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  1. Short tandem repeat technology has diverse applications: Individual identification, phylogenetic reconstruction and chimerism based post haematopoietic stem cell transplantation graft monitoring

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    Agrawal Suraksha

    2004-07-01

    Full Text Available BACKGROUND: Short Tandem Repeat (STR loci are widely considered to be effective for variety of applications including forensic applications, phylogenetic reconstruction and chimerism based post Haematopoietic Stem Cell Transplantation (HSCT graft monitoring. For each application, specific sets of STR loci are used. AIMS: In the present study, we have attempted to use same set of STR loci for varied purposes based on their efficacy and informativity. SETTINGS AND DESIGN: Population and patient based study. MATERIALS AND METHODS: We have analyzed 5 STR loci - vWA, Tho1, FES, F13 and TPOX in 1000 North Indians. All five markers were also analyzed for chimerism based graft monitoring after HSCT in 42 HLA matched pair of patient-donor to predict the outcome of transplantation. STATISTICAL ANALYSIS: The analysis was done for Hardy Weinberg equilibrium (HWE, Heterozygosity, Polymorphism information content (PIC and Power of Exclusion and Phylogenetic assessment. RESULTS AND CONCLUSIONS: High allelic variability in term of Heterozygosity (0.68-0.76, PIC (0.66-0.74 and high Power of exclusion (0.28-0.38 indicating high forensic utility. The ensuing PC plots finely resolved three basal clusters corresponding to three geo-ethnic groups of African, Orientals, and Caucasians. In post HSCT chimerism analysis, it was found that together these markers were informative in 38 pairs (98% and were able to predict the chimerism status successfully. There is a possibility that these STR loci along with forensic and phylogenetic importance, can predict the outcome of HSCT successfully.

  2. Short tandem repeat technology has diverse applications: Individual identification, phylogenetic reconstruction and chimerism based post haematopoietic stem cell transplantation graft monitoring

    OpenAIRE

    Agrawal Suraksha; Khan Faisal; Talwar Sudha; Nityanand Sonia

    2004-01-01

    BACKGROUND: Short Tandem Repeat (STR) loci are widely considered to be effective for variety of applications including forensic applications, phylogenetic reconstruction and chimerism based post Haematopoietic Stem Cell Transplantation (HSCT) graft monitoring. For each application, specific sets of STR loci are used. AIMS: In the present study, we have attempted to use same set of STR loci for varied purposes based on their efficacy and informativity. SETTINGS AND DESIGN: Population and patie...

  3. Chimerism Analysis of Cell-Free DNA in Patients Treated with Hematopoietic Stem Cell Transplantation May Predict Early Relapse in Patients with Hematologic Malignancies

    OpenAIRE

    Mahmoud Aljurf; Hala Abalkhail; Amal Alseraihy; Said Y. Mohamed; Mouhab Ayas; Fahad Alsharif; Hazza Alzahrani; Abdullah Al-Jefri; Ghuzayel Aldawsari; Ali Al-Ahmari; Belgaumi, Asim F.; Claudia Ulrike Walter; Hassan El-Solh; Walid Rasheed; Maher Albitar

    2016-01-01

    Background. We studied DNA chimerism in cell-free DNA (cfDNA) in patients treated with HSCT. Methods. Chimerism analysis was performed on CD3+ cells, polymorphonuclear (PMN) cells, and cfDNA using 16 small tandem repeat loci. The resulting labeled PCR-products were size-fractionated and quantified. Results. Analyzing samples from 191 patients treated with HSCT for nonneoplastic hematologic disorders demonstrated that the cfDNA chimerism is comparable to that seen in PMN cells. Analyzing leuke...

  4. Electroejaculation of chimeric rats

    OpenAIRE

    McCoy, Marina R.; Montonye, Daniel; Bryda, Elizabeth C.

    2013-01-01

    With the advent of genetic engineering of rodents came the need to assess fertility and germline competency, especially in chimeric rodents generated using embryonic stem cells. Traditional methods rely on natural mating and progeny testing, which is time- and cost-intensive. Electroejaculation is a faster method of collecting sperm for genetic analysis and offers the additional benefit of using fewer animals. This column describes a refined electroejaculation technique for chimeric rats usin...

  5. Identification and analysis of pig chimeric mRNAs using RNA sequencing data

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    Ma Lei

    2012-08-01

    Full Text Available Abstract Background Gene fusion is ubiquitous over the course of evolution. It is expected to increase the diversity and complexity of transcriptomes and proteomes through chimeric sequence segments or altered regulation. However, chimeric mRNAs in pigs remain unclear. Here we identified some chimeric mRNAs in pigs and analyzed the expression of them across individuals and breeds using RNA-sequencing data. Results The present study identified 669 putative chimeric mRNAs in pigs, of which 251 chimeric candidates were detected in a set of RNA-sequencing data. The 618 candidates had clear trans-splicing sites, 537 of which obeyed the canonical GU-AG splice rule. Only two putative pig chimera variants whose fusion junction was overlapped with that of a known human chimeric mRNA were found. A set of unique chimeric events were considered middle variances in the expression across individuals and breeds, and revealed non-significant variance between sexes. Furthermore, the genomic region of the 5′ partner gene shares a similar DNA sequence with that of the 3′ partner gene for 458 putative chimeric mRNAs. The 81 of those shared DNA sequences significantly matched the known DNA-binding motifs in the JASPAR CORE database. Four DNA motifs shared in parental genomic regions had significant similarity with known human CTCF binding sites. Conclusions The present study provided detailed information on some pig chimeric mRNAs. We proposed a model that trans-acting factors, such as CTCF, induced the spatial organisation of parental genes to the same transcriptional factory so that parental genes were coordinatively transcribed to give birth to chimeric mRNAs.

  6. Application of functional genomics to the chimeric mouse model of HCV infection: optimization of microarray protocols and genomics analysis

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    Smith Maria W

    2006-05-01

    Full Text Available Abstract Background Many model systems of human viral disease involve human-mouse chimeric tissue. One such system is the recently developed SCID-beige/Alb-uPA mouse model of hepatitis C virus (HCV infection which involves a human-mouse chimeric liver. The use of functional genomics to study HCV infection in these chimeric tissues is complicated by the potential cross-hybridization of mouse mRNA on human oligonucleotide microarrays. To identify genes affected by mouse liver mRNA hybridization, mRNA from identical human liver samples labeled with either Cy3 or Cy5 was compared in the presence and absence of known amounts of mouse liver mRNA labeled in only one dye. Results The results indicate that hybridization of mouse mRNA to the corresponding human gene probe on Agilent Human 22 K oligonucleotide microarray does occur. The number of genes affected by such cross-hybridization was subsequently reduced to approximately 300 genes both by increasing the hybridization temperature and using liver samples which contain at least 80% human tissue. In addition, Real Time quantitative RT-PCR using human specific probes was shown to be a valid method to verify the expression level in human cells of known cross-hybridizing genes. Conclusion The identification of genes affected by cross-hybridization of mouse liver RNA on human oligonucleotide microarrays makes it feasible to use functional genomics approaches to study the chimeric SCID-beige/Alb-uPA mouse model of HCV infection. This approach used to study cross-species hybridization on oligonucleotide microarrays can be adapted to other chimeric systems of viral disease to facilitate selective analysis of human gene expression.

  7. Electroejaculation of chimeric rats.

    Science.gov (United States)

    McCoy, Marina R; Montonye, Daniel; Bryda, Elizabeth C

    2013-06-01

    With the advent of genetic engineering of rodents came the need to assess fertility and germline competency, especially in chimeric rodents generated using embryonic stem cells. Traditional methods rely on natural mating and progeny testing, which is time- and cost-intensive. Electroejaculation is a faster method of collecting sperm for genetic analysis and offers the additional benefit of using fewer animals. This column describes a refined electroejaculation technique for chimeric rats using light gas anesthesia and a custom-made platform for sperm collection. PMID:23689457

  8. ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries.

    Science.gov (United States)

    Huang, Weiliang; Johnston, Wayne A; Boden, Mikael; Gillam, Elizabeth M J

    2016-02-01

    Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure-function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution that is well within the capability of most molecular biologists, but tools for the design and analysis of such experiments are limited. Here we introduce a suite of freely available online tools to make the construction and analysis of chimeric libraries readily accessible to the novice. REcut (http://qpmf.rx.umaryland.edu/REcut.html) facilitates the choice of DNA fragmentation strategy, while Xover (http://qpmf.rx.umaryland.edu/Xover.html) analyzes chimeric mutants to reveal recombination patterns and extract quantitative data. PMID:26842355

  9. Analysis of beta-globin mutations shows stable mixed chimerism in patients with thalassemia after bone marrow transplantation.

    Science.gov (United States)

    Kapelushnik, J; Or, R; Filon, D; Nagler, A; Cividalli, G; Aker, M; Naparstek, E; Slavin, S; Oppenheim, A

    1995-10-15

    Beta-thalassemia major (TM) is caused by any of approximately 150 mutations within the beta-globin gene. To establish the degree of chimerism after bone marrow transplantation (BMT), we have performed molecular analysis of beta-globin mutations in 14 patients with TM over a period of 10 years. All patients underwent T cell-depleted allogeneic BMT from HLA-identical related donors, using either in vitro T-cell depletion with CAMPATH 1M and complement or in vivo depletion using CAMPATH 1G in the bone marrow collection bag. To date, at different time periods after BMT, seven patients have some degree of chimerism; six of these patients, all blood transfusion-independent, have donor cells in the range of 70% to 95%, with stable mixed chimerism (MC). The seventh patient has less than 10% donor cells with, surprisingly, only minimal transfusion requirements. The detection of beta-globin gene point mutation, as used here, is a highly specific and sensitive marker for engraftment and MC in patients with thalassemia. In light of its specificity, the method is applicable in all cases of TM, as it is independent of sex and other non-globin-related DNA markers. The high incidence of MC found in our patients may be a consequence of the pre-BMT T-cell depletion. Because MC was associated with transfusion independence, complete eradication of residual host cells for effective treatment of TM and possibly other genetic diseases may prove not to be essential. PMID:7579421

  10. POST-RELIGION: TRADITIONALISTS’ ANALYSIS

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    Kirill M. Tovbin

    2013-12-01

    Full Text Available Aim of the study is to describe the phenomenon of post-religion as a specific spiritual sphere of the Post-modernity. Methodology. In the introduction analyzed a variety of methodological approaches, opening his inadequacy applied to the spiritual sphere of Post-modernity: «classic» religious studies, religious studies of traditional spiritual movements and the post-modern religious studies, partly produced Traditionalist school, could - according to the author's hypothesis - become a methodological basis for the most complete analysis of post-religion. Scientific novelty. In the main part of the article crystallized the authorized concept of post-religion, its relation with traditional and religious spiritual realms. Post-religion is positioned as the top of secularism enshrined in the departure from the straight and aggressive secularism of the Modernity, but without recourse to Tradition. Post-religion a simulation spirituality, skillfully imitating the outer areas of traditional spirituality and creates a planar religiosity, radically preventing attached to the vertical line of Traditions. In this regard, are the main artificially selected parameters of post-religion: deconstruction, splitting, virtualization, and collage. Deconstruction is a transformation of spirituality in semiotic set for egocentric selectivity of modern believer. Post-religion’s splitting is deprivation of spirituality center, destruction of sacral Center and the transformation of spirituality in the plane on which the intellectual and sensual wandering post-believer from one semiotic island to another. Virtualization is a displacement field of spiritual tension in a completely virtual area, isolated from the natural conditions of existence and created as his replacement, network discussion sites and galleries. Collage is an arbitrary combination of different semiotic pieces of Tradition with the aim of creating a believable picture of tradition; it is collage leads

  11. Chimerism Analysis of Cell-Free DNA in Patients Treated with Hematopoietic Stem Cell Transplantation May Predict Early Relapse in Patients with Hematologic Malignancies

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    Mahmoud Aljurf

    2016-01-01

    Full Text Available Background. We studied DNA chimerism in cell-free DNA (cfDNA in patients treated with HSCT. Methods. Chimerism analysis was performed on CD3+ cells, polymorphonuclear (PMN cells, and cfDNA using 16 small tandem repeat loci. The resulting labeled PCR-products were size-fractionated and quantified. Results. Analyzing samples from 191 patients treated with HSCT for nonneoplastic hematologic disorders demonstrated that the cfDNA chimerism is comparable to that seen in PMN cells. Analyzing leukemia patients (N = 126 showed that, of 84 patients with 100% donor DNA in PMN, 16 (19% had evidence of clinical relapse and >10% recipient DNA in the plasma. Additional 16 patients of the 84 (19% showed >10% recipient DNA in plasma, but without evidence of relapse. Eight patients had mixed chimerism in granulocytes, lymphocytes, and plasma, but three of these patients had >10% recipient DNA in plasma compared to PMN cells and these three patients had clinical evidence of relapse. The remaining 34 patients showed 100% donor DNA in both PMN and lymphocytes, but cfDNA showed various levels of chimerism. Of these patients 14 (41% showed laboratory or clinical evidence of relapse and all had >10% recipient DNA in cfDNA. Conclusion. Monitoring patients after HSCT using cfDNA might be more reliable than cellular DNA in predicting early relapse.

  12. Chimerism Analysis of Cell-Free DNA in Patients Treated with Hematopoietic Stem Cell Transplantation May Predict Early Relapse in Patients with Hematologic Malignancies

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    Aljurf, Mahmoud; Abalkhail, Hala; Alseraihy, Amal; Mohamed, Said Y.; Ayas, Mouhab; Alsharif, Fahad; Alzahrani, Hazza; Al-Jefri, Abdullah; Aldawsari, Ghuzayel; Al-Ahmari, Ali; Belgaumi, Asim F.; Walter, Claudia Ulrike; El-Solh, Hassan; Rasheed, Walid; Albitar, Maher

    2016-01-01

    Background. We studied DNA chimerism in cell-free DNA (cfDNA) in patients treated with HSCT. Methods. Chimerism analysis was performed on CD3+ cells, polymorphonuclear (PMN) cells, and cfDNA using 16 small tandem repeat loci. The resulting labeled PCR-products were size-fractionated and quantified. Results. Analyzing samples from 191 patients treated with HSCT for nonneoplastic hematologic disorders demonstrated that the cfDNA chimerism is comparable to that seen in PMN cells. Analyzing leukemia patients (N = 126) showed that, of 84 patients with 100% donor DNA in PMN, 16 (19%) had evidence of clinical relapse and >10% recipient DNA in the plasma. Additional 16 patients of the 84 (19%) showed >10% recipient DNA in plasma, but without evidence of relapse. Eight patients had mixed chimerism in granulocytes, lymphocytes, and plasma, but three of these patients had >10% recipient DNA in plasma compared to PMN cells and these three patients had clinical evidence of relapse. The remaining 34 patients showed 100% donor DNA in both PMN and lymphocytes, but cfDNA showed various levels of chimerism. Of these patients 14 (41%) showed laboratory or clinical evidence of relapse and all had >10% recipient DNA in cfDNA. Conclusion. Monitoring patients after HSCT using cfDNA might be more reliable than cellular DNA in predicting early relapse. PMID:27006832

  13. Immune Reconstitution Kinetics following Intentionally Induced Mixed Chimerism by Nonmyeloablative Transplantation.

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    Nayoun Kim

    Full Text Available Establishing mixed chimerism is a promising approach for inducing donor-specific transplant tolerance. The establishment and maintenance of mixed chimerism may enable long-term engraftment of organ transplants while minimizing the use of immunosuppressants. Several protocols for inducing mixed chimerism have been reported; however, the exact mechanism underlying the development of immune tolerance remains to be elucidated. Therefore, understanding the kinetics of engraftment during early post-transplant period may provide insight into establishing long-term mixed chimerism and permanent transplant tolerance. In this study, we intentionally induced allogeneic mixed chimerism using a nonmyeloablative regimen by host natural killer (NK cell depletion and T cell-depleted bone marrow (BM grafts in a major histocompatibility complex (MHC-mismatched murine model and analyzed the kinetics of donor (C57BL/6 and recipient (BALB/c engraftment in the weeks following transplantation. Donor BM cells were well engrafted and stabilized without graft-versus-host disease (GVHD as early as one week post-bone marrow transplantation (BMT. Donor-derived thymic T cells were reconstituted four weeks after BMT; however, the emergence of newly developed T cells was more obvious at the periphery as early as two weeks after BMT. Also, the emergence and changes in ratio of recipient- and donor-derived NKT cells and antigen presenting cells (APCs including dendritic cells (DCs and B cells were noted after BMT. Here, we report a longitudinal analysis of the development of donor- and recipient-originated hematopoietic cells in various lymphatic tissues of intentionally induced mixed chimerism mouse model during early post-transplant period. Through the understanding of immune reconstitution at early time points after nonmyeloablative BMT, we suggest guidelines on intentionally inducing durable mixed chimerism.

  14. Chimerism analysis in clinical practice and its relevance for the detection of graft rejection and malignant relapse in pediatric hematopoietic stem cell transplant patients.

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    Mellgren, Karin; Arvidson, Johan; Toporski, Jacek; Winiarski, Jacek

    2015-11-01

    Chimerism and clinical outcome data from 244 hematopoietic stem cell transplants in 218 children were retrospectively analyzed to assess their relevance for the detection of graft rejection and malignant relapse. Patients transplanted for a non-malignant disease had significantly higher proportions of residual recipient T cells in peripheral blood at one, three, and six months compared with patients transplanted for malignant disease. Recipient T-cell levels were below 50% at one month after transplantation in most patients (129 of 152 transplants). Graft rejection occurred more frequently in the group of patients with high levels of recipient cells at one month (10 graft rejections in the 23 patients with recipient T cells >50% at one month as compared to seven graft rejections occurred in 129 patients with recipient T cells <50% (p < 0.001). Multilineage chimerism data in 87 children with leukemia at one, three, and six months after transplantation were not correlated with subsequent relapse of malignant disease. In conclusion, early analysis of lineage-specific chimerism in peripheral blood can be used to identify patients who are at high risk of graft rejection. However, the efficacy of early chimerism analysis for predicting leukemia relapse was limited. PMID:26290161

  15. Quantitative chimerism kinetics in relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation

    Institute of Scientific and Technical Information of China (English)

    QIN Xiao-ying; WANG Jing-zhi; ZHANG Xiao-hui; LI Jin-lan; LI Ling-di; LIU Kai-yan; HUANG Xiao-jun; LI Guo-xuan; QIN Ya-zhen; WANG Yu; WANG Feng-rong; LIU Dai-hong; XU Lan-ping; CHEN Huan; HAN Wei

    2012-01-01

    Background Chimerism analysis is an important tool for the surveillance of post-transplant engraftment.It offers the possibility of identifying impending graft rejection and recurrence of underlying malignant or non-malignant disease.Here we investigated the quantitative chimerism kinetics of 21 relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation (HSCT).Methods A panel of 29 selected sequence polymorphism (SP) markers was screened by real-time polymerase chain reaction (RT-PCR) to obtain the informative marker for every leukemia patient.Quantitative chimerism analysis of bone marrow (BM) samples of 21 relapsed patients and 20 patients in stable remission was performed longitudinally.The chimerisms of BM and peripheral blood (PB) samples of 14 patients at relapse were compared.Results Twenty-one patients experienced leukemia relapse at a median of 135 days (range,30-720 days) after transplantation.High recipient chimerism in BM was found in all patients at relapse,and increased recipient chimerism in BM samples was observed in 90% (19/21) of patients before relapse.With 0.5% recipient DNA as the cut-off,median time between the detection of increased recipient chimerism and relapse was 45 days (range,0-120 days),with 76% of patients showing increased recipient chimerism at least 1 month prior to relapse.Median percentage of recipient DNA in 20 stable remission patients was 0.28%,0.04%,0.05%,0.05%,0.08%,and 0.05% at 1,2,3,6,9,and 12 months,respectively,after transplantation.This was concordant with other specific fusion transcripts and fluorescent in situ hybridization examination.The recipient chimerisms in BM were significantly higher than those in PB at relapse (P=0.001).Conclusions This SP-based RT-PCR essay is a reliable method for chimerism analysis.Chimerism kinetics in BM can be used as a marker of impending leukemia relapse,especially when no other specific marker is available.Based on our findings

  16. Analysis of serpin inhibitory function by mutagenesis of ovalbumin and generation of chimeric ovalbumin/PAI-2 fusion proteins.

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    McCarthy, B J; Worrall, D M

    1997-04-01

    Ovalbumin is a non-inhibitory serpin which lacks the ability to undergo the S --> R transition or conformational change. Amino acid residues in the hinge region (P11 to P14) of ovalbumin and other non-inhibitory serpins differ from the concensus sequence of this region of inhibitory serpins, and have been proposed to be responsible for lack of inhibitory properties, particularly the P14 charged residue. Site directed mutagenesis using PCR overlap extension was performed on these residues in ovalbumin to create a mutant with three amino acid changes, R340T, V342A and V343A. However analysis of the mutant recombinant ovalbumin with the consensus residues failed to show inhibitory activity or decreased stability, indicating that the hinge region alone is not responsible for lack of inhibition. A series of three fusion proteins were then constructed by replacing varying C-terminal regions of ovalbumin with the corresponding region of the inhibitory ov-serpin PAI-2 in order to further analyse serpin inhibitory function. Fusion proteins F1 and F2 contained approximately 16% and 35% PAI-2, respectively. This resulted in the replacing of structural features such as the reactive site loop, hinge region and beta sheet strands 5A and 6A. However both fusion proteins showed no inhibitory activity with the PAI-2 target protease urokinase (uPA) and no decrease in stability as analysed by transverse urea gradient (TUG) gels. The third chimeric fusion protein constructed (F3) contained 64% PAI-2 and did demonstrate inhibition of uPA, SDS-PAGE stable complex formation with uPA and increased instability on TUG gels. Structural differences between the inactive F2 and active F3 include the replacement of helix F and beta sheet strand 3A of ovalbumin with those of PAI-2, suggesting that these features may have a key role in serpin beta-sheet opening and inhibitory function. PMID:9126838

  17. Proteomic analysis of post-translational modifications

    DEFF Research Database (Denmark)

    Mann, Matthias; Jensen, Ole N

    2003-01-01

    Post-translational modifications modulate the activity of most eukaryote proteins. Analysis of these modifications presents formidable challenges but their determination generates indispensable insight into biological function. Strategies developed to characterize individual proteins are now syst...

  18. Cloning, purification, crystallization and preliminary X-ray analysis of a chimeric NADPH-cytochrome P450 reductase

    International Nuclear Information System (INIS)

    A 2.5 Å resolution data set was collected from a crystal of a soluble chimeric form of NADPH-cytochrome P450 reductase (CPR) produced using a fusion gene composed of the yeast FMN and the human FAD domains. The chimeric protein was crystallized in a modified conformation compared with the previously solved structures. NADPH-cytochrome P450 reductase (CPR) is the favoured redox partner of microsomal cytochromes P450. This protein is composed of two flavin-containing domains (FMN and FAD) connected by a structured linker. An active CPR chimera consisting of the yeast FMN and human FAD domains has been produced, purified and crystallized. The crystals belonged to the monoclinic space group C2 and contained one molecule per asymmetric unit. Molecular replacement was performed using the published rat and yeast structures as search models. The initial electron-density maps revealed that the chimeric enzyme had crystallized in a conformation that differed from those of previously solved structures

  19. Construction and analysis of variants of a polyvalent Lyme disease vaccine: approaches for improving the immune response to chimeric vaccinogens

    OpenAIRE

    Earnhart, Christopher G.; Marconi, Richard T.

    2007-01-01

    There is currently no Lyme disease vaccine commercially available for use in humans. Outer surface protein C (OspC) of the Borrelia has been widely investigated as a potential vaccinogen. At least 38 OspC types have been defined. While the antibody response to OspC is protective, the range of protection is narrow due to the localization of protective epitopes within OspC type-specific domains. To develop a broadly protective vaccine, we previously constructed a tetravalent chimeric vaccinogen...

  20. Functional analysis of chimeric genes obtained by exchanging homologous domains of the mouse mdr1 and mdr2 genes.

    OpenAIRE

    Buschman, E; Gros, P.

    1991-01-01

    A full-length cDNA clone for the mouse mdr1 gene can confer multidrug resistance when introduced by transfection into otherwise drug-sensitive cells. In the same assay, a full-length cDNA clone for a closely related member of the mouse mdr gene family, mdr2, fails to confer multidrug resistance. To identify the domains of mdr1 which are essential for multidrug resistance and which may be functionally distinct in mdr2, we have constructed chimeric cDNA molecules in which discrete domains of md...

  1. Blood chimerism in a dizygotic dichorionic pregnancy.

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    Jang, Ja-Hyun; Jung, Haiyoung; Kim, Jong-Hwa; Park, Won-Soon; Kim, Sun-Hee

    2010-10-01

    Blood chimerism in twins is known to occur through the transfer of hematopoietic stem cells between the fetuses via a common placenta. We present a case of blood chimerism in a dizygotic dichorionic twin pregnancy. The female twin was delivered at 34 weeks of gestation, and the male twin was stillborn. Pathologic examination confirmed dichorionic diamniotic placentas. The karyotype of the female child was obtained using peripheral blood sample, and it revealed a mixture of 46,XX and 46,XY cells (chi 46,XY[13]/46,XX[7]). FISH analysis performed on the buccal cells by using CEP X/Y probe (Abbott Molecular Inc., USA) revealed 100% XX signals (nuc ish Xcen(DXZ1x2)[500]). Gross examination of the external genitalia and abdominal ultrasonography revealed no definitive abnormal findings in relation to sex differentiation. When XX/XY chimerism is present in blood lymphocytes, careful examination of external genitalia and reproductive organs and further studies are required to detect chimerism in non-hematopoetic tissues. This is a rare case of blood chimerism in dichorionic placentas, in contrast to those in monochorionic placentas. PMID:20890086

  2. Chimeric Pestivirus Experimental Vaccines.

    Science.gov (United States)

    Reimann, Ilona; Blome, Sandra; Beer, Martin

    2016-01-01

    Chimeric pestiviruses have shown great potential as marker vaccine candidates against pestiviral infections. Exemplarily, we describe here the construction and testing of the most promising classical swine fever vaccine candidate "CP7_E2alf" in detail. The description is focused on classical cloning technologies in combination with reverse genetics. PMID:26458840

  3. Construction and analysis of variants of a polyvalent Lyme disease vaccine: approaches for improving the immune response to chimeric vaccinogens.

    Science.gov (United States)

    Earnhart, Christopher G; Marconi, Richard T

    2007-04-30

    There is currently no Lyme disease vaccine commercially available for use in humans. Outer surface protein C (OspC) of the Borrelia has been widely investigated as a potential vaccinogen. At least 38 OspC types have been defined. While the antibody response to OspC is protective, the range of protection is narrow due to the localization of protective epitopes within OspC type-specific domains. To develop a broadly protective vaccine, we previously constructed a tetravalent chimeric vaccinogen containing epitopes from OspC types A, B, K, and D. While this construct elicited bactericidal antibody against strains bearing each of the four OspC types, its solubility was low, and decreasing IgG titer to epitopes near the C-terminus of the construct was observed. In this report, construct solubility and immunogenicity were increased by dialysis against an Arg/Glu buffer. We also demonstrate the immunogenicity of the construct in alum. To further optimize epitope-specific immune responses, several constructs were generated with differing epitope organization or with putative C-terminal protective motifs. Analyses of murine antibody titers and isotype profiles induced by these constructs revealed that while the C-terminal tags did not enhance antibody titer, specific epitope reorganization and reiteration did. These analyses provide important information that can be exploited in the development of chimeric vaccinogens in general. PMID:17239505

  4. POST-PROCESSING ANALYSIS FOR THC SEEPAGE

    International Nuclear Information System (INIS)

    This report describes the selection of water compositions for the total system performance assessment (TSPA) model of results from the thermal-hydrological-chemical (THC) seepage model documented in ''Drift-Scale THC Seepage Model'' (BSC 2004 [DIRS 169856]). The selection has been conducted in accordance with ''Technical Work Plan for: Near-Field Environment and Transport: Coupled Processes (Mountain-Scale TH/THC/THM, Drift-Scale THC Seepage, and Post-Processing Analysis for THC Seepage) Report Integration'' (BSC 2004 [DIRS 171334]). This technical work plan (TWP) was prepared in accordance with AP-2.27Q, ''Planning for Science Activities''. Section 1.2.3 of the TWP describes planning information pertaining to the technical scope, content, and management of this report. The post-processing analysis for THC seepage (THC-PPA) documented in this report provides a methodology for evaluating the near-field compositions of water and gas around a typical waste emplacement drift as these relate to the chemistry of seepage, if any, into the drift. The THC-PPA inherits the conceptual basis of the THC seepage model, but is an independently developed process. The relationship between the post-processing analysis and other closely related models, together with their main functions in providing seepage chemistry information for the Total System Performance Assessment for the License Application (TSPA-LA), are illustrated in Figure 1-1. The THC-PPA provides a data selection concept and direct input to the physical and chemical environment (P and CE) report that supports the TSPA model. The purpose of the THC-PPA is further discussed in Section 1.2. The data selection methodology of the post-processing analysis (Section 6.2.1) was initially applied to results of the THC seepage model as presented in ''Drift-Scale THC Seepage Model'' (BSC 2004 [DIRS 169856]). Other outputs from the THC seepage model (DTN: LB0302DSCPTHCS.002 [DIRS 161976]) used in the P and CE (BSC 2004 [DIRS 169860

  5. Benefit of STR-based chimerism analysis to identify TA-GVHD as a cause of death: Utility of various biological specimens.

    Science.gov (United States)

    Raina, Anupuma; Chaudhary, Garima; Dogra, Tirath Das; Khandelwal, Deepchand; Balayan, Ajay; Jain, Vandana; Kanga, Uma; Seth, Tulika

    2016-04-01

    Transfusion-associated graft-versus-host disease (TA-GVHD) is a rare condition. It can occur after blood transfusion in immune-compromised and occasionally even in immune-competent patients, and is associated with a mortality rate of >90%. The diagnosis of TA-GVHD is often delayed because of its non-specific clinical features. A case of an immune-competent child who developed TA-GVHD is reported here. DNA profiling (short tandem repeat analysis), a technique that has a wide application in forensic medicine, was performed to detect the presence of donor cells in this patient. The findings suggest that more studies are needed with this tool, and the diagnostic potential of using other multiple biological specimens for DNA profiling such as the hair follicle and buccal swab should be evaluated. This is the first case report where the donor's DNA fingerprinting pattern was substantiated from a patient's hair follicle sample. Chimerism was also present in the blood and buccal swab specimens. PMID:25852093

  6. Peptidoglycan: a post-genomic analysis

    Directory of Open Access Journals (Sweden)

    Cayrou Caroline

    2012-12-01

    Full Text Available Abstract Background To derive post-genomic, neutral insight into the peptidoglycan (PG distribution among organisms, we mined 1,644 genomes listed in the Carbohydrate-Active Enzymes database for the presence of a minimal 3-gene set that is necessary for PG metabolism. This gene set consists of one gene from the glycosyltransferase family GT28, one from family GT51 and at least one gene belonging to one of five glycoside hydrolase families (GH23, GH73, GH102, GH103 and GH104. Results None of the 103 Viruses or 101 Archaea examined possessed the minimal 3-gene set, but this set was detected in 1/42 of the Eukarya members (Micromonas sp., coding for GT28, GT51 and GH103 and in 1,260/1,398 (90.1% of Bacteria, with a 100% positive predictive value for the presence of PG. Pearson correlation test showed that GT51 family genes were significantly associated with PG with a value of 0.963 and a p value less than 10-3. This result was confirmed by a phylogenetic comparative analysis showing that the GT51-encoding gene was significantly associated with PG with a Pagel’s score of 60 and 51 (percentage of error close to 0%. Phylogenetic analysis indicated that the GT51 gene history comprised eight loss and one gain events, and suggested a dynamic on-going process. Conclusions Genome analysis is a neutral approach to explore prospectively the presence of PG in uncultured, sequenced organisms with high predictive values.

  7. Analysis for post irradiation examination: (II)

    International Nuclear Information System (INIS)

    The Post Irradiation Examination Facility (PIEF) which is being constructed at Daeduk Engineering Center, Korea Advanced Energy Research Institute, and is supposed to be operated from the end of 1983 on schedule. This facility will be primarily concerned with the post irradiation examinations on fuel assemblies, fuel rods and the evaluation of reactor material properties. The contents of this report is the research of destructive examinations on nuclear fuels, reactor materials and components at hot laboratory. There are two major areas of investigation. 1. Metallographic examination on irradiated samples. 2. Mechanical and Physical property test on fuel rods and reactor materials. (Author)

  8. Chimeric enzymes with improved cellulase activities

    Science.gov (United States)

    Xu, Qi; Baker, John O; Himmel, Michael E

    2015-03-31

    Nucleic acid molecules encoding chimeric cellulase polypeptides that exhibit improved cellulase activities are disclosed herein. The chimeric cellulase polypeptides encoded by these nucleic acids and methods to produce the cellulases are also described, along with methods of using chimeric cellulases for the conversion of cellulose to sugars such as glucose.

  9. Interaction analysis of chimeric metal-binding green fluorescent protein and artificial solid-supported lipid membrane by quartz crystal microbalance and atomic force microscopy

    International Nuclear Information System (INIS)

    Non-specific adsorption and specific interaction between a chimeric green fluorescent protein (GFP) carrying metal-binding region and the immobilized zinc ions on artificial solid-supported lipid membranes was investigated using the quartz crystal microbalance technique and the atomic force microscopy (AFM). Supported lipid bilayer, composed of octanethiol and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine/1,2-dioleoyl-sn-glycero-3-[N- (5-amino-1-carboxypentyl iminodiacetic acid)succinyl] (NTA-DOGS)-Zn2+, was formed on the gold electrode of quartz resonator (5 MHz). Binding of the chimeric GFP to zinc ions resulted in a rapid decrease of resonance frequency. Reversibility of the process was demonstrated via the removal of metal ions by EDTA. Nanoscale structural orientation of the chimeric GFP on the membrane was imaged by AFM. Association constant of the specific binding to metal ions was 2- to 3-fold higher than that of the non-specific adsorption, which was caused by the fluidization effect of the metal-chelating lipid molecules as well as the steric hindrance effect. This infers a possibility for a further development of biofunctionalized membrane. However, maximization is needed in order to attain closer advancement to a membrane-based sensor device

  10. Post-Doctoral Researcher's Conceptions of Research: A Metaphor Analysis

    OpenAIRE

    Pitcher, Rod; Akerlind, Gerlese

    2009-01-01

    This paper uses the analysis of metaphors to study the conceptions of research held by a sample of post-doctoral researchers at five Australian universities. It is based on an analysis of the metaphors the researchers use in describing their research. The study produced three concepts that we have labelled ?research is exploring?, ?research is building? and ?research is living?. This study is unusual in its focus on post-doctoral researchers and the use of metaphors to identify their concepti...

  11. Pre/Post Data Analysis - Simple or Is It?

    Science.gov (United States)

    Feiveson, Al; Fiedler, James; Ploutz-Snyder, Robert

    2011-01-01

    This slide presentation reviews some of the problems of data analysis in analyzing pre and post data. Using as an example, ankle extensor strength (AES) experiments, to measure bone density loss during bed rest, the presentation discusses several questions: (1) How should we describe change? (2) Common analysis methods for comparing post to pre results. (3) What do we mean by "% change"? and (4) What are we testing when we compare % changes?

  12. An Analysis of the Service Structure of China Post

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The environment of the postal industry in China has experienced tremendous changes in the past few years. The environmental changes have some effects on the elements that decide the service structure of China Post. In such context, the current service structure of China Post needs some improvements to adapt itself to the changing environment. Aiming at the optimization of the service structure of China Post, this paper reviews the law of its development and makes an analysis of its current characteristics and the existing problems.

  13. T-Shaped Frame Critical and Post-Critical Analysis

    Directory of Open Access Journals (Sweden)

    Doicheva Albena

    2016-03-01

    Full Text Available The paper shows solution of a T-shaped frame, strength- ened with two linear springs, regarding critical and post-critical analysis. The solution is exact using the Euler elastic approach and the frame of reference, originated in the point of column axis inflexion. The derived Numerical results show the effect of the springs strengthening for the crit- ical and the post-critical system behaviour. The influence of the geometry change is analyzed, as well.

  14. Analysis of the atmospheric composition during the summer 2013 over the Mediterranean area using the CHARMEX measurements and the CHIMERE model

    Directory of Open Access Journals (Sweden)

    L. Menut

    2014-09-01

    Full Text Available The ADRIMED campaign provides measurements of all key parameters regarding atmospheric composition in the Mediterranean area during the summer 2013. This is an opportunity to quantify the ability of current models to adequately represent the atmospheric composition in this complex region, which is influenced by anthropogenic emissions from Europe, Africa, the Middle-East and from shipping activities as well as mineral dust emissions mostly from the arid areas in Africa, sea-salt emissions, biomass burning emissions and biogenic emissions from the vegetation. The CHIMERE model in its present version is a chemistry-transport model which takes into account all these processes. We show here by simulating the period from 5 June to 15 July 2013 with the CHIMERE model and comparing the results to both routine and specific ADRIMED measurements that this model allows an adequate representation the atmospheric composition over the western Mediterranean, in terms of ozone concentration, particulate matter (PM and aerosol optical depth (AOD. It is also shown that the concentrations of PM on all the considered area is dominated by mineral dust, even though local dust emissions in Europe are certainly overestimated by the model. A comparison with sulphate concentrations at Cape Corsica exhibits some discrepancies related to the regridding of shipping emissions.

  15. An integrative analysis of post-translational histone modifications in the marine diatom Phaeodactylum tricornutum

    KAUST Repository

    Veluchamy, Alaguraj

    2015-05-20

    Background: Nucleosomes are the building blocks of chromatin where gene regulation takes place. Chromatin landscapes have been profiled for several species, providing insights into the fundamental mechanisms of chromatin-mediated transcriptional regulation of gene expression. However, knowledge is missing for several major and deep-branching eukaryotic groups, such as the Stramenopiles, which include the diatoms. Diatoms are highly diverse and ubiquitous species of phytoplankton that play a key role in global biogeochemical cycles. Dissecting chromatin-mediated regulation of genes in diatoms will help understand the ecological success of these organisms in contemporary oceans. Results: Here, we use high resolution mass spectrometry to identify a full repertoire of post-translational modifications on histones of the marine diatom Phaeodactylum tricornutum, including eight novel modifications. We map five histone marks coupled with expression data and show that P. tricornutum displays both unique and broadly conserved chromatin features, reflecting the chimeric nature of its genome. Combinatorial analysis of histone marks and DNA methylation demonstrates the presence of an epigenetic code defining activating or repressive chromatin states. We further profile three specific histone marks under conditions of nitrate depletion and show that the histone code is dynamic and targets specific sets of genes. Conclusions: This study is the first genome-wide characterization of the histone code from a stramenopile and a marine phytoplankton. The work represents an important initial step for understanding the evolutionary history of chromatin and how epigenetic modifications affect gene expression in response to environmental cues in marine environments. © 2015 Veluchamy et al.

  16. Analysis of the Relevance of Posts in Asynchronous Discussions

    Science.gov (United States)

    Azevedo, Breno T.; Reategui, Eliseo; Behar, Patrícia A.

    2014-01-01

    This paper presents ForumMiner, a tool for the automatic analysis of students' posts in asynchronous discussions. ForumMiner uses a text mining system to extract graphs from texts that are given to students as a basis for their discussion. These graphs contain the most relevant terms found in the texts, as well as the relationships between them.…

  17. Advanced Post-Irradiation Examination Capabilities Alternatives Analysis Report

    Energy Technology Data Exchange (ETDEWEB)

    Jeff Bryan; Bill Landman; Porter Hill

    2012-12-01

    An alternatives analysis was performed for the Advanced Post-Irradiation Capabilities (APIEC) project in accordance with the U.S. Department of Energy (DOE) Order DOE O 413.3B, “Program and Project Management for the Acquisition of Capital Assets”. The Alternatives Analysis considered six major alternatives: ? No Action ? Modify Existing DOE Facilities – capabilities distributed among multiple locations ? Modify Existing DOE Facilities – capabilities consolidated at a few locations ? Construct New Facility ? Commercial Partnership ? International Partnerships Based on the alternatives analysis documented herein, it is recommended to DOE that the advanced post-irradiation examination capabilities be provided by a new facility constructed at the Materials and Fuels Complex at the Idaho National Laboratory.

  18. Post-test analysis of ROSA-III Run 704

    International Nuclear Information System (INIS)

    Purpose of the ROSA-III program are to clarify phenomena in a loss-of-coolant accident of BWR and to evaluate and improve safety analysis code by integral simulation tests. This report describes post-test analysis of Run 704. Run 704 simulated a double-ended break in the main recirculation pump suction line with the whole emergency core cooling system activation. The actual test conditions were the same as those specified except a two seconds delay in the two breaks. Post-test analysis using RELAP4J code gave a transient system pressure close to that measured. However, the phase description in the core is not expressed properly in the analysis due to the insufficient phase separation model. The calculated heater surface temperature profile is thus different from that measured, similarly to other ROSA-III post-test analysis made so far. Problems for the future are measurement of flow resistance coefficients and pump characteristics for code inputs and improvement of the phase separation model. (author)

  19. In silico analysis of chimeric espA, eae and tir fragments of Escherichia coli O157:H7 for oral immunogenic applications

    Directory of Open Access Journals (Sweden)

    Rafati Sima

    2009-12-01

    Full Text Available Abstract Background In silico techniques are highly suited for both the discovery of new and development of existing vaccines. Enterohemorrhagic Escherichia coli O157:H7 (EHEC exhibits a pattern of localized adherence to host cells, with the formation of microcolonies, and induces a specific histopathological lesion (attaching/effacing. The genes encoding the products responsible for this phenotype are clustered on a 35-kb pathogenicity island. Among these proteins, Intimin, Tir, and EspA, which are expressed by attaching-effacing genes, are responsible for the attachment to epithelial cell that leads to lesions. Results We designed synthetic genes encoding the carboxy-terminal fragment of Intimin, the middle region of Tir and the carboxy-terminal part of EspA. These multi genes were synthesized with codon optimization for a plant host and were fused together by the application of four repeats of five hydrophobic amino acids as linkers. The structure of the synthetic construct gene, its mRNA and deduced protein and their stabilities were analyzed by bioinformatic software. Furthermore, the immunogenicity of this multimeric recombinant protein consisting of three different domains was predicted. Conclusion a structural model for a chimeric gene from LEE antigenic determinants of EHEC is presented. It may define accessibility, solubility and immunogenecity.

  20. Progress in Chimeric Vector and Chimeric Gene Based Cardiovascular Gene Therapy

    Institute of Scientific and Technical Information of China (English)

    HU Chun-Song; YOON Young-sup; ISNER Jeffrey M.; LOSORDO Douglas W.

    2003-01-01

    Gene therapy for cardiovascular diseases has developed from preliminary animal experiments to clinical trials. However, vectors and target genes used currently in gene therapy are mainly focused on viral, nonviral vector and single target gene or monogene. Each vector system has a series of advantages and limitations. Chimeric vectors which combine the advantages of viral and nonviral vector,chimeric target genes which combine two or more target genes and novel gene delivery modes are being developed. In this article, we summarized the progress in chimeric vectors and chimeric genes based cardiovascular gene therapy, which including proliferative or occlusive vascular diseases such as atheroslerosis and restenosis, hypertonic vascular disease such as hypertension and cardiac diseases such as myocardium ischemia, dilated cardiomyopathy and heart failure, even heart transplantation. The development of chimeric vector, chimeric gene and their cardiovascular gene therapy is promising.

  1. Content Analysis of Tobacco-related Twitter Posts

    OpenAIRE

    Myslín, Mark; Zhu, Shu-Hong; Conway, Michael

    2013-01-01

    Objective We present results of a content analysis of tobacco-related Twitter posts (tweets), focusing on tweets referencing e-cigarettes and hookah. Introduction Vast amounts of free, real-time, localizable Twitter data offer new possibilities for public health workers to identify trends and attitudes that more traditional surveillance methods may not capture, particularly in emerging areas of public health concern where reliable statistical evidence is not readily accessible. Existing appli...

  2. Post-dryout heat transfer analysis by Lagrangian simulation

    International Nuclear Information System (INIS)

    Post-dryout heat transfer analysis model by Lagrangian simulation was developed. In the model, wall-vapor forced convective heat transfer, wall-droplet direct contact heat transfer and vapor-droplet forced convective heat transfer model were introduced. Furthermore by using Lagrangian simulation, the interaction between steam and droplet can be considered. In the sample calculation, the results of wall temperature were shown. (author)

  3. FARO base case post-test analysis by COMETA code

    International Nuclear Information System (INIS)

    The paper analyzes the COMETA (Core Melt Thermal-Hydraulic Analysis) post test calculations of FARO Test L-11, the so-called Base Case Test. The FARO Facility, located at JRC Ispra, is used to simulate the consequences of Severe Accidents in Nuclear Power Plants under a variety of conditions. The COMETA Code has a 6 equations two phase flow field and a 3 phases corium field: the jet, the droplets and the fused-debris bed. The analysis shown that the code is able to pick-up all the major phenomena occurring during the fuel-coolant interaction pre-mixing phase

  4. FARO base case post-test analysis by COMETA code

    Energy Technology Data Exchange (ETDEWEB)

    Annunziato, A.; Addabbo, C. [Joint Research Centre, Ispra (Italy)

    1995-09-01

    The paper analyzes the COMETA (Core Melt Thermal-Hydraulic Analysis) post test calculations of FARO Test L-11, the so-called Base Case Test. The FARO Facility, located at JRC Ispra, is used to simulate the consequences of Severe Accidents in Nuclear Power Plants under a variety of conditions. The COMETA Code has a 6 equations two phase flow field and a 3 phases corium field: the jet, the droplets and the fused-debris bed. The analysis shown that the code is able to pick-up all the major phenomena occurring during the fuel-coolant interaction pre-mixing phase.

  5. Prognostic Utility of Routine Chimerism Testing at 2 – 6 Months after Allogeneic Hematopoietic Cell Transplantation

    Science.gov (United States)

    Mossallam, Ghada I.; Kamel, Azza M.; Storer, Barry; Martin, Paul J.

    2009-01-01

    The utility of routine chimerism analysis as a prognostic indicator of subsequent outcomes after allogeneic hematopoietic cell transplantation (HCT) with myeloablative conditioning regimens remains controversial. To address this controversy, routine chimerism test results at 2 – 6 months after HCT with myeloablative conditioning regimens were evaluated for association with subsequent risks of chronic graft versus host disease (GVHD), non-relapse mortality (NRM), relapse and overall mortality. Only 70 (5%) of 1304 patients had <95% donor-derived cells in the marrow. Low donor chimerism in the marrow occurred predominantly among patients with low risk disease as compared to higher risk diseases and was significantly associated with a reduced risk of chronic GVHD. Among 673 patients tested, 164 (24%) had <85% donor-derived T cells in the blood. Low donor T cell chimerism occurred predominantly among patients with low risk disease as compared to higher risk diseases, among those who had conditioning with busulfan as compared to TBI, and among those with lower grades of acute GVHD. Low donor T cell chimerism in the blood was significantly associated with a reduced risk of chronic GVHD, but not with the risks of relapse, NRM or overall mortality. Routine testing of chimerism in the marrow and blood at 2 – 6 months after HCT with myeloablative conditioning regimens may be helpful in documenting engraftment in clinical trials but provides only limited prognostic information in clinical practice. PMID:19203726

  6. Generation and evaluation of a chimeric classical swine fever virus expressing a visible marker gene.

    Science.gov (United States)

    Li, Yongfeng; Wang, Xiao; Sun, Yuan; Li, Lian-Feng; Zhang, Lingkai; Li, Su; Luo, Yuzi; Qiu, Hua-Ji

    2016-03-01

    Classical swine fever virus (CSFV) is a noncytopathogenic virus, and the incorporation of an enhanced green fluorescent protein (EGFP) tag into the viral genome provides a means of direct monitoring of viral infection without immunostaining. It is well established that the 3' untranslated region (3'-UTR) of the CSFV plays an important role in viral RNA replication. Although CSFV carrying a reporter gene and chimeric CSFV have been generated and evaluated, a chimeric CSFV with a visible marker has not yet been reported. Here, we generated and evaluated a chimeric virus containing the EGFP tag and the 3'-UTR from vaccine strain HCLV (C-strain) in the genetic background of the highly virulent CSFV Shimen strain. The chimeric marker CSFV was fluorescent and had an approximately 100-fold lower viral titer, lower replication level of viral genome, and weaker fluorescence intensity than the recombinant CSFV with only the EGFP tag or the parental virus. Furthermore, the marker chimera was avirulent and displayed no viremia in inoculated pigs, which were completely protected from lethal CSFV challenge as early as 15 days post-inoculation. The chimeric marker virus was visible in vitro and attenuated in vitro and in vivo, which suggests that CSFV can be engineered to produce attenuated variants with a visible marker to facilitate in vitro studies of CSFV infection and replication and to develop of novel vaccines against CSF. PMID:26614259

  7. Regression analysis exploring teacher impact on student FCI post scores

    Science.gov (United States)

    Mahadeo, Jonathan V.; Manthey, Seth R.; Brewe, Eric

    2013-01-01

    High School Modeling Workshops are designed to improve high school physics teachers' understanding of physics and how to teach using the Modeling method. The basic assumption is that the teacher plays a critical role in their students' physics education. This study investigated teacher impacts on students' Force Concept Inventory scores, (FCI), with the hopes of identifying quantitative differences between teachers. This study examined student FCI scores from 18 teachers with at least a year of teaching high school physics. This data was then evaluated using a General Linear Model (GLM), which allowed for a regression equation to be fitted to the data. This regression equation was used to predict student post FCI scores, based on: teacher ID, student pre FCI score, gender, and representation. The results show 12 out of 18 teachers significantly impact their student post FCI scores. The GLM further revealed that of the 12 teachers only five have a positive impact on student post FCI scores. Given these differences among teachers it is our intention to extend our analysis to investigate pedagogical differences between them.

  8. Post-optimality analysis in aerospace vehicle design

    Science.gov (United States)

    Braun, Robert D.; Kroo, Ilan M.; Gage, Peter J.

    1993-01-01

    This analysis pertains to the applicability of optimal sensitivity information to aerospace vehicle design. The present analysis demonstrates that post-optimality information generated through first-order computations can be used to accurately predict file effect of constraint and parameter perturbations on the optimal solution. This assessment is based on the solution of an aircraft design problem in which the post-optimality estimates are shown to be within a few percent of the true solution over the practical range of constraint and parameter variations. Through solution of a reusable, single-stage-to-orbit, launch vehicle design problem, this optimal sensitivity information is also shown to improve the efficiency of the design process. For a hierarchically decomposed problem, this computational efficiency is realizable by estimating the main-problem objective gradient through optimal sensitivity calculations. By reducing the need for finite differentiation of a re-optimized subproblem, a significant decrease in the number of objective function evaluations required to reach the optimal solution is obtained.

  9. Chimeric molecules facilitate the degradation of androgen receptors and repress the growth of LNCaP cells

    Institute of Scientific and Technical Information of China (English)

    Yue-Qing Tang; Bang-Min Han; Xin-Quan Yao; Yan Hong; Yan Wang; Fu-Jun Zhao; Sheng-Qiang Yu; Xiao-Wen Sun; Shu-Jie Xia

    2009-01-01

    Post-translational degradation of protein plays an important role in cell life.We employed chimeric molecules (dihydrotestosterone-based proteolysis-targeting chimeric molecule [DHT-PROTAC]) to facilitate androgen receptor (AR) degradation via the ubiquitin-proteasome pathway (UPP) and to investigate the role of AR in cell proliferation and viability in androgen-sensitive prostate cancer cells.Western blot analysis and immunohistochemistry were applied to analyse AR levels in LNCaP cells after DHT-PROTAC treatment.Cell counting and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cell viability assay were used to evaluate cell proliferation and viability after AR elimination in both LNCaP and PC-3 cells.AR was tagged for elimination via the UPP by DHT-PROTAC,and this could be blocked by proteasome inhibitors.Degradation of AR depended on DHT-PROTAC concentration,and either DHT or an ALAPYIP-(arg)s peptide could compete with DHT-PROTAC.Inhibition of cell proliferation and decreased viability were observed in LNCaP cells,but not in PC-3 or 786-O cells after DHT-PROTAC treatment.These data indicate that AR elimination is facilitated via the UPP by DHT-PROTAC,and that the growth of LNCaP cells is repressed after AR degradation.

  10. Immunological tolerance and tumor rejection in embryo-aggregated chimeric mice – Lessons for tumor immunity

    International Nuclear Information System (INIS)

    Rejection of transplanted tumors by the immune system is a rare event in syngeneic hosts, and is considered to be dependent on the local interaction of defensive immune reactions and tumor tolerance mechanisms. Here, we have enlisted the aid of a unique set of embryo-aggregated lineage chimeric mice derived from C57/BL6 and FVB donors to study the interplay between local and systemic tumor immunity and tolerance in rejection of mouse B16 melanoma cells, syngeneic to the C57/BL6 donor strain. Two variants of embryo-aggregated chimeric mice with either variable or no contribution of C57-derived cells to their skin were generated by the fusion of different ratios of morula stage blastomers. Chimeric mice were analyzed for s.c. growth of B16 tumors in comparison to their respective donor strains as well as normal F1 hybrids, and the relative frequencies of cellular components of the immune system by FACS analysis of peripheral blood or lymph node cells. B16 tumors grew significantly faster in mice with full chimerism in their skin as compared to syngeneic C57 or semi-syngeneic C57 × FVB F1 hosts. In contrast, s.c. tumor growth was either absent or significantly reduced in chimeric mice lacking C57-derived cells in their skin, but tolerant to C57 tissue in other organs. Comparison of the relative frequencies of various immune cells in the periphery via FACS-analysis did not reveal any significant differences between the two types of chimeric mice with respect to their donor strains. Our data suggest a complex interplay between mechanisms of local peripheral tolerance and innate antitumor mechanisms possibly involving NK cell allorecognition as a basis for the differential growth or rejection of B16 tumors in these unique chimeric mice, which we suggest to constitute a valuable new model system for the study of immune-mediated tumor rejection

  11. Post-test analysis of PANDA test P4

    International Nuclear Information System (INIS)

    The results of a post-test analysis of the integral system test P4, which has been executed in the PANDA facility at PSI in Switzerland within the framework of Work Package 2 of the TEPSS project are presented. The post-test analysis comprises an evaluation of the PANDA test P4 and a comparison of the test results with the results of simulations using the RELAPS/MOD3.2, TRAC-BF1, and MELCOR 1.8.4 codes. The PANDA test P4 has provided data about how trapped air released from the drywell later in the transient affects PCCS performance in an adequate manner. The well-defined measurements can serve as an important database for the assessment of thermal hydraulic system analysis codes, especially for conditions that could be met in passively operated advanced reactors, i.e. low pressure and small driving forces. Based on the analysis of the test data, the test acceptance criteria have been met. The test P4 has been successfully completed and the instrument readings were with the permitted ranges. The PCCs showed a favorable and robust performance and a wide margin for decay heat removal from the containment. The PANDA P4 test demonstrated that trapped air, released from the drywell later in the transient, only temporarily and only slightly affected the performance of the passive containment cooling system. The analysis of the results of the RELAPS code showed that the overall behaviour of the test has been calculated quite well with regards to pressure, mass flow rates, and pool boil-down. This accounts both for the pre-test and the post-test simulations. However, due to the one-dimensional, stacked-volume modeling of the PANDA DW, WW, and GDCS vessels, 3D-effects such as in-vessel mixing and recirculation could not be calculated. The post-test MELCOR simulation showed an overall behaviour that is comparable to RELAPS. However, MELCOR calculated almost no air trapping in the PCC tubes that could hinder the steam condensation rate. This resulted in lower calculated

  12. Spectroscopic analysis of four post-AGB candidates

    CERN Document Server

    Molina, R E; Pereira, C B; Ferro, A Arellano; Muneer, S

    2014-01-01

    We have done a detailed abundance analysis of four unexplored candidate post- Asymptotic Giant Branch(AGB) stars IRAS 13110 - 6629, IRAS 17579 - 3121, IRAS 18321 - 1401 and IRAS 18489 - 0629 using high resolution spectra. We have constructed Spectral Energy Distributions (SED) for these objects using the existing photometric data combined with infrared (IR) fluxes. For all sample stars, the SEDs exhibit double peaked energy distribution with well separated IR peaks showing the presence of dusty circumstellar material. The CNO abundances indicate the production of N via CN cycling, but observed [C/Fe] indicates the mixing of carbon produced by He burning by third dredge up although C/O ratio remains less that 1. A moderate DG effect is clearly seen for IRAS 18489 - 0629 and IRAS 17579 - 3121 while a large scatter observed in depletion plots for IRAS 18321 - 1401 and IRAS 13110 - 6629 indicate the presence of other processes affecting the observed abundance pattern.

  13. Chimerism in health, transplantation and autoimmunity

    NARCIS (Netherlands)

    Koopmans, Marije; Kremer Hovinga, Idske Cornelia Lydia

    2009-01-01

    The term “chimerism” originates from Greek mythology and refers to the creature Chimaera, whose body was in front a lion, the back a serpent and the midsection a goat. In medicine, the term chimerism refers to an individual, organ or part consisting of tissues of diverse genetic constitution. Pregna

  14. Emoting infertility online: A qualitative analysis of men's forum posts.

    Science.gov (United States)

    Hanna, Esmée; Gough, Brendan

    2016-07-01

    Relatively little research on infertility focuses exclusively or significantly on men's experiences, particularly in relation to emotional aspects. Evidence that does exist around male infertility suggests that it is a distressing experience for men, due to stigma, threats to masculinity and the perceived need to suppress emotions, and that men and women experience infertility differently. Using thematic analysis, this article examines the online emoting of men in relation to infertility via forum posts from a men-only infertility discussion board. It was noted that men 'talked' to each other about the emotional burdens of infertility, personal coping strategies and relationships with others. Three major themes were identified following in-depth analysis: 'the emotional rollercoaster', 'the tyranny of infertility' and 'infertility paranoia'. This article then offers insights into how men experience infertility emotionally, negotiate the emotional challenges involved (especially pertaining to diagnosis, treatment outcomes and their intimate relationships) and how they share (and find value in doing so) with other men the lived experience of infertility. PMID:27246813

  15. Experience from the post-test analysis of MEGAPIE

    Energy Technology Data Exchange (ETDEWEB)

    Zanini, L., E-mail: luca.zanini@psi.ch [Paul Scherrer Institut, 5232 Villigen PSI (Switzerland); Dementjev, S.; Groeschel, F.; Leung, W.; Milenkovic, R.; Thomsen, K.; Wagner, W.; Wohlmuther, M. [Paul Scherrer Institut, 5232 Villigen PSI (Switzerland); Cheng Xu; Class, A.; Konobeyev, A. [Institut fuer Neutronenphysik und Reaktortechnik, Karlsruher Institut fuer Technologie, Postfach 3640, 76021 Karlsruhe (Germany); Agostini, P.; Meloni, P. [Ente per le nuove Tecnologie, l' Energia e l' Ambiente, Localita Brasimone, 40032 Camugnano, Bologna (Italy); David, J.-C.; Letourneau, A.; Leray, S.; Panebianco, S. [CEA - Saclay, Irfu/Service de Physique Nucleaire, F91191 Gif-sur-Yvette (France); Cachon, L.; Latge, C.; Roubin, P. [CEA, Centre de Cadarache, DEN/DTN/DIR, F-13108 Saint Paul Lez Durance (France)

    2011-08-31

    The (MEGAWatt Pilot Experiment) MEGAPIE target was successfully irradiated in 2006 at the SINQ facility of the Paul Scherrer Institut. During the irradiation a series of measurements to monitor the operation of the target, the thermal hydraulics behavior and the neutronic and nuclear aspects, has been performed. In the post-test analysis phase of the project, the data were analyzed and important information relevant to accelerator-driven systems (ADS) was gained, in particular: (i) from the operation of the target several recommendations concern the simplification of the system and the improved reliability; (ii) data from the thermal hydraulic measurements have offered the opportunity to validate the codes used in the design phase; (iii) the neutronic analysis confirm the high performance of a liquid metal target and the importance of the delayed neutron measurements in an ADS target; (iv) the nuclear measurements of the gas released gave the opportunity to validate the codes used during the design phase and provided indications for the operation. From the results in these different domains recommendations to further development of ADS and heavy liquid metal targets are discussed.

  16. Hazard analysis system of urban post-earth-quake fire based on GIS

    Institute of Scientific and Technical Information of China (English)

    李杰; 江建华; 李明浩

    2001-01-01

    The authors study the structure, functions and data organization for the hazard analysis system of urban post-earthquake fire on the platform of GIS. A general hazard analysis model of the post-earthquake fire is presented. Taking Shanghai central district as background, a system for hazard analysis of the post-earthquake fire and auxili-ary decision-against fire is developed.

  17. Single tube support post thermal analysis and test results

    International Nuclear Information System (INIS)

    Cold mass structural supports used in prototype Superconducting Super Collider (SSC) 50 mm dipole magnets built at Fermilab and Brookhaven are adaptations of the design developed during the 40 mm design program at Fermilab. The design essentially consists of two composite tubes nested within each other as a means of maximizing the thermal path length. In addition it provides an ideal way to utilize materials best suited for the temperature range over which they must operate. Filament wound S-glass is used between 300K and 80K. Filament wound graphite fiber is used between 80K and 20K and between 20K and 4.5K. An alternate design for supports which uses a single composite tube has been developed at Fermilab and continues to be refined by the industrial contractors. The advantage of the new design is cost reduction due to a significantly simpler assembly and incorporation of many common parts. This report describes the thermal analysis and testing of a single composite tube support post whose function is identical to that of the current reentrant design

  18. A post-Kyoto analysis of the Greek electric sector

    International Nuclear Information System (INIS)

    The obligations resulting from the Kyoto Protocol (KP) are implemented by many participants, e.g. Greece, by a combination of two methods: (a) application of regional measures which restrict CO2 emissions and (b) procurement of green certificates e.g. in a Climate Exchange Market (CEX). Therefore, the cost for compliance with the KP depends on the extent each method is used and also on the traded values in the CEX. The energy policy and planning to be considered are long-term items and now extension of the KP to a post-KP is discussed which reaches year 2020. In Greece, the electricity sector is with a weighted CO2 contribution of 73%, the dominantly emission sector. The paper analyzes the cost and other merits of different scenarios for the expansion of electrical power system in Greece. For different scenarios, the total cost of the electrical power system expansion is calculated as a function of the price of emission certificates. It has been shown that there is a price of 27.5 Euro /tCO2, above which specific mitigation measures may lead to cost optimum solutions. A sensitivity analysis is also presented concerning the variation of key parameters like the participation in the system expansion of new supercritical coal units, the price evolution of natural gas, the RES usage rate and the discount rates of the expansion investments. Results may be of interest for a decision on the cost optimum electrical power system expansion

  19. Finite element analysis and fracture resistance testing of a new intraradicular post

    Directory of Open Access Journals (Sweden)

    Eron Toshio Colauto Yamamoto

    2012-08-01

    Full Text Available OBJECTIVES: The objective of the present study was to evaluate a prefabricated intraradicular threaded pure titanium post, designed and developed at the São José dos Campos School of Dentistry - UNESP, Brazil. This new post was designed to minimize stresses observed with prefabricated post systems and to improve cost-benefits. MATERIAL AND METHODS: Fracture resistance testing of the post/core/root complex, fracture analysis by microscopy and stress analysis by the finite element method were used for post evaluation. The following four prefabricated metal post systems were analyzed: group 1, experimental post; group 2, modification of the experimental post; group 3, Flexi Post, and group 4, Para Post. For the analysis of fracture resistance, 40 bovine teeth were randomly assigned to the four groups (n=10 and used for the fabrication of test specimens simulating the situation in the mouth. The test specimens were subjected to compressive strength testing until fracture in an EMIC universal testing machine. After fracture of the test specimens, their roots were sectioned and analyzed by microscopy. For the finite element method, specimens of the fracture resistance test were simulated by computer modeling to determine the stress distribution pattern in the post systems studied. RESULTS: The fracture test presented the following averages and standard deviation: G1 (45.63±8.77, G2 (49.98±7.08, G3 (43.84±5.52, G4 (47.61±7.23. Stress was homogenously distributed along the body of the intraradicular post in group 1, whereas high stress concentrations in certain regions were observed in the other groups. These stress concentrations in the body of the post induced the same stress concentration in root dentin. CONCLUSIONS: The experimental post (original and modified versions presented similar fracture resistance and better results in the stress analysis when compared with the commercial post systems tested (08/2008-PA/CEP.

  20. Mutational analysis of the hepatitis C virus E1 glycoprotein in retroviral pseudoparticles and cell-culture-derived H77/JFH1 chimeric infectious virus particles

    DEFF Research Database (Denmark)

    Russell, R S; Kawaguchi, K; Meunier, J-C; Takikawa, S; Faulk, K; Bukh, J; Purcell, R H; Emerson, S U

    2009-01-01

    Cell entry by enveloped viruses is mediated by viral glycoproteins, and generally involves a short hydrophobic peptide (fusion peptide) that inserts into the cellular membrane. An internal hydrophobic domain within E1 (aa262-290) of hepatitis C virus (HCV) may function as a fusion peptide....... Retrovirus-based HCV-pseudotyped viruses (HCVpp; genotype 1a) containing Ala or Pro substitutions at conserved amino acid positions within this putative fusion peptide were generated. Mutation of conserved residues significantly reduced efficiency of HCVpp entry into Huh-7 cells. The majority of amino acid....... However, the S283P mutation had a different effect in the two systems as it did not increase production of infectious HCVcc. This comprehensive mutational analysis of the putative HCV fusion peptide provides insight into the role of E1 in its interaction with E2 and in HCV entry....

  1. reSpect: Software for Identification of High and Low Abundance Ion Species in Chimeric Tandem Mass Spectra

    Science.gov (United States)

    Shteynberg, David; Mendoza, Luis; Hoopmann, Michael R.; Sun, Zhi; Schmidt, Frank; Deutsch, Eric W.; Moritz, Robert L.

    2015-11-01

    Most shotgun proteomics data analysis workflows are based on the assumption that each fragment ion spectrum is explained by a single species of peptide ion isolated by the mass spectrometer; however, in reality mass spectrometers often isolate more than one peptide ion within the window of isolation that contribute to additional peptide fragment peaks in many spectra. We present a new tool called reSpect, implemented in the Trans-Proteomic Pipeline (TPP), which enables an iterative workflow whereby fragment ion peaks explained by a peptide ion identified in one round of sequence searching or spectral library search are attenuated based on the confidence of the identification, and then the altered spectrum is subjected to further rounds of searching. The reSpect tool is not implemented as a search engine, but rather as a post-search engine processing step where only fragment ion intensities are altered. This enables the application of any search engine combination in the iterations that follow. Thus, reSpect is compatible with all other protein sequence database search engines as well as peptide spectral library search engines that are supported by the TPP. We show that while some datasets are highly amenable to chimeric spectrum identification and lead to additional peptide identification boosts of over 30% with as many as four different peptide ions identified per spectrum, datasets with narrow precursor ion selection only benefit from such processing at the level of a few percent. We demonstrate a technique that facilitates the determination of the degree to which a dataset would benefit from chimeric spectrum analysis. The reSpect tool is free and open source, provided within the TPP and available at the TPP website.

  2. FACIAL EXPRESSION RECOGNITION WITH THE USE OF CHIMERIC FACE TECHNIQUE

    OpenAIRE

    Menshikova, Galina

    2010-01-01

    The aim of this study was to investigate holistic / feature processing for encoding face expressions employing the chimeric face technique. In the course of our experiment we tested the recognition accuracy of universal and chimeric countenance. As the study has revealed there was a considerable difference between distributions of subject responses depending on the localization of expression features (top / bottom parts of the face). For chimeric face identification accuracy substantially dec...

  3. Shear Force at the Cell-Matrix Interface: Enhanced Analysis for Microfabricated Post Array Detectors

    OpenAIRE

    Lemmon, Christopher A.; Sniadecki, Nathan J.; Ruiz, Sami Alom; Tan, John L.; Romer, Lewis H.; Chen, Christopher S.

    2005-01-01

    The interplay of mechanical forces between the extracellular environment and the cytoskeleton drives development, repair, and senescence in many tissues. Quantitative definition of these forces is a vital step in understanding cellular mechanosensing. Microfabricated post array detectors (mPADs) provide direct measurements of cell-generated forces during cell adhesion to extracellular matrix. A new approach to mPAD post labeling, volumetric imaging, and an analysis of post bending mechanics d...

  4. Post-stroke infection: A systematic review and meta-analysis

    OpenAIRE

    Dijkgraaf Marcel G; Vermeij Jan-Dirk; Nederkoorn Paul J; Westendorp Willeke F; van de Beek Diederik

    2011-01-01

    Abstract Background stroke is the main cause of disability in high-income countries, and ranks second as a cause of death worldwide. Patients with acute stroke are at risk for infections, but reported post-stroke infection rates vary considerably. We performed a systematic review and meta-analysis to estimate the pooled post-stroke infection rate and its effect on outcome. Methods MEDLINE and EMBASE were searched for studies on post-stroke infection. Cohort studies and randomized clinical tri...

  5. An analysis of three post launch evasion strategies

    OpenAIRE

    Forrest, R. N.

    1984-01-01

    The report addresses three post launch evasion strategies, velocity distribution which results in a uniform position between two concentric circles at any time is derived in an appendix. Prepared for: Strategic Systems Projects Office, Arlington, VA http://archive.org/details/analysisofthreep00forr N0003033AF31319 NA

  6. Repository post-sealing risk analysis using MACRO

    International Nuclear Information System (INIS)

    MACRO, a code to propagate probability distributions through a set of linked models, is currently under development at Lawrence Livermore Laboratory. An early version of this code, MACRO1, has been used to assess post-sealing dose to man for simple repository and site models based on actual site data

  7. Engineering Post-Graduate Programmes: A Quality and Productivity Analysis

    Science.gov (United States)

    Soares de Mello, Joao Carlos C. B.; Gomes, Eliane Goncalves; Meza, Lidia Angulo; Soares de Mello, Maria Helena C.; Soares de Mello, Adelino Jose R.

