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Sample records for chino vigna unguiculata

  1. Improvement of the Chinese bean [Vigna Unguiculata (L.) Walp.], through radioinduced mutagenesis; Mejoramiento de Frijol Chino [Vigna Unguiculata (L.) Walp.], Mediante Mutagenesis Radioinducida

    Energy Technology Data Exchange (ETDEWEB)

    Salmeron E, J.; Bueno J, J.E.; Valencia E, F.; Solis M, M. [Colegio Superior Agropecuario del Estado de Guerrero, Iguala (Mexico); Cervantes S, T. [Instituto de Recursos Geneticos y Productividad (Mexico); Cruz T, E. de la [ININ, Carretera Mexico-La Marquesa S/N, La Marquesa Ocoyoacac, Mexico. C.P. 52750 (Mexico)]. e-mail: csaegro@prodigy.net.mx

    2006-07-01

    The advances in the process of genetic improvement of the Chinese bean (Vigna Unguiculata (L.) are presented, high nutritious value that it is evaluating as alternative for marginal areas producers of the State of Guerrero. The method of improvement applied it is recurrent radiation, continued by selection cycles applying the method of progeny by plant. The applied radiation doses were 200 and 250 Gray. The established selection approaches are: resistant plants or tolerant to the plagues attack and illnesses, vigorous, with more height to the first sheath, of compact and certain growth, with short internodes, bigger number of sheaths by plant and of grains by sheath, bigger number of grain size, among others. The obtained results show that the dose that induces bigger variability and that it has propitiated the biggest quantity in possible mutants it is 200Gy. Precocious plants with more height to the first sheath, with certain growth as well as with bigger number and sheaths size have been detected. The selected plants have incorporated to an increment process by means of the progeny method by plant. (Author)

  2. PHYTOCHEMICAL PROPERTIES AND ANTHELMINTIC ACTIVITY OF VIGNA UNGUICULATA LINN.

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    Maisale A B

    2012-04-01

    Full Text Available Vigna unguiculata Linn belonging to family Fabaceae are used traditionally as appetizer, diuretic, laxative, anthelmintic. Seeds are coarse powdered and exhaustively with hot solvent (Soxhlet extraction by ethanol and maceration with chloroform water I.P. Five concentrations (10-100 mg/ml of ethanolic and aqueous extracts were studied for anthelmentic activity by using Eudrilus euginiae earthworms. Both aqueous and ethanolic extracts showed paralysis and death of worms in concentration (10-100 mg/ml dependent manner. Alcoholic extract of Vigna unguiculata showed significant activity than aqueous extract. Piperazine citrate (10 mg/ml and distilled water were included in the assay as standard drug and control respectively. The result showed seeds of vigna unguiculata possessed potential anthelmintic activity. The seeds extract also showed presence of flavonoids, and glycosides by preliminary phytochemical investigations.

  3. Regeneration and genetic transformation of cowpea (Vigna unguiculata Walp.)

    International Nuclear Information System (INIS)

    Regeneration of cowpea (Vigna unguiculata Walp.) was achieved through massive bud formation induced in apical and lateral meristems by the herbicide Thidiazuron (TDZ). The effect of TDZ (5, 10, or 20 μM) was tested in vitro on four different cowpea genotypes. Thidiazuron, even at the highest concentration, had no effect on seed germination. After one month of culture, multiple bud cluster formation was observed in all genotypes tested; about 80% of shoot apices regenerated multiple buds, whilst only 34% of cotyledonary nodes behaved in the same way. Histology of regenerating multiple bud clusters revealed that regeneration initiated from pre-existing meristems in the apex and cotyledonary node. Thidiazuron at 10 μM appeared to be the best concentration to produce clusters with high number of buds, ranging from 5 to 10. Shoot elongation occurred only on MS medium without TDZ. On the same medium, 75% of elongated shoots rooted. For genetic transformation of cowpea, a direct DNA transfer methods in plants under in vivo conditions was tested by electroporation of plasmid DNA into the nodal meristematic cells. Some transformed plants were obtained, and produced T1 transformed progenies; their transgenic nature was confirmed by Southern analysis. (author). 21 refs, 2 figs, 3 tabs

  4. Analysis of radiation-induced genome alterations in Vigna unguiculata

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    van der Vyver C

    2011-09-01

    Full Text Available Christell van der Vyver1, B Juan Vorster2, Karl J Kunert3, Christopher A Cullis41Institute for Plant Biotechnology, Department of Genetics, University of Stellenbosch, Stellenbosch, South Africa; 2Department of Plant Production and Soil Science, and 3Department of Plant Science, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South Africa; 4Case Western Reserve University, Department of Biology, Cleveland, OH, USAAbstract: Seeds from an inbred Vigna unguiculata (cowpea cultivar were gamma-irradiated with a dose of 180 Gy in order to identify and characterize possible mutations. Three techniques, ie, random amplified polymorphic DNA, microsatellites, and representational difference analysis, were used to characterize possible DNA variation among the mutants and nonirradiated control plants both immediately after irradiation and in subsequent generations. A large portion of putative radiation-induced genome changes had significant similarities to chloroplast sequences. The frequency of mutation at three of these isolated polymorphic regions with chloroplast similarity was further determined by polymerase chain reaction screening using a large number of individual parental, M1, and M2 plants. Analysis of these sequences indicated that the rate at which various regions of the genome is mutated in irradiation experiments differs significantly and also that mutations have variable “repair” rates. Furthermore, regions of the nuclear DNA derived from the chloroplast genome are highly susceptible to modification by radiation treatment. Overall, data have provided detailed information on the effects of gamma irradiation on the cowpea genome and about the ability of the plant to repair these genome changes in subsequent plant generations.Keywords: mutation breeding, gamma radiation, genetic mutations, cowpea, representational difference analysis

  5. Identification of markers associated with bacterial blight resistance loci in cowpea (Vigna unguiculata (L.) Walp.)

    NARCIS (Netherlands)

    Agbicodo, A.C.M.E.; Fatokun, C.A.; Bandyopadhyay, R.; Wydra, K.; Diop, N.N.; Muchero, W.; Ehlers, J.D.; Roberts, P.A.; Close, T.J.; Visser, R.G.F.; Linden, van der C.G.

    2010-01-01

    Cowpea bacterial blight (CoBB), caused by Xanthomonas axonopodis pv. vignicola (Xav), is a worldwide major disease of cowpea [Vigna unguiculata (L.) Walp.]. Among different strategies to control the disease including cultural practices, intercropping, application of chemicals, and sowing pathogen-fr

  6. Genetic analysis of abiotic and biotic resistance in cowpea [Vigna unguiculata (L.) Walp.

    NARCIS (Netherlands)

    Agbicodo, A.C.M.E.

    2009-01-01

    Cowpea [Vigna unguiculata (L.) Walp.] is a most versatile African crop, it feeds people, their livestock and because of its ability in nitrogen-fixation, it improves soil fertility, and consequently helps to increase the yields of cereal crops when grown in rotation and contributes to the sustainabi

  7. Nitrogen assimilation by nodulate plants of Phaseolus vulgaris l. and Vigna unguiculata (l.) walp

    International Nuclear Information System (INIS)

    Under field conditions, the processes of nitrogen assimilation via nitrogenase and nitrate-reductase, the transport and the accumulation of nitrogen in nodulated plants of Phaseolus vulgaris cv. Rio Tibagi and Vigna unguiculata cv. Vita 34 were compared and contrasted. V. unguiculata showed better nodulation than P. vulgaris and consequently had higher rates of nitrogenase activity. The small nodulation of P. vulgaris resulted in greater dependence on soil mineral nitrogen as indicated by the higher rates of nitrate-reductase acitivty compared with V. unguiculata, especially during reproductive stage of growth. The superiority of V. unguiculata in terms of assimilation and remobilization of stored nitrogen resulted in a seed yield 28% greater than that of P. vulgaris. P. vulgaris showed a negative correlation between the nitrate-reductase activity and the ureide content of the sap indicating that the metabolic pathways leading to ureide production operates alternatively to nitrate assimilation. (Author)

  8. Nitrogen assimilation by nodulate plants of Phaseolus vulgaris l. and Vigna unguiculata (l. ) walp

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    Neves, M.C.P.; Fernandes, M.S.; Sa, M.F.M. (Universidade Federal Rural do Rio de Janeiro (Brazil). Dept. de Solos)

    1982-05-01

    Under field conditions, the processes of nitrogen assimilation via nitrogenase and nitrate-reductase, the transport and the accumulation of nitrogen in nodulated plants of Phaseolus vulgaris cv. Rio Tibagi and Vigna unguiculata cv. Vita 34 were compared and contrasted. V. unguiculata showed better nodulation than P. vulgaris and consequently had higher rates of nitrogenase activity. The small nodulation of P. vulgaris resulted in greater dependence on soil mineral nitrogen as indicated by the higher rates of nitrate-reductase acitivty compared with V. unguiculata, especially during reproductive stage of growth. The superiority of V. unguiculata in terms of assimilation and remobilization of stored nitrogen resulted in a seed yield 28% greater than that of P. vulgaris. P. vulgaris showed a negative correlation between the nitrate-reductase activity and the ureide content of the sap indicating that the metabolic pathways leading to ureide production operates alternatively to nitrate assimilation.

  9. First report of Cowpea mild mottle Carlavirus on yardlong bean (Vigna unguiculata subsp. sesquipedalis) in Venezuela.

    Science.gov (United States)

    Brito, Miriam; Fernández-Rodríguez, Thaly; Garrido, Mario José; Mejías, Alexander; Romano, Mirtha; Marys, Edgloris

    2012-12-14

    Yardlong bean (Vigna unguiculata subsp. sesquipedalis) plants with virus-like systemic mottling and leaf distortion were observed in both experimental and commercial fields in Aragua State, Venezuela. Symptomatic leaves were shown to contain carlavirus-like particles. RT-PCR analysis with carlavirus-specific primers was positive in all tested samples. Nucleotide sequences of the obtained amplicons showed 84%-74% similarity to corresponding sequences of Cowpea mild mottle virus (CPMMV) isolates deposited in the GenBank database. This is the first report of CPMMV in Venezuela and is thought to be the first report of CPMMV infecting yardlong bean.

  10. First Report of Cowpea Mild Mottle Carlavirus on Yardlong Bean (Vigna unguiculata subsp. sesquipedalis in Venezuela

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    Edgloris Marys

    2012-12-01

    Full Text Available Yardlong bean (Vigna unguiculata subsp. sesquipedalis plants with virus-like systemic mottling and leaf distortion were observed in both experimental and commercial fields in Aragua State, Venezuela. Symptomatic leaves were shown to contain carlavirus-like particles. RT-PCR analysis with carlavirus-specific primers was positive in all tested samples. Nucleotide sequences of the obtained amplicons showed 84%–74% similarity to corresponding sequences of Cowpea mild mottle virus (CPMMV isolates deposited in the GenBank database. This is the first report of CPMMV in Venezuela and is thought to be the first report of CPMMV infecting yardlong bean.

  11. TRUE METABOLIZABLE ENERGY AND DIGESTIBILITY OF FIVE Vigna unguiculata VARIETIES IN CHICKENS

    OpenAIRE

    Sarmiento-Franco, L.; E. Gorocica-Pino; L. Ramírez-Avilés; J. Castillo-Caamal; R. Santos-Ricalde; María F. Díaz

    2011-01-01

    This study was carried out to evaluate the effect of heat-treatment on grain true metabolizable energy (TME), dry matter and gross energy digestibilities of five Vigna unguiculata varieties: H82, T782, TM97, C666 y XL. The grain of the former three varieties were heat-treated, and offered raw or cooked, whereas grain of the late two varieties were used only row, resulting in a total of eight treatments. The heat treatment consisted of watering the grains with boiling water for 30 minutes and ...

  12. The resistance of seeds of cowpea (Vigna unguiculata to the cowpea weevil (Callosobruchus maculatus

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    J. Xavier Filho

    1991-01-01

    Full Text Available Cowpea (Vigna unguiculata seeds are heavily damaged during storage by the bruchid Callosobruchus maculatus. Seeds of some Nigerian varieties showed a strong resistance to this bruchid. By utilizing biochemical and entomological techniques we were able to rule out the paticipation of proteolytic enzyme (trypsin, chimotrypsin, subtilisin and papain inhibitors, lectins, and tannins in the resistance mechanisms. Fractionation of the seed meal of a resistant variety suggests that the factor(s responsible for the effect is (are concentrate in the globulin fraction.

  13. Vigna unguiculata is nodulated in Spain by endosymbionts of Genisteae legumes and by a new symbiovar (vignae) of the genus Bradyrhizobium.

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    Bejarano, Ana; Ramírez-Bahena, Martha-Helena; Velázquez, Encarna; Peix, Alvaro

    2014-10-01

    Vigna unguiculata was introduced into Europe from its distribution centre in Africa, and it is currently being cultivated in Mediterranean regions with adequate edapho-climatic conditions where the slow growing rhizobia nodulating this legume have not yet been studied. Previous studies based on rrs gene and ITS region analyses have shown that Bradyrhizobium yuanmingense and B. elkanii nodulated V. unguiculata in Africa, but these two species were not found in this study. Using the same phylogenetic markers it was shown that V. unguiculata, a legume from the tribe Phaseolae, was nodulated in Spain by two species of group I, B. cytisi and B. canariense, which are common endosymbionts of Genisteae in both Europe and Africa. These species have not been found to date in V. unguiculata nodules in its African distribution centres. All strains from Bradyrhizobium group I isolated in Spain belonged to the symbiovar genistearum, which is found at present only in Genisteae legumes in both Africa and Europe. V. unguiculata was also nodulated in Spain by a strain from Bradyrhizobium group II that belonged to a novel symbiovar (vignae). Some African V. unguiculata-nodulating strains also belonged to this proposed new symbiovar. PMID:24867807

  14. Physiological effects of seed coat darkening in Cowpea bean (Vigna unguiculata L. Walp): aging and water uptake

    Science.gov (United States)

    Cowpea bean (Vigna unguiculata L. Walp) is an important annual food crop in Northeast Brazil. Dry storage of these seeds leads to a slow and uneven darkening of the seed coat. The mixture of seed colors creates an unacceptable product for consumers. The aim of this study was to determine the kineti...

  15. Transformation of Cowpea Vigna unguiculata Cells with an Antibiotic Resistance Gene Using a Ti-Plasmid-Derived Vector

    NARCIS (Netherlands)

    Hille, Jacques; Goldbach, Rob

    1986-01-01

    A chimaeric antibiotic resistance gene was transferred to cowpea (Vigna unguiculata), a member of the legume family. This transfer was established by inoculating cowpea leaf discs with an Agrobacterium tumefaciens strain harboring a Ti-plasmid-derived vector that contained two copies of a chimaeric

  16. Effect of Exogenous Application of Silicon with Drought Stress on Protein and Carbohydrate Contents of Edible Beans (Vigna radiate & Vigna unguiculata

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    N. Hamid

    2012-06-01

    Full Text Available An experiment was carried out under field conditions to determine the impact of silicon application with different concentrations (20, 40, 60 ppm, on selected physiological characteristics of the leaves of mungbean (Vignaradiata and cowpea (Vigna unguiculata under different 100%, 75%, 50% and 25% soil moisture regimes. Stock solution (100 ppm of silicon was prepared by MgSi3 salt but apply as 20, 40, 60 ppm solution in both treated and control plants. Results showed that silicon application significantly increases total carbohydrate & protein contents in treated samples as compare to control plants. In present study we concluded that silicon promoted growth in the drought susceptible species to greater extent & it’s more beneficial for carbohydrates and protein metabolism of cowpea (Vigna unguiculata as compare to mungbean (Vignaradiata plants.

  17. Programmed cell death during development of cowpea (Vigna unguiculata (L.) Walp.) seed coat.

    Science.gov (United States)

    Lima, Nathália Bastos; Trindade, Fernanda Gomes; da Cunha, Maura; Oliveira, Antônia Elenir Amâncio; Topping, Jennifer; Lindsey, Keith; Fernandes, Kátia Valevski Sales

    2015-04-01

    The seed coat develops primarily from maternal tissues and comprises multiple cell layers at maturity, providing a metabolically dynamic interface between the developing embryo and the environment during embryogenesis, dormancy and germination of seeds. Seed coat development involves dramatic cellular changes, and the aim of this research was to investigate the role of programmed cell death (PCD) events during the development of seed coats of cowpea [Vigna unguiculata (L.) Walp.]. We demonstrate that cells of the developing cowpea seed coats undergo a programme of autolytic cell death, detected as cellular morphological changes in nuclei, mitochondria, chloroplasts and vacuoles, DNA fragmentation and oligonucleosome accumulation in the cytoplasm, and loss of membrane viability. We show for the first time that classes 6 and 8 caspase-like enzymes are active during seed coat development, and that these activities may be compartmentalized by translocation between vacuoles and cytoplasm during PCD events. PMID:25142352

  18. Measurements of experimental precision for trials with cowpea (Vigna unguiculata L. Walp.) genotypes.

    Science.gov (United States)

    Teodoro, P E; Torres, F E; Santos, A D; Corrêa, A M; Nascimento, M; Barroso, L M A; Ceccon, G

    2016-01-01

    The aim of this study was to evaluate the suitability of statistics as experimental precision degree measures for trials with cowpea (Vigna unguiculata L. Walp.) genotypes. Cowpea genotype yields were evaluated in 29 trials conducted in Brazil between 2005 and 2012. The genotypes were evaluated with a randomized block design with four replications. Ten statistics that were estimated for each trial were compared using descriptive statistics, Pearson correlations, and path analysis. According to the class limits established, selective accuracy and F-test values for genotype, heritability, and the coefficient of determination adequately estimated the degree of experimental precision. Using these statistics, 86.21% of the trials had adequate experimental precision. Selective accuracy and the F-test values for genotype, heritability, and the coefficient of determination were directly related to each other, and were more suitable than the coefficient of variation and the least significant difference (by the Tukey test) to evaluate experimental precision in trials with cowpea genotypes. PMID:27173351

  19. Diversity patterns of indigenous arbuscular mycorrhizal fungi associated with rhizosphere of cowpea (Vigna unguiculata (L.) Walp.) in Benin, West Africa

    OpenAIRE

    Johnson, J. M.; Houngnandan, P.; A. Kane; Sanon, K. B.; Neyra, Marc

    2013-01-01

    Assessment of diversity and understanding factors underlying species distribution are fundamental themes in ecology. However, the diversity of native arbuscular mycorrhizal fungi (AMF) species in African tropical agro-ecosystems remains weakly known. This research was carried out to assess the morphological diversity of indigenous AMF species associated with rhizosphere of cowpea (Vigna unguiculata (L) Walp.) in different agro-ecological zones (AEZ) of Benin and to examine the effects of soil...

  20. A SNP and SSR based genetic map of asparagus bean (Vigna. unguiculata ssp. sesquipedialis and comparison with the broader species.

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    Pei Xu

    Full Text Available Asparagus bean (Vigna. unguiculata ssp. sesquipedialis is a distinctive subspecies of cowpea [Vigna. unguiculata (L. Walp.] that apparently originated in East Asia and is characterized by extremely long and thin pods and an aggressive climbing growth habit. The crop is widely cultivated throughout Asia for the production of immature pods known as 'long beans' or 'asparagus beans'. While the genome of cowpea ssp. unguiculata has been characterized recently by high-density genetic mapping and partial sequencing, little is known about the genome of asparagus bean. We report here the first genetic map of asparagus bean based on SNP and SSR markers. The current map consists of 375 loci mapped onto 11 linkage groups (LGs, with 191 loci detected by SNP markers and 184 loci by SSR markers. The overall map length is 745 cM, with an average marker distance of 1.98 cM. There are four high marker-density blocks distributed on three LGs and three regions of segregation distortion (SDRs identified on two other LGs, two of which co-locate in chromosomal regions syntenic to SDRs in soybean. Synteny between asparagus bean and the model legume Lotus. japonica was also established. This work provides the basis for mapping and functional analysis of genes/QTLs of particular interest in asparagus bean, as well as for comparative genomics study of cowpea at the subspecies level.

  1. Improvement of the Chinese bean [Vigna Unguiculata (L.) Walp.], through radioinduced mutagenesis

    International Nuclear Information System (INIS)

    The advances in the process of genetic improvement of the Chinese bean (Vigna Unguiculata (L.) are presented, high nutritious value that it is evaluating as alternative for marginal areas producers of the State of Guerrero. The method of improvement applied it is recurrent radiation, continued by selection cycles applying the method of progeny by plant. The applied radiation doses were 200 and 250 Gray. The established selection approaches are: resistant plants or tolerant to the plagues attack and illnesses, vigorous, with more height to the first sheath, of compact and certain growth, with short internodes, bigger number of sheaths by plant and of grains by sheath, bigger number of grain size, among others. The obtained results show that the dose that induces bigger variability and that it has propitiated the biggest quantity in possible mutants it is 200Gy. Precocious plants with more height to the first sheath, with certain growth as well as with bigger number and sheaths size have been detected. The selected plants have incorporated to an increment process by means of the progeny method by plant. (Author)

  2. Prioritising in situ conservation of crop resources: a case study of African cowpea (Vigna unguiculata).

    Science.gov (United States)

    Moray, C; Game, E T; Maxted, N

    2014-06-17

    Conserving crop wild relatives (CWR) is critical for maintaining food security. However, CWR-focused conservation plans are lacking, and are often based on the entire genus, even though only a few taxa are useful for crop improvement. We used taxonomic and geographic prioritisation to identify the best locations for in situ conservation of the most important (priority) CWR, using African cowpea (Vigna unguiculata (L.) Walp.) as a case study. Cowpea is an important crop for subsistence farmers in sub-Saharan Africa, yet its CWR are under-collected, under-conserved and under-utilised in breeding. We identified the most efficient sites to focus in situ cowpea CWR conservation and assessed whether priority CWR would be adequately represented in a genus-based conservation plan. We also investigated whether priority cowpea CWR are likely to be found in existing conservation areas and in areas important for mammal conservation. The genus-based method captured most priority CWR, and the distributions of many priority CWR overlapped with established conservation reserves and targets. These results suggest that priority cowpea CWR can be conserved by building on conservation initiatives established for other species.

  3. EFFECT OF DIETARY LEVELS OF COWPEA (Vigna unguiculata SEEDS ON EGG QUALITY

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    N.G. BALAIEL

    2012-07-01

    Full Text Available The objectives of this study were to evaluate the effect of including 3 dietary levels of grain meal cowpea (Vigna unguiculata 0, 5, 10 and 15% on external and internal egg characteristics. Seventy two laying hen of 28 weeks age were randomly allocated into 4 dietary treatments of 0, 5, 10 and 15% levels of cowpea seeds, which were further replicated 6 times in completely randomized design. Feed and water supply were offered to birds ad libitum while standard management practices were adopted. The results showed that the external parameters like maximum length and width, shell thickness and shell % and egg weight were significantly (P0.05 influenced by dietary treatments. Internal quality characteristics were not significantly (P>0.05 affected by dietary treatments except for albumin weight, albumin percentage, yolk index and yolk colour. It is concluded that commercial egg characteristics such as egg weight is satisfactory maintained with 5 to 1`0% inclusion of cowpea grain meal in balanced diets for layers.

  4. Growth, photosynthesis, and antioxidant responses of Vigna unguiculata L. treated with hydrogen peroxide

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    Syed Aiman Hasan

    2016-12-01

    Full Text Available Cowpea (Vigna unguiculata L. is an important legume well grown in semiarid and arid environment. Hydrogen peroxide solutions (0.1, 0.5, 1.0, and 1.5 mM have been used to optimize growth and photosynthetic performance of cowpea plant at two growth stages [30 and 45 DAS (days of sowing]. Foliar application of H2O2 at 0.5 > 1.0 mM solution at 29 DAS optimally promoted the photosynthetic attributes [leaf chlorophyll content, net photosynthetic rate (PN, water use efficiency, and maximum quantum yield of PSII (Fv/Fm] and growth performance [root and shoot length; fresh and dry weight] of plants where the responses were more significant at the later growth stage. It was favored by activity of enzymes as carbonic anhydrase [CA; E.C. 4.2.1.1] and nitrate reductase [NR, E.C. 1.6.6.1] and those of antioxidant enzymes viz. peroxidase [POX; EC 1.11.1.7], catalase [CAT; EC 1.11.1.6], and superoxide dismutase [SOD; EC 1.15.1.1] and leaf proline content. Strengthened root system and antioxidant activity, particularly leaf proline level appeared to be the key factor for efficient photosynthesis and growth responses.

  5. Effect of gamma irradiation and cooking on cowpea bean grains (Vigna unguiculata L. Walp)

    Energy Technology Data Exchange (ETDEWEB)

    Santos Cople Lima, Keila dos, E-mail: keila@ime.eb.br [Nuclear Engineering Department, Military Institute of Engineering, Rio de Janeiro/RJ, Praca General Tiburcio, 80, CEP 22290-270 Rio de Janeiro/RJ (Brazil); Boher e Souza, Luciana [Nuclear Engineering Department, Military Institute of Engineering, Rio de Janeiro/RJ, Praca General Tiburcio, 80, CEP 22290-270 Rio de Janeiro/RJ (Brazil); Oliveira Godoy, Ronoel Luiz de [Technological Center, Embrapa Food Agroindustry, Av. das Americas, 29501, CEP 23020-470 Rio de Janeiro/RJ (Brazil); Costa Franca, Tanos Celmar; Santos Lima, Antonio Luis dos [Chemical Engineering Department, Military Institute of Engineering, Praca General Tiburcio, 80, CEP 22290-270 Rio de Janeiro/RJ (Brazil)

    2011-09-15

    Leguminous plants are important sources of proteins, vitamins, carbohydrates, fibers and minerals. However, some of their non-nutritive elements can present undesirable side effects like flatulence provoked by the anaerobic fermentation of oligosaccharides, such as raffinose and stachyose, in the gut. A way to avoid this inconvenience, without any change in the nutritional value and post-harvesting losses, is an irradiation process. Here, we evaluated the effects of gamma irradiation on the amino acids, thiamine and oligosaccharide contents and on the fungi and their toxin percentages in cowpea bean (Vigna unguiculata L. Walp) samples. For irradiation doses of 0.0, 0.5, 1.0, 2.5, 5.0 and 10.0 kGy the results showed no significant differences in content for the uncooked samples. However, the combination of irradiation and cooking processes reduced the non-nutritive factors responsible for flatulence. Irradiation also significantly reduced the presence of Aspergillus, Penicilium, Rhizopus and Fusarium fungi and was shown to be efficient in grain conservation for a storage time of 6 months. - Highlights: > In this study we evaluated cowpea beans subjected to different doses of gamma irradiation > Cowpea bean grains represent an important source of vegetal protein for Brazilian population. > Non-nutritive factors were reduced by irradiation and cooking. > Several genera of fungus were reduced by irradiation without affecting the nutritional content. > Irradiation helps the cooking process preserving thermosensible nutrients.

  6. Simplified methods for screening cowpea cultivars for manganese leaf-tissue tolerance. [Vigna unguiculata

    Energy Technology Data Exchange (ETDEWEB)

    Wissemeier, A.H.; Horst, W.J. (Univ. of Hannover (West Germany))

    In cowpea (Vigna unguiculata (L.) Walp.) dark brown speckles on old leaves are typical symptoms of Mn toxicity and indicate Mn sensitivity of leaf tissue. Induction and subsequent quantification of brown Mn speckles in leaf tissues were used to screen cowpea cultivars for Mn leaf-tissue tolerance using three different techniques: (i) leaf cuttings cultured for 22 days in solution culture with 20 {mu}M MnSO{sub 4}, (ii) leaf rings mounted on leaves of intact plants and filled with 500 {mu}M MnSO{sub 4} for 5 days, and (iii) leaf disks floated for 3 days on 500 {mu}M MnSO{sub 4}. Density of brown speckles differed considerably among the six cultivars tested, and was not related to the Mn concentrations of the leaf tissues. There were close relationships between genotypic Mn-toxicity symptom expression and depression of dry matter production of the cultivars at high Mn supply in a long-term sand culture experiment. The floating leaf-disk method is particularly suited for screening large numbers of cowpea cultivars for Mn leaf-tissue tolerance because it requires only 3 days. The ranking of the cultivars for Mn tolerance was highly correlated to Mn tolerance of intact plants.

  7. A study on Maruca vitrata infestation of Yard-long beans (Vigna unguiculata subspecies sesquipedalis).

    Science.gov (United States)

    Jayasinghe, R C; Premachandra, W T S Dammini; Neilson, Roy

    2015-09-01

    Globally, Maruca vitrata (Geyer) is a serious yield constraint on food legumes including Yard-long bean (Vigna unguiculata subspecies sesquipedalis). However, there is a dearth of information on its damage potential, distribution and population dynamics in Yard-long beans. In the present study, the level of M. vitrata larval infestation on flowers and pods of Yard-long beans in Sri Lanka was determined with respect to three consecutive cropping seasons, Yala, Off and Maha. Results indicated that larval infestation and abundance varied with developmental stage of flowers and pods, cropping season and their combined interactive effects. Flowers of Yard-long beans were more prone to M. vitrata larval attack compared to pods. Abundance and level of infestation of M. vitrata varied with plant parts, having a ranking of flower buds (highest) > open flowers > mature pods > immature pods (lowest). Peak infestation was observed six and eight weeks after planting on flowers and pods, respectively. Among the three cropping seasons, M. vitrata infestation was found to be higher during Maha and Off seasons compared to Yala. The findings of this study contribute to the identified knowledge gap regarding the field biology of an acknowledged important pest, M. vitrata, in a previously understudied crop in Sri Lanka. PMID:27441212

  8. Sprouting characteristics and associated changes in nutritional composition of cowpea (Vigna unguiculata).

    Science.gov (United States)

    Devi, Chingakham Basanti; Kushwaha, Archana; Kumar, Anil

    2015-10-01

    Cowpea (Vigna unguiculata), is an important arid legume with a good source of energy, protein, vitamins, minerals and dietary fibre. Sprouting of legumes enhances the bioavailability and digestibility of nutrients and therefore plays an important role in human nutrition. Improved varieties of grain cowpea viz. Pant Lobia-1 (PL-1) and Pant Lobia-2 (PL-2) and Pant Lobia-3 (PL-3) were examined for sprouting characteristics and associated changes in nutritional quality. Soaking time, sprouting time and sprouting temperature combinations for desirable sprout length of ¼ to ½ inch for cowpea seed samples were standardized. All the observations were taken in triplicate except soaking time, where six observations were taken in a completely randomized design of three treatments. Results revealed that optimum soaking time of PL-1 and PL-2 seed was 3 h whereas PL-3 required 9 h. Sprouting period of 24 h at 25 °C was found to be desirable for obtaining good sprouts. Significant improvement in nutritional quality was observed after sprouting at 25 °C for 24 h; protein increased by 9-12 %, vitamin C increased by 4-38 times, phytic acid decreased by 4-16 times, trypsin inhibitor activity decreased by 28-55 % along with an increase of 8-20 % in in-vitro protein digestibility. PMID:26396436

  9. Toxic effects of Pb{sup 2+} on growth of cowpea (Vigna unguiculata)

    Energy Technology Data Exchange (ETDEWEB)

    Kopittke, Peter M. [School of Land, Crop and Food Sciences and CRC for Contamination Assessment and Remediation of the Environment, University of Queensland, St. Lucia, Queensland 4072 (Australia)], E-mail: p.kopittke@uq.edu.au; Asher, Colin J. [School of Land, Crop and Food Sciences, University of Queensland, St. Lucia, Queensland 4072 (Australia); Kopittke, Rosemary A. [Department of Primary Industries and Fisheries, 80 Meiers Road, Indooroopilly, Queensland 4068 (Australia); Menzies, Neal W. [School of Land, Crop and Food Sciences and CRC for Contamination Assessment and Remediation of the Environment, University of Queensland, St. Lucia, Queensland 4072 (Australia)

    2007-11-15

    A concentration as low as 1 {mu}M lead (Pb) is highly toxic to plants, but previous studies have typically related plant growth to the total amount of Pb added to a solution. In the present experiment, the relative fresh mass of cowpea (Vigna unguiculata) was reduced by 10% at a Pb{sup 2+} activity of 0.2 {mu}M for the shoots and at a Pb{sup 2+} activity of 0.06 {mu}M for the roots. The primary site of Pb{sup 2+} toxicity was the root, causing severe reductions in root growth, loss of apical dominance (shown by an increase in branching per unit root length), the formation of localized swellings behind the root tips (due to the initiation of lateral roots), and the bending of some root tips. In the root, Pb was found to accumulate primarily within the cell walls and intercellular spaces. - The Pb{sup 2+} ion reduced the growth of cowpea by 10% at a solution activity of 0.2 {mu}M for the shoots and 0.06 {mu}M for the roots.

  10. Effects of ozone on growth, net photosynthesis and yield of two African varieties of Vigna unguiculata.

    Science.gov (United States)

    Tetteh, Rashied; Yamaguchi, Masahiro; Wada, Yoshiharu; Funada, Ryo; Izuta, Takeshi

    2015-01-01

    To assess the effects of O(3)on growth, net photosynthesis and yield of two African varieties of cowpea(Vigna unguiculata L.), Blackeye and Asontem were exposed as potted plants to air that was either filtered to remove O(3) (FA), non-filtered air (NF), non-filtered with added O3 of approximately 50 nL L(-1) (ppb) from 11:00 to 16:00 (NF + O(3)) for 88 days in open-top chambers. The mean O(3) concentration (11:00-16:00) during the exposure period had a range from 16 ppb in the FA treatment to 118 ppb in the NF + O(3) treatment. Net photosynthetic rate and leaf area per plant were significantly reduced by exposure to O(3), reducing the growth of both varieties. Exposure to O(3) significantly reduced the 100-seed weight and number of seeds per pod. As a result, cowpea yield was significantly reduced by long-term exposure to O(3), with no difference in sensitivity between the varieties. PMID:25463718

  11. Prioritising in situ conservation of crop resources: a case study of African cowpea (Vigna unguiculata).

    Science.gov (United States)

    Moray, C; Game, E T; Maxted, N

    2014-01-01

    Conserving crop wild relatives (CWR) is critical for maintaining food security. However, CWR-focused conservation plans are lacking, and are often based on the entire genus, even though only a few taxa are useful for crop improvement. We used taxonomic and geographic prioritisation to identify the best locations for in situ conservation of the most important (priority) CWR, using African cowpea (Vigna unguiculata (L.) Walp.) as a case study. Cowpea is an important crop for subsistence farmers in sub-Saharan Africa, yet its CWR are under-collected, under-conserved and under-utilised in breeding. We identified the most efficient sites to focus in situ cowpea CWR conservation and assessed whether priority CWR would be adequately represented in a genus-based conservation plan. We also investigated whether priority cowpea CWR are likely to be found in existing conservation areas and in areas important for mammal conservation. The genus-based method captured most priority CWR, and the distributions of many priority CWR overlapped with established conservation reserves and targets. These results suggest that priority cowpea CWR can be conserved by building on conservation initiatives established for other species. PMID:24936740

  12. Effects of cowpea (Vigna unguiculata) root mucilage on microbial community response and capacity for phenanthrene remediation.

    Science.gov (United States)

    Sun, Ran; Belcher, Richard W; Liang, Jianqiang; Wang, Li; Thater, Brian; Crowley, David E; Wei, Gehong

    2015-07-01

    Biodegradation of polycyclic aromatic hydrocarbons (PAHs) is normally limited by their low solubility and poor bioavailability. Prior research suggests that biosurfactants are synthesized as intermediates during the production of mucilage at the root tip. To date the effects of mucilage on PAH degradation and microbial community response have not been directly examined. To address this question, our research compared 3 cowpea breeding lines (Vigna unguiculata) that differed in mucilage production for their effects on phenanthrene (PHE) degradation in soil. The High Performance Liquid Chromatography results indicated that the highest PHE degradation rate was achieved in soils planted with mucilage producing cowpea line C1, inoculated with Bradyrhizobium, leading to 91.6% PHE disappearance in 5 weeks. In root printing tests, strings treated with mucilage and bacteria produced larger clearing zones than those produced on mucilage treated strings with no bacteria or bacteria inoculated strings. Experiments with 14C-PHE and purified mucilage in soil slurry confirmed that the root mucilage significantly enhanced PHE mineralization (82.7%), which is 12% more than the control treatment without mucilage. The profiles of the PHE degraders generated by Denaturing gradient gel electrophoresis suggested that cowpea C1, producing a high amount of root mucilage, selectively enriched the PHE degrading bacteria population in rhizosphere. These findings indicate that root mucilage may play a significant role in enhancing PHE degradation and suggests that differences in mucilage production may be an important criterion for selection of the best plant species for use in phytoremediation of PAH contaminated soils. PMID:26141877

  13. Removal of nickel(II) from aqueous solution by Vigna unguiculata (cowpea) pods biomass.

    Science.gov (United States)

    Guyo, Upenyu; Sibanda, Kudakwashe; Sebata, Edith; Chigondo, Fidelis; Moyo, Mambo

    2016-01-01

    The potential to remove nickel(II) ions from aqueous solution using a biosorbent prepared from Vigna unguiculata pods (VUPs) was investigated in batch experiments. The batch mode experiments were conducted utilising the independent variables of pH (2 to 8), contact time (5 to 120 min), dosage concentration (0.2 to 1.6 g), nickel(II) concentrations (10 to 80 mg L(-1)) and temperature (20 to 50°C). The biosorption data fitted best to the Freundlich biosorption model with a correlation coefficient (R(2)) of 0.993 and lowest chi-squared value of 31.89. The maximum sorption capacity of the VUP for nickel(II) was 27.70 mg g(-1). Kinetics studies revealed that the biosorption process followed the pseudo-second-order model as it had the lowest sum of square error value (0.808) and correlation coefficient close to unity (R(2) = 0.998). The calculated thermodynamic parameters showed that the biosorption process was feasible, spontaneous and endothermic. Consequently, the study demonstrated that VUP biomass could be used as a biosorbent for the removal of nickel(II) from aqueous solution. PMID:27191550

  14. Highly distinct chromosomal structures in cowpea (Vigna unguiculata), as revealed by molecular cytogenetic analysis.

    Science.gov (United States)

    Iwata-Otsubo, Aiko; Lin, Jer-Young; Gill, Navdeep; Jackson, Scott A

    2016-05-01

    Cowpea (Vigna unguiculata (L.) Walp) is an important legume, particularly in developing countries. However, little is known about its genome or chromosome structure. We used molecular cytogenetics to characterize the structure of pachytene chromosomes to advance our knowledge of chromosome and genome organization of cowpea. Our data showed that cowpea has highly distinct chromosomal structures that are cytologically visible as brightly DAPI-stained heterochromatic regions. Analysis of the repetitive fraction of the cowpea genome present at centromeric and pericentromeric regions confirmed that two retrotransposons are major components of pericentromeric regions and that a 455-bp tandem repeat is found at seven out of 11 centromere pairs in cowpea. These repeats likely evolved after the divergence of cowpea from common bean and form chromosomal structure unique to cowpea. The integration of cowpea genetic and physical chromosome maps reveals potential regions of suppressed recombination due to condensed heterochromatin and a lack of pairing in a few chromosomal termini. This study provides fundamental knowledge on cowpea chromosome structure and molecular cytogenetics tools for further chromosome studies. PMID:26758200

  15. Toxic effects of low concentrations of Cu on nodulation of cowpea (Vigna unguiculata)

    Energy Technology Data Exchange (ETDEWEB)

    Kopittke, Peter M. [School of Land and Food Sciences, University of Queensland, St. Lucia, Qld 4072 (Australia)]. E-mail: p.kopittke@uq.edu.au; Dart, Peter J. [School of Land and Food Sciences, University of Queensland, St. Lucia, Qld 4072 (Australia); Menzies, Neal W. [School of Land and Food Sciences, University of Queensland, St. Lucia, Qld 4072 (Australia)

    2007-01-15

    Although Cu is phytotoxic at Cu{sup 2+} activities as low as 1-2 {mu}M, the effect of Cu{sup 2+} on the nodulation of legumes has received little attention. The effect of Cu{sup 2+} on nodulation of cowpea (Vigna unguiculata (L.) Walp. cv. Caloona) was examined in a dilute solution culture system utilising a cation exchange resin to buffer solution Cu{sup 2+}. The nodulation process was more sensitive to increasing Cu{sup 2+} activities than both shoot and root growth; whilst a Cu{sup 2+} activity of 1.0 {mu}M corresponded to a 10% reduction in the relative yield of the shoots and roots, a Cu{sup 2+} activity of 0.2 {mu}M corresponded to a 10% reduction in nodulation. This reduction in nodulation with increasing Cu{sup 2+} activity was associated with an inhibition of root hair formation in treatments containing {>=}0.77 {mu}M Cu{sup 2+}, rather than to a reduction in the size of the Rhizobium population. - The nodulation process was more sensitive to increasing Cu{sup 2+} activities than either shoot or root growth.

  16. Effect of gamma irradiation and cooking on cowpea bean grains ( Vigna unguiculata L. Walp)

    Science.gov (United States)

    Lima, Keila dos Santos Cople; Souza, Luciana Boher e.; Godoy, Ronoel Luiz de Oliveira; França, Tanos Celmar Costa; Lima, Antônio Luís dos Santos

    2011-09-01

    Leguminous plants are important sources of proteins, vitamins, carbohydrates, fibers and minerals. However, some of their non-nutritive elements can present undesirable side effects like flatulence provoked by the anaerobic fermentation of oligosaccharides, such as raffinose and stachyose, in the gut. A way to avoid this inconvenience, without any change in the nutritional value and post-harvesting losses, is an irradiation process. Here, we evaluated the effects of gamma irradiation on the amino acids, thiamine and oligosaccharide contents and on the fungi and their toxin percentages in cowpea bean ( Vigna unguiculata L. Walp) samples. For irradiation doses of 0.0, 0.5, 1.0, 2.5, 5.0 and 10.0 kGy the results showed no significant differences in content for the uncooked samples. However, the combination of irradiation and cooking processes reduced the non-nutritive factors responsible for flatulence. Irradiation also significantly reduced the presence of Aspergillus, Penicilium, Rhizopus and Fusarium fungi and was shown to be efficient in grain conservation for a storage time of 6 months.

  17. Effects of irradiation on physical and sensory characteristics of cowpea seed cultivars ( Vigna unguiculata L. Walp)

    Science.gov (United States)

    Ocloo, F. C. K.; Darfour, B.; Ofosu, D. O.; Wilson, D. D.

    2012-01-01

    Cowpeas ( Vigna unguiculata L. Walp) are leguminous seeds widely produced and consumed in most developing countries of sub-Saharan Africa where they are a good source of affordable proteins, minerals and vitamins to the mainly carbohydrate-based diet of sub-Saharan Africa. At storage cowpea may be attacked by insects that cause severe damage to the seeds. The objective of this study was to investigate the effects of gamma irradiation on some physical and sensory characteristics of cowpea seed cultivars. Four cowpea cultivars were irradiated with gamma radiation at dose levels of 0.25, 0.50, 0.75, 1.0 and 1.5 kGy. Moisture content, thousand grain weight and bulk densities were determined as well as the amount of water absorbed during soaking and some sensory characteristics were equally determined. All the physical parameters studied were not significantly ( p>0.05) affected by the radiation. There was no significant ( p>0.05) effect of the radiation on the sensory attributes like flavour, taste, texture, softness and colour of the cowpea seeds. Similarly, the radiation did not affect significantly ( p>0.05) the acceptability of the treated cowpea cultivars.

  18. Simultaneous selection for cowpea (Vigna unguiculata L.) genotypes with adaptability and yield stability using mixed models.

    Science.gov (United States)

    Torres, F E; Teodoro, P E; Rodrigues, E V; Santos, A; Corrêa, A M; Ceccon, G

    2016-01-01

    The aim of this study was to select erect cowpea (Vigna unguiculata L.) genotypes simultaneously for high adaptability, stability, and yield grain in Mato Grosso do Sul, Brazil using mixed models. We conducted six trials of different cowpea genotypes in 2005 and 2006 in Aquidauana, Chapadão do Sul, Dourados, and Primavera do Leste. The experimental design was randomized complete blocks with four replications and 20 genotypes. Genetic parameters were estimated by restricted maximum likelihood/best linear unbiased prediction, and selection was based on the harmonic mean of the relative performance of genetic values method using three strategies: selection based on the predicted breeding value, having considered the performance mean of the genotypes in all environments (no interaction effect); the performance in each environment (with an interaction effect); and the simultaneous selection for grain yield, stability, and adaptability. The MNC99542F-5 and MNC99-537F-4 genotypes could be grown in various environments, as they exhibited high grain yield, adaptability, and stability. The average heritability of the genotypes was moderate to high and the selective accuracy was 82%, indicating an excellent potential for selection. PMID:27173301

  19. A study on Maruca vitrata infestation of Yard-long beans (Vigna unguiculata subspecies sesquipedalis).

    Science.gov (United States)

    Jayasinghe, R C; Premachandra, W T S Dammini; Neilson, Roy

    2015-09-01

    Globally, Maruca vitrata (Geyer) is a serious yield constraint on food legumes including Yard-long bean (Vigna unguiculata subspecies sesquipedalis). However, there is a dearth of information on its damage potential, distribution and population dynamics in Yard-long beans. In the present study, the level of M. vitrata larval infestation on flowers and pods of Yard-long beans in Sri Lanka was determined with respect to three consecutive cropping seasons, Yala, Off and Maha. Results indicated that larval infestation and abundance varied with developmental stage of flowers and pods, cropping season and their combined interactive effects. Flowers of Yard-long beans were more prone to M. vitrata larval attack compared to pods. Abundance and level of infestation of M. vitrata varied with plant parts, having a ranking of flower buds (highest) > open flowers > mature pods > immature pods (lowest). Peak infestation was observed six and eight weeks after planting on flowers and pods, respectively. Among the three cropping seasons, M. vitrata infestation was found to be higher during Maha and Off seasons compared to Yala. The findings of this study contribute to the identified knowledge gap regarding the field biology of an acknowledged important pest, M. vitrata, in a previously understudied crop in Sri Lanka.

  20. Seed Size Influence On Germination And Seedling Development Of Cowpea (Vigna unguiculata (L walp

    Directory of Open Access Journals (Sweden)

    Olayemi Ibukun Ehoniyotan

    2013-09-01

    Full Text Available The effect of seed size on the germination and seedling development was carried out on four local varieties of cowpea (Vigna unguiculata (L walp, oloyin,drum, ifebrown and local white. Each of the varieties were sorted into big and small seeds, 100 seeds each of both big and small seeds of the four Varieties were weighed and the average weights were calculated and recorded. Six seeds of each sort were planted in a plastic pot filled with sandy-loamy soil in three replicates. Results of the various parameters studied revealed that germination efficiency was similar in both big and small seeds of drum, oloyin and ifebrown but not in small seeds of local white, weights of 100 seeds from big seeds were much heavier than those of small seeds. Number of leaves, leaf length, leaf breadth, leaf area, stem height and dry weight, were consistently higher in cowpea plants from big seeds than the small seeds of all the varieties. Number of seeds per pod was higher in cowpea from big seeds in all the varieties except in drum where number of seeds was higher in plants from small seeds. In general, seedlings emerging from big seeds of all the cowpea varieties performed better in all the parameters studied than those emerging from smaller seeds.

  1. BIOLOGÍA DEL ÁFIDO NEGRO (Aphis craccivora: APHIDIDAE) SOBRE FRÍJOL CAUPI (Vigna unguiculata, FABACEAE)

    OpenAIRE

    Nataly DE LA PAVA S; Paula Andrea SEPÚLVEDA-CANO

    2015-01-01

    Este trabajo describe los parámetros biológicos y poblacionales del pulgón negro, Aphis craccivora Koch, sobre fríjol caupí (Vigna unguiculata (L. Walp.)), bajo condiciones de laboratorio. Los insectos se criaron sobre plántulas individualizadas a una temperatura promedio de 28 °C, humedad relativa de 70 % y fotoperiodo de 12 horas. Los parámetros medidos fueron periodo ninfal, fecundidad, supervivencia, periodo pre-reproductivo y aspectos relativos a la tasa de crecimiento del áfido. La tasa...

  2. Effects of the legume Vigna unguiculata crop on carbon and nitrogen cycles

    Science.gov (United States)

    Sánchez-Navarro, Virginia; Zornoza, Raúl; Fernández, Juan; Faz Cano, Ángel

    2015-04-01

    In this study, we investigated the effects of a legume crop (Vigna unguiculata) on soil properties related to the carbon (C) and nitrogen (N) cycles, taking into account different management practices (conventional and organic) and two genotypes. The study was randomly designed in blocks with four replications, in plots of 10 m2. The crop cycle spanned from 29 May 2014 to 13 August 2014. We collected soil samples (0-30 cm) from each plot at the beginning and at the end of the cycle to measure soil total N, organic C, recalcitrant C, organic C labile fractions, microbial biomass C (MBC) and the enzyme activities β-glucosidase and β-glucosaminidase. We collected plant samples (seeds, pods, roots and stem/leaves) at two different maturity stages (fresh and dry pods) to assess the influence of management practices and genotype in the accumulation of N, as indicative of the content of proteins in the crop. In the final plant sampling, we also determined crop production. The results showed that no significant differences were observed between management practices and genotypes in any of the soil properties measured. However, total N, recalcitrant C, most labile C fraction, MBC and β-glucosidase increased at the final sampling compared to initial values. We observed that genotype had a significant effect on the concentration of the second fraction of labile C under organic management. N content in the different plant tissues was significantly higher in the intermediate sampling than in the final harvest, without significant differences between management practices and genotypes. We observed a significant positive correlation between N content in roots, seeds and pods. N content was always higher in seeds, indicating the high quantity of proteins in this crop. C content was significantly lower in stem/leaves than in the rest of tissues, without significant differences among them. No effect of management practice, maturity stage or genotype was observed with regard to C

  3. Evaluation of aminoacids in irradiated beans (Vigna unguiculata (L.) Walp) by high performance liquid chromatography (HPLC)

    Energy Technology Data Exchange (ETDEWEB)

    Lima, Keila S. Cople; Souza, Luciana B.; Coelho, Maysa J.; Lima, Antonio L. Santos; Hernandes, Nilber K. [Instituto Militar de Engenharia (IME), Rio de Janeiro, RJ (Brazil). Secao de Engenharia Nuclear]. E-mail: keila@ime.eb.br; Godoy, Ronoel L.O. [EMBRAPA Agroindustria de Alimentos, Rio de Janeiro, RJ (Brazil)]. E-mail: ronoel@ctaa.embrapa.br

    2007-07-01

    Fradinho-bean (Vigna unguiculata (L.) Walp) is originated from Africa and is known in Brazil as 'caupi', 'corda' or 'macassar'. It is grown in the interior of Northeast Brazil (semi-arid region) and can be found in parts of the North, being one of the most important components of people's diet in those regions. The Northeast area produces around 429,375 ton of fradinho-bean per year. Leguminous plants are very important sources of proteins, vitamins, carbohydrates and minerals. This kind of bean is an excellent source of proteins (around 23- 25% of its nutritional content), being superior to regular beans (Phaseolus vulgaris). The irradiation process is an alternative to avoid post-harvesting losses, without changing the nutritional value of food. This study has the objective to evaluate the effect of different gamma irradiation doses (0.0; 0.5; 1.0; 2.5; 5.0 and 10.0 kGy) on aminoacid content of fradinho-bean by high performance liquid chromatography (HPLC) and the accompanying of the grains during storage time of 6 months. After irradiation, the bean grains went through a milling process in order to make flour for posterior extraction. A liquid chromatographer Waters, model Alliance 2695, with fluorescent detector Waters 2475, having a mobile phase with gradient elution of sodium acetate. acetonitrile and Milli-Q water, was employed. The flux used was 1 mL/min and the injection volume of 10 {mu}L. The column (C 18 150.0 x 3.9 mm) was kept at 36 deg C. The results show that gamma irradiation is a promise process for fradinho bean during conservation storage time of 6 months, until the dose of 10.0 kGy. Even the most radio-sensitive aminoacids like aromatics and basic lateral chains were preserved. (author)

  4. Estudo do feijão verde (Vigna unguiculata (L Walp minimamente processado

    Directory of Open Access Journals (Sweden)

    FURTUNATO Andréa A

    2000-01-01

    Full Text Available Visando definir tecnologias alternativas de processamento do feijão verde (Vigna unguiculata (L Walp, estudou-se o processo de congelamento do mesmo. Este consistiu na recepção da matéria prima, seleção dos grãos de coloração e tamanho uniformes, seguida de lavagem em água corrente e branqueamento por imersão em água a 90ºC. Os grãos foram acondicionados em sacos plásticos e congelados em freezer doméstico a -18ºC, sendo armazenados a essa temperatura. Foi realizado o estudo de vida de prateleira desse produto por 180 dias, através de análises físico-químicas, microbiológicas e sensoriais, preservando-se em boas condições para o consumo. Foi realizado também o teste de aceitação do produto comparando-se com o feijão verde fresco. Embora o nível de aceitação não tenha sido muito alto (71,1% quando comparado com o feijão fresco (96,6%, este resultado poderia ser diferente em regiões que não têm o hábito de consumir esta leguminosa no estádio de maturação verde fresco.

  5. Diallelic analysis to obtain cowpea (Vigna unguiculata L. Walp.) populations tolerant to water deficit.

    Science.gov (United States)

    Rodrigues, E V; Damasceno-Silva, K J; Rocha, M M; Bastos, E A

    2016-01-01

    The purpose of this study was to identify parents and obtain segregating populations of cowpea (Vigna unguiculata L. Walp.) with the potential for tolerance to water deficit. A full diallel was performed with six cowpea genotypes, and two experiments were conducted in Teresina, PI, Brazil in 2011 to evaluate 30 F2 populations and their parents, one under water deficit and the other under full irrigation. A triple-lattice experimental design was used, with six 2-m-long rows in each plot. Sixteen plants were sampled per plot. The data were subjected to analysis of variance, and general and specific combining ability estimates were obtained based on the means. Additive effects were more important than non-additive effects, and maternal inheritance had occurred. The genotypes BRS Xiquexique, Pingo de Ouro-1-2, and MNC99-510F-16-1 were the most promising for use in selection programs aimed at water deficit tolerance. The hybrid combinations Pingo de Ouro-1-2 x BRS Xiquexique, BRS Xiquexique x Santo Inácio, CNCx 698-128G x MNC99-510F-16-1, Santo Inácio x CNCx 698-128G, MNC99-510F-16-1 x BRS Paraguaçu, MNC99- 510F-16-1 x Pingo de Ouro-1-2, and MNC99-510F-16-1 x BRS Xiquexique have the potential to increase grain production and tolerate water deficit. PMID:27323025

  6. Variabilidade e correlações entre caracteres agronômicos em caupi (Vigna unguiculata

    Directory of Open Access Journals (Sweden)

    Lopes Ângela Celis de Almeida

    2001-01-01

    Full Text Available O caupi (Vigna unguiculata (L. Walp é um alimento básico das populações do Nordeste brasileiro, devendo merecer atenção com vistas a melhoria da qualidade de grãos, resistência a doenças e pragas e aumento de produtividade. Este trabalho teve por objetivo estudar a variabilidade e o potencial genético de 28 linhagens, escolhidas após uma seleção para cor, tamanho de grãos e resistência a viroses. A produtividade apresentou coeficiente de variação genético de 23,90%, e o valor agronômico, de 3,56%. O número de vagens por pedúnculo apresentou a menor estimativa do coeficiente de determinação genético (4,51%, e o peso de 100 grãos, a maior (81,74%. O coeficiente de determinação genético da produtividade foi de 34,15%. As maiores estimativas de ganho genético foram as do peso de 100 grãos (21,73% e da produtividade (19,77%. As correlações genotípicas foram superiores às fenotípicas e às de ambiente, destacando-se as correlações entre número de ramos secundários e produtividade (68,13%, e valor agronômico e produtividade (100%. Estes resultados mostram amplas possibilidades de seleção entre as linhagens com relação à maioria dos caracteres estudados.

  7. Effects of irradiation on physical and sensory characteristics of cowpea seed cultivars (Vigna unguiculata L. Walp)

    Energy Technology Data Exchange (ETDEWEB)

    Ocloo, F.C.K., E-mail: fidelis_ocloo@yahoo.com [Radiation Technology Centre, Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission. P.O. Box LG 80, Legon (Ghana); Darfour, B.; Ofosu, D.O. [Radiation Technology Centre, Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission. P.O. Box LG 80, Legon (Ghana); Wilson, D.D. [Department of Zoology, University of Ghana, Legon (Ghana)

    2012-01-15

    Cowpeas (Vigna unguiculata L. Walp) are leguminous seeds widely produced and consumed in most developing countries of sub-Saharan Africa where they are a good source of affordable proteins, minerals and vitamins to the mainly carbohydrate-based diet of sub-Saharan Africa. At storage cowpea may be attacked by insects that cause severe damage to the seeds. The objective of this study was to investigate the effects of gamma irradiation on some physical and sensory characteristics of cowpea seed cultivars. Four cowpea cultivars were irradiated with gamma radiation at dose levels of 0.25, 0.50, 0.75, 1.0 and 1.5 kGy. Moisture content, thousand grain weight and bulk densities were determined as well as the amount of water absorbed during soaking and some sensory characteristics were equally determined. All the physical parameters studied were not significantly (p>0.05) affected by the radiation. There was no significant (p>0.05) effect of the radiation on the sensory attributes like flavour, taste, texture, softness and colour of the cowpea seeds. Similarly, the radiation did not affect significantly (p>0.05) the acceptability of the treated cowpea cultivars. - Highlights: > We investigated the effects of gamma irradiation on some physical and sensory characteristics of cowpea seed cultivars. > Four cowpea cultivars were irradiated at dose levels of 0.25, 0.50, 0.75, 1.0 and 1.5 kGy. > Physical parameters were not significantly (p>0.05) affected by the radiation. > Sensory attributes considered were not significantly influenced by the radiation doses used.

  8. Generation Means Analysis of Seed Sucrose Content in Cowpea (Vigna unguiculata L. Walp.

    Directory of Open Access Journals (Sweden)

    J.B. Noubissie Tchiagam

    2011-11-01

    Full Text Available The sucrose concentration of seeds is an important component of the taste in cowpea (Vigna unguiculata L. Walp.. The objective of this research was to estimate the heritability, to investigate the mode of genetic control and to evaluate the potential for genetic improvement of sucrose content in cowpea using generation’s means analysis. F1 and F2 generations as well as backcross populations (BCP1 and BCP2 were produced in three hybrid combinations by crosses of four selected cultivars. The sucrose level was measured via a High Performance Liquid Chromatography (HPLC system. The range of variation in sucrose content among tested varieties was from 1.88 mg/g (Lori to 5.32 mg/g (C93W-24-125. Broad-sense heritability (h2 was of a moderate 58.50% to high 68.12% value and no transgressive segregation was observed. Narrow-sense heritability (39.47 to 47.83% and heterosis (-20.00 to - 5.94% values and gene effects analysis suggested that the sucrose content is controlled by additive and non-additive genes. At 10% level of selection, an increase of 19.60 to 33.64% was predicted after one cycle. Epistasis was significant in most of crosses and at least five genes affected the sucrose accumulation in cowpea seeds. These results suggested that breeding for increased sucrose content in the seeds of cowpea can be quite successful through recurrent selection in later generations.

  9. Population structure analysis and association mapping of seed antioxidant content in USDA cowpea (Vigna unguiculata L. Walp.) core collection using SNPs

    Science.gov (United States)

    Cowpea (Vigna unguiculata (L) Walp.) is an important legume and the antioxidants in cowpea seeds have been recognized as health-promoting compounds for human. The objectives of this study were to analyze the population structure of cowpea collections using single nucleotide polymorphism (SNP) and to...

  10. Effect of Nitrogen Nutritional Stress on Some Growth Parameters of Zea mays L. and Vigna unguiculata (L. Walp.

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    Akinbode Foluso OLOGUNDUDU

    2013-02-01

    Full Text Available This study investigated the responses of maize (Zea mays L. and cowpea (Vigna unguiculata L. Walp. seedlings growth parameters to nitrogen nutritional stress. This was with a view to determining whether nitrogen nutritional stress would retard or enhance maize and cowpea growth, partly, wholly or not at all through its effect on biomass accumulation and some morphological parameters. Germination of seeds was done using treated sand in sixty plastic pots. A group of the seedlings was nutrient stressed by administering 200 ml of complete nutrient solution minus nitrogen (-N while the other groups were fed with five times (X5N and ten times (X10N the optimal concentration of nitrogen and the last regime was fed with full nutrient solution (FN. The effects of optimal concentration and nitrogen stress on the growth rates (as measured by their fresh and dry weight were studied. The result of the growth analysis showed that there was increase in shoot height with supraoptimal concentrations of nitrogen treatments (X10N and X5N while there was a decrease in shoot height with minus nitrogen (-N regimes. The observed higher biomass (dry matter yield under the FN regimes in both Zea mays and Vigna unguiculata were attributed to optimal nutrient assimilation rate.

  11. Isoenzymes of superoxide dismutase in nodules of Phaseolus vulgaris L. , Pisum sativum L. , and Vigna unguiculata (L. ) Walp

    Energy Technology Data Exchange (ETDEWEB)

    Becana, M.; Paris, F.J.; Sandalio, L.M.; Del Rio, L.A. (IRNA, Salamanca (Spain) Unidad de Bioquimica Vegetal, Granada (Spain))

    1989-08-01

    The activity and isozymic composition of superoxide dismutase were determined in nodules of Phaseolus vulgaris L., Pisum sativum L., and Vigna unguiculata (L.) Walp. A Mn-SOD was present in Rhizobium and two in Bradyrhizobium and bacteroids. Nodule mitochondria from all three legume species had a single Mn-SOD with similar relative mobility, whereas the cytosol contained several CuZn-SODs: two in Phaseolus and Pisum, and four in Vigna. In the cytoplasm of V. unguiculata nodules, a Fe-containing SOD was also present, with an electrophoretic mobility between those of CuZn- and Mn-SODs, and an estimated molecular weight of 57,000. Total SOD activity of the soluble fraction of host cells, expressed on a nodule fresh weight basis, exceeded markedly that of bacteroids. Likewise, specific SOD activities of free-living bacteria were superior or equal to those of their symbiotic forms. Soluble extracts of bacteria and bacteroids did not show peroxidase activity, but the nodule cell cytoplasm contained diverse peroxidase isozymes which were readily distinguishable from leghemoglobin components by electrophoresis. Data indicated that peroxidases and leghemoglobins did not significantly interfere with SOD localization on gels. Treatment with chloroform-ethanol scarcely affected the isozymic pattern of SODs and peroxidases, and had limited success in the removal of leghemoglobin.

  12. MoO3 Nanoparticles: Synthesis, Characterization and Its Hindering Effect on Germination of Vigna Unguiculata Seeds.

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    Abhimanyu Kanneganti

    2014-07-01

    Full Text Available Molybdenum trioxide nanoparticles have been synthesized by sol-gel method. The X-ray diffraction analysis was done to confirm that the obtained product was MoO3. The scanning electron microscopy was done to study the shape, size distribution and surface morphology of nanoparticles; they had a hexagonal shape with smooth surface and uniform size distribution. The functional groups were studied using Fourier transform infrared spectroscopy. The effect of MoO3 nanoparticles on seed germination of vigna unguiculata was studied for 6 days from the day of sowing, by comparing the time taken for seeds to germinate and length of shoot with respect to time of the seeds sowed in heavy black soil whose nutrient composition was known with seeds sowed in the same heavy black soil but which was made rich with MoO3 nanoparticles. It was observed that the MoO3 nanoparticles hampered the germination of vigna unguiculata seeds and this restraint continued in the shoot growth also.

  13. Effect of Nitrogen Nutritional Stress on some Mineral Nutrients and Photosynthetic Apparatus of Zea mays L. and Vigna unguiculata L.

    Directory of Open Access Journals (Sweden)

    Akinbode Foluso OLOGUNDUDU

    2013-08-01

    Full Text Available The study investigated the responses of maize (Zea mays L. and cowpea (Vigna unguiculata L. Walp. seedlings metabolic activities and photosynthetic apparatus to nitrogen nutritional stress. Germination of seeds was done using treated sand in sixty plastic pots and the seedlings were divided into four nutrient regimes. A group of the seedlings was nutrient stressed by administering 200 ml of complete nutrient solution minus nitrogen (-N while the other groups were fed with five times (X5N and ten times (X10N the optimal concentration of nitrogen and the last regime was fed with full nutrient solution (FN. The photosynthetic parameters studied included chlorophylls ‘a’ and ‘b’ respectively; carotenes and xanthophyll while the mineral elements investigated include potassium, calcium and magnesium. The result of the growth analysis showed that nitrogen deficiency promotes an increase in the content of abscisic acid (ABA, causing stomatal closure and a reduction in photosynthesis. This explains the higher rate of leaf abscission in -N plants. A comparison of calcium ion and magnesium ion concentrations in both optimal and stressed conditions reveals that the two ions show antagonism in uptake. There is a correlation between nitrogen and magnesium accumulation as magnesium ion plays a vital role in chlorophyll biosynthesis, protein synthesis and photosynthesis. The pattern of accumulation of photosynthetic apparatus in both maize and cowpea follow a similar pattern. Chlorophyll a dictated the growth pattern of other photosynthetic apparatus in both Zea mays and Vigna unguiculata.

  14. Soil compaction and gamma radiation ({sup 60}Co) in the development of the cowpea beans [Vigna unguiculata, (L) Walp]; Compactacao do solo e radiacao gama ({sup 60}Co) no desenvolvimento do feijao caupi [Vigna unguiculata, (L) Walp

    Energy Technology Data Exchange (ETDEWEB)

    Viana, Eliane Ferreira; Colaco, Waldeciro [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear. Radioagronomia]. E-mails: aroucha@ufpe.br; wcolaco@ufpe.br

    2005-08-15

    The objective is to investigate the effect of compaction and increased doses of gamma radiation on the development of cowpea [Vigna unguiculata, (L) Walp] var. IPA-206 cultivated in a Neossolo fluvic soil, artificially compacted. Significant differences were observed in plant height, with the increase doses in soil density (1.30 Mg.m{sup -3} - compacted soil and 1.70 Mg.m{sup -3} - non-compacted soil), and in response to increased doses of irradiation (y-rays) [ [0, 100, 200 and 300 Gy). The diameter of the stem was significantly reduced in response to the increase in soil density, however the same did not occur in relation to the doses of irradiation (y-rays) holding capacity of the soil was reduced in response to the increase in soil density. (author)

  15. Effect of storage on the amino acid composition and biological quality of irradiated macacar beans Vigna unguiculata (L.) Walp

    International Nuclear Information System (INIS)

    The effects of two doses of gamma radiation (100 and 1,000 krad) upon the stability over a 6-month storage period of the amino acid composition and protein efficiency ratio (PER) of the macacar bean Vigna unguiculata (L.) Walp were investigated. No important differences were noted when the aminograms of irradiated and nonirradiated beans, either raw or cooked were compared. Nevertheless, the losses of lysine, arginine, and histidine due to cooking were greater in the irradiated beans. The PER of nonirradiated was higher than that of irradiated beans before and after the 6 months of storage, and was always lowest in the beans subjected to the higher dose of radiation. Qualitatively, an association was observed between the nutritional value (PER) and small decreases in the content of certain amino acids which resulted mainly from increased thermal lability of the irradiated bean protein

  16. Antioxidant Activity of the Extracts of Some Cowpea (Vigna unguiculata (L Walp. Cultivars Commonly Consumed in Pakistan

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    Vincenzo De Feo

    2013-02-01

    Full Text Available The present investigation has been carried out to determine the antioxidant activity of the methanolic extracts obtained from four cultivars of cowpea (Vigna unguiculata (L Walp. seeds. Phenolic compounds present in the extracts showed the antioxidant and antiradical properties when investigated using a linoleic acid peroxidation model, FRAP, ORAC and TRAP assays, as well as DPPH, hydroxyl, nitric oxide and superoxide radical scavenging activity. The HPLC analysis of the cowpea extracts showed the presence of neochlorogenic acid, chlorogenic acid and caffeic acids. The results indicated that methanolic extract of the cowpea resembled in the aforementioned activities those from other leguminous seeds and pulses. Phenolic constituents contained in cowpea may have a future role as ingredients in the development of functional foods.

  17. EFFECT OF DIETARY LEVELS OF COWPEA (Vigna unguiculata SEEDS ON BROILER PERFORMANCE AND SOME SERUM BIOCHEMICAL FACTORS

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    N. GUMAA BALAIEL

    2014-01-01

    Full Text Available Effect of inclusion of different levels of untreated cowpea (Vigna unguiculata seeds (0, 5, 10 and 15% in broiler diet on performance and some serum biochemical factors was studied. The research was conducted on basis of a completely Randomized Design (CRD. Feed intake, body weight gain, feed conversion ratio, protein intake and protein efficiency ratio were significantly (P<0.05 reduced with the inclusion of 15% untreated cowpea seeds. Plasma cholesterol, glucose, albumin, total protein, Ca and K contents were significantly (P<0.05 decreased with increasing level of cowpea seeds in diets. Uric acid concentration observed to be higher in birds fed 15% cowpea seed. It is concluded that good performance of broiler chicken is satisfactory maintained with 5 to 10% inclusion of cowpea seeds in balanced diet for broiler.

  18. Effect of PGR producing bacterial strains isolated from vermisources on germination and growth of Vigna unguiculata (L. Walp.

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    Anandharaj Marimuthu

    2014-12-01

    Full Text Available Nineteen bacterial strains were isolated from vermisources andscreened for Indole-3-acetic acid (IAA production among themonly nine strains produce IAA and they were identified asStreptococcus spp., Micrococcus spp., Klebsiella spp., Bacillus spp., Enterobacter spp., Escherichia spp., Alcaligenes spp., Erwinia spp., and Pseudomonas spp. Among all other strains Bacillus sp. showed the higher IAA production hence selected for further molecular analysis and confirmed as Bacillus cereus. The B. cereus was grown in nutrient broth supplemented with different concentrations (1, 2, 3, 4 and 5mg/ml of tryptophan for seven days at pH 7 and at 37ºC. Crude IAA was used for in vitro phytostimulatory studies using Vigna unguiculata (L. Walp. The plant growth parameters were analyzed at different day intervals (5, 10 and 15 days. Supplementation of 5 ml crude IAA (2mg/ml of tryptophan dynamically enhances the plant growth parameters after 15 days.

  19. Feeding value of hays of tropical forage legumes in pigs: Vigna unguiculata, Psophocarpus scandens, Pueraria phaseoloides and Stylosanthes guianensis.

    Science.gov (United States)

    Kambashi, Bienvenu; Boudry, Christelle; Picron, Pascale; Kiatoko, Honoré; Bindelle, Jérôme

    2014-12-01

    The effects of four tropical forage legume hays (Vigna unguiculata, Psophocarpus scandens, Pueraria phaseoloides and Stylosanthes guianensis) on voluntary feed intake (VFI) and their nutritive value were studied in growing pigs using a corn-soybean meal-based diet containing varying proportions of forage legume hays (0, 10, 20 and 40 % or 0, 12.5 and 25 % for VFI and nutritive value determination, respectively). There was no difference in VFI between species (P > 0.20), but a linear response to forage inclusion level (P unguiculata, where the response was quadratic (P = 0.01). All four forage species linearly decreased the total tract apparent digestibility (TTAD) from 0.76 to 0.61, 0.80 to 0.68, 0.54 to 0.40 and 0.58 to 0.31 except for S. guianensis (0.44) for DM, N, NDF and N retention, respectively. Differences in digestibility (P < 0.05) between species were also observed. Due to their negative influence on the overall digestibility, the contribution of hays should not exceed 12.5 %, except for S. guianensis, in which N retention remained quite high (0.44) at the highest inclusion level (25 %). P. phaseoloides hay should be avoided in pigs as it combines the lowest VFI with the lowest nutrient digestibility. PMID:25069970

  20. In vivo studies on the occurrence of stored mRNA in embryonic axes of Vigna unguiculata seeds

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Yoshiharu; Minamikawa, Takao (Tokyo Metropolitan Univ. (Japan). Faculty of Science)

    1983-12-01

    ..cap alpha..-Amanitin and cordycepin at various concentrations were tested for their inhibitory effect on the fresh weight increase of Vigna unguiculata embryonic axes after the onset of imbibition and on the incorporation rate of /sup 3/H-labeled leucine into protein in axes of the 36 - 38 h stage. ..cap alpha..-Amanitin at 0.5 - 5 ..mu..g/ml clearly exerted an inhibitory effect on both the fresh weight increase and the protein synthesis. This drug at 1 ..mu..g/ml, however, showed no significant effect on the protein synthesis at an early stage of imbibition (4 h), whereas cycloheximide was a very potent inhibitor. By experiments in which 'dry' axes were allowed to imbibe /sup 3/H-labeled adenosine solution for 4 and 12 h in the presence of ..cap alpha..-amanitin, it was found that poly A+RNA was newly synthesized to some extent in axes as early as 4 h after the onset of imbibition and that the drug effectively inhibited the poly A+RNA synthesis. The results may indicate the occurrence of stored mRNA in embryonic axes of V. unguiculata seeds.

  1. Effect of biologically treated petroleum sludge on seed germination and seedling growth of Vigna unguiculata (L. Walp. (Fabaceae

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    Jeyabalan Sangeetha

    2014-06-01

    Full Text Available The present investigation was carried out to study the response of different concentrations of treated petroleum sludge on seed germination, root and shoot length and tolerance of Vigna unguiculata (L. Walp. The biologically treated petroleum sludge with bacterial consortium showed 54.8% reduction in total petroleum hydrocarbons. Treated sludge was utilized with agricultural soil in known concentration for the assessment of growth of V. unguiculata. A remarkable absence of seed germination was observed at higher sludge concentration. The different concentrations of treated petroleum sludge showed severe decline on the length, weight and vigour index of the tested seedlings with increasing sludge concentrations. The results showed that the difference in rate of seed germination was significant among various concentrations. Under environmental stress condition, germination is the most critical phase of life cycle in crop plants. In this present study, the high oil content found to alter the osmotic relation between seed and water and thus reduce the amount of water absorbed. It was concluded that the concentration of nutrients and oil present in the treated sludge were toxic to the plant.

  2. Simple and rapid methods for purification and characterization of active coagulants from the seeds of Vigna unguiculata and Parkinsonia aculeata.

    Science.gov (United States)

    Marobhe, N J; Dalhammar, G; Gunaratna, K R

    2007-06-01

    The coagulating properties of aqueous crude extracts and purified proteins of Vigna unguiculata and Parkinsonia aculeata seeds, which are traditional water coagulants in rural areas of Tanzania, were studied. The coagulation activity assays were done using one millilitre (ml) of kaolin water samples. Coagulating proteins were purified in two-step ion exchange chromatography. The properties of coagulant protein were compared with Moringa oleifera. Coagulating components eluted by 0.6 M NaCl in both coagulants are cationic proteins that have the molecular mass of about 6 kDa, which is very similar to that of M. oleifera. The proteins of V. unguiculata and P. aculeata eluted by 0.3 M NaCl also harbour coagulation activity but proteins eluted with 0.6 M NaCl have higher activity. The dosage for coagulation using purified proteins of both coagulants is about 5 to 10 times lower than that of crude seed extracts. The optimum floc settling time of water treated by crude seed extracts and purified proteins ranged between two and two and half hours. Coagulating proteins of both coagulants eluted by 0.6 M NaCl are thermoresistant and retained coagulation activity of 87% to 92% after boiling for two hours at 80 degrees C and one hour at 95 degrees C. Thermotolerant proteins of V. unguiculata eluted by 0.6 M NaCl and P. aculeata have wider pH range of 5.5 to 8.5 for coagulation activity than those of M. oleifera proteins. The present investigation reveals the possibility of using purified natural coagulants for water treatment to produce safe drinking water.

  3. CGKB: an annotation knowledge base for cowpea (Vigna unguiculata L. methylation filtered genomic genespace sequences

    Directory of Open Access Journals (Sweden)

    Spraggins Thomas A

    2007-04-01

    Full Text Available Abstract Background Cowpea [Vigna unguiculata (L. Walp.] is one of the most important food and forage legumes in the semi-arid tropics because of its ability to tolerate drought and grow on poor soils. It is cultivated mostly by poor farmers in developing countries, with 80% of production taking place in the dry savannah of tropical West and Central Africa. Cowpea is largely an underexploited crop with relatively little genomic information available for use in applied plant breeding. The goal of the Cowpea Genomics Initiative (CGI, funded by the Kirkhouse Trust, a UK-based charitable organization, is to leverage modern molecular genetic tools for gene discovery and cowpea improvement. One aspect of the initiative is the sequencing of the gene-rich region of the cowpea genome (termed the genespace recovered using methylation filtration technology and providing annotation and analysis of the sequence data. Description CGKB, Cowpea Genespace/Genomics Knowledge Base, is an annotation knowledge base developed under the CGI. The database is based on information derived from 298,848 cowpea genespace sequences (GSS isolated by methylation filtering of genomic DNA. The CGKB consists of three knowledge bases: GSS annotation and comparative genomics knowledge base, GSS enzyme and metabolic pathway knowledge base, and GSS simple sequence repeats (SSRs knowledge base for molecular marker discovery. A homology-based approach was applied for annotations of the GSS, mainly using BLASTX against four public FASTA formatted protein databases (NCBI GenBank Proteins, UniProtKB-Swiss-Prot, UniprotKB-PIR (Protein Information Resource, and UniProtKB-TrEMBL. Comparative genome analysis was done by BLASTX searches of the cowpea GSS against four plant proteomes from Arabidopsis thaliana, Oryza sativa, Medicago truncatula, and Populus trichocarpa. The possible exons and introns on each cowpea GSS were predicted using the HMM-based Genscan gene predication program and the

  4. Growth and yield response of cowpea (Vigna unguiculata L. Walp.) to soils from different fallow physiognomies in the rainforest zone of Nigeria

    OpenAIRE

    Samson Olajide OKE; Eyitayo, Damilola Lanre

    2010-01-01

    A study was conducted to evaluate the response of cowpea (Vigna unguiculata) seedlings to soil collected from four fallows of different physiognomy. Seedlings of cowpea weregrown from seeds on soil samples collected from the four different fallow statuses(Panicum maximum-dominated fallow soil, Chromolaena odorata-dominated fallow soil,Tithonia spp.-dominated fallow soil and bush fallow soil that contains many herbaceousplant species) in plastic containers each having fifteen replicates. Among...

  5. Intra- and interchromosomal rearrangements between cowpea [Vigna unguiculata (L.) Walp.] and common bean (Phaseolus vulgaris L.) revealed by BAC-FISH.

