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Sample records for chinese sturgeon acipenser

  1. Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (Acipenser sturio Linnaeus skin

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    Weiwei Feng

    2013-01-01

    Full Text Available Background: Sturgeon (Acipenser sturio Linnaeus skin contains high amount of nutrients including unsaturated fatty acids and collagen. A pepsin-assisted extraction procedure was developed and optimized for the extraction of collagen from Chinese sturgeon (Acipenser sturio Linnaeus skins. Objective: To determine the optimum conditions with the maximum yield of the pepsin-soluble collagen (PSC extraction. Materials and Methods: The conditions of the extraction were optimized using response surface methodology. The Box-Behnken design was used to evaluate the effects of the three independent variables (extraction time, enzyme concentration, and solid-liquid ratio on the PSC yield of the sturgeon skin. Results: The optimal conditions were: solid-liquid ratio of 1:11.88, enzyme concentration of 2.42%, and extraction time of 6.45 h. The maximum yield of 86.69% of PSC was obtained under the optimal conditions. This value was not significantly different from the predicted value (87.4% of the RSM (P < 0.05. Conclusion: The results of this study indicated that the production of PSC from sturgeon skin is feasible and beneficial. The patterns of sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns (SDS-PAGE indicated that the sturgeon skin contains type I collagen, which is made of α-chain and β-chain. The infrared spectra of the collagens also indicated that pepsin hydrolysis does not affect the secondary structure of collagen, especially triple-helical structure.

  2. De novo annotation of the immune-enriched transcriptome provides insights into immune system genes of Chinese sturgeon (Acipenser sinensis).

    Science.gov (United States)

    Zhu, Rong; Du, He-Jun; Li, Shun-Yi; Li, Ya-Dong; Ni, Hong; Yu, Xue-Jing; Yang, Yan-Yan; Fan, Yu-Ding; Jiang, Nan; Zeng, Ling-Bing; Wang, Xing-Guo

    2016-08-01

    Chinese sturgeon (Acipenser sinensis), one of the oldest extant actinopterygian fishes with very high evolutionary, economical and conservation interest, is considered to be one of the critically endangered aquatic animals in China. Up to date, the immune system of this species remains largely undetermined with little sequence information publicly available. Herein, the first comprehensive transcriptome of immune tissues for Chinese sturgeon was characterized using Illumina deep sequencing. Over 67 million high-quality reads were generated and de novo assembled into the final set of 91,739 unique sequences. The annotation pipeline revealed that 25,871 unigenes were successfully annotated in the public databases, of which only 2002 had significant match to the existing sequences for the genus Acipenser. Overall 22,827 unigenes were categorized into 52 GO terms, 12,742 were classified into 26 KOG categories, and 4968 were assigned to 339 KEGG pathways. A more detailed annotation search showed the presence of a notable representation of immune-related genes, which suggests that this non-teleost actinopterygian fish harbors the same intermediates as in the well known immune pathways from mammals and teleosts, such as pattern recognition receptor (PRR) signaling pathway, JAK-STAT signaling pathway, complement and coagulation pathway, T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways. Additional genetic marker discovery led to the retrieval of 20,056 simple sequence repeats (SSRs) and 327,140 single nucleotide polymorphisms (SNPs). This immune-enriched transcriptome of Chinese sturgeon represents a rich resource that adds to the currently nascent field of chondrostean fish immunogenetics and furthers the conservation and management of this valuable fish. PMID:27368537

  3. Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17β-estradiol on gene expression.

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    Yue, Huamei; Ye, Huan; Chen, Xihua; Cao, Hong; Li, Chuangju

    2013-12-01

    The Chinese sturgeon, Acipenser sinensis, is a rare and large-sized anadromous bony fish and understanding of its reproductive regulation is a precondition for controlled reproduction. In this study, two gonadotropin-releasing hormone (GnRH) precursor cDNAs, AsGnRH1 (mammalian type) and AsGnRH2 (chicken type 2), were sequenced in A. sinensis. The precursor cDNAs of the AsGnRH1 and AsGnRH2 are 381 and 649 base pairs (bp), encoding signal peptide plus precursors of 92 and 86 amino acids, respectively. Multiple sequence alignment suggests that AsGnRH1 and AsGnRH2 decapeptides are highly conserved among vertebrates. Besides, AsGnRH1 had closer evolutionary relationship with tetrapods, while AsGnRH2 was conservatively grouped with teleosts in the phylogenetic analysis. Tissue distribution analysis shows that AsGnRH2 is exclusively transcribed in the brain, whereas AsGnRH1 exhibits more extensive tissue distribution including brain, liver, spleen and gonad. Furthermore, Chinese sturgeons were subcutaneously implanted with 17β-estradiol (E2) and the effect of E2 on brain GnRH mRNA levels was evaluated by real-time PCR. A significant increase in AsGnRH1 and AsGnRH2 mRNA levels is detected in fish receiving E2 implantation compared to controls after one month (P<0.05). These results indicate that E2 exerts positive feedback effects on the transcription of the two GnRHs in immature Chinese sturgeon. PMID:23994573

  4. Identification and Characterization of GH Receptor and Serum GH-binding Protein in Chinese Sturgeon (Acipenser sinensis)

    Institute of Scientific and Technical Information of China (English)

    Zhi-Yong LIAO; Shang-Quan ZHU

    2004-01-01

    Chinese sturgeon, a kind of cartilage ganoid, has a history of over one billion years and it is called the living fossil of aquatic biology since it keeps some evolutionary trace. Here, we characterized the growth hormone receptor (GHR) and serum growth hormone binding protein (GHBP) of Chinese sturgeon. It was shown that GHR was expressed in various tissues, mainly in hepatic, kidney and intestine tissues. GHR on the hepatic membrane has high and specific affinity for bream GH (brGH) and Scatchard analysis of the binding data showed a single class of high affinity binding site with an association constant Ka of 3.1×109 M-1. A specific band around 94 kD was detected by SDS-PAGE in cross-linking studies of membrane receptors. After incubation of Chinese sturgeon serum with 125I-brGH, a 125I-brGH-GHBP complex was identified by Sephadex G-75, indicating that in the serum exists GHBP specially binding to brGH.

  5. Ultrastructure and morphology of spermatozoa in Chinese sturgeon (Acipenser sinensis Gray 1835) using scanning and transmission elektron microscopy

    Czech Academy of Sciences Publication Activity Database

    Wei, Q.; Li, P.; Pšenička, M.; Hadi Alavi, S.M.; Shen, L.; Liu, J.; Pěknicová, Jana; Linhart, O.

    2007-01-01

    Roč. 67, č. 7 (2007), s. 1269-1278. ISSN 0093-691X R&D Projects: GA ČR GA524/06/0817; GA MŠk LC06073 Grant ostatní: GA MŠk 1P05ME742 Institutional research plan: CEZ:AV0Z50520514 Keywords : Acipenser sinensis * spermatozoon * acrosome Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.911, year: 2007

  6. Microsatellite variation in Russian sturgeon (Acipenser gueldenstaedtii from aquaculture

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    Sergiu Emil Georgescu

    2014-05-01

    Full Text Available Normal 0 false false false MicrosoftInternetExplorer4 Most sturgeon species today are on the brink of extinction because of anthropogenic influences, but in the same time, the economical interest regarding these species was constantly increasing, especially because the great value of the roe. In order to satisfy the increasingly high-demand sturgeon farms began developing in the last decades and are nowadays the main producers of caviar and other sturgeon products. Due their characteristics (high polymorphism, high power of discrimination, codominant Mendelian inheritance, microsatellites prove themselves very usefull in population genetics studies. In our study we analyzed the cross-amplification and the polymorphism of seven microsatellite loci (LS-19, LS-54, LS-57, LS-68, Aox9, Aox23, and Aox45 in Acipenser gueldenstaedtii population from aquaculture. The microsatellite markers were originally designed for the American lake sturgeon, Acipenser fulvescens and Atlantic sturgeon, Acipenser oxyrinchus oxyrinchus. We successfully amplified all microsatellite loci obtaining allele peaks of different sizes which were analyzed by capilary electrophoresis. The number of allele ranged beetwen 4 (Aox23 and 12 (LS-54. This technology has great potential for investigating the genetic diversity of the wild sturgeons’ population and, also, might be extended to aquaculture studies aiming the monitoring of genetic variation in comercial breeding programs. /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;}

  7. A comparative gene index for the white sturgeon Acipenser transmontanus.

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    Lazzari, Barbara; Mariani, Valentina; Malinverni, Roberto; Caprera, Andrea; Giuffra, Elisabetta

    2008-03-01

    Sturgeons are archaic fishes phylogenetically distinct from Teleosts. They represent an important niche for aquaculture, particularly for the production of caviar and high quality fillets, while many natural populations in various world areas are today threatened by extinction. Knowledge of the sturgeon genome is limited, as it is the case of many other species of interest for fishery, aquaculture and conservation. Sequences from non-normalized libraries of skin and spleen of the American sturgeon (A. transmontanus) produced in our laboratories were analysed via a bioinformatic procedure, and compared to similar resources available for three Teleost species. Data collected during the analyses were stored in a database - the Sturgeon database (db) - that can be queried via a web interface. The Sturgeon db contains a total of 16,404 sequences from Acipenser transmontanus, Ictalurus punctatus, Salmo salar and Takifugu rubripes, each specie being represented by expressed sequence tags (ESTs) from skin and spleen. Data contained in the database are the results of a number of analyses that mostly focus on sequence annotation and intra- and inter-species comparison. Putative SNP sites, tandem repeats, and sequences matching known protein patterns and motifs were also identified. The Sturgeon db is by now the only online resource dedicated to the analysis of A. transmontanus EST sequences, and represents a starting point for the investigation of the genome of sturgeons from a physiological perspective; it will be used to identify polymorphic markers to study, for example, fish pathologies or to survey fish disease resistance, and to produce gene expression arrays. Introduction of sequences from other species in the analysis pipeline allowed inter-species comparisons of transcripts distribution in Gene Ontology categories, as well as orthologs identification, despite the high sturgeon phylogenetic distance from other fish species. As a result of the EST analysis procedure

  8. Outbreak of mortality in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeons associated with sturgeon nucleo-cytoplasmatic large DNA virus.

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    Ciulli, S; Volpe, E; Sirri, R; Passalacqua, P L; Cesa Bianchi, F; Serratore, P; Mandrioli, L

    2016-08-15

    Diseased outbreaks with high mortality in farmed sturgeon are a limiting factor to the success of this emerging aquaculture sector in Europe. Thorough investigations of outbreaks can determine the aetiological agents, identify important pathological and epidemiological pathways of infections and pave the way for effective control strategies. A thorough investigation of a mortality outbreak in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeons in Italy, demonstrated the primary involvement of a sturgeon nucleo-cytoplasmic large DNA virus (NCLDV). While, the taxonomy classification of this new virus is still uncertain, its involvement in sturgeon mortality outbreaks in Europe is, for the first time, fully investigated and described. Furthermore, the coinfection of bacteria such as motile Aeromonas spp. and Acinetobacter spp. was reported. Genetic characterisation showed the close relationship between the European sturgeon NCLDV with North American sturgeon NCLDVs. Similarly to the latter, the European sturgeon NCLDV persists in survivors. Furthermore, a systemic distribution of the European sturgeon NCLDV was evident in diseased A. baerii and A. gueldenstaedtii and in recovered A. gueldenstaedtii. These epidemiological and pathological findings will help in the identification of effective control strategies for sturgeon NCLDV infection, which afflicts an important and emerging European aquaculture sector. PMID:27374904

  9. Mitochondrial DNA Length Variation and Heteroplasmy in Populations of White Sturgeon (Acipenser Transmontanus)

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    Brown, J R; Beckenbach, A. T.; Smith, M.J.

    1992-01-01

    Southern blot analysis was used to quantify the extent of mtDNA D-loop length variation in two populations of white sturgeon, Acipenser transmontanus. Over 42% of individuals were heteroplasmic for up to six different mtDNA length variants attributable to varying copy numbers of an 82-bp repeat sequence. Chi-square analyses revealed that the frequencies of length genotypes and the incidence of heteroplasmy were significantly different between Fraser and Columbia River sturgeon populations but...

  10. Effects of Dietary Lipids on Growth and Fatty Acid Composition in Russian Sturgeon (Acipenser gueldenstaedtii) Juveniles

    OpenAIRE

    ŞENER, Erdal; Yildiz, Mustafa; SAVAŞ, Esra

    2005-01-01

    Juvenile Russian sturgeon (Acipenser gueldenstaedtii) fed feeds including fish oil, soybean oil and sunflower oil were researched and the effects of the feeds on the growth performance and fatty acid composition of the fish were studied. In the Sapanca Inland Waters Research and Experiment Department of İstanbul University's Faculty of Fisheries, sturgeon juveniles having an approximate initial weight of 27.23 ± 0.98 g were fed experimental feeds containing different kinds of oil for 63 ...

  11. EFFECT OF CRYOPRESERVATION AND THEOPHYLLINE ON MOTILITY CHARACTERISTICS OF LAKE STURGEON (ACIPENSER FULVESCENS) SPERMATOZOA

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    Computer-assisted motility analysis (CASA) was used to evaluate the effect of cryopreservation and theophylline treatment on sperm motility of lake sturgeon (Acipenser fulvescens).Motility was recorded at 0 and 5 min postactivation.The effect of cryopreservation on sperm acrosin-...

  12. Determination of cutting point of oviduct in minimally invasive surgical technique in Persian sturgeon (Acipenser persicus) and Starry sturgeon (Acipenser stellatus)

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    Milad Kabir; Ali Bani

    2011-01-01

    Similar to other Chondrostean fish, Persian sturgeon (Acipenser persicus Borodin, 1897) andStarry sturgeon (Acipenser stellatus Pallas 1771) have got Gymnovarian ovaries, and structure form oftheir reproduction system is such that eggs cannot readily leave it. In minimally invasive surgicaltechnique, a small incision is made in the wall of oviduct so that the eggs can easily leave it with theminimal manipulation and also the broodstock can survive. The aim of this work is to determine thedist...

  13. Artificial reproduction and larval rearing of captive endangered Atlantic sturgeon Acipenser sturio

    OpenAIRE

    WILLIOT P.; Rouault, T; Pelard, M.; Mercier, D.; Jacobs, L

    2009-01-01

    In the early 1990s a functional ex situ brood stock of the European Atlantic sturgeon Acipenser sturio was initiated in France as the only means of saving the species from extinction. In late spring 2007, partial and complete oogenesis and complete spermatogenesis were recorded in fish which were born in the wild in 1994 and entered the hatchery in 1995. Here, we report on the 2007 artificial reproduction attempts, which represent a breakthrough in terms of conservation of A. sturio, and poss...

  14. Length Variation, Heteroplasmy and Sequence Divergence in the Mitochondrial DNA of Four Species of Sturgeon (Acipenser)

    OpenAIRE

    Brown, J R; Beckenbach, K.; Beckenbach, A. T.; Smith, M.J

    1996-01-01

    The extent of mtDNA length variation and heteroplasmy as well as DNA sequences of the control region and two tRNA genes were determined for four North American sturgeon species: Acipenser transmontanus, A. medirostris, A. fulvescens and A. oxyrhnychus. Across the Continental Divide, a division in the occurrence of length variation and heteroplasmy was observed that was concordant with species biogeography as well as with phylogenies inferred from restriction fragment length polymorphisms (RFL...

  15. Fatty acid composition in tissues of the farmed Siberian sturgeon (Acipenser baerii).

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    Nieminen, Petteri; Westenius, Eini; Halonen, Toivo; Mustonen, Anne-Mari

    2014-09-15

    The fatty acid (FA) compositions of the diet and diverse tissues of the farmed Siberian sturgeon (Acipenser baerii) were analyzed in detail to assess their nutritional quality. Twelve male fish were sampled for muscle, fat, liver, brain, gill, kidney and gonad and the tissue FA measured by gas-liquid chromatography. The FA profile of the diet diverged from the FA signatures of the tissues, where the sturgeons accumulated particular highly-unsaturated FA (HUFA). They were probably derived from the diet but, as previous studies have shown that fish can also have desaturase enzymes, endogenous synthesis of these FA cannot be excluded. The sturgeon muscle tissue contained HUFA in proportions comparable to those of other fish species that are considered good sources of n-3 polyunsaturated FA. The indices of atherogenicity and thrombogenicity were also within the values considered to be health-promoting. PMID:24767029

  16. Life history and status of shortnose sturgeon (Acipenser brevirostrum) in the Potomac River

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    Kieffer, Micah

    2009-01-01

    We collected the first life history information on shortnose sturgeon (Acipenser brevirostrum) in any of the rivers to Chesapeake Bay, the geographic center of the species range. In the Potomac River, two telemetry-tagged adult females used 124 km of river: a saltwater/freshwater reach at river km (rkm) 63-141 was the foraging-wintering concentration area, and one female migrated to spawn at rkm 187 in Washington, DC. The spawning migration explained the life history context of an adult captured 122 years ago in Washington, DC, supporting the idea that a natal population once lived in the river. Repeated homing migrations to foraging and wintering areas suggested the adults were residents, not transient coastal migrants. All habitats that adults need to complete life history are present in the river. The Potomac River shortnose sturgeon offers a rare opportunity to learn about the natural rebuilding of a sturgeon population.

  17. Growth and Survival Rate of Russian Sturgeon (Acipenser gueldenstaedtii) Larvae from Fertilized Eggs to Artificial Feeding

    OpenAIRE

    Memiş, D.; Ercan, E.; Çelikkale, M.S.; Timur, M.; Zarkua, Z.

    2009-01-01

    Fertilized eggs of Russian sturgeon (Acipenser gueldenstaedtii) were brought from the Krasnodar Research Institute of Fisheries in 12.01.2001 to the University of Istanbul, Fisheries Faculty, Sapanca Inland Water Fish Culture Research and Applied Station on January 13 th. 5 kg fertilized eggs were put on 13 plates (size of each plate; 65 x 40 x 15 cm). Fertilized eggs were incubated in waving system (model designed according to modified Yushenko apparatus) at 14-15°C by means of water paddles...

  18. Proteome analysis of Persian sturgeon (Acipenser persicus) ova.

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    Keyvanshokooh, Saeed; Vaziri, Behrouz

    2008-12-01

    The Persian sturgeon ova are a key material both for inevitable artificial propagation and for caviar production. In this study, the proteome profile of Persian sturgeon ova was analyzed using 2-DE and MALDI-TOF/TOF in order to determine its protein composition. Out of 192 spots analyzed with MALDI-TOF/TOF, 107 spots corresponding to 73 different proteins were identified. The identified proteins were classified into 11 groups with regard to their main known function involving cell structure (24.65%), translation and transcription (12.32%), metabolism and energy production (12.32%), protein synthesis (9.60%), membrane protein receptors or cell signaling (8.21%), cell defense (5.47%), transport (5.47%), cell division (8.21%), vitellogenin (2.73%), unclassified (6.84%) and unknown function (4.10%). The results of this study provide a valuable resource for molecular analysis of normal and abnormal conditions affecting female reproduction. Moreover, it may help to better understand factors affecting caviar quality during refrigerated storage. PMID:18054827

  19. Comparison of select hematology and serum chemistry analtyes between wild-caught and aquarium-housed lake sturgeon (Acipenser fulvescens)

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    DiVincenti, Louis, Jr.; Priest, Heather; Walker, Kyle J.; Wyatt, Jeffrey D.; Dittman, Dawn

    2013-01-01

    Hematology and serum chemistry analytes were compared between wild-caught and aquarium-housed lake sturgeon (Acipenser fulvescens) to potentially improve understanding of medical issues in lake sturgeon. Blood samples were taken from 30 lake sturgeon exhibited in 11 institutions in the United States and from 23 experimentally stocked lake sturgeon caught in gill nets in the lower Genesee River in Rochester, New York, USA. For hematology, only segmented neutrophil count was significantly different, with wild-caught fish having a higher number of circulating neutrophils. For clinical chemistry analytes, chloride, uric acid, calcium, phosphate, glucose, aspartate aminotransferase, triglycerides, and creatine kinase were significantly different between the two cohorts. These differences are likely not clinically significant and are attributable to handling stress, variability in environmental parameters, or differences in nutritional status. This is the first report of hematology and serum chemistry values in aquarium-housed lake sturgeon and provides useful reference intervals for clinicians.

  20. Microsatellites variation in sterlet sturgeon, Acipenser ruthenus from the Lower Danube

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    Andreea Dudu

    2013-05-01

    Full Text Available Sturgeons represent an ancient group of fish with an important scientific and commercial value. The economical significance of these species is due to their meat and roes that are considered to be a gastronomic delicacy. Unfortunately, precisely due to their economical value the sturgeons have been overexploited by fishing and poaching and nowadays are facing extinction. Currently, in the Ponto-Caspian region is found the greatest diversity of acipenserid species and the Lower Danube is the last refuge for the sturgeons from the Black Sea. Acipenser ruthenus (sterlet is a fresh water sturgeon species which has undergone a large population decline, but local populations are still surviving in most parts of the rivers draining to Black, Azov and Caspian Seas. In order to have successful conservation programs of this species is essential to evaluate its genetic diversity. Microsatellites represent valuable markers for genetic analyses aiming the assessment of genetic variability of population. In our study we analyzed the cross-amplification and the polymorphism in A. ruthenus population from the Lower Danube of seven microsatellite loci (LS-19, LS-34, LS-54, LS-57, LS-68, Aox23 and Aox45, originally isolated in North-American sturgeon species. Among the seven loci, three (LS57, Aox23 and Aox45 have showed a tetrasomic profile. The most polymorphic loci were LS-57 with 12 alleles in population, followed by Aox23 and Aox45 with 11 alleles and LS-68 with 10 alleles. Four loci (LS-19, LS-34 and LS-54 presented a lower level of polymorphism, only three alleles being identified for the analyzed individuals.

  1. Anatomical, hormonal and histological descriptions of captive Russian sturgeon (Acipenser gueldenstaedtii) with intersex gonads.

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    Jackson, Karen; Hurvitz, Avshalom; Din, Svetlana Yom; Goldberg, Doron; Pearlson, Oren; Degani, Gad; Levavi-Sivan, Berta

    2006-09-15

    Sturgeons are known throughout the world as the source of black caviar. Their declining populations in their native habitats, mainly the Caspian Sea, due to over-fishing for meat and caviar production, destruction of their spawning grounds and water pollution, have led to their introduction into aquaculture in areas with suitable conditions, including Israel. Recently, we noticed an unusual phenomenon in these normally gonochoristic species. Several 5-year-old female sturgeons were found to have one or more testicular sections in each of their two gonads, forming an intersexual gender. Further examination of other fish from the same age group revealed 14% fish with intersex gonads among a population of 5000 fish that had been pre-selected as females. This phenomenon has not been found however in other age groups of Russian sturgeons, cultured at the same facility. Sturgeons are a generally gonochoristic species, and hermaphroditism is only very infrequently observed under natural or normal breeding conditions. Moreover, these rare cases have all been from polluted habitats. The present work is the first description of fish containing intersex gonads in Russian sturgeon (Acipenser gueldenstaedtii). We describe the phenomenon anatomically and histologically, and examine plasma steroid levels and pituitary gonadotropin gene expression by comparing fish with intersex gonads with normal females and males of the same age group. Intersex gonads were typical female ovaries with one or more white testicular components embedded in each. The testis components were not uniform in size or location among the two gonads of each fish or among different fish, and they showed marked differences in distribution. The ovarian component of the intersex gonad was at the pre-vitellogenic stage as in normal females, and the testis component contained spermatids and mature spermatozoa as in normal males of the same age. However, in terms of estradiol and 11-ketotestosterone plasma levels

  2. Transcriptome analysis and de novo annotation of the critically endangered Amur sturgeon (Acipenser schrenckii).

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    Zhang, X J; Jiang, H Y; Li, L M; Yuan, L H; Chen, J P

    2016-01-01

    The aim of this study was to provide comprehensive insights into the genetic background of sturgeon by transcriptome study. We performed a de novo assembly of the Amur sturgeon Acipenser schrenckii transcriptome using Illumina Hiseq 2000 sequencing. A total of 148,817 non-redundant unigenes with base length of approximately 121,698,536 bp and ranges from 201 to 26,789 bp were obtained. All the unigenes were classified into 3368 distinct categories and 145,449 singletons by homologous transcript cluster analysis. In all, 46,865 (31.49%) unigenes showed homologous matches with Nr database and 32,214 (21.65%) unigenes were matched to Nt database. In total, 24,862 unigenes were categorized into significantly enriched 52 function groups by GO analysis, and 38,436 unigenes were classified into 25 groups by KOG prediction, as well as 128 enriched KEGG pathways were identified by 45,598 unigenes (P < 0.05). Subsequently, a total of 19,860 SSRs markers were identified with the abundant di-nucleotide type (10,658; 53.67%) and the most AT/TA motif repeats (2689; 13.54%). A total of 1341 conserved lncRNAs were identified by a customized pipeline. Our study provides new sequence and function information for A. schrenckii, which will be the basis for further genetic studies on sturgeon species. The huge number of potential SSRs and putatively conserved lncRNAs isolated by the transcriptome also shed light on research in many fields, including the evolution, conservation management, and biological processes in sturgeon. PMID:27420941

  3. Selected heavy metals analysis of Persian sturgeon's (Acipenser persicus) caviar from Southern Caspian Sea.

    Science.gov (United States)

    Hosseini, S V; Sobhanardakani, S; Tahergorabi, R; Delfieh, P

    2013-09-01

    The present research reports the heavy metal (Fe, Cr, Pb, As, and Co) contamination in caviar of Persian sturgeon (Acipenser persicus) caught from Southern Caspian Sea sampling site using ICP-OES. Heavy metal concentrations (microgram per gram, wet weight; means ± S.D.) in caviar samples were: Fe, 71.33 ± 0. 37; Cr, 0.27 ± 0.019; Pb, <0.01 ± 0.002; As, <0.01 ± 0.002; and Co, <0.01 ± 0.001, respectively. Comparative evaluation of these metals in different samples showed that except Fe, the average concentrations of Cr, Pb, As, and Co are significantly lower than adverse level for the species themselves and for human consumption when compared with FAO/WHO permissible limits. Therefore, their contribution to adverse health effects on human body can be considered as negligibly small. PMID:23824563

  4. Penicillin improves the milt quality of Persian sturgeon, Acipenser persicus during short-term storage

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    Mostafa Halimi

    2014-04-01

    Full Text Available This study was conducted to examine the effects of antibiotic (5000 units of penicillin on sperm quality of Persian sturgeon, Acipenser persicus during 9 days in vitro storage of milt. For this purpose, the milt samples were stored in the presence and absence of 5000 units of penicillin. Freshwater was used as sperm activator. The milt samples were stored at 4°C and the motility indices were measured 0, 3, 6 and 9 days after storage. The sperm duration and percentage of sperm motility decreased after 6 days of storage both in the presence and absence of antibiotic, although this decrease was more significant in the absence of antibiotic. After 9 days of storage, the lowest values of sperm motility indices was recorded for antibiotic receiving milt samples while no motile spermatozoa observed for antibiotic-free milt samples. In conclusion, our results demonstrated that 5000 units of penicillin improve the Persian sturgeon milt quality during short-term storage.

  5. Research regarding bioproductive indicators achieved by the Siberian sturgeon (Acipenser baerii juvenile in recirculated system

    Directory of Open Access Journals (Sweden)

    Zoltan T. Szelei

    2011-10-01

    Full Text Available For this research we used a population of 2799 Siberian sturgeons (Acipenser baerii between11.04.2009 and 23.05.2009. The age of juveniles was between 316 days and 360 days and they weremaintained in 4 tanks with a volume of 12.56 m3/tank. Average water temperature in the breeding tankswas 17.3 0C in the first study period, 18 0C in the second period and 19.8 0C in the third period. In thisperiod the average value of dissolved oxygen level in the tanks was 5.1 mg/L. The Siberian sturgeonjuveniles were fed with Agua Bio Sturion (Ø=3 mm; 45% Protein and 20% Fat. At every 2 weeks, theaverage body weight and the average body length of 30 individuals from each tank were registered. At theage of 316 days the juveniles reached an average body weight between 164.43±7.63 g and 325.80±15.20g and an average body length between 35.78±0.48 cm and 45.31±0.66 cm. During the 44 experimentaldays, the 2799 Siberian sturgeons have achieved an real weight gain of 310.67 kg, with a specificconsumption of 0.67 kg feed/kg body weight gain and an index of feed conversion of 1.48 kg body weightgain/kg of consumed feed.

  6. Habitat used by juvenile lake sturgeon (Acipenser fulvescens) in the North Channel of the St. Clair River (Michigan, USA)

    Science.gov (United States)

    Boase, James C.; Manny, Bruce A.; Donald, Katherine A.L.; Kennedy, Gregory W.; Diana, James S.; Thomas, Michael V.; Chiotti, Justin A.

    2014-01-01

    Lake sturgeon (Acipenser fulvescens) occupy the St. Clair River, part of a channel connecting lakes Huron and Erie in the Laurentian Great Lakes. In the North Channel of the St. Clair River, juvenile lake sturgeon (3–7 years old and 582–793 mm in length) were studied to determine movement patterns and habitat usage. Fourteen juveniles were implanted with ultrasonic transmitters and tracked June–August of 2004, 2005 and 2006. Telemetry data, Geographic Information System software, side-scan sonar, video images of the river bottom, scuba diving, and benthic substrate samples were used to determine the extent and composition of habitats they occupied. Juvenile lake sturgeon habitat selection was strongly related to water depth. No fish were found in 700 mm in length selected sand and gravel areas mixed with zebra mussels and areas dominated by zebra mussels, while fish < 700 mm used these habitat types in proportion to their availability.

  7. Stress and its relation to endocrine function in captive female Siberian sturgeon (Acipenser baeri).

    Science.gov (United States)

    Hamlin, Heather J; Edwards, Thea M; Moore, Brandon C; Main, Kevan L; Guillette, Louis J

    2007-01-01

    Stress responses to numerous environmental conditions have been studied in a wide range of fish species. Defining the relationship between stress and endocrine function is particularly critical to long-lived species such as sturgeons, whose economic viability relies heavily on proper endocrine function for the production of caviar. In this study, we examined the induced stress response, defined by plasma cortisol and glucose concentrations, and its relationship to plasma 17beta-estradiol, testosterone and 11-ketotestosterone concentrations in cultured female Siberian sturgeon (Acipenser baeri). Fish were acutely stressed using capture and confinement for a period of 4 h, and blood samples were drawn according to four treatment regimens: treatment 1, fish were bled at 0 h; treatment 2, fish were serially bled at 0 h, 1 h and 4 h; treatment 3, fish were bled at 1 h and 4 h; treatment 4, fish were bled at 4 h only. Fish were surgically sexed immediately after drawing blood at 0 h. After 1 h of acute stress, fish demonstrated a marked increase in plasma cortisol concentration, which remained elevated throughout the 4-h sampling period. The plasma concentration of sex steroids did not exhibit an inverse relationship with plasma cortisol concentration. Plasma testosterone concentration was significantly elevated during the periods of greatest stress. Plasma estradiol and 11-ketotestosterone (11-KT) concentrations were not affected by the acute stressors in this experiment. Serial bleedings, however, affected the associated stress response, which is an important consideration for future studies with this species. This is the first study to define the relationship between stress and possible changes in sex steroid concentration in this threatened and commercially important species. PMID:17622217

  8. Electronic archival tags provide first glimpse of bathythermal habitat use by free-ranging adult lake sturgeon Acipenser fulvescens

    Science.gov (United States)

    Briggs, Andrew S.; Hondorp, Darryl W.; Quinlan, Henry R.; Boase, James C.; Mohr, Lloyd C.

    2016-01-01

    Information on lake sturgeon (Acipenser fulvescens) depth and thermal habitat use during non-spawning periods is unavailable due to the difficulty of observing lake sturgeon away from shallow water spawning sites. In 2002 and 2003, lake sturgeon captured in commercial trap nets near Sarnia, Ontario were implanted with archival tags and released back into southern Lake Huron. Five of the 40 tagged individuals were recaptured and were at large for 32, 57, 286, 301, and 880 days. Temperatures and depths recorded by archival tags ranged from 0 to 23.5 ºC and 0.1 to 42.4 m, respectively. For the three lake sturgeon that were at large for over 200 days, temperatures occupied emulated seasonal fluctuations. Two of these fish occupied deeper waters during winter than summer while the other occupied similar depths during non-spawning periods. This study provides important insight into depth and thermal habitat use of lake sturgeon throughout the calendar year along with exploring the feasibility of using archival tags to obtain important physical habitat attributes during non-spawning periods.

  9. Quality attributes and microbial storage stability of caviar from cultivated white sturgeon (Acipenser transmontanus).

    Science.gov (United States)

    Shin, Joong-Han; Oliveira, Alexandra C M; Rasco, Barbara A

    2010-01-01

    Caviar was prepared from white sturgeon (Acipenser transmontanus) roe by adjusting the water phase salt (WPS) to 4.0% to 6.3% by adding food grade NaCl. Fish were obtained from 2 different farms from the Inland Northwest (N = 5). Salt was absorbed at a different rate and to a different extent by roe from different fish. The lipid content in the fish roe varied from 10.2% to 14.4% (w/w), with palmitic acid and oleic acid being the most abundant saturated and monounsaturated fatty acids present, respectively. The caviar contained high levels of polyunsaturated fatty acids (PUFA) (35% to 37%) with docosahexanoic acid being the most abundant omega-3 long chain fatty acid. There were no significant differences in microbial storage stability for caviars from different fish stored at 3 degrees C. However, for caviar stored at 7 degrees C, there was less growth of Listeria monocytogenes (using a cocktail of ATCC 19114, 7644, 19113 strains) in 2 samples (2B46 and 0F05) until day 20. In 2 other samples (453F and 2519), which had lower initial microbial loads, less overall microbial growth was observed, indicating that culture and harvest practices result in compositional differences between fish, which may impact both product composition and storage stability. PMID:20492148

  10. Fatty acid composition and volatile compounds of caviar from farmed white sturgeon (Acipenser transmontanus).

    Science.gov (United States)

    Caprino, Fabio; Moretti, Vittorio Maria; Bellagamba, Federica; Turchini, Giovanni Mario; Busetto, Maria Letizia; Giani, Ivan; Paleari, Maria Antonietta; Pazzaglia, Mario

    2008-06-01

    The present study was conducted to characterize caviar obtained from farmed white sturgeons (Acipenser transmontanus) subjected to different dietary treatments. Twenty caviar samples from fish fed two experimental diets containing different dietary lipid sources have been analysed for chemical composition, fatty acids and flavour volatile compounds. Fatty acid make up of caviar was only minimally influenced by dietary fatty acid composition. Irrespective of dietary treatments, palmitic acid (16:0) and oleic acid (OA, 18:1 n-9) were the most abundant fatty acid followed by docosahexaenoic acid (DHA, 22:6 n-3) and eicopentaenoic (EPA, 20:5 n-3). Thirty-three volatile compounds were isolated using simultaneous distillation-extraction (SDE) and identified by GC-MS. The largest group of volatiles were represented by aldehydes with 20 compounds, representing the 60% of the total volatiles. n-Alkanals, 2-alkenals and 2,4-alkadienals are largely the main responsible for a wide range of flavours in caviar from farmed white surgeon. PMID:18486649

  11. Optimizing the co-feeding strategy of Persian sturgeon (Acipenser persicus larvae using Artemia nauplii and formulated diet

    Directory of Open Access Journals (Sweden)

    Naser Agh

    2013-07-01

    Full Text Available High mortality and labor costs are associated with first-feeding sturgeon culture, particularly during the period of dietary transition from live to formulated feed. Therefore we investigated the effects of various feeding treatments on the survival and growth of the Persian sturgeon (Acipenser persicus larvae during a 20-day culture period. Three replicate groups (250 fish/replicate of first-feeding larvae were fed according to four main feeding regimes: (1 live food (live nauplii of brine shrimp Artemia urmiana; (2 indirect transition (5 days live food followed by gradual transition to formulated diet; (3 direct transition (using different combinations of live and formulated diet from the start feeding onwards; (4 formulated feed (FD from the start feeding. Results indicated that growth and survival were higher in the indirect transition feeding regime than in other regimes. Based on our study, co-feeding of A. persicus should start five days after prior feeding with live food.

  12. Competition between selenomethionine and methionine absorption in the intestinal tract of green sturgeon (Acipenser medirostris)

    International Nuclear Information System (INIS)

    L-Selenomethionine (SeMet) is a dominant form of selenium (Se) found in organisms at all levels of aquatic food chains and a key source of Se bioaccumulation and ecotoxicity. In mammals, intestinal absorption of SeMet is at least partly via the Na+-dependent neutral amino acid transporter. The mechanism of SeMet absorption and competitive effects of other dietary components on SeMet absorption in fish are unknown. Thus the in vitro uptake rates of L-methionine (Met) and the competitive effect of SeMet on Met absorption, an indicator that SeMet uses the same nutrient transporter(s) for absorption, in the various regions of the green sturgeon (Acipenser medirostris) intestine were investigated using intact tissues (a modified everted sleeve method). Intestinal tissue was incubated in Ringer's solution containing 0-10 mmol L-1 Met or SeMet (n = 5 for each substrate's concentration and intestinal region), respectively, as well as constant tracer levels of isotope-labeled Met. The data indicate that SeMet uptake was mediated by the same transporter(s) as Met and that the absorption kinetics were similar for both substrates. When there were differences in absorption they appeared to be mostly due to higher permeability (passive uptake) of the tissue for Met than for SeMet, particularly in the pyloric caeca (PC) and distal intestine (DI). Maximum rates of absorption, on the other hand, tended to be higher for SeMet than Met in the mid intestine and DI, whereas differences in affinity for the transporters varied between these tissues but were very similar in the PC. These differences may be due to differences in regional intestinal characteristics such as amount of mucus secreted and degree of tissue contraction, and/or substrate differences regarding solubility in and movement through the mucus, influence on tissue contraction, permeability through membranes or between cells, intracellular metabolism, as well as basolateral transport. Interestingly, an increasing proximal

  13. Ancient fish and recent invaders: white sturgeon Acipenser transmontanus diet response to invasive-species-mediated changes in a benthic prey assemblage

    Science.gov (United States)

    Zeug, Steven C; Brodsky, Annie; Kogut, Nina; Stewart, Robin; Merz, Joe

    2014-01-01

    Invasive organisms can have significant impacts on native species, and the San Francisco Estuary (SFE), California, USA, is one of the world's most invaded estuaries. Decline of native white sturgeon Acipenser transmontanus abundance in the SFE has been acknowledged, but underlying mechanisms are poorly understood. Invasion by the overbite clam Potamocorbula amurensis has drastically altered the SFE benthic prey community, yet little is known about how this change has affected sturgeon diets. We investigated changes in the diet of white sturgeon following the overbite clam invasion and subsequent shift in the SFE benthic prey assemblage. Gut content analysis was used to compare white sturgeon prey composition and importance between the pre- and post-invasion periods. Additionally, stable isotope analysis was employed to estimate the assimilation of prey items to sturgeon biomass. Overbite clams dominated diets in the post-invasion period, accounting for 82 to 93% of total volume. Stable isotope analysis confirmed the importance of this prey item, although their assimilated contribution to sturgeon biomass was estimated to be less (70 to 83%) than gut contents indicated. The frequency of fish in white sturgeon guts increased in the post-invasion period, and isotope analysis indicated relatively large contributions of fish to sturgeon biomass (3.7 to 19%). The trophic adaptability of white sturgeon has allowed them to exploit this new prey source (overbite clam). Future conservation and restoration efforts must consider a potentially destabilized food web given the large importance of a single prey item.

  14. 78 FR 58507 - Endangered and Threatened Wildlife and Plants; 90-Day Finding on a Petition To List 10 Sturgeon...

    Science.gov (United States)

    2013-09-24

    ... construction, and water pollution. The petition is available at http://www.nmfs.noaa.gov/pr/pdfs/petitions... sturgeon (Acipenser baerii). Endangered Decreasing China; Kazakhstan; Mongolia; Russia. Yangtze sturgeon (Acipenser Critically Endangered.. Decreasing China. dabryanus). Russian sturgeon (Acipenser...

  15. Effects of Lampricide on Olfaction and Behavior in Young-of-the-Year Lake Sturgeon (Acipenser fulvescens).

    Science.gov (United States)

    Sakamoto, Kathrine; Dew, William A; Hecnar, Stephen J; Pyle, Gregory G

    2016-04-01

    The lampricide, 3-trifluoromethyl-4-nitrophenol (TFM), is a primary component to sea lamprey control in the Laurentian Great Lakes. Though the lethal effects of TFM are well-known, the sublethal effects on fishes are virtually unknown. Here we studied the effects of TFM on the olfactory capabilities and behavior of young-of-the-year (YOY) lake sturgeon (Acipenser fulvescens). At ecologically relevant concentrations of TFM there was reduced olfactory response to all three cues (l-alanine, taurocholic acid, food cue) tested, suggesting that TFM inhibits both olfactory sensory neurons tested. Sturgeon exposed to TFM also showed a reduced attraction to the scent of food and reduced consumption of food relative to unexposed fish. Exposed fish were more active than control fish, but with slower acceleration. Fish were able to detect the scent of TFM, but failed to avoid it in behavioral trials. The connection between neurophysiological and behavioral changes, and the commonality of habitats between sturgeon and lamprey ammocoetes, suggests that there may be effects at the ecosystem level in streams that undergo lamprey control treatments. PMID:27015540

  16. HYDRODYNAMIC CHARACTERISTICS OF CHINESE STURGEON SPAWNING GROUND IN YANGTZE RIVER

    Institute of Scientific and Technical Information of China (English)

    YANG Yu; YAN Zhong-min; CHANG Jian-bo

    2008-01-01

    Since the construction of the Gezhouba Dam on the Yangtze River in 1981, it has been found that the Chinese Sturgeon, Acipensor sinensis, performs natural propagation annually in a narrow reach downstream close to the Gezhouba Dam site. This might allow one to better investigate the river hydrodynamic conditions of the Chinese Sturgeon spawning ground. In this article, spatial Kinetic Energy Gradient (KEG) and absolute value of vorticity were computed along the river sections from measured data. The relation between Spawn Density per Unit Area (SDUA) and vorticity strength and between SDUA and KEG were worked out. The Results showed that the vorticity and KEG were both effective parameters for describing the hydrodynamic characteristics of Chinese Sturgeon spawning ground. The Chinese Sturgeons prefer to spawn in the river sections where the value of vorticity strength is larger than 0.4 s-1 and KEG is larger than 0.029 Jkg-1m-1.

  17. Application of molecular and cytogenetic markers in sturgeon aquaculture: a case on sterlet (Acipenser ruthenus) and Russian sturgeon (Acipenser gueldenstaedtii) with odd ploidy levels

    Czech Academy of Sciences Publication Activity Database

    Havelka, M.; Hulák, M.; Bytyutskyy, D.; Rábová, Marie; Ráb, Petr; Rodina, M.; Gela, D.; Flajšhans, M.

    Alabama : AUBURN University, 2012. s. 65-65. [International Symposium on Genetics in Aquaculture /11./. 25.06.2012-29.06.2012, Alabama] R&D Projects: GA ČR GA523/08/0824 Institutional support: RVO:67985904 Keywords : sturgeon aquaculture Subject RIV: EB - Genetics ; Molecular Biology

  18. Lateralisation of rotational swimming but not fast escape response in the juvenile sterlet sturgeon, Acipenser ruthenus (Chondrostei: Acipenseridae).

    Science.gov (United States)

    Izvekov, Evgeny I; Kuternitskaya, Elena A; Pankova, Natalya A; Malashichev, Yegor B; Nepomnyashchikh, Valentin A

    2014-01-01

    For the first time, behavioural lateralisation was shown in a chondrostean fish (sterlet sturgeon Acipenser ruthenus). A significant directional bias was found in young A. ruthenus swimming along a circular swimway. This laterality manifested itself as an individual preference for a certain movement direction (either clockwise or counterclockwise) which was consistent at the retest 10 days later. On the other hand, no significant rotational bias was observed at the population level. The same sterlet individuals displayed the C-start (the first stage of escape response) elicited by sudden low-frequency sound vibrations (50 Hz). However, the experiments failed to reveal either individual or population laterality of this response: the frequencies of leftward and rightward bends in startled fish were virtually equal. These results demonstrate that the two types of laterality can be independent in fish. PMID:23758344

  19. Comparative transcriptome analysis of testes and ovaries for the discovery of novel genes from Amur sturgeon (Acipenser schrenckii).

    Science.gov (United States)

    Jin, S B; Zhang, Y; Dong, X L; Xi, Q K; Song, D; Fu, H T; Sun, D J

    2015-01-01

    Sturgeons (Acipenser schrenckii) are of high evolutionary, economic, and conservation value, and caviar isone of the most valuable animal food products in the world. The Illumina HiSeq2000 sequencing platform was used to construct testicular and ovarian transcriptomes to identify genes involved in reproduction and sex determination in A. schrenckii. A total of 122,381 and 114,527 unigenes were obtained in the testicular and ovarian transcriptomes, respectively, with average lengths of 748 and 697 bp. A total of 46,179 genes were matched to the non-redundant nr database. GO (31,266), KEGG (39,712), and COG analyses (20,126) were performed to identify potential genes and their functions. Twenty-six gene families involved in reproduction and sex determination were identified from the A. schrenckii testicular and ovarian transcriptomes based on functional annotation of non-redundant transcripts and comparisons with the published literature. Furthermore, 1309 unigenes showed significant differences between the testes and ovaries, including 782 genes that were up-regulated in the testes and 527 that were up-regulated in the ovaries. Eleven genes were involved in reproduction and sex determination mechanisms. Furthermore, 19,065 simple sequence repeats (SSRs) were identified in the expressed sequence tagged dataset, and 190,863 and 193,258 single nucleotide polymorphisms (SNPs) were obtained from the testicular and ovarian transcriptomic databases, respectively. This study provides new sequence information about A. schrenckii, which will provide a basis for the further study of reproduction and sex determination mechanisms in Acipenser species. The potential SSR and SNP markers isolated from the transcriptome may shed light on the evolution and molecular ecology of Acipenser species. PMID:26782541

  20. Destruction of Listeria monocytogenes in sturgeon (Acipenser transmontanus) caviar by a combination of nisin with chemical antimicrobials or moderate heat.

    Science.gov (United States)

    Al-Holy, M; Lin, M; Rasco, B

    2005-03-01

    The objective of this study was to investigate the effect of nisin in combination with heat or antimicrobial chemical treatments (such as lactic acid, chlorous acid, and sodium hypochlorite) on the inhibition of Listeria monocytogenes and total mesophiles in sturgeon (Acipenser transmontanus) caviar. The effects of nisin (250, 500, 750, and 1,000 IU/ml), lactic acid (1, 2, and 3%), chlorous acid (134 and 268 ppm), sodium hypochlorite (150 and 300 ppm), and heat at 60 degrees C for 3 min were evaluated for a five-strain mixture of L. monocytogenes and total mesophiles in sturgeon caviar containing 3.5% salt. Selected combinations of these antimicrobial treatments were also tested. Injured and viable L. monocytogenes cells were recovered using an overlay method. Treating caviar with > or =500 IU/ml nisin initially reduced L. monocytogenes by 2 to 2.5 log units. Chlorous acid (268 ppm) reduced L. monocytogenes from 7.7 log units to undetectable (caviar treated with heat and nisin (750 IU/ml) after a storage period of 28 days at 4 degrees C. PMID:15771175

  1. Inactivation of Listeria innocua in nisin-treated salmon (Oncorhynchus keta) and sturgeon (Acipenser transmontanus) caviar heated by radio frequency.

    Science.gov (United States)

    Al-Holy, M; Ruiter, J; Lin, M; Kang, D H; Rasco, B

    2004-09-01

    Recent regulatory concerns about the presence of the pathogen Listeria monocytogenes in ready-to-eat aquatic foods such as caviar has prompted the development of postpackaging pasteurization processes. However, caviar is heat labile, and conventional pasteurization processes affect the texture, color, and flavor of these foods negatively. In this study, chum salmon (Oncorhynchus keta, 2.5% total salt) caviar or ikura and sturgeon (Acipenser transmontanus, 3.5% total salt) caviar were inoculated with three strains of Listeria innocua in stationary phase at a level of more than 10(7) CFU/g. L innocua strains were used because they exhibit an equivalent response to L monocytogenes for many physicochemical processing treatments, including heat treatment. The products were treated by immersion in 500 IU/ml nisin solution and heat processed (an 8-D process without nisin or a 4-D process with 500 IU/ml nisin) in a newly developed radio frequency (RF; 27 MHz) heating method at 60, 63, and 65 degrees C. RF heating along with nisin acted synergistically to inactivate L. innocua cells and total mesophilic microorganisms. In the RF-nisin treatment at 65 degrees C, no surviving L. innocua microbes were recovered in sturgeon caviar or ikura. The come-up times in the RF-heated product were significantly lower compared with the water bath-heated caviar at all treatment temperatures. The visual quality of the caviar products treated by RF with or without nisin was comparable to the untreated control. PMID:15453574

  2. Dietary Administration of Lactobacillus plantarum Enhanced Growth Performance and Innate Immune Response of Siberian Sturgeon, Acipenser baerii.

    Science.gov (United States)

    Pourgholam, Moheb Ali; Khara, Hossein; Safari, Reza; Sadati, Mohammad Ali Yazdani; Aramli, Mohammad Sadegh

    2016-03-01

    We investigated the effects of Lactobacillus plantarum used as a dietary supplement on the growth performance and innate immune response in juvenile Siberian sturgeon Acipenser baerii. Juvenile fish (14.6 ± 2.3 g) were fed three experimental diets prepared by supplementing a basal diet with L. plantarum at different concentrations [1 × 10(7), 1 × 10(8) and 1 × 10(9) colony-forming units (cfu) g(-1)] and a control (non-supplemented basal) diet for 8 weeks. Growth performance indices were increased in fish fed the 1 × 10(8) cfu g(-1) L. plantarum diet compared to the other groups. There was an increased innate immune response in fish fed the experimental diets. The highest levels of lysozyme activity, total immunoglobulin (IgM) and complement component 3 (C3) were observed in fish fed the diet containing L. plantarum at a concentration of 1 × 10(8) cfu g(-1), but there was no significant difference in the level of complement component 4 (C4) in fish fed the experimental diets or the control diet. The present study underlying some positive effects (growth performance and immune indices) of dietary administration of L. plantarum at a concentration of 1 × 10(8) cfu g(-1) in the Siberian sturgeon. PMID:26686864

  3. Seasonal variation in plasma nonesterified fatty acids of Lake Sturgeon (Acipenser fulvescens) in the vicinity of hydroelectric facilities

    International Nuclear Information System (INIS)

    To establish the effects of hydroelectric generation on the health of lake sturgeon (Acipenser fulvescens), seasonal variations in plasma nonesterified fatty acids (NEFAs) upstream and downstream from hydroelectric stations were measured over a 2-y period. Plasma NEFA profiles were also compared up- and downstream of the stations for differences in utilization of individual NEFA species as substrates for lipid oxidation. Significantly higher levels of total plasma NEFA were found in lake sturgeon upstream (2355 ± 395.9 nmole/ml) compared with those downstream (798 ± 133.5 nmole/ml) of the generating stations during the spring. The NEFA profiles for several key fatty acid species differed significantly among seasons up- and downstream of the facilities. In particular, during spring and summer, the levels of oleic acid (18:1n9) were highest upstream of the stations and levels of a polyunsaturated fatty acid, docosahexaenoic acid (22:6n3), were higher below rather than above the stations. The differences in plasma NEFA concentration may be attributed to altered nutritional status due to the varying flow regime located downstream of the hydroelectric stations. 33 refs., 4 figs., 1 tab

  4. A nuclear DNA perspective on delineating evolutionarily significant lineages in polyploids: the case of the endangered shortnose sturgeon (Acipenser brevirostrum)

    Science.gov (United States)

    King, Timothy L.; Henderson, Anne P.; Kynard, Boyd E.; Kieffer, Micah C.; Peterson, Douglas L.; Aunins, Aaron W.; Brown, Bonnie L.

    2014-01-01

    The shortnose sturgeon, Acipenser brevirostrum, oft considered a phylogenetic relic, is listed as an “endangered species threatened with extinction” in the US and “Vulnerable” on the IUCN Red List. Effective conservation of A. brevirostrum depends on understanding its diversity and evolutionary processes, yet challenges associated with the polyploid nature of its nuclear genome have heretofore limited population genetic analysis to maternally inherited haploid characters. We developed a suite of polysomic microsatellite DNA markers and characterized a sample of 561 shortnose sturgeon collected from major extant populations along the North American Atlantic coast. The 181 alleles observed at 11 loci were scored as binary loci and the data were subjected to multivariate ordination, Bayesian clustering, hierarchical partitioning of variance, and among-population distance metric tests. The methods uncovered moderately high levels of gene diversity suggesting population structuring across and within three metapopulations (Northeast, Mid-Atlantic, and Southeast) that encompass seven demographically discrete and evolutionarily distinct lineages. The predicted groups are consistent with previously described behavioral patterns, especially dispersal and migration, supporting the interpretation that A. brevirostrum exhibit adaptive differences based on watershed. Combined with results of prior genetic (mitochondrial DNA) and behavioral studies, the current work suggests that dispersal is an important factor in maintaining genetic diversity in A. brevirostrum and that the basic unit for conservation management is arguably the local population.

  5. Managing polyploidy in ex situ conservation genetics: the case of the critically endangered Adriatic sturgeon (Acipenser naccarii).

    Science.gov (United States)

    Congiu, Leonardo; Pujolar, Jose Martin; Forlani, Anna; Cenadelli, Silvia; Dupanloup, Isabelle; Barbisan, Federica; Galli, Andrea; Fontana, Francesco

    2011-01-01

    While the current expansion of conservation genetics enables to address more efficiently the management of threatened species, alternative methods for genetic relatedness data analysis in polyploid species are necessary. Within this framework, we present a standardized and simple protocol specifically designed for polyploid species that can facilitate management of genetic diversity, as exemplified by the ex situ conservation program for the tetraploid Adriatic sturgeon Acipenser naccarii. A critically endangered endemic species of the Adriatic Sea tributaries, its persistence is strictly linked to the ex situ conservation of a single captive broodstock currently decimated to about 25 individuals, which represents the last remaining population of Adriatic sturgeon of certain wild origin. The genetic variability of three F1 broodstocks available as future breeders was estimated based on mitochondrial and microsatellite information and compared with the variability of the parental generation. Genetic data showed that the F1 stocks have only retained part of the genetic variation present in the original stock due to the few parent pairs used as founders. This prompts for the urgent improvement of the current F1 stocks by incorporating new founders that better represent the genetic diversity available. Following parental allocation based on band sharing values, we set up a user-friendly tool for selection of candidate breeders according to relatedness between all possible parent-pairs that secures the use of non-related individuals. The approach developed here could also be applied to other endangered tetraploid sturgeon species overexploited for caviar production, particularly in regions lacking proper infrastructure and/or expertise. PMID:21483472

  6. Novel technique for visualizing primordial germ cells in sturgeons (Acipenser ruthenus, A. gueldenstaedtii, A. baerii, and Huso huso).

    Science.gov (United States)

    Saito, Taiju; Psenicka, Martin

    2015-10-01

    Primordial germ cells (PGCs) are the origin of all germ cells in developing embryos. In the sturgeon embryo, PGCs develop from the vegetal hemisphere, which mainly acts as an extraembryonic source of nutrition. Current methods for studying sturgeon PGCs require either killing the fish or using costly and time-consuming histological procedures. Here, we demonstrate that visualization of sterlet (Acipenser ruthenus>) PGCs in vivo is feasible by simply labeling the vegetal hemisphere with fluorescein isothiocyanate (FITC)-dextran. We injected FITC-dextrans, with molecular weights varying between 10 000 and 2 000 000, into the vegetal pole of 1- to 4-cell stage embryos. At the neurula to tail-bud developmental stages, FITC-positive PGC-like cells appeared ventrally around the developing tail bud in the experimental group that received a high-molecular-weight FITC-dextran. The highest average number of FITC-positive PGC-like cells was observed in embryos injected with FITC-dextran having a molecular weight of 500 000 (FD-500). The pattern of migration of the labeled cells was identical to that of PGCs, clearly indicating that the FITC-positive PGC-like cells were PGCs. Labeled vegetal cells, except for the PGCs, were digested and excreted before the embryos starting feeding. FITC-labeled PGCs were observed in the developing gonads of fish for at least 3 mo after injection. We also found that FD-500 could be used to visualize PGCs in other sturgeon species. To the best of our knowledge, this report is the first to demonstrate in any animal species that PGCs can be visualized in vivo for a long period by the injection of a simple reagent. PMID:26134864

  7. Environmental contaminants in tissues from an Atlantic sturgeon (Acipenser oxyrinchus) recovered in Wellfleet, Massachusetts

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — On February 2, 2007, a family walking along a Cape Cod beach in Wellfleet, Massachusetts, came upon a large dead sturgeon in the sand. Since very little information...

  8. Contaminants in Atlantic sturgeon and shortnose sturgeon recovered from the Penobscot and Kennebec rivers, Maine.

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — In the Gulf of Maine, the shortnose sturgeon (Acipenser brevirostrum) is a federally‐listed endangered species and the Atlantic sturgeon (Acipenser oxyrinchus) is a...

  9. Impact of the Three Gorges Dam on the spawning stock and natural reproduction of Chinese sturgeon in Changjiang River, China

    Science.gov (United States)

    Gao, Xin; Lin, Pengcheng; Li, Mingzheng; Duan, Zhonghua; Liu, Huanzhang

    2016-01-01

    Chinese sturgeon (Acipenser sinensis) is the flagship species of the Changjiang River. The migration route of this species is blocked by the first dam, the Gezhou Dam, and its reproduction is affected by the Three Gorges Dam (TGD), one of the largest dams in the world. We studied the impact of the impoundment of the Three Gorges Reservoir (TGR) since 2003 on the spawning stock and the natural reproduction of the Chinese sturgeon by using our monitoring data from 1997 to 2013. Results indicate that TGR impoundment has delayed the first spawning dates of the fish from middle-late October to late November, decreased the amount of spawning activities from twice to only once each year, and significantly reduced egg production. In particular, the fish did not demonstrate any spawning activities in 2013. Therefore, TGR impoundment significantly affects the natural reproduction of the fish downstream of the TGD. The spawning stock size of the fish is also predicted to further decrease in the future, which will lead to a risk of population extinction. Ecological regulations must be imposed on decreasing the water temperature to 20°C before mid-October and increasing water discharge downstream of the TGD in October to induce spawning of the Chinese sturgeon.

  10. Impact of the Three Gorges Dam on the spawning stock and natural reproduction of Chinese sturgeon in Changjiang River, China

    Science.gov (United States)

    Gao, Xin; Lin, Pengcheng; Li, Mingzheng; Duan, Zhonghua; Liu, Huanzhang

    2016-09-01

    Chinese sturgeon ( Acipenser sinensis) is the flagship species of the Changjiang River. The migration route of this species is blocked by the first dam, the Gezhou Dam, and its reproduction is affected by the Three Gorges Dam (TGD), one of the largest dams in the world. We studied the impact of the impoundment of the Three Gorges Reservoir (TGR) since 2003 on the spawning stock and the natural reproduction of the Chinese sturgeon by using our monitoring data from 1997 to 2013. Results indicate that TGR impoundment has delayed the first spawning dates of the fish from middle-late October to late November, decreased the amount of spawning activities from twice to only once each year, and significantly reduced egg production. In particular, the fish did not demonstrate any spawning activities in 2013. Therefore, TGR impoundment significantly affects the natural reproduction of the fish downstream of the TGD. The spawning stock size of the fish is also predicted to further decrease in the future, which will lead to a risk of population extinction. Ecological regulations must be imposed on decreasing the water temperature to 20°C before mid-October and increasing water discharge downstream of the TGD in October to induce spawning of the Chinese sturgeon.

  11. Commercial fishing gear modifications to reduce interactions between Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and the southern flounder (Paralichthys lethostigma) fishery in North Carolina (USA).

