diversity of this rainforests and encourage local authorities to give the area the highest priority in conservation.

  1. The influence of duckweed species diversity on ecophysiological tolerance to copper exposure.

    Science.gov (United States)

    Zhao, Zhao; Shi, Huijuan; Duan, Dongzhu; Li, Hongmei; Lei, Tingwen; Wang, Maolin; Zhao, Hai; Zhao, Yun

    2015-07-01

    In excess, copper is toxic to plants. In the plants, Landoltia punctata and Lemna minor grown in mixed and monoculture, the effects of exposure to varying concentrations of copper (0.01, 0.1, 0.5 and 1mgL(-1) Cu) for seven days were assessed by measuring changes in the chlorophyll, protein and malondialdehyde (MDA) content, catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) activity. According to results, Cu levels in plants increased with increasing Cu concentration. The level of photosynthetic pigments and crude proteins decreased only upon exposure to high Cu concentrations. However, the starch and malondialdehyde (MDA) content increased. These results suggested a stress alleviation that was possibly the result of antioxidants such as CAT and SOD, the activities of which increased with increasing Cu levels. APX activity increased in L. punctata, but decreased in L. minor, under monoculture or mixed culture conditions. In addition, the duckweed in mixed culture exhibited increased antioxidant enzyme activities which provide increased resistance to copper in moderate copper concentrations. As the copper concentration increased, the duckweed in the mixed culture limited the uptake of copper to avoid toxicity. PMID:25938979

  2. First molecular detection of Theileria ovis in Rhipicephalus sanguineus tick in Iran

    Institute of Scientific and Technical Information of China (English)

    Telmadarraiy Zakkyeh; Oshaghi Mohammad Ali; Hosseini Vasoukolaei Nasibeh; Yaghoobi Ershadi Mohammad Reza; Babamahmoudi Farhang; Mohtarami Fatemeh

    2012-01-01

    Objective:To determine tick infestation of domestic ruminants and their infection to ovine theileriosis in northern Iran. Methods:About 425 domestic ruminants in Ghaemshahr city in northern Iran were inspected for tick infestations. Twenty tick specimens (13 females and 7 males) of Rhipicephalus sanguineus (R. sanguineus), the most common tick in the study area, were tested by PCR amplification against 18s rRNA genome of Theileria spp using specie specific primers and then the PCR products were sequenced for species identification by comparison with data base available in GenBank. Results:About 323 ticks were collected from 102 animals (88 sheep, 12 goats and 2 cattle). The prevalence of ticks infesting animals was R. sanguineus (82.35%), Rhipicephalus bursa (R. bursa) (0.3%), Ixodes ricinus (I. ricinus) (15.2%), Boophilus annulatus (B. annulatus) (1.2%), Haemaphysalis punctata (H. punctata) (0.3%) and Haemaphysalis numidiana (H. numidiana) (0.6%). Eleven (55%) tick specimens were PCR positive against genome of Theileria ovis (T. ovis). Sequence analysis of the PCR products confirmed presence of T. ovis in one R. sanguinus. Conclusions:This is the first report of tick infection to T. ovis in Iran. Due to dominant prevalence of R. sanguineus as well as its infection to T. ovis, it is postulated this tick is the main vector of ovine theileriosis in northern Iran.

  3. Identification of Chromosomes from Multiple Rice Genomes Using a Universal Molecular Cytogenetic Marker System

    Institute of Scientific and Technical Information of China (English)

    Xiaomin Tang; Weidong Bao; Wenli Zhang; Zhukuan Cheng

    2007-01-01

    To develop reliable techniques for chromosome identification is critical for cytogenetic research, especially for genomes with a large number and smaller-sized chromosomes. An efficient approach using bacterial artificial chromosome (BAG) clones as molecular cytological markers has been developed for many organisms. Herein, we present a set of chromosomal arm-specific molecular cytological markers derived from the gene-enriched regions of the sequenced rice genome. All these markers are able to generate very strong signals on the pachytene chromosomes of Oryza satlva L. (AA genome) when used as fluorescence in situ hybridization (FISH) probes. We further probed those markers to the pachytene chromosomes of O. punctata (BB genome) and O. officinalis (CC genome) and also got very strong signals on the relevant pachytene chromosomes. The signal position of each marker on the related chromosomes from the three different rice genomes was pretty much stable, which enabled us to identify different chromosomes among various rice genomes. We also constructed the karyotype for both O. punctata and O. officinalis with the BB and CC genomes, respectively, by analysis of 10 pachytene cells anchored by these chromosomal arm-specific markers.

  4. Notas e descrições em Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. Novos táxons, nova sinonímia e novos registros

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2007-01-01

    Full Text Available Notas e descrições em Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. Novos táxons e novos registros. Descrição de espécies novas: Zikanita egregia sp. nov. (Bolívia, Santa Cruz; Dufauxia thomasi sp. nov. (Bolívia, Santa Cruz; Irundisaua punctata sp. nov. (Colômbia, Cauca; Cotyzineus gen. nov., espécie-tipo C. bruchi (Melzer, 1931 comb. nov. Novos registros: Zikanita argenteofasciata (Tippmann, 1960 é assinalada para o Brasil (Maranhão e Cotyzineus bruchi para a Bolívia (Santa Cruz. Novo sinônimo: Ateralphus diringsi Martins & Monné, 1993 = A. javariensis Lane, 1965.Notes and descriptions on Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. New taxa and new records. New taxa described: Zikanita egregia, sp. nov. from Bolivia (Santa Cruz; Dufauxia thomasi sp. nov. from Bolivia (Santa Cruz; Irundisaua punctata sp. nov. from Colombia (Cauca; Cotyzineus gen. nov. type species C. bruchi (Melzer, 1931 comb. nov. New records: Zikanita argenteofasciata (Tippmann, 1960 for Brazil (Maranhão; Cotyzineus bruchi for Bolivia (Santa Cruz. New synomym: Ateralphus diringsi Martins & Monné, 1993 = A. javariensis Lane, 1965.

  5. Cubozoa e Scyphozoa (Cnidaria: Medusozoa de águas costeiras do Brasil Cubozoa and Scyphozoa (Cnidaria: Medusozoa from Brazilian coastal waters

    Directory of Open Access Journals (Sweden)

    André C. Morandini

    2005-09-01

    Full Text Available As espécies de Cubozoa e Scyphozoa costeiras que ocorrem no Brasil são descritas, com base em espécimes de coleções de museus e exemplares recém-coletados. Chaves de identificação e um glossário também são apresentados. As espécies descritas são: Aurelia sp.; Cassiopea xamachana Bigelow, 1892; Chiropsalmus quadrumanus (Müller, 1859; Chrysaora lactea Eschscholtz, 1829; Drymonema dalmatinum Haeckel, 1880; Linuche unguiculata (Swartz, 1788; Lychnorhiza lucerna Haeckel, 1880; Nausithoe aurea Silveira & Morandini, 1997; Phyllorhiza punctata von Lendenfeld, 1884; Stomolophus meleagris Agassiz, 1862; Tamoya haplonema Müller, 1859 e Tripedalia cystophora Conant, 1897.Coastal species of Cubozoa and Scyphozoa occurring in Brazil are described, based on museum specimens and recently collected ones. Identification keys and a glossary are also presented. The listed species are: Aurelia sp.; Cassiopea xamachana Bigelow, 1892; Chiropsalmus quadrumanus (Müller, 1859; Chrysaora lactea Eschscholtz, 1829; Drymonema dalmatinum Haeckel, 1880; Linuche unguiculata (Swartz, 1788; Lychnorhiza lucerna Haeckel, 1880; Nausithoe aurea Silveira & Morandini, 1997; Phyllorhiza punctata von Lendenfeld, 1884; Stomolophus meleagris Agassiz, 1862; Tamoya haplonema Müller, 1859; and Tripedalia cystophora Conant, 1897.

  6. Ellis-van Creveld syndrome in an Indian child: a case report

    Energy Technology Data Exchange (ETDEWEB)

    Veena, K.M.; Jagadishchandra, H.; Rao, Prasanna Kumar; Chatra, Laxmikanth [Yenepoya Dental College, Yenepoya University, Mangalore (India)

    2011-12-15

    Ellis-van Creveld syndrome is a rare congenital genetic disorder having autosomal recessive inheritance. It is a syndrome affecting the Amish population of Pennsylvania in USA with prevalence rate of 1/5,000 live at birth. In non-Amish population, the birth prevalence is 7/1,000,000. The syndrome is characterized by bilateral postaxial polydactyly of the hands, chondrodysplasia of long bones resulting in acromesomelic dwarfism, ectodermal dysplasia affecting nails as well as teeth and congenital heart malformation. There were very rare reports of this syndrome in dentistry. The present case focuses on the striking and constant oral findings of these patients, which are the main diagnostic features of this syndrome. Since the oral manifestations affect the esthetic, speech, and jaw growth of the child, the dentists have an important role to play in proper management of such case.

  7. Ellis-van Creveld syndrome in an Indian child: a case report

    International Nuclear Information System (INIS)

    Ellis-van Creveld syndrome is a rare congenital genetic disorder having autosomal recessive inheritance. It is a syndrome affecting the Amish population of Pennsylvania in USA with prevalence rate of 1/5,000 live at birth. In non-Amish population, the birth prevalence is 7/1,000,000. The syndrome is characterized by bilateral postaxial polydactyly of the hands, chondrodysplasia of long bones resulting in acromesomelic dwarfism, ectodermal dysplasia affecting nails as well as teeth and congenital heart malformation. There were very rare reports of this syndrome in dentistry. The present case focuses on the striking and constant oral findings of these patients, which are the main diagnostic features of this syndrome. Since the oral manifestations affect the esthetic, speech, and jaw growth of the child, the dentists have an important role to play in proper management of such case.

  8. Human Genetic Disorders and Knockout Mice Deficient in Glycosaminoglycan

    Directory of Open Access Journals (Sweden)

    Shuji Mizumoto

    2014-01-01

    Full Text Available Glycosaminoglycans (GAGs are constructed through the stepwise addition of respective monosaccharides by various glycosyltransferases and maturated by epimerases and sulfotransferases. The structural diversity of GAG polysaccharides, including their sulfation patterns and sequential arrangements, is essential for a wide range of biological activities such as cell signaling, cell proliferation, tissue morphogenesis, and interactions with various growth factors. Studies using knockout mice of enzymes responsible for the biosynthesis of the GAG side chains of proteoglycans have revealed their physiological functions. Furthermore, mutations in the human genes encoding glycosyltransferases, sulfotransferases, and related enzymes responsible for the biosynthesis of GAGs cause a number of genetic disorders including chondrodysplasia, spondyloepiphyseal dysplasia, and Ehlers-Danlos syndromes. This review focused on the increasing number of glycobiological studies on knockout mice and genetic diseases caused by disturbances in the biosynthetic enzymes for GAGs.

  9. Paleopathological Study of Dwarfism-Related Skeletal Dysplasia in a Late Joseon Dynasty (South Korean Population.

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    Eun Jin Woo

    Full Text Available Skeletal dysplasias related to genetic etiologies have rarely been reported for past populations. This report presents the skeletal characteristics of an individual with dwarfism-related skeletal dysplasia from South Korea. To assess abnormal deformities, morphological features, metric data, and computed tomography scans are analyzed. Differential diagnoses include achondroplasia or hypochondroplasia, chondrodysplasia, multiple epiphyseal dysplasia, thalassemia-related hemolytic anemia, and lysosomal storage disease. The diffused deformities in the upper-limb bones and several coarsened features of the craniofacial bones indicate the most likely diagnosis to have been a certain type of lysosomal storage disease. The skeletal remains of EP-III-4-No.107 from the Eunpyeong site, although incomplete and fragmented, provide important clues to the paleopathological diagnosis of skeletal dysplasias.

  10. New mutations in DYNC2H1 and WDR60 genes revealed by whole-exome sequencing in two unrelated Sardinian families with Jeune asphyxiating thoracic dystrophy.

    Science.gov (United States)

    Cossu, Carla; Incani, Federica; Serra, Maria Luisa; Coiana, Alessandra; Crisponi, Giangiorgio; Boccone, Loredana; Rosatelli, Maria Cristina

    2016-04-01

    Jeune asphyxiating thoracic dystrophy (JATD; Jeune syndrome, MIM 208500) is a rare autosomal recessive chondrodysplasia, phenotypically overlapping with short-rib polydactyly syndromes (SRPS). JATD typical hallmarks include skeletal abnormalities such as narrow chest, shortened ribs, limbs shortened bones, extra fingers and toes (polydactyly), as well as extraskeletal manifestations (renal, liver and retinal disease). To date, disease-causing mutations have been found in several genes, highlighting a marked genetic heterogeneity that prevents a molecular diagnosis of the disease in most families. Here, we report the results of whole-exome sequencing (WES) carried out in four JATD cases, belonging to three unrelated families of Sardinian origin. The exome analysis allowed to identify mutations not previously reported in the DYNC2H1 (MIM 603297) and WDR60 (MIM 615462) genes, both codifying for ciliary intraflagellar transport components whose mutations are known to cause Jeune syndrome. PMID:26874042

  11. Paleopathological Study of Dwarfism-Related Skeletal Dysplasia in a Late Joseon Dynasty (South Korean) Population

    Science.gov (United States)

    Woo, Eun Jin; Lee, Won-Joon; Hu, Kyung-Seok; Hwang, Jae Joon

    2015-01-01

    Skeletal dysplasias related to genetic etiologies have rarely been reported for past populations. This report presents the skeletal characteristics of an individual with dwarfism-related skeletal dysplasia from South Korea. To assess abnormal deformities, morphological features, metric data, and computed tomography scans are analyzed. Differential diagnoses include achondroplasia or hypochondroplasia, chondrodysplasia, multiple epiphyseal dysplasia, thalassemia-related hemolytic anemia, and lysosomal storage disease. The diffused deformities in the upper-limb bones and several coarsened features of the craniofacial bones indicate the most likely diagnosis to have been a certain type of lysosomal storage disease. The skeletal remains of EP-III-4-No.107 from the Eunpyeong site, although incomplete and fragmented, provide important clues to the paleopathological diagnosis of skeletal dysplasias. PMID:26488291

  12. A study of four medicinal plant complexes of Mexico and adjacent United States.

    Science.gov (United States)

    Linares, E; Bye, R A

    1987-01-01

    A survey of medicinal herbs in markets of central and northern Mexico and southwestern United States revealed the existence of plant complexes of different species sharing common names, morphological and aromatic characteristics, and uses. Four complexes (with the "label" species listed first) discussed include: "cachani" with Roldana sessilifolia, Iostephane madrensis, Liatris punctata, Psacalium sp., and Potentilla sp.; "chuchupate" with Ligusticum porteri and Myroxylon balsamum; "hierba anís" with Tagetes lucida, T. filifolia, T. micrantha, Artemisia dracunculus, Pimpinella anisum, and Illicium verum; and "matarique" with Psacalium decompositum, P. peltatum, P. sinuatum, P. sp., and Acourtia thurberi. The distribution analysis of utilization and natural occurrence of plants in each complex indicated the presence of a dominant or "label" plant whose use extended beyond its natural range and which had substitutes derived from local plants that were not registered far beyond their respective natural ranges. PMID:3613608

  13. FIRST RECORD OF TWO SPECIES OF FELLODISTOMID TREMATODES IN SOUTH CHINA SEA%壮穴科吸虫两种地理新记录

    Institute of Scientific and Technical Information of China (English)

    刘升发; 杨廷宝

    2003-01-01

    报道寄生于斑点鸡笼鲳 Drepane punctata(Linnaeus)消化道的阿拉伯类腹盘吸虫 Paradiscogaster arabi(Hafeezullah & Siddiqi, 1970) Cribb, Anderson & Bray, 1999 和寄生于金钱鱼 Scatophagus argus (Linnaeus)消化道的福氏类腹盘吸虫 Paradiscogaster farooqii Hafeezullah & Siddiqi, 1970在中国属新发现,并将中国的标本同国外的标本进行比较.首次观察到Paradiscogaster arabi的排泄囊为"V"型;而Paradiscogaster farooqii的排泄囊形状则与Nahhas, Sey and Nishimoto (1998) 和Cribb ,Anderson & Bray (1999)所报道的不同.首次绘制出这二种吸虫卵巢复合体的结构.

  14. Efficacy of an albendazole feed formulation against bovine gastrointestinal nematodes including arrested larvae of Ostertagia ostertagi.

    Science.gov (United States)

    Courtney, C H; Greiner, E C; Whitten, R D

    1986-01-01

    The efficacy of an albendazole feed premix formulation was compared with that of an albendazole drench suspension for control of gastrointestinal nematodes in 31 beef cattle. The premix (11 cattle) and drench suspension (9 cattle) were found to have similar efficacies at a dosage of 7.5 mg/kg of body weight. When compared with controls (11 cattle), both formulations caused significant (P less than 0.05) reductions in worm counts with an efficacy of 98% or greater against adult Haemonchus placei, Ostertagia ostertagi, Trichostrongylus axei, Cooperia punctata, and C pectinata. There was no significant effect against arrested 4th-stage larvae of O ostertagi. Adverse effects of albendazole treatment were not observed, and the premix formulation was readily consumed by cattle. PMID:3946888

  15. Essential Oil Composition of Three Globularia Species.

    Science.gov (United States)

    Crkvenčić, Maja; Dudaš, Slavica; Jerković, Igor; Marijanović, Zvonimir; Poljuha, Danijela; Pilepić, Kroata Hazler

    2016-02-01

    The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Globularia cordifolia L., G. meridionalis (Podp.) O.Schwarz, and G. punctata Lapeyr. was characterized by GC-FID and GC/MS analyses. Among the 33 identified compounds, the most abundant present in all investigated samples were oct-1-en-3-ol (2.9-47.0%), 6-(1,5-dimethylhex-4-enyl)-3-methylcyclohex-2-enone (8.2-40.9%), and fukinanolid (7.4-31.6%). Multivariate statistical analyses (PCA and HCA) of the hitherto studied Globularia volatile compounds confirmed to some extent the assumed phylogenetic relationships of the Globularia species studied, including the close relationship between the morphologically similar species G. cordifolia and G. meridionalis, but also evidenced several discrepancies in the current classification of Globularia species. PMID:26880434

  16. ANATOMY AND MORPHOLOGY OF VEGETATIVE STRUCTURES OF EIGHT SPECIES OF POACEAE IN CIENEGUILLA, LIMA, PERU

    Directory of Open Access Journals (Sweden)

    Davila, J.

    2008-01-01

    Full Text Available Anatomy and morphology of vegetative structures of eight species of Poaceae collected in the zone of Cieneguilla, in Lurin riverbank (12° 04´ 23.47” S; 76° 45´54.38” W, Lima, Peru were studied. The species collected and analyzed were distributed in 4-sub families and 6 tribes, which were: Phragmites comunis, Digitaria sp., Cenchrus sp., Eriochloa punctata, Gynerium sp., Cynodon dactilon, Leptochloa sp. and Polypogon sp. For the study of morphology and anatomy was used methodology proposed by Johansen. For the morphology of the species collected was obtained in general, that present similarity to each other, but with variable sizes. Greater contrast was obtained at anatomical level where differences as far as the disposition of the vascular vessels were appraised in the stem and the presence of equifacial or bifacial anatomy in the leaves.

  17. Chondroectodermal dysplasia (Ellis van Creveld syndrome: A report of three cases with review of literature

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    Kurian K

    2007-01-01

    Full Text Available Chondroectodermal dysplasia is a rare mesenchymal - ectodermal dysplasia first described in 1940 by Richard W.B. Ellis and Simon van Creveld now known as Ellis van Creveld syndrome. It is also known as Mesvectodermal dysplasia. Majority of cases were characteristically seen in one particular inbred population from the Amish community of Lancaster County, Pennsylvania, U.S.A. The syndrome manifests with several skeletal anomalies, oral mucosal and dental anomalies, congenital cardiac defects and nail dysplasia. Ellis van Creveld syndrome may be differentiated from other chondrodystrophies like achondroplasia, chondroplasia punctata, asphyxiating thorasic dystrophy and Morquio′s syndrome. The presence of oral mucosal and dental alterations like notching of the lower alveolar process, fusion of the upper lip with gingival mucosal margin, occasional presence of neonatal teeth, oligodontia and conical shape of anterior teeth will confirm the diagnosis of Ellis van Creveld syndrome and hence its importance to dentists.

  18. Invertebrates associated with ipomea aquatica in ogbe creek, logos, nigeria

    International Nuclear Information System (INIS)

    The association of invertebrates in Ogbe creek with Ipomea aquatica was investigated within the period from 7th September to 30th November, 2001, 167 invertebrates comprising of 19 species were harvested from 73 weeds. Corixa punctata (22.16%) was the most abundant invertebrate on Ipomea aquatica while Gyrinus notator larvae (0.60%) were the least abundant. The roots sheltered the highest number of invertebrates (113), comprising of 12 species recording a species diversity of 5.36 while the stem sheltered the lowest number of invertebrates (10) comprising of 3 species with a species diversity of 2.00. The ability of Ipomea aquaTica to harbour invertebrates was influenced by the morphological form of the plant. The root was the preferred site for the invertebrates because it was a suitable substrate for clinging and nutrient supply. (author)

  19. Crataegus ×ninae-celottiae and C. ×cogswellii (Rosaceae, Maleae, two spontaneously formed intersectional nothospecies

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    Knud Ib Christensen

    2014-04-01

    Full Text Available Crataegus monogyna Jacq. is naturalized in North America, where it has hybridized with native diploid hawthorns at least twice. We provide names for the two nothospecies (as well as for the corresponding nothosections and nothoseries, referring to existing documentation in the literature for nothosp. nov. Crataegus ×ninae-celottiae K.I. Chr. & T.A. Dickinson (C. monogyna × C. punctata Jacq.. New data are provided to further document nothosp. nov. Crataegus ×cogswellii K.I. Chr. & T.A. Dickinson (C. monogyna × C. suksdorfii (Sarg. Kruschke. In both cases, the striking differences in leaf shape between most New World hawthorns and Old World section Crataegus, and the intermediacy of the hybrids, account for the relative ease with which these hybrids can be recognized. Finally, new sequence data from ITS2 and chloroplast DNA barcoding loci confirm the genetic relationships between the two nothospecies and their respective parents.

  20. Crataegus ×ninae-celottiae and C. ×cogswellii (Rosaceae, Maleae), two spontaneously formed intersectional nothospecies.

    Science.gov (United States)

    Christensen, Knud Ib; Zarrei, Mehdi; Kuzmina, Maria; Talent, Nadia; Lin, Charlotte; Dickinson, Timothy A

    2014-01-01

    Crataegus monogyna Jacq. is naturalized in North America, where it has hybridized with native diploid hawthorns at least twice. We provide names for the two nothospecies (as well as for the corresponding nothosections and nothoseries), referring to existing documentation in the literature for nothosp. nov. Crataegus ×ninae-celottiae K.I. Chr. & T.A. Dickinson (C. monogyna × C. punctata Jacq.). New data are provided to further document nothosp. nov. Crataegus ×cogswellii K.I. Chr. & T.A. Dickinson (C. monogyna × C. suksdorfii (Sarg.) Kruschke). In both cases, the striking differences in leaf shape between most New World hawthorns and Old World section Crataegus, and the intermediacy of the hybrids, account for the relative ease with which these hybrids can be recognized. Finally, new sequence data from ITS2 and chloroplast DNA barcoding loci confirm the genetic relationships between the two nothospecies and their respective parents. PMID:24843290

  1. Description of five species of Xanthopimpla Saussure 1892 (Hymenoptera: Ichneumonidae: Pimplinae) from Malaysia

    Science.gov (United States)

    Dass, Angeline David; Ghani, Idris Abd.

    2013-11-01

    Description of five species of Xanthopimpla Saussure, 1829 (Hymenoptera: Ichneumonidae: Pimplinae) from Malaysia was done using specimens deposited in Centre for Insects Systematics, Universiti Kebangsaan Malaysia (CIS, UKM). Type and non-type specimens were loaned from several repositories namely Zoological Museum of Amsterdam Netherlands (ZMAN), Swedish Museum of Natural History (NRM), British Natural History Museum London (BMNH) and Department of Agricultural Malaysia (DOA) for identification and comparison. The specimens were identified to the species level which gives rise to five species namely Xanthopimpla conica Cushman, 1925, Xanthopimpla despinosa leipephelis Townes & Chiu, 1970, Xanthopimpla flavolineata Cameron, 1907, Xanthopimpla punctata (Fabricius, 1781) and Xanthopimpla tricapus impressa Townes & Chiu, 1970. A dichotomous key and descriptions for five Xanthopimpla spesies were provided. Photos and illustrations of carina on propodeum were also included in this paper.