    Science.gov (United States)

    Vasconcelos, Emanuelle Varão; de Andrade Fonsêca, Artur Fellipe; Pedrosa-Harand, Andrea; de Andrade Bortoleti, Kyria Cilene; Benko-Iseppon, Ana Maria; da Costa, Antônio Félix; Brasileiro-Vidal, Ana Christina

    2015-06-01

    Cowpea (Vigna unguiculata) is an annual legume grown in tropical and subtropical regions, which is economically relevant due to high protein content in dried beans, green pods, and leaves. In this work, a comparative cytogenetic study between V. unguiculata and Phaseolus vulgaris (common bean) was conducted using BAC-FISH. Sequences previously mapped in P. vulgaris chromosomes (Pv) were used as probes in V. unguiculata chromosomes (Vu), contributing to the analysis of macrosynteny between both legumes. Thirty-seven clones from P. vulgaris 'BAT93' BAC library, corresponding to its 11 linkage groups, were hybridized in situ. Several chromosomal rearrangements were identified, such as translocations (between BACs from Pv1 and Pv8; Pv2 and Pv3; as well as Pv2 and Pv11), duplications (BAC from Pv3), as well as paracentric and pericentric inversions (BACs from Pv3, and Pv4, respectively). Two BACs (from Pv2 and Pv7), which hybridized at terminal regions in almost all P. vulgaris chromosomes, showed single-copy signal in Vu. Additionally, 17 BACs showed no signal in V. unguiculata chromosomes. The present results demonstrate the feasibility of using BAC libraries in comparative chromosomal mapping and karyotype evolution studies between Phaseolus and Vigna species, and revealed several macrosynteny and collinearity breaks among both legumes. PMID:25634499

  6. 襄阳市豇豆生产现状及发展思路%Production Status and Development Thoughts of Vigna unguiculata in Xiangyang

    Institute of Scientific and Technical Information of China (English)

    杨伟; 王四清

    2011-01-01

    豇豆[Vigna unguiculata(L.)Walp.]是襄阳市重要的蔬菜作物之一,目前已成为当地春夏秋季主供蔬菜种类之一,并且是春秋淡季主要的度淡种类,市场需求量很大,其产业发展呈逐步扩大趋势.文章结合襄阳市发展豇豆生产的现状和有利条件,分析了豇豆产业发展中存在的主要问题,提出了豇豆产业在品种资源、新技术应用、科研开发、推进产业化进程与壮大龙头加工企业上的发展思路,从而更好地带动豇豆产业快速健康发展.%Vigna unguiculata (L.) Walp. Was one of the most important vegetable crops in Xiangyang city and had become one of the main supplying vegetables in spring and autumn. The industry development of V. Unguiculata was expanding year by year. The main problems existuing in V. Unguiculata industry development combined with the present situation and favorable conditions in Xiangyang were analyzed. Ideas in varieties resources, new technology, research and development, promoting industrialization and strengthening the processing enterprises of the V. Unguiculata industrial development were proposed in order to promote the V. Unguiculata industry rapid and healthy development.

  7. OUTWARD CURRENT CONDUCTING ION CHANNELS IN TONOPLASTS OF VIGNA-UNGUICULATA

    NARCIS (Netherlands)

    MAATHUIS, FJM; PRINS, HBA

    1991-01-01

    Patch clamp studies were done on tonoplasts of V unguiculata. Apart from the usually present inward rectifying K+ conducting channels (IRC), vacuoles showed outward rectifying channels (ORC), although less frequently. The ORC are activated at physiological tonoplast potentials, in contrast to IRC, a

  8. Nitric oxide increases tolerance responses to moderate water deficit in leaves of Phaseolus vulgaris and Vigna unguiculata bean species.

    Science.gov (United States)

    Zimmer-Prados, Lucas Martins; Moreira, Ana Sílvia Franco Pinheiro; Magalhaes, Jose Ronaldo; França, Marcel Giovanni Costa

    2014-07-01

    Drought stress is one of the most intensively studied and widespread constraints, and nitric oxide (NO) is a key signaling molecule involved in the mediation of abiotic stresses in plants. We demonstrated that a sprayed solution of NO from donor sodium nitroprusside increased drought stress tolerance responses in both sensitive (Phaseolus vulgaris) and tolerant (Vigna unguiculata) beans. In intact plants subjected to halting irrigation, NO increased the leaf relative water content and stomatal conductance in both species. After cutting leaf discs and washing them, NO induced increased electrolyte leakage, which was more evident in the tolerant species. These leaf discs were then subjected to different water deficits, simulating moderate and severe drought stress conditions through polyethylene glycol solutions. NO supplied at moderate drought stress revealed a reduced membrane injury index in sensitive species. In hydrated discs and at this level of water deficit, NO increased the electron transport rate in both species, and a reduction of these rates was observed at severe stress levels. Taken together, it can be shown that NO has an effective role in ameliorating drought stress effects, activating tolerance responses at moderate water deficit levels and in both bean species which present differential drought tolerance. PMID:25049456

  9. The effects of Vigna unguiculata on cardiac oxidative stress and aorta estrogen receptor-β expression of ovariectomized rats

    Directory of Open Access Journals (Sweden)

    Etik Khusniyati

    2014-12-01

    Conclusions: V. unguiculata is an alternative therapy in decreasing cardiac oxidative stress in ovariectomized rats. Besides, high dose of V. unguiculata also able to increase aorta estrogen receptor-β expression in ovariectomized rats.

  10. Soil Management and Efficiency of Rhizobia Strains of Cowpea Vigna unguiculata (L. Walp. in the Tropics Manejo de Suelo y Eficiencia de Cepas de Rizobio de Frijol Vigna unguiculata (L. Walp. en los Trópicos

    Directory of Open Access Journals (Sweden)

    José Geraldo Donizetti dos Santos

    2011-12-01

    Full Text Available In the humid tropics, the largest obstacle to the implementation of sustainable farming systems is the reduced efficiency of nutrient use by crops. This study assesses the effectiveness of five selected rhizobia strains in symbiosis with cowpea (Vigna unguiculata (L. Walp., with the objective of replacing N fertilization in the predominant agricultural system used by smallholder farmers. The study was carried out in three adjacent areas with distinct agricultural uses: conventional tillage, itinerant agriculture, and a no-till system. The experimental design was in randomized complete blocks with four replicates and seven treatments: five rhizobia strains (BR3262, BR3267, BR3299, INPA3-11B, and UFLA 3-84 and two controls without inoculation (one without mineral N and another fertilized with 74 kg N ha-1. We measured the dry mass of 100 grains, nodules and shoots, as well as cowpea yields and calculated relative and absolute efficiency indices for dry biomass production of cowpea shoots. Agricultural uses affected the number and dry mass of the nodules and, consequently, the mass of the dry plant matter and bean yield. In terms of yield, there was a major difference between the conventional and the itinerant systems. Yield was around four times as high in the itinerant system (1009.9 kg ha-1 compared to 243.7 kg ha-1. Under conditions of cohesion-prone soils, the system of conventional tillage reduces the possibility of cultivation of a second harvest in the year even with inoculation or N fertilization.En los trópicos húmedos, el mayor obstáculo para la aplicación de sistemas de agricultura sostenible es la reducción de la eficiencia del uso de nutrientes por los cultivos. Este estudio evalúa la eficacia de cinco cepas de rizobios seleccionados en simbiosis con frijol (Vigna unguiculata (L. Walp., con el objetivo de sustituir la fertilización nitrogenada en el sistema de uso de suelo predominante utilizado por los pequeños agricultores

  11. Nodulação e produtividade de Vigna unguiculata (L. Walp. por cepas de rizóbio em Bom Jesus, PI Yield and nodulation of Vigna unguiculata (L. Walp. inoculated with rhizobia strains in Bom Jesus, PI

    Directory of Open Access Journals (Sweden)

    Elaine Martins Costa

    2011-03-01

    Full Text Available Objetivou-se avaliar a resposta de Vigna unguiculata (L. Walp. cv. "BR 17 Gurguéia" à inoculação com duas cepas isoladas de solos de mineração de bauxita em reabilitação: UFLA 3-164 e UFLA 3-155 e três cepas INPA 03 11B (BR 3301; UFLA 03 84 (BR 3302 e BR 3267 (SEMIA 6462, autorizadas pelo MAPA como inoculantes para a cultura do feijão-caupi. O experimento foi conduzido em campo na Universidade Federal do Piauí, Campus Professora Cinobelina Elvas, Bom Jesus, PI. Utilizou-se o delineamento experimental em blocos casualizados com sete tratamentos e com seis repetições, sendo cinco cepas citadas e dois controles não inoculados, um com N-mineral (70 kg ha-1 de N e outra sem N mineral. Foram avaliados a nodulação (número e massa seca de nódulos, o crescimento (massa seca da parte aérea, o rendimento de grãos e o teor e acúmulo de nitrogênio na parte aérea e nos grãos, além da eficiência relativa. A inoculação das sementes com as cepas de bactérias diazotróficas simbióticas resultou em rendimentos de grãos equivalente à testemunha adubada com nitrogênio mineral. A cepa em fase de teste, UFLA 3-155 apresentou rendimento de grãos igual à cepa recomendada INPA 03 11B (BR 3301, podendo também ser testada em outras regiões brasileiras. Entre as cepas aprovadas pelo MAPA a INPA 03 11B (BR 3301 apresentou a maior produção de grãos.It evaluates the effect of inoculation with two rhizobia strains isolated from soils under rehabilitation after bauxite mining: UFLA 3-164 and UFLA 3-155, compared to inoculation with strains INPA 03 11B (BR 3301; UFLA 03 84 (BR 3302 and BR 3267 (SEMIA 6462, officially authorized as inoculant to cowpea by MAPA, in Vigna unguiculata (L. Walp cv. "BR 17 Gurgueia". The experiment was carried out at the 'Universidade Federal do Piauí, Campus Professora Cinobelina Elvas, Bom Jesus, PI,' in a randomized block design, white seven treatments and six replications. Treatments were the five strains and

  12. Vigna mungo, V. radiata and V. unguiculata plants sampled in different agronomical-ecological-climatic regions of India are nodulated by Bradyrhizobium yuanmingense.

    Science.gov (United States)

    Appunu, Chinnaswamy; N'Zoue, Angèle; Moulin, Lionel; Depret, Géraldine; Laguerre, Gisèle

    2009-10-01

    Vigna mungo, Vigna radiata and Vigna unguiculata are important legume crops cultivated in India, but little is known about the genetic resources in native rhizobia that nodulate these species. To identify these bacteria, a core collection of 76 slow-growing isolates was built from root nodules of V. mungo, V. radiata and V. unguiculata plants grown at different sites within three agro-ecological-climatic regions of India. The genetic diversity of the bacterial collection was assessed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified DNA fragments of the 16S-23S rDNA intergenic spacer (IGS) region, and the symbiotic genes nifH and nodC. One rDNA IGS type grouped 91% of isolates, but more diversity was found at the symbiotic loci (17 symbiotic genotypes). Overall, no host plant specificity was shown, the three host plant species sharing common bradyrhizobial genotypes that represented 62% of the collection. Similarly, the predominant genotypes were found at most sampling sites and in all agro-ecological-climatic regions. Phylogenies inferred from IGS sequencing and multi-locus sequence analysis of the dnaK, glnII and recA genes indicated that all isolates but one were clustered with the Bradyrhizobium yuanmingense species. The nifH phylogeny also grouped the different nif haplotypes within a cluster including B. yuanmingense, except for one infrequent nif haplotype which formed a new lineage within the Bradyrhizobium genus. These results may reflect a long history of co-evolution between B. yuanmingense and Vigna spp. in India, while intra-species polymorphism detected in the symbiotic loci may be linked with the long history of diversification of B. yuanmingense coinciding with that of its host legumes.

  13. High performance liquid chromatography (HPLC) applied to determination of oligosaccharides in irradiated beans (Vigna unguiculata (L.) Walp)

    Energy Technology Data Exchange (ETDEWEB)

    Lima, Keila S. Cople; Souza, Luciana B.; Coelho, Maysa J.; Lima, Antonio L. Santos; Vital, Helio C. [Instituto Militar de Engenharia IME, Rio de Janeiro, RJ (Brazil). Secao de Engenharia Nuclear]. E-mail: keila@ime.eb.br; maysa@ime.eb.br; santoslima@ime.eb.br; Godoy, Ronoel L.O. [EMBRAPA Agroindustria de Alimentos, Rio de Janeiro, RJ (Brazil)]. E-mail: ronoel@ctaa.embrapa.br

    2007-07-01

    Beans are important components of Brazilian diet, especially for less affordable people. They have in their composition proteins, vitamins, carbohydrates, minerals and fibers. Despite of their high nutritional value, nonnutritive elements from natural origin are present in leguminous plants, such as raffinose and stachyose. The oligosaccharides are flatulence agents due to bacterial anaerobic fermentation at the intestines. The aim of this study is to evaluate the effect of combined boiling and gamma irradiation treatments on fradinho-beans (Vigna unguiculata (L.) Walp) with respect to oligosaccharide contents. The irradiation process was performed at the Nuclear Defense Section of Brazilian Army, using a cavity type research irradiator, which has a Cs-137 radiation source and maximum dose rate of 1.8 kGy/h. The oligosaccharides were evaluated by HPLC technique, at 'EMBRAPA Agroindustria de Alimentos', using a SHIMADZU liquid chromatography system, with a refraction index detector Waters 2421. The mobile phase was acetonitrile 70% in water for raffinose and stachyose and 80% for saccharose. The flux and the running time were 1 mL/min and 18 minutes for raffinose and stachyose, and 1.3 mL/min and 20 minutes for saccharose. The injection volume was 20 {mu}L and the column used (Waters 250 x 4,6 mm; 4 mm) was kept at room temperature. The evaluation of unboiled irradiated beans showed that there was no significant difference between the different irradiation doses and control sample, keeping the oligosaccharide contents. However, the combination of the boiling and the irradiation processes turned out to reduce of non-nutritive factors that may cause flatulence, meaning a real benefit to the consumers. (author)

  14. Evaluation of Different Tillage Practices for Monocultural Cowpea (Vigna unguiculata (L. Walp Production in Ibadan, South Western Nigeria

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    Ndaeyo, NU.

    1997-01-01

    Full Text Available A two-season (rainy and dry study was conducted in 1993 at the Teaching and Research Farm, University of Ibadan, Nigeria to assess the most productive tillage practice for monocultural cowpea (Vigna unguiculata (L. Walp production. Completely randomised block design with four replications was used and tillage treatments were : No till-Slash and Burn (NSB, No till-Herbicide applied (NH, Conventional-ploughed and harrowed (CT, and Minimum-ploughed only (MT. Results revealed that tillage practices had no significant effect on percentage emergence, leaf and branch number in both seasons. In the first season, plant height was significantly (P <0.05 influenced by tillage treatments at 2 and 4 weeks after sowing (WAS with NH showing superiority over the other treatments. In the second season, plant height was significantly (P <0.05 higher in NSB and MT treatments at 6 and 8 WAS, respectively. Pod and grain yield (t ha-1 were not affected by tillage treatments in the first season but in the second season, NSB (1.84 t ha-1 and MT (1.53 t ha-1 showed significant superiority over other treatments. The highest cost of production was observed under NSB while CT produced the highest economie returns. Some soil properties were also influenced with NSB treatment having a higher soil bulk density at sowing and 6 WAS than the other treatments, while NH recorded a higher soil moisture content at 6 WAS than the other treatments. The study also suggests that with optimum precipitation, CT appears a better land preparation option for cowpea production.

  15. Nodulation of cowpeas and survival of cowpeas Rhizobia in acid, aluminum-rich soils. [Vigna unguiculata; Rhizobium

    Energy Technology Data Exchange (ETDEWEB)

    Hartel, P.G.; Whelan, A.M.; Alexander, M.

    1983-01-01

    A study was undertaken to determine whether the reduced nodulation of cowpeas (Vigna unguiculata (L.) Walp) grown in certain acid, Alrich soils resulted from the poor survival of the potentially infective rhizobia. Two strains of Rhizobium capable of nodulating cowpeas were used. The lowest pH for growth in defined liquid medium was 4.2 for one strain and 3.9 for the other. Only the latter was Al tolerant and could grow in a defined liquid medium containing 50 ..mu..M KAl(SO/sub 4/)/sub 2/. The survival of the bacteria and their ability to nodulate cowpeas in three soils were measured after the soils were amended with Ca or Al salts to give pH values ranging from 5.7 to 4.1 and extractable-Al concentrations from < 0.1 to 3.7 cmol(p/sup +/)/kg of soil. Only small differences in survival in 7 or 8 weeks were noted between the two strains. Plants inoculated with the Al-sensitive strain bore significantly fewer nodules in the more acid, Al-rich soils than in the same soils with higher pH values and less extractable Al. No significant reduction in nodule number was evident for plants inoculated with the Al-tolerant strain and grown in the more acid, Al-rich soils compared to cowpeas grown in the same soils with higher pH values and less extractable Al. It is suggested that the Al content of soil is not a major factor in the survival of cowpea rhizobia but that it does have a significant effect on nodulation. 24 references, 3 figures, 2 tables.

  16. Thermal stability of ascorbic acid and ascorbic acid oxidase in african cowpea leaves ( Vigna unguiculata ) of different maturities.

    Science.gov (United States)

    Wawire, Michael; Oey, Indrawati; Mathooko, Francis; Njoroge, Charles; Shitanda, Douglas; Hendrickx, Marc

    2011-03-01

    Cowpea, an African leafy vegetable ( Vigna unguiculata ), contains a high level of vitamin C. The leaves harvested at 4-9 weeks are highly prone to vitamin C losses during handling and processing. Therefore, the purpose of this research was to study the effect of thermal treatment on the stability of ascorbic acid oxidase (AAO), total vitamin C content (l-ascorbic acid, l-AA), and dehydroascorbic acid (DHAA) and l-AA/DHAA ratio in cowpea leaves harvested at different maturities (4, 6, and 8 weeks old). The results showed that AAO activity, total vitamin C content, and l-AA/DHAA ratio in cowpea leaves increased with increasing maturity (up to 8 weeks). Eight-week-old leaves were the best source of total vitamin C and showed a high ratio of l-AA/DHAA (4:1). Thermal inactivation of AAO followed first-order reaction kinetics. Heating at temperatures above 90 °C for short times resulted in a complete AAO inactivation, resulting in a protective effect of l-AA toward enzyme-catalyzed oxidation. Total vitamin C in young leaves (harvested at 4 and 6 weeks) was predominantly in the form of DHAA, and therefore temperature treatment at 30-90 °C for 10 min decreased the total vitamin C content, whereas total vitamin C in 8-week-old cowpea leaves was more than 80% in the form of l-AA, so that a high retention of the total vitamin C can be obtained even after heating and/or reheating (30-90 °C for 10 min) before consumption. The results indicated that the stability of total vitamin C in situ was strongly dependent on the plant maturity stage and the processing conditions applied. PMID:21309563

  17. EFFECT OF PHOSPHORUS AND PLANT DENSITY ON THE YIELD COMPONENTS OF COW-PEA (Vigna unguiculata L. Walp): MORFOLOGICAL CHARACTERISTICS EFEITO DO FÓSFORO E DA DENSIDADE DE POPULAÇÃO SOBRE OS COMPONENTES DO RENDIMENTO DO FEIJÃO-DE-CORDA (Vigna unguiculata L. Walp): CARACTERÍSTICAS MORFOLÓGICAS

    OpenAIRE

    Benedito Carlos Lemos de Carvalho; Magda Beatriz de Almeida Matteucci

    2007-01-01

    A field experiment was carried out on a distrophic Red Yellow Latosol Text, in the State of Bahia, Brazil, to evaluate the effects of population densities and levels of phosphorous dressing on some morphological characteristics of cow-pea (Vigna unguiculata L Walp), Cv “Tv x 1836-013J”, with a determinate growing habit. Population densities of 40, 80, 160 and 320 thousands of plants per hectare and phosphorus level...

  18. Atuação de rizóbios com rizobactéria promotora de crescimento em plantas na cultura do caupi (Vigna unguiculata [L.] Walp.) = Rhizobias performance with rhizobacteria growth promoter in plants in the cowpea crop (Vigna unguiculata [L.] Walp.)

    OpenAIRE

    Valéria Nogueira da Silva; Luiz Eduardo Souza Fernandes da Silva; Márcia do Vale Barreto Figueiredo

    2006-01-01

    O objetivo deste estudo foi verificar a potencialidade da colonização conjunta em feijão caupi (Vigna unguiculata [L.] Walp.) por Paenibacillus e Bradyrhizobium em diferentes métodos de inoculação que proporcionam crescimento visando a uma agricultura sustentável. Os trabalhos foram realizados na Empresa Pernambucana de PesquisaAgropecuária (IPA), com Argissolo Amarelo (Itapirema, Estado de Pernambuico), dois modos de inoculação (semente e solo) em cultivar de caupi (IPA-205). Foi determinado...

  19. Vicilins (7S storage globulins of cowpea (Vigna unguiculata seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae larvae

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    Sales M.P.

    2001-01-01

    Full Text Available The presence of chitin in midgut structures of Callosobruchus maculatus larvae was shown by chemical and immunocytochemical methods. Detection by Western blotting of cowpea (Vigna unguiculata seed vicilins (7S storage proteins bound to these structures suggested that C. maculatus-susceptible vicilins presented less staining when compared to C. maculatus-resistant vicilins. Storage proteins present in the microvilli in the larval midgut of the bruchid were recognized by immunolabeling of vicilins in the appropriate sections with immunogold conjugates. These labeling sites coincided with the sites labeled by an anti-chitin antibody. These results, taken together with those previously published showing that the lower rates of hydrolysis of variant vicilins from C. maculatus-resistant seeds by the insect's midgut proteinases and those showing that vicilins bind to chitin matrices, may explain the detrimental effects of variant vicilins on the development of C. maculatus larvae.

  20. Compatibility of Intercropping Stem Borer Resistant Sorghum Sorghum bicolor Moench Genotypes with Cowpea Vigna unguiculata (L Walp and its Effect on Flower Thrips

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    Ampong-Nyarko, K.

    1994-01-01

    Full Text Available The compatibility of sorghum Sorghum bicolor Moench genotypes with varying levels of resistance to stem borers for intercropping was studied in field experiments for two cropping seasons at the ICIPE in Kenya. Sorghum genotypes IS-18520, IS-1044, IS-2269, ICS 3, ICS 4, LRB6, 2Kx17and Gaddam El Hamam were grown both as monocrops and as intercrops with cowpea. Intercropping reduced the Chilo partellus population density but there was no significant genotype x intercropping interaction. Intercropping significantly reduced the number of flower thrips Megalurothrips sjostedti in cowpea Vigna unguiculata (L Walp. The stem borer resistance level of sorghum genotypes tested was not affected by intercropping. There were, however, differences in agronomic productivity. Grain yield of intercropped sorghum was positively correlated with the number of tillers per harvestable head. Sorghum genotypes with high tillering capacity, of intermediate plant height and intermediate leaf area were considered compatible for intercropping with cowpea.

  1. Effet comparé des poudres de Nicotiana tabacum L, Cymbopogon citratus (D.C. Stapf et de l'huile de Ricinus communis L sur la conservation des graines de Vigna unguiculata (L Walp

    Directory of Open Access Journals (Sweden)

    Gakuru, S.

    1995-01-01

    Full Text Available Compared Effect of Nicotiana tabacum L, Cymbopogon citratus (D.C. Stapf Powders and Castor Oil Ricinus communis L. on Conservation of Cowpea Vigna Unguiculata (L. Walp Grains. The effect of powder of tobacco Nicotiana tabacum L. and citronella grass Cymbopogon citratus (D.C. Stapf and castor oil Ricinus communis L. on conservation of cowpea Vigna unguiculata (L. Walp. grains was investigated in Kisangani, Zaire. After 5 months of conservation, infestation rates by bean weevil Acanthoscelides obtectus Say were 72.5 %, 74.5 %, 49.5 % and 5 % respectively for the check, the samples treated by 1 % of citronella grass and tobacco powder and 1 % of castor oil. The powder dose of 7.5 % did not give more interesting results.

  2. Efectividad de cepas rizobianas nativas de sabana en Vigna unguiculata (L. Walp. cv. C4A-3

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    Juliana Mayz

    2010-12-01

    Full Text Available Título en inglés: Effectiveness of savannah native rhizobial strains in Vigna unguiculata (L. Walp. cv. C4A-3 Resumen Se estima que la población mundial se incrementará y demandará mayor cantidad de alimentos y uso de fertilizantes nitrogenados. En Venezuela, el frijol es altamente consumido y se cultiva en las sabanas orientales, cuyas características edáficas pueden afectar negativamente la población rizobiana. Estos planteamientos refuerzan la importancia de la evaluación de la flora rizobiana nativa, y enfatizan la necesidad de aumentar la explotación de la fijación biológica de nitrógeno. En este contexto, se evaluaron 6 cepas rizobianas en el cultivar C4A-3, aisladas, de frijol cv. Tejero Criollo y previamente catalogadas como efectivas (JV91, JV94 y JV101 e inefectivas (JV99, JV103, y JV104 en el cultivar TC9-6. El experimento se llevó a cabo en umbráculo por 45 días, donde además se incluyeron dos tratamientos control no inoculados. La suspensión de las cepas individualmente cultivadas se usó para inoculación. De acuerdo con la tipología de la nodulación (número de nódulos, peso total y por nódulo, tamaño y color, los valores de los parámetros de crecimiento (peso seco, altura y número de hojas del vástago y los estimados de la concentración de nitrógeno y nitrógeno total, las cepas JV91, JV99 y JV101, fueron las más efectivas en la fijación de nitrógeno. El nitrógeno total y la concentración de nitrógeno tuvieron una correlación significativa con peso seco, altura y número de hojas del vástago. Los resultados muestran la existencia de cepas efectivas en los suelos de sabana para este cultivar, y enfatizan la importancia de evaluar las cepas indígenas, antes de proceder a la inoculación con foráneas. Palabras clave: Rhizobium; frijol; fijación de nitrógeno; Venezuela. Abstract It is estimated that world-wide population will increase and demand higher amount of food and use of nitrogen

  3. Effect of preparation practices and the cowpea cultivar Vigna unguiculata L.Walp on the quality and content of myo-inositol phosphate in akara (fried bean paste

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    Walison Fabio Rogério

    2014-06-01

    Full Text Available Akara is one of Brazil's national treasures prepared from cowpea (Vigna unguiculata L.Walp, grated onions and salt and deep-fried in crude palm oil. The results of this study on akara preparation methods showed that, in general, cowpeas were soaked for up 3 hours at room temperature, and the seed coats were then removed. The akara makers preferred the olho de pombo cultivar, because of its cream hue, or the macassar cultivar because it produces a crispier paste. The seeds purchased from street markets had lower ranges of InsP6, InsP5, and InsP4 (1.03-7.62 ∝mol.g- 1; 0.14-1.31 ∝mol.g- 1; and 0.0-0.10 ∝mol.g- 1, respectively than both the paste and akara (6.72-19.24 ∝mol.g- 1; 1.29-4.57 ∝mol.g- 1; 0.0-0.76 ∝mol.g- 1; 3.31-13.71 ∝mol.g- 1; 0.0-4.48 ∝mol.g- 1; and 0.0-1.32 ∝mol.g- 1. These results suggest that other beans or cowpea varieties have been used in the preparation of akara and that the phytate levels do not affect its nutritional quality.

  4. Polyphenolic extracts from cowpea (Vigna unguiculata) protect colonic myofibroblasts (CCD18Co cells) from lipopolysaccharide (LPS)-induced inflammation--modulation of microRNA 126.

    Science.gov (United States)

    Ojwang, Leonnard O; Banerjee, Nivedita; Noratto, Giuliana D; Angel-Morales, Gabriela; Hachibamba, Twambo; Awika, Joseph M; Mertens-Talcott, Susanne U

    2015-01-01

    Cowpea (Vigna unguiculata) is a drought tolerant crop with several agronomic advantages over other legumes. This study evaluated varieties from four major cowpea phenotypes (black, red, light brown and white) containing different phenolic profiles for their anti-inflammatory property on non-malignant colonic myofibroblasts (CCD18Co) cells challenged with an endotoxin (lipopolysaccharide, LPS). Intracellular reactive oxygen species (ROS) assay on the LPS-stimulated cells revealed antioxidative potential of black and red cowpea varieties. Real-time qRT-PCR analysis in LPS-stimulated cells revealed down-regulation of proinflammatory cytokines (IL-8, TNF-α, VCAM-1), transcription factor NF-κB and modulation of microRNA-126 (specific post-transcriptional regulator of VCAM-1) by cowpea polyphenolics. The ability of cowpea polyphenols to modulate miR-126 signaling and its target gene VCAM-1 were studied in LPS-stimulated endothelial cells transfected with a specific inhibitor of miR-126, and treated with 10 mg GAE/L black cowpea extract where the extract in part reversed the effect of the miR-126 inhibitor. This suggests that cowpea may exert their anti-inflammatory activities at least in part through induction of miR-126 that then down-regulate VCAM-1 mRNA and protein expressions. Overall, Cowpea therefore is promising as an anti-inflammatory dietary component. PMID:25300227

  5. Pathways of nitrogen assimilation in cowpea nodules studied using /sup 15/N/sub 2/ and allopurinol. [Vigna unguiculata L. Walp. cv Vita

    Energy Technology Data Exchange (ETDEWEB)

    Atkins, C.A.; Storer, P.J.; Pate, J.S.

    1988-01-01

    In the presence of 0.5 millimolar allopurinol (4-hydroxypyrazolo (3,4-d)pyrimidine), an inhibitor of NAD:xanthine oxidoreductase (EC 1.2.3.2), intact attached nodules of cowpea (vigna unguiculata L. Walp. cv Vita 3) formed (/sup 15/N)xanthine from /sup 15/N/sub 2/ at rates equivalent to those of ureide synthesis, confirming the direct assimilation of fixed nitrogen into purines. Xanthine accumulated in nodules and was exported in increasing amounts in xylem of allopurinol-treated plants. Other intermediates of purine oxidation, de novo purine synthesis, and ammonia assimilation did not increase and, over the time course of experiments (4 hours), allopurinol had no effect on nitrogenase (EC 1.87.99.2) activity. Negligible /sup 15/N -labeling of asparagine from /sup 15/N/sub 2/ was observed, suggesting that the significant pool (up to 14 micromoles per gram of nodule fresh weight) of this amide in cowpea nodules was not formed directly from fixation but may have accumulated as a consequence of phloem delivery.

  6. Cowpea (Vigna unguiculata L. Walp), a renewed multipurpose crop for a more sustainable agri-food system: nutritional advantages and constraints.

    Science.gov (United States)

    Gonçalves, Alexandre; Goufo, Piebiep; Barros, Ana; Domínguez-Perles, Raúl; Trindade, Henrique; Rosa, Eduardo A S; Ferreira, Luis; Rodrigues, Miguel

    2016-07-01

    The growing awareness of the relevance of food composition for human health has increased the interest of the inclusion of high proportions of fruits and vegetables in diets. To reach the objective of more balanced diets, an increased consumption of legumes, which constitutes a sustainable source of essential nutrients, particularly low-cost protein, is of special relevance. However, the consumption of legumes also entails some constraints that need to be addressed to avoid a deleterious impact on consumers' wellbeing and health. The value of legumes as a source of nutrients depends on a plethora of factors, including genetic characteristics, agro-climatic conditions, and postharvest management that modulate the dietary effect of edible seeds and vegetative material. Thus, more comprehensive information regarding composition, especially their nutritional and anti-nutritional compounds, digestibility, and alternative processing procedures is essential. These were the challenges to write this review, which focusses on the nutritional and anti-nutritional composition of Vigna unguiculata L. Walp, an emerging crop all over the world intended to provide a rational support for the development of valuable foods and feeds of increased commercial value. © 2016 Society of Chemical Industry. PMID:26804459

  7. Assessing the mutagenic effects of gamma irradiation on Cajanus cajan (L. Huth and Vigna unguiculata (L. Walp landraces using morphological markers

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    Ogbuagu Udensi Ugorji

    2012-12-01

    Full Text Available Giving the high adaptability, heritability, genetic variability and nutritive values reported in the indigenous landraces of legumes, combined efforts should be geared towards their improvement.The present study aimed at evaluating the sensitivity of “Fiofio” Cajanus cajan (L. Huth and “Olaudi” Vigna unguiculata (L. Walp to gamma irradiation using morphological markers. Seeds of these legumes were exposed to gamma irradiation at 0, 200, 400, 600, 800 Gy from 60Co source. There was significant effect of gamma irradiation on days to 50% seedling emergence in a dosedependent fashion for “Fiofio” but it did not significantly affect the seedling emergence of “Olaudi”. Germination percentage was not affected concerning the two crops but the survival percentage was significantly reduced as the dose of exposure increased, especially for “Fiofio” variety. Growth and yield traits were increased in plants raised from seeds exposed to 200 Gy of gamma irradiation for both legumes. “Fiofio” plants raised from seeds treated with 400, 600 and 800 Gyhave died after two months, while those of “Olaudi” have presented a retarded growth. Explicitly, exposing the seeds of these legumes to 200 Gy of gamma irradiation could serve as a good springboard for theirimprovement.

  8. Influence de différents traitements de prégermination des graines de Vigna unguiculata (L. Walp. sur les performances germinatives et la tolérance au stress hydrique

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    Boucelha, L.

    2015-01-01

    Full Text Available Influence of different pre-germination treatments of Vigna unguiculata (L. Walp. seeds on germination performance and water stress tolerance. Description of the subject. Priming or hardening is a pregermination treatment. This treatment consists of incorporating an osmotic seed treatment (osmopriming or a hormonal (hormopriming and/or a redehydration (hydropriming treatment. The approach allows the elimination of dormancy, homogenization (synchronization of germination, better growth, earlier flowering and a tolerance to abiotic stresses such as drought and salinity. In this kind of treatment, the seed is soaked and then dehydrated before radicle breakthrough, i.e. during the reversible phase of germination. Thus, the seed can return to its initial stage without any damage. Objectives. In this paper, we aimed to study the consequences of hydropriming and osmopriming (by PEG6000 at 10 and 30% on cowpea seeds (Vigna unguiculata, on germination performance and on the water stress tolerance of plants from these seeds. Method. Vigna unguiculata seeds were hydroprimed, hydroprimed twice or osmoprimed (with PEG6000 10 and 30%. For each treatment, germination performance (germination capacity, speed and the water stress tolerance of the plants were studied. Results. Results showed that increased hardness of the seed allowed a faster, more uniform germination and better growth of both the radicle and aerial parts. We also demonstrated that a double redehydration was more effective in improving these parameters. Conclusions. Application of these pretreatments, adapted according to the plant species, will has the capacity to improve seed germination and crop yield, as well as tolerance to water deficit.

  9. Viabilidade de sementes de feijão macassar tratadas com extrato vegetal e acondicionadas em dois tipos de embalagens = Viability of Vigna unguiculata bean seeds treated with vegetable extract and conditioned in two types of packaging

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    Francisco de Assis Cardoso Almeida

    2009-04-01

    Full Text Available Objetivou-se com o presente trabalho estudar a perda de viabilidade das sementes de duas variedades de Vigna unguiculata, acondicionadas em embalagens de papel e recipiente metálico, armazenados em ambiente não controlado. Após a colheita das sementes, em campos preparados para esta finalidade, as mesmas foram beneficiadas manualmente, tratadas, acondicionadas e armazenadas, para que a cada dois meses fossem avaliadas quanto a sua qualidade fisiológica mediante teste de germinação, indicando a melhor capacidade armazenadora para a condição do estudo. A análise dos dados foi realizada no delineamento inteiramente casualizado, disposto em esquema fatorial 2 x 2 x 2x 6 com 4 repetições, sendo os fatores quantitativos revelados pela regressão na análise de variância. De acordo com os resultados obtidos, a viabilidade das sementes de feijão Vigna unguiculata foi afetada pelos tratamentos e condição do armazenamento, tendo ao final de 360 dias a germinação passada de 99,37 para 41,68%; a variedade Emepa foi superior a Corujinha em 63,47% e o extrato de Piper nigrum revelou-se eficiente na manutenção da viabilidade dessas sementes.The objective of this work was to study the loss of seed viability of two varieties of Vigna unguiculata, conditioned in paper andmetallic packaging, and stored without temperature and relative air humidity control. After seed harvest, in fields prepared for this purpose, the seeds were manually processed, treated, conditioned and stored; every two months, their physiological quality was evaluated using a germination test. The data analysis was carried out using an entirely randomized design, in a 2 x 2 x 2 x 6 factorial scheme with four repetitions; the quantitative factors were disclosed by the regression in the analysis of variance. Based on the results, the viability of the Vigna unguiculata bean seeds was affected by the treatments and storage condition: after 360 days, the germination rate decreased

  10. Genome wide linkage disequilibrium in Chinese asparagus bean (Vigna. unguiculata ssp. sesquipedialis) germplasm: implications for domestication history and genome wide association studies.

    Science.gov (United States)

    Xu, P; Wu, X; Wang, B; Luo, J; Liu, Y; Ehlers, J D; Close, T J; Roberts, P A; Lu, Z; Wang, S; Li, G

    2012-07-01

    Association mapping of important traits of crop plants relies on first understanding the extent and patterns of linkage disequilibrium (LD) in the particular germplasm being investigated. We characterize here the genetic diversity, population structure and genome wide LD patterns in a set of asparagus bean (Vigna. unguiculata ssp. sesquipedialis) germplasm from China. A diverse collection of 99 asparagus bean and normal cowpea accessions were genotyped with 1127 expressed sequence tag-derived single nucleotide polymorphism markers (SNPs). The proportion of polymorphic SNPs across the collection was relatively low (39%), with an average number of SNPs per locus of 1.33. Bayesian population structure analysis indicated two subdivisions within the collection sampled that generally represented the 'standard vegetable' type (subgroup SV) and the 'non-standard vegetable' type (subgroup NSV), respectively. Level of LD (r(2)) was higher and extent of LD persisted longer in subgroup SV than in subgroup NSV, whereas LD decayed rapidly (0-2 cM) in both subgroups. LD decay distance varied among chromosomes, with the longest (≈ 5 cM) five times longer than the shortest (≈ 1 cM). Partitioning of LD variance into within- and between-subgroup components coupled with comparative LD decay analysis suggested that linkage group 5, 7 and 10 may have undergone the most intensive epistatic selection toward traits favorable for vegetable use. This work provides a first population genetic insight into domestication history of asparagus bean and demonstrates the feasibility of mapping complex traits by genome wide association study in asparagus bean using a currently available cowpea SNPs marker platform.

  11. Effect of different home-cooking methods on the bioaccessibility of zinc and iron in conventionally bred cowpea (Vigna unguiculata L. Walp consumed in Brazil

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    Elenilda J. Pereira

    2016-03-01

    Full Text Available Background: The cowpea (Vigna unguiculata L. Wap. is an excellent source of iron and zinc. However, iron from plant sources is poorly absorbed compared with iron from animal sources. Objectives: The objective of this study was to evaluate iron and zinc bioaccessibility in cowpea cultivars after processing. Methods: Zinc and iron bioaccessibilities in cowpea samples were determined based on an in vitro method involving simulated gastrointestinal digestion with suitable modifications. Results: When water-soaked beans were cooked in a regular pan, the highest percentage of bioaccessible iron obtained was 8.92%, whereas when they were cooked in a pressure cooker without previous soaking, the highest percentage was 44.33%. Also, the percentage of bioaccessible zinc was 52.78% when they were cooked in a regular pan without prior soaking. Higher percentages of bioaccessible iron were found when cooking was done in a pressure cooker compared with regular pan cooking. In all cultivars, cooking of cowpea beans in both pressure cooker and in a regular pan yielded higher percentages of bioaccessible zinc compared with availability of bioaccessible iron. Conclusions: Iron bioaccessibility values suggest that cooking in a regular pan did not have a good effect on iron availability, since the percentage of bioaccessible iron was lower than that of zinc. The determination of iron and zinc bioaccessibility makes it possible to find out the actual percentage of absorption of such minerals and allows the development of efficient strategies for low-income groups to access foods with high levels of these micronutrients.

  12. Effect of different home-cooking methods on the bioaccessibility of zinc and iron in conventionally bred cowpea (Vigna unguiculata L. Walp) consumed in Brazil

    Science.gov (United States)

    Pereira, Elenilda J.; Carvalho, Lucia M. J.; Dellamora-Ortiz, Gisela M.; Cardoso, Flávio S. N.; Carvalho, José L. V.

    2016-01-01

    Background The cowpea (Vigna unguiculata L. Wap.) is an excellent source of iron and zinc. However, iron from plant sources is poorly absorbed compared with iron from animal sources. Objectives The objective of this study was to evaluate iron and zinc bioaccessibility in cowpea cultivars after processing. Methods Zinc and iron bioaccessibilities in cowpea samples were determined based on an in vitro method involving simulated gastrointestinal digestion with suitable modifications. Results When water-soaked beans were cooked in a regular pan, the highest percentage of bioaccessible iron obtained was 8.92%, whereas when they were cooked in a pressure cooker without previous soaking, the highest percentage was 44.33%. Also, the percentage of bioaccessible zinc was 52.78% when they were cooked in a regular pan without prior soaking. Higher percentages of bioaccessible iron were found when cooking was done in a pressure cooker compared with regular pan cooking. In all cultivars, cooking of cowpea beans in both pressure cooker and in a regular pan yielded higher percentages of bioaccessible zinc compared with availability of bioaccessible iron. Conclusions Iron bioaccessibility values suggest that cooking in a regular pan did not have a good effect on iron availability, since the percentage of bioaccessible iron was lower than that of zinc. The determination of iron and zinc bioaccessibility makes it possible to find out the actual percentage of absorption of such minerals and allows the development of efficient strategies for low-income groups to access foods with high levels of these micronutrients. PMID:26945231

  13. Sensitivity studies of the common bean (Vigna unguiculata) and maize (Zea mays) to different soil types from the crude oil drilling site at Kutchalli, Nigeria

    Energy Technology Data Exchange (ETDEWEB)

    Anoliefo, G.O. [Dept. of Botany, Univ. of Benin, Benin City (Nigeria); Isikhuemhen, O.S. [Dept. of Natural Resources and Environmental Design, NC Agricultural and Technical State Univ., Greensboro, NC (United States); Ohimain, E.I. [Rohi Biotechnologies Ltd., Port Harcourt (Nigeria)

    2006-02-15

    Background, aims and scope. The economic growth that Nigeria has enjoyed as a result of oil revenue has its drawback through exposure of people in the oil producing areas to environmental contamination, due largely to the increase in the movement of oil. Activities associated with oil well drilling on agricultural lands have led to serious economic losses on the communities affected. The local people in most of these communities are peasants who do not know how to react to drilling wastes or polluted fields where they have their crops. A case under study is the Kutchalli oil drilling area. Methods. Waste pit soil from drilling waste dumps in Kutchalli oil drilling area was tested whole and in combinations with 'clean' soil for their abilities to support plant growth and development in common bean (Vigna unguiculata) and maize (Zea mays). Seed germination, plant height, leaf area, biomass accumulation, respiratory activity as well as soil chemical analysis were used to access the ability of waste pit soil to support plant growth and development in the test plants. Results, discussion and conclusions. Waste pit soil completely inhibited the germination of bean and maize seeds. Waste pit soil in combinations with different proportions of Kutchalli soil gave growth (germination, height of plants, number of leaves, leaf area, etc.) values that were inferior to the control soil (Kutchalli) and the independent control soil (Monguno). Seeds planted in the test soil combinations containing waste pit soil showed significantly low respiratory activity. Waste pit soil seems to be toxic to plant growth and development. Drilling mud in combination with native Kutchalli soil significantly enhanced plant growth and development. Recommendations and outlook. The seed germination, growth and development inhibition by waste pit soil suggests its toxicity. We want to suggest the need for strict control and monitoring of waste pit soil in oil drilling sites. (orig.)

  14. Incidencia y Distribución de Rhizoctonia solani Kühn, en el cultivo de Vigna unguiculata L. Walp. (fríjol caupí

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    Vilma López-Cruz

    2013-01-01

    Full Text Available El trabajo se realizó durante las campañas de siembra 2008-2009 y 2009-2010, en 12 localidades de la provincia de Holguín, en el cultivo de Vigna unguiculata L. Walp (fríjol caupí; con el objetivo de determinar los porcentajes de incidencia y distribución de Rhizoctonia solani Kühn, en cuatro variedades comerciales, 12 localidades y dos campañas de siembra. Los muestreos se llevaron a cabo en campos de 4ha por localidad, en 3 fases fenológicas del cultivo, aplicándose las Metodologías de Señalización y Pronóstico propuestas por el Instituto Nacional de Investigaciones de Sanidad Vegetal (INISAV, 1989. Para evaluar el patógeno se utilizó una escala de daño modificada propuesta por el Centro Internacional de Agricultura Tropical (CIAT, Colombia 1987 y las formulas de Townsend y Hauberger (1963. De los resultados obtenidos se infiere que los porcentajes de distribución y los índices de infección comenzaron a ser superiores a partir de los 30-40 días de establecido el cultivo, encontrándose sus mayores valores a los 60 días para todos los casos, las localidades que alcanzaron los mayores valores de incidencia y distribución del patógeno fueron Arroyo Seco, Boca, Cristino Naranjo y la Guanina y de las variedades evaluadas la var. Titán fue la que mostró mayor tolerancia al ataque del patógeno, seguida por la IITA- Precoz, mientras que la INIFAT-93 y la Carita tradicional fueron las más susceptibles

  15. Effect of Phosphorus Fertilizer and Spacing on Growth, Nodulation Count and Yield of Cowpea (Vigna unguiculata (L Walp in Southern Guinea Savanna Agroecological Zone, Nigeria

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    E. Ndor

    2012-07-01

    Full Text Available The experiments were conducted during 2009 and 2010 rainy season at the research and teaching farm of the college of agriculture, Lafia, Nasarawa state, Nigeria. To determine the effect of phosphorus fertilizer and spacing on growth, nodulation count and yield of cowpea (Vigna unguiculata in southern guinea savanna agroecological zone, Nigeria. The treatments consisted of three levels of phosphorus 0, 20 and 40 kg/ha and three levels of spacing: 15x40, 30x60 and 45x80 cm factorially combined to form nine treatments which were laid in a Randomized Complete Block Design (RCBD and replicated three times to form twenty seven plots. The result showed that Phosphorus fertilizer had a significant (p<0.05 effect on the entire growth and yield parameter assessed in both years. 40 kg/ha of phosphorus gave a significantly higher number of nodulation count/plant (34.95 and 32.24, number of pod/plant (20.64 and 20.24, seed weight/plant (39.56 and 37.64, pods weight/plant (51.45 and 45.31 and seed weight/ha (1.56 and 1.52 t/ha in both years. The spacing also had a significant (p<0.05 response on almost the entire growth and yield parameter assessed except on the weight of 100 seeds. Spacing of 30×60 cm did not differ significantly with 45x80 cm which gave similar result in the no. of pod/plant, seed weight/plant, pods weight/plant and seed weight (t/ha in both years.

  16. Genome wide linkage disequilibrium in Chinese asparagus bean (Vigna. unguiculata ssp. sesquipedialis) germplasm: implications for domestication history and genome wide association studies.

    Science.gov (United States)

    Xu, P; Wu, X; Wang, B; Luo, J; Liu, Y; Ehlers, J D; Close, T J; Roberts, P A; Lu, Z; Wang, S; Li, G

    2012-07-01

    Association mapping of important traits of crop plants relies on first understanding the extent and patterns of linkage disequilibrium (LD) in the particular germplasm being investigated. We characterize here the genetic diversity, population structure and genome wide LD patterns in a set of asparagus bean (Vigna. unguiculata ssp. sesquipedialis) germplasm from China. A diverse collection of 99 asparagus bean and normal cowpea accessions were genotyped with 1127 expressed sequence tag-derived single nucleotide polymorphism markers (SNPs). The proportion of polymorphic SNPs across the collection was relatively low (39%), with an average number of SNPs per locus of 1.33. Bayesian population structure analysis indicated two subdivisions within the collection sampled that generally represented the 'standard vegetable' type (subgroup SV) and the 'non-standard vegetable' type (subgroup NSV), respectively. Level of LD (r(2)) was higher and extent of LD persisted longer in subgroup SV than in subgroup NSV, whereas LD decayed rapidly (0-2 cM) in both subgroups. LD decay distance varied among chromosomes, with the longest (≈ 5 cM) five times longer than the shortest (≈ 1 cM). Partitioning of LD variance into within- and between-subgroup components coupled with comparative LD decay analysis suggested that linkage group 5, 7 and 10 may have undergone the most intensive epistatic selection toward traits favorable for vegetable use. This work provides a first population genetic insight into domestication history of asparagus bean and demonstrates the feasibility of mapping complex traits by genome wide association study in asparagus bean using a currently available cowpea SNPs marker platform. PMID:22378357

  17. Atuação de rizóbios com rizobactéria promotora de crescimento em plantas na cultura do caupi (Vigna unguiculata [L.] Walp. = Rhizobias performance with rhizobacteria growth promoter in plants in the cowpea crop (Vigna unguiculata [L.] Walp.

    Directory of Open Access Journals (Sweden)

    Valéria Nogueira da Silva

    2006-07-01

    Full Text Available O objetivo deste estudo foi verificar a potencialidade da colonização conjunta em feijão caupi (Vigna unguiculata [L.] Walp. por Paenibacillus e Bradyrhizobium em diferentes métodos de inoculação que proporcionam crescimento visando a uma agricultura sustentável. Os trabalhos foram realizados na Empresa Pernambucana de PesquisaAgropecuária (IPA, com Argissolo Amarelo (Itapirema, Estado de Pernambuico, dois modos de inoculação (semente e solo em cultivar de caupi (IPA-205. Foi determinado: número e matéria seca dos nódulos; matéria seca da parte aérea (MSPA e raiz (MSR; relação MSPA/MSR; Nitrogênio acumulado na MSPA; altura da planta. As estirpes de rizóbios apresentaram comportamento instável nos diferentes métodos de inoculação. A eficiência simbiótica da colonização conjunta do sistema radicular por Paenibacillus e as estirpes de Bradyrhizobium nos diferentes métodos de inoculação apresentou-se variável. Os benefícios têm sido aqui evidentes e os fatos informados na literatura podem direcionar futuros estudos que ajudem a explicar os comportamentos acima relatados sobre diferentes mecanismos de ação de cada célula bacteriana.The aim of this work was to verify the cocolonization potential in cowpea bean (Vigna unguiculata [L.] Walp. for Paenibacillus andBradyrhizobium, using different inoculation methods to provide growth, seeking a maintainable agriculture. The study was carried out at Empresa Pernambucana de Pesquisa Agropecuária, in Yellow Argisol (Itapirema, Pernambuco State. Two methods of inoculation (seed and soil in cowpea crop (IPA-205 was used. The following aspects wereevaluated: number and dry matter nodule; shoot dry matter (SDM and root (RDM; relation SDM/RDM; nitrogen accumulated in the SDM and plant height. The rhizobial strains showed low stability in the different inoculation methods. The symbiotic efficiency of the cocolonization for Paenibacillus and the Bradyrhizobium strains in the

  18. Preferência do pulgão-preto, Aphis craccivora Koch, a diferentes genótipos de feijão-de-corda, Vigna unguiculata (L. Walp Black aphid, Aphis craccivora Koch, preference to different cowpea, Vigna unguiculata (L. Walp. cultivars

    Directory of Open Access Journals (Sweden)

    João Gutemberg Leite Moraes

    2007-12-01

    Full Text Available Este trabalho foi desenvolvido visando a avaliar a resposta de cultivares de feijão-de-corda, Vigna unguiculata (L. Walp., à presença do pulgão-preto (Aphis craccivora Koch. Os experimentos foram conduzidos de agosto a outubro de 2004, em casa-de-vegetação da Universidade Federal do Ceará (UFC. As cultivares foram: "Epace-10", "Epace-11", "Patativa", "Pingo de Ouro", "Pitiúba", "BR-10 Piauí", "BR-12 Canindé", "BR-14 Mulato" e "BR-17 Gurguéia". O experimento constou de três ensaios, cada um com cinco tratamentos e seis repetições, sendo o delineamento estatístico inteiramente casualizado. Os genótipos foram cultivados em copos plásticos de 300ml e mantidos em gaiolas protegidas por tela antiafídeos. As plantas foram infestadas após doze dias do plantio através da liberação de cinco fêmeas adultas do pulgão-preto por planta. As avaliações foram realizadas após o terceiro e o quinto dia da infestação, constando da contagem direta das formas adulta e jovem do inseto presentes nas plantas. Os dados obtidos foram analisados através de análise de variância e pelo teste de Tukey em 5% de probabilidade de erro. As cultivares "Epace-10" e "Patativa" foram as menos preferidas por A. craccivora.This research was conducted with the intention of evaluating the response of different cowpea, Vigna unguiculata (L. Walp, cultivars to the black aphid (Aphis craccivora Koch. The bioassays were conducted from August through October of 2004 in a greenhouse at the Ceará Federal University (UFC campus. The cowpea cultivars used were: Epace-10, Epace-11, Patativa, Pingo de Ouro, Pitiúba, BR-10 Piauí, BR-12 Canindé, BR-14 Mulato and BR-17 Gurguéia. Each assay had five treatments and six replications in a completely randomized block design. The genotypes were raised in a 300ml plastic cup and maintained in cages protected by an insect proof net. Plants were infested twelve days after planting with five adult females per plant

  19. Transcriptome Profiling of Two Asparagus Bean (Vigna unguiculata subsp. sesquipedalis) Cultivars Differing in Chilling Tolerance under Cold Stress.

    Science.gov (United States)

    Tan, Huaqiang; Huang, Haitao; Tie, Manman; Tang, Yi; Lai, Yunsong; Li, Huanxiu

    2016-01-01

    Cowpea (V. unguiculata L. Walp.) is an important tropical grain legume. Asparagus bean (V. unguiculata ssp. sesquipedialis) is a distinctive subspecies of cowpea, which is considered one of the top ten Asian vegetables. It can be adapted to a wide range of environmental stimuli such as drought and heat. Nevertheless, it is an extremely cold-sensitive tropical species. Improvement of chilling tolerance in asparagus bean may significantly increase its production and prolong its supply. However, gene regulation and signaling pathways related to cold response in this crop remain unknown. Using Illumina sequencing technology, modification of global gene expression in response to chilling stress in two asparagus bean cultivars-"Dubai bean" and "Ningjiang-3", which are tolerant and sensitive to chilling, respectively-were investigated. More than 1.8 million clean reads were obtained from each sample. After de novo assembly, 88,869 unigenes were finally generated with a mean length of 635 bp. Of these unigenes, 41,925 (47.18%) had functional annotations when aligned to public protein databases. Further, we identified 3,510 differentially expressed genes (DEGs) in Dubai bean, including 2,103 up-regulated genes and 1,407 down-regulated genes. While in Ningjiang-3, we found 2,868 DEGs, 1,786 of which were increasing and the others were decreasing. 1,744 DEGs were commonly regulated in two cultivars, suggesting that some genes play fundamental roles in asparagus bean during cold stress. Functional classification of the DEGs in two cultivars using Mercator pipeline indicated that RNA, protein, signaling, stress and hormone metabolism were five major groups. In RNA group, analysis of TFs in DREB subfamily showed that ICE1-CBF3-COR cold responsive cascade may also exist in asparagus bean. Our study is the first to provide the transcriptome sequence resource for asparagus bean, which will accelerate breeding cold resistant asparagus bean varieties through genetic engineering, and

  20. Transcriptome Profiling of Two Asparagus Bean (Vigna unguiculata subsp. sesquipedalis) Cultivars Differing in Chilling Tolerance under Cold Stress

    Science.gov (United States)

    Tan, Huaqiang; Huang, Haitao; Tie, Manman; Tang, Yi; Lai, Yunsong; Li, Huanxiu

    2016-01-01

    Cowpea (V. unguiculata L. Walp.) is an important tropical grain legume. Asparagus bean (V. unguiculata ssp. sesquipedialis) is a distinctive subspecies of cowpea, which is considered one of the top ten Asian vegetables. It can be adapted to a wide range of environmental stimuli such as drought and heat. Nevertheless, it is an extremely cold-sensitive tropical species. Improvement of chilling tolerance in asparagus bean may significantly increase its production and prolong its supply. However, gene regulation and signaling pathways related to cold response in this crop remain unknown. Using Illumina sequencing technology, modification of global gene expression in response to chilling stress in two asparagus bean cultivars—“Dubai bean” and “Ningjiang-3”, which are tolerant and sensitive to chilling, respectively—were investigated. More than 1.8 million clean reads were obtained from each sample. After de novo assembly, 88,869 unigenes were finally generated with a mean length of 635 bp. Of these unigenes, 41,925 (47.18%) had functional annotations when aligned to public protein databases. Further, we identified 3,510 differentially expressed genes (DEGs) in Dubai bean, including 2,103 up-regulated genes and 1,407 down-regulated genes. While in Ningjiang-3, we found 2,868 DEGs, 1,786 of which were increasing and the others were decreasing. 1,744 DEGs were commonly regulated in two cultivars, suggesting that some genes play fundamental roles in asparagus bean during cold stress. Functional classification of the DEGs in two cultivars using Mercator pipeline indicated that RNA, protein, signaling, stress and hormone metabolism were five major groups. In RNA group, analysis of TFs in DREB subfamily showed that ICE1-CBF3-COR cold responsive cascade may also exist in asparagus bean. Our study is the first to provide the transcriptome sequence resource for asparagus bean, which will accelerate breeding cold resistant asparagus bean varieties through genetic

  1. Transcriptome Profiling of Two Asparagus Bean (Vigna unguiculata subsp. sesquipedalis) Cultivars Differing in Chilling Tolerance under Cold Stress.

    Science.gov (United States)

    Tan, Huaqiang; Huang, Haitao; Tie, Manman; Tang, Yi; Lai, Yunsong; Li, Huanxiu

    2016-01-01

    Cowpea (V. unguiculata L. Walp.) is an important tropical grain legume. Asparagus bean (V. unguiculata ssp. sesquipedialis) is a distinctive subspecies of cowpea, which is considered one of the top ten Asian vegetables. It can be adapted to a wide range of environmental stimuli such as drought and heat. Nevertheless, it is an extremely cold-sensitive tropical species. Improvement of chilling tolerance in asparagus bean may significantly increase its production and prolong its supply. However, gene regulation and signaling pathways related to cold response in this crop remain unknown. Using Illumina sequencing technology, modification of global gene expression in response to chilling stress in two asparagus bean cultivars-"Dubai bean" and "Ningjiang-3", which are tolerant and sensitive to chilling, respectively-were investigated. More than 1.8 million clean reads were obtained from each sample. After de novo assembly, 88,869 unigenes were finally generated with a mean length of 635 bp. Of these unigenes, 41,925 (47.18%) had functional annotations when aligned to public protein databases. Further, we identified 3,510 differentially expressed genes (DEGs) in Dubai bean, including 2,103 up-regulated genes and 1,407 down-regulated genes. While in Ningjiang-3, we found 2,868 DEGs, 1,786 of which were increasing and the others were decreasing. 1,744 DEGs were commonly regulated in two cultivars, suggesting that some genes play fundamental roles in asparagus bean during cold stress. Functional classification of the DEGs in two cultivars using Mercator pipeline indicated that RNA, protein, signaling, stress and hormone metabolism were five major groups. In RNA group, analysis of TFs in DREB subfamily showed that ICE1-CBF3-COR cold responsive cascade may also exist in asparagus bean. Our study is the first to provide the transcriptome sequence resource for asparagus bean, which will accelerate breeding cold resistant asparagus bean varieties through genetic engineering, and

  2. Transcriptome Profiling of Two Asparagus Bean (Vigna unguiculata subsp. sesquipedalis Cultivars Differing in Chilling Tolerance under Cold Stress.

    Directory of Open Access Journals (Sweden)

    Huaqiang Tan

    Full Text Available Cowpea (V. unguiculata L. Walp. is an important tropical grain legume. Asparagus bean (V. unguiculata ssp. sesquipedialis is a distinctive subspecies of cowpea, which is considered one of the top ten Asian vegetables. It can be adapted to a wide range of environmental stimuli such as drought and heat. Nevertheless, it is an extremely cold-sensitive tropical species. Improvement of chilling tolerance in asparagus bean may significantly increase its production and prolong its supply. However, gene regulation and signaling pathways related to cold response in this crop remain unknown. Using Illumina sequencing technology, modification of global gene expression in response to chilling stress in two asparagus bean cultivars-"Dubai bean" and "Ningjiang-3", which are tolerant and sensitive to chilling, respectively-were investigated. More than 1.8 million clean reads were obtained from each sample. After de novo assembly, 88,869 unigenes were finally generated with a mean length of 635 bp. Of these unigenes, 41,925 (47.18% had functional annotations when aligned to public protein databases. Further, we identified 3,510 differentially expressed genes (DEGs in Dubai bean, including 2,103 up-regulated genes and 1,407 down-regulated genes. While in Ningjiang-3, we found 2,868 DEGs, 1,786 of which were increasing and the others were decreasing. 1,744 DEGs were commonly regulated in two cultivars, suggesting that some genes play fundamental roles in asparagus bean during cold stress. Functional classification of the DEGs in two cultivars using Mercator pipeline indicated that RNA, protein, signaling, stress and hormone metabolism were five major groups. In RNA group, analysis of TFs in DREB subfamily showed that ICE1-CBF3-COR cold responsive cascade may also exist in asparagus bean. Our study is the first to provide the transcriptome sequence resource for asparagus bean, which will accelerate breeding cold resistant asparagus bean varieties through genetic

  3. Effet du mode de conservation de l'huile de Jatropha curcas L. sur son efficacité dans la lutte contre les principaux insectes ravageurs du niébé (Vigna unguiculata (L. Walp. au Niger

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    Abdoul Habou, Z.

    2014-01-01

    Full Text Available Influence of the Conservation Mode of Jatropha curcas L. oil on its Efficacy in the Control of Major Insect Pests of Cowpea (Vigna unguiculata (L. Walp in Niger. Jatropha curcas oil has an insecticidal activity harnessed by the farmers in Niger. In this study, we compared the insecticidal activity of two batches of oil conserved during 70 days, one exposed to light and the other kept in the dark. The insecticidal efficacy was evaluated in a field with three concentrations (5, 10 and 15% trial on the main pests of Cowpea (Vigna unguiculata (L. Walp and in a laboratory test on Megalurothrips sjostedti Trybon (Thysanoptera: Thripidae with different concentrations of crude oil (50; 100; 150 and 200 µl. No difference in insecticidal effect was found between the two modes of oil conservation, both in the laboratory and in the field. In the field, regardless of the mode of conservation, the concentrations of 10% of J. curcas oil enables a reduction of over than 80% of thrips, aphids, and bugs compared to the control. Its increased seeds yield more than 50%. The concentration of 15% gives an insecticidal effect comparable to that of the reference treatment (deltaméthrine but induces phytotoxicity symptoms on the leaves of Cowpea.

  4. Description du système racinaire de trois espèces fourragères en zone soudano-sahélienne : Andropogon gayanus, Vigna unguiculata et Stylosanthes hamata

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    Koné D.

    1998-01-01

    Full Text Available Description of root systems of three fodder crops in the Soudano-Sahelian area: Andropogon gayanus, Vigna unguiculata and Stylosanthes hamata. Root systems of fodder crops (Andropogon gayanus, Vigna unguiculata and Stylosanthes hamata were studied at two Research Stations in Mali in 1992, using soil monoliths. In this method, roots are studied throughout the soil profile in cubical compartments of 1 dm3. Root biomass production of A. gayanus planted in 1951 was 4 t.ha-1 against 5 t.ha-1 when planted in 1991. Above-ground biomass was 12 and 8 t.ha-1, respectively. The majority (90% of the root biomass was concentrated in the upper 60 cm of the soil profile even if the root depth reached 180 cm. Root biomass decreased with depth following a negative exponential curve, but root length density decreased to a lesser extent, resulting in increasing specific root length (root length per unit of root biomass with depth. Root biomass of V. unguiculata was 1,118 kg.ha-1 without phosphorus fertilization and 2,922 kg.ha-1 with Pfertilization. The effect of Pon above ground biomass was negligible, and no reduction of specific root length with depth was observed. Root biomass production by S. hamata hardly responded to P fertilization: 3,596 kg.ha-1 without P and 4,161 kg.ha-1 with non-limiting P-supply; above-ground biomass was 8,360 and 10,680 kg.ha-1 respectively. Root length density was relatively high for the whole root profile and specific root length increased from 35 m.g-1 for the 0–20 cm layer to 100 m.g-1 for the 130–140 cm layer, allowing S. hamata to use water and nutrients efficiently throughout the whole profile.

  5. Evaluation of the ionizing radiation effects of the {sup 60}Co on the physical, chemical and nutritional properties of Phaseolus vulgaris L. e Vigna unguiculata (L.) Walp beans; Avaliacao dos efeitos da radiacao ionizante de {sup 60}Co em propriedades fisicas, quimicas e nutricionais dos feijoes Phaseolus vulgaris L. e Vigna unguiculata (L.) Walp

    Energy Technology Data Exchange (ETDEWEB)

    Villavicencio, Anna Lucia Casanas Haasis

    1998-07-01

    The effects of {sup 60} Co ionizing radiations in doses of 0; 0.5; 1.5; 2.5; 5.0 and 10 kGy on beans, Phaseolus vulgaris L., of the carioca variety and Vigna unguiculata (L.) Walp, of the macacar variety stored for 6 months were studied. The cooking time was established, and then the following analyses were carried out: Sensory, vitamins B{sub 1}, B{sub 2} and B{sub 6} protein content, biological evaluation in rats (Food intake and Weight gain (in grams)), apparent Digestibility (Dapp), apparent Net Protein Utilization (NPUapp) and apparent Biological Value (BVapp), as well as the applicability of detection methods of irradiated foodstuffs through germination tests, the analysis of DNA migration, thermoluminescence and analysis of the carbohydrates formed by radiation. Changes in the cooking time were observed for all doses. In doses up to 1 kGy, the nutritional quality of the irradiated beans were not altered. The application of the proposed detection methods of the irradiated foodstuffs allowed the detection of irradiated beans with doses as low as 0.5 kGy. (author)

  6. Bioactive Properties of Phaseolus lunatus (Lima Bean) and Vigna unguiculata (Cowpea) Hydrolyzates Incorporated into Pasta. Residual Activity after Pasta Cooking.

    Science.gov (United States)

    Drago, Silvina R; Franco-Miranda, Hanai; Cian, Raúl E; Betancur-Ancona, David; Chel-Guerrero, Luis

    2016-09-01

    The aims of the study were to study the inclusion of P. lunatus (PLH) and V. unguiculata (VUH) protein hydrolyzates with bioactive properties into a pasta-extruded product and determine residual activity after extrusion or pasta cooking. Both protein hydrolyzates showed angiotensin-converting enzyme inhibition (ACEI) and antioxidant activity (TEAC). PLH showed higher ACEI but lower TEAC than VUH (97.19 ± 0.23 vs. 91.95 ± 0.29 % and 244.7 ± 3.4 vs. 293.7 ± 3.3 μmol Trolox/g, respectively). They were included at 5 or 10 % into wheat pasta. Control pasta had the lowest ACEI activity or TEAC (22.01 ± 0.76 % or 14.14 ± 1.28 μmol Trolox/g, respectively). Higher activity remained in pasta with PLH than VUH after extrusion, and higher the level of addition, higher the ACEI was. Pasta had practically the same ACEI activity after cooking, thus active compounds were not lost by temperature or lixiviation. Regarding TEAC, higher activity remained in pasta with 10 % VUH (31.84 ± 0.17 μmol Trolox/g). Other samples with hydrolyzates had the same activity. After cooking, pasta with hydrolyzates had higher TEAC values than control, but these were not modified by the level of incorporation. Moreover, the profile changed because pasta with PLH had the highest TEAC values (21.39 ± 0.01 and 20.34 ± 0.15 for 5 or 10 % hydrolyzates, respectively). Cooking decreased this activity (~ 20 %), for all samples. Although a certain loss of antioxidant activity was observed, pasta could be a good vehicle for bioactive compounds becoming a functional food. PMID:27422785

  7. Bioactive Properties of Phaseolus lunatus (Lima Bean) and Vigna unguiculata (Cowpea) Hydrolyzates Incorporated into Pasta. Residual Activity after Pasta Cooking.

    Science.gov (United States)

    Drago, Silvina R; Franco-Miranda, Hanai; Cian, Raúl E; Betancur-Ancona, David; Chel-Guerrero, Luis

    2016-09-01

    The aims of the study were to study the inclusion of P. lunatus (PLH) and V. unguiculata (VUH) protein hydrolyzates with bioactive properties into a pasta-extruded product and determine residual activity after extrusion or pasta cooking. Both protein hydrolyzates showed angiotensin-converting enzyme inhibition (ACEI) and antioxidant activity (TEAC). PLH showed higher ACEI but lower TEAC than VUH (97.19 ± 0.23 vs. 91.95 ± 0.29 % and 244.7 ± 3.4 vs. 293.7 ± 3.3 μmol Trolox/g, respectively). They were included at 5 or 10 % into wheat pasta. Control pasta had the lowest ACEI activity or TEAC (22.01 ± 0.76 % or 14.14 ± 1.28 μmol Trolox/g, respectively). Higher activity remained in pasta with PLH than VUH after extrusion, and higher the level of addition, higher the ACEI was. Pasta had practically the same ACEI activity after cooking, thus active compounds were not lost by temperature or lixiviation. Regarding TEAC, higher activity remained in pasta with 10 % VUH (31.84 ± 0.17 μmol Trolox/g). Other samples with hydrolyzates had the same activity. After cooking, pasta with hydrolyzates had higher TEAC values than control, but these were not modified by the level of incorporation. Moreover, the profile changed because pasta with PLH had the highest TEAC values (21.39 ± 0.01 and 20.34 ± 0.15 for 5 or 10 % hydrolyzates, respectively). Cooking decreased this activity (~ 20 %), for all samples. Although a certain loss of antioxidant activity was observed, pasta could be a good vehicle for bioactive compounds becoming a functional food.

  8. Preservation of Cowpea (Vigna unguiculata Seeds: Incidence of Ethanolic Extract from Balanites aegyptiaca, Melia azedarach and Ocimum gratissimum Leaves on Callosobruchus maculatus (Coleptera: Bruchidae

    Directory of Open Access Journals (Sweden)

    Ange-Patrice Takoudjou Miafo

    2014-03-01

    Full Text Available This study aims to evaluate the effect of ethanolic extracts of Balanites aegyptiaca, Melia azedarach and Ocimum gratissimum leaves as bioinsecticide in the preservation of cowpea seeds (Vigna unguiculata against the pest Callosobruchus maculatus. The extracts were obtained by maceration of leaves powdersin ethanol (95%. These extracts were then used to achieve anti-insecticides tests in jars at doses of 10, 15, 25 and 50% compared to a reference compound (Stargrain. The weevils rearing and some biological tests were conducted in laboratory conditions at a temperature of 29.1°C and a relative humidity of 74%. The results showed that these extracts have anti-oviposition activity and ovicidal dose-dependent. Doses 25 and 50% of Melia azedarach and Ocimum gratissimum have completely inhibited the spawning activity of C. maculatus 24 h after treatment. There was significant difference (p<0.05 between oviposition due to the positive control (Stargrain and that due to dose 50% of the three extracts. Compared to the negative control (ethanol 95%, all these plant extracts have significantly reduced (p<0.05 oviposition of C. maculatus female (38 eggs per 100 seeds after 4 days. The dose 10% of B. aegyptiaca and 50% of O. gratissimum showed no weevil emergency. It have been also noticed a reduction in seeds depreciation and rate attack in all the treated settings compared to the control. So the rate attack and the mass loss were proportional to the doses of B. aegyptiaca leaves extract but conversely proportional to doses of Melia azedarach and O. gratissimum leaves extracts. No depreciation of seeds was recorded at doses 10 and 15% of B. aegyptiaca and 50% of O. gratissimum. The different treatments did not affect the germination of seeds; the highest germination rate (93.67% was recorded with seeds treated with the dose 50% of O. gratissimum against only 65% with those treated with the positive control. These ethanolic extracts have shown insecticidal

  9. Physicochemical properties of cowpea (Vigna unguiculata L. Walp. meals and their apparent digestibility in white shrimp (Litopenaeus vannamei Boone Propiedades fisicoquímicas de harinas de frijol yorimón (Vigna unguiculata L. Walp. y su digestibilidad aparente en camarón Litopenaeus vannamei Boone

    Directory of Open Access Journals (Sweden)

    Martha Elisa Rivas-Vega

    2009-04-01

    Full Text Available The effect of different feed processing methods on the physicochemical properties, and apparent digestibility of cowpea (Vigna unguiculata meals as ingredients in diets for white shrimp (Litopenaeus vannamei was investigated. Five experimental cowpea meals were prepared: whole raw (WRC, dehulled (DC, cooked (CC, germinated (GC and extruded (EXC. The physicochemical properties of the meals were evaluated using differential scanning calorimetry. The meals were included at 15% in diets for L. vannamei (15.4 g to determine firmness of pellets and in vivo digestibility of nutrients by using chromic oxide as inert marker. Six diets were evaluated: a control diet, and five diets containing the different cowpea meals. Transition enthalpy significantly decreased after thermal treatment, from 6.1 J/g in WRC to 1.4 J/g in CC, and disappeared in EXC. Firmness of pellets varied from 1.1 N in the EXC diet to 2.8 N in the WRC diet. A significant negative correlation between transition enthalpy and carbohydrate digestibility was found. Dry matter, protein, carbohydrate and lipid digestibility of cowpea meals significantly increased after germinating, cooking or extruding. It is concluded that germinated, cooked and extruded cowpea meals are highly digestible for shrimp and that enthalpy of transition is negatively correlated with the digestibility of carbohydrates.Se evaluó el efecto de diferentes procesos sobre las propiedades fisicoquímicas y digestibilidad aparente de la harina de frijol yorimón (Vigna unguiculata como ingrediente en alimentos para camarón blanco (Litopenaeus vannamei. Se elaboraron cinco harinas de frijol yorimón: entero crudo (WRC, decorticado (DC, cocido (CC, germinado (GC y extruido (EXC. Las características térmicas de las harinas fueron evaluadas usando calorimetría diferencial de barrido. Se elaboraron seis alimentos experimentales: un alimento control y cinco alimentos conteniendo 15% de las diferentes harinas de frijol yorim

  10. Cowpea [Vigna unguiculata (L.) Walp].

    Science.gov (United States)

    Behura, Ratikanta; Kumar, Sanjeev; Saha, Bedabrata; Panda, Manasa Kumar; Dey, Mohitosh; Sadhukhan, Ayan; Mishra, Sagarika; Alam, Shamsher; Sahoo, Debee Prasad; Sugla, Twinkle; Sahoo, Lingaraj

    2015-01-01

    Agrobacterium tumefaciens-mediated transformation is an efficient method for incorporating genes and recovering stable transgenic plants in cowpea because this method offers several advantages such as the defined integration of transgenes, potentially low copy number, and preferential integration into transcriptional active regions of the chromosome. Cotyledonary node explants of cowpea present an attractive target for T-DNA delivery followed by regeneration of shoots via axillary proliferation without involvement of a de novo regeneration pathway. In this chapter, we describe a detailed protocol for Agrobacterium-mediated transformation of the cowpea variety Pusa Komal. The seedling cotyledonary node explants are used for cocultivation with an Agrobacterium strain EHA105 harboring standard binary vector, pCAMBIA2301 or pNOV2819, and putative transformed plants are selected using aminoglycoside antibiotic or mannose as sole carbon source, respectively. The entire process includes explant infection to transgenic seed generation in greenhouse. PMID:25300846

  11. EFFECT OF PHOSPHORUS AND PLANT DENSITY ON THE YIELD COMPONENTS OF COW-PEA (Vigna unguiculata L. Walp: MORFOLOGICAL CHARACTERISTICS EFEITO DO FÓSFORO E DA DENSIDADE DE POPULAÇÃO SOBRE OS COMPONENTES DO RENDIMENTO DO FEIJÃO-DE-CORDA (Vigna unguiculata L. Walp: CARACTERÍSTICAS MORFOLÓGICAS

    Directory of Open Access Journals (Sweden)

    Benedito Carlos Lemos de Carvalho

    2007-09-01

    Full Text Available

    A field experiment was carried out on a distrophic Red Yellow Latosol Text, in the State of Bahia, Brazil, to evaluate the effects of population densities and levels of phosphorous dressing on some morphological characteristics of cow-pea (Vigna unguiculata L Walp, Cv “Tv x 1836-013J”, with a determinate growing habit. Population densities of 40, 80, 160 and 320 thousands of plants per hectare and phosphorus levels at 0, 40, 80 and 160 kg P205 per hectare were applied. Early and late flowering measurements were taken for number of knots on stems and sprouts, foliar area, foliar area index and dry matter production per plant. At harvest date, were taken for number of knots on stems and sprouts. From the observations made at early flowering, only foliar index was affected by plant density. An increment of about 531% was observed on foliar area index by increasing plant density from 40.000 to 320.000 plants per hectare. Data collected at late flowering showed a significant effect of plant density on the number of knots per sprout, total number of knots per plant and foliar area, that diminished with increasing plant density. Phosphorus levels had a significant and positive effect on those variables. However, increasing plant density caused a significant reduction on total dry matter yield per plant and a significant increase on foliar index. At harvest, a significant and negative effect of plant density was observed on the number of stem knots, number of sprout knots and total number of knots per plant, with increasing plant densities for 40.000 to 320.000 plants per hectare.

    Foi conduzido um experimento em Cruz das Almas, Bahia, em solo do tipo latossolo Vermelho Amarelo Àlico Textural, onde foi estudado o efeito das densidades de população (DP de 40.000, 80.000, 160.000 e 320.000 plantas/ha e das doses de f

  12. PEG渗透胁迫下12个豇豆品种萌芽期抗旱性评价%Evaluation of drought resistance of 12 cultivars of Vigna unguiculata under PEG osmotic stress during seed germination

    Institute of Scientific and Technical Information of China (English)

    徐小玉; 张凤银; 李俊芳

    2016-01-01

    以20%聚乙二醇(PEG)溶液为渗透介质模拟干旱胁迫条件,研究12个豇豆品种种子萌发特性及抗旱性.结果表明,20%PEG作用下,除鄂豇豆12号发芽势提高3.9%外,其他供试豇豆品种发芽率、发芽势、发芽指数以及活力指数均有不同程度下降,且萌发期豇豆幼苗生长受到抑制.运用隶属函数法综合评价12个豇豆品种种子萌发期抗旱性,各品种豇豆耐旱级别分别为:1级(耐旱型)为天德一号,2级(较耐旱型)为碧园春贵、鄂豇豆12号,3级(中间型)为海亚特、扬研8号、柳风、扬研2号、柳翠,4级(干旱较敏感型)为早翠,5级(干旱敏感型)为美国地豆、紫魁、矮虎.%The germination characteristics and drought resistance evaluation of 12 cultivars of Vigna unguiculata were studied by simulated drought stress conditions with 20% PEG-6000 solution. Results showed that the germination rate, germination potential, germination index and vigor index decreased except Ejiangdou12 whose germination potential improved with 3.9%, and the growth of V. unguiculata seedlings was inhibited under 20%PEG osmotic stress. Using the membership function method, the results showed that the drought resistance levels of 12 varieties of V. unguiculata during germination were classified as, level 1 (resistant, Tiande1), level 2 (tolerant, Biyuanchungui and Ejiangdou12), level 3 (middle type, Haiyate, Yangyan8, Liufeng, Yangyan2 and Liucui), level 4 (slightly drought sensitive, Zaocui), level 5 (drought sensitive, America Didou, Zikui and Aihu).

  13. Influence du décalage du semis du niébé (Vigna unguiculata (L. Walp par rapport au maïs (Zea mays L. sur la croissance et le rendement du niébé

    Directory of Open Access Journals (Sweden)

    Osiru, DSO.