    2006-01-01

    Post-graduate courses are periodically evaluated in accordance with not always very clear criteria by CAPES (Co-ordinating Committee for Teaching Staff Improvement). These criteria try to measure, among other things, academic productivity (including quantity and quality of scientific production), number of classes given by teachers, time to…

  8. Evidence for transcript networks composed of chimeric RNAs in human cells.

    Science.gov (United States)

    Djebali, Sarah; Lagarde, Julien; Kapranov, Philipp; Lacroix, Vincent; Borel, Christelle; Mudge, Jonathan M; Howald, Cédric; Foissac, Sylvain; Ucla, Catherine; Chrast, Jacqueline; Ribeca, Paolo; Martin, David; Murray, Ryan R; Yang, Xinping; Ghamsari, Lila; Lin, Chenwei; Bell, Ian; Dumais, Erica; Drenkow, Jorg; Tress, Michael L; Gelpí, Josep Lluís; Orozco, Modesto; Valencia, Alfonso; van Berkum, Nynke L; Lajoie, Bryan R; Vidal, Marc; Stamatoyannopoulos, John; Batut, Philippe; Dobin, Alex; Harrow, Jennifer; Hubbard, Tim; Dekker, Job; Frankish, Adam; Salehi-Ashtiani, Kourosh; Reymond, Alexandre; Antonarakis, Stylianos E; Guigó, Roderic; Gingeras, Thomas R

    2012-01-01

    The classic organization of a gene structure has followed the Jacob and Monod bacterial gene model proposed more than 50 years ago. Since then, empirical determinations of the complexity of the transcriptomes found in yeast to human has blurred the definition and physical boundaries of genes. Using multiple analysis approaches we have characterized individual gene boundaries mapping on human chromosomes 21 and 22. Analyses of the locations of the 5' and 3' transcriptional termini of 492 protein coding genes revealed that for 85% of these genes the boundaries extend beyond the current annotated termini, most often connecting with exons of transcripts from other well annotated genes. The biological and evolutionary importance of these chimeric transcripts is underscored by (1) the non-random interconnections of genes involved, (2) the greater phylogenetic depth of the genes involved in many chimeric interactions, (3) the coordination of the expression of connected genes and (4) the close in vivo and three dimensional proximity of the genomic regions being transcribed and contributing to parts of the chimeric RNAs. The non-random nature of the connection of the genes involved suggest that chimeric transcripts should not be studied in isolation, but together, as an RNA network. PMID:22238572

  9. Plug-in Based Analysis Framework for LHC Post-Mortem Analysis

    CERN Document Server

    Gorbonosov, R; Zerlauth, M; Baggiolini, V

    2014-01-01

    Plug-in based software architectures [1] are extensible, enforce modularity and allow several teams to work in parallel. But they have certain technical and organizational challenges, which we discuss in this paper. We gained our experience when developing the Post-Mortem Analysis (PMA) system, which is a mission critical system for the Large Hadron Collider (LHC). We used a plugin-based architecture with a general-purpose analysis engine, for which physicists and equipment experts code plugins containing the analysis algorithms. We have over 45 analysis plugins developed by a dozen of domain experts. This paper focuses on the design challenges we faced in order to mitigate the risks of executing third-party code: assurance that even a badly written plugin doesn't perturb the work of the overall application; plugin execution control which allows to detect plugin misbehaviour and react; robust communication mechanism between plugins, diagnostics facilitation in case of plugin failure; testing of the plugins be...

  10. Design and analysis of post-marketing research.

    Science.gov (United States)

    Zhou, Xiao-Hua Andrew; Yang, Wei

    2013-07-01

    A post-marketing study is an integral part of research that helps to ensure a favorable risk-benefit profile for approved drugs used in the market. Because most of post-marketing studies use observational designs, which are liable to confounding, estimation of the causal effect of a drug versus a comparative one is very challenging. This article focuses on methodological issues of importance in designing and analyzing studies to evaluate the safety of marketed drugs, especially marketed traditional Chinese medicine (TCM) products. Advantages and limitations of the current designs and analytic methods for postmarketing studies are discussed, and recommendations are given for improving the validity of postmarketing studies in TCM products. PMID:23818199

  11. The rapid generation of chimerical genes expanding protein diversity in zebrafish

    Directory of Open Access Journals (Sweden)

    Zou Ming

    2010-11-01

    Full Text Available Abstract Background Variation of gene number among species indicates that there is a general process of new gene origination. One of the major mechanism providing raw materials for the origin of new genes is gene duplication. Retroposition, as a special type of gene duplication- the RNA-based duplication, has been found to play an important role in new gene evolution in mammals and plants, but little is known about the process in the teleostei genome. Results Here we screened the zebrafish genome for identification of retrocopies and new chimerical retrogenes and investigated their origination and evolution. We identified 652 retrocopies, of which 440 are intact retrogenes and 212 are pseudogenes. Retrocopies have long been considered evolutionary dead ends without functional significance due to the presumption that retrocopies lack the regulatory element needed for expression. However, 437 transcribed retrocopies were identified from all of the retrocopies. This discovery combined with the substitution analysis suggested that the majority of all retrocopies are subject to negative selection, indicating that most of the retrocopies may be functional retrogenes. Moreover, we found that 95 chimerical retrogenes had recruited new sequences from neighboring genomic regions that formed de novo splice sites, thus generating new intron-containing chimeric genes. Based on our analysis of 38 pairs of orthologs between Cyprinus carpio and Danio rerio, we found that the synonymous substitution rate of zebrafish genes is 4.13×10-9 substitution per silent site per year. We also found 10 chimerical retrogenes that were created in the last 10 million years, which is 7.14 times the rate of 0.14 chimerical retrogenes per million years in the primate lineage toward human and 6.25 times the rate of 0.16 chimerical genes per million years in Drosophila. This is among the most rapid rates of generation of chimerical genes, just next to the rice. Conclusion There is

  12. An E2-Substituted Chimeric Pestivirus With DIVA Vaccine Properties

    DEFF Research Database (Denmark)

    Rasmussen, Thomas Bruun; Uttenthal, Åse; Nielsen, Jens;

    An advantage of the use of chimeric pestiviruses as modified live vaccines against classical swine fever (CSF) resides in their capacity to be manipulated to achieve the characteristics desired for safe and efficacious DIVA vaccines. We have recently generated a new chimeric virus, Riems26_E2gif...

  13. An Empirical Analysis of Post-Merger Organizational Integration

    DEFF Research Database (Denmark)

    Smeets, Valerie Anne Rolande; Ierulli, Kathryn; Gibbs, Michael

    2016-01-01

    We study post-merger organizational integration using linked employer-employee data. Integration is implemented by reassigning a small number of high skilled workers, especially in R&D and management. Workforce mixing is concentrated to establishments set up after merger rather than to previously...... existing establishments. Worker turnover is high after merger, but new hiring yields stable total employment. Target employees have higher turnover and reassignment, particularly if the target firm is small relative to the acquiring firm. These findings may suggest integration is costly, but can be...

  14. Enhancement of mucosal immune responses by chimeric influenza HA/SHIV virus-like particles

    International Nuclear Information System (INIS)

    To enhance mucosal immune responses using simian/human immunodeficiency virus-like particles (SHIV VLPs), we have produced novel phenotypically mixed chimeric influenza HA/SHIV VLPs and used them to immunize C57BL/6J mice intranasally. Antibody and cytotoxic T-cell (CTL) responses as well as cytokine production in both systemic and mucosal sites were compared after immunization with SHIV VLPs or chimeric HA/SHIV VLPs. By using enzyme-linked immunosorbent assay (ELISA), the levels of serum IgG and mucosal IgA to the HIV envelope protein (Env) were found to be highest in the group immunized with chimeric HA/SHIV VLPs. Furthermore, the highest titer of serum neutralizing antibody against HIV Env was found with the group immunized with chimeric HA/SHIV VLPs. Analysis of the IgG1/IgG2a ratio indicated that a TH1-oriented immune response resulted from these VLP immunizations. HA/SHIV VLP-immunized mice also showed significantly higher CTL responses than those observed in SHIV VLP-immunized mice. Moreover, a MHC class I restricted T-cell activation ELISPOT assay showed a mixed type of TH1/TH2 cytokines in the HA/SHIV VLP-immunized mice, indicating that the chimeric VLPs can enhance both humoral and cellular immune responses to the HIV Env protein at multiple mucosal and systemic sites. The results indicate that incorporation of influenza HA into heterotypic VLPs may be highly effective for targeting vaccines to mucosal surfaces

  15. Perturbation analysis on post-buckling behavior of pile

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The nonlinear large deflection differential equation, based on the assumption that the subsoil coefficient is the 2nd root of the depth, was established by energy method. The perturbation parameter was introduced to transform the equation to a series of linear differential equations to be solved, and the deflection function according with the boundary condition was considered. Then,the nonlinear higher-order asymptotic solution of post-buckling behavior of a pile was obtained by parameter-substituting. The influencing factors such as bury-depth ratio and stiffness ratio of soil to pile, slenderness ratio on the post-buckling behavior of a pile were analyzed. The results show that the pile is more unstable when the bury-depth ratio and stiffness ratio of soil to pile increase,and although the buckling load increases with the stiffness of soil, the pile may ruin for its brittleness. Thus, in the region where buckling behavior of pile must be taken into account, the high grade concrete is supposed to be applied, and the dynamic buckling behavior of pile needs to be further studied.

  16. Post Buckling Progressive Failure Analysis of Composite Laminated Stiffened Panels

    Science.gov (United States)

    Anyfantis, Konstantinos N.; Tsouvalis, Nicholas G.

    2012-06-01

    The present work deals with the numerical prediction of the post buckling progressive and final failure response of stiffened composite panels based on structural nonlinear finite element methods. For this purpose, a progressive failure model (PFM) is developed and applied to predict the behaviour of an experimentally tested blade-stiffened panel found in the literature. Failure initiation and propagation is calculated, owing to the accumulation of the intralaminar failure modes induced in fibre reinforced composite materials. Hashin failure criteria have been employed in order to address the fiber and matrix failure modes in compression and tension. On the other hand, the Tsai-Wu failure criterion has been utilized for addressing shear failure. Failure detection is followed with the introduction of corresponding material degradation rules depending on the individual failure mechanisms. Failure initiation and failure propagation as well as the post buckling ultimate attained load have been numerically evaluated. Final failure behaviour of the simulated stiffened panel is due to sudden global failure, as concluded from comparisons between numerical and experimental results being in good agreement.

  17. Co-transformation of canola by chimeric chitinase and tlp genes towards improving resistance to Sclerotinia sclerotiorum.

    Science.gov (United States)

    Aghazadeh, Rustam; Zamani, Mohammadreza; Motallebi, Mostafa; Moradyar, Mehdi; Moghadassi Jahromi, Zahra

    2016-09-01

    Canola (Brassica napus) plants were co-transformed with two pathogenesis-related protein genes expressing a Trichoderma atroviride chitinase with a chitin-binding domain (chimeric chitinase) and a thaumatin-like protein (tlp) from Oryza sativa conferring resistance to phytopatogenic fungi by Agrobacterium-mediated transformation. The putative transgenic plants were confirmed by PCR. After measuring the specific activity of the chimeric chitinase and glucanase activity for tlp genes, transgenic plants with high specific activity were selected for southern blot analysis to confirm the copy number of the genes. In vitro assays, the antifungal activity of crude extracted protein against Sclerotinia sclerotiorum showed that the inhibition percentage in double transgenic plants was between 55 and 62, whereas the inhibition percentage in single-gene transformants (chimeric chitinase) ranged from 35 to 45 percent. Importantly, in greenhouse conditions, the double transgenic plants showed significant resistance than the single-gene transformant and wild type plants. The results in T2 generation using the intact leaf inoculation method showed that the average lesion diameters were 10, 14.7 and 29 mm for the double transformant, single-gene transformant and non-transgenic plants, respectively. Combined expression of chimeric chitinase and tlp in transgenic plants showed significantly enhanced resistance against S. sclerotiorum than the one that express single-gene transformant plants. These results suggest that the co-expression of chimeric chitinase and tlp can confer enhanced disease resistance in canola plant. PMID:27430511

  18. Reduction of porcine circovirus type 2 (PCV2 viremia by a reformulated inactivated chimeric PCV1-2 vaccine-induced humoral and cellular immunity after experimental PCV2 challenge

    Directory of Open Access Journals (Sweden)

    Seo Hwi

    2012-10-01

    Full Text Available Abstract Background The objective of the present study was to elucidate the humoral and cellular immune response mechanisms by which a reformulated inactivated chimeric PCV1-2 vaccine reduces the PCV2 viremia. Forty PCV2 seronegative 3-week-old pigs were randomly divided into the following four groups: vaccinated challenged (T01, vaccinated non-challenged (T02, non-vaccinated challenged (T03, and non-vaccinated non-challenged (T04 animals. The pigs in groups T01 and T02 were immunized with a reformulated inactivated chimeric PCV1-2 vaccine (Fostera™ PCV; Pfizer Animal Health administered as a 2.0 ml dose at 21 days of age. At 35 days of age (0 days post-challenge, the pigs in groups T01 and T03 were inoculated intranasally with 2 ml each of PCV2b. Results A reduction of PCV2 viremia coincided with the appearance of both PCV2-specific neutralizing antibodies (NA and interferon-γ-secreting cells (IFN-γ-SCs in the vaccinated animals. However, the presence of anti-PCV2 IgG antibodies did not correlate with the reduction of PCV2 viremia. Lymphocyte subset analysis indicated that the numbers of CD3+ and CD4+ cells increased in vaccinated animals but the numbers of CD4+ cells decreased transiently in non-vaccinated animals. The observation of a delayed type hypersensitivity response in only the vaccinated animals also supports a CD4+ cell-associated protective cellular immune response induced by the reformulated inactivated chimeric PCV1-2 vaccine. Conclusions The induction of PCV2-specific NA and IFN-γ-SCs, and CD4+ cells by the reformulated inactivated chimeric PCV1-2 vaccine is the important protective immune response leading to reduction of the PCV2 viremia and control of the PCV2 infection. To our knowledge this is the first demonstration of protective humoral and cellular immunity induced by the reformulated inactivated chimeric PCV1-2 vaccine and its effect on reduction of PCV2 viremia by vaccination.

  19. Regression analysis of post-CHF flow boiling data

    International Nuclear Information System (INIS)

    The successful application of statistical analysis in systematic investigations of heat transfer data for boiling water beyond the critical heat flux is described. Multiple linear regression analysis together with statistical tests of correlations and data were used in this study. Data from a number of experiments encompassing film and transition boiling in several geometries were correlated by boiling regime, by geometry, and in aggregate. Error estimates and uncertainty bounds were specified for all such correlations. (U.S.)

  20. Post Hoc Tourist Segmentation with Conjoint and Cluster Analysis

    Directory of Open Access Journals (Sweden)

    Sérgio Dominique Ferreira Lopes

    2009-01-01

    Full Text Available En el presente trabajo los autores pretenden ilustrar las ventajas del uso combinado del Análisis Conjunto y del Análisis de Conglomerados, en la segmentación del mercado turístico. Los beneficios son fácilmente entendidos, una vez que el Análisis Conjunto permite a los investigadores conocer la estructura de las preferencias de los consumidores y el Análisis de Conglomerados los agrupa en segmentos, a partir de las preferencias de éstos. Habida cuenta de la enorme complejidad y diversificación que está adquiriendo el mercado turístico en nuestros días, carece de sentido adoptar estrategias de segmentación a priori, basadas únicamente en variables clásicas de corte sociodemográfico, cuya capacidad explicativa ha demostrado ser muy limitada. En su lugar, optar por procedimientos de segmentación post hoc, donde se incluya información más elaborada, como pueden ser las preferencias de los consumidores turistas (estimadas a partir de procedimientos estadísticos avanzados como el Análisis Conjunto, se convierte en una ventaja competitiva. La segmentación basada en las preferencias permite a investigadores y gestores disponer de un conocimiento más preciso del mercado y desarrollar estrategias de Marketing adecuadas a cada uno de los segmentos de interés.

  1. The stress evaluation of root posts using the finite element analysis

    Directory of Open Access Journals (Sweden)

    João César Zielak

    2011-04-01

    Full Text Available Introduction and objective: The aim of this investigation was to evaluate through Finite Element Analysis (FEA the displacement and stress distribution of prefabricated serrated and tapered dental root posts of different compositions: carbon fiber, glass fiber and titanium. Material and methods: Through this biomechanical analysis a carbon fiber post (CF, a glass fiber post (GF and a titanium post (TI were compared according to their design (tapered [T] and serrated [S] and under a load of 250 N, at 45º, in order to simulate an occlusal load of a canine tooth. Results: FEA demonstrated the following maximum displacement (MXD in millimeters: T.CF (tapered carbon fiber post = 2.48 mm; T.GF = 1.58 mm; T.TI = 0.50 mm; S.CF = 7.66 mm; S.GF = 4.87mm. Conclusion: These results showed that the carbon fiber post demonstrated the greatest displacement, followed by the glass fiber and the titanium posts. The tapered design presented detachment values much lower than the serrated design.

  2. Post-processing tools for nonlinear fe analysis of concrete structures

    OpenAIRE

    CERVENKA, Vladimir; Pukl, Radomir; Eligehausen, Rolf

    1990-01-01

    Finite clement analysis of the cracking process in concrete structures brings new requirements for the post-processing environment. Crack direction and location are important for identification of the failure mode. The problem have been solved in the finite element program SBETA which was developed by the authors for simulation of the failure processes in reinforced concrete structures. The post-processing system creates the graphical images of crack patterns. Graphical sequences for simulati...

  3. Oxidative stress and the mechanical properties of naturally occurring chimeric collagen-containing fibers.

    OpenAIRE

    Sun, C.; E. Vaccaro; Waite, J. H.

    2001-01-01

    The byssal threads of marine mussels are a fiber-reinforced composite material. Fibers are continuous, separated by matrix, and consist of chimeric collagens that encompass within the same primary protein structure domains corresponding to collagen, polyhistidine, and either elastin or dragline spider silk. The elastic modulus (stiffness) of the proximal portion of byssal threads was measured by cyclic stress-strain analysis at 50% extension. Before measurement, the threads were conditioned b...

  4. GAME ANALYSIS OF KYOTO AND POST-KYOTO SCHEMES

    Energy Technology Data Exchange (ETDEWEB)

    Haruo Imai [Kyoto Institute of Economic Research, Kyoto University, Sakyo, Kyoto (Japan)

    2008-09-30

    Kyoto protocol, put in force in Feb. 2005, is criticized from both sides, those demanding a stricter target for GHG (green house gas) emission reduction on the one side, and those claiming for more flexible and comprehensive controls of the emission on the other side, for its modest target and narrow coverage. Even though its value could be that of a mere precedent and experimentation, Kyoto protocol includes very special experimentation to assist the world wide cooperation for a mitigation of climate change, i.e. the introduction of three mechanisms, emissions trading, joint implementation, and clean development mechanism (CDM). Together, they are called Kyoto mechanisms. Evaluation of mechanisms is one important role of microeconomics and the game theory is a major tool for it. We shall scrutinize these mechanisms from such viewpoint. A special attention is placed on CDM, as it is the novel mechanism introduced by Kyoto protocol, and gives a unique link between Annex I nations (mostly developed countries) and non-Annex I nations (mostly developing countries). Next, we examine some of the currently proposed schemes after 2013, the post Kyoto schemes. One of the chief issues is the possibility of making a comprehensive agreement including both the USA and large developing countries with rapidly increasing emission levels of GHG like China and India. Adding to these, not only the proposed schemes themselves, but the process of negotiation itself inspired several researches in cooperative game theory and in particular, coalition formation theory. We shall touch upon this issue separately, and examine how successfully they predicted the outcome leading to Kyoto, retrospectively. Finally, we end our discussion with a brief consideration over the underlining normative argument concerning these schemes.

  5. Efficient post HPLC column generation of arsanes for speciation analysis

    Czech Academy of Sciences Publication Activity Database

    Marschner, Karel; Musil, Stanislav; Rychlovský, P.; Dědina, Jiří

    2015. s. 69-70. [Colloquium Spectroscopicum Internationale /39./. 30.08.2015-03.09.2015, Figueira da Foz] R&D Projects: GA ČR GA14-23532S Grant ostatní: GA AV ČR(CZ) M200311202 Institutional support: RVO:68081715 Keywords : arsenic speciation analysis * hydride generation * HPLC Subject RIV: CB - Analytical Chemistry, Separation

  6. Finite element analysis of weakened roots restored with composite resin and posts.

    Science.gov (United States)

    Coelho, Carla Santina de Miranda; Biffi, João Carlos Gabrielli; Silva, Gisele Rodrigues da; Abrahão, Anthony; Campos, Roberto Elias; Soares, Carlos José

    2009-11-01

    Finite element analysis (FEA) was used to investigate the influence of different post systems on the stress distribution of weakened teeth under oblique-load application. A maxillary central incisor root obtained from a sound tooth was weakened by partial removal of dentin inside the root canal. Seven two-dimensional numerical models, one from the sound tooth and six from the weakened root restored with composite resin and post systems were created as follows - ST: sound tooth; CPC: cast CuAl post and core; SSP: stainless steel post + composite core; GP: fiberglass + composite core; CP: carbon fiber + composite core; ZP: zirconium dioxide post + composite core; TP: titanium post + composite core. The numerical models were considered to be restored with a leucite-reinforced all-ceramic crown and received a 45 masculine occlusal load (10 N) on the lingual surface.All the materials and structures were considered linear elastic, homogeneous, and isotropic, with the exception of fiberglass and carbon fiber posts which assumed orthotropic behavior. The numerical models were plotted and meshed with isoparametric elements, and the results were analyzed using von Mises and Sy stress criteria. When compared with the sound tooth, FEA revealed differences in stress distribution when post systems were used. Among the restored teeth, the use of CPC, SSP, ZP, and TP resulted in higher stress concentration in the post itself when compared to GP and CP. Therefore, results from the FEA images suggested that the use of non-metallic post systems could result in improved mechanical behavior for the weakened restored teeth. PMID:20019417

  7. FEBEX Project Post-mortem Analysis: Corrosion Study

    Energy Technology Data Exchange (ETDEWEB)

    Madina, V.; Azkarate, I.

    2004-07-01

    The partial dismantling of the FEBEX in situ test was carried out during de summer of 2002, following 5 years of continuous heating. The operation included the demolition of the concrete plug and the removal of the section of the test corresponding to the first heater. A large number of samples from all types of materials have been taken during the dismantling for subsequent analysis. Part of the samples collected were devoted to the analysis of the corrosion processes occurred during the first operational phase of the test. These samples comprised corrosion coupons from different metals installed for that purpose, sensors retrieved during the dismantling that were found severely corroded and bentonite in contact with those sensors. In addition, a corrosion study was performed on the heater extracted and on one section of liner surrounding it. All the analyses were carried out by the Fundacion INASMET (Spain). This report describes, in detail the studies carried out the different materials and the obtained results, as well as the drawn conclusions. (Author)

  8. Calcium-stimulated autophosphorylation site of plant chimeric calcium/calmodulin-dependent protein kinase

    Science.gov (United States)

    Sathyanarayanan, P. V.; Siems, W. F.; Jones, J. P.; Poovaiah, B. W.

    2001-01-01

    The existence of two molecular switches regulating plant chimeric Ca(2+)/calmodulin-dependent protein kinase (CCaMK), namely the C-terminal visinin-like domain acting as Ca(2+)-sensitive molecular switch and calmodulin binding domain acting as Ca(2+)-stimulated autophosphorylation-sensitive molecular switch, has been described (Sathyanarayanan, P. V., Cremo, C. R., and Poovaiah, B. W. (2000) J. Biol. Chem. 275, 30417-30422). Here we report the identification of Ca(2+)-stimulated autophosphorylation site of CCaMK by matrix-assisted laser desorption ionization time of flight-mass spectrometry. Thr(267) was confirmed as the Ca(2+)-stimulated autophosphorylation site by post-source decay experiments and by site-directed mutagenesis. The purified T267A mutant form of CCaMK did not show Ca(2+)-stimulated autophosphorylation, autophosphorylation-dependent variable calmodulin affinity, or Ca(2+)/calmodulin stimulation of kinase activity. Sequence comparison of CCaMK from monocotyledonous plant (lily) and dicotyledonous plant (tobacco) suggests that the autophosphorylation site is conserved. This is the first identification of a phosphorylation site specifically responding to activation by second messenger system (Ca(2+) messenger system) in plants. Homology modeling of the kinase and calmodulin binding domain of CCaMK with the crystal structure of calcium/calmodulin-dependent protein kinase 1 suggests that the Ca(2+)-stimulated autophosphorylation site is located on the surface of the kinase and far from the catalytic site. Analysis of Ca(2+)-stimulated autophosphorylation with increasing concentration of CCaMK indicates the possibility that the Ca(2+)-stimulated phosphorylation occurs by an intermolecular mechanism.

  9. Do chimeric sponges have improved chances of survival?

    OpenAIRE

    Maldonado, Manuel

    1998-01-01

    It has been suggested that the capacity of fusion with both kin and genetically unrelated conspecifics to form chimeras (i.e. individuals with a mixture of genetically different cells) is evolutionarily retained In several phyla because the resulting organism obtains some selective advantages over non-chimeric conspecifics. Many demosponges are known to have fusible larvae that form young chimeric sponges, but the ecological and evolutionary significance of this phenom...

  10. Image Post-Processing and Analysis. Chapter 17

    International Nuclear Information System (INIS)

    For decades, scientists have used computers to enhance and analyse medical images. At first, they developed simple computer algorithms to enhance the appearance of interesting features in images, helping humans read and interpret them better. Later, they created more advanced algorithms, where the computer would not only enhance images but also participate in facilitating understanding of their content. Segmentation algorithms were developed to detect and extract specific anatomical objects in images, such as malignant lesions in mammograms. Registration algorithms were developed to align images of different modalities and to find corresponding anatomical locations in images from different subjects. These algorithms have made computer aided detection and diagnosis, computer guided surgery and other highly complex medical technologies possible. Nowadays, the field of image processing and analysis is a complex branch of science that lies at the intersection of applied mathematics, computer science, physics, statistics and biomedical sciences. This chapter will give a general overview of the most common problems in this field and the algorithms that address them

  11. In vivo anti-tumor activity of marine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene

    Institute of Scientific and Technical Information of China (English)

    JIAN MIN YANG; MICHAEL S FRIEDMAN; MARIANNE T HUBEN; JENNIFER FULLER; QIAO LI; ALFRED E CHANG; JAMES J MULE; KEVIN T MCDONAGH

    2006-01-01

    We have confirmed efficient anti-tumor activities of the peripheral lymphocytes transduced with a p185HER2-specific chimeric T-cell receptor gene both in murine and in human in our previous studies. To further test the feasibility of chimeric T-cell receptor in a bone marrow transplantation model, we first, made two murine tumor cell lines: MT901 and MCA-205, to express human p185HER2by retroviral gene transduction. Murine bone marrow cells were retrovirally transduced to express the chimeric T-cell receptor and gene-modified bone marrow cells were transplanted into lethally irradiated mouse. Six months post transplantation, p185HER2-positive tumor cells: MT-901/HER2 or MCA-205/HER2 was subcutaneously or intravenously injected to make mouse models simulating primary breast cancer or pulmonary metastasis. The in vivo anti-tumor effects were monitored by the size of the subcutaneous tumor or counting the tumor nodules in the lungs after India ink staining. The size of the subcutaneous tumor was significantly inhibited and the number of pulmonary nodules were significantly decreased in mouse recipients transplanted with chimeric T-cell receptor modified bone marrow cells compared with the control group. Our results suggest the efficient in vivo anti-tumor activities of chimeric T-cell receptor gene modified bone marrow cells.

  12. PU.1-Silenced Dendritic Cells Induce Mixed Chimerism and Alleviate Intestinal Transplant Rejection in Rats via a Th1 to Th2 Shift

    Directory of Open Access Journals (Sweden)

    Xingwei Xu

    2016-01-01

    Full Text Available Background/Aims: Intestinal transplantation is an effective treatment for end-stage bowel failure; however, graft rejection and the toxicity associated with non-specific immunosuppression are major limitations of this procedure. Studies have shown that mixed chimerism can produce post-transplantation immune tolerance. Here, we demonstrate that in rat intestinal transplantation, PU.1-silenced dendritic cells (DCs plus bone marrow (BM cell transfusion results in mixed chimerism, and we investigate the mechanisms responsible for the effects of mixed chimerism rejection. Methods: In a model of intestinal transplantation, male Brown Norway rats were the donors, and female Lewis rats were the recipients that were randomly divided into 4 groups: control, BM, BM-imDCs and BM-PU.1. The dynamic changes in graft morphology, rejection scoring and serum concentrations of Th1/Th2-related cytokines were investigated on postoperative days 0, 7, 14, 21, and 30. Results: The BM-PU.1 group had better graft health, milder pathologic injuries, and lower rejection grades compared with the other groups. The rates of mixed chimerism were significantly highest in the BM-PU.1 group and correlated with decreases in serum IL-2 and increases in serum IL-10. Conclusion: Transfusion of PU.1-silenced DCs and BM cells induces stable mixed chimerism and has the potential to reduce pathologic injuries via a pro-Th2 shift in the Th1/Th2 balance.

  13. Bone marrow chimeric mice reveal a dual role for CD36 in Plasmodium berghei ANKA infection

    Directory of Open Access Journals (Sweden)

    Febbraio Maria

    2007-03-01

    Full Text Available Abstract Background Adhesion of Plasmodium-infected red blood cells (iRBC to different host cells, ranging from endothelial to red blood cells, is associated to malaria pathology. In vitro studies have shown the relevance of CD36 for adhesion phenotypes of Plasmodium falciparum iRBC such as sequestration, platelet mediated clumping and non-opsonic uptake of iRBC. Different adhesion phenotypes involve different host cells and are associated with different pathological outcomes of disease. Studies with different human populations with CD36 polymorphisms failed to attribute a clear role to CD36 expression in human malaria. Up to the present, no in vivo model has been available to study the relevance of different CD36 adhesion phenotypes to the pathological course of Plasmodium infection. Methods Using CD36-deficient mice and their control littermates, CD36 bone marrow chimeric mice, expressing CD36 exclusively in haematopoietic cells or in non-haematopoietic cells, were generated. Irradiated CD36-/- and wild type mice were also reconstituted with syngeneic cells to control for the effects of irradiation. The reconstituted mice were infected with Plasmodium berghei ANKA and analysed for the development of blood parasitaemia and neurological symptoms. Results All mice reconstituted with syngeneic bone marrow cells as well as chimeric mice expressing CD36 exclusively in non-haematopoietic cells died from experimental cerebral malaria between day 6 and 12 after infection. A significant proportion of chimeric mice expressing CD36 only in haematopoietic cells did not die from cerebral malaria. Conclusion The analysis of bone marrow chimeric mice reveals a dual role of CD36 in P. berghei ANKA infection. Expression of CD36 in haematopoietic cells, most likely macrophages and dendritic cells, has a beneficial effect that is masked in normal mice by adverse effects of CD36 expression in non-haematopoietic cells, most likely endothelial cells.

  14. The Impact of Guided Notes on Post-Secondary Student Achievement: A Meta-Analysis

    Science.gov (United States)

    Larwin, Karen H.; Larwin, David A.

    2013-01-01

    The common practice of using of guided notes in the post-secondary classroom is not fully appreciated or understood. In an effort to add to the existing research about this phenomenon, the current investigation expands on previously published research and one previously published meta-analysis that examined the impact of guided notes on…

  15. Campus Sustainability Governance in Canada: A Content Analysis of Post-Secondary Institutions' Sustainability Policies

    Science.gov (United States)

    Vaughter, Philip; McKenzie, Marcia; Lidstone, Lauri; Wright, Tarah

    2016-01-01

    Purpose: This paper aims to provide an overview of a content analysis of sustainability policies from Canadian post-secondary education institutions. The paper reports findings on the orientations to sustainability evident in the policies; references to other policies within the documents; and other key themes on how sustainability is engaged in…

  16. Challenges in Post Merger Integration: an analysis based on a case study from CEE

    OpenAIRE

    Vodák, Martin

    2011-01-01

    The thesis is covering an analysis of a concrete acquisition case from CEE. Based on the review of theory a conceptual framework was created and then applied to the acquisition case in order to analyze the process of post-merger integration. As a result, recommendations were created for similar future transactions.

  17. An Analysis of Foster Care Placement History and Post-Secondary Graduation Rates

    Science.gov (United States)

    Day, Angelique; Dworsky, Amy; Feng, Wenning

    2013-01-01

    Prior research has document significant disparities in post-secondary educational attainment between young adults who had been in foster care and their peers in the general population. This study uses survival analysis to compare the four-year college graduation rate of students who had been in foster care to the graduation rate of first…

  18. No-reference analysis of decoded MPEG images for PSNR estimation and post-processing

    DEFF Research Database (Denmark)

    Forchhammer, Søren; Li, Huiying; Andersen, Jakob Dahl

    2011-01-01

    We propose no-reference analysis and processing of DCT (Discrete Cosine Transform) coded images based on estimation of selected MPEG parameters from the decoded video. The goal is to assess MPEG video quality and perform post-processing without access to neither the original stream nor the code...

  19. Analysis of anionic post-blast residues of low explosives from soil samples of forensic interest

    International Nuclear Information System (INIS)

    The growing threats and terrorist activities in recent years have urged the need for rapid and accurate forensic investigation on post-blast samples. The analysis of explosives and their degradation products in soils are important to enable forensic scientist to identify the explosives used in the bombing and establish possible links to their likely origin. Anions of interest for post-blast identification of low explosives were detected and identified using ion chromatography (IC). IC separations of five anions (Cl-, NO2-, NO3-, SO42-, SCN-) employed a Metrosep Anion Dual 2 column with carbonate eluent. The anions were separated within 17 minutes. Sampling of post blast residues was carried out in Rompin, Pahang. The post-blast explosive residues were extracted from soil samples collected at the seat of three simulated explosion points. The homemade explosives comprised of black powder of various amounts (100 g, 150 g and 200 g) packed in small plastic sauce bottles. In black powder standard, three anions (Cl-, NO3-, SO42-) were identified. However, low amounts of nitrite (NO2-) were found present in post-blast soil samples. The amounts of anions were generally found to be decreased with decreasing amount of black powder explosive used. The anions analysis was indicative that nitrates were being used as one of the black powder explosive ingredients. (author)

  20. ProteomeScout: a repository and analysis resource for post-translational modifications and proteins

    OpenAIRE

    Matlock, Matthew K.; Holehouse, Alex S.; Kristen M Naegle

    2014-01-01

    ProteomeScout (https://proteomescout.wustl.edu) is a resource for the study of proteins and their post-translational modifications (PTMs) consisting of a database of PTMs, a repository for experimental data, an analysis suite for PTM experiments, and a tool for visualizing the relationships between complex protein annotations. The PTM database is a compendium of public PTM data, coupled with user-uploaded experimental data. ProteomeScout provides analysis tools for experimental datasets, incl...

  1. Analysis of the growth of concomitant nitride layers produced by a post-discharge assisted process

    International Nuclear Information System (INIS)

    In the present work, the growth of concomitant nitride layers during a post-discharge process is studied. The analysis takes into account the similarities and differences between nitriding post-discharge processes and other nitriding processes, employing a mathematical simulation of nitrogen diffusion. The considered differences are related to the thermodynamic standard states, the nitrogen concentration on the surface and the sputtering of the surface (this one for plasma processes). Nitrogen diffusion and layer formation are described from the beginning of the process by means of a mathematical model

  2. Analysis of binding domain and function of chimeric opioid receptors to ohmefentanyl stereoisomers1%嵌合μ/κ阿片受体与羟甲芬太尼异构体结合部位和功能分析

    Institute of Scientific and Technical Information of China (English)

    周德和; 池志强; 冯亚平; 谌立伟; 徐学军

    2001-01-01

    AIM: To investigate specific domains in μ opioid receptors that accounted for selective binding of three stereoisomers of ohmefentanyl (Ohm9204, Ohm9202, and Ohm9203) and study the function of chimera Ⅱ. METHODS: Rat μ and κ opioid receptors (RMOR, RKOR) and four μ/κ chimeric receptors (chimeras) Ⅰ , Ⅱ, Ⅲ, and Ⅳ were transiently expressed in COS-1 cells. The binding ability and binding domain of receptor to ligands were determined by radioactive ligand and receptor binding experiments. Through measuring cellular cAMP levels, we studied the function of chimera Ⅱ in mediating signal transduction. RESULTS: Binding affinities of four chimeric receptors were similar to wild type opioid receptors (RMOR and RKOR). The binding affinities of Ohm9204 and Ohm9202 to chimera Ⅱ were similar to that of RMOR. The binding affinities of Ohm9203 to all six receptors were low. U50488 possessed high binding affinity to chimera Ⅰ, however dynorphie A( 1 -9) had some binding affinity to chimera Ⅱ that was similar to RKOR, which indicated the domains of RKOR accounting for selectively binding to peptide ligand dynorphie A(1- 9) and nonpeptide ligand U50488 were different. The efficacy of Ohm9204 and Ohm9203 on inhibiting forskolin-stimulated cAMP accumulation in cells transfected with chimera Ⅱ was similar to that in cells transfected with RMOR. CONCLUSION: Replacing 194- 268 residues of RMOR with 185 - 262 residues of RKOR does not influence the ability of μ opioid receptor to bind Ohm9204 and Ohm9202 and the receptor mediated inhibition of cellular cAMP level.%目的:研究嵌合μ/κ阿片受体与羟甲芬太尼异构体 的结合部位及chimeraⅡ的功能.方法:在COS-1 细胞中瞬时表达大鼠μ、κ阿片受体(RMOR、 RKOR)以及四种嵌合μ/κ阿片受体chimera Ⅰ、Ⅱ、 Ⅲ、Ⅳ.用放射性受体结合实验进行受体活性的测 定和结合区域的分析.通过测定胞内cAMP的水 平,研究了chimeraⅡ介导

  3. Quantitative proteomic analysis of post-translational modifications of human histones

    DEFF Research Database (Denmark)

    Beck, Hans Christian; Nielsen, Eva C; Matthiesen, Rune;

    2006-01-01

    quantitative proteomics of histones from HDACi-treated cells were consistent with Western blot analysis of histone acetylation, cytotoxicity, and dose-dependent expression profiles of p21 and cyclin A2. This demonstrates that mass spectrometry-based quantitative proteomic analysis of post-translational......Histone proteins are subject to a range of post-transcriptional modifications in living cells. The combinatorial nature of these modifications constitutes the "histone code" that dictates chromatin structure and function during development, growth, differentiation, and homeostasis of cells...... software for qualitative and quantitative proteomic analysis of histones extracted from human small cell lung cancer cells. A total of 32 acetylations, methylations, and ubiquitinations were located in the human histones H2A, H2B, H3, and H4, including seven novel modifications. An LC-MSMS-based method was...

  4. Post-buckling analysis of composite beams: A simple intuitive formulation

    Indian Academy of Sciences (India)

    Jagadish Babu Gunda; G Venkateswara Rao

    2013-06-01

    Post-buckling analysis of composite beams with axially immovable ends is investigated using an Intuitive formulation. Intuitive formulation uses two parameters namely critical buckling load and axial stretching force developed in the post-buckled domain of composite beam. Geometric nonlinearity of von-Karman type is taken into consideration which accounts for membrane stretching action of the beam. Axial stretching force developed in post-buckled domain of composite beam is evaluated by using an axial governing equation and is expressed either in terms of lateral displacement function as an integrated value, or as a function of both axial and lateral displacement functions at any discrete location of the beam. The available expressions of critical buckling load and derived expressions of axial stretching force developed in the beam are used for obtaining an approximate closed-form expressions for the post-buckling loads of various beam boundary conditions. Numerical accuracy of the proposed analytical closed-form expressions obtained from the intuitive formulation are compared to the available finite element solutions for symmetric and asymmetric lay-up schemes of laminated composite beam. Effect of central amplitude ratio and lay-up orientation on post-buckling load variation is briefly discussed for various beam boundary conditions considered in this study.

  5. Chimeric autologous/allogeneic constructs for skin regeneration.

    Science.gov (United States)

    Rasmussen, Cathy Ann; Tam, Joshua; Steiglitz, Barry M; Bauer, Rebecca L; Peters, Noel R; Wang, Ying; Anderson, R Rox; Allen-Hoffmann, B Lynn

    2014-08-01

    The ideal treatment for severe cutaneous injuries would eliminate the need for autografts and promote fully functional, aesthetically pleasing autologous skin regeneration. NIKS progenitor cell-based skin tissues have been developed to promote healing by providing barrier function and delivering wound healing factors. Independently, a device has recently been created to "copy" skin by harvesting full-thickness microscopic tissue columns (MTCs) in lieu of autografts traditionally harvested as sheets. We evaluated the feasibility of combining these two technologies by embedding MTCs in NIKS-based skin tissues to generate chimeric autologous/allogeneic constructs. Chimeric constructs have the potential to provide immediate wound coverage, eliminate painful donor site wounds, and promote restoration of a pigmented skin tissue possessing hair follicles, sweat glands, and sebaceous glands. After MTC insertion, chimeric constructs and controls were reintroduced into air-interface culture and maintained in vitro for several weeks. Tissue viability, proliferative capacity, and morphology were evaluated after long-term culture. Our results confirmed successful MTC insertion and integration, and demonstrated the feasibility of generating chimeric autologous/allogeneic constructs that preserved the viability, proliferative capacity, and structure of autologous pigmented skin. These feasibility studies established the proof-of-principle necessary to further develop chimeric autologous/allogeneic constructs for the treatment of complex skin defects. PMID:25102552

  6. A fast and simple approach for the simultaneous detection of hematopoietic chimerism, NPM1, and FLT3-ITD mutations after allogeneic stem cell transplantation.

    Science.gov (United States)

    Waterhouse, Miguel; Bertz, Hartmut; Finke, Juergen

    2014-02-01

    Hematopoietic chimerism can be used as a tool for patient management after allogeneic hematopoietic stem cell transplantation (HSCT). An increase in the proportion of recipient cells after transplantation is strongly associated with relapse in chronic myeloid leukemia. However, in acute myeloid leukemia (AML) the significance of increasing mixed chimerism (MC) as a predictive marker for relapse is less clear. Several mutations frequently found in AML have been employed for minimal residual disease detection and relapse prediction. Therefore, a combined analysis of hematopoietic chimerism and of the molecular aberrations found in AML could be used to improve MC characterization. We developed a multiplex PCR for use in the simultaneous detection of hematopoietic chimerism and mutations in nucleophosmin (NPM1) and fms-like tyrosine kinase-3 internal tandem duplication (FLT3-ITD). A total of 303 samples from 20 AML patients were analyzed after HSCT. The microsatellite markers used for hematopoietic chimerism detection were D1S80, D7S1517, D4S2366, THO1, and SE33. A total of 149 samples from 18 patients showed MC with a mean detection time of 9.7 months. From the 18 patients with MC, in 6 of the patients, no FLT3-ITD or NPM1 mutation was found at any time point tested, and these patients remained in complete hematological remission. In 12 patients with MC, FLT3-ITD and NPM1 mutations were found, and these patients showed signs of hematological relapse. Our combined analysis of NPM1/FLT3-ITD mutations and hematopoietic chimerism improved the characterization of patients with MC after HSCT. The present approach may be further expanded by combining additional mutations found in AML with hematopoietic chimerism detection. PMID:23907410

  7. The construction of chimeric T-Cell receptor with spacer base of modeling study of VHH and MUC1 interaction.

    Science.gov (United States)

    Pirooznia, Nazanin; Hasannia, Sadegh; Taghdir, Majid; Rahbarizadeh, Fatemeh; Eskandani, Morteza

    2011-01-01

    Adaptive cell immunotherapy with the use of chimeric receptors leads to the best and most specific response against tumors. Chimeric receptors consist of a signaling fragment, extracellular spacer, costimulating domain, and an antibody. Antibodies cause immunogenicity; therefore, VHH is a good replacement for ScFv in chimeric receptors. Since peptide sequences have an influence on chimeric receptors, the effect of peptide domains on each other's conformation were investigated. CD3Zeta, CD28, VHH and CD8α, and FcgIIα are used as signaling moieties, costimulating domain, antibody, and spacers, respectively. To investigate the influence of the ligation of spacers on the conformational structure of VHH, models of VHH were constructed. Molecular dynamics simulation was run to study the influence of the presence of spacers on the conformational changes in the binding sites of VHH. Root mean square deviation and root mean square fluctuation of critical segments in the binding site showed no noticeable differences with those in the native VHH. Results from molecular docking revealed that the presence of spacer FcgIIα causes an increasing effect on VHH with MUC1 interaction. Each of the constructs was transformed into the Jurkat E6.1. Expression analysis and evaluation of their functions were examined. The results showed good expression and function. PMID:21869862

  8. The Construction of Chimeric T-Cell Receptor with Spacer Base of Modeling Study of VHH and MUC1 Interaction

    Directory of Open Access Journals (Sweden)

    Nazanin Pirooznia

    2011-01-01

    Full Text Available Adaptive cell immunotherapy with the use of chimeric receptors leads to the best and most specific response against tumors. Chimeric receptors consist of a signaling fragment, extracellular spacer, costimulating domain, and an antibody. Antibodies cause immunogenicity; therefore, VHH is a good replacement for ScFv in chimeric receptors. Since peptide sequences have an influence on chimeric receptors, the effect of peptide domains on each other's conformation were investigated. CD3Zeta, CD28, VHH and CD8α, and FcgIIα are used as signaling moieties, costimulating domain, antibody, and spacers, respectively. To investigate the influence of the ligation of spacers on the conformational structure of VHH, models of VHH were constructed. Molecular dynamics simulation was run to study the influence of the presence of spacers on the conformational changes in the binding sites of VHH. Root mean square deviation and root mean square fluctuation of critical segments in the binding site showed no noticeable differences with those in the native VHH. Results from molecular docking revealed that the presence of spacer FcgIIα causes an increasing effect on VHH with MUC1 interaction. Each of the constructs was transformed into the Jurkat E6.1. Expression analysis and evaluation of their functions were examined. The results showed good expression and function.

  9. Cloning, expression, and purification of a highly immunogenic recombinant gonadotropin-releasing hormone (GnRH) chimeric peptide.

    Science.gov (United States)

    Xu, Jinshu; Zhu, Zheng; Duan, Peng; Li, Wenjia; Zhang, Yin; Wu, Jie; Hu, Zhuoyi; Roque, Rouel S; Liu, Jingjing

    2006-12-01

    To design an anti-gonadotropin-releasing hormone (GnRH) vaccine capable of eliciting strong immunogenicity, a gene fragment encoding a chimeric peptide was constructed using polymerase chain reaction and ligated into a novel expression vector for recombinant expression in a T7 RNA polymerase-based expression system. The chimeric peptide called GnRH3-hinge-MVP contained three linear repeats of GnRH (GnRH3), a fragment of the human IgG1 hinge region, and a T-cell epitope of measles virus protein (MVP). The expression plasmid contained the GnRH3-hinge-MVP construct ligated to its fusion partner (AnsB-C) via an unique acid labile Asp-Pro linker. The recombinant fusion protein was expressed in an inclusion body in Escherichia coli under IPTG or lactose induction and the target peptide was easily purified using washing of urea and ethanol precipitation. The target chimeric peptide was isolated from the fusion partner following acid hydrolysis and purified using DEAE-Sephacel chromatography. The purified GnRH3-hinge-MVP was determined to be highly homogeneous by IEF analysis and the N-terminal sequencing. Further, immunization of female mice with the recombinant chimeric peptide resulted in generation of high-titer antibodies specific for GnRH. The results showed that GnRH3-hinge-MVP could be considered as a candidate anti-GnRH vaccine. PMID:17064933

  10. Developmental competence of porcine chimeric embryos produced by aggregation

    DEFF Research Database (Denmark)

    Li, Juan; Jakobsen, Jannik E.; Xiong, Qiang;

    2015-01-01

    either by parthenogenetic activation (PA) or handmade cloning (HMC). Results showed that the developmental competence of chimeric embryos, evaluated based on their blastocyst rate and total cell number per blastocyst, was increased when two whole 2-cell stage embryos (PA or HMC) were aggregated. In...... comparison, when two blastomeres were aggregated, the developmental competence of the chimeric embryos decreased if the blastomeres were either from PA or from HMC embryos, but not if they were from different sources, i.e. one PA and one HMC blastomere. To evaluate the cell contribution in embryo formation......The purpose of our study was to compare the developmental competence and blastomere allocation of porcine chimeric embryos formed by micro-well aggregation. Chimeras were created by aggregating either two blastomeres originating from 2-cell embryos or two whole embryos, where embryos were produced...

  11. Teeth restored using fiber-reinforced posts: in vitro fracture tests and finite element analysis.

    Science.gov (United States)

    Schmitter, M; Rammelsberg, P; Lenz, J; Scheuber, S; Schweizerhof, K; Rues, S

    2010-09-01

    In dentistry the restoration of decayed teeth is challenging and makes great demands on both the dentist and the materials. Hence, fiber-reinforced posts have been introduced. The effects of different variables on the ultimate load on teeth restored using fiber-reinforced posts is controversial, maybe because the results are mostly based on non-standardized in vitro tests and, therefore, give inhomogeneous results. This study combines the advantages of in vitro tests and finite element analysis (FEA) to clarify the effects of ferrule height, post length and cementation technique used for restoration. Sixty-four single rooted premolars were decoronated (ferrule height 1 or 2 mm), endodontically treated and restored using fiber posts (length 2 or 7 mm), composite fillings and metal crowns (resin bonded or cemented). After thermocycling and chewing simulation the samples were loaded until fracture, recording first damage events. Using UNIANOVA to analyze recorded fracture loads, ferrule height and cementation technique were found to be significant, i.e. increased ferrule height and resin bonding of the crown resulted in higher fracture loads. Post length had no significant effect. All conventionally cemented crowns with a 1-mm ferrule height failed during artificial ageing, in contrast to resin-bonded crowns (75% survival rate). FEA confirmed these results and provided information about stress and force distribution within the restoration. Based on the findings of in vitro tests and computations we concluded that crowns, especially those with a small ferrule height, should be resin bonded. Finally, centrally positioned fiber-reinforced posts did not contribute to load transfer as long as the bond between the tooth and composite core was intact. PMID:20227533

  12. Meta-analysis: Nitroglycerin for prevention of post-ERCP pancreatitis

    DEFF Research Database (Denmark)

    Bang, Ulrich Christian; Nøjgaard, C; Andersen, P K;

    2009-01-01

    BACKGROUND: Acute pancreatitis after ERCP is a severe side effect. AIM: To evaluate the preventive effect of nitroglycerin on post-ERCP pancreatitis by a meta-analysis of randomized clinical studies. METHODS: We searched on Pubmed, Embase, Cochrane Library and all abstracts presented at Digestive...... Disease Week and United European Gastrointestinal Week from 2004 to 2008. We used the MeSH terms 'pancreatitis' together (AND) with the terms: 'glyceryl trinitrate', 'glyceryl dinitrate', 'isosorbide dinitrate' or 'nitroglycerin'. RESULTS: Five clinical studies evaluating the incidence of post......-ERCP pancreatitis after administration of nitroglycerin were identified. Meta-analysis including all five studies showed a relative risk (RR) of 0.61 (95% CI; 0.44, 0.86) with the number needed to treat (NNT) of 26 (95% CI: 16, 82). Three studies evaluated nitroglycerin administered by a dermal patch reaching...

  13. Level of literacy and dementia: A secondary post-hoc analysis from North-West India

    Directory of Open Access Journals (Sweden)

    Sunil Kumar Raina

    2014-01-01

    Full Text Available Introduction: A relation between literacy and dementia has been studied in past and an association has been documented. This is in spite of some studies pointing to the contrary. The current study was aimed at investigating the influence of level of literacy on dementia in a sample stratified by geography (Migrant, Urban, Rural and Tribal areas of sub-Himalayan state of Himachal Pradesh, India. Materials and Methods: The study was based on post-hoc analysis of data obtained from a study conducted on elderly population (60 years and above from selected geographical areas (Migrant, Urban, Rural and Tribal of Himachal Pradesh state in North-west India. Results: Analysis of variance revealed an effect of education on cognitive scores [F = 2.823, P =0.01], however, post-hoc Tukey′s HSD test did not reveal any significant pairwise comparisons. Discussion: The possibility that education effects dementia needs further evaluation, more so in Indian context.

  14. Uncertainty characterization and quantification in air pollution models. Application to the CHIMERE model

    Science.gov (United States)

    Debry, Edouard; Mallet, Vivien; Garaud, Damien; Malherbe, Laure; Bessagnet, Bertrand; Rouïl, Laurence

    2010-05-01

    probability density function (PDF) is associated with an input parameter, according to its assumed uncertainty. Then the combined PDFs are propagated into the model, by means of several simulations with randomly perturbed input parameters. One may then obtain an approximation of the PDF of modeled concentrations, provided the Monte Carlo process has reasonably converged. The uncertainty analysis with CHIMERE has been led with a Monte Carlo method on the French domain and on two periods : 13 days during January 2009, with a focus on particles, and 28 days during August 2009, with a focus on ozone. The results show that for the summer period and 500 simulations, the time and space averaged standard deviation for ozone is 16 µg/m3, to be compared with an averaged concentration of 89 µg/m3. It is noteworthy that the space averaged standard deviation for ozone is relatively constant over time (the standard deviation of the timeseries itself is 1.6 µg/m3). The space variation of the ozone standard deviation seems to indicate that emissions have a significant impact, followed by western boundary conditions. Monte Carlo simulations are then post-processed by both ensemble [4] and Bayesian [5] methods in order to assess the quality of the uncertainty estimation. (1) Rao, K.S. Uncertainty Analysis in Atmospheric Dispersion Modeling, Pure and Applied Geophysics, 2005, 162, 1893-1917. (2) Beekmann, M. and Derognat, C. Monte Carlo uncertainty analysis of a regional-scale transport chemistry model constrained by measurements from the Atmospheric Pollution Over the Paris Area (ESQUIF) campaign, Journal of Geophysical Research, 2003, 108, 8559-8576. (3) Hanna, S.R. and Lu, Z. and Frey, H.C. and Wheeler, N. and Vukovich, J. and Arunachalam, S. and Fernau, M. and Hansen, D.A. Uncertainties in predicted ozone concentrations due to input uncertainties for the UAM-V photochemical grid model applied to the July 1995 OTAG domain, Atmospheric Environment, 2001, 35, 891-903. (4) Mallet, V., and B

  15. Ethical issues in the documentary data analysis of Internet posts and archives.

    OpenAIRE

    Sixsmith, Judith; Murray, Craig

    2001-01-01

    The documentary analysis of email posts and archives for qualitative research has been outlined elsewhere (Murray & Sixsmith, 1998a; Murray and Sixsmith, in press). Although there is an increase in the number of studies being conducted on listserv and newsgroup material in health research, this has not always been accompanied by a careful, in-depth consideration of the concomitant ethical issues. Therefore, this article outlines the ethical considerations surrounding this form of research, in...

  16. Analysis of Entry Modes and Competitive Strategies for Foreign Banks in Post-WTO China

    OpenAIRE

    Huang, Yuwei

    2006-01-01

    This dissertation aims to study and analyze the entry modes and competitive strategies for foreign banks in post-WTO China. Firstly China's banking system and China's WTO commitments to open up its banking sectors are generally reviewed. Then use the PEST and SWOT analysis methods to examine the external and internal factors which have influences on foreign banks' strategies. Combined the theories with the practical situations of the chosen entry modes and competitive strategies by foreign ba...

  17. Hydraulic analysis of river training cross-vanes as part of post-restoration monitoring

    OpenAIRE

    T. A. Endreny; Soulman, M. M.

    2011-01-01

    River restoration design methods are incrementally improved by studying and learning from monitoring data in previous projects. In this paper we report post-restoration monitoring data and simulation analysis for a Natural Channel Design (NCD) restoration project along 1600 m of the Batavia Kill (14 km2 watershed) in the Catskill Mountains, NY. The restoration project was completed in 2002 with goals to reduce bank erosion and determine the efficacy of NCD approaches fo...

  18. KINETIC MODELLING AND PERFORMANCE ANALYSIS OF THE FOUR-POST-FRAME LIFTINGMECHANICAL SYSTEM

    Institute of Scientific and Technical Information of China (English)

    高建华; 杨汝清; 胡洪国

    2001-01-01

    The kinetic model of the four-post-frame lifting mechanical system was established. The stiffness and damping matrices of differential equations of motion were obtained by using Lagrange's equations. And the dynamic characteristics of system were analyzed by modal analysis method. Based upon this, the modifications of structural parameters which can improve dynamic performance were discussed. The low-level high-speed palletizer MDJ1200L was taken as a real case in the paper.

  19. Business and training needs analysis in post conflict and developing countries : a guide

    OpenAIRE

    Stephenson, M.H.

    2004-01-01

    The British Geological Survey (BGS) has a long history of capacity building in geological surveys and energy/mines ministries and departments in the developing world and post conflict situations. In recent research involving business and training needs analysis (BTNA), the relationships between such public sector bodies, their stakeholders, and large aid donors, has been studied in detail, particularly in countries of central Asia, southeast Asia and the Pacific (Stephenson et ...

  20. In Silico Analysis of Correlations between Protein Disorder and Post-Translational Modifications in Algae

    OpenAIRE

    Atsushi Kurotani; Tetsuya Sakurai

    2015-01-01

    Recent proteome analyses have reported that intrinsically disordered regions (IDRs) of proteins play important roles in biological processes. In higher plants whose genomes have been sequenced, the correlation between IDRs and post-translational modifications (PTMs) has been reported. The genomes of various eukaryotic algae as common ancestors of plants have also been sequenced. However, no analysis of the relationship to protein properties such as structure and PTMs in algae has been reporte...

  1. Post-liquefaction soil-structure interaction for buried structures: Sensitivity analysis studies

    International Nuclear Information System (INIS)

    The post liquefaction behavior of buried conduits is analyzed and sensitivity analysis is conducted to investigate the damage potential of the forces induced in the buried lifelines following seismically induced liquefaction of the surrounding soil. Various lifeline configurations and loading conditions are considered. The loading conditions considered are: buoyancy forces and permanent ground displacements parallel to the lifeline axis. Pertinent parameters for the soil-lifeline interaction following liquefaction are identified. (author)

  2. Contour Cluster Shape Analysis for Building Damage Detection from Post-earthquake Airborne LiDAR

    OpenAIRE

    2015-01-01

    Detection of the damaged building is the obligatory step prior to evaluate earthquake casualty and economic losses. It's very difficult to detect damaged buildings accurately based on the assumption that intact roofs appear in laser data as large planar segments whereas collapsed roofs are characterized by many small segments. This paper presents a contour cluster shape similarity analysis algorithm for reliable building damage detection from the post-earthquake airborne LiDAR point cloud. Fi...

  3. 以轮状病毒VP6为载体的RV/HBV嵌合蛋白的构建及抗原性研究%Construction of a rotavirus/hepatitis B virus plasmid and the immunogenic analysis of the expressed chimeric proteins

    Institute of Scientific and Technical Information of China (English)

    袁静; 吴绪伟; 郑红梅; 曹向红; 潘小霞; 滕玉梅; 李琦涵; 陈元鼎

    2012-01-01

    Objective To construct a pETP6v carrier vector that expressed rotavirus (RV) VP6 protein and insert a gene fragment encoding the epitope of hepatitis B virus (HBV) S protein that expressed a RV/HBV chimeric protein, and to study the immunogenicity of this chimeric protein and assess its potential as a combined vaccine.Methods Using gene cloning and recombinant techniques,the gene of rotavirus VP6,the group (subgroup) of antigenic protein of the virus,was inserted into plasmid pETL to construct a carrier vector named pETP6v; then,a DNA fragment from HBV S gene that encodes the highly conserved epitope consisting of 21 amino acids (TTPAQGNSMFPSCCCSKPTDG) was inserted into the carrier vector pETP6v.The expression of this rotavirus VP6/HBV S epitope chimeric protein was analyzed in E.coli and further evaluated by ELISA and Western blot.Results A RV/HBV expression plasmid (pETP6/Hs) that carries the HBV S determinant was constructed and the RV/HBV chimeric protein expressed at very high levels in E.coli (about 36.2% of the total bacterial proteins) and the purity of the recombinant protein reached above 90%.The purified chemeric protein could react with anti-HBs serum from HBV-infected individuals and serum from guinea pigs immunized with rotavirus VP6 protein by ELISA and/or Western blot analysis.Conclusions The recombinant expression plasmid pETP6/Hs can be constructed to express chimeric proteins such as RV/HBV with high immunogenicity and reactivity and may be used in the development of RV/HBV and RV/other virus combined vaccines.%目的 构建轮状病毒(rotavirus,RV) VP6载体及携带乙型肝炎病毒(hepatitis B virus,HBV)外源抗原表位的嵌合质粒,进一步探索研发携带HBV抗原表位的重组嵌合疫苗的技术.方法 运用基因克隆和重组技术,构建轮状病毒VP6载体,同时将HBV编码中和抗原表位21个氨基酸的基因序列构建到VP6载体上相应酶切位点,在大肠杆菌中表达嵌合蛋白,并进行相关

  4. PRODUCTION IN PICHIA PASTORIS AND CHARACTERIZATION OF GENETIC ENGINEERED CHIMERIC HBV/HEV VIRUS-LIKE PARTICLES

    Institute of Scientific and Technical Information of China (English)

    Hong-zhao Li; Hong-ying Gang; Qiang-ming Sun; Xiao Liu; Yan-bing Ma; Mao-sheng Sun; Chang-bai Dai

    2004-01-01

    Objective To investigate the presentation of a neutralization epitope-containing peptide antigen of hepatitis E virus (HEV)on chimeric virus-like particles (VLPs) of hepatitis B surface antigen (HBsAg).Methods The gene fragment corresponding to amino acids (aa) 551-607 (HEnAg) of HEV capsid protein, which contains the only neutralization epitope identified to date, was fused via a synthetic glycine linker in frame with the gene of HBsAg.The resulted fusion gene was then integrated through transformation into the genome of Pichiapastoris under the control of a methanol-induced alcohol oxidase 1 (A OX 1) promoter and expressed intracellularly. The expression products in the soluble cell extracts were characterized by Western blot, ELISA, CsCl density gradient analysis, and electron microscopic visualization.Results The novel fusion protein incorporating HBsAg and the neutralization epitope-containing HEnAg was expressed successfully in Pichiapastoris with an expected molecular weight of approximately 32 kD. It was found to possess the ability to assemble into chimeric HBV/HEV VLPs with immunological, physical and morphological characteristics akin to HBsAg particles. Not only did the chimeric VLPs show high activity levels in a HBsAg particle-specific ELISA but they were also strongly immunoreactive with hepatitis E (HE) positive human serum in a HEV specific ELISA, indicating that HEnAg peptide fragments were exposed on VLP surfaces and would be expected to be readily accessible by cells and molecules of the immune system. Similarity between chimeric VLPs to highly immunogenic HBsAg particles may confer good immunogenicity on surface-displayed HEnAg.Conclusion The chimeric HBV/HEV VLPs produced in this study may have potential to be a recombinant HBV/HEV bivalent vaccine candidate.

  5. Optimisation of the posterior stabilised tibial post for greater femoral rollback after total knee arthroplasty—a finite element analysis

    OpenAIRE

    Chandran, Nagarajan; Amirouche, Farid; Gonzalez, Mark H.; Hilton, Kevin M.; Barmada, Riad; Goldstein, Wayne

    2008-01-01

    Femoral rollback after total knee arthroplasty (TKA) is necessary for flexion beyond 90–100°. Femoral rollback in posterior cruciate substituting TKA occurs as a result of the interaction between the femoral cam and tibial post. The geometric design of the cam post mechanism determines the kinematics of rollback. The purpose of this study is to optimise the design of the femoral cam-tibial post articulation through finite element analysis and suggest various design parameters that would optim...

  6. FOREIGN CONFLICT REPORTING POST-9/11 AND POST-COLD WAR: A COMPARATIVE ANALYSIS OF EUROPEAN TELEVISION NEWS COVERAGE OF THE MIDDLE EAST CONFLICT

    OpenAIRE

    Heywood, Emma

    2014-01-01

    The thesis explores the state of European foreign conflict reporting by public sector broadcasters, post-Cold War and post-9/11. It provides a comparative analysis of the news values of three television news providers from three differing public systems: BBC’s News at 10, representing a British public service broadcaster, nominally independent of government control; Russia’s Vremya on Channel 1, a state-aligned broadcaster used, to a large extent, as a mouthpiece for the government; and Franc...

  7. Rigid-Plastic Post-Buckling Analysis of Columns and Quadratic Plates

    DEFF Research Database (Denmark)

    Jönsson, Jeppe

    2008-01-01

    The objective of this paper is to show the application of a novel approach to the rigid plastic hinge and yield line theory in post-buckling analysis of slender plates and columns. The upper bound theorem of plasticity theory and the associated flow law of plasticity are used to find the...... yield lines accommodate differential rotations of rigid parts and the area “collapse” yield lines accommodate local area changes of the rigid parts thereby preserving compatibility of the rigid parts of a plate. The approach will be illustrated for rigid plastic column analysis and for a quadratic plate...

  8. STRESS ANALYSIS OF INJECTION MOLDED PARTS IN POST-FILLING STAGE

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The linear isothermo-viscoelastic constitutive equation is established according to the principle of viscoelastic mechanics. Given the boundary conditions of the temperature field, the linear themo-viscoelastic constitutive equation is established acording to the analysis of the thermorheologically simple. The stress analysis model is constructed on the base of some reasonable hypotheses which consider the restraint conditions of mold and the characteristics of injection molding in the post-filling stage. The mathematical model is calculated by the finite difference method. The results can help to predict the warpage of plastic products.

  9. Survival analysis: a tool in the study of post-harvest diseases in peaches

    OpenAIRE

    Cristiano Nunes Nesi; Silvia Emiko Shimakura; Paulo Justiniano Ribeiro Junior; Louise Larissa May De Mio

    2015-01-01

    Survival analysis is applied when the time until the occurrence of an event is of interest. Such data are routinely collected in plant diseases, although applications of the method are uncommon. The objective of this study was to use two studies on post-harvest diseases of peaches, considering two harvests together and the existence of random effect shared by fruits of a same tree, in order to describe the main techniques in survival analysis. The nonparametric Kaplan-Meier method, the log-ra...

  10. Training department's role in human factor analysis during post-trip reviews

    International Nuclear Information System (INIS)

    Provide training is a frequent corrective action specified in a post-trip review report. This corrective action is most often decided upon by technical and operational staff, not training staff, without a detailed analysis of whether training can resolve the immediate problem or enhance employees' future performance. A more specific human factor or performance problem analysis would often reveal that training cannot impact or resolve the concern to avoid future occurrences. This human factor analysis is similar to Thomas Gilbert's Behavior Engineering Model (Human Competence, McGraw-Hill, 1978) or Robert Mager's/Peter Pipe's Performance Analysis (Analyzing Performance Problems, Pitman Learning, 1984). At Palo Verde Nuclear Generating Station, training analysts participate in post-trip reviews in order to conduct or provide input to this type of human factor and performance problem analysis. Their goal is to keep provide training out of corrective action statements unless training can in fact impact or resolve the problem. The analysts follow a plant specific logic diagram to identify human factors and to identify whether changes to the environment or to the person would best resolve the concern

  11. Post-mortem tissue biopsies obtained at minimally invasive autopsy: an RNA-quality analysis.

    Directory of Open Access Journals (Sweden)

    Anita van der Linden

    Full Text Available Bereaved relatives often refuse to give consent for post-mortem investigation of deceased cancer patients, mainly because of the mutilation due to conventional autopsy (CA. Minimally invasive autopsy (MIA may be a more acceptable alternative and, if implemented in clinical practice, creates an opportunity to more often obtain post-mortem tissue samples of (recurred primary tumors and metastases for molecular research. As a measure for tissue quality for molecular studies, we hereby present a feasibility study, comparing the RNA quality of MIA and CA samples, and fresh frozen samples as reference.Tissue samples of heart, liver and kidney were prospectively collected from 24 MIAs followed by CA, and compared to corresponding archival fresh frozen tissue. After RNA isolation and RT-qPCR, RNA integrity numbers (RIN and GAPDH expression (six amplicon sizes ranging from 71 to 530 base pairs were measured. RIN values and GAPDH Cq values were analyzed and compared between all sample groups and post-mortem intervals (PMI.RIN values in MIA samples were significantly higher than those in CA samples. GAPDH was expressed significantly higher in MIA samples than in CA samples and 530 bp PCR products could be measured in all cases. GAPDH expression was significantly lower in samples with PMI >15 hours. As expected, the samples of the fresh frozen reference standard performed best in all analyses.MIA samples showed better RNA quality than CA samples, probably due to shorter PMI. Both had lower RNA quality and expression levels than fresh frozen tissue, however, remaining GAPDH RNA was still sufficiently intact. Therefore, other highly expressed genes are most likely also detectable. Gene array analysis should be performed to gain insight into the quality of entire post-mortem genomes. Reducing PMI will further improve the feasibility of demanding molecular research on post-mortem tissues, this is most likely more feasible with MIA than CA.

  12. Clinical analysis of post-irradiation sensorineural hearing loss in patients suffering from nasopharyngeal carcinoma

    International Nuclear Information System (INIS)

    Objective: To investigate the incidence of post-irradiation sensorineural hearing loss (SNHL) in patients suffering from nasopharyngeal carcinoma and to evaluate its potentially contributing factors. Methods: Pure tonetest and impedance audiography were carried out in patients suffering from nasopharyngeal carcinoma with a post-irradiation follow-up time over 1 year. Additionally, the test results were combined with clinical data and analyzed retrospectively. Results: The follow-up time of all patients ranged from 12 to 94 months (median 53 months). The incidences of SNHL at low and high frequencies were 8% and 42% respectively. Univariate analysis showed that patient's age and follow-up time affected the incidence of SNHL at high frequencies (t=2.051, P=0.0269; t=2.978, P=0.0011), but sex, preirradiation subjective hearing loss, irradiation dose and chemotherapy including cisplatin had no significance. Multivariate analysis by Binary Logistic Regression revealed that the risk of SNHL was correlated with patient's age and follow-up time (P=0.02; P=0.009). Conclusion: Post-irradiation SNHL at high frequencies in patients suffering from nasopharyngeal carcinoma is more common than that at low frequencies. The independent prognostic factors for development of SNHL at high frequencies are patient's age and follow-up time. But the role of preirradiation hearing level ,irradiation dose and chemotherapy including cisplatin are not conclusive and further research is needed. (authors)

  13. Evaluation of Trichodysplasia Spinulosa-Associated Polyomavirus Capsid Protein as a New Carrier for Construction of Chimeric Virus-Like Particles Harboring Foreign Epitopes

    Directory of Open Access Journals (Sweden)

    Alma Gedvilaite

    2015-07-01

    Full Text Available Recombinant virus-like particles (VLPs represent a promising tool for protein engineering. Recently, trichodysplasia spinulosa-associated polyomavirus (TSPyV viral protein 1 (VP1 was efficiently produced in yeast expression system and shown to self-assemble to VLPs. In the current study, TSPyV VP1 protein was exploited as a carrier for construction of chimeric VLPs harboring selected B and T cell-specific epitopes and evaluated in comparison to hamster polyomavirus VP1 protein. Chimeric VLPs with inserted either hepatitis B virus preS1 epitope DPAFR or a universal T cell-specific epitope AKFVAAWTLKAAA were produced in yeast Saccharomyces cerevisiae. Target epitopes were incorporated either at the HI or BC loop of the VP1 protein. The insertion sites were selected based on molecular models of TSPyV VP1 protein. The surface exposure of the insert positions was confirmed using a collection of monoclonal antibodies raised against the intact TSPyV VP1 protein. All generated chimeric proteins were capable to self-assemble to VLPs, which induced a strong immune response in mice. The chimeric VLPs also activated dendritic cells and T cells as demonstrated by analysis of cell surface markers and cytokine production profiles in spleen cell cultures. In conclusion, TSPyV VP1 protein represents a new potential carrier for construction of chimeric VLPs harboring target epitopes.