  2. Contact dermatitis from Larrea (creosote bush).

    Science.gov (United States)

    Leonforte, J F

    1986-02-01

    Six men suffering from acute dermatitis had positive patch tests to Larrea (creosote bush). The lesions preferentially involved sun-exposed sites, simulating a photodermatitis, but also were on the legs and scrotum. Our findings were more consistent with contact allergy than with a primary irritant or a phototoxic response. The patch tests were also positive to Zuccagnia punctata. In two cases the exposure to the creosote bush occurred as a result of casual occupations, in two because of household remedies (moist compresses and baths), and in the other two as a result of burning the bush and resorting to household remedies. Attention should be drawn to this contact dermatitis because the creosote bush grows abundantly all over the American continent. PMID:3950120

  3. TREPOSTOME AND CRYPTOSTOME BRYOZOANS FROM THE KONĔPRUSY LIMESTONE (LOWER DEVONIAN, PRAGIAN OF ZLATÝ KŮŇ (CZECH REPUBLIC

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    ANDREJ ERNST

    2008-11-01

    Full Text Available The present paper presents descriptions of 10 bryozoan species from material deposited at the Geological Centrum Göttingen, Germany. The studied material comes from the Lower Devonian rocks (Konĕprusy Limestone, Pragian, exposed in a quarry near Zlatý Kůň in Central Bohemia. Described bryozoans comprise 8 trepostome and 2 cryptostome (rhabdomesine species. One genus and species is new: the cryptostome (rhabdomesine Paracuneatopora striata gen. n., n. sp., and 7 species are new: trepostomes Leioclema elegans n. sp., Atactotoechus divulgatus n. sp., Loxophragma fistulosum n. sp., Leptotrypella punctata n. sp., Microcampylus obscurus n. sp., Dyscritella bohemica n. sp., and the rhabdomesine Orthopora tenuis n. sp. Furthermore, two trepostomes Monotrypa bohemica Prantl, 1933 and Anomalotoechus cf. typicus Duncan, 1939 were identified in this material. The described fauna displays palaeozoogeographic relations to the Lower Devonian (Pragian of Morocco and the Middle Devonian of Michigan (USA. 

  4. Screening of lipid degrading microorganisms for wastewater treatment

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    Sarmurzina, Z. S.

    2013-01-01

    Full Text Available Aims: Fats, oils and greases (FOG are poorly removable materials in wastewater treatment systems. The aim of this work is to find the most suitable strain(s for a biological treatment technology of FOGs polluted wastewaters. Methodology and results: The 142 microorganisms from polluted environment were screened for lipase activity (LA by sequentially using assays on agar-Tween 80, agar-fats, and turbidimetrically measuring the quantity of calcium salts with fatty acids. The isolates G23, G30, and Zb32 showed highest units of LA and were identified by sequence analysis of 16S rRNA genes. Lipid masses were determined gravimetrically after chloroform/ethyl alcohol extraction. In the model solutions with animal fats the strain Pseudomonas aeruginosa G23 reduced mass fractions of mutton fat, beef tallow, and lard by 79±5%, 88±4%, and 80±6% respectively. Under the same conditions Aeromonas punctata G30 reduced: 65±3%, 60±8%, and 75±4%, and P. aeruginosa Zb32 reduced: 47±5%, 52±6% and 73±7%. In the model solutions with FOGs trap specimens as a carbon source from the local cafeteria the strains P. aeruginosa G23, A. punctata G30, and P. aeruginosa Zb32 reduced a lipid mass fraction by 61.5±7%, 45.2±5%, and 37.5±3% respectively.Conclusion, significance and impact of study: The strain P. aeruginosa G23 is the most effective lipid-degrading microorganism and the best candidate to use in biological treatment technology of FOGs polluted wastewater in Kazakhstan.

  5. New U-Pb zircon ages and the duration and division of Devonian time

    Science.gov (United States)

    Tucker, R.D.; Bradley, D.C.; Ver Straeten, C.A.; Harris, A.G.; Ebert, J.R.; McCutcheon, S.R.

    1998-01-01

    Newly determined U-Pb zircon ages of volcanic ashes closely tied to biostratigraphic zones are used to revise the Devonian time-scale. They are: 1) 417.6 ?? 1.0 Ma for an ash within the conodont zone of Icriodus woschmidti/I. w. hesperius Lochkovian); 2) 408.3 ?? 1.9 Ma for an ash of early Emsian age correlated with the conodont zones of Po. dehiscens--Lower Po. inversus; 3) 391.4 ?? 1.8 Ma for an ash within the Po. c. costatus Zone and probably within the upper half of the zone (Eifelian); and 4) 381.1 ?? 1.3 Ma for an ash within the range of the Frasnian conodont Palmatolepis punctata (Pa. punctata Zone to Upper Pa. hassi Zone). U-Pb zircon ages for two rhyolites bracketing a palyniferous bed of the pusillites-lepidophyta spore zone, are dated at 363.8 ?? 2.2 Ma and 363 ?? 2.2 Ma and 363.4 ?? 1.8 Ma, respectively, suggesting an age of ~363 Ma for a level within the late Famennian Pa. g. expansa Zone. These data, together with other published zircon ages, suggest that the base and top of the Devonian lie close to 418 Ma and 362 Ma, respectively, thus lengthening the period of ~20% over current estimates. We suggest that the duration of the Middle Devonian (Eifelian and Givitian) is rather brief, perhaps no longer than 11.5 Myr (394 Ma-382.5 Ma), and that the Emsian and Famennian are the longest stages in the period with estimated durations of ~15.5 Myr and 14.5 Myr, respectively.

  6. Anti-inflammatory, Antioxidant and Antimicrobial Activity Characterization and Toxicity Studies of Flowers of "Jarilla", a Medicinal Shrub from Argentina.

    Science.gov (United States)

    Moreno, Alejandra; Nuño, Gabriela; Cuello, Soledad; Sayago, Jorge E; Alberto, María Rosa; Zampini, Catiana; Isla, María Inés

    2015-06-01

    Zuccagnia punctata Cav. (Fabaceae) is an Argentine medicinal aromatic shrub (jarilla pispito, puspus, lata and jarilla macho). The chalcones were identified as pigments responsible for the yellow color of the flowers. Hydroethanolic extracts were obtained both from fresh flowers and from flowers dried by lyophilization. The extracts were standardized by their phenolic and flavonoids content. Their fingerprints by HPLC-DAD indicated the presence of two chalcones as major compounds (2',4'-dihydroxychalcone and 2',4'-dihydroxy-3'-methoxychalcone). Both extracts showed the same total phenolic, non-flavonoid phenolic and flavonoid phenolic content and their phenolic profiles were similar. The polyphenolic extracts exhibited antioxidant (free radical scavenging and inhibitory activity on lipoperoxidation) and anti-inflammatory (inhibition of lipoxygenase and cyclooxygenase enzymes) activities. The flower extracts were active against six Candida species with MIC values between 60 and 120 μg GAE x mL(-1) and were also active on methicillin-resistant Staphylococcus aureus (MIC: 250 μg GAE x mL(-1)) and Enterococcus faecalis (MIC: 500 μg GAE x mL(-1)). The extracts were neither toxic (Artemia salina test) nor mutagenic (Ames test). Jarilla flowers could be considered as a new dietary supplement that could help to prevent pathologies associated with oxidative stress and the polyphenolic extract obtained from them could be considered as a standardized phytotherapeutic product with antimicrobial, antioxidant and anti-inflammatory activities. The aim of this work was to determine the pigments responsible for the yellow color of the flowers of Z. punctata and to evaluate the functional properties of the polyphenolic extract of the flowers. The toxicity (Artemia salina) and mutagenic activity (Ames test) of the extract were also evaluated. PMID:26197533

  7. Evaluación ambiental del río San Juan de Santiago de Cuba por exposición bioacumulativa a metales pesados Environmental evaluation of San Juan river in Santiago de Cuba due to bioaccumulative exposure to heavy metals

    Directory of Open Access Journals (Sweden)

    George Argota Pérez

    2012-08-01

    Full Text Available Se evaluó la salud ambiental del río San Juan de Santiago de Cuba, expuesto a metales pesados, mediante la utilización de la especie biorreguladora Gambusia punctata. Se analizaron 271 individuos adultos clasificados por sexo, para lo cual se escogieron los intervalos de clases de edades 2,1-3,0 y 3,1-4,0 cm, según la longitud total, respectivamente. La mezcla ácida aplicada estuvo compuesta por ácido perclórico - ácido sulfúrico; (7:1 ácido nítrico para determinar los niveles bioacumulados en hígado, branquias y cerebro como órganos diana. Los metales analizados: cobre, zinc, plomo y cadmio, se cuantificaron mediante espectrometría por plasma inductivamente acoplado con vista axial. Se hallaron diferencias significativas (pThe environmental health of San Juan river in Santiago de Cuba, exposed to heavy metals was evaluated by means of the bioregulatory species Gambusia punctata. Two hundred and seventy five adults were analyzed and classified by sex, for whom the intervals of age classes 2.1-3.0 and 3.1-4.0 cm were chosen, according to the total longitude, respectively. The applied acid mixture was composed by perchloric acid - sulphuric acid; (7:1 nitric acid to determine the bioaccumulated levels in liver, branchiae and brain as target organs. The analyzed metals: copper, zinc, lead and cadmium, were quantified by means of spectrometry by plasma inductively coupled with axial view. There were significant differences (p <0.05 in the bioaccumulated values between intervals and sexes. It was concluded that the levels of bioaccumulated heavy metals overcame the permissible values, so that they represent an environmental risk in the quality of those waters.

  8. Phylogenetic relationships of five uncommon species of Lasiosphaeria and three new species in the Helminthosphaeriaceae (Sordariomycetes).

    Science.gov (United States)

    Miller, Andrew N; Huhndorf, Sabine M; Fournier, Jacques

    2014-01-01

    In an ongoing effort to monograph the genus Lasiosphaeria, it is desirable to obtain estimates of the phylogenetic relationships for five uncommon species, L. coacta, L. munkii, L. punctata, L. sphagnorum and L. stuppea. Three additional species previously placed in Lasiosphaeria, Echinosphaeria canescens, Hilberina caudata and Ruzenia spermoides, also were included in this study as well as three undescribed species. These species were believed to have relations elsewhere based on various ambiguous morphological characters, so an independent dataset from one or more genes was used to resolve their phylogenetic affinities. Sequences from the nuclear ribosomal 28S large subunit (LSU) and β-tubulin genes were generated for these taxa. Maximum likelihood and Bayesian analyses indicated these taxa form a well supported monophyletic group with members of the Helminthosphaeriaceae and therefore, should be transferred out of Lasiosphaeria. Except for Helminthosphaeria gibberosa, Hilberina elegans, Ruzenia spermoides and Synaptospora plumbea, all taxa within this clade possess ascomata with distinct thick-walled setae. Based on a combination of morphological and molecular data, Helminthosphaeria tomaculum, Helminthosphaeria triseptata and Hilberina robusta are described as new and four new combinations are proposed: Helminthosphaeria ludens, Hel. stuppea, Hilberina punctata and H. sphagnorum. Ten new combinations are proposed based on morphological data: Echinosphaeria heterostoma, Helminthosphaeria flavocompta, Hel. gibberosa, Hel. heterotricha, Hilberina breviseta, H. elegans, H. foliicola, H. meznaensis, H. moseri and H. rhynchospora. Lasiosphaeria coacta is placed in synonymy with Hel. ludens and the previous transfer of Hilberina munkii is accepted. Synaptospora plumbea was found to belong in the family. Illustrations are provided for most Helminthosphaeriaceae taxa seen in this study. PMID:24871607

  9. Estimativa do tamanho de duas espécies de ciclídeos (Osteichthyes, Perciformes predados por Lontra longicaudis (Olfers (Carnivora, Mustelidae, através de análise das escamas Size estimation of two species of Ciclids (Ostheichthyes, Perciformes predated by Lontra longicaudis (Olfers (Carnivora, Mustelidae through scale analysis

    Directory of Open Access Journals (Sweden)

    Carlos Benhur Kasper

    2004-09-01

    Full Text Available O presente estudo foi realizado no Vale do Taquari, região central do Estado do Rio Grande do Sul, sul do Brasil. Entre dezembro de 2001 e dezembro de 2002 foi realizado um estudo sobre a predação de Cichlidae por Lontra longicaudis (Olfers, 1818 através de análise das escamas encontradas nas fezes de lontra. Foi identificada a predação sobre Gimnogeophagus labiatus (Hensel, 1870 e Crenicichla punctata Hensel, 1870, e a ocorrência destas espécies de peixe na dieta é mais elevada do que sua disponibilidade relativa no ambiente. Foi encontrada uma correlação positiva entre o tamanho do peixe e das escamas, permitindo a construção de uma curva de regressão para estimar o tamanho dos peixes predados baseado no tamanho das escamas encontradas nas fezes de lontra. Neste estudo, os ciclídeos mais frequentemente predados variaram no comprimento entre 100 e 150 mm e no peso entre 22 e 37 g.The present study was carried out in the Taquari Valley, central region of Rio Grande do Sul State, Southern Brazil. Between December 2001 and December 2002 a study about the predation of Cichlidae by Lontra longicaudis (Olfers, 1818 was performed through scale analysis found in otter scats. Predation was indentified about Gimnogeophagus labiatus (Hensel, 1870 and Crenicichla punctata Hensel, 1870, and the occurence of these fish species in the diet were higher than the relative availability in the environment. A positive correlation between fish and scale sizes was found, allowing to build a regression curve to estimate the size of predated fish, based on scales found in otter scats. In this study ciclids most frequently predated varied in lenght from 100 and 150 mm and in weight from 22 to 37,6 g.

  10. 冰灾后城市森林不同恢复类型土壤微生物特性及其对土壤因子的响应%Soil Microbial Characteristics and Its Response to Soil Factors in Vegetation Restoration Types in Urban Forest Destroyed by Ice Storm

    Institute of Scientific and Technical Information of China (English)

    陈建国; 田大伦; 闫文德; 项文化; 方晰; 陈仕栋

    2011-01-01

    为探究冰灾后城市森林不同植被恢复类型土壤微生物的演变特征,对长沙天际岭林场冰灾后3种恢复植被的栾树人工纯林、蕨草地、针阔混交林冬夏两季的土壤微生物数量、微生物活度、土壤理化性质及其相互关系进行了比较研究.结果表明,夏初不同植被恢复类型0-15 cm土层土壤微生物活度以针阔混交林最高(0.302),蕨草地次之(0.261),栾树人工纯林最低(0.192);3类植被中栾树人工纯林土壤冬季放线菌活力最弱,夏季细菌活力最弱;栾树人工纯林、针阔混交林土壤微生物生命活动冬季以真菌、细菌为主,夏季以真菌、放线菌为主,蕨草地无论冬夏土壤微生物活动都以真菌和细菌为主,放线菌活动较弱;在土壤微生物生命活动旺盛时期,土壤有机碳含量与土壤微生物数量呈显著正相关,同时土壤微生物能忍受较高的土壤含水量、要求较低的土壤容重,在土壤微生物生命活动衰弱时期土壤有机碳含量与微生物数量不相关或呈负相关,此时土壤微生物要求较低的土壤含水量、较高的土壤容重.总之,栾树人工纯林土壤微生物生命活力比自然恢复的常绿次生针阔混交林及以姬蕨为主的蕨草地差,而后两者表层土壤微生物性状相比针阔混交林地优于蕨草地.%To study the evolving soil microbial characteristics in restoring urban forest destroyed by ice storm, an investigation had been conducted in three vegetation restoration types (Koelreuteria paniculata pure forest, Hypolepis punctata rangeland, pine and broad leaf mixed forest) in Tianjiling forest centre, Changsha. The soil quantities of microbe, soil microbial activity, the physic-chemical characteristics in the soil, and among which relation for each vegetation restoration types were measured/calculated and compared. The results showed as followed. The soil microbial activity for the layer soil of 0-15 em in pine and broad leaf

  11. EcmPred: Prediction of extracellular matrix proteins based on random forest with maximum relevance minimum redundancy feature selection

    KAUST Repository

    Kandaswamy, Krishna Kumar Umar

    2013-01-01

    The extracellular matrix (ECM) is a major component of tissues of multicellular organisms. It consists of secreted macromolecules, mainly polysaccharides and glycoproteins. Malfunctions of ECM proteins lead to severe disorders such as marfan syndrome, osteogenesis imperfecta, numerous chondrodysplasias, and skin diseases. In this work, we report a random forest approach, EcmPred, for the prediction of ECM proteins from protein sequences. EcmPred was trained on a dataset containing 300 ECM and 300 non-ECM and tested on a dataset containing 145 ECM and 4187 non-ECM proteins. EcmPred achieved 83% accuracy on the training and 77% on the test dataset. EcmPred predicted 15 out of 20 experimentally verified ECM proteins. By scanning the entire human proteome, we predicted novel ECM proteins validated with gene ontology and InterPro. The dataset and standalone version of the EcmPred software is available at http://www.inb.uni-luebeck.de/tools-demos/Extracellular_matrix_proteins/EcmPred. © 2012 Elsevier Ltd.

  12. Hyena disease (premature physeal closure) in calves due to overdose of vitamins A, D3, E.

    Science.gov (United States)

    Yamamoto, Kenichi; Sadahito, Kobayashi; Yoshikawa, Makoto; Nobuyuki, Onizuka; Mikami, Osamu; Yamada, Manabu; Nakamura, Kikuyasu; Yasuyuki, Nakajima

    2003-03-01

    Holstein suckling calves on a farm manifested severe emaciation, generalized alopecia, dome-like cranial deformation, and high mortality (Case 1). Metaphyseal growth plates of the femur were achondroplastic; segmented, partially resorped, and replaced with immature bony trabeculae containing degenerated chondrocytes. The skull was thin and partially replaced with connective tissue. Diffuse and severe fatty degeneration was observed in the hepatic stellate (Ito') cells. After 6 mo, surviving calves manifested unthrifty with short and irregular hindquarters (Case 2). The metaphyseal growth plates were poorly formed, irregular, partially disappeared centrally, and often sealed with thin bony trabeculae. The cartilage matrix was not homogeneous but was finely fibrous, and chondrocytes were flat and degenerated. The bone lesion was diagnosed as chondrodysplasia due to premature physeal closure. These calves had been administered excessive amounts of vitamins A, D3 and E, and blood chemistry of acute case showed hypervitaminosis A and E. Case I demonstrated acute disease, while Case 2 demonstrated chronic sequelae. Hypervitaminosis A was the suspected cause. PMID:12678294

  13. Role of FGF/FGFR signaling in skeletal development and homeostasis:learning from mouse models

    Institute of Scientific and Technical Information of China (English)

    Nan Su; Min Jin; Lin Chen

    2014-01-01

    Fibroblast growth factor (FGF)/fibroblast growth factor receptor (FGFR) signaling plays essential roles in bone development and diseases. Missense mutations in FGFs and FGFRs in humans can cause various congenital bone diseases, including chondrodysplasia syndromes, craniosynostosis syndromes and syndromes with dysregulated phosphate metabolism. FGF/FGFR signaling is also an important pathway involved in the maintenance of adult bone homeostasis. Multiple kinds of mouse models, mimicking human skeleton diseases caused by missense mutations in FGFs and FGFRs, have been established by knock-in/out and transgenic technologies. These genetically modified mice provide good models for studying the role of FGF/FGFR signaling in skeleton development and homeostasis. In this review, we summarize the mouse models of FGF signaling-related skeleton diseases and recent progresses regarding the molecular mechanisms, underlying the role of FGFs/FGFRs in the regulation of bone development and homeostasis. This review also provides a perspective view on future works to explore the roles of FGF signaling in skeletal development and homeostasis.

  14. Phenotypic expressions of a Gly154Arg mutation in type II collagen in two unrelated patients with spondyloepimetaphyseal dysplasia (SEMD)

    Energy Technology Data Exchange (ETDEWEB)

    Kaitila, I.; Marttinen, E. [Helsinki Univ. Hospital (Finland); Koerkkoe, J.; Ala-Kokko, L. [Thomas Jefferson Univ., Philadelphia, PA (United States)

    1996-05-03

    Type II collagenopathies consist of chondrodysplasia ranging from lethal to mild in severity. A large number of mutations has been found in the COL2A1 gene. Glycine substitutions have been the most common types of mutation. Genotype-phenotype correlations in type II collagenopathies have not been established, partly because of insufficient clinical and radiographic description of the patients. We found a glycine-to-arginine substitution at position 154 in type II collagen in two unrelated isolated propositi with spondyloepimetaphyseal dysplasia and provide a comparative clinical and radiographic analysis from birth to young adulthood for this condition. The clinical phenotype was disproportionate short stature with varus/valgus deformities of the lower limbs requiring corrective osteotomies, and lumbar lordosis. The skeletal radiographs showed an evolution from short tubular bones, delayed epiphyseal development, and mild vertebral involvement to severe metaphyseal dysplasia with dappling irregularities, and hip {open_quotes}dysplasia.{close_quotes} The metaphyseal abnormalities disappeared by adulthood. 27 refs., 11 figs., 1 tab.

  15. Achondroplasia Presenting with Pneumonia in a Two Months Old Boy

    Directory of Open Access Journals (Sweden)

    Huseyin Bilgin

    2014-03-01

    Full Text Available Achondroplasia is one of the common chondrodysplasias with an inheritance is autosomal dominant, but in around 85% the phenotype is the result of a new mutation. Achondroplasia develops as a result of dysplasia of enchondral formation due to the mutation of fibroblast growth factor receptor 3. A 2-month-old boy was referred to the our hospital with cough and fever. Craniofacially the head appeared large and also frontal bossing and depressed nasal bridge was demonstrated. Narrow lumbar interpedicular distances, normal trunk length, short-wide pelvis, micromelic upper extremities and rhizomelic lower extremities were seen on x-ray examination. The clinically and radiographically diagnosis of achondroplasia with heart failure secondary to pneumonia was performed. Achondroplasia, presenting with respiratory disorders and short limb should be differentiated from metatropic dysplasia and campomelic dysplasia. Achondroplasia may had similar findings with other dwarfism and differentiate diagnosis from other achondroplasia like diseases needs team work which includes pediatry, radiology and medical genetic for better patient care and family counseling.

  16. Generation of Fgfr3 Conditional Knockout Mice

    Directory of Open Access Journals (Sweden)

    Nan Su, Xiaoling Xu, Cuiling Li, Qifen He, Ling Zhao, Can Li, Siyu Chen, Fengtao Luo, Lingxian Yi, Xiaolan Du, Haiyang Huang, Chuxia Deng, Lin Chen

    2010-01-01

    Full Text Available Fibroblast growth factor receptor 3 (FGFR3, highly conserved in both humans and murine, is one of key tyrosine kinase receptors for FGF. FGFR3 is expressed in different tissues, including cartilage, brain, kidney, and intestine at different development stages. Conventional knockout of Fgfr3 alleles leads to short life span, and overgrowth of bone. In clinic, human FGFR3 mutations are responsible for three different types of chondrodysplasia syndromes including achondroplasia (ACH, hypochondroplasia (HCH and thanatophoric dysplasia (TD. For better understanding of the roles of FGFR3 in different tissues at different stages of development and in pathological conditions, we generated Fgfr3 conditional knockout mice in which loxp sites flank exons 9-10 in the Fgfr3 allele. We also demonstrated that Cre-mediated recombination using Col2a1-Cre, a Cre line expressed in chondrocyte during bone development, results in specific deletion of the gene in tissues containing cartilage. This animal model will be useful to study distinct roles of FGFR3 in different tissues at different ages.