    Science.gov (United States)

    Levesque, Juan C; Hager, Christian; Diaddorio, Eric; Dickey, R Jason

    2016-01-01

    Bycatch of protected species in commercial fishing operations is a primary concern to fishery managers because it threatens the conservation, protection, and recovery of fragile species, such as the Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus). One potential solution to reduce the risk associated with commercial fishing operations is to design commercial fishing gear that is more selective in terms of interactions between Atlantic sturgeon and commercial fisheries. Given this conservation and management need, the overarching goal was to reduce Atlantic sturgeon fishery interactions and maintain southern flounder (Paralichthys lethostigma) catch in North Carolina. The specific objectives of this study were to design and evaluate the effectiveness of a modified gillnet. Overall, the results proved that lowering the profile and amount of webbing had a beneficial impact at reducing Atlantic sturgeon incidental encounters and bycatch. The modified gillnet reduced bycatch and Atlantic sturgeon encounters by 39.6% and 60.9%, respectively. Our design entangled 51.6% fewer southern flounder, which corresponded to a 48.9% reduction in total weight; the modified gear entangled slightly larger southern flounder than the control gear. Our findings showed the number of Atlantic sturgeon encounters was positively associated with mean water depth, with more Atlantic sturgeon encountered in deeper (5.1-6.3 m) than shallower waters; 75% were encountered at depths between 4.6 and 6.1 m. Most southern flounder (n = 518, 39.7%) were taken at a water depth between 3.76 and 5.0 m. This observation suggests that southern flounder prefer slightly shallower waters than Atlantic sturgeon. PMID:27547524

  12. Effect of anaesthetics MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii)

    Science.gov (United States)

    Feng, G.; Zhuang, P.; Zhang, L.; Kynard, B.; Shi, X.; Duan, M.; Liu, J.; Huang, X.

    2011-01-01

    The effects of MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii) were studied. MS-222 caused higher glucose (GLU) concentrations in anaesthetic test groups than for the control group. Triglyceride (TGL) concentrations of fish in the 140 and 160mgL-1 groups were also significantly higher than those of other groups. Alanine aminotransferase (ALT) activity in the 140mgL-1 group was significantly higher than the level in 80, 100 and 120mgL-1 groups. Aspartate aminotransferase (AST) activity in the 140mgL-1 group was significantly higher than those in the 100 and 120mgL-1 groups. Levels of total protein (TP), cholesterol (CHOL) and alkaline phosphatase (ALP) in anaesthetic test groups were not significantly influenced by MS-222. Clove oil did not have significant effects on levels of GLU, TP, CHOL, ALT and ALP. TGL concentration of fish exposed to 180mgL-1 clove oil was significantly higher than those of the rest anaesthetic groups. AST activities of fish exposed to 120, 150 and 180mgL-1 were significantly higher than those of 60 and 90mgL-1. Overall, TGL and AST could be potentially used as indicators of anaesthetic stress for juvenile Siberian sturgeon. Based on blood biochemical parameters, the appropriate anaesthetic concentrations of MS-222 and clove oil were 80-120mgL-1 and 60-90mgL-1, respectively. Clove oil was a promising alternative to MS-222. ?? 2011 Blackwell Verlag, Berlin.

  13. Columbia River White Sturgeon (Acipenser Transmontanus) Enhancement, May 1-December 31, 1983 Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Brannon, E.L.

    1984-12-01

    Studies were undertaken to examine and define the early life history characteristics of Columbia River white sturgeon as a working base from which enhancement measures could be developed. Adult sturgeon were captured and held for spawning at Covert's Landing, the site of the hatchery facilities below Bonneville Dam. Pituitary hormones stimulated ovulation; ripe females were live spawned surgically and the eggs incubated in hatching jars. Larvae were either reared at the hatchery site after incubation to advanced fingerling stages or transferred to the University laboratory for more detailed study. Displacement downstream occurs as a means of distribution and can last several days before a strong substrate preference is manifested. Once bottom contact is sought by the larvae, displacement is abated, and a general preference for sandy surface appears to predominate. Since potentially extensive displacement downstream could result in the distribution of larvae in saltwater, the tolerance of young sturgeon to saltwater was examined. The responsiveness of young sturgeon to artificial feed was positive. With these results, the original concern for identifying an adequate diet and food source that would be readily accepted by fry was greatly attenuated. The readiness of young fry to initiate feeding on the artificial diet made further study on feeding stimulants unnecessary. Examination of the feeding response suggested that as long as the diet used in the present study was initiated at the proper time and with adequate frequency, the fry would feed quite well and survive. 6 refs., 10 figs., 5 tabs.

  14. Effects of Pediococcus pentosaceus as a probiotic on intestinal microbiota and body composition of Siberian sturgeon, Acipenser baerii Brandt, 1869

    Directory of Open Access Journals (Sweden)

    Fateme Moslehi

    2016-02-01

    Full Text Available An eight-week experiment was carried out to determine the effects of dietary Pediococcus pentosaceus as probiotic on the body composition and gut microbiota of Siberian sturgeon, Acipenser baerii. A total of 180 fish with mean body weight of 143±0.01g were randomly distributed into 12 200L fiberglass tanks as four treatments with three replicates, including groups fed with diet containing 2×107, 2×108 and 2×109 CFU g-1 of P. pentosaceus and probiotic-free as control group. Body composition of treatment groups was not influenced by P. pentosaceus except for fat and moisture. The bacteria had a significant colonization in the intestine of fish fed with supplemented diet with P. pentosaceus. High level of acid lactic bacterial load was found in the treatment fed with highest amount of the probiotic i.e. 2×109 CFU g-1. The results showed that application of P. pentosaceus has positive effect on the body composition and intestinal microflora of the A. baerii.

  15. Determination of the Ovarian Stages in Wild Persian Sturgeon, Acipenser persicus

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    Mahboubeh HOSSENZADE

    2014-09-01

    Full Text Available In the present study we investigated the histological changes in the ovary of 35 female Persian sturgeon. Ovarian samples were taken from the females stained with hematoxylin and eosin (H&E staining and sexual maturity was determined by examining the sections under a light microscope. Four developmental stages of ovary including cortical alveoli formation stage (ΙΙ, vitellogenic stage (ΙII, mature stage (IV and ovulation stage (V were recognized during development. The gonadosomatic index (GSI of female Persian sturgeon gradually increased during the development of ovary. The lowest GSI was recorded in stage II (2.57 ± 0.28 and the highest GSI was shown in stage V (23.58 ± 1.08. Therefore, GSI may be useful to determining maturity stages; however histological experiments of ovaries should be considered as the most accurate method for all stages.doi:10.14456/WJST.2014.80

  16. Interrelationships among egg, larvae and maternal characteristics in Persian sturgeon Acipenser persicus

    OpenAIRE

    Rajab Mohammad Nazari; Mohammad Reza Ghomi; Laura Aquiloni

    2010-01-01

    This study was aimed to observe the correlations among egg, larvae and maternal characteristics of Persian sturgeon. A total of 41 females were used and the variables including fertilization rate, incubation survival rate, larvae survival rate during yolk sac absorption (before feeding), and larvae survival rate after first feeding were determined. As a result, fertilization rate was positively correlated with three variables: incubation survival, larvae survival during yolk sac absorption, a...

  17. Effect of Vegetable Protein and Oil Supplementation on Growth Performance and Body Composition of Russian Sturgeon Juveniles (Acipenser gueldenstaedtii Brandt, 1833) at Low temperatures

    OpenAIRE

    , Erdal Şener; Mustafa Yıldız; (1), Esra Savaş

    2006-01-01

    Effect of different feed compositions on growth performance and body composition of Russian sturgeon (Acipenser gueldenstaedtii) juveniles was studied. One control and three experimental diets were prepared with different ratios of soybean meal (20%, 10.5%, 33%, and 27%) sunflower meal (7%, 8.5%, 14.5%, and 10.5%) and corn meal (10%, 20.5%, 15% and 7.5%) substitute fish meal (40%, 35%, 25%, and 30%) as a protein source. Corn oil (2.5% in Group I and 10% in Group II) and sunflower oil (2.5% in...

  18. Effect of dietary macronutrient proportion on intermediate metabolism and oxidative status in sturgeon (Acipenser naccarii) and trout (Oncorhynchus mykiss): comparative study.

    Science.gov (United States)

    Furné, M; García-Gallego, M; Hidalgo, M C; Sanz, A

    2016-08-01

    Three isoenergetic diets varying the proportion of dietary energy supplied by each of the macronutrients (carbohydrate, lipid, or protein) were delivered, to farmed sturgeon (Acipenser naccarii) and trout (Oncorhynchus mykiss), to test the possible effects on the intermediate metabolism and oxidative status in liver, white muscle, and heart. In trout, there is an adaptive metabolic response to an increase in lipids and carbohydrates in the diet. However, this does not happen in the sturgeon. These differences may be due to different dietary habits of both species. In terms of oxidative status, only the liver displayed oxidative stress in both species, showing an increase in the lipid peroxidation and antioxidant enzyme activities after feeding with the high-lipid and high-protein diet. PMID:26970754

  19. RESEARCH REGARDING THE EVOLUTION OF CORPORAL WEIGHTES AND FOOD CONVERSSION ON JUVENILE OF SIBERIAN STURGEON (Acipenser baeri RAISED IN RECIRCULATING SYSTEM

    Directory of Open Access Journals (Sweden)

    M. BURA

    2013-07-01

    Full Text Available The research was performed within the recirculating aquaculture system for sturgeon growth, in Banat's Universsity of Agricultural Sciences and Veterinary Medicine from Timisoara, on 2851 juveniles of Siberian sturgeons (Acipenser baeri, aged between 132-175 days. Juveniles were maintained in 4 tanks with volume of 5.63 m3, fed with granulated feed of 2 mm (44 & 22% PB and GB. Every 2 weeks the weight and body length was determinate on an effective of 30 individuals from each tank. Based on the obtained body weight, it has been established the food quantity used and bioproductive indicators. Having the weight differences between the individuals of a Siberian sturgeon population, it is needed at certain time intervals to practice assortment by body development. At the age of 175 days, Siberian sturgeons reached an average body weight between 90.13 ± 4.56 g and 197.63 ± 7.22 g and average body length between 28.73 ± 0.43 cm and 37.55 ± 0.38 cm. Individual values of minimum and maximum body weight varied between 32 g and 307 g and body length between 24.5 cm and 42 cm. For the entire population of Siberian sturgeons, there is a middle and high variability for the average body weight and a small variability for the average body length. During the 43 experimental days, the 2851 Siberian sturgeons have acquired a real weight gain of 184.66 kg, with a conversion factor of 0.84 kilograms of feed / kg growth and an index of feed conversion of 1.19 kilograms spore / kg feed consumed.

  20. Researches considering the effect of the gradual insertion of fodder in alimentation for the growth rhythm of Siberian sturgeon larvae (Acipenser baerii – Brandt, 1869) reared in superintensive system

    OpenAIRE

    Daniel OPREA; Lucian OPREA

    2010-01-01

    The experiment was made inside of private sturgeon rearing farm S.C. “Beluga Farm Group” S.R.L. from Calarasi County. The rearing system is flow-through and is emplaced into an enclosure as halltype. Sturgeon larvae were reared in spun glass troughs of a 0.167 m3 capacity. Experiment duration was 20 days. Biological material, Siberian sturgeon larvae (Acipenser baerii – Brandt, 1869), was gained by artificial breeding of breeders imported from Germany. The experiment was going off with two va...

  1. Confirmation of ovarian homogeneity in post-vitellogenic cultured white sturgeon, Acipenser transmontanus.

    Science.gov (United States)

    Talbott, Mariah J; Servid, Sarah A; Cavinato, Anna G; Van Eenennaam, Joel P; Doroshov, Serge I; Struffenegger, Peter; Webb, Molly A H

    2014-02-01

    Assessing stage of oocyte maturity in female sturgeon by calculating oocyte polarization index (PI) is a necessary tool for both conservation propagation managers and caviar producers to know when to hormonally induce spawning. We tested the assumption that sampling ovarian follicles from one section of one ovary is sufficient for calculating an oocyte PI representative of oocyte maturity for an individual animal. Short-wavelength near-infrared spectroscopy (SW-NIR) scans were performed on three positions per ovary for five fish prior to caviar harvest. Samples of ovarian follicles were subsequently taken from the exact location of the SW-NIR scans for calculation of oocyte PI and follicle diameter. Oocyte PI was statistically different though not biologically relevant within an ovary and between ovaries in four of five fish. Follicle diameter was statistically different but not biologically relevant within an ovary in three of five fish. There were no differences in follicle diameter between ovaries. No statistical differences were observed between SW-NIR spectra collected at different locations within an ovary or between ovaries. These results emphasize the importance of utilizing both oocyte PI measurement and progesterone-induced oocyte maturation assays while deciding when to hormonally induce spawning in sturgeon females. PMID:24174166

  2. The inner ear morphology and hearing abilities of the Paddlefish (Polyodon spathula) and the Lake Sturgeon (Acipenser fulvescens).

    Science.gov (United States)

    Lovell, J M; Findlay, M M; Moate, R M; Nedwell, J R; Pegg, M A

    2005-11-01

    Concern regarding the spread of silver carp (Hypopthalmichthys molitrix) and bighead carp (Aristichthysc nobilis) through the Illinois River has prompted the development of an Acoustic Fish Deterrent (AFD) system. The application of this technology has resulted in a need to understand the auditory physiology of fish other than the target species, in order to minimise the effect of the AFD barrier on the ecology of indigenous fish populations. To this end, both the structures involved in sound reception and the hearing abilities of the paddlefish (Polyodon spathula) and the lake sturgeon (Acipenser fulvescens) are studied here using a combination of morphological and physiological approaches, revealing that both fish are responsive to sounds ranging in frequency from 100 to 500 Hz. The lowest hearing thresholds from both species were acquired from frequencies in a bandwidth of between 200 and 300 Hz, with higher thresholds at 100 and 500 Hz. The rationale for studying hearing in P. spathula and A. fulvescens in particular, is the value placed on them by both the commercial caviar producing industry and by the recreational fisheries sector. The hearing abilities of twelve P. spathula and twelve A. fulvescens were tested in sound fields dominated by either sound pressure or particle motion, with the results showing that acipenseriform fish are responsive to the motion of water particles in a sound field, rather than the sound pressure component. In this study, we measure the intensity of the sound field required to evoke threshold responses using a pressure sensitive hydrophone, as pressure dominated sound fields are the most audible acoustic condition for specialists like H. molitrix and A. nobilis (the target species). The results of the auditory examination clearly show that P. spathula and A. fulvescens are not sensitive to sound pressure, and will therefore have a significantly higher deterrent threshold than H. molitrix and A. nobilis in a pressure dominated

  3. Biomarker responses in persian sturgeon (Acipenser persicus exposed to benzo-a-pyrene and beta-naphthoflavone

    Directory of Open Access Journals (Sweden)

    Karimzadeh Katayoon

    2013-01-01

    Full Text Available Biotransformation enzymes of xenobiotics (ethoxyresorufin-O-deethylase, cytochrome P4501A1 content and glutathione-S-transferase were investigated in the liver of Persian Sturgeon (Acipenser persicus after a 96-hour exposure to polycyclic aromatic hydrocarbons (PAHs, premutagenic benzo[a]pyrene (BaP and beta-naphthoflavone (BNF. The fish were injected 10 mg/kg wet-body weight in corn oil for 96 hours every days. Ethoxyresorufin-O-deethylase activity (EROD and glutathione s-transferase activity (GST were measured in the fish liver. Cytochrome P4501A1 (CYP1A1 content was estimated by indirect enzyme-linked immunosorbent assay (ELISA. The response appeared as early as 12 hours post exposure. A time-dependent response was observed in the EROD activity, being significantly higher at 48 hours post exposure to 10 mg/kg of BaP. The greatest induction occurred in the fish treated with 10 mg/kg BaP, in which a 32.1- fold increase in EROD activity was observed. Results showed that EROD activity in A. persicus is significantly increased by BaP and BNF treatments. Both chemicals showed higher values of EROD activity compared to the liver CYP1A content. There was a rise in glutathione-S-transferase activity in fish exposed to BNF, but no increase was observed in fish treated with BaP. The results showed that hepatic CYP1A expression in terms of induction of EROD activity might be suited as a biomarker of organic contamination in aquatic environments and led to lower sensitivity of the second phase in the detoxification enzyme.

  4. Acute and chronic sensitivity of white sturgeon (Acipenser transmontanus) and rainbow trout (Oncorhynchus mykiss) to cadmium, copper, lead, or zinc in laboratory water-only exposures

    Science.gov (United States)

    Ingersoll, Christopher G.; Contributions by Wang, Ning; Calfee, Robin D.; Beahan, Erinn; Brumbaugh, William G.; Dorman, Rebecca A.; Hardesty, Doug K.; Kunz, James L.; Little, Edward E.; Mebane, Christopher A.; Puglis, Holly J.

    2014-01-01

    White sturgeon (Acipenser transmontanus) are experiencing poor recruitment in the trans boundary reach of the upper Columbia River in eastern Washington State. Limited toxicity data indicated that early life stages of white sturgeon are sensitive to metals. In acute 4-day (d) exposures with larval white sturgeon, previous studies have reported that the 4-day median lethal concentrations (LC50) based on biotic ligand model (BLM) normalization for copper were below the U.S. Environmental Protection Agency national recommended acute water-quality criterion. In previously published chronic 66-d exposures starting with newly fertilized eggs of white sturgeon, 20-percent lethal effect concentrations (LC20s) for copper, cadmium, or zinc generally were within a factor of two of the chronic values of the most sensitive fish species in the databases of the U.S. Environmental Protection Agency water-quality criteria (WQC) for the three metals. However, there were some uncertainties in the chronic exposures previously performed with white sturgeon, including (1) low control survival (37 percent), (2) more control fish tested in each replicate compared to other treatments, (3) limited replication of treatments (n=2), (4) lack of reported growth data (such as dry weight), and (5) wide dilution factors for exposure concentrations (6- to 8-fold dilutions). The U.S. Environmental Protection Agency concluded that additional studies are needed to generate more toxicity data to better define lethal and sublethal toxicity thresholds for metals for white sturgeon. The objective of the study was to further evaluate the acute and chronic toxicity of cadmium, copper, lead, or zinc to early life stages of white sturgeon in water-only exposures. Toxicity tests also were performed with commonly tested rainbow trout (Oncorhynchus mykiss) under similar test conditions to determine the relative sensitivity between white sturgeon and rainbow trout to these metals. Toxicity data generated from

  5. Acute sensitivity of white sturgeon (Acipenser transmontanus) and rainbow trout (Oncorhynchus mykiss) to copper, cadmium, or zinc in water-only laboratory exposures

    Science.gov (United States)

    Calfee, Robin D.; Little, Edward E.; Puglis, Holly J.; Scott, Erinn L.; Brumbaugh, William G.; Mebane, Christopher A.

    2014-01-01

    The acute toxicity of cadmium, copper, and zinc to white sturgeon (Acipenser transmontanus) and rainbow trout (Oncorhynchus mykiss) were determined for 7 developmental life stages in flow-through water-only exposures. Metal toxicity varied by species and by life stage. Rainbow trout were more sensitive to cadmium than white sturgeon across all life stages, with median effect concentrations (hardness-normalized EC50s) ranging from 1.47 µg Cd/L to 2.62 µg Cd/L with sensitivity remaining consistent during later stages of development. Rainbow trout at 46 d posthatch (dph) ranked at the 2nd percentile of a compiled database for Cd species sensitivity distribution with an EC50 of 1.46 µg Cd/L and 72 dph sturgeon ranked at the 19th percentile (EC50 of 3.02 µg Cd/L). White sturgeon were more sensitive to copper than rainbow trout in 5 of the 7 life stages tested with biotic ligand model (BLM)-normalized EC50s ranging from 1.51 µg Cu/L to 21.9 µg Cu/L. In turn, rainbow trout at 74 dph and 95 dph were more sensitive to copper than white sturgeon at 72 dph and 89 dph, indicating sturgeon become more tolerant in older life stages, whereas older trout become more sensitive to copper exposure. White sturgeon at 2 dph, 16 dph, and 30 dph ranked in the lower percentiles of a compiled database for copper species sensitivity distribution, ranking at the 3rd (2 dph), 5th (16 dph), and 10th (30 dph) percentiles. White sturgeon were more sensitive to zinc than rainbow trout for 1 out of 7 life stages tested (2 dph with an biotic ligand model–normalized EC50 of 209 µg Zn/L) and ranked in the 1st percentile of a compiled database for zinc species sensitivity distribution.

  6. Growth Performance and Feed Utilization of Russian Sturgeon Acipenser gueldenstaedtii Brandt&Ratzeburg, 1833 in Grow-out Phase Cultured in the Black Sea

    Directory of Open Access Journals (Sweden)

    Mehmet Kocabaş

    2015-10-01

    Full Text Available The aim of present study was to determine the growth performance of Russian sturgeon Acipenser gueldenstaedtii throughout 5 years and to investigate problems encountered in grow-out phase. Fish samples (2750.88±351.18 g, 80.18±6.49 cm mean±SD were equally stocked into fiberglass tanks, three replicate per treatment and fed for 5 years. Gain of weight and length, Specific Growth Rate (SGR, Condition Factor (CF and Feed Conversion Ratio (FCR were determined. Our results indicated that mean final length and weight were 106.34±2.79 (cm and 6651.16±3.16 (g. The mean condition factor (CF at year 5 was 0.51. FCR values fluctuated and the highest value of food conversion rate (FCR was determined in 4th year. During the rearing period of Russian sturgeon, SGR values decreased dramatically from 2.88 to 1.83. In conclusion, slow growth was determined in Russian sturgeon A. gueldenstaedtii. Therefore, there is need for extensive evaluation and comparison of data obtained from field studies and those obtained from laboratory studies.

  7. Can fatty acid and mineral compositions of sturgeon eggs distinguish between farm-raised versus wild white (Acipenser transmontanus) sturgeon origins in California? Preliminary report.

    Science.gov (United States)

    DePeters, Edward J; Puschner, Birgit; Taylor, Scott J; Rodzen, Jeff A

    2013-06-10

    The objective was to investigate the potential of using fatty acid and mineral compositions of sturgeon eggs to distinguish their source, either farm-raised or wild fish. Trafficking of illegally obtained wild white sturgeon eggs is a major concern to the California Department of Fish and Game, but there is no forensic method to separate wild and farm-raised white sturgeon eggs. The extension of these findings in future work will be to use the fatty acid and mineral compositions as forensic indicators of caviar produced legally from farm raised sturgeon compared with illegal caviar produced from sturgeon poached from the wild. Samples (10) of sturgeon eggs were collected from a commercial aquaculture facility in the Sacramento Valley. Eggs from wild sturgeon (9) were obtained primarily from confiscations of illegally caught sturgeon by fish and game law enforcement personnel. The total lipid content of sturgeon eggs was analyzed for fatty acid composition. The most notable difference was the higher concentration (Pcaviar origin. PMID:23683918

  8. Bioaccumulation of Zn, Cu and Mn in the caviar and muscle of Persian sturgeon (Acipenser persicus) from the Caspian Sea, Iran.

    Science.gov (United States)

    Mashroofeh, Abdulreza; Bakhtiari, Alireza Riyahi; Pourkazemi, Mohammad

    2012-12-01

    Concentrations of Zn, Cu and Mn were examined in caviar and muscle of the Persian sturgeon (Acipenser persicus) collected from coastal waters of south Caspian Sea during March and April, 2011. Mean Zn, Cu and Mn concentrations in caviar samples were 21.48, 2.05 and 1.66 μg g(-1) wet weight basis, respectively. The mean Zn, Cu and Mn concentrations in muscle tissues were 7.49, 1.00 and 0.34 μg g(-1) wet weight basis, respectively. The mean concentrations of Zn and Cu in caviar and muscle samples were under the permissible limits proposed by the United Kingdom's Ministry of Agriculture, Fisheries and Food (2000). PMID:23080537

  9. Siberian sturgeon (Acipenser baerii) larvae fed Artemia nauplii enriched with poly-beta-hydroxybutyrate (PHB): effect on growth performance, body composition, digestive enzymes, gut microbial community, gut histology and stress tests

    OpenAIRE

    Najdegerami, H; K. Baruah; Shiri, A.; Rekecki, A.; Van Den Broeck, W.; Sorgeloos, P.; Boon, N.; P. Bossier; De Schryver, P.

    2015-01-01

    The effects of feeding Artemia nauplii enriched with or without poly-ß-hydroxybutyrate (PHB) and/or highly unsaturated fatty acids (HUFA) on Siberian sturgeon (Acipenser baerii) larvae were investigated. Feeding larvae with PHB-enriched nauplii (PHB, PHB + HUFA) decreased the growth performance of the larvae. PHB affected the body composition by increasing the lipid content of the whole body and decreasing total saturated, monoenoic, n3, n6 and decosahexanoeic acid (DHA) in the larvae, indica...

  10. Characterization of the contents and histology of the gastrointestinal tracts of White Sturgeon (Acipenser transmontanus) captured from Upper Lake Roosevelt, Washington, October 2008

    Science.gov (United States)

    Parsley, Michael J.; van der Leeuw, Bjorn K.; Elliott, Diane G.

    2010-01-01

    The gastrointestinal tracts of 37 juvenile white sturgeon (Acipenser transmontanus) captured from the upper part of Lake Roosevelt during October 2008, were examined to identify prey taxa and to determine if the fish were consuming smelter slag along with other sediments. Histological examination of the gastrointestinal tract tissues and comparison with similar tissues from hatchery-reared fish also was performed. The contents of the gastro-intestinal tracts (guts) indicated that white sturgeon were actively foraging on various benthic invertebrates and the diet was quite diverse, with more than 50 percent of the fish feeding on five or more different taxa. Slag was present in 76 percent of the guts examined. Although not all guts contained slag particles, larger fish tended to have greater amounts of slag in their guts. Histology of the gut tissues showed the presence of a chronic inflammatory response, and the severity of the response had a significant positive correlation (P = 0.01) with fish length and weight suggesting that the inflammation represented a response to long-term exposure to one or more stressors. However, additional work is needed to determine if the physical or chemical properties of slag contributed to this response.

  11. Effect of dietary supplementation of fructooligosaccharide (FOS) on growth performance, survival, lactobacillus bacterial population and hemato-immunological parameters of stellate sturgeon (Acipenser stellatus) juvenile.

    Science.gov (United States)

    Akrami, Reza; Iri, Yousef; Rostami, Hosseinali Khoshbavar; Razeghi Mansour, Majid

    2013-10-01

    The dietary supplementation of fructooligosaccharide (FOS) in stellate sturgeon juvenile, Acipenser stellatus (with mean initial body weight of 30.16 ± 0.14 g) was evaluated for the effect on growth, autochthonous intestinal microbiata and hemato-immunological parameters for 11 weeks. FOS was added at a level of 0, 1% and 2% to the commercial pellet diet (BioMar). At the end of the experiment, growth parameters, survival rate, lactobacillus bacterial population, hematological and immunological parameters were determined. The fish fed on 1% FOS significantly showed higher final weight, WG%, SGR and PER and lower FCR compared to those of the control group (P  0.05). However, FOS administration resulted in lower survival. The serum lysozyme activity was significantly affected by dietary 1% FOS (P  0.05). In fish fed on the diet with 1% FOS showed a significant increase of total heterotrophic autochthonous bacterial and presumptive LAB levels (P prebiotics. In addition to increase in WBC, RBC, MCV, hematocrit, hemoglobin and lymphocyte levels were observed in this group. These results indicated that dietary supplementation of FOS at a dose of 1% improved growth performance, beneficial intestinal microbiata and stimulate immune response of stellate sturgeon juvenile. PMID:23973846

  12. White Sturgeon Bibliography, 1985 Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Fickeisen, Duane H. (Pacific Northwest National Laboratory, Richland, WA)

    1986-03-01

    This bibliography presents citations to the majority of published materials on white sturgeon (Acipenser transmontanus). The purpose was to assist in planning and implementing research on white sturgeon in the Columbia River system. (ACR)

  13. The effects of commercial diets on performance, muscle proximate composition and waste loads of white sturgeon (Acipenser transmontanus

    Directory of Open Access Journals (Sweden)

    S. Garavello

    2011-03-01

    Full Text Available The rearing of sturgeons has shown a considerable increase in recent years, also in Italy (Ronyai and Varadi,1995, both for the caviar and for the meat, under increasing demand (Williot and Bourguignon,1991. There is still little information on the nutrient requirements and optimal feeding rates of white sturgeon (Hung, 1991, even if specific diets for this species are now available on market...

  14. Integrating Water Flow, Locomotor Performance and Respiration of Chinese Sturgeon during Multiple Fatigue-Recovery Cycles

    OpenAIRE

    Cai, Lu; Chen, Lei; Johnson, David; Gao, Yong; Mandal, Prashant; Fang, Min; Tu, Zhiying; Huang, Yingping

    2014-01-01

    The objective of this study is to provide information on metabolic changes occurring in Chinese sturgeon (an ecologically important endangered fish) subjected to repeated cycles of fatigue and recovery and the effect on swimming capability. Fatigue-recovery cycles likely occur when fish are moving through the fishways of large dams and the results of this investigation are important for fishway design and conservation of wild Chinese sturgeon populations. A series of four stepped velocity tes...

  15. Evidence of circadian rhythm, oxygen regulation capacity, metabolic repeatability and positive correlations between forced and spontaneous maximal metabolic rates in lake sturgeon Acipenser fulvescens.

    Directory of Open Access Journals (Sweden)

    Jon C Svendsen

    Full Text Available Animal metabolic rate is variable and may be affected by endogenous and exogenous factors, but such relationships remain poorly understood in many primitive fishes, including members of the family Acipenseridae (sturgeons. Using juvenile lake sturgeon (Acipenser fulvescens, the objective of this study was to test four hypotheses: 1 A. fulvescens exhibits a circadian rhythm influencing metabolic rate and behaviour; 2 A. fulvescens has the capacity to regulate metabolic rate when exposed to environmental hypoxia; 3 measurements of forced maximum metabolic rate (MMR(F are repeatable in individual fish; and 4 MMR(F correlates positively with spontaneous maximum metabolic rate (MMR(S. Metabolic rates were measured using intermittent flow respirometry, and a standard chase protocol was employed to elicit MMR(F. Trials lasting 24 h were used to measure standard metabolic rate (SMR and MMR(S. Repeatability and correlations between MMR(F and MMR(S were analyzed using residual body mass corrected values. Results revealed that A. fulvescens exhibit a circadian rhythm in metabolic rate, with metabolism peaking at dawn. SMR was unaffected by hypoxia (30% air saturation (O(2sat, demonstrating oxygen regulation. In contrast, MMR(F was affected by hypoxia and decreased across the range from 100% O(2sat to 70% O(2sat. MMR(F was repeatable in individual fish, and MMR(F correlated positively with MMR(S, but the relationships between MMR(F and MMR(S were only revealed in fish exposed to hypoxia or 24 h constant light (i.e. environmental stressor. Our study provides evidence that the physiology of A. fulvescens is influenced by a circadian rhythm and suggests that A. fulvescens is an oxygen regulator, like most teleost fish. Finally, metabolic repeatability and positive correlations between MMR(F and MMR(S support the conjecture that MMR(F represents a measure of organism performance that could be a target of natural selection.

  16. Neomycin damage and regeneration of hair cells in both mechanoreceptor and electroreceptor lateral line organs of the larval Siberian sturgeon (Acipenser baerii).

    Science.gov (United States)

    Fan, Chunxin; Zou, Sha; Wang, Jian; Zhang, Bo; Song, Jiakun

    2016-05-01

    The lateral line found in some amphibians and fishes has two distinctive classes of sensory organs: mechanoreceptors (neuromasts) and electroreceptors (ampullary organs). Hair cells in neuromasts can be damaged by aminoglycoside antibiotics and they will regenerate rapidly afterward. Aminoglycoside sensitivity and the capacity for regeneration have not been investigated in ampullary organs. We treated Siberian sturgeon (Acipenser baerii) larvae with neomycin and observed loss and regeneration of sensory hair cells in both organs by labeling with DASPEI and scanning electron microscopy (SEM). The numbers of sensory hair cells in both organs were reduced to the lowest levels at 6 hours posttreatment (hpt). New sensory hair cells began to appear at 12 hpt and were regenerated completely in 7 days. To reveal the possible mechanism for ampullary hair cell regeneration, we analyzed cell proliferation and the expression of neural placodal gene eya1 during regeneration. Both cell proliferation and eya1 expression were concentrated in peripheral mantle cells and both increased to the highest level at 12 hpt, which is consistent with the time course for regeneration of the ampullary hair cells. Furthermore, we used Texas Red-conjugated gentamicin in an uptake assay following pretreatment with a cation channel blocker (amiloride) and found that entry of the antibiotic was suppressed in both organs. Together, our results indicate that ampullary hair cells in Siberian sturgeon larvae can be damaged by neomycin exposure and they can regenerate rapidly. We suggest that the mechanisms for aminoglycoside uptake and hair cell regeneration are conserved for mechanoreceptors and electroreceptors. J. Comp. Neurol. 524:1443-1456, 2016. © 2015 Wiley Periodicals, Inc. PMID:26502298

  17. Conservation, sex-biased expression and functional annotation of microRNAs in the gonad of Amur sturgeon (Acipenser schrenckii).

    Science.gov (United States)

    Zhang, Xiujuan; Yuan, Lihong; Li, Linmiao; Jiang, Haiying; Chen, Jinping

    2016-06-01

    MicroRNAs (miRNAs) are involved in post-transcriptional gene regulation and have crucial roles in regulating the expression of gametogenesis-related genes in animals. However, the mechanism of sex determination and differentiation in the sturgeon has remained unclear. Identifying miRNAs and characterizing sex-biased miRNA expression is therefore critical for understanding the role of miRNAs during sexual differentiation in sturgeon. In this study, five different tissues from sturgeon before sex differentiation and the gonads were used for miRNA expression profiling. We screened 1037 miRNAs in miRBase 20.0 and an additional 103 sturgeon miRNAs using microarray and real-time PCR. We found that the sequences of 477 miRNAs out of a total of 1140 miRNAs were highly conserved (100%) among different fish species. From a total of 663 non-redundant miRNA probes, 481 miRNAs were detected in the gonads of both sexes. Of the 148 miRNAs that were identified to have sex-biased expression patterns between the testis and the ovary (P2. The microarray expression patterns of 13 randomly selected sex-biased miRNAs were validated using real-time PCR. Target gene prediction revealed a significant enrichment of functional groups (88 GO terms) and 18 KEGG pathways (P<0.05) and suggested that there are interactions between sex-biased miRNAs and 25 putative gametogenesis-related targets. Therefore, our miRNA expression analysis in juvenile A. schrenckii establishes a foundation for understanding and further investigating the role of miRNAs in sturgeon sex differentiation. PMID:27089517

  18. Desarrollo Embrionario de Acipenser naccarii_video

    OpenAIRE

    Cabrera, Remedios

    2016-01-01

    Este video en inglés se encuentra como material adicional del artículo: "Embryonic Development of Adriatic Sturgeon, Acipenser naccarii (Bonaparte 1836) in farming conditions: a guide". Acta Zoologica, 2015.

  19. Análisis molecular de Acipenser sturio L., 1758 y Acipenser oxyrinchus Mitchill, 1815: una revisión

    OpenAIRE

    Birstein, V. J.; Doukakis, P.

    2001-01-01

    Molecular phylogeny based on the analysis of partial sequences of mitochondrial (mt) cytochrome b (cyt b), 12S, and 16S genes supports a close relationship between the European Atlantic sturgeon Acipenser sturio L., 1758, and the American Atlantic sturgeon, Acipenser oxyrinchus Mitchill, 1815. These sister species form a separate clade within the genus Acipenser and appear to be the most basal clade with respect to all other species of the genus. Intraspecifically, at least two different gene...

  20. Sperm-cell ultrastructure of North American sturgeons. IV. The pallid sturgeon (Scaphirhynchus albus Forbes and Richardson, 1905)

    Science.gov (United States)

    DiLauro, M.N.; Walsh, R.A.; Peiffer, M.; Bennett, R.M.

    2001-01-01

    Sperm-cell morphology and ultrastructure in the pallid sturgeon (Scaphirhynchus albus) were examined using transmission and scanning electron microscopy. Metrics and structure were compared with similar metrics obtained from other published descriptions of sturgeon sperm cells. General morphology was found to be similar to that of sperm cells of the white (Acipenser transmontanus), lake (A. fulvescens), stellate (A. stellatus), Chinese (A. sinensis), Russian (A. gueldenstaedti colchicus), and shortnose (A. brevirostrum) sturgeons, which all shared a gradual tapering of the nuclear diameter from posterior to anterior, unlike that of the Atlantic sturgeon (A. oxyrhynchus). The sperm cell of the pallid sturgeon was similar in size to that of the Atlantic sturgeon, being only slightly larger. The sperm cell of the pallid sturgeon differed from those of other sturgeons chiefly in the acrosomal region, where the posterolateral projections (PLP) have the shape of an acute triangle and are arranged in a spiral about the longitudinal axis of the cell. The PLP were longer than those of other sturgeons, being twice the length of those of the Atlantic sturgeon and 58% longer than those of the lake sturgeon. Also, in cross section the acrosome had the shape of a hollow cone rather than the cap of an oak tree acorn, as was found in ultrastructural studies of other sturgeons. In addition, we were able to confirm that the structural arrangement of the distal centriole of the midpiece is identical with that of the proximal centriole: nine sets of microtubular triplets around the periphery of the centriole. This information is of potential use to fishery biologists, forensic biologists, zoologists, reproductive physiologists, taxonomists, evolutionary biologists, and aquaculturists.

  1. Integrating water flow, locomotor performance and respiration of Chinese sturgeon during multiple fatigue-recovery cycles.

    Directory of Open Access Journals (Sweden)

    Lu Cai

    Full Text Available The objective of this study is to provide information on metabolic changes occurring in Chinese sturgeon (an ecologically important endangered fish subjected to repeated cycles of fatigue and recovery and the effect on swimming capability. Fatigue-recovery cycles likely occur when fish are moving through the fishways of large dams and the results of this investigation are important for fishway design and conservation of wild Chinese sturgeon populations. A series of four stepped velocity tests were carried out successively in a Steffensen-type swimming respirometer and the effects of repeated fatigue-recovery on swimming capability and metabolism were measured. Significant results include: (1 critical swimming speed decreased from 4.34 bl/s to 2.98 bl/s; (2 active oxygen consumption (i.e. the difference between total oxygen consumption and routine oxygen consumption decreased from 1175 mgO2/kg to 341 mgO2/kg and was the primary reason for the decrease in Ucrit; (3 excess post-exercise oxygen consumption decreased from 36 mgO2/kg to 22 mgO2/kg; (4 with repeated step tests, white muscle (anaerobic metabolism began contributing to propulsion at lower swimming speeds. Therefore, Chinese sturgeon conserve energy by swimming efficiently and have high fatigue recovery capability. These results contribute to our understanding of the physiology of the Chinese sturgeon and support the conservation efforts of wild populations of this important species.

  2. Researches considering the effect of the gradual insertion of fodder in alimentation for the growth rhythm of Siberian sturgeon larvae (Acipenser baerii – Brandt, 1869 reared in superintensive system

    Directory of Open Access Journals (Sweden)

    Daniel OPREA

    2010-08-01

    Full Text Available The experiment was made inside of private sturgeon rearing farm S.C. “Beluga Farm Group” S.R.L. from Calarasi County. The rearing system is flow-through and is emplaced into an enclosure as halltype. Sturgeon larvae were reared in spun glass troughs of a 0.167 m3 capacity. Experiment duration was 20 days. Biological material, Siberian sturgeon larvae (Acipenser baerii – Brandt, 1869, was gained by artificial breeding of breeders imported from Germany. The experiment was going off with two variants and three replications each. The rearing density was the same in both experimental variants, 500 ex/tank respectively. The diet of larvae from each variant was different as follows: in variant I, the larvae were fed exclusively on live diet represented by Tubifex species; in variant II, the larvae were gradually carried over from a diet consisting of Tubifex species to a diet represented only by Perla Larva fodder with a protean content of 62.0 %. On both experimental variants, fed delivery was achieved in the same way, namely the same number of daily intakes, dispensed at the same time. At the end of theexperiment, the results were as follows: in variant I, the survival rate was 75 % and the final average body mass of larvae 2.69 g/ex, in variant II, the survival rate was 66 % and the final average body mass2.38 g/ex.

  3. Environmental contaminants in shortnose sturgeon from Bears Bluff National Fish Hatchery, Wadmalaw Island, South Carolina

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The shortnose sturgeon (Acipenser brevirostrum) is a federally‐listed endangered species. In 2008, eleven shortnose sturgeon, reared at the Bears Bluff National...

  4. A habitat suitability model for Chinese sturgeon determined using the generalized additive method

    Science.gov (United States)

    Yi, Yujun; Sun, Jie; Zhang, Shanghong

    2016-03-01

    The Chinese sturgeon is a type of large anadromous fish that migrates between the ocean and rivers. Because of the construction of dams, this sturgeon's migration path has been cut off, and this species currently is on the verge of extinction. Simulating suitable environmental conditions for spawning followed by repairing or rebuilding its spawning grounds are effective ways to protect this species. Various habitat suitability models based on expert knowledge have been used to evaluate the suitability of spawning habitat. In this study, a two-dimensional hydraulic simulation is used to inform a habitat suitability model based on the generalized additive method (GAM). The GAM is based on real data. The values of water depth and velocity are calculated first via the hydrodynamic model and later applied in the GAM. The final habitat suitability model is validated using the catch per unit effort (CPUEd) data of 1999 and 2003. The model results show that a velocity of 1.06-1.56 m/s and a depth of 13.33-20.33 m are highly suitable ranges for the Chinese sturgeon to spawn. The hydraulic habitat suitability indexes (HHSI) for seven discharges (4000; 9000; 12,000; 16,000; 20,000; 30,000; and 40,000 m3/s) are calculated to evaluate integrated habitat suitability. The results show that the integrated habitat suitability reaches its highest value at a discharge of 16,000 m3/s. This study is the first to apply a GAM to evaluate the suitability of spawning grounds for the Chinese sturgeon. The study provides a reference for the identification of potential spawning grounds in the entire basin.

  5. Columbia River White Sturgeon (Acipenser Transmontanus) Population Genetics and Early Life History Study, January 1, 1986 to December 31, 1986, Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Brannon, Ernest L.

    1986-12-01

    The 1986 Columbia River white sturgeon investigations continued to assess genetic variability of sturgeon populations isolated in various areas of the Columbia River, and to examine environmental factors in the habitat that may affect early life history success. Baseline data have been collected for three character sets. Twenty-eight loci have been analyzed for differences using electrophoresis, snout shapes were assessed for multivariate distinction, and scute counts have been examined as an index of variability. Fish that reside in the mid-Columbia and lower river have been sufficiently characterized by electrophoresis to compare with up-river areas. To date, few electrophoretic differences have been identified. However, Lake Roosevelt sturgeon sample size will be increased to determine if some of the observed differences from lower river fish are significant. Snout shape has been shown to be easily quantifiable using the digitizing technique. Scute count data initially indicate that variability exists within as well as between areas. Patterns of differentiation of one or more of these data sets may be used to formulate stock transplant guidelines essential for proper management or enhancement of this species. The historical habitat available to sturgeon in the Columbia River has changed through the development of hydroelectric projects. Dams have reduced the velocity and turbulence, and increased light penetration in the water column from less silt. These changes have affected the ability of sturgeon to feed and have made them more vulnerable to predation, which appear to have altered the ability of populations isolated in the reservoirs to sustain themselves. Present studies support the theory that both the biological and physical habitat characteristics of the Columbia River are responsible for reduced sturgeon survival, and justify consideration of enhancement initiatives above Bonneville to improve sturgeon reproductive success.

  6. Characterization of type I and II procollagen α1 chain in Amur sturgeon (Acipenser schrenckii) and comparison of their gene expression.

    Science.gov (United States)

    Zhang, Xi; Azuma, Noriko; Hagihara, Seishi; Adachi, Shinji; Ura, Kazuhiro; Takagi, Yasuaki

    2016-03-15

    To characterize type I and II collagen in the Amur sturgeon at the molecular level, mRNAs encoding the proα chain of both types of collagen were cloned and sequenced. Full sequences of both were obtained, and the molecular phylogeny based on the deduced amino acid sequence indicated that the correct sequences of the target genes were obtained. Analyses of primary structure of the proα chains revealed that type I and II collagen share the basic structure of the proα chain of fibril collagen, but have different characteristics, especially in residues related to thermal stability. In the triple helical domain, Gly-Pro-Pro sequence stabilizing the tripeptide unit was more frequent in type II than in type I, and Gly-Gly, which likely decline in thermal stability, was more frequent in type I than in type II. These results suggested that the denaturation temperature of type II would be remarkably higher than type I. The spatial pattern of gene expression was analyzed by quantitative real-time PCR, which showed that relatively ubiquitous type I gene and strongly skewed distribution of type II gene, which highly expressed only in vertebra, snout cartilage, and notochord. This pattern was similar to the distribution pattern of each collagen protein detected by previous biochemical analyses using Amur and Bester sturgeons. The present study is the first report of the cloning of the full-length cDNAs for both of type I and type II collagen in the Amur sturgeon, and is the first comparative analysis of type I and II collagens in a sturgeon species at the molecular level. The results provide basic and general information on collagens in sturgeons. PMID:26768575

  7. Columbia River White Sturgeon (Acipenser Transmontanus) Early Life History and Genertics Study, August 1, 1984 to December 31, 1985 Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Brannon, Ernest L.

    1985-12-01

    Research on Columbia River white sturgeon has been directed at their early life history as it may apply to production and enhancement strategies for management of the species. The river environment in which sturgeon historically migrated, spawned, and reared has changed through development. Habitat changes are expected to precipitate genetic changes in the fish, as well as reduce the fitness in populations. Genetic analysis of samples taken from various locations over the length of the Columbia River have indicated that observed gene frequencies in all areas sampled were not in Hardy-Weinburg equilibrium, which could suggest that the general population is experiencing perturbation in the system. Analysis thus far has exposed few differences between samples from the lower, middle, and upper portions of the system. Allelic differences were identified in fish from the Roosevelt Lake, which may be evidence of unique characteristics among fish from that general area.

  8. Concentrations of Cd, Ni, V and Zn in Muscle and Caviar of Persian Sturgeon (Acipenser persicus with Emphasis on Risk Assessment Due to Consumption of Muscle

    Directory of Open Access Journals (Sweden)

    Abdulreza Mashroofeh

    2014-03-01

    Material and Methods: A total number of 24 samples of Persian sturgeon were collected from two important sturgeon fishery zones in Guilan and Mazandaran Provinces and were stored at −20°C until chemical analysis. About one gram of each sample dried was added to 10 ml of concentrated (65% supra-pure HNO3 (Merck, Darmstadt, Germany in a Teflon PTFE tube and then it was incubated for 1 h at 40 °C in a hot block digester, followed by heating at 140 °C for 3 h. The samples digested were diluted to a measured volume using double deionized water. Samples were filtered through Whatman No. 1 filter paper, and the filtrate was stored until metal determination. Results: Mean Cd, Ni, V and Zn concentrations in muscle tissues were 0.005 ± 0.002, 0.06 ± 0.03, 0.13 ± 0.04 and 7.49 ± 3.07 µg/g wet weight basis respectively. The mean Cd, Ni, V and Zn concentrations in caviar samples were 0.007 ± 0.006, 0.09 ± 0.07, 0.12 ± 0.15 and 21.23 ± 5.75 µg/g wet weight basis respectively. Conculation: The mean concentrations of Zn, Cd and V in caviar and muscle samples were less than the permissible limits proposed by the United Kingdom's Ministry of Agriculture, Fisheries and Food (MAFF 2000 and World Health Organization (W.H.O.. The guidelines for Cd, V and Zn are 0.2, 0.5 and 50 µg/g wet weight respectively. Therefore, the measured concentrations for these particular metals are below the published guidelines, and the levels would appear not to constitute any threat to the human population that may consume sturgeon or caviar obtained from these study locations.

  9. Distinguishing ovarian maturity of farmed white sturgeon (Acipenser transmontanus) by Fourier transform infrared spectroscopy: a potential tool for caviar production management.

    Science.gov (United States)

    Lu, Xiaonan; Webb, Molly; Talbott, Mariah; Van Eenennaam, Joel; Palumbo, Amanda; Linares-Casenave, Javier; Doroshov, Serge; Struffenegger, Peter; Rasco, Barbara

    2010-04-14

    Fourier transform infrared spectroscopy (FT-IR, 4000-400 cm(-1)) was applied to blood plasma of farmed white sturgeon (N = 40) to differentiate and predict the stages of ovarian maturity. Spectral features of sex steroids (approximately 3000 cm(-1)) and vitellogenin (approximately 1080 cm(-1)) were identified. Clear segregation of maturity stages (previtellogenesis, vitellogenesis, postvitellogenesis, and follicular atresia) was achieved using principal component analysis (PCA). Progression of oocyte development in the late phase of vitellogenesis was also monitored using PCA based on changes in plasma concentrations of sex steroid and lipid content. The observed oocyte polarization index (PI, a measure of nuclear migration) was correlated with changes in plasma sex steroid levels revealed by FT-IR PCA results. A partial least squares (PLS) model predicted PI values within the range 0.12-0.40 (R = 0.95, SEP = 2.18%) from differences in spectral features. These results suggest that FT-IR may be a good tool for assessing ovarian maturity in farmed sturgeon and will reduce the need for the invasive ovarian biopsy required for PI determination. PMID:20170152

  10. EXPRESSED SEQUENCE TAG ANALYSIS OF A 4 YEAR-OLD CHINESE STURGEON PITUITARY%4龄中华鲟垂体的EST分析

    Institute of Scientific and Technical Information of China (English)

    曹宏; 周莉; 桂建芳

    2009-01-01

    Expressed sequence tag (EST) analysis is an efficient tool for gene discovery and for profiling gene expression. In order to isolate specific functional genes involved in reproduction and endocrine regulation and to reveal their evolutionary mechanisms in Chinese sturgeon (Acipenser sinensis Gray) , a chondrostean fish with a history of 140 million years, we constructed its pituitary cDNA library from a 4 year-old male. A total of 944 random clones were sequenced and compared with sequences in GenBank database. Among all the 944 EST clones, 802 (84.96%) clones were identified as 461 known genes, and additional 142 (15.04%) as unknown genes. Functional categorization indicated that the most abundantly expressed functional gene was the proopiomelanocortin (POMC), which accounted for almost 10. 17% of the overall expression, indicating its important function in the pituitary. Interestingly, the expression patterns of 7 unknown genes were analyzed in various tissues, such as heart, liver, spleen, kidney, muscle, testes, ovary and pituitary. Three different categories of expression patterns were observed from them. Several unknown ESTs, such as EG009334, EG009337, EG009338 and EG009340, were detected to be pituitary-specific, or pituitary and ovary-specific genes. Further studies on their functions will be very useful for better understanding the mechanisms of sturgeon reproduction biology and endocrinology.%表达序列标签(Expressed sequence tag,EST)是鉴定基因表达规律和发现新基因的一种有效的分子生物学手段.为了能在中华鲟(Acipenser sinensis Gray)中发现与生长和生殖内分泌调控相关的基因,我们构建了中华鲟垂体的SMART cDNA质粒文库.垂体是调节生长和生殖内分泌的重要器官.在本研究中,通过测序筛选得到了944个EST克隆,将所得EST与GenBank数据库中的序列进行比对,结果表明,802(84.96%)个克隆可以找到同源序列,共代表461个基因,其中含132

  11. REFERENCED TECHNOLOGICAL PERFORMANCES FOR STURGEON FINGERLING BREEDING IN INTENSIVE SYSTEM

    Directory of Open Access Journals (Sweden)

    MARILENA TALPES

    2013-12-01

    Full Text Available Work objective is to present an oversight regarding the modality to rearing the descendents of anadromous sturgeon species, Acipenser stellatus, Acipenser gueldenstaedti and Huso huso, obtained trough artificial reproduction, indicating successively, the production system, its management, alimentation strategy for sturgeon species and technological performances registered by these. Experiments developed during two phases, respectively the post-embryonary one and sapling rearing during of a 168 days period. Registered performances of sturgeon species material were assessed in conformity with specifically biotechnological indicators.

  12. The effects of time of reproductive migration on the biochemical composition and mobility trait of sperm in Persian sturgeon (Acipenser persicus brood stocks

    Directory of Open Access Journals (Sweden)

    Milad Kabir

    2011-07-01

    Full Text Available In this study the effects of reproductive migration of Persian sturgeon on some mobility traitsof sperm (duration of sperm movement and the percentage of motile sperms and on biochemicalcompounds (e.g. calcium, magnesium, glucose, total protein, sodium and potassium of semen wasinvestigated. For this purpose, time of reproductive migration in these brood stocks were divided to threeperiods (i.e. treatment 1 = March, treatment 2 = April and treatment 3 = May and in each period wesampled from those male fishes which had approximately the same sizes. The results showed that theduration of sperm movement (110.00±28.28, 327.85±85.38 and 55.00±7.07, the percentage of motilesperms (70.50±2.12, 88.60±8.38 and 66.00±0.00 and amounts of glucose (6.25±2.78, 1.18±0.44 and7.78±1.25, total protein (0.19±0.05, 0.41±0.10 and 0.07±0.00, potassium (3.57±1.31, 2.57±0.77 and4.46±0.14 and calcium (2.19±0.31, 2.59±0.81 and 11.99±6.35 of these fishes were significantlydifferent at different times of the reproductive migration (P0.05. Generally speaking, male brood stocks of Persian sturgeons in terms ofmentioned parameters are more appropriate in April compare to March and May months.

  13. Oceanic Distribution, Behaviour, and a Winter Aggregation Area of Adult Atlantic Sturgeon, Acipenser oxyrinchus oxyrinchus, in the Bay of Fundy, Canada.

    Directory of Open Access Journals (Sweden)

    Andrew Douglas Taylor

    Full Text Available Seasonal distribution of adult Atlantic sturgeon was examined using pop-up satellite archival tags (PSATs and ultrasonic transmitters deployed in the Saint John River, New Brunswick, Canada. Seven MK10 PSATs programmed for release in June 2012 and seven MiniPAT PSATs programmed for release in February and April 2013 were deployed in August 2011 and 2012, respectively. Eleven of 14 PSATs surfaced and transmitted depth and temperature data archived for the duration of their deployment (121-302 days. Among these eleven PSATs, five were recovered and 15-sec archival data was downloaded. Following exit from the Saint John River in the fall, tagged fish occupied a mean monthly depth of 76.3-81.6 m at temperatures as low as 4.9˚C throughout the winter before returning to shallower areas in the spring. The majority of ultrasonic detections occurred in the Bay of Fundy, but fish were detected as far as Riviere Saint-Jean, Quebec, approximately 1500 km from the Bay of Fundy (representing long-distance migratory rates of up to 44 km/day. All PSATs were first detected in the Bay of Fundy. Tags that released in February and April were found 5-21 km offshore of the Saint John Harbour, while tags that released in June were first detected in near shore areas throughout the Bay of Fundy. The substrate at winter tag release locations (estimated from backward numerical particle-tracking experiments consisted primarily of moraines and postglacial mud substrate with low backscatter strength, indicative of soft or smooth seabed. Based on the proximity of winter tag release locations, the consistent depths observed between fish, and previous research, it is suspected that a winter aggregation exists in the Bay of Fundy. This study expands the understanding of the marine distribution and range of Atlantic sturgeon on the east coast of Canada.

  14. The effects of different times of reproductive migration on biochemical compounds of ovarian fluid and on fertilization rate of Persian sturgeon (Acipenser persicus brood stocks

    Directory of Open Access Journals (Sweden)

    Milad Kabir

    2011-07-01

    Full Text Available In this study we investigated the effects of different times of reproductive migration onbiochemical compounds fluctuation (calcium, magnesium, glucose, cholesterol, total protein, sodium andpotassium and on the percentage of haematocrit of coelomic fluid and on the fertilization rate of 14Persian sturgeon brood stocks, during the spawning season (in March of 2008 until May of 2008. Forthis purpose, the period of sampling was divided to three groups, beginning (March, middle (April andend (May of spawning season of these fishes. The average of fork length and total weight of fishesbefore extraction of eggs of Persian sturgeon brood stocks in treatments 1, 2 and 3 were 166.5 ± 6.36,156.71 ± 8.69 and 174.5 ± 2.12 cm, also 33.0 ± 1.41, 30.1 ± 7.19 and 34.5 ± 0.70 kg, respectively. Onthe other hand, the average of percentage of fertilization rate in treatments 1, 2 and 3 were respectively166.5 ± 6.36, 156.71 ± 8.69 and 174.5 ± 2.12 cm, 33.0 ± 1.41, 30.1 ± 7.19 and 34.5 ± 0.70 kg , 67.5± 6.36, 68.3 ± 17.86 and 49.5 ± 3.53 percent. Our results showed that there were significantdifferences in calcium (5.41±0.51 - 1.65±0.03 and 11.99±1.41 mg dL-1, glucose (37.99±15.10 -23.61±6.45 and 11.3±3.26 mg dL-1, cholesterol (15.55±3.79 - 8.26±2.25 and 10.33±1.88 mg dL-1and sodium (185.94±38.86 - 103.98±31.78 and 180.75±6.47 mM L-1 between these treatments(P0.05. Overall, significant and insignificantfluctuations in coelomic fluid in spawning season of these fishes demonstrated that ovarian fluid intreatments number 1 (March and 3 (May had better characteristics for sperm mobility compared totreatments 2 (April.