    2002-01-01

    Full Text Available Effect of Time of Planting Cowpea (Vigna unguiculata (L. Walp Relative to Maize (Zea mays L. on Growth and Yield of Cowpea. Field investigations were carried out for three seasons in two locations of Uganda to examine yield benefits when cowpea and maize are planted under intensive farming conditions. Additive mixtures of cowpea were planted into maize thrice at 2 weekly intervals together with sole crops. Time of introducing cowpea into maize significantly affected both the growth and yield of cowpea. Simultaneous planting generally showed a yield advantage (LER> 1 of the cowpea/ maize intercropping systems irrespective of the cowpea varieties used, but LER declined when time of introducing cowpea into maize was delayed being as low as 0.76 when cowpea was planted four weeks after planting maize. The reduction in the growth and yield of cowpea was due to increased shading from the maize plants especially when cowpea was introduced at the fourth week. Therefore, to achieve yield benefit simultaneous planting of maize and cowpea recommended.

  14. Effects of Cropping System and Cowpea Variety on Symbiotic Potential and Yields of Cowpea (Vigna unguiculata L. Walp and Pearl Millet (Pennisetum glaucum L. in the Sudano-Sahelian Zone of Mali

    Directory of Open Access Journals (Sweden)

    Zoumana Kouyaté

    2012-01-01

    Full Text Available Many cowpea varieties (Vigna unguiculata L. Walp have been proposed by research in Mali. None of these varieties were investigated for their symbiotic potential in terms of root nodulation and mycorrhizal infection. An experiment was conducted at Cinzana Research Station, from 2007 to 2009 with an objective to identify a cowpea variety with high symbiotic potential which may improve millet/cowpea cropping global production. Randomized complete block (RCBD design with a factorial combination of 3 cowpea varieties (IT89KD-374, CZ1-94-23-1, and CZ11-94-5C and 2 cropping systems (millet/cowpea intercropping and cowpea-millet rotation was used. On farm test was conducted to evaluate CZ11-94-5C and IT89KD-374 nodulation performance. Cowpea variety CZ11-94-5-C had the highest nodule number and nodule weight. Millet/cowpea alternate rows intercropping (1/1, only, had a significant influence on cowpea root infection rates by mycorrhizae, on the 45th day after emergence. IT89KD-374 gave the best cowpea grain yield (1540 kg ha−1 in sole crop. The highest millet grain yield (1650 kg ha−1 was obtained under CZ11-94-5C-millet rotation. Farmers' fields assessments results confirmed CZ11-94-5C performance on research station. The CZ11-94-5C cowpea variety needs to be more characterized.

  15. Evaluation on control efficacy of Sprodoptera litura (Fabricius) with 4 kinds of insecticides on Vigna unguiculata%4种杀虫剂防治豇豆斜纹夜蛾的防效比较试验

    Institute of Scientific and Technical Information of China (English)

    秦双; 陈海燕; 潘飞; 吉训聪

    2014-01-01

    通过应用4种药剂防治豇豆斜纹夜蛾药效试验,结果表明,5%氯虫苯甲酰胺悬浮剂防效最好,速效性和持效性都较好,20%氟虫双酰胺水分散粒剂、24%虫螨腈悬浮剂和24%甲氧虫酰肼悬浮剂等药剂也表现出较好的效果,持效期7d以上,生产上防治时可轮换或混合使用。%The efficacies of 4 insecticides were evaluated against Sprodoptera litura (Fabricius) with Vigna unguiculata in field trial. The results showed control efficiency of 5% chlorantraniliprole was the best with rapid efficacy and long efficacy. 20% flubendiamide, 24% chlorfenapyr and 24% methoxyfenozide in the trials had good control effects too, and the effects can be sustained for more than 7 days. These insecticides can be used on the production.

  16. Studies on Physicochemical Properties and Stability of Pigments from purple-red Vigna unguiculata (Linn.)Walp%紫红豇豆色素理化性质及稳定性研究

    Institute of Scientific and Technical Information of China (English)

    隋华嵩; 朱骞; 马海悦; 杨燕; 侯英

    2013-01-01

    In order to provide the basis for the purple-red pigment extraction process and its industrial application, the physicochemical properties of purple -red pigment and effects of preservatives, and pH value, light, temperature, oxidant, reductant and 7 kinds of metal ions on the stability were investigated by using Vigna unguiculata (Linn.)Walp as experimental materials. Results showed that the purple-red pigment from pod and seed of Vigna unguiculata (Linn.)Walp had good water-solubility and was stable to light and heat in acid environment (pH<3), but sensitive to the oxidizer and reducer, pH value of environment (when pH is less than 3 the color is red; when pH is more than 7, the color turn from purple to green). The absorption maximum wavelength of the pigment in the pod and flesh was 270nm and 207nm in the ultraviolet region, respectively, and 542nm and 481nm in the visible wavelength region, respectively. The influences of Ca2+, Na+, Mg2+, K+, Zn2+ were not obvious, but Cu2+ and Fe3+could increase the destruction. The red pigment has good development prospects.%以紫红豇豆为原料,研究了豆荚与种子中紫红色素理化性质以及防腐剂、pH值、光照、温度、还原剂、氧化剂和7种常见金属离子对其稳定性的影响,旨在为紫红豇豆色素的提取加工及工业应用提供理论依据。结果显示:豆荚与种子的紫红色素在紫外区的最大吸收波长分别为270nm和207nm,在可见区的最大吸收波长分别为542nm和481nm,紫红色素水溶性好,颜色随pH值变化而变化(pH<3时颜色鲜红;pH>7时,颜色由紫色转为绿色)。酸性条件(pH<)下该色素对光、低温有很好的稳定性,耐氧化性与耐还原性较强。Ca2+、Na+、Mg2+、K+、Zn2+等离子对紫红色素的稳定性影响不大,Cu2+、Fe3+离子使色素稳定性变差。

  17. Uptake rate of nitrogen from soil and fertilizer, and N derived from symbiotic fixation in cowpea (Vigna unguiculata (L.) Walp.) and common bean (Phaseolus vulgaris L.) determined using the {sup 15}N isotope; Marcha de absorcao do nitrogenio do solo, do fertilizante e da fixacao simbiotica em feijao-caupi (Vigna unguiculata (L.) Walp.) e feijao-comum (Phaseolus vulgaris L.) determinada com uso de {sup 15}N

    Energy Technology Data Exchange (ETDEWEB)

    Brito, Marciano de Medeiros Pereira; Muraoka, Takashi; Silva, Edson Cabral da [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba SP (Brazil)], e-mail: marcianobrito@hotmail.com, e-mail: muraoka@cena.usp.br, e-mail: ecsilva@cena.usp.br

    2009-07-15

    Common bean (Phaseolus vulgaris L.) and cowpea (Vigna unguiculata (L.) Walp.) are among the main sources of plant protein for a large part of the world population, mainly that of low income, and nitrogen is the main constituent of these proteins. The objectives of this study were to evaluate, through the {sup 15}N-dilution technique and using rice and non-nodulating soybean as control plants, the relative contributions of nitrogen sources (symbiotically fixed N, soil native N and fertilizer N) on the growth of common bean and cowpea and to compare the isotopic technique (ID) with the difference methods (DM) for the evaluation of symbiotic N{sub 2} fixation. The study was carried out in a greenhouse of the Center for Nuclear Energy in Agriculture - CENA/USP, Sao Paulo State, Brazil, using 5 kg pots with a Typic Haplustox (Dystrophic Red-Yellow Latosol). The experiment was arranged in completely randomized blocks, with 16 treatments and three replications, in an 8 x 2 factorial design. The treatments were eight sampling times: 7, 24, 31, 38, 47, 58, 68 and 78 days after sowing (DAS) and two crops: common bean and cowpea. An N rate of 10 mg kg{sup -1} soil was used, as urea, enriched with an excess of 10 % of {sup 15}N atoms. Symbiotic N fixation supplied the bean and cowpea plants with the greatest amount of accumulated N, followed, in decreasing order, by soil and fertilizer. The highest rate of N symbiotic fixation was observed at the pre-flowering growth stage of the bean and cowpea plants. After the initial growth stage, 24 DAS, rice and non nodulating soybean were appropriate control plants to evaluate symbiotic N fixation. There was a good agreement between ID and DM, except in the initial growth stage of the crops. (author)

  18. Effect of gamma radiation of {sup 60}Co in the variability of chinese bean [Vigna unguiculata (l.) Walpers] en R{sub 4}M{sub 4}; Efecto de la radiacion gamma de {sup 60}Co en la variabilidad de frijol chino [Vigna unguiculata (l.) Walpers] en R{sub 4}M{sub 4}

    Energy Technology Data Exchange (ETDEWEB)

    Salmeron E, J.; Bueno J, J.E.; Valencia E, F. [CSAEGro, Iguala, Guerrero, Tel and Fax 01 733 (33) 24328 (Mexico); Cervantes S, T. [IREGEP (Mexico); Cruz T, E. De la [ININ, Departamento de Biologia, 52750 La Marquesa, Estado de Mexico (Mexico)]. e-mail: csaegro@prodigy.net.mx

    2007-07-01

    Selection of plants of Chinese bean coming from seed irradiated with gammas of {sup 60}Co in the generation R{sub 4}M{sub 4} (fourth recurrent irradiation, fourth segregate generation) were carried out, taking as selection approaches the plant architecture, the numbers of sheaths for plant, sheath longitude, position of the sheaths, grain size and resistance to plagues and illnesses. 17 lines were selected for grain and 3 lines with fodder characteristics of black grain color were also obtained. (Author)

  19. Effect of Six Different Audio Frequencies on Growth of Cowpea ( Vigna unguiculata ) during Seedling Stage%6种不同声频对豇豆苗期生长影响的研究

    Institute of Scientific and Technical Information of China (English)

    姜仕仁; 黄俊

    2011-01-01

    [目的]研究不同类型、频率特征的声波对豇豆苗期生长的影响.[方法]以古典音乐与昆虫鸣声的混合声波(MI)、杜鹃鸣声、蟋蟀鸣声、单一频率的400 Hz声波和多种频率合成的F5,Fn声波进行试验比较,对豇豆苗高、苗重进行测定,采用EXCEL进行统计分析和多重比较.[结果]经6种不同类型和频率声频处理后,豇豆苗期生长状况均显著优于对照组,表明声波能显著促进株高生长,对豇豆苗期助长效果较好的是400Hz、杜鹃鸣声和蟋蟀鸣声,其次是MI,Fn和F5;与对照组相比,杜鹃鸣声和蟋蟀鸣声处理组在生长期间能显著促进苗株增重.[结论]不同类型、频率特征的声波对豇豆苗期生长都有明显的助长作用,但作用效果有所差异.%[ Objective] The aim was to study the effects of acoustic waves with different types and frequency characteristics on the growth of cowpea (Vigna unguiculata) during seedling stage. [ Method] The insect-music mixed sound (MS), cuckoo acoustic song, cricket acoustic song, single 400 Hz frequency acoustic wave, F5 and Fn acoustic waves composed of different frequencies were designed to investigate the effects on height and weight of cowpea seedling, and experimental data were statistically analyzed and multiple-compared by EXCEL. [ Result ] After treatment by six different types and frequencies of acoustic waves, the growth situations of cowpea were better than control. This indicated that acoustic waves could significantly promote height growth of plant. The treatments with good growth promotion effect included 400 Hz frequency acoustic wave, cuckoo acoustic song and cricket acoustic song, followed by MI, Fn and F5. Cuckoo and cricket acoustic song treatment could promote the weight of cowpea seedling during growth stage. [ Conclusion ] Acoustic waves with different types and frequency characteristics had significant growth-promotion effect on growth of cowpea during seedling stage, but

  20. 盐胁迫对4个品种豇豆种子萌发的影响%Study on the Characteristics of Seed Germination of Four Cowpea (Vigna unguiculata) Varieties Under Salt Stress

    Institute of Scientific and Technical Information of China (English)

    张舟; 邬忠康; 陈志成; 吴文强; 黎茵

    2014-01-01

    以4个品种豇豆(Vigna unguiculata)种子为材料,研究了盐胁迫对豇豆种子萌发的影响.用不同浓度的NaCl溶液进行处理,分析了不同品种豇豆种子的萌发情况,对比了4种豇豆种子的相对发芽率、相对发芽指数和相对盐害指数等指标.结果表明:在盐胁迫条件下,随着盐浓度的增加,不同豇豆品种对盐胁迫的反应有所不同;各品种豇豆种子对低盐胁迫(≤25 mmol/L)有一定的适应性,低盐浓度对发芽率影响较小,但对种子的发芽指数影响较大;盐胁迫对4个品种的盐害指数随着盐浓度的增加而增大,较高浓度的盐胁迫对4种豇豆种子的萌发和生长有较强的抑制作用;在50 mmol/L的盐胁迫下,穗丰9号的盐害指数极显著地低于其它3个品种(p<0.01),而夏美1号的盐害指数高于其它品种(p<0.05).通过各项指标对4种豇豆种子进行综合评价,耐盐能力最强的品种为穗丰9号油青豆角,穗丰5号油白豆角和穗丰8号油青豆角次之,夏美1号白豆角耐盐能力最弱.

  1. Onomatopeyas del chino moderno

    Directory of Open Access Journals (Sweden)

    Qiao Yun

    2016-01-01

    Full Text Available Este artículo estudia brevemente las onomatopeyas del chino moderno en tres grandes aspectos. El primero de ellos estudia la expresión de las onomatopeyas y el amplio rango de significados semánticos en la imitación de voces humanas, sonidos de animales o de la naturaleza y demás. La segunda, los rasgos fonológicos que presentan las onomatopeyas en sus estructuras silábicas, formas reduplicadas, consonantes, vocales, etc. La tercera estudia cómo las onomatopeyas también participan en la formación de términos, donde quedan incluidas las palabras compuestas, los verbos, los adjetivos descriptivos, los adverbios y también, entre otros casos, cómo las onomatopeyas pueden funcionar como verbos.

  2. Viabilidade de sementes de feijão caupi após o tratamento com óleo essencial de citronela (Cymbopogon winterianus Jowitt Viability of Vigna unguiculata bean seeds treated with essential oil of Java grass (Cymbopogon winterianus Jowitt

    Directory of Open Access Journals (Sweden)

    M.V.A. Xavier

    2012-01-01

    Full Text Available O tratamento de sementes com óleos essenciais é um método alternativo que auxilia o manejo integrado de pragas. O objetivo deste trabalho foi avaliar a influência do tratamento de sementes de feijão Vigna unguiculata (L. Walp. com o óleo essencial de citronela (Cymbopogon winterianus Jowitt. Foi avaliado o efeito do óleo essencial de C. winterianus sobre V. unguiculata nas dosagens 20 μL, 15 μL, 10 μL, 5 μL e testemunha. A qualidade fisiológica das sementes foi determinada pela porcentagem de emergência, velocidade de emergência e índice de velocidade de emergência. A análise dos dados foi realizada no delineamento inteiramente casualizado, disposto em esquema fatorial 5 x 2 com cinco repetições. As sementes fumigadas apresentaram diferenças estatísticas entre os parâmetros avaliados em relação à testemunha. O óleo essencial de citronela revelou potencialidade alelopática sobre a germinação de sementes de feijão que variou de acordo com a concentração do óleo.Seed treatment with essential oils is an alternative method tool in integrated pest management. The objective of this study was to evaluate the effect of treating Vigna unguiculata (L. Walp. bean seeds with essential oil of Java grass (Cymbopogon winterianus Jowitt. The effect of C. winterianus essential oil on P. vulgaris was evaluated at levels of 20 μL, 15 μL, 10 μL, 5 μL and control. The physiologic quality of the seeds was determined by percentage emergence, speed emergence and speed emergence index. The data analysis was carried out using an entirely randomized design, in a 5 x 2 factorial scheme with five repetitions. The fumigated bean seeds showed the statistics differences among the analyzed parameters when was compared with the no treated check. The essential oil of Java grass revealed allelopathic potentiality on bean seed germination which varied according to the oil concentration.

  3. Marcha de absorção do nitrogênio do solo, do fertilizante e da fixação simbiótica em feijão-caupi (Vigna unguiculata (L. walp. e feijão-comum (Phaseolus vulgaris L. determinada com uso de 15N Uptake rate of nitrogen from soil and fertilizer, and n derived from symbiotic fixation in cowpea (Vigna unguiculata (L. walp. and common bean (Phaseolus vulgaris L. determined using the 15N isotope

    Directory of Open Access Journals (Sweden)

    Marciano de Medeiros Pereira Brito

    2009-08-01

    Full Text Available O feijão-comum e o feijão-caupi estão entre as principais fontes de proteína vegetal para grande parte da população mundial, sobretudo aquela de baixa renda, e o N é o principal constituinte de proteínas. Os objetivos deste trabalho foram de avaliar, por meio da técnica isotópica e tendo como plantas-controle arroz e soja não nodulante, as contribuições relativas das fontes de N (N2-fixação simbiótica, N-solo e N-fertilizante no desenvolvimento do feijão-comum e caupi ao longo do ciclo e comparar o método isotópico (MI com o método da diferença (MD para avaliação da fixação simbiótica de N2. A pesquisa foi realizada em casa de vegetação no Centro de Energia Nuclear na Agricultura - CENA/USP, utilizando-se vasos com 5 kg de terra de um Latossolo Vermelho-Amarelo distrófico. O delineamento experimental foi o de blocos casualizados com 16 tratamentos e três repetições. Os tratamentos (fatorial 8 x 2 compreenderam oito épocas de coleta: 17, 24, 31, 38, 47, 58, 68 e 78 dias após a semeadura (DAS e duas culturas: feijão-comum e feijão-caupi. Utilizou-se uma dose de 10 mg kg-1 de N no solo, na forma de ureia, enriquecida com 10 % de átomos de 15N em excesso. A fixação simbiótica forneceu a maior parte do N acumulado nas plantas de feijão e caupi, seguida, em ordem decrescente, pelo solo e fertilizante. A maior taxa de fixação simbiótica de N ocorreu a partir da fase de prefloração do feijão e do caupi. Após a fase inicial (24 DAS, o arroz e a soja não nodulante tornaram-se adequadas plantas-controle da fixação simbiótica de N2. Houve boa concordância entre o MI e o MD, exceto nos estádios iniciais das culturas.Common bean (Phaseolus vulgaris L. and cowpea (Vigna unguiculata (L. Walp. are among the main sources of plant protein for a large part of the world population, mainly that of low income, and nitrogen is the main constituent of these proteins. The objectives of this study were to evaluate

  4. 水杨酸对豇豆种子萌发及幼苗生理特性的影响%Effects of Salicylic Acid on Seed Germination and Physiological Characteristics of Vigna unguiculata

    Institute of Scientific and Technical Information of China (English)

    唐丹; 王大平

    2012-01-01

    为在豇豆生产上开发利用SA类物质,以之豇28-2豇豆种子为试材,研究了水杨酸浸种对豇豆种子萌发及幼苗生理特性的影响.结果表明:浓度为50 mg/L的水杨酸处理效果最好,发芽率为96.67%,发芽势为94%,种子中可溶性糖含量为19.47%,叶片中叶绿素含量为2.339 mg/g、脯氨酸含量为58.18mg/g、可溶性蛋白质含量为3.92mg/g,能有效促进豇豆种子的萌发及提高幼苗的抗性.%The effects of SA soaking on seed germination and physiological characteristics of V. unguiculata were studied taking Zhijiang 28-2 as the material to exploit and use SA substance in V. unguiculata production. The results showed that 50 mg/L SA had the best effect. The germination rate was 96. 67%, germination potential was 94%, soluble sugar content in seeds was 19. 47%, the contents of chlorophyll, proline and soluble protein in leaf were 2. 339 mg/g, 58. 18 mg/g and 3. 92 mg/g respectively. It can effectively promote the seed germination and seedlings resistance of V. unguiculata.

  5. Efeitos das culturas de milho (Zea mays, feijão (Phaseolus vulgaris e caupi (Vigna unguiculata na agregação de valor ao cultivo da bananeira 'Terra', em Teolândia, litoral sul da Bahia Effects of corn (Zea mays, beans (Phaseolus vulgaris and cowpea (Vigna unguiculata in addition of values plantains 'Terra', in Teolândia, South Coast of Bahia

    Directory of Open Access Journals (Sweden)

    Marcelo Bezerra Lima

    2005-04-01

    Full Text Available São apresentados resultados pertinentes às culturas do milho (Z. mays, feijão (P. vulgaris e caupi (V. unguiculata consorciados com bananeira 'Terra' (Musa AAB em Teolândia, Litoral Sul da Bahia, em cultivo simultâneo, no estabelecimento da bananeira e sucessivo logo após a colheita do primeiro. Os espaçamentos utilizados foram o 4,0 x 2,0 x 3,0 m, 4,0 x 2,0 x 2,6m e 4,0 x 2,0 x 2,8m em fileira dupla, o que permitiu melhor proveito da área pelas culturas intercalares. O milho, ou o feijão, ou o caupi ocupou apenas as ruas entre as fileiras duplas de bananeiras, ficando livres as ruas formadas pelas fileiras duplas, devido ao seu sombreamento pela bananeira ser mais rápido. O objetivo deste trabalho foi avaliar o desempenho das culturas do milho, feijão e caupi na agregação de valor à bananeira 'Terra', quando consorciadas com esta cultura. Nos melhores tratamentos (T9, T6, T8 e T4, o índice de rentabilidade-IR (receita total ÷ custos alcançou valores expressivos, de 3,36 até 6,68, resultando em efeitos altamente positivos em decorrência das tecnologias e insumos utilizados, o que vai permitir a agregação de um elevado valor à bananeira e, conseqüentemente, uma boa remuneração ao produtor. Estes resultados indicam que, para cada real investido, houve um retorno de R$ 3,36 e R$ 6,68, respectivamente. O IR 0,91 (T2, relativo à cultura do milho, implica que houve prejuízo, o que significa que a receita líquida deste tratamento vai reduzir e não incrementar a receita líquida proporcionada pela cultura da bananeira.Results are presented of a intercropping system of corn (Z. mays, beans (P. vulgaris and cowpea (V. unguiculata with plantains (Musa AAB in Teolandia, South Coast of Bahia region in two successive plantations of these crops, being one simultaneous to the establishment of the plantain and the other right after the first harvest. Plants spacing in double rows were used (4.0 x 2.0 x 3.0 m, 4.0 x 2.0 x 2.6 m and 4

  6. Rôle de l'intensité lumineuse sur les capacités parasitaires d'Eupelmus orientalis Crawford et d'Eupelmus vuilleti Crawford, parasitoïdes des Bruchidae ravageurs de graines de niébé (Vigna unguiculata Walp.

    Directory of Open Access Journals (Sweden)

    Ndoutoume-Ndong A.

    2008-01-01

    Full Text Available Role of light intensity on parasitic capacities of Eupelmus orientalis Crawford and Eupelmus vuilleti Crawford, parasitoids of Bruchidae pests of cowpea (Vigna unguiculata Walp. seeds. In tropical Africa, Callosobruchus maculatus Fabricius is a cowpea pest. After the harvest, the disappearance of one of C. maculatus parasitoids (Eupelmus orientalis Crawford limits the duration of stocks conservation. In this study, we used an experimental model imitating the traditional african granaries taking into account the lack of airtightness and the variability of the internal luminous intensity of the different types of granaries. Three kinds of cages were used: cages with an internal luminous intensity of 380 lux, cages with an internal luminous intensity of 3.5 lux, and some others with an internal luminous intensity of 0.2 lux. Four little holes are pierced on the sides of each cage. Each hole was provided with a trap to catch the insects that escaped. Inside each cage, 20 E. orientalis and Eupelmus vuilleti Crawford nymphs were placed. In adult stage, the insects faced a choice of either to escape, or to stay in the cage and then parasitize available hosts. The trapped individuals as well as those remaining in the cage are sorted out by species. Available hosts in the cage which had parasites are also sorted out. The results show that the E. orientalis females born in the cage hardly parasitize available hosts and escape in majority compared to E. vuilleti which stay in the cages. In 380 lux cages, 19% of E. orientalis females escape compared to 6% of E. vuilleti. This percentage of evasion increases with the darkning of the enclosure. Thus, 62% of E. vuilleti females escape from the cages of 3.5 lux and only 11% escape from the cages of 0.2 lux. The majority of E. orientalis individuals escape right from birth, which therefore explains their disappearance from storage. This escape behaviour constitutes a factor limiting the use of this parasitoid as

  7. Bradyrhizobium vignae sp. nov., a nitrogen-fixing symbiont isolated from effective nodules of Vigna and Arachis.

    Science.gov (United States)

    Grönemeyer, Jann Lasse; Hurek, Thomas; Bünger, Wiebke; Reinhold-Hurek, Barbara

    2016-01-01

    Twenty one strains of symbiotic bacteria from root nodules of local races of cowpea (Vigna unguiculata), Bambara groundnut (Vigna subterranea) and peanuts (Arachis hypogaea) grown on subsistence farmers' fields in the Kavango region of Namibia, were previously characterized as a novel group within the genus Bradyrhizobium. To verify their taxonomic position, the strains were further analysed using a polyphasic approach. 16S rRNA gene sequences were most similar to Bradyrhizobium manausense BR 3351T, with Bradyrhizobium ganzhouense RITF806T being the most closely related type strain in the phylogenetic analysis, and Bradyrhizobium yuanmingense CCBAU 10071T in the ITS sequence analysis. Phylogenetic analysis of concatenated glnII-recA-rpoB-dnaK placed the strains in a highly supported lineage distinct from species of the genus Bradyrhizobium with validly published names; they were most closely related to Bradyrhizobium subterraneum 58 2-1T. The status of the species was validated by results of DNA-DNA hybridization. The combination of phenotypic characteristics from several tests, including carbon source utilization and antibiotic resistance, could be used to differentiate representative strains of species of the genus Bradyrhizobium with validly published names. Novel strain 7-2T induced effective nodules on Vigna subterranea, Vigna unguiculata, Arachis hypogaea and on Lablab purpureus. The DNA G+C content of strain 7-2T was 65.4 mol% (Tm). Based on the data presented, we conclude that these strains represent a novel species for which the name Bradyrhizobium vignae sp. nov. is proposed, with strain 7-2T [LMG 28791T, DSMZ 100297T, NTCCM0018T (Windhoek)] as the type strain. PMID:26463703

  8. Characteristic properties of lipase from cowpea (vigna unguiculata) seedlings

    International Nuclear Information System (INIS)

    Lipase activity was assayed in 4 days old cowpea seedlings using olive oil emulsion stabilized with 10% gum acacia as a substrate. The maximum lipase activity was observed at pH 8.0 with Tris-HCI buffer and at 30 degree C. The pH stability was found in between 7.5-8.5. Lipase activity was fairly stable up to 60 degree C and retaining 80% activity whereas, 26% lipase activity was remaining at 100 degree C within 15 minutes. Lipase activity was slightly increased in the presence of MnC/sub 2/ and decreased by the addition of Triton X 100, Tween 80, ZnCI/sub 2/ and mercaptoethanol. (author)

  9. Development and evaluation of drought resistant mutant germplasm of Vigna unguiculata and Vigna Subterranea

    International Nuclear Information System (INIS)

    The aim of this project was to select cowpea and bambara groundnut plants with improved levels of drought resistance without alteration to the colour of the testa or the growth form. M2 to M5 generation plants were tested. The M2 to M4 plants were evaluated in the wooden boxes in the greenhouse and the field. Six cowpea mutant lines and seven bambara groundnut lines were included in a physiological screening experiment that was conducted in the greenhouse. One cowpea line exhibited high yield under watered conditions, and three under drought stress conditions. Three bambara groundnut mutants yielded more than the parent, and one showed relatively high yield under drought stress. It proved possible to examine mutant plants at the seedling stage in wooden boxes, mature plants were screened in rain-out shelter and physiological traits were distinguished among the tested lines for drought stress. Roots of mature plants were also assessed and variation could be correlated with drought tolerance. Chlorophyll fluorescence was found to be a good predictor of plant performance in drought conditions. (author)

  10. Distribution of Bio-accumulated Cd and Cr in two Vigna species and the Associated Histological Variations

    Directory of Open Access Journals (Sweden)

    Ratheesh Chandra, P.

    2010-04-01

    Full Text Available In nutrient culture experiments, bioaccumulation and anatomical effects of cadmium (CdCl2 - 20μM and chromium (K2Cr2O7 - 600 μM on the structure of root and stem was studied by histochemical and analytical methods in Vigna radiata and Vigna unguiculata. Each metal exerted specific influences on the anatomy of various tissues in root and stem. Histochemical localisation of cadmium and chromium was observed in the stained sections of root and stem. Atomic Absorption Spectrophotometric study revealed maximum accumulation of cadmium and chromium in the root tissue as compared to shoot with significant variation among the species. Abundant occurrences of densely stained deposits of chromium were seen in the root stelar region of V. unguiculata and to a lesser extend in V. radiata. Cadmium accumulation in V. radiata was comparatively more than that of V. unguiculata. The findings also revealed that the accumulation pattern of cadmium and chromium varies between species and hence is species specific.

  11. Métodos para avaliação da qualidade fisiológica de sementes de feijão vigna Methods for evaluation of physiological quality of vigna bean seeds

    Directory of Open Access Journals (Sweden)

    Antonieta Laurinda Francisco Bias

    1999-07-01

    Full Text Available Visando comparar diferentes testes de vigor quanto à avaliação da qualidade fisiológica, quatro lotes de sementes de feijão vigna (Vigna unguiculata W. de duas cultivares ('EPACE-10' e 'IPA-206' foram armazenados por 180 dias (novembro/94 a maio/95, em condições normais de ambiente em Pelotas, RS. Bimestralmente, foram conduzidos testes de germinação, envelhecimento acelerado, condutividade elétrica, frio sem solo, emergência de plântulas em campo e peso de matéria seca da parte aérea das plântulas. A análise e a interpretação dos resultados indicaram que a avaliação da qualidade fisiológica de sementes de feijão vigna deve ser fundamentada no conjunto das informações fornecidas por diferentes testes de vigor. O teste de frio sem solo, dentre os testes estudados, é o que apresenta melhor relação com a emergência das plântulas em campo. O peso de matéria seca da parte aérea das plântulas não é eficiente na separação de lotes de sementes de feijão vigna em diferentes níveis de vigor.The aim of this research was to compare vigor tests for seed quality evaluation. Four seed lots of vigna bean (Vigna unguiculata W. of two cultivares (EPACE-10 and IPA-206, were stored for six months (november/94 to may/95 at natural environmen conditions of Pelotas, RS, Brazil and evaluated at two month intervals, throught the following tests: germination, accelerated aging, cold test without soil, electrical condutivity, seedling dry weight and field emergence tests. The analysis and interpretation of the results showed that the evaluation of the physiological quality of vigna bean seeds needs to be based on different vigor tests. Among the tests, the cold test without soil was the best related with seedling field emergence. The seedling dry weight was not effective to separate vigna bean seed lots in different vigor levels.

  12. [STUDIES IN VITRO INHIBITION OF THE ANGIOTENSIN-CONVERTING ENZYME-I, HYPOTENSIVE AND ANTIHYPERTENSIVE EFFECTS OF PEPTIDE FRACTIONS OF V. UNGUICULATA].

    Science.gov (United States)

    Cú-Cañetas, Trinidad; Betancur Ancona, David; Gallegos Tintoré, Santiago; Sandoval Peraza, Mukthar; Chel Guerrero, Luis

    2015-01-01

    Inhibition of angiotensin-converting enzyme I (ACE-I) in vitro and in vivo from peptide fractions by enzymatic hydrolysis of the Vigna unguiculata protein concentrate was evaluated. Hydrolysis was done with Pepsin-Pancreatin and Flavourzima in two separate systems. The resulting hidrolysates were ultrafiltrated to obtain fractions with different molecular weight. The fractions with better inhibition Flavourzima were size > 1 kDa (> 1 kDa-F) and < 1 kDa (< 1 kDa-F), with an IC50 of 1222.84 and 1098.6 μg/ml respectively. Pepsin-Pancreatin fraction. PMID:26545668

  13. Builders Challenge High Performance Builder Spotlight: Yavapai College, Chino Valley, Arizona

    Energy Technology Data Exchange (ETDEWEB)

    None

    2009-12-22

    Building America Builders Challenge fact sheet on Yavapai College of Chino Valley, Arizona. These college students built a Building America Builders Challenge house that achieved the remarkably low HERS score of -3 and achieved a tight building envelope.

  14. EFFECT OF SOME PLANT EXTRACT AGAINST SEED BORNE INFECTION OF COLLECTOTRICHUM DESTRUCTIVUM ON VIGNA UNIGUCULATA L.

    Directory of Open Access Journals (Sweden)

    Umesh P. Mogle1 and

    2012-06-01

    Full Text Available The cowpea, Vigna unguiculata L. Walp is an ancient food crop, suffering from many fungal diseases. Collectotrichum destructivum is a harmful seed borne pathogen causing disease to the cowpea plant. Control of seed borne infection would be a possible means of reducing losses due to this disease, attempts were made, fungal species isolated from cowpea seeds were used as inocula. The effects of leaf extracts of Argemone mexicana L., Semecarpus anacardium L., Cassia fistula L., Tephrosia purpurea (L. Pers., were evaluated for the control of Collectotrichum destructivum on seeds of cowpea. The seeds were soaked in sterile distilled water extract (10, 20 and 30%, w/v of the leaves for 5, 10 and 15 h. All these plant extracts had significant inhibitory growth effect on the fungal pathogen. Argemone mexicana extract was more effective followed by Semecarpus anacardium, Cassia fistula and Tephrosia purpurea plant extracts and compared favorably with benomyl in the control of the pathogen.

  15. Aspectos de la cortesía verbal en chino y en español

    OpenAIRE

    Li, Xinghua

    2013-01-01

    La presente investigación tiene como objetivo estudiar la importancia de la interacción verbal en la comunicación intercultural. El tema principal es la cortesía verbal en chino y en español. Consiste en conocer cómo funciona la cortesía en las comunicaciones verbales, qué diferencias hay entre el chino y el español. Los resultados sirven para mejorar la comunicación intercultural evitando choques innecesarios.

  16. Process Optimization of Tempeh Protein Isolate from Soybean (Glycine Max Merr and Cowpea (Vigna Unguiculata Mixture

    Directory of Open Access Journals (Sweden)

    Asrul Bahar

    2015-04-01

    Full Text Available Production of ‘tempeh’ (fermented soybean protein isolates in Indonesia is still very low. It is necessary to increase the production with the alternative materials which is make by soybean and cowpea mixture. The utilization of this soybean cake (tempeh as protein isolate raw material is expecting benefits both of soybean and cowpea component which can complement each other. Protein’s content of protein isolates should be a minimum of 90% (db. Therefore, it is necessary to find the precipitation pH and the optimum level of purification in order to get tempeh protein isolate with high protein content. The analytical method used descriptive analysis. The optimal process of tempeh protein isolates from soybean and cowpea mixture conducted with pH 5 and pH 4 precipitation, the oil extraction was carried out at the beginning of the process (before extraction of protein and before the drying stage. The result showed that the tempeh protein isolate of soybean and cowpea mixing have 75.12% (db protein content. It was increasing in 20.67% to 50.16% from previous research.

  17. Association studies and legume synteny reveal haplotypes determining seed size in Vigna unguiculata

    Directory of Open Access Journals (Sweden)

    Mitchell R Lucas

    2013-04-01

    Full Text Available Highly specific seed market classes for cowpea and other grain legumes exists because grain is most commonly cooked and consumed whole. Size, shape, color, and texture are critical features of these market classes and breeders target development of cultivars for market acceptance. Resistance to biotic and abiotic stresses that are absent from elite breeding material are often introgressed through crosses to landraces or wild relatives. When crosses are made between parents with different grain quality characteristics, recovery of progeny with acceptable or enhanced grain quality is problematic. Thus genetic markers for grain quality traits can help in pyramiding genes needed for specific market classes. Allelic variation dictating the inheritance of seed size can be tagged and used to assist the selection of large-seeded lines. In this work we applied SNP genotyping and knowledge of legume synteny to characterize regions of the cowpea genome associated with seed size. These marker-trait associations will enable breeders to use marker based selection approaches to increase the frequency of progeny with large seed. For ~800 samples derived from eight bi-parental populations, QTL analysis was used to identify markers linked to ten trait determinants. In addition, the population structure of 171 samples from the USDA core collection was identified and incorporated into a genome-wide association study which supported more than half of the trait-associated regions important in the bi-parental populations. Seven of the total ten QTL were supported based on synteny to seed size associated regions identified in the related legume soybean. In addition to delivering markers linked to major trait determinants in the context of modern breeding, we provide an analysis of the diversity of the USDA core collection of cowpea to identify genepools, migrants, admixture, and duplicates.

  18. Kenaf (Hibiscus cannabinus) and cowpea (Vigna unguiculata) as sugarcane cover crops

    Science.gov (United States)

    A Louisiana sugarcane field is typically replanted every four years due to declining yields, and, although, it is a costly process, it is both necessary and an opportunity to maximize the financial return during the next four year cropping cycle. Fallow planting systems (FPS) during the fallow perio...

  19. Diversity in 113 cowpea [Vigna unguiculata (L) Walp] accessions assessed with 458 SNP markers.

    Science.gov (United States)

    Egbadzor, Kenneth F; Ofori, Kwadwo; Yeboah, Martin; Aboagye, Lawrence M; Opoku-Agyeman, Michael O; Danquah, Eric Y; Offei, Samuel K

    2014-01-01

    Single Nucleotide Polymorphism (SNP) markers were used in characterization of 113 cowpea accessions comprising of 108 from Ghana and 5 from abroad. Leaf tissues from plants cultivated at the University of Ghana were genotyped at KBioscience in the United Kingdom. Data was generated for 477 SNPs, out of which 458 revealed polymorphism. The results were used to analyze genetic dissimilarity among the accessions using Darwin 5 software. The markers discriminated among all of the cowpea accessions and the dissimilarity values which ranged from 0.006 to 0.63 were used for factorial plot. Unexpected high levels of heterozygosity were observed on some of the accessions. Accessions known to be closely related clustered together in a dendrogram drawn with WPGMA method. A maximum length sub-tree which comprised of 48 core accessions was constructed. The software package structure was used to separate accessions into three groups, and the programme correctly identified varieties that were known hybrids. The hybrids were those accessions with numerous heterozygous loci. The structure plot showed closely related accessions with similar genome patterns. The SNP markers were more efficient in discriminating among the cowpea germplasm than morphological, seed protein polymorphism and simple sequence repeat studies reported earlier on the same collection. PMID:25332852

  20. Kinetics of Thermal Inactivation of Peroxidase and Color Degradation of African Cowpea (Vigna unguiculata) Leaves.

    Science.gov (United States)

    Wawire, Michael; Oey, Indrawati; Mathooko, Francis M; Njoroge, Charles K; Shitanda, Douglas; Hendrickx, Marc

    2016-01-01

    Cowpea leaves form an important part of the diet for many Kenyans, and they are normally consumed after a lengthy cooking process leading to the inactivation of peroxidase (POD) that could be used as an indicator for the potential shelf life of the vegetables. However, color degradation can simultaneously occur, leading to poor consumer acceptance of the product. The kinetics of POD in situ thermal (for thermal treatments in the range of 75 to 100 °C/120 min) inactivation showed a biphasic first-order model, with Arrhenius temperature dependence of the rate constant. The kinetic parameters using a reference temperature (Tref ) of 80 °C were determined for both the heat-labile phase (kref = 11.52 ± 0.95 × 10(-2) min(-1) and Ea of 109.67 ± 6.20 kJ/mol) and the heat-stable isoenzyme fraction (kref = 0.29 ± 0.07 × 10(-2) min(-1) and Ea of 256.93 ± 15.27 kJ/mol). Color degradation (L*, a*, and b* value) during thermal treatment was investigated, in particular as the "a*" value (the value of green color). Thermal degradation (thermal treatments between 55 and 80 °C per 90 min) of the green color of the leaves followed a fractional conversion model and the temperature dependence of the inactivation rate constant can be described using the Arrhenius law. The kinetic parameters using a reference temperature (TrefC = 70 °C) were determined as krefC = 13.53 ± 0.01 × 10(-2) min(-1) and EaC = 88.78 ± 3.21 kJ/mol. The results indicate that severe inactivation of POD (as an indicator for improved shelf life of the cooked vegetables) is accompanied by severe color degradation and that conventional cooking methods (typically 10 min/100 °C) lead to a high residual POD activity suggesting a limited shelf life of the cooked vegetables. PMID:26642260

  1. A novel mutation in TFL1 homolog affecting determinacy in cowpea (Vigna unguiculata).

    Science.gov (United States)

    Dhanasekar, P; Reddy, K S

    2015-02-01

    Mutations in the widely conserved Arabidopsis Terminal Flower 1 (TFL1) gene and its homologs have been demonstrated to result in determinacy across genera, the knowledge of which is lacking in cowpea. Understanding the molecular events leading to determinacy of apical meristems could hasten development of cowpea varieties with suitable ideotypes. Isolation and characterization of a novel mutation in cowpea TFL1 homolog (VuTFL1) affecting determinacy is reported here for the first time. Cowpea TFL1 homolog was amplified using primers designed based on conserved sequences in related genera and sequence variation was analysed in three gamma ray-induced determinate mutants, their indeterminate parent "EC394763" and two indeterminate varieties. The analyses of sequence variation exposed a novel SNP distinguishing the determinate mutants from the indeterminate types. The non-synonymous point mutation in exon 4 at position 1,176 resulted from transversion of cytosine (C) to adenine (A) leading to an amino acid change (Pro-136 to His) in determinate mutants. The effect of the mutation on protein function and stability was predicted to be detrimental using different bioinformatics/computational tools. The functionally significant novel substitution mutation is hypothesized to affect determinacy in the cowpea mutants. Development of suitable regeneration protocols in this hitherto recalcitrant crop and subsequent complementation assay in mutants or over-expressing assay in parents could decisively conclude the role of the SNP in regulating determinacy in these cowpea mutants. PMID:25146839

  2. Genetic Diversity and Population Structure of Cowpea (Vigna unguiculata L. Walp).

    Science.gov (United States)

    Xiong, Haizheng; Shi, Ainong; Mou, Beiquan; Qin, Jun; Motes, Dennis; Lu, Weiguo; Ma, Jianbing; Weng, Yuejin; Yang, Wei; Wu, Dianxing

    2016-01-01

    The genetic diversity of cowpea was analyzed, and the population structure was estimated in a diverse set of 768 cultivated cowpea genotypes from the USDA GRIN cowpea collection, originally collected from 56 countries. Genotyping by sequencing was used to discover single nucleotide polymorphism (SNP) in cowpea and the identified SNP alleles were used to estimate the level of genetic diversity, population structure, and phylogenetic relationships. The aim of this study was to detect the gene pool structure of cowpea and to determine its relationship between different regions and countries. Based on the model-based ancestry analysis, the phylogenetic tree, and the principal component analysis, three well-differentiated genetic populations were postulated from 768 worldwide cowpea genotypes. According to the phylogenetic analyses between each individual, region, and country, we may trace the accession from off-original, back to the two candidate original areas (West and East of Africa) to predict the migration and domestication history during the cowpea dispersal and development. To our knowledge, this is the first report of the analysis of the genetic variation and relationship between globally cultivated cowpea genotypes. The results will help curators, researchers, and breeders to understand, utilize, conserve, and manage the collection for more efficient contribution to international cowpea research. PMID:27509049

  3. Study of phenanthrene utilizing bacterial consortia associated with cowpea (Vigna unguiculata) root nodules.

    Science.gov (United States)

    Sun, Ran; Crowley, David E; Wei, Gehong

    2015-02-01

    Many legumes have been selected as model plants to degrade organic contaminants with their special associated rhizosphere microbes in soil. However, the function of root nodules during microbe-assisted phytoremediation is not clear. A pot study was conducted to examine phenanthrene (PHE) utilizing bacteria associated with root nodules and the effects of cowpea root nodules on phytoremediation in two different types of soils (freshly contaminated soil and aged contaminated soil). Cowpea nodules in freshly-contaminated soil showed less damage in comparison to the aged-contaminated soil, both morphologically and ultra-structurally by scanning electron microscopy. The study of polycyclic aromatic hydrocarbon (PAH) attenuation conducted by high performance liquid chromatography revealed that more PAH was eliminated from liquid culture around nodulated roots than nodule-free roots. PAH sublimation and denaturation gradient gel electrophoresis were applied to analyze the capability and diversity of PAH degrading bacteria from the following four parts of rhizo-microzone: bulk soil, root surface, nodule surface and nodule inside. The results indicated that the surface and inside of cowpea root nodules were colonized with bacterial consortia that utilized PHE. Our results demonstrated that root nodules not only fixed nitrogen, but also enriched PAH-utilizing microorganisms both inside and outside of the nodules. Legume nodules may have biotechnological values for PAH degradation. PMID:25601371

  4. Additive interactions of unrelated viruses in mixed infections of cowpea (Vigna unguiculata L. Walp).

    Science.gov (United States)

    Nsa, Imade Y; Kareem, Kehinde T

    2015-01-01

    This study was carried out to determine the effects of single infections and co-infections of three unrelated viruses on three cowpea cultivars (one commercial cowpea cultivar "White" and 2 IITA lines; IT81D-985 and TVu 76). The plants were inoculated with Cowpea aphid-borne mosaic virus (CABMV), genus Potyvirus, Cowpea mottle virus (CMeV), genus Carmovirus and Southern bean mosaic virus (SBMV), genus Sobemovirus singly and in mixture (double and triple) at 10, 20, and 30 days after planting (DAP). The treated plants were assessed for susceptibility to the viruses, growth, and yield. In all cases of infection, early inoculation resulted in higher disease severity compared with late infection. The virus treated cowpea plants were relatively shorter than buffer inoculated control plants except the IT81D-985 plants that were taller and produced more foliage. Single infections by CABMV, CMeV, and SBMV led to a complete loss of seeds in the three cowpea cultivars at 10 DAP; only cultivar White produced some seeds at 30 DAP. Double and triple virus infections led to a total loss of seeds in all three cowpea cultivars. None of the virus infected IITA lines produced any seeds except IT81D-985 plants co-infected with CABMV and SBMV at 30 DAP with a reduction of 80%. Overall, the commercial cultivar "White" was the least susceptible to the virus treatments and produced the most yield (flowers, pods, and seeds). CABMV was the most aggressive of these viruses and early single inoculations with this virus resulted in the premature death of some of the seedlings. The presence of the Potyvirus, CABMV in the double virus infections did not appear to increase disease severity or yield loss. There was no strong evidence for synergistic interactions between the viruses in the double virus mixtures. PMID:26483824

  5. Ozone phytotoxicity in relation to stress ethylene evolution and stomatal resistance in cowpea (Vigna unguiculata) cultivars

    Energy Technology Data Exchange (ETDEWEB)

    Adepipe, N.O.; Tingey, D.T.

    1979-01-01

    In greenhouse experiments, the ozone sensitivities of three cowpea cultivars differing in growth habits and some physiological traits were determined and related to stress ethylene production and leaf diffusive resistance. The cultivars were more sensitive at the 3-leaf than at the 2-leaf stages of growth. There was no consistent leaf injury at less than 0.50 ..mu..l/l of ozone for 2 hr. At an ozone concentration of 1.0 ..mu..l/l for 2 hr. the cultivar Adzuki exhibited marked leaf injury, amounting to over 80% symptom coverage of the leaf surface while New Era and Ife Brown sustained leaf injury maxima of 58 and 35% respectively. Stress ethylene production did not differ among the cultivars. While ozone increased stomatal diffusive resistance in all cultivars, there were not significant differences among the cultivars.

  6. Pretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] walp.

    Directory of Open Access Journals (Sweden)

    Sadeghipour Omid

    2016-01-01

    Full Text Available Lead (Pb is one of the most important toxic heavy metals that reduce plant growth and development. Therefore, finding compounds that can alleviate the toxic effects of Pb is necessary. Nitric oxide (NO is a signaling molecule that plays important roles in different physiological processes in plants, such as the response to biotic and abiotic stresses. A pot experiment was conducted to study the effects of exogenously applied NO on Pb stress tolerance in cowpea. Seeds were soaked at various concentrations of sodium nitroprusside (SNP; NO donor (0, 0.5 and 1 mM for 20 h, and then sown in pots containing 0 or 200 mg kg-1 Pb (NO32. The obtained results showed that Pb stress significantly reduced the chlorophyll value, relative water content (RWC and net photosynthetic rate, but increased lipid peroxidation, proline content and superoxide dismutase (SOD, catalase (CAT, ascorbate peroxidase (APX and glutathione reductase (GR activities. Nonetheless, different levels of NO significantly lowered Pb toxicity by further increasing SOD, CAT, APX and GR activities as well as proline accumulation. NO pretreatment also raised the chlorophyll value, RWC and net photosynthetic rate but reduced lipid peroxidation. Both NO levels (0.5 and 1 mM were effective in Pb stress tolerance; however, 0.5 mM was more effective. These results indicate that NO pretreatment plays an important role in protecting cowpea plants against Pb toxicity by enhancing antioxidant enzyme activities and proline accumulation.

  7. Variability for dry fodder yield and component traits in cowpea [Vigna unguiculata (L. Walp

    Directory of Open Access Journals (Sweden)

    Olayiwola M.O. 1and P.A.S. Soremi2

    2014-03-01

    Full Text Available Eleven cowpea genotypes were evaluated and significant genotypic effects for most of the traits studied. Number of pods/plants, pod, seed and dry fodder yields were high for phenotypic and genotypic coefficients of variation. High heritability values were recorded for number of pods/plant, 100 seed weight and dry fodder yield. Genetic advance was high for seed, pod and dry fodder yield indicating that the traits could be improved through selection. All traits had high genetic advance as per cent of mean except days to 50% flowering and number of seeds/pods. Dry fodder yield alone had combined high genetic advance and heritability values indicating that this trait has high selection value and considered important in cowpea improvement. IT99K-494-6 and IT98k-573-2-1 that were high for seed and pod yield; and dry fodder yield respectively, may be considered for further improvement.

  8. Genetic divergence in cowpea [Vigna unguiculata (L. Walp] for yield components and seed quality parameters

    Directory of Open Access Journals (Sweden)

    Brahmaiah M, Jhansi Rani K, Sunil N and Keshavulu K

    2014-03-01

    Full Text Available Forty cowpea genotypes were evaluated for 18 quantitative characters to estimate the genetic diversity existing among them by using Mahalanobis D2statistics. The genotypes were grouped into six clusters. The cluster strength varied from single genotype (Clusters III, IV and V to 25 genotypes (Cluster I. Clusteres IV and VI had high inter cluster distance. Clusters II, III and I had maximum 100-seed weight, number of seeds per pod and seed yield respectively. Cluster IV had maximum seedling vigour index, germination per cent, peduncle length, number of clusters per plant and number of primary branches. The genotypes from clusters IV and IV may be inter crossed to obtain high variation.

  9. Relationship between the yield contributing characters in cowpea for grain purpose [vigna unguiculata ( l. Walp

    Directory of Open Access Journals (Sweden)

    R.Usha kumari, K.S .Usharani, R.Suguna, and C.R. Anandakumar

    2010-07-01

    Full Text Available The experiment was conducted using fourteen genotypes of cowpea collected from different states in India to study the nature ofgenotypic association between the eight yield contributing characters and direct and indirect effects of the different characters onyield. The study revealed that the days to maturity, number of branches per plant and number of pods per plant showed positivesignificant correlation with seed yield. Path coefficient analysis exhibited days to fifty per cent flowering, plant height, pods perplant showed positive direct effect on yield except days to maturity, branches per plant, seeds per pod and hundred seed weightwhich showed negative direct effects. The characters plant stand at harvest and pods per plant exhibited moderate direct effects onyield. The implications of the results revealed that selection can be done for days to maturity, number of branches per plant andnumber of pods per plant for improving the grain yield in cowpea.

  10. BREEDING FOR DROUGHT TOLERANCE IN PROGNIES OF COWPEA (Vigna unguiculata (L. Walp

    Directory of Open Access Journals (Sweden)

    Ziyad A Abed

    2014-10-01

    Full Text Available A field experiment was undertaken for two seasons viz spring and autumn of 2013 on farm of Department of field crop science, College of Agriculture university of Baghdad for genetic improvement and screening high yielding cultivars of cowpea under water stress condition. In this study total three progenies i.e.S1, S2, S3 were planted under two period of irrigation. All among the tested progenies S3 proved best in all tested parameters. Highest amount of chlorophyll content (65.20 & 56 was reported from the S3 progeny under period of 10 days irrigation in both seasons. In addition , same progeny have about 0.88 and 0.91 relative water content (RWC, 27 & 36 pod per branch, 13 & 12 seeds per pod and 79.39 & 109.29 gm/plant yield under the period of 10 days irrigation respectively for two seasons. The selection led to increase the effectiveness of physiological and morphological traits, which can be considered as effects of selection criteria on plant yield. Results of the study recommended the use selection methods to for developing cultivars of high vigor.

  11. 3-O-β-D-Glucopyranosyltheobroxide from Aerial Parts of Cowpea (Vigna unguiculata).

    Science.gov (United States)

    Aoi, Arata; Yamashita, Yudai; Gao, Xiquan; Uematsu, Makoto; Ota, Maremichi; Takahashi, Kosaku; Yoshihara, Teruhiko; Matsuura, Hideyuki

    2016-05-01

    Theobroxide has been isolated from culture filtrates of Lasiodiplodia theobromae as a potato tuber-inducing compound. In this study, the metabolism of theobroxide was investigated using cowpea as an experimental model and [2H3-7]theobroxide as a substrate for analyzing a metabolite, which revealed that theobroxide applied exogenously to the roots was converted into 3-O-β-D-glucopyranosyltheobroxide. PMID:27319148

  12. A high yielding short duration cowpea (Vigna unguiculata.L variety ‘Hridya’

    Directory of Open Access Journals (Sweden)

    M.R. Bindu*, Sverup John, G. Suja, M.Indira and T.N. Vilasini

    2011-12-01

    Full Text Available Breeding experiments was conducted at Onattukara Regional Agricultural Research Station, Kayamkulam,Kerala AgriculturalUniversity to evolve a high yielding cowpea variety with synchronized maturity during 2000-09. Local cultivars and releasedvarieties like V-2, CO-3, COVU-358, COVU-8456, V-118 and COVU-623 were used as parents in the programme. A shortduration cowpea variety with synchronized maturity was identified and released as ‘Hridya’ at the state level variety releasecommittee during 2010. This variety was developed by mass selection of the lacal Kuttipayar.The important charecteristics of thevariety are the height is 45 cm, pod colour is green, seed colour is straw, and the 100 seed weight is 4.67g. The productivity ofthe variety is 9.5 q ha-1and the duration is 55 days.

  13. ESTUDIO CRÍTICO DE LAS APROXIMACIONES PEDAGÓGICAS AL VERBO CHINO ESTUDIO CRÍTICO DE LAS APROXIMACIONES PEDAGÓGICAS AL VERBO CHINO

    Directory of Open Access Journals (Sweden)

    Helena Casas-Tost

    2008-07-01

    Full Text Available En el presente artículo se formulan las bases para hacer una propuesta didáctica del verbo chino para hispano hablantes. Para ello antes se contextualiza brevemente la enseñanza del chino en España, se examinan las divergencias entre el verbo chino y el español y se analiza el tratamiento recibido por la categoría verbal china en una parte representativa de la literatura especializada. Tras estas consideraciones, la autora presenta su propuesta centrándose en la definición de verbo, su clasificación y la gramaticalización de las categorías verbales en concreto del aspecto.This article sets the basis for a pedagogical approach to the Chinese verb for Spanish-speaking students. It first briefly gives an overview of the Spanish context in relation to the teaching of Chinese, it then examines the differences between the verb in Chinese and Spanish and analyses the way the Chinese verb is treated in a representative part of the specialised literature. After these considerations, the author makes a proposal for a new pedagogical approach of the teaching of the Chinese verb, focusing on its definition, classification and the grammaticalisation of verbal categories, with special emphasis on the verbal aspect.

  14. Diferencias en conducta agresiva entre adolescentes españoles, chinos y mexicanos

    Directory of Open Access Journals (Sweden)

    María S. Torregrosa

    2010-12-01

    Full Text Available La evidencia empírica ha revelado diferencias culturales en la expresión de la agresividad. El objetivo de este estudio fue analizar las diferencias de agresividad entre estudiantes españoles, mexicanos y chinos de Educación Secundaria. La escala de Conducta Antisocial del Teenage Inventory of Social Skills (TISS fue administrada a 420 españoles, 532 mexicanos y 431 chinos, con edades comprendidas entre los 12 y los 15 años. Los análisis de varianza mostraron que los estudiantes chinos presentaron niveles significativamente más altos de agresividad que los estudiantes españoles y mexicanos, no encontrándose diferencias entre los dos últimos grupos. Este patrón de resultados fue similar por género y edad. Así, todas las submuestras de estudiantes chinos (chicos, chicas, 12-13 años y 14-15 años informaron niveles significativamente más altos de agresividad que sus iguales españoles y mexicanos. Sin embargo, las diferencias encontradas fueron de magnitud pequeña y moderada. Los resultados son discutidos de acuerdo con las dimensiones culturales de individualismo vs. colectivismo y distancia de poder.

  15. First Report of Cucumber mosaic virus Isolated from Wild Vigna angularis var. nipponensis in Korea

    Directory of Open Access Journals (Sweden)

    Mi-Kyeong Kim

    2014-06-01

    Full Text Available A viral disease causing severe mosaic, necrotic, and yellow symptoms on Vigna angularis var. nipponensis was prevalent around Suwon area in Korea. The causal virus was characterized as Cucumber mosaic virus (CMV on the basis of biological and nucleotide sequence properties of RNAs 1, 2 and 3 and named as CMV-wVa. CMV-wVa isolate caused mosaic symptoms on indicator plants, Nicotiana tabacum cv. Xanthi-nc, Petunia hybrida, and Cucumis sativus. Strikingly, CMV-wVa induced severe mosaic and malformation on Cucurbita pepo, and Solanum lycopersicum. Moreover, it caused necrotic or mosaic symptoms on V. angularis and V. radiate of Fabaceae. Symptoms of necrotic local or pin point were observed on inoculated leaves of V. unguiculata, Vicia fava, Pisum sativum and Phaseolus vulgaris. However, CMV-wVa isolate failed to infect in Glycine max cvs. ‘Sorok’, ‘Sodam’ and ‘Somyeong’. To assess genetic variation between CMV-wVa and the other known CMV isolates, phylogenetic analysis using 16 complete nucleotide sequences of CMV RNA1, RNA2, and RNA3 including CMV-wVa was performed. CMV-wVa was more closely related to CMV isolates belonging to CMV subgroup I showing about 85.1–100% nucleotide sequences identity to those of subgroup I isolates. This is the first report of CMV as the causal virus infecting wild Vigna angularis var. nipponensis in Korea.

  16. First Report of Cucumber mosaic virus Isolated from Wild Vigna angularis var. nipponensis in Korea.

    Science.gov (United States)

    Kim, Mi-Kyeong; Jeong, Rae-Dong; Kwak, Hae-Ryun; Lee, Su-Heon; Kim, Jeong-Soo; Kim, Kook-Hyung; Cha, Byeongjin; Choi, Hong-Soo

    2014-06-01

    A viral disease causing severe mosaic, necrotic, and yellow symptoms on Vigna angularis var. nipponensis was prevalent around Suwon area in Korea. The causal virus was characterized as Cucumber mosaic virus (CMV) on the basis of biological and nucleotide sequence properties of RNAs 1, 2 and 3 and named as CMV-wVa. CMV-wVa isolate caused mosaic symptoms on indicator plants, Nicotiana tabacum cv. Xanthi-nc, Petunia hybrida, and Cucumis sativus. Strikingly, CMV-wVa induced severe mosaic and malformation on Cucurbita pepo, and Solanum lycopersicum. Moreover, it caused necrotic or mosaic symptoms on V. angularis and V. radiate of Fabaceae. Symptoms of necrotic local or pin point were observed on inoculated leaves of V. unguiculata, Vicia fava, Pisum sativum and Phaseolus vulgaris. However, CMV-wVa isolate failed to infect in Glycine max cvs. 'Sorok', 'Sodam' and 'Somyeong'. To assess genetic variation between CMV-wVa and the other known CMV isolates, phylogenetic analysis using 16 complete nucleotide sequences of CMV RNA1, RNA2, and RNA3 including CMV-wVa was performed. CMV-wVa was more closely related to CMV isolates belonging to CMV subgroup I showing about 85.1-100% nucleotide sequences identity to those of subgroup I isolates. This is the first report of CMV as the causal virus infecting wild Vigna angularis var. nipponensis in Korea. PMID:25289004

  17. Is the jet-drive flute model able to produce modulated sounds like Flautas de Chinos ?

    CERN Document Server

    Terrien, Soizic; De La Cuadra, Patricio; Fabre, Benoît

    2014-01-01

    Flautas de chinos - prehispanic chilean flutes played during ritual celebrations in central Chile - are known to produce very particular beating sounds, the so-called sonido rajado. Some previous works have focused on the spectral analysis of these sounds, and on the input impedance of the complex resonator. However, the beating sounds origin remains to be investigated. Throughout this paper, a comparison is provided between the characteristics of both the sound produced by flautas de chinos and a synthesis sound obtained through time-domain simulation of the jet-drive model for flute-like instruments. Jet-drive model appears to be able to produce quasiperiodic sounds similar to sonido rajado. Finally, the analysis of the system dynamics through numerical continuation methods allows to explore the production mechanism of these quasiperiodic regimes.

  18. PineApp : Aplicación para Android para el aprendizaje del idioma chino

    OpenAIRE

    Pino Linares, Luis

    2013-01-01

    Desarrollo de una aplicación para Android que ofrece un método fácil para aprender chino mandarín desde el español. Desenvolupament d'una aplicació per a Android que ofereix un mètode fàcil per aprendre xinès mandarí des de l'espanyol.

  19. Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L. A. Rich.

    Directory of Open Access Journals (Sweden)

    Rusama Marubodee

    Full Text Available Vigna vexillata (L. A. Rich. (tuber cowpea is an underutilized crop for consuming its tuber and mature seeds. Wild form of V. vexillata is a pan-tropical perennial herbaceous plant which has been used by local people as a food. Wild V. vexillata has also been considered as useful gene(s source for V. unguiculata (cowpea, since it was reported to have various resistance gene(s for insects and diseases of cowpea. To exploit the potential of V. vexillata, an SSR-based linkage map of V. vexillata was developed. A total of 874 SSR markers successfully amplified single DNA fragment in V. vexillata among 1,336 SSR markers developed from Vigna angularis (azuki bean, V. unguiculata and Phaseolus vulgaris (common bean. An F2 population of 300 plants derived from a cross between salt resistant (V1 and susceptible (V5 accessions was used for mapping. A genetic linkage map was constructed using 82 polymorphic SSR markers loci, which could be assigned to 11 linkage groups spanning 511.5 cM in length with a mean distance of 7.2 cM between adjacent markers. To develop higher density molecular linkage map and to confirm SSR markers position in a linkage map, RAD markers were developed and a combined SSR and RAD markers linkage map of V. vexillata was constructed. A total of 559 (84 SSR and 475 RAD markers loci could be assigned to 11 linkage groups spanning 973.9 cM in length with a mean distance of 1.8 cM between adjacent markers. Linkage and genetic position of all SSR markers in an SSR linkage map were confirmed. When an SSR genetic linkage map of V. vexillata was compared with those of V. radiata and V. unguiculata, it was suggested that the structure of V. vexillata chromosome was considerably differentiated. This map is the first SSR and RAD marker-based V. vexillata linkage map which can be used for the mapping of useful traits.

  20. Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich.

    Science.gov (United States)

    Marubodee, Rusama; Ogiso-Tanaka, Eri; Isemura, Takehisa; Chankaew, Sompong; Kaga, Akito; Naito, Ken; Ehara, Hiroshi; Tomooka, Norihiko

    2015-01-01

    Vigna vexillata (L.) A. Rich. (tuber cowpea) is an underutilized crop for consuming its tuber and mature seeds. Wild form of V. vexillata is a pan-tropical perennial herbaceous plant which has been used by local people as a food. Wild V. vexillata has also been considered as useful gene(s) source for V. unguiculata (cowpea), since it was reported to have various resistance gene(s) for insects and diseases of cowpea. To exploit the potential of V. vexillata, an SSR-based linkage map of V. vexillata was developed. A total of 874 SSR markers successfully amplified single DNA fragment in V. vexillata among 1,336 SSR markers developed from Vigna angularis (azuki bean), V. unguiculata and Phaseolus vulgaris (common bean). An F2 population of 300 plants derived from a cross between salt resistant (V1) and susceptible (V5) accessions was used for mapping. A genetic linkage map was constructed using 82 polymorphic SSR markers loci, which could be assigned to 11 linkage groups spanning 511.5 cM in length with a mean distance of 7.2 cM between adjacent markers. To develop higher density molecular linkage map and to confirm SSR markers position in a linkage map, RAD markers were developed and a combined SSR and RAD markers linkage map of V. vexillata was constructed. A total of 559 (84 SSR and 475 RAD) markers loci could be assigned to 11 linkage groups spanning 973.9 cM in length with a mean distance of 1.8 cM between adjacent markers. Linkage and genetic position of all SSR markers in an SSR linkage map were confirmed. When an SSR genetic linkage map of V. vexillata was compared with those of V. radiata and V. unguiculata, it was suggested that the structure of V. vexillata chromosome was considerably differentiated. This map is the first SSR and RAD marker-based V. vexillata linkage map which can be used for the mapping of useful traits. PMID:26398819

  1. Geometría para futuros profesores de primaria: experiencias con el tangram chino

    OpenAIRE

    Fernández, Teresa

    2003-01-01

    Este artículo tiene como objetivo presentar una experiencia sobre didáctica de la geometría llevada a cabo en la facultad de Ciencias de la Educación de Santiago de Compostela con alumnos de la especialidad de maestro en educación primaria. En ella se utilizó un juego de disección (el tangram chino) como base para la realización de una serie de actividades que tienen un objetivo múltiple: concienciar a los alumnos sobre las ventajas del uso de un material manipulativo para el estudio de propi...

  2. El rumor del nopal chino: construcción institucional y efectos sociales de noticias falsas

    Directory of Open Access Journals (Sweden)

    BRUNO LUTZ

    2012-01-01

    Full Text Available Este artículo es el resultado de una investigación sociológica sobre la noticia falsa que circuló a nivel nacional y en Milpa Alta, al sur de la Ciudad de México. Al examinar las diferentes versiones del rumor periodístico de la importación de nopal chino a México, logramos mostrar cómo se articularon los intereses inmediatos de los agentes colectivos implicados, con temáticas de corte histórico, racial, y económico.

  3. Comportamento hídrico e crescimento do feijão vigna cultivado em solos salinizados Hydric behaviour and growth of cowpea cultivated in salinized soils

    Directory of Open Access Journals (Sweden)

    José B. M. Coelho

    2013-04-01

    Full Text Available A salinização dos solos reduz a capacidade das plantas de absorver água o que, em geral, provoca diminuição na sua taxa de crescimento. As respostas das plantas ao estresse salino são melhor correlacionadas com o potencial osmótico do que com a condutividade elétrica do extrato de saturação do solo. Com o objetivo de avaliar os efeitos do estresse salino no crescimento, evapotranspiração e potencial osmótico foliar do feijoeiro vigna [Vigna unguiculata L. (Walp.] conduziu-se um experimento em casa de vegetação da Universidade Federal Rural de Pernambuco (Recife, PE, Brasil. Os tratamentos constaram de um arranjo fatorial 2 x 4 composto de duas texturas de solo e quatro níveis de salinidade do solo (4, 8 e 12 dS m-1 a 25 ºC além da testemunha sem a adição de sais com cinco repetições. Concluiu-se que a salinidade do solo causa redução no consumo de água, no potencial osmótico foliar, na altura das plantas, no número de folhas e na biomassa seca da parte aérea do feijoeiro vigna.Soil salinization reduces the capacity of plants to absorb water, and in general causes decrease in plant growth. Plant responses to salt stress are better correlated with osmotic potential compared to electrical conductivity of soil saturation extract. In order to evaluate the effect of salt stress on growth, water use and leaf osmotic potential of cowpea [Vigna unguiculata L. (Walp.], an experiment was carried out in a greenhouse of the Federal Rural University of Pernambuco (Recife-PE, Brazil. The Treatments were in a factorial arrangement of 2 x 4, comprising of two soil textures and four levels of soil salinity (4, 8 and 12 dS m-1 at 25 °C, and the control without salt addition with five replications. It was concluded that soil salinity causes reduction in water consumption, leaf osmotic potential, plant height, number of leaves and dry biomass of shoot of cowpea.

  4. Effect of temperature and relative-humidity on the development of Liriomyza sativae Blanchard (Diptera: Agromyzidae) in Vigna unguiculata; Efeito da temperatura e umidade relativa do ar no desenvolvimento de Liriomyza sativae Blanchard (Diptera: Agromyzidae) em Vigna unguiculata

    Energy Technology Data Exchange (ETDEWEB)

    Lima, Tiago C. Costa; Geremias, Leandro D; Parra, Jose R.P. [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil)], e-mail: tcclima@esalq.usp.br, e-mail: geremias@esalq.usp.br, e-mail: jrpparra@esalq.usp.br

    2009-07-01

    This research aimed to study the influence of temperature and relative-humidity (RH) on the development of Liriomyza sativae Blanchard during the egg-adult period, in cowpea, to provide essential information for future biological control projects against the pest. An inverse relation was observed between temperature increase in the range from 15 deg C to 32 deg C and development duration. Larval survival was not affected in the temperature range studied, while a high mortality of pupae was observed at 32 deg C (59.9%). RH did not affect the development time of the immature stages, although it influenced their survival. The lower developmental temperature threshold obtained for the egg-adult period was low (7.3 deg C) when compared with other species of Liriomyza, and was rather low for the larval stage (3.4 deg C ). Based on the thermal requirements for L. sativae, it was possible to estimate the occurrence of 24.5 annual generations at a melon producing region in state of Rio Grande do Norte, Brazil. For laboratory rearing aimed at biological control pest programs, the best rearing conditions are 30 deg C and 50% RH for the larval stage and 90% RH for the pupal stage. (author)

  5. Composição química do feijão caupi (Vigna unguiculata L. Walp, cultivar BRS-Milênio Chemical composition of cowpea (Vigna unguiculata L. Walp, BRS-Milênio cultivar

    Directory of Open Access Journals (Sweden)

    Karoline de Macêdo Gonçalves Frota

    2008-06-01

    Full Text Available O feijão caupi é uma das principais culturas alimentares do Nordeste brasileiro. Para melhorar sua resistência contra pragas e produtividade, a nova cultivar (BRS-Milênio foi obtida por melhoramento genético. Este trabalho objetivou caracterizar o potencial nutricional desta variedade, determinando-se a composição centesimal, o perfil de aminoácidos e ácidos graxos, o conteúdo de minerais e a atividade inibitória de tripsina. A semente contém (g.100 g-1: 24,5 de proteínas; 51,4 de carboidratos; 16,6 de fibra insolúvel e 2,7 de fibra solúvel; 2,6 de cinzas, tendo como principais minerais (mg.100 g-1: ferro - 6,8; zinco - 4,1; manganês - 1,5; fósforo - 510,0 e potássio - 1430,0. O teor de lipídios foi de 2,2%, e seu perfil de ácidos graxos apresenta 29,4% de saturados e 70,7% de insaturados. Foi estimado valor calórico de 323,4 kcal.100 g-1 de semente. Encontrou-se baixa atividade inibitória de tripsina (8,0 UIT.mg-1 de amostra. O perfil de aminoácidos foi favorável ao padrão de referência, exceto pela deficiência de sulfurados, sugerindo a necessidade da combinação desta leguminosa com outras fontes alimentares. Os resultados indicam que o feijão caupi possui atributos desejáveis como altos teores de energia, proteínas, fibras e minerais, baixa atividade inibitória de tripsina e, apesar de reduzido conteúdo lipídico, contém alta proporção de ácidos graxos insaturados.Cowpea is one of the major food cultures of the northeastern region of Brazil. To improve its resistance against plagues and to increase its productivity, a new cultivar (BRS-Milênio was obtained by genetic improvement. This study aimed at determining the proximate composition, amino acid and fatty acid profiles, mineral constituents and trypsin inhibitory activity of this cultivar. The seed presents (g.100 g-1: 24.5 protein, 51.4 carbohydrates, 16.6 insoluble fiber and 2.7 soluble fiber, 2.6 ash; major mineral constituents (mg.100 g-1 were: iron - 6.8, zinc - 4.1, manganese - 1.5, phosphorus - 510.0 and potassium - 1430.0. Lipid content was 2.2%, and its fatty acids profile: 29.4% saturated and 70.7% unsaturated. The caloric value was estimated as 323.4 kcal.100 g-1 of seeds. A low trypsin inhibitory activity was found (8.0 TIU.mg-1 of sample. The amino acid profile is in accordance with the reference amino acid standard, except for the sulfur amino acid deficiency, suggesting the need to encourage the combination of this bean with other food sources. Results suggest that cowpea presents high contents of energy, protein, dietary fiber, minerals and a low trypsin inhibitory activity. Although it contains low amounts of lipids, its composition presents a high unsaturated fatty acid profile.

  6. ¿Nuevos modelos, viejos esquemas? El chino y la didáctica de lenguas extranjeras en el marco de convergencia europea

    OpenAIRE

    Casas-Tost, Helena; Rovira-Esteva, Sara

    2009-01-01

    El objetivo de esta presentación es analizar cómo se han llevado a cabo las diferentes aplicaciones del Marco común europeo de referencia para las lenguas al chino en el contexto español. Se hace un repaso de las experiencias previas de aplicación de marcos o modelos supuestamente universales al chino, se presentan los problemas detectados y una serie de propuestas de mejora.

  7. Assessment of average exposure to organochlorine pesticides in southern Togo from water, maize (Zea mays) and cowpea (Vigna unguiculata).

    Science.gov (United States)

    Mawussi, G; Sanda, K; Merlina, G; Pinelli, E

    2009-03-01

    Drinking water, cowpea and maize grains were sampled in some potentially exposed agro-ecological areas in Togo and analysed for their contamination by some common organochlorine pesticides. A total of 19 organochlorine pesticides were investigated in ten subsamples of maize, ten subsamples of cowpea and nine subsamples of drinking water. Analytical methods included solvent extraction of the pesticide residues and their subsequent quantification using gas chromatography-mass spectrometry (GC/MS). Estimated daily intakes (EDIs) of pesticides were also determined. Pesticides residues in drinking water (0.04-0.40 microg l(-1)) were higher than the maximum residue limit (MRL) (0.03 microg l(-1)) set by the World Health Organization (WHO). Dieldrin, endrin, heptachlor epoxide and endosulfan levels (13.16-98.79 microg kg(-1)) in cowpea grains exceeded MRLs applied in France (10-50 microg kg(-1)). Contaminants' levels in maize grains (0.53-65.70 microg kg(-1)) were below the MRLs (20-100 microg kg(-1)) set by the Food and Agriculture Organization (FAO) and the WHO. EDIs of the tested pesticides ranged from 0.02% to 162.07% of the acceptable daily intakes (ADIs). Population exposure levels of dieldrin and heptachlor epoxide were higher than the FAO/WHO standards. A comprehensive national monitoring programme on organochlorine pesticides should be undertaken to include such other relevant sources like meat, fish, eggs and milk. PMID:19680908

  8. Socio-economic aspects of the intensive growing of cowpeas (vigna unguiculata (L. Walp. in Kano, northern Nigeria

    Directory of Open Access Journals (Sweden)

    P. Van Damme

    1986-01-01

    Full Text Available The costs and benefits of single crop cowpea and cowpea grown in, a traditional mixed cropping system are calculated for the Kano area, in northern Nigeria. If all the technical requirements are met, single crop cowpea is more profitable, on a financial return per unit land basis, than the traditional crop mixture. A one year effort to try and familiarize traditional farmers with a new variety and new production methods is clearly not enough and may even demotivate a number of farmers when the yields, and financial , returns, are low. To ensure that the improved technologies are adopted, it will be necessary to provide a credit programme enabling farmers to purchase the improved inputs, and a marketing structure that guarantees fixed and stable market prices throughout the year.

  9. RESPONSE OF COWPEA (VIGNA UNGUICULATA L. GENOTYPES TO NATIVE SOIL RHIZOBIA FOR NODULATION, YIELD AND SOIL PROPERTIES

    Directory of Open Access Journals (Sweden)

    SONAM BINJOLA

    2013-12-01

    Full Text Available A field experiment was conducted on sandy loam soil, on comparision of both the check varieties, COCP-702 performed significantly better than RC-101 in most of the characters under study. The highest soil microbial biomass carbon, i.e., 234.70 μg g-1, soil available N, i.e., 256.56 kg ha-1 and K, i.e., 205.00 kg ha-1 was shown by check variety COCP-702. PGCP-4 recorded significantly more number of nodules plant-1, i.e., 74.33, N concentration in grain, i.e., 4.76%. PGCP-6 gave significantly more grain yield, i.e., 1893.75 kg ha-1, plant N, i.e., 2.15 %, plant protein content, i.e., 13.43 %. This variety also recorded highest N uptake by grain, i.e., 81.81 kg ha-1 and total plant uptake, i.e., 97.64 kg ha-1. Response of COCP-702, PGCP-4 and PGCP-6 to native soil rhizobia were found to be significantly more than the other cowpea genotypes.

  10. Biopotency of serine protease inhibitors from cowpea (Vigna unguiculata) seeds on digestive proteases and the development of Spodoptera littoralis (Boisduval).

    Science.gov (United States)

    Abd El-latif, Ashraf Oukasha

    2015-05-01

    Serine protease inhibitors (PIs) have been described in many plant species and are universal throughout the plant kingdom, where trypsin inhibitors is the most common type. In the present study, trypsin and chymotrypsin inhibitory activity was detected in the seed flour extracts of 13 selected cultivars/accessions of cowpea. Two cowpea cultivars, Cream7 and Buff, were found to have higher trypsin and chymotrypsin inhibitory potential compared to other tested cultivars for which they have been selected for further purification studies using ammonium sulfate fractionation and DEAE-Sephadex A-25 column. Cream7-purified proteins showed two bands on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) corresponding to molecular mass of 17.10 and 14.90 kDa, while the purified protein from Buff cultivar showed a single band corresponding mass of 16.50 kDa. The purified inhibitors were stable at temperature below 60°C and were active at wide range of pH from 2 to 12. The kinetic analysis revealed noncompetitive type of inhibition for both inhibitors against both enzymes. The inhibitor constant (Ki ) values suggested high affinity between inhibitors and enzymes. Purified inhibitors were found to have deep and negative effects on the mean larval weight, larval mortality, pupation, and mean pupal weight of Spodoptera littoralis, where Buff PI was more effective than Cream7 PI. It may be concluded that cowpea PI gene(s) could be potential insect control protein for future studies in developing insect-resistant transgenic plants. PMID:25524889

  11. Effect of de cortication and roasting on trypsin inhibitors and tannin contents of cowpea (Vigna unguiculata L. Walp) seeds.