  14. A Human reliability analysis of post-accident human errors in the PSA of KSNP

    International Nuclear Information System (INIS)

    Korea Atomic Energy Research Institute, using the ASME PRA Standard, evaluated the PSA model of the Korea Standard Nuclear Power Plant (KSNP) and identified the items to be improved to enhance its quality. The new risk monitor PSA model for the KSNP of which quality was enhanced is called as PRiME-U3i. The evaluation results of human reliability analysis (HRA) of the post-accident human errors in the PSA model of the KSNP showed that 10 items among 19 items of supporting requirements for those in the ASME PRA Standard were identified as them to be improved. Thus, we newly carried out a HRA for post-accident human errors for the KSNP PSA model as the target of grading its quality above ASME PRA Standard Category I+. Following tasks were additionally major tasks performed in the HRA of post-accident human errors of PRiME-U3i compared with the previous PSA model of the KSNP: interviews with operators in the collection and review of input data need for the HRA, modeling of initial feed and bleed operation as human actions according to 5 initiating event groups, documentation of information of all the input and bases for the detailed quantifications using the quantification sheets The number of the re-estimated human errors of PRiME-U3i according to the Korea Standard HRA method is 92. Among them, the number of individual post-accident human errors is 55. The number of dependent post-accident human errors is 37. Modeling of one event for initial feed and bleed operation increase the CDF of PRiME-U3i by 13.58% compared with baseline CDF. The assessment results for the new HRA results of post-accident human errors of PRiME-U3i using the ASME PRA Standard show that above 80% items of its supporting requirements for post-accident human errors were graded as its Category II. Thus, this study results sufficiently meet the ASME PRA Standard Category I+. It is expected that the HRA results presented in this study will be greatly helpful to improve the PSA quality for the

  15. Study of cancer-specific chimeric promoters induced by irradiation

    International Nuclear Information System (INIS)

    Objective: To combine the radio-inducible CArG element with cancer-specific human telomerase reverse transcriptase (hTERT) gene promoter, and to construct the novel chimeric promoters. Methods: The synthetic hTERT promoters containing different number of radio-inducible CArG elements were constructed, and the activities of the promoters in the cancer cells (HeLa, A549, and MHCC97 cells) and nomal cells (hEL cells) were detected by using luciferase-reporter assays after the treatment of irradiation (a single or fractionated irradiation dose). Results: Synthetic promoter containing 6 repeated CArG units was better in radio-inducibility than any other promoters containing different number of CArG units, and nearly maximum levels obtained at 4-6 Gy. The very low activities of the chimeric promoters could be detected in normal hEL cells. A similar level of reporter gene expression was observed after 3 fractionated doses of 2 Gy compared with a single dose of 6 Gy in cancer cells. Conclusions: The cancer-specific chimeric promoter containing 6 CArG elements showes the best radio-response, and the chimeric promoter system has the potential in cancer gene therapy. (authors)

  16. Virulence, immunogenicity and vaccine properties of a novel chimeric pestivirus

    DEFF Research Database (Denmark)

    Rasmussen, Thomas Bruun; Uttenthal, Åse; Reimann, Ilona;

    2007-01-01

    avirulent and neither chimeric virus nor viral RNA was detected in serum after vaccination. The safety of the vaccine was tested by horizontal transmission to sentinel pigs, which remained uninfected. The vaccine efficacy was examined by challenge infection with classical swine fever virus (CSFV) Eystrup...

  17. Post annealing performance evaluation of printable interdigital capacitive sensors by principal component analysis

    KAUST Repository

    Zia, Asif Iqbal

    2015-06-01

    The surface roughness of thin-film gold electrodes induces instability in impedance spectroscopy measurements of capacitive interdigital printable sensors. Post-fabrication thermodynamic annealing was carried out at temperatures ranging from 30 °C to 210 °C in a vacuum oven and the variation in surface morphology of thin-film gold electrodes was observed by scanning electron microscopy. Impedance spectra obtained at different temperatures were translated into equivalent circuit models by applying complex nonlinear least square curve-fitting algorithm. Principal component analysis was applied to deduce the classification of the parameters affected due to the annealing process and to evaluate the performance stability using mathematical model. Physics of the thermodynamic annealing was discussed based on the surface activation energies. The post anneal testing of the sensors validated the achieved stability in impedance measurement. © 2001-2012 IEEE.

  18. Monitoring Post Disturbance Forest Regeneration with Hierarchical Object-Based Image Analysis

    Directory of Open Access Journals (Sweden)

    L. Monika Moskal

    2013-10-01

    Full Text Available The main goal of this exploratory project was to quantify seedling density in post fire regeneration sites, with the following objectives: to evaluate the application of second order image texture (SOIT in image segmentation, and to apply the object-based image analysis (OBIA approach to develop a hierarchical classification. With the utilization of image texture we successfully developed a methodology to classify hyperspatial (high-spatial imagery to fine detail level of tree crowns, shadows and understory, while still allowing discrimination between density classes and mature forest versus burn classes. At the most detailed hierarchical Level I classification accuracies reached 78.8%, a Level II stand density classification produced accuracies of 89.1% and the same accuracy was achieved by the coarse general classification at Level III. Our interpretation of these results suggests hyperspatial imagery can be applied to post-fire forest density and regeneration mapping.

  19. Post-Buckling and Ultimate Strength Analysis of Stiffened Composite Panel Base on Progressive Damage

    Science.gov (United States)

    Zhang, Guofan; Sun, Xiasheng; Sun, Zhonglei

    Stiffened composite panel is the typical thin wall structure applied in aerospace industry, and its main failure mode is buckling subjected to compressive loading. In this paper, the development of an analysis approach using Finite Element Method on post-buckling behavior of stiffened composite structures under compression was presented. Then, the numerical results of stiffened panel are obtained by FE simulations. A thorough comparison were accomplished by comparing the load carrying capacity and key position strains of the specimen with test. The comparison indicates that the FEM results which adopted developed methodology could meet the demand of engineering application in predicting the post-buckling behavior of intact stiffened structures in aircraft design stage.

  20. CONSTRUCTION AND EXPRESSION OF A HUMAN-MOUSE CHIMERIC ANTIBODY AGAINST HUMAN BLADDER CANCER

    Institute of Scientific and Technical Information of China (English)

    白银; 王琰; 周丽君; 俞莉章

    2001-01-01

    To construct and express a human-mouse chimeric antibody against human bladder cancer. Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR. A human-mouse chimeric antibody expression vector was constructed and transfected into CHO cells. The chimeric antibody against bladder cancer was expressed and characterized. Result: Eukaryotic expression vector of the chimeric antibody against human bladder carcinoma was successfully constructed, and was expressed in eukaryotic cells; the expressed chimeric antibody ch-BDI showed same specificity as its parent McAb against human bladder cancer cells. Conclusion: The constructed chimeric antibody was expressed successfully in eukaryotic cells, and the chimeric antibody had desired affinity against human bladder cancer cells.

  1. Design and Construction of Chimeric VP8-S2 Antigen for Bovine Rotavirus and Bovine Coronavirus

    Science.gov (United States)

    Nasiri, Khadijeh; Nassiri, Mohammadreza; Tahmoorespur, Mojtaba; Haghparast, Alireza; Zibaee, Saeed

    2016-01-01

    Purpose: Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn calves and in some other species such as pigs and sheep. Rotavirus VP8 subunit is the major determinant of the viral infectivity and neutralization. Spike glycoprotein of coronavirus is responsible for induction of neutralizing antibody response. Methods: In the present study, several prediction programs were used to predict B and T-cells epitopes, secondary and tertiary structures, antigenicity ability and enzymatic degradation sites. Finally, a chimeric antigen was designed using computational techniques. The chimeric VP8-S2 antigen was constructed. It was cloned and sub-cloned into pGH and pET32a(+) expression vector. The recombinant pET32a(+)-VP8-S2 vector was transferred into E.oli BL21CodonPlus (DE3) as expression host. The recombinant VP8-S2 protein was purified by Ni-NTA chromatography column. Results: The results of colony PCR, enzyme digestion and sequencing showed that the VP8-S2 chimeric antigen has been successfully cloned and sub-cloned into pGH and pET32a(+).The results showed that E.coli was able to express VP8-S2 protein appropriately. This protein was expressed by induction of IPTG at concentration of 1mM and it was confirmed by Ni–NTA column, dot-blotting analysis and SDS-PAGE electrophoresis. Conclusion: The results of this study showed that E.coli can be used as an appropriate host to produce the recombinant VP8-S2 protein. This recombinant protein may be suitable to investigate to produce immunoglobulin, recombinant vaccine and diagnostic kit in future studies after it passes biological activity tests in vivo in animal model and or other suitable procedure. PMID:27123423

  2. RICE IN COLONIAL AND POST COLONIAL SOUTHEAST ASIA: A FOOD REGIME ANALYSIS

    OpenAIRE

    Dias Pradadimara

    2015-01-01

    This paper traces the ways in which rice, as a global commodity, has been produced and sold in various regions in Southeast Asia from the colonial era to the present days. This paper employs a food regime analysis first introduced by Harriet Friedmann (1982) and later developed together with Philip McMichael (1989) to look at the global political economy of rice. In this paper, it will be shown how various colonial and post colonial states in Southeast Asia (including Thailand who was never f...

  3. Lesson Learned from AGILE and LARES ASI Projects About MATED Data Collection and Post Analysis

    Science.gov (United States)

    Carpentiero, Rita; Mrchetti, Ernesto; Natalucci, Silvia; Portelli, Claudio

    2012-07-01

    ASI has managed and collected data on project development of two scientific all-Italian missions: AGILE and LARES. Collection of the Model And Test Effectiveness Database (MATED) data, concerning Project, AIV (Assembly Integration and Verification) and NCR (Non Conformance Report) aspects has been performed by the Italian Space Agency (ASI), using available technical documentation of both AGILE e LARES projects. In this paper some consideration on the needs of 'real time' data collection is made, together with proposal of front end improvement of this tool. In addition a preliminary analysis of MATED effectiveness related to the above ASI projects will be presented in a bottom-up and post verification approach.

  4. Procedure for the record, calculation and analysis of costs at the Post Company of Cuba.

    Directory of Open Access Journals (Sweden)

    María Luisa Lara Zayas

    2012-12-01

    Full Text Available The Cuban Company is immersed in important changes, which lead to a new economic model that requires to increase the productivity of work and to enlarge the economic efficiency by means of rational use of material resources, financial and humans. In the present work it is proposed a procedure based on the application of cost techniques, for the record, calculation and costs analysis of activities in the Post Company of Cuba in Sancti Spiritus with the objective to obtain a major efficiency from the rational use of resources.

  5. Data handling and post-reconstruction analysis at next generation experiments

    International Nuclear Information System (INIS)

    A new generation of experiments in high energy physics is approaching. With the approval of the LHC at CERN and the revised Main Injector project at Fermilab, high statistics experiments will start operation within 5 to 10 years. With luminosities Up to 1034/cm2/sec and several hundred thousand readout channels, data most likely cannot be handled and analysed using traditional HEP approaches. The CAP group at Fermilab is investigating different approaches to data handling and organization for post-reconstruction analysis. We discuss the approaches considered, their strengths and weaknesses, integration with hierarchical storage, and sharing of primary data resources

  6. Post-stroke infection: A systematic review and meta-analysis

    Directory of Open Access Journals (Sweden)

    Dijkgraaf Marcel G

    2011-09-01

    Full Text Available Abstract Background stroke is the main cause of disability in high-income countries, and ranks second as a cause of death worldwide. Patients with acute stroke are at risk for infections, but reported post-stroke infection rates vary considerably. We performed a systematic review and meta-analysis to estimate the pooled post-stroke infection rate and its effect on outcome. Methods MEDLINE and EMBASE were searched for studies on post-stroke infection. Cohort studies and randomized clinical trials were included when post-stroke infection rate was reported. Rates of infection were pooled after assessment of heterogeneity. Associations between population- and study characteristics and infection rates were quantified. Finally, we reviewed the association between infection and outcome. Results 87 studies were included involving 137817 patients. 8 studies were restricted to patients admitted on the intensive care unit (ICU. There was significant heterogeneity between studies (P 2 = 97%. The overall pooled infection rate was 30% (24-36%; rates of pneumonia and urinary tract infection were 10% (95% confidence interval [CI] 9-10% and 10% (95%CI 9-12%. For ICU studies, these rates were substantially higher with 45% (95% CI 38-52%, 28% (95%CI 18-38% and 20% (95%CI 0-40%. Rates of pneumonia were higher in studies that specifically evaluated infections and in consecutive studies. Studies including older patients or more females reported higher rates of urinary tract infection. Pneumonia was significantly associated with death (odds ratio 3.62 (95%CI 2.80-4.68. Conclusions Infection complicated acute stroke in 30% of patients. Rates of pneumonia and urinary tract infection after stroke were 10%. Pneumonia was associated with death. Our study stresses the need to prevent infections in patients with stroke.

  7. Investigations of water escape ways in post-floatation basins by determining water microchemism using activation analysis

    International Nuclear Information System (INIS)

    The article deals with a method of investigating water escapes from post-floatation basins. The method is based on differences that occur in the composition of microelements of post-floatation and ground waters in the area of the basins. Some characteristic chemical elements found to occur in post-floatation waters have been used as natural markers for determining their escape ways. The determinations of the selected microelements in water have been made by means of both reactor activation analysis and X-ray fluorescence analysis, with radioisotope induction. (author)

  8. Three-dimensional finite element analysis of stress distribution in composite resin cores with fiber posts of varying diameters.

    Science.gov (United States)

    Okamoto, Kazuhiko; Ino, Teruno; Iwase, Naoki; Shimizu, Eitaroh; Suzuki, Megumi; Satoh, Goh; Ohkawa, Shuji; Fujisawa, Masanori

    2008-01-01

    Using three-dimensional finite element analysis (3D-FEA), stress distributions in the remaining radicular tooth structure were investigated under the condition of varying diameters of fiber post for fiber post-reinforced composite resin cores (fiber post and core) in maxillary central incisors. Four 3D-FEA models were constructed: (1) fiber post (ø1.2, ø1.4, and ø1.6 mm) and composite resin core; and (2) gold-cast post and core. Maximum stresses in the tooth structure for fiber post and core were higher than that for gold-cast post and core. In the former models, stresses in the tooth structure as well as in the composite resin were slightly reduced with increase in fiber post diameter. These results thus suggested that to reduce stress in the remaining radicular tooth with a large coronal defect, it is recommended to accompany a composite resin core with a fiber post of a large diameter. PMID:18309611

  9. Ex post power economic analysis of record of decision operational restrictions at Glen Canyon Dam.

    Energy Technology Data Exchange (ETDEWEB)

    Veselka, T. D.; Poch, L. A.; Palmer, C. S.; Loftin, S.; Osiek, B; Decision and Information Sciences; Western Area Power Administration

    2010-07-31

    On October 9, 1996, Bruce Babbitt, then-Secretary of the U.S. Department of the Interior signed the Record of Decision (ROD) on operating criteria for the Glen Canyon Dam (GCD). Criteria selected were based on the Modified Low Fluctuating Flow (MLFF) Alternative as described in the Operation of Glen Canyon Dam, Colorado River Storage Project, Arizona, Final Environmental Impact Statement (EIS) (Reclamation 1995). These restrictions reduced the operating flexibility of the hydroelectric power plant and therefore its economic value. The EIS provided impact information to support the ROD, including an analysis of operating criteria alternatives on power system economics. This ex post study reevaluates ROD power economic impacts and compares these results to the economic analysis performed prior (ex ante) to the ROD for the MLFF Alternative. On the basis of the methodology used in the ex ante analysis, anticipated annual economic impacts of the ROD were estimated to range from approximately $15.1 million to $44.2 million in terms of 1991 dollars ($1991). This ex post analysis incorporates historical events that took place between 1997 and 2005, including the evolution of power markets in the Western Electricity Coordinating Council as reflected in market prices for capacity and energy. Prompted by ROD operational restrictions, this analysis also incorporates a decision made by the Western Area Power Administration to modify commitments that it made to its customers. Simulated operations of GCD were based on the premise that hourly production patterns would maximize the economic value of the hydropower resource. On the basis of this assumption, it was estimated that economic impacts were on average $26.3 million in $1991, or $39 million in $2009.

  10. Comparison of the effect of thermal stresses on tooth-colored posts, cores and tooth structures by finite element analysis

    Directory of Open Access Journals (Sweden)

    Levent Nalbant

    2012-04-01

    Full Text Available Objectives: The aims of this study were to analyze the influence of cold heat flow in all ceramic crown material, composite core, zirconium and glass fiber reinforced composite post materials, resin based luting cement and root dentin; and to compare these two tooth-colored post systems about their temperature and thermal stress distributions.
    Materials and Methods: A 3-dimesional finite element model of maxillary left canine tooth was constructed. All ceramic crown, composite core, tooth dentin, post and bone were modeled. In the first part of this study, initial body temperature was assumed to be 36.5°C and the outer temperature was reduced to 0°C for 5 secs. In the second part, the thermal stress was calculated as a result of temperature change. For the analysis, 7 nodes of the finite element model were selected and heat flow, temperature and thermal stres on these nodes were evaluated.
    Results: Mean temperature value was 15.75 °C for GFRC post model and 15.47 °C for Zr post model. The maximum von Mises stress was obtained at the node C in both post systems. In general, thermal stress was observed on the cervical part of all-ceramic crown and there was an interface between root dentin-composite core and post material. The temperature gradient of the GFRC post was smaller than that of the zirconia post.
    Conclusions: Within the limitation of this study, zirconia posts produced greater stress than GFRC posts. Temperature changes had more effect on the post-cement interface and cervical areas than on the other areas.

  11. Comparison of automated pre-column and post-column analysis of amino acid oligomers

    Science.gov (United States)

    Chow, J.; Orenberg, J. B.; Nugent, K. D.

    1987-01-01

    It has been shown that various amino acids will polymerize under plausible prebiotic conditions on mineral surfaces, such as clays and soluble salts, to form varying amounts of oligomers (n = 2-6). The investigations of these surface reactions required a quantitative method for the separation and detection of these amino acid oligomers at the picomole level in the presence of nanomole levels of the parent amino acid. In initial high-performance liquid chromatography (HPLC) studies using a classical postcolumn o-phthalaldehyde (OPA) derivatization ion-exchange HPLC procedure with fluorescence detection, problems encountered included lengthy analysis time, inadequate separation and large relative differences in sensitivity for the separated species, expressed as a variable fluorescent yield, which contributed to poor quantitation. We have compared a simple, automated, pre-column OPA derivatization and reversed-phase HPLC method with the classical post-column OPA derivatization and ion-exchange HPLC procedure. A comparison of UV and fluorescent detection of the amino acid oligomers is also presented. The conclusion reached is that the pre-column OPA derivatization, reversed-phase HPLC and UV detection produces enhanced separation, improved sensitivity and faster analysis than post-column OPA derivatization, ion-exchange HPLC and fluorescence detection.

  12. Association of HLA and post-schistosomal hepatic disorder: a systematic review and meta-analysis.

    Science.gov (United States)

    Huy, Nguyen Tien; Hamada, Mohamed; Kikuchi, Mihoko; Lan, Nguyen Thi Phuong; Yasunami, Michio; Zamora, Javier; Hirayama, Kenji

    2011-12-01

    Several human genetic variants, HLA antigens and alleles are reportedly linked to post-schistosomal hepatic disorder (PSHD), but the results from these reports are highly inconclusive. In order to estimate overall associations between human genetic variants, HLA antigens, HLA alleles and PSHD, we systematically reviewed and performed a meta-analysis of relevant studies in both post-schistosomal hepatic disorder and post-schistosomal non-hepatic disorder patients. PubMed, Scopus, Google Scholar, The HuGE Published Literature database, Cochrane Library, and manual search of reference lists of articles published before July 2009 were used to retrieve relevant studies. Two reviewers independently selected articles and extracted data on study characteristics and data regarding the association between genetic variants, HLA antigens, HLA alleles and PSHD in the form of 2×2 tables. A meta-analysis using fixed-effects or random-effects models to pooled odds ratios (OR) with corresponding 95% confidence intervals were calculated only if more than one study had investigated particular variation. We found 17 articles that met our eligibility criteria. Schistosoma mansoni and Schistosoma japonicum were reported as the species causing PSHD. Since human genetic variants were only investigated in one study, these markers were not assessed by meta-analysis. Thus, only HLA-genes (a total of 66 HLA markers) were conducted in the meta-analysis. Our meta-analysis showed that human leucocyte antigens HLA-DQB1*0201 (OR=2.64, P=0.018), DQB1*0303 (OR=1.93, P=0.008), and DRB1*0901 (OR=2.14, P=0.002) alleles and HLA-A1 (OR=5.10, P=0.001), A2 (OR=2.17, P=0.005), B5 (OR=4.63, P=0.001), B8 (OR=2.99, P=0.02), and B12 (OR=5.49, P=0.005) serotypes enhanced susceptibility to PSHD, whereas HLA-DQA1*0501 (OR=0.29, P≤0.001) and DQB1*0301 (OR=0.58, P=0.007) were protective factors against the disease. We further suggested that the DRB1*0901-DQB1*0201, DRB1*0901-DQB1*0303 and A1-B8 haplotypes

  13. High affinity mouse-human chimeric Fab against Hepatitis B surface antigen

    Institute of Scientific and Technical Information of China (English)

    Biplab Bose; Navin Khanna; Subrat K Acharya; Subrata Sinha

    2005-01-01

    AIM: Passive immunotherapy using antibody against hepatitis B surface antigen (HBsAg) has been advocated in certain cases of Hepatitis B infection. We had earlier reported on the cloning and expression of a high affinity scFv derived from a mouse monoclonal (5S) against HBsAg. However this mouse antibody cannot be used for therapeutic purposes as it may elicit anti-mouse immune responses. Chimerization by replacing mouse constant domains with human ones can reduce the immunogenicity of this antibody.METHODS: We cloned the VH and VL genes of this mouse antibody; and fused them with CH1 domain of human IgG1 and CL domain of human kappa chain respectively. These chimeric genes were cloned into a phagemid vector. After initial screening using the phage display system, the chimeric Fab was expressed in soluble form in E. Coli.RESULTS: The chimeric Fab was purified from the bacterial periplasmic extract. We characterized the chimeric Fab using several in vitro techniques and it was observed that the chimeric molecule retained the high affinity and specificity of the original mouse monoclonal.This chimeric antibody fragment was further expressed in different strains of E> coli to increase the yield.CONCLUSION: We have generated a mouse-human chimeric Fab against HBsAg without any significant loss in binding and epitope specificity. This chimeric Fab fragment can be further modified to generate a fulllength chimeric antibody for therapeutic uses.

  14. Status of large-scale analysis of post-translational modifications by mass spectrometry

    DEFF Research Database (Denmark)

    Olsen, Jesper V; Mann, Matthias

    2013-01-01

    established, with label-free methods showing particular promise. It is also becoming possible to determine the absolute occupancy or stoichiometry of PTMS sites on a large scale. Powerful software for the bioinformatic analysis of thousands of PTM sites has been developed. However, a complete inventory of......Cellular function can be controlled through the gene expression program but often protein post translations modifications (PTMs) provide a more precisely and elegant mechanism. Key functional roles of specific modification events for instance during the cell cycle have been known for decades, but...... it has only been in the last ten years that mass spectrometry (MS)-based proteomics has begun to reveal the true extent of the PTM universe. In this overview for the special PTM issue in Molecular and Cellular Proteomics, we take stock of where MS-based proteomics stands in the large-scale analysis...

  15. A post-correction modal superposition method for nonlinear dynamic analysis

    International Nuclear Information System (INIS)

    A post-correction algorithm developed for the dynamic analysis of simple nonlinear and hysteretic structures is extended for the analysis of building structures under earthquake loadings. The nonlinearities in the system are moved to the right-hand-side of the equations of motion and treated as a generalized loading. At each time step, the equations of motion for the resulting linear system are solved either numerically using the constant acceleration method, or analytically using modal superposition. The effect of the nonlinear generalized forces is made equivalent to a series of impulse forces acting on the resulting linear system. The method does not require the re-factorization of the effective tangent stiffness matrix at each step of the integration. (author)

  16. Post-buckling and Large Amplitude Free Vibration Analysis of Composite Beams: Simple Intuitive Formulation

    Science.gov (United States)

    Gunda, Jagadish Babu; Venkateswara Rao, Gundabathula

    2016-04-01

    Post-buckling and large amplitude free vibration analysis of composite beams with axially immovable ends is investigated in the present study using a simple intuitive formulation. Geometric nonlinearity of Von-Karman type is considered in the analysis which accounts for mid-plane stretching action of the beam. Intuitive formulation uses only two parameters: the critical bifurcation point and the axial stretching force developed due to membrane stretching action of the beam. Hinged-hinged, clamped-clamped and clamped-hinged boundary conditions are considered. Numerical accuracy of the proposed analytical closed-form solutions obtained from the intuitive formulation are compared to available finite element solutions for symmetric and asymmetric layup schemes of laminated composite beam which indicates the confidence gained on the present formulation.

  17. Design of chimeric antigen receptors with integrated controllable transient functions

    OpenAIRE

    Alexandre Juillerat; Alan Marechal; Jean-Marie Filhol; Julien Valton; Aymeric Duclert; Laurent Poirot; Philippe Duchateau

    2016-01-01

    The ability to control T cells engineered to permanently express chimeric antigen receptors (CARs) is a key feature to improve safety. Here, we describe the development of a new CAR architecture with an integrated switch-on system that permits to control the CAR T-cell function. This system offers the advantage of a transient CAR T-cell for safety while letting open the possibility of multiple cytotoxicity cycles using a small molecule drug.

  18. Design of chimeric antigen receptors with integrated controllable transient functions.

    Science.gov (United States)

    Juillerat, Alexandre; Marechal, Alan; Filhol, Jean-Marie; Valton, Julien; Duclert, Aymeric; Poirot, Laurent; Duchateau, Philippe

    2016-01-01

    The ability to control T cells engineered to permanently express chimeric antigen receptors (CARs) is a key feature to improve safety. Here, we describe the development of a new CAR architecture with an integrated switch-on system that permits to control the CAR T-cell function. This system offers the advantage of a transient CAR T-cell for safety while letting open the possibility of multiple cytotoxicity cycles using a small molecule drug. PMID:26750734

  19. A Simple Methodology for Conversion of Mouse Monoclonal Antibody to Human-Mouse Chimeric Form

    Directory of Open Access Journals (Sweden)

    Vinh T. Dang

    2013-01-01

    Full Text Available Passive immunotherapy has mainly been used as a therapy against cancer and inflammatory conditions. Recent studies have shown that monoclonal antibody-(mAb- based passive immunotherapy is a promising approach to combat virus infection. Specific mouse mAbs can be routinely generated in large amounts with the use of hybridoma technology but these cannot be used for therapy in human beings due to their immunogenicity. Therefore, the development of chimeric and humanized mAbs is important for therapeutic purpose. This is facilitated by a variety of molecular techniques like recombinant DNA technology and the better understanding of the structure and function of antibody. The human-mouse chimeric forms allow detailed analysis of the mechanism of inhibition and the potential for therapeutic applications. Here, a step-by-step description of the conversion process will be described. The commercial availability of the reagents required in each step means that this experimentation can be easily set up in research laboratories.

  20. Chimeric piggyBac transposases for genomic targeting in human cells.

    Science.gov (United States)

    Owens, Jesse B; Urschitz, Johann; Stoytchev, Ilko; Dang, Nong C; Stoytcheva, Zoia; Belcaid, Mahdi; Maragathavally, Kommineni J; Coates, Craig J; Segal, David J; Moisyadi, Stefan

    2012-08-01

    Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required. The piggyBac (PB) transposase is an efficient gene transfer vector active in a variety of cell types and proven to be amenable to modification. Here we present the design and validation of chimeric PB proteins fused to the Gal4 DNA binding domain with the ability to target transgenes to pre-determined sites. Upstream activating sequence (UAS) Gal4 recognition sites harbored on recipient plasmids were preferentially targeted by the chimeric Gal4-PB transposase in human cells. To analyze the ability of these PB fusion proteins to target chromosomal locations, UAS sites were randomly integrated throughout the genome using the Sleeping Beauty transposon. Both N- and C-terminal Gal4-PB fusion proteins but not native PB were capable of targeting transposition nearby these introduced sites. A genome-wide integration analysis revealed the ability of our fusion constructs to bias 24% of integrations near endogenous Gal4 recognition sequences. This work provides a powerful approach to enhance the properties of the PB system for applications such as genetic engineering and gene therapy. PMID:22492708

  1. The identification of a spontaneous 47, XX, +21/46, XY chimeric fetus with male genitalia

    Directory of Open Access Journals (Sweden)

    Lee Kuei-Fang

    2012-09-01

    Full Text Available Abstract Background Approximately 30 sex-chromosome discordant chimera cases have been reported to date, of which only four cases carried trisomy 21. Here, we present an additional case, an aborted fetus with a karyotype of 47,XX, +21/46,XY. Case presentation Autopsy demonstrated that this fetus was normally developed and had male genitalia. Major characteristics of Down syndrome were not observed except an enlarged gap between the first and second toes. Karyotyping of tissues cultured from the fetus revealed the same chimeric chromosomal composition detected in the amniotic fluid but with a different ratio of [47,XX,+21] to [46,XY]. Further short tandem repeat analysis indicated a double paternal contribution and single maternal contribution to the fetus, with the additional chromosome 21 in the [47,XX,+21] cell lineage originating from the paternal side. Conclusion We thus propose that this chimeric fetus was formed via the dispermic fertilization of a parthenogenetic ovum with one (Y sperm and one (X,+21 sperm.

  2. Data analysis methods for assessing palliative care interventions in one-group pre–post studies

    Science.gov (United States)

    Ioroi, Takeshi; Kakuma, Tatsuyuki; Sakashita, Akihiro; Miki, Yuki; Ohtagaki, Kanako; Fujiwara, Yuka; Utsubo, Yuko; Nishimura, Yoshihiro; Hirai, Midori

    2015-01-01

    Objectives: Studies of palliative care are often performed using single-arm pre–post study designs that lack causal inference. Thus, in this study, we propose a novel data analysis approach that incorporates risk factors from single-arm studies instead of using paired t-tests to assess intervention effects. Methods: Physical, psychological and social evaluations of eligible cancer inpatients were conducted by a hospital-based palliative care team. Quality of life was assessed at baseline and after 7 days of symptomatic treatment using the European Organization for Research and Treatment of Cancer QLQ-C15-PAL. Among 35 patients, 9 were discharged within 1 week and 26 were included in analyses. Structural equation models with observed measurements were applied to estimate direct and indirect intervention effects and simultaneously consider risk factors. Results: Parameters were estimated using full models that included associations among covariates and reduced models that excluded covariates with small effects. The total effect was calculated as the sum of intervention and covariate effects and was equal to the mean of the difference (0.513) between pre- and post-intervention quality of life (reduced model intervention effect, 14.749; 95% confidence intervals, −4.407 and 33.905; p = 0.131; covariate effect, −14.236; 95% confidence interval, −33.708 and 5.236; p = 0.152). Conclusion: Using the present analytical method for single-arm pre–post study designs, factors that modulate effects of interventions were modelled, and intervention and covariate effects were distinguished based on structural equation model. PMID:27092261

  3. Quantitative videofluoroscopic analysis of penetration-aspiration in post-stroke patients

    Directory of Open Access Journals (Sweden)

    Bingjie Li

    2010-01-01

    Full Text Available Background : Dysphagia is a common complication of stroke and is a potential cause for aspiration and malnutrition and is also associated with poor outcome. Videofluoroscopic Swallowing Study (VFSS is the most objective method for evaluation of swallowing disorders. Aim : To investigate the incidence and characteristics of penetration-aspiration in post-stroke patients, and to study the relationship between penetration-aspiration and kinematic parameters of swallow. Materials and Methods : We prospectively studied swallowing function in 105 consecutive post-stroke patients and 100 normal adults by videofluoroscopic swallowing studies. The severity of airway invasion, penetration-aspiration, was studied quantitatively and kinematic parameters of swallow i.e. oral transit time, pharyngeal transit time (PTT, pharyngeal delay timem (PDT, maximal extent of vertical and anterior movement of larynx and hyoid bone for four kinds of boluses were also studied. Logistic regression was used to analyze the association between aspiration and kinematic parameters of swallow. Results : Stroke patients scored significantly higher scores on penetration-aspiration scale than the normal subjects (P < 0.001 during four bolus swallows. Logistic regression analysis showed that PTT, PDT, maximal extent of vertical laryngeal and hyoid movement were statistically associated with the prevalence of aspiration (P < 0.05. Conclusion : Penetration-aspiration is common in stroke patients. Several kinematic parameters of swallow are associated with the presence of aspiration on fluoroscopy. These data demonstrate that VFSS may be helpful for objective identification of dysphagia in stroke patients.

  4. Sharing Feelings Online: Studying Emotional Well-Being via Automated Text Analysis of Facebook Posts

    Directory of Open Access Journals (Sweden)

    Michele eSettanni

    2015-07-01

    Full Text Available Digital traces of activity on social network sites represent a vast source of ecological data with potential connections with individual behavioral and psychological characteristics. The present study investigates the relationship between user-generated textual content shared on Facebook and emotional well-being. Self-report measures of depression, anxiety and stress were collected from 201 adult Facebook users from North Italy. Emotion-related textual indicators, including emoticon use, were extracted form users’ Facebook posts via automated text analysis. Correlation analyses revealed that individuals with higher levels of depression, anxiety expressed negative emotions on Facebook more frequently. In addition, use of emoticons expressing positive emotions correlated negatively with stress level. When comparing age groups, younger users reported higher frequency of both emotion-related words and emoticon use in their posts. Also, the relationship between online emotional expression and self-report emotional well-being was generally stronger in the younger group. Overall, findings support the feasibility and validity of studying individual emotional well-being by means of examination of Facebook profiles. Implications for online screening purposes and future research directions are discussed.

  5. Forest Fires and Post - Fire Regeneration in Algeria Analysis with Satellite Data

    Science.gov (United States)

    Zegrar, Ahmed

    2016-07-01

    The Algerian forests are characterized by a particularly flammable material and fuel. The wind, the relief and the slope facilitates the propagation of fire. The use of remote sensing data multi-­dates, combined with other types of data of various kinds on the environment and forest burned, opens up interesting perspectives for the management of post-­fire regeneration. In this study the use of multi-­temporal remote sensing image Alsat-­1 and Landsat combined with other types of data concerning both background and burned down forest appears to be promising in evaluating and spatial and temporal effects of post fire regeneration. A spatial analysis taking into consideration the characteristics of the burned down site in the North West of Algeria, allowed to better account new factors to explain the regeneration and its temporal and spatial variation. We intended to show the potential use of remote sensing data from satellite ALSAT-­1, of spatial resolution of 32 m. . This approach allows showing the contribution of the data of Algerian satellite ALSAT in the detection and the well attended some forest fires in Algeria.

  6. Sharing feelings online: studying emotional well-being via automated text analysis of Facebook posts.

    Science.gov (United States)

    Settanni, Michele; Marengo, Davide

    2015-01-01

    Digital traces of activity on social network sites represent a vast source of ecological data with potential connections with individual behavioral and psychological characteristics. The present study investigates the relationship between user-generated textual content shared on Facebook and emotional well-being. Self-report measures of depression, anxiety, and stress were collected from 201 adult Facebook users from North Italy. Emotion-related textual indicators, including emoticon use, were extracted form users' Facebook posts via automated text analysis. Correlation analyses revealed that individuals with higher levels of depression, anxiety expressed negative emotions on Facebook more frequently. In addition, use of emoticons expressing positive emotions correlated negatively with stress level. When comparing age groups, younger users reported higher frequency of both emotion-related words and emoticon use in their posts. Also, the relationship between online emotional expression and self-report emotional well-being was generally stronger in the younger group. Overall, findings support the feasibility and validity of studying individual emotional well-being by means of examination of Facebook profiles. Implications for online screening purposes and future research directions are discussed. PMID:26257692

  7. Quantitative Roughness Analysis of Post-harvest Agaricus bisporus by Atomic Force Microscopy

    Institute of Scientific and Technical Information of China (English)

    YANGHong-Shun; FENGGuo-Ping; ANHong-Jie; LIYun-Fei

    2004-01-01

    The moisture loss degree is important in determining the quality of post-harvest mushroom (Agaricus bisporus (Lange) Sing). Quantitative roughness analyzed by atomic force microscopy (AFM) was proposed to denote the degree of shrinkage, with arithmetic average roughness (Ra) and root mean square roughness (Rq) as parameters, The initial value of Ra was (30.035±1.839)nm, while those of 2℃, 25℃ and dynamic temperature on the 2nd day were (40.139±3.359) nm, (54.393±13.534) nm and (41.197±6.555) nm, respectively. There is a similar tendency for the results of Ra and Rq Both values of roughness increased in duration of storage and with increasing temperatures. The three-dimensional profile of the pileus epicutis could signify the process of water evaporation intuitionally. The tendency was in accordance with the roughness results, especially for the earlier stage of the storage (0-2 d). The outcome of roughness analysis could signify the differences of storage conditions. It was shown that the roughness measured by atomic force microscopy effectively reflected the moisture loss degree of the mushroom pileus epicutis during post-harvest storage.

  8. Sharing feelings online: studying emotional well-being via automated text analysis of Facebook posts

    Science.gov (United States)

    Settanni, Michele; Marengo, Davide

    2015-01-01

    Digital traces of activity on social network sites represent a vast source of ecological data with potential connections with individual behavioral and psychological characteristics. The present study investigates the relationship between user-generated textual content shared on Facebook and emotional well-being. Self-report measures of depression, anxiety, and stress were collected from 201 adult Facebook users from North Italy. Emotion-related textual indicators, including emoticon use, were extracted form users’ Facebook posts via automated text analysis. Correlation analyses revealed that individuals with higher levels of depression, anxiety expressed negative emotions on Facebook more frequently. In addition, use of emoticons expressing positive emotions correlated negatively with stress level. When comparing age groups, younger users reported higher frequency of both emotion-related words and emoticon use in their posts. Also, the relationship between online emotional expression and self-report emotional well-being was generally stronger in the younger group. Overall, findings support the feasibility and validity of studying individual emotional well-being by means of examination of Facebook profiles. Implications for online screening purposes and future research directions are discussed. PMID:26257692

  9. The Orion Exploration Flight Test Post Flight Solid Particle Flight Environment Inspection and Analysis

    Science.gov (United States)

    Miller, Joshua E.

    2016-01-01

    Orbital debris in the millimeter size range can pose a hazard to current and planned spacecraft due to the high relative impact speeds in Earth orbit. Fortunately, orbital debris has a relatively short life at lower altitudes due to atmospheric effects; however, at higher altitudes orbital debris can survive much longer and has resulted in a band of high flux around 700 to 1,500 km above the surface of the Earth. While large orbital debris objects are tracked via ground based observation, little information can be gathered about small particles except by returned surfaces, which until the Orion Exploration Flight Test number one (EFT-1), has only been possible for lower altitudes (400 to 500 km). The EFT-1 crew module backshell, which used a porous, ceramic tile system with surface coatings, has been inspected post-flight for potential micrometeoroid and orbital debris (MMOD) damage. This paper describes the pre- and post-flight activities of inspection, identification and analysis of six candidate MMOD impact craters from the EFT-1 mission.

  10. Enhancement by dimethyl myleran of donor type chimerism in murine recipients of bone marrow allografts

    International Nuclear Information System (INIS)

    A major problem in using murine models for studies of bone marrow allograft rejection in leukemia patients is the narrow margin in which graft rejection can be analyzed. In mice irradiated with greater than 9 Gy total body irradiation (TBI) rejection is minimal, whereas after administration of 8 Gy TBI, which spares a significant number of clonable T cells, a substantial frequency of host stem cells can also be detected. In current murine models, unlike in humans, bone marrow allograft rejection is generally associated with full autologous hematopoietic reconstitution. In the present study, we investigated the effect of the myeloablative drug dimethyl myleran (DMM) on chimerism status following transplantation of T cell-depleted allogenic bone marrow (using C57BL/6 donors and C3H/HeJ recipients, conditioned with 8 Gy TBI). Donor type chimerism 1 to 2 months post-transplant of 1 to 3 x 10(6) bone marrow cells was markedly enhanced by using DMM one day after TBI and prior to transplantation. Conditioning with cyclophosphamide instead of DMM, in combination with 8 Gy TBI, did not enhance engraftment of donor type cells. Artificial reconstitution of T cells, after conditioning with TBI plus DMM, by adding mature thymocytes, or presensitization with irradiated donor type spleen cells 1 week before TBI and DMM, led to strong graft rejection and consequently to severe anemia. The anti-donor responses in these models were proportional to the number of added T cells and to the number of cells used for presensitization, and they could be neutralized by increasing the bone marrow inoculum

  11. Electroencephalography reactivity for prognostication of post-anoxic coma after cardiopulmonary resuscitation: A comparison of quantitative analysis and visual analysis.

    Science.gov (United States)

    Liu, Gang; Su, Yingying; Jiang, Mengdi; Chen, Weibi; Zhang, Yan; Zhang, Yunzhou; Gao, Daiquan

    2016-07-28

    Electroencephalogram reactivity (EEG-R) is a positive predictive factor for assessing outcomes in comatose patients. Most studies assess the prognostic value of EEG-R utilizing visual analysis; however, this method is prone to subjectivity. We sought to categorize EEG-R with a quantitative approach. We retrospectively studied consecutive comatose patients who had an EEG-R recording performed 1-3 days after cardiopulmonary resuscitation (CPR) or during normothermia after therapeutic hypothermia. EEG-R was assessed via visual analysis and quantitative analysis separately. Clinical outcomes were followed-up at 3-month and dichotomized as recovery of awareness or no recovery of awareness. A total of 96 patients met the inclusion criteria, and 38 (40%) patients recovered awareness at 3-month followed-up. Of 27 patients with EEG-R measured with visual analysis, 22 patients recovered awareness; and of the 69 patients who did not demonstrated EEG-R, 16 patients recovered awareness. The sensitivity and specificity of visually measured EEG-R were 58% and 91%, respectively. The area under the receiver operating characteristic curve for the quantitative analysis was 0.92 (95% confidence interval, 0.87-0.97), with the best cut-off value of 0.10. EEG-R through quantitative analysis might be a good method in predicting the recovery of awareness in patients with post-anoxic coma after CPR. PMID:27181515

  12. Comparing Social Network Analysis of Posts with Counting of Posts as a Measurement of Learners' Participation in Facebook Discussions

    Science.gov (United States)

    Lee, Hye Yeon; Lee, Hyeon Woo

    2016-01-01

    With the currently growing interest in social network services, many college courses use social network services as platforms for discussions, and a number of studies have been conducted on the use of social network analysis to measure students' participation in online discussions. This study aims to demonstrate the difference between counting…

  13. Survival analysis: a tool in the study of post-harvest diseases in peaches

    Directory of Open Access Journals (Sweden)

    Cristiano Nunes Nesi

    2015-02-01

    Full Text Available Survival analysis is applied when the time until the occurrence of an event is of interest. Such data are routinely collected in plant diseases, although applications of the method are uncommon. The objective of this study was to use two studies on post-harvest diseases of peaches, considering two harvests together and the existence of random effect shared by fruits of a same tree, in order to describe the main techniques in survival analysis. The nonparametric Kaplan-Meier method, the log-rank test and the semi-parametric Cox's proportional hazards model were used to estimate the effect of cultivars and the number of days after full bloom on the survival to the brown rot symptom and the instantaneous risk of expressing it in two consecutive harvests. The joint analysis with baseline effect, varying between harvests, and the confirmation of the tree effect as a grouping factor with random effect were appropriate to interpret the phenomenon (disease evaluated and can be important tools to replace or complement the conventional analysis, respecting the nature of the variable and the phenomenon.

  14. Development of a hot cell for post-irradiation analysis of nuclear fuels

    International Nuclear Information System (INIS)

    Post irradiation examinations of nuclear fuels are performed in order to verify their in-service behavior. Examinations are conducted in compact structures called hot cells, designed to attend the different types of tests and analysis for fuel's characterization. The characterization of fuel microstructure is an activity performed in hot cells. Usually, hot cells for microstructural fuel analysis are designed to allow the metallographic and ceramographic samples preparation and after that, microscopical analysis of the fuel's microstructure. Due to the complexity of the foreseen operations, the severe limitations imposed by the available space into the hot cells, the capabilities of the remote manipulation devices, the procedures of radiological protection and the needs to obtain samples with an adequate surface quality for microscopic analysis, the design of a hot cell for fuel samples preparation presents a high level of complexity. In this paper, the methodology used to develop a hot cell facility for nuclear fuel's metallographic and ceramographic samples preparation is presented. Equipment, devices and systems used in conventional sample preparation processes were evaluated during bench tests. After the necessary adjustments and processes adaptations, they were assembled in a mock-up of the respective hot cell, where they were tested in conditions as realistic as possible, in order to improve the operations and processes to be performed at the real hot cells. (author)

  15. Post-test analysis of ROSA-III test Run 701

    International Nuclear Information System (INIS)

    Post-test analysis of ROSA-III test Run 701, the first simulated BWR/LOCA experiment in the ROSA-III program, was made by a computer code, RELAP4J. Objectives of the analysis were to evaluate ability of the code to predict thermal hydraulic behavior of coolant by comparing the analytical results with the test data, to show areas of model refinement and to reveal requirements in the program. Analysis showed the following: 1) Calculation model to give a maximum flow rate at low quality is important in determining coolant behavior in the system. 2) The phase separation model in the lower plenum influences calculation of the discharge quality at the break and hence that of coolant behavior in the system. 3) Overall behavior of coolant can be well predicted. 4) Effect of ECC water cannot be well predicted by RELAP4J. Following are the requirements in future tests: 1) measurement of the initial pressure distribution in the system, 2) improvement or development of two-phase flow instrumentations, especially in flow rate measurement, 3) simplification of the feed water and steam discharge lines. In future analysis, emphasis should be placed on prediction of ECC water behavior, evaluation of heat transfer correlation and realistic evaluation of liquid level in the core. (author)

  16. Construction of an allogenic chimeric mouse model for the study of the behaviors of donor stem cells in vivo

    Institute of Scientific and Technical Information of China (English)

    WANG Mo-lin; YAN Jing-bin; XIAO Yan-ping; HUANG Shu-zhen

    2005-01-01

    Background It is essential to establish an animal model for the elucidation of the biological behaviors of stem cells in vivo. We constructed a chimeric animal model by in utero transplantation for investigation of stem cell transplantation.Methods This chimerism was achieved by injecting the stem cells derived from the bone marrow of green fluorescence protein (GFP)-transgenic mice into fetal mice at 13.5 days of gestation. Several methods such as polymerase chain reaction (PCR), real-time PCR, fluorescence-assisted cell sorting (FACS) and fluorescence in situ hybridization (FISH) were used for the observation of donor cells.Results Under a fluorescence microscope, we observed the GFP cells of donor-origin in a recipient. PCR, FACS analysis and FISH indicated chimerism at various intervals. Real-time PCR indicated that some donor cells existed in chimera for more than 6 months.Conclusions Allogenic stem cells may exist in recipients for a long time and this allogenic animal model provides a useful tool for studying the behavior of hematopoietic stem cells and also offers an effective model system for the study of stem cells.

  17. Chimeric Proteins to Detect DNA Damage and Mismatches

    Energy Technology Data Exchange (ETDEWEB)

    McCutchen-Maloney, S; Malfatti, M; Robbins, K M

    2002-01-14

    The goal of this project was to develop chimeric proteins composed of a DNA mismatch or damage binding protein and a nuclease, as well as methods to detect DNA mismatches and damage. We accomplished this through protein engineering based on using polymerase chain reactions (PCRs) to create chimeras with novel functions for damage and mismatch detection. This project addressed fundamental questions relating to disease susceptibility and radiation-induced damage in cells. It also supported and enhanced LLNL's competency in the emerging field of proteomics. In nature, DNA is constantly being subjected to damaging agents such as exposure to ultraviolet (UV) radiation and various environmental and dietary carcinogens. If DNA damage is not repaired however, mutations in DNA result that can eventually manifest in cancer and other diseases. In addition to damage-induced DNA mutations, single nucleotide polymorphisms (SNPs), which are variations in the genetic sequence between individuals, may predispose some to disease. As a result of the Human Genome Project, the integrity of a person's DNA can now be monitored. Therefore, methods to detect DNA damage, mutations, and SNPs are useful not only in basic research but also in the health and biotechnology industries. Current methods of detection often use radioactive labeling and rely on expensive instrumentation that is not readily available in many research settings. Our methods to detect DNA damage and mismatches employ simple gel electrophoresis and flow cytometry, thereby alleviating the need for radioactive labeling and expensive equipment. In FY2001, we explored SNP detection by developing methods based on the ability of the chimeric proteins to detect mismatches. Using multiplex assays with flow cytometry and fluorescent beads to which the DNA substrates where attached, we showed that several of the chimeras possess greater affinity for damaged and mismatched DNA than for native DNA. This affinity was

  18. Fuel retention in JET ITER-Like Wall from post-mortem analysis

    International Nuclear Information System (INIS)

    Selected Ion Beam Analysis techniques applicable for detecting deuterium and heavier impurities have been used in the post-mortem analyses of tiles removed after the first JET ITER-Like Wall (JET-ILW) campaign. Over half of the retained fuel was measured in the divertor region. The highest figures for fuel retention were obtained from regions with the thickest deposited layers, i.e. in the inner divertor on top of tile 1 and on the High Field Gap Closure tile, which resides deep in the plasma scrape-off layer. Least retention was found in the main chamber high erosion regions, i.e. in the mid-plane of Inner Wall Guard Limiter. The fuel retention values found typically varied with deposition layer thicknesses. The reported retention values support the observed decrease in fuel retention obtained with gas balance experiments of JET-ILW

  19. Fuel retention in JET ITER-Like Wall from post-mortem analysis

    Energy Technology Data Exchange (ETDEWEB)

    Heinola, K., E-mail: kalle.heinola@ccfe.ac.uk [Association EURATOM-TEKES, University of Helsinki, PO Box 64, 00560 Helsinki (Finland); EURATOM/CCFE Fusion Association, Culham Science Centre, Abingdon OX14 3DB (United Kingdom); Widdowson, A. [EURATOM/CCFE Fusion Association, Culham Science Centre, Abingdon OX14 3DB (United Kingdom); Likonen, J. [Association EURATOM-TEKES, VTT, PO Box 1000, 02044 VTT, Espoo (Finland); Alves, E. [Instituto Superior Tecnico, Instituto de Plasmas e Fusao Nuclear, Universidade de Lisboa, 1049-001 Lisboa (Portugal); Baron-Wiechec, A. [EURATOM/CCFE Fusion Association, Culham Science Centre, Abingdon OX14 3DB (United Kingdom); Barradas, N. [Instituto Superior Tecnico, Instituto de Plasmas e Fusao Nuclear, Universidade de Lisboa, 1049-001 Lisboa (Portugal); Brezinsek, S. [Forschungszentrum Julich GmbH, EURATOM Association, D-52425 Julich (Germany); Catarino, N. [Instituto Superior Tecnico, Instituto de Plasmas e Fusao Nuclear, Universidade de Lisboa, 1049-001 Lisboa (Portugal); Coad, P. [EURATOM/CCFE Fusion Association, Culham Science Centre, Abingdon OX14 3DB (United Kingdom); Koivuranta, S. [Association EURATOM-TEKES, VTT, PO Box 1000, 02044 VTT, Espoo (Finland); Matthews, G.F. [EURATOM/CCFE Fusion Association, Culham Science Centre, Abingdon OX14 3DB (United Kingdom); Mayer, M. [Max-Planck Institut fur Plasmaphysik, EURATOM Association, D-85748 Garching (Germany); Petersson, P. [Royal Institute of Technology, Association EURATOM-VR, SE-10044 Stockholm (Sweden)

    2015-08-15

    Selected Ion Beam Analysis techniques applicable for detecting deuterium and heavier impurities have been used in the post-mortem analyses of tiles removed after the first JET ITER-Like Wall (JET-ILW) campaign. Over half of the retained fuel was measured in the divertor region. The highest figures for fuel retention were obtained from regions with the thickest deposited layers, i.e. in the inner divertor on top of tile 1 and on the High Field Gap Closure tile, which resides deep in the plasma scrape-off layer. Least retention was found in the main chamber high erosion regions, i.e. in the mid-plane of Inner Wall Guard Limiter. The fuel retention values found typically varied with deposition layer thicknesses. The reported retention values support the observed decrease in fuel retention obtained with gas balance experiments of JET-ILW.

  20. Non-LTE Spectral Analysis of Extremely Hot Post-AGB Stars: Constraints for Evolutionary Theory

    CERN Document Server

    Rauch, Thomas; Ziegler, Marc; Koesterke, Lars; Kruk, Jeffrey W

    2008-01-01

    Spectral analysis by means of Non-LTE model-atmosphere techniques has arrived at a high level of sophistication: fully line-blanketed model atmospheres which consider opacities of all elements from H to Ni allow the reliable determination of photospheric parameters of hot, compact stars. Such models provide a crucial test of stellar evolutionary theory: recent abundance determinations of trace elements like, e.g., F, Ne, Mg, P, S, Ar, Fe, and Ni are suited to investigate on AGB nucleosynthesis. E.g., the strong Fe depletion found in hydrogen-deficient post-AGB stars is a clear indication of an efficient s-process on the AGB where Fe is transformed into Ni or even heavier trans iron-group elements. We present results of recent spectral analyses based on high-resolution UV observations of hot stars.

  1. Statistical analysis of post mortem DNA damage-derived miscoding lesions in Neandertal mitochondrial DNA

    DEFF Research Database (Denmark)

    Vives, Sergi; Gilbert, M Thomas; Arenas, Conchita;

    2008-01-01

    the Heavy strand could explain the observed bias, a phenomenon that could be further tested with non-PCR based approaches. The characterization of the HVS1 hotspots will be of use to future Neandertal mtDNA studies, with specific regards to assessing the authenticity of new positions previously......ABSTRACT: BACKGROUND: We have analysed the distribution of post mortem DNA damage derived miscoding lesions from the datasets of seven published Neandertal specimens that have extensive cloned sequence coverage over the mitochondrial DNA (mtDNA) hypervariable region 1 (HVS1). The analysis was......-->A miscoding lesions (observed ratio of 67:2 compared to an expected ratio of 7:2), implying that the mtDNA Light strand molecule suffers proportionally more damage-derived miscoding lesions than the Heavy strand. CONCLUSION: The clustering of Cs in the Light strand as opposed to the singleton pattern of Cs in...

  2. Thermo-mechanical Analysis of the CLIC Post-Linac Energy Collimators

    CERN Document Server

    Resta-Lopez, J; Latina, A

    2012-01-01

    The post-linac energy collimation system of the Compact Linear Collider (CLIC) has been designed for passive protection of the Beam Delivery System (BDS) against miss-steered beams due to failure modes in the main linac. In this paper, a thermo-mechanical analysis of the CLIC energy collimators is presented. This study is based on simulations using the codes FLUKA and ANSYS when an entire bunch train hits the collimators. Different failure mode scenarios in the main linac are considered. The aim is to improve the collimator in order to make a reliable and robust design so that survives without damage the impact of a full bunch train in case of likely events generating energy errors.

  3. Contour Cluster Shape Analysis for Building Damage Detection from Post-earthquake Airborne LiDAR

    Directory of Open Access Journals (Sweden)

    HE Meizhang

    2015-04-01

    Full Text Available Detection of the damaged building is the obligatory step prior to evaluate earthquake casualty and economic losses. It's very difficult to detect damaged buildings accurately based on the assumption that intact roofs appear in laser data as large planar segments whereas collapsed roofs are characterized by many small segments. This paper presents a contour cluster shape similarity analysis algorithm for reliable building damage detection from the post-earthquake airborne LiDAR point cloud. First we evaluate the entropies of shape similarities between all the combinations of two contour lines within a building cluster, which quantitatively describe the shape diversity. Then the maximum entropy model is employed to divide all the clusters into intact and damaged classes. The tests on the LiDAR data at El Mayor-Cucapah earthquake rupture prove the accuracy and reliability of the proposed method.

  4. The Regulation of Residential Tenancy Markets in Post-War Western Europe: An Economic Analysis

    Directory of Open Access Journals (Sweden)

    Mora-Sanguinetti, Juan S.

    2011-06-01

    Full Text Available This paper provides an economic analysis of the post-war regulation of European tenancy markets. Two representative types of market regulation are analyzed: the introduction of compulsory duration clauses in tenancy contracts (as a means of protecting the tenant against eviction; and rent control policies. First, the study describes and analyzes the recent history of such regulations in Spain, Italy, Finland and the UK, in order to draw some general conclusions about the evolution of European institutions in recent decades. Their effects are then explored by adapting a theoretical model of tenancy markets. The results show that both rent control and compulsory duration clauses potentially entail negative effects for European tenancy markets as they may drive some participants out of the market. These effects are consistent with the trends observed during the latter half of the 20th century in several European countries.

  5. A pilot DTI analysis in patients with recent onset post-traumatic stress disorder

    Science.gov (United States)

    Liu, Yang; Li, Liang; Li, Baojuan; Zhang, Xi; Lu, Hongbing

    2016-03-01

    To explore the alteration in white matter between survivors with recent onset post-traumatic stress disorder (PTSD) and without PTSD, who survived from the same coal mine flood disaster, the diffusion tensor imaging (DTI) sequences were analyzed using DTI studio and statistical parametric mapping (SPM) packages in this paper. From DTI sequence, the fractional anisotropy (FA) value describes the degree of anisotropy of a diffusion process, while the apparent diffusion coefficient (ADC) value reflects the magnitude of water diffusion. The DTI analyses between PTSD and non-PTSD indicate lower FA values in the right caudate nucleus, right middle temporal gyrus, right fusiform gyrus, and right superior temporal gyrus, and higher ADC values in the right superior temporal gyrus and right corpus callosum of the subjects with PTSD. These results are partly in line with our previous volume and cortical thickness analyses, indicating the importance of multi-modality analysis for PTSD.

  6. Mass spectrometric identification of proteins and characterization of their post-translational modifications in proteome analysis

    DEFF Research Database (Denmark)

    Roepstorff, P; Larsen, Martin Røssel

    2001-01-01

    more than 425.000 protein sequences. However, the cellular functions are determined by the set of proteins expressed in the cell--the proteome. Two-dimensional gel electrophoresis, mass spectrometry and bioinformatics have become important tools in correlating the proteome with the genome. The current...... dominant strategies for identification of proteins from gels based on peptide mass spectrometric fingerprinting and partial sequencing by mass spectrometry are described. After identification of the proteins the next challenge in proteome analysis is characterization of their post-translational...... modifications. The general problems associated with characterization of these directly from gel separated proteins are described and the current state of art for the determination of phosphorylation, glycosylation and proteolytic processing is illustrated....

  7. Error analysis in post linac to driver linac transport beam line of RAON

    Science.gov (United States)

    Kim, Chanmi; Kim, Eun-San

    2016-07-01

    We investigated the effects of magnet errors in the beam transport line connecting the post linac to the driver linac (P2DT) in the Rare Isotope Accelerator in Korea (RAON). The P2DT beam line is bent by 180-degree to send the radioactive Isotope Separation On-line (ISOL) beams accelerated in Linac-3 to Linac-2. This beam line transports beams with multi-charge state 132Sn45,46,47. The P2DT beam line includes 42 quadrupole, 4 dipole and 10 sextupole magnets. We evaluate the effects of errors on the trajectory of the beam by using the TRACK code, which includes the translational and the rotational errors of the quadrupole, dipole and sextupole magnets in the beam line. The purpose of this error analysis is to reduce the rate of beam loss in the P2DT beam line. The distorted beam trajectories can be corrected by using six correctors and seven monitors.

  8. Analysis of Post-Deployment Cognitive Performance and Symptom Recovery in U.S. Marines

    OpenAIRE

    Haran, F. J.; Alphonso, Aimee L.; Alia Creason; Campbell, Justin S.; Dagny Johnson; Emily Young; Tsao, Jack W.

    2013-01-01

    BACKGROUND: Computerized neurocognitive testing (NCAT) has been proposed to be useful as a screening tool for post-deployment cognitive deficits in the setting of mild traumatic brain injury (mTBI). We assessed the clinical utility of post-injury/post-deployment Automated Neurocognitive Assessment Metric (ANAM) testing, using a longitudinal design to compare baseline ANAM tests with two post-deployment ANAM tests in a group of Marines who experienced combat during deployment. METHODS AND FIND...

  9. Brain structure in post-traumatic stress disorder: A voxel-based morphometry analysis

    OpenAIRE

    Tan, Liwen; Zhang, Li; Qi, Rongfeng; Lu, Guangming; Li, Lingjiang; Liu, Jun; Li, Weihui

    2013-01-01

    This study compared the difference in brain structure in 12 mine disaster survivors with chronic post-traumatic stress disorder, 7 cases of improved post-traumatic stress disorder symptoms, and 14 controls who experienced the same mine disaster but did not suffer post-traumatic stress disorder, using the voxel-based morphometry method. The correlation between differences in brain structure and post-traumatic stress disorder symptoms was also investigated. Results showed that the gray matter v...

  10. Chimeric Allografts Induced by Short-Term Treatment With Stem Cell Mobilizing Agents Result in Long-Term Kidney Transplant Survival Without Immunosuppression: II, Study in Miniature Swine.

    Science.gov (United States)

    Cameron, A M; Wesson, R N; Ahmadi, A R; Singer, A L; Hu, X; Okabayashi, T; Wang, Y; Shigoka, M; Fu, Y; Gao, W; Raccusen, L C; Montgomery, R A; Williams, G M; Sun, Z

    2016-07-01

    Transplantation is now lifesaving therapy for patients with end-stage organ failure but requires lifelong immunosuppression with resultant morbidity. Current immunosuppressive strategies inhibit T cell activation and prevent donor-recipient engagement. Therefore, it is not surprising that few host cells are demonstrated in donor grafts. However, our recent small animal studies found large numbers of recipient stem cells present after transplantation and pharmacological mobilization, resulting in a chimeric, repopulated organ. We now confirm these findings in a well-characterized large animal preclinical model. Here, we show that AMD3100 and FK506 mobilization of endogenous stem cells immediately post kidney transplantation combined with repeat therapy at 1, 2, and 3 months led to drug-free long-term survival in maximally immunologically mismatched swine. Three long-term recipients have stable chimeric transplants, preserved antidonor skin graft responses, and normal serum creatinine levels despite withdrawal of all medication for 3 years. PMID:26748958

  11. Post-test analysis of ROSA-III experiment Run 702

    International Nuclear Information System (INIS)

    The purpose of the ROSA-III experiment with a scaled BWR test facility is to examine primary coolant thermal-hydraulic behavior and performance of ECCS during a posturated loss-of-coolant accident of BWR. The results provide information for verification and improvement of reactor safety analysis codes. Run 702 assumed a 200% split break at the recirculation pump suction line under an average core power without ECCS activation. Post - test analysis of the Run 702 experiment was made with computer code RELAP4J. Agreement of the calculated system pressure and the experiment one was good. However, the calculated heater surface temperatures were higher than the measured ones. Also, the axial temperature distribution was different in tendency from the experimental one. From these results, the necessity was indicated of improving the analytical model of void distribution in the core and the nodalization in the pressure vassel, in order to make the analysis more realistic. And also, the need of characteristic test was indicated for ROSA-III test facility components, such as jet pump and piping form loss coefficient; likewise, flow rate measurements must be increased and refined. (author)

  12. Case of 46,XX/47,XY, +21 chimerism in a newborn infant with ambiguous genitalia

    Energy Technology Data Exchange (ETDEWEB)

    Sawai, Tomoko; Yoshimoto, Masaaki; Kinoshita, Ei-ichi; Baba, Tsuneyoshi; Matsumoto, Tadashi; Tsuji, Yoshiro, Niikawa, Norio [Nagasaki Univ. School of Medicine, Nagasaki (Japan); Fukuda, Shinpei [Ohmura Municipal Hospital, Ohmura (Japan); Harada, Naoki [Kyushu Medical Science, Nagasaki (Japan)

    1994-02-15

    The authors describe the whole-body chimerism in a newborn infant with small phallus, pseudo-vaginal perineal hypospadias, and a bifid scrotum containing gonads. The human testis determining factor gene (SRY) was detected by PCR amplification. GTG-banding chromosome analysis in peripheral blood lymphocytes and cultured fibroblasts derived from right cubital skin showed a 46,XX/47,XY, +21 karyotype. Their ratios in each cell line were 294:5 and 178:7, respectively. QFQ-banding chromosome analysis documented 3 heteromorphic satellites on trisomic chromsomes 21 in the 47,XY,+21 cell line and a homozygous satellite pattern in the 46,XX cell line. Heteromorphic patterns of chromsomes 4, 13, 14, and 22 were also different between the two cell lines. To our knowledge, such disomy/trisomy chimeras have not been described previously. 10 refs., 3 figs.

  13. Regional analysis of distribution of pre and post 2015 Nepal Earthquake landslides

    Science.gov (United States)

    Valagussa, Andrea; Frattini, Paolo; Crosta, Giovanni; Valbuzzi, Elena

    2016-04-01

    A magnitude 7.8 earthquake struck Nepal on April 25, 2015. Three landslide inventories have been prepared in four districts: Dhading (1885 km2), Sindhupalchok (2488 km2), Rasuwa (1522 km2) and Nuwakot (1194 km2), that are located north of Kathmandu. These inventories extend 14 to 138 km SE from the epicenter of the main shock (April 25, 2015), 4.5 to 143 km NW from the epicenter of the main aftershock (May 12, 2015), and 34 to 136 km from the Main Frontal Thrust. The first inventory is a coseismic and post-seismic landslide inventory based on multi-temporal images (Google Earth, Google Crisis maps, Bing maps), and helicopter-based video. The second one is a pre-event shallow landslide inventory. In these two inventories the most abundant landslide types are: debris flows, shallow translational slides, and rockfalls. The third is a deep seated landslide inventory, in which the most represented landslide types are rock avalanches, slumps, rockslides and deep-seated gravitational slope deformations (DSGSD). All the landslides have been mapped as individual polygons. For the analysis we focus our attention on four districts: First we studied how the landslide frequency density changes as a function of topographic parameters (i.e. slope gradient, slope aspect, and elevation). The analyses have been based on the ASTER Global Digital Elevation Model (ASTER GDEM). For coseismic and post-seismic landslides we observed that the mean slope gradient at which the landslide occurs is higher with respect to the two other inventories (50° and 30/40° respectively). The slope aspect of coseismic and post-seismic landslides is also different, with a larger frequency of landslides towards SW, whereas in pre-event landslides the most common slope aspect is SE. This could be related to the direction of the seismic wave. At least the coseismic and post-seismic landslides occur, in mean, at an elevation lower than the pre-event landslides. We also analyzed the relationship between the

  14. The need for complementary hydraulic analysis in post-restoration monitoring of river restoration projects

    Directory of Open Access Journals (Sweden)

    T. A. Endreny

    2011-03-01

    Full Text Available River restoration design methods are incrementally improved by studying and learning from monitoring data in previous projects. In this paper, we report post-restoration monitoring data for a Natural Channel Design (NCD restoration project along 1600 m (10 channel wavelengths of the Batavia Kill in the Catskill Mountains, NY, implemented in 2001 and 2002. The NCD project used a reference-reach to determine channel form, empirical relations between the project site and reference site bankfull dimensions to size channel geometry, and hydraulic and sediment computations to test channel capacity and sediment stability. In addition 12 cross-vanes and 48 j-hook vanes used in NCD for river training were installed to protect against bank erosion and maintain scour pools for fish habitat. Changes in pool depths were monitored with surveys from 2002–2004, and then after the channel-altering April 2005 flood. Aggradation in pools was attributed to cross-vane arms not concentrating flow in the center of the channel, which subsequently caused flow splitting and 4 partial point bar avulsions during the 2005 flood. Hydrodynamic simulation at the 18 m3s−1 bankfull flow suggested avulsions occurred where vanes allowed erosive bank scour to initiate the avulsion cut, and once the flow was split, the diminished in-channel flow caused more aggradation in the pools. In this project post-restoration monitoring had detected aggradation and considered it a problem. The lesson for the larger river restoration community is monitoring protocol should include complementary hydraulic and sediment analysis to comprehend potential consequences and develop preventative maintenance. River restoration and monitoring teams should be trained in robust hydraulic and sediment analytical methods that help them extend project restoration goals.

  15. Cultural Identity Expressions through Visual Analysis in Post-Disaster Housing

    Directory of Open Access Journals (Sweden)

    Asal K. Fard

    2010-01-01

    Full Text Available Problem statement: The incidence of natural disasters as well as followed human beings efforts for reestablishing life is considered as unavoidable essence of nature. Whilst, the interdisciplinary theme of post disaster recovery and reconstruction has been researched worldwide in order to examine its different aspects. And within architectural interests, evaluating the housing reconstruction programs would lead to beneficial clues through which useful lessons may be learnt in terms of reconstruction management while facing such unexpected challenges. Approach: This study aimed to present the visual results of the field work survey based on the mixed method research regarding post-Bam 2003 earthquake housing reconstruction efforts. In fact, the whole study was designed to find out the relation between the attitude of survived households toward their new built homes and the degree of their participation in housing reconstruction process. The focus was on analyzing the selected captured photographs provided to support the findings as documentation of research observations. Results: Accordingly, photographs were organized in to certain categories used as a mode of interpretation within the structure of visual research method analysis. The results indicated that despite different meanings of home to households, their active participation in new housing would accelerate the recovery process from sense of loss caused by catastrophes. Besides, hereby the visual and abstract tools and objectives used by households in order to express their feelings and attitude toward new built homes was addressed within defined method. Conclusion: The results indicated that despite various limitations causing change in local housing pattern following Bam earthquake, residents have attempt to express their cultural identity in some degrees as linkage to lost settings.

  16. Chimeras taking shape: potential functions of proteins encoded by chimeric RNA transcripts.

    Science.gov (United States)

    Frenkel-Morgenstern, Milana; Lacroix, Vincent; Ezkurdia, Iakes; Levin, Yishai; Gabashvili, Alexandra; Prilusky, Jaime; Del Pozo, Angela; Tress, Michael; Johnson, Rory; Guigo, Roderic; Valencia, Alfonso

    2012-07-01

    Chimeric RNAs comprise exons from two or more different genes and have the potential to encode novel proteins that alter cellular phenotypes. To date, numerous putative chimeric transcripts have been identified among the ESTs isolated from several organisms and using high throughput RNA sequencing. The few corresponding protein products that have been characterized mostly result from chromosomal translocations and are associated with cancer. Here, we systematically establish that some of the putative chimeric transcripts are genuinely expressed in human cells. Using high throughput RNA sequencing, mass spectrometry experimental data, and functional annotation, we studied 7424 putative human chimeric RNAs. We confirmed the expression of 175 chimeric RNAs in 16 human tissues, with an abundance varying from 0.06 to 17 RPKM (Reads Per Kilobase per Million mapped reads). We show that these chimeric RNAs are significantly more tissue-specific than non-chimeric transcripts. Moreover, we present evidence that chimeras tend to incorporate highly expressed genes. Despite the low expression level of most chimeric RNAs, we show that 12 novel chimeras are translated into proteins detectable in multiple shotgun mass spectrometry experiments. Furthermore, we confirm the expression of three novel chimeric proteins using targeted mass spectrometry. Finally, based on our functional annotation of exon organization and preserved domains, we discuss the potential features of chimeric proteins with illustrative examples and suggest that chimeras significantly exploit signal peptides and transmembrane domains, which can alter the cellular localization of cognate proteins. Taken together, these findings establish that some chimeric RNAs are translated into potentially functional proteins in humans. PMID:22588898

  17. Analysis of a convection loop for GFR post-LOCA decay heat removal

    International Nuclear Information System (INIS)

    A computer code (LOCA-COLA) has been developed at MIT for steady state analysis of convective heat transfer loops. In this work, it is used to investigate an external convection loop for decay heat removal of a post-LOCA gas-cooled fast reactor (GFR). The major finding is that natural circulation cooling of the GFR is feasible under certain circumstances. Both helium and CO2 cooled system components are found to operate in the mixed convection regime, the effects of which are noticeable as heat transfer enhancement or degradation. It is found that CO2 outdoes helium under identical natural circulation conditions. Decay heat removal is found to have a quadratic dependence on pressure in the laminar flow regime and linear dependence in the turbulent flow regime. Other parametric studies have been performed as well. In conclusion, convection cooling loops are a credible means for GFR decay heat removal and LOCA-COLA is an effective tool for steady state analysis of cooling loops. (authors)

  18. Reaction flow chromatography for rapid post column derivatisations: the analysis of antioxidants in natural products.