  15. Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii%西伯利亚鲟Tbx3基因的克隆和表达分析

    Institute of Scientific and Technical Information of China (English)

    张昊; 范纯新; 宋佳坤

    2012-01-01

    Tbx3, a member of the TBX2 subfamily of T-box gene family, encodes a transcription factor with a highly conserved DNA-binding domain, which called T-domain. Tbx3 is involved in morphogenesis and organogenesis in vertebrates, such as limb development, heart remodeling, and neural placode differentiation. In the present study, a full-length 2 908 bp Tbx3 cDNA from Acipenser baerii (AbTbx3) was obtained using RT-PCR and RACE technique, which includes a 2 166 bp complete open reading frame encoding a putative peptide of 721 amino acids. AbTbx3 shares 73.5% identity with its human homolog. Particularly, the DNA-binding domain of AbTbx3 shared 95.2% identity with human Tbx3. Phylogenetic analysis revealed that AbTbx3 was grouped with Tbx3s in other vertebrates, which were clustered with Tbx2s and separated from Tbx4/5s. The predicted secondary and three-dimensional structures of the T-domain of AbTbx3 were remarkably similar to human Tbx3. Through semi-quantity RT-PCR, the expression of AbTbx3 was first detected at blastula stage during Siberian sturgeon embryonic development, increased gradually, reached its peak at early tail-bud stage and then decreased slightly. In adult sturgeon, AbTbx3 was strongly expressed in eye, brain, gill, intestines, pectoral fin and pelvic fin, but not in liver, blood, heart, kidney and muscle. The whole mount in situ hybridization showed that AbTbx3 was mainly expressed in the otic vesicle, hindbrain, dorsal notochord, pineal organ and dorsal fin bud in the larvae of stage 37 and 43.%Tbx3基因是一类重要的转录因子,在形态发生和器官形成中发挥着重要作用.该文克隆了西伯利亚鲟Tbx3基因(AbTbx3)cDNA的全长序列,该cDNA全长2 908 bp,包含一个2 166 bp的开放阅读框,编码721个氨基酸的多肽.分析表明:AbTbx3和人Tbx3的T-box结构域蛋白序列同源性达到95.2%,三维结构也具有高度的相似性.系统进化分析表明:AbTbx3与其他物种的Tbx3聚为一支,并在一个大的

  16. Effect of Water Velocity on Behavior,Antioxidant Enzyme and Residues of Enrofloxacin-HCl in Siberian Sturgeon(Acipenser baerii)%流速对西伯利亚鲟(Acipenser baerii)行为特性,抗氧化酶及体内盐酸恩诺沙星残留的影响

    Institute of Scientific and Technical Information of China (English)

    宋波澜; 许忠能; 王占全

    2014-01-01

    以西伯利亚鲟幼鱼[体质量(82.1±4.9)g]为实验材料,设置0 BL/s对照组,1.0和2.0 BL/s (体长/秒)3种流速条件,于处理后1 d,15 d和30 d 取样,进行鱼类行为学指标和抗氧化酶的测定。在此基础上,以50 mg/kg剂量,连续7 d混饲给药,研究了流速对停药后西伯利亚鲟肌肉组织中盐酸恩诺沙星残留的影响。结果表明:和静水对照组相比,实验期间各时段2个流速组西伯利亚鲟摆尾频率和趋流率均显著上升( P<0.05)。1.0 BL/s组流速下有最高的趋流率,2.0 BL/s组流速下有最高的摆尾频率;同一流速下西伯利亚鲟摆尾频率和趋流率随养殖时间的延长没有明显差异。 CAT、SOD和MDA 活力随着流速的增加而显著增强,两流速组均显著大于静水组( P<0.05),2.0 BL/s组流速下活力最高;3种抗氧化酶活力随养殖时间的延长差异均不显著。与静水对照组相比,停药后各时间点2.0 BL/s流速组鱼肌肉盐酸恩诺沙星含量均显著减少( P<0.05),在停药第8天已低于检出限,1.0 BL/s流速组鱼在停药第12天已低于检出限,而静水对照组则在停药20 d才低于检出限。研究结果说明适当的水流刺激可以诱导鱼类进行逆流运动,促进鱼体抗氧化酶活力的激活和加速药物残留的排除。%The influence of water velocity (0,1.0 and 2.0 BL/s),on behavior,antioxidant enzyme and residues of enrofloxacin in Siberian Sturgeon Acipenser baerii[weight (82.1±4.9)g] was studied.The experiments were divided into 1 d,15 d and 30 d in three stages.On this basis experiments were conducted to study the res-idues and depletion of enrofloxacin-HCl of Acipenser baerii after different water velocity treatments ( 0,1.0 and 2.0 BL/s) .Muscle was sampled after oral administration at the dose of 50 mg/kg for seven consecutive days.It was found that the rheotaxis frequency ( RF) and the tail beat

  17. Molecular cloning, characterization and tissue profiles of a HSP70 cDNA in Siberian sturgeon Acipenser baerii%西伯利亚鲟热休克蛋白HSP70 cDNA的克隆、序列分析和组织分布

    Institute of Scientific and Technical Information of China (English)

    田照辉; 徐绍刚; 王巍; 胡红霞; 董颖; 宋超

    2012-01-01

    A full length cDNA encoding heat shock protein 70 (HSP70) was cloned from liver RNA in Siberian sturgeon (Acipenser baerii) ,and was found to contain 2 343 bp, including 5'untranslated region of 140 bp, 3'un-translated region of 256 bp and an open reading frame of 1 947 bp. The deduced 648 amino acids sequence with estimated molecular of 71 000 and theoretical isoelectric point of 5. 21 had high similarity with other vertebrate HSP70 genes in NCBI. Sequence analysis indicated that the HSP70 cDNA was consisted of three signature sequences IDL-GTTYS, IFDLGGGTFDVSrL and IVLVGGSTRIPKIQK belonging to HSP70 family. Phylogenetic tree revealed that Alligator mississippiensis, Xenopus laevis ,Ambystoma mexicanum and Sebrian Sturgeon showed a close genetic relationship. Using fluorescent real-time quantitative PCR.the HSP70 was expressed in the liver, gill, spleen, heart, intestine, muscle, gonad and brain of Siberian sturgeon, with the peak in the spleen and the minimum in the liver (P<0.05) , indicating that the HSP70 cDNA could be used in understanding of anti-stress mechanism in Siberian sturgeon and in genetic breeding improvement.%采用普通PCR和RACE技术克隆了西伯利亚鲟Acipenser baerii热休克蛋白HSP70 cDNA的全序列,该序列全长为2343 bp,其中5'非编码区为140 bp,3'非编码区为256 bp,可读编码框(ORF)为1947bp,编码为648个氨基酸.该氨基酸序列中含有HSP70家族的3个特征序列——IDLGTTYS、IFDLGGGTFDVSIL和ⅣLVGGSTRIPKIQK,细胞质特征性保守序列为EEVD,C端重复序列为GGMP.该cDNA序列与其它生物的HSP70 cDNA序列一样具有很高的相似性.系统发育树显示,西伯利亚鲟与非洲爪蛙蟾Xenopus laevis、密西西比短吻鳄Alligator mississippiensis、美西螈Ambystoma mexicanum的亲缘关系较近.实时定量分析结果表明,水温为17.5℃时,西伯利亚鲟肝脏、鳃、脾脏、心脏、肌肉、中肠、性腺、脑8种组织中均有HSP70表达,其中HSP70在脾脏中的表达

  18. Adaptive alterations on gill Na(+), K(+)-ATPase activity and mitochondrion-rich cells of juvenile Acipenser sinensis acclimated to brackish water.

    Science.gov (United States)

    Zhao, Feng; Wu, Beibei; Yang, Gang; Zhang, Tao; Zhuang, Ping

    2016-04-01

    Understanding the physiological changes and osmoregulatory strategy is critical for anadromous species to adapt to large changes between freshwater and marine environments. In this study, juvenile Chinese sturgeon (Acipenser sinensis) were acclimated for 2 months to freshwater (FW, c. 0 ‰) and brackish water (BW, 15 ‰). Blood was assessed for changes in osmolality and ions. Gill tissue was assayed for Na(+), K(+)-ATPase (NKA) activity and immunohistochemical analysis on mitochondria-rich cells (MRCs). Serum osmolality and ions concentrations (Na(+), Cl(-) and K(+)) examined, except K(+), increased significantly in those specimens adapted to BW. However, the variations were within the range of effective hyperosmotic adaptation. The specific activity of gill NKA of juveniles adapted to BW was significantly higher (c. 1.6 times) than that of fish adapted to FW. MRCs were mainly presented in the interlamellar region of the filament and at the base of the lamella in either FW- or BW-acclimated individuals. In BW, the number and size of MRCs on filaments greatly increased. However, there was no significant difference in the number and size of the MRCs at the lamella region. Results show that juvenile Chinese sturgeon keep osmotic homeostasis in hyperosmotic environments by increasing gill NKA activity and MRCs' size and number, which is similar to other sturgeons and euryhaline teleosts. PMID:26614501

  19. Tissue profiles and expression of immunoglobulin M heavy chain mRNA in normal and bacterial infected Siberian sturgeon Acipenser baerii%西伯利亚鲟免疫球蛋白M重链区mRNA的组织分布和细菌感染后的表达

    Institute of Scientific and Technical Information of China (English)

    田照辉; 徐绍刚; 胡红霞; 朱华

    2014-01-01

    根据GenBank登录的西伯利亚鲟Acipernser baerii免疫球蛋白M重链区mRNA ( IgHM mRNA)设计引物,进行SYBR green实时定量。结果表明:鲟的脑、鳃、性腺、心脏、后肠、肝脏、肌肉、脾脏8种组织中, IgHM mRNA在脾脏中表达最高,在肝脏和心脏中表达较少,在脑、鳃、性腺、后肠、肌肉5种组织中几乎不表达;在水温22.6~24.5℃时,按100 g体质量注射0.1 mL菌液,给体质量为(260.2±37.8) g的西伯利亚鲟注射浓度为2.0×107 CFU/mL的维氏气单胞菌Aeromonas veronii,注射后40 h鱼开始死亡,62 h后停止死亡,注射后直至62 h时,处理组脾脏IgHM mRNA表达量一直处于对照组水平,93 h时处理组脾脏IgHM mRNA显著升高(P<0.05),为93 h对照组含量的3.7倍,说明机体已经开始特异性免疫应答。%Tissue profiles and expression of immunoglobulin M heavy chain mRNA ( IgHM mRNA ) in normal and bacterial challenging Siberian sturgeon Acipenser baerii with body weight of (260. 2±37. 8) g were investigated by a primer designed based on Siberian sturgeon in GenBank,and by Sybrgreen real time PCR. The results showed that IgHM mRNA was expressed in abundance in spleen,a little amount of IgHM mRNA expression in liver and heart, and almost no IgHM mRNA expression in gill, hindgut, muscle, gonad and brain. The mortality was observed in the Siberian sturgeon with muscular injection of pathogenic bacterium Aeromonas veronii with density of 2. 0 ×107 CFU/mL at a dose of 0. 1 mL/100 g body weight at water temperature of 22. 6-24. 5℃ 40 h after injection. Sixty-two h after injection, however, no mortality was found in the Siberian sturgeon with IgHM mRNA expression in spleen at a level as that in the fish in the control group. The Siberian sturgeon challenged for 93 h showed as higher 3. 7 times IgHM mRNA expression in spleen as that in the fish in the control group, indicating that the challenged Siberian sturgeon had activated specific immune response.

  20. Bioaccumulation of Cd, Pb and Zn in the edible and inedible tissues of three sturgeon species in the Iranian coastline of the Caspian Sea.

    Science.gov (United States)

    Mashroofeh, Abdulreza; Bakhtiari, Alireza Riyahi; Pourkazemi, Mohammad; Rasouli, Sana

    2013-01-01

    The accumulations of Cd, Pb and Zn were determined in edible and inedible tissues of Persian sturgeon (Acipenser persicus; n=27), stellate sturgeon (Acipenser stellatus; n=5) and beluga (Huso huso; n=4) collected from coastal waters of the South Caspian Sea from March to April 2011. Concentrations of metals evaluated in the caviar, muscle, liver, kidney, gills, ovary and heart of the three species of sturgeons have been assessed using the flame and graphite furnace atomic absorption spectrophotometer. Concentrations of Zn, Pb and Cd in the edible and inedible tissues were apparently different among the three species of sturgeons. Especially, beluga heart showed the highest concentrations of Zn and Pb and Cd in Persian sturgeon liver. The analyzed metals were found in the caviar and muscle samples of Persian sturgeon and likewise muscle samples two other sturgeon species at mean concentrations under the permissible limits proposed by MAFF (2000). PMID:22990022

  1. Characterization of serum immunoglobulins in a chondrostean fish, Acipenser baeri.

    Science.gov (United States)

    Partula, S; Charlemagne, J

    1993-01-01

    The euglobulin fraction of sturgeon (Acipenser baeri) serum was analyzed using electrophoretic and immunoblotting techniques. The major protein of this fraction is an IgM-like molecule composed of equimolar 70-kDa glycosylated H chains and 26-30 kDa L chains. In the absence of a reducing agent, the L and H polypeptides may form (mu 2L2)n high molecular weight polymers, mu 2L2 170-kDa units or L2 dimers. These different bonding patterns suggest some structural heterogeneity in the distribution of cysteine residues along the sturgeon Ig chains. The H chain N-terminal sequence indicates significant homologies with the conserved VHIII subgroup. Heavy chains antigenically different from the 70-kDa H chain were not detected, suggesting that IgM is the only Ig class synthesized by this sturgeon species. PMID:8299850

  2. Capture of white sturgeon larvae downstream of The Dalles Dam, Columbia River, Oregon and Washington, 2012

    Science.gov (United States)

    Parsley, Michael J.; Kofoot, Eric

    2013-01-01

    Wild-spawned white sturgeon (Acipenser transmontanus) larvae captured and reared in aquaculture facilities and subsequently released, are increasingly being used in sturgeon restoration programs in the Columbia River Basin. A reconnaissance study was conducted to determine where to deploy nets to capture white sturgeon larvae downstream of a known white sturgeon spawning area. As a result of the study, 103 white sturgeon larvae and 5 newly hatched free-swimming embryos were captured at 3 of 5 reconnaissance netting sites. The netting, conducted downstream of The Dalles Dam on the Columbia River during June 25–29, 2012, provided information for potentially implementing full-scale collection efforts of large numbers of larvae for rearing in aquaculture facilities and for subsequent release at a larger size in white sturgeon restoration programs.

  3. Kootenai River White Sturgeon Investigations; White Sturgeon Spawning and Recruitment Evaluation, 2003-2004 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Rust, Pete; Wakkinen, Virginia (Idaho Department of Fish and Game, Boise, ID)

    2005-06-01

    The objective of this research was to determine the environmental requirements for successful spawning and recruitment of the Kootenai River white sturgeon Acipenser transmontanus population. Annual tasks include monitoring and evaluating the various life stages of Kootenai River white sturgeon. Sampling for adult Kootenai River white sturgeon in 2003 began in March and continued through April. Eighty-one adult white sturgeon were captured with 3,576 hours of angling and set-lining effort in the Kootenai River. Discharge from Libby Dam and river stage at Bonners Ferry in 2003 peaked in May and early June. Flows remained above 500 m{sup 3}/s throughout June, decreased rapidly through mid July, and increased back to near 500 m{sup 3}/s after mid July and through mid August. By late August, flows had decreased to below 400 m{sup 3}/s. We monitored the movements of 24 adult sturgeon in Kootenay Lake, British Columbia (BC) and the Kootenai River from March 15, 2003 to August 31, 2003. Some of the fish were radio or sonic tagged in previous years. Twelve adult white sturgeon were moved upstream to the Hemlock Bar reach (rkm 260.0) and released as part of the Set and Jet Program. Transmitters were attached to seven of these fish, and their movements were monitored from the time of release until they moved downstream of Bonners Ferry. Eight additional radio-tagged white sturgeon adults were located in the traditional spawning reach (rkm 228-240) during May and June. Sampling with artificial substrate mats began May 21, 2003 and ended June 30, 2003. We sampled 717 mat d (a mat d is one 24 h set) during white sturgeon spawning. Three white sturgeon eggs were collected near Shortys Island on June 3, 2003, and five eggs were collected from the Hemlock Bar reach on June 5, 2003. Prejuvenile sampling began June 17, 2003 and continued until July 31, 2003. Sampling occurred primarily at Ambush Rock (rkm 244.0) in an attempt to document any recruitment that might have occurred from

  4. Green sturgeon physical habitat use in the coastal Pacific Ocean.

    Directory of Open Access Journals (Sweden)

    David D Huff

    Full Text Available The green sturgeon (Acipenser medirostris is a highly migratory, oceanic, anadromous species with a complex life history that makes it vulnerable to species-wide threats in both freshwater and at sea. Green sturgeon population declines have preceded legal protection and curtailment of activities in marine environments deemed to increase its extinction risk. Yet, its marine habitat is poorly understood. We built a statistical model to characterize green sturgeon marine habitat using data from a coastal tracking array located along the Siletz Reef near Newport, Oregon, USA that recorded the passage of 37 acoustically tagged green sturgeon. We classified seafloor physical habitat features with high-resolution bathymetric and backscatter data. We then described the distribution of habitat components and their relationship to green sturgeon presence using ordination and subsequently used generalized linear model selection to identify important habitat components. Finally, we summarized depth and temperature recordings from seven green sturgeon present off the Oregon coast that were fitted with pop-off archival geolocation tags. Our analyses indicated that green sturgeon, on average, spent a longer duration in areas with high seafloor complexity, especially where a greater proportion of the substrate consists of boulders. Green sturgeon in marine habitats are primarily found at depths of 20-60 meters and from 9.5-16.0°C. Many sturgeon in this study were likely migrating in a northward direction, moving deeper, and may have been using complex seafloor habitat because it coincides with the distribution of benthic prey taxa or provides refuge from predators. Identifying important green sturgeon marine habitat is an essential step towards accurately defining the conditions that are necessary for its survival and will eventually yield range-wide, spatially explicit predictions of green sturgeon distribution.

  5. Assessement of Growth Performances of Juvenile Sterlet Acipenser ruthenus Raised in a Recirculating Aquaculture System (RAS)

    OpenAIRE

    Mărioara Nicula; Zoltan-Tibor Szelei; Nicolae Pacala; Marian Bura; Eliza Simiz; Silvia Erina; Marian Hotea; Adela Marcu

    2015-01-01

    The study was conducted within the recirculating aquaculture system for sturgeon rearing in Herneacova village, Timis County, on a population of juvenile sterlet (Acipenser ruthenus) aged between 346-431 days. Specimens were housed in five round tanks (Ø=3 m, V=6 m3, h=100 cm) and fed with commercial extruded pellets (3 mm granulation, 48% CP, and 10% EE). Fish from each tank were individually weighed and measured once every two weeks. Bioproductive indices such body weight gain (BW), specifi...

  6. Migration and habitats of diadromous Danube River sturgeons in Romania: 1998-2000

    Science.gov (United States)

    Kynard, B.; Suciu, R.; Horgan, M.

    2002-01-01

    Upstream migrant adults of stellate sturgeon, Acipenser stellatus (10 in 1998, 43 in 1999) and Russian sturgeon, A. gueldenstaedtii (three in 1999) were captured at river km (rkm) 58-137, mostly in the spring, and tagged with acoustic tags offering a reward for return. The overharvest was revealed by tag returns (38% in 1998, 28% in 1999) and by harvest within 26 days (and before reaching spawning grounds) of the six stellate sturgeon tracked upstream. A drop-back of > 50% of the tagged sturgeon, some to the Black Sea, shows a high sensitivity to interruption of migration and capture/handling/holding. Harvesting and dropback prevented tracking of sturgeon to spawning sites. Gillnetting and tracking of stellate sturgeon showed that the autumn migration ended in early October (river temperature 16??C) and identified a likely wintering area at river km (rkm) 75-76 (St George Branch). Thus, fishery harvesting after early October captures wintering fish, not migrants. Rare shoreline cliffs in the lower river likely create the only rocky habitat for sturgeon spawning. A survey for potential spawning habitats found five sites with rocky substrate and moderate water velocity, all ???rkm 258. Drift netting caught early life-stages of 17 fish species and one sturgeon, a beluga, Huso huso, larva likely spawned at ???rkm 258. All diadromous Danube sturgeons likely spawn at ???rkm 258.

  7. Kootenai River White Sturgeon Investigations; White Sturgeon Spawning and Recruitment Evaluation, 2004-2005 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Rust, Pete; Wakkinen, Virginia (Idaho Department of Fish and Game, Boise, ID)

    2006-05-01

    The objective of this research was to determine the environmental requirements for successful spawning and recruitment of the Kootenai River white sturgeon Acipenser transmontanus population. Annual tasks include monitoring and evaluating the response of various life stages of Kootenai River white sturgeon to mitigation flows supplied by the United States Army Corps of Engineers (USACE). Sampling for adult Kootenai River white sturgeon in 2004 began in March and continued into May. One hundred forty-two adult white sturgeon were captured with 4,146 hours of angling and set-lining effort in the Kootenai River. Kootenai River discharge and stage at Bonners Ferry in 2004 peaked in mid December. Discharge remained below 400 cubic meters per second (cms) until June 1; then, because of a systems operations request (SOR), increased and remained between 480 and 540 cms through the end of June. From July through September, discharge ranged from 360 to 420 cms, decreasing to 168 cms by the end of October. Discharge increased again to above 625 cms by November 4 to increase winter storage in Lake Koocanusa and ranged from 310 to 925 cms through the end of December. We monitored the movements of 31 adult sturgeon in Kootenay Lake, British Columbia (BC) and the Kootenai River from mid-March until late August 2004. All telemetered fish were dual tagged with external sonic and radio transmitters, and some of the fish were tagged in previous years. Eighteen of the 31 telemetered adult white sturgeon were released at Hemlock Bar reach (rkm 260.0) as part of a research project to test the feasibility of moving sexually mature adult white sturgeon to areas with habitat types thought to be more suitable for successful egg hatching and early life stage recruitment. Marked fish were monitored from the time of release until they moved downstream of Bonners Ferry. Sampling for white sturgeon eggs with artificial substrate mats began May 3 and ended June 10, 2004. We sampled 650 mat days

  8. Kootenai River White Sturgeon Investigations; White Sturgeon Spawning and Recruitment Evaluation, 1999 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Paragamian, Vaughn L.; Kruse, Gretchen L.; Wakkinen, Virginia

    2001-11-01

    Sampling for adult Kootenai River white sturgeon Acipenser transmontanus began in March and continued through April 1999. Forty-six adult sturgeon were captured with 4,091 hours of angling and set-lining effort, while an additional three adult sturgeon were captured during gillnetting for juveniles. Flows for Kootenai River white sturgeon spawning were expected to be high because the snow pack in the basin was estimated at 130% of normal, but runoff came very slowly. Discharge from Libby Dam from mid-March through mid-June was maintained at 113 m{sup 3}/s (4,000 cfs). Flows in the Kootenai River at Bonners Ferry during early April, including local inflow, were 227-255 m{sup 3}/s (8,000-9,000 cfs) but increased gradually in late April to a peak of 657 m{sup 3}/s (23,200 cfs). Flows subsided in early May to about 340 m{sup 3}/s (12,000 cfs), but rose to 1,031 m{sup 3}/s (36,370 cfs) by Mary 26 because of local runoff, and white sturgeon began spawning. However, flows subsided again to 373 m{sup 3}/s (13,200 cfs) June 11, 1999 and some female white sturgeon with transmitters began leaving the spawning reach. Water temperature ranged from about 8 C to 10 C (45 F to 50 F) during these two weeks. On June 13 (two weeks after sturgeon began spawning), spawning and incubation flows from Libby Dam began. The flow was brought up to 1,136 m{sup 3}/s (40,100 cfs) and temperature rose to about 11 C (52 F). They sampled for 3,387 mat days (one mat day is a single 24 h set) with artificial substrate mats and captured 184 white sturgeon eggs. The Middle Shorty's Island reach (river kilometer [rkm] 229.6-231.5) produced the most eggs (144), with 388 mat days of effort; the Refuge section (rkm 234.8 to 237.5) with 616 mat days of effort produced 23 eggs; and the Lower Shorty's section produced 19 eggs with 548 days of mat effort. No eggs were collected above the Refuge section (> rkm 240.5) with 988 mat days of effort. They do not believe flows for sturgeon spawning in 1999

  9. The effects of Amax yeast fed to Persian sturgeon (Acipencer persicus) larvae via bioenrichment of Daphnia magna

    OpenAIRE

    Mohammad Lashkar Boloki; Hojatollah Jafaryan; Moein Faramarzi; Hosein Adineh

    2011-01-01

    This study was carried out to evaluate the effect of the product of commercial live bakers’ yeast ( Saccharomyces cerevisiae ) so called Amax on the growth and survival of Persian sturgeon( Acipenser persicus ) larvae via enrichment of Daphnia magna . The blends of Amax were used in three concentrations of 50, 100, 150 mg L-1 with Daphnia in suspension of broth. Every day Daphnia by one of concentrations was bioencapsulated for 10 hours and Persian sturgeon larvae were fed on it. The Aci...

  10. Sturgeon conservation genomics: SNP discovery and validation using RAD sequencing.

    Science.gov (United States)

    Ogden, R; Gharbi, K; Mugue, N; Martinsohn, J; Senn, H; Davey, J W; Pourkazemi, M; McEwing, R; Eland, C; Vidotto, M; Sergeev, A; Congiu, L

    2013-06-01

    Caviar-producing sturgeons belonging to the genus Acipenser are considered to be one of the most endangered species groups in the world. Continued overfishing in spite of increasing legislation, zero catch quotas and extensive aquaculture production have led to the collapse of wild stocks across Europe and Asia. The evolutionary relationships among Adriatic, Russian, Persian and Siberian sturgeons are complex because of past introgression events and remain poorly understood. Conservation management, traceability and enforcement suffer a lack of appropriate DNA markers for the genetic identification of sturgeon at the species, population and individual level. This study employed RAD sequencing to discover and characterize single nucleotide polymorphism (SNP) DNA markers for use in sturgeon conservation in these four tetraploid species over three biological levels, using a single sequencing lane. Four population meta-samples and eight individual samples from one family were barcoded separately before sequencing. Analysis of 14.4 Gb of paired-end RAD data focused on the identification of SNPs in the paired-end contig, with subsequent in silico and empirical validation of candidate markers. Thousands of putatively informative markers were identified including, for the first time, SNPs that show population-wide differentiation between Russian and Persian sturgeons, representing an important advance in our ability to manage these cryptic species. The results highlight the challenges of genotyping-by-sequencing in polyploid taxa, while establishing the potential genetic resources for developing a new range of caviar traceability and enforcement tools. PMID:23473098

  11. A novel approach to surveying sturgeon using side-scan sonar and occupancy modeling

    Science.gov (United States)

    Flowers, H. Jared; Hightower, Joseph E.

    2013-01-01

    Technological advances represent opportunities to enhance and supplement traditional fisheries sampling approaches. One example with growing importance for fisheries research is hydroacoustic technologies such as side-scan sonar. Advantages of side-scan sonar over traditional techniques include the ability to sample large areas efficiently and the potential to survey fish without physical handling-important for species of conservation concern, such as endangered sturgeons. Our objectives were to design an efficient survey methodology for sampling Atlantic Sturgeon Acipenser oxyrinchus by using side-scan sonar and to developmethods for analyzing these data. In North Carolina and South Carolina, we surveyed six rivers thought to contain varying abundances of sturgeon by using a combination of side-scan sonar, telemetry, and video cameras (i.e., to sample jumping sturgeon). Lower reaches of each river near the saltwater-freshwater interface were surveyed on three occasions (generally successive days), and we used occupancy modeling to analyze these data.We were able to detect sturgeon in five of six rivers by using these methods. Side-scan sonar was effective in detecting sturgeon, with estimated gear-specific detection probabilities ranging from 0.2 to 0.5 and river-specific occupancy estimates (per 2-km river segment) ranging from 0.0 to 0.8. Future extensions of this occupancy modeling framework will involve the use of side-scan sonar data to assess sturgeon habitat and abundance in different river systems.

  12. Fertility of a spontaneous hexaploid male Siberian sturgeon, Acipenser baerii

    Czech Academy of Sciences Publication Activity Database

    Havelka, M.; Hulák, M.; Ráb, Petr; Rábová, Marie; Lieckfeldt, D.; Ludwig, A.; Rodina, M.; Gela, D.; Pšenička, M.; Bytyutskyy, D.; Flajšhans, M.

    2014-01-01

    Roč. 15, č. 5 (2014). ISSN 1471-2156 Grant ostatní: GA MŠk(CZ) EE2.3.30.0049; GA MŠk(CZ) ED2.1.00/01.0024; GA ČR(CZ) GA523/08/0824 Institutional support: RVO:67985904 Keywords : Acipenseridae * polyploidy determination * sperm quality Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.397, year: 2014

  13. EFFECTS OF ACUTE THERMAL STRESS ON HSP70 MRNA, PHYSIOLOGY AND NONSPECIFIC IMMUNITY IN SIBERIAN STURGEON (ACIPENSER BAERII)%急性热应激对西伯利亚鲟HSP70mRNA表达、血清皮质醇和非特异性免疫的影响

    Institute of Scientific and Technical Information of China (English)

    田照辉; 徐绍刚; 王巍; 胡红霞; 马国庆

    2013-01-01

    The effect of acute thermal stress on HSP1Q mRNA expression, physiology and nonspecific immunity were investigated in Siberian sturgeon. In the experiment, ten fish were transferred froml7.5℃ to 27.5℃ water, then data were measured at lh and 3h. Fish in 17.5℃ water were taken as Oh group. Respiratory rate increased from 80-90 breaths/min to 210 breaths/min, then decreased to 180 breaths/min after 0.5h. Among the three tissues including gill, spleen and brain, the HSP70 mRNA expression in gill rose most quickly after lh stress, and was about 1.63 fold compared with the Oh group (P<0.05), then remained this level to 3h; The HSP10 mRNA expression in spleen and brain had nearly no change in lh thermal stress, then rose quickly from lh to 3h in the two tissues, especially in brain at 3h rose most quickly to about 1.77 fold compared with the lh group (P<0.05). Compared with the Oh group, serum cortisol increased quickly and was 5.14 fold (PO.05) at lh, then decreased quickly to 2.1 fold at 3h. Spleen macrophage respiratory burst achieved the maximum (P<0.05) at lh, then reduced to the Oh group level. Serum complement C3 increased at lh and decreased significantly at 3h (P<0.05). Serum lysozyme activity firstly elevated then decreased but had no significant differences. Serum SOD activity decreased when thermal stress time prolonged, and achieved the minimum at 3h (P<0.05). Serum MDA content decreased when thermal stress time prolonged and had no significant difference. The results showed that lh acute thermal stress promoted the nonspecific immunity and tolerance, but 3h acute thermal stress decreased it.%为研究西伯利亚鲟(Acipenser baerii)对急性热应激的抗逆机理,将体质量为(155.47±19.50) g的鱼从17.5 ℃迅速转至27.5 ℃水中,在1h和3h取样测定HSP70 mRNA表达变化、血清皮质醇和非特异性免疫指标.结果显示:急性热应激时鳃、脾和脑的HSP70 mRNA表达量升高,具有组织特异性,热应激1h时

  14. [Discrimination of interspecific hybrids in natural populations of Amur sturgeon fishes using multilocus RAPD-PCR markers].

    Science.gov (United States)

    Chelomina, G N; Rozhkovan, K V; Ivanov, S A

    2008-01-01

    RAPD-PCR analysis of 46 individuals of sturgeons from Amur River has been carried out. Genetic status of Amur sturgeon Acipenser schrenckii Brandt, 1869 and kaluga Huso dauricus Georgi, 1775 native populations has been estimated. Genetic evidences of hybrid origin for two phenotypical hybrids were obtained; estimations of genetic distances between species and hybrids appeared to be at interspecific level. The exact test for differentiation of populations (Exact test) and multidimensional scaling (MDS) analysis were estimated to be the most effective for species and hybrid discrimination, respectively. According to data obtained populations of sturgeon fishes which inhabit Amur River maintained an essential level of genetic variability; the presence of hybrids is regarded as one of risk factors. Multilocus RAPD-PCR markers admit as the convenient and reliable tool for genetic monitoring of Amur River sturgeons to preserve their gene pool. PMID:19140442

  15. Sturgeon nucleo-cytoplasmic large DNA virus phylogeny and PCR tests.

    Science.gov (United States)

    Clouthier, Sharon C; VanWalleghem, Elissa; Anderson, Eric D

    2015-12-01

    Sturgeon epitheliotropic nucleo-cytoplasmic large DNA viruses (NCLDVs) can cause a lethal disease of the integumentary system. These viruses have not been assigned to any currently recognized family or genus. In this study, phylogenetic analyses using the major capsid protein (MCP) showed that the sturgeon NCLDVs formed a cohesive taxonomic group, could be identified to the species or possibly sub-species level and formed a distinct evolutionary lineage within the Megavirales. The genetic relatedness of the sturgeon virus MCP allowed design of 3 PCR diagnostic tests with analytical specificity (ASp) inclusive of this group of viruses. The conventional PCR test, C1, had broader ASp than the 2 quantitative PCR tests, Q1 and Q2, and was inclusive of the sturgeon viruses as well as some viruses belonging to the families Mimi-, Phycodna-, or Iridoviridae. Q2 had broader specificity than Q1 but both tests recognized the sturgeon NCLDVs and did not cross-react with co-localizing sturgeon herpesviruses. Analytical test performance characteristics evaluated for Q1 and Q2 revealed sensitive assays with observed 50% limits of detection between 3 and 6.25 plasmid copies and high intra- and inter-assay repeatability. Q1 was used to test for sturgeon viruses in endangered populations of lake sturgeon Acipenser fulvescens within the Winnipeg River or Nelson River drainage systems of Manitoba, Canada. Test results indicated that namao virus is endemic in the Nelson River water basin. These tests meet the analytical requirements for diagnostic testing in Canada and are useful tools for disease management in sturgeon conservation stocking programs in North America. PMID:26648102

  16. Species and hybrid identification of sturgeon caviar: a new molecular approach to detect illegal trade.

    Science.gov (United States)

    Boscari, E; Barmintseva, A; Pujolar, J M; Doukakis, P; Mugue, N; Congiu, L

    2014-05-01

    Overexploitation of wild populations due to the high economic value of caviar has driven sturgeons to near extinction. The high prices commanded by caviar on world markets have made it a magnet for illegal and fraudulent caviar trade, often involving low-value farmed caviar being sold as top-quality caviar. We present a new molecular approach for the identification of pure sturgeon species and hybrids that are among the most commercialized species in Europe and North America. Our test is based on the discovery of species-specific single nucleotide polymorphisms (SNPs) in the ribosomal protein S7, supplemented with the Vimentin gene and the mitochondrial D-loop. Test validations performed in 702 specimens of target and nontarget sturgeon species demonstrated a 100% identification success for Acipenser naccarii, A. fulvescens, A. stellatus, A. sinensis and A. transmontanus. In addition to species identification, our approach allows the identification of Bester and AL hybrids, two of the most economically important hybrids in the world, with 80% and 100% success, respectively. Moreover, the approach has the potential to identify many other existing sturgeon hybrids. The development of a standardized sturgeon identification tool will directly benefit trade law enforcement, providing the tools to monitor and regulate the legal trade of caviar and protect sturgeon stocks from illicit producers and traders, hence contributing to safeguarding this group of heavily threatened species. PMID:24219811

  17. Assessement of Growth Performances of Juvenile Sterlet Acipenser ruthenus Raised in a Recirculating Aquaculture System (RAS

    Directory of Open Access Journals (Sweden)

    Mărioara Nicula

    2015-10-01

    Full Text Available The study was conducted within the recirculating aquaculture system for sturgeon rearing in Herneacova village, Timis County, on a population of juvenile sterlet (Acipenser ruthenus aged between 346-431 days. Specimens were housed in five round tanks (Ø=3 m, V=6 m3, h=100 cm and fed with commercial extruded pellets (3 mm granulation, 48% CP, and 10% EE. Fish from each tank were individually weighed and measured once every two weeks. Bioproductive indices such body weight gain (BW, specific growth rate (SGR, and feed conversion ratio (FCR were calculated at the end of an 85 days experimental period. Statistical analysis was performed using the SPPS IBM 22 and Minitab 16 software. Data were reported as Mean±Sx at a significant level of p<0.05. Testing differences between means was realized by ANOVA completed with post-hoc Tukey test. The results obtained indicate that the growth performance of juvenile Acipenser ruthenus seems to be similar and even better than that of other sturgeons species reared in recirculating aquaculture system.

  18. 中华鲟初次全人工繁殖的特性研究%THE REPRODUCTIVE BIOLOGY OF FIRST FILIAL GENERATION OF ACIPENSER SINENSIS GROWING UP IN THE FRESHWATER ENVIRONMENT

    Institute of Scientific and Technical Information of China (English)

    郭柏福; 常剑波; 肖慧; 朱滨; 万建义; 田家元; 舒德斌

    2011-01-01

    Chinese sturgeon (Acipenser sinensis Gary) is an endangered anadromous species in the IUCN Red List. It was characterized as the national level key protected animal of China in 1988 and under the protection of CITES Appendix II in 1998. In order to realize the artificial propagation, mature Chinese sturgeons were captured below the Gezhouba Dam in the Yangtze River every year since 1982. And the induction of wild Chinese sturgeon succeeded in 1984. From then on, the artificially reproduced larvae of Chinese sturgeon were released every year. With the declining of the population, capture of wild Chinese sturgeon was prohibited by the government from 2008, so induction of cultured sturgeons was necessary to increase the quantity of larvae in the Yangtze River. From 1987 to 1997, many progenies of induced Chinese sturgeons captured from the Yangtze River were tried to culture in complete freshwater environment in Chinese sturgeon Institute. Twenty four growing-up individuals were selected for the attempts of the artificial propagation study in 2006. We successfully made the gonads of these individuals develop into mature in freshwater environment via controlling culture environment temperature, lowering temperature to induce and supplying particular nutrient in 2009. The gonads were monitored by the combination of ultrasound detection and manual puncture detection. Primary biological data of egg, such as egg diameter and egg weight, the migration of the egg polar nucleus, were checked to confirm whether the female were mature and the microscope was used to check whether the male were mature. There were totally eight individuals whose gonads reached Phase III in 2008 and 2009. We found two mature brood fish (No. 6B7A and 391D) suitable to conduct artificial propagation via checking gonads in September 2009, and then we tried to conduct the first artificial induction in 29 September 2009, and part of the semen were Cryopreservation. In 22 November 2009, we confirmed

  19. Sterilization of sterlet Acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene.

    Science.gov (United States)

    Linhartová, Zuzana; Saito, Taiju; Kašpar, Vojtěch; Rodina, Marek; Prášková, Eva; Hagihara, Seishi; Pšenička, Martin

    2015-10-15

    Sturgeons (chondrostean, acipenseridae) are ancient fish species, widely known for their caviar. Nowadays, most of them are critically endangered. The sterlet (Acipenser ruthenus) is a common Eurasian sturgeon species with a small body size and the fastest reproductive cycle among sturgeons. Such species can be used as a host for surrogate production; application is of value for recovery of critically endangered and huge sturgeon species with an extremely long reproductive cycle. One prerequisite for production of the donor's gametes only is to have a sterile host. Commonly used sterilization techniques in fishes such as triploidization or hybridization do not guarantee sterility in sturgeon. Alternatively, sterilization can be achieved by using a temporary germ cell exclusion-specific gene by a knockdown agent, the antisense morpholino oligonucleotide (MO). The targeted gene for the MO is the dead end gene (dnd) which is a vertebrate-specific gene encoding a RNA-binding protein which is crucial for migration and survival of primordial germ cells (PGCs). For this purpose, a dnd homologue of Russian sturgeon (Agdnd), resulting in the same sequence in the start codon region with isolated fragments of sterlet dnd (Ardnd), was used. Reverse transcription polymerase chain reaction confirmed tissue-specific expression of Ardnd only in the gonads of both sexes. Dnd-MO for depletion of PGCs together with fluorescein isothiocyanate (FITC)-biotin-dextran for PGCs labeling was injected into the vegetal region of one- to four-cell-stage sterlet embryos. In the control groups, only FITC was injected to validate the injection method and labeling of PGCs. After optimization of MO concentration together with volume injection, 250-μM MO was applied for sterilization of sturgeon embryos. Primordial germ cells were detected under a fluorescent stereomicroscope in the genital ridge of the FITC-labeled control group only, whereas no PGCs were present in the body cavities of morphants

  20. Green Sturgeon Acoustic Monitoring

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This database is used to hold tracking data for green sturgeon tagged in Central California. The data collection began in late 2002 and is ongoing.

  1. Organchlorine compounds in pallid sturgeon

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Pallid sturgeon, a federally endangered species, are extremely rare, and few specimens are available for study. We obtained 12 Mississippi River pallid sturgeon for...

  2. The effects of Amax yeast fed to Persian sturgeon (Acipencer persicus larvae via bioenrichment of Daphnia magna

    Directory of Open Access Journals (Sweden)

    Mohammad Lashkar Boloki

    2011-07-01

    Full Text Available This study was carried out to evaluate the effect of the product of commercial live bakers’ yeast ( Saccharomyces cerevisiae so called Amax on the growth and survival of Persian sturgeon( Acipenser persicus larvae via enrichment of Daphnia magna . The blends of Amax were used in three concentrations of 50, 100, 150 mg L-1 with Daphnia in suspension of broth. Every day Daphnia by one of concentrations was bioencapsulated for 10 hours and Persian sturgeon larvae were fed on it. The Acipenser persicus larvae were fed from Daphnia on the base of the 50 percent of their body weight five times a day. The control group was fed on not enriched Daphnia. The gained body weight in experimental treatments of sturgeon larvae had significant difference compared to control treatment (p0.05. The Amax had significant positive effects on survival rate in comparison with control treatment (p<0.05. The experiments indicated that the product of Saccharomyces cerevisiae has a high ability to increase the growth parameters and feeding efficiency in cultivation system of sturgeon fish.

  3. Short-term hydrodynamic features of Chinese sturgeon spawning ground under reservoir regulation conditions%水库调度影响下中华鲟产卵场的水动力特征

    Institute of Scientific and Technical Information of China (English)

    毛劲乔; 李智; 戴会超; 柯云

    2014-01-01

    三峡工程试验性蓄水于2010年成功蓄至175 m,开始逐渐进入正常运行期.在此背景下,分析近年来三峡-葛洲坝实际联合调度运行对坝下中华鲟产卵场水动力时空特征的影响,对加深理解中华鲟产卵适应性对水利工程调控的动态响应有重要意义.针对葛洲坝至宜昌江段的中华鲟产卵场建立了三维水动力模型,以出现2次鲟鱼产卵繁殖的2011年产卵日为代表性工况,开展了水利工程调度运行影响下的产卵场短期水动力特征模拟研究.研究表明:对鲟鱼自然繁殖起直接作用的河段底层流场较规则稳定,但在水流交汇处和地形突变处会出现局部紊乱变化;上、下产卵区的流速为0.8~1.4 m/s,在大江电厂与二江电厂下游均有流速较大的分布区,流速最大值出现在二江电厂泄水处下游;适宜中华鲟繁殖的流速范围出现在大江下游导流墙尾段至江心隔流堤前段1/3处与江心隔流堤尾端至下游约700 m内.%The water level of Three Gorges Reservoir (TGR)reached to 175 meters in 2010,indica-ting this hydropower project had started its normal operation.Chinese sturgeon spawning ground is lo-cated at downstream of Gezhouba reservoir immediately and the water level of the ground is affected by regulation of both reservoirs.Since hydrodynamics in the ground can play a crucial role in multiplica-tion of Chinese sturgeon,effects of regulation of both reservoirs on the hydrodynamic characteristics of Chinese sturgeon spawning ground are investigated to explore its suitability for reservoir regulation of a hydraulic project.A three-dimensional hydrodynamic model is built for the Chinese sturgeon spawning ground from Gezhouba to Yichang reach.The short-term hydrodynamic characteristics of the spawning ground are studied by simulation method at two water levels at which two spawning events were observed in 2011.It is shown that the bottom layer flow

  4. PHYSICO-CHEMICAL LIMITATIVE FACTORS FOR GROWTH AND DEVELOPMENT IN STERLET (ACIPENSER RUTHENUS LINNAEUS, 1758 IN EXTENSIVELY SYSTEM

    Directory of Open Access Journals (Sweden)

    R. C. DIMA

    2013-07-01

    Full Text Available The oldest and most common method of increasing fish is a fish breeding ponds in which the supervision of nutrition and growth of biological material. Sterlet (Acipenser ruthenus ruthenus Linnaeus 1785 is the 4th of sturgeon scale and economic importance as a share of production of these fish .Monitoring of physicochemical parameters of sturgeons ponds has a crucial role to obtain satisfactory yields both in qualitative and quantitative. Chemical characteristics of water were determined in laboratory ecosystems Chemistry of the Institute of Research and Development for Ecology Aquaculture, Fisheries and Aquaculture Galaţi for the samples have been taken of the total water. Determination of the chemical characteristics was performed by standardized methods. Physic-chemical parameters of water were determined according to norm on the classification of surface water quality in order to establish the ecological status of water (Order no. 161/2006, for Class II of quality.

  5. Experimentelle Untersuchungen an großen Juvenilen des Europäischen Störs, Acipenser sturio Linnaeus, 1758 unter Berücksichtigung ernährungs- und wachstumsspezifischer Aspekte.

    OpenAIRE

    Hensel, Emanuel Clemens Konrad

    2010-01-01

    The European sturgeon Acipenser sturio L., 1758 has nearly approached extinction in its original and overall distribution areas from the White and the Baltic Seas, to the Black Sea, including the North and Mediterranean Seas as well as the Eastern North Atlantic (HOLCIK et al., 1989). The species occurs regularly only in a recent population in the Gironde basin in France (ROCHARD et al., 1990; WILLIOT et al., 1997, KIRSCHBAUM et al., 2000). This population has still decreased dramatically sin...

  6. Identification of plasma glucocorticoids in pallid sturgeon in response to stress

    Science.gov (United States)

    Webb, M.A.H.; Allert, J.A.; Kappenman, K.M.; Marcos, J.; Feist, G.W.; Schreck, C.B.; Shackleton, C.H.

    2007-01-01

    Compared to teleosts, little is known about the stress response in chondrosteans, and the glucocorticoid(s) most responsive to stress have never been definitively determined in sturgeon. In terms of cortisol production, pallid sturgeon (Scaphirhynchus albus) have a low physiological response to stress compared to other sturgeons (Acipenser sp.). Because of this, our null hypothesis was that cortisol is not the predominant glucocorticoid secreted in response to stress in pallid sturgeon. Our objective was to identify the putative glucocorticoids present in the plasma of pallid sturgeon during the stress response. Pallid sturgeon were subjected to a severe confinement stress (12 h) with an additional handling stressor for the first 6 h. Control fish were not subjected to confinement but were handled only to collect blood. Blood plasma was collected at time 0, 6, and 12 h. Gas chromatography/mass spectrometry was used to screen the plasma for the spectrum of glucocorticoids and determine the putative steroid secreted during the stress response. Cortisol was the primary glucocorticoid detected in stressed pallid sturgeon. In addition, the cortisol metabolites cortisone, alloTHE (5??-pregnane-3??,17??,21-triol-11,20-dione), allo-??-cortolone (3??,17??,20??,21-tetrahydro-5??-pregnan-11-one), and allo-??-cortolone (3??,17??,20??,21-tetrahydro-5??-pregnan-11-one) were detected. Plasma cortisol increased from a resting concentration of 0.67 ng/ml to 10.66 ng/ml at 6 h followed by a decrease to 6.78 ng/ml by 12 h. Plasma glucose increased significantly by time 6 and 12 h in both stressed and unstressed groups and remained elevated at time 12 h, while resting lactate concentrations were low to non-detectable and did not increase significantly with the stressor over time. Cortisol was the primary glucocorticoid synthesized and secreted in response to a stressor in pallid sturgeon. Though the proportional increase in plasma cortisol in stressed pallid sturgeon was lower than

  7. Kootenai River white sturgeon investigations. Chapter 1: Kootenai River white sturgeon spawning and recruitment evaluation; Annual report, January 1--December 31, 1996

    International Nuclear Information System (INIS)

    Test flows for Kootenai River white sturgeon Acipenser transmontanus spawning, scheduled for June 1996, were postponed until July. However, an estimated 126% snow pack and unusually heavy precipitation created conditions for sturgeon spawning that were similar to those occurring before construction of Libby Dam. Discharge in the Kootenai River at Bonners Ferry rose to nearly 1,204 m3/s (42,500 cfs) during May and water temperature ranged from 5.8 C to 8.4 C (42 F to 47 F). Migration of adult white sturgeon into spawning areas occurred in late May during a rising hydrograph. Discharge and water temperature were rising and had reached approximately 1,077 m3/s (38,000 cfs) and 8 C (46 F). Discharge at Bonners Ferry peaked at about 1,397 m3/s (49,300 cfs) on June 5. A total of 348 eggs (and one egg shell) were collected with 106,787 h of mat effort during the flow events. The first white sturgeon eggs were collected on June 8 and continued through June 30. Staging of eggs and back-calculating to spawning dates indicated there were at least 18 spawning episodes between June 6 and June 25. Discharge on June 6 was 1,196 m3/s (42,200 cfs) and decreased steadily to 850 m3/s (30,000 cfs) by June 26. Although sturgeon spawned in the same reach of river that they had during 1994 and 1995, the majority of eggs were found significantly (P = 0.0001) farther upstream than 1994 and 1995 and this in turn may be related to elevation of Kootenay Lake

  8. Denaturation and intermediates study of two sturgeon hemoglobins by n-dodecyl trimethylammonium bromide

    Science.gov (United States)

    Ariaeenejad, Shohreh; Habibi-Rezaei, Mehran; Kavousi, Kaveh; Jamili, Shahla; Fatemi, Mohammad Reza; Hong, Jun; Poursasan, Najmeh; Sheibani, Nader; Moosavi-Movahedi, Ali. A.

    2013-01-01

    Varieties of hemoglobin (Hb) forms exist in fish, which are usually well adapted to the different ecological conditions or various habitats. In the current study, Hbs from two Sturgeon species of the Southern Caspian Sea Basin were purified and studied upon interaction with n-dodecyl trimethylammonium bromide (DTAB; as a cationic surfactant) by various methods including UV-visible absorption, dynamic light scattering (DLS), and ANS fluorescence spectrophotometry. The chemometric analysis of Hbs was investigated upon interaction with DTAB under titration, using UV-visible absorption spectra. The chemometric resolution techniques were used to determine the number of the components and mole fraction of the oxidized Hbs. These results provided the evidence for the existence of three different molecular components including native (N), intermediate (I) and denatured (D) in sturgeon Hbs. According to the distribution of intermediates, which were broadened in a range of DTAB concentration, the aggregation states, DLS experiments, and thermal stability (Tm obtained by differential scanning calorimetry (DSC)), the Acipenser stellatus Hb was more stable compared to Acipenser persicus Hb. These results demonstrate a significant relationship between the stability of fish Hbs and the habitat depth requirements. PMID:23142155

  9. Kootenai River White Sturgeon Investigations : White Sturgeon Spawning and Recruitment Evaluation, 1998 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Paragamian, Vaughn L.; Kruse, Gretchen L.; Wakkinen, Virginia

    2001-03-01

    Flows in the Kootenai River for white sturgeon Acipenser transmontanus spawning in 1998 were expected to be at a minimum because the snow pack in the basin was only about 79% normal, and local inflow was expected to be very low, <142 m{sup 3}/s (5,000 cfs). Flows in the Kootenai River at Bonners Ferry from late April through early May were at about 425 m{sup 3}/s (15,000 cfs) while water temperature ranged from about 8 to 10 C (45 to 50 F). Spawning and incubation flows from Libby Dam began on May 18 when flow at the dam was brought up to 765 m{sup 3}/s (27,000 cfs). Unusually frequent rains and several enormous storms brought peak flows at Bonners Ferry to over 1,175 m{sup 3}/s (41,500 cfs) on May 27, temperature ranged between 8 and 10.6 C (45 to 51 F). Flow gradually subsided at Bonners Ferry during June and was steady at 708 to 765 m{sup 3}/s (25,000 to 27,000 cfs) while temperature gradually rose to 14.4 C (58 F). Forty-seven adult white sturgeon were captured with 4,220 hours of angling and setlining effort between March 1 and April 15, 1998 by the Idaho Department of Fish and Game (IDFG). Sonic and radio tags were attached to four female and five male sturgeon during this effort. From April 1 through July 31, 1998, a total of 17 fish were monitored specifically for pre-spawn and spawning activities. White sturgeon spawning location, timing, frequency, and habitat were evaluated by sampling for eggs with artificial substrate mats. Four hundred and eighty-four eggs were collected, 393 eggs (81%) were collected on 60 standard mats, and 91 eggs (19%) were collected on seven experimental mats with drift nets. Ten eggs collected with experimental mats were found mixed with sand, suggesting eggs are moving in the lower water column with sand. The middle Shorty's Island reach (rkm 229.6-231.5) produced the most eggs (173) while the Deep Creek section (rkm 237.6-240.5) produced 112 eggs. No eggs were collected above the Deep Creek section (>rkm 240.5). Four

  10. Electronic tagging of green sturgeon reveals population structure and movement among estuaries

    Science.gov (United States)

    Lindley, S.T.; Erickson, D.L.; Moser, M.L.; Williams, G.; Langness, O.P.; McCovey, B.W., Jr.; Belchik, M.; Vogel, D.; Pinnix, W.; Kelly, J.T.; Heublein, J.C.; Klimley, A.P.

    2011-01-01

    Green sturgeon Acipenser medirostris spend much of their lives outside of their natal rivers, but the details of their migrations and habitat use are poorly known, which limits our understanding of how this species might be affected by human activities and habitat degradation.We tagged 355 green sturgeon with acoustic transmitters on their spawning grounds and in known nonspawning aggregation sites and examined their movement among these sites and other potentially important locations using automated data-logging hydrophones. We found that green sturgeon inhabit a number of estuarine and coastal sites over the summer, including the Columbia River estuary, Willapa Bay, Grays Harbor, and the estuaries of certain smaller rivers in Oregon, especially the Umpqua River estuary. Green sturgeon from different natal rivers exhibited different patterns of habitat use; most notably, San Francisco Bay was used only by Sacramento River fish, while the Umpqua River estuary was used mostly by fish from the Klamath and Rogue rivers. Earlier work, based on analysis of microsatellite markers, suggested that the Columbia River mixed stock was mainly composed of fish from the Sacramento River, but our results indicate that fish from the Rogue and Klamath River populations frequently use the Columbia River as well. We also found evidence for the existence of migratory contingentswithin spawning populations.Our findings have significant implications for the management of the threatened Sacramento River population of green sturgeon, which migrates to inland waters outside of California where anthropogenic impacts, including fisheries bycatch and water pollution, may be a concern. Our results also illustrate the utility of acoustic tracking to elucidate the migratory behavior of animals that are otherwise difficult to observe. ?? American Fisheries Society 2011.

  11. Adaption of egg and larvae sampling techniques for lake sturgeon and broadcast spawning fishes in a deep river

    Science.gov (United States)

    Roseman, Edward F.; Kennedy, Gregory W.; Craig, Jaquelyn; Boase, James; Soper, Karen

    2011-01-01

    In this report we describe how we adapted two techniques for sampling lake sturgeon (Acipenser fulvescens) and other fish early life history stages to meet our research needs in the Detroit River, a deep, flowing Great Lakes connecting channel. First, we developed a buoy-less method for sampling fish eggs and spawning activity using egg mats deployed on the river bottom. The buoy-less method allowed us to fish gear in areas frequented by boaters and recreational anglers, thus eliminating surface obstructions that interfered with recreational and boating activities. The buoy-less method also reduced gear loss due to drift when masses of floating aquatic vegetation would accumulate on buoys and lines, increasing the drag on the gear and pulling it downstream. Second, we adapted a D-frame drift net system formerly employed in shallow streams to assess larval lake sturgeon dispersal for use in the deeper (>8 m) Detroit River using an anchor and buoy system.

  12. Assigning sex and reproductive stage to adult Lake Sturgeon using ultrasonography and common morphological measurements

    Science.gov (United States)

    Chiotti, Justin A.; Boase, James C.; Hondorp, Darryl W.; Briggs, Andrew S.

    2016-01-01

    Sex determination of fish species is difficult to assess when sexual dimorphism and gametes are not apparent. For threatened and endangered fish species, noninvasive techniques are needed when determining sex to minimize stress and the potential for mortality. We evaluated the use of a portable ultrasound unit to determine sex of Lake Sturgeon Acipenser fulvescens in the field. Ultrasound images were collected from 9 yellow-egg (F2, F3), 32 black-egg (F4, F5), and 107 fully developed male (M2) Lake Sturgeon. Two readers accurately assigned sex to 88–96% of fish, but accuracy varied in relation to maturity stage. Black-egg females and fully developed males were correctly identified for 89–100% of the fish sampled, while these two readers identified yellow-egg females only 33% and 67% of the time. Time spent collecting images ranged between 2 and 3 min once the user was comfortable with operating procedures. Discriminant analysis revealed the total length : girth ratio was a strong predictor of sex and maturity, correctly classifying 81% of black-egg females and 97% of the fully developed males. However, yellow-egg females were incorrectly classified on all occasions. This study shows the utility of using ultrasonography and a total length : girth ratio for sex determination of Lake Sturgeon in later reproductive stages around the spawning season.

  13. Using a semi-natural stream to produce young sturgeons for conservation stocking: Maintaining natural selection during spawning and rearing

    Science.gov (United States)

    Kynard, B.; Pugh, D.; Parker, T.; Kieffer, M.

    2011-01-01

    Young sturgeons used for conservation stocking are presently produced using the same methods used for commercial culture. To determine if young sturgeons could be produced without relaxing natural selection factors, we developed a semi-natural stream where we annually studied mating of wild shortnose sturgeon (Acipenser brevirostrum) observed movement of gametes released freely during spawning, and estimated the number of larvae produced by various densities of spawned eggs. The stream had a bottom area of 18.8m2, a rubble-gravel bottom, and a mean bottom current at 0.6 depth during spawning of 48cms-1 (range, 17-126cms-1). Wild adults successfully spawned in the stream each year for 7years (2002-2008). Some females and males were more successful during spawning than others, suggesting an unequal fitness during spawning among wild individuals, which is different than the controlled spawning fitness of individuals in hatcheries. Male and female gametes spawned naturally must connect quickly in the fast current or fail, a selection factor absent in hatcheries. The number of larvae produced was inversely related to spawned egg densitym-2 (R2=0.65) and the maximum number of larvae produced was 8000-16000 (425-851larvaem-2 of bottom). Artificial spawning streams have the potential to contribute to sturgeon restoration. ?? 2011 Blackwell Verlag, Berlin.

  14. Influence of environmental related concentrations of heavy metals on motility parameters and antioxidant responses in sturgeon sperm.

    Science.gov (United States)

    Li, Zhi-Hua; Li, Ping; Dzyuba, Borys; Randak, Tomas

    2010-12-01

    The effects of heavy metals (Cd, Cr and Cd+Cr) on the motility parameters and oxidative stress of sterlet (Acipenser ruthenus) sperm were investigated in vitro. Sturgeon sperm were exposed for 2h to heavy metals at environmental related concentrations (0.1mgL(-1) Cr, 0.001mgL(-1) Cd, 0.1mgL(-1) Cr+0.001mgL(-1) Cd) and higher concentrations (5.0mgL(-1) Cr, 0.05mgL(-1) Cd, 5.0mgL(-1) Cr+0.05mgL(-1) Cd). Results revealed that environmental concentrations of heavy metals had no significant influence on motility parameters and antioxidant responses indices in sturgeon sperm, except for LPO level and SOD activity. But higher concentrations of these metals induced oxidative tress in sturgeon sperm in vitro, associated with sperm motility parameters inhibition. Our results suggest that using of sperm in vitro assays may provide a novel and efficiently means for evaluating the effects of residual heavy metals in aquatic environment on sturgeon. PMID:20836996

  15. Genetic effects of habitat restoration in the Laurentian Great Lakes: an assessment of lake sturgeon origin and genetic diversity

    Science.gov (United States)

    Jamie Marie Marranca; Amy Welsh; Roseman, Edward F.

    2015-01-01

    Lake sturgeon (Acipenser fulvescens) have experienced significant habitat loss, resulting in reduced population sizes. Three artificial reefs were built in the Huron-Erie corridor in the Great Lakes to replace lost spawning habitat. Genetic data were collected to determine the source and numbers of adult lake sturgeon spawning on the reefs and to determine if the founder effect resulted in reduced genetic diversity. DNA was extracted from larval tail clips and 12 microsatellite loci were amplified. Larval genotypes were then compared to 22 previously studied spawning lake sturgeon populations in the Great Lakes to determine the source of the parental population. The effective number of breeders (Nb) was calculated for each reef cohort. The larval genotypes were then compared to the source population to determine if there were any losses in genetic diversity that are indicative of the founder effect. The St. Clair and Detroit River adult populations were found to be the source parental population for the larvae collected on all three artificial reefs. There were large numbers of contributing adults relative to the number of sampled larvae. There was no significant difference between levels of genetic diversity in the source population and larval samples from the artificial reefs; however, there is some evidence for a genetic bottleneck in the reef populations likely due to the founder effect. Habitat restoration in the Huron-Erie corridor is likely resulting in increased habitat for the large lake sturgeon population in the system and in maintenance of the population's genetic diversity.