  17. C-Type Natriuretic Peptide Analog as Therapy for Achondroplasia.

    Science.gov (United States)

    Legeai-Mallet, Laurence

    2016-01-01

    Fibroblast growth factor receptor 3 (FGFR3) is an important regulator of bone formation. Gain-of-function mutations in the FGFR3 gene result in chondrodysplasias which include achondroplasia (ACH), the most common form of dwarfism, in which skull, appendicular and axial skeletons are affected. The skeletal phenotype of patients with ACH showed defective proliferation and differentiation of the chondrocytes in the growth plate cartilage. Both endochondral and membranous ossification processes are disrupted during development. At cellular level, Fgfr3 mutations induce increased phosphorylation of the tyrosine kinase receptor FGFR3, which correlate with an enhanced activation of its downstream signaling pathways. Potential therapeutic strategies have emerged for ACH. Several preclinical studies have been conducted such as the C-type natriuretic peptide (CNP) analog (BMN111), intermittent parathyroid hormone injections, soluble FGFR3 therapy, and meclozine and statin treatments. Among the putative targets to antagonize FGFR3 signaling, CNP (or BMN111) is one of the most promising strategies. BMN111 acts as a key regulator of longitudinal bone growth by downregulating the mitogen-activated protein kinase pathway, which is activated as a result of a FGFR3 gain-of-function mutation. Preclinical studies showed that BMN111 treatment led to a large improvement in skeletal parameters in Fgfr3Y367C/+ mice mimicking ACH. In 2014, a clinical trial (phase 2) of BMN111 in pediatric patients with ACH has started. This first clinical trial marks the first big step towards real treatment for these patients. PMID:26684019

  18. Dysplastic histogenesis of cartilage growth plate by alteration of sulphation pathway: a transgenic model.

    Science.gov (United States)

    Cornaglia, Antonia Icaro; Casasco, Andrea; Casasco, Marco; Riva, Federica; Necchi, Vittorio

    2009-01-01

    Mutations in the diastrophic dysplasia sulphate transporter (dtdst) gene causes different forms of chondrodysplasia in the human. The generation of a knock-in mouse strain with a mutation in dtdst gene provides the basis to study developmental dynamics in the epiphyseal growth plate and long bone growth after impairment of the sulphate pathway. Our microscopical and histochemical data demonstrate that dtdst gene impairment deeply affects tissue organization, matrix structure, and cell differentiation in the epiphyseal growth plate. In mutant animals, the height of the growth plate was significantly reduced, according to a concomitant decrease in cell density and proliferation. Although the pathway of chondrocyte differentiation seemed complete, alteration in cell morphology compared to normal counterparts was detected. In the extracellular matrix, it we observed a dramatic decrease in sulphated proteoglycans, alterations in the organization of type II and type X collagen fibers, and premature onset of mineralization. These data confirm the crucial role of sulphate pathway in proteoglycan biochemistry and suggest that a disarrangement of the extracellular matrix may be responsible for the development of dtdts cartilage dysplasia. Moreover, we corroborated the concept that proteoglycans not only are structural components of the cartilage architecture, but also play a dynamic role in the regulation of chondrocyte growth and differentiation. PMID:19637059

  19. Novel IFT122 mutations in three Argentinian patients with cranioectodermal dysplasia: Expanding the mutational spectrum.

    Science.gov (United States)

    Moosa, Shahida; Obregon, Maria Gabriela; Altmüller, Janine; Thiele, Holger; Nürnberg, Peter; Fano, Virginia; Wollnik, Bernd

    2016-05-01

    Cranioectodermal dysplasia (CED), also known as Sensenbrenner syndrome, is an autosomal recessive ciliary chondrodysplasia characterized by a recognizable craniofacial gestalt, skeletal abnormalities, and ectodermal features. To date, four genes have been shown to underlie the syndrome, namely, IFT122 (WDR10), WDR35 (IFT121), IFT43 (C14orf179), and WDR19 (IFT144). Clinical characterization of a larger cohort of patients with CED has been undertaken previously. Nevertheless, there are too few molecularly confirmed patients reported in the literature to determine precise genotype-phenotype correlations. To date, biallelic IFT122 mutations have been described in only five families. We therefore studied three unrelated Argentinian patients with typical features of CED using a 4813 next-generation sequencing (NGS) gene panel, which we call the "Mendeliome." The three patients had different, novel, compound heterozygous mutations in IFT122. Consequently, we compared these three patients to those previously described with IFT122 mutations. Thus, our report serves to add 6 novel mutations to the IFT122 mutation spectrum and to contribute to the IFT122-related clinical characterization. © 2016 Wiley Periodicals, Inc. PMID:26792575

  20. CCN1 Regulates Chondrocyte Maturation and Cartilage Development.

    Science.gov (United States)

    Zhang, Yongchun; Sheu, Tzong-Jen; Hoak, Donna; Shen, Jie; Hilton, Matthew J; Zuscik, Michael J; Jonason, Jennifer H; O'Keefe, Regis J

    2016-03-01

    WNT/β-CATENIN signaling is involved in multiple aspects of skeletal development, including chondrocyte differentiation and maturation. Although the functions of β-CATENIN in chondrocytes have been extensively investigated through gain-of-function and loss-of-function mouse models, the precise downstream effectors through which β-CATENIN regulates these processes are not well defined. Here, we report that the matricellular protein, CCN1, is induced by WNT/β-CATENIN signaling in chondrocytes. Specifically, we found that β-CATENIN signaling promotes CCN1 expression in isolated primary sternal chondrocytes and both embryonic and postnatal cartilage. Additionally, we show that, in vitro, CCN1 overexpression promotes chondrocyte maturation, whereas inhibition of endogenous CCN1 function inhibits maturation. To explore the role of CCN1 on cartilage development and homeostasis in vivo, we generated a novel transgenic mouse model for conditional Ccn1 overexpression and show that cartilage-specific CCN1 overexpression leads to chondrodysplasia during development and cartilage degeneration in adult mice. Finally, we demonstrate that CCN1 expression increases in mouse knee joint tissues after meniscal/ligamentous injury (MLI) and in human cartilage after meniscal tear. Collectively, our data suggest that CCN1 is an important regulator of chondrocyte maturation during cartilage development and homeostasis. © 2015 American Society for Bone and Mineral Research. PMID:26363286

  1. Mode of reproduction of Brazilian species of Adesmia (Leguminosae

    Directory of Open Access Journals (Sweden)

    Solange B. Tedesco

    2000-06-01

    Full Text Available Mode of reproduction was studied in 15 species of Adesmia DC. (Leguminosae. In six species, three treatments were used: mutual pollination, mechanical stimulation and control. Fifty-four plants of these six species were grown in a greenhouse, individually isolated in nylon screen boxes. Flowers were labelled and submitted to the different treatments. In addition, the frequency of spontaneous self-pollination in the absence of pollinators was studied in 200 plants of nine other species. These 200 plants were kept in a greenhouse, which avoided contact with any possible pollinator. Adesmia bicolor, A. muricata, A. punctata and A. riograndensis produced seed both by cross- and self-pollination. Adesmia punctata and A. riograndensis need mechanical stimulation for self-pollination. Adesmia incana reproduced by self-pollination; however, the possibility of cross-pollination cannot be totally ruled out. Adesmia tristis reproduced mainly by cross-pollination and a mechanism of self-incompatibility is suggested. Among the nine species that were not exposed to pollinators, A. securigerifolia produced a large amount of seed, indicating that it is a self-pollinating species. Adesmia arillata, A. araujoi, A. ciliata, A. psoraleoides, A. rocinhensis, A. reitziana, A. sulina and A. vallsii did not produce any seed under the experimental conditions, suggesting that they are cross-pollinated or that they need mechanical stimulation to reproduce.Foram estudadas 15 espécies do gênero Adesmia DC. (Leguminosae, quanto ao modo de reprodução. Em seis espécies do gênero Adesmia, o modo de reprodução foi determinado através de três tratamentos: polinização mútua, estímulo mecânico e controle. As 54 plantas submetidas aos tratamentos foram cultivadas em casa de vegetação e mantidas isoladas individualmente, através de armações de tela de náilon. As flores foram marcadas e submetidas aos distintos tratamentos. Adicionalmente, foram observadas 200

  2. Toxic effects of herbicide paraquat on different species of Lemnaceae%除草剂百草枯对浮萍科不同植物的毒性效应比较

    Institute of Scientific and Technical Information of China (English)

    王林林; 张光富; 何谐; 葛峰; 周军英

    2013-01-01

    为揭示除草剂对3种浮萍科植物的毒害作用,采用半数抑制浓度(IC50)和慢性值(ChV)分析并比较百草枯对其急性和慢性毒性效应.结果表明:处理96 h后,紫萍、兰氏萍和浮萍叶状体的IC50值依次为17.37、6.16和4.75 μg·L-1,百草枯对浮萍科不同植物的急性毒性作用存在明显差别(P<0.05);3种植物叶绿素含量均随百草枯浓度的升高而显著下降,处理4d后,两者呈显著负相关(P<0.05);紫萍的ChV为3.05 μg·L-1,兰氏萍和浮萍的ChV均为0.96 μg·L-1;根据叶状体指标,紫萍的ChV为10.780 μg·L-1,浮萍的ChV仅为3.410 μg·L-1,表明不论根据叶状体个数还是叶绿素含量,百草枯对浮萍的长期毒害效应均大于紫萍.因此,百草枯对3种浮萍科植物的毒害作用存在明显的分类学效应、浓度-剂量效应和时间滞后效应.%To explore the toxic effects of herbicides on different species of Lemnaceae,the median inhibition concentration (IC50) and chronic value (ChV) of paraquat to Spirodela polyrhiza,Landoltia punctata,and Lemna minor were measured,with the acute and chronic toxic effects of paraquat on these three Lemnaceae species compared.After treated for 96 hours,the IC50 value of paraquat to S.polyrhiza,L.punctata,and L.minor was 17.37,6.16,and 4.75 μg · L-1,respectively,and there existed significant differences among the acute toxicity (P<0.05).The chlorophyll content of the three species all decreased significantly with increasing paraquat concentration,and after treated for four days,a significant negative correlation was observed between the chlorophyll content and paraquat concentration (P<0.05).The ChV of paraquat to S.polyrhiza was 3.050 μg · L-1,and while that to both L.punctata and L.minor was 0.964 μg ·L-1.However,in light of frond features,the ChV of paraquat to S.polyrhiza was 10.780 μg ·L-1,while that to L.minor was only 3.410 μg · L-1,implying that the chronic toxic effect of paraquat to L

  3. Disponibilidade de larvas infectantes de nematódeos gastrintestinais parasitas de ovinos deslanados no semi-ârido pernambucano Seasonal availability of sheep gastrointestinal nematode infection in the semi-arid areas of Pernambuco state

    Directory of Open Access Journals (Sweden)

    Terezinha Padilha Charles

    1995-01-01

    Full Text Available A cada período médio de 30 dias (25 a 56 dias compreendidos entre 08 de janeiro de 1988 e 11 de maio de 1990, dois ovinos deslanados, machos, com idade entre três a seis meses de idade, livres de vermes, eram colocados para pastejar juntamente com um rebanho naturalmente infectado. Após o período de pastejo, os animais permaneciam estabulados por 14-21 dias quando eram submetidos à necropsia para coleta, contagem e identificação dos nematódeos adquiridos. Haemonchus contortus foi o nematódeo mais prevalente (80,0%, seguido do Trichostrongylus spp. (T. axei e T. colubriformis e Oxperia spp. (C. pectinata e C. punctata presentes em 72,0% dos animais examinados, Oesophagostomum radiatum e Stcoagyloides papillosus estavam presentes em 56,0% e 38,0% dos animais examinados, respectivamente. O número médio de H. contortus variou de 510 na estação seca a 2157 na chuvosa, sendo o número médio de larvas de quarto estágio desse nematódeo de 499 na estação chuvosa (23% do total adquirido no período e 416 na seca (81,6% do total médio adquirido no período. O número médio de Trichostrongylus spp. (T. axei e T. colubriformis, Cooperia spp. (C. pectinata and C. punctata, O. columbianum e S. papillous variou de 89, 89, 17 e 5 na estação seca a 497, 109, 93 e 284 na chuvosa, respectivamente. A transmissão das várias espécies ocorreu principalmente no meado da estação chuvosa até o início da estação seca.During each of 25-56 days periods from January 08, 1988 to May 05, 1990, two male sheep three to six month old, shown to be free of gastrointestinal nematode infection, were grazed with a flock of naturally infected sheep (tracer sheep. At the end of each grazing period, these sheep were placed on a cement floored pen and were maintained there for two to three weeks prior to necropsy and examination for gastrointestinal nematodes. Haemonchus contortus was the most prevalent nematode found (80%. The mean number of this nematode

  4. Gafanhotos (Orthoptera, Acridoidea em áreas de cerrados e lavouras na Chapada dos Parecis, Estado de Mato Grosso, Brasil Grasshoppers (Orthoptera, Acridoidea in native savanna and crop areas in Chapada dos Parecis, Mato Grosso State, Brazil

    Directory of Open Access Journals (Sweden)

    Wanderlei Dias Guerra

    2012-06-01

    Full Text Available Gafanhotos (Orthoptera, Acridoidea em áreas de cerrados e lavouras na Chapada dos Parecis, Estado de Mato Grosso, Brasil. Foi determinada a composição e abundância de espécies de gafanhotos usando amostragem com rede entomológica durante 3 anos de estudo na Chapada dos Parecis, estado de Mato Grosso. O levantamento foi feito em áreas de lavouras e com vegetação ainda nativa (cerrados com, respectivamente, 56 e 59 locais inventariados em cada ambiente. Foram coletados 3.031 indivíduos de gafanhotos de 64 espécies distribuídas entre as famílias e subfamílias: Acrididae (49: Gomphocerinae (21, Ommatolampinae (10, Melanoplinae (6, Acridinae (4 Leptysminae (3, Copiocerinae (3, Proctolabinae (1 e Cyrtacanthacridinae (1; Romaleidae (1: Romaleinae (13 e Ommexechidae (1: Ommexechinae (2, além de 1550 ninfas. A diversidade de espécies foi maior no cerrado (61 do que nas lavouras (16, ocorrendo o inverso com relação à abundância onde as espécies Baeacris punctulatus (Thunberg, 1824 e Orphulella punctata (De Geer, 1773 predominaram representando 49,5% do total de indivíduos coletados em toda a Chapada dos Parecis e, juntas, somam 78,8% da abundância registrada nas áreas de lavouras e tem potencial de se tornarem pragas.Grasshoppers (Orthoptera, Acridoidea in native savanna and crop areas in Chapada dos Parecis, Mato Grosso State, Brazil. We determined the composition and abundance of grasshoppers using sweep net sampling during three years at the Parecis Plateau, State of Mato Grosso, Brazil. The survey was done in areas with crops and native vegetation (savanna with, respectively, 56 and 59 sites available in each environment. 3.031 individuals of grasshoppers were collected from 64 species distributed among the following families and subfamilies: Acrididae (49: Gomphocerinae (21, Ommatolampinae (10, Melanoplinae (6, Acridinae (4 Leptysminae (3, Copiocerinae (3, Proctolabinae (1 and Cyrtacanthacridinae (1; Romaleidae (1: Romaleinae

  5. Relações de tamanho e peso das grandes medusas (Cnidaria do litoral do Paraná, Sul do Brasil Size-weight relationship among macromedusae (Cnidaria of Paraná coast Southern Brazil

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    Miodeli Nogueira Jr

    2006-12-01

    Full Text Available Relações de tamanho e peso são dados básicos importantes para facilitar estimativas de estoques, de monitoramentos contínuos e de larga escala das espécies, além de permitir a obtenção de valores de biomassa a partir da distribuição de tamanho. Apesar destes dados serem básicos e de reconhecida importância ecológica e sócio-econômica, estudos biométricos das grandes medusas do litoral brasileiro ainda inexistem. Apresenta-se, neste estudo, as relações de tamanho e peso das seis espécies de macromedusas mais comuns no litoral paranaense: Tamoya haplonema Müller, 1859 e Chiropsalmus quadrumanus (Müller, 1859 - Cubozoa; Olindias sambaquiensis Müller, 1861 - Hydrozoa; Chrysaora lactea Eschscholtz, 1829, Lychnorhiza lucerna Haeckel, 1880 e Phyllorhiza punctata von Lendenfeld, 1884 - Scyphozoa. Foram utilizadas equações do tipo Y = aXb onde, Y = peso úmido; X = diâmetro da umbrela (com exceção de T. haplonema, para a qual utilizou-se a medida de altura umbrelar; a e b são os parâmetros estimados para cada população. A maioria das espécies estudadas apresentou alometria negativa, uma vez que b era menor que 3 na maioria dos casos, variando entre 2,415 e 3,028.Size-weight relationships are important for population stock estimates, continuous and large-scale population monitoring, as well as permitting the estimation of population biomass from size-class distribution. While these data are basic and useful for ecological and economical analyses, such estimates for Brazilian jellyfish do not exist. Here we present size-weight relationships for the six most common species of large medusae in the coast of Paraná, southern Brazil: Tamoya haplonema Müller, 1859 and Chiropsalmus quadrumanus (Müller, 1859 - Cubozoa; Olindias sambaquiensis Müller, 1861 - Hydrozoa; Chrysaora lactea Eschscholtz, 1829, Lychnorhiza lucerna Haeckel, 1880 and Phyllorhiza punctata von Lendenfeld, 1884 - Scyphozoa. A regression of the form Y = a

  6. Infecções helmínticas em um rebanho leiteiro na região Campo das Vertentes de Minas Gerais Helminth infection pattern of a dairy herd in Campo das Vertentes region in Minas Gerais state

    Directory of Open Access Journals (Sweden)

    R.N. Araujo

    2005-09-01

    Full Text Available Bezerros traçadores foram utilizados para avaliar a contaminação sazonal das pastagens por helmintos gastrintestinais e pulmonares em uma propriedade de exploração leiteira na região Campo das Vertentes, Minas Gerais. Os animais se infectaram durante todos os meses do ano. As maiores cargas parasitárias foram recuperadas no período chuvoso (setembro - abril, e o pico foi observado em abril-maio, final do período chuvoso. As espécies recuperadas foram Cooperia punctata, C. spatulata, Haemonchus contortus, H. similis, Oesophagostomum radiatum, Trichostrongylus axei, T. colubriformis, Trichuris discolor, T. globulosa, Dictyocaulus viviparus e Agriostomum vryburgi. Para avaliar a dinâmica das infecções, foi acompanhado um grupo de vacas com seus bezerros lactantes no período de janeiro de 1999 a fevereiro de 2000. Os valores da contagem de ovos por grama de fezes (OPG dos bezerros apresentaram dois picos, em maio de 1999 e fevereiro de 2000, enquanto o pico da contagem de OPG das vacas ocorreu em julho e agosto de 2000. Nas coproculturas, os gêneros de maior ocorrência foram Cooperia e Haemonchus nos bezerros, e Haemonchus e Trichostrongylus nas vacas.Tracer calves used to monitor contamination of pasture with pulmonary and gastrointestinal nematode helminths on a dairy farm were infected throughout the year. The highest parasite burdens were observed during the rainy season (September - April. However, the highest peak occurred in April and May 1999, the end of the rainy season. Helminth species recovered from the calves were Cooperia punctata, C. spatulata, Haemonchus contortus, H. similis, Oesophagostomum radiatum, Trichostrongylus axei, T. colubriformis, Trichuris discolor, T. globulosa, Dictyocaulus viviparus and Agriostomum vryburgi. A group of lactating cows and their accompanying calves were followed during the period from January 1999 to February 2000 to evaluate the dynamics of their infection. Parasite eggs per gram of

  7. A multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion.

    Science.gov (United States)

    de Melo Pereira, Gilberto Vinícius; Magalhães, Karina Teixeira; Lorenzetii, Emi Rainildes; Souza, Thiago Pereira; Schwan, Rosane Freitas

    2012-02-01

    This study used a multiphasic approach, characterized by the simultaneous use of culture-dependent and culture-independent methods, to investigate endophytic bacterial communities in strawberry (Fragaria ananassa) fruit. A total of 92 bacterial endophytes were isolated and initially grouped by their repetitive extragenic palindromic (rep)-PCR banding pattern and biochemical features. Phylogenetic analysis of the 16S rRNA gene sequences of 45 representatives showed that the isolates belonged to the species Bacillus subtilis (eight isolates), Bacillus sp. (seven isolates), Enterobacter sp. (seven isolates), Enterobacter ludwigii (six isolates), Lactobacillus plantarum (six isolates), Pseudomonas sp. (five isolates), Pantoea punctata (three isolates), and Curtobacterium citreum (three isolates). Nucleic acids were extracted from the strawberry fruit and subjected to 16S rRNA gene directed polymerase chain reaction denaturing gradient gel electrophoresis (16S rRNA PCR-DGGE). The species B. subtilis, Enterobacter sp., and Pseudomonas sp. were detected both by isolation and DGGE. The DGGE fingerprints of total bacterial DNA did not exhibit bands corresponding to several of the representative species isolated in the extinction dilution (L. plantarum, C. citreum, and P. punctata). In contrast, bands in the DGGE profile that were identified as relatives of Arthrobacter sp. and one uncultivable Erythrobacter sp. were not recovered by cultivation techniques. After isolation, the nitrogen fixation ability and the in vitro production of indole-3-acetic acid (IAA) equivalents and siderophores were evaluated. A high percentage of isolates were found to possess the ability to produce siderophores and IAA equivalents; however, only a few isolates belonging to the genera Pseudomonas and Enterobacter showed the ability to fix nitrogen. Plant growth promotion was evaluated under greenhouse conditions and revealed the ability of the Bacillus strains to enhance the number of leaves

  8. Repellent Activity of Extracts of Wild Rice Species against Panonychus citri and Aphis citricola in Associated with Esterase Isoenzyme in Insests

    Institute of Scientific and Technical Information of China (English)

    WAN Shu-qing; LIU Xiang-fa; FENG Guo-zhong; PAN Da-jian

    2006-01-01

    Six species of wild rice with different ecophenotypes including Oryza grandiglumis (E6-1, E6-3 / 6-4), O. minuta (E13-9,E13-13), O. officinalis (E15-8, E15-13), O. punctata (E16-1, E16-3, E1 6-13), O. granulata (E7-4), and O. latifolia (101392, E9-1, E9-10)were extracted with methnol and the repellent activity of the extracts against the two insects Aphis citricola and Panonychus citri were studied. The extracts of O. officinalis E15-8 showed higher repellent rate to the two insects than those of the other species. The repellent rates of the extracts of E15-8 to P. citriand A. citricola were 83.26% and 87.86% at 5×104 μg/mL in 24 h and 87.95% and 82.43% in 48 h, respectively. The extracts of O. officinalis E15-8 had the effect of inhibition to the esterase of the two insects.

  9. Molecular characterization of hard and soft ticks from Romania by sequences of the internal transcribed spacers of ribosomal DNA.

    Science.gov (United States)

    Chitimia, L; Lin, R Q; Cosoroaba, I; Braila, P; Song, H Q; Zhu, X Q

    2009-10-01

    In the present study, four hard tick species and one soft tick species, namely, Dermacentor marginatus, Haemaphysalis punctata, Haemaphysalis parva, Ixodes ricinus, and Dermanyssus gallinae, from south-western Romania were characterized genetically by the first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA), using a hard tick, Haemaphysalis longicornis, from China for comparative purposes. The ITS rDNA was amplified by polymerase chain reaction (PCR) and sequenced from individual ticks. The lengths of the ITS-1 sequences were 238-1819 bp, and the lengths of ITS-2 were 137-1695 bp, respectively, for all ticks sequenced. While sequence variation within a hard tick species was 0-1.5%, nucleotide differences between hard tick species ranged 2-25.2%, indicating that ITS rDNA sequences provide genetic markers for the differentiation of hard ticks from Romania. Hence, a PCR-linked restriction fragment length polymorphism approach was developed for their unequivocal differentiation based on ITS-1 rDNA. This is the first characterization of ticks from Romania using a genetic approach, which provides the foundation for further studies on ticks in Romania and has implications for studying the population genetic structure of the Romanian ticks and for identification and differentiation of closely related ticks. PMID:19462182

  10. Genetic relatedness of genus Oryza from Eastern Himalayan region as revealed by chloroplast matK gene

    Directory of Open Access Journals (Sweden)

    Doris Zodinpuii

    2013-12-01

    Full Text Available Phylogenetic relationship was studied in wild and cultivated rice using the chloroplast matK gene. The aligned sequence fragments were 826bp in length with 7.02% variable and 4.47% phylogenetically informative sites and the estimated Transition/Transversion bias (R was 1.97. Seven hundred and two characters were constant, 74 variable characters were parsimony-uninformative and 50 were parsimony–informative. Haplotypes of Mizoram rice and wild relatives (A genome were more similar than those of distantly related species (B, C/CD, E and G genomes. It further revealed that the EE genome species is most closely related to the CC genome and CCDD genomes. The BBCC genome species had different origins, and their maternal parents had either the BB or CC genome. An additional genome type, HHKK was recognized in O. coarctata and O. schlechteri. Within the AA genome the African, O. glaberrima and O. longistaminatea and American, O. glumipatula and O. barthii were closer to the Indian Oryza species, O. nivara and O. rufipogon. The unknown genome O. malampuzhaensis from India is closer to BB and BBCC genome containing respectively O. punctata from Cameroon and O. minuta from Philippines. CpG rich matK sequences were rich in GG and FF genotypes, whereas CpA rich sequences belonged to BB and BBCC related genomes variety.