    Science.gov (United States)

    Camenzuli, M; Ritchie, H J; Dennis, G R; Shalliker, R A

    2013-08-16

    The analysis of antioxidants from complex samples is conveniently achieved using liquid chromatography, which provides sample fraction, coupled with an on-line antioxidant assay, which provides detection. One particularly useful on-line antioxidant assay that has routinely been coupled with HPLC involves the diphenylpicrylhydrazyl radical (DPPH), which provides a positive test for phenolic antioxidants through a decolorisation of the DPPH reagent. A limitation of this assay, however, is the need to employ a reaction coil, which is often large with respect to the peak volume, consequently adding substantial band broadening to the separation. In this study we introduce a new concept that can be employed for systems requiring post column derivatisations, such as the DPPH assay. We have termed this 'reaction flow' chromatography, whereby, the derivatisation reagent can be added directly into one of the outlet ports of a parallel segmented flow column. Subsequently, the mixing between the derivatising reagent and the solute is very efficient removing the need to employ reaction coils. The concept is tested here using the DPPH assay for the analysis of antioxidants in samples derived from natural origin. PMID:23849586

  19. Electromyographic and neuromuscular analysis in patients with post-polio syndrome.

    Science.gov (United States)

    Corrêa, J C F; Rocco, C Chiusoli de Miranda; de Andrade, D Ventura; Peres, J Augusto; Corrêa, F Ishida

    2008-01-01

    Proceed to a comparative analysis of the electromyographic (EMG) activity of the muscles rectus femoris, vastus medialis and vastus lateralis, and to assess muscle strength and fatigue after maximal isometric contraction during knee extension. Eighteen patients with post-polio syndrome, age and weight matched, were utilized in this study. The signal acquisition system utilized consisted of three pairs of surface electrodes positioned on the motor point of the analyzed muscles. It was possible to observe with the results of this study a decreased endurance on initial muscle contraction and during contraction after 15 minutes of the initial maximal voluntary contraction, along with a muscle fatigue that was assessed through linear regression executed with Pearson's test. There were significant differences among the comparative analysis of EMG activity of the muscles rectus femoris, vastus medialis and vastus lateralis after maximal isometric contraction during knee extension. Initial muscle contraction and contraction after a 15 minute-rest from initial contraction decreased considerably, indicating a decreased endurance on muscle contraction, concluding that a lower limb muscle fatigue was present on the analyzed PPS patients. PMID:19097472

  20. Analysis of Solar Wind Samples Returned by Genesis Using Laser Post Ionization Secondary Neutral Mass Spectrometry

    Science.gov (United States)

    Veryovkin, I. V.; Calaway, W. F.; Tripa, C. E.; Pellin, M. J.; Burnett, D. S.

    2005-12-01

    A new secondary neutral mass spectrometry (SNMS) instrument implementing laser post ionization (LPI) of ion sputtered and laser desorbed neutral species has been developed and constructed for the specific purpose of quantitative analysis of metallic elements at ultra trace levels in solar wind collector samples returned to Earth by the Genesis Discovery mission. The first LPI SNMS measurements are focusing on determining Al, Ca, Cr, and Mg in these samples. These measurements provide the first concentration and isotopic abundances determinations for several key metallic elements and also elucidate possible fractionation effects between the photosphere and the solar wind compositions. It is now documented that Genesis samples suffered surface contamination both during flight and during the breach of the Sample Return Capsule when it crashed. Since accurate quantitative analysis is compromised by sample contamination, several features have been built into the new LPI SNMS instrument to mitigate this difficulty. A normally-incident, low-energy (SNMS instrument to further reduce quantification problems. The current status of instrument tests and analyses will be presented. This work is supported by the U. S. Department of Energy, BES-Materials Sciences, under Contract W-31-109-ENG-38, and by NASA under Work Orders W-19,895 and W-10,091.

  1. Finite element analysis of multi-piece post-crown restoration using different types of adhesives

    Institute of Scientific and Technical Information of China (English)

    Lin-Wei Lu; Guang-Wei Meng; Zhi-Hui Liu

    2013-01-01

    The multi-piece post-crown technique is more effective in restoring residual root than other restoration techniques. Various types of adhesives have different material properties that affect restoration. Therefore, the choice of adhesive is particularly important for patients. However, the effect of different kinds of adhesives was not too precise by experimental methods when concerning about individual differences of teeth. One tooth root can only be restored with one type of adhesive in experiment. After the mechanical test, this tooth root cannot be restored with other adhesives. With the help of medical imaging technology, reverse engineering and finite element analysis, a molar model can be reconstructed precisely and restored using different types of adhesives. The same occlusal and chewing loads were exerted on the same restored residual root models with different types of adhesives separately. Results of von Mises stress analysis showed that the adhesives with low Young’s modulus can protect the root canal effectively. However, a root canal concentration is apparently produced around the root canal orifice when chewing. Adhesives with large Young’s modulus can buffer the stress concentration of the root canal orifice. However, the root canal tissue may be destroyed because the adhesive is too hard to buffer the load.

  2. Software Analysis of Uncorrelated MS1 Peaks for Discovery of Post-Translational Modifications.

    Science.gov (United States)

    Pascal, Bruce D; West, Graham M; Scharager-Tapia, Catherina; Flefil, Ricardo; Moroni, Tina; Martinez-Acedo, Pablo; Griffin, Patrick R; Carvalloza, Anthony C

    2015-12-01

    The goal in proteomics to identify all peptides in a complex mixture has been largely addressed using various LC MS/MS approaches, such as data dependent acquisition, SRM/MRM, and data independent acquisition instrumentation. Despite these developments, many peptides remain unsequenced, often due to low abundance, poor fragmentation patterns, or data analysis difficulties. Many of the unidentified peptides exhibit strong evidence in high resolution MS(1) data and are frequently post-translationally modified, playing a significant role in biological processes. Proteomics Workbench (PWB) software was developed to automate the detection and visualization of all possible peptides in MS(1) data, reveal candidate peptides not initially identified, and build inclusion lists for subsequent MS(2) analysis to uncover new identifications. We used this software on existing data on the autophagy regulating kinase Ulk1 as a proof of concept for this method, as we had already manually identified a number of phosphorylation sites Dorsey, F. C. et al (J. Proteome. Res. 8(11), 5253-5263 (2009)). PWB found all previously identified sites of phosphorylation. The software has been made freely available at http://www.proteomicsworkbench.com . Graphical Abstract ᅟ. PMID:26265041

  3. Software Analysis of Uncorrelated MS1 Peaks for Discovery of Post-Translational Modifications

    Science.gov (United States)

    Pascal, Bruce D.; West, Graham M.; Scharager-Tapia, Catherina; Flefil, Ricardo; Moroni, Tina; Martinez-Acedo, Pablo; Griffin, Patrick R.; Carvalloza, Anthony C.

    2015-12-01

    The goal in proteomics to identify all peptides in a complex mixture has been largely addressed using various LC MS/MS approaches, such as data dependent acquisition, SRM/MRM, and data independent acquisition instrumentation. Despite these developments, many peptides remain unsequenced, often due to low abundance, poor fragmentation patterns, or data analysis difficulties. Many of the unidentified peptides exhibit strong evidence in high resolution MS1 data and are frequently post-translationally modified, playing a significant role in biological processes. Proteomics Workbench (PWB) software was developed to automate the detection and visualization of all possible peptides in MS1 data, reveal candidate peptides not initially identified, and build inclusion lists for subsequent MS2 analysis to uncover new identifications. We used this software on existing data on the autophagy regulating kinase Ulk1 as a proof of concept for this method, as we had already manually identified a number of phosphorylation sites Dorsey, F. C. et al (J. Proteome. Res. 8(11), 5253-5263 (2009)). PWB found all previously identified sites of phosphorylation. The software has been made freely available at http://www.proteomicsworkbench.com URL"/> .

  4. Common errors in mass spectrometry-based analysis of post-translational modifications.

    Science.gov (United States)

    Kim, Min-Sik; Zhong, Jun; Pandey, Akhilesh

    2016-03-01

    Mass spectrometry (MS) is a powerful tool to analyze complex mixtures of proteins in a high-throughput fashion. Proteome analysis has already become a routine task in biomedical research with the emergence of proteomics core facilities in most research institutions. Post-translational modifications (PTMs) represent a mechanism by which complex biological processes are orchestrated dynamically at the systems level. MS is rapidly becoming popular to discover new modifications and novel sites of known PTMs, revolutionizing the current understanding of diverse signaling pathways and biological processes. However, MS-based analysis of PTMs has its own caveats and pitfalls that can lead to erroneous conclusions. Here, we review the most common errors in MS-based PTM analyses with the goal of adopting strategies that maximize correct interpretation in the context of biological questions that are being addressed. Finally, we provide suggestions that should help mass spectrometrists, bioinformaticians and biologists to perform and interpret MS-based PTM analyses more accurately. PMID:26667783

  5. Construction, purification, and characterization of a chimeric TH1 antagonist

    Directory of Open Access Journals (Sweden)

    Javier-González Luís

    2006-05-01

    Full Text Available Abstract Background TH1 immune response antagonism is a desirable approach to mitigate some autoimmune and inflammatory reactions during the course of several diseases where IL-2 and IFN-γ are two central players. Therefore, the neutralization of both cytokines could provide beneficial effects in patients suffering from autoimmune or inflammatory illnesses. Results A chimeric antagonist that can antagonize the action of TH1 immunity mediators, IFN-γ and IL-2, was designed, engineered, expressed in E. coli, purified and evaluated for its in vitro biological activities. The TH1 antagonist molecule consists of the extracellular region for the human IFNγ receptor chain 1 fused by a four-aminoacid linker peptide to human 60 N-terminal aminoacid residues of IL-2. The corresponding gene fragments were isolated by RT-PCR and cloned in the pTPV-1 vector. E. coli (W3110 strain was transformed with this vector. The chimeric protein was expressed at high level as inclusion bodies. The protein was partially purified by pelleting and washing. It was then solubilized with strong denaturant and finally refolded by gel filtration. In vitro biological activity of chimera was demonstrated by inhibition of IFN-γ-dependent HLA-DR expression in Colo 205 cells, inhibition of IFN-γ antiproliferative effect on HEp-2 cells, and by a bidirectional effect in assays for IL-2 T-cell dependent proliferation: agonism in the absence versus inhibition in the presence of IL-2. Conclusion TH1 antagonist is a chimeric protein that inhibits the in vitro biological activities of human IFN-γ, and is a partial agonist/antagonist of human IL-2. With these attributes, the chimera has the potential to offer a new opportunity for the treatment of autoimmune and inflammatory diseases.

  6. Chimeric creatures in Greek mythology and reflections in science.

    Science.gov (United States)

    Bazopoulou-Kyrkanidou, E

    2001-04-15

    "The Chimaera" in Homer's Iliad, "was of divine stock, not of men, in the forepart a lion, in the hinder a serpent, and in the midst a goat, ellipsis Bellerophon slew her, trusting in the signs of the gods." In Hesiod's Theogony it is emphasized that "Chimaera ellipsis had three heads, one of a grim-eyed lion, another of a goat, and another of a snakeellipsis". In addition to this interspecies animal chimera, human/animal chimeras are referred to in Greek mythology, preeminent among them the Centaurs and the Minotaur. The Centaurs, as horse/men, first appear in Geometric and early Archaic art, but in the literature not until early in the fifth century B.C. The bullheaded-man Minotaur, who is not certainly attested in the literary evidence until circa 500 B.C., first appears in art about 650 B.C. Attempts, in the fourth century B.C. and thereafter, to rationalize their mythical appearance were in vain; their chimeric nature retained its fascinating and archetypal form over the centuries. Early in the 1980s, experimental sheep/goat chimeras were produced removing the reproductive barrier between these two animal species. Late in the 1990s, legal, political, ethical, and moral fights loomed over a patent bid on human/animal chimeras. Chimeric technology is recently developed; however, the concept of chimerism has existed in literary and artistic form in ancient mythology. This is yet another example where art and literature precede scientific research and development. PMID:11337752

  7. Literature analysis on Harry Potter series under the perspective of post-modernism%Literature analysis on Harry Potter series under the perspective of post-modernism

    Institute of Scientific and Technical Information of China (English)

    汪莉

    2011-01-01

    Harry Potter series books are not only the children's books that show clear good and evil forces and heroism, but also carrying strong postmodern features. This paper is to analyze the feature of post-modernism in Harry Potter series in terms of magical realism, postmodern feminism and pluralism and so on for the purpose of offering a different perspective of studying the books.

  8. Chimeric elk/mouse prion proteins in transgenic mice

    OpenAIRE

    Tamguney, G; Giles, K; Oehler, A.; Johnson, NL; DeArmond, SJ; Prusiner, SB

    2013-01-01

    Chronic wasting disease (CWD) of deer and elk is a highly communicable neurodegenerative disorder caused by prions. Investigations of CWD are hampered by slow bioassays in transgenic (Tg) mice. Towards the development of Tg mice that will be more susceptible to CWD prions, we created a series of chimeric elk/mouse transgenes that encode the N terminus of elk PrP (ElkPrP) up to residue Y168 and the C terminus of mouse PrP (MoPrP) beyond residue 169 (mouse numbering), designated Elk3M(SNIVVK). ...

  9. Brain structure in post-traumatic stress disorder: A voxel-based morphometry analysis.

    Science.gov (United States)

    Tan, Liwen; Zhang, Li; Qi, Rongfeng; Lu, Guangming; Li, Lingjiang; Liu, Jun; Li, Weihui

    2013-09-15

    This study compared the difference in brain structure in 12 mine disaster survivors with chronic post-traumatic stress disorder, 7 cases of improved post-traumatic stress disorder symptoms, and 14 controls who experienced the same mine disaster but did not suffer post-traumatic stress disorder, using the voxel-based morphometry method. The correlation between differences in brain structure and post-traumatic stress disorder symptoms was also investigated. Results showed that the gray matter volume was the highest in the trauma control group, followed by the symptoms-improved group, and the lowest in the chronic post-traumatic stress disorder group. Compared with the symptoms-improved group, the gray matter volume in the lingual gyrus of the right occipital lobe was reduced in the chronic post-traumatic stress disorder group. Compared with the trauma control group, the gray matter volume in the right middle occipital gyrus and left middle frontal gyrus was reduced in the symptoms-improved group. Compared with the trauma control group, the gray matter volume in the left superior parietal lobule and right superior frontal gyrus was reduced in the chronic post-traumatic stress disorder group. The gray matter volume in the left superior parietal lobule was significantly positively correlated with the State-Trait Anxiety Inventory subscale score in the symptoms-improved group and chronic post-traumatic stress disorder group (r = 0.477, P = 0.039). Our findings indicate that (1) chronic post-traumatic stress disorder patients have gray matter structural damage in the prefrontal lobe, occipital lobe, and parietal lobe, (2) after post-traumatic stress, the disorder symptoms are improved and gray matter structural damage is reduced, but cannot recover to the trauma-control level, and (3) the superior parietal lobule is possibly associated with chronic post-traumatic stress disorder. Post-traumatic stress disorder patients exhibit gray matter abnormalities. PMID:25206550

  10. Brain structure in post-traumatic stress disorder A voxel-based morphometry analysis**

    Institute of Scientific and Technical Information of China (English)

    Liwen Tan; Li Zhang; Rongfeng Qi; Guangming Lu; Lingjiang Li; Jun Liu; Weihui Li

    2013-01-01

    This study compared the difference in brain structure in 12 mine disaster survivors with chronic post-traumatic stress disorder, 7 cases of improved post-traumatic stress disorder symptoms, and 14 controls who experienced the same mine disaster but did not suffer post-traumatic stress disorder, us-ing the voxel-based morphometry method. The correlation between differences in brain structure and post-traumatic stress disorder symptoms was also investigated. Results showed that the gray matter volume was the highest in the trauma control group, fol owed by the symptoms-improved group, and the lowest in the chronic post-traumatic stress disorder group. Compared with the symptoms-improved group, the gray matter volume in the lingual gyrus of the right occipital lobe was reduced in the chronic post-traumatic stress disorder group. Compared with the trauma control group, the gray matter volume in the right middle occipital gyrus and left middle frontal gyrus was reduced in the symptoms-improved group. Compared with the trauma control group, the gray matter volume in the left superior parietal lobule and right superior frontal gyrus was reduced in the chronic post-traumatic stress disorder group. The gray matter volume in the left superior parietal lobule was significantly positively correlated with the State-Trait Anxiety Inventory subscale score in the symptoms-improved group and chronic post-traumatic stress disorder group (r = 0.477, P = 0.039). Our findings indicate that (1) chronic post-traumatic stress disorder patients have gray matter structural damage in the prefrontal lobe, occip-ital lobe, and parietal lobe, (2) after post-traumatic stress, the disorder symptoms are improved and gray matter structural damage is reduced, but cannot recover to the trauma-control level, and (3) the superior parietal lobule is possibly associated with chronic post-traumatic stress disorder. Post-traumatic stress disorder patients exhibit gray matter abnormalities.

  11. Role of C-terminal domain and transmembrane helices 5 and 6 in function and quaternary structure of major intrinsic proteins: analysis of aquaporin/glycerol facilitator chimeric proteins.

    Science.gov (United States)

    Duchesne, Laurence; Pellerin, Isabelle; Delamarche, Christian; Deschamps, Stephane; Lagree, Valerie; Froger, Alexandrine; Bonnec, Georgette; Thomas, Daniel; Hubert, Jean-Francois

    2002-06-01

    We previously observed that aquaporins and glycerol facilitators exhibit different oligomeric states when studied by sedimentation on density gradients following nondenaturing detergent solubilization. To determine the domains of major intrinsic protein (MIP) family proteins involved in oligomerization, we constructed protein chimeras corresponding to the aquaporin AQPcic substituted in the loop E (including the proximal part of transmembrane domain (TM) 5) and/or the C-terminal part (including the distal part of TM 6) by the equivalent domain of the glycerol channel aquaglyceroporin (GlpF) (chimeras called AGA, AAG, and AGG). The analogous chimeras of GlpF were also constructed (chimeras GAG, GGA, and GAA). cRNA corresponding to all constructs were injected into Xenopus oocytes. AQPcic, GlpF, AAG, AGG, and GAG were targeted to plasma membranes. Water or glycerol membrane permeability measurements demonstrated that only the AAG chimera exhibited a channel function corresponding to water transport. Analysis of all proteins expressed either in oocytes or in yeast by velocity sedimentation on sucrose gradients following solubilization by 2% n-octyl glucoside indicated that only AQPcic and AAG exist in tetrameric forms. GlpF, GAG, and GAA sediment in a monomeric form, whereas GGA and AGG were found mono/dimeric. These data bring new evidence that, within the MIP family, aquaporins and GlpFs behave differently toward nondenaturing detergents. We demonstrate that the C-terminal part of AQPcic, including the distal half of TM 6, can be substituted by the equivalent domain of GlpF (AAG chimera) without modifying the transport specificity. Our results also suggest that interactions of TM 5 of one monomer with TM 1 of the adjacent monomer are crucial for aquaporin tetramer stability. PMID:11927589

  12. Construction and characterization of chimeric BHIV (BIV/HIV-1) viruses carrying the bovine immunodeficiency virus gag gene

    OpenAIRE

    Zhu, Yi-Xin; Liu, Chang; Liu, Xin-Lei; Qiao, Wen-Tao; Chen, Qi-Min; Zeng, Yi; Geng, Yun-Qi

    2005-01-01

    AIM: To explore the possibility of the replacement of the gag gene between human immunodeficiency virus and bovine immunodeficiency virus, to achieve chimeric virions, and thereby gain a new kind of AIDS vaccine based on BHIV chimeric viruses.

  13. 3D-FE analysis of functionally graded structured dental posts.

    Science.gov (United States)

    Abu Kasim, Noor H; Madfa, Ahmed A; Hamdi, Mohd; Rahbari, Ghahnavyeh R

    2011-01-01

    This study aimed to compare the biomechanical behaviour of functionally graded structured posts (FGSPs) and homogenous-type posts in simulated models of a maxillary central incisor. Two models of FGSPs consisting of a multilayer xTi-yHA composite design, where zirconia and alumina was added as the first layer for models A and B respectively were compared to homogenous zirconia post (model C) and a titanium post (model D). The amount of Ti and HA in the FGSP models was varied in gradations. 3D-FEA was performed on all models and stress distributions were investigated along the dental post. In addition, interface stresses between the posts and their surrounding structures were investigated under vertical, oblique, and horizontal loadings. Strain distribution along the post-dentine interface was also investigated. The results showed that FGSPs models, A and B demonstrated better stress distribution than models C and D, indicating that dental posts with multilayered structure dissipate localized and interfacial stress and strain more efficiently than homogenous-type posts. PMID:22123011

  14. Understanding Impact and Implications of Data Standards on Post Disaster Risk Analysis

    Science.gov (United States)

    Stevenson, Robert

    2010-05-01

    Although the physical and humanitarian effects of a natural catastrophe are often bound to the locality of the event the financial impacts can have global effects. This is particularly prominent in the re/insurance community, where through a number of market mechanisms and re/insurance structures financial loss is mitigated amongst many companies across the globe. The level of risk a company wishes to retain, given an event, represents the level of risk decision makers deem acceptable. Catastrophe risk modelling tools aid the estimation of risk retention and transfer mechanisms, and increasingly the level of capital required to withstand a catastrophic event. These tools rely on appropriate representations hazard, exposure, vulnerability and insurance conditions that reflect the reality of risk. In addition, accurate estimation of loss potential in the aftermath of a catastrophic event equally relies on the data available to assess the scale of damages experienced and to provide views on the likely scale of loss. A coherent and focussed data and modelling strategy is required to ensure that the risk assessment made is as accurate as possible. A fundamental factor in determining the accuracy of catastrophe output, is the quality of data entered. It is of vital importance, therefore, to have an understanding of both the data used as well as the standard of this data, which will so powerfully impact upon the decision making process. This is perhaps best illustrated through the study of historical events, such as Hurricane Katrina and Ike. The extent of data variance in post disaster analysis clearly demonstrates issues of data discrepancies, vintage, resolution and uncertainty propagation, and reflects on the standard of the original data utilized for modelling purposes and decision making. Using experience gained from recent events, this paper will explore current data variabilities, and the impacts on effective loss estimation, both in relation to reinsurance

  15. Evaluation of the Edinburgh Post Natal Depression Scale using Rasch analysis

    Directory of Open Access Journals (Sweden)

    Tennant Alan

    2006-06-01

    Full Text Available Abstract Background The Edinburgh Postnatal Depression Scale (EPDS is a 10 item self-rating post-natal depression scale which has seen widespread use in epidemiological and clinical studies. Concern has been raised over the validity of the EPDS as a single summed scale, with suggestions that it measures two separate aspects, one of depressive feelings, the other of anxiety. Methods As part of a larger cross-sectional study conducted in Melbourne, Australia, a community sample (324 women, ranging in age from 18 to 44 years: mean = 32 yrs, SD = 4.6, was obtained by inviting primiparous women to participate voluntarily in this study. Data from the EPDS were fitted to the Rasch measurement model and tested for appropriate category ordering, for item bias through Differential Item Functioning (DIF analysis, and for unidimensionality through tests of the assumption of local independence. Results Rasch analysis of the data from the ten item scale initially demonstrated a lack of fit to the model with a significant Item-Trait Interaction total chi-square (chi Square = 82.8, df = 40; p Conclusion The results of this study suggest that EPDS, in its original 10 item form, is not a viable scale for the unidimensional measurement of depression. Rasch analysis suggests that a revised eight item version (EPDS-8 would provide a more psychometrically robust scale. The revised cut points of 7/8 and 9/10 for the EPDS-8 show high levels of agreement with the original case identification for the EPDS-10.

  16. Discovery of mitochondrial chimeric-gene associated with cytoplasmic male sterility of HL-rice

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The mitochondrial genome libraries of HL-type sterile line(A) and maintainer line(B) have been constructed.Mitochondrial gene, atp6, was used to screen libraries, due to the different Southern and Northern blot results between sterile and maintainer line. Sequencing analysis of positive clones proved that there were two copies of atp6 gene in sterile line and only one in maintainer line. One copy of atpt6 in sterile line was same to that in maintainer line; the other showed different flanking sequence from the 49th nucleotide downstream of the termination codon of atp6 gene. A new chimeric gene, orfH79, was found in the region. OrfH79 had homology to mitochondrial gene coxⅡ and orfl07, and was special to HL-sterile cytoplasm.``

  17. Authentic display of a cholera toxin epitope by chimeric type 1 fimbriae

    DEFF Research Database (Denmark)

    Stentebjerg-Olesen, Bodil; Pallesen, Lars; Jensen, Lars Bogø;

    1997-01-01

    inserted. Several of the chosen positions seemed amenable even for large foreign inserts; the chimeric proteins were exposed on the bacterial surface and the cholera toxin epitope was authentically displayed, i.e. it was recognized on bacteria by specific antiserum. Display of chimeric fimbriae was tested...

  18. Authentic display of a cholera toxin epitope by chimeric type 1 fimbriae

    DEFF Research Database (Denmark)

    Stentebjerg-Olesen, Bodil; Pallesen, Lars; Jensen, Lars Bogø; Christiansen, Gunnar; Klemm, Per

    inserted. Several of the chosen positions seemed amenable even for large foreign inserts; the chimeric proteins were exposed on the bacterial surface and the cholera toxin epitope was authentically displayed, i.e. it was recognized on bacteria by specific antiserum. Display of chimeric fimbriae was tested...

  19. DIVA vaccine properties of the live chimeric pestivirus strain CP7_E2gif

    DEFF Research Database (Denmark)

    von Rosen, Tanya; Rangelova, Desislava Yordanova; Nielsen, Jens;

    2014-01-01

    Live modified vaccines to protect against classical swine fever virus (CSFV), based on chimeric pestiviruses, have been developed to enable serological Differentiation of Infected from Vaccinated Animals (DIVA). In this context, the chimeric virus CP7_E2gif vaccine candidate is unique as it does...

  20. 78 FR 16505 - Prospective Grant of Exclusive License: Chimeric West Nile/Dengue Viruses

    Science.gov (United States)

    2013-03-15

    ...: Chimeric West Nile/Dengue Viruses AGENCY: Centers for Disease Control and Prevention (CDC), Department of... license, in the field of use of in vitro diagnostics for dengue virus infection, to practice the... Application 61/049,342, filed 4/30/2008, entitled ``Engineered, Chimeric West Nile/Dengue Viruses;''...

  1. High-resolution air quality simulation over Europe with the chemistry transport model CHIMERE

    Directory of Open Access Journals (Sweden)

    E. Terrenoire

    2015-01-01

    The results suggest that future work should focus on the development of national bottom-up emission inventories including a better account for semi-volatile organic compounds and their conversion to SOA, the improvement of the CHIMERE urban parameterization, the introduction into CHIMERE of the coarse nitrate chemistry and an advanced parameterization accounting for windblown dust emissions.

  2. The Construction of Chimeric T-Cell Receptor with Spacer Base of Modeling Study of VHH and MUC1 Interaction

    OpenAIRE

    Nazanin Pirooznia; Sadegh Hasannia; Majid Taghdir; Fatemeh Rahbarizadeh; Morteza Eskandani

    2011-01-01

    Adaptive cell immunotherapy with the use of chimeric receptors leads to the best and most specific response against tumors. Chimeric receptors consist of a signaling fragment, extracellular spacer, costimulating domain, and an antibody. Antibodies cause immunogenicity; therefore, VHH is a good replacement for ScFv in chimeric receptors. Since peptide sequences have an influence on chimeric receptors, the effect of peptide domains on each other's conformation were investigated. CD3Zeta, CD28, ...

  3. MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice

    OpenAIRE

    Wu, Limin; Li, Nainong; Zhang, Mingfeng; XUE, SHENG-LI; Cassady, Kaniel; Lin, Qing; Riggs, Arthur D; Zeng, Defu

    2015-01-01

    Induction of MHC- or HLA-matched mixed chimerism does not cause graft-versus-host disease (GVHD) in animal models or humans, but matched mixed chimerism cannot reverse autoimmunity. MHC-mismatched mixed chimerism is required for reversal of autoimmunity. Here, we report that, using a clinically applicable conditioning regimen consisting of cyclophosphamide, pentostatin, and antithymocyte globulin, MHC-mismatched mixed chimerism is established in experimental autoimmune encephalomyelitis (EAE)...

  4. Identifying Multimedia Production Competencies and Skills of Instructional Design and Technology Professionals: An Analysis of Recent Job Postings

    Science.gov (United States)

    Sugar, William; Hoard, Brent; Brown, Abbie; Daniels, Lee

    2012-01-01

    In an effort to document necessary multimedia production competencies of Instructional Design and Technology graduates, a recent analysis of over 7 months' worth of Instructional Design and Technology job advertisements (n = 615) were conducted. Specific job skills from these postings were categorized and analyzed. The data set includes three job…

  5. Analysis of Post-Fire Vegetation Recovery in the Mediterranean Basin using MODIS Derived Vegetation Indices

    Science.gov (United States)

    Hawtree, Daniel; San Miguel, Jesus; Sedano, Fernando; Kempeneers, Pieter

    2010-05-01

    The Mediterranean basin region is highly susceptible to wildfire, with approximately 60,000 individual fires and half a million ha of natural vegetation burnt per year. Of particular concern in this region is the impact of repeated wildfires on the ability of natural lands to return to a pre-fire state, and of the possibility of desertification of semi-arid areas. Given these concerns, understanding the temporal patterns of vegetation recovery is important for the management of environmental resources in the region. A valuable tool for evaluating these recovery patterns are vegetation indices derived from remote sensing data. Previous research on post-fire vegetation recovery conducted in this region has found significant variability in recovery times across different study sites. It is unclear what the primary variables are affecting the differences in the rates of recovery, and if any geographic patterns of behavior exist across the Mediterranean basin. This research has primarily been conducted using indices derived from Landsat imagery. However, no extensive analysis of vegetation regeneration for large regions has been published, and assessment of vegetation recovery on the basis of medium-spatial resolution imagery such as that of MODIS has not yet been analyzed. This study examines the temporal pattern of vegetation recovery in a number of fire sites in the Mediterranean basin, using data derived from MODIS 16 -day composite vegetation indices. The intent is to develop a more complete picture of the temporal sequence of vegetation recovery, and to evaluate what additional factors impact variations in the recovery sequence. In addition, this study evaluates the utility of using MODIS derived vegetation indices for regeneration studies, and compares the findings to earlier studies which rely on Landsat data. Wildfires occurring between the years 2000 and 2004 were considered as potential study sites for this research. Using the EFFIS dataset, all wildfires

  6. Detection of looseness degree of dental posts using natural frequency analysis.

    Science.gov (United States)

    Hsieh, Sung-Chih; Lin, Shu-Li; Huang, Yung-Kai; Chang, Wei-Jen; Yuan, Chia-Chun; Lo, Yi-June; Lee, Sheng-Yang; Huang, Haw-Ming

    2013-08-01

    This study examines the relationship between the retention force and natural frequency of dental posts cemented with zinc phosphate cement. Forty-two decoronated maxillary incisors were collected and embedded in resin blocks. The 7-mm post spaces were prepared for all test samples prior to cementing the 13-mm Para-Posts with zinc phosphate cement. The natural frequency values and retention forces of the zinc phosphate cement-cemented Para-Posts in various liquid/powder mixing ratios were measured. Thereafter, the natural frequency values of the zinc phosphate cement-cemented posts were continuously detected during ultrasonic vibration. Moreover, both the natural frequency values and the dislodgement forces in response to ultrasonic vibrations were measured for the pretreated post samples. Results showed that the natural frequency values of the posts significantly decreased from 8.8 ± 0.8 to 5.0 ± 0.5 kHz (P zinc phosphate cement-cemented dental posts. PMID:23722497

  7. Post-Test Analysis of a 10-Year Sodium Heat Pipe Life Test

    Science.gov (United States)

    Rosenfeld, John H.; Locci, Ivan E.; Sanzi, James L.; Hull, David R.; Geng, Steven M.

    2011-01-01

    High-temperature heat pipes are being evaluated for use in energy conversion applications such as fuel cells, gas turbine re-combustors, Stirling cycle heat sources; and with the resurgence of space nuclear power both as reactor heat removal elements and as radiator elements. Long operating life and reliable performance are critical requirements for these applications. Accordingly, long-term materials compatibility is being evaluated through the use of high-temperature life test heat pipes. Thermacore, Inc., has carried out a sodium heat pipe 10-year life test to establish long-term operating reliability. Sodium heat pipes have demonstrated favorable materials compatibility and heat transport characteristics at high operating temperatures in air over long time periods. A representative one-tenth segment Stirling Space Power Converter heat pipe with an Inconel 718 envelope and a stainless steel screen wick has operated for over 87,000 hr (10 years) at nearly 700 C. These life test results have demonstrated the potential for high-temperature heat pipes to serve as reliable energy conversion system components for power applications that require long operating lifetime with high reliability. Detailed design specifications, operating history, and post-test analysis of the heat pipe and sodium working fluid are described. Lessons learned and future life test plans are also discussed.

  8. Comparing pre- and post-copulatory mate competition using social network analysis in wild crickets

    Science.gov (United States)

    Fisher, David N.; Rodríguez-Muñoz, Rolando

    2016-01-01

    Sexual selection results from variation in success at multiple stages in the mating process, including competition before and after mating. The relationship between these forms of competition, such as whether they trade-off or reinforce one another, influences the role of sexual selection in evolution. However, the relationship between these 2 forms of competition is rarely quantified in the wild. We used video cameras to observe competition among male field crickets and their matings in the wild. We characterized pre- and post-copulatory competition as 2 networks of competing individuals. Social network analysis then allowed us to determine 1) the effectiveness of precopulatory competition for avoiding postcopulatory competition, 2) the potential for divergent mating strategies, and 3) whether increased postcopulatory competition reduces the apparent reproductive benefits of male promiscuity. We found 1) limited effectiveness of precopulatory competition for avoiding postcopulatory competition; 2) males do not specifically engage in only 1 type of competition; and 3) promiscuous individuals tend to mate with each other, which will tend to reduce variance in reproductive success in the population and highlights the trade-off inherent in mate guarding. Our results provide novel insights into the works of sexual competition in the wild. Furthermore, our study demonstrates the utility of using network analyses to study competitive interactions, even in species lacking obvious social structure. PMID:27174599

  9. Analysis of RNA from Alzheimer's Disease Post-mortem Brain Tissues.

    Science.gov (United States)

    Clement, Christian; Hill, James M; Dua, Prerna; Culicchia, Frank; Lukiw, Walter J

    2016-03-01

    Alzheimer's disease (AD) is a uniquely human, age-related central nervous system (CNS) disorder for which there is no adequate experimental model. While well over 100 transgenic murine models of AD (TgAD) have been developed that recapitulate many of the neuropathological features of AD, key pathological features of AD such as progressive neuronal atrophy, neuron cell loss, and neurofibrillary tangle (NFT) formation have not been observed in any TgAD model to date. To more completely analyze and understand the neuropathology, altered neuro-inflammatory and innate-immune signaling pathways, and the complex molecular-genetics and epigenetics of AD, it is therefore necessary to rigorously examine short post-mortem interval (PMI) human brain tissues to gain a deeper and more thorough insight into the neuropathological mechanisms that characterize the AD process. This perspective-methods paper will highlight some important recent findings on the utilization of short PMI tissues in sporadic (idiopathic; of unknown origin) AD research with focus on the extraction and quantification of RNA, and in particular microRNA (miRNA) and messenger RNA (mRNA) and analytical strategies, drawing on the authors' combined 125 years of laboratory experience into this investigative research area. We sincerely hope that new investigators in the field of "gene expression analysis in neurological disease" will benefit from the observations presented here and incorporate these recent findings and observations into their future experimental planning and design. PMID:25631714

  10. Investigation on the application of the isotopic correlation technique in post-irradiation analysis and safeguards

    International Nuclear Information System (INIS)

    An extensive analysis of irradiated fuel shows that the theoretical prediction of isotopic ratios for actinides in PWRs is very satisfactory. Comparisons of theoretical results with those from post-irradiation measurements demonstrate the capability of the used data and methods: all calculated values are within the range of the interlaboratory deviations. Sensitivities for a variety of isotopic ratios on reactor parameters as burn-up, enrichment, boron concentration, fuel-to-moderator-ratio, etc. have been calculated. On the basis of these sensitivity studies, a recipe has been worked out how to apply the isotopic correlation techniques to safeguarding fissile material. The capability of this recipe is demonstrated for realistic situations. The essential characteristic of the proposed method lies in the application of several (e.g. six) selectively sensitive (with respect to the reactor parameters) isotopic correlations. Theory is used as a verified tool in deciding on erroneous measurements, accidental or intentional fuel substitution or diversion. The proposed recipe is verified with simulated as well as with realistic, actually observed examples. (orig.)

  11. Analysis of post deposition processing for CdTe/CdS thin film solar cells

    Energy Technology Data Exchange (ETDEWEB)

    McCandless, B.E.; Birkmire, R.W. (Inst. of Energy Conversion, Univ. of Delaware, Newark, DE (United States))

    1991-12-01

    A post-deposition process for optimizing the efficiency of thin film CdTe/CdS solar cells deposited by physical vapor deposition has been developed and the effects of the individual process steps on the materials and device properties have been analyzed. A 400degC heat treatment with CdCl{sub 2} restructures the CdTe resulting in enhanced grain size and crystallographic reorientation. Structural and optical measurements indicate interdiffusion of sulfur and tellurium during the heat treatment resulting in formation of a CdS{sub x}Te{sub 1-x} layer with a narrower band gap than CdTe. Bifacial current-voltage and quantum efficiency analysis of the CdTe devices at various stages of the optimization process shows the evolution of the device from a p-i-n structure to a heterojunction. A chemical treatment improves the open circuit voltage (V{sub oc}) and Cu/Au contact to the CdTe. The optimization process can be applied to cells using CdTe and CdS deposited by different methods. (orig.).

  12. A targeted change-detection procedure by combining change vector analysis and post-classification approach

    Science.gov (United States)

    Ye, Su; Chen, Dongmei; Yu, Jie

    2016-04-01

    In remote sensing, conventional supervised change-detection methods usually require effective training data for multiple change types. This paper introduces a more flexible and efficient procedure that seeks to identify only the changes that users are interested in, here after referred to as "targeted change detection". Based on a one-class classifier "Support Vector Domain Description (SVDD)", a novel algorithm named "Three-layer SVDD Fusion (TLSF)" is developed specially for targeted change detection. The proposed algorithm combines one-class classification generated from change vector maps, as well as before- and after-change images in order to get a more reliable detecting result. In addition, this paper introduces a detailed workflow for implementing this algorithm. This workflow has been applied to two case studies with different practical monitoring objectives: urban expansion and forest fire assessment. The experiment results of these two case studies show that the overall accuracy of our proposed algorithm is superior (Kappa statistics are 86.3% and 87.8% for Case 1 and 2, respectively), compared to applying SVDD to change vector analysis and post-classification comparison.

  13. In Silico Analysis of Correlations between Protein Disorder and Post-Translational Modifications in Algae

    Directory of Open Access Journals (Sweden)

    Atsushi Kurotani

    2015-08-01

    Full Text Available Recent proteome analyses have reported that intrinsically disordered regions (IDRs of proteins play important roles in biological processes. In higher plants whose genomes have been sequenced, the correlation between IDRs and post-translational modifications (PTMs has been reported. The genomes of various eukaryotic algae as common ancestors of plants have also been sequenced. However, no analysis of the relationship to protein properties such as structure and PTMs in algae has been reported. Here, we describe correlations between IDR content and the number of PTM sites for phosphorylation, glycosylation, and ubiquitination, and between IDR content and regions rich in proline, glutamic acid, serine, and threonine (PEST and transmembrane helices in the sequences of 20 algae proteomes. Phosphorylation, O-glycosylation, ubiquitination, and PEST preferentially occurred in disordered regions. In contrast, transmembrane helices were favored in ordered regions. N-glycosylation tended to occur in ordered regions in most of the studied algae; however, it correlated positively with disordered protein content in diatoms. Additionally, we observed that disordered protein content and the number of PTM sites were significantly increased in the species-specific protein clusters compared to common protein clusters among the algae. Moreover, there were specific relationships between IDRs and PTMs among the algae from different groups.

  14. Post-hoc Analysis on the R&D Capabilities of Chemical and Metallurgical Manufacturing

    Directory of Open Access Journals (Sweden)

    Herman Shah Anuar

    2013-09-01

    Full Text Available The purpose of this paper is to evaluate how internal R&D, external R&D, and patenting affects the behavior of foreign, local, and joint-venture companies operating in manufacturing companies in Malaysia. Different types of manufacturing companies may have different approach in applying their R&D capabilities and patenting activity. The construct of this paper is based on the post-hoc analysis in evaluating how internal R&D, external R&D, and patenting affects the behavior of foreign, local, and joint-venture companies operating in manufacturing companies. This research was conducted using survey questionnaires. 124 companies in chemical and metallurgical manufacturing companies participated in this survey. It was indicated that these three companies behave differently when dealing with internal R&D, external R&D, and patenting. It can be concluded that these three types of companies have a different perspective on applying internal R&D, external R&D, and patenting which is based on their different business strategic direction. It is suggested that in the near future, researchers should concentrate and other types of manufacturing companies or they can involve more sample size in getting better generalization on the behavior of these companies.

  15. Post-experimental analysis of a solid oxide fuel cell stack using hybrid seals

    Science.gov (United States)

    Thomann, O.; Rautanen, M.; Himanen, O.; Tallgren, J.; Kiviaho, J.

    2015-01-01

    A post-experimental analysis of a SOFC stack is presented. The stack was operated for 1800 h at 700 °C with air and hydrogen and contained hybrid glass-Thermiculite 866 seals. The goal of this work was to investigate the sealing microstructure and possible corrosion during mid-term operation. It was found that hybrid seals could effectively compensate for manufacturing tolerances of cells and other components due to the compliance of the glass layer. Additionally, different interfaces were investigated for corrosion. Corrosion was not observed at two-phase interfaces such as Crofer 22 APU/glass, glass/electrolyte and glass/Thermiculite 866. The three-phase interface between Crofer 22 APU/glass/hydrogen exhibited no corrosion. Some evidence of non-systematic corrosion was found at the Crofer 22 APU/glass/air interface. The possible reasons for the corrosion are discussed. Lastly, dual exposure to humid hydrogen and air of the 0.2 mm Crofer 22 APU interconnect had no detrimental effect on the corrosion compared to air exposure. Overall the hybrid seals used in combination with the thin interconnects were found to be a promising solution due to the low leak rate and limited material interactions.

  16. Acid Etching and Surface Coating of Glass-Fiber Posts: Bond Strength and Interface Analysis.

    Science.gov (United States)

    Cecchin, Doglas; Farina, Ana Paula; Vitti, Rafael Pino; Moraes, Rafael Ratto; Bacchi, Ataís; Spazzin, Aloísio Oro

    2016-01-01

    The aim of this study was to evaluate the bond strength of a composite resin to glass-fiber post (GFP) treated or not with phosphoric acid, silane coupling agent, and unfilled resin. GFPs were etched or not with 37% phosphoric acid and different surface coating applied: silane coupling agent, unfilled resin, or both. Composite resin blocks were built around a 4-mm height on the GFP. Unfilled resin (20 s) and composite resin (40 s) were light activated by a light-emitting diode unit. The specimens were stored in distilled water at 37 °C for 24 h. Microtensile bond test was performed using a mechanical testing machine until failure (n=10). The data were analyzed using two-way ANOVA followed by Student-Newman-Keuls' test (padhesive, mixed, or cohesive failures. Additional specimens (n=3) were made to analyze the bonded interfaces by scanning electron microscopy. The statistical analysis showed the factor 'surface coating' was significant (pbond strength was found for the silane and unfilled resin group (padhesive and cohesive failures was found. Differences regarding the homogeneity and thickness of the unfilled resin layer formed by different GFP surface treatments were observed. The application of silane and unfilled resin can improve the bond strength between GFP and resin composite. PMID:27058389

  17. Post-test analysis of lithium-ion battery materials at Argonne National Laboratory

    Science.gov (United States)

    Bareno, Javier; Dietz-Rago, Nancy; Bloom, Ira

    2014-03-01

    Electrochemical performance is often limited by surface and interfacial reactions at the electrodes. However, routine handling of samples can alter the very surfaces that are the object of study. Our approach combines standardized testing of batteries with sample harvesting under inert atmosphere conditions. Cells of different formats are disassembled inside an Argon glove box with controlled water and oxygen concentrations below 2 ppm. Cell components are characterized in situ, guaranteeing that observed changes in physicochemical state are due to electrochemical operation, rather than sample manipulation. We employ a complementary set of spectroscopic, microscopic, electrochemical and metallographic characterization to obtain a complete picture of cell degradation mechanisms. The resulting information about observed degradation mechanisms is provided to materials developers, both academic and industrial, to suggest new strategies and speed up the Research & Development cycle of Li-ion and related technologies. This talk will describe Argonne's post-test analysis laboratory, with an emphasis on capabilities and opportunities for collaboration. Cell disassembly, sample harvesting procedures and recent results will be discussed. This work was performed under the auspices of the U.S. Department of Energy, Office of Vehicle Technologies, Hybrid and Electric Systems, under Contract No. DE-AC02-06CH11357.

  18. Stress generated by customized glass fiber posts and other types by photoelastic analysis.

    Science.gov (United States)

    Bosso, Kátia; Gonini Júnior, Alcides; Guiraldo, Ricardo Danil; Berger, Sandrine Bittencourt; Lopes, Murilo Baena

    2015-01-01

    Endodontic posts are necessary to provide adequate retention and support when no sufficient remaining structure is available to retain the core. There are different materials and techniques to construct post-and-core, but there is no consensus about which one promotes better stress distribution on the remaining tooth structure. This study aimed to quantify and evaluate the distribution of stress in the root produced by customized glass fiber posts compared to different endodontic posts. Twenty-five simulated roots from photoelastic resin were made and divided into 5 groups: CPC, cast post-and-core; SP, screw post; CF, carbon fiber post; GF, glass fiber post; and CGF, customized glass fiber post. After cementing CPC and SP posts with zinc phosphate cement, and CF, GF and CGF posts with resin cement, resin cores were made for groups 2-5. Specimens were evaluated with vertical or 45° oblique loading. To analyze the fringes, the root was divided into 6 parts: palatal cervical, palatal middle, palatal apical, vestibular cervical, vestibular middle, and vestibular apical. The formed fringes were photographed and quantified. Data were recorded and subjected to two-way ANOVA and Tukey's test (5%). SP (1.95±0.60) showed higher stress (p<0.05) compared to the others (CPC-0.52±0.74; CF-0.50±0.75, GF-0.23±0.48 and CGF-0.45±0.83). All posts showed high stress in apical third (CPC-1.40±0.65; SP-2.30±0.44, CF-1.80±0.45, GF-1.20±0.45, CGF-1.70±1.03) Low stress was found in cervical third (CPC-0.20±0.45; CF-0.00±0.00, GF-0.00±0.00, CGF-0.00±0.00), except by SP (1.90±0.65), which showed statistical difference (p<0.05). Customized post showed high stress concentration at the root and conventional glass fiber posts showed more favorable biomechanical behavior. PMID:26200144

  19. 78 FR 13691 - Prospective Grant of Exclusive License: The Development of m971 and m972 Chimeric Antigen...

    Science.gov (United States)

    2013-02-28

    ... m971 and m972 Chimeric Antigen Receptors (CARs) for the Treatment of B Cell Malignancies AGENCY... inventions embodied in (a) U.S. Patent Application 61/717,960 entitled ``M971 Chimeric Antigen Receptors... their cell surface using chimeric antigen receptors which contain the m971 or m972 antibody...

  20. Serotype Chimeric Human Adenoviruses for Cancer GeneTherapy

    Directory of Open Access Journals (Sweden)

    Akseli Hemminki

    2010-09-01

    Full Text Available Cancer gene therapy consists of numerous approaches where the common denominator is utilization of vectors for achieving therapeutic effect. A particularly potent embodiment of the approach is virotherapy, in which the replication potential of an oncolytic virus is directed towards tumor cells to cause lysis, while normal cells are spared. Importantly, the therapeutic effect of the initial viral load is amplified through viral replication cycles and production of progeny virions. All cancer gene therapy approaches rely on a sufficient level of delivery of the anticancer agent into target cells. Thus,enhancement of delivery to target cells, and reduction of delivery to non-target cells, in an approach called transductional targeting, is attractive. Both genetic and non-genetic retargeting strategies have been utilized. However, in the context of oncolytic viruses, it is beneficial to have the specific modification included in progeny virions and hence genetic modification may be preferable. Serotype chimerism utilizes serotype specific differences in receptor usage, liver tropism and seroprevalence in order to gain enhanced infection of target tissue. This review will focus on serotype chimeric adenoviruses for cancer gene therapy applications.

  1. Pathology Tissue-quantitative Mass Spectrometry Analysis to Profile Histone Post-translational Modification Patterns in Patient Samples*

    OpenAIRE

    Noberini, Roberta; Uggetti, Andrea; Pruneri, Giancarlo; Minucci, Saverio; Bonaldi, Tiziana

    2015-01-01

    Histone post-translational modifications (hPTMs) generate a complex combinatorial code that has been implicated with various pathologies, including cancer. Dissecting such a code in physiological and diseased states may be exploited for epigenetic biomarker discovery, but hPTM analysis in clinical samples has been hindered by technical limitations. Here, we developed a method (PAThology tissue analysis of Histones by Mass Spectrometry - PAT-H-MS) that allows to perform a comprehensive, unbias...

  2. Hydraulic analysis of river training cross-vanes as part of post-restoration monitoring

    Directory of Open Access Journals (Sweden)

    T. A. Endreny

    2011-07-01

    Full Text Available River restoration design methods are incrementally improved by studying and learning from monitoring data in previous projects. In this paper we report post-restoration monitoring data and simulation analysis for a Natural Channel Design (NCD restoration project along 1600 m of the Batavia Kill (14 km2 watershed in the Catskill Mountains, NY. The restoration project was completed in 2002 with goals to reduce bank erosion and determine the efficacy of NCD approaches for restoring headwater streams in the Catskill Mountains, NY. The NCD approach used a reference-reach to determine channel form, empirical relations between the project site and reference site bankfull dimensions to size channel geometry, and hydraulic and sediment computations based on a bankfull (1.3 yr return interval discharge to test channel capacity and sediment stability. The NCD project included 12 cross-vanes and 48 j-hook vanes as river training structures along 19 meander bends to protect against bank erosion and maintain scour pools for fish habitat. Monitoring data collected from 2002 to 2004 were used to identify aggradation of pools in meander bends and below some structures. Aggradation in pools was attributed to the meandering riffle-pool channel trending toward step-pool morphology and cross-vane arms not concentrating flow in the center of the channel. The aggradation subsequently caused flow splitting and 4 partial point bar avulsions during a spring 2005 flood with a 25-yr return interval. Processing the pre-flood monitoring data with hydraulic analysis software provided clues the reach was unstable and preventative maintenance was needed. River restoration and monitoring teams should be trained in robust hydraulic analytical methods that help them extend project restoration goals and structure stability.

  3. The aetiology of post-traumatic stress following childbirth: a meta-analysis and theoretical framework.

    Science.gov (United States)

    Ayers, S; Bond, R; Bertullies, S; Wijma, K

    2016-04-01

    There is evidence that 3.17% of women report post-traumatic stress disorder (PTSD) after childbirth. This meta-analysis synthesizes research on vulnerability and risk factors for birth-related PTSD and refines a diathesis-stress model of its aetiology. Systematic searches were carried out on PsycINFO, PubMed, Scopus and Web of Science using PTSD terms crossed with childbirth terms. Studies were included if they reported primary research that examined factors associated with birth-related PTSD measured at least 1 month after birth. In all, 50 studies (n = 21 429) from 15 countries fulfilled inclusion criteria. Pre-birth vulnerability factors most strongly associated with PTSD were depression in pregnancy (r = 0.51), fear of childbirth (r = 0.41), poor health or complications in pregnancy (r = 0.38), and a history of PTSD (r = 0.39) and counselling for pregnancy or birth (r = 0.32). Risk factors in birth most strongly associated with PTSD were negative subjective birth experiences (r = 0.59), having an operative birth (assisted vaginal or caesarean, r = 0.48), lack of support (r = -0.38) and dissociation (r = 0.32). After birth, PTSD was associated with poor coping and stress (r = 0.30), and was highly co-morbid with depression (r = 0.60). Moderator analyses showed that the effect of poor health or complications in pregnancy was more apparent in high-risk samples. The results of this meta-analysis are used to update a diathesis-stress model of the aetiology of postpartum PTSD and can be used to inform screening, prevention and intervention in maternity care. PMID:26878223

  4. Reproducible Analysis of Post-Translational Modifications in Proteomes--Application to Human Mutations.

    Directory of Open Access Journals (Sweden)

    Alex S Holehouse

    Full Text Available Protein post-translational modifications (PTMs are an important aspect of protein regulation. The number of PTMs discovered within the human proteome, and other proteomes, has been rapidly expanding in recent years. As a consequence of the rate in which new PTMs are identified, analysis done in one year may result in different conclusions when repeated in subsequent years. Among the various functional questions pertaining to PTMs, one important relationship to address is the interplay between modifications and mutations. Specifically, because the linear sequence surrounding a modification site often determines molecular recognition, it is hypothesized that mutations near sites of PTMs may be more likely to result in a detrimental effect on protein function, resulting in the development of disease.We wrote an application programming interface (API to make analysis of ProteomeScout, a comprehensive database of PTMs and protein information, easy and reproducible. We used this API to analyze the relationship between PTMs and human mutations associated with disease (based on the 'Clinical Significance' annotation from dbSNP. Proteins containing pathogenic mutations demonstrated a significant study bias which was controlled for by analyzing only well-studied proteins, based on their having at least one pathogenic mutation. We found that pathogenic mutations are significantly more likely to lie within eight amino acids of a phosphoserine, phosphotyrosine or ubiquitination site when compared to mutations in general, based on a Fisher's Exact test. Despite the skew of pathogenic mutations occurring on positively charged arginines, we could not account for this relationship based only on residue type. Finally, we hypothesize a potential mechanism for a pathogenic mutation on RAF1, based on its proximity to a phosphorylation site, which represents a subtle regulation difference that may explain why its biochemical effect has failed to be uncovered

  5. Post Tyrrhénian deformation analysis in the Sahel coast (Eastern Tunisia): seismotectonic events implication

    Science.gov (United States)

    Mejrei, H.; Ghribi, R.; Bouaziz, S.; Balescu, S.

    2012-04-01

    The eastern coast of Tunisia is characterized by Pleistocene coastal deposits considered as a reference of interglacial high sea levels. In this region, the stratigraphy of Tunisian Pleistocene deposits was first established on the basis of geomorphological, lithostratigraphic, biostratigraphic criteria and U/Th data. They have been subdivided into three superimposed formations, from the oldest to the recent "Douira, Rejiche and Chebba" including coastal marine (Strombus bubonius), lagoonal and eolian sediments. These marine formations are organized into parallel bars to the actual shoreline overlaying unconformably the Mio-Pliocene and "Villafranchian" deposits. A luminescence dating method IRSL applied to alkali feldspar grains from the two sandy marines units of the Douira formation demonstrate for the first time the presence of two successive interglacial high sea level events correlative of MIS 7 and MIS 9. These sandy marine units are separated by a major erosional surface and by a continental pedogenised loamy deposit related to a low sea level event which might be assigned to MIS 8. Variations in the height of these marine unit (+13 to +32m) in the Sahel coast reflect a significant tectonic deformations and show precious geomorphological and tectonic markers. An extensive brittle deformations analysis has been carried out in several sites. A detailed analysis of fracturing is based on studies of fault-slip data population and of joint sets. It allows reconstructions of post Tyrrhenian stress regimes which are characterized by N170-016 compression and N095-100 extension. In this paper we present, the combination of IRSL data applied to these raised marine deposits and a reconstruction of tectonic evolution in term of stress pattern evolution since the Tyrrhenian allowed us to assign an accurate the recent tectonic calendar. These reconstituted events will be replaced and will be discussed in the regional setting of sismotectonic activities of the north

  6. Post test analysis of ROSA-III double-ended break test RUN 901

    International Nuclear Information System (INIS)

    The ROSA-III test facility is a volumetrically scaled (1/424) boiling water reactor (BWR) system with an electrically heated core. The purpose of ROSA-III experiment is to study the thermal-hydraulic behavior and the performance of the emergency core cooling system (ECCS) during a postulated loss-of-coolant accident (LOCA) and to provide the data base for the assessment and improvement of reactor safety analysis codes. RUN 901 was a first ROSA-III experiment with the fourth fuel assembly and assumed a 200 % double-ended break at the recirculation pump section line with full ECCS actuation. Post test analyses of RUN 901 were performed with the computer codes RELAP4J and RELAP5/MOD1/001. The system pressure response calculated with the two codes agreed well with the data. The agreement was also good for the core inlet flow behavior until the beginning of the lower plenum flashing (LPF). RELAP4J is a fast running code and calculated well the overall behavior of the mixture level in the core. However, the spray water was accumulated in the upper plenum (UP) due to the inability of the code to calculate counter current flow at the upper tie plate (UTP). While spray water was not accumulated in the upper plenum in the experiment. RELAP5 with an advanced two-phase flow model calculated well the rewetting of the fuel after the LPF and the top-down quench after the uncovery of the whole core. However, the incorporation of a CCFL model and/or the improvement in the interphase drag correlations are necessary to be able to calculate the mixture level behavior more accurately. An appropriate discharge coefficient is also necessary to calculate the break flow accurately with the RELAP5 characteristic analysis break flow model. (author)

  7. Struggles for recognition: a content analysis of messages posted on the Internet

    Directory of Open Access Journals (Sweden)

    Andersen AJ

    2012-07-01

    Full Text Available Anders Johan W Andersen,1,2 Tommy Svensson31Department of Psychosocial Health, University of Agder, Norway; 2Nordic School of Public Health, Gothenburg, Sweden; 3Department of Behavioral Sciences and Learning, Linköping University, Linköping, SwedenBackground: The Internet has enlarged the possibilities of human communication and opened new ways of exploring perceptions of mental health. This study is part of a research project aiming to explore, describe, and analyze different discourses of mental health in Norway and Sweden, using material from Internet-based services.Aim: To examine messages posed by users of publicly available question-and-answer services and to describe their content.Methods: A Web search was used to identify Norwegian and Swedish Websites offering mental health services by email or posted messages. A total of 601 messages from 20 services, 10 Norwegian and 10 Swedish, were analyzed by means of qualitative content analysis and further interpreted in light of the social theory of recognition by Honneth.Results: Eight categories emerged from the analysis: family life, couples, others, violence, the ungovernable, self-image, negotiating normality, and life struggles. These categories were then grouped into three themes: (1 relationship to significant others, (2 relationship to self, and (3 relationship to the social community. The themes promoted an understanding of mental health as closely connected to political and social factors.Conclusions: The results showed a variety of concerns from various parts of life and empowered the view that mental health should be understood broadly, at a conceptual level. Mental health emerged as a deeply relational concept that emphasized the equal distribution of chances in life. It strengthened the moral grammar of social inclusion and the acceptance of plurality in social life.Keywords: Internet-based services, mental health, public health, social theory

  8. Post-Messinian evolution of the Florence Ridge area (Western Cyprus Arc), Part I: Morphostructural analysis

    Science.gov (United States)

    Sellier, N. C.; Loncke, L.; Vendeville, B. C.; Mascle, J.; Zitter, T.; Woodside, J.; Loubrieu, B.

    2013-04-01

    The Florence Ridge, part of the western Cyprus arc, is a compressional relief that was eroded during the Messinian salinity crisis while deposition of salt occurred North (Antalya basin) and South (Herodotus abyssal plain). In order to better assess the impact of salt-tectonics in the Florence Ridge region deformations, we conducted a morpho-structural analysis of available multibeam and seismic data (Simed and Prismed II campaigns). It is indeed a crucial issue to distinguish crustal and gravity driven structures in the compressional to strike-slip belts of the eastern Mediterranean. Along the Antalya basin, we mainly observed multi-directional tectonic rafts typical of gravity gliding above salt. On the Florence Ridge itself, the base of salt evolves laterally to a Messinian erosional surface that erodes a series of stacked nappes. This surface is involved in recent faulting. South of the Florence Ridge, a nearly 100 km wide fold belt characterizes the Herodotus abyssal plain. Three different zones parallel to the Florence Ridge appear within this fold belt. Those are respectively from North to South zones A, B and C. Zone A is characterized mainly by small-wavelength folding and faulting. Approaching Zone B, a long extensional graben deforms the seafloor. Zone B stands ~ 100 to 200 m higher than zones A and C. There, salt welding seems common. In the easternmost zone B deep sub-circular bathymetric depressions are associated with extremely thick and fan-shaped depocenters probably emplaced in relation with active sub-salt thrusts. Many evidences suggest post-Messinian uplift in this zone. Zone C shows medium to high wavelength salt-cored folds. Wavelength of those folds increase approaching the distal Nile deep-sea fan. Within zone C, a nearly undeformed domain exists approaching the Eratosthenes seamount. 'En echelon' folds bound this flat domain suggesting lateral salt extrusion at the junction between zone C and the distal Nile deep-sea fan. To conclude

  9. Laser Post-Ionization Mass Spectrometry Analysis of Genesis Solar Wind Collectors

    Science.gov (United States)

    Veryovkin, I. V.; Tripa, C. E.; Zinovev, A. V.; Hiller, J. M.; Pellin, M. J.; Burnett, D. S.

    2008-12-01

    The samples returned to Earth by the NASA's Genesis Mission contain a record of the elemental and isotopic abundances of the Solar Wind (SW). This record is formed by the SW ions implanted in the near-surface regions of the Genesis sample collectors, so that the SW material can be distinguished from a terrestrial contamination, which occurred due to the crash landing of the spacecraft Sample Return Capsule. At Argonne National Laboratory, we are conducting analyzes of the Genesis SW collectors using a specially developed Laser Post-Ionization Secondary Neutral Mass Spectrometer (LPI SNMS), SARISA. This approach, based on ion sputtering of a SW collector surface and laser post-ionization of the neutral atoms sputtered from it, has proved to be sensitive, accurate and well suited for the quantitative analysis of the Genesis samples. We will report in this work the abundances of SW Mg and Ca measured with SARISA in two types of SW collector materials, silicon and diamond-like carbon (DLC). These LPI SNMS measurements were conducted in Resonance-Enhanced Multi-Photon Ionization (REMPI) regime using a sputter depth profiling method. In order to make our analyzes quantitative, we used specially prepared standards, made from exactly the same materials as the flown Genesis SW collectors and implanted with known fluencies of Mg and Ca ions. The REMPI analyzes of these standards allowed us to characterize the actual efficiency and detection limits of the SARISA instrument: for Mg, its useful yield peaked at about 20% and detection limits corresponded to SNMS measurements, we conducted a special series of experiments with sputter depth profiling of Genesis SW collectors from their backside (i.e. opposite to the one exposed to the SW). By these measurements, we have demonstrated that (1) the implanted solar wind can be identified by backside sputter depth profiling, (2) the apparent widening of depth profiles into the bulk of SW collectors is mostly due to ion mixing effects

  10. Analysis of Pre- and Post-Monsoon Suspended Sediments in the Gulf of Kachchh, India Using Remote Sensing

    OpenAIRE

    Gupta, Mukesh

    2015-01-01

    A comprehensive study of satellite-derived suspended sediment concentration (SSC) during pre- and post-monsoon has been conducted with full-month cycles of tidal responses to study the suspended sediment dynamics in the Gulf of Kachchh. Tidal data were interpreted in conjunction with the OCEANSAT-1 ocean color monitor (OCM)-derived SSC for pre- and post-monsoon. The analysis of the data shows that the Gulf is predominantly affected by the tidal changes. The average SSC during pre-monsoon were...

  11. Quantification of energy savings from Ireland's Home Energy Saving scheme. An ex post billing analysis

    Energy Technology Data Exchange (ETDEWEB)

    Scheer, J.; Clancy, M.; Ni Hogain, S. [Sustainable Energy Authority of Ireland, Wilton Park House, Wilton Terrace, Dublin 2 (Ireland)

    2013-02-15

    This paper quantifies the energy savings realised by a sample of participants in the Sustainable Energy Authority of Ireland's Home Energy Saving (HES) residential retrofit scheme (currently branded as the Better Energy Homes scheme), through an ex post billing analysis. The billing data are used to evaluate: (1) the reduction in gas consumption of the sample between pre- (2008) and post- (2010) scheme participation when compared to the gas consumption of a control group, (2) an estimate of the shortfall when this result is compared to engineering-type ex ante savings estimates and (3) the degree to which these results may apply to the wider population. All dwellings in the study underwent energy efficiency improvements, including insulation upgrades (wall and/or roof), installation of high-efficiency boilers and/or improved heating controls, as part of the HES scheme. Metered gas use data for the 210 households were obtained from meter operators for a number of years preceding dwelling upgrades and for a post-intervention period of 1 year. Dwelling characteristics and some household behavioural data were obtained through a survey of the sample. The gas network operator provided anonymised data on gas usage for 640,000 customers collected over the same period as the HES sample. Dwelling type data provided with the population dataset enabled matching with the HES sample to increase the internal validity of the comparison between the control (matched population data) and the treatment (HES sample). Using a difference-in-difference methodology, the change in demand of the sample was compared with that of the matched population subset of gas-using customers in Ireland over the same time period. The mean reduction in gas demand as a result of energy efficiency upgrades for the HES sample is estimated as 21 % or 3,664{+-}603 kWh between 2008 and 2010. An ex ante estimate of average energy savings, based on engineering calculations (u value reductions and improved boiler

  12. Sex Differences in Hookah-Related Images Posted on Tumblr: A Content Analysis.

    Science.gov (United States)

    Primack, Brian A; Carroll, Mary V; Shensa, Ariel; Davis, Wesley; Levine, Michele D

    2016-01-01

    Hookah tobacco smoking is prevalent, widespread, and associated with large amounts of toxicants. Hookah tobacco smoking may be viewed differently by males and females. For example, females have been drawn to types of tobacco that are flavored, milder, and marketed as more social and exotic. Individuals often use the growing segment of anonymous social networking sites, such as Tumblr, to learn about potentially dangerous or harmful behaviors. We used a systematic process involving stratification by time of day, day of week, and search term to gather a sample of 140 Tumblr posts related to hookah tobacco smoking. After a structured codebook development process, 2 coders independently assessed all posts in their entirety, and all disagreements were easily adjudicated. When data on poster sex and age were available, we found that 77% of posts were posted by females and 35% were posted by individuals younger than 18. The most prominent features displayed in all posts were references to or images of hookahs themselves, sexuality, socializing, alcohol, hookah smoke, and tricks performed with hookah smoke. Compared with females, males more frequently posted images of hookahs and alcohol-related images or references. This information may help guide future research in this area and the development of targeted interventions to curb this behavior. PMID:26890733

  13. A Human Reliability Analysis of Post- Accident Human Errors in the Low Power and Shutdown PSA of KSNP

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Daeil; Kim, J. H.; Jang, S. C

    2007-03-15

    Korea Atomic Energy Research Institute, using the ANS low power and shutdown (LPSD) probabilistic risk assessment (PRA) Standard, evaluated the LPSD PSA model of the KSNP, Yonggwang Units 5 and 6, and identified the items to be improved. The evaluation results of human reliability analysis (HRA) of the post-accident human errors in the LPSD PSA model for the KSNP showed that 10 items among 19 items of supporting requirements for those in the ANS PRA Standard were identified as them to be improved. Thus, we newly carried out a HRA for post-accident human errors in the LPSD PSA model for the KSNP. Following tasks are the improvements in the HRA of post-accident human errors of the LPSD PSA model for the KSNP compared with the previous one: Interviews with operators in the interpretation of the procedure, modeling of operator actions, and the quantification results of human errors, site visit. Applications of limiting value to the combined post-accident human errors. Documentation of information of all the input and bases for the detailed quantifications and the dependency analysis using the quantification sheets The assessment results for the new HRA results of post-accident human errors using the ANS LPSD PRA Standard show that above 80% items of its supporting requirements for post-accident human errors were graded as its Category II. The number of the re-estimated human errors using the LPSD Korea Standard HRA method is 385. Among them, the number of individual post-accident human errors is 253. The number of dependent post-accident human errors is 135. The quantification results of the LPSD PSA model for the KSNP with new HEPs show that core damage frequency (CDF) is increased by 5.1% compared with the previous baseline CDF It is expected that this study results will be greatly helpful to improve the PSA quality for the domestic nuclear power plants because they have sufficient PSA quality to meet the Category II of Supporting Requirements for the post

  14. Studies on natural circulation in reactor loops under progressively depleted inventory conditions - post test analysis of ISP-33

    International Nuclear Information System (INIS)

    Nuclear reactors are designed for safety not only during normal operation, but also under abnormal/accident conditions. A number of accident scenarios are postulated and analysed using the computer codes developed for this purpose. The phenomena involved being complex, the codes need to be validated. The International Standard Problem exercise 33 (ISP-33) was conducted on the PACTEL test facility to validate and compare methodology for natural circulation phenomena under depleted inventory conditions. This paper presents the results of post-test calculation using ATHLET code in terms of flows, fluid temperatures, pressures in various nodes of the facility and describes the various modifications made for post-test analysis to improve the predictions. Pre-test/post-test predictions and experimental results are also compared. (author). 4 refs., 10 figs., 1 tab

  15. Chimeric External Control to Quantify Cell Free DNA in Plasma Samples by Real Time PCR

    Science.gov (United States)

    Eini, Maryam; Behzad-Behbahani, Abbas; Takhshid, Mohammad Ali; Ramezani, Amin; Rafiei Dehbidi, Gholam Reza; Okhovat, Mohammad Ali; Farhadi, Ali; Alavi, Parniyan

    2016-01-01

    Background: DNA isolation procedure can significantly influence the quantification of DNA by real time PCR specially when cell free DNA (cfDNA) is the subject. To assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfDNA, development of appropriate External DNA Control (EDC) is challenging. Using non-human chimeric nucleotide sequences, an EDC was developed for standardization of qPCR for monitoring stability of cfDNA concentration in blood samples over time. Methods: A0 DNA fragment of 167 bp chimeric sequence of parvovirus B19 and pBHA designated as EDC fragment was designed. To determine the impact of different factors during DNA extraction processing on quantification of cfDNA, blood samples were collected from normal subjects and divided into aliquots with and without specific treatment. In time intervals, the plasma samples were isolated. The amplicon of 167 bp EDC fragment in final concentration of 1.1 pg/500 μl was added to each plasma sample and total DNA was extracted by an in house method. Relative and absolute quantification real time PCR was performed to quantify both EDC fragment and cfDNA in extracted samples. Results: Comparison of real time PCR threshold cycle (Ct) for cfDNA fragment in tubes with and without specific treatment indicated a decrease in untreated tubes. In contrast, the threshold cycle was constant for EDC fragment in treated and untreated tubes, indicating the difference in Ct values of the cfDNA is because of specific treatments that were made on them. Conclusions: Spiking of DNA fragment size relevant to cfDNA into the plasma sample can be useful to minimize the bias due to sample preparation and extraction processing. Therefore, it is highly recommended that standard external DNA control be employed for the extraction and quantification of cfDNA for accurate data analysis.

  16. Efficient small sample analysis via laser post-ionized neutrals desorbed from surfaces: LPI-SNMS

    Science.gov (United States)

    Veryovkin, I.; Calaway, W.; Pellin, M.; Moore, J.; Burnett, D.