  16. Evaluate Potential Means of Rebuilding Sturgeon Populations in the Snake River between Lower Granite and Hells Canyon Dams, 2000 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Everett, Scott R.; Tuell, Michael A. (Nez Perce Tribe, Department of Fishereis Resource Management, Lapwai, ID)

    2003-03-01

    The specific research goal of this project is to identify means to restore and rebuild the Snake River white sturgeon (Acipenser transmontanus) population to support a sustainable annual subsistence harvest equivalent to 5 kg/ha/yr (CBFWA 1997). Based on data collected, a white sturgeon adaptive management plan will be developed. This 2000 annual report covers the fourth year of sampling of this multi-year study. In 2000 white sturgeon were captured, marked, and population data were collected in the Snake and Salmon rivers. The Snake River was sampled between Lower Granite Dam (rkm 174) and the mouth of the Salmon River (rkm 303), and the Salmon River was sampled from its mouth upstream to Hammer Creek (rkm 84). A total of 53,277 hours of setline effort and 630 hours of hook-and-line effort was employed in 2000. A total of 538 white sturgeon were captured and tagged in the Snake River and 25 in the Salmon River. Since 1997, 32.8 percent of the tagged white sturgeon have been recaptured. In the Snake River, white sturgeon ranged in total length from 48 cm to 271 cm and averaged 107 cm. In the Salmon River, white sturgeon ranged in total length from 103 cm to 227 cm and averaged 163 cm. Using the Jolly-Seber open population estimator, the abundance of white sturgeon <60 cm, between Lower Granite Dam and the mouth of the Salmon River, was estimated at 2,725 fish, with a 95% confidence interval of 1,668-5,783. A total of 10 white sturgeon were fitted with radio-tags. The movement of these fish ranged from 54.7 km (34 miles) downstream to 78.8 km (49 miles) upstream; however, 43.6 percent of the detected movement was less than 0.8 km (0.5 mile). Both radio-tagged fish and recaptured white sturgeon in Lower Granite Reservoir appear to move more than fish in the free-flowing segment of the Snake River. No seasonal movement pattern was detected, and no movement pattern was detected for different size fish. Differences were detected in the length frequency distributions of

  17. Lake Roosevelt White Sturgeon Recovery Project : Annual Progress Report, January 2003 – March 2004.

    Energy Technology Data Exchange (ETDEWEB)

    Howell, Matthew D.; McLellan, Jason G. [Washington Department of Fish and Wildlife

    2009-07-15

    This report summarizes catch data collected from white sturgeon Acipenser transmontanus in Lake Roosevelt during limited setlining and gill netting activities in the fall of 2003, and documents progress toward development of a U.S. white sturgeon conservation aquaculture program for Lake Roosevelt. From 27-30 October, 42 overnight small mesh gill net sets were made between Marcus and Northport, WA for a total catch of 15 juvenile white sturgeon (275-488 mm FL). All sturgeon captured were of Canadian hatchery origin. These fish had been previously released as sub-yearlings into the Canadian portion (Keenleyside Reach) of the Transboundary Reach of the Columbia River during 2002 and 2003. Most sturgeon (n=14) were caught in the most upstream area sampled (Northport) in low velocity eddy areas. Five fish exhibited pectoral fin deformities (curled or stunted). Growth rates were less than for juvenile sturgeon captured in the Keenleyside Reach but condition factor was similar. Condition factor was also similar to that observed in juvenile sturgeon (ages 1-8) captured in the unimpounded Columbia River below Bonneville Dam between 1987-92. From 10-14 November, 28 overnight setline sets were made in the Roosevelt Reach between the confluence of the Spokane River and Marcus Island for a total catch of 17 white sturgeon (94-213 cm FL). Catch was greatest in the most upstream areas sampled, a distribution similar to that observed during a WDFW setline survey in Lake Roosevelt in 1998. The mean W{sub r} index of 110% for fish captured this year was higher than the mean W{sub r} of 91% for fish captured in 1998. Excellent fish condition hindered surgical examination of gonads as lipid deposits made the ventral body wall very thick and difficult to penetrate with available otoscope specula. Acoustic tags (Vemco model V16 coded pingers, 69 kHz, 48-month life expectancy) were internally applied to 15 fish for subsequent telemetry investigations of seasonal and reproductively

  18. Shifting distributions of adult Atlantic sturgeon amidst post-industrialization and future impacts in the Delaware River: a maximum entropy approach.

    Directory of Open Access Journals (Sweden)

    Matthew W Breece

    Full Text Available Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus experienced severe declines due to habitat destruction and overfishing beginning in the late 19(th century. Subsequent to the boom and bust period of exploitation, there has been minimal fishing pressure and improving habitats. However, lack of recovery led to the 2012 listing of Atlantic sturgeon under the Endangered Species Act. Although habitats may be improving, the availability of high quality spawning habitat, essential for the survival and development of eggs and larvae may still be a limiting factor in the recovery of Atlantic sturgeon. To estimate adult Atlantic sturgeon spatial distributions during riverine occupancy in the Delaware River, we utilized a maximum entropy (MaxEnt approach along with passive biotelemetry during the likely spawning season. We found that substrate composition and distance from the salt front significantly influenced the locations of adult Atlantic sturgeon in the Delaware River. To broaden the scope of this study we projected our model onto four scenarios depicting varying locations of the salt front in the Delaware River: the contemporary location of the salt front during the likely spawning season, the location of the salt front during the historic fishery in the late 19(th century, an estimated shift in the salt front by the year 2100 due to climate change, and an extreme drought scenario, similar to that which occurred in the 1960's. The movement of the salt front upstream as a result of dredging and climate change likely eliminated historic spawning habitats and currently threatens areas where Atlantic sturgeon spawning may be taking place. Identifying where suitable spawning substrate and water chemistry intersect with the likely occurrence of adult Atlantic sturgeon in the Delaware River highlights essential spawning habitats, enhancing recovery prospects for this imperiled species.

  19. Population viability analysis of the Endangered shortnose sturgeon

    Energy Technology Data Exchange (ETDEWEB)

    Jager, Yetta [ORNL; Bevelhimer, Mark S [ORNL; Peterson, Douglas L. [University of Georgia, Athens, GA

    2011-07-01

    This study used population viability analysis (PVA) to partition the influences of potential threats to the endangered shortnose sturgeon (Acipenser brevirostrum). A workshop brought together experts to help identify potential threats including groundwater withdrawal, poor water quality, saltwater intrusion, mercury effects, harvest as by-catch, and sedimentation of spawning habitat. During the course of the project, we eliminated some threats and added new ones. Groundwater withdrawal was dismissed after a study failed to identify connection with groundwater and the majority of pumping is from a confined aquifer. We also eliminated activities on Fort Stewart as influences on spawning habitat because any successful spawning must occur upstream of Fort Stewart. We added climate change to the list of threats based on our assessment of temperature effects and expectations of sea-level rise. Our study highlighted the role of populations in nearby rivers in providing metapopulation support, raising the concern that the population in the Ogeechee River acts as a demographic sink. As part of this study, we carried out a field sampling study to analyze effects of training activities on headwater streams. We developed a new methodology for sampling design as part of this effort and used a mixed-modeling approach to identify relationships between land cover-land use, including those associated with military training activity and water quality. We found that tank training was associated with higher suspended sediment and equipment training was associated with higher organic carbon) and water quality. We detected effects of training on suspended sediment and organic carbon. We also carried out a field sampling effort in the Canoochee and Ogeechee Rivers. In the Ogeechee River, we found that dissolved oxygen in 40% of measurements during summer were below 4 mg L-1. To evaluate mercury as a potential threat, we developed a mercury uptake model and analyzed mercury levels in

  20. Summary of Stock Identification Research on White Sturgeon of the Columbia River, 1985-1991 Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Setter, Ann L.; Brannon, E.L.

    1992-01-01

    White sturgeon (Acipenser transmontanus) are a long-lived, primitive fish species which forage primarily along the river bottom of large river systems in the Pacific Northwest. Historically, as an anadromous species, they could distribute downstream to feed in the rich estuary or marine areas and then migrate back up the river to spawn. With the historic river becoming a series of flooded impoundments, sturgeon were denied open river access, but they appear to have been able to adapt to the altered environment. White sturgeon are found throughout the Columbia River and are thought to be successfully reproducing in some of the impoundments. In those reservoirs where little or no reproduction takes place, enhancement hatcheries may be an option for use in rebuilding isolated populations. However, the degree of stock specificity that exists in the Columbia River was unknown and precluded the use of the more abundant lower river fish as a common egg source to repropagate the upper river unless genetic similarity could be demonstrated among sturgeon throughout the river system. To resolve the issue, research was conducted to determine what level of genetic differentiation exists among sturgeon in the Columbia River system, using starch gel electrophoresis to enable a baseline of population genetic structure data to be assembled. A greater diversity in electrophoretic pattern was observed in the lower portions of the river. The bulk of the qualitative variability we noted was consistent throughout all sections of the river. Some specific quantitative differences were apparent between the areas we examined. Interpretation of the results was complicated by the fact that dam construction would tend to isolate and mix stocks by preventing the migration of fish returning upstream.

  1. Evaluate Potenial Means of Rebuilding Sturgeon Populations in the Snake River between Lower Granite and Hells Canyon Dams, 2002 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Everett, Scott R.; Tuell, Michael A.; Hesse, Jay A. (Nez Perce Tribe, Department of Fisheries Management, Lapwai, ID)

    2004-02-01

    The specific research goal of this project is to identify means to restore and rebuild the Snake River white sturgeon (Acipenser transmontanus) population to support a sustainable annual subsistence harvest equivalent to 5 kg/ha/yr (CBFWA 1997). Based on data collected, a white sturgeon adaptive management plan will be developed. This report presents a summary of results from the 1997-2002 Phase II data collection and represents the end of phase II. From 1997 to 2001 white sturgeon were captured, marked, and population data were collected in the Snake and Salmon. A total of 1,785 white sturgeon were captured and tagged in the Snake River and 77 in the Salmon River. Since 1997, 25.8 percent of the tagged white sturgeon have been recaptured. Relative density of white sturgeon was highest in the free-flowing segment of the Snake River, with reduced densities of fish in Lower Granite Reservoir, and low densities the Salmon River. Differences were detected in the length frequency distributions of white sturgeon in Lower Granite Reservoir, the free-flowing Snake River and the Salmon River (Chi-Square test, P<0.05). The proportion of white sturgeon greater than 92 cm (total length) in the free-flowing Snake River has shown an increase of 30 percent since the 1970's. Using the Jolly-Seber model, the abundance of white sturgeon <60 cm, between Lower Granite Dam and the mouth of the Salmon River, was estimated at 2,483 fish, with a 95% confidence interval of 1,208-7,477. Total annual mortality rate was estimated to be 0.14 (95% confidence interval of 0.12 to 0.17). A total of 35 white sturgeon were fitted with radio-tags during 1999-2002. The movement of these fish ranged from 53 km (33 miles) downstream to 77 km (48 miles) upstream; however, 38.8 percent of the detected movement was less than 0.8 km (0.5 mile). Both radio-tagged fish and recaptured white sturgeon in Lower Granite Reservoir appear to move more than fish in the free-flowing segment of the Snake River. No

  2. Sturgeons are pretty polyploid: hybrid and ploidy diversity in sturgeons

    Czech Academy of Sciences Publication Activity Database

    Symonová, Radka; Flajšhans, Martin; Gela, David; Rodina, Marek; Pelikánová, Šárka; Rábová, Marie; Ráb, Petr

    Lisboa: Centro de Biologia Ambiental da Universidade de Lisboa, 2010. s. 13-13. [The International Meeting on the Genetics of Polyploids. 11.11-12.11.2010, Lisboa] Institutional research plan: CEZ:AV0Z50450515 Keywords : sturgeons * polyploid * diversity Subject RIV: EB - Genetics ; Molecular Biology

  3. Status and Habitat Requirements of the White Sturgeon Populations in the Columbia River Downstream from McNary Dam, 1990-1991 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Nigro, Anthony A. (Oregon Department of Fish and Wildlife, Portland, OR)

    1991-09-01

    We report on our effort from April 1990 to March 1991 to describe the life history and population dynamics of white sturgeon Acipenser transmontanus in.John Day Reservoir. We set 1188 set lines and 26 gill nets. We caught 623 white sturgeon with set lines and 236 with gill nets. Catch per unit effort was much higher in areas near the tailrace than in downstream sites. Our setlines were size selective. We recaptured 3 fish released in John Day Reservoir in 1989 and 28 fish released in 1990. Sport and commercial fishermen recovered 62 tags from fish we tagged in Bonneville, The Dalles and John Day reservoirs, 1987-1990. We observed extensive movements of marked sturgeon within the reservoirs. We completed aging of available samples from all three reservoirs from 1987-1990. We aged fish as old as 46 years. Bone marks were observed on 74 of 78 fish previously injected with oxytetracycline and annulus formation was generally complete after June. We estimated parameters in a length-weight equation. About 1.5% of the female white sturgeon we examined to date had early or late vitellogenic eggs and would be expected to spawn the following year.

  4. Diet and growth of 1+ Siberian sturgeon, Acipenser baerii in alternative pond culture

    Czech Academy of Sciences Publication Activity Database

    Adámek, Z.; Prokeš, Miroslav; Baruš, Vlastimil; Sukop, I.

    2007-01-01

    Roč. 7, č. 2 (2007), s. 153-160. ISSN 1303-2712 R&D Projects: GA MZe QH71305 Institutional research plan: CEZ:AV0Z60930519 Keywords : feeding * weight condition * length-weight relationship * Acipenseridae Subject RIV: GL - Fishing http://www.trjfas.org/pdf/issue_7_2/153_160.pdf

  5. Natural diet and growth of 1+ siberian sturgeon Acipenser baerii in pond culture

    Czech Academy of Sciences Publication Activity Database

    Adámek, Z.; Prokeš, Miroslav; Baruš, Vlastimil; Sukop, I.

    Oostende : European Aquaculture Society, 2001 - (Kjorsvik, E.; Stead, S.). s. 5-6 [ Aquaculture Europe 2001. 04.08.2001-07.08.2001, Trondheim] Institutional research plan: CEZ:MSM 126100002 Subject RIV: GL - Fishing

  6. The Sturgeons: Fragile Species Need Conservation

    OpenAIRE

    Serap Ustaoğlu; İbrahim Okumuş

    2004-01-01

    Sturgeon is among the oldest fishes in the world. They are living in natural waters of Europe, Asia and the Northern America for 200 million years. Once abundant in lakes and rivers throughout the Northern Hemisphere, sturgeon stocks are now highly endangered, mostly due to over-harvesting and severe habitat alterations. Sturgeons are among the most valuable aquatic species in the world. They are prized for their delicate flesh and world famous caviar. They have also interesting evolutionary ...

  7. New disease records for hatchery-reared sturgeon. II. Phaeohyphomycosis due to Veronaea botryosa.

    Science.gov (United States)

    Steckler, Natalie K; Yanong, Roy P E; Pouder, Deborah B; Nyaoke, Akinyi; Sutton, Deanna A; Lindner, Jonathan R; Wickes, Brian L; Frasca, Salvatore; Wolf, Jeffrey C; Waltzek, Thomas B

    2014-10-16

    A series of fungal cases in hatchery-reared juvenile and young adult Siberian sturgeon Acipenser baerii and white sturgeon A. transmontanus occurred at production facilities in Florida and California, USA, respectively. Affected fish exhibited abnormal orientation and/or buoyancy, emaciation, coelomic distension, exophthalmos, cutaneous erythema, and ulcerative skin and eye lesions. Necropsies revealed haemorrhage throughout the coelom, serosanguinous coelomic effusion and organomegaly with nodular or cystic lesions in multiple organs. Fungal hyphae were observed in 27 fish (24 A. baerii and 3 A. transmontanus) via microscopic examination of tissue wet mounts and on slides prepared from colonies grown on culture media. Histopathological examination of these infected tissues revealed extensive infiltration by melanised fungal hyphae that were recovered in culture. Phenotypic characteristics and sequencing of the fungal isolates with the use of the internal transcribed spacer region and 28S rRNA gene confirmed the aetiological agent as Veronaea botryosa. To our knowledge, this is the first documentation of V. botryosa infection in fish, although melanised fungi of the closely related genus Exophiala are well-known pathogens of freshwater and marine fishes. PMID:25320035

  8. UNSTABLE CRITICAL EQUILIBRIUM IN THE DYNAMICS OF COMPLEX STRUCTURED POPULATION OF STURGEON

    Directory of Open Access Journals (Sweden)

    V. A. Dubrovskaya

    2016-01-01

    Full Text Available The article deals with the consequences of the existence of special conditions that determine the character of the rapid further development of population processes. The most probable connection of these critical conditions deals with the optimal number of subpopulation groups. These groups are formed by the evolutionary adaptation of the form of restricted access to resources for reproduction. We have proposed a model of population processes, which formalizes the influence of uneven growth rate for two different groups of reproductive sturgeon mortality of migratory fish in the juvenile period of Acipenser gueldenstaedtii development. In the computing environment was investigated the hybrid system, which simulates the effects of the existence of the situation for the population of migrating juvenile disproportionate reduction in the river. Such cases are established by us according to the rapid decline in sturgeon spawning the Volga. For the exploited population threshold effect after the unstable equilibrium with the minimum necessary for the well being of the form number is the count in the implementation of the «collapse». The phenomenon of collapse detached among all scenarios the most valuable long-term degradation of the reproductive group. In addition to the apparent sharp reduction in reproductive performance, threshold effect is reflected in the adverse events previously dominated evolutionary trend for the survival of certain forms of the life cycle of fish in terms of reorganization of the fishery. Informative, that the Volga stellate sturgeon Acipencer stellatus observed some form of non-linearity in the efficiency of spawning and require a different kind of fish-specific mathematical model.

  9. 中华鲟神经内分泌多肽7B2基因克隆及组织特异性表达%Cloning and expression analysis of a neuroendocrine secretory protein 7B2 in Acipenser sinensis

    Institute of Scientific and Technical Information of China (English)

    刘婷; 章龙珍; 张涛; 庄平; 冯广朋; 赵峰; 马境

    2011-01-01

    In order to clone full-length 7B2 gene and characterize its' tissue expression profile in Chinese sturgeon (Acipenser sinensis ), RT-PCR and rapid-amplification of cDNA ends (RACE) were adopted to obtain full-length cDNA. A full-length 986 bp cDNA sequence in A. sinensis was cloned, including a 711 bp complete ORF encoding a 236 amino acids peptide, a 14 bp long 5′-untranslate region and a 261 bp long 3′-untranslate region. The first 43 of the 236 amino acids were 7B2 signal peptide. The 7B2 in A. sinens showed high similarity to that of Danio rerio. The protein analysis showed that the A. sinensis 7B2 had the closest relationship with the D. redo. Futhermore, 7B2 mRNA tissue expressed profile was assessed by semiquantitative RT-PCR. In the nine examined tissues, the 7B2 gene was expressed strongly in brain, weakly in heart, gonad and pancreas, and little in kidney, bowel and skin, but hardly expressed in gill and liver.%以中华鲟(Acipenser sinensis)脑垂体总RNA为模板,采用RT-PCR和RACE方法,获得中华鲟神经内分泌多肽(782)基因的3个重叠片段,测序后拼接得到986 bp全长基因序列,其中包括5端非翻译区(5'-UTR)14 bp、3'端非翻译区(3'-UTR)261 bp和开放阅读框711 bp.翻译编码236个氨基酸.其中前43个氨基酸为782的信号肽.经BLAST比对发现中华鲟782蛋白的同源性与斑马鱼(Danio rerio)的相似性最高为82%.系统发育分析表明,中华鲟与斑马鱼亲缘关系最近.半定量RT-PCR分析表明:在脑中782 mRNA表达量最高,心脏、性腺、胰等组织中表达次之,肠、肾、皮肤等组织中少量表达,肝和鳃几乎不表达.

  10. Bacterial flora of sturgeon fingerling

    International Nuclear Information System (INIS)

    The study on microbial populations is a suitable tool to understand and apply control methods to improve the sanitary level of production in fish breeding and rearing centers, ensure health of sturgeon fingerlings at the time of their release into the rivers and also in the conversation and restoration of these valuable stocks in the Caspian Sea, Iran. A laboratory research based on Austin methods (Austin, B., Austin, D.A. 1993) was conducted for bacterial study on 3 sturgeon species naming A. persicus, A. stellatus and A. nudiventris during different growth stages. Bacterial flora of Acinetobacter, Moraxella, Aeromonas, Vibrio, Edwardsiella, Staphylococcus, Proteus, Yersinia, Pseudomonas and Plesiomonas were determined. The factors which may induce changes in bacterial populations during different stages of fife are the followings: quality of water in rearing ponds, different conditions for growth stages, suitable time for colonization of bacterial flora in rearing pond, water temperature increase in fingerlings size and feeding condition. (author)

  11. Habitat use of juvenile pallid sturgeon and shovelnose sturgeon with implications for water-level management in a downstream reservoir

    Science.gov (United States)

    Gerrity, P.C.; Guy, C.S.; Gardner, W.M.

    2008-01-01

    Natural recruitment of pallid sturgeon Scaphirhynchus albus has not been observed in the Missouri River above Fort Peck Reservoir, Montana, for at least 20 years. To augment the population, age-1 hatchery-reared juvenile pallid sturgeon were released in 1998. The objective of this study was to evaluate the habitat use of these fish and compare it with that of indigenous shovelnose sturgeon S. platorynchus. Twenty-nine juvenile pallid sturgeon and 21 indigenous shovelnose sturgeon were implanted with radio transmitters in 2003 and 2004. The two species showed no differences in habitat use in terms of mean depth, cross-sectional relative depth, longitudinal relative depth, column velocity, bottom velocity, and channel width. However, there were seasonal differences within both species for cross-sectional relative depth, column velocity, and channel width. Both shovelnose sturgeon and juvenile pallid sturgeon were primarily associated with silt and sand substrate. However, shovelnose sturgeon were associated with gravel and cobble substrate more than juvenile pallid sturgeon. Shovelnose sturgeon and juvenile pallid sturgeon both selected reaches without islands and avoided reaches with islands; the two species also selected main-channel habitat and avoided secondary channels. Mean home range was similar between juvenile pallid sturgeon (15 km; 90% confidence interval, ??5.0 km) and shovelnose sturgeon (16.5 km; ??4.7 km). Spatial distribution differed between the two species, with shovelnose sturgeon using upstream areas more often than juvenile pallid sturgeon. Twenty-eight percent of juvenile pallid sturgeon frequented 60 km of lotie habitat that would be inundated by Fort Peck Reservoir at maximum pool. Stocking juvenile pallid sturgeon can successfully augment the wild pallid sturgeon population in the Missouri River above Fort Peck Reservoir, which is crucial to the long-term recovery of the species. However, water-level management in downstream reservoirs such

  12. Identification of bester hybrid and its parental species (♀ Huso huso Linnaeus, 1758 and ♂ Acipenser ruthenus Linnaeus, 1758 by nuclear markers

    Directory of Open Access Journals (Sweden)

    Andreea Dudu

    2015-05-01

    Full Text Available In Romania, sturgeon farming is gaining advance, different species being raised for commercial purposes and for restocking activities. A correct identification of individuals is imposed since severe ecological damages might occur if non-native species or hybrids are used for restocking. Such identification is required also for commercial reasons, the meat and caviar from different species having different prices. The aim of our study was to analyze two sturgeon species, Huso huso and Acipenser ruthenus and their interspecific hybrid - bester, using nuclear markers, in order to set up a molecular method for their accurate identification. The genetic pattern of the species was inferred from the analysis of nine microsatellite loci (LS19, LS34, LS39, LS54, AoxD234, AnacC11, LS68, Aox45 and Aox27 amplified by multiplex PCR reactions. The genotype data were analyzed with GENETIX v4.05 and STRUCTURE. The FCA analysis grouped the individuals in three distinct clusters corresponding to each of the pure species and to the interspecific hybrids. The admixture analysis performed in STRUCTURE also assigned three groups, confirming the results highlighted by FCA. We can conclude that the selected microsatellite markers allow the unambiguously identification of the bester hybrid and its genitor species from Romanian farms.

  13. Status and habitat requirements of the white sturgeon populations in the Columbia River downstream from McNary Dam. Volume 2: Supplemental papers and data documentation; Final report

    International Nuclear Information System (INIS)

    This is the final report for research on white sturgeon Acipenser transmontanus from 1986--92 and conducted by the National Marine Fisheries Service (NMFS), Oregon Department of Fish and Wildlife (ODFW), US Fish and Wildlife Service (USFWS), and Washington Department of Fisheries (WDF). Findings are presented as a series of papers, each detailing objectives, methods, results, and conclusions for a portion of this research. This volume includes supplemental papers which provide background information needed to support results of the primary investigations addressed in Volume 1. This study addresses measure 903(e)(1) of the Northwest Power Planning Council's 1987 Fish and Wildlife Program that calls for ''research to determine the impact of development and operation of the hydropower system on sturgeon in the Columbia River Basin.'' Study objectives correspond to those of the ''White Sturgeon Research Program Implementation Plan'' developed by BPA and approved by the Northwest Power Planning Council in 1985. Work was conducted on the Columbia River from McNary Dam to the estuary

  14. Middle Mississippi River Sturgeon Preliminary Contaminants Investigation

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — We examined the health of forty-one wild caught sturgeon from a reach of the Mississippi River with an organochlorine consumption advisory and a reference site. The...

  15. Age and Heat Stress as Determinants of Telomere Length in a Long-Lived Fish, the Siberian Sturgeon.

    Science.gov (United States)

    Simide, Rémy; Angelier, Frédéric; Gaillard, Sandrine; Stier, Antoine

    2016-01-01

    Telomeres shorten at each cell division due to the end-replication problem but also in response to oxidative stress. Consequently, telomeres shorten with age in many endotherms, and this shortening is accelerated under stressful environmental conditions. Data in ectotherm vertebrates remain scarce so far, so our goal was to review existing data for fish and to test the influence of age and stress on telomere length in a very long-lived fish, the Siberian sturgeon (Acipenser baerii). Our review of the literature revealed age-related telomere shortening in approximately half of the published studies. In the Siberian sturgeon, we found a significant telomere shortening with age, both at the intraindividual level using red blood cells (-12.5% in 16 mo) and at the interindividual level using cross-sectional samples of fin over an age range of 8 yr. We also found that heat stress (30°C) significantly reduced telomere length by 15.0% after only 1 mo of exposure. Our results highlight that both age and stressful environmental conditions might be important determinants of telomere length in fish. PMID:27617363

  16. 复方中药免疫添加剂对史氏鲟生长性能和抗氧化力的影响%The Effects of Chinese Herbal Compound on Growth and Antioxidant Defenses in Amur Sturgeon as Immune Additive

    Institute of Scientific and Technical Information of China (English)

    刘逊; 王荻; 卢彤岩; 李绍戊; 刘红柏

    2012-01-01

    To use the Chinese herbal medicine feed additive in culture of sturgeon, effects of three kinds of Chinese herbal compound on growth and antioxidant defenses of Amur sturgeon {Acipenserschrenckii Brandt} were studied. The three Chinese herbal compounds (compound A, compound B and compound C) were added into the base material as feed additive, and the one-year-old Acipenserschrenckii Brandt were fed with the experimental feed and the basic feed for 35 days. The growth rate of weight was investigated. The activities of SOD, T-AOC, GSH-PX and the content of MDA were measured by the test. The results showed that the Chinese herbal compound could obviously increase the activities of SOD, T-AOC, GSH-PX. In particular, the SOD activity of the serum of three groups was higher than that in the control group significantly. Moreover, Group A and C both exhibited an apparent increase in the SOD activity of the livers. In comparison with control group, the activities of T-AOC of the serum and livers of three groups remained unchanged remarkably through all the test periods; However, the activities of T-AOC had a little gone up. Only the Group B and C showed obviously increase in the activities of CSH-PX of the serum of Group B and C, nevertheless, the activities of GSH-PX of the livers of three groups had no significant differences. Furthermore, the contents of MDA in serum of Amur sturgeon were lower than that in the control group, and there were significantly different in the serum and livers of three groups. Our results evidently the selected prescriptions have the function to promote antioxidant defenses of Amur sturgeon in the different levels.%探讨了复方中药对史鲟生长及抗氧化能的影响,为中药作为免疫添加剂在鲟鱼养殖生产上的应用提供基础.将3种复方中药(分别为方A、方B、方C)作为饲料添加剂加入基础料饲料中,连续饲喂1+龄史氏鲟(Acipenserschrenckii Brandt)35天,测定增重率并检测血清、肝脏的

  17. Early life history stages of Gulf sturgeon in the Suwannee River, Florida

    Science.gov (United States)

    Sulak, K.J.; Clugston, J.P.

    1998-01-01

    Egg sampling confirmed that Suwannee River Gulf sturgeon Acipenser oxyrinchus desotoi, a subspecies of Atlantic sturgeon A. o. oxyrinchus use the same spawning site at river kilometer (rkm) 215 from the month of the river each year. Forty-nine eggs were recorded in 1995, and 368 were recorded in 1996. Spawning began 4-7 d after the March new moon in both years and lasted 10-11 d; in 1996, a second 10-d spawning round began on the April new moon. Developmental synchrony among eggs recovered suggested several discrete spawning events in both years. Total eggs deposited for three 1996 sampling days was estimated as 405,600-711,000/d, approximating the fecundity range of a large female Gulf sturgeon. Eggs were found only in the southern half of the river, an area with surface currents of 0.5-1.5 m/s and numerous eddies producing reverse bottom currents of 0.1-0.5 m/s. Egg substrate consisted of bedrock limestone thinly overlain by fine sand and densely distributed elliptical gravel 2-10 cm in diameter. Eggs were found predominantly in depths of 2-4 m at water temperatures of 17-21??C, conductivities of 50-100 ??S, and dissolved oxygen levels exceeding 5.0 mg/L. The Cody Scarp, 15 rkm above the spawning ground, may mark the upstream limit of spawning areas in the river. Three 2-4-month-old riverine juveniles (82-115 mm total length, TL) collected are the smallest yet captured from any river. Data for 18 riverine age-0 juveniles (to 350 mm TL) suggest that this stage lasts 6-10 months, terminating with migration of fish to the river mouth in January-February. Less than 2% of 461 juveniles captured at the estuarine river mouth (1990-1993) were under 350 mm TL. Riverine age-0 fish were collected over long shallow stretches (typically <4 m deep) of relatively barren sand (rkm 12-238).

  18. Sediment cores and chemistry for the Kootenai River White Sturgeon Habitat Restoration Project, Boundary County, Idaho

    Science.gov (United States)

    Barton, Gary J.; Weakland, Rhonda J.; Fosness, Ryan L.; Cox, Stephen E.; Williams, Marshall L.

    2012-01-01

    The Kootenai Tribe of Idaho, in cooperation with local, State, Federal, and Canadian agency co-managers and scientists, is assessing the feasibility of a Kootenai River habitat restoration project in Boundary County, Idaho. This project is oriented toward recovery of the endangered Kootenai River white sturgeon (Acipenser transmontanus) population, and simultaneously targets habitat-based recovery of other native river biota. Projects currently (2010) under consideration include modifying the channel and flood plain, installing in-stream structures, and creating wetlands to improve the physical and biological functions of the ecosystem. River restoration is a complex undertaking that requires a thorough understanding of the river. To assist in evaluating the feasibility of this endeavor, the U.S. Geological Survey collected and analyzed the physical and chemical nature of sediment cores collected at 24 locations in the river. Core depths ranged from 4.6 to 15.2 meters; 21 cores reached a depth of 15.2 meters. The sediment was screened for the presence of chemical constituents that could have harmful effects if released during restoration activities. The analysis shows that concentrations of harmful chemical constituents do not exceed guideline limits that were published by the U.S. Army Corps of Engineers in 2006.

  19. Status and Habitat Requirements of the White Sturgeon Populations in the Columbia River Downstream from McNary Dam Volume II; Supplemental Papers and Data Documentation, 1986-1992 Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Beamesderfer, Raymond C.; Nigro, Anthony A. [Oregon Dept. of Fish and Wildlife, Clackamas, OR (US)

    1995-01-01

    This is the final report for research on white sturgeon Acipenser transmontanus from 1986--92 and conducted by the National Marine Fisheries Service (NMFS), Oregon Department of Fish and Wildlife (ODFW), US Fish and Wildlife Service (USFWS), and Washington Department of Fisheries (WDF). Findings are presented as a series of papers, each detailing objectives, methods, results, and conclusions for a portion of this research. This volume includes supplemental papers which provide background information needed to support results of the primary investigations addressed in Volume 1. This study addresses measure 903(e)(1) of the Northwest Power Planning Council's 1987 Fish and Wildlife Program that calls for ''research to determine the impact of development and operation of the hydropower system on sturgeon in the Columbia River Basin.'' Study objectives correspond to those of the ''White Sturgeon Research Program Implementation Plan'' developed by BPA and approved by the Northwest Power Planning Council in 1985. Work was conducted on the Columbia River from McNary Dam to the estuary.

  20. Plasma cholinesterase activity as a biomarker for quantifying exposure of green sturgeon to carbaryl following applications to control burrowing shrimp in Washington State.

    Science.gov (United States)

    Troiano, Alexandra T; Grue, Christian E

    2016-08-01

    Willapa Bay (Washington State, USA) has been 1 of the rare intertidal locations where large-scale pesticide applications occur. Until recently, carbaryl was applied to control burrowing shrimp that decrease commercial oyster productivity. The bay is a critical habitat for green sturgeon (Acipenser medirostris), an anadromous species listed as threatened under the US Endangered Species Act. However, the hazard that carbaryl poses is unknown. Surrogate seawater-acclimated white sturgeon (A. transmontanus) were exposed to 0 μg L(-1) , 30 μg L(-1) , 100 μg L(-1) , 300 μg L(-1) , 1000 μg L(-1) , and 3000 μg L(-1) carbaryl for 6 h, and brain acetylcholinesterase (AChE) and plasma butyrylcholinesterase (BChE) activities were measured. Enzyme recovery was measured in an additional cohort exposed to 1000 μg L(-1) carbaryl for 6 h. Activity of AChE was reduced (p ≤ 0.001) at concentrations ≥ 100 μg L(-1) with recovery in the 1000 μg L(-1) cohort by 72 h. Surprisingly, BChE activity was greater than controls at concentrations ≥ 300 μg L(-1) (p > 0.05), a finding confirmed in additional fish exposed to 3000 μg L(-1) for 6 h (+30%, p recovery by 48 h. Plasma samples were collected from free-living green sturgeon before and 4 d to 5 d after application of carbaryl in Willapa Bay. Activity of BChE after application was reduced 28% (p < 0.001), indicating exposure to the pesticide. However, the lack of congruence between BChE and AChE activity in captive white sturgeon exposed to carbaryl indicates that further studies are needed to better understand the risk carbaryl exposure poses to green sturgeon. Environ Toxicol Chem 2016;35:2003-2015. © 2015 SETAC. PMID:26678014

  1. Growth of sterlet Acipenser ruthenus under experimental and farm conditions of the Czech Republic, with remarks on other sturgeons

    Czech Academy of Sciences Publication Activity Database

    Prokeš, Miroslav; Baruš, Vlastimil; Mareš, J.; Peňáz, Milan; Baránek, V.

    2011-01-01

    Roč. 59, č. 6 (2011), s. 281-290. ISSN 1211-8516 R&D Projects: GA MZe QH71305 Institutional research plan: CEZ:AV0Z60930519 Keywords : sterlet juveniles and adults * specific growth rate * length-weight relationship * condition factor Subject RIV: GL - Fishing http://www.mendelu.cz/dok_server/slozka.pl?id=51329;download=85841

  2. The influence of dietary fatty acid composition on the respiratory and cardiovascular physiology of Adriatic sturgeon (Acipenser naccarii): a review

    DEFF Research Database (Denmark)

    McKenzie, DJ; Piraccini, G; Agnisola, C;

    1999-01-01

    as 15% of dry feed weight), with an elevated content of highly unsaturated fatty acids of the co3 series (¿3 HUFA), had a significantly lower standard metabolic rate (SMR) and routine oxygen consumption (Mo2) than those fed a diet enriched with the same quantity of hydrogenated coconut oil...

  3. Effects of arabinoxylan-oligosaccharides (AXOS) on juvenile Siberian sturgeon (Acipenser baerii) performance, immune responses and gastrointestinal microbial community

    NARCIS (Netherlands)

    Geraylou, Z.; Souffreau, C.; Rurangwa, E.; Hondt, D' S.; Callewaert, L.; Courtin, C.M.; Delcour, J.A.; Buyse, J.; Ollevier, F.

    2012-01-01

    Arabinoxylan-oligosaccharides (AXOS) are a newly discovered class of candidate prebiotics that exert different properties depending on their structure. In this study the effects of two different structures of AXOS, namely AXOS-32-0.30 (average degree of polymerization: 32, average degree of substitu

  4. Defining winter trophic habitat of juvenile Gulf Sturgeon in the Suwannee and Apalachicola rivermouth estuaries, acoustic telemetry investigations

    Science.gov (United States)

    Sulak, K.J.; Randall, M.T.; Edwards, R.E.; Summers, T.M.; Luke, K.E.; Smith, W.T.; Norem, A.D.; Harden, William M.; Lukens, R.H.; Parauka, F.; Bolden, S.; Lehnert, R.

    2009-01-01

    Three automated listening post-telemetry studies were undertaken in the Suwannee and Apalachicola estuaries to gain knowledge of habitats use by juvenile Gulf Sturgeons (Acipenser oxyrinchus desotoi) on winter feeding grounds. A simple and reliable method for external attachment of small acoustic tags to the dorsal fin base was developed using shrink-tubing. Suspending receivers on masts below anchored buoys improved reception and facilitated downloading; a detection range of 500–2500 m was realized. In the Apalachicola estuary, juvenile GS stayed in shallow water (< 2 m) within the estuarine transition zone all winter in the vicinity of the Apalachicola River mouth. Juvenile GS high-use areas did not coincide with high density benthic macrofauna areas from the most recent (1999) benthos survey. In the Suwannee estuary, juveniles ranged widely and individually throughout oligohaline to mesohaline subareas of the estuary, preferentially using mesohaline subareas seaward of Suwannee Reef (52% of acoustic detections). The river mouth subarea was important only in early and late winter, during the times of adult Gulf Sturgeon migrations (41% of detections). Preferred winter feeding subareas coincided spatially with known areas of dense macrofaunal benthos concentrations. Following a dramatic drop in air and water temperatures, juvenile GS left the river mouth and estuary, subsequently being detected 8 km offshore in polyhaline open Gulf of Mexico waters, before returning to the estuary. Cold-event offshore excursions demonstrate that they can tolerate full-salinity polyhaline waters in the open Gulf of Mexico, for at least several days at a time. For juvenile sturgeons, the stress and metabolic cost of enduring high salinity (Jarvis et al., 2001; McKenzie et al., 2001; Singer and Ballantyne, 2002) for short periods in deep offshore waters seems adaptively advantageous relative to the risk of cold-event mortality in shallow inshore waters of lower salinity. Thus

  5. Sensitivity of early life stages of white sturgeon, rainbow trout, and fathead minnow to copper.

    Science.gov (United States)

    Vardy, David W; Oellers, Johanna; Doering, Jon A; Hollert, Henner; Giesy, John P; Hecker, Markus

    2013-01-01

    Populations of white sturgeon (WS; Acipenser transmontanus) are in decline in several parts of the United States and Canada, attributed primarily to poor recruitment caused by degradation of habitats, including pollution with contaminants such as metals. Little is known about sensitivity of WS to contaminants or metals such as copper (Cu). Here, acute (96 h) mortalities of WS early life stages due to exposure to Cu under laboratory conditions are reported. Two standard test species, rainbow trout (Oncorhynchus mykiss) and fathead minnow (Pimephales promelas), were exposed in parallel to determine relative sensitivity among species. Swim-up larvae [15 days post-hatch (dph)] and early juveniles (40-45 dph) of WS were more sensitive to Cu (LC(50) = 10 and 9-17 μg/L, respectively) than were yolksac larvae (8 dph; LC(50) = 22 μg/L) and the later juvenile life stage (100 dph; LC(50) = 54 μg/L). WS were more sensitive to Cu than rainbow trout and fathead minnow at all comparable life stages tested. Yolksac larvae of rainbow trout and fathead minnow were 1.8 and 4.6 times, respectively, more tolerant than WS, while swim-up and juvenile life stages of rainbow trout were between 1.4- and 2.4-times more tolerant than WS. When plotted in a species sensitivity distribution with other fishes, the mean acute toxicity value for early life stage WS was ranked between the 1st and 2nd centile. The WS life stage of greatest Cu sensitivity coincides with the beginning of active feeding and close association with sediment, possibly increasing risk. WS early life stages are sensitive to aqueous copper exposure and site-specific water quality guidelines and criteria should be evaluated closely to ensure adequate protection. PMID:23124699

  6. A health risk evaluation for pallid sturgeon (Scaphirhynchus albus) in the Lower Platte River using shovelnose sturgeon (Scaphirhynchus platorynchus) as a Surrogate

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Most sturgeon species worldwide have been in a steep declince since the 1900s. This research evaluated shovelnose sturgeon health, reproduction, and exposure to...

  7. Application of new phenolic antioxidants for cryopreservation of sturgeon sperm.

    Science.gov (United States)

    Osipova, V P; Berberova, N T; Gazzaeva, R A; Kudryavtsev, K V

    2016-04-01

    Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects of BHT and water-soluble analogue of vitamin E, trolox. The most efficient antioxidant has the effective concentration of 0.1 mM. Novel antioxidant agents as cryomedium supplements not only reduced the level of lipid peroxidation, but also enhanced the translational motility of the sperm of the Russian sturgeon after defrosting. PMID:26929990

  8. Factors Affecting the Reproduction, Recruitment, Habitat, and Population Dynamics of Pallid Sturgeon and Shovelnose Sturgeon in the Missouri River

    Science.gov (United States)

    Korschgen, Carl E., (Edited By)

    2007-01-01

    For more than a hundred years, human activities have modified the natural forces that control the Missouri River and its native fish fauna. While the ecological effects of regulation and channel engineering are understood in general, the current understanding is not sufficient to guide river restoration and management. The U.S. Geological Survey (USGS) is in the third year of a multiagency research effort to determine the ecological requirements for reproduction and survival of the endangered pallid sturgeon (Scaphirhynchus albus) and shovelnose sturgeon (Scaphirhynchus platorhynchus) in the Missouri River. The multidisciplinary research strategy includes components of behavior, physiology, habitat use, habitat availability, and population modeling of all life stages. Shovelnose sturgeon are used to design the strategy because they are closely related to the pallid sturgeon and are often used as a surrogate species to develop new research tools or to examine the effects of management actions or environmental variables on sturgeon biology and habitat use. During fiscal years 2005 and 2006, the U.S. Army Corps of Engineers (USACE) provided funds to USGS for tasks associated with the Comprehensive Sturgeon Research Program (CSRP) and for tasks associated with evaluation of the Sturgeon Response to Flow Modifications (SRFM). Because work activities of CSRP and SRFM are so integrated, we are providing information on activities that have been consolidated at the task level. These task activities represent chapters in this report.

  9. Optimal design of artificial reefs for sturgeon

    Science.gov (United States)

    Yarbrough, Cody; Cotel, Aline; Kleinheksel, Abby

    2015-11-01

    The Detroit River, part of a busy corridor between Lakes Huron and Erie, was extensively modified to create deep shipping channels, resulting in a loss of spawning habitat for lake sturgeon and other native fish (Caswell et al. 2004, Bennion and Manny 2011). Under the U.S.- Canada Great Lakes Water Quality Agreement, there are remediation plans to construct fish spawning reefs to help with historic habitat losses and degraded fish populations, specifically sturgeon. To determine optimal reef design, experimental work has been undertaken. Different sizes and shapes of reefs are tested for a given set of physical conditions, such as flow depth and flow velocity, matching the relevant dimensionless parameters dominating the flow physics. The physical conditions are matched with the natural conditions encountered in the Detroit River. Using Particle Image Velocimetry, Acoustic Doppler Velocimetry and dye studies, flow structures, vorticity and velocity gradients at selected locations have been identified and quantified to allow comparison with field observations and numerical model results. Preliminary results are helping identify the design features to be implemented in the next phase of reef construction. Sponsored by NOAA.

  10. Contaminant evaluation of shovelnose sturgeon from the Atchafalaya River, Louisiana

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Baseline contaminant levels in shovelnose sturgeon (Scaphirynchus platorynchus) collected from the outfall channel of the Old River Control Structure (ORCS),...

  11. Gulf sturgeon Critical Habitat Units 1-7

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data represent the critical habitat for Gulf Sturgeon as designated by Federal Register Vol. 68, No. 53, Wednesday, March 19, 2003, Rules and Regulations.

  12. White Sturgeon Distribution, Pacific Northwest (updated March, 2006)

    Data.gov (United States)

    Pacific States Marine Fisheries Commission — This dataset is a record of fish distribution and activity for WHITE STURGEON contained in the StreamNet database. This feature class was created based on linear...

  13. Gulf sturgeon Critical Habitat Units 8-14

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data represent the critical habitat for Gulf Sturgeon as designated by Federal Register Vol. 68, No. 53, Wednesday, March 19, 2003, Rules and Regulations.

  14. Conservation of caspian sturgeons: some questions and suggestions

    OpenAIRE

    Williot, P.

    1998-01-01

    This paper, focused on sturgeon, is the support of a table given during the first Caspian bio-network workshop organised by the World Bank. The objectives were both preservation and related search of income. Due to the work environment in this region, aquaculture seems to be the only viable way. Following a brief presentation of published sturgeon knowledge in this field, the author describe the different routes for farmed brood stock development and point out some suggestions for biological ...

  15. Management applications of genetic structure of anadromous sturgeon populations in the Lower Danube River (LDR, Romania

    Directory of Open Access Journals (Sweden)

    ONĂRĂ Dalia Florentina

    2013-12-01

    Full Text Available During the last decades, the over-exploitation of sturgeon stocks for caviar production simultaneously with severe habitat deteriorations has led to drastic declines in the natural populations of the Danube River. As a result of (i decrease of sturgeon catches from 37.5 tons in year 2002 to 11.8 tons in year 2005, (ii disrupted age class structure of sturgeon adult cohorts in years 2003 and 2004, and (iii lack or low recruitment in the period 2001 – 2004, in 2005 the Romanian Government started the Supportive Stocking Program of Lower Danube River with hatchery-produced young sturgeons in Romania. Subsequently, in 2006 the commercial sturgeon fishing in Romania was banned for a 10-year period. Genetic investigations were undertaken as an attempt to assess the genetic variability of the sturgeon brood fish, captured from the wild, used in two aquaculture facilities in Romania for obtaining juveniles for supportive stocking of LDR with young sturgeons produced by artificial propagation in year 2007. Our data indicate strong genetic diversity in case of stellate sturgeon and lack of diversity within the batch of beluga sturgeon brood fish captured in 2007, analyzed in the current study. Specific measures that could improve the management plan of sturgeon brood fish in the Romanian part of LDR in the light of recent FAO guidelines regarding the sturgeon hatchery practices and management for release were suggested

  16. Kootenai River White Sturgeon Investigations and Experimental Culture, 1990-1991 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Apperson, Kimberly A.

    1992-07-01

    Setline and angling techniques were used to sample 56 white sturgeon Acioenser transmontanus from the Kootenai River in 1991. Of those sampled, nine were recaptures from previous years of this study. A total of 382 white sturgeon were captured from March 1989 through October 1991. Fork lengths of white sturgeon in the sample ranged from 88-274 cm. Our data indicated there was a complete lack of recruitment of juveniles into the population. The youngest fish sampled was of the 1977 year class. The population was estimated at 880 individuals with a 95% confidence interval of 638 to 1,211. Annual mortality of white sturgeon since 1982 is 3.74%. Approximately 80% of the population was more than 20 years old and was reproductively mature. Surgical examination of 309 white sturgeon since 1989 indicated that approximately 7% of the female white sturgeon and 30% of the male white sturgeon are reproductive each year. The ratio of males to females was estimated at 1:l. White sturgeon sampled and released with and without surgical examination were recaptured at equal rates. An ongoing sonic telemetry study has documented long distance movements by adults. White sturgeon regularly move across the British Columbia - Idaho border. White sturgeon seek out deep holes in the river or migrate to Kootenay Lake during late fall, During spring and early summer of both 1990 and 1991 reproductively mature white sturgeon moved from 15 to 110 km upriver and congregated within 10 km downriver from Bonners Ferry in areas of elevated water velocity. This behavior coincided with increasing discharge and water temperatures. Developing white sturgeon eggs were recovered from the river near Bonners Ferry on July 3, 1991. Contamination of eggs by organochloride compounds were less in recent samples from the Kootenai River than in a single sample collected in 1982. White sturgeon eggs from the Kootenai River fish contained approximately one tenth the organochloride compounds of white sturgeon eggs

  17. High Conservation in Transcriptomic and Proteomic Response of White Sturgeon to Equipotent Concentrations of 2,3,7,8-TCDD, PCB 77, and Benzo[a]pyrene.

    Science.gov (United States)

    Doering, Jon A; Tang, Song; Peng, Hui; Eisner, Bryanna K; Sun, Jianxian; Giesy, John P; Wiseman, Steve; Hecker, Markus

    2016-05-01

    Adverse effects associated with exposure to dioxin-like compounds (DLCs) are mediated primarily through activation of the aryl hydrocarbon receptor (AHR). However, little is known about the cascades of events that link activation of the AHR to apical adverse effects. Therefore, this study used high-throughput, next-generation molecular tools to investigate similarities and differences in whole transcriptome and whole proteome responses to equipotent concentrations of three agonists of the AHR, 2,3,7,8-TCDD, PCB 77, and benzo[a]pyrene, in livers of a nonmodel fish, the white sturgeon (Acipenser transmontanus). A total of 926 and 658 unique transcripts were up- and down-regulated, respectively, by one or more of the three chemicals. Of the transcripts shared by responses to all three chemicals, 85% of up-regulated transcripts and 75% of down-regulated transcripts had the same magnitude of response. A total of 290 and 110 unique proteins were up- and down-regulated, respectively, by one or more of the three chemicals. Of the proteins shared by responses to all three chemicals, 70% of up-regulated proteins and 48% of down-regulated proteins had the same magnitude of response. Among treatments there was 68% similarity between the global transcriptome and global proteome. Pathway analysis revealed that perturbed physiological processes were indistinguishable between equipotent concentrations of the three chemicals. The results of this study contribute toward more completely describing adverse outcome pathways associated with activation of the AHR. PMID:27070345

  18. Fine structure and morphology of sterlet (Acipenser ruthenus L. 1758) spermatozoa and acrosin localization

    Czech Academy of Sciences Publication Activity Database

    Pšenička, M.; Vančová, M.; Koubek, Pavek; Těšitel, J.; Linhart, O.

    2009-01-01

    Roč. 111, č. 1 (2009), s. 3-16. ISSN 0378-4320 R&D Projects: GA ČR(CZ) GA524/06/0817 Institutional research plan: CEZ:AV0Z50520701 Keywords : Acipenser ruthenus * Acrosome * Acrosin * Morphometric analysis * SEM * TEM Subject RIV: EG - Zoology Impact factor: 1.563, year: 2009

  19. Effects of commercial harvest on shovelnose sturgeon populations in the Upper Mississippi River

    Science.gov (United States)

    Koch, Jeff D.; Quist, Michael C.; Pierce, Clay L.; Hansen, Kirk A.; Steuck, Michael J.

    2009-01-01

    Shovelnose sturgeon Scaphirhynchus platorynchus have become an increasingly important commercial species in the upper Mississippi River (UMR) because of the collapse of foreign sturgeon (family Acipenseridae) populations and bans on imported caviar. In response to concerns about the sustainability of the commercial shovelnose sturgeon fishery in the UMR, we undertook this study to describe the demographics of the shovelnose sturgeon population and evaluate the influence of commercial harvest on shovelnose sturgeon populations in the UMR. A total of 1,682 shovelnose sturgeon were collected from eight study pools in 2006 and 2007 (Pools 4, 7, 9, 11, 13, 14, 16, and 18). Shovelnose sturgeon from upstream pools generally had greater lengths, weights, and ages than those from downstream pools. Additionally, mortality estimates were lower in upstream pools (Pools 4, 7, 9, and 11) than in downstream pools (Pools 13, 14, 16, and 18). Linear regression suggested that the slower growth of shovelnose sturgeon is a consequence of commercial harvest in the UMR. Modeling of potential management scenarios suggested that a 685-mm minimum length limit is necessary to prevent growth and recruitment overfishing of shovelnose sturgeon in the UMR.

  20. Laboratory evaluation of effects of early life-stage exposure to selenium on pallid and shovelnose sturgeon

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Endangered pallid sturgeon and congeneric shovelnose sturgeon found in the lower Platte River, NE and elsewhere in the Missouri River basin have elevated tissue...

  1. Comparative analysis of morphometric characters of juvenile sterlet Acipenser ruthenus L. from natural population and aquaculture

    Czech Academy of Sciences Publication Activity Database

    Lenhardt, M.; Prokeš, Miroslav; Jaric, I. Z.; Baruš, Vlastimil; Kolarevic, J.; Krupka, I.; Cvijanovic, G.; Cakic, Z.; Gacic, Z.

    2004-01-01

    Roč. 65, Suppl.A (2004), s. 320. ISSN 0022-1112. [Nature and culture: comparative biology and interactions of wild and farmed fish. London, 19.07.2004-23.07.2004] R&D Projects: GA MZe(CZ) QF3028 Institutional research plan: CEZ:AV0Z6093917 Keywords : Acipenser ruthenus * morphometric characters Subject RIV: GL - Fishing Impact factor: 1.198, year: 2004

  2. Programa de recuperación para Acipenser sturio L., 1758: la propuesta alemana

    OpenAIRE

    Kirschbaum, F.; Gessner, J.

    2001-01-01

    Acipenser sturio L., 1758 was a prevalent species of the fish communities in all major German rivers' waters until the end of the 19th century. Since then, the population sizes decreased rapidly due to overfishing, pollution, and hydro-construction, and the last remaining population was observed in the Eider River until the 1960s. Recently, specimens have been observed in German waters only rarely. Until the end of the 1980s, neither politicians nor ecologists considered reestablishment measu...

  3. Novel single-nucleotide polymorphism markers confirm successful spawning of endangered pallid sturgeon in the upper Missouri River Basin

    Science.gov (United States)

    Eichelberger, Jennifer S.; Braaten, P. J.; Fuller, D. B.; Krampe, Matthew S.; Heist, Edward J.

    2014-01-01

    Spawning of the federally endangered Pallid Sturgeon Scaphirhynchus albus is known to occur in the upper Missouri River basin, but progeny from natural reproductive events have not been observed and recruitment to juvenile or adult life stages has not been documented in recent decades. Identification of Pallid Sturgeon progeny is confounded by the fact that Shovelnose Sturgeon S. platorynchus occurs throughout the entire range of Pallid Sturgeon and the two species are essentially indistinguishable (morphometrically and meristically) during early life stages. Moreover, free embryos of sympatric Paddlefish Polyodon spathula are very similar to the two sturgeon species. In this study, three single-nucleotide polymorphism (SNP) assays were employed to screen acipenseriform free embryos and larvae collected from the upper Missouri River basin in 2011, 2012, and 2013. A mitochondrial DNA SNP discriminates Paddlefish from sturgeon, and specific multilocus genotypes at two nuclear DNA SNPs occurred in 98.9% of wild adult Pallid Sturgeon but only in 3% of Shovelnose Sturgeon sampled in the upper Missouri River. Individuals identified as potential Pallid Sturgeon based on SNP genotypes were further analyzed at 19 microsatellite loci for species discrimination. Out of 1,423 free embryos collected over 3 years of sampling, 971 Paddlefish, 446 Shovelnose Sturgeon, and 6 Pallid Sturgeon were identified. Additionally, 249 Scaphirhynchus spp. benthic larvae were screened, but no Pallid Sturgeon were detected. These SNP markers provide an efficient method of screening acipenseriform early life stages for the presence of Pallid Sturgeon in the Missouri River basin. Detection of wild Pallid Sturgeon free embryos in the upper Missouri and Yellowstone rivers supports the hypothesis that the failure of wild Pallid Sturgeon to recruit to the juvenile life stage in the upper Missouri River basin is caused by early life stage mortality rather than by lack of successful spawning.

  4. Ecological requirements for pallid sturgeon reproduction and recruitment in the Lower Missouri River: Annual report 2009

    Science.gov (United States)

    DeLonay, Aaron J.; Jacobson, Robert B.; Papoulias, Diana M.; Wildhaber, Mark L.; Chojnacki, Kimberly A.; Pherigo, Emily K.; Bergthold, Casey L.; Mestl, Gerald E.

    2010-01-01

    The Comprehensive Sturgeon Research Project is a multiyear, multiagency collaborative research framework developed to provide information to support pallid sturgeon recovery and Missouri River management decisions. The general Comprehensive Sturgeon Research Project strategy is to integrate field and laboratory studies of sturgeon reproductive ecology, habitat requirements, and physiology to produce a predictive understanding of sturgeon population dynamics. The project scope of work is developed annually with cooperating research partners and in collaboration with the U.S. Army Corps of Engineers, Missouri River Recovery-Integrated Science Program. The research consists of several interdependent and complementary research tasks engaging multiple disciplines that primarily address spawning as a probable limiting factor in reproduction and survival of the pallid sturgeon. The research is multifaceted and is designed to provide information needed for management decisions impacting habitat restoration, flow modification, and pallid sturgeon population augmentation on the Missouri River, and throughout the range of the species. Research activities and progress towards understanding of the species are reported to the U.S. Army Corps of Engineers annually. This annual report details the research effort and progress made by Comprehensive Sturgeon Research Project during 2009.

  5. A Conceptual Life-History Model for Pallid and Shovelnose Sturgeon

    Science.gov (United States)

    Wildhaber, Mark L.; DeLonay, Aaron J.; Papoulias, Diana M.; Galat, David L.; Jacobson, Robert B.; Simpkins, Darin G.; Braaten, P. J.; Korschgen, Carl E.; Mac, Michael J.

    2007-01-01

    Intensive management of the Missouri and Mississippi Rivers has resulted in dramatic physical changes to these rivers. These changes have been implicated as causative agents in the decline of pallid sturgeon. The pallid sturgeon, federally listed as endangered, is endemic to the turbid waters of the Missouri River and the Lower Mississippi River. The sympatric shovelnose sturgeon historically was more common and widespread than the pallid sturgeon. Habitat alteration, river regulation, pollution, and over-harvest have resulted in the now predictable patterns of decline and localized extirpation of sturgeon across species and geographic areas. Symptomatic of this generalized pattern of decline is poor reproductive success, and low or no recruitment of wild juveniles to the adult population. The purpose of this report is to introduce a conceptual life-history model of the factors that affect reproduction, growth, and survival of shovelnose and pallid sturgeons. The conceptual model provided here was developed to organize the understanding about the complex life history of Scaphirhynchus sturgeons. It was designed to be used for communication, planning, and to provide the structure for a population-forecasting model. These models are intended to be dynamic and responsive to new information and changes in river management, thereby providing scientists, stakeholders, and managers with ways to improve understanding of the effects of management actions on the ecological requirements of Scaphirhynchus sturgeons. As new scientific knowledge becomes available, it could be included in the model in many ways at various integration levels.

  6. Effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, Acipenser ruthenus

    DEFF Research Database (Denmark)

    Siddique, Mohammad Abdul Momin; Butts, Ian; Psěnička, Martin;

    2015-01-01

    than the 430:1 ratio. Generally, for 430:1 ratio, the fertilization rate is lower than in control. Transmission electron microscopy showed that pre-incubation of eggs in water for <10. min does not trigger a cortical reaction or the formation of a perivitelline space. Results suggest that with a low...

  7. Atlantic Sturgeon Spatial and Temporal Distribution in Minas Passage, Nova Scotia, Canada, a Region of Future Tidal Energy Extraction

    Science.gov (United States)

    Stokesbury, Michael J. W.; Logan-Chesney, Laura M.; McLean, Montana F.; Buhariwalla, Colin F.; Redden, Anna M.; Beardsall, Jeffrey W.; Broome, Jeremy E.; Dadswell, Michael J.