  11. Current Status of Tick Fauna in North of Iran

    Directory of Open Access Journals (Sweden)

    P Shayan

    2007-04-01

    Full Text Available Background: The distribution and ecological preferences of ticks of domestic animals in North of Iran were studied four times a year from 2002 to 2005. Methods: A total of 1720 tick specimens were collected from cattle, sheep and goats from different localities of Caspian Sea areas consisting of Guilan, Mazandaran, Golestan and Ardebil provinces, Iran. Results: Fourteen tick species were identified as Hyalomma anatolicum anatolicum (5.23%, H.marginatum (20.34%, H.detritum (3.48%, Haemaphysalis punctata (12.79%, Haem. Parva (0.58%, Haem.concinna (0.58%, Haem.choldokovsky (6.97%, Ixodes ricinus (2.32%, Rhipicephalus sanguineus (19.76%, Rh.bursa (4.65%, Boophilus annulatus (9.88%, Dermacentor niveus (6.39%, D. marginatus (1.74% and Ornithodoros lahorensis (5.23%. Both Dermacentor and Ornithodoros were found only in Ardebil with cold climatic conditions and high altitude. The only ticks, which were found in forest area, were Boophilus annulatus and Ixodes ricinus. Conclusion: The veterinary and public health importance of the above species should be emphasized.

  12. The parasitic fauna of the European bison (Bison bonasus) (Linnaeus, 1758) and their impact on the conservation. Part 1. The summarising list of parasites noted.

    Science.gov (United States)

    Karbowiak, Grzegorz; Demiaszkiewicz, Aleksander W; Pyziel, Anna M; Wita, Irena; Moskwa, Bożena; Werszko, Joanna; Bień, Justyna; Goździk, Katarzyna; Lachowicz, Jacek; Cabaj, Władysław

    2014-09-01

    During the current century, 88 species of parasites have been recorded in Bison bonasus. These are 22 species of protozoa (Trypanosoma wrublewskii, T. theileri, Giardia sp., Sarcocystis cruzi, S. hirsuta, S. hominis, S. fusiformis, Neospora caninum, Toxoplasma gondii, Cryptosporidium sp., Eimeria cylindrica, E. subspherica, E. bovis, E. zuernii, E. canadensis, E. ellipsoidalis, E. alabamensis, E. bukidnonensis, E. auburnensis, E. pellita, E. brasiliensis, Babesia divergens), 4 trematodes species (Dicrocoelium dendriticum, Fasciola hepatica, Parafasciolopsis fasciolaemorpha, Paramphistomum cervi), 4 cestodes species (Taenia hydatigena larvae, Moniezia benedeni, M. expansa, Moniezia sp.), 43 nematodes species (Bunostomum trigonocephalum, B. phlebotomum, Chabertia ovina, Oesophagostomum radiatum, O. venulosum, Dictyocaulus filaria, D.viviparus, Nematodirella alcidis, Nematodirus europaeus, N. helvetianus, N. roscidus, N. filicollis, N. spathiger, Cooperia oncophora, C. pectinata, C. punctata, C. surnabada, Haemonchus contortus, Mazamastrongylus dagestanicus, Ostertagia lyrata, O. ostertagi, O. antipini, O. leptospicularis, O. kolchida, O. circumcincta, O. trifurcata, Spiculopteragia boehmi, S. mathevossiani, S. asymmetrica, Trichostrongylus axei, T. askivali, T. capricola, T. vitrinus, Ashworthius sidemi, Onchocerca lienalis, O. gutturosa, Setaria labiatopapillosa, Gongylonema pulchrum, Thelazia gulosa, T. skrjabini, T. rhodesi, Aonchotheca bilobata, Trichuris ovis), 7 mites (Demodex bisonianus, D. bovis, Demodex sp., Chorioptes bovis, Psoroptes equi, P. ovis, Sarcoptes scabiei), 4 Ixodidae ticks (Ixodes ricinus, I. persulcatus, I. hexagonus, Dermacentor reticulatus), 1 Mallophaga species (Bisonicola sedecimdecembrii), 1 Anoplura (Haematopinus eurysternus), and 2 Hippoboscidae flies (Lipoptena cervi, Melophagus ovinus). There are few monoxenous parasites, many typical for cattle and many newly acquired from Cervidae. PMID:25119348

  13. Application of a continuously stirred tank bioreactor (CSTR) for bioremediation of hydrocarbon-rich industrial wastewater effluents

    International Nuclear Information System (INIS)

    A continuously stirred tank bioreactor (CSTR) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. A successful bioremediation was developed by an efficient acclimatized microbial consortium. After an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. The performance of the bioaugmented reactor was demonstrated by the reduction of COD rates up to 95%. The residual total petroleum hydrocarbon (TPH) decreased from 320 mg TPH l-1 to 8 mg TPH l-1. Analysis using gas chromatography-mass spectrometry (GC-MS) identified 26 hydrocarbons. The use of the mixed cultures demonstrated high degradation performance for hydrocarbons range n-alkanes (C10-C35). Six microbial isolates from the CSTR were characterized and species identification was confirmed by sequencing the 16S rRNA genes. The partial 16S rRNA gene sequences demonstrated that 5 strains were closely related to Aeromonas punctata (Aeromonas caviae), Bacillus cereus, Ochrobactrum intermedium, Stenotrophomonas maltophilia and Rhodococcus sp. The 6th isolate was affiliated to genera Achromobacter. Besides, the treated wastewater could be considered as non toxic according to the phytotoxicity test since the germination index of Lepidium sativum ranged between 57 and 95%. The treatment provided satisfactory results and presents a feasible technology for the treatment of hydrocarbon-rich wastewater from petrochemical industries and petroleum refineries.

  14. Cargols terrestres (Gastropoda, Stylommatophora del Parc Natural de la Muntanya de Montserrat (Barcelona, NE península ibèrica

    Directory of Open Access Journals (Sweden)

    Bros, V.

    2006-11-01

    Full Text Available Land snails (Gastropoda, Stylommatophora in the Natural Park of Montserrat (Barcelona, NE Iberian peninsula The inventory of 73 land snails in the Natural Park of Montserrat was updated following the review of 130 publications. Planned field study was also conducted in areas of different habitats on the Montserrat mountain to provide a preliminary description of the communities of land snails in the study area. A total of 342 samples of land snails were studied and 50 species were identified. The most frequent were Pomatias elegans, Helicigona lapicida, Pseudotachea splendida, Abida polyodon and Otala punctata. In this region of the prelittoral Catalan mountain range, the level of endemism was high for Abida secale bofilli, Montserratina bofilliana and Xerocrassa montserratensis.The results of the field work extend the faunistic catalogue of the Natural Park of Montserrat to include references to Hygromia cinctella, Microxeromagna lowei, Paralaoma servilis and Punctum pygmaeum in the area. Finally, investigation and conservation programmes are suggested for the endemic species Xerocrassa montserratensis, protected by the Plan for Areas of Natural Interest (PEIN approved by Decree 328/1992.

  15. Presence of host-seeking Ixodes ricinus and their infection with Borrelia burgdorferi sensu lato in the Northern Apennines, Italy.

    Science.gov (United States)

    Ragagli, Charlotte; Mannelli, Alessandro; Ambrogi, Cecilia; Bisanzio, Donal; Ceballos, Leonardo A; Grego, Elena; Martello, Elisa; Selmi, Marco; Tomassone, Laura

    2016-06-01

    Host-seeking ticks were collected in the Northern Apennines, Italy, by dragging at 35 sites, at altitudes ranging from 680 and 1670 m above sea level (asl), from April to November, in 2010 and 2011. Ixodes ricinus (4431 larvae, 597 nymphs and 12 adults) and Haemaphysalis punctata (11,209 larvae, 313 nymphs, and 25 adults) were the most abundant species, followed by Haemaphysalis sulcata (20 larvae, five nymphs, and 13 adults), Dermacentor marginatus (42 larvae and two adults) and Ixodes hexagonus (one nymph). Greatest numbers of ticks were collected at locations characterised by southern exposure and limestone substratum, at altitudes Borrelia burgdorferi sensu lato (sl) in 294 host-seeking I. ricinus nymphs was 8.5 %. Borrelia garinii was the most frequently identified genospecies (64.0 % of positive nymphs), followed by B. valaisiana, B. burgdorferi sensu stricto, B. afzelii, and B. lusitaniae. Based upon the comparison with the results of previous studies at the same location, these research findings suggest the recent invasion of the study area by the tick vector and the agents of Lyme borreliosis. PMID:26964552

  16. Molecular survey of tick-borne pathogens in Ixodid ticks collected from hunted wild animals in Tuscany, Italy

    Institute of Scientific and Technical Information of China (English)

    Valentina; Virginia; Ebani; Fabrizio; Bertelloni; Barbara; Turchi; Dario; Filogari; Domenico; Cerri

    2015-01-01

    Objective: To determine the prevalence of zoonotic tick-borne bacteria in feeding ticks removed from hunted wild animals. Methods: PCR was executed on DNA extracted from 77 tick pools to detect Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi sensu lato, Coxiella burnetii and Rickettsia spp. Results: A total of 432 ticks were collected: 30(6.94%) Haemaphysalis punctata, 72(16.7%) Dermacentor marginatus and 330(76.38%) Ixodes ricinus. For each animal one or two pools of 3 ticks of the same species was constituted. Seventy-seven tick pools were examined by PCR: 58(75.32%) resulted infected and among them 14(18.18%) showed co-infections. In particular, 29(37.66%) pools were positive for Bartonella spp., 23(29.87%) for Anaplasma phagocytophilum, 16(20.78%) for Rickettsia spp., and 5(6.49%) for Borrelia burgdorferi s.l. All samples were negative for Coxiella burnetii. Conclusions: The results demonstrate the presence of several zoonotic tick-borne pathogens in the studied area, and underline the risk of exposure to infections for hunters not only during the outdoor activity, but also when they manipulate hunted animals infested by infected ticks.

  17. Comparative research on protein production capacity and freeze resistance capability of duckweed in nitrogen-phosphorus wastewater purification%浮萍净化氮磷污水的蛋白质生产能力及耐寒能力比较研究

    Institute of Scientific and Technical Information of China (English)

    沈根祥; 黄丽华; 钱晓雍; 徐介乐; 吴健; 唐浩

    2006-01-01

    以3种国外优化浮萍为参照,通过对比试验,对5种长江三角洲地区本地浮萍在净化氮磷污水过程中的蛋白质生产能力及耐寒能力进行了比较研究.结果表明:本地少根紫萍和3种国外浮萍,无论在蛋白质生产能力上,还是在浮萍植物体(干基)蛋白质含量上,均具有相当高的水平.2种本地浮萍(稀脉浮萍和少根紫萍)和2种国外优质浮萍(Lemna minor 8627和Spirodella punctata 7776)能在低温条件下成活生长,具有良好的耐寒能力.

  18. Application of a continuously stirred tank bioreactor (CSTR) for bioremediation of hydrocarbon-rich industrial wastewater effluents

    Energy Technology Data Exchange (ETDEWEB)

    Gargouri, Boutheina; Karray, Fatma; Mhiri, Najla; Aloui, Fathi [Laboratoire des Bioprocedes Environnementaux, Pole d' Excellence Regional AUF-LBPE, Centre de Biotechnologie de Sfax, Universite de Sfax, BP 1117, 3018 Sfax (Tunisia); Sayadi, Sami, E-mail: sami.sayadi@cbs.rnrt.tn [Laboratoire des Bioprocedes Environnementaux, Pole d' Excellence Regional AUF-LBPE, Centre de Biotechnologie de Sfax, Universite de Sfax, BP 1117, 3018 Sfax (Tunisia)

    2011-05-15

    A continuously stirred tank bioreactor (CSTR) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. A successful bioremediation was developed by an efficient acclimatized microbial consortium. After an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. The performance of the bioaugmented reactor was demonstrated by the reduction of COD rates up to 95%. The residual total petroleum hydrocarbon (TPH) decreased from 320 mg TPH l{sup -1} to 8 mg TPH l{sup -1}. Analysis using gas chromatography-mass spectrometry (GC-MS) identified 26 hydrocarbons. The use of the mixed cultures demonstrated high degradation performance for hydrocarbons range n-alkanes (C10-C35). Six microbial isolates from the CSTR were characterized and species identification was confirmed by sequencing the 16S rRNA genes. The partial 16S rRNA gene sequences demonstrated that 5 strains were closely related to Aeromonas punctata (Aeromonas caviae), Bacillus cereus, Ochrobactrum intermedium, Stenotrophomonas maltophilia and Rhodococcus sp. The 6th isolate was affiliated to genera Achromobacter. Besides, the treated wastewater could be considered as non toxic according to the phytotoxicity test since the germination index of Lepidium sativum ranged between 57 and 95%. The treatment provided satisfactory results and presents a feasible technology for the treatment of hydrocarbon-rich wastewater from petrochemical industries and petroleum refineries.

  19. Impact of tannery effluents on the aquatic environment of the Buriganga River in Dhaka, Bangladesh.

    Science.gov (United States)

    Asaduzzaman, Mohammad; Hasan, Imtiaj; Rajia, Sultana; Khan, Nazneen; Kabir, Kazi Ahmed

    2016-06-01

    This study presents an overview of the existence and effects of six heavy metals, chromium (Cr), lead (Pb), cadmium (Cd), mercury (Hg), manganese (Mn), and aluminum (Al), in tannery effluents released to the Buriganga River in Dhaka, Bangladesh. The pollutants were found in three different sources, such as effluents from tanneries, contaminated river water and three species of fish-climbing perch (Anabas testudineus), spotted snakehead (Channa punctata), and Black tilapia (Oreochromis mossambicus) caught from the river. Tannery effluents, water, and fish samples were collected from three different factories, five sample stations, and three different harvesting points, respectively. Effluents from all three factories contained significant amounts of heavy metals, especially Cr (374.19 ppm in average), whereas lesser amounts were found in the tissues of the three fish species studied. The trends in tissue elemental concentrations of fish were Cr > Pb > Al > Hg > Mn > Cd. In most cases (Cr, Cd, Mn, and Al), heavy metal concentrations were found to be greater in climbing perch than in Black tilapia and spotted snakehead. Although the river water contained high concentrations of harmful heavy metals, the fish species under study had concentrations well below the permissible Food and Agriculture Organization/World Health Organization levels for those metals and seemed to be safe for human consumption. PMID:25216801

  20. Interspecific variation of total seed protein in wild rice germplasm using SDS-Page

    International Nuclear Information System (INIS)

    Variation in seed protein of 14 wild rice species (Oryza spp.) along with cultivated rice species (O. sativa) was studied using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to assess genetic diversity in the rice germplasm. SDS bands were scored as present (1) or absent (0) for protein sample of each genotype. On the basis of cluster analysis, four clusters were identified at a similarity level of 0.85. O. nivara, O. rufipogon and O. sativa with AA genomes constituted the first cluster. The second cluster comprised O. punctata of BB genome and wild rice species of CC genome i.e., O. rhizomatis and O. officinalis. However, it also contained O. barthii and O. glumaepatula of AA genome. O. australiensis with EE genome, and O. latifolia, O. alta and O. grandiglumis having CCDD genomes comprised the third cluster. The fourth cluster consisted of wild rice species, O. brachyantha with EE genome along with two other wild rice species, O. longistaminata and O. meridionalis of AA genome. Overall, on the basis of total seed protein, the grouping pattern of rice genotypes was mostly compatible with their genome status. The results of the present work depicted considerable interspecific genetic variation in the investigated germplasm for total seed protein. Moreover, the results obtained in this study also suggest that analysis of seed protein can also provide a better understanding of genetic affinity of the germplasm. (author)

  1. Anthelmintic activity of albendazole against gastrointestinal nematodes in calves.

    Science.gov (United States)

    Benz, G W; Ernst, J V

    1977-09-01

    Anthelmintic activities of albendazole were evaluated in a controlled experiment. Forty calves experimentally infected with gastrointestinal nematodes were allotted to 4 groups. Calves in group 1 were used as nonmedicated controls; calves in groups 2, 3, and 4 were given (by oral route) a suspension containing albendazole at dose concentrations of 2.5, 5.0, and 7.5 mg/kg of body weight on the 35th day after administration of infective nematode larvae. In groups 2, 3, and 4 calves, average overall reductions (based on geometric means) were 77.1, 93.6, and 98.1%, respectively. These reductions were highly significant (P less than 0.01) in calves given doses of 5.0 and 7.5 mg/kg, and were significant (P less than 0.05) in calves given the 2.5-mg/kg dose. Ostertagia ostertagi, Trichostrongylus axei, Cooperia onchophora, Cooperia punctata, and Oesophagostomum radiatum removals at the 5.0- and 7.5-mg/kg dose levels were all highly significant (P less than 0.01); whereas, removals of Haemonchus contortus were not significant, even at the 7.5-mg/kg dose level. PMID:921039

  2. Survey of tick species parasiting domestic ruminants in Ghaemshahr county, Mazandaran province, Iran

    Institute of Scientific and Technical Information of China (English)

    Hosseini Vasoukolaei Nasibeh; Telmadarraiy Zakkyeh; Vatandoost Hassan; Yaghoobi Ershadi Mohammad Reza; Hosseini Vasoukolaei Morteza; Oshaghi Mohammad Ali

    2010-01-01

    Objective:To determine the tick species parasitizing domestic ruminants in Ghaemshahr county in Mazandaran, a Caspian province in the north of Iran.Methods:About361 sheep, 54 goats and10cattle of18 herds in several villages in Ghaemshahr were inspected for tick infestation. Separated ticks were preserved in70% alcohol and identified.Results:About323 ticks (207female,116 male) were collected, the occurrence of ticks on sheep, goats and cattle were28.3%, 22.2% and20.0%respectively. The mean number of ticks on each animal was low (3-5ticks per animal).Rhipicephalus sanguineus, Rhipicephalus bursa, Ixodes ricinus, Boophilus annulatus, Haemaphysalis punctata andHaemaphysalis numidiana were the tick species we found.Rhipicephalus sanguineus were the most abundant species in the study area. The largest number of ticks were generally present from April to July, mostly in animal ears and tails. Ixodes, Boophilus andHaemaphysalis occurred in mountainous areas of Ghaemshahr, whereas Rhipicephalus were present in both mountains and plains of the study area.Conclusions: The result of this study is a survey of tick species from domestic animals in Iran and implication of possible prevention measures for diseases transmitted by ticks.

  3. Anaplasma marginale and Theileria annulata in questing ticks from Portugal.

    Science.gov (United States)

    Antunes, S; Ferrolho, J; Domingues, N; Santos, A S; Santos-Silva, M M; Domingos, A

    2016-09-01

    Ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. Monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. In this study 428 host-seeking ticks, belonging to nine species of Ixodidae and collected from 17 of the 23 Portuguese mainland subregions, were screened for several tick-borne agents with veterinary relevance: Anaplasma marginale, Anaplasma ovis, Anaplasma centrale, Babesia spp., Coxiella burnetii and Theileria spp. Prevalence was assessed by PCR and amplified amplicons sequenced for validation of results. Twenty ticks, in a total of 428, were found positive: one Ixodes ventalloi for Theileria annulata and four Dermacentor marginatus, one Haemaphysalis punctata, five Ixodes ricinus, five I. ventalloi, and four Rhipicephalus sanguineus sensu lato for A. marginale. According to the reviewed literature, this is the first report of A. marginale and T. annulata detection in I. ventalloi. Furthermore, the amplification of A. marginale DNA in several tick species suggests a broad range for this agent in Portugal that might include other uncommon species as R. sanguineus s.l. This work provides new data towards a better understanding of tick-pathogen associations and also contributes to the surveillance of tick-borne agents in geographic areas with limited information. PMID:27394441

  4. Determination of hard tick species (Acarina:Ixodidae) on sheep and cattle in Hamedan Province, Iran

    Institute of Scientific and Technical Information of China (English)

    Jamal Gharekhani; Abbas Gerami-Sadeghian; Zivar Sadeghi-Dehkordi; Mohammadreza Youssefi

    2015-01-01

    Objective: To determine the fauna and frequency of hard tick species on sheep and cattle in Hamedan Province, Western Iran. Methods: Tick sampling was performed on the whole body of 18000 sheep and 4200 cattle in 3 rural regions (mountain, plateau, and plain-mountainous zone) during the year of 2010 to 2011. The ticks were identified with appropriate identification keys. Results: A total of 1534 hard ticks (62.1% male and 37.9% female) were collected in animals. The infestation rate was found 2.4% in animals (4.2% in cattle and 2.0% in sheep). The ticks were classified into 3 genera and 7 species including: Hyalomma marginatum (34.1%), Hyalomma excavatum (29.7%), Rhipicephalus bursa (13.8%), Rhipicephalus sanguineus (7.5%), Hyalomma detritum (7.1%), Haemaphysalis punctata (5.1%) and Hyalomma dromedarii (2.7%). Conclusions: Current study is the first report of fauna and frequency of hard ticks in this region. The results showed that Hyalomma marginatum is the dominant hard tick species. Further studies are needed to determine the importance of Ixodidae ticks of veterinary and public health in this region of Iran.

  5. Additions to the knowledge of the land snails of Sabah (Malaysia, Borneo), including 48 new species

    Science.gov (United States)

    Vermeulen, Jaap J.; Liew, Thor-Seng; Schilthuizen, Menno

    2015-01-01

    Abstract We present reviews of the Sabah (Malaysia, on the island of Borneo) species of the following problematical genera of land snails (Mollusca, Gastropoda): Acmella and Anaglyphula (Caenogastropoda: Assimineidae); Ditropopsis (Caenogastropoda: Cyclophoridae); Microcystina (Pulmonata: Ariophantidae); Philalanka and Thysanota (Pulmonata: Endodontidae); Kaliella, Rahula, (Pulmonata: Euconulidae); Trochomorpha and Geotrochus (Pulmonata: Trochomorphidae). Next to this, we describe new species in previously revised genera, such as Diplommatina (Diplommatinidae); Georissa (Hydrocenidae); as well as some new species of genera not revised previously, such as Japonia (Cyclophoridae); Durgella and Dyakia (Ariophantidae); Amphidromus, and Trachia (Camaenidae); Paralaoma (Punctidae); Curvella (Subulinidae). All descriptions are based on the morphology of the shells. We distinguish the following 48 new species: Acmella cyrtoglyphe, Acmella umbilicata, Acmella ovoidea, Acmella nana, Acmella subcancellata, Acmella striata, and Anaglyphula sauroderma (Assimineidae); Ditropopsis davisoni, Ditropopsis trachychilus, Ditropopsis constricta, Ditropopsis tyloacron, Ditropopsis cincta, and Japonia anceps (Cyclophoridae); Diplommatina bidentata and Diplommatina tylocheilos (Diplommatinidae); Georissa leucococca and Georissa nephrostoma (Hydrocenidae); Durgella densestriata, Dyakia chlorosoma, Microcystina microrhynchus, Microcystina callifera, Microcystina striatula, Microcystina planiuscula, and Microcystina physotrochus (Ariophantidae); Amphidromus psephos and Trachia serpentinitica (Camaenidae); Philalanka tambunanensis, Philalanka obscura, Philalanka anomphala, Philalanka rugulosa, and Philalanka malimgunung (Endodontidae); Kaliella eurytrochus, Kaliella sublaxa, Kaliella phacomorpha, Kaliella punctata, Kaliella microsoma, Rahula delopleura, (Euconulidae); Paralaoma angusta (Punctidae); Curvella hadrotes (Subulinidae); Trochomorpha trachus, Trochomorpha haptoderma, Trochomorpha

  6. Induced-fit Mechanism for Prolyl Endopeptidase

    Energy Technology Data Exchange (ETDEWEB)

    Li, Min; Chen, Changqing; Davies, David R.; Chiu, Thang K. (NIH); (LSU); (Chinese Aca. Sci.)