    Science.gov (United States)

    Balail, Nasara Gumaa

    2014-06-01

    This study was carried out to evaluate the nutritive value of cowpea seeds. Also to minimize the presence of some anti nutritional factors such as tannin and trypsin inhibitors. It is aimed to determined the best method by which we can reduce the anti nutritional factors. Samples of raw, decorticated and decorticated roasted cowpea seeds were subjected to proximate analysis. The results show that for dry matter (93.63, 94.33 and 95.9), for crude protein (29.18, 31.80 and 29.73), for ash (4.60, 4.0 and 5.52) for fiber (6.22, 3.75 and 4.32) for ether extract (2.30, 2.50 and 1.60) for nitrogen free extract (51.33, 52.28 and 54.73), respectively for raw, decorticated and decorticated roasted seeds. Tannin content percentages were determined using method. The results were (0.76, 0.02 and 0.005), respectively. Trypsin inhibitors were determined using enzymatic method the results were (1.68, 0.74 and 1.36), respectively for raw, decorticated and decorticated roasted cowpea seeds. It is concluded that chemical composition was varied between the treatments. Decorticated seeds gives high level of protein followed by the others. De cortication significantly reduced tannin content by 85%. Roasting significantly decreased trypsin inhibitors by 65%. Processing of cowpea seeds either mechanically or by heat, significantly improve their nutritional value. PMID:26035962

  12. A study on zinc distribution in calcareous soils for cowpea (Vigna Unguiculata L.) and barely ( Hordeum Vulgare L.)

    Science.gov (United States)

    Boroomand, Naser; Maleki, Mohammad Reza

    2010-05-01

    Compared to other cereals, such as wheat and barley cultivars which have low sensitivity to Zn deficiency, cowpea is sensitive to zinc (Zn) deficiency, however it extensively grows even in soils with deficient in Zn. A 8-week greenhouse experiment was conducted to study the response of cowpea and barely to Zn in calcareous soils with different DTPA- Zn. The soil samples were taken from soil surface up to 0.3 m in which their DTPA- Zn ranged from 0.5 to 3.5 mg kg-1. Shoot dry matter, concentration and uptake of Zn were found to be significantly correlated with soil DTPA- Zn in cowpea and barely. Critical deficiency level of Zn in cowpea was 1.3 mg kg-1 in soil and 28.5 mg kg-1 in shoot dry matter, however, to barely symptoms of Zn deficiency was not observed and concentration of Zn was higher than the critical level reported in literatures. Organic carbon (OC), calcium carbonate equivalent (CCE), pH and field capacity soil moisture content(FC) were significantly correlated with plant responses to Zn which were the most influenced characteristics to Zn uptake by plants.

  13. Using violet laser-induced chlorophyll fluorescence emission spectra for crop yield assessment of cowpea (Vigna unguiculata (L) Walp) varieties

    Science.gov (United States)

    Anderson, Benjamin; Buah-Bassuah, Paul K.; Tetteh, Jonathan P.

    2004-07-01

    The use of violet laser-induced chlorophyll fluorescence (LICF) emission spectra to monitor the growth of five varieties of cowpea in the University of Cape Coast Botanical Garden is presented. Radiation from a continuous-wave violet laser diode emitting at 396 nm through a fibre is closely incident on in vivo leaves of cowpea to excite chlorophyll fluorescence, which is detected by an integrated spectrometer with CCD readout. The chlorophyll fluorescence spectra with peaks at 683 and 731 nm were used for growth monitoring of the cowpea plants over three weeks and analysed using Gaussian spectral functions with curve fitted parameters to determine the peak positions, area under the spectral curve and the intensity ratio F683/F731. The variation in the intensity ratio of the chlorophyll bands showed sensitive changes indicating the photosynthetic activity of the cowpea varieties. A discussion of the fluorescence result as compared to conventional assessment is presented with regard to discrimination between the cowpea varieties in terms of crop yield performance.

  14. Bowman-Birk protease inhibitor from Vigna unguiculata seeds enhances the action of bradykinin-related peptides.

    Science.gov (United States)

    da Cunha Morales Álvares, Alice; Schwartz, Elisabeth Ferroni; Amaral, Nathalia Oda; Trindade, Neidiane Rosa; Pedrino, Gustavo Rodrigues; Silva, Luciano Paulino; de Freitas, Sonia Maria

    2014-01-01

    The hydrolysis of bradykinin (Bk) by different classes of proteases in plasma and tissues leads to a decrease in its half-life. Here, Bk actions on smooth muscle and in vivo cardiovascular assays in association with a protease inhibitor, Black eyed-pea trypsin and chymotrypsin inhibitor (BTCI) and also under the effect of trypsin and chymotrypsin were evaluated. Two synthetic Bk-related peptides, Bk1 and Bk2, were used to investigate the importance of additional C-terminal amino acid residues on serine protease activity. BTCI forms complexes with Bk and analogues at pH 5.0, 7.4 and 9.0, presenting binding constants ranging from 103 to 104 M-1. Formation of BTCI-Bk complexes is probably driven by hydrophobic forces, coupled with slight conformational changes in BTCI. In vitro assays using guinea pig (Cavia porcellus) ileum showed that Bk retains the ability to induce smooth muscle contraction in the presence of BTCI. Moreover, no alteration in the inhibitory activity of BTCI in complex with Bk and analogous was observed. When the BTCI and BTCI-Bk complexes were tested in vivo, a decrease of vascular resistance and consequent hypotension and potentiating renal and aortic vasodilatation induced by Bk and Bk2 infusions was observed. These results indicate that BTCI-Bk complexes may be a reliable strategy to act as a carrier and protective approach for Bk-related peptides against plasma serine proteases cleavage, leading to an increase in their half-life. These findings also indicate that BTCI could remain stable in some tissues to inhibit chymotrypsin or trypsin-like enzymes that cleave and inactivate bradykinin in situ. PMID:25361421

  15. Studies on hybrid vigour and combining ability for seed yield and contributing characters in cowpea (Vigna unguiculata

    Directory of Open Access Journals (Sweden)

    R.Ushakumari, N.Vairam, C.R. Anandakumar and N. Malini

    2010-07-01

    Full Text Available The combining ability aids in better selection of parents besides elucidating the nature and magnitude of gene action. Heterosisand combining ability analysis were carried out in line x tester model using five lines viz., Kanakamany, Subadra, TC 49-1,Lola and Sarika and five testers viz., CO2, CO4, CO6, CO (CP 7 and VBN 1. The results reveled that TC 49-1, Lola, Sarika,VBN1, CO2 and CO (CP7 were found to be good general combiners for seed yield. Among the parents, TC 49-1, Lola andVBN1 were found good general combiners for days to 50% flowering, plant height, cluster / plant, pods, / plant, length of podand number of seeds / plant. The crosses Lola x VBN 1, Sarika x VBN 1 and Sarika x CO (CP 7 were the best specificcombination for grain yield. The two crosses Lola x VBN 1 and Sarika x CO 4 showed significant heterosis over the standardlatest variety CO (CP7 for seed / pod, cluster / plant, pods / plant and 50% flowering. The crosses TC 49-1 x CO 2 showedhigh heterosis over standard variety for plant height and clusters/plant. Hence, these hybrids can be utilized for commercialcultivation.

  16. Field studies of sweet potatoes and cowpeas in response to elevated carbon dioxide. Progress report. [Ipomoea batatas; Vigna unguiculata

    Energy Technology Data Exchange (ETDEWEB)

    Biswas, P.K.

    1985-01-01

    The physiological and biochemical effects of enriched CO/sub 2/ on sweet potatoes and cowpeas were studied in open top chambers. The main task was to assemble equipment to generate test atmospheres of CO/sub 2/ in the field, using open top chambers as the basic exposure unit for studying the response of sweet potatoes and cowpeas to enriched CO/sub 2/. Plant responses to CO/sub 2/ in open top chambers have been demonstrated by several investigators in a variety of crops. This study focused on growth and development of both sweet potatoes and cowpeas at levels of CO/sub 2/ ranging from the ambient level of 354 ppM to 659 ppM. The effects of CO/sub 2/ on leaf and stem weights, stem length, leaf area, and stomatal number and conductance were studied on both species. Additional studies on sweet potatoes included the effects of CO/sub 2/ on the weight, chemical content and quality of tubers. Additional studies on cowpeas included the effects of CO/sub 2/ on the weight of seeds and rate of nitrogen fixation. 27 figs., 35 tabs. (ACR)

  17. Physical, proximate, functional and pasting properties of flour produced from gamma irradiated cowpea (Vigna unguiculata, L. Walp)

    Science.gov (United States)

    Darfour, B.; Wilson, D. D.; Ofosu, D. O.; Ocloo, F. C. K.

    2012-04-01

    Cowpeas are leguminous seeds widely produced and consumed in most developing countries of sub Saharan Africa. The aim of this study was to determine the physical, proximate, functional and pasting properties of flour obtained from gamma irradiated cowpea. Four cowpea cultivars were irradiated with gamma radiation at dose levels of 0.25, 0.5, 0.75, 1.0 and 1.5 kGy with the unirradiated cultivars serving as controls. The samples were hammer milled, sieved and stored at 4 °C for analysis. Physical, proximate, functional, pasting properties were determined using appropriate methods. In general, the irradiation dose applied to cowpea for insect control did not significantly affect the physical and proximate properties of the flour. However, significant increase (p<0.05) was achieved in paste bulk density, water and oil absorption capacities, foam capacities and least gelation concentrations of flour in general, which may be attributed to the irradiation. The radiation reduced the swelling power and water solubility index significantly. The peak temperature, peak viscosity and setback viscosity of the pastes were significantly (p<0.05) reduced while breakdown viscosity was significantly (p<0.05) increased by the radiation. It was established that the doses used on cowpea affected both the functional and pasting properties of the flour.

  18. Genetic control and transgressive segregation of zinc, iron, potassium, phosphorus, calcium, and sodium accumulation in cowpea (Vigna unguiculata) seeds.

    Science.gov (United States)

    Fernandes Santos, C A; Boiteux, L S

    2015-01-01

    Cowpea crop, through combining a range of essential minerals with high quality proteins, plays an important role in providing nutritional security to human population living in semi-arid regions. Studies on genetics of biofortification with essential minerals are still quite scarce, and the major objective of the present study was to provide genetic information on development of cowpea cultivars with high seed mineral contents. Genetic parameters heritability and minimum number of genes were estimated for seed accumulation of zinc (Zn), iron (Fe), calcium (Ca), phosphorus (P), potassium (K), and sodium (Na). Generation mean and variance analyses were conducted using contrasting parental lines, F₁, F₂, and backcross populations derived from IT97K-1042-3 x BRS Tapaihum and IT97K-1042-3 x Canapu crosses. High narrow-sense heritability (h²) values were found for accumulation of Fe (65-86%), P (74-77%), and K (77-88%), whereas moderate h(2) values were observed for accumulation of Ca (41-56%), Zn (51-83%), and Na (50-55%) in seeds. Significant additive genetic effects as well as parental mean effects were detected in both crosses for all minerals, whereas epistasis was important genetic component in Zn content. The minimum number of genes controlling the accumulation of minerals ranged from two (K) to 11 (P). Transgressive segregation was observed in F2 populations of both crosses for all minerals analyzed. The results suggest that, although under either oligogenic or polygenic control, the seed content of these six minerals in cowpea can be improved via standard breeding methods largely used for self-pollinated crops. PMID:25729958

  19. Preservation of Cowpea (Vigna unguiculata (L.) Walp) Grains against Cowpea Bruchids (Callosobruchus maculatus) Using Neem and Moringa Seed Oils

    OpenAIRE

    Ilesanmi, Joana O.; Daniel T. Gungula

    2010-01-01

    This study was conducted to determine the effects of neem (Azadirachta indica A. Juss) and moringa (Moringa oleifera) seed oils on the storability of cowpea grain. Cowpea samples were treated with various concentrations (0.5, 1.0, and 1.5 mL/200 g cowpea) of pure neem and moringa oils and their mixtures in ratios of 1:1, 1:2, and 1:3. The treated cowpea samples were stored for 180 days. Data were collected every 30 days on number of eggs laid, total weevil population, and percentage of uninfe...

  20. Transformation of Cowpea Vigna unguiculata with a Full-Length DNA Copy of Cowpea Mosaic Virus M-RNA

    NARCIS (Netherlands)

    Hille, Jacques; Goldbach, Rob

    1987-01-01

    A full-length DNA copy of the M-RNA of cowpea mosaic virus (CPMV), supplied with either the 35S promoter from cauliflower mosaic virus (CaMV) or the nopaline synthase promoter from Agrobacterium tumefaciens, was introduced into the T-DNA region of a Ti-plasmid-derived gene vector and transferred to

  1. Genetical analysis of the induced variation by gamma radiation in quantitative characters of Caupi [Vigna unguiculata (L.) Walp.

    International Nuclear Information System (INIS)

    Genetical analysis procedures of the cobalt 60 gamma radiation effects in the induced mutations in quantitative characters of Caupi BR-1 Poty. The following characters were evaluated: day to first flower (FI), number of pods per plant (NVP), pod lenght (CMV), number of suds per pod (NSV), 100 seed wright (PCS), seed yield per plant (PSP) and seed yield per plant estimated by yield components (PSPE). The resistance of irradiated populations to cowpea aphid-borne mosaic virus (CpAMV)was also evaluated. (L.M.J.)

  2. Bowman-Birk Protease Inhibitor from Vigna unguiculata Seeds Enhances the Action of Bradykinin-Related Peptides

    Directory of Open Access Journals (Sweden)

    Alice da Cunha M. Álvares

    2014-10-01

    Full Text Available The hydrolysis of bradykinin (Bk by different classes of proteases in plasma and tissues leads to a decrease in its half-life. Here, Bk actions on smooth muscle and in vivo cardiovascular assays in association with a protease inhibitor, Black eyed-pea trypsin and chymotrypsin inhibitor (BTCI and also under the effect of trypsin and chymotrypsin were evaluated. Two synthetic Bk-related peptides, Bk1 and Bk2, were used to investigate the importance of additional C-terminal amino acid residues on serine protease activity. BTCI forms complexes with Bk and analogues at pH 5.0, 7.4 and 9.0, presenting binding constants ranging from 103 to 104 M−1. Formation of BTCI-Bk complexes is probably driven by hydrophobic forces, coupled with slight conformational changes in BTCI. In vitro assays using guinea pig (Cavia porcellus ileum showed that Bk retains the ability to induce smooth muscle contraction in the presence of BTCI. Moreover, no alteration in the inhibitory activity of BTCI in complex with Bk and analogous was observed. When the BTCI and BTCI-Bk complexes were tested in vivo, a decrease of vascular resistance and consequent hypotension and potentiating renal and aortic vasodilatation induced by Bk and Bk2 infusions was observed. These results indicate that BTCI-Bk complexes may be a reliable strategy to act as a carrier and protective approach for Bk-related peptides against plasma serine proteases cleavage, leading to an increase in their half-life. These findings also indicate that BTCI could remain stable in some tissues to inhibit chymotrypsin or trypsin-like enzymes that cleave and inactivate bradykinin in situ.

  3. Varietal Response of Cowpea (Vigna unguiculata (L. Walp. to Striga gesnerioides (Willd. Vatke Race SG5 Infestation

    Directory of Open Access Journals (Sweden)

    Jean Baptiste NOUBISSIE TCHIAGAM

    2010-09-01

    Full Text Available The parasitic plant Striga gesnerioides (Willd Vatke is becoming a serious threat to cowpea production in Sub-Saharan Africa. At least 7 races of S. gesnerioides exhibiting specific virulence have been identified. The objectives of this study were to identify sources of resistance to S. gesnerioides race SG5 spreads in northern Cameroon and to study the inheritance of the resistance. Twelve accessions of cowpea, IT98K-205-8, IT98K-503-1, IT98K-1092-1, IT99K-216-1-1, IT98K-494-6, IT99K-529-4, IT99K-573-1-1, IT99K-573-2-1, IT00K-1207, IT03K-378-4, IT04K-227-4 and VYA were assessed in field trials for resistance to S. gesnerioides race SG5 in Maroua, northern Cameroon and the genetics of the resistance from two sources was elucidated in artificially infested pots. Data on resistant and susceptible cowpea plants were analyzed using the chi-square test to ascertain the goodness of fit to different genetic ratios. Tests of resistance using field screening techniques showed that among these varieties, four were susceptible, four tolerant, two partially resistant while IT99K-573-1-1 and IT98K-205-8 appeared completely resistant to Striga race SG5. On susceptible varieties, the mean number of emerged S. gesnerioides plants 9 weeks after sowing was 1.8 to 2.4 per cowpea plant, while completely resistant lines carried none. The cowpea genotypes showed significant differences for percentage of infected plants, the decimation rates and Striga height. On susceptible genotypes, the seeds yield losses due to Striga infestation varied from 28.4 to 36.6% while for the fodder yield these losses ranged between 23.63 to 41.03%. The analysis of F1 and F2 populations resulting from crossings between resistant varieties and the susceptible tester VYA demonstrated that resistance is was controlled by two factor inheritance with duplicate dominant genes (Rsg5a and Rsg5b for IT99K-573-1-1 and a single dominant gene (Rsg5 for IT98K-205-8. These two varieties provide newly additional sources of resistance to S. gesnerioides and can be used in programmes of genetic improvement of this legume.

  4. Effect of physical mutagen on expression of characters in arid legume pulse cowpea (Vigna unguiculata (L. Walp.

    Directory of Open Access Journals (Sweden)

    V.Ashok kumar, R.Usha kumari, N.Vairam and R.Amutha

    2010-07-01

    Full Text Available Studies on physical induced mutagenesis, gamma rays were performed by exposing the healthy and dry seeds of cowpeavariety CO 4 to gamma rays 20, 30, 40, 50, 60 and 70 kR. The study was to evolve economically important mutants withvaried seed coat colour as against dark grey coloured seed coat of CO 4. The LD50 value was found at 50kR for 60 Cogamma rays. Under field conditions, germination, seedling survival, plant height on 30th day, pollen fertility, seed fertility,pods per plant, pod length, seeds per pod, 100 seed weight and single plant yield was reduced as compared to the control. InM2 generation, viable macro mutants like dwarf mutant, spreading type, late mutant, semi sterile type, single and tricotyledonary leaf mutant, basal branching, multiple leaf mutant, white flower mutant, chimeric mutant and seed coat colourmutant were observed. Gamma rays induced higher proportion of chlorophyll mutations. Single type and multiple typemutations occurred more frequently. Economically important macro mutants such as brownish white seed coat colourmutants were observed in M2 generation.

  5. Mapping QTL for drought stress-induced premature senescence and maturity in cowpea [Vigna unguiculata (L.) Walp.].

    Science.gov (United States)

    Muchero, Wellington; Ehlers, Jeffrey D; Close, Timothy J; Roberts, Philip A

    2009-03-01

    Cowpea is an important crop for subsistence farmers in arid regions of Africa, Asia, and South America. Efforts to develop cultivars with improved productivity under drought conditions are constrained by lack of molecular markers associated with drought tolerance. Here, we report the mapping of 12 quantitative trait loci (QTL) associated with seedling drought tolerance and maturity in a cowpea recombinant inbred (RIL) population. One hundred and twenty-seven F(8) RILs developed from a cross between IT93K503-1 and CB46 were screened with 62 EcoR1 and Mse1 primer combinations to generate 306 amplified fragment length polymorphisms for use in genetic linkage mapping. The same population was phenotyped for maintenance of stem greenness (stg) and recovery dry weight (rdw) after drought stress in six greenhouse experiments. In field experiments conducted over 3 years, visual ratings and dry weights were used to phenotype drought stress-induced premature senescence in the RIL population. Kruskall-Wallis and multiple-QTL model mapping analysis were used to identify QTL associated with drought response phenotypes. Observed QTL were highly reproducible between stg and rdw under greenhouse conditions. Field studies confirmed all ten drought-response QTL observed under greenhouse conditions. Regions harboring drought-related QTL were observed on linkage groups 1, 2, 3, 5, 6, 7, 9, and 10 accounting for between 4.7 and 24.2% of the phenotypic variance (R(2)). Further, two QTL for maturity (R(2) = 14.4-28.9% and R(2) = 11.7-25.2%) mapped on linkage groups 7 and 8 separately from drought-related QTL. These results provide a platform for identification of genetic determinants of seedling drought tolerance in cowpea.

  6. Socio-economic aspects of the intensive growing of cowpeas (vigna unguiculata (L.) Walp.) in Kano, northern Nigeria

    OpenAIRE

    Van Damme, P.; W. Sampers; Pauwels, F.

    1986-01-01

    The costs and benefits of single crop cowpea and cowpea grown in, a traditional mixed cropping system are calculated for the Kano area, in northern Nigeria. If all the technical requirements are met, single crop cowpea is more profitable, on a financial return per unit land basis, than the traditional crop mixture. A one year effort to try and familiarize traditional farmers with a new variety and new production methods is clearly not enough and may even demotivate a number of farmers when th...

  7. El poder militar chino: el dragón alza el vuelo

    Directory of Open Access Journals (Sweden)

    Ignacio García Sánchez

    2016-01-01

    Full Text Available China es un actor geopolítico de talla universal. Sus dimensiones geopolíticas –físicas, económicas, humanas, sociopolíticas y militares– son abrumadoras. Pero, quizás, lo que impresiona y en cierta manera preocupa más es su impenetrabilidad. Referido al arte de la guerra se manifiesta de forma clara en utilizar la “decepción” como su principal estratagema –uno de sus principios básicos enunciados por Sun Tzu–. No se busca, por lo tanto, el enfrentamiento de las capacidades militares, sino la pugna entre las maestrías intelectuales. Así, el poder militar chino, tradicionalmente postergado por el poder imperial, no fue capaz de resistir el encuentro con el industrializado occidente que lo sojuzga durante el llamado “siglo infame de humillación”. Y no ha sido sino a través de dos de las más cruentas guerras de la historia de la humanidad y de la revolución comunista como el dragón herido ha vuelto a recuperar el control de su propio destino. A lomos de su Ejército Popular de Liberación, campesino e iletrado, vuelve a sus orígenes para consolidar esa única e imperecedera Civilización-Estado. Un ejército del pueblo y para el pueblo que, como brazo armado del Partido Comunista Chino, sólo mira hacia el interior de su gran muralla; conocedor del poder del resto de dimensiones geopolíticas; y que sólo empieza a alzar la vista y emprender el vuelo de la mano del milagro económico de las últimas décadas. Un poder militar orgulloso y capaz, que quiere protagonizar, junto con su sociedad, esta vez sí, “el sueño de China, el sueño del pueblo”, del “desarrollo pacífico”.

  8. Prohibir el mestizaje con chinos: solicitudes de amparo, Sonora, 1921-1935

    Directory of Open Access Journals (Sweden)

    Augustine-Adams, Kif

    2012-08-01

    Full Text Available In the state of Sonora, Mexico, at the beginning of the Twentieth Century, the Chinese population faced vicious discrimination including Law 31. Promulgated by the state legislature in late 1923, Law 31 prohibited marriage and other intimate relations between Chinese men and Mexican women. Through amparo petitions in federal court, up to and including the Supreme Court of Mexico, Chinese Mexican couples challenged the discrimination inherent in Law 31. Chinese Mexican amparo petitions against Law 31 demonstrate both the challenges and possibilities that law provided to the disenfranchised in Mexico at the beginning of the Twentieth Century.

    En el estado de Sonora la población china enfrentó una manifiesta discriminación expresada en la Ley 31 de 1923. Esta Ley prohibía el matrimonio y la mestización entre hombres chinos y mujeres mexicanas. Por medio de peticiones de amparo, parejas mexicano-chinas desafiaron esta legislación, llegando con los procesos legales hasta la Suprema Corte de Justicia. En los años 1924 y 1925, jueces federales en Sonora aplicaron los principios de la Constitución y de la legislación federal para proteger a la población china. Las peticiones de amparo demuestran tanto los desafíos como las posibilidades que el derecho otorgó a los residentes de México en las primeras décadas del siglo XX.

  9. Usos y acepciones de chino, china en el norte del Perú, siglos XVIII-XIX

    OpenAIRE

    Luis Andrade Ciudad; Fred Rohner

    2014-01-01

    ResumenSe estudian los usos de la palabra chino, china en el norte peruano, entre los siglos XVIII y XIX, a partir de documentos históricos y coplas de la lírica popular. En contra de propuestas anteriores, se confirma el uso efectivo del término, sobre todo en su forma femenina, y se postulan dos acepciones: la primera, vinculada a la terminología de castas, alude a un tipo de mestizaje que une los troncos indio y africano, con un componente menor, variable, de “sangre blanca”, mientras que ...

  10. Interspecific hybridization of Vigna radiata x 13 wild Vigna species for developing MYMV donar

    Directory of Open Access Journals (Sweden)

    M. Pandiyan , N.Senthil, N. Ramamoorthi, AR.Muthiah, N.Tomooka V.Duncan and T.Jayaraj

    2010-07-01

    Full Text Available Mungbean (Vigna radiata (L. Wilczek is having a desirable characters like short duration, high protein, less anti nutritionalfactors, nitrogen fixing capacity, suitable for inter cropping, making many kinds of foods for higher human consumption andcosmetics products and some of the undesirable characters like low yield, low test weight.The aim of the study is to checkcrossabil;ity of Vigna radiata with all wild Vigna species and to create variability through wide hybridization and to developsuperior segregants for yield component coupled with pest and disease resistance. The interspecific crosses were attemptedwith thirteen wild relatives of mungbean (V. radiata were employed with V. radiata as male parent. The highest pod set of25 and crossability of 21.92 per cent was recorded by the cross V. radiata x V. radiata var. sublobata and lowest pod set of2.0 per cent recorded by the cross V. radiata x V. dalzelliana in the direct cross combinations. The highest pollengermination 69.72 per cent was recorded by V. radiata x V. radiata var. sublobata. The estimates of pollen fertility wassufficient enough to recover F2 segregants in all the crosses combination.For all the traits in majority of the crosses, In the F2generation the skewness was positive indicating that predominance of dominant alleles. Among the direct crosses V. radiatax V. mungo var. silvestris exhibited highest value for four characters viz., number of clusters per branch, number of clustersper plant, number of pods per plant and single plant yield. The cross V. umbellata x V. radiata showed better performancefor the characters viz., number of branches and number of clusters in reciprocal direction In advanced generation of Vignaradiata x Vigna umbellata cross combination has expressed virus resistance for nine seasons. The same line was tested byagro inoculation for confirmation of resistance and was resulted found effective resistance. This line can be used MYMVdonar. Conclusion of

  11. Contraste pragmalingüístico de las expresiones de disculpa entre el español y el chino

    Directory of Open Access Journals (Sweden)

    Tzu Yu Liu

    2012-11-01

    Full Text Available Normal 0 21 false false false ES X-NONE X-NONE MicrosoftInternetExplorer4 Es bien sabido que nuestra forma de hablar y el uso del lenguaje están estrechamente relacionados con el factor sociocultural. El objetivo principal de este artículo es hacer una comparación de las fórmulas de disculpa más usadas en español y en chino, desde la perspectiva cultural y pragmática. Con ello esclarecemos la influencia sociocultural en la realización del acto de habla de disculpa en español peninsular y el chino de Taiwán. Analizamos las expresiones de disculpa desde el punto de vista de su contexto y de la cortesía, ya que estas fórmulas tienen diferentes funciones en ambos ámbitos. Además comparamos las semejanzas y diferencias de su uso para ilustrar los rasgos específicos de cada sociedad. De este modo, comprobamos que para comunicarnos con interlocutores de diferente cultura, siempre hay que tomar en consideración el factor sociocultural.  

  12. Unlicensed to Prescribe Herbs: A Chinese Healer - Médico Chino - in Puerto Rico, 1851-1853.

    Science.gov (United States)

    Rigau-Pérez, José G

    2016-06-01

    In Puerto Rico and Cuba, the phrase "can't be saved even by the Chinese physician" ("no lo salva ni el médico chino") indicates a person with an incurable disease. The documents at the Archivo General de Puerto Rico include three requests for a medical license from a Chinese immigrant, Juan de Dios Sian (Lin Hua Cheng). Despite lacking legal credentials, he used herbal therapies to treat chronically ill persons in Ponce, San Juan and Mayaguez from 1851 to 1853. Before arriving in Ponce he had spent four years in Cuba, where he is again found by 1865. Sian's petitions show that Puerto Rico, like Cuba, experienced a widely known "médico chino." The anecdote reminds us of important issues in our medical and social history: Asiatic immigration (earlier, larger and more diverse than usually considered), access to care (and its limitations), and the long history of herbal medicine in Oriental and Western cultures. Elements of this story, such as the eagerness for new treatments among patients who have derived no benefit from standard therapy, the ethics of medical licensing, the impotence of licensing agencies and the toleration of authorities regarding an unorthodox but popular healer, exemplify dilemmas that accompany medical practice at all times. PMID:27232873

  13. Distribution of Ground Motions for the 2008 Mw5.4 Chino Hills Earthquake

    Science.gov (United States)

    Yong, A.; Hough, S. E.; Wills, C. J.; Wald, D. J.

    2008-12-01

    The 29 July 2008 Mw5.4 Chino Hills earthquake was widely felt throughout the greater Los Angeles region. Over 40,000 people filled out the Community Internet Intensity Map (CIIM), also known as the "Did You Feel It?" questionnaire, and the ShakeMap for the event is constrained by amplitudes from over 500 stations. The CIIM responses provide Modified Mercalli Intensity (MMI) values for 802 Zip codes. Due to the dense population of the greater Los Angeles region, intensity values averaged within Zip codes provide good spatial correlation to the location of the actual reporting sites. We use a least-squares approach to fit the average MMI to a standard functional form, MMI = A - Br - C log r (where r represents distance from the observation site to the epicenter and A, B and C are constants), and calculate the residuals. The distribution of average intensities clusters closely around the curve of the equation, corroborating the conclusion (e.g., Atkinson and Wald, SRL, 2006) that CIIM intensities provide a consistent measure of earthquake ground motions. A comparison of CIIM intensities and instrumentally determined ShakeMap intensities reveals generally good correspondence, but suggests a tendency for ShakeMap intensities to be higher at basin sites. We conclude that the ShakeMap intensities reflect long-period amplification, whereas observed intensities were more controlled by higher frequency accelerations. Where both the CIIM map and ShakeMap show systematic variations, the distributions show a strong correlation with near-surface geological structure, as well as with basin depth. Our preliminary conclusions are: 1) as expected, intensities are lower at hard rock sites; 2) intensities are systematically higher in the deepest parts of the basin and significantly lower in shallow regions. Intensities are systematically lower to the west of the Newport-Inglewood fault correlating with subtle topographic relief and shallower basin depths; 3) there is a suggestion that

  14. La entonación de enunciados declarativos e interrogativos en chino mandarín hablado por taiwaneses

    Directory of Open Access Journals (Sweden)

    Wei-Li Kao

    2014-11-01

    Full Text Available El presente trabajo de investigación tiene el propósito de describir la entonación de los enunciados declarativos e interrogativos absolutos del chino mandarín hablado por taiwaneses. Teniendo en cuenta este objetivo, se ha elaborado un corpus de habla espontánea y se ha analizado siguiendo el método Análisis Melódico del Habla. El corpus está basado en 88 enunciados emitidos por 44 informantes, que provienen de las emisiones de dos programas de televisión taiwaneses. Los resultados obtenidos muestran que los enunciados declarativos e interrogativos absolutos tienen tendencia a finalizar con un contorno descendente leve, y además, se ha observado que el tono de los fonemas se modifica según la entonación del grupo fónico.

  15. Interspecific Hybridization AmongVigna Species%豇豆属食用豆类间的远缘杂交

    Institute of Scientific and Technical Information of China (English)

    刘长友; 范保杰; 曹志敏; 苏秋竹; 王彦; 张志肖; 程须珍; 田静

    2015-01-01

    粒的发育;黑吉豆与小豆、饭豆杂交时存在胚败育现象;饭豆作母本与小豆杂交经幼胚拯救可以获得可育的F1植株;小豆与近缘野生种的杂交亲和性优于饭豆;豇豆与其他食用豆类间杂交均未成功。%Objective]The aim of this study was to analyze the crossability of differentVigna species, which will facilitate the distant hybridization breeding ofVigna.[Method] Interspecific crossabilities were investigated among 5 cultivar species of Vigna,V. radiata (mungbean),V. mungo (black gram),V. umbellata (rice bean),V. anguluris (adzuki bean),V. unguiculata (cowpea), and 3 wild relative species of adzuki bean,V. minima,V. nakashiame andV. riukiuensis. Embryo rescue was conducted for 3 interspecific combinations, namely,V. radiata×V. angularis,V. angularis×V. umbellataandV. umbellata×V. angularis, respectively. The pod setting ratio of hybrid and performance of F1 plant were investigated to analyze the crossabilities between food legumes ofVigna genus.[Result] The genetic relationship between different food legumes and the choice of female parent influenced the result of distant hybridization. Except for cowpea cross combinations, hybrid pods were set successfully in the other interspecific crosses. The growth of hybrid pods and seeds showed variations in different combinations. No genetic disorders before fertilization were found from the hybridizations ofV. radiata×V. mungo,V. angularis×V. minima,V. angularis×V. nakashiame, andV. angularis×V. riukiuensis. The hybrid seeds of them germinated normally, and the hybrid plants were fertile, but the fruiting rates were lower. The pod setting ratio was significantly higher whenV. radiatawas selected as female parent in the interspecific crosses betweenV. radiataandV. umbellata. F1 plants were obtained without embryo culture, but they showed complete hybrid sterility. No fertile seeds were obtained even backcross toV. radiataorV. umbellata. Hybrid plants were obtained from cultured

  16. Vigna yadavii (Leguminosae: Papilionoideae, a new species from Western Ghats, India

    Directory of Open Access Journals (Sweden)

    Sayajirao Gaikwad

    2014-12-01

    Full Text Available A new species of Vigna Savi, subgenus Ceratotropis (Piper Verdc., Vigna yadavii S.P. Gaikwad, R.D. Gore, S.D. Randive & K.U. Garad, sp. nov. is described and illustrated here. It is morphologically close to Vigna dalzelliana (Kuntze Verdc. but differs in its underground obligate cleistogamous flowers on positively geotropic branches, hairy calyx, small corolla, linear style beak and dimorphic seeds with shiny seed coat.

  17. Application of a watershed model (HSPF) for evaluating sources and transport of pathogen indicators in the Chino Basin drainage area, San Bernardino County, California

    Science.gov (United States)

    Hevesi, Joseph A.; Flint, Lorraine E.; Church, Clinton D.; Mendez, Gregory O.

    2011-01-01

    A watershed model using Hydrologic Simulation Program-FORTRAN (HSPF) was developed for the urbanized Chino Basin in southern California to simulate the transport of pathogen indicator bacteria, evaluate the flow-component and land-use contributions to bacteria contamination and water-quality degradation throughout the basin, and develop a better understanding of the potential effects of climate and land-use change on water quality. The calibration of the model for indicator bacteria was supported by historical data collected before this study and by samples collected by the U.S. Geological Survey from targeted land-use areas during storms in water-year 2004. The model was successfully calibrated for streamflow at 5 gage locations representing the Chino Creek and Mill Creek drainages. Although representing pathogens as dissolved constituents limits the model's ability to simulate the transport of pathogen indicator bacteria, the bacteria concentrations measured over the period 1998-2004 were well represented by the simulated concentrations for most locations. Hourly concentrations were more difficult to predict because of high variability in measured bacteria concentrations. In general, model simulations indicated that the residential and commercial land uses were the dominant sources for most of the pathogen indicator bacteria during low streamflows. However, simulations indicated that land used for intensive livestock (dairies and feedlots) and mixed agriculture contributed the most bacteria during storms. The calibrated model was used to evaluate how various land use, air temperature, and precipitation scenarios would affect flow and transport of bacteria. Results indicated that snow pack formation and melt were sensitive to changes in air temperature in the northern, mountainous part of the Chino Basin, causing the timing and magnitude of streamflow to shift in the natural drainages and impact the urbanized areas of the central Chino Basin. The relation between

  18. El Centro de Arbitraje Chino-Europeo: una introducción al Reglamento de Arbitraje del CEAC de Hamburgo

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    Eckart Brödermann

    2014-06-01

    Full Text Available El Centro de Arbitraje Chino-Europeo (CEAC se especializa en casos de comercio e inversión provenientes de todas partes del mundo que guardan relación directa o indirecta con China, por remota que sea; el ceac está hecho a la medida de las necesidades del comercio con China. Este artículo ofrece, en primer lugar, un panorama general de los factores no jurídicos que se encuentran detrás del ceac, tales como el espíritu de mayor neutralidad y de cooperación. En segundo lugar, en su parte central, el escrito analiza las diferencias entre el Reglamento de Arbitraje del ceac de Hamburgo y el Reglamento de Arbitraje de la CNUDMI, sobre el cual está basado el primero. También se ofrece una descripción de la integración de otros instrumentos jurídicos (como la CISG y los Principios UNIDROIT sobre los contratos comerciales internacionales en la concepción jurídica del ceac, que fue discutida durante varios años contando, al final, con 470 colaboradores de 47 países.

  19. El Centro de Arbitraje Chino-Europeo: una introducción al Reglamento de Arbitraje del CEAC de Hamburgo

    Directory of Open Access Journals (Sweden)

    Eckard Brödermann

    2014-07-01

    Full Text Available El Centro de Arbitraje Chino-Europeo (CEAC se especializa en casos de comercio e inversión provenientes de todas partes del mundo que guardan relación directa o indirecta con China, por remota que sea; el ceac está hecho a la medida de las necesidades del comercio con China. Este artículo ofrece, en primer lugar, un panorama general de los factores no jurídicos que se encuentran detrás del ceac, tales como el espíritu de mayor neutralidad y de cooperación. En segundo lugar, en su parte central, el escrito analiza las diferencias entre el Reglamento de Arbitraje del ceac de Hamburgo y el Reglamento de Arbitraje de la CNUDMI, sobre el cual está basado el primero. También se ofrece una descripción de la integración de otros instrumentos jurídicos (como la CISG y los Principios UNIDROIT sobre los contratos comerciales internacionales en la concepción jurídica del ceac, que fue discutida durante varios años contando, al final, con 470 colaboradores de 47 países.

  20. CHROMIUM INDUCED CYTOTOXICITY IN BLACKGRAM (VIGNA MUNGO L.)

    OpenAIRE

    A. Chidambaram ، P. Sundaramoorthy ، A. Murugan ، K. Sankar Ganesh ، L. Baskaran

    2009-01-01

    Chromium is known to be highly toxic to biological systems. This study was designed to determine the mutagenic effects of different concentrations (0, 10, 25, 50, 100 and 200 mg/L) of hexavalent chromium on root tip cells of blackgram (Vigna mungo L. Hepper). The blackgram seeds were equi-spacially arranged in sterilized petriplates lined with filter paper and they were treated with different concentrations of chromium solution. In germination studies, the morphological growth parameters such...

  1. Effect of gamma radiation of 60Co in the variability of chinese bean [Vigna unguiculata (l.) Walpers] en R4M4

    International Nuclear Information System (INIS)

    Selection of plants of Chinese bean coming from seed irradiated with gammas of 60Co in the generation R4M4 (fourth recurrent irradiation, fourth segregate generation) were carried out, taking as selection approaches the plant architecture, the numbers of sheaths for plant, sheath longitude, position of the sheaths, grain size and resistance to plagues and illnesses. 17 lines were selected for grain and 3 lines with fodder characteristics of black grain color were also obtained. (Author)

  2. Genetic and physical mapping of candidate genes for resistance to Fusarium oxysporum f.sp. tracheiphilum race 3 in cowpea [Vigna unguiculata (L. Walp].

    Directory of Open Access Journals (Sweden)

    Marti Pottorff

    Full Text Available Fusarium oxysporum f.sp. tracheiphilum (Fot is a soil-borne fungal pathogen that causes vascular wilt disease in cowpea. Fot race 3 is one of the major pathogens affecting cowpea production in California. Identification of Fot race 3 resistance determinants will expedite delivery of improved cultivars by replacing time-consuming phenotypic screening with selection based on perfect markers, thereby generating successful cultivars in a shorter time period. Resistance to Fot race 3 was studied in the RIL population California Blackeye 27 (resistant x 24-125B-1 (susceptible. Biparental mapping identified a Fot race 3 resistance locus, Fot3-1, which spanned 3.56 cM on linkage group one of the CB27 x 24-125B-1 genetic map. A marker-trait association narrowed the resistance locus to a 1.2 cM region and identified SNP marker 1_1107 as co-segregating with Fot3-1 resistance. Macro and microsynteny was observed for the Fot3-1 locus region in Glycine max where six disease resistance genes were observed in the two syntenic regions of soybean chromosomes 9 and 15. Fot3-1 was identified on the cowpea physical map on BAC clone CH093L18, spanning approximately 208,868 bp on BAC contig250. The Fot3-1 locus was narrowed to 0.5 cM distance on the cowpea genetic map linkage group 6, flanked by SNP markers 1_0860 and 1_1107. BAC clone CH093L18 was sequenced and four cowpea sequences with similarity to leucine-rich repeat serine/threonine protein kinases were identified and are cowpea candidate genes for the Fot3-1 locus. This study has shown how readily candidate genes can be identified for simply inherited agronomic traits when appropriate genetic stocks and integrated genomic resources are available. High co-linearity between cowpea and soybean genomes illustrated that utilizing synteny can transfer knowledge from a reference legume to legumes with less complete genomic resources. Identification of Fot race 3 resistance genes will enable transfer into high yielding cowpea varieties using marker-assisted selection (MAS.

  3. Evaluation of Pre-Emergence Herbicides for Weed Control in Cowpea (Vigna unguiculata (L.) Walp.) in a Forest - Savanna Transition Zone

    OpenAIRE

    Sunday, Omovbude; Udensi, Udensi Ekea

    2013-01-01

    Aims: To evaluate the efficacy and economic benefits of selected pre-emergence herbicides for weed control in cowpea. Study Design: Randomized complete block design with four replications was used. Place and Duration of Study: Research and Teaching Farm of Ambrose Alli University in Ekpoma (Lat. 6º 45’N, Long. 6º 8’E), in a forest-savanna transition zone of Nigeria, between August and December 2005 and August and December 2006. Methodology: Five weed control treatments e...

  4. Effects of volatiles from Maruca vitrata larvae and caterpillar-infested flowers of their host plant Vigna unguiculata on the foraging behavior of the parasitoid Apanteles taragamae

    OpenAIRE

    Dannon, A.E.; Tamò, M.; Huis, van, A.; Dicke, M.

    2010-01-01

    The parasitoid wasp Apanteles taragamae is a promising candidate for the biological control of the legume pod borer Maruca vitrata, which recently has been introduced into Benin. The effects of volatiles from cowpea and peabush flowers and Maruca vitrata larvae on host selection behavior of the parasitoid Apanteles taragamae were investigated under laboratory conditions by using a Y-tube olfactometer. Naïve and oviposition-experienced female wasps were given a choice between several odor sour...

  5. Natural infection of cowpea (Vigna unguiculata (L.) Walp.) by toxigenic fungi and mycotoxin contamination in Benin, West Africa

    DEFF Research Database (Denmark)

    Houssou, P.A.; Ahohuendo, B.C.; Fandohan, P.;

    2009-01-01

    for the two periods. About 23 fungal species were identified on cowpea seed samples across zones of which Aspergillus flavus, a fungus that produces aflatoxins, was most frequently encountered. Fusarium species shown to produce fumonisins were not recorded from cowpea seeds. Overall incidence of A. flavus...... infection was found to increase after storage from 7.6% at T0 to 28.25% at T3. In spite of this natural infection of cowpea, very low levels of fumonisin and aflatoxin were detected. Only three out of the 92 cowpea samples, all collected at T0, were found to be fumonisin B1 positive with a mean level of 0.......03 mg/g. Similarly, only six samples out of the 92, all collected at T3, were aflatoxin B1 positive with mean levels of 3.58 µg/kg. Fumonisin (B2 and B3) and aflatoxin (B2, G1 and G2) were not detected in any of the samples. Contrary to the situation with maize and groundnut where high levels of toxin...

  6. Image based remote sensing method for modeling black-eyed beans (Vigna unguiculata) Leaf Area Index (LAI) and Crop Height (CH) over Cyprus

    Science.gov (United States)

    Papadavid, Giorgos; Fasoula, Dionysia; Hadjimitsis, Michael; Skevi Perdikou, P.; Hadjimitsis, Diofantos

    2013-03-01

    In this paper, Leaf Area Index (LAI) and Crop Height (CH) are modeled to the most known spectral vegetation index — NDVI — using remotely sensed data. This approach has advantages compared to the classic approaches based on a theoretical background. A GER-1500 field spectro-radiometer was used in this study in order to retrieve the necessary spectrum data for estimating a spectral vegetation index (NDVI), for establishing a semiempirical relationship between black-eyed beans' canopy factors and remotely sensed data. Such semi-empirical models can be used then for agricultural and environmental studies. A field campaign was undertaken with measurements of LAI and CH using the Sun-Scan canopy analyzer, acquired simultaneously with the spectroradiometric (GER1500) measurements between May and June of 2010. Field spectroscopy and remotely sensed imagery have been combined and used in order to retrieve and validate the results of this study. The results showed that there are strong statistical relationships between LAI or CH and NDVI which can be used for modeling crop canopy factors (LAI, CH) to remotely sensed data. The model for each case was verified by the factor of determination. Specifically, these models assist to avoid direct measurements of the LAI and CH for all the dates for which satellite images are available and support future users or future studies regarding crop canopy parameters.

  7. Effect of garlic extraction on injury by cowpea, Curculio Chalcodermes aenus Boheman (Coleoptera: Cucurlionidae), and other pests, to cowpea, Vigna unguiculata L. Walp

    Science.gov (United States)

    Garlic-based oils and extract formulations have been used as insecticides against various insects on numerous crops, but there are contradictions among findings on the insecticidal or repellent properties of garlic-based products. In a field plot test, the effects of garlic extract on control of th...

  8. Effect of Osmopriming Duration on Germination, Emergence, and Early Growth of Cowpea (Vigna unguiculata (L. Walp. in the Sudan Savanna of Nigeria

    Directory of Open Access Journals (Sweden)

    Ajit Singh

    2014-01-01

    Full Text Available Seed osmopriming could be a sustainable method to increase crop establishment, uniform emergence, and growth of plant on the field. Laboratory and field studies were carried out in 2010 cropping season at Usmanu Danfodiyo University, Sokoto, to study the effect of seed osmopriming duration on the germination, emergence, and growth of cowpea seeds. Treatments consisted of three osmopriming duration (soaking in 1% KNO3 salt for 6, 8, and 10 hrs, one hydroprimed control (10 hr, and an unprimed control. These five treatments were laid out in a completely randomized design (CRD replicated four times. The results showed that osmopriming with KNO3 for different durations was at par but was superior to unprimed treatments in terms of seed germination, emergence, plant height, and dry matter accumulation at 3 weeks after sowing. From this study, it can therefore be concluded that seeds of cowpea could be primed (both hydro and osmopriming for increased performance. However, osmopriming with KNO3 salt (soaked in 1% KNO3 salt solution and dried before sowing for 6 hours could result in greater seed germination and seedling height than hydropriming.

  9. Impact of the energy crop Jatropha curcas L. on the composition of rhizobial populations nodulating cowpea (Vigna unguiculata L.) and acacia (Acacia seyal L.).

    Science.gov (United States)

    Dieng, Amadou; Duponnois, Robin; Floury, Antoine; Laguerre, Gisèle; Ndoye, Ibrahima; Baudoin, Ezékiel

    2015-03-01

    Jatropha curcas, a Euphorbiaceae species that produces many toxicants, is increasingly planted as an agrofuel plant in Senegal. The purpose of this study was to determine whether soil priming induced by J. curcas monoculture could alter the rhizobial populations that nodulate cowpea and acacia, two locally widespread legumes. Soil samples were transferred into a greenhouse from three fields previously cultivated with Jatropha for 1, 2, and 15 years, and the two trap legumes were grown in them. Control soil samples were also taken from adjacent Jatropha-fallow plots. Both legumes tended to develop fewer but larger nodules when grown in Jatropha soils. Nearly all the nifH sequences amplified from nodule DNA were affiliated to the Bradyrhizobium genus. Only sequences from Acacia seyal nodules grown in the most recent Jatropha plantation were related to the Mesorhizobium genus, which was much a more conventional finding on A. seyal than the unexpected Bradyrhizobium genus. Apart from this particular case, only minor differences were found in the respective compositions of Jatropha soil versus control soil rhizobial populations. Lastly, the structure of these rhizobial populations was systematically imbalanced owing to the overwhelming dominance of a very small number of nifH genotypes, some of which were identical across soil types or even sites. Despite these weak and sparse effects on rhizobial diversity, future investigations should focus on the characterization of the nitrogen-fixing abilities of the predominant rhizobial strains. PMID:25466917

  10. H2O2 plays an important role in the lifestyle of Colletotrichum gloeosporioides during interaction with cowpea [Vigna unguiculata (L.) Walp].

    Science.gov (United States)

    Eloy, Ygor R G; Vasconcelos, Ilka M; Barreto, Ana L H; Freire-Filho, Francisco R; Oliveira, Jose T A

    2015-08-01

    Plant-fungus interactions usually generate H(2)O(2) in the infected plant tissue. H(2)O(2) has a direct antimicrobial effect and is involved in the cross-linking of cell walls, signaling, induction of gene expression, hypersensitive cell death and induced systemic acquired resistance. This has raised the hypothesis that H(2)O(2) manipulation by pharmacological compounds could alter the lifestyle of Colletotrichum gloeosporioides during interaction with the BR-3-Tracuateua cowpea genotype. The primary leaves of cowpea were excised, infiltrated with salicylic acid (SA), glucose oxidase + glucose (GO/G), catalase (CAT) or diphenyliodonium chloride (DPI), followed by spore inoculation on the adaxial leaf surface. SA or GO/G-treated plantlets showed increased H(2)O(2) accumulation and lipid peroxidation. The fungus used a subcuticular, intramural necrotrophic strategy, and developed secondary hyphae associated with the quick spread and rapid killing of host cells. However, CAT or DPI-treated leaves exhibited decreased H(2)O(2) concentration and lipid peroxidation and the fungus developed intracellular hemibiotrophic infection with vesicles, in addition to primary and secondary hyphal formation. These results suggest that H(2)O(2) plays an important role in the cowpea (C. gloeosporioides) pathosystem given that it affected fungal lifestyle during interaction. PMID:26228563

  11. Effects of volatiles from Maruca vitrata larvae and caterpillar-infested flowers of their host plant Vigna unguiculata on the foraging behavior of the parasitoid Apanteles taragamae

    NARCIS (Netherlands)

    Dannon, A.E.; Tamò, M.; Huis, van A.; Dicke, M.

    2010-01-01

    The parasitoid wasp Apanteles taragamae is a promising candidate for the biological control of the legume pod borer Maruca vitrata, which recently has been introduced into Benin. The effects of volatiles from cowpea and peabush flowers and Maruca vitrata larvae on host selection behavior of the para

  12. Effects of volatiles from Maruca vitrata larvae and caterpillar-infested flowers of their host plant Vigna unguiculata on the foraging behavior of the parasitoid Apanteles taragamae.

    Science.gov (United States)

    Dannon, Elie A; Tamò, Manuele; Van Huis, Arnold; Dicke, Marcel

    2010-10-01

    The parasitoid wasp Apanteles taragamae is a promising candidate for the biological control of the legume pod borer Maruca vitrata, which recently has been introduced into Benin. The effects of volatiles from cowpea and peabush flowers and Maruca vitrata larvae on host selection behavior of the parasitoid Apanteles taragamae were investigated under laboratory conditions by using a Y-tube olfactometer. Naïve and oviposition-experienced female wasps were given a choice between several odor sources that included (1) uninfested, (2) Maruca vitrata-infested, and (3) mechanically damaged cowpea flowers, as well as (4) stem portions of peabush plants carrying leaves and flowers, (5) healthy M. vitrata larvae, and moribund (6), and live (7) virus-infected M. vitrata larvae. Responses of naïve and oviposition-experienced female wasps did not differ for any of the odor source combinations. Wasps were significantly attracted to floral volatiles produced by cowpea flowers that had been infested with M. vitrata larvae and from which the larvae had been removed. Apanteles taragamae females also were attracted to Maruca vitrata-infested flowers after removal of both the larvae and their feces. Female wasps discriminated between volatiles from previously infested flowers and mechanically damaged flowers. Uninfested cowpea flowers attracted only oviposition-experienced wasps that had received a rewarding experience (i.e. the parasitization of two M. vitrata larvae feeding on cowpea flowers) before the olfactometer test. Wasps also were attracted to uninfested leaves and flowers of peabush. Moreover, they were also attracted to healthy and live virus-infected M. vitrata larvae, but not when the latter were moribund. Our data show that, similarly to what has been extensively been reported for foliar volatiles, flowers of plants also emit parasitoid-attracting volatiles in response to being infested with an herbivore.

  13. Intercropping Sorghum (Sorghum bicolor L.) and Cowpea (Vigna unguiculata L.): Effect of Bradyrhizobium Inoculation and Fertilization on Minerals Composition of Sorghum Seeds

    OpenAIRE

    Ekhlas M. Musa; Elsiddig A. E. Elsheikh; Isam A. Mohamed Ahmed; Babiker, Elfadil E.

    2012-01-01

    This investigation was carried out in order to gain a better understanding of the effect of Bradyrhizobium inoculation and fertilization on the nutritional values of macro- and micronutrients of sorghum intercropped with cowpea in the field under rainfed conditions. The results of the present study demonstrated that intercropping, Bradyrhizobium inoculation, and N and P fertilization had significant effect on the mineral status of sorghum seeds. Intercropping significantly ( ≤ 0 . 0 5 ) inc...

  14. Effet positif de la fertilisation phosphatée sur les composantes du rendement du niébé (Vigna unguiculata L. en Tunisie

    Directory of Open Access Journals (Sweden)

    Ben Naceur, M.

    1991-01-01

    Full Text Available Positive effect of phosphate fertilization on component's yield of cowpea in Tunisia. Among the small lakes in the flooded area of the river Akagera, in eastern Rwanda, lake Ihema (area : 90 km2, mean depth : 4 m is the most important. The density of phytoplankton in the water is high but dissolved salts concentration is low. Ichtyofauna of the lake consists of 34 species which were occasionally exploited by tanzanian fishermen. That is why, since 1980, the government of Rwanda with the aid of the Belgian Cooperation Agency for Development have initiated an official fishery. Total yield, fishing effort and financial investments are analysed over a 7 years period (1981 to 1987 in order to precise the socio-economical efficiency of this cooperation project. Total fishery effort and total yield have increased regularly until 1983, reaching 186 tons with a fishing effort of 9.231 outings. Since that year, the production has stabilized at a level of 156-199 t/year with only two taxa exploited : Tilapia spp and Clarias gariepinus. The analysis of book-keeping and annual accountant balance of the Ihema fishery shows that the annual exploitation ratio fluctuates between 81 and 142, 5 % ; this indicates a difficult control of the expenditures at least during some years (1984, 1985 and 1986. The profit ratio shows a positive return (± 10 % since 1981 to 1983, then a depression during 1984 and a continual increase during the next years to become positive again in 1987. Continuous evaluations of these simple parameters allowed to take some actions in order to maintain the efficiency of the fishery. This new activity will be maintained if there is a good fish stock management and rigorous accounts.

  15. Effects of irradiation on the cowpea weevil (Callosobruchus maculates F. and moisture sorption isotherm of cowpea seed (Vigna unguiculata L. Walp

    Directory of Open Access Journals (Sweden)

    B. Darfour

    2012-03-01

    Full Text Available Cowpeas during storage may be attacked by a number of biological agents (microorganisms, rodents, and insects which results in losses in the quality and quantity of the stored seeds. One of the means of reducing these losses is through the application of radiation processing. The aim of this study was to determine the effects of gamma irradiation on a major storage insect pest, Callosobruchus maculatus F. and on moisture sorption isotherms of cowpea seeds in storage. The cowpeas were infested with adults C. maculatus and then irradiated at doses of 0.0, 0.25, 0.5, 0.75, 1.0 and 1.5 (kGy at a dose rate of 1.074 kGyhr-1. Samples were stored for 1 month under controlled temperature (27.3-30 oC and humidity (70-85 % during which counting of the insects was done every 48 hours and those alive or dead noted. Moisture sorption isotherms of the cowpea samples were equally determined by establishing equilibrium relative humidity (ERH of 55, 65, 75, 85 and 95 % using a formulation of glycerol-water mixture at temperature of 30+-1 and the weight (loss or gain of the samples was determined every 2 days. Irradiation at a dose of 0.25 kGy killed the C. maculatus within eight days and therefore 0.25 kGy would be economically beneficial as a control dose. There was significant difference (p is less than 0.05 in the percent mortality between the irradiated and the non-irradiated weevils, and the percent mortality increased with increase in the radiation dose. At moisture content of 14 % the irradiated cowpea samples were safely stored for one month at an equilibrium relative humidity of 60-75 % at a temperature of 29+-2 oC.

  16. STRAIN DIFFERENCES IN TWO SPECIES OF CALLOSOBRUCHUS (COLEOPTERA: BRUCHIDAE DEVELOPING ON SEEDS OF COWPEA {VIGNA UNGUICULATA (L.} AND GREEN GRAM {V. RADIATA (L.}

    Directory of Open Access Journals (Sweden)

    PHIL DOBIE

    1991-01-01

    Full Text Available Ovipositional behaviour, development period, and density effect on adult survival of C. maculatus strains from Indonesia, Nigeria, and Yemen, and C. chinensis strains from Indonesia and Kenya on cowpea and green gram were studied at 20°C and 70% relative humidity. Variations on ovipositional behaviour were found among C. maculatus as well as among C. chinensis strains. Variations on developmental period were found only among C. maculatus strains. The developmental period of Callosobruchus spp. was shorter on green gram than that on cowpea. Density effect was remarkably found only on adult survival of C. maculatus Yemen strain. These results make useful contribution to the species biology, and have important implication if strains of these species are accidentally imported to countries, or when new legume crops are introduced. INTRODUCTION Beetles belonging to the family Bruchidae are the most important insect pests of stored legumes. Infestation by bruchids causes losses of weight, nutritional value and germination potential, and therefore the commercial value of the commodity may be reduced (Southgate 1978; Dick and Credland 1986. The most economically important and widespread bruchids species are the cowpea seed beetle, Callosobruchus maculatus (Fabricius, and the Adzuki bean beetle, C. chinensis (Linnaeus (Southgate 1978; TDRI 1984. The use of resistant varieties of cultivated legumes is one of the recommended control methods of bruchid infestations. Varietal resistance against Callosobruchus has been reported in cowpeas and chickpea (Dobie 1981; Raina 1971; Singh 1978.

  17. Evaluation of native and collected Germplasm for earliness Seed traits and resistance to rust, CMV and leaf spot in Cowpea [Vigna unguiculata (L. Walp].

    Directory of Open Access Journals (Sweden)

    K Sanjeev. Deshpand, B.R.Patil, P. M. Salimath , J.M. Nidagundi and S. Karthigeyan

    2010-07-01

    Full Text Available In the present study, 225 germplasm collections of cowpea including local types were evaluated in augmented design alongwith C152 (check for high test weight, desirable seed and pod features, earliness and resistance to Cowpea rust (Uromycesvignae, Cowpea Mosaic Virus (CMV and Cercospora leaf spot for two years ( kharif 2008 and kharif 2009 at Dharwad. Theresults of the investigation revealed that fifteen of the accessions showed bold seeds (test weight > 10g. Accession numberIC202932 and IC247435 showed extra bold seeds (test weight >14g and also bold pods. About 15 germplasm accessions werefound to be highly resistant to rust; 10 accessions displayed HR reaction to CMV and about 5 accessions showed highlyresistant reaction against leafspot. With respect to Multiple Disease Resistance, accession numbers IC201095, IC257406,IC257435 showed highly resistant reaction against leaf spot and CMV and accessions IC257410 and IC214753 showedresistance against rust and leaf spot. Some of the accessions identified against earliness are IC201087, IC201099,IC202707and IC202709 (68-75 days maturity. Such of the superior germplasm lines identified for earliness, multiple diseaseresistance, bold seed (100 seed weight > 12g and desirable seed features would be further useful in Cowpea breeding programas parents.

  18. EFFECT OF CRUDE CASSAVA WATER EXTRACT AS A NATURAL HERBICIDE ON PROXIMATE COMPOSITION AND BIOACCUMULATION OF HYDROCYANIC ACID IN FOOD COMPONENTS OF COWPEA -VIGNA UNGUICULATA (L WALP

    Directory of Open Access Journals (Sweden)

    Olajumoke Oke FAYINMINNU

    2013-06-01

    Full Text Available This study was a field trial of two experiments to examine the effect of crude cassava water extract (CCWE as a natural post-emergence herbicide on nutritional quality and bioaccumulation of hydrocyanic acid in cowpea seeds. The spraying of CCWE on cowpea plants was carried out weekly for 5weeks. Treatments of CCWE at 25 and 50% concentrations of MS6 (Manihot Selection, TMS30555 (Tropical Manihot Selection and Bulk CCWE (different cassava varieties, hand weeded and unweeded (controls were laid in randomised complete block design with three replications respectively. At maturity, dry samples of cowpea `Ife brown` seeds were ground to fine powder and the proximate composition and bioaccumulation of hydrocyanic acid in the two experiments were determined. Significant variations (p0.05 among the herbicide treatments. It was therefore recommended that CCWE could be used as a natural post-emergence herbicide in cowpea production without altering the nutritional quality and residue of hydrocyanic acid in cowpea seeds.

  19. Performance of cowpea (Vigna unguiculata) and pearl millet (Pennisetum glaucum) intercropped under Parkia biglobosa in an agroforestry system in Burkina Faso

    DEFF Research Database (Denmark)

    Osman, Ahmed Nur; Ræbild, Anders; Christiansen, Jørgen Lindskrog;

    2011-01-01

    the shade of six randomly selected Parkia biglobosa trees during one season in south-central Burkina Faso. The area under the canopy of each tree was divided into four main plots according to cardinal directions, and sub-divided into three concentric zones. Control plots were established outside the tree...

  20. Application of organic compound produced from uçá crab Ucides cordatus cordatus for the cultivation of caupi beans Vigna unguiculata (L. Walp

    Directory of Open Access Journals (Sweden)

    Francisco das Chagas Neto

    2009-12-01

    Full Text Available The study goal is to propose an alternative to reduce crab’s residues generated by beach tents of the city of Fortaleza, Ceará State, transforming it into an organic compound to apply it in the cultivation of beans caupi. We tried to analyze the waste’s composition produced in those restaurants, as its chemical composition, yield and productivity response to culture. For this research were tested twenty-two treatments with four replications. The percentage of organic matter found in recyclable waste was approximately 87.34%, of which 52% is a waste of crab. The average yield in the production of fertilizer is approximately 24%. The results revealed the great potential of this compound for use in the agriculture. As the parameters of nitrogen, phosphorus, calcium and magnesium it gained prominence because their percentages found in organic products, which ranged from two to four times more compared with a sample of cattle manure..

  1. Saberes y experiencias de vida de una joven, hija de padres inmigrantes chinos y su familia en Andalucía. Un estudio biográfico

    OpenAIRE

    Mancila Mancila, Iulia

    2015-01-01

    Esta tesis doctoral se centra en la reconstrucción de la biografía de una joven de origen chino y su familia en España con el objetivo de explorar las experiencias vividas, las luchas, las dificultades, los retos, los valores y las subjetividades en varios contextos sociales, locales y trasnacionales: escuela, familia, comunidad y sociedad en general. En particular, para dar respuesta a las preguntas de investigación he optado por la perspectiva biográfica como la más idónea para describir la...

  2. Aspectos de la traducción en chino moderno. Un breve estudio sobre Ba Jin y su traducción de Castelao

    OpenAIRE

    Chen, Da

    2015-01-01

    La traducción de textos originalmente escritos en español llevada a cabo por escritores chinos del siglo XX nos ofrece una oportunidad para explorar algunos aspectos de la teoría de la traducción en la China contemporánea y nos permite acercarnos a la noción de la interculturalidad en la era de la globalización. Este trabajo analiza la traducción de breves fragmentos de textos de Castelao (1886-1950) realizada por el célebre escritor Ba Jin 巴金 (1904-2005). El objetivo principal es analizar la...

  3. Wangwen shengyi o traducir al pie de la letra catálogos de empresas del español al chino

    OpenAIRE

    Lu, Yan; Rovira-Esteva, Sara

    2009-01-01

    La globalización acelera cada vez más los contactos comerciales entre las empresas chinas, españolas e iberoamericanas. La traducción de los documentos de presentación de una empresa y de sus productos es muy importante para que las dos partes se conozcan mutuamente, establezcan buenas relaciones y hasta lleguen a ser socios estables. Sin embargo, a causa de las diferencias estructurales y culturales entre el español y el chino, así como por las limitaciones del propio traductor, muchas veces...

  4. EFICIENCIA AGRONÓMICA DE NITRÓGENO Y FÓSFORO EN LA PRODUCCIÓN DE FRIJOL CHINO EN ESPALDERA DE MAÍZ

    OpenAIRE

    Patricio Apáez Barrios; José Alberto Salvador Escalante Estrada; Víctor Manuel Olalde Gutiérrez; Porfirio Ramírez Vallejo; Eliseo Sosa Montes; Stephen Douglas Koch Olt

    2013-01-01

    El frijol chino (FCH), por su alto contenido de proteínas, carbohidratos, grasas y fibra, es fundamental en la alimentación humana. Se han obser vado incrementos en la rentabilidad económica al cultivarse en espaldera de maíz. Sin embargo, en este sistema, el requerimiento nutrimental, principalmente de nitrógeno (N) y fósforo (P) podría ser mayor. Los objetivos de esta investigación fueron: a) determinar en FCH en espaldera de maíz, el efecto de la aplicación de N y P sobre la biomasa total,...

  5. Usos y acepciones de chino, china en el norte del Perú, siglos XVIII-XIX

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    Luis Andrade Ciudad

    2014-07-01

    Full Text Available ResumenSe estudian los usos de la palabra chino, china en el norte peruano, entre los siglos XVIII y XIX, a partir de documentos históricos y coplas de la lírica popular. En contra de propuestas anteriores, se confirma el uso efectivo del término, sobre todo en su forma femenina, y se postulan dos acepciones: la primera, vinculada a la terminología de castas, alude a un tipo de mestizaje que une los troncos indio y africano, con un componente menor, variable, de “sangre blanca”, mientras que la segunda hace referencia a una mujer joven, generalmente campesina. Se estudia también el uso de la palabra como una fórmula de apelación a la amada en la lírica popular y se concluye que, aunque probablemente esta fórmula se derivó de la terminología racial, la palabra rápidamente adquirió matices afectivos positivos, con lo cual permitió aludir a la mujer cercana, aquella que era posible cortejar. Se sugiere que la antigua acepción ‘criada’, central en las fuentes coloniales del XVI y el XVII, se empezó a desdibujar en el período estudiado y, en línea con trabajos previos, se postula una competencia entre este lexema y la palabra homófona referida a las migrantes asiáticas desde fines del XIX.Palabras clave: categorización racial de seres humanos, lexicología, norte del Perú, siglos XVIII y XIXAbstractThis paper studies the uses of the word chino, china in northern Peru in the eighteenth and nineteenth centuries, via historical documents and popular poetry couplets. Contrary to previous proposals, we corroborate the actual use of this term, especially in its feminine form, and we propose two possible meanings for it: the first, linked to caste terminology, refers to a type of “racial mix” between “Indian” and “African”, with a minor and variable component of “white blood”, while the second refers to a young woman, usually a peasant. We also examine the use of the word as a formulaic

  6. Comparative Proximate and Elemental Analysis of Four Species of Cowpea Beans Seed Coat (Vigna Ungucuilata in Enugu State Nigeria

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    Ape David I

    2015-10-01

    Full Text Available Cowpea is an important bean which play significant role in the diets of Africans. It serves as a major source of protein in the absence of sufficient animal protein for the population. Four varieties large white beans seed coat (LWB small white beans seed coat (SWB large brown beans seed coat (LBB and small brown beans seed coat (SBB of cowpeas (Vigna unguiculata were analyzed for their proximate and elemental contents. These varieties belong to the same species in the family leguminosae. The brown and white seed coats were found to be nutritious. Both contained carbohydrate, protein, fibres and minerals such as calcium, magnesium, manganese and phosphorus. The moisture content was found to be 9.70 and 14.0% for (LWB and (SWB, and 11.20 and 10.80% for (LBB and (SBB respectively with the (SWB having the higher amount. The carbohydrate content analyzed was found to be 9.0 and 10.81% for (LWB and (SWB, and 8.40 and 7.80% for (LBB and (SBB with the (SWB having the higher value. The oil content was 0.37 and 0.21% for (LWB AND (SWB and 0.41 and 0.29% for (LBB and (SBB. The crude fibre was 70.55 and 68.20% (LWB AND (SWB and 68.17 and 70.01% for (LBB and (SBB respectively. The protein was 2.28 and 1.98% for (LWB AND (SWB and 2.52 and 2.40% for (LBB and (SBB respectively. The ash content was 8.1 and 4.8% for (LWB AND (SWB and 9.3 and 8.7% for (LBB and (SBB. The elemental analysis in the four species gave the following ranges:- Mg from 0.72 – 0.49ppm with (SWB having highest concentration and (LBB with lowest, Ca from 3.10 – 2.29ppm with (LBB highest and (LWB lowest, Mn from 0.31 – 0.18ppm with (LWB highest concentration and (SBB having the least, and finally P from 0.03 – 0.01ppm with (LBB with highest concentration and (SWB least concentration. From the analyses carried out it shows that cowpea beans seed coats are a good nutritional food source with higher crude fibre content.

  7. Agrobacterium tumefaciens-mediated transformation of Vigna mungo (L.) Hepper.

    Science.gov (United States)

    Karthikeyan, A S; Sarma, K S; Veluthambi, K

    1996-01-01

    Transformed Vigna mungo (blackgram) calli were obtained by cocultivating segments of primary leaves with Agrobacterium tumefaciens vir helper strains harbouring the binary vector pGA472 having kanamycin resistance gene as plant transformation marker. Transformed calli were selected on Murashige and Skoog medium supplemented with 50 mg/l kanamycin and 500 mg/l carbenicillin. Transformed calli were found to be resistant to kanamycin up to 900 mg/l concentration. Expression of kanamycin resistance gene in transformed calli was demonstrated by neomycin phosphotransferase assay. Stable integration of transferred DNA into V. mungo genome was confirmed by Southern blot analysis.

  8. Isolation and structural analysis of ajugose from Vigna mungo L.

    Science.gov (United States)

    Kotiguda, Girigowda; Peterbauer, Thomas; Mulimani, Veerappa H

    2006-09-01

    The hexasaccharide ajugose, alpha-D-galactopyranosyl-(1-->6)-alpha-D-galactopyranosyl-(1-->6)-O-alpha-D-galactopyranosyl-(1-->6)-alpha-D-galactopyranosyl-(1-->6)-alpha-D-glucopyranosyl-(12)-beta-D-fructofuranoside, generally uncommon in legumes, was detected in the seeds of Vigna mungo L. by TLC and paper chromatography. Ajugose was then isolated by silica gel chromatography and its structure was established by acid and enzymatic hydrolysis, fast atom bombardment mass spectrometry and both one- and two-dimensional 1H and 13C NMR techniques.

  9. Infectivity analysis of two variable DNA B components of Mungbean yellow mosaic virus-Vigna in Vigna mungo and Vigna radiata

    Indian Academy of Sciences (India)

    V Balaji; R Vanitharani; A S Karthikeyan; S Anbalagan; K Veluthambi

    2004-09-01

    Mungbean yellow mosaic virus-Vigna (MYMV-Vig), a Begomovirus that causes yellow mosaic disease, was cloned from field-infected blackgram (Vigna mungo). One DNA A clone (KA30) and five different DNA B clones (KA21, KA22, KA27, KA28 and KA34) were obtained. The sequence identity in the 150-nt common region (CR) between DNA A and DNA B was highest (95%) for KA22 DNA B and lowest (85.6%) for KA27 DNA B. The Rep-binding domain had three complete 11-nt (5′-TGTATCGGTGT-3′) iterons in KA22 DNA B (and KA21, KA28 and KA34), while the first iteron in KA27 DNA B (5′-ATCGGTGT-3′) had a 3-nt deletion. KA27 DNA B, which exhibited 93.9% CR sequence identity to the mungbean-infecting MYMV, also shared the 3-nt deletion in the first iteron besides having an 18-nt insertion between the third iteron and the conserved nonanucleotide. MYMV was found to be closely related to KA27 DNA B in amino acid sequence identity of BV1 (94.1%) and BC1 (97.6%) proteins and in the organization of nuclear localization signal (NLS), nuclear export signal (NES) and phosphorylation sites. Agroinoculation of blackgram (V. mungo) and mungbean (V. radiata) with partial dimers of KA27 and KA22 DNA Bs along with DNA A caused distinctly different symptoms. KA22 DNA B caused more intense yellow mosaic symptoms with high viral DNA titre in blackgram. In contrast, KA27 DNA B caused more intense yellow mosaic symptoms with high viral DNA titre in mungbean. Thus, DNA B of MYMV-Vig is an important determinant of host-range between V. mungo and V. radiata.

  10. Infectivity analysis of two variable DNA B components of Mungbean yellow mosaic virus-Vigna in Vigna mungo and Vigna radiata.

    Science.gov (United States)

    Balaji, V; Vanitharani, R; Karthikeyan, A S; Anbalagan, S; Veluthambi, K

    2004-09-01

    Mungbean yellow mosaic virus-Vigna (MYMV-Vig), a Begomovirus that causes yellow mosaic disease, was cloned from field-infected blackgram (Vigna mungo). One DNA A clone (KA30) and five different DNA B clones (KA21, KA22, KA27, KA28 and KA34) were obtained. The sequence identity in the 150-nt common region (CR) between DNA A and DNA B was highest (95%) for KA22 DNA B and lowest (85.6%) for KA27 DNA B. The Rep-binding domain had three complete 11-nt (5'-TGTATCGGTGT-3') iterons in KA22 DNA B (and KA21, KA28 and KA34), while the first iteron in KA27 DNA B (5'-ATCGGTGT-3') had a 3-nt deletion. KA27 DNA B, which exhibited 93.9% CR sequence identity to the mungbean-infecting MYMV, also shared the 3-nt deletion in the first iteron besides having an 18-nt insertion between the third iteron and the conserved nonanucleotide. MYMV was found to be closely related to KA27 DNA B in amino acid sequence identity of BV1 (94.1%) and BC1 (97.6%) proteins and in the organization of nuclear localization signal (NLS), nuclear export signal (NES) and phosphorylation sites. Agroinoculation of blackgram (V. mungo) and mungbean (V. radiata) with partial dimers of KA27 and KA22 DNA Bs along with DNA A caused distinctly different symptoms. KA22 DNA B caused more intense yellow mosaic symptoms with high viral DNA titre in blackgram. In contrast, KA27 DNA B caused more intense yellow mosaic symptoms with high viral DNA titre in mungbean. Thus, DNA B of MYMVVig is an important determinant of host-range between V. mungo and V. radiata.

  11. Allelopathic effect of Solanum melongena L. on Vigna radiata L.

    Directory of Open Access Journals (Sweden)

    Deepti Singh

    2014-08-01

    Full Text Available The present study has been carried out to investigate the allelopathic effect of aqueous leaf leachate of Solanum melongena L. on Vigna radiata L. The effects of leachate on germination, radicle length, plumule length, protein content and cell division in root tip meristems of seedlings of Vigna were studied. The seeds of mungbean were soaked with leaf leachate of 10, 25, 50, 75 and 100% concentrations for 4h. Bioassay indicated that there was dose-dependent inhibition of germination and seedling growth. Protein content was found to be reduced by the leachate of different concentrations as compared with control. The study also revealed that antioxidative enzymes, viz. superoxide dismutase, catalase and peroxidase activities increased with the increase in concentration of aqueous leaf leachate. Mitotic activity in root-tip cells of mungbean was found to be reduced and the impact was dose-dependent. However, chromosomal abnormalities, viz. fragment, precocious separation, sticky chromosome, disturbed metaphase and bridge were found to be increased with increasing concentrations of leachate.

  12. Isolation & Characterization of Rhizobia and their Effect on Vigna radiata Plant

    OpenAIRE

    Prerna Rajpoot and Kain Singh Panwar

    2013-01-01

    Rhizobia is Gram negative bacteria that fix nitrogen , bacteria colonize plant cell with root nodules and commonly found in pulse . In present study rhizobia isolated from root nodules of vigna radiata and characterized morphologically, biochemical test were to as certain its physiology under normal conditions , three bacterial strain (Rp1 , Rp2, Rp3) were tested for their effect on root, Shoot and no. of nodules of vigna radiata plant in green house condition. Comparatively in all th...

  13. Vigna radiata as a New Source for Biotransformation of Hydroquinone to Arbutin

    OpenAIRE

    Zahra Tofighi, Mohsen Amini, Mahzad Shirzadi, Hamideh Mirhabibi, Negar Ghazi Saeedi, Narguess Yassa

    2016-01-01

    Background: The suspension culture of Vigna radiata was selected for biotransformation of hydroquinone to its β-D-glucoside form (arbutin) as an important therapeutic and cosmetic compound. Methods: The biotransformation efficiency of a Vigna radiata cell culture in addition to different concentrations of hydroquinone (6-20 mg/100 ml) was investigated after 24 hours in comparison to an Echinacea purpurea cell culture and attempts were made to increase the efficacy of the process by adding eli...

  14. Determinação da difusividade e da energia de ativação para feijão macassar (Vigna unguiculata (L. Walp., variedade sempre-verde, com base no comportamento da secagem Determination of diffusivity and activation energy for cowpea grains (Vigna unguiculata (L. Walp., alwaysgreen variety, based on its drying behavior

    Directory of Open Access Journals (Sweden)

    Wilton P. da Silva

    2008-06-01

    Full Text Available Neste artigo, teve-se o objetivo de determinar curvas de secagem em camada fina para grãos de feijão macassar, variedade sempre-verde. Visou-se, também, à determinação da difusividade efetiva da água no interior do produto, bem como a energia de ativação. Para tal, foram utilizadas amostras com 150 g de feijão com teor inicial de água de 37% (bu, nas temperaturas de 40; 50 e 60 ºC. A velocidade do ar do secador convectivo foi mantida em 1,0 m s-1 e, durante todo o processo, os valores médios da temperatura e da umidade relativa do ar ambiente foram, respectivamente, 29 ºC e 58%. A difusividade efetiva da água no produto foi determinada, em cada temperatura, por meio da lei de Fick, pressupondo um modelo esférico para os grãos. Uma expressão para a difusividade efetiva da água em função da temperatura foi determinada por meio do ajuste da equação de Arrhenius aos dados obtidos. A análise dos resultados possibilita concluir que a equação de Page representa, de forma satisfatória, o processo de secagem em todas as condições estudadas. O modelo esférico estabelecido para os grãos é satisfatório, resultando em difusividade efetiva que variou de 7,13x10-11 até 14,0x10-11 m²s-1, e em energia de ativação igual a 26,9 kJ mol-1. Foi observado que, para a temperatura do ar de secagem mantida em 60 ºC, houve a formação de fissuras em quantidade significativa de grãos.In this paper it was determined the thin layer drying curves for cowpea grains, always-green variety. It was also determined the effective water diffusivity inside the product and its activation energy. For these purposes, 150 g samples of cowpea grains dried at temperatures of 40; 50 and 60 ºC were used. The drying air velocity was maintained constantly at 1.0 m s-1 during the drying process. The drying air temperature and relative humidity were 29 ºC and 58%, respectively. The effective water diffusivity inside the product was determined, for each drying temperature, by using Fick’s equation assuming a spherical shape for the grains. A mathematical expression for the effective water diffusivity inside the product as a function of temperature was determined by fitting Arrhenius’s equation to the observed data. According to the analysis of the results, it is possible to conclude that the Page equation can be used to satisfactorily represent the cowpea grains drying process for the conditions of this research. The assumption of a spherical shape for the grains was satisfactory, resulting in a water effective diffusivity varying from 7.13x10-11 to 14.00x10-11 m²s-1 and activation energy equal to 26.9 kJ mol-1. It was also observed that for a drying air temperature of 60 ºC a significant amount of grains presented fissures.