    2016-01-01

    In the Bay of Fundy, Atlantic sturgeon from endangered and threatened populations in the USA and Canada migrate through Minas Passage to enter and leave Minas Basin. A total of 132 sub-adult and adult Atlantic sturgeon were tagged in Minas Basin during the summers of 2010–2014 using pressure measuring, uniquely coded, acoustic transmitters with a four or eight year life span. The aim of this study was to examine spatial and seasonal distribution of sturgeon in Minas Passage during 2010–2014 and test the hypothesis that, when present, Atlantic sturgeon were evenly distributed from north to south across Minas Passage. This information is important as tidal energy extraction using in-stream, hydrokinetic turbines is planned for only the northern portion of Minas Passage. Electronic tracking data from a total of 740 sturgeon days over four years demonstrated that Atlantic sturgeon used the southern portion of Minas Passage significantly more than the northern portion. Sturgeon moved through Minas Passage at depths mostly between 15 and 45 m (n = 10,116; mean = 31.47 m; SD = 14.88). Sturgeon mean swimming depth was not significantly related to bottom depth and in deeper regions they swam pelagically. Sturgeon predominately migrated inward through Minas Passage during spring, and outward during late summer-autumn. Sturgeon were not observed in Minas Passage during winter 2012–2013 when monitoring receivers were present. This information will enable the estimation of encounters of Atlantic sturgeon with in-stream hydrokinetic turbines. PMID:27383274

  8. Ecological requirements for pallid sturgeon reproduction and recruitment in the Missouri River—Annual report 2013

    Science.gov (United States)

    Delonay, Aaron J.; Jacobson, Robert B.; Chojnacki, Kimberly A.; Braaten, Patrick J.; Buhl, Kevin J.; Eder, Brandon L; Elliott, Caroline M.; Erwin, Susannah O.; Fuller, David B.; Haddix, Tyler M.; Ladd, Hallie L.A.; Mestl, Gerald E.; Papoulias, Diana M.; Rhoten, Jason C.; Wesolek, Christopher J.; Wildhaber, Mark L.

    2016-01-01

    The Comprehensive Sturgeon Research Project is a multiyear, multiagency collaborative research framework developed to provide information to support pallid sturgeon recovery and Missouri River management decisions. The project strategy integrates field and laboratory studies of pallid sturgeon reproductive ecology, early life history, habitat requirements, and physiology. The project scope of work is developed annually with collaborating research partners and in cooperation with the U.S. Army Corps of Engineers, Missouri River Recovery—Integrated Science Program. The research consists of several interdependent and complementary tasks that engage multiple disciplines.

  9. A model of sturgeon distribution under a dam of a hydro-electric power plant

    International Nuclear Information System (INIS)

    This article is dedicated to the question of passage of sturgeon through a dam of a hydro-electric power plant (HPP) during spawning migrations. A model of distribution of water velocity in the tail water of HPP was constructed. On the basis of this model a model of sturgeon distribution under a dam of HPP was created. The latter model was analyzed numerically in conformity with the largest HPP on the Volga river-Volgograd's HPP (Russia). The results of analysis allowed estimation of sturgeon passage from the tail water to the head race. Measures for increase of effectiveness of this process were elaborated

  10. Population viability analysis and potential of its application to Danube sturgeons

    Directory of Open Access Journals (Sweden)

    Jarić I.

    2009-01-01

    Full Text Available Sturgeon species in the Danube River basin have experienced severe decline. Besides overexploitation, habitat loss, and pollution, they are further endangered by lack of efficient policy and management, as well as by serious lack of knowledge about their life history. Although population viability analysis (PVA could represent an extremely valuable tool to cope with these problems, it has not so far been applied to Danube populations. This paper represents an assess­ment of different PVA methods and models developed for sturgeon species. It analyzes their results, main advantages, drawbacks, and problems, and discusses the possibility of applying PVA to sturgeon populations in the Danube River basin.

  11. Effects of Seismic Air Guns on Pallid Sturgeon and Paddlefish.

    Science.gov (United States)

    Popper, Arthur N; Carlson, Thomas J; Gross, Jackson A; Hawkins, Anthony D; Zeddies, David; Powell, Lynwood; Young, John

    2016-01-01

    Pallid sturgeon and paddlefish were placed at different distances from a seismic air gun array to determine the potential effects on mortality and nonauditory body tissues from the sound from a single shot. Fish were held 7 days postexposure and then necropsied. No fish died immediately after sound exposure or over the postexposure period. Statistical analysis of injuries showed no differences between the experimental and control animals in either type or severity of injuries. There was also no difference in injuries between fish exposed closest to the source compared with those exposed furthest from the source. PMID:26611044

  12. Review of BPA funded sturgeon, resident fish and wildlife projects

    International Nuclear Information System (INIS)

    The Bonneville Power Administration (BPA) held a public meeting on November 19--21, 1991, for the purpose of review, coordination, and consultation of the BPA-funded projects for sturgeon, resident fish, and wildlife in the Columbia River Basin (Basin). The comments received after the meeting were favorable and the participants agreed that the meeting was stimulating and productive. The information exchanged should lead to better coordination with other projects throughout the Basin. This document list the projects by title, the project leaders and BPA's project officers, and an abstract of each leader's presentation

  13. Management and recovery options for Ural river beluga sturgeon.

    Science.gov (United States)

    Doukakis, Phaedra; Babcock, Elizabeth A; Pikitch, Ellen K; Sharov, Alexei R; Baimukhanov, Mirgaly; Erbulekov, Sagiden; Bokova, Yelena; Nimatov, Akhat

    2010-06-01

    Management of declining fisheries of anadromous species sometimes relies heavily on supplementation of populations with captive breeding, despite evidence that captive breeding can have negative consequences and may not address the root cause of decline. The beluga sturgeon (Huso huso), a species threatened by the market for black caviar and reductions in habitat quality, is managed through harvest control and hatchery supplementation, with an emphasis on the latter. We used yield per recruit and elasticity analyses to evaluate the population status and current levels of fishing and to identify the life-history stages that are the best targets for conservation of beluga of the Ural River. Harvest rates in recent years were four to five times higher than rates that would sustain population abundance. Sustainable rates of fishing mortality are similar to those for other long-lived marine species such as sharks and mammals. Yield per recruit, which is maximized if fish are first harvested at age 31 years, would be greatly enhanced by raising minimum size limits or reducing illegal take of subadults. Improving the survival of subadult and adult females would increase population productivity by 10 times that achieved by improving fecundity and survival from egg to age 1 year (i.e., hatchery supplementation). These results suggest that reducing mortality of subadults and adult wild fish is a more effective conservation strategy than hatchery supplementation. Because genetics is not factored into hatchery management practices, supplementation may even reduce the viability of the beluga sturgeon. PMID:20184647

  14. Sedimentology and sequence stratigraphy of the Sturgeon Lake field, Alberta

    Energy Technology Data Exchange (ETDEWEB)

    Mederos, S.M. [Maraven, S.A., Caracas (Venezuela); Moslow, T.F. [Univ. of Alberta, Edmonton (Canada)

    1996-08-01

    This study examines the sedimentology, sequence stratigraphy and reservoir characterization of the Lower Triassic Montney Formation in the Sturgeon Lake field located in west-central Alberta. The Montney Formation is grouped into two facies associations. Facies Association 1 is a siliciclastic upward-coarsening sequence deposited by storm, current and wave processes and is interpreted as a low energy progradational lower shoreface. Facies Association 2 is a carbonate shallowing upward sequence deposited in a wave dominated progradational shoreface. The contact between Facies Association 1 and 2 is marked by a major change in lithology and is erosive. Palynological analyses reveal two missing palynologic subzones between Facies Association 1 and Facies Association 2 suggesting a period of erosion and/or nondeposition. The boundary between the two facies association is defined as a sequence boundary which stratigraphically divides the Montney Formation into two sequences in the study area. The Lower Montney sequence is composed of eight retrogradational, aggradational and progradational parasequences and represent the Transgressive and the High-stand System Tract. The Upper Montney sequence is composed only of one parasequence and represents the Transgressive System Tract. The Sturgeon Lake Field has two types of reservoir with respect to lithology, porosity, permeability and geometry. The best reservoir facies is a brachiopod wackestone-packstone with permeabilities up to 8 Darcys. Siliciclastic reservoirs consist of very fine grained sandstones with permeabilities of 132 md when fractured.

  15. Documentation of a Gulf sturgeon spawning site on the Yellow River, Alabama, USA

    Science.gov (United States)

    Kreiser, Brian R.; Berg, J.; Randall, M.; Parauka, F.; Floyd, S.; Young, B.; Sulak, Kenneth J.

    2008-01-01

    The Gulf Sturgeon Recovery Plan (USFWS, GSMFC and NMFS 1995) stressed the need to provide maximum protection to Gulf sturgeon spawning habitat. The approach employed by various Gulf sturgeon researchers, including ourselves, to document spawning has been to identify potential spawning habitat on the basis of physical characteristics and/or tracking data, collect eggs, and then raise the eggs in the laboratory until the point where the larval fish can be identified (e.g., Marchant and Shutters 1996, Sulak and Clugston 1998, 1999). However, collecting eggs in any appreciable number is usually difficult, and these eggs may not always be viable upon return to the laboratory. Molecular methods provide an alternative means of identifying the species represented by an egg. Notable examples related to sturgeon conservation include cases where molecular markers were used to verify the sources of commercially available caviar (DeSalle and Birstein 1996, Birstein et al. 1999).

  16. Organochlorine chemical hazards for sturgeon larvae in the Middle Mississippi River National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — We conducted an ecological risk assessment to characterize organochlorine chemical hazards for sturgeon larvae in the Middle Mississippi River National Wildlife...

  17. First record of a Polypodium sp. parasitizing eggs of shovelnose sturgeon from the Wabash River, Indiana.

    Science.gov (United States)

    Sepúlveda, Maria S; Stefanavage, Tom; Goforth, Reuben

    2010-03-01

    This article reports the presence of an endocellular parasite, Polypodium sp., in the eggs of shovelnose sturgeon Scaphirhynchus platorynchus from the Wabash River, Indiana. The parasite was detected in 18% (2/11) of adult female sturgeon necropsied in April 2008. This constitutes the first record of this parasite in this host and in Indiana. The implications for the quality of the caviar remain unknown at this time. PMID:20575363

  18. Movements of White Sturgeon in Lake Roosevelt : Final Report 1988-1991.

    Energy Technology Data Exchange (ETDEWEB)

    Brannon, E.L.; Setter, Ann L.

    1992-06-01

    Historically, white sturgeon moved throughout the Columbia River system, ranging freely from the estuary to the headwaters, with the possible exception of limited passage at Cascades, Celilo and Kettle Falls during spring floods. Construction of Rock Island Dam in 1933, followed by Bonneville in 1938 and Grand Coulee in 1941, completely disrupted sturgeon migratory opportunity, and with the 17 successive Columbia and Snake river dams constructed over the next 32 years an entirely different river system was created for the species. Sturgeon caught between dams were essentially isolated populations with severely limited reproduction potential. Some reservoirs ran from dam to dam with no river habitat remaining, while other reaches had various lengths of free running river, but drastically reduced from historical situations. However, if reservoirs provide habitat for sturgeon use, and therefore compensate to some degree for river loss, the major limiting factors associated with population viability may be reduced spawning success, either from lack of suitable area or poor incubation environments. The most upstream impoundment of the Columbia River in the United States is Lake Roosevelt, behind Grand Coulee Dam. If sturgeon don`t use Lake Roosevelt the capacity of the system to sustain a large sturgeon population would be understandably limited, and much reduced from the pre-dam era. In general this study found that sturgeon spawner aggregations from early spring to mid- summer depend most heavily on the timing of increasing water temperature. In the spring the water temperatures seem to stimulate the fish to start feeding and leave deep pools. The summer provides access to broader and shallower areas for food. The study on sturgeon movement was an attempt to define habitat use in such a reservoir/river system.

  19. Ecological requirements for pallid sturgeon reproduction and recruitment in the Missouri River—Annual report 2014

    Science.gov (United States)

    Delonay, Aaron J.; Chojnacki, Kimberly A.; Jacobson, Robert B.; Braaten, Patrick J.; Buhl, Kevin J.; Elliott, Caroline M.; Erwin, Susannah O.; Faulkner, Jacob D.A.; Candrl, James S.; Fuller, David B.; Backes, Kenneth M.; Haddix, Tyler M.; Rugg, Matthew L.; Wesolek, Christopher J.; Eder, Brandon L.; Mestl, Gerald E.

    2016-01-01

    The Comprehensive Sturgeon Research Project is a multiyear, multiagency collaborative research framework developed to provide information to support pallid sturgeon recovery and Missouri River management decisions. The project strategy integrates field and laboratory studies of sturgeon reproductive ecology, early life history, habitat requirements, and physiology. The project scope of work is developed annually with collaborating research partners and in cooperation with the U.S. Army Corps of Engineers, Missouri River Recovery Program–Integrated Science Program. The project research consists of several interdependent and complementary tasks that involve multiple disciplines.The project research tasks in the 2014 scope of work emphasized understanding of reproductive migrations and spawning of adult pallid sturgeon and hatch and drift of larvae. These tasks were addressed in three hydrologically and geomorphologically distinct parts of the Missouri River Basin: the Lower Missouri River downstream from Gavins Point Dam, the Upper Missouri River downstream from Fort Peck Dam and downstream reaches of the Milk River, and the Lower Yellowstone River. The project research is designed to inform management decisions related to channel re-engineering, flow modification, and pallid sturgeon population augmentation on the Missouri River and throughout the range of the species. Research and progress made through this project are reported to the U.S. Army Corps of Engineers annually. This annual report details the research effort and progress made by the Comprehensive Sturgeon Research Project during 2014.

  20. Ecological requirements for pallid sturgeon reproduction and recruitment in the Lower Missouri River: Annual report 2010

    Science.gov (United States)

    DeLonay, Aaron J.; Jacobson, Robert B.; Papoulias, Diana M.; Wildhaber, Mark L.; Chojnacki, Kimberly A.; Pherigo, Emily K.; Haas, Justin D.; Mestl, Gerald E.

    2012-01-01

    The Comprehensive Sturgeon Research Project is a multiyear, multiagency collaborative research framework developed to provide information to support pallid sturgeon recovery and Missouri River management decisions. The project strategy integrates field and laboratory studies of sturgeon reproductive ecology, early life history, habitat requirements, and physiology. The project scope of work is developed annually with cooperating research partners and in collaboration with the U.S. Army Corps of Engineers, Missouri River Recovery—Integrated Science Program. The research consists of several interdependent and complementary tasks that engage multiple disciplines. The research tasks in the 2010 scope of work primarily address spawning as a probable factor limiting pallid sturgeon survival and recovery, although limited pilot studies also have been initiated to examine the requirements of early life stages. The research is designed to inform management decisions affecting channel re-engineering, flow modification, and pallid sturgeon population augmentation on the Missouri River, and throughout the range of the species. Research and progress made through this project are reported to the U.S. Army Corps of Engineers annually. This annual report details the research effort and progress made by the Comprehensive Sturgeon Research Project during 2010.

  1. Ecological requirements for pallid sturgeon reproduction and recruitment in the Missouri River: annual report 2011

    Science.gov (United States)

    DeLonay, Aaron J.; Jacobson, Robert B.; Chojnacki, Kimberly A.; Annis, Mandy L.; Braaten, P. J.; Elliott, Caroline M.; Fuller, D. B.; Haas, Justin D.; Haddix, Tyler M.; Ladd, Hallie L.A.; McElroy, Brandon J.; Mestl, Gerald E.; Papoulias, Diana M.; Rhoten, Jason C.; Wildhaber, Mark L.

    2014-01-01

    The Comprehensive Sturgeon Research Project is a multiyear, multiagency collaborative research framework developed to provide information to support pallid sturgeon recovery and Missouri River management decisions. The project strategy integrates field and laboratory studies of sturgeon reproductive ecology, early life history, habitat requirements, and physiology. The project scope of work is developed annually with cooperating research partners and in collaboration with the U.S. Army Corps of Engineers, Missouri River Recovery—Integrated Science Program. The research consists of several interdependent and complementary tasks that engage multiple disciplines. The research tasks in the 2011 scope of work emphasized understanding of reproductive migrations and spawning of adult sturgeon, and hatch and drift of larvae. These tasks were addressed in three hydrologically and geomorphologically distinct parts of the Missouri River Basin: the Lower Missouri River downstream from Gavins Point Dam, the Upper Missouri River downstream from Fort Peck Dam and including downstream reaches of the Milk River, and the Lower Yellowstone River. The research is designed to inform management decisions related to channel re-engineering, flow modification, and pallid sturgeon population augmentation on the Missouri River, and throughout the range of the species. Research and progress made through this project are reported to the U.S. Army Corps of Engineers annually. This annual report details the research effort and progress made by the Comprehensive Sturgeon Research Project during 2011.

  2. Kootenai River White Sturgeon Investigations and Experimental Culture, 1989-1990 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Apperson, Kimberly A.; Anders, Paul J.

    1991-10-01

    Setline and angling techniques were used to sample 332 sturgeon from the river between Kootenai Falls and Kootenay Lake during 1989 and 1990. Sturgeon were found in Montana within 4 km of Kootenai Falls and downstream from Bonners Ferry, Idaho to Kootenay Lake, British Columbia. Our data indicate there is a complete lack of recruitment of juveniles into the population. The youngest fish sampled was of the 1977 year class, and the population is estimated at 880 individuals with 95% confidence intervals of 638 to 1,211. Culture of one pair of sturgeon in 1990 was of limited success. Less than 5% of eggs hatched with 50% initial mortality of fry. The contribution of contaminants found in eggs (aluminum, copper, zinc, lead, and organochlorides) toward this poor survival is unknown. Handling problems with the eggs at the time of spawning complicated our results. An ongoing sonic telemetry study has revealed definite long distance movements. Sturgeon regularly move across the British Columbia-Idaho border and seek out deep holes or migrate to Kootenay Lake during late fall. Seasonal differences in use of depth and velocity parameters were found between sexes and among seasons. No relationships were found between sturgeon movement and month, water temperature, flow, and flow change. However, multiple regression analysis indicated that up to 30% of the variance in individual sturgeon movement was explained by the combination of the four variables.

  3. La restauration de l'esturgeon européen (Acipenser sturio) et les extractions de granulats dans la Gironde

    OpenAIRE

    Lepage, M.; Rochard, E.; CASTELNAUD G.

    1998-01-01

    The Gironde estuary in the south-west of France is the last estuary where all diadromous fish from western europe still migrate. In addition, the Gironde-Garonne-Dordogne basin is the last know system where the reproduction of the european sturgeon take place. The european sturgeon is an endangered species which is listed under the Threatened species Red Book of France and other international conventions for the conservation of the nature. It is also fully protected in France since 1982 by a ...

  4. White Sturgeon Mitigation and Restoration in the Columbia and Snake Rivers Upstream from Bonneville Dam; 2001-2002 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ward, David L.; Kern, J. Chris; Hughes, Michele L.

    2003-12-01

    We report on our progress from April 2001 through March 2002 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam.

  5. White Sturgeon Mitigation and Restoration in the Columbia and Snake Rivers Upstream from Bonneville Dam; 2002-2003 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ward, David L.; Kern, J. Chris; Hughes, Michele L. (Oregon Department of Fish and Wildlife)

    2004-02-01

    We report on our progress from April 2002 through March 2003 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam.

  6. Effects of feed restriction on the upper temperature tolerance and heat shock response in juvenile green and white sturgeon.

    Science.gov (United States)

    Lee, Seunghyung; Hung, Silas S O; Fangue, Nann A; Haller, Liran; Verhille, Christine E; Zhao, Juan; Todgham, Anne E

    2016-08-01

    The objective of the current study was to investigate the effects of feed restriction on whole-organism upper thermal tolerance and the heat shock response of green and white sturgeon to determine how changes in food amount might influence physiological performance of each species when faced with temperature stress. Two parallel feed restriction trials were carried out for juvenile green (202g; 222-day post hatch: dph) and white sturgeon (205g; 197-dph) to manipulate nutritional status at 12.5%, 25%, 50%, or 100% of optimum feeding rate (100% OFR were 1.6% and 1.8% body weight/day, respectively) for four weeks. Following the trials, the critical thermal maximum (CTMax, 0.3°C/min) of sturgeon (N=12/treatment/species) was assessed as an indicator of whole-organism upper thermal tolerance. To assess temperature sensitivity, sturgeon (N=9/treatment/species) were acutely transferred to two temperature treatments (28°C and 18°C as a handling control) for 2h followed by 2h of recovery at 18°C before being sacrificed, and gill, brain, and mucus sampled for measurements of 70-kDa heat shock protein levels (Hsc/Hsp70). Feeding rate had species-specific effects on CTMax in green and white sturgeon such that CTMax of green sturgeon decreased as the magnitude of feed restriction increased; whereas, CTMax of white sturgeon did not change with feed restriction. Elevated temperature (28°C) and feed restriction increased Hsc/Hsp70 levels in the gill tissue of green sturgeon, while heat shock increased Hsc/Hsp70 levels in the mucus of white sturgeon. Our results suggest that green sturgeon may be more susceptible to temperature stress under food-limited conditions. PMID:27095630

  7. Ecological Requirements for Pallid Sturgeon Reproduction and Recruitment in the Lower Missouri River: A Research Synthesis 2005-08

    Science.gov (United States)

    DeLonay, Aaron J.; Jacobson, Robert B.; Papoulias, Diana M.; Simpkins, Darin G.; Wildhaber, Mark L.; Reuter, Joanna M.; Bonnot, Tom W.; Chojnacki, Kimberly A.; Korschgen, Carl E.; Mestl, Gerald E.; Mac, Michael J.

    2009-01-01

    This report provides a synthesis of results obtained between 2005 and 2008 from the Comprehensive Sturgeon Research Program, an interagency collaboration between the U.S. Geological Survey, Nebraska Game and Parks Commission, U.S. Fish and Wildlife Service, and the U.S. Army Corps of Engineers' Missouri River Recovery - Integrated Science Program. The goal of the Comprehensive Sturgeon Research Program is to improve fundamental understanding of reproductive ecology of the pallid sturgeon with the intent that improved understanding will inform river and species management decisions. Specific objectives include: *Determining movement, habitat-use, and reproductive behavior of pallid sturgeon; *Understanding reproductive physiology of pallid sturgeon and relations to environmental conditions; *Determining origin, transport, and fate of drifting pallid sturgeon larvae, and evaluating bottlenecks for recruitment of early life stages; *Quantifying availability and dynamics of aquatic habitats needed by pallid sturgeon for all life stages; and *Managing databases, integrating understanding, and publishing relevant information into the public domain. Management actions to increase reproductive success and survival of pallid sturgeon in the Lower Missouri River have been focused on flow regime, channel morphology, and propagation. Integration of 2005-08 Comprehensive Sturgeon Research Program research provides insight into linkages among flow regime, re-engineered channel morphology, and pallid sturgeon reproduction and survival. The research approach of the Comprehensive Sturgeon Research Program integrates opportunistic field studies, field-based experiments, and controlled laboratory studies. The field study plan is designed to explore the role of flow regime and associated environmental cues using two complementary approaches. An upstream-downstream approach compares sturgeon reproductive behavior between an upstream section of the Lower Missouri River with highly

  8. OBSERVATIONS REGARDING THE EMBRYONARY AND LARVAL DEVELOPMENT OF STERLET (ACIPENSER RUTHENUS

    Directory of Open Access Journals (Sweden)

    CRISTINA MUSCALU-NAGY

    2013-07-01

    Full Text Available Studying and presenting the embryo stages of sterlet has the main purpose of widening the knowledge horizon in this direction. Although all sterlet embryos originated from one female and from the same incubator (15°C, 9.6 mg/l of dissolved oxygen and a water flow of 2.7 l/min, they are in different stages of development at a certain time. Because the asynchronous development of embryos from one incubator is maintained throughout the incubation period, it is explained why not all embryos hatch at the same time. The first hatchings appear at the age of 145 hours from fecundation, the larvae have a very large yolk sack and are moving very fast, especially in obliquely upward direction. At one hour from hatching, the larvae presents clear opercularly movements. The last hatching occurs 193 hours from fecundation. The larval stage for Acipenser ruthenus lasts for 7-8 days, probably the larvae that hatched sooner have also a quicker absorption of yolk sack.

  9. 75 FR 53598 - Endangered and Threatened Wildlife and Plants; Threatened Status for Shovelnose Sturgeon Under...

    Science.gov (United States)

    2010-09-01

    ... huso) caviar into the United States (70 FR 57316, September 30, 2005; 70 FR 62135, October 28, 2005...) (16 U.S.C. 1531 et seq.) (55 FR 36641, September 6, 1990). The pallid sturgeon has a flattened, shovel... under the Act (55 FR 36641). At the time of listing, the primary threats and vulnerabilities for...

  10. A comparative study on biological aspect of sperm in sterlet and Siberian sturgeon

    Czech Academy of Sciences Publication Activity Database

    Pšenička, M.; Hadi Alavi, S.M.; Rodina, M.; Cosson, J.; Nebesářová, Jana; Gela, D.; Linhart, O.

    2008-01-01

    Roč. 32, č. 2 (2008), 179-180. ISSN 0399-0974 R&D Projects: GA ČR GA524/06/0817 Institutional research plan: CEZ:AV0Z60220518 Keywords : Sperm * Morphology * Physiology * Sterlet * Siberian sturgeon Subject RIV: EC - Immunology Impact factor: 0.403, year: 2008

  11. Fish and chips? Implanted transmitters help map the endangered pallid sturgeon

    Science.gov (United States)

    Chojnacki, Kimberly; DeLonay, Aaron

    2011-01-01

    With a flattened snout, long slender tail and rows of bony plates lining its body, the pallid sturgeon (Scaphirhynchus albus) has a unique, almost pre-historic, appearance. This endangered fish is native to the muddy, free-flowing waters of the Missouri River.

  12. Brain ventricle development in H. huso (Beluga sturgeon) larvae.

    Science.gov (United States)

    Tavighi, S H; Saadatfar, Z; Shojaei, B; Rassouli, M Behnam

    2016-09-01

    The development of ventricles in the brain of H. huso (Beluga sturgeon) from 1 to 54 days old is presented in this study. The components observed in the 1-day-old ventricular system were the telencephalic, tectal, and cerebellar ventricles. These ventricles were not observed to have any recess or sulcus. They were surrounded by copious ependymal and embryonic cells. Two different parts were detected in the 6-day-old telencephalic ventricle: the olfactory and lateral ventricle. The olfactory ventricle was observed as a cranial extension of the telencephalic ventricle from 6 days old, as was the inner cell layer of the olfactory bulb (ic) adjacent to this extension. In the preoptic region, the lateral ventricle was connected to the preoptic recess from 15 days old, and this recess was connected by the interventricular foramen to the third ventricle in the diencephalon. At 6 days old, the third ventricle in the diencephalon was visible at the caudal part of the lateral ventricle, and the third ventricle had a recess near to the inferior lobe of the hypothalamus. At 6 days old, the tectal ventricle was observed to have bilateral extensions which proceeded to grow with age. The cerebellar ventricle, situated between the two lobes of the cerebellum, was observed from 1 day old. The cerebellar ventricle grew with age, extending laterally from 6 days old. The connection of the cerebellar ventricle to the fourth ventricle in the medulla oblongata was visible from 6 days old. Upon dividing the ventricular system into three regions (forebrain, midbrain, and hindbrain), stereological studies performed utiizing Cavalieri's principle indicated that the forebrain ventricular region had the smallest volume while the hindbrain ventricular region had the largest. PMID:26530640

  13. Identifying structural elements needed for development of a predictive life-history model for pallid and shovelnose sturgeons

    Science.gov (United States)

    Wildhaber, Mark L.; DeLonay, A.J.; Papoulias, D.M.; Galat, D.L.; Jacobson, R.B.; Simpkins, D.G.; Braaten, P.J.; Korschgen, C.E.; Mac, M.J.

    2011-01-01

    Intensive management of the Missouri and Mississippi Rivers has resulted in dramatic changes to the river systems and their biota. These changes have been implicated in the decline of the pallid sturgeon (Scaphirhynchus albus), which has been listed as a United States federal endangered species. The sympatric shovelnose sturgeon (S. platorynchus) is more common and widespread but has also been in decline. The decline of pallid sturgeon is considered symptomatic of poor reproductive success and low or no recruitment. In order to organize information about these species and provide a basis for future development of a predictive model to help guide recovery efforts, we present an expert-vetted, conceptual life-history framework that incorporates the factors that affect reproduction, growth, and survival of shovelnose and pallid sturgeons.

  14. In situ evaluation of water quality and contaminants in the Lower Roanoke River : Survival and growth of shortnose sturgeon

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The aim of this study was to determine the suitability of present conditions in the lower Roanoke River for supporting shortnose sturgeon whether naturally...

  15. USE OF FEED YEAST IN FEEDING OF STURGEON (ACIPENSERINAE SPECIES (REVIEW

    Directory of Open Access Journals (Sweden)

    M. Simon

    2015-12-01

    Full Text Available Purpose. To review scientific sources on the use of feed yeast preparations in feeding of sturgeon species (Acipenserinae. Findings. The review of scientific works demonstrated that feed yeast in the feeding of sturgeons have been used as a source of vitamins and complete protein, the nutritional value of which is significantly higher than in the proteins of plant origin and are similar to the proteins of animal origin. In addition, a unit of yeast protein mass is significantly lower than in the feeds of animal origin. Moreover, based on the content of B group vitamins, feed yeast produced from the grain-potato spent wash exceed fish meal and meat-and-bone meal. The article highlights the peculiarities of the technological process of the production of different feed yeast species, amino acid and fatty acid composition of their preparations, basic physical and chemical parameters of their composition. The examples of feed yeast formulas for sturgeon species based on feed yeast preparations are presented. It was shown that sturgeon species, especially on early stages of their ontogenesis, could effectively use the feed yeast nucleotides. Thus, the latters can be an effective substitute of live zooplanktonic organisms. While the production of some feed yeast preparations (paprin, eprin was stopped in 1990s due to a number of social-economic reasons, the works on the creation of their full analogues was continued later. Currently, the trends of the development of world aquaculture anticipates the return to the use of yeast in fish feeding. Therefore, the interest of the agrarians of Ukraine in yeast lately increased and their use in agricultural sector increased by 2-2.5 times. Practical value. The array of the summarized information will be important for scientists who study the peculiarities of feeding of sturgeon species, because the data about the use of yeast as sources of complete protein in fish feeds is important in a constant search for the

  16. Hydraulic and Substrate Maps of Reaches Used by Sturgeon (Genus Scaphirhynchus) in the Lower Missouri River, 2005-07

    Science.gov (United States)

    Reuter, Joanna M.; Jacobson, Robert B.; Elliott, Caroline M.; Johnson, Harold E., III; DeLonay, Aaron J.

    2008-01-01

    This report is a repository of reach-scale maps of hydraulic and substrate characteristics generated for the habitat-use portion of an interdisciplinary sturgeon research project on the Lower Missouri River (from Gavins Point Dam to the junction with the Mississippi River). The maps were derived from hydroacoustic data sets that were collected for the purpose of assessing physical aquatic habitat in the vicinity of locations of adult shovelnose sturgeon (Scaphirhynchus platorynchus) and pallid sturgeon (S. albus). Hydroacoustic data sets were collected at the reach scale (mean reach length, 2.4 kilometers) in order to include the immediate vicinity of a targeted sturgeon location as well as the full range of habitat available at the bend and crossover scale. Reaches typically were surveyed on the day following the relocation of a telemetered sturgeon and at a discharge within 10 percent of the discharge on the sturgeon relocation date in order to characterize as closely as possible the channel morphology and flow-field conditions at the time that the sturgeon was present. One hundred fifty-three reaches were mapped during April-September in the years 2005 through 2007, with the majority of data collection occurring in the months of May and June (coinciding with the period of sturgeon migration and spawning in the Lower Missouri River). Interpolated maps (grid cell size, 5 meters) depict depth, generalized substrate, and depth-averaged velocity. Side-scan sonar imagery is also available for a subset of reaches. Collectively, the maps represent more than 20 percent of the length of the Lower Missouri River.

  17. Effects of feed restriction on the upper temperature tolerance and heat shock response in juvenile green and white sturgeon

    OpenAIRE

    Lee, S; Hung, SSO; Fangue, NA; Haller, L.; Verhille, CE; Zhao, J; Todgham, AE

    2016-01-01

    The objective of the current study was to investigate the effects of feed restriction on whole-organism upper thermal tolerance and the heat shock response of green and white sturgeon to determine how changes in food amount might influence physiological performance of each species when faced with temperature stress. Two parallel feed restriction trials were carried out for juvenile green (202g; 222-day post hatch: dph) and white sturgeon (205g; 197-dph) to manipulate nutritional status at 12....

  18. Ecological requirements for pallid sturgeon reproduction and recruitment in the Missouri River—A synthesis of science, 2005 to 2012

    Science.gov (United States)

    Delonay, Aaron J.; Chojnacki, Kimberly A.; Jacobson, Robert B.; Albers, Janice L.; Braaten, Patrick J.; Bulliner, Edward A.; Elliott, Caroline M.; Erwin, Susannah O.; Fuller, David B; Haas, Justin D.; Ladd, Hallie L.A.; Mestl, Gerald E.; Papoulias, Diana M.; Wildhaber, Mark L.

    2016-01-01

    This report is intended to synthesize the state of the scientific understanding of pallid sturgeon ecological requirements to provide recommendations for future science directions and context for Missouri River restoration and management decisions. Recruitment of pallid sturgeon has been low to non-existent throughout its range. Emerging understanding of the genetic structure of pallid sturgeon populations sets a broad framework for species and river management decisions, including decisions about managing the future genetic diversity of the species, but also decisions about where and what type of river restoration actions will be effective for subpopulations of this highly migratory species. Adult pallid sturgeon may migrate hundreds of kilometers (km) to spawn and their progeny may disperse even greater distances downstream as drifting free embryos. As a result of their complex life history pallid sturgeon naturally exploit a wide range of habitats during their life cycles. The construction of dams and reservoirs has fragmented habitats and may have shifted Missouri River subpopulations downstream. Research has not identified one primary biological or ecological constraint that appears to limit populations of the pallid sturgeon. With the present (2013) state of knowledge many life stages and life-stage transitions cannot be ruled out as contributing to recruitment failure.

  19. Columbia River White Sturgeon Genetics and Early Life History: Population Segregation and Juvenile Feeding Behavior, 1987 Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Brannon, Ernest L.

    1988-06-01

    The geographic area of the genetics study broadly covered the distribution range of sturgeon in the Columbia from below Bonneville Dam at Ilwaco at Lake Roosevelt, the Upper Snake River, and the Kootenai River. The two remote river sections provided data important for enhancement considerations. There was little electrophoretic variation seen among individuals from the Kootenai River. Upper Snake river sturgeon showed a higher percentage of polymorphic loci than the Kootenai fish, but lower than the other areas in the Columbia River we sampled. Sample size was increased in both Lake Roosevelt and at Electrophoretic variation was specific to an individual sampling area in several cases and this shaped our conclusions. The 1987 early life history studies concentrated on the feeding behavior of juvenile sturgeon. The chemostimulant components in prey attractive to sturgeon were examined, and the sensory systems utilized by foraging sturgeon were determined under different environmental conditions. These results were discussed with regard to the environmental changes that have occurred in the Columbia River. Under present river conditions, the feeding mechanism of sturgeon is more restricted to certain prey types, and their feeding range may be limited. In these situations, enhancement measures cannot be undertaken without consideration given to the introduction of food resources that will be readily available under present conditions. 89 refs., 7 figs., 11 tabs.

  20. Transcriptome Sequencing, De Novo Assembly and Differential Gene Expression Analysis of the Early Development of Acipenser baeri.

    Directory of Open Access Journals (Sweden)

    Wei Song

    Full Text Available The molecular mechanisms that drive the development of the endangered fossil fish species Acipenser baeri are difficult to study due to the lack of genomic data. Recent advances in sequencing technologies and the reducing cost of sequencing offer exclusive opportunities for exploring important molecular mechanisms underlying specific biological processes. This manuscript describes the large scale sequencing and analyses of mRNA from Acipenser baeri collected at five development time points using the Illumina Hiseq2000 platform. The sequencing reads were de novo assembled and clustered into 278167 unigenes, of which 57346 (20.62% had 45837 known homologues proteins in Uniprot protein databases while 11509 proteins matched with at least one sequence of assembled unigenes. The remaining 79.38% of unigenes could stand for non-coding unigenes or unigenes specific to A. baeri. A number of 43062 unigenes were annotated into functional categories via Gene Ontology (GO annotation whereas 29526 unigenes were associated with 329 pathways by mapping to KEGG database. Subsequently, 3479 differentially expressed genes were scanned within developmental stages and clustered into 50 gene expression profiles. Genes preferentially expressed at each stage were also identified. Through GO and KEGG pathway enrichment analysis, relevant physiological variations during the early development of A. baeri could be better cognized. Accordingly, the present study gives insights into the transcriptome profile of the early development of A. baeri, and the information contained in this large scale transcriptome will provide substantial references for A. baeri developmental biology and promote its aquaculture research.

  1. PECULIARITIES OF THE TRANSITION OF EARLY STURGEON (ACIPENSERIDAE FRY TO ARTIFICIAL FORMULATED FEEDS IN RAS (A REVIEW

    Directory of Open Access Journals (Sweden)

    M. Simon

    2016-03-01

    Full Text Available Purpose. To review scientific sources on the morphological and ecophysiological peculiarities of the transition of early sturgeon fry (Acipenseridae to artificial formulated feeds. To summarize the biotechnological fundamentals of the use of artificial formulated feeds in the conditions of recirculating aquaculture systems (RAS. Findings. The transition of early sturgeon fry to artificial formulated feeds is one of the most difficult stages of their rearing, even under controlled conditions of RAS. The review contains the description of the peculiarities of sturgeon embryogenesis, their behavior and morpho-physiological changes at this stage of their development. It contains main requirements for the rearing of sturgeon larvae in RAS. We showed that the ultimate refuse from natural (live or frozen forage organisms is not advisable; the optimum is their combination with artificial feeds with gradual predominance of the latters. We provided the schemes of their feeding based on the combination of natural and artificial feeds. We reviewed the most common biologically active supplements, which contributed to better feed digestion during the periods of the transition to exogenous feeding. We highlighted the effect of feeding with brine shrimp nauplii enriched with polyunsaturated fatty acids on the growth and development of early sturgeon fry. Practical value. The array of the summarized information will be important for scientists who study the peculiarities of the transition of early sturgeon fry to artificial formulated feeds in RAS. The data on the biotechnologies of rational feeding of early sturgeon fry in RAS in this period are important in the conditions of continuous search for the most effective replacement of live forage organisms and reduction of fish fry mortality in postembryogenesis.

  2. Effects of acclimation on poststocking dispersal and physiological condition of age-1 pallid sturgeon

    Science.gov (United States)

    Oldenburg, E.W.; Guy, C.S.; Cureton, E.S.; Webb, M.A.H.; Gardner, W.M.

    2011-01-01

    The objective of this study was to evaluate the effects of acclimation to flow and site-specific physicochemical water conditions on poststocking dispersal and physiological condition of age-1 hatchery-reared pallid sturgeon. Fish from three acclimation treatments were radio-tagged, released at two locations (Missouri River and Marias River), and monitored using passive telemetry stations. Marias treatment was acclimated to flow and site-specific physicochemical conditions, Bozeman treatment was acclimated to flow only, and controls had no acclimation (reared under traditional conservation propagation protocol). During both years, fish released in the Missouri River dispersed less than fish released in the Marias River. In 2005, Marias treatment dispersed less and nearly twice as many fish remained in the Missouri River reach as compared to control fish. In 2006, pallid sturgeon dispersed similarly among treatments and the number of fish remaining in the Missouri River reach was similar among all treatments. Differences in poststocking dispersal between years were related to fin curl which was present in all fish in 2005 and only 26% in 2006. Pallid sturgeon from all treatments in both years had a greater affinity for the lower reaches of the Missouri River than the upper reaches. Thus, release site influenced poststocking dispersal more than acclimation treatment. No difference was observed in relative growth rate among treatments. However, acclimation to flow (i.e., exercise conditioning) prevented fat accumulation from rupturing hepatocytes. Acclimation conditions used in this study did not benefit pallid sturgeon unless physiological maladies were present. Overriding all treatment effects was stocking location; thus, natural resource agencies need to consider stocking location carefully to reduce poststocking dispersal. ?? 2011 Blackwell Verlag, Berlin.

  3. Assessment of Lower Missouri River Physical Aquatic Habitat and Its Use by Adult Sturgeon (Genus Scaphirhynchus), 2005-07

    Science.gov (United States)

    Reuter, Joanna M.; Jacobson, Robert B.; Elliott, Caroline M.; DeLonay, Aaron J.

    2009-01-01

    This report presents an exploratory analysis of habitat availability and use by adult Scaphirhynchus sturgeon on the Lower Missouri River from Gavins Point Dam, South Dakota, to the junction with the Mississippi River. The analysis is based on two main data sources collected from 2005 to 2007: (1) a compilation of 153 reach-scale habitat maps (mean reach length, 2.4 kilometers) derived from boat-collected hydroacoustic data and (2) a sturgeon location dataset from which 378 sturgeon telemetry locations are associated with the maps (within 7 days of the mapping and within 10 percent of the discharge). The report focuses on: (1) longitudinal patterns of geomorphic and hydraulic characteristics revealed by the collection of reach maps; (2) assessment of environmental characteristics at sturgeon locations in the context of the mapped reaches; and (3) consideration of spatial distribution of habitat conditions that sturgeon appear to select. Longitudinal patterns of geomorphology, hydraulics, and associated habitats relate strongly to the engineered state of the river. Reaches within each of the following river sections tended to share similar geomorphic, hydrologic, and hydraulic characteristics: the Minimally Engineered section (Gavins Point Dam to Sioux City, Iowa), the Upstream Channelized section (Sioux City, Iowa, to the junction with the Kansas River), and the Downstream Channelized section (Kansas River to the junction with the Mississippi River). Adult sturgeon occupy nearly the full range of available values for each continuous variable assessed: depth, depth slope, depth-averaged velocity, velocity gradient, and Froude number (a dimensionless number relating velocity to depth). However, in the context of habitat available in a reach, sturgeon tend to select some areas over others. Reproductive female shovelnose sturgeon (Scaphirhynchus platorynchus), in particular, were often found in parts of the reach with one or more of the following characteristics: high

  4. Study on artificial hatching of sturgeon. Ovulation induction and artificial hatching of sturgeon; Chozame no jinko fuka ni kansuru kenkyu. Chozame (besuteru) no hairan yuhatsu to jinko fuka

    Energy Technology Data Exchange (ETDEWEB)

    Omoto, N.; Maebayashi, M. [Hokkaido Electric Power Co. Inc., Sapporo (Japan)

    1999-03-19

    With an objective to develop a technology serving for making Hokkaido more active industrially, a study has been carried out on culturing sturgeons by the biological environment group at the general research institute of Hokkaido Electric Power Company. The study has performed induction of ovulation and spermatozoon discharge by hormone treatment, artificial fecundation and hatching. In hormone treatment, emulstified LH-RHa was injected first into male individuals anticipated to have matured, and into female 24 hours later. Fecundation was performed by inseminating into roes the spermatozoon diluted by fresh water of volume as great as 100 times that of the spermatozoon. The result of this experiment revealed that artificial hatching is possible even under the frigid environment of Hokkaido by giving matured sturgeons a hormone treatment. However, such problems are still left that there are individuals that do not ovulate even the hormone is given, and hatching rate of obtained roes is low. The important point in the hormone treatment technology is in selection of matured parent fishes. Regarding female individuals, a more efficient and precise maturity determination method is desired than the method to observe gonads by biopsy. (NEDO)

  5. Analysis of piscicultural-biological results of works with Russian sturgeon brood fish at the sturgeon hatchery “Lebyazhy” (Astrakhan region, Russia

    Directory of Open Access Journals (Sweden)

    R. V. Kononenko

    2013-04-01

    Full Text Available The state of world stocks of sturgeons is on the edge of catastrophe. These species are either extinct or under threat of extinction under human impacts. At the same time, there are enterprises, fish hatcheries, which deal with restoration and replenishment of natural stocks with of endangered fish species. One of such hatcheries is the sturgeon hatchery “Lebyazhy” (Astrakhan region, Russian Federation. The aim of the study was an analysis of piscicultural-biological features of the Russian sturgeon brood fish. During the study, which was conducted in April–May 2011, 34 Russian sturgeon females were used in two rounds, 17 individuals each. For stimulating gametes maturation, the Derzhavin’s physiological method was used. Caviar was obtained by stripping the eggs under strict hygienic and sanitary norms. Eggs fertilization with the semi-dry method used the male milt that bought at the “Raskat” LLC. Egg stickiness elimination was performed with the aid of talc and apparatuses for the egg stickiness elimination. Eggs incubation was performed in the “Osetr” apparatuses until yolk-sac larvae hatching. The domesticated fish were subjected to bonitation for determining their readiness for spawning. As a result of this bonitation, the brood fish were separated into two groups: first round of rearing works: females with mean weight of 34.8 kg and age of 9 years; second round: females with mean weight of 32.3 kg and the same age. Among injected females of the first round, 100% positive reaction for the stimulating injection was observed, but 95% – among females of the second round. Maturation time of females of both rounds varied from 25 to 30 hours. The maturation state of gametes of sturgeon females or males was determined based on samples obtained. 90.2 kg of eggs were obtained from females of the first round. At the same time, the maximum quantity was observed in the female of 50.5 kg – 9.2 kg of caviar, and the least quantity

  6. White Sturgeon Mitgation and Restoration in the Columbia and Snake Rivers Upstream from Bonneville Dam; 2003-2004 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Rein, Thomas A.; Hughes, Michele L.; Kern, J. Chris (Oregon Department of Fish and Wildlife, Clackamas, OR)

    2005-08-01

    We report on our progress from April 2003 through March 2004 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam. This is a multi-year study with many objectives requiring more than one year to complete; therefore, findings from a given year may be part of more significant findings yet to be reported.

  7. White Sturgeon Mitigation and Restoration in the Columbia and Snake Rivers Upstream from Bonneville Dam; 2004-2005 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Rien, Thomas A.; Hughes, Michele L.; Kern, J. Chris (Oregon Department of Fish and Wildlife, Clackamas, OR)

    2006-03-01

    We report on our progress from April 2004 through March 2005 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam. This is a multi-year study with many objectives requiring more than one year to complete; therefore, findings from a given year may be part of more significant findings yet to be reported.

  8. Effects of Mitigative Measures on Productivity of White Sturgeon Populations in the Columbia River Downstream from McNary Dam; Determine Status and Habitat Requirements of White Sturgeon Populations in the Columbia and Snake Rivers Upstream from the McNary Dam, 1994-1995 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Beiningen, Kirk T. [Oregon Dept. of Fish and Wildlife, Portland, OR (US)

    1996-03-01

    The author reports on progress from April 1994 through March 1995 of research on white sturgeon in the lower Columbia River. The study began in July 1986 and is a cooperative effort of federal, state and tribal fisheries entities to determine the (1) the status and habitat requirements, and (2) the effects of mitigative measures on productivity of white sturgeon populations in the lower Columbia River. This report describes activities conducted during the third year of this contract's second phase. Information was collected, analyzed, and evaluated on sub-adult and adult life histories, population dynamics, quantity and quality of habitat, and production enhancement strategies. The report is divided into sections that evaluate success of developing and implementing a management plan for white sturgeon; evaluate growth, mortality, and contributions to fisheries of juvenile white sturgeon transplanted from areas downstream; describe the life history and population dynamics of sub-adult a nd adult white sturgeon; define habitat requirements for spawning and rearing of white sturgeon and quantify the extent of habitat available; describe reproductive and early life history characteristics of white sturgeon; and quantify physical habitat used by spawning and rearing white sturgeon in the free-flowing portion of the Columbia River.

  9. Effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and determine status and habitat requirements of white sturgeon populations in the Columbia and Snake Rivers upstream from the McNary Dam. Annual progress report, April 1994--March 1995

    International Nuclear Information System (INIS)

    The author reports on progress from April 1994 through March 1994 of research on white sturgeon in the lower Columbia River. The study began in July 1986 and is a cooperative effort of federal, state and tribal fisheries entities to determine the (1) the status and habitat requirements, and (2) the effects of mitigative measures on productivity of white sturgeon populations in the lower Columbia River. This report describes activities conducted during the third year of this contract's second phase. Information was collected, analyzed, and evaluated on subadult and adult life histories, population dynamics, quantity and quality of habitat, and production enhancement strategies. The report is divided into sections that evaluate success of developing and implementing a management plan for white sturgeon; evaluate growth, mortality, and contributions to fisheries of juvenile white sturgeon transplanted from areas downstream; describe the life history and population dynamics of subadult and adult white sturgeon; define habitat requirements for spawning and rearing of white sturgeon and quantify the extent of habitat available; describe reproductive and early life history characteristics of white sturgeon; and quantify physical habitat used by spawning and rearing white sturgeon in the free-flowing portion of the Columbia River

  10. Hematocrit and plasma osmolality values of young-of-year shortnose sturgeon following acute exposures to combinations of salinity and temperature

    Science.gov (United States)

    Ziegeweid, J.R.; Black, M.C.

    2010-01-01

    Little is known about the physiological capabilities of young-of-year (YOY) shortnose sturgeon. In this study, plasma osmolality and hematocrit values were measured for YOY shortnose sturgeon following 48-h exposures to 12 different combinations of salinity and temperature. Hematocrit levels varied significantly with temperature and age, and plasma osmolalities varied significantly with salinity and age. Plasma osmolality and hematocrit values were similar to previously published values for other sturgeons of similar age and size in similar treatment conditions. ?? 2010 Springer Science+Business Media B.V.

  11. Review of BPA Funded Sturgeon, Resident Fish and Wildlife Projects for 1990/1991.

    Energy Technology Data Exchange (ETDEWEB)

    United States. Bonneville Power Administration.

    1990-12-01

    The Bonneville Power Administration (BPA) held a public meeting on November 19--21, 1991, for the purpose of review, coordination, and consultation of the BPA-funded projects for sturgeon, resident fish, and wildlife in the Columbia River Basin (Basin). The comments received after the meeting were favorable and the participants agreed that the meeting was stimulating and productive. The information exchanged should lead to better coordination with other projects throughout the Basin. This document list the projects by title, the project leaders and BPA's project officers, and an abstract of each leader's presentation.

  12. Vegetable Production in an Integrated Aquaponic System with Stellate Sturgeon and Spinach – Matador variety

    OpenAIRE

    Stefan Mihai Petrea; Victor Cristea; Lorena Dediu; Maria Contoman; Maria Dicu; Alina Antache; Marian Coada; Sandita Placinta

    2014-01-01

    This study aims to reveal the performances parameters, both in terms of quantity and quality, for spinach (Spinacia oleracea) - Matador variety, growth in an aquaponic integrated system, along with stellate sturgeons (A. stellatus) under three crops densities (V1 - 59 crops/m2, V2 – 48 crops/m2 and V3 – 39 crops/m2), by using hydroton as growing substrate, under a continuous flow hydraulic regime. The experiment was run in triplicate for each one of the three variants. The water quality was m...

  13. Increasing capture efficiency of pallid sturgeon Scaphirhynchus albus (Forbes and Richardson, 1905) and the reliability of catch rate estimates

    Science.gov (United States)

    DeVries, R. J.; Hann, D. A.; Schramm, H.L., Jr.

    2015-01-01

    This study evaluated the effects of environmental parameters on the probability of capturing endangered pallid sturgeon (Scaphirhynchus albus) using trotlines in the lower Mississippi River. Pallid sturgeon were sampled by trotlines year round from 2008 to 2011. A logistic regression model indicated water temperature (T; P < 0.01) and depth (D; P = 0.03) had significant effects on capture probability (Y = −1.75 − 0.06T + 0.10D). Habitat type, surface current velocity, river stage, stage change and non-sturgeon bycatch were not significant predictors (P = 0.26–0.63). Although pallid sturgeon were caught throughout the year, the model predicted that sampling should focus on times when the water temperature is less than 12°C and in deeper water to maximize capture probability; these water temperature conditions commonly occur during November to March in the lower Mississippi River. Further, the significant effect of water temperature which varies widely over time, as well as water depth indicate that any efforts to use the catch rate to infer population trends will require the consideration of temperature and depth in standardized sampling efforts or adjustment of estimates.

  14. 75 FR 2102 - Endangered and Threatened Wildlife and Plants; Proposed Rule To List the Shovelnose Sturgeon as...

    Science.gov (United States)

    2010-01-14

    ... week. SUPPLEMENTARY INFORMATION: Background On September 22, 2009, we published a proposed rule (74 FR..., 2009 proposal required written requests for a public hearing to be submitted by November 6, 2009 (74 FR... fishing of shovelnose sturgeon, which is in demand for its roe (eggs sold as caviar). The...

  15. The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity

    Czech Academy of Sciences Publication Activity Database

    Havelka, M.; Bytyutskyy, D.; Symonová, R.; Flajšhans, M.

    2016-01-01

    Roč. 48, č. 12 (2016). ISSN 0999-193X R&D Projects: GA ČR GA14-02940S Institutional support: RVO:67985904 Keywords : sturgeon * spontaneous autopolyploidy * image-analysis densitometry Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.821, year: 2014

  16. Kootenai River White Sturgeon Recovery Implementation Plan and Schedule; 2005-2010, Technical Report 2004-2005.

    Energy Technology Data Exchange (ETDEWEB)

    Anders, Paul

    2007-03-01

    Kootenai River white sturgeon have been declining for at least 50 years and extinction of the wild population is now imminent (Paragamian et al. 2005). Only 630 adults were estimated to remain in 2002 from a population ten times that size just 20 years ago. Significant recruitment of young sturgeon has not been observed since the early 1970s and consistent annual recruitment has not been seen since the 1950s. The remaining wild population consists of a cohort of large, old fish that is declining by about 9% per year as fish die naturally and are not replaced. At this rate, the wild population will disappear around the year 2040. Numbers have already reached critical low levels where genetic and demographic risks are acute. The Kootenai River White Sturgeon Recovery Team was convened in 1994, provided a draft Recovery Plan in 1996 and the first complete Recovery Plan for Kootenai River white sturgeon in 1999 (USFWS 1996, 1999). The Plan outlined a four part strategy for recovery, including: (1) measures to restore natural recruitment, (2) use of conservation aquaculture to prevent extinction, (3) monitoring survival and recovery, and (4) updating and revising recovery plan criteria and objectives as new information becomes available. Sturgeon recovery efforts are occurring against a backdrop of a broader ecosystem protection and restoration program for the Kootenai River ecosystem. With abundance halving time of approximately 8 years, the Kootenai River white sturgeon population is rapidly dwindling, leaving managers little time to act. Decades of study consistently indicate that recruitment failure occurs between embryo and larval stages. This assertion is based on four key observations. First, almost no recruitment has occurred during the last 30 years. Second, thousands of naturally produced white sturgeon embryos, most viable, have been collected over the past decade, resulting from an estimated 9 to 20 spawning events each year. Third, Kootenai River white

  17. Chemical and biochemical composition of caviar from different sturgeon species and origins.

    Science.gov (United States)

    Wirth, M; Kirschbaum, F; Gessner, J; Krüger, A; Patriche, N; Billard, R

    2000-08-01

    The chemical and biochemical composition of caviar in 22 specimens of wild caught and of 2 farmed animals were measured. The results include grain size, protein and fat content, fatty acid composition of triglycerides and phospholipids, as well as the concentrations of relevant heavy metals and chlorinated hydrocarbons. The average protein content varied between 26.2 and 31.1% (wet weight) and fat from 10.9 to 19.4% (wet weight) with lowest values for caviar from farmed sturgeon. The triglycerides and phospholipids contained more n-3 fatty acids, especially eicosapentaenoic and docosahexaenoic acid than n-6 fatty acids. The copper and zinc concentrations varied between 1.20 and 1.69 and 10.3 and 12.4 mg/kg (wet weight), respectively. These values reflect the elevated requirement of sturgeons for these components. Lead content varied between 0.06 and 0.15 mg/kg (wet weight). The cadmium concentrations were less than 5 micrograms/kg (wet weight) leading to the conclusion that no accumulation took place in the eggs. The concentrations of sigma DDT and sigma PCB were extremely high in caviar from Huso huso compared to the samples of the other species thus reflecting the different food habits leading to increased bio-accumulation. PMID:10996895

  18. Metal content in caviar of wild Persian sturgeon from the southern Caspian Sea.

    Science.gov (United States)

    Hosseini, S M; Sobhanardakani, S; Navaei, M Batebi; Kariminasab, M; Aghilinejad, S M; Regenstein, J M

    2013-08-01

    Caviar (fish roe of sturgeon) may contain high levels of contaminants. An inductively coupled plasma-optical emission spectrometer and a direct mercury analyzer were used to assess the contents of four heavy metals (Hg, Se, Sn, and Ba) in caviar of wild Persian sturgeon sea foods. The levels of Hg ranged from 1.39 to 1.50 μg g(-1), Se from 0.90 to 1.10 μg g(-1), Sn from 0.23 to 0.33, and Ba from 0.71 to 1.17 µg g(-1). Evaluation of these levels showed that except for Hg, the average concentrations of other metals are significantly lower than adverse level for the human consumption when compared with Food and Agricultural Organization of the United Nations and World Health Organization permissible limits. Therefore, their contribution to the total body burden of these heavy metals can be considered as negligibly small given that caviar is a luxury product. PMID:23504250

  19. Heavy metal bioaccumulation and risk assessment for wild and farmed beluga sturgeon caviar.

    Science.gov (United States)

    Hosseini, Seyed Vali; Hosseini, Seyed Mehdi; Monsef Rad, Seyede Fatemeh; Mobinifar, Mohammad; Regenstein, Joe M

    2013-12-01

    Because of over-exploitation of sturgeon for caviar production, they have been listed worldwide in annex II of the Convention on International Trade in Endangered Species regulations; so caviar production using aquaculture is increasingly seen as a feasible way to reduce overfishing. The accumulation of the nonessential metals As, Ba, Cd, Hg, Pb, and Sn was determined in the caviar of farmed and wild Beluga sturgeon (Huso huso). The levels of As in both and Cd in wild samples were less than 0.01 mg kg(-1) wet weight, and the comparison for all of the metals studied did not show large fluctuations in metal concentrations between farmed and wild caviar samples. The average for each toxic metal was below the permissible limits proposed by the UK's Ministry of Agriculture, Fisheries and Foods (2000). The maximum allowable daily consumption rate of caviar was calculated; however, the health risks from caviar consumption are uncertain because the amount of caviar consumed by heavy users is not known. PMID:23793540

  20. Effects of Exposure to the Sound from Seismic Airguns on Pallid Sturgeon and Paddlefish.

    Science.gov (United States)

    Popper, Arthur N; Gross, Jackson A; Carlson, Thomas J; Skalski, John; Young, John V; Hawkins, Anthony D; Zeddies, David

    2016-01-01

    This study examined the effects of exposure to a single acoustic pulse from a seismic airgun array on caged endangered pallid sturgeon (Scaphirhynchus albus) and on paddlefish (Polyodon spathula) in Lake Sakakawea (North Dakota, USA). The experiment was designed to detect the onset of physiological responses including minor to mortal injuries. Experimental fish were held in cages as close as 1 to 3 m from the guns where peak negative sound pressure levels (Peak- SPL) reached 231 dB re 1 μPa (205 dB re 1 μPa2·s sound exposure level [SEL]). Additional cages were placed at greater distances in an attempt to develop a dose-response relationship. Treatment and control fish were then monitored for seven days, euthanized, and necropsied to determine injuries. Necropsy results indicated that the probability of delayed mortality associated with pulse pressure following the seven day monitoring period was the same for exposed and control fish of both species. Exposure to a single pulse from a small air gun array (10,160 cm3) was not lethal for pallid sturgeon and paddlefish. However, the risks from exposure to multiple sounds and to sound exposure levels that exceed those reported here remain to be examined. PMID:27505029

  1. Hierarchical Bayesian Markov switching models with application to predicting spawning success of shovelnose sturgeon

    Science.gov (United States)

    Holan, S.H.; Davis, G.M.; Wildhaber, M.L.; DeLonay, A.J.; Papoulias, D.M.