    2010-11-15

    Prolyl peptidases cleave proteins at proline residues and are of importance for cancer, neurological function, and type II diabetes. Prolyl endopeptidase (PEP) cleaves neuropeptides and is a drug target for neuropsychiatric diseases such as post-traumatic stress disorder, depression, and schizophrenia. Previous structural analyses showing little differences between native and substrate-bound structures have suggested a lock-and-key catalytic mechanism. We now directly demonstrate from seven structures of Aeromonus punctata PEP that the mechanism is instead induced fit: the native enzyme exists in a conformationally flexible opened state with a large interdomain opening between the {beta}-propeller and {alpha}/{beta}-hydrolase domains; addition of substrate to preformed native crystals induces a large scale conformational change into a closed state with induced-fit adjustments of the active site, and inhibition of this conformational change prevents substrate binding. Absolute sequence conservation among 28 orthologs of residues at the active site and critical residues at the interdomain interface indicates that this mechanism is conserved in all PEPs. This finding has immediate implications for the use of conformationally targeted drug design to improve specificity of inhibition against this family of proline-specific serine proteases.

  7. A Preliminary Study on the Chemotaxis of Hemocytes from Freshwater Mussels Stimulated by Bacteria%河蚌血细胞对细菌趋化移动的初步研究

    Institute of Scientific and Technical Information of China (English)

    李静; 石安静; 刘克武; 袁志刚

    2003-01-01

    采用改进的毛细管法,研究了圆背角无齿蚌(Anodonta woodiana pacifica)和三角帆蚌(Hyriopsis cumingii)两种淡水河蚌离体血细胞对两种水体中常见病原细菌的趋化移动作用,及血清对其的影响.结果显示,两种河蚌的离体血细胞对细菌都具有趋化移动作用,产生趋化移动的血细胞数量都显著高于无细菌的对照组(P<0.05).在有血清时,血细胞对荧光极毛杆菌(Pseudomonas fluorescens)的趋化移动活性略高于肠型点状气单孢菌(Aeromonas punctata f.intestinalis),圆背角无齿蚌离体血细胞的趋化移动能力显著高于三角帆蚌(P<0.05).血清对河蚌离体血细胞的趋化移动作用有显著的促进作用(P<0.05).

  8. Evaluation of genetic toxicity caused by acid mine drainage of coal mines on fish fauna of Simsang River, Garohills, Meghalaya, India.

    Science.gov (United States)

    Talukdar, B; Kalita, H K; Baishya, R A; Basumatary, S; Sarma, D

    2016-09-01

    Fishery ecology of the Simsang River, Meghalaya is being threatened by large scale environmental degradation due to acid mine drainage (AMD) of coal mines. In the present paper, effort has been made to evaluate the genotoxicity caused due to AMD of coal mines on Channa punctata under laboratory condition through comet assay, micronucleus and chromosome aberration tests. Water samples were collected seasonally from affected and unaffected sites of the River and physico-chemical quality of water indicated low pH (4.6), high concentration of sulphates (270mgL(-1)) and iron (7.2mgL(-1)) beyond permissible limits. Polycyclic aromatic hydrocarbon (PAH) showed highest concentration of 4-ring PAH and Benzo[a]anthracene was the most important pollutant in the water collected from affected sites. The highest and the lowest mean concentrations of PAHs were estimated in monsoon and winter season, respectively. The index of DNA damage assessed by comet assay, micronucleus and chromosome aberration tests demonstrated significant differences season wise in different sampling sites. Frequency of DNA-damaged cells was found highest in the water samples collected from affected site in monsoon season. PMID:27213561

  9. An annotated and revised checklist of pleurostome ciliates (Protista: Ciliophora: Litostomatea) from Slovakia, Central Europe.

    Science.gov (United States)

    Vďačný, Peter; Rajter, L'ubomír

    2014-01-01

    Pleurostomatids are predatory ciliates, living especially in the periphyton and benthos of various freshwater and marine habitats. In the present work, we provide an annotated and revised checklist of this ciliate group from the territory of Slovakia. Altogether 29 pleurostome species belonging to five genera have been reported there: Acineria incurvata, Ac. punctata, Ac. uncinata, Amphileptus claparedii, Am. falcatus, Am. fusiformis, Am. parafusidens, Am. pleurosigma, Am. procerus, Am. punctatus, A. rotundus, Litonotus alpestris, Li. anguilla, Li. carinatus, Li. crystallinus, Li. cygnus, Li. fasciola, Li. fusidens, Li. hirundo, Li. lamella, Li. minisculus, Li. muscorum, Li. obtusus, Li. triqueter, Li. varsaviensis, Loxophyllum helus, Lo. meleagris, Lo. rostratum, and Siroloxophyllum utriculariae. We have catalogued these records providing the following data for each species: (1) author(s) and date of publication; (2) name(s) of the species as appeared in the publication(s) followed by chronologically listed references including relevant page(s) in literature; (3) nomenclatural and taxonomic notes if needed; (4) main morphological characters; (5) morphological data on Slovak populations if available; and (6) all faunistic records. PMID:24870102

  10. HISTORICAL ECOTOXICOLOGICALASSESSMENT OF SAN JUAN ECOSYSTEM, SANTIAGO DE CUBA, CUBA

    Directory of Open Access Journals (Sweden)

    Argota, George

    2013-07-01

    Full Text Available Currently, the environmental decision-making related to watershed preservation is oriented under the precepts of an historical environmental assessment. The objective of this research was to conduct a historical ecotoxicological assessment of the San Juan de Santiago de Cuba ecosystem. This was considered a systematic environmental monitoring which ran from 2010 to 2013, made q?uarterly. Physicochemical parameters such as electrical conductivity, total alkalinity, total hardness, pH, total solids, dissolved oxygen, biochemical oxygen demand and chemical oxygen demand were determined. All parameters of environmental risk prediction were performed using the GECOTOX program. The concentrations of Cu, Zn, Pb and Cd on three target organs (brain, gills and liver in the biomonitor adult Gambusia punctata (Poecilidae were analyzed. In this species, the effect of total protein and enzymatic activity of acetylcholinesterase as biomarkers were evaluated. In general, the parameters were outside the values set used by the Cuban quality standards. The GECOTOX program said that the waters of the San Juan ecosystem presented a high rate of risk. The highest concentrations of metals were determined in the gills, liver and brain, respectively. Biomarker values w? ere high, being higher for females. Finally, it was concluded that from one year to another all responses were ascending; thus, the San Juan ecosystem presented ecotoxicological effects in time.

  11. Taxonomic and Functional Resilience of Grasshoppers (Orthoptera, Caelifera) to Fire in South Brazilian Grasslands.

    Science.gov (United States)

    Ferrando, C P R; Podgaiski, L R; Costa, M K M; Mendonça, M D S

    2016-08-01

    Fire is a frequent disturbance in grassland ecosystems enabling variability in habitat characteristics and creating important environmental filters for community assembly. Changes in vegetation have a large influence on herbivore insect assemblages. Here, we explored the responses of grasshoppers to disturbance by fire in grasslands of southern Brazil through a small-scale experiment based in paired control and burned plots. The resilience of grasshoppers was assessed by monitoring changes to their abundance, taxonomic, and functional parameters along time. Burned patches have been already recolonized by grasshoppers 1 month after fire and did not differ in terms of abundance and richness from control areas in any evaluated time within 1 year. Simpson diversity decreased 1 month after fire due to the increased dominance of Dichroplus misionensis (Carbonell) and Orphulella punctata (De Geer). In this period, grasshoppers presented in average a smaller body and a larger relative head size; these are typically nymph characteristics, which are possibly indicating a preference of juveniles for the young high-quality vegetation, or a diminished vulnerability to predation in open areas. Further, at 6 months after fire grasshoppers with smaller relative hind femur and thus lower dispersal ability seemed to be benefitted in burned patches. Finally, 1 year after fire grasshoppers became more similar to each other in relation to their set of traits. This study demonstrates how taxonomic and functional aspects of grasshopper assemblages can be complementary tools to understand their responses to environmental change. PMID:26957086

  12. Effects of edaphic factors on the tree stand diversity in a tropical forest of Sierra Madre del Sur, Mexico

    Science.gov (United States)

    Kurzmeier, S.; Wiedemann, T.; Biber, P.; Schad, P.; Krasilnikov, P. V.

    2012-08-01

    Two sites with similar environmental parameters, except for the edaphic factor, were selected in the mountainous tropical forest of southern Mexico. Site 1 was established on an Alisol; site 2, on a Phaeozem. Representative soil profiles were examined on each of the sites, and topsoil was sampled on a regular grid pattern. The soil of site 2 was richer in organic matter and major nutrients and had a less acid reaction than the soil of site 1. The species diversity of the trees at site 2 (30 species) was higher than that at site 1 (17 species). The species compositions of the trees were different on the two soils: there were only six species in common for both sites. The coefficients of species similarity on the sites were low. We concluded that the presence of different soils within the same type of forest ecosystem increases its β-diversity. The examination of edaphic preferences of the species showed that Alstonia longifolia and Thouinidium decandrum preferred rich soils, Inga punctata and Ocotea sinuata preferred poor soils, and Cupania dentata and Hamelia patens did not display preferences in the studied range of soil properties. Thus, the spatial variability of the soil properties affect the spatial pattern of tree species in the studied tropical forest ecosystems.

  13. New ultrastructural aspects of the spermatozoon of Aplysia depilans (Gastropoda, Opisthobranchia).

    Science.gov (United States)

    Vita, P; Corral, L; Azevedo, C

    2001-01-01

    The spermatozoon of the sea hare Aplysia depilans was studied under scanning (SEM) and transmission electron microscopy (TEM). Previous descriptions of this sperm and related species, both from light and electron microscopy, were inconsistent with each other. These descriptions include A. depilans, A. punctata, A. fasciata, A. kurodai and Bursatella leachiplei. Several detailed micrographs provide a new ultrastructural model and reveal new aspects such as the presence of acrosome and the absence of a glycogen piece, therefore the modified dense ring is the terminal structure. Results also show that previous models are incorrect in many aspects. The spermatozoon is a long slender uniflagellated cell with a complex helical structure and a length of approximately 165 microm. Observed in SEM the spermatozoon has an undifferentiated head and tail. The nucleus is cord-shaped and helically intertwined with the axoneme/mitochondrial derivative complex. The mitochondrial derivative has only one glycogen helix. Glycogen presence was demonstrated by Thiéry's method. Typical heterobranchia spermatozoa features are recognised. From bibliographic analysis, a high degree of similarity was found with the sperm of Pleurobranchea maculata (Notaspidea). PMID:11686398

  14. Functional neuroanatomy of the rhinophore of Archidoris pseudoargus

    Science.gov (United States)

    Wertz, Adrian; Rössler, Wolfgang; Obermayer, Malu; Bickmeyer, Ulf

    2007-06-01

    For sea slugs, chemosensory information represents an important sensory modality, because optical and acoustical information are limited. In the present study, we focussed on the neuroanatomy of the rhinophores and processing of olfactory stimuli in the rhinophore ganglion of Archidoris pseudoargus, belonging to the order of Nudibranchia in the subclass of Opisthobranchia. Histological techniques, fluorescent markers, and immunohistochemistry were used to analyse neuroanatomical features of the rhinophore. A large ganglion and a prominent central lymphatic channel are surrounded by longitudinal muscles. Many serotonin-immunoreactive (IR) processes were found around the centre and between the ganglion and the highly folded lobes of the rhinophore, but serotonin-IR cell bodies were absent inside the rhinophore. In contrast to the conditions recently found in Aplysia punctata, we found no evidence for the presence of olfactory glomeruli within the rhinophore. Using calcium-imaging techniques with Fura II as a calcium indicator, we found differential calcium responses in various regions within the ganglion to stimulation of the rhinophore with different amino acids. The lack of glomeruli in the rhinophores induces functional questions about processing of chemical information in the rhinophore.

  15. Rare and Endangered Geophyte Plant Species in Serpentine of Kosovo

    Directory of Open Access Journals (Sweden)

    Naim Berisha

    2014-12-01

    Full Text Available Our study documents information on rarity, geographical distribution, taxonomy and conservation status of 11 geophyte species in serpentine soils of Kosovo, already included in the Red Book of Vascular Flora of Kosovo. Kosovo’s serpentine vegetation represents a diversity that yet has not been sufficiently explored. Large serpentine complexes are found in the northern Kosovo but also southern part of the country is rich in serpentines, therefore in endemics. Serpentine rocks and soils are characterized by low level of principal plant nutrients (N, P, K, Ca and exceptionally high levels of Mg and Fe. Serpentines play particular importance for flora of the country due to their richness in endemic plant species. The following 11 plant species have been studied: Aristolochia merxmuelleri, Colchicum hungaricum, Crocus flavus, Crocus kosaninii, Epimedium alpinum, Gentiana punctata, Gladiolus illyricus, Lilium albanicum, Paeonia peregrina, Tulipa gesneriana and Tulipa kosovarica. Five out of eleven studied geophytes fall within Critically Endangered IUCN based threat category and five out of eleven are local endemics. Aristolochia merxmuelleri and Tulipa kosovarica are steno-endemic plant species that are found exclusively in serpentine soils. Information in our database should prove to be valuable to efforts in ecology, floristics, biosystematics, conservation and land management.

  16. Gastro-intestinal nematodes and cestodes of cattle in Burkina Faso

    Directory of Open Access Journals (Sweden)

    Belem A.M.G.

    2001-01-01

    Full Text Available A survey of the parasites of abomasa, small, and large intestines of 94 bovines conveyed to the main slaughterhouse of Ouagadougou from the central and northern part of Burkina Faso allowed the identification of nine different worm species: Cooperia punctata, Cooperia pectinata, Haemonchus contortus, Trichostrongylus colubriformis, Bunostomum phlebotomum, Moniezia expensa, Avitellina sp., Oesophagostomum radiatum, and Trichuris sp. By far, Cooperia sp. was the most prevalent (89.4/, followed by H. contortus (66/, and O. radiatum (42.6/. The other worm species were much less prevalent. While only one cattle was free of parasites, it was noticed that polyparasitism was very common. There was a wide range of worm burden (0 to 42290 with however in most animals high worm numbers specially of Cooperia sp. This study confirmed that the rainy season constitutes a period of worm explosion. During the hot and dry season, H. contortus seemed primarily able to undergo arrested development in the L4 stage and secondarily to remain in the adult stage. Statistical analyses of levels of infestation did not show any significant difference according to age, sex, and weight of cattle.

  17. Hallazgos oftalmológicos en pacientes con VIH/SIDA en la era pre TARGA

    Directory of Open Access Journals (Sweden)

    Bety Yañez

    2007-07-01

    Full Text Available Se realizó un estudio prospectivo en 821 pacientes con diagnóstico de VIH-SIDA evaluados en la consulta externa de los servicios de Medicina Tropical y Oftalmología del Hospital Nacional Dos de Mayo (Lima, Perú como parte de la evaluación inicial para ingresar al protocolo de TARGA, entre abril de 2004 a julio de 2006. El rango de edad fue de 16- 73 años (media 36, 74% fueron varones, 114(13,9% habían recibido antirretrovirales. Más de la mitad (62% refirieron síntomas oculares, el más frecuente fue la disminución de la agudeza visual (38,4%. Se encontraron lesiones en el segmento anterior en 21,7%, queratopatía punctata superficial, microangiopatía conjuntival, conjuntivitis y cataratas fueron las más frecuentes. Se evidenciaron lesiones en el segmento posterior en 11,9% de los casos, microangiopatía vascular en 47 (5,7% y retinitis por citomegalovirus en 31 (3,8%, que fue la principal causa de ceguera en este grupo de pacientes (14/19 ojos.

  18. Salt glands in flowering culms of Eriochloa species (Poaceae

    Directory of Open Access Journals (Sweden)

    M. O. Arriaga

    1992-12-01

    Full Text Available Salt glands were found in Eriochloa (Paniceae-Poaceae:  E. monte\\idensis, E. pseudoacrotricha and E. punctata.  They occur on the culms, rachises and secondary ramifications of the inflorescence. The glands are bicellular structures with endodermal tissue at the base. They consist of a basal cell and an apical cell, which is a collecting chamber with a large pore at the top. It is proposed to conserve the term salt gland to designate excretory structures associated with endodermal collecting tissue. The elements present in the glands (detected by the use of X-ray micro-analysis are: Na. Mg. P. S. Cl. K with an increase of the elements from the endodermal tissue to the cap cell. Because of energy needed to transport and excrete salts, salt glands are situated at the base of the inflorescence, which is the zone of maximal development of Kranz structure. It is inferred that  Eriochloa is a facultative halophytic genus, derived recently from a halophytic ancestor.

  19. Localization of canine brachycephaly using an across breed mapping approach.

    Directory of Open Access Journals (Sweden)

    Danika Bannasch

    Full Text Available The domestic dog, Canis familiaris, exhibits profound phenotypic diversity and is an ideal model organism for the genetic dissection of simple and complex traits. However, some of the most interesting phenotypes are fixed in particular breeds and are therefore less tractable to genetic analysis using classical segregation-based mapping approaches. We implemented an across breed mapping approach using a moderately dense SNP array, a low number of animals and breeds carefully selected for the phenotypes of interest to identify genetic variants responsible for breed-defining characteristics. Using a modest number of affected (10-30 and control (20-60 samples from multiple breeds, the correct chromosomal assignment was identified in a proof of concept experiment using three previously defined loci; hyperuricosuria, white spotting and chondrodysplasia. Genome-wide association was performed in a similar manner for one of the most striking morphological traits in dogs: brachycephalic head type. Although candidate gene approaches based on comparable phenotypes in mice and humans have been utilized for this trait, the causative gene has remained elusive using this method. Samples from nine affected breeds and thirteen control breeds identified strong genome-wide associations for brachycephalic head type on Cfa 1. Two independent datasets identified the same genomic region. Levels of relative heterozygosity in the associated region indicate that it has been subjected to a selective sweep, consistent with it being a breed defining morphological characteristic. Genotyping additional dogs in the region confirmed the association. To date, the genetic structure of dog breeds has primarily been exploited for genome wide association for segregating traits. These results demonstrate that non-segregating traits under strong selection are equally tractable to genetic analysis using small sample numbers.

  20. Advances in treatment of achondroplasia and osteoarthritis.

    Science.gov (United States)

    Klag, Kendra A; Horton, William A

    2016-04-15

    Achondroplasia (ACH) is the prototype and most common of the human chondrodysplasias. It results from gain-of-function mutations that exaggerate the signal output of the fibroblast growth factor receptor 3 (FGFR3), a receptor tyrosine kinase that negatively regulates growth plate activity and linear bone growth. Several approaches to reduce FGFR3 signaling by blocking receptor activation or inhibiting downstream signals have been proposed. Five show promise in preclinical mouse studies. Two candidate therapies target the extracellular domain of FGFR3. The first is a decoy receptor that competes for activating ligands. The second is a synthetic blocking peptide that prevents ligands from binding and activating FGFR3. Two established drugs, statins and meclozine, improve growth of ACH mice. The strongest candidate therapy employs an analog of C-type natriuretic peptide (CNP), which antagonizes the mitogen-activated-protein (MAP) kinase pathway downstream of the FGFR3 receptor and may also act independently in the growth plate. Only the CNP analog has reached clinical trials. Preliminary results of Phase 2 studies show a substantial increase in growth rate of ACH children after six months of therapy with no serious adverse effects. A challenge for drug therapy in ACH is targeting agents to the avascular growth plate. The application of gene therapy in osteoarthritis offers insights because it faces similar technical obstacles. Major advances in gene therapy include the emergence of recombinant adeno-associated virus as the vector of choice, capsid engineering to target vectors to specific tissues, and development of methods to direct vectors to articular chondrocytes. PMID:26443596

  1. Dexamethasone stimulates expression of C-type Natriuretic Peptide in chondrocytes

    Directory of Open Access Journals (Sweden)

    Beier Frank

    2006-11-01

    Full Text Available Abstract Background Growth of endochondral bones is regulated through the activity of cartilaginous growth plates. Disruption of the physiological patterns of chondrocyte proliferation and differentiation – such as in endocrine disorders or in many different genetic diseases (e.g. chondrodysplasias – generally results in dwarfism and skeletal defects. For example, glucocorticoid administration in children inhibits endochondral bone growth, but the molecular targets of these hormones in chondrocytes remain largely unknown. In contrast, recent studies have shown that C-type Natriuretic Peptide (CNP is an important anabolic regulator of cartilage growth, and loss-of-function mutations in the human CNP receptor gene cause dwarfism. We asked whether glucocorticoids could exert their activities by interfering with the expression of CNP or its downstream signaling components. Methods Primary mouse chondrocytes in monolayer where incubated with the synthetic glucocorticoid Dexamethasone (DEX for 12 to 72 hours. Cell numbers were determined by counting, and real-time PCR was performed to examine regulation of genes in the CNP signaling pathway by DEX. Results We show that DEX does influence expression of key genes in the CNP pathway. Most importantly, DEX significantly increases RNA expression of the gene encoding CNP itself (Nppc. In addition, DEX stimulates expression of Prkg2 (encoding cGMP-dependent protein kinase II and Npr3 (natriuretic peptide decoy receptor genes. Conversely, DEX was found to down-regulate the expression of the gene encoding its receptor, Nr3c1 (glucocorticoid receptor, as well as the Npr2 gene (encoding the CNP receptor. Conclusion Our data suggest that the growth-suppressive activities of DEX are not due to blockade of CNP signaling. This study reveals a novel, unanticipated relationship between glucocorticoid and CNP signaling and provides the first evidence that CNP expression in chondrocytes is regulated by endocrine

  2. Substitution of aspartate for glycine 103 of the type II collagen triple helical domain: Identification of the minimal mutation which can produce Kniest dysplasia

    Energy Technology Data Exchange (ETDEWEB)

    Wilkin, D.J.; Rimoin, D.L.; Cohn, D.H. [Cedars-Sinai Medical Center, Los Angeles, CA (United States)] [and others

    1994-09-01

    Kniest dysplasia is an autosomal dominant chondrodysplasia which results from mutations in the gene for type II collagen, COL2A1. Characteristics of the disorder include a short trunk and extremities, mid-face hypoplasia, cleft palate, myopia, retinal detachment, and hearing loss. Recently, deletions of all or part of exon 12 have been identified in individuals with Kniest dysplasia, suggesting that mutations within this region of the protein may primarily result in the Kniest dysplasia phenotype. We used SSCP to analyze an amplified genomic DNA fragment containing exon 12 from 7 individuals with Kniest dysplasia. An abnormality was identified in one patient. DNA sequence analysis demonstrated that the patient was heterozygous for a G to A transition that implied substitution of glycine{sup 103} of the triple helix by aspartate. The mutation was not observed in DNA from either of the proband`s parents. Protein microsequencing demonstrated expression of the abnormal allele in the proband`s cartilage, indicating that the Kniest phenotype results from the presence of abnormal type II collagen molecules in the extracellular matrix. These data demonstrate the minimal mutation which can produce Kniest dysplasia and further support the hypothesis that alteration of a domain which includes the region encoded by exon 12 in the type II collagen protein leads to this disorder. Experiments designed to identify specific effects that mutations in this region have on intermolecular interactions among abnormal type II collagen molecules and other components of the cartilage extracellular matrix may clarify the underlying pathophysiology of Kniest dysplasia.

  3. Resultados paleoceanográficos deducidos a partir del contenido en diatomeas de los sedimentos siliceos miocenos («moronitas» en la Cuenca del Guadalquivir

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    López García, Mª J.