  15. Modificação da concentração de amido resistente em feijão macassar (Vigna unguiculata L. Walp por tratamento hidrotérmico e congelamento Modification of the concentration of resistant starch in macassar bean (Vigna unguiculata L. Walp hydrothermal process and freezing

    Directory of Open Access Journals (Sweden)

    Silvana Magalhães Salgado

    2005-06-01

    Full Text Available Considerando a importância das propriedades funcionais do amido resistente para a saúde, o elevado consumo de feijão macassar no Nordeste brasileiro e os efeitos do processo térmico e da estocagem sobre a estrutura do amido, foi realizado este trabalho com o objetivo de avaliar a influência de diferentes tratamentos hidrotérmicos (cocção sob pressão e sem pressão e do armazenamento a 20ºC por 15 e 30 dias, sobre a formação de amido resistente do feijão macassar em dois estádios de maturação (verde e seco. Para analisar os fatores combinados foi realizado um planejamento fatorial do tipo 2³ com três variáveis independentes (fatores e uma variável dependente (resposta. Todas as variáveis exerceram efeitos significativos na produção de AR e a maior formação foi registrada para o feijão seco devido à hidratação prévia a cocção sem pressão, além do tempo de armazenamento congelado de 30 dias. No que diz respeito às analises físicas, após os tratamentos hidrotérmicos, ocorreram transformações nos padrões de cristalinidade e no aspecto morfológico dos feijões.Considering the importance of the functional properties of resistant starch for health, the high consumption of the macassar bean in the northeast of Brazil and the effects of the thermal process and storage on the structure of the starch, this work was implemented to evaluate the influence of different hydrothermal treatments (cooking under pressure and whithout pressure and of storage at 20ºC for 15 and 30 days, on the formation of resistant starch in the macassar bean, in two stages of maturation (green and dryed. To analyse the combined factors, a factorial design type 2³ was realized with three independent variables (factors and one dependent variable (response. All the variables exercised significant effects on the production of the resistant starch and the highest formation was registered for the dried bean due to the previous hydration before cooking without pressure, as well as the 30 day frozen storage. Concerning the physics analysis after the hydrothermic treatments there are transformations in the cristalinity patterns and in the morphologic aspect of the beans.

  16. O estresse salino retarda o desenvolvimento morfofisiológico e a ativação de galactosidases de parede celular em caules de Vigna unguiculata Salt stress delays morphophysiological development and activation of cell wall galactosidases in Vigna unguiculata stems

    Directory of Open Access Journals (Sweden)

    Fabrício Bonfim Sudério

    2011-03-01

    Full Text Available Com o objetivo de examinar o envolvimento das α- e β-galactosidases na expansão celular de caules de plântulas de feijão-de-corda submetidas a estresse salino durante o estabelecimento da plântula, e de analisar os efeitos do estresse salino no desenvolvimento das plântulas e nas atividades enzimáticas, sementes de feijão-de-corda Pitiúba foram semeadas em água destilada e em solução de NaCl 100 mM. Foram coletados caules em diferentes estádios de desenvolvimento e com diferentes tempos após a semeadura. Avaliou-se o crescimento através das medidas de comprimento e das matérias fresca e seca dos caules. A salinidade tanto inibiu como retardou o crescimento dos caules. Os efeitos do NaCl nas atividades galactosidásicas de parede celular foram estudados in vivo e in vitro. A inibição e o retardamento do crescimento dos caules correlacionaram-se com as variações em atividades galactosidásicas. As galactosidases de parede celular de caules de plântulas tiveram suas atividades inibidas com o aumento da concentração de sal no meio de reação. A partir de 250 mM de NaCl as β-galactosidases foram mais sensíveis ao sal que α-galactosidases.In order to examine the participation of α- and β-galactosidases in the cell expansion of stems from cowpea seedlings submitted to salt stress during plant establishment as well as to analyze the effect of salt stress on the development of seedlings and enzymatic activities, Pitiúba cowpea seeds were sown in distilled water and in 100 mM NaCl. Throughout seed germination and seedling development, stems were harvested from seedlings at different stages of development and at different times after planting. Growth was evaluated by measuring stem length and fresh and dry mass of stems. Salinity both inhibited and delayed the growth of seedling stems. The effects of NaCl on galactosidase activities of the cell wall were studied, both in vivo and in vitro. Galactosidase activities in vivo were related to effects of NaCl inhibition and delay of stem development. The increase in salt concentration inhibited isolated galactosidase activity of cell wall from stems of seedlings. Starting at 250 mM NaCl, β-galactosidases were more sensitive to salt than α-galactosidases.

  17. Miguel Lisbona Guillén, Allí donde lleguen las olas del mar… / Pasado y presente de los chinos en Chiapas

    OpenAIRE

    Martínez, Rubén Torres

    2015-01-01

    Allí donde lleguen las olas del mar… Pasado y presente de los chinos en Chiapas, aborda desde una óptica nueva el fenómeno de la migración asiática, específicamente la china, en el estado mexicano de Chiapas. Hablamos de una óptica innovadora en tanto que la obra puede ser leída desde varias perspectivas. Puede servir como texto introductorio al fenómeno de la migración en México ; como estudio de caso de Chiapas ; igualmente puede ser leído como un tratado sobre el Estado mexicano y sus polí...

  18. Análisis contrastivo de los procesos de recategorización del adjetivo en español y en chino

    Directory of Open Access Journals (Sweden)

    M. A. Penas Ibáñez

    2013-10-01

    Full Text Available ENThe present contrastive study focuses on different processes of Metabasis in Spanish and Chinese. Although adjective category exists in both languages, its extension is different because this category is larger in Spanish. Consequently, there is no an intracategorial correspondence. Adjectives show affinities with substantives, with verbs and with adverbs. Normally in Chinese adjectives function like substantives and verbs; however, in Spanish adjectives usually function like substantives and adverbs. Therefore, we can see a partial coincidence. The use of Spanish adjectives as a noun presents three kinds of procedures: functional, lexicalizated and occasional, which affect to lexical and syntactic aspects. In Chinese these three kinds of procedures are too, but they affect to semantic aspects basically. In Spanish, only verbs can be predicates. In Chinese, besides of verbs, adjectives also can be predicates. Practically all Chinese adjectives can function like predicates. It is the main and more characteristic syntactic feature of the Chinese adjectives, which they don´t share with Spanish adjectives.<<>>El presente estudio contrastivo se ocupa de los procesos de recategorización del adjetivo en español y chino. Aunque en ambas lenguas existe la categoría del adjetivo, su extensión es diferente, siendo la del adjetivo español más grande; por lo que cada adjetivo español no va a tener su correspondencia con la categoría adjetiva en la lengua china, constatándose ausencia de paridad intracategorial. El adjetivo presenta afinidades con el sustantivo, con el verbo y con el adverbio. Generalmente, en chino, los adjetivos pueden desempeñar las funciones de dos categorías: la de sustantivo y la de verbo. En español, también se da este fenómeno, pero afecta fundamentalmente a las categorías de sustantivo y adverbio, por lo que se advierte una coincidencia parcial. La sustantivación de los adjetivos españoles presenta tres tipos de

  19. Overwintering of Uranotaenia Unguiculata Adult Females in Central Europe: A Possible Way of Persistence of the Putative New Lineage of West Nile Virus?

    Science.gov (United States)

    Rudolf, Ivo; Šebesta, Oldřich; Straková, Petra; Betášová, Lenka; Blažejová, Hana; VEnclíková, Kristýna; Seidel, Bernhard; Tóth, Sandor; Hubálek, Zdeněk; Schaffner, Francis

    2015-12-01

    We report the overwintering of Uranotaenia unguiculata adult females in Central Europe (Czech Republic, Hungary, Austria). This finding suggests a potential mode of winter persistence of putative novel lineage of West Nile virus in the temperate regions of Europe.

  20. 《今日汉语》(El Chino de Hoy)文化项目的分类及分布情况研究%On the Classification and Distribution of the Culture Elements in"El Chino de Hoy"

    Institute of Scientific and Technical Information of China (English)

    姚宁

    2016-01-01

    《今日汉语》(El Chino de Hoy)教材在西班牙语国家的汉语教学中占有十分重要的地位。文化项目是对外汉语教材的重要组成部分,文化内容在二语学习中占有重要的地位。本文对该教材的文化项目进行分类,在此基础上,分析子文化项目和文化项目群在三册教材中的分布情况,并提出建议。%The textbook,"El Chino de Hoy", occupies an important position in the Chinese teaching in Spanish speaking coun-tries. Culture element is a significant proportion in the textbook to teach Chinese as a foreign language, and so is cultural con-tent to learn a foreign language. Based on the classification of the culture elements in the textbook, the article analyses the dis-tribution of the sub-elements and elements groups in the series of three textbooks, and proposes suggestions.

  1. Cross species amplification of Adzuki Bean derived microsatellite markers in Asian Vigna species

    Directory of Open Access Journals (Sweden)

    M. Srimathy and P. Jayamani

    2010-07-01

    Full Text Available The Vigna is one of the important genus of grain legumes which forms the source of dietary protein and seven species of thisgenus, are domesticated as food crops in Asia. In recent years, molecular marker technology has greatly accelerated breedingprograms for the improvement of various crops. Among the different DNA markers, microsatellite or simple sequencerepeats (SSRs are the markers of choice for various genetic studies due to their co-dominant nature, loci specificity and highreproducibility. To date, only few reports are available on isolation and development of microsatellite markers in some of theVigna species. Therefore, the available SSR markers from other Vigna species should be validated for their transferabilityand utility in those species in which they are unavailable. In the present study, a set of 40 microsatellite primers pairs derivedfrom adzuki bean (Vigna angularis were used to assess the transferability and tested for their ability to amplifymicrosatellite loci in different species of Asian Vigna. The materials for this study included eleven different genotypesbelonging to seven species of Asian Vigna such as V. mungo var silvestris, V. mungo, V.umbellata, V. trilobata, V.aconitifolia, V. radiata var sublobata and V. radiata. All the 40 SSR primer pairs showed cross species amplification andproduced a total of 158 alleles in the genotypes studied. The percentage of amplification varied for each species whichranged from 37.5% (V.trilobata-2 to 100% (V. mungo var silvestris and V.mungo, while others showed more than 50%amplification. Apart from amplification, sufficient levels of polymorphism were also observed between cultivated blackgramand greengram and their progenitors V. mungo var silvestris and V. radiata var sublobata respectively. These findingssuggest that microsatellite markers from adzuki bean could be used in genomic studies of other Vigna species and thus aid intheir improvement.

  2. Del otro lado de la Gran Muralla. La transformación del sistema mediático chino (1979-2009, del modelo de propaganda a la prensa comercial

    Directory of Open Access Journals (Sweden)

    Salvador Salazar Navarro

    2012-01-01

    Full Text Available El presente artículo analiza los cambios acaecidos por el sistema chino de comunicación pública durante las últimas tres décadas. A partir de los presupuestos de la Teoría Social de la Comunicación, se abordan las relaciones comunicación/sociedad en la transición entre el paradigma mediático propio del socialismo real y el nuevo modelo comunicativo adoptado.

  3. Adzuki beans (Vigna angularis seed quality under several drying conditions

    Directory of Open Access Journals (Sweden)

    Osvaldo Resende

    2012-03-01

    Full Text Available This study analyzed the drying process and the seed quality of adzuki beans (Vigna angularis. Grains of adzuki beans, with moisture content of 1.14 (decimal dry basis at harvest and dried until the moisture content of 0.11 (decimal dry basis. were used. Drying was done in an experimental drier maintened at controlled temperatures of 30, 40, 50, 60, and 70 ºC and relative humidity of 52.0, 28.0, 19.1, 13.1, and 6.8%, respectively. Physiological and technological seed quality was evaluated using the germination test, Index of Germination Velocity (IGV, electrical conductivity, and water absorption, respectively. Under the conditions tested in the present study, it can be concluded that drying time for adzuki beans decreases with the higher air temperatures of 60 and 70 ºC, and it affected the physiological and technological seed quality. Thus, to avoid compromising adzuki seeds quality, it is recommended to promote its drying up to 50 ºC.

  4. Trypsin-chymotrypsin inhibitors from Vigna mungo seeds.

    Science.gov (United States)

    Cheung, Allen H K; Wong, Jack H; Ng, T B

    2009-01-01

    Three trypsin-chymotrypsin inhibitors were isolated from seeds of the black gram (Vigna mungo) with a procedure that entailed cation exchange chromatography on SP-Sepharose, anion exchange chromatography on Q-Sepharose, ion exchange chromatography by fast protein liquid chromatography (FPLC) on Mono Q and Mono S, and gel filtration by FPLC on Superdex 75. Two of the trypsin-chymotrypsin inhibitors were adsorbed on the first four types of chromatographic media. All three inhibitors have a molecular mass of 16 kDa as judged by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The trypsin inhibitory activity of the inhibitors was attenuated in the presence of the reducing agent dithiothreitol. The remaining inhibitor was unadsorbed on SP-Sepharose but adsorbed on Q-Sepharose, Mono Q and Mono S. The protease inhibitors did not exert any inhibitory effect on hepatoma (Hep G2) and breast cancer (MCF 7) cells or antifungal action toward Botrytis cinerea, Fusarium oxysporum and Mycosphaerella arachidicola. Two of the inhibitors slightly inhibited the activity of HIV-1 reverse transcriptase, with an IC50 in the millimolar range.

  5. Biosynthesis of alpha-Amylase in Vigna mungo Cotyledon.

    Science.gov (United States)

    Tomura, H; Koshiba, T

    1985-12-01

    In vitro translation of RNA extracted from Vigna mungo cotyledons showed that alpha-amylase is synthesized as a polypeptide with a molecular mass of 45,000, while cotyledons contain a form of alpha-amylase with a molecular mass of 43,000. To find out whether the 45,000 molecular mass polypeptide is a precursor to the 43,000 found in vivo, the cell free translation systems were supplemented with canine microsomal membrane; when mRNA was translated in the wheat germ system supplemented with canine microsomes, the 45,000 molecular mass form was not processed to a smaller form but the precursor form was partly processed in the membrane-supplemented reticulocyte lysate system. When V. mungo RNA was translated in Xenopus oocyte system, only the smaller form (molecular mass 43,000) was detected. Involvement of contranslational glycosylation in the maturating process of the alpha-amylase was ruled out because there was no effect of tunicamycin, and the polypeptide was resistant to endo-beta-H or endo-beta-D digestion. We interpret these results to mean that the 45,000 molecular mass form is a precursor with a signal peptide or transit sequence, and that the 43,000 molecular mass is the mature form of the protein.

  6. CHROMIUM INDUCED CYTOTOXICITY IN BLACKGRAM (VIGNA MUNGO L.

    Directory of Open Access Journals (Sweden)

    A. Chidambaram ، P. Sundaramoorthy ، A. Murugan ، K. Sankar Ganesh ، L. Baskaran

    2009-01-01

    Full Text Available Chromium is known to be highly toxic to biological systems. This study was designed to determine the mutagenic effects of different concentrations (0, 10, 25, 50, 100 and 200 mg/L of hexavalent chromium on root tip cells of blackgram (Vigna mungo L. Hepper. The blackgram seeds were equi-spacially arranged in sterilized petriplates lined with filter paper and they were treated with different concentrations of chromium solution. In germination studies, the morphological growth parameters such as germination percentage, root length, shoot length fresh weight and dry weight of blackgram seedlings were decreased with increasing dose of chromium concentrations. No germination of blackgram seeds was recorded at 300mg/l chromium concentration. Chromosome aberration assay was used to determine the mitotic indices and rate of chromosome aberration in blackgram root tip cells due to chromium treatment. The results showed that the mitotic indices were complicated due to different concentrations of chromium. However, the increase in chromium concentration has led to a gradual increase in the percentage of chromosomal aberration and mitotic index. The chromosome length, absolute chromosome length and average chromosome lengths were gradually found to decrease. There was no considerable change in 2n number of chromosome with the increase in chromium concentrations. It is concluded that the hexavalent chromium has significant mutagenic effect on the root tip cells of blackgram.

  7. Biosynthesis of α-Amylase in Vigna mungo Cotyledon 1

    Science.gov (United States)

    Tomura, Hideaki; Koshiba, Tomokazu

    1985-01-01

    In vitro translation of RNA extracted from Vigna mungo cotyledons showed that α-amylase is synthesized as a polypeptide with a molecular mass of 45,000, while cotyledons contain a form of α-amylase with a molecular mass of 43,000. To find out whether the 45,000 molecular mass polypeptide is a precursor to the 43,000 found in vivo, the cell free translation systems were supplemented with canine microsomal membrane; when mRNA was translated in the wheat germ system supplemented with canine microsomes, the 45,000 molecular mass form was not processed to a smaller form but the precursor form was partly processed in the membrane-supplemented reticulocyte lysate system. When V. mungo RNA was translated in Xenopus oocyte system, only the smaller form (molecular mass 43,000) was detected. Involvement of contranslational glycosylation in the maturating process of the α-amylase was ruled out because there was no effect of tunicamycin, and the polypeptide was resistant to endo-β-H or endo-β-D digestion. We interpret these results to mean that the 45,000 molecular mass form is a precursor with a signal peptide or transit sequence, and that the 43,000 molecular mass is the mature form of the protein. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:16664549

  8. Characterization of seed storage proteins of urdbean (Vigna mungo).

    Science.gov (United States)

    Mahajan, R; Malhotra, S P; Singh, R

    1988-01-01

    Dehulled and defatted flour of urdbean (Vigna mungo), Var T-9, contained 25% protein with maximum contribution by globulins (63%). Albumins and glutelins contributed 12% and 21% respectively, whereas prolamins were present only in traces (1%). Globulins were further fractionated into legumin and vicilin type proteins which were present in the ratio of 4:1. All the protein fractions were heterogenous in nature as revealed by high performance liquid chromatography. SDS-polyacrylamide gel electrophoresis revealed the total protein sample to contain 21 different components with molecular weights ranging from 8.92 to 117.49 kd. Albumins, globulins, prolamins and glutelins resolved into 4, 8, 6 and 13 different sized components of molecular weights ranging from 10.23 to 25.53, 10.84 to 112.72, 10.33 to 51.52 and 8.91 to 112.72 kd, respectively. Amino acid analysis of all fractions revealed that glutamic acid was present in maximum concentration followed by aspartic acid and lysine. Just like other pulse proteins, the urdbean proteins were also deficient in sulphur containing amino acids.

  9. Serrated leaf mutant in mungbean (Vigna radiata (L) Wilczek)

    International Nuclear Information System (INIS)

    Dry dormant seeds of mungbean (Vigna radiata (L) Wilczek) were treated with gamma rays (15, 30 and 60 kR). The serrated leaf mutation was noticed in M2 of cultivar Pak 32 treated with 60 kR. Cf 14 plants, 3 showed the altered leaf structure and the others were normal. The feature of this mutant was the deep serration of leaflet margins. The mutant had large thick leaflets with prominent venation. The mutant bred true in the M3 and successive generation. Details of the morphological characteristics of the mutant are presented. The mutant exhibited slower growth particularly during the early stages of development, flowered later and attained shorter height. There was an increase in the number of pods, in seed weight and in seed protein content, but number of seed per pod was considerably reduced. The seed coat colour showed a change from green to yellowish green. In the mutant's flowers the stamina were placed much below the stigma level and the stigma sometimes protruded the corolla. Outcrossing of 4% recorded in some of the mutant lines revealed a reduced cleistogamy. The low number of seeds per pod in the mutant could be due to reduced pollen fertility. The mutant behaved as monogenic recessive. The symbols SL/sl are proposed for this allelic pair. The mutant may have use as a green manure crop because of its large foliage and for the breeders as a genetic marker

  10. A revision of the genus Dysolobium (Papilionaceae) and the transfer of subgenus Dolichovigna to Vigna

    NARCIS (Netherlands)

    Welzen, van P.C.; Hengst, den S.

    1985-01-01

    Dysolobium is a small legume genus, consisting of 4 species. It is closely related to Vigna, from which it differs by the shape of the wing petals and by the velvety, almost woody pods. Recently the genus has been divided into 2 subgenera, but in this paper the subgenus Dolichovigna is transferred b

  11. Structure and expression of alpha-amylase gene from Vigna mungo.

    Science.gov (United States)

    Yamauchi, D; Takeuchi, H; Minamikawa, T

    1994-06-01

    A single copy of the alpha-amylase gene, composed of three introns and four exons, was found in Vigna mungo. Examination of levels of alpha-amylase and its mRNA in detached cotyledons indicated that attachment of the embryonic axis is not required for expression of the gene in cotyledons of germinating seeds.

  12. Photoperiod regulation of development and growth in bambara groundnut (Vigna subterranea).

    NARCIS (Netherlands)

    Linnemann, A.R.; Westphal, E.; Wessel, M.

    1995-01-01

    The influence of constant photoperiods of 10, 12, 14 and 16 h on development and growth in two bambara groundnut genotypes (Vigna subterranea (L.) Verdc., syn. Voandzeia subterranea (L.) Thouars) was studied in a greenhouse experiment in the Netherlands. Data on dry matter accumulation were collecte

  13. Potential biochemical markers for selection of disease resistance in Vigna radiata

    International Nuclear Information System (INIS)

    The Vigna radiata (L.) Wilczek (Green gram), a major pulse crop is prone to damaging diseases caused by Erysiphe polygoni, Cercospora canescens and Rhizoctonia sp. Therefore, the development of multiple resistance is a major breeding objective in green gram. Resistance to powdery mildew has already been developed, however, there are no reports on the development of resistance to Cercospora in green gram. Owing to limitation of conventional screening methods, the improvement for multiple disease resistance is inadequate, in this crop. It needs an efficient and quick selection method, for screening the plant population at an early stage. It is well established that the resistant interaction, in plants, involves accumulation of antibiotic compound phytoalexin (Genestein in Vigna radiata) and induction of enzymes such as β-1,3 gulcanase and Chitinases. These compounds are not only induced by pathogens but also pathogen-derived elicitors. These biochemical compounds can be used as resistance indicative biochemical markers for screening the natural or mutagen induced genetic diversity in populations of Vigna radiata in non-destructive manner. It, however, needs a systematic study of plant defense response. This paper deals with the response of resistant and susceptible cultivars of vigna radiata to Cercospora elicitor and development of non-destructive selection method for disease resistance. (author)

  14. Gamma Radiosensitivity Study on Long Bean (Vigna sesquipedalis

    Directory of Open Access Journals (Sweden)

    Ellyfa Kon

    2007-01-01

    Full Text Available This study was conducted to determine the LD50 and effect of gamma ray on germination percentage, plant height, survival percentage, root length, root dry weight and shoot dry weight of seedlings derived from seeds of long bean (Vigna sesquipedalis. Seeds of this crop were treated with 300, 400, 500, 600 and 800 Gy gamma rays at Malaysian Institute of Nuclear Technology (MINT. The treated seeds including control were sown in sand beds in size 4.6 x 0.7 m2 in a greenhouse at Horticulture Unit, Universiti Putra Malaysia Bintulu Campus (UPMKB. After germination, plants were transferred into polybags. Each polybag contained 1.2 kg blended growth medium with top soil, sand, and processed chicken dung ratio of 3:2:1. Standard procedures were used to observe and record the variables studied in this research. The experiment was designed as a 4 x 6 factorial Completely Randomized Design with 3 replications. Lethal dose 50 % of population (LD50 was assayed. The study revealed that germination percentage, plant height, survival percentage, root length, root dry weight and shoot dry weight decreased with increasing dose of gamma ray. The 800 Gy gamma ray dose in particular had a pronounced effect on these morphological characteristics probably because of injury it might have caused to the seeds of the long bean. As a result, poor growth and development was noticed. The LD50 for survival and height ranged between 600-800 Gy and 400-500 Gy, respectively. Generally, higher gamma ray doses particularly 800 Gy significantly affected the morphological characteristics of long bean seedlings obtained from irradiated seeds.

  15. Development of a black gram [Vigna mungo (L.) Hepper] linkage map and its comparison with an azuki bean [Vigna angularis (Willd.) Ohwi and Ohashi] linkage map.

    Science.gov (United States)

    Chaitieng, B; Kaga, A; Tomooka, N; Isemura, T; Kuroda, Y; Vaughan, D A

    2006-11-01

    The Asian Vigna group of grain legumes consists of six domesticated species, among them black gram is widely grown in South Asia and to a lesser extent in Southeast Asia. We report the first genetic linkage map of black gram [Vigna mungo (L.) Hepper], constructed using a BC(1)F(1) population consisting of 180 individuals. The BC(1)F(1) population was analyzed in 61 SSR primer pairs, 56 RFLP probes, 27 AFLP loci and 1 morphological marker. About 148 marker loci could be assigned to the 11 linkage groups, which correspond to the haploid chromosome number of black gram. The linkage groups cover a total of 783 cM of the black gram genome. The number of markers per linkage group ranges from 6 to 23. The average distance between adjacent markers varied from 3.5 to 9.3 cM. The results of comparative genome mapping between black gram and azuki bean show that the linkage order of markers is highly conserved. However, inversions, insertions, deletions/duplications and a translocation were detected between the black gram and azuki bean linkage maps. The marker order on parts of linkage groups 1, 2 and 5 is reversed between the two species. One region on black gram linkage group 10 appears to correspond to part of azuki bean linkage group 1. The present study suggests that the azuki bean SSR markers can be widely used for Asian Vigna species and the black gram genetic linkage map will assist in improvement of this crop.

  16. Genetic diversity analysis in blackgram (Vigna mungo (L.) Hepper) using AFLP and transferable microsatellite markers from azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi).

    Science.gov (United States)

    Gupta, S K; Gopalakrishna, T

    2009-02-01

    Genetic diversity in 20 elite blackgram (Vigna mungo (L.) Hepper) genotypes was studied using microsatellite and AFLP markers. Thirty-six microsatellite markers from azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi) were successfully amplified across the 20 blackgram genotypes and 33 microsatellite markers showed polymorphism. A total of 137 microsatellite alleles were generated with an average of 4.1 alleles per locus. The number of alleles ranged from two to nine and the polymorphic information content value for the microsatellite markers varied from 0.10 to 0.87 with an average of 0.49. Microsatellite markers were highly informative and a combination of only three microsatellite markers (CEDG264, CEDG173, and CEDG044) was sufficient to discriminate all 20 blackgram genotypes. In the case of AFLP, 11 primer pairs generated 324 polymorphic marker fragments. The polymorphic information content values for AFLP primer combinations ranged from 0.21 to 0.34 with an average of 0.29. Similarity measures and clustering analyses were made using microsatellite and AFLP data separately. The resulting dendrograms distributed the 20 blackgram genotypes into five main clusters. The dendrograms were comparable with each other with the Mantel test between the cophenetic matrices of microsatellite data and AFLP data showing moderate correlation (r = 0.64). The results of the principal components analysis were well congruent with the dendrograms. In the dendrograms as well as in the principal components analyses, genotype Trombay wild (Vigna mungo var. silvestris) was placed separately from rest of the genotypes. This study demonstrated that the azuki bean microsatellite markers are highly polymorphic and informative and can be successfully used for genome analysis in blackgram. Results indicate that sufficient variability is present in the blackgram genotypes and would be helpful in the selection of suitable parents for breeding purposes and gene mapping studies.

  17. COMBINED EFFECTS OF PLANT GROWTH PROMOTING RHIZOBACTERIA AND FUNGI ON MUNG BEAN (VIGNA RADIATA L.

    Directory of Open Access Journals (Sweden)

    Ravi Kumar Gangwar, Gaurav Bhushan Jaspal Singh *, Sudhir K. Upadhyay and A.P. Singh

    2013-11-01

    Full Text Available In the present study, screened PGPR and Fungi were influence the growth of Mung bean (Vigna radiata plant in the pot. Two rhizobacteria viz. Rhizobium sp., Pseudomonas putida and three fungi Aspergillus niger, Rhizopus sp. and Trichoderma viride were isolated and purified. The effect of inoculation of different strains of bacteria and fungus on growth responses of Vigna radiata under pot condition was enumerated. The result revealed that the single and dual inoculation of these microbial strains enhances the plant growth in terms of root and shoot length and dry-biomass. The maximum increase in root length (up to 86.57%, shoot length (up to 56.91%, root dry weight (up to 94.42%, and shoot dry weight (up to 56.09% was observed in response to dual inoculation of Pseudomonas putida with Trichoderma viride compared to uninoculated control.

  18. EVALUATION OF SEDATIVE ACTIVITY OF AQUEOUS EXTRACT OF VIGNA TRILOBATA (L. VERDC. LEAVES

    Directory of Open Access Journals (Sweden)

    Ahir Chetan D

    2011-04-01

    Full Text Available The aim of present study is to evaluate the sedative activity of aqueous extract of Vigna trilobata (L verdc. Leaves using experimental animal models. In the present study aqueous extract of the Vigna trilobata leaves (AEVTL was used to investigate the sedative activity using Rotarod apparatus and Photoactometer in mice at a dose of 200 and 400 mg/kg of body weight and compared to standard diazepam (5mg/kg, i.p.. The result obtained from this study revealed that AEVTL possessed significant (p<0.05 and p<0.01 sedative activity at a dose of 200 and 400 mg/kg by reducing locomotor activity and fall off time in mice in a dose dependent manner. The results of this study justify the use of the leaves as sedative in traditional medicine. Further studies may be directed at characterizing the bioactive ingredients that are responsible for the observed sedative activity in the plant.

  19. Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence

    OpenAIRE

    Neha Gupta; Nidhi Shrivastava; Pramod Kumar Singh; Bhagyawant, Sameer S.

    2016-01-01

    In the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia) seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of photochemical composition was correlated with seed storage proteins like albumin and globulin. Qualitative identification of total seed storage protein abundance across two related moth bean acces...

  20. Inheritance of resistance to yellow mosaic virus in blackgram (Vigna mungo (L.) Hepper).

    Science.gov (United States)

    Singh, D P

    1980-09-01

    The inheritance of resistance to mungbean yellow mosaic virus (MYMV) was studied in blackgram (Vigna mungo (L.) Hepper). The highly resistant donors Pant U-84 and UPU-2 and a highly susceptible line, UL-2, their F1's, F2's and backcrosses were grown with spreader located every 5 to 6 rows. The resistance was found to be digenic and recessive in all the crosses and free from cytoplasmic effect.

  1. Measurement of nitrite reductase in leaf tissue of Vigna mungo : A new method.

    Science.gov (United States)

    Srivastava, R C; Bose, B; Mukerji, D; Mathur, S N; Srivastava, H S

    1979-12-01

    The enzyme nitrite reductase (EC 1.6.6.4) is generally assayed in terms of disappearance of nitrite from the assay medium. We describe a technique which allowed estimation of the enzyme level in leaf tissues of Vigna mungo (L). Hepper in terms of the release of the product (NH3) of the enzyme reaction. The technique is offered as an alternative, possibly more convenient method for assay of nitrite reductase in plant tissue in vivo.

  2. Comparative Studies of Nitrogen Fixing Potential of Desmodium ramississimon and Vigna unquiculata for Soil Fertility Management

    OpenAIRE

    Ngwu, OE.

    2005-01-01

    The occurrence of large numbers of legume species in the tropics with potentials for nitrogen fixation could be exploited to supply nitrogen, if they can be integrated into the farming system. The N2 – fixing potential of a native herbaceous leguminous species namely, Desmodium ramississimon (Dm) and grain legume, Vigna unquiculata (Cp) were studied in the green house and field, on three types of soil. In both situations, nodulation was influenced by the soil type. Nsukka soil, which had sand...

  3. Changes in nutrient and antinutrient composition of Vigna racemosa flour in open and controlled fermentation.

    Science.gov (United States)

    Difo, V H; Onyike, E; Ameh, D A; Njoku, G C; Ndidi, U S

    2015-09-01

    This study was conducted to investigate the effect of open and controlled fermentation on the proximate composition, mineral elements, antinutritional factors and flatulence-causing oligosaccharides in Vigna racemosa. The open fermentation was carried out using the microorganisms present in the atmosphere while the controlled fermentation was carried out using Aspergillus niger as a starter. The proximate composition of the Vigna racemosa, some anti-nutrients and the mineral elements were analyzed using standard procedures. The protein content was increased by 12.41 ± 1.73 % during open fermentation while it decreased by 29.42 ± 0.1 % during controlled fermentation. The lipids, carbohydrates, crude fibre and ash content were all reduced in both types of fermentation except the moisture content which increased in controlled fermentation. Apart from calcium, the other elements (Fe, Na, Mg, Zn, and K) suffered reduction in both types of fermentation. The phytate, tannin, alkaloids, hydrogen cyanide, lectins, trypsin inhibitors and oxalate content all had drastic reductions in both types of fermentation. Open and controlled fermentation reduced the levels of both raffinose and stachyose. The percentages of reduction due to controlled fermentation were higher than those of open fermentation in the antinutrients studied. Fermentation is an efficient method for detoxifying the antinutrients in the Vigna racemosa studied in this work. PMID:26345026

  4. Analysis of Genetic Variation of Seed Proteins in the Genus Vigna and among Its Relatives Cultivated in China

    Institute of Scientific and Technical Information of China (English)

    CHEN Chanyou; PAN Lei; HU Yaojun; HU Zhihui; DING Yi

    2006-01-01

    The genetic variation of seed proteins was assayed by SDS-PAGE foR24 cultivars belonging to 5 species in Vigna and 7 species in its 7 relative genera cultivated in China. There were 48 polymorphic subunit bands discriminated from electrophoretic profiles. Two dendrograms were constructed by UPGMA cluster analyses using PHYLIP3.6 respectively.Variation among genera or species was larger than that among lower taxonomic categories level. Little variation among cultivars of yardlong bean (Vigna sesquipedalis ) and small variation of lablab (Lablab purpureus ),pea (Pisum sativum ), or sword bean (Canavalia gladiata ), but large variation of soybean or rice bean in their origin of China were all revealed.The seed proteins profiles of traditionally regarded as typical species in Vigna such as yardlong bean, rice bean and small bean were more similar than mungbean (Vigna radiata) and black gram (Vigna mungo) were.Mungbean and black gram had distinct seed proteins pattern, they should be of two species.

  5. Effect of Crude Brassinosteroid Extract on Growth and Biochemical Changes of Gosssypium hirsutum L. and Vigna mungo L.

    Directory of Open Access Journals (Sweden)

    Syed Ali Fathima M

    2011-12-01

    Full Text Available The present study was aimed to examine the influence of BRs on seed germination and seedling growth in Gossypium hirsutum L. Var Svpr 2 and Vigna mungo (L. Hepper Var T9. The application of BRs on seed germination of Gossypium hirsutum increased the rate of germination considerably with varied percentage from 60.4 to 99. Vigna mungo seed also showed the varied percentage of germination from 56.8 to 80.1. Both the plants exhibited high percentage of vegetative growth such as shoot length, fresh weight, dry weight and leaf area on 3% of BR supplementation. The amount of chlorophyll a, b and total chlorophyll increased under BR treatments. Among the concentration, 3% BRs caused maximum effect than the other tested concentrations. High percentage of starch 53% and 31 % was observed in Gossypium and Vigna mungo respectively. The results of the present study shows that 3% BRs promotes the growth rate of Gossypium hirsutum L. Var Svpr 2 and Vigna mungo (L. Hepper Var. T9. The results of the present study supplemented to the previous observations and practical utilization of the new steroidal group of phytohormones for large scale production of the economically important crops Gossypium hirsutum L. Var Svpr 2 and Vigna mungo (L. Hepper Var. T9.

  6. Bioactivity of Powder and Extracts from Garlic, Allium sativum L. (Alliaceae) and Spring Onion, Allium fistulosum L. (Alliaceae) against Callosobruchus maculatus F. (Coleoptera: Bruchidae) on Cowpea, Vigna unguiculata (L.) Walp (Leguminosae) Seeds

    OpenAIRE

    Abiodun A. Denloye

    2010-01-01

    Laboratory bioassays were conducted to investigate the bioactivity of powders, extracts, and essential oils from Allium sativum L. (Alliaceae) and A. fistulosum L. (Liliaceae) against adults, eggs, and larvae of Callosobruchus maculatus F. (Coleoptera: Bruchidae). On the basis of 48 hr median lethal toxicity (LC50), test plant powders and extracts from A. sativum were more toxic to C. maculatus adults than those from A. fistulosum. The 48 hr LC50 values for the powder against the test insect ...

  7. Bioactivity of Powder and Extracts from Garlic, Allium sativum L. (Alliaceae and Spring Onion, Allium fistulosum L. (Alliaceae against Callosobruchus maculatus F. (Coleoptera: Bruchidae on Cowpea, Vigna unguiculata (L. Walp (Leguminosae Seeds

    Directory of Open Access Journals (Sweden)

    Abiodun A. Denloye

    2010-01-01

    Full Text Available Laboratory bioassays were conducted to investigate the bioactivity of powders, extracts, and essential oils from Allium sativum L. (Alliaceae and A. fistulosum L. (Liliaceae against adults, eggs, and larvae of Callosobruchus maculatus F. (Coleoptera: Bruchidae. On the basis of 48 hr median lethal toxicity (LC50, test plant powders and extracts from A. sativum were more toxic to C. maculatus adults than those from A. fistulosum. The 48 hr LC50 values for the powder against the test insect species were 9.66 g/kg and 26.29 g/kg for A. sativum and A. fistulosum, respectively. Also the 48 hr LC50 values obtained show that aqueous extracts of the test plant species, 0.11 g/L (A. sativum and 0.411 g/L (A. fistulosum were more toxic to C. maculatus than the corresponding ethanol extracts. There was no significant difference in the toxicity of vapours from the two test plant species against C. maculatus, although A. sativum gave lower values. The study shows that A. sativum and A. fistulosum have potentials for protecting stored cowpea from damage by C. maculatus.

  8. EFEITO DO PROCESSAMENTO SOBRE OS TEORES DE OXALATO E TANINO EM MAXIXE (CUCUMIS ANGURIA L., JILÓ (SOLANUM GILO, FEIJÃO VERDE (VIGNA UNGUICULATA (L. WALP E FEIJÃO ANDU (CAJANUS CAJAN (L. MILL SP

    Directory of Open Access Journals (Sweden)

    Clícia Maria de Jesus BENEVIDES

    2013-08-01

    Full Text Available Este trabalho objetivou avaliar o efeito do processamento térmico e do armazenamento em salmoura ácida, em função do tempo, nos teores de substâncias antinutricionais em hortaliças (maxixe e jiló e leguminosas (feijão verde e feijão andu comercializadas em Salvador- -BA. Os teores de oxalatos (ox e taninos (tan foram determinados nos vegetais in natura, submetidos ao branqueamento (100°C/3min e nos vegetais em salmoura ácida, hermeticamente fechados e armazenados, durante um período de seis meses. A determinação de oxalato foi realizada por permanganametria, utilizando solução de KMnO4 (0,002mol.L-1 e os taninos foram quantificados segundo o método de Folin Ciocalteau. Os valores médios (mg/100g encontrados nos vegetais in natura foram:maxixe (tan: 1,56±0,00 e ox: 1,44±0,00 e jiló (tan:3,46±0,04 e ox: 3,58±0,08. Com relação às leguminosas, os valores mé- dios (mg/100g foram: feijão verde (tan: 4,71±0,08 e ox: 4,92±0,03 e feijão andu (tan:6,05±0,02 e ox: 0,48±0,04. Houve redução média de 17,5% (ox e 10% (tan após o tratamento térmico e de 60,75% (ox e 89,2 % (tan após o armazenamento dos vegetais estudados, quando comparados com os teores dos mesmos na forma in natura. Conclui-se que os teores dos fatores antinutricionais diminuem após tratamento térmico e durante o armazenamento desses produtos em conservas.

  9. Comportamiento de Rhizoctonia solani kühn en siembras realizadas durante todo el año, en el cultivo de Vigna unguiculata L. Walp. (fríjol caupí

    Directory of Open Access Journals (Sweden)

    Vilma López Cruz

    2010-01-01

    Full Text Available Se evaluó el comportamiento de Rhizoctonia solani Kühn frente a diferentes variedades de Fríjol Caupí en siembras mensuales realizadas durante los años 2004 y 2005 en las áreas experimentales de la Estación Territorial de Investigaciones Agropecuarias de Holguín (ETIAH, con el objetivo de determinar la época optima de siembra del cultivo, en correspondencia con los porcentajes de incidencia del patógeno y los rendimientos obtenidos, se utilizaron las variedades Carita tradicional, Titán, INIFAT-93 e IITA-Precoz, todas de categoría R1. La evaluación de la enfermedad se realizó según las metodologías del Instituto Nacional de Investigaciones de Sanidad Vegetal (INISAV, Cuba 1989 y para el análisis de las plantas afectadas por el patógeno se utilizó una escala de grado modificada propuesta por el Centro Internacional de Agricultura Tropical (CIAT, Colombia 1987. Los rendimientos obtenidos fueron procesados mediante análisis de varianza (Lerch, 1987 y prueba de Newman- Keuls, con el auxilio del paquete estadístico STATITCF (versión 4,0. De los resultados alcanzados se infiere que las variedades Titán y la IITAPrecoz fueron las de mayor tolerancia al patógeno, mientras que la Carita tradicional y la INIFAT- 93 fueron las más susceptible y en cuanto a las fechas de siembra evaluadas las de Abril y Mayo resultaron ser las óptimas para el cultivo, al lograrse los menores porcentajes de incidencia del patógeno y los mayores valores de cosecha.

  10. Effect of Pb2+ Stress on Vigna unguiculata Seeds Germination and Seedlings Growth%重金属铅对豇豆种子的萌发及幼苗生长的影响

    Institute of Scientific and Technical Information of China (English)

    赵淑玲; 王瀚; 赵桂芳; 卓平清

    2016-01-01

    试验用不同浓度Pb2+(100,200,400,600 mg/L)处理豇豆种子,研究豇豆种子萌发、生根以及叶绿素、SOD、POD等生理生化指标变化.结果表明:随pb2+浓度的增加,豇豆种子的萌发率不受影响,但主根生长、根毛发生、叶绿素含量受抑制,SOD、POD活性先升高后下降.

  11. Effects of low temperatures on seed germination and seedling emergence of cowpea (Vigna unguiculata)%低温对豇豆种子萌发和出苗的影响

    Institute of Scientific and Technical Information of China (English)

    张献英; 唐力生; 犹昌艳; 胡飞

    2013-01-01

    [目的]研究低温对豇豆种子萌发及出苗的影响,为华南地区冬种豇豆拒避低温影响提供参考.[方法]以油白1号和粤红5号豇豆种子为试材,在人工气候箱内研究不同温度(10、15、20和25℃)、不同温度组合和不同温度持续时间长短对种子萌发和出苗的影响.[结果]不同温度处理试验结果显示,10℃时两个豇豆品种起始萌发比25℃滞后12~13 d,油白1号的萌发率为33.3%,粤红5号为63.0%;15℃时两个品种起始出苗时间比25 ℃滞后9~10d,油白1号的出苗率为22.0%,粤红5号为18.0%; 10℃时两品种均未能出苗.在低温处理不同时间条件下,10℃低温持续5 d以上种子萌发率显著降低,油白1号为74.1%,粤红5号为44.4%;出苗过程中10℃低温持续2d以上或15℃低温持续5d以上,种子出苗率显著降低.油白1号在相同低温条件下比粤红5号的抗性表现略好.[结论]在华南地区冬种豇豆如果出现10℃低温的天气,或15℃以下低温天气持续5 d以上不宜进行播种;若播种后10℃低温持续2 d以上或15 ℃低温持续5d以上,要采取保温等措施,确保较高的出苗率.

  12. 温度胁迫对豇豆种子萌发生理指标的影响%The Effect of Temperature Stress on Seed Germination Physiological Indices in Asparagus Bean(Vigna unguiculata L.ssp.sesquipdalis Verdc.)

    Institute of Scientific and Technical Information of China (English)

    万茜; 刘伟; 陈禅友

    2007-01-01

    以3个豇豆品种为材料,采用完全随机设计,重复3次,设置低温15℃、常温25℃(对照)和高温35℃3个处理,进行种子萌发实验.确定供试种子批种子活力正常后,在萌发种子胚根长到1cm时,分胚根和子叶两个部位取样测定其可溶性蛋白质含量(TSP)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性等生理指标.结果表明:温度胁迫使胚根中TSP含量均较常温显著或极显著降低,子叶中的TSP含量在低温处理下极显著高于对照,而在高温下与之相反;温度胁迫下,各豇豆品种胚根和子叶中MDA含量均较显著或极显著高于对照;SOD活性变化与之相反,但品种间有差异.胚根POD活性在低温下极显著高于对照,在高温下却显著或极显著低于对照,而子叶中与之相反.子叶中CAT活性在低温下上升,高温下下降,而胚根中均上升.因此,低温和高温都明显阻碍豇豆种子萌发,但生理基础上不完全一致,且胚根和子叶的反应有明显差别,其中比较一致的是种胚萌发时MDA含量升高和SOD活性下降,反应不一致的有TSP含量、POD活性及CAT活性变化.

  13. Effect of Simulated Acid Rain on Seed Germination and Seedling Growth in Vigna unguiculata ssp. sesquipedatis (L.) Verde%模拟酸雨对豇豆种子萌发及幼苗生长的影响

    Institute of Scientific and Technical Information of China (English)

    韩春梅

    2009-01-01

    研究了不同pH值(1.0,2.0,3.0,4.0,5.0和6.5)酸雨时豇豆种子萌发及幼苗生长的影响.研究结果表明,随着pH值的下降,豇豆的发芽率、发芽势、发芽指数、幼苗根长及下胚轴长均呈降低的趋势,均受到不同程度的抑制.当pH值降到最低1.0(酸度最强)时,所有豇豆种子全部发生霉烂现象.

  14. Purification, properties and alternate substrate specificities of arginase from two different sources: Vigna catjang cotyledon and buffalo liver

    OpenAIRE

    Dabir, Snehal; Dabir, Pankaj; Somvanshi, Baburao

    2005-01-01

    Arginase was purified from Vigna catjang cotyledons and buffalo liver by chromatographic separations using Bio-Gel P-150, DEAE-cellulose and arginine AH Sepharose 4B affinity columns. The native molecular weight of an enzyme estimated on Bio-Gel P-300 column for Vigna catjang was 210 kDa and 120 kDa of buffalo liver, while SDS-PAGE showed a single band of molecular weight 52 kDa for cotyledon and 43 kDa for buffalo liver arginase. The kinetic properties determined for the purified cotyledon a...

  15. Eficiência e diversidade fenotípica de bactérias diazotróficas que nodulam caupi [Vigna unguiculata (L. Walp] e feijoeiro (Phaseolus vulgaris L. em solos de mineração de bauxita em reabilitação Efficiency and phenotypic diversity among nitrogen-fixing bacteria that nodulate cowpea [Vigna unguiculata (L. Walp] and common bean (Phaseolus vulgaris L. in bauxite-mined soils under rehabilitation

    Directory of Open Access Journals (Sweden)

    Rogério Melloni

    2006-04-01

    Full Text Available A atividade de mineração provoca degradação ambiental em várias partes do mundo, e técnicas de revegetação, empregando diversas espécies vegetais, têm sido indicadas, com vistas em reabilitar essas áreas. A eficiência e a diversidade de grupos-chave de bactérias, como as que nodulam leguminosas e fixam N2 (BNLFN, são de extrema importância, uma vez que estas participam de processos de ciclagem de nutrientes e contribuem para a sustentabilidade dessas áreas. Com o objetivo de avaliar a eficiência e a diversidade de BNLFN, coletaram-se amostras de solo de áreas mineradas, sob diferentes estratégias e cronosseqüências de reabilitação, no verão de 1999, para instalação de ensaios, empregando feijoeiro e caupi como espécies de plantas-isca de BNLFN. Na floração, coletaram-se as plantas e avaliaram-se matéria seca da parte aérea, número, matéria fresca e atividade de nódulos. Não houve influência das diferentes estratégias de reabilitação na eficiência das populações de BNLFN no crescimento do feijoeiro. A diversidade fenotípica dos isolados de BNLFN foi avaliada por meio das características culturais destes em meio de cultivo 79. Após a caracterização fenotípica de 328 isolados de feijoeiro e 420 de caupi, verificou-se que este é mais indicado que o feijoeiro nos estudos de nodulação, eficiência e diversidade de BNLFN. O impacto negativo da mineração é maior na diversidade fenotípica cultural de BNLFN do que na nodulação das plantas-isca utilizadas. A revegetação contribuiu para aumentar a diversidade dessas bactérias em solos de áreas de mineração de bauxita, especialmente quando ocorreu a introdução de leguminosas.Mining activities promote severe environmental degradation in many parts of the world, and revegetation techniques with diverse plant species have been used for rehabilitation of these areas. Studies on the occurrence, efficiency and diversity of key microbial groups such as leguminosae-nodulating, nitrogen fixing bacteria (LNNFB are essential because they are involved in nutrient cycling processes and, therefore, to the sustainability of these areas. Aiming to evaluate the efficiency and phenotypic diversity of LNNFB, soil samples were collected from different bauxite mining areas with distinct rehabilitation strategies and chronosequences in the summer of 1999. With these materials we installed experiments using bean and cowpea as trap species under greenhouse conditions. At flowering, the plants were harvested and the shoot dry weight, nodule number, nodule weight, nitrogenase activity, and phenotypic diversity of LNNFB isolates evaluated by culture characterization on 79 medium. There was no influence of the different rehabilitation strategies on the efficiency of LNNFB populations in promoting bean growth. After phenotypic characterization of 328 bean and 420 cowpea LNNFB isolates, it was found that the latter is better suited than bean for studies evaluating LNNFB nodulation, efficiency and diversity in these areas. Mining strongly decreases the LNNFB diversity, while there is little effect on nodulation of trap plants. Rehabilitation strategies contribute to increase LNNFB diversity in bauxite mined soils, mainly when legume species were introduced.

  16. Evaluation of anti-osteoarthritic activity of Vigna mungo in papain induced osteoarthritis model

    OpenAIRE

    Patel, Dhaval V.; Mrunal Ghag Sawant; Ginpreet Kaur

    2015-01-01

    Aim: This study was carried out to evaluate the effect of Vigna mungo hydroalcoholic extract (VMHA) by papain induced osteoarthritis (OA) in the rat model. Materials and Methods: OA was induced by intra-articular injection of papain (4% w/v) along with cysteine (0.03 M) on day 1, 4 and 7 in rats and VMHA was administered orally in three doses (100, 200 and 400 mg/kg) after last papain injection. The anti-osteoarthritic activity was evaluated by measuring knee joint diameter, grip strength...

  17. biostudy on vigna sinensis beetle, callosobruchus chinensis l.and its control by gamma radiation

    International Nuclear Information System (INIS)

    the present study carried out to determine: 1.the effects of the separate and combined effect of bacillus thuringiensis and gamma radiation on some biological aspects of callosobruchus chinensis. 2. the weight loss in infected seeds and total population of the pest after store it for different on adult beetles to determine the proteins, lipids and carbohydrates contents. 4. some analysis in the cowpea vigna anguiculata seeds to investigate the nutritive value after different treatments with bacillus thuringiensis (b.t) and gamma radiation

  18. Antioxidant Status of Vigna mungo L. in Response to Sulfur Nutrition

    OpenAIRE

    Neelam Chandra; Nalini Pandey

    2014-01-01

    Black gram (Vigna mungo L. var. DPU-88-31), an edible legume, was grown at 1, 2, 4, 6, and 8 meq S L−1 to study the effect of deficient and excess level of sulfur on oxidative metabolism. Plants supplied by 4 meq S L−1 showed optimum yield. Sulfur deficient plants (1 and 2 meq S L−1) showed reduction in growth and chlorosis of young leaves. Tissue sulfur and cysteine concentration was increased with increasing sulfur supply. The thresholds for critical concentration of sulfur deficiency and t...

  19. Temporal variation in protein content and yield of Vigna mungo (L.) Hepper leaves.

    Science.gov (United States)

    Pandey, V N; Srivastava, A K

    1990-10-01

    Temporal variation in total protein and soluble protein contents and protein yield of Vigna mungo leaves at intervals of every three hours during day and night was studied. The study was done with the view to ascertain the hour of harvesting the leaves for maximum yield of leaf protein concentrate. Observations reveal that the total protein and soluble protein contents in the leaves are minimum during 3.00 to 6.00 hrs, which steadily rise with time to reach the maximum values during 12.00 to 15.00 hrs, after which the same shows a steady decrease with time.

  20. Analyses of MYMIV-induced transcriptome in Vigna mungo as revealed by next generation sequencing

    Directory of Open Access Journals (Sweden)

    Sayak Ganguli

    2016-03-01

    Full Text Available Mungbean Yellow Mosaic Virus (MYMIV is the viral pathogen that causes yellow mosaic disease to a number of legumes including Vigna mungo. VM84 is a recombinant inbred line resistant to MYMIV, developed in our laboratory through introgression of resistance trait from V. mungo line VM-1. Here we present the quality control passed transcriptome data of mock inoculated (control and MYMIV-infected VM84, those have already been submitted in Sequence Read Archive (SRX1032950, SRX1082731 of NCBI. QC reports of FASTQ files generated by ‘SeqQC V2.2’ bioinformatics tool.

  1. Analyses of MYMIV-induced transcriptome in Vigna mungo as revealed by next generation sequencing.

    Science.gov (United States)

    Ganguli, Sayak; Dey, Avishek; Banik, Rahul; Kundu, Anirban; Pal, Amita

    2016-03-01

    Mungbean Yellow Mosaic Virus (MYMIV) is the viral pathogen that causes yellow mosaic disease to a number of legumes including Vigna mungo. VM84 is a recombinant inbred line resistant to MYMIV, developed in our laboratory through introgression of resistance trait from V. mungo line VM-1. Here we present the quality control passed transcriptome data of mock inoculated (control) and MYMIV-infected VM84, those have already been submitted in Sequence Read Archive (SRX1032950, SRX1082731) of NCBI. QC reports of FASTQ files generated by 'SeqQC V2.2' bioinformatics tool.

  2. Gamma ray induced physiological variabilities in black gram (Vigna mungo L. )

    Energy Technology Data Exchange (ETDEWEB)

    Ram Mohan Rao, S.; Rao, D. (Kakatiya Univ., Warangal (India). Dept. of Botany)

    1983-06-01

    Various doses of gamma rays were used to irradiate dry seeds of Vigna mungo. 20KR induced increased seedling growth and lateral root number, while the 60 KR reduced them. Fresh and dry weights of whole seedlings were retarded with increasing dose rate. However, more fresh and dry weight values were recorded in the 20 KR treatment. Plant height, number of nodes, internodal length and yield were found to be favourably induced in the lower dose while in the higher doses particularly in 50 and 60 KR there was a reduction. But a reverse situation was found regarding chlorophyll metabolism in the foliage leaves.

  3. A monomeric protein with hemagglutinating activity from seeds of Vigna mungo (Phaseolus mungo).

    Science.gov (United States)

    Singh, S S; Rao, S L

    1991-01-01

    Black gram (Vigna mungo) seeds are shown to contain a lectin with certain unusual features. The lectin agglutinates only trypsinized red cells, and its sugar specificity is complex as none of the common sugars, oligosaccharides or complex polysaccharides exhibit any affinity for the lectin. The purified lectin has a molecular weight of 58 kDa and is a monomer. Unlike other plant lectins, antibodies to the P. mungo lectin do not exhibit any immunological cross reactivity. The clot forming ability of the lectin is unusual in that the clot once formed is rapidly disaggregated indicated that it induces, as yet undefined, certain membrane alterations.

  4. Production and composition of extracellular polysaccharide synthesized by a Rhizobium isolate of Vigna mungo (L.) Hepper.

    Science.gov (United States)

    Mandal, Santi Mohan; Ray, Bimalendu; Dey, Satyahari; Pati, Bikas Ranjan

    2007-08-01

    An extracellular polysaccharide (EPS) was produced by a Rhizobium sp. isolated from the root nodules of Vigna mungo (L.) Hepper. Maximum EPS production (346 mg l(-1)) was when the yeast extract basal medium was supplemented with mannitol (1%), biotin (1.5 mg l(-1)) and asparagine (0.3%). Ribose (53%) and mannose (47%) were the principle monomers of the EPS. Chemical, chromatographic and spectroscopic analysis showed that this polymer, which has Man(4)Rib(1) as an oligomeric subunit, has an apparent molecular mass of 750 kDa.

  5. Induction of chlorophyll chimeras and chlorophyll mutations in mungbean (Vigna radiata) cv. T44

    International Nuclear Information System (INIS)

    Uniform and healthy seeds of mungbean (Vigna radiata) cv. T44 were exposed to varying doses of gamma rays, ethyl methane sulphonate (EMS) and combination treatment of gamma rays with EMS. The data were recorded for seed germination, plant survival, frequency and spectrum of chlorophyll chimeras in M1 and chlorophyll mutations in M2 generation. Among all, the combination treatments were found most effective for inducing chlorophyll chimeras and chlorophyll mutations than the gamma rays or EMS alone. Of the mutants under reference, the albino, xantha and chlorina showed monogenic recessive while viridis exhibited digenic recessive inheritance. (author). 8 refs., 2 tabs

  6. Growth responses of Vigna radiata seeds to laser irradiation in the UV-A region

    International Nuclear Information System (INIS)

    We have investigated the effect of pulsed nitrogen laser radiation (337.1 nm) on morphological characteristics and biochemical contents in seedlings from treated greengram (Vigna radiata L.) seeds, which were germinated and grown in Petri dishes for a week. The shoot and root lengths, and fresh and dry weights of the seedlings were maximum with the 30 min exposure, while protein was maximum with 20 min, RNA and DNA contents with 5 min exposure time. Chlorophyll content was not affected by the irradiation

  7. De novo transcriptome assembly of two Vigna angularis varieties collected from Korea

    OpenAIRE

    Jo, Yeonhwa; Lian, Sen; Cho, Jin Kyong; Choi, Hoseong; Kim, Sang-Min; Kim, Sun-Lim; Lee, Bong Choon; Cho, Won Kyong

    2016-01-01

    The adzuki bean (Vigna angularis), a member of the family Fabaceae, is widely grown in Asia, from East Asia to the Himalayas. The adzuki bean is known as an ingredient that adds sweetness to diverse desserts made in Eastern Asian countries. Libraries prepared from two V. angularis varieties referred to as Taejin Black and Taejin Red were paired-end sequenced using the Illumina HiSeq 2000 system. The raw data in this study can be available in NCBI SRA database with accession numbers of SRR3406...

  8. Dicty_cDB: Contig-U11872-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available igna unguiculata cDNA 5', mRNA seq... 46 3.4 1 ( FF392686 ) MOOCW41TF MOO Vigna unguiculata cDNA 5', mRNA se...q... 46 3.4 1 ( FF388806 ) MOOC456TF MOO Vigna unguiculata cDNA 5', mRNA seq... 46 3.4 1 ( AC174299 ) Medica

  9. The phosphorus and nitrogen nutrition of bambara groundnut (Vigna subterranea (L.) Verdc.) in Botswana soils : An exploratory study

    NARCIS (Netherlands)

    Ramolemana, G.M.

    1999-01-01

    Bambara groundnut ( Vigna subterranea (L.) Verdec.) is a legume crop grown especially by small farmers mainly in semi-arid parts of Africa both in mixed cultivation and pure stands. It is considered as a hardy crop because of its drought tolerance, resistance to pests and diseases and ability to yie

  10. Polyamine levels as related to growth, differentiation and senescence in protoplast-derived cultures of Vigna aconitifolia and Avena sativa

    Science.gov (United States)

    Kaur Sawhney, R.; Shekhawat, N. S.; Galston, A. W.

    1985-01-01

    We have previously reported that aseptically cultured mesophyll protoplasts of Vigna divide rapidly and regenerate into complete plants, while mesophyll protoplasts of Avena divide only sporadically and senesce rapidly after isolation. We measured polyamine titers in such cultures of Vigna and Avena, to study possible correlations between polyamines and cellular behavior. We also deliberately altered polyamine titer by the use of selective inhibitors of polyamine biosynthesis, noting the effects on internal polyamine titer, cell division activity and regenerative events. In Vigna cultures, levels of free and bound putrescine and spermidine increased dramatically as cell division and differentiation progressed. The increase in bound polyamines was largest in embryoid-forming callus tissue while free polyamine titer was highest in root-forming callus. In Avena cultures, the levels of total polyamines decreased as the protoplast senesced. The presence of the inhibitors alpha-difluoromethyl-arginine (specific inhibitor of arginine decarboxylase), alpha-difluoromethylornithine (specific inhibitor of ornithine decarboxylase) and dicyclohexylamine (inhibitor of spermidine synthase) reduced cell division and organogenesis in Vigna cultures. Addition of low concentration of polyamines to such cultures containing inhibitors or removal of inhibitors from the culture medium restored the progress of growth and differentiation with concomitant increase in polyamine levels.

  11. Separation and Characterization of Two Endopeptidases from Cotyledons of Germinating Vigna mungo Seeds.

    Science.gov (United States)

    Mitsuhashi, W; Koshiba, T; Minamikawa, T

    1986-03-01

    Two major endopeptidases were present in cotyledons of germinating Vigna mungo seeds, as detected by the zymogram after polyacrylamide gel electrophoresis. They were not detectable in cotyledons of dry seeds, but their intensities on the zymogram increased during germination. During incubation of detached cotyledons, however, the activities showed only a slight increase for 5 days. These two endopeptidases could be separated by Sephacryl S-200 column chromatography. One of them was found to be a serine-endopeptidase as judged by phenylmethylsulfonylfluoride and diisopropyl fluorophosphate inhibition. The other was a sulfhydryl-endopeptidase because of its dependency on 2-mercaptoethanol and inhibition by leupeptin, chymostatin, and antipain. Analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis indicatd that the two endopeptidases digested the Vigna mungo seed globulin subunits at different rates. The serine enzyme digested the 56 kilodalton subunit at first, but the sulfhydryl enzyme digested the 54 kilodalton peptide more efficiently than the 56 kilodalton peptide. The pattern of digestion of globulin by the combination of the serine- and sulfhydryl-endopeptidases was similar to that using crude enzyme extracts.

  12. Separation and Characterization of Two Endopeptidases from Cotyledons of Germinating Vigna mungo Seeds 1

    Science.gov (United States)

    Mitsuhashi, Wataru; Koshiba, Tomokazu; Minamikawa, Takao

    1986-01-01

    Two major endopeptidases were present in cotyledons of germinating Vigna mungo seeds, as detected by the zymogram after polyacrylamide gel electrophoresis. They were not detectable in cotyledons of dry seeds, but their intensities on the zymogram increased during germination. During incubation of detached cotyledons, however, the activities showed only a slight increase for 5 days. These two endopeptidases could be separated by Sephacryl S-200 column chromatography. One of them was found to be a serine-endopeptidase as judged by phenylmethylsulfonylfluoride and diisopropyl fluorophosphate inhibition. The other was a sulfhydryl-endopeptidase because of its dependency on 2-mercaptoethanol and inhibition by leupeptin, chymostatin, and antipain. Analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis indicatd that the two endopeptidases digested the Vigna mungo seed globulin subunits at different rates. The serine enzyme digested the 56 kilodalton subunit at first, but the sulfhydryl enzyme digested the 54 kilodalton peptide more efficiently than the 56 kilodalton peptide. The pattern of digestion of globulin by the combination of the serine- and sulfhydryl-endopeptidases was similar to that using crude enzyme extracts. Images Fig. 2 Fig. 3 Fig. 6 Fig. 7 PMID:16664675

  13. Genetic diversity as assessed by ISSR markers in Blackgram (Vigna mungo (L. Hepper

    Directory of Open Access Journals (Sweden)

    Nadarajan N

    2009-12-01

    Full Text Available An investigation was carried out on a collection of 23 blackgram genotypes involving 16 releasedvarieties, six pre release cultures and one wild species Vigna mungo var. silvestris to study the genetic diversityusing twelve ISSR primers. The number of alleles produced by different ISSR primers ranged from eight to 17with an average of 11.5 per primer and the level of polymorphism was found to be 82.05 percent. Similaritymeasures and clustering analyses were made using ISSR data. The resulting dendrogram distributed the 23blackgram genotypes into five main clusters. The highest genetic similarity coefficient was measured betweengenotypes CBG 671 and CBG 632. The results of PCoA were comparable to that of grouping based on UPGMAand 23 genotypes were grouped into four groups. Genotype Vigna mungo var. silvestris was placed separatelyfrom rest of the genotypes in both the analyses. Grouping of varieties using ISSR markers did not show anyrelevance to their pedigree. All the pre release cultures in one group revealed that only a portion of geneticvariation has been exploited. The results revealed that, genetic diversity is low among the varieties releasedfrom the respective institute and hence genotypes were grouped according to the research institutes from whichthey released. It suggests that the research institutes have to enlarge the genetic base for variety development.

  14. Comparative Studies of Nitrogen Fixing Potential of Desmodium ramississimon and Vigna unquiculata for Soil Fertility Management

    Directory of Open Access Journals (Sweden)

    Ngwu, OE.

    2005-01-01

    Full Text Available The occurrence of large numbers of legume species in the tropics with potentials for nitrogen fixation could be exploited to supply nitrogen, if they can be integrated into the farming system. The N2 – fixing potential of a native herbaceous leguminous species namely, Desmodium ramississimon (Dm and grain legume, Vigna unquiculata (Cp were studied in the green house and field, on three types of soil. In both situations, nodulation was influenced by the soil type. Nsukka soil, which had sandy texture, highest level of available phosphorus among the soils investigated in the study and moderate level of other plant nutrients (Mg and K enhanced nodulation, which supported N-fixation. Soil type also influenced the quantity of N accumulated by each species, but had no effect on nitrogen concentration in the different plant parts. Desmodium ramississimon had higher nodule weight and accumulated more nitrogen and fixed more N2 than Vigna unquiculata in the three soils. The mean nodule dry weights were in the ranges of 61.6- 239.2 mg/plant for Dm in the three soils as compared to the range 3.2-31.4 mg/plant for Cp. Symbiotic dependence of DM varied with soil type ranging from 63.62% in Adani soil to 88% in Nkpologu soil, whereas Cp had the least symbiotic dependence value. These trends were confirmed in the field thereby indicating that Desmodium ramississimon had greater N2- potential than the cultivated legume studied.

  15. Morphological responses of pulse (Vigna spp. crops to soil water deficit

    Directory of Open Access Journals (Sweden)

    Baroowa Bhaswatee

    2012-01-01

    Full Text Available The present experiment was conducted with two common pulse crops namely black gram (Vigna mungo L. and green gram (Vigna radiata L. with the objective to study the morpho-physiological changes that took place in response to low moisture stress. Parameters such as plant height, leaf number, leaf area and pod number were studied under moisture stress condition as well as subsequent recovery stages. At harvest, yields of these two crops were recorded and various yield indexes like drought susceptibility index, drought tolerance index, mean and productivity rate were calculated. The study revealed that moisture stress has a significant impact on all these parameters in both crops. The effect was more significant in green gram compared to black gram. From the findings it is observed that moisture stress during flowering stage is detrimental for yield of the pulse crops and re-watering does not have a significant impact on yield improvement. Black gram variety T9 and green gram variety Pratap were identified as drought-tolerant varieties.

  16. Stable transformation of moth bean Vigna aconitifolia via direct gene transfer.

    Science.gov (United States)

    Köhler, F; Golz, C; Eapen, S; Kohn, H; Schieder, O

    1987-07-01

    Direct gene transfer proved to be an efficient transformation method for Vigna aconitifolia, a member of the legume family. Kanamycin resistant calli and plants were regenerated from heat shocked protoplasts treated with PEG and plasmid DNA containing the coding region for aminoglycoside phosphotransferase gene (NPT II). The plant cultivar used was an important factor in attaining higher transformation frequencies. Transformation was confirmed by Southern blot analysis using a non-radioactive detection system. Attempts to transform mesophyll and suspension cultured cells by this method were unsuccessful. Protoplasts electroporated with the plasmid pCAP212, which codes for chloramphenicol acetyltransferase, exhibited transient expression of this gene two days after treatment while electroporated cells did not show this enzyme activity. It is therefore assumed that the DNA uptake is prevented by the cell wall.

  17. Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L. Seeds and Their Divergence

    Directory of Open Access Journals (Sweden)

    Neha Gupta

    2016-01-01

    Full Text Available In the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of photochemical composition was correlated with seed storage proteins like albumin and globulin. Qualitative identification of total seed storage protein abundance across two related moth bean accessions using two-dimensional gel electrophoresis (2D-GE was performed. Over 20 individual protein fractions were distributed over the gel as a series of spots in two moth bean accessions. Seed proteome accumulated spots of high intensity over a broad range of pI values of 3–10 in a molecular weight range of 11–170 kDa. In both seed accessions maximum protein spots are seen in the pI range of 6–8.

  18. STUDIES ON THE INFLUENCE OF PHYSICAL MUTAGEN (GAMMA IRRADIATION IN BLACK GRAM (VIGNA MUNGO (L. HEPPER

    Directory of Open Access Journals (Sweden)

    *M. DHASARATHAN, 1N. MEENAKSHIGANESAN AND 2S. GEETHA

    2014-10-01

    Full Text Available Black gram (Vigna mungo (L. Hepper variety TNAU (Bg CO 6 was exposed to varying doses of gamma rays to study their effect on various characters like seed germination and seedling survival, plant height at 30th day and at maturity, pollen and seed fertility. In M1 generation the results revealed that there was more reduction at higher doses compared to lower doses for all the characters studied. The higher doses of gamma rays lead to lethal injury and sterility. Effects on these traits have been attributed to the physiological disturbance or chromosomal damage of the cells caused by the gamma rays. The results of the present study clearly indicate that different doses of gamma rays can be effectively utilized to create variability for various quantitative traits in black gram.

  19. Imunohistochemical Localization of alpha-Amylase in Cotyledons of Vigna mungo Seedlings.

    Science.gov (United States)

    Tomura, H; Koshiba, T; Minamikawa, T

    1985-12-01

    We studied the localization of alpha-amylase with indirect fluorescence microscopy in transversely sectioned cotyledons of Vigna mungo seedlings. Tissue sections were fixed in periodate-lysine-paraformaldehyde and treated with anti-alpha-amylase immunoglobulin G followed by fluorescein isothiocyanate labeled goat anti-rabbit immunoglobulin G. alpha-Amylase appeared in the cells farthest from vascular bundles on the second day of growth and appeared gradually closer to the vascular bundles as growth progressed. The pattern of alpha-amylase appearance was similar in detached cotyledons, indicating that attachment of the embryonic axis has no effect on this pattern. However, in attached cotyledons, alpha-amylase disappeared from the regions where starch grains had been digested, but in detached cotyledons there was no disappearance of alpha-amylase, and digestion was slower than in intact cotyledons.

  20. Imunohistochemical Localization of α-Amylase in Cotyledons of Vigna mungo Seedlings 1

    Science.gov (United States)

    Tomura, Hideaki; Koshiba, Tomokazu; Minamikawa, Takao

    1985-01-01

    We studied the localization of α-amylase with indirect fluorescence microscopy in transversely sectioned cotyledons of Vigna mungo seedlings. Tissue sections were fixed in periodate-lysine-paraformaldehyde and treated with anti-α-amylase immunoglobulin G followed by fluorescein isothiocyanate labeled goat anti-rabbit immunoglobulin G. α-Amylase appeared in the cells farthest from vascular bundles on the second day of growth and appeared gradually closer to the vascular bundles as growth progressed. The pattern of α-amylase appearance was similar in detached cotyledons, indicating that attachment of the embryonic axis has no effect on this pattern. However, in attached cotyledons, α-amylase disappeared from the regions where starch grains had been digested, but in detached cotyledons there was no disappearance of α-amylase, and digestion was slower than in intact cotyledons. Images Fig. 1 Fig. 2 Fig. 3 PMID:16664548

  1. AgNO3 boosted high-frequency shoot regeneration in Vigna mungo (L.) Hepper.

    Science.gov (United States)

    Mookkan, Muruganantham; Andy, Ganapathi

    2014-01-01

    In order to further increase shoot regeneration frequency of Vigna mungo (L.) Hepper., the effects of AgNO3 on this process was investigated in this study. The shoot tip and cotyledonary node explants were cultured on MS salts B5 Vitamins medium containing BA+TDZ+Ads+AgNO3 for multiple shoot induction. AgNO3 influenced the shoot bud formation and their subsequent proliferation. The best medium composition for multiple shoot induction was BA, TDZ combination with Ads and AgNO3 in MSB5 medium. Maximum 39 shoots in cotyledonary node and 22 shoots in shoot tip were obtained per explants after 4 - 6 wk. of culture. Elongation and rooting were performed in GA3 (0.6mg/l) and IBA (0.4mg/L) containing media respectively. The in vitro raised plantlets were acclimatized in green house and successfully transplanted to the field with a survival rate of 78%.

  2. BIO-ACCUMULATION AND RELEASE OF MERCURY IN VIGNA MUNGO (L. HEPPER SEEDLINGS

    Directory of Open Access Journals (Sweden)

    Hussain. K

    2010-09-01

    Full Text Available Effect of mercury on the seedling of Vigna mungo seedlings was studied by culturing the seedlings in Hoagland medium artificially contaminated with 5 and 10mM Mercuric Chloride. Histochemical localization of the mercury in shoot and root tissues was done by staining with dithizone and quantitative analyses of mercury content accumulated in root, stem and leaf tissues were done using mercury analyser. Localization of mercury was observed as coloured masses in the cells of root and stem. Stem tissues of seedlings showed anatomical modification in the epidermal cells as trichomes. Patterns of bioaccumulation of mercury was root> stem> leaves revealing feeble translocation to the shoot system. A comparison of residual mercury content retained in the growth medium after sample harvesting and quantity accumulated in the plant body reveals that some quantity of mercury is lost presumably through the trichomes developed on the stem and/ or through stomata of the leaves.

  3. Molecular dynamic and docking interaction study of Heterodera glycines serine proteinase with Vigna mungo proteinase inhibitor.

    Science.gov (United States)

    Prasad, C V S Siva; Gupta, Saurabh; Gaponenko, Alex; Tiwari, Murlidhar

    2013-08-01

    Many plants do produce various defense proteins like proteinase inhibitors (PIs) to protect them against various pests. PIs function as pseudosubstrates of digestive proteinase, which inhibits proteolysis in pests and leads to amino acid deficiency-based mortality. This work reports the structural interaction studies of serine proteinase of Heterodera glycines (SPHG) with Vigna mungo proteinase inhibitor (VMPI). 3D protein structure modeling, validation of SPHG and VMPI, and their putative protein-protein binding sites were predicted. Protein-protein docking followed by molecular dynamic simulation was performed to find the reliable confirmation of SPHG-VMPI complex. Trajectory analysis of each successive conformation concludes better interaction of first loop in comparison with second loop. Lysine residues of first loop were actively participating in complex formation. Overall, this study discloses the structural aspects and interaction mechanisms of VMPI with SPHG, and it would be helpful in the development of pest-resistant genetically modified crops.

  4. Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence.

    Science.gov (United States)

    Gupta, Neha; Shrivastava, Nidhi; Singh, Pramod Kumar; Bhagyawant, Sameer S

    2016-01-01

    In the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia) seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of photochemical composition was correlated with seed storage proteins like albumin and globulin. Qualitative identification of total seed storage protein abundance across two related moth bean accessions using two-dimensional gel electrophoresis (2D-GE) was performed. Over 20 individual protein fractions were distributed over the gel as a series of spots in two moth bean accessions. Seed proteome accumulated spots of high intensity over a broad range of pI values of 3-10 in a molecular weight range of 11-170 kDa. In both seed accessions maximum protein spots are seen in the pI range of 6-8. PMID:27239343

  5. Vigna radiata as a New Source for Biotransformation of Hydroquinone to Arbutin

    Directory of Open Access Journals (Sweden)

    Zahra Tofighi, Mohsen Amini, Mahzad Shirzadi, Hamideh Mirhabibi, Negar Ghazi Saeedi, Narguess Yassa

    2016-06-01

    Full Text Available Background: The suspension culture of Vigna radiata was selected for biotransformation of hydroquinone to its β-D-glucoside form (arbutin as an important therapeutic and cosmetic compound. Methods: The biotransformation efficiency of a Vigna radiata cell culture in addition to different concentrations of hydroquinone (6-20 mg/100 ml was investigated after 24 hours in comparison to an Echinacea purpurea cell culture and attempts were made to increase the efficacy of the process by adding elicitors. Results: Arbutin was accumulated in cells and found in the media only in insignificant amounts. The arbutin content of the biomass extracts of V. radiata and E. purpurea was different, ranging from 0.78 to 1.89% and 2.00 to 3.55% of dry weight, respectively. V. radiata demonstrated a bioconversion efficiency of 55.82% after adding 8 mg/100 ml precursor, which was comparable with result of 69.53% for E. purpurea cells after adding 10 mg/100 ml hydroquinone (P>0.05. In both cultures, adding hydroquinone in two portions with a 24-hour interval increased the biotransformation efficiency. Different concentrations of methyl jasmonate (25, 50, and 100 µM and chitosan (50 and 100 µg/ml as elicitors increased the bio-efficiency percentage of the V. radiata culture in comparison with the flask containing only hydroquinone. Conclusion: This is the first report of the biotransformation possibility of V. radiata cultures. It was observed the bioconversion capacity increased by adding hydroquinone in two portions, which was comparable to adding an elicitor.

  6. The complete mitochondrial genome of mungbean Vigna radiata var. radiata NM92 and a phylogenetic analysis of crops in angiosperms.

    Science.gov (United States)

    Lin, Ching-Ping; Lo, Hsiao-Feng; Chen, Chien-Yu; Chen, Long-Fang Oliver

    2016-09-01

    The entire mitogenome of the Vigna radiata var. radiata NM92 was identified as a circular molecule of 401 262 bp length (DDBJ accession number: AP014716). The contents of A, T, C, and G in the NM92 mitogenome were found to be 27.48%, 27.41%, 22.63%, and 22.48%, respectively. The NM92 mitogenome encoded 3 rRNAs, 16 tRNAs and 33 proteins. Eight protein-coding genes (nad1, nad2, nad4, nad5, nad7, rps3, and rps10) centain introns. Among them, three (nad1, nad2, and nad5) are trans-spliced genes. A phylogenetic tree was reconstructed using the 21 protein-coding genes of 16 crops. A species of gymnosperms, Cycas, was selected as the outgroup. This complete mitogenome sequence provides useful information to understand the cultivation of Vigna radiata and other crops. PMID:26469726

  7. Differentiation of aecidiospore-and uredospore-derived infection structures on cowpea leaves and on artificial surfaces by Uromyces vignae

    OpenAIRE

    Stark-Urnau, Martina; Mendgen, Kurt

    1993-01-01

    Aecidiospores and uredospores are the two dikaryotic spore fonus of the cowpea rust fungus Uromyees vignae. After germination they can be induced to develop a series of infection structures including appressoria, infection hyphae, and haustorial mother cells. Haustoria are then fonned within host cells. The differentiation of infection structures was compared on polystyrene membranes with defined topographies, on-scratched polyethylene membranes, and in ·planta. On polystyrene membranes with ...