    2009-01-01

    The timing of spawning in fish is tightly linked to environmental factors; however, these factors are not very well understood for many species. Specifically, little information is available to guide recruitment efforts for endangered species such as the sturgeon. Therefore, we propose a Bayesian hierarchical model for predicting the success of spawning of the shovelnose sturgeon which uses both biological and behavioural (longitudinal) data. In particular, we use data that were produced from a tracking study that was conducted in the Lower Missouri River. The data that were produced from this study consist of biological variables associated with readiness to spawn along with longitudinal behavioural data collected by using telemetry and archival data storage tags. These high frequency data are complex both biologically and in the underlying behavioural process. To accommodate such complexity we developed a hierarchical linear regression model that uses an eigenvalue predictor, derived from the transition probability matrix of a two-state Markov switching model with generalized auto-regressive conditional heteroscedastic dynamics. Finally, to minimize the computational burden that is associated with estimation of this model, a parallel computing approach is proposed. ?? Journal compilation 2009 Royal Statistical Society.

  2. Vegetable Production in an Integrated Aquaponic System with Stellate Sturgeon and Spinach – Matador variety

    Directory of Open Access Journals (Sweden)

    Stefan Mihai Petrea

    2014-05-01

    Full Text Available This study aims to reveal the performances parameters, both in terms of quantity and quality, for spinach (Spinacia oleracea - Matador variety, growth in an aquaponic integrated system, along with stellate sturgeons (A. stellatus under three crops densities (V1 - 59 crops/m2, V2 – 48 crops/m2 and V3 – 39 crops/m2, by using hydroton as growing substrate, under a continuous flow hydraulic regime. The experiment was run in triplicate for each one of the three variants. The water quality was monitored and a series of growth parameters were determined, as follows: leaf area index (LAI, relative growth rate (RGR, average net assimilation rate (NAR, mean leaf area ratio (LAR and crop growth rate (CGR. Also the concentration of chlorophyll a, b, carotenoids, ash and dry matter for spinach leaf, from each of the three experimental variants was determined and compared with the one of marketable spinach, growth conventional, in soil. It can be concluded that statistical significant differences (p<0.05 were recorded in terms of growth performance and crops quality, between the experimental variants. Also the quality of spinach grown in aquaponic conditions, by using effluent derived from stellate sturgeon intensive aquaculture is similar to that of the marketable spinach, growth conventional.

  3. Status and Habitat Requirements of White Sturgeon Populations in the Columbia River Downstream from McNary Dam, 1988-1989 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Nigro, Anthony A. (Oregon Dept. of Fish and Wildlife, Portland, OR (USA))

    1989-09-01

    We report on our progress from April 1988 through March 1989 on determining the status and habitat requirements of white sturgeon populations in the Columbia River downstream from McNary Dam. Highlights of results of our work in the Dalles and Bonneville reservoirs are: using setlines, we caught 1,586 sturgeon in The Dalles Reservoir and 484 sturgeon in Bonneville Reservoir in 1988. Fork length of fish caught ranged from 34 cm to 274 cm. Of the fish caught we marked 1,248 in The Dalles Reservoir and 341 in Bonneville Reservoir. Of the fish marked in 1988, we recaptured 82 in The Dalles Reservoir and none in Bonneville Reservoir. We recaptured 89 fish marked in 1987 in The Dalles Reservoir. Anglers recaptured 35 fish marked in 1988 and 16 fish marked in 1987 in The Dalles Reservoir. Anglers recaptured 2 sturgeon marked in 1988 in Bonneville Reservoir. Individual papers were processed separately for the data base.

  4. Monitoring the effects of storage in caviar from farmed Acipenser transmontanus using chemical, SEM, and NMR methods.

    Science.gov (United States)

    Gussoni, Maristella; Greco, Fulvia; Vezzoli, Alessandra; Paleari, Maria Antonietta; Moretti, Vittorio Maria; Beretta, Giuseppe; Caprino, Fabio; Lanza, Barbara; Zetta, Lucia

    2006-09-01

    The effects of storage at 4 degrees C on the quantity and quality of chemical components in the caviar from farmed Acipenser transmontanus have been analyzed by SEM, chemical methods, and NMR and MRI techniques. Particular attention has been focused on the lipid components, the distribution and mobility of which were strongly affected by the storage time. MRI and relaxation data indicated that lipids are endowed with two different mobility regimes, one slow (short T1) and one fast (long T1), both lengthening with the storage time. Chemical analysis assessed a total fat content that remained practically unchanged and a constant fatty acid composition during the total storage time. The combination of the two methods allowed one (a) to suppose that a mechanism of lipid hydrolysis (faster in unsalted than in salted eggs) is still occurring during storage of caviar at 4 degrees C for up to approximately 4 months and (b) to exclude that an intensive oxidative process is active in the same storage period. PMID:16939332

  5. Preliminary Results about Growth Performance and Food Conversion Ratio of Acipenser stellatus juvenils, Fed with Different Dietary Protein Levels

    Directory of Open Access Journals (Sweden)

    Maria Desimira Dicu

    2012-10-01

    Full Text Available The main purpose of this study is to evaluate the effects of different dietary protein levels on Acipenser stellatus growth performance. Researches were conducted between 4 th November-3rd December 2011, in the recirculating system of Aquaculture, Environmental Science and Cadastre Department, University of Galati. A number of 60 fish with an average weight of 11.5±0.58 g were randomly stocked into four rearing units, with same stocking densities. Two experimental variants with different dietary protein levels, 64% (V1 respectively 41% (V2, were applied in duplicate. At the end of the experiment, the specific growth rate (SGR, calculated as mean value of both repetitions for each variant, was 4,64%/day at V1 and 4,63%/day at V2. Protein efficiency ratio PER (g/g registered a better value at V2 (2.55 g/g, comparing to V1 (1.62 g/g. Regarding feed conversion ratio, almost identical values were observed in case of both experimental variants: 0,96 at V1 and 0.97 at V2. The final results of the experiment showed that while PER was significantly influenced by dietary protein levels, the rest of growing indicators had a very limited variation scale.

  6. Metric Characters and Body Indices of Juvenile Sterlet Acipenser ruthenus Raised in a Recirculating Aquaculture System (RAS

    Directory of Open Access Journals (Sweden)

    Mărioara Nicula

    2015-05-01

    Full Text Available Our research was performed on juvenile sterlets (Acipenser ruthenus, aged between 346-431 days, maintained in five round pools (Ø=3 m, V=6 m3, h-100 cm and fed with a commercial extruded pellet (3 mm granulation, 48% CP, and 10% EE. Body weight and total body length on 30 individuals from each tank were determined every two weeks. Weighing and somatic measurements achieved at the end of experimental period (standard length-l, body weight-G, maximum body high–H, maximum body perimeter (Pmax, head length–lh,, caudal peduncle length-lp allowed us to calculate the following body indices: profile index, thickness index, Fulton index, Kiselev index, meat index. Statistical analysis was performed by using the SPPS IBM 22 and Minitab 16 software. Data were reported as Mean±Sx at a significant level of p<0.05. Testing differences between means was realized by ANOVA completed with post-hoc Tukey test. Results obtained led to conclude that specimens analyzed had a good maintenance and feeding, an adequate development, a high proportion of meat, an elongated body and a straight back, and they are eligible for selection.

  7. Chinese Cooking.

    Science.gov (United States)

    Kane, Tony

    This unit, intended for secondary level students, is a general introduction to Chinese cooking. It is meant to inform students about the origins of Chinese cooking styles in their various regional manifestations, and it can be used to discuss how and why different cultures develop different styles of cooking. The first part of the unit, adapted…

  8. 中华鲟铁蛋白基因cDNA全长克隆与组织表达分析%Full-length cDNA Cloning and Tissues Expression Analysis of Ferritin Gene from Acipenser sinensis

    Institute of Scientific and Technical Information of China (English)

    陈晓武; 施志仪; 程千千

    2009-01-01

    根据铁蛋白基因的保守序列,搜索GenBank数据库中华鲟的EST数据库得到一条同源序列.通过RT-PCR的方法对该序列进行扩增,修改其测序错误,获得中华鲟铁蛋白亚基cDNA全长,经过注释提交GenBank数据库,获取序列登录号EU348782.该cDNA长度为896bp,包含531bp的完整编码区,推测编码的蛋白质为176aa,分子量为20339.9Mr,理论等电点为5.66.它和大两洋鲑鱼铁蛋白序列同源性最高,达到82.9%.该基闪在中华鲟肝脏、胰脏、肌肉、脑、心脏、鳃和胃粘膜等多种组织表达,在胰脏和心脏中表达量较高,在肌肉组织中表达较低.根据同源模建的方法得到该蛋白质三维结构,其包括5个α螺旋和10个转角结构,和人、蛙和细菌的铁蛋白均能很好的叠合,表现了很高的相似性,表明该蛋白结构和功能在基因进化中的高度保守性.%According to the conserved sequence of the ferritin gene, a homologous sequence was obtained from the EST database through a BLAST search against the GenBank database. This sequence was amplified with the method of RT-PCR, false sequencing was corrected, and full length cDNA of the ferritin subunit from the Chinese sturgeon was obtained. After being submitted to the GenBank database, the sequence accession number EU348782 was assigned. With the length of 896 bp, this cDNA includes entire coding regions of 531 bp, which encodes 176 amino acids (aa). The molecular weight was predicted to be 20339.9Mr and the theoretical isoelectrie point 5.66. It shares 82.9% protein sequence homology with the ferritin of the Atlantic salmon. This gene is expressed in many organs of the Chinese sturgeon, for example, the liver, pancreas, muscle, brain, heart and gastric mucosa. The highest expression level was found in the pancreas and the heart, while the muscular tissue showed the lowest. Homology modeling was used to predict the 3-D structure of the protein, which included 5 alpha helices and 10 turns

  9. Assessment of essential elements in the wild Beluga Sturgeon (Huso huso caviar from Caspian Sea

    Directory of Open Access Journals (Sweden)

    Seyed Vali Hosseini

    2014-12-01

    Full Text Available In this study, the concentration of Calcium (Ca, Cobalt (Co, Chromium (Cr, Copper (Cu, Iron (Fe, Potassium (K, Magnesium (Mg, Manganese (Mn, Selenium (Se and Zinc (Zn as essential metals, were determined in caviar of wild beluga sturgeon caught from the Caspian Sea in March 2012. Potassium (4885.51± 17.81 µg g-1 and Magnesium (346.61± 6.6 µg g-1 had the highest concentration and Cobalt and Manganese levels were less than 0.01 mg kg-1 wet weight. The mean concentration of Copper and Zinc in the caviar samples were under the permissible limits proposed by the UK’s Ministry of Agriculture, Fisheries and Foods. The caviar maximum allowable daily consumption rate was calculated. However, the health risks from caviar consumption are uncertain because the amount of caviar consumed by heavy users is not known.

  10. Research regarding the evolution of corporal masses and feed conversion on sterlet (Acipenser ruthenus juvenile raised in recirculating system

    Directory of Open Access Journals (Sweden)

    Marian Bura

    2009-04-01

    Full Text Available The research was performed on 3375 specimens of sterlet juveniles (Acipenser ruthenus maintained in five square pools (1.5 x 1.5 x 0.7 m; V=1.125 m3 and in a round pool (Ø=3 m; H=1 m; V=5.63 m3. Water temperature varied between 14-16°C. Sterlet juvenile was fed with Dana Feed forage granulation 1.5 mm (52% raw protein and 13% raw fat. Body weight and body length on 30 individuals from each tank were determined every two weeks. Based on weighings of body mass and quantity of  feed given calculations, it had been established bioproductive indicators at juvenile sterlets aged between 135-178 days. At the age of 178 days, the sterlet juvenile reached an average body mass that ranged between 56.60±2.19 g and 78.50±2.07 g, and an average body length that ranged between 23.83±0.29 cm and 27.22±0.23 cm. Individual values of minimum and maximum body mass were located between 30 g and 104 g and body length between 19 and 30 cm. During the 43 experimental days, the 3375 juvenile sterlets have acquired a real weight gain of 142.91 kg, with a specific consumption of feed of 0.79 kg feed/kg spore and an index of feed conversion of 1.27 kg spore/kg feed consumed.

  11. Chinese astronomy

    OpenAIRE

    Macfarlane, Alan; Cullen, Christopher

    2004-01-01

    Standing in the observatory in Beijing, Christopher Cullen discusses the nature and sophistication of Chinese astronomy in the medieval period. The political as well as the intellectual interest in astronomy is outlined.

  12. Chinese Confucianism

    OpenAIRE

    Macfarlane, Alan; Cullen, Christopher

    2004-01-01

    Confucianism has deeply influenced Chinese civilization. Christopher Cullen describes its effect on education, social structure and knowledge over the past centuries, against the backdrop of a Confucian building in Beijing.

  13. Contributions to understanding the fall migration of beluga sturgeon (Huso huso on the Lower Danube River, Romania

    Directory of Open Access Journals (Sweden)

    Alin M. Bâdiliţă

    2013-07-01

    Full Text Available Within this article the authors present the processing of an informational volume, unique on bothnational and international plan, referring to sturgeons’ migration on Danube. In order to present the results ofthe monitoring activity, carried out by a researching mix team of the above mentioned institutes, we chose themarine sturgeon beluga (Huso huso. The objective is to offer exclusive results regarding the migration of thissturgeon species in order to elaborate some protection and conservation measures in the future, taking intoconsideration the fact that this species is mentioned starting April the 1st 1998 in the 2nd axis of The Conventionof International Trade in Endangered Species of Wild Fauna and Flora. This paper is based on Lower Danube’smonitoring section, covered between km 175-375, using specific techniques (DKTB station to monitor theichtyofauna, especially sturgeons, through remote sensing, with ultrasonic tags in different hydromorphologicalconditions, file number: no. A100773/30.10.2012. The article presents the results that come to improve theexistent informational volume throughout the identification of some migrating routes of the sturgeon (belugaspecies directly correlated to water temperature, swimming depth and geodesic localization as well asindirectly with the average velocity of movement. The exceptional character of this paper consists in the factthat until now, in Romania, there has not been possible the accomplishment of sturgeons’ migration monitoringon a significant number of individuals and on a distance of more than 400 km, using ultrasonic tags.

  14. 2006 Annual Synthesis Report, Pallid Sturgeon Population Assessment Program and Associated Fish Community Monitoring for the Missouri River

    Energy Technology Data Exchange (ETDEWEB)

    Oldenburg, Eric W.; Hanrahan, Timothy P.; Harnish, Ryan A.; Bellgraph, Brian J.; Duncan, Joanne P.; Allwardt, Craig H.

    2008-08-12

    Pallid sturgeon, Scaphirhynchus albus, have declined throughout the Missouri River since dam construction and inception of the Bank Stabilization and Navigation Project in 1912. Their decline likely is due to the loss and degradation of their natural habitat as a result of changes in the river’s structure and function, as well as the pallid sturgeon’s inability to adapt to these changes. The U. S. Army Corps of Engineers has been working with state and federal agencies to develop and conduct a Pallid Sturgeon Monitoring and Assessment Program (Program), with the goal of recovering pallid sturgeon populations. The Program has organized the monitoring and assessment efforts into distinct geographic segments, with state and federal resource management agencies possessing primary responsibility for one or more segment. To date, the results from annual monitoring have been reported for individual Program segments. However, monitoring results have not been summarized or evaluated for larger spatial scales, encompassing more than one Program segment. This report describes a summary conducted by the Pacific Northwest National Laboratory (PNNL) that synthesizes the 2006 sampling year monitoring results from individual segments.

  15. 2005 Annual Synthesis Report, Pallid Sturgeon Population Assessment Program and Associated Fish Community Monitoring for the Missouri River

    Energy Technology Data Exchange (ETDEWEB)

    Oldenburg, Eric W.; Hanrahan, Timothy P.; Harnish, Ryan A.; Bellgraph, Brian J.; Duncan, Joanne P.; Allwardt, Craig H.

    2008-08-12

    Pallid sturgeon, Scaphirhynchus albus, have declined throughout the Missouri River since dam construction and inception of the Bank Stabilization and Navigation Project in 1912. Their decline likely is due to the loss and degradation of their natural habitat as a result of changes in the river’s structure and function, as well as the pallid sturgeon’s inability to adapt to these changes. The U. S. Army Corps of Engineers has been working with state and federal agencies to develop and conduct a Pallid Sturgeon Monitoring and Assessment Program (Program), with the goal of recovering pallid sturgeon populations. The Program has organized the monitoring and assessment efforts into distinct geographic segments, with state and federal resource management agencies possessing primary responsibility for one or more segment. To date, the results from annual monitoring have been reported for individual Program segments. However, monitoring results have not been summarized or evaluated for larger spatial scales, encompassing more than one Program segment. This report describes a summary conducted by the Pacific Northwest National Laboratory (PNNL) that synthesizes the 2005 sampling year monitoring results from individual segments.

  16. Remote-sensing of Riverine Environments Utilized by Spawning Pallid Sturgeon Using a Suite of Hydroacoustic Tools and High-resolution DEMs

    Science.gov (United States)

    Elliott, C. M.; Jacobson, R. B.; DeLonay, A. J.; Braaten, P. J.

    2013-12-01

    The pallid sturgeon (Scaphirynchus albus) inhabits sandy-bedded rivers in the Mississippi River basin including the Missouri and Lower Yellowstone Rivers and has experienced decline generally associated with the fragmentation and alteration of these river systems. Knowledge gaps in the life history of the pallid sturgeon include lack of an understanding of conditions needed for successful reproduction and recruitment. We employed hydroacoustic tools to investigate habitats utilized by spawning pallid sturgeon in the Missouri River in Missouri, Kansas, Iowa, and Nebraska, and the Yellowstone River in Montana and North Dakota USA from 2008-2013. Reproductive pallid sturgeon were tracked to suspected spawning locations by field crews using either acoustic or radio telemetry, a custom mobile mapping application, and differential global positioning systems (DGPS). Female pallid sturgeon were recaptured soon after spawning events to validate that eggs had been released. Habitats were mapped at presumed spawning and embryo incubation sites using a multibeam echosounder system (MBES), sidescan sonar, acoustic Doppler current profiler, an acoustic camera and either a real-time kinematic global positioning system (RTK GPS) or DGPS. High-resolution DEM's and velocimetric maps were gridded from at a variety of scales from 0.10 to 5 meters for characterization and visualization at spawning and presumed embryo incubation sites. Pallid sturgeon spawning sites on the Missouri River are deep (6-8 meters) and have high current velocities (>1.5 meters per second). These sites are also characterized by high turbidity and high rates of bedload sediment transport in the form of migrating sand dunes. Spawning on the channelized Lower Missouri River occurs on or adjacent to coarse angular bank revetment or bedrock. Collecting biophysical information in these environmental conditions is challenging, and there is a need to characterize the substrate and substrate condition at a scale

  17. Application of non-lethal stable isotope analysis to assess feeding patterns of juvenile pallid sturgeon Scaphirhynchus albus: A comparison of tissue types and sample preservation methods

    Science.gov (United States)

    Andvik, R.T.; VanDeHey, J.A.; Fincel, M.J.; French, William E.; Bertrand, K.N.; Chipps, S.R.; Klumb, R.A.; Graeb, B.D.S.

    2010-01-01

    Traditional techniques for stable isotope analysis (SIA) generally require sacrificing animals to collect tissue samples; this can be problematic when studying diets of endangered species such as the pallid sturgeon Scaphirhynchus albus. Our objectives were to (i) determine if pectoral fin tissue (non-lethal) could be a substitute for muscle tissue (lethal) in SIA of juvenile pallid sturgeon, and (ii) evaluate the influence of preservation techniques on stable isotope values. In the laboratory, individual juvenile pallid sturgeon were held for up to 186 day and fed chironomids, fish, or a commercially available pellet diet. Significant, positive relationships (r2 ??? 0.8) were observed between fin and muscle tissues for both ??15N and ??13C; in all samples isotopes were enriched in fins compared to muscle tissue. Chironomid and fish based diets of juvenile pallid sturgeon were distinguishable for fast growing fish (0.3 mm day-1) using stable ??15N and ??13C isotopes. Frozen and preserved fin tissue ??15N isotopes were strongly related (r2 = 0.89) but ??13C isotopes were weakly related (r2 = 0.16). Therefore, freezing is recommended for preservation of fin clips to avoid the confounding effect of enrichment by ethanol. This study demonstrates the utility of a non-lethal technique to assess time integrated food habits of juvenile pallid sturgeon and should be applicable to other threatened or endangered species. ?? 2010 Blackwell Verlag, Berlin.

  18. Kootenai River White Sturgeon Recovery Implementation Plan and Schedule; 2005-2010, Technical Report 2004-2005.

    Energy Technology Data Exchange (ETDEWEB)

    Anders, Paul

    2007-03-01

    Kootenai River white sturgeon have been declining for at least 50 years and extinction of the wild population is now imminent (Paragamian et al. 2005). Only 630 adults were estimated to remain in 2002 from a population ten times that size just 20 years ago. Significant recruitment of young sturgeon has not been observed since the early 1970s and consistent annual recruitment has not been seen since the 1950s. The remaining wild population consists of a cohort of large, old fish that is declining by about 9% per year as fish die naturally and are not replaced. At this rate, the wild population will disappear around the year 2040. Numbers have already reached critical low levels where genetic and demographic risks are acute. The Kootenai River White Sturgeon Recovery Team was convened in 1994, provided a draft Recovery Plan in 1996 and the first complete Recovery Plan for Kootenai River white sturgeon in 1999 (USFWS 1996, 1999). The Plan outlined a four part strategy for recovery, including: (1) measures to restore natural recruitment, (2) use of conservation aquaculture to prevent extinction, (3) monitoring survival and recovery, and (4) updating and revising recovery plan criteria and objectives as new information becomes available. Sturgeon recovery efforts are occurring against a backdrop of a broader ecosystem protection and restoration program for the Kootenai River ecosystem. With abundance halving time of approximately 8 years, the Kootenai River white sturgeon population is rapidly dwindling, leaving managers little time to act. Decades of study consistently indicate that recruitment failure occurs between embryo and larval stages. This assertion is based on four key observations. First, almost no recruitment has occurred during the last 30 years. Second, thousands of naturally produced white sturgeon embryos, most viable, have been collected over the past decade, resulting from an estimated 9 to 20 spawning events each year. Third, Kootenai River white

  19. REARING OF PELED (COREGONUS PELED Gmelin IN POLYCULTURE WITH CYPRINIDS (CYPRINIDAE AND STURGEONS (ACIPENSERIDAE

    Directory of Open Access Journals (Sweden)

    G. Kurinenko

    2016-03-01

    Full Text Available Purpose. To analyze the results of rearing and provide aquaculture-biological characteristic of peled reared in polyculture with sturgeons and cyprinids based on pond technology. Methodology. The material for the studies were fry, young-of-the-year, yearlings and age-1+ peled produced from eggs exported in March 2009 from Russian Federation. Rearing of peled was carried out based on the technology developed by the All-Union Scientific and Research Institute of Pond Fish Culture for coregonids with the use of methodical recommendations on the biotechnology of industrial rearing of seed coregonids. Studies were carried out at the pond fish farm “Korop” of Lviv region. Water supply of rearing ponds was done by self-flow. The investigation of fish diet and hydrobiological studies were carried out using conventional methods. Findings. We performed a study of fish egg incubation and produced larvae with their further rearing in floating cages to the fingerling stage. Rearing of peled in polyculture allows increasing the fish productivity parameters at the first year of rearing by 1.3%, at the second year by 0.9%. Average weights of age-1 and age-1+ peled were 185.3 g and 450 g, respectively. In these rearing conditions, daily growth of the young-of-the-year was within 0.1-1.5 g, age-1+ – 1.1-3.3 g. As a positive result of rearing, we should note high weight gain during winter period that was more than 50%. We also investigated qualitative and quantitative composition of zooplankton and peled juvenile diet. Originality. The works of peled rearing based on pond technology in polyculture with sturgeons and cyprinids were carried out in the conditions of Ukraine for the first time. Practical value. The results of the performed works along with similar previous works on peled rearing in ponds will be used for the creation of methodical recommendations on rearing of peled seeds, which will be used by Ukrainian fish farms in future.

  20. The effects on growth and survival of probiotic Bacillus spp. fed to Persian sturgeon (Acipencer persicus) larvae

    OpenAIRE

    Moein Faramarzi; Hojatollah Jafaryan; Amin Farahi; M. Lashkar Boloki; Farnaz Iranshahi

    2011-01-01

    This study addressed if sturgeon larvae grew and survived better when fed a probiotic.Bacillus spp. were bioencapsulated in Daphnia magna (Straus 1820) and fed toAcipencer persicus(Borodin 1897) larvae. Bacillus bacteria (three species in a commercial preparation, Protexin Aquatic)were bioencapsulated within Daphnia at three concentrations by holding the Daphnia in suspensions of1×107, 2×107or 3×107 bacteria per milliliter for 10 hours. The sturgeon larvae were fed one of the three probiotic ...

  1. Chinese Dream

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The general managers of South Korean auto giants Hyundai and Kia have high hopes for the growing Chinese auto market. Both companies went through a painstaking period as the financial crisis first roared across the globe. Jin Shan-fa, General Manager of Hyundai Motor Group

  2. Decision 99-18: Range Petroleum Corporation application for a well licence, Sturgeon Lake Area

    International Nuclear Information System (INIS)

    Range Petroleum Corporation (Range) applied to the Alberta Energy and Utilities Board (EUB) pursuant to Section 2.020 of the Oil and Gas Conservation Regulations for a well licence to drill a sour oil well from a surface location in Legal Subdivision 4, Section 34, West of the fifth Meridian, directionally to a bottom-hole location under Sturgeon Lake. The purpose of the proposed well would be to obtain sour oil production from the Leduc Formation. The well would be a Level 1 well because it would have a potential maximum hydrogen sulphide release rate of 0.0412 cubic m/s. The EUB received objections to the application from landowners, cattle ranchers, farmers, residents, and cottage owners in the area of the proposed well. The application and intervention were considered at a public hearing on 26 January 1999 in Grande Prairie, Alberta, and the Board viewed the proposed surface location, the previous Lds 3-2 surface location, and the surrounding area prior to the hearing. Having carefully considered the evidence, the Board believed that it would be possible to drill the proposed well safely and with minimal risk, subject to attached conditions. But in light of the unique setting of the area, including the residences and the road and lake configurations, and the difficulty that would exist preparing an effective environmental review process (ERP), the Board deferred its decision on the well licence application until an approved ERP is in place

  3. Růstové vlastnosti a problematika chovu jesetera sibiřského (Acipenser baerii) v podmínkách České republiky

    Czech Academy of Sciences Publication Activity Database

    Prokeš, Miroslav; Baruš, Vlastimil; Peňáz, Milan; Baránek, V.

    2003-01-01

    Roč. 39, 1-2 (2003), s. 99-103. ISSN 0007-389X. [Nové technologie a nové druhy v akvakultuře.. Vodňany, 24.04.2003-25.04.2003] R&D Projects: GA AV ČR IAA6093104; GA AV ČR KSK6005114 Institutional research plan: CEZ:AV0Z6093917 Keywords : Acipenser baerii * growth rate Subject RIV: GL - Fishing

  4. Effects of Mitigation Measures on Productivity of the White Sturgeon Populations in the Columbia River Downstream from McNary Dam, and Status and Habitat Requirements of White Sturgeon Populations in the Columbia and Snake Rivers Upstream from McNary Dam, 1992-1993 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Beamesdorfer, Raymond C.; Nigro, Anthony A. (Oregon Department of Fish and Wildlife, Portland, OR)

    1993-12-01

    We report on our progress from April 1992-March 1993 in research on white sturgeon in the lower Columbia River. The study began in July 1986 and progress through 1992 was summarized in a comprehensive report in 2 volumes (Beamesderfer and Nigro 1993a, 1993b). This report details activities during the first year of Phase II of this sturgeon research. In Phase I, we assessed the status and habitat requirements of the white sturgeon populations in the Columbia River downstream from McNary Dam. Phase II will examine the effects on white sturgeon productivity of mitigative measures recommended in Phase I. The status and habitat requirements of white sturgeon populations upstream from McNary Dam will also be examined in Phase II. The study is a cooperative effort by the Oregon Department of Fish and Wildlife, Washington Department of Fisheries, U.S. Fish and Wildlife Service, and National Marine Fisheries Service. Work during the past year has focused on: (1) analysis of results of limited sampling conducted in 1992, (2) submission of Phase I results to the peer-review literature to ensure widespread dissemination, clarity of presentation, and credibility of findings, and (3) preparations for additional field work in 1993. In report sections A to D, each agency reports 1992 results if applicable and the current status of manuscripts. Results of field work conducted in 1993 will be reported in the 1994 annual report.

  5. Status and habitat requirements of white sturgeon populations in the Columbia River downstream from McNary Dam, annual progress report, July 1986 - March 1987.; ANNUAL

    International Nuclear Information System (INIS)

    Measure 804(e)(8) of the Northwest Power Planning Council (NPPC) Fish and Wildlife Program states that Bonneville Power Administration (BPA) ''shall fund research to determine the impacts of development and operation of the hydroelectric power system on sturgeon in the Columbia River Basin...'' In June 1985, BPA sponsored a workshop to define and list in priority order research needs in the basin (Fickeisen 1985a). In December 1985, BPA submitted a research program implementation plan (Fickeisen 1985b) to the NPPC. The purpose of the plan is to provide guidance for conducting research necessary to address four objectives identified by regional fishery interests for protecting, mitigating and enhancing white sturgeon populations in the Columbia River basin. The plan's objectives are: (1) Assess the current status of Columbia River basin white sturgeon stocks. (2) Provide the basis to evaluate the need for protection, mitigation and enhancement of white sturgeon in the Columbia River system. (3) Provide information that can be used to evaluate potential methods of protection, mitigation and enhancement of existing stocks. (4) Provide tools to assess the effectiveness of protection, mitigation and enhancement efforts

  6. Status and Habitat Requirements of White Sturgeon Populations in the Columbia River Downstream from McNary Dam, 1986-1987 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    McCabe, Jr., George T. (National Marine Fisheries Service, Environmental and Technical Services Division, Portland, OR); Beckman, Lance G. (US Fish and Wildlife Service, Portland, OR); Kreitman, Gayle (Washington Department of Fisheries, Olympia, WA)

    1987-06-01

    Measure 804(e)(8) of the Northwest Power Planning Council (NPPC) Fish and Wildlife Program states that Bonneville Power Administration (BPA) ''shall fund research to determine the impacts of development and operation of the hydroelectric power system on sturgeon in the Columbia River Basin...'' In June 1985, BPA sponsored a workshop to define and list in priority order research needs in the basin (Fickeisen 1985a). In December 1985, BPA submitted a research program implementation plan (Fickeisen 1985b) to the NPPC. The purpose of the plan is to provide guidance for conducting research necessary to address four objectives identified by regional fishery interests for protecting, mitigating and enhancing white sturgeon populations in the Columbia River basin. The plan's objectives are: (1) Assess the current status of Columbia River basin white sturgeon stocks. (2) Provide the basis to evaluate the need for protection, mitigation and enhancement of white sturgeon in the Columbia River system. (3) Provide information that can be used to evaluate potential methods of protection, mitigation and enhancement of existing stocks. (4) Provide tools to assess the effectiveness of protection, mitigation and enhancement efforts.

  7. An experimental test and models of drift and dispersal processes of pallid sturgeon (Scaphirhynchus albus) free embryos in the Missouri River

    Science.gov (United States)

    Braaten, P.J.; Fuller, D.B.; Lott, R.D.; Ruggles, M.P.; Brandt, T.F.; Legare, R.G.; Holm, R.J.

    2012-01-01

    Free embryos of wild pallid sturgeon Scaphirhynchus albus were released in the Missouri River and captured at downstream sites through a 180-km reach of the river to examine ontogenetic drift and dispersal processes. Free embryos drifted primarily in the fastest portion of the river channel, and initial drift velocities for all age groups (mean = 0.66–0.70 m s−1) were only slightly slower than mean water column velocity (0.72 m s−1). During the multi-day long-distance drift period, drift velocities of all age groups declined an average of 9.7% day−1. Younger free embryos remained in the drift upon termination of the study; whereas, older age groups transitioned from drifting to settling during the study. Models based on growth of free embryos, drift behavior, size-related variations in drift rates, and channel hydraulic characteristics were developed to estimate cumulative distance drifted during ontogenetic development through a range of simulated water temperatures and velocity conditions. Those models indicated that the average free embryo would be expected to drift several hundred km during ontogenetic development. Empirical data and model results highlight the long-duration, long-distance drift and dispersal processes for pallid sturgeon early life stages. In addition, results provide a likely mechanism for lack of pallid sturgeon recruitment in fragmented river reaches where dams and reservoirs reduce the length of free-flowing river available for pallid sturgeon free embryos during ontogenetic development.

  8. Chinese restaurant syndrome

    Science.gov (United States)

    Chinese restaurant syndrome is a set of symptoms that some people have after eating Chinese food. A food additive ... Chinese restaurant syndrome is most often diagnosed based on the symptoms. The health care provider may ask the following ...

  9. CHINESE JOURNAL OF CHEMISTRY

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@Chinese Journal of Chemistry is an international journal published in English by the Chinese Chemical Society with its editorial office hosted by Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences.

  10. Chinese Culture and Leadership.

    Science.gov (United States)

    Wong, Kam-Cheung

    2001-01-01

    Describes essential characteristics of Chinese philosophical tradition; Discusses Western perspectives on value leadership in education, particularly moral leadership. Discuses moral leadership from a Chinese philosophical perspective, especially Confucianism. Draws implications for using Chinese cultural and philosophical traditions to develop…

  11. The Chinese Banking System

    OpenAIRE

    Grant Turner; Nicholas Tan; Dena Sadeghian

    2012-01-01

    The Chinese banking system is critical to the functioning of the Chinese economy, being the main conduit through which savings are allocated to investment opportunities. Banking activity in China has grown rapidly over the past decade in association with the expansion of the Chinese economy, and the Chinese banking system now includes some of the world’s largest banks. Chinese banks have become more commercially orientated over this period, although the Chinese Government retains considerable...

  12. White Sturgeon Management Plan in the Snake River between Lower Granite and Hells Canyon Dams; Nez Perce Tribe, 1997-2005 Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Nez Perce Tribe Resources Management Staff, (Nez Perce Tribe, Department of Fisheries Resource Management, Lapwai, ID)

    2005-09-01

    White sturgeon in the Hells Canyon reach (HCR) of the Snake River are of cultural importance to the Nez Perce Tribe. However, subsistence and ceremonial fishing opportunities have been severely limited as a result of low numbers of white sturgeon in the HCR. Hydrosystem development in the Columbia River Basin has depressed numbers and productivity of white sturgeon in the HCR by isolating fish in impounded reaches of the basin, restricting access to optimal rearing habitats, reducing the anadromous forage base, and modifying early life-history habitats. Consequently, a proactive management plan is needed to mitigate for the loss of white sturgeon production in the HCR, and to identify and implement feasible measures that will restore and rebuild the white sturgeon population to a level that sustains viability and can support an annual harvest. This comprehensive and adaptive management plan describes the goals, objectives, strategies, actions, and expected evaluative timeframes for restoring the white sturgeon population in the HCR. The goal of this plan, which is to maintain a viable, persistent population that can support a sustainable fishery, is supported by the following objectives: (1) a natural, stable age structure comprising both juveniles and a broad spectrum of spawning age-classes; (2) stable or increasing numbers of both juveniles and adults; (3) consistent levels of average recruitment to ensure future contribution to reproductive potential; (4) stable genetic diversity comparable to current levels; (5) a minimum level of abundance of 2,500 adults to minimize extinction risk; and (6) provision of an annual sustainable harvest of 5 kg/ha. To achieve management objectives, potential mitigative actions were developed by a Biological Risk Assessment Team (BRAT). Identified strategies and actions included enhancing growth and survival rates by restoring anadromous fish runs and increasing passage opportunities for white sturgeon, reducing mortality rates

  13. Effects of Beta-Naphthoflavone and 3-Methylcholantheren on Biochemical Markers in Sturgeon Fish, Huso huso

    Directory of Open Access Journals (Sweden)

    Katayoon Karimzadeh

    2016-04-01

    Full Text Available Background: Polycyclic aromatic hydrocarbons (PAHs are a predominant group of contaminants that have been shown to accumulate and persist in marine organisms. We evaluated the dose-dependent induction of the cytochrome P4501A (CYP1A system in the Sturgeon fish, Huso huso after after exposure to PAHs, beta-naphthoflavone (BNF and 3- methylcholantheren. Methods: The fish were treated by i.p. injections of beta–naphthoflavone and 3- methylcholantheren dissolved in corn oil at three doses 35, 70 and 105 mg/kg wet-body weight respectively for 72 h every day. Similarly, the control fish received injections of only corn oil. Microsomal fraction was prepared and ethoxy resorufin-O-deethylase activity (EROD was measured in the fish liver. Cytochrome P4501A1 (CYP1A1 content was estimated by ELISA based on monoclonal anti-cod P4501A1. Results: Beta-naphthoflovone and 3-methylcholantheren treated-fish developed 15-32 fold increase in Ethoxyresorufin-O-deethylase activity in liver microsomes. The results of ELISA showed higher levels of cytochrome P4501A content in treated fish as compared to controls. Conclusion: A parallel increase of the CYP1A immunochemically assay and an increase in EROD activity could be recorded for beta –naphthoflavone and 3- methylcholantheren in injected fish. Dose-response relationships on the induction of EROD and CYP1A immunodetectable protein were recorded. Therefore, EROD activity and CYP1A content can be used as a biomarker of polycyclic aromatic hydrocarbons.

  14. White Sturgeon Mitigation and Restoration in the Columbia and Snake Rivers Upstream from Bonneville Dam, 1999-2000 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ward, David L. (Oregon Department of Fish and Wildlife, Portland, OR)

    2001-04-01

    We report on our progress from April 1999 through March 2000 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam. The study is a cooperative effort by the Oregon Department of Fish and Wildlife (ODFW; Report A), Washington Department of Fish and Wildlife (WDFW; Report B), U.S. Geological Survey Biological Resources Division (USGS; Report C), Columbia River Inter-Tribal Fish Commission (CRITFC; Report D), and the U.S. Fish and Wildlife Service (USFWS; Report E). This is a multi-year study with many objectives requiring more than one year to complete. Therefore, findings from a given year may be part of more significant findings yet to be reported. Highlights of results of our work from April 1999 through March 2000 are given.

  15. White Sturgeon Mitigation and Restoration in the Columbia and Snake Rivers Upstream from Bonneville Dam; 1998-1999 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ward, David L.

    2000-12-01

    The authors report on their progress from April 1998 through March 1999 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam. The study is a cooperative effort by the Oregon Department of Fish and Wildlife (ODFW; Report A), Washington Department of Fish and Wildlife (WDFW; Report B), U.S. Geological Survey Biological Resources Division (USGS; Report C), U.S. Fish and Wildlife Service (USFWS; Report D), Columbia River Inter-Tribal Fish Commission (CRITFC; Report E), and the University of Idaho (UI; Report F). This is a multi-year study with many objectives requiring more than one year to complete. Therefore, findings from a given year may be part of more significant findings yet to be reported. Highlights of results of our work from April 1998 through March 1999 are given.

  16. The effects on growth and survival of probiotic Bacillus spp. fed to Persian sturgeon (Acipencer persicus larvae

    Directory of Open Access Journals (Sweden)

    Moein Faramarzi

    2011-01-01

    Full Text Available This study addressed if sturgeon larvae grew and survived better when fed a probiotic.Bacillus spp. were bioencapsulated in Daphnia magna (Straus 1820 and fed toAcipencer persicus(Borodin 1897 larvae. Bacillus bacteria (three species in a commercial preparation, Protexin Aquaticwere bioencapsulated within Daphnia at three concentrations by holding the Daphnia in suspensions of1×107, 2×107or 3×107 bacteria per milliliter for 10 hours. The sturgeon larvae were fed one of the three probiotic treatments at a level of 30 percent body weight 5 times a day. The growth and survival oflarvae fed the protbiotic enriched Daphnia were compared to those larvae fed a control treatment ofunbioencapsulated Daphnia magna. The results showed that larvae fed the probioticBacillus spp. hadincreased final body weight and specific growth rate in comparison to control treatment. The probioticbacillus also had significant positive effects on daily growth ratio, daily growth coefficient, averageweight gain and survival in comparison to those fed the control treatment. The food conversation ratiowas the one factor that decreased significantly in comparison with the control treatment. The amount ofprobiotic used did not improve performance, so larviculture can be increased merely by the addition of oflow level of probiotics.

  17. White Sturgeon Mitigation and Restoration in the Columbia and Snake Rivers Upstream from Bonneville Dam; 2000-2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Kern, J. Chris; Ward, David L.; Farr, Ruth A. (Oregon Department of Fish and Wildlife)

    2002-02-01

    We report on our progress from April 2000 through March 2001 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam. The study is a cooperative effort by the Oregon Department of Fish and Wildlife (ODFW; Report A), Washington Department of Fish and Wildlife (WDFW; Report B), U.S. Geological Survey Biological Resources Division (USGS; Report C), Columbia River Inter-Tribal Fish Commission (CRITFC; Report D), the U.S. Fish and Wildlife Service (USFWS; Report E), and Oregon State University (OSU; Report F). This is a multi-year study with many objectives requiring more than one year to complete; therefore, findings from a given year may be part of more significant findings yet to be reported. Highlights of results of our work from April 2000 through March 2001 are listed.

  18. Characterization of environmental cues for initiation of reproductive cycling and spawning in shovelnose sturgeon Scaphirhynchus platorynchus in the Lower Missouri River, USA

    Science.gov (United States)

    Papoulias, D.M.; DeLonay, A.J.; Annis, M.L.; Wildhaber, M.L.; Tillitt, D.E.

    2011-01-01

    We presume that the shovelnose sturgeon (Scaphirhynchus platorynchus) has evolved to spawn in the springtime when environmental conditions are at some optimum, but this state has not yet been defined. In this study physiological readiness to spawn in shovelnose sturgeon was examined to define more closely when spawning could occur and thus identify and evaluate prevailing environmental conditions that could cue spawning during that period. Reproductive assessments of Lower Missouri River shovelnose during 4 years (2005-2008) and at two locations (Gavins Point Dam, South Dakota and Boonville, Missouri) were used to identify shovelnose sturgeon spawning periods. Initiation of the spawning period, as defined by the presence of reproductively ready fish, was a highly predictable yearly event and extended over several weeks at each reach. The spawning period occurred earlier in the lower reach than in the upper reach and environmental conditions during the periods varied between locations and among years. Shovelnose sturgeon collected during the presumed spawning periods were at varying degrees of readiness to spawn as indicated by oocyte polarization index and blood reproductive hormones. Evaluation of the influence of environmental factors on readiness to spawn using stepwise multiple regression analysis indicated photoperiod followed by temperature were the best candidate variables overall to explain the trend. However, within geographically distinct populations gravid females are not all reproductively synchronized. Assuming that this apparent asynchrony in readiness is normal and not an artifact of the disturbed Missouri River system, we infer that individual sturgeon can persist in a reproductively ready state until conditions appropriate for spawning occur. Taken together, our results lead us to hypothesize that gravid females early in the reproductive cycle (post-vitellogenesis) respond to day length, a reliable annual cue, become increasingly more ready to

  19. Sturgeon hatching enzyme and the mechanism of egg envelope digestion: Insight into changes in the mechanism of egg envelope digestion during the evolution of ray-finned fish.

    Science.gov (United States)

    Nagasawa, Tatsuki; Kawaguchi, Mari; Sano, Kaori; Yasumasu, Shigeki

    2015-12-01

    We investigated the evolution of the hatching enzyme gene using bester sturgeon (hybrid of Acipencer ruthenus and Huso huso), a basal member of ray-finned fishes. We purified the bester hatching enzyme from hatching liquid, yielding a single band on SDS-PAGE, then isolated its cDNA from embryos by PCR. The sturgeon hatching enzyme consists of an astacin family protease domain and a CUB domain. The CUB domains are present in frog and bird hatching enzymes, but not in teleostei, suggesting that the domain structure of sturgeon hatching enzyme is the tetrapod type. The purified hatching enzyme swelled the egg envelope, and selectively cleaved one of five egg envelope proteins, ZPAX. Xenopus hatching enzyme preferentially digests ZPAX, thus, the egg envelope digestion process is conserved between amphibians and basal ray-finned fish. Teleostei hatching enzymes cleave the repeat sequences at the N-terminal region of ZPB and ZPC, suggesting that the targets of the teleostei hatching enzymes differ from those of amphibians and sturgeons. Such repeat sequences were not found in the N-terminal region of ZPB and ZPC of amphibians and sturgeons. Our results suggest that the change in substrates of the hatching enzymes was accompanied by the mutation of the amino acid sequence of N-terminal regions of ZPB and ZPC. We conclude that the changes in the mechanism of egg envelope digestion, including the change in the domain structure of the hatching enzymes and the switch in substrate, occurred during the evolution of teleostei, likely triggered by the teleost-specific third whole genome duplication. J. Exp. Zool. (Mol. Dev. Evol.) 324B: 720-732, 2015. © 2015 Wiley Periodicals, Inc. PMID:26514945

  20. Chinese Calendar and Chinese Telegraphic Code.

    Science.gov (United States)

    Defense Language Inst., Monterey, CA.

    This manual contains: (1) Chinese calendars for the hundred years from 1881 to 1980; and (2) the Chinese telegraphic code. Each page in Part One presents the calendar for each year in both Chinese and English. There are 97 charts in Part Two representing the telegraphic code. (AMH)

  1. COMPARATIVE STUDY ON THE HORMONAL STIMULATION STERLET (ACIPENSER RUTHENUS, LINNAEUS, 1758 USING CARP HYPOPHYSIS AND ARTIFICIAL HORMONE TYPE NERESTIN 5-5A

    Directory of Open Access Journals (Sweden)

    R. C. DIMA

    2013-07-01

    Full Text Available Starting from the particular sturgeon reproductive biology, propagation of technology through artificial comprises a number of phases required including: selection, parking broodstocks cell maturation and sexual stimulation in order fertilized their conditions provided. To stimulate the process of sexual maturation of the sexual elements for sterlet could be hypophysis and also analogue gonadothropic hormone type Nerestin 5. Literature attesting to the use of sterlet dry hypophysis treated with acetone (Maria Caloianu-Iordăchel, 1973 and employing successful hormone analogue found as LHRHa (N.Patrichi and collaborators, 1989- unpublished data. Advantages and disadvantages of using hypophysis or gonadotropic hormone analogue fdhgghNerestin 5 are very important and decisive for modern aquaculture the century XXI century.

  2. Management of Chinese restaurant

    OpenAIRE

    Cui , Longbo

    2009-01-01

    With Chinese economy developing rapidly, the Chinese restaurant is under the spotlight, but the management of Chinese restaurant is weak at the moment, especially on the service management, which is an important part of service management in the Chinese restaurant. On the other hand, the managers of Chinese restaurant should pay more attention on the service management for instance brand, service innovation. Service management is core and essential concept for every service company recently, ...

  3. Visual Basic, Excel-based fish population modeling tool—The pallid sturgeon example

    Science.gov (United States)

    Moran, Edward H.; Wildhaber, Mark L.; Green, Nicholas S.; Albers, Janice L.

    2016-01-01

    The model presented in this report is a spreadsheet-based model using Visual Basic for Applications within Microsoft Excel (http://dx.doi.org/10.5066/F7057D0Z) prepared in cooperation with the U.S. Army Corps of Engineers and U.S. Fish and Wildlife Service. It uses the same model structure and, initially, parameters as used by Wildhaber and others (2015) for pallid sturgeon. The difference between the model structure used for this report and that used by Wildhaber and others (2015) is that variance is not partitioned. For the model of this report, all variance is applied at the iteration and time-step levels of the model. Wildhaber and others (2015) partition variance into parameter variance (uncertainty about the value of a parameter itself) applied at the iteration level and temporal variance (uncertainty caused by random environmental fluctuations with time) applied at the time-step level. They included implicit individual variance (uncertainty caused by differences between individuals) within the time-step level.The interface developed for the model of this report is designed to allow the user the flexibility to change population model structure and parameter values and uncertainty separately for every component of the model. This flexibility makes the modeling tool potentially applicable to any fish species; however, the flexibility inherent in this modeling tool makes it possible for the user to obtain spurious outputs. The value and reliability of the model outputs are only as good as the model inputs. Using this modeling tool with improper or inaccurate parameter values, or for species for which the structure of the model is inappropriate, could lead to untenable management decisions. By facilitating fish population modeling, this modeling tool allows the user to evaluate a range of management options and implications. The goal of this modeling tool is to be a user-friendly modeling tool for developing fish population models useful to natural resource

  4. Effects of Mitigative Measures on Productivity of White Sturgeon Populations in the Columbia River Downstream from McNary Dam; Determine Status and Habitat Requirements of White Sturgeon Populations in the Columbia and Snake Rivers Upstream from McNary Dam, 1995-1996 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Rien, Thomas A.; Beiningen, Kirk T. (Oregon Department of Fish and Wildlife, Portland, OR)

    1997-07-01

    This project began in July 1986 and is a cooperative effort of federal, state, and tribal fisheries entities to determine (1) the status and habitat requirements, and (2) effects of mitigative measures on productivity of white sturgeon populations in the lower Colombia and Snake rivers.

  5. Effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and status and habitat requirements of white sturgeon populations in the Columbia and Snake Rivers upstream from McNary Dam

    International Nuclear Information System (INIS)

    This report details activities during the first year of Phase II of this sturgeon research. In Phase I, the authors assessed the status and habitat requirements of the white sturgeon populations in the Columbia River downstream from McNary Dam. Phase II will examine the effects on white sturgeon productivity of mitigative measures recommended in Phase I. The status and habitat requirements of white sturgeon populations upstream from McNary Dam will also be examined in Phase II. The study is a cooperative effort by the Oregon Department of Fish and Wildlife, Washington Department of Fisheries, US Fish and Wildlife Service, and National Marine Fisheries Service. Work during the past year has focused on: (1) analysis of results of limited sampling conducted in 1992, (2) submission of Phase I results to the peer-review literature to ensure widespread dissemination, clarity of presentation, and credibility of findings, and (3) preparation for additional field work in 1993. In report sections A to D, each agency reports 1992 results if applicable and the current status of manuscripts. Results of field work conducted in 1993 will be reported in the 1994 annual report

  6. STUDIES REGARDING THE PRESENCE OF THE PATHOGENS BACTERIA INTO A RECIRCULATING SYSTEM OF BELUGA STURGEON INTENSIVE REARING

    Directory of Open Access Journals (Sweden)

    ANGELICA DOCAN

    2013-12-01

    Full Text Available Recirculating aquaculture offers good potential for successful fish farming since is often independent of environmental conditions. Maintaining healthy fish in a recirculating system involves establishing adequate dissolved oxygen levels, removal of solid wastes, and sufficient ammonia nitrification to assure optimal rearing conditions. Neglecting these, the fish immune system will depress, the facultative pathogen germs will be able to provoke important disease outbreaks into cultured biomass, as was happened in our recirculating pilot system. In this study are presented the pathological aspects registered to the beluga sturgeon of 1 year, reared into our recirculating pilot system, pathological aspects generated by a haemorrhagic bacterial septicaemia which was manifested in the conditions of low concentrations of DO. The disease was diagnosed to the affected fish through anatomopathological and clinical exam, haematological exam and microbiological exam.

  7. Status and habitat requirements of the white sturgeon populations in the Columbia River downstream from McNary Dam

    International Nuclear Information System (INIS)

    We report on our progress from April 1990 through March 1991 on determining the status and habitat requirements of white sturgeon populations in the Columbia River downstream from McNary Dam. The study is a cooperative effort by the Oregon Department of Fish and Wildlife (ODFW), Washington Department of Fisheries (WDF), US Fish and Wildlife Service (FWS) and National Marine Fisheries Service (NMFS). Study objectives addressed by each agency are to describe the life history and population dynamics of subadults and adults between Bonneville and McNary dams and evaluate the need and identify potential methods for protecting, mitigating and enhancing populations downstream from NcNary Dam; to describe the white sturgeon recreational fishery between Bonneville and McNary dams, describe reproductive and early life history characteristics downstream from Bonneville Dam and describe life history and population dynamics of subadults and adults downstream from Bonneville Dam; to describe reproduction and early life history characteristics, define habitat requirements for spawning and rearing and quantify extent of habitat available between Bonneville and McNary dams; and to describe reproduction and early life history characteristics, define habitat requirements for spawning and rearing and quantify extent of habitat available downstream from Bonneville Dam. Our approach is to work concurrently downstream and upstream from Bonneville Dam. Upstream from Bonneville Dam we began work in the Dalles Reservoir in 1987 and expanded efforts to Bonneville Reservoir in 1988 and John Day Reservoir in 1989. Highlights of results of this work in the Dalles, Bonneville and John Day reservoirs are included in the four pages included in this report

  8. Effects of dietary Spirulina platensis on growth performance, humoral and mucosal immune responses and disease resistance in juvenile great sturgeon (Huso huso Linnaeus, 1754).

    Science.gov (United States)

    Adel, Milad; Yeganeh, Sakineh; Dadar, Maryam; Sakai, Masahiro; Dawood, Mahmoud A O

    2016-09-01

    Dietary supplementation of Spirulina platensis at different levels (0% control, 2.5%, 5% and 10%) was evaluated to find out the effects on growth performance, digestive enzyme activities, humoral and skin innate immune responses and disease resistance in the great sturgeon (Huso huso). After 8 weeks of experimental trial, growth parameters, intestinal lactic acid bacteria count, protease and lipase activities were significantly high in 10% S. platensis fed group (P bacteria increased mortality, but it was alleviated by a diet supplemented with S. platensis. The present results demonstrate that this dietary supplementation with S. platensis (mainly at 10% level) could be useful for maintaining the overall health status of great sturgeon. PMID:27506276

  9. White Sturgeon Mitigation and Restoration in the Columbia and Snake Rivers Upstream from Bonneville Dam; Annual Progress Report, April 2007 - March 2008.

    Energy Technology Data Exchange (ETDEWEB)

    Mallette, Christine [Oregon Department of Fish and Wildlife

    2009-07-28

    We report on our progress from April 2007 through March 2008 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam. The study is a cooperative effort by the Oregon Department of Fish and Wildlife (ODFW; Report A), Washington Department of Fish and Wildlife (WDFW; Report B), Columbia River Inter-Tribal Fish Commission (CRITFC; Report C), and Montana State University (MSU; Report D). This is a multi-year study with many objectives requiring more than one year to complete; therefore, findings from a given year may be part of more significant findings yet to be reported.

  10. Růst jesetera malého (Acipenser ruthenus) v podmínkách rybníkářství Pohořelice

    Czech Academy of Sciences Publication Activity Database

    Baránek, V.; Prokeš, Miroslav; Baruš, Vlastimil; Peňáz, Milan; Mareš, J.; Spurný, P.; Němec, R.

    Brno : Mendelova zemědělská a lesnická univerzita, 2004 - (Spurný, P.), s. 119-127 ISBN 80-7157-810-X. [55 let výuky rybářské specializace na MZLU v Brně. Brno (CZ), 30.11.2004-01.12.2004] R&D Projects: GA MZe QF3028; GA AV ČR KSK6005114 Institutional research plan: CEZ:AV0Z6093917 Keywords : Acipenser ruthenus Subject RIV: GL - Fishing

  11. Contributions to understanding the fall migration of beluga sturgeon (Huso huso) on the Lower Danube River, Romania

    OpenAIRE

    Alin M. Bâdiliţă; György Deák; Carmen G. Nicolae; Ştefan Diaconescu

    2013-01-01

    Within this article the authors present the processing of an informational volume, unique on bothnational and international plan, referring to sturgeons’ migration on Danube. In order to present the results ofthe monitoring activity, carried out by a researching mix team of the above mentioned institutes, we chose themarine sturgeon beluga (Huso huso). The objective is to offer exclusive results regarding the migration of thissturgeon species in order to elaborate some protection and conserva...

  12. A fully-stochasticized, age-structured population model for population viability analysis of fish: Lower Missouri River endangered pallid sturgeon example

    Science.gov (United States)

    Wildhaber, Mark L.; Albers, Janice; Green, Nicholas; Moran, Edward H.

    2015-01-01

    We develop a fully-stochasticized, age-structured population model suitable for population viability analysis (PVA) of fish and demonstrate its use with the endangered pallid sturgeon (Scaphirhynchus albus) of the Lower Missouri River as an example. The model incorporates three levels of variance: parameter variance (uncertainty about the value of a parameter itself) applied at the iteration level, temporal variance (uncertainty caused by random environmental fluctuations over time) applied at the time-step level, and implicit individual variance (uncertainty caused by differences between individuals) applied within the time-step level. We found that population dynamics were most sensitive to survival rates, particularly age-2+ survival, and to fecundity-at-length. The inclusion of variance (unpartitioned or partitioned), stocking, or both generally decreased the influence of individual parameters on population growth rate. The partitioning of variance into parameter and temporal components had a strong influence on the importance of individual parameters, uncertainty of model predictions, and quasiextinction risk (i.e., pallid sturgeon population size falling below 50 age-1+ individuals). Our findings show that appropriately applying variance in PVA is important when evaluating the relative importance of parameters, and reinforce the need for better and more precise estimates of crucial life-history parameters for pallid sturgeon.

  13. Happy (Chinese) New Year!

    Science.gov (United States)

    Johnson, Georgia G.

    1979-01-01

    Suggestions are made for a classroom celebration of Chinese New Year, including discussion of the Chinese calendar and customs, a short list of appropriate children's stories, and food ideas, including a recipe for fortune cookies. (SJL)

  14. Chinese restaurant syndrome

    Directory of Open Access Journals (Sweden)

    Balachandran C

    1991-01-01

    Full Text Available A 24-year-old Chinese student with history of recurrent attacks of flushing with burning and dryness of face of 4 years duration showed exacerbation of the symptoms after oral provocation with 1 mg of Chinese salt. Patient was treated with 50 mg pyridoxine daily and restriction of the Chinese salt in diet with moderate improvement.

  15. Chinese varkens in Nederland

    OpenAIRE

    Zhang, W.L.; Huiskes, J.H.

    1992-01-01

    In China zijn in totaal 300 miljoen varkens van meer dan 100 rassen. Ze worden voor verschillende soorten productie gebruikt. Sommige Chinese rassen zijn in Frankrijk, Nederland en U.S.A. geomporteerd. De vraag is of Chinese varkens nuttig kunnen zijnvoor de varkensproductie in Nederland en zo ja welke Chinese varkens

  16. [On the Population Genetic Portrait of Kaluga, Acipenser dauricus Georgi, 1775 Analysis of Sequence Variation in the Mitochondrial DNA Control Region].

    Science.gov (United States)

    Shedko, S V; Miroshnichenko, I L; Nemkova, G A; Shedko, M B

    2015-09-01

    The variability of the mtDNA D-loop was examined in kaluga endemic to the Amur River, which is classified as critically endangered by the IUCN Red List of Threatened species. Sequencing of the D-loop fragment (819 bp) in 122 kaluga specimens collected in Lower Amur revealed 27 unique genotypes. The sample was characterized by a relatively low level of haplotypic (0.927) and nucleotide (0.0044) diversity. No considerable deviations from the neutral mutation model of DNA polymorphism were observed. Overall, the mismatch distribution patterns and the results of testing of simple demographic models (sudden demographic expansion and exponential population growth) pointed to a past increase in the number of kaluga sturgeons. According to the Bayesian skyline, the kaluga population doubled over the last two to three thousand years. The number of mature females in the modern kaluga population and the assessment of their long-term effective population size (Nef) are roughly at the same level (about three thousand individuals), which confirms the validity of assigning kaluga to the category of species on the brink of extinction. PMID:26606799

  17. Chinese Food in America

    OpenAIRE

    Jou, Diana T.

    2011-01-01

    How did Chinese food get to look like this? With more than 41,000 Chinese restaurants in America - 3 times the number of McDonald’s restaurants - Chinese food is one of the most accepted and misunderstood cuisines in the United States. From large cities to small towns, locals can always count on an order of orange chicken in a takeout box, with a few fortune cookies thrown in the bag. But what Americans view as Chinese food is far from a traditional Chinese meal, wh...