    2003-04-01

    A number of popular analytical techniques rely on ion sputtering or laser desorption to probe solid samples. The popularity of this class of techniques is derived from the fact that they produce information on elemental and molecular compositions at trace levels. These techniques are particularly amenable to small sample analysis, since both ion and photon beams can be focused to sub-micron dimensions. Because ion sputtering and laser desorption consume material, there exists a trade off between sample size and achievable detection limit. This trade off is quantified by an instruments useful yield, which is defined as the number of atoms detected per atoms consumed. Laser post-ionization secondary neutral mass spectrometry (LPI-SNMS) has useful yields significantly higher than competing techniques and is thus well suited for trace analysis of small samples. With LPI-SNMS, either a pulse of energetic ions or photons remove material from a solid surface into the gas phase. The desorbed material, predominantly ground state neutral atoms, is photo-ionized by one or more lasers and then extracted into a mass spectrometer for detection. At Argonne National Laboratory, we have developed a new reflectron time-of flight (TOF) mass spectrometer especially designed to optimize useful yield in LPI-SNMS measurements. Using ion optics simulations, an improved extraction design has been developed that allows photo ions from a large (4 × 4 × 3 mm^3) volume above a sample surface to be transmitted through a TOF mass spectrometer with > 98% efficiency. Efficient extraction from such a large ionization volume means that more than 40% of all desorbed species are available for detection, producing an overall useful yield of > 30%. Such a high sensitivity allows analysis of small samples at trace levels never before achievable, opening many new applications. For example, the new LPI-SNMS instrument will allow (1) part-per-trillion detections of solar wind elements implanted in the top

  17. Post-test analysis of OECD/NEA ROSA-2 test 4 using TRACE

    International Nuclear Information System (INIS)

    Experimental results obtained at integral test facilities are used in the validation process of thermal-hydraulic system codes for the steady-state and transient simulation of light water reactors. The expertise and guidelines derived from this work (notably the nodalization scheme and the specific model options) are later applied to safety analyses of nuclear power plants. This paper describes work carried out within the STARS project at the Paul Scherrer Institut (PSI) using the U.S. NRC-sponsored system code TRACE in the post-test analysis of the OECD/NEA ROSA-2 Test 4, a steam generator tube rupture (SGTR) accident test that was conducted at the Rig of Safety Assessment Large-Scale Testing Facility (ROSA/LSTF) of the Japan Atomic Energy Agency (JAEA). A custom in-house version of TRACE, based on version 5.0 release candidate 3, was used for the analysis. The ROSA/LSTF model is based on an existing model developed at PSI, which was previously validated over a range of seven ROSA/LSTF loss-of-coolant and steam line rupture tests conducted as part of the ROSA and ROSA-2 OECD programs. In general there is a reasonable agreement between the simulation results and experimental values. The TRACE model tends to overpredict the steam-generator liquid levels. This has affected the timing of several events during the transient. The mass flow rate through the ruptured U-tube is slightly underpredicted in the choked flow regime but is captured accurately at lower pressures. Mass flow rates and temperatures in the hot and cold legs are accurately reproduced. The TRACE model was unable to capture the temperature stratification caused by the injection of cold water into the cold leg connected to the damaged steam generator. This phenomenon, common to thermal-hydraulic system codes, was previously identified by the experts from the Operating Agent (JAEA). This work provides a valuable addition to the STARS validation database for the analysis of the safety of light water

  18. Finite element stress analysis of short-post core and over restorations prepared with different restorative materials.

    Science.gov (United States)

    Gurbuz, Taskin; Sengul, Fatih; Altun, Ceyhan

    2008-07-01

    The present study was conducted to determine the effect on the distribution of stress with the use of short-post cores and over restorations composed of different materials. The restorative materials used were namely two different composite resin materials (Valux Plus and Tetric Flow), a polyacid-modified resin material (Dyract AP), and a woven polyethylene fiber combination (Ribbond Fiber + Bonding agent + Tetric Flow). Finite element analysis (FEA) was used to develop a model for the maxillary primary anterior teeth. A masticatory force of 100 N was applied at 148 degrees to the incisal edge of the palatal surface of the crown model. Stress distributions and stress values were compared using von Mises criteria. The tooth model was assumed to be isotropic, homogeneous, elastic, and asymmetrical. It was observed that the highest stress usually occurred in the cervical area of the tooth when Tetric Flow was used as the short-post core and over restoration material. The same maximum stress value was also obtained when Ribbond fiber + Tetric Flow material was used for the short-post core. The results of FEA showed that the mechanical properties and elastic modulus of the restorative material influenced the stresses generated in enamel, dentin, and restoration when short-post core restorations were loaded incisally. Resin-based restorative materials with higher elastic moduli were found to be unsuitable as short-post core materials in endodontically treated maxillary primary anterior teeth. PMID:18833762

  19. Evaluation of Temperature and Stress Distribution on 2 Different Post Systems Using 3-Dimensional Finite Element Analysis

    Science.gov (United States)

    Değer, Yalçın; Adigüzel, Özkan; Özer, Senem Yiğit; Kaya, Sadullah; Polat, Zelal Seyfioğlu; Bozyel, Bejna

    2015-01-01

    Background The mouth is exposed to thermal irritation from hot and cold food and drinks. Thermal changes in the oral cavity produce expansions and contractions in tooth structures and restorative materials. The aim of this study was to investigate the effect of temperature and stress distribution on 2 different post systems using the 3-dimensional (3D) finite element method. Material/Methods The 3D finite element model shows a labio-lingual cross-sectional view of the endodontically treated upper right central incisor and supporting periodontal ligament with bone structures. Stainless steel and glass fiber post systems with different physical and thermal properties were modelled in the tooth restored with composite core and ceramic crown. We placed 100 N static vertical occlusal loading onto the center of the incisal surface of the tooth. Thermal loads of 0°C and 65°C were applied on the model for 5 s. Temperature and thermal stresses were determined on the labio-lingual section of the model at 6 different points. Results The distribution of stress, including thermal stress values, was calculated using 3D finite element analysis. The stainless steel post system produced more temperature and thermal stresses on the restorative materials, tooth structures, and posts than did the glass fiber reinforced composite posts. Conclusions Thermal changes generated stresses in the restorative materials, tooth, and supporting structures. PMID:26615495

  20. Site-specific climate analysis elucidates revegetation challenges for post-mining landscapes in eastern Australia

    OpenAIRE

    Audet, P.; Arnold, S.; A. M. Lechner; T. Baumgartl

    2013-01-01

    In eastern Australia, the availability of water is critical for the successful rehabilitation of post-mining landscapes and climatic characteristics of this diverse geographical region are closely defined by factors such as erratic rainfall and periods of drought and flooding. Despite this, specific metrics of climate patterning are seldom incorporated into the initial design of current post-mining land rehabilitation strategies. Our study proposes that a few common rainfall...

  1. Stress Analysis of a Maxillary Central Incisor Restored with Different Posts

    OpenAIRE

    Adanir, Necdet; Belli, Sema

    2007-01-01

    Objectives To evaluate the effects of different post materials on the stress distribution in an endodontically treated maxillary incisor. Materials and Methods A pseudo 3-dimensional finite element model was created in a labiolingual cross-sectional view of a maxillary central incisor and modified according to five posts with different physical properties consisting stainless steel, titanium, gold alloy, glass fiber (Snowpost), and carbon fiber (Composipost). A 200 N force was then applied fr...

  2. Economic Analysis of Post-harvest Losses in Marketing of Vegetables in Uttarakhand

    OpenAIRE

    Sharma, Gaurav; Singh, S.P.

    2011-01-01

    The study has examined the nature and extent of post-harvest losses in vegetable supply chain in the Kumaon division of Uttarakhand. Multistage cluster sampling has been used for selection of 80 vegetable growers, 40 farmers from the hilly region and 40 farmers from the bhabhar region. The sample has also included 25 market functionaries. Twelve major vegetables have been selected for the study. The maximum aggregate post-harvest losses have been found in tomato, followed by potato, brinjal, ...

  3. An adaptive model reduction strategy for post-buckling analysis of stiffened structures

    OpenAIRE

    Barrière, Ludovic; Marguet, Steven; Castanié, Bruno; Cresta, Philippe; Passieux, Jean-Charles

    2013-01-01

    The finite element simulation of structures subjected to post-buckling still faces computational limits, especially for large stiffened structures. Several solving strategies have already been proposed in response to this issue. Among them are the adaptive model reduction solving techniques which demonstrated their ability to drastically reduce the number of unknowns as well as to control the approximation error of solving non-linear problems like post-buckling. The challenges regarding these...

  4. Multi-level post-processing for Korean character recognition using morphological analysis and linguistic evaluation

    OpenAIRE

    Lee, Geunbae; Lee, Jong-Hyeok; Yoo, Jinhee

    1996-01-01

    Most of the post-processing methods for character recognition rely on contextual information of character and word-fragment levels. However, due to linguistic characteristics of Korean, such low-level information alone is not sufficient for high-quality character-recognition applications, and we need much higher-level contextual information to improve the recognition results. This paper presents a domain independent post-processing technique that utilizes multi-level morphological, syntactic,...

  5. Business Startups: An analysis of selection and post-startup performance (Japanese)

    OpenAIRE

    Yasuda, Takehiko

    2010-01-01

    This paper investigates how the pre-startup circumstances surrounding an entrepreneur, during the time when he or she decides to start a business, affects the post-startup performance. Based on the original dataset, sample selection models are estimated connecting nascent entrepreneurs' selection of a startup with actual entrepreneurs' performance post-startup. The results of this paper confirm that the individual attributes of nascent entrepreneurs, which make it easier (difficult) to decide...

  6. Analysis of the of customer service provided at the Mafikeng Post Office / Maria Matshidiso Mosala

    OpenAIRE

    Mosala, Maria Matshidiso

    2006-01-01

    The study set to investigate customer care in the Mafikeng Post Office. This was achieved through the following. Highlighting any inadequacy of customer care process at Mafikeng Post Office, determining if the current customer care tools utilised are optimal or not, determining the extent at which such tools are utilised and determining where accountability lies for customer care within the organisation. The measuring instruments utilised was a questionnaire because of the d...

  7. The developmental fate of green fluorescent mouse embryonic germ cells in chimeric embryos

    Institute of Scientific and Technical Information of China (English)

    XUXIN; SUMIOSUGANO; 等

    1999-01-01

    Primordial germ cells (PGCs),as precursors of mammalian germ lineage,have been gaining more attention as a new resource of pluripotent stem cells,which bring a great possibility to study developmental events of germ cell in vitro and at animal level.EG4 cells derived from 10.5 days post coitum (dpc) PGCs of 129/svJ strain mouse were established and maintained in an undifferentiated state.With an attempt to study the differentiation capability of EG4 cells with a reporter protein:green fluorescence protein,and the possible application of EG4 cells in the research of germ cell development,we have generated several EG4-GFP cell lines expressing enhanced green fluorescence protein (EGFP) and still maintaining typical characteristics of pluripotent stem cells.Then,the differentiation of EG4-GFP cells in vitro as well as their developmental fate in chimeric embryos which were produced by aggregating EG4-GFP cells to 8-cell stage embryos were studied.The results showed that EG4 cells carrying green fluorescence have a potential use in the research of germ cell development and other related studies.

  8. GPR signal analysis of post-tensioned prestressed concrete girder defects

    International Nuclear Information System (INIS)

    The accurate inspection of the duct condition in post-tensioned prestressed concrete (PPC) is an essential part of GPR concrete inspection. The purpose is to inspect the grouting condition of the ducts where the strands are located, to find out if there is a void in the ducts, and if any water exists. In order to investigate the radar image characteristics of different PPC duct defects, a number of model girders were manufactured. Three major ducts are included in our study: (1) well grouted and no void (normal condition); (2) the duct is half filled, and the void is filled by water or air; and (3) the duct is not filled at all, and the duct is water or air filled. The data corresponding to seven different situations are acquired and processed. It is found that the radar can detect the first interface in the duct, and the detailed structure inside the duct cannot be ‘seen’ from the images directly. Characteristic curves greatly help the interpretation. A completely void duct is the easiest to differentiate from the others. The signature for this situation is characterized by a strong and clear reflection interface which becomes weaker as the void is water filled. The normal condition shows the weakest reflection interface. As for the half void situation, the front scan shows a similar result to the normal condition whether it is water or air filled, and the back scan shows similar features to the completely void situation. The experiment and analysis is helpful and instructive for practical engineering inspection. (paper)

  9. Post-radiation changes in oral tissues - An analysis of cancer irradiation cases

    Directory of Open Access Journals (Sweden)

    Jay Ashokkumar Pandya

    2014-01-01

    Full Text Available Introduction: Radiation, commonly employed as neoadjuvant, primary, and adjuvant therapy for head and neck cancer causes numerous epithelial and stromal changes, prominent among which is fibrosis with its early and late consequences. Very little is known about the true nature of the fibrosed tissue and the type of fibers accumulated. Radiotherapy affects the supporting tumor stroma often resulting in a worsening grade of tumor post-radiation. Aim: To study epithelial, neoplastic, stromal, and glandular changes in oral cavity induced by radiation therapy for oral squamous cell carcinoma (OSCC using special stains. Materials and Methods: The study included 27 samples of recurrent OSCC following completion of radiotherapy (recurrence within an average span of 11 months, and 26 non-irradiated cases of OSCC. Patients with a history of combined radiotherapy and chemotherapy were not included in the study. The epithelial changes assessed included epithelial atrophy, apoptosis, necrosis, dysplasia, and neoplasia. The connective tissue was evaluated for amount of fibrosis, quality of fibers (using picrosirius red staining, fibrinous exudate, necrosis, pattern of invasion, vessel wall thickening, and salivary gland changes. The aforementioned changes were assessed using light and polarizing microscopy and tabulated. Statistical Analysis: Epithelial and connective tissue parameters were compared between the irradiated and non-irradiated cases using chi square and t-tests. Results: Epithelial and connective tissue parameters were found to be increased in irradiated patients. Pattern of invasion by tumor cells varied from strands and  cords between the two groups studied. The effect of radiation was seen to reflect on the maturity of fibers and the regularity of their distribution.

  10. Analysis of changes in post-seismic landslide distribution and its effect on building reconstruction

    Science.gov (United States)

    Yang, W. T.; Wang, M.; Kerle, N.; Van Westen, C. J.; Liu, L. Y.; Shi, P. J.

    2015-04-01

    Six years after the devastating Ms 8.0 Wenchuan earthquake, new landslides, debris flows, and flash floods still occur frequently in the earthquake-stricken regions. This shows that the geological hazards that occur after a major earthquake in a mountainous environment can be a long-term threat. However, post-earthquake reconstruction and relocation of local residents often neglect this evolving threat, and its interaction with existing and rebuilt houses has not been well studied. Here we show that the evolving mountain environment, including the changed geographic distribution of new landslides and the continuously uplifting riverbed, creates emerging risks for existing and rebuilt houses. We use spatial analysis of landslide debris and the location of houses from high-resolution images and field survey in the study area and find that new landslides and the houses rebuilt after the Wenchuan earthquake have a similar trend of moving to lower elevations, gentler slopes, and closer to rivers. This study confirms that the persistent downward movement of landslide debris has rapidly filled up riverbeds over the past 6 years. The elevated riverbeds make the study area extremely susceptible to flash floods, creating further risks to newly rebuilt houses that are closer to the river. We highlight the often neglected dynamic process that involves changes in the natural environment and man-made constructions and their interaction. This dynamic process requires long-term monitoring and adaptive management of mountainous regions after major earthquakes that can fully consider the sophisticated evolving risks caused by the changing environment, exposure, and vulnerability in the region.

  11. Analysis of changes in post-seismic landslide distribution and its effect on building reconstruction

    Directory of Open Access Journals (Sweden)

    W. T. Yang

    2014-08-01

    Full Text Available Six years after the devastating Ms 8.0 Wenchuan Earthquake, new landslides, debris flows, and flash floods still occur frequently in the earthquake-stricken regions. This shows the geological hazards that occur after a major earthquake in a mountainous environment can be a long-term threat. However, post-earthquake reconstruction and relocation of local residents often neglect this evolving threat, and its interaction with existing and rebuilt houses has not been well studied. Here we show that the evolving mountain environment, including the changed geographic distribution of new landslides and the continuously uplifting riverbed, creates emerging risks for existing and rebuilt houses. We use spatial analysis of landslide debris and the location of houses from high-resolution images and field survey in the study area, and find that new landslides and the houses rebuilt after the Wenchuan Earthquake have a similar trend of moving to lower elevations, gentler slopes, and closer to rivers. This study confirms that the persistent downward movement of landslide debris has rapidly filled up riverbeds over the past 6 yr. The elevated riverbeds make the study area extremely susceptible to flash floods, creating further risks to newly rebuilt houses that are closer to the river. We highlight the often neglected dynamic process that involves changes in the natural environment and man-made constructions, and their interaction. This dynamic process requires long-term monitoring and adaptive management of mountainous regions after major earthquakes that can fully consider the sophisticated evolving risks caused by the changing environment, exposure and vulnerability in the region.

  12. RICE IN COLONIAL AND POST COLONIAL SOUTHEAST ASIA: A FOOD REGIME ANALYSIS

    Directory of Open Access Journals (Sweden)

    Dias Pradadimara

    2015-01-01

    Full Text Available This paper traces the ways in which rice, as a global commodity, has been produced and sold in various regions in Southeast Asia from the colonial era to the present days. This paper employs a food regime analysis first introduced by Harriet Friedmann (1982 and later developed together with Philip McMichael (1989 to look at the global political economy of rice. In this paper, it will be shown how various colonial and post colonial states in Southeast Asia (including Thailand who was never formally colonized through their policies have practically divided the region where Burma (now Myanmar, Thailand and Vietnam in the mainland have become major rice producer and exporter, while Indonesia, Malaya (now Malaysia, and the Philippines in the archipelagic Southeast Asia have become major rice importers although at the same time producers and exporters of other agro-commodities (coffee, sugar, rubber.Keywords: rice history, food regime, Southeast Asia Artikel ini menelusuri cara di mana padi sebagai komoditas dunia diproduksi dan dijual di beberapa daerah di Asia Tenggara mulai zaman kolonial sampai sekarang. Artikel ini menggunakan analisis “food regime” yang pertama kali diperkenalkan oleh Harrier Friedman (1982 dan kemudian dikembangkan bersama oleh Philip (1989 untuk mengetahui politik ekonomi global dari padi. Dalam artikel ini, akan dilihat mengenai bagaimana negara kolonial dan pasca-kolonial yanb berbeda di Asia Tenggara (termasuk Thailand yang tidak pernah dijajah sebelumnya melalui kebiakannya, yang hampir membagi wilayahnya, di mana Burma (Myanmar, Thailand, dan Vietnam telah menjadi produsen dan eksportir utama terbesar, sedangkan Indonesia dan Malaya (Malaysia dan Filipina di Asia Tenggara telah menjadi produsen dan eksportir komoditas pertanian lain (kopi, gula, karet dalam waktu yang bersamaan.Kata kunci: sejarah beras, food regime, Asia Tenggara. 

  13. Insidious Colonialism in Post-Apartheid Education: Interplay of Black Teacher Narratives, Educational Policy and Textbook Analysis

    Directory of Open Access Journals (Sweden)

    Sharon Subreemduth

    2013-10-01

    Full Text Available This article focuses on the larger project of identifying oppressive structures (during apartheid more specifically in this instance and how educational policy/textbooks (post-apartheid produce transformative knowledge for decolonization. It presents Black South African teacher perceptions and desires of what/how educational policy, history textbooks can intervene in apartheid indoctrination and what role these have in addressing the nation’s meta-narrative of equity and social justice. I take up these teacher narratives as a way to further critique textbooks currently used and examine the written and visual content against post-apartheid decolonizing intentions. Teacher narratives and textbook analysis indicate that even prescriptive post-apartheid textbooks struggle to reimagine history wrought through with colonialism. Decolonizing analysis of visual images in the textbooks show how curriculum policy/practice in South Africa is a collision of anti-apartheid desires and post-apartheid reality. By examining grassroots linkages I attempt to expand the current dialogue on educational policy and textbook studies and to contextualize the field, both historically and contemporarily.

  14. Post-treatment of molasses wastewater by electrocoagulation and process optimization through response surface analysis.

    Science.gov (United States)

    Tsioptsias, C; Petridis, D; Athanasakis, N; Lemonidis, I; Deligiannis, A; Samaras, P

    2015-12-01

    Molasses wastewater is a high strength effluent of food industry such as distilleries, sugar and yeast production plants etc. It is characterized by a dark brown color and exhibits a high content in substances of recalcitrant nature such as melanoidins. In this study, electrocoagulation (EC) was studied as a post treatment step for biologically treated molasses wastewater with high nitrogen content obtained from a baker's yeast industry. Iron and copper electrodes were used in various forms; the influence and interaction of current density, molasses wastewater dilution, and reaction time, on COD, color, ammonium and nitrate removal rates and operating cost were studied and optimized through Box Behnken's response surface analysis. Reaction time varied from 0.5 to 4 h, current density varied from 5 to 40 mA/cm(2) and dilution from 0 to 90% (v/v expressed as water concentration). pH, conductivity and temperature measurements were also carried out during each experiment. From preliminary experiments, it was concluded that the application of aeration and sample dilution, considerably influenced the kinetics of the process. The obtained results showed that COD removal varied between 10 and 54%, corresponding to an operation cost ranging from 0.2 to 33 euro/kg COD removed. Significant removal rates were obtained for nitrogen as nitrate and ammonium (i.e. 70% ammonium removal). A linear relation of COD and ammonium to the design parameters was observed, while operation cost and nitrate removal responded in a curvilinear function. A low ratio of electrode surface to treated volume was used, associated to a low investment cost; in addition, iron wastes could be utilized as low cost electrodes i.e. iron fillings from lathes, aiming to a low operation cost due to electrodes replacement. In general, electrocoagulation proved to be an effective and low cost process for biologically treated molasses-wastewater treatment for additional removal of COD and nitrogen content and

  15. Ex-post evaluation of safety research by technology interaction analysis

    International Nuclear Information System (INIS)

    By using the database INSPEC (Information Services for Physics, Electronics and Computing), an ex-post evaluation of the safety research was carried out by using a technology interaction analysis. Selected prestigious nuclear institutions were JAERI (Japan Atomic Energy Research Institute, Japan), ORNL (Oak Ridge National Laboratory, The U.S.) and FZK (Forschungszentrum Karlsruhe, Germany). As the control term of INSPEC the water-reactor-safety was used to study the established key technologies and the interacted technological elements. (1) For a nuclear safety research in Japan, 30 out of 274 key technologies were examined and revealed that JAERI held priority to 22 key technologies, consisted of the nuclear fuels, the nuclear materials, the thermal hydraulics, the accidents and the research reactors. Hence, JAERI contributed much to Japan. As to ORNL, they held priority to 19 key technologies, represented by the nuclear power plant (NPP) operation and maintenance/inspection. They were however inferior to the key technology such as thermal hydraulics. As to FZK, they held priority to 20 key technologies, represented by a radiation protection. (2) For selected 20 key technologies, a half of them were held in common by the three institutions. They were the NPP (including engineering calculation etc.), the nuclear fuels, the nuclear materials, and the accidents (including a reactor core cooling and heat removal). Those technologies were unavoidable for the stable and the sustainable usage of NPP. (3) Among the three nations, 13 technological elements consisted of the NPP (including reactor theory and design etc.), the nuclear fuels, the nuclear materials, and the accidents (including a reactor core cooling and heat removal) were held in common Regarding the items of key technologies there is no difference between the level of nation and that of institution. (4) Key technologies held in common at domestic and institutional level were, 11 for JAERI, 5 for ORNL and 7

  16. Construction and characterization of chimeric BHIV (BIV/HIV-1) viruses carrying the bovine immunodeficiency virus gag gene

    Institute of Scientific and Technical Information of China (English)

    Yi-Xin Zhu; Chang Liu; Xin-Lei Liu; Wen-Tao Qiao; Qi-Min Chen; Yi Zeng; Yun-Qi Geng

    2005-01-01

    AIM: To explore the possibility of the replacement of the gag gene between human immunodeficiency virus and bovine immunodeficiency virus, to achieve chimeric virions,and thereby gain a new kind of AIDS vaccine based on BHIV chimeric viruses.METHODS: A series of chimeric BHIV proviral DNAs differing in the replacement regions in gag gene were constructed, and then were transfected into 293T cells. The expression of chimeric viral genes was detected at the RNA and protein level. The supematant of 293T cell was ultra centrifuged to detect the probable chimeric virion. Once the chimeric virion was detected, its biological activities were also assayed by infecting HIV-sensitive MT4 cells.RESULTS: Four chimeric BHIV proviral DNAs were constructed. Genes in chimeric viruses expressed correctly in transfected 293T cells. All four constructs assembled chimeric virions with different degrees of efficiency. These virions had complete structures common to retroviruses and packaged genomic RNAs, but the cleavages of the precursor Gag proteins were abnormal to some extent. Three of these virions tested could attach and enter into MT4 cells, and one of them could complete the course of reverse transcription. Yet none of them could replicate in MT4 cells.CONCLUSION: The replacement of partial gag gene of HIV with BIV gaggene is feasible. Genes in chimeric BHIVs are accurately expressed, and virions are assembled. These chimeric BHIVs (proviral DNA together with virus particles) have the potential to become a new kind of HIV/AIDS vaccine.

  17. Modeling cognition and disease using human glial chimeric mice

    DEFF Research Database (Denmark)

    Goldman, Steven A.; Nedergaard, Maiken; Windrem, Martha S.

    2015-01-01

    cognition and information processing. In addition, the cellular humanization of these brains permits their use in studying glial infectious and inflammatory disorders unique to humans, and the effects of those disorders on the glial contributions to cognition. Perhaps most intriguingly, by pairing our...... ability to construct human glial chimeras with the production of patient-specific hGPCs derived from pluripotential stem cells, we may now establish mice in which a substantial proportion of resident glia are both human and disease-derived. These mice in particular may provide us new opportunities for...... studying the human-specific contributions of glia to psychopathology, as well as to higher cognition. As such, the assessment of human glial chimeric mice may provide us new insight into the species-specific contributions of glia to human cognitive evolution, as well as to the pathogenesis of human...

  18. Chimeric Antigen Receptors Modified T-Cells for Cancer Therapy.

    Science.gov (United States)

    Dai, Hanren; Wang, Yao; Lu, Xuechun; Han, Weidong

    2016-07-01

    The genetic modification and characterization of T-cells with chimeric antigen receptors (CARs) allow functionally distinct T-cell subsets to recognize specific tumor cells. The incorporation of costimulatory molecules or cytokines can enable engineered T-cells to eliminate tumor cells. CARs are generated by fusing the antigen-binding region of a monoclonal antibody (mAb) or other ligand to membrane-spanning and intracellular-signaling domains. They have recently shown clinical benefit in patients treated with CD19-directed autologous T-cells. Recent successes suggest that the modification of T-cells with CARs could be a powerful approach for developing safe and effective cancer therapeutics. Here, we briefly review early studies, consider strategies to improve the therapeutic potential and safety, and discuss the challenges and future prospects for CAR T-cells in cancer therapy. PMID:26819347

  19. Confined Blood Chimerism in Monochorionic Dizygotic Twins Conceived Spontaneously

    Directory of Open Access Journals (Sweden)

    Takashi Kanda

    2013-05-01

    Full Text Available Traditionally, monochorionicity has been regarded as synonymous with monozygosity. However, several recent cases of monochorionic dizygotic twins have shown that monochorionic twins can be dizygous. We report a rare case of monochorionic diamnionic, gender-discordant twins who were conceived spontaneously. Initially, a monochorionic placenta was diagnosed by ultrasonography at 8 weeks of gestation and then confirmed by pathology after delivery. The twins had different genders. A comparison of cytogenetic analyses using peripheral blood lymphocytes and skin fibroblasts revealed that chimerism was confined to blood cells. We have experienced two cases of monochorionic dizygotic twins since 2003. These cases suggest that monochorionic dizygotic twins are not as rare as previously thought.

  20. Persistence of chicken herpesvirus and retroviral chimeric molecules upon in vivo passage.

    Science.gov (United States)

    Borenshtain, R; Witter, R L; Davidson, I

    2003-01-01

    Mareks disease virus (MDV), a herpesvirus, and avian leucosis virus subgroup J (ALV-J), a retrovirus, were used for experimental coinfection of chickens. Chimeric molecules having sequences of both viruses were detected by the hotspot-combined polymerase chain reaction (HS-cPCR) system. The detection of chimeric molecules provided evidence for avian retroviral inserts in the herpesvirus genome. The persistence of chimeric molecules on in vivo passage served to indicate the infectivity of the recombinant virus. The evaluation of formation and persistence of the chimeric molecules was performed in two trials involving three in vivo passages. The chimeric molecules were identified according to the primer sets, their product length, and pattern. The persistence of chimeric molecules on in vivo passages served as an indication of their ability to replicate in and infect chickens. In the first experimental passage, MDV and ALV-J prototype strains, MD11 and HC-1, were intraperitoneally (i.p.) injected into 1-day-old chicks. The second trial included two passages. Passage II chicks were injected i.p. and passage III chickens were in contact with the chickens of passage II. For passage II, enriched white blood cells from blood samples of chickens from the first trial that had chimeric molecules were injected i.p. into 1-day-old chicks. For passage III, uninfected chicks were included together with the infected chicks. Synthesis evidence for the various species of chimeric molecules was assessed in the tissues of birds of the second trial. DNA was extracted from blood and feathers and analyzed by the hotspot-combined PCR and by pulsed field gel electrophoresis. To overcome the limits of detection, three amplification assays followed by hybridization of the products to specific viral probes were conducted. A variety of chimeric molecules were detected in low concentrations. Five species of chimeric molecules were characterized in blood, tumors, and feathers. Chimeric

  1. The contribution of turmeric research and development In the economy of Bangladesh: an ex-post analysis

    OpenAIRE

    MK Hasan

    2014-01-01

    The study estimated the benefit and rates of returns to investment on turmeric research and development in Bangladesh. The Economic Surplus Model with ex-post analysis was used to determine the returns to investment and their distribution between the production and consumption. Several discounting techniques were also used to assess the efficiency of turmeric research. The adoption rate was found increasing trend over the period. The yield of BARI developed modern varieties of turmeric was 41...

  2. MS1 Peptide Ion Intensity Chromatograms in MS2 (SWATH) Data Independent Acquisitions. Improving Post Acquisition Analysis of Proteomic Experiments*

    OpenAIRE

    Rardin, Matthew J.; Schilling, Birgit; Cheng, Lin-Yang; MacLean, Brendan X.; Sorensen, Dylan J.; Sahu, Alexandria K.; MacCoss, Michael J; Vitek, Olga; Gibson, Bradford W.

    2015-01-01

    Quantitative analysis of discovery-based proteomic workflows now relies on high-throughput large-scale methods for identification and quantitation of proteins and post-translational modifications. Advancements in label-free quantitative techniques, using either data-dependent or data-independent mass spectrometric acquisitions, have coincided with improved instrumentation featuring greater precision, increased mass accuracy, and faster scan speeds. We recently reported on a new quantitative m...

  3. A Post Hoc Analysis of D-Threo-Methylphenidate Hydrochloride (Focalin) Versus D,l-Threo-Methylphenidate Hydrochloride (Ritalin)

    Science.gov (United States)

    Weiss, Margaret; Wasdell, Michael; Patin, John

    2004-01-01

    Objective: To evaluate clinical measures of the benefit/risk ratio in a post hoc analysis of a clinical trial of d-threo-methylphenidate hydrochloride (d-MPH) and d,l-threo-methylphenidate hydrochloride (d,l-MPH). Method: Data from a phase III clinical trial was used to compare equimolar doses of d-MPH and d,l-MPH treatment for…

  4. A meta-analysis of prevalence rates and moderating factors for cancer-related post-traumatic stress disorder

    OpenAIRE

    Abbey, Gareth; Thompson, Simon B N; Hickish, Tamas; Heathcote, David

    2014-01-01

    Objective Systematic reviews highlight a broad range of cancer-related post-traumatic stress disorder (CR-PTSD) prevalence estimates in cancer survivors. This meta-analysis was conducted to provide a prevalence estimate of significant CR-PTSD symptoms and full diagnoses to facilitate the psychological aftercare of cancer survivors. Methods A systematic literature search was conducted for studies using samples of cancer survivors by using validated clinical interviews and questionnaires to ass...

  5. Analysis of post-blasting source mechanisms of mining-induced seismic events in Rudna copper mine, Poland

    OpenAIRE

    Caputa Alicja; Talaga Adam; Rudziński Łukasz

    2015-01-01

    The exploitation of georesources by underground mining can be responsible for seismic activity in areas considered aseismic. Since strong seismic events are connected with rockburst hazard, it is a continuous requirement to reduce seismic risk. One of the most effective methods to do so is blasting in potentially hazardous mining panels. In this way, small to moderate tremors are provoked and stress accumulation is substantially reduced. In this paper we present an analysis of post-blasting e...

  6. Polypharmacy and effects of apixaban versus warfarin in patients with atrial fibrillation: post hoc analysis of the ARISTOTLE trial

    OpenAIRE

    Jaspers Focks, Jeroen; Brouwer, Marc A; Wojdyla, Daniel M; Thomas, Laine; Lopes, Renato D.; Washam, Jeffrey B.; Lanas, Fernando; Xavier, Denis; Husted, Steen; Wallentin, Lars; Alexander, John H; Granger, Christopher B; Verheugt, Freek W A

    2016-01-01

    Objective To determine whether the treatment effect of apixaban versus warfarin differs with increasing numbers of concomitant drugs used by patients with atrial fibrillation. Design Post hoc analysis performed in 2015 of results from ARISTOTLE (apixaban for reduction in stroke and other thromboembolic events in atrial fibrillation)—a multicentre, double blind, double dummy trial that started in 2006 and ended in 2011. Participants 18 201 ARISTOTLE trial participants. Interventions In the ARI...

  7. Hodgkin lymphoma post-transplant lymphoproliferative disorder: A comparative analysis of clinical characteristics, prognosis, and survival.

    Science.gov (United States)

    Rosenberg, Aaron S; Klein, Andreas K; Ruthazer, Robin; Evens, Andrew M

    2016-06-01

    Hodgkin lymphoma post-transplant lymphoproliferative disorder (HL-PTLD) is an uncommon PTLD with unclear prognosis and differences between HL-PTLD and immunocompetent HL are not well defined. Patient characteristics were compared among 192 patients with HL-PTLD from the Scientific Registry of Transplant Recipients and 13,847 HL patients in SEER (HL-SEER). Overall survival (OS) and disease-specific survival (DSS) were compared after exact matching. Additionally, multivariable analyses were used to identify prognostic markers of survival and associations between treatment and survival. Median time from transplant to HL-PTLD diagnosis was 88 months. When compared with HL-SEER, patients with HL-PTLD were older (median age, 52 vs. 36 years, P = 0.001), more likely male (73% vs. 54%, P < 0.001), Caucasian (81% vs. 70%, P = 0.02), and had extranodal disease (42% vs. 3%, P < 0.001). Five-year OS for patients with HL-PTLD was 57% versus 80% for HL-SEER (P < 0.001); DSS was also inferior (P < 0.001). For patients with HL-PTLD, the use of any chemotherapy was associated with decreased hazard of death (HR = 0.36, P < 0.001). Furthermore, patients who received no chemotherapy or nontraditional HL regimens had increased hazard of death (aHR = 2.94, P = 0.001 and 2.01, P = 0.04) versus HL-specific chemotherapy regimens. In multivariable analysis, advanced age and elevated creatinine were associated with inferior OS (aHR = 1.26/decade P < 0.001 and 1.64/0.1 mg/dL increase P = 0.02). A prognostic score based on the number of these adverse factors (0, 1, 2) was associated with 10-year OS rates of 79%, 53%, and 11%, respectively (P < 0.001). Altogether, HL-PTLD patients have inferior survival when compared with HL-SEER. Furthermore, treatment with HL-specific chemotherapy was associated with improved OS, whereas age and creatinine identified patients with markedly divergent survival. Am. J. Hematol. 91:560-565, 2016. © 2016

  8. Prediction and Analysis of Post-Translational Pyruvoyl Residue Modification Sites from Internal Serines in Proteins.

    Directory of Open Access Journals (Sweden)

    Yang Jiang

    Full Text Available Most of pyruvoyl-dependent proteins observed in prokaryotes and eukaryotes are critical regulatory enzymes, which are primary targets of inhibitors for anti-cancer and anti-parasitic therapy. These proteins undergo an autocatalytic, intramolecular self-cleavage reaction in which a covalently bound pyruvoyl group is generated on a conserved serine residue. Traditional detections of the modified serine sites are performed by experimental approaches, which are often labor-intensive and time-consuming. In this study, we initiated in an attempt for the computational predictions of such serine sites with Feature Selection based on a Random Forest. Since only a small number of experimentally verified pyruvoyl-modified proteins are collected in the protein database at its current version, we only used a small dataset in this study. After removing proteins with sequence identities >60%, a non-redundant dataset was generated and was used, which contained only 46 proteins, with one pyruvoyl serine site for each protein. Several types of features were considered in our method including PSSM conservation scores, disorders, secondary structures, solvent accessibilities, amino acid factors and amino acid occurrence frequencies. As a result, a pretty good performance was achieved in our dataset. The best 100.00% accuracy and 1.0000 MCC value were obtained from the training dataset, and 93.75% accuracy and 0.8441 MCC value from the testing dataset. The optimal feature set contained 9 features. Analysis of the optimal feature set indicated the important roles of some specific features in determining the pyruvoyl-group-serine sites, which were consistent with several results of earlier experimental studies. These selected features may shed some light on the in-depth understanding of the mechanism of the post-translational self-maturation process, providing guidelines for experimental validation. Future work should be made as more pyruvoyl-modified proteins are

  9. Analysis of Pre- and Post-Monsoon Suspended Sediments in the Gulf of Kachchh, India Using Remote Sensing

    CERN Document Server

    Gupta, Mukesh

    2015-01-01

    A comprehensive study of satellite-derived suspended sediment concentration (SSC) during pre- and post-monsoon has been conducted with full-month cycles of tidal responses to study the suspended sediment dynamics in the Gulf of Kachchh. Tidal data were interpreted in conjunction with the OCEANSAT-1 ocean color monitor (OCM)-derived SSC for pre- and post-monsoon. The analysis of the data shows that the Gulf is predominantly affected by the tidal changes. The average SSC during pre-monsoon were 30.8 mg/l (high tide) and 24.1 mg/l (low tide); and during post-monsoon 19.7 mg/l (high tide) and 21.8 mg/l (low tide). The only little monsoonal influence is seen when Indus River discharges sediments during pre-monsoon due to increased sediment flux from its origin, Himalayas in spring (February-April) as compared to less sediment discharge observed during winter (November-December). The pre-monsoon SSC images show overall high suspended sediments whereas post-monsoon SSC images show comparatively low SSC. The use of e...

  10. A Meta-analysis of Imagery Rehearsal for Post-trauma Nightmares: Effects on Nightmare Frequency, Sleep Quality, and Posttraumatic Stress

    OpenAIRE

    Casement, Melynda D.; Swanson, Leslie M.

    2012-01-01

    This meta-analysis evaluates the efficacy of imagery rehearsal as a treatment for nightmares, general sleep disturbance, and symptoms of post-traumatic stress. Bibliographic databases and cited references were searched to identify clinical trials of imagery rehearsal in individuals with post-trauma nightmares. Thirteen studies met inclusion criteria and reported sleep and post-traumatic stress outcomes in sufficient detail to calculate effect sizes. Results indicate that imagery rehearsal had...

  11. Adjusted prognostic association of post-myocardial infarction depression with mortality and cardiovascular events: an individual patient data meta-analysis.

    OpenAIRE

    Meijer, Anna; Conradi, Henk Jan; Bos, Elske H; Anselmino, Matteo; Carney, Robert M.; Denollet, Johan; Doyle, Frank; Freedland, Kenneth E.; Grace, Sherry L; Hosseini, Seyed Hamzeh; Lane, Deirdre A.; Pilote, Louise; Parakh, Kapil; Rafanelli, Chiara; Sato, Hiroshi

    2013-01-01

    Background: The association between depression after myocardial infarction (post-MI) and increased risk of mortality and cardiac morbidity may be due to cardiac disease severity. Aims: To combine original data from studies on the association between post-MI depression and prognosis into one database. To investigate to what extent post-MI depression predicts prognosis independently of disease severity. Method: Individual patient data meta-analysis of studies, using multilevel, multivaria...

  12. Supportive Distortions: An Analysis of Posts on a Pedophile Internet Message Board

    Science.gov (United States)

    Malesky, L. Alvin, Jr.; Ennis, Liam

    2004-01-01

    A covert observation of posts on a pro-pedophile Internet message board investigated evidence of distorted cognitions that were supportive of sexually abusive behavior. Implications for the treatment and supervision of members of online communities that support pedophilic interests and behaviors are discussed. The purpose of the present study was…

  13. ORNL ADCP POST-PROCESSING GUIDE AND MATLAB ALGORITHMS FOR MHK SITE FLOW AND TURBULENCE ANALYSIS

    Energy Technology Data Exchange (ETDEWEB)

    Gunawan, Budi [Oak Ridge National Laboratory (ORNL); Neary, Vincent S [ORNL

    2011-09-01

    Standard methods, along with guidance for post-processing the ADCP stationary measurements using MATLAB algorithms that were evaluated and tested by Oak Ridge National Laboratory (ORNL), are presented following an overview of the ADCP operating principles, deployment methods, error sources and recommended protocols for removing and replacing spurious data.

  14. Evaluation of an Innovative Post-Arrest Diversion Program: 12-Month Recidivism Analysis

    Science.gov (United States)

    Dembo, Richard; Walters, Wansley; Wareham, Jennifer; Burgos, Catherimarty; Schmeidler, James; Hoge, Robert; Underwood, Lee

    2008-01-01

    We report the results of an evaluation study of a diversion program, operated by the Miami-Dade County Juvenile Assessment Center in Florida. The Post-Arrest Diversion (PAD) program represents an innovative approach to treatment and intervention within the juvenile justice system that utilizes standardized psychosocial risks and needs assessment…

  15. The Overlap between Emotional Intelligence and Post-Industrial Leadership Capacity: A Construct Validity Analysis

    Science.gov (United States)

    Rosch, David M.; Joseph, Dana L.; Newman, Daniel A.

    2011-01-01

    A sample of 276 students enrolled in campus leadership programs completed the Emotional Competence Inventory-University Edition (ECI-U) and the Socially Responsible Leadership Scale (SRLS) as a means to determine the relatedness in college students of emotional intelligence (EI) to the practice of post-industrial leadership skills. Confirmatory…

  16. Glycoproteomic Analysis of Seven Major Allergenic Proteins Reveals Novel Post-translational Modifications*

    OpenAIRE

    Halim, Adnan; Carlsson, Michael C.; Madsen, Caroline Benedicte; Brand, Stephanie; Møller, Svenning Rune; Olsen, Carl Erik; Vakhrushev, Sergey Y.; Brimnes, Jens; Wurtzen, Peter Adler; Ipsen, Henrik; Petersen, Bent L.; Wandall, Hans H.

    2014-01-01

    Allergenic proteins such as grass pollen and house dust mite (HDM) proteins are known to trigger hypersensitivity reactions of the immune system, leading to what is commonly known as allergy. Key allergenic proteins including sequence variants have been identified but characterization of their post-translational modifications (PTMs) is still limited.

  17. Systems Analysis of Interaction between Russia and the European Union in the Post-Soviet Space

    Directory of Open Access Journals (Sweden)

    Dmitry Vasfilov

    2014-05-01

    Full Text Available This article analyzes post-Soviet relations between Russia and the European Union using the theoretical framework of neoclassical realism. It finds that the post-Soviet level of competition between Russia and the EU is higher than required by the international system. The reason is rooted in the influence of a number of internal factors (or intervening variables. Consequently, elites in both Russia and the EU are not able to adequately understand the signals sent by the international system. There is a wide variety of intervening variables; for example, there are factors caused by the political elites’ perceptions of each other’s intentions and of the international situation, factors related to inadequate information, factors related to the complex institutional structure of the EU and factors related to domestic political issues. In addition, the current international environment, characterized by a high degree of uncertainty, increases the effects of these intervening variables. These effects result in inaccurate and incorrect processing of the signals of the international system by Russian and European elites. As a result, a subsystem of international relations has arisen in the post-Soviet space, featuring a highly competitive environment. However, there are only two major actors in the region: Russia and the EU. Small countries are too weak, so must choose to align themselves with either Russia or the Euone or the other. This causes a rivalry between Russia and the EU for influence on small and medium-sized countries in the post-Soviet space.

  18. Exploration of genetically encoded voltage indicators based on a chimeric voltage sensing domain

    Directory of Open Access Journals (Sweden)

    Yukiko Mishina

    2014-09-01

    We recently introduced a new VSFP design in which the voltage-sensing domain (VSD is sandwiched between a FRET pair of fluorescent proteins (termed VSFP-Butterflies and also demonstrated a series of chimeric VSD in which portions of the VSD of Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP are substituted by homologous portions of a voltage-gated potassium channel subunit. These chimeric VSD had faster sensing kinetics than that of the native Ci-VSD. Here, we describe a new set of VSFPs that combine chimeric VSD with the Butterfly structure. We show that these chimeric VSFP-Butterflies can report membrane voltage oscillations of up to 200 Hz in cultured cells and report sensory evoked cortical population responses in living mice. This class of GEVIs may be suitable for imaging of brain rhythms in behaving mammalians.

  19. A chimeric measles virus with a lentiviral envelope replicates exclusively in CD4+/CCR5+ cells

    International Nuclear Information System (INIS)

    We generated a replicating chimeric measles virus in which the hemagglutinin and fusion surface glycoproteins were replaced with the gp160 envelope glycoprotein of simian immunodeficiency virus (SIVmac239). Based on a previously cloned live-attenuated Schwarz vaccine strain of measles virus (MV), this chimera was rescued at high titers using reverse genetics in CD4+ target cells. Cytopathic effect consisted in the presence of large cell aggregates evolving to form syncytia, as observed during SIV infection. The morphology of the chimeric virus was identical to that of the parent MV particles. The presence of SIV gp160 as the only envelope protein on chimeric particles surface altered the cell tropism of the new virus from CD46+ to CD4+ cells. Used as an HIV candidate vaccine, this MV/SIVenv chimeric virus would mimic transient HIV-like infection, benefiting both from HIV-like tropism and the capacity of MV to replicate in dendritic cells, macrophages and lymphocytes.

  20. Spatial analysis and site selection post centers using Geographical Information System (Case study: zone 5 & 6 of Isfahan city

    Directory of Open Access Journals (Sweden)

    H. Kiumarsi

    2012-01-01

    Full Text Available Extended abstract1- Introduction In recent decades, rapid growth of urban population,rural-urban migrations & urban regions natural population growth, made very difficults for big cities & metropolises of our country. Before of this time Cities that had little growth, with very new difficults faced. One of the Maine needs of urban populations is fine access to urban sevices. Accabilitiy is one of the Maine characterizes of a good city and can divide to diver shape of access such as activities, goods, resources and services.2- MethodologyPrimary, Effective alternatives of post center site selection converted for information layer with shape file format.In next stage, best buffer for any layers determined and for each considered range weigth between 1(lowest value & 5(higthest value. Because the entire information layer that used isn't equall effect in post center site selection and because to be many Layers, compare & determine value of each seems very difficult, so AHP technique used for weigthing and combaine layers. The export of this stage is land zoning for locating post centers in 5 ranges with amount of goodness: very much, much, mediane, low & very low. So, in order to determining poor regions, network analysis tools in GIS environment used and 750 meters of standard buffer access for exist centers designed and with period rank planning, the best sites for construction post centers in 3 periods(low, mediane & long determined.3- DiscussionIn case study (zone 5 & 6 of Isfahan city, that 5 post services center exist, bad spatial distribution & locating near of the maine roads, created very difficults for settelers for access to this services. So in this research primary effective factor in post center site selection distincted that involved information layer such as radiant access, access to roads, nearest to attractive centers such as: educations, military, cultural, religious centers, center of regions and far from exists post centers. So

  1. Comparative classification analysis of post-harvest growth detection from terrestrial LiDAR point clouds in precision agriculture

    Science.gov (United States)

    Koenig, Kristina; Höfle, Bernhard; Hämmerle, Martin; Jarmer, Thomas; Siegmann, Bastian; Lilienthal, Holger

    2015-06-01

    In precision agriculture, detailed geoinformation on plant and soil properties plays an important role, e.g., in crop protection or the application of fertilizers. This paper presents a comparative classification analysis for post-harvest growth detection using geometric and radiometric point cloud features of terrestrial laser scanning (TLS) data, considering the local neighborhood of each point. Radiometric correction of the TLS data was performed via an empirical range-correction function derived from a field experiment. Thereafter, the corrected amplitude and local elevation features were explored regarding their importance for classification. For the comparison, tree induction, Naive Bayes, and k-Means-derived classifiers were tested for different point densities to distinguish between ground and post-harvest growth. The classification performance was validated against highly detailed RGB reference images and the red edge normalized difference vegetation index (NDVI705), derived from a hyperspectral sensor. Using both geometric and radiometric features, we achieved a precision of 99% with the tree induction. Compared to the reference image classification, the calculated post-harvest growth coverage map reached an accuracy of 80%. RGB and LiDAR-derived coverage showed a polynomial correlation to NDVI705 of degree two with R2 of 0.8 and 0.7, respectively. Larger post-harvest growth patches (>10 × 10 cm) could already be detected by a point density of 2 pts./0.01 m2. The results indicate a high potential of radiometric and geometric LiDAR point cloud features for the identification of post-harvest growth using tree induction classification. The proposed technique can potentially be applied over larger areas using vehicle-mounted scanners.

  2. Chimeric hepatitis B virus core particles as probes for studying peptide-integrin interactions.

    OpenAIRE

    Chambers, M A; Dougan, G; Newman, J.; Brown, F.; Crowther, J.; Mould, A P; Humphries, M J; Francis, M. J.; Clarke, B.; Brown, A L; Rowlands, D.

    1996-01-01

    An RGD-containing epitope from the foot-and-mouth disease virus (FMDV) VP1 protein was inserted into the e1 loop of the hepatitis B virus core (HBc) protein. This chimeric protein was expressed at high levels in Escherichia coli and spontaneously assembled into virus-like particles which could be readily purified. These fusion particles elicited high levels of both enzyme-linked immunosorbent assay- and FMDV-neutralizing antibodies in guinea pigs. The chimeric particles bound specifically to ...

  3. Assembling Single-Cell Genomes and Mini-Metagenomes From Chimeric MDA Products

    OpenAIRE

    Nurk, Sergey; Bankevich, Anton; Antipov, Dmitry; Gurevich, Alexey A.; Korobeynikov, Anton; Lapidus, Alla; Prjibelski, Andrey D.; Pyshkin, Alexey; Sirotkin, Alexander; Sirotkin, Yakov; Stepanauskas, Ramunas; Clingenpeel, Scott R.; Woyke, Tanja; Jeffrey S. McLean; Lasken, Roger

    2013-01-01

    Recent advances in single-cell genomics provide an alternative to largely gene-centric metagenomics studies, enabling whole-genome sequencing of uncultivated bacteria. However, single-cell assembly projects are challenging due to (i) the highly nonuniform read coverage and (ii) a greatly elevated number of chimeric reads and read pairs. While recently developed single-cell assemblers have addressed the former challenge, methods for assembling highly chimeric reads remain poorly explored. We p...

  4. Hematopoietic Chimerism Monitoring Based on STRs: Quantitative Platform Performance on Sequential Samples

    OpenAIRE

    Kristt, Don; Israeli, Moshe; Narinski, Ronit; Or, Hagit; Yaniv, I; Stein, Jerry; Klein, Tirza

    2005-01-01

    Hematopoietic stem cell transplantation (HSCT) creates a donor-recipient cellular chimerism in the patient, which is quantitatively assayed from peripheral blood based on STR-DNA. Since chimerism values often vary across a patient’s samples, it is important to determine to what extent this variability reflects technical aspects of platform performance. This issue is systematically assessed in the current study for the first time. Using the SGM Plus multiplex PCR kit and ABI platform, the long...

  5. Context Dependent Effects of Chimeric Peptide Morpholino Conjugates Contribute to Dystrophin Exon-skipping Efficiency

    OpenAIRE

    Yin, HaiFang; Boisguerin, Prisca; Moulton, Hong M.; Betts, Corinne; Seow, Yiqi; Boutilier, Jordan; Wang, Qingsong; Walsh, Anthony; Lebleu, Bernard; Wood, Matthew JA

    2013-01-01

    We have recently reported that cell-penetrating peptides (CPPs) and novel chimeric peptides containing CPP (referred as B peptide) and muscle-targeting peptide (referred as MSP) motifs significantly improve the systemic exon-skipping activity of morpholino phosphorodiamidate oligomers (PMOs) in dystrophin-deficient mdx mice. In the present study, the general mechanistic significance of the chimeric peptide configuration on the activity and tissue uptake of peptide conjugated PMOs in vivo was ...

  6. Targeting duplex DNA with chimeric α,β-triplex-forming oligonucleotides

    OpenAIRE

    Kolganova, N. A.; Shchyolkina, A K; Chudinov, A. V.; Zasedatelev, A S; Florentiev, V L; Timofeev, E. N.

    2012-01-01

    Triplex-directed DNA recognition is strictly limited by polypurine sequences. In an attempt to address this problem with synthetic biology tools, we designed a panel of short chimeric α,β-triplex-forming oligonucleotides (TFOs) and studied their interaction with fluorescently labelled duplex hairpins using various techniques. The hybridization of hairpin with an array of chimeric probes suggests that recognition of double-stranded DNA follows complicated rules combining reversed Hoogsteen and...

  7. Post-vehicle-application lithium-ion battery remanufacturing, repurposing and recycling capacity: Modeling and analysis

    Directory of Open Access Journals (Sweden)

    Charles Robert Standridge

    2015-05-01

    Full Text Available Purpose: A mathematical model is used to help determine the manufacturing capacity needed to support post-vehicle-application remanufacturing, repurposing, and recycling of lithium-ion batteries over time.  Simulation is used in solving the model to estimate capacity in kWh.  Lithium-ion batteries that are commonly used in the electrification of vehicles cannot be simply discarded post-vehicle-application due to the materials of which they are composed.  Eventually, each will fail to hold a charge and will need to be recycled.  Remanufacturing, allowing a battery to return to a vehicle application, and repurposing, transforming a battery for use in a non-vehicle application, postpone recycling and increase value. The mathematical model and its solution using simulation test the hypothesis that the capacity needed for remanufacturing, repurposing, and recycling as well as new battery production is a function of a single parameter:  the percent of post-vehicle-application batteries that are remanufactured. Design/methodology/approach: Equations in the mathematical model represent the capacity needed for remanufacturing, repurposing, and recycling as well as new battery production as dependent variables.  Independent variables are exogenous quantities as such as the demand for electrified vehicles of all types, physical properties of batteries such as their application life distribution including the time to recycling, and a single decision variable:  the percent of post-vehicle-application batteries that are remanufactured.  Values of the dependent variables over time are estimated by simulation for values of the percent of post-vehicle-application batteries ranging from 0% to 85% in steps of 5%. Findings and Originality/value: The simulation results support important insights for investment in capacity for remanufacturing, repurposing, and recycling of post-vehicle-application batteries as well as new batteries.  The capacity needed for

  8. Stochastic analysis of laminated composite plates on elastic foundation: The cases of post-buckling behavior and nonlinear free vibration

    Energy Technology Data Exchange (ETDEWEB)

    Singh, B.N., E-mail: bnsingh@aero.iitkgp.ernet.i [Department of Aerospace Engineering, IIT Kharagpur 721 302, West Bengal (India); Lal, Achchhe [Department of Mechanical Engineering, SVNIT, Surat 395007 (India)

    2010-10-15

    This study deals with the stochastic post-buckling and nonlinear free vibration analysis of a laminated composite plate resting on a two parameters Pasternak foundation with Winkler cubic nonlinearity having uncertain system properties. The system properties are modeled as basic random variables. A C{sup 0} nonlinear finite element formulation of the random problem based on higher-order shear deformation theory in the von Karman sense is presented. A direct iterative method in conjunction with a stochastic nonlinear finite element method proposed earlier by the authors is extended to analyze the effect of uncertainty in system properties on the post-buckling and nonlinear free vibration of the composite plates having Winler type of geometric nonlinearity. Mean as well as standard deviation of the responses have been obtained for various combinations of geometric parameters, foundation parameters, stacking sequences and boundary conditions and compared with those available in the literature and Monte Carlo simulation.

  9. Work-related critical incidents in hospital-based health care providers and the risk of post-traumatic stress symptoms, anxiety, and depression: A meta-analysis

    NARCIS (Netherlands)

    J.C. Boer; A. Lok; E. van't Verlaat; H.J. Duivenvoorden; A.B. Bakker; B.J. Smit

    2011-01-01

    This meta-analysis reviewed existing data on the impact of work-related critical incidents in hospital-based health care professionals. Work-related critical incidents may induce post-traumatic stress symptoms or even post-traumatic stress disorder (FTSD), anxiety, and depression and may negatively

  10. Mosaic origins of a complex chimeric mitochondrial gene in Silene vulgaris.

    Directory of Open Access Journals (Sweden)

    Helena Storchova

    Full Text Available Chimeric genes are significant sources of evolutionary innovation that are normally created when portions of two or more protein coding regions fuse to form a new open reading frame. In plant mitochondria astonishingly high numbers of different novel chimeric genes have been reported, where they are generated through processes of rearrangement and recombination. Nonetheless, because most studies do not find or report nucleotide variation within the same chimeric gene, evolution after the origination of these chimeric genes remains unstudied. Here we identify two alleles of a complex chimera in Silene vulgaris that are divergent in nucleotide sequence, genomic position relative to other mitochondrial genes, and expression patterns. Structural patterns suggest a history partially influenced by gene conversion between the chimeric gene and functional copies of subunit 1 of the mitochondrial ATP synthase gene (atp1. We identified small repeat structures within the chimeras that are likely recombination sites allowing generation of the chimera. These results establish the potential for chimeric gene divergence in different plant mitochondrial lineages within the same species. This result contrasts with the absence of diversity within mitochondrial chimeras found in crop species.

  11. Targeting duplex DNA with chimeric α,β-triplex-forming oligonucleotides.

    Science.gov (United States)

    Kolganova, N A; Shchyolkina, A K; Chudinov, A V; Zasedatelev, A S; Florentiev, V L; Timofeev, E N

    2012-09-01

    Triplex-directed DNA recognition is strictly limited by polypurine sequences. In an attempt to address this problem with synthetic biology tools, we designed a panel of short chimeric α,β-triplex-forming oligonucleotides (TFOs) and studied their interaction with fluorescently labelled duplex hairpins using various techniques. The hybridization of hairpin with an array of chimeric probes suggests that recognition of double-stranded DNA follows complicated rules combining reversed Hoogsteen and non-canonical homologous hydrogen bonding. In the presence of magnesium ions, chimeric TFOs are able to form highly stable α,β-triplexes, as indicated by native gel-electrophoresis, on-array thermal denaturation and fluorescence-quenching experiments. CD spectra of chimeric triplexes exhibited features typically observed for anti-parallel purine triplexes with a GA or GT third strand. The high potential of chimeric α,β-TFOs in targeting double-stranded DNA was demonstrated in the EcoRI endonuclease protection assay. In this paper, we report, for the first time, the recognition of base pair inversions in a duplex by chimeric TFOs containing α-thymidine and α-deoxyguanosine. PMID:22641847

  12. Post-irradiation data analysis for NRC/PNL Halden assembly IFA-431

    International Nuclear Information System (INIS)

    Results are presented for the post irradiation examination performed on IFA-431, which was a 6-rod test fuel assembly irradiated in Halden Reactor, Norway, under sponsorship of the Nuclear Regulatory Commission. The irradiation conditions included: peak powers of 33 kW/m; coolant pressure and temperature of 3.3 MPa and 2400C, respectively; and peak burnup of 4300 MWd/MTM. IFA-431 included instrumented rods of basic boiling water reactor design, with variations in fill gas composition, gap size, and UO2 fuel type. The irradiation was designed to measure the effect of these variations upon fuel rod thermal and mechanical performance. The post irradiation examination assessed the permanent changes to the rods, including induced radioactivity, cladding deformation, fission gas release, and fuel densification

  13. ANALYSIS FOR MARKETING STRATEGY PLAN OF NANCHANG CITY CHINA POST LOGISTICS COMPANY IN OTC-MEDICINAL MARKET : Study Case: Nanchang City China Post Logistics co.ltd (NCCNPL)

    OpenAIRE

    Wei, Qin

    2012-01-01

    Nanchang City, China Post Logistics Co ltd (NCCNPL) is a subsidiary of China post group, located in Jiangxi Province. The company established in January 18, 2003. NCCNPL is stated-owned enterprise logistics business collected storage, packaging, distribution, processing, cargo handling, transport and information services in a modern intergraded logistics enterprise. It is a professional logistics company that covers nationwide. The thesis idea is clearly to analyse the marketing strategy plan...

  14. Post-collisional deformation of the Anatolides and motion of the Arabian indenter: A paleomagnetic analysis

    Energy Technology Data Exchange (ETDEWEB)

    Piper, J [Geomagnetism Laboratory, Department of Earth and Ocean Sciences, University of Liverpool, Liverpool, L69 7ZE (United Kingdom); Tatar, O; Gursoy, H; Mesci, B L; Kocbulut, F [Department of Geology, Cumhuriyet University, 58140 Sivas (Turkey); Huang, B [Palaeomagnetism and Geochronology Laboratory, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing (China)], E-mail: sg04@liverpool.ac.uk

    2008-07-01

    In the Anatolides of Turkey the neotectonic (post collisional) phase of deformation embraces the period since final closure of the southern arm of Neotethys in mid-Miocene times. The Arabian Shield indenter has continued to deform into the weak Anatolian accretionary collage resulting from subduction of this ocean by a combination of differential movement relative to the African Plate and counterclockwise (CCW) rotation. Much of resulting deformation has been accommodated by slip along major transforms comprising the North Anatolian Fault Zone (NAFZ), the East Anatolian Fault Zone (EAFZ) and the northward extension of the Dead Sea Fault Zone (DSFZ) but has also been distributed as differential block rotations through the zone of weak crust in between. Facets of this deformation comprise crustal thickening and uplift to produce the Anatolian Plateau, establishment of transform faults and tectonic escape as Arabia has continued to impinge into the Anatolian collage. Paleomagnetic analysis of this deformation is facilitated by the widespread distribution of neotectonic volcanism and graben infills, and rotations relative to the Eurasian reference frame are recognised on two scales. Rapid rotation (up to 5{sup 0}/10,000 years) of small fault blocks is identified between master faults along the intracontinental transforms but deformation does not extend away from these zones and shows that seismogenic upper crust is decoupled from a lower continental lithosphere undergoing continuum deformation. The broad area of weak accreted crust between the transforms is dissected into large fault blocks which exhibit much lower rotation rates (mostly < 1{sup 0}/100,000 years) that vary systematically across the Anatolides. Large CCW rotations near the Arabian indenter diminish westwards to become zero then CW near the limit of tectonic escape in western Turkey. The view that the collage has rotated anticlockwise as a single plate, either uniformly or episodically, during the

  15. Post-collisional deformation of the Anatolides and motion of the Arabian indenter: A paleomagnetic analysis

    International Nuclear Information System (INIS)

    In the Anatolides of Turkey the neotectonic (post collisional) phase of deformation embraces the period since final closure of the southern arm of Neotethys in mid-Miocene times. The Arabian Shield indenter has continued to deform into the weak Anatolian accretionary collage resulting from subduction of this ocean by a combination of differential movement relative to the African Plate and counterclockwise (CCW) rotation. Much of resulting deformation has been accommodated by slip along major transforms comprising the North Anatolian Fault Zone (NAFZ), the East Anatolian Fault Zone (EAFZ) and the northward extension of the Dead Sea Fault Zone (DSFZ) but has also been distributed as differential block rotations through the zone of weak crust in between. Facets of this deformation comprise crustal thickening and uplift to produce the Anatolian Plateau, establishment of transform faults and tectonic escape as Arabia has continued to impinge into the Anatolian collage. Paleomagnetic analysis of this deformation is facilitated by the widespread distribution of neotectonic volcanism and graben infills, and rotations relative to the Eurasian reference frame are recognised on two scales. Rapid rotation (up to 50/10,000 years) of small fault blocks is identified between master faults along the intracontinental transforms but deformation does not extend away from these zones and shows that seismogenic upper crust is decoupled from a lower continental lithosphere undergoing continuum deformation. The broad area of weak accreted crust between the transforms is dissected into large fault blocks which exhibit much lower rotation rates (mostly 0/100,000 years) that vary systematically across the Anatolides. Large CCW rotations near the Arabian indenter diminish westwards to become zero then CW near the limit of tectonic escape in western Turkey. The view that the collage has rotated anticlockwise as a single plate, either uniformly or episodically, during the Neotectonic era is

  16. The need for complementary hydraulic analysis in post-restoration monitoring of river restoration projects

    OpenAIRE

    T. A. Endreny; M. M. Soulman

    2011-01-01

    River restoration design methods are incrementally improved by studying and learning from monitoring data in previous projects. In this paper, we report post-restoration monitoring data for a Natural Channel Design (NCD) restoration project along 1600 m (10 channel wavelengths) of the Batavia Kill in the Catskill Mountains, NY, implemented in 2001 and 2002. The NCD project used a reference-reach to determine channel form, empirical relations between the project site and reference site bankful...

  17. THE SCHUMPETERIAN STRUCTURAL ADJUSTMENT OF THE BANKING INDUSTRY: A POST FINANCIAL CRISIS ANALYSIS

    OpenAIRE

    JARUNEE WONGLIMPIYARAT; RACHANEE TRIPIPATKUL

    2005-01-01

    This paper discusses the economic growth and technological change of the Thai banking industry in relation to a post financial crisis, based on Schumpeter's economic development theory. It is argued that the structural changes of the Thai banking industry reflect Schumpeter's gales of creative destruction. The circumstance in which Thailand has to let the ailing banks and financial institutions go bankrupt and renew the process of growth through mergers and acquisitions represents an adjustme...

  18. Post-Apartheid Trends in Gender Discrimination in South Africa: Analysis through Decomposition Techniques

    OpenAIRE

    Debra Shepherd

    2008-01-01

    Using appropriate econometric methods and 11 representative household surveys, this paper empirically assesses the extent and evolution of gender discrimination in the South African labour market over the post-apartheid period. Attention is also paid to the role that anti-discriminatory legislation has had to play in effecting change in the South African labour market. Much of the paper’s focus is placed on African women who would have benefited most from the new legislative environment. Afri...

  19. Paul Davidson, Post Keynesian Theory and Policy: A Realistic Analysis of the Market Oriented Capitalist Economy

    OpenAIRE

    Voss, Stefan

    2016-01-01

    Abstract. In this book Davidson develops Post Keynesian strategies for full employment policies, targeting inflation and offers solutions for the problems of globalization. Davidson´s message is clear: Markets cannot regulate by itself. Mainstream theories are all based on wrong assumptions like the neutrality of money, the predictability of the future (ergodic-axiom), the efficiency of markets, rational expectations, Say´s Law and Walrasian Theory of equilibriums. In this book Davidson rejec...

  20. An Analysis of Central Bank Interventions on Forex Market For The Post-Crisis Period

    OpenAIRE

    Korap, Levent

    2006-01-01

    In this paper, we investigate the determinants of foreign exchange operations of the CBRT for the post-crisis period. Using modern time series econometrics, we try to analyze the different characteristics of FOREX market, and based on the estimation results, indicate the degree of effectiveness of the interventions of monetary authority throughout the implicit inflation targeting framework. The main policy conclusion is that the CBRT interventions seem to be under the control of uncertainties...

  1. The application of the initial post-buckling analysis to shells

    International Nuclear Information System (INIS)

    The energy approach to elastic stability is employed to describe characteristic differences in buckling behaviour between bars in compression, plates loaded in their plane, and shells, in particular in the absence of inextensional deformation. The initial stage of post-buckling behaviour of shells is discussed from the stand-point of shallow shell theory and it is applied to more or less localized buckling patterns. (orig.)

  2. Post-reform Trends in Wage-Differentials: A Decomposition Analysis for India

    OpenAIRE

    Mukherjee, Dipa

    2007-01-01

    Wage inequality often creates much broader socio-economic inequality and may even accentuate them. For attaining equitable development convergence in wages and earnings is therefore desirable. This paper explores trends and patterns in wage differentials in India in the post reform period. Using decomposition technique it compares trends in within group and between group disparities – across occupational group, gender, job type, and region. It is observed that while inter-group disparity or v...

  3. Smoking cessation and inattention or hyperactivity/impulsivity: a post hoc analysis

    OpenAIRE

    Covey, Lirio S.; Manubay, Jeanne; Jiang, Huiping; Nortick, Mariel; Palumbo, Domenica

    2008-01-01

    Tobacco use is more prevalent and smoking cessation less likely among persons with attention deficit hyperactivity disorder (ADHD) than the general population. Evidence that tobacco use and nicotine hold divergent relationships with inattention (IN) and hyperactivity/impulsivity (HI), the core symptoms of ADHD, prompted this post hoc investigation of abstinence patterns by type of ADHD symptoms. Subjects were 583 adult smokers treated openly with bupropion and nicotine patch during the initia...

  4. POST PARTUM PSYCHIATRIC SYNDROMES—AN ANALYSIS OF 100 CONSECUTIVE CASES1

    OpenAIRE

    Gautam, Shiv; Nijhawan, Madhu; Gehlot, P.S.

    1982-01-01

    SUMMARY 100 consecutive out patients with onset of Psychiatric illness within 40 days of child birth were studied by using Indian Psychiatric Interview Schedule and a specially designed Proforma. It was found that post partum psychiatric syndromes occurred most frequently in patients less than 25 years of age. Stress of Physical illness was present in a considerably high number of patients (74%). In 66% the Psychiatric illness was associated with first child birth and risk of Puerperal Psycho...

  5. Reproductive history and post-reproductive mortality: A sibling comparison analysis using Swedish register data.

    Science.gov (United States)

    Barclay, Kieron; Keenan, Katherine; Grundy, Emily; Kolk, Martin; Myrskylä, Mikko

    2016-04-01

    A growing body of evidence suggests that reproductive history influences post-reproductive mortality. A potential explanation for this association is confounding by socioeconomic status in the family of origin, as socioeconomic status is related to both fertility behaviours and to long-term health. We examine the relationship between age at first birth, completed parity, and post-reproductive mortality and address the potential confounding role of family of origin. We use Swedish population register data for men and women born 1932-1960, and examine both all-cause and cause-specific mortality. The contributions of our study are the use of a sibling comparison design that minimizes residual confounding from shared family background characteristics and assessment of cause-specific mortality that can shed light on the mechanisms linking reproductive history to mortality. Our results were entirely consistent with previous research on this topic, with teenage first time parents having higher mortality, and the relationship between parity and mortality following a U-shaped pattern where childless men and women and those with five or more children had the highest mortality. These results indicate that selection into specific fertility behaviours based upon socioeconomic status and experiences within the family of origin does not explain the relationship between reproductive history and post-reproductive mortality. Additional analyses where we adjust for other lifecourse factors such as educational attainment, attained socioeconomic status, and post-reproductive marital history do not change the results. Our results add an important new level of robustness to the findings on reproductive history and mortality by showing that the association is robust to confounding by factors shared by siblings. However it is still uncertain whether reproductive history causally influences health, or whether other confounding factors such as childhood health or risk-taking propensity could

  6. Post-Merger Share Price Performance Analysis: A Case Study of UBA Group Plc.

    OpenAIRE

    Ogundipe, Oyekunbi

    2009-01-01

    Abstract. Mergers have been taught of as a form of corporate development and most organisations use it as a route to achieving economies of scale and scope amongst others. This piece of work sheds light on a government induced merger that took place in the Nigerian banking sector and tries to examine how the share price has performed post merger. We conclude that a country initiated merger is not the ultimate form of corporate development but rather a market induced merger w...