  8. DEVELOPMENTAL CHANGES IN THE GERMINATION, GROWTH AND CHLOROPHYLLASE ACTIVITY OF VIGNA MUNGO L. USING SEAWEED EXTRACT OF ULVA RETICULATA FORSSKAL.

    Directory of Open Access Journals (Sweden)

    Ganapathy Selvam G.

    2013-01-01

    Full Text Available The effect of seaweed extract prepared from Ulva reticulata on seed germination, seedling growth and chlorophyllase activity of Vigna mungo L. was studied. 100% germination was recorded in the seeds treated with lower concentration of seaweed extract. The V. mungo seeds soaked with lower concentrations of the seaweed extracts showed higher rates of germination, while the higher concentrations of the extracts inhibited the germination.

  9. Development of Gene-Based SSR Markers in Rice Bean (Vigna umbellata L.) Based on Transcriptome Data

    OpenAIRE

    Honglin Chen; Xin Chen; Jing Tian; Yong Yang; Zhenxing Liu; Xiyu Hao; Lixia Wang; Suhua Wang; Jie Liang; Liya Zhang; Fengxiang Yin; Xuzhen Cheng

    2016-01-01

    Rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi) is a warm season annual legume mainly grown in East Asia. Only scarce genomic resources are currently available for this legume crop species and no simple sequence repeat (SSR) markers have been specifically developed for rice bean yet. In this study, approximately 26 million high quality cDNA sequence reads were obtained from rice bean using Illumina paired-end sequencing technology and assembled into 71,929 unigenes with an average length o...

  10. Probabilistic point source inversion of strong-motion data in 3-D media using pattern recognition: A case study for the 2008 Mw 5.4 Chino Hills earthquake

    Science.gov (United States)

    Käufl, Paul; Valentine, Andrew P.; Trampert, Jeannot

    2016-08-01

    Despite the ever increasing availability of computational power, real-time source inversions based on physical modeling of wave propagation in realistic media remain challenging. We investigate how a nonlinear Bayesian approach based on pattern recognition and synthetic 3-D Green's functions can be used to rapidly invert strong-motion data for point source parameters by means of a case study for a fault system in the Los Angeles Basin. The probabilistic inverse mapping is represented in compact form by a neural network which yields probability distributions over source parameters. It can therefore be evaluated rapidly and with very moderate CPU and memory requirements. We present a simulated real-time inversion of data for the 2008 Mw 5.4 Chino Hills event. Initial estimates of epicentral location and magnitude are available ˜14 s after origin time. The estimate can be refined as more data arrive: by ˜40 s, fault strike and source depth can also be determined with relatively high certainty.

  11. Purification, properties and alternate substrate specificities of arginase from two different sources: Vigna catjang cotyledon and buffalo liver

    Directory of Open Access Journals (Sweden)

    2005-08-01

    Full Text Available Arginase was purified from Vigna catjang cotyledons and buffalo liver by chromatographic separations using Bio-Gel P-150, DEAE-cellulose and arginine AH Sepharose 4B affinity columns. The native molecular weight of an enzyme estimated on Bio-Gel P-300 column for Vigna catjang was 210 kDa and 120 kDa of buffalo liver, while SDS-PAGE showed a single band of molecular weight 52 kDa for cotyledon and 43 kDa for buffalo liver arginase. The kinetic properties determined for the purified cotyledon and liver arginase showed an optimum pH of 10.0 and pH 9.2 respectively. Optimal cofactor Mn++ ion concentration was found to be 0.6 mM for cotyledon and 2 mM for liver arginase. The Michaelis-Menten constant for cotyledon arginase and hepatic arginase were found to be 42 mM and 2 mM respectively. The activity of guanidino compounds as alternate substrates for Vigna catjang cotyledon and buffalo liver arginase is critically dependent on the length of the amino acid side chain and the number of carbon atoms. In addition to L-arginine cotyledon arginase showed substrate specificity towards agmatine and L-canavanine, whereas the liver arginase showed substrate specificity towards only L-canavanine.

  12. Genotypic Variation for N2-FIXATION in Voandzou (vigna Subterranea) Under P Deficiency and P Sufficiency

    Science.gov (United States)

    Andry, A.; Mahamadou, M.; Lilia, R.; Laurie, A.; Hélène, V.; Dominique, M.; Christian, M.; Jean-Jacques, D.

    2011-12-01

    Genetic variation associated with N2 fixation exists in numerous legume species (Graham, 2004). High symbiotic N2 fixation under P deficiency is related closely to nodulation which was used in legume selection for N2 fixation (Herridge and Rose, 2000). Until now, study of genetic potential of neglected crops like Vigna subterranea (bambara groundnut or voandzou) is often limited while its agronomic properties is interesting for the farmers of Africa. In order to assess the genotypic variation of voandzou for tolerance to phosphorus deficiency, a physiological approach of cultivar selection was performed with 54 cultivars from Madagascar, Niger and Mali in hydroponic culture under P deficiency and P sufficiency and inoculated with the reference strain of Bradyrhizobium sp. Vigna CB756. The results of nodulation and plant biomass, which are closely related, showed a large dispersion between cultivars (0.05-0.43 g nodule dry weight per plant and 0.50-5.51 g shoot dry weight per plant). The cultivars which presented the maximum growth during the experiment presented a high efficiency in use of the rhizobial symbiosis calculated as the slope of plant biomass regression as a function of nodulation. A large increase in nodulated-root O2 consumption under P deficiency was observed for the two most tolerant cultivars. The microscopic analysis with in situ RT-PCR of the nodule sections showed an increase of a phytase gene expression with tolerance of cultivars to P deficiency. From two most contrasting cultivars, an isotopic exchange method 32P was carried out on rhizosphere soil in rhizotron culture in order to assess the direct effect induced by the roots in terms of phosphorus mobilization. The rhizospheric effect was observed under P deficiency marked by a strong re-supplying capacity of soil solution in the diffusive phosphate ion between solid phase and soil solution leading to great phosphorus nutrition. These results highlight the genotypic variability among voandzou

  13. Analysis of simple sequence repeats in rice bean (Vigna umbellata using an SSR-enriched library

    Directory of Open Access Journals (Sweden)

    Lixia Wang

    2016-02-01

    Full Text Available Rice bean (Vigna umbellata Thunb., a warm-season annual legume, is grown in Asia mainly for dried grain or fodder and plays an important role in human and animal nutrition because the grains are rich in protein and some essential fatty acids and minerals. With the aim of expediting the genetic improvement of rice bean, we initiated a project to develop genomic resources and tools for molecular breeding in this little-known but important crop. Here we report the construction of an SSR-enriched genomic library from DNA extracted from pooled young leaf tissues of 22 rice bean genotypes and developing SSR markers. In 433,562 reads generated by a Roche 454 GS-FLX sequencer, we identified 261,458 SSRs, of which 48.8% were of compound form. Dinucleotide repeats were predominant with an absolute proportion of 81.6%, followed by trinucleotides (17.8%. Other types together accounted for 0.6%. The motif AC/GT accounted for 77.7% of the total, followed by AAG/CTT (14.3%, and all others accounted for 12.0%. Among the flanking sequences, 2928 matched putative genes or gene models in the protein database of Arabidopsis thaliana, corresponding with 608 non-redundant Gene Ontology terms. Of these sequences, 11.2% were involved in cellular components, 24.2% were involved molecular functions, and 64.6% were associated with biological processes. Based on homolog analysis, 1595 flanking sequences were similar to mung bean and 500 to common bean genomic sequences. Comparative mapping was conducted using 350 sequences homologous to both mung bean and common bean sequences. Finally, a set of primer pairs were designed, and a validation test showed that 58 of 220 new primers can be used in rice bean and 53 can be transferred to mung bean. However, only 11 were polymorphic when tested on 32 rice bean varieties. We propose that this study lays the groundwork for developing novel SSR markers and will enhance the mapping of qualitative and quantitative traits and marker

  14. Analysis of simple sequence repeats in rice bean (Vigna umbellata) using an SSR-enriched library

    Institute of Scientific and Technical Information of China (English)

    Lixia Wang; Kyung Do Kim; Dongying Gao; Honglin Chen; Suhua Wang; SukHa Lee; Scott A. Jackson; Xuzhen Cheng

    2016-01-01

    Rice bean (Vigna umbellata Thunb.), a warm-season annual legume, is grown in Asia mainly for dried grain or fodder and plays an important role in human and animal nutrition because the grains are rich in protein and some essential fatty acids and minerals. With the aim of expediting the genetic improvement of rice bean, we initiated a project to develop genomic resources and tools for molecular breeding in this little-known but important crop. Here we report the construction of an SSR-enriched genomic library from DNA extracted from pooled young leaf tissues of 22 rice bean genotypes and developing SSR markers. In 433,562 reads generated by a Roche 454 GS-FLX sequencer, we identified 261,458 SSRs, of which 48.8% were of compound form. Dinucleotide repeats were predominant with an absolute proportion of 81.6%, followed by trinucleotides (17.8%). Other types together accounted for 0.6%. The motif AC/GT accounted for 77.7%of the total, followed by AAG/CTT (14.3%), and all others accounted for 12.0%. Among the flanking sequences, 2928 matched putative genes or gene models in the protein database of Arabidopsis thaliana, corresponding with 608 non-redundant Gene Ontology terms. Of these sequences, 11.2%were involved in cellular components, 24.2%were involved molecular functions, and 64.6%were associated with biological processes. Based on homolog analysis, 1595 flanking sequences were similar to mung bean and 500 to common bean genomic sequences. Comparative mapping was conducted using 350 sequences homologous to both mung bean and common bean sequences. Finally, a set of primer pairs were designed, and a validation test showed that 58 of 220 new primers can be used in rice bean and 53 can be transferred to mung bean. However, only 11 were polymorphic when tested on 32 rice bean varieties. We propose that this study lays the groundwork for developing novel SSR markers and will enhance the mapping of qualitative and quantitative traits and marker-assisted selection in

  15. Feasibility Study of Economics and Performance of Solar Photovoltaics at the Chino Mine in Silver City, New Mexico. A Study Prepared in Partnership with the Environmental Protection Agency for the RE-Powering America's Land Initiative: Siting Renewable Energy on Potentially Contaminated Land and Mine Sites

    Energy Technology Data Exchange (ETDEWEB)

    Kiatreungwattana, K.; Geiger, J.; Healey, V.; Mosey, G.

    2013-04-01

    The U.S. Environmental Protection Agency (EPA), in accordance with the RE-Powering America's Land initiative, selected the Chino Mine site in Silver City, New Mexico, for a feasibility study of renewable energy production. The National Renewable Energy Laboratory (NREL) provided technical assistance for this project. The purpose of this report is to assess the site for a possible photovoltaic (PV) system installation and estimate the cost, performance, and site impacts of different PV options. In addition, the report recommends financing options that could assist in the implementation of a PV system at the site.

  16. 《班华字典-福安方言》音系初探%Preliminary Research on the Phonology of Diccionario Espanol-Chino , Dialecto de Fu-an 《班华字典-福安方言》

    Institute of Scientific and Technical Information of China (English)

    秋谷裕幸

    2012-01-01

    The paper describes the phonological system of Diccionario Espa ol-Chino, Dialecto de Fu-an, a dictionary published between 1941 and 1943 recording dialect of Fu'an in Fujian province. It also describes the phonological changes during the period of more than 100 years and lists the homophony syllabary.%本文分析《班华字典-福安方言》(1941—1943)的音系,并考察了近一两百年来福安方音的演变,同时还整理出同音字汇。

  17. De novo transcriptome assembly of two Vigna angularis varieties collected from Korea.

    Science.gov (United States)

    Jo, Yeonhwa; Lian, Sen; Cho, Jin Kyong; Choi, Hoseong; Kim, Sang-Min; Kim, Sun-Lim; Lee, Bong Choon; Cho, Won Kyong

    2016-06-01

    The adzuki bean (Vigna angularis), a member of the family Fabaceae, is widely grown in Asia, from East Asia to the Himalayas. The adzuki bean is known as an ingredient that adds sweetness to diverse desserts made in Eastern Asian countries. Libraries prepared from two V. angularis varieties referred to as Taejin Black and Taejin Red were paired-end sequenced using the Illumina HiSeq 2000 system. The raw data in this study can be available in NCBI SRA database with accession numbers of SRR3406660 and SRR3406553. After de novo transcriptome assembly using Trinity, we obtained 324,219 and 280,056 transcripts from Taejin Black and Taejin Red, respectively. We predicted a total of 238,321 proteins and 179,519 proteins for Taejin Black and Taejin Red, respectively, by the TransDecoder program. We carried out BLASTP on the predicted proteins against the Swiss-Prot protein sequence database to predict the putative functions of identified proteins. Taken together, we provide transcriptomes of two adzuki bean varieties by RNA-Seq, which might be usefully applied to generate molecular markers. PMID:27257605

  18. Biofortification of mungbean (Vigna radiata) as a whole food to enhance human health.

    Science.gov (United States)

    Nair, Ramakrishnan M; Yang, Ray-Yu; Easdown, Warwick J; Thavarajah, Dil; Thavarajah, Pushparajah; Hughes, Jacqueline d'A; Keatinge, J D H Dyno

    2013-06-01

    Mungbean (Vigna radiata (L.) R. Wilczek var. radiata) is one of the most important pulse crops grown in South, East and Southeast Asia. It provides significant amounts of protein (240 g kg(-1)) and carbohydrate (630 g kg(-1)) and a range of micronutrients in diets. Mungbean protein and carbohydrate are easily digestible and create less flatulence than proteins derived from other legumes. In addition, mungbean is lower in phytic acid (72% of total phosphorus content) than pigeonpea (Cajanus cajan L. Millsp.), soybean (Glycine max L.) and cereals; phytic acid is commonly found in cereal and legume crops and has a negative impact on iron and zinc bioavailability in plant-based diets. Owing to its palatable taste and nutritional quality, mungbean has been used as an iron-rich whole food source for baby food. The wide genetic variability of mineral concentrations (e.g. 0.03-0.06 g Fe kg(-1), 0.02-0.04 g Zn kg(-1)) in mungbean indicates possibilities to improve its micronutrient content through biofortification. Therefore biofortification of existing mungbean varieties has great potential for enhancing the nutritional quality of diets in South and Southeast Asia, where protein and micronutrient malnutrition are among the highest in the world. This review paper discusses the importance of mungbean in agricultural production and traditional diets and the potential of enhancing the nutritional quality of mungbean through breeding and other means, including agronomic practices. PMID:23426879

  19. Ameliorating Effects of Iron and Zinc on Vigna mungo L. Treated with Tannery Effluent.

    Science.gov (United States)

    Srivastava, Shefali; Mishra, Kumkum; Tandon, Pramod Kumar

    2014-01-01

    Different dilutions, that is, 25, 50, 75, and 100%, of tannery effluent (TE) were chosen for the present study to assess the phytotoxic effects on Vigna mungo L. For amelioration purposes, different levels and combinations of iron and zinc were supplied to the plants along with 50% TE that is chosen on the basis of prior test under Petri dish culture. Cytotoxic and biochemical analysis and plant tolerance index (PTI) of plant were observed. Mitotic index deceased with increase in effluent concentration whereas abnormality % was increased. The pigments (chlorophyll a, total, and carotenoids) were decreased with increasing treatment levels of TE at both growth stages. However, carotenoid content increased significantly at all dilution levels of TE after first growth stage. Chlorophyll b was increased significantly after 35 days of growth but decreased after 70 days. The protein contents were also significantly decreased with increase in all TE treatments and increased significantly in zinc recovery treatments. Activities of catalase and peroxidase enzymes were significantly affected and increased significantly with effluent treatments. PTI showed an enhanced tolerance capacity of plant with treatment of iron and zinc. A negative correlation was found (r = -0.97) between plant height and different dilutions of effluent whereas it was positively correlated (r = 0.95) with iron and zinc treatments. The study represents the ameliorative effect of iron and zinc for phytotoxic damage in V. mungo caused by tannery effluent.

  20. Properties of a cyclic 3'5'-nucleotide phosphodiesterase from Vigna mungo.

    Science.gov (United States)

    Lee, C H; Abidin, U Z

    1989-10-01

    Cyclic AMP phosphodiesterase (PDE) partially purified from roots of Vigna mungo exhibited optimum activity at pH 5.5 to 6.0 and maximum enzyme activity at 50 degrees C. Levels of PDE activity in roots remained relatively constant from the first to the eleventh day after germination; on the twelfth day there was a 400% increase in PDE activity. The enzyme was stable for at least 48 hours at 28 degrees C, retaining 92% of its original activity. Plant growth hormones including gibberellic acid, indoleacetic acid and kinetin at 1.0 and 10.0 microM concentrations did not have any significant effect on enzyme activity. Nucleotides tested including cyclic 2'3' AMP, cyclic 2'3' GMP completely abolished enzyme activity at 1.0mM while cyclic 3'5' GMP, cyclic 3'5' GMP, 2'deoxy 5' ATP, 2'deoxy 5'GTP and 5'ADP were also inhibitory to the enzyme. The enzyme was stimulated by Mg2+, Fe2+ and NH4+ while Cu2+ and Fe3+ were inhibitory. Theophylline, caffeine, phosphate, pyrophosphate and EDTA were inhibitory to the enzyme.

  1. Effect of Arsenic on Nodulation and Nitrogen Fixation of Blackgram (Vigna mungo).

    Science.gov (United States)

    Mandal, Santi M; Gouri, Samiran S; De, Debasis; Das, Bidus K; Mondal, Keshab C; Pati, Bikas R

    2011-01-01

    Rhizobium-legume symbiotic interaction is an efficient model system for soil remediation and reclamation. We earlier isolated an arsenic (As) (2.8 mM arsenate) tolerant and symbiotically effective Rhizobium strain, VMA301 from Vigna mungo and in this study we further characterized its efficacy for arsenic removal from the soil and its nitrogen fixation capacity. Although nodule formation is delayed in plants with As-treated composite when the inoculum was prepared without arsenic in culture medium, whereas it attains the significant number of nodules compare to plant grown in As-free soil when the inoculum was prepared with arsenic supplemented medium. Arsenic accumulation was higher in roots than root nodules. Nitrogenase activity is reduced to almost 2 fold in plants with As-treated soil but not abolished. These results suggest that this strain, VMA301, has been able to establish an effective symbiotic interaction in V. mungo in As-contaminated soil and can perform dual role of arsenic bioremediation as well as soil nitrogen improvement.

  2. Proteomic analysis of salicylic acid induced resistance to Mungbean Yellow Mosaic India Virus in Vigna mungo.

    Science.gov (United States)

    Kundu, Subrata; Chakraborty, Dipjyoti; Pal, Amita

    2011-03-01

    The role of salicylic acid (SA) in inducing resistance to MYMIV infection in Vigna mungo has been elucidated by proteomics. Twenty-nine proteins identified by MALDI-TOF/TOF, predicted to be involved in stress responses, metabolism, photosynthesis, transport and signal transduction, showed increased abundance upon SA treatment. Susceptible plants showed characteristic yellow mosaic symptoms upon MYMIV infection. A concentration dependent decrease in physiological symptoms associated with MYMIV was observed upon exogenous SA treatment prior to viral inoculation; and no visible symptom was observed at 100 μM SA. SA treatment stimulated SOD and GPX activity and inhibited CAT activity thus preventing ROS mediated damage. Significant increase in chlorophyll, protein, carbohydrate, phenolic content and H(2)O(2) were observed. Involvement of calmodulin for transmission of defense signal by SA is suggested. A metabolic reprogramming leading to enhanced synthesis of proteins involved in primary and secondary metabolisms is necessary for SA mediated resistance to MYMIV. Identification of proteins showing increased abundance, involved in photosynthetic process is a significant finding which restores virus-induced degradation of the photosynthetic apparatus and provides enhanced metabolites required for repartition of resources towards defense.

  3. Combining ability analysis for yield and its attributes in Blackgram (Vigna mungo (L. Hepper

    Directory of Open Access Journals (Sweden)

    A.V.S. Durga Prasad and E. Murugan

    2015-06-01

    Full Text Available Hybridization in Line x Tester mating design was conducted with 11 MYMV susceptible lines and three resistant testers in blackgram (Vigna mungo (L. Hepper to understand the nature of gene action, combining ability of the parents (gca and to assess the potential for the exploitation of heterosis (sca in hybrids. Data on nine quantitative characters viz.,days to 50 per cent flowering, plant height (cm, number of branches per plant, number of clusters per plant, number of pods per plant, number of seeds per pod, hundred seed weight (g and seed yield per plant (g were collected on 33 hybrids and their 11 parents. Among the genotypes, PU 31, LBG 645, ADT 3, CO 6 and LBG 709 recorded relatively high per se performance and gca effects for majority of seed yield attributing traits. The hybrid, LBG 709 x PU 31 followed by CO 6 x PU 31 and LBG 645 x VBN (Bg 6 exhibited significant high per se and sca for most of the traits viz., number of branches per plant, number of clusters per plant, number of pods per plant, pod length and seed yield per plant .The crosses involving the parents, LBG 645 x PU 31 and ADT 3 x PU 31 recorded significant gca and non-significant sca effect for most of the characters inferring that these crosses would produce superior recombinants for seed yield.

  4. Purification and characterization of catalase from sprouted black gram (Vigna mungo) seeds.

    Science.gov (United States)

    Kandukuri, Sai Srikar; Noor, Ayesha; Ranjini, S Shiva; Vijayalakshmi, M A

    2012-03-15

    Black gram (Vigna mungo) is a legume which belongs to Fabaceae family. It is a rich source of protein. It has been known to have interesting small molecule antioxidant activity. However, its enzymatic antioxidant properties have not been explored much. In the present work we studied catalase, a principal antioxidant enzyme from black gram seeds. Day four sprouted black gram seeds were found to have a significant catalase content approximately of 15,240 U/g seeds. IMAC (Seph 4B-IDA-Zn(II)) was used for purifying this catalase, a purification fold of 106 and a high specific activity of 25,704 U/mg was obtained. The K(m) and V(max) of the purified catalase were found to be 16.2 mM and 2.5 μmol/min. The effect of inhibitors like Sodium azide (NaN(3)) and EDTA and different metal ions on catalase activity were studied. NaN(3), Fe(3+)and Cu(2+) were found to have profound inhibitory effects on the enzyme activity. Other metal ions like Ni(2+), Ca(2+), Mg(2+) and Mn(2+) had both enhancing and inhibitory effects. The enzyme showed optimal activity at a temperature of 40°C and pH 7.0. It was stable over a broad range of pH 6.0-10.0 and had a half life of 7h 30 min at 50°C.

  5. Stable genetic transformation of Vigna mungo L. Hepper via Agrobacterium tumefaciens.

    Science.gov (United States)

    Saini, R; Sonia; Jaiwal, P K; Jaiwal, S

    2003-06-01

    Vigna mungo is one of the large-seeded grain legumes that has not yet been transformed. We report here for the first time the production of morphologically normal and fertile transgenic plants from cotyledonary-node explants inoculated with Agrobacterium tumefaciens carrying binary vector pCAMBIA2301, the latter of which contains a neomycin phosphotransferase ( nptII) gene and a beta-glucuronidase (GUS) gene ( uidA) interrupted with an intron. The transformed green shoots, selected and rooted on medium containing kanamycin, tested positive for nptII and uidA genes by polymerase chain reaction (PCR) analysis. These shoots were established in soil and grown to maturity to collect the seeds. Mechanical wounding of the explants prior to inoculation with Agrobacterium, time lag in regeneration due to removal of the cotyledons from explants and a second round of selection at the rooting stage were found to be critical for transformation. Analysis of T(0) plants showed the expression and integration of uidA into the plant genome. GUS activity in leaves, roots, flowers, anthers and pollen grains was detected by histochemical assay. PCR analysis of T(1) progeny revealed a Mendelian transgene inheritance pattern. The transformation frequency was 1%, and 6-8 weeks were required for the generation of transgenics.

  6. Screening three cultivars of Vigna mungo L. against ozone by application of ethylenediurea (EDU).

    Science.gov (United States)

    Singh, Shalini; Agrawal, S B; Singh, Poonam; Agrawal, M

    2010-10-01

    Three Indian black gram cultivars (Vigna mungo L. cv. Barkha, Shekhar and TU-94-2) were grown at a tropical suburban site in Varanasi, India to evaluate the varietal differences in response to ambient O(3) under field conditions using ethylenediurea (EDU). EDU (400 ppm) was given as soil drench at 10-day intervals during the growth period of the cultivars. O(3) monitoring data clearly showed high concentrations with a mean value ranging between 41.3 and 59.9 ppb. EDU treatment caused significant increases in various growth parameters and total biomass accumulation in Barkha and Shekhar. EDU caused retention of more biomass in leaves during vegetative period and translocated more photosynthates towards reproductive parts, which resulted into yield enhancement. Weight of seeds plant(-1) was higher by 36.4% and 35.6% in Barkha and Shekhar, respectively, treated with EDU compared to non-EDU-treated plants. However, TU-94-2 did not exhibit any significant difference in weight of seeds plant(-1). Starch, total sugar, amino acids and K contents increased in seeds of EDU-treated plants leading to improvement in quality response index (QRI) of seeds. EDU helped in identifying the cultivar susceptibility to O(3) stress and therefore is very useful as a monitoring tool to assess the impact of ambient O(3) on plants under natural field conditions particularly in areas experiencing moderate concentrations of O(3).

  7. Molecular characterization of urdbean (Vigna mungo) germplasm related to resistance against urdbean leaf crinkle virus.

    Science.gov (United States)

    Binyamin, R; Aslam Khan, M; Khan, A I; Azam Khan, M; Awan, F S; Khan, N A

    2011-01-01

    Urdbean (Vigna mungo) is an important pulse crop grown worldwide. Urdbean leaf crinkle virus (ULCV) is a pathogen of urdbean found in Pakistan that causes huge losses in yield. Forty urdbean varieties/lines were screened against the virus under field conditions during spring season 2009. None of the lines appeared to be highly resistant or resistant. On the basis of a 0-5 disease rating scale and disease severity index, genotypes varied significantly in their reaction to ULCV. Four lines (M-6206, IAM-382-15, IAM-133, and Mash-1) were moderately resistant, eight were rated as moderately susceptible, and 21 as susceptible; the remaining seven lines were highly susceptible. RAPD analyses revealed an extensive amount of variation, which could be used for cultivar identification. Genetic differentiation among urdbean genotypes was similar to the field screening data. The varieties 6065-3 and 6206 were highly susceptible and moderately resistant, respectively, to ULCV under field conditions, confirmed by the RAPD analysis. These varieties were the most diverse varieties in the similarity matrix (67.2%), while the varieties IAM-382-9 and 07M003 were the most similar (98.4%). This information will help in the recognition of available resistant germplasms that can resist this disease and will be utilized for urdbean improvement in Pakistan.

  8. Regeneration of Blackgram (Vigna mungo L. on Changes of Hormonal Condition

    Directory of Open Access Journals (Sweden)

    Shariful Alam MONY

    2010-12-01

    Full Text Available The study comprised of experiments for shoot regeneration and plantlet formation from cotyledonary node of Vigna mungo by culturing them on low concentration of BAP followed by transfer to hormone free MS medium. Cotyledonary node explants were cultured on different concentrations of BAP (0, 1, 2.5 and 50 mg l-1. Shoot regeneration occurred from cotyledonary nodes irrespective of the presence or absence of BAP in the medium. However, culture of cotyledonary node explants for 10 days on medium containing 1.0 mg l-1 BAP followed by transfer to hormone free medium gave higher number of shoots (9.33/explant compared to culture of the explants on hormone free medium for 15 days followed by transfer to medium containing 1.0 mg l-1 BAP (8.33/explants. The regenerated shoots were transferred to rooting medium supplemented with different concentrations of IBA and NAA. The high frequency (100.0% of rooting was observed with MS medium supplemented with 0.5 mg l-1 IBA. The rooted plants were transferred to pots for hardening.

  9. ANTI-INFLAMMATORY, ANALGESIC AND ULCEROGENIC ACTIVITY OF VIGNA MUNGO LINN. LEAVES

    Directory of Open Access Journals (Sweden)

    Md.Rageeb Md.Usman

    2012-01-01

    Full Text Available The anti-inflammatory, analgesic and ulcerogenic activity of extract of leaves of Vigna Mungo linn. (Leguminosae were investigated as well as the mechanisms of action. The extract significantly (P<0.05 inhibited the formation of paw edema induced by    carrageenan in rat and increased reaction latency to thermal pain in rat in a dose-dependent manner. The extract caused a significant (P<0.05 dose-dependent ulceration of rat gastric mucosa and concentration-dependent inhibition of hypotonicity-induced haemolysis of red blood cell. Also the extract significantly (P<0.05 inhibited the activates of the phospholipase A2 and prostaglandine synthesis in a concentration related manner. These suggest that the leaves posses anti-inflammatory, analgesic and ulcerogenic activities mediated through sequential inhibition of the enzymes responsible for prostaglandine synthesis from arachidonic acid. Phytochemical analysis of the extract revealed the presence of glycoside, tannins, alkaloid, flavonoids and Saponins. Acute toxicity studies established an oral LD50 greater than 3000 mg/kg.

  10. Ameliorating Effects of Iron and Zinc on Vigna mungo L. Treated with Tannery Effluent

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    Shefali Srivastava

    2014-01-01

    Full Text Available Different dilutions, that is, 25, 50, 75, and 100%, of tannery effluent (TE were chosen for the present study to assess the phytotoxic effects on Vigna mungo L. For amelioration purposes, different levels and combinations of iron and zinc were supplied to the plants along with 50% TE that is chosen on the basis of prior test under Petri dish culture. Cytotoxic and biochemical analysis and plant tolerance index (PTI of plant were observed. Mitotic index deceased with increase in effluent concentration whereas abnormality % was increased. The pigments (chlorophyll a, total, and carotenoids were decreased with increasing treatment levels of TE at both growth stages. However, carotenoid content increased significantly at all dilution levels of TE after first growth stage. Chlorophyll b was increased significantly after 35 days of growth but decreased after 70 days. The protein contents were also significantly decreased with increase in all TE treatments and increased significantly in zinc recovery treatments. Activities of catalase and peroxidase enzymes were significantly affected and increased significantly with effluent treatments. PTI showed an enhanced tolerance capacity of plant with treatment of iron and zinc. A negative correlation was found (r=-0.97 between plant height and different dilutions of effluent whereas it was positively correlated (r=0.95 with iron and zinc treatments. The study represents the ameliorative effect of iron and zinc for phytotoxic damage in V. mungo caused by tannery effluent.

  11. Zinc and copper induced changes in physiological characteristics of Vigna mungo (L.).

    Science.gov (United States)

    Solanki, Radha; Anju; Poonam; Dhankhar, R

    2011-11-01

    The effect of deleterious concentration of zinc and copper provided either individually or in combination in the nutrient media was investigated in order to assess the effect of metal interaction in Vigna mungo (L.). Both metals showed negative effect and led to a marked decrease in seed germination (20%), seedling growth (91.7%) and nitrate reductase activity (85.7%) with the increase in metal concentrations. The present study also emphasizes on the response of catalase and peroxidase enzyme under zinc and copper stress. Both antioxidant enzymes exhibited an increasing trend under different treatment conditions but it was reverse at highly toxic metal concentration. The results showed active involvement of peroxidase enzyme in regulating oxidative stress rather than catalase enzyme, as the specific activity of peroxidase enzyme got increased by 8.94% under the combined metals stress whereas catalase activity got declined by 60.97% in comparison to control due to excessive stress. The combined effect of copper and zinc metal was more pronounced in comparison to their individual effects.

  12. Immunostimolatory activities of Vigna mungo L. extract in male Sprague-Dawley rats.

    Science.gov (United States)

    Solanki, Yogendrasinh B; Jain, Sunita M

    2010-01-01

    Vigna mungo L. (Fabaceae) is a popular food legume used in the traditional Indian system of medicine for the treatment of a variety of disease conditions. The objective of the study was to evaluate any immunostimulatory activities of the extract of V. mungo seeds in an animal model. The induction of any immunostimulatory effects were evaluated using measures of sheep red blood cells (SRBC)-induced humoral antibody titer, SRBC-induced delayed-type hypersensitivity (DTH), neutrophil adhesion, and in vivo phagocytosis (via the carbon clearance method) after host treatment with the extract. The results here indicated that primary and secondary antibody titers in the rats were significantly increased by treatment with the V. mungo extract as compared with those noted among rats in a control group. Increases in DTH response, the percentage (%) neutrophil adhesion, and in situ phagocytosis were also observed after treatment with the extract. We summarize that the apparent immunostimulatory effect of the V. mungo seed extract might be attributed to an augmentation of humoral and cell-mediated responses, phagocytosis, and hematopoiesis in the treated rats. The findings in this study suggest that V. mungo seed extract possesses profound immunostimulatory activities. Whether such outcomes are also evidenced by consumption of the intact seeds themselves, as is most likely to be the case with humans, remains to be determined. Nonetheless, the present study provides evidence that could help explain how the traditional use of V. mungo has been successful in the treatment of various disorders in humans.

  13. Antioxidant Status of Vigna mungo L. in Response to Sulfur Nutrition

    Directory of Open Access Journals (Sweden)

    Neelam Chandra

    2014-01-01

    Full Text Available Black gram (Vigna mungo L. var. DPU-88-31, an edible legume, was grown at 1, 2, 4, 6, and 8 meq S L−1 to study the effect of deficient and excess level of sulfur on oxidative metabolism. Plants supplied by 4 meq S L−1 showed optimum yield. Sulfur deficient plants (1 and 2 meq S L−1 showed reduction in growth and chlorosis of young leaves. Tissue sulfur and cysteine concentration was increased with increasing sulfur supply. The thresholds for critical concentration of sulfur deficiency and toxicity were 0.315% and 0.434% dry weight. Biomass and photoassimilatory pigments were decreased and carbohydrates (sugar and starch were accumulated in leaves of sulfur deficient and excess plants. Accumulation of hydrogen peroxide and thiobarbituric acid reactive substances in sulfur deficient and excess plants caused oxidative damage in plants which was also evident by the increase in the activity of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, glutathione reductase, and concentration of ascorbate and nonprotein thiols.

  14. Vigna subterranea ammonium transporter gene (VsAMT1: Some bioinformatics insights

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    Adewole T. Adetunji

    2015-12-01

    Full Text Available Ammonium transporters (AMTs play a role in the uptake of ammonium, the form in which nitrogen is preferentially absorbed by plants. Vigna subterranea (VsAMT1 and Solanum tuberosum (StAMT1 AMT1s were characterized using molecular biology and bioinformatics methods. AMT1-specific primers were designed and used to amplify the AMT1 internal regions. Nucleotide sequencing, alignment and phylogenetic analysis assigned VsAMT1 and StAMT1 to the AMT1 family. The deduced amino acid sequences showed that VsAMT1 is 92% and 89% similar to Phaseolus vulgaris PvAMT1.1 and Glycine max AMT1 respectively, while StAMT1 is 92% similar to Solanum lycopersicum LeAMT1.1, and correspond to the 5th–10th trans-membrane domains. Residues VsAMT1 D23 and StAMT1 D15 are predicted to be essential for ammonium transport, while mutations of VsAMT1 W1A-L and S87A and StAMT1 S76A may further enhance ammonium transport. In addition to nitrogen uptake from the roots, VsAMT1 may also contribute to interactions with rhizobia.

  15. APPRAISAL OF GENE ACTION FOR INDETERMINATE GROWTH IN MUNGBEAN [VIGNA RADIATA (L. WILCZEK

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    Javed eIqbal

    2015-09-01

    Full Text Available In order to investigate the inheritance pattern of indeterminate growth in Vigna radiata, various related traits were studied. The techniques implied for the purpose were generation mean and variance analyses. Narrow sense heritability estimates were also computed. Four out of fifty greengram accessions were selected during preliminary screen trial based on DDd2 and DDh2 values. Two cross combinations were made with the help of four parents. Generation variance analysis demonstrated the engagement of additive and environmental components, with the pre-pondrance of additive gene action. Narrow sense heritability estimates (>67% also supported the same. In generation mean analysis both the cross combinations manifested non-allelic epistatic digenic interactions for the investigated traits except for plant height at first flower initiation in both the crosses and for seed yield per plant in one cross, where only additive and dominance components were important. For pyramiding the additive genes which favour determinate plant growth and higher harvest index and simultaneously purging the genes promoting twining growth habit escorted with low seed yield, any modified breeding scheme which could serve the said purpose may be opted.

  16. Nutritional Assessment and Antioxidant Activities of Different Varieties of Vigna radiata

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    Riaz Ullah

    2014-01-01

    Full Text Available Three cultivars of Vigna radiata, namely, NM-92, NM-98, and NM-06, were analyzed for their proximate composition. The samples were also tested by HPLC for amino acid content. The data showed that all the varieties had same moisture level. The maximum ash content (4.29% was present in NM-92, and crude fat (2.26% was highest in NM-98 while NM-06 contained maximum amount of crude protein. About eighteen types of amino acids were detected in each of the three varieties. Acidic amino acids, that is, aspartic and glutamic acids, were in considerable amount ranged from 13 to 23% followed by leucine, isoleucine, alanine, valine, lysine, phenyl alanine, serine, and arginine which fell in the range of 3–8% of total protein. The maximum amount (13.00 and 22.80% of aspartic and glutamic acids was present in NM-98. Similarly arginine (6.83% and serine (5.45% were also in highest amount in this variety. Leucine (7.46% was maximum in NM-92 variety. NM-06 contained almost all the amino acids in lesser quantity except for few like threonine, proline, glycine, and alanine. It was concluded from the present study that varieties were of different nutritional value and HPLC was a sensitive method for amino acids determination. Antioxidant activities of all three varieties were also assayed and showed significant results.

  17. Reduction in flatulence factors in mung beans (Vigna radiata) using low-dose gamma-irradiation

    International Nuclear Information System (INIS)

    Mungbeans (Vigna radiata), control and gamma-irradiated at insect disinfestation dose levels (0.25 and 0.75 kGy) were germinated (0-6 Bays) and the qualitative and quantitative changes in soluble carbohydrates were studied in detail. The key flatulence-producing raffinose family oligosaccharides inmungbeans were degraded in the irradiated samples at the onset of the germination (0-2 days) compared to the control where it occurred much later (>4days). However, the reducing sugars, mainly glucose, fructose and galactose, which are metabolised easily, were enhanced in the irradiated samples. At low dose (0.25 kGy), irradiation had no effect on germination and sprout length, indicating that irradiated beans are suitable for use as sprouted beans. These observations clearly indicate that gamma-irradiation at insect disinfestation dose levels improved the digestibility and nutritional quality of mung beans by reducing the content of oligosaccharides responsible for intestinal gas production. (C) 1999 Society of Chemical Industry

  18. De novo transcriptome assembly of two Vigna angularis varieties collected from Korea

    Directory of Open Access Journals (Sweden)

    Yeonhwa Jo

    2016-06-01

    Full Text Available The adzuki bean (Vigna angularis, a member of the family Fabaceae, is widely grown in Asia, from East Asia to the Himalayas. The adzuki bean is known as an ingredient that adds sweetness to diverse desserts made in Eastern Asian countries. Libraries prepared from two V. angularis varieties referred to as Taejin Black and Taejin Red were paired-end sequenced using the Illumina HiSeq 2000 system. The raw data in this study can be available in NCBI SRA database with accession numbers of SRR3406660 and SRR3406553. After de novo transcriptome assembly using Trinity, we obtained 324,219 and 280,056 transcripts from Taejin Black and Taejin Red, respectively. We predicted a total of 238,321 proteins and 179,519 proteins for Taejin Black and Taejin Red, respectively, by the TransDecoder program. We carried out BLASTP on the predicted proteins against the Swiss-Prot protein sequence database to predict the putative functions of identified proteins. Taken together, we provide transcriptomes of two adzuki bean varieties by RNA-Seq, which might be usefully applied to generate molecular markers.

  19. De novo transcriptome assembly of two Vigna angularis varieties collected from Korea.

    Science.gov (United States)

    Jo, Yeonhwa; Lian, Sen; Cho, Jin Kyong; Choi, Hoseong; Kim, Sang-Min; Kim, Sun-Lim; Lee, Bong Choon; Cho, Won Kyong

    2016-06-01

    The adzuki bean (Vigna angularis), a member of the family Fabaceae, is widely grown in Asia, from East Asia to the Himalayas. The adzuki bean is known as an ingredient that adds sweetness to diverse desserts made in Eastern Asian countries. Libraries prepared from two V. angularis varieties referred to as Taejin Black and Taejin Red were paired-end sequenced using the Illumina HiSeq 2000 system. The raw data in this study can be available in NCBI SRA database with accession numbers of SRR3406660 and SRR3406553. After de novo transcriptome assembly using Trinity, we obtained 324,219 and 280,056 transcripts from Taejin Black and Taejin Red, respectively. We predicted a total of 238,321 proteins and 179,519 proteins for Taejin Black and Taejin Red, respectively, by the TransDecoder program. We carried out BLASTP on the predicted proteins against the Swiss-Prot protein sequence database to predict the putative functions of identified proteins. Taken together, we provide transcriptomes of two adzuki bean varieties by RNA-Seq, which might be usefully applied to generate molecular markers.

  20. Involvement of gibberellins in expression of a cysteine proteinase (SH-EP) in cotyledons of Vigna mungo seedlings.

    Science.gov (United States)

    Taneyama, M; Okamoto, T; Yamane, H; Minamikawa, T

    2001-11-01

    The expression of a papain-type proteinase, designated SH-EP, in cotyledons of Vigna mungo seedlings has been shown to require some factors in the embryonic axes. Gibberellin A1 (GA(1)) and GA(20) were identified by GC-MS in embryonic axes of V. mungo seedlings. The level of accumulation of SH-EP in cotyledons of V. mungo seedlings was greatly reduced by treatment of the seeds with uniconazole-P, an inhibitor for GA biosynthesis. The reduced level of accumulation of SH-EP in cotyledons by uniconazole-P was recovered by exogenous application of GA(1) and GA(20) to the seedlings.

  1. Occurrence of a Lysogenic Streptomyces sp. on the Nodule Surface of Black Gram (Vigna mungo (L.) Hepper).

    Science.gov (United States)

    Rangarajan, M; Ravindran, A D; Hariharan, K

    1984-07-01

    A lysogenic Streptomyces sp., strain NS.A4, which was isolated from the nodule surface of black gram (Vigna mungo (L.) Hepper), was found to inhibit rhizobia of fast-and slow-growing strains of cowpeas and soybeans. It exhibited plaques when there was a change in cultural conditions. Repeated culturing of the organism in nutrient agar and broth confirmed the infection of Streptomyces sp. strain NS.A4 by an actinophage. Addition of the culture filtrate of Streptomyces sp. strain NS.A4 to shaken broth cultures of three other Streptomyces spp. resulted in phage infection.

  2. Distribution of phytopathogenic bacteria in infested seeds

    Science.gov (United States)

    Populations of phytopathogenic bacteria representing five host-pathogen combinations were assessed to determine if there was a mathematical relationship common across seedborne bacterial diseases. Bacterial populations were estimated from naturally-infested seeds of cowpea (Vigna unguiculata), peppe...

  3. Seed quality in informal seed systems

    NARCIS (Netherlands)

    Biemond, P.C.

    2013-01-01

    Keywords:     informal seed systems, seed recycling, seed quality, germination, seed pathology, seed health, seed-borne diseases, mycotoxigenic fungi, Fusarium verticillioides, mycotoxins, Vigna unguiculata, Zea mays, Nigeria.   Seed is a crucial input for agricultural producti

  4. Foraging behaviour of the egg parasitoid Uscana lariophaga towards biological control of bruchid pests in stored cowpea in West Africa.

    NARCIS (Netherlands)

    Alebeek, van F.A.N.

    1996-01-01

    Seed beetles cause considerable losses in traditionally stored cowpea ( Vigna unguiculata Walp.) under subsistence farming conditions in West Africa. The indigenous egg parasitoid Uscana lariophaga Steffan (Hymenoptera; Trichogrammatidae) is evaluated as a candidate for a conservation strategy of bi

  5. Dispersal and functional response of Uscana lariophaga in two different habitats: stored cowpea pods and seeds

    NARCIS (Netherlands)

    Alebeek, van F.A.N.; Antwi, K.K.; Huis, van A.; Lenteren, van J.C.

    2007-01-01

    The effect of two different habitats, stored cowpea [Vigna unguiculata (L.) Walpers] pods and seeds, on the dispersal and functional response of Uscana lariophaga Steffan (Hymenoptera Trichogrammatidae) was investigated, with Callosobruchus maculatus (F.) (Coleoptera Bruchidae) eggs as hosts. In the

  6. Mobile phone radiation inhibits Vigna radiata (mung bean) root growth by inducing oxidative stress

    International Nuclear Information System (INIS)

    During the last couple of decades, there has been a tremendous increase in the use of cell phones. It has significantly added to the rapidly increasing EMF smog, an unprecedented type of pollution consisting of radiation in the environment, thereby prompting the scientists to study the effects on humans. However, not many studies have been conducted to explore the effects of cell phone EMFr on growth and biochemical changes in plants. We investigated whether EMFr from cell phones inhibit growth of Vigna radiata (mung bean) through induction of conventional stress responses. Effects of cell phone EMFr (power density: 8.55 μW cm-2; 900 MHz band width; for 1/2, 1, 2, and 4 h) were determined by measuring the generation of reactive oxygen species (ROS) in terms of malondialdehyde and hydrogen peroxide (H2O2) content, root oxidizability and changes in levels of antioxidant enzymes. Our results showed that cell phone EMFr significantly inhibited the germination (at ≥2 h), and radicle and plumule growths (≥1 h) in mung bean in a time-dependent manner. Further, cell phone EMFr enhanced MDA content (indicating lipid peroxidation), and increased H2O2 accumulation and root oxidizability in mung bean roots, thereby inducing oxidative stress and cellular damage. In response to EMFr, there was a significant upregulation in the activities of scavenging enzymes, such as superoxide dismutases, ascorbate peroxidases, guaiacol peroxidases, catalases and glutathione reductases, in mung bean roots. The study concluded that cell phone EMFr inhibit root growth of mung bean by inducing ROS-generated oxidative stress despite increased activities of antioxidant enzymes.

  7. Mobile phone radiation inhibits Vigna radiata (mung bean) root growth by inducing oxidative stress

    Energy Technology Data Exchange (ETDEWEB)

    Sharma, Ved Parkash [Department of Environment and Vocational Studies, Panjab University, Chandigarh 160014 (India); Department of Zoology, Panjab University, Chandigarh 160014 (India); Singh, Harminder Pal, E-mail: hpsingh_01@yahoo.com [Department of Environment and Vocational Studies, Panjab University, Chandigarh 160014 (India); Kohli, Ravinder Kumar; Batish, Daizy Rani [Department of Botany, Panjab University, Chandigarh 160014 (India)

    2009-10-15

    During the last couple of decades, there has been a tremendous increase in the use of cell phones. It has significantly added to the rapidly increasing EMF smog, an unprecedented type of pollution consisting of radiation in the environment, thereby prompting the scientists to study the effects on humans. However, not many studies have been conducted to explore the effects of cell phone EMFr on growth and biochemical changes in plants. We investigated whether EMFr from cell phones inhibit growth of Vigna radiata (mung bean) through induction of conventional stress responses. Effects of cell phone EMFr (power density: 8.55 {mu}W cm{sup -2}; 900 MHz band width; for 1/2, 1, 2, and 4 h) were determined by measuring the generation of reactive oxygen species (ROS) in terms of malondialdehyde and hydrogen peroxide (H{sub 2}O{sub 2}) content, root oxidizability and changes in levels of antioxidant enzymes. Our results showed that cell phone EMFr significantly inhibited the germination (at {>=}2 h), and radicle and plumule growths ({>=}1 h) in mung bean in a time-dependent manner. Further, cell phone EMFr enhanced MDA content (indicating lipid peroxidation), and increased H{sub 2}O{sub 2} accumulation and root oxidizability in mung bean roots, thereby inducing oxidative stress and cellular damage. In response to EMFr, there was a significant upregulation in the activities of scavenging enzymes, such as superoxide dismutases, ascorbate peroxidases, guaiacol peroxidases, catalases and glutathione reductases, in mung bean roots. The study concluded that cell phone EMFr inhibit root growth of mung bean by inducing ROS-generated oxidative stress despite increased activities of antioxidant enzymes.

  8. Sphingobacterium pakistanensis sp. nov., a novel plant growth promoting rhizobacteria isolated from rhizosphere of Vigna mungo.

    Science.gov (United States)

    Ahmed, Iftikhar; Ehsan, Muhammad; Sin, Yeseul; Paek, Jayoung; Khalid, Nauman; Hayat, Rifat; Chang, Young H

    2014-02-01

    The taxonomic status of a bacterium, strain NCCP-246(T), isolated from rhizosphere of Vigna mungo, was determined using a polyphasic taxonomic approach. The strain NCCP-246(T) can grow at 16-37 °C (optimum 32 °C), at pH ranges of 6-8 (optimum growth occurs at pH 7) and in 0-4 % (w/v) NaCl. Phylogenetic analysis based upon on 16S rRNA gene sequence comparison revealed that strain NCCP-246(T) belonged to genus Sphingobacterium. Strain NCCP-246(T) showed highest similarity to the type strain of Sphingobacterium canadense CR11(T) (97.67 %) and less than 97 % with other species of the genus. The DNA-DNA relatedness value of strain NCCP-246(T) with S. canadense CR11(T) and Sphingobacterium thalpophilum JCM 21153(T) was 55 and 44.4 %, respectively. The chemotaxonomic data revealed the major menaquinone as MK-7 and dominant cellular fatty acids were summed feature 3 [C16:1 ω7c/C16:1 ω6c] (37.07 %), iso-C15:0 (28.03 %), C16:0 (11.85 %), C17:0 cyclo (8.84 %) and C14:0 (2.42 %). The G+C content of the strain was 39.2 mol%. On the basis of DNA-DNA hybridization, phylogenetic analyses, physiological and, biochemical data, strain NCCP-246(T) can be differentiated from the validly named members of genus Sphingobacterium and thus represents as a new species, for which the name, Sphingobacterium pakistanensis sp. nov. is proposed with the type strain NCCP-246(T) (= JCM18974 (T) = KCTC 23914(T)).

  9. Synthesis and Posttranslational Activation of Sulfhydryl-Endopeptidase in Cotyledons of Germinating Vigna mungo Seeds.

    Science.gov (United States)

    Mitsuhashi, W; Minamikawa, T

    1989-01-01

    A sulfhydryl-endopeptidase was purified as a 33 kilodalton (kD) mass polypeptide from cotyledons of Vigna mungo seedlings. Immunoblot analysis with antiserum made against the purified enzyme showed that the sulfhydryl-endopeptidase was synthesized only in the cotyledons during germination and that the amount of the enzyme increased until 4 days after imbibition and decreased thereafter. Next, an RNA fraction was prepared from cotyledons of 3 day old seedlings and translated in a wheat germ system. The synthesis of a 45 kD polypeptide was shown by the analysis of its translation products by immunoprecipitation with the antiserum to the endopeptidase and gel electrophoresis. When the RNA fraction was translated in the presence of canine microsomal membranes, a smaller polypeptide, having a 43 kD molecular mass, was detected as the translation product. When membrane-bound polysomes, but not free polysomes, prepared from cotyledons were used for translation in the wheat germ system, both the 43 and 45 kD polypeptides were synthesized. By incubation of a crude enzyme extract from cotyledons at 5 +/- 1 degrees C at neutral pH, the 43 kD polypeptide was sequentially cleaved to the 33 kD polypeptide via 39 and 36 kD intermediate polypeptides. The endopeptidase was activated simultaneously with the processing. Two-dimensional polyacrylamide gel electrophoresis showed that the 33 kD polypeptide was the fully activated form of the enzyme, whereas little or no activity was detected in other forms. From the present results, we postulate that the sulfhydryl-endopeptidase is first synthesized as the 45 kD precursor with a 2 kD signal peptide being cleaved, and that the 43 kD polypeptide is further cleaved to give the 33kD mature enzyme.

  10. Synthesis and Posttranslational Activation of Sulfhydryl-Endopeptidase in Cotyledons of Germinating Vigna mungo Seeds 1

    Science.gov (United States)

    Mitsuhashi, Wataru; Minamikawa, Takao

    1989-01-01

    A sulfhydryl-endopeptidase was purified as a 33 kilodalton (kD) mass polypeptide from cotyledons of Vigna mungo seedlings. Immunoblot analysis with antiserum made against the purified enzyme showed that the sulfhydryl-endopeptidase was synthesized only in the cotyledons during germination and that the amount of the enzyme increased until 4 days after imbibition and decreased thereafter. Next, an RNA fraction was prepared from cotyledons of 3 day old seedlings and translated in a wheat germ system. The synthesis of a 45 kD polypeptide was shown by the analysis of its translation products by immunoprecipitation with the antiserum to the endopeptidase and gel electrophoresis. When the RNA fraction was translated in the presence of canine microsomal membranes, a smaller polypeptide, having a 43 kD molecular mass, was detected as the translation product. When membrane-bound polysomes, but not free polysomes, prepared from cotyledons were used for translation in the wheat germ system, both the 43 and 45 kD polypeptides were synthesized. By incubation of a crude enzyme extract from cotyledons at 5 ± 1°C at neutral pH, the 43 kD polypeptide was sequentially cleaved to the 33 kD polypeptide via 39 and 36 kD intermediate polypeptides. The endopeptidase was activated simultaneously with the processing. Two-dimensional polyacrylamide gel electrophoresis showed that the 33 kD polypeptide was the fully activated form of the enzyme, whereas little or no activity was detected in other forms. From the present results, we postulate that the sulfhydryl-endopeptidase is first synthesized as the 45 kD precursor with a 2 kD signal peptide being cleaved, and that the 43 kD polypeptide is further cleaved to give the 33kD mature enzyme. Images Figure 1 Figure 2 Figure 4 Figure 5 Figure 6 PMID:16666526

  11. Combining ability analysis for yield and its component traits in Blackgram (Vigna mungo (L. Hepper

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    Y.Anbu Selvam and R.Elangaimannan

    2010-12-01

    Full Text Available A diallele analaysis of 8 x 8 was studied in black gram (Vigna mungo with a view to understand the combining ability, nature of gene action andpotential for the exploitation of heterosis using nine quantitative characters viz., days to first flower, plant height, number of branches per plant,number of clusters per plant, number of pods per cluster, number of pods per plant, number of seeds per pod, hundred seed weight and seed yieldper plant in 28 hybrids and their eight parents. The parent ADT 3 recorded relatively high per se performance for seed yield, hundred seedweight, number of clusters per plant, number of pods per cluster, number of pods per plant and number of branches per plant with early floweringand short stature. Among the hybrids, ADT 3/99V48, ADT 3/LBG 645 and 2KU 53/LBG 645 were found to have high mean performance foralmost all the yield component characters besides seed yield. The gca effect of the genotypes ADT 3, LBG 645 and 2 KU 53 was high andsignificant for the traits seed yield per plant besides number of pods per cluster, number of pods per plant and number of seeds per pod. The SCAvariance was greater than GCA variance for all the traits studied thus indicating the predominance of non-additive gene action. Among thehybrids, ADT 3/99V48 recorded the maximum significant sca effect indicating their suitability for heterosis breeding. The hybrids ADT 3/ LBG645 and 2 KU 53/ LBG 645 recorded non significant sca effects for almost all the traits studied including seed yield per plant. Hence, these twocrosses were adjudged as superior for recombination breeding based on sca effects

  12. Phosphorus Response and Amino Acid Composition of Different Green Gram (Vigna radiata L. Genotypes from Myanmar

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    M. Kywe

    2007-10-01

    Full Text Available Mungbean or green gram (Vigna radiata L. is an important component of rice-based cropping systems in Myanmar, where grain yields of around 800 kg ha^(-1 are much below its yield potential of 3000 kg ha^(-1. The reasons for this shortfall are as under-investigated as is the genotype-specific response of this crop to phosphorus (P application, which is critically low in many Myanmar soils, and the genetic variation in grain quality. For green gram quality, the concentration of lysine, an essential amino acid is particularly important given its scarcity in many cereal-based diets of Southeast Asia. The purpose of this study therefore was to investigate the effects of P application on the root and shoot growth, yield and its components for a range of green gram varieties, and to analyse the protein concentration and amino acid composition in green gram seed of different origins. To this end from 2001 to 2003, field experiments were conducted under rain-fed conditions in Yezin and Nyaung Oo. Fifteen landraces and five introduced green gram cultivars were grown at two levels of P (0 and 15 kg ha^(-1. There were large genotypic differences in P effects and a significant interaction between green gram genotypes and P for shoot and root growth. An unexpected benefit of P application was a reduction of pest and plant virus infestation in the field. Significant genotypic differences in the amino acid profile of seeds were also observed. The results indicate the potential for breeding efforts to increase seed yield and protein quality in green gram.

  13. Influence of distillery effluent on germination and growth of mung bean (Vigna radiata) seeds

    International Nuclear Information System (INIS)

    Distillery effluent or spent wash discharged as waste water contains various toxic chemicals that can contaminate water and soil and may affect the common crops if used for agricultural irrigation. Toxic nature of distillery effluent is due to the presence of high amounts of organic and inorganic chemical loads and its high-acidic pH. Experimental effects of untreated (Raw) distillery effluent, discharged from a distillery unit (based on fermentation of alcohol from sugarcane molasses), and the post-treatment effluent from the outlet of conventional anaerobic treatment plant (Treated effluent) of the distillery unit were studied in mung bean (Vigna radiata, L.R. Wilczek). Mung bean is a commonly used legume crop in India and its neighboring countries. Mung bean seeds were presoaked for 6 h and 30 h, respectively, in different concentrations (5-20%, v/v) of each effluent and germination, growth characters, and seedling membrane enzymes and constituents were investigated. Results revealed that the leaching of carbohydrates and proteins (solute efflux) were much higher in case of untreated effluent and were also dependent to the presoaking time. Other germination characters including percentage of germination, speed of germination index, vigor index and length of root and embryonic axis revealed significant concentration-dependent decline in untreated effluent. Evaluation of seedlings membrane transport enzymes and structural constituents (hexose, sialic acid and phospholipids) following 6 h presoaking of seeds revealed concentration-dependent decline, which were much less in treated effluent as compared to the untreated effluent. Treated effluent up to 10% (v/v) concentration reflected low-observed adverse effect levels

  14. Potential of Bambara groundnut (Vigna subterranea (L.) Verdc) milk as a probiotic beverage-a review.

    Science.gov (United States)

    Murevanhema, Yvonne Y; Jideani, Victoria A

    2013-01-01

    Bambara groundnut (Vigna subterraenea (L.) verdc) (BGN) is a legume; its origin have been traced back to Africa, and it is the third important legume; however, it is one of the neglected crops. It is highly nutritious, and has been termed a complete food. Its seed consist of 49%-63.5% carbohydrate, 15%-25% protein, 4.5%-7.4% fat, 5.2%-6.4% fiber, 3.2%-4.4% ash and 2% mineral compared to whole fresh cow milk 88% moisture, 4.8% carbohydrate, 3.2% proteins, 3.4% fat, 0.7% ash, and 0.01% cholesterol. Its chemical composition is comparable to that of soy bean. Furthermore, BGN has been reported to be a potential crop, owing to its nutritional composition, functional properties, antioxidant potential, and a drought resistant crop. Bambara groundnut milk (BGNM) had been rated higher in acceptability than milk from other legumes like soybean and cowpea. Probiotics have been defined as live microorganisms which when administered in adequate amount confer a health benefit on the host. These benefits have been reported to be therapeutic, suppressing the growth and activity in conditions like infectious diarrhea, irritable bowel syndrome, and inflammatory bowel disease. The nutritional profile of BGNM is high enough to sustain the growth of probiotics. BGNs are normally boiled and salted, eaten as a relish or roasted, and eaten as a snack. Hence, BGNM can also be fermented with lactic acid bacteria to make a probiotic beverage that not only increase the economic value of the nutritious legume but also help in addressing malnutrition.

  15. Mobile phone radiation inhibits Vigna radiata (mung bean) root growth by inducing oxidative stress.

    Science.gov (United States)

    Sharma, Ved Parkash; Singh, Harminder Pal; Kohli, Ravinder Kumar; Batish, Daizy Rani

    2009-10-15

    During the last couple of decades, there has been a tremendous increase in the use of cell phones. It has significantly added to the rapidly increasing EMF smog, an unprecedented type of pollution consisting of radiation in the environment, thereby prompting the scientists to study the effects on humans. However, not many studies have been conducted to explore the effects of cell phone EMFr on growth and biochemical changes in plants. We investigated whether EMFr from cell phones inhibit growth of Vigna radiata (mung bean) through induction of conventional stress responses. Effects of cell phone EMFr (power density: 8.55 microW cm(-2); 900 MHz band width; for 1/2, 1, 2, and 4 h) were determined by measuring the generation of reactive oxygen species (ROS) in terms of malondialdehyde and hydrogen peroxide (H(2)O(2)) content, root oxidizability and changes in levels of antioxidant enzymes. Our results showed that cell phone EMFr significantly inhibited the germination (at > or =2 h), and radicle and plumule growths (> or =1 h) in mung bean in a time-dependent manner. Further, cell phone EMFr enhanced MDA content (indicating lipid peroxidation), and increased H(2)O(2) accumulation and root oxidizability in mung bean roots, thereby inducing oxidative stress and cellular damage. In response to EMFr, there was a significant upregulation in the activities of scavenging enzymes, such as superoxide dismutases, ascorbate peroxidases, guaiacol peroxidases, catalases and glutathione reductases, in mung bean roots. The study concluded that cell phone EMFr inhibit root growth of mung bean by inducing ROS-generated oxidative stress despite increased activities of antioxidant enzymes.

  16. Genetic variation for phytic acid content in mungbean(Vigna radiata L. Wilczek)

    Institute of Scientific and Technical Information of China (English)

    Vinod; Janardan; Dhole; Kandali; Srinivasalu; Reddy

    2015-01-01

    Mungbean(Vigna radiata L. Wilczek) is a short-duration legume crop cultivated for seeds that are rich in protein and carbohydrates. Mungbeans contain phytic acid(PA), an anti-nutritional factor that is the main storage form of organic phosphorus in seeds. It is a strong inhibitor against the absorption of nutrients including iron, zinc, calcium and magnesium in monogastric animals. Genotypes with low phytic acid(lpa) in seed may show increased assimilation of nutrients and be useful in breeding lpa cultivars. The present study was conducted to identify lpa sources, genetic variation, heritability, and association with seed coat color, inorganic phosphorus(IP), and seed size in 102 mungbean genotypes including released varieties, land races, mutants, and wild species grown in two seasons: summer 2011 and rabi 2012. PA and IP in dry seeds were estimated by modified colorimetric method and Chen’s modified method,respectively. PA, IP, and 100-seed weight differed significantly in the two seasons. PA content in102 genotypes ranged from 5.74 to 18.98 mg g-1and 5.85 to 20.02 mg g-1in summer 2011 and rabi 2012, respectively. High heritability was found for PA(0.87 and 0.86) and seed size(0.82 and0.83) but low heritability for IP(0.61 and 0.60). A negative correlation was found between PA and seed size(r =-0.183 and-0.267). Yellow and green seed coat genotypes contained significantly less PA than black seed coat genotypes. Cluster analysis revealed the distinctness of wild species, land races and cultivated varieties on the basis of PA content. The genotypes YBSM(6.001 mg g-1) and JL-781(6.179 mg g-1) showed lowest PA. These lpa sources can be used to develop high-yielding mungbean cultivars with low phytic acid.

  17. Genetic diversity of the black gram [Vigna mungo (L.) Hepper] gene pool as revealed by SSR markers.

    Science.gov (United States)

    Kaewwongwal, Anochar; Kongjaimun, Alisa; Somta, Prakit; Chankaew, Sompong; Yimram, Tarikar; Srinives, Peerasak

    2015-03-01

    In this study, 520 cultivated and 14 wild accessions of black gram (Vigna mungo (L.) Hepper) were assessed for diversity using 22 SSR markers. Totally, 199 alleles were detected with a mean of 9.05 alleles per locus. Wild black gram showed higher gene diversity than cultivated black gram. Gene diversity of cultivated accessions among regions was comparable, while allelic richness of South Asia was higher than that of other regions. 78.67% of the wild gene diversity presented in cultivated accessions, indicating that the domestication bottleneck effect in black gram is relatively low. Genetic distance analysis revealed that cultivated black gram was more closely related to wild black gram from South Asia than that from Southeast Asia. STRUCTURE, principal coordinate and neighbor-joining analyses consistently revealed that 534 black gram accessions were grouped into three major subpopulations. The analyses also revealed that cultivated black gram from South Asia was genetically distinct from that from West Asia. Comparison by SSR analysis with other closely related Vigna species, including mungbean, azuki bean, and rice bean, revealed that level of gene diversity of black gram is comparable to that of mungbean and rice bean but lower than that of azuki bean.

  18. Effect of Silver Nanoparticles and Pb(NO32 on the Yield and Chemical Composition of Mung bean (Vigna radiata

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    Saeideh Najafi

    2014-03-01

    Full Text Available Phytotoxic effects of Pb as Pb(NO32 and silver nanoparticles on Mung bean (Vigna radiata planted on contaminated soil was assessed in terms of growth, yield, chlorophyll pigments, phenol and flavonoid content at 120 ppm concentration. Experiments were carried out with 4 treatments in 10 days. Treatments were including (T1 control, (T2 silver nanoparticles (50 ppm, (T3 Pb as Pb (NO32 (120 ppm and (T4 silver nanoparticles (50 ppm plus Pb as Pb(NO32 (120 ppm. Regarding the pigment content, silver nanoparticles-treated plants showed a remarkable increase of chlorophyll. The loss of chlorophyll content was associated with disturbance in photosynthetic capacity which ultimately results in the reduction of Vigna radiate growth. Pb caused a fall in the total content of phenols, while the content of flavonoid not significantly changed. The minimum decrease in root length, weight of root fresh and stem fresh was observed in T4 group, but this factors increased in the other treatments. Also, length of stem and seedling height decreased in control group. Increase length and fresh weight of stem in Pb-treated plants suggest that compatible solutes may contribute to osmotic adjustment at the cellular level and enzyme protection stabilizing the structure of macromolecules and organelles.

  19. Ionizing radiation induced changes in phenotype, photosynthetic pigments and free polyamine levels in Vigna radiata (L.) Wilczek

    International Nuclear Information System (INIS)

    Effects of gamma rays on the free polyamine (PA) levels were studied in Vigna radiata (L.) Wilczek. Seeds exposed to different doses of gamma rays were checked for damage on phenotype, germination frequency and alteration in photosynthetic pigments. Free polyamine levels were estimated from seeds irradiated in dry and water imbibed conditions. Polyamine levels of seedlings grown from irradiated seeds, and irradiated seedlings from unexposed seeds were also measured. Damage caused by gamma irradiation resulted in decrease in final germination percentage and seedling height. Photosynthetic pigments decreased in a dose dependent manner as marker of stress. Polyamines decreased in irradiated dry seeds and in seedlings grown from irradiated seeds. Radiation stress induced increase in free polyamines was seen in irradiated imbibed seeds and irradiated seedlings. Response of polyamines towards gamma rays is dependent on the stage of the life cycle of the plant. - Highlights: ► Gamma irradiation of Vigna radiata (L.) Wilczek seeds and seedlings. ► Decrease in germination frequency. ► Increase in seedling injury with increased dosage of gamma rays. ► Decrease in chlorophyll and carotenoid pigments. ► Change in free polyamine levels

  20. QTL mapping and epistatic interaction analysis in asparagus bean for several characterized and novel horticulturally important traits

    OpenAIRE

    Xu, Pei; Wu, Xiaohua; Wang, Baogen; Hu, Tingting; Lu, Zhongfu; Liu, Yonghua; Qin, Dehui; Wang, Sha; Li, Guojing

    2013-01-01

    Background Asparagus bean (Vigna. unguiculata. ssp sesquipedalis) is a subspecies and special vegetable type of cowpea (Vigna. unguiculata L. Walp.) important in Asia. Genetic basis of horticulturally important traits of asparagus bean is still poorly understood, hindering the utilization of targeted, DNA marker-assisted breeding in this crop. Here we report the identification of quantitative trait loci (QTLs) and epistatic interactions for four horticultural traits, namely, days to first flo...

  1. El obispo Palafox y la cuestión de los ritos chinos en el proceso de extinción de la Compañía de Jesús

    Directory of Open Access Journals (Sweden)

    Eva María ST. CLAIR SEGURADO

    2009-12-01

    Full Text Available RESUMEN: El obispo Palafox y Mendoza experimentó una profunda preocupación por la salud espiritual de los neófitos del Imperio chino, pero conoció la cuestión desde su distante sede diocesana de Puebla de los Angeles. La mentalidad rigorista del prelado, una característica de la herencia tridentina, agravó aún más la enemistad que por razones económicas y jurisdiccionales había surgido entre él y la Compañía de Jesús. Las negativas valoraciones de Palafox acerca de la conducta de los misioneros jesuítas en China, contenidas principalmente en su III Inocenciana, serían recuperadas en el siglo XVIII por aquellos que pretendían hundir a la orden. Las reflexiones de un hombre que tenía abierto un proceso de canonización fueron de gran importancia para personajes como el fiscal Campomanes y los prelados españoles. Es por ello que algunos jesuítas, entre ellos el padre Isla, se esforzaron por restar credibilidad a los testimonios del Venerable.ABSTRACT: The bishop Juan de Palafox y Mendoza was deeply concerned about the spiritual health of the Chinese Empire neophytes, but he became aware of the details of the question from the distance of his diocese in Puebla de los Angeles; his rigorist way of thinking, a characteristic ot Trento Council's inheritance, was an element that bittered even more the animadversion grown between the prelate and the Company of Jesus because of economic and jurisdictional reasons. Palafox's negative conclusions about the behaviour of the Jesuit missionaries in China, basically condensed in his «III Inocenciana», were recaptured in the XVIII century by those who desired the fall of the religious order. The reflections of a man with a canonizing process in course were considerable important for people like the attorney Campomanes and the Spanish bishops, and that's why some Jesuits (Fhater Isla, for instance made big efforts to deny credibility to the Palafox testimonies.

  2. Regulatory considerations surrounding the deployment of Bt-expressing cowpea in Africa: Report of the deliberations of an expert panel

    Science.gov (United States)

    Cowpea (Vigna unguiculata spp. unguiculata) is adapted to the drier agro-ecological zones of West Africa where it is a major source of dietary protein and widely used as a fodder crop. Improving the productivity of cowpea can enhance food availability and security in West Africa. Insect predation -...

  3. Transcriptome sequencing of mung bean (Vigna radiate L. genes and the identification of EST-SSR markers.

    Directory of Open Access Journals (Sweden)

    Honglin Chen

    Full Text Available Mung bean (Vigna radiate (L. Wilczek is an important traditional food legume crop, with high economic and nutritional value. It is widely grown in China and other Asian countries. Despite its importance, genomic information is currently unavailable for this crop plant species or some of its close relatives in the Vigna genus. In this study, more than 103 million high quality cDNA sequence reads were obtained from mung bean using Illumina paired-end sequencing technology. The processed reads were assembled into 48,693 unigenes with an average length of 874 bp. Of these unigenes, 25,820 (53.0% and 23,235 (47.7% showed significant similarity to proteins in the NCBI non-redundant protein and nucleotide sequence databases, respectively. Furthermore, 19,242 (39.5% could be classified into gene ontology categories, 18,316 (37.6% into Swiss-Prot categories and 10,918 (22.4% into KOG database categories (E-value < 1.0E-5. A total of 6,585 (8.3% were mapped onto 244 pathways using the Kyoto Encyclopedia of Genes and Genome (KEGG pathway database. Among the unigenes, 10,053 sequences contained a unique simple sequence repeat (SSR, and 2,303 sequences contained more than one SSR together in the same expressed sequence tag (EST. A total of 13,134 EST-SSRs were identified as potential molecular markers, with mono-nucleotide A/T repeats being the most abundant motif class and G/C repeats being rare. In this SSR analysis, we found five main repeat motifs: AG/CT (30.8%, GAA/TTC (12.6%, AAAT/ATTT (6.8%, AAAAT/ATTTT (6.2% and AAAAAT/ATTTTT (1.9%. A total of 200 SSR loci were randomly selected for validation by PCR amplification as EST-SSR markers. Of these, 66 marker primer pairs produced reproducible amplicons that were polymorphic among 31 mung bean accessions selected from diverse geographical locations. The large number of SSR-containing sequences found in this study will be valuable for the construction of a high-resolution genetic linkage maps, association

  4. Effects of cadmium-induced oxidative stress on growth and nitrogen assimilation in blackgram [Vigna mungo (L. Hepper

    Directory of Open Access Journals (Sweden)

    Mobin Mohammad

    2013-01-01

    Full Text Available Cadmium (Cd accumulation, oxidative damage, and nitrogen metabolism were studied in roots and leaves of 30-d-old blackgram plants [Vigna mungo (L. Hepper], grown in a mixture of soil and compost (3:1 with different Cd concentrations. Significant reductions in both root and shoot dry weight were noted. The concentration of Cd in roots and leaves increased with increasing Cd levels. The level of lipid peroxidation elevated with a consequent increase in H2O2 content under Cd stress in both plant organs. The activity of enzymes mediating the nitrogen assimilation in roots and leaves was greatly reduced in the presence of Cd, except glutamate dehydrogenase (GDH which showed a significant increase.

  5. The in vivo synthesis and accumulation of lectin in developing seeds of black gram (Vigna mungo L. Hepper).

    Science.gov (United States)

    Suseelan, K N; Mitra, R; Bhatia, C R; Gopalakrishna, T

    2004-01-01

    Black gram (Vigna mungo L. Hepper) seed contains two D-galactose-specific lectin species, BGL-I and BGL-II, identified on the basis of elution from ion exchange column and immunochemical cross-reactivity. BGL-I consisted of two monomeric lectins, BGL-I-1 and BGL-1-2, of relative molecular weights 94 and 89 kDa, respectively. BGL-II is another monomeric lectin with a molecular weight of 83 kDa. The in vivo synthesis studies using pulse-chase experiment showed that BGL-II lectin was synthesized as early as 14 days after flowering (DAF). The 94-kDa BGL-I-1 lectin was synthesized around 17 DAF. There was no cotranslational or posttranslational modification of the lectin proteins. The amount of lectin in developing seeds was determined by radial immunodiffusion assay technique. The maximum amount of lectin per seed was found at 28 DAF.

  6. Theoretical model of the three-dimensional structure of a disease resistance gene homolog encoding resistance protein in Vigna mungo.

    Science.gov (United States)

    Basak, Jolly; Bahadur, Ranjit P

    2006-10-01

    Plant disease resistance (R) genes, the key players of innate immunity system in plants encode 'R' proteins. 'R' protein recognizes product of avirulance gene from the pathogen and activate downstream signaling responses leading to disease resistance. No three dimensional (3D) structural information of any 'R' proteins is available as yet. We have reported a 'R' gene homolog, the 'VMYR1', encoding 'R' protein in Vigna mungo. Here, we describe the homology modeling of the 'VMYR1' protein. The model was created by using the 3D structure of an ATP-binding cassette transporter protein from Vibrio cholerae as a template. The strategy for homology modeling was based on the high structural conservation in the superfamily of P-loop containing nucleoside triphosphate hydrolase in which target and template proteins belong. This is the first report of theoretical model structure of any 'R' proteins.

  7. Appearance and Disappearance of Cyanide-Resistant Respiration in Vigna mungo Cotyledons during and following Germination of the Axis.

    Science.gov (United States)

    Morohashi, Y; Matsushima, H

    1983-09-01

    Mitochondrial preparations isolated from black gram (Vigna mungo L.) cotyledons exhibited cyanide-resistant respiration which was of mitochondrial origin. The appearance and the disappearance of this alternative respiration took place during and following imbibition. During the first 6 hours of imbibition, the respiration was completely inhibited by cyanide, but after this time the alternative respiration markedly developed, reaching a maximal cyanide-resistance 12 to 16 hours after the start of imbibition. Subsequently, the alternative respiration gradually disappeared. The actions of cycloheximide and chloramphenicol indicated that the appearance was dependent on cytoplasmic protein synthesis and that the disappearance depended on both cytoplasmic and mitochondrial protein synthesis. The alternative pathway contributed to state 4 respiration, but not to state 3 respiration, in mitochondria from 1-day-old cotyledons. On day 3, it contributed to neither state 3 nor state 4.

  8. COLONIZATION OF VIGNA RADIATA ROOTS BY CHROMIUM RESISTANT BACTERIAL STRAINS OF OCHROBACTRUM INTERMEDIUM, BACILLUS CEREUS AND BREVIBA CTERIUM SP.

    Institute of Scientific and Technical Information of China (English)

    MUHAMMAD Faisal; SHAHIDA Hasnain

    2005-01-01

    The present study deals with colonization potential of plant growth promoting bacterial strains ( Ochrobactrum intermedium, Bacillus cereus and Brevibacterium sp. ) on Vigna radiata roots. The roots were heavily colonized with O. intermedium and B. cereus as compared to Brevibacterium sp. O. intermedium mainly colonized rhizoplane while B. cereus occurred both on the rhizoplane and near root zone. O. intermedium and B. cereus were found to be present both on the rhizoplane and near root zone, while Brevibacterium only in the rhizosphere in the form of groups. The cells of B. cereus were found more in the sites where root exudates were existed. From the above results it was observed that the number of O. intermedium cells were large at root exudate site. Fig 2, Tab 1, Ref 15

  9. Effect of (/sup 60/cobalt) gamma rays on growth and root rot diseases in mungbean (vigna radiata L.)

    International Nuclear Information System (INIS)

    Present investigation showed that gamma rays influences suppressive effect on root rot fungi such as Macrophomina phaseolina (Tassi) Goid, Rhizoctonia solani Kuhn and Fusarium spp., and inducive effect on growth parameters of mung bean (Vigna radiata L.). Seeds of mung bean were treated with gamma rays (/sup 60/Cobalt) at time periods of 0 and 4 minutes and stored for 90 days at room temperature to determine its effect on growth parameters and infection of root infecting fungi. All treatments of gamma rays enhanced the growth parameters as compared to untreated plants. Infection of M. phaseolina, R. solani and Fusarium spp., were significantly decreased on mung bean seeds treated with gamma rays. Gamma rays significantly increased the growth parameters and controlled the root rot fungi up to 90 days of storage of seeds. (author)

  10. Constituents from Vigna vexillata and Their Anti-Inflammatory Activity

    Directory of Open Access Journals (Sweden)

    Guo-Feng Chen

    2012-08-01

    Full Text Available The seeds of Vigna genus are important food resources and there have already been many reports regarding their bioactivities. In our preliminary bioassay, the chloroform layer of methanol extracts of V. vexillata demonstrated significant anti-inflammatory bioactivity. Therefore, the present research is aimed to purify and identify the anti-inflammatory principles of V. vexillata. One new sterol (1 and two new isoflavones (2,3 were reported from the natural sources for the first time and their chemical structures were determined by the spectroscopic and mass spectrometric analyses. In addition, 37 known compounds were identified by comparison of their physical and spectroscopic data with those reported in the literature. Among the isolates, daidzein (23, abscisic acid (25, and quercetin (40 displayed the most significant inhibition of superoxide anion generation and elastase release.