    1995-04-01

    Full Text Available During a part of the Miocene (Langhian middle-middle Tortonian diatom bearing sediments, named «moronitas», were deposited in the Guadalquivir Basin. This sedimentation was the result of a highly productive coastal upwelling regime in the area. At this time, the oceanic circulation in the «nord-betic strait» was under stuarine conditions and the nutrients carne from deep Atlantic currents. The intensity of the circulation of deep water in oceans is usually related to global climatic changes. Coastal upwelling is not an effective process to bring up nutrient-rich deep waters to the surface. A complex hidrological process must occur to bring up deep water firstly to intermediate leveIs and then the nutrients may be upwelled from subsurface waters. It is necessary to take into account local factors related to the basin morphology and the changes that it has gone through in arder to place the ancient upwelling centers in the basin. The percentajes of the most common species (Crucidenticula nicobarica + Crucidenticula punctata, Denticulopsis hustedtii, Thalassionema nitzschioides, Thalassionema nitzschioides var. parva, Thalassiothrix longissima + Thalassionema bacillaris and the biosilicic global content of the rocks has made possible the recognition of a change in the temperature of superficial waters and upwelling intensity in time. The interpretation of the A..P.C. graphics gives a paleoecological significance to the A.P.C. first two factors. In this way different productivity situations in response to different locations and intensity of the ancient upwelling centers in time have been identified.Durante una parte del Mioceno (Langhiense medio-Tortoniense medio tuvo lugar en actual Cuenca del Guadalquivir la formación de sedimentos con diatomeas («moronitas». Esta sedimentación fue consecuencia de la formación de un área de alta productividad biogénica ocasionada por corrientes ascensionales costeras (coastal upwelling. La

  4. Prevalência de lesões cutâneas actínicas em pacientes com carcinoma basocelular do segmento cefálico: um estudo caso-controle Prevalence of actinic skin lesions in patients with basal cell carcinoma of the head: a case-control study

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    Valquíria Pessoa Chinem

    2012-04-01

    Full Text Available OBJETIVO: Avaliar a preval��ncia de lesões cutâneas actínicas em portadores de carcinoma basocelular do segmento cefálico. MÉTODOS: Foi conduzido estudo tipo caso-controle. Os casos, constituídos por pacientes com carcinoma basocelular sólido, primário, menor que dois centímetros, no segmento cefálico; e controles, por pacientes com outras dermatoses. Foram analisadas variáveis constitucionais, comportamentais e lesões actínicas. RESULTADOS: Avaliaram-se 120 casos e 360 controles. Mílio facial (OR = 2,3, leucodermia puntacta de membros superiores (OR = 2,9 e cutis romboidalis nuchae (OR = 1,8 associaram-se à neoplasia independentemente das demais variáveis, sugerindo um fenótipo de risco. Houve ainda associação com fenótipos claros, genética familiar e exposição solar cumulativa. Queimadura solar, tabagismo e alcoolismo não foram identificados como fatores de risco. O uso de fotoprotetores não evidenciou proteção; porém, o grupo controle era composto por pacientes dermatológicos, aos quais são indicados fotoprotetores regularmente. CONCLUSÃO: Lesões actínicas foram mais prevalentes em portadores de carcinoma basocelular sólido do segmento cefálico que em controles, especialmente mílio, cutis romboidalis nuchae e leucodermia puntacta, independentemente dos demais fatores de risco conhecidos.OBJECTIVE: To evaluate the prevalence of actinic skin lesions in patients with basal cell carcinoma of the head. METHODS: A case-control study was carried out. Cases were patients with primary, solid basal cell carcinoma of the head, less than two centimeters in diameter; and as controls, patients with other dermatoses. Constitutional and behavioral variables were analyzed, as well as actinic lesions. RESULTS: One hundred twenty cases and 360 controls were evaluated. Facial milia (OR = 2.3, leukoderma punctata of the upper limbs (OR = 2.9, and cutis rhomboidalis nuchae (OR = 1.8 were associated with neoplasms regardless

  5. Soil invertebrate community change over fuel-contaminated sites on a subantarctic island: An ecological field-based line of evidence for site risk assessment.

    Science.gov (United States)

    Wasley, Jane; Mooney, Thomas J; King, Catherine K

    2016-04-01

    A number of fuel spills, of both recent and historic origins, have occurred on World Heritage-listed subantarctic Macquarie Island. Sites contaminated by mainly diesel fuels are undergoing remediation by the Australian Antarctic Division. The risks posed by these sites are being managed using a "weight of evidence" approach, for which this study provides a preliminary line of evidence for the ecological assessment component of this site management decision framework. This knowledge is pertinent, given the absence of environmental guidelines for fuel contaminants in subantarctic ecosystems. We provide a field-based, site-specific ecological risk assessment for soil invertebrate communities across the fuel spill sites, before the commencement of in situ remediation activities. Springtails (Collembola) were the most abundant taxa. Springtail community patterns showed only limited correlations with the level of fuel contamination at the soil surface, even when elevated levels occurred in the substratum layers. Of the environmental variables measured, community patterns were most strongly correlated with vegetation cover. We identify a suite of 6 species that contribute most to the community dynamics across these sites. A subset of these we propose as useful candidates for future development of single-species toxicity tests: Folsomotoma punctata, Cryptopygus caecus, Cryptopygus antarcticus and Parisotoma insularis. Findings from this study advance our understanding of soil invertebrate community dynamics within these contaminated sites, directly contributing to the improved management and restoration of the sites. Not only does this study provide an important line of evidence for the island's ecological risk assessment for fuel contaminants, it also enhances our understanding of the potential impact of fuels at other subantarctic islands. Integr Environ Assess Manag 2016;12:306-314. © 2015 SETAC. PMID:26202610

  6. Physiological and molecular characterization of Si uptake in wild rice species.

    Science.gov (United States)

    Mitani-Ueno, Namiki; Ogai, Hisao; Yamaji, Naoki; Ma, Jian Feng

    2014-07-01

    Cultivated rice (Oryza sativa) accumulates high concentration of silicon (Si), which is required for its high and sustainable production. High Si accumulation in cultivated rice is achieved by a high expression of both influx (Lsi1) and efflux (Lsi2) Si transporters in roots. Herein, we physiologically investigated Si uptake, isolated and functionally characterized Si transporters in six wild rice species with different genome types. Si uptake by the roots was lower in Oryza rufipogon, Oryza barthii (AA genome), Oryza australiensis (EE genome) and Oryza punctata (BB genome), but similar in Oryza glumaepatula and Oryza meridionalis (AA genome) compared with the cultivated rice (cv. Nipponbare). However, all wild rice species and the cultivated rice showed similar concentration of Si in the shoots when grown in a field. All species with AA genome showed the same amino acid sequence of both Lsi1 and Lsi2 as O. sativa, whereas species with EE and BB genome showed several nucleotide differences in both Lsi1 and Lsi2. However, proteins encoded by these genes also showed transport activity for Si in Xenopus oocyte. The mRNA expression of Lsi1 in all wild rice species was lower than that in the cultivated rice, whereas the expression of Lsi2 was lower in O. rufipogon and O. barthii but similar in other species. Similar cellular localization of Lsi1 and Lsi2 was observed in all wild rice as the cultivated rice. These results indicate that superior Si uptake, the important trait for rice growth, is basically conserved in wild and cultivated rice species. PMID:24320720

  7. Occurrence of Soft and Hard Ticks on Ruminants in Zagros Mountainous Areas of Iran

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    S Nabian

    2008-08-01

    Full Text Available Background:The distribution and preferences of ticks of animals in central of Iran were studied four times a year. Methods: One thousand seven tick specimens were collected from different localities including Isfahan, Chaharmahalbak­htiary, EastAzarbaijan, West Azerbaijan, Kordestan, Kermanshah, Lorestan and Fars. Results: Most of sampled animals in this area were infested. We also encountered with a wild goat (Capra hircus aegagrus in Kolah Qazi national park in this part that was infested intensively by Boophilus kohlsi. Fifteen ixodid tick species were identified over the study period from cattle, sheep and domestic and wild goats namely B. kohlsi (3.6% Rhipicephalus sanguineus (4.5%, Rh.bursa (21.9%, Rh.turanicus (2.9%, Dermacentor niveus (12.9%, D.raskemensis (4.1%, D.marginatus (7.3%, Haemaphysalis punctata (3.5%, H. Parva (0.6%, H. Choldokovskyi (2%, Hyalomma anatolicum excavatum (4.8%, H.anatolicum anatolicum (5.2%, H. asiaticum asiaticum (7.3%, H. marginatum marginatum (13%, and H. detritum detritum (5.9%. The only soft tick found was Ornithodoros canestrinii which occurred in all localities of Isfahan Province but with significant differences in abundance. Clear pattern of seasonality was evident for this species and it was generally present from November to March, while ixodid ticks were present throughout the year. The largest numbers of adult ixodid ticks were generally present from April to August. Conclusion: The results showed that Rh.bursa, D.niveus and H.marginatum marginatum are dominant tick species

  8. The community structure and seasonal dynamics of plankton in Bange Lake, northern Tibet, China

    Science.gov (United States)

    Zhao, Wen; Zhao, Yuanyi; Wang, Qiaohan; Zheng, Mianping; Wei, Jie; Wang, Shan

    2016-02-01

    The seasonal variations in biomass, abundance, and species composition of plankton in relation to hydrography were studied in the saline Bange Lake, northern Tibet, China. Sampling was carried out between one to three times per month from May 2001 to July 2002. Salinity ranged from 14 to 146. The air and water temperature exhibited a clear seasonal pattern, and mean annual temperatures were approximately 4.8°C and 7.3°C, respectively. The lowest water temperature occurred in winter from December to March at -2°C and the highest in June and July at 17.7°C. Forty-one phytoplankton taxa, 21 zooplankton, and 5 benthic or facultative zooplankton were identified. The predominant phytoplankton species were Gloeothece linearis, Oscillatoria tenuis, Gloeocapsa punctata, Ctenocladus circinnatus, Dunaliella salina, and Spirulina major. The predominant zooplankton species included Holophrya actra, Brachionus plicatilis, Daphniopsis tibetana, Cletocamptus dertersi, and Arctodiaptomus salinus. The mean annual total phytoplankton density and biomass for the entire lake were 4.52×107 cells/L and 1.60 mg/L, respectively. The annual mean zooplankton abundance was 52, 162, 322, and 57, 144 ind./L, in the three sublakes. The annual mean total zooplankton biomass in Lakes 1-3 was 1.23, 9.98, and 2.13 mg/L, respectively. The annual mean tychoplankton abundances in Bg1, 2, and 3 were 47, 67, and 654 ind./L. The annual mean tychoplankton biomass was 2.36, 0.16, and 2.03 mg/L, respectively. The zooplankton biomass (including tychoplankton) in the lake was 9.11 mg/L. The total number of plankton species in the salt lake was significantly negatively correlated with salinity.

  9. The Ostracoda assemblage of the Eocene-Oligocene transition in northwestern Thrace: Kırklareli-Edirne area (northwestern Turkey)

    Science.gov (United States)

    Şafak, Ümit; Güldürek, Manolya

    2016-05-01

    The aim of the study is to investigate the Eocene-Oligocene transition in detail in northwest Thrace (NW Turkey) with heavy reliance on ostracod fauna. The lithologies formed and the environmental changes during this time period were also studied. The study was carried out in northwest Thrace within the outcropping Koyunbaba, Soğucak, and Ceylan Formations; Mezardere, Osmancık, and Danişmen Formations of the Yenimuhacir Group; and the Taşlısekban and Pınarhisar members of the Danişmen Formation. Rich ostracod fauna indicating an Eocene and Oligocene age and environment are found within these units. The Ostracoda fauna identified were ostracods Triebelina punctata, Bairdia cymbula, Bairdia tenuis, Cyamocytheridea nova, Krithe bartonensis, Krithe angusta, Krithe rutoti, Krithe parvula, Echinocythereis isabenana, Leguminocythereis genappensis, Grinioneis triebeli, Xestoleberis subglobosa and Xestoleberis muelleriana from the Mid-Late Eocene epoch; Cytheromorpha zinndorfi, Hemicyprideis montosa, Neocyprideis williamsoniana, Cladarocythere apostolescui, Hammatocythere hebertiana, Haplocytheridea helvetica, Cytheridea pernota, Callistocythereis vitilis, Cushmanidea cf. scrobiculata, Pterygocythereis fimbriata, Pokornyella limbata, Grinioneis paijenborchiana, Cytheretta tracensis, Macrocypris wrightii and Paracypris bouldnorensis from the Late Eocene-Early Oligocene epoch; and Novocypris eocenana, Novocypris striata, Moenocypris forbesi, Candona (Pseudocandona) fertilis, Candona (Lineocypris) sp. and Cypridopsis soyeri from the Early-Late Oligocene epoch. The study was also correlated to previous research conducted on Eocene-Oligocene age ostracods around the area, in northwestern Europe, and in the Paris-Akiten Basin, in view of similar age-environment relationships determined by said studies. On the basis of evidence from the lithologic content of the beds and the micropaleontological investigation, the fossil community identified in this study indicates that the

  10. The Subtelomere of Oryza sativa Chromosome 3 Short Arm as a Hot Bed of New Gene Origination in Rice

    Institute of Scientific and Technical Information of China (English)

    Chuanzhu Fan; Yong Zhang; Yeisoo Yu; Steve Rounsley; Manyuan Long; Rod A.Wing

    2008-01-01

    Despite general observations of non-random genomic distribution of new genes,it is unclear whether or not new genes preferentially occur in certain genomic regions driven by related molecular mechanisms.Using 1.5 Mb of genomic sequences from short arms of chromosome 3 of Oryza glaberrima and O.punctata,we conducted a comparative genomic analysis with the reference O.sativa ssp-japonica genome.We identified a 60-kb segment Iocated in the middle of the subtelomeric region of chromosome 3,which is unique to the species O.sativa.The region contained gene duplicares that occurred in Asian cultivated rice species that diverged from the ancestor of Asian and African cultivated rice one million years ago(MYA).For the 12 genes and one complete retrotransposon identified in this segment in O.sativa ssp.japonica,we searched for their parental genes.The high similarity between duplicated paralogs further supports the recent origination of these genes.We found that this segment was recently generated through multiple independent gene recombination and transposon insertion events.Among the 12 genes,we found that five had chimeric gene structures derived from multiple parental genes.Nine out of the 12 new genes seem to be functional,as suggested by Ka/Ks analysis and the presence of cDNA and/or MPSS data.Furthermore,for the eight transcribed genes,at least two genes could be classified as defense or stress response-related genes.Given these findings,and the fact that subtelomeres are associated with high rates of recombination and transcription,it is likely that subtelomeres may facilitate gene recombination and transposon insertions and serve as hot spots for new gene origination in rice genomes.

  11. The Upper Devonian orbital cyclostratigraphy and numerical dating conodont zones from Guangxi,South China

    Institute of Scientific and Technical Information of China (English)

    GONG Yiming; XU Ran; TANG Zhongdao; LI Baohua

    2005-01-01

    The hierarchically organized laminae, bundles, bundlesets and superbundlesets which correspond to a sub-Milankovitch, obliquity or precession, eccentricity and long eccentricity cyclothems, respectively, have been distinguished from the Upper Devonian Frasnian-Famennian (F-F) transitional carbonate successions deposited in the carbonate-basin and slope facies of Guangxi, South China. The durations of cyclothems are 8000-10000a, 16667a or 33333a, 100000a and 400000a, respectively. The ratio of eccentricity to precession, eccentricity to obliquity, and long eccentricity to eccentricity is 1:6,1:3 and 1 : 4 in the Devonian,respectively. Orbital cyclostratigraphical studies show that the durations of the conodont falsiovalis Zone, transitans Zone, punctata Zone, Lower hassi Zone, Upper hassi Zone, jamieae Zone,Lower rhenana Zone, Upper rhenana Zone,linguiformis Zone, Lower triangularis Zone, Middle triangularis Zone and Upper triangularis Zone are 0.4, 0.4, 0.4, 0.3, 0.4, 0.2, 0.8, 0.6, 0.8, 0.3, 0.3and 0.3 Ma from bottom to top, respectively, and the duration of the Frasnian is 4.3 Ma. The conodont is the normal marine organism of the latest mass extinction (the latest linguiformis Zone)and the first recovery (including the whole Lower triangularis and Middle triangularis Zone) in the F-F transition. The conodont mass extinction and recovery lasted 200000-100000a and 0.6 Ma,respectively. We consider that average durations of the fossil zones calculated by reported numerical ages divided by fossil zone numbers within a stage or series or system cannot discovery complications and non-uniformity of evolutionary organisms and environments.

  12. Palaeolimnological assessment of lake acidification and environmental change in the Athabasca Oil Sands Region, Alberta

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    Sergi PLA

    2010-08-01

    Full Text Available Exploitation of the Athabasca Oil Sands has expanded hugely over the last 40 years. Regional emissions of oxidised sulphur and nitrogen compounds increased rapidly over this period and similar emissions have been linked to lake acidification in other parts of North America and Europe. To determine whether lakes in the region have undergone acidification, 12 lakes within the Regional Municipality of Wood Buffalo and the Caribou Mountains were selected to cover chemical and spatial gradients and sediment cores were obtained for palaeolimnological analyses including radiometric dating, diatom analysis, isotopic analysis of bulk sediment 13C and 15N, and spheroidal carbonaceous particles (SCPs. All lake sediment cores show evidence of industrial contamination based on SCPs, but there is no clear industrial signal in stable isotopes. Most lakes showed changes in diatom assemblages and sediment C:N ratios consistent with nutrient enrichment over various timescales, with potential drivers including climatic change, forest fires and anthropogenic nitrogen deposition. Only one of the 12 lakes investigated showed strong evidence of acidification with a decline in diatom-inferred pH from 6.3 to 5.6 since 1970 linked to increasing relative abundances of the acidophilous diatom species Actinella punctata, Asterionella ralfsii and Fragilariforma polygonata. Analysis of mercury (Hg in the acidified lake showed increasing sediment fluxes over the last 20 years, a possible indication of industrial contamination. The acidified lake is the smallest of those studied with the shortest residence time, suggesting a limited capacity for neutralisation of acid inputs in catchment soils or by inlake processes.

  13. Calibrating snakehead diversity with DNA barcodes: expanding taxonomic coverage to enable identification of potential and established invasive species.

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    Natasha R Serrao

    Full Text Available Detecting and documenting the occurrence of invasive species outside their native range requires tools to support their identification. This can be challenging for taxa with diverse life stages and/or problematic or unresolved morphological taxonomies. DNA barcoding provides a potent method for identifying invasive species, as it allows for species identification at all life stages, including fragmentary remains. It also provides an efficient interim taxonomic framework for quantifying cryptic genetic diversity by parsing barcode sequences into discontinuous haplogroup clusters (typical of reproductively isolated species and labelling them with unique alphanumeric identifiers. Snakehead fishes are a diverse group of opportunistic predators endemic to Asia and Africa that may potentially pose significant threats as aquatic invasive species. At least three snakehead species (Channa argus, C. maculata, and C. marulius are thought to have entered North America through the aquarium and live-food fish markets, and have established populations, yet their origins remain unclear. The objectives of this study were to assemble a library of DNA barcode sequences derived from expert identified reference specimens in order to determine the identity and aid invasion pathway analysis of the non-indigenous species found in North America using DNA barcodes. Sequences were obtained from 121 tissue samples representing 25 species and combined with public records from GenBank for a total of 36 putative species, which then partitioned into 49 discrete haplogroups. Multiple divergent clusters were observed within C. gachua, C. marulius, C. punctata and C. striata suggesting the potential presence of cryptic species diversity within these lineages. Our findings demonstrate that DNA barcoding is a valuable tool for species identification in challenging and under-studied taxonomic groups such as snakeheads, and provides a useful framework for inferring invasion pathway

  14. Calibrating snakehead diversity with DNA barcodes: expanding taxonomic coverage to enable identification of potential and established invasive species.

    Science.gov (United States)

    Serrao, Natasha R; Steinke, Dirk; Hanner, Robert H

    2014-01-01

    Detecting and documenting the occurrence of invasive species outside their native range requires tools to support their identification. This can be challenging for taxa with diverse life stages and/or problematic or unresolved morphological taxonomies. DNA barcoding provides a potent method for identifying invasive species, as it allows for species identification at all life stages, including fragmentary remains. It also provides an efficient interim taxonomic framework for quantifying cryptic genetic diversity by parsing barcode sequences into discontinuous haplogroup clusters (typical of reproductively isolated species) and labelling them with unique alphanumeric identifiers. Snakehead fishes are a diverse group of opportunistic predators endemic to Asia and Africa that may potentially pose significant threats as aquatic invasive species. At least three snakehead species (Channa argus, C. maculata, and C. marulius) are thought to have entered North America through the aquarium and live-food fish markets, and have established populations, yet their origins remain unclear. The objectives of this study were to assemble a library of DNA barcode sequences derived from expert identified reference specimens in order to determine the identity and aid invasion pathway analysis of the non-indigenous species found in North America using DNA barcodes. Sequences were obtained from 121 tissue samples representing 25 species and combined with public records from GenBank for a total of 36 putative species, which then partitioned into 49 discrete haplogroups. Multiple divergent clusters were observed within C. gachua, C. marulius, C. punctata and C. striata suggesting the potential presence of cryptic species diversity within these lineages. Our findings demonstrate that DNA barcoding is a valuable tool for species identification in challenging and under-studied taxonomic groups such as snakeheads, and provides a useful framework for inferring invasion pathway analysis. PMID

  15. The nematode stoma: Homology of cell architecture with improved understanding by confocal microscopy of labeled cell boundaries.

    Science.gov (United States)

    Jay Burr, A H; Baldwin, James G

    2016-09-01

    Nematode stomas vary widely in the cuticular structures evolved for different feeding strategies, yet the arrangement of the epithelial cell classes that form these structures may be conserved. This article addresses several issues that have impeded the full acceptance of this hypothesis including controversies arising from the structure of the Caenorhabditis elegans stoma. We investigated fluorescent antibody labeling of cell boundaries in conjunction with confocal microscopy as an alternative to transmission electron microscopy (TEM), using MH27 to label apical junctions in C. elegans and two other species. Accurately spaced optical sections collected by the confocal microscope provide a three-dimensional array of pixels (voxels) that, using image-processing software, can be rotated and sectioned at accurately chosen thicknesses and locations. Ribbons of fluorescence clearly identify cell boundaries along the luminal cuticle in C. elegans and Zeldia punctata and less clearly in Bunonema sp. The patterns render cell classes and their relationships readily identifiable. In the C. elegans stoma they correct a misreading of serial TEMs that was not congruent with architecture in other nematodes-the row of marginal cells is now seen to be continuous as in other nematodes, rather than being interrupted by encircling pm1 cells. Also impeding understanding, the reference to certain cell classes as 'epithelial' and others as "muscle" in the C. elegans literature is at variance with muscle expression in most other taxa. For consistent comparison among species, we propose that these cell class descriptors based on function be replaced by topological terms. With these and other confusing concepts and terminology removed, the homology of the cellular architecture among taxa becomes obvious. We provide a corrected description of the cell architecture of the C. elegans stoma and examples of how it is modified in other taxa with different feeding strategies. J. Morphol. 277

  16. Mechanical transmission of pathogenic organisms: the role of cockroaches

    Directory of Open Access Journals (Sweden)

    Y.M. Tatfeng, M.U. Usuanlele, A. Orukpe, A.K. Digban, M. Okodua,F. Oviasogie & A.A. Turay

    2005-12-01

    Full Text Available Background & objectives: Cockroaches (Diploptera punctata are basically tropical insects and willdo their best to find a home that is both warm and moist. Their involvement in the transmission oftropical diseases is poorly investigated in Africa.Methods: A study on the bacterial, fungal and parasitic profile of cockroaches trapped in and aroundhouses in Ekpoma was carried out using standard microbiological techniques.Results: Of a total of 234 cockroaches trapped from different sites (toilets, parlours, kitchens andbedrooms in houses with pit latrines and water system, the bacterial, fungal and parasitic isolateswere identical irrespective of the site, these included: E. coli, Klebsiella pneumoniae, Proteus vulgaris,Proteus mirabilis, Citrobacter freundii, Enterobacter cloacae, Salmonella sp, Pseudomonasaeruginosa, Serretia marcescens, S. aureus, S. feacalis, S. epidermidis, Aeromonas sp, Candida sp,Rhizopus sp, Aspergillus sp, Mucor sp, cysts of E. hystolitica, oocysts of C. parvum, C. cayetenensisand Isospora belli, cysts of Balantidium coli, ova of Ascaris lumbricoides, Anchylostoma deodunalae,Enterobius vermicularis, ova Trichuris trichura, larva of Strongyloides stercoralis. Cockroachestrapped in the toilets of houses with pit latrines had a mean bacterial and parasites count of12.3 × 1010 org/ml and 98 parasites/ml respectively, while those trapped in the houses with watersystem had a mean bacterial and parasitic count of 89.5 × 107 org/ml and 31 parasites/ml respectively.A bacterial count of 78.9 × 107 org/ml was recorded from cockroaches trapped from the kitchens ofhouses with pit latrines. On the other hand a mean bacterial and parasitic count of 23.7 × 106 org/mland 19 parasites/ml were recorded from kitchens of houses with water system.Interpretation & conclusion: Cockroaches represent an important reservoir for infectious pathogens,therefore, control of cockroaches will substantially minimise the spread of infectious diseases

  17. Evaluation of clinical safety and anthelmintic efficacy of aurixazole administed orally at 24 mg/kg in cattle.

    Science.gov (United States)

    Sakamoto, Claudio Alessandro M; Lopes, Welber Daniel Zanetti; Buzzulini, Carolina; Cruz, Breno Cayeiro; Felippelli, Gustavo; Teixeira, Weslen F; Silva, Helenara Machado; Santana, Luis Fernando; Soares, Vando Edésio; Henrique, Carlos Henrique; de Oliveira, Gilson Pereira; da Costa, Alvimar José

    2014-06-01

    The current study evaluated, in vivo, the clinical safety and the anthelmintic efficacy of 24% aurixazole (24 mg/kg), administered orally, in bovines. Two experiments were conducted: the first one evaluating the clinical safety of 24% aurixazole (24 mg/kg) in cattle, and a second one evaluating the anthelmintic efficacy of aurixazole (24 mg/kg) against gastrointestinal nematodes on naturally infected cattle. Based on the results of clinical safety, no alterations on clinical and haematological signs and on the biochemical values obtained in animals treated orally with aurixazole 24 mg/kg were observed. Regarding the results of reduction or efficacy, obtained by eggs per gram of faeces (EPG) counts, the formulation of aurixazole reached values superior to 99% (arithmetic means) in all post-treatment dates. In two occasions, this formulation reached maximum efficacy (100%). Comparing these results with the reduction percentages obtained by EPG counts, it is possible to verify that the values obtained by all three formulations were compatible with the efficacy results. Aurixazole reached maximum efficacy (100%) against Haemonchus placei, Cooperia spatulata and Oesophagostomum radiatum. Against Cooperia punctata, this formulation reached an efficacy index of 99.99%. Regarding aurixazole, no specific trials were conducted on the field in order to evaluate the behaviour of this molecule against helminths that are resistant to other molecules, specially isolated levamisole and disophenolat. Due to this fact, future studies will be necessary to assess the effectiveness of aurixazole against strains of nematodes that are resistant to levamisole and disophenolat, but the results of clinical safety and efficacy described in this study allow us to conclude that the aurixazole molecule, concomitantly with other measures and orally administered formulations, can be another important tool in the control of nematodes parasitizing bovines. PMID:24656344

  18. Integrative Taxonomic Study of the Purse Crab Genus Persephona Leach, 1817 (Brachyura: Leucosiidae): Combining Morphology and Molecular Data

    Science.gov (United States)

    Magalhães, Tatiana; Robles, Rafael; Felder, Darryl L.