  18. Danish-accented Chinese

    DEFF Research Database (Denmark)

    Wang, Lei; Sloos, Marjoleine 莱娜; Zhang, Chun

    In search for a linguistic basis for the education of Chinese as a foreign language CFL in Denmark, we set up a new line of investigation into CFL. This research focuses on the phonetics and phonology of Mandarin Chinese as compared to Danish. Considering the sound systems of both languages, we...... note some differences and similarities. The most remarkable differences are: -Chinese has rhotic sounds (pinyin ch, zh, sh, r) but Danish does not -Chinese has affricates (c z ch zh tɕ j) but Danish does not What Danish shares with Chinese is the contrast between aspirated and plain consonants: pa...

  19. Research and Development of Nutritional and Healthy Function of Sturgeon and its Deep Processing Products%鲟鱼营养保健功能及其深加工产品的开发研究

    Institute of Scientific and Technical Information of China (English)

    王蕊

    2014-01-01

    为提高鲟鱼的养殖效益,探索鲟鱼发展产业链,使更多人享用肉味鲜美、营养保健的鲟鱼产品,通过查阅文献资料,对制约鲟鱼产业瓶颈的主要因素进行了研究。结果表明,由于养殖规模的快速扩张、深加工技术研究相对滞后、农户养殖效益下降,严重制约了该产业的可持续发展。解决鲟鱼蛋白低温抗冻技术,开发鲟鱼速冻调理食品、特色风味休闲食品和针对不同消费人群的功能性食品是鲟鱼产业未来的主要发展方向。%To improve the benefit of the sturgeon breeding,then explore the d industrial chain of evelopment,the sturgeon products,which are delicious,nutrition,care,are enjoyed by more people,the main factors restraining the sturgeon industry was learned through consulting literature material.The results show that because of the rapid expansion of scale of breeding and the relative lag of deep processing technology ,so that the benefit of farmers breeding drop,severely restricted the sustainable development of this industry.So solving the antifreeze technology of sturgeon protein in low temperature,then the sturgeon frozen prepared food,unique flavor snack food and functional food for different consumers will be developed,these are the main development direction of sturgeon industry in the future.

  20. Effective properties of a sturgeon-based bioactive compound on stress-induced hippocampal degeneration and on in vitro neurogenesis.

    Science.gov (United States)

    Marotta, F; Chui, D H; Yadav, H; Lorenzetti, A; Celep, G; Jain, S; Bomba, A; Polimeni, A; Zhong, K; Allegri, F

    2012-01-01

    The aim of this study is to test the activity of a marine bioactive compound containing high-purity caviar-derived DNA, collagen elastin and protein extracts from sturgeon (LD-1227, Caviarlieri, Laboratoires Dom, Switzerland) to exert neuroprotective properties in an experimental setting while also being potential triggers of neurogenesis in a separate in vitro study. Supplementation with high-DHA mixture of LD-1227 was applied for 30 days to stress model rats. Both supplementations significantly mitigated the histological brain damage when analyzing hippocampal subregions and corticosterone level. However, LD-1227 was most significantly efficient in preventing SOD, Catalase and ascorbic acid decrease in brain tissue. Both supplementations stimulated neurogenesis in vitro and neuron markers in particular but og olygodendrocyte markers and glia increased only in LD-1227-enriched medium. Taken together, these data suggest that LD-1227 is able to significantly protect the brain structure redox system to higher degree than DHA. Moreover, from in vitro study it appears that marine bioactive compound, through it wide array of small unsaturated fatty acids, phospholipids and neurotransmitter precursors, is likely to influence neuronal and glial lineage to act differently from a DHA-rich mixture. PMID:23034252

  1. CHINESE OF HUMANITY

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    A discussion of chinese curriculum of primary school under the background of new curriculum reform Mao xinjuan Feng haiying [Abstract] in recent years, Chinese learning received more and more attention by people article mainly from the national studies this course concepts, the curriculum reform of elementary school curriculum requirements and how to effective implementation of primary national studies course several aspects under the background of curriculum reform of Chinese primary curriculum the new school

  2. Effects of dietary prebiotic GroBiotic®- A on growth performance, plasma thyroid hormones and mucosal immunity of great sturgeon, Huso huso (Linnaeus, 1758)

    DEFF Research Database (Denmark)

    Adel, M.; Nayak, S.; Lazado, Carlo Cabacang;

    2016-01-01

    The present study was conducted to evaluate the effects of Grobiotic®-A, a commercial prebiotics, when administered in feed on the growth performance, plasma thyroid hormones and mucosal immunity of great sturgeon (Huso huso). The commercial prebiotic mixture was supplemented in the diets at four...... inclusion level of 1% and higher (2% group compared to the control). Inhibitory activity of the skin mucus against pathogens, particularly Streptococcus iniae and Yersinia ruckeri, was significantly improved following prebiotic feeding. Taken together, dietary inclusion of GroBiotic®-A promoted growth...

  3. Chinese Foods; Teacher's Handbook.

    Science.gov (United States)

    Huang, Joe, Ed.

    Different styles of Chinese cooking, traditional food items, cooking utensils, serving techniques, and the nutritional value of Chinese cooking are described in this teaching guide. Lesson plans for the preparation of simple dishes are presented. Recipes, a shopping guide to San Francisco's Chinatown, a guide to sources of supplies, and a…

  4. Confucius Teaching Chinese Abroad

    Institute of Scientific and Technical Information of China (English)

    TANG YUANKAI

    2010-01-01

    @@ On December 1, 2009, the Confucius Institute in Lyon, France, held a plaque-unveiling ceremony. Thomas Boutonnet, a Frenchman who has studied Chinese for 10 years in France and China and who is also an institute supervisor, said the institute would offer courses in Chinese language and culture covering legal, wade and cultural fields.

  5. FROM THE CHINESE PRESS

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    Equality and harmony are mainstream in Chinese marriage. The conclusion was made by a systematic investigation in 1996 on love and marriage relations between couples in Shanghai, Harbin, Guangdong, Gansu and other regions. Six thousand couples were surveyed in a multi-period, separated level probability sampling; the research was conducted by the study group, "Marriage quality during the period of Chinese social

  6. Say That in Chinese

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Demand for Chinese language learning is fueling all aspects of the market, most notably the textbook publication industry Alarge-scale series of Chinese lan-guage textbooks are to be pub-lished in the coming years jointly by the China International Publi

  7. On Developing Business Chinese.

    Science.gov (United States)

    Hong, Wei

    1996-01-01

    Examines the significance of foreign languages for business, particularly Business Chinese, in the 1990s; its curriculum requirements; and the impact of business languages on international business. The article proposes a developmental plan for Business Chinese at the college level including goals, course materials, learning activities, and…

  8. Chinese by Choice

    Science.gov (United States)

    Beem, Kate

    2008-01-01

    A 2004 College Board survey revealed that school districts around America wanted to offer Chinese, but finding qualified teachers was a problem, says Selena Cantor, director of Chinese Language and Culture Initiatives for the College Board. So last year, a new College Board program brought guest teachers from China to school districts in 31…

  9. Equilibria of Chinese Auctions

    DEFF Research Database (Denmark)

    Branzei, Simina; Forero, Clara; Larson, Kate;

    Chinese auctions are a combination between a raffle and an auction and are held in practice at charity events or festivals. In a Chinese auction, multiple players compete for several items by buying tickets, which can be used to win the items. In front of each item there is a basket, and the play...

  10. Modern Chinese History Studies

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Famous Foreign Scholars' Lecture Tours in China Around the May Fourth Movement and Changes in Chinese Intellectual Circles From 1919 to 1924, John Dewey and four other famous foreign scholars came to China on lecture tours. These tours were great cultural undertakings to spread Western learning to the East after the First World War. The lectures these schol- ars gave helped to deepen the thoughts of Chinese people, and at the same time encouraged the diversification and evolution of Chinese intellectual circles. Firstly, the lectures hastened the birth of a contemporary Chinese wave of reflection on mo- dernity, and provided a basis for the theoretical views and cultural appeals of Liang Qichao and other members of the socalled "Orient Culture Faction," thereby increasing the tension intrinsic to the development of the New Culture Movement and to the expansion of intellectual horizons in Chinese intellectual circles.

  11. Chinese Companies in Switzerland

    Directory of Open Access Journals (Sweden)

    Esther Kessler

    2014-10-01

    Full Text Available In recent years, some of China’s leading firms have made headlines with their European expansion, by either opening new facilities or by acquiring or merging with significant enterprises in Europe. The goal of this paper is to contribute to the existing literature by examining Chinese enterprises expanding into Switzerland. The study also allows some conclusions for Chinese companies entering Central and Eastern Europe. We analyze via interviews the motivations of Chinese companies to expand into Switzerland as well as their behavior and the impediments in their internationalization process. Our findings show that Chinese companies fail to take advantage of certain benefits of western economies (such as open information and stable rule of law. To move forward efficiently, they should develop competence in dealing systematically with readily available market information, building professional networks that recognize a separation between business life and personal life, and managing their Chinese and foreign employees in the foreign cultural environment.

  12. On Chinese Parody Translation

    Institute of Scientific and Technical Information of China (English)

    熊俊

    2013-01-01

    Chinese parody, as a traditional figure of speech, has captured more and more attention from scholars. The researches conducted up to date are inadequate in theorizing and exploring its translation. This paper, based on the comparative data analysis of Chinese parody translation examples in different types of texts, attempts to probe into the means about how to achieve the clos⁃est function equivalence in rendering Chinese parody under the guidance of Sociosemiotic Approach. It is found that the nature of Chinese parody translation is to achieve the closest natural equivalence or similarity in expressive function, informative func⁃tion, vocative function and aesthetic function in its equivalents in English. And it is suggested that borrowing, imitating, para⁃phrasing and adapting are effective strategies in translating Chinese parody.

  13. Study on Translating Chinese into Chinese Sign Language

    Institute of Scientific and Technical Information of China (English)

    徐琳; 高文

    2000-01-01

    Sign language is a visual-gestural language mainly used by hearing impaired people to communicate with each other. Gesture and facial expression are important grammar parts of sign language. In this paper, a text-based transfor mation method of Chinese-Chinese sign language machine translation is proposed.Gesture and facial expression models are created. And a practical system is im plemented. The input of the system is Chinese text. The output of the system is "graphics person" who can gesticulate Chinese sign language accompanied by facial expression that corresponds to the Chinese text entered so as to realize automatic translation from Chinese text to Chinese sign language.

  14. Chinese nuclear insurance and Chinese nuclear insurance pool

    International Nuclear Information System (INIS)

    Chinese Nuclear Insurance Started with Daya Bay Nuclear Power Station, PICC issued the insurance policy. Nuclear insurance cooperation between Chinese and international pool's organizations was set up in 1989. In 1996, the Chinese Nuclear Insurance Pool was prepared. The Chinese Nuclear Insurance Pool was approved by The Chinese Insurance Regulatory Committee in May of 1999. The principal aim is to centralize maximum the insurance capacity for nuclear insurance from local individual insurers and to strengthen the reinsurance relations with international insurance pools so as to provide the high quality insurance service for Chinese nuclear industry. The Member Company of Chinese Nuclear Pool and its roles are introduced in this article

  15. Research in Ancient Chinese Language

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    JIANG Ji-cheng, A Brief Study of Arabian-Chinese Diaphone in Huihui Prescription Abstract: Based on meterials of Arabian- Chinese diaphone in Huihui Prescription, this paper studies all Chinese phonetic initials and finals in Yuan dynasty, including 21 initials and 34 finals. Key Words: Huihui Prescription, Arabian- Chinese diaphone, transferred sound, International Phonetic Alphabet

  16. White sturgeon mitigation and restoration in the Columbia and Snake Rivers upstream from Bonneville Dam: annual progress report: April 1999 - March 2000; ANNUAL

    International Nuclear Information System (INIS)

    We report on our progress from April 1999 through March 2000 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam. The study is a cooperative effort by the Oregon Department of Fish and Wildlife (ODFW; Report A), Washington Department of Fish and Wildlife (WDFW; Report B), U.S. Geological Survey Biological Resources Division (USGS; Report C), Columbia River Inter-Tribal Fish Commission (CRITFC; Report D), and the U.S. Fish and Wildlife Service (USFWS; Report E). This is a multi-year study with many objectives requiring more than one year to complete. Therefore, findings from a given year may be part of more significant findings yet to be reported. Highlights of results of our work from April 1999 through March 2000 are given

  17. White sturgeon mitigation and restoration in the Columbia and Snake Rivers upstream from Bonneville Dam; Annual progress report; April 1998-March 1999

    International Nuclear Information System (INIS)

    The authors report on their progress from April 1998 through March 1999 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam. The study is a cooperative effort by the Oregon Department of Fish and Wildlife (ODFW; Report A), Washington Department of Fish and Wildlife (WDFW; Report B), U.S. Geological Survey Biological Resources Division (USGS; Report C), U.S. Fish and Wildlife Service (USFWS; Report D), Columbia River Inter-Tribal Fish Commission (CRITFC; Report E), and the University of Idaho (UI; Report F). This is a multi-year study with many objectives requiring more than one year to complete. Therefore, findings from a given year may be part of more significant findings yet to be reported. Highlights of results of our work from April 1998 through March 1999 are given

  18. Hierarchical stochastic modeling of large river ecosystems and fish growth across spatio-temporal scales and climate models: the Missouri River endangered pallid sturgeon example

    Science.gov (United States)

    Wildhaber, Mark L.; Wikle, Christopher K.; Moran, Edward H.; Anderson, Christopher J.; Franz, Kristie J.; Dey, Rima

    2015-01-01

    We present a hierarchical series of spatially decreasing and temporally increasing models to evaluate the uncertainty in the atmosphere – ocean global climate model (AOGCM) and the regional climate model (RCM) relative to the uncertainty in the somatic growth of the endangered pallid sturgeon (Scaphirhynchus albus). For effects on fish populations of riverine ecosystems, cli- mate output simulated by coarse-resolution AOGCMs and RCMs must be downscaled to basins to river hydrology to population response. One needs to transfer the information from these climate simulations down to the individual scale in a way that minimizes extrapolation and can account for spatio-temporal variability in the intervening stages. The goal is a framework to determine whether, given uncertainties in the climate models and the biological response, meaningful inference can still be made. The non-linear downscaling of climate information to the river scale requires that one realistically account for spatial and temporal variability across scale. Our down- scaling procedure includes the use of fixed/calibrated hydrological flow and temperature models coupled with a stochastically parameterized sturgeon bioenergetics model. We show that, although there is a large amount of uncertainty associated with both the climate model output and the fish growth process, one can establish significant differences in fish growth distributions between models, and between future and current climates for a given model.

  19. Assessment of Chinese sturgeon habitat suitability in the Yangtze River (China): Comparison of generalized additive model, data-driven fuzzy logic model, and preference curve model

    Science.gov (United States)

    Yi, Yujun; Sun, Jie; Zhang, Shanghong; Yang, Zhifeng

    2016-05-01

    To date, a wide range of models have been applied to evaluate aquatic habitat suitability. In this study, three models, including the expert knowledge-based preference curve model (PCM), data-driven fuzzy logic model (DDFL), and generalized additive model (GAM), are used on a common data set to compare their effectiveness and accuracy. The true skill statistic (TSS) and the area under the receiver operating characteristics curve (AUC) are used to evaluate the accuracy of the three models. The results indicate that the two data-based methods (DDFL and GAM) yield better accuracy than the expert knowledge-based PCM, and the GAM yields the best accuracy. There are minor differences in the suitable ranges of the physical habitat variables obtained from the three models. The hydraulic habitat suitability index (HHSI) calculated by the PCM is the largest, followed by the DDFL and then the GAM. The results illustrate that data-based models can describe habitat suitability more objectively and accurately when there are sufficient data. When field data are lacking, combining expertise with data-based models is recommended. When field data are difficult to obtain, an expert knowledge-based model can be used as a replacement for the data-based methods.

  20. Success factors of Chinese restaurants

    OpenAIRE

    Aakala, Liwen Heli

    2009-01-01

    The objectives of the thesis fall into three aspects: 1) understanding Chinese entrepreneurship through some major aspects; such as, the characteristics of Chinese entrepreneur and successful skills needed; 2) scanning the Chinese culture that is associated with their entrepreneurial success in restaurant business as well as understanding the Finnish culture that affects Chinese restaurants’ presence in Finland; 3) acquainting with the competitive strategies that those Chinese restaurants emp...

  1. The Contrast of Chinese and English in the Translation of Chinese Poetry

    OpenAIRE

    Ning Li

    2009-01-01

    Chinese poetry is the soul of Chinese literature and Chinese culture. A good translation of a Chinese verse can promote the prevalence of Chinese culture. In the translation of Chinese poetry, translators should not only keep the characteristics of Chinese poems, but also embody the English characteristics. This article analyzed some versions of translation and proposed factors affecting the translation of Chinese poetry.

  2. Cultural Characteristics of Chinese Cuisine:From Contrastive Studies of English and Chinese

    Institute of Scientific and Technical Information of China (English)

    乞聪妮

    2014-01-01

    Chinese cuisine plays an important role in Chinese culture. The paper illustrates the features of Chinese cuisine in Chi-nese dish naming from different perspectives, and analyze them from contrastive studies of English and Chinese.

  3. Chinese Female Creativity

    Institute of Scientific and Technical Information of China (English)

    VALERIE; SARTOR

    2011-01-01

    "Many foreigners mistakenly believe that Chinese women are creatively oppressed,that they have been oppressed for centuries," Teacher Yang said,glancing at me wryly."That’s correct," I replied, lifting my eyebrows.

  4. Traditional Chinese Biotechnology

    Science.gov (United States)

    Xu, Yan; Wang, Dong; Fan, Wen Lai; Mu, Xiao Qing; Chen, Jian

    The earliest industrial biotechnology originated in ancient China and developed into a vibrant industry in traditional Chinese liquor, rice wine, soy sauce, and vinegar. It is now a significant component of the Chinese economy valued annually at about 150 billion RMB. Although the production methods had existed and remained basically unchanged for centuries, modern developments in biotechnology and related fields in the last decades have greatly impacted on these industries and led to numerous technological innovations. In this chapter, the main biochemical processes and related technological innovations in traditional Chinese biotechnology are illustrated with recent advances in functional microbiology, microbial ecology, solid-state fermentation, enzymology, chemistry of impact flavor compounds, and improvements made to relevant traditional industrial facilities. Recent biotechnological advances in making Chinese liquor, rice wine, soy sauce, and vinegar are reviewed.

  5. Chinese remainder codes

    Institute of Scientific and Technical Information of China (English)

    ZHANG Aili; LIU Xiufeng

    2006-01-01

    Chinese remainder codes are constructed by applying weak block designs and the Chinese remainder theorem of ring theory.The new type of linear codes take the congruence class in the congruence class ring R/I1 ∩ I2 ∩…∩ In for the information bit,embed R/Ji into R/I1 ∩ I2 ∩…∩ In,and assign the cosets of R/Ji as the subring of R/I1 ∩ I2 ∩…∩ In and the cosets of R/Ji in R/I1 ∩ I2 ∩…∩ In as check lines.Many code classes exist in the Chinese remainder codes that have high code rates.Chinese remainder codes are the essential generalization of Sun Zi codes.

  6. Chinese Remainder Codes

    Institute of Scientific and Technical Information of China (English)

    张爱丽; 刘秀峰; 靳蕃

    2004-01-01

    Chinese Remainder Codes are constructed by applying weak block designs and Chinese Remainder Theorem of ring theory. The new type of linear codes take the congruence class in the congruence class ring R/I1∩I2∩…∩In for the information bit, embed R/Ji into R/I1∩I2∩…∩In, and asssign the cosets of R/Ji as the subring of R/I1∩I2∩…∩In and the cosets of R/Ji in R/I1∩I2∩…∩In as check lines. There exist many code classes in Chinese Remainder Codes, which have high code rates. Chinese Remainder Codes are the essential generalization of Sun Zi Codes.

  7. Traditional Chinese biotechnology.

    Science.gov (United States)

    Xu, Yan; Wang, Dong; Fan, Wen Lai; Mu, Xiao Qing; Chen, Jian

    2010-01-01

    The earliest industrial biotechnology originated in ancient China and developed into a vibrant industry in traditional Chinese liquor, rice wine, soy sauce, and vinegar. It is now a significant component of the Chinese economy valued annually at about 150 billion RMB. Although the production methods had existed and remained basically unchanged for centuries, modern developments in biotechnology and related fields in the last decades have greatly impacted on these industries and led to numerous technological innovations. In this chapter, the main biochemical processes and related technological innovations in traditional Chinese biotechnology are illustrated with recent advances in functional microbiology, microbial ecology, solid-state fermentation, enzymology, chemistry of impact flavor compounds, and improvements made to relevant traditional industrial facilities. Recent biotechnological advances in making Chinese liquor, rice wine, soy sauce, and vinegar are reviewed. PMID:19888561

  8. Chinese Marine Materia Medica

    OpenAIRE

    Peter Proksch

    2014-01-01

    China is one of the first countries to use marine materia medica for treating diseases. Ancient books on Chinese herbology, such as Shennong Bencaojing (Shennong’s Classic of Materia Medica), Xinxiu Bencao (Newly Revised Materia Medica) and Bencao Gangmu (Compendium of Materia Medica), have detailed more than 110 marine herbs and thousands of marine herbal formulas (including those for Chinese food therapy). A great deal of information on marine herbs and their applications in medicine, colle...

  9. The Chinese Politeness Scale

    Institute of Scientific and Technical Information of China (English)

    王喜凤

    2012-01-01

    In order to make sense of what is said in an interaction,we have to look at various factors which relate to social distance and closeness.Generally,these factors include the specific situation language takes place,the relative status of the two participants,the message being delivered and finally the age of the participants.In this article,the Chinese Politeness Scale,based on Chinese social values and tradition,will be explained and demonstrated in detail.

  10. Traditional Chinese Medicine

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    2009013 Clinical observation on treatment of active rheumatoid arthritis with Chinese herbal medicine. SHENG Zhenghe(盛正和), et al.Dept TCM, 5th Affili Hosp, Guangxi Med Univ, Guangxi 545001. Chin J Integr Tradit West Med 2008;28(11):990-993. Objective To study the efficacy and safety of Chinese drugs for expelling evil-wind, removing dampness, promoting blood circulation and invigorating yin in treating active rheumatoid arthritis (RA).

  11. Chinese New Year

    Institute of Scientific and Technical Information of China (English)

    王芳

    2005-01-01

    The Chinese New Year is now known as the Spnng Festival because it starts trom the beginning otspring. Though there are some sayings about its origin (起源), all agree that the word Nian, which inmodern Chinese means “year”, was originally the name of a beast (野兽) that started to eat people thenight before the beginning of a new year.

  12. Chinese Entrepreneurs Go Global

    OpenAIRE

    Daniel Zhou

    2012-01-01

    China may be on the tipping point of explosive global growth. In response to changes in the global economy and an economic slowdown domestically, hundreds of thousands of Chinese SMEs are being encouraged to “go global” by their central and local governments. To a Chinese company, going global requires the expansion of its existing business in other countries or the development of new ventures with partners operating in other countries. Explosive growth in China may be possible, but it will d...

  13. Country Review: Chinese Taipei

    OpenAIRE

    OECD

    2008-01-01

    This report, prepared by the Secretariat of the OECD was the basis for a peer review examination of Chinese Taipei at the OECD’s Global Forum on Competition on 9 February, 2006. Competition law in Chinese Taipei has been an important element of the program of economic reforms that moved the economy from centrally directed emphasis on manufacturing and exports to a market-driven emphasis on services and high technology. The competition law follows mainstream practice about restrictive agreemen...

  14. The Magic of Chinese

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    One of the world's oldest languages appears to have a vibrant futureClassical Greek and Latin, two languages that share an ancient history with Chinese, have been threatened with extinction, being used primarily in classic books or for special purposes. Chinese, on the other hand, is thriving as more and more people develop an interest in learning the language, and its charm has been noticed by linguists.

  15. Chinese Advertising and Advanced Chinese Culture

    Institute of Scientific and Technical Information of China (English)

    Liu Fan

    2006-01-01

    @@ Chinese advertising has long been inseparable from the Chinese national culture from late Shang Dynasty and early Zhou Dynasty when Jiang Ziya beat sword to spread sound to the 21st century when the badge of Beijing 2008 Olympic Games sweeps the whole world. With cultural trait as one of its fundamental character,advertising naturally becomes one of the most important cultural industries in contemporary era. In recent years because of prevalent theme, "Rediscover the Brilliance of Ancient Cities," promoted by the 39th IAA World Congress and the 12th China Advertising Festival held in China, Beijing and Xi'an, two ancient cities, had been splendidly presented to the whole world.

  16. Breeding Plan to Preserve the Genetic Variability of the Kootenai River White Sturgeon, Final Report, December 1993.

    Energy Technology Data Exchange (ETDEWEB)

    Kincaid, Harold L.

    1993-11-01

    Natural reproduction in the Kootenai River white sturgeon population has not produced a successful year class since 1974, resulting in a declining broodstock and 20 consecutive year classes missing from the age-class structure. This report describes a captive breeding plan designed to preserve the remaining genetic variability and to begin rebuilding the natural age class structure. The captive breeding program will use 3--9 females and an equal number of males captured from the Kootenai River each spring. Fish will be spawned in pairs or in diallel mating designs to produce individual families that will be reared separately to maintain family identity. Fish will be marked to identify family and year class before return to the river. Fish should be returned to the river as fall fingerlings to minimize potential adaptation to the hatchery environment Initially, while tagging methods are tested to ensure positive identification after return to the river, it may be necessary to plant fish as spring yearlings. Number of fish planted will be equalized at 5,000 per family if fall fingerlings or 1,000 per family if spring yearlings. Assuming annual survival rates of 20% during the first winter for fall fingerling plants and 50% for years 1--3, and 85% for years 4--20 of all fish planted, the target numbers would yield 7.9 progeny per family or about 4 breeding pairs at age 20. Natural survival in the river environment during the 19+ years from planting to maturity would result in variability in genetic contribution of families to the next broodstock generation. Fish planted per family would be adjusted in future years when actual survival rate information is known. Broodfish will be tagged when captured to minimize multiple spawning of the same fish. implementation of this breeding plan each year for the 20-year generation interval, using 5 different mating pairs each year, will yield an effective population size of 200, or 22.5% of the estimated 1990 population.

  17. Chinese boxes: "Typhoon" and Conrad's history of the Chinese

    OpenAIRE

    Kerr, D.

    2009-01-01

    This essay examines the novel "Typhoon," by Joseph Conrad, as a story about Chinamen coolies and seamen onboard a steamer transporting Chinese laborers. It argues that the Chinese boxes of the coolies represent several versions of the history of the Chinese and that the coolies are also representatives of a civilization with ideas and institutions, a law and order of nature. It also describes the images of Chinese life and their association to sociality, cooperation, nourishment, simplicity, ...

  18. Valorització de la carn d'esturió

    OpenAIRE

    Prieto Navarro, Raquel

    2015-01-01

    Currently the average consumer makes decisions about the products they consume greatly from the organoleptic properties, causing them to be more demanding with regards to the sensory quality. The sturgeon (Acipenser baerii) is a fish that is associated with the production of caviar, but its meat is poorly understood by consumers. The aim of this paper is to describe the sensory quality of the meat produced in farmed sturgeon, using sensory analysis and instrumental measures, which should prov...

  19. Recovery of a US Endangered Fish

    OpenAIRE

    Mark B. Bain; Nancy Haley; Peterson, Douglas L.; Arend, Kristin K.; Mills, Kathy E.; Patrick J. Sullivan

    2007-01-01

    BACKGROUND: More fish have been afforded US Endangered Species Act protection than any other vertebrate taxonomic group, and none has been designated as recovered. Shortnose sturgeon (Acipenser brevirostrum) occupy large rivers and estuaries along the Atlantic coast of North America, and the species has been protected by the US Endangered Species Act since its enactment. METHODOLOGY/PRINCIPAL FINDINGS: Data on the shortnose sturgeon in the Hudson River (New York to Albany, NY, USA) were obtai...

  20. Evaluation of condition and technological performance of hybrid bester reared in standard and aquaponic system

    OpenAIRE

    Lorena Dediu; Victor Cristea; Ion Vasilean; Angelica Docan

    2011-01-01

    Bester sturgeon is considered a hybrid (cross between beluga Huso huso (Linnaeus, 1758)female and sterlet Acipenser ruthenus Linnaeus, 1758 male) with great potential for intensiveaquaculture technology due to its quality caviar and meat production. Apart from Russia, firstpromising experiments on sturgeon culture have been carried out in several European countries(Germany, Hungary, France) and Japan as well as the U.S.A. Yet, in the field of bester sturgeonnutrition many authors consider tha...

  1. Teaching Chinese Negotiating Style through Examination of Key Chinese Categories.

    Science.gov (United States)

    Myers, Dan

    This study examined different shades of meaning that a single word may have in Chinese in an effort to better understand the relationship between language and culture. An understanding of the exact meaning of Chinese words and expressions can greatly assist non-Chinese in understanding both the language and the society as a whole. A total of 102…

  2. Contemporary American Chinese Studies

    Institute of Scientific and Technical Information of China (English)

    Qiu Huafei

    2008-01-01

    The rise of modern American scholarship on China was largely attributed to the establishment of the American Joint Committee on Contemporary China (JCCC) in 1959 which sponsored all kinds of activities to promote Chinese studies, ranging from institutional support and financial resources to training courses. Since then, American study of China has entered into a period of sustainability that features academic and group-oriented research. It has become a mainstream discipline in American social science studies.1 There are some distinctive differences between early sinology and modern Chinese Studies: the latter is much more concentrated on the study of issues, comparative historical studies, and contemporary Chinese society. American Chinese studies stresses empirical research, textual data, and the application of theory to practice.Shanghai. He was a Fulbright visiting professor at State University of New York at Geneseo from 2006-2007. This treatise is one of a series of studies for China's National Research Foundation of Philosophy and Social Science (05BGJ012), "American Chinese Studies."

  3. History of Chinese medicinal wine.

    Science.gov (United States)

    Xia, Xun-Li

    2013-07-01

    Chinese medicinal wine is one type of a favorable food-drug product invented by Chinese ancestors for treating and preventing diseases, promoting people's health and corporeity, and enriching people's restorative culture. In the course of development of the millenary-old Chinese civilization, Chinese medicinal wine has made incessant progress and evolution. In different historical periods, Chinese medicinal wine presented different characteristics in basic wine medical applications, prescriptions, etc. There are many medical and Materia Medica monographs which have systemically and specifically reported on Chinese medicinal wine in past Chinese dynasties. By studying leading medical documents, this article made an outline review on the invention, development, and characteristics of Chinese medicinal wine. PMID:21853349

  4. Chinese Rebalancing and Australian Exports

    OpenAIRE

    Gerard Kelly

    2014-01-01

    The Chinese authorities plan to gradually rebalance the composition of Chinese economic growth from investment towards household consumption. This article uses the World Input-Output Database (WIOD) to give a general sense of how this rebalancing might affect Australian exports and economic activity. Dollar for dollar, Chinese investment appears to absorb more than twice as much Australian value-added output as Chinese household consumption. This largely reflects the significant role of resou...

  5. The influence of stocking density on growth performance and hematological profile of stellate sturgeon (A. stellatus, Pallas, 1771, reared in an industrial ,, flow-through’’ aquaculture system

    Directory of Open Access Journals (Sweden)

    Desimira Maria Dicu Stroe

    2013-10-01

    Full Text Available 800x600 The aim of this paper is to evaluate the influence of four different stocking densities, ranging from 18.7 to 28.6 kg m3, on growth performance and welfare of juvenile stellate sturgeons, reared in an industrial ,,flow-through’’ aquaculture system. At the end of 60 days experimental period, a survival rate of 98% and a stocking density that ranged from 32.5 to 39.8 kg m-3 were registered. The physiological state of biological material is characterized by the values ​​of hematological indices, that are not significant from the statistical point of view (p> 0.05, both at the beginning and at the end of experimental period, as well as between experimental variants. To assess the biological material growth performance, feed conversion ratio (FCR, specific growth rate (SGR and profile index were calculated. Regarding the growth performance parameters, better value are encountered at B1 (18.7 kg m-3 and B3 (23.3 kg m-3, appreciable values ​​at B2 (20.4 kg m-3 and low values at B4 (28.6 kg m-3. As conclusion, it can be assert that the initial stocking density of 28.6 kg m-3 is not optimal for rearing juvenile stellate sturgeons in an industrial ,,flow-through’’ aquaculture system. Normal 0 21 false false false RO X-NONE X-NONE MicrosoftInternetExplorer4

  6. Knowing Chinese character grammar.

    Science.gov (United States)

    Myers, James

    2016-02-01

    Chinese character structure has often been described as representing a kind of grammar, but the notion of character grammar has hardly been explored. Patterns in character element reduplication are particularly grammar-like, displaying discrete combinatoriality, binarity, phonology-like final prominence, and potentially the need for symbolic rules (X→XX). To test knowledge of these patterns, Chinese readers were asked to judge the acceptability of fake characters varying both in grammaticality (obeying or violating reduplication constraints) and in lexicality (of the reduplicative configurations). While lexical knowledge was important (lexicality improved acceptability and grammatical configurations were accepted more quickly when also lexical), grammatical knowledge was important as well, with grammaticality improving acceptability equally for lexical and nonlexical configurations. Acceptability was also higher for more frequent reduplicative elements, suggesting that the reduplicative configurations were decomposed. Chinese characters present an as-yet untapped resource for exploring fundamental questions about the nature of the human capacity for grammar. PMID:26684059

  7. Effects of Mitigative Measures on Productivity of White Sturgeon Populations in the Columbia River Downstream from McNary Dam: Determine Status and Habitat Requirements of White Sturgeon Populations in the Columbia and Snake Rivers Upstream from McNary Dam, 1997-1998 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ward, David L. (Oregon Department of Fish and Wildlife, Portland, OR)

    1999-02-01

    The authors report on their progress from April 1997 through March 1998 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam. The study is a cooperative effort by the Oregon Department of Fish and Wildlife (ODFW; Report A), Washington Department of Fish and Wildlife (WDFW; Report B), U.S. Geological Survey Biological Resources Division (USGS; Report C), National Marine Fisheries Service (NMFS; Report D), U.S. Fish and Wildlife Service (USFWS; Report E), and Columbia River Inter-Tribal Fish Commission (CRITFC; Report F). This is a multi-year study with many objectives requiring more than one year to complete. Therefore, findings from a given year may be part of more significant findings yet to be reported. Highlights of results of the work from April 1997 through March 1998 listed.

  8. Effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and determine the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam, 1997 Annual Report

    International Nuclear Information System (INIS)

    The authors report on their progress from April 1997 through March 1998 on determining the effects of mitigative measures on productivity of white sturgeon populations in the Columbia River downstream from McNary Dam, and on determining the status and habitat requirements of white sturgeon populations in the Columbia and Snake rivers upstream from McNary Dam. The study is a cooperative effort by the Oregon Department of Fish and Wildlife (ODFW; Report A), Washington Department of Fish and Wildlife (WDFW; Report B), U.S. Geological Survey Biological Resources Division (USGS; Report C), National Marine Fisheries Service (NMFS; Report D), U.S. Fish and Wildlife Service (USFWS; Report E), and Columbia River Inter-Tribal Fish Commission (CRITFC; Report F). This is a multi-year study with many objectives requiring more than one year to complete. Therefore, findings from a given year may be part of more significant findings yet to be reported. Highlights of results of the work from April 1997 through March 1998 listed

  9. Supporting Chinese Speaking Skills Online

    Science.gov (United States)

    Stickler, Ursula; Shi, Lijing

    2013-01-01

    Chinese is considered a difficult language to learn by most Western learners, yet recently more and more people are learning Chinese, and increasingly teaching is delivered online. Due to the nature of Chinese and the complexity of online learning, research has not yet produced sufficient information on students' and teachers' interaction during…

  10. Chinese Colleges Need More Endowment

    Institute of Scientific and Technical Information of China (English)

    杨瑞

    2014-01-01

    In this paper I talk about the importance of increased college endowments. First I will introduce the limited financial situation of current Chinese colleges. Second, I will present an analysis on the financial reports of STU and Yale. Thirdly, I will describe the current Chinese College endowment situation. In conclusion I will present four suggestions for enhancing current Chinese college endowments.

  11. The Chinese in Children's Books.

    Science.gov (United States)

    New York Public Library, NY.

    This is a selective annotated list of children's books about China and the Chinese. Topics range from dynasties, the era of Western influence, emigration, revolution, to present day affairs. Most of the books have a Chinese setting, but some are about the Chinese in America. Listings are made under categories such as picture books, stories for…

  12. On Chinese Culture Curriculum Planning

    Science.gov (United States)

    Wang, Catherine

    2006-01-01

    The importance of cultural elements in foreign language teaching has been widely accepted in recent years. This applies particularly to the teaching of Chinese as a foreign language (TCFL) to non-native Chinese speakers at tertiary level in mainland China. However, there is no commonly accepted blueprint that defines the parts of Chinese culture…

  13. Concepts of Chinese Folk Happiness

    Science.gov (United States)

    Ip, Po Keung

    2011-01-01

    Discourses on Chinese folk happiness are often based on anecdotal narratives or qualitative analysis. Two traditional concepts of happiness popular in Chinese culture are introduced. The paper constructs a concept of Chinese folk happiness on basis of the findings of a scientific survey on the Taiwanese people regarding their concepts of…

  14. Chinese Studies and Beyond

    DEFF Research Database (Denmark)

    Brødsgaard, Kjeld Erik

    2013-01-01

    politics", "corporatism", "civil society", "fragmented authoritarianism", etc. (Brødsgaard, 1989; Guo 2013). This paper will survey these approaches in order to place the analysis of the contemporary Chinese politics and history in a comparative perspective. A survey of the field will remind us that......Many different conceptual approaches and models have been used to analyze contemporary Chinese history and politics. Some of the more commonly used include "totalitarianism", "two-line struggle", "clientelism", "tendency analysis", "political culture", "interest group politics", "bureaucratic...

  15. Chinese Literature,Anyone?

    Institute of Scientific and Technical Information of China (English)

    ALI; ALIZADEH

    2007-01-01

    Like many other ancient cultures,China possesses an impressive and celebrated literary heritage. The master poets of the medieval Tang Dynasty(618-907), for example, are rightfully known as some of the world’s best lyric poets; the adventures of the Monkey King and his company—as told in the classical Chinese narrative Journey to the West—have achieved a global following (in part due to TV adaptations and the like); and novels, short story collections and memoirs by expat Chinese authors living in the West have won major literary prizes and become international bestsellers.

  16. Chinese Workers' Real Demand

    Institute of Scientific and Technical Information of China (English)

    Li Zhen

    2010-01-01

    @@ A new generation different from their elders Cheap labor has built Chinas economic miracle.As China's economy has bounced back,wages have followed suit.But,for the new generation of Chinese migrant workers,wages are not enough to meet their needs.

  17. Targeting the Chinese Consumer

    Institute of Scientific and Technical Information of China (English)

    MICHAEL O'NEILL

    2006-01-01

    @@ China's consumer markets have developed an almost mythic status in recent years; the Holy Grail for Western retailers and manufacturers desperate to carve a position in a market of such huge dimensions. But understanding the Chinese consumer is no easy task,as many overseas companies have found to their peril.

  18. Chinese Festivals and Customs.

    Science.gov (United States)

    Green, Sandra Aili

    Traditional festivals and customs of the Chinese people are described in this publication which can be used with secondary level students. In the margins of the text are numbers which indicate slides and cultural objects that relate to the text. The text, however, can be used without the slides and objects. The following festivals are described:…

  19. Chinese Entrepreneurs Go Global

    Directory of Open Access Journals (Sweden)

    Daniel Zhou

    2012-02-01

    Full Text Available China may be on the tipping point of explosive global growth. In response to changes in the global economy and an economic slowdown domestically, hundreds of thousands of Chinese SMEs are being encouraged to “go global” by their central and local governments. To a Chinese company, going global requires the expansion of its existing business in other countries or the development of new ventures with partners operating in other countries. Explosive growth in China may be possible, but it will depend on an appropriate strategy for going global. For a country that has firmly established itself as an international manufacturing hub, going global requires a shift in its entrepreneurial capacity, which is the focus of this article. We first assess the current situation in China to understand its current entrepreneurial focus and capacity, as well as the impetus for change. Next, we contrast the Kirznerian and Schumpeterian views of entrepreneurship to illustrate that – to go global – Chinese entrepreneurs must shift from an emphasis on exploiting pricing inefficiencies (i.e., Kirznerian entrepreneurship to an emphasis on innovation (i.e., Schumpeterian entrepreneurship. Finally, we examine unique characteristics of the business environment and culture in China, which are likely to impact the ability of Chinese entrepreneurs to go global.

  20. Chinese Lacquer Art

    Institute of Scientific and Technical Information of China (English)

    ChengXiangjun

    2003-01-01

    Over the sweep of Chinese longstanding history,numerous treasures and heritages have been left over,among which the lacquer art is a brilliant one.China is the earliest country in the world using natural lacquer,In the early 1970s,archeologists unearthed a red lacquer wood bowl in an excavation in the

  1. Modelling Chinese Smart Grid

    DEFF Research Database (Denmark)

    Yuksel, Ender; Nielson, Hanne Riis; Nielson, Flemming;

    In this document, we consider a specific Chinese Smart Grid implementation and try to address the verification problem for certain quantitative properties including performance and battery consumption. We employ stochastic model checking approach and present our modelling and analysis study using...

  2. Chinese Borrowings in English

    Institute of Scientific and Technical Information of China (English)

    JU Li-li

    2014-01-01

    There are eight types of English word formation, which are widely used nowadays in English. Among them, Borrowings, as one of widely used types of English word formation, has drawn people's attention because many English words are borrowed from other languages, such as German, Latin. This article aims to demonstrate Chinese Borrowings in English from two aspects.

  3. FROM THE CHINESE PRESS

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    China Plans to Stabilize Population Growth by the ’30s of the Next Century On November 19, 1997, China’s State Councilor, Song Jian, revealed that China should be able to achieve control of population growth about thirty years into the next century, when the Chinese population reaches 1.5 to 1.6 billion.

  4. Why I Learn Chinese

    Institute of Scientific and Technical Information of China (English)

    Benedicte; Corbiere

    2013-01-01

    <正>Even today, I still ask myself why I am so fascinated with Chinese language, history, culture, films, photos and economic development. My interest in China was not a spur-of-the-moment move; instead, all sorts of lucky chances since my childhood made me irresistibly interested in this great and impressive country. I was born in

  5. Cataloging Pirated Chinese Books

    Science.gov (United States)

    Wang, Sze-Tseng

    1971-01-01

    Various types of pirated Chinese books are given with specific examples. The basic steps followed for the proper identification of these books is described, and remedies suggested in case the original is discovered after the book has been cataloged according to the information given by the book pirate. (13 references) (Author/NH)

  6. About Chinese Characters

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Some Chinese characters refer to natural phenomena andsubstances, such as "雨" yu (rain), "云" yun (clouds), "雪" xue (snow),"电" dian (lightning) and "雷" lei (thunder). The original form of "雨"was"(?)," in which"(?)" represents the cloud layer, and"(?)"symbolizes rain drops.

  7. Prediction of Secondary Structure and B Cell Epitope of GH Protein from Acipenser sinensis%中华鲟GH蛋白二级结构和B细胞抗原表位的预测

    Institute of Scientific and Technical Information of China (English)

    刘红艳; 杨东; 张繁荣; 余来宁

    2009-01-01

    [Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone (GH) protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A.sinensis as the base, the secondary structure of GH protein from A.sinensis was predicted by the method of Garnier-Robson, Chou-Fasman and Karplus-Schulz, and its cell epitope was predicted by the method of Kyte-Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18-23, 55-56, 67-73, 83-86, 112-114, 151-157 and 209-211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19-23, 51-71, 84-95, 128-139, 164-176 and 189-196 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [Conclusion] The research provided the basis for the preparation of monoclonal antibody of GH protein from A.sinensis and provided the reference for the discussion for the molecular regulation mechanism of A.sinensis.

  8. Analisis Homograf Aksara Cina pada Kamus Oxford Concise English- Chinese,Chinese-English

    OpenAIRE

    Nathania, Lenny

    2011-01-01

    Homograph characters exist in every language as well as in Chinese. The Chinese homograph characters are phenomena that often make misunderstood by learners in pronunciation and meaning interpretation. In this thesis with a title “Analisis Homograf Aksara Cina pada Kamus Oxford Concise English – Chinese, Chinese – English” (“Analysis Chinese Homophones characters in Oxford Concise English - Chinese, Chinese - English Dictionary”), focus on Chinese homograph characters from i...

  9. An Introduction to Chinese Society of Immunology

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    Chinese Society of Immunology (CSI) was founded in 1984. It has had over 5000 members, among whom 1000 are members of IUIS. There are six Chinese periodicals associated with the Society: Chinese Journal of Immunology, Immunological Journal, Current Immunology, Chinese Journal of Cellular and Molecular Immunology; Chinese Journal of

  10. An Introduction to Chinese Society of Immunology

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    Chinese Socicty of Immunology (CSI) was founded in 1984. It has had over 5000 members, among whom 1000 are members of IUIS. There are six Chinese periodicals associated with the Society: Chinese Journal of Immunology,Immunological Journal,Current Immunology,Chinese Journal of Cellular and Molecular Immunology,Chinese Journal of

  11. Chinese Experiences on Orthokeratology.

    Science.gov (United States)

    Xie, Peiying; Guo, Xi

    2016-01-01

    The prevalence of myopia in Chinese children has been rising each year. Research has shown that orthokeratology is a good method for controlling the progression of myopia. In this article, we review the current prevalence of myopia, the development and management of orthokeratology, and the myopia control methods used in China. A physical and health survey release indicated that the ratio of poor vision in every range of ages is still high in China. During the past 17 years, Chinese doctors have developed a standard fitting procedure and medical management of orthokeratology patients. This article also reviews the clinical studies in China for Ortho-K lenses, such as myopia control, effects and safety, corneal change, and lens design and fitting. Finally, we review the development of the International Academy of Orthokeratology Asia in China. PMID:26704137

  12. Photovoltaic for the Chinese

    International Nuclear Information System (INIS)

    China produces and exports about half of the photovoltaic cells made in the world. About 1000 Chinese enterprises work in the photovoltaic sector and the offer grows too fast to allow some enterprises to cope with lower and lower production costs. Research activities are a key element for the reduction of production costs but small companies can not usually sustain sufficient research. Economists foresee a strong reorganization of the sector: some enterprises will cease their photovoltaic activities, others will face financial difficulties, others will merge to make bigger companies. To make the demand bigger Chinese authorities are taking steps to develop the photovoltaic home market. The installed capacity of photovoltaic plants in China is expected to be somewhere between 11 GWc and 18 GWc in 2015, figures to be compared with only 0.9 GWc in 2010. (A.C.)

  13. Chinese Investment in Italy

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    At the 12th China International Fair for Investment and Trade (CIFIT) held on September 8-11 in Xiamen, Fujian Province, government officials and entrepreneurs from all over the world canvassed Chinese entrepreneurs and investors to invest in their countries. Foreign countries and regions rented 16,000 square meters of exhibition space, an increase of more than 50 percent from last year. Among the 74 participating countries and regions, more than 50 held seminars about their invest- ment environments. Besides the Caribbean countries and underdeveloped African nations that are actively attracting investment, developed countries such as the Untied States, Germany, France, Italy and Sweden also showed extraordinary enthusiasm in trying to win over Chinese investors. Beijing Review interviewed Marinella Loddo, Director of the Industrial Cooperation Division of the Italian Institute for Foreign Trade which is also known as the Italian Trade Commission (ICE).

  14. Making Chinese Money

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Dutch-based information provider expands in China to tap new market There are many secrets that foreign companies need to know before starting businesses in China.Wolters Kluwer knows a few of them. "You can never expect business success by introducing foreign products or services on an as-is basis in China.We need to provide something special to meet the demands of Chinese customers," said Shasha Chang,

  15. Making Chinese Money

    Institute of Scientific and Technical Information of China (English)

    LIU XINLIAN

    2011-01-01

    There are many secrets that foreign companies need to know before starting businesses in China.Wolters Kluwer knows a few of them."You can never expect business success by introducing foreign products or services on an as-is basis in China.We need to provide something special to meet the demands of Chinese customers," said Shasha Chang,Wolters Kluwer China CEO.

  16. Pollution Across Chinese Provinces

    OpenAIRE

    Catherine Yap Co; Fanying Kong; Shuanglin Lin

    2008-01-01

    We revisit the environmental Kuznets curve (EKC) hypothesis using 1987-1995 data for Chinese provinces. A comparison of off-sample (1996-2004) predictions to actual emissions indicates that more stringent rules are still needed to fight industrial (waste water and dust) pollution. Auxiliary regressions show that conditional on income, northern provinces have lower industrial waste water pollution; non-coastal and provinces with smaller secondary industry shares have lower industrial (waste wa...

  17. Chinese institutional investors’ sentiment

    OpenAIRE

    Kling, Gerhard; Gao, Lei

    2008-01-01

    We use daily survey data on Chinese institutional investors’ forecasts to measure investors' sentiment. Our empirical model uncovers that share prices and investor sentiment do not have a long-run relation; however, in the short-run, the mood of investors follows a positive feedback process. Hence, institutional investors are optimistic when previous market returns were positive. Contrarily, negative returns trigger a decline in sentiment, which reacts more sensitively to negative than positi...

  18. Actually existing Chinese matriarchy

    OpenAIRE

    Stafford, Charles

    2008-01-01

    The essays in this volume present contemporary anthropological perspectives on Chinese kinship, its historical complexity and its modern metamorphoses. The collection draws particular attention to the reverberations of larger socio-cultural and politico-economic processes in the formation of sociality, intimate relations, family histories, reproductive strategies and gender relations – and vice-versa. Drawing on a wealth of ethnographic material from the late imperial period and from cont...

  19. INTERNATIONALIZATION OF CHINESE EXECUTIVES

    OpenAIRE

    Lingfang Fayol-Song

    2012-01-01

    Over the last two decades, Chinese nationals have increasingly been employed by multinational companies (MNCs) operating in China taking positions previously occupied by foreign expatriates from investor countries. The development of local managers has therefore become crucial in the field of human resource management because the success of these companies depends greatly upon the ability and competence of their executive management class. The present paper addresses the issue of how to devel...

  20. Buddhist Activism and Chinese Modernity

    Directory of Open Access Journals (Sweden)

    Hung-yok Ip

    2015-02-01

    Full Text Available The history of modern Chinese Buddhism has begun to attract attention in recent years. Some scholars have done inspiring research as they unravel the integration of Buddhism into the highly secularized process of Chinese modernity by drawing on the repository of knowledge on modern China. While this special issue joins this exciting endeavor, it also uses Buddhism as a window to reflect on scholarship on Chinese modernity. Conceptually, this special issue presses scholars in the field of modern China to rethink the place of tradition in the course of modernity. Thematically we show the expansionist impulse of Chinese Buddhism: In addition to envisioning the geographical expansion of their religion, Chinese Buddhists have endeavored to enhance the significance of Buddhism in various dimensions of Chinese society in particular and human life in general.

  1. Transmitting Chinese Medicine

    Science.gov (United States)

    Scheid, Volker

    2015-01-01

    Historians of Chinese medicine acknowledge the plurality of Chinese medicine along both synchronic and diachronic dimensions. Yet, there remains a tendency to think of tradition as being defined by some unchanging features. The Chinese medical body is a case in point. This is assumed to have been formalised by the late Han dynasty around a system of internal organs, conduits, collaterals, and associated body structures. Although criticism was voiced from time to time, this body and the micro/macrocosmic cosmological resonances that underpin it are seen to persist until the present day. I challenge this view by attending to attempts by physicians in China and Japan in the period from the mid 16th to the late 18th century to reimagine this body. Working within the domain of cold damage therapeutics and combining philological scholarship, empirical observations, and new hermeneutic strategies these physicians worked their way towards a new territorial understanding of the body and of medicine as warfare that required an intimate familiarity with the body’s topography. In late imperial China this new view of the body and medicine was gradually re-absorbed into the mainstream. In Japan, however, it led to a break with this orthodoxy that in the Republican era became influential in China once more. I argue that attending further to the innovations of this period from a transnational perspective - commonly portrayed as one of decline - may help to go beyond the modern insistence to frame East Asian medicines as traditional. PMID:26869864

  2. Current Chinese bryological literature (4)

    OpenAIRE

    Cao, Tong; Zhang, Yuanming; Yu, Jin

    2000-01-01

    According to our collections of literature, about 400 scientific papers dealing with Chinese bryophytes have been published in China and abroad during 1990’s. Among these, more than 50 % were published in different scientific journals in China and often written in Chinese with English abstract, which are not well known and assessable for foreign bryologists. Therefore, in addition to previous Chinese literature I-III (Cao et al. 1990, Li et Zhang 1993, 1994), we present the fourth part of Chi...

  3. Introduction to Chinese natural language processing

    CERN Document Server

    Wong, Kam-Fai; Xu, Ruifeng; Zhang, Zheng-sheng

    2009-01-01

    This book introduces Chinese language-processing issues and techniques to readers who already have a basic background in natural language processing (NLP). Since the major difference between Chinese and Western languages is at the word level, the book primarily focuses on Chinese morphological analysis and introduces the concept, structure, and interword semantics of Chinese words.The following topics are covered: a general introduction to Chinese NLP; Chinese characters, morphemes, and words and the characteristics of Chinese words that have to be considered in NLP applications; Chinese word

  4. Chinese Stratgagem And Chinese Business Negotiating Behaviour: An Introduction to Ji

    OpenAIRE

    Fang, T.

    1995-01-01

    The paper aims to arrive at an understanding of Chinese business negotiating behaviour from a Chinese stratagem perspective. The Chinese concept li (stratagem) which has its roots in ancient Chinese military thought is introduced to fill the gap in scholarship. The author has proposed a "S-B Model" which interpretes the strategic patterns of Chinese business negotiating behaviour from The Thirty-Six Stratagems, an ancient Chinese stratagem treatise. The paper argues that Chinese stratagems ha...

  5. Achievement of Chinese Dream and Chinese Spirit of the Age Going out

    OpenAIRE

    Li, Jian; Kunming YAO

    2014-01-01

    Chinese dream is a spiritual power to achieve the Great Renaissance of the Chinese nation. In the process of Great Renaissance, we create the great spirit of Chinese nation. With the development of economy prosperity, China has become the second largest economic power in the world. The Chinese nation is undergoing the course of Great Renaissance of Chinese dream. The process of the evolution of Chinese dream is the cultivating process of Chinese spirit of the age. That is the evolution of Chi...

  6. Neuroanatomical markers of speaking Chinese.

    Science.gov (United States)

    Crinion, Jenny T; Green, David W; Chung, Rita; Ali, Nliufa; Grogan, Alice; Price, Gavin R; Mechelli, Andrea; Price, Cathy J

    2009-12-01

    The aim of this study was to identify regional structural differences in the brains of native speakers of a tonal language (Chinese) compared to nontonal (European) language speakers. Our expectation was that there would be differences in regions implicated in pitch perception and production. We therefore compared structural brain images in three groups of participants: 31 who were native Chinese speakers; 7 who were native English speakers who had learnt Chinese in adulthood; and 21 European multilinguals who did not speak Chinese. The results identified two brain regions in the vicinity of the right anterior temporal lobe and the left insula where speakers of Chinese had significantly greater gray and white matter density compared with those who did not speak Chinese. Importantly, the effects were found in both native Chinese speakers and European subjects who learnt Chinese as a non-native language, illustrating that they were language related and not ethnicity effects. On the basis of prior studies, we suggest that the locations of these gray and white matter changes in speakers of a tonal language are consistent with a role in linking the pitch of words to their meaning. PMID:19530216

  7. What Next for Chinese Football?

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Earlier this year, a crackdown on football match-fixing and gambling was launched in China, ending with the arrest of several high-ranking officials with the Chinese Football Association, referees, coaches and senior club executives. How can Chinese football come out from

  8. The chinese health care system

    DEFF Research Database (Denmark)

    Hougaard, Jens Leth; Østerdal, Lars Peter Raahave; Yu, Yi

    2011-01-01

    We describe the structure and present situation of the Chinese healthcare system and discuss its primary problems and challenges. We discuss problems with inefficient burden sharing, adverse provider incentives and huge inequities, and seek explanations in the structural features of the Chinese h...

  9. Why Do I Study Chinese

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    <正>Translator’s Note: The cross language year program-the Chinese Language Year in France and the French Language Year in China-initiated by Chinese and French leaders were held in 2011 and 2012 to promote cultural exchanges and further deepen the China-France comprehensive strategic partnership. Taking advantage of the opportunity, the CPAFFC and the

  10. Internationalization of Chinese Higher Education

    Science.gov (United States)

    Chen, Linhan; Huang, Danyan

    2013-01-01

    This paper probes into the development of internationalization of higher education in China from ancient times to modern times, including the emergence of international connections in Chinese higher education and the subsequent development of such connections, the further development of internationalization of Chinese higher education, and the…

  11. Chinese students' great expectations

    DEFF Research Database (Denmark)

    Thøgersen, Stig

    2013-01-01

    to interpret their own educational histories and prior experiences, while at the same time making use of imaginaries of 'Western' education to redefine themselves as independent individuals in an increasingly globalised and individualised world. Through a case study of prospective pre-school teachers preparing...... to study abroad, the article shows how personal, professional and even national goals are closely interwoven. Students expect education abroad to be a personally transformative experience, but rather than defining their goals of individual freedom and creativity in opposition to the authoritarian political...... system, they think of themselves as having a role in the transformation of Chinese attitudes to education and parent-child relations....

  12. Chinese Journalism Students

    DEFF Research Database (Denmark)

    Dombernowsky, Laura Møller

    2014-01-01

    As important providers of information, analysis of current events and debates, journalists are subject to high expectations regarding their professional values. Journalism is considered to be more than merely a career; it is construed as a profession that builds on personal commitment to serve...... society. This chapter is concerned with Chinese journalism students' self-perceptions and evaluations of journalistic performances in order to understand the professional values to which they adhere. The study is based on semi-structured in-depth interviews conducted in two periods in Spring 2011 and Fall...

  13. CHINESE COMMUNITY IN ECUADOR

    Institute of Scientific and Technical Information of China (English)

    MARIA JOSE CIFUENTES CERVANTES

    2011-01-01

    @@ The Republic Of Ecuador is located in the west coast of South America.It has a total area of 256.370 km2 and a population of approximately 14 million.Spanish is considered as the official language.The country is subdivided into 24 provinces with the capital city being Quito and the other major city being Guayaquil.Since the year 2000 US Dollar had been the official currency.Approximately 50,000 people from China live now in Ecuador.Although the Chinese community in Ecuador is not as large as those in Brazil and Peru, it has a strong economic and social weight in the country.

  14. The Chinese Tiger Mother

    OpenAIRE

    Jacek Hołówka

    2011-01-01

    In 2010 a book by Amy Chua: Battle Hymn of the Tiger Mother was published and it sparked a broad discussion among pedagogues and the open society about the factors determining educational success. Chua forms a simple and provocative thesis – the Chinese mothers are the best in the world because they don’t spoil their children, quickly introduce them into the adult culture, have high expectations of them, they are brusque and cold but they teach their children how to survive and be competitive...

  15. Chinese Food Heats Up

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Earlier this year,the vice chairman of industry body the China Cuisine Association,Yang Liu,expressed his bewilderment at the absence of a truly successful Chinese restaurant chain."In this ancient nation with a restaurant culture thousands of years old,home to the most delicious food in the world,it is a pity that you can find world-famous traditional dishes and delicious snacks,but a globally competitive restaurant chain is nowhere to be found,"he told Sanlian Life Weekly.

  16. The "Coop-Comp" Chinese Negotiation Strategy

    OpenAIRE

    T. Fang

    1997-01-01

    The purpose of this article is to explore the cultural roots of Chinese business negotiation strategy. The article argues that, Chinese culture, with its cooperative and competitive components, has bestowed upon Chinese negotiators a distinctive "coop-comp" negotiation strategy. The Chinese are culturally capable of negotiating both sincerely and deceptively at the international business negotiation table.