  7. ANALYSIS OF 57 POST - ENDOPHTHALMITIS R ETINAL D ETACHMENT AND PREDI CTIVE FACTORS FOR VISUAL OUTCOME

    Directory of Open Access Journals (Sweden)

    Vivek

    2015-05-01

    Full Text Available OBJECTIVE: Evaluation of cases of endophthalmitis developing Rhegmatogenous retinal detachment and managed with vitreoretinal surgery , their clinical profile , factors responsible for retinal detachment and the predictive factors for the final outcome. SETTING: Vivekanand Netralaya , Lions NAB Eye hospital , Miraj . MATERIAL AND METHODS: This is a retrospective nonrandomized case series involving 57 consecutive cases of post endophthalmitis Rhegmatogenous R etinal Detachment. 168 cases of e ndophthalmitis that were managed were reviewed. Amongst these cases 57 developed rhegmatogrnous RD at various stages of presentation and management. All these cases were managed by Parsplana vitrectomy with F luid G gas E xcha nge with endolaser and silicon oil /C3F8 implantation. RESULTS: 168 cases of endophthalmitis ( 90 - posttraumatic/ 72 - post - operative/ 6 endogenous , were managed of which 33.92 % developed Retinal Detachment. Of the patients with RD 24.6% it was noted post vitr ectomy surgery for endophthalmitis in the rest it was noted pre or intraoperative. CONCLUSION : F actors compatible with good visual outcome were macula on RD (68% , PVD induction (56% , and also the cases with silicon oil tamponade , breaks and retinal detachment noted i ntroperatively ( D uring vitrectomy for endophthalmitis fared much better . With better surgical techniques , availability of antimicrobials , long acting ta mponading agent s the v itreoretinal surgical results are encouragin g , can be even better if intervened early.

  8. A preliminary structural analysis of the pattern of post-glacial faults in Northern Sweden

    International Nuclear Information System (INIS)

    The post-glacial kinematics inferred from the structures suggest horizontal NW or WNW compression with lateral relief to the NE at depth below surficial upward relief. These kinematics are sufficiently similar to the kinematics throughout Europe north of the Alps to suspect the neotectonic motion of the Eurasian lithospheric plate over the last 38 or even 58 (+/- 2)Ma as the causative force. Large displacements appear to have occurred within a hundred years or so of the last ice retreating from any particular part of northern Scandinavia. This suggests a sudden relief during glacial unloading (and melting of ground water) of plate tectonic forces accumulated during glacial loading. Recent earthquakes in the region display similar kinematics to the post-glacial fractures on a regional scale but indications of ground movement along the forested fault scarps in the lifetime of the trees is observed at only a few localities. Only geodetic strain measurements or small-earthquake monitoring will resolve whether current ductile or seismic motion is occuring along the most obvious post-glacial scarps. Such investigations are under way. (orig./DG)

  9. Transition to Democracy in Post-Soviet States: Success or Failure. Case Study Analysis.

    Directory of Open Access Journals (Sweden)

    Ceyhun Valiyev

    2012-07-01

    Full Text Available The democratization of Post-Soviet states in past two decades is the subject of this academic study. The main question of this research is that why most of the Post-Soviet states haven’t gone through successful transition to democracy. Five countries; Azerbaijan, Belorussia, Georgia, Lithuania and Uzbekistan are the casesof this study to analyze and evaluate as empirical part of this work. I haven’t chosen the countries that have standard and equal level of success or failure. For instance, Lithuania is among those countries that have gone through quite successful process of democratization, whereas others have similar or different problems hindering the full-fledged democratization across the Post-Soviet area. Insome other cases, such as in Georgia the transition has not been fully successful but some measures of democracy are considered to be existent in state institutions and society. Several hypotheses have been developed throughout this study all arguing the preconditions that lead to democratization, then these hypotheses are checked ifthey are applicable in the cases used in this study. The conclusion is that not all these hypotheses are correct in every single case and each case study has its own characteristic causes that lead to failure or success in transition to democracy.

  10. POST-CASKETSS: a graphic computer program for thermal and structural analysis of nuclear fuel shipping casks

    International Nuclear Information System (INIS)

    A computer program POST-CASKETSS has been developed for the purpose of calculation result representation for thermal and structural analysis computer code system CASKETSS (CASKETSS means a modular code system for CASK Evaluation code system for Thermal and Structural Safety). Main features of POST-CASKETSS are as follows; (1) Function of calculation result representation for thermal and structural analysis computer programs is provided in the program. (2) Two and three dimensional graphic representation for finite element and finite difference programs are available in the program. (3) The capacity of graphics of geometry, temperature contor and temperature-time curve are provided for thermal analysis. (4) The capacity of graphics of geometry, deformation, stress contor, displacement-time curve, velocity-time curve, acceleration-time curve, stress-time curve, force-time curve and moment-time curve are provided for structural analysis. (5) This computer program operates both the time shearing system and the batch system. In the paper, brief illustration of calculation method, input data and sample calculations are presented. (author)

  11. RT-SHIV, an infectious CCR5-tropic chimeric virus suitable for evaluating HIV reverse transcriptase inhibitors in macaque models

    Directory of Open Access Journals (Sweden)

    Emau Peter

    2009-11-01

    Full Text Available Abstract Background Non-nucleoside reverse transcriptase inhibitors (NNRTIs are an important category of drugs for both chemotherapy and prevention of human immunodeficiency virus type 1 (HIV-1 infection. However, current non-human primate (NHP models utilizing simian immunodeficiency virus (SIV or commonly used chimeric SHIV (SIV expressing HIV-1 envelope are inadequate due to the insensitivity to NNRTIs. To develop a NHP model for evaluation of NNRTI compounds, we characterized a RT-SHIV virus that was assembled by replacing the SIVmac239 reverse transcriptase (RT with that of HIV-1HXB2. Since RT-SHIV exhibited in vitro characteristics of high infectivity, CCR5-usage, and sensitivity to HIV-1 specific NNRTIs, this virus was thought to be suitable for mucosal transmission and then was used to carry out a vaginal transmission study in pigtail macaques (Macaca nemestrina. Results RT-SHIV exhibited in vitro characteristics of an infectious CCR5-tropic chimeric virus. This virus was not only highly sensitive to HIV-1 RT specific NNRTIs; its replication was also inhibited by a variety of NRTIs and protease inhibitors. For in vivo vaginal transmission studies, macaques were either pretreated with a single dose of DMPA (depot medroxyprogesterone acetate or left untreated before intravaginal inoculation with 500 or 1,000 TCID50 of RT-SHIV. All macaques became systemically infected by 2 or 3 weeks post-inoculation exhibiting persistent high viremia, marked CD4+T cell depletion, and antiviral antibody response. DMPA-pretreated macaques showed a higher mean plasma viral load after the acute infection stage, highly variable antiviral antibody response, and a higher incidence of AIDS-like disease as compared with macaques without DMPA pretreatment. Conclusion This chimeric RT-SHIV has exhibited productive replication in both macaque and human PBMCs, predominantly CCR5-coreceptor usage for viral entry, and sensitivity to NNRTIs as well as other anti

  12. Structural Characterization by NMR of a Double Phosphorylated Chimeric Peptide Vaccine for Treatment of Alzheimer’s Disease

    Directory of Open Access Journals (Sweden)

    Stefan Berger

    2013-04-01

    Full Text Available Rational design of peptide vaccines becomes important for the treatment of some diseases such as Alzheimer’s disease (AD and related disorders. In this study, as part of a larger effort to explore correlations of structure and activity, we attempt to characterize the doubly phosphorylated chimeric peptide vaccine targeting a hyperphosphorylated epitope of the Tau protein. The 28-mer linear chimeric peptide consists of the double phosphorylated B cell epitope Tau229-237[pThr231/pSer235] and the immunomodulatory T cell epitope Ag85B241-255 originating from the well-known antigen Ag85B of the Mycobacterium tuberculosis, linked by a four amino acid sequence -GPSL-. NMR chemical shift analysis of our construct demonstrated that the synthesized peptide is essentially unfolded with a tendency to form a β-turn due to the linker. In conclusion, the -GPSL- unit presumably connects the two parts of the vaccine without transferring any structural information from one part to the other. Therefore, the double phosphorylated epitope of the Tau peptide is flexible and accessible.

  13. Immunogenicity of IMS 1113 plus soluble subunit and chimeric proteins containing Mycoplasma hyopneumoniae P97 C-terminal repeat regions.

    Science.gov (United States)

    Barate, Abhijit K; Cho, Youngjae; Truong, Quang Lam; Hahn, Tae-Wook

    2014-03-01

    The surface adhesin P97 mediates the adherence of Mycoplasma hyopneumoniae to swine cilia. Two reiterated repeats R1 and R2 are located at the C-terminus of P97. The purpose of this study was to evaluate the immunogenicity of Montanide adjuvant IMS 1113 plus soluble subunit proteins rR1, rR1R2 and their chimeric forms coupled with B subunit of the heat-labile enterotoxin of Escherichia coli (LTB). Each recombinant protein in this study was capable of eliciting anti-R1 specific humoral antibodies (IgG), mucosal antibodies (IgG and IgA) and IFN-γ production. The chimeric protein rLTBR1R2 elicited the quickest humoral antibody response among the recombinant proteins. Serum and bronchoalveolar lavage analysis revealed that each recombinant protein was capable of inducing both Th1 and Th2 responses. Importantly, all of the proteins induced an anti-R1-specific Th2-biased response in both humoral and mucosal compartments, similar to the response observed in a natural infection or vaccination process. These observations indicate that rR1, rR1R2, rLTBR1 and rLTBR1R2 with IMS 1113 might represent a promising subunit vaccine strategy against porcine enzootic pneumonia in pigs. PMID:24461070

  14. Risk assessment of relapse by lineage-specific monitoring of chimerism in children undergoing allogeneic stem cell transplantation for acute lymphoblastic leukemia

    Science.gov (United States)

    Preuner, Sandra; Peters, Christina; Pötschger, Ulrike; Daxberger, Helga; Fritsch, Gerhard; Geyeregger, Rene; Schrauder, André; von Stackelberg, Arend; Schrappe, Martin; Bader, Peter; Ebell, Wolfram; Eckert, Cornelia; Lang, Peter; Sykora, Karl-Walter; Schrum, Johanna; Kremens, Bernhard; Ehlert, Karoline; Albert, Michael H.; Meisel, Roland; Lawitschka, Anita; Mann, Georg; Panzer-Grümayer, Renate; Güngör, Tayfun; Holter, Wolfgang; Strahm, Brigitte; Gruhn, Bernd; Schulz, Ansgar; Woessmann, Wilhelm; Lion, Thomas

    2016-01-01

    Allogeneic hematopoietic stem cell transplantation is required as rescue therapy in about 20% of pediatric patients with acute lymphoblastic leukemia. However, the relapse rates are considerable, and relapse confers a poor outcome. Early assessment of the risk of relapse is therefore of paramount importance for the development of appropriate measures. We used the EuroChimerism approach to investigate the potential impact of lineage-specific chimerism testing for relapse-risk analysis in 162 pediatric patients with acute lymphoblastic leukemia after allogeneic stem cell transplantation in a multicenter study based on standardized transplantation protocols. Within a median observation time of 4.5 years, relapses have occurred in 41/162 patients at a median of 0.6 years after transplantation (range, 0.13–5.7 years). Prospective screening at defined consecutive time points revealed that reappearance of recipient-derived cells within the CD34+ and CD8+ cell subsets display the most significant association with the occurrence of relapses with hazard ratios of 5.2 (P=0.003) and 2.8 (P=0.008), respectively. The appearance of recipient cells after a period of pure donor chimerism in the CD34+ and CD8+ leukocyte subsets revealed dynamics indicative of a significantly elevated risk of relapse or imminent disease recurrence. Assessment of chimerism within these lineages can therefore provide complementary information for further diagnostic and, potentially, therapeutic purposes aiming at the prevention of overt relapse. This study was registered at clinical.trials.gov with the number NC01423747. PMID:26869631

  15. Solution structure of a Plasmodium falciparum AMA-1/MSP 1 chimeric protein vaccine candidate (PfCP-2.9 for malaria

    Directory of Open Access Journals (Sweden)

    Jin Changwen

    2010-03-01

    Full Text Available Abstract Background The Plasmodium falciparum chimeric protein PfCP-2.9 is a promising asexual-stage malaria vaccine evaluated in clinical trials. This chimeric protein consists of two cysteine-rich domains: domain III of the apical membrane antigen 1 (AMA-1 [III] and the C-terminal region of the merozoite surface protein 1 (MSP1-19. It has been reported that the fusion of these two antigens enhanced their immunogenicity and antibody-mediated inhibition of parasite growth in vitro. Methods The 15N-labeled and 13C/15N-labeled PfCP-2.9 was produced in Pichia pastoris for nuclear magnetic resonance (NMR structure analysis. The chemical shift assignments of PfCP-2.9 were compared with those previously reported for the individual domains (i.e., PfAMA-1(III or PfMSP 1-19. The two-dimensional spectra and transverse relaxation rates (R2 of the PfMSP1-19 alone were compared with that of the PfCP-2.9. Results Confident backbone assignments were obtained for 122 out of 241 residues of PfCP-2.9. The assigned residues in PfCP-2.9 were very similar to those previously reported for the individual domains. The conformation of the PfMSP1-19 in different constructs is essentially the same. Comparison of transverse relaxation rates (R2 strongly suggests no weak interaction between the domains. Conclusions These data indicate that the fusion of AMA-1(III and MSP1-19 as chimeric protein did not change their structures, supporting the use of the chimeric protein as a potential malaria vaccine.

  16. On Baltic Slovenia and Adriatic Lithuania. A qualitative comparative analysis of patterns in post-communist transformation / Lars Fredrik Stöcker

    Index Scriptorium Estoniae

    Stöcker, Lars Fredrik, 1979-

    2014-01-01

    Arvustus: Norkus, Zenonas. On Baltic Slovenia and Adriatic Lithuania. A qualitative comparative analysis of patterns in post-communist transformation. Verlag Apostrofia Publishers, Central European University Press. Budapest und New York 2012

  17. Protective and immunological behavior of chimeric yellow fever dengue vaccine.

    Science.gov (United States)

    Halstead, Scott B; Russell, Philip K

    2016-03-29

    Clinical observations from the third year of the Sanofi Pasteur chimeric yellow fever dengue tetravalent vaccine (CYD) trials document both protection and vaccination-enhanced dengue disease among vaccine recipients. Children who were 5 years-old or younger when vaccinated experienced a DENV disease resulting in hospitalization at 5 times the rate of controls. On closer inspection, hospitalized cases among vaccinated seropositives, those at highest risk to hospitalized disease accompanying a dengue virus (DENV) infection, were greatly reduced by vaccination. But, seronegative individuals of all ages after being vaccinated were only modestly protected from mild to moderate disease throughout the entire observation period despite developing neutralizing antibodies at high rates. Applying a simple epidemiological model to the data, vaccinated seronegative individuals of all ages were at increased risk of developing hospitalized disease during a subsequent wild type DENV infection. The etiology of disease in placebo and vaccinated children resulting in hospitalization during a DENV infection, while clinically similar are of different origin. The implications of the observed mixture of DENV protection and enhanced disease in CYD vaccinees are discussed. PMID:26873054

  18. Toxicities of chimeric antigen receptor T cells: recognition and management.

    Science.gov (United States)

    Brudno, Jennifer N; Kochenderfer, James N

    2016-06-30

    Chimeric antigen receptor (CAR) T cells can produce durable remissions in hematologic malignancies that are not responsive to standard therapies. Yet the use of CAR T cells is limited by potentially severe toxicities. Early case reports of unexpected organ damage and deaths following CAR T-cell therapy first highlighted the possible dangers of this new treatment. CAR T cells can potentially damage normal tissues by specifically targeting a tumor-associated antigen that is also expressed on those tissues. Cytokine release syndrome (CRS), a systemic inflammatory response caused by cytokines released by infused CAR T cells can lead to widespread reversible organ dysfunction. CRS is the most common type of toxicity caused by CAR T cells. Neurologic toxicity due to CAR T cells might in some cases have a different pathophysiology than CRS and requires different management. Aggressive supportive care is necessary for all patients experiencing CAR T-cell toxicities, with early intervention for hypotension and treatment of concurrent infections being essential. Interleukin-6 receptor blockade with tocilizumab remains the mainstay pharmacologic therapy for CRS, though indications for administration vary among centers. Corticosteroids should be reserved for neurologic toxicities and CRS not responsive to tocilizumab. Pharmacologic management is complicated by the risk of immunosuppressive therapy abrogating the antimalignancy activity of the CAR T cells. This review describes the toxicities caused by CAR T cells and reviews the published approaches used to manage toxicities. We present guidelines for treating patients experiencing CRS and other adverse events following CAR T-cell therapy. PMID:27207799

  19. Dosimetry of chimeric TNT in lung tumor patients

    Institute of Scientific and Technical Information of China (English)

    CHEN Yangchun; CHEN Shaoliang; JU Dianwen; SHI Hongcheng; YAO Zhifeng

    2007-01-01

    The purpose of this study was to assess the absorbed dose of tumor and main critical organs in 131I labeled chimeric tumor necrotic treatment (chTNT). In 9 patients, a single intravenous dose of (29.6±3.7) MBq/kg was administered. Blood samples were drawn at different time intervals, and urine was collected for up to one week. Tissue distribution of 131I -chTNT was followed for up to one week by gamma camera imaging. Absorbed doses to the whole body and to normal organs were computed according to the MIRD scheme using Mirdose-3 software. S-factors for lung tumors were estimated by comparison with lungs of similar mass and position in the body. It was found that mean serum disappearance half time values for 131I-chTNT were (4.93±9.36) h and (61.7±21.2) h for α, β respectively,while that for whole body was(99±10) h. Mean urine biological clearance half time value was (90±10) h. The absorbed dose to tumor was (8.28±2.65) Gy, and the tumor-to-nontumor dose ratio was 3.95±1.55. And the mean effective dose to patients was (1.02±0.29) mSv/MBq.

  20. Development of chimeric antigen receptors for multiple myeloma.

    Science.gov (United States)

    Martínez-Cingolani, Carolina; Bories, Jean Christophe

    2016-04-15

    Multiple myeloma (MM) is a haematologic malignancy characterized by the expansion of monoclonal plasma cells in the bone marrow. It is associated with serum or urine monoclonal protein and organ damage including renal failure, anaemia, hypercalcaemia and bone lesions. Despite recent improvements MM still remains an incurable disease. Previous studies have shown that the adoptive transfer of autologous T-cells modified to express chimeric antigen receptors (CAR) is effective in cases of acute and chronic lymphoid leukaemia. However, the adjustment of CAR-T-cell therapy to MM is hindered by the scarcity of antigens specific to the tumour plasma cells. Most candidate targets are shared by healthy tissues, and entail high risks of toxicity. Therefore several strategies have been proposed to regulate CAR-T-cell function as well as to enhance CAR-T-cell specificity against tumour cells. In this article we summarize the surface markers that have been investigated as targets to eliminate MM plasma cells and the MM-specific CARs that have been developed to date. Then we describe the different CAR-T-cell designs that could be applied in the case of MM to circumvent current problems of toxicity. PMID:27068946

  1. Dosimetry of chimeric TNT in lung tumor patients

    International Nuclear Information System (INIS)

    The purpose of this study was to assess the absorbed dose of tumor and main critical organs in 131I labeled chimeric tumor necrotic treatment (chTNT). In 9 patients, a single intravenous dose of (29.6±3.7) MBq/kg was administered. Blood samples were drawn at different time intervals, and urine was collected for up to one week. Tissue distribution of 131I -chTNT was followed for up to one week by gamma camera imaging. Absorbed doses to the whole body and to normal organs were computed according to the MIRD scheme using Mirdose-3 software. S-factors for lung tumors were estimated by comparison with lungs of similar mass and position in the body. It was found that mean serum disappearance half time values for 131I-chTNT were (4.934±9.36) h and (61.74±21.2) h for α, β respectively, while that for whole body was(99±10) h. Mean urine biological clearance half time value was (90±10) h. The ab- sorbed dose to tumor was (8.28±2.65) Gy, and the tumor-to-nontumor dose ratio was 3.95±1.55. And the mean effective dose to patients was (1.02±0.29) mSv/MBq. (authors)

  2. Effects of pharmacological concentrations of dietary zinc oxide on growth of post-weaning pigs: a meta-analysis.

    Science.gov (United States)

    Sales, James

    2013-06-01

    Pharmacological dietary zinc (Zn) concentrations of 1,000 to 3,000 mg/kg diet from Zn oxide have been found to increase growth in post-weaning pigs. However, results were inconsistent among studies. A frequentist meta-analysis, in which effects were numerically described with standardized effect sizes (Hedges's g), was conducted in order to identify and quantify the responses in average daily gain (ADG), average daily feed intake (ADFI), and gain to feed ratio (G/F) in post-weaning pigs upon dietary Zn supplementation from Zn oxide. The inability of independent continuous variables to explain significant heterogeneity obtained with fixed effect models necessitated the use of random effects models to calculate summary statistics. Dietary Zn supplementation increased (P Zinc oxide provided a feasible alternative to in-feed antibiotics to improve growth in post-weaning pigs, and its reduction in diets due to potential environmental pollution will have to be negated by alternative feed additives and management strategies in order to prevent economic losses. PMID:23463368

  3. Genomic convergence analysis of schizophrenia: mRNA sequencing reveals altered synaptic vesicular transport in post-mortem cerebellum.

    Directory of Open Access Journals (Sweden)

    Joann Mudge

    Full Text Available Schizophrenia (SCZ is a common, disabling mental illness with high heritability but complex, poorly understood genetic etiology. As the first phase of a genomic convergence analysis of SCZ, we generated 16.7 billion nucleotides of short read, shotgun sequences of cDNA from post-mortem cerebellar cortices of 14 patients and six, matched controls. A rigorous analysis pipeline was developed for analysis of digital gene expression studies. Sequences aligned to approximately 33,200 transcripts in each sample, with average coverage of 450 reads per gene. Following adjustments for confounding clinical, sample and experimental sources of variation, 215 genes differed significantly in expression between cases and controls. Golgi apparatus, vesicular transport, membrane association, Zinc binding and regulation of transcription were over-represented among differentially expressed genes. Twenty three genes with altered expression and involvement in presynaptic vesicular transport, Golgi function and GABAergic neurotransmission define a unifying molecular hypothesis for dysfunction in cerebellar cortex in SCZ.

  4. Comparative proteomic analysis of histone post-translational modifications upon ischemia/reperfusion-induced retinal injury

    DEFF Research Database (Denmark)

    Zhao, Xiaolu; Sidoli, Simone; Wang, Leilei; Wang, Wenjun; Guo, Lin; Jensen, Ole Nørregaard; Zheng, Ling

    2014-01-01

    We present a detailed quantitative map of single and coexisting histone post-translational modifications (PTMs) in rat retinas affected by ischemia and reperfusion (I/R) injury. Retinal I/R injury contributes to serious ocular diseases, which can lead to vision loss and blindness. We applied linear...... provides a sensitive and accurate way to dissect the changes in the histone code after retinal injury. Specifically, DNA damage associated histone PTMs may contribute to neurovascular degeneration during the process of ischemia/reperfusion injury....

  5. Genomic analysis of post-mating changes in the honey bee queen (Apis mellifera

    Directory of Open Access Journals (Sweden)

    Richard Freddie-Jeanne

    2008-05-01

    Full Text Available Abstract Background The molecular mechanisms underlying the post-mating behavioral and physiological transitions undergone by females have not been explored in great detail. Honey bees represent an excellent model system in which to address these questions because they exhibit a range of "mating states," with two extremes (virgins and egg-laying, mated queens that differ dramatically in their behavior, pheromone profiles, and physiology. We used an incompletely-mated mating-state to understand the molecular processes that underlie the transition from a virgin to a mated, egg-laying queen. We used same-aged virgins, queens that mated once but did not initiate egg-laying, and queens that mated once and initiated egg-laying. Results Differences in the behavior and physiology among groups correlated with the underlying variance observed in the top 50 predictive genes in the brains and the ovaries. These changes were correlated with either a behaviorally-associated pattern or a physiologically-associated pattern. Overall, these results suggest that the brains and the ovaries of queens are uncoupled or follow different timescales; the initiation of mating triggers immediate changes in the ovaries, while changes in the brain may require additional stimuli or take a longer time to complete. Comparison of our results to previous studies of post-mating changes in Drosophila melanogaster identified common biological processes affected by mating, including stress response and alternative-splicing pathways. Comparison with microarray data sets related to worker behavior revealed no obvious correlation between genes regulated by mating and genes regulated by behavior/physiology in workers. Conclusion Studying the underlying molecular mechanisms of post-mating changes in honey bee queens will not only give us insight into how molecular mechanisms regulate physiological and behavioral changes, but they may also lead to important insights into the evolution of

  6. An Ex-Post Economic Analysis of the Hybrix5 Sweet Corn Breeding Program in Queensland

    OpenAIRE

    Franco-Dixon, Mary Ann

    2009-01-01

    The $2.1 million invested for the sweet corn research breeding program resulted in the release of Hybrix5 in 1995 which is a new sweet corn variety with improved insect and disease resistance. Based on the ex-post evaluation of this research program, it was estimated that the net benefits of the program up to 2006 (in 2006 dollars) is around $3 million. The producer benefits are 4.5 times the costs of R&D. Extending the period up to 2012 (20 years) resulted in estimated net benefits of around...

  7. HIGHER EDUCATION MARKET IN CENTRAL AND EASTERN EUROPE IN THE POST-CRISIS PERIOD. COMPARATIVE ANALYSIS.

    OpenAIRE

    HALANGESCU CONSTANTIN

    2015-01-01

    With a target (failed, by the way) to become the most competitive and dynamic economy in the world, Europe has scaled the market of higher education. Adopting a questioning approach in research methodology, based on interrogations such as „How the economic crisis has affected the Higher Education market in Emerging Europe?”, „How much the European Union regulations in the field of student fees, ante, during and post-crisis time affecting the employment market and equitable access at fair educ...

  8. Hazard Analysis for Post-Fire Debris-Flow Potential in Arizona

    Science.gov (United States)

    Youberg, A.; Koestner, K. A.; Schiefer, E.; Neary, D. G.

    2011-12-01

    Several large, devastating wildfires occurred in Arizona during the past 2 years, after a 4-year period without any large wildfires. In June, 2010, the human-caused Schultz Fire near Flagstaff burned 6,100 ha of mostly steep terrain. Subsequent rains from the 4th wettest monsoon on record produced numerous debris flows, significant erosion, and substantial flooding of the downslope residential areas. In May and June of 2011, 3 very large human-caused wildfires (Wallow, Horseshoe 2, and Monument Fires) burned over 320,000 ha, posing serious threats to communities below burned slopes. The Burned Area Emergency Response (BAER) teams, in need of a rapid method to assess the potential for post-fire debris flows, turned to models developed by the USGS for this purpose [Cannon and others, 2010, GSA Bull, 122(1-2), 127-144]. These models, while providing quick results, have not been evaluated for use in Arizona's varied physiographic provinces. Here we use data from the Schultz Fire to compare basin responses with those predicted by the USGS post-fire debris-flow models. Data from the Schultz Fire includes detailed field documentation of debris-flow occurrence and runout distances, 1:12,000 stereo aerial photographs, high-resolution digital elevation models (DEMs) and tipping-bucket rainfall data. These data document debris-flow producing storms, basin response, and the extent of debris-flow runout, and provide estimates of debris-flow volumes. The hydrologic responses from 30 small, steep, upper basins burned by the Schultz Fire were assessed for debris or flood flow occurrences. Nineteen basins produced debris flows during a July 20th storm that had a peak 10-minute intensity of 24 mm. A second storm on August 16th, with a peak 10-minute intensity of 15 mm, produced additional debris flows in several of the same basins. Of the 30 basins assessed, 19 were completely burned; four at high severity and 12 at moderate to high severity. The basin with the smallest burned area

  9. Glycoproteomic analysis of seven major allergenic proteins reveals novel post-translational modifications

    DEFF Research Database (Denmark)

    Halim, Adnan; Carlsson, Michael C; Mathiesen, Caroline Benedicte K;

    2015-01-01

    Allergenic proteins such as grass pollen and house dust mite (HDM) proteins are known to trigger hypersensitivity reactions of the immune system, leading to what is commonly known as allergy. Key allergenic proteins including sequence variants have been identified but characterization of their post......-translational modifications (PTMs) is still limited. Here, we present a detailed PTM(1) characterization of a series of the main and clinically relevant allergens used in allergy tests and vaccines. We employ Orbitrap-based mass spectrometry with complementary fragmentation techniques (HCD/ETD) for site-specific PTM...

  10. Meaningful-Experience Creation and Event Management A Post-Event Analysis of Copenhagen Carnival 2009

    OpenAIRE

    Sarah Holst Kjær

    2011-01-01

    A carnival is a cultural event within the experience economy, and can be considered an activity of added value to a city when creating place-awareness for tourists and residents. ‘Culture’ is used as a way to regenerate post-industrial and run down places, when studying EU – as well as Nordic – cultural policy reports. This might be too much to expect from the cultural sector though. Amongst other external factors, cultural policy ideals co-create and affect the experiential content of an eve...

  11. Post-translational modifications in Pseudomonas aeruginosa revolutionized by proteomic analysis.

    Science.gov (United States)

    Ouidir, Tassadit; Jouenne, Thierry; Hardouin, Julie

    2016-06-01

    Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections in vulnerable individuals. It is known that post-translational modifications (PTMs) play a key role in bacterial physiology. Their characterization is still challenging and the recent advances in proteomics allow large-scale and high-throughput analyses of PTMs. Here, we provide an overview of proteomic data about the modified proteins in P. aeruginosa. We emphasize the significant contribution of proteomics in knowledge enhancement of PTMs (phosphorylation, N-acetylation and glycosylation) and we discuss their importance in P. aeruginosa physiology. PMID:26952777

  12. Correlation between homocysteine and Vitamin B12 levels: A post-hoc analysis from North-West India

    Directory of Open Access Journals (Sweden)

    Sunil Kumar Raina

    2015-01-01

    Full Text Available Background: Homocysteine has been shown to be a risk factor for cardiovascular disease and is a degradation product of sulfur containing amino acids. The aim of this post-hoc analysis was aimed at arriving at homocysteine levels among voluntarily consenting healthy adults in the context of other hematological parameters. Methods: The data for this post-hoc analysis were derived from an observational study carried out at a medical college in rural North-west India. Results: About 77.42% of those participants enrolled in this study having serum homocysteine level more than 30 μmol/L were seen to possess suboptimal serum Vitamin B 12 (<200 pg/ml. On subjecting data to regression analysis, serum homocysteine was observed to possess an inverse correlation with serum level of Vitamin B 12, in general. Conclusions: Hyperhomocysteinemia observed in our study was sufficiently common and wholly ascribable to low Vitamin B 12 concentration as we did not find any case of subnormal serum folic acid level.

  13. A detailed analysis of messages posted on the ‘Turk-Orthopod’ mailing list over a 13-year period

    OpenAIRE

    Kose, Ozkan; Ozyurek, Selahattin; Duygun, Fatih; Guler, Ferhat; Turan, Adil; Arazi, Mehmet

    2015-01-01

    Objective: The objective of this study was to analyze the content of the ‘Turk-Orthopod’ mailing list between 2000 and 2013.Methods: All messages posted to the ‘Turk-Orthopod’ mailing list were documented and categorized by 4 orthopedic surgeons who were active members of the mailing list into 11 categories based on the content of the messages.Results: A total of 15,699 messages were evaluated and included in the analysis. The mean number of daily messages in 2000 was 0.98 messages/day and in...

  14. Comparative Proteome Analysis of Porcine Longissimus dorsi on the Basis of pH24 of Post-mortem Muscle

    OpenAIRE

    Juhyun Nam; Dong-Gi Lee; Joseph Kwon; Chi-Won Choi; Seong Hwa Park; Sang-Oh Kwon; Jong Hyun Jung; Hwa Choon Park; Beom Young Park; Ik Soon Jang; Woo Young Bang; Chul Wook Kim; Jong-Soon Choi

    2012-01-01

    To identify proteins contributing to meat quality, a comparative shotgun proteomic profiling of Berkshire longissimus dorsi muscles was conducted in high pH group (HpHG) and low pH group (LpHG) based on 24 hour post-mortem pH. Triplicate liquid chromatography–tandem mass spectrometry analysis identified a total of 208 and 204 proteins in the HpHG and LpHG, respectively. A total of 128 proteins were classified on the basis of molecular function, cellular components, and biological process by g...

  15. Post test analysis of counterpart tests in LOBI, SPES, BETHSY, LSTF facilities performed with the CATHARE2 code

    International Nuclear Information System (INIS)

    The present paper deals with the evaluation of results from the application of the thermalhydraulic system code CATHARE2V1.3U to the post test analysis of six co-called counterpart tests performed in four PWR facilities. These are LOBI/Mod2, SPES, BETHSY and LSTF facilities operated respectively at the European Research Centre of Ispra (Italy), at Piacenza (Italy), at Grenoble (France) and at Tokaj Mura (Japan). The activity is a part of a wide research having as main objective the evaluation of scaling capabilities of the CATHARE2 code. (author)

  16. Analysis of the Relationship between Antioxidant Enzyme Gene Polymorphisms and Their Activity in Post-Traumatic Gonarthrosis.

    Science.gov (United States)

    Vnukov, V V; Panina, S B; Milyutina, N P; Krolevets, I V; Zabrodin, M A

    2016-05-01

    Analysis of polymorphisms of genes encoding antioxidant enzymes SOD1 (G7958A), SOD2 (T58C), CAT (C-262T), and GSTP1 (Ile105Val) in 93 patients with post-traumatic gonarthrosis showed that GSTP1 Ile105Val polymorphism is often associated with heterozygous mutation in catalase gene CAT C-262T. In gonarthrosis, catalase activity in peripheral blood mononuclear cells in patients with CT genotype of the C-262T locus of CAT gene more than 2-fold surpassed that in CC genotype and more than 50% surpassed the normal. Changes in the balance of activity of antioxidant enzymes can affect viability of mononuclear cells. PMID:27270931

  17. Borderline pulmonary arterial pressure in systemic sclerosis patients: a post-hoc analysis of the DETECT study

    OpenAIRE

    Visovatti, Scott H; Distler, Oliver; Coghlan, J.; Denton, Christopher P; Grünig, Ekkehard; Bonderman, Diana; Müller-Ladner, Ulf; Pope, Janet E; Vonk, Madelon C; Seibold, James R; Torres-Martin, Juan-Vicente; Doelberg, Martin; Chadha-Boreham, Harbajan; Rosenberg, Daniel M; McLaughlin, Vallerie V.

    2014-01-01

    Introduction Patients with mean pulmonary artery pressures (mPAP) of 21 to 24 mm Hg have a so-called borderline elevation of mPAP (BoPAP)—a condition thought to represent early-stage pulmonary arterial vasculopathy. Based on the DETECT study, this post-hoc analysis examined patient characteristics of systemic sclerosis (SSc) patients with normal mPAP, BoPAP and elevated mPAP, fulfilling pulmonary arterial hypertension (PAH) criteria. Methods Adult patients with a duration of SSc more than 3 y...

  18. Effective strategies for weight loss in post-partum women: a systematic review and meta-analysis.

    Science.gov (United States)

    Lim, S; O'Reilly, S; Behrens, H; Skinner, T; Ellis, I; Dunbar, J A

    2015-11-01

    Post-partum weight loss is critical to preventing and managing obesity in women, but the results from lifestyle interventions are variable and the components associated with successful outcomes are not yet clearly identified. This study aimed to identify lifestyle intervention strategies associated with weight loss in post-partum women. MEDLINE, EMBASE, PubMed, CINAHL and four other databases were searched for lifestyle intervention studies (diet or exercise or both) in post-partum women (within 12 months of delivery) published up to July 2014. The primary outcome was weight loss. Subgroup analyses were conducted for self-monitoring, individual or group setting, intervention duration, intervention types, the use of technology as a support, and home- or centre-based interventions. From 12,673 studies, 46 studies were included in systematic review and 32 randomized controlled trials were eligible for meta-analysis (1,892 women, age 24-36 years). Studies with self-monitoring had significantly greater weight lost than those without (-4.61 kg [-7.08, -2.15] vs. -1.34 kg [-1.66, -1.02], P = 0.01 for subgroup differences). Diet and physical activity when combined were significantly more effective on weight loss compared with physical activity alone (-3.24 kg [-4.59, -1.90] vs. -1.63 kg [-2.16, -1.10], P < 0.001 for subgroup differences). Lifestyle interventions that use self-monitoring and take a combined diet-and-exercise approach have significantly greater weight loss in post-partum women. PMID:26313354

  19. Regulation of expression of two LY-6 family genes by intron retention and transcription induced chimerism

    Directory of Open Access Journals (Sweden)

    Mallya Meera

    2008-09-01

    Full Text Available Abstract Background Regulation of the expression of particular genes can rely on mechanisms that are different from classical transcriptional and translational control. The LY6G5B and LY6G6D genes encode LY-6 domain proteins, whose expression seems to be regulated in an original fashion, consisting of an intron retention event which generates, through an early premature stop codon, a non-coding transcript, preventing expression in most cell lines and tissues. Results The MHC LY-6 non-coding transcripts have shown to be stable and very abundant in the cell, and not subject to Nonsense Mediated Decay (NMD. This retention event appears not to be solely dependent on intron features, because in the case of LY6G5B, when the intron is inserted in the artificial context of a luciferase expression plasmid, it is fully spliced but strongly stabilises the resulting luciferase transcript. In addition, by quantitative PCR we found that the retained and spliced forms are differentially expressed in tissues indicating an active regulation of the non-coding transcript. EST database analysis revealed that these genes have an alternative expression pathway with the formation of Transcription Induced Chimeras (TIC. This data was confirmed by RT-PCR, revealing the presence of different transcripts that would encode the chimeric proteins CSNKβ-LY6G5B and G6F-LY6G6D, in which the LY-6 domain would join to a kinase domain and an Ig-like domain, respectively. Conclusion In conclusion, the LY6G5B and LY6G6D intron-retained transcripts are not subjected to NMD and are more abundant than the properly spliced forms. In addition, these genes form chimeric transcripts with their neighbouring same orientation 5' genes. Of interest is the fact that the 5' genes (CSNKβ or G6F undergo differential splicing only in the context of the chimera (CSNKβ-LY6G5B or G6F-LY6G6C and not on their own.

  20. Quantitative Analysis of Defects at the Dentin-Post Space in Endodontically Treated Teeth

    Directory of Open Access Journals (Sweden)

    Mariasevera Di Comite

    2015-06-01

    Full Text Available The objective of this study was to assess frequency and extension of the defects affecting the dentin-post interface after using different combinations of irrigants and sealers. The experimental work was conducted on single-rooted teeth extracted for orthodontic reasons. The specimens were divided into different groups, according to irrigant and endodontic cement utilized, and endodontically instrumented. After fiberglass posts cementation, cross sections were obtained at apical, middle and coronal level of the root and submitted to quantitative analyses. Different types of defects were found: bubbles, bonding defects, polymerization defect, and cement residues. The percent extension of each defect and its frequency were related to the specific irrigant/sealer combination and to the root level. Detachments of the material from dentin were found only at apical and middle levels. Chlorhexidine digluconate seems to have more beneficial effects if compared to sodium hypochlorite: samples prepared with chlorhexidine digluconate showed a higher performance, with roots including null to few defects. In detail, samples treated with chlorhexidine digluconate and Pulp Canal Sealer showed the lowest frequency and the smallest dimension of defects.

  1. Semen analysis in post-pubertal patients with posterior urethral valves: a pilot study.

    Science.gov (United States)

    Puri, A; Gaur, K K; Kumar, A; Bhatnagar, V

    2002-03-01

    The issues of sexual function and fertility are becoming relevant in patients with posterior urethral valves (PUV), as more of them reach adulthood. To evaluate the semen of post-pubertal patients with PUV as a determinant of future fertility, all such patients (age >16 years) attending the follow-up urology clinic of our department from 1985 to December 1999 were contacted. Of the nine patients contacted, eight agreed to form the study group. All eight patients were asked to provide a post-masturbation semen sample and urine. Semen was analysed for pH, viscosity, liquefaction time, sperm morphology, sperm count, motility, and agglutination. The patients ages ranged from 16 to 21 years (mean 17.5 years). One patient with chronic renal failure awaiting a renal transplant refused to give a semen sample; two tried but failed to ejaculate on three consecutive visits. Their urine was negative for sperm. Of the five patients who gave semen, the liquefaction time was high in two. pH ranged from 7.2 to 8, sperm counts were 24-80 million. None of the patients had oligospermia. Abnormal sperm agglutination was present in four cases; a higher percentage of immotile sperm was also present in four. Semen abnormalities in the form of increased liquefaction time, abnormal sperm agglutination, and a high percentage of immotile sperm were thus seen in the present study. The bearing of these findings on subsequent sexual function and fertility remains a matter of speculation. PMID:11956780

  2. Antigenic properties of a transport-competent influenza HA/HIV Env chimeric protein

    International Nuclear Information System (INIS)

    The transmembrane subunit (gp41) of the HIV Env glycoprotein contains conserved neutralizing epitopes which are not well-exposed in wild-type HIV Env proteins. To enhance the exposure of these epitopes, a chimeric protein, HA/gp41, in which the gp41 of HIV-1 89.6 envelope protein was fused to the C-terminus of the HA1 subunit of the influenza HA protein, was constructed. Characterization of protein expression showed that the HA/gp41 chimeric proteins were expressed on cell surfaces and formed trimeric oligomers, as found in the HIV Env as well as influenza HA proteins. In addition, the HA/gp41 chimeric protein expressed on the cell surface can also be cleaved into 2 subunits by trypsin treatment, similar to the influenza HA. Moreover, the HA/gp41 chimeric protein was found to maintain a pre-fusion conformation. Interestingly, the HA/gp41 chimeric proteins on cell surfaces exhibited increased reactivity to monoclonal antibodies against the HIV Env gp41 subunit compared with the HIV-1 envelope protein, including the two broadly neutralizing monoclonal antibodies 2F5 and 4E10. Immunization of mice with a DNA vaccine expressing the HA/gp41 chimeric protein induced antibodies against the HIV gp41 protein and these antibodies exhibit neutralizing activity against infection by an HIV SF162 pseudovirus. These results demonstrate that the construction of such chimeric proteins can provide enhanced exposure of conserved epitopes in the HIV Env gp41 and may represent a novel vaccine design strategy for inducing broadly neutralizing antibodies against HIV

  3. Ex-post analysis of forecasting and regulations of italian national energy plan of 1988; Analisi ex-post delle previsioni e delle direttive del Piano Energetico Nazionale del 1988

    Energy Technology Data Exchange (ETDEWEB)

    Albergamo, V. [ENEA, Rome (Italy)

    1998-07-01

    This paper analyze the forecasting and recommendations of national energy plan of 1988 is carried out an ex-post analysis. [Italian] Il principale obiettivo del presente studio e' quello di valutare il ruolo svolto dal Piano Energetico Nazionale del 1988 nell'evoluzione del sistema energetico nazionale.

  4. Effectiveness of bee venom acupuncture in alleviating post-stroke shoulder pain:a systematic review and meta-analysis

    Institute of Scientific and Technical Information of China (English)

    Sung Min Lim; Sook-Hyun Lee

    2015-01-01

    BACKGROUND:Shoulder pain is a common complication of stroke. Bee venom acupuncture (BVA) is increasingly used in the treatment of post-stroke shoulder pain. OBJECTIVE: To summarize and evaluate evidence on the effectiveness of BVA in relieving shoulder pain after stroke. SEARCH STRATEGY: Nine databases, namely MEDLINE, EMBASE, the Cochrane Library, the China National Knowledge Infrastructure (CNKI), the Japan Science and Technology Information Aggregator, Electronic (J-STAGE), and four Korean medical databases, namely, the National Assembly Library, the Research Information Service System, the National Discovery for Science Leaders, and OASIS, were searched from their inception through August 2014 without language restrictions. INCLUSION CRITERIA: Randomized controled trials (RCTs) were included if BVA was used at acupoints as the sole treatment, or as an adjunct to other treatments, for shoulder pain after stroke. DATA EXTRACTION AND ANALYSIS:Two review authors independently selected trials for inclusion, assessed methodological quality and extracted data. RESULTS: A total of 138 potentialy relevant articles were identiifed, 4 of which were RCTs that met our inclusion criteria. The quality of studies included was generaly low, and a preponderance of positive results was demonstrated. Al four trials reported favorable effects of BVA on shoulder pain after stroke. Two RCTs assessing the effects of BVA on post-stroke shoulder pain, as opposed to saline injections, were included in the meta-analysis. Pain was signiifcantly lower for BVA than for saline injections (standardized mean difference on 10-cm visual analog scale: 1.46 cm, 95% CI = 0.30–2.62,P = 0.02, n = 86) CONCLUSION: This review provided evidence suggesting that BVA is effective in relieving shoulder pain after stroke. However, further studies are needed to conifrm the role of BVA in aleviating post-stroke shoulder pain. Future studies should be conducted with large samples and rigorous study designs.

  5. Analysis of the thoracic aorta using a semi-automated post processing tool

    Energy Technology Data Exchange (ETDEWEB)

    Entezari, Pegah, E-mail: p-entezari@northwestern.edu [Department of Radiology, Cardiovascular Imaging, Northwestern University, Chicago, IL (United States); Kino, Aya, E-mail: ayakino@gmail.com [Department of Radiology, Cardiovascular Imaging, Northwestern University, Chicago, IL (United States); Honarmand, Amir R., E-mail: arhonarmand@yahoo.com [Department of Radiology, Cardiovascular Imaging, Northwestern University, Chicago, IL (United States); Galizia, Mauricio S., E-mail: maugalizia@yahoo.com.br [Department of Radiology, Cardiovascular Imaging, Northwestern University, Chicago, IL (United States); Yang, Yan, E-mail: yyang@vitalimages.com [Vital images Inc, Minnetonka, MN (United States); Collins, Jeremy, E-mail: collins@fsm.northwestern.edu [Department of Radiology, Cardiovascular Imaging, Northwestern University, Chicago, IL (United States); Yaghmai, Vahid, E-mail: vyaghmai@northwestern.edu [Department of Radiology, Cardiovascular Imaging, Northwestern University, Chicago, IL (United States); Carr, James C., E-mail: jcarr@northwestern.edu [Department of Radiology, Cardiovascular Imaging, Northwestern University, Chicago, IL (United States)

    2013-09-15

    Objective: To evaluates a semi-automated method for Thoracic Aortic Aneurysm (TAA) measurement using ECG-gated Dual Source CT Angiogram (DSCTA). Methods: This retrospective HIPAA compliant study was approved by our IRB. Transaxial maximum diameters of outer wall to outer wall were studied in fifty patients at seven anatomic locations of the thoracic aorta: annulus, sinus, sinotubular junction (STJ), mid ascending aorta (MAA) at the level of right pulmonary artery, proximal aortic arch (PROX) immediately proximal to innominate artery, distal aortic arch (DIST) immediately distal to left subclavian artery, and descending aorta (DESC) at the level of diaphragm. Measurements were performed using a manual method and semi-automated software. All readers repeated their measurements. Inter-method, intra-observer and inter-observer agreements were evaluated according to intraclass correlation coefficient (ICC) and Bland–Altman plot. The number of cases with manual contouring or center line adjustment for the semi-automated method and also the post-processing time for each method were recorded. Results: The mean difference between semi-automated and manual methods was less than 1.3 mm at all seven points. Strong inter-method, inter-observer and intra-observer agreement was recorded at all levels (ICC ≥ 0.9). The maximum rate of manual adjustment of center line and contour was at the level of annulus. The average time for manual post-processing of the aorta was 19 ± 0.3 min, while it took 8.26 ± 2.1 min to do the measurements with the semi-automated tool (Vitrea version 6.0.0.1 software). The center line was edited manually at all levels, with most corrections at the level of annulus (60%), while the contour was adjusted at all levels with highest and lowest number of corrections at the levels of annulus and DESC (75% and 0.07% of the cases), respectively. Conclusion: Compared to the commonly used manual method, semi-automated measurement of vessel dimensions is

  6. Site-specific climate analysis elucidates revegetation challenges for post-mining landscapes in eastern Australia

    Science.gov (United States)

    Audet, P.; Arnold, S.; Lechner, A. M.; Baumgartl, T.

    2013-10-01

    In eastern Australia, the availability of water is critical for the successful rehabilitation of post-mining landscapes and climatic characteristics of this diverse geographical region are closely defined by factors such as erratic rainfall and periods of drought and flooding. Despite this, specific metrics of climate patterning are seldom incorporated into the initial design of current post-mining land rehabilitation strategies. Our study proposes that a few common rainfall parameters can be combined and rated using arbitrary rainfall thresholds to characterise bioregional climate sensitivity relevant to the rehabilitation these landscapes. This approach included assessments of annual rainfall depth, average recurrence interval of prolonged low intensity rainfall, average recurrence intervals of short or prolonged high intensity events, median period without rain (or water-deficit) and standard deviation for this period in order to address climatic factors such as total water availability, seasonality and intensity - which were selected as potential proxies of both short- and long-term biological sensitivity to climate within the context of post-disturbance ecological development and recovery. Following our survey of available climate data, we derived site "climate sensitivity" indexes and compared the performance of 9 ongoing mine sites: Weipa, Mt. Isa and Cloncurry, Eromanga, Kidston, the Bowen Basin (Curragh), Tarong, North Stradbroke Island, and the Newnes Plateau. The sites were then ranked from most-to-least sensitive and compared with natural bioregional patterns of vegetation density using mean NDVI. It was determined that regular rainfall and relatively short periods of water-deficit were key characteristics of sites having less sensitivity to climate - as found among the relatively more temperate inland mining locations. Whereas, high rainfall variability, frequently occurring high intensity events, and (or) prolonged seasonal drought were primary

  7. Site-specific climate analysis elucidates revegetation challenges for post-mining landscapes in eastern Australia

    Directory of Open Access Journals (Sweden)

    P. Audet

    2013-10-01

    Full Text Available In eastern Australia, the availability of water is critical for the successful rehabilitation of post-mining landscapes and climatic characteristics of this diverse geographical region are closely defined by factors such as erratic rainfall and periods of drought and flooding. Despite this, specific metrics of climate patterning are seldom incorporated into the initial design of current post-mining land rehabilitation strategies. Our study proposes that a few common rainfall parameters can be combined and rated using arbitrary rainfall thresholds to characterise bioregional climate sensitivity relevant to the rehabilitation these landscapes. This approach included assessments of annual rainfall depth, average recurrence interval of prolonged low intensity rainfall, average recurrence intervals of short or prolonged high intensity events, median period without rain (or water-deficit and standard deviation for this period in order to address climatic factors such as total water availability, seasonality and intensity – which were selected as potential proxies of both short- and long-term biological sensitivity to climate within the context of post-disturbance ecological development and recovery. Following our survey of available climate data, we derived site "climate sensitivity" indexes and compared the performance of 9 ongoing mine sites: Weipa, Mt. Isa and Cloncurry, Eromanga, Kidston, the Bowen Basin (Curragh, Tarong, North Stradbroke Island, and the Newnes Plateau. The sites were then ranked from most-to-least sensitive and compared with natural bioregional patterns of vegetation density using mean NDVI. It was determined that regular rainfall and relatively short periods of water-deficit were key characteristics of sites having less sensitivity to climate – as found among the relatively more temperate inland mining locations. Whereas, high rainfall variability, frequently occurring high intensity events, and (or prolonged seasonal

  8. Measurement and analysis of geometric parameters of human carotid bifurcation using image post-processing technique

    International Nuclear Information System (INIS)

    Objective: To investigate variation in the carotid bifurcation geometry of adults of different age by MR angiography images combining image post-processing technique. Methods: Images of the carotid bifurcations of 27 young adults (≤40 years old) and 30 older subjects ( > 40 years old) were acquired via contrast-enhanced MR angiography. Three dimensional (3D) geometries of the bifurcations were reconstructed and geometric parameters were measured by post-processing technique. Results: The geometric parameters of the young versus older groups were as follows: bifurcation angle (70.268 degree± 16.050 degree versus 58.857 degree±13.294 degree), ICA angle (36.893 degree±11.837 degree versus 30.275 degree±9.533 degree), ICA planarity (6.453 degree ± 5.009 degree versus 6.263 degree ±4.250 degree), CCA tortuosity (0.023±0.011 versus 0.014± 0.005), ICA tortuosity (0.070±0.042 versus 0.046±0.022), ICA/CCA diameter ratio (0.693± 0.132 versus 0.728±0.106), ECA/CCA diameter ratio (0.750±0.123 versus 0.809±0.122), ECA/ ICA diameter ratio (1.103±0.201 versus 1.127±0.195), bifurcation area ratio (1.057±0.281 versus 1.291±0.252). There was significant statistical difference between young group and older group in-bifurcation angle, ICA angle, CCA tortuosity, ICA tortuosity, ECA/CCA and bifurcation area ratio (F= 17.16, 11.74, 23.02, 13.38, 6.54, 22.80, respectively, P<0.05). Conclusions: MR angiography images combined with image post-processing technique can reconstruct 3D carotid bifurcation geometry and measure the geometric parameters of carotid bifurcation in vivo individually. It provides a new and convenient method to investigate the relationship of vascular geometry and flow condition with atherosclerotic pathological changes. (authors)

  9. Analysis of the thoracic aorta using a semi-automated post processing tool

    International Nuclear Information System (INIS)

    Objective: To evaluates a semi-automated method for Thoracic Aortic Aneurysm (TAA) measurement using ECG-gated Dual Source CT Angiogram (DSCTA). Methods: This retrospective HIPAA compliant study was approved by our IRB. Transaxial maximum diameters of outer wall to outer wall were studied in fifty patients at seven anatomic locations of the thoracic aorta: annulus, sinus, sinotubular junction (STJ), mid ascending aorta (MAA) at the level of right pulmonary artery, proximal aortic arch (PROX) immediately proximal to innominate artery, distal aortic arch (DIST) immediately distal to left subclavian artery, and descending aorta (DESC) at the level of diaphragm. Measurements were performed using a manual method and semi-automated software. All readers repeated their measurements. Inter-method, intra-observer and inter-observer agreements were evaluated according to intraclass correlation coefficient (ICC) and Bland–Altman plot. The number of cases with manual contouring or center line adjustment for the semi-automated method and also the post-processing time for each method were recorded. Results: The mean difference between semi-automated and manual methods was less than 1.3 mm at all seven points. Strong inter-method, inter-observer and intra-observer agreement was recorded at all levels (ICC ≥ 0.9). The maximum rate of manual adjustment of center line and contour was at the level of annulus. The average time for manual post-processing of the aorta was 19 ± 0.3 min, while it took 8.26 ± 2.1 min to do the measurements with the semi-automated tool (Vitrea version 6.0.0.1 software). The center line was edited manually at all levels, with most corrections at the level of annulus (60%), while the contour was adjusted at all levels with highest and lowest number of corrections at the levels of annulus and DESC (75% and 0.07% of the cases), respectively. Conclusion: Compared to the commonly used manual method, semi-automated measurement of vessel dimensions is

  10. Construction, Expression and Characterization of a Chimeric Protein Targeting Carcinoembryonic Antigen in Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    LI Yang; HUA Shu-cheng; MA Cheng-yuan; YU Zhen-xiang; XU Li-jun; LI Dan; SUN Li-li; LI Xiao; PENG Li-ping

    2011-01-01

    The carcinoembryonic antigen(CEA) is an oncofetal glycoprotein known as an important clinical tumor marker and is overexpressed in several types of tumors, including colorectal and lung carcinomas. We constructed a chimeric protein that exhibits both specific binding and immune stimulating activities, by fusing staphylococcal enterotoxin A(SEA) to the C-terminus of an anti-CEA single-chain disulfide-stabilized Fv(scdsFv) antibody (single-chain-C-terminus/SEA, SC-C/SEA). The SC-C/SEA protein was expressed in Escherichia coli(E. coli), refolded, and purified on an immobilized Ni2+ affinity chromatography column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Western blot analysis reveal that the target protein was expressed sufficiently. We used immunofluorescence assays to demonstrate that SC-C/SEA could bind specifically to human lung carcinoma cells(A549), but almost human uterine cervix cells(HeLa). We also used the L-lactate dehydrogenase(LDH) release assay to show that SC-C/SEA elicits a strong A549 tumor-specific cytotoxic T lymphocyte(CTL) response in vitro. The results suggest that SC-C/SEA shows specific activity against CEA-positive cells and has potential application in CEA-targeted cancer immunotherapy.

  11. Chimeric calcium/calmodulin-dependent protein kinase in tobacco: differential regulation by calmodulin isoforms

    Science.gov (United States)

    Liu, Z.; Xia, M.; Poovaiah, B. W.

    1998-01-01

    cDNA clones of chimeric Ca2+/calmodulin-dependent protein kinase (CCaMK) from tobacco (TCCaMK-1 and TCCaMK-2) were isolated and characterized. The polypeptides encoded by TCCaMK-1 and TCCaMK-2 have 15 different amino acid substitutions, yet they both contain a total of 517 amino acids. Northern analysis revealed that CCaMK is expressed in a stage-specific manner during anther development. Messenger RNA was detected when tobacco bud sizes were between 0.5 cm and 1.0 cm. The appearance of mRNA coincided with meiosis and became undetectable at later stages of anther development. The reverse polymerase chain reaction (RT-PCR) amplification assay using isoform-specific primers showed that both of the CCaMK mRNAs were expressed in anther with similar expression patterns. The CCaMK protein expressed in Escherichia coli showed Ca2+-dependent autophosphorylation and Ca2+/calmodulin-dependent substrate phosphorylation. Calmodulin isoforms (PCM1 and PCM6) had differential effects on the regulation of autophosphorylation and substrate phosphorylation of tobacco CCaMK, but not lily CCaMK. The evolutionary tree of plant serine/threonine protein kinases revealed that calmodulin-dependent kinases form one subgroup that is distinctly different from Ca2+-dependent protein kinases (CDPKs) and other serine/threonine kinases in plants.

  12. Diverse hematological malignancies including hodgkin-like lymphomas develop in chimeric MHC class II transgenic mice.

    Directory of Open Access Journals (Sweden)

    Silke H Raffegerst

    Full Text Available A chimeric HLA-DR4-H2-E (DR4 homozygous transgenic mouse line spontaneously develops diverse hematological malignancies with high frequency (70%. The majority of malignancies were distributed equally between T and B cell neoplasms and included lymphoblastic T cell lymphoma (LTCL, lymphoblastic B cell lymphoma (LBCL, diffuse large B cell lymphoma (DLBCL, the histiocyte/T cell rich variant of DLBCL (DLBCL-HA/T cell rich DLBCL, splenic marginal zone lymphoma (SMZL, follicular B cell lymphoma (FBL and plasmacytoma (PCT. Most of these neoplasms were highly similar to human diseases. Also, some non-lymphoid malignancies such as acute myeloid leukemia (AML and histiocytic sarcoma were found. Interestingly, composite lymphomas, including Hodgkin-like lymphomas, were also detected that had CD30(+ Hodgkin/Reed-Sternberg (H/RS-like cells, representing a tumor type not previously described in mice. Analysis of microdissected H/RS-like cells revealed their origin as germinal center B cells bearing somatic hypermutations and, in some instances, crippled mutations, as described for human Hodgkin lymphoma (HL. Transgene integration in an oncogene was excluded as an exclusive driving force of tumorigenesis and age-related lymphoma development suggests a multi-step process. Thus, this DR4 line is a useful model to investigate common molecular mechanisms that may contribute to important neoplastic diseases in man.

  13. Modification of chimeric (2S, 3S)-butanediol dehydrogenase based on structural information.

    Science.gov (United States)

    Shimegi, Tomohito; Mochizuki, Kaito; Oyama, Takuji; Ohtsuki, Takashi; Kusunoki, Masami; Ui, Sadaharu

    2014-01-01

    A chimeric (2S, 3S)-butanediol dehydrogenase (cLBDH) was engineered to have the strict (S)-configuration specificity of the (2S, 3S)-BDH (BsLBDH) derived from Brevibacterium saccharolyticum as well as the enzymatic stability of the (2R, 3S)-BDH (KpMBDH) from Klebsiella pneumonia by swapping the domains of two native BDHs. However, while cLBDH possesses the stability, it lacks the specificity. In order to assist in the design a BDH having strict substrate specificity, an X-ray structural analysis of a cLBDH crystal was conducted at 1.58 Å. The results obtained show some readily apparent differences around the active sites of cLBDH and BsLBDH. Based on this structural information, a novel (2S, 3S)-BDH having a preferred specificity was developed by introducing a V254L mutation into cLBDH. The influence of this mutation on the stability of cLBDH was not evaluated. Nevertheless, the technique described herein is an effective method for the production of a tailor-made BDH. PMID:25612804

  14. An Analysis of Education Expenditures in the Welfare State in the Post 1980 Period in Turkey

    Directory of Open Access Journals (Sweden)

    Düriye Toprak

    2016-06-01

    Full Text Available  The welfare state that had become stronger gradually in the post-2nd World War has been in the process of restructuring after the 1980 period as a result of globalisation and neo-liberal policies. Social welfare state has had responsibilities in different fields ranging from health to education and from social security to poverty reduction. In Turkey, the education expenditures may be accepted as social expenditures contributing to general social welfare of the society. The goal of this study is to explain the social welfare state briefly, to review the methodological approaches to social expenditures in the context of the social welfare state, and to examine and compare the level of education expenditures in Turkey and some other countries. 

  15. Post-flight Analysis of Mars Science Laboratory Entry Aerothermal Environment and Thermal Protection System Response

    Science.gov (United States)

    White, Todd Richard; Mahazari, Milad; Bose, Deepak; Santos, Jose Antonio

    2013-01-01

    The Mars Science Laboratory successfully landed on the Martian surface on August 5th, 2012. The rover was protected from the extreme heating environments of atmospheric entry by an ablative heatshield. This Phenolic Impregnated Carbon Ablator heatshield was instrumented with a suite of embedded thermocouples, isotherm sensors, and pressure transducers. The sensors monitored the in-depth ablator response, as well as the surface pressure at discrete locations throughout the hypersonic deceleration. This paper presents a comparison of the flight data with post-entry estimates. An assessment of the aerothermal environments, as well as the in-depth response of the heatshield material is made, and conclusions regarding the overall performance of the ablator at the suite locations are presented.

  16. Alpha1-acid glycoprotein post-translational modifications: a comparative two dimensional electrophoresis based analysis

    Directory of Open Access Journals (Sweden)

    P. Roncada

    2010-04-01

    Full Text Available Alpha1-acid glycoprotein (AGP is an immunomodulatory protein expressed by hepatocytes in response to the systemic reaction that follows tissue damage caused by inflammation, infection or trauma. A proteomic approach based on two dimensional electrophoresis, immunoblotting and staining of 2DE gels with dyes specific for post-translational modifications (PTMs such as glycosylation and phosphorylation has been used to evaluate the differential interspecific protein expression of AGP purified from human, bovine and ovine sera. By means of these techniques, several isoforms have been identified in the investigated species: they have been found to change both with regard to the number of isoforms expressed under physiological condition and with regard to the quality of PTMs (i.e. different oligosaccharidic chains, presence/absence of phosphorilations. In particular, it is suggested that bovine serum AGP may have one of the most complex pattern of PTMs among serum proteins of mammals studied so far.

  17. GOTHIC analysis of post-accident hydrogen mixing behaviour in CANDU fuelling machine vault

    International Nuclear Information System (INIS)

    The GOTHIC code was used to assess the post-accident hydrogen gas mixing patterns in a CANDU reactor containment and demonstrate the acceptability of Ontario Hydro Nuclear's hydrogen mitigation methods. The fuelling machine vault, being a small volume room containing major reactor piping, is the room of most concern with respect to hydrogen concentrations. Detailed three dimensional modelling of the gas mixing patterns in the fuelling machine vault was completed. Results showed that, even without forced air circulation, there is enough dispersion of hydrogen to other parts of containment to preclude the build-up of sensitive mixtures in the vault. For a brief time during the peak period of hydrogen release, hydrogen concentrations rise to close to the lower ignition limit in a small portion of the vault, but these hydrogen-steam-air mixtures are considered acceptable. Natural mixing alone is sufficient to preclude damaging hydrogen burns. (author)

  18. Analysis of post-blasting source mechanisms of mining-induced seismic events in Rudna copper mine, Poland.

    Science.gov (United States)

    Caputa, Alicja; Rudzinski, Lukasz; Talaga, Adam

    2016-04-01

    Copper ore exploitation in the Lower Silesian Copper District, Poland (LSCD), is connected with many specific hazards. The most hazardous one is induced seismicity and rockbursts which follow strong mining seismic events. One of the most effective method to reduce seismic activity is blasting in potentially hazardous mining panels. This way, small to moderate tremors are provoked and stress accumulation is substantially reduced. This work presents an analysis of post-blasting events using Full Moment Tensor (MT) inversion at the Rudna mine, Poland using signals dataset recorded on underground seismic network. We show that focal mechanisms for events that occurred after blasts exhibit common features in the MT solution. The strong isotropic and small Double Couple (DC) component of the MT, indicate that these events were provoked by detonations. On the other hand, post-blasting MT is considerably different than the MT obtained for common strong mining events. We believe that seismological analysis of provoked and unprovoked events can be a very useful tool in confirming the effectiveness of blasting in seismic hazard reduction in mining areas.

  19. Analysis of post-blasting source mechanisms of mining-induced seismic events in Rudna copper mine, Poland

    Directory of Open Access Journals (Sweden)

    Caputa Alicja

    2015-10-01

    Full Text Available The exploitation of georesources by underground mining can be responsible for seismic activity in areas considered aseismic. Since strong seismic events are connected with rockburst hazard, it is a continuous requirement to reduce seismic risk. One of the most effective methods to do so is blasting in potentially hazardous mining panels. In this way, small to moderate tremors are provoked and stress accumulation is substantially reduced. In this paper we present an analysis of post-blasting events using Full Moment Tensor (MT inversion at the Rudna mine, Poland, underground seismic network. In addition, we describe the problems we faced when analyzing seismic signals. Our studies show that focal mechanisms for events that occurred after blasts exhibit common features in the MT solution. The strong isotropic and small Double Couple (DC component of the MT, indicate that these events were provoked by detonations. On the other hand, post-blasting MT is considerably different than the MT obtained for strong mining events. We believe that seismological analysis of provoked and unprovoked events can be a very useful tool in confirming the effectiveness of blasting in seismic hazard reduction in mining areas.

  20. Mass-spectrometry analysis of histone post-translational modifications in pathology tissue using the PAT-H-MS approach.

    Science.gov (United States)

    Noberini, Roberta; Pruneri, Giancarlo; Minucci, Saverio; Bonaldi, Tiziana

    2016-06-01

    Aberrant histone post-translational modifications (hPTMs) have been implicated with various pathologies, including cancer, and may represent useful epigenetic biomarkers. The data described here provide a mass spectrometry-based quantitative analysis of hPTMs from formalin-fixed paraffin-embedded (FFPE) tissues, from which histones were extracted through the recently developed PAT-H-MS method. First, we analyzed FFPE samples from mouse spleen and liver or human breast cancer up to six years old, together with their corresponding fresh frozen tissue. We then combined the PAT-H-MS approach with a histone-focused version of the super-SILAC strategy-using a mix of histones from four breast cancer cell lines as a spike-in standard- to accurately quantify hPTMs from breast cancer specimens belonging to different subtypes. The data, which are associated with a recent publication (Pathology tissue-quantitative mass spectrometry analysis to profile histone post-translational modification patterns in patient samples (Noberini, 2015) [1]), are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD002669. PMID:27408908

  1. Analysis on Biomechanical Characteristics of Post-operational Vertebral C5-C6 Segments

    Directory of Open Access Journals (Sweden)

    Heqiang Tian

    2016-03-01

    Full Text Available Both anterior cervical decompression and fusion (ACDF and artificial cervical disc replacement (ACDR have obvious advantages in the treatment of cervical spondylosis. To analyze the operation results, it is absolutely necessary to study the biomechanics of the movement range of post-operational vertebral C5-C6 segments, especially the biomechanical characteristics in cervical tissues in actual movements. In this study, using the human vertebral 3D graph gained by imaging diagnosis (CT, a vertebral solid model is established by the 3D reconstruction algorithm and reverse engineering technology. After that, with cervical soft tissue structure added to the solid model and set with a joint contact mechanism, a finite element model with a complete, accurate cervical C5-C6 kinematic unit is constructed, based on relevant physiological anatomical knowledge. This model includes vertebral segments, an intervertebral disc, ligament and zygopophysis in the cervical C5-C6 kinematic unit. In the created vertebral finite element model, the model is amended, referring to ACDF and ACDR, and the load and constraint are applied to a normal group, a fusion group and a displacement group, so as to analyze the biomechanical characteristics of the cervical vertebra after ACDF and ACDR. By comparing the finite element simulation results of different surgeries, this paper is intended to evaluate the functions and biomechanical behaviors of the post-operational vertebra, and explore the influence of the operation on the biomechanical stability of the cervical vertebra. This will provide theoretical guidance for implementation and optimization of ACDF and ACDR.

  2. Heart rate variability analysis using a ballistocardiogram during Valsalva manoeuvre and post exercise

    International Nuclear Information System (INIS)

    We introduced a novel non-constrained technique for estimating heart rate variability (HRV) using a ballistocardiogram (BCG). To assess whether the BCG signal can be used to analyse the cardiac autonomic modulation, HRV parameters derived from the BCG signal (ballistocardiographic HRV, B-HRV) were statistically compared with the HRV parameters from the ECG signal during rest and under two different experimental conditions that induce cardiac autonomic rhythm changes: the Valsalva manoeuvre and static exercise. Time domain, frequency domain and nonlinear analyses were individually performed on 15 healthy subjects to assess whether the BCG can be used to analyse the cardiac autonomic modulation under each condition. For all subjects, the proposed method had averages of relative errors of 5.01 ± 4.72, 5.64 ± 4.83 and 5.98 ± 5.80% for resting, Valsalva and post-exercise sessions, respectively, and the correlation coefficients between the reference (ECG) and proposed (BCG) methods are 0.97, 0.98 and 0.98, for resting, Valsalva and post-exercise sessions, respectively. During cardiac autonomic changes, the B-HRV parameters changed in a pattern that is very similar to the variations in the HRV parameters based on Student's t-test results. In addition, some of the B-HRV parameters changed according to cardiac autonomic rhythms controlled by sympathetic and parasympathetic activities during the experiments. These findings indicate that BCG can provide an accurate and reliable means to evaluate autonomic system activation by HRV in its unconstrained way

  3. Post-processing of Engineering Analysis Results for Visualization in VR Systems

    OpenAIRE

    Maleshkov, Stoyan; Chotrov, Dimo

    2013-01-01

    The applicability of Virtual Reality for evaluating engineering analysis results is beginning to receive increased appreciation in the last years. The problem many engineers are still facing is how to import their model together with the analysis results in a virtual reality environment for exploration and results validation. In this paper we propose an algorithm for transforming model data and results from finite element analysis (FEA) solving application to a format easily interpretable by ...