    2016-01-01

    Marine crabs of the genus Persephona Leach, 1817 are restricted to American waters of the western Atlantic and eastern Pacific Oceans. Subfamilial assignment of this taxon has varied between authors and its species composition remain in question. We conducted a comparative study based on morphology and molecular phylogenetics for all ten recognized species of Persephona, along with Iliacantha hancocki. We tested whether Persephona finneganae, P. lichtensteinii, and P. crinita represent a single species as suggested by some authors; whether specimens identified as P. punctata, P. mediterranea, and P. aquilonaris warrant treatment as separate species; and whether I. hancocki should be regarded as a junior synonym of P. subovata. Diagnostic morphological characters (of the carapace, chelipeds, and third maxillipeds) were used along with gonopod (male first pleopod 1) features and live coloration. The 16S rRNA and the Cytochrome Oxidase I (COI) (DNA barcoding) mitochondrial genes were used as molecular markers. Both morphological and molecular analyses revealed that putative specimens of P. crinita from Brazil and those assigned to P. finneganae were no different from specimens presently assignable to P. lichtensteinii. P. finneganae is regarded as a junior synonym of P. lichtensteinii, and we apply P. crinita only to specimens we examined from the Gulf of Mexico. Specimens from Brazil previously reported as P. crinita are herewith concluded to represent P. lichtensteinii. Additionally, P. townsendi is a junior synonym of P. orbicularis, Iliacantha hancocki is concluded to be a junior synonym of P. subovata, while P. aquilonaris and P. mediterranea are found to represent separate species. On the basis of our revisions, eight species of Persephona are considered valid, and the reported distribution for P. crinita is restricted. PMID:27099956

  19. Moraea intermedia and M. vuvuzela (Iridaceae-Iridoideae, two new species from western South Africa, and some nomenclatural changes and range extensions in the genus

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    P. Goldblatt

    2010-12-01

    Full Text Available We describe two new species in the largely sub-Saharan genus Moraea Mill. (± 205 spp. from its centre of diversity in the winter rainfall region of southern Africa. Moraea intermedia, from north-central Namaqualand near Springbok, is a member of the small section Tubiflorae (now eight species, remarkable in its growth habit with a long basal intemode. leaves clustered at the first aerial node, and Moraea-type stamens and style branches but subequal tepals with very short claws that clasp only the base of the filament column. Moraea vuvuzela. a member of series Galaxia of the Galaxia group of the genus (now 17 species, has deeply fringed stigma lobes, filaments free in the upper 1 mm, ± prostrate, lanceolate leaves and. remarkable for the series, dark brown to purple markings near the base of the tepal limbs. In the unusually variable M.fugax, currently with two subspecies, new collections of subsp. fugax co-occurring but on different soils with subsp.  filicaulis, cast doubt on their current treatment as members of the same species. We now favour recognition of the diminutive subsp.filicaulis as a separate species, M. filicaulis. In the M iripetala group we recommend recognition of the early blooming M. punctata, described in 1892 and later subsumed in M. iripetala but readily distinguished by the long inner  tepals broader in the midline and short, relatively broad, plane rather than channelled leaves. We also report small but significant range extensions for M. barkerae, M. macrocarpa and M. tricolor.

  20. XBP1-Independent UPR Pathways Suppress C/EBP-β Mediated Chondrocyte Differentiation in ER-Stress Related Skeletal Disease.

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    Trevor L Cameron

    2015-09-01

    Full Text Available Schmid metaphyseal chondrodysplasia (MCDS involves dwarfism and growth plate cartilage hypertrophic zone expansion resulting from dominant mutations in the hypertrophic zone collagen, Col10a1. Mouse models phenocopying MCDS through the expression of an exogenous misfolding protein in the endoplasmic reticulum (ER in hypertrophic chondrocytes have demonstrated the central importance of ER stress in the pathology of MCDS. The resultant unfolded protein response (UPR in affected chondrocytes involved activation of canonical ER stress sensors, IRE1, ATF6, and PERK with the downstream effect of disrupted chondrocyte differentiation. Here, we investigated the role of the highly conserved IRE1/XBP1 pathway in the pathology of MCDS. Mice with a MCDS collagen X p.N617K knock-in mutation (ColXN617K were crossed with mice in which Xbp1 was inactivated specifically in cartilage (Xbp1CartΔEx2, generating the compound mutant, C/X. The severity of dwarfism and hypertrophic zone expansion in C/X did not differ significantly from ColXN617K, revealing surprising redundancy for the IRE1/XBP1 UPR pathway in the pathology of MCDS. Transcriptomic analyses of hypertrophic zone cartilage identified differentially expressed gene cohorts in MCDS that are pathologically relevant (XBP1-independent or pathologically redundant (XBP1-dependent. XBP1-independent gene expression changes included large-scale transcriptional attenuation of genes encoding secreted proteins and disrupted differentiation from proliferative to hypertrophic chondrocytes. Moreover, these changes were consistent with disruption of C/EBP-β, a master regulator of chondrocyte differentiation, by CHOP, a transcription factor downstream of PERK that inhibits C/EBP proteins, and down-regulation of C/EBP-β transcriptional co-factors, GADD45-β and RUNX2. Thus we propose that the pathology of MCDS is underpinned by XBP1 independent UPR-induced dysregulation of C/EBP-β-mediated chondrocyte differentiation

  1. Sustainable control of onchocerciasis: ocular pathology in onchocerciasis patients treated annually with ivermectin for 23 years: a cohort study.

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    Méba Banla

    Full Text Available The evolution and persistence of ocular pathology was assessed in a cohort of Onchocerca volvulus infected patients treated annually with ivermectin for 23 years. Patients were resident in rural Central and Kara Region of Togo and ocular examinations included testing of visual acuity, slit lamp examination of the anterior eye segment and the eye fundus by ophthalmoscopy. Before ivermectin treatment, vivid O.volvulus microfilariae (MF were observed in the right and left anterior eye chamber in 52% and 42% of patients (n = 82, and dead MF were seen in the right and left cornea in 24% and 15% of cases, respectively. At 23 years post initial treatment (PIT, none of the patients (n = 82 presented with MF in the anterior chamber and cornea. A complete resolution of punctate keratitis (PK lesions without observable corneal scars was present at 23 years PIT (p<0.0001, and sclerosing keratitits (SK lessened by half, but mainly in patients with lesions at early stage of evolution. Early-stage iridocyclitis diminished from 42%(rE and 40%(lE to 13% (rE+lE(p<0.0001, but advanced iridocyclitis augmented (p<0.001 at 23 years PIT compared to before ivermectin. Advanced-stage papillitis and chorioretinitis did not regress, while early-stage papillitis present in 28%(rE and 27%(lE of patients at before ivermectin regressed to 17%(rE and 18%(lE, and early-stage chorioretinitis present in 51%(rE+lE of cases at before ivermectin was observed in 12%(rE and 13%(lE at 23 years PIT (p<0.0001. Thus, regular annual ivermectin treatment eliminated and prevented the migration of O. volvulus microfilariae into the anterior eye chamber and cornea; keratitis punctata lesions resolved completely and early-stage sclerosing keratitits and iridocyclitis regressed, whilst advanced lesions of the anterior and posterior eye segment remained progressive. In conclusion, annual ivermectin treatments may prevent the emergence of ocular pathology in those populations still exposed

  2. Molecular identification of Borrelia genus in questing hard ticks from Portugal: Phylogenetic characterization of two novel Relapsing Fever-like Borrelia sp.

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    Nunes, Mónica; Parreira, Ricardo; Maia, Carla; Lopes, Nádia; Fingerle, Volker; Vieira, M Luísa

    2016-06-01

    In the last decades, several studies have reported pathogenic species of Borrelia related to those that cause Tick-borne Relapsing Fever (RF), but unexpectedly suggesting their transmission by hard ticks, known vectors of Borrelia burgdorferi sensu lato (B. burgdorferi s.l.) species, rather than by soft ticks. This study was designed to update the presence of B. burgdorferi s.l. species in ticks from several districts of mainland Portugal, where Ixodes ricinus had been previously described. Ticks (a total of 2915 specimens) were collected in seven districts throughout the country, and analyzed using molecular methods. Three nested-PCR protocols, targeting the flagellin gene (flaB), the intergenic spacer region (IGS) located between 5S and 23S rRNA, and the glpQ gene, and a conventional PCR targeting the 16S rRNA, were used for Borrelia DNA detection. Borrelia DNA was detected in 3% of the ticks from Braga, Vila Real, Lisboa, Setúbal, Évora and Faro districts. The obtained amplicons were sequenced and analyzed by BLASTn, and 15/63 (24%) matched with homologous sequences from Borrelia lusitaniae and 15/63 (24%) with B. garinii, being these the most prevalent species. DNA from B. burgdorferi sensu stricto (s.s.), B. valaisiana and B. afzelii were detected in 7/63 (11%), 6/63 (10%), and 2/63 (3%) of the specimens, respectively. Unexpectedly, DNA sequence (flaB) analysis from eight (13%) samples, two from Rhipicephalus sanguineus and six from Haemaphysalis punctata tick species, revealed high homology with RF-like Borrelia. Phylogenetic analyses obtained from three genetic markers (16S rRNA, flaB, and glpQ) confirmed their congruent inclusion in a strongly supported RF cluster, where they segregated in two subgroups which differ from the other Relapsing Fever species. Therefore, the results confirm the circulation of multiple species of B. burgdorferi s.l. over a wide geographic range, covering most of the Portuguese mainland territory. Surprisingly, the obtained data

  3. 太湖流域浮萍种质资源及其生长水环境调查

    Institute of Scientific and Technical Information of China (English)

    张飞; 唐杰; 马炯; 成家杨

    2016-01-01

    对太湖流域多个县(市)浮萍分布情况进行调查,观测各地浮萍种类及其生长水体的水质状况,探讨水体pH值,铵态氮、硝态氮、总氮、总磷含量及其与浮萍分布的关系.调查结果表明,浮萍适宜生长在静止的水体中,流动的河流中较为少见.太湖流域采集到3属5种浮萍,分别为Spirodela polyrrhiza、Lemna minor、Lemna turionifera、Lemna aequinoctialis、Landoltia punctata.其中,紫萍属(Spirodela genera)和青萍属(Lemna genera)分别占到49%和37%,为该地区主要的浮萍种类.少根紫萍适宜在中性的环境中生长,而紫萍适宜生长在偏碱性的水体中;相对而言,青萍生长的pH范围更广,在pH值6.34 ~ 8.78均有分布.太湖流域浮萍生长水环境中氮、磷含量范围较广,铵态氮、硝态氮、总氮和总磷质量浓度范围分别为0.28~19.67、0.01 ~5.18、0.10 ~24.62、0.03 ~ 2.45 mg/L.尚未发现自然环境中浮萍的分布与水体氮、磷含量间的必然联系.调查还发现,太湖流域浮萍淀粉含量在10.95%~23.27%之间,均值为15.95%.在太湖流域水生植物修复过程中,可优先考虑利用当地优势浮萍品种及不同品种浮萍的共生复合系统,构建浮萍塘生态系统,来去除水体中的氮、磷等营养物质.

  4. Polyploid evolution in Oryza officinalis complex of the genus Oryza

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    Li Changbao

    2009-10-01

    Full Text Available Abstract Background Polyploidization is a prominent process in plant evolution, whereas the mechanism and tempo-spatial process remained poorly understood. Oryza officinalis complex, a polyploid complex in the genus Oryza, could exemplify the issues not only for it covering a variety of ploidy levels, but also for the pantropical geographic pattern of its polyploids in Asia, Africa, Australia and Americas, in which a pivotal genome, the C-genome, witnessed all the polyploidization process. Results Tracing the C-genome evolutionary history in Oryza officinalis complex, this study revealed the genomic relationships, polyploid forming and diverging times, and diploidization process, based on phylogeny, molecular-clock analyses and fluorescent in situ hybridization using genome-specific probes. Results showed that C-genome split with B-genome at ca. 4.8 Mya, followed by a series of speciation of C-genome diploids (ca. 1.8-0.9 Mya, which then partook in successive polyploidization events, forming CCDD tetraploids in ca. 0.9 Mya, and stepwise forming BBCC tetraploids between ca. 0.3-0.6 Mya. Inter-genomic translocations between B- and C-genomes were identified in BBCC tetraploid, O. punctata. Distinct FISH (fluorescent in situ hybridization patterns among three CCDD species were visualized by C-genome-specific probes. B-genome was modified before forming the BBCC tetraploid, O. malampuzhaensis. Conclusion C-genome, shared by all polyploid species in the complex, had experienced different evolutionary history particularly after polyploidization, e.g., inter-genomic exchange in BBCC and genomic invasion in CCDD tetraploids. It diverged from B-genome at 4.8 Mya, then participated in the tetraploid formation spanning from 0.9 to 0.3 Mya, and spread into tropics of the disjunct continents by transcontinentally long-distance dispersal, instead of vicariance, as proposed by this study, given that the continental splitting was much earlier than the C

  5. Brachiopods and stratigraphy of the Upper Devonian (Frasnian) succession of the Radlin Syncline (Holy Cross Mountains, Poland)

    Science.gov (United States)

    Baliński, Andrzej; Racki, Grzegorz; Halamski, Adam T.

    2016-06-01

    The lower part of the Frasnian succession in the Radlin Syncline (Kielce-Łagów Synclinorium, southern region of the Holy Cross Mountains), in the two studied successions: Józefka at Górno and (for the first time) Radlin, consists of the rhythmic marly Szydłówek Beds, the fossil-rich limestones of the Wietrznia Beds (locally) and the atypically developed, calcareous Kostomłoty Beds. The carbon isotope chemostratigraphic pattern overall corresponds well to the global Early-Middle Frasnian biogeochemical perturbation, even if the major punctata positive excursion is only fragmentarily recorded in the Kostomłoty intrashelf basin. Two brachiopod assemblages are abundantly represented in both sections: the Phlogoiderhynchus polonicus Assemblage, typical of the Szydłówek Beds, and the Biernatella lentiformis Assemblage, limited to the middle part of the Wietrznia Beds. Both are highly dominated by the index species. Twenty nine lower Frasnian brachiopod species (Craniida - 1 species, Strophomenida - 1, Productida - 2, Protorthida - 1, Orthida - 5, Pentamerida - 1, Rhynchonellida - 4, Atrypida - 4, Athyridida - 3, Spiriferida - 4, Spiriferinida - 3) are described from the Szydłówek and Wietrznia Beds. Seven new species are introduced: Skenidioides cretus Halamski sp. nov., Biernatium minus Baliński sp. nov., Monelasmina montisjosephi Baliński sp. nov., Atryparia (Costatrypa) agricolae Halamski and Baliński sp. nov., Davidsonia enmerkaris Halamski sp. nov., Leptathyris gornensis Baliński sp. nov., and Echinocoelia parva Baliński sp. nov. Davidsonia enmerkaris Halamski sp. nov. is intermediate between Davidsonia Bouchard-Chantereaux, 1849 and Rugodavidsonia Copper, 1996 and is the youngest known representative of the suborder Davidsonioidea Copper, 1996. Skenidioides cretus Halamski sp. nov. is the last representative of the genus. Statistical investigation of a large sample of Spinatrypina (Exatrypa) explanata did not confirm the existence of two dimorphic

  6. Relação entre desempenho e infecções por nematódeos gastrintestinais em bovinos Nelore em crescimento Relationship between performance and infections by gastrointestinal nematodes in growing Nelore cattle

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    C.V.J. Nicolau

    2002-08-01

    Full Text Available Este estudo teve por objetivo verificar a associação entre o desempenho e a carga parasitária de 28 machos inteiros da raça Nelore (a partir de um ano de idade, naturalmente infectados por nematódeos gastrintestinais. De novembro de 1999 a junho de 2000, os animais foram pesados quinzenalmente, período no qual foram colhidas amostras de fezes e de sangue para a realização, respectivamente, de exames coprológicos e sangüíneos. Os dados da contagem de ovos por grama de fezes (OPG ajustaram-se ao modelo de distribuição binomial negativa, indicando que a distribuição de nematódeos no rebanho é agregada. Não ficou evidenciada associação entre ganho de peso e contagem de OPG, e entre contagem de OPG e volume globular (VG. De 10 coeficientes de correlação entre ganho de peso e VG, oito foram negativos, mas apenas dois significativos (PThis study was carried out to verify the association between the performance and the parasitic load of 28 growing Nelore bulls (one year old naturally infected with gastrointestinal nematodes. From November/1999 to June/2000, the animals were weighed fortnightly and in this period fecal and blood samples were collected for laboratory analyses. The data of the egg counts per gram of feces (EPG fitted into a negative binomial distribution model, indicating that the nematodes exhibit aggregated distribution. There was no clear evidence of association between the weight gain and the EPG or between the EPG counts and the packed cell volume (PCV. Eight of the 10 correlation coefficients between weight gain and PCV were negative, but only two were significant (P<0.05. The estimates of repeatability of the EPG, LOG (EPG + 1 and PCV were of 0.26, 0.25 and 0.33, respectively. Cooperia punctata was the species more frequently found and in heavier load parasitizing the animals. Besides this species, the following nematodes were observed: Haemonchus placei, Haemonchus similis, Trichostrongylus axei, Bunostomum

  7. Endectocide activity of a pour-on formulation containing 1.5 per cent ivermectin +0.5 per cent abamectin in cattle.

    Science.gov (United States)

    Silva, Heloisa Cristina; Prette, Nancy; Lopes, Welber Daniel Zanetti; Sakamoto, Cláudio Alessandro M; Buzzulini, Carolina; Dos Santos, Thais Rabelo; Cruz, Breno Cayeiro; Teixeira, Weslen F Pires; Felippelli, Gustavo; Carvalho, Rafael Silveira; Maciel, Willian Giquelin; Soares, Vando Edésio; da Costa, Alvimar José

    2015-01-01

    The present work aimed to evaluate, through ten different studies, the therapeutic efficacy of a new pour-on formulation, containing 1.5 per cent ivermectin +0.5 per cent abamectin, against parasites of cattle. Results obtained on trials against Rhipicephalus (Boophilus) microplus showed that the pour-on combination of 1.5 per cent ivermectin +0.5 per cent abamectin obtained superior efficacy indexes against this ectoparasite, when compared with formulations containing 0.5 per cent ivermectin, 1 per cent ivermectin and the combination of 1 per cent abamectin +20 per cent levamisole. The results of efficacy of the ivermectin+abamectin and the 0.5 per cent ivermectin against Haematobia irritans were similar. Against Cochliomyia hominivorax larvae, all pour-on formulations tested (1.5 per cent ivermectin +0.5 per cent abamectin, 0.5 per cent ivermectin and 0.5 per cent abamectin), as well as 1 per cent doramectin administered subcutaneously, were considered ineffective. Cattle medicated with 1.5 per cent ivermectin +0.5 per cent abamectin, pour-on, remained free from parasitism by Dermatobia hominis larvae during 42 days (96 per cent efficacy), while values superior to 90 per cent were obtained by 0.5 per cent ivermectin (92 per cent) and 0.5 per cent abamectin (93 per cent) until the 42nd and 35th days post treatment, respectively. Against Haemonchus placei and Oesophagostomum radiatum, the pour-on of ivermectin+abamectin showed better efficacy than the 0.5 per cent ivermectin and 0.5 per cent abamectin. As to Cooperia punctata, there was no difference regarding efficacy results obtained by the avermectins combination and abamectin. The pour-on combination of 1.5 per cent ivermectin +0.5 per cent abamectin obtained high efficacy against R. (B.) microplus, D. hominis and some species of cattle gastrointestinal helminths when compared with formulations of 0.5 per cent ivermectin and 0.5 per cent abamectin administered through the same route. PMID:26392893

  8. Hallazgo de depósitos cretácicos en la Precordillera de La Rioja (Formación Ciénaga del Río Huaco

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    P.L. Ciccioli

    2005-03-01

    Full Text Available En este trabajo se da a conocer el hallazgo de sedimentitas maastrichtianas en la Precordillera septentrional (provincia de La Rioja. Se trata de una secuencia de bancos rojos de unos 122 m de espesor compuesta por areniscas, pelitas, margas y delgados niveles de evaporitas. Estas rocas fueron divididas en seis asociaciones de facies, las que caracterizan en su mayor parte a un sistema lacustre desarrollado bajo condiciones áridas o semiáridas. Probablemente como resultado de un incremento en la aridez, los sedimentos lacustres fueron reemplazados por condiciones de sedimentación eólica bien representadas en el techo de la unidad. La secuencia lacustre muestra al menos dos ciclos de expansión-contracción, el primero es el de mayor desarrollo, dominado durante el estado de máxima inundación por niveles de pelitas negras fosilíferas. Estos últimos han provisto microfósiles calcáreos, palinomorfos y restos de gastrópodos en regular estado de preservación. Los microfósiles calcáreos incluyen charophytas y ostrácodos. Entre las algas (girogonites se han identificado Platychara compressa y Tectochara sp. A. mientras que los ostrácodos están representados por Ilyocypris wichmanni punctata, Eucandona? sp., Cypridopsis sp. y Ovocytheridea? rionegrensis. Los palinomorfos aparecen como una asociación monoespecífica de colonias de algas del género Pediastrum. Las características litológicas, ubicación estratigráfica y el contenido fosilífero permiten correlacionar estos bancos rojos con los de la Formación Ciénaga del Río Huaco. La citada correlación amplía considerablemente la distribución regional de las sedimentitas cretácicas anteriormente limitadas a la Precordillera de San Juan.