  11. Development of Gene-Based SSR Markers in Rice Bean (Vigna umbellata L. Based on Transcriptome Data.

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    Honglin Chen

    Full Text Available Rice bean (Vigna umbellata (Thunb. Ohwi & Ohashi is a warm season annual legume mainly grown in East Asia. Only scarce genomic resources are currently available for this legume crop species and no simple sequence repeat (SSR markers have been specifically developed for rice bean yet. In this study, approximately 26 million high quality cDNA sequence reads were obtained from rice bean using Illumina paired-end sequencing technology and assembled into 71,929 unigenes with an average length of 986 bp. Of these unigenes, 38,840 (33.2% showed significant similarity to proteins in the NCBI non-redundant protein and nucleotide sequence databases. Furthermore, 30,170 (76.3% could be classified into gene ontology categories, 25,451 (64.4% into Swiss-Prot categories and 21,982 (55.6% into KOG database categories (E-value < 1.0E-5. A total of 9,301 (23.5% were mapped onto 118 pathways using the Kyoto Encyclopedia of Genes and Genome (KEGG pathway database. A total of 3,011 genic SSRs were identified as potential molecular markers. AG/CT (30.3%, AAG/CTT (8.1% and AGAA/TTCT (20.0% are the three main repeat motifs. A total of 300 SSR loci were randomly selected for validation by using PCR amplification. Of these loci, 23 primer pairs were polymorphic among 32 rice bean accessions. A UPGMA dendrogram revealed three major clusters among 32 rice bean accessions. The large number of SSR-containing sequences and genic SSRs in this study will be valuable for the construction of high-resolution genetic linkage maps, association or comparative mapping and genetic analyses of various Vigna species.

  12. Effect of Trichoderma harzianum biomass and Bradyrhizobium sp. strain NC 92 to control leaf blight disease of bambara groundnut (Vigna subterranea) caused by Rhizoctonia solani in the field

    OpenAIRE

    Mana Kanjanamaneesathian; Paranee Sawangsri; Ashara Pengnoo; Jira Suwanprasert

    2007-01-01

    Four hundred and sixty two strains of Trichoderma spp. were isolated from 23 soil samples in which groundnut (Arachis hypogaea L.) and bambara groundnut (Vigna subterranea L.) had been planted in Songkhla, Phattalung, Nakhon Si Thammarat, Narathiwat and Yala provinces. These fungi were tested against Rhizoctonia solani, a causal agent of leaf blight of bambara groundnut, using dual culture technique on PDA medium. Among 462 isolates tested, 226 isolates had an ability to overgrow R. solani co...

  13. Chino Décor Construction Nigeria Ltd.

    Institute of Scientific and Technical Information of China (English)

    You Wanlong

    2007-01-01

    @@ Q: What are the core industries of the investment of your company in Africa? A: In 1989 when I visited Africa for the first time, I went to Nigeria. In the coming decade, I struggled in Africa. I used to run a hotel; following, I struggled in the sphere of investment. Up to now, we have established the market strategy in Africa which bases on Nigeria.

  14. Preliminary Study on Geographical Distribution and Evolutionary Relationships Between Cultivated and Wild Adzuki Bean (Vigna angularis var. angularis and var. nipponensis) by AFLP Analysis

    Institute of Scientific and Technical Information of China (English)

    ZONG Xu-xiao; Duncan Vaughan; Norihiko Tomooka; Akito Kaga; WANG Xin-wang; GUAN Jian-ping; WANG Shu-min

    2003-01-01

    A set of representative 146 adzuki (Vigna angularis var. angularis, and var. nipponensis)germplasm from 6 Asian countries traditionally for adzuki bean production, together with an out group stand-ard rice bean (Vigna umbellata), were analyzed by AFLP methodology using 12 informative primer pairs.313 unambiguous polymorphic bands were created. According to the dendrogram by cluster analysis based onAFLP banding, 143 of the accessions were distinct and revealed enough genetic diversity for identification andclassification of accessions within Vigna angularis. A neighbor joining tree was generated using newly devel-oped Innan's nucleotide diversity estimate from the AFLP data. From analysis, 7 distinct evolutionary groups,named as "Chinese cultivated", "Japanese cultivated", "Japanese complex-Korean cultivated", "Chinesewild", "China Taiwan wild", "Nepal-Bhutan cultivated" and "Hymalayan wild", were detected. Nucleotidediversity with geographical distribution of each group is discussed, regarding the evolutionary relationships be-tween wild and cultivated adzuki beans. The preliminary results indicated that cultivated adzuki bean should bedomesticated from at least 4 progenitors in at least 3 geographical origins.

  15. 多效唑在小豆生产上的应用研究%Study on the Application of PP333 in Vigna angularis Production

    Institute of Scientific and Technical Information of China (English)

    冒宇翔; 汪凯华; 陈惠; 王学军

    2009-01-01

    [目的]研究多效唑在小豆生产上的应用效果.[方法]测定多效唑不同施用剂量、同一施药剂量下不同品种小豆、不同时期施药各处理小豆的性状及产量,并对小豆的性状及产量进行分析,得出多效唑对小豆的综合应用效果.[结果]在一定剂量范围内,多效唑能有效地抑制植株株高,促进分枝,增加节间,提高结荚数;其最佳使用量为1 350 g/hm2,最佳使用期为始蔓期;对早熟直立型品种效果明显优予晚熟蔓生型品种.[结论]多效唑应用在小豆上,能明显改变小豆生长性状,提高产量.%[Objective] Aimed to the application of PP333 in Vigna angularis production.[Method] The application of different doses of PP333, the same dose of application of different varieties of Vigna angularis, the traits and yield of Vigna angularis at different periods of application were determined and analyzed. And the comprehensive application of the effect of Vigna angularis was drawn up.[Result] PP333 could effectively inhibit the plant height, promote branching and increase inter-section to enhance the number of pod in certain dose range.The optimum usage was 1 350 g/hm2 and the optimum period was the period before the spread. Vertical-type varieties of early maturing significantly was superior to the late effects of trailing-type varieties.[Conclusion] PP333 could change growth traits and improve yield of Vigna angularis.

  16. Effects of chemical and biological pesticides on plant growth parameters and rhizospheric bacterial community structure in Vigna radiata

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Sunil; Gupta, Rashi; Sharma, Shilpi, E-mail: shilpi@dbeb.iitd.ac.in

    2015-06-30

    Highlights: • Non-target effects of pesticides employing qualitative and quantitative approaches. • Qualitative shifts in resident and active bacterial community structure. • Abundance of 16S rRNA gene and transcripts were reduced significantly. • Effects of biological pesticide similar to chemical pesticides on rhizospheric bacteria. - Abstract: With increasing application of pesticides in agriculture, their non-target effects on soil microbial communities are critical to soil health maintenance. The present study aimed to evaluate the effects of chemical pesticides (chlorpyrifos and cypermethrin) and a biological pesticide (azadirachtin) on growth parameters and the rhizospheric bacterial community of Vigna radiata. Qualitative and quantitative analysis by PCR-denaturing gradient gel electrophoresis (DGGE) and q-PCR, respectively, of the 16S rRNA gene and transcript were performed to study the impact of these pesticides on the resident and active rhizospheric bacterial community. While plant parameters were not affected significantly by the pesticides, a shift in the bacterial community structure was observed with an adverse effect on the abundance of 16S rRNA gene and transcripts. Chlorpyrifos showed almost complete degradation toward the end of the experiment. These non-target impacts on soil ecosystems and the fact that the effects of the biopesticide mimic those of chemical pesticides raise serious concerns regarding their application in agriculture.

  17. Effects of chemical and biological pesticides on plant growth parameters and rhizospheric bacterial community structure in Vigna radiata.

    Science.gov (United States)

    Singh, Sunil; Gupta, Rashi; Sharma, Shilpi

    2015-06-30

    With increasing application of pesticides in agriculture, their non-target effects on soil microbial communities are critical to soil health maintenance. The present study aimed to evaluate the effects of chemical pesticides (chlorpyrifos and cypermethrin) and a biological pesticide (azadirachtin) on growth parameters and the rhizospheric bacterial community of Vigna radiata. Qualitative and quantitative analysis by PCR-denaturing gradient gel electrophoresis (DGGE) and q-PCR, respectively, of the 16S rRNA gene and transcript were performed to study the impact of these pesticides on the resident and active rhizospheric bacterial community. While plant parameters were not affected significantly by the pesticides, a shift in the bacterial community structure was observed with an adverse effect on the abundance of 16S rRNA gene and transcripts. Chlorpyrifos showed almost complete degradation toward the end of the experiment. These non-target impacts on soil ecosystems and the fact that the effects of the biopesticide mimic those of chemical pesticides raise serious concerns regarding their application in agriculture. PMID:25791643

  18. Nontarget effects of chemical pesticides and biological pesticide on rhizospheric microbial community structure and function in Vigna radiata.

    Science.gov (United States)

    Singh, Sunil; Gupta, Rashi; Kumari, Madhu; Sharma, Shilpi

    2015-08-01

    Intensive agriculture has resulted in an indiscriminate use of pesticides, which demands in-depth analysis of their impact on indigenous rhizospheric microbial community structure and function. Hence, the objective of the present work was to study the impact of two chemical pesticides (chlorpyrifos and cypermethrin) and one biological pesticide (azadirachtin) at two dosages on the microbial community structure using cultivation-dependent approach and on rhizospheric bacterial communities involved in nitrogen cycle in Vigna radiata rhizosphere through cultivation-independent technique of real-time PCR. Cultivation-dependent study highlighted the adverse effects of both chemical pesticide and biopesticide on rhizospheric bacterial and fungal communities at different plant growth stages. Also, an adverse effect on number of genes and transcripts of nifH (nitrogen fixation); amoA (nitrification); and narG, nirK, and nirS (denitrification) was observed. The results from the present study highlighted two points, firstly that nontarget effects of pesticides are significantly detrimental to soil microflora, and despite being of biological origin, azadirachtin exerted negative impact on rhizospheric microbial community of V. radiata behaving similar to chemical pesticides. Hence, such nontarget effects of chemical pesticide and biopesticide in plants' rhizosphere, which bring out the larger picture in terms of their ecotoxicological effect, demand a proper risk assessment before application of pesticides as agricultural amendments. PMID:25801369

  19. RESIDU PESTISIDA PADA SAYURAN KUBIS (Brassica oleracea L. DAN KACANG PANJANG ( Vigna sinensis L. YANG DIPASARKAN DI PASAR BADUNG DENPASAR

    Directory of Open Access Journals (Sweden)

    K Agung Sudewa

    2012-11-01

    Full Text Available Pesticides residue of organophosphate and carbamate i.e. diazinon, chlorpyriphos, fentoate, carbaril and BPMC were tested on cabbage (Brassica oleracea L. and long bean (Vigna sinensis L.. The purpose of this study was to know the level of pesticides residue remaining on cabbage and long bean marketed in Badung Market, Denpasar.The samples were determined proportionally based on purposive sampling method. The proportion of sample was 10% of the total cabbage and snake bean sold in Badung market.Result of present study showed that residue of insecticides such as diazinon, chlorpyriphos, fentoate, carbaril, and BPMC remaining on the head of cabbage and snake bean marketed in Badung market was affected by the frequencies of their use in the field, in which chlorpyriphos was used by 60-65% of the farmers and carbaril by 40% of the farmers. Their residues on cabbage anf snake bean were 0.525 ppm and 1.296 ppm for chlorpyriphos (organophosphate; 0.303 ppm and 0.471 ppm for carbaril (carbamate. These result suggested that residue of chlorpyriphos on cabbage and snake bean were higher than MRL (Maximum Residue Limit for vegetable crops, i.e. 0.5 ppm.

  20. Effects of chemical and biological pesticides on plant growth parameters and rhizospheric bacterial community structure in Vigna radiata

    International Nuclear Information System (INIS)

    Highlights: • Non-target effects of pesticides employing qualitative and quantitative approaches. • Qualitative shifts in resident and active bacterial community structure. • Abundance of 16S rRNA gene and transcripts were reduced significantly. • Effects of biological pesticide similar to chemical pesticides on rhizospheric bacteria. - Abstract: With increasing application of pesticides in agriculture, their non-target effects on soil microbial communities are critical to soil health maintenance. The present study aimed to evaluate the effects of chemical pesticides (chlorpyrifos and cypermethrin) and a biological pesticide (azadirachtin) on growth parameters and the rhizospheric bacterial community of Vigna radiata. Qualitative and quantitative analysis by PCR-denaturing gradient gel electrophoresis (DGGE) and q-PCR, respectively, of the 16S rRNA gene and transcript were performed to study the impact of these pesticides on the resident and active rhizospheric bacterial community. While plant parameters were not affected significantly by the pesticides, a shift in the bacterial community structure was observed with an adverse effect on the abundance of 16S rRNA gene and transcripts. Chlorpyrifos showed almost complete degradation toward the end of the experiment. These non-target impacts on soil ecosystems and the fact that the effects of the biopesticide mimic those of chemical pesticides raise serious concerns regarding their application in agriculture

  1. A deep sequencing analysis of transcriptomes and the development of EST-SSR markers in mungbean (Vigna radiata)

    Indian Academy of Sciences (India)

    CHANGYOU LIU; BAOJIE FAN; ZHIMIN CAO; QIUZHU SU; YAN WANG; ZHIXIAO ZHANG; JING WU; JING TIAN

    2016-09-01

    Mungbean (Vigna radiata L. Wilczek) is one of the most important leguminous food crops in Asia. We employed Illumina paired-end sequencing to analyse transcriptomes of three different mungbean genotypes. A total of 38.3–39.8 million paired-end reads with 73 bp lengths were generated. The pooled reads from the three libraries were assembled into 56,471 transcripts. Following a cluster analysis, 43,293 unigenes were obtained with an average length of 739 bp and N50 length of 1176 bp. Of the unigenes, 34,903 (80.6%) had significant similarity to known proteins in the NCBI nonredundant protein database (Nr), while 21,450 (58.4%) had BLAST hits in the Swiss-Prot database (E-value < 10⁻⁵). Further, 1245 differential expression genes were detected among three mungbean genotypes. In addition, we identified 3788 expressed sequence tag-simple sequence repeat (EST-SSR) motifs that could be used as potential molecular markers. Among 320 tested loci, 310 (96.5%) yielded amplification products, and 151 (47.0%) exhibited polymorphisms among six mungbean accessions. These transcriptome data and mungbean EST-SSRs could serve as a valuable resource for novel gene discovery and the marker-assisted selective breeding of this specie

  2. In Vivo Synthesis and Turnover of α-Amylase in Attached and Detached Cotyledons of Vigna mungo Seeds 1

    Science.gov (United States)

    Koshiba, Tomokazu; Minamikawa, Takao

    1983-01-01

    α-Amylase activity increased in attached cotyledons of germinated Vigna mungo seeds until the 5th day after imbibition and decreased thereafter, whereas in detached and incubated cotyledons the activity continuously increased and, at the 6th day, reached the value more than three times that of the maximum activity of attached cotyledons. Zymograms of the activities and Ouchterlony double immunodiffusion test on the activities of attached and detached cotyledons showed that the increase of activity in detached cotyledons was due to the identical enzyme as in attached tissues. α-Amylase contents, determined by single radial immunodiffusion method, changed in parallel with enzyme activity in both attached and detached cotyledons, which also suggested the de novo synthesis of α-amylase in V. mungo cotyledons. The rate of incorporation of the label from [3H]leucine into α-amylase and the ratios of dpm in α-amylase/dpm in trichloroacetic acid-insoluble fraction did not show significant difference between attached and detached cotyledons. The results indicated that in attached cotyledons fluctuation of α-amylase activity was regulated by both synthesis and degradation of the enzyme, whereas in detached cotyledons α-amylase was synthesized and accumulated, because of low degrading activity during incubation. Images Fig. 1 Fig. 2 Fig. 4 PMID:16662780

  3. Correlation of endogenous free polyamine levels with root nodule senescence in different genotypes in Vigna mungo L.

    Science.gov (United States)

    Lahiri, Kajari; Chattopadhyay, Soumen; Ghosh, Bharati

    2004-05-01

    Endogenous free polyamines, nitrogenase (EC 1.1.8.6.1, acetylene reduction), and leghaemoglobin (pyridine-hemochrome assay) levels were compared among five genotypes of developing Vigna root nodules grown under field conditions. Nitrogenase activity and leghaemoglobin level attained a peak at the flowering stage and gradually declined thereafter. Individual and total polyamine also followed the same pattern. Ranking on the basis of legume yield and other morphometric attributes was PDU-2 > UH-28 > UH-82 > T-9 > Sardhomash. Except spermine, the levels of putrescine, spermidine, and total polyamine showed significant differences (p<0.05) amongst the genotypes, particularly from flowering to mid-pod development stage. Genotype, development stage, and their interaction between the two had significant (p<0.01) effects on individual as well as total polyamines. Moreover, significant high linear correlations were found between total free polyamine and putrescine with conventional nodule senescence marker like nitrogenase (R2 = 0.94 and R2 = 0.92, respectively). Putrescine had an overall positive correlation with high legume yield. The results strongly suggest a relationship between polyamine and nodule senescence. Endogenous free polyamine and putrescine may be considered as genotypic markers for nodule senescence in field grown V. mungo. It is suggested that the flowering stage is more suitable for selection.

  4. Identification and expression profiling of Vigna mungo microRNAs from leaf small RNA transcriptome by deep sequencing.

    Science.gov (United States)

    Paul, Sujay; Kundu, Anirban; Pal, Amita

    2014-01-01

    MicroRNAs (miRNAs) represent a class of small non-coding RNA molecules that play a crucial role in post-transcriptional gene regulation. Several conserved and species-specific miRNAs have been characterized to date, predominantly from the plant species whose genome is well characterized. However, information on the variability of these regulatory RNAs in economically important but genetically less characterized crop species are limited. Vigna mungo is an important grain legume, which is grown primarily for its protein-rich edible seeds. miRNAs from this species have not been identified to date due to lack of genome sequence information. To identify miRNAs from V. mungo, a small RNA library was constructed from young leaves. High-throughput Illumina sequencing technology and bioinformatic analysis of the small RNA reads led to the identification of 66 miRNA loci represented by 45 conserved miRNAs belonging to 19 families and eight non-conserved miRNAs belonging to seven families. Besides, 13 novel miRNA candidates in V. mungo were also identified. Expression patterns of selected conserved, non-conserved, and novel miRNA candidates have been demonstrated in leaf, stem, and root tissues by quantitative polymerase chain reaction, and potential target genes were predicted for most of the conserved miRNAs. This information offers genomic resources for better understanding of miRNA mediated post-transcriptional gene regulation.

  5. Effect of cooking and supplementation with different kinds of meats on the nutritional value of mash (Vigna mungo).

    Science.gov (United States)

    Bhatty, N; Gilani, A H; Nagra, S A

    2000-05-01

    The study was conducted to determine the nutritional value of mash (Vigna mungo) in raw and cooked forms and as effected by supplementation with different kind of meat, i.e. poultry, mutton and beef at 10, 15 and 20% levels. Nutritional assessment of all mash-containing diets (without or with supplementation) was made by chemical analysis as well as through rat assay. Mash contained 23.83% protein. Cooking resulted in minor changes in nutrients. Mash had 1.79% lysine which was reduced by 35% on cooking. All other amino acids also showed losses during cooking. Protein efficiency ratio (PER) of diet containing raw mash was 1.9% and cooking improved it to 2.8%. True digestibility (TD) also showed a significant improvement. Supplementation of mash with different kinds of meat did not improve the PER significantly over unsupplemented diet containing cooked mash only. TD, however, was improved from 74.89% in cooked to 75.58-87.06% in supplemented diets. Similarly net protein utilization (NPU), as a result of meat supplementation, improved from 43.54% in cooked to 42.88%-51.96%. Higher PER, TD and NPU values were observed in diets containing mash supplemented with 20% level of different meats.

  6. In Vivo Synthesis and Turnover of alpha-Amylase in Attached and Detached Cotyledons of Vigna mungo Seeds.

    Science.gov (United States)

    Koshiba, T; Minamikawa, T

    1983-01-01

    alpha-Amylase activity increased in attached cotyledons of germinated Vigna mungo seeds until the 5th day after imbibition and decreased thereafter, whereas in detached and incubated cotyledons the activity continuously increased and, at the 6th day, reached the value more than three times that of the maximum activity of attached cotyledons. Zymograms of the activities and Ouchterlony double immunodiffusion test on the activities of attached and detached cotyledons showed that the increase of activity in detached cotyledons was due to the identical enzyme as in attached tissues. alpha-Amylase contents, determined by single radial immunodiffusion method, changed in parallel with enzyme activity in both attached and detached cotyledons, which also suggested the de novo synthesis of alpha-amylase in V. mungo cotyledons.The rate of incorporation of the label from [(3)H]leucine into alpha-amylase and the ratios of dpm in alpha-amylase/dpm in trichloroacetic acid-insoluble fraction did not show significant difference between attached and detached cotyledons. The results indicated that in attached cotyledons fluctuation of alpha-amylase activity was regulated by both synthesis and degradation of the enzyme, whereas in detached cotyledons alpha-amylase was synthesized and accumulated, because of low degrading activity during incubation.

  7. Genetic variability studies for yield and its component traits in RIL population of blackgram (Vigna mungo (L. Hepper

    Directory of Open Access Journals (Sweden)

    Sowmini.K and Jayamani. P

    2013-03-01

    Full Text Available Blackgram or Urdbean, being a fourth important pulse crop in India has low genetic variability, low harvest index and nosignificant improvement in its productivity till date. An inter sub specific mapping population was developed by crossingVBN(Bg 5 x Vigna mungo var. silvestris 22/10 by single seed decent method. The genetic variability parameters werestudied in a RIL (Recombinant Inbred Line population consisting of 193 lines and their parents. The higher estimates ofPCV were observed for all the traits when compared with GCV. However, GCV was found to be high for the traits singleplant yield, number of clusters per plant and number of pods per plant. High heritability per cent was observed for days tomaturity, number of seeds per pod and hundred seed weight. High genetic advance as per cent of mean was observed forplant height, number of clusters per plant, number of pods per plant, single plant yield and hundred seed weight. Highheritability coupled with high genetic advance as per cent of mean was observed for hundred seed weight. Transgressivesegregants were observed for all the traits. These could be used further for yield testing apart from utilizing it as prebreeding material. The mapping population could be used for mapping of genes for important traits.

  8. Multiple abiotic stress tolerance in Vigna mungo is altered by overexpression of ALDRXV4 gene via reactive carbonyl detoxification.

    Science.gov (United States)

    Singh, Preeti; Kumar, Deepak; Sarin, Neera Bhalla

    2016-06-01

    Vigna mungo (blackgram) is an important leguminous pulse crop, which is grown for its protein rich edible seeds. Drought and salinity are the major abiotic stresses which adversely affect the growth and productivity of crop plants including blackgram. The ALDRXV4 belongs to the aldo-keto reductase superfamily of enzymes that catalyze the reduction of carbonyl metabolites in the cells and plays an important role in the osmoprotection and detoxification of the reactive carbonyl species. In the present study, we developed transgenic plants of V. mungo using Agrobacterium mediated transformation. The transgene integration was confirmed by Southern blot analysis whereas the expression was confirmed by RT-PCR, Western blot and enzyme activity. The T1 generation transgenic plants displayed improved tolerance to various environmental stresses, including drought, salt, methyl viologen and H2O2 induced oxidative stress. The increased aldose reductase activity, higher sorbitol content and less accumulation of the toxic metabolite, methylglyoxal in the transgenic lines under non-stress and stress (drought and salinity) conditions resulted in increased protection through maintenance of better photosynthetic efficiency, higher relative water content and less photooxidative damage. The accumulation of reactive oxygen species was remarkably decreased in the transgenic lines as compared with the wild type plants. This study of engineering multiple stress tolerance in blackgram, is the first report to date and this strategy for trait improvement is proposed to provide a novel germplasm for blackgram production on marginal lands.

  9. Construction of a genetic linkage map of black gram, Vigna mungo (L.) Hepper, based on molecular markers and comparative studies.

    Science.gov (United States)

    Gupta, S K; Souframanien, J; Gopalakrishna, T

    2008-08-01

    A genetic linkage map of black gram, Vigna mungo (L.) Hepper, was constructed with 428 molecular markers using an F9 recombinant inbred population of 104 individuals. The population was derived from an inter-subspecific cross between a black gram cultivar, TU94-2, and a wild genotype, V. mungo var. silvestris. The linkage analysis at a LOD score of 5.0 distributed all 428 markers (254 AFLP, 47 SSR, 86 RAPD, and 41 ISSR) into 11 linkage groups. The map spanned a total distance of 865.1 cM with an average marker density of 2 cM. The largest linkage group spanned 115 cM and the smallest linkage group was of 44.9 cM. The number of markers per linkage group ranged from 11 to 86 and the average distance between markers varied from 1.1 to 5.6 cM. Comparison of the map with other published azuki bean and black gram maps showed high colinearity of markers, with some inversions. The current map is the most saturated map for black gram to date and will provide a useful tool for identification of QTLs and for marker-assisted selection of agronomically important characters in black gram.

  10. Identification and expression profiling of Vigna mungo microRNAs from leaf small RNA transcriptome by deep sequencing

    Institute of Scientific and Technical Information of China (English)

    Sujay Paul; Anirban Kundu; Amita Pal

    2014-01-01

    MicroRNAs (miRNAs) represent a class of small non-coding RNA molecules that play a crucial role in post-transcriptional gene regulation. Several conserved and species-specific miRNAs have been characterized to date, predominantly from the plant species whose genome is well characterized. However, information on the variability of these regulatory RNAs in economically important but genetically less characterized crop species are limited. Vigna mungo is an important grain legume, which is grown primarily for its protein-rich edible seeds. miRNAs from this species have not been identified to date due to lack of genome sequence information. To identify miRNAs from V. mungo, a small RNA library was constructed from young leaves. High-throughput Illumina sequencing technology and bioinformat-ic analysis of the small RNA reads led to the identification of 66 miRNA loci represented by 45 conserved miRNAs belonging to 19 families and eight non-conserved miRNAs belonging to seven families. Besides, 13 novel miRNA candidates in V. mungo were also identified. Expression patterns of selected conserved, non-conserved, and novel miRNA candidates have been demonstrated in leaf, stem, and root tissues by quantitative polymerase chain reaction, and potential target genes were predicted for most of the conserved miRNAs. This information offers genomic resour-ces for better understanding of miRNA mediated post-transcriptional gene regulation.

  11. Ameliorative potential of Vigna mungo seeds on hyperglycemia mediated oxidative stress and hyperlipidemia in STZ diabetic rats

    Directory of Open Access Journals (Sweden)

    Jangra Meenu

    2011-01-01

    Full Text Available Ethnomedicinally the seeds of Vigna mungo are used as cooling astringent, diet during fever, poultice for abscesses, soap alternative. The increased oxidative stress plays an important role in the etiology and pathogenesis of diabetes mellitus and its various complications. This study was designed to examine the effect of hydroalcoholic extract (VME of V. mungo seeds on STZ-diabetic rats by measuring glycemia, lipid profile, and lipid peroxidation parameters (MDA, PCO, and GSH and antioxidant enzymes activities (SOD, CAT, and GPx. The levels of glucose, TG, TC, MDA, and PCO were increased significantly whereas the levels of serum insulin, reduced glutathione (GSH, superoxide dismutase (SOD, catalase (CAT and Glutathione peroxidise (GPx were decreased in STZ induced diabetic rats. Administration of VME (200 mg/kg bw and 400 mg/kg bw p.o. to diabetic rats for 28 days showed a significant decrease in serum glucose, TG, TC, MDA, and PCO. In addition, we also summarize here that the levels of serum insulin, superoxide dismutase, catalase, GPx, and reduced glutathione (GSH were increased in VME treated diabetic rats. The antioxidant and antihyperglycemic effect of VME was compared with glibenclamide, a well-known antioxidant and antihyperglycemic drug. The findings in this study suggest that the VME possesses a significant favourable effect on antioxidant defense system in addition to its antidiabetic effect. Nonetheless, this study provides evidence that could help explain how the traditional use of V. mungo has been successful in the treatment of various disorders in humans.

  12. Synergism of VAM and Rhizobium on production and metabolism of IAA in roots and root nodules of Vigna mungo.

    Science.gov (United States)

    Chakrabarti, Jayanta; Chatterjee, Sabyasachi; Ghosh, Sisir; Chatterjee, Narayan Chandra; Dutta, Sikha

    2010-09-01

    Mature and healthy root nodules of Vigna mungo appeared to contain higher amount of indole-acetic acid (IAA) than non-nodulated roots. Dual effect of VAM fungus, Glomus fasciculatum and the nitrogen-fixing bacteria, Rhizobium sp. on the nodulation of roots of V. mungo was studied. It was recorded that the roots which were inoculated simultaneously with both the symbionts i.e., G. fasciculatum and Rhizobium exhibited greater amount of IAA production than the non-inoculated roots. A tryptophan pool present in the mature nodules and young leaves might serve as a precursor for IAA production in the roots and in the nodules. Activity of IAA-metabolizing enzymes, such as IAA oxidase, peroxidase, and polyphenol oxidase was investigated which indicates the active metabolism of IAA in roots and nodules. The Rhizobium symbiont isolated from fresh nodules of V. mungo produced significant amount of IAA under in vitro condition when tryptophan was added to the medium as precursor. Present study represents some beneficial effects of Rhizobium and G. fasciculatum on the production and metabolism of IAA in roots and nodules of V. mungo. The important physiological implication of the study on IAA production and its metabolism in Rhizobium-Legume-VAM tripartite symbiosis is certainly representing a new approach to satisfy the hormonal balance in the host plant.

  13. Isolation of a putative receptor for KDEL-tailed cysteine proteinase (SH-EP) from cotyledons of Vigna mungo seedlings.

    Science.gov (United States)

    Tsuru-Furuno, A; Okamoto, T; Minamikawa, T

    2001-10-01

    SH-EP is the major papain-type proteinase expressed in cotyledons of germinated Vigna mungo seeds. The proteinase possesses a KDEL sequence at the C-terminus although the mature form of SH-EP is localized in vacuoles. It has also been shown that the proform of SH-EP is accumulated at the edge or middle region of the endoplasmic reticulum, and the accumulated proSH-EP is directly transported to vacuoles via the KDEL-tailed cysteine proteinase-accumulating vesicle, KV. In this study, to address the transport machinery of proSH-EP through KV, putative receptor for proSH-EP was isolated from membrane proteins of cotyledons of V. mungo seedlings using a proSH-EP-immobilized column. The deduced amino acid sequence from cDNA to the protein revealed that the putative receptor for proSH-EP is a member of vacuolar sorting receptor, VSR, that is known to be localized in the Golgi-complex and/or clathrin coated vesicle. We carried out subcellular fractionation of cotyledon cells and subsequently conducted SDS-PAGE/immunoblotting and immunocytochemistry with anti-V. mungo VSR (VmVSR) or SH-EP antibody. The results showed that VmVSR is co-localized in the fraction of the gradient in which KV existed.

  14. Assessment of microbial communities in mung bean (Vigna radiata) rhizosphere upon exposure to phytotoxic levels of Copper.

    Science.gov (United States)

    Sharaff, Murali; Archana, G

    2015-11-01

    Pollution of agricultural soils by Cu is of concern as it could bring about alterations in microbial communities, ultimately eliminating certain plant beneficial bacteria thus disturbing soil fertility and plant growth. To understand the response of rhizobacterial communities upon Cu perturbation, mung bean (Vigna radiata) plants were grown in agricultural soil amended with CuSO4 (0-1000 mg kg(-1) ) under laboratory conditions. Culture-independent and -dependent Denaturing Gradient Gel Electrophoresis (CI-DGGE and CD-DGGE) fingerprinting techniques were employed to monitor rhizobacterial community shifts upon Cu amendment. In group specific PCR-DGGE, a negative impact was seen on α-Proteobacteria followed by β-Proteobacteria resulting in a concomitant decrease in diversity indices with increased Cu concentration. No significant changes were observed in Firmicutes and Actinomycetes populations. In CD-DGGE rhizobacterial community shift was observed above 500 mg kg(-1) (CuSO4 ), however certain bands were predominantly present in all treatments. Plants showed toxic effects by reduction in growth and elevated Cu accumulation, with root system being affected prominently. From this study it is evident that above 250 mg kg(-1) , rhizobacterial communities are adversely affected. α-Proteobacteria was found to be a sensitive bio-indicator for Cu toxicity and is of particular significance since this group includes majority of plant growth promoting rhizobacteria. PMID:26073767

  15. Effect of Mobile Phone Radiations on Morphological and Biochemical Parameters of Mung Bean (Vigna radiata and Wheat (Triticum aestivum Seedlings

    Directory of Open Access Journals (Sweden)

    Mobin Afzal

    2012-03-01

    Full Text Available Over the last two decades an enormous escalation in wireless equipments has been made our lives easy. The uses of wireless accessories including mobile phone have been enabled instant our communication resources advance and fast, on the other hand along with their advantages there is great increase in radiations, an unprecedented type of pollution, in our environment. Current study was an attempt to observe the morphological and biochemical changes induced by cell phone radiations on Mung bean (Vigna radiata and Wheat (Triticum aestivum seedlings. The effect of cell phone Electromagnetic Radiations (EMR were determined by measuring membrane damage in terms of melondialdehyde [MDA] contents and changes in the levels of antioxidant enzymes like Guaiacol Peroxidase (GPX, Ascorbate Peroxidase (APX, and Catalase (CAT. Our results showed that cell phone EMR caused significant reduction in growth, fresh weight, dry weight, and relative water contents. Where as MDA contents and antioxidant enzymes were increased in stressed seedlings as compared to unstressed seedlings. We concluded that radiations emitted by mobile phone can induce oxidative stress which results in reduced growth and increase in the activity of antioxidant enzymes in mung bean and wheat seedlings.

  16. Assessment of microbial communities in mung bean (Vigna radiata) rhizosphere upon exposure to phytotoxic levels of Copper.

    Science.gov (United States)

    Sharaff, Murali; Archana, G

    2015-11-01

    Pollution of agricultural soils by Cu is of concern as it could bring about alterations in microbial communities, ultimately eliminating certain plant beneficial bacteria thus disturbing soil fertility and plant growth. To understand the response of rhizobacterial communities upon Cu perturbation, mung bean (Vigna radiata) plants were grown in agricultural soil amended with CuSO4 (0-1000 mg kg(-1) ) under laboratory conditions. Culture-independent and -dependent Denaturing Gradient Gel Electrophoresis (CI-DGGE and CD-DGGE) fingerprinting techniques were employed to monitor rhizobacterial community shifts upon Cu amendment. In group specific PCR-DGGE, a negative impact was seen on α-Proteobacteria followed by β-Proteobacteria resulting in a concomitant decrease in diversity indices with increased Cu concentration. No significant changes were observed in Firmicutes and Actinomycetes populations. In CD-DGGE rhizobacterial community shift was observed above 500 mg kg(-1) (CuSO4 ), however certain bands were predominantly present in all treatments. Plants showed toxic effects by reduction in growth and elevated Cu accumulation, with root system being affected prominently. From this study it is evident that above 250 mg kg(-1) , rhizobacterial communities are adversely affected. α-Proteobacteria was found to be a sensitive bio-indicator for Cu toxicity and is of particular significance since this group includes majority of plant growth promoting rhizobacteria.

  17. Experimental effects of sand-dust storm on tolerance index, percentage phototoxicity and chlorophyll a fluorescence of Vigna radiata L.

    Directory of Open Access Journals (Sweden)

    M. Alavi

    2015-03-01

    Full Text Available In arid and semi-arid parts of the world excessive mineral aerosol carried by air parcels is a common climatic incident with well-known environmental side effects. In this way, we studied the role of sand-dust accumulation on various aspects of productivity of Vigna radiata L. including dry mass (DM, chlorophyll (Chl a, b, Chlorophyll a fluorescence (effective quantum yield of PSII photochemistry (ФPSII, maximal quantum yield of PSII photochemistry (Fv/Fm and electron transport rate (ETR. V. radiata was exposed to a gradient of dust concentrations in a dust chamber (0.5 (T1, 1(T2 and 1.5 g/m3 (T3 simulated by a dust generator for a period of 60 days. Results of this experiment indicate that DM and Chl content of shoot are negatively correlated with the intensity of the dust exposure. Exposure of V. radiata to dust compared with the control was caused 5% (T1, 14% (T2 and 27% (T3 reduction in leaf DM (p≤0.05, ANOVA. Also, exposure to the dust induced a significant (p≤0.05 reduction in the Total Chl content in (T3 25%. Also, we showed that ФPSII, ETR and Fv/Fm were affected by increasing of the dust concentrations. Exposure to the dust resulted in a significant reduction in ETR of 15%, 22%, and 43%.

  18. Effect of fermentation with Monascus pilosus on the antioxidant activities and phenolic acid contents of adzuki bean (Vigna angularis)

    Institute of Scientific and Technical Information of China (English)

    Jinhua Cheng; Sung-Kwon Lee; Joo-Won Suh; Seung HwanYang

    2015-01-01

    Objective: To enhance physiological activities of adzuki bean (Vigna angularis) via fermentation with Monascus pilosus (M. pilosus). Methods: The adzuki bean fermentation conditions with M. pilosus were optimized, and the effect of Monascus-fermentation on the antioxidant capacity and phenolic acid contents of adzuki bean was investigated. Results: Optimal fermentation conditions were determined by the production of monacolin K. The highest monacolin K production was observed in 5% inoculum sized on day 15 in fermentation. Free and bound phenolic acids were isolated from native and fermented adzuki bean. A 1.9-fold decrease was observed in bound p-coumaric acid content, whereas the contents of bound ferulic and sinapic acids were increased by 28- and 1.7-fold, respectively. However, the contents of free phenolic acids such as p-coumaric, ferulic, and sinapic acids were increased by 2.6-, 5.2-, and 7.2-fold, respectively. The fermentation of adzuki bean by M. pilosus enhanced the activities of DPPH· radical scavenging, ferrous ion-chelating, nitric oxide scavenging, and ferric antioxidant reducing activities 2.2-, 1.7-, 1.2-, and 1.8-fold, respectively. Conclusions: Results from our study suggest that the contents of p-coumaric, ferulic, and sinapic acids in adzuki bean were highly increased by fermentation with M. pilosus, resulting in enhanced various antioxidant activities.

  19. Identification and expression profiling of Vigna mungo microRNAs from leaf small RNA transcriptome by deep sequencing.

    Science.gov (United States)

    Paul, Sujay; Kundu, Anirban; Pal, Amita

    2014-01-01

    MicroRNAs (miRNAs) represent a class of small non-coding RNA molecules that play a crucial role in post-transcriptional gene regulation. Several conserved and species-specific miRNAs have been characterized to date, predominantly from the plant species whose genome is well characterized. However, information on the variability of these regulatory RNAs in economically important but genetically less characterized crop species are limited. Vigna mungo is an important grain legume, which is grown primarily for its protein-rich edible seeds. miRNAs from this species have not been identified to date due to lack of genome sequence information. To identify miRNAs from V. mungo, a small RNA library was constructed from young leaves. High-throughput Illumina sequencing technology and bioinformatic analysis of the small RNA reads led to the identification of 66 miRNA loci represented by 45 conserved miRNAs belonging to 19 families and eight non-conserved miRNAs belonging to seven families. Besides, 13 novel miRNA candidates in V. mungo were also identified. Expression patterns of selected conserved, non-conserved, and novel miRNA candidates have been demonstrated in leaf, stem, and root tissues by quantitative polymerase chain reaction, and potential target genes were predicted for most of the conserved miRNAs. This information offers genomic resources for better understanding of miRNA mediated post-transcriptional gene regulation. PMID:24138283

  20. Influencia de la Tecnología Flat Deck de origen Chino y Español en el Comportamiento Productivo y de Salud en precedas - Influence of Technology Flat Deck Chinese and Spanish origin in the Production and Health Behavior in Pre-Fatten

    Directory of Open Access Journals (Sweden)

    Hernández Crus Yunier

    2010-07-01

    Full Text Available ResumenInfluencia de la Tecnología Flat Deck de origen Chino y Español en el Comportamiento Productivo y de Salud en precebas. http:// Con el objetivo Evaluar el comportamiento productivo y de salud de cerdos de la categoría precebas alojadas en Tecnología Flat Deck de origen Chino y Español. Se utilizaron 80 grupos tecnológicos de cerdosmestizos (Hembras- Yorkland y Machos CC-21, L-35 y Duroc de launidad de producción de pulmones útiles “Julio Antonio Mella”, sedividieron en dos grupos experimentales de 40 grupos tecnológicos porcada tecnología, distribuidos al azar, con 1458 animales para latecnología española y 1807 animales para la tecnología china losdatos fueron tomados de la tarjetas que acompaña a los animalesdurante la etapa, hasta el momento del sacrificio, fueron distribuidos 9 animales por bóxer, durante 49 días, sacrificados entre 26 y 30 Kg de peso vivo y 75 días de vida. Los indicadores productivos no mostraron diferencias significativas (p ≥ 0.05 entre los cerdos de ambas tecnologías. Los valores en la GMD fueron para la tecnología de origen español de 422 g y para la china de 414 g, siendo el peso final similar de 27.7 g y 27.2 g para ambas tecnologías respectivamente. Los resultados obtenidos en la Conversión alimenticia no fueron significativos (p> 0.05 entre las tecnologías, los indicadores de salud mostraron diferencias significativas (p 0.05 between technologies, health indicators showed significant differences (p <0.01, with a greater number of sick and dead animals with 370 and 127 in home technology respectively and 257 Chinese and 78 Spanish. Flat Deck technology offers similar results Spanish origin productive technology of Chinese origin, not so for health indicators.

  1. 豇豆种子耐贮性的三种人工老化方法的比较研究%Comparative Study on Three Artificial Aging Methods in Evaluating the Storability of Common Cowpea [Vigna unguiculata (Linn.)Walp. ]Seeds

    Institute of Scientific and Technical Information of China (English)

    陈晓玲; 卢新雄; 陈叔平

    2001-01-01

    2个豇豆品种经40℃、100%RH老化处理后,种子的发芽势、发芽率、发芽指数和活力指数等的变化趋势均与自然老化种子的死亡规律相似,呈近似反S型曲线;而用58±1℃热水和50%甲醇溶液老化处理后,2个品种的表现与自然老化种子的不同。因此,40℃、100%RH高温高湿适用于评价豇豆种子耐贮性的人工老化,其最适老化时间为5~6d;适宜的生理生化指标为电导率。

  2. 氯化钠胁迫与种子大小及其互作对架豆角种子萌发及幼苗生长的影响%Effect of NaCl Stress,Seed Size and Their Interaction on Seed Germination and Seedling Growth of Vigna unguiculata (Linn.) Walp.

    Institute of Scientific and Technical Information of China (English)

    金正律; 刘聪聪

    2013-01-01

    以“架豆王”架豆角为试材,研究了NaCl胁迫与种子大小及其互作对架豆角种子萌发及幼苗早期生长的影响.结果表明:NaCl胁迫显著影响架豆角种子的发芽率、幼苗根长、芽长及鲜重.当NaCl浓度≤50 mmol/L时发芽率的抑制程度不显著,而当NaCl浓度>50 mmol/L时,盐分对发芽率的抑制作用非常明显,NaCl浓度达到150 mmol/L时,种子发芽完全被抑制.幼苗的根长和芽长在所有的NaCl浓度下都表现出显著的抑制,而幼苗的鲜重只是在NaCl浓度>50 mmol/L时才表现出显著的抑制效果.种子大小的差异对架豆角种子幼苗的根长及芽长没有显著的影响,但对发芽率和幼苗的鲜重表现出显著的作用.NaCl胁迫和种子大小的互作在发芽率、幼苗根长、芽长及鲜重4个指标中都没有达到显著水平.

  3. 基于SSR分子标记技术的长豇豆种子纯度快速鉴定技术%A rapid and precise SSR-based procedure for seed quality surveying of Vigna unguiculata ssp.sesquipedalis(L.)Verd.

    Institute of Scientific and Technical Information of China (English)

    鲁忠富; 徐沛; 汪宝根; 刘永华; 吴晓花; 胡婷婷; 李国景

    2010-01-01

    当前生产上应用的长豇豆品种众多,且商业品种间的遗传相似性愈来愈高,种子质量纠纷时有发生.本研究以44份核心长豇豆种质为试材,利用普通豇豆DNA序列信息,通过生物信息学方法自主设计开发适用于长豇豆研究的SSR引物,从中筛选出10对诊断性引物,在此基础上建立基于SSR分子标记技术的长豇豆种子纯度快速榆测方法,可满足长豇豆种子产业化的需要.

  4. 三唑酮浸种对豇豆幼苗苗质和部分生理活性的影响%Effects of Seed-soaking with Triadimefon on the Seedling Quality and Partial Physiological Activities of Vigna unguiculata Seedling

    Institute of Scientific and Technical Information of China (English)

    段辉国; 向彬方; 杜娟; 丁小波; 夏奎; 向利红

    2008-01-01

    [目的] 为进一步探讨三唑酮应用的广泛性及其作用机理提供基础资料.[方法]研究了不同浓度(0、10、20、30、40 mg/L)三唑酮浸种对豇豆幼苗的苗质,叶绿体色素、脯氨酸和可溶性蛋白质含量的影响.[结果] 10、20、30、40 mg/L三唑酮浸种后,豇豆幼苗的株高受到明显抑制,根冠比显著增加,根长有不同程度的降低.除10 mg/L外,经其余浓度三唑酮浸种的豇豆幼苗的叶面积均显著降低,而叶绿素含量均有所提高(以30 mg/L的浸种效果最好).豇豆幼苗的脯氨酸和可溶性蛋白质的含量均随三唑酮浸种浓度的升高而增加,30 mg/L达到最大值.[结论]三唑酮溶液浸种对豇豆幼苗的生长发育有较好的促进作用,其最佳浓度为30 mg/L.

  5. Mapping of quantitative trait loci for phytic acid and phosphorus contents in seed and seedling of mungbean (Vigna radiata (L.) Wilczek)

    OpenAIRE

    Sompong, Utumporn; Somta, Prakit; Raboy, Victor; Srinives, Peerasak

    2012-01-01

    Phytic acid (PA) is the storage form of phosphorus (P) in seeds and plays an important role in the nutritional quality of food crops. There is little information on the genetics of seed and seedling PA in mungbean [Vigna radiata (L.) Wilczek]. Quantitative trait loci (QTL) were identified for phytic acid P (PAP), total P (TP), and inorganic P (IP) in mungbean seeds and seedlings, and for flowering, maturity and seed weight, in an F2 population developed from a cross between low PAP cultivated...

  6. Danos mecânicos em sementes de feijão Vigna, causados pelas operações na unidade de beneficiamento Mechanical damages in Vigna bean seeds caused by the operations in the seed processing

    Directory of Open Access Journals (Sweden)

    Francisco de A. C. Almeida

    2004-12-01

    Full Text Available Depois da colheita e debulha, as sementes de feijão são encaminhadas à usina de beneficiamento, de onde, por elevadores, são conduzidas às máquinas de pré-limpeza, limpeza, separação e classificação, tratamento e embalagem, para posterior armazenamento experimentando durante este percurso, impacto promovidos pela movimentação dos elevadores e das máquinas. Objetivando-se contribuir com o programa de controle de qualidade de sementes de feijão produzidas principalmente no estado da Paraíba, estudaram-se os impactos mecânicos sofrido pelas sementes de duas variedades de feijão Vigna com dois teores de umidade (13,5 e 7,3% depois da colheita, debulha e da passagem pelas diferentes etapas na Empresa de Pesquisa Agropecuária da Paraíba, localizada em Alagoinha, PB. Os danos decorrentes dos impactos foram analisados visualmente, empregando-se um delineamento inteiramente casualizado em esquema fatorial 2 x 2 x 5, submetendo-se as médias dos fatores quantitativos a regressão na análise da variância. As sementes com umidade de 7,3% da variedade Rabo de Tatu são mais susceptíveis à quebra pelos impactos no processo e, às injúrias, elevam-se com o aumento do número de passagem das sementes pelas diferentes etapas à qual estão sujeitas, demonstrando caráter cumulativo, indicando haver relação direta entre o número de choques e seus efeitos.After harvesting and threshing the seeds of beans are taken to a seed processing plant where from by elevator are taken to machines of pre-cleaning, cleaning, separation and classification, treatment and packing for posterior storage. During this course, impacts are promoted by the movement of elevators and machines. With the objective to contribute with quality control program of bean seeds mainly produced in the Paraíba State, the mechanical impacts undergone by seeds of two varieties of Vigna bean with two moisture contents (13.5 and 7.3% were studied after harvesting, threshing

  7. Synergism Among VA Mycorrhiza, Phosphate Solubilizing Bacteria and Rhizobium for Symbiosis with Blackgram (Vigna mungo L.) UnderField Conditions

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A field experiment was conducted at the G.B. Pant University Research Station, Ujhani (U.P.) in rainy (Kharif) season of the year 1994-1995 to study the effect of Rhizobium, VAM (vesicular arbuscular mycorrhiza) and PSB (phosphate solubilizing bacteria) inoculation, with and without P, on blackgram (Vigna mungo L.) seed yield. Phosphorus application in soil with medium P content (5.4 mg kg1) increased nodulation, grain yield, N and P in plant and grain over no phosphorus control. Forty kilograms of P2O5 each hactare recorded an increase of 20.6 % in nodule dry weight, significant increases of 0.35 g kg-1 in N concentration and 1.28 g kg-1 in P concentration of plant over 20 kg P2O5 ha-1. Similar significant increases of 0.59 g kg-1 in grain yield and 0.54 and 0.23 g kg-1 in N and P concentrations of the grain, respectively, over 20 kg P2O5 ha-1 were also obtained with higher dose. Inoculation of Rhizobium + VAM + PSB at all the stages of plant growth recorded maximum increases in all the parameters studied. Dual inoculation of Rhizobium with either VAM or PSB was generally significant in the effect and better than that of VAM + PSB, however, P accumulation in plant and grain was more with VAM + PSB. Among single inoculations, Rhizobium gave highest and 21.0 % more nodule number, 34.7 % more nodule dry mass, 0.73 g kg-1 more N in grain and 4.2 % higher grain yield over PSB. PSB, however, registered significant increases in P concentration in plant and grain over VAM and Rhizobium.

  8. Purification and characterization of a Bowman-Birk proteinase inhibitor from the seeds of black gram (Vigna mungo).

    Science.gov (United States)

    Prasad, E R; Dutta-Gupta, A; Padmasree, K

    2010-03-01

    A proteinase inhibitor (BgPI) was purified from black gram, Vigna mungo (cv. TAU-1) seeds by using ammonium sulfate fractionation, followed by ion-exchange, affinity and gel-filtration chromatography. BgPI showed a single band in SDS-PAGE under non-reducing condition with an apparent molecular mass of approximately 8kDa correlating to the peak 8041.5Da in matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrum. BgPI existed in different isoinhibitor forms with pI values ranging from 4.3 to 6.0. The internal sequence "SIPPQCHCADIR" of a peak 1453.7 m/z, obtained from MALDI-TOF-TOF showed 100% similarity with Bowman-Birk inhibitor (BBI) family. BgPI exhibited non-competitive-type inhibitory activity against both bovine pancreatic trypsin (K(i) of 309.8nM) and chymotrypsin (K(i) of 10.7muM), however, with a molar ratio of 1:2 with trypsin. BgPI was stable up to a temperature of 80 degrees C and active over a wide pH range between 2 and 12. The temperature-induced conformational changes in secondary structure are reversed when BgPI was cooled from 90 to 25 degrees C. Further, upon reduction with dithiothreitol, BgPI lost both its inhibitory activity as well as secondary structural conformation. Lysine residue(s) present in the reactive site of BgPI play an important role in inhibiting the bovine trypsin activity. The present study provides detailed biochemical characteristic features of a BBI type serine proteinase inhibitor isolated from V. mungo.

  9. Induced changes in the antioxidative compounds of Vigna mungo genotypes due to infestation by Bemisia tabaci (Gennadius).

    Science.gov (United States)

    Taggar, Gaurav Kumar; Gill, Ranjit Singh; Gupta, Anil Kumar; Singh, Sarvjeet

    2014-11-01

    Antioxidative compounds were quantified from the leaves of nine black gram (Vigna mungo (L.) Hepper) genotypes over a period of two years, for potential whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleryrodidae) resistance. Oviposition preference, nymphal and adult development were evaluated under screen-house conditions. Biochemical analysis revealed that higher per cent increase in the total phenol and o-dihydroxy phenol contents both at 30 and 50 days after sowing was evident in moderately resistant genotypes NDU 5-7 (49.6 and 50.8%, respectively) and KU 99-20 (47.8 and 50.8%, respectively) under whitefly stress conditions as compared to non-stressed plants. Tannin and flavonol contents in leaves increased to varying degrees (up to 11.1 and 7.1%, respectively) in resistant plants after whitefly infestation, indicating that the changes in tannin and flavonol contents were closely associated with the resistance to whitefly. Correlation studies relating leaf content of black gram antioxidative compounds from different genotypes with whitefly population were also worked out. Total phenols (r = -0.71 & -0.88), o- dihydroxy phenols (r = -0.56 & -0.76), flavonols (r = -0.80 & -0.81) and tannins (r= -0.16 & -0.26) showed significant negative correlation with whitefly population (nymphs and adults) suggesting that enhanced level of these biochemicals may contribute to bioprotection of black gram plants against B. tabaci infestation. Comparatively higher level of resistance in genotype NDU 5-7 and KU 99-20 can serve as base for genetic improvement of black gram, focusing on the development of resistant varieties to B. tabaci.

  10. The role of VuMATE1 expression in aluminium-inducible citrate secretion in rice bean (Vigna umbellata) roots.

    Science.gov (United States)

    Liu, Mei Ya; Chen, Wei Wei; Xu, Jia Meng; Fan, Wei; Yang, Jian Li; Zheng, Shao Jian

    2013-04-01

    Aluminium (Al)-activated citrate secretion plays an important role in Al resistance in a number of plant species, such as rice bean (Vigna umbellata). This study further characterized the regulation of VuMATE1, an aluminium-activated citrate transporter. Al stress induced VuMATE1 expression, followed by the secretion of citrate. Citrate secretion was specific to Al stress, whereas VuMATE1 expression was not, which could be explained by a combined regulation of VuMATE1 expression and Al-specific activation of VuMATE1 protein. Pre-treatment with a protein translation inhibitor suppressed VuMATE1 expression, indicating that de novo biosynthesis of proteins is required for gene expression. Furthermore, post-treatment with a protein translation inhibitor inhibited citrate secretion, indicating that post-transcriptional regulation of VuMATE1 is critical for citrate secretion. Protein kinase and phosphatase inhibitor studies showed that reversible phosphorylation was important not only for transcriptional regulation of VuMATE1 expression but also for post-translational regulation of VuMATE1 protein activity. These results suggest that citrate secretion is dependent on both transcriptional and post-transcriptional regulation of VuMATE1. Additionally, VuMATE1 promoter-β-glucuronidase fusion lines revealed that VuMATE1 expression was restricted to the root apex and was entirely Al induced, indicating the presence of cis-acting elements regulating root tip-specific and Al-inducible gene expression, which will be an important resource for genetic improvement of plant Al resistance.

  11. Genotypic difference in (137)Cs accumulation and transfer from the contaminated field in Fukushima to azuki bean (Vigna angularis).

    Science.gov (United States)

    Win, Khin Thuzar; Oo, Aung Zaw; Kojima, Katsuhiro; Salem, Djedidi; Yamaya, Hiroko; Bellingrath-Kimura, Sonoko Dorothea; Tomooka, Norihiko; Kaga, Akito; Ohkama-Ohtsu, Naoko; Yokoyama, Tadashi

    2016-07-01

    The screening of mini-core collection of azuki bean accessions (Vigna angularis (Willd.) Ohwi & Ohashi) for comparative uptake of (137)Cs in their edible portions was done in field trials on land contaminated by the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident. Ninety seven azuki bean accessions including their wild relatives from a Japanese gene bank, were grown in a field in the Fukushima prefecture, which is located approximately 51 km north of FDNPP. The contamination level of the soil was 3665 ± 480 Bq kg(-1) dry weight ((137)Cs, average ± SD). The soil type comprised clay loam, where the sand: silt: clay proportion was 42:21:37. There was a significant varietal difference in the biomass production, radiocaesium accumulation and transfer factor (TF) of radiocaesium from the soil to edible portion. Under identical agricultural practice, the extent of (137)Cs accumulation by seeds differed between the accessions by as much as 10-fold. Inter-varietal variation was expressed at the ratio of the maximum to minimum observed (137)Cs transfer factor for seeds ranged from 0.092 to 0.009. The total biomass, time to flowering and maturity, and seed yield had negative relationship to (137)Cs activity concentration in seeds. The results suggest that certain variety/varieties of azuki bean which accumulated less (137)Cs in edible portion with preferable agronomic traits are suitable to reduce the (137)Cs accumulation in food chain on contaminated land. PMID:27105146

  12. Revisión taxonómica de los géneros Phaseolus y Vigna (Leguminosae-Papilionoideae en Cuba

    Directory of Open Access Journals (Sweden)

    Beyra, Ángela

    2004-12-01

    Full Text Available We present a taxonomic study of the Cuban species of Phaseolus L. and Vigna Savi. It includes keys, descriptions, illustrations of selected taxa, chromosome numbers, palinology, phenology, ecology, distribution maps, notes on chemical composition and ethnobotany for each of the eleven species known from Cuba. We also present a discussion on the value of the characters used in the taxonomy of the complex.Se hace una revisión taxonómica de las especies de los géneros Phaseolus L. y Vigna Savi presentes en Cuba. Se incluyen claves dicotómicas, descripciones, ilustraciones, datos químicos, cromosomáticos, palinológicos, etnobotánicos, así como la distribución y ecología de las 11 especies de ambos géneros. Se analiza y se hace una estimación del valor de los caracteres que diferencian a cada uno de los géneros y especies.

  13. Leaf crinkle disease in urdbean (Vigna mungo L. Hepper): An overview on causal agent, vector and host.

    Science.gov (United States)

    Gautam, Narinder Kumar; Kumar, Krishna; Prasad, Manoj

    2016-05-01

    Urdbean leaf crinkle disease (ULCD) is an economically significant widespread and devastating disease resulting in extreme crinkling, puckering and rugosity of leaves inflicting heavy yield losses annually in major urdbean-producing countries of the world. This disease is caused by urdbean leaf crinkle virus (ULCV). Urdbean (Vigna mungo L. Hepper) is relatively more susceptible than other pulses to leaf crinkle disease. Urdbean is an important and useful crop cultivated in various parts of South-East Asia and well adapted for cultivation under semi-arid and subtropical conditions. Aphids, insects and whiteflies have been reported as vectors of the disease. The virus is also transmitted through sap inoculation, grafting and seed. The loss in seed yield in ULCD-affected urdbean crop ranges from 35 to 81%, which is dependent upon type of genotype location and infection time. The diseased material and favourable climatic conditions contribute for the widespread viral disease. Anatomical and biochemical changes take place in the affected diseased plants. Genetic variations have been reported in the germplasm screening which suggest continuous screening of available varieties and new germplasm to search for new traits (new genes) and identify new sources of disease resistance. There are very few reports on breeding programmes for the development and release of varieties tolerant to ULCD. Mostly random amplified polymorphic DNA (RAPD) as well as inter-simple sequence repeat (ISSR) molecular markers have been utilized for fingerprinting of blackgram, and a few reports are there on sequence-tagged micro-satellite site (STMS) markers. There are so many RNA viruses which have also developed strategies to counteract silencing process by encoding suppressor proteins that create hindrances in the process. But, in the case of ULCV, there is no report available indicating which defence pathway is operating for its resistance in the plants and whether same silencing suppression

  14. Leaf crinkle disease in urdbean (Vigna mungo L. Hepper): An overview on causal agent, vector and host.

    Science.gov (United States)

    Gautam, Narinder Kumar; Kumar, Krishna; Prasad, Manoj

    2016-05-01

    Urdbean leaf crinkle disease (ULCD) is an economically significant widespread and devastating disease resulting in extreme crinkling, puckering and rugosity of leaves inflicting heavy yield losses annually in major urdbean-producing countries of the world. This disease is caused by urdbean leaf crinkle virus (ULCV). Urdbean (Vigna mungo L. Hepper) is relatively more susceptible than other pulses to leaf crinkle disease. Urdbean is an important and useful crop cultivated in various parts of South-East Asia and well adapted for cultivation under semi-arid and subtropical conditions. Aphids, insects and whiteflies have been reported as vectors of the disease. The virus is also transmitted through sap inoculation, grafting and seed. The loss in seed yield in ULCD-affected urdbean crop ranges from 35 to 81%, which is dependent upon type of genotype location and infection time. The diseased material and favourable climatic conditions contribute for the widespread viral disease. Anatomical and biochemical changes take place in the affected diseased plants. Genetic variations have been reported in the germplasm screening which suggest continuous screening of available varieties and new germplasm to search for new traits (new genes) and identify new sources of disease resistance. There are very few reports on breeding programmes for the development and release of varieties tolerant to ULCD. Mostly random amplified polymorphic DNA (RAPD) as well as inter-simple sequence repeat (ISSR) molecular markers have been utilized for fingerprinting of blackgram, and a few reports are there on sequence-tagged micro-satellite site (STMS) markers. There are so many RNA viruses which have also developed strategies to counteract silencing process by encoding suppressor proteins that create hindrances in the process. But, in the case of ULCV, there is no report available indicating which defence pathway is operating for its resistance in the plants and whether same silencing suppression

  15. Chinos y gamines: imágenes de los habitantes pobres de Bogotá en la primera mitad del siglo XX The urchins and street boys: images of the poor inhabitants of Bogota in the first half of the 20th century

    Directory of Open Access Journals (Sweden)

    Yeimy Cárdenas Palermo

    2012-04-01

    Full Text Available En el artículo se tejen algunos indicios que permiten poner en escena las formas como los niños pobres o los hijos de los habitantes pobres de la ciudad de Bogotá fueron diferenciados de la infancia "normal", en la primera mitad del siglo XX. Acudiendo a discursos de especialistas, voces de reformistas educativos y periodistas se evidencia que la diferenciación fue producida mediante la estigmatización de los lugares sociales de los niños pobres, a través de la imagen de los chinos y los gamines. Imágenes que resultan paradigmáticas para desentrañar la deslegitimación de las estructuras familiares, de las trayectorias escolares y de los modos de habitar la ciudad de los niños y niñas de los sectores populares, dada la articulación, infundada y temeraria que se estableció entre la vida de los pobres y la delincuencia infantil.In this paper we present hints that allow us to reveal the ways poor children, or the children of poor inhabitants, from the city of Bogota were excluded from the "normal" childhood in the first half of the 20th century. Based on specialists' statements as well as opinions from educational reformers and journalists it is clear that this exclusion was created due to the stigmatization of the social places belonging to the poor children through the image of the urchins and street boys.. Such images were a model that could reveal the illegitimacy of the family structures, of the school history and of the way children from the popular areas lived, given the articulation, mistaken and dangerous, that was established between the life of poor people and children delinquency.

  16. Efficacy of Carbofuran in Controlling Root-Knot Nematode (Meloidogyne javanica Whitehead, 1949 on Cultivars of Bambara Groundnut (Vigna subterranea (L. Verdc. in Yola, Nigeria

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    M. Y. Jada

    2011-01-01

    Full Text Available Bambara groundnut (Vigna subterrenea L. Verdc. is an important crop produced in Adamawa State of Nigeria. However, the production of the crop is seriously threatened by root-knot nematodes (RKNs; Meloidogyne spp.. Since cultural methods have not been very effective in controlling RKN, carbofuran was evaluated to determine its efficacy in controlling M. javanica in Yola during 2002 and 2003. Three bambara groundnut cultivars (Kwachanjiwa, Kwaheuma, and Kwatolotolo were evaluated using three application timings (at planting, 3 and 6 weeks after planting, and none. Results indicated that applying carbofuran at planting provided the greatest reduction in M. javanica population levels, which lead to increased yields in bambara groundnuts compared to the other two application timings. Furthermore, both Kwachanjiwa and Kwatolotolo provided similar high yields compared to Kwaheuma, which was most likely related to the M. javanica tolerance in these cultivars.

  17. Studies on the management of root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex of green gram, Vigna radiata cv ML-1108

    Institute of Scientific and Technical Information of China (English)

    HASEEB Akhtar; SHARMA Anita; SHUKLA Prabhat Kumar

    2005-01-01

    Studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil,bavistin at 1 mg a.i./kg soil, neem (Azadirachta indica) seed powder at 50 mg/kg soil, green mould (Trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (Pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex on green gram, Vigna radiata cv ML-1108. All the treatments significantly improved the growth of the plants as compared to untreated inoculated plants. Analysis of data showed that carbofuran and A. indica seed powder increased plant growth and yield significantly more in comparison to bavistin and P.fluorescens. Carbofuran was highly effective against nematode, bavistin against fungus, A. indica seed powder against both the pathogens and both the bioagents were moderately effective against both the pathogens.

  18. Assessment of peroxidase isozyme marker-based model for cross identifications in hybrids (F(1)) of urdbean [ Vigna mungo (L.) Hepper].

    Science.gov (United States)

    Upadhyay, R.; Shukla, A.; Gaur, K.

    2002-12-01

    Four hybrids (4 F(1)s) were chosen out of crosses in the urdbean [ Vigna mungo (L.) Hepper, 2n = 22] having contrasting morphological characters. Zymograms for isozyme peroxidase were drawn from the patterns obtained from parents and their respective F(1) hybrids on the basis of relative similarities to parental bands. The selfed or crossed nature of hybrid pods was determined from the zymograms and their analysis. The number of bands and their intensities gave an idea about the extent of crossing in F(1) populations. Genetic identity (I) values were indicative of their selfed nature. Dendrograms were constructed on the basis of genetic identity values to display the relative similarities between the populations. Analysis was based on individual pods to confirm their hybrid or selfed nature. Possible use of this technique for identification of F(1) pods and elimination of selfed pods might be implemented to shorten the breeding operations during crossing.

  19. Isolation and screening of rhizobia for auxin biosynthesis and growth promotion of mung bean (Vigna radiata L. seedlings under axenic conditions

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    Muhammad Ashfaq Anjum, Zahir Ahmad Zahir, Muhammad Arshad and Muhammad Ashraf

    2011-04-01

    Full Text Available A series of screening experiments to evaluate the effectiveness of rhizobia for producing auxins and improvegrowth and nodulation of mungbean (Vigna radiata L. were carried out under axenic conditions. Forty fouriolatess of rhizobia were isolated using standard procedures. Auxin biosynthesis by these rhizobial isolates wasdetermined in the absence and presence of L-Trp, a physiological precursor of auxins. Rhizobial isolates variedwidely in auxins biosynthesis capabilities. On the basis of auxins biosynthesis, a pouch experiment was conductedfor screening thirty four efficient isolates of rhizobia for the growth promotion of mung bean. Results of pouch studyshowed that inoculation with selected rhizobial isolates increased the root /shoot length, fresh, and dry shoot weightof mung bean up to 33, 59, 71, 148, 107 and 188%, respectively, over untreated control. Further studies are neededunder glasshouse and field conditions for confirmation of these results.

  20. Observations préliminaires de la variabilité entre quelques morphotypes de voandzou (Vigna subterranea L. Verdc., Fabaceae de Côte d'Ivoire

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    Zoro Bi IA.

    2005-01-01

    Full Text Available Preliminary observations of variability between some morphotypes of bambara groundnut (Vigna subterranea L. Verdc., Fabaceae from Côte d’Ivoire. Bambara groundnut (Vigna subterranea L. Verdc., is a food legume mainly cultivated by women for whom it represents a source of income for the household. In Côte d’Ivoire, the cultivation of bambara groundnut is located in the western and northern parts of the country. These zones are characterised by contrasted agroecology including tropical rain forest and dry savanna. In these zones, bambara groundnut plays a key role in both food and culture of peoples. Four morphotypes of Côte d’Ivoire (ICU, BPR, RBU, NFU were used in a preliminary study to assess the phenotypic variability between morphotypes. For each morphotype, 100 individuals were sampled to analyse 26 agromorphological traits selected from the list of bambara groundnut descriptors. Results of statistical analyses showed an important variability among morphotypes suggesting that 22 of these characters could be powerful to distinguish diversity among bambara groundnut morphotypes of Côte d’Ivoire. Three morphotypes (ICU, BPR and RBU show a shorter reproductive cycle than the other (NFU. In our experimental conditions, morphotypes with a shorter reproductive cycle give a higher percentage of matured pods (87 to 95%, compared to morphotype NFU (60%. The morphotype ICU was particularly earlier, maturing 90 days after sowing (DAS, whereas the long reproductive cycle morphotype (NFU required about 137 days. Based on the analysed agronomic traits, possibilities to improve bambara groundnut yield and to promote its cultivation in Côte d’Ivoire are discussed.

  1. Evaluation of hepatoprotective and nephroprotective activity of aqueous extract of Vigna mungo (Linn. Hepper on rifampicin-induced toxicity in albino rats

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    M Nitin

    2012-01-01

    Full Text Available Aim: The objective of this study was to investigate the hepatoprotective and nephroprotective activity of aqueous extract of seeds of Vigna mungo (AEVM (fabaceae against rifampicin-induced liver and kidney damage in rats. Settings and Design: Albino rats of either sex (200-250 g were selected and divided in to eight groups of six animals in each. Four groups for hepatoprotective activity and four groups for nephroprotective activity. Group 1 was normal control, group 2 was positive control, group 3 was treated with standard drug, group 4 was treated with AEVM. Similarly it was done for nephroprotective activity. The results are evidenced on the basis of physical, biochemical, histological, and functional parameters. Materials and Methods: Drugs used are rifampicin, silymarin, diagnostic kits (SGPT, SGOT, ALP, and BIT for hepatoprotective activity. BUN, serum creatinine, and serum uric acid for nephroprotective activity. Seed powder of Vigna mungo was extracted with water. Preliminary phytochemical tests were done to identify the phytoconstituents. The hepatoprotective and nephroprotective activity of the AEVM were assessed in rifampicin-induced hepatotoxic and nephrotoxic rats. Statistical Analysis Used: One-way analysis of variance (ANOVA followed by "Tukey-Kramer" multiple comparison tests. Results: The AEVM showed the presence of amino acids, alkaloids, carbohydrates, flavonoids, glycosides, proteins, phytic acid, total phenolic compounds, saponins, and tannins. Rifampicin produced significant changes in physical (increased liver weight, decreased body weight, biochemical (increase in serum glutathione pyruvate transaminase (SGPT, oxaloacetate transaminase (SGOT, alkaline phosphatase (ALP and total bilirubin (BIT level, increase in blood urea nitrogen (BUN, serum creatinine, and serum uric acid level, histological (damage to hepatocytes, nephrons, and functional (barbiturates-induce sleeping time induced by rifampicin in liver and kidney

  2. Aspectos epidemiológicos das micoses dos pés em um time chinês de futebol Aspectos epidemiologicos de las micosis de los pies en un equipo chino de fútbol Epidemiological aspects of the feet mycosis in a Chinese soccer team

    Directory of Open Access Journals (Sweden)

    Kátia Sheylla Malta Purim

    2006-02-01

    descriptivo. La muestra estaba compuesta de 22 hombres, con las edades entre 23 y 36 años, íntegrantes del equipo de fútbol profesional chino, cuando participaron en juegos en Curitiba (PR. Todos fueron evaluados clínicamente y sometidos a los exámenes micológicos respectivos (directo y cultivo de escamas de piel y uña de los pies y estudio histopatológico de fragmento ungueal. Los resultados que se encontraron fueron: 12 casos (54,5% que no presentaron micosis; 5 casos (22,72% que presentaron onicomicosis aislada y 5 casos (22,72% que presentaron onicomicosis asociada a tinea pedis, que tiene como agente aislado principal el Trichophyton rubrum. Los factores predisponentes para los atletas incluidos consignados fueron: bañarse en lugares públicos (85%, la práctica deportiva (76%, el uso de zapatos cerrados (70%, tener un animal doméstico (63% y la irregularidad en la higiene de los pies (50%. CONCLUSIONES: La frecuencia de la onicomicosis en los atletas chinos es más grande que en la población en general. El agente más frecuente fué el Trichophyton rubrum. Los hábitos individuales pueden contribuir a la adquisición de esas infecciones fúngicas, además del trauma directo por el zapato, así como el contacto físico durante los entrenamientos y juegos que pueden favorecer lesiones cutáneas y ungueales.Podal fungal infections can interfere in the sportive performance, since they provoke discomfort and pain in the athletes. The purpose of this study was to assess the prevalence of podal fungal infections in a professional soccer team, verifying the more frequent agents and the predisposing factors to that condition. The research had a transversal and descriptive outlining. The sampling was composed by twenty-two 23 to 36 years old male athletes from the professional Chinese soccer team on the occasion they participated in a game series in Curitiba (PR. All athletes were clinically assessed, and they were submitted to mycological examinations (direct and

  3. Biogenic green synthesis of monodispersed gum kondagogu (Cochlospermum gossypium) iron nanocomposite material and its application in germination and growth of mung bean (Vigna radiata) as a plant model.

    Science.gov (United States)

    Raju, Dugyala; Mehta, Urmil J; Beedu, Sashidhar Rao

    2016-06-01

    An eco-friendly green and one-pot synthesis of highly monodispersed iron (Fe) nanoparticles (NPs) by using a natural biopolymer, gum kondagogu (GK) as reducing and capping agent is proposed. The NPs synthesised were characterised by ultra-violet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy and X-ray diffraction. As the concentration of gum and time increases, the intensity of NPs formation increased. The NPs were highly monodispersed with uniform circular shapes of 2-6 nm in size. The formed NPs were crystalline in nature which was confirmed by diffraction analysis. The conversion ratio of Fe ionic form to NPs was 21% which was quantified by inductively coupled plasma mass spectroscopy (ICP-MS). Fe is essential for plant growth and development. A study was conducted to examine the effect of these NPs on the growth of mung bean (Vigna radiata). The radical length and biomass was increased in seeds exposed to Fe NPs than the ions. The uptake of Fe NPs by the sprouts was also quantified by ICP-MS, in which Fe was more in mung bean seeds exposed to NPs. The α-amylase activity was increased in the seeds exposed to NPs. The observed increase in the biomass by Fe NPs and seed germination may facilitate its application in the agriculture as an important cost-effective method for plant growth. PMID:27256894

  4. Polyamines Confer Salt Tolerance in Mung Bean (Vigna radiata L.) by Reducing Sodium Uptake, Improving Nutrient Homeostasis, Antioxidant Defense, and Methylglyoxal Detoxification Systems

    Science.gov (United States)

    Nahar, Kamrun; Hasanuzzaman, Mirza; Rahman, Anisur; Alam, Md. Mahabub; Mahmud, Jubayer-Al; Suzuki, Toshisada; Fujita, Masayuki

    2016-01-01

    The physiological roles of PAs (putrescine, spermidine, and spermine) were investigated for their ability to confer salt tolerance (200 mM NaCl, 48 h) in mung bean seedlings (Vigna radiata L. cv. BARI Mung-2). Salt stress resulted in Na toxicity, decreased K, Ca, Mg, and Zn contents in roots and shoots, and disrupted antioxidant defense system which caused oxidative damage as indicated by increased lipid peroxidation, H2O2 content, O2•- generation rate, and lipoxygenase activity. Salinity-induced methylglyoxal (MG) toxicity was also clearly evident. Salinity decreased leaf chlorophyll (chl) and relative water content (RWC). Supplementation of salt affected seedlings with exogenous PAs enhanced the contents of glutathione and ascorbate, increased activities of antioxidant enzymes (dehydroascorbate reductase, glutathione reductase, catalase, and glutathione peroxidase) and glyoxalase enzyme (glyoxalase II), which reduced salt-induced oxidative stress and MG toxicity, respectively. Exogenous PAs reduced cellular Na content and maintained nutrient homeostasis and modulated endogenous PAs levels in salt affected mung bean seedlings. The overall salt tolerance was reflected through improved tissue water and chl content, and better seedling growth.

  5. Nutritive Evaluation of the Bambara Groundnut Ci12 Landrace [Vigna subterranea (L. Verdc. (Fabaceae] Produced in Côte d’Ivoire

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    Denis N'Dri Yao

    2015-09-01

    Full Text Available The nutritional evaluation of the Bambara groundnut Ci12 landrace (Vigna subterranea (L. Verdc. seeds produced in Côte d’Ivoire shows a 19% content of protein, containing all the essential amino acids with tryptophan as the limiting amino acid, a total dietary fiber level of 10%, with a low soluble fraction content, and a fat content of 1.4%, with a high proportion of total unsaturated fatty acids (61% of which 36% were n-6 fatty acids. This legume contains phosphorus, as the major mineral, followed by magnesium and calcium, and trace elements (iron, copper and zinc. It is characterized by the same amount of α-tocopherol and antioxidant capacity as common legumes. The high concentration of essential amino acids, n-6 fatty acids and minerals, mainly Fe, in the Ci12 landrace of Bambara groundnut indicates that this local legume has the potentiality to improve the nutritional status in Côte d’Ivoire and it could be regarded as a nutrient dense food.

  6. Impact of pesticides on plant growth promotion of Vigna radiata and non-target microbes: comparison between chemical- and bio-pesticides.

    Science.gov (United States)

    Gupta, Sukriti; Gupta, Rashi; Sharma, Shilpi

    2014-08-01

    To compare the target and non-target effects of two chemical-pesticides (chlorpyrifos and endosulfan) with that of a bio-pesticide (azadirachtin), Vigna radiata (mung bean) was grown in a randomized pot experiment with recommended and higher application rates of pesticides. Colony counts enumerating specific microbial populations, viz. fungi, Pseudomonas, nitrogen-fixing bacteria, and phosphate-solubilizing microorganisms, were performed. In addition, several plant growth parameters such as root and shoot lengths were also monitored. It was observed that the pesticides exerted a suppressive effect on different microbial communities under study in the initial 30 days period. The bacterial and fungal populations in chlorpyrifos treated plants increased thereafter. Endosulfan resulted in enhancement of fungi and nitrogen-fixing bacteria, although phosphate-solubilizing microorganisms were suppressed at higher application rates. Azadirachtin, which is gaining popularity owing to its biological origin, did not result in enhancement of any microbial populations; on the other hand, it had a deleterious effect on phosphate-solubilizing bacteria. This study is the first to evaluate the non-target effects of pesticides with a comparison between chemical- and bio-pesticides, and also stresses the importance of critical investigation of bio-pesticides before their wide spread application in agriculture. PMID:24799184

  7. Effects of Processing (Boiling and Roasting on the Nutritional and Antinutritional Properties of Bambara Groundnuts (Vigna subterranea [L.] Verdc. from Southern Kaduna, Nigeria

    Directory of Open Access Journals (Sweden)

    Uche Samuel Ndidi

    2014-01-01

    Full Text Available This research analyzed the effect of processing (boiling and roasting on the proximate, antinutrient, and mineral composition of Vigna subterranea seeds. The proximate composition analysis showed significant difference (P<0.05 between the levels of crude lipid, crude fiber, gross energy, carbohydrate, and moisture content in the raw and processed V. subterranea. However, no significant difference (P<0.05 was observed in protein content of processed V. subterranea as compared to the raw seeds. Analyses of antinutrient composition show that processing significantly reduced the levels of oxalate, tannins, phytate, trypsin inhibitor, and hydrogen cyanide contents of V. subterranea. While magnesium, potassium, and phosphorus were the most abundant macrominerals in V. subterranea, zinc was the most abundant micromineral. Correlation analysis revealed that the levels of crude protein, ash, moisture, and lipid were negatively affected by phytate, tannins, and oxalate. The results, therefore, suggest that processing significantly lowered the levels of antinutrients in V. subterranea, thereby making it safer for consumption.