  4. Immunomodulatory Effects of Different Cellular Therapies of Bone Marrow Origin on Chimerism Induction and Maintenance Across MHC Barriers in a Face Allotransplantation Model.

    Science.gov (United States)

    Hivelin, Mikael; Klimczak, Aleksandra; Cwykiel, Joanna; Sonmez, Erhan; Nasir, Serdar; Gatherwright, James; Siemionow, Maria

    2016-08-01

    Many more patients would benefit from vascularized composite allotransplantation if less toxic and safer immunosuppressive protocols will become available. Tolerance induction protocols with donor cells co-transplantation are one of the promising pathways to reduce maintenance immunosupressive regimens. We investigated the role of donor bone marrow cells (BMC), mesenchymal stromal cells (MSC) and in vivo created chimeric cells (CC) used as supportive therapies in a fully MHC-mismatched rat face transplantation model. Twenty-four fully MHC-mismatched hemiface transplantations were performed between ACI (RT1(a)) donors and Lewis (RT1(l)) recipients under combined seven-day immunosuppressive regimen of anti-αβ-T-cell receptor (TCR) monoclonal antibody and cyclosporin A. We studied four experimental groups-group 1: no cellular therapy; group 2: supportive therapy with BMC; group 3: supportive therapy with MSC; group 4: supportive therapy with CC generated in a primary chimera. We evaluated clinical and histological rejection grades, transplanted cells migration, donor-specific chimerism in the peripheral blood and bone marrow compartments, and CD4(+)/CD25(+) T-cell levels. Face allograft rejection was observed at 26.8 ± 0.6 days post-transplant (PT) in the absence of cellular therapy, at 34.5 ± 1.1 days for group 2, 29.3 ± 0.8 days for group 3, and 30.3 ± 1.38 PT for group 4. The longest survival was observed in allografts supported by co-transplantation of BMC. All support in cellular therapies delayed face allograft rejection by chimerism induction and/or immunomodulatory properties of co-transplanted cells. Survival time was comparable between groups, however, further studies, with different cell dosages, delivery routes and delivery times are required. PMID:26708158

  5. Younger women's experiences of deciding against delayed breast reconstruction post-mastectomy following breast cancer: An interpretative phenomenological analysis.

    Science.gov (United States)

    Holland, Fiona; Archer, Stephanie; Montague, Jane

    2016-08-01

    Most women do not reconstruct their breast(s) post-mastectomy. The experiences of younger women who maintain this decision, although important to understand, are largely absent in the research literature. This interview-based study uses interpretative phenomenological analysis to explore the experiences of six women, diagnosed with primary breast cancer in their 30s/40s, who decided against delayed reconstruction. Findings reported here focus on one superordinate theme (decision-making) from a larger analysis, illustrating that the women's drive to survive clearly influenced their initial decision-making process. Their tenacity in maintaining their decision is highlighted, despite non-reconstruction sometimes being presented negatively by medical teams. Patient-centred support recommendations are made. PMID:25516557

  6. Analysis of Amphiphilic Lipids and Hydrophobic Proteins Using Nonresonant Femtosecond Laser Vaporization with Electrospray Post-Ionization

    Science.gov (United States)

    Brady, John J.; Judge, Elizabeth J.; Levis, Robert J.

    2011-04-01

    Amphiphilic lipids and hydrophobic proteins are vaporized at atmospheric pressure using nonresonant 70 femtosecond (fs) laser pulses followed by electrospray post-ionization prior to being transferred into a time-of-flight mass spectrometer for mass analysis. Measurements of molecules on metal and transparent dielectric surfaces indicate that vaporization occurs through a nonthermal mechanism. The molecules analyzed include the lipids 1-monooleoyl-rac-glycerol, 1,2-dihexanoyl- sn-glycero-3-phosphocholine, 1,2-dimyristoyl- sn-glycero-3-phosphocholine, and the hydrophobic proteins gramicidin A, B, and C. Vaporization of lipids from blood and milk are also presented to demonstrate that lipids in complex systems can be transferred intact into the gas phase for mass analysis.

  7. Laser post-ionization secondary neutral mass spectrometry for ultra-trace analysis of samples from space return missions

    Science.gov (United States)

    Veryovkin, Igor V.; Calaway, Wallis F.; Emil Tripa, C.; Moore, Jerry F.; Wucher, Andreas; Pellin, Michael J.

    2005-12-01

    A new generation of secondary neutral mass spectrometry (SNMS) instruments has been developed that is especially designed for laser post-ionization (LPI). These instruments combine high useful yield and high background discrimination. Results presented here demonstrate that these instruments can detect one in every four atoms removed from a samples surface - a greater than one order of magnitude improvement over current large frame secondary ion mass spectrometry instruments. Because of their high sensitivity, these new LPI-SNMS instruments are especially amenable to analysis of samples of limited size and rare one-of-a-kind samples. Such an application is analysis of samples returned to Earth from space by the Genesis and Stardust Discovery missions of NASA.

  8. Endosymbiotic gene transfer from prokaryotic pangenomes: Inherited chimerism in eukaryotes.

    Science.gov (United States)

    Ku, Chuan; Nelson-Sathi, Shijulal; Roettger, Mayo; Garg, Sriram; Hazkani-Covo, Einat; Martin, William F

    2015-08-18

    Endosymbiotic theory in eukaryotic-cell evolution rests upon a foundation of three cornerstone partners--the plastid (a cyanobacterium), the mitochondrion (a proteobacterium), and its host (an archaeon)--and carries a corollary that, over time, the majority of genes once present in the organelle genomes were relinquished to the chromosomes of the host (endosymbiotic gene transfer). However, notwithstanding eukaryote-specific gene inventions, single-gene phylogenies have never traced eukaryotic genes to three single prokaryotic sources, an issue that hinges crucially upon factors influencing phylogenetic inference. In the age of genomes, single-gene trees, once used to test the predictions of endosymbiotic theory, now spawn new theories that stand to eventually replace endosymbiotic theory with descriptive, gene tree-based variants featuring supernumerary symbionts: prokaryotic partners distinct from the cornerstone trio and whose existence is inferred solely from single-gene trees. We reason that the endosymbiotic ancestors of mitochondria and chloroplasts brought into the eukaryotic--and plant and algal--lineage a genome-sized sample of genes from the proteobacterial and cyanobacterial pangenomes of their respective day and that, even if molecular phylogeny were artifact-free, sampling prokaryotic pangenomes through endosymbiotic gene transfer would lead to inherited chimerism. Recombination in prokaryotes (transduction, conjugation, transformation) differs from recombination in eukaryotes (sex). Prokaryotic recombination leads to pangenomes, and eukaryotic recombination leads to vertical inheritance. Viewed from the perspective of endosymbiotic theory, the critical transition at the eukaryote origin that allowed escape from Muller's ratchet--the origin of eukaryotic recombination, or sex--might have required surprisingly little evolutionary innovation. PMID:25733873

  9. Chimeric mouse-human IgG1 antibody that can mediate lysis of cancer cells

    International Nuclear Information System (INIS)

    A chimeric mouse-human antibody has been created that recognizes an antigen found on the surface of cells from many carcinomas. Immunoglobulin constant (C) domains of the mouse monoclonal antibody L6, C/sub γ2a/ and C/sub kappa/, were substituted by the human C/sub γ1/ and C/sub kappa/ by recombining cDNA modules encoding variable or C domains. The cDNA constructs were transfected into lymphoid cells for antibody production. The chimeric antibody and mouse L6 antibody bound to carcinoma cells with equal affinity and mediated complement-dependent cytolysis. In the presence of human effector cells, the chimeric antibody gave antibody-dependent cellular cytotoxicity at 100 times lower concentration than that needed for the mouse L6 antibody. The assay for lysis was carried out with 51Cr-labeled target calls. The chimeric antibody, but not the mouse L6 antibody, is effective against a melanoma line expressing small amounts of the L6 antigen. The findings point to the usefulness of the chimeric antibody approach for obtaining agents with strong antitumor activity for possible therapeutic use in man

  10. Recognition of base pair inversions in duplex by chimeric (alpha,beta) triplex-forming oligonucleotides.

    Science.gov (United States)

    Timofeev, Edward N; Goryaeva, Baira V; Florentiev, Vladimir L

    2006-10-01

    DNA recognition by triplex-forming oligonucleotides (TFOs) is usually limited by homopurine-homopyrimidine sequence in duplexes. Modifications of the third strand may overcome this limitation. Chimeric alpha-beta TFOs are expected to form triplex DNA upon binding to non-regular sequence duplexes. In the present study we describe binding properties of chimeric alpha-beta oligodeoxynucleotides in the respect to short DNA duplexes with one, three, and five base pair inversions. Non-natural chimeric TFO's contained alpha-thymidine residues inside (GT) or (GA) core sequences. Modified residues were addressed to AT/TA inversions in duplexes. It was found in the non-denaturing gel-electrophoresis experiments that single or five adjacent base pair inversions in duplexes may be recognized by chimeric alpha-beta TFO's at 10 degrees C and pH 7.8. Three dispersed base pair inversions in the double stranded DNA prevented triplex formation by either (GT) or (GA) chimeras. Estimation of thermal stability of chimeric alpha-beta triplexes showed decrease in T(m) values as compared with unmodified complexes. PMID:16928141

  11. Chimeric spider silk proteins mediated by intein result in artificial hybrid silks.

    Science.gov (United States)

    Lin, Senzhu; Chen, Gefei; Liu, Xiangqin; Meng, Qing

    2016-07-01

    Hybrid silks hold a great potential as specific biomaterials due to its controlled mechanical properties. To produce fibers with tunable properties, here we firstly made chimeric proteins in vitro, called W2C4CT and W2C8CT, with ligation of MaSp repetitive modules (C) with AcSp modules (W) by intein trans splicing technology from smaller precursors without final yield reduction. Intein mediated chimeric proteins form fibers at a low concentration of 0.4 mg/mL in 50 mM K3 PO4 pH 7.5 just drawn by hand. Hybrid fibers show smoother surface, and also have stronger chemical resistance as compared with fibers from W2CT (W fibers) and mixture of W2CT/C8CT (MHF8 fibers). Fibers from chimeric protein W2C4CT (HFH4) have improved mechanical properties than W fibers; however, with more C modules W2C8CT fibers (HFH8) properties decreased, indicates the length proportion of various modules is very important and should be optimized for fibers with specific properties. Generally, hybrid silks generated via chimeric proteins, which can be simplified by intein trans splicing, has greater potential to produce fibers with tunable properties. Our research shows that intein mediated directional protein ligation is a novel way to make large chimeric spider silk proteins and hybrid silks. © 2016 Wiley Periodicals, Inc. Biopolymers 105: 385-392, 2016. PMID:26948769

  12. Post Fire Safe Shutdown Analysis Using a Fault Tree Logic Model

    International Nuclear Information System (INIS)

    Every nuclear power plant should have its own fire hazard analysis including the fire safe shutdown analysis. A safe shutdown (SSD) analysis is performed to demonstrate the capability of the plant to safely shut down for a fire in any given area. The basic assumption is that there will be fire damage to all cables and equipment located within a common fire area. When evaluating the SSD capabilities of the plant, based on a review of the systems, equipment and cables within each fire area, it should be determined which shutdown paths are either unaffected or least impacted by a postulated fire within the fire area. Instead of seeking a success path for safe shutdown given all cables and equipment damaged by a fire, there can be an alternative approach to determine the SSD capability: fault tree analysis. This paper introduces the methodology for fire SSD analysis using a fault tree logic model

  13. Deep sequencing analysis of small noncoding RNA and mRNA targets of the global post-transcriptional regulator, Hfq

    DEFF Research Database (Denmark)

    Sittka, A; Lucchini, S; Papenfort, K;

    2008-01-01

    Recent advances in high-throughput pyrosequencing (HTPS) technology now allow a thorough analysis of RNA bound to cellular proteins, and, therefore, of post-transcriptional regulons. We used HTPS to discover the Salmonella RNAs that are targeted by the common bacterial Sm-like protein, Hfq. Initial...... transcriptomic analysis revealed that Hfq controls the expression of almost a fifth of all Salmonella genes, including several horizontally acquired pathogenicity islands (SPI-1, -2, -4, -5), two sigma factor regulons, and the flagellar gene cascade. Subsequent HTPS analysis of 350,000 cDNAs, derived from RNA co......-immunoprecipitation (coIP) with epitope-tagged Hfq or control coIP, identified 727 mRNAs that are Hfq-bound in vivo. The cDNA analysis discovered new, small noncoding RNAs (sRNAs) and more than doubled the number of sRNAs known to be expressed in Salmonella to 64; about half of these are associated with Hfq. Our analysis...

  14. Pathology Tissue-quantitative Mass Spectrometry Analysis to Profile Histone Post-translational Modification Patterns in Patient Samples.

    Science.gov (United States)

    Noberini, Roberta; Uggetti, Andrea; Pruneri, Giancarlo; Minucci, Saverio; Bonaldi, Tiziana

    2016-03-01

    Histone post-translational modifications (hPTMs) generate a complex combinatorial code that has been implicated with various pathologies, including cancer. Dissecting such a code in physiological and diseased states may be exploited for epigenetic biomarker discovery, but hPTM analysis in clinical samples has been hindered by technical limitations. Here, we developed a method (PAThology tissue analysis of Histones by Mass Spectrometry - PAT-H-MS) that allows to perform a comprehensive, unbiased and quantitative MS-analysis of hPTM patterns on formalin-fixed paraffin-embedded (FFPE) samples. In pairwise comparisons, histone extracted from formalin-fixed paraffin-embedded tissues showed patterns similar to fresh frozen samples for 24 differentially modified peptides from histone H3. In addition, when coupled with a histone-focused version of the super-SILAC approach, this method allows the accurate quantification of modification changes among breast cancer patient samples. As an initial application of the PAThology tissue analysis of Histones by Mass Spectrometry method, we analyzed breast cancer samples, revealing significant changes in histone H3 methylation patterns among Luminal A-like and Triple Negative disease subtypes. These results pave the way for retrospective epigenetic studies that combine the power of MS-based hPTM analysis with the extensive clinical information associated with formalin-fixed paraffin-embedded archives. PMID:26463340

  15. An analysis of the 'costs and effectiveness' of post-mastectomy local-regional radiation therapy

    International Nuclear Information System (INIS)

    Purpose: To estimate the 'cost/effectiveness' ratio for post-mastectomy local-regional radiation therapy (LR-XRT) for patients with breast cancer. As the risk of local-regional recurrence (LRR) is related to the number of positive axillary lymph nodes, the cost/effectiveness ratio of LR-XRT was assessed for a variable number of positive axillary nodes. For overall survival (OS), the cost per additional life-year is estimated as a function of follow-up duration. Methods/Assumptions: Literature data indicate that the risk of LRR is related to the number of positive axillary nodes (e.g. Fowble, Kuske). For example, the LRR rate is ∼10% for patients with negative nodes, increasing to ∼40% for patients with ≥4 positive nodes. Such data are used to linearly relate the number of positive nodes with the risk of LRR. Additional assumptions include: RT reduces the risk of LRR by 67%; local recurrences are treated with exision/biopsy, LR-XRT and systemic chemotherapy; half of the local recurrences are controlled locally and half will require additional salvage treatment with surgery or chemotherapy. The 5, 10 and 15 yr improvements in OS provided by LR-XRT are estimated to be 3, 7, and 10%, respectively, based on the literature (Overgaard, Ragaz, Klefstrom, Rutqvist). For each year following LR-XRT, the accrued life-years gained are estimated from the published survival curves. Professional and technical charges are used as a surrogate for costs, as follows (in thousands): LR-XRT-15; evaluation of recurrence-2; biopsy plus pathology-1; resection plus pathology-7, chemotherapy-10. Results: The cost per LRR prevented with the addition of routine post-mastectomy LR-XRT is shown, as a function of the number of positive axillary nodes. The cost per additional life-year gained with LR-XRT, as a function of follow-up duration, is shown. With increasing follow-up, the cost per life-year gained decreases as the additional life-years accrue. Conclusions: The cost per LRR

  16. Measurement of G Using a Cryogenic Torsion Pendulum: Post-2008 Analysis Review

    Directory of Open Access Journals (Sweden)

    Newman Riley

    2014-06-01

    Full Text Available A measurement of G by our University of California, Irvine (UCI team was reported at the 2008 Conference on Precision Electromagnetic Measurements (CPEM [1]. This work has not yet been published; work continues to review and refine analysis of data from that measurement. We present in this paper the analysis and results of the measurement as it stood at the time of the 2008 conference, and discuss the analysis review under way. Our G measurement was conducted “blind”, keeping the exact value of the source mass hidden until just before the conference presentation.

  17. The studies of post-medieval glass by multivariate and X-ray fluorescence analysis

    International Nuclear Information System (INIS)

    Multivariate statistical analysis of the results obtained by energy dispersive X-ray fluorescence analysis has been used in the study of baroque vessel glasses originated from central Europe. X-ray spectrometry can be applied as a completely non-destructive, non-sampling and multi-element method. It is very useful in the studies of valuable historical artefacts. For the last years, multivariate statistical analysis has been developed as an important tool for the archaeometric purposes. Cluster, principal component and discriminant analysis were applied for the classification of the examined objects. The obtained results show that these statistical tools are very useful and complementary in the studies of historical objects. (author)

  18. Studying post-stroke functional impairment after treatment with neural progenitor cells – an analysis of behavioral tests

    Directory of Open Access Journals (Sweden)

    Thorsten R. Doeppner

    2014-10-01

    Full Text Available Systemic transplantation of neural progenitor cells (NPCs in rodents reduces functional impairment after cerebral ischemia. In light of upcoming stroke trials regarding safety and feasibility of NPC transplantation, experimental studies have to successfully analyze the extent of NPC-induced neurorestoration on the functional level. However, appropriate behavioral tests for analysis of post-stroke motor coordination deficits and cognitive impairment after NPC grafting are not fully established. We therefore exposed male C57BL6 mice to either 45 min (mild or 90 min (severe of cerebral ischemia, using the thread occlusion model followed by intravenous injection of PBS or NPCs 6 h post-stroke with an observation period of three months. Post-stroke motor coordination was assessed by means of the rota rod, tight rope, corner turn, inclined plane, grip strength, foot fault, adhesive removal, pole test and balance beam test, whereas cognitive impairment was analyzed using the water maze, the open field and the passive avoidance test. Significant motor coordination differences after both mild and severe cerebral ischemia in favor of NPC-treated mice were observed for each motor coordination test except for the inclined plane and the grip strength test, which only showed significant differences after severe cerebral ischemia. Cognitive impairment after mild cerebral ischemia was successfully assessed using the water maze test, the open field and the passive avoidance test. On the contrary, the water maze test was not suitable in the severe cerebral ischemia paradigm, as it too much depends on motor coordination capabilities of test mice. In terms of both reliability and cost-effectiveness considerations, we thus recommend the corner turn, foot fault, balance beam, and open field test, which do not depend on durations of cerebral ischemia.

  19. Dose-response model of murine typhus (Rickettsia typhi: time post inoculation and host age dependency analysis

    Directory of Open Access Journals (Sweden)

    Tamrakar Sushil B

    2012-03-01

    Full Text Available Abstract Background Rickettsia typhi (R. mooseri is the causative agent of murine typhus. It is one of the most widely distributed flea-borne diseases with a relatively mild febrile initial illness with six to 14 days of incubation period. The bacterium is gram negative and an obligate intracellular pathogen. The disease is transmitted to humans and vertebrate host through fleabites or via contact with infected feces. This paper develops dose-response models of different routes of exposure for typhus in rodents. Methods Data from published articles were analyzed using parametric dose-response relationship models. Dose-response relationships were fit to data using the method of maximum likelihood estimation (MLE. Results Dose-response models quantifying the effects of different ages of rats and time post inoculation in BALB/c mice were analyzed in the study. Both the adult rats (inoculated intradermally and newborn rats (inoculated subcutaneously were best fit by exponential models and both distributions could be described by a single dose-response relationship. The BALB/C mice inoculated subcutaneously were best fit by Beta-Poisson models. The time post inoculation analysis showed that there was a definite time and response relationship existed in this case. Conclusions Intradermally or subcutaneously inoculated rats (adult and newborn models suggest that less than 1 plaque-forming unit (PFU (1.33 to 0.38 in 95% confidence limits of the pathogen is enough to seroconvert 50% of the exposed population on average. For the BALB/c mouse time post inoculation model, an average dose of 0.28 plaque-forming units (PFU (0.75 to 0.11 in 95% confidence limits will seroconvert 50% of the exposed mice.

  20. Surface analysis of TFTR vacuum vessel samples subjected to the post-weld heat treatment

    International Nuclear Information System (INIS)

    To ensure the dimensional stability of the Tokamak Fusion Test Reactor (TFTR) vacuum vessel, it is necessary to perform a post-weld heat treatment (PWHT). This process consists of heating the vessel segments to approx. 4500C for 1.5 h. The large size of the segments precludes a vacuum bake previous to installation. Effects of the PWHT on the vacuum vessel surface were studied using small samples of vessel material which were subjected to a variety of PWHT procedures, including inert gas purges and different oven designs. Changes in topography and near-surface chemistry were investigated with SEM and sputter-Auger electron spectroscopy. These samples were compared with the surface properties of non-baked UHV-quality stainless steel. The primary difference noted between the PWHT samples and the non-baked control was the thickness of the passivation oxide layer. The thickness of this mixed oxide (FeO/Cr2O3/NiO) on the control sample was less than or equal to 100 A. The thickness of the oxide layer on the heat-treated samples ranged between 230 to 350 A, depending on the method of the PWHT. The effect of hydrogen glow discharge cleaning on these thicker oxide layers, and the consequences of such heat treatment procedure relative to oxygen impurity production in fusion devices are discussed

  1. Analysis of the infrared spectra of the peculiar post-AGB stars EPLyr and HD52961

    CERN Document Server

    Gielen, C; Matsuura, M; Min, M; Deroo, P; Waters, L B F M; Dominik, C

    2009-01-01

    Aim: We aim to study in detail the peculiar mineralogy and structure of the circumstellar environment of two binary post-AGB stars, EPLyr and HD52961. Both stars were selected from a larger sample of evolved disc sources observed with Spitzer and show unique solid-state and gas features in their infrared spectra. Moreover, they show a very small infrared excess in comparison with the other sample stars. Methods: The different dust and gas species are identified on the basis of high-resolution Spitzer-IRS spectra. We fit the full spectrum to constrain grain sizes and temperature distributions in the discs. This, combined with our broad-band spectral energy distribution and interferometric measurements, allows us to study the physical structure of the disc, using a self-consistent 2D radiative-transfer disc model. Results: We find that both stars have strong emission features due to CO_2 gas, dominated by ^{12}C^{16}O_2, but with clear ^{13}C^{16}O_2 and even ^{16}O^{12}C^{18}O isotopic signatures. Crystalline ...

  2. Profitability Determinants in Indian Drugs and Pharmaceutical Industry: An Analysis of Pre and Post TRIPS Period

    Directory of Open Access Journals (Sweden)

    Shilpi TYAGI

    2016-05-01

    Full Text Available This paper analyses the determinants of profitability of the Indian drug and pharmaceutical industry, which is known for historically weak innovative R&D initiatives. The change in the economic environment brought out by the TRIPS compliance, this industry was found to have fast adjusted to new working environment by substantially modifying its strategies. The study uses inflation adjusted time-series data for a period 1990-2014 and applies OLS regression model with Newey-West standard errors. It has found that export intensity, A&M intensity, and post-product patent regime have exercised positive influence on industries’ profitability. The negative and statistically significant influence of Leverage ratio and operating expenditure to total assets ratio points to the need for firms to better their efficient management of funds, and contain costs. The study suggests that firms are required to pay far more attention to optimize their operating expenditures, advertisement and marketing expenditures and improve their export orientation, as part of the long term strategy.

  3. Markov chain Monte Carlo based analysis of post-translationally modified VDAC1 gating kinetics

    Directory of Open Access Journals (Sweden)

    Shivendra eTewari

    2015-01-01

    Full Text Available The voltage-dependent anion channel (VDAC is the main conduit for permeation of solutes (including nucleotides and metabolites of up to 5 kDa across the mitochondrial outer membrane (MOM. Recent studies suggest that VDAC activity is regulated via post-translational modifications (PTMs. Yet the nature and effect of these modifications is not understood. Herein, single channel currents of wild-type, nitrosated and phosphorylated VDAC are analyzed using a generalized continuous-time Markov chain Monte Carlo (MCMC method. This developed method describes three distinct conducting states (open, half-open, and closed of VDAC1 activity. Lipid bilayer experiments are also performed to record single VDAC activity under un-phosphorylated and phosphorylated conditions, and are analyzed using the developed stochastic search method. Experimental data show significant alteration in VDAC gating kinetics and conductance as a result of PTMs. The effect of PTMs on VDAC kinetics is captured in the parameters associated with the identified Markov model. Stationary distributions of the Markov model suggests that nitrosation of VDAC not only decreased its conductance but also significantly locked VDAC in a closed state. On the other hand, stationary distributions of the model associated with un-phosphorylated and phosphorylated VDAC suggest a reversal in channel conformation from relatively closed state to an open state. Model analyses of the nitrosated data suggest that faster reaction of nitric oxide with Cys-127 thiol group might be responsible for the biphasic effect of nitric oxide on basal VDAC conductance.

  4. Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein

    KAUST Repository

    Mahfouz, Magdy M.

    2011-12-14

    Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3. SRDX protein efficiently repressed the transcription of the RD29A

  5. The determination of lymphoid cell chimerism using peripheral blood lymphocytes from murine bone marrow chimeras

    International Nuclear Information System (INIS)

    A simple, rapid and accurate method was devised for determining lymphoid cell chimerism in bone marrow-reconstituted mice. Chimeras were produced by reconstituting lethally irradiated mice with semi-allogeneic bone marrow cells. Lymphocytes from the peripheral blood of individual chimeric mice were purified by sedimentation in dextran solution and differential flotation in Ficoll-Hypaque gradients. From 250-500 μl of blood, 1-7 x 105 cells were routinely obtained. The extent of chimerism was determined serologically by using peripheral blood lymphocytes as target cells in a dye exclusion microcytotoxicity assay. Using this new technique, approximately 80% of the reconstituted mice were found to be repopulated with lymphocytes of the donor type. (Auth.)

  6. [Genetic transformation of flax (Linum usitatissimum L.) with chimeric GFP-TUA6 gene for visualisation of microtubules].

    Science.gov (United States)

    Shisha, E N; Korkhovoĭ, V I; Baer, G Ia; Guzenko, E V; Lemesh, V A; Kartel', N A; Emets, A I; Blium, Ia B

    2013-01-01

    The data of Agrobacterium-mediated transformation of some Linum usitatissimum cultivars zoned on the territories of Belarus and Ukraine with the plasmid carrying chimeric GFP-TUA6 gene and nptII gene as selectable marker conferring resistance to kanamycin are presented in this study. Transformation was affected by a number of factors including optical density (OD600), time of inoculation of explants with Agrobacterium and co-culture conditions. Transgenic nature of obtained lines was confirmed by PCR analysis. Expression of GFP-TUA6 gene was detected with confocal laser scanning microscopy. The obtained transgenic lines can be used for further functional studies the role of microtubules in the processes of building the flax fibres and resistance to wind. PMID:23745358

  7. The role of human error in risk analysis: Application to pre- and post-maintenance procedures of process facilities

    International Nuclear Information System (INIS)

    Human factors play an important role in the safe operation of a facility. Human factors include the systematic application of information about human characteristics and behavior to increase the safety of a process system. A significant proportion of human errors occur during the maintenance phase. However, the quantification of human error probabilities in the maintenance phase has not been given the amount of attention it deserves. This paper focuses on a human factors analysis in pre-and post- pump maintenance operations. The procedures for removing process equipment from service (pre-maintenance) and returning the equipment to service (post-maintenance) are considered for possible failure scenarios. For each scenario, human error probability is calculated for each activity using the Success Likelihood Index Method (SLIM). Consequences are also assessed in this methodology. The risk assessment is conducted for each component and the overall risk is estimated by adding individual risks. The present study is aimed at highlighting the importance of considering human error in quantitative risk analyses. The developed methodology has been applied to a case study of an offshore process facility

  8. Association between Soy Isoflavone Intake and Breast Cancer Risk for Pre- and Post-Menopausal Women: A Meta-Analysis of Epidemiological Studies

    OpenAIRE

    Meinan Chen; Yanhua Rao; Yi Zheng; Shiqing Wei; Ye Li; Tong Guo; Ping Yin

    2014-01-01

    BACKGROUND: Conclusions drawn from meta-analyses on the association between soy isoflavone intake and breast cancer risk for pre- and post-menopausal women are not fully consistent. These meta-analyses did not explore the influence of different study designs on the pooled results on the basis of distinguishing between pre- and post-menopausal women. METHODOLOGY AND PRINCIPAL FINDINGS: We performed a meta-analysis of 35 studies which reported results of association between soy isoflavone intak...

  9. Spectral mixture analysis to assess post-fire vegetation regeneration using Landsat Thematic Mapper imagery: Accounting for soil brightness variation

    Science.gov (United States)

    Veraverbeke, S.; Somers, B.; Gitas, I.; Katagis, T.; Polychronaki, A.; Goossens, R.

    2012-02-01

    Post-fire vegetation cover is a crucial parameter in rangeland management. This study aims to assess the post-fire vegetation recovery 3 years after the large 2007 Peloponnese (Greece) wildfires. Post-fire recovery landscapes typically are mixed vegetation-substrate environments which makes spectral mixture analysis (SMA) a very effective tool to derive fractional vegetation cover maps. Using a combination of field and simulation techniques this study aimed to account for the impact of background brightness variability on SMA model performance. The field data consisted out of a spectral library of in situ measured reflectance signals of vegetation and substrate and 78 line transect plots. In addition, a Landsat Thematic Mapper (TM) scene was employed in the study. A simple SMA, in which each constituting terrain feature is represented by its mean spectral signature, a multiple endmember SMA (MESMA) and a segmented SMA, which accounts for soil brightness variations by forcing the substrate endmember choice based on ancillary data (lithological map), were applied. In the study area two main spectrally different lithological units were present: relatively bright limestone and relatively dark flysch (sand-siltstone). Although the simple SMA model resulted in reasonable regression fits for the flysch and limestones subsets separately (coefficient of determination R2 of respectively 0.67 and 0.72 between field and TM data), the performance of the regression model on the pooled dataset was considerably weaker ( R2 = 0.65). Moreover, the regression lines significantly diverged among the different subsets leading to systematic over-or underestimations of the vegetative fraction depending on the substrate type. MESMA did not solve the endmember variability issue. The MESMA model did not manage to select the proper substrate spectrum on a reliable basis due to the lack of shape differences between the flysch and limestone spectra,. The segmented SMA model which accounts for

  10. Analysis of residual stress relief mechanisms in post-weld heat treatment

    International Nuclear Information System (INIS)

    This paper presents a recent study on weld residual stress relief mechanisms associated with furnace-based uniform post-weld heat treatment (PWHT). Both finite element and analytical methods are used to quantitatively examine how plastic deformation and creep relaxation contribute to residual stress relief process at different stages of PWHT process. The key contribution of this work to an improved understanding of furnace based uniform PWHT can be summarized as follows: (1)Plastic deformation induced stress relief during PWHT can be analytically expressed by the change in material elastic deformation capacity (or elastic deformation limit) measured in terms of material yield strength to Young's modulus ratio, which has a rather limited role in overall residual stress relief during furnace based uniform PWHT. (2)The most dominant stress relief mechanism is creep strain induced stress relaxation, as expected. However, a rapid creep strain development accompanied by a rapid residual stress reduction during heating stage before reaching PWHT temperature is shown to contribute to most of the stress relief seen in overall PWHT process, suggesting PWHT hold time can be significantly reduced as far as residual stress relief is concerned. (3)A simple engineering scheme for estimating residual stress reduction is proposed based on this study by relating material type, PWHT temperature, and component wall thickness. - Highlights: • The paper clarified effects of plastic deformation and creep relaxation on weld residual stress relief during uniform PWHT. • Creep strain development is far more important than plastic strain, mostly completed even before hold time starts. • Plastic strain development is insignificant and be analytically described by a material elastic deformation capacity parameter. • An engineering estimation scheme is proposed for determining residual stress reduction resulted from furnace based PWHT

  11. Post-Colonial Analysis of Joseph Conrad’s Heart of Darkness

    Directory of Open Access Journals (Sweden)

    Samet Güven

    2013-06-01

    Full Text Available Joseph Conrad’s Heart of Darkness tells the journey of Marlow through the African jungle and his search for the European Kurtz who exploits the natives by imposing violence. It is mainly based upon Conrad’s own experience in Congo. Conrad learned how Europeans exploited and traded the natives for their own benefits during his own journey. The book is regarded as an attack on imperialism and criticizes immoral treatments of the European colonizers in Africa in the 19th century. Keith Booker states that “the book deals with issues such as imperialism, capitalism, race, and gender that were very much at the forefront of the turn-of-the century European mind. Conrad’s ambivalent treatment of these issues is extremely representative of the way they were treated in any number of European discourses of the time” (217. Besides, Chinua Achebe in his An “Image of Africa: Racism in Conrad’s Heart of Darkness” comments that “Heart of Darkness projects the image of Africa as ‘the other world’, the antithesis of Europe and therefore of civilization, a place where man’s vaunted intelligence and refinement are finally mocked by triumphant bestiality” (338. Although Achebe puts forward that the novel is in favor of colonialism, I consider that Conrad does not intend to write it to appreciate colonialism and therefore the purpose of this study is to approach Conrad’s Heart of Darkness from post-colonial perspective by taking European imperialism and colonialism over Africa into consideration in order to clarify how Conrad has deconstructed binary oppositions of colonialism by subverting the general idea of the Europeans towards Africa in the 19th century.

  12. Post-Colonial Analysis of Joseph Conrad’s Heart of Darkness

    Directory of Open Access Journals (Sweden)

    Samet Güven

    2013-07-01

    Full Text Available Joseph Conrad’s Heart of Darkness tells the journey of Marlow through the African jungle and his search for the European Kurtz who exploits the natives by imposing violence. It is mainly based upon Conrad’s own experience in Congo. Conrad learned how Europeans exploited and traded the natives for their own benefits during his own journey. The book is regarded as an attack on imperialism and criticizes immoral treatments of the European colonizers in Africa in the 19th century. Keith Booker states that “the book deals with issues such as imperialism, capitalism, race, and gender that were very much at the forefront of the turn-of-the century European mind. Conrad’s ambivalent treatment of these issues is extremely representative of the way they were treated in any number of European discourses of the time” (217. Besides, Chinua Achebe in his An “Image of Africa: Racism in Conrad’s Heart of Darkness” comments that “Heart of Darkness projects the image of Africa as ‘the other world’, the antithesis of Europe and therefore of civilization, a place where man’s vaunted intelligence and refinement are finally mocked by triumphant bestiality” (338. Although Achebe puts forward that the novel is in favor of colonialism, I consider that Conrad does not intend to write it to appreciate colonialism and therefore the purpose of this study is to approach Conrad’s Heart of Darkness from post-colonial perspective by taking European imperialism and colonialism over Africa into consideration in order to clarify how Conrad has deconstructed binary oppositions of colonialism by subverting the general idea of the Europeans towards Africa in the 19th century.

  13. Preliminary basin analysis of late Proterozoic-Cambrian post-rift strata, southeast Idaho thrust belt

    Energy Technology Data Exchange (ETDEWEB)

    Link, P.K.; Jansen, S.T.; Halimdihardja, P.; Lande, A.C.; Zahn, P.D.

    1987-08-01

    Strata of the Brigham Group in the Paris-Putnam plate of the southeastern Idaho thrust belt span the late Proterozoic-Cambrian boundary and consist of quartzose sandstone with subordinate pebble conglomerate and siltstone. The Brigham Group is overlain by fossiliferous Cambrian carbonate units that represent the transition from siliciclastic to carbonate deposition in the Cordilleran miogeocline. The Brigham Group contains four stratigraphic sequences bounded by regional disconformities. The lower sequence includes strata below the Brigham group (upper member, Pocatello Formation), plus the Papoose Creek Formation and most of the overlying Caddy Canyon Quartzite. This sequence is dominantly marine with shoreface and braided fluvial strata at the top. The first sequence is overlain disconformably by offshore sub-wave base marine strata of the upper Caddy Canyon Quartzite and Inkom Formation. This second sequence is entirely marine and is composed dominantly of siltstone with sandstone-filled channels. The third sequence comprises the Mutual Formation, an entirely braided fluvial and lacustrine unit. The fourth sequence (Sauk sequence) locally overlies the Mutual Formation with an erosional unconformity and consists of dominantly marine strata of the Camelback Mountain Quartzite, Gibson Jack Formation, Windy Pass Argillite, Twin Knobs Formation, and Sedgwick peak Quartzite. Correlations of these sequences to the McCoy Creek Group of eastern Nevada suggests uniform conditions of sea level and subsidence across the late Proterozoic-Cambrian Cordilleran miogeocline. This preliminary synthesis suggests the Brigham and McCoy Creek Groups are post-rift deposits, as indicated by regional persistence of facies, paleocurrents, and quartzose petrology.

  14. Meaningful-Experience Creation and Event Management A Post-Event Analysis of Copenhagen Carnival 2009

    Directory of Open Access Journals (Sweden)

    Sarah Holst Kjær

    2011-06-01

    Full Text Available A carnival is a cultural event within the experience economy, and can be considered an activity of added value to a city when creating place-awareness for tourists and residents. ‘Culture’ is used as a way to regenerate post-industrial and run down places, when studying EU – as well as Nordic – cultural policy reports. This might be too much to expect from the cultural sector though. Amongst other external factors, cultural policy ideals co-create and affect the experiential content of an event in various ways. Thus studying a carnival one has to include external and internal factors in order to evaluate their meaningfulness in the total experience of the event. One way to investigate what a meaningful experience is can be to apply a cultural consumer perspective. How different consumer segments directly and indirectly inform the event organisation and how the consumer’s cultural preconceptions judge the event is vital when an event organisation designs and improves its experience concepts and experience setting. Thus, the way the carnival’s venue and activities are culturally received is closely linked to the management of the organisation’s external and internal resources. The goal of an event organisation is to produce meaningful and appealing experience concepts and perform them in real time. But how is this organised in practice? This article evaluates the production of the Copenhagen Carnival 2009 and is based on ethnographic material. Through a model of Value Framework for Experience Production by the Dutch experience economists Albert Boswijk, Thomas Thijssen & Ed Peelen (2007 I analyse how the practical organisation, technical solutions and cultural assumptions of a carnival are part of an event organisation’s work-process when creating a spectacle. Furthermore, the organisation of voluntary professional culture workers and the navigation in a metropolitan, political and institutional context is examined through the

  15. Boosting BCG-primed mice with chimeric DNA vaccine HG856A induces potent multifunctional T cell responses and enhanced protection against Mycobacterium tuberculosis.

    Science.gov (United States)

    Ji, Ping; Hu, Zhi-Dong; Kang, Han; Yuan, Qin; Ma, Hui; Wen, Han-Li; Wu, Juan; Li, Zhong-Ming; Lowrie, Douglas B; Fan, Xiao-Yong

    2016-02-01

    The tuberculosis pandemic continues to rampage despite widespread use of the current Bacillus Calmette-Guerin (BCG) vaccine. Because DNA vaccines can elicit effective antigen-specific immune responses, including potent T cell-mediated immunity, they are promising vehicles for antigen delivery. In a prime-boost approach, they can supplement the inadequate anti-TB immunological memory induced by BCG. Based on this, a chimeric DNA vaccine HG856A encoding Mycobacterium tuberculosis (M. tuberculosis) immunodominant antigen Ag85A plus two copies of ESAT-6 was constructed. Potent humoral immune responses, as well as therapeutic effects induced by this DNA vaccine, were observed previously in M. tuberculosis-infected mice. In this study, we further evaluated the antigen-specific T cell immune responses and showed that repeated immunization with HG856A gave modest protection against M. tuberculosis challenge infection and significantly boosted the immune protection primed by BCG vaccination. Enhanced protection was accompanied by increased multifunctional Th1 CD4(+) T cell responses, most notably by an elevated frequency of M. tuberculosis antigen-specific IL-2-producing CD4(+) T cells post-vaccination. These data confirm the potential of chimeric DNA vaccine HG856A as an anti-TB vaccine candidate. PMID:26111521

  16. Chimerism a natural ability to tolerate kin, evolutionary traits connecting mammalian and protochordates

    Directory of Open Access Journals (Sweden)

    A Voskoboynik

    2009-03-01

    Full Text Available In the middle of the 20th century, Owen (1945, 1954 and Billingham et al. (1953 immunological studies suggested that fetal exposure to foreign antigens during pregnancy induce immunologic tolerance in the fetus. Recently, Mold et al. found that a substantial number of maternal cells crosses the placenta to reside in fetal lymph nodes and induces the development of regulatory T cells (Tregs that suppress fetal anti-maternal immunity. These Tregs cells persist till, at least, early adulthood. This result demonstrates how chimerism induces fetal tolerance to maternal antigens during mammalian pregnancy. Natural chimerism is the coexistence of two or more genomic lineages within the same individual. It is a common phenomenon which can be detected in a wide variety of multi-cellular organisms. In mammals, natural chimerism can be established during pregnancy between the mother and the fetus or between fetuses in a multiple embryos pregnancy. Restriction of natural chimerism mainly to kin is also observed in colonial marine protochordates. In protochordates, like Botryllus schlosseri, natural chimerism can be established through fusion of vasculature, between a parent colony and its progeny or between siblings (adult distinct colonies.The ability to tolerate a partial allogeneic individual and to create a chimeric entity between these colonies is determined by a single, highly polymorphic, fusion/histocompatibility locus (Fu/HC. Colonies that share at least one allele in their Fu/HC locus would fuse upon contact. A pair that does not share any Fu/HC allele would not. In the chimera, cells transmigrate between partners and in some cases, replace the germline and/or the somatic tissues of the host. This genotype replacement is mediated by stem cells (termed somatic/germ cell parasitism. Botryllus colonies propagate asexually through budding, therefore somatic stem cell parasitism in host colonies can induce the development of a partial allogeneic entity

  17. Interspecies chimeric complementation for the generation of functional human tissues and organs in large animal hosts.

    Science.gov (United States)

    Wu, Jun; Izpisua Belmonte, Juan Carlos

    2016-06-01

    The past decade's rapid progress in human pluripotent stem cell (hPSC) research has generated hope for meeting the rising demand of organ donation, which remains the only effective cure for end-stage organ failure, a major cause of death worldwide. Despite the potential, generation of transplantable organs from hPSCs using in vitro differentiation is far-fetched. An in vivo interspecies chimeric complementation strategy relying on chimeric-competent hPSCs and zygote genome editing provides an auspicious alternative for providing unlimited organ source for transplantation. PMID:26820411

  18. Molecular chimerization of Pasteurella haemolytica leukotoxin to interleukin-2: effects on cytokine and antigen function.

    OpenAIRE

    Hughes, H P; Campos, M.; Potter, A A; Babiuk, L. A.

    1992-01-01

    A chimeric recombinant protein composed of the lktA gene product from Pasteurella haemolytica fused to bovine interleukin-2 (IL-2) was made. The LKT-IL-2 chimera was compared with recombinant bovine IL-2 with regard to the ability to induce proliferative responses and LAK cell activity in bovine peripheral blood mononuclear cells in vitro. In both instances, chimerization had no effect on IL-2 activity. Similarly, the LKT component was unaffected in its ability to induce an effective immune r...

  19. Dosimetry of chimeric TNT in lung cancer patients

    International Nuclear Information System (INIS)

    Objective: To assess the irradiated absorptive dose of tumor and main critical organs chimeric tumor necrotic treatment (chTNT). Methods: In 9 lung cancer patients a single intravenous dose of 131I-chTNT (29.6±3.7) MBq/kg was administered. Blood samples were drawn at different time intervals and urine was collected for up to one week. Tissue distribution was followed for up to one week by gamma camera imaging. The geometric mean of the anterior and posterior counts was obtained from selected regions of interest (ROIs) to determine activity within the critical organs after being subtracted the background activity. Counts from thyroid were obtained from anterior images only. A background region was drawn below the thyroid gland to subtract underlying activity in the neck blood vessels. The geometric mean of the counts in the whole body scintigram at 0.5 h after injection was corrected for radioactive decay from the time of injection , this value being taken as 100%ID. The residence times for each critical organ and the remainder of the body were computed by dividing the area under their %ID/h curves by the 100%ID value. Absorbed doses to the whole body and to normal organ were computed from the residence time according to the MIRD scheme using Mirdose-3 software. Absorbed doses to tumor tissues were estimated using the same approach taken for normal organs. S-factors for tumors were estimated by comparison with normal organs of similar mass and position in the body. Results: Mean serum disappearance half time values for 131I-chTNT were α (4.9 ± 9.4) h, β (61.7 ± 21.2) h; and whole body, (99 ± 10) h. Mean urine biological clearance half time values was (90 ± 10) h. The absorbed dose of tumor was (8.28 ± 2.65) Gy, the tumor-to-nontumor ratio was 3.95 ± 1.55, while the absorbed dose of marrow was 0.44-0.73 Gy, thyroid was 0.47-23.09 Gy, ovaries was 0.50-0.77 Gy, testicles was 0.38-0.58 Gy, kidneys was 1.71- 4.55 Gy, liver was 1.18-2.63 Gy, and lungs was 1

  20. Arsenic speciation analysis by post-separation hydride generation and atomic fluorescence detection

    Czech Academy of Sciences Publication Activity Database

    Marschner, Karel; Musil, Stanislav; Rychlovský, P.; Dědina, Jiří

    2014. s. 133-133. [Rio Symposium on Atomic Spectrometry /13./. 19.10.2014-24.10.2014, Merida, Yucatan] R&D Projects: GA ČR GA14-23532S Institutional support: RVO:68081715 Keywords : arsenic speciation analysis * hydride generation * atomic fluorescence spectrometry Subject RIV: CB - Analytical Chemistry, Separation

  1. Strengthening post-graduate educational capacity for health policy and systems research and analysis: the strategy of the Consortium for Health Policy and Systems Analysis in Africa

    OpenAIRE

    Erasmus, E; Lehmann, U.; Agyepong, IA; Alwar, J; de Savigny, D; Kamuzora, P; Mirzoev, T; Nxumalo, N; Tomson, G; Uzochukwu, B; Gilson, L

    2016-01-01

    Background The last 5–10 years have seen significant international momentum build around the field of health policy and systems research and analysis (HPSR + A). Strengthening post-graduate teaching is seen as central to the further development of this field in low- and middle-income countries. However, thus far, there has been little reflection on and documentation of what is taught in this field, how teaching is carried out, educators’ challenges and what future teaching might look like. Me...

  2. Analysis of phytochemical constituents of Eucalyptus citriodora L. responsible for antifungal activity against post-harvest fungi.

    Science.gov (United States)

    Javed, S; Shoaib, A; Mahmood, Z; Mushtaq, S; Iftikhar, S

    2012-01-01

    In vitro antifungal activity and phytochemical constituents of essential oil, aqueous, methanol and chloroform extract of Eucalyptus citriodora Hook leaves were investigated. A qualitative phytochemical analysis was performed for the detection of alkaloids, cardiac glycosides, flavonoids, saponins, sterols, tannins and phenols. Methanolic extract holds all identified biochemical constituents except for the tannin. While these biochemical constituents were found to be absent in essential oil, aqueous and chloroform extracts with the exception of sterols, cardiac glycosides and phenols in essential oil and sterols and phenols in aqueous and chloroform extracts. Antimycotic activity of four fractions of E. citriodora was investigated through agar-well diffusion method against four post-harvest fungi, namely, Aspergillus flavus Link ex Gray, Aspergillus fumigatus Fres., Aspergillus nidulans Eidam ex Win and Aspergillus terreus Thom. The results revealed maximum fungal growth inhibition by methanolic extract (14.5%) followed by essential oil (12.9%), chloroform extract (10.15%) and aqueous extract (10%). PMID:21999598

  3. Validation of SWAT for burnup credit problems by analysis of post irradiation examination of 17*17 PWR fuel assembly

    International Nuclear Information System (INIS)

    For adopting burnup credit in transport or storage of spent fuel (SF), development of a reliable burnup calculation code is crucial. For this purpose, data of Post Irradiation Examination (PIE) have been extensively analyzed to evaluate accuracy of burnup calculation codes for a 14*14 or 15*15 PWR fuel assembly. This study shows results of analysis of this latest PIE with SWAT and ORIGEN2.1. SWAT is an integrated burnup code system for a 17*17 PWR fuel assembly that has been developed by Tohoku University and JAERI. The results show that SWAT can more precisely predict nuclide composition of latest PWR assembly than ORIGEN2.1. (O.M.)

  4. MRI evaluation of post-mastectomy irradiated breast implants: prevalence and analysis of complications

    International Nuclear Information System (INIS)

    Aim: To evaluate the effect of post-mastectomy radiation therapy (RT) on breast implants as detected by magnetic resonance imaging (MRI) searching for short-term complications. Materials and methods: One hundred and forty patients (total of 144 implants) were evaluated by MRI; 80 (group 1) had undergone RT, whereas the remaining 60 patients (group 2) underwent mastectomy with implant reconstruction without RT. Two radiologists evaluated MRI images searching for implant rupture signs, sub-capsular seromas, capsular contracture, soft-tissue oedema, peri-implant fluid collections. Implant ruptures were classified as severe complications; seromas and capsular contractures as moderate complications; oedema and fluid collections as mild complications. The prevalence of MRI findings in the two groups was calculated and compared by unpaired t-test. Cohen's kappa statistics was used to assess interobserver agreement. Results: Sixty-nine out of 144 (48%) implants presented pathological findings at MRI with complication rates of 47.5 and 48.4 for groups 1 and 2, respectively. Two (5%) severe complications, 10 (26%) moderate complications, and 26 (69%) mild complications occurred in group 1 and surgical treatment was performed in 10 cases. Two (6%) severe complications, seven (23%) moderate complications, and 22 (71%) mild complications occurred in group 2 and surgical treatment was performed in eight cases. No significant difference between the two groups was found (p>0.1). Almost perfect agreement between the two radiologists was found for MRI image detection (k=0.86). Conclusion: RT does not seem to cause a significant effect on breast implants in terms of complication rate in patients undergoing implant-based breast reconstruction. One-stage immediate implant-based breast reconstruction performed at the same time as mastectomy could be proposed. -- Highlights: •RT could increase complication rates of implant-based breast reconstruction (IBR). •No general consensus

  5. Developmental regulation of the gene for chimeric calcium/calmodulin-dependent protein kinase in anthers

    Science.gov (United States)

    Poovaiah, B. W.; Xia, M.; Liu, Z.; Wang, W.; Yang, T.; Sathyanarayanan, P. V.; Franceschi, V. R.

    1999-01-01

    Chimeric Ca(2+)/calmodulin-dependent protein kinase (CCaMK) was cloned from developing anthers of lily (Lilium longiflorum Thumb. cv. Nellie White) and tobacco (Nicotiana tabacum L. cv. Xanthi). Previous biochemical characterization and structure/function studies had revealed that CCaMK has dual modes of regulation by Ca(2+) and Ca(2+)/calmodulin. The unique structural features of CCaMK include a catalytic domain, a calmodulin-binding domain, and a neural visinin-like Ca(2+)-binding domain. The existence of these three features in a single polypeptide distinguishes it from other kinases. Western analysis revealed that CCaMK is expressed in a stage-specific manner in developing anthers. Expression of CCaMK was first detected in pollen mother cells and continued to increase, reaching a peak around the tetrad stage of meiosis. Following microsporogenesis, CCaMK expression rapidly decreased and at later stages of microspore development, no expression was detected. A tobacco genomic clone of CCaMK was isolated and transgenic tobacco plants were produced carrying the CCaMK promoter fused to the beta-glucuronidase reporter gene. Both CCaMK mRNA and protein were detected in the pollen sac and their localizations were restricted to the pollen mother cells and tapetal cells. Consistent results showing a stage-specific expression pattern were obtained by beta-glucuronidase analysis, in-situ hybridization and immunolocalization. The stage- and tissue-specific appearance of CCaMK in anthers suggests that it could play a role in sensing transient changes in free Ca(2+) concentration in target cells, thereby controlling developmental events in the anther.

  6. Post-test analysis for the MIDAS DVI tests using MARS

    Energy Technology Data Exchange (ETDEWEB)

    Bae, K. H.; Lee, Y. J.; Kwon, T. S.; Lee, W. J.; Kim, H. C. [KAERI, Taejon (Korea, Republic of)

    2002-05-01

    Various DVI tests have been performed at MIDAS test facility which is a scaled facility of APR1400 applying a modified linear scale ratio. The evaluation results for the various void height tests and direct bypass tests using a multi-dimensional best-estimate analysis code MARS, show that; (a) MARS code has an advanced modeling capability of well predicting major multi-dimensional thermal-hydraulic phenomena occurring in the downcomer, (b) MARS code under-predicts the steam condensation rates, which in turn causes to over-predict the ECC bypass rates. However, the trend of decrease in steam condensation rate and increase in ECC bypass rate in accordance with the increase in steam flow rate, and the calculation results of the ECC bypass rates under the EM analysis conditions generally agree with the test data.

  7. Controllability and flexibility analysis of CO2 post-combustion capture using piperazine and MEA

    DEFF Research Database (Denmark)

    Gaspar, Jozsef; Ricardez-Sandoval, Luis; Jørgensen, John Bagterp;

    2016-01-01

    In this study, we developed a decentralized control scheme and investigate the performance of the piperazine (PZ) and monoethanolamine (MEA) CO2 capture process for industrially-relevant operation scenarios. The base for the design of the control schemes is Relative Gain Array (RGA) analysis comb...... the need for advanced control structures, e.g. MPC, which can explicitly account for constraints in the process variables....

  8. Use of Sentiment Analysis for Capturing Patient Experience From Free-Text Comments Posted Online

    OpenAIRE

    Greaves, Felix; Ramirez-Cano, Daniel; Millett, Christopher; Darzi, Ara; Donaldson, Liam

    2013-01-01

    Background There are large amounts of unstructured, free-text information about quality of health care available on the Internet in blogs, social networks, and on physician rating websites that are not captured in a systematic way. New analytical techniques, such as sentiment analysis, may allow us to understand and use this information more effectively to improve the quality of health care. Objective We attempted to use machine learning to understand patients’ unstructured comments about the...

  9. Private law enforcement in post-communist Southeastern Europe: An economic analysis of arbitration and execution

    OpenAIRE

    Schoenfelder Bruno

    2004-01-01

    The very idea of private law enforcement is alien in Southeastern Europe and appears there to be perceived as somewhat extravagant. A thoroughgoing search for the reasons of an extremely limited scope of the private law enforcement is undertaken in the paper. Private law enforcement is seen as taking three basic forms: self-enforcement, enforcement through other agents and illegal means and through specialized agents and with reliance on legal means. Particularly extensive is the analysis of ...

  10. MRI ANALYSIS OF 3D NORMAL AND POST-GLOSSECTOMY TONGUE MOTION IN SPEECH

    OpenAIRE

    Xing, Fangxu; Murano, Emi Z.; Lee, Junghoon; Woo, Jonghye; Stone, Maureen; Prince, Jerry L.

    2013-01-01

    Measuring the internal muscular motion and deformation of the tongue during natural human speech is of high interest to head and neck surgeons and speech language pathologists. A pipeline for calculating 3D tongue motion from dynamic cine and tagged Magnetic Resonance (MR) images during speech has been developed. This paper presents the result of a complete analysis of eleven subjects’ (seven normal controls and four glossectomy patients) global tongue motion during speech obtained through MR...

  11. The relationship between adult attachment style and post-traumatic stress symptoms: A meta-analysis

    OpenAIRE

    Woodhouse, S.; Ayers, S; Field, A. P.

    2015-01-01

    There is increasing evidence that adult attachment plays a role in the development and perseverance of symptoms of posttraumatic stress disorder (PTSD). This meta-analysis aims to synthesise this evidence and investigate the relationship between adult attachment styles and PTSD symptoms. A random-effects model was used to analyse 46 studies (N = 9268) across a wide range of traumas. Results revealed a medium association between secure attachment and lower PTSD symptoms (ρ =-.27), and a medium...

  12. A contribution to the study of post-depositional alterations of pottery using TL dating analysis

    OpenAIRE

    Zacharias, N.; Schwedt, A.; Buxeda i Garrigós, Jaume; Michael, C.T.; Mommsen, H.; Kilikoglou, V.

    2007-01-01

    In luminescence dating, the potassium concentration significantly contributes to the dose rate value in the age estimation. Within this study, fine-grain thermoluminescence dating has been applied on sherds of calcareous pottery of known age, excavated at a Roman site in Mallorca, Spain. For those of the samples that showed signs of severe potassium leaching, according to chemical and mineralogical examination, the thermoluminescence analysis provided overestimated dates. By using the known a...

  13. Post flight analysis of NASA standard star trackers recovered from the solar maximum mission

    Science.gov (United States)

    Newman, P.

    1985-01-01

    The flight hardware returned after the Solar Maximum Mission Repair Mission was analyzed to determine the effects of 4 years in space. The NASA Standard Star Tracker would be a good candidate for such analysis because it is moderately complex and had a very elaborate calibration during the acceptance procedure. However, the recovery process extensively damaged the cathode of the image dissector detector making proper operation of the tracker and a comparison with preflight characteristics impossible. Otherwise, the tracker functioned nominally during testing.

  14. Post-test thermal-hydraulic analysis of two intermediate LOCA tests at the ROSA facility including uncertainty evaluation

    International Nuclear Information System (INIS)

    The OECD/NEA ROSA-2 project aims at addressing thermal-hydraulic safety issues relevant for light water reactors by building up an experimental database at the ROSA Large Scale Test Facility (LSTF). The ROSA facility simulates a PWR Westinghouse design with a four-loop configuration and a nominal power of 3423 MWth. Two intermediate break loss-of-coolant-accident (LOCA) experiments (Tests 1 and 2) have been carried out during 2010. The two tests were analyzed by using the US-NRC TRACE best estimate code, employing the same nodalization previously used for the simulation of small-break LOCA experiments of the ROSA-1 programme. A post-test calculation was performed for each test along with uncertainty analysis providing uncertainty bands for each relevant time trend. Uncertainties in the code modelling capabilities as well as in the initial and boundary conditions were taken into account, following the guidelines and lessons learnt through participation in the OECD/NEA BEMUSE programme. Two different versions of the TRACE code were used in the analysis, providing a qualitatively good prediction of the tests. However, the uncertainty analysis revealed differences between the performances of some models in the two versions. The most relevant parameters of the two experimental tests were falling within the computed uncertainty bands

  15. Oocyte vitrification in the 21st century and post-warming fertility outcomes: a systematic review and meta-analysis.

    Science.gov (United States)

    Potdar, Neelam; Gelbaya, Tarek A; Nardo, Luciano G

    2014-08-01

    Oocyte cryopreservation is a rapidly developing technology, which is increasingly being used for various medical, legal and social reasons. There are inconsistencies in information regarding survival rate and fertility outcomes. This systematic review and meta-analysis provides evidence-based information about oocyte survival and fertility outcomes post warming to help women to make informed choices. All randomized and non-randomized, controlled and prospective cohort studies using oocyte vitrification were included. The primary outcome measure was ongoing pregnancy rate/warmed oocyte. Sensitivity analysis for donor and non-donor oocyte studies was performed. Proportional meta-analysis of 17 studies, using a random-effects model, showed pooled ongoing pregnancy and clinical pregnancy rates per warmed oocyte of 7%. Oocyte survival, fertilization, cleavage, clinical pregnancy and ongoing pregnancy rates per warmed oocyte were higher in donor versus non-donor studies. Comparing vitrified with fresh oocytes, no statistically significant difference was observed in fertilization, cleavage and clinical pregnancy rates, but ongoing pregnancy rate was reduced in the vitrified group (odds ratio 0.74), with heterogeneity between studies. Considering the age of women and the reason for cryopreservation, reasonable information can be given to help women to make informed choices. Future studies with outcomes from oocytes cryopreserved for gonadotoxic treatment may provide more insight. PMID:24931362

  16. Post-Flight Analysis of the Guidance, Navigation, and Control Performance During Orion Exploration Flight Test 1

    Science.gov (United States)

    Barth, Andrew; Mamich, Harvey; Hoelscher, Brian

    2015-01-01

    The first test flight of the Orion Multi-Purpose Crew Vehicle presented additional challenges for guidance, navigation and control as compared to a typical re-entry from the International Space Station or other Low Earth Orbit. An elevated re-entry velocity and steeper flight path angle were chosen to achieve aero-thermal flight test objectives. New IMU's, a GPS receiver, and baro altimeters were flight qualified to provide the redundant navigation needed for human space flight. The guidance and control systems must manage the vehicle lift vector in order to deliver the vehicle to a precision, coastal, water landing, while operating within aerodynamic load, reaction control system, and propellant constraints. Extensive pre-flight six degree-of-freedom analysis was performed that showed mission success for the nominal mission as well as in the presence of sensor and effector failures. Post-flight reconstruction analysis of the test flight is presented in this paper to show whether that all performance metrics were met and establish how well the pre-flight analysis predicted the in-flight performance.

  17. Information Analysis Methodology for Border Security Deployment Prioritization and Post Deployment Evaluation

    International Nuclear Information System (INIS)

    Due to international commerce, cross-border conflicts, and corruption, a holistic, information driven, approach to border security is required to best understand how resources should be applied to affect sustainable improvements in border security. The ability to transport goods and people by land, sea, and air across international borders with relative ease for legitimate commercial purposes creates a challenging environment to detect illicit smuggling activities that destabilize national level border security. Smuggling activities operated for profit or smuggling operations driven by cross border conflicts where militant or terrorist organizations facilitate the transport of materials and or extremists to advance a cause add complexity to smuggling interdiction efforts. Border security efforts are further hampered when corruption thwarts interdiction efforts or reduces the effectiveness of technology deployed to enhance border security. These issues necessitate the implementation of a holistic approach to border security that leverages all available data. Large amounts of information found in hundreds of thousands of documents can be compiled to assess national or regional borders to identify variables that influence border security. Location data associated with border topics of interest may be extracted and plotted to better characterize the current border security environment for a given country or region. This baseline assessment enables further analysis, but also documents the initial state of border security that can be used to evaluate progress after border security improvements are made. Then, border security threats are prioritized via a systems analysis approach. Mitigation factors to address risks can be developed and evaluated against inhibiting factor such as corruption. This holistic approach to border security helps address the dynamic smuggling interdiction environment where illicit activities divert to a new location that provides less resistance

  18. Coming to Terms With Permanent Involuntary Childlessness: A Phenomenological Analysis of Bulletin Board Postings

    Directory of Open Access Journals (Sweden)

    Sumaira H. Malik

    2013-02-01

    Full Text Available Little is known about the role that online support communities play in the lives of women faced with permanent involuntary childlessness. To understand the experiences of these women, this study conducted a thematic analysis of messages downloaded from an online community for permanent involuntary childlessness. Four central themes were identified: Feeling like an “outsider”, A whole lifetime of loss, Coming to terms with childlessness and Finding a safe haven online. These findings show that the online community appeared to empower women to move on with their lives and discover a new sense of self-worth and identity beyond that of motherhood.

  19. Uni- and multivariate analysis of eight indications for post-operative radiotherapy and their significance for local-regional cure in advanced head and neck cancer

    International Nuclear Information System (INIS)

    Eighty consecutive patients with advanced head and neck cancer were treated with combined therapy of radical surgery and post-operative radiotherapy. Indications for post-operative radiotherapy were pathological staging of the primary tumour (pT3 or pT4), poorly differentiated or undifferentiated carcinoma (G3, G4), tumour thickness, tumour invasion in the surrounding tissues with slender tumour strands and solitary tumour cells, perineural spread, lymphangio-invasive tumour growth, multiple positive neck nodes or extranodal spread, and microscopical irradicality of the surgical margins. The contribution for prognosis of these indications for post-operative radiotherapy were retrospectively calculated in univariate and multivariate analysis. Of all investigated parameters, the mode of tumour invasion and lymphangio-invasive growth were independent prognostic factors. If these unfavourable prognostic signs are present, post-operative radiotherapy has to be intensified to at least curative doses of 66 Gy or more to areas at risk. (author)

  20. Ectopic bone formation cannot occur by hydroxyapatite/β-tricalcium phosphate bioceramics in green fluorescent protein chimeric mice

    International Nuclear Information System (INIS)

    Highlights: ► Firstly, chimeric mouse model could be established successfully by bone marrow transplantation after irradiation. ► Secondly, bone induction can occur in wild-type mice 90 days after implantation, but not occur in chimeric mice. ► Thirdly, destruction of immune function will block osteoinduction by calcium phosphate ceramics. - Abstract: Many studies have shown that calcium phosphate ceramics (CP) have osteoconductive and osteoinductive properties; however, the exact mechanism of bone induction has not yet been reported. This study was performed to investigate if destroying immunological function will influence osteogenesis, to explain the mechanism which is unclear. In this study, twenty C57BL/6 mice were divided into two groups (n = 10), in group 1, a hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) ceramic was implanted into both the left and right leg muscles of each mouse; in group 2, ten mice experienced lethal irradiation, then were injected bone marrow (BM) cells from green fluorescent protein (GFP) transgenic mice by tail veil, after bone marrow transplantation (BMT), heart, liver, spleen, lung, kidney, and muscle were harvested for biological analysis, after the GFP chimera model was established successfully, the same HA/β-TCP ceramic was implanted into both leg muscles of each mouse immediately after irradiation. 45 and 90 days after implantation, the ceramics of the two groups were harvested to perform with hematoxylin and eosin (HE) and immunohistochemistry (IHC) staining; the results showed that there was no bone formation in group 2, while new bone tissues were detected in group 1. Our findings suggest that the BM cell from GFP transgenic mice is a good biomarker and it could set a good platform for chimera model; it also shows that BM cell is one of cell resources of bone induction, and destruction of immune function will impede osteoinduction by CP. Overall, our results may shed light on clear mechanism study of bone

  1. Ectopic bone formation cannot occur by hydroxyapatite/{beta}-tricalcium phosphate bioceramics in green fluorescent protein chimeric mice

    Energy Technology Data Exchange (ETDEWEB)

    Cheng Lijia [Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu (China); Duan Xin [Department of Orthopaedics, Chengdu Second People' s Hospital, Chengdu (China); Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu (China); Xiang Zhou [Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu (China); Shi Yujun; Lu Xiaofeng; Ye Feng [Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu (China); Bu Hong, E-mail: hongbu@scu.edu.cn [Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu (China); Department of Pathology, West China Hospital, Sichuan University, Chengdu (China)

    2012-12-01

    Highlights: Black-Right-Pointing-Pointer Firstly, chimeric mouse model could be established successfully by bone marrow transplantation after irradiation. Black-Right-Pointing-Pointer Secondly, bone induction can occur in wild-type mice 90 days after implantation, but not occur in chimeric mice. Black-Right-Pointing-Pointer Thirdly, destruction of immune function will block osteoinduction by calcium phosphate ceramics. - Abstract: Many studies have shown that calcium phosphate ceramics (CP) have osteoconductive and osteoinductive properties; however, the exact mechanism of bone induction has not yet been reported. This study was performed to investigate if destroying immunological function will influence osteogenesis, to explain the mechanism which is unclear. In this study, twenty C57BL/6 mice were divided into two groups (n = 10), in group 1, a hydroxyapatite/{beta}-tricalcium phosphate (HA/{beta}-TCP) ceramic was implanted into both the left and right leg muscles of each mouse; in group 2, ten mice experienced lethal irradiation, then were injected bone marrow (BM) cells from green fluorescent protein (GFP) transgenic mice by tail veil, after bone marrow transplantation (BMT), heart, liver, spleen, lung, kidney, and muscle were harvested for biological analysis, after the GFP chimera model was established successfully, the same HA/{beta}-TCP ceramic was implanted into both leg muscles of each mouse immediately after irradiation. 45 and 90 days after implantation, the ceramics of the two groups were harvested to perform with hematoxylin and eosin (HE) and immunohistochemistry (IHC) staining; the results showed that there was no bone formation in group 2, while new bone tissues were detected in group 1. Our findings suggest that the BM cell from GFP transgenic mice is a good biomarker and it could set a good platform for chimera model; it also shows that BM cell is one of cell resources of bone induction, and destruction of immune function will impede

  2. Mitochondrial DNA suggests at least 11 origins of parasitism in angiosperms and reveals genomic chimerism in parasitic plants

    Directory of Open Access Journals (Sweden)

    Croom Henrietta B

    2007-12-01

    Full Text Available Abstract Background Some of the most difficult phylogenetic questions in evolutionary biology involve identification of the free-living relatives of parasitic organisms, particularly those of parasitic flowering plants. Consequently, the number of origins of parasitism and the phylogenetic distribution of the heterotrophic lifestyle among angiosperm lineages is unclear. Results Here we report the results of a phylogenetic analysis of 102 species of seed plants designed to infer the position of all haustorial parasitic angiosperm lineages using three mitochondrial genes: atp1, coxI, and matR. Overall, the mtDNA phylogeny agrees with independent studies in terms of non-parasitic plant relationships and reveals at least 11 independent origins of parasitism in angiosperms, eight of which consist entirely of holoparasitic species that lack photosynthetic ability. From these results, it can be inferred that modern-day parasites have disproportionately evolved in certain lineages and that the endoparasitic habit has arisen by convergence in four clades. In addition, reduced taxon, single gene analyses revealed multiple horizontal transfers of atp1 from host to parasite lineage, suggesting that parasites may be important vectors of horizontal gene transfer in angiosperms. Furthermore, in Pilostyles we show evidence for a recent host-to-parasite atp1 transfer based on a chimeric gene sequence that indicates multiple historical xenologous gene acquisitions have occurred in this endoparasite. Finally, the phylogenetic relationships inferred for parasites indicate that the origins of parasitism in angiosperms are strongly correlated with horizontal acquisitions of the invasive coxI group I intron. Conclusion Collectively, these results indicate that the parasitic lifestyle has arisen repeatedly in angiosperm evolutionary history and results in increasing parasite genomic chimerism over time.

  3. A non-LTE abundance analysis of the post-AGB star ROA 5701

    CERN Document Server

    Thompson, H M A; Dufton, P L; Ryans, R S I; Smoker, J V

    2006-01-01

    An analysis of high-resolution Anglo-Australian Telescope (AAT)/ University College London Echelle Spectrograph (UCLES) optical spectra for the ultraviolet (UV)-bright star ROA 5701 in the globular cluster omega Cen (NGC 5139) is performed, using non-local thermodynamic equilibrium (non-LTE) model atmospheres to estimate stellar atmospheric parameters and chemical composition. Abundances are derived for C, N, O, Mg, Si and S, and compared with those found previously by Moehler et al. We find a general metal underabundance relative to young B-type stars, consistent with the average metallicity of the cluster. Our results indicate that ROA 5701 has not undergone a gas-dust separation scenario as previously suggested. However, its abundance pattern does imply that ROA 5701 has evolved off the AGB prior to the onset of the third dredge-up.

  4. Post-Flight Analysis of GPSR Performance During Orion Exploration Flight Test 1

    Science.gov (United States)

    Barker, Lee; Mamich, Harvey; McGregor, John

    2016-01-01

    On 5 December 2014, the first test flight of the Orion Multi-Purpose Crew Vehicle executed a unique and challenging flight profile including an elevated re-entry velocity and steeper flight path angle to envelope lunar re-entry conditions. A new navigation system including a single frequency (L1) GPS receiver was evaluated for use as part of the redundant navigation system required for human space flight. The single frequency receiver was challenged by a highly dynamic flight environment including flight above low Earth orbit, as well as single frequency operation with ionospheric delay present. This paper presents a brief description of the GPS navigation system, an independent analysis of flight telemetry data, and evaluation of the GPSR performance, including evaluation of the ionospheric model employed to supplement the single frequency receiver. Lessons learned and potential improvements will be discussed.