  9. Middle and Late Devonian brachiopod assemblages,sea level change and paleogeography of Hunan,China

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Givetian through middle Famennian brachiopods of Hunan are used to define five biozones,comprising,in ascending order,the Stringocephalus Abundance Zone,the Ambocoeliid-Leiorhynchid Assemblage Zone,the Cyrtospiriferid Assemblage Zone,the Yunnanellina-Sinospirifer Assemblage Zone,and the Yunnanella-Hunanospirifer Assemblage Zone.They correspond approximately to the Po.hemiansatus Zone to Po.varcus Zone,S.hermanni Zone to Pa.punctata Zone,Pa.hassi Zone to Pa.linguiformis Zone,Pa.triangularis Zone to Upper Pa.crepida Zone,and Uppermost Pa.crepida Zone to Pa.marginifera Zone,respectively.Intensified rifting activity in South China during the Devonian began in the middle of the Lower Po.varcus Zone(a level marked by the first appearance of Po.rhenanus),which is consistent with the Lower-Middle Givetian substage boundary suggested by the International Subcommission on Devonian Stratigraphy(SDS).This assumed that rifting activity in the Lower Po.varcus Zone was signified by the deposition of the Baqi Formation in Guangxi and was marked largely by the extensive deposition of the Qiziqiao Formation in central Hunan,which probably led to an opposite pattern of relative sea level change-rising in deeper water trough areas and lowering in carbonate platform areas.The previously recognized S.hermanni Zone sea level rise in platform margin areas of Guangxi can be identified not only in the trough areas but also in platform areas,which probably indicates an overall marine transgression at this time,rather than tectonically induced sea level change.Similarly,this level coincides with the Middle-Upper Givetian substage boundary suggested by the SDS.Although the sea level rise may have occurred in the deeper water trough areas of South China towards the end of the Givetian,platformal areas experienced uplifting at various degrees,especially in the central and northern regions of Hunan where Early Frasnian deposits were apparently absent in many places.Similarly,the end

  10. Preliminary examination of food web structure of Nicola Lake (Taim Hydrological System, south Brazil using dual C and N stable isotope analyses

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    Alexandre M. Garcia

    2006-06-01

    Full Text Available Taim Ecological Reserve is located within the Taim Hydrological System and was created to protect a heterogeneous and productive landscape harboring exceptional biological diversity in southern Brazil. Using stable isotope ratio analyses of carbon (delta13C and nitrogen (delta15N, we provide a preliminary description of the food web structure, including estimates of production sources supporting fish populations and vertical trophic structure, within a representative lake of this system. A total of 21 organisms (5 macrophytes, 3 mollusks and 13 adult fishes representing 16 species were collected for isotope analysis. Fishes had delta13C values ranging from -24.30º/oo to -28.31º/oo , showing concordance with the range of values observed for macrophytes (-25.49 to -27.10º/oo, and suggesting that these plants could be a major carbon source supporting these fishes. delta13C signatures of Corbicula (-30.81º/oo and Pomacea (-24.26º/oo indirectly suggest that phytoplankton and benthic algae could be alternative carbon sources for some consumers. Nitrogen isotope ratios indicated approximately three consumer trophic levels. The pearl cichlid Geophagus brasiliensis was a primary consumer. Two catfishes (Trachelyopterus lucenai and Loricariichthys anus were secondary consumers. Two congeneric pike cichclids (Crenicichla lepidota and C. punctata, a catfish (Pimelodus maculatus and the characids Astyanax fasciatus and Oligosarcus robustus were tertiary consumers. Further studies including additional primary producers and consumers and greater sample numbers should be conducted to provide a more complete and detailed description of food web structure and dynamics within the reserve.A Estação Ecológica do Taim está inserida dentro do Sistema Hidrológico do Taim e foi criada para proteger uma região heterogênea e produtiva no sul do Brasil, abrigando uma diversidade biológica excepcional. A partir da análise de isótopos estáveis do carbono

  11. The spider family Micropholcommatidae (Arachnida: Araneae: Araneoidea: a relimitation and revision at the generic level

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    Michael Rix

    2010-02-01

    .; the 20 previously described species of Textricella are thus transferred into Eterosonycha or other newly described genera. The Textricellini includes 10 genera from Australasia and Chile: Eterosonycha has four species, including the type E. alpina Butler (=Textricella parva Hickman syn. n., E. complexa (Forster, E. aquilina sp. n. and E. ocellata sp. n.; Epigastrina gen. n. has three species, including the type E. fulva (Hickman, E. loongana sp. n. and E. typhlops sp. n.; Guiniella gen. n. is monotypic, with G. tropica (Forster; Raveniella gen. n. has three species, including the type R. luteola (Hickman, R. hickmani (Forster and R. peckorum sp. n.; Rayforstia gen. n. has 12 species, including the type R. vulgaris (Forster, the two new species R. lordhowensis sp. n. and R. raveni sp. n., and the nine additional species R. antipoda (Forster, R. insula (Forster, R. mcfarlanei (Forster, R. plebeia (Forster, R. propinqua (Forster, R. salmoni (Forster, R. scuta (Forster, R. signata (Forster and R. wisei (Forster; Normplatnicka gen. n. has three species, including the type N. lamingtonensis (Forster, N. chilensis sp. n. and N. barrettae sp. n.; Eperiella gen. n. has two species, including the type E. alsophila sp. n., and E. hastings sp. n.; Algidiella gen. n. is monotypic, with A. aucklandica (Forster; Taliniella gen. n. has two species, including the type T. nigra (Forster, and T. vinki sp. n.; and Tinytrella gen. n. is monotypic, with T. pusilla (Forster. The micropholcommatine tribe Patelliellini trib. n. includes only one monotypic genus, Patelliella gen. n., represented by the enigmatic species Patelliella adusta sp. n. from Lord Howe Island. The subfamily Taphiassinae subfam. n. includes two genera of distinctive, heavily punctate Micropholcommatidae from Australasia: Taphiassa Simon has six species, including the type T. impressa Simon, T. punctata (Forster, T. castanea sp. n., T. globosa sp. n., T. magna sp. n. and T. robertsi sp. n.; the genus Parapua Forster

  12. The Orchidaceae of Ruiz & Pavón’s “Flora Peruviana et Chilensis”. A taxonomic study. I

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    Pupulin, Franco

    2012-06-01

    , and M. Triphylla are formally lectotypified with the type illustrations conserved in MA. New combinations are proposed for the basionyms Bletia parviflora, Fernandezia punctata, Humboldtia po lystachya, Maxillaria ramosa, and M. Triphylla.

    Bajo el impulso del rey Carlos III, el gobierno español demostró en la segunda mitad del siglo XVIII un serio interés en el estudio de las ricas floras de sus colonias en el Nuevo Mundo. La Real Expedición al Perú y Chile, liderada por Ruiz y Pavón, trabajó por 11 años en las dos colonias y fue continuada posteriormente por Tafalla y Manzanilla, quienes botanizaron en el Perú y en el Ecuador. En 1794 y 1798 se publicaron dos trabajos preliminares sobre la flora del Perú, pero solamente tres de los 11 volúmenes y cinco suplementos de la Flora Peruviana et Chilensis vieron finalmente la luz. El séptimo volumen, dedicado a las Orquídeas, nunca se publicó. Debido a las descripciones sintéticas publicadas en 1798 y a la dispersión de una parte cuantiosa del herbario de Ruiz y Pavón, muchos conceptos de sus especies de orquídeas quedaron obscuros para los botánicos modernos. El estudio crítico de las ilustraciones y manuscritos inéditos de la expedición, conservados en el Real Jardín Botánico de Madrid (RJB, permiten ahora identificar por primera vez muchas de estas especies. Los resultados orquideológicos de la expedición, con una discusión de los nuevos hallazgos e interpretaciones hechos posibles por el estudio de la iconografía de orquídeas de Ruiz y Pavón, se presentan en dos contribuciones. En esta primera parte se proporcionan una introducción, notas sobres los ilustradores y su obra, así como sobre los manuscritos y colecciones de orquídeas. Los táxones de orquídeas se presentan alfabéticamente, de Acianthera a Maxillariella. Para cada uno de los táxones se proveen referencias a los tipos nomenclaturales, sinonimia, ilustraciones y exsiccata preparados

  13. Helminth fauna of bovines from the Central-Western region, Minas Gerais State, Brazil Fauna helmintológica de bovinos da região Centro-oeste do Estado de Minas Gerais, Brasil

    Directory of Open Access Journals (Sweden)

    Thaís Rabelo dos Santos

    2010-04-01

    Full Text Available Seventy-six naturally infected bovines, males and females of mixed breed, aged 8 to 12 months-old, were necropsied. The results of necropsy revealed the presence of 9 helminth genera and 16 species, with the following prevalence and mean infection intensity: Haemonchus placei (100.0%; 3895.5; Haemonchus similis (29.0%; 159.6; Cooperia punctata (100.0%; 5595.0; Cooperia spatulata (32.9%; 137.8; Cooperia pectinata (34.2%; 1010.5; Trichostrongylus axei (69.7%; 239.2; Trichostrongylus colubriformis (10.5%; 10.8; Trichostrongylus longyspicularis (2.6%; 0.5; Ostertagia ostertagi (2.6%; 3.1; Ostertagia lyrata (2.6%; 1.5; Ostertagia trifurcata (1.3%; 0.3; Oesophagostomum radiatum (94.7%; 470.9; Trichuris discolor (47.4%; 32.5; Strongyloides papillosus (1.3%; 0.1; Capillaria bovis (9.2%; 1.0 and Bunostomum phlebotomum (2.6%; 0.3. The mean parasitic load was 11,558.5 helminths per bovine. Of the 76 necropsied bovine, 92.1% were infected by 3 to 7 helminth species. Only 7.9% of hosts were parasitized by 8 different helminth species. This study includes the first report of the species Ostertagia lyrata and Ostertagia trifurcata in Minas Gerais state. It should be emphasized that while identifying the helminths collected during necropsy in the present work, observation revealed that an inversion in the mean parasitic intensity is occurring, showing diminishing numbers of Cooperia and an increase in Haemonchus compared to the values reported in the literature.A prevalência e a intensidade de parasitismo por diferentes espécies de helmintos foram estudadas em bovinos da microrregião de Formiga, região Centro-oeste de Minas Gerais. Para tanto, foram necropsiados 76 bovinos naturalmente infectados, machos e fêmeas, SRD (sem raça definida e de oito a 12 meses de idade. Os resultados necroscópicos revelaram a presença de nove gêneros e 16 espécies de helmintos, com a seguinte prevalência e média de intensidade de infecção: Haemonchus placei (100

  14. Defeitos congênitos diagnosticados em ruminantes na Região Sul do Rio Grande do Sul Congenital defects in ruminants in southern Brazil.

    Directory of Open Access Journals (Sweden)

    Clairton Marcolongo-Pereira

    2010-10-01

    ádicos. Em bubalinos todas as malformações diagnosticadas são hereditárias (artrogripose, miotonia e dermatose mecânico-bolhosa ou suspeitas de serem hereditárias (albinismo, megaesôfago e hidranencefalia/hipoplasia cerebelar. Concluiu-se que os defeitos congênitos esporádicos têm pouca importância nas três espécies e que defeitos congênitos de causas ambientais, apesar de pouco freqüentes, podem trazer prejuízos econômicos importantes em determinadas regiões ou estabelecimentos. As doenças hereditárias são importantes não só pela mortalidade mas, também, pela possibilidade de disseminação de genes indesejáveis nas diferentes raças. Em bubalinos a alta frequência de doenças hereditárias na raça Murrah foi atribuída a alta consanguinidade do rebanho brasileiro. Medidas de controle devem ser tomadas para evitar-se a contínua disseminação, principalmente dos genes recessivos, em bubalinos e bovinos.Congenital defects in cattle, sheep and buffalo were studied through a review of necropsy files of the Regional Diagnostic Laboratory of the Veterinary Faculty at the Federal University of Pelotas between 1978 and 2009. The occurrence of congenital defects in cattle, sheep and buffalo were 0.88%, 0.36%, and 7.54%, respectively, from all specimens received. Cattle congenital defects of undetermined etiology represented 45.83% of the congenital defects, known hereditary and probably hereditary diseases represented 6.25%, and 29.16%, respectively, and defects associated to environmental factors represented 16.66%. In cattle, of the 48 congenital defects observed 21 (43.75% affected the skeletal system (chondrodysplasia, scoliosis, lateral deviation of the mandible, palatoschisis and unclassified defect, nine (18.75% affected the central nervous system (hypoplasia of olfatory and frontal lobes, cerebellar cortical degeneration, spina bifida, congenital hypomielinogenesis, hereditary hypermetria, cerebellar hypoplasia, and pachygiria, nine (18.75% the

  15. Phytoplankton Diversity of Hongqi Reservoir in Jimunai%新疆吉木乃红旗水库浮游植物多样性研究

    Institute of Scientific and Technical Information of China (English)

    吴惠仙; 王琼; 蔡桢; 陈文银; 黄硕琳; 朱新英; 李周永; 薛俊增

    2011-01-01

    Hongqi Reservoir is supplied with snow melt. It locates in Jimunai ,Xinjiang. Phytoplankton community structure was investigated and analyzed to explain the ecological characters of Hongqi Reservoir on July 2008, October 2008 and May 2009. A total of 113 phytoplankton species, belonging to 8 phylum were identified. There were 42 species of diatom,38 species of green algae, 10 species of euglenids,9 species of blue green algae,4 species of yellow green algae,4 species of chrysophyceae,3 species of fire algae,2 species of cryptomonads. Green algae and diatoms was dominance in the species composition. Merismopedia punctata,Phormidium tenus ,Botrydiopsis arhiza,Cyclotella bodanica,Chlorella vulgaris ,Schroederia nitzschioides as well as Ankistrodesmus acicularis are the dominant species. Phytoplankton species composition in different periods showed significant differences (P<0.01),the wet season (57 species)>the normal-water season (42 species)>the dry season (31 species). Chlorophyta has the largest cell density, but Pyrrophyta and Cryptophyta were the lowest. And cell density between different categories of phytoplankton was significantly difference (P< 0.001 ). The cell density in different periods was also significant difference(P<0.01 ), the cell density of the normal-water season was(5.03±6.40)×l05 ind/L,the wet season was(2.40± 4.98)×l05 ind/L,the dry season was(0.37±0.42)×l05 ind/L. Moreover,that the phytoplankton composition,cell density and diversity were closely related to the water temperature of Hongqi Reservoir.%红旗水库位于新疆阿勒泰地区吉木乃县,为了解其浮游植物生态现状,于2008年7月(平水-期)、10月(枯水期)和次年5月(丰水期)对红旗水库浮游植物群落生态特征进行了研究.共采集浮游植物8门113种,其中硅藻门42种,绿藻门38种,裸藻门10种,蓝藻门9种,金藻门5种,黄藻门4种,甲藻门3种,隐藻门2种,在种类组成上具有显著优势的是绿藻门与硅藻

  16. Comparative efficacy of ivermectin pour-on, albendazole, oxfendazole and fenbendazole against Ostertagia ostertagi inhibited larvae, other gastrointestinal nematodes and lungworm of cattle.

    Science.gov (United States)

    Williams, J C; DeRosa, A; Nakamura, Y; Loyacano, A F

    1997-12-15

    An experiment was conducted to evaluate the current efficacy of albendazole (ABZ), oxfendazole (OXF) and fenbendazole (FBZ) compared with ivermectin pour-on (IVM-PO) against inhibited early fourth-stage larvae (IEL4) of Ostertagia ostertagi, other gastrointestinal nematodes and lungworm of cattle during spring in Louisiana. Twenty-five crossbred beef heifer calves of 235 kg average weight and 10-12 months of age were acquired in late winter and grazed for 9 weeks on pasture contaminated with O. ostertagi and other nematodes until May 15. The cattle were weighed and randomly allotted into 5 groups of 5 calves on May 16 (day 0) and treatments were as follows: group 1, nontreated controls (CONT); group 2, IVM-PO on mid-backline at 500 micrograms/kg; group 3, ABZ suspension (oral) at 10 mg/kg; group 4, OXF suspension (oral) at 4.5 mg/kg; group 5, FBZ suspension (oral) at 5 mg/kg. After treatment and confinement in separate pens for each group, approximately equal numbers of cattle from each group were necropsied daily between days 29-31. Mean numbers of O. ostertagi developmental stages present in CONT were: adult, 5234; developing (DL4), 3130; IEL4, 44,077. The mean percentage of IEL4 was 84.1. Cooperia spp. were the second most prevalent in CONT (20,307) and smaller numbers of abomasal and intestinal species and Dictyocaulus viviparus were present in nearly all CONT. Percent reductions for the four compounds against O. ostertagi adult, DL4 and IEL4, respectively, were IVM-PO: 99.7, 98.3, 98.1; ABZ: 74.1, 76.5, 75.3; OXF: 78.5, 42.1, 32.0; FBZ: 63.6, 17.7, 39.7. Efficacy of IVM-PO was greater (P < 0.05) against all O. ostertagi stages than the benzimidazole (BZ) drugs, except for ABZ (DL4). There were no significant differences in group means (except for C. punctata adult males, P < 0.05 lower for IVM-PO) or wide variation in reduction percentages for other abomasal and intestinal species and D. viviparus between IVM-PO and BZ drugs. The low efficacy of all three BZ

  17. Preliminary study on screening of cadmium hyperaccumulator duckweed strain and removal of cadmium in water%重金属镉超富集浮萍品种筛选及其对水体中镉的去除效果

    Institute of Scientific and Technical Information of China (English)

    唐利萍; 方扬; 靳艳玲; 陈夏媛; 赵海

    2015-01-01

    为筛选出能高效去除水体重金属镉,并能快速积累生物量的浮萍品种,对实验室保存的12个能耐受30 mg/L镉浓度的浮萍品种在0.5 mg/L和10mg/L的镉浓度下进行复筛,获得最优品种少根紫萍(Landoltia punctata)ZH0049,并研究其在不同镉浓度胁迫下的生长状况和镉富集能力,同时分析其叶绿素含量变化与镉胁迫浓度的关系.结果显示:ZH0049在0-0.5mg/L的镉浓度范围内能正常生长,干物质积累率最高可达到6.44 g m2d-1.在0-3 mg/L的镉浓度范围内,ZH0049对镉的吸收率、去除率以及富集系数在0.5 mg/L时出现波谷,而在3mg/L时达到最高,分别为66.74%、72.43%和770.叶绿素相对含量实验结果显示,当镉浓度大于0.5 mg/L,浮萍生长受到抑制,叶绿素a、b含量开始下降,相对于初始值最高下降了61.79%和32.08%.当镉浓度从0.5 mg/L至3 mg/L,叶绿素相对含量(Chl a/Chl b)相对于初始值分别下降了3.49%、7.28%、19.32%、31.33%和42.59%,表明叶绿素a降幅大于叶绿素b.综上,ZH0049能将水体中的镉富集在体内的同时,保持较高的干物质积累量,从而达到较好的重金属去除效果,为水体中镉的去除建立了一种新途径.

  18. Helminth parasites of cattle from Jaboticabal, São Paulo State, Brazil/ Fauna helmintológica de bovinos da região de Jaboticabal, Estado de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Alvimar José da Costa

    2001-08-01

    Full Text Available This paper deals with the study of parasites in cattle in the region of Jaboticabal, São Paulo. For this, 42 holstein-zebu mixed breeding animals, 8 to 14 month old, were necropsied. At necropsy 17 species of helminth were collected. The prevalence and intensity of infection were as follow: Haemonchus placei 97,62% (1961,81; Cooperia punctata 92,86% (8109,45; Oesophagostomum radiatum 73,81% (217,38; Trichuris discolor 38,19% (25,48; Trichostrongylus axei 26,19% (122,05; H. simillis 21,43% (37, 45; C. pectinata 19,05% (198,6; Bunostomum phlebotomum 16,66% (8,95; Dictyocaulus viviparus 16,66% (3,62; C. spatulata14,29% (109,95; Capillaria bovis 11,90% (5,48; Ostertagia ostertagi 7,14% (0,86; O. lyrata 4,76% (0,17; Eurytrema coelomaticum 4,76% (2,14; Moniezia benedeni, 4.76% (0,05; T. colubriformis 2,38% (0,48; Strongyloides papillosus 2,38% (0,25. Each calf had an average worm burden of 10804 helminths Haemonchus (18,5% and Cooperia (77,91% were the most frequently observed helminths. Mixed infections were commonly observed, 71,43% of the animals were parasitized with three to five different species of helminths. Only 2,38% of the calves were parasitized with ten different species of helminths.Foram colhidas 600 amostras de soros de vacas, pertencentes a propriedades rurais dos municípios de Pirangi, Votuporanga, Guaíra e Ituverava, Estado de São Paulo e dos municípios de Iturama e Gurinhatã, Estado de Minas Gerais. As amostras foram analisadas pela técnica de imunofluorescência indireta (RIFI-IgG, para a pesquisa de anticorpos contra Neospora caninum e contra Toxoplasma gondii. Os exames sorológicos revelaram 16,83% e 49,17% de animais sororreagentes ao N. caninum e ao T. gondii, respectivamente. Foram encontrados 54 (9,00% animais apresentando títulos de anticorpos para ambos coccídios. Em todos os municípios analisados foi detectada a presença de animais sororreagentes. Diferenças estatísticas (P < 0,05 foram constatadas nos

  19. 本体觉传入纤维在小鼠脊髓内的发育变化%DEVELOPMENTAL ALTERNATIONS IN PROPRIOCEPTIVE AFFERENT PROJECTIONS IN THE MOUSE SPINAL CORD

    Institute of Scientific and Technical Information of China (English)

    黄静; 冯枫; 刘翔宇; 李云庆; 武胜昔

    2006-01-01

    目的观察本体觉传入纤维在小鼠脊髓内投射和终止的发育变化. 方法采用小牛白蛋白(PV)免疫组织化学染色特异标记本体觉传入纤维,用免疫荧光单标记和双标记方法观察本体觉传入纤维在脊髓内的生长模式以及与运动神经元的关系.染色后的切片用激光共聚焦显微镜进行观察. 结果PV样免疫阳性(LI)本体觉纤维最早于胚胎(E)14 d出现在后索,E15时进入脊髓灰质.E16时,已有较多的PV-LI纤维到达中间带灰质和前角(VH).此后,随着发育阶段的增长,脊髓VH内PV-LI本体觉纤维和终末的数量和密度逐渐增加,并在生后早期P0-P7达到最高水平.P14后,上述本体觉纤维和终末的数量逐渐减少.本体觉传入纤维的终末在E17时开始与脊髓VH运动神经元形成密切的接触.结论本体觉传入纤维在脊髓内的定位模式形成于小鼠胚胎后期和生后早期,本研究结果为深入理解脊髓反射运动环路的发育特点提供了依据.%Objective To observe the developmental changes of projection and termination of proprioceptive afferent fibers in the mouse spinal cord. Methods Parvalbumin (PV) immunohistochemistry was used to label the proprioceptive afferents. Single and dual immunofluorescence histochemistry were used to examine the growth pattern of proprioceptive afferents and their relationships with motoneurons in the spinal ventral horn (VH). The stained sections were observed under a confocal laserscanning microscope. Results PV-like immunoreactive (LI) proprioceptive fibers first appeared in the dorsal column on embryonic (E) day 14, then entered the gray matter on El5 and reached the intermediate gray matter and VH more obviously on E16. The number and intensity of PV-LI proprioceptive afferent fibers and punctata increased in the VH with age and reached a maximum during earlier postnatal (P) period (P0-P7). After P14, the number and intensity of proprioceptive afferents gradually

  20. The Community Characteristics of Planktonic Microalgae in Tilapai's Culture Ponds Near the Pearl River Estuary%珠江河口区罗非鱼养殖池塘中浮游微藻的群落特征

    Institute of Scientific and Technical Information of China (English)

    彭聪聪; 李卓佳; 曹煜成; 文国樑; 刘孝竹; 胡晓娟

    2012-01-01

    The continual and regular surveys on the planktonic microalgae community were conducted in six Tilapia's culture ponds in Panyu District, Guangzhou, Guangdong Province from May to November, 2009. The results showed that a total of 157 planktonic microalgae species were identified during the culture period. Among them, 67 species were Chlorophyta, 34 were Cyanophyta, 18 were Bacillariophyta, 5 were Dinophyta, 3 were Cryptophyta, 28 were Euglenophyta, 2 were Chrysophyta. The dominant species in every ponds were rich but composition was complex, and there were large differences among the cell volume of the different dominant species. The dominant species which take the advantage of the microalgae's density were Oscillatoria willei, Merisnwpedia punctata, Nostoc fuscescens, Gomphosphaeria oponina, Micro-cystis firma, Microcystis incerta, Chlorella pyrenoidosa, Ankistrodesmus falcatus, Coelastrum sphaericum and so on. Moreover, the dominant species which just take the advantage of the microalgae's biomass were Euglenapisciformis, Lepocinclis autumnalis, Phacus platalea, Phacus tortus, Strombonwnas schauinslandii, Peridinium gatunense, Gonyaulax polygramma, Cryptomonas ovata, Chroomonas candata and so on. Spirulina spirulinoides was not only the dominant species in number but also in biomass. In the early and middle period, the microalgae density and biomass increased rapidly. Moreover, the microalgae diversity was rich and the community was stable. But in the latter, most of them were often lower. During the early and middle culture period, the range of the quantities of microalgae, the biomass and the diversity index average were(0.59~72.85)×l07 ind·L-1, 1.12~114.14 mg·L-1, and 2.98, respectively. However, in the final phase, the microalgae density, the biomass and the diversity index average ranged from( 16.29~67.37)×l07 ind·L-1, 6.57~67.76 mg·L-1, and 2.78. The microalgae density and biomass in ponds usually appeared significant positive correlation