  8. The Aquaeous Extract of Root Nodules Vigna radiata (rnVr which Inoculated by Rhizobium as an Orally Available Anemia Therapeutic Candidate

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    Dewi Hidayati

    2015-11-01

    Full Text Available The extract of root nodules Vigna radiata (rnVr which inoculated by Rhizobium is considered beneficial as an orally available anemia therapeutic candidate, because it contain the leghemoglobin. The positive control mice (group I were fed with the high nutrient pellet.The twelve mice (Mus musculus was treated with the “taking rice pellet” that representing the low nutrient food for 21 days until they suffered anemia. Then, the anemia mice were treated orally with rnVr in different concentration groups:II. 0% III.33%; IV.67% and V.100%, respectively and fed with the “aking rice pellet”. After 14 days, the blood mice were collected from orbital sinus. The hemoglobin (Hb concentration were analyzed by spectrophotometry and blood plasma profile protein were analyzed with electrophoresis (SDS-PAGE. All anemia mice that treated with rnVr showed the increasing of Hb and group that treated with 100% extract of rnVr could reach a normal Hb value, raising from 9.85 to 12.68 g/dL. There were observed the proteins which have molecule weight 36.5 and 35.7 kDa that indicated the existing erythropoietin. The increasing haemoglobin concentration and erythropoietin suggested if extract of rnVr could increasing red blood production and potential as an orally available anemia therapeutic candidate.

  9. Cotyledon cells of Vigna mungo seedlings use at least two distinct autophagic machineries for degradation of starch granules and cellular components.

    Science.gov (United States)

    Toyooka, K; Okamoto, T; Minamikawa, T

    2001-09-01

    alpha-Amylase is expressed in cotyledons of germinated Vigna mungo seeds and is responsible for the degradation of starch that is stored in the starch granule (SG). Immunocytochemical analysis of the cotyledon cells with anti-alpha-amylase antibody showed that alpha-amylase is transported to protein storage vacuole (PSV) and lytic vacuole (LV), which is converted from PSV by hydrolysis of storage proteins. To observe the insertion/degradation processes of SG into/in the inside of vacuoles, ultrastructural analyses of the cotyledon cells were conducted. The results revealed that SG is inserted into LV through autophagic function of LV and subsequently degraded by vacuolar alpha-amylase. The autophagy for SG was structurally similar to micropexophagy detected in yeast cells. In addition to the autophagic process for SG, autophagosome-mediated autophagy for cytoplasm and mitochondria was detected in the cotyledon cells. When the embryo axes were removed from seeds and the detached cotyledons were incubated, the autophagosome-mediated autophagy was observed, but the autophagic process for the degradation of SG was not detected, suggesting that these two autophagic processes were mediated by different cellular mechanisms. The two distinct autophagic processes were thought to be involved in the breakdown of SG and cell components in the cells of germinated cotyledon.

  10. Isolation, characterization, and structure analysis of a non-TIR-NBS-LRR encoding candidate gene from MYMIV-resistant Vigna mungo.

    Science.gov (United States)

    Maiti, Soumitra; Paul, Sujay; Pal, Amita

    2012-11-01

    Yellow mosaic disease of Vigna mungo caused by Mungbean yellow mosaic India virus (MYMIV) is still a major threat in the crop production. A candidate disease resistance (R) gene, CYR1 that co-segregates with MYMIV-resistant populations of V. mungo has been isolated. CYR1 coded in silico translated protein sequence comprised of 1,176 amino acids with coiled coil structure at the N-terminus, central nucleotide binding site (NBS) and C-terminal leucine-rich repeats (LRR) that belongs to non-TIR-NBS-LRR subfamily of plant R genes. CYR1 transcript was unambiguously expressed during incompatible plant virus interactions. A putative promoter-like sequence present upstream of this candidate gene perhaps regulates its expression. Enhanced transcript level upon MYMIV infection suggests involvement of this candidate gene in conferring resistance against the virus. In silico constructed 3D models of NBS and LRR regions of this candidate protein and MYMIV-coat protein (CP) revealed that CYR1-LRR forms an active pocket and successively interacts with MYMIV-CP during docking, like that of receptor-ligand interaction; indicating a critical role of CYR1 as signalling molecule to protect V. mungo plants from MYMIV. This suggests involvement of CYR1 in recognizing MYMIV-effector molecule thus contributing to incompatible interaction. This study is the first stride to understand molecular mechanism of MYMIV resistance.

  11. Effect of formulated root endophytic fungus Piriformospora indica and plant growth promoting rhizobacteria fluorescent pseudomonads R62 and R81 on Vigna mungo.

    Science.gov (United States)

    Kumar, Vinod; Sarma, M V R K; Saharan, Krishna; Srivastava, Rashmi; Kumar, Lalit; Sahai, Vikram; Bisaria, V S; Sharma, A K

    2012-02-01

    In the present investigation, the effect of three beneficial organisms (root endophytic fungus Piriformospora indica (Pi) and pseudomonads strains R62 and R81) and their four different consortia (Pi+R62, Pi+R81, R62+R81, Pi+R62+R81) was investigated on the plant Vigna mungo through their inorganic carrier-based (talcum powder and vermiculite) formulations. All the treatments resulted in significant increase in growth parameters under glasshouse as well as field conditions and showed a consistency in their performance on moving from glasshouse to field conditions. In glasshouse conditions, a maximum increase of 4.5-fold in dry root weight and 3.9-fold in dry shoot weight compared to control was obtained with vermiculite-based consortium formulation of Pi+R81. In field studies using vermiculite as carrier, a maximum enhancement of 3.2-fold in dry root weight, 3.0-fold in dry shoot weight, 8.4-fold in number of nodules and 4.0-fold in number of pods in comparison to control was obtained with the bio-inoculant formulation containing consortium of Pi+R81. The same treatment also caused the highest improvement of 1.9-fold in nitrogen content and 1.7-fold in phosphorus content, while the highest increase of 1.4-fold in potassium content was obtained with Pi alone.

  12. Fluctuations in peroxidase and catalase activities of resistant and susceptible black gram (Vigna mungo (L.) Hepper) genotypes elicited by Bemisia tabaci (Gennadius) feeding.

    Science.gov (United States)

    Taggar, Gaurav Kumar; Gill, Ranjit Singh; Gupta, Anil Kumar; Sandhu, Jeet Singh

    2012-10-01

    Whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleryrodidae), is a serious pest of black gram, (Vigna mungo (L.) Hepper), an important legume pulse crop grown in north India. This research investigated the potential role of selected plant oxidative enzymes in resistance/susceptibility to whitefly in nine black gram genotypes. Oxidative enzyme activity was estimated spectrophotometrically from leaf samples collected at 30 and 50 d after sowing (DAS) from whitefly infested and uninfested plants. The enzymes showed different activity levels at different times after the infestation. The results indicated that in general, whitefly infestation increased the activities of peroxidase and decreased the catalase activity. Resistant genotypes NDU 5-7 and KU 99-20 recorded higher peroxidase and catalase activities at 30 and 50 DAS under whitefly-stress conditions as compared with non-stressed plants. The results suggest that the enhanced activities of the enzymes may contribute to bioprotection of black gram plants against B. tabaci infestation. The potential mechanisms to explain the correlation of resistance to whitefly in black gram genotypes with higher activities of oxidative enzymes are also discussed.

  13. Transformation of a recalcitrant grain legume, Vigna mungo L. Hepper, using Agrobacterium tumefaciens-mediated gene transfer to shoot apical meristem cultures.

    Science.gov (United States)

    Saini, Raman; Jaiwal, Pawan K

    2005-06-01

    The efficiency of Vigna mungo L. Hepper transformation was significantly increased from an average of 1% to 6.5% by using shoot apices excised from embryonic axes precultured on 10 microM benzyl-6-aminopurine (BAP) for 3 days and wounded prior to inoculation in Agrobacterium tumefaciens strain EHA105 carrying the binary vector pCAMBIA2301, which contains a neomycin phosphotransferase gene (nptII) and a beta-glucuronidase (GUS) gene (gusA) interrupted by an intron. The transformed green shoots that were selected and rooted on medium containing kanamycin, and which tested positive for nptII gene by polymerase chain reaction, were established in soil to collect seeds. GUS activity was detected in whole T(0) shoots and T(1) seedlings. All T(0) plants were morphologically normal, fertile and the majority of them transmitted transgenes in a 3:1 ratio to their progenies. Southern analysis of T(1) plants showed integration of nptII into the plant genome.

  14. Transformation of blackgram (Vigna mungo (L.) Hepper) by barley chitinase and ribosome-inactivating protein genes towards improving resistance to Corynespora leaf spot fungal disease.

    Science.gov (United States)

    Chopra, Rajan; Saini, Raman

    2014-12-01

    Blackgram (Vigna mungo (L.) Hepper), an important grain legume crop, is sensitive to many fungal pathogens including Corynespora cassiicola, the causal agent of corynespora leaf spot disease. In the present study, plasmid pGJ42 harboring neomycin phosphotransferase (nptII) a selectable marker gene, the barley antifungal genes chitinase (AAA56786) and ribosome-inactivating protein (RIP; AAA32951) were used for the transformation, to develop fungal resistance for the first time in blackgram. The presence and integration of transgene into the blackgram genome was confirmed by PCR and Southern analysis with an overall transformation frequency of 10.2 %. Kanamycin selection and PCR analysis of T0 progeny revealed the inheritance of transgene in Mendelian fashion (3:1). Transgenic plants (T1), evaluated for fungal resistance by in vitro antifungal assay, arrested the growth of C. cassiicola up to 25-40 % over the wild-type plants. In fungal bio-assay screening, the transgenic plants (T1) sprayed with C. cassiicola spores showed a delay in onset of disease along with their lesser extent in terms of average number of diseased leaves and reduced number and size of lesions. The percent disease protection among different transformed lines varies in the range of 27-47 % compare to control (untransformed) plants. These results demonstrate potentiality of chitinase and RIP from a heterologous source in developing fungal disease protection in blackgram and can be helpful in increasing the production of blackgram.

  15. Isolation and characterization of a cDNA clone of UDP-galactose: flavonoid 3-O-galactosyltransferase (UF3GaT) expressed in Vigna mungo seedlings.

    Science.gov (United States)

    Mato, M; Ozeki, Y; Itoh, Y; Higeta, D; Yoshitama, K; Teramoto, S; Aida, R; Ishikura, N; Shibata, M

    1998-11-01

    Four cDNA clones were isolated from Vigna mungo seedlings by the screening with cDNA encoding UDP-glucose:flavonoid 3-O-glucosyltransferase (UF3GT) of Antirrhinum majus as a probe; the product of the gene corresponding to one cDNA was more highly expressed in the first simple leaves than in stems. Nucleotide sequence analysis revealed 1,691 bp (including 326 bp non-reading) containing an open reading frame of 455 amino acids. The deduced amino acid sequence showed 42% and 23% identity with those of A. majus UDP-glucose:flavonoid 3-O-glucosyltransferase (UF3GT) and Petunia hybrida UDP-rhamnose:anthocyanidin 3-O-glucoside rhamnosyltransferase (RT), respectively. One region of the cDNA (amino acids 325 to 387) showed similarity to ceramide UDP-galactosyltransferases of mice, rats and humans. A crude extract from Escherichia coli, in which the protein was expressed from the cDNA, showed high UF3GaT activity but low UF3GT activity, and was similar in K(m), optimal pH and substrate specificity to UF3GaT from V. mungo. We conclude that we have obtained UDP-galactose:flavonoid 3-O-galactosyltransferase (UF3GaT) cDNA from V. mungo.

  16. A TGACGT motif in the 5'-upstream region of alpha-amylase gene from Vigna mungo is a cis-element for expression in cotyledons of germinated seeds.

    Science.gov (United States)

    Yamauchi, D

    2001-06-01

    Alpha-amylase is expressed at high levels in cotyledons of germinated seeds of Vigna mungo. The mRNA for alpha-amylase appeared in cotyledons of the seeds at 1 d after imbibition started (DAI). Two TGACGT motifs at -445 and at -125 in the promoter region of the gene interacted with nuclear proteins from cotyledons of dry seeds and the activities were detected until 3 DAI. A transient assay with particle bombardment showed that the downstream region from -135 in the promoter was required for high level expression in the cotyledons and the activity was reduced by mutation of the TGACGT motif at -125. The activities to bind the TGACGT motifs were detected in the axes of the seeds at 1 DAI but disappeared at 4 DAI, although the mRNA for alpha-amylase in the axes appeared at 4 DAI and increased in level by 6 DAI. A transient assay experiment showed that a positive regulatory element for the expression in the axes was located in the region from -630 to -453. These results indicated that the TGACGT motif at -125 was required for high level expression of the gene in the cotyledons of the germinated seeds.

  17. Mapping of Mungbean Yellow Mosaic India Virus (MYMIV and powdery mildew resistant gene in black gram [Vigna mungo(L. Hepper

    Directory of Open Access Journals (Sweden)

    Tuba Anjum,K. Sanjeev Gupta and Subhojit Datta

    2010-07-01

    Full Text Available Black gram, one of the important species of Asian Vigna group of grain legumes, is widely grown in South Asia and is animportant source of dietary protein. The two main biological constraints particularly Mungbean Yellow Mosaic IndiaVirus(MYMIV and powdery mildew pose a major threat to black gram production in India. Several reports on mappingmungbean yellow mosaic virus disease and powdery mildew resistant genes on black gram using parental lines suitable forcountries viz. Australia and Japan are available. However, to achieve precision in plant breeding, it is important that mappingof traits are done using parental lines which are best suited for the target area/country. Microsatellite markers facilitateeffective screening of mapping population and marker assisted selection for target traits such as disease resistance in manycrops. Linkage mapping for identification of genes conferring resistance to these target traits in the crop is underway. Theparents selected for MYMIV mapping population are DPU 88-31 as resistant source and AKU 9904 as susceptible one. Forestablishment of powdery mildew mapping population RBU 38 was used as resistant and DPU 88-31 as the susceptible one.Parental polymorphism was assessed using 363 SSR and 24 RGH markers. Efforts are being made to identify the markerstightly linked to the genes responsible for resistance which will be useful for marker assisted breeding for developingMYMIV and powdery mildew resistant cultivars in black gram.

  18. Characterization of a symbiotically effective Rhizobium resistant to arsenic: Isolated from the root nodules of Vigna mungo (L.) Hepper grown in an arsenic-contaminated field.

    Science.gov (United States)

    Mandal, Santi M; Pati, Bikas R; Das, Amit K; Ghosh, Ananta K

    2008-04-01

    Bacteria were isolated from the root nodules of Vigna mungo (L.) Hepper, grown in an arsenic-contaminated field and the strain was selected by its nodulation ability as well as better arsenic tolerant capacity compared to others. The selected strain was identified as Rhizobium by 16S rDNA sequencing and designated as VMA301. Phylogenetic analysis of the gene sequences showed its close relatedness with Sinorhizobium fredii. LC(50) value of arsenate for the bacteria as determined by flow cytometry was found to be 2.8 mM and arsenic uptake was measured by atomic absorption spectrometry as 0.048 mg g(-1) biomass. The high amount of arsenic was toxic to the cell, which changed the morphology of the bacteria to an elongated shape. Presence of a transcriptional regulatory gene (ArsR) of the ars genetic system was confirmed by amplification and sequencing. The symbiotic property of the isolate was also confirmed by amplification and sequencing of the NodC gene. These results indicate that the isolated Rhizobium bacteria may exert dual roles in the environment, arsenic bioremediation from the soil as well as increase of soil fertility through nitrogen fixation.

  19. Detoxification potential and expression analysis of eutypine reducing aldehyde reductase (VrALR) during progressive drought and recovery in Vigna radiata (L.) Wilczek roots.

    Science.gov (United States)

    Sengupta, Debashree; Mudalkar, Shalini; Reddy, Attipalli R

    2012-10-01

    Generation of reactive oxygen species (ROS) in plants is an inevitable consequence of adverse environmental cues and the ability to detoxify deleterious by-products of ROS-mediated oxidation reactions reflect an important defence strategy to combat abiotic stress. Here, we have cloned the eutypine reducing aldehyde reductase gene (VrALR) from Vigna radiata (L.) Wilczek roots. We have expressed and purified the VrALR protein and analyzed its enzyme kinetic parameters and catalytic efficiency with three different substrates to confirm its identity. The functional characterization of this enzyme was unravelled through heterologous expression of the gene in Escherichia coli BL21 and an oxidative stress-sensitive Saccharomyces cerevisiae mutant strain, W3O3-1-A. Finally, the endogenous VrALR enzyme activity and the mRNA expression patterns of the VrALR gene in the roots of V. radiata in response to progressive drought stress in vivo was studied to correlate the ROS-detoxifying role of this important enzyme under the influence of progressive drought stress. Our results, for the first time, demonstrate that eutypine reducing VrALR provides varying degree of stress tolerance in bacteria, yeast systems and also plays a promising protective role against oxidative stress in V. radiata roots during gradual water deprivation. The present study provides an unequivocal evidence to understand the crucial role of aldehyde reductase ROS-detoxifying system which is highly essential for developing stress tolerance in economically important crop plants. PMID:22837052

  20. Impact of pesticides on plant growth promotion of Vigna radiata and non-target microbes: comparison between chemical- and bio-pesticides.

    Science.gov (United States)

    Gupta, Sukriti; Gupta, Rashi; Sharma, Shilpi

    2014-08-01

    To compare the target and non-target effects of two chemical-pesticides (chlorpyrifos and endosulfan) with that of a bio-pesticide (azadirachtin), Vigna radiata (mung bean) was grown in a randomized pot experiment with recommended and higher application rates of pesticides. Colony counts enumerating specific microbial populations, viz. fungi, Pseudomonas, nitrogen-fixing bacteria, and phosphate-solubilizing microorganisms, were performed. In addition, several plant growth parameters such as root and shoot lengths were also monitored. It was observed that the pesticides exerted a suppressive effect on different microbial communities under study in the initial 30 days period. The bacterial and fungal populations in chlorpyrifos treated plants increased thereafter. Endosulfan resulted in enhancement of fungi and nitrogen-fixing bacteria, although phosphate-solubilizing microorganisms were suppressed at higher application rates. Azadirachtin, which is gaining popularity owing to its biological origin, did not result in enhancement of any microbial populations; on the other hand, it had a deleterious effect on phosphate-solubilizing bacteria. This study is the first to evaluate the non-target effects of pesticides with a comparison between chemical- and bio-pesticides, and also stresses the importance of critical investigation of bio-pesticides before their wide spread application in agriculture.

  1. Tillage and residue management effect on soil properties, crop performance and energy relations in greengram (Vigna radiata L. under maize-based cropping systems

    Directory of Open Access Journals (Sweden)

    J.R. Meena

    2015-12-01

    Full Text Available Effect of tillage and crop residue management on soil properties, crop performance, energy relations and economics in greengram (Vigna radiata L. was evaluated under four maize-based cropping systems in an Inceptisol of Delhi, India. Soil bulk density, hydraulic conductivity and aggregation at 0–15 cm layer were significantly affected both by tillage and cropping systems, while zero tillage significantly increased the soil organic carbon content. Yields of greengram were significantly higher in maize–chickpea and maize–mustard systems, more so with residue addition. When no residue was added, conventional tillage required 20% higher energy inputs than the zero tillage, while the residue addition increased the energy output in both tillage practices. Maize–wheat–greengram cropping system involved the maximum energy requirement and the cost of production. However, the largest net return was obtained from the maize–chickpea–greengram system under the conventional tillage with residue incorporation. Although zero tillage resulted in better aggregation, C content and N availability in soil, and reduced the energy inputs, cultivation of summer greengram appeared to be profitable under conventional tillage system with residue incorporation.

  2. Changes in hydraulic conductance cause the difference in growth response to short-term salt stress between salt-tolerant and -sensitive black gram (Vigna mungo) varieties.

    Science.gov (United States)

    Win, Khin Thuzar; Oo, Aung Zaw; Ookawa, Taiichiro; Kanekatsu, Motoki; Hirasawa, Tadashii

    2016-04-01

    Black gram (Vigna mungo) is an important crop in Asia, However, most black gram varieties are salt-sensitive. The causes of varietal differences in salt-induced growth reduction between two black gram varieties, 'U-Taung-2' (salt-tolerant; BT) and 'Mut Pe Khaing To' (salt-sensitive; BS), were examined the potential for the first step toward the genetic improvement of salt tolerance. Seedlings grown in vermiculite irrigated with full-strength Hoagland solution were treated with 0mM NaCl (control) or 225 mM NaCl for up to 10 days. In the 225 mM NaCl treatment, plant growth rate, net assimilation rate, mean leaf area, leaf water potential, and leaf photosynthesis were reduced more in BS than in BT plants. Leaf water potential was closely related to leaf photosynthesis, net assimilation rate, and increase in leaf area. In response to salinity stress, hydraulic conductance of the root, stem, and petiole decreased more strongly in BS than in BT plants. The reduction in stem and petiole hydraulic conductance was caused by cavitation, whereas the reduction in root hydraulic conductance in BS plants was caused by a reduction in root surface area and hydraulic conductivity. We conclude that the different reduction in hydraulic conductance is a cause of the differences in the growth response between the two black gram varieties under short-term salt stress.

  3. Effect of Trichoderma harzianum biomass and Bradyrhizobium sp. strain NC 92 to control leaf blight disease of bambara groundnut (Vigna subterranea caused by Rhizoctonia solani in the field

    Directory of Open Access Journals (Sweden)

    Mana Kanjanamaneesathian

    2007-01-01

    Full Text Available Four hundred and sixty two strains of Trichoderma spp. were isolated from 23 soil samples in which groundnut (Arachis hypogaea L. and bambara groundnut (Vigna subterranea L. had been planted in Songkhla, Phattalung, Nakhon Si Thammarat, Narathiwat and Yala provinces. These fungi were tested against Rhizoctonia solani, a causal agent of leaf blight of bambara groundnut, using dual culture technique on PDA medium. Among 462 isolates tested, 226 isolates had an ability to overgrow R. solani completely. Further testing found 13 isolates having the ability to parasitize mycelia of R. solani. Among these isolates, ThB-1-54 produced a cellulolytic enzyme on congo-red agar. This isolate was later identified as T. harzianum Rifai. In the field test, applying biomass of the isolate ThB-1-54 cultured on ground mesocarp fiber of oil palm, the combination of the isolate ThB-1-54 on ground mesocarp fiber of oil palm and Bradyrhizobium sp. (strain NC 92, or fungicide (iprodione had no effect on disease severity, yield, or the amount of total nitrogen content in stems or seeds of bambara groundnut plant.

  4. Genetic Parameters and Combining Ability Effects of Parents for Seed Yield and other Quantitative Traits in Black Gram [Vigna mungo (L. Hepper

    Directory of Open Access Journals (Sweden)

    Supriyo CHAKRABORTY

    2010-06-01

    Full Text Available Line x tester analysis was carried out in black gram [Vigna mungo (L. Hepper], an edible legume, to estimate the gca (general combining ability effects of parents (3 lines and 3 testers and the SCA (specific combining ability effects of 9 crosses for seed yield and other eleven quantitative traits. Though additive and nonadditive gene actions governed the expression of quantitative traits, the magnitude of nonadditive gene action was higher than that of additive gene action for each quantitative trait. Two parents viz. �UG157� and �DPU915� were good general combiners. Two crosses namely �PDB 88-31�/�DPU 915� and �PLU 277�/�KAU7� had high per se performance along with positive significant SCA effect for seed yield/plant. The degree of dominance revealed overdominance for all the traits except clusters/plant with partial dominance. The predictability ratio also revealed the predominant role of nonadditive gene action in the genetic control of quantitative traits. Narrow sense heritability was also low for each trait. Recurrent selection or biparental mating followed by selection which can exploit both additive and nonadditive gene actions would be of interest for yield improvement in black gram. Due to presence of high magnitude of nonadditive gene action, heterosis breeding could also be attempted to develop low cost hybrid variety using genetic male sterility system in black gram.

  5. Changes in hydraulic conductance cause the difference in growth response to short-term salt stress between salt-tolerant and -sensitive black gram (Vigna mungo) varieties.

    Science.gov (United States)

    Win, Khin Thuzar; Oo, Aung Zaw; Ookawa, Taiichiro; Kanekatsu, Motoki; Hirasawa, Tadashii

    2016-04-01

    Black gram (Vigna mungo) is an important crop in Asia, However, most black gram varieties are salt-sensitive. The causes of varietal differences in salt-induced growth reduction between two black gram varieties, 'U-Taung-2' (salt-tolerant; BT) and 'Mut Pe Khaing To' (salt-sensitive; BS), were examined the potential for the first step toward the genetic improvement of salt tolerance. Seedlings grown in vermiculite irrigated with full-strength Hoagland solution were treated with 0mM NaCl (control) or 225 mM NaCl for up to 10 days. In the 225 mM NaCl treatment, plant growth rate, net assimilation rate, mean leaf area, leaf water potential, and leaf photosynthesis were reduced more in BS than in BT plants. Leaf water potential was closely related to leaf photosynthesis, net assimilation rate, and increase in leaf area. In response to salinity stress, hydraulic conductance of the root, stem, and petiole decreased more strongly in BS than in BT plants. The reduction in stem and petiole hydraulic conductance was caused by cavitation, whereas the reduction in root hydraulic conductance in BS plants was caused by a reduction in root surface area and hydraulic conductivity. We conclude that the different reduction in hydraulic conductance is a cause of the differences in the growth response between the two black gram varieties under short-term salt stress. PMID:26962708

  6. A facile biomimetic preparation of highly stabilized silver nanoparticles derived from seed extract of Vigna radiata and evaluation of their antibacterial activity

    Science.gov (United States)

    Choudhary, Manoj Kumar; Kataria, Jyoti; Cameotra, Swaranjit Singh; Singh, Jagdish

    2016-01-01

    The significant antibacterial activity of silver nanoparticles draws the major attention toward the present nanobiotechnology. Also, the use of plant material for the synthesis of metal nanoparticles is considered as a green technology. In this context, a non-toxic, eco-friendly, and cost-effective method has been developed for the synthesis of silver nanoparticles using seed extract of mung beans ( Vigna radiata). The synthesized nanoparticles have been characterized by UV-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), atomic absorption spectroscopy (AAS), and X-ray diffraction (XRD). The UV-visible spectrum showed an absorption peak at around 440 nm. The different types of phytochemicals present in the seed extract synergistically reduce the Ag metal ions, as each phytochemical is unique in terms of its structure and antioxidant function. The colloidal silver nanoparticles were observed to be highly stable, even after 5 months. XRD analysis showed that the silver nanoparticles are crystalline in nature with face-centered cubic geometry and the TEM micrographs showed spherical particles with an average size of 18 nm. Further, the antibacterial activity of silver nanoparticles was evaluated by well-diffusion method and it was observed that the biogenic silver nanoparticles have an effective antibacterial activity against Escherichia coli and Staphylococcus aureus. The outcome of this study could be useful for nanotechnology-based biomedical applications.

  7. Characterization of VuMATE1 expression in response to iron nutrition and aluminum stress reveals adaptation of rice bean (Vigna umbellata to acid soils through cis regulation

    Directory of Open Access Journals (Sweden)

    Meiya eLiu

    2016-04-01

    Full Text Available Rice bean (Vigna umbellata VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of rice bean to acidic soils where Al stress imposed but Fe deficiency pressure released.

  8. Polyamines Confer Salt Tolerance in Mung Bean (Vigna radiata L.) by Reducing Sodium Uptake, Improving Nutrient Homeostasis, Antioxidant Defense, and Methylglyoxal Detoxification Systems

    Science.gov (United States)

    Nahar, Kamrun; Hasanuzzaman, Mirza; Rahman, Anisur; Alam, Md. Mahabub; Mahmud, Jubayer-Al; Suzuki, Toshisada; Fujita, Masayuki

    2016-01-01

    The physiological roles of PAs (putrescine, spermidine, and spermine) were investigated for their ability to confer salt tolerance (200 mM NaCl, 48 h) in mung bean seedlings (Vigna radiata L. cv. BARI Mung-2). Salt stress resulted in Na toxicity, decreased K, Ca, Mg, and Zn contents in roots and shoots, and disrupted antioxidant defense system which caused oxidative damage as indicated by increased lipid peroxidation, H2O2 content, O2•- generation rate, and lipoxygenase activity. Salinity-induced methylglyoxal (MG) toxicity was also clearly evident. Salinity decreased leaf chlorophyll (chl) and relative water content (RWC). Supplementation of salt affected seedlings with exogenous PAs enhanced the contents of glutathione and ascorbate, increased activities of antioxidant enzymes (dehydroascorbate reductase, glutathione reductase, catalase, and glutathione peroxidase) and glyoxalase enzyme (glyoxalase II), which reduced salt-induced oxidative stress and MG toxicity, respectively. Exogenous PAs reduced cellular Na content and maintained nutrient homeostasis and modulated endogenous PAs levels in salt affected mung bean seedlings. The overall salt tolerance was reflected through improved tissue water and chl content, and better seedling growth. PMID:27516763

  9. 壳聚糖对长豆角品质和产量的影响%Effects of Chitosan on the Quality and Yield of Vigna sinensis(L. ) Savi

    Institute of Scientific and Technical Information of China (English)

    吴黄铭; 陈惠萍

    2011-01-01

    [目的]为生产长豆角无公害寻找有效的生长调节剂.[方法]在长豆角现蕾前,采用1.2和1.6 mg/ml壳聚糖溶液喷洒长豆角植株,研究其豆荚品质和产量的变化.[结果]壳聚糖有效减少了长豆角虫害的发生,提高了长豆角豆荚可溶性蛋白含量、可溶性糖含量和维生素C含量,降低粗纤维含量,并增加了单果重量、单株结荚数和单位面积产量.[结论]壳聚糖作为一种植物生长调节荆,显著增加作物产量和改善作物营养品质.%[ Objective] The aim was to search for an effective growth regulator for producing non-pollution Vigna sinensis(L. ) Savi. [ Method] Before theirs flower buds appearing, the plants of Vigna sinensis( L. ) Savi were sprayed with 1.2 and 1.6 mg/ml of chitosan respectively. Then, .the changes of their fruit ,s qualties and yields were studied. [ Result] Chitosan could reduce effectively the occurrences of insect pest,and increase significantly the contents of soluble protein, soluble sugar, vitamin C in the fruits of Vigna sinensis ( L. ) Savi, whereas decrease the content of curde fiber. Furthermore chitosan increased the single fruit, weight, single form pods, number and unit area output. [ Conclusion] Chitosan as a growth regulator of plant, markly raises the yield and improve the qualtiy in crop.

  10. Productivity and residual benefits of grain legumes to sorghum under semi-arid conditions in southwestern Zimbabwe

    NARCIS (Netherlands)

    Ncube, B.; Twomlow, S.J.; Wijk, van M.T.; Dimes, J.P.; Giller, K.E.

    2007-01-01

    The productivity and residual benefits of four grain legumes to sorghum (Sorghum bicolor) grown in rotation were measured under semi-arid conditions over three cropping seasons. Two varieties of each of the grain legumes; cowpea (Vigna unguiculata); groundnut (Arachis hypogaea); pigeon pea (Cajanus

  11. Dicty_cDB: Contig-U03703-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1026 ) VUH2-89O22TV VUUBBa (VUH2) Vigna unguiculata geno... 34 0.94 2 ( AY712586 ) Zaluzianskya microsiphon ...s... 44 1.3 1 ( EU004203 ) Saccharomyces cerevisiae YJM789 mitochondrion, co... 44 1.3 1 ( AY712590 ) Zaluzi...anskya angustifolia rpl16 gene, intron; chl... 44 1.3 1 ( AY712589 ) Zaluzianskya

  12. Traditional African plant products to protect stored cowpeas against insect damage : the battle against the beetle

    NARCIS (Netherlands)

    Boeke, S.J.

    2002-01-01

    Seeds of the cowpea plant, Vigna unguiculata , a tropical crop, are very susceptible to attack by the cowpea beetle. This specialist beetle needs only the beans to reproduce rapidly.Most farmers in West Africa have few possibilities to treat the beans and they face their stored supply

  13. Cowpea N rhizodeposition and its below-ground transfer to a co-existing and to a subsequent millet crop on a sandy soil of the Sudano-Sahelian eco-zone

    DEFF Research Database (Denmark)

    Laberge, Guillaume; Haussmann, Bettina I.G.; Ambus, Per;

    2011-01-01

    Nitrogen (N) rhizodeposition by cowpea (Vigna unguiculata (L.) Walp) is potentially a large N source in cropping systems of Sub-Saharan Africa. A field experiment was conducted to measure cowpea N rhizodeposition under the conditions of the Sudano-Sahelian zone using direct 15N labelling techniques...

  14. Cover crops and organic mulches for nematode, weed, and plant health management

    Science.gov (United States)

    Two field experiments were conducted in 2003 and 2004 to evaluate ‘Tropic Sun’ sunn hemp (Crotalaria juncea) and ‘Iron Clay’ cowpea (Vigna unguiculata) as summer cover crops and as organic mulches. Both experiments were a 3 x 3 split-plot design in which the main plots were summer planting of sunn h...

  15. Archaeophytopathology of Global Soybean Rust

    Science.gov (United States)

    Phakopsora pachyrhizi and P. meibomiae are two rust species that infect soybean (Glycine max). A number of other hosts support the uredinial growth of these Phakopsora, including Pachyrhizus erosus, Pueraria lobata, and Vigna unguiculata, but no aecial host is known. Traditionally, these two species...

  16. US-1136, US-1137, and US-1138 Cowpea Germplasm Lines for Use as a Cover Crop

    Science.gov (United States)

    The adoption of sustainable and organic cultural practices in recent years has resulted in an increased use of cover crops. Cowpea (Vigna unguiculata L.) is an excellent warm season cover crop due to its tolerance of heat and drought stress, ability to grow well in sandy, poor, acidic soils, high b...

  17. Utilization of summer legumes as bioenergy feedstocks

    Science.gov (United States)

    Sunn hemp (Crotolaria juncea), is a fast growing, high biomass yielding tropical legume that may be a possible southeastern bioenergy crop. When comparing this legume to a commonly grown summer legume—cowpeas (Vigna unguiculata), sunn hemp was superior in biomass yield and subsequent energy yield. S...

  18. Yield performance of cowpea plant introductions grown in calcareous soils

    Science.gov (United States)

    Cowpea or Southernpea [Vigna unguiculata (L.) Walp.] is an important legume crop used as a feed for livestock, as a green vegetable and for consumption of its dry beans which provide 22-25% protein. The crop is very sensitive to alkaline soil conditions. When grown at a soil pH of 7.5 or higher, co...

  19. Common bean and cowpea improvement in Angola

    Science.gov (United States)

    During 2014 and 2015, the Instituto de Investigação Agronómica (IIA) evaluated the performance of common bean (Phaselolus vulgaris L.) breeding lines and improved cowpea (Vigna unguiculata L. Walp.) varieties. The field experiments were planted in the lowlands at Mazozo and in the highlands at Chian...

  20. Separating multiple, short-term deleterious effects of saline solutions to the growth of cowpea seedlings

    Science.gov (United States)

    Reductions in plant growth due to salinity are of global importance in natural and agricultural landscapes. Short-term (48 h) solution culture experiments studied 404 treatments with seedlings of cowpea (Vigna unguiculata (L.) Walp. cv. Caloona) to examine the multiple deleterious effects of Ca, Mg...

  1. Grain legume impacts on soil biological processes in Sub-Saharan Africa

    Science.gov (United States)

    Grain legumes occupy about 20 million hectares in Africa. The major crops are cowpea (Vigna unguiculata), which is grown on about 11 million hectares mostly in west Africa, and common bean (Phaseolus vulgaris), grown on about 5 million hectares mostly in eastern and southern Africa. These grain le...

  2. Virus Survey of Commercial Cowpea Fields in the U. S. and Evaluation of the Core of the USDA Cowpea Germplasm Collection for Resistance to Tomato Spotted Wilt Virus

    Science.gov (United States)

    Cowpea (Vigna unguiculata) is an important vegetable crop in the southern United States. Cowpea plants are susceptible to over 20 viruses and many of them are seed-borne. A survey was carried out to evaluate the current virus status in commercial cowpea fields in five States (MO, NM, SC, TN and TX...

  3. Evaluation of grapevine as a host for the glassy-winged sharpshooter

    Science.gov (United States)

    Grapevine was evaluated as a feeding and oviposition host for the glassy-winged sharpshooter. Two sets of experiments were conducted. The first set compared performance and preference of glassy-winged sharpshooter females for grapevine (cv. Chardonnay) versus cowpea (Vigna unguiculata cultivar black...

  4. Invloed van ras, mulch en bemesting op de productie en het saldo van sopropo en kouseband : verslag van een proef te Saramacca, Suriname

    NARCIS (Netherlands)

    Putter, de H.; Wongsonadi, H.

    2010-01-01

    In 2010 werd een proef met sopropo (Momordica charanti) en een proef met kouseband (Vigna unguiculata sesquipedalis) uitgevoerd bij een teler in Suriname in de regio Saramacca. Doel was om het effect van variëteit, toepassing van mulch en bemestingstrategie op de productie en op het gewassaldo vast

  5. Seed coat darkening in Cowpea bean

    Science.gov (United States)

    Seed coat of cowpea bean (Vigna unguiculata L. Walp) slowly browns to a darker color during storage. High temperature and humidity during storage might contribute to this color change. Variation in browning rate among seeds in a lot leads to a mixture of seed colors creating an unacceptable product...

  6. Field screening of cowpea cultivars for alkaline soil tolerance

    Science.gov (United States)

    Cowpea or Southernpea [Vigna unguiculata (L.) Walp.] is an important legume crop used as a feed for livestock, as a green vegetable and for consumption of its dry beans which provide 22-25% protein. The crop is very sensitive to alkaline soil conditions. When grown at soil pH of 7.5 or higher, cowp...

  7. Field Performance of Cowpea Genotypes Grown under Virus Pressure in Puerto Rico

    Science.gov (United States)

    Cowpea [Vigna unguiculata (L.) Walp.] is an important grain legume in many regions of the tropics. However, viral diseases, particularly Cucumber mosaic virus (CMV) and Blackeye cowpea mosaic virus (BlCMV), can be a limiting factor in cowpea production. We evaluated in replicated field plots and un...

  8. Yield performance of cowpea genotypes grown in alkaline soils

    Science.gov (United States)

    Cowpea or Southernpea [Vigna unguiculata (L.) Walp.] is an important legume crop used as a feed for livestock, as a green vegetable and for consumption of its dry beans which provide 22-25% protein. The crop is very sensitive to alkaline soil conditions. When grown at soil pH of 7.5 or higher, cowp...

  9. RNA-dependent RNA polymerases from cowpea mosaic virus-infected cowpea leaves

    NARCIS (Netherlands)

    Dorssers, L.C.J.

    1983-01-01

    The aim of the research described in this thesis was the purification and identification of the RNA-dependent RNA polymerase engaged in replicating viral RNA in cowpea mosaic virus (CPMV)- infected cowpea leaves.Previously, an RNA-dependent RNA polymerase produced upon infection of Vigna unguiculata

  10. Dicty_cDB: Contig-U04537-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available . 44 7.3 1 ( FF388493 ) MOOC791TF MOO Vigna unguiculata cDNA 5', mRNA seq... 44 7.3 1 ( AW332197 ) S5E1 AGS-...5' en... 44 7.3 1 ( FM250331 ) Caenorhabditis elegans EST, clone C02_ce5.trans...

  11. Purification and properties of cowpea mosaic virus RNA replicase

    NARCIS (Netherlands)

    Zabel, P.

    1978-01-01

    This thesis concerns the partial purification and properties of an RNA-dependent RNA polymerase (RNA replicase) produced upon infection of Vigna unguiculata plants with Cowpea Mosaic Virus (CPMV). The enzyme is believed to be coded, at least in part, by the virus genome and to be responsible for the

  12. 外源Cd、Cd - Zn复合污染对绿豆苗的影响%Effects of exogenous cadmium and zinc joined pollution on the vigna radiate

    Institute of Scientific and Technical Information of China (English)

    陈坤; 徐龙君; 袁智; 李世贵; 易俊

    2011-01-01

    The paper is aimed at presenting our pot-planting experiment results on the study of the effects of exogenous cadmium and zinc joined pollution on the vigna. Our experiments were done to investigate the effects of various concentrations of exogenous Cd, (singly polluted and Zn + Cd (20 mg/kg) combined-polluted) on the vigna radiated plant height and absorption of Cd in the two soils respectively. The results of our experiments show that under the condition of exogenous Cd single pollution, the height of vigna radiated plant tends to decrease significantly compared with the contrast group when the growing time is ruled as 7 days. However, compared with the blank group, vigna radiated height tends to decrease by 11.29%, 12.20%, 14.57%, 21.24%, 15.38% and 21.24%, respectively. With the increase of Cd pollution, its height growth was also restrained , but with the increase of the growing time the harm would be declined. In addition, with the increase of the Cd pollution, the Cd content in vigna radiate would increase in positive correlation with Cd pollution. At the same level of Cd pollution, it proved that the Cd content in the vigna radiate of 7 days tends to be higher than that of 3 days. To be exact, at different pollution levels, the plant height in soil sample 1 was found to be taller than that of in soil sample 2 whereas Cd content in vigna radiate body growing in soil sample 1 turned to be smaller than that of in soil sample 2. In the condition of Zn+Cd (20 mg/kg) combined pollution, the plant height influence turned to be lower than the blank group. Moreover, when Cd content in the vigna radiate body increased with the increase of Zn's concentration, a positive correlation can be found in our experiments, indicating a synergistic behavior in the growth. Thus, comparing soil sample 1 and soil sample 2, it can be found that the plant height in soil sample 2 would be shorter, but, in soil sample 1, Cd content in vigna radiate tends to grow taller than that of in

  13. Influence of sodium chloride on the regulation of Krebs cycle intermediates and enzymes of respiratory chain in mungbean (Vigna radiata L. Wilczek) seedlings.

    Science.gov (United States)

    Saha, Papiya; Kunda, Pranamita; Biswas, Asok K

    2012-11-01

    The effect of common salt (NaCl) on ion contents, Krebs cycle intermediates and its regulatory enzymes was investigated in growing mungbean (Vigna radiata L. Wilczek, B 105) seedlings. Sodium and chloride ion contents increased in both root and shoot whereas potassium ion content decreased in shoot of test seedlings with increasing concentrations of NaCl. Organic acids like pyruvate and citrate levels increased whereas malate level decreased under stress in both roots and shoots. Salt stress also variedly affected the activities of different enzymes of respiratory chain. The activity of pyruvate dehydrogenase (E.C. 1.2.4.1) decreased in 50 mM NaCl but increased in 100 mM and 150 mM concentrations, in both root and shoot samples. Succinate dehydrogenase (E.C. 1.3.5.1) activity was reduced in root whereas stimulated in shoot under increasing concentrations of salt. The activity of isocitrate dehydrogenase (E.C. 1.1.1.41) and malate dehydrogenase (E.C. 1.1.1.37) decreased in both root and shoot samples under salt stress. On the contrary, pretreatment of mungbean seeds with sublethal dose of NaCl was able to overcome the adverse effects of stress imposed by NaCl to variable extents with significant alterations of all the tested parameters, resulting in better growth and efficient respiration in mungbean seedlings. Thus, plants can acclimate to lethal level of salinity by pretreatment of seeds with sublethal level of NaCl, which serves to improve their health and production under saline condition, but the sublethal concentration of NaCl should be carefully chosen.

  14. Transcriptional Slippage and RNA Editing Increase the Diversity of Transcripts in Chloroplasts: Insight from Deep Sequencing of Vigna radiata Genome and Transcriptome.

    Directory of Open Access Journals (Sweden)

    Ching-Ping Lin

    Full Text Available We performed deep sequencing of the nuclear and organellar genomes of three mungbean genotypes: Vigna radiata ssp. sublobata TC1966, V. radiata var. radiata NM92 and the recombinant inbred line RIL59 derived from a cross between TC1966 and NM92. Moreover, we performed deep sequencing of the RIL59 transcriptome to investigate transcript variability. The mungbean chloroplast genome has a quadripartite structure including a pair of inverted repeats separated by two single copy regions. A total of 213 simple sequence repeats were identified in the chloroplast genomes of NM92 and RIL59; 78 single nucleotide variants and nine indels were discovered in comparing the chloroplast genomes of TC1966 and NM92. Analysis of the mungbean chloroplast transcriptome revealed mRNAs that were affected by transcriptional slippage and RNA editing. Transcriptional slippage frequency was positively correlated with the length of simple sequence repeats of the mungbean chloroplast genome (R2=0.9911. In total, 41 C-to-U editing sites were found in 23 chloroplast genes and in one intergenic spacer. No editing site that swapped U to C was found. A combination of bioinformatics and experimental methods revealed that the plastid-encoded RNA polymerase-transcribed genes psbF and ndhA are affected by transcriptional slippage in mungbean and in main lineages of land plants, including three dicots (Glycine max, Brassica rapa, and Nicotiana tabacum, two monocots (Oryza sativa and Zea mays, two gymnosperms (Pinus taeda and Ginkgo biloba and one moss (Physcomitrella patens. Transcript analysis of the rps2 gene showed that transcriptional slippage could affect transcripts at single sequence repeat regions with poly-A runs. It showed that transcriptional slippage together with incomplete RNA editing may cause sequence diversity of transcripts in chloroplasts of land plants.

  15. Transcript dynamics at early stages of molecular interactions of MYMIV with resistant and susceptible genotypes of the leguminous host, Vigna mungo.

    Science.gov (United States)

    Kundu, Anirban; Patel, Anju; Paul, Sujay; Pal, Amita

    2015-01-01

    Initial phases of the MYMIV-Vigna mungo interaction is crucial in determining the infection phenotype upon challenging with the virus. During incompatible interaction, the plant deploys multiple stratagems that include extensive transcriptional alterations defying the virulence factors of the pathogen. Such molecular events are not frequently addressed by genomic tools. In order to obtain a critical insight to unravel how V. mungo respond to Mungbean yellow mosaic India virus (MYMIV), we have employed the PCR based suppression subtractive hybridization technique to identify genes that exhibit altered expressions. Dynamics of 345 candidate genes are illustrated that differentially expressed either in compatible or incompatible reactions and their possible biological and cellular functions are predicted. The MYMIV-induced physiological aspects of the resistant host include reactive oxygen species generation, induction of Ca2+ mediated signaling, enhanced expression of transcripts involved in phenylpropanoid and ubiquitin-proteasomal pathways; all these together confer resistance against the invader. Elicitation of genes implicated in salicylic acid (SA) pathway suggests that immune response is under the regulation of SA signaling. A significant fraction of modulated transcripts are of unknown function indicating participation of novel candidate genes in restricting this viral pathogen. Susceptibility on the other hand, as exhibited by V. mungo Cv. T9 is perhaps due to the poor execution of these transcript modulation exhibiting remarkable repression of photosynthesis related genes resulting in chlorosis of leaves followed by penalty in crop yield. Thus, the present findings revealed an insight on the molecular warfare during host-virus interaction suggesting plausible signaling mechanisms and key biochemical pathways overriding MYMIV invasion in resistant genotype of V. mungo. In addition to inflate the existing knowledge base, the genomic resources identified in

  16. Transcript dynamics at early stages of molecular interactions of MYMIV with resistant and susceptible genotypes of the leguminous host, Vigna mungo.

    Directory of Open Access Journals (Sweden)

    Anirban Kundu

    Full Text Available Initial phases of the MYMIV-Vigna mungo interaction is crucial in determining the infection phenotype upon challenging with the virus. During incompatible interaction, the plant deploys multiple stratagems that include extensive transcriptional alterations defying the virulence factors of the pathogen. Such molecular events are not frequently addressed by genomic tools. In order to obtain a critical insight to unravel how V. mungo respond to Mungbean yellow mosaic India virus (MYMIV, we have employed the PCR based suppression subtractive hybridization technique to identify genes that exhibit altered expressions. Dynamics of 345 candidate genes are illustrated that differentially expressed either in compatible or incompatible reactions and their possible biological and cellular functions are predicted. The MYMIV-induced physiological aspects of the resistant host include reactive oxygen species generation, induction of Ca2+ mediated signaling, enhanced expression of transcripts involved in phenylpropanoid and ubiquitin-proteasomal pathways; all these together confer resistance against the invader. Elicitation of genes implicated in salicylic acid (SA pathway suggests that immune response is under the regulation of SA signaling. A significant fraction of modulated transcripts are of unknown function indicating participation of novel candidate genes in restricting this viral pathogen. Susceptibility on the other hand, as exhibited by V. mungo Cv. T9 is perhaps due to the poor execution of these transcript modulation exhibiting remarkable repression of photosynthesis related genes resulting in chlorosis of leaves followed by penalty in crop yield. Thus, the present findings revealed an insight on the molecular warfare during host-virus interaction suggesting plausible signaling mechanisms and key biochemical pathways overriding MYMIV invasion in resistant genotype of V. mungo. In addition to inflate the existing knowledge base, the genomic resources

  17. Transcriptome Profiling Identifies Candidate Genes Associated with the Accumulation of Distinct Sulfur γ-Glutamyl Dipeptides in Phaseolus vulgaris and Vigna mungo Seeds.

    Science.gov (United States)

    Liao, Dengqun; Cram, Dustin; Sharpe, Andrew G; Marsolais, Frédéric

    2013-01-01

    Common bean (Phaseolus vulgaris) and black gram (Vigna mungo) accumulate γ-Glutamyl-S-methylcysteine and γ-Glutamyl-methionine in seed, respectively. Transcripts were profiled by 454 pyrosequencing data at a similar developmental stage coinciding with the beginning of the accumulation of these metabolites. Expressed sequence tags were assembled into Unigenes, which were assigned to specific genes in the early release chromosomal assembly of the P. vulgaris genome. Genes involved in multiple sulfur metabolic processes were expressed in both species. Expression of Sultr3 members was predominant in P. vulgaris, whereas expression of Sultr5 members predominated in V. mungo. Expression of the cytosolic SERAT1;1 and -1;2 was approximately fourfold higher in P. vulgaris while expression of the plastidic SERAT2;1 was twofold higher in V. mungo. Among BSAS family members, BSAS4;1, encoding a cytosolic cysteine desulfhydrase, and BSAS1;1, encoding a cytosolic O-acetylserine sulphydrylase were most highly expressed in both species. This was followed by BSAS3;1 encoding a plastidic β-cyanoalanine synthase which was more highly expressed by 10-fold in P. vulgaris. The data identify BSAS3;1 as a candidate enzyme for the biosynthesis of S-methylcysteine through the use of methanethiol as substrate instead of cyanide. Expression of GLC1 would provide a complete sequence leading to the biosynthesis of γ-Glutamyl-S-methylcysteine in plastids. The detection of S-methylhomoglutathione in P. vulgaris suggested that homoglutathione synthetase may accept, to some extent, γ-Glutamyl-S-methylcysteine as substrate, which might lead to the formation of S-methylated phytochelatins. In conclusion, 454 sequencing was effective at revealing differences in the expression of sulfur metabolic genes, providing information on candidate genes for the biosynthesis of distinct sulfur amino acid γ-Glutamyl dipeptides between P. vulgaris and V. mungo.

  18. Effect of foliar spray from seaweed liquid fertilizer of Ulva reticulata (Forsk.) on Vigna mungo L. and their elemental composition using SEM- energy dispersive spectroscopic analysis

    Institute of Scientific and Technical Information of China (English)

    G Ganapathy Selvam; K Sivakumar

    2013-01-01

    Objective: To identify the effect of seaweed liquied fertilizer (SLF) of Ulva reticulata, as biochemical characteristics of Vigna mungo as well as leaf morphometric analysis such as epidermal and stomata cell variation and distribution of minerals in the leaf. Methods:Experiments were conducted on black gram to study the potential green alga of Ulva reticulata as a biofertilizer. The seeds were sown in soil and SLF were added to soil bed in five different concentrations separately (1%, 2%, 4%, 6% and 8% w/v). Results: Seaweed extract was applied as a foliar spray, the SLF treated plants show maximum growth in 2% of SLF among the various experimental concentrations as well as control. Biochemical profiles like chlorophyll a and b, protein, sugar and starch were found to be higher at 2%. A significant increase in the number of epidermal and stomata cells were observed in 2% SLF treated plants. Whereas at higher concentrations of SLF such as 4%, 6%, and 8% the values of all the parameters were significantly decreased than in the control group. Further the leaf of 2% SLF treated V. mungo have subjected to Scanning Electron Microscopy with Energy Dispersive Spectroscopic analysis it reveals that thepresence of ten elements in the following order: Ca>P>N>Na>K>Mg>Mn>S>Fe>Zn in treated and Ca>N>P>Na>Mg>Mn>K>Zn>S>Fe in control plant. The data generated from study reveal that SLF of U. reticulatea could be used as foliar spray at low concentration of 2% to maximize the growth and yield of V. mungo and also increase the number of stomata in the leaf. Conclusion:The main objective of study result would be the manorial requirement for organic forming and serve as a cost effective ecofriendliness for sustainable agriculture and environment.

  19. Allergic manifestation by black gram (Vigna mungo) proteins in allergic patients, BALB/c mice and RBL-2H3 cells.

    Science.gov (United States)

    Verma, Alok Kumar; Kumar, Sandeep; Sharma, Akanksha; Kumar, Dinesh; Roy, Ruchi; Gupta, Rinkesh Kumar; Chaudhari, Bhushan P; Giridhar, B H; Das, Mukul; Dwivedi, Premendra D

    2014-11-01

    The prevalence of black gram (Vigna mungo) induced allergic reactions are reported from several parts of the world including Asia and Australia. But, a thorough exploration of the allergic reactions induced by black gram proteins is still lacking. Therefore, efforts have been made to explore black gram allergy using in vivo and in vitro approaches. In this study, Simulated Gastric Fluid (SGF) assay and IgE immunoblotting were carried out to identify clinically relevant allergens of black gram. BALB/c mice and RBL-2H3 cells were used for elucidation of allergenic reactions of black gram proteins. Further, this study was extended to screen black gram sensitive patients among nasobronchial allergic patients on the basis of clinical history, skin prick test (SPT), specific IgE levels and IgE immunoblotting. Enhanced levels of specific IgE, IgG1/IgG2a (p < 0.05), histamine (p < 0.05), clinical symptoms, pathological indications in the lungs, intestine and spleen were evident in black gram sensitized BALB/c mice. Moreover, the expression of Th2 cytokine transcripts and GATA-3/T-bet ratio was found enhanced in the treated group. In vitro studies on RBL-2H3 cells,showed increased release of β-hexosaminidase (p < 0.05), histamine (p < 0.05), cysteinyl leukotriene (p<0.05) and prostaglandin D2 (p < 0.05). Further, 8.5% of screened patients were found allergic to black gram and concomitant sensitization with other allergens has shown the possibility of further enhancement in allergenic problem. Conclusively, the present study suggested that black gram consumption may be responsible for inducing immediate type of allergic sensitization in susceptible subjects.

  20. Transcriptome profiling identifies candidate genes associated with the accumulation of distinct γ-glutamyl sulphur dipeptides in Phaseolus vulgaris and Vigna mungo seeds

    Directory of Open Access Journals (Sweden)

    Dengqun eLiao

    2013-03-01

    Full Text Available Common bean (Phaseolus vulgaris and black gram (Vigna mungo accumulate γ-glutamyl-S-methylcysteine and γ-glutamyl-methionine in seed, respectively. Transcripts were profiled by 454 pyrosequencing at a similar developmental stage coinciding with the beginning of the accumulation of these metabolites. Expressed sequence tags were assembled into Unigenes, which were assigned to specific genes in the early release chromosomal assembly of the P. vulgaris genome. Genes involved in multiple sulphur metabolic processes were expressed in both species. Expression of Sultr3 members was predominant in P. vulgaris, whereas expression of Sultr5 members predominated in V. mungo. Expression of the cytosolic SERAT1;1 and -1;2 was approximately four-fold higher in P. vulgaris while expression of the plastidic SERAT2;1 was two-fold higher in V. mungo. Among BSAS family members, BSAS4;1, encoding a cytosolic cysteine desulphydrase, and BSAS1;1, encoding a cytosolic O-acetylserine sulphydrylase were most highly expressed in both species. This was followed by BSAS3;1 encoding a plastidic β-cyanoalanine synthase which was more highly expressed by 10-fold in P. vulgaris. The data identify BSAS3;1 as a candidate enzyme for the biosynthesis of S-methyl-cysteine through the use of methanethiol as substrate instead of cyanide. Expression of GLC1 would provide a complete sequence leading to the biosynthesis of γ-glutamyl-S-methylcysteine in plastids. The detection of S-methylhomoglutathione in P. vulgaris suggested that homoglutathione synthetase may accept, to some extent, γ-glutamyl-S-methylcysteine as substrate, which might lead to the formation of S-methylated phytochelatins. In conclusion, 454 sequencing was effective at revealing differences in the expression of sulphur metabolic genes, providing information on candidate genes for the biosynthesis of distinct sulphur amino acid γ-glutamyl dipeptides between P. vulgaris and V. mungo.

  1. Biochemical and physiological characteristics of tropical mung bean (Vigna radiata L.) cultivars against chronic ozone stress: an insight to cultivar-specific response.

    Science.gov (United States)

    Mishra, Amit Kumar; Agrawal, S B

    2015-05-01

    Surface-level ozone (O3) has been regarded as one of the most significant phytotoxic pollutants worldwide. Investigations addressing adverse impacts of elevated O3 on mung bean (Vigna radiata L.), an important leguminous crop of the Indian subcontinent, are still limited. The present study analyzed the differences on the foliar injury, reactive oxygen species (ROS) generation, antioxidative defense system, physiology, and foliar protein profile of two tropical mung bean cultivars (HUM-2 and HUM-6) exposed to elevated O3 under near-natural conditions. Both cultivars were negatively affected by the pollutant, but the response was cultivar-specific. Results revealed that elevated O3 induced higher levels of ROS (O2 (·-) and H2O2) and lipid peroxidation leading to greater foliar injury in HUM-2 compared to HUM-6. Photosynthetic pigments, photosynthetic rate, stomatal conductance, and photochemical efficiency reduced under elevated O3 exposure and the extent of reduction was higher in HUM-2. Principal component analysis revealed that photosynthetic performance and quantum yield were drastically affected in HUM-2 as compared to HUM-6. Activities of antioxidative enzymes were also stimulated, suggesting generation of oxidative stress under elevated O3. HUM-6 showed higher induction of antioxidative enzymes than HUM-2. One-dimensional gel electrophoresis analysis showed drastic reductions in the abundantly present ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) large and small subunits and the decrease was higher in HUM-2. Altogether, results suggested that higher accumulation of ROS and limited induction of antioxidant defense system led to more leaf injury and impairment of photosynthesis in HUM-2 than HUM-6 depicting its higher sensitivity towards elevated O3.

  2. Development of an efficient in vitro plant regeneration system amenable to Agrobacterium- mediated transformation of a recalcitrant grain legume blackgram (Vigna mungo L. Hepper).

    Science.gov (United States)

    Sainger, Manish; Chaudhary, Darshna; Dahiya, Savita; Jaiwal, Ranjana; Jaiwal, Pawan K

    2015-10-01

    An efficient, rapid and direct multiple shoot regeneration system amenable to Agrobacterium-mediated transformation from primary leaf with intact petiole of blackgram (Vigna mungo) is established for the first time. The effect of the explant type and its age, type and concentration of cytokinin and auxin either alone or in combination and genotype on multiple shoot regeneration efficiency and frequency was optimized. The primary leaf explants with petiole excised from 4-day-old seedlings directly developed multiple shoots (an average of 10 shoots/ explant) from the cut ends of the petiole in 95 % of the cultures on MSB (MS salts and B5 vitamins) medium containing 1.0 μM 6-benzylaminopurine. Elongated (2-3 cm) shoots were rooted on MSB medium with 2.5 μM indole-butyric acid and resulted plantlets were hardened and established in soil, where they resumed growth and reached maturity with normal seed set. The regenerated plants were morphologically similar to seed-raised plants and required 8 weeks time from initiation of culture to establish them in soil. The regeneration competent cells present at the cut ends of petiole are fully exposed and are, thus, easily accessible to Agrobacterium, making this plant regeneration protocol amenable for the production of transgenic plants. The protocol was further successfully used to develop fertile transgenic plants of blackgram using Agrobacterium tumefaciens strain EHA 105 carrying a binary vector pCAMBIA2301 that contains a neomycin phosphotransferase gene (nptII) and a β-glucuronidase (GUS) gene (uidA) interrupted with an intron. The presence and integration of transgenes in putative T0 plants were confirmed by polymerase chain reaction (PCR) and Southern blot hybridization, respectively. The transgenes were inherited in Mendelian fashion in T1 progeny and a transformation frequency of 1.3 % was obtained. This protocol can be effectively used for transferring new traits in blackgram and other legumes for their

  3. Tamaño de Parcela Experimental y Número de Repeticiones para Ensayos de Rendimiento con Caupi, Vigna ungiculata (l Walp

    Directory of Open Access Journals (Sweden)

    Escobar Soto Carlos

    1990-09-01

    Full Text Available La presente investigación se realizó con el propósito de estimar el tamaño óptimo de parcela experimental y el número de repeticiones para ensayos de rendimiento con caupl Vigna ungiculata (L Walp. El ensayo de campo, ensayo en blanco de 20 surcos de 25 m cada uno, se sembró durante el segundo semestre de 1990, en el Centro de Investigación Cotové, municipio de Santa Fe de Antioquia, propiedad de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Colombia, Seccional Medellín. La zona de vida corresponde a la de bosque seco tropical (bs -T. Se sembró la variedad experimental Licatur-1 OCA Calamar, a 0,60 m entre surcos y 0,10 m entre plantas dentro de surcos. La unidad básica se consideró de un surco de un metro de longitud. Para la estimación del tamaño óptimo de parcela experimental se combinaron las unidades básicas para obtener arreglos de diferentes tamaños y formas, a los que se determinó el coeficiente de variación GVij: CVij –Sij/Yij i:1,2, ... L;j = 1, 2, ... A Con el conjunto de los CVij se obtuvo un modelo de regresión cuya expresión real se asume que es un modelo cuadrático en (largo y r (ancho, esto es: CVij= β12+β1α +β2γ+β21αˆ2+β22γˆ2+β12αγ El tamaño óptimo de parcela experimental se estimó en unidades de 6 surcos (a 0,60 m. entre sí de 5 m de longitud (0,10 m entre plantas dentro de surcos, con un coeficiente de variación del 16%. El número de repeticiones estimado, para lograr un coeficiente de variación de 16% y una precisión razonable del 25% es de 4, asumiendo que las pruebas de comparación se hagan con un nivel de significancia de 0,05. A partir del modelo de regresión encontrado, para valores constantes del coeficiente de variación y número de surcos se estimó longitud de los mismos y con todos estos datos se generó una familia de curvas, de tal suerte que para un valor deseado de CV se puede escoger entre varias alternativas de tamaño de

  4. Harina o ensilaje de follajes de leguminosas tropicales como fuente de proteína para cerdos

    OpenAIRE

    Sarria Buenaventura, Patricia Isabel

    2013-01-01

    Bajo la hipótesis “Algunos follajes de leguminosas tropicales ensilados o secos, reemplazan por lo menos el 20% de la proteína de la soya en las dietas balanceadas para cerdos en crecimiento sin afectar el rendimiento productivo” se desarrolló la presente investigación. Dos especies fueron evaluadas: Canavalia brasiliensis CIAT 17009, y Vigna unguiculata CIAT 4555. En experimentos separados para cada leguminosa, la harina de follaje de Canavalia reemplazó hasta el 16% y la harina de Vigna has...

  5. INFLUENCE OF MUNICIPAL SOLID WASTE COMPOST (MSWC ON THE GROWTH AND YIELD OF GREEN GRAM (VIGNA RADIATE (L WILCZEK, FENUGREEK (TRIGONELLA FOENUM-GRAECUM L. AND ON SOIL QUALITY

    Directory of Open Access Journals (Sweden)

    H. Kasthuri

    2011-09-01

    Full Text Available Pot culture experiments were carried out to study the effect of characterized municipal solid waste compost (MSWC amendments (0, 50, 100, 250, 500, 750 and 1000 g with garden soil (6 kg on the growth and the yield of green gram (Vigna radiata (L wilczek and fenugreek (Trigonella foenum-graecum L. as well as changes on soil quality. The growth and the yield of green gram and fenugreek were enhanced by MSWC application upto 500 g. Soil amended with > 500 g MSWC inhibited the plants biometric parameters neither with-significantly nor without-significantly suggest a possible toxic effects to both plants. No dosage effect was detected for total chlorophyll synthesized, except green gram grown in 1000 g of MSWC amendments. Compared with control, percentage of protein was increased significantly upto 250 g for green gram and 500 g for fenugreek. A significant reduction in percentage of carbohydrates was observed above 750 g of MSWC exposure for green gram and above 500 g for fenugreek. There was no specific trend and significant changes were observed in percentage of reducing sugars in both plants except green gram exposed to 50 g MSWC exposure. There was no significant difference in all plant yield parameters of both plants except fenugreek seed weight in 250 g MSWC amendment. Generally, chemical parameters of soil are improved by the addition of MSWC, after 90 d of experimental period.

  6. Molecular cloning and characterization of Vigna mungo processing enzyme 1 (VmPE-1), an asparaginyl endopeptidase possibly involved in post-translational processing of a vacuolar cysteine endopeptidase (SH-EP).

    Science.gov (United States)

    Okamoto, T; Minamikawa, T

    1999-01-01

    Asparaginyl endopeptidase is a cysteine endopeptidase that has strict substrate specificity toward the carboxy side of asparagine residues. Vigna mungo processing enzyme 1, termed VmPE-1, occurs in the cotyledons of germinated seeds of V. mungo, and is possibly involved in the post-translational processing of a vacuolar cysteine endopeptidase, designated SH-EP, which degrades seed storage protein. VmPE-1 also showed a substrate specificity to asparagine residues, and its enzymatic activity was inhibited by NEM but not E-64. In addition, purified VmPE-1 had a potential to process the recombinant SH-EP precursor to its intermediate in vitro. cDNA clones for VmPE-1 and its homologue, named VmPE-1A, were identified and sequenced, and their expressions in the cotyledons of V. mungo seedlings and other organs were investigated. VmPE-1 mRNA and SH-EP mRNA were expressed in germinated seeds at the same stage of germination although the enzymatic activity of VmPE-1 rose prior to that of SH-EP. The level of VmPE-1A mRNA continued increasing as germination proceeded. In roots, stems and leaves of fully grown plants, and in hypocotyls, VmPE-1 and VmPE-1A were little expressed. We discuss possible functions of VmPE-1 and VmPE-1A in the cotyledons of germinated seeds.

  7. Stimulation of dendritic cell maturation and induction of apoptosis in leukemia cells by a heat-stable extract from azuki bean (Vigna angularis), a promising immunopotentiating food and dietary supplement for cancer prevention.

    Science.gov (United States)

    Nakaya, Kazuyasu; Nabata, Yuri; Ichiyanagi, Takashi; An, Wei Wei

    2012-01-01

    Non-toxic stimulation of dendritic cells (DCs), which are central immunomodulators, may aid the prevention of cancer. Furthermore, induction of apoptosis in cancer cells by anticancer agents contributes to the induction of DC maturation. We previously reported that extracts from Pinus parviflora Sieb. et Zucc pine cone and Mucuna seed induce differentiation of mouse bone marrow cells into mature dendritic cells and also induce apoptosis in various human cancer cell lines. In the present study, we screened 31 kinds of edible beans with biological activity similar to that of extracts from pine cone and Mucuna and found that the heat-stable extract from azuki bean (Vigna angula) stimulated differentiation of bone marrow cells into immature DCs with the greatest efficacy. The level of IL-6 produced by sequential treatment of DCs with azuki extract and lipopolysaccharide was the highest among the examined beans. Azuki extract also inhibited the growth of human leukemia U937 cells, leading to induction of apoptosis. These results suggest that azuki bean and its extract are immunopotentiating foods that can be used as a dietary supplement for cancer prevention and immunotherapy. PMID:22524832

  8. Managing the pools of cellular redox buffers and the control of oxidative stress during the ontogeny of drought-exposed mungbean (Vigna radiata L. – role of sulfur nutrition

    Directory of Open Access Journals (Sweden)

    Naser A. Anjum

    2015-01-01

    Full Text Available Impacts of increasing environmental stresses (such as drought on crop productivity can be sustainably minimized by optimizing mineral nutrients (such as sulfur, S. This study, based on a pot-culture experiment conducted in greenhouse condition, investigates S-mediated influence of drought stress (imposed at pre-flowering, flowering and pod-filling stages on growth, photosynthesis and tolerance of mungbean (Vigna radiata L. plants. Drought stress alone hampered photosynthesis functions, enhanced oxidative stress [measured in terms of H2O2; lipid peroxidation (LPO; electrolyte leakage (EL] and decreased the pools of cellular redox buffers (namely ascorbate (AsA; glutathione (GSH], and the overall plant growth (measured as leaf area and plant dry mass, maximally at flowering stage, followed by pre-flowering and pod-filling stages. Contrarily, S-supplementation to drought-affected plants (particularly at flowering stage improved the growth- and photosynthesis-related parameters considerably. This may be ascribed to S-induced enhancements in the pools of reduced AsA and GSH, which jointly manage the balance between the production and scavenging of H2O2 and stabilize cell membrane by decreasing LPO and EL. It is inferred that alleviation of drought-caused oxidative stress depends largely on the status of AsA and GSH via S-application to drought-stressed V. radiata at an appropriate stage of plant growth, when this nutrient is maximally or efficiently utilized.

  9. IRON UPTAKE BY LEAF MESOPHYLL-CELLS - THE ROLE OF THE PLASMA MEMBRANE-BOUND FERRIC-CHELATE REDUCTASE

    NARCIS (Netherlands)

    BRUGGEMANN, W; MAASKANTEL, K; MOOG, PR

    1993-01-01

    The uptake of Fe-59 from FeCl3, ferric (Fe3+) citrate (FeCitr) and Fe3+-EDTA (FeEDTA) was studied in leaf mesophyll of Vigna unguiculata (L.) Walp. Uptake rates decreased in the order FeCl3>FeCitr much greater than FeEDTA, and uptake depended on an obligatory reduction step of Fe3+ to Fe2+, after wh

  10. Socio-economic, agronomic and molecular analysis of yam and cowpea diversity in the Guinea-Sudan transition zone of Benin

    NARCIS (Netherlands)

    Zannou, A.

    2006-01-01

    Keywords: Cowpea (Vigna unguiculata(L.) Walp.),Seed quality in informal seed systems

    OpenAIRE

    Biemond, P.C.

    2013-01-01

    Keywords:     informal seed systems, seed recycling, seed quality, germination, seed pathology, seed health, seed-borne diseases, mycotoxigenic fungi, Fusarium verticillioides, mycotoxins, Vigna unguiculata, Zea mays, Nigeria.   Seed is a crucial input for agricultural production. Approximately 80% of the smallholder farmers in Africa depend for their seed on the informal seed system, consisting of farmers involved in selection, production and dissemination of seed. The la...

  11. Green Fodder Production and Water Use Efficiency of Some Forage Crops under Hydroponic Conditions

    OpenAIRE

    Al-Karaki, Ghazi N.; Al-Hashimi, M.

    2012-01-01

    The objectives of this study were to evaluate five forage crops (alfalfa (Medicago sativa), barley (Hordeum vulgare), cowpea (Vigna unguiculata), sorghum (Sorghum bicolor), and wheat (Triticum aestivum)) for green fodder production and water use efficiency under hydroponic conditions. The experiment has been conducted under temperature-controlled conditions (24 ± 1°C) and natural window illumination at growth room of Soilless Culture Laboratory, Arabian Gulf University, Manama, Bahrain. The r...

  12. Division S-6-soil and water management and conservation : dynamics of soil physical properties in amazonian agroecosystems inoculated with earthworms

    OpenAIRE

    Alegre, J.C.; Pashanasi, B.; Lavelle, P.

    1996-01-01

    The combined use of earthworm inoculation and organic input is considered an efficient way to improve traditional slash-and-burn agriculture in the humid tropics. This study tests the hypothesis that the resistant macroaggregate structure that results from earthworm activities is likely to promote sustainability by favoring water infiltration and soil aeration. Six successive crops (maize (#Zea mays$ L.) - rice (#Oryza sativa$ L.) - cowpea (#Vigna unguiculata$ (L.) Walp.) - rice - rice - rice...

  13. Comparative study on seed sprouting characteristics and drought tolerance of mung bean、cowpea、red bean%绿豆、豇豆、小豆种子萌发特性及耐旱性比较研究

    Institute of Scientific and Technical Information of China (English)

    王云; 师桂花; 张云华; 王芳; 乔宏伟; 白永贵; 王立岩

    2004-01-01

    绿豆(Vigna radiata(L.)Wilczek)、豇豆(Vigna unguiculata L.)、小豆(Vigna angularis(willd)Ohwi&Ohashi)是我国北方旱作区主栽的粮、菜兼用作物,在适宜的环境条件下萌发生长良好,但由于北方春季普遍干旱,严重影响了三种豆类的生产。本文研究了模拟干旱条件下三种豆类种子吸水萌发特性和耐回干能力,以期为生产提供理论依据。

  14. Efficacy of nanostructured silica as a stored pulse protector against the infestation of bruchid beetle, Callosobruchus maculatus (Coleoptera: Bruchidae)

    Science.gov (United States)

    Arumugam, Ganesh; Velayutham, Veeramani; Shanmugavel, Sakthivelkumar; Sundaram, Janarthanan

    2016-03-01

    The treatment of hydrophobic silica nanoparticles (SNPs) with the pulse seeds of Cajanus cajan, Macrotyloma uniflorum, Vigna mungo, Vigna radiata, Cicer arietinum and Vigna unguiculata against the infestation of stored pulse beetle, Callosobruchus maculatus revealed a significant reduction in oviposition, adult emergence and seed damage potential. There was a complete retardation of growth of this beetle in the treated seeds of C. cajan. SNP-treated seeds of these six varieties of pulses revealed no effect on the growth of seeds as revealed by seed germination, growth rate of root and shoot. Similarly, the soil microflora measured in terms of colony forming units was not affected by silica nanoparticles upon its treatment with pulse seeds. The results of this study thus clearly demonstrated the useful nature of silica nanoparticles as seed protecting agent for the control of C. maculatus.

  15. 我国小豆应用核心种质的生态适应性及评价利用%Adaptability and Variation of an Applied Core Collection of Adzuki Bean (Vigna angularis) in China

    Institute of Scientific and Technical Information of China (English)

    王丽侠; 程须珍; 王素华; 罗高玲; 刘振兴; 蔡庆生

    2013-01-01

    To accelerate the application of the core collection of adzuki bean breeding,we investigated the adaptability of these germplasm under different environments at continuous years.The results showed that all the 166core collections were erect and could be harvested in Nanning,while only 88 of them were erect in Tangshan and 25germplasm even could not mature.The agronomic traits were significantly different between eco-environments,and in Nanning,there were also significant variations from different years.The growth periods of the core collections were significantly shorter in Nanning than in Tangshan,however,the number of pods per plant and 100-seed-weight were much lower.Based on the agronomic traits,8 and 10 germplasms were selected to be used in breeding or production at Nanning and Tangshan,respectively.%为提高我国小豆(Vigna angularis)应用核心种质的育种利用效率,本研究通过2年2点的田间试验,分析了166份小豆核心种质农艺性状的变异,明确了其生态适应性和可利用价值.结果表明,166份核心种质在广西南宁均表现为直立生长,并能正常成熟收获;在河北唐山有88份种质2年均表现直立生长,有25份不能正常开花结荚.所有种质在南宁的生育期显著缩短,而单株荚数、百粒重等均显著小于唐山.综合分析表明不同种质的生态适应性存在较大差异.最终根据农艺性状的综合表现,筛选出适合南宁种植的小豆种质8份,适合唐山种植的小豆种质10份.

  16. La sátira en los Cuentos chinos de Abraham Valdelomar

    Directory of Open Access Journals (Sweden)

    Ricardo Silva-Santisteban Ubilluz

    2014-06-01

    Full Text Available Abraham Valdelomar nació en Ica en 1888 y falleció trágicamente en Ayacucho en 1919, luego de una corta pero deslumbrante carrera literaria en la que acometió todos los géneros literarios. En Valdelomar se ha privilegiado al admirable cuentista que fue con detrimento del poeta, el ensayista y el dramaturgo en una obra vasta y desigual en que se destacan dos tonos nítidamente diferenciados: uno exotista y artificial y otro en que se respira y palpa el ambiente de su aldea natal.

  17. El continuo y creciente interés chino en África

    OpenAIRE

    Rabbia, Noemí S.

    2015-01-01

    Desde hace tiempo en el ámbito académico se debate sobre la importancia que han cobrado las denominadas “potencias emergentes” en la escena internacional principalmente en las zonas periféricas a los centros de poder mundial como América Latina y África. En los últimos años dicha discusión puso mayor interés en el rol jugado por los BRICS en esas áreas del mundo que ocuparon un rol marginal en el orden de post guerra fría. A la par de dicho proceso han surgido dudas acerca si se está ante la ...

  18. Propuesta de estrategia para exportación de Tequila al mercado Chino.

    OpenAIRE

    Tapia Sánchez, Jonathan Israel.

    2016-01-01

    La propuesta estratégica de exportación de tequila, es una herramienta para las empresas que desean incursionar en el mercado asiático. Surge, a partir de una idea realizable y fomentada por el Gobierno Federal, por medio de estrategias plausibles que permitan el envío de mercancías a China de manera exitosa. Esta tesis versa con el propósito de conocer la factibilidad de una empresa pequeña en exportar tequila y ver su rentabilidad a mediano plazo. Por medio de un estudio de caso, se analiza...

  19. Chinese automobile industry: opportunities and business; El sector automovilistico Chino: oportunidades y negocios

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Zhang, Y.

    2010-07-01

    The emergence of Chinese economy and its rapid recovery from global crisis have sustained a high rate of growth in Chinese automobile market. The article analyzes the industrial evolution of this particular market from the perspective of automobile manufacturers. Two concrete proposals have been suggested to explore these business opportunities exposed in the analyzed industry: investment in China to take advantage of market growth and hence gaining global competitive advantages, and being strategic partner of Chinese enterprises in European and global market. Strategic alternatives for both proposals have been discussed with suggestions and conclusions followed. (Author) 11 refs.