  5. Post-Nonlinear Sparse Component Analysis Using Single-Source Zones and Functional Data Clustering

    CERN Document Server

    Puigt, Matthieu; Mouchtaris, Athanasios

    2012-01-01

    In this paper, we introduce a general extension of linear sparse component analysis (SCA) approaches to postnonlinear (PNL) mixtures. In particular, and contrary to the state-of-art methods, our approaches use a weak sparsity source assumption: we look for tiny temporal zones where only one source is active. We investigate two nonlinear single-source confidence measures, using the mutual information and a local linear tangent space approximation (LTSA). For this latter measure, we derive two extensions of linear single-source measures, respectively based on correlation (LTSA-correlation) and eigenvalues (LTSA-PCA). A second novelty of our approach consists of applying functional data clustering techniques to the scattered observations in the above single-source zones, thus allowing us to accurately estimate them.We first study a classical approach using a B-spline approximation, and then two approaches which locally approximate the nonlinear functions as lines. Finally, we extend our PNL methods to more gener...

  6. Effect of sample digestion, air filter contamination, and post-adsorption on the analysis of trace elements in air particulate matter

    International Nuclear Information System (INIS)

    Inductively coupled plasma atomic emission spectrometry and inductively coupled plasma MS are the major analytical tools for trace elements in environmental matrices, however, the underestimate of certain trace elements in analysis of air particulate matter by these two techniques has long been observed. This has been attributed to incomplete sample digestion. Here, we demonstrate that the combined effects of sample digestion, air filter impurities, and post-adsorption of the analytes contribute to the interference of the analysis. Particular attention should be paid to post-adsorption of analytes onto air filters after acid digestion. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  7. Contact stress analysis of the anterior tibial post in bi-cruciate stabilized and mobile-bearing posterior stabilized total knee arthroplasty designs.

    Science.gov (United States)

    Kuwashima, Umito; Hamai, Satoshi; Okazaki, Ken; Ikebe, Satoru; Higaki, Hidehiko; Mizu-Uchi, Hideki; Akasaki, Yukio; Murakami, Koji; Iwamoto, Yukihide

    2016-07-01

    In posterior-stabilized (PS) total knee arthroplasty (TKA), unexpected wear and fracture of the tibial post due to anterior post impingement have been reported. The purpose of this study was to determine the contact stress on the anterior aspect of the tibial post in four contemporary TKA designs. We evaluated one bi-cruciate stabilized design (Journey II) and three mobile-bearing PS designs (Vanguard RP, PFC Sigma RP, and NexGen LPS Mobile). The contact conditions at the anterior aspect of the tibial post were determined upon application of a posterior force of 100N to individual implants. Each measurement was sequentially performed five times, and the data were compared within and across designs using analysis of variance and a post-hoc test. The contact stress of the Journey II and Vanguard RP was less than the compressive yield stress for polyethylene (10MPa) at all tested flexion angles and degrees of rotation. The PFC Sigma RP did not show anterior tibial post impingement under any experimental conditions. The NexGen LPS Mobile demonstrated bilateral edge loading at the anterior tibial post and exceeded 10MPa of contact stress in some test conditions. Thus, the differences among implants in terms of the dimensions of the femoral anterior cam or intercondylar notch and the anterior aspect of the tibial post in the axial and sagittal planes led to significant differences in contact conditions. The present study helps the surgeon to be more aware that various contact conditions of the anterior aspect of the tibial post can occur in individual TKA designs. PMID:26999701

  8. Research of Neutron Characteristics of ChNPP RBMK-1000 in the Institute for Nuclear research in pre-and post-emergency period: retrospective analysis

    International Nuclear Information System (INIS)

    The paper considers scientific and technical cooperation of the Institute for Nuclear Research of the Academy of Sciences of the Ukrainian SSR and Chornobyl NPP in the pre- and post-emergency period. The research presents the retrospective analysis of the software for RBMK-1000 safety analysis. Attention is paid to the need for safety analysis improvement by implementation of the latest software, and for creation of the scientific and research institute in Ukraine to address the whole spectrum of nuclear challenges

  9. Safety of inhaled glycopyrronium in patients with COPD: a comprehensive analysis of clinical studies and post-marketing data

    Directory of Open Access Journals (Sweden)

    D’Urzo AD

    2015-08-01

    Full Text Available Anthony D D’Urzo,1 Edward M Kerwin,2 Kenneth R Chapman,3 Marc Decramer,4 Robert DiGiovanni,5 Peter D’Andrea,6 Huilin Hu,6 Pankaj Goyal,5 Pablo Altman6 1Department of Family and Community Medicine, University of Toronto, Toronto, ON, Canada; 2Clinical Research Institute of Southern Oregon, PC, Medford, USA; 3Asthma and Airway Centre, University Health Network, Toronto Western Hospital, Toronto, ON, Canada; 4Respiratory Division, University of Leuven, Leuven, Belgium; 5Novartis Pharma AG, Basel, Switzerland; 6Novartis Pharmaceuticals Corporation, East Hanover, NJ, USA Background: Chronic use of inhaled anticholinergics by patients with chronic obstructive pulmonary disease (COPD has raised long-term safety concerns, particularly cardiovascular. Glycopyrronium is a once-daily anticholinergic with greater receptor selectivity than previously available agents. Methods: We assessed the safety of inhaled glycopyrronium using data pooled from two analysis sets, involving six clinical studies and over 4,000 patients with COPD who received one of the following treatments: glycopyrronium 50 µg, placebo (both delivered via the Breezhaler® device, or tiotropium 18 µg (delivered via the HandiHaler® device. Data were pooled from studies that varied in their duration and severity of COPD of the patients (ie, ≤12 weeks duration with patients having moderate or severe COPD; and >1 year duration with patients having severe and very severe COPD. Safety comparisons were made for glycopyrronium vs tiotropium or placebo. Poisson regression was used to assess the relative risk for either active drug or placebo (and between drugs where placebo was not available for assessing the incidence of safety events. During post-marketing surveillance (PMS, safety was assessed by obtaining reports from various sources, and disproportionality scores were computed using EMPIRICA™. In particular, the cardiac safety of glycopyrronium during the post-marketing phase was

  10. Thermostability of multidomain proteins: chimeric mesophilic/thermophilic elongation factors EF-Tu

    Czech Academy of Sciences Publication Activity Database

    Šanderová, Hana; Maloň, Petr; Hůlková, Marta; Jonák, Jiří

    Varšava : FEBS, 2004, s. 7. [FEBS Forum for Young Scientists. Varšava (PL), 24.06.2004-26.06.2004] R&D Projects: GA ČR GA303/02/0689 Institutional research plan: CEZ:AV0Z5052915 Keywords : thermostability * EF-Tu * chimeric protein Subject RIV: EB - Genetics ; Molecular Biology

  11. Thermostability of multidomain proteins: chimeric mesophilic/thermophilic elongation factors EF-Tu

    Czech Academy of Sciences Publication Activity Database

    Šanderová, Hana; Maloň, Petr; Hůlková, Marta; Jonák, Jiří

    Oxford : Blackwell Publishing, 2004, s. 219. [Meeting of the Federation of the European Biochemical Societies /29./. Varšava (PL), 26.06.2004-01.07.2004] R&D Projects: GA ČR GA303/02/0689 Institutional research plan: CEZ:AV0Z5052915 Keywords : thermostability * EF-Tu * chimeric protein Subject RIV: EB - Genetics ; Molecular Biology

  12. Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Jiping Cai

    2008-08-01

    Full Text Available Vascular endothelial cell growth inhibitor (VEGI is a recently identified antiangiogenic cytokine that belongs to the TNF superfamily, and could effectively inhibit endothelial cell proliferation and angiogenesis. Synthetic peptide CTT (CTTHWGFTLC has been found to suppress invasion and migration of both tumor and endothelial cells by potent and selective inhibition of MMP-2 and MMP-9. To prepare chimeric protein VEGI-CTT for more potent antitumor therapy, the recombinant expression vector pET-VEGI-CTT was constructed. This fusion protein was expressed in inclusion bodies in E. coli BL21 (DE3, and was refolded and purified by immobilized metal affinity chromatography using His-tag. Purified VEGI-CTT protein was characterized by proliferation assays of the endothelial cells and casein degradation assay in vitro. The results demonstrated that chimeric protein VEGI-CTT had a potent activity of antiangiogenesis through inhibiting the proliferation of endothelial cells, and could effectively reduce the activity of MMP-2 and MMP-9. The preliminarily in vivo study demonstrated that chimeric protein VEGI-CTT had more potent antitumor activity than VEGI and/or CTT peptide against CA46 human lymphoma xenografts in nude mice. Thus, these facts that are derived from the present study suggest that the chimeric protein VEGI-CTT may be used for tumor therapy in the future.

  13. Trypanosoma cruzi Differentiates and Multiplies within Chimeric Parasitophorous Vacuoles in Macrophages Coinfected with Leishmania amazonensis.

    Science.gov (United States)

    Pessoa, Carina Carraro; Ferreira, Éden Ramalho; Bayer-Santos, Ethel; Rabinovitch, Michel; Mortara, Renato Arruda; Real, Fernando

    2016-05-01

    The trypanosomatids Leishmania amazonensis and Trypanosoma cruzi are excellent models for the study of the cell biology of intracellular protozoan infections. After their uptake by mammalian cells, the parasitic protozoan flagellates L. amazonensis and T. cruzi lodge within acidified parasitophorous vacuoles (PVs). However, whereas L. amazonensis develops in spacious, phagolysosome-like PVs that may enclose numerous parasites, T. cruzi is transiently hosted within smaller vacuoles from which it soon escapes to the host cell cytosol. To investigate if parasite-specific vacuoles are required for the survival and differentiation of T. cruzi, we constructed chimeric vacuoles by infection of L. amazonensis amastigote-infected macrophages with T. cruzi epimastigotes (EPIs) or metacyclic trypomastigotes (MTs). These chimeric vacuoles, easily observed by microscopy, allowed the entry and fate of T. cruzi in L. amazonensis PVs to be dynamically recorded by multidimensional imaging of coinfected cells. We found that although T. cruzi EPIs remained motile and conserved their morphology in chimeric vacuoles, T. cruzi MTs differentiated into amastigote-like forms capable of multiplying. These results demonstrate that the large adaptive vacuoles of L. amazonensis are permissive to T. cruzi survival and differentiation and that noninfective EPIs are spared from destruction within the chimeric PVs. We conclude that T. cruzi differentiation can take place in Leishmania-containing vacuoles, suggesting this occurs prior to their escape into the host cell cytosol. PMID:26975994

  14. Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes.

    Science.gov (United States)

    Hammill, Joanne A; Afsahi, Arya; Bramson, Jonathan L; Helsen, Christopher W

    2016-01-01

    The adoptive transfer of a bolus of tumor-specific T lymphocytes into cancer patients is a promising therapeutic strategy. In one approach, tumor specificity is conferred upon T cells via engineering expression of exogenous receptors, such as chimeric antigen receptors (CARs). Here, we describe the generation and production of both murine and human CAR-engineered T lymphocytes using retroviruses. PMID:27581020

  15. Custom-engineered chimeric foot-and-mouth disease vaccine elicits protective immune responses in pigs

    Science.gov (United States)

    Chimeric foot-and-mouth disease viruses (FMDV) of which the antigenic properties can be readily manipulated is a potentially powerful approach in the control of foot-and-mouth disease (FMD) in sub-Saharan Africa. FMD vaccine application is complicated by the extensive variability of the South Africa...

  16. In Silico Design of a Chimeric Protein Containing Antigenic Fragments of Helicobacter pylori; A Bioinformatic Approach

    Science.gov (United States)

    Mohammad, Nazanin; Karsabet, Mehrnaz Taghipour; Amani, Jafar; Ardjmand, Abolfazl; Zadeh, Mohsen Razavi; Gholi, Mohammad Khalifeh; Saffari, Mahmood; Ghasemi, Amir

    2016-01-01

    Helicobacter pylori is a global health problem which has encouraged scientists to find new ways to diagnose, immunize and eradicate the H. pylori infection. In silico studies are a promising approach to design new chimeric antigen having the immunogenic potential of several antigens. In order to obtain such benefit in H. pylori vaccine study, a chimeric gene containing four fragments of FliD sequence (1-600 bp), UreB (327-334 bp),VacA (744-805 bp) and CagL(51-100 bp) which have a high density of B- and T-cell epitopes was designed. The secondary and tertiary structures of the chimeric protein and other properties such as stability, solubility and antigenicity were analyzed. The in silico results showed that after optimizing for the purpose of expression in Escherichia coli BL21, the solubility and antigenicity of the construct fragments were highly retained. Most regions of the chimeric protein were found to have a high antigenic propensity and surface accessibility. These results would be useful in animal model application and accounted for the development of an epitope-based vaccine against the H. pylori. PMID:27335622

  17. Ligand-mediated negative regulation of a chimeric transmembrane receptor tyrosine phosphatase

    DEFF Research Database (Denmark)

    Desai, D M; Sap, J; Schlessinger, J;

    1993-01-01

    inactivate the EGFR-CD45 chimera in a manner that is dependent on dimerization of the chimeric protein. Inactivation of EGFR-CD45 chimera function results in the loss of TCR signaling, indicating that CD45 function is continuously required for TCR-mediated proximal signaling events. These results suggest...

  18. Alloreactive regulatory T cells allow the generation of mixed chimerism and transplant tolerance

    Directory of Open Access Journals (Sweden)

    Paulina eRuiz

    2015-11-01

    Full Text Available The induction of donor-specific transplant tolerance is one of the main goals of modern immunology. Establishment of a mixed chimerism state in the transplant recipient has proven to be a suitable strategy for the induction of long-term allograft tolerance; however, current experimental recipient preconditioning protocols have many side effects, and are not feasible for use in future therapies. In order to improve the current mixed chimerism induction protocols, we developed a non-myeloablative bone-marrow transplant protocol using retinoic acid induced alloantigen-specific Tregs, clinically available immunosuppressive drugs and lower doses of irradiation. We demonstrate that retinoic acid induced alloantigen-specific Tregs in addition to a non-myeloablative bone-marrow transplant protocol generates stable mixed chimerism and induce tolerance to allogeneic secondary skin allografts in mice. Therefore, the establishment of mixed chimerism through the use of donor-specific Tregs rather than non-specific immunosuppression could have a potential use in organ transplantation.

  19. SAT Type Foot-and-Mouth Disease (FMD) Chimeric Vaccine Elicits Protection in Pigs

    Science.gov (United States)

    The recent development of infectious cDNA clone technology for foot-and-mouth disease (FMD), Southern African Territories (SAT) viruses has provided a valuable tool for genetic and biological characterization of field and laboratory strains. Recombinant chimeric viruses, containing the capsid-coding...

  20. Origination of an X-linked testes chimeric gene by illegitimate recombination in Drosophila.

    Directory of Open Access Journals (Sweden)

    2006-05-01

    Full Text Available The formation of chimeric gene structures provides important routes by which novel proteins and functions are introduced into genomes. Signatures of these events have been identified in organisms from wide phylogenic distributions. However, the ability to characterize the early phases of these evolutionary processes has been difficult due to the ancient age of the genes or to the limitations of strictly computational approaches. While examples involving retrotransposition exist, our understanding of chimeric genes originating via illegitimate recombination is limited to speculations based on ancient genes or transfection experiments. Here we report a case of a young chimeric gene that has originated by illegitimate recombination in Drosophila. This gene was created within the last 2-3 million years, prior to the speciation of Drosophila simulans, Drosophila sechellia, and Drosophila mauritiana. The duplication, which involved the Bällchen gene on Chromosome 3R, was partial, removing substantial 3' coding sequence. Subsequent to the duplication onto the X chromosome, intergenic sequence was recruited into the protein-coding region creating a chimeric peptide with approximately 33 new amino acid residues. In addition, a novel intron-containing 5' UTR and novel 3' UTR evolved. We further found that this new X-linked gene has evolved testes-specific expression. Following speciation of the D. simulans complex, this novel gene evolved lineage-specifically with evidence for positive selection acting along the D. simulans branch.

  1. THE PERSISTENCE OF CHICKEN HERPES AND RETRO VIRAL CHIMERIC MOLECULES UPON IN VIVO PASSAGE

    Science.gov (United States)

    Marek's disease virus, a herpes virus, and avian leucosis virus subgroup J, a retrovirus were used for experimental co-infection of chicks. Two consecutive trials were performed in attempt to evaluate the formation and persistence of chimeric molecules that would indicate retro-viral integration int...

  2. Atomic resolution structure of a chimeric DNA-RNA Z-type duplex in complex with Ba(2+) ions: a case of complicated multi-domain twinning.

    Science.gov (United States)

    Gilski, Miroslaw; Drozdzal, Pawel; Kierzek, Ryszard; Jaskolski, Mariusz

    2016-02-01

    The self-complementary dCrGdCrGdCrG hexanucleotide, in which not only the pyrimidine/purine bases but also the ribo/deoxy sugars alternate along the sequence, was crystallized in the presence of barium cations in the form of a left-handed Z-type duplex. The asymmetric unit of the P21 crystal with a pseudohexagonal lattice contains four chimeric duplexes and 16 partial Ba(2+) sites. The chimeric (DNA-RNA)2 duplexes have novel patterns of hydration and exhibit a high degree of discrete conformational disorder of their sugar-phosphate backbones, which can at least partly be correlated with the fractional occupancies of the barium ions. The crystals of the DNA-RNA chimeric duplex in complex with Ba(2+) ions and also with Sr(2+) ions exhibit complicated twinning, which in combination with structural pseudosymmetry made structure determination difficult. The structure could be successfully solved by molecular replacement in space groups P1 and P21 but not in orthorhombic or higher symmetry and, after scrupulous twinning and packing analysis, was refined in space group P21 to an R and Rfree of 11.36 and 16.91%, respectively, using data extending to 1.09 Å resolution. With the crystal structure having monoclinic symmetry, the sixfold crystal twinning is a combination of threefold and twofold rotations. The paper describes the practical aspects of dealing with cases of complicated twinning and pseudosymmetry, and compares the available software tools for the refinement and analysis of such cases. PMID:26894669

  3. Analysis of the in-pile operation and preliminary results of the post irradiation dismantling of the pebble bed assemblies

    Energy Technology Data Exchange (ETDEWEB)

    Magielsen, A.J.; Peeters, M.M.W.; Hegeman, J.B.J.; Stijkel, M.P.; Laan, J.G. van der [NRG-Nuclear Research and Consultancy Group (Netherlands)

    2007-07-01

    The Pebble Bed Assemblies (PBA) are four tritium breeding sub scale modules, representing a segment of the European Helium Cooled Pebble Bed Test Blanket. The objective of these experiments is the study the thermomechanical behaviour of the pebble bed assemblies during irradiation. This objective will be full- filled by the analysis of changes in the in-pile temperature profiles during irradiation and the post irradiation examination of the pebble beds in the Hot Cells. The PBA has been irradiated in the HFR in Petten for 294 Full Power Days (FPD), to a dose of 2-3 dpa in Eurofer, and estimated lithium burnup of 2-3 %. Changes in the temperature profile during in-pile operation are indication for pebble bed creep compaction during first start up and the possible formation gas gaps between the pebble beds and the structure. During progressive irradiation the radial and axial differential temperatures within the breeder and beryllium pebble beds are evaluated. During start up of the sub sequent irradiation cycles (each 26 FPD) the temperature differences within the beryllium pebble beds show a slight increase suggesting changes in the structure of the pebble beds. The PBA are transported from the HFR to the Hot Cell Laboratory in upright position to maintain the gas gaps between the pebble beds and Eurofer. Various microscopy preparation techniques are used to study the deformation state of the pebble beds (signs of creep compaction and sintering), formation of gas gaps between the pebble beds and structural materials and the interaction layers between eurofer-ceramic and eurofer-beryllium. In this paper first results on the Post Irradiation examination are given. (orig.)

  4. Analysis of deep inferior epigastric perforator (DIEP) arteries by using MDCTA: Comparison between 2 post-processing techniques

    International Nuclear Information System (INIS)

    Purpose: Our purpose was to compare two post-processing techniques, Maximum-Intensity-Projection (MIP) and Volume Rendering (VR) for the study of perforator arteries. Methods: Thirty patients who underwent Multi-Detector-Row CT Angiography (MDCTA) between February 2010 and May 2010 were retrospectively analyzed. For each patient and for each reconstruction method, the image quality was evaluated and the inter- and intra-observer agreement was calculated according to the Cohen statistics. The Hounsfield Unit (HU) value in the common femoral artery was quantified and the correlation (Pearson Statistic) between image quality and HU value was explored. Results: The Pearson r between the right and left common femoral artery was excellent (r = 0.955). The highest image quality score was obtained using MIP for both observers (total value 75, with a mean value 2.67 for observer 1 and total value of 79 and a mean value of 2.82 for observer 2). The highest agreement between the two observers was detected using the MIP protocol with a Cohen kappa value of 0.856. The ROC area under the curve (Az) for the VR is 0.786 (0.086 SD; p value = 0.0009) whereas the ROC area under the curve (Az) for the MIP is 0.0928 (0.051 SD; p value = 0.0001). Conclusion: MIP showed the optimal inter- and intra-observer agreement and the highest quality scores and therefore should be used as post-processing techniques in the analysis of perforating arteries.

  5. Analysis of deep inferior epigastric perforator (DIEP) arteries by using MDCTA: Comparison between 2 post-processing techniques

    Energy Technology Data Exchange (ETDEWEB)

    Saba, Luca, E-mail: lucasaba@tiscali.it [Department of Radiology, Azienda Ospedaliero Universitaria (A.O.U.), di Cagliari - Polo di Monserrato, s.s. 554 Monserrato, Cagliari 09045 (Italy); Atzeni, Matteo; Ribuffo, Diego [Department of Vascular Surgery, Azienda Ospedaliero Universitaria (A.O.U.), di Cagliari - Polo di Monserrato, s.s. 554 Monserrato, Cagliari 09045 (Italy); Mallarini, Giorgio [Department of Radiology, Azienda Ospedaliero Universitaria (A.O.U.), di Cagliari - Polo di Monserrato, s.s. 554 Monserrato, Cagliari 09045 (Italy); Suri, Jasjit S. [Biomedical Technologies Inc., Denver, CO (United States); Idaho State University (Aff.), ID (United States)

    2012-08-15

    Purpose: Our purpose was to compare two post-processing techniques, Maximum-Intensity-Projection (MIP) and Volume Rendering (VR) for the study of perforator arteries. Methods: Thirty patients who underwent Multi-Detector-Row CT Angiography (MDCTA) between February 2010 and May 2010 were retrospectively analyzed. For each patient and for each reconstruction method, the image quality was evaluated and the inter- and intra-observer agreement was calculated according to the Cohen statistics. The Hounsfield Unit (HU) value in the common femoral artery was quantified and the correlation (Pearson Statistic) between image quality and HU value was explored. Results: The Pearson r between the right and left common femoral artery was excellent (r = 0.955). The highest image quality score was obtained using MIP for both observers (total value 75, with a mean value 2.67 for observer 1 and total value of 79 and a mean value of 2.82 for observer 2). The highest agreement between the two observers was detected using the MIP protocol with a Cohen kappa value of 0.856. The ROC area under the curve (Az) for the VR is 0.786 (0.086 SD; p value = 0.0009) whereas the ROC area under the curve (Az) for the MIP is 0.0928 (0.051 SD; p value = 0.0001). Conclusion: MIP showed the optimal inter- and intra-observer agreement and the highest quality scores and therefore should be used as post-processing techniques in the analysis of perforating arteries.

  6. Analysis Methods for Post Occupancy Evaluation of Energy-Use in High Performance Buildings Using Short-Term Monitoring

    Science.gov (United States)

    Singh, Vipul

    2011-12-01

    The green building movement has been an effective catalyst in reducing energy demands of buildings and a large number of 'green' certified buildings have been in operation for several years. Whether these buildings are actually performing as intended, and if not, identifying specific causes for this discrepancy falls into the general realm of post-occupancy evaluation (POE). POE involves evaluating building performance in terms of energy-use, indoor environmental quality, acoustics and water-use; the first aspect i.e. energy-use is addressed in this thesis. Normally, a full year or more of energy-use and weather data is required to determine the actual post-occupancy energy-use of buildings. In many cases, either measured building performance data is not available or the time and cost implications may not make it feasible to invest in monitoring the building for a whole year. Knowledge about the minimum amount of measured data needed to accurately capture the behavior of the building over the entire year can be immensely beneficial. This research identifies simple modeling techniques to determine best time of the year to begin in-situ monitoring of building energy-use, and the least amount of data required for generating acceptable long-term predictions. Four analysis procedures are studied. The short-term monitoring for long-term prediction (SMLP) approach and dry-bulb temperature analysis (DBTA) approach allow determining the best time and duration of the year for in-situ monitoring to be performed based only on the ambient temperature data of the location. Multivariate change-point (MCP) modeling uses simulated/monitored data to determine best monitoring period of the year. This is also used to validate the SMLP and DBTA approaches. The hybrid inverse modeling method-1 predicts energy-use by combining a short dataset of monitored internal loads with a year of utility-bills, and hybrid inverse method-2 predicts long term building performance using utility

  7. Focused Directed Evolution of Aryl-Alcohol Oxidase in Saccharomyces cerevisiae by Using Chimeric Signal Peptides.

    Science.gov (United States)

    Viña-Gonzalez, Javier; Gonzalez-Perez, David; Ferreira, Patricia; Martinez, Angel T; Alcalde, Miguel

    2015-09-01

    Aryl-alcohol oxidase (AAO) is an extracellular flavoprotein that supplies ligninolytic peroxidases with H2O2 during natural wood decay. With a broad substrate specificity and highly stereoselective reaction mechanism, AAO is an attractive candidate for studies into organic synthesis and synthetic biology, and yet the lack of suitable heterologous expression systems has precluded its engineering by directed evolution. In this study, the native signal sequence of AAO from Pleurotus eryngii was replaced by those of the mating α-factor and the K1 killer toxin, as well as different chimeras of both prepro-leaders in order to drive secretion in Saccharomyces cerevisiae. The secretion of these AAO constructs increased in the following order: preproα-AAO > preαproK-AAO > preKproα-AAO > preproK-AAO. The chimeric preαproK-AAO was subjected to focused-directed evolution with the aid of a dual screening assay based on the Fenton reaction. Random mutagenesis and DNA recombination was concentrated on two protein segments (Met[α1]-Val109 and Phe392-Gln566), and an array of improved variants was identified, among which the FX7 mutant (harboring the H91N mutation) showed a dramatic 96-fold improvement in total activity with secretion levels of 2 mg/liter. Analysis of the N-terminal sequence of the FX7 variant confirmed the correct processing of the preαproK hybrid peptide by the KEX2 protease. FX7 showed higher stability in terms of pH and temperature, whereas the pH activity profiles and the kinetic parameters were maintained. The Asn91 lies in the flavin attachment loop motif, and it is a highly conserved residue in all members of the GMC superfamily, except for P. eryngii and P. pulmonarius AAO. The in vitro involution of the enzyme by restoring the consensus ancestor Asn91 promoted AAO expression and stability. PMID:26162870

  8. Post-test calculation and uncertainty analysis of the experiment Quench-07 with the system code Athlet-CD

    International Nuclear Information System (INIS)

    In the frame of developmental assessment and code validation, a post-test calculation of the test QUENCH-07 was performed with ATHLET-CD. The system code ATHLET-CD is being developed for best-estimate simulation of accidents with core degradation and for evaluation of accident management procedures. It applies the detailed models of the thermal-hydraulic code ATHLET in an efficient coupling with dedicated models for core degradation and fission products behaviour. The first step of the work was the simulation of the test QUENCH-07 applying the modelling options recommended in the code User's Manual (reference calculation). The global results of this calculation showed a good agreement with the measured data. This calculation was complemented by a sensitivity analysis in order to investigate the influence of a combined variation of code input parameters on the simulation of the main phenomena observed experimentally. Results of this sensitivity analysis indicate that the main experimental measurements lay within the uncertainty range of the corresponding calculated values. Among the main contributors to the uncertainty of code results are the heat transfer coefficient due to forced convection to superheated steam-argon mixture, the thermal conductivity of the shroud isolation and the external heater rod resistance. Uncertainties on modelling of B4C oxidation do not affect significantly the total calculated hydrogen release rates. (authors)

  9. Post-test calculation and uncertainty analysis of the experiment QUENCH-07 with the system code ATHLET-CD

    Energy Technology Data Exchange (ETDEWEB)

    Austregesilo, Henrique [Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS) mbH, Forschungsinstitute, 85748 Garching (Germany)]. E-mail: Henrique.Austregesilo@grs.de; Bals, Christine [Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS) mbH, Forschungsinstitute, 85748 Garching (Germany); Trambauer, Klaus [Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS) mbH, Forschungsinstitute, 85748 Garching (Germany)

    2007-09-15

    In the frame of developmental assessment and code validation, a post-test calculation of the test QUENCH-07 was performed with ATHLET-CD. The system code ATHLET-CD is being developed for best-estimate simulation of accidents with core degradation and for evaluation of accident management procedures. It applies the detailed models of the thermal-hydraulic code ATHLET in an efficient coupling with dedicated models for core degradation and fission products behaviour. The first step of the work was the simulation of the test QUENCH-07 applying the modelling options recommended in the code User's Manual (reference calculation). The global results of this calculation showed a good agreement with the measured data. This calculation was complemented by a sensitivity analysis in order to investigate the influence of a combined variation of code input parameters on the simulation of the main phenomena observed experimentally. Results of this sensitivity analysis indicate that the main experimental measurements lay within the uncertainty range of the corresponding calculated values. Among the main contributors to the uncertainty of code results are the heat transfer coefficient due to forced convection to superheated steam-argon mixture, the thermal conductivity of the shroud isolation and the external heater rod resistance. Uncertainties on modelling of B{sub 4}C oxidation do not affect significantly the total calculated hydrogen release rates.

  10. Post-test calculation and uncertainty analysis of the experiment QUENCH-07 with the system code ATHLET-CD

    International Nuclear Information System (INIS)

    In the frame of developmental assessment and code validation, a post-test calculation of the test QUENCH-07 was performed with ATHLET-CD. The system code ATHLET-CD is being developed for best-estimate simulation of accidents with core degradation and for evaluation of accident management procedures. It applies the detailed models of the thermal-hydraulic code ATHLET in an efficient coupling with dedicated models for core degradation and fission products behaviour. The first step of the work was the simulation of the test QUENCH-07 applying the modelling options recommended in the code User's Manual (reference calculation). The global results of this calculation showed a good agreement with the measured data. This calculation was complemented by a sensitivity analysis in order to investigate the influence of a combined variation of code input parameters on the simulation of the main phenomena observed experimentally. Results of this sensitivity analysis indicate that the main experimental measurements lay within the uncertainty range of the corresponding calculated values. Among the main contributors to the uncertainty of code results are the heat transfer coefficient due to forced convection to superheated steam-argon mixture, the thermal conductivity of the shroud isolation and the external heater rod resistance. Uncertainties on modelling of B4C oxidation do not affect significantly the total calculated hydrogen release rates

  11. Post-test sensitivity analysis of OECD/CSNI ISP42 panda experiment by Relap5 code

    International Nuclear Information System (INIS)

    The present document deals with Relap5/Mod3.2 analysis of the International Standard Problem (ISP-42) exercise performed in PANDA facility on April 21-22, 1998. PANDA is installed at PSI (Paul Scherrer Institute). PANDA is a large-scale thermal-hydraulic test facility suitable for the simulation of passive containment for Advanced Light Water Reactors (ALWR). The work focuses phase A of the ISP-42 experiment, including the break in the main steam line, and the Passive Containment Cooling System Start-Up. The objective is to investigate the start-up phenomenology of passive cooling system when steam is injected into cold vessel filled with air and to observe the resulting system behavior. A detailed nodalization was set-up at the University of Pisa, in order to model 3-D flow paths with a 1-D code. The comparison between pre-test predictions and experimental data is discussed. Overall time behavior is reasonably well predicted, showing a rather good and robust overall code behavior in the simulation of the global test scenario. The results of a preliminary post-test analysis are discussed, including the comparison with the experimental data. (authors)

  12. Post-test thermal-hydraulic analysis of two intermediate LOCA tests at the ROSA facility including uncertainty evaluation

    International Nuclear Information System (INIS)

    The OECD/NEA ROSA-2 project aims at addressing thermal-hydraulic safety issues relevant for light water reactors by building up an experimental database at the ROSA Large Scale Test Facility (LSTF). The ROSA facility simulates a PWR Westinghouse design with a four-loop configuration and a nominal power of 3423 MWth. Two intermediate break loss-of-coolant-accident (LOCA) experiments (Test 1 and 2) have been carried out during 2010. The two tests were analyzed by using the US-NRC TRACE best estimate code, employing the same nodalization previously used for the simulation of small-break LOCA experiments of the ROSA-1 program. A post-test calculation was performed for each test along with uncertainty analysis providing uncertainty bands for each relevant time trend. Uncertainties in the code modeling capabilities as well as in the initial and boundary conditions were taken into account, following the guidelines and lessons learnt through participation in the OECD/NEA BEMUSE program. Two different versions of the TRACE code were used in the analysis, providing a qualitatively good prediction of the tests. However, both versions showed deficiencies that need to be addressed. The most relevant parameters of the two experimental tests were falling within the computed uncertainty bands. (author)

  13. Patient-specific analysis of post-operative aortic hemodynamics: a focus on thoracic endovascular repair (TEVAR)

    Science.gov (United States)

    Auricchio, F.; Conti, M.; Lefieux, A.; Morganti, S.; Reali, A.; Sardanelli, F.; Secchi, F.; Trimarchi, S.; Veneziani, A.

    2014-10-01

    The purpose of this study is to quantitatively evaluate the impact of endovascular repair on aortic hemodynamics. The study addresses the assessment of post-operative hemodynamic conditions of a real clinical case through patient-specific analysis, combining accurate medical image analysis and advanced computational fluid-dynamics (CFD). Although the main clinical concern was firstly directed to the endoluminal protrusion of the prosthesis, the CFD simulations have demonstrated that there are two other important areas where the local hemodynamics is impaired and a disturbed blood flow is present: the first one is the ostium of the subclavian artery, which is partially closed by the graft; the second one is the stenosis of the distal thoracic aorta. Besides the clinical relevance of these specific findings, this study highlights how CFD analyses allow to observe important flow effects resulting from the specific features of patient vessel geometries. Consequently, our results demonstrate the potential impact of computational biomechanics not only on the basic knowledge of physiopathology, but also on the clinical practice, thanks to a quantitative extraction of knowledge made possible by merging medical data and mathematical models.

  14. Potentially Treatable Disorder Diagnosed Post Mortem by Exome Analysis in a Boy with Respiratory Distress.

    Science.gov (United States)

    Imperatore, Valentina; Mencarelli, Maria Antonietta; Fallerini, Chiara; Bianciardi, Laura; Ariani, Francesca; Furini, Simone; Renieri, Alessandra; Mari, Francesca; Frullanti, Elisa

    2016-01-01

    We highlight the importance of exome sequencing in solving a clinical case of a child who died at 14 months after a series of respiratory crises. He was the half-brother of a girl diagnosed at 7 years with the early-onset seizure variant of Rett syndrome due to CDKL5 mutation. We performed a test for CDKL5 in the boy, which came back negative. Driven by the mother's compelling need for a diagnosis, we moved forward performing whole exome sequencing analysis. Surprisingly, two missense mutations in compound heterozygosity were identified in the RAPSN gene encoding a receptor-associated protein with a key role in clustering and anchoring nicotinic acetylcholine receptors at synaptic sites. This gene is responsible for a congenital form of myasthenic syndrome, a disease potentially treatable with cholinesterase inhibitors. Therefore, an earlier diagnosis in this boy would have led to a better clinical management and prognosis. Our study supports the key role of exome sequencing in achieving a definite diagnosis in severe perinatal diseases, an essential step especially when a specific therapy is available. PMID:26927095

  15. Potentially Treatable Disorder Diagnosed Post Mortem by Exome Analysis in a Boy with Respiratory Distress

    Directory of Open Access Journals (Sweden)

    Valentina Imperatore

    2016-02-01

    Full Text Available We highlight the importance of exome sequencing in solving a clinical case of a child who died at 14 months after a series of respiratory crises. He was the half-brother of a girl diagnosed at 7 years with the early-onset seizure variant of Rett syndrome due to CDKL5 mutation. We performed a test for CDKL5 in the boy, which came back negative. Driven by the mother’s compelling need for a diagnosis, we moved forward performing whole exome sequencing analysis. Surprisingly, two missense mutations in compound heterozygosity were identified in the RAPSN gene encoding a receptor-associated protein with a key role in clustering and anchoring nicotinic acetylcholine receptors at synaptic sites. This gene is responsible for a congenital form of myasthenic syndrome, a disease potentially treatable with cholinesterase inhibitors. Therefore, an earlier diagnosis in this boy would have led to a better clinical management and prognosis. Our study supports the key role of exome sequencing in achieving a definite diagnosis in severe perinatal diseases, an essential step especially when a specific therapy is available.

  16. Post-translational Analysis of Arabidopsis thaliana Proteins in Response to Cyclic Guanosine Monophosphate Treatment

    KAUST Repository

    Parrott, Brian

    2011-12-12

    The introduction of mass spectrometry techniques to the field of biology has made possible the exploration of the proteome as a whole system as opposed to prior techniques, such as anti-body based assays or yeast two-hybrid studies, which were strictly limited to the study of a few proteins at a time. This practice has allowed for a systems biology approach of exploring the proteome, with the possibility of viewing entire pathways over increments of time. In this study, the effect of treating Arabidopsis thaliana suspension culture cells with 3’,5’-cyclic guanosine monophosphate (cGMP), which is a native second messenger, was examined. Samples were collected at four time points and proteins were extracted and enriched for both oxidation and phosphorylation before analysis via mass spectrometry. Preliminary results suggest a tendency towards an increased number of phosphorylated proteins as a result of cGMP treatment. The data also showed a sharp increase in methionine oxidation in response to the treatment, occurring within the first ten minutes. This finding suggests that cGMP may utilize methionine oxidation as a mechanism of signal transduction. As such, this study corroborates a growing body of evidence supporting the inclusion of methionine oxidation in intracellular signaling pathways.

  17. Workplace analysis and radiation protection; L'etudes de poste et radioprotection

    Energy Technology Data Exchange (ETDEWEB)

    Le Guen, B.; Bosquet, Ph.; Chevillard, S.; Gauron, Ch.; Lallemand, J.; Lombard, J.; Menetrier, F.; Feuardent, J.; Maccia, C.; Donadille, L.; Rehel, J.L.; Donnarieix, D.; Garrigou, A.; Gauthereau, V.; Truchi, F.; Chardin, J.; Debouck, F.; Clairand, I.; Amabile, J.Ch.; Vrigneaud, J.M.; Roussille, F.; Witschger, O.; Feuardent, J.; Scanff, P.; Rannou, A.

    2010-07-01

    This document gathers the slides of the available presentations given during this conference day. Fifteen presentations out of 16 are assembled in the document and deal with: 1 - the evolution of doses received by workers (J. Feuardent); 2 - evaluation of extremities dosimetry among interventional radiology practitioners (L. Donadille); 3 - practical guide for the realisation of workplace dosimetry studies presenting a ionizing radiation exposure risk: and example in nuclear medicine (J.L. Rehel); 4 - workplace studies in radiotherapy-curietherapy (D. Donnarieix); 5 - from dosimetry to physical intensity: the case of heat insulation activities (A. Garrigou and C. Piccadaci); 6 - the consideration of human factor during facility modifications (V. Gauthereau); 7 - how to carry out a workplace analysis in gamma-graphy? (F. Truchi); 8 - workplace studies in the framework of dismantling activities (J. Chardin); 9 - team synergy (F. Debouck); 10 - adaptation of individual dosimetry to the workplace: the case of external exposure (I. Clairand); 11 - technical aspects of the evaluation of ionizing radiations exposure induced by a new interventional radiology procedure (J.C. Amabile); 12 - the point of view of a radioprotection skilled person in a nuclear medicine service (J.M. Vrigneaud); 13 - workplace studies for the unique document (F. Roussille); 14 - occupational exposure to manufactured nano-particles: issues and knowledge status (O. Witschger); 15 - toxicological risk of nano-particles: 'health impact'? (S. Chevillard). (J.S.)

  18. Private law enforcement in post-communist Southeastern Europe: An economic analysis of arbitration and execution

    Directory of Open Access Journals (Sweden)

    Schoenfelder Bruno

    2004-01-01

    Full Text Available The very idea of private law enforcement is alien in Southeastern Europe and appears there to be perceived as somewhat extravagant. A thoroughgoing search for the reasons of an extremely limited scope of the private law enforcement is undertaken in the paper. Private law enforcement is seen as taking three basic forms: self-enforcement, enforcement through other agents and illegal means and through specialized agents and with reliance on legal means. Particularly extensive is the analysis of arbitrage, both domestic and international, and of the causes of its modest deployment. Several possible causes are examined to some detail: the lack of knowledge both generally and in the sense of the lacking awareness of the possibility to find recourse to arbitrage, the shortage of lowers and the inadequacy of legal advice, distrust in courts, the preference for secrecy combined with partly operating in the realm of the gray economy, the missing of appellate proceedings and the shortfall in execution. Various factors influence the market share of arbitrage in the mass of litigations through various mechanisms and to a significantly differentiated degree. The paper centers on the various, sufficiently numerous components of these mechanisms. The stress is placed upon the still subdued power of reputation capital due mostly to a highly unstable macroeconomic situation, weakly and recently institutionalized property relations and, as a consequence, short decision making horizon. A new law enforcement mechanism, based on notaries as specific private enforcers and emerging in Croatia, is surveyed with an account of its advantages and disadvantages.

  19. In-pile thermocycling testing and post-test analysis of beryllium divertor mockups

    Energy Technology Data Exchange (ETDEWEB)

    Giniatulin, R.; Mazul, I. [Efremov Inst., St. Petersburg (Russian Federation); Melder, R.; Pokrovsky, A.; Sandakov, V.; Shiuchkin, A.

    1998-01-01

    The main damaging factors which impact the ITER divertor components are neutron irradiation, cyclic surface heat loads and hydrogen environment. One of the important questions in divertor mockups development is the reliability of beryllium/copper joints and the beryllium resistance under neutron irradiation and thermal cycling. This work presents the experiment, where neutron irradiation and thermocyclic heat loads were applied simultaneously for two beryllium/copper divertor mockups in a nuclear reactor channel to simulate divertor operational conditions. Two mockups with different beryllium grades were mounted facing each other with the tantalum heater placed between them. This device was installed in the active zone of the nuclear reactor SM-2 (Dimitrovgrad, Russia) and the tantalum block was heated by neutron irradiation up to a high temperature. The main part of the heat flux from the tantalum surface was transported to the beryllium surface through hydrogen, as a result the heat flux loaded two mockups simultaneously. The mockups were cooled by reactor water. The device was lowered to the active zone so as to obtain the heating regime and to provide cooling lifted. This experiment was performed under the following conditions: tantalum heater temperature - 1950degC; hydrogen environment -1000 Pa; surface heat flux density -3.2 MW/m{sup 2}; number of thermal cycles (lowering and lifting) -101; load time in each cycle - 200-5000 s; dwell time (no heat flux, no neutrons) - 300-2000 s; cooling water parameters: v - 1 m/s, Tin - 50degC, Pin - 5 MPa; neutron fluence -2.5 x 10{sup 20} cm{sup -2} ({approx}8 years of ITER divertor operation from the start up). The metallographic analysis was performed after experiment to investigate the beryllium and beryllium/copper joint structures, the results are presented in the paper. (author)

  20. Chimeric Peptides as Implant Functionalization Agents for Titanium Alloy Implants with Antimicrobial Properties

    Science.gov (United States)

    Yucesoy, Deniz T.; Hnilova, Marketa; Boone, Kyle; Arnold, Paul M.; Snead, Malcolm L.; Tamerler, Candan

    2015-04-01

    Implant-associated infections can have severe effects on the longevity of implant devices and they also represent a major cause of implant failures. Treating these infections associated with implants by antibiotics is not always an effective strategy due to poor penetration rates of antibiotics into biofilms. Additionally, emerging antibiotic resistance poses serious concerns. There is an urge to develop effective antibacterial surfaces that prevent bacterial adhesion and proliferation. A novel class of bacterial therapeutic agents, known as antimicrobial peptides (AMPs), are receiving increasing attention as an unconventional option to treat septic infection, partly due to their capacity to stimulate innate immune responses and for the difficulty of microorganisms to develop resistance towards them. While host and bacterial cells compete in determining the ultimate fate of the implant, functionalization of implant surfaces with AMPs can shift the balance and prevent implant infections. In the present study, we developed a novel chimeric peptide to functionalize the implant material surface. The chimeric peptide simultaneously presents two functionalities, with one domain binding to a titanium alloy implant surface through a titanium-binding domain while the other domain displays an antimicrobial property. This approach gains strength through control over the bio-material interfaces, a property built upon molecular recognition and self-assembly through a titanium alloy binding domain in the chimeric peptide. The efficiency of chimeric peptide both in-solution and absorbed onto titanium alloy surface was evaluated in vitro against three common human host infectious bacteria, Streptococcus mutans, Staphylococcus epidermidis, and Escherichia coli. In biological interactions such as occur on implants, it is the surface and the interface that dictate the ultimate outcome. Controlling the implant surface by creating an interface composed chimeric peptides may therefore

  1. Murine immune responses to a Plasmodium vivax-derived chimeric recombinant protein expressed in Brassica napus

    Directory of Open Access Journals (Sweden)

    Chung Nam-Jun

    2011-04-01

    Full Text Available Abstract Background To develop a plant-based vaccine against Plasmodium vivax, two P. vivax candidate proteins were chosen. First, the merozoite surface protein-1 (MSP-1, a major asexual blood stage antigen that is currently considered a strong vaccine candidate. Second, the circumsporozoite protein (CSP, a component of sporozoites that contains a B-cell epitope. Methods A synthetic chimeric recombinant 516 bp gene encoding containing PvMSP-1, a Pro-Gly linker motif, and PvCSP was synthesized; the gene, named MLC, encoded a total of 172 amino acids. The recombinant gene was modified with regard to codon usage to optimize gene expression in Brassica napus. The Ti plasmid inducible gene transfer system was used for MLC chimeric recombinant gene expression in B. napus. Gene expression was confirmed by polymerase chain reaction (PCR, beta-glucuronidase reporter gene (GUS assay, and Western blot. Results The MLC chimeric recombinant protein expressed in B. napus had a molecular weight of approximately 25 kDa. It exhibited a clinical sensitivity of 84.21% (n = 38 and a clinical specificity of 100% (n = 24 as assessed by enzyme-linked immunosorbent assay (ELISA. Oral immunization of BALB/c mice with MLC chimeric recombinant protein successfully induced antigen-specific IgG1 production. Additionally, the Th1-related cytokines IL-12 (p40, TNF, and IFN-γ were significantly increased in the spleens of the BALB/c mice. Conclusions The chimeric MLC recombinant protein produced in B. napus has potential as both as an antigen for diagnosis and as a valuable vaccine candidate for oral immunization against vivax malaria.

  2. The expression and genetic immunization of chimeric fragment of Hantaan virus M and S segments

    International Nuclear Information System (INIS)

    Hemorrhagic fever with renal syndrome (HFRS), which is characterized by severe symptoms and high mortality, is caused by hantavirus. There are still no effective prophylactic vaccines directed to HFRS until now. In this research, we fused expressed G2 fragment of M segment and 0.7 kb fragment of S segment. We expect it could be a candidate vaccine. Chimeric gene G2S0.7 was first expressed in prokaryotic expression system pGEX-4T. After inducing expressed fusion proteins, GST-G2S0.7 was induced and its molecular weight was about 100 kDa. Meanwhile, the fusion protein kept the activity of its parental proteins. Further, BALB/c mice were vaccinated by the chimeric gene. ELISA, cell microculture neutralization test in vitro were used to detect the humoral immune response in immunized BALB/c mice. Lymphocyte proliferation assay was used to detect the cellular immune response. The results showed that the chimeric gene could simultaneously evoke specific antibody against nucleocapsid protein (NP) and glycoprotein (GP). And the immunized mice of every group elicited neutralizing antibodies with different titers. But the titers were low. Lymphocyte proliferation assay results showed that the stimulation indexes of splenocytes of chimeric gene to NP and GP were significantly higher than that of control. It suggested that the chimeric gene of Hantaan virus containing G2 fragment of M segment and 0.7 kb fragment of S segment could directly elicit specific anti-Hantaan virus humoral and cellular immune response in BALB/c mice

  3. Chimeric SV40 virus-like particles induce specific cytotoxicity and protective immunity against influenza A virus without the need of adjuvants

    International Nuclear Information System (INIS)

    Virus-like particles (VLPs) are a promising vaccine platform due to the safety and efficiency. However, it is still unclear whether polyomavirus-based VLPs are useful for this purpose. Here, we attempted to evaluate the potential of polyomavirus VLPs for the antiviral vaccine using simian virus 40 (SV40). We constructed chimeric SV40-VLPs carrying an HLA-A⁎02:01-restricted, cytotoxic T lymphocyte (CTL) epitope derived from influenza A virus. HLA-A⁎02:01-transgenic mice were then immunized with the chimeric SV40-VLPs. The chimeric SV40-VLPs effectively induced influenza-specific CTLs and heterosubtypic protection against influenza A viruses without the need of adjuvants. Because DNase I treatment of the chimeric SV40-VLPs did not disrupt CTL induction, the intrinsic adjuvant property may not result from DNA contaminants in the VLP preparation. In addition, immunization with the chimeric SV40-VLPs generated long-lasting memory CTLs. We here propose that the chimeric SV40-VLPs harboring an epitope may be a promising CTL-based vaccine platform with self-adjuvant properties. - Highlights: • We constructed chimeric SV40-VLPs carrying an influenza virus-derived CTL epitope. • Chimeric SV40-VLPs induce influenza-specific CTLs in mice without adjuvants. • Chimeric SV40-VLPs induce heterosubtypic protection against influenza A viruses. • Chimeric SV40-VLPs induce long-lasting memory CTLs. • Chimeric SV40-VLPs is a promising vaccine platform with self-adjuvant properties

  4. Evaluation of Intracellular Signaling Downstream Chimeric Antigen Receptors.

    Directory of Open Access Journals (Sweden)

    Hannah Karlsson

    Full Text Available CD19-targeting CAR T cells have shown potency in clinical trials targeting B cell leukemia. Although mainly second generation (2G CARs carrying CD28 or 4-1BB have been investigated in patients, preclinical studies suggest that third generation (3G CARs with both CD28 and 4-1BB have enhanced capacity. However, little is known about the intracellular signaling pathways downstream of CARs. In the present work, we have analyzed the signaling capacity post antigen stimulation in both 2G and 3G CARs. 3G CAR T cells expanded better than 2G CAR T cells upon repeated stimulation with IL-2 and autologous B cells. An antigen-driven accumulation of CAR+ cells was evident post antigen stimulation. The cytotoxicity of both 2G and 3G CAR T cells was maintained by repeated stimulation. The phosphorylation status of intracellular signaling proteins post antigen stimulation showed that 3G CAR T cells had a higher activation status than 2G. Several proteins involved in signaling downstream the TCR were activated, as were proteins involved in the cell cycle, cell adhesion and exocytosis. In conclusion, 3G CAR T cells had a higher degree of intracellular signaling activity than 2G CARs which may explain the increased proliferative capacity seen in 3G CAR T cells. The study also indicates that there may be other signaling pathways to consider when designing or evaluating new generations of CARs.

  5. Evaluation of Intracellular Signaling Downstream Chimeric Antigen Receptors.

    Science.gov (United States)

    Karlsson, Hannah; Svensson, Emma; Gigg, Camilla; Jarvius, Malin; Olsson-Strömberg, Ulla; Savoldo, Barbara; Dotti, Gianpietro; Loskog, Angelica

    2015-01-01

    CD19-targeting CAR T cells have shown potency in clinical trials targeting B cell leukemia. Although mainly second generation (2G) CARs carrying CD28 or 4-1BB have been investigated in patients, preclinical studies suggest that third generation (3G) CARs with both CD28 and 4-1BB have enhanced capacity. However, little is known about the intracellular signaling pathways downstream of CARs. In the present work, we have analyzed the signaling capacity post antigen stimulation in both 2G and 3G CARs. 3G CAR T cells expanded better than 2G CAR T cells upon repeated stimulation with IL-2 and autologous B cells. An antigen-driven accumulation of CAR+ cells was evident post antigen stimulation. The cytotoxicity of both 2G and 3G CAR T cells was maintained by repeated stimulation. The phosphorylation status of intracellular signaling proteins post antigen stimulation showed that 3G CAR T cells had a higher activation status than 2G. Several proteins involved in signaling downstream the TCR were activated, as were proteins involved in the cell cycle, cell adhesion and exocytosis. In conclusion, 3G CAR T cells had a higher degree of intracellular signaling activity than 2G CARs which may explain the increased proliferative capacity seen in 3G CAR T cells. The study also indicates that there may be other signaling pathways to consider when designing or evaluating new generations of CARs. PMID:26700307

  6. Human risk contributions in process industry: Guides for their pre-identification in well-structured activities and for post-incident analysis

    International Nuclear Information System (INIS)

    The report should be considered a guide for the treating of human errors: for identifying their possibilities of occurrence when designing well-structured human tasks and for their improvement when they occur in reality. For these purposes a strong coupling between predictive and retrospective analysis is emphasized: In order to control human errors, post-incident analysis of cases with human errors in a given industrial plant should be performed as means of feedback from reality for the verification of results of predictive analysis and also as general means of identifying and improving such human errors which cannot be expected covered by predictive analysis. Primarily, the guide addresses people with a knowledge of the technical plant in question and involved in the safety-oriented design and improvement of human activities and without a particular human factors background. The main report describes the procedures for post-incident analysis and for Work Analysis, which is a search strategy developed for well-defined activities, e.g. test and calibration, and constitutes a formalized procedure for the pre-identification of relevant human errors leading to a lack of task result and/or to immediate effects not covered by the lack of task result itself. Work Analysis and the post-incident analysis procedure are based on a common description system for human malfunctions. This system is explained in appendix and so are its underlying models and way of reasoning. Finally, a word index is provided for supporting the reader. (author)

  7. Three-dimensional finite element analysis of weakened roots restored with different cements in combination with titanium alloy posts

    Institute of Scientific and Technical Information of China (English)

    LI Li-li; WANG Zhong-yi; BAI Zhong-cheng; MAO Yong; GAO Bo; XIN Hai-tao; ZHOU Bing; ZHANG Yong; LIU Bing

    2006-01-01

    Background It is very difficult and relatively unpredictable to preserve and restore severely weakened pulpless roots. To provide much needed benefit basis for clinical practice, this study was carried out to analyze the stress distribution in weakened roots restored with different cements in combination with titanium alloy posts. Finite element analysis (FEA) was employed in the study.Methods A pseudo three-dimensional model of a maxillary central incisor with flared root canal, theoretically restored with titanium alloy posts in combination with different cements, was established. The analysis was performed by use of ANSYS software. The tooth was assumed to be isotropic, homogenous and elastic. A load of 100 N at an angle of 45°to the longitudinal axis was applied at the palatal surface of the crown. The distributions of stresses in weakened roots filled with cements of different elastic modulus were analyzed by the three-dimensional FEA model.Results Several stress trends were observed when the stress cloud atlas obtained in the study was analyzed. With the increase of the elastic modulus of cements from 1.8 GPa to 22.4 GPa, the stress values in dentin decreased from 39.58 MPa to 31.43 MPa and from 24.51 MPa to 20.76 MPa (respectively, for maximum principle stress values and Von Mises stress values). When Panavia F and zinc phosphate cement were used, the stress peak values in dentin were very small with no significant difference observed, and the Von Mises stress values were 20.87 MPa and 20.76 MPa respectively. On the other hand, maximum principle stress value and Von Mises stress value in cement layer increased with the increase of the elastic modulus of cements.Conclusions The result of this study demonstrated that elastic modulus was indeed one of the important parameters to evaluate property of the cements. Our three-dimensional FEA model study also found that the cement with elastic modulus similar to that of dentin could reinforce weakened root and

  8. Phenology Analysis of Forest Vegetation to Environmental Variables during - and Post-Monsoon Seasons in Western Himalayan Region of India

    Science.gov (United States)

    Khare, S.; Latifi, H.; Ghosh, K.

    2016-06-01

    To assess the phenological changes in Moist Deciduous Forest (MDF) of western Himalayan region of India, we carried out NDVI time series analysis from 2013 to 2015 using Landsat 8 OLI data. We used the vegetation index differencing method to calculate the change in NDVI (NDVIchange) during pre and post monsoon seasons and these changes were used to assess the phenological behaviour of MDF by taking the effect of a set of environmental variables into account. To understand the effect of environmental variables on change in phenology, we designed a linear regression analysis with sample-based NDVIchange values as the response variable and elevation aspect, and Land Surface Temperature (LST) as explanatory variables. The Landsat-8 derived phenology transition stages were validated by calculating the phenology variation from Nov 2008 to April 2009 using Landsat-7 which has the same spatial resolution as Landsat-8. The Landsat-7 derived NDVI trajectories were plotted in accordance with MODIS derived phenology stages (from Nov 2008 to April 2009) of MDF. Results indicate that the Landsat -8 derived NDVI trajectories describing the phenology variation of MDF during spring, monsoon autumn and winter seasons agreed closely with Landsat-7 and MODIS derived phenology transition from Nov 2008 to April 2009. Furthermore, statistical analysis showed statistically significant correlations (p < 0.05) amongst the environmental variables and the NDVIchange between full greenness and maximum frequency stage of Onset of Greenness (OG) activity.. The major change in NDVI was observed in medium (600 to 650 m) and maximum (650 to 750 m) elevation areas. The change in LST showed also to be highly influential. The results of this study can be used for large scale monitoring of difficult-to-reach mountainous forests, with additional implications in biodiversity assessment. By means of a sufficient amount of available cloud-free imagery, detailed phenological trends across mountainous

  9. Improving thermal model prediction through statistical analysis of irradiation and post-irradiation data from AGR experiments

    International Nuclear Information System (INIS)

    As part of the High Temperature Reactors (HTR) R and D program, a series of irradiation tests, designated as Advanced Gas-cooled Reactor (AGR), have been defined to support development and qualification of fuel design, fabrication process, and fuel performance under normal operation and accident conditions. The AGR tests employ fuel compacts placed in a graphite cylinder shrouded by a steel capsule and instrumented with thermocouples (TC) embedded in graphite blocks enabling temperature control. While not possible to obtain by direct measurements in the tests, crucial fuel conditions (e.g., temperature, neutron fast fluence, and burnup) are calculated using core physics and thermal modeling codes. This paper is focused on AGR test fuel temperature predicted by the ABAQUS code's finite element-based thermal models. The work follows up on a previous study, in which several statistical analysis methods were adapted, implemented in the NGNP Data Management and Analysis System (NDMAS), and applied for qualification of AGR-1 thermocouple data. Abnormal trends in measured data revealed by the statistical analysis are traced to either measuring instrument deterioration or physical mechanisms in capsules that may have shifted the system thermal response. The main thrust of this work is to exploit the variety of data obtained in irradiation and post-irradiation examination (PIE) for assessment of modeling assumptions. As an example, the uneven reduction of the control gas gap in Capsule 5 found in the capsule metrology measurements in PIE helps identify mechanisms other than TC drift causing the decrease in TC readings. This suggests a more physics-based modification of the thermal model that leads to a better fit with experimental data, thus reducing model uncertainty and increasing confidence in the calculated fuel temperatures of the AGR-1 test

  10. Lenient vs. strict rate control in patients with atrial fibrillation and heart failure : a post-hoc analysis of the RACE II study

    NARCIS (Netherlands)

    Mulder, Bart A.; Van Veldhuisen, Dirk J.; Crijns, Harry J. G. M.; Tijssen, Jan G. P.; Hillege, Hans L.; Alings, Marco; Rienstra, Michel; Groenveld, Hessel F.; Van den Berg, Maarten P.; Van Gelder, Isabelle C.

    2013-01-01

    AIMS: It is unknown whether lenient rate control is an acceptable strategy in patients with AF and heart failure. We evaluated differences in outcome in patients with AF and heart failure treated with lenient or strict rate control. METHODS AND RESULTS: This post-hoc analysis of the RACE II trial in

  11. Analysis of Suicidal Behaviour in Israeli Veterans and Terror Victims with Post-Traumatic Stress Disorder by Using the Computerised Gottschalk-Gleser Scales

    Science.gov (United States)

    Galor, Sharon; Hentschel, Uwe

    2009-01-01

    The primary objective of this study was to identify the vulnerability factors for suicide attempts in an Israeli sample, with the help of the Gottschalk-Gleser content analysis scales. The respondents were divided into four groups: suicide attempters; controls; post-traumatic stress disorder and depressed patients who did not report suicidal…

  12. Validation of CBZ code system for post-irradiation examination analysis and sensitivity analysis of (n,γ) branching ratio

    International Nuclear Information System (INIS)

    A code system CBZ is being developed in Hokkaido University. In order to validate it, PIE data, which are nuclide composition data of a spent fuel, have been analyzed with CBZ. The validity is evaluated as ratios of the calculation values to the experimental ones (C/E ratios). Differences between experimental values and calculation ones are smaller than 20% except some nuclides. Thus this code system is validated. Additionally, we evaluate influence of change of (n,γ) branching ratio on inventories of fission products and actinides. As a result, branching ratios of Sb-121, Pm-147, and Am-241 influence inventories of several nuclides. We perform PIE analysis using different (n,γ) branching ratio data from the ORIGEN-2 library, JNDC-Ver.2, and JEFF-3.1A, and find that differences in (n,γ) branching ratios between different nuclear libraries have a non-negligible influence on inventories of several nuclides. (author)

  13. Serotype chimeric oncolytic adenovirus coding for GM-CSF for treatment of sarcoma in rodents and humans.

    Science.gov (United States)

    Bramante, Simona; Koski, Anniina; Kipar, Anja; Diaconu, Iulia; Liikanen, Ilkka; Hemminki, Otto; Vassilev, Lotta; Parviainen, Suvi; Cerullo, Vincenzo; Pesonen, Saila K; Oksanen, Minna; Heiskanen, Raita; Rouvinen-Lagerström, Noora; Merisalo-Soikkeli, Maiju; Hakonen, Tiina; Joensuu, Timo; Kanerva, Anna; Pesonen, Sari; Hemminki, Akseli

    2014-08-01

    Sarcomas are a relatively rare cancer, but often incurable at the late metastatic stage. Oncolytic immunotherapy has gained attention over the past years, and a wide range of oncolytic viruses have been delivered via intratumoral injection with positive safety and promising efficacy data. Here, we report preclinical and clinical results from treatment of sarcoma with oncolytic adenovirus Ad5/3-D24-GMCSF (CGTG-102). Ad5/3-D24-GMCSF is a serotype chimeric oncolytic adenovirus coding for human granulocyte-macrophage colony-stimulating factor (GM-CSF). The efficacy of Ad5/3-D24-GMCSF was evaluated on a panel of soft-tissue sarcoma (STS) cell lines and in two animal models. Sarcoma specific human data were also collected from the Advanced Therapy Access Program (ATAP), in preparation for further clinical development. Efficacy was seen in both in vitro and in vivo STS models. Fifteen patients with treatment-refractory STS (13/15) or primary bone sarcoma (2/15) were treated in ATAP, and treatments appeared safe and well-tolerated. A total of 12 radiological RECIST response evaluations were performed, and two cases of minor response, six cases of stable disease and four cases of progressive disease were detected in patients progressing prior to virus treatment. Overall, the median survival time post treatment was 170 days. One patient is still alive at 1,459 days post virus treatment. In summary, Ad5/3-D24-GMCSF appears promising for the treatment of advanced STS; a clinical trial for treatment of refractory injectable solid tumors including STS is ongoing. PMID:24374597

  14. (Re)Conceptualizing Higher Education in Post-Disaster Contexts: A Processual Analysis of Diaspora Engagement in Haiti's Reconstruction

    Science.gov (United States)

    Cela Hamm, Toni

    2016-01-01

    In post-disaster and post-conflict societies, critical threshold events trigger or intensify diaspora mobilization and engagement in their homelands. Taking the 2010 earthquake as a "critical event" that has transformed the course of Haitian society, this study builds on existing research on diaspora influence on homeland development by…

  15. Global analysis of myocardial peptides containing cysteines with irreversible sulfinic and sulfonic Acid post-translational modifications

    DEFF Research Database (Denmark)

    Paulech, Jana; Liddy, Kiersten A; Engholm-Keller, Kasper;

    2015-01-01

    Cysteine (Cys) oxidation is a crucial post-translational modification (PTM) associated with redox signaling and oxidative stress. As Cys is highly reactive to oxidants it forms a range of post-translational modifications, some that are biologically reversible (e.g. disulfides, Cys sulfenic acid...

  16. Analysis of Swine (Sus scrofa DNA from Blowfly (Chrysomya megacephala Larval Guts after Post-Feeding Periods by PCR Technique

    Directory of Open Access Journals (Sweden)

    Monthira MONTHATONG

    2016-02-01

    Full Text Available This research aimed to investigate the DNA of food sources taken from blowfly (Chrysomya megacephala larval guts, for application in forensic entomology. The muscle samples of domestic swine (Sus scrofa, decomposed in 2 conditions, in air and drowning, were used as case studies. Adult blowflies were allowed to lay eggs on the meat samples. Then, the third instar larvae were identified as C. megacephala, then transferred to non-meat food and collected every 6 h up to 36 h. Total DNA extraction was performed by the Phenol-Chloroform method, and used as DNA templates in a Polymerase Chain Reaction (PCR test. The PCR amplified swine partial cytochrome b (cyt b genes with 203 bp expected size. The results showed that the swine DNA from blowfly larvae grown on decayed tissue under air and drowning conditions could be detected by PCR after larvae post-feeding up to 18 and 36 h, respectively. The sequence analysis revealed that the forward and reverse strands undergo 94 and 98 % identities of the swine cyt b gene sequence retrieved from nucleotide databases. This study suggests that mitochondrial DNA of the food source from insect guts can be detected at limited times after feeding, combined with the condition of the decayed tissues.

  17. The contribution of turmeric research and development In the economy of Bangladesh: an ex-post analysis

    Directory of Open Access Journals (Sweden)

    MK Hasan

    2014-06-01

    Full Text Available The study estimated the benefit and rates of returns to investment on turmeric research and development in Bangladesh. The Economic Surplus Model with ex-post analysis was used to determine the returns to investment and their distribution between the production and consumption. Several discounting techniques were also used to assess the efficiency of turmeric research. The adoption rate was found increasing trend over the period. The yield of BARI developed modern varieties of turmeric was 41 to 73% higher than those of the local variety. Society got net benefit Tk. 9333.88 million from the investment of turmeric research and extension. The net present value (NPV and present value of research cost (PVRC were estimated at Tk. 1200.84 and 157.88, respectively. The internal rate of return (IRR and benefit cost ratio (BCR were estimated to be 68% and 10.45, respectively indicated investment on turmeric research and development was a good and profitable investment. Seed production programme of turmeric should be taken largely to increase production by increasing area adoption.

  18. Online Social Support for Patients with Multiple Sclerosis: A Thematic Analysis of Messages Posted to a Virtual Support Community

    Directory of Open Access Journals (Sweden)

    Masoumeh Abbasi Shavazi

    2016-07-01

    Full Text Available Background: Currently with the emergence of the Internet, patients have an opportunity to exchange social support online. However, little attention has been devoted to different dimensions of online social support exchanged in virtual support communities for patients with multiple sclerosis (MS. Methods: To provide a rich insight, the aim of this qualitative study was to explore and categorize different dimensions of online social support in messages exchanged in a virtual support community for patients with MS. A total of 548 posted messages created during one year period were selected using purposive sampling to consider the maximum variation sampling. Prior-research-driven thematic analysis was then conducted. In this regard, we used the Cutruna and Suhr’s coding system. The messages that could not be categorized with the used coding system were thematically analyzed to explore new additional social support themes. Results: The results showed that various forms of social support including informational, emotional, network, esteem and tangible support were exchanged. Moreover, new additional social support themes including sharing personal experiences, sharing coping strategies and spiritual support emerged in this virtual support community. Conclusion: The wide range of online social support exchanged in the virtual support community can be regarded as a supplementary source of social support for patients with MS. Future researches can examine online social support more comprehensively considering additional social support themes emerging in the present study.

  19. Two-stage anaerobic and post-aerobic mesophilic digestion of sewage sludge: Analysis of process performance and hygienization potential.

    Science.gov (United States)

    Tomei, M Concetta; Mosca Angelucci, Domenica; Levantesi, Caterina

    2016-03-01

    Sequential anaerobic-aerobic digestion has been demonstrated to be effective for enhanced sludge stabilization, in terms of increased solid reduction and improvement of sludge dewaterability. In this study, we propose a modified version of the sequential anaerobic-aerobic digestion process by operating the aerobic step under mesophilic conditions (T=37 °C), in order to improve the aerobic degradation kinetics of soluble and particulate chemical oxygen demand (COD). Process performance has been assessed in terms of "classical parameters" such as volatile solids (VS) removal, biogas production, COD removal, nitrogen species, and polysaccharide and protein fate. The aerobic step was operated under intermittent aeration to achieve nitrogen removal. Aerobic mesophilic conditions consistently increased VS removal, providing 32% additional removal vs. 20% at 20 °C. Similar results were obtained for nitrogen removal, increasing from 64% up to 99% at the higher temperature. Improved sludge dewaterability was also observed with a capillary suction time decrease of ~50% during the mesophilic aerobic step. This finding may be attributable to the decreased protein content in the aerobic digested sludge. The post-aerobic digestion exerted a positive effect on the reduction of microbial indicators while no consistent improvement of hygienization related to the increased temperature was observed. The techno-economic analysis of the proposed digestion layout showed a net cost saving for sludge disposal estimated in the range of 28-35% in comparison to the single-phase anaerobic digestion. PMID:26760266

  20. Stability of chromosome aberrations with post-irradiation time. Implications in retrospective biodosimetry. Chromosome aberration analysis in retrospective biodosimetry

    International Nuclear Information System (INIS)

    The aim of the present study was to evaluate the persistence chromosome aberrations induced by three doses of X-rays. For this purpose fluorescence in situ hybridisation (FISH) painting and multiplex FISH (mFISH) techniques have been applied to a long-term culture of irradiated cells. By painting, at 2 Gy the frequency of apparently simple translocations remained almost invariable during all the culture, whereas at 4 Gy a rapid decline was observed between the first and the second sample, followed by a slight decrease until the end of the culture. Apparently simple dicentrics and complex aberrations disappeared after the first sample at 2 and 4 Gy. When simple aberrations analysed by mFISH are considered, at 2 Gy the frequency of complete plus one-way translocations remained invariable between the first and last sample, but at 4 Gy a 60% decline was observed. True incomplete simple translocations disappeared at 2 and 4 Gy. The analysis by mFISH showed that the frequency of complex aberrations and their complexity increased with dose and tends to disappear in the last sample. Our results indicate that the dose influence on the decrease of the frequency of simple translocations with post-irradiation time cannot be fully explained by the disappearance of true incomplete translocations and complex aberrations. (author)