  17. Chinese migrants and forced labour in Europe

    OpenAIRE

    Yun, Gao

    2004-01-01

    Based on a literature survey and on an analysis of current Chinese law on trafficking. Examines the development of Chinese businesses in Europe and shows how harsh immigration policies have led to an increase in human trafficking. Investigates forced labour among Chinese migrants and examines Chinese legislation to combat trafficking.

  18. Multicultural Awareness for the Classroom: The Chinese.

    Science.gov (United States)

    Valbuena, Felix Mario; And Others

    This guide provides the teacher of multiethnic students with information and teaching resources on Chinese. An historical overview of China and the Chinese experience in America is presented in English and Chinese. Several lesson plans and classroom activities reviewing Chinese geography, holidays, legends, and stories are presented. (APM)

  19. Classification and Translation of Chinese Abbreviations

    Institute of Scientific and Technical Information of China (English)

    郭颖婷

    2014-01-01

    Chinese abbreviation, containing fewer words and delivering a wealth of information, is a vital component of Chinese language. But the tremendous differences between Chinese and English make it an arduous task to translate Chinese abbreviations into English. Based on the analyses of the structure and patterns of word-formation of Chinese abbreviations, it makes a classifi-cation of Chinese abbreviations, summarize the translation methods, and point out some attention points in translation. A system-atic analysis on the structure and classification of Chinese abbreviations will be beneficial to reduce the mistakes in its translation.

  20. Social Anxiety among Chinese People

    Directory of Open Access Journals (Sweden)

    Qianqian Fan

    2015-01-01

    Full Text Available The experience of social anxiety has largely been investigated among Western populations; much less is known about social anxiety in other cultures. Unlike the Western culture, the Chinese emphasize interdependence and harmony with social others. In addition, it is unclear if Western constructed instruments adequately capture culturally conditioned conceptualizations and manifestations of social anxiety that might be specific to the Chinese. The present study employed a sequence of qualitative and quantitative approaches to examine the assessment of social anxiety among the Chinese people. Interviews and focus group discussions with Chinese participants revealed that some items containing the experience of social anxiety among the Chinese are not present in existing Western measures. Factor analysis was employed to examine the factor structure of the more comprehensive scale. This approach revealed an “other concerned anxiety” factor that appears to be specific to the Chinese. Subsequent analysis found that the new factor—other concerned anxiety—functioned the same as other social anxiety factors in their association with risk factors of social anxiety, such as attachment, parenting, behavioral inhibition/activation, and attitude toward group. The implications of these findings for a more culturally sensitive assessment tool of social anxiety among the Chinese were discussed.

  1. Being Chinese or Being Different

    DEFF Research Database (Denmark)

    Zhang, Chun

    which way they use ‘Chinese-ness’, the participants are able to reflect on their experiences and manifest a willingness to adapt to another culture, in which their identity as CFL teachers are constructed. By the end of study, the combination of teaching culture of Chinese and Danish, in which......This study reports on a qualitative research that explores how three beginning native CFL teachers in a Danish university use ‘Chinese-ness’ and how these serve in explaining their own narratives, either through identifying with, or distancing themselves from. Early results show that no matter...

  2. European Union-Chinese Relations

    Directory of Open Access Journals (Sweden)

    Víctor Pou Serradell

    2003-09-01

    Full Text Available The present paper situates the relations between the European Union (EU and China in a double framework: the general framework of UE-Asian relations, on the one hand, and the ASEM (Asia-Europe Meeting process initiated in 1996, on the other hand. Likewise, it examines EU-Chinese relations in a specific way –including the relations of the most relevant member states of the EU with China–, the latest events that have occurred in EU-Chinese relations in the new international scenario following the 2001 terrorist attacks in the United States, and the future perspectives for EU-Chinese relations.

  3. Atopic disease in the Hong Kong Chinese.

    OpenAIRE

    Fung, Y. M.; Mayberry, J F; Rhodes, J.; Newcombe, R G

    1982-01-01

    One hundred and sixty Chinese men and 96 women resident in Hong Kong completed a questionnaire about atopic disease and their responses were compared with replies from 500 Britons. Asthma and hayfever were less common in the Chinese and this could not be attributed to medical awareness as the results were similar in Chinese surgical patients and Chinese medical students. The role of heredity and environment may be assessed by studying Chinese people who have moved to Britain.

  4. The Analysis of the Chinese Politeness Scale

    Institute of Scientific and Technical Information of China (English)

    付晓慧

    2007-01-01

    With the introduction of pragmatic studies, politeness has aroused great interest among Chinese scholars. Different cultures have different traditions and values, which bring out different understanding and application of politeness strategies. Great Chinese culture brings out the complicated Chinese politeness scale. A successful communication depends on politeness principle. Learning the Chinese politeness scale will make we know more about the Chinese culture. By researching this, we will perform better in communication.

  5. Chinese journals: a guide for epidemiologists

    OpenAIRE

    Fung Isaac CH

    2008-01-01

    Abstract Chinese journals in epidemiology, preventive medicine and public health contain much that is of potential international interest. However, few non-Chinese speakers are acquainted with this literature. This article therefore provides an overview of the contemporary scene in Chinese biomedical journal publication, Chinese bibliographic databases and Chinese journals in epidemiology, preventive medicine and public health. The challenge of switching to English as the medium of publicatio...

  6. TRADITIONAL CHINESE MEDICINE

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    930433 A study on relationship between hy-pothyroidism and deficiency of kidney YANG.ZHA Lianglun(查良伦),et al.lnstit Integr TCM& West Med,Shanghai Med Univ,Shanghai,200040.Chin J Integr Tradit & West Med 1993;13(4):202—204.Thirty—two cases of hypothyroidism causedby various factors were treated for one year withChinese medicinal herbs preparation“Shen Lutablet”(SLT)to warm and reinforce the KidneyYang.34 normal persons were studied as a con-trol group.After treatment with SLT,the clini-cal symptoms of hypothyroidism were markedlyimproved.Average serum concentration of totalT3,T4 increased significantly from 67.06±4.81

  7. CHINESE OF HUMANITY

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Study on the Application of Mass Media and English Han cui xia (ShanDong BinZhou medical college ShanDong YanTai 264003)Read and Write Periodica,vol.9, No.5,16,2012(ISSN1672-1578,in,Chinese ) Abstract: Rapid technological development of mass media and Internt chances of improvement. This paper analyzes the present situation of adult teaching and learning of English and the application of mass media and Internet in it in the hope of changing the present situation and improving the effect in order for adult learners to benefit more from it.. Key words: mass media; Internet; adult education; teaching and learning of English

  8. Predictors of beginning reading in Chinese and English: A 2-year longitudinal study of Chinese kindergartners

    OpenAIRE

    Ho, CSH; McBrideChang, C

    2005-01-01

    Ninety Chinese children were tested once at age 4 and again 22 months later on phonological-processing and other reading skills. Chinese phonological-processing skills alone modestly predicted Chinese character recognition, and English letter-name knowledge uniquely predicted reading of both Chinese and English 2 years later. Furthermore, concurrently measured phonological-processing skills in Chinese, but not English, accounted for unique variance in both English and Chinese word recognition...

  9. Chinese English” and Its Implication on C-E Translation of Chinese Specific Expressions

    OpenAIRE

    Yi Chen

    2010-01-01

    This paper attempts to clarify Chinese English and explore its origin, focusing on the important role of Chinese English resulted in the translation of expressions of Chinese specific expressions. In view of the important role of Chinese English in cross-cultural communication, the paper proposes that the translators should take a justified attitude to “Chinese English” rather than avoid it as unnecessary or bad English and .the expressions with Chinese peculiarity should be particularly heed...

  10. Chinese Family: Some New Trends

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    It is a familiar sight in many Chinese cities to witness a large team escorting a baby pram in public: mother, grandmothers, ayi (nan-ny), another ayi maybe, grandfathers quiet possible, and finally tither if you are lucky.

  11. Chinese semantic processing cerebral areas

    Institute of Scientific and Technical Information of China (English)

    SHAN Baoci; ZHANG Wutian; MA Lin; LI Dejun; CAO Bingli; TANG Yiyuan; WU Yigen; TANG Xiaowei

    2003-01-01

    This study has identified the active cerebral areas of normal Chinese that are associated with Chinese semantic processing using functional brain imaging. According to the traditional cognitive theory, semantic processing is not particularly associated with or affected by input modality. The functional brain imaging experiments were conducted to identify the common active areas of two modalities when subjects perform Chinese semantic tasks through reading and listening respectively. The result has shown that the common active areas include left inferior frontal gyrus (BA 44/45), left posterior inferior temporal gyrus (BA37); the joint area of inferior parietal lobules (BA40) and superior temporal gyrus, the ventral occipital areas and cerebella of both hemispheres. It gives important clue to further discerning the roles of different cerebral areas in Chinese semantic processing.

  12. What Next for Chinese Football?

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ Earlier this year, a crackdown on football match-fixing and gambling was launched in China, ending with the arrest of several high-ranking officials with the Chinese Football Association, referees, coaches and senior club executives.

  13. Globally Oriented Chinese Plastics Industry

    Institute of Scientific and Technical Information of China (English)

    Liao Zhengpin

    2004-01-01

    @@ Through continued endeavor and persistent opening to the whole world the Chinese plastics industry has been developed into a comprehensive industrial system that forms the basic material industries side by side with the steel, cement and the timber industry.

  14. Chinese culture and fertility decline.

    Science.gov (United States)

    Wu, C; Jia, S

    1992-01-01

    Coale has suggested that cultural factors exert a significant influence on fertility reduction; countries in the "Chinese cultural circle" would be the first to show fertility decline. In China, the view was that traditional Chinese culture contributed to increased population. This paper examines the nature of the relationship between Chinese culture and fertility. Attention was directed to a comparison of fertility rates of developing countries with strong Chinese cultural influence and of fertility within different regions of China. Discussion was followed by an explanation of the theoretical impact of Chinese culture on fertility and direct and indirect beliefs and practices that might either enhance or hinder fertility decline. Emigration to neighboring countries occurred after the Qing dynasty. Fertility after the 1950s declined markedly in Japan, Singapore, Hong Kong, South Korea, Taiwan, and mainland China: all countries within the Chinese cultural circle. Other countries within the Chinese circle which have higher fertility, yet lower fertility than other non-Chinese cultural countries, are Malaysia, Thailand, and Indonesia. Within China, regions with similar fertility patterns are identified as coastal regions, central plains, and mountainous and plateau regions. The Han ethnic group has lower fertility than that of ethnic minorities; regions with large Han populations have lower fertility. Overseas Chinese in East Asian countries also tend to have lower fertility than their host populations. Chinese culture consisted of the assimilation of other cultures over 5000 years. Fertility decline was dependent on the population's desire to limit reproduction, favorable social mechanisms, and availability of contraception: all factors related to economic development. Chinese culture affects fertility reduction by affecting reproductive views and social mechanisms directly, and indirectly through economics. Confucianism emphasizes collectivism, self

  15. LES FRAYÈRES POTENTIELLES DE L’ESTURGEON EUROPÉEN ACIPENSER STURIO L. 1758 DANS LE BASSIN GARONNE-DORDOGNE. MÉTHODES D’INVESTIGATION, ÉTAT ACTUEL ET PERSPECTIVES.

    Directory of Open Access Journals (Sweden)

    JEGO S.

    2002-04-01

    Full Text Available Dans le cadre du programme de restauration de l’esturgeon européen Acipenser sturio L. 1758, nous avons recensé et caractérisé l’ensemble des frayères potentielles de cette espèce sur les fleuves Garonne et Dordogne. L’objectif de ce travail était de réaliser un premier bilan sur l’état actuel de ces zones et d’évaluer leur possible utilisation comme frayère. Avant ce travail, les seules données disponibles étaient constituées par une liste de frayères et de zones de pêche de géniteurs répertoriées en 1952 et 1980 par enquêtes auprès des pêcheurs. Une analyse des données publiées sur les autres espèces d’esturgeons a permis de cerner les principales caractéristiques physiques d’une frayère d’esturgeons. En croisant les informations issues de ces 2 sources, historique et bibliographique, nous avons retenu une liste de sites qui ont ensuite été caractérisés lors de campagnes sur le terrain. Cette caractérisation a porté sur la bathymétrie, le courant, la nature du substrat, la position dans le paysage et les impacts anthropiques immédiats. Les résultats de cette étude ont montré que la majorité des 28 sites étudiés répondait aux caractéristiques d’une frayère d’esturgeon en terme de courant, profondeur et substrat. Ils pouvaient donc être considérés comme frayères potentielles et leur capacité d’accueil était suffisante à moyen terme. Néanmoins il apparaît nécessaire de protéger réglementairement l’ensemble de ces sites afin de les soustraire aux impacts négatifs d’activité anthropiques.

  16. The Chinese Housing Provident Fund

    OpenAIRE

    Richard J. Buttimer Jr; Anthony Yanxiang Gu; Tyler T. Yang

    2004-01-01

    The Chinese government has embarked upon an effort to reduce the number of tenants living in publicly owned housing. This is a significant challenge for any government, but may be especially so for a country where private homeownership is a new option. Out of concern that many of its citizens could not afford to purchase their housing units, the Chinese government created the Housing Provident Fund. This program, which is similar to housing fund programs in other countries such as Thailand an...

  17. Bulimia nervosa in the Chinese.

    Science.gov (United States)

    Schmidt, U

    1993-12-01

    Typical DSM-III-R bulimia nervosa with self-induced vomiting was found in 2 women of Hong Kong Chinese origin and a Chinese man from Malaysia. All 3 cases had a family history of obesity. In 2 of the cases a period of weight gain and in the third case frank obesity preceded the onset of the eating disorder. Cultural transition seemed to play an important part in the onset and maintenance of the eating disorder. PMID:8293034

  18. The art of Chinese management?

    OpenAIRE

    Schlevogt, Kai-Alexander.

    1998-01-01

    An empirical study was undertaken (a) to examine the characteristics, influencing factors, and effectiveness of a distinctive Chinese management model adopted by the newly founded private enterprises in mainland China, as well as (b) to test the "fit" notion in contingency theory. The study was based on a random sample of 124 standardized personal interviews with CEOs of Chinese industrial and service companies in Beijing and Shanghai. The standardized questionnaire mainly con...

  19. Characteristics of Chinese Driver Behavior

    OpenAIRE

    Li, J.

    2014-01-01

    The high growth rate of vehicle ownership and many novel drivers in China determine the special features of Chinese driver behavior. This thesis introduces a comparative study on driver behavior by the analysis of saturation flow at urban intersections, Driver Behavior Questionnaire surveys, focus group discussion, and in-car tests. The main characteristics of Chinese driver behavior have been identified. A new method is developed for a simulation model calibration based on the study results.

  20. Training in Chinese Small Firms

    OpenAIRE

    Dai, Zhejun

    2007-01-01

    The dissertation aims to review the currently predominant literature on the factors influencing the training in small business in the industrialized Western economies and to carry out empirical investigation on the determinants of training in Chinese small business and then analyze whether the Western-based literature is consistent with the practices of training in Chinese small firms. In addition, relevant problems with training in small business are presented and recommendations on how to r...

  1. Chinese investments in the EU

    Directory of Open Access Journals (Sweden)

    Haico EBBERS

    2010-12-01

    Full Text Available China’s investments in the European Union are much lower than what you may expect given the economic size of both entities. These relatively low investments in Europe are a combination of priority and obstacles. The priority for investments is clearly in Asia, Africa and Latin America. This regional pattern is heavily influenced by the need to solve the resource shortage in the medium and long term. The investments in Europe and the United States are mostly market seeking investments. Research specifically focused on Chinese M&A abroad comes to the same conclusion. The success rate of Chinese M&A abroad is much lower than what we see with respect to American or European investments abroad. In this paper, we examine why Chinese firms are facing more difficulties in the European Union than in other regions. The paper focuses on Chinese M&A as proxy for total foreign direct investments abroad. By looking at the factors that have been documented as influencing the level of M&A abroad, it becomes clear that Chinese firms in Europe are hindered by many factors. For example, the trade between China and the EU is relatively low, the institutional quality is lower compared to the United States, there is less experience with respect to Europe and relatively many deals relate to State Owned Enterprises (SOE which makes the deal sensitive. So it is logical that Chinese investments are not very high in Europe. However, the research makes clear that the obstacles for Chinese investments in Europe are disappearing step by step. In that sense, we expect a strong increase of Chinese investments in Europe in the future.

  2. Who Will Save Chinese Football?

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Like a drama unfolding in a riveting suspense novel, the quadrennial global football gala is becoming more and more compelling as the climax approaches. Billions of people are glued to the tube watching the World Cup in Germany, and Chinese football fans are following the countdown with the rest of the world. According to a poll by China Central Television, the national broadcaster, World Cup games have attracted combined viewers of at least 10 billion. For Chinese football enthusiasts, however, the joy ...

  3. Beneficial effect of a sturgeon-based bioactive compound on gene expression of tumor necrosis factor-alpha, matrix metalloproteinases and type-10 collagen in human chondrocytes.

    Science.gov (United States)

    Catanzaro, R; Marotta, F; Jain, S; Rastmanesh, R; Allegri, F; Celep, G; Lorenzetti, A; Polimeni, A; Yadav, H

    2012-01-01

    In the present study, we examined the effect of a marine bioactive compound containing high-purity caviar-derived DNA, collagen elastin and protein extracts from sturgeon (LD-1227, Caviarlieri, Laboratoires Dom, Switzerland) on IL-1beta-induced activation and production of TNFalpha and MMP-13 in human osteo-arthritis (OA) chondrocytes and intracellular signaling factors. Human chondrocytes were derived from OA cartilage and stimulated with IL-1beta. Gene expression of TNFalpha, MMP-13, MMP-1 and Col10A1 was measured by quantitative RT-PCR. TNFalpha protein in culture medium was determined using cytokine-specific ELISA. Western immunoblotting was used to analyze the MMP-13 production in the culture medium and the activation of NF-kB. DNA binding activity of NF-kB p65 was determined using a highly sensitive and specific ELISA. MMP-13 activity in the culture medium was assayed by gelatine zymography. LD-1227 significantly decreased IL-1beta-stimulated gene expression and production of TNFalpha, MMP-1, MMP-13 and Col10A1 in human chondrocytes. The inhibitory effect of LD-1227 on the IL-1beta-induced expression of these genes was mediated at least in part via suppression of NF-kB p65. These data show that LD-1227 can inhibit IL-1beta-induced proliferation and inflammatory reactions via inhibited activation of the transcription factor NF-kB pathway in human chondrocytes derived from OA patients. These novel pharmacological actions of LD-1227 on IL-1beta-stimulated human OA chondrocytes provide suggestions that this marine biology compound may inhibit cartilage degradation by suppressing IL-1beta-mediated activation and the catabolic response in human chondrocytes. PMID:23034253

  4. Study on the Processing TechnoIogy of DeI icious Minced Sturgeon Meat%美味鲟鱼肉丁的加工工艺研究

    Institute of Scientific and Technical Information of China (English)

    田其英

    2015-01-01

    The delicious minced meat is made of fresh sturgeon;take the sensory quality as the indicator,the product quality processed by improved process is superior to that by traditional process. The minced sturgeon meat made by improved process looks golden brown,and is neat,sweet, homogeneous and chewy, with moderate salty and strong fish flavor. Its physicochemical and microbiological indicators are also in accordance with the related product quality standards.%以新鲜鲟鱼为原料加工美味鲟鱼肉丁,先以产品感官品质为指标,比较得出改良工艺加工的产品品质明显优于传统工艺制得的产品。利用改良的加工工艺制得鲟鱼鱼肉丁外观呈金黄色,形体整齐,咸甜适中,质地均匀,有嚼劲,鱼香味显著。其理化指标和微生物指标均符合相关产品质量标准。

  5. Hydrodynamic Simulations of Physical Aquatic Habitat Availability for Pallid Sturgeon in the Lower Missouri River, at Yankton, South Dakota, Kenslers Bend, Nebraska, Little Sioux, Iowa, and Miami, Missouri, 2006-07

    Science.gov (United States)

    Jacobson, Robert B.; Johnson, Harold E., III; Dietsch, Benjamin J.

    2009-01-01

    The objective of this study was to assess the sensitivity of habitat availability in the Lower Missouri River to discharge variation, with emphasis on habitats that might support spawning of the endangered pallid sturgeon. We constructed computational hydrodynamic models for four reaches that were selected because of evidence that sturgeon have spawned in them. The reaches are located at Miami, Missouri (river mile 259.6-263.5), Little Sioux, Iowa (river mile 669.6-673.5), Kenslers Bend, Nebraska (river mile 743.9-748.1), and Yankton, South Dakota reach (river mile 804.8-808.4). The models were calibrated for a range of measured flow conditions, and run for a range of discharges that might be affected by flow modifications from Gavins Point Dam. Model performance was assessed by comparing modeled and measured water velocities. A selection of derived habitat units was assessed for sensitivity to hydraulic input parameters (drag coefficient and lateral eddy viscosity). Overall, model results were minimally sensitive to varying eddy viscosity; varying lateral eddy viscosity by 20 percent resulted in maximum change in habitat units of 5.4 percent. Shallow-water habitat units were most sensitive to variation in drag coefficient with 42 percent change in unit area resulting from 20 percent change in the parameter value; however, no habitat unit value changed more than 10 percent for a 10 percent variation in drag coefficient. Sensitivity analysis provides guidance for selecting habitat metrics that maximize information content while minimizing model uncertainties. To assess model sensitivities arising from topographic variation from sediment transport on an annual time scale, we constructed separate models from two complete independent surveys in 2006 and 2007. The net topographic change was minimal at each site; the ratio of net topographic change to water volume in the reaches at 95 percent exceedance flow was less than 5 percent, indicating that on a reach

  6. CHINESE OF HUMANITY

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    The Interactive Warm-up in Jiang Hua (Department of Foreign Language, Modern College of Northwest University, Xi'an 710130, China)Read and Write Perodica,vol.9,No.10,10,2012 (ISSN1672 -1578,in, Chinese) Abstract: Classroom teaching activities should be an interactive process, which includes not only interaction between teachers and students but also includes the interaction between students and learning materials and the interaction between students and design activities in class. These interaction processes will directly affect the students learning interest and the effect. So classroom interactions are very important. But how to present these interactions well and make them educational in the classroom is a question which needs considered carefully by teachers. Because of the warm-up is the key part for teachers to introduce the whole unit and stimulate the interests of students, this article mainly discusses the warming-up part of college English teaching, to improve the classroom interactions, and stimulate the students' study enthusiasm and the initiative. Key words: Interaction; Educational; Warm-up

  7. Social identity and self-esteem among Mainland Chinese, Hong Kong Chinese, British born Chinese and white Scottish children

    OpenAIRE

    Dai, Qian

    2013-01-01

    The Chinese community is the fastest growing non-European ethnic group in the UK, with 11.2% annual growth between 2001 and 2007. According to the National Statistics office (2005), there are over a quarter of a million Chinese in Britain. Compared to other ethnic minority groups, the Chinese group is socio-economically widespread, characterized by high academic achievements and high household income. It is estimated that there are about 30,000 Chinese immigrant children stu...

  8. A Primer on Chinese Music Instruments Released

    Institute of Scientific and Technical Information of China (English)

    SuSan

    2005-01-01

    Chinese traditional musical instruments, with a history of 8,000 years, are known for their diverse forms and types. The qualities, functions and materials of these instrunents reflect the unique aesthetic value of Chinese traditional music.

  9. Chinese Private Direct Investment and Overseas Chinese Network in Africa%Chinese Private Direct Investment and Overseas Chinese Network in Africa

    Institute of Scientific and Technical Information of China (English)

    Hong Song

    2011-01-01

    Based on extensive interviews in China and in Africa over 2 years, the present paper investigates Chinese private direct investment in Africa. Drawing on the Swedish Uppsala model, we explore two mian issues. First, do Chinese private enterprises follow t

  10. Consideration on Wavelet through Chinese Archaian Dialectics

    Directory of Open Access Journals (Sweden)

    Yuanzhi Wang

    2010-03-01

    Full Text Available Materialistic philosophy opens out universal law of things evolution. Chinese Yinyang theory is materialistic. Wavelet contain dialectic rule inevitably. As material mathematic technique, wavelet can be given a new look by Chinese archaian dialectics.

  11. "Uighur's Contributions to the Ancient Chinese Culture"

    OpenAIRE

    SHIMIN, Geng

    2010-01-01

    In this my paper I speak briefly on the contributions made by the Uighurs and their ancestors to the Chinese civilization. Of course, the Chinese people also made great contributions to the civilization of Uighur people

  12. Translating Chinese Zero Anaphoric Subjects into English.

    Science.gov (United States)

    Lee, Cher-leng

    1993-01-01

    Deals with a major difference between European languages and Chinese, namely the sparse use of anaphoric reference in Chinese. Suggests that the translator's way of rendering references will affect the interpretational potential of the text in the target language. (NKA)

  13. Traditional Chinese medicine for primary liver cancer

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    @@ Further progress has been made in the traditional Chinese medicine for primary liver cancer over the past few years, especially in the research of traditional Chinese medicine (TCM) treatment principle, improvement of therapeutic results and prolonging the survival.

  14. Chinese Foreign Direct Investment in Indonesia

    DEFF Research Database (Denmark)

    Gammeltoft, Peter; Tarmidi, Lepi T.

    2013-01-01

    In Indonesia, no systematic study of Chinese FDI has been undertaken to date. This paper contributes to filling this research gap and analyses the current composition as well as the historical evolution of Chinese FDI in Indonesia, relying on a survey conducted in 2008 among Chinese invested...... enterprises supplemented with key informant interviews, available official statistics and secondary data. Considering the evolution of Chinese investments in Indonesia over time, investments have evolved from being individual and isolated projects to acquiring more systemic properties. Chinese companies have...... acquired a broader sectoral presence in Indonesia and Chinese invested companies in, e.g., extractive or manufacturing activities can increasingly rely on complementary Chinese investments in logistics, travel, finance, etc. Where the local development effects are concerned, a picture emerges where Chinese...

  15. Developing classification indices for Chinese pulse diagnosis

    CERN Document Server

    Shu, Jian-Jun

    2014-01-01

    Aim: To develop classification criteria for Chinese pulse diagnosis and to objectify the ancient diagnostic technique. Methods: Chinese pulse curves are treated as wave signals. Multidimensional variable analysis is performed to provide the best curve fit between the recorded Chinese pulse waveforms and the collective Gamma density functions. Results: Chinese pulses can be recognized quantitatively by the newly-developed four classification indices, that is, the wave length, the relative phase difference, the rate parameter, and the peak ratio. The new quantitative classification not only reduces the dependency of pulse diagnosis on Chinese physician's experience, but also is able to interpret pathological wrist-pulse waveforms more precisely. Conclusions: Traditionally, Chinese physicians use fingertips to feel the wrist-pulses of patients in order to determine their health conditions. The qualitative theory of the Chinese pulse diagnosis is based on the experience of Chinese physicians for thousands of year...

  16. The liberalisation of Chinese financial markets

    Czech Academy of Sciences Publication Activity Database

    Semerák, Vilém

    London : Imperial College Press, 2015 - (Brown, K.), s. 309-326 ISBN 978-1-78326-454-4 Institutional support: RVO:67985998 Keywords : Chinese financial market s * Chinese financial system Subject RIV: AH - Economics

  17. Chinese Curriculum Design and Motivation of Chinese Background Students in Australian Tertiary Education

    OpenAIRE

    Shi Li

    2010-01-01

    This study explores the need for a change in the current Chinese curriculum for Chinese-background students at the University of Newcastle (UoN). It examines the motivation behind the learning behaviour of Chinese-background students enrolling in the discipline of Chinese at the UoN as well as Chinese curriculum design at other Australian universities. The data from two sources were collected and analysed, Chinese-background students in the Chinese discipline at the UoN and lecturers in the C...

  18. Mapping Chinese Literature as World Literature

    OpenAIRE

    Zhang, Yingjin

    2015-01-01

    In his article "Mapping Chinese Literature as World Literature" Yingjin Zhang revisits the challenge of mapping Chinese literature as world literature in three steps: 1) he delineates of positions of view as proposed by Western scholars who engaged in rethinking world literature(s) in the age of globalization, 2) evaluates consequences of such a new mapping for Chinese literature and tests a different set of "technologies of recognition" (Shih) in the context of Chinese versus Sinophone studi...

  19. Introduction to CRM Systems in Chinese SMEs

    OpenAIRE

    Huang, Xiaoting

    2015-01-01

    CRM (Customer Relationship Management) is an efficient management tool for an enterprise to improve the service level and customer relationships. However, CRM is not widely applied by Chinese SMEs. The implementation of CRM still faces some problems in Chinese CRM market. In this thesis is CRM systems applied in Chinese SMEs were discussed Chinese,including the current situation of CRM and what the SMEs need from a CRM system. In addition, it was discussed how to implement a CRM system in...

  20. Student Approaches to Learning Chinese Vocabulary

    OpenAIRE

    Fu, I-Ping P.

    2005-01-01

    This research focuses on the strategies that native English speakers use as they learn to speak and write Chinese vocabulary words in the first year of an elementary Chinese class. The main research question was: what strategies do native English-speaking beginning learners of Chinese use to learn Chinese vocabulary words in their speaking and writing? The study was conducted at a medium-sized comprehensive university in the Southeastern U.S. The study drew from concepts and theories in s...

  1. Chinese Consumers' Preferences for Livestock Products

    OpenAIRE

    Peng, Xuehua; Marchant, Mary A.; Qin, Xiang Dong; Zhuang, Jun

    2005-01-01

    This research surveyed over 784 Chinese consumers in Shanghai and Nanjing in 2001 and 2002, evaluating their preferences for livestock product attributes using ordered-probit models and factor analyses. Empirical results confirm the heterogeneity of Chinese livestock retail markets and suggest that livestock distributors should focus on Chinese female consumers and young consumers considering cooking convenience as a key attribute. Chinese consumers with higher incomes placed less importance ...

  2. The Lancaster Corpus of Mandarin Chinese.

    OpenAIRE

    McEnery, A. M.; Xiao, R. Z.

    2003-01-01

    The Lancaster Corpus of Mandarin Chinese (LCMC) addresses an increasing need within the research community for a publicly available balanced corpus of Mandarin Chinese. LCMC has been constructed as part of a research project undertaken by the Linguistics Department, Lancaster University. The corpus is designed as a Chinese match of the Freiburg-LOB Corpus of British English (FLOB), and, as such, will provide a valuable resource for contrastive studies between English and Chinese as well as a ...

  3. Traditional Chinese culture in modern Product Design

    OpenAIRE

    Wu, Qing

    2015-01-01

    This paper describes the sources and features of traditional Chinese culture. It also discusses the aesthetic thoughts of Chinese traditional culture. Chinese traditional culture has been illustrated broad and far-reaching impact on design since ancient time. In the context of globalization and the rapid development of science and technology, the difference between traditional design and modern product design should be explored. Chinese traditional culture cannot be totally absorbed. It is im...

  4. Chinese translation norms for 1429 English words

    OpenAIRE

    Wen, Yun; Walter J B van Heuven

    2016-01-01

    We present Chinese translation norms for 1429 English words. Chinese-English bilinguals (N=28) were asked to provide the first Chinese translation that came to mind for 1429 English words. The results revealed that 71% of the English words received more than one correct translation indicating the large amount of translation ambiguity when translating from English to Chinese. The relationship between translation ambiguity and word frequency, concreteness and language proficiency was investigat...

  5. Introduction to "Mediating Chineseness in Cambodia"

    OpenAIRE

    Addendum by Penny Edwards; Lorraine Paterson

    2012-01-01

    In 1981, social anthropologist William Willmott declared, “Today, no-one identifies themselves as Chinese in Kampuchea [Cambodia]” (1981:45). He certainly had the authority to publish such a statement. Having conducted sustained fieldwork on Chinese community formation in Cambodia from 1962 to 1963, Willmott offered an unprecedented examination of social structures, political organization, and patterns of identification among urban Chinese in his monographs, The Chinese in Cambodia (1967) and...

  6. Localization, Globalization, and Traveling Chinese Culture

    OpenAIRE

    Wang, Chengjun; Ma, Junhong

    2015-01-01

    In their article "Localization, Globalization, and Traveling Chinese Culture" Chengjun Wang and Junhong Ma discuss three main channels of the "traveling" of Chinese culture to the outside world. Focusing on the situation of bilingualism (i.e., Chinese and English) and the need of spreading Chinese literature and culture especially in the English-speaking world, Wang and Ma argue for "localization and globalization." Further, Wang and Ma argue that in order to narrow the gap of the cultural di...

  7. Informization Implementation for Chinese Retailers

    Institute of Scientific and Technical Information of China (English)

    ZHU Yan; LI Yan; QIAN Yu; CHEN Jianfeng; CHEN Jian

    2008-01-01

    Retailing is an important component of every country's economic system. The current status and developments in the informization of Chinese retail industry were investigated by using questionnaires and interviews to survey 139 retailers throughout China. The investigation shows that Chinese retailers are in the initial informization stage, and can be classified into different types with corresponding informization characteristics. In addition, the survey identified the key problems faced by retailers in the initial stage. Developments in the information technology field were analyzed to identify the key technologies that Chinese retailers should focus on during the informization process. The investigation also shows that the retailers have not arrived at a consensus about information technology adoption, and thus hesitate to use new information technologies, such as the radio frequency identification.

  8. L2 Chinese: Grammatical Development and Processing

    Science.gov (United States)

    Mai, Ziyin

    2016-01-01

    Two recent books (Jiang, 2014, "Advances in Chinese as a second language"; Wang, 2013, "Grammatical development of Chinese among non-native speakers") provide new resources for exploring the role of processing in acquiring Chinese as a second language (L2). This review article summarizes, assesses and compares some of the…

  9. Mamma Mia!Hits Chinese Stages

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    The Chinese version of Broadway’s musical sensation set to run for six months The Chinese version of Mamma Mia!,one of the most popular musicals in the world,has come to China,to demonstrate the charm of the musical to Chinese citizens in three major cities in 2011.

  10. NEW INSIGHT OF CHINESE LIVESTOCK CONSUMER

    OpenAIRE

    Qin, Xiang Dong; Peng, Xuehua; Marchant, Mary A.

    2002-01-01

    This paper overviews the development of Chinese livestock market. Based on a consumer preferences survey, a series of ordered probit models is developed to estimate Chinese consumers' preferences on the attributes of frozen and chilled livestock products. Keywords: Chinese livestock market, consumers' preferences, ordered probit model, attributes

  11. Saving Chinese-Language Education in Singapore

    Science.gov (United States)

    Lee, Cher Leng

    2012-01-01

    Three-quarters of Singapore's population consists of ethnic Chinese, and yet, learning Chinese (Mandarin) has been a headache for many Singapore students. Recently, many scholars have argued that the rhetoric of language planning for Mandarin Chinese should be shifted from emphasizing its cultural value to stressing its economic value since…

  12. Factors Influencing the Learning of Chinese Characters

    Science.gov (United States)

    Sung, Ko-Yin; Wu, Hsiao-Ping

    2011-01-01

    This survey study, which involved 108 language learners enrolled in first-year Chinese as a foreign language classrooms in the United States, intended to address the research questions, "What types of Chinese-character learning strategies do US learners use?" and "Do US learners' Chinese-character learning strategy use differ based on the…

  13. Of Course You Can Learn Chinese.

    Science.gov (United States)

    Wexler, Henrietta

    1980-01-01

    To master the subtleties of Chinese takes years, but most Americans can learn some basic spoken and written Chinese in a matter of weeks or months. A new phonic system, Pin Yin Romanizing System, tones, structure, and characters, and a comparison of Japanese and Chinese are discussed. (MLW)

  14. WEB BASED TRANSLATION OF CHINESE ORGANIZATION NAME

    Institute of Scientific and Technical Information of China (English)

    Yang Muyun; Liu Daxin; Zhao Tiejun; Qi Haoliang; Lin Kaiming

    2009-01-01

    A web-based translation method for Chinese organization name is proposed. After analyzing the structure of Chinese organization name, the methods of bilingual query formulation and maximum entropy based translation re-ranking are suggested to retrieve the English translation from the web via public search engine. The experiments on Chinese university names demonstrate the validness of this approach.

  15. A Brief Introduction to Chinese Biological Abstracts

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Chinese Biological Abstracts (CBA), a state-level indexing and abstracting journal published monthly, is jointly sponsored by the Library of the Chinese Academy of Sciences, the Shanghai Institutes for Biological Sciences as well as the Biological Information Network of the Chinese Academy of Sciences, published and distributed by the Shanghai Institutes for Biological Sciences, and approved by the State Scientific and Technological Commission.

  16. A Brief Introduction to Chinese Biological Abstracts

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Chinese Biological Abstracts sponsored by the Library, the Shanghai Institutes for Biological Sciences, the Biological Documentation and Information Network, all of the Chinese Academy of Sciences, commenced publication in 1987 and was initiated to provide access to the Chinese information in the field of biology.

  17. A Brief Introduction to Chinese Biological Biological

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Chinese Biological Abstracts sponsored by the Library, the Shanghai Institutes for Biological Sciences, the Biological Documentation and Information Network, all of the Chinese Academy of Sciences, commenced publication in 1987 and was initiated to provide access to the Chinese information in the field of biology.

  18. Adaptation Processes in Chinese: Word Formation.

    Science.gov (United States)

    Pasierbsky, Fritz

    The typical pattern of Chinese word formation is to have native material adapt to changed circumstances. The Chinese language neither borrows nor lends words, but it does occasionally borrow concepts. The larger cultural pattern in which this occurs is that the Chinese culture borrows, if necessary, but ensures that the act of borrowing does not…

  19. Writing Concepts in Chinese Writing Instruction.

    Science.gov (United States)

    Wang, Xia

    1994-01-01

    This study focuses on how Chinese writing instruction can cause negative interference for Chinese English-as-a-Second-Language students writing in English. The study shows that in Chinese writing, the main idea can be more general, as a theme, or specific, as a thesis statement. It can also come at the beginning or end of a paper, and the writer…

  20. The Rhetoric of Chinese Layoff Memos

    Science.gov (United States)

    Sisco, Lisa A.; Yu, Na

    2010-01-01

    In this analysis the authors introduce three memos announcing layoffs in Chinese companies. The three memos, translated from Chinese, are from: (1) Hewlett Packard China, an American company doing business in China; (2) UT Starcom, founded in China; and (3) Rizhao Steel, one of China's largest steel manufacturers. Comparing the Chinese and…

  1. How Do Chinese Enterprises Look at REACH?

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ The new European REACH (Registration, Evaluation, Authorization of Chemicals) regulation has come into force. As soon as the REACH white paper was issued, Chinese enterprises started to research the possible impacts of REACH and prepare to cope with them. How then do these Chinese enterprises look at REACH? Following are views of some Chinese enterprises exporting chemical products to the European Union.

  2. Scientific and Technical Chinese, Volume II. Glossary.

    Science.gov (United States)

    Kao, Kung-yi; And Others

    A composite English-to-Chinese glossary of all terms introduced in the individual lessons of "Scientific and Technical Chinese" is presented here. The appendices include lists of weights and measures and chemical elements, and a partial list of Chinese government organizations and research institutes related to science and technology. (AMH)

  3. Chinese Information Processing and Its Prospects

    Institute of Scientific and Technical Information of China (English)

    Sheng Li; Tie-Jun Zhao

    2006-01-01

    The paper presents some main progresses and achievements in Chinese information processing. It focuses on six aspects, I.e., Chinese syntactic analysis, Chinese semantic analysis, machine translation, information retrieval, information extraction, and speech recognition and synthesis. The important techniques and possible key problems of the respective branch in the near future are discussed as well.

  4. Problems of Textbook in Teaching Chinese Poetry.

    Science.gov (United States)

    Kuo, Ta-hsia

    It is proposed that teachers of Chinese expose their students to traditional critical views of Chinese poetics and criticism through careful selection of poems. This approach to language study is based on the assumption that the student may gain insight and appreciation of Chinese poetry as well as a feeling for the culture. Arguments favoring…

  5. Teaching Oral Chinese in the United States

    Science.gov (United States)

    Cheng, Chin-Chuan

    1976-01-01

    Because Chinese language textbooks were judged inadequate in teaching vocabulary dealing with everyday life in the U.S. and in China, new methods and materials were introduced into an oral Chinese course. Prepared discussion topics, Chinese films, soap operas, and shortwave radio broadcasts were used. (CHK)

  6. Chinese Brush Calligraphy Character Retrieval and Learning

    Science.gov (United States)

    Zhuang, Yueting; Zhang, Xiafen; Lu, Weiming; Wu, Fei

    2007-01-01

    Chinese brush calligraphy is a valuable civilization legacy and a high art of scholarship. It is still popular in Chinese banners, newspaper mastheads, university names, and celebration gifts. There are Web sites that try to help people enjoy and learn Chinese calligraphy. However, there lacks advanced services such as content-based retrieval or…

  7. Modern Chinese Punctuation and CSL Reading Pedagogy.

    Science.gov (United States)

    Mickel, Stan

    1988-01-01

    Provides a brief history of Chinese punctuation and detailed analysis of modern Chinese punctuation. Three tables show: 1) uniquely Chinese punctuation markers; 2) markers graphemically identical and functionally similar to Western markers; and 3) markers graphemically identical but functionally different from Western markers. Each punctuation…

  8. A Cultural Perspective on Chinese Politeness

    Institute of Scientific and Technical Information of China (English)

    Lu Xiaofang

    2011-01-01

    Politeness is a universal phenomenon, but its actual manifestations and the standards for judgment are culturally different. This paper analyzes the characteristics of Chinese politeness from a cultural perspective. It aims to improve our understanding of Chinese politeness and our knowledge of Chinese traditional culture and undertake interpersonal communication successfully.

  9. Accessing Chinese Resources on the Internet.

    Science.gov (United States)

    Chou, Yu-lan Margaret

    1995-01-01

    Discusses Internet resources on subjects related to the Chinese, both in English and Chinese: discussion groups; news; literature and linguistics; social studies; East Asian Studies programs, departments, and libraries; Chinese computer issues; cultural activities; weather; business; art; and entertainment. Hardware and software requirements…

  10. How Should We Revive Traditional Chinese Culture?

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    There has been a growing interest during the past year in the popularity of traditional Chinese culture, both domestically and globally. Currently, over 40 Confucius Institutes have been set up worldwide, focusing on promoting Chinese culture. Named after China's most eminent philosopher and the father of Confucianism, the Chinese

  11. Young Chinese ESL Children's Home Literacy Experiences.

    Science.gov (United States)

    Xu, Hong

    1999-01-01

    Describes home literacy experiences of six Chinese English-as-a-second-language kindergartners. Includes the parents' provision of literate home environments as well as children's functional use of Chinese and English and engagement in Chinese and English literacy activities. Indicates the diverse and cultural nature of the home literacy…

  12. Introduction to "Mediating Chineseness in Cambodia"

    Directory of Open Access Journals (Sweden)

    Addendum by Penny Edwards

    2012-09-01

    Full Text Available In 1981, social anthropologist William Willmott declared, “Today, no-one identifies themselves as Chinese in Kampuchea [Cambodia]” (1981:45. He certainly had the authority to publish such a statement. Having conducted sustained fieldwork on Chinese community formation in Cambodia from 1962 to 1963, Willmott offered an unprecedented examination of social structures, political organization, and patterns of identification among urban Chinese in his monographs, The Chinese in Cambodia (1967 and The Political Structure of the Chinese Community in Cambodia (1970. However, subsequent to his research, Chinese communities suffered terribly during the repression of the Lon Nol government between 1970 and 1975 and the atrocities of the Democratic Kampuchea regime. Willmott thus declared Chinese communities—and a willingness to identify as Chinese—destroyed. This understandably pessimistic vision turned out to be unfounded; the next extensive research done on Chinese in Cambodia by Penny Edwards and Chan Sambath in 1995 showed Chinese communities rebuilding. However, the descriptions of these communities showed a complexity of identity formation—from recent immigrants, “the raw Chinese,” to the five “traditional” Chinese dialect groups—that differed markedly from the indexes of identity applied by Willmott in his initial analysis. Academic ideas of how Chineseness should be configured had shifted and complicated; ascribing identity had become increasingly problematic...

  13. Analysis of chinese language learning motivation and cultural preservation of Chinese Indonesian high school students

    OpenAIRE

    Sugiato Lim

    2014-01-01

    This paper analyzes the Chinese Indonesian high school students their language and cultural preservation, also their motivation to keep learning Chinese. By related survey, this paper tries to find out more about how far the young generation of Chinese Indonesian retains their language and culture as well as their motivation to learn Chinese. The contents particularly concerns to the subjects of Chinese learning experience, motivation, mother language backgrounds, religious backgrounds, Chine...

  14. Indigenous Research on Chinese Management

    DEFF Research Database (Denmark)

    Li, Peter Ping; Leung, Kwok; Chen, Chao C.;

    2012-01-01

    We attempt to provide a definition and a typology of indigenous research on Chinese management as well as outline the general methodological approaches for this type of research. We also present an integrative summary of the four articles included in this special issue and show how they illustrate...... our definition and typology of indigenous research on Chinese management, as well as the various methodological approaches we advocate. Further, we introduce a commentary on the four articles from the perspective of engaged scholarship, and also three additional articles included in this issue...

  15. Brilliance Auto: Chinese, Global, Successful

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

      As the only automobile sponsor of BOAO FORUM FOR ASIA in April 2004, Brilliance Auto had a great opportunity to show her 'New Zhonghua" sedan which is an upper-medium car with completely independent intellectual property rights and repre sents the independent development philosophy of Brilliance Auto and is especially made for business personnel. The development pattern of Zhonghua sedan set a brand new model in Chinese auto industry to utilize the global resources and talents and international division of labor in the international arena, and she has become a typical sample of independent brand sedans in the contemporary Chinese auto industry.……

  16. Ikea success in chinese furniture

    OpenAIRE

    Yihong, Li

    2007-01-01

    This thesis will focus on the market exploiting and development of IKEA in China, analysis the characteristics of Chinese market and the supply-demand of the IKEA products in China. It also analyze the main Chinese consumers’ behaviour and evaluating the furniture retail market in China. IKEA is a successful case to open the china market recent years. The main goal is to acquire this information in order to provide the overseas retailers with a good starting point for creating an effective bu...

  17. Comment on Chinese tea culture

    Institute of Scientific and Technical Information of China (English)

    王俊人

    2015-01-01

    Drinking tea has become a part of people's daily life. Tea culture as the crystallization of Chinese tradition has a long history. China is the homeland of tea. Tea as one of important exports has made a great contribution to economic develop-ment of Ancient China. Tasting tea is a unique culture of China. The tea quality, teahouse’s atmosphere and tea wares are all considered to be very important when tasting. With a long time of the habit of drinking tea, Chinese people have given tea the special spirit:clearness, respect, joy and truthfulness.

  18. The globalisation of Chinese brands

    OpenAIRE

    Fan, Y.

    2006-01-01

    China has taken over Japan over the last decade to become the largest manufacturer and exporter of more than one hundred consumer products. However, China, as “the world factory”, has yet to create a single brand that is recognised worldwide. The recent acquisition of IBM’s PC business by China’s Lenovo may signal the beginning of the globalisation of Chinese brands. This paper considers the current brand revolution in China, focusing on the unique challenge faced by major Chinese enterprises...

  19. Chinese Ancient Football with Romanticism

    Institute of Scientific and Technical Information of China (English)

    江凌; 李晓勤

    2004-01-01

    Like other traditional Chinese sports, the ancient Chinese football, which used to be called “cuju”, has some differences from several sports in western countries concerning cultural and hamanist purport as well as metal aspiration, although it was similar with modern football to some extent, such as a leather-made ball with a bladder, rectangle sports ground, referee, goal and certain competitiveness. The author tries to talk about such difference in cultural and humanist purport as well as mental aspiration by making a comparison between “cuju” and modern football.

  20. Danish Chinese Center for Nanometals

    DEFF Research Database (Denmark)

    Winther, Grethe

    The Danish-Chinese Center for Nanometals is funded by the Danish National Research Foundation and the National Natural Science Foundation of China. The Chinese partners in the Center are Institute of Metal Research in Shenyang, Tsinghua University and Chongqing University. The Danish part of the...... Center is located at Risø DTU. The Center investigates metals, including light metals and steels, with internal length scales ranging from a few nanometers to a few micrometers. The structural morphologies studied are highly diverse, including structures composed of grain boundaries, twins and...

  1. The Chinese Tiger Mother

    Directory of Open Access Journals (Sweden)

    Jacek Hołówka

    2011-12-01

    Full Text Available In 2010 a book by Amy Chua: Battle Hymn of the Tiger Mother was published and it sparked a broad discussion among pedagogues and the open society about the factors determining educational success. Chua forms a simple and provocative thesis – the Chinese mothers are the best in the world because they don’t spoil their children, quickly introduce them into the adult culture, have high expectations of them, they are brusque and cold but they teach their children how to survive and be competitive. Chua shows this educational model as a contrast to the Western model, where the children have their own, naive and sentimental culture, their own shops and catwalks in shopping malls. The results of systematic research on education seem to prove something quite different. The educational success has to be measured using different scales, because it depends on different factors. The data published by OECD show that the level of education depends on the educational tradition of the society, level of GDP, intergenerational contacts, level of education of teachers and their social status. A strong determiner is the family, but not necessarily the mother. Even more, there is a strong correlation between the results in learning and a supporting stance of the parents, but also with their habit of spending free time with their children. The parents who take their children to the cinema, an a trip, gossip with them or take them to McDonalds, can be sure that their children will have statistically higher than the average grades. Detailed results from other sources show that success correlates the most with grades from previous class, parents’ habit of talking about things at school, higher education of the parents, being a child of a single mother, signing the child up for extracurricular classes from music and the mother working part-time. Failure correlates with being an Afro-American or Latino, checking homework by parents, free time after school without

  2. Approaches in Treating AIDS with Chinese Medicine

    Institute of Scientific and Technical Information of China (English)

    吕维柏

    2002-01-01

    @@ Editorial: AIDS is the archenemy of mankind, it also threatened Chinese people seriously. Although anti-retroviral drugs are effective, yet too expensive, the majority of 600 000 Chinese patients can't afford to purchase and use them. Decades ago, some scientists were trying to use Chinese Medicine in treating AIDS, experienc e gained but problems also exposed concerning strategy of research, methodol ogy, racial difference, Chinese drugs screening, integrative Chinese and western medicine, HIV and immunity, etc., sustained and integrative effort would bring safe, effective, and affordable drugs for the fatal pandemic all over the world

  3. The Evolvement and Development of Chinese Dragon

    OpenAIRE

    Zhu, Xiaoli

    2015-01-01

    Chinese Dragon is an art product exclusively owned by China. It is called an art product for it is a supernatural artistic creation which does not exist in the natural world. The image of Chinese Dragon is a combination of the ideal, the aspiration, the wisdom and the strength of Chinese people, and the development of history, it has formed several representative features and became the symbol of Chinese nation. In the folk artworks, the image of Chinese Dragon is expressed richly and in grea...

  4. Chinese Middle-Aged Confusion

    Institute of Scientific and Technical Information of China (English)

    CHENXINXIN

    2003-01-01

    THE middle-aged generation,those of the age 40 to 55, is special in China. Born in the 1950s and early 1960s, they grew up in the most difficult and chaotic periods of recent Chinese history, having experienced and suffered the Great Leap Forward at the end of the fifties and the “cultural revolution” ( 1966-1976).

  5. ENERGY CALCULUS IN CHINESE LANGUAGESEGMENTATION

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Based on cognitive science, the EnergyCalculus in Chinese language segmentation was presented to eliminate segmentation ambiguity. The notion of "EnergyCost" was advanced to denote the extent of the under-standability of a certain segmentation. EnergyCost function was defined with Z-notation. This approcah is effective to all natural language segmentation.

  6. Writing Quality Predicts Chinese Learning

    Science.gov (United States)

    Guan, Connie Qun; Perfetti, Charles A.; Meng, Wanjin

    2015-01-01

    To examine the importance of manual character writing to reading in a new writing system, 48 adult Chinese-as-a-foreign-language students were taught characters in either a character writing-to-read or an alphabet typing-to-read condition, and engaged in corresponding handwriting or typing training for five consecutive days. Prior knowledge of…

  7. The geography of Chinese science

    DEFF Research Database (Denmark)

    Andersson, David Emanual; Gunessee, Saileshsingh; Matthiessen, Christian Wichmann;

    2014-01-01

    . The geography of Chinese science is thus not only monocentric as regards overall scientific output, but also exhibits unusually hierarchical collaboration patterns. Unlike in Europe and North America, national and regional capitals are becoming ever more important as scientific coordination centers....

  8. Spoken Grammar for Chinese Learners

    Institute of Scientific and Technical Information of China (English)

    徐晓敏

    2013-01-01

    Currently, the concept of spoken grammar has been mentioned among Chinese teachers. However, teach-ers in China still have a vague idea of spoken grammar. Therefore this dissertation examines what spoken grammar is and argues that native speakers’ model of spoken grammar needs to be highlighted in the classroom teaching.

  9. Chinese President Meets Stephen Hawking

    Institute of Scientific and Technical Information of China (English)

    Fu Shuqin

    2002-01-01

    @@ Chinese President Jiang Zemin met on August 19 with Stephen Hawking and other world-renown scientists, including Shing-tung Yau, Edward Witten, D.Gross, A. Strominger, et al., who were attending an international conference on string theory and Prof.Hawking had just given a public speech titled "Brane New World" in Beijing.

  10. Ancient Chinese Bronzes: Teacher's Packet.

    Science.gov (United States)

    Smithsonian Institution, Washington, DC. Arthur M. Sackler Gallery.

    The focus of this teacher's packet is the bronze vessels made for the kings and great families of the early Chinese dynasties between 1700 B.C. and 200 A.D. The materials in the guide are intended for use by teachers and students visiting the exhibition, "The Arts of China," at the Arthur M. Sackler Gallery of the Smithsonian Institution in…

  11. 2006 Chinese RE Market Review

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Since 2005,China government established series of regulations to rectify Chinese rare earth industry.Especially,the notice of "ceasing the issue of new mining license and controlling the gross rare earth mining volume" implemented in April of 2006 largely reduced the mining in Southern China.

  12. Foreign Diplomats Cooking Chinese Dishes

    Institute of Scientific and Technical Information of China (English)

    Bai Yifeng

    2007-01-01

    @@ The 2nd Chinese Cuisine Contest for Foreign Diplomats(Shanghai),a large-scale activity as part of the 1st Shanghai Shopping Festival,was held at 15:30 on September 22 in Shanghai with the theme of "The Passionate Shanghai".

  13. Characteristics of Chinese Driver Behavior

    NARCIS (Netherlands)

    Li, J.

    2014-01-01

    The high growth rate of vehicle ownership and many novel drivers in China determine the special features of Chinese driver behavior. This thesis introduces a comparative study on driver behavior by the analysis of saturation flow at urban intersections, Driver Behavior Questionnaire surveys, focus g

  14. Chinese Sculpture as Public Art

    Institute of Scientific and Technical Information of China (English)

    YinShuangxi

    2003-01-01

    Chinese arts have undergone tremendous changes thanks to the fast-growing modernization and urbanization process in China. One of the monumental changes is the emergence of “public art”, most notably, urban sculpture. This art is increasingly related to modern urban planning, architecture, and community building.

  15. Chinese English Learners' Strategic Competence.

    Science.gov (United States)

    Wang, Dianjian; Lai, Hongling; Leslie, Michael

    2015-12-01

    The present study aims to investigate Chinese English learners' ability to use communication strategies (CSs). The subjects are put in a relatively real English referential communication setting and the analyses of the research data show that Chinese English learners, when encountering problems in foreign language (FL) communication, are characterized by the frequent use of substitution, approximation, circumlocution, literal translation, exemplification, word-coinage, repetition, and the infrequent use of cultural-knowledge and paralinguistic CSs. The rare use of paralinguistic strategies is found to be typical of Chinese English learners. The high frequency of literal translation, one first language (L1)-based strategy in our study sample, suggests that FL learners' use of L1-based CSs may depend more upon the developmental stage of their target language than the typology distance between L1 and the target language. The frequency of repetition reveals one fact that the Chinese English learners lack variety and flexibility in their use of CSs. Based on these findings, it was indicated that learners' use of CSs is influenced by a variety of factors, among which the development stage of their interlanguage and their cultural background are identified as two important factors. Some implications are finally suggested for the English foreign language teaching practice in China. PMID:25134668

  16. The Chinese Health Care System

    DEFF Research Database (Denmark)

    Hougaard, Jens Leth; Østerdal, Lars Peter; Yu, Yi

    In the present paper we describe the structure of the Chinese health care system and sketch its future development. We analyse issues of provider incentives and the actual burden sharing between government, enterprises and people. We further aim to identify a number of current problems and link...

  17. Clinical Service of Chinese Medicine

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ The clinical practice of traditional Chinese medicine (TCM) faces three major challenges:(1)How to enhance its contribution on overall medical service quality? (2) How to best address the unmet medical needs in the contemporary society? (3)How to guarantee that the traditional perspective for disease diagnosis and treatment not be neglected in clinical practice?

  18. Chinese Leadership Styles in Public Sector in Tianjin

    OpenAIRE

    Zhang, Nan

    2010-01-01

    Considerable studies on Chinese leadership styles apply Western models to test Chinese managers, and then define Chinese leadership style with Western ideas. This study assumes that Chinese managers have their own leadership styles distinctive from Westerners and tried to investigate the cultural and historical origin of Chinese leadership style, especially to explore how Confucianism have affected and shaped Chinese leadership style in modern Chinese society. This study adopted a qualitative...

  19. A Dialectal Chinese Speech Recognition Framework

    Institute of Scientific and Technical Information of China (English)

    Jing Li; Thomas Fang Zheng; William Byrne; Dan Jurafsky

    2006-01-01

    A framework for dialectal Chinese speech recognition is proposed and studied, in which a relatively small dialectal Chinese (or in other words Chinese influenced by the native dialect) speech corpus and dialect-related knowledge are adopted to transform a standard Chinese (or Putonghua, abbreviated as PTH) speech recognizer into a dialectal Chinese speech recognizer. Two kinds of knowledge sources are explored: one is expert knowledge and the other is a small dialectal Chinese corpus. These knowledge sources provide information at four levels: phonetic level, lexicon level, language level,and acoustic decoder level. This paper takes Wu dialectal Chinese (WDC) as an example target language. The goal is to establish a WDC speech recognizer from an existing PTH speech recognizer based on the Initial-Final structure of the Chinese language and a study of how dialectal Chinese speakers speak Putonghua. The authors propose to use contextindependent PTH-IF mappings (where IF means either a Chinese Initial or a Chinese Final), context-independent WDC-IF mappings, and syllable-dependent WDC-IF mappings (obtained from either experts or data), and combine them with the supervised maximum likelihood linear regression (MLLR) acoustic model adaptation method. To reduce the size of the multipronunciation lexicon introduced by the IF mappings, which might also enlarge the lexicon confusion and hence lead to the performance degradation, a Multi-Pronunciation Expansion (MPE) method based on the accumulated uni-gram probability (AUP) is proposed. In addition, some commonly used WDC words are selected and added to the lexicon. Compared with the original PTH speech recognizer, the resulting WDC speech recognizer achieves 10-18% absolute Character Error Rate (CER) reduction when recognizing WDC, with only a 0.62% CER increase when recognizing PTH. The proposed framework and methods are expected to work not only for Wu dialectal Chinese but also for other dialectal Chinese languages and

  20. Chinese traditional elements in the application of graphic design

    Institute of Scientific and Technical Information of China (English)

    曹倩倩

    2013-01-01

    Chinese traditional graphic art resources is extremely abundant, this article will from the Chinese tradi-tional culture elements are composed of the Chinese calligraphy, traditional auspicious patterns, national color three aspects, expounds the application of Chinese traditional elements in modern plane design, this paper expounds the application of Chinese traditional elements in modern plane design, research how to dig, change and